LOCUS       DAZKHC020000001       133111 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZKHC020000001 DAZKHC020000000
VERSION     DAZKHC020000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 133111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 133111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 133111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..133111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.001"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..262
                     /locus_tag="ACHOSM_RS00005"
                     /old_locus_tag="pgaptmp_000001"
     CDS             <1..262
                     /locus_tag="ACHOSM_RS00005"
                     /old_locus_tag="pgaptmp_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HGU4556616.1"
                     /translation="NAALMSERPIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEEL
                     WSPVRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(298..1254)
                     /gene="ybhN"
                     /locus_tag="ACHOSM_RS00010"
                     /old_locus_tag="pgaptmp_000002"
     CDS             complement(298..1254)
                     /gene="ybhN"
                     /locus_tag="ACHOSM_RS00010"
                     /old_locus_tag="pgaptmp_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HGU4556617.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSENYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1254..2495)
                     /gene="clsB"
                     /locus_tag="ACHOSM_RS00015"
                     /old_locus_tag="pgaptmp_000003"
     CDS             complement(1254..2495)
                     /gene="clsB"
                     /locus_tag="ACHOSM_RS00015"
                     /old_locus_tag="pgaptmp_000003"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HGU4556618.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPRQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2492..3253)
                     /gene="ybhP"
                     /locus_tag="ACHOSM_RS00020"
                     /old_locus_tag="pgaptmp_000004"
     CDS             complement(2492..3253)
                     /gene="ybhP"
                     /locus_tag="ACHOSM_RS00020"
                     /old_locus_tag="pgaptmp_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU4556619.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFVYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3386..3796
                     /gene="ybhQ"
                     /locus_tag="ACHOSM_RS00025"
                     /old_locus_tag="pgaptmp_000005"
     CDS             3386..3796
                     /gene="ybhQ"
                     /locus_tag="ACHOSM_RS00025"
                     /old_locus_tag="pgaptmp_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HGU4556620.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3758..4864)
                     /gene="ybhR"
                     /locus_tag="ACHOSM_RS00030"
                     /old_locus_tag="pgaptmp_000006"
     CDS             complement(3758..4864)
                     /gene="ybhR"
                     /locus_tag="ACHOSM_RS00030"
                     /old_locus_tag="pgaptmp_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4556621.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     CIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4875..6008)
                     /gene="ybhS"
                     /locus_tag="ACHOSM_RS00035"
                     /old_locus_tag="pgaptmp_000007"
     CDS             complement(4875..6008)
                     /gene="ybhS"
                     /locus_tag="ACHOSM_RS00035"
                     /old_locus_tag="pgaptmp_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4556622.1"
                     /translation="MSNPILSWRRVRALCVKETRQLVRDPSSWLIAVVIPLLLLFVFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6001..7737)
                     /gene="ybhF"
                     /locus_tag="ACHOSM_RS00040"
                     /old_locus_tag="pgaptmp_000008"
     CDS             complement(6001..7737)
                     /gene="ybhF"
                     /locus_tag="ACHOSM_RS00040"
                     /old_locus_tag="pgaptmp_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU4556623.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7730..8725)
                     /gene="hlyD"
                     /locus_tag="ACHOSM_RS00045"
                     /old_locus_tag="pgaptmp_000009"
     CDS             complement(7730..8725)
                     /gene="hlyD"
                     /locus_tag="ACHOSM_RS00045"
                     /old_locus_tag="pgaptmp_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HGU4556624.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(8728..9399)
                     /gene="cecR"
                     /locus_tag="ACHOSM_RS00050"
                     /old_locus_tag="pgaptmp_000010"
     CDS             complement(8728..9399)
                     /gene="cecR"
                     /locus_tag="ACHOSM_RS00050"
                     /old_locus_tag="pgaptmp_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HGU4556625.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9628..10992
                     /gene="rhlE"
                     /locus_tag="ACHOSM_RS00055"
                     /old_locus_tag="pgaptmp_000011"
     CDS             9628..10992
                     /gene="rhlE"
                     /locus_tag="ACHOSM_RS00055"
                     /old_locus_tag="pgaptmp_000011"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HGU4556626.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11224..11706)
                     /gene="ybiA"
                     /locus_tag="ACHOSM_RS00060"
                     /old_locus_tag="pgaptmp_000012"
                     /pseudo
     CDS             complement(11224..11706)
                     /gene="ybiA"
                     /locus_tag="ACHOSM_RS00060"
                     /old_locus_tag="pgaptmp_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
     gene            11826..13976
                     /gene="dinG"
                     /locus_tag="ACHOSM_RS00065"
                     /old_locus_tag="pgaptmp_000013"
     CDS             11826..13976
                     /gene="dinG"
                     /locus_tag="ACHOSM_RS00065"
                     /old_locus_tag="pgaptmp_000013"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HGU4556627.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14004..14966
                     /gene="ybiB"
                     /locus_tag="ACHOSM_RS00070"
                     /old_locus_tag="pgaptmp_000014"
     CDS             14004..14966
                     /gene="ybiB"
                     /locus_tag="ACHOSM_RS00070"
                     /old_locus_tag="pgaptmp_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HGU4556628.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTACSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLERVNQAF"
     gene            15107..16192
                     /gene="hcxB"
                     /locus_tag="ACHOSM_RS00075"
                     /old_locus_tag="pgaptmp_000015"
     CDS             15107..16192
                     /gene="hcxB"
                     /locus_tag="ACHOSM_RS00075"
                     /old_locus_tag="pgaptmp_000015"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HGU4556629.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQGFCQQLAS"
     gene            16258..16317
                     /gene="ybiE"
                     /locus_tag="ACHOSM_RS00080"
                     /old_locus_tag="pgaptmp_000016"
     CDS             16258..16317
                     /gene="ybiE"
                     /locus_tag="ACHOSM_RS00080"
                     /old_locus_tag="pgaptmp_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HGU4556630.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(16420..16680)
                     /gene="ybiJ"
                     /locus_tag="ACHOSM_RS00085"
                     /old_locus_tag="pgaptmp_000017"
     CDS             complement(16420..16680)
                     /gene="ybiJ"
                     /locus_tag="ACHOSM_RS00085"
                     /old_locus_tag="pgaptmp_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HGU4556631.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(16945..17211)
                     /gene="ybiI"
                     /locus_tag="ACHOSM_RS00090"
                     /old_locus_tag="pgaptmp_000018"
     CDS             complement(16945..17211)
                     /gene="ybiI"
                     /locus_tag="ACHOSM_RS00090"
                     /old_locus_tag="pgaptmp_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HGU4556632.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDSQKPAYTGYNRRGSKDSQLR"
     gene            complement(17285..17962)
                     /gene="ybiX"
                     /locus_tag="ACHOSM_RS00095"
                     /old_locus_tag="pgaptmp_000019"
     CDS             complement(17285..17962)
                     /gene="ybiX"
                     /locus_tag="ACHOSM_RS00095"
                     /old_locus_tag="pgaptmp_000019"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HGU4556633.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18004..20286)
                     /gene="fiu"
                     /locus_tag="ACHOSM_RS00100"
                     /old_locus_tag="pgaptmp_000020"
     CDS             complement(18004..20286)
                     /gene="fiu"
                     /locus_tag="ACHOSM_RS00100"
                     /old_locus_tag="pgaptmp_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HGU4556634.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNSTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPVGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(20551..20811)
                     /gene="mcbA"
                     /locus_tag="ACHOSM_RS00105"
                     /old_locus_tag="pgaptmp_000021"
     CDS             complement(20551..20811)
                     /gene="mcbA"
                     /locus_tag="ACHOSM_RS00105"
                     /old_locus_tag="pgaptmp_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HGU4556635.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            21087..22013
                     /gene="rlmF"
                     /locus_tag="ACHOSM_RS00110"
                     /old_locus_tag="pgaptmp_000022"
     CDS             21087..22013
                     /gene="rlmF"
                     /locus_tag="ACHOSM_RS00110"
                     /old_locus_tag="pgaptmp_000022"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HGU4556636.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(22010..24235)
                     /gene="ybiO"
                     /locus_tag="ACHOSM_RS00115"
                     /old_locus_tag="pgaptmp_000023"
     CDS             complement(22010..24235)
                     /gene="ybiO"
                     /locus_tag="ACHOSM_RS00115"
                     /old_locus_tag="pgaptmp_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HGU4556637.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGLWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(24352..25074)
                     /gene="glnQ"
                     /locus_tag="ACHOSM_RS00120"
                     /old_locus_tag="pgaptmp_000024"
     CDS             complement(24352..25074)
                     /gene="glnQ"
                     /locus_tag="ACHOSM_RS00120"
                     /old_locus_tag="pgaptmp_000024"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HGU4556638.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(25071..25730)
                     /gene="glnP"
                     /locus_tag="ACHOSM_RS00125"
                     /old_locus_tag="pgaptmp_000025"
     CDS             complement(25071..25730)
                     /gene="glnP"
                     /locus_tag="ACHOSM_RS00125"
                     /old_locus_tag="pgaptmp_000025"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HGU4556639.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(25869..26615)
                     /gene="glnH"
                     /locus_tag="ACHOSM_RS00130"
                     /old_locus_tag="pgaptmp_000026"
     CDS             complement(25869..26615)
                     /gene="glnH"
                     /locus_tag="ACHOSM_RS00130"
                     /old_locus_tag="pgaptmp_000026"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HGU4556640.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(27019..27522)
                     /gene="dps"
                     /locus_tag="ACHOSM_RS00135"
                     /old_locus_tag="pgaptmp_000027"
                     /gene_synonym="pexB"
     CDS             complement(27019..27522)
                     /gene="dps"
                     /locus_tag="ACHOSM_RS00135"
                     /old_locus_tag="pgaptmp_000027"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HGU4556641.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(27821..28708)
                     /gene="rhtA"
                     /locus_tag="ACHOSM_RS00140"
                     /old_locus_tag="pgaptmp_000028"
     CDS             complement(27821..28708)
                     /gene="rhtA"
                     /locus_tag="ACHOSM_RS00140"
                     /old_locus_tag="pgaptmp_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HGU4556642.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGIT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            28943..29008
                     /locus_tag="ACHOSM_RS00145"
                     /old_locus_tag="pgaptmp_000029"
     CDS             28943..29008
                     /locus_tag="ACHOSM_RS00145"
                     /old_locus_tag="pgaptmp_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4556643.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            29061..29576
                     /gene="ompX"
                     /locus_tag="ACHOSM_RS00150"
                     /old_locus_tag="pgaptmp_000030"
     CDS             29061..29576
                     /gene="ompX"
                     /locus_tag="ACHOSM_RS00150"
                     /old_locus_tag="pgaptmp_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HGU4556644.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(29625..31208)
                     /gene="opgE"
                     /locus_tag="ACHOSM_RS00155"
                     /old_locus_tag="pgaptmp_000031"
     CDS             complement(29625..31208)
                     /gene="opgE"
                     /locus_tag="ACHOSM_RS00155"
                     /old_locus_tag="pgaptmp_000031"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGU4556645.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(31480..31608)
                     /gene="mntS"
                     /locus_tag="ACHOSM_RS00160"
                     /old_locus_tag="pgaptmp_000032"
     CDS             complement(31480..31608)
                     /gene="mntS"
                     /locus_tag="ACHOSM_RS00160"
                     /old_locus_tag="pgaptmp_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HGU4556646.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            31794..32261
                     /gene="mntR"
                     /locus_tag="ACHOSM_RS00165"
                     /old_locus_tag="pgaptmp_000033"
     CDS             31794..32261
                     /gene="mntR"
                     /locus_tag="ACHOSM_RS00165"
                     /old_locus_tag="pgaptmp_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HGU4556647.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            32258..33376
                     /gene="ybiR"
                     /locus_tag="ACHOSM_RS00170"
                     /old_locus_tag="pgaptmp_000034"
     CDS             32258..33376
                     /gene="ybiR"
                     /locus_tag="ACHOSM_RS00170"
                     /old_locus_tag="pgaptmp_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HGU4556648.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(33435..34355)
                     /gene="ldtB"
                     /locus_tag="ACHOSM_RS00175"
                     /old_locus_tag="pgaptmp_000035"
     CDS             complement(33435..34355)
                     /gene="ldtB"
                     /locus_tag="ACHOSM_RS00175"
                     /old_locus_tag="pgaptmp_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4556649.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            34574..36166
                     /gene="ybiT"
                     /locus_tag="ACHOSM_RS00180"
                     /old_locus_tag="pgaptmp_000036"
     CDS             34574..36166
                     /gene="ybiT"
                     /locus_tag="ACHOSM_RS00180"
                     /old_locus_tag="pgaptmp_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HGU4556650.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDGEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(36407..37672)
                     /gene="ybiU"
                     /locus_tag="ACHOSM_RS00185"
                     /old_locus_tag="pgaptmp_000037"
     CDS             complement(36407..37672)
                     /gene="ybiU"
                     /locus_tag="ACHOSM_RS00185"
                     /old_locus_tag="pgaptmp_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HGU4556651.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(37824..38639)
                     /gene="ybiV"
                     /locus_tag="ACHOSM_RS00190"
                     /old_locus_tag="pgaptmp_000038"
     CDS             complement(37824..38639)
                     /gene="ybiV"
                     /locus_tag="ACHOSM_RS00190"
                     /old_locus_tag="pgaptmp_000038"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HGU4556652.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDYVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(38785..41217)
                     /gene="ybiW"
                     /locus_tag="ACHOSM_RS00195"
                     /old_locus_tag="pgaptmp_000039"
     CDS             complement(38785..41217)
                     /gene="ybiW"
                     /locus_tag="ACHOSM_RS00195"
                     /old_locus_tag="pgaptmp_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HGU4556653.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            complement(41223..42122)
                     /gene="ybiY"
                     /locus_tag="ACHOSM_RS00200"
                     /old_locus_tag="pgaptmp_000040"
     CDS             complement(41223..42122)
                     /gene="ybiY"
                     /locus_tag="ACHOSM_RS00200"
                     /old_locus_tag="pgaptmp_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGU4556654.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            42253..42915
                     /gene="fsa"
                     /locus_tag="ACHOSM_RS00205"
                     /old_locus_tag="pgaptmp_000041"
     CDS             42253..42915
                     /gene="fsa"
                     /locus_tag="ACHOSM_RS00205"
                     /old_locus_tag="pgaptmp_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU4556655.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            complement(42991..43740)
                     /gene="moeB"
                     /locus_tag="ACHOSM_RS00210"
                     /old_locus_tag="pgaptmp_000042"
     CDS             complement(42991..43740)
                     /gene="moeB"
                     /locus_tag="ACHOSM_RS00210"
                     /old_locus_tag="pgaptmp_000042"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HGU4556656.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(43740..44975)
                     /gene="moeA"
                     /locus_tag="ACHOSM_RS00215"
                     /old_locus_tag="pgaptmp_000043"
     CDS             complement(43740..44975)
                     /gene="moeA"
                     /locus_tag="ACHOSM_RS00215"
                     /old_locus_tag="pgaptmp_000043"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HGU4556657.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            45179..46144
                     /gene="iaaA"
                     /locus_tag="ACHOSM_RS00220"
                     /old_locus_tag="pgaptmp_000044"
     CDS             45179..46144
                     /gene="iaaA"
                     /locus_tag="ACHOSM_RS00220"
                     /old_locus_tag="pgaptmp_000044"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGU4556658.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWCYAGDTPTTGIYREKGDTVATQ"
     gene            46131..48002
                     /gene="gsiA"
                     /locus_tag="ACHOSM_RS00225"
                     /old_locus_tag="pgaptmp_000045"
     CDS             46131..48002
                     /gene="gsiA"
                     /locus_tag="ACHOSM_RS00225"
                     /old_locus_tag="pgaptmp_000045"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HGU4556659.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            48022..49560
                     /gene="gsiB"
                     /locus_tag="ACHOSM_RS00230"
                     /old_locus_tag="pgaptmp_000046"
     CDS             48022..49560
                     /gene="gsiB"
                     /locus_tag="ACHOSM_RS00230"
                     /old_locus_tag="pgaptmp_000046"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HGU4556660.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVAPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQYIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            49578..50498
                     /gene="gsiC"
                     /locus_tag="ACHOSM_RS00235"
                     /old_locus_tag="pgaptmp_000047"
     CDS             49578..50498
                     /gene="gsiC"
                     /locus_tag="ACHOSM_RS00235"
                     /old_locus_tag="pgaptmp_000047"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HGU4556661.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVVSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            50501..51412
                     /gene="gsiD"
                     /locus_tag="ACHOSM_RS00240"
                     /old_locus_tag="pgaptmp_000048"
     CDS             50501..51412
                     /gene="gsiD"
                     /locus_tag="ACHOSM_RS00240"
                     /old_locus_tag="pgaptmp_000048"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HGU4556662.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            51590..53938
                     /gene="pdeI"
                     /locus_tag="ACHOSM_RS00245"
                     /old_locus_tag="pgaptmp_000049"
     CDS             51590..53938
                     /gene="pdeI"
                     /locus_tag="ACHOSM_RS00245"
                     /old_locus_tag="pgaptmp_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4556663.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGSLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            53946..55274
                     /gene="dgcI"
                     /locus_tag="ACHOSM_RS00250"
                     /old_locus_tag="pgaptmp_000050"
                     /pseudo
     CDS             53946..55274
                     /gene="dgcI"
                     /locus_tag="ACHOSM_RS00250"
                     /old_locus_tag="pgaptmp_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(55321..56646)
                     /gene="rimO"
                     /locus_tag="ACHOSM_RS00255"
                     /old_locus_tag="pgaptmp_000051"
     CDS             complement(55321..56646)
                     /gene="rimO"
                     /locus_tag="ACHOSM_RS00255"
                     /old_locus_tag="pgaptmp_000051"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HGU4556664.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            56859..57242
                     /gene="bssR"
                     /locus_tag="ACHOSM_RS00260"
                     /old_locus_tag="pgaptmp_000052"
     CDS             56859..57242
                     /gene="bssR"
                     /locus_tag="ACHOSM_RS00260"
                     /old_locus_tag="pgaptmp_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HGU4556665.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            57353..58468
                     /gene="yliI"
                     /locus_tag="ACHOSM_RS00265"
                     /old_locus_tag="pgaptmp_000053"
     CDS             57353..58468
                     /gene="yliI"
                     /locus_tag="ACHOSM_RS00265"
                     /old_locus_tag="pgaptmp_000053"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HGU4556666.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FVALGENNQRSMAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(58465..59091)
                     /gene="gstB"
                     /locus_tag="ACHOSM_RS00270"
                     /old_locus_tag="pgaptmp_000054"
     CDS             complement(58465..59091)
                     /gene="gstB"
                     /locus_tag="ACHOSM_RS00270"
                     /old_locus_tag="pgaptmp_000054"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HGU4556667.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            59337..60539
                     /gene="dacC"
                     /locus_tag="ACHOSM_RS00275"
                     /old_locus_tag="pgaptmp_000055"
     CDS             59337..60539
                     /gene="dacC"
                     /locus_tag="ACHOSM_RS00275"
                     /old_locus_tag="pgaptmp_000055"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU4556668.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIALMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(60586..61344)
                     /gene="deoR"
                     /locus_tag="ACHOSM_RS00280"
                     /old_locus_tag="pgaptmp_000056"
     CDS             complement(60586..61344)
                     /gene="deoR"
                     /locus_tag="ACHOSM_RS00280"
                     /old_locus_tag="pgaptmp_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HGU4556669.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(61402..61998)
                     /gene="ybjG"
                     /locus_tag="ACHOSM_RS00285"
                     /old_locus_tag="pgaptmp_000057"
     CDS             complement(61402..61998)
                     /gene="ybjG"
                     /locus_tag="ACHOSM_RS00285"
                     /old_locus_tag="pgaptmp_000057"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU4556670.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            62283..63515
                     /gene="mdfA"
                     /locus_tag="ACHOSM_RS00290"
                     /old_locus_tag="pgaptmp_000058"
     CDS             62283..63515
                     /gene="mdfA"
                     /locus_tag="ACHOSM_RS00290"
                     /old_locus_tag="pgaptmp_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HGU4556671.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(63555..63839)
                     /gene="ybjH"
                     /locus_tag="ACHOSM_RS00295"
                     /old_locus_tag="pgaptmp_000059"
     CDS             complement(63555..63839)
                     /gene="ybjH"
                     /locus_tag="ACHOSM_RS00295"
                     /old_locus_tag="pgaptmp_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HGU4556672.1"
                     /translation="MFMKNCLLLGALLMGFSGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(63925..64740)
                     /gene="ybjI"
                     /locus_tag="ACHOSM_RS00300"
                     /old_locus_tag="pgaptmp_000060"
     CDS             complement(63925..64740)
                     /gene="ybjI"
                     /locus_tag="ACHOSM_RS00300"
                     /old_locus_tag="pgaptmp_000060"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HGU4556673.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(64740..65947)
                     /gene="ybjJ"
                     /locus_tag="ACHOSM_RS00305"
                     /old_locus_tag="pgaptmp_000061"
                     /pseudo
     CDS             complement(64740..65947)
                     /gene="ybjJ"
                     /locus_tag="ACHOSM_RS00305"
                     /old_locus_tag="pgaptmp_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            66031..66567
                     /gene="rcdA"
                     /locus_tag="ACHOSM_RS00310"
                     /old_locus_tag="pgaptmp_000062"
     CDS             66031..66567
                     /gene="rcdA"
                     /locus_tag="ACHOSM_RS00310"
                     /old_locus_tag="pgaptmp_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HGU4556674.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSLQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLLREEILRMVERVAG"
     gene            complement(66742..68427)
                     /gene="ybjL"
                     /locus_tag="ACHOSM_RS00315"
                     /old_locus_tag="pgaptmp_000063"
     CDS             complement(66742..68427)
                     /gene="ybjL"
                     /locus_tag="ACHOSM_RS00315"
                     /old_locus_tag="pgaptmp_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HGU4556675.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            68697..69074
                     /gene="ybjM"
                     /locus_tag="ACHOSM_RS00320"
                     /old_locus_tag="pgaptmp_000064"
     CDS             68697..69074
                     /gene="ybjM"
                     /locus_tag="ACHOSM_RS00320"
                     /old_locus_tag="pgaptmp_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HGU4556676.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(69104..69361)
                     /gene="grxA"
                     /locus_tag="ACHOSM_RS00325"
                     /old_locus_tag="pgaptmp_000065"
     CDS             complement(69104..69361)
                     /gene="grxA"
                     /locus_tag="ACHOSM_RS00325"
                     /old_locus_tag="pgaptmp_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HGU4556677.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            69521..69808
                     /gene="ybjC"
                     /locus_tag="ACHOSM_RS00330"
                     /old_locus_tag="pgaptmp_000066"
     CDS             69521..69808
                     /gene="ybjC"
                     /locus_tag="ACHOSM_RS00330"
                     /old_locus_tag="pgaptmp_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HGU4556678.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            69792..70514
                     /gene="nfsA"
                     /locus_tag="ACHOSM_RS00335"
                     /old_locus_tag="pgaptmp_000067"
     CDS             69792..70514
                     /gene="nfsA"
                     /locus_tag="ACHOSM_RS00335"
                     /old_locus_tag="pgaptmp_000067"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HGU4556679.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            70575..71477
                     /gene="rimK"
                     /locus_tag="ACHOSM_RS00340"
                     /old_locus_tag="pgaptmp_000068"
     CDS             70575..71477
                     /gene="rimK"
                     /locus_tag="ACHOSM_RS00340"
                     /old_locus_tag="pgaptmp_000068"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HGU4556680.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            71565..72041
                     /gene="ybjN"
                     /locus_tag="ACHOSM_RS00345"
                     /old_locus_tag="pgaptmp_000069"
     CDS             71565..72041
                     /gene="ybjN"
                     /locus_tag="ACHOSM_RS00345"
                     /old_locus_tag="pgaptmp_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HGU4556681.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSISVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            72392..73504
                     /gene="potF"
                     /locus_tag="ACHOSM_RS00350"
                     /old_locus_tag="pgaptmp_000070"
     CDS             72392..73504
                     /gene="potF"
                     /locus_tag="ACHOSM_RS00350"
                     /old_locus_tag="pgaptmp_000070"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HGU4556682.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            73599..74732
                     /gene="potG"
                     /locus_tag="ACHOSM_RS00355"
                     /old_locus_tag="pgaptmp_000071"
     CDS             73599..74732
                     /gene="potG"
                     /locus_tag="ACHOSM_RS00355"
                     /old_locus_tag="pgaptmp_000071"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HGU4556683.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDPDASVVDNVPVHVALRPEKIMLCEEPPADGYNFAVGEVMHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRYRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            74742..75695
                     /gene="potH"
                     /locus_tag="ACHOSM_RS00360"
                     /old_locus_tag="pgaptmp_000072"
     CDS             74742..75695
                     /gene="potH"
                     /locus_tag="ACHOSM_RS00360"
                     /old_locus_tag="pgaptmp_000072"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HGU4556684.1"
                     /translation="MSTLEPAAQPKPPGGLRLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQSLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDSSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            75692..76537
                     /gene="potI"
                     /locus_tag="ACHOSM_RS00365"
                     /old_locus_tag="pgaptmp_000073"
     CDS             75692..76537
                     /gene="potI"
                     /locus_tag="ACHOSM_RS00365"
                     /old_locus_tag="pgaptmp_000073"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HGU4556685.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            76597..77085
                     /gene="ybjO"
                     /locus_tag="ACHOSM_RS00370"
                     /old_locus_tag="pgaptmp_000074"
     CDS             76597..77085
                     /gene="ybjO"
                     /locus_tag="ACHOSM_RS00370"
                     /old_locus_tag="pgaptmp_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HGU4556686.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            77126..78253
                     /gene="rlmC"
                     /locus_tag="ACHOSM_RS00375"
                     /old_locus_tag="pgaptmp_000075"
     CDS             77126..78253
                     /gene="rlmC"
                     /locus_tag="ACHOSM_RS00375"
                     /old_locus_tag="pgaptmp_000075"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HGU4556687.1"
                     /translation="MQCALYDAGRCHSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(78428..79159)
                     /gene="artJ"
                     /locus_tag="ACHOSM_RS00380"
                     /old_locus_tag="pgaptmp_000076"
     CDS             complement(78428..79159)
                     /gene="artJ"
                     /locus_tag="ACHOSM_RS00380"
                     /old_locus_tag="pgaptmp_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HGU4556688.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(79451..80119)
                     /gene="artM"
                     /locus_tag="ACHOSM_RS00385"
                     /old_locus_tag="pgaptmp_000077"
     CDS             complement(79451..80119)
                     /gene="artM"
                     /locus_tag="ACHOSM_RS00385"
                     /old_locus_tag="pgaptmp_000077"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HGU4556689.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(80119..80835)
                     /gene="artQ"
                     /locus_tag="ACHOSM_RS00390"
                     /old_locus_tag="pgaptmp_000078"
     CDS             complement(80119..80835)
                     /gene="artQ"
                     /locus_tag="ACHOSM_RS00390"
                     /old_locus_tag="pgaptmp_000078"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HGU4556690.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFLRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(80842..81573)
                     /gene="artI"
                     /locus_tag="ACHOSM_RS00395"
                     /old_locus_tag="pgaptmp_000079"
     CDS             complement(80842..81573)
                     /gene="artI"
                     /locus_tag="ACHOSM_RS00395"
                     /old_locus_tag="pgaptmp_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HGU4556691.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQDNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(81591..82319)
                     /gene="artP"
                     /locus_tag="ACHOSM_RS00400"
                     /old_locus_tag="pgaptmp_000080"
     CDS             complement(81591..82319)
                     /gene="artP"
                     /locus_tag="ACHOSM_RS00400"
                     /old_locus_tag="pgaptmp_000080"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HGU4556692.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(82537..83052)
                     /gene="ybjP"
                     /locus_tag="ACHOSM_RS00405"
                     /old_locus_tag="pgaptmp_000081"
     CDS             complement(82537..83052)
                     /gene="ybjP"
                     /locus_tag="ACHOSM_RS00405"
                     /old_locus_tag="pgaptmp_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4556693.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            83178..83501
                     /gene="ybjQ"
                     /locus_tag="ACHOSM_RS00410"
                     /old_locus_tag="pgaptmp_000082"
     CDS             83178..83501
                     /gene="ybjQ"
                     /locus_tag="ACHOSM_RS00410"
                     /old_locus_tag="pgaptmp_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HGU4556694.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARTLGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            83498..84328
                     /gene="amiD"
                     /locus_tag="ACHOSM_RS00415"
                     /old_locus_tag="pgaptmp_000083"
     CDS             83498..84328
                     /gene="amiD"
                     /locus_tag="ACHOSM_RS00415"
                     /old_locus_tag="pgaptmp_000083"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HGU4556695.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(84325..85338)
                     /gene="ybjS"
                     /locus_tag="ACHOSM_RS00420"
                     /old_locus_tag="pgaptmp_000084"
     CDS             complement(84325..85338)
                     /gene="ybjS"
                     /locus_tag="ACHOSM_RS00420"
                     /old_locus_tag="pgaptmp_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU4556696.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(85437..86867)
                     /gene="ybjT"
                     /locus_tag="ACHOSM_RS00425"
                     /old_locus_tag="pgaptmp_000085"
     CDS             complement(85437..86867)
                     /gene="ybjT"
                     /locus_tag="ACHOSM_RS00425"
                     /old_locus_tag="pgaptmp_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HGU4556697.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGHDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(86878..87879)
                     /gene="ltaE"
                     /locus_tag="ACHOSM_RS00430"
                     /old_locus_tag="pgaptmp_000086"
     CDS             complement(86878..87879)
                     /gene="ltaE"
                     /locus_tag="ACHOSM_RS00430"
                     /old_locus_tag="pgaptmp_000086"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HGU4556698.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGTDVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(87916..89634)
                     /gene="poxB"
                     /locus_tag="ACHOSM_RS00435"
                     /old_locus_tag="pgaptmp_000087"
     CDS             complement(87916..89634)
                     /gene="poxB"
                     /locus_tag="ACHOSM_RS00435"
                     /old_locus_tag="pgaptmp_000087"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HGU4556699.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(89767..90735)
                     /gene="hcr"
                     /locus_tag="ACHOSM_RS00440"
                     /old_locus_tag="pgaptmp_000088"
     CDS             complement(89767..90735)
                     /gene="hcr"
                     /locus_tag="ACHOSM_RS00440"
                     /old_locus_tag="pgaptmp_000088"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HGU4556700.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNCPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(90747..92399)
                     /gene="hcp"
                     /locus_tag="ACHOSM_RS00445"
                     /old_locus_tag="pgaptmp_000089"
                     /gene_synonym="priS"
     CDS             complement(90747..92399)
                     /gene="hcp"
                     /locus_tag="ACHOSM_RS00445"
                     /old_locus_tag="pgaptmp_000089"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HGU4556701.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(92543..93442)
                     /gene="lysO"
                     /locus_tag="ACHOSM_RS00450"
                     /old_locus_tag="pgaptmp_000090"
     CDS             complement(92543..93442)
                     /gene="lysO"
                     /locus_tag="ACHOSM_RS00450"
                     /old_locus_tag="pgaptmp_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HGU4556702.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQATLKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(93937..94632)
                     /gene="aqpZ"
                     /locus_tag="ACHOSM_RS00455"
                     /old_locus_tag="pgaptmp_000091"
     CDS             complement(93937..94632)
                     /gene="aqpZ"
                     /locus_tag="ACHOSM_RS00455"
                     /old_locus_tag="pgaptmp_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HGU4556703.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            95058..96716
                     /gene="ybjD"
                     /locus_tag="ACHOSM_RS00460"
                     /old_locus_tag="pgaptmp_000092"
     CDS             95058..96716
                     /gene="ybjD"
                     /locus_tag="ACHOSM_RS00460"
                     /old_locus_tag="pgaptmp_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HGU4556704.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(96713..97669)
                     /gene="ybjX"
                     /locus_tag="ACHOSM_RS00465"
                     /old_locus_tag="pgaptmp_000093"
     CDS             complement(96713..97669)
                     /gene="ybjX"
                     /locus_tag="ACHOSM_RS00465"
                     /old_locus_tag="pgaptmp_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HGU4556705.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            97820..98935
                     /gene="macA"
                     /locus_tag="ACHOSM_RS00470"
                     /old_locus_tag="pgaptmp_000094"
     CDS             97820..98935
                     /gene="macA"
                     /locus_tag="ACHOSM_RS00470"
                     /old_locus_tag="pgaptmp_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HGU4556706.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            98932..100878
                     /gene="macB"
                     /locus_tag="ACHOSM_RS00475"
                     /old_locus_tag="pgaptmp_000095"
     CDS             98932..100878
                     /gene="macB"
                     /locus_tag="ACHOSM_RS00475"
                     /old_locus_tag="pgaptmp_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HGU4556707.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(100951..101175)
                     /gene="cspD"
                     /locus_tag="ACHOSM_RS00480"
                     /old_locus_tag="pgaptmp_000096"
     CDS             complement(100951..101175)
                     /gene="cspD"
                     /locus_tag="ACHOSM_RS00480"
                     /old_locus_tag="pgaptmp_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HGU4556708.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            101325..101399
                     /gene="yljB"
                     /locus_tag="ACHOSM_RS00485"
                     /old_locus_tag="pgaptmp_000097"
     CDS             101325..101399
                     /gene="yljB"
                     /locus_tag="ACHOSM_RS00485"
                     /old_locus_tag="pgaptmp_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HGU4556709.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            101498..101818
                     /gene="clpS"
                     /locus_tag="ACHOSM_RS00490"
                     /old_locus_tag="pgaptmp_000098"
     CDS             101498..101818
                     /gene="clpS"
                     /locus_tag="ACHOSM_RS00490"
                     /old_locus_tag="pgaptmp_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HGU4556710.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            101849..104125
                     /gene="clpA"
                     /locus_tag="ACHOSM_RS00495"
                     /old_locus_tag="pgaptmp_000099"
     CDS             101849..104125
                     /gene="clpA"
                     /locus_tag="ACHOSM_RS00495"
                     /old_locus_tag="pgaptmp_000099"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HGU4556711.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPDSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(104469..104556)
                     /locus_tag="ACHOSM_RS00500"
                     /old_locus_tag="pgaptmp_000100"
     tRNA            complement(104469..104556)
                     /locus_tag="ACHOSM_RS00500"
                     /old_locus_tag="pgaptmp_000100"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(104520..104522),aa:Ser,seq:gga)
     gene            complement(104810..105028)
                     /gene="infA"
                     /locus_tag="ACHOSM_RS00505"
                     /old_locus_tag="pgaptmp_000101"
     CDS             complement(104810..105028)
                     /gene="infA"
                     /locus_tag="ACHOSM_RS00505"
                     /old_locus_tag="pgaptmp_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGU4556712.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(105313..106017)
                     /gene="aat"
                     /locus_tag="ACHOSM_RS00510"
                     /old_locus_tag="pgaptmp_000102"
     CDS             complement(105313..106017)
                     /gene="aat"
                     /locus_tag="ACHOSM_RS00510"
                     /old_locus_tag="pgaptmp_000102"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HGU4556713.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDRKEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(106059..107780)
                     /gene="cydC"
                     /locus_tag="ACHOSM_RS00515"
                     /old_locus_tag="pgaptmp_000103"
     CDS             complement(106059..107780)
                     /gene="cydC"
                     /locus_tag="ACHOSM_RS00515"
                     /old_locus_tag="pgaptmp_000103"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HGU4556714.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(107781..109547)
                     /gene="cydD"
                     /locus_tag="ACHOSM_RS00520"
                     /old_locus_tag="pgaptmp_000104"
     CDS             complement(107781..109547)
                     /gene="cydD"
                     /locus_tag="ACHOSM_RS00520"
                     /old_locus_tag="pgaptmp_000104"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HGU4556715.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(109670..110635)
                     /gene="trxB"
                     /locus_tag="ACHOSM_RS00525"
                     /old_locus_tag="pgaptmp_000105"
     CDS             complement(109670..110635)
                     /gene="trxB"
                     /locus_tag="ACHOSM_RS00525"
                     /old_locus_tag="pgaptmp_000105"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGU4556716.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            111180..111674
                     /gene="lrp"
                     /locus_tag="ACHOSM_RS00530"
                     /old_locus_tag="pgaptmp_000106"
     CDS             111180..111674
                     /gene="lrp"
                     /locus_tag="ACHOSM_RS00530"
                     /old_locus_tag="pgaptmp_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HGU4556717.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            111809..115759
                     /gene="ftsK"
                     /locus_tag="ACHOSM_RS00535"
                     /old_locus_tag="pgaptmp_000107"
     CDS             111809..115759
                     /gene="ftsK"
                     /locus_tag="ACHOSM_RS00535"
                     /old_locus_tag="pgaptmp_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGU4556718.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAGAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVE
                     ARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEV
                     IPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMP
                     HLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVV
                     TDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADCMMRPIPDPYWKPGD
                     SMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRP
                     SVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRV
                     HGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQF
                     VTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            115914..116525
                     /gene="lolA"
                     /locus_tag="ACHOSM_RS00540"
                     /old_locus_tag="pgaptmp_000108"
     CDS             115914..116525
                     /gene="lolA"
                     /locus_tag="ACHOSM_RS00540"
                     /old_locus_tag="pgaptmp_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HGU4556719.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            116536..117879
                     /gene="rarA"
                     /locus_tag="ACHOSM_RS00545"
                     /old_locus_tag="pgaptmp_000109"
                     /gene_synonym="mgsA"
     CDS             116536..117879
                     /gene="rarA"
                     /locus_tag="ACHOSM_RS00545"
                     /old_locus_tag="pgaptmp_000109"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HGU4556720.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAKVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPREIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            117970..119262
                     /gene="serS"
                     /locus_tag="ACHOSM_RS00550"
                     /old_locus_tag="pgaptmp_000110"
     CDS             117970..119262
                     /gene="serS"
                     /locus_tag="ACHOSM_RS00550"
                     /old_locus_tag="pgaptmp_000110"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGU4556721.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            119501..121945
                     /gene="dmsA"
                     /locus_tag="ACHOSM_RS00555"
                     /old_locus_tag="pgaptmp_000111"
     CDS             119501..121945
                     /gene="dmsA"
                     /locus_tag="ACHOSM_RS00555"
                     /old_locus_tag="pgaptmp_000111"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HGU4556722.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNASMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTLSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            121956..122573
                     /gene="dmsB"
                     /locus_tag="ACHOSM_RS00560"
                     /old_locus_tag="pgaptmp_000112"
     CDS             121956..122573
                     /gene="dmsB"
                     /locus_tag="ACHOSM_RS00560"
                     /old_locus_tag="pgaptmp_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HGU4556723.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            122575..123438
                     /gene="dmsC"
                     /locus_tag="ACHOSM_RS00565"
                     /old_locus_tag="pgaptmp_000113"
     CDS             122575..123438
                     /gene="dmsC"
                     /locus_tag="ACHOSM_RS00565"
                     /old_locus_tag="pgaptmp_000113"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HGU4556724.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISLLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(123473..124099)
                     /gene="ycaC"
                     /locus_tag="ACHOSM_RS00570"
                     /old_locus_tag="pgaptmp_000114"
     CDS             complement(123473..124099)
                     /gene="ycaC"
                     /locus_tag="ACHOSM_RS00570"
                     /old_locus_tag="pgaptmp_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HGU4556725.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            124413..125561
                     /gene="ycaD"
                     /locus_tag="ACHOSM_RS00575"
                     /old_locus_tag="pgaptmp_000115"
     CDS             124413..125561
                     /gene="ycaD"
                     /locus_tag="ACHOSM_RS00575"
                     /old_locus_tag="pgaptmp_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4556726.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            125771..127201
                     /gene="ycaM"
                     /locus_tag="ACHOSM_RS00580"
                     /old_locus_tag="pgaptmp_000116"
     CDS             125771..127201
                     /gene="ycaM"
                     /locus_tag="ACHOSM_RS00580"
                     /old_locus_tag="pgaptmp_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU4556727.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKSLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(127202..128110)
                     /gene="ycaN"
                     /locus_tag="ACHOSM_RS00585"
                     /old_locus_tag="pgaptmp_000117"
                     /pseudo
     CDS             complement(127202..128110)
                     /gene="ycaN"
                     /locus_tag="ACHOSM_RS00585"
                     /old_locus_tag="pgaptmp_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            128210..128800
                     /gene="ycaK"
                     /locus_tag="ACHOSM_RS00590"
                     /old_locus_tag="pgaptmp_000118"
     CDS             128210..128800
                     /gene="ycaK"
                     /locus_tag="ACHOSM_RS00590"
                     /old_locus_tag="pgaptmp_000118"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HGU4556728.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGQKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLINMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            complement(128882..129622)
                     /gene="pflA"
                     /locus_tag="ACHOSM_RS00595"
                     /old_locus_tag="pgaptmp_000119"
     CDS             complement(128882..129622)
                     /gene="pflA"
                     /locus_tag="ACHOSM_RS00595"
                     /old_locus_tag="pgaptmp_000119"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HGU4556729.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(129814..132096)
                     /gene="pflB"
                     /locus_tag="ACHOSM_RS00600"
                     /old_locus_tag="pgaptmp_000120"
     CDS             complement(129814..132096)
                     /gene="pflB"
                     /locus_tag="ACHOSM_RS00600"
                     /old_locus_tag="pgaptmp_000120"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU4556730.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQTDLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(132151..133008)
                     /gene="focA"
                     /locus_tag="ACHOSM_RS00605"
                     /old_locus_tag="pgaptmp_000121"
     CDS             complement(132151..133008)
                     /gene="focA"
                     /locus_tag="ACHOSM_RS00605"
                     /old_locus_tag="pgaptmp_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HGU4556731.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVLMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT        32300 a        33205 c        35262 g        32344 t
ORIGIN      
        1 gaatgcggca ttaatgagtg aacggcccat ttgggtaata agttgcttaa tgattgtgtt
       61 atggccaggc attatctcgc atggacgaaa taagctcctt gaactggcag gaaaatgcca
      121 tagtgaagag ttgtggagtc cggttcgttg cgcttttaca ggtgcattaa cactctatta
      181 ctattttatc ggcttctttg ggatacttgc cgcgatagct ataacgcttg tctggcaaag
      241 gcatacgcgg ttttttcatt agtcgtaaat gcacatacta aagaacttaa ccgaacttca
      301 catcgccgct tcatttttcg cccgcagctt cttcgcctgg ctttccagta acaggtaaca
      361 gatcagcgcc agcagcagtg ggataaagta atacagcaca cgataagcga gtagggcggc
      421 gataattgtg cctttagatg tatgctcccc tgccagtagc gcgataaaca ccgcttccag
      481 cacaccaatt cccgccggaa tatgcacgat gacaccagca atactactaa ccagcaacac
      541 gcccagtaca aagaaatagt tctcgctttg accaagtaac agccagataa tcgcccccat
      601 taccatccag ttaacgctgg aaatcagcat ctgggcaagg gcgaatttcc atgagggcag
      661 caccagcttt tgccctttga tggtcatatg gcggtgcttc gcgaaagcgc aaaaccacaa
      721 ataaaccgcg ataatcatca gtaagccaat gccgagaata cgcagcgtag tttgatcgac
      781 ataccagtga tccggcaact ccaccacgcc tgcggtaaag ataatccctg ccagtaaaat
      841 atagcccagc cagttggtgg taatactgag cgagaaaatc cgcgtaatag tgctgcccgg
      901 taaccctagt cgggagtaca aacgatagcg cataccaatg ccgccgaccc aggtactgag
      961 cgtcaggttg aaggcgtagc agataaacga caccagcatc acctggcgct tcgccagttt
     1021 gtgaccgcag taaaagcggg cgagcaggtc atagcagccg taaatcagat agctgacgac
     1081 caccagcccg accgcactaa gcagcgcaac gcgattgtag tcgcggatga ccttccagac
     1141 ctcttcccag tccacttttt tggcgtagac caccagcaac acaatcaccg cgataaaaaa
     1201 cagccaggtg aggatcttct ttgctaagcg ccagcgcggg tgtgatttac tcatcagggt
     1261 tttacccccg tgttttcagt ttctacccga tcctgcgttt ccattgtcgg ttgtgcgggg
     1321 ggatcaacct gcgtcagacg tggtgtgtgt gccggaagcc agccaaccag cgccgggaag
     1381 tggcgtaaaa agtggaacgc cagcacgctt ttggtcaggt tccaccaggt gcgtttcggc
     1441 agcatggtct catcgacctg ctgacaatct gcggcaataa tgccgttcag attatcgcgc
     1501 agcgtctggt taaaatgacg atcgtggatg atgacatttg cttcgagatt cagtgacaaa
     1561 ctgagcggat cgagattact ggaccctact gtcgcccagt gatcgtccat caatgccact
     1621 ttgccgtgga gcgggcggcg gcggtactca aatacctgaa cgccgccttt aaccagatag
     1681 ttatacagca agcgcgcacc gactctgaca atcggcatat ccggttcgcc ctgaatgatc
     1741 agtttgatcc gcaccccgcg ccgtgccgct ttacgcaagg cgtgtaaaaa tcgatagccg
     1801 gggaagaagt aggcgttggc gataatgact tctcgccgcg cctgagtgag cattttcaga
     1861 tagtgacgtt caatatcatc gcgatgttct tcgttatcgc gccagaccag caatacctgc
     1921 gcttctcccg gctggcggtt ctcttccgct ttgtgatgac gtcgccacca gcgtcgtgcc
     1981 gcgctctgtc taggcaggtt ttccagctca aactggagaa tatcttcgac aatcggccct
     2041 tcaaggcgta ccgcgtaatc ctgtttagcc tctggaccgt agctggacat atgctcggcg
     2101 gagtaattca gcccgccaat aaaggcgata cgcgcgtcga tcaccacaat tttgcgatgc
     2161 atccggcgaa acacattggt gcgcatacca aaaaggcgag ggcggggatc gtagtagcgg
     2221 aacactacgc cagctgccgt cagttcattg acaaactcat cgctgagatc cggcgaaccg
     2281 tagccatcca gcaagacttc cgctttaacc ccgcgttgcg ctgctgccag tagtgccgca
     2341 tgcagctgtt tgccgacgtt atcctcaaac cagataaacg tttcaagaat gatgcgttct
     2401 tgtgcctcgc caatcgcctt aaacaccgcg ggataatatt gctcgccgtt ttccagcaac
     2461 tggatcttat tgccttcgcg ccagctacat ttcataaatg aatctccgca cttaaagggg
     2521 catgatcaga aaggtgtcgc catgtccgca gcggcaacgc ggttggcgcg ctggcgctgg
     2581 catttttgac gtagatcctg tccagtcgta gtagaggaaa ttgcaccgga aacgtgcgcg
     2641 ccgggcgtcc gtgggcgcgg gtaaaaatct catccagtcc ggcctgcact tttaacggat
     2701 gattagcttt ttgccgccag tcattgaaat cacccgccac caataccggt tcgccgtccg
     2761 gtagctcatt gacccattcg gcgagcatcg caagctgcgc ctgacggtgc gcctcacgca
     2821 ggcccagatg tacgcacatc acatgaatcg cttttccggt catcggcggc acaatgcggc
     2881 agtagagcac gccgcgcttt tccgcaccat cgaccgaaac atcgcgattc tcataatgtt
     2941 caatgggata acgcgacagt acggcgttgc cgtgatgccc ttccgggtat acggcattgc
     3001 gaccgtagac aaaatcgctc cacatagtgt cggcgagaaa ctcgtagtgc gaggtatcgg
     3061 gccagttttc cacatgcagc ggatgaactt cgtgcgcgcc catcacttcc tgcaggcaaa
     3121 caatatcggc gctgacggta cgcaccgcgt cgcgaagttc cggcaaaatg aagcgtcggt
     3181 taaacgcggt aaaacctttg tgaatgttga tggtgagcac cttgaacgaa aattgttgtg
     3241 tttgatcggg cataattttc ctgtctttgc ctcttatctc attgaaatag tgtagtcggc
     3301 gtcacaaaaa ggtgcggtct tacggaattt tccgtaaagt tcggtactct gagtaagtag
     3361 agataaattc ttcaggagag aagccatgaa gtggcaacaa cgtgttcgtg tcgcaacggg
     3421 tctaagttgc tggcagatta tgttgcattt actggtagtg gcgctgctgg tggtgggctg
     3481 gatgagtaag actctggttc acgtcggcgt gggattatgc gcactgtatt gtgtcacggt
     3541 agtgatgatg ttggtgtttc agcgccaccc cgagcaacgc tggcgtgagg tggcagacgt
     3601 gctggaagag ctgaccacga cctggtattt tggcgcagcg ctgattgtgc tgtggctgtt
     3661 gtcccgcgta ctggaaaaca actttttgct ggcaattgca gggctggcaa tccttgccgg
     3721 cccggcggta gtgtctttgc tggcgaaaga taagaagtta catcacctta cgtctaaaca
     3781 tcgcgtacgc cgctgaccct gtcgtggccg ttatcaccag tagcggccac aaactattcc
     3841 acacaatatc cagactcgca tccttcaaat aaatctgctt ggtaatgtcc gtaaagtggc
     3901 gaatagggtt aatccacgtc aggttttgca gccatactgg catgttttcc accggcgaaa
     3961 cgtaaccgga aaggagaatg gcgggcatca taaagacaaa cacgccgata aacgcctgct
     4021 gttgtgtcga gcagagtgat gaaatcaaca gaccgaatcc caccagcgat aaaccgtaaa
     4081 tcaccatcgt aaagtagaac agcgccagcg atccggcgaa ggggatttga tacgcccaga
     4141 taccaatcgc cagcacaatg gtggcctgga aggtggcgac aattaacgcc ggtacggctt
     4201 tgccgatgca gatctgccag gtggtgagcg gcgaaaccag tagctgatcg agcgtacctt
     4261 gttcacgttc gcgggcgacg gaaagtgaag tgacgatcat tacgccgata gtggtgatca
     4321 tggcgatcag tgacggcacc acaaaccatt tgtagtcgag attcgggtta taccagttgc
     4381 gtaccaccag ctcgctgttg ttaggtttcg gttttccttc cagcagctcc tgctgataat
     4441 ttttgacgat ctgttgcagg tagttggcgg caatttgcgc actgttggag ttacgcccgt
     4501 cgaggatcaa ctgcaacggc gcggtctgga aggtatccag tttgcgcgag aagtcagccg
     4561 ggaaacgcac caggagtaac gccttttgtg tgtcgatggt tgggcggatc tcctgtgggc
     4621 ttttcagcag cagcacatga gtaaaggcgc tggcgcgggc aaaacgttgg gtcagctcca
     4681 ccgaatgctc gccgttatct tcatcgtaga tggcgatggt ggcgttagtc acttccagcg
     4741 tggcggcgaa cgggaacaga atcacctgaa taagcacagg taaaatcaga atcgcgcggg
     4801 tttgcggttc gcgcagcagc gactgcaact ctttgcggat taacgtccat aagcgatgaa
     4861 acatgctctt ctccctaatc cagccgacgt ttggttttca gccacgtcag gccgataaac
     4921 atcaccgccg aagcgatcaa aaacagcacg tttaccacca gcaccactgg aatattcccg
     4981 gcgaggaaca ggctttgcag ggtgctgacg aaataacgag cgggaataat gtacgtcacc
     5041 gcgcggatca ccgcgggcat actgtcgatc tgaaaaataa agccggaaag cataatcgac
     5101 ggcagaaaag cggcgttcag ggcgacctga gcggcattga actggttgcg ggtaatcgtg
     5161 gaaatcagca gccccatccc cagggtactg agtaaaaaca ggctggagat aaaaaacaga
     5221 atcagcagcg acccgcgata cggcacgccg agaataaaca ctgacaccag catacacagc
     5281 aacatcgcca gcatcccgag aaagtaataa gggatcagct tacacagcag cagttccgtg
     5341 cgggtaatct ccgtagagag cagagcctcc atggtgccgc gttcccattc tcgcgccacc
     5401 accagcgagg tgagaatcgc gccgatgacc gtcatgataa tggtcaccgc accggggata
     5461 ataaagtgct ggctaatcgc cgccgggtta aaccagtagc gggtttgtac atcaataagc
     5521 ggttcaaaag tctgcccgtt gtcctccgct cgctgcattt gccagatctg ccagatccct
     5581 tcgacatacc cctgtacaaa gttagcggta ttcggctcac tgccgtcggt gatcacctga
     5641 atcggtgcgg tggcgttggc gcgctccatc tgttccgcaa aatccaccgg aataaccacc
     5701 agaccgcgaa ttttccccgc ctgcattttg gcgatcagtt cctgacggtt atcgctgatg
     5761 gtggcgtcga tgtagggcga accggtcatg gtgtgggtga aatccagcgc cgcttcgcta
     5821 cgctgttcca gtaaaatccc gacccgcagc ttgctggagt cgaggttaat gccgtaacca
     5881 aaaacaaaca gcagtagcag cgggatcact accgcaatca gccagctact cggatcgcga
     5941 acgagctgcc gcgtctcttt aacgcacagc gcccgtacgc gacgccagga caggatcggg
     6001 ttactcattg ctatgctcct tatcccagtc gtggatcaac tgaataaagg cttgctccat
     6061 ggtgggatcg ggttgctcgt cgttggccga ctgcgctttc aaatcatccg gcgtgccgct
     6121 ggcaattaat ttcccacggt acaccaggcc gatgcggtcg caatattccg cttcatccat
     6181 aaagtgggtg gtgaccatca ccgtgacgcc tttctctacc atgctgttga tgtgcagcca
     6241 aaattcacgg cgggtgaggg ggtcaacgcc ggaagtcggt tcgtcgagaa agagaatatc
     6301 cggttcatgc atcagcgaac aggccagcgc cagccgctgt ttaaaaccta atggcagttc
     6361 atcggtggcg tgggaggcaa tacttttcag gccaaatgcc tcgctcatac gggagatttt
     6421 ttcgttctgc gcccgaccgc gtaagccata cacaccagag aaaaagcgta aattctgttc
     6481 gaccgtcagg ttgccgtaga gcgaaaattt ttgcgccata tagccgagat gctggcgcgc
     6541 tttaccggaa ctctctttca gatccatccc cagcaccagc gcctggccgg aagtcggcac
     6601 cagcaaaccg cacatcatct taaaggtggt cgatttaccc gcgccgtttg gccccagcaa
     6661 accaaaaatc tccccacgtt taacggcaaa gttgacgtga tcggtggcgg caaaatcacc
     6721 aaatttcttg gtcagttctt tcgcttcgat caccgtctcg ccgggtgtgc cttctaccgt
     6781 atgcaatatt gcacccagcg gcgattctga ggttccggca ccgcccagca aatcaataaa
     6841 cgcatcttca aaacgcggcg tagtttcgtt gatgttgatt tccggcatcc cgtcggcatg
     6901 gcgaatatcg tctggtgtgg cctctttttt gaggatcaga cgtaccgatt tcccctgaat
     6961 catgccgtcg ctgacctgcg gcagtttcaa ggcgcgttgc aacagtttgc ggttgccctc
     7021 gtgtggactg gtcatcagaa agctgcgtcc ggccatggtt tgtgtcaggg cttttggttc
     7081 tccctgatac agcaactcgc cttcgttcat cagtaacacg tcacggcact gctcggcttc
     7141 gtcgagatac gaggtactcc agaggattaa catcccttcg cccgccagct catgcaccat
     7201 ctgccacagt tcgcgccgtg agatagggtc aacgccgacg ccgggttcat cgagcagcaa
     7261 cactttcggt tcgcccacca gggtacaggc caggccgagt ttttgtttca tcccaccgga
     7321 gagcttgccc gccaggcgtc cggtaaacgg cccaagagac gtaaactcca gcaggcgagc
     7381 aaaagtttgc ttacgtgcct cgccggtgac gctgcgcaaa tccgcgtaca gattgaggtt
     7441 ctccatcacc gtgagatctt catacagacc aaatttctgc ggcatatagc cgagcacggc
     7501 gtgcagcgcg ccgtcgtttt tgatcggatc aaagccaatc accgtggcac tgccgctgtc
     7561 gggtttcagt aatcccgcca acatccgcat cagcgtggtt ttacctgcac cgtccggccc
     7621 caccaacccc gtcacataac cggcgtgaat ggtacaatcg agcggcgcga cggcgggctt
     7681 gtccatgccc ggaaagcgtt tttccaggcc gttcagcgtg ataacggcat cattcatgtc
     7741 ctgcctcgtc accgaattgt accgtcactg gcattccctg gcgtaacgca tcatcggcgt
     7801 cggtcaccac aatacgcagg cgatagacga ggtcggtacg cagatccggt gtttcgacgg
     7861 ttttcggggt aaattcagca gtcggcgaaa cgaaaccaat ctgcccgtga tacggcttgt
     7921 ccgggcgacc atcggtataa agcagcactt tgcgccccgg ctgggcctgg tcaagattcc
     7981 gttcatcaac ataagcgcgc acccacaccg gacgcgttag tgaaacggta aacaccgtgc
     8041 caccttcatt gagaaccgtg cctggctcta ccgcgcgcgt taacagcgtg ccatcagacg
     8101 gtgctatcaa cgttgagtcc tgtaaattca gctccgcctg cgccagttgc gcctgcgcct
     8161 gttcgaggct ggctttcgcc tgagcgatgt cctgttcacg gttaccggaa cggtactgac
     8221 gcaatttatc ctgtgctgat ttcagcgttg cctgcgcctg gtcgcgcgag gagcgggcat
     8281 tttccaggtc atttgccgaa atagtgcggc ttttccacag tccttgctgg cggttataga
     8341 agttctgcgc atagtcatag gcggcttgcg cctgtttcac cgctgcggcg gcctgagcga
     8401 tttcttcatc gcgatacccg gcaagcatca gatcatactg cgcctgtgcc accgagacgc
     8461 ccgctttcgc ctgcatcagg gcaatctcat acggcttgtg atccagttcg cccagcacct
     8521 ggcccgcttt gatagcatca ccttcgtcca ccgccagcga ttcaacgcgc cccccaacac
     8581 ggaaactaag attcaccgta cgaatatcca cgttgccata cagcgtcagg ccgttatcct
     8641 ggcggctttg ataccaccag tagcctccgg caaccacggc ggcaagtacc actaccgcca
     8701 gtccgatcac gacaggtttt ttcatcacta caaactcctt tgcgataatc cttgcaaaat
     8761 caggtcgata tgacaggtca ccgtctggtt gatcagttcg gtcttttctt catcgaacgc
     8821 ggtccagccg gtacgtaaca gaattgtttc tttgccaaga cggaacgcca gaatctcacc
     8881 aatcaacgca tgggtatgaa ggatcatgcg ggtgtcattg gcgtcgctgc cggtccaggc
     8941 ggcaatcagg cgcgtcaggt ggctgtgtag cggactaatc acctgttcgt gcaccaggtg
     9001 gtaggctgcc gtgggagaga gctgctcacg ggagataaac ttgctgaggt tgacggtgtc
     9061 atcctgggtg agcagcttaa tcatgttcct gcaggcgcga aggatcagtt cacggatggc
     9121 agcccgatca ggctgtggtt gggcgaacaa gcgttcggct tcctcggcat gcggacggaa
     9181 ctgctcgcca ataaaatcgg caatccactg ggcgcaggcg aggtacaaat cttctttcga
     9241 accgaagtag taggtgatgg cagcgatatt ctgcccggcc tgggcggcta tctcgcgggt
     9301 ggtggcgttc attccatatt caccaaactg cgccagtgcg gcagcaatca gctgtttttt
     9361 cgcctgttca cccttgattg tcatggcagg attattcatc gcacagtcca ttcttaatca
     9421 aatgattgat taagattatg actccatatg ggagttgtcc agtatggcta agaattttag
     9481 ctacgccagt cacagggata atttatgcgc tgcgtcacaa aaactgctac actccgctcc
     9541 ctcatgacat tgtggttttt gtcattttcc ttttcagtat ctccctgaaa actacaccgg
     9601 taacggtcgg ggcggttcgg agtagttatg tctttcgatt ctttgggttt aagccctgat
     9661 atcctgcgcg ccgttgccga gcagggttac cgtgaaccca cccctattca gcagcaggcg
     9721 atccctgcgg tgctggaagg ccgcgacctg atggctagtg cccagaccgg caccggcaaa
     9781 acagcgggct ttacgctgcc gctgttgcaa cacctgatca ctcgccagcc gcacgccaaa
     9841 gggcgtcgtc cggtacgtgc gctcattctt accccgaccc gtgaactggc ggcacagatt
     9901 ggcgaaaacg tccgtgatta cagcaaatac ttgaacattc gttcgctggt ggtgtttggt
     9961 ggtgtgagta ttaacccgca gatgatgaaa ctgcgtggcg gcgttgatgt gctggtggca
    10021 acgccgggac gtttgctgga cctggaacat cagaatgcag tgaagctgga tcaggttgaa
    10081 atcctcgtcc tcgatgaagc tgaccgcatg ctcgacatgg gctttatcca cgatatccgt
    10141 cgcgtgttaa caaaactacc tgcgaagcgc cagaacctgt tattctccgc gaccttctct
    10201 gacgatatta aagccctggc ggaaaaactg ttgcacaacc cgctggaaat cgaagtggca
    10261 cgccgcaata ccgcgtctga tcaggtgacc cagcacgttc actttgtcga taagaaacgc
    10321 aaacgcgaat tgctgtcgca catgattggg aaagggaact ggcagcaggt gctggtgttt
    10381 acccgtacca aacacggcgc taaccatctg gctgaacagc tcaataaaga tggcatccgt
    10441 agtgcggcga tccacggcaa taaatcgcaa ggtgcgcgta ctcgtgcgct ggctgatttt
    10501 aaatcgggcg atattcgtgt actggtggca actgacatcg ctgcgcgcgg cctggatatt
    10561 gaagagctgc cgcacgtcgt caactatgaa ctgccaaacg tacctgaaga ttatgtccac
    10621 cgtatcgggc gtaccggtcg tgcggctgct accggtgaag cgttgtcgct ggtgtgtgtt
    10681 gatgaacaca aactgctgcg tgatatcgaa aaactgctga aaaaagagat cccgcgcatt
    10741 gcgattccgg gctatgagcc ggacccgtca atcaaagccg aaccgatcca gaacggtcgc
    10801 cagcaacgtg gcggcggcgg tcgtgggcaa ggtggtggtc gcggtcaaca gcaaccacgc
    10861 cgtggggaag gtggcgcaaa atctgcaagc gcgaaacctg cagaaaaacc gtctcgccgc
    10921 ctcggcgatg ccaaaccggc aggcgaacaa caacgtcgcc gccgtccgcg taaacctgcc
    10981 gctgcgcagt aatcttttat gccgggctat gcccggcatc aggctgatga acaaacgcaa
    11041 aactgcctga tgcgctacgc ttatcaggcc tacgtgaact ctgcaatata ttgaatttgc
    11101 atgcttttgt aggccggata aggcgtttac gccgcatccg gcatttcaca acaagcactt
    11161 gtcagcaatt tgagaacacg ggaaaaaatt tatctgtttt accgcccata agccaccgag
    11221 acgttacttc tctatagcca attgttcgcg caactccatt aaaaggtagc ccagtcgatt
    11281 cttgccctta ccattaccac cgtctcccca gtaagcatcg ttttccgtat gctcaaccag
    11341 ttttgcgggc gcggttgcca gcaagagcgc acgcagttct gcatgctgtt cgaatttagc
    11401 gcgaagtgct tttcgcatca cctgttcttt gaccgactcc cagtttttac gcagaggctt
    11461 agaacgatcg cgccccatgc gtgcggcgac cataggggaa gaaacccgac ggatctcttc
    11521 gcggtatttt tcatcaagga atttttgtgc ctgaaaatag tgttctgagg tgggctaggt
    11581 tttcccgtca accttgatgg gccaggcggc aaagttagaa aaatcaccgt agtcatcgct
    11641 ggtgctgtag aaatttatga tggtgtcttg catgacgtgt tggatccttt gtgctcgaac
    11701 gggcattaaa ccgcattatg ttggtggtta ttgcgagccg ctttccagaa acagaaaaac
    11761 cattacccct gaaaaccgaa aaatgccaca atattggctg tttatacagt atttcaggtt
    11821 ttctcatggc attaaccgcc gcgcttaaag cgcaaattgc cgcctggtat aaggcgcttc
    11881 aggaacagat ccccgacttt attccccgtg cgccgcagcg gcagatgatt gcggacgtcg
    11941 ccaaaacgct ggccggagaa gaagggcggc atctggcgat tgaagccccc accggcgttg
    12001 ggaaaacgct ctcctatttg atccccggca tcgccattgc ccgcgaagag caaaaaacgc
    12061 tggtggtgag taccgccaac gtggcgttgc aggatcagat ttacagcaaa gatttaccgc
    12121 tgctgaaaaa gatcattccc gatcttaaat tcactgccgc tttcgggcgt gggcgctacg
    12181 tttgtccgcg taatctgacg gcgctcgcca gcactgaacc cacgcaacag gatctgctgg
    12241 cgtttcttga cgacgaactg acgccaaata atcaggaaga gcaaaaacgt tgtgcgaagc
    12301 tgaagggcga tctcgacact tataaatggg atggtctgcg cgatcatacg gatatcgcta
    12361 tagatgatga tctctggcgt cgtttgagta ccgacaaagc cagctgcctc aaccgtaact
    12421 gttactacta tcgtgaatgc ccgttttttg ttgctcgtcg ggagattcag gaagcggaag
    12481 tggtggtggc aaaccatgcg ctggtgatgg cggcgatgga aagcgaagcc gtactgcctg
    12541 acccgaaaaa tctgctgctg gtgctggacg aaggtcatca cctgccagat gtggcccggg
    12601 atgcgctgga gatgagcgct gaaatcaccg cgccctggta tcgactacag ctggacttgt
    12661 tcacgaaact ggtcgctacc tgcatggagc agtttcgccc gaagaccatc ccaccgctgg
    12721 cgatccctga acgtttgaat gcgcattgtg aagagttgta tgagcttatc gcctcgttaa
    12781 acaacattct caatctctac atgcctgccg ggcaggaggc agagcaccgt tttgcgatgg
    12841 gcgaactgcc tgatgaagtg ctggagatct gccagcggct ggcaaaactc accgagatgc
    12901 tgcgtggtct ggcggagtta tttcttaacg atttaagtga gaaaaccggc agccatgaca
    12961 ttgtacgcct gcatcggttg attttgcaga tgaaccgcgc gttggggatg ttcgaggcgc
    13021 aaagcaaact ctggcggctg gcgtctctgg cgcaatcttc cggtgcgccg gtgaccaaat
    13081 gggcgacgcg ggaagagcgc gaagggcagc tgcatctctg gtttcactgc gtggggatac
    13141 gtgtcagcga tcagctggaa aggctgctgt ggcgcagtat tccgcacatt attgttacct
    13201 ccgcaacctt gcgttcgctg aatagttttt cgcgtttgca ggagatgagt ggtctgaaag
    13261 agaaagcggg cgaccgtttt gtggcgctgg attccccctt taaccactgc gaacagggca
    13321 aaattgttat tccccggatg cgcgttgagc cttccatcga caacgaagag cagcatattg
    13381 ccgaaatggc ggcctttttc cgtgagcagg tggagagcaa aaaacatctc ggtatgttgg
    13441 tactgtttgc cagcggacgg gcgatgcagc gctttctcga ctatgtgacg gatttacgcc
    13501 tgatgttgct ggtgcagggc gatcagccgc gttatcgctt agttgaactg caccgcaaac
    13561 gcgttgccaa cggtgagcgt agcgtgctgg tgggcttaca gtcatttgcc gaagggcttg
    13621 atttgaaagg tgatctgctc agccaggtgc atatccacaa aatcgctttt ccgcccatcg
    13681 acagccccgt ggtgatcacc gaaggggaat ggctgaaaag cctcaaccgc tatccgtttg
    13741 aggtgcaaag cctgccgagc gcctcgttta acctgattca gcaggttggg cgactgattc
    13801 gaagccacgg ttgctggggc gaagtggtta tctacgacaa acgcttgctg accaaaaatt
    13861 atggcaagcg actactggat gcattaccgg tatttccgat agagcaaccg gaagtccctg
    13921 aaggtatagt taaaaagaaa gaaaaaacga aatccccacg ccgtcggcgg cgttaatgat
    13981 gtgagtcagg taaggagtcg taaatggact atcgcaaaat cattaaagag atcgggcgcg
    14041 ggaaaaacca cgcgcgcgat ttagaccggg atactgcgcg cggtctgtat gctcatatgc
    14101 tcaatggtga agtccctgac cttgagttgg gcggcgtatt gattgcgctg cgtatcaaag
    14161 gggaagggga agcagagatg ctcggctttt acgaagccat gcaaaatcac accatcaagc
    14221 tgacgccgcc tgcaggcaag ccgatgccga ttgtcatccc cagttacaac ggcgcgcgta
    14281 aacaggccaa cctgacgccg ttgttggcga ttctgctgca caaactcggt tttcctgtgg
    14341 tggttcacgg ggttagcgaa gatccaaccc gcgtgctgac agaaaccatt ttcgaactga
    14401 tgggtattac gccaacgttg cacggcgggc aggcgcaggc gaagctcgac gaacatcaac
    14461 cggtgtttat gcctgtcggc gcgttttgcc cgccgctgga aaaacaactg gcgatgcgct
    14521 ggcggatggg cgtgcgtaac agtgcacata ccctggcgaa actggcgacg ccatttgccg
    14581 aaggtgaggc actgcgtctt tccagcgttt cgcatccgga atacattgga cgcgtcgcga
    14641 agttctttag cgatatcggc gggcgggcgc tactaatgca tggtactgaa ggtgaagtgt
    14701 atgctaatcc gcagcgctgc ccgcagatca atctcattga ccgtgaaggg atgcgggtgc
    14761 tgtatgaaaa acaggacact gcttgtagcg agttactgcc acaagcaaaa gatccggaaa
    14821 ccacggcgca gtggattgag cgttgcctgg ctggcagcga accgattccc gaatcgctga
    14881 aaatccagat ggcttgctgc ctggtggcta cgggtgaagc ggcaactatc agcgatggcc
    14941 tggagcgcgt taatcaggca ttttaatttt tatctcagcc ggatgagcca cgtcatccgg
    15001 cttttacccc accgcaatat gaaattcctg catctttatt gaccttcccg cgcccggcgt
    15061 gcagcataaa aatacaacaa acacataaca taaacaggag ttaaccatgg aaagtggtca
    15121 tcgctttgat gctcagacgc tgcacagttt tattcaggct gtatttcgtc agatgggtag
    15181 cgaggaacaa gaagcgaaat tagttgccga tcatttaatc gcggcaaacc tggcagggca
    15241 tgattctcat ggtattggca tgatcccaag ctatgtgcgt tcctggagtc aggggcatct
    15301 gcaaattaac catcatgcca aaatcgttaa agaggcgggg gcggcagtca cgctcgatgg
    15361 cgatcgcgca ttcggtcagg tcgcggcaca cgaagcgatg gcgctgggga ttgagaaagc
    15421 gcatcagcac ggtattgctg ccgtggcgct acataactcg catcatatcg gccgtatcgg
    15481 ttactgggcg gagcagtgtg cagcggcggg gtttgtctct atccactttg ttagcgtggt
    15541 cggtattcca atggtcgcgc cgttccacgg tcgcgacagc cgctttggca ccaatccctt
    15601 ctgtgtggtt ttccctcgta aagataattt cccgctgttg cttgattacg ccaccagcgc
    15661 cattgcattt ggcaaaaccc gcgtcgcctg gcataaaggc gtccccgtgc cgccaggttg
    15721 cctgattgac gttaacggcg tgccgacgac caatccggcg gtaatgcagg agtcgccgtt
    15781 gggttcgctg ttgacctttg ctgaacataa aggctacgcc cttgcagcga tgtgtgaaat
    15841 tcttggcggg gcgctttccg gcggtaaaac gacgcatcag gaaacgttac aaaccagtcc
    15901 cgatgccatt cttaactgca tgaccactat catcatcaac ccggaactct tcggcgcgcc
    15961 ggattgtagc gcgcagaccg aagcctttgc cgagtgggtg aaagcctcgc cgcatgatga
    16021 tgataagccg attttgctac cgggcgagtg ggaagtgaac acgcgtcgcg aacggcagga
    16081 gcaggggatt ccattggatg cgggaagctg gcaggccatt tgtgatgcgg cgcggcagat
    16141 tggtatgtcg gaagagacgt tgcagggttt ctgtcagcag ttagccagct aaaaaaagcc
    16201 cgtccagtaa cggacgggca aacaagggta atataggatc aatgagggtt agagcatatg
    16261 cgtctgtcgg caaacagaca gggaaatact tgtgctggac gtagcgtaaa cgcctgatcc
    16321 gtccaacggt tcggattttg taggcctgat aagacgcggt agcgtcgcat caggcatctg
    16381 ttgccggatg cggcgtaaac gccttatccg tcctacgaat tacttataaa ttaccgcagt
    16441 accgcttaat ttattgttgt tggtggcgga agtgatgctg tatccactgg caccggctgc
    16501 cgccgctttc tcagccagtt tcgcttccag ggcatcgagg gtggatgcgc catcggcaga
    16561 aaccacgccg attttattca tgttctgtgc ctgggatgcc gttaccggtt ccgcggcaaa
    16621 cacgccaaat gacagagttg aaagagccat agcagcaaca acagtattga tagttttcat
    16681 aattaatctc tcgcaggtga tttttgtata agtgacgttg tttcgtcgat gtgatgagta
    16741 tcacgttttt ttacgagaga taaaatcgaa gagaattgac cgccttgttc aaataaattg
    16801 attgatatct aacatactga taaaccattt atttgttgta cttatggact tgttttatgc
    16861 agcgtggcag tcacgacagc gttaaaggca cggaatgcca cgcaaaatac aaattatact
    16921 cgctatacat ctcgccagtt gtagttaacg taactggctg tctttcgaac ctctgcgatt
    16981 atatcctgta taagctggtt tttgtgaatc tttctcctgc tgacaatgaa tacataagcg
    17041 cacgccagga atggcttccc gacgggcttg cgggatgggg gcaccgcact cttcacattc
    17101 atacaggctt tcgccacgcg gaatttcacc ccgagcgcgg gcaatcgcat cttcaattgt
    17161 actgttgatc tgttcgttga cggcgtcatc gttagcccaa ccggatgcca tatcgtcctc
    17221 cccatatcaa tgcttgtaca gttaagtgta gctaatatag ggacgaactt gggcagttca
    17281 agcgtcagat ctccgaccat tcccgcagca gattatgata aagattgagc agcgacagga
    17341 tctcttcatt ttcaccatag cggcttttca gcgactgaat attgttgtcc agttcaaaca
    17401 gcatggcgcg ctttttatca tcgcggatca tcgactggat ccacataaat gatgccactc
    17461 gtacgccgcg ggttacgggt gtcacgcaat gcaggctgct ggagggatac aacacgagat
    17521 cacctgccgg gagttttacc cgatgttgtc cgaaggtgtc attaacgacc agttcgccgc
    17581 cgtcgtagct ttgtggatcg cttaaaaaca gcgtggcaga aaggtcagtg cgcatccagc
    17641 cgttttgtgg atggctgcgt actgcgccat ccacatgaaa accataggtt tcattgttct
    17701 gatagcgatt aaacagcggc gtggaaaggg tacgcggcaa ggccgcggca aagaataaag
    17761 catgttggtt aaccgcgttc agcacctcat tttgcagggc ggcgtataac gtgctgcggg
    17821 tgtctacctg ttgattgttc ttaacttgcg caccttgtgc gccggtggtg acgcgtccat
    17881 ccacccattc ggcttgttcc agttgttcgc gaaaacgagc gacgtcctgt ggcgataaca
    17941 cgccgggaat gtggtacatc atacaatttc tccaaaaagt ggggcctgcg ccccacatct
    18001 gaatcagaaa tgcatattgg ctgtgagcaa gaaggttctt ggctcgcccg ggtgataacg
    18061 gtagccgctc ttgttgattg aggcgacgta atcggtatca aacaggttgt aaacgtttag
    18121 ctggaagtcg agattgcgat taacccgata ccccagtttg gcatcggcga cccagtaacc
    18181 ttcggtaaac gctggcgttc ccaccgcgcc gtctgaacct ttatgcatac tgccgatata
    18241 gcgtgcgccc gcgccaacag agatatcatc ggttgcctga tactggctcc ataaggtgaa
    18301 ggcgtgctcc ggggtatacg gcagcgatga ggaaccatcc tgggcaacat ctttgccgtt
    18361 tttgatggtt gctttttgct gggtatagcc gccaatcacc tgccacgcgg gcgtgatatt
    18421 cccggccacg gatatctcat agccttcgac acgtttctta ccgtattgcg agtaagttcc
    18481 gtcatcattt tgctcaactt cattttcgat atcagtacgg aatagcgcgg cggtgagcaa
    18541 caggcgttta tccagaacct gccatttggt gccaatctcg ctggtgttgg ctttttgcgg
    18601 tttaaaatcg gtgcggttgg cactgttacc gctgccagac tgcgcaaggg cgaagttgtt
    18661 gccgcccgga ggctgctggg aaacggcata gttaatatag acattgccgt tttccgtcag
    18721 gtgatacagc gccccggctt tccagttcac cagattgccc gacttggcgg tgtcgacggt
    18781 ggtgaccgga gaaccttttg ccacaccaac tgggcaggtg atggcaccgc gtccgctgcc
    18841 gccgcaggcg gtggcactgt catattcagt atgataatta tccagacgga tcccgccgtt
    18901 caactcaaaa tcacgggtga tttgcagcgt atcgaaggcg taaattgcga aggtatccgt
    18961 ctgaccattg gcgtttgcgc cgttgcgcgt caggccgccg ggatgaatgc tgctgtcagg
    19021 atgataaata tttaccgccg gtaacgtcac cggattaacg ccgtagttag tctgcgtttc
    19081 acgggtaaat tccacgccgg tactgacatc atgaccgata gaaccggtat agaacgtcga
    19141 ggtcaggttg gtctggttgg tgagaatttt attactcaca tctttggtat tcgccgtgcg
    19201 tgaccaggtc cagctattca catcgctggt gggctgggta atattcgacg ccccacccat
    19261 aatcgccgtc atcaggtaat cctgcttcac gcgcgaccaa cgggtagtat tgcgaatggt
    19321 gctgttatca ttgatgtcgt gctcaaaacg catggtagcg gtgtcggtgg tcgaatcgtc
    19381 gtaatcggaa tccgtgccgt aaaagttatg cgtatcaact tttccggaat gattcagagc
    19441 cgctgttccc gcagatgggg cagaataacc cggcaaaccg atggtcggaa tgccgccgtc
    19501 tggcgtgttg tgctgggtga catgcagata attaagatac aaacgattcg ctgtaccaag
    19561 gccaaaagcg atagaagggg cgacgccgta acgctcattt ttgactttgt cgcgtccggc
    19621 atcgtgcgtt ttttcgccca tcacattcag gcgcaccgca gtggtatcac caatgacctg
    19681 attgacgtcc agcgtgccgc ggcggaacca ggcgctgcca atactggcgg aggcgtcaat
    19741 gccggaatca ttgcgcggct gcttgctgat catattgatc gagcccgtcg gcgcgctgcg
    19801 cccgtagtcg gtgccggaag gccctttaat cacttcgacc tgctcagtat tgaaggtgtc
    19861 gcgcgagacg ctgccgatat cgcgaatgcc atcaatataa atactgttag aggtatcagc
    19921 accgcgcata taaatggcgt cgccagtggt ggagttaccg ttctcacccg caaaaaacgc
    19981 acccacgccg gggacgtttt tgagcgcgtc ggtaaggttg gttgcgccct gatctttaat
    20041 cacttgttca gaaatcaccg tcatcgtgcg ggtagtatcc gctaccggac gcgagaattt
    20101 cggatcggca gattgttgtg gcgcataaag cgaaggcgtt gatgcttcga caaccagcgt
    20161 gtcatccgcg ttagtttgcc cttcggcggc gagtgcctga gccacaggcg tgatgccaat
    20221 acaaagaccg gcaaagaacg tgagcgaatg aaattgtctg gcagggaaat tgcgattgtt
    20281 ttccattttg caggtgactt ttttttatat gttgaaattt tcactgccac gagaaagcct
    20341 ctctgcggat gcgatgccac tgttaatctc gtgatagtgt tcgtgcaagt gataataatt
    20401 ttgataagca ttatcacttg gcgcgatttt ctatcaccca ttgcgcgaaa aagaaataca
    20461 tttatttaca ttgcgttcat atttgatgtt ttttgattac aaaacaggcg aaaactgcat
    20521 ccagaggcga atacagttac gttggtgggg ttatttgtag atggttgcag taccgaacat
    20581 ctggtcattt ccgcctgcgg aattaatgac ataacctttc gcgccttgct cgcgcgcttt
    20641 ttctgccagt ttatcctcca gatcgcttag gtttgatgcg ccagttgctg aaacggtacc
    20701 ggcgggccgt agctgaccgc tgtccaggtt actcatcggt tgcgcggcat aagccgtgag
    20761 agagattccg ctcaggacgg tggcaatcag tagtgtgagg cactttttca taatgacatc
    20821 ctcataaata caacgggtag tgcctgataa gtgtaggtcg cctgggcgat gaaaatgacg
    20881 caaaaaattg acagagatgt gcttattttt tgaacgatat atttttacaa aataagacaa
    20941 atcaatgaca tgcataatgc tgatttttat gatgaattgc cataaaccat agccatgtta
    21001 aggttttggg gaatgataaa cgcagccaga tatttaccct gtcgcgttat gcgggtatct
    21061 tacgccgctg cttaaaggag aatgctatgt ccgcccagaa accggggttg catccgcgca
    21121 accgtcatca cagccgctac gatctcgcca cgctttgtca ggtcaatcct gaactcaggc
    21181 aattcctcac gcttacaccc gcaggggagc aaagcgtaga ctttgccaat ccgctggcgg
    21241 tgaaggcgct caataaggcg ttgctggccc atttttacgc cgtagcgaac tgggatatcc
    21301 ccgacggttt tctctgccca ccagtaccgg gccgggcgga ttatattcat caccttgccg
    21361 atttactggc tgaagcaagc ggaacaattc cagcgaatgc cagcattctg gatatcggcg
    21421 ttggtgcgaa ctgtatttat ccgctgattg gcgtacatga atatggctgg cgttttaccg
    21481 gtagtgaaac cagcagccag gcgttaagca gtgcgcaggc gattatcagt gctaatccgg
    21541 ggcttaaccg cgccattcgt ctgcgtcggc aaaaagagag tggggcgatt tttaacggca
    21601 tcatccataa aaacgagcaa tacgacgcga ccttgtgtaa cccgccattc cacgattccg
    21661 ccgctgcagc gcgggcaggt agtgagcgta aacgccgtaa cctggggctg aacaaagacg
    21721 atgcactgaa ctttggtggt cagcaacagg agttgtggtg tgaaggtggt gaagtcgcct
    21781 ttatcaaaaa gatgattgaa gagagcaaag gcttcgcgaa gcaggtgatg tggtttacct
    21841 cgctggtatc tcgtggtgaa aacttaccgc cgttgtatcg tgctctgacc gacgtgggcg
    21901 cggtgaaggt ggtaaaaaaa gagatggccc aggggcaaaa gcagagtcgc tttattgcct
    21961 ggacctttat gaacgacgag cagcgccgcc gttttgtcaa tcgccagcgt taaagcgttg
    22021 gctcccccgg cggtaacggt tcagccgggg tcgcgcccgg agtaggcagc acctgataag
    22081 tctgcactgg cgcgcgaacg cccgccaggt cgaaatgttt tttcacctgg ctgtcgaggg
    22141 caaagcgtac cgtccactgt ttgagtggca gcgtggtgaa cgaaacacgc agtgtaaacg
    22201 cggtattgct taagccgaca atcccggcaa aattcggttc accaataatc agcccgcgaa
    22261 tttcttcgtt ttccattaat tccgctaccg catctttcag tgcctgattg gctttatcag
    22321 catcttcatg gcgatcaaca tcgtagtttg ccactaccga accaataccg cggacgaagt
    22381 tagcaaaggt ggttatcgaa gaccacggaa tgatgtgata cgccccggta tcctggcgca
    22441 cgcccacgga gcgaatcgac atccgttcca ctgtgccggt caacggcccg atagtcacca
    22501 aatctccagt gttcatgccg ttttcaaact gaataaacac cccggtaatg atatctttca
    22561 ccagcgtttg cgaaccaaac gagatagcca gacctaatgc cccggcacct gccagcaatg
    22621 gcgcgatatt gacgccgatt tccgacaaca caatcatgat ggtgatggta ctgataatca
    22681 ccgccagcgc gttacggaac agcgtcagca gggtacgcgt acgggcgctg ggtagcgggc
    22741 ggccatgaat atccgaagcc agccggtttt cgatcaaact ggcgagcacc gtccagccaa
    22801 ccgccgagaa aaaaagaata agtgcgatac ggatcaggat atctacggtt ttctgccccg
    22861 cgccgttttg cagccagttc cagaaatcga acaatcccca tgcgctcaac agcaacatta
    22921 ccgccacgca gacggtcaga atacgcgccg ttttcagcgc cgccgaaagc cagccattca
    22981 gccgtttttg caactctgga tagttacgct gagtatgtgg cgagagagtg atggtttttg
    23041 ccagccagcg ggaaaacata ccggaaacaa acgctgcaat accaataatc gccaggctgc
    23101 gcaccgttgc acccatcata aatttcaggc tgttgcccgg atcgaacaac gaaaagaaaa
    23161 acagcacgat aaaataggcg cttgccagcc agtgccacac cagcgcaaag gcgcggataa
    23221 acaggctgaa aaaggccagc gaatgctccg caaagttgag caaatgctgg gtaatctctt
    23281 ttttattacg aaagatcagg tacaacgccc agacggtcat gcacagcata atgatgacgt
    23341 tcgccagcgc acctatctgt acattcacct gattagagat aatcggcacg gccacaatca
    23401 ggccataacc tatcaaactg cttaaccagc tcaggcgacg actccagtaa cgggcgctct
    23461 cgtcctgaat cgtgaacggg cgcagctccg ccacgtttgg gcaaaaaatc aggcgtagta
    23521 cggctttgaa aaattcaatc agggcaaagg cgttgagaaa caaactttgt tggaaagcga
    23581 tggtgcgact gcctgcattc aggttgtcgc ttaatacctg gccgacaaat aatgtcagtg
    23641 ccagtaacag taggtcgata ataaacgccc cgataatcat cgccggaagc tgcaaccagt
    23701 tactgcgctc acgatttttt tgccgcgccc acaggcccat tttgcgatac agcggcagtg
    23761 cgcacaggcg aatcagccag taaaaaccaa acactaatac cgctaacatt gaaaaatggg
    23821 tcagcgcatt gctgaatgtt tgtggattaa acggcttatg cggggagccg gtaatattgc
    23881 gataaagttg cccgaagcgg gcggaaaggg cttcaccata atggcggctg acttcggtga
    23941 ccttttgcag cacggtttgc tcttcaacca gcgtcggcgg cacgatcttc gggaccggtt
    24001 ccgcaggggg cgtcgcggcg acagtacgca actggtcgat caattcttta cgcgaggtgt
    24061 cgttatccag cacatccgcc agtgcgccat aggccgcttt tttttgttcg atatccggtt
    24121 ccggggccgg ttcagtcgtt gagtccgttg tcgttgtggt tgtaacgccg ggtatggata
    24181 ccgcgtgggc aggtgcgccc agcaggcaga agaggatgaa caggatccac cgcatgactc
    24241 ctccagtgag aaaacagagc aaaaagataa gtatagatgc tgggggaggg gcgattttaa
    24301 atgagaggaa tctggtgtgt ctccctttcg ggtgaaaggg aggcagagaa gttaagagac
    24361 gtgctgcaaa aattcctgca agcgctggct cggcgggttc ttgatcaata cctgcggatc
    24421 gccatcttcc gcaatccggc ctttgtcgat aaagatcagc cgcgaagcta ctttctcggc
    24481 aaaaccgatt tcgtgggtca cgatcaccat cgtcatccct tcttcagcca gatcctgcat
    24541 aaccttcagc acttcatggc gcagttccgg gtcaagagcg gaagtcggtt catcaaacag
    24601 catcattttc ggcttcaccg ccagcgcgcg ggcaatcgcc acacgctgct gttgaccacc
    24661 agaaagttcg gaagggtagt gatgtgcacg ttctgccaga ccgactttcg ccagcagctc
    24721 acgcgccagt ttttccgcct cttctttgtt cgcgccacgc acgcgtagcg gaccaaacat
    24781 gacgttttcc agcgccgtca gatgcgggaa gaggtaaaac tgctggaaca ccatacctgc
    24841 ttcctggcga atcaggcgct cgtcaacttt cggatcgtta accttcaggc catcgacaat
    24901 cagatcgccg gaggtgattt cttccagttt gttgatgcag cgcagcaggg tcgatttacc
    24961 ggaaccggac ggcccgataa tcaccacgac ttcgccctgg gcaatgttca aatcgatatt
    25021 gtgcagcacc tgggttgggc caaagtgctt ggagacgttt ttaaattcaa tcacaggatt
    25081 ttcatccttc tttccagacg acgcagaata aagctcagca ctagggtaat aatcagatag
    25141 aacaccgcca cggcgctcca gatctcaagg gcgcggaagt taccggcaat aatttcttgc
    25201 ccctgacggg tcagttccgc cacgccgatc acaataaaca gcgaggtgtc tttaatgctg
    25261 atgatccact ggttacccag cggcggcagc atacgacgca gtgccagcgg taaaatgacg
    25321 tagcgaatgg tttcccaacg tgaaagaccg agcgccagtc ctgcttcacg aaaacctttg
    25381 tggatagaca gcaccgcacc acgcgtgatt tccgcaatgt acgcgccgga gttgatcatg
    25441 atggtgacca ccgccgcagt aaatgggtcg atgcgtaagt cgttaaacgc catcggcagg
    25501 gcgaaataga taaacatcac ctggacgacg ataggtgtgc cgcggatcac ttcaataaag
    25561 accagcgcga cgtggttggc tatccaacca ccgaaggtgc gtgcaaaacc tgccagcaat
    25621 ccgattacca gaccgccagc cagaccgagg accgaaatcc acagggtcat tttggcacct
    25681 tcaatcagaa gcggaatggc aggccagatg gcactccagt caaactgcat atgttgttcc
    25741 tgttaccgtg gtgtaaataa tcaaattcag ggtaattaat ggccccggag cgggtttgcg
    25801 cccctcaccc taatcctctc cccagagggg agagggaact gccagtgcgc tttacaggtg
    25861 tagcgttatt atttcggttc agtaccgaac cattttttgt agatttcgtt gtaagttccg
    25921 ttctcgcgca gggttttcaa cgcgccgttg actttgtcac gcagctcgtc gctacctttc
    25981 gggaacgcaa taccgtattg ctgcgcttcc agagagtcac ctaccgcttt gaactgaccg
    26041 ttaccggcgg ttttgatgaa gtacagaatg tttggcgtat cgtgcagaac ggcgtctgcg
    26101 cggttggtgc ccagttccat ataggcgtta tcgatgttcg ggaactgacg cagatcttta
    26161 gttttgatgt ttgctttcgc gtaatcaacg gagccagtac cgctcttcac agcaaccact
    26221 ttcccgtcga gatctttcac gcttttcaca tcgttattgt tagctttcac catcactaac
    26281 aggccgcttt tgtagtagcc gtcagagaaa tcgatcgctt ttttacgctc gtcggtgatg
    26341 gtaatgcccg ccagcgccag atcgacgttt ttggtttgca gtgccggaat gatcccactg
    26401 aaatccatcg gcttcagttc gtaatccagc ttcagctctt tagcgatggc agcccacaga
    26461 tcaacgtcaa agcccacata tttatcgccc tgtttaaatt caaacggaac gaaggcggta
    26521 tccgtcgcga caactaattt tttatccgcg gcatgagaag aaaccgcaaa agccagggtc
    26581 agtgcagcca gtgaaacttt taatacagac ttcatagcat ttcctttttt ctatccacgg
    26641 gacgatcccc tgcgtgacat tcacatatat gaaaaaatcg tgccagtttt gcaactcctt
    26701 gatttttcga gatgcggcaa aaacgtgatg cacgatttat agggcaatac cctgaagatg
    26761 caccattctg gggcaccaat ctggtgcgct aaaattgtgc actcaacaca gtgattattt
    26821 agcgtaaaac ctgatgaaaa aacaatcttt atgtaacgat tgtgtgatga tgtggataca
    26881 aaaaatttac ttaatcagct ggagatagca gatggatgca ctaaataagt gcgttgaggt
    26941 gggctgcaaa acaaaacggc ctcctgtcag gaagccgctt ttatcgggta ctaaagttct
    27001 gcaccatcag cgatggattt attcgatgtt agactcgata aaccacagga atttatccag
    27061 gtcgcgagac gcggcggtca ggatatctgc ggtgtcgtca tctttcgctt cgccaatcgc
    27121 tttgcgtacg tcattagcga cgattgcgta acggtcagcc agttctttca ggtgatcctg
    27181 aacgttgtgg atgtccagcg ggtaactttt cagcggggtt ttgctgttga taacttgagt
    27241 ggtccccaga gctacaccgc ccagctgcac tgcacgttct gccatggtat ccagatgatc
    27301 gatcagtgcg gtgcgaaagc catccagcat ttcatgtacg gcaatgaagt tagcgccgcg
    27361 catgttccag tgcgcttgtt tggtaatcaa agaaagatca ataaactgga taacctggcg
    27421 attcagcaac tctactgttg cttttttctc gctgtcggag acatcgttgc gggtataaag
    27481 cagattggtc gcttttgatt taactaattt agcggtactc ataatttcat atcctcttga
    27541 tgttatgtcc cagtaattaa cgagactaag tatagcaccg gctatgtgtt ccgctattct
    27601 ggctgttcct atcacactaa tagtggtaac aagcgtgaaa aacaaaacta ataaagattc
    27661 aatgagttag atatattgat aagaacaatt cttatttatc tgctcgccag gaattttcgg
    27721 aataaatatg cttttgcata attcgatata taaagacggt gtagaggaaa agtagcgaga
    27781 aattctgcat ggttatgcat aaccatgcag aaatgtaaat ttaattaatg tctaattctt
    27841 ttattttgct ctctttgcgt actgtcagcg tagaccccat tgaagcggcg atgatagcgc
    27901 cgagcgccag cagctgtatg ggtgtcagtg tttctccgag gaaaatcatc ccggaaacgg
    27961 cagccagcgc cggttccatg ctcatcagcg taccaaatgt ccgtgttggc aaacgggtga
    28021 gggcaatcat ttccagcgaa taaggcagag cggtcgagag aatagcgaca gccagaccca
    28081 atggaataac cgaccagtgc cagagtgctt caccagcctg aagcgctcca attggcacga
    28141 aaattaacgc tgcaatcaac gaaccaattg ccaccgtcgc agggccatgt tccgctcctg
    28201 cgcgttgccc acttaaaatg taaatagccc aacaagcccc ggcccccagt gccagcgcac
    28261 agccggttaa atcgacatgg gaaacgtctt gccccagcgg tagcaggaac cacagaccaa
    28321 gaaccgccag cacaacccag acgaaatcta ccgggcgacg agaagagaac agcgccaccg
    28381 ccagtggtcc ggtgaactcc agcgccaccg caatacccag cggtactgtc tgaatagaaa
    28441 gataaaaaag ataattcatc ccacccagcg aaacgccgta aaacaacagc ggtaaccgtt
    28501 gctctttggc aaagcgcagt cgccatggct taaagaacgc gatgaggatc agcgttccta
    28561 atgccagacg cagcgcagtg atacccggtg cgcccaccag aggaaaaagt gacttagcta
    28621 acgaggctcc accctgaata gacgccatgg caacgagcaa tatgattatt ggtaaccaga
    28681 ccggcatttt acgtaatgaa cctggcatcc tttctcccac aaatatctag acttaagtaa
    28741 agcgtggagt gtactggata tacccaatgc tggttgagca tttgttgaaa aaattttccc
    28801 ccgttttgac taaaatgcgc caggattgat ggaatcatta gtctggtgat taggaataat
    28861 ctggatgaat gacagggaaa acatgcgtaa tacttacgca gttctctgaa aaagtgattt
    28921 aaatttagat ggatagcggt gtatggaaac gttctgttac atgaaatggc ccgttagaca
    28981 tcacaaatcg cgaagagttt cccattaatt tttgatatat ttaaaactta ggacttattt
    29041 gaatcacatt tgaggtggtt atgaaaaaaa ttgcatgtct ttcagcactg gccgcagttc
    29101 tggctttcac cgcaggtact tccgtagctg cgacttctac cgtaactggc ggttacgcac
    29161 agagcgacgc tcagggccaa atgaacaaaa tgggcggttt caacctgaaa taccgctatg
    29221 aagaagacaa cagcccgctg ggtgtgatcg gttctttcac ttacaccgag aaaagccgta
    29281 ctgcaagctc tggtgactac aacaaaaacc agtactacgg catcactgct ggtccggctt
    29341 accgcattaa cgactgggca agcatctacg gtgtagtggg ggtgggttat ggtaaattcc
    29401 agaccactga atacccgacc tacaaacacg acaccagcga ctacggtttc tcctacggtg
    29461 ctggtctgca gttcaacccg atggaaaacg ttgctctgga cttctcttac gagcagagcc
    29521 gtattcgtag cgttgacgta ggcacctgga ttgccggtgt tggttaccgc ttctaatcac
    29581 tttggtgata taaaaaatcc gcctctcggg gcggattttt tgttttaagg tttcgggtcg
    29641 aaaatatcgg ttccgagatg gttgtaatcc accttctgta actggaagtt ggtgatgtat
    29701 atcggcccgg ctttcttctc agatataaac gggtatttga tatttatttc cttcgcttta
    29761 attcccgtcc actgcgagaa aaagcctaag aagtcattgg ctgagcggcg ggctttaatc
    29821 acccgatgcg ctttatcgtc gctggaaatg accataaaag gcacctggaa attttgctga
    29881 tatttatcat catgggcaag gtattgcacg tctttgccgc gctctttaaa ggccagaccg
    29941 tggtcagaaa agtaaaccag agagaagctg ctgccgctgt tgcgtaactg atcgtacagc
    30001 ttgcgcagta aatcgtccgt ttgcgtcatg gtatagagat agcacgacgt ttctttcgat
    30061 tgcacaaagg tttcgtattt gccttgtgtc ctgtcgcagg cctgcggatg cgagcccatc
    30121 agatgcagaa caatcagctg cggttgcgag tgctcttgcg ccagcacttg agcggtcata
    30181 tccagtaacg cttcgtcttt cgtgttttta tctgcttcaa aattaccttc tttcaggaag
    30241 tacacttcat ctgcacgttt ggcgatgctg gcgatagcag tatcgtattc gccgatttgg
    30301 ccctggttgg aaaaccacca ggtctggaag cccgcgcgat ttgccagggt gacaaagtta
    30361 tcctgaaact gtggtttgcc atcgactacg cgattgagcg ttaagccaag cgatttctgc
    30421 gtggagccac tggcggcaat gtagtcagca aatatcaaac cgttaacgct gctggcaaac
    30481 ggggtattgt cccagtgacc gccaaaggca ccgagggcat cgcgacgcgc gctttcaccg
    30541 atcaccacca cataggtctg atacttcggc ttaacggcag tgaccgtcca ggtatctttc
    30601 attccggaaa gttttgccat ccgttcttgc tcttcaatca cctcgttatt attgacgatg
    30661 acatccttag caaaacgaaa taccggatag ccagtatctt tcagtttaaa tacgccaccc
    30721 caggccaggt tttgcacggg agcaacaaaa aatgtcgcca cactgaatac cagacacagg
    30781 ctgtcgaagg tattccagcg cgctttttca ttctcttttt ttcgcctgat tgctattacg
    30841 ccgagcgcaa aaataaataa gccgaccagg tagctgtacc acggaaaaat cgtcaggatt
    30901 tcggtcgact cttccatatt ggtggagtgc aatgccagca atgtattgaa attaggcgcg
    30961 ccgtaggcct gagcaaaagg gaaataacaa gccgccacca gcgaactgac accgaccaga
    31021 actttttgta cgcgaggcaa tgttcgccat agcaaaagca aaatagccgt aaacgcagcg
    31081 gtgtagagca aactgaaggg gtaacctaag ccgaggttaa ttaatagcga ctgtaaaaag
    31141 tagaacgcag tccacgggct aaatacccgg ctacgggtaa caagcgattc tttgagggtt
    31201 aaattcatat gccactatcg taacaaacgc catgtgctta ccctggcgtc aagggtcaat
    31261 acctgcaaga gtgcgtgaag agtggaaagg ggtccgcatc cgcgagccgc aatatacgca
    31321 gggctgcaag aagatagagc gagccgccgt tcaggtcaac tactgacgaa caaatctttt
    31381 gcgaagggga ttgcaaatcc gacaaaaagt ggggtttttc agaccaaaac gcgttatgcc
    31441 tgcatgaaag caggagtgaa gcggcgtacc gcgacgccgc tatttatcgg aaggtttatc
    31501 ttgctgcggt ttgttgttga ccatatcgca caacatagag agcagcatta accgtacttt
    31561 aaagggagaa tgactaaaca cgcgcataca cctcttgaac tcattcataa gacctcctga
    31621 cttgctaatc ccgtcgatcc ttgagggatg attgcattac atacagatat agcacaggct
    31681 atattatata gctattgcta aaacgttaat tttttgtacc ttcgcaactc tggtttacaa
    31741 tgtgcgcacg aaatgagaac gctatgtatg cgtcaccata atgaggaagc acaatgagtc
    31801 gtcgcgcagg tacgccaaca gcaaaaaaag tgacgcagtt agtgaacgtg gaagagcacg
    31861 ttgaagggtt ccgccaggtc agagaggcgc atcggcgcga gcttattgat gattacgttg
    31921 agctgatttc tgacttgatc agggaagtgg gggaagctcg tcaggtggac atggctgctc
    31981 gtctgggagt ttcgcaaccg acggtggcta aaatgcttaa gcggctggca accatggggc
    32041 tgattgaaat gatcccctgg cgtggcgtgt ttttaacggc agaaggagag aagctggcgc
    32101 aggaaagccg cgagcgacat cagatagtcg aaaatttctt gttggtgttg ggcgtcagtc
    32161 cggaaatcgc ccgtcgcgac gcggaaggca tggagcacca tgttagtgaa gagacgctcg
    32221 acgcttttcg tttgtttacc cagaaacacg gtgccaaatg agcctgcctt ttttacgcac
    32281 gctgcaaggc gatcgttttt ttcagttatt aattcttgtt ggtatcggat taagcttttt
    32341 cgtgcccttt gcaccgaaat cctggcctgc tgctatcgac tggcacacca tcatcacctt
    32401 aagcggcctg atgctgctga ccaaaggtgt ggagttaagc ggttattttg atgtgctggg
    32461 gcgcaaaatg gtgcgccgct ttgctacgga gcgtcggctg gcgatgttta tggtgctggc
    32521 ggcggcgctg ctttctacct ttctgaccaa cgatgtcgcg ctgtttattg ttgttccgct
    32581 gactatcacg ctaaaaagac tgtgtgagat cccggttaat cggctgatta tttttgaggc
    32641 gctggcagtc aacgctggtt cgctactgac gccaattggc aacccgcaaa atattcttat
    32701 ctggggacgt tctggtcttt cgtttgccgg atttattgcc caaatggcac cgctggctgg
    32761 cgcaatgatg ctgacgctcc tgctcctgtg ctggtgttgt ttccctggaa aggcgctgca
    32821 ataccatacg ggggtgcaaa caccggagtg gaaaccgcgg ctggtgtgga gttgtctggg
    32881 gctgtatatc gtctttctga cggcgctgga gttcaaacaa gagctgtggg gactggtgat
    32941 tgtggcggca ggctttgcgc tgctggcacg tcgcgtggtg ctcagtgtgg actggacgct
    33001 gctgctggtg tttatggcga tgtttatcga cgtccattta ctgacccagc ttccagcgtt
    33061 gcaaggcgtg ttgggtaacg tgagtcatct atctgaaccc gggttatggt taacggcaat
    33121 cggtttatcg caggtgatca gtaacgtgcc gagtaccata ttgttgctga actatgtgcc
    33181 gccgtcttta ttactggtat gggcggtaaa cgtaggtggc tttgggttat tacccggatc
    33241 gctggcaaat ttgattgcgc tacgtatggc gaacgatcgc cgcatctggt ggcgtttcca
    33301 tctctattca ataccgatgc tgttgtgggc ggcgttggtg ggatatgttt tgttagttat
    33361 actcccggcc aactagggtc tggcaaataa aaaaggcgga ttatgagtcc gccttttgct
    33421 ttaagttttg aagattaatt cagacgaacc ggcatcccgg agcggttttt aatcgcttca
    33481 tcaagaacaa cctggtcaac atctggctga ccggtcactg tttgcacgct cttcgtcagg
    33541 gtaattggca caatttcctg accttcaaac tgggcttcgg tggtagacag cgggttatgg
    33601 acttcaatat aacggctgcc gtctggctcg gtggtcgctt ttaccggctc atcaataaac
    33661 tgtacgcggg taccgaccgg tactttctcg aacaggaatt tgatgtcttc gttacgcaga
    33721 cgcacacaac catgacttac acgcaggccg ataccgaagt tggcgttggt gccatggata
    33781 gcgtacaggc gaccgatgta gagtgcatac agccccatcg ggttatccgg acctgccgga
    33841 acgacagccg gaagcggttc gcccgcagcg cggtactctg cgtgcatttt ggcggtcggc
    33901 gtccaggtcg ggcctgcttt cttacgctca actttggtgg tccagttgat aggcgtatct
    33961 ttgcctaact gaccaatgcc gatcggcagc acgataacgg tgttggtccc tttcggatag
    34021 taatacagac gcatctctgc actgttaatg acgatgcctt catgaacggt atccggcagg
    34081 atcagctgct gcggaatgtt cagtacagta ccgcctttcg gcaggaaggt atccacaccc
    34141 gggttcgctt ccatcatatt ggaaagcccc atctggtact ccgcggcaaa atactccagc
    34201 ggctgagtgt taccttcagg aatggtgatc acctgattct gaccaaccag gcgactcccg
    34261 tcggttggca gaggataagt taccgcagag gcggtactgc aaaagccgac aacagcgaag
    34321 gccgctgcga ataatgtttt caatttcata ttcatgttaa gcgagatttt gtgcctggca
    34381 ggccattggg ttgagaatat tagagtattg gaagcgcatt ataagttcat tccagctcac
    34441 agtgaaatca gatgtgtacg aaatcacatt ttttgccttt ggcttgagtg tagaccttaa
    34501 gcgaggagca ggatcttctt tcagacttat ggcataatgc gcggtttgtc atatctcttt
    34561 tcaggatacg cctgtgttag tttccagtaa cgtcaccatg cagttcggca gtaagccgtt
    34621 gtttgaaaac atttccgtca aatttggcgg cggcaaccgt tacggcctga ttggcgcgaa
    34681 cggtagtggt aaatccacct ttatgaagat cctcggcggc gacttagagc cgactttggg
    34741 taacgtttcc ctcgatccca acgagcgcat tggtaagctg cgtcaggatc agtttgcctt
    34801 tgaagagttc actgtgctgg atacggtgat catggggcat aaagagttgt gggaagtgaa
    34861 gcaggagcgt gaccgcatct atgctttgcc ggaaatgagt gaggaagacg gctataaagt
    34921 ggccgatctg gaagttaaat acggcgaaat ggacggttac tctgcggaag ctcgcgccgg
    34981 tgaattgttg cttggcgtgg gaattccagt ggaacagcac tacggcccga tgagtgaagt
    35041 tgctcctggc tggaagctgc gtgtgcttct ggcgcaggcg ctgtttgctg atccggatat
    35101 tctcctgctc gacgaaccga ccaacaacct cgacatcgac accattcgct ggctggaaca
    35161 ggtgctgaac gagcgtgaca gcaccatgat catcatctcg cacgaccgtc acttccttaa
    35221 catggtctgt acccacatgg cggatctgga ttacggcgag ctgcgcgttt atccgggtaa
    35281 ctacgatgag tacatgacgg cggcgaccca ggcgcgtgaa cgtctgctgg ccgataacgc
    35341 caagaagaaa gcgcagattg ctgagttgca atctttcgtt agccgcttta gcgccaacgc
    35401 ctcgaaatct cgccaggcaa cttcgcgcgc gcgccagatt gataaaatca aactggaaga
    35461 ggtgaaagcc tccagccgtc agaacccgtt catccgtttt gaacaggata agaaactgtt
    35521 ccgtaacgcg ctggaagtgg aaggtctgac caaagggttt gataacggtc cactgtttaa
    35581 aaatctcaac ctgctgctgg aggtcggtga aaaacttgcg gtactgggta ccaacggcgt
    35641 cggtaaatca acgctgctga aaacgctggt gggcgatctg caaccggaca gcggcaccgt
    35701 aaaatggtct gaaaacgcgc gcattggtta ctacgcccag gaccacgaat atgagtttga
    35761 aaatgatctg accgtgttcg aatggatgag tcagtggaag caggaaggcg atggcgagca
    35821 ggcggtacgc agcattctcg gtcgtttgct gttcagccag gacgacatca aaaagccagc
    35881 taaagtgctt tccggtgggg aaaaagggcg gatgctgttt ggtaagttaa tgatgcagaa
    35941 gccgaacatt ctgatcatgg acgaaccgac caaccacctg gatatggaat ccatcgagtc
    36001 actgaacatg gcgctggaac tgtatcaggg cacgctgatc tttgtttcac acgaccgtga
    36061 gttcgtaagc tccctggcga cccgcattct ggaaatcacc ccggaacgcg tgatcgactt
    36121 tagcggtaat tacgaagatt acctgcgtag taaagggatc gagtaagtga ttatccgtca
    36181 ccccgatact gtcggggtga cggcggctga tgacaaatgc aaaatagcct gatgcgctac
    36241 gcttatcagg cctacgcagt acctgtaata tattgaatct gcaggacttt gtaggccaga
    36301 taaggcgtta acgccgcatc aggcattaag aaagcgcact tgtcagcaat ctgagtaacg
    36361 tcgaatcgat tttcttaatc ccacatcaat tcacttccat taactctcaa acatccatgc
    36421 ccaacgctcg cttaccgtga atgttgaggt cggcaagcgt aaagcgtcct tcccagtttg
    36481 tttcatagtc ctcgcgcggg aagtcgcccg gcgatgcgcc tttttccagt gcggccttca
    36541 ccttgtgcgc gtaggcaaga tttttctcgc acatcggtgc cgcaggaatg tacatcacgt
    36601 tgccccaacc ctgttgattt tcaacggggg caacggaatg gatgacgtcg cagtgccacc
    36661 agacggagtc tccggcctcg agttttggaa tgctggttaa cgcctcaatt aacagtggat
    36721 gccattgctc tgataccggc aacactcttc cgggcgctac gccgcacagt tcatcctccg
    36781 gcacatcatc aagcagcgga cgtaacagta cgtacgccat ggcttcagga atgggcacaa
    36841 cgtgcagcaa cccctgacca ggcagcatat cagagagcgc tgtccagccc tggaatgtcc
    36901 gaaacacgga acatttggtg gtgttgtcca ccgtgtactc ttcaacttcc gtacgatgtg
    36961 ccgcatgcca gggatcatat tgcgccagat tgccattaaa gacgttggcg aaaacgcgct
    37021 gatacgctgg aagcagccag cgttccagcg ccccggagtc ggtatgcgct ccaagacctt
    37081 tggaggtcgt tccgggcgga cggcggcgga tacggtcagg gtagatgacg ctcacatccg
    37141 ggttaaacca ttgctttcca tcactttcaa atgtccacag acgattgaga aacgactgcg
    37201 cattcgccat ttcttcactc tggcgggcct gcatttgcgc ctgcgaccag tagatggggt
    37261 aaatctcggg acgtgaagcg ctgagcgtcc cgaagaaatt atcgccgggg cctttgtaga
    37321 cctcgtcaaa gcggttgcgg tccagatagt ccagcatcga ctgatcccag cctagcgctt
    37381 gttcgcgggg gaaatggcct tttatcaccg cacaaccgcg acgtttaatc tgttcgcgct
    37441 gctctgcagt aacatgacct gctttgatat cggcataaga cagcaccggc cagacggcat
    37501 cgccctgtgc tttgagtgcg ttgatttcag ccactcgcgt ggcaatgtca tcgcttagct
    37561 gattaaagat ctgctggacg tcgccaagct gcgcccgcag cgcgtgcttc atctgacgga
    37621 tagctgcttt gtgatcggca ggcaatgtgt cgctggtaaa agtagaagcc ataaccacct
    37681 ctcattaact ttcattcgaa accattctga tttgcatgtg acaatataag ttataaataa
    37741 gttaatgcaa gtttaatgat ttgatgtgat gtacaggatg taaaaagaag ggaaaaccgg
    37801 agcgcacggc tccggtagag aggtcagctg ttaaaagggg atgtgttatc cagcaccgcc
    37861 tgaatcacat tcagcgcgcc ttcatgatta ttatcatcgg tagcgtaacg ggcgatttgt
    37921 ttaatgtttt ccgcagcatt gcccatcgca aaggaataac gcgccatttt cagcatctcc
    37981 gcatcgttac cgctgtcgcc aatcgctacc acattttgcg gtgacagatc ccagcgtttc
    38041 agtaaccgcg aaataccgtt tgctttatgt agaccgggaa taatcaggtc gataaagcca
    38101 aaaccactgg tgacgggttt cataatgcca tcgagcgcta tgtgcagttt gtcgatcact
    38161 aacgggattt gttcatctgg caggttgagc gaaaacttga acagtacgta gtcaatctcc
    38221 tgataatctt ttacagcttt caggcgatgg tagtgttttg ccatcagtgc gacaaatgct
    38281 tcgggggcat tttcgctgac atatgcactt tgcagaccgc aggcgacaaa attgagttgc
    38341 ttatctttta gcaactcgcc aataacaatc cgcgattcat gtcgggtcag ttcgccgtgg
    38401 aacagttgct tgccatgttc gtaaaccagt gcgccgtttt ccgcgacaaa agagatctca
    38461 tcctttagct caggaaagaa cgaaataagc tggtaatact gattaccgct ggcgacaacg
    38521 aactcaatgc cgcgcttttt cagttcctga tattgcgcca taaaacgtgg ttggttgtac
    38581 gttttggcgt cgttaagaaa agtaccgtcc atgtctgtga cgataacttt tacgctcata
    38641 ggtgtgctcc tggctcgaaa atgaaaccgt aacagtgtaa taacaatgtg acgcagagca
    38701 caaattatat ttcgaatgaa agtaaggatg aaattgatga tgtgaatgat ttagcccggc
    38761 gacgccgcag ccgggccgaa gagattacag catatgttca gtacgagcga tgatatcgtc
    38821 ctgagcgtct ggagagagcg cggtgaagaa ggcggaataa cccgcgacac gcactaccag
    38881 atcgcggtac tgatccggat gttttttcgc ctccagcagc gtttcgcggg aaacgatgtt
    38941 gtactgaata tgccagcctt tatgcacctc gaagaaggtg cgcagcagga tcatcagctt
    39001 ctgcttgtca gattcgttct ccagcgttgc cggattcagt ttctggttga gcaacacgcc
    39061 gccgagaatc gctgccgtag gcagtttacc caccgagcca atgaccgcag tagggccgag
    39121 atggtcagta ccagaggccg gactagcgcc ttctgccagc ggggtgtggg ctttacgccc
    39181 gtccggcgtt gccatagttt gcgcgccaaa cggtacgtta gcggagatag atgacgtacc
    39241 cgcgtaatag ttgccgccaa ccggaccacg accgtagcgc ggattatggt actgtttcag
    39301 ttcgtcgata taggtctgat aagcgcgagc cagcagcgta tcgacagtat catcgtcgtt
    39361 gccgtacttc ggcgcaccgt taatcagccg ctgacgcagt tgctcatgag tcaggccatc
    39421 gaagtcatcc gccagtgcgg cagccagctg ttgctgacca atcgcgccct gttcaaatac
    39481 cagtttcttc actgccgcca ggctgttgcc gaggttggca atgccaacct gcaggccaga
    39541 aacccagtca tatttcgcgc cgccttgctt gatacttttc gctcgctcaa tacagtcatc
    39601 caccagcgcc gagcagagaa tatcgtgtac gttctcttcc agcatggtgt cgacgacata
    39661 ttcgatttcg attgatttac gcgtgtaata acggatttgc gtatcccacg cgtccattac
    39721 ttcatcgaaa ttgttaaagt tgcctgccga caacgctttt tcttgtggca ggaacacttt
    39781 gccgctggtg gcatcacgcc cgccttccag cgccgccagc atcacgcggg cgaagttgat
    39841 aaagctcatg ccggtacagc gatagcccca tttgccaccg acggcggttt cgatacaacc
    39901 aatcgctgcg tagtcgtaag cgtcctgcgg ttcaataccg agtttaataa attccgggat
    39961 cacgatttcg tcgttgttga acgccggcat cccgaagccg caacggatca cctgtacgca
    40021 ggcgtcgagg aaatcattac tcattcctgc atggtaacgc acgctgaggt taggctgagt
    40081 ggaacgcagg cgaccgcagg attcgaggat cgcgtaagag aggggattca ctgcatccat
    40141 tggttgacca tcaaccagat tttgtccgcc aatggtaacg ttctgataca gcggacttcc
    40201 cgcagaggct tttgagtgtg agccggagcg gatcttgttc acttccagca gtttcagcca
    40261 gcagctatgc agcatctcga tggcgtgttc gcgatccagc gtctggttga gttcaacgtc
    40321 gcggcgatag tacgggtaga gatactggtc catacgacca aacgataccg agtgaccgtt
    40381 agattcgatc tgcaaaatca attggatgaa gtaacacagt tgcagcgcct gccagaaagt
    40441 ctgcggcggc tggtgggcga taagatcgca gttttctgcc atcgtcagca gttcatcgcg
    40501 acggctttcg cgagtttcgg tagcagccat ttcacgcgcc agggcagcga aacgttcaat
    40561 gtgttcactg actgccacca gcacgatatc aatcgctttc aggaattgtt cgccatgtaa
    40621 atcttccagc accgtcagat tgatgcgcga gcgacgttcc gctactttct cacgcagacc
    40681 atcaagacct ttttccagca gcagcgggaa attcaccgcc aggtgcgcat cgccggaggt
    40741 catattgcct tccgctttaa tgattccggt cgccagcaga cctttttgct catcggtaaa
    40801 catgccgtag cagcgatcct gtacggtctg accgcgccac cacgggcaca cttcatgcag
    40861 aacgcgtttg ttctcttcgc tcaccgcaaa gccagcaccg ggacgatctg ccagatcatc
    40921 aatctctttt tcgatccacg agacagtata ttccgggaag atcggcgcgg cgcgaacttc
    40981 gcttgcctgg ttgccaatga tcaactcatc gtgtttgatc cagatggtgc gattcgccag
    41041 gtgatgcgcc agtgccagcg cgcgacgtac cgggatcggc ttatcgagat gttgttgata
    41101 catctcggta tagtgctgcg cgcgctcggt acagactggc ggtttcacaa tatgcaccag
    41161 cgcatttttg tgcgctttaa tgcggtcgct gagcgtgtcc agtttcagtg tggtcatggt
    41221 tgttatcctc gtaaggtcgc ggttaaccct ttctggcagg catactgctg ggcaaagtcg
    41281 agcagttctg gcgcatcaag cggtttttcc ggggcgtcat agggcagatt aagtaagtga
    41341 tatttgttga tgcccagcgt gtggtagggc agaaaatgaa tttcgccaac gtgcagctcg
    41401 tcggcggcaa aatcggtaat agcttttaca gaggtttcgt cggcattaaa gccctgaatc
    41461 agcggcacgc ggataatgat ttttttgccc gctgcggcga gttttttcag gttatccagc
    41521 actctggcgg cgttaccgtc ggtccactgt ttaaacggcg cgtcggcaac gtgttttaaa
    41581 tcggcaagaa acagatcgat atagggcaga gaaggggcga tatatttcca cggcacatgc
    41641 agacaggttt ctaccgcagt atgaatgcct gcctcgtggc tggcttgcag tagcgccatc
    41701 gccatttccg gctgcataaa gggttcacca cccgaaagcg ttaaaccgcc gccgctgcga
    41761 tcgtaaaacg gtttatcgcg cagaacggtc gtcatgatct cctcaacgct tttcacttca
    41821 ccacacacgg ttaatgcctg tgtcggacag cagtcggtta acgccgtcag atgctccggg
    41881 gttaactttt cccgatgaat aagcaaacca ttcagcgcgc gctcaatcac ttccggcgcg
    41941 gccttagcgc acagctcgca gccttccaga cacagtcgtg cgtcatacag cagatcctgc
    42001 gtgcgggcgc ggctttccgg gttctgacac cagcggcagc ccagcgaaca gcctttaaga
    42061 aatacgaccg tgcggatacc ggggccatca tgggtcgagt agcgctgaat attgaaaatc
    42121 atagttgcct ctctgtttcg ttcaaacatt aaaatacttt cgaatgaaag ttagattgat
    42181 gtgcgtcaac tgttcagaga gttttcccgt gatagtctac attcagacaa aaagtacatt
    42241 ttgaggatgg ttatggaact gtatctggat acttcagacg ttgttgcggt gaaggcgctg
    42301 tcacgtattt ttccgctggc gggtgtgacc actaacccaa gcattatcgc cgcgggtaaa
    42361 aaaccgctgg atgttgtgct tccgcaactt catgaagcga tgggcggtca ggggcgtctg
    42421 tttgcccagg taatggctac cactgctgaa gggatggtga atgacgcacg taaattgcgt
    42481 tctattattg cggatatcgt ggtgaaagtt ccggtgaccg ccgaggggct ggcagctatt
    42541 aagatgttaa aagcggaagg gattccaacg ctgggaaccg cggtatatgg cgcagcacaa
    42601 gggctgctgt cggcgctggc aggtgcggaa tatgttgcgc cttacgttaa tcgtattgat
    42661 gctcagggcg gtagcggcat tcagactgtg accgacttac accagttatt gaaaatgcat
    42721 gcgccgcagg cgaaagtgct ggcagcgagt ttcaaaaccc cgcgtcaggc gctggactgc
    42781 ttactggcag gatgtgaatc aattactctg ccactggatg tggcacaaca gatgattagc
    42841 tatccggcgg tggaagccgc tgtggcgaag tttgagcagg actggcaggg agcgtttggc
    42901 agaacgtcga tttaaccgga cgttctgcat cctcataaat tgctgatgac gtggcggagt
    42961 gccgcgtctt atcaggcctg gaggtggcaa ttactgcccg cacacctcac accccggatt
    43021 gcgcatcagt ttcatttcgc gaaactgaca ggtcatcgca tcgtacatga cgattttccc
    43081 gctggccggt ttgccataac ctgccagcag tttgatcgct tccattgcct gcaacgaacc
    43141 aattacgccg atcaacggtg ccatcacgcc tgcttctacg caggttaatg cgttctcgcc
    43201 aaacaaacgg ctaaggcagc gatagcacgg ttcgccgtcc tgataagtga agaccgtgat
    43261 ttgcccttcc atacgaattg ccgcgccgga aaccagcggc accttcgcgg caaaacagcc
    43321 tgcgttcagt tgattacgta ccgcaacgtt atccgtacag tcgagcacca gatcgtgttc
    43381 agcaatcaat gctgcaagtt ctgcgtcatc tagcagtgca ttgactggcg taatcgcgat
    43441 atgtgggttg atccgcgtca gggcgtcacg ggcggattcc accttcggtt gcccgaccgt
    43501 ggcatcactg tgcagtgtct ggcgttgcag attcgagagc gaaaccgtgt cgaagtcgag
    43561 cagcgtcagg ttgccgacac cggcgcttgc cagatactgc gaagctgcgc agccgagtcc
    43621 accgaggccg actaccagca cacgagaatc ttttagcgcc tcctgaccgt caaaatcaaa
    43681 accgcgcaga atgatttgcc ggttgtagcg cagcatctcc tgatcgctga gttccgccat
    43741 tacaggcctc cgaacaacgc gttaaacggt tctacttcca cccattcgcc cacgtccaca
    43801 ttgccgcgat cgcgttccag cacgataaag cagttgccga gactaaagga gctaaatata
    43861 tgtgaaccct gatgtccagt ggtcgtcact tcaagttcac cgtcggcgtt gcgttgcagc
    43921 acgccgcgct ggaaatcaag acgtcctggc gtttttttca ggcgggacgc tgtgcgtacg
    43981 cgctggcgcg cgggcaggcc gctggcggtg ttgccgctta gttttgccag caaaggctgt
    44041 accagttgat agaaggtcag cgtcgctgaa accgggttgc ccggcaggcc gcagaaccag
    44101 ctattgctga gtttaccgaa cgcgaacggt ttacctggtt taatcgccag cttccagaag
    44161 gcgatctccc ccagctcttc aagaatcgtt ttggtgtaat ccgcttcccc cactgaaaca
    44221 ccgccggaac tgatcactac atccgcctgg ctgtcggctt caataaatgc ggcgcgcagg
    44281 gcatggggat cgtcgcggat aatccctaag ttgattacct cgcaacccag ctgttctaac
    44341 atcaggtgta cggcgagacg gttggtgtcg tagatttggc cgtcgcccag cggctgaccg
    44401 ggcaactgga gttcatcacc ggtagaaaaa agcgctacac gcactttacg aatcaccgga
    44461 acttcggcaa tccccagtga agcaattact ggcagctctg cggtagtcag gcgagttccc
    44521 gccgggaaaa caaccgcacc tgcagagata tcttcaccgc gacggcgaat attttgcccg
    44581 ctacgcactt cagcagtaaa acgcacgcca ttgtccgttt gttcagtctg ctcctgcatc
    44641 accaccgctt cgcagccttc cggcaccggc gcaccggtca taatacgaat gcaggtaccc
    44701 gcaggccatt caccatggta tggctgaccg gcaaaggatt taccggcaac gggcagcggt
    44761 tgcccggagg caatatcggc taaacgcacc gcgtagccgt ccatagcgga gttatcaaac
    44821 cccgggacat ccagtggcga aacgacatcg ctcgccagaa tacgaccaaa acactgaacc
    44881 agtggcagcg tttcctgggt ggtcagtggg gtaacgcgag aaagcatctc attaagcgcg
    44941 gtgtcgagcg acatcaatcc ggtggtaaat tccatgaaaa cactcctgcg gaggcaaaat
    45001 cgaatttgcc tattatgtca gaaaaacgcc atagactgta tgccacctcg ggcgtagcgt
    45061 tggctcctgc ctttacatgc catatccatc tttctatatt caaaaattga atgagtaatt
    45121 cataaaaatt ctgatattta tagcaaaagt ggcgagccac ccttaatgga cgaatactat
    45181 gggcaaagca gtcattgcaa ttcatggtgg cgcaggtgca attagccgcg cgcagatgag
    45241 tctgcaacag gaattacgct acatcgaggc gttgtctgcc attgttgaaa ccgggcagaa
    45301 aatgctggaa gcgggcgaaa gtgcgctgga tgtggtgacg gaagcggtgc gtctgctgga
    45361 agagtgtcca ctgtttaacg ccggaattgg cgctgtcttt acgcgtgatg aaacccatga
    45421 actggacgcc tgtgtgatgg atggtaacac cctgaaagcc ggtgcggtgg cgggcgttag
    45481 tcatctgcgt aatccggttc ttgccgcccg gctggtgatg gagcaaagcc cgcatgtgat
    45541 gatgattggc gaaggggcag aaaattttgc gtttgctcgt ggcatggagc gcgtctcgcc
    45601 ggagattttc tccacgcctt tgcgttatga acaactactg gcagcgcgca aggaaggggc
    45661 aaccgtcctc gaccatagcg gtgcgccact ggatgaaaaa cagaaaatgg gcaccgtggg
    45721 ggccgtggcg ttggatttag acggcaattt ggcggcagcc acgtccacag gcggaatgac
    45781 caataaatta cccggacgag ttggcgatag tcccttagtg ggtgccggat gctacgccaa
    45841 taacgccagt gtggcggttt cttgtaccgg cacgggcgaa gtcttcatcc gcgcgctggc
    45901 ggcatatgac atcgccgcgt taatggatta cggcggatta agtctcgcgg aagcctgcga
    45961 gcgggtagta atggaaaaac tccctgcgct tggcggtagc ggtggcttaa tcgctatcga
    46021 ccatgaaggg aatgtcgcgc taccgtttaa caccgaagga atgtatcgcg cctggtgcta
    46081 cgcaggcgat acgccaacca ccggtatcta ccgtgaaaaa ggggacaccg ttgccacaca
    46141 gtgatgaact tgatgccggt aatgtgctgg cggttgaaaa tctgaatatt gcctttatgc
    46201 aggaccagca gaaaatagct gcggtccgca atctctcttt tagtctgcaa cgcggtgaga
    46261 cgctggcaat tgttggcgaa tccggctccg gtaagtcagt gactgcgttg gcattgatgc
    46321 gcctgttgga acaggcgggc ggtttagtac agtgcgataa aatgctgttg cagcggcgca
    46381 gtcgcgaagt gattgaactt agcgagcaga gcgctgcaca aatgcgccat gtgcgcggtg
    46441 cggatatggc gatgatattt caggagccga tgacatcgct gaacccggta tttactgtag
    46501 gtgaacagat tgccgaatca attcgtctgc atcagaacgc cagtcgtgaa gaagcgatgg
    46561 tcgaggcgaa gcggatgctg gatcaggtac gcattccgga ggcacaaacc attctttcac
    46621 gttatccgca tcaactctct ggcgggatgc gccagcgagt gatgattgcg atggcgctgt
    46681 catgccgccc ggcggtgctg attgccgatg agccaaccac cgcgctggat gtcactattc
    46741 aggcgcagat cctgcaatta atcaaagtat tgcaaaaaga gatgtcgatg ggcgttatct
    46801 ttatcactca cgatatgggc gtggtggcag agattgccga tcgggtactg gtgatgtatc
    46861 agggcgaggc ggtggaaacg ggtaccgtcg aacagatttt tcatgcaccg caacatcctt
    46921 acacccgtgc gctgttagct gctgttccgc aacttggtgc aatgaaaggg ttagattatc
    46981 cccgacgttt cccgttgata tcgcttgaac atccagcgaa acaggccccc cccatcgagc
    47041 agaaaacggt ggtggatggc gaacctgttt tacgagtgcg taatcttgtc acccgtttcc
    47101 ctttgcgcag cggtttgttg aatcgcgtaa cgcgggaagt gcatgccgtt gagaaagtca
    47161 gttttgatct ctggcctggc gaaacgctat cgctggtggg cgagtctggc agcggtaaat
    47221 ccactaccgg gcgggcgttg ctgcgcctgg tcgaatcgca gggcggcgaa attatcttta
    47281 acggtcagag aatcgatacc ttgtcacccg gcaaacttca ggcattgcgc cgggatattc
    47341 agtttatttt tcaggaccct tacgcttcgc tggacccacg tcagaccatc ggtgattcga
    47401 ttatcgaacc gctgcgtgta cacggtttat tgccaggtaa agacgcggct gcacgcgttg
    47461 cgtggttgct ggagcgcgtg ggcctgttac ctgaacatgc ctggcgttac ccgcatgagt
    47521 tttccggcgg tcagcgccag cgcatctgca ttgctcgcgc gttggcattg aatccaaaag
    47581 tgatcattgc cgacgaagcc gtttcggcgc tggatgtttc tattcgcggg cagattatca
    47641 acttgttgct cgatctccag cgtgatttcg gcattgcgta tctgtttatc tcccacgata
    47701 tggccgtggt agagcggatt agtcatcgtg tggcggtgat gtatctcggg caaattgttg
    47761 aaattggtcc acggcgcgcg gtcttcgaaa acccgcagca tccttatacg cgtaaattac
    47821 tggcggcagt tccggtcgct gaaccgtccc gacaacgacc gcagcgtgta ctgctgtcgg
    47881 acgatcttcc cagcaatatt catctgcgtg gcgaagaggt ggcagccgtc tcgttgcaat
    47941 gcgtcgggcc ggggcattac gtcgcacaac cacaatcaga atacgcattc atgcgtagat
    48001 aacattcagg cggagaataa aatggcaaga gctgtacacc gtagtgggtt agtggcgctg
    48061 ggcattgcga cagcgttgat ggcatcttgt gcattcgctg ccaaagatgt ggtggtggcg
    48121 gtaggatcga atttcaccac gctcgatccg tatgacgcaa atgacacgtt atctcaggcc
    48181 gtagcgaaat cgttttacca ggggctgttc ggtctggata aagagatgaa actgaaaaac
    48241 gtgctggcgg agagttatac cgtttccgat gacggcatta cttacaccgt gaaattgcgg
    48301 gaaggcatta aattccagga tggcaccgat ttcaacgccg cggcggtgaa agcgaatctg
    48361 gaccgggcca gcgatccggc gaatcatctt aaacgctata acctgtataa gaatattgct
    48421 aaaacggaag cgatcgatcc gacaacggta aagattaccc tcaaacagcc gttctcagcg
    48481 tttattaata ttcttgccca tccggcgacc gcgatgattt caccggcagc gctggaaaaa
    48541 tatggcaagg agattggttt tcatccggtg ggaaccggac cgtatgaact ggatacctgg
    48601 aatcagaccg attttgtgaa ggtgaaaaaa ttcgcgggtt actggcagcc aggattgccc
    48661 aaactggaca gcataacctg gcgtccggtg gcggataaca acacccgcgc ggcaatgctg
    48721 caaaccggtg aagcgcagtt tgctttcccc attccttacg agcaggccac actgctggag
    48781 aaaaacaaaa atatcgagtt gatggccagt ccgtcaatta tgcagcgtta tatcagtatg
    48841 aacgtgacgc aaaagccatt cgataacccg aaggtccgtg aggcgctgaa ttacgccatt
    48901 aaccgcccgg cgctggtgaa agtagccttc gcgggctatg caacgccagc tactggtgtg
    48961 gtagcgccaa gtatcgccta cgcgcaaagc tataaaccgt ggccttacga tccagtgaaa
    49021 gcgcgcgaat tactgaaaga ggcgggatat cccaacggtt tcagtaccac gctgtggtcg
    49081 tcacataacc acagcaccgc gcagaaagtg ctgcaattta cccagcagca gttagcgcag
    49141 gtcgggatta aagcccaggt gaccgcgatg gatgccggac agcgggcggc agaagttgaa
    49201 ggtaaagggc aaaaagagag cggcgtgcgg atgttctaca ctggctggtc ggcttcaacc
    49261 ggcgaagcgg actgggcgct atcgccactg ttcgcctcgc aaaactggcc accgacgctg
    49321 tttaataccg cattttacag caataaacag gtggacgact tcctggctca ggcactgaaa
    49381 actaatgatc cggcggaaaa gacccgcttg tataaggcgg cgcagtatat catctggcaa
    49441 gagtcgccgt ggatcccgct ggtggtagaa aaactggtgt cggcacacag taaaaacctg
    49501 accggttttt ggatcatgcc agacaccggc ttcagctttg aagacgcgga tttgcaataa
    49561 gcaacgcagg gagtggaatg cttaattacg ttatcaaacg cttactgggg ttgattccga
    49621 cgctgtttat cgtctcggtg ctggtgtttt tatttgtgca tatgctgccc ggcgatccgg
    49681 cgcgattgat tgccgggccc gaagctgatg cgcaggttat agaactggtg cgtcagcagc
    49741 tggggttgga tcagccgctg tatcaccagt tctggcacta tatcagcaat gctgtgcagg
    49801 gggattttgg cctgtcgatg gtgtcgcgtc gtccggttgc cgatgagatt gccagccgct
    49861 ttatgccaac gctgtggctg accataacca gtatggtctg ggcggtgata tttggtatgg
    49921 cggcgggaat tatcgccgcc gtctggcgta atcgttggcc ggatcgattg agtatgacca
    49981 ttgcggtgtc ggggatctcg tttccggcat ttgcgctggg gatgctttta attcaggtat
    50041 tctccgttga actgggctgg ctgcctaccg tgggagcaga cagttggcag cactacattt
    50101 taccctccct gacgctcggc gcggcagtgg cagccgtgat ggcgcgcttt acccgcgcgt
    50161 cgtttgtcga tgttgtaagc gaagattata tgcgtaccgc gagggcgaaa ggggtgagcg
    50221 aaacctgggt tgtcctcaaa cacgggctac gtaacgcgat gatcccggta gtgaccatga
    50281 tgggcttaca gtttggcttt ttgctcggtg gttctatcgt ggtggagaaa gttttcaact
    50341 ggcccggact tggacgctta ctcgttgact ccgtagaaat gcgcgattac ccggtgattc
    50401 aggcggaaat tctgcttttc tcgctagaat ttattcttat caacttagtg gtggatgtgc
    50461 tttacgccgc cattaacccg gctatcaggt acaagtaagg atgcgactat ttaactggcg
    50521 acgtcaggcg gtgttaaacg ccatgccact ggtcaaacct gaccaggtgc gtacaccgtg
    50581 gcatgaattc tggcgacgat ttcgccgtca gcatatggcg atgaccgccg cattattcgt
    50641 tattttattg attgtggtgg ccatttttgc acgctggatc gctccctatg acgccgaaaa
    50701 ttattttgat tatgacaatc tgaataacgg accttctttg cagcactggt ttggcgtcga
    50761 ttcactgggg cgcgacattt tcagccgtgt cctggttggt gcgcaaatct cgctggcggc
    50821 gggcgtgttt gccgtgttta tcggtgcggc gatcgggacg ttgctgggct tgttggctgg
    50881 atattatgaa ggctggtggg atcggctgat catgcgcatt tgcgatgtgc tgtttgcctt
    50941 cccgggtatt ttactggcga tcgctgttgt tgcggtgttg ggaagcggca ttgctaacgt
    51001 gattatcgca gtcgccattt tttccatccc cgcgtttgcc cgactggtgc gcggcaacac
    51061 gctggtgttg aaacagcaaa cctttattga gtcagcacgc agtattggtg ccagcgatat
    51121 gaccatttta ttgcgtcata tcttgcctgg gaccgtctcg tctatcgtgg tgtttttcac
    51181 catgcgcatt ggcacctcga ttatctctgc cgccagcctg tcatttctcg gcctcggtgc
    51241 gcagccgccg acaccagagt ggggagcaat gctcaatgag gcacgagcgg atatggttat
    51301 cgcgccgcat gtcgctgttt ttccggccct ggctattttt ctgaccgtac tggcgttcaa
    51361 tttgttgggc gatggtttac gcgatgcgct ggatccgaaa attaaaggat agttacgttt
    51421 gaatattgct tgaaagggta atcacctcac aggaaattat tgccctaagc aagtgttgta
    51481 actttctgct gattttgtag aatcgggtaa tttggttaaa aagccgcagc aagggactat
    51541 ttttgcagcg gcacagcgtt cagatagtta ttttgttaaa tgtattaaca tgctgagttt
    51601 atacgaaaag ataaagataa ggctgataat tttattttta ttggcagcac tgtcatttat
    51661 tggtcttttt ttcatcatta actatcaact ggtatcggaa cgcgcggtaa aacgtgccga
    51721 tagccgcttt gaacttattc agaaaaacgt tggctatttc tttaaagata ttgaacgttc
    51781 ggccctgaca ttaaaggact cactgtattt attaaaaaat acagaggaga ttcaacgcgc
    51841 cgtgattctg aaaatggaaa tgatgccatt tttagactcg gtgggactgg tacttgatga
    51901 taataaatat tatctctttt cacggagggc gaatgataaa atcgttgttt atcatcagga
    51961 acaagtaaat ggatcgcttg tcgacgagtc agggcgggtt atttttgccg attttaaccc
    52021 atcgaaacga ccgtggtcgg tggcttcaga tgactctaac aacagctgga atccggcata
    52081 caattgcttt gatcgtccgg gtaaaaaatg tatctctttt acgctacgta tcaacggcaa
    52141 agatcacgat ttgttagcgg tagataaaat tcatgtcgat ttaaactggc ggtatttgaa
    52201 cgagtatctt gatcaaatca gcgctaatga tgaagttcta tttttgaaac aaggtcatga
    52261 gatcattgcc aagaatcaac tcgctcgtga aaaactgatt atttataata gcgaaggtaa
    52321 ttataatatt attgattctg tcgatacgga gtatattgca aagacatcag cagtgccaaa
    52381 taatgcattg ttcgaaatct atttttatta tccaggcggt aatttattga acgcatcaga
    52441 taaacttttt tatctaccgt ttgcgttcat tattatcgtg ttgctggtgg tatatttcat
    52501 gaccactcgt gtgttccgtc ggcaattttc tgaaatgacc gagctggtta atacgctggc
    52561 gtttttgccc gactcaacgg atcagatcga ggctctgaaa attcgcgaag gcgatgcgaa
    52621 agagattatc agcatcaaaa attcgatcgc ggaaatgaaa gatgccgaaa ttgaacggtc
    52681 aaataaattg ctctcactga tctcttacga tcaggaaagc ggttttatta aaaatatggc
    52741 gattattgag tccaacaata atcagtatct ggctgtgggg atcatcaaac tgtgtggtct
    52801 ggaagccgtg gaagcggtgt ttggtgttga tgaacgcaat aaaatcgtca ggaaattgtg
    52861 tcagcgaatt gccgagaaat atgcgcaatg ctgcgatatc gtgacattca atgccgatct
    52921 ctatttactt ctgtgtcggg aaaatgtaca gacatttacc cgtaaaatag cgatggtaaa
    52981 cgattttgac agcagctttg gctaccgcaa tctgcgcatc cataagtctg ccatttgtga
    53041 acctttgcag ggggaaaacg cctggagtta cgcagaaaaa ctgaaactgg cgatttccag
    53101 tatccgtgac catatgttct ccgagtttat tttctgtgat gacgcgaaac tcaacgaaat
    53161 agaagagaat atctggattg cgcgtaatat tcgccatgca atggaaattg gcgaactatt
    53221 cctcgtctat caaccgatcg ttgatattaa cacccgcgcc attctgggcg cggaggcgtt
    53281 gtgccgttgg gtttctgcgg agcgggggat catttcaccg ctgaagttca ttaccattgc
    53341 tgaagatatc gggtttatca atgagctggg ttatcagatt attaaaacgg caatgggtga
    53401 attcagacat tttagtcagc gtgcgtcgct gaaggatgat ttcttactgc atattaatgt
    53461 ttcgccctgg cagttaaacg aaccgcactt tcatgagcgt tttaccacca tcatgaaaga
    53521 aaatggcctg aaggcgaaca gcctctgtgt tgagatcact gaaaccgtga tcgagcgaat
    53581 taatgaacat ttttatctca atattgaaca actgcgtaaa caaggggtac ggatatcgat
    53641 tgatgacttt ggcaccggtt tgtcaaacct gaaacgtttt tatgaaatta atccagacag
    53701 cataaaggtg gactcgcaat ttaccggcga tattttcggt actgcgggaa aaattgtgcg
    53761 cattattttc gatctggcac gctataaccg gatcccggtg attgcggaag gcgtagagag
    53821 cgaagacgtt gcgcgcgaat taattaaatt aggatgtgtt caggctcagg ggtatctgta
    53881 ccagaaaccc atgccattct ccgcctggga taaaagtgga aaattagtaa aagagtagtt
    53941 tacgtatgtc cagaatcaat aagttcgtac ttacagtcag tctgctgatt tttatcatga
    54001 tttcagcagt tgcctgcggg atctacactc aaatggtaaa ggaacgggtg tatagcctga
    54061 aacagtccgt tattgatact gcttttgcgg tggcaaatat tgctgaatat cggcgtagcg
    54121 tggcaattga tcttatcaac acgctaaatc ccacggagga acagctgttg gttggtttgc
    54181 gcacagctta cgccgactcg gtttccccct cttatttgta cgatgtcggt ccttatctga
    54241 tttccagtga cgaatgtatt caggtaaagg agttcgagaa aaattattgt gcagatatta
    54301 tgcaggttgt gaagtatcga catgtcaaaa atacaggatt tatctctttt gacggtaaaa
    54361 ccttcgtcta ttacctctat ccggtaactc acaatcgtag tctgatattt ttgcttggtc
    54421 tggagcgttt ttctttactg tcaaaatcgc tggcgatgga cagcgagaac ctgatgttct
    54481 ctctatttaa gaacggtaaa ccggtgaccg gtgatgaata taatgctaaa aacgccatct
    54541 tcaccgtttc ggaagcgatg gagcacttcg cctatttgcc gaccggattg taagtatttg
    54601 cgtataaaaa agatgtttat ttgcgggtct gtacattgat tattttcttt gccgcattgg
    54661 tggcagtgat atcgggtacc agttgcctct atctggtacg cagagtgatt aatcgtggta
    54721 ttgtggagaa agaagccatc attaataacc attttgaacg cgtactggat ggcgggcttt
    54781 tcttttcggc tgccgatgtc aaaaaactct acagtatgta taactcggcg ttcctggacg
    54841 acctgaccaa agcaatgggc agaaaatcct ttgacgaaga tttaaaagcg ctgccggaaa
    54901 aaggcggtta tttgtgcctg tttgacgtcg ataaattcaa aaacattaac gacaccttcg
    54961 gtcatttgct gggcgatgaa gtgttgatga aagtggtgaa aatccttaaa tcacagatcc
    55021 cggtagataa aggtaaaatc taccgcttcg gcggtgacga atttgcggtg atttatacgg
    55081 gtggaacgct ggaagagttg ctatcgattc taaaagaaat cgttcatttc caggtgggaa
    55141 gcattaattt aagtaccagt atcggtgtag cgcattcaaa tgaatgtacc accgtcgaac
    55201 gcttgaaaat gctggcggat gagcggctgt ataagagtaa gaaaaacggc agggctcaga
    55261 ttagctggca gtaatcatta ttcgcaggcc ggacaaatga ttttgcccgg cctgaattaa
    55321 ttaaacccgg ctaccccaca aatcgtactc atcggcgtgc tcgactttca cacgcaggat
    55381 atcacccggc ttaacgttgg tttcaccatt gagataaacc gcgccgtcga tttccggtgc
    55441 atctgccatg ctgcgaccaa tcgcgccttc ttcgtccact tcgtcgataa tcaccagaat
    55501 ttcacggccc actttctctt gcaggcgctc ggcggaaatc tgctgctgca gctgcatgaa
    55561 acggttccag cgttcttctt taacttcttc cggaacctgg tcaggcaggg cattggcgtc
    55621 tgcaccttca accgggctgt atttaaagca gccaacgcga tccagacgcg cttctttcag
    55681 gaagtcgagt agcatctgga aatcttcttc tgtctcgcca gggaagccga caataaaggt
    55741 tgagcgtagg gtcagttccg ggcagatttc gcgccactgt ttgatgcgcg ccagttggcg
    55801 atctacagaa cccgggcgct tcatcagttt gagaatgcgc gggctggcgt gctgcaacgg
    55861 aatgtccaga tacggcagga ttttgccttc tgccatcagt gggatgacgt cgtccacatg
    55921 cggataaggg taaacgtagt gcagacgtgt ccagatcccc agtttcgata actgttcgca
    55981 caggctgacc atgctggttt ttaccggctc gccgttgtgg aagccagtac gatgtttaac
    56041 atcgacgcca taggcggaag tatcctgcga gatcaccaga atctctttaa cgcccgcatc
    56101 taccagacgt ttcgcttcac ttaacacttc gccaatcgga cggctcacca ggtcgccgcg
    56161 catagacgga ataatgcaga aggtgcagcg gtgattacag ccttcagaaa ttttcagata
    56221 ggcataatga cgcggcgtca gtttcacacc ttgttctggc accaggctca ggaatgggtt
    56281 gtgtttcggt tttggcacgt agtgatgaac gtgctccaga acctgctcat agctatgagg
    56341 cccggtgatt tccagcactt tcgggtggac ttcgcggatc tgatcttctt ttgcccccag
    56401 acaaccggtc acaataacct tgccgttttc attcaacgct tcaccaatgg cttccagtga
    56461 ttcttgtacc gcgctgtcaa taaagccgca ggtgttgacg atcaccatgt ccgcatcgtc
    56521 atagctcggt accacgtcat aaccttcagt gcgaagttcg gtgagaatac gctctgaatc
    56581 aacaaggttt tttggacagc caagggaaac aaagccgatt ttcggctggg gagttacttt
    56641 gctcatagct taaaaaatat tcagttacag gaaaggtcag ggcagggatt ctacagagtt
    56701 ctggataaaa tttgtatcgc aatctcattc gctggcggag gcgaaggaaa tgtaaatttt
    56761 gttaattcgg cgtgaagaat tgatcctgga cagcattttg ctcaaaaaat agccatacta
    56821 tttaattgca acaaggctgg aaagaggagg atcgaagtat gttcgttgac agacagcgaa
    56881 tcgatctgct gaaccggttg atcgacgcac gcgttgacct cgccgcatac gtgcaactga
    56941 ggaaggcaaa aggatacatg tccgtcagcg aaagcaatca tctacgagat aactttttta
    57001 aactgaatcg cgaactgcac gataaatcgc tgcggttgaa tcttcatctg gatcaggaag
    57061 agtggagtgc tcttcatcat gctgaagaag cattagcgac agccgcagta tgtttgatga
    57121 gtgggcacca tgattgcccg actgttatta ccgtcaacgc cgataagctt gaaaattgtc
    57181 tgatgagctt aacgctgagt atccagagcc tgcagaagca cgccatgctt gagaaggcct
    57241 gaaaactaag ggggagaaag cgtctccccc ttcatgttta agtttttgta aaaatgaatt
    57301 tgttatctcc tccactgact acgctttaag ccagagtcaa tccggaggcg ttatgcatcg
    57361 acaatccttt ttccttgtgc cccttatttg tctttcttcc gctctctggg cggctcctgc
    57421 aacggtaaat gtcgaagtac tgcaagacaa actcgaccat ccctgggcac tggccttttt
    57481 acccgataat cacggtatgt taatcactct gcgcggcggc gagttgcgtc actggcaagc
    57541 aggaaaagga ttatctgcgc cgctttccgg agttccggac gtttgggcgc acgggcaggg
    57601 cggcctgctg gacgtggttt tagcgcctga ttttgctcag tctcgccgca tctggttaag
    57661 ttattccgaa gttggcgatg atggcaaagc cggaactgct gtgggttatg gccgcttaag
    57721 tgatgatctc tcaaaagtga ccgacttccg caccgtcttt cgccagatgc caaaactgtc
    57781 taccggcaac cattttggcg ggcggctggt attcgacggt aaaggttatc tttttgttgc
    57841 tctgggcgaa aacaatcagc gctcgatggc gcaggatctg gataaattac agggcaaact
    57901 ggtgcgtctg accgacctgg gcgaaatccc ggatgataat ccttttataa aggaatccgg
    57961 tgtgcgcgcc gagatctggt cttatggtat tcgtaatccg caaggaatgg cgatgaatcc
    58021 gtggagtaat gctctgtggc tgaatgaaca tggcccgcgc ggtggtgatg aaattaatat
    58081 cccgcaaaaa ggcaaaaact acggctggcc gctggcaacc tggggaatca actattcagg
    58141 ctttaagata ccggaagcga aaggggagat cgtcgccggg accgagcaac ctgtttttta
    58201 ctggaaagat tcgcccgccg tgagcggcat ggccttctat aacagcgata aattccccca
    58261 gtggcagcaa aaattattta ttggcgcgct gaaagataaa gatgtcattg tgatgagcgt
    58321 caacggcgac aaagtgacag aagatggccg tattttaacg gacagagggc agcgaattcg
    58381 tgatgttcgc actggacccg acggttattt atacgttctc accgacgagt ccagtgggga
    58441 attacttaaa gttagcccac gcaattagct aacgggaatc atcaccactt tgcgcactgc
    58501 ggggcgttca gtgagttgct gataccagcg ttgcagattt ggacgcggtg tccaggtcaa
    58561 gccaacgtta aacaaattgt agatgaacgg tgcgatagcg atatcgccca caccaaactc
    58621 gtcgccagag aaccatttta cttttgccag ttccgcatcg agcagggcaa acagggcgtc
    58681 gcactctttg caactggcat caatggcggc ctgatcgcgc tcttccggtg gtgttctgac
    58741 taatcccatc aggatcccgc gatgagcatt actgagcgtc tggtttgccc agtccatcca
    58801 tttttccgct tccgcacgac gtgccggtga gtcgatccac agacgttttt gcccgtactg
    58861 tgccgccaga tagcggacaa tggcgtttga ttcccaaaga atgagatcac tttcgtcgtc
    58921 acgcaacaac ggcaccagcc cgttagggtt catcgccaga aaatcagcat cgtgatttat
    58981 cccaaactca cggcccgcga gaatttgctc ataaggtagt tccagttctt cgagcgtcag
    59041 caatactttt tttacgttgg ttgaattatt ccgaccccac agcgtaatca tactcacccc
    59101 ttttccgaca ggcagccaca ggctgaacag gactccgatg gtgaagtaaa gttaactttt
    59161 aagcaacagc tggcaaaaaa atgacaccag aagcagcgaa gcggcaggat attgcataaa
    59221 ctttaaaaac tttaccaact tacggtttct ttaagtttgt gtgtgcgtta ttaatcaccg
    59281 aacttatcat acggcgatat aacgtatttt tttgaatgga tactcgggtg gcatttatga
    59341 cgcaatactc ctctctcctt cgtggtcttg cagcgggttc tgcattttta ttcctttttg
    59401 ccccaacggc attcgcggcg gaacaaactg ttgaagcgcc gagcgtggat gcgcgtgcat
    59461 ggattttaat ggattacgcc agcggtaaag tgctggcaga aggcaacgcg gatgagaaac
    59521 tggatcccgc gagcctgact aaaatcatga ccagctatgt ggttgggcag gcgcttaagg
    59581 ccgataagat taaactcacc gatatggtga cggtcggtaa agatgcctgg gcgacgggaa
    59641 atccggcact gcgtggttca tcggtaatgt tcctcaaacc gggcgatcag gtttcggtgg
    59701 cagacttgaa caaaggtgtg attatccagt ccggtaatga cgcctgtatt gcgctggccg
    59761 attacgttgc cgggagccag gagtcattta ttgctttgat gaatggttat gccaaaaaac
    59821 tgggtctgac caacactacc ttccagacgg tgcacggtct ggatgcgccg gggcagttca
    59881 gtaccgcgcg cgatatggca ttgctgggta aagcattgat ccacgatgtg ccggaagagt
    59941 acgccattca taaagagaaa gagttcacct tcaacaaaat tcgtcagcct aaccgtaacc
    60001 gtctgctgtg gagcagcaat ctgaatgttg atggcatgaa gacaggaacc acggcaggcg
    60061 cgggatataa tctggttgct tcggctaccc agggcgatat gcgtttaatc tccgtagtgc
    60121 tgggggcgaa aactgaccgt atccgtttta atgagtctga gaaattattg acctggggtt
    60181 tccgcttctt tgaaaccgtg acgccaatta aacctgatgc cacctttgtg actcagcgcg
    60241 tctggtttgg tgataagagc gaagtgaatc tcggggcagg cgaagcgggc tcagtgacca
    60301 taccgcgtgg gcagctgaaa aacctgaagg cgagctacac gttaacggaa ccgcagctta
    60361 ccgcaccgct gaaaaaaggt caggttgtcg ggaccattga tttccagctt aacggtaaat
    60421 ccattgagca gcgtccgctg atcgtgatgg aaaatgtgga agagggcgga ttctttggtc
    60481 ggatgtggga tttcgtgatg atgaaattcc atcagtggtt cggcagctgg ttctcttaat
    60541 cttctgataa ccggatggcg cgaaacgtca tccggttata cgtcattaat acatcaactt
    60601 aatacggtgc gtctgcgcgt acttcacata ctcatcttcc gggcaacaat cactcaccac
    60661 aatatcaaag cgtttcaggt cacccatgcg cgccggacgc accttgccaa atttactgtg
    60721 gtcgacaacc agcacatgct tttgcgccat cgacatggcc cagtgtttta ccggcaactc
    60781 ttcaagatta aaacaggtag cgcctttact gacatgcacg cccgccgcgg aataaaaagc
    60841 gatatccggg caaaaattat tcaacgtttg ctgaaaatcg atgggtttga aaatggcgtt
    60901 gctggcgtga aattcaccac cgcacagaaa tgcgcggcaa tggggtttct ctttcagcgc
    60961 cagaaaggta tttagcgaat aacaaacggc ggtaaaaggg atttcattat caatcgcttc
    61021 aataatccac ggcgtggtgg tgccacagtc aaaaaagaga gtctgatcgg gttctaccag
    61081 tgtcgcagcc agttttgccg cccggcgttt ttcttccacc aggcgggatt tttgatcgct
    61141 taacaggtaa tggctggcac tgcgcggttc cagaacaata tagccgccga gcaaaacgac
    61201 gggcgcactg tggttgttca gatcgcgacg aatcgtcatc tccgaaaccc caagcagggc
    61261 ggcggcgtct ttaagatgta acttatcgct gcgttttaat tcttgcagca gctgcccgat
    61321 acgctcttcg cgacgtgttt ccataatccc tctgaatagt tattgaagcg agccgctcaa
    61381 tactacactt tttagcagag atcagtcacg cacccagcct ttgcggatcg gtaatgcaaa
    61441 acagaagcga taccacgatt gcagacgttg atagagttta tgccccatcg cttgccagat
    61501 aatctgagcg ctaaggcagc caatcatacc tgccagcaat ccacctagca tatccagcgg
    61561 ccagtgcacg ccaagataaa cgcgcgacca ggcaatgacg acggccagca ccattaaaag
    61621 tgagccggac cacaggcgat gccagcataa aaatgccagt gcaaaggtga aaatcaccgt
    61681 accgtgatcg cttgggaatg agtcatccgc cgcatgatgc aggaagttat agccgatatt
    61741 ttcgacaaag ggtcggtcgt gcggaaaaag atgtcccatc gtccaggaca caaacaggct
    61801 gaccgccagc gcgatagcga tttttatcac cagttgccgt tgtgctgtaa gcccccacaa
    61861 ccaaagtacc gcggccagca gcggcaccac ggtaatcaaa tctttagcaa taaaaatcgc
    61921 caacgagatc atccacggag ccgagtctgg cgtcgcgtta ataagagaga atagagagag
    61981 attcaaattt tccagcataa cttcccgacg caaagtgatt aaaagggagc caatacaggc
    62041 aagtcgttga gaataaagta caggttaaac tgggtaaagc ggcatcgtct tatttccctc
    62101 aagcggcctg tttacggtgg gtgattgtaa cgggcatagg ttaaataaaa cttaaagaaa
    62161 gcgtagctat actcgcaata atgtaagaat gtgcttaacc gtggtttcag ctacaaaatt
    62221 cgctttctcg ttagctgcgc ttttattaaa ctctgcgcga ttattattgg cgaagaaatt
    62281 gcatgcaaaa taaattagct tccggtgcca ggcttggacg tcaggcgtta cttttccctc
    62341 tctgtctggt gctttacgaa ttttcaacct atatcggcaa cgatatgatt caacccggta
    62401 tgttggccgt ggtggaacaa tatcaggcgg gcattgattg ggttcctact tcaatgaccg
    62461 catatctggc gggcgggatg tttttacaat ggctcctggg gccgctgtcg gatcgtattg
    62521 gtcgccgtcc ggtgatgctg gcgggggtgg tgtggtttat catcacctgt ctggcaatat
    62581 tactggcgca aaacattgaa caattcactc tgttgcgctt cttgcagggc ataagcctct
    62641 gtttcattgg cgctgtggga tacgccgcaa ttcaggaatc cttcgaagag gcggtttgta
    62701 tcaagatcac cgcgctgatg gcgaacgtgg cgctgattgc tccgctactt ggtccgctgg
    62761 tgggcgcggc gtggatccat gtgctgccct gggaggggat gtttgttttg tttgccgcat
    62821 tggcagcgat ctcctttttc ggtctgcaac gagctatgcc tgaaaccgcc acgcgtatag
    62881 gcgagaaact gtcactgaaa gaactcggtc gtgactataa gctggtgctg aagaacggcc
    62941 gctttgtggc gggggcgctg gcgctgggat tcgttagcct gccattgctg gcgtggatcg
    63001 cccagtcgcc gattatcatc attaccggcg agcagttgag cagctatgaa tatggcttgc
    63061 tgcaagtgcc tattttcggg gcgttaattg cgggtaactt gctgttagcg cgtctgacct
    63121 cgcgccgcac cgtacgttcg ctgattatta tgggcggctg gccgattatg attggtctgt
    63181 tggtcgctgc tgcggcaacg gttatctcat cgcacgcgta tttatggatg accgccgggt
    63241 taagtattta tgctttcggc attggtctgg cgaatgcggg actggtgcga ttaaccctgt
    63301 ttgccagcga tatgagtaaa ggtacggttt ctgcggcgat gggaatgctg caaatgctga
    63361 tctttaccgt cggtattgaa atcagcaaac atgcctggct gaacgggggc aacggactgt
    63421 ttaatctctt caaccttgtc aacggaattt tgtggctgtc gctgatggtt atctttttaa
    63481 aagataaaca gatgggaaat tctcacgaag ggtaaaaaat gcctgattgc tttgtgcgat
    63541 caggcattct cgaattaatg gtgatggtcg tcaatctggt gttcgataac catcccttca
    63601 cctacgctgg caagatggcg aacataagga tgcgggcggt aagccggagc tggcgcagga
    63661 gccacataga cggtttgcgg cacagtcgcg acgctgaccg cttgtggaac gctgactgaa
    63721 tcaggaacca caaccacttt gtatccactc ggcacatcga cggtgacact ttgcgccatc
    63781 gccacaccag aaaagcccat taaaagcgcg cccaacagta gacaattttt cataaacatt
    63841 tcgcctgaaa taatgcagcg ggttaatttc cgcgaattat gcagccttca ttcaggtatt
    63901 gtgttgccgg gcgtgctgcc gtttttattg gtcaaatggc gcttcgtgtt taagcacttt
    63961 atcgatcaca tccagtacgc cttcacggtt attggagcct gcccggtatt ttgccgctgc
    64021 gacgaccgcg ctgccggcat tttccattgc aaaactaaag cctgcctgac gcagcatctc
    64081 aatatcgtta ccgccatcgc caaagactac cacttcgctg tcgtctattc cccataattt
    64141 ctgcagttgg cgaaggccat tggctttatg tacgccgggg ataatcagat cgatgctgcc
    64201 gttgccggtg tggactggca ccataatatc gccgatggcc tcatgtaatg ctttttgtac
    64261 ttgtggaatc agttcatcgg aaagattcag gccaaactta aagaaaatat cttctaagtt
    64321 gtcaaagtta tcgacgtatt ccagacggtg ataatacatt tccgccaccg ttttcatggc
    64381 atcgtcatat tttttgagtg tataggcgct attttttccg caggcaataa tttccacttc
    64441 cgggcgcgtc agcaaatgtt ccacgacagt agcaaacgca tccttcgaca gctcgccatt
    64501 aaaaacatct ttgccttcac tcactaccca gccgccgttt tcagccacaa aggcaatttc
    64561 attcgcaatt tcagggaaga aagagatcaa ctgataatat tgatttccgc tggcgaccac
    64621 aaagcgaatg ccttgtgctt tcatttgctg atactgagcc ataaaccgct cacggttata
    64681 ggttttttga tcgcttaaga aagtaccatc catgtctacc gcaattaatt taatgctcat
    64741 caactattct ccatcgccgt ctgcgttttg gtatcgggtt tggcgacggc tttcgcgaca
    64801 atagccgcga gaataaccag cgccagtaca accagcattg cactacgtaa tccataatgt
    64861 tcgccgagat agcccagcag cggcggcccg acgaggaaag ccagataacc ggtcgttgct
    64921 accacactga cgcgggttgg tgcatcgggg ccggtatcgc tggcggcaga aatggtcagc
    64981 gggaagccca gcgaggcacc cagtccccag agtacaacag acaccccagc gacccaggcg
    65041 ctatcgacaa aaataatcag cccaataccc aacgctccca ttagtgcact ggcccgaacc
    65101 acggcaacgc gactgtaacg gtcgatgaac caaccgccgg tgaagcgtcc aacggtcatc
    65161 cccagggtaa aaccggcata aatcagtgag ccggaagtgg ggctaaaacc gtgaccatca
    65221 accattaata agggtaacca gtcgttggca gaaccttcgg caaaggccat cgccagcact
    65281 acaacaccaa tcaacagcaa ctggatatcg cgataaaaag gaatgctttt ttgccatgct
    65341 gggtgccatc ggcagcattt ttgcccgtac cgtcagggat tgcctgaatg gcgatataaa
    65401 taggcgcgat acctaccagc gccgccaata aaatgtgcac cgttgccgga acgccaaagg
    65461 ccgtcagtgc catcccgaca cctgcgcctg ccagcgtgcc caggctataa aaaccgtgca
    65521 tcatcggcaa aaccgtttta ttcatttctc gctcaacggc agcaccttca acgtttatcg
    65581 ccacttccgc agaaccaaaa cttgccccaa agacgccgag accaacggca aagagcaggg
    65641 gcgatgtcag ccagagtgcc agacttaata tcatcatccc gatcaatgcg caggacatcg
    65701 tgaccaggat gacattacgt gtcccaaagc gtttcactaa ccacgccgag cagagaatac
    65761 cgctcatcga accgatcgac agaccaaaga gaacaccgcc catttcagcg atcgagacag
    65821 agagaatatc gcggatagca ggcgtacggg ttgcccagga cgccattaac aggcctggca
    65881 aaaagaagaa cataaacagc gcccaggttc ggcgtttcaa tgcattacgt gaagaattta
    65941 cggtcataga tcacgtcaaa ataagaagag ggaagacaac attagcaagg ttgtgtacat
    66001 ttgtacacaa ttgcagacag aggaaatgac atgcgtcgcg ctaacgatcc gcaacggcga
    66061 gaaaaaatta tccaggccac gctggaggcg gtgaaacttt acggaataca tgctgttacg
    66121 caccgcaaaa ttgctaccct tgccggggta ccgttgggat cgatgaccta ctatttttca
    66181 ggaattgatg agttgttgct ggaggcgttc agcagtttta ctgagattat gtcccggcaa
    66241 tatcaggcat tttttagcga tgttagtgat gctcaaggcg catgccaggc tatcaccgat
    66301 atgatctaca gcttacaggt tgcaacgccg gataatatgg agctgatgta ccagctctac
    66361 gcgctggcta gccgaaaacc gctattaaaa acggtaatgc aaaactggat gcagcgcagt
    66421 cagcaaacgc tcgaacaatg gtttgaaccc ggaaccgccc gcgcgcttga tgcgtttatt
    66481 gaggggatga cgctgcattt tgtcaccgac cgtaagccgc tattgcgcga ggagattttg
    66541 aggatggttg agagggttgc agggtagtag ataaatttta gataacaaaa aacccatcaa
    66601 ccttgaaccg aaatggcggg gttgatgggc tccacaaaat ggggacatca aagaaaagca
    66661 gtggcactaa ttaagactga tgccctgcgg aaaagttctg cggttgtgca aaaaaatttc
    66721 attttcaggg caacttcagt tttatcctaa tcctggccat accatgacga tgattgtccc
    66781 tgccagcgtc agcaggacgt tggcgattgc gtaggtgccc gcatagccca gcgcagggat
    66841 gttactgcga gctgtatcac tgatgatctc cattgccggc gcgcaggtgc gtgcgcccat
    66901 cattgcgccg aacaacagtg cgcggttcat tcgcaataca taagcaccga acaagaaaca
    66961 gataaccacg ggcacaagac tgacaattaa tccggcaatc aacatctgac cgccaatcgc
    67021 gcccaggccg ttattaatac cgctaccggc gctcagacca acgcctgcca taaacaccat
    67081 caagccgaac tctttcacca tgcttaatgc accttgcgga atgtaaccga aggtcgggtg
    67141 gttagcacgc ataaagccca gcataattcc ggcgaataac aacccggcag cgttccccat
    67201 gccgaaactg aatgtgctga actggaaggt gatcatcccg atcatcagcc caataacaaa
    67261 gaaggcgcaa aatgccagca ggtcagtgac ctggctgtga atcgagataa agccgatgcg
    67321 atcggcgatg gtttttacgc gacgggcatc accgctgact tgtaaaacgt cacctttgtt
    67381 aagcacgacg ttgtcatcta tcggcatctc aatctggcta cgaatgacgc ggttaaggaa
    67441 gcaaccgtga tcggtcaact tcagttgtgc gagacgttta cctacagcgt tatggttttt
    67501 aacgaccact tcttcagtga cgatacgcat gtcgagaagg tcacgatcga aaacttcttt
    67561 accgttacgg aagctgggat cgagtcgggc atgggcgtcg ggatagccta ccaacgctat
    67621 ttcatcgccc atttgtagca cggcatcacc gtctggattt gccagaatcc cgttacgtcg
    67681 aatacgttca atgtagcagc cggtttgtcg ataaataccc agttcacgca gatttttgcc
    67741 gtcggtccag gccaccagct ccgggccgac gcgataggcg cggatcaccg gtaaataaac
    67801 cttacggttg gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg cgctggtctg
    67861 taagtcctga tgctgcaatt tcggcaagta acgcgcacca acaatcaaac tcaccagacc
    67921 gattaaatag gttaaggcat acccgaggct cagattatcc agtgccagtg agagctgcct
    67981 gctttccatg ccggaatgac gcagtgtatc gccagcaccg accagaaccg gtgtcgacgt
    68041 catagagcct gctaacatac cggccgtcag gccaatatcc cagccaaaca gcttacctaa
    68101 ccctaaggcg atcaccagcg cactgccaac catcaccagt gctaacatta ggtaattttt
    68161 cccatcgcga aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga cgcagaaaat
    68221 aaacagcata aagccaagat taagcgcgtc ggtgttaatg ctgaaatgtt gttggcctaa
    68281 taacagtgat acgaccaaaa cgccaatgga attacccagt tggatcgaac caagtcgtaa
    68341 ctttccgaga catagcccaa gcgcgaggac cacaaataat aacagaatgt aattcccatt
    68401 taacaattcg gcgacgttta tattcacgga ggctaacttc ttgtttacta gtaagctgtt
    68461 gaaagaaatg gtaatttacg ataatgtttt ttaccagaat tcagggcgca gattcattca
    68521 gcgcacctaa acgatagtaa agtaacaata tattttacta gtgtaatcac attaggtatc
    68581 aacggctata tgaattgcgt tggcctatat tagcatggaa tgcgaagcgg ctttatctta
    68641 ctgaacgcca cactggcgaa aaatgtgttc gatagacgca gtgtcaggag gaacgagtga
    68701 aacataaaca acgttgggcg ggggcaatct gctgttttgt cctcttcatt gtggtgtgcc
    68761 tttttctggc gacgcacatg aaaggcgctt ttcgggctgc cgggcatcct gaaatcggat
    68821 tgctgttttt cattcttcct ggagcagttg ccagtttctt ttcacagcgt agagaagtcc
    68881 tgaaacctct atttggcgca atgctggcgg caccctgttc aatgcttatt atgcggctgt
    68941 ttttttcacc gacccgctca ttctggcaag agctggcatg gttactaagc gcggtgttct
    69001 ggtgtgcgct gggggcactg tgtttcttat ttatcagtag tttgtttaaa ccacagcaca
    69061 gaaaaaatca gtaaagccct caacgcgagg gcttgtcaga cgatcaggcg tccagatttt
    69121 ctttcaccca tgcagcaaaa tcggtatagc cgccgatatg ttgctgatcg acaaaaatct
    69181 gcggcacggt ttctacgggt ttacctgcct tttgttgtag atcttcttta gtgatccctt
    69241 ccgcacgaat atctacatac tgatactgaa aatcatcgcg ttcattgctc aatttctcag
    69301 ccagatcttt tgcacgcaca cagtaagggc aacccgaacg accaaaaata acggtttgca
    69361 ttatttctct cctcatagat ttatgcctgt aatgatcacg ctaaaatgta ttcgctgaaa
    69421 gtaggtttaa cctgttgcat taattgctaa aagctataac tgttaaacac aatacagtga
    69481 aaagttttag actgaaggct cactttgcag agggaagcgt atgcgcgcga tcggtaaatt
    69541 gcctaaaggc gtgttgatac tggaatttat cggaatgatg ctactggcgg tggcgctgct
    69601 gtcggtaagc gactccctgt cgctgcctga gccattttct cggccagaag tgcagattct
    69661 gatgattttt ctcggtgttt tgctcatgct tcccgctgcg gtggtggtta ttcttcaggt
    69721 ggcaaaacgt cttgccccac agctgatgaa ccgtccaccg caatattcac gttcagaaag
    69781 agaaaaagat aatgacgcca accattgaac ttatttgtgg ccatcgctcc attcgccatt
    69841 tcactgatga acccatttcc gaagcgcagc gtgaggcgat tattaacagc gcccgtgcga
    69901 cgtccagttc cagttttttg cagtgcagta gcattattcg cattaccgac aaagcgttac
    69961 gtgaagaact ggtgacgctg accggcgggc aaaaacacgt agcgcaagcg gcggagttct
    70021 gggtgttctg tgccgacttt aaccgccatt tacagatctg tccggatgct cagctcggcc
    70081 tggcggaaca actgttgctc ggtgtcgttg atacggcaat gatggcgcag aatgcattaa
    70141 tcgcagcgga atcgctggga ttgggcgggg tatatatcgg cggcctgcgc aataatattg
    70201 aagcggtgac gaaactgctt aaattaccgc agcatgttct gccgctgttt gggctgtgcc
    70261 ttggctggcc tgcggataat ccggatctta agccgcgttt acctgcctcc attttggtgc
    70321 atgaaaacag ctatcaaccg ctggataaag gcgcactggc gcagtatgac gagcaactgg
    70381 cggaatatta cctcacccgt ggcagcaata atcgccggga tacctggagc gatcatatcc
    70441 gccgaacaat cattaaagaa agccgcccat ttattctgga ttatttgcac aaacagggtt
    70501 gggcgacgcg ctaaaaccgc cacgtcgatg tatgatacgc gggcttttga ccaggtctga
    70561 cagagaggtg cagggtgaaa attgccatat tgtcccggga tggaacgctc tattcgtgta
    70621 agcggctgcg tgaagccgct atacagcgcg gtcacctggt tgaaattctt gatccgcttt
    70681 cttgctacat gaacataaat cctgcggcgt cttctattca ctacaaaggc cgcaagttac
    70741 cccattttga cgcagtgatc ccgcgtattg gcaccgccat taccttttat gggacggcgg
    70801 cactgcgcca gttcgagatg ctggggagct atccgctcaa tgagtcggtc gccattgccc
    70861 gggcgcgtga caaattgcgt tccatgcaac tgctggcgcg tcagggcatc gacctgcctg
    70921 tcacgggcat tgcgcattcg ccggatgata ccagcgattt aatcgacatg gtcggtggtg
    70981 cgccgctggt ggtcaagttg gttgaaggca cgcagggaat tggcgtcgtg ctggcggaga
    71041 cgcgtcaggc ggcggaaagc gtgattgacg ctttccgcgg tctgaacgcg catattctgg
    71101 tgcaggaata tatcaaagag gcgcaagggt gcgatatccg ctgtctggtt gttggtgatg
    71161 aagtggtcgc tgcgattgaa cggcgggcga aagagggcga ttttcgttcc aatttgcatc
    71221 gtggcggcgc ggcaagcgtc gccagtatca caccacagga gcgtgaaatc gcgataaaag
    71281 ccgcgcgaac gatggcgctg gacgttgctg gtgtggatat tctgcgtgct aatcgcgggc
    71341 cgttggtgat ggaagtgaat gcgtcgccgg ggctggaagg aatagaaaaa accactggta
    71401 tcgacatcgc gggtaaaatg atccgctgga tcgaacgcca cgctacgaca gaatattgcc
    71461 tgaaaacggg tggttagtcg caatcacatt actgatcatg gttttgcctg cgctttttgc
    71521 gtaagctgtg ccggtctttt tatcgaaaga ggttgtacaa aattatgaca tcgctggtcg
    71581 ttcctggtct ggatacgctg cgtcaatggc tcgatgacct ggggatgagt ttttttgaat
    71641 gtgataactg tcaggctctg catctgcccc atatgcagaa tttcgacggt gtctttgatg
    71701 ccaaaatcga tctgatcgat aacacgatcc tgttttctgc catggcggaa gtccgacctt
    71761 cagccgtatt gccgctggcg gcggatttat ctgccatcaa tgccagttcg ctgaccgtga
    71821 aagcatttct tgatatgcag gatgataatc tgccaaagct ggtggtttgc cagtctatat
    71881 ccgtcatgca gggcgtaacc tacgagcagt ttgcatggtt cgtgcgtcag agcgaagagc
    71941 agatttcgat ggtcattctt gaagctaatg cccatcaact gctgttaccg actgacgatg
    72001 aagggcaaaa caacgttacc gaaaactatt tcctccactg ataactcctt tcgagcacgc
    72061 agtcgctggt gcagtgactg cgcgctgcaa aattatctgc tgtttttaac tttttcttaa
    72121 agattatttc acttctcttg tgccgatttg gctttatcac atagagcaaa tacgcataaa
    72181 aatttgttaa ataccgtttt ttaatccgag ctatagtctc aaaccctggc taaagttatt
    72241 cttgcgatgc ttttatatag cgagcagtgc tggccgggag agagttctct tttcttacac
    72301 cgcgccgata aaaaatatgc acgtttattg catatctttt agtgtgacaa cttttgttcg
    72361 tttgttaacg aactttcaga aggaaagaga tatgaccgcc ttaaataaaa aatggctttc
    72421 gggtctggtt gcgggtgctc tgatggccgt ctctgtcggc acgctcgcgg ctgaacaaaa
    72481 aacactccac atttataact ggtctgatta tatcgccccg gacacggtgg ccaattttga
    72541 aaaagaaacc ggtattaaag tcgtctacga tgttttcgac tctaacgaag tactggaagg
    72601 caaattaatg gcgggtagca ccggctttga cttggtggtt ccatctgcca gctttcttga
    72661 acgccagttg actgcgggag ttttccagcc gctggacaaa agcaaattgc cggagtggaa
    72721 gaatctcgac ccggagctgc tgaagctggt cgccaaacac gatcccgaca ataaatttgc
    72781 tatgccctat atgtgggcga cgaccgggat tggctataac gttgataaag ttaaagctgt
    72841 gctgggcgaa aacgcgcccg tcgatagctg ggacttgatc ctcaaacctg aaaatctgga
    72901 aaaactgaaa agctgcggtg tctctttcct ggatgcgcca gaagaagttt ttgctaccgt
    72961 gttgaattat ctcggcaaag atcccaacag cactaaagcg gatgattaca ccggaccggc
    73021 aaccgatctg ctgttaaagc tgcgcccgaa cattcgttat ttccattcat ctcaatacat
    73081 taacgacctg gcaaacggcg atatttgcgt cgctatcggc tgggcaggtg atgtctggca
    73141 ggcgtcaaac cgcgcgaagg aagcgaagaa tggagtgaat gtctcgttct cgattccaaa
    73201 agaaggggcg atggcgttct ttgatgtatt cgccatgcct gcggatgcca aaaacaaaga
    73261 cgaagcctat cagttcctga attacctgct gcgcccggat gtagtagcgc atatttccga
    73321 ccatgtgttc tatgccaacg ccaataaagc agccacgccg ctggtgagtg cggaagtccg
    73381 tgataacccg ggtatttatc cgcctgcgga tgtccgtgcg aagctgttca ctctgaaagt
    73441 gcaggatccg aaaatcgacc gtgtgcgcac ccgcgcgtgg accaaagtga agagcggaaa
    73501 ataatccgca gccttagatg ccgggcgggc gcaccacacc cgccggcaat tcgcaccatc
    73561 atggtgcgct tgcacacatt caatgccgga gagcagccgt gaatgacgct atccctcgcc
    73621 cgcaggcgaa aacccgtaag gcgctgacgc cgctattaga aatccgcaac ctcaccaaat
    73681 cctacgatgg tcagcatgcg gtcgatgatg tcagcctgac catctacaaa ggtgaaatct
    73741 tcgcgttgct gggcgcatcc ggctgtggca agtccacgct gctgcgtatg ctggcaggtt
    73801 ttgaacaacc ttctgccggg cagataatgc tcgatggcgt cgatttgtca caggtgccgc
    73861 cttacctgcg ccccatcaat atgatgtttc agtcttacgc gctgtttccg catatgaccg
    73921 tggagcagaa catcgccttt ggcctgaaac aggacaaact accgaaagcg gaaattgcca
    73981 gccgggtcaa cgagatgctt gggctggtgc atatgcagga gttcgccaaa cgcaaaccgc
    74041 atcagctttc cggcggtcag agacagcgtg tggcgctggc ccgaagcctt gccaagcgcc
    74101 caaaacttct gctgctcgac gaaccgatgg gcgcgctgga taaaaaattg cgtgaccgga
    74161 tgcagcttga agtggtggat attctggagc gtgtcggcgt gacctgtgtg atggtcactc
    74221 acgatcagga agaggcgatg acgatggcgg gacgtatcgc cattatgaat cgcgggaaat
    74281 ttgtccagat tggcgagcca gaagagatct acgaacatcc aaccacccgc tacagtgctg
    74341 aatttatcgg ttcggttaat gtctttgaag gtgtactcaa agagcgtcat gaagatgggc
    74401 ttgtgcttta ttcgcctggg ctggtgcatc cattgaaagt tgatccggat gcttctgtag
    74461 tggataacgt gccggtgcat gtggcgctac gcccggaaaa aatcatgctt tgcgaggaac
    74521 ctcccgctga cggttataac tttgcggtag gtgaggtgat gcacattgcc tatctcggcg
    74581 atctttcggt gtatcacgtt cgtctcaaga gcggacagat gatcagcgct cagctacaaa
    74641 acgctcatcg atatcgcaaa gggttaccaa cctggggcga tgaagtacgt ttgtgctggg
    74701 aggtagacag ttgtgtggtg ctgacggttt aaggagcaaa gatgagtaca cttgaacctg
    74761 cagcccagcc gaaaccgccg ggcggattaa ggctgtggct gtcgcagttg caaatgaagc
    74821 acgggcgcaa actggtgatt gcgttgccgt atatctggtt gatcctgctg tttctgctgc
    74881 catttctgat cgtctttaaa ataagtctgg cggagatggc gcgcgccatt ccgccttata
    74941 ccgaactgat ggagtgggct gacgggcaac tgtccatcac tcttaatctc ggtaattttc
    75001 tgcaactgac tgacgatccg ctctatttcg atgcttatct ccagtcgcta caggtggctg
    75061 cgatttcgac attttgctgt ttactgatcg gctatccgct ggcgtgggcg gtggcgcaca
    75121 gtaagccttc gacccgtaat attttattac tactggtgat cctgccgtcg tggacctcgt
    75181 ttctgatccg cgtttatgcc tggatgggaa tattaaaaaa caacggtgtg ctgaataatt
    75241 ttctgctgtg gctgggggtt atcgatcaat cgctgaccat tcttcatacc aatctggcag
    75301 tttatattgg cattgtttac gcctacgtgc cgtttatggt gctgccgatt tataccgcgt
    75361 tgattcgtat tgattcttcg ctggtggaag cagcgctgga tctcggtgca cgaccgctga
    75421 aaacgttctt tactgtgatc gtgccgctga ctaaaggtgg gattattgcc ggatcgatgc
    75481 tggtgtttat cccggctgtg ggcgagtttg tgatcccgga actgctcggt ggcccggaca
    75541 gcatcatgat cgggcgcgtg ctatggcaag agtttttcaa taaccgcgac tggccggtgg
    75601 cctcggcggt agcgatcatc atgttgctgc tgctaattgt gccgataatg tggttccaca
    75661 aacaccagca aaaaagcgtg ggagaacacg gatgaataat ttaccggtag ttcgttcgcc
    75721 ctggcggatt gtgattttgc tgctgggctt tacctttctc tacgcgccaa tgctgatgct
    75781 ggttatctat tcgtttaaca gctcgaagct ggtgacggtg tgggccggct ggtcaacgcg
    75841 ctggtatggt gagttattgc gcgatgacgc gatgatgagt gcggttggtt taagcctgac
    75901 aattgctgcc tgtgcggcaa cggcggcggc gatcctcggg actattgcag cggtggtgct
    75961 ggtgcgtttt ggcaggtttc gcggatcaaa tggttttgcc tttatgatca ccgcgccgct
    76021 ggtgatgcca gatgtcatca cgggcttgtc gctgttgtta ttattcgtcg cgcttgctca
    76081 tgccattggc tggcctgcgg accgcggtat gctcaccatc tggctggcgc atgtcacttt
    76141 ttgtacggct tatgtggcgg tcgttatttc ttcaagattg cgggaactgg atcgctcgat
    76201 agaagaagca gcgatggatc tcggtgcgac gccgctgaaa gtgtttttcg tcattacgct
    76261 accgatgatc atgcccgcga tcatttctgg ctggttactg gcttttactt tgtcgcttga
    76321 tgatctggtg atcgccagct ttgtttccgg gccgggagcc accacgttac cgatgctggt
    76381 cttttccagc gtgcggatgg gagtgaatcc ggaaatcaac gcccttgcaa cgttaattct
    76441 cggtgcggtc ggaattgtcg gatttatcgc ctggtatctg atggctcgcg cagaaaaaca
    76501 gcggatacgc gatatccagc gtgcaagaca tggctgaaga cactaaaatt tgccaacctg
    76561 gctacataat gccgcgcatg ccgcggcatt gttttcatgg aagacgaaac gttgggattt
    76621 tttaagaaaa catcttcatc tcatgctcgc ctgaatgtgc ctgcgctggt gcaggtagcg
    76681 gcgctcgcca ttattatgat ccgtggcctc gacgtgctga tgattttcaa tacgctgggc
    76741 gtgcgcggta ttggcgagtt cattcatcgc agcgtacaaa cctggagctt aacgctggta
    76801 tttttaagca gtctggtgct ggtttttatc gagatctggt gtgcgttttc attggtgaaa
    76861 gggcgtcgct gggcgcgctg gctatatctg ctgacacaaa tcaccgccgc aagttacttg
    76921 tgggcggctt cgctggggta cggttatccg gagctgttca gcattcccgg tgaatcaaaa
    76981 cgtgaaatct tccatagcct gatgctgcag aagctgccgg atatgctcat cctgatgctg
    77041 ctgttcgttc cctcgaccag tcggcggttc ttccagttgc aataatgtgt ataatcgtcg
    77101 cccctgatga tgtgaaggtc aatgtatgca gtgcgcactt tacgacgcgg gtcgctgtca
    77161 ttcctgtcag tggataacgc agccgattcc agagcaactc tccgctaaaa ccgccgatct
    77221 taaaaatctg ctcgccgatt ttccggttga ggaatggtgc gcgccggtgt caggcccgga
    77281 acaagggttt cgtaataaag ccaaaatggt ggtgagtggt agcgttgaaa aaccactgct
    77341 cggtatgctg catcgcgatg gcacgccgga agacctttgt gactgcccgc tttatcctgc
    77401 ctcttttgcg cccgtttttg cggcgctaaa accgtttatc gcccgtgcgg ggttaacgcc
    77461 ctacaacgtg gcgcgtaagc gtggcgaact gaaatacatt ctgctgactg aaagccagag
    77521 tgatggcggc atgatgctgc gctttgtact gcgttctgat accaagctgg cgcaactgcg
    77581 taaggcgctg ccgtggttac acgaacaact accgcagctg aaagttatta ccgtcaatat
    77641 tcagccggta catatggcga ttatggaagg ggagacggag atctacctga ccgaacaaca
    77701 ggcactggcg gagcgtttta atgacgtacc gctgtggatc cgtccgcaaa gtttcttcca
    77761 gactaatccg gcggtcgcca gccagttgta cgccaccgcg cgcgactggg tacgacagct
    77821 gccggttaaa catatgtggg atctgttctg cggtgtgggg ggctttggtt tacactgcgc
    77881 gacgcctgac atgcagttaa ccgggatcga aattgcgcca gaggccattg cctgtgcaaa
    77941 gcagtcagcc gctgaactgg gcttaactcg tttacaattt caggcgctgg actccactca
    78001 gtttgccact gctcaggggg aggtgccgga gctggtgctg gttaacccgc cgcgccgcgg
    78061 cattggtaaa ccgctgtgtg attatctctc aacgatggca ccgcgtttta ttatctactc
    78121 cagctgtaac gcccaaacca tggcgaaaga tatccgcgaa ctgcctgggt ttcgcatcga
    78181 acgggtacag ctttttgata tgttcccgca caccgcgcac tatgaagtgc tgacgctgct
    78241 ggtgaagcaa taaaaaagcc gcaggtgcgg cttcagattg ctgatcaggt gcgctttgtt
    78301 catgccggat gcggcgtgta cgccttatcc ggcctacaaa atcatgctaa atcaatacat
    78361 tgcagagttg atgtgggccg gataagcgca gcgcatcagg caatcttact tttgtcatca
    78421 gtctcaatta ctgcggaaac cactggtcat tgattttctg atatgtaccg tcagctttaa
    78481 ttgctgccag cgcgttattc agtttttcca gcagagcttt gttatccgga cgtacagcga
    78541 tgcccagacc ggtgccgaag tattgcggat cggtcacttt ttcagtagca acgcccagtt
    78601 gcggattggt tttcagccat tcgtttacca ccgctgtgtc accaaatacc ccatcaatac
    78661 gaccattttt cagatcgata aaggcattct gataactgtc ataggagaca gttttcactt
    78721 cggggtgctg atcctgaata tatttctggt gcgtggtgcc gttttccatc ccaatacgtt
    78781 tgcctttcag atcggcaaac gttttgtagg tatctttttt ggcaatcacg acggctgagt
    78841 tttcatagta gggcgtggta aacgatacct gcttgctacg ctcgggcgtg atatccatac
    78901 cggagattac ggcgtcatat tttctgaatt tcagtgacgg gatcaggctg tcgaacgcgt
    78961 gattagtaaa agtacattct gcctgcattt gtttgcacaa ggcttttgcc agatcgatat
    79021 caaagccgac aatctcatta ttagcaccta tagattcaaa gggtggatag gtggctgaaa
    79081 cgccaaaatt gattttctct gcggcagaag caccgaaagt aaaggaagca agtaaagcgg
    79141 caagaactaa ctttttcatg atggaactcc cgtctgtcaa tcttatgatt tttggccgtg
    79201 tctgcggcat gggataacaa tgccatcaag tgaatttata tgcaataaat atgattaaat
    79261 aatttaaatg aaataaaaaa agacggataa cttagtgagt tgtccgtctt cattataaga
    79321 atttatgcac tatgtaggcc ggataaggcg tccacgccgc atccggcaca ggcaccgtgc
    79381 tgatgtctga tgcgacgctg gcgcgtctta tcagacctac aacccccccc ggcgaatgta
    79441 cgcagctaca ttaattccgc cgttcgaatg ccagtgcttt gcgctcgatc agacgcatca
    79501 tcagcgtcag caggccgttg acgaccaggt aaataatccc tgccgcaccg aacaccatta
    79561 catcgtaggt gcgtccgtac aacaactggc tgtatcccat cacttccatc agcgtaatgg
    79621 tgtaagccag agaggtactt ttgaacacca gcaccacttc gttggaataa gaagagagcg
    79681 agcgtttaaa ggcatacggc agcaggatcg ccagcgtatc ttttttgctc attcccaggg
    79741 cgctacagga ctgccactga ccttctggga tcgcacgaat tgcaccgtaa aacagctgcg
    79801 tggtatacgc cgcactgttc agcgataacg caatcagcgc acataaccac ggttctgaca
    79861 acaaatgcca cagtgccgga tactcctgca aagtcggaaa ctggcccggc ccgtaataaa
    79921 tcaggaagat ctgcaccagc agcggcgtac cggtaaacag cgtgatataa ccccgcacca
    79981 gccacaccag caccggcgtt ttcagcgtca ggatgatggt aaaaatcaat gccagaatca
    80041 gtgccacaat cagcgaggca acggttagcg tcaggctggt gtgtagccct ttcatcagtt
    80101 cgggtaaata ctcaaacatt agctgggcct ccgctcaaaa cgtgtcgcgc gcaggtcaat
    80161 gcgtttgaga atgtactgac tgagcagggt gatcaccagg taaatcgccg ccgccacaat
    80221 gtaccaggta aatggttcct gggtacgagt agcgatgctt tttgtttgca gcattaaatc
    80281 attcacacta atcaaactga ccagcgcggt atctttcagc agcaccagcc actggttacc
    80341 gaggccaggc agcgcatgac gccacatctg cggcatcacc agacgtaaaa agatagccga
    80401 tttcgacagt cccagcgcct gaccagactc ccactgaccc accggcaccg ctttcaacgc
    80461 gccccgaagc gtttgcgagg cataggcggc atacagcagt gacagagcga tgacaccaca
    80521 aaggaacggg ctaacgtcga agttctcaat gtccatctgc actgggatct gcacgaaccc
    80581 aagattgata gtgaagccat ccgaaagcgt cagcagcagc tgcgaggagc caaaatagat
    80641 aaacagcacc accagaattt ctggcaggcc acgcagaatg gttaccagcg ctgaacctgc
    80701 ccacgcgaca ggacgccatt ttgccgactc ccataccgca aagaacatcg ccagcgccag
    80761 cccgacaatc aatgcacaaa cggcaaggcc gacggtcatc ccggcggcgc ttgctaaagg
    80821 aaaaaattca ttcatcagga attacttctg gaaccatttg ttgtagatgg tttcgtaagt
    80881 gccatctttc ttcacttttt ccagcgcagt gttgagtttc tgctgcagct cagtgttgtc
    80941 ctgacgtacc gcgatgccga ggccagtgcc gaagtaatct ttatcggtca ctttgtcgcc
    81001 caccgccgcc agtttcgggt tatctttcag ccactcagtg accactgcgg tgtcaccgaa
    81061 gacgccgtcg atacgcccgt tttgcagatc cagttttgcg ttctggtagc tgtcatacgg
    81121 aacggtagtg atttccgggt gcttatccat aatgaatttc tggtgtgtcg tcccgttctg
    81181 tacgccgact tttttgcctt tcagctgatc aacactggtg tatttgcctt gctgacccac
    81241 aaacagggca gagttgtcat agtacggggt ggtaaacagc acctgctttt cacgctccgg
    81301 agtgatatcc atgcccgcca tcacggcttc tacgcgacgg aatttcaggc ttgggatcag
    81361 gctgtcaaac gcctggttag agaaagtgca ggttgcatca atctctttac acagcgcttg
    81421 tgccaggtcg acgtcaaaac caacgatctg gttgtttgca tcaatcgatt caaacggagg
    81481 ataggatgct tcggtagcaa aacgaatggt ttcggcagct gtggcggaaa gactaaaacc
    81541 tgcaattaac gcggcaatca gaactttttt cattgttgtt atcccgaatc ttagtgagag
    81601 agatagtttt taaatgcttc ggtttgcggc tcggtaaagc agctcgcgtc gccttgttct
    81661 acgatatgac cattttccat atacaccact cggctggcgg ttttacgcgc cacttcaact
    81721 tcgtgggtga cgatcacctg ggtaatattc gtttctgcca gctcacgaat gatgctgacg
    81781 atttgtgccg taatttccgg gtccagtgcg gcggtcggtt catcgaacag cagtacctgc
    81841 ggttccatca tcaacgcacg ggcaatagca acacgctgct gctgaccacc agaaagatgc
    81901 agcgggtaac gatcgctata aggtttgaga cgcagacgtt ccagcagttt ttctgcacgg
    81961 gccagcgcct gatctttact caaccccagt acacggcagg gcgcttcaat caggttttgc
    82021 tgcacggtca gatgcggcca caggttgtat tgctgaaaca ccatgccaac gttacgacgc
    82081 aaatcgcgaa tcgctttgtc agagggtgtt ttggtgaaat cgaaatggtt gcctgcaatg
    82141 ttgagcgtac cggagcgtgg catctcaagc agattgagta cacgcagcag cgagctttta
    82201 cccgcgccgc tggggccaag taacaccagc gtttcgccct gtgggcaatc cagcgtgata
    82261 tcgaacagcg cctgatgcgc gccgtagaag caattaatgc cgtttaattg aatactcatt
    82321 gacactcgta tactggcagt ctgatagcta ttgaggtcga agatagtacc tttgacagaa
    82381 taattatgca atatttctgc tttaaaagtt aaaagcaaag cgcattattc aataaacata
    82441 gcacaaaata acgggggcgg tggtcggcga gcataaatgt cggcattcct cacgaaatgc
    82501 cggacaattt acggggttta ttggttgatc aaggcgttag cgattctcga tggactgacg
    82561 gagcgtaccc gccgtggcat gaacgctgcc gcctaagtag cgtacatcgt cgatcaccca
    82621 acactggcct tcctgaatca ttaacacttc atcctgccag ctctgatcac cctgttttag
    82681 atccacacgc agaggaatat ttcgcgcatc acgattcggg attgttgagg cactggcaac
    82741 acgggcgctc tctggcaagg ttgtgcgact ggagaatgga tcgttggtca gtagttcccg
    82801 atggttatta tcccgggagg catcgctaag cagtgtcgcc agtttgtcgc tcagataagg
    82861 gcgcaaggcg gtgatgtcgt tgctgcggtg caaaatgcgg tagtcataaa attgctgggc
    82921 cacgttatcc gggcctcctt caacgcaagg accactgcgt gtgccgttat ctttataagc
    82981 tggagtgact gtggtgcagg cactgaggag cagtgcgcag gggataagca ttgtcaattt
    83041 gctgtagcgc ataatgattt ccttataagc gatcgctctg aaagcgttct acgataataa
    83101 tgatatcctt tcaataatag cgtatcagtc tgataatgct tttgagatcg aaggcttagc
    83161 aaacaaggag atcgatcatg caattttcta caaccccaac tctggaaggc cagaccatcg
    83221 ttgaatattg cggtgtggtg accggcgaag cgattttagg tgccaatatt ttccgtgatt
    83281 tctttgccgg tatccgcgat atcgttggcg gacgttccgg tgcgtatgaa aaagaactgc
    83341 gtaaagcacg ggagatcgcc tttgaggaat taggctccca ggcgcggacg ctgggggccg
    83401 atgccgtcgt cggtattgat atcgactacg aaacggtcgg gcaaaacggc agtatgctga
    83461 tggttagcgt cagcggtacg gcggtgaaaa cgcgtcgatg agaagagtct tctggctggt
    83521 cgctgccgct ctgttattgg cagggtgtgc aggcgaaaaa ggcattgtcg agaaagaggg
    83581 atatcagctt gatacccgac gccaggcgca ggcggcgtat ccgcgcatta aagtgctggt
    83641 gatccactac accgcagatg attttgatag ctcgctggcg acactgaccg ataagcaggt
    83701 cagctcgcat tatctggtcc ctgcggtacc accgcgatac aacggtaaac cgcgcatctg
    83761 gcaactggtg ccggaacaag aactggcctg gcatgcgggg attagcgcct ggcgcggggc
    83821 aacgcgcctt aacgacacct ctattggcat tgagctggaa aaccgtggct ggcaaaaatc
    83881 ggccggagtg aaatattttg ccccgtttga accggcacag attcaggcgc ttattccact
    83941 ggcgaaagat attattgccc gttatcacat caagccggaa aacgtagtgg cacatgcgga
    84001 tatcgcaccg cagcgcaaag acgatccggg gccattattt ccctggcagc aactggcgca
    84061 gcaggggatt ggtgcctggc cggatgcgca gcgggttaac ttttaccttg ccgggcgcgc
    84121 gccgcacaca ccggtagata ccgcgtcatt gctggagctt ttggcgcgct acggttatga
    84181 cgttaaacct gatatgacac cgcgcgagca gcggcgcgtg attatggcat tccagatgca
    84241 tttccgcccg acgttatata acggcgaagc ggatgcagaa actcaggcga ttgccgaagc
    84301 attgctggag aaatacgggc aggattagcg cggcagtttt ccgtggtcgc gtagccaggc
    84361 ggcagttttc tcgatacctt catccagggt gatgaccggc tgataaccta actcttcctg
    84421 cgcacgcgta atatccagcg taaagtcaaa attcaacttg gagacgccgt agtgggtcag
    84481 cggcggctct tttgctgact tgcggcctaa acgctccatg ctgcgggcga tcatatccag
    84541 catcgggtag gggacggaac gaatacgaca gtcaatattc aactcgtcga tcagcttctg
    84601 cacgatgctg cgcagtgtgc gatgctcgcc gttggtgatg ttgtacacac gcccggaagg
    84661 tagcttatcg caggcttcct ggcttgccag ccacattgcg tgcacggcat tttcatagta
    84721 ggtcatatcg accagcgcac tgccgccatg cggtaacaga atactgccgt agtggtgcat
    84781 catatgcgcc agacggggaa taaagacttt atcgtgcggt ccgaacagac tttgtgggcg
    84841 cagaatagta aagcgcgttt gtggattcgc ctgcgaaagc atattgatca cttcttcgct
    84901 ggctgctttg ctgcgggcaa actcgttggc gaagcggtga gggcgaaaat cttctttaat
    84961 atcgcgatgg tggtgataat cgaagtacag ggagggggaa gagatatgaa taaagttacg
    85021 cacaccccag gcgacagccc attcacccag gcggcgagtg gcgcgaacgt tagccagatc
    85081 gaaagcctgt tgtgtccccc agggtgaggt aaagctggag cagtgccaca gcgtatcaat
    85141 gcccgcgagc atcactttag cttgtgatga aaccagctcg gtcagatccg ccggaacaaa
    85201 ctctgcgccc attttttcca gcaatttgcc cattgcctcg ttgcgaccgg tcgctcgcac
    85261 gctgatgcct ttctggcata aaaactctac cgcgtttcga cctaagccgc tggtggcgcc
    85321 ggtaaccagt accttcatat caatccactg ttgttgagaa aataacgtgc gcattcttcc
    85381 gtgatttccc ccatgatgca atgggaaaca tgaaagaata acgcaggttt tgtcgattaa
    85441 tctgtgcttt gttctgccag tctggcgatt tgttttgcca ttccgcgaaa aataaacaga
    85501 tgcgcgggga tcatcaataa ccagtaaaac agccccggca taccgtgcgg atgccagaaa
    85561 gcgcggacat cgatagtacg atagtcgcct ttatcttcca ggctaaaaca cagtcgtccc
    85621 agccccggcg ctttcatgcc aaataacaac gtaagttgtt tttccggttc aacgacaatc
    85681 actttccagc tatctaccgc atcgccagtc tgtaaatatt cgcgctccgg gcggcctttc
    85741 gccagcttat gaccgatcgc gcggtccatc aacgcccgtg tctgccacaa aatattgcca
    85801 aagaaataac gctctttacc gccgatttgg ttcactacct gccataaagc agcaaggctg
    85861 gcggacgttt taacggtaaa ccccgcctgt ttggcaaaat aaccgtactc cggtcgccag
    85921 cgggcaaagg cctgagcgtc gtggccccag tcgctggagt tgaccagttt ttcctcctct
    85981 ttcaacgtgc tacgtaccgc gtcatcgaaa gcgatcagcc gttgtgggat gagtgcacgt
    86041 agcgcggtat catccgccag cagatcgtgt ttcagcccct gaatcaacgc cctggcggtg
    86101 gtgggcggta cggaagtaat cacattgaga aaccacaccg aaatccagcg ggtggggagg
    86161 gggatgggga tcaaccagcg gcgcttaccg ctcaccgcca taaaatgttc aaactgttgc
    86221 tgataactga gcacctctgg tccggcggct tcgaagatgc ggtgttcgct ggccggatga
    86281 tctaacaacg ccaccagata gtgcagcaag ttttccagcg cgatgggcgt ggtgcgtgaa
    86341 cgtacccagc gtggcggcgt taacactggc aggttgtaga ccatatcgcg catgacttcg
    86401 aacgccgctg aacctgcgcc aacgataatt ccggcccgaa gttcggtcac aggtacattc
    86461 gcttcacgaa gaatgtccgc cgtagcctga cgagcacgca gatgatccga ctgctcatgt
    86521 ggcggggcct gcaacgaact gagaaagatt aattgcttaa ctggtacttc acgtagcgca
    86581 tcgcggacgt tgagagccac ctggcgctcc tgagcgataa aatcgccgcc ttcgcccatg
    86641 ctgtgcacca gaaaatatac ggtatcgata tcctgcaaca gggccggaag gttatccggc
    86701 cagctgagat cgactttatg gcaactgacg tttgccagtt gcagctttgc aagcctgtcg
    86761 acatgacgtg ccgccgccag gatctgatgc ccttgctggc tgagtgtgcg caccagatgc
    86821 tgaccaatgt agccactggc accgagaact aaaatgcgtt gcggcacgtc tctctcctta
    86881 acgcgccagg aatgcgcgcc agtgggcggc gacttccgcc agttgttcgc gcgagacgtc
    86941 aagatgcgtc accaggcgga caatcggcga ggcgttaatc agcacgtttc tcgctttcat
    87001 gtattcgcct aacgcggcag cattttcttc cccgacgcga acaaacagca tattagtgtc
    87061 ctgacgcatc acatccgtgc ctgcttcacg cagttgctcc gccatccagg cggcgttgtc
    87121 gtgatcttcc tgcaaccgtg cgacgttatt tttcagggca tacatcccgg cggcagccag
    87181 aatcccggac tgacgcatcc cgccaccggc cattttccgc cagcggatgg cacgtttaat
    87241 gtaatcacga ttaccgacga gtaaggaacc gactggcgtc ccaagacctt tcgacaggca
    87301 aatggtgaac gaatcacaat attgcgtgat ctctttcagt tcgcagccgt aagccaccac
    87361 ggcattaaag atgcgcgcac cgtcaacatg cagcgccaga ttgcgctcgc gggtaaattc
    87421 ccatgcttct ttcaggtatt cacgcggcag cactttgccg ttgtgggtgt tttccagact
    87481 gagtaatttg gtgcgggcga aatggatatc gtcgggtttg attttcatcg ccactttatc
    87541 cagcggtagc gtgccgtcgg cagccgcgtc gatgggttgc ggctgaatac tgcccagcac
    87601 cgctgcgcca ccggcttcaa acagatagtt atgcgcggcc tgaccgacaa tatactcttc
    87661 gccacgttcg cagtgactga gcagagcgac caggttggcc tgagtgccgg taggcagaaa
    87721 aatggcggct tctttaccgg aaagctctgc tgcatagtcc tgcagagcat taacggtagg
    87781 gtcgtctccg taaacgtcgt ccccaaccgg ggcggccatc atcgcttcga gcatggcgcg
    87841 gctcggtcgg gtaacggtat cactgcgtaa atcaatcatg gcatgtcctt attatgacgg
    87901 gaaatgccac cctttttacc ttagccagtt cgttttcgcc agttcgatca cttcatcgcc
    87961 gcgtccgctg atgattgcgc gcaacatata caagctaaat cctttggcct gttcgagttt
    88021 gatctgcggt ggaatggcta actcttcttt ggcgaccacc acatccacca gcaccggacc
    88081 gtcgatggag aaggcgcgtt gcagggcttc atcaacttca gacgcttttt ctacacggat
    88141 acccgtaatg ccgcacgctt cggcaatgcg ggcaaagttt gtgtcgtgta gttcggtgcc
    88201 gtcagtcaaa tagccaccag ctttcatctc catcgccaca aagcccagca cgctgttgtt
    88261 aaagacgaca attttcactg gcagtttcat ctgcactact gagaggaaat cgcccatcaa
    88321 catgctaaaa ccgccatcgc cgcacatggc gaccacctga cgttctggct ctgtcgcctg
    88381 cgcacccagc gcctgcggca tggcgttagc catcgaaccg tggttaaacg aacctaacag
    88441 gcgacgcttg ccgttcattt ttagataacg tgccgcccac accgttggcg taccaacgtc
    88501 acaggtgaaa atagcgtcat cggcggcaaa atgactaatt tgctgcgcca gatattgcgg
    88561 gtgaatggct ttctcgctcg gtttagctaa atcgtccagc cctttgcggg cgtcgcggta
    88621 atcttccagc gctttatcca gaaacttgcg atcggctttt tcttccacca atggaagcaa
    88681 tgcacgcaga gtcgacttga tatcgccgac cagtgccata tccaccttgc tgtgagcgcc
    88741 gatactggct gggttgatat caatctgaat gattttggca tcggtcgggt agaaggcgcg
    88801 gtagggaaat tgcgtgccga gtagcactaa cgtgtcggcg ttcatcatgg tatggaaacc
    88861 tgacgagaag ccgattaacc cggtcattcc aacatcatac ggattatcgt attcgacatg
    88921 ttctttaccg cgcagggcat gaacaatagg cgctttaatt ttcccggcaa actcaactaa
    88981 ctctttatgc gcccccgcgc agccgctgcc acacatcagg gcgatattgc tggaataacg
    89041 cagcagttgc gccagtttgc gtaactcttc ttcttccggc gtcacgactg gttgtggcgc
    89101 atgataccag tgcgtggttg ccccttctgg cgcaggtttt aacgccacgt cgcctggtaa
    89161 cacgacaacc gaaacgccac ggttaagcac cgctttgcgc atggcaatcg ccagtacttg
    89221 tgggatctgc tccgggctgg aaaccagctc gcaatagtga ctacattcgc ggaatagctc
    89281 ttgtgggtgg gtttcctgga aatagccgct gccaatttcg ctggagggaa tatgagcggc
    89341 aatcgccagt accggaacgt gattgcggtg gcaatcgaac aggccgttga ttaagtgcag
    89401 gttgccgggg ccgcacgatc cggcgcagac cgccagttct ccgctaagtt gtgcttcagc
    89461 gccagcggca aaggccgcca cttcttcgtg gcgggtggac atccactcga tggtgcccat
    89521 gcgattaaga ctgtcactaa gaccgttcag agagtcgcct gtgactcccc agatgcgttt
    89581 cacccctgcc gattcgagtg ttttggcgat ataagctgca accgtttgtt tcatggttct
    89641 ccatctcctg aatgtgataa cggtaacaag tttagttcat ctgacggagg gggaagggat
    89701 gggagagaaa ggaggcacta acggttaaat agcccgatga aaggaatatc atcgggcata
    89761 aggcgattat gcgagaacca aatccccctg cggatggcag gagcaggcca gtacgtaacc
    89821 ttcagcgatt tcggcgtcgg tcagcgtcat tgtgctgctc accgtatatt caccggaaac
    89881 cacttttgtc ttacagcagc cgcaaacacc cgcacggcag gcggcgacaa ccggaacgtt
    89941 attgctttcc agcgcctcca gtagcgtggt gccaaccggg gcgtaaaatt ctcgtgccgg
    90001 ttgcagtttg gtgaatttca gaccgctggt cgctgcttcc gctactgggg tgaagaattt
    90061 ctctttaaag aaacgcgtca cgccgagcgc tttcacttcc tgctctaccc aatccatata
    90121 cggagccggg ccgcaggtca tcacggtacg tgaagctaag tcaggaacgc gagtcagcag
    90181 ttcgcgagtg agacgaccag cgataaagcc ttcggtaacg ttattttccg ccaccagcgt
    90241 taccggatag ttacgccact catcggcgaa aataacatcc tgcggcgtgc gcacgttgta
    90301 gatcacctgt acatcggcct gtggacagtt cttcgccagc cagcgacgca tcgacataat
    90361 cggcgtgacg ccgcagcctg ccgccagcaa caggaattta tcttctgctt tatcgtcgca
    90421 ggtaaattcc cccatcgcgt ccgaaagcca gagataatca ccgcgtttta catcgcgcgt
    90481 cagccactgg gagccgacac cgtcatcaat ccgccgcacg gtcagggtga tatattcact
    90541 cacgcctggc gtggaggaaa tggtgtaagc acgcagcgtt tccgctgagt ttcgcacgct
    90601 gaccagtgca tattgcccgg cgcgatatgg gtagtaatcg tggcaaatca gggaaatcgt
    90661 ccacacatcc ggcgtttctt gcgtaatgtg atgaacctgc atccgccacg ggcattgatt
    90721 cgttggcatc gtcatcgaca aactccttac gcgctcaaca gttgcttcat gtcttcttca
    90781 acagtggtga tagaacgcag gccaaatttc tcgttcagca ccgccagcag gtctggtgtc
    90841 aggaaaccag gtgcagtcgg gccggtgacg atatttttca cgcccagaga aagcagcgtc
    90901 agcagaatga cgatcgcttt ctgttcaaac caggagagca ccagcgacag cggcagatcg
    90961 ttgacaccgc agcccagttt ctctgccaga gtgacagcca gaataatcgc tgagtaagca
    91021 tcgttacatt gacctgcatc taccagacgc ggcagacctt cgatatcgcc aaactcaagt
    91081 ttgttaaagc gatatttacc acaggcgagg gtcaggatca ggcagtcatc cggcacgctg
    91141 gtggcgaaat cggtgaagta gtggcgctcg ccgcgtgcgc cgtcacagcc accaagcagg
    91201 aagatatgac gcagtttttc acggctcacc agatcaatca gcgtatccgc tgcgccaagc
    91261 agcgtctggc ggccaaagcc cacggtgatc aggtgcggaa tttcgctgta cgggaagccc
    91321 gccatttgtt gcgcctgggc gatgaccgca gaaaaatctt cgccatccag atgacgcaca
    91381 cccggccagc caacgatgct gcgggtccag atacgatcgt cataagcgcc tacggttggg
    91441 tcgatgatgc agttcgaggt catcacgatg gggccaggga aacgagcgaa ctccacttgc
    91501 tgattctgcc agccgctgcc gtagttaccg accagatgct tgaatttacg cagctccggg
    91561 tagccatgcg caggcagcat ttcgccgtgg gtgtaaacat taacgcccgt gccttcggtc
    91621 tgttccagca ggttgtagag atctttgaga tcgtgaccgg aaatcaggat gcatttaccc
    91681 gccgtcgctt tgacgttgac ctgggttggc gtcgggtgac cgtatttacc ggtttcgcct
    91741 gcatccagaa tgctcatcac tttgaagttc atctggccga tttccattga acactcaaga
    91801 agcgcgttca tatcggcagg ccaggtcccc agccacgcca tgattttatg atactgggca
    91861 taaatatcgt tgtcgtattg accgagaaca tgcgcgtgtt ccatataggc cgccgcacct
    91921 ttcaggccat acaggcacag cagacgcagg ccgagaatgt tttcgccaat cgccgcttta
    91981 tctttgttag gggtaaattc tgctgcctga cgttgcagct cgccgagatc gtcgctcacc
    92041 agttgcaggt cagccatcgg gttatcgacg cgcgcgttgg catctacagc caggcattgc
    92101 gctttcagcg cctcgcgcag ggcaatcgct tcacgagcgt agccgacaat acgcggagaa
    92161 tcgaagttaa cgttggtcag ggttgagaaa aaggcacgtg gcgcgaagct gtcaacatcg
    92221 tggttgatga tgccgtattc acgcgctttt accgcccagg cagaaagccc ttgcagcgcc
    92281 gcgatgagta aatcctgaag gtcagaagtt tccgccgttt taccacacat cccctgcgcg
    92341 tatgagcagc cgtttcctgc cggagtacgg atagtttgtt cacattgcac acaaaacatg
    92401 atcacacctt ttaaagttat atttaatata catgtttaag gttaagacgc ttaacgcggg
    92461 gataaaaggg atttttcatg caactttaaa ggagattgat ttagcgcaat tttggcggca
    92521 gggatctacc gccagagagg tattacgcag agaaaaaggc gatgaggatc ggcactaaca
    92581 ggctaagaat aaaaccgtga acaattgccg ccgggaccat atccagcccg ccagtacgtt
    92641 gaagaacggg cagggtgaaa tccattgatg tggcaccgca taagcccagt gcagtagagc
    92701 ggctgcggcg aatcagccca gggatcaaca taatagcaat cagttcacgg gccagatcat
    92761 taaaaaacgc cgcgctcccg attaccggac caaaagattc ggtcaataaa ataccggaaa
    92821 gagaatacca gccgaaaccg gaggccattg ccagcgcggt attgatgggg agatcaagaa
    92881 taaaggcgtt aattaaacca ccaattaatg aactgacaac caccaccacg gcgacaatca
    92941 ttccccggcg attaaggaca atctgcttta aggtcatgcc attattgcgc aactgaatac
    93001 caacgaggaa aagtagcaaa attaacgtgt attcactggc ttcggtcgcg tgttgtaaga
    93061 aagccagtcc actcagacca atggcaaaac caatcactac tacgccgcac agttttagcg
    93121 actccagcgc catcgcaata cgcgacggga gtttttcttg ctgatggtgg ttgcgccacg
    93181 gcaggcctcg ctccagccac atcagggcgg caatattaca cagtaaaata acggtaatac
    93241 tgacggcaga ataatgcaga atcgccaaca ggttactggc gaggttatcg agaaacgcca
    93301 gactgatacc cataaaaaag agaataaggt aaaccatcca gcttaatagc tgattaataa
    93361 cttttaacgt agcttgttgg cgaagcggaa tgaggtaacc cacaatcagg ggaaccagaa
    93421 tgattaacag cccagaaaac atgaaaaccc agtccttgca aagatgaagt cgaaatgcgc
    93481 gatgacacac tactgaaagc ggaaggacga gtaaagttgc aattaaaagg aaatgttatg
    93541 cataaggagc agtagagtat tcgttttcat ttaaagatat tcttgcgctt taattacaaa
    93601 ctgcaccgat gttggtggcg tcaaaatcgc cgaggcgttc cctgaaggcc ggggcagccc
    93661 acatggatgt gggctgaggg cgcgttttac agggatgtta cctcgcgccc gacccggtag
    93721 ccgtaaggga taagtcgagg gcaccgcgca gcggcgattt tgttcgccag agcccggggg
    93781 tgcatggggc ggcggcgatt ggccgccccc tgcgcgctcc ttgcgccagt ggcaatatgt
    93841 ggcttagctc atgaaaggag cgcaacaaga tgatgaatca acatataaca acatcttaaa
    93901 aaaaggcctg acattacgcc aggccttctg cgttaattaa tcacgctttt ccagcagggt
    93961 ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga agaaccacag
    94021 ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg ccgggttaac
    94081 agaagtgtta gtcaccggaa tactaattaa gtgaatcagg gttaaggcca gaccaatagc
    94141 gatcggcgca aaacctgccg gcgcgaattt gtcggttgcg ccgtggatca ccaacaggaa
    94201 acctgcactc aataccagtt caactaccag cgcggaaagc atggaataac cgcctggtga
    94261 atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcatcaaaac ccgttttacc
    94321 actggcaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct gggcaattac
    94381 gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa tagtgaccgc
    94441 cgggttaaaa tgaccaccag aaatatgacc aacagcaaag gccatcgtca gaacggtcag
    94501 accgaacgcc aacgccacgc cggcaaaacc aatgcctaat tccgggaagc ctgcggccag
    94561 tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt cagctgctaa
    94621 ttttctgaac atatccacct caattaaaaa ttgaccctgt gaaaaatatg gtcgttttat
    94681 agggtcgtcg taaaaaagtg acgacggaaa taatgcgcgg ctattttaaa aacgaaggcg
    94741 agtcattcac cagataaata aatccagtaa atttgattta gggcaacagc gggttgcccc
    94801 atatagtcat ttgtctgatt gacagtgtag tgcaagcaaa agatttaatc ctttaggcgt
    94861 aataaaaaat aatttatcat gctaattatt tgattttgtt gtttttgcag acttatcagc
    94921 aagagggagt ataacgcgat tattcgctca tttttcagac atttgccatg cttaaatgtg
    94981 atgtcatcac gtattagcaa ggcctttccc gttatactgc cagcgtaaag gataagtcac
    95041 atatttctgg aggggatatg attcttgagc gcgttgaaat tgtgggtttt cgcggtatca
    95101 accgtttgtc gttgatgctg gaacaaaaca acgtcctgat tggggagaac gcgtggggta
    95161 aatccagctt gctggacgcc ttaaccctgc tgctatcgcc agaatcagat ctctaccatt
    95221 ttgagcgcga cgatttctgg ttcccgccgg gagatatcaa cgggcgagaa catcatctgc
    95281 atattatttt gaccttccgc gaatcgctgc caggccgaca tcgggttcgc cgttatcggc
    95341 cgctggaagc gtgctggacg ccatgcaccg atggctatca ccgtattttt tatcgtctgg
    95401 aaggggagag tgcggaagac ggcagcgtga tgacactgcg cagttttctc gataaagacg
    95461 gacatccgat tgatgtcgag gatattaacg atcaggcacg ccatctggtg cgtttaatgc
    95521 cggtgctgcg cttgcgtgat gcccgtttta tgcgccgtat tcgtaacggc acggtgccaa
    95581 atgtccctaa tgtggaagtc accgcgcgcc agctcgattt cctcgcccgt gagttatcct
    95641 cacatccgca aaatctctct gatgggcaga ttcgtcaggg actttccgca atggtacagc
    95701 tgcttgagca ttatttctct gagcaggggg ccggacaggc gcgatatcgt ttaatgcggc
    95761 ggcgagccag caatgagcaa cgaagctggc gctatctgga tatcatcaac cggatgattg
    95821 accgacctgg tgggcgctcg tatcgggtta ttttgctcgg cctgtttgct actttgttgc
    95881 aggcaaaagg cacattgcga ctggataaag acgcccgtcc attgttgctg atcgaagatc
    95941 cagagacccg tttacacccc attatgcttt cagttgcctg gcatctgttg aatcttctgc
    96001 cattgcagcg cattgccacc accaactcgg gtgagttgct ttcgttaacg ccggtagagc
    96061 atgtttgccg actggtacgt gagtcctcgc gcgttgccgc ctggcgtctg gggccgagtg
    96121 gcttgagtac cgaagatagc cgacgcatat cctttcacat tcgttttaac cgtccgtcat
    96181 cgctgtttgc acgctgctgg ttgctggtgg aaggggaaac ggaaacctgg gttatcaatg
    96241 aactggcgcg tcagtgcgga catcattttg atgccgaagg gatcaaggtc attgagtttg
    96301 cccagtccgg gctaaagcca ctggttaaat ttgcccgccg aatggggatt gaatggcatg
    96361 tactggtcga tggcgatgaa gcagggaaga aatatgccgc tacggtacgc agcctgttga
    96421 ataacgatcg ggaagccgaa cgagaacatt taacggcgtt accggcgctg gatatggaac
    96481 attttatgta tcgccaggga ttttccgatg tgttccaccg cgtggcgcaa atcccggaaa
    96541 atgtaccgat gaatctacgc aaaattatct cgaaagcgat ccatcgctct tccaaacccg
    96601 atcttgccat tgaagtggca atggaggcag gacgtcgtgg tgtggactcc gtaccgacgc
    96661 tgctgaaaaa aatgttctca cgcgtgctgt ggctggcgcg cggtcgcgcg gattaaccgc
    96721 gaaacatcgt ggccatttgt ggctgaatag cgtcgagcat ctcatagcgc cgacggtatt
    96781 cagcccgttt tttactggcg atttcggcaa tctcttttcg tgctatctgt gctggaaggc
    96841 ggtaatggcg ttcagcatca catacgccgc caaccgattc ccagaaagcg ttgtaatcag
    96901 cgtggatctt gccttcttta tcgcgataac gcaggctgcg gtaaatatgc gtttcattgc
    96961 tgacggcaat aatctgctct acctgcaaac gttgggcaaa cagacaggcc gcttccatca
    97021 cgaggcgttt gggaaatagc ccgtggcagg ctttcgtcgc attctggatt tcctgatgtg
    97081 gaatttccca ttttgcgcct tgcagtccgc caataaacat cgttcttttc ccctgatatt
    97141 cacacagggt aaacgtgatc tctgccagag gaataccttc gctgttgcga aacaggattg
    97201 tgctgtcacc ttctttatcc attgagatca tcatggtcag ctcaagcgtg aactgctcgc
    97261 cgtttttgcc ttccagcttc gccagttgca gcccgggggt attcaaatat aagctgaatt
    97321 cttccgccga catacatccg cggagtaacg cataatggta acgtaacgcc tccagcaatt
    97381 gcttacggct aagattcgcc gcaaggtaag ggcgatgcag acgcacaggc agtcgcggtt
    97441 ggcgcgttaa caacacattg agattaggcc agtgggaaag ttcgttcatc cactcaacgc
    97501 ttaaacgcgg cataatcaac gagcgcagca aaaatttctg gcgaaaacta cggcgatgcc
    97561 agaatttacc cggccgacac tgtccacgtg ccagactaag aaaaagtgac aggctgctga
    97621 gagattcaga tggcgtaaag gtccgttcag ttagctgcga catattcatg aaatcaatgg
    97681 ttatacatga cgtcgatttc accattgcgt atcttaacca aacatcaata gtgtgattac
    97741 taacgtaaat tttatggttt tgttgatatt tcgttgaagt taatgacccg gattggcata
    97801 tggagtattc agaatattta tgaaaaagcg gaaaaccgtg aagaagcgtt acgttattgc
    97861 gctggtgata gtcatcgccg gactgattac gttatggaga attcttaacg cacccgtgcc
    97921 gacttatcag acactgattg tgcgccccgg tgatttacag caaagcgtgc tggcgaccgg
    97981 aaagctggac gcgctgcgta aggttgacgt gggcgcgcag gtcagcggtc agttgaaaac
    98041 gctgtcggtg gcgattggcg ataaagtaaa aaaagaccag cttttagggg tcattgatcc
    98101 tgaacaggct gaaaaccaga tcaaggaggt cgaagcaacg ctgatggagc tacgcgcgca
    98161 gcggcagcag gcggaagcgg agctgaaact ggcgcgggtg acgtattccc gtcagcaacg
    98221 tctggcacaa acgcaggctg tttcacagca ggatctcgac accgccgcga cggagatggc
    98281 tgtgaaacag gcgcaaattg gcaccattga cgcgcaaatc aagcgcaatc aggcttctct
    98341 cgatacggct aaaaccaatc tcgattacac tcgcatcgtt gccccgatgg ccggggaagt
    98401 cacgcaaatc accactctgc aaggccagac ggtgattgcc gcacaacaag caccgaacat
    98461 tctgacgctg gcagatatga gcaccatgct ggtaaaagcg caggtttctg aagcggatgt
    98521 aatccacctg aagccggggc aaaaagcctg gtttacggta cttggcgatc cactgacgcg
    98581 ctacgagggg caaatcaagg atgtactacc gacgccggaa aaggttaacg acgctatttt
    98641 ctattacgcc cgttttgaag tccccaatcc caatggtttg ctgcggctgg atatgactgc
    98701 gcaagtgcat attcagctca ccgatgtgaa aaatgtgctg acgatccctc tgtcggcgtt
    98761 aggcgatccg gttggcgata atcgttataa agtcaaattg ttgcgtaatg gtgaaacacg
    98821 cgagcgtgaa gtgacgattg gcgcacgtaa cgataccgat gttgagattg tcaaagggct
    98881 ggaagcgggc gatgaagtgg tgattggtga ggccaaacca ggagctgcac aatgacgcct
    98941 ttgctcgaat taaaggatat tcgtcgcagc tatcctgccg gtgatgagca ggttgaggtg
    99001 ctgaagggca tcaccctcga tatttatgcc ggtgagatgg tagcgattgt tggcgcttca
    99061 ggttccggta aatcgaccct gatgaatatt ctcggctgtc tggataaggc caccagcggc
    99121 acctatcgcg tcgccggtca ggatgttgcc acactggacg ccgatgcgct ggcgcaactg
    99181 cgccgcgagc atttcggctt tattttccag cgttaccatt tactttcgca tttaaccgcc
    99241 gagcagaacg ttgaagtacc cgccgtctat gccggtcttg agcggaaaca gcggctgctt
    99301 cgcgcccagg agttactgca acgactgggg ctggaagacc gtacagagta ttatccggca
    99361 cagctttcgg gtggtcagca acagcgcgtc agcatcgcgc gggcattgat gaacggcggt
    99421 caggtaattc ttgccgatga accaaccggc gcactggaca gccattctgg cgaagaggtg
    99481 atggcgatcc tgcatcagct gcgcgatcgc gggcatacgg tgattatcgt cacccacgat
    99541 ccgcaggtcg ctgctcaggc cgagcgggtg atcgaaattc gcgacggcga aattgtgcgc
    99601 aatcctcccg ccattgaaaa agtgaatgtt gctggcggga cggagcctgt tgtcaacacg
    99661 gtgtctggct ggcggcagtt tgtcagcggt tttaacgagg cgctgacgat ggcatggcgg
    99721 gcgctggcag cgaataaaat gcgtacttta ctgaccatgc tggggattat tatcggtatt
    99781 gcgtcggtgg tttccattgt cgtggtgggt gacgccgcca aacaaatggt gctggcggat
    99841 attcgttcta ttggtacgaa tactattgat gtctatcccg ggaaagattt tggcgatgac
    99901 gatccgcaat atcagcaggc gctgaagtac gacgacttaa tcgccatcca aaaacaaccg
    99961 tgggtcgcct cagccacacc tgccgtctcg caaaacctgc gcctgcgtta taacaatgtt
   100021 gatgttgctg ccagtgccaa tggcgtgagc ggcgattatt ttaatgtcta tggcatgacc
   100081 ttcagtgaag gaaacacctt taatcaggag cagctgaacg gtcgtgcgca ggtcgtggtt
   100141 ctcgacagta atactcgccg ccagcttttc ccccataaag cagatgtggt tggcgaggtg
   100201 attctggtcg gcaatatgcc cgccagagtc attggtgtgg cggaagaaaa acagtcgatg
   100261 tttggtagca gtaaagtgct gcgtgtctgg ctaccttaca gcacgatgtc cgggcgagtt
   100321 atgggccagt cgtggcttaa ctccattact gtcagggtga aagaaggatt tgacagcgcc
   100381 gaggcggaac agcaactcac gcgtttactt tcactgcgcc acggtaagaa ggatttcttt
   100441 acctggaaca tggacggcgt cttgaaaact gttgaaaaga ccacacgtac tttacaactg
   100501 tttctgacgc tggtggcggt gatttcgctg gtggtgggtg gtattggtgt aatgaatatt
   100561 atgctggtgt cagtgaccga gcggacgcgg gaaattggca ttcgcatggc tgtaggtgcg
   100621 cgagcaagcg atgttttgca acagttcctg atcgaagccg tactggtttg cctggtcggt
   100681 ggcgcgttgg gaataactct gtcactgtta attgctttca ccttgcagct tttcttaccc
   100741 ggctgggaga ttggtttttc accgttggcg ctgctgctgg cgtttctctg ctcgacggtc
   100801 accgggattt tatttggctg gttacccgca cgaaatgcgg cacgactgga tccagtagat
   100861 gctctggcac gagagtaatt tttgagataa aaatgccagc cgatcgggct ggcattctgc
   100921 ctttaggatg tacacaatga gacagaagag ctatgcgact gccgcttcta cttcgacggg
   100981 cacaataaca ctggcgtgat tgccttttgg cccctggtgg acatcaaact gaacggattg
   101041 tccagctttt agcgttctgt aaccatccat ctgaatggtg gaataatgag cgaaaatatc
   101101 ttcgccgccg ccttcagggc agatgaaacc aaaccctttg gcattgttga accacttaac
   101161 agtacccttt tccatgcttc gacatccttc gcaaatctta tacaagtaag atggaataaa
   101221 ccggggtcag agagggggct gttcaaaacc tcgccaactc tagaaataca atttagagaa
   101281 ttagggcgag ccgtcaagcg tttgacaggg gccagggggc aggtatgaat caaaaatttg
   101341 aagcagttaa cgctattgac aggaatgtga cagatgtcgc tgatgccaac gatagatgat
   101401 agttatctat catgtggagt agattggtca ggcaaataag ctcttgtcag cggcagggcg
   101461 ttctgccgat aaccgtaacc gaagatgata actgacaatg ggtaaaacga acgactggct
   101521 ggactttgat caactggcgg aagaaaaagt tcgcgacgcg ctaaaaccgc catctatgta
   101581 taaagtgata ttagtcaatg atgattacac tccgatggag tttgttattg acgtgttaca
   101641 aaaattcttt tcttatgatg tagaacgtgc aacgcaattg atgctcgctg ttcactacca
   101701 ggggaaggcc atttgcggag tctttaccgc cgaggttgca gaaaccaaag tggcgatggt
   101761 gaacaagtac gcgagggaga atgagcatcc attgctgtgt acgctagaaa aagcctgaat
   101821 gcaggcataa aaattggggg aggtgcctat gctcaatcaa gaactggaac tcagtttaaa
   101881 tatggctttc gccagagcgc gcgagcaccg tcatgagttt atgaccgtcg agcacttgtt
   101941 actggcgctg ctcagtaacc catctgcccg ggaggcgctg gaagcgtgtt ctgtggattt
   102001 ggttgcgctc cgtcaggaac tggaagcctt tattgaacaa accacacccg ttctgcctgc
   102061 cagtgaagag gagcgcgaca cacagccgac gctgagtttt cagcgtgtac tgcaacgtgc
   102121 ggtcttccat gtccagtcct ccggtcgcaa tgaggtaacc ggtgcaaacg ttctggtcgc
   102181 tatctttagc gaacaggagt cgcaggcggc atatctgttg cgtaaacatg aagtcagccg
   102241 tctcgatgtg gtgaatttta tctctcatgg cacgcgtaaa gacgagccga cacagtcttc
   102301 tgatcctgac agccagccaa acagcgaaga acaagctggt ggggaggaac gtatggagaa
   102361 tttcacgacg aacctgaatc agcttgcgcg cgtgggcgga atcgacccac tgattggtcg
   102421 tgagaaggag ctggagcgtg ctattcaggt tctctgccgt cgccgtaaaa acaacccgct
   102481 gctggtgggg gaatctggtg tcggtaaaac cgcgattgca gaaggtcttg cctggcgaat
   102541 tgttcagggc gatgtgccgg aagtgatggc tgactgtacg atttactctc tcgatatcgg
   102601 ttctctgtta gcgggcacaa aatatcgcgg cgactttgaa aaacgtttta aagcgttgct
   102661 caagcagctg gagcaggaca ctaacagcat cctgtttatt gatgagatcc acaccattat
   102721 cggtgcgggg gcagcgtctg gtggtcaggt cgatgcggct aacctaatca aaccgttgct
   102781 ctccagcggt aaaattcgtg taattggttc gacaacctat caggagttca gcaacatttt
   102841 cgagaaagac cgtgctctgg cgcgtcgctt ccagaaaatt gatattactg aaccgtcaat
   102901 cgaagaaact gttcaaatca tcaatggcct gaaaccgaag tatgaagcgc accacgacgt
   102961 gcgttatacc gcaaaagcgg tgcgtgcggc ggtagagctg gcggtgaaat acatcaatga
   103021 tcgtcatctg ccggataaag cgattgatgt tatcgacgaa gcgggcgctc gcgcacgcct
   103081 gatgccggta agcaaacgca agaaaaccgt taatgtggcg gatattgagt ccgtggtggc
   103141 ccgtattgca cgcattccag agaagagtgt ttctcagagt gaccgcgata ccctgaaaaa
   103201 cctcggcgat cgcctgaaaa tgctggtctt cggtcaggat aaagccattg aggcgctgac
   103261 tgaagccatt aagatggcgc gtgcaggttt aggtcacgaa cataaaccgg tcggttcgtt
   103321 cctgtttgcc ggtcctaccg gggtcgggaa aacagaggtg acggtacagc tttcgaaagc
   103381 tttgggcatt gagcttctgc gctttgatat gtccgagtat atggaacgcc ataccgtcag
   103441 ccgtctgatt ggtgcgcctc cgggatacgt tggttttgat cagggcggtt tgctgactga
   103501 tgcggtcatc aagcatccac atgcggttct gctgctggac gaaatcgaga aagcgcaccc
   103561 tgacgtgttc aatattctgt tgcaggtgat ggacaacggt acgctgaccg ataacaacgg
   103621 acgcaaagcg gacttccgta acgtggtgct ggtgatgacc accaacgccg gggtacgtga
   103681 aactgagcgt aaatccattg gtcttatcca ccaggataac agcaccgatg cgatggagga
   103741 gatcaagaag atctttacac cggaattccg taaccgtctc gacaacatta tctggtttga
   103801 tcatctgtca accgacgtga tccatcaggt ggtggataaa ttcatcgtcg agttgcaggt
   103861 tcagctggat cagaaaggtg tttctctgga agtgagccag gaagcgcgta actggctggc
   103921 cgagaaaggt tacgaccggg caatgggcgc acgtccgatg gcgcgtgtca tccaggacaa
   103981 cctgaaaaaa ccgctcgcca acgaactgct gtttggttcg ctggtggacg gcggtcaggt
   104041 caccgtcgcg ctggataaag agaaaaatga gctgacttac ggattccaga gtgcacaaaa
   104101 gcacaaggcg gaagcagcgc attaatctga ttgttaggta ggttggtcaa gtccgtaatc
   104161 tcgaaagagg ttacggactt tttgtttatg gggtggagga ggttcagacc ctttttttaa
   104221 tgatgatggt aagttgttga taattagtgc tgcgggaagg taaggataaa aaagggtgct
   104281 gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag tatttgagtt
   104341 cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc ccaccatgtt
   104401 cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta cgagctcttc
   104461 tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta tacacacttt
   104521 ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta ccaaacctca
   104581 tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga agaattttaa
   104641 tgataattat tgtttgctca tactgtaaac aagttgtgca gtatatctac atcgagacaa
   104701 gttacggact tatacttcca aagtacttca tacatatcac aaaataaaaa ggccggttaa
   104761 accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat cagcgactac
   104821 ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc actttgtcgc
   104881 ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca gtaaccacgt
   104941 gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga acggtacctt
   105001 gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta ccgtaatttg
   105061 aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc taaatcgcaa
   105121 caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa aagcgcaacg
   105181 tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt ccttatcggc
   105241 agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg gggagaaatg
   105301 tgccgaaaac attcattctt gtggtgaaaa caagcatcgt ggtacccaga aattattcgg
   105361 caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg gaatttcgca
   105421 ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct taccgccatg
   105481 accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt ccatccggct
   105541 gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac cgccgacaag
   105601 ctcatcttca cgccagactt caatggagtg ggcatggccg agttcgtgaa ggcgatggta
   105661 ggcttcgacc acgccacgcg tgatccaggt tccttctttg cgatcgctgg cacagccttc
   105721 aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt tatgaaatcg
   105781 cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg gatcgggcga
   105841 ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct ggtaagccat
   105901 taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag gctcacgtaa
   105961 tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca ggcgcatgac
   106021 cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg cttgaactgg
   106081 taataacgcc cctgtctggc aagcagctct gcgtgagtac cttgctcaat aatttgcccg
   106141 ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg ggtgaccatt
   106201 aacaccgttt tctcacgcat catttctgca agcaattcaa ggatctggct ttcggttgtg
   106261 gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg taacagcgca
   106321 cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc ttcacctaac
   106381 caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg caagatctcc
   106441 gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg cagcgtggcg
   106501 ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc cgcttcattc
   106561 aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc ccatgcgcgg
   106621 gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat cgctatatgt
   106681 tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg ctccggataa
   106741 gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt ttgggtatca
   106801 gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc agaggcaatg
   106861 acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa cgcggctaac
   106921 gcgcagaaga caaacagggc aattaacgcg ccgggttgag cattgccgcc aacgccgcca
   106981 gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat tatcgcttgc
   107041 gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat ttctgtattc
   107101 tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc ttgcccttgc
   107161 agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt ttgcccggtg
   107221 ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa cataatgccg
   107281 cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac caccataatc
   107341 accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc gagcgtatca
   107401 acatccgcca ccacgcgatt gagcaattcg ccctgacgat agcgcgccag tccggcaggg
   107461 gagaggggca gcaatttgct gaaggtgtaa atgcgcagat gctgcaacac gcggaaagtc
   107521 gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat tgctgcgcca
   107581 cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac ccccgcaacc
   107641 gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc gagcagcgtg
   107701 acaattgcca gcacgatacc aagacttaac atccatttat gacgtttata cagtgccaga
   107761 tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa tgtggcgaat
   107821 gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg gccatcctgc
   107881 ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac cattaacgtt
   107941 gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact gtgagcatca
   108001 aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa cgcacgggcc
   108061 accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc aacaggtgta
   108121 tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc cagcgctgct
   108181 tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg caatgttgct
   108241 gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga ttctggtgat
   108301 aaatcgcgta attctatccc gttgattcgt aatgatccct gatatgagag aaaaccagaa
   108361 agcgcgttca gcagtgagct tttacctgaa ccgctgcgac caaccaacac tgcacgttgg
   108421 cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg cgacgtgata
   108481 aacagatcct cggcctcaat ggtcaccgga tcggtcgatg ctaattccac ctcgccacgc
   108541 tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc accaacagcc
   108601 tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa ctctggcgca
   108661 aggatcaggg ccagaaaacc cgcagccagc gtcacgccag tatcgtagtg accaaaatcc
   108721 agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga cagcgaggta
   108781 aaaaattcga gaatgccgga ggataaaaat gccagccgta gcacttccat tgtccgttgg
   108841 cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg accaaaaata
   108901 cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg agcaagagcg
   108961 agaaagttac gtcggttagc atcggcagcc cccattccaa ccagcgccat aaacaacgga
   109021 attaacggtg cagtgcccag cagaatgagc gccgcagccc agtttgaggg gaatattgcc
   109081 accacaatca gcaacggcac cgacactgcc agcgccattt gcggcaggta gcgtgcatag
   109141 taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc cgcaggttta
   109201 ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgccg gcggatggca
   109261 aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac cacccatgcg
   109321 cgcagtacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc acggggaata
   109381 ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc aatgatcaat
   109441 atgccgctca caaagcccag cagacgagaa atattcagcc aacgttggga gatgacgctt
   109501 tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc acgcttagca
   109561 ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat aaaggcgacc
   109621 catagtcgca tggtgtcgcc ttctttactt ttgttactga tttgtaaaat tattttgcgt
   109681 cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcct gtaccagcag
   109741 atgtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag acgccaggaa
   109801 tgctggtctg ggtggcatta ccatgaatac ccgactgcac tttgatgtag ccgttttcca
   109861 gttccagctg cccttcgaaa atggcagtat tcgggctgtg accgatagca acaaacagac
   109921 cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga cgaacgccag
   109981 tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc agaatgatgt
   110041 tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg cggaaaccgt
   110101 cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc gcctcttcaa
   110161 ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag aaaccgtcgc
   110221 aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc aggccgagat
   110281 agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg ccgttatcgc
   110341 cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca aaaatgatct
   110401 cagtttcaaa cttggtggca tgttcgtgca tgcgctccat taataacgga ccggtcagat
   110461 cgtttggatc gccaggccag ttttccactt ccgtggtggt ggtcagttgg ccgccttttt
   110521 ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca gcagcggtgt
   110581 atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg cccatgagat
   110641 ccccatagtt gttggcagac aatgggctgg attgtaggga atttacagac gtaaaaaaag
   110701 agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca ggtcaaattc
   110761 tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat taaatgatga
   110821 ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg ttttgctttg
   110881 acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaaacagta ttcttatatg
   110941 cgcataacca tgcatgtaaa taccatgttt accgtgctag tgaaatctac gtatggcgtg
   111001 gacagacgcc attcgtgatg tcgatagctg ccacaaggca acggtcttct caccgtagac
   111061 ccaggcattg cgcgccgtga atcttcatga tttcggtcta tcgtgacggg tagcgactct
   111121 gaacagtgat gtttcagggt cagacaggag tagggaagga atacagagag acaataataa
   111181 tggtagatag caagaagcgc cctggcaaag atctcgaccg tatcgatcgt aacattctta
   111241 atgagttgca aaaggatggg cgtatttcta acgtcgagct ttctaaacgt gtgggacttt
   111301 ccccaacgcc gtgccttgag cgtgtgcgtc ggctggaaag acaagggttt attcagggct
   111361 atacggcgct gcttaacccc cattatctgg atgcatcact tctggtattc gttgagatta
   111421 ctctgaatcg tggcgcaccg gatgtgtttg aacaattcaa taccgctgta caaaaacttg
   111481 aagaaattca ggagtgtcat ttagtatctg gtgatttcga ctacctgttg aaaacacgcg
   111541 tgccggatat gtcagcttac cgtaagttgc tgggggaaac cctgctgcgt ctgcctggcg
   111601 tcaatgacac acggacatac gttgttatgg aagaagtcaa gcagagtaat cgtctggtta
   111661 ttaagacgcg ctaacacgga acaggtgcaa aatcggcgta ttttgattac actcctgtta
   111721 atccatacag caacagtact ggggtaacct ggtactgttg tccgttttag catcgggcag
   111781 gaaaagcctg taacctggag agcctttctt gagccaggaa tacactgaag acaaagaagt
   111841 cacattgaca aagttaagta gcggccgccg ccttctggag gcgttgctga tccttattgt
   111901 cctgtttgcc gtctggttga tggctgcctt actaagcttt aacccttcgg accccagctg
   111961 gtcgcaaacg gcctggcatg aacctatcca taatttaggt gggatgcccg gtgcgtggtt
   112021 ggcagatacg ctgttcttta tttttggcgt gatggcttac accattcccg tcattattgt
   112081 cggcggttgt tggtttgcct ggcgtcatca gtccagcgac gaatacattg attattttgc
   112141 cgtttcgcta cgcatcattg gcgttttggc gctcatcctt acctcctgtg gtctggcggc
   112201 aatcaacgct gacgatatct ggtattttgc ctccggtggc gtcattggca gcttactaag
   112261 cactacgcta caaccactgc tacacagtag cgggggaact attgcgctgc tctgcgtttg
   112321 ggcagcgggc ctgacgttgt tcaccggttg gtcatgggtg accattgctg aaaaactcgg
   112381 cggctggatt ttaaacattc tcaccttcgc cagtaatcgt acccgtcgcg atgatacctg
   112441 ggtcgatgaa gatgaatatg aagatgacga agagtatgaa gatgaaaacc acggcaaaca
   112501 gcatgaatca cgccgtgccc gtattcttcg cggcgcgcta gcgcgtcgta aacggttggc
   112561 ggaaaaattc attaatccga tggggcggca aacagacgct gcgttgttct ccggtaagcg
   112621 gatggatgat gacgaagaga ttacctacac tgcacgcggt gtggctgccg acccggacga
   112681 cgtcctattt tcgggcaatc gtgcaacgca gccagaatat gacgaatacg atccattatt
   112741 aaacggtgcg ccaattaccg aacctgtcgc tgtggcagct gctgctacca cggcgacaca
   112801 aagctgggct gcgccggttg aacctgtgac tcagacgccg cctgttgcct ctgttgatgt
   112861 tccaccttcg caacctacag tagcctggca gcctgtaccg ggtccacaaa cgggagagcc
   112921 ggttattgct cctgcaccgg aaggttaccc acagcagtca caatatgcgc agcctgcagt
   112981 gcaatataat gagccgctgc aacaaccagt acagccgcag cagccgtatt atgcacctgc
   113041 agctgaacaa cctgcgcaac aaccttacta cgctcctgcg gcggaacaac ctgttcagca
   113101 accgtattac gcccctgcgc cagaacaacc ggtggcaggt aacgcctggc aagccgaaga
   113161 gcagcaatcc acttttgctc cacagtctac ataccagact gagcaaactt atcagcaacc
   113221 agccgctcag gagccgttgt accaacagcc gcaatccgtt gaacagcagc ctgttgtgga
   113281 gcctgaaccc gttgtagaag agacaaaacc cgcgcgtccg ccgctttact attttgaaga
   113341 agtggaagag aagcgagccc gtgaacgtga acaacttgcg gcctggtatc aaccgattcc
   113401 agaaccggtt aaagaaccag aaccgatcaa atcttcgttg aaagcgcctt ctgttgcagc
   113461 agtacctcca gtagaagccg ctgccgctgt ttctccgctg gcatctggcg tgaaaaaagc
   113521 gacactggcg acgggggctg ccgcaaccgt tgccgcgcca gtcttcagtc tggcaaatag
   113581 cggtggaccg cgtccgcaag tcaaagaggg gattggcccg cagttgccac gaccgaaacg
   113641 tatccgcgtg ccaactcgtc gtgaactggc gtcttacggt attaagctgc cctcacagcg
   113701 tgcggcggaa gagaaagccc gtgaagccca gcgcaatcag tatgattctg gcgatcagta
   113761 taacgatgat gaaatcgatg cgatgcagca ggatgaactg gcacgtcagt tcgcccagac
   113821 acagcagcaa cgctatggcg aacagtatca acatgatgtg cccgtaaacg cagaagatgc
   113881 agatgctggg gcagaggctg aactggctcg tcagtttgcg caaactcaac aacaacgtta
   113941 ttccggcgaa caaccggctg gggcgaatcc gttctcgctg gatgattttg aattttcgcc
   114001 aatgaaagcg ttgctggatg atggtccaca cgaaccgttg tttacgccaa ttgttgaacc
   114061 tgtacagcag ccgcaacaac cggttgcacc gcagcagcag tatcagcagc cgcaacaacc
   114121 agttccgcca cagccgcaat atcagcagcc gcaacaacca gttgcgccgc agcagcagta
   114181 tcagcagccg caacaaccgg ttgcaccgca gccgcaggat accctgcttc atccgctgtt
   114241 gatgcgtaat ggcgacagcc gtccgttgca taaaccgacg acgccgctgc cttctctgga
   114301 tttgctgaca ccgccgccga gcgaagtgga gccggtagat acctttgcgc ttgaacaaat
   114361 ggctcgcctg gtggaagcgc gtctggctga tttccgtatt aaagccgatg tcgtcaatta
   114421 ctctccgggg ccggttatca ctcgctttga acttaacctg gctcctggcg taaaagcggc
   114481 gcgcatttct aacttgtcac gggaccttgc ccgttcactt tcgacggtgg cggtgcgtgt
   114541 cgttgaagtt attcctggca aaccctatgt aggtctggag ttaccaaata aaaaacgaca
   114601 aaccgtttat ctgcgcgaag ttttggataa cgccaaattc cgcgataatc cgtcgccatt
   114661 aaccgtggtg ctgggtaaag atatcgccgg tgagccggtg gttgccgatc tggcgaaaat
   114721 gccgcacttg ttggttgcgg ggactaccgg ttccggtaaa tctgtcggtg tgaacgcgat
   114781 gatcctgagc atgctttata aagcacagcc agaagatgtg cgtttcatca tgatcgaccc
   114841 gaaaatgctg gagctttcgg tttatgaagg cattccgcat ctgttaacgg aagtcgttac
   114901 tgatatgaaa gatgccgcca acgcgctgcg ctggtgtgtt aacgagatgg agcgtcgtta
   114961 taaactgatg tctgcgctgg gtgtgcgtaa tctggcgggt tataacgaaa aaattgctga
   115021 agccgattgc atgatgcgtc cgattccaga cccgtactgg aagccgggtg acagtatgga
   115081 tgcccagcat ccggtgctga aaaaagaacc atacattgtg gtgttggttg acgaatttgc
   115141 cgacctgatg atgacggtag gtaaaaaagt ggaagagctg atagcacgtc tggcgcaaaa
   115201 agcccgtgcc gcgggtatcc acctcgtact ggcaactcag cgtccatcgg ttgatgttat
   115261 tactggtctg attaaagcga atattccgac ccgtatcgcc tttaccgtat ccagtaagat
   115321 tgactcacgt accattcttg atcaggctgg cgcggaatca ctgctgggga tgggggatat
   115381 gctctactct gggccgaact ccacgttgcc ggtacgtgtc catggtgctt ttgttcgcga
   115441 tcaggaagtt catgccgtgg tgcaggactg gaaagcgcgt ggtcgcccac agtatgttga
   115501 tggcatcact tccgacagtg aaagcgaagg tggcgcgggc ggtttcgatg gcgctgaaga
   115561 actggatccg ttgttcgatc aggcggtgca gtttgtcact gaaaaacgca aagcgtcaat
   115621 ttctggcgta cagcgtcagt tccgcattgg ttataaccgt gcagcgcgta ttatcgaaca
   115681 gatggaagcg caggggattg tcagcgaaca ggggcacaac ggtaatcgtg aagtgctggc
   115741 cccaccgccg tttgactaat taatgcattg ccggataagg cgcggtagcg tcgcatccgg
   115801 cactctatca actgaaaatt cagtattttc ttctttcctc aagctgatta ttagcctgga
   115861 atagagagta gagggaactc ccgatcggga gtgacgtaat ttgaggaata atgatgaaaa
   115921 aaattgccat cacctgtgca ttactctcaa gcttagtagc aagcagcgtt tgggctgatg
   115981 ccgcaagcga tctgaaaagc cgcctggata aagtcagcag cttccacgcc agcttcacac
   116041 aaaaagtgac tgacggtagc ggcgcggcgg tgcaggaagg tcagggcgat ctgtgggtga
   116101 aacgtccaaa cttattcaac tggcatatga cacaacctga tgaaagcatt ctggtttctg
   116161 acggtaaaac actgtggttc tataacccgt tcgttgagca agctacggca acttggctga
   116221 aagatgccac cggtaatacg ccgtttatgc tgattgcccg caaccagtcc agcgactggc
   116281 agcagtacaa tatcaaacag aatggcgatg actttgtcct gacgccgaaa gccagcaatg
   116341 gcaatctgaa gcagttcacc attaacgtgg gacgtgatgg cacaatccat cagtttagcg
   116401 cggtggagca ggacgatcag cgcagcagtt atcaactgaa atcccagcaa aatggggctg
   116461 tggatgcagc gaaatttacc ttcaccccgc cgcaaggcgt cacggtagat gatcaacgta
   116521 agtagaggca cctgagtgag caatctgtcg ctcgattttt cggataatac ttttcaacct
   116581 ctggccgcgc gtatgcggcc agaaaattta gcacagtata tcggccagca acatttgctg
   116641 gctgcgggga agccgttgcc gcgcgctatc gaagccgggc atctgcattc tatgatcctc
   116701 tgggggccac cgggtaccgg caaaacaact ctcgctgaag tgattgcccg ctatgcgaac
   116761 gctgatgtgg aacgtatttc tgccgtcacc tctggcgtga aagagattcg cgaggcgatc
   116821 gagcgcgccc ggcaaaaccg caatgcaggt cgccgcacta ttctttttgt tgacgaagtt
   116881 caccgtttca acaaaagcca gcaggatgca tttctgccac atattgaaga cggcaccatc
   116941 acttttattg gcgcaaccac tgaaaacccg tcgtttgagc ttaattcggc actgctttcc
   117001 cgtgcccgtg tctatctgtt gaaatccctg agtacagagg atattgagca agtactaact
   117061 caggcgatgg aagacaaaac ccgtggctat ggtggtcagg atattgttct gccagatgaa
   117121 acacgacgcg ccattgctga actggtgaat ggcgacgcgc gccgggcgtt aaatacgctg
   117181 gaaatgatgg cggatatggc caaagtcgat gatagcggta agcgggtcct gaagcctgaa
   117241 ttactgaccg aaatcgcagg tgaacgtagt gcccgctttg ataacaaagg tgatcgcttt
   117301 tacgatctca tttccgcact gcataaatcg gtacgtggta gcgcacccga tgcggcgctg
   117361 tactggtatg cgcgaattat tactgccggt ggcgatccgt tatatgttgc gcgtcgctgt
   117421 ctggcgattg cgtctgaaga tgtgggtaat gctgatccac gagcgatgca ggtggcaatt
   117481 gcagcctggg attgctttac tcgcgttggc ccggcggaag gtgaacgcgc cattgctcag
   117541 gcgattgttt acctggcctg cgcgccaaaa agcaacgctg tctacactgc gtttaaagct
   117601 gcgctggcgg atgctcgcga acgtccggat tatgacgtgc cggttcattt gcgtaatgcg
   117661 ccgacgaaat taatgaagga aatgggctac gggcaggaat atcgttacgc tcatgatgaa
   117721 gcaaacgctt atgctgccgg tgaggtttac ttcccgcggg aaatagcaca aacacgctat
   117781 tatttcccga caaacagggg ccttgaaggc aagattggcg aaaagctcgc ctggctggct
   117841 gaacaggatc aaaatagccc cataaaacgc taccgttaat gttatcgttg cggtaatgtt
   117901 gttactgtat ccctgtggtc gcaggctgtg gccacatctc ccatttaatt cgataagcac
   117961 aggataagca tgctcgatcc caatctgctg cgtaatgagc cagacgcagt cgctgaaaaa
   118021 ctggcacgcc ggggctttaa gctggatgta gataagctgg gcgctcttga agagcgtcgt
   118081 aaagtattgc aggtcaaaac ggaaaacctg caagcggagc gtaactcccg atcgaaatcc
   118141 attggccagg cgaaagcgcg cggggaagat atcgagcctt tacgtctgga agtgaacaaa
   118201 ctgggcgaag agctggatgc agcaaaagcc gagctggatg ctttacaggc tgaaattcgc
   118261 gatatcgcgc tgaccatccc taacctgcct gcagatgaag tgccggtagg taaagacgaa
   118321 aatgacaacg ttgaagtcag ccgctggggt accccgcgtg agtttgactt tgaagttcgt
   118381 gaccacgtga cgctgggtga aatgcactct ggcctcgact ttgcagctgc agttaagctg
   118441 actggttccc gctttgtggt aatgaaaggg cagattgctc gcatgcaccg cgcactgtcg
   118501 cagtttatgc tggatctgca taccgaacag catggctaca gtgagaacta tgttccgtac
   118561 ctggttaacc aggacacgct gtacggtacg ggtcagctgc cgaaatttgc tggcgatctg
   118621 ttccatactc gtccgctgga agaagaagca gacaccagta actatgcgct gatcccaacg
   118681 gcagaagttc cgctgactaa cctggtgcgc ggtgaaatca tcgatgaaga tgatctgcca
   118741 attaagatga ccgcccacac cccatgcttc cgttctgaag ccggttcata tggtcgtgac
   118801 acccgtggtc tgatccgtat gcaccagttc gacaaagttg aaatggtgca gatcgtgcgc
   118861 ccagaagact caatggcggc gctggaagag atgactggtc atgcagaaaa agtcctgcag
   118921 ttgctgggcc tgccgtaccg taaaatcatc ctttgcactg gcgacatggg ctttggcgct
   118981 tgcaaaactt acgacctgga agtatggatc ccggcacaga acacctaccg tgagatctct
   119041 tcctgctcca acgtttggga tttccaggca cgtcgtatgc aggcacgttg ccgcagcaag
   119101 tcggacaaga aaacccgtct ggttcatacc ctgaacggtt ctggtctggc tgttggtcgt
   119161 acgctggttg cagtaatgga aaactatcag caggctgatg gtcgtattga agtaccagaa
   119221 gttctgcgtc cgtatatgaa cggactggaa tatattggct aatacccaat ttttctgaat
   119281 ctaaaaagcg cctgcgggcg ctttttttgt ctccctttga taccgaacaa taattactcc
   119341 tcacttacac gtaatactac tttcgagtga aaatctacct atctctttga ttttcaaatt
   119401 attcgatgta tacaagccta tatagcgagc tgctatagaa ataattacac aatacggttt
   119461 gttactggaa tcaatcgtga gcaagcttga gtgagccatt atgaaaacga aaatccctga
   119521 tgcggtattg gctgctgagg tgagtcgccg tggtttggta aaaacgacag cgatcggcgg
   119581 cctggcaatg gccagcagcg cattaacatt accttttagt cggattgcgc acgctgtcga
   119641 tagcgccatt ccaacaaaat cagacgaaaa ggttatctgg agcgcctgta cagttaactg
   119701 tggtagtcgc tgcccgctac gtatgcacgt cgtggacggt gaaatcaaat atgtcgaaac
   119761 ggacaatacc ggcgatgaca attacgacgg cctgcaccag gttcgcgcct gtctgcgtgg
   119821 acgttccatg cgtcgccgtg tctataatcc ggaccgcctg aaatatccga tgaaacgagt
   119881 cggggcgcgc ggtgaaggca aatttgagcg cattagctgg gaagaagcct acgacatcat
   119941 cgcgaccaat atgcagcgcc tgatcaaaga gtacggcaac gaatccatct atctgaacta
   120001 tggcaccggt acgctgggcg gcaccatgac ccgctcctgg cccccgggaa ataccctggt
   120061 cgcgcgcctg atgaactgct gcggcggtta tctgaaccat tacggtgact actcttccgc
   120121 gcaaattgcg gaaggtttga actataccta cggcggctgg gcagatggta acagcccgtc
   120181 ggatatcgaa aacagtaaac tggtagtgct gtttggtaat aaccctggcg aaacgcgaat
   120241 gagtggcggt ggggtgactt actatcttga acaggcacgc cagaaatcta atgccagcat
   120301 gatcatcatc gatccgcgct ataccgacac cggtgccggg cgcgaagatg agtggatccc
   120361 tattcgtccg ggaacagatg ccgcactggt taacggtctg gcgtatgtca tgatcaccga
   120421 aaacctggtg gatcaggcat tcctcgataa atattgcgtt ggctacgacg agaaaacact
   120481 gccagccagt gcgccgaaaa atggtcacta taaagcttat attctgggtg aagggccaga
   120541 tggcgtggct aaaacgccgg aatgggcctc gcaaatcact ggtgttccgg cagacaaaat
   120601 catcaaattg gctcgtgaaa tcggtagtac caaaccggcg tttatcagcc agggatgggg
   120661 cccgcagcgt cacgctaacg gtgaaatcgc aacccgtgct atctcgatgc tggcgattct
   120721 gaccggtaac gttggtatta acggaggcaa cagcggcgcg cgtgaaggtt catacagctt
   120781 accgtttgtc cgtatgccga ccttggaaaa cccgatccag accagcattt cgatgtttat
   120841 gtggaccgat gccattgaac gtggcccgga aatgacggcg ctgcgtgatg gtgtacgcgg
   120901 gaaagataag ctggatgtgc cgatcaaaat gatctggaac tatgccggta actgcctgat
   120961 taaccagcat tctgaaatca accgtaccca tgaaatcctt caggatgata agaagtgcga
   121021 gctgattgtg gttatcgact gccacatgac ctcatcggcg aaatatgctg acatcctgct
   121081 gcctgactgc accgcttccg aacagatgga ctttgcgctg gatgcatcct gcgggaatat
   121141 gtcttacgtg attttcaacg atcaggtgat taaaccgcgc tttgaatgta agaccatcta
   121201 tgaaatgacc agcgaactgg caaaacgtct tggcgttgag caacagttta ctgaaggccg
   121261 tacccaggaa gagtggatgc ggcatctgta tgcccagtcg cgggaagcga ttcctgaact
   121321 gccaacgttt gaagagttcc gcaagcaggg gatctttaaa aagcgcgacc cacaagggca
   121381 tcacgttgct tataaagcct tccgtgaaga tccgcaggca aacccactga ctacgctatc
   121441 gggcaaaatt gagatttatt cgcaggcgct ggctgacatt gccgctacct gggaattgcc
   121501 tgaaggcgat gtgatcgatc cactgccgat ctacacgccg ggctttgaaa gttatcagga
   121561 tccgctgaac aaacagtatc cgctgcagct tacaggtttc cactataaat ctcgcgttca
   121621 ctcaacttac ggcaacgttg atgtgctgaa agcggcttgc cgtcaggaaa tgtggatcaa
   121681 cccgcttgat gcccaaaaac gcggtatcca caacggcgat aaagtcagga tctttaacga
   121741 tcgtggtgag gttcatattg aggcgaaagt gacgccacga atgatgccgg gtgtggtcgc
   121801 actgggtgaa ggtgcctggt atgacccgga tgcaaaacgt gtcgataagg gtggttgtat
   121861 taacgtactg accactcaac gtccgtctcc tctcgctaag gggaatccgt cacatacaaa
   121921 ccttgttcag gttgaaaagg tgtaaggagt aaccgatgac aacccagtat ggatttttta
   121981 ttgattccag ccgttgcacc ggttgcaaaa cctgcgagct ggcttgtaaa gactacaaag
   122041 atttgacgcc agaagtcagc ttccgccgca tttatgaata tgctggcggc gactggcagg
   122101 aagataacgg tgtctggcac cagaacgtgt ttgcctacta tctgtcgatt tcgtgcaacc
   122161 actgcgaaga tccggcttgt actaaagtct gcccgagcgg tgcgatgcat aaacgtgaag
   122221 atggttttgt tgtggtcgat gaagatgtgt gcattggctg ccgctactgc catatggctt
   122281 gcccgtatgg cgcaccgcaa tataacgaaa cgaaaggcca tatgaccaaa tgcgatggtt
   122341 gttatgaccg tgttgcggag ggtaaaaagc cgatctgtgt tgaatcttgt ccgctgcggg
   122401 cgctggattt cggccctatc gacgagctgc gtaaaaaaca tggcgatctg gcggccgttg
   122461 cgccgttgcc gcgagctcac tttaccaaac cgaatattgt gatcaaaccc aatgccaata
   122521 gccgcccgac cggggatacc actggctatc tggcaaaccc gaaggaggtg tgagatggga
   122581 agtggatggc atgaatggcc gctgatgatc ttcacggtct tcgggcaatg tgtggcgggt
   122641 ggttttatcg ttctggcttt ggcgctgctc aaaggcgacc tgcgagcaga agcccagcag
   122701 cgtgttatcg cctgcatgtt tggtttatgg gtgctgatgg gcattggctt tatcgcctct
   122761 atgctccatc ttggttcacc aatgcgcgct tttaactcgc tcaaccgggt aggggcttca
   122821 gcactcagta acgaaatcgc cagcggttcg atcttttttg ccgtaggcgg catcggctgg
   122881 ttgctggcaa tgctgaaaaa gctgtcaccg gcattgcgta cgctgtggct gatagtgacg
   122941 atggttcttg gcgtcatctt tgtctggatg atggtgcgtg tgtataacag cattgatacc
   123001 gttccgacct ggtacagcat ctggacgccg atgggcttct tcctgacgat gtttatgggc
   123061 ggcccgttac tgggttacct gttgttgagt ctggctggcg tcgatggctg ggcgatgcgt
   123121 ctgctgccag caatttcttt actggcactg gtagtgagtg gcgtggtgtc agtgatgcag
   123181 ggcgcagagc tggcgactat tcatagttct gtgcagcagg ccgcagcgct ggtgccggac
   123241 tatggtgcgc tgatgtcctg gcggatcgtg cttttggccg ttgccctgtg cttgtggatt
   123301 gcaccacagc taaaaggtta tcagcctgcg gttccgctac tttctgtatc attcattctg
   123361 cttctggcag gggaattaat cggtcgcggc gtattctatg gtttgcatat gaccgtgggg
   123421 atggccgtcg caagctaatc ataacaaccg gggtttcggc cccggttctc ttttatttct
   123481 gcttcgttaa cgtgtcataa ctggtcatca aattacgata atcaggaatg tggttagaga
   123541 acaacgtcgc cagtccttca atatcattac gccagtcgcg atgcagctcg cacgccacgc
   123601 cgaaccatgt catcaattgc gcaccagctt gcgacatgcg atcccatgcc gaatgccggg
   123661 taatttcatt aaaggtccct gatgcgtcag tcacaacaaa cacatcaaac ccttcttcaa
   123721 tggctgaaag cgccgggaat gccacgcaaa cttcggttac cacaccggca ataattaact
   123781 gttttttacc tgtcgctttg actgctttta caaaatcttc gttatcccag gcgttaatat
   123841 tgcccgggcg agcaatgtaa ggtgcatcgg ggaattgtgc tttcagttct ggaactaatg
   123901 ggccgttagg accggtttca aaactggtgg taagaatagt tggtaagttg aaatacttag
   123961 ccaggtcacc cagcgccagc acgttatttt taaacttatc gggttcgata tcccgtacaa
   124021 gggaaagtaa accagcctgg tgatcaacaa gcaaaacggc agcatcattt ttatcaagac
   124081 gaacatacgg tttggtcatc ctcttctcct ttcgagagtc agtggtacgt ctgaaatcga
   124141 tcagacgcga tgcattgctc tgaaagcata gacgggaaat atgagtttgc tgtgaccatg
   124201 aaatttttcg actgaacgaa gcgtatgaaa tttgtgttag ttcaataaaa acaatcagat
   124261 gagactaatc atctcttcag cattagcgtt atgaatgagt aatataaatc gataataccg
   124321 ccgctgggat atcgcgtatt ttcacccatt gacaatgttt ttggcggtgg catgatgcgc
   124381 atgaaatttg aacttcctca aggttttaat tcatgtccac gtatacccgg cctgtcatgc
   124441 ttttgctgtc tggcctgctt ttgttgactc tggcgattgc ggtgttaaat acactcgtgc
   124501 cgctttggct cgcccaggaa cacatgtcca catggcaggt aggcgttgtc agctcatcct
   124561 attttaccgg caaccttgtc ggtacattgc tgacagggta tgtcattaag cgcattggct
   124621 ttaaccgcag ctattatctg gcctccttca tttttgccgc tggctgtgcc ggccttggcc
   124681 tgatgattgg attctggagc tggttggctt ggcgttttgt cgcgggcgtc ggctgtgcca
   124741 tgatttgggt ggttgttgag agcgcgctga tgtgcagtgg gacgtcacgt aaccgtgggc
   124801 gtttgcttgc tgcgtatatg atggtttatt acgtgggaac gtttttaggc cagttactgg
   124861 tcagcaaagt ttcaaccgag ctgatgtccg tattgccgtg ggttacaggt ttgacgttgg
   124921 cagggatctt accgctgttg tttacgcgtg tgctgaatca gcaggctgaa aaccatgatt
   124981 cgacgtcaat tacgtcaatg ctaaaactcc gtcaggcgcg gcttggcgtg aatggctgca
   125041 ttatctcagg aatcgttctg ggatctctat atggcctgat gccgctgtac ctcaatcaca
   125101 aaggggtgag caatgccagc attggtttct ggatggcggt actggtcagt gcgggtatcc
   125161 ttggacaatg gccgattgga cgtctggcgg ataagtttgg tcgactgctg gtgttgcgtg
   125221 ttcaggtctt tgtcgtcatt ctcggcagta tcgcgatgct tagccaggcg gcgatggccc
   125281 cagcgttatt catcctcggt gccgctggct ttacgctata tccggtggcg atggcatggg
   125341 cttgcgagaa agttgaacat catcaactgg tggcgatgaa ccaggcctta ctgttgagct
   125401 atactgtggg aagtctgctt ggcccgtcat ttaccgctat gctaatgcag aatttctccg
   125461 ataatttatt gtttatcatg atcgccagcg tatcgtttat ctatttgctg atgctgctgc
   125521 gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta aatgaattca agcagagtgt
   125581 gaacttactg tttcacactc tgcttttttg tttcttctat ctgacttgct ttattccaaa
   125641 ttttattcgt ttaaaaataa aatgtgcagc aggttataat tttgcatttc gctatttccg
   125701 cacttcttat ttgccgcgca taatccctcg ttttacagat gcccctttaa ttttggcgaa
   125761 ggatttgtct atggctggga atgttcagga aaaacagttg cgatggtaca acattgcgct
   125821 gatgtctttt atcactgtct ggggttttgg caacgttgtt aataactatg ccaaccaggg
   125881 gctggtggtt gttttttcat gggtgtttat ctttgcactc tatttcacac cttatgcgct
   125941 aattgttggt cagttaggct cgaccttcaa agatgggaag ggcggggtca gtacctggat
   126001 taaacacacg atgggacccg gactggctta tctcgccgcg tggacctact gggtggtgca
   126061 tattccctat ctggcacaaa aaccccaggc aattctgatt gcgctcggtt gggcgatgaa
   126121 aggcgacggt tcgctaatca aagaatattc agtcgtagcg ttacaggggt taacgctggt
   126181 gctgtttatc ttctttatgt gggttgcttc acgcggtatg aaatcgctga aaatcgtcgg
   126241 ttctgtggca gggattgcga tgtttgttat gtcgctcctg tatgtggcga tggcggtaac
   126301 cgcgcctgcg attactgaag tgcatattgc gaccacaaac attacctggg aaacgttcat
   126361 tcctcatatc gactttacct acattaccac tatttcaatg ctggttttcg cggttggcgg
   126421 agcagagaag atttctcctt acgttaatca aacgcgcaac ccaggaaaag aatttccaaa
   126481 agggatgcta tgcctggcgg tgatggttgc ggtttgtgcc attctgggct cactggcgat
   126541 ggggatgatg tttgactcgc gtaatatccc ggatgactta atgacgaacg gtcaatatta
   126601 cgcctttcag aagctgggcg agtattacaa catgggtaat actttaatgg tgatttacgc
   126661 cattgcgaat accctgggac aagtagctgc gctggtattc tcgattgatg ccccgcttaa
   126721 agtgctatta ggtgatgctg acagcaaata tattccagcc agtttatgtc gtaccaacgc
   126781 ttctggtacg cccgttaatg gctattttct gaccctggtg ctggtggcaa ttctgattat
   126841 gctgccgact ctcggcatcg gtgatatgaa caatctctat aaatggctgt tgaatcttaa
   126901 ctcggtagtg atgccgctgc gttatttatg ggtatttgtt gcatttattg cagtcgttcg
   126961 cctggcacag aaatataagc cggaatatgt ctttattcgt aataagtctc tggcaatgac
   127021 cgtcgggatt tggtgttttg cctttaccgc ctttgcctgt ttgacgggga tcttcccgaa
   127081 aatggaagca ttcaccgcag agtggacctt ccagttggcg ctcaatgttg caacgccgtt
   127141 tgtgctggtt ggattaggac tgatattccc gctgctagcg cgtaaagcga atagcaaata
   127201 attattgtgg tcggtgacgc tgactctggc agatctgacc cgtttttaca taatcgagaa
   127261 atgcgcgcaa gccgcaggac atatactggc ggtttggata atagatctgg aagcctggac
   127321 gctctgtgct ccagtctccc agtacacatt ctaaacgacc agtatcaagg tactccttga
   127381 tctgttcata gagcagatac ccgataccag cacccatcaa aacggcctcc agttcagcat
   127441 caacatcatc cagaatgata tttcctgcga cagcaatttc cagctccttt gcaaattgcc
   127501 agtggaaagg tttgccgctg ggataacgaa aaacaacaca ttgatgatta agcagatcat
   127561 gaggatggcg gggtttgcca tatcttgcaa agtactctgg cgttgctgct acacataatt
   127621 tcactggcgg gccgatcgcc acgctgatca tatctttttc gacgatgcca ctaagtcgaa
   127681 cgccagcgtc aaagccctgt tggacgatat cgacaagaga gtcgtcagtt gtgagctcga
   127741 ctttaatatc aggatattca cgtgtgaaac caaccagtaa aggcatttaa aatatacgtg
   127801 cagctacccg ggcggcattg attttgagcg ttcctgtcgg tgtcagacga aaatcattca
   127861 tctcatctaa catgatctga atttcgtcga aagctgggcg caatcgttca tagagattag
   127921 aacccgcttc cgttaacgat acgcttcggg ttgtccgatt gaataagcga attttaagac
   127981 gttgttccag tgttttaata ctatggctaa tggcggacga ggataagcct aattcatcgc
   128041 ccgctgcacg aaagctttga ttacgggcca cggcaaagaa agtggcaaag tcagacatat
   128101 tcatccgcat tagtgaaaat ccttcattaa ctcatcctga atcatcacgt ttacaggatt
   128161 atgccgcctg cgtaaagtgc gctccagaac ttaacgtgga ggtaaaatta tgcagtctga
   128221 acgtatttat ttggtatggg cccatcctcg tcatgattca ttgaccgcac atattgctga
   128281 tgcgatccat cagcgggcaa tggagcggaa aatacaggtg acggaactcg atttatatcg
   128341 gcgtaatttc aacccagtga tgacgccgga agatgaacca gactggaaga atatggataa
   128401 acgttattct ccagaggttc atcagcttta ttcagagctg cttgaacatg acacgttagt
   128461 ggtggttttt cctctctggt ggtacagctt cccggcaatg ctaaaaggat atattgacag
   128521 agtatggaat aatgggctgg cttatggaga tgggcagaaa ttaccattca ataaagttcg
   128581 ttgggtggcg ctggttggag gagacaaaga atcatttgtc cagatgggct gggaaaaaaa
   128641 tataagcgat tatttgataa atatgtgcag ttatcttggt attgaagatg ccgatgtcac
   128701 tttcttgtgt aatacagtgg tattcgatgg gcaagaactt catgcgagct attatcagtc
   128761 gttattatct caggtacggg atatggtaga tgcactataa aatgtgttaa aaacgctgta
   128821 gcagaatgaa gcgcggaata aaaaagcggc aactcaataa agttgccgct ttacggggaa
   128881 attagaacat taccttatga ccgtactgct caagaatgcc tttcacgcgt tccatggtct
   128941 ctttcttagg tggtttaaca ccgtcgagtt tgtactcttc acccattgcc acccatttgt
   129001 gtttgcccag ctcgtggtag gggaggagtt cgattttctc aacgttgccc atatcacggg
   129061 taaattcacc gaggcgatgc gctgaatcgt catcgtcaga ccagcctggg acaacaacgt
   129121 agcggatcca caccttcaca tttttgttcg ccagatattt ggcgaactcc agcgtgcggt
   129181 ggttggaaac tccaaccaga ttttggtgga tctcgtcgtt catctgtttg agatcgagca
   129241 ttaccaggtc ggttacttcc agcagttcat caatcaccgg atcgtaacga cgaacaaaac
   129301 cgttggtgtc cagacaggta tgaatgcctt cttttttgca ggcgcggaac cagtcacgaa
   129361 caaactcagc ttgtaggatt gcctcaccgc cggatgcggt aacgccgccg ccggaagcgt
   129421 tcataaagtg gcgataggtc accacctctt tcattaaatc ttcaacggta acttctttac
   129481 cgccatgcgt atcccaggtg tcgcggttat gacaatacag gcagcgcatc aggcagccct
   129541 ggaaaaaggt gataaagcga atacccgggc cgtctacggt tccacaggat tcaaaggagt
   129601 gaatgcgacc aataactgac attgcggtgt ttctccagat gtggcccatc tgaggccgtg
   129661 ttggtgcgca gctcgaaggc tacgtcgagt ctgttttggc agtcacctta aagtatagat
   129721 agctgacaaa aaaggctctc gtgctaaaaa aggccccact ttcgtggagc ctttattgta
   129781 cgctttttac tgtacgattt cagtcaaatc taattacata gattgagtga aggtacgagt
   129841 aataacgtcc tgctgctgtt ctttagtcag cgagttgaaa cgtactgcgt agccagatac
   129901 acggatggtc agctgcggat atttttccgg gttttccatc gcgtcgagca gcatttcacg
   129961 gttcatcacg ttaacgttca ggtgctgacc accttcgatg gatgcttcgt ggtggaagta
   130021 accatccatc agaccagcca ggttggtctt acgaacttcg tcgtctttac ccagtgcgtt
   130081 cggaacgata gagaaggtgt aggagatacc atctttagcg taagcaaacg gcagtttagc
   130141 aacggaagtc agagaggcta ctgcaccttt ctggtcacga ccgtgcatcg ggttagcacc
   130201 cggtccgaac ggcgcgccag cacgacgacc gtctggggtg ttaccagttt tcttaccata
   130261 cacaacgtta gaagtgatgg tcagaacaga ctgagtcggg atagcgtcac ggtaggtgtg
   130321 cagtttctga attttcttca tgaaacgttc taccaggtca acagccaggt catctacacg
   130381 cggatcgttg ttaccaaact gcgggtattc gccttcgatt tcgaagtcga tagccagacc
   130441 gtcttcgtca cgaatcggtt taactttcgc atatttgatt gcagacaggg agtcagcagc
   130501 aacggacaga ccagcgatac cacacgccat ggtgcggata acgtcacggt cgtgcagcgc
   130561 catcagagag gcttcgtagc tgtacttgtc gtgcatgtag tggatgatgt tcagtgcagt
   130621 gatgtactgt ttagccagcc agtccatgaa gtgatccatg cgctccatca cttcatcgta
   130681 gttcaggaga tcgcctttga tcggttcaga cttcggacca acctgcattt tcagtttttc
   130741 gtcaacgccg ccgttgattg cgtacagcat ggttttcgcc aggtttgcac gcgcaccgaa
   130801 gaactgcatt tgtttaccaa cgatcatcgg gcttacgcag caagcgatag catagtcatc
   130861 gttgttgaag tccggacgca tcaggtcatc gttctcatac tgcagagaag aggtgtcgat
   130921 ggacacttta gcggcgaatt tcttgaagtt cagcggcagt ttttcagacc acagaatggt
   130981 catgttcggt tccggagacg gacccatggt gtacagggtg ttcaggaaac ggaagctgtt
   131041 tttggtaacc agggtacgac cgtcgaggcc cataccaccg atagattcgg ttgcccagat
   131101 cgggtcgcca gagaacagtt catcgtattc cggagtacgc aggaagcgaa ccatacgcag
   131161 tttcatgacc aggtggtcaa ccatttcctg cgcttcttgt tcggtgatct tgccagcttt
   131221 caggtcacgt tcgatgtaca catccaggaa ggtggaggta cgaccgaagg acattgcagc
   131281 accgttctga gacttaacag cagccaggta gccgaagtaa gtccactgga tagcttcctg
   131341 agcgttggta gccggaccag agatgtcgta gccgtatttc gcagccattt ctttcatctg
   131401 acccagagcg cggtgctgtt cagcgatttc ttcgcgcaga cggatagtct gttccaggtt
   131461 tacgccgttt tccagatcag tctgcagaga ggtgaactga gcgtatttgt ctttcatcag
   131521 gtagtcgata ccgtacagcg caacgcgacg gtagtcaccg atgatacggc cacgaccata
   131581 agcatctggc agaccggtca gaacaccgga tttacggcaa cgcaggatgt ccggagtgta
   131641 aacgtcgaac acgccctggt tgtgagtttt acggtattca gtgaagattt ttttgatcat
   131701 cgggtccagt tcgcggttgt acgctttgca ggaaccttcg atcattttga taccaccgaa
   131761 cgggataaga gcacgtttca gcggagcttc agtctgcaga ccaacaactt tttccaacgc
   131821 tttgttgatg tagccagcgt cgtgagaggt gatggtggaa gcaacagcgg tgtcaaagtc
   131881 aactggcgcg tgagtgcggt tttccagttt aacgccttcc ataactttgt cccacagggt
   131941 ggtggtcgct tcagtagcgc cagccaggaa ggactcgtca ccctcgtacg gagtgtagtt
   132001 tttctgaatg aagtcacgga cgtttacttc attctgccag tcacctttgg taaaaccttc
   132061 ccaggctgtg gctaactttt cattaagctc ggacatgtaa cacctacctt cttaagtgga
   132121 ttttttattt actgcgtact tcgacaacca ttaatggtga tcgttttcac gcaggtaaat
   132181 gacccagtat gtcaacccaa ccaacaaacc accgccgata atattaccga tcgtaaccgg
   132241 gatcaggtta tcagtgatga agttcatcac ggtcaggtga gaaaaatttt ccggtgcaga
   132301 accgacagcg gtccagaatt ccggagatgc gaagtcgcgg attacaatac ccatcgggat
   132361 cataaacatg tttgcgatac tgtgctcaaa accgctggca acaaacatcg cgaccggcag
   132421 caccataatg aacgctttgt ccatcaggct gcggccagaa taactcatca atactgccag
   132481 acataccatc aggtttgcca ggataccaag acagacggcc tcaataaaag tatggtgcac
   132541 tttgtggtcg gcggtttgta ggacgtttag tccccattga ccatttgcgg tcatatactc
   132601 gccggaaagc cacattaaaa gtacaaacag cagtgcgccg accaggttgc caaaatagac
   132661 atttagccag tttttagcca actgacccca ggtgatgcgc ccactcgcct tagcaacaac
   132721 aatcaacact gtggaagtaa agagatcggc tccgcagaca acacaaagaa tcagccccag
   132781 agagaagcaa atgccgccaa ccagttttgc catgccgaag ggcattgtgc ctgtgccagt
   132841 ggttgctgtg atatagaaga cgaatgcgat tgagatgaaa acaccggcgg taatcgccag
   132901 atagaaagtc ttaagcggat gtttcgttgc tttatagaca cccgcctctt cggccacttt
   132961 ggccattgca gcaggaagta aaagatcaaa agggttgtca gctttcacac taactctctc
   133021 tttattaagt cggcgacgag atactaacaa agcattatag atgagaaatt gatatagatc
   133081 atatctcgcc tggcttatag gcccgtaact c
//
LOCUS       DAZKHC020000002       117762 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZKHC020000002 DAZKHC020000000
VERSION     DAZKHC020000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 117762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 117762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 117762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..117762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.002"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            150..2393
                     /gene="pdeF"
                     /locus_tag="ACHOSM_RS00610"
                     /old_locus_tag="pgaptmp_000122"
     CDS             150..2393
                     /gene="pdeF"
                     /locus_tag="ACHOSM_RS00610"
                     /old_locus_tag="pgaptmp_000122"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HGU4556732.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGVMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKMDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMLVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2432..3973)
                     /gene="ppx"
                     /locus_tag="ACHOSM_RS00615"
                     /old_locus_tag="pgaptmp_000123"
     CDS             complement(2432..3973)
                     /gene="ppx"
                     /locus_tag="ACHOSM_RS00615"
                     /old_locus_tag="pgaptmp_000123"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HGU4556733.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3978..6044)
                     /gene="ppk1"
                     /locus_tag="ACHOSM_RS00620"
                     /old_locus_tag="pgaptmp_000124"
     CDS             complement(3978..6044)
                     /gene="ppk1"
                     /locus_tag="ACHOSM_RS00620"
                     /old_locus_tag="pgaptmp_000124"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HGU4556734.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDVIDILFSDTVKARYIDKELSNRYVPRSNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6216..6854)
                     /gene="purN"
                     /locus_tag="ACHOSM_RS00625"
                     /old_locus_tag="pgaptmp_000125"
     CDS             complement(6216..6854)
                     /gene="purN"
                     /locus_tag="ACHOSM_RS00625"
                     /old_locus_tag="pgaptmp_000125"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGU4556735.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6854..7891)
                     /gene="purM"
                     /locus_tag="ACHOSM_RS00630"
                     /old_locus_tag="pgaptmp_000126"
     CDS             complement(6854..7891)
                     /gene="purM"
                     /locus_tag="ACHOSM_RS00630"
                     /old_locus_tag="pgaptmp_000126"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGU4556736.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGKNAWKIGIIK
                     ASDSEQRVVIE"
     gene            8215..8841
                     /gene="upp"
                     /locus_tag="ACHOSM_RS00635"
                     /old_locus_tag="pgaptmp_000127"
     CDS             8215..8841
                     /gene="upp"
                     /locus_tag="ACHOSM_RS00635"
                     /old_locus_tag="pgaptmp_000127"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGU4556737.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8927..10216
                     /gene="uraA"
                     /locus_tag="ACHOSM_RS00640"
                     /old_locus_tag="pgaptmp_000128"
     CDS             8927..10216
                     /gene="uraA"
                     /locus_tag="ACHOSM_RS00640"
                     /old_locus_tag="pgaptmp_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HGU4556738.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10311..11012
                     /gene="hda"
                     /locus_tag="ACHOSM_RS00645"
                     /old_locus_tag="pgaptmp_000129"
     CDS             10311..11012
                     /gene="hda"
                     /locus_tag="ACHOSM_RS00645"
                     /old_locus_tag="pgaptmp_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HGU4556739.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11150..11509)
                     /gene="arsC"
                     /locus_tag="ACHOSM_RS00650"
                     /old_locus_tag="pgaptmp_000130"
     CDS             complement(11150..11509)
                     /gene="arsC"
                     /locus_tag="ACHOSM_RS00650"
                     /old_locus_tag="pgaptmp_000130"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HGU4556740.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11530..12993)
                     /gene="bepA"
                     /locus_tag="ACHOSM_RS00655"
                     /old_locus_tag="pgaptmp_000131"
     CDS             complement(11530..12993)
                     /gene="bepA"
                     /locus_tag="ACHOSM_RS00655"
                     /old_locus_tag="pgaptmp_000131"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HGU4556741.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13206..14268
                     /gene="yfgO"
                     /locus_tag="ACHOSM_RS00660"
                     /old_locus_tag="pgaptmp_000132"
                     /pseudo
     CDS             13206..14268
                     /gene="yfgO"
                     /locus_tag="ACHOSM_RS00660"
                     /old_locus_tag="pgaptmp_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
     gene            complement(14306..15154)
                     /gene="focB"
                     /locus_tag="ACHOSM_RS00665"
                     /old_locus_tag="pgaptmp_000133"
     CDS             complement(14306..15154)
                     /gene="focB"
                     /locus_tag="ACHOSM_RS00665"
                     /old_locus_tag="pgaptmp_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HGU4556742.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15176..17188)
                     /gene="hyfR"
                     /locus_tag="ACHOSM_RS00670"
                     /old_locus_tag="pgaptmp_000134"
                     /pseudo
     CDS             complement(15176..17188)
                     /gene="hyfR"
                     /locus_tag="ACHOSM_RS00670"
                     /old_locus_tag="pgaptmp_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            complement(17218..17631)
                     /gene="hyfJ"
                     /locus_tag="ACHOSM_RS00675"
                     /old_locus_tag="pgaptmp_000135"
     CDS             complement(17218..17631)
                     /gene="hyfJ"
                     /locus_tag="ACHOSM_RS00675"
                     /old_locus_tag="pgaptmp_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HGU4556743.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHIGV
                     IDCLNVAFRCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17624..18382)
                     /gene="hyfI"
                     /locus_tag="ACHOSM_RS00680"
                     /old_locus_tag="pgaptmp_000136"
     CDS             complement(17624..18382)
                     /gene="hyfI"
                     /locus_tag="ACHOSM_RS00680"
                     /old_locus_tag="pgaptmp_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HGU4556744.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18379..18918)
                     /gene="hyfH"
                     /locus_tag="ACHOSM_RS00685"
                     /old_locus_tag="pgaptmp_000137"
     CDS             complement(18379..18918)
                     /gene="hyfH"
                     /locus_tag="ACHOSM_RS00685"
                     /old_locus_tag="pgaptmp_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HGU4556745.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAQQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            complement(18928..20595)
                     /gene="hyfG"
                     /locus_tag="ACHOSM_RS00690"
                     /old_locus_tag="pgaptmp_000138"
     CDS             complement(18928..20595)
                     /gene="hyfG"
                     /locus_tag="ACHOSM_RS00690"
                     /old_locus_tag="pgaptmp_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HGU4556746.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSERVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(20585..22165)
                     /gene="hyfF"
                     /locus_tag="ACHOSM_RS00695"
                     /old_locus_tag="pgaptmp_000139"
     CDS             complement(20585..22165)
                     /gene="hyfF"
                     /locus_tag="ACHOSM_RS00695"
                     /old_locus_tag="pgaptmp_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HGU4556747.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLEPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22170..22820)
                     /gene="hyfE"
                     /locus_tag="ACHOSM_RS00700"
                     /old_locus_tag="pgaptmp_000140"
     CDS             complement(22170..22820)
                     /gene="hyfE"
                     /locus_tag="ACHOSM_RS00700"
                     /old_locus_tag="pgaptmp_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HGU4556748.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22832..24270)
                     /gene="hyfD"
                     /locus_tag="ACHOSM_RS00705"
                     /old_locus_tag="pgaptmp_000141"
                     /pseudo
     CDS             complement(22832..24270)
                     /gene="hyfD"
                     /locus_tag="ACHOSM_RS00705"
                     /old_locus_tag="pgaptmp_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(24287..25234)
                     /gene="hyfC"
                     /locus_tag="ACHOSM_RS00710"
                     /old_locus_tag="pgaptmp_000142"
     CDS             complement(24287..25234)
                     /gene="hyfC"
                     /locus_tag="ACHOSM_RS00710"
                     /old_locus_tag="pgaptmp_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HGU4556749.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSMLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25245..27262)
                     /gene="hyfB"
                     /locus_tag="ACHOSM_RS00715"
                     /old_locus_tag="pgaptmp_000143"
                     /pseudo
     CDS             complement(25245..27262)
                     /gene="hyfB"
                     /locus_tag="ACHOSM_RS00715"
                     /old_locus_tag="pgaptmp_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(27262..27879)
                     /gene="hyfA"
                     /locus_tag="ACHOSM_RS00720"
                     /old_locus_tag="pgaptmp_000144"
     CDS             complement(27262..27879)
                     /gene="hyfA"
                     /locus_tag="ACHOSM_RS00720"
                     /old_locus_tag="pgaptmp_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HGU4556750.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKNGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(28132..28602)
                     /gene="bcp"
                     /locus_tag="ACHOSM_RS00725"
                     /old_locus_tag="pgaptmp_000145"
     CDS             complement(28132..28602)
                     /gene="bcp"
                     /locus_tag="ACHOSM_RS00725"
                     /old_locus_tag="pgaptmp_000145"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HGU4556751.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28602..29174)
                     /gene="gcvR"
                     /locus_tag="ACHOSM_RS00730"
                     /old_locus_tag="pgaptmp_000146"
     CDS             complement(28602..29174)
                     /gene="gcvR"
                     /locus_tag="ACHOSM_RS00730"
                     /old_locus_tag="pgaptmp_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HGU4556752.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29320..30198
                     /gene="dapA"
                     /locus_tag="ACHOSM_RS00735"
                     /old_locus_tag="pgaptmp_000147"
     CDS             29320..30198
                     /gene="dapA"
                     /locus_tag="ACHOSM_RS00735"
                     /old_locus_tag="pgaptmp_000147"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGU4556753.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30215..31249
                     /gene="bamC"
                     /locus_tag="ACHOSM_RS00740"
                     /old_locus_tag="pgaptmp_000148"
     CDS             30215..31249
                     /gene="bamC"
                     /locus_tag="ACHOSM_RS00740"
                     /old_locus_tag="pgaptmp_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HGU4556754.1"
                     /translation="MAYSVQKALLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VSVFQAAFSK"
     gene            31462..32175
                     /gene="purC"
                     /locus_tag="ACHOSM_RS00745"
                     /old_locus_tag="pgaptmp_000149"
     CDS             31462..32175
                     /gene="purC"
                     /locus_tag="ACHOSM_RS00745"
                     /old_locus_tag="pgaptmp_000149"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGU4556755.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32329..32556
                     /gene="vapB"
                     /locus_tag="ACHOSM_RS00750"
                     /old_locus_tag="pgaptmp_000150"
     CDS             32329..32556
                     /gene="vapB"
                     /locus_tag="ACHOSM_RS00750"
                     /old_locus_tag="pgaptmp_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HGU4556756.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            32556..32954
                     /locus_tag="ACHOSM_RS00755"
                     /old_locus_tag="pgaptmp_000151"
     CDS             32556..32954
                     /locus_tag="ACHOSM_RS00755"
                     /old_locus_tag="pgaptmp_000151"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4556757.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            33101..33964
                     /gene="ypfJ"
                     /locus_tag="ACHOSM_RS00760"
                     /old_locus_tag="pgaptmp_000152"
     CDS             33101..33964
                     /gene="ypfJ"
                     /locus_tag="ACHOSM_RS00760"
                     /old_locus_tag="pgaptmp_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HGU4556758.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKQVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33979..35994
                     /gene="tmcA"
                     /locus_tag="ACHOSM_RS00765"
                     /old_locus_tag="pgaptmp_000153"
     CDS             33979..35994
                     /gene="tmcA"
                     /locus_tag="ACHOSM_RS00765"
                     /old_locus_tag="pgaptmp_000153"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HGU4556759.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            36119..36766
                     /gene="ypfH"
                     /locus_tag="ACHOSM_RS00770"
                     /old_locus_tag="pgaptmp_000154"
     CDS             36119..36766
                     /gene="ypfH"
                     /locus_tag="ACHOSM_RS00770"
                     /old_locus_tag="pgaptmp_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4556760.1"
                     /translation="MLLLFHGVGDNPVAMGEIGSWFAPLFPDALVVSVGGAEPSGNPA
                     GRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGANATALIGFSQGAIMALE
                     SIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDPVIDLAHAVAAQEALISA
                     GGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEALSGGKPGDDDVIEMM"
     gene            complement(36876..37076)
                     /gene="ypfN"
                     /locus_tag="ACHOSM_RS00775"
                     /old_locus_tag="pgaptmp_000155"
     CDS             complement(36876..37076)
                     /gene="ypfN"
                     /locus_tag="ACHOSM_RS00775"
                     /old_locus_tag="pgaptmp_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HGU4556761.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(37104..38231)
                     /gene="dapE"
                     /locus_tag="ACHOSM_RS00780"
                     /old_locus_tag="pgaptmp_000156"
     CDS             complement(37104..38231)
                     /gene="dapE"
                     /locus_tag="ACHOSM_RS00780"
                     /old_locus_tag="pgaptmp_000156"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HGU4556762.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAAVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38235..38591)
                     /gene="yffB"
                     /locus_tag="ACHOSM_RS00785"
                     /old_locus_tag="pgaptmp_000157"
     CDS             complement(38235..38591)
                     /gene="yffB"
                     /locus_tag="ACHOSM_RS00785"
                     /old_locus_tag="pgaptmp_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HGU4556763.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            38972..39031
                     /gene="ypfM"
                     /locus_tag="ACHOSM_RS00790"
                     /old_locus_tag="pgaptmp_000158"
     CDS             38972..39031
                     /gene="ypfM"
                     /locus_tag="ACHOSM_RS00790"
                     /old_locus_tag="pgaptmp_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HGU4556764.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(39130..42243)
                     /gene="acrD"
                     /locus_tag="ACHOSM_RS00795"
                     /old_locus_tag="pgaptmp_000159"
     CDS             complement(39130..42243)
                     /gene="acrD"
                     /locus_tag="ACHOSM_RS00795"
                     /old_locus_tag="pgaptmp_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HGU4556765.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DCGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(42407..44107)
                     /gene="narQ"
                     /locus_tag="ACHOSM_RS00800"
                     /old_locus_tag="pgaptmp_000160"
     CDS             complement(42407..44107)
                     /gene="narQ"
                     /locus_tag="ACHOSM_RS00800"
                     /old_locus_tag="pgaptmp_000160"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HGU4556766.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            44313..46292
                     /gene="aegA"
                     /locus_tag="ACHOSM_RS00805"
                     /old_locus_tag="pgaptmp_000161"
     CDS             44313..46292
                     /gene="aegA"
                     /locus_tag="ACHOSM_RS00805"
                     /old_locus_tag="pgaptmp_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HGU4556767.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLH
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            46360..46935
                     /gene="nudK"
                     /locus_tag="ACHOSM_RS00810"
                     /old_locus_tag="pgaptmp_000162"
     CDS             46360..46935
                     /gene="nudK"
                     /locus_tag="ACHOSM_RS00810"
                     /old_locus_tag="pgaptmp_000162"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HGU4556768.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            47061..48104
                     /gene="ypfG"
                     /locus_tag="ACHOSM_RS00815"
                     /old_locus_tag="pgaptmp_000163"
     CDS             47061..48104
                     /gene="ypfG"
                     /locus_tag="ACHOSM_RS00815"
                     /old_locus_tag="pgaptmp_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HGU4556769.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(48181..50184)
                     /gene="tkt"
                     /locus_tag="ACHOSM_RS00820"
                     /old_locus_tag="pgaptmp_000164"
     CDS             complement(48181..50184)
                     /gene="tkt"
                     /locus_tag="ACHOSM_RS00820"
                     /old_locus_tag="pgaptmp_000164"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU4556770.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DLQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(50204..51154)
                     /gene="tal"
                     /locus_tag="ACHOSM_RS00825"
                     /old_locus_tag="pgaptmp_000165"
     CDS             complement(50204..51154)
                     /gene="tal"
                     /locus_tag="ACHOSM_RS00825"
                     /old_locus_tag="pgaptmp_000165"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU4556771.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            51443..53722
                     /gene="maeB"
                     /locus_tag="ACHOSM_RS00830"
                     /old_locus_tag="pgaptmp_000166"
     CDS             51443..53722
                     /gene="maeB"
                     /locus_tag="ACHOSM_RS00830"
                     /old_locus_tag="pgaptmp_000166"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HGU4556772.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            54016..54351
                     /gene="eutS"
                     /locus_tag="ACHOSM_RS00835"
                     /old_locus_tag="pgaptmp_000167"
     CDS             54016..54351
                     /gene="eutS"
                     /locus_tag="ACHOSM_RS00835"
                     /old_locus_tag="pgaptmp_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HGU4556773.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            54364..54843
                     /gene="eutP"
                     /locus_tag="ACHOSM_RS00840"
                     /old_locus_tag="pgaptmp_000168"
     CDS             54364..54843
                     /gene="eutP"
                     /locus_tag="ACHOSM_RS00840"
                     /old_locus_tag="pgaptmp_000168"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HGU4556774.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            54818..55519
                     /gene="eutQ"
                     /locus_tag="ACHOSM_RS00845"
                     /old_locus_tag="pgaptmp_000169"
     CDS             54818..55519
                     /gene="eutQ"
                     /locus_tag="ACHOSM_RS00845"
                     /old_locus_tag="pgaptmp_000169"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HGU4556775.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMLPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVIGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            55516..56319
                     /gene="eutT"
                     /locus_tag="ACHOSM_RS00850"
                     /old_locus_tag="pgaptmp_000170"
     CDS             55516..56319
                     /gene="eutT"
                     /locus_tag="ACHOSM_RS00850"
                     /old_locus_tag="pgaptmp_000170"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HGU4556776.1"
                     /translation="MKDFITEAWLRANHTLSEGVEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            56316..57332
                     /gene="pta"
                     /locus_tag="ACHOSM_RS00855"
                     /old_locus_tag="pgaptmp_000171"
     CDS             56316..57332
                     /gene="pta"
                     /locus_tag="ACHOSM_RS00855"
                     /old_locus_tag="pgaptmp_000171"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU4556777.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            57371..57664
                     /gene="eutM"
                     /locus_tag="ACHOSM_RS00860"
                     /old_locus_tag="pgaptmp_000172"
     CDS             57371..57664
                     /gene="eutM"
                     /locus_tag="ACHOSM_RS00860"
                     /old_locus_tag="pgaptmp_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HGU4556778.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            57771..58058
                     /gene="eutN"
                     /locus_tag="ACHOSM_RS00865"
                     /old_locus_tag="pgaptmp_000173"
     CDS             57771..58058
                     /gene="eutN"
                     /locus_tag="ACHOSM_RS00865"
                     /old_locus_tag="pgaptmp_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HGU4556779.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            58070..59473
                     /gene="eutE"
                     /locus_tag="ACHOSM_RS00870"
                     /old_locus_tag="pgaptmp_000174"
     CDS             58070..59473
                     /gene="eutE"
                     /locus_tag="ACHOSM_RS00870"
                     /old_locus_tag="pgaptmp_000174"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HGU4556780.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETTQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHVVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            59484..60320
                     /gene="eutJ"
                     /locus_tag="ACHOSM_RS00875"
                     /old_locus_tag="pgaptmp_000175"
                     /pseudo
     CDS             59484..60320
                     /gene="eutJ"
                     /locus_tag="ACHOSM_RS00875"
                     /old_locus_tag="pgaptmp_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
     gene            60310..61497
                     /gene="eutG"
                     /locus_tag="ACHOSM_RS00880"
                     /old_locus_tag="pgaptmp_000176"
     CDS             60310..61497
                     /gene="eutG"
                     /locus_tag="ACHOSM_RS00880"
                     /old_locus_tag="pgaptmp_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HGU4556781.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMVFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            61814..63040
                     /gene="eutH"
                     /locus_tag="ACHOSM_RS00885"
                     /old_locus_tag="pgaptmp_000177"
     CDS             61814..63040
                     /gene="eutH"
                     /locus_tag="ACHOSM_RS00885"
                     /old_locus_tag="pgaptmp_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HGU4556782.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            63037..64440
                     /gene="eutA"
                     /locus_tag="ACHOSM_RS00890"
                     /old_locus_tag="pgaptmp_000178"
     CDS             63037..64440
                     /gene="eutA"
                     /locus_tag="ACHOSM_RS00890"
                     /old_locus_tag="pgaptmp_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HGU4556783.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            64452..65813
                     /gene="eutB"
                     /locus_tag="ACHOSM_RS00895"
                     /old_locus_tag="pgaptmp_000179"
     CDS             64452..65813
                     /gene="eutB"
                     /locus_tag="ACHOSM_RS00895"
                     /old_locus_tag="pgaptmp_000179"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HGU4556784.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            65834..66696
                     /gene="eutC"
                     /locus_tag="ACHOSM_RS00900"
                     /old_locus_tag="pgaptmp_000180"
                     /pseudo
     CDS             65834..66696
                     /gene="eutC"
                     /locus_tag="ACHOSM_RS00900"
                     /old_locus_tag="pgaptmp_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
     gene            66706..67365
                     /gene="eutL"
                     /locus_tag="ACHOSM_RS00905"
                     /old_locus_tag="pgaptmp_000181"
     CDS             66706..67365
                     /gene="eutL"
                     /locus_tag="ACHOSM_RS00905"
                     /old_locus_tag="pgaptmp_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HGU4556785.1"
                     /translation="MPALDLIRPSITAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNLAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            67378..67878
                     /gene="eutK"
                     /locus_tag="ACHOSM_RS00910"
                     /old_locus_tag="pgaptmp_000182"
     CDS             67378..67878
                     /gene="eutK"
                     /locus_tag="ACHOSM_RS00910"
                     /old_locus_tag="pgaptmp_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HGU4556786.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            67924..68976
                     /gene="eutR"
                     /locus_tag="ACHOSM_RS00915"
                     /old_locus_tag="pgaptmp_000183"
     CDS             67924..68976
                     /gene="eutR"
                     /locus_tag="ACHOSM_RS00915"
                     /old_locus_tag="pgaptmp_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HGU4556787.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(68982..69881)
                     /gene="hemF"
                     /locus_tag="ACHOSM_RS00920"
                     /old_locus_tag="pgaptmp_000184"
     CDS             complement(68982..69881)
                     /gene="hemF"
                     /locus_tag="ACHOSM_RS00920"
                     /old_locus_tag="pgaptmp_000184"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HGU4556788.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(69885..70754)
                     /gene="amiA"
                     /locus_tag="ACHOSM_RS00925"
                     /old_locus_tag="pgaptmp_000185"
     CDS             complement(69885..70754)
                     /gene="amiA"
                     /locus_tag="ACHOSM_RS00925"
                     /old_locus_tag="pgaptmp_000185"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HGU4556789.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70968..71393
                     /gene="ypeA"
                     /locus_tag="ACHOSM_RS00930"
                     /old_locus_tag="pgaptmp_000186"
     CDS             70968..71393
                     /gene="ypeA"
                     /locus_tag="ACHOSM_RS00930"
                     /old_locus_tag="pgaptmp_000186"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGU4556790.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            71380..71829
                     /gene="yfeZ"
                     /locus_tag="ACHOSM_RS00935"
                     /old_locus_tag="pgaptmp_000187"
     CDS             71380..71829
                     /gene="yfeZ"
                     /locus_tag="ACHOSM_RS00935"
                     /old_locus_tag="pgaptmp_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HGU4556791.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            71890..72465
                     /gene="yfeY"
                     /locus_tag="ACHOSM_RS00940"
                     /old_locus_tag="pgaptmp_000188"
     CDS             71890..72465
                     /gene="yfeY"
                     /locus_tag="ACHOSM_RS00940"
                     /old_locus_tag="pgaptmp_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HGU4556792.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            72561..73460
                     /gene="yfeX"
                     /locus_tag="ACHOSM_RS00945"
                     /old_locus_tag="pgaptmp_000189"
     CDS             72561..73460
                     /gene="yfeX"
                     /locus_tag="ACHOSM_RS00945"
                     /old_locus_tag="pgaptmp_000189"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HGU4556793.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAVSKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(73518..74822)
                     /gene="pbp4b"
                     /locus_tag="ACHOSM_RS00950"
                     /old_locus_tag="pgaptmp_000190"
     CDS             complement(73518..74822)
                     /gene="pbp4b"
                     /locus_tag="ACHOSM_RS00950"
                     /old_locus_tag="pgaptmp_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HGU4556794.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(74825..76249)
                     /gene="murP"
                     /locus_tag="ACHOSM_RS00955"
                     /old_locus_tag="pgaptmp_000191"
     CDS             complement(74825..76249)
                     /gene="murP"
                     /locus_tag="ACHOSM_RS00955"
                     /old_locus_tag="pgaptmp_000191"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HGU4556795.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDASEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(76253..77149)
                     /gene="murQ"
                     /locus_tag="ACHOSM_RS00960"
                     /old_locus_tag="pgaptmp_000192"
     CDS             complement(76253..77149)
                     /gene="murQ"
                     /locus_tag="ACHOSM_RS00960"
                     /old_locus_tag="pgaptmp_000192"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HGU4556796.1"
                     /translation="MQLEKMITEGSNTTSAEIDRVSTLEMCRIINDEDQTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            77313..78170
                     /gene="murR"
                     /locus_tag="ACHOSM_RS00965"
                     /old_locus_tag="pgaptmp_000193"
     CDS             77313..78170
                     /gene="murR"
                     /locus_tag="ACHOSM_RS00965"
                     /old_locus_tag="pgaptmp_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HGU4556797.1"
                     /translation="MLYLTKISNAGSEFTENEQTIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            78299..79090
                     /gene="ucpA"
                     /locus_tag="ACHOSM_RS00970"
                     /old_locus_tag="pgaptmp_000194"
     CDS             78299..79090
                     /gene="ucpA"
                     /locus_tag="ACHOSM_RS00970"
                     /old_locus_tag="pgaptmp_000194"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HGU4556798.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            79261..80277
                     /gene="cysP"
                     /locus_tag="ACHOSM_RS00975"
                     /old_locus_tag="pgaptmp_000195"
     CDS             79261..80277
                     /gene="cysP"
                     /locus_tag="ACHOSM_RS00975"
                     /old_locus_tag="pgaptmp_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HGU4556799.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            80277..81110
                     /gene="cysT"
                     /locus_tag="ACHOSM_RS00980"
                     /old_locus_tag="pgaptmp_000196"
     CDS             80277..81110
                     /gene="cysT"
                     /locus_tag="ACHOSM_RS00980"
                     /old_locus_tag="pgaptmp_000196"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HGU4556800.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            81110..81985
                     /gene="cysW"
                     /locus_tag="ACHOSM_RS00985"
                     /old_locus_tag="pgaptmp_000197"
     CDS             81110..81985
                     /gene="cysW"
                     /locus_tag="ACHOSM_RS00985"
                     /old_locus_tag="pgaptmp_000197"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HGU4556801.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            81975..83072
                     /gene="cysA"
                     /locus_tag="ACHOSM_RS00990"
                     /old_locus_tag="pgaptmp_000198"
     CDS             81975..83072
                     /gene="cysA"
                     /locus_tag="ACHOSM_RS00990"
                     /old_locus_tag="pgaptmp_000198"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HGU4556802.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            83206..84117
                     /gene="cysM"
                     /locus_tag="ACHOSM_RS00995"
                     /old_locus_tag="pgaptmp_000199"
     CDS             83206..84117
                     /gene="cysM"
                     /locus_tag="ACHOSM_RS00995"
                     /old_locus_tag="pgaptmp_000199"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HGU4556803.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(84120..84488)
                     /gene="yfeK"
                     /locus_tag="ACHOSM_RS01000"
                     /old_locus_tag="pgaptmp_000200"
     CDS             complement(84120..84488)
                     /gene="yfeK"
                     /locus_tag="ACHOSM_RS01000"
                     /old_locus_tag="pgaptmp_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HGU4556804.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            84593..85444
                     /gene="pdxK"
                     /locus_tag="ACHOSM_RS01005"
                     /old_locus_tag="pgaptmp_000201"
                     /gene_synonym="thiJ"
     CDS             84593..85444
                     /gene="pdxK"
                     /locus_tag="ACHOSM_RS01005"
                     /old_locus_tag="pgaptmp_000201"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HGU4556805.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESNELI
                     LPPLAEA"
     gene            complement(85486..85995)
                     /gene="crr"
                     /locus_tag="ACHOSM_RS01010"
                     /old_locus_tag="pgaptmp_000202"
     CDS             complement(85486..85995)
                     /gene="crr"
                     /locus_tag="ACHOSM_RS01010"
                     /old_locus_tag="pgaptmp_000202"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGU4556806.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(86036..87763)
                     /gene="ptsI"
                     /locus_tag="ACHOSM_RS01015"
                     /old_locus_tag="pgaptmp_000203"
     CDS             complement(86036..87763)
                     /gene="ptsI"
                     /locus_tag="ACHOSM_RS01015"
                     /old_locus_tag="pgaptmp_000203"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HGU4556807.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(87808..88065)
                     /gene="ptsH"
                     /locus_tag="ACHOSM_RS01020"
                     /old_locus_tag="pgaptmp_000204"
     CDS             complement(87808..88065)
                     /gene="ptsH"
                     /locus_tag="ACHOSM_RS01020"
                     /old_locus_tag="pgaptmp_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HGU4556808.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(88449..89420)
                     /gene="cysK"
                     /locus_tag="ACHOSM_RS01025"
                     /old_locus_tag="pgaptmp_000205"
     CDS             complement(88449..89420)
                     /gene="cysK"
                     /locus_tag="ACHOSM_RS01025"
                     /old_locus_tag="pgaptmp_000205"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGU4556809.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(89605..90366)
                     /gene="cysZ"
                     /locus_tag="ACHOSM_RS01030"
                     /old_locus_tag="pgaptmp_000206"
     CDS             complement(89605..90366)
                     /gene="cysZ"
                     /locus_tag="ACHOSM_RS01030"
                     /old_locus_tag="pgaptmp_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HGU4556810.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            90596..91582
                     /gene="zipA"
                     /locus_tag="ACHOSM_RS01035"
                     /old_locus_tag="pgaptmp_000207"
     CDS             90596..91582
                     /gene="zipA"
                     /locus_tag="ACHOSM_RS01035"
                     /old_locus_tag="pgaptmp_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HGU4556811.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            91653..93668
                     /gene="ligA"
                     /locus_tag="ACHOSM_RS01040"
                     /old_locus_tag="pgaptmp_000208"
     CDS             91653..93668
                     /gene="ligA"
                     /locus_tag="ACHOSM_RS01040"
                     /old_locus_tag="pgaptmp_000208"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGU4556812.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPTRLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDWVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNVDEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            93670..93888
                     /gene="ypeB"
                     /locus_tag="ACHOSM_RS01045"
                     /old_locus_tag="pgaptmp_000209"
     CDS             93670..93888
                     /gene="ypeB"
                     /locus_tag="ACHOSM_RS01045"
                     /old_locus_tag="pgaptmp_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HGU4556813.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(93885..94883)
                     /gene="yfeH"
                     /locus_tag="ACHOSM_RS01050"
                     /old_locus_tag="pgaptmp_000210"
     CDS             complement(93885..94883)
                     /gene="yfeH"
                     /locus_tag="ACHOSM_RS01050"
                     /old_locus_tag="pgaptmp_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HGU4556814.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGLLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            94973..95899
                     /gene="yfeR"
                     /locus_tag="ACHOSM_RS01055"
                     /old_locus_tag="pgaptmp_000211"
     CDS             94973..95899
                     /gene="yfeR"
                     /locus_tag="ACHOSM_RS01055"
                     /old_locus_tag="pgaptmp_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4556815.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(95890..96231)
                     /locus_tag="ACHOSM_RS01060"
                     /old_locus_tag="pgaptmp_000212"
     CDS             complement(95890..96231)
                     /locus_tag="ACHOSM_RS01060"
                     /old_locus_tag="pgaptmp_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HGU4556816.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQCLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(96351..96426)
                     /locus_tag="ACHOSM_RS01065"
                     /old_locus_tag="pgaptmp_000213"
     tRNA            complement(96351..96426)
                     /locus_tag="ACHOSM_RS01065"
                     /old_locus_tag="pgaptmp_000213"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96391..96393),aa:Lys,seq:ttt)
     gene            complement(96431..96506)
                     /locus_tag="ACHOSM_RS01070"
                     /old_locus_tag="pgaptmp_000214"
     tRNA            complement(96431..96506)
                     /locus_tag="ACHOSM_RS01070"
                     /old_locus_tag="pgaptmp_000214"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96471..96473),aa:Val,seq:tac)
     gene            complement(96553..96628)
                     /locus_tag="ACHOSM_RS01075"
                     /old_locus_tag="pgaptmp_000215"
     tRNA            complement(96553..96628)
                     /locus_tag="ACHOSM_RS01075"
                     /old_locus_tag="pgaptmp_000215"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96593..96595),aa:Val,seq:tac)
     gene            complement(96673..96748)
                     /locus_tag="ACHOSM_RS01080"
                     /old_locus_tag="pgaptmp_000216"
     tRNA            complement(96673..96748)
                     /locus_tag="ACHOSM_RS01080"
                     /old_locus_tag="pgaptmp_000216"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96713..96715),aa:Val,seq:tac)
     gene            97007..98422
                     /gene="gltX"
                     /locus_tag="ACHOSM_RS01085"
                     /old_locus_tag="pgaptmp_000217"
     CDS             97007..98422
                     /gene="gltX"
                     /locus_tag="ACHOSM_RS01085"
                     /old_locus_tag="pgaptmp_000217"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGU4556817.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGR
                     TRSIERINNALAFIAERENQQ"
     gene            complement(98473..98865)
                     /gene="yfeD"
                     /locus_tag="ACHOSM_RS01090"
                     /old_locus_tag="pgaptmp_000218"
     CDS             complement(98473..98865)
                     /gene="yfeD"
                     /locus_tag="ACHOSM_RS01090"
                     /old_locus_tag="pgaptmp_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU4556818.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVYEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(98867..99226)
                     /gene="yfeC"
                     /locus_tag="ACHOSM_RS01095"
                     /old_locus_tag="pgaptmp_000219"
     CDS             complement(98867..99226)
                     /gene="yfeC"
                     /locus_tag="ACHOSM_RS01095"
                     /old_locus_tag="pgaptmp_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU4556819.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEEPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            99447..99522
                     /locus_tag="ACHOSM_RS01100"
                     /old_locus_tag="pgaptmp_000220"
     tRNA            99447..99522
                     /locus_tag="ACHOSM_RS01100"
                     /old_locus_tag="pgaptmp_000220"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99480..99482,aa:Ala,seq:ggc)
     gene            99731..101920
                     /gene="pdeA"
                     /locus_tag="ACHOSM_RS01105"
                     /old_locus_tag="pgaptmp_000221"
     CDS             99731..101920
                     /gene="pdeA"
                     /locus_tag="ACHOSM_RS01105"
                     /old_locus_tag="pgaptmp_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HGU4556820.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(101970..103172)
                     /gene="nupC"
                     /locus_tag="ACHOSM_RS01110"
                     /old_locus_tag="pgaptmp_000222"
     CDS             complement(101970..103172)
                     /gene="nupC"
                     /locus_tag="ACHOSM_RS01110"
                     /old_locus_tag="pgaptmp_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HGU4556821.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            103508..104746
                     /gene="mntH"
                     /locus_tag="ACHOSM_RS01115"
                     /old_locus_tag="pgaptmp_000223"
     CDS             103508..104746
                     /gene="mntH"
                     /locus_tag="ACHOSM_RS01115"
                     /old_locus_tag="pgaptmp_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HGU4556822.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(104887..105213)
                     /gene="ypeC"
                     /locus_tag="ACHOSM_RS01120"
                     /old_locus_tag="pgaptmp_000224"
     CDS             complement(104887..105213)
                     /gene="ypeC"
                     /locus_tag="ACHOSM_RS01120"
                     /old_locus_tag="pgaptmp_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HGU4556823.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(105328..106584)
                     /gene="yfeO"
                     /locus_tag="ACHOSM_RS01125"
                     /old_locus_tag="pgaptmp_000225"
     CDS             complement(105328..106584)
                     /gene="yfeO"
                     /locus_tag="ACHOSM_RS01125"
                     /old_locus_tag="pgaptmp_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HGU4556824.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASALQNLLWQR
                     LPGTLGIAQDSPFWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            106788..107753
                     /gene="glk"
                     /locus_tag="ACHOSM_RS01130"
                     /old_locus_tag="pgaptmp_000226"
     CDS             106788..107753
                     /gene="glk"
                     /locus_tag="ACHOSM_RS01130"
                     /old_locus_tag="pgaptmp_000226"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HGU4556825.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            107973..108299
                     /gene="fryB"
                     /locus_tag="ACHOSM_RS01135"
                     /old_locus_tag="pgaptmp_000227"
     CDS             107973..108299
                     /gene="fryB"
                     /locus_tag="ACHOSM_RS01135"
                     /old_locus_tag="pgaptmp_000227"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGU4556826.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            108321..109568
                     /gene="fryC"
                     /locus_tag="ACHOSM_RS01140"
                     /old_locus_tag="pgaptmp_000228"
     CDS             108321..109568
                     /gene="fryC"
                     /locus_tag="ACHOSM_RS01140"
                     /old_locus_tag="pgaptmp_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGU4556827.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLVFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            109583..110668
                     /gene="ypdF"
                     /locus_tag="ACHOSM_RS01145"
                     /old_locus_tag="pgaptmp_000229"
     CDS             109583..110668
                     /gene="ypdF"
                     /locus_tag="ACHOSM_RS01145"
                     /old_locus_tag="pgaptmp_000229"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4556828.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            110668..111705
                     /gene="ypdE"
                     /locus_tag="ACHOSM_RS01150"
                     /old_locus_tag="pgaptmp_000230"
     CDS             110668..111705
                     /gene="ypdE"
                     /locus_tag="ACHOSM_RS01150"
                     /old_locus_tag="pgaptmp_000230"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4556829.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDWQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLYTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            111730..114225
                     /gene="ptsP"
                     /locus_tag="ACHOSM_RS01155"
                     /old_locus_tag="pgaptmp_000231"
     CDS             111730..114225
                     /gene="ptsP"
                     /locus_tag="ACHOSM_RS01155"
                     /old_locus_tag="pgaptmp_000231"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4556830.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(114228..115085)
                     /gene="ypdC"
                     /locus_tag="ACHOSM_RS01160"
                     /old_locus_tag="pgaptmp_000232"
     CDS             complement(114228..115085)
                     /gene="ypdC"
                     /locus_tag="ACHOSM_RS01160"
                     /old_locus_tag="pgaptmp_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4556831.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKQVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(115098..115832)
                     /gene="ypdB"
                     /locus_tag="ACHOSM_RS01165"
                     /old_locus_tag="pgaptmp_000233"
     CDS             complement(115098..115832)
                     /gene="ypdB"
                     /locus_tag="ACHOSM_RS01165"
                     /old_locus_tag="pgaptmp_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HGU4556832.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(115847..117544)
                     /gene="ypdA"
                     /locus_tag="ACHOSM_RS01170"
                     /old_locus_tag="pgaptmp_000234"
     CDS             complement(115847..117544)
                     /gene="ypdA"
                     /locus_tag="ACHOSM_RS01170"
                     /old_locus_tag="pgaptmp_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HGU4556833.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGMGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPYFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPDNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT        28059 a        30083 c        31801 g        27819 t
ORIGIN      
        1 tattttgttt gatatatatc aattggaact ttcatatgac acttagaatc aatgctctct
       61 atagtgatga ataatcatca ttcgaagtca ggtgggatgc ctgtctgaat acacctcctt
      121 caggatgtgg gggattctgc tgagcatcta tgaaactgaa tgcaacttat ataaaaatac
      181 gtgataaatg gtgggggctt ccgctgttct tgccttcttt aatcttgccc attttcgccc
      241 acattaatac tttcgcgcat atttcttccg gtgaggtttt tctcttttat ctgcctctgg
      301 cactgatgat cagcatgatg atgtttttca gctgggcggc attgccaggg atcgccttag
      361 ggatttttgt ccgcaaatat gcagagctgg gtttttacga aacgctatca ttaacggcta
      421 attttattat cattatcatt ctctgttggg gcggttacag ggtctttact ccccggcgta
      481 acaacgtttc acatggtgat acccgtttaa tttcccagcg tatattctgg cagatagtgt
      541 ttcctgcaac gctgtttctg atacttttcc agtttgctgc atttgtagga ttactggcga
      601 gcagagaaaa tctggtcggt gtcatgcctt ttaacctcgg gactttaatc aattatcagg
      661 ccttactggt gggtaatctg atcggtgtcc cgctgtgcta cttcatcatt cgggtagtgc
      721 gaaatccatt ttatttacgt agctattatt cgcaattaaa acagcaggtt gatgccaaag
      781 tcaccaaaaa agagttcgcg ctctggctac tggcattagg tgctttattg ttgctgttat
      841 gcatgccgtt aaatgaaaaa agcacaattt ttagcaccaa ttataccttg tcattattgt
      901 tgcccctgat gatgtgggga gcaatgcgct atggttataa gctgatttcg ctgctctggg
      961 cggtcgtgtt gatgatcagc atccacagct atcaaaatta cattcccatt tatcctggct
     1021 ataccacgca gctgaccata acctcctcca gttatctggt attctctttt attgtcaatt
     1081 atatggctgt actggcaact cgtcagcgag cggtagtcag acgcattcag cggcttgcat
     1141 atgtggaccc ggtggttcat ctgccaaatg ttcgcgccct gaatcgcgcg ttgcgtgatg
     1201 ccccctggtc tgcgctttgt tatttacgca tccctggcat ggaaatgctg gttaagaact
     1261 atggcatcat gctgcggatt caatacaagc aaaaactttc tcactggctg tcacccttgc
     1321 tggaaccggg tgaagatgtt tatcagcttt cgggtaacga tctcgcgctg cggctgaata
     1381 cagaatcgca ccaggagcgc attaccgcac tggatagcca tctcaagcaa tttcgtttct
     1441 tttgggatgg catgccgatg caaccgcaga ttggcgtcag ttactgctat gtgcgctcgc
     1501 cagtgaatca tatctacctg ctgctgggag agctaaatac ggtcgccgaa ctttccatcg
     1561 tgaccaacgc cccggaaaat atgcagcgtc gcggggtaat gtatttgcaa cgcgaattga
     1621 aagataaagt cgcgatgatg aatcgactac agcaggcgct ggaacacaac cattttttcc
     1681 tgatggccca gccgattacc ggtatgcgtg gtgatgttta ccatgaaatt cttctgcgca
     1741 tgaaaggtga gaatgatgaa ctgatcagcc ccgatagctt cttgccggtc gcgcacgaat
     1801 ttggtttatc gtcgagtatc gacatgtggg tcattgagca tacgctgcaa tttatggctg
     1861 aaaacagagc gaagatgccc gctcaccgtt ttgctattaa tctgtctcca acctcggtat
     1921 gtcaggctcg ttttcctgtt gaagtcagtc agttgctggc taaatatcag attgaagcgt
     1981 ggcaacttat ttttgaagtc accgaaagta atgctctgac caatgttaag caggcgcaaa
     2041 tcaccttgca gcatcttcag gaattaggct gccagattgc gattgatgat ttcggcaccg
     2101 gctacgccag ctatgcacgg cttaaaaatg tgaatgccga tctgcttaaa atggacggca
     2161 gctttatccg caatattgtg tcaaatagtc tggattatca gatagtggcg tcgatttgcc
     2221 acctggcgcg aatgaagaaa atgctggtag tggcagagta cgttgaaaac gaagagatcc
     2281 gcgaggcagt gctctctttg gggatcgatt atatgcaggg ttatcttatt ggtaagccgc
     2341 aaccgttaat tgatacgctg aatgaaatcg aacccattcg tgaaagtgcc tgaataatgc
     2401 gggccgacat ttctcgtcgg cccgcaaagc attaagcggc gatttcaggt gtactttctt
     2461 cttcaatttt caaccgccag ccagccacgc cttcccagta ttcttgctcc ttttccagat
     2521 caagcagtac cagcgcattc tgactaaacc agtcatgcgg gaaacgcagt gtccagtgac
     2581 tgtcatcagt aatcagtgtc aatgttggcg gtgtggtggt tgcctgacgt tgattgttta
     2641 gtaatacgcc aaggcgcaat agctgtatca gtggcaggaa ctgtttcttc ttaaacaagg
     2701 taaagcgcgg cagatcgtcg agcttaatcg ctttacggtg atagcgcacc agtgtcgcca
     2761 tcatcagctg ctgttcctga ttaaaacccg gcaagtcact gttttgcaga atataagcgg
     2821 agtggcgatg caaaccgctg tggttgatat tcaacccgac ctcatgcagc atggcggccc
     2881 atcgcagtag cgcctccagt tgcggatgcg ccagcttcgg ttgctgttcc cgccactgtt
     2941 cgtacatttg catagtggta tccagcaccc gtcgggcctg ctcgctgtcg atgtgatact
     3001 ggttggcgag gctgctggcg gtgcgactac gcacatcctg atgacggaaa cgtccttcca
     3061 tttcatacag tacgccttcg cgtaaagccc cgtcagaaag gcgcagttca cggatggcta
     3121 aagcatcaaa cacaccgcat aaaatcgcca gtccaggaac gaagactgtt ttccgctctt
     3181 cggaaagacc cggtaaactc agcgatgcga aattacggtg acgtaaaact tcttttacca
     3241 gtttttccag acgttccggg gtaattatcc cgtctttctc gcccatttcc attaacactt
     3301 catgggcggc ttttatggta ccggaagcgc ccattgccac gttccagccc tgaatacgga
     3361 attgccaggt taaagtttcc agtttttgtg ctgccgccat acgagcgcgc tgaaaattct
     3421 ctttattgat gaccccgcca gggaagtaaa gctgggcaaa gctgacacaa cccatccggc
     3481 ggctttcaac gagaatcggt tcgaaatttt caccaatcac cagttccgta gatccgccgc
     3541 caatatcaat aaccagtttg cgacctttct ccggttgggt atgttccacg cccataaaaa
     3601 tcagacgggc ttcttcatta ccggaaataa tttcaatcgg gtagggaatg accttttccg
     3661 cgcgtttcag aaagtcagtg gcgttcagcg cctgacgcag cgtatgggtg ccgactatac
     3721 agacgctggc aggagaaaac ccttgtagcc gttcggcaaa cagcgacaga cagttcaaac
     3781 cgcgcgtcat tgcctcttca ctcaacatat tatctggccc caggccgtcc gccagatgca
     3841 cccgctgttt caggcggcca ataatctgca tggcaccatc taccacacgg gctatgacca
     3901 tgtgaaaact gtttgaacca agatcgaccg cagcaaactc ctgcggacga ggggatttat
     3961 cgtgtattgg catagggtta ttcaggttgt tcgagtgatt tgatgtagtc gtaaatcgcc
     4021 aactgcgccc gtactttgcg gcgattgctg cggggaacgt agcgattact gagttcttta
     4081 tcgatataac gtgctttgac cgtatcgctg aacaatatgt cgatgacgtc cagcacccgc
     4141 tgcttcaggc gcggatcgag cagcggcgtc gccacttcaa tacgataatc aatattgcgc
     4201 gtcatccagt cggcggaaga aaggtagacc tttttatcgc cgccattttc aaaaatataa
     4261 acccggtcat gttcaaggta acggtcaaca atactgatgg cacgaatgtt gtcgctaatg
     4321 ccttccagat tggggattag cgaacacatt ccgcgaacca gcagattaac cggtacgccg
     4381 gagctggagg ccgcatacag acgatcaacc aggcctttat cgacaagatt atttagcttc
     4441 agggtgatac cgctgggcag cccttgctgc gcgttggcga tctcgcggtc caccatttca
     4501 tacaacaggc ggcgggagtt ttgcggcgac accattaaat aatcaaatgt cactgggcgg
     4561 tatgggtttt caataaagtt aaatacccgc cgtacttcgt tggtgatacg cgcatctgcg
     4621 gtcagcaacg aatagtcagt ataaagacgc gcggtttttt cgttaaagtt cccggtcccg
     4681 atgtgtgcgt aacggaccac ttcaccgttt tctttacgtg aaatcaggaa cagtttggcg
     4741 tgaattttca gccccggcgc agagaagata acgtgcacgc ctgcttcggt caggcgcttc
     4801 gcccagtgaa tgttggcttc ttcgtcgaaa cgcgcctgta actcaaccac cacggtgact
     4861 ttcttaccgt tgtgtgcggc gtggatcatc gagtcgatga tgcgtgaatc tttcgccacg
     4921 cggtaaatgt taattttaat cgccagtacg ctcgggtcga acgaagcctg acgcagcagt
     4981 tccagcacat gctcaaaggt gtgataagga taatagagca acacatcgcg ttcgcgaatg
     5041 gcatcaaaac cattgcggaa ctgggcttta tcaaaccaaa tatggcgtaa acgcggcagt
     5101 ggtttgttca ccagattggc tttgccgaca ttggggaaat taataaagtc tttaaaatta
     5161 tgataacgac cgccggggac gatggagtcg tagcgggaaa tagtcagttt ttcgcgtaac
     5221 acttcaacca gcgcattggg catatcgcgc tgataaacaa aacgcaccgg ctcagcagtt
     5281 aaacgctgct tgagactgga agacatcaac tccatcaggc tggcttccat ctcatgcact
     5341 aaatcgtatt cggcatcgcg ggtcatcttc attgaatagg cattcagcgc gtcataatca
     5401 aagaagcctt tgaaaatatc atcaaggcag taacgcagaa tgttatccag aagaatcatc
     5461 ggcttgcgtc gacgcggcgc ttctggcggt aaattcacaa agcgcggcac tttatctgat
     5521 gggatctcca gcagcgcgta acggatggta tcaccacgga taatttccac cgccagatag
     5581 gtgtaatcat ccttcaggaa ctgcactaag tcagtgtcag gattgattaa aatcggcgta
     5641 atgtgctgac gcagatactg cttaaaataa tgacgaagcc agttttgttg attgacggag
     5701 agctggcgtt cattaatcag gaagatctgg ttgcgcgcca tctccagcaa tagctcgttg
     5761 tagaggccgt cgaattcctg atcggctttc agcacccggg actgaatttt gcccagtaaa
     5821 tggcgggaat gagagttgga gccttgttct tcgctaataa tgatgcgtcg cttcagttca
     5881 gcgaagcgga ctttatagaa ctcatcaagg ttattggaat agatccccag gaaacgcatc
     5941 ctttcaatca gcgggttaga tttgtccgcc gcttcctgaa gcacgcgttc attgaacgat
     6001 aaccaactga gctctttttc gatgtatagc ttttcctgac ccattaccac ttttactccg
     6061 ttttattcac gggacactgc ttggatatta tggcgagcaa tattggcaga tgtccaacta
     6121 tgccagaaaa gggggaaaaa aactgctgga gcttgcgatt tacaggtgaa ttgaaaaacg
     6181 ccagcggcag agctggcgct ttaactacgg gggcattact cgtcggcagc gtagccctgc
     6241 ggcggcagac gttgaccatc cagccacgcg gcgttttcgt gcattttcag acgaccatcg
     6301 gcaaaccagc taatcaccag tggataaata gcgtgttcct gggtttgcac gcgggcggtg
     6361 acgtcatctt ccgtatcacc agcaaatacc gggactttcg cctgtaaaat aaccgggcca
     6421 ccgtccagtt catcggtgac gaaatgcacc gatgtaccgt gctcttcatc accattttcc
     6481 agcgcctgac gatgggtgtg taatccggga tatttcggca gcagagaagg gtgaatgttc
     6541 agcaaacgcc cggcatagtg ggagacaaac gccgggctga gaatgcgcat aaaaccagcc
     6601 agcacgacca catcgggtgc gtacatgtcg atttcatgaa tcaactcccg gtcataggct
     6661 tcacgactgt caaacgcgct ggcgatgagc gtatgcgttg caatacccgc ctggcgggcg
     6721 cgttcaaggc cgaacgcgtc ggccttattg ctgaaaactg cccgtacggt gcctttaatt
     6781 ttgttggttt tacaggcgtc aataattgcc tgtaaattac ttccgttgcc ggaaataagc
     6841 accacaatat tcattattcg ataaccacgc gttgttcgga atcagaggct ttgatgatac
     6901 cgattttcca cgcgttttta ccgttggcat ttagcagggc gagggctttg tccacttccg
     6961 gagcaggcag ggcgataatc atcccgacgc cgcagttgaa ggtgcgatac atttcatggc
     7021 gctcaacgtt accagccgtt tgcagccagt tgaacacttc cggccactgc caggaagatt
     7081 catcaatcac tgcctgagta ttatctggca atacgcgtgg aatgttttcc cagaagccgc
     7141 cgccggtcag gtgcgcaatg gcatgcacat cgaccttttc aatcaactcc agcactgact
     7201 tcacgtaaat gcgggtcggt gccagcagat gatcggctaa tggcttacca tcaagttcgg
     7261 tggtttgcgg atcacaaccg ctgacttcaa gaattttgcg caccagcgaa tagccgttcg
     7321 agtgcggacc gctggaaccg agtgcaatca gcacatcgcc gtcgctgact ttagagccgt
     7381 cgatgatttc tgatttttct accacgccaa cgcagaaacc cgcgacatcg taatcctcac
     7441 cgtgatacat ccccggcatt tctgccgttt cgccacccac cagtgaacag cccgattgca
     7501 gacatccttc cgcgataccg ctgatcaccg ctgaagcggt atcaacatcc agttttccgg
     7561 ttgcgtaata gtcgaggaaa aacagcggct ctgcaccttg caccaccagg tcattaacgc
     7621 acatggcgac cagatcaata ccaatggtgt cgtgacgttt taagtccatt gccagacgca
     7681 gcttggtacc tacgccgtca gtgccggaaa ccagcacggg ttcacgatat ttttgcggca
     7741 atgcacacag cgcaccgaag ccgcccagac cgcccatcac ttccggacga cgcgttttct
     7801 tcactacgcc tttgattctt ccaaccagag cattacccgc gtcaatatca acaccggcat
     7861 ctttgtagct aagagaggtt ttatcggtca ctgcttgggt ccccacgcgt tacttgcggt
     7921 agaaaaataa aattcggcgc aattctaaca gggaaagcaa acgtttgcga gactgcttta
     7981 cacaaccttt ttgcacgtct tttgcccagg cgcgcggcga aagaagactt gtaccagggt
     8041 aaaggttagt tttcggatgg aataatcttc tttcataacc atttgaatat aaaataactt
     8101 tatctcaaac cgttatcatt ttgactaaag tcaacgaaaa gaatattgct accttgaaga
     8161 aaggaggtat aatccgtcga tttttttgtg gctgcccctc aaaggagaaa gagtatgaag
     8221 atcgtggaag tcaaacaccc actcgtcaaa cacaagctgg gactgatgcg tgagcaagat
     8281 atcagcacca agcgctttcg cgaactcgct tccgaagtgg gtagcctgct gacttacgaa
     8341 gcgaccgccg acctcgaaac ggaaaaagta actatcgaag gctggaacgg cccggtagaa
     8401 atcgaccaga tcaaaggtaa gaaaattacc gttgtgccaa ttctgcgtgc gggtcttggt
     8461 atgatggacg gtgtgctgga aaacgttccg agcgcgcgca tcagcgttgt cggtatgtac
     8521 cgtaatgaag aaacgctgga gccggtaccg tacttccaga aactggtttc taacatcgat
     8581 gagcgtatgg cgctgatcgt tgacccaatg ctggcaaccg gtggttccgt tatcgcgacc
     8641 atcgacctgc tgaaaaaagc gggctgcagc agcatcaaag ttctggtgct ggtcgctgcg
     8701 ccagaaggta tcgctgcgct ggaaaaagcg cacccggacg tcgaactgta taccgcatcg
     8761 attgatcagg gactgaacga gcacggatac attattccgg gcctcggcga tgccggtgac
     8821 aaaatctttg gtacgaaata aagaataaaa ataattaaag ccgactttaa gagtcggctt
     8881 ttttttgagt aaagcgccta taacacataa tacagaggat aatactatga cgcgccgtgc
     8941 tatcggggtg agtgaaagac cgccactttt acagacaatc ccgcttagtt tgcaacattt
     9001 gttcgccatg tttggtgcaa ccgtcctggt gcccgtctta tttcatatta acccggcgac
     9061 tgtactgtta tttaacggta ttggaacgct gctgtatctc ttcatctgta aagggaaaat
     9121 tccggcttat cttggttcca gctttgcctt tatttcaccg gtattgttac tgttgccgtt
     9181 agggtatgaa gtcgcgctgg gcggctttat tatgtgcggc gtgctgttct gcctggtttc
     9241 ttttatcgtg aagaaagcgg ggaccggctg gctggacgtg ctgtttccac ctgcggcaat
     9301 gggcgcaatc gttgccgtca tcggtctgga gctggcgggc gtagctgccg gtatggcggg
     9361 tttactcccg gctgaagggc aaacgccaga ctccaaaacc atcatcatct ctattaccac
     9421 cctggcggtc acggttttag gttccgtgct gtttcgtggt ttcctggcaa ttatcccgat
     9481 tttaattggc gtgctggtgg ggtacgcgct ctctttcgca atgggaattg tcgataccac
     9541 gccgattatt aatgctcact ggtttgcgct gccaaccctc tatacgccgc gcttcgagtg
     9601 gtttgccatt ctgactattc tgccagcggc gttagtggtt attgccgaac acgtagggca
     9661 cctggtagta acggctaata tcgtcaaaaa agatctgctg cgcgatccag gtctgcaccg
     9721 ttcgatgttt gctaatggct tgtcgaccgt gatttccggc ttctttggct ctacgccaaa
     9781 taccacttac ggagaaaaca ttggcgtgat ggcgatcacc cgtgtttaca gtacctgggt
     9841 tatcggcggg gcggcgattt tcgctatcct gctttcctgc gtcggtaaac tggctgccgc
     9901 tatccagatg atcccattgc cggtgatggg cggcgtttcg ctgctgcttt atggtgtcat
     9961 cggtgcttcc ggtattcgtg ttttgatcga atcgaaagtg gattacaaca aagcacagaa
    10021 cctgatcctg acttccgtga ttttgatcat cggcgtcagt ggcgcgaagg taaacatcgg
    10081 cgcggcggag ttaaaaggta tggcgctggc gaccatcgtc ggtatcggct taagtttgat
    10141 tttcaaactg atcagtgtgc tgcgccctga agaagtggtg ctggacgcag aagacgcgga
    10201 tataacagac aaataatgta ccgaccgggc agtgttgctg cccggttcaa acatcatggg
    10261 attctgtggt aaacttctcg cgattttgtg aaatcctggt tgaggtatct ctgaacacac
    10321 cggcacagct ctctttgcca ctttatcttc ctgacgacga aacctttgca agtttctggc
    10381 cgggggataa ctcctcttta ctggccgcgc tgcaaaacgt gctgcgtcag gaacatagcg
    10441 gttacatcta tctctgggca cgcgaaggcg cggggcgcag ccatctgctg cacgcggctt
    10501 gcgcggaatt gtcgcagcgt ggcgatgcgg tgggctatgt cccgctggat aaacgcacct
    10561 ggtttgttcc ggaagtgctc gacggtatgg agcatttgtc gctggtctgt atcgacaaca
    10621 ttgagtgtat tgcaggcgat gagttgtggg agatggcgat tttcgatctc tacaatcgaa
    10681 ttctggaatc gggcaaaaca cggttgttga tcaccggcga tcgtccaccg cggcagttga
    10741 atctgggatt accggatctc gcgtcgcgac tcgactgggg gcagatctac aaattgcagc
    10801 cactttctga tgaagataag ttgcaggcgc tacagttacg cgcgcgtttg cgtggttttg
    10861 aactgccgga agatgtgggg cgtttcttgc tgaagcggct cgacagagaa atgcgcacgc
    10921 tatttatgac gttggatcag ttggatcgtg cgtcgattac cgcgcaacgt aagctgacca
    10981 ttccgtttgt gaaagaaatt ctgaagttgt agataaggtg tttattgtcg gatgcggcgc
    11041 gaacgcctta tccgaactac gcgaagttaa tccatagtat tcgtaggccg gataagacgt
    11101 ggcaagcgtc gcatccggca atgtgcttca actagcgatg gagcacatct taacccacga
    11161 tttccagtac ctgctccggt ggacgaccaa tccgcgcttt gccattcgcg accacaatcg
    11221 ggcgctccat tagcttcgga ttatccacca ttgcctgaat cagcgcctct tcgctaagcg
    11281 aactgtcagc caggttcagt tctttataaa gatcctcttt ctggcgcatc aattcacggg
    11341 cgctattcat ccccagcatt ttcagcaaat cgcgcagcgt tgccgcatcg gcgggtgtct
    11401 caaggtagag caccacttct ggctccacgc cgttttcttt cagcaggttc agcgtttccc
    11461 ggctctttga acagcgtggg ttatggtaga tttttacctg tttggtcatt gttcttcctt
    11521 taatgcgaat tacatcttgg tataaggctt aaagcgttcc tgcagctggc gcaactggtc
    11581 gatgcgcgca tcgtaacgcg cttgttgcag gctgcctaat ttcacctgcg aactggcgct
    11641 actcaacagc gaaatggcct gatcgagtcg cccggcgagc gcataacctt ctgctcgcgc
    11701 agccagttcc tgatcgcggt tatttagcgc ggcttccgcc tgtgccagta aatcccagcc
    11761 gttgctgtca tctttattgt taaaagtgta gcgattgaga atattggccg cttcttgtgg
    11821 ttgaccgcct tgtagataag cgttcgccag gttgagctgc aacaccggat tggtgcgcaa
    11881 atcacgggca tttttcaggc gattgattgc ctcattggct ttgttttgcc caagatcgat
    11941 atcggtagcc agatcgagat accatgcgtt gccaggttct gccgccagta acggttgcag
    12001 cgttttacgc gcctcgtcgt atttattggc ttccatcgcc tgtaaagcac gaccatattg
    12061 cgcagctcgt tgctgacgaa cgtttccttt cgcccattca tccagcaaat cactggtgag
    12121 ctggttacgt ccggaattat acatccccag tgtgcgcgct tttgccagat agaaatcttc
    12181 cgacgactgc accaccatcg ggcgcatctg attagcacgg ttgcgggcat ctgccagacg
    12241 actttccggc aacgggtgag tcaataaaat ttccggcgga cgcgaggagt aacgcgcctg
    12301 atcgagtaat ttttcgagga aggtcggcat cgcctgcgga tcgaatcctg agcgttgcag
    12361 cacctgaata ccaatgcggt ccgcttcctg ttcattttgc tgggtgaaac tgatcatccc
    12421 ctgacgcgtt cccgccagtg taccggtcag cgccgccatc cccgcctgcg gactggccat
    12481 cgccagtaaa atagaaccta acgcgccgac ccaggtcagc ggcgcgctgc gctgctgatc
    12541 ttccatcgct cgcgccaggt gacgttgggt gacgtgggag atttcgtgcg ccataactga
    12601 agccagttga ctttcgttat cggaataacg gaacagggca gagtgcagca ccacgttgcc
    12661 gccaaagaaa gcaaaggcgt taatttcgtc gttgttgatc agaaaaaaat gaaacggtgt
    12721 cttaaccgaa ttggcatgcg aaaccagacg catccccagc gaattaatat attgcgttaa
    12781 cagcgggtca ttaattaacg gcgcgctgcc gcgtagctgg cggacataat agtcgcccat
    12841 ctgcatttcc tgaccaatgg aaagcgtgct tcctgcggag gttcccatat ccggcaaggt
    12901 gtctgcgctg tcggcaaacg ccggggctac ctgaccaatg gtcatagcag caatgagggt
    12961 tgcaaccagg ttttttttca actgcctgaa cataacctct atcctgtatt tctgaagata
    13021 gtcatttgac cgatactgtt aaatatcgtt cccaccgcgc cgcctttgag tgtaacggcg
    13081 aagattagcc cactcaaatg acttttcagt ttcctgaatc cgtcataaaa agcgaagtct
    13141 tttttgtgac atttcgatac aattgcggat cgcaatcccg ctcttgaggt tcagggaagg
    13201 tttttatgct cgaaatgttg atgcaatggt atcgccgccg ttttagcgac ccggaagcga
    13261 ttgccttgct ggttatttta gttgccggat ttggcattat ctttttcttt agtggcctgc
    13321 ttgctccgtt gctggtggct attgtgctgg cctatttgct ggaatggcca accgtgcgcc
    13381 tgcaatctat tggctgctcc cgccgctggg cgacgtcgat tgtattggtg gtttttgtcg
    13441 gtatattgct actgatggcg ttcgtggtac tgcctatcgc ctggcaacag ggcatctact
    13501 taatccgcga tatgccgggg atgctcaata agctttctga ctttgccgcc acgttgccgc
    13561 gccgctatcc ggcgttaatg gacgcgggca ttattgatgc aatggccgaa aatatgcgca
    13621 gtcggatgct gaccatgggc gattcggtgg tgaaaatttc cctcgcctcg ctggtcggtt
    13681 tgctgaccat tgccgtctat ctggtgctgg tgccattgat ggtcttcttc ctgctgaaag
    13741 acaaagagca gatgctgaac gccgttcgtc gggtgctgcc gcgcaaccgt ggactggcag
    13801 gacaggtgtg gaaggagatg aatcaacaaa tcaccaacta tatccgcggc aaagtgctgg
    13861 agatgatcgg tggtggggat cgccacctgg ctggggttct tgctctttgg gctgaactat
    13921 tcgctgctgc tggcggtgct ggtcggcttc tcggttctta ttccgtacat tggcgcattt
    13981 gtggtgacca ttccggtggt tggcgtggcg ctattccagt ttggcgcagg cacggaattc
    14041 tggagctgct tcgcggtgta tctgattatt caggcgctgg acggcaacct gctagtaccg
    14101 gtgttgttct ccgaagcggt taacctgcat ccgctggtga ttattttatc ggtggtgatc
    14161 ttcggtggtt tgtggggatt ctggggcgta ttcttcgcca ttccattggc gacgctgatc
    14221 aaagccgtga tccacgcctg gcccgatggg caaatcgcgc aagaataatc ttcagcccga
    14281 cgaactgttt cgccgggctg atttttcagg attcctgacg taaataaata gcccgataac
    14341 aaatactcac cagcactgca ccgccgataa tattacccag catcaccggg agcagattgg
    14401 cggtaataaa atggctgacc gtcagtgccg gaaaattgtc tgcgctactg tgcgccagtt
    14461 gccagaagga ggtaggggcg aaatggcgaa tggcaatggc gaacggaatc acaaacaaat
    14521 tggcgataca gtgctcaaag ccactggcga caaacagggt gatgggcaaa atcatggcga
    14581 cgattttgtc gcataacgaa cgcccgcaat aactcatcca cagcgccagg caaaccatca
    14641 gattgcacat aatgccgagg ctgacagatt cagtaaatgt atgatgcatt ttgccctcgg
    14701 cgcagtgtaa aaccgcgact ccccacatcg cgttttcact catcaccagc ccggaaaacc
    14761 agattaacaa actgaaacag gcaatacctg ccagattacc acaggccacc agaagtgcgt
    14821 taatcagcca agttcgccaa ctaataacgc cccgactttt tgccattacc gtcattaccg
    14881 acgaggtgaa caggttggtg ccgcaaaccg ccagcaaaat aaagccgagt gtaaagcaaa
    14941 ggccgcccac cagatgggtt aatgcctgtg aagacggggc atcggcgata acggaaaggt
    15001 aaaaagtaaa tccaatcgcc ataaacacgc cagcggacat cgccattaaa aagaccgaca
    15061 ctttgctgcg ggcaatttta tgggcagcaa tccgttcagc gattgccgct tttctggcgc
    15121 tcaactgcaa gtcgaaagag agtttgtttc tcatgcatac tcctgcaaaa gcagattaca
    15181 acacctcgcg aaccgagatc cccagacgct gcattcgtga cagcagcgtg gtgcgcttca
    15241 tccctaatcg tgtcgccgcg ccacggggcc cggcaacaat gccattggtt tctcgcaata
    15301 cctgaacaat gcgctgacgt tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact
    15361 gcgccattga actgcgaagc gctgaatctt catttagcgt cggcagtaaa cggctttgtc
    15421 ggacattaag atgtaaattc agactgttac cgcgagtcag cagtaccgct cgctcaatca
    15481 cgttttccag ctcgcgcacg ttgcccggcc agtcccacga catcaactgg cgtagcgcct
    15541 cggtcgggat ggcgtcaatt gaacgattca tatggcgcgc cattttttgc gtgaaatgct
    15601 ttgctaaaag agggatatct tccggacggt cgcgtagcgg cggcaattcc agtgggaaga
    15661 cattcaggcg ataaaagaga tcgctgcgaa actggcgatc ttcaaccatt tgccacaaat
    15721 cacggttggt ggcggcaatg actctgacat tcaccgggat cgttctactc ccgccgagac
    15781 gctcaatctc ccgttcctgc aatacgcgca gcagtttagg ctgaagttct aacggcagat
    15841 cgccaatttc atcgagaaac aacgtgccgc catcggcaat ttcaaaacga ccacgatggg
    15901 tattaatcgc accagtaaac gcccctttgt catgaccgaa taactcactt tccagaagac
    15961 tggcggggat ggcagcacag ttgattttga ccagcggctt gtcgtgtcgc gggctaagtt
    16021 gatggatcgc tctggcgatc acctatttgc cggttccggt ttcaccacaa atcaacaccg
    16081 tactgtcgct cttcgccaca atatctacct gctggagtag gtcttccatg gcctggcttt
    16141 gatagataat gtcaccgatg cccagattcg aaaggagctg ctcgctaagc tggtggtttt
    16201 cttgctgcaa actttcctgc aaatcggtca tgctacgcca ggcatcggca ttgccaacgg
    16261 caatagcgat gcgatcggct atgtgttgta gtagctggca gttttcctca ctaaagagag
    16321 tggaagaggt atgcgccagc aacaatgcac ccggtgtatg gttgccaaag gtaagcggta
    16381 taaggagcgc agattcgcag ccgttagatg caagtaagag taataacggg tcgcgttgcc
    16441 agagaaacag atcgtctgca cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg
    16501 tcagcaatac actgtcgcca ggcatattgc gtggcagaca cgcacaatgt gaggcagaaa
    16561 gatcgctgca ccacaggcta aacttctcgt tctttcgatg atcgcccagt accatactga
    16621 ctgaagccag accgaaaaaa tgatggatct cacgagcgac gtcagcgatc agatcgtcga
    16681 gatcaagatg tgacagcact gcattagtaa tatcaaccag tacccgatga tgatcgcgat
    16741 ctttctccag caaacgcagc gccacctgtt gttcttcatc attgagccat gattgcagaa
    16801 ccggagtgat aatgcgtgcc agccagttaa acagtgtgca atcctgggga gtaaaacggc
    16861 tgccgtgggt acgaataaag cgtagctccg ccattccact actgccgaga aggacggcgt
    16921 gacgcgaata atcgaccatc tcgccctgat actctgttgt tgccgtcaca tcgctgccat
    16981 catcatggta gcggtaatag aagttgcttt tgagaacatg cagttcaata agttgtaccg
    17041 ggctgaacgg cggcggcatc gggatgaagg cgcgtaataa agacgcctta ccgtgtttct
    17101 gtgctaaccg ctccgcgagc attccgttta ccgcttcaat tgttattcct tgtggcgggg
    17161 caaacatcgc ctcgtctgac atagccatca acagtcctta ttaatgagaa atccttctca
    17221 cgcaattttt ctggccatca gatagatggc cggttcctgc acaatttcat ccaacagatg
    17281 aatgaattga atgctacgcg cctgccacac agacgtcgcg tcatctgcca gtagcgcgaa
    17341 tgcccgggtc aacagggcgg tgtggctggc gtcaataaca atctcaccaa aggtcatcac
    17401 ccccagcatc ttacgtcggg cttgctcctc ttcgacctgt gcaagccaat cttcgtattc
    17461 cgtcagtggg cagcggaagg cgacattcag acaatcaatc acgccaatgt gatggccgat
    17521 agccagcgag taatacatta cctgagagct gtgttccggc atctcgtcgc tactggcgac
    17581 aaactttttt cgcagcgtcc agaaaacaat ttccccgcac tcttcagtca tgtaaccccc
    17641 ggagtacgcg aaagagttgc tgaacgatac tattgagacg tggatcgtcg gcatcgcgca
    17701 accaggtgtt aacccgattt cctgtaggat cgtcgcttaa atggcgcagg agccgatcgc
    17761 aaatttctcg cccctgacga tagcccgcca gccgccgcgc ttctcgctca atggcgatac
    17821 gctgtgatgg cgggatctgt ggccacaacg gttgcatagt caccccggtg ggatcgcgat
    17881 aatccacagc gtgaatcttc tgttgcagca aaccgagcgc cacggcgaaa ccgtgaatgg
    17941 tggcggccgg tgttggcggg cagccgggga tccaaacatc aatggggaca atggtgtcgc
    18001 tgccgcccca gacgctgtag agatcgtgga aaataccgcc gccgacaccg cacgcgccgt
    18061 aggaaacaca gattttatga tcgggggcag actcatacgc ccgaagtgca ggcatacgca
    18121 tcgcccgggt gactgcgcca gtaaataaca aaatatcggc gtgacgcggt gatgaaacaa
    18181 ccttaatgcc aaaacgttct gcgtcgaata cgggggtgat ggcagcaaaa atttcgattt
    18241 cacaggcgtt gcagccgccg caatcgacgc gataaacgta ggctgagcga cggatatcct
    18301 gtagcaaatg ccgcttcatc ttttgcgttt gctcatccag cgtgatgggc tggctgacat
    18361 gttgtgtaag cactggactc atagctgctc cttagccacc agcggtacat ctgtatcgtc
    18421 gttgatcagc gtcgcgcgtt gtttgcactc cgggcagacg ctcgcttgcg cccataacat
    18481 ttcgcggttt tgtggcgcat tttgttgctg tgccaacaat tcagcagcca gtgcgacggt
    18541 tttttgctgg gcaaacgggc gttcgcaacg gctgcaacgt tgtagatgga acgtcgcgcg
    18601 ggtatagaga tcggctttat tggtgacggt cagttcaaag ttattggtaa gctggatggc
    18661 tctggtcggg cacacttctt cacaacgtcc gcagtaaata caacgcctca gatagagctg
    18721 ccaggtgcgc gaattttgct ggtcgtcggt ctggatagtc agcgcatttg ccggacaagc
    18781 acaggcgccg caggcaatac attggctggg catcaggtcc ggtttcccgc gaaagcctgg
    18841 gctgacctcc agtggcgcga agggatattt caccgtcgcg gttccggcgc gcataatagt
    18901 tttcagtaac ttcagcatct tctgtcctta cttcagcggc gaacggttac gatcaatgcc
    18961 gtagcgttcg atctctttat acggcacggt ttttgactgg cgcttgcgca catctaccag
    19021 cgtcacacgg tcggtacagg agtagcaggg atcaaggcta ccgataatca gcggtgcgtc
    19081 agaaacggta ttgccgcgca gcatgtaacg caacaccggc cagttggcgt aggtggcggc
    19141 acggcagcgc cagcggaaca atttttggtt atcaccgagc atgctccagt gcacgtcttc
    19201 accgcgtggc gcttcaacaa agcccagcgc gaatgcgtga ggtttatagc taaagccttc
    19261 ggtcagcagt ggggtatccg gcatgttgtc gagggcaaat tccagcattg ccagcgaatc
    19321 aaatgtctct ttgacacgga ccatcacgcg ggagaaaaca tcgccgccgg taaaggtaaa
    19381 cagtgttttt ggaatattgc cgtagtcggc gtaggggtga tcaaagcgca aatcacgggc
    19441 gatttgtcgg tcgagaatgc caatgccctg agtgcgttgt tccatattcg gcgtagcaag
    19501 cagcatctct acccgctccg acacgtcggc gcgcatctcg cgcaccagtt tcagcgtttg
    19561 cagacgttgc tctttgagaa tatcgcggcg aacaccacca atcagattca gaccgtaggt
    19621 tttacgcgac ccgatcagca attccgccat cgtcatcgac ttttcccgca cgcggaaaaa
    19681 ttgcataaag ccggtatcaa aaccaacgaa atggcaggag aggccaaggt taagcaaatg
    19741 actgtgtagc cgttcgactt ccagcagaat cgagcgaata gtatgtgctc gttgcggcac
    19801 ctcaatcccc agtgcatttt caaccgaatt ggtataggcc acactgtggg caaaaccgca
    19861 aatcccacac acgcggtccg ataagaaggt cacttcgttg tagcccatcc gcgtttctgc
    19921 cagtttctcc atgccgcgat ggacataaaa caggcggtaa tcagcatcga caatttgctc
    19981 gccatccaca aacaagcgga agtgacccgg ttcatcggag gtgatatgca gcgggccgac
    20041 agggatcacc cgcgcatcgc tgttgccctc attgataaac gcatacgttt cggaatctgt
    20101 cgtcggttca gggcgcagtc gataatccat cgcatctttg cgcagcggat gcatatcttc
    20161 cggccagtca tcgggcaaca ccaggcgacg ctgatccggc aggccaaccg gaatcagccc
    20221 gtacatatcg cgaatttctc gctcgcccca gaccgcggca gggacgcgcg gtgtgactga
    20281 cggaaactcc cgactgtcgg catcgaccag tgccttcacc acgatccagc atttttcggc
    20341 cccttccatt gaaagggcat aataaaccgc gtaatgaccg ttaagtgtcc gctcgtcgtt
    20401 gccaaacagg actggaagcc agccatcatg ttgataataa agataatgta caacgtcagg
    20461 cagcagattg attttcaccg taatggtgac ctgttcaggc gtttgtcgct cttcatccag
    20521 caccgcgccg gggaactgcg ttttcagggc ggcgagaatc gcttcgccac gatttgacga
    20581 tgaattaacg ttcactgtgt ttctccgaaa cagatgcggt gcctgaaggc aaaaaatcat
    20641 gccaggtgct acgttgtgca ggcaggtcgt gcgtccctga gaggactata gtggaagcgc
    20701 ccgccaggat cctgatgaca ggttgtggaa tatgcgttcc catcgccagc atcatgacca
    20761 gcagaatcac cattggcgag gtggtcaacc agccgagatc accccggtta acggcctgcg
    20821 gcggtttcgc cattaacacc cgcgcagcca tccgtaccag gcccgccagc accagcgtta
    20881 acagcaataa cagcaggacg ataatcagca ggtgattacg tgccagtcct gcggtaacgg
    20941 tcataaattc gctaagaaaa atgttgaagg gcggcatccc tgccagcgcc agcgcaccgc
    21001 cgccaaacag cacggcggta aatggcatga ttttgagcat cccacagacg acgttgagat
    21061 cgcgcgtgcc gtacttgagc agtacattgc cggaaccgca gaacagcagc gtttttgcca
    21121 gactgtggtt taaggtgtgc agcagcgcgg caaaaattcc cagcggcccg ccaatgccta
    21181 gcgccaccgc gaccagcccc atgttctcca cgctggagta cgccagcaga cgcttaatgt
    21241 cccgctgtac cagaatgaaa aatgccgcca cggcaaccga caacatgccg aagatgagca
    21301 acaaccggtt ggggaaatcg ctgccgatgg cttggcaaat aatgatgtaa tagcgaatca
    21361 gcaccaacag cgcgcagttc agcaatacgg cggagagcag ggcgctgacc ggactcggtg
    21421 cttcactgtg agcatccggc agccaggcgt gcatgggaaa tagcccggtt ttggtaccaa
    21481 agccaattag cacaaacaca aaggccaaca gcattaatgt tggttcaagc aaggacgatt
    21541 gctttagaac ctcgctccag aatatcgcca tttctgcctg cggcataacg ctggcggcgt
    21601 tggcgtatac cagcacggta ccgaacagac caaaagcgac accaacagta caaataatga
    21661 tgtacttcca tgctgattcc agcgatgaac gctgaccgta aatgcctacc agaaacgccg
    21721 agcttaaggt ggtggcttcg atcgccgccc acatcacaat caggttattg ctggtaacaa
    21781 ccagcagcat ggtgaacaaa aacagatgga agaaaccgta gtaatcgcac agcgtaacgg
    21841 gtgaaagctc gccgtgtgcc acttcatgac gcatgtagcc aatcgagtaa acaccggtga
    21901 gaaagccaat cacaccaaga atggcgagga acaaaccgcc cagaccatca atatgaagcc
    21961 acagtcccgc agcgaatatt tctcctgcat cagcggcagt ttggaccacc cagagtgcca
    22021 gaatcagcag cagtgtgatc cctaagctat gtaatacagt caccgtacaa gtcgcagaga
    22081 gcccccgttt ccggcaggca aaacagagca gcgaaaaaag cagcggcgtg agcaggagta
    22141 aagcgaacat cacagaataa ctcatctcat tatcccttca gcgcggtcaa gttgttcacg
    22201 tccagcgtgc cgtgggtacg ccatattttt cttgccagta acaccatcac aatgacggcg
    22261 aagatggcgt cggtagcgat acctatttcc accagttccg gtgctcgcca ggcaagaagc
    22321 gccagcacca gatgggagcc gttttccatc aggcagtaac caaaaatttg ccgcaggata
    22381 ttgcgctggc tgacaatgca cagcaggcca agcagaaaat gacctaacgc taccgccagc
    22441 gccggtttca gcccggtagc catcggtagc ttcacgggct gaacgacaaa tgcgcaaagc
    22501 aggacaatta acgccgcgag tagtgccatc attgccggac cgaataacgc tttttccggg
    22561 atgttctggg gaatatttcg tgcagcgtaa gtcatgatta acggtaccag cagcactttg
    22621 gtgataaagg cgctggcgga ccagatcagc agttgctctg cggcgaacag gcacgagaga
    22681 gtggcgaaaa tagacaccag caccagcgac tggcaggcgt aaaatccgca tgacaggcga
    22741 tagcttttga cgctaatcac aaacagcgat gtcagcatca tcagtcccgc cagattattt
    22801 acgatcatag aaccggtcat tcatcatctc cttactgcaa ccaggtcgcg gcgattacgc
    22861 tggaaatcag cgacatcaca atcagtacaa tcaacaccag gcgcattgac cctggcagcg
    22921 gtgcggcatc ggcgacggcc tcgctcggtt tgccaggcac aacgcgacca aaccagcgaa
    22981 taaaccaggc gaaactggcg accgattcaa tcatcagcag aatcatggcg ggcagcagga
    23041 tccagtactc cactgacaac gcaaaaccgg cagcaaacag cgggaattta ctgaagaagc
    23101 cgttgaacgg cggcacgccg gtaatcgcct gcgctgccac gcagaaaccc acgcctggca
    23161 acggcagagt gtgcaatacg ccacgcagac gcggcaacaa gcgcgtgccg cagctgtaac
    23221 tcagcgcacc tgctacaagg aaaaacaggc ttttagcgaa cgcgtggttg acgatgtagg
    23281 cgatgctgcc ctccagcgcc agccgcgagc cgaagatgga gagcgacaaa ccgaagaaca
    23341 tccagccaag ttgagtgatg gtcgaccagg ccagcaaccg cttcatatcc tgctgtggca
    23401 aatacatcag aaatccataa agaatggtga ccagcgccat gaccatgcca acgccgccaa
    23461 tcacatgcgg gatattgccg ccgtcgataa tggcgcgggc aaaaatgtaa acgcccactt
    23521 tcaccttcga tgcggcgtgg agataggcac tgatcggtgt tggcgcttcc attgcgtccg
    23581 gtagccacgc ttgcatcggt agctgggccg atttccccca cgcggcaaac aggatgccgc
    23641 cataaaccag ataacgtgcg tcgccgtgta actcgctcat cgcgctaagc gcaaacgttc
    23701 cggtctgcaa aaacagcgtg gcggcggcaa gatacaaccc caacgagccg atatgagtga
    23761 taagtaacgc ttttagtgct gaaagctgtg ctttatcgct ctggtaataa ctgatcaacg
    23821 cccaggagca gccgcccgta atttcaaaaa acaacaactg accgagcagc gtcgaggaga
    23881 gtaccagtcc cgccatcgcg ccgataaaca ccagcaaaaa tgcgtaataa cgattcgtgc
    23941 cgttatgcgg gtgttcgcga tttttatccg tcagataacc cgtggagtag atcgtgacca
    24001 gcaaaccgag aaacaccacc acaaacagaa tcagcgtact cacgcgatca atgaccaggc
    24061 caaacaatgc cacatccccc acgttgacca acgtcaacgt gacggcaact ttatcggcct
    24121 gataaaacgc ggagatcagc gacaacatgc acagcgtggt cagtgcggcg aacaaaaccc
    24181 ccattcggcg gcccgacgtt gtggcgaaaa cgaaacgacc agtgcgccga taaaaggcag
    24241 cattaacgtc gtcagagcaa gattttccat attccgtcag tgctccttac agaccggtta
    24301 accagaagac ccatgcaagc gcagcaaggc tgaagccaag ccaggtcaca tggtgaatga
    24361 gtaaaaaacg cccgcgtgcc agcgtgtttt ctgcgattga ggccagtaca aaaatcagca
    24421 aaaccttgag cagcgtaacg acaagtgaag tcagcaggca ggcgagagaa agttcttgcg
    24481 cgcgcccaaa gggcagaaac agggccacaa acagtgctgc catcacgacc tgtttcagcc
    24541 ccagccccca tttcgctagc gccagcccgg caccggaata ttcggtcagc gggccttcct
    24601 gtaattcctg ttctgcttca gcaacatcaa agggaatttt tcccatctca atgaagcagg
    24661 caaaaccaca ggccagtaac gccagtacgg tggttagcgg cgagttccag cccgttgcca
    24721 gcgtattgct gatcatctcg atatgcgtgg aacctgctat cagcgccaat accagcattg
    24781 agagaatgag catgggttcg accagaatgc cgagcgtcaa ctcgcgactg gcaccgactc
    24841 ccgcaaacgg gcttccggta tccagcccgg aaagagcaaa gaaaaaacga aacagggcaa
    24901 gaagatagat gagggtgatc agatcgccgc cgcccgcaaa aggggaaacg gtaataaaaa
    24961 gtggtagggc catcgccagc accagcatgc tgctgattaa tacccacggc atcaggcgga
    25021 acatcagacc tgaagatata ggcgcaactt cctggcgttt aaacagtttg tggatatcgc
    25081 gataatcctg ccagatcccc ggcccgcggc gggagtgcat acgcgcgcgt atctgccggg
    25141 aaatacccgt aaaaagcggg gttagcagca gcagaatcac ggcctgtgct aacgcaaaaa
    25201 tgaccagata tccgtcgcaa agagtttgtc tcatggtgat ttccttagac ggcaatagcg
    25261 attagcagca caaccagtgc ggcgaccaca tacaggcaat agagacgaaa gtcgccgctt
    25321 tgcagatgct gtatttcttt ggccagccgt tgggtggcgc tcacgatggg gcggatcacc
    25381 cgctcatccc agaagggttc tgtgctctga gccctggcgg tgacgtgcgc aagaccttta
    25441 tttagcctca gcgtagggtc gagctgttgt cgtagacgaa atagcgcaga aaagaccaca
    25501 cgcagcggct gcatcacgcc attgcctgac ggggccatcg cattttccca gccgtagccg
    25561 catgcccagg catctcctgt gcgacgaaac gctgcgcggc gcgaacgaca ccacagccag
    25621 taaaggccag gcagcaaagg catcgccagt agtaacagca acagtaatga gggtgtgacc
    25681 cgtgtatgaa acgtgccggg tacaagtgct attccgctgg cgacagtgac gggaggggta
    25741 ttggtaaacg catgggcaat gtgcattatc ttcggtgcca gccaactggc actaagcgca
    25801 attaatacgc agagtgccgc gagcagtagc atggcgacga tcatcgtatt tggcacttcc
    25861 tgtgcctctt cagcgtgtgt gctgcgcggc gcaccacaga aagtaatacc gtacattttg
    25921 acgaagcaca ttactgccag cccaccagtg actgccagca ttaccatagc aataggaccc
    25981 gcaagttgta gcgctacggc ttccacacga cttagtgaga acagcgactg ccaggtgtac
    26041 cattcgctga taaaaccatt cagcggagga atggctgata tcgccaggca accaatcagg
    26101 catgctgcgg ctgtccacgg catccgtttc gccagtgccc ccattttttc catgtcgtgg
    26161 gtatgcaaac gcgaaataat cgcacccgcg ccgagaaata gcagcccttt gaacagcgca
    26221 tggttgagca gatgaaacag tgcgccgagc agtccaacca cggtgagcag cgggtcgtgc
    26281 agtgacagac cgtccatcgc cacaccgacc gccagcaaaa taatgccgac gttttcgacg
    26341 gtactccagg ccagcagccg tttgatatcc tgttccgcca gtgcatacag cacgcccagg
    26401 agcgcggaga ttgcgccgat cgccatcacc agagtgcccc accacagagg caaacccgtt
    26461 tgcgccagca gatccatcgc tactttcagg atgccgaaaa taccgatttt gaccattacg
    26521 ccagacatca acgccgaagc gtgcgatggt gcggcagggt gagcgcgcgg caaccagctg
    26581 tgcaacggca tcatcccggc tttcgcgcca aaaccgaaaa aggccagcag aaacaccgcc
    26641 gacgccagcc ccggagaaag tgaaagcgtg cgaaactggc aaaatcgagg ctgccgcttt
    26701 cgcgccccat cagcaagaag gcgatcataa tcagcaccga tccggcgtgg gcgataaaaa
    26761 agtagagcat gccagcgttg atcgacgttt tatcctgcct ggcaatgacc agaaaccagg
    26821 aagacagcga catcatttca aacagcacga tgaaccaaaa agcgttgtcc atcaccagca
    26881 gggcaaccat cgatgcgatg aaaagattca taaagaagcc catcgccgcc gcgcctttgc
    26941 cctcgtattc gcgcatataa gtcaatgaat agagcgaaca aaccaccacc agcaaggaga
    27001 taaccagcac cataaatgcc gagaggctat ccattcgcag ggttaaatcg gcaaagtcaa
    27061 aagggggggc aaaatgggcg actaaagtta caccagcatg taattgcgta attccgctga
    27121 tgatgccaat cacgccgccc accagcgatg tgatgccgga aagcttaata gccagtctgt
    27181 ccagaccgag taaaaacagc gaagccagat tggcaaacag atagaggatc agcgaccagg
    27241 ttaataattg cagggcatcc attagtgttg ctcctgagtg agggaaaggg gatcctcccc
    27301 gccattggca aaccagcttg cggcaaggcg ctgtttttct ttcatctgac gttgcaggct
    27361 atcgtcggtg atcagccgta acgcctgatt cgggcaagcg cgaacgcagg ccggaccttc
    27421 tggcaagaaa tcacacaggt cgcatttcac cgcgacggtc tggacacccg gttcccagcg
    27481 cagcaacgca tgttggggca gcacgttttc ttcgccgttt ttgagtgagc cttcagcctg
    27541 aaaaacatat tgcgcatggg cattcaccgg acggcttcct gaagcgctga ttgcgccaaa
    27601 tgggcagacc acggcgcaaa gcttgcagcc aatacagagg ctttcgttga gttggatcgc
    27661 atcatccctc tgagagatgg cattgaccgg gcagacctgc aggcaagggg cttcctcaca
    27721 gtgatgacac acgacagggg cagtgattgt tgacgtcttc gccagggcca ggcgcgggtg
    27781 ttgctgtaaa ccttgcgttt tatgcacgtc cgaacaggca gcgagacagg tattacatcc
    27841 tgtacaccac agtggttcgg ccaccacaaa gcggttcatg tcggctcctt gaaaatgata
    27901 tgaaacattg catttcatat tgcataaagg atgccattct gattaactca atatattcaa
    27961 taagttatgt attgactgga ttctgaacgg tacaaaaatg atggtcatta acgttcagca
    28021 aaaaatggat tcgacacccg tgtcgcaggg gaaagcgaga atgtgatctt gatcacgaga
    28081 agtgaggtat gctggccgca agcgcagcca gcacggaatg gagcgaagta atcaggcgtg
    28141 ttctttcagc cagttcagca caacgtcgtg gtgattgctg gttttgaaat cgtcaaagac
    28201 atgttcgatt ttgccatcag cgtcaatcag gaagctgatg cgatgaatgc catcgtaggt
    28261 tttgcccatg aaggactttt caccccagac gccgaattgt tcgcacacct ggtggtcctc
    28321 atcagacagg agcgtaaagt taagcagctc tttttccgca aaacgggaga gtttttcggg
    28381 tttatcggtg ctgataccca gcacatcaac gcccgctttt ttcaactcat ccatgttatc
    28441 gcgtaagccg caggcctgta cggtacagcc gggggtcatg gctttcgggt agaaataaac
    28501 cagaacacgc tgtccctgga agtcggtcaa attaacttgt tctccgtctt gatccggcaa
    28561 gctaaatttc ggtgcgatat caccggcttt cagtggattc attacttaac tccatcctgt
    28621 tcatcatgtt gggaataatt gacgacgtta atactgcctt gtgcattgag ttctgtacat
    28681 agggctttga acgcttgctc aatatttgct gcgtccgcag atgcggggct gtgggcggtt
    28741 atctgaatat gcaactgcgc agcccgttca ttttcagcag gttgcgtgcg cgacaccagc
    28801 tccgcaatgt tcatatgatg cgcgtcgaaa agtgctgtga agcgttcaat taaatgcggg
    28861 gagtctgcca catcgacctg aacccagaca gatgctggca ttggcggacg cggacgcgcc
    28921 gtcgtgcgct tcatcacgat taaaagatcc agttcggcac ctttcaacgg taacgttgat
    28981 tcaatcagag tgatggcatt ccatgaaccg gaaagcagca tgataaacgt gaactcttct
    29041 cccagcatcg ccaggcgact gtcttcaata ttacagccgc aactactgac atgacgggtg
    29101 atggtgttca caattccagg gcgatcggca cccaacgcag tgatcaccag ataatgttgc
    29161 gatgacagtg tcaaactggt tattccttta aggggtgagt tgttcttaag gaaagcataa
    29221 aaaaaacatg catacaacaa tcagaacggt tctgtctgct tgcttttaat gccataccaa
    29281 acgtaccatt gagacacttg tttgcacaga ggatggccca tgttcacggg aagtattgtc
    29341 gcgattgtta ctccgatgga tgaaaaaggt aatgtctgtc gggctagctt gaaaaaactg
    29401 attgattatc atgtcgccag cggtacttcg gcgatcgttt ctgttggcac cactggcgag
    29461 tccgctacct taaatcatga cgaacatgct gatgtggtga tgatgacgct ggagctggct
    29521 gatgggcgca ttccggtaat tgccgggacc ggcgctaacg ctactgcgga agccattagc
    29581 ctgacgcagc gcttcaatga cagtggtatc gtcggctgcc tgacggtaac cccttactac
    29641 aatcgtccgt cgcaagaagg tttgtatcag catttcaaag ccatcgctga gcatactgac
    29701 ctgccgcaaa ttctgtataa tgtgccgtcc cgtactggct gcgatctgct cccggaaacg
    29761 gtgggccgtc tggcgaaagt aaaaaatatt atcggaatca aagaggcaac agggaactta
    29821 acgcgtgtaa accagatcaa agagctggtt tcagatgatt ttgttctgct gagcggcgat
    29881 gatgcgagcg cgctggactt catgcaattg ggcggtcatg gggttatttc cgttacggct
    29941 aacgtcgcag cgcgtgatat ggcccagatg tgcaaactgg cagcagaagg gcattttgcc
    30001 gaggcacgcg ttattaatca gcgtctgatg ccattacaca acaaactatt tgtcgaaccc
    30061 aatccaatcc cggtgaaatg ggcatgtaag gaactgggtc ttgtggcgac cgatacgctg
    30121 cgcctgccaa tgacaccaat caccgacagt ggtcgtgaga cggtcagagc ggcgcttaag
    30181 catgccggtt tgctgtaaag tttagggaga tttgatggct tactctgttc aaaaggcgct
    30241 cctggcaaag gttgcgggtg tttcgcttgt tttattactc gctgcctgta gttctgactc
    30301 acgctataag cgtcaggtca gtggtgatga agcctacctg gaagcggcac cgcttgcgga
    30361 gcttcatgcc ccggctggaa tgattttgcc ggtgacctcc ggtgattatg caatcccggt
    30421 gaccaacggt agtggtgctg tcggtaaggc gctggacatt cgtccaccag cccagccgtt
    30481 ggcactggtt tctggcgcgc gtacccagtt cacgggcgat accgcttcat tgctggtgga
    30541 aaatggtcgt ggcaatactc tgtggccgca ggtggttagc gtgctgcagg cgaaaaacta
    30601 caccatcacc caacgtgatg atgctggtca gacactgacc accgattggg tacaatggaa
    30661 ccgtctggac gaagacgagc agtatcgtgg tcgttatcaa atctctgtta agccgcaggg
    30721 ttatcagcag gcagttacgg ttaaactgct gaacctggaa caggcgggca aaccggttgc
    30781 agacgcggct tccatgcagc gttacagcac ggagatgatg aacgttattt ccgccggtct
    30841 ggataaatct gccactgacg ccgcgaacgc tgcgcaaaat cgtgcctcca ccactatgga
    30901 cgtacaaagt gcagctgatg acaccggttt accaatgctg gtcgtacgcg ggccgttcaa
    30961 tgtggtttgg caacgtctgc cagcggcgct ggaaaaagtg ggcatgaaag tgaccgacag
    31021 cacccgttcg cagggcaaca tggccgtaac ttataagccg ctgtctgaca gcgactggca
    31081 ggaactgggc gcgagcgatc caggcctggc atccggtgac tataaactgc aggttggcga
    31141 tttagataac cgcagcagcc tacagttcat cgatccgaaa ggtcatactc tgactcagag
    31201 tcagaacgac gcgctggtat ctgtcttcca ggctgcgttt agcaagtaaa aatacagggc
    31261 tggaatcatc cggccctttt ttctgatatg acacgcaaac gtgtgcgtag gcaggaaaac
    31321 gcgattttag cggtaattcg cacgaaattt gtttgtcgga cgtagttcga ataaggcgtt
    31381 cacgccgcat ccgacaaaac atccggcaca ccagacagca aaagatttta aaacgttaat
    31441 tcacacccag gagtgataaa gatgcaaaag caagctgagt tgtatcgtgg taaagcgaaa
    31501 accgtataca gcacggaaaa cccggacctg ttggtgctcg aattccgcaa tgatacgtca
    31561 gcaggggatg gcgcgcgcat tgagcagttt gatcgcaaag gtatggtgaa caacaagttc
    31621 aactacttca tcatgagcaa actggctgaa gcgggtatcc cgactcaaat ggagcgtctg
    31681 ctctccgata ccgaatgtct ggtgaaaaag ctggatatgg tgccggttga gtgtgtcgtg
    31741 cgtaaccgtg ctgctggctc tctggtgaaa cgtcttggaa tcgaagaagg tattgagctg
    31801 aacccgccgc tgttcgatct gttcctgaaa aacgacgcca tgcacgatcc gatggtcaac
    31861 gaatcttact gcgaaacctt tggctgggtg agcaaagaga acctggcgcg tatgaaagag
    31921 ctgacctaca aagcgaacga cgtgctgaaa aaactgttcg atgatgctgg tctgattctg
    31981 gtcgacttca agctggagtt tggcctgtac aaaggtgaag tggtactggg cgatgagttc
    32041 tccccggacg gcagccgcct gtgggacaaa gaaacactgg agaaaatgga caaagaccgt
    32101 ttccgccaga gcctcggtgg cctgatcgaa gcctatgaag ccgtcgcccg ccgcctgggt
    32161 gtacagctgg actgattttt ctggttgctt gtttctttag ccggagggta ctccggctaa
    32221 atgtctttga tacccccgct acaatacttc tcattatctc cctttgacag gaaagtcata
    32281 ccaggccaat atgtatatac ataacgtata tcccaaaaag ggggggctat ggaaactact
    32341 gtatttttaa gtaatcgcag tcaggccgtc agattaccga aagcggtcgc tctgccagaa
    32401 gacgttaaga aagtagaaat tattgcgatt ggtcgtacaa gaatcatcac tcccgccggt
    32461 gagtcctggg acagttggtt tgatggcgaa aatgtgagtg cggattttat ggatatccgt
    32521 gatcaacccg caatgcagga aagggaatca ttttgatgct gaaattcatg ctggatacca
    32581 atatctgtat tttcactatt aagaataaac cagtacatgt cagagagcgg tttaacctga
    32641 attctggccg gatgtgtata agttctgtaa cattgatgga gcttatctat ggggcagaaa
    32701 aaagtcagat gccagaacgc aatttggcgg ttattgaagg tttcgtatct cgtttggtag
    32761 tgcttgatta tgatagtgct gctgcgacgc ataccgggca aatcagggcc gagcttgccc
    32821 ggcagggaag gcctgttgga ccttttgacc agatgatagc gggacatgct cgcagtcgcg
    32881 gtttgattgt ggttaccaac aatacccgag agtttgagcg agtggcggga atcagactag
    32941 aagactggag ctaacattct cttttacttc taacattcgt aaattatata acgtggcatg
    33001 aagccttaat tcctccgcca tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac
    33061 ctacgatcat ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg
    33121 tgaaagtgac aatgttgaag acaggcgcaa cagctccggt ggtccgtcta tgggcggtcc
    33181 cggttttcgc ctgccaagcg gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc
    33241 aggctactat ggtgttgatt taaccgggtt aatgactgga cagccggttt cccaacaaca
    33301 atcaacgcgg tccattagcc ctaatgaaga cgaagctgca aaattcacct cggtgattct
    33361 ggcaaccacg gaagacacct ggggacaaca gttcgagaag atgggtaaga cctatcagca
    33421 accgaagcag gtcatgtacc gtggaatgac gcgcaccggc tgcggggctg gtcagtccat
    33481 tatggggccg ttctattgcc cggcggatgg cacagtttat atcgatctct ccttctatga
    33541 tgacatgaaa gacaaacttg gcgcggatgg cgattttgcc caggggtacg ttatcgccca
    33601 tgaagtcggt catcatgtgc agaaactgtt aggcatcgag ccgaaagttc gtcaactgca
    33661 acaaaacgcg acgcaggcgg aagtgaaccg cttatctgtg cgtatggaac tccaggccga
    33721 ctgttttgcc ggtgtctggg ggcatagtat gcagcagcaa ggcgttctgg aaaccggcga
    33781 tctggaagag gcgctgaacg cggcgcaggc catcggcgat gaccgtttac aacagcaaag
    33841 tcaggggcga gtagtaccag atagtttcac acatggcact tctcagcaac gctacagctg
    33901 gtttaaacgt ggtttcgaca gcggcgatcc ggcacaatgc aatacttttg gtaaaagcat
    33961 ttaacttccg gggcagggat ggctgaactg actgcgcttc acacattaac agcgcaaatg
    34021 aaacgtgaag ggatccgccg cttgctggtg ttgagcgggg aagagggttg gtgttttgat
    34081 catgcgctta agttgcgtga tgccttacct ggcgactggc tgtggatttc gccgcagcca
    34141 gatgctgaaa accactgttc accctcggcg ctacaaactt tacttgggcg cgagttccgg
    34201 catgcggtat tcgacgcccg ccacggcttt gatgccgctg cctttgcagc acttagcgga
    34261 acgttgaaag cgggaagctg gctggttttg ttactccctg tatgggaaga gtgggaaaac
    34321 caacctgatg ccgactcgct gcgctggagt gattgccctg accctattgc gacgccgcat
    34381 tttgtccagc atctcaaacg cgtacttacg gcggataacg acgctatcct ctggcggcaa
    34441 aaccagccgt tctcgttggc gcattttact ccccgtactg actggcaccc cgcgaccggc
    34501 gcaccacaac cagaacaaca gcaactctta cagcagctac tgaccatgcc tccgggcgtg
    34561 gcagcggtaa cggctgcgcg tgggcgcggt aagtcggcgt tggcagggca actcatttct
    34621 cgtattgcgg gcagtgcgat tgtcaccgcg cccgcaaaag cggcaacgga tgtactggca
    34681 caatttgcgg gcgagaagtt tcgctttatt gcaccggatg ccttgttagc cagcgatgag
    34741 caagccgact ggctggtggt cgacgaagcc gcagccatac ctgcgccgtt gttgcatcaa
    34801 ctggtatcgc gttttcctcg aacgttgtta accactacgg tgcagggcta cgaaggcacc
    34861 ggacgtggtt ttttgctgaa attttgcgct cgctttccgc atttacaccg ttttgaactg
    34921 caacagccga tccgctgggc acagggatgc ccgctggaaa aaatggttag tgaggcactg
    34981 gtttttgacg atgaaaactt cacccataca ccacaaggca atatcgtcat ttccgcattt
    35041 gaacaaacgt tatggcgaag cgagccagaa acgccgttaa aggtttatca gttattgtct
    35101 ggtgcgcact accggacttc gccgctggat ttacgccgga tgatggatgc accagggcaa
    35161 tattttttac aggcggctgg cgaaaacgag attgccggag cgctgtggct ggtggatgag
    35221 gggggattat ctcaacaact cagtcaggcg gtatgggcag gttttcgtcg cccgcggggt
    35281 aatctggtgg cccagtcgct ggcggcgcac ggtagcaatc cactggcagc gacattgcgt
    35341 ggacggcggg tcagccggat agcagttcat ccggcgcgtc agcgggaagg cacagggcag
    35401 caacttattg ctggtgcttt gcaatatacg cgtgacctcg actatctttc ggtgagtttt
    35461 ggttacaccg gggagttatg gcgtttctgg caccgctgcg gttttgtgct ggtgcggatg
    35521 ggtaatcatc gtgaagccag cagcggttgc tatacggcga tggcgctgtt accgatgagt
    35581 aatgcgggta aacaactggc tgaacgtgag cattaccgtt tacgtcgcga tgcgcaagct
    35641 ctcgcaaagt ggaatggcga aacgcttcct gttgatccac taaacgatgc cgtcctttct
    35701 gacgacgact ggcttgaact ggccggtttt gctttcgctc atcgtccgct attaacatcg
    35761 ttaggttgct tattgcgtct gctacaaacc agtgaactgg cattaccggc gctgcgtggg
    35821 cgtttacaga aaaacgtcag cgacgcgcag ttatgtacca cacttaaact ttcaggccgc
    35881 aagatgttac tggtccgtca gcgggaagag gccgcgcagg cgctgttcgc acttaatgat
    35941 gttcgcactg agcgtctgcg cgatcgcata acgcaatggc aattttttca ctgactcctt
    36001 caattttctc ccatggtcat tgtgagtcag cggcgtaaaa ttaatgacat cagttaagga
    36061 gttcgccatg aaacatgacc attttgttgt tcaaagcccg gataaacctg cgcaatagtt
    36121 gttactgctt ttccacggtg tcggggataa cccggtggca atgggggaaa taggtagctg
    36181 gtttgcaccg ctgtttcctg atgcgctggt ggtgagtgtc ggtggcgcgg agccgagcgg
    36241 taatccggcg gggcgtcagt ggttttcggt gcagggtatt acggaagata atcgccaggc
    36301 gcgtgtggat gcgatcatgc cgacgtttat tgaaacggtg cgctactggc agaaacagag
    36361 cggagtaggg gctaacgcca cggcgctcat cggtttctct caaggcgcga ttatggcgct
    36421 ggagagcatt aaagccgaac cgggccttgc ttcacgcgtc attgctttta acggacggta
    36481 tgcgagcctg ccggagactg cgtctacagc cacgacaatt cacctgattc atggcggtga
    36541 agatccggtt atcgatctgg cgcatgcggt tgcggcgcag gaagcgttaa tcagtgccgg
    36601 tggtgacgtg acgctggata tcgtggagga tttaggccat gcgatcgaca atcgcagtat
    36661 gcaatttgcc ctggatcact tgcgttatac cattccgaaa cattatttcg acgaagcgct
    36721 aagtggcggt aagcctggcg atgatgatgt gattgaaatg atgtaaaaga cagattacct
    36781 ggcctgataa gacgcgtgag cgtcgcatca ggcatcgttc accattgccg gatgcggcgt
    36841 taacgcctta tccggcctac ggttgagcac tcagcttact tcttcggttg atcctttttc
    36901 ggccagtcgt cgtcatcgtc ccacttatcg ttgaaatcac gatgcggggg aagctccggc
    36961 ttgttggcga gaaatttctt atggtcgacg cgcttgagat ctttaatcac gttcagcagg
    37021 acgcctacca aaaagacaat caccagaatc caccaatatt tagccagcca gtccatgctt
    37081 atttcctctt gcagaaccac tcatcaggcg acgagctgtt ccatgatacg ttgatacata
    37141 cgggcaagta gctgcaggtc ggcagcgttc acacattcat taattttatg aatagtggca
    37201 ttgaccggcc cgagttccac cacctgcgcc cccatgcgag caataaagcg cccgtcggac
    37261 gttccgcctg tggtcagtag ctgcggttta atttcattat agtgctcaac cgcgttaacg
    37321 gccgcatcca ccagtttacc gcgcgcggtc aaaaatggct gcccggaaag ccaccagtcc
    37381 accgtataac gcagttgatg tttttcaagc agggcaagca cctgtgcttt gatcatctca
    37441 tcagtcagtt cggtgctgaa gcggaagtta aactgcacaa acagttcacc cggaataacg
    37501 ttgttactgc ccgttcccgc ctgaatattg gcaatctgca tactggtcgc cgggaagaat
    37561 tcattgccct gatcccactc aatagccact aattcattaa ggaaaggtgc tgcgcgatgt
    37621 accggattgt cagccagatg cgggtaggca acatgcccct gaacgccatg aatggtgagg
    37681 ttgcaggtta acgatccgcg acgaccattt ttcaccacat cacctaccac ttcgatactc
    37741 gacggttcgc cgaccaggca gtaatcgaga cgctcattac gtgccattaa cgcttcgacg
    37801 acttttaccg taccgttgtg tgcactggct tcttcatcag aggtgatcag aaatgccagt
    37861 cgccccgtat ggttgggatg ttgtgcgaca aagcgttctg ccgctaccac catcgccgcc
    37921 agcgagcctt tcatatctgc cgcaccgcgc ccgaataaca tgccatcacg aatggtgggt
    37981 tcaaacggcg gattgatcca acgatcggcg tcgccaggcg gcaccacgtc ggtatgcccg
    38041 gcaaaggcca acgtttcacc ttgcccacgc catgcccaaa aattctgcgt atcggcaaaa
    38101 tccatgcgtt caacggtaaa accgatcgcc tgcaaacgtt caatcaacaa agcctgacat
    38161 cctgcgtcat caggactcag ggaagggcgg cgaataagct gttgtgtcag ctcaataacc
    38221 gggcacgaca tagactacac ctcatggaaa aattgctgat aactggaatc actgaaaccc
    38281 agcagcatag gcttaccggg cgcgcagagc aatggacgtt tgataattgc aggcatttca
    38341 gtcattaatg ccgccgcaga ggccgcatcg gtgattttat tgcgggtggt ttcgtccagt
    38401 ttacgccagg ttgtaccacg ggtgttgagt aacgcttccc agcctaattc gttgataaaa
    38461 ccgttcaata attcgctgtc cagcccatcg acgcggtaat catgaaaacg atagtcgatg
    38521 ttattggctt ccagccaacg gcgagccttt ttaatggtgt cacaattttt gatgccgtaa
    38581 agtgtaacca tgatgaatcc tttttcgatt tatgagaata aaaactcatt taagggtgaa
    38641 aacattttgc ctgcaaatga ttatcaggaa atataacatt atcgaagtat tcaaattaac
    38701 aactattaaa taatattaaa catttttaac gtaaacatta agaatattca tcttgttatc
    38761 tgtctgtatt ttaaccagaa ttaaatcagt attagatgcc gtatgaatat ctcgaaatgt
    38821 tgctgtgtat cacatacaaa ttatcaccgc aggaccatag tatttacata aggtttgtcc
    38881 cccggaggta gtcatcgcag caatcgagta aattagcttc accacggtat attgtttccg
    38941 tagaataccc ataattataa gagaggttgt tatgattgaa cgtgaactgg ggaactggaa
    39001 agactttatc gaagttatgc ttcgtaagta attcctgaga caaagtataa acagcaagaa
    39061 cccgctgagc aggttcttaa tcggaaagtt gaaaaaggcg acacattggc atgtcgcctt
    39121 ttttattgct tattccgggc gcggctttag cgggaagcgg cggcgcacca gcacaaagaa
    39181 cagcggcacg aagtaaatag ccagaatagt ggctgaaatc atcccgccca ttacgccagt
    39241 acccaccgca tgctgaccac cggaaccggc acccgtgctg gttgccattg gcaatacgcc
    39301 gaagataaat gccagcgagg tcatcagaat cgggcgtaaa cgctgacggc aggcgtggag
    39361 cgtcgcttca aacaggtcgt ggcctttttg gttcatctcg ttagcaaact cgacgatcag
    39421 gatggcgttt ttcgccgata aaccaatgac cgttaacagg cccacctgga agtaaacgtc
    39481 gttttccagc ccgcgcatcc aggttgccag cagcgcgccg attaccccca gcggcacgac
    39541 cagcattacc gagaacggca ccgaccagct ttcatacaac gcagccagac acaggaatac
    39601 caccagcaag gaaatggcgt acagcgccgg agcctgcgcg ccggaaagtc gctcctgata
    39661 cgacatcgcc gtccactcca gaccaaagcc gtttggcagc tgcttcacta acgattccat
    39721 aatatccatc gccgtaccgg tactgacccc cggtgcggct tccccaacaa tctcaaccgc
    39781 agaataaccg ttatagcgtt ccagacgcgg cgagcctgtt tcccagcgtg aggtcgcgaa
    39841 agcagagaag ggcaccatgc cgccatcttt atttcggaca taccagagat tgatgtcatc
    39901 tggcagcatg cgatacggcg cagctgcctg cacatagact ttcttcacgc gaccgcgatc
    39961 cataaagtca ttcacatagc tcgaacccca ggcggtttgc agtgtgtcgt taatatcgtc
    40021 gatagcaacg cccagcgcct gagctttacg ctggtcgata tcaatctgca actgcggact
    40081 gtcgtcgagg ccgttatggc gcacacgggt tagctccggg ttttccgccg ccagcgccag
    40141 caactgatta cgtgctgcca tcagcgcatc gtgacccgct ccagcgtggt cctgcaactc
    40201 catatcaaaa cctgcagaac taccaagacc gctaattgct ggcgggctgc tggcgataac
    40261 gcgagcttct ttaatttggt taaacgcctt cgttgcacgc tcgataatgg caaacgaggt
    40321 gccggtctta ctgtcgcgtt cgctccagtc tttcaggcgg ataaacattc gcgccacgtt
    40381 ttgcccgtta cccccagggc cagaaccaac ggtggcaaac accgacatga tgttgtcttt
    40441 ttcatgggtg aagtaatatt tctcgatttg ctcaacgact ttcagggtct gttgttgggt
    40501 tgaaccgctg ggcaattgta ccgaggtagt aaacatgcca cagtcttcca gcggtaagaa
    40561 cgacgtcggc aaacgcagga acaggaacac catgccgcca agcagcagaa cataaatcac
    40621 aatccagcgc aggctacggt ggagaatttt cgccacccct ttttcgtagc gttcggcgtt
    40681 gcggttaaac atctggttaa accaggcaaa aaagcctttt tgcccatgat gctcaccttt
    40741 tttcagtggc ttaagtagcg tggcacacag agccggagtg aggatcatcg ctaccagtac
    40801 tgacagcacc atcgccgcaa caatggtaat agagaactgg cgatagatgg caccggtggt
    40861 gccgccgaag aaggccattg gtacaaatac cgccgaaagc accatcgcaa tcccgaccag
    40921 tgccccctgg atctgcccca tcgatttacg tgtggcttcg cgaggagtga gtccttcctc
    40981 actcataata cgttcgacgt tttccaccac cacgattgcg tcatccacca gcagaccgat
    41041 cgccagtacc atcgcgaaca tggttaaggt gttgacgctg taaccgaagg cgtaaagtac
    41101 ggagaaggtt cccatcaaca ccaccggcac ggcgatagtg ggtatcagcg tggcgcggaa
    41161 gttttgcagg aacagataca taacgaggaa aaccagggcg atagcttcca gcagcgtttt
    41221 caccacgtct tcaatggagg ctttaacaaa cgaggtggtt tcatacgcca ccttgtattc
    41281 cagtccgtgc gggaagtact gcgccagctc gtcgagacga ttgagcacca gctccgctgt
    41341 cgccatttcg tttgcgccgg aggccagttt tacccccagc ccggaggctg gcttaccatt
    41401 gaagcggcta agataatcgt atttctccgc ccccatttcg acggtggcga catcgcccag
    41461 ccttacctct gagccgtctt gattcacccg caaggtgata tcgcggaact gttctggtgt
    41521 ttgcagcagt gactgggcgt taatggtggc gttgagcgcc tgcttatcga cggaaggtgt
    41581 accaccaagt tgcccaaccg caatctgcgc gttctgtgac tcaatggcat cagtgacatc
    41641 tttagccgtc atctggaaac tgttgagttt cgccgggtcc agccagatac gcatggaata
    41701 ttgcgaacca taggcatcga tatccccgac gccattcacg cggcttaacg ggtcctgaat
    41761 attactggca acataatcgg caatatcctg tttatccatc gaaccatcgg tagagacgaa
    41821 agcaatggtc agaatattgg tatcgccggt tttacgcacc gtcacgccct gattttgcac
    41881 cgcctgcggt aactttcgca tggctgattg cagctggttt tgtacttgct gcacggcttc
    41941 gtccggatcg gtgcctgctt taaaacttaa agtgacagat gcctgaccgg tgccactgct
    42001 ctgagatgac atatacatga gattatcgag gccggtcata ttttgctcga taacctgggt
    42061 cacggtgttt tccagcgtct gggccgatgc gccgggatag ttagcggtca ctcgcacatt
    42121 cggtggcgcg agatcggggt attgttcaac gggcaatgag aaaatcgcca gggtacctgt
    42181 cagacacaac aggattgcca gcacccaggc aaaaatgggg cgatcaataa agaaattcgc
    42241 cattaaaaga ggacctcgtg tttctgcgta tcgttgtagg cttgacttgc ttcactgtag
    42301 cggcaaggta cgagccaatc gtggagaaaa aaaggagtta atgtaaatta tcatgccctt
    42361 ttatactgaa ccttaagtgc aagtattctt tggtcagtag gaggcattac atcagctgac
    42421 tttcctcacc ctccgcggag cgaaagctaa tactcactaa cgtgccgccg ccggaaggtt
    42481 gtgaaaaagt cagcgtcccg cccagccgtt ccgcgcgttc gcgcatgata ttcagtccat
    42541 aatgaccttc gggttctttc ggttcaccga taccaatccc gttatcacgg atataaaccg
    42601 tgtgattgcc gtccggcgcg gtgacgcaac tgacggcgat ttcgctggcg ttggcgtgct
    42661 tcatcgcatt cagcaccgct tcgcgaataa tttgcaacaa atgcacctgc atttgcgcat
    42721 ccagcgccag ggttggcaga cggcagtcga gggtcagttt ggcgctggtt tgattttgta
    42781 acgtatccag catttccctc aacgcggagg ggagatccgc ctgctgcagc gtcaggcgga
    42841 aagtagtcaa cagctcgcgt aactgccgat aagcatcatt caacgcctgg gaaaaatcgg
    42901 ccatgatact ttgtgcggtg gcgttatctt ccggtatcga acgcttcagt aacgtcaact
    42961 ggatacgtaa gtaagaaagt acctgagcca gcgagtcgtg caattcgcgg gcgatggttg
    43021 cacgttcttc catcaacaat aactgctgaa aatgcttctg cgcctgatta aagtacaaac
    43081 cgcgtcccag catcgatgaa acactgttaa gcagcggttc tgatgatgaa acgtgactat
    43141 tttgccagtg cagttcgccg taaaccgtct cttgcattgt caccggtaaa atctgcatcg
    43201 gcaattccgg gtttggttgc ccttcgctaa tccgccagtt ttcaccgaca tttaactcca
    43261 gatattcagc cgcttcattg tcgcgaacaa tctgcaaaat atggcggaaa caatgcacat
    43321 caatctggct ggtgttcagc gcttgcgaac attgatacaa cacctccaga cgacgcttgg
    43381 cctcgtggag atcgcgggtc ttttcttcta ctgacgcctc cagagaaagg tacaatttat
    43441 gcagctcgct cgacatctgg ttaaaggttt ttgcaagcag accaagctca ttcggcagac
    43501 tggtatccag cggcggcgag tcgaactgcc cgtgttcaat acgttgactg gcggtaacca
    43561 gctgattcag cggggcaacc acctgatggc gtatacggcg caaagtaaaa aagaccagcg
    43621 tgaaaatacc gataccgcca gccagggaaa tcgccaccac cagcagcatt ttgcgttcag
    43681 cgtagtgctg taaagccagc acgaacaggt ctatctgatt aacataatta ttaatattgg
    43741 cctgatacca cggcaaatcg cccttgctga gccgattatt catctccagc cagttggcat
    43801 tcagatgcgc atagcgagtt tttactgctt ctggcacata ccagacgttg agattggtta
    43861 ataccggtga atgcagtgcc tgctgaaaca actggcgatg tgcattgagt tgtggactgc
    43921 cactttgcaa gtcgtagccc aggcggtaac tctgcatacg tagcgatccg gcaatattga
    43981 tagcctcagc gtcgcgcaaa ctgctcgcca gagttagcag agcgataccc gtggaaagaa
    44041 tcgacagcag cacaatgtaa aaaaaggccc gggccagact ggccgagacg ggtcgtttaa
    44101 caatcacacg cgtcttctcc acaaaaaatc attaatgttc cagttcgctg aaacacaatt
    44161 tagtcagtga tgggattatt tgatctgcca cagtttatag cgaaatagtt acttattctg
    44221 ggcacatgaa cattgccgat tcccctgtaa gtcgggtaat aacaacactc atataaagaa
    44281 taaggttttt acaaccaaaa aagaaggtcg ttatgaatcg ttttattatg gccaacagtc
    44341 agcaatgtct gggttgtcat gcttgtgaaa tcgcctgtgt catggctcac aatgatgagc
    44401 aacatgtcct gagccaacac cattttcatc cccgaattac ggttatcaaa catcaacagc
    44461 aacgtagtgc agtgacctgt caccattgtg aagatgcgcc ctgcgcccgt agctgcccta
    44521 atggcgcaat cagccacgtt gatgacagca ttcaggtcaa tcagcaaaag tgtattggct
    44581 gtaaatcctg cgtggtggcc tgtccgtttg gtacgatgca aatcgtcctg acacccgtcg
    44641 cggcaggaaa agtaaaagcc acggcgcata aatgcgacct ttgtgcaggg cgcgaaaacg
    44701 gtcctgcctg tgttgagaat tgcccggcgg acgcgctgca actggtcact gacgtcgcac
    44761 tctccggcat ggcgaaatcc cgccgcttgc acaccgcacg tcaggagcat caaccgtggc
    44821 atgccagtac cgctgcacaa gaaatgccgg taatgagtaa agtcgaacaa atgcaggcaa
    44881 cgcccgcgcg tggcgagccg gataaactgg cgattgaagc gcgcaaaacc ggttttgatg
    44941 aaatttatct gccatttcgc gccgaccagg cacaacggga agcctcgcgc tgccttaagt
    45001 gcggcgagca cagcgtttgt gaatggacct gcccgctgca taaccatatt ccgcagtgga
    45061 ttgaactggt gaaagccgga aacatcgacg ccgccgtcga gctttctcac cagaccaaca
    45121 ccctgccgga aattaccgga cgcgtttgtc cgcaagaccg tttgtgtgaa ggtgcctgta
    45181 ctattcgcga tgagcacggc gcggtaacta tcggcaacat tgaacgctat atttcagatc
    45241 aggcgttggc gaaaggttgg cgtcctgact taagtcatgt caccaaagtg gacaagcggg
    45301 tggcgattat cggggcaggt ccggcagggc tggcctgtgc ggatgttctg acccgcaatg
    45361 gcgtggcggt gacggtgtac gatcgccatc cagaaatcgg tggcttgctc actttcggca
    45421 ttccttcttt caaactggat aaatccctgc tggcacgccg tcgggaaatc ttcagcgcga
    45481 tggggattca cttcgaactc aattgtgaag tgggtaaaga tgtctctttg gattcgcttt
    45541 tggaacaata cgacgcggtc ttcgttggcg taggcactta ccgttccatg aaagcgggtt
    45601 tacccaatga agatgcgccg ggcgtttatg acgcgctgcc gttcctcatt gccaacacta
    45661 aacaggtgat ggggctcgaa gagctaccgg aagagccgtt tatcaatacc gccggactta
    45721 acgtcgtggt actgggcggc ggcgacaccg cgatggactg tgtgcgtact gcgctgcgcc
    45781 acggcgcgag taacgtcacc tgcgcttatc gtcgtgatga agctaacatg ccaggctcga
    45841 agaaagaggt gaggaacgcc cgcgaagagg gggccaactt cgaatttaac gtccagccgg
    45901 tggcgcttga gctgaatgaa caaggtcacg tctgcgggat tcgtttcctg cgcacgcgtc
    45961 ttggcgagcc ggatgcccag gggcgtcggc gacctgtacc gattgagggc agtgaatttg
    46021 tcatgccagc cgatgcggtg attatggcgt ttggcttcaa tccacacggg atgccgtggc
    46081 tggagtcgca cggtgtaacg gtagacaaat ggggccgcat catcgcggat gtggaaagcc
    46141 agtaccgtta ccagaccacc aatccgaaaa tcttcgccgg tggcgacgcc gtgcgtggtg
    46201 cggatctggt ggttaccgca atggcagaag gacgtcatgc ggcacagggg attattgact
    46261 ggctgggggt aaaatcagtc aaatctcact gatagcctgc gcagacaaac ccgacttcac
    46321 agcgtaatat aattgttcat ttcgcgctgt ggagtcggta tgacgcaaca aatcaccctc
    46381 attaaagaca aaattctctc cgataactat ttcaccctgc acaacattac ttacgatctc
    46441 acgcgcaaag atggcgaagt tatccgccat aaacgtgaag tttacgatcg cggtaatggt
    46501 gcgacgatcc tcctgtacaa cgcgaagaaa aagaccgtgg ttctgattcg tcagttccgt
    46561 gtcgctacct gggttaatgg caatgaaagc gggcagctga ttgaaacctg cgccgggctg
    46621 ctggataacg acgaaccgga agtgtgcatt cgtaaagaag cgattgaaga gacgggctat
    46681 gaagtgggcg aagtgcgcaa attatttgaa ctgtatatgt cgcctggcgg tgtgactgag
    46741 ctaatccact tttttatcgc cgaatacagt gacaatcagc gcgctaacgc cggtggaggt
    46801 gttgaagatg aagatattga agtgctcgag ctgccgttca gccaggcgct ggagatgatc
    46861 aaaaccggcg agatacgtga cggtaagacg gtgttattgc ttaactattt gcaaacgtca
    46921 catttaatgg actgaaaaat aacaataata tttcgttgtt tattattggc tcaatccgac
    46981 aaatctgatt gagccgcgct acctgcgcaa cgaagatacg acttgtgctg tttgtttgaa
    47041 cttctggggt cgtaccgtcc atgcgctatc gcattttcct tctctttttt ttcgctttgt
    47101 tgccgacgtc tttggtgtgg gcggcaccag cgcaacgggc gttttccgac tggcaggtca
    47161 cctgtaataa ccaaaacttc tgcgtggcgc gcaatacggg cgatcataat ggactggtga
    47221 tgaccctgag ccgcagcgcc ggggcgcata ccgatgccgt tttacgtatt gagcgcggcg
    47281 ggttgaagtc gccggatgcg tctgaaggag agatagcgcc acggctgctg ttagatggtg
    47341 agccgttggc gttaagtggt gacaagtggc ggatttcacc atggttatta gtaaccgatg
    47401 atacggcaac catcaccgcg tttttgcaga tgattcagga agggaaggcg atcaccttac
    47461 gcgacggcaa tcagaccatt tctctgagtg gcttaaaagc ggcgttgttg tttattgatg
    47521 ctcagcaaaa gcgcgttggc agtgaaaccg cgtggatcaa gaaaggggac gaaccgccgc
    47581 tcagcgtacc gcccgcgcct gcgctgaaag aggtcgcggt ggttaaccca acgccgacgc
    47641 cactctcact cgaagaacgc aacgatttgc tggattatgg aaactggcgg atgaatggtc
    47701 tgcgctgctc gcttgatccg ttacgtcgtg aggtgaatgt cactgcgctg actgatgata
    47761 aagcgctgat gatgattagc tgtgaggcag gggcctataa caccattgat ttggcatgga
    47821 ttgtgtcgcg taaaaagcca ctagcctcgc gcccggttcg gttgcgtttg ccgttcaaca
    47881 acggtcagga gacgaatgaa ctggaactga tgaacgcaac atttgatgag aaatcgcgtg
    47941 aactggtgac cttagcgaaa gggcgcggat taagcgattg tggcattcag gcgcgctggc
    48001 gctttgacgg tcagcgtttt cgcctggtgc gttatgccgc agaacccacc tgcgataact
    48061 ggcatgggcc agacgcctgg cccacgttgt ggatcacccg ttaaacaaaa ttgcggggac
    48121 atgtaggccg gataagacgc gtcagcgtcg catccggcaa tcagcatccg gcgatcacca
    48181 tcaggcacct ttcactccca gcaccttatg cgcttttgcc acaatattct cggcggtaaa
    48241 gccaaagaac gggaacagct tatccgccgg agcagattcc ccgtaacccg tcatcccgac
    48301 aattgcccct ttcagaccaa catacttgta ccagtaatcg gcaatacctg cttccaccgc
    48361 cacgcgagcc gcaacgttag aaggcaacac cgactcccga tattcctcat cctgggcgtc
    48421 gaaaatatcg gtcgagggca gggaaactac gcgtacattg cgaccttctc ctgctaattt
    48481 ctctgccgct tgcagggtaa tttccatctc tgaaccggtg gcaatcagaa taatatctgg
    48541 cttaccgccg ctgtctttca gcacataacc gccacgagca atctctttaa cctgatccgg
    48601 cgtacgttcc acctgggcca gattctgcct tgagaggatc agtgccgtcg gtccgttgtg
    48661 gcgctcaacc gccagcttcc agcccaccgc cgcttccacc tgatcgcacg gtcgccaggt
    48721 gctgaaattt ggcgttaagc gcaggctggc cagttgctca acagcctggt gcgtcggacc
    48781 atcttcgccc aggccaattg agtcgtgggt ataaaccata atctgccgcg ctttcatcag
    48841 tgccgccatc cgcgcggcgt tacgggcgta ttcaacaaac atcaggaacg tcgcggtata
    48901 cggcacaaag ccgccgtggt gcgcgatgcc gttggcgata gcggtcatgc caaattcacg
    48961 caccccgtag tgaatgtagt tgcccgctgg atcttccttc agcgaaacag aacctttcca
    49021 gatggtcagg ttgctgggag ccagatccgc cgaaccgccg agcaactcag gcagcatcgg
    49081 cccgtaagcg ttaagcgtat tttgcgaagc cttacgggta gcgattttcg ccggatttgc
    49141 ctgtaactca ttgatatatt tctgagtcgt tttctcccag tccttcggta aaccaccgct
    49201 catccgtcgg gtaaactctt ctgccagttg cggatgagcc tttttatagg cggcaaactt
    49261 ctcattccag ctctgctgcg ctttttcgcc tttttcacgg gcatcccagg cgtgataaat
    49321 ctttttaggg atctcaaatg gcggatggtg ccagcccagt ttttgccgtg ccagcgccac
    49381 ttcttcttcc cccagtggtg cgccgtgcgc ctcttcctta cctgctttat tcggcgaacc
    49441 aaagccaatc accgtacggc agataatcag cgacggctta tctttcacgc tttgcgcttc
    49501 aaggatcgct tccttcaccg cctgcagatc gtgaccgtcg atttcatgga tcacatgcca
    49561 gtgataggct tcaaaacgtt ttgccgtatc gtcggtaaac cagccttctg tttcaccgtc
    49621 gatggaaata ccgttgtgat cgtaaaaacc aatcagcttg cccagtccca gcgtgcctgc
    49681 cagcgaacag acttcgtggg aaataccttc catcaggcag ccgtcgccca taaacacata
    49741 ggtgaagtga tcgacgatct catggtctgg ctggttaaac tgcgccgcca gtgtacgctc
    49801 tgctatcgcc agcccgacgg cgttcgccaa accttgtcca agcgggccgg tggtggtttc
    49861 aacgcctggc gtatagccaa tctccgggtg gcctggggtt ttcgaatgca actgacggaa
    49921 gttcttcagt tcttccagcg gcaggtcgta accggtcaga tgtagcaaac tgtagagcag
    49981 catcgacgcg tgaccgttgg aaagaataaa gcggtcgcga tcataccagg ttgggtcggt
    50041 agggttatgt ttaagaaaat cgttccacag cacttcggca atatcagcca tgcccatcgg
    50101 cgcgccggga tgaccagagt tggctttttg taccgcatcc atactgagtg cgcgaatcgc
    50161 attggcaagg tcttttcggg acatataaca ctccgtggct ggtttatagt ttggcggcaa
    50221 gaagatcttc cagtttgcgt tgatcaacgg cgaacagacg aatgccttca gacagttttt
    50281 ctaccgccat cgcatcctga ttgtgctccc aacggaactc cgcttcgctc atgggagctg
    50341 ggcgtgggaa cgtctgagaa ggtgggatta atttacgtac cactggcgaa actttttcct
    50401 gcagctcctt cagcaaattc ggtgcgatag tcagtcgatc gcagccggtt aaggcgagga
    50461 tttgttcggt gcgacggaag ctcgcgccca tcacaatggt ttcatagtgg tgttgcttat
    50521 agtagtcgta gatattgcgc accgatttaa cgcccggatc ttcttccacc acatacgggt
    50581 ccatcggctt gcgtgcctga taccagtcat aaatacgccc gacaaacggc gaaatcagaa
    50641 aaacgcctgc ttccgcacag gcccgtgcct gtgcaaaaga aaacagcagc gtcaggttgc
    50701 agttaatacc ttctttttcc agctcttctg ccgcgcgaat tccttcccag gtcgaagcca
    50761 gcttgatcag aatgcgtgat ttctcaacgc cttgttgctg atacaagtcc accagatggc
    50821 gcgccttctc aatactcttt tctttatcaa aagagaggcg tgcatcaact tctgttgaca
    50881 cgcgaccggg tacgattttg aggatttcag caccgaaatt gaccgccagt ttgtcacacg
    50941 ctgcgaccac ctgttgttcc tgggtcttgc catttttttt accccaggcg atagcatcgt
    51001 ctattaaatg ctcatattgt gataatccgg cagctttgag taacagcgaa ggattggtgg
    51061 tggcatcctg gggatgataa tggcgaatgg actcaatatc gccgctgtct gccacgacag
    51121 tggtgaactg tttgatgccg tctaactcgt tcataggaaa tactccttga aaagtaaagt
    51181 gttagatgag tgcgttaatt cacacttctg agaaatttcg ctaaacgcat caaaaaagca
    51241 tagcagacag gcatggtatt gctggattaa gcaggtaaca tcagtgttat aggattatta
    51301 ccaaaacatt atattaatgg tgagagtttg gacttgctca aagtctgtag actccggcag
    51361 ggtaataatg tgcgccacgt tgtgggcagg ggctgttgcc cacacacttt atttgtgaac
    51421 gttacgtgaa aggaacaacc aaatggatga ccagttaaaa caaagtgcac ttgatttcca
    51481 tgaatttcca gttccaggga aaatccaggt ttctccaacc aagcctctgg caacacagcg
    51541 cgatctggcg ctggcctact caccaggcgt tgccgcacct tgtcttgaaa tcgaaaaaga
    51601 cccgttaaaa gcctacaaat ataccgcccg cggtaacctg gtggcggtga tctctaacgg
    51661 tacggcggtg ctggggttag gcaacattgg cgcgctggct ggcaaaccgg tgatggaagg
    51721 caagggcgtt ctgtttaaga aattcgccgg gattgatgta tttgacattg aagttgatga
    51781 actcgacccg gacaaattta ttgaagttgt cgctgcgctc gaaccgacct tcggcggaat
    51841 caacctcgaa gacattaaag cgccagaatg tttctatatt gaacagaaac tgcgcgagcg
    51901 gatgaatatt ccggtattcc acgacgatca gcacggcacg gcaatcatca gcactgccgc
    51961 catcctcaac ggcttgcgcg tggtggagaa aaacatctcc gacgtgcgga tggtggtttc
    52021 cggcgcgggt gccgcagcaa tcgcctgtat gaacctgctg gtagcgctgg gtctgcaaaa
    52081 acataacatc gtggtttgcg attcaaaagg cgttatctat cagggccgtg agccaaacat
    52141 ggcggaaacc aaagccgcat atgcggtggt ggatgacggc aaacgtaccc tcgatgatgt
    52201 gattgaaggc gcggatattt tcctcggatg ttccggcccg aaagtgctga cccaggaaat
    52261 ggtgaagaaa atggctcgtg cgccaatgat cctggcgctg gcgaacccgg aaccggaaat
    52321 tctgccgccg ttggcgaaag aagtgcgtcc ggatgccatc atttgcaccg gtcgttctga
    52381 ctatccaaac caggtcaaca acgtcctttg cttcccgttt atcttccgtg gcgcgctgga
    52441 cgttggcgca acggctatca atgaagagat gaaactggcg gcggtacgcg cgattgcgga
    52501 actggcccac gccgaacaga gcgaagtggt ggcttcagcg tatggcgatc aggatctgag
    52561 ctttggtccg gaatacatca ttccaaaacc gtttgatccg cgcttgatcg ttaagatcgc
    52621 tcctgcggtc gctaaagccg cgatggagtc gggcgtggcg acgcgtccga ttgctgattt
    52681 cgacgtctac atcgacaagc tgactgagtt cgtttacaaa accaacctgt ttatgaagcc
    52741 gattttctcc caggctcgca aagcgccgaa gcgcgttgtt ctgccagaag gggaagaggc
    52801 gcgcgttctg catgccactc aggaactggt aacgctggga ctggcgaaac cgatccttat
    52861 cggtcgtccg aacgtgatcg aaatgcgcat tcagaaactg ggcttgcaga tcaaagcggg
    52921 cgttgatttt gagatcgtca ataacgaatc cgatccgcgc tttaaagagt actggaccga
    52981 atacttccag atcatgaagc gtcgcggtgt cactcaggaa caggcgcagc gggcgctgat
    53041 cagtaacccg acagtgatcg gcgcgatcat ggttcagcgt ggcgaagccg atgcaatgat
    53101 ctgcggtacg gtgggcgatt atcatgaaca ttttagcgtg gtgaaaaatg tctttggtta
    53161 tcgcgatggc gttcacaccg caggtgcaat gaacgcgctg ctgctgccga gtggtaacac
    53221 ctttattgcc gatacctacg tcaatgacga accagacgca gaagagctgg cggagatcac
    53281 cttgatggcg gcagaaactg tccgtcgttt tggtattgag ccgcgcgttg ctctgttgtc
    53341 gcactccaac tttggttctt ctgactgccc gtcgtcgagc aaaatgcgtc aggcgctgga
    53401 actggtcagg gatcgtgcgc cagatctgat gattgatggt gaaatgcacg gcgatgcagc
    53461 gctggtggaa gcgattcgca acgaccgtat gccggacagc cctttgaaag gttctgccaa
    53521 tattctggtg atgccgaaca tggaagctgc ccgcattagt tacaacttac tgcgcgtttc
    53581 cagctcggaa ggtgtgactg tcggtccggt gctgatgggc gtggcgaaac cggttcacgt
    53641 gttaacgccg atcgcatcgg tgcgtcgtat cgtcaacatg gtggcgctgg ccgtggttga
    53701 agcgcaaacc caaccgctgt aattcttttt taactctcac gcttaccctg aatattcagg
    53761 gtaagcggtt tggctgcaat atattagtaa agcttattac tgagtttgcg aataataaaa
    53821 aaaagcagtc tatataatat ctcgatatta ttttatttat attcatgcgt tgcatatgaa
    53881 agtttatgca ccacagcgaa tatctctcca ttcttagtga tctacctcac cttttaaacg
    53941 cgcttgccga attttgttat ttactctgac gaaaaattgt cacgatacac gaaagttttt
    54001 cacaggcggc gactcatgga taaagaacgc atcattcagg aatttgtgcc cggcaaacag
    54061 gtcacgctgg cgcatctcat cgcgcatccc ggcgaggaac tggcgaagaa aatcggcgtt
    54121 ccggatgcgg gcgcaattgg cattatgacg ctaactcccg gcgaaacggc gatgatcgcc
    54181 ggtgatttag cgctgaaagc cgctgatgtg catatcggtt ttctcgaccg cttcagcggt
    54241 gcattggtga tttatggctc ggtgggcgca gtagaagaag cgttatcaca aaccgtcagc
    54301 ggtttaggtc gcttattaaa ttatacgctt tgtgaaatga ccaaaagtta atacgaggca
    54361 ttcatgaaac gaattgcgtt tgtgggtagc gtcggggcag gaaaaacaac gttatttaat
    54421 gcgttgcagg ggaattatac cctcgccaga aaaacacagg ccgtggaatt taatgataag
    54481 ggcgatattg atacgccggg tgaatatttt agccatcccc gctggtatca cgccttaatt
    54541 accacgctac aggatgtaga catgctgatc tatgtccatg gcgcaaatga cccggaaagt
    54601 cggttgcctg ccgggttgct ggatattggc gtcagcaaac ggcaaatcgc cgtcatcagt
    54661 aaaacggaca tgccagatgc tgacgtcgcc gcaacgcgaa agttattgct tgagacgggt
    54721 ttcgaagagc cgattttcga gctaaacagc cacgacccgc aaagcgtgca gcaactggtg
    54781 gattatctgg catctctcac caaacaggag gaagcaggtg aaaaaactca tcacagcgaa
    54841 tgatattcgt gaagcacacg cgcgcggcga acaggcaatg tccgtggttc tgcgcgccag
    54901 cattattacc ccggaagccc gtgaagtggc ggatttactg ggttttacca tcaccgaatg
    54961 tgatgaatcg atcccggtaa cggcgtccgt gcccgccagc gtgccagccg ataaaaccga
    55021 aagccagcgc atccgcgaaa ccatcattgc ccagctgccg gaaggccagt ttaccgaaag
    55081 cctggtcgcg cagctgatgg aaaaagtgat gaaggaaaaa cagtcgctgg agcagggcgc
    55141 aatgctgccg agcttcaaat cggtgaccgg caaaggcggc atcaaagtga ttgatggcag
    55201 cagcgtcaag tttggtcgtt ttgatggcgc acaaccgcat tgcgtgggct tgactgatct
    55261 tgtgatcgga gacgacggca gcagcatggc cgccggtttt atgcagtggg agaacgcatt
    55321 cttcccgtgg accctgaact acgacgaaat cgacatggtg ctggaagggg agctccatgt
    55381 tcgccacgaa ggcgaaacca tgatcgccaa agcaggcgac gtgatgttta tcccgaaagg
    55441 ctccagcatc gaatttggta cgacatccag cgtgaaattc ctgtatgtcg cctggccggc
    55501 taactggcaa tccctatgaa agatttcatc accgaagcat ggctaagagc gaaccatacg
    55561 ctcagcgaag gcgtagagat ccatctccct gcggacagtc gcctgacgcc ctctgcccgg
    55621 gagttgctgg aaagccgcca tctgcgcatc aagtttattg acgagcaggg ccgcctgttt
    55681 gttgacgatg aacagcagca gccgcagccc gttcatgggc tgaccagtag cgatgaacat
    55741 ccgcaggcgt gctgcgaact gtgccgccag ccggtggcaa aaaagccgga tacgctgact
    55801 cacctgtcgg cggaaaaaat ggtcgccaaa agcgatccgc gtctgggttt tcgcgcggtt
    55861 ctcgacagca ccattgcgct ggcagtgtgg ctgcaaattg aactggcgga accgtggcag
    55921 ccgtggctgg cggatatccg ttcgcgtctt ggcaacatta tgcgcgccga tgcgctgggg
    55981 gaaccgctgg gctgccaggc gattgtcggg ctttctgacg aagatctgca tcgactttct
    56041 caccagccgc tgcgctatct cgaccacgat catctggtgc cggaagccag ccacgggcgc
    56101 gatgcggcgt tgctcaatct gctacgtacc aaagtgcggg aaaccgaaac cgtagcggcg
    56161 caggtgttta tcacccgcag ctttgaagta ttacgcccgg acattttgca ggcgctgaac
    56221 cgcctttcca gcacggtcta cgtgatgatg atcctgagcg taacgaagca gccgctaacg
    56281 gtgaagcaga tccaacaacg actgggggaa acgcaatgat tattgaacgt tgtcgtgaac
    56341 tggcgttgcg agcgcccgcc agagtggttt ttccggatgc gttagaccaa cgtgtgctga
    56401 aagccgcgca atatttacat caacaaggtc tggcaacgcc cattctggtc gccaatccgt
    56461 ttgaacttcg tcagtttgcg ctcagtcacg gcgtggcgat ggacgggcta caggtgattg
    56521 atccgcatgg caacctcgca atgcgggaag aatttgctca tcgctggctg gcccgcgcgg
    56581 gcgaaaaaac gccgccggat gcgctggaaa aacttaccga ccctctgatg tttgccgcag
    56641 caatggtcag cgccggtaaa gcggatgtct gtatcgcggg caacctctct tccacggcaa
    56701 atgtgctgcg tgccggatta cgcattatcg gcttgcagcc aggctgtaaa acgctctctt
    56761 ccattttcct gatgttgcca cagtacagcg gcccggcgtt gggctttgcc gattgcagcg
    56821 tggtgccaca gccgacggcg gcgcagctgg cggatatcgc gcttgccagt gccgaaacct
    56881 ggcgcgccat caccggagaa gagccgcgcg tggcgatgct gtcgttttcc agcaatggta
    56941 gcgcccgtca cccctgtgtt gctaacgtcc agcaggcgac agaaatcgtc cgtgagcgcg
    57001 caccaaagct ggtggtggat ggcgagttac agtttgacgc cgccttcgtg ccggaagtgg
    57061 cggcgcaaaa agcgcctgcc agcccgctac agggcaaggc caatgtgatg gtttttccgt
    57121 cgctggaagc cggaaatatt ggttacaaaa tcgcacaacg actcggcgga tatcgtgccg
    57181 tcgggccact gatacaagga cttgccgcgc cgatgcacga tctctctcgt ggttgtagcg
    57241 tgcaggaaat tatcgagctg gcgctggtgg cagctgtgcc gcgtcagaca gaagtgaacc
    57301 gcgaaagcag tttacaaaca ctggttgaat gaacggtccc gttctggacc ctgataaaga
    57361 ggaaagcaca atggaagcat taggaatgat cgaaacccgg ggcctggttg cactgattga
    57421 ggcttctgac gcgatggtga aagctgcgcg cgtcaagctg gttggtgtta agcagattgg
    57481 cggcggcctg tgtactgcca tggttcgtgg tgatgttgca gcctgtaaag ccgcgaccga
    57541 tgcaggtgcc gcagcggcac agcgtatcgg cgagctggta tctgtacacg ttatcccgcg
    57601 tccgcacggc gacctggaag aagtcttccc gatcggcctg aaaggcgata gcagcaacct
    57661 gtaatcaccc tgtgtgacgt cgattcgggc gtcacgctct ctcctttttc attttacctt
    57721 ctgcggaggg tagggggagc ttttacctga aatacgccac ggaggcgggt atgaaactgg
    57781 cagtcgtcac tggacaaatt gtttgtaccg tacgccatca cggactggcg catgacaaat
    57841 tgctgatggt ggaaatgatt gatccacaag gtaatcctga cgggcaatgc gccgtcgcca
    57901 tcgacaatat tggcgcggga accggggagt gggtgttact ggtgagtggc agttccgccc
    57961 gccaggcgca taaaagcgaa acgtcaccgg tcgatctgtg cgtgattggc attgtcgatg
    58021 aggtggtgtc tggcggtcag gtaattttcc acaaataagg cagaacatca tgaatcaaca
    58081 ggatattgaa caggtggtga aagcggtact gctgaaaatg caaagtagtg acacgccgtc
    58141 cgccgccgtt catgagatgg gcgttttcgc gtccctggat gacgccgttg cggcagccaa
    58201 agtcgcccag caagggttaa aaagcgtggc aatgcgccag ttagccattg ctgccattcg
    58261 tgaagcaggc gaaaaacacg ccagagattt agcggaactt gccgtcagtg aaaccggcat
    58321 ggggcgcgtt gaagataaat ttgcaaaaaa cgtcgctcag gcgcgcggca caccaggcgt
    58381 tgagtgcctc tctccgcaag tgctgactgg cgacaacggc ctgaccctaa ttgaaaacgc
    58441 accctggggc gtggtggctt cggtgacgcc ttccactaac ccggcggcaa ccgtaattaa
    58501 caacgccatc agcctgattg ccgcgggcaa cagcgtcatt tttgccccgc atccggcggc
    58561 gaaaaaagtc tcccagcggg cgattacgct gctcaaccag gcgattgttg ccgcaggtgg
    58621 gccggaaaac ttactggtta ctgtggcaaa tccggatatc gaaaccacgc aacgcttgtt
    58681 caagtttccg ggtatcggcc tgctggtggt aaccggcggc gaagcggtag tagaagcggc
    58741 gcgtaaacac accaataaac gtctgattgc cgcaggcgct ggcaacccgc cggtagtggt
    58801 ggatgaaacc gccgacctcg cccgtgccgc tcagtccatc gtcaaaggcg cttctttcga
    58861 taacaacatc atttgtgccg acgaaaaggt actgattgtt gttgatagcg tagccgatga
    58921 actgatgcgt ctgatggaag gccagcacgt ggtgaaactg accgcagaac aggcgcagca
    58981 gctgcaaccg gtgttgctga aaaatatcga cgagcgcgga aaaggcaccg tcagccgtga
    59041 ctgggttggt cgcgatgcgg cgaaaatcgc ggcggcaatc ggcctgaacg tgccgcaaga
    59101 aacgcgtttg ctgtttgtgg aaaccactgc agaacatccg tttgccgtga ccgaactgat
    59161 gatgccggtg ctgcccgtcg tgcgcgtcgc caacgtggcg gatgccattg cgctggcggt
    59221 gaaactggaa ggcggttgcc accacacggc ggcaatgcac tcgcgcaaca tcgaaaacat
    59281 gaaccagatg gcgaatgcta ttgataccag cattttcgtt aagaacggac cgtgcattgc
    59341 cgggctgggg ctgggcgggg aaggctggac caccatgacc atcaccacgc caaccggtga
    59401 aggggtaacc agcgcgcgta cgtttgtccg tctgcgtcgc tgtgtattag tcgatgcgtt
    59461 tcgcattgtt taaggagtaa cagatggcgc acgacgaaca atggctcacc ccacgtctgc
    59521 aaacggcggc aacgctgtgt aaccagacgc ccgccgcgac ggaatctccg ctgtggctgg
    59581 gggtggatct gggcacctgc gatgtggtgt cgatggttgt cgaccgcgac ggtcagccgg
    59641 tggcggtatg cctcgactgg gccgatgtgg tccgcgacgg catcgtctgg gatttcttcg
    59701 gcgctgtcac cattgttcgt cgccatctcg acacgctcga gcagcaattc ggtcgccgtt
    59761 ttagccatgc ggcgacttca tttcctccag gtacggaccc gcgtatttcc atcaacgtgc
    59821 tggagtctgc tggactggaa gtgagccatg tgctggatga gccaaccgca gttgcggatc
    59881 tgctgcaact ggacaacgcc ggtgtagtgg atatcggcgg cggcactacc ggcattgcca
    59941 tcgtgaaaaa gggcaaagtg acgtactcgg cggatgaagc caccggcgga catcacatct
    60001 ctctgactct cgccggaaat cgccgtattt cgctggaaga ggcggagtag tacaagcgcg
    60061 gtcacggtga agagatttgg cctgcggtga aaccggtgta cgaaaaaatg gcggacattg
    60121 tcgcccggca tattgaaggg cagggcatta ctgatttatg gctggcgggc ggctcctgta
    60181 tgcaaccggg cgtggcagaa ctgtttcgca aacaattccc ggcgttacag gtgcatttac
    60241 cgcagcacag tttgtttacg accccgctgg cgatcgccag tagtgggcga gagaaagcgg
    60301 aggggctata tgcaaaatga attgcagacc gcgctctttc aggcgttcga taccctgaat
    60361 ctgcaacggg taaaaacatt tagcgttcca ccggtgacgc tttgcggtcc gggctcggtg
    60421 agcagttgcg gacagcaagc gcaaacgcgt gggctgaaac atctgttcgt gatggcagac
    60481 agctttttgc atcaggcagg gatgaccgcc gggctgacgc gtagcctggc cgttaaaggt
    60541 atcgccatga cgctctggcc atgtccggtg ggcgaaccgt gcattaccga cgtgtgtgca
    60601 gccgtggcgc agttgcgtga gtcaggttgt gacggggtga tcgcatttgg cggcggctcg
    60661 gtgctggatg cagcaaaagc cgtggcgttg ctggtgacga accccgatag cacgctggcg
    60721 gaaatgtcag aaaccagcgt tctgcaaccg cgcctgccgt tgattgccat tccaacgacc
    60781 gccggaaccg gctctgaaac caccaacgta acggtgatta tcgacgcggt gagcgggcgc
    60841 aagcaggtgt tagcccatgc ttcgctgatg ccggatgtgg cgattctcga cgccgcgttg
    60901 accgaaggtg tgccgtcgca tgtcacggcg atgaccggca ttgatgcgtt aacccatgcc
    60961 attgaagcat acagcgccct gaacgctaca ccgtttaccg acagtctggc gattggtgcc
    61021 attgcgatga ttggcaaatc gctgccgaaa gcggtgggct acggtcacga ccttgccgcg
    61081 cgcgagagca tgttgctggc ttcatgtatg gcgggaatgg tgttttccag tgcgggtctt
    61141 gggttgtgcc acgcgatggc gcatcagccg ggcgcggcgc tgcatattcc acacggtctg
    61201 gcgaacgcca tgttgctgcc aacggtgatg gaatttaacc ggatggtttg tcgtgaacgc
    61261 tttagtcaga ttggtcgggc actgcgaact aaaaaatccg acgatcgtga cgctattaac
    61321 gcggtaagtg agctgattgc ggaagttggg attggtaaac gactgggcga tgttggtgcg
    61381 acatctgcgc attacggcgc atgggcgcag gccgcgctgg aagatatttg tctgcgcagt
    61441 aacccgcgta ccgccagcct ggagcagatt gtcggcctgt acgcagcggc gcaataaatg
    61501 ccggatgcga cgcttgccgc gtcttatcca gcctacggga ttgcacatgt agggcggata
    61561 aggcgttcac gccgcatccg ccagtggcgc ggtgcaattg ccggatgcga tgcttgccgc
    61621 gtcttatccg gcctacggga ttgcacatgt agggcggata aggcgttcac gccgcatccg
    61681 ccagtggcgc cgtgcaattg ccggatgcga cgcttgccgc gtctgatccg gccttcggga
    61741 ttgcacatgt agggcggata aggcgtttac gccgcatccg ccaataaata acaactaaca
    61801 gggagtaaag gcgatgggaa ttaacgaaat catcatgtac atcatgatgt tctttatgct
    61861 gatagctgcc gtagacagga tcctgtcgca gttcggcggt tctgctcgtt tcctcggtaa
    61921 gttcggtaaa agtatcgaag gatcaggcgg tcagttcgaa gaaggcttta tggcaatggg
    61981 cgctctgggt ctggcgatgg tcggtatgac cgcgctggca ccggtactgg ctcacgtact
    62041 cgggccggta attatcccgg tttacgaaat gctcggcgca aacccgtcaa tgttcgccgg
    62101 aacactgctg gcgtgcgata tgggcggctt cttcctcgcc aaagagctgg cgggcggcga
    62161 cgtagcagcg tggctatact ctgggttaat tctcgggtcg atgatggggc caacgattgt
    62221 gttttccatt ccggtggcgc tcggcattat cgaaccttct gatcgtcgtt atctggcgct
    62281 cggcgtgctg gcgggcattg tgaccattcc gattggctgt attgctggtg gtctggttgc
    62341 tatgtactcc ggtgtgcaga tcaacggtca gccggtggaa ttcactttcg ccctgatcct
    62401 gatgaacatg atcccggtga tcattgtcgc gattctggtg gcgctggggc tgaaattcat
    62461 cccggaaaaa atgatcaacg gcttccagat ctttgccaaa ttcctcgttg cattgatcac
    62521 cctcggtctt gccgctgcgg tagtgaaatt cctccttggc tgggaactga tccccggact
    62581 ggatcctatc tttatggccc ctggcgataa acccggtgaa gtgatgcgcg ccattgaagt
    62641 tatcggttct atctcctgcg tgctgttagg ggcgtatcca atggtgctgc tgctgactcg
    62701 ctggtttgaa aaaccgctga tgagcgtcgg taaagtactg aatatgaaca acatcgcggc
    62761 agccggcatg gtggcaacgc ttgccaacaa catcccgatg tttggcatga tgaagcagat
    62821 ggatacccgc ggcaaagtca tcaactgcgc cttcgccgtt tccgctgctt tcgccctggg
    62881 cgaccactta ggcttcgccg ctgccaacat gaacgccatg atcttcccga tgattgtcgg
    62941 caagttgatc ggcggcgtaa cggcgattgg cgtggcgatg atgctggtgc caaaagaaga
    63001 cgcgaccgcg actaaaaccg aagcggaggc acaatcgtga acactcgcca gctattgagc
    63061 gtcggtatcg atatcggcac caccaccacc caggtgattt tctcccacct ggaactggtt
    63121 aaccgtgcgg cggtgtcgca ggtgccgcgc tacgaattca tcaaacgcga aattagctgg
    63181 caaagcccgg tgttctttac ccctgtcgat aaacagggtg gtttaaaaga agcggaactg
    63241 aaatcgctga ttttagagca atatcaggct gcgggcattg cgccggaaag cgttgattct
    63301 ggtgccatca tcatcaccgg tgaaagcgcg aaaacccgca atgctcgccc ggcggtgatg
    63361 gcgctctctc agtcgctggg cgattttgtc gttgccagcg ccgggccgca cctcgaatcc
    63421 gtgatcgccg gtcacggagc cggggcacaa accctttctg aacaacggct gtgtcgggta
    63481 ctgaatatcg acatcggcgg tggcaccgcg aactacgccc tgttcgatgc cggaaaaatc
    63541 agcggcactg cctgcctcaa cgtcggtggt cgcctgctgg aaaccgacag ccaggggcgc
    63601 gtggtttacg ctcataaacc ggggcagatg attgtggatg agtgcttcgg tgcaggcacc
    63661 gacgcccgtt cgctgaccgg cgcgcagctg gtgcaggtga cccgacggat ggcagagctg
    63721 attgtcgaag tgattgacgg aacgctttcg ccgctcgcgc aggcattaat gcaaaccggt
    63781 ttgctgcccg caggtgttac gcccgaaatc attacgcttt ctggcggcgt gggcgaatgt
    63841 tatcgccacc agcccgccga cccgttctgt tttgccgata ttggcccact tctggcaacg
    63901 gcgctgcatg accatccgcg cctgcgtgag atgaacgtgc agtttccggc gcaaaccgtg
    63961 cgcgccacgg tgattggcgc gggcgcgcat accctttcgc tctctggcag cacaatctgg
    64021 cttgagggcg tacaactgcc gctgcgcaat ttgccggtgg cgatcccgat tgatgaaacg
    64081 gatctggtga gtgcctggca acaggctctg cttcagctgg atcttgatcc caaaactgac
    64141 gcgtacgtgc tggcgcttcc cgcctcgctg cctgtgcgtt acgccgcggt actgacggtc
    64201 atcaacgcgc tggtcgattt cgtcgcgcgt tttccgaatc cgcatcccct gctggtggtg
    64261 gccgggcagg actttggtaa agctctgggc atgttgttgc gcccacagct acaacaactc
    64321 ccgttggcag tcattgacga agtgattgtc cgcgcggggg actatatcga cattggtacg
    64381 cctctttttg gcggatcggt tgtgccggtg acggtgaaat cactcgcatt tccttcctga
    64441 gggaacgact tatgaaacta aagaccacat tgttcggcaa tgtatatcag tttaaggatg
    64501 taaaagaggt gctggctaaa gccaacgaac tgcgttcggg ggatgtgctg gcgggcgttg
    64561 cagcggcaag ctcacaggag cgcgtggcgg caaagcaggt gttgtcggaa atgaccgtag
    64621 cggacatccg caataatccg gtgattgcct atgaagatga ctgcgtgacg cggctgattc
    64681 aggacgacgt taacgaaacg gcctacaacc agattaaaaa ctggagcatc agcgaactgc
    64741 gtgagtatgt gctgagcgat gaaaccagcg tggacgacat tgcctttacc cgcaaagggc
    64801 tgacctcgga agtggtcgcg gcggtagcga agatttgctc caacgcggac ctgatctacg
    64861 gcgcgaagaa aatgccggta atcaaaaagg ccaataccac cattggtatt ccgggcacct
    64921 tcagcgcccg tttgcagcca aatgacaccc gtgacgacgt gcaaagtatc gccgcgcaaa
    64981 tctacgaagg gctttccttc ggggtgggcg atgcggtgat cggcgttaac ccggtgactg
    65041 acgacgtgga aaacttaagc cgcgtgttgg acaccattta cggcgtgatc gacaaattca
    65101 acatcccaac tcagggctgc gtactggcgc acgtcaccac ccagatcgaa gcgattcgtc
    65161 gcggcgcgcc tggcggactg attttccaga gtatctgtgg cagcgaaaaa gggctgaaag
    65221 agtttggcgt ggaactggcg atgctcgacg aagcgcgcgc agtgggtgca gagttcaatc
    65281 gtatcgccgg ggaaaactgc ctctacttcg aaaccggaca aggctctgcg ctatccgctg
    65341 gcgctaactt cggcgctgac caggtgacga tggaagcacg taactatggg ctggcgcgtc
    65401 attacgatcc gtttatcgtc aacaccgtgg tcggctttat tgggccggag tatctctaca
    65461 acgaccgcca gattatccgt gctggcttag aagatcactt tatgggcaag ctgagcggca
    65521 tctctatggg ctgtgactgc tgctacacca accacgctga cgctgaccag aacctcaacg
    65581 aaaacctgat gatcctgctc gccaccgcag gctgcaacta catcatgggg atgccgctgg
    65641 gtgatgacat catgctcaac taccagacca ccgcattcca cgataccgcc actgtgcgtc
    65701 agttactcaa cctgcgcccg tcaccagagt ttgaacgctg gctggaaagc atgggcatta
    65761 tggcaaacgg tcgcctgacc aaacgggcgg gcgatccgtc actgttcttc tgatgacgcg
    65821 gagataacac atcatggatc aaaaacagat tgaagaaatt gtacgcagcg tgatggcgtc
    65881 aatgggacaa acggcccccg cgccgtcaga agcaaagtgc accaccacca cctgtgcggc
    65941 accggtgacc tcggaaagct gcgcgctgga tttaggttct gcggaagcaa aagcgtggat
    66001 tggtgttgaa aacccgcatc gcgcagacgt attaacagaa ctgcgtcgca gcaccgtggc
    66061 ccgcgtccgc gtacgcaggc gttgctgcgt ttcctggccg atcactctcg ttcgaaagat
    66121 accgtactga aagaagtgcc ggaagagtgg gtgaaagcgc aggacttgct ggaagtacgc
    66181 tccgaaatta gcgacaaaaa tctctacctg actcgcccgg atatgggccg ccgcctgtgt
    66241 gcagaagctg ttgaagcgtt gaaagcacag tgtgttgcta atccggacgt acaggtcgtt
    66301 atttctgatg gcctgtcaac tgatgccatc accgtgaact acgaagagat cctgccgccg
    66361 ctgatggcgg gcctgaaaca ggccgggctg aaagtcggca cgccattctt tgttcgttat
    66421 ggtcgcgtga agattgaaga tcagatcggt gagatcctcg gcgcgaaagt ggtgatcctg
    66481 ctggtggggg aacgtccggg gttgggtcag tcagaaagcc tctcctgtta cgccgtttac
    66541 tcgccgcgta tggcgaccac cgttgaggcc gatcgcacct gcatctctaa cattcaccag
    66601 ggcggcacgc cgccggttga agccgcagcc gtcattgtgg atttggcgaa acgtatgctg
    66661 gagcagaaag catccggcat caacatgacc cgataaggag gcatcatgcc agctttagat
    66721 ttgattcgac cgtcgataac cgccatgcgg gtgattgcct ccgttaacgc tgaatttgcg
    66781 cgtgaactaa aattgccgcc gcatattcgt agcctcgggc tgatttctgc tgattccgat
    66841 gacgtcacct acattgcggc tgacgaagcg accaagcagg cgatggtcga agtggtatat
    66901 ggccgctcgc tgtatgcggg agcagcacac ggaccgtcac cgactgccgg tgaagtgctg
    66961 attatgcttg gtgggccaaa cctggcggaa gtgcgtgctg gtctggatgc gatggttgcg
    67021 catattgaaa atggcgctgc tttccagtgg gctaatgacg cagaaaacac cgcattcctg
    67081 gcacatgtag tttcgcgtac cggttcttat ctctcatcaa ccgccgggat cacgcttggc
    67141 gatccgatgg cgtatctggt ggcaccgccg ctggaagcga cctacggcat tgatgcagcg
    67201 ttgaaatctg ccgacgtaca gctggtgacc tatgtcccgc caccgtctga aaccaactac
    67261 tcggcagcat ttttaaccgg tagccaggcc gcgtgtaaag cagcctgtaa cgcctttacc
    67321 gatgcagtgc tggaaatcgc gcgtaatcca atccagcgtg cgtaacggag gttgccgatg
    67381 atcaatgcac tgggattgct ggaagtggac ggcatggtgg ccgcgataga tgcagcggat
    67441 gccatgctca aagcagctaa cgttcgtctg ctcagtcacg aagtgcttga ccctggtcgc
    67501 ttaacgctgg tggtggaagg cgatctggcg gcgtgtcgtg cggcgctgga cgcaggttgt
    67561 gctgccgcga tgcgtaccgg gcgtgtcatc agccgcaagg agatcggtcg gccagacgat
    67621 gacacccagt ggctggttac tggctttaac cgccagccga agcaacccgt aaaggaaccc
    67681 gacgcgccag ttatcgtcgc ggaatctgct gacgagttgt tggcgctgtt aacatcagta
    67741 cgtcagggaa tgacggcagg agaagtggct gcccactttg gctggccgct ggaaaaagcc
    67801 agaaatgcgc tcgaacagct cttttctgcc gggacgttac gtaaacgcag tagtcgttat
    67861 cgtctcaagc cccattaacc tgtcggaggt gccgggtgtc ctacaacacc cggcattaac
    67921 atcatgaaaa agacccgtac agccaatttg caccatcttt atcatgaacc cttacccgaa
    67981 aacctgaagc tcacgccgaa ggtcgaagtg gataatgttc atcaacgaca gacaacggat
    68041 gtctatgaac atgctttaac gattaccgcc tggcagcaga tttacgatca gctgcatccg
    68101 ggcaagtttc atggtgaatt tacggaaatt ctactcgatg atattcaggt ttttcgtgaa
    68161 tacaccggtc tggcgctgcg tcagtcgtgc ctggtctggc cgaactcgtt ctggtttggc
    68221 attccggcga cgcgcggtga gcagggattt atcggttcgc aatgtctggg aagcgcggaa
    68281 atcgccaccc gccctggtgg cactgaattt gaactgagca cgccggatga ttacacgatc
    68341 ctgggcgtgg tgctttctga agatgtcatc acccggcagg ctaacttttt gcataacccg
    68401 gatcgggtat tacatatgct gcgtagccag tcggcgctgg aagtgaaaga gcagcataaa
    68461 gccgcgctgt ggggctttgt ccaacaggcg ctggcgacgt tttgcgagaa cccggaaaat
    68521 ctccatcagc ctgcagtgcg aaaagtgctg ggggataatt tgctaatggc gatgggggcg
    68581 atgctggaag acgcgcaacc aatggtgacg gcggaaagca tcagccatca gagctaccgt
    68641 agattacttt cccgcgcccg tgaatatgtg ctggaaaata tgtctgagcc ggtgacggtg
    68701 ctggacttgt gtaatcaact gcatgtcagt cgccgcacgc tacaaaacgc gtttcacgct
    68761 attttaggca ttggcccaaa cgcgtggctg aaacgcattc gcctgaacgc cgtacgccgc
    68821 gaactgataa gcccgtggtc gcaaagcaca acggtaaaag acgccgccat gcagtgggga
    68881 ttctggcatc tggggcaatt tgccacggat taccagcagc tgtttgccga gaagccgtcg
    68941 ttgacgttgc atcagcggat gcgggaatgg gggtgaggga gttacaccca atccctgacc
    69001 ttaataaact cacttaacgc cgcttctggg ctgccatctt ttggctgata atcatactcc
    69061 cagcgtacca gtggcggcat tgacatcagg atcgactcgg tgcgcccgcc agtttgcagg
    69121 ccaaacagag tgccgcgatc ccagaccaga ttgaactcga cataacgacc gcgacggtag
    69181 agctgaaaat tgcgctcgcg ctcgccgtag gccatcgctt tacgtcgctc tacaattggt
    69241 aaataagcgt cggtgtagcc tttgcctacc gcctgcataa aggcaaaaca gtggtcgaaa
    69301 tctggcgtgt tcagatcatc aaagaacagc ccgccaatac cgcgctgttc gttgcgatgt
    69361 ttgaggtaga agtaatcgtc gcaccacttt ttgtaacggg gataaacgtc ttcaccaaat
    69421 ggcaggcaca ggtcacgggc ggtgcggtgc cagtgaatgg cgtcttcttc aaaaccatag
    69481 aaaggggtta aatcgaagcc gccgccaaac caccagacgg gctctgcacc cggtttttcg
    69541 gcaataaaaa aacgcacatt cgcgtggctg gtgggaacat acgggttatg cggatgcact
    69601 accagtgaaa cgcccatcgc ctcgaaactg cgcccggcaa gttccgggcg atgagcggtg
    69661 gcggaagcag gcatcgcctc gccgtggacg tgagaaaagt tgacgcctgc ctgttcgaaa
    69721 acaccaccat tacgcaacac ccgactacgc ccgccgccgc cagcttcgcg ctgccaacta
    69781 tcttcgacaa attctgcgcc atcgacggcg gtcagctgct gacaaatcgt atcctgaagg
    69841 ttgagcagaa actgtttaac ctggtgtgcg tcgggtttca taacttatcg ctttttcgaa
    69901 tgtgctttct ggttgtcgaa ccagtggaaa taactgatca cgccttcagc aatcgctgtg
    69961 gcgattttct gacgaaacgc cgccgtgcct aacagccgct cttcttccgg gttggtgata
    70021 aacgaggttt ccaccagcac cgaaggaacc gacggcgatt tcaacaccac aaacgcggct
    70081 tgttcggtgt tgcggctgtg cagtttatgc accggcttaa tctttttcag aatatgcgag
    70141 ccaagcgtca ggctgttttt aatagtatcg gtttgcacca gatcaaacag cacttgctgc
    70201 aataggtgat ccttgtcagt cgccttttta ccggcaactt catcggcgcg gttttcgcgt
    70261 tcagacaggt atttcgccat tgcgctactt gccccacggt tagagagggc aaataccgaa
    70321 gcaccggcag ctttcgggtt ggtaaagcca tcggcatgaa ttgacataaa cagatctgcg
    70381 ccatgtttat gggcgatttc aacgcgatcg taaagtggga taaacgtatc gccagaacgc
    70441 gttaaacgcg catcaatccc atgattacgc aaaatggaac ggacgttttt agcaatcgcc
    70501 agcaccacat gtttttcttt cgaaccgttg cgtccgatcg ctccggtatc aattccgccg
    70561 tggcctggat cgagaacaac gacacgtttg ccgccagatt ttttggcttt cggcttgctg
    70621 tgtccgttgc tggtttttaa aggttcgtct ttggcgatgg cttgcgacat tcctgacaac
    70681 gtcagggcag ccaaaccggc tttcagcacc tggcggcgcg aagtgagtgt ttttagtggt
    70741 ttaaaagtgc tcatacggcc tgagttgtaa taataaagtt ccagatgtta tatcgtatcg
    70801 cgtaatccgt tacgacggtt tgatgttgag aattgtttta cttttcattt caatagttga
    70861 caactcccca ttatgccact ttttcaaccg attttcgtca gatattggct aattcgaccg
    70921 ttcacgccat aatcagcctt ttaacccgca aaaaggtaac gaataccatg gagatacgcg
    70981 tatttcgcca ggaagatttc gaagaggtca tcaccctttg ggagcgttgc gacttgctgc
    71041 gtccgtggaa cgatccggaa atggacatcg agcgtaagat gaaccatgac gtcagtttgt
    71101 ttttggtcgc tgaggtaaac ggtgaagtgg tcggaacggt gatgggcggt tatgacgggc
    71161 atcgcgggtc tgcttattat cttggcgtgc atccagagtt tcgtgggcgt gggattgcca
    71221 atgcgttgct taatcggctg gagaaaaagc tgattgctcg tggctgcccg aaaattcaga
    71281 tcaatgtgcc ggaagataac gacatggtgc tcggaatgta tgaacgcctg ggatatgaac
    71341 acgccgacgt gctgagtctg ggtaagcgtt tgattgaaga tgaagagtac tgagttccat
    71401 cctgtccatt atgatgcgca cggacgcctg cgtttaccct tgctcttctg gcttgtgcta
    71461 ttgcttcagg cgcgaacctg ggtgctgttt gtcattgccg gtgcgtcgcg cgagcagggt
    71521 actgcgctgc taaatctgtt ttatcccgat cacgataatt tctggctggg gttgattccc
    71581 ggtattccgg cggtgctggc gtttttgttg agcggtcggc gggcttcgtt tcctcgcacc
    71641 tggcatgtgc tctattttct gttgctgttg gcgcaggtgg ttttactttg ctggcaaccg
    71701 tggctgtggc tgaacggtga atccgttagc ggtatcggtc tggcgctggt agtggcggat
    71761 attgtggcgt taatctggct gctgaccaat cgacgtttac gcgcttgttt taatgaagaa
    71821 aaagaataac ggcacttttt ggtgaatttg cactccaagc aacgttattg aataaccaaa
    71881 ggaagtgaca tgaaatcgct gcgtttaatg ttatgcgcta tgccgttgat gctgaccggc
    71941 tgttccacga tgtcgtcagt taactggtcg gccgctaacc cgtggaactg gtttggatcg
    72001 tccaccaaag tgagcgagca gggcgtgggt gaattaacgg cgtccacacc actgcaagaa
    72061 caagccattg ctgatgcgct tgatggcgat tatcgcctgc gcagcggaat gaaaaccgcg
    72121 aacggcaacg tggtgcgctt ttttgaagtg atgaaaggcg acaacgtggc gatggtgatt
    72181 aacggcgatc aaggcacgat cagtcgcatt gacgtgctgg atagcgatat tcctgctgac
    72241 accggtgtta aaatcggtac accgtttagc gacctttaca gcaaagcatt tggcaattgc
    72301 caaaaagctg atggtgatga taatcgtgcc gtcgaatgta aagccgaagg cagtcaacat
    72361 attagctacc agttcagcgg ggaatggcgt ggtcctgaag ggttaatgcc ttcggacgat
    72421 accctgaaaa actggaaagt cagtaaaatt atctggcggc gttaatttgc gtctgaacaa
    72481 accgccgccg cgaaaaaaca cgtaaaatag cgctcaacaa tgccacggat tgcgtggcat
    72541 tcttattttc aggaggaaca atgtctcagg ttcagagtgg cattttgcca gaacattgcc
    72601 gcgcggcgat ttggatcgaa gccaacgtga aaggggaagt tgacgccctg cgtgcggtca
    72661 gtaaaacatt tgccgacaaa ctggcaactt ttgaagcgaa attcccggac gcgcatcttg
    72721 gtgcggtggt tgcctttggt aacaacacct ggcgcgctct gagcggcggc gttggggcag
    72781 aagagctgaa agattttccg ggctacggta aaggccttgc gccgaccacc cagttcgatg
    72841 tgttgatcca cattctttct ctgcgtcacg acgtaaactt ctctgtcgcc caggcggcta
    72901 tggaagcctt tggtgactgc attgaagtga aagaagagat ccacggcttc cgttgggtgg
    72961 aagagcgtga cctgagcggc tttgttgacg gtacagaaaa cccggcgggt gaagagacgc
    73021 gtcgcgaagt ggcggttatc aaagacggcg tggatgccgg cggcagctat gtgtttgtgc
    73081 agcgttggga gcacaacctg aagcagctca accggatgag cgttcacgat caggagatga
    73141 tgatcgggcg caccaaagag gccaacgaag agattgacgg cgatgaacgt ccggaaacct
    73201 ctcacctcac ccgcgttgat ctgaaagaag atggcaaagg gctgaagatt gttcgccaga
    73261 gcctgccgta cggcaccgcc agtggcactc acggtctgta cttctgcgcc tactgcgcgc
    73321 gtctgcataa cattgagcag caactgctga gcatgtttgg cgataccgat ggtaagcgtg
    73381 atgcgatgtt gcgtttcacc aaaccggtaa ccggcggcta ttacttcgcg ccgtcgctgg
    73441 acaagctgat ggcactgtaa gcgtttctga ttcgatgatt aaaggccagc cagtaacaaa
    73501 actgactggc ctttttatta cttttgcttc aacgccgcat acacctgatc aactacccaa
    73561 ccataagttg caatcggtaa ctgaccgctt tcgaacatat tgggattttt ttgtggatcg
    73621 gcaactggcg aatggggctt gttgcttaac atcacaatcg ccatatgatt gaccggatcg
    73681 ataacggtta ctgtcccggt ccagccagtg tgaccgtagg tttgcgggct tgccagcgtg
    73741 ccaaacgtcg gcgtcatggt ggcattacca ttcacgcgcc agccgaggcc aaaagtggca
    73801 tcttccttag agctggtggt gaacatcttc accgtttccg cactgaacag ctgcacatcg
    73861 ccatagcccc cgccgttcag catcgtttgc attaacaccg caatatcgcc ggtattggaa
    73921 aacaaacctg cgtgcccgga aacgccgccc atcgaataaa aggctttttc atcgtgcacc
    73981 tgaccccaga gagtggaggt gcggatattc gggaaatgga tcacgccatc gcgggtattg
    74041 ccgttcaatt ccgtggcggc aatttgttgc ggtttaaagc ctttcagcag cgggttaaat
    74101 accgtatgtg tcaggccgag cgggcgataa atcgactctt caacatagcg gtcaagcggc
    74161 tgaccggtaa ccgactcgac gataaatcca agcagcatat aatcgacatc gctgtagata
    74221 tgtttgctgc cgggctgata ttccagcggc gtgcgcttga tcatctccag cgtctggcct
    74281 ttatcctggg aatataacgc gcccgcgacc gctttattcg ggtattgcgg atctgccggg
    74341 aaaccgccgc tgtgatgcag caggtcagaa atccgcaggg tgtttttgcc tttaatggtg
    74401 tcatttggac tatcggcaaa ccccggaata tatttcgcaa tccgatcgtc aggatgcagt
    74461 ttgccttcgc tcatcagctt ttgcagggcg aagttcgtgg cgtacatttt ggtgtttgag
    74521 gccaggtcat acagcgtccc ggtggtggct ttgacaggct gttccatcaa cacgctgcca
    74581 tcgtactttt tcgccgctcc ccaggctttg cgatacacaa tctgattatc tttaatgatc
    74641 agcaggttta cgccgggata accggcatca acttgctggc taatccagcg atccatctgg
    74701 ttaagccgtt cgacgttaaa ccctgctttc tctggcgagc tttctgtcag aacagggtat
    74761 tttgcggcgc tgacgctaca actgacagcc agcagagaaa gatagagcat tgtccgtttc
    74821 ataatcagtc cagattgacg tttcggcagc caaagagcgt ggtaaaaata aacccgcaaa
    74881 cccatgccac cagaatccca cctgcatata ccgccattgc cggaaggatg ccttgcgcgg
    74941 aagtcatcag cggcagcgcc accagaccag acggcccgaa tgcgctgttt aagcccatcg
    75001 gcagacccca ccaggctatc aatccgataa acaaaccgcc cgctgcgccg cctaaacagg
    75061 cggtaataaa cggtttcatg cggggcaggg tgacaccgta aatcagcggt tcgccaacgc
    75121 ccagcagacc gggaataatc gccccgcgta cctgactgcg taatgcactg tgcggttgcg
    75181 cccgccagta gagtgccagc gccgcgccca cctggcccgc gcctgccatt gaaaggatgg
    75241 gaaataagct gttgaatccc tgactgtcca ttaacgcgag gtaaacagga ataaagccct
    75301 gatgcacgcc aaataccacg gcgatcagga acagcccggc taaaaccgca caaccgagcg
    75361 gattactgtt caggtgcata aacagccacg acatgccttc gaataaccag ccacccagcg
    75421 gcattatgat caggtacgcc agcgtagcgg tgatcagcag ggtgattaac gaggtcagca
    75481 gcatgtcgag atcgtccggc ataaagcggc gcaccatgcc ttcaatgcgg gcgcaggccc
    75541 acgcggcaat cagcacgccg ataatattgc cgcgcggatc gatgggcaga ccaaagaaat
    75601 cgtgaaaacc ggcgtagtaa ccggtggtcg ctgcggggtt ataaccgagc aaaaagagcg
    75661 cggcgataat tgcgccattt acgcccgtgc cgccaaatgc ctgggcggcg ttatagccca
    75721 ccagaatcac caggaaagtg aacaaacctt tgctgaacac cttcataaaa ttcagcgcat
    75781 cgggtagtgt tccctgagca tctgccggaa cgtgcatcac cgtggcaatt aacgtcgcta
    75841 tccccagcag cagaccggcg gcaataaaac cggggatcag cggcgtgaag atggtggcga
    75901 atttggcgag aaattgttgc acgccggagg tttgtttggc ttttaactga cgtttgttct
    75961 gagcggcgat ttctgaggca tcctgtaccg gtgcttctcc cagcagctcg ctcatcgctt
    76021 ttgcggcacg atgcgcttta ccaggcccaa aaacgacctg cacctggtcg ctggtcaaaa
    76081 tgacgccctt cacgccttcc agcgttttga tattgggatc aaccagtgaa ctgtcatgta
    76141 cacccagacg caggcgcgtc atacagttac cacaactggc gatatttccc ggtccgccga
    76201 cacgggtaag aatggtgttc agaagttcac tgctgatctc tttggccatg ggttattcct
    76261 tgtctaaaac ctgacgaata aagccgccgt gttgatccag gcgtttttta gcttctgcgg
    76321 catcgagatt tttcagcacc atcacaatgg ccgttttaca gttgcgctcg caagcaatta
    76381 acgccgcttc tgcttgttcg gcattacatc cggtggcgtt tttaacgata ttgacctggc
    76441 gcacatgcag tttttcgttg gtggcgacca catcgaccat caggttgccg aacactttgc
    76501 cggatttaat catcagcccg gtggagagca tattgagcac cagtttctgc gctgtgcctg
    76561 ctttcattcg cgaagaacca gtgaccactt cggcacctac aatcggtgta atggcaaact
    76621 cggctgtagt ggaaaccgcg ctcccgggat tacaggaaat tcccactgtg cggcatccga
    76681 gctggcgtgc atattccagt ccggcaatca catacggcgt gcgaccactg gctgcaatgc
    76741 caaccaccac atcctgcgcg gttaagccga tgtttttcaa atcattaaca ccgccttccc
    76801 ggctatcttc cgcgccttcc accgcgtgct gaatggcata ttcgccgcca gcaatcaaac
    76861 caaccaccag acccggtttc acgccgtagg tgggcggaca ttcgctggca tccagaatcc
    76921 ccagacgacc ggatgttccc gcaccgaggt aaatcagacg accgccgccg cttacctggg
    76981 cgtggataac atcgatcgcc gcggcgatat ccggcaggac gcgctcaacg gcaagcggta
    77041 cggtttgatc ttcatcgttg ataatccggc acatttccag cgttgatacg cggtcaattt
    77101 cagccgaggt ggtgttcgag ccttcagtaa tcatcttttc aagttgcatc ggtggtgacc
    77161 ttactattta cgatcaaata attgcagatg tatggaatca tatatcccga attgacagac
    77221 ttaaaggaat cattgagtct acactcgcag agtgtgaaga gcgtcataag aacttaagcg
    77281 gaatttatgc tgccagccca ggagagtgga agatgttgta cctgacgaaa ataagcaatg
    77341 cggggagtga attcaccgaa aacgagcaaa caatcgctga ctttctgcag gccaacgtga
    77401 gtgagctgca atccgtttct tcacggcaga tggcgaaaca gctcggcatt agccagtcga
    77461 gcatagtgaa atttgcccaa aaactcggcg cgcaaggatt tactgaattg cgtatggcgt
    77521 taataggcga atacagtgcc agccgggaga aaacgaatgc cacggcgctg cacctgcaca
    77581 gttcgattac cagtgatgat tcgctggaag tgattgcccg caaactgaat cgcgaaaaag
    77641 agctggcgct ggagcaaacc tgtgcgttgt tcgactacgc gcggctgcaa aaaatcattg
    77701 aggtcatcag taaagcaccg tttatccaga taacgggcct gggcgggtcg gcgctggtgg
    77761 ggcgcgatct gtcgttcaaa ctgatgaaaa ttggctatcg cgtggcctgt gaagccgaca
    77821 cgcacgtaca agccaccgtt tcccaggcgt tgaaaaaagg cgatgtacaa atcgctatct
    77881 cttacagcgg cagtaaaaaa gagattgtct tgtgcgcaga agcggcacga aagcaggggg
    77941 caacggtgat tgccattact tctctggctg actctccgct gcggcggctg gcgcatttca
    78001 cgctcgatac cgtttccggt gaaaccgaat ggcgtagctc gtcgatgtct acccgcacgg
    78061 cgcaaaattc tgttaccgat ttactgtttg ttggtcttgt gcaactcaat gatgtggagt
    78121 cattgaaaat gattcagcgc agcagtgagc taacacaacg cctgaaatga tgcataaagc
    78181 agcgactgga ttgagatttt cctgaattag tgagctgatc cgcagcaata ttttgtttat
    78241 cctgtatttt cagagggaat ggagtgtaac gctctgtatt aacaaggaga gcattaaaat
    78301 gggtaaactc acgggcaaga cagcactgat tacgggcgca ttgcagggaa ttggcgaagg
    78361 aattgccaga acttttgcgc gtcatggcgc aaacctaatc ttgctggata tctcccctga
    78421 gatcgaaaag ctggcggacg aactgtgtgg tcgtggtcat cgctgtacgg cggttgtcgc
    78481 cgatgtgcgt gacccggcgt cggtagccgc agctatcaaa cgcgcgaagg aaaaagaagg
    78541 gcgcattgat atcctggtga ataacgcagg cgtttgtcgt ctgggcagtt tcctcgatat
    78601 gagcgatgac gatcgcgatt tccatattga catcaatatt aaaggcgtat ggaacgtcac
    78661 gaaggcggtg ctgccggaga tgattgcccg caaagatggt cgcattgtga tgatgtcttc
    78721 agtcactggt gatatggtgg ccgatcctgg cgaaacggcg tacgccttaa cgaaagcggc
    78781 gattgttggc ctgacaaaat cgctggcggt ggagtacgcg cagtctggta ttcgcgttaa
    78841 cgccatttgc ccgggatacg tgcgcacacc aatggcggaa agcattgccc gccagtcgaa
    78901 cccggaagat ccagagtcgg tgctgactga aatggcgaaa gcaatcccga tgcgtcgcct
    78961 cgccgatccg ctggaagtcg gcgaactggc ggccttcctc gcatcggatg aatccagcta
    79021 tttaaccggt acacagaatg tgattgatgg cggcagcaca ctgccggaga cggttagcgt
    79081 cggtatctga ttcacctctg tttcctccct gcatttgtgg ggaggatttc gttttaaacc
    79141 cggttcttat tcccttttca acttccaaat caccaaacgg tatataaaac cgttactcct
    79201 ttcacgtccg ttataaatat gatggctatt agaaagtcat taaatttata agggtgcgca
    79261 atggccgtta acttactgaa aaagaactca ctcgcgctgg tcgcttctct gctgctggcg
    79321 ggccatgtac aggcaacgga actgctgaac agttcttatg acgtctcccg cgagctgttt
    79381 gccgccctga atccgccgtt tgaacagcaa tgggcaaaag ataacggcgg cgacaaactg
    79441 acgataaaac aatctcatgc cgggtcatca aaacaggcgc tggcgatttt gcagggctta
    79501 aaagcagacg ttgtcactta taaccaggtg accgacgtac aaatcctgca cgacaaaggc
    79561 aagctgatcc cggctgactg gcagtcgcgc ctgccgaata atagctcgcc gttctactcc
    79621 accatgggct tcctggtgcg taagggcaac ccgaagaata tccacgactg gaacgacctg
    79681 gtgcgctccg acgtgaagct gattttcccg aacccgaaaa cgtcgggtaa cgcgcgttat
    79741 acctatctgg cggcatgggg cgcagcggat aaagctgacg gtggcgataa agccaaaacc
    79801 gaacagttta tgacccagtt cctgaaaaac gttgaagtgt tcgataccgg cggtcgtggc
    79861 gcaaccacca cttttgccga gcgtggcctg ggcgatgtgc tgattagctt tgaatcggaa
    79921 gtgaacaaca tccgtaaaca gtatgaagca cagggctttg aagtggtgat cccgaaaacc
    79981 aatattctgg cggaattccc ggtggcgtgg gtcgataaaa atgtgcaggc caacggtacg
    80041 gaaaaagccg caaaagccta cctgaactgg ctctacagcc cacaggcgca gaccatcatc
    80101 accgactatt actaccgtgt gaataacccg gaagtcatgg acaaactgaa agataaattc
    80161 ccgcagaccg aactgttccg cgtggaagac aaatttggct catggccgga agtgatgaaa
    80221 acccacttca ccagcggcgg cgagttagac aagctgttag cggcggggcg taagtaatgt
    80281 ttgcagtttc ttcaagacgc gtgctgccgg gttttacttt aagccttggc accagtctgc
    80341 tgtttgtgtg cctgatattg ctgctgccgc tctctgcgct ggtgatgcag ctttctgaga
    80401 tgagctgggc gcagtactgg gaggtgatca ccaacccgca ggtggtcgcg gcctacaaag
    80461 tgacgctgct gtcggcgttt gtggcatcga tttttaacgg cgttttcggt ctgctgatgg
    80521 cgtggatttt aacacgttat cgctttccgg gccgcacgct gcttgatgcg ctgatggatt
    80581 taccctttgc gctgccaacg gcagtcgccg gtttaacgct agcctcgctc ttttctgtaa
    80641 acggttttta cggtgaatgg ctggcgaagt ttgatatcaa agtcacctat acctggctgg
    80701 ggattgcggt ggcgatggcc tttaccagca ttccgtttgt ggtacgtacc gtgcagccgg
    80761 tgctggaaga gttaggcccg gaatatgaag aagcggcaga aacgcttggc gcaacgcgct
    80821 ggcagagttt ctgcaaagtg gtgctgccgg aactttctcc ggcgctggtg gcgggcgtgg
    80881 cgctgtcgtt tacccgtagt cttggtgaat ttggcgcggt gatttttatc gccggaaata
    80941 tcgcgtggaa gacggaagtg acgtcgctga tgatttttgt gcgcttacag gagtttgatt
    81001 acccggcagc gagcgcgatt gcttcggtga tcctcgcggc atctctgctg ctgctgttct
    81061 caattaacac tctgcaaagt cgctttggtc ggcgtgtggt aggtcattaa tggcggaagt
    81121 tacccaattg aagcgttatg acgcgcgccc gattaactgg ggcaaatggt ttctgattgg
    81181 catcgggatg ctggtatcgg cgttcatcct gctggtgccg atgatttata tcttcgtaca
    81241 ggcgttcagc aaagggctga tgccggtttt acagaatctg gccgatccgg acatgctgca
    81301 cgccatctgg ctgacggtga tgatcgcgct gattgccgta ccggtaaacc tggtgttcgg
    81361 cattctgctg gcgtggctgg taacgcgctt taacttcccc ggacgccagt tactgctgac
    81421 gctactggac attccgtttg ccgtatcgcc ggtggttgcc ggtctggtgt atctgctgtt
    81481 ctacggctct aacggtccgc tgggcggctg gctggatgaa cataatttgc agattatgtt
    81541 ctcctggccg ggaatggtgc tggtcaccat ctttgtgacc tgtccttttg tggtgcgcga
    81601 gctggtgccg gtgatgttaa gccagggcag ccaggaagac gaagcggcga ttttgcttgg
    81661 cgcgtctggc tggcagatgt tccgtcgcgt cacattgccg aacattcgct gggcgctgct
    81721 gtatggtgtg gtgctaacca acgcccgcgc gattggcgag tttggtgcgg tgtcggtggt
    81781 ttccggctcg attcgcggcg aaacactttc gctgccgtta cagattgaat tgctggagca
    81841 ggactacaac accgtcggct cctttaccgc cgccgcgctg ttgacgctga tggcgattat
    81901 caccctgttt ttaaagagta tgttgcagtg gcgtctggag aatcaggaaa aacgcgcgca
    81961 gcaggaggag catcatgagc attgagattg ctaatattaa gaagtcgttt ggtcgcaccc
    82021 aggtgctgaa cgatatctca ctggatattc cttctggtca gatggttgcg ctgctggggc
    82081 cgtccggttc cggaaaaacc acgctgctgc gcattattgc cgggctggag catcaaacca
    82141 gcgggcatat tcgcttccac ggtaccgacg tgagccgcct gcacgcacgc gatcgtaaag
    82201 tcggtttcgt gttccagcat tacgcgctgt tccgtcatat gacggtgttc gacaatatcg
    82261 cttttggcct gacggtgctg ccacgtcgcg agcgcccgaa tgccgcagcc atcaaagcga
    82321 aagtgacaaa attgctggag atggtccagc ttgcgcatct ggcggatcgt tatccggcac
    82381 agctttccgg cggccagaaa cagcgcgtgg cgctggcgcg cgcgctggct gttgaaccgc
    82441 aaattctcct gcttgatgaa ccgtttggcg cgctggatgc gcaggtgcgt aaagagctgc
    82501 gtcgctggct gcgtcaactg catgaagagc tgaaattcac cagcgtgttc gtgacccacg
    82561 accaggaaga agcgaccgaa gtagctgatc gtgtagtggt gatgagccag ggcaatatcg
    82621 agcaggctga tgcgccggac caggtatggc gcgaaccagc gacccgattt gtgctcgaat
    82681 ttatgggcga agtgaaccgc ctgcagggaa ccattcgcgg cgggcagttc catgttggcg
    82741 cacatcgctg gccgctgggg tatacgcctg cgtatcaggg gccggtggat ctcttcctgc
    82801 gcccgtggga agtggatatc agccgccgta ccagtctcga ttcgccgctg ccggtacagg
    82861 tactggaagc cagcccgaaa ggtcactaca cccaattagt ggtgcagccg ctggggtggt
    82921 acaacgaacc gctgacggtc gtgatgcatg gcgacgatgc cccgcagcgt ggcgagcgtt
    82981 tattcgttgg tctgcaacat gcgcggctgt ataacggcga cgagcgtatc gaaacccgag
    83041 atgaggaact tgctctcgca caaagcgcct gataggttga gtgaatgtta aacgcccgga
    83101 ggcgcttccc gcgatccggg ctttttaatg gcaaggtttg taacctgtag acctgataag
    83161 acgcgcaagc gtcgcatcag gcaacaccac gtatggatag agatcgtgag tacattagaa
    83221 caaacaatag gcaatacgcc tctggtaaag ttgcagcgaa tggggccgaa taacggcagt
    83281 gaagtctggt tgaaactgga aggcaataac ccggcaggtt cggtgaaaga tcgtgcggca
    83341 ctttcgatga tcgtcgaggc ggaaaagcgc ggggaaatta aaccgggtga tgtgttaatc
    83401 gaagccacca gtggtaacac cggcattgcg ctggcaatga ttgccgcgct taaaggctat
    83461 cgcatgaaat tgctgatgcc cgacaacatg agccaggaac gccgtgcggc gatgcgtgct
    83521 tatggtgcgg aactgattct tgtcaccaaa gagcagggca tggaaggtgc gcgcgatctg
    83581 gcgctggaga tggcgaatcg tggcgaagga aagctgctcg atcagttcaa taatcccgat
    83641 aacccttacg cccattacac cactaccggg ccggaaatct ggcagcaaac cggcgggcgc
    83701 attacccatt ttgtctccag catgggaaca accggcacta tcaccggcgt ttcacgcttt
    83761 atgcgcgaac aatccaaacc ggtgaccatt gtcggcctgc agccggaaga aggcagcagt
    83821 attcccggca ttcgccgctg gcctgcggaa tatctgccgg ggattttcaa cgcttctctg
    83881 gtggatgagg tgctggatat tcatcagcgc gatgcggaaa acaccatgcg cgaactggcg
    83941 gtgcgggaag gaatattctg tggcgtcagc tcaggcggcg cggttgctgg agcactgcgg
    84001 gtggcaaaag ctaaccctgg tgcggtggtg gtagcgatca tctgcgatcg tggcgatcgc
    84061 tacctctcca ccggagtgtt tggggaagag cattttagcc agggggcggg gatttaagat
    84121 tattgcgcag gcaccgtttt atccgtctgc gcaattaacg catgtaaaaa aggctgtgcg
    84181 ttctcatcgc tcttaccggg gatcttcacg atatacggct tcccggtaat cgatgacgac
    84241 gaagctacct tatcaataaa ctgctcggca gtgtcaatgc ggttacgggt attgccgagc
    84301 ttcagacgca ggtgggaaac cgcttcatcg caggtatgtt catcaccgtt gcgcacaaat
    84361 atcaaatcct ttttctgtgc taatccctcc agcatggcgt tgatgcgggc ttcttcatga
    84421 acagttaact tcgcgtaagc ggggagtgtc attagcagcg taatggccag acaaatgatt
    84481 ttcttcactg aattacctta tccttgtttt gtaatagccg atttgactgc cagaatcagg
    84541 taaagtttcg gttggaatac gatgaggatc atcggcaagg agaaaaaatt aaatgagtag
    84601 tttgttgttg tttaacgata agagtagggc gctgcaggcg gatatcgtcg ccgtgcagtc
    84661 gcaggtagtg tacggcagcg taggcaacag cattgcagtg cctgctataa aacagaacgg
    84721 cctgaatgtc tttgccgtgc cgacggtatt gctgagcaat acgccgcatt atgacacttt
    84781 ctacggtggt gcgattccgg atgaatggtt tagcggctat ttgcgtgcgc ttcaggagcg
    84841 tgatgcgctg cgccaacttc gtgctgtaac cacgggctat atgggaacgg catcgcaaat
    84901 caaaatcctt gccgagtggc tgactgcgct acgcaaagac catcctgacc tattgatcat
    84961 ggtcgatccg gtaattggcg atattgatag cggaatttat gtcaaacctg accttcccga
    85021 agcgtatcga caatatttac tgccgctggc gcagggaatt acccccaata tctttgagct
    85081 ggaaatcctg accggtaaaa attgccgcga tctcgacagt gccattgctg ccgcaaaaag
    85141 tctgctttca gacacattaa aatgggtggt gattaccagc gcctccggta atgaagaaaa
    85201 tcaggagatg caggtggtgg tggtcagtgc cgacagcgtg aatgtcattt cccattcacg
    85261 ggtaaaaacc gacctgaaag ggactggcga cctgttttgt gctcagctta tcagtggttt
    85321 gctgaaaggg aaggcattaa acgatgcggt gcaccgtgcg gggttgcgcg tactggaagt
    85381 gatgcgctac acccagcagc atgagagcaa tgaattgatt ttgccgccgc tggcggaagc
    85441 ataaaaaatg gcgccgatgg gcgccatttt tcactgcggc aagaattact tcttgatgcg
    85501 gataaccggg gtttcaccca cggttacgct accggacagt ttgatcagtt ctttgatttc
    85561 gtccatgttg gagataacaa ccggagtcag ggtagacttg gctttctctt ccagcagcgg
    85621 cagatcaaat tcaatgacag tatcgccaac tttcacgcgc tgaccttctt ctgcaatacg
    85681 cttgaagcct tcgcctttca gttcaacggt gtcgataccg aagtggacga acagttcaac
    85741 gccgctatca gattcgatag agaacgcgtg attggtttca aagattttac caatggtacc
    85801 gtctacaggc gcgaccattt tgttacccgt tggtttgata gcaataccat caccaacgat
    85861 tttttccgca aaaacgacat ccggcacgtc ttcgatattg acgatctcgc cagagagcgg
    85921 agcaatgatc tcaatagttc cggtatcctt cttgtcgtcg gaaaccagag atttcagttt
    85981 atcgaacaaa cccatgatct tctcctaagc agtaaattgg gccgcatctc gtggattagc
    86041 agattgtttt ttcttcaatg aacttgttaa ccagcgtcat taactcgtcc gttgtcggtt
    86101 gagcaagagc ctgctctgct aacaccttcg catcttcgaa gttcgtgtta cggataatct
    86161 tcttaatgcg cgggatagaa atggcgctca tagagaattc gtccagaccc atccccagca
    86221 acagaagtgt agcacgttca tcgccagcaa gctcaccaca catgccagtc catttgcctt
    86281 cagcatgaga agcatcaata acttgcttga tcaagttcag cacggacggt gacattggct
    86341 ggtaaaggtg tgaaatcata tcattaccac ggtcaactgc cagagtgtac tgcgttaaat
    86401 cattggtgcc gatactaaag aaatcaactt ctttggctaa atgacgtgca attgttgcgg
    86461 cagccggtgt ttccaccatt acgccgattt caattgactc gtcaaacgct ttaccttcgt
    86521 cgcgcagttc ctgtttgtag atttcgatct ctttgcgcag tgcacgcact tcttcaacag
    86581 agatgatcat cgggaacata atgcgcaatt taccgaaagc cgaggcacgc aggatagcgc
    86641 ggagctgatc gcgcaggatc tctttacgat ccatcgcgat acggatagcg cgccagccga
    86701 ggaacgggtt ctcttctttc gggaagttca tgtatggcag ctctttgtcg ccgccgatgt
    86761 ccatggtacg aacgataacc gcctgcgagc cacacgcttc agccactgct ttgtaagcag
    86821 caaactgttc ttcttcagtg ggcagcgcgt cgcggtccat gaacaggaac tcagtacgat
    86881 acagaccaac gccttcagcg ccgttacgct ctgcaccttc aacgtcacga accgtaccaa
    86941 tgttagcgca tacttctacc tggtgaccgt ccagcgtaat agctggcaga tctttcagtt
    87001 tagcaagctc tgctttttca gaagccactt gctcctgaac agcgcgcatt ttatcaataa
    87061 cttcgttggt tggattgacg taaacctgat tatttacggc atccagaatc agatagtcgt
    87121 catttttcac ctgagaggtg acgctaccgg tacccacgat agcaggcagt tccagagaac
    87181 gcgccatgat agaggtgtgg gaagtacggc cacccgcgtc ggtgatgaaa cccagcacct
    87241 tcttcaggtt cagctgtgcg gtttcggacg gcgtcaggtc agcggcaacc agaatgactt
    87301 catcctgaat ggcgctcagg tcgataatct tcaggcccag gatgttgcgc agcaggcgct
    87361 taccgatatc acgtacgtca gccgcacgtt ctttcaggta ttcatcatcc agctcttcca
    87421 gggcagaagc ctgaccttcg ataacttcat gagcagctgc gtcagctgtc atgtgtttat
    87481 ctttaatcag ggctatgatt tcctgctcca gctcctcatc ttcgagcagc ataatatgcc
    87541 cttcaaagat ggcttctttt tcttcaccga acgtttcacc agctttcgtt ttgatcgttt
    87601 ccagctgggc tgatgccttg gcacgaccgc tcagaaaacg ttcaacttcc tgatcaacct
    87661 ggtcggcaga aatttttttc cggtcaatga caatttcgtc ttctttcaga agcagagctt
    87721 taccgaaagc gatacccggg gatgctaaaa tgcctgaaat cataacccta ccttacttgt
    87781 gactgatttt taaaagaacc cgggaaatta ctcgagttcc gccatcagtt taaccagatg
    87841 ttcaaccgct ttctgctcgt cttcgccttc tgcggagata gtcacaacgg taccttgagt
    87901 caggcccaga gtctgcagtt taaacaggct tttcgcgctg gcgcttttgc cgttggaagt
    87961 cacagtaatt tcagaagtga agcccttagc ttcttttaca aactgggcag cagggcgggt
    88021 gtgcagaccg ttcggagcgg taatggtaac ttcttgctgg aacattgtat ttccccaact
    88081 tataggttta gtgttgtgga actaaagtct agcctggcgg ttcgacttta gcctgtattg
    88141 ttagcgccag tttttaacag acgcgacgca cgaagagcgc aatacagtcc attgctggcg
    88201 ggtgttgacc gctgacgttt cgtacgtcat taaacattat gccgctaaag gaagaattga
    88261 accaaatcat aaaatcgatt cagctttggc ttttcctgta acgattaatt tcgcgcatca
    88321 aaataattag tctggttaaa taccagttcc gacaggctga atcaatgcca ggaggggcga
    88381 aagtttgaag caggccacaa aaaagcacct aaaaaggtgc ttttttacgc atttttaaca
    88441 agctggcatt actgttgcaa ttctttctca gtgaagagat cggcaaacaa tgcggtgctt
    88501 aaataacgct cacccgatga tggtagaata accacaatat tcttgttggt aaagctttca
    88561 tcttcttgta gtttcaacgc cgcggcaaca gctgctccag aagagatacc tgcaagaata
    88621 ccttcttctt ccattagacg acgggcggta gaaatggctt cttcattggt gatgccaatg
    88681 actttatcga ccagcttgag atcgaggtta gccgggataa aaccagcgcc aataccctga
    88741 attttatgcg ggccaggttt aatctcttca cctgccagcg cctgggcgat aactggagaa
    88801 tcggttggct caacggcgac agagataaga tcggtcttgc ctttggtgcc tttaatgtag
    88861 cggctgacgc cagtcagcgt accgccagtc ccaacgccag caataaatac atcaacctga
    88921 ccgtcggtat cttcccagat ctccggaccg gtggtttttt cgtgaatttc agggtttgcc
    88981 ggattgctga attgttgcag cagcaggtat ttctctggat tgctggcgac aatttcttct
    89041 gctttttgga ttgcgccttt catgccttta gcaccttccg tcagcaccag gtttgcacct
    89101 aacgctttca gcagcttgcg gcgttcaata ctcatggttt ctggcatggt cagggtgagt
    89161 ttgtaaccgc gagcggcagc tacataggcc agtgcaatcc cggtattacc gctggtcggt
    89221 tcaaccagtt caacgcctgg tttcagcacg ccgcgctttt cggcatccca aatcatgttg
    89281 gcaccgatac ggcacttaac gctgaagctg gggttacgag attccacctt cgccagaatg
    89341 cgtccgttac cgatgcgatt caggcgaacc agcggcgtgt gaccgatagt cagcgagtta
    89401 tcttcaaaaa tcttactcat ggcctgtcct taactgtatg aaattgggat acaacaggta
    89461 gcatacccgc tcagagaata tgcggaagta aggatttagc atatctatat acagaaggga
    89521 aataatgaca tgcaagatgg aataaggggc ggcataagcc acccctgttt cacacaaacg
    89581 gtttacaaaa taaccggtag attgttaccg ccacatcgcg tgtttatcgc gatagcaatc
    89641 gacccacatc gccgtcgcgc cacaaacggc aacgggcatg atgaacagat taagcagcgg
    89701 gatcatcgta aacaggctgg ttaaagcacc aaactgcata ttggtgattt tgcgtgtgcg
    89761 cagggcggtg cgcatctctt taaacggcac tttgtggtta tcgaaggggt aatcgcaata
    89821 ctggatggct aacatccagg cgctaaacag gaaccacagt accggcgcga cggtttgccc
    89881 aataccaggg atgaagtaaa gaattagtaa tacaattgcg cgcggcagat accaggcaaa
    89941 tttttgccat tcgcgtttca tgattcgcgg cacatctttc atgataccga aaatcccggt
    90001 atctggcggt gtagcgccag tcaatcgtgc ttccagttgt tcagccaata aaccgttaaa
    90061 cggagcggca atccagttag caatcgtgga gaagaaatag ccaaacacta acagcacaga
    90121 gatgacagcc agaggccaca acagataact cagccattgt agccagtccg gaacgtaact
    90181 catgagagtc gggatccaga catcgagctg tgtaaagagc caccagaatg cgccccccat
    90241 caacaaaata ttgaccagca gcggtaaaat aacgaaacgc cgaatcccag gttgcgagac
    90301 gagcttccag ccttgcgcaa aatagtaaaa accgctgcgt ggggcagatg tgaatgatga
    90361 aaccataatc aggatgagct ccttttgacc aatcccagga aaattctgcg tattttaccg
    90421 ggtaattgcg caatggacag ttaggatatg ttcgaaaaaa cagcaaaaag cacgatttca
    90481 tctatctttg tgctgtgaaa gttaatagtg cacttgcact tgaggtaatc ggcaaatact
    90541 cttagtgagt aaatgtttgc cgtggtggca aggtgttaga acaacagaga atataatgat
    90601 gcaggatttg cgtctgatat taatcattgt tggcgcgatc gccataatcg ctttactggt
    90661 acatggtttc tggaccagcc gtaaagaacg atcttctatg ttccgcgatc ggccattaaa
    90721 acgaatgaag tcaaaacgtg acgacgattc ttatgacgag gatgtcgaag atgatgaggg
    90781 cgttggtgag gttcgtgttc accgcgtgaa tcatgccccg gctaacgctc aggagcatga
    90841 ggctgctcgt ccgtcgccgc aacaccagta ccaaccgcct tatgcgtctg cgcagccgcg
    90901 tcaaccggtc cagcagccgc ctgaagcgca ggtaccgccg caacatgctc cgcgtccagc
    90961 gcagccggtg cagcagcctg cctatcagcc gcagcctgaa cagccgttgc agcagccagt
    91021 ttcgccacag gtcgcgccag cgccgcagcc tgtgcattca gcaccgcaac cggcacaaca
    91081 ggctttccag cctgcagaac ccgtagcggc accacagcct gagcctgtag cggaaccggc
    91141 tccagttatg gataaaccga agcgcaaaga agcggtgatt atcatgaacg tcgcggcgca
    91201 tcacggtagc gagctaaacg gtgaactgct tcttaacagc attcaacaag cgggcttcat
    91261 ttttggcgat atgaatattt accatcgtca tcttagcccg gatggcagcg gcccggcgtt
    91321 attcagcctg gcgaatatgg tgaaaccggg aacctttgat cctgaaatga aggatttcac
    91381 tactccgggt gtcactatct ttatgcaggt accgtcttac ggtgacgagc tgcagaactt
    91441 caagctgatg ctgcaatctg cgcagcatat tgccgatgaa gtgggcggtg tcgtgcttga
    91501 cgatcagcgc cgtatgatga ctccgcagaa attgcgcgag taccaggaca tcatccgcga
    91561 agtcaaagac gccaacgcct gatacactta aggcaaatta actcctcttc gaacccccgc
    91621 ttgtcggggg tttttagcat tgatggtgcg atatggaatc aatcgaacaa caactgacag
    91681 aactgcgaac gacgcttcgc catcatgaat atctttatca tgtgatggat gcgccggaaa
    91741 ttcccgacgc tgaatacgac aggctgatgc gcgaactgcg cgagctggaa accaaacatc
    91801 cagaactgat tacgcctgat tcgcctaccc aacgtgtagg tgctgcgccg ctggcggctt
    91861 tcagccagat acgccatgaa gtaccaatgc tgtcactgga taacgttttt gatgaagaaa
    91921 gctttcttgc tttcaacaaa cgtgtgcagg accgtctgaa aaacaacgag aaagtcacct
    91981 ggtgctgtga gctgaagctg gatggccttg ccgtcagtat tctgtatgaa aatggcgttt
    92041 tagtcagtgc cgcgacccgt ggcgatggca ccaccgggga agatatcacg tctaatgtgc
    92101 gtactattcg cgccattccg ctgaagctgc acggagagaa tatcccgacg cgtctggaag
    92161 tgcgtggtga agtgttcctg ccgcaggcgg ggttcgaaaa gattaacgaa gatgcgcgac
    92221 gcacgggcgg gaaagttttt gctaacccac gtaatgcggc agctggttca ctgcgtcagc
    92281 ttgatccgcg tattacagcg aagcgaccgc tcactttttt ctgctatggc gttggtgttc
    92341 tggaaggtgg cgagctgccg gatactcatc ttggccgttt actgcaattt aaaaagtggg
    92401 ggttgccggt cagcgattgg gtaacgcttt gtgaatcggc ggaagaagtg ctggcgttct
    92461 atcacaaagt ggaagaagac cgcccgacgc tgggctttga tatcgacggc gtggtgatta
    92521 aggtcaactc actggaacag caggagcagc ttggctttgt cgcgcgtgcc ccgcgctggg
    92581 cggtagcgtt taaattcccg gcgcaggagc agatgacctt tgtgcgtgac gtcgagtttc
    92641 aggttgggcg tactggcgcg attacgcctg ttgcgcgtct ggaacctgtc catgttgctg
    92701 gcgtgctggt gagtaacgca accttacaca atgtggatga aatcgaacgt cttggtttac
    92761 gcattggcga taaagtggtg attcgccgcg ctggcgacgt gatcccgcag gtggttaacg
    92821 tcgtgctttc tgaacgcccg gaagataccc gtgaggttgt attcccgacg cattgtccgg
    92881 tatgtggttc tgacgttgag cgtgtggaag gtgaagcggt tgcccgctgt accggtggcc
    92941 tgatttgcgg tgcgcagcgt aaagagtcgc tgaaacactt tgtttcccgc cgtgcgatgg
    93001 atgttgacgg aatgggcgac aaaatcatcg atcagctggt tgaaaaagaa tatgtccata
    93061 ctccggcaga tctgttcaaa ctcaccgcag gcaaactgac cggactggag cgtatggggc
    93121 caaaatcggc acaaaacgtg gttaacgcgc tggaaaaagc gaaagaaacc acctttgctc
    93181 gcttcctcta tgcacttggc atccgtgaag tcggcgaggc caccgcagca ggtctggcgg
    93241 catatttcgg cacgctggaa gcgctggaag ccgcttcgat tgaagagctg caaaaggtgc
    93301 ctgatgttgg cattgtcgtt gcatcccacg ttcacaactt ctttgccgaa gaaagcaacc
    93361 gcaatgtcat cagcgagctg ttggcggaag gtgttcactg gcctgcgccg atcgttatca
    93421 acgcggaaga gattgacagc ccgtttgctg gtaaaaccgt ggtgcttacg ggcagcttaa
    93481 gccagatgtc gcgtgatgac gctaaagctc gactggttga actgggcgcg aaagtcgcgg
    93541 gcagcgtgtc gaagaaaacc gatctggtga ttgccggaga agcggcaggt tctaaactgg
    93601 cgaaagcgca ggaactgggc attgaagtca tcgacgaagc ggaaatgctg cgtttgctgg
    93661 gtagctgaga tggaaaaaga gcagctgatt gaaatagcca atacgataat gccgtttggc
    93721 aaatataaag ggcgtcgctt aatcgacctg ccggaggagt atctgctgtg gtttgcccgc
    93781 aaagatgaat tcccggcagg gaagctcggt gagctaatgc aaatcacgct gctgattaaa
    93841 accgaggggc tgacgcaact ggtccagccc ctgaagcgtc cgctttaagc tttatcggcg
    93901 ctgctttcct gctgtgcctg taactgttcg gtctggcgtt tgtatcgacg cgccagcacc
    93961 gcacagacca tcaattggat ctgatggaaa atcatcaggg gcagcaccat cataccgatc
    94021 accgatgtgg ggaacagaat gtttgccatc gggatgccat ttgccagact ctttttcgaa
    94081 ccacaaaaga cgatagtaat ttcatctgcc ttattgaagc ccagtcggcg tgccataaag
    94141 acgttaacta caatcacgat agccagaaga acgcagctga ccaccacgat aaacagcaat
    94201 aatccccagc caactttatg ccagatacca ttaacgacgg cttcgctgaa cgctgtataa
    94261 accaccaaca gaatggacgt ctggtcagtt ttcgcaatcc attttttatt gcgcgacacc
    94321 cagtcaccaa tccacggccg ggaaagatgc cccaacacaa acggcagcag cagttgcagc
    94381 ataattttac cgacctgctc aaggctgccc cctgcaccgt gaacattcat caccagacca
    94441 accagcaatg gtgaaaggaa aatccccagc aggctggatg ctgacgcaga acaaaccgcc
    94501 gccgcgacgt taccgcccgc cattgacgtg aaggcgattg cagactgcac ggtagccggg
    94561 agaatgcaca agtagagaaa accggagtag agcatcgggt cgacatttac cggtttccac
    94621 caggcaaaca gtacacccag aatcggaaac agcacgaagg tgctgcacat tacccacaaa
    94681 tgcagtcgcc agtgaccacc gccagcaata atcgcctcac gcgacaactt cgcgccgtgc
    94741 ataaagaaca gcagggcaat agctgcggtg gtcagatttt caaagaaggg gacgaaatcg
    94801 cctctggccg gaaagaaaga ggccagcaac accaccgtga tcagggttaa ggtgaaagga
    94861 tcgaggatac gaaaaagttt cataaagact ccagatgatc gatggttcta ttgtgcttct
    94921 tccagtttga gaaataaaat tgatttattg catccattca tgaagaaaac agatgaatta
    94981 ttctttaaaa caattaaagg ttttcgtcac agtagcgcag gagaaaagtt ttagtcgtgc
    95041 aggagagcgt atcggcttga gccagtcggc agtaagtcac agtgtgaagg agctggaaaa
    95101 tcatactggt gttcgcctgc tggacagaac cacgcgtgaa gtggtgctta cagatgcagg
    95161 gcagcagttg gctttgcgtc ttgagcgact gctggatgaa cttaacagca cgttgcgcga
    95221 taccgggcgt atggggcaac aactgagcgg aaaagttcgg gtcgctgcca gccagaccat
    95281 ttccgcgcat cttattccgc aatgcattgc tgaaagccat cgccgctatc cagatattca
    95341 gtttgtcctg cacgatcgtc cgcagcagtg ggtgatggaa agtattcgcc agggagatgt
    95401 cgactttggc atcgtcatcg atccaggccc agtgggcgat ctgcaatgtg aagcgattct
    95461 ttccgagcct ttctttctgc tttgccatcg cgatagcgct ttggccgtgg aagattatgt
    95521 accctggcag gcattgcagg gagcgaaatt ggtgttgcag gattacgcgt caggcagccg
    95581 accgctgatt gacgcagcgc tggcgcgcaa tggtattcag gcgaatattg tgcaggagat
    95641 tggacatccg gcgacgctgt tcccgatggt agccgcaggc atcggcatca gtattcttcc
    95701 cgcactggcg ctaccattgc ctgaaggtag cccactggtg gtaaagcgca ttacgccggt
    95761 tgttgaacgc cagttgatgc tggtgcgcag gaagaaccgt tcactttcca ccgccgctga
    95821 agcgttatgg gacgtggtac gagaccaggg caatgcgcta atggctggtc gggaagggga
    95881 tccgttgtat cagatataga tatcgatatg agtcgttttt tcaacaggat ttagaaggga
    95941 tacattcaga gacaaacact gctctttctc ctgcgccttt tctgcctgct gtttctgcaa
    96001 ctgcgccagt tgtgtttcca gtagggtgat ttgctgttga atcaattcgg cctgtttttg
    96061 cttgtcttca gcactgccgc tagtgtctac aatctcttta atttgttgag tgagtttgat
    96121 gatttgctga cttatccgcg cgatttgtga agcaacatcg ctaccttgtg atgccctgtt
    96181 actactctgt gtttgtatgg aaggaattga agttgaagag atagtggcca ttgttcagtc
    96241 ctcgtcactg tacgaggtaa tatcggtaga agaacaagga acttgagaag aaaaatcaat
    96301 cttacaaatc gcagatagca aaaaagcgcc tttagggcgc ttttttacat tggtgggtcg
    96361 tgcaggattc gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa
    96421 cgacccgaag tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg
    96481 tgctctccca gctgagctaa tcacccactt cggtacttca catttttaca agaaatttag
    96541 ctggcgagaa agtggtgggt gatgacggga tcgaaccgcc gaccccctcc ttgtaaggga
    96601 ggtgctctcc cagctgagct aatcacccac ttctcaattt cttgctacac ggcggagact
    96661 acataaagta gttggtgggt gatgacggga tcgaaccgcc gaccccctcc ttgtaaggga
    96721 ggtgctctcc cagctgagct aatcaccccc gctgtgtgga gtcgcattat agggagagtt
    96781 caaaatgagt caacgcattt tctaaagaaa ttgttcgttc gtcgtaaatt taagcaagat
    96841 gatcgcaaaa cagaccgtgt tgcgcaattt gtcaacgaaa acaataatgc gtaaggtaga
    96901 aacccgaact acattgagga atcaggcggg agtgatagaa tatcgcccac tcaatttttc
    96961 caggatttgc cggttgtcgg catctttcta aacgtaaggc catttcatga aaatcaaaac
    97021 tcgcttcgcg ccaagcccaa caggctatct gcacgttggc ggcgcgcgta ctgctcttta
    97081 ctcctggctt tttgcacgta accacggcgg tgagttcgtg ctgcgtattg aagacaccga
    97141 tcttgagcgt tccacgccgg aagctatcga agccattatg gatggcatga actggctgag
    97201 cctagagtgg gatgaaggtc cgtactacca gaccaaacgt tttgatcgct acaacgcggt
    97261 gatcgatcag atgctggaag agggcactgc ttataaatgc tattgctcta aagagcgcct
    97321 ggaagcgctg cgcgaagagc aaatggcgaa aggtgagaag ccgcgttatg acggtcgctg
    97381 ccgccacagc catgagcatc acgctgatga tgaaccgtgc gttgtacgtt ttgctaaccc
    97441 gcaggaaggt tctgttgttt ttgacgatca gatccgtggt ccgatcgagt tcagcaacca
    97501 ggaactggac gatcttatta tccgccgtac cgatggttcc ccaacctata acttctgtgt
    97561 ggttgtcgat gactgggata tggaaatcac ccacgttatc cgtggcgaag accatatcaa
    97621 caacacgcca cgccagatca acattcttaa ggccctgaaa gcgccggtgc cggtttacgc
    97681 gcacgtttct atgatcaacg gcgatgacgg taaaaaactg tccaaacgtc acggggcggt
    97741 cagcgtaatg cagtatcgtg atgacggtta tttgccagaa gcactgctga actatctggt
    97801 gcgtctgggc tggtcccacg gcgatcagga aatcttcact cgtgaagaga tgatcaaata
    97861 cttcaccctg aatgccgtca gcaaatctgc cagtgcgttc aacaccgaca agctgctgtg
    97921 gctgaaccat cactacatta acgcgctgcc gccggagtat gttgctactc acttacagtg
    97981 gcacattgag caggaaaata tcgatacccg taacggcccg cagctggctg atctggtgaa
    98041 actgctgggc gaacgctgca agacgctgaa agagatggca cagagctgcc gttatttcta
    98101 cgaagatttt gctgagttcg atgccgacgc cgcgaaaaaa catctgcgtc cggtagcgcg
    98161 tcagccgctg gaagtggttc gtgacaaact ggccgcgatt actgactgga ccgctgaaaa
    98221 cgttcatcac gctattcagg cgacggcgga tgagctggaa gtgggtatgg gtaaagttgg
    98281 tatgccgctg cgtgtcgccg taaccggtgc ggggcagtct ccggcactgg atgttaccgt
    98341 tcacgcaatt ggtaggaccc gcagtatcga gcgtatcaac aacgcgctgg cttttattgc
    98401 tgaacgcgaa aaccagcagt aattagcgca taaaagataa acggcaggag ataatatcct
    98461 gccgtttttt atttatgctg attcgtcaat atcgagacgg gcgaggaaat tgcgaattcc
    98521 ttcacgtgac aaaaattgcg cgactttttc ctgctcaatg tctgtcatat tatctatcgc
    98581 gttgatgatt tgtcgatacg ccggggcgcg tgctccagga gcgtattcag caattctctc
    98641 ttcagcttcc atcaacattg gtgtgttatg gaaggctggc gtgttctgaa taaactcgta
    98701 aacttgcgta tcgaccagaa taagtctggc acgaccgcct ttcacgccgg gaaatttttc
    98761 cgttttccag cctttttctc tgatccaacg gttaacagtt tgtttagcca caccgaggca
    98821 ttcagccagt tcttcggtgg tcattttatt gcgtaatctt ttcatattat ttgctatcgc
    98881 gaatccctaa gcgttgtaac aatccgataa tcccttcccg cagaagcaag gatgtaaact
    98941 gtttttgttc aactggcgtc atctctttcg ccagtgtcac cagcaatact tcaagtggcg
    99001 catcaccaga aagggcaggc tcttctccct gaccttctgg acgttcagca ttgcgaatat
    99061 attcacgaac ctgttcattg acgtgaacca gtcgggcttt gccaccctgg acgccaggtt
    99121 ttggtgacgt tgtccagcct tccttgcgta cccatttatt aatggtctgg cggctatagc
    99181 cggtcagtct ggcaagttca tctggtgtca tcctctcctt gaacataaca attccctgaa
    99241 tgtatgtggg tctattagta gttcatttta taacaccact ttacaggaaa ccgtgacatg
    99301 aataactact gaatgcgagc ggttttccaa aattgttcat ttgcctgatt tttcagcgat
    99361 tgaaactaca cgttgcaatt tgccgttgac acattcgggc ggaattcata tgatgccgcc
    99421 cgtcaactcg acaagcttta cgtgacgggg ctatagctca gctgggagag cgcttgcatg
    99481 gcatgcaaga ggtcagcggt tcgatcccgc ttagctccac caaatttcca accctcgctg
    99541 caaagcgggg gttttttgtc tctgcttttt gccgcttttg taatacagtc tacgtccggg
    99601 ttagtgccgc ctggtgaaag catcattgga tgaaaaatcg gcaacaggct ggccccctgt
    99661 ttgcttcgcg atgcgaataa acttattatt tgtgtgcctg aaaaccccga tcagtgagag
    99721 tagtgtactc atgtttgtgg agcataacct gataaaaaat atcaagatat tcacactagc
    99781 gtttacgctc accgtggtac ttattcagct atcccgtttt atttcgccac ttgccattat
    99841 ccattccagt tatatctttc tggcgtggat gccactgtgc gtaatgctgt caatcttgtt
    99901 tatctttggc tggcgcggtg tcgttcccgt tttatgcggg atgttttgca ccaatctgtg
    99961 gaactttcat ctctcttttt tacagactgc ggtcatgctc ggtagccaga cgtttgtcgt
   100021 gttgtgtgcc tgcgcaatat tacgctggca gctggggacg cgttggcgtt atggattgac
   100081 cagccgatat gtctggcaac gtctgttctg gcttggtttg gtgacgccga tcggcatcaa
   100141 atgcagcatg tatcttgtgg ggagcttctt tgatttcccg ctaaagatat ccaccttttt
   100201 cggcgatgcg gatgccattt tcacggtcgt tgatttgcta agccttttca ccgctgtgct
   100261 gatttacaac atgcttttct actatctcac tcgcatgatt gtaagtcccc actttgcgca
   100321 gatattgtgg cgcagggata tcgcgccgtc gttgggcaaa gagaaacgcg catttacctt
   100381 aagctggctg gcagcgttaa gcgtgctgct acttctgttg tgcacacctt atgaaaacga
   100441 ctttattgcc ggttacctgg tacccgtttt cttcatcatc tttaccctcg gggtcggtaa
   100501 gcttcgctat ccgtttttaa atctcacctg ggctgtttca acgctttgcc ttctgaatta
   100561 caaccagaac tttttgcaag gggtggaaac cgaatattcg ctggcattta ttcttgcggt
   100621 gctgatttcc tttagcgttt gcctgctcta tatggtgcgc atttatcatc gcagtgaatg
   100681 gcttaatcgc cgctggcatt tgcaggcgct gaccgatccg ttaacactcc tgcccaactt
   100741 ccgtgcgttg gaacaagcgc cggagcaaga ggcgggcaag agtttttgct gcctgcgcat
   100801 tgataatctt gagtttatga gtcgtcatta cggcttaatg atgcgcgttc actgtatccg
   100861 atcaatttac cgtacgctgc tgccgttgat gcaggaaaac gaaaagttat atcaattgcc
   100921 gggtagtgaa ctgctgttag tgctgagcgg gccggaaacg gaagggcgac tccagcatat
   100981 ggttaacatc ctgaatagtc ggcaaattca ctggaacaat accgggctgg atatgggcta
   101041 tggtgctgcc tgggggcgtt ttgatggaaa tcaggaaacc ctgcaaccct tgttggggca
   101101 gttaagctgg ctggcggagc aatcctgcgc acatcatcat gtgctggcgc tggatagcag
   101161 agaggagatg gtttccgggc agaccactaa acaggtgcta ttgctgaata ccattcgcac
   101221 ggcgttagat cagggtgatt tgctgctcta cgcccagcca attcgcaaca aagagggtga
   101281 aggttatgat gagatcctcg cgcgactgaa atatgacggc ggcattatga ccccggataa
   101341 gtttctgccc cttattgctc agtttaacct tagcgcgcgt tttgatttgc aagtgctgga
   101401 atccttgttg aagtggctgg caacacaccc ttgcgacaaa aaaggaccgc gcttttcagt
   101461 caatttaatg ccgctcacgc tgctgcaaaa gaatattgcc gggcggatta ttcgtctgtt
   101521 taagcgttat cacatctccc cgcaggcggt cattcttgag atcaccgagg agcaggcgtt
   101581 ttctaacgca gaaagcagca tgtacaacat cgagcagctg cataagtttg gtttccggat
   101641 tgcgattgat gactttggca ccggatatgc caactacgaa cggttaaagc gtttgcaggc
   101701 tgatatcatc aaaattgatg gcgtctttgt gaaagatatt gtcacgaaca cgctggatgc
   101761 gatgattgtg agatcaatta ccgatctggc gaaagcgaag tcattgagtg tggtcgcgga
   101821 gtttgtcgag acgcaacagc agcaggcgct attgcataag ctcggggtgc aatatctgca
   101881 agggtatttg attggtcgcc cgcagccatt agctgattaa caggcgtaaa aaaaccgggg
   101941 aattatcccc ggctttttta tgtatggtct tacagcacca gtgctgcgat tgacgcagac
   102001 agcacactca ccagggtaga gccgtaaacc agcttcagac cgaagcgaga aaccacgtta
   102061 ccttgctctt cattcaggcc tttaactgca cctgcgataa tcccgattga agagaagtta
   102121 gcgaaggaaa ccaggaacac agagatgatg ccttcagcac gcggagagag cgtggaagca
   102181 attttctgca gatccatcat cgcaacgaac tcgttggaaa ccagtttggt cgccatgata
   102241 ctgcccactt gcagtgcttc actggaagga acacccatca cccatgcaat cggatagaag
   102301 atgtagccca ggatgccctg gaaggagatg ctgtagccaa accagccagt aacggtggca
   102361 aacagtgcgt tcagcgcggc gatcagggcg ataaagccga tcagcatcgc ggcaacgata
   102421 atggcaactt tgaaacctgc cagaatgtat tcacccagca tttcgaagaa gctctgacct
   102481 tcgtgcaggt tggacatctg gatgttttct tcactggcat caacacggta aggattgatc
   102541 agcgacagca cgataaaggt gctgaacatg ttcagtacca gcgcagcaac gacgtatttc
   102601 ggttccagca tggtcatgta tgcaccaacg atggacatcg acacggtgga cattgccgtg
   102661 gcagccatgg tgtacatacg attacgggag attttgccga ggatatcttt ataggcaata
   102721 aagttttcag actgacccag aatcagggag ctgacggcgt taaaggattc cagtttgccc
   102781 atgccgttga ctttggagag caggaaacca attgcgcgga tgatcaccgg caacacgcga
   102841 atgtgctgga gaataccgat cagtgcagag ataaagacga ttgggcacag cactttcagg
   102901 aagaagaatg ccaggccttg atcattcatg ctaccaaaga cgaagttagt cccttcgttg
   102961 gcaaatccga gcagtttttc gaacatttcg gagaagcctt tcacgaagcc taaaccaacg
   103021 tcggagttca ggaagaacca cgccagtaac acttcgataa caagcagttg aataacataa
   103081 cggatacgaa tttttttgcg gtcgctgctt accagcagtg cgagaatcgc aacaacggca
   103141 agtgccagta caaaatgaag gacgcggtcc atatttgctc caaatatgag gcaggttaaa
   103201 tttccgtgca cattctatgt aacatggtta aggaaaacga gatctgacac acactataat
   103261 gacgtcctgt gacaagcttc aaaaatagtg atctgtcata catttctgct atgttgtgta
   103321 tggaaggtga aagttatgta aatgtgctaa cattaaacat aaccataaca ttcaagtcat
   103381 caatcttttc gtagtcataa tcctggtcta tcaaagaaat caccacaacc cattcaaatg
   103441 aatttgataa tcattctcgt ttggcatagc atgaaacata gcaaaggcta tgttttagag
   103501 gcacaagatg acgaactatc gcgttgagag tagcagcgga cgggcggcgc gcaagatgag
   103561 gctcgcatta atgggacctg cgttcattgc ggcgattggt tatatcgatc ccggtaactt
   103621 tgcgaccaat attcaggcgg gtgctagctt cggctatcag ctactgtggg ttgtcgtttg
   103681 ggccaacctg atggcgatgc tgattcagat cctctctgcc aaactaggga ttgccaccgg
   103741 taaaaatctg gcggagcaga ttcgtgatca ctatccgcgt cccgtagtgt ggttctattg
   103801 ggttcaggca gaaattattg cgatggcaac cgacctggcg gaatttattg gtgcggcgat
   103861 cggttttaaa ctcattcttg gtgtttcgtt gttgcagggc gcggtgctga cggggatcgc
   103921 gactttcctg attttaatgc tgcaacgtcg cgggcaaaaa ccgctggaga aagtgattgg
   103981 cgggttactg ttgtttgttg ccgcggctta cattgtcgag ttgattttct cccagcctaa
   104041 cctggcgcag ctgggtaaag gaatggtgat cccgagttta cctacttcgg aggcggtctt
   104101 cctggcagca ggcgtgttag gggcgacgat tatgccgcat gtgatttatt tgcactcttc
   104161 gctcactcag catttacatg gcggttcgcg tcaacaacgt tattccgcca ccaaatggga
   104221 tgtggctatc gccatgacga ttgccggttt tgtcaatctg gcgatgatgg ctacagctgc
   104281 ggcggcgttc cacttttctg gtcatactgg tgttgccgat cttgatgagg cttatctgac
   104341 gctgcaaccg ttgttaagcc atgctgcggc aacggtcttt ggattaagcc tggttgctgc
   104401 cggactgtcc tcaacggtgg tggggacact ggcagggcag gtggtgatgc aggggttcat
   104461 tcgcttccat atcccgctgt gggtgcgtcg tacagtcacc atgctgcctt catttattgt
   104521 cattctgatg ggattagatc cgacacggat tctggttatg agtcaggtgc tgttaagttt
   104581 tggtatcgcc ctggcgctgg ttccactgct gattttcacc agtgacagca agttgatggg
   104641 cgatctggtg aacagcaaac gcgtaaaaca gacaggctgg gtgattgtag tgctggtcgt
   104701 ggcgctgaat atctggttgt tggtggggac ggcgctggga ttgtagttga atgagcgtcg
   104761 catctggcac tattggcgga tgcggcgtaa acgccttatc cgccctacgc gttaaagagc
   104821 cggtttgtag gcctaataag acgcgctagc gtcgcatcag gcattgtgct ccattggagg
   104881 acgcgtttaa tggcggtgcc catgtccgcg gccttttccg cgatgatcgt cacgatcgcg
   104941 ccagccttca cgatagccac gctcatatgc tttacgctta tcccagccac gatgatagcc
   105001 attatcatga cgccaccagc ggtttttgcg ccattcataa ttacgatgcc agtaatcacg
   105061 atctcgccag tgaccaccgt cccagtaatt accgtaatga tcgcgatcgc caatttgtaa
   105121 tttgatcgat ggcagtaggg tgatttcacc tgcatttgct gcaagcgggg taaatgccat
   105181 cagggcggcc gccagaaaca gtgacctgaa cattgttatt ctccttcacg atcgaagccg
   105241 tcagcggcct gttaacgcaa tattacgggg aggtaaagcc ccgtatcatc gccataactc
   105301 ttaaatcatt aaggagagca ttttttgcta aacattatcg tggggtggct gttgcttcgg
   105361 acgattgacc atcatcatcg gcttacctgc taataacagc catgccggaa gcatgacgat
   105421 acagagcagc ggtagcaatg tggtattggg tacaacgact gccgccataa aaagacttaa
   105481 ccagccatcg cgtgttacca ccagcacgat gccgagaata gcgcaggaaa cggttattgc
   105541 cgctggaacg gcgggaacgt gctcatgcag catcaaccct aatgccacgc cgacaaacac
   105601 tgccgggaag attcgcccac cgcgaaagcc actagcggca gcaacgacca gggcggcaag
   105661 tttaattacc gccagcaaaa agtaatcgct ggtgctgaaa gcctgatttg ccaccatctg
   105721 ctgcatctca tccagccctt taaacagcga aactggtcca ccaataaccc ccagaattcc
   105781 gagaataaat ccgccaatac ccagcacgag caccggattt ttcatttgat tcatcatcgc
   105841 gtgcaaccgt ggtaagcacc atacggcgac catccctgct gcgatggcaa tcgccgcgac
   105901 aattgcacca ctgagaatat cggtcatttc catctgtccg taatgcgcaa tgggcagtga
   105961 aaaatgggga tggaaaaata atccggtagt aagtgcacca gctgctgccg ccattaacgg
   106021 cgcaaaaaga cgatcccata gcggaacttc actactgcca tttaaggttt gcgaaaatat
   106081 caacgccgcc gcaacaggtg tgccaaacag cgcgccgatg gttccggcag aggctaaaat
   106141 agtccactcc attcggttga cgcgcggtaa cagacgagcg ccaatggcaa ccgcaagggc
   106201 gatattgacg gtcatgatcg gatgttccgg ccccaggctg acgccgccag caagaccgag
   106261 aattaatgct acgataagtc ctggtagcgc ggaaggtgga accggtgcgc cgatcagagg
   106321 ttcacaggcg gggtctggtc cggcatgacc ctggctgaag cggataacca accccaccgc
   106381 aatacctgtt agcgttaata caccgatgat ccagaaaggt gaatcctggg ctatccccag
   106441 agttcccggc agtcgttgcc agagcaaatt ctgtaatgcc gaggcgattt tcatcaccac
   106501 aatcagaata agactggacg caatcccaat tgccacggcg gggagcgata ataacaacat
   106561 ggttctggct cgcggatgga gcatgttcta ttccttatgc ggggtcagat acttagtttg
   106621 cccagcttgc aaaaaggcat cgctgcaatt ggtgctgaaa cgataaagta attgtgtgac
   106681 ccagatcgat atttacaggg agcctgcctt tccggcgttg ttgttatgcc cccaggtatt
   106741 tacagtgtga gaaagaatta ttttgacttt agcggagcag ttgaagaatg acaaagtatg
   106801 cattagtcgg tgatgtgggc ggcaccaacg cacgtcttgc tctgtgtgat attgccagtg
   106861 gtgaaatctc gcaggctaag acctattcag ggcttgatta ccccagcctc gaagcggtca
   106921 ttcgcgttta tcttgaagaa cataaggtcg aggtgaaaga cggctgtatt gccatcgctt
   106981 gcccaattac cggtgactgg gtggcaatga ccaaccatac ctgggcgttc tcaattgccg
   107041 aaatgaaaaa gaatctcggt tttagccatc tggaaattat taacgatttt accgctgtat
   107101 cgatggcgat cccgatgctg aaaaaagaac atctgattca gtttggtggc gcagaaccgg
   107161 tagaaggtaa gcctattgcg gtttacggtg ccggaacggg gcttggggtt gcgcatctgg
   107221 tccatgtcga taagcgttgg gtaagcttgc cgggcgaagg cggtcacgtt gattttgcgc
   107281 cgaatagtga agaagaggcc attatcctcg aaatactgcg tgcggaaatt ggccatgttt
   107341 cggcggaacg cgtgctttct ggccctgggc tggtgaattt gtatcgcgca attgtgaaag
   107401 ctgacaaccg cctgccagaa aatctcaagc caaaagatat taccgaacgt gcgctggctg
   107461 acagctgcac cgattgccgc cgcgcgttgt cgctgttttg cgtcattatg ggccgttttg
   107521 gcggcaatct ggcgctcaat ctcgggacat ttggcggcgt gtttattgcg ggcggtatcg
   107581 tgccgcgctt ccttgagttc ttcaaagcct ctggtttccg tgccgcattt gaagataaag
   107641 ggcgctttaa agaatatgtc catgatattc cggtgtatct catcgtccat gacaatccgg
   107701 gtcttctcgg ttccggtgca catttacgcc agaccttagg tcacattctg taaatccttc
   107761 ctgttatatc gggaggtaac tctcccgata atcttttaaa tcatacagtt aattcaattt
   107821 ttctttgtgt cctcctcact aggccgacct gcgtcacact tccgtacagc gggattaatt
   107881 ctccagtaaa tgcattattt gtctggtaac ggcgatttgt tttgcacgtt cataatttca
   107941 ctcgtcaact atcactgaac gaggaattaa cgatgagtaa gaaactgatt gccttatgtg
   108001 cctgcccaat gggcctggct cacactttta tggccgctca ggcgctggaa gaagcggcgg
   108061 tagaagccgg ttatgaagtg aaaattgaaa cccagggcgc ggacggtatc cagaatcgcc
   108121 tgacggcgca ggatatcgcc gaagcaacca tcatcatcca ctccgtggca gttaccccgg
   108181 aagataatga gcgtttcgaa tcacgtgacg tttatgaaat cactttgcag gacgcaatta
   108241 aaaacgctgc gggcatcatc aaagaaatcg aagagatgat tgcctctgaa caacagtaat
   108301 attcgacagg gataattgtt atggccatta aaaaacgcag tgcaaccgtt gtgcctggcg
   108361 catccggtgc ggcagcggca gttaagaatc cgcaggcctc taaaagcagc ttctggggtg
   108421 aactcccgca gcatgtgatg tcagggattt cacgcatggt gccaacctta attatgggcg
   108481 gtgtgatcct cgctttcagc cagctgattg cttatagctg gcttaaaatt cctgctgaga
   108541 tcggcatcat ggatgctctt aatagcggga aattctccgg tttcgacctc tccttactta
   108601 aatttgcctg gctgtcgcag tccttcggcg gcgtgttgtt tggttttgcc attccgatgt
   108661 ttgctgcttt tgtggcgaac tctatcggtg gcaaactggt gttcccggct ggttttatcg
   108721 gcgggttgat gtctacccag ccgacgcaac tgctgaactt cgatcccagc actatgcaat
   108781 gggcgacctc ttcgccggtg ccgtcaacct tcattggtgc gctgattatt tctatcgtcg
   108841 cgggttacct tgtgaagtgg atgaaccaga aaatccagtt accggatttc ctgttagcgt
   108901 tcaaaaccac atttttgcta ccgattcttt ccgccatttt tgtcatgctg gcgatgtact
   108961 acgtcatcac cccctttggt ggctggatca acggcggtat ccgtactgtg ctgactgccg
   109021 caggtgagaa gggcgcgctg atgtacgcaa tggggatcgc cgcggcgaca gcaatcgacc
   109081 ttggtggccc gatcaacaaa gccgcaggtt tcgttgcctt cagctttacc actgaccacg
   109141 tattaccggt caccgctcgt tctatcgcta tcgttattcc tccgattggt ctgggtctgg
   109201 cgaccattat tgaccgtcgt ttaaccggca aacgcctgtt caacgctcaa ctttatccgc
   109261 agggtaagac cgcgatgttc cttgccttta tggggattag tgagggtgcg attccgtttg
   109321 cgctggaaag ccccatcacc gccattccgt cgtatatggt cggcgcgatt gtcggctcaa
   109381 ccgccgctgt ctggctgggc gcagtgcaat ggttcccgga atccgctatc tgggcatggc
   109441 cgctggtcac taacctgggc gtctatatgg cggggatcgc gctgggggca gtcataactg
   109501 cgctgatggt ggtgttcctg cgtctgatga tgttccgtaa aggcaaattg ttaatcgata
   109561 gcctgtaaga aggaaaatcc agatgacatt actcgcttcg ctgcgcgact ggcttaaggc
   109621 gcaacaactg gatgcagtgc ttctctcctc acggcagaac aaacagccgc atctggggat
   109681 ctccaccgga tcaggctatg tggttattag ccgtgaaagt gcgcacattc tggtagattc
   109741 gcgctatttc gttgaggtgg aagcccgtgc gcaaggctac cagctgcatt tgcttgacgc
   109801 gacgaacacg cttaccacta tcgtcaatca aatcattgcc gatgagcagt tgcaaacgct
   109861 cggttttgaa ggccagcagg tgagttggga aaccgcgcac cgctggaagt ctgaacttaa
   109921 tgcgaaactg gttagcgcca cgccggatgt gctgcggcaa atcaaaacgc cagaggaggt
   109981 ggagataatc cgcctcgcct gtgggattgc cgatcgcgga gcggagcata ttcgccgctt
   110041 tattcaggcg gggatgagcg agcgcgagat agccgctgaa ctggagtggt ttatgcgcca
   110101 gcagggcgca gaaaaagcct cttttgacac cattgtcgcc agcggctggc gcggggcgct
   110161 gccgcacggc aaagccagcg acaagattgt tgcagcgggc gagtttgtca ctctcgattt
   110221 tggtgcgctg tatcagggct actgctctga tatgacgcgc accttgctgg tgaatggcga
   110281 aggggtgagc gccgaatctc acccgctgtt tgacgtctat cagattgtcc tgcaggcaca
   110341 gctcgcggca atctctgcaa ttcgccccgg cgtgcgctgc cagcagattg acgacgccgc
   110401 gcgccgggtc attaccgagg ctggatttgg cgactatttc ggtcataaca ctggtcacgc
   110461 tatcggcatt gaagtccatg aagatccgcg tttttcaccg cgggacacca cgacgctaca
   110521 gccaggcatg ttgctgaccg tggagccggg gatttatttg ccagggcaag ggggcgtgcg
   110581 catcgaagat gttgtgctgg ttaccccgca aggcgcagaa gtgctctacg ccatgccgaa
   110641 aacagtgttg ctcacgggag aggcataatg gatttatcgc tattaaaagc gttgagcgag
   110701 gcagatgcga tcgcctcctc ggaacaggaa gtgcggcaga tcctgctgga agaagcggat
   110761 cgcctgcaaa aagaagtgcg atttgatggt ctgggatcgg tgctgatccg cctcaatgaa
   110821 tcgacaggtc cgaaggtgat gatctgcgcc catatggacg aagtgggatt tatggtgcgc
   110881 agcatctccc gcgaaggcgc tattgacgtg ctgccggttg gcaacgtgcg catggctgcc
   110941 cgccagctgc agccggtgcg catcaccacc cgtgaagagt gcaaaattcc aggcctgctt
   111001 gacggcgact ggcaggggaa tgacgttagc gccatgcgcg tggacattgg cgcgcgcacc
   111061 tatgacgaag tgatgcaggc gggaattcgt ccaggcgatc gcgtcacctt cgataccact
   111121 tttcaggtcc tccctcacca gcgggtgatg ggcaaagcct ttgatgatcg cctcggttgc
   111181 tacctgctgg tgacgttact gcgcgaacta cacagcgctg aactgcctgc ggaagtgtgg
   111241 ctggtggcca gttccagcga agaggtgggg ttacgcggcg ggcaaactgc cacccgcgcg
   111301 gtgtcgccgg atgtcgccat tgtgctttat accgcctgct gggcgaaaaa ctttgattat
   111361 ggcgcggcta accatcgtca gattggtaac gggccgatgc tggtgttaag cgataaatcg
   111421 ctgattgcgc cgccaaaact taccgcgtgg attgaaaccg tgtcggcaga aattggcgtt
   111481 ccgttgcagg cggatatgtt cagtaacggc ggcacggacg gtggagcggt gcacttaacc
   111541 ggtactggcg tacccacagt ggtaatgggg cctgccaccc gccacggaca ttgcgcggca
   111601 tcgattgccg attgccgcga cattttgcag atggagcaac ttttatctgc ccttattcaa
   111661 cgtcttacgc gtgagacggt tgttcaactg acggatttca gatgatctcc tgattaaccc
   111721 ggagcggtta tgttaacgat tcaatttctc tgtcctttgc ctaacggtct acatgctcgt
   111781 ccggcgtggg aacttaaaga acagtgcagc cagtggcaaa gcgaaatcac ctttatcaac
   111841 catcgccaga acgcaaaggc cgatgctaaa agctcgctgg cgttgattgg aactggcacc
   111901 ttatttaatg acagttgcag cctgaacatt agcggcagcg atgaagagca ggcgcggcgc
   111961 gtgctggaag agtacatcct ggtgcgcttt atcgacagcg acagcgttca gccgacgcag
   112021 gcagaactga cggcgcatcc gctgccgcgt tcattaagcc gccttaaccc ggatttactg
   112081 tacggcaatg tgctggcaag cggcgtcggc gtgggtacgc tgaccctgtt acaaagcgac
   112141 agcctcgaca gttatcgggc aatccccgcc agtgcgcaag attccacccg gctggagcac
   112201 agccttgcaa cgcttgccga gcaactgaac cagcaattgc gtgagcgtga cggcgaaagc
   112261 aaaactatcc tcagcgccca tttgtcgctg attcaggatg atgaattcgc gggcaatatc
   112321 cgtcgtctga tgacagaaca gcatcaaggg ctgggggcgg cgatcatcag caatatggag
   112381 caggtttgcg ccaaactttc tgcttctgcc agcgattatc tgcgcgagcg ggtcagcgac
   112441 attcgcgata tcagcgaaca gttgctgcat atcacctggc cggaactgaa gccgcgcaac
   112501 aatctggtgc tggaaaaacc gaccattctg gtggctgaag atttaacccc aagccagttt
   112561 ttgagccttg atttgaaaaa tcttgcgggc atgattctgg agaaaaccgg gcgcacctcg
   112621 catacactga ttctggcccg tgcctcggcg atcccggtac tgagtggctt gccgctggat
   112681 gcgattgccc gttatgccgg gcaacctgca gtgcttgacg cccagtgcgg cgtgctggcg
   112741 attaacccga atgacgcggt gagcggttat tatcaggtcg cgcagacgct ggcggataaa
   112801 cgccaaaaac aacaggcgca ggctgccgcg cagctggcct attcccgtga taacaagcgt
   112861 attgatattg cggcgaatat cggcaccgct ctggaagcgc caggcgcgtt tgccaacggc
   112921 gcggaaggtg tcgggctgtt ccgtaccgaa atgctctata tggatcgcga cagcgcgccg
   112981 gacgagcagg agcaatttga agcctaccag caggtgctac tggcggcggg cgacaagccg
   113041 attatcttcc gcacgatgga catcggcggc gataaaagca ttccttatct gaatattccc
   113101 caggaagaga acccgttcct cggctatcgc gcggtacgta tttacccgga atttgctggc
   113161 ctgttccgca ctcaactgcg ggccattttg cgcgccgcca gtttcggcaa cgcccagttg
   113221 atgatcccga tggttcacag cctcgatcag atcttatggg tgaaaggcga gatccaaaaa
   113281 gcgatcgttg agcttaagcg cgatggcctg cgtcatgcag agacgattac gcttgggatc
   113341 atggtggaag ttccgtcggt gtgctacatc atcgaccact tctgcgatga ggtcgatttc
   113401 ttcagtatcg gctccaacga tatgacccag tatctgtatg cggtcgatcg taataacccg
   113461 cgcgtatcgc cgctatataa cccgattacg ccatcgttcc tgcgcatgtt gcagcaaata
   113521 gttaccactg cgcatcagcg gggcaaatgg gtaggcattt gcggtgaact gggcggtgaa
   113581 agccgttatc tgccgctact gcttgggctg ggcctggatg agctgagtat gagtagcccg
   113641 cgtattccgg cggtgaaaag ccagcttcgt caactggata gcgaggcgtg tcgggaactg
   113701 gcgcgtcagg catgtgaatg ccgcagtgca caggaaattg aagcgttact caccgccttt
   113761 acgccggaag aagacgttcg cccactgctg gcgctggaga atatctttgt tgatcaggat
   113821 tttagcaata aagagcaggc gatccagttc ctgtgcggca acctcggcgt taacgggcgc
   113881 actgaacatc cgtttgagct ggaagaagat gtctggcagc gggaagagat tgttaccacc
   113941 ggcgttggtt ttggcgtagc gatcccgcac accaaatctc agtggatccg tcattccagt
   114001 atcagcattg cccggctggc gaaaccggtt gactggcagt cagaaatggg cgaagtcgaa
   114061 ctggtgatca tgctgacgct gggtgctaac gaagggatga atcatgtgaa agtcttctcg
   114121 cagctggcgc gtaaactggt gaataaaaac ttccgccagt cgttgtttgc cgcgcaagat
   114181 gcacaaagta tcctgacgct gctggaaaca gaattaacct tctgacgtta gccctgaaaa
   114241 cgggcgctgt actctcccgg cgtcagacca aactgacgcc ggaaaacgcg acaaaaatag
   114301 tcgctatccg gaaaaccgca acgctgtgcc acttcatgaa ttgacagatg atatttctgc
   114361 aaaatcatcc tcgccttcgc cattcgcacc caacgcacat actcgataaa acgcatcgtt
   114421 ccatgctgag caaacagttt tgacagatga ttgggcgtga tattaaaaaa ctgcgccacg
   114481 ctctcgcggg tgagcggctg ggcataatta tcctgaaccc agttacaaat gctgtgatag
   114541 agaaaatcgc cgcgcggttg cgaattaccc ggcggcatat tcaccacgtt gcggcaaaga
   114601 tgcagcaaac ttaacaccag tggctgaatg atattctgct ccagcggcga acggctaaga
   114661 tgagtgaggg cggtaagcat cgcttcacct tcaccgcgtt gcaggctggg aagctggatc
   114721 tggcgagcag gatgcaacaa cgacgtggtg cggctatcgt aaaacgataa cccaagccag
   114781 gtcggtgcga acaccaggct taacagcatc acctgtttgt tgttgatcgg taaattagcc
   114841 gcgcgtgccg gaataaacag catttctcct tccaccattc gctgctgctt tgcttccagc
   114901 aggttgccgt attcgccgcg cagcacgata tccagacggg gaaaatcaat acataaactg
   114961 cccaccggta acgaggcagg ctggctggca aaccagacgc gccccagtag ttgcgggtta
   115021 agcaccaggc cgctgaacag atcggcgaaa aattgttgat cggcgggcaa gcctggtgct
   115081 ttcatgaaag ttcccgatta aagatgcatt aactggcgaa attctttcac tttgctgcgg
   115141 ctgaccggca cttcaaaatc cagatctttc agtcgcagaa tgtaggtgtt attaaaccac
   115201 ggttcgattt cgcgtatttt gttcagattg acacaaaatg agcgatggca gcggaaaaaa
   115261 tgcgacggcg gcagtttgct gcaaaattcg gtaatgttca tcggcattac gtaggattca
   115321 cgccgcgtat agacaaaggt cattttctcg tgcgcttcgg cgtaatagat atcgttaatt
   115381 ggcgtgacga ttattcgctc atctttcacc agattaatgg tgtcattctc acgcgttacc
   115441 gtcgcggcag gcgtactgct ggtctgctgt tgttgccagg ccgcttccag tttttgcagc
   115501 atcccggtaa tacgtgactc ctgatacggt ttgagaatgt agtcaaacgc ctccagttca
   115561 aacgcttcta ccgcatgttc tttccacgcg gtgatgaaca caataaacgg tttatgggcg
   115621 aactggctga tgttttgcgc cagcaacacg ccatccagcg acggaatatt gatatccaga
   115681 aaaatggcgt cgacgcggtt atgctgcaaa aacttcaaca cgtccagacc gtcgtcaaag
   115741 gtgccgacaa tctccatctg gctgtgctct ttaattagcc agctcagttc ctgttgtgcc
   115801 aggaattcgt cttcaacaat gatgactttc acaatatcac tccggctcaa agcaataacg
   115861 tagcctgtga ggcgactggg gtgcgttggt taggaatgta aaacgcaatt tccgtccccg
   115921 gctccaggcg gcggatatgc agcccctcgc catacaataa cttcacgcga tgatggacat
   115981 tcagcaggcc aattttattg tccggcattt cattcgcttc gacccgctca atcacctttg
   116041 gatcgatgcc gtgcccggta tctcgcaccg caatgcgtac ccgatttccg cactctgcaa
   116101 cgctgatggt gacaacgcct ttacctttgc aaggctgaat accgtggaca atggcgttct
   116161 ccaccaacgg ctggatcagc aggctgggaa tgcagcaatt cacctcttca tcaatatcat
   116221 agataaccgt cagcttgtca ccaaagcggg cctgctcaat ggcaatatag tctttaattt
   116281 gatacagctc ttttttgata tcgatttgct catcgtcttt taattcaatg ttatagcgca
   116341 gataacgcga cagattaaag atcaactggc gagcggtatc cggattcaga cggattgacg
   116401 atgaaatagc gttcagagcg ttaaacagaa aatagggatt aattttgctt tgcagggcgc
   116461 gaagctctgc cttatttgcc atttcacgta gctgctcggc gcgtgaaacc tccagttgcg
   116521 tggagatgat ttgcgacaga ccaaccgcca tttcctgtaa tgacgaggtt atctgatgcg
   116581 cgtggcagta gtaaattttc agcgttccgg tcacaacgcc tttctcccac aatgggatca
   116641 ccagcatgga atgaatctct ggtgtgcggt gggcttcatc attgttttta atgatgattt
   116701 ttccgtaatt catcgcctga cgggtagtcg ggctaatgaa gtcatcgcca ttctgatagt
   116761 tatgttcacc catgccaaca taggccagca catgatcggt attagtaatc gccaccgcat
   116821 cggcgtgaat gtcatcgcgg ataatttcgc agaccttgcg taatgactcg ctattgacat
   116881 ggcgaaacag cggtagcgtt ttgttggcga tatccagcgc cagcttggcc tgccgcgcgg
   116941 cgctggcctc tttttcgccc tcaacgcttt gcaccagaag cacaataaag ccgatacaga
   117001 cgctaccgag gatcattgga atgccgattt tagagacgat atcgatcccc aacgcggtag
   117061 ttggtgccca gacaattacc agaatcatgg tcaacgtctc acacaacatg ccgccgagaa
   117121 taccgacgcg ccagcgctgt gctttgggga ttttcaggtt tatccagcct gatatacaac
   117181 ccgccagaat gctggtgata aagcagggga tcgccgtcac gccgccgata tcaattaaat
   117241 accggtgaat acccgcaatc acgccggtaa tgatgcctac ccacgggcca aacagaatcc
   117301 cgccggacat caccgcgata atacgtacgt tcaccagcga gccttctacg ggaacgccgg
   117361 accaggtgct gaacagggcg aacagcgaga aaatggcggt aacagcaagc aactcttttg
   117421 gggagtgtgc cgacttgtgc aacagctcgc gaaacaggcg gatacggatg agaaagaaca
   117481 ggcagataag cattaacgct gcccgatcga agaccgccag cagcatgttg aatatttcgt
   117541 gcacgggtga ctctgaataa ggattaaacc tctatgatag gtaacctgaa ggctgatgac
   117601 cagcaggccg tttttgagga aaaaccataa atttaaaagg ggattgaaag ataaatggtg
   117661 tattagcaat gtatctcttg ataatgcact tctggctggt aaatttattg aaattataat
   117721 gttaataaaa ttggtggtaa ttttcttgtt agcgtagaaa aa
//
LOCUS       DAZKHC020000003       100819 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZKHC020000003 DAZKHC020000000
VERSION     DAZKHC020000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 100819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 100819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.003"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            24..3758
                     /gene="yfaL"
                     /locus_tag="ACHOSM_RS01175"
                     /old_locus_tag="pgaptmp_000235"
     CDS             24..3758
                     /gene="yfaL"
                     /locus_tag="ACHOSM_RS01175"
                     /old_locus_tag="pgaptmp_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HGU4556834.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDSAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGRTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3886..4608)
                     /gene="ubiG"
                     /locus_tag="ACHOSM_RS01180"
                     /old_locus_tag="pgaptmp_000236"
     CDS             complement(3886..4608)
                     /gene="ubiG"
                     /locus_tag="ACHOSM_RS01180"
                     /old_locus_tag="pgaptmp_000236"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HGU4556835.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            4755..7382
                     /gene="gyrA"
                     /locus_tag="ACHOSM_RS01185"
                     /old_locus_tag="pgaptmp_000237"
     CDS             4755..7382
                     /gene="gyrA"
                     /locus_tag="ACHOSM_RS01185"
                     /old_locus_tag="pgaptmp_000237"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HGU4556836.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7531..9219
                     /gene="yfaA"
                     /locus_tag="ACHOSM_RS01190"
                     /old_locus_tag="pgaptmp_000238"
     CDS             7531..9219
                     /gene="yfaA"
                     /locus_tag="ACHOSM_RS01190"
                     /old_locus_tag="pgaptmp_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HGU4556837.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9216..9839
                     /gene="yfaT"
                     /locus_tag="ACHOSM_RS01195"
                     /old_locus_tag="pgaptmp_000239"
     CDS             9216..9839
                     /gene="yfaT"
                     /locus_tag="ACHOSM_RS01195"
                     /old_locus_tag="pgaptmp_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HGU4556838.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9863..14278
                     /locus_tag="ACHOSM_RS01200"
                     /old_locus_tag="pgaptmp_000240"
     CDS             9863..14278
                     /locus_tag="ACHOSM_RS01200"
                     /old_locus_tag="pgaptmp_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HGU4556839.1"
                     /translation="MGAGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYTTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGELPSGGKSFTVNFAKPGNY
                     NLTLRDKDGLILAGLSYAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFPEPIDE
                     ALLTLERDRVEQQSLLSHPANWLTLQRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQ
                     YSFQNAGIKVAVPQLDIRVKTDKTHYQLGELVNVELTSSLKGKPVSAQLTVGVVDEMI
                     YALQPEIAPNIGKFFYPLGRNNVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKML
                     ERPRREEVDTAAWMPSLTTDKQGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYL
                     RSEKNLYMKWSMPTVYRVGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGA
                     NYISLTQNIQQSGLLSAELQQNGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALM
                     LPEQASNIRLQSSETPQEIFRNNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSA
                     AANDIRQMIQDNRLRLMQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQ
                     QPEYWQHMLDSYAEQADNMPLLHRALVLAWAQEMNLPCKTLLKGLDEAIARRGTKDED
                     FSEEDIRDINDSLILDTPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAAN
                     SNQPLAHTVVLLNSGGDATQTAAILSGLTAEQSTIERALAMNWLAKYMATMPPVVLPA
                     PAGAWAKHKLTGGGEDWRWVGQGVPDILSFGDELSPQNVQVRWREPAKTAQQSNIPVT
                     VERQLYRLIPGEEEMSFTLQPVTSNEIDSDALYLDEITLTSEQDAVLRYGQVEVPLPP
                     GADVERTTWGILVNKPNAAKQQGQLLEKARNEMGELAYMVPVKELTGTVTFRHLLRFS
                     QKGQFVLPPARYVRSYAPAQQSVAAGSEWTGMQVK"
     gene            14279..15928
                     /gene="yfaQ"
                     /locus_tag="ACHOSM_RS01205"
                     /old_locus_tag="pgaptmp_000241"
     CDS             14279..15928
                     /gene="yfaQ"
                     /locus_tag="ACHOSM_RS01205"
                     /old_locus_tag="pgaptmp_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HGU4556840.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPEISVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            15933..16709
                     /gene="yfaP"
                     /locus_tag="ACHOSM_RS01210"
                     /old_locus_tag="pgaptmp_000242"
     CDS             15933..16709
                     /gene="yfaP"
                     /locus_tag="ACHOSM_RS01210"
                     /old_locus_tag="pgaptmp_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HGU4556841.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKIQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            16984..19833
                     /gene="rcsC"
                     /locus_tag="ACHOSM_RS01215"
                     /old_locus_tag="pgaptmp_000243"
     CDS             16984..19833
                     /gene="rcsC"
                     /locus_tag="ACHOSM_RS01215"
                     /old_locus_tag="pgaptmp_000243"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HGU4556842.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPGDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(19919..20569)
                     /gene="rcsB"
                     /locus_tag="ACHOSM_RS01220"
                     /old_locus_tag="pgaptmp_000244"
     CDS             complement(19919..20569)
                     /gene="rcsB"
                     /locus_tag="ACHOSM_RS01220"
                     /old_locus_tag="pgaptmp_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HGU4556843.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20586..23258)
                     /gene="rcsD"
                     /locus_tag="ACHOSM_RS01225"
                     /old_locus_tag="pgaptmp_000245"
     CDS             complement(20586..23258)
                     /gene="rcsD"
                     /locus_tag="ACHOSM_RS01225"
                     /old_locus_tag="pgaptmp_000245"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HGU4556844.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVYDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASCLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            23996..25102
                     /gene="ompC"
                     /locus_tag="ACHOSM_RS01230"
                     /old_locus_tag="pgaptmp_000246"
     CDS             23996..25102
                     /gene="ompC"
                     /locus_tag="ACHOSM_RS01230"
                     /old_locus_tag="pgaptmp_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU4556845.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSISGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            25214..26269
                     /gene="apbE"
                     /locus_tag="ACHOSM_RS01235"
                     /old_locus_tag="pgaptmp_000247"
     CDS             25214..26269
                     /gene="apbE"
                     /locus_tag="ACHOSM_RS01235"
                     /old_locus_tag="pgaptmp_000247"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HGU4556846.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            26343..27407
                     /gene="ada"
                     /locus_tag="ACHOSM_RS01240"
                     /old_locus_tag="pgaptmp_000248"
     CDS             26343..27407
                     /gene="ada"
                     /locus_tag="ACHOSM_RS01240"
                     /old_locus_tag="pgaptmp_000248"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HGU4556847.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            27407..28057
                     /gene="alkB"
                     /locus_tag="ACHOSM_RS01245"
                     /old_locus_tag="pgaptmp_000249"
     CDS             27407..28057
                     /gene="alkB"
                     /locus_tag="ACHOSM_RS01245"
                     /old_locus_tag="pgaptmp_000249"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HGU4556848.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVIWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            28133..29776
                     /gene="yojI"
                     /locus_tag="ACHOSM_RS01250"
                     /old_locus_tag="pgaptmp_000250"
     CDS             28133..29776
                     /gene="yojI"
                     /locus_tag="ACHOSM_RS01250"
                     /old_locus_tag="pgaptmp_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HGU4556849.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTYVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            29994..31640
                     /gene="mqo"
                     /locus_tag="ACHOSM_RS01255"
                     /old_locus_tag="pgaptmp_000251"
     CDS             29994..31640
                     /gene="mqo"
                     /locus_tag="ACHOSM_RS01255"
                     /old_locus_tag="pgaptmp_000251"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HGU4556850.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(31790..32299)
                     /gene="eco"
                     /locus_tag="ACHOSM_RS01260"
                     /old_locus_tag="pgaptmp_000252"
     CDS             complement(31790..32299)
                     /gene="eco"
                     /locus_tag="ACHOSM_RS01260"
                     /old_locus_tag="pgaptmp_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HGU4556851.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(32413..32577)
                     /locus_tag="ACHOSM_RS01265"
                     /old_locus_tag="pgaptmp_000253"
     CDS             complement(32413..32577)
                     /locus_tag="ACHOSM_RS01265"
                     /old_locus_tag="pgaptmp_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4556852.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCPLIRI"
     gene            32686..33180
                     /gene="napF"
                     /locus_tag="ACHOSM_RS01270"
                     /old_locus_tag="pgaptmp_000254"
     CDS             32686..33180
                     /gene="napF"
                     /locus_tag="ACHOSM_RS01270"
                     /old_locus_tag="pgaptmp_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HGU4556853.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            33170..33433
                     /gene="napD"
                     /locus_tag="ACHOSM_RS01275"
                     /old_locus_tag="pgaptmp_000255"
     CDS             33170..33433
                     /gene="napD"
                     /locus_tag="ACHOSM_RS01275"
                     /old_locus_tag="pgaptmp_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HGU4556854.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            33430..35916
                     /gene="napA"
                     /locus_tag="ACHOSM_RS01280"
                     /old_locus_tag="pgaptmp_000256"
     CDS             33430..35916
                     /gene="napA"
                     /locus_tag="ACHOSM_RS01280"
                     /old_locus_tag="pgaptmp_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HGU4556855.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            35923..36618
                     /gene="napG"
                     /locus_tag="ACHOSM_RS01285"
                     /old_locus_tag="pgaptmp_000257"
     CDS             35923..36618
                     /gene="napG"
                     /locus_tag="ACHOSM_RS01285"
                     /old_locus_tag="pgaptmp_000257"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HGU4556856.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            36605..37468
                     /gene="napH"
                     /locus_tag="ACHOSM_RS01290"
                     /old_locus_tag="pgaptmp_000258"
     CDS             36605..37468
                     /gene="napH"
                     /locus_tag="ACHOSM_RS01290"
                     /old_locus_tag="pgaptmp_000258"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HGU4556857.1"
                     /translation="MANRKRDAGREALEKKGWWLSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            37465..37914
                     /gene="napB"
                     /locus_tag="ACHOSM_RS01295"
                     /old_locus_tag="pgaptmp_000259"
     CDS             37465..37914
                     /gene="napB"
                     /locus_tag="ACHOSM_RS01295"
                     /old_locus_tag="pgaptmp_000259"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HGU4556858.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            37924..38526
                     /gene="napC"
                     /locus_tag="ACHOSM_RS01300"
                     /old_locus_tag="pgaptmp_000260"
     CDS             37924..38526
                     /gene="napC"
                     /locus_tag="ACHOSM_RS01300"
                     /old_locus_tag="pgaptmp_000260"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HGU4556859.1"
                     /translation="MGNSHRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            38539..39162
                     /gene="ccmA"
                     /locus_tag="ACHOSM_RS01305"
                     /old_locus_tag="pgaptmp_000261"
     CDS             38539..39162
                     /gene="ccmA"
                     /locus_tag="ACHOSM_RS01305"
                     /old_locus_tag="pgaptmp_000261"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HGU4556860.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNSGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            39159..39821
                     /gene="ccmB"
                     /locus_tag="ACHOSM_RS01310"
                     /old_locus_tag="pgaptmp_000262"
     CDS             39159..39821
                     /gene="ccmB"
                     /locus_tag="ACHOSM_RS01310"
                     /old_locus_tag="pgaptmp_000262"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HGU4556861.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            39863..40600
                     /gene="ccmC"
                     /locus_tag="ACHOSM_RS01315"
                     /old_locus_tag="pgaptmp_000263"
     CDS             39863..40600
                     /gene="ccmC"
                     /locus_tag="ACHOSM_RS01315"
                     /old_locus_tag="pgaptmp_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HGU4556862.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            40597..40806
                     /gene="ccmD"
                     /locus_tag="ACHOSM_RS01320"
                     /old_locus_tag="pgaptmp_000264"
     CDS             40597..40806
                     /gene="ccmD"
                     /locus_tag="ACHOSM_RS01320"
                     /old_locus_tag="pgaptmp_000264"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HGU4556863.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVYSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            40803..41282
                     /gene="ccmE"
                     /locus_tag="ACHOSM_RS01325"
                     /old_locus_tag="pgaptmp_000265"
     CDS             40803..41282
                     /gene="ccmE"
                     /locus_tag="ACHOSM_RS01325"
                     /old_locus_tag="pgaptmp_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HGU4556864.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            41279..43222
                     /gene="ccmF"
                     /locus_tag="ACHOSM_RS01330"
                     /old_locus_tag="pgaptmp_000266"
     CDS             41279..43222
                     /gene="ccmF"
                     /locus_tag="ACHOSM_RS01330"
                     /old_locus_tag="pgaptmp_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HGU4556865.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLVGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRILRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            43219..43776
                     /gene="dsbE"
                     /locus_tag="ACHOSM_RS01335"
                     /old_locus_tag="pgaptmp_000267"
     CDS             43219..43776
                     /gene="dsbE"
                     /locus_tag="ACHOSM_RS01335"
                     /old_locus_tag="pgaptmp_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HGU4556866.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            43773..44825
                     /gene="ccmH"
                     /locus_tag="ACHOSM_RS01340"
                     /old_locus_tag="pgaptmp_000268"
     CDS             43773..44825
                     /gene="ccmH"
                     /locus_tag="ACHOSM_RS01340"
                     /old_locus_tag="pgaptmp_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HGU4556867.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(44860..45507)
                     /gene="narP"
                     /locus_tag="ACHOSM_RS01345"
                     /old_locus_tag="pgaptmp_000269"
     CDS             complement(44860..45507)
                     /gene="narP"
                     /locus_tag="ACHOSM_RS01345"
                     /old_locus_tag="pgaptmp_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HGU4556868.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            45827..48418
                     /gene="yejO"
                     /locus_tag="ACHOSM_RS01350"
                     /old_locus_tag="pgaptmp_000270"
     CDS             45827..48418
                     /gene="yejO"
                     /locus_tag="ACHOSM_RS01350"
                     /old_locus_tag="pgaptmp_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HGU4556869.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEWG
                     LAIAVDQKAGGAIKASTRVMEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANEVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRS
                     SVTASAENFITLNTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSIRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNHVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            complement(48522..48598)
                     /locus_tag="ACHOSM_RS01355"
                     /old_locus_tag="pgaptmp_000271"
     tRNA            complement(48522..48598)
                     /locus_tag="ACHOSM_RS01355"
                     /old_locus_tag="pgaptmp_000271"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48562..48564),aa:Pro,seq:ggg)
     gene            complement(48673..50433)
                     /gene="yejM"
                     /locus_tag="ACHOSM_RS01360"
                     /old_locus_tag="pgaptmp_000272"
     CDS             complement(48673..50433)
                     /gene="yejM"
                     /locus_tag="ACHOSM_RS01360"
                     /old_locus_tag="pgaptmp_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HGU4556870.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMTALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(50453..50680)
                     /gene="yejL"
                     /locus_tag="ACHOSM_RS01365"
                     /old_locus_tag="pgaptmp_000273"
     CDS             complement(50453..50680)
                     /gene="yejL"
                     /locus_tag="ACHOSM_RS01365"
                     /old_locus_tag="pgaptmp_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HGU4556871.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            50862..51869
                     /gene="yejK"
                     /locus_tag="ACHOSM_RS01370"
                     /old_locus_tag="pgaptmp_000274"
     CDS             50862..51869
                     /gene="yejK"
                     /locus_tag="ACHOSM_RS01370"
                     /old_locus_tag="pgaptmp_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HGU4556872.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(52008..52292)
                     /gene="rplY"
                     /locus_tag="ACHOSM_RS01375"
                     /old_locus_tag="pgaptmp_000275"
     CDS             complement(52008..52292)
                     /gene="rplY"
                     /locus_tag="ACHOSM_RS01375"
                     /old_locus_tag="pgaptmp_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HGU4556873.1"
                     /translation="MFTINAEVRKEQGKGASRRLRVANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(52417..54177)
                     /gene="radD"
                     /locus_tag="ACHOSM_RS01380"
                     /old_locus_tag="pgaptmp_000276"
     CDS             complement(52417..54177)
                     /gene="radD"
                     /locus_tag="ACHOSM_RS01380"
                     /old_locus_tag="pgaptmp_000276"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU4556874.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPVCGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            54327..55022
                     /gene="rsuA"
                     /locus_tag="ACHOSM_RS01385"
                     /old_locus_tag="pgaptmp_000277"
     CDS             54327..55022
                     /gene="rsuA"
                     /locus_tag="ACHOSM_RS01385"
                     /old_locus_tag="pgaptmp_000277"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HGU4556875.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            55050..56240
                     /gene="bcr"
                     /locus_tag="ACHOSM_RS01390"
                     /old_locus_tag="pgaptmp_000278"
     CDS             55050..56240
                     /gene="bcr"
                     /locus_tag="ACHOSM_RS01390"
                     /old_locus_tag="pgaptmp_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HGU4556876.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            56573..56917
                     /gene="yejG"
                     /locus_tag="ACHOSM_RS01395"
                     /old_locus_tag="pgaptmp_000279"
     CDS             56573..56917
                     /gene="yejG"
                     /locus_tag="ACHOSM_RS01395"
                     /old_locus_tag="pgaptmp_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HGU4556877.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(56921..58510)
                     /gene="yejF"
                     /locus_tag="ACHOSM_RS01400"
                     /old_locus_tag="pgaptmp_000280"
     CDS             complement(56921..58510)
                     /gene="yejF"
                     /locus_tag="ACHOSM_RS01400"
                     /old_locus_tag="pgaptmp_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HGU4556878.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(58512..59537)
                     /gene="yejE"
                     /locus_tag="ACHOSM_RS01405"
                     /old_locus_tag="pgaptmp_000281"
     CDS             complement(58512..59537)
                     /gene="yejE"
                     /locus_tag="ACHOSM_RS01405"
                     /old_locus_tag="pgaptmp_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4556879.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQTDYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(59537..60631)
                     /gene="yejB"
                     /locus_tag="ACHOSM_RS01410"
                     /old_locus_tag="pgaptmp_000282"
     CDS             complement(59537..60631)
                     /gene="yejB"
                     /locus_tag="ACHOSM_RS01410"
                     /old_locus_tag="pgaptmp_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HGU4556880.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSREYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(60632..62446)
                     /gene="yejA"
                     /locus_tag="ACHOSM_RS01415"
                     /old_locus_tag="pgaptmp_000283"
     CDS             complement(60632..62446)
                     /gene="yejA"
                     /locus_tag="ACHOSM_RS01415"
                     /old_locus_tag="pgaptmp_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGU4556881.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            complement(62528..64084)
                     /gene="pdeN"
                     /locus_tag="ACHOSM_RS01420"
                     /old_locus_tag="pgaptmp_000284"
     CDS             complement(62528..64084)
                     /gene="pdeN"
                     /locus_tag="ACHOSM_RS01420"
                     /old_locus_tag="pgaptmp_000284"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGU4556882.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFIHFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(64265..64831)
                     /gene="mepS"
                     /locus_tag="ACHOSM_RS01425"
                     /old_locus_tag="pgaptmp_000285"
     CDS             complement(64265..64831)
                     /gene="mepS"
                     /locus_tag="ACHOSM_RS01425"
                     /old_locus_tag="pgaptmp_000285"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HGU4556883.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            complement(65242..65955)
                     /gene="lpxT"
                     /locus_tag="ACHOSM_RS01430"
                     /old_locus_tag="pgaptmp_000286"
     CDS             complement(65242..65955)
                     /gene="lpxT"
                     /locus_tag="ACHOSM_RS01430"
                     /old_locus_tag="pgaptmp_000286"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HGU4556884.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(65994..66980)
                     /gene="yeiR"
                     /locus_tag="ACHOSM_RS01435"
                     /old_locus_tag="pgaptmp_000287"
     CDS             complement(65994..66980)
                     /gene="yeiR"
                     /locus_tag="ACHOSM_RS01435"
                     /old_locus_tag="pgaptmp_000287"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HGU4556885.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(67098..68564)
                     /gene="yeiQ"
                     /locus_tag="ACHOSM_RS01440"
                     /old_locus_tag="pgaptmp_000288"
     CDS             complement(67098..68564)
                     /gene="yeiQ"
                     /locus_tag="ACHOSM_RS01440"
                     /old_locus_tag="pgaptmp_000288"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGU4556886.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     ERVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGYAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGKAIDVRDPLSDKIRDLVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQISQFGAHQALLNTLKI"
     gene            complement(68787..69359)
                     /gene="yeiP"
                     /locus_tag="ACHOSM_RS01445"
                     /old_locus_tag="pgaptmp_000289"
     CDS             complement(68787..69359)
                     /gene="yeiP"
                     /locus_tag="ACHOSM_RS01445"
                     /old_locus_tag="pgaptmp_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HGU4556887.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            69514..69768
                     /gene="yeiW"
                     /locus_tag="ACHOSM_RS01450"
                     /old_locus_tag="pgaptmp_000290"
     CDS             69514..69768
                     /gene="yeiW"
                     /locus_tag="ACHOSM_RS01450"
                     /old_locus_tag="pgaptmp_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU4556888.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(69765..70946)
                     /gene="setB"
                     /locus_tag="ACHOSM_RS01455"
                     /old_locus_tag="pgaptmp_000291"
     CDS             complement(69765..70946)
                     /gene="setB"
                     /locus_tag="ACHOSM_RS01455"
                     /old_locus_tag="pgaptmp_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HGU4556889.1"
                     /translation="MHNSPALSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            71314..72444
                     /gene="fruB"
                     /locus_tag="ACHOSM_RS01460"
                     /old_locus_tag="pgaptmp_000292"
     CDS             71314..72444
                     /gene="fruB"
                     /locus_tag="ACHOSM_RS01460"
                     /old_locus_tag="pgaptmp_000292"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HGU4556890.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            72444..73382
                     /gene="fruK"
                     /locus_tag="ACHOSM_RS01465"
                     /old_locus_tag="pgaptmp_000293"
     CDS             72444..73382
                     /gene="fruK"
                     /locus_tag="ACHOSM_RS01465"
                     /old_locus_tag="pgaptmp_000293"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGU4556891.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            73399..75090
                     /gene="fruA"
                     /locus_tag="ACHOSM_RS01470"
                     /old_locus_tag="pgaptmp_000294"
     CDS             73399..75090
                     /gene="fruA"
                     /locus_tag="ACHOSM_RS01470"
                     /old_locus_tag="pgaptmp_000294"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HGU4556892.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            75514..76455
                     /gene="psuK"
                     /locus_tag="ACHOSM_RS01475"
                     /old_locus_tag="pgaptmp_000295"
     CDS             75514..76455
                     /gene="psuK"
                     /locus_tag="ACHOSM_RS01475"
                     /old_locus_tag="pgaptmp_000295"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HGU4556893.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLTAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSFLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            76443..77381
                     /gene="psuG"
                     /locus_tag="ACHOSM_RS01480"
                     /old_locus_tag="pgaptmp_000296"
     CDS             76443..77381
                     /gene="psuG"
                     /locus_tag="ACHOSM_RS01480"
                     /old_locus_tag="pgaptmp_000296"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HGU4556894.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            77475..78725
                     /gene="psuT"
                     /locus_tag="ACHOSM_RS01485"
                     /old_locus_tag="pgaptmp_000297"
     CDS             77475..78725
                     /gene="psuT"
                     /locus_tag="ACHOSM_RS01485"
                     /old_locus_tag="pgaptmp_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU4556895.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(78796..79494)
                     /gene="yeiL"
                     /locus_tag="ACHOSM_RS01490"
                     /old_locus_tag="pgaptmp_000298"
     CDS             complement(78796..79494)
                     /gene="yeiL"
                     /locus_tag="ACHOSM_RS01490"
                     /old_locus_tag="pgaptmp_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HGU4556896.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALSMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLTKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            79624..80565
                     /gene="rihB"
                     /locus_tag="ACHOSM_RS01495"
                     /old_locus_tag="pgaptmp_000299"
     CDS             79624..80565
                     /gene="rihB"
                     /locus_tag="ACHOSM_RS01495"
                     /old_locus_tag="pgaptmp_000299"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HGU4556897.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            80665..81915
                     /gene="nupX"
                     /locus_tag="ACHOSM_RS01500"
                     /old_locus_tag="pgaptmp_000300"
     CDS             80665..81915
                     /gene="nupX"
                     /locus_tag="ACHOSM_RS01500"
                     /old_locus_tag="pgaptmp_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU4556898.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(81971..83059)
                     /gene="yeiI"
                     /locus_tag="ACHOSM_RS01505"
                     /old_locus_tag="pgaptmp_000301"
     CDS             complement(81971..83059)
                     /gene="yeiI"
                     /locus_tag="ACHOSM_RS01505"
                     /old_locus_tag="pgaptmp_000301"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU4556899.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(83062..83919)
                     /gene="nfo"
                     /locus_tag="ACHOSM_RS01510"
                     /old_locus_tag="pgaptmp_000302"
     CDS             complement(83062..83919)
                     /gene="nfo"
                     /locus_tag="ACHOSM_RS01510"
                     /old_locus_tag="pgaptmp_000302"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HGU4556900.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(83993..85042)
                     /gene="yeiH"
                     /locus_tag="ACHOSM_RS01515"
                     /old_locus_tag="pgaptmp_000303"
     CDS             complement(83993..85042)
                     /gene="yeiH"
                     /locus_tag="ACHOSM_RS01515"
                     /old_locus_tag="pgaptmp_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGU4556901.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            85141..86022
                     /gene="yieE"
                     /locus_tag="ACHOSM_RS01520"
                     /old_locus_tag="pgaptmp_000304"
     CDS             85141..86022
                     /gene="yieE"
                     /locus_tag="ACHOSM_RS01520"
                     /old_locus_tag="pgaptmp_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HGU4556902.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            86227..87696
                     /gene="lysP"
                     /locus_tag="ACHOSM_RS01525"
                     /old_locus_tag="pgaptmp_000305"
     CDS             86227..87696
                     /gene="lysP"
                     /locus_tag="ACHOSM_RS01525"
                     /old_locus_tag="pgaptmp_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HGU4556903.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            87990..89981
                     /gene="cirA"
                     /locus_tag="ACHOSM_RS01530"
                     /old_locus_tag="pgaptmp_000306"
     CDS             87990..89981
                     /gene="cirA"
                     /locus_tag="ACHOSM_RS01530"
                     /old_locus_tag="pgaptmp_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HGU4556904.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSFNYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(90013..90849)
                     /gene="yeiG"
                     /locus_tag="ACHOSM_RS01535"
                     /old_locus_tag="pgaptmp_000307"
     CDS             complement(90013..90849)
                     /gene="yeiG"
                     /locus_tag="ACHOSM_RS01535"
                     /old_locus_tag="pgaptmp_000307"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU4556905.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDEKFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            91107..91775
                     /gene="folE"
                     /locus_tag="ACHOSM_RS01540"
                     /old_locus_tag="pgaptmp_000308"
     CDS             91107..91775
                     /gene="folE"
                     /locus_tag="ACHOSM_RS01540"
                     /old_locus_tag="pgaptmp_000308"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGU4556906.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            91792..92949
                     /gene="yeiB"
                     /locus_tag="ACHOSM_RS01545"
                     /old_locus_tag="pgaptmp_000309"
     CDS             91792..92949
                     /gene="yeiB"
                     /locus_tag="ACHOSM_RS01545"
                     /old_locus_tag="pgaptmp_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HGU4556907.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            93091..94131
                     /gene="galS"
                     /locus_tag="ACHOSM_RS01550"
                     /old_locus_tag="pgaptmp_000310"
     CDS             93091..94131
                     /gene="galS"
                     /locus_tag="ACHOSM_RS01550"
                     /old_locus_tag="pgaptmp_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HGU4556908.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGSLTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            94411..95409
                     /gene="mglB"
                     /locus_tag="ACHOSM_RS01555"
                     /old_locus_tag="pgaptmp_000311"
     CDS             94411..95409
                     /gene="mglB"
                     /locus_tag="ACHOSM_RS01555"
                     /old_locus_tag="pgaptmp_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HGU4556909.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNAKKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKYNLAEFSKK"
     gene            95470..96990
                     /gene="mglA"
                     /locus_tag="ACHOSM_RS01560"
                     /old_locus_tag="pgaptmp_000312"
     CDS             95470..96990
                     /gene="mglA"
                     /locus_tag="ACHOSM_RS01560"
                     /old_locus_tag="pgaptmp_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HGU4556910.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            97006..98016
                     /gene="mglC"
                     /locus_tag="ACHOSM_RS01565"
                     /old_locus_tag="pgaptmp_000313"
     CDS             97006..98016
                     /gene="mglC"
                     /locus_tag="ACHOSM_RS01565"
                     /old_locus_tag="pgaptmp_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HGU4556911.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(98087..99322)
                     /gene="preA"
                     /locus_tag="ACHOSM_RS01570"
                     /old_locus_tag="pgaptmp_000314"
     CDS             complement(98087..99322)
                     /gene="preA"
                     /locus_tag="ACHOSM_RS01570"
                     /old_locus_tag="pgaptmp_000314"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HGU4556912.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(99316..100554)
                     /gene="preT"
                     /locus_tag="ACHOSM_RS01575"
                     /old_locus_tag="pgaptmp_000315"
     CDS             complement(99316..100554)
                     /gene="preT"
                     /locus_tag="ACHOSM_RS01575"
                     /old_locus_tag="pgaptmp_000315"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HGU4556913.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSAQELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTINTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT        24300 a        24740 c        27171 g        24608 t
ORIGIN      
        1 tataaaatga tggtcagatt aatatgcgga ttatctttct acgcaaggag tatttatctc
       61 tactcccgtc aatgattgca tctcttttct ctgctaacgg tgtcgcggcg gccattgatt
      121 tatgccaggg atatgatatc aaagcgagtt gtcacgccag caggcaaagc ctttcaggca
      181 ttacgcagga ctggagcatc gccgatggac aatggctggt tttttcggat atgaccaata
      241 atgccagcgg aggagccgta tttttgcaac aaggagcgga atttacatta tcgccagaaa
      301 atgaaactgg aatgactttg tttgccaata acaccgttac aggagaatat aataacggcg
      361 gggccatatt tgctaaagaa aactcaacgc tgaatcttac tgatgttatt ttttccggta
      421 acgtcgcagg cggctatggt ggcgcaatct attcttctgg tactaacgat actggtgccg
      481 tcgatttacg tgtcactaac gccatgtttc gcaataacat cgctaatgat ggcaaaggtg
      541 gcgcaattta taccattaat aatgacgttt atttaagtga tgttattttt gataacaacc
      601 aggcatatac atcaacaagt tacagtgatg gcgatggcgg ggcaatcgat gttaccgata
      661 ataatagcga cagcaagcat ccttcaggtt atacgataat aaataacacc gcctttacaa
      721 ataacactgc cgaaggttat ggcggggcga tatataccaa tagcgcgacg gctccctatc
      781 ttattgatat ttctgttgat gacagctaca gccagaacgg aggcgtgtta gtcgatgaga
      841 acaacagcgc agcaggctat ggagatggtc cttccactgc ggcgggtggc tttatgtatc
      901 tcggcttaag tgaagttacc tttgatattg ccgacggaaa aacgctggtt attggcaata
      961 cagagaatga cggagctgtt gactctattg ctggtaccgg gttgatcacc aaaacaggtt
     1021 ccggcgatct ggtacttaat gcagataaca atgactttac tggtgagatg cagattgaaa
     1081 acggtgaagt taccctgggt cgcagcaact cactgatgaa tgtcggcgat acgcattgcc
     1141 aggacgatcc gcaagactgc tacggtctga cgatagggag tattgataag taccagaatc
     1201 aggcagagct aaacgttggc tccacccaac aaacctttgt gcactcattg acgggctttc
     1261 agaatggcac tttaaatatc gatgctggtg gcaatgttac tgttaatcaa ggtagttttg
     1321 ctggcaccat cgaaggtgct ggtcagctca ccattgcgca aaacggcagc tacgtgctgt
     1381 cgggggcgca gtcgatggcg ctaaccggcg atatagtcgt tgatgatagt gcggtgcttt
     1441 cgctggaagg cgacgcggca gatctcgccg ctctccagga cgatccgcag tcgatcgtgt
     1501 taaacggcgg tgtgctcgat ctctctgatt tctccacctg gcagagcggc acatcataca
     1561 atgatggcct tgaagtcagt ggcagcagcg gaacggttat cggcagtcag gatgtggtag
     1621 atcttgcagg tggcgacaat ttgcatatcg gcggcgacgg gaaagatggc gtctacgtgg
     1681 tggtcgatgc gagcgacggg caggtaagtc tggcaaacaa taatagttat ttgggcacaa
     1741 cacaaatcgc ctccggtacg ctgatggtga gcgacaactc gcagcttgga gatacccact
     1801 ataaccgcca ggttatcttt accgataagc aacaagaaag cgtgatggag attactgcca
     1861 atgtcgatac tcgctctacg acgactgagc atgggcgtga tattgaaatg cgcgccgacg
     1921 gtgaagtggc agttgatgcg ggggtagaca cgcagtgggg cgcactgatg gctgacagca
     1981 gcgggcagca tcaggatgag ggtagcacat ttactaaaac gggggcgggt acgctggagc
     2041 tgaccgccag cggtacaacg cagtcggcag tacgtgtcga agaaggcacc ctgaaaggcg
     2101 atgttgcgga tatctttcct tatgcttcgt cactgtgggt cggtgatggg gcgacgttcg
     2161 ttactggcgc ggatcaggat attcagtcta tcgatgctac ttccagcggc actatcgata
     2221 tcagcgatgg tacggttttg cgtctgaccg ggcaggatac ttccgtcgcc cttaatgcct
     2281 cattgtttaa cggcgatggg acgctggtga atgctaccga tggcgtgacg ttgacaggtg
     2341 agcttaatac caaccttgaa actgacagcc tgacctatct tgccgacgtg acggttaatg
     2401 gtgatctgac caatacgtcc ggtgcggtta gcctgcaaaa tggcgtcgct ggcgatacgc
     2461 tgacggtaaa cggtgattat accggcggcg gtacgctact gttcgacagc gaattaaacg
     2521 gcgatgactc ggtaagcgat caattggtga tgaacggtaa tactgctggc aacacaactg
     2581 tggtggttaa ctccattaca gggattggtg agccgacatc gacaggcatt aaagtggttg
     2641 atttcgcagc tgatcccacg cagtttcaaa acaatgcaca gttcagtctg gctggcagtg
     2701 gctacgtcaa catgggagcg tatgactaca cgctagtgga agataacaac gactggtatc
     2761 tgcgatcgca agaagtaacg ccgccatcgc cacctgatcc agacccggac ccaacacccg
     2821 atcctgaccc aacacccgat ccaatccccg cttaccagcc ggtgctgaat gccaaagttg
     2881 gcggttatct caataatctg cgggcggcaa atcaggcgtt tatgatggag cgacgcgatc
     2941 atgcaggtgg caatggtcag acgctgaatt tacgtgttat cggcggacgt tatcattaca
     3001 cggcggcagg gcaactggct cagcatgaag acacttctac ggtgcagctt agcggtgacc
     3061 tgtttagcgg gcgctggggc gatgatggcg agtggatgct tggggcggtt ggtggctaca
     3121 gtgataatca gggcgagagc cgctcgaata tgaccggaac tcgcgccgat aaccagaacc
     3181 acggttatgc cgttgggctg acctcaagct ggtatcagca cggtaatcag aagcaggggg
     3241 cctggctgga tagctggctg caatacgcgt ggtttaacaa tgatgtttcc gaacaagacg
     3301 atggcacaga tcattaccac tcgtcgggga ttatcgcctc gctggaagcc gggtatcagt
     3361 ggttaccggg gcgtggtgtg gtgattgaac cgcaggcgca ggtgatttat cagggcgtgc
     3421 agcaggatga ttttaccgcc gctaaccatg cgcgcgtgtc acaatcgcag ggggatgata
     3481 ttcagacgcg gctgggttta cacagcgaat ggcgtaccgc ggttggtgtc acaccaacat
     3541 tagatctgaa ttattatcac gatccccatg cgacggaaat tgaagaagat ggcaggacta
     3601 tcagtgacga tgcggtgaag cgacggggtg aaataaaagt gggagtcacg ggcaatatca
     3661 gtcagcgagt gtccctgcgc ggcagcgtgg cgtggcagaa agggagtgat gattttgccc
     3721 agacggcagg gtttttgtcg atgacggtga aatggtaatc actgtctagt cggcacggtt
     3781 gccggatgcg gcgtaaacgc cttatccggc ctacggggag catttgtagg cctgataaga
     3841 cgcggcaagc gtcgcatcag gcattggctc ggccaaacaa caacctcact tattctgcgt
     3901 gtgcagcata tagttcacat ccacgccggg gccgagttta aaagtattag tgagcgggtt
     3961 gtaatgtagc ccggttatat gtcgctcttt aaggcttgtc tgatctaccc agccaagcag
     4021 ttctgccggt ttaataaact tcttcacatc atgcgtgcct ttgggcacca tgcgcaaaat
     4081 atattccgcg ccaaccaccg ccatcagcca tgacttgccg ttgcggttaa gcgtcgagaa
     4141 aaagacatcg ccgcctggtt tcaccagttg cgcacaggct ctgaccactg actgcggatc
     4201 ggggacgtgc tccagcatct ccatgcaggt caccacatca tactgcccgg catgttttgc
     4261 cgcgtgctct tccacggttt cctgcacgta atccacctga atgccgcttt ccagcgcgtg
     4321 cagctttgcc acctgcaatg gctcaaagcc catatccaga ccggtcaccg tcgcgccttc
     4381 gcgcgccata ctctcggcca aaatgccgcc gccgcaaccg acatcgagca cctttttgcc
     4441 gaataaaccg ccagcacgct cggcaatata gcccagacgc agcgggttaa tgcggtgcag
     4501 cggtttgaac tcaccttcaa gatcccacca gcgggaggcg acggcttcaa atttagcgat
     4561 ctcttcgtgg tctacgttat ggttttccgg cgatttttcg gcattcattg gcacttctac
     4621 tccgtaattg gcaagacaaa cgagtatatc aggcattgga tgtgaataaa gcgtataggt
     4681 ttacctcaaa ctgcgcggct gtgttataat ttgcgacctt tgaatccggg atacagtaga
     4741 gggatagcgg ttagatgagc gaccttgcga gagaaattac accggtcaac attgaggaag
     4801 agctgaagag ctcctatctg gattatgcga tgtcggtcat tgttggccgt gcgctgccag
     4861 atgtccgaga tggcctgaag ccggtacacc gtcgcgtact ttacgccatg aacgtactag
     4921 gcaatgactg gaacaaagcc tataaaaaat ctgcccgtgt cgttggtgac gtaatcggta
     4981 aataccatcc ccatggtgac tcggcggttt atgacacgat cgtccgtatg gcgcagccat
     5041 tctcgctgcg ttacatgctg gtagacggtc agggtaactt cggttccatc gacggcgact
     5101 ctgcggcggc aatgcgttat acggaaatcc gtctggcgaa aattgcccat gaactgatgg
     5161 ccgatctcga aaaagagacg gtcgattttg ttgataacta tgacggcacg gaaaaaattc
     5221 ccgacgtcat gccaaccaaa attcctaacc tgctggtgaa cggttcttcc ggtatcgccg
     5281 taggtatggc aaccaacatc ccgccgcaca acctgacgga agtcatcaac ggttgtctgg
     5341 cgtatatcga tgatgaagac atcagcattg aagggctgat ggaacacatc ccggggccgg
     5401 acttcccgac ggcggcaatc attaacggtc gtcgcggtat tgaagaagct taccgtaccg
     5461 gtcgcggcaa ggtgtatatc cgcgctcgcg cagaagtgga agttgacgcc aaaaccggtc
     5521 gtgaaaccat tatcgtccac gaaattccgt atcaggtaaa caaagcgcgc ctgatcgaga
     5581 agattgcgga actggtaaaa gaaaaacgcg tggaaggcat cagcgcgctg cgtgacgagt
     5641 ctgacaaaga cggtatgcgc atcgtgattg aagtgaaacg cgatgcggtc ggtgaagtgg
     5701 tgctcaacaa cctctactcc cagacccagt tgcaggtttc tttcggtatc aacatggtgg
     5761 cattgcacca tggtcagccg aagatcatga acctgaaaga catcatcgcg gcatttgttc
     5821 gtcaccgccg tgaagtggtg acccgtcgta ctattttcga actgcgtaaa gctcgcgatc
     5881 gtgctcatat ccttgaagca ttagccgtgg cgctggcgaa catcgacccg atcatcgaac
     5941 tgatccgtca tgcgccgacg cctgcagaag cgaaaactgc gctggttgct aatccgtggc
     6001 agctgggcaa cgttgccgcg atgctcgaac gtgctggcga cgatgctgcg cgtccggaat
     6061 ggctggagcc agagttcggc gtgcgtgatg gtctgtacta cctgaccgaa cagcaagctc
     6121 aggcgattct ggatctgcgt ttgcagaaac tgaccggcct tgagcacgaa aaactgctcg
     6181 acgaatacaa agagctgctg gatcagatcg cggaactgtt gcgtattctt ggtagcgccg
     6241 atcgtctgat ggaagtgatc cgtgaagagc tggagctggt tcgtgaacag ttcggtgaca
     6301 aacgtcgtac tgaaatcacc gccaacagcg cagacatcaa cctggaagat ctgatcaccc
     6361 aggaagatgt ggtcgtgacg ctctctcacc agggctacgt taaatatcag ccgctttctg
     6421 aatacgaagc gcagcgtcgt ggcgggaaag gtaaatctgc cgcacgtatt aaagaagaag
     6481 actttatcga ccgactgctg gtggcgaaca ctcacgacca tattctgtgc ttctccagcc
     6541 gtggtcgcgt ctattcgatg aaagtttatc agttgccgga agccactcgt ggcgcgcgcg
     6601 gtcgtccgat cgtcaacctg ctgccgctgg agcaggacga acgtatcact gcgatcctgc
     6661 cagtgaccga gtttgaagaa ggcgtgaaag tcttcatggc gaccgctaac ggaaccgtga
     6721 agaaaactgt cctcaccgag ttcaaccgtc tgcgtaccgc cggtaaagtg gcgatcaaac
     6781 tggttgacgg cgatgagctg atcggcgttg acctgaccag cggcgaagac gaagtaatgc
     6841 tgttctccgc tgaaggtaaa gtggtgcgct ttaaagagtc ttctgtccgt gcgatgggct
     6901 gcaacaccac cggtgttcgc ggtattcgct taggtgaagg cgataaagtc gtctctctga
     6961 tcgtgcctcg tggcgatggc gcaatcctca ccgcaacgca aaacggttac ggtaaacgta
     7021 ccgcagtggc ggaataccca accaagtcgc gtgcgacgaa aggggttatc tccatcaagg
     7081 ttaccgaacg taacggttta gttgttggcg cggtacaggt agatgactgc gaccagatca
     7141 tgatgatcac cgatgccggt acgctggtac gtactcgcgt ttcagaaatc agcatcgtgg
     7201 gccgtaacac ccagggcgtg atcctcatcc gtactgcgga agatgaaaac gtagtgggtc
     7261 tgcaacgtgt tgctgaaccg gttgacgagg aagatctcga taccatcgac ggcagtgccg
     7321 cggaagggga cgatgaaatc gctccggaag tggacgttga cgacgagcca gaagaagaat
     7381 aattttactt cttcatgcca aaagggagct atctcccttg tttgaattga aaagtccagg
     7441 ctgcaaagtc tgggcttttg tcgtattagg gcacggtaaa gtttggctgt gtccgtaaaa
     7501 aatggctggc tatacacaag gaatgtggca atgagtggtg aaaaaaaggc gaaaggctgg
     7561 cggttctatg gtcttgtagg ttttggcgca atagcactgc tttccgctgg cgtctgggcg
     7621 ttgcaatatg ctggcagtgg gccagaaaaa acgttgtcgc cgctggtggt gcacaacaat
     7681 ctgcaaatcg atctcaatga gccggacctc tttctcgaca gcgactctct gagccagctt
     7741 cccaaagatc tcctcaccat tccgtttctc cacgatgttc tgagcgaaga tttcgttttc
     7801 tattatcaga atcatgccga tcgtctgggc attgaaggca gcattcgtcg tattgtctat
     7861 gaacacgatc tcacgctgaa agataagctc ttttcgtcac tcttagatca gcccgcgcag
     7921 gcagcgctgt ggcacgataa acaaggccat ctttcacatt atatggtgct gatccagcgc
     7981 agtggtttaa gcaaactgct ggagccattg ttgtttgccg ctaccagcga cagccagtta
     8041 agcaaaacgg aaatcagtag catcaagata aatagtgaaa ctgttcctgt ttatcagttg
     8101 cgctataacg gcaataacgc tctgatgttc gcgacatatc aggacaagat gctagtgttt
     8161 tccagcacgg atatgctgtt taaagatgat cagcaggata ccgaagccac ggcgatcgca
     8221 ggtgatttgt tgagcggcaa aaaacgctgg caagcaagct ttggcctgga agagcgtgct
     8281 gctgaaaaaa cgccagtacg ccagcgcatc gtggtcagcg ccaggttgct gggttttggc
     8341 taccagcggt taatgccttc ttttgctggc gtacgcttcg aaatgggtaa cgacggctgg
     8401 cacagttttg tggcgttaaa tgatgaatcc gccagcgtag ataccagttt cgattttacg
     8461 ccggtctgga acagtatgcc tgccggggcc agcttctgtg tggcggtgcc gtattcacac
     8521 ggtattgccg aagagatgct ttcgcacatc agccaggaaa acgacaagct gaatggggcg
     8581 ttagacggtg ccgcggggct gtgctggtat gaagactcaa aattgcaaac cccgctgttt
     8641 gtcggtcagt ttgatggcac tgccgaacag gcgcaattgc cagggaaact gtttacgcaa
     8701 aatattggtg cgcacgaaag caaagcgcca gaaggtgttt tgccgataag ccagactcag
     8761 cagggcgaag cgcaaatctg gcgtcgcgaa gtgagttccc gatacggtca gtatccgaaa
     8821 gcgcaggcgg cgcaacccga tcaattaatg tcggattatt ttttccgcgt gtcgctggcg
     8881 atgcaaaaca aaacgctgct tttctccctc gatgacacgc tggttaataa cgccctgcaa
     8941 acactgaata aaaaccgccc ggcaatggtg gatgtaatac ccactgatgg catcgttccg
     9001 ctctatatca atccacaagg cgtggcgaaa ctgctgcgta acgaaacgtt gaccagtctg
     9061 ccgaagaatc tcgaaccggt tttttataac gccgcacaaa ctttattaat gccgaagctg
     9121 gacgctttat ctcaacaacc gcgttatgtc atgaagctgg cccagatgga acccggtgcc
     9181 gcctggcagt ggctacccat aacctggcag ccgctatgag gcacgggctg ctggcgctga
     9241 tttgctggct gtgttgtgtt gttgcccata gcgaaatgct gaatgtcgaa caatccgggc
     9301 tgtttcgcgc ctggtttgta cgcattgcac aagaacagct ccgccagggg ccaagtccac
     9361 gctggtatca gcaggattgt gcgggcctgg tgcgatttgc ggcgaacgag acgctgaaag
     9421 ttcacgatag taaatggcta aaaagtaacg gtttctctag ccagtatttg ccgccagaga
     9481 tgacgctaac acctggacag cgtcaactgg cgcaaaactg gaatcagggg aacgggaaaa
     9541 ccggccccta cgtgaccgcg attaatttga ttcagtacaa cagccagttt attggccagg
     9601 acataaacca ggcgctgcct ggcgatatga tttttttcga tcagggcgat gcccagcact
     9661 taatggtctg gatggggcgt tacgtcatct accacaccgg aagcgccacg aaaactgaca
     9721 acggaatgcg cgcagtcagt ctgcaacaac ttatgacatg gaaggacacc cgatggatac
     9781 ccaacgattc caatcccaat ttcattggca tttatcgttt aaattttctg gcgcgatagc
     9841 agcgtgcttg tccctgtctc ttgtgggagc agggcttgct aatgctgatg attcgcttcc
     9901 ttccagtaac tatgcgccgc ccgccggggg aacattcttt ttgcttgctg acagcagttt
     9961 tagcagcagt gaagaggcga aagtgcgact ggaagcgccg gggcgtgatt atcggcgcta
    10021 tcagatggaa gagtacggcg gcgtggacgt tcgcctgtat cgtattcctg acccgatggc
    10081 atttttgcgc cagcagaaaa acctgcatcg cattgtggtg caaccgcaat atctgggcga
    10141 cgggctgaac aatacgctga cctggctgtg ggataactgg tacggcaaat ctcgccgcgt
    10201 aatgcagcgt actttctctt ctcagtcacg gcagaatgtg actcaggcat tacccgaatt
    10261 acatctcggc aatgccatta ttaaaccttc ccgttatgta cagaacaacc agttttcccc
    10321 gctgaaaaaa tatcccttgg tggaacagtt ccgttatcca ctatggcagg ctaaaccggt
    10381 cgagccgcag caaggggtaa aactggaagg cgcatccagc aatttcatct cgccgcagcc
    10441 gggcaacatc tatattcctc tcggcaaaca agagccggga ctgtacctcg tcgaggcgat
    10501 ggtaggtggg tatcgggcga cgacggtggt gtttgtttcc gataccgtgg cgcttagcaa
    10561 agtgtcaggc aacgagcttc tggtgtggac cgcgggtaaa aaacagggtg aagcgaagcc
    10621 cggctcagag atcttgtgga ctgacggtct tggcgtgatg acccgcgggg tgaccgatga
    10681 cagcggtact ttgcagttac aacatatatc gccagaacgt tcatacattc tgggtaagga
    10741 tgctgaaggc ggcgtttttg tctccgagaa cttcttctac gaaagtgaaa tctacaccac
    10801 ccgcttgtat atttttaccg atcgcccgct atatcgcgca ggcgatcgtg tcgatgttaa
    10861 agtgatgggg cgcgagttcc acgatccgtt gcattcatcc cccatcgtca gcgccccggc
    10921 gaagctttcg gtgctggacg ctaacggcag tctgttgcaa accgtcaatg tcacgctgga
    10981 tgcgcgcaat ggcgggcagg gaagtttccg cctgccagaa aatgccgtag ccggaggtta
    11041 tgagttacgt cttgcttacc gcaatcaggt ctatagcagc agttttcgcg tggcaaacta
    11101 catcaagcca catttcgaga ttggtttagc tctcgacaaa aaagagttca aaactggcga
    11161 agcggtcagc ggcaaactgc aactgcttta cccggatggt gagccggtaa aagatgcccg
    11221 cgtgcagtta agtttgcgcg ctcagcaatt atcaatggtc ggtaacgatt tgcgttatgc
    11281 cggacgtttt cccgtgtcgc tggaaggcag cgaaacggtg tccgacgcca gcggtcatgt
    11341 ggcgttaaat ctccccgccg ccgataaacc gagccgctat ttgttaaccg tctccgccag
    11401 tgacggcgcg gcgtatcgcg tcaccaccac caaagagatc ctcattgaac gcggcctggc
    11461 gcattactct ttaagtaccg ccgcacaata cagtaatagc ggcgagctac cgtcaggcgg
    11521 caaatccttt accgtcaatt tcgctaaacc tggcaactac aatctgacat tacgcgataa
    11581 agacggctta attctcgctg ggttaagtta tgccgtcagc ggtaagggca gcacggcgca
    11641 tacgggtacg gtagatatcg tggcggataa aacgctgtac cagccaggcg aaaccgcgaa
    11701 gatgctgatt acctttccgg agccaattga tgaagcatta ttgacgctgg aacgcgatcg
    11761 cgtggaacag cagtcgctgc tttcgcatcc ggcaaactgg ctaacgctac aacgtttaaa
    11821 cgatacccag tatgaagccc gggttccagt gagcaattcc tttgcgccta acatcacttt
    11881 ttcggtgctg tatacccgta atggtcagta cagttttcag aacgccggga tcaaagttgc
    11941 cgttcctcag cttgatatcc gggtgaaaac ggacaaaacc cattaccagc ttggtgaact
    12001 ggtcaatgtc gaattaacct cgtcgctgaa aggtaaacct gtttctgcgc agctaacggt
    12061 aggcgtggtc gatgaaatga tctacgcgct gcaaccagaa atcgcgccga atatcggcaa
    12121 atttttctat ccgctggggc gtaacaatgt gcgtaccagc tccagtctgt cgtttatcag
    12181 ctacgaccag gcgctctcca gcgagccggt tgcgcctggc gcaactaacc gcagcgagcg
    12241 gcgagtaaaa atgcttgaac gtccacggcg tgaagaggtg gataccgcgg catggatgcc
    12301 gtcactcaca accgataaac aaggcaaagc gtatttcacg ttcctgatgc ctgattcgtt
    12361 aacccgctgg cgtatcaccg cgcgtgggat gaacggcgac gggctggtcg ggcaggggcg
    12421 tgcttatctg cgttcggaaa aaaatctcta catgaagtgg agtatgccaa cggtgtatcg
    12481 cgtgggcgac aaaccggcgg caggactgtt tatcttcagt cagcaggata acgaaccggt
    12541 ggcgctggtg actaaatttg caggcgctga gatgcgccag acgctgacgc tgcacaaagg
    12601 ggcgaattat atttcgctga cgcagaatat tcagcaatct ggcttgttaa gtgcagaact
    12661 gcaacaaaat gggcaagtgc aggacagcat tagcacaaaa ctgtcttttg tggacaacag
    12721 ctggcccgtt gaacagcaga aaaatgtcat gctcggcggt ggcgagaacg cgctgatgtt
    12781 gcccgagcag gcgagtaata tccggctaca aagtagtgaa acgccgcagg agattttccg
    12841 caacaatctt gatgcgttag tcgatgaacc gtggggtggg gtgatcaaca ccggtagccg
    12901 tctgatcccg ctcagtctcg cctggcgttc gcttgccgat catcaaagtg ccgccgctaa
    12961 cgacattcgt cagatgattc aggataaccg tctgcggctg atgcaactgg cggggcccgg
    13021 agcgcgcttt acctggtggg gtgaagatgg caatggtgac gccttcctta cggcatgggc
    13081 atggtacgcc gactggcagg ccagccaggc gctcggcgta acgcaacaac cggaatactg
    13141 gcagcatatg ctcgacagtt atgccgagca ggcggataac atgccgttat tgcatcgggc
    13201 gctggtgctg gcgtgggcac aggagatgaa tctgccgtgc aaaacgttgt tgaaagggtt
    13261 ggatgaagct atcgcccggc gcggaactaa agatgaagat ttctctgagg aagacatccg
    13321 cgatatcaat gacagcctga tcctcgatac accggaatct ccactggcag atgcggtggc
    13381 aaacgtctta accatgacgt tgctgaaaaa agcgcagttg aagtccacgg tgatgccaca
    13441 ggttcagcaa tatgcgtggg ataaagcggc aaacagcaat cagccgctgg cgcacacggt
    13501 tgtgctgctc aatagcggtg gtgacgctac ccagacggcc gctattttaa gtggtttgac
    13561 cgctgagcaa tccactattg agcgcgcgct ggccatgaac tggctggcga aatatatggc
    13621 gacaatgcct ccagttgttt tgcctgcgcc tgcgggcgca tgggctaaac ataagttaac
    13681 tggagggggc gaagactggc gttgggttgg tcagggtgtg ccggacattc tctcttttgg
    13741 tgacgaatta tcgccgcaaa atgtgcaggt ccgctggcgt gaaccggcaa aaacggctca
    13801 acaaagtaac attccggtga ccgttgaacg ccagttgtat cggcttatcc ccggtgaaga
    13861 agagatgagc tttactctgc agccggtgac cagcaatgag attgacagcg atgcgctgta
    13921 tctcgatgaa attacgctta ccagcgagca ggatgcagtt ctgcgctatg gtcaggtgga
    13981 agtaccgctc ccgccgggag ccgacgttga gcgcacaaca tggggcattt tagtcaataa
    14041 acccaacgcc gcgaaacagc aggggcaatt gctggaaaaa gcgcgtaatg aaatgggcga
    14101 actggcctat atggtgccgg tgaaagaact gacgggaacg gtcactttcc gccatttgct
    14161 gcgcttctcg caaaaagggc aattcgttct gcctcctgct cgttatgtgc gttcctatgc
    14221 acctgcgcag caaagtgttg cggcagggag tgaatggacc gggatgcagg tgaaataagt
    14281 gaactggcgc agaatcgtct ggctattggc gttggttact ctgccaacgc ttgcggagga
    14341 gacgccgtta caactggtgc tgcgcggtgc gcagcacgat caactgtatc agctgtcgtc
    14401 atcaggcgtg accaaagtct cagcattacc tgactcgctc accacgccgt tagggagttt
    14461 gtggaaactg tatgtttacg cctggctgga ggatacccat caaccggagc agccctacca
    14521 gtgtcgcgga aactccccgg aagaggttta ttgttgtcag gctggggaga gcataacgcg
    14581 cgataccgcg ctggtacgtt cctgtgggct ctattttgcg ccgcagcggt tgcatattgg
    14641 cgcagatgtg tgggggcaat actggcaaca gcgtcaggca cctgcgtggt tagcgtctct
    14701 tacaacgctg aagccagaaa tctcggtaac ggttaaatcg ctgcttgatt cattagcgac
    14761 gttacctgcg caaaacaaag ctcaggaagt cttactggat gtggtgctgg acgaggcgaa
    14821 aatcggtgtc gcatccatgc tcggtagtcg ggtacgggtg aaaacctgga gctggtttgc
    14881 cgatgataaa caggaaatcc gtcagggtgg ttttgccggc tggttaaccg atggtacacc
    14941 gctgtgggtt actggcagtg gcaccagcaa aacggtacta acccgttatg cgacggtgct
    15001 taatcgggtt ttgccagtgc cgacgcaggt tgccagtggg caatgtgtag aggtggagct
    15061 gtttgcccgc tatccgctga agaaaatcac cgcggaaaag agtacgacgg cagtaaaacc
    15121 cggcgtactt aatgggcgct atcgtgtgac gtttactaat ggtaaccaca ttacgtttgt
    15181 cagccacggt gagacgacct tactgagcga aaagggcaaa ctgaaattgc agtcgcatct
    15241 cgatcgtgaa gagtacgttg ccagagttct ggatcgcgag gcgaaaagta cgccccctga
    15301 ggcagcgaaa gccatgaccg tggcgattcg cactttttta cagcaaaacg ccaatcggga
    15361 aggcgattgc ctgaccatcc ccgacagcag cgccacgcag cgtgtttctg cttcgccagc
    15421 gaccacgggg gcgcgaacga tgaccgcctg gacgcaggat ctcatctatg caggcgatcc
    15481 ggttcattat catggcagtc gagctaccga agggacactt tcctggcggc aagccacggc
    15541 ccaggcgggg cagggagagc gttacgacca gatactcgcg tttgcttatc ccgacaacag
    15601 ccttagccgc tggggagcgc cacgctcaac ctgccagtta ttacccaaag cgaaagcctg
    15661 gctggcgaaa aaaatgccgc agtggcggcg tatattacaa ggcgagacgg ggtacaacga
    15721 accagacgtg tttgcggtct gtcgtttagt ctcaggtttc ccctataccg atcggcagca
    15781 gaaacggctg tttattcgta acttctttac gcttcaggat cggctggatt taacccatga
    15841 gtatctgcac ctggcctttg atggttatcc caccgggctt gatgagaact atatcgaaac
    15901 gctgacccga caattattaa tggactaatg ctatgcgaaa gatttttctt ccgttgttac
    15961 tggtggcact ttcgcctgtc gctcacagtg aaggtgtgca ggaagtcgag attgacgcac
    16021 cgctctctgg ctggcatccg gcagagggcg aagatgccag tttttcacaa tcgatcaatt
    16081 acccggcgtc gtccgtcaac atggcagatg atcaaaatat ctcagcgcag atccgcggca
    16141 aaataaagaa ttatgctgcg gcgggtaaaa ttcagcaggg ccggctggtg gtcaacggtg
    16201 ccagtatgcc gcagagagtt gaatccgatg gttcatttgc acgcccttat attttcactg
    16261 aaggcagcaa cagcgtgcag gtcatcagcc cggatgggca aagccgacaa aaaatgcagt
    16321 tttactcaac gccgggtaca ggaacgattc gtgcacgttt acggctggtt ctctcgtggg
    16381 atacggacaa taccgatctc gatctccatg ttgtcacgcc cgatggcgaa cacgcctggt
    16441 acggtaacac cgtgctgaaa aacagtggtg cactggatat ggatgtcacg acggggtacg
    16501 gacccgagat tttcgccatg ccagcgccag ttcacggacg ctatcaggtg tatatcaact
    16561 actatggtgg gcgcagcgaa acggaattaa ccaccgccca actgacgctg atcaccgatg
    16621 aaggatcggt caatgagaaa caggaaacat ttattgtacc gatgcgtaat gctggcgagc
    16681 tgacgctggt gaaaagtttt gactggtgat ccatcccttc acacaacctg gctctctctt
    16741 ttttcagata agactgctgg gcgatctgcc gacgccagga tataaccatt aacaagcgtc
    16801 ggcagattgg tcatttctta tttttcgtac tgagaactgt ccatctactc cggctttcct
    16861 ggttaaggca atggtttcta agcgatacgc attattgcaa tggtgacaga acattgcggt
    16921 aacacgcttt taccgctacc ttaaccacac tccatcggtc acctgaggcg gagcttcgcc
    16981 cctttgaaat accttgcttc ttttcgtaca accctgaaag cctcgcgcta catgttcaga
    17041 gcattggcgt tagtgctctg gctgttgatt gctttttcat ccgtttttta catcgttaat
    17101 gcgttacatc agcgagaatc ggaaattcgt caggaattta atctgagttc cgatcaggct
    17161 cagcgcttta ttcaacgcac ctctgatgtg atgaaagagc tgaagtacat cgccgaaaat
    17221 cgcttatcgg cagaaaacgg tgtgctttcc ccgcgtggac gagaaacgca ggcggatgtg
    17281 cctgcgtttg aaccgctgtt tgccgactcc gattgttccg caatgagtaa cacctggcga
    17341 ggttctctgg agtcattggc gtggtttatg cgctactggc gcgataattt ttctgcggct
    17401 tacgatctaa accgggtatt tttaatcggc agcgataacc tctgcatggc caatttcggt
    17461 ctgcgtgata tgccagtgga acgcgatacc gcgttgaaag ctttgcatga acgcatcaat
    17521 aaatatcgaa atgcaccaca agatgatagc ggcagtaacc tctactggat cagcgaaggt
    17581 ccgcgccctg gcgtcgggta tttttacgcg ttgacgccag tttatctggc gaaccggttg
    17641 caggcgcttt tgggtgtcga gcagaccatc cggatggaga actttttctt accgggtacg
    17701 ttgccgatgg gggttaccat tcttgatgaa aatggtcata ccctgatttc gcttaccgga
    17761 ccagaaagta aaattaaggg cgatcctcgc tggatgcagg aacgctcctg gtttggctat
    17821 acggaagggt tccgggagct ggtgctgaag aaaaatctgc caccctcatc gctaagcatc
    17881 gtgtattcgg tgccggttga taaggtgctg gaacgcattc gcatgttgat ccttaacgca
    17941 attttgctga atgtgcttgc cggagctgca ttgtttactc tcgcacggat gtacgagcga
    18001 cgtattttca ttccggcgga aagcgacgcc ctgcgactgg aagaacatga gcagttcaat
    18061 cgcaagattg tcgcctccgc gccagtgggt atctgcattt tgcgtaccgc tgatggcgtc
    18121 aatattttaa gtaacgaact ggcgcatacc tatctcaata tgcttacgca tgaggaccgc
    18181 caacgactga cgcaaattat ctgtgggcag caggtcaatt ttgttgatgt cctgaccagc
    18241 aacaatacca atctgcaaat cagcttcgtc cattcgcgct atcgtaatga aaacgtggcc
    18301 atttgtgtgc tggtggatgt ttcttcgcgc gtgaagatgg aagagtcgtt gcaggagatg
    18361 gcacaagcag cggaacaggc gagccagtca aaatcgatgt tccttgccac cgtcagtcat
    18421 gagctgcgaa cgccgctgta tggcattatc ggtaacctgg atctgttgca aaccaaagag
    18481 ttaccgaaag gcgtcgatcg gctggtgacg gcaatgaaca actcttccag cctgttgttg
    18541 aaaattatca gcgatattct cgatttctcg aagattgaat cggaacagtt gaagatcgaa
    18601 ccgcgtgagt tttcaccgcg tgaagtgatg aaccacatca ccgccaacta tttaccgctg
    18661 gtggtacgca agcagttagg cttgtactgc tttattgaac cggatgtgcc agtggcctta
    18721 aatggcgacc cgatgcgttt acagcaggtc atctccaacc tgttgagtaa cgccataaaa
    18781 ttcaccgata ccggctgtat agttttgcat gtccgcgctg atggcgatta tctctctatc
    18841 cgtgttcgcg ataccggcgt ggggattccg gcgaaagaag tggtgcgctt gtttgatccc
    18901 ttcttccagg tcggaacggg cgtacagcga aacttccagg ggaccggtct gggtctggcg
    18961 atttgtgaaa aactgatcag catgatggac ggcgatatct cggtagattc agaaccggga
    19021 atgggcagcc agtttaccgt gcgtattccg ttgtacggcg ctcagtaccc gcagaaaaaa
    19081 ggcgtggaag ggttgagtgg taaacgctgc tggctggcgg tccgcaatgc gtcgctctgt
    19141 cagttcctgg aaaccagttt gcagcgcagc ggcatcgtcg ttacaacata cgaagggcag
    19201 gaaccgactc ccggagatgt gttaattact gacgaggtag tgagtaaaaa atggcagggt
    19261 agagcggtag tgaccttctg tcgtcgacat attggtattc cgctggagaa agcgccaggg
    19321 gagtgggtac acagtgtggc tgccccgcat gagctaccgg cattgttggc gcgtatttat
    19381 ttgatcgaga tggagagcga cgatcctgct aacgctctgc cgtcgacgga caaagcggtc
    19441 agcgataacg acgatatgat gattctggtc gtggatgatc atccgattaa ccgacgtctg
    19501 ctggcagatc agttgggatc gttgggctat caatgtaaaa ccgcgaatga tggcgtcgat
    19561 gcgcttaatg tacttagcaa gaatcatatt gatatcgtgc ttagcgacgt caacatgcca
    19621 aatatggatg gttaccgctt gacgcaacgc attcgtcagt tgggactgac gttgccggta
    19681 atcggagtaa ctgctaatgc gttggctgaa gagaagcagc ggtgtctgga gtccggtatg
    19741 gacagctgcc tgtcgaagcc ggtaacgctg gatgtgataa aacagacgct gacggtatat
    19801 gccgagaggg tcaggaaatc gcgggaatcg taggacggat aaggcgttta cgccgcatcc
    19861 ggcattcagt gcaaatgcca gataagacac taacgcgtct tatctggcct acaggtgatt
    19921 agtctttatc tgccggactt aaggtcacag aagagagata attcagcagg gcgatatcgt
    19981 tctcgacacc cagcttcatc atcgcagatt tcttctggct actgatggtt ttaatactgc
    20041 ggttcagctt tttagcgatc tcggtcacca ggaagccttc cgcaaacagg cgcagaactt
    20101 cactctcttt tggcgagaga cgcttgtcac cgtaaccacc agcactgatt ttttccaaca
    20161 ggcgagaaac gctttccggg gtaaatttct tccctttctg cagcgcggcg agagctttcg
    20221 gcagatcggt cggtgcacct tgtttcagca cgatcccttc gatatccaga tccaataccg
    20281 cactaagaat cgccgggttg ttgttcatgg tcagaacaat aatcgacagg cttgggaaat
    20341 ggcgcttgat gtacttgatt aaggtaatgc catcgccgta cttatcgcca ggcatggaga
    20401 gatcggtaat caacacatgc gcatccagtt tcggcaggtt gttgatcagt gctgtagagt
    20461 cttcaaattc gccgacaaca ttcacccact caatttgctc aagtgatttg cgaataccga
    20521 acaagactat cggatggtca tcggcaataa ttacgttcat attgttcatg taataggcta
    20581 ccttgctaca gcaagctctt gacataactg tcaatgtcgc tgatgtattt ttctattcct
    20641 ggaacatcct tctcacgaat cagatgttcc agcgtttcac ataactgctt gccgggtacc
    20701 agatttagca tggcaaatac gcctttaaga cgatgagccg tttgggctaa cgcagcaaag
    20761 tcactggttg ctgcttcagt atacagcctc ttaacatcat ccggtactgt gtctacaaag
    20821 agcgcataat agccgctggc atggagttgc gcattttcat ctccgcccag aggcgattct
    20881 gtcacctctt cctgcgccag ttgcacttca attaattgta agaccgcttc ctgcatagcg
    20941 ttgctcatat tgaagttgac gcacaattga ccaggcccaa tttcccgtac gccagactca
    21001 tcatcgctta aaagcaagca agaggcagta agattagacg gattatccgt taaaaagata
    21061 tcataatctt gactaattaa tctttcatcg ggtgtgatac aggttgcacc ccaattttct
    21121 aactggcgag tgacaatatt ccgaatttct gccgaagtaa catccaccat tacgcagaca
    21181 tcatccagta aacgctcttc ttcctcttca acttccgggt cagcggcgag cattttgata
    21241 tgcacagagt agcgtgtacc aagcccatcc cgcgttttga tgtttaaatg accgcccagt
    21301 ttacgtgcca gttgatcgct cagccagaat gccagcgggt ccgcctttcc ataacgatcg
    21361 ttttgggtct ggttgataaa cgggaagtgc aaattatcca tttcatgaat acttacgcct
    21421 tctccggtgt ccagaatgcg gaatgtcagg cggtcttcag aggactcatc ctgatcaacc
    21481 tcaagggtga tttttcccaa ttgcgttgag gtcacggcat attgcatcag tagcagcaaa
    21541 atacgtcgta aggcatcgcg atcgccgcgg cgcatatcgt gtgctttcag atgattgtta
    21601 atcagcagtt gcagaccttt acgcttgatg gcaggcaaca ctgaaggcac aacttcatca
    21661 attaaatcct gcacggagaa cagcaccgtc tcacttttcc agctatcgtc cgcaagcatg
    21721 ttcgctaact gtatttcatc gaccagccgc accagcacat ctgcctgatt cgccagttgt
    21781 ttgctttccg gggcgttgag tttagccgcg ctctccgcca gggactgtgc aggttctttc
    21841 agcgcatcgc caatgttttt cataaaggtc atccggccct gctggttttt ctcatacaga
    21901 cgctgcgcct gcttgagttt cttgtttacc agcacttcgc gatcctgatc gcgaataatg
    21961 aaaatttgtg tgcgcggcgc gacctggctg cggaacatgc ggatctcata cagctcgtta
    22021 ttgatcgtcg cctgaataat cccctgatgc tgttccgcca tggtggtgat gttttgcaga
    22081 ttcaaatgcg gcagcaaatg atcggcaatt ttgttactta tgacagtgcg gttcgattcc
    22141 tgatcgtaaa ccagcaggcc gagcggcagc agtgagacta tctcttcatt gattgcccgt
    22201 aaaatgcgca attcgttatt gaccgccgtg ttgggtagac tttctgtact gcgactggag
    22261 aaatggcgga atgtggtata gccaaataac gccagcgcca gcaaaccgat gttcagcagc
    22321 agtggcagca gaatgttttg caacgtatcc agcaataagg tgccataagg aacctgccag
    22381 accaggcgca tatcggtaga gttgagtgcc gaggagattt caatcttcgt actgttaaag
    22441 tggatactga cgctatccgt tccttctttc tcattatcat tgtctcccgt tgccgtcgcg
    22501 tctggctcaa ggcggaaact gtccagcggc atacccggtg ggatcaaatc attaatcggc
    22561 agatcaaaag ccacgaccgt tgccagatgt cccggctggt tgaacgtggt acgcaaggta
    22621 aagtaatgac cgttctgcca ggccaggcgg cgcatgttag aaaaactttc gcgttcatcg
    22681 agggcgttgg cctgttgcaa catctctgca cgacgtgaat caacaatgtc gctgacggtc
    22741 gattctttaa atccggaggt gagatctttg aggggcaggg ttgagatcag taccagactg
    22801 ttatcctgac cattcaggta atacatcgac cacggtacat tttctgcacc ccacaatgta
    22861 tccagataag tggacatccg ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag
    22921 attaacgctt ccgttttgcg gcgtggtttc tccagatagt agacatcctg tttcaggcgc
    22981 gtctcttgta aaccttcgcc ggaggagggg gaggtcgtcg cggcaatgtt gtcgtagatc
    23041 tgccaggtca cgtaacgcca ggtatcgacg cgcttttgga ttgcgtgggt aatgtcgaca
    23101 atctggtaac ttttatcttt cagccaggcg ttaacggcgc tttgtaccat tacccccatc
    23161 gtcaccagta acacaatgat caacagtaaa aagaagcggg taatgctccc cggtaggagt
    23221 gaaaagcggg tcgtggccgt tgtctctttc tgacgcattc gtgtttatga cctgttaaaa
    23281 cttcgcgaag tgatagggca gtataaaggg tacagtgtga aattccagac tcttacctga
    23341 ttgatgattc tctattctcc cgtgatattt atcatcctgc attgggggaa atagatgtac
    23401 aagcgccatt ttgctgtaca aataacctac aaaaagcgca acgaaattaa ttgcaaataa
    23461 acgaagattg ctggaaatta tgctgatgtt aattatttgt gaaatagtta acaagagtta
    23521 tagtttttct gtggtagcac agaataatga aagtgtgtaa agaagggtaa aaaaaaccgg
    23581 atgcgaggca tccggttgaa ataggggtaa acagacattc agaaatgaat gacggtaata
    23641 aataaagtta atgatgatag cgggagatat tctagttgcg agtgaaggtt ttgttttgac
    23701 attcagtgct gtcaaatact taagaataag ttattgattt taaccttgaa ttattattgc
    23761 ttgatgttag gtgcttattt cgccattccg caataatctt aaaaagttcc cttgcattta
    23821 cattttgaaa catctatagc gataaatgaa acatcttaaa agttttagta tcatattcgt
    23881 gttggattat tctgcatttt tggggagaat ggacttgccg actgattaat gagggttaat
    23941 cagtatgcag tggcataaaa aagcaaataa aggcatataa cagagggtta ataacatgaa
    24001 agttaaagta ctgtccctcc tggtcccagc tctgctggta gcaggcgcag caaacgctgc
    24061 tgaagtttac aacaaagacg gcaacaaatt agatctgtac ggtaaagtag acggcctgca
    24121 ctatttctct gacaacaagt cagaagacgg cgaccagacc tatgtacgtc ttggtttcaa
    24181 aggcgaaact caggttactg accagctgac cggttacggc cagtgggaat atcagatcca
    24241 gggcaatacc tctgaagaca acaaagaaaa ctcctggacc cgtgtggcat tcgcaggtct
    24301 gaaattccag gatgtgggtt ctttcgacta cggtcgtaac tacggcgttg tttacgacgt
    24361 aacttcctgg accgacgtac tgccagaatt cggtggcgac acctacggtt ctgacaactt
    24421 catgcagcag cgtggtaacg gcttcgcgac ctaccgtaac actgacttct tcggtctggt
    24481 tgacggcctg aactttgctg ttcagtacca gggtaaaaac ggtagcataa gcggcgaagg
    24541 catgaccaac aacggtcgtg gtgctctgcg tcagaacggc gacggcgttg gcggatctat
    24601 cacttatgat tacgaaggct tcggtatcgg tggtgcgatc tccagctcca aacgtactga
    24661 tgatcagaac agcccgctgt acatcggtaa cggcgaccgt gctgaaacct acaccggtgg
    24721 tctgaaatac gacgctaaca acatctacct ggctgctcag tacacccaga cctataacgc
    24781 aactcgcgta ggttccctgg gttgggcgaa caaagcacag aactttgaag ctgttgctca
    24841 gtaccagttc gacttcggtc tgcgtccgtc tgtagcatac ctgcagtcta aaggtaaaaa
    24901 cctgggtacc atcgctggtc gtaactacga cgacgaagat atcctgaaat atgttgatgt
    24961 tggtgctacc tactacttca acaaaaacat gtccacctac gttgactaca aaatcaacct
    25021 gctggacgac aaccagttca ctcgtgacgc tggcatcaac actgataaca tcgtagctct
    25081 gggtctggtt taccagttct aatctcgatt gatatcgaac aaagggcctg cgggcccttt
    25141 tttcattgtt ttcagcgtac aaactcattt ttttggtgta ctcttgcgac cgttcgcatg
    25201 aggataatca cgtatggaaa taagctttac ccgcgtggca ctgctggctg ccgcgctctt
    25261 ctttgttggt tgcgatcaaa aaccacaacc cgccaaaacc cacgctactg aagttaccgt
    25321 tcttgaaggc aaaaccatgg gtaccttctg gcgtgccagc atcccgggca ttgatgccaa
    25381 acgcagtgcc gaacttaaag aaaagattca gacccagctg gacgccgacg atcagctgct
    25441 ttcgacctat aaaaaagatt ccgcgctgat gcgctttaac gactcgcaaa gtttgtcgcc
    25501 gtggccggta agtgaagcga tggccgatat cgtcaccacc tcgctgcgca ttggcgcaag
    25561 gaccgatggt gcgatggata taaccgtcgg gccgctggtg aatctgtggg gctttggccc
    25621 ggaacaacag ccggttcaaa ttccgagcca ggaacagatc gatgcgatga aagccaaaac
    25681 cggcttacag cacctgacgg tcatcaatca gtcgcatcag caatatctgc aaaaagacct
    25741 gccggattta tatgtcgatc tctccaccgt cggtgaaggt tatgcggcgg atcacctggc
    25801 acgcttgatg gagcaggaag ggatttcccg ctatctggtg tcggtgggcg gcgcgctgaa
    25861 cagccgtggt atgaacggtg aaggccagcc gtggcgggta gcgattcaaa aaccaaccga
    25921 taaagaaaac gcggttcagg ctgtggtgga tatcaacggt catgggatta gcacctctgg
    25981 cagctaccgt aactattacg aactggacgg caaacgtctt tcccatgtta tcgatccgca
    26041 aaccgggcgt cccatcgaac acaatctggt atccgtgacg gtgattgctc cgacggcgct
    26101 ggaagccgat gcctgggata ctggcttgat ggtactcggg ccggagaaag ccaaagaggt
    26161 tgttcgccgg gaagggctgg cggtttatat gatcaccaaa gaaggcgata gctttaaaac
    26221 ctggatgtca ccacagttta aaagcttcct tgtcagcgaa aaaaattaaa gcgcaagatt
    26281 gttggttttt gcgtgatggt gaccgggcag actaaaggct atccttaacc agggagctga
    26341 ttatgaaaaa agccacatgc ttaactgacg atcaacgctg gcaatctgtc ttagcccgcg
    26401 acccgaatgc tgacggcgaa ttcgttttcg ccgtgcgtac cacaggcatc ttttgccgtc
    26461 cgtcttgccg cgccagacat gctttgcggg aaaacgtctc cttctacgca aatgccagcg
    26521 aggcactcgc cgctggcttt cgcccctgca aacgttgtca gccagaaaaa gccaatgccc
    26581 agcaacatcg gttggataaa atcacccatg cgtgtcgact gctggaacag gaaacgcctg
    26641 taacgctgga agccttagcc gaccaggtgg cgatgagtcc atttcatcta catcggttgt
    26701 ttaaagcgac taccggaatg acgcctaaag cctggcaaca ggcctggcgc gctcgccgtt
    26761 tgcgcgaatc gctggcgaaa ggggagagcg tgacgacgtc tattcttaac gccggattcc
    26821 ccgacagcag cagttactat cgcaaagctg acgaaacgct gggcatgacg gctaaacaat
    26881 tccgtcacgg tggcgaaaat ctggcggtgc gttacgcgct ggctgattgt gagctgggtc
    26941 gttgcctggt ggcagaaagc gagcggggga tttgcgcgat attgctgggc gatgatgacg
    27001 cgacactaat cagcgagttg cagcagatgt ttcccgctgc cgacaacgcg cctgccgatc
    27061 tgatgtttca gcaacatgtg cgtgaagtga tcgccagcct caatcaacgc gatacgccgc
    27121 tgacgttacc gctggacatt cgcggcactg cttttcagca acaagtctgg caggcactgc
    27181 gcacgatacc ttgcggtgaa accgtcagtt atcagcaact ggctaacgcc atcggcaaac
    27241 cgaaagcggt acgggccgtt gccagcgcct gtgccgccaa caagctggct atcataatac
    27301 cctgtcatcg ggtggtccgt ggtgatggca cactttccgg ttaccgctgg ggcgtgtcgc
    27361 gtaaagcgca actgctgcgc cgcgaagctg aaaatgagga gaggtaatgt tggatctgtt
    27421 tgccgatgct gaaccgtggc aagagccact ggcggctgga gcggtaattt tgcaccgttt
    27481 tgcttttaac gctgcggagc aactaatccg cgatattaat gacgttgcca gccagtcgcc
    27541 gtttcgccag atggtcaccc ccgggggata taccatgtcg gtggcgatga ccaactgtgg
    27601 gcatctgggc tggacgagcc atcggcaagg ttatctctat tcgcccattg atccgcaaac
    27661 aaataaaccg tggcccgcca tgccacagag ttttcataat ttatgtcaac gtgcggctac
    27721 ggcggcgggc tatccagatt tccagccaga tgcttgtctt atcaaccgct acgctcctgg
    27781 cgcgaaactg tcgctgcatc aggataaaga cgaaccggat ctgcgcgcgc cgattgtttc
    27841 tgtttctctg ggcttacccg cgatttttca atttggcggc ctgaaacgaa atgatccgct
    27901 aaaaagattg ttgttggaac atggcgatgt ggtgatatgg ggcggtgaat cgcggctgtt
    27961 ttatcacggt attcaaccgt tgaaagcggg gtttcatcca ctcaccactg actgccgcta
    28021 caacctgaca ttccgtcagg caggtaaaaa agaataaaaa taagaattat tattgctgtg
    28081 cgcgcgaaga tgtttaaact gcgggctgta attcattgtc cgggttttct gcatggaact
    28141 tcttgtactt gtctggcggc agtatcgctg gccatttatc agtgtgatgg cgctaagcct
    28201 cgccagtgcg gcattaggca ttggcttaat tgcttttatc aatcagcgcc ttatcgaaac
    28261 ggcggatacc agtctgctgg tgttgccgga gtttctggga ttattgctgc tgttgatggc
    28321 agtcactctc ggatcgcaac tggcgctcac cactttgggg catcacttcg tttaccgact
    28381 gcgtagtgaa tttatcaagc ggattctgga tacctacgtc gagcgcattg aacaactcgg
    28441 tagcgcctcg ttgctagcgg gattaaccag cgatgtgcgc aatatcacca ttgcttttgt
    28501 gcgtctgccg gaactggtgc aggggatcat tctcactatc ggttccgcgg cgtatctgtg
    28561 gatgctgtcg ggcaaaatgt tgctggtaac ggctatctgg atggcgatca ccatctgggg
    28621 cggttttgtg ctggtggcgc gggtgtacaa acatatggcg accctgcgtg aaaccgaaga
    28681 caagctgtac acggattttc aaaccgtact cgaagggcgc aaagagttga ctctgaaccg
    28741 ggaacgcgcc gagtatgtgt ttaacaacct ctacatcccc gatgcgcaag agtatcgcca
    28801 tcatattatt cgcgcagaca ccttccatct tagcgccgtg aactggtcaa acatcatgat
    28861 gctgggcgct atcggcctgg tgttctggat ggcgaacagt ctcggttggg ctgataccaa
    28921 cgttgctgcg acctattcgt tgacgctgtt attcctgcgt acgccgctgc tttcggcggt
    28981 tggcgcattg ccgacgctgc tgacggcgca ggtggcgttt aacaagctga acaaattcgc
    29041 gctcgcgcct ttcaaagctg agtttccgcg cccgcaggct tttcccaact ggcaaacgct
    29101 ggagctgcgt aacgtgacgt ttagctatca ggataacgcg ttttccgttg ggccgattaa
    29161 cctcaccatt aaacgtggcg agctgctgtt tctgattggt ggcaacggta gtggaaaatc
    29221 gacgctggcg atgttgctga cgggcttgta ccagccacaa agcggcgaaa tcttgctgga
    29281 tggcaaacct gtcagtggcg aacaaccgga agattatcgc aaactgtttt cggcagtgtt
    29341 taccgatgtc tggctgtttg atcaactgct ggggccggag ggtaaacccg ctaacccgca
    29401 actggttgag aagtggctgg cgcagctgaa aatggctcat aagcttgagt taagcaacgg
    29461 gcgtattgtt aacctgaagt tatcaaaagg gcagaaaaaa cgcgtggcgc tgttgctggc
    29521 gctggcagaa gaacgcgata ttatcctgct ggatgaatgg gcggcggatc aggatccaca
    29581 cttccgtcgt gagttttatc aggtgttgct gccgctgatg caggagatgg gtaaaactat
    29641 tttcgctatc agtcatgatg atcattactt tatccacgcc gaccgcctgc tggaaatgcg
    29701 caatgggcaa cttagcgagc tgacgggcga agagcgcgat gccgcttcgc gtgatgccgt
    29761 tgcccggacg gcataatatt acgacgctga cctggcggac tgctgccgtc aggtcaatat
    29821 gctaaaaatc ccttcatttc taataaccct ataattaatc aacgaaatta taatgtttct
    29881 aaaattagaa tataatttat aaacattatt taaatgttgt tacttaagtg ttaccgttga
    29941 tgccgcgcaa atctcacctg caataaagcg actaaaagta aggcattaac aagatgaaaa
    30001 aagtgactgc catgctcttc tcgatggccg tggggcttaa tgccgtttcg atggcggcaa
    30061 aagcgaaagc gtccgaggag caggaaactg atgtactgtt gattggcggc ggcattatga
    30121 gcgccacgtt ggggacctat ttacgcgagc tggagcctga atggtcgatg accatggtgg
    30181 agcgcctgga gggtgtcgcg caggagagtt cgaacggctg gaataacgcc ggaaccgggc
    30241 attctgcact gatggaactg aactacaccc cgcaaaacgc cgatggcagc atcagtattg
    30301 aaaaagcagt cgccattaac gaagcatttc agatttcccg ccagttctgg gcgcaccagg
    30361 ttgagcgcgg cgtgctgcgt actccgcgtt catttatcaa taccgttccg catatgagct
    30421 ttgtctgggg cgaggataac gttaatttcc tgcgcgcccg ttacgccgcg ttgcaacaaa
    30481 gctcgctgtt tcgcggtatg cgttactctg aagatcacgc gcagatcaaa gagtgggcac
    30541 cgttagtgat ggaagggcgc gatccgcaac agaaagtggc agccacgcgt acggaaattg
    30601 gtaccgatgt gaactacggc gagatcaccc gccagttaat tgcttccttg cagaagaaat
    30661 ctaacttctc gctgcaactc agcagcgaag tccgcgccct aaagcgtaat gacgataaca
    30721 cctggaccgt taccgttgcc gatctgaaaa atggcactgc acagaacatt cgtgccaaat
    30781 ttgtctttat cggcgcgggc ggtgcggcgc tgaagctgtt acaggaatcg gggattccgg
    30841 aagcgaaaga ctacgccggt ttcccggtgg gcggacagtt ccttgtttcg gaaaacccgg
    30901 acgtggttaa tcaccatctg gcgaaggttt acggtaaagc atccgttggc gcaccaccga
    30961 tgtcggttcc gcatatcgat acccgcgttc tggacggtaa acgcgtagtg ctgtttgggc
    31021 catttgccac cttctcaacc aaattcctca aaaacggttc attgtgggat ctgatgagtt
    31081 ccaccaccac ctctaatgtg atgccgatga tgcacgtcgg gctggataat ttcgatctgg
    31141 tgaaatattt ggtgagtcag gtgatgttga gtgaagagga tcgttttgaa gcgttgaaag
    31201 agtactatcc gcaggcgaaa aaagaggact ggcgtttgtg gcaagcgggg cagcgcgtgc
    31261 agattatcaa gcgtgatgcc gagaaaggtg gcgtactgcg tttgggtact gaagtggtca
    31321 gtgaccagca agggaccatt gccgcgctgc tgggggcatc gccaggggcg tcaactgccg
    31381 cgccgattat gttggatctg ctggaaaaag tatttggcga tcgcgtttcc agcccgcaat
    31441 ggcaggctac gttgaaagcg atcgttccgt cttatggacg caagctgaac ggtgatgtag
    31501 cggcaacaga acgcgagttg cagtacacca gcgaagtgct ggggctgaaa tacgacaagc
    31561 cgcaggcagc agatagtacg ccaaaaccgc agttgaaacc gcaacccgtt caaaaagaag
    31621 tggcggatat tgcgttgtaa tgatgtgcca catccggcat ggtatgccgg atgtggcgta
    31681 tactgataag acgcgccagc gtcgcatcag gcaaccggct tgggcgttag atgtcggatg
    31741 cggcgtgacc gccttatccg acctacgggg tgacgcactt cacagcagtt tagcgaacta
    31801 ccgcgttgtc aattttctct tccgccttcc agacgcggta cttcacatct acattgtctg
    31861 gcgtatacac cacgatcggc agcttgctgt tgtaacgcag cattccagca tcgcccagat
    31921 acgcggtgac aaatttcttc tctttcttgc catccgggca ggccatcatc gtggaaaccg
    31981 gcgagctaac tttatcaaag acataataat catagcccca gccttccagc gttttgtttt
    32041 ccagcttccc gccgagacga tgcaaattgc aatcgacttc cagcgtctga ccgattaaca
    32101 gttctacttt cagggtagat tcatcttctt gcggggttaa ctgaatcacc tgacgcttca
    32161 tccctttttc agcttgtgga taaggcgcga ttttttccag tggctggacg ctttctgccg
    32221 cccaggcgga agtggtggcg aaagcggcaa acaatactgc aggtagaatg gtcttcattt
    32281 ttttgctttc cttcttcatt tataggtttt caccgatgat gtcgctgaag gttaacatat
    32341 tcatcagatt tattaatttg ctttatgaat aacttaaaga acattcagac aattttacct
    32401 gctcttttag tgttaaattc tgatgagggg gcaaacaagc agaaatatct ctggtgaatt
    32461 aatcgctata taaatatgtt tataaccatt tgaaatgtga gcaaaagccc gtttttgcca
    32521 cgcttcccgc cactcttttg atcctgctac aggttttacc ccgatcgggg tatgcatctt
    32581 tgacacatcc tttaatatct tagcggctat aaaaatggct tattaattat gcggtttatt
    32641 tggtcgctct caattttcag agcgcgttaa tgatggaagg tcaatgtgaa gattgatgca
    32701 tcccgtcggg gcatactcac tggtcgctgg cgcaaagcca gtaacggtat ccgtccgccc
    32761 tggtccggtg atgaatctca ttttctgacc cattgcaccc gctgcgacgc ctgtattaat
    32821 gcttgtgaaa acaacattct gcaacgcggc acgggtggct atccgagcgt taatttcaaa
    32881 aataacgagt gcagcttctg ttacgcctgc gcgcaggctt gccccgaatc gctattttct
    32941 ccgcgccaca ccagggcctg ggatttgcag ttcaccatcg gggacgcgtg cctggcgtat
    33001 cagtcagttg aatgccgccg ctgtcaggat agctgtgagc caatggcaat tatctttcgc
    33061 ccgacgctct ccgggatcta ccagccgcaa cttaatagcc aactttgcaa cggatgtgga
    33121 gcatgtgcag ccagctgtcc ggtatcagcc ataactgcgg agtatctcca tgcacactaa
    33181 ctggcaagtt tgcagcctgg tcgtgcaggc caaaagcgaa cgaatttcag acatcagcac
    33241 ccaactgaac gcctttcccg gctgtgaagt tgctgtcagc gacgcgccga gcggtcagtt
    33301 gattgtggtg gtggaagcag aagacagcga aacgctgatc caaaccattg agtcagtacg
    33361 caacgtagag ggcgtgctgg cggtgtcgct ggtttatcac cagcaggaag agcaaggtga
    33421 ggaaacacca tgaaactcag tcgtcgtagc tttatgaaag ctaacgccgt tgcggccgct
    33481 gcggcggctg ccggtctcag cgtgccgggc gttgcccgcg ccgttgttgg tcagcaggaa
    33541 gccattaaat gggataaagc gccgtgccgt ttctgcggta ctggttgcgg cgttctggtc
    33601 ggaacgcagc aggggcgtgt ggtggcctgt cagggcgacc cggacgcacc ggttaaccgt
    33661 ggcctgaact gcattaaggg ctatttcctg cccaagatca tgtacggtaa agaccgtttg
    33721 acgcagccgc tgctgcgtat gaaaaacggt aaatatgaca aagaaggcga atttacccca
    33781 atcacctggg atcaggcctt cgatgtgatg gaagagaaat tcaaaaccgc cctgaaagaa
    33841 aaagggccgg aatcgatcgg tatgttcggt tctggtcagt ggactatctg ggaaggttat
    33901 gccgcgtcca agctgtttaa agcgggcttc cgttcgaaca acatcgaccc gaacgcgcgt
    33961 cactgtatgg cgtcggcagt agttggcttt atgcgtacct ttggtatgga tgagccgatg
    34021 ggctgctatg acgacatcga gcaggctgac gcgtttgtgc tgtggggcgc aaacatggcg
    34081 gagatgcacc cgatcctctg gtcgcgcatc actaaccgtc gtctctctaa tcagaacgtc
    34141 accgtggcgg tgctttctac ctaccagcat cgtagcttcg agctggcgga taacggcatc
    34201 atctttacgc cacagtctga cctggtgatc ctgaactaca tcgccaacta tatcattcaa
    34261 aacaatgcga taaatcagga cttcttcagc aaacacgtta acctgcgcaa aggggcgacg
    34321 gacattggct acggtttacg tccgacccat ccgctggaaa aagcagcgaa gaatccaggt
    34381 tctgacgcct ccgaaccgat gagctttgaa gattacaaag ccttcgttgc cgagtatacg
    34441 ctggaaaaaa ctgccgaaat gaccggcgtg ccgaaagacc agttagaaca actggcgcag
    34501 ctgtatgccg atccgaacaa gaaagtcatc tcctactgga cgatgggctt caaccagcat
    34561 actcgtggcg tgtgggctaa caacctggtc tacaacctgc acctgctgac cggcaaaatt
    34621 tcccagccgg gttgcggtcc gttctccctg accgggcaac cttctgcgtg tggtactgcg
    34681 cgtgaagtgg gcacctttgc tcaccgtctg cctgcggaca tggtggtgac caacgagaaa
    34741 caccgtgata tctgcgagaa gaagtggaat atcccgagcg gcaccattcc ggcgaaaatc
    34801 ggtctgcacg cggtggcaca agaccgtgcg ctgaaagacg gcaaactgaa tgtttactgg
    34861 accatgtgta ccaacaacat gcaggccggg ccgaacatca atgaagagcg tatgccgggc
    34921 tggcgcgatc cgcgcaactt catcatcgtc tccgatccgt atccgacagt cagtgcgctg
    34981 gccgccgact tgatcctgcc gaccgcaatg tgggtagaga aagagggcgc ttacggtaac
    35041 gccgaacgcc gtactcagtt ctggcgtcaa caggtacagg caccgggcga agcgaaatcg
    35101 gatctctggc agttagtcca gttctcccgc cgcttcaaaa ctgaagaagt atggccggaa
    35161 gatctgctgg cgaagaaacc ggaactgcgt ggcaaaacgc tgtacgaagt gctgtatgcc
    35221 acaccggaag tgagcaaatt cccggtatcc gaactggcgg aagatcagct gaacgatgaa
    35281 tcccgcgagc tgggcttcta tctgcaaaaa gggctgttcg aagagtacgc atggtttggt
    35341 cgcggtcacg gtcacgatct ggcaccgttc gatgactacc acaaagcgcg cggtctgcgc
    35401 tggccggtgg tgaacggtaa agaaacgcag tggcgttaca gcgaaggtaa cgacccgtac
    35461 gtgaaagcgg gcgaaggcta taagttctac ggtaaaccgg atggcaaagc ggtgatcttc
    35521 gcactgccat tcgaaccggc ggcggaagca ccggatgaag agtacgacct gtggctctct
    35581 accggacgcg ttctggagca ctggcacacc ggcagtatga ctcgccgtgt accagaactg
    35641 caccgcgcct tcccggaagc ggttctgttt attcacccgc tggatgcgaa agcgcgcgat
    35701 ctgcgtcgtg gcgacaaagt gaaagtggtt tctcgccgtg gcgaagtgat ctcgattgtc
    35761 gaaacgcgcg gtcgtaaccg tccgccacag ggcctggtgt acatgccgtt cttcgacgcc
    35821 gcacagctgg ttaacaaact gacgctggat gcgaccgatc cgctctcgaa agagacggac
    35881 ttcaagaagt gcgcggtcaa actggagaag gtgtaagcca ttatgtcccg gtcagcgaaa
    35941 cctcaaaatg gtcgccgccg ctttctgcgc gatgtcgttc gcacagcagg cgggctggct
    36001 gccgtgggtg tggcgctggg gttacaacag caaaccgcac gcgcatctgg cgtgcggttg
    36061 cgcccgcccg gagccataaa cgagaacgcc tttgccagtg cctgtgtgcg ttgcggtcag
    36121 tgtgttcagg cttgccctta cgacacctta aaactggcga cgctggcctc tggtctgtcg
    36181 gcgggcacgc catattttgt cgcacgggat attccttgcg aaatgtgtga ggacattccg
    36241 tgcgccaaag tgtgcccaag cggtgcgctg gatcgtgaga ttgaatcgat cgacgacgcg
    36301 cggatggggc tggcggtact ggtggaccag gaaaactgtc tcaactttca ggggctgcgc
    36361 tgcgatgttt gttatcgcga atgcccgaaa attgatgagg ccatcaccct ggagctggag
    36421 cgcaacacgc gtaccggtaa gcacgcccgc tttctgccga cggttcacag cgacgcctgt
    36481 accggttgcg gtaagtgcga aaaagtatgc gtgctggaac aaccggcaat caaggtgtta
    36541 ccgctgtcac tggcgaaagg ggagttaggt caccattacc gcttcggttg gctggagggg
    36601 aacaatggca aatcgtaaac gtgacgccgg gcgcgaggcg ctggagaaaa aaggctggtg
    36661 gctcagtcac cgttggctgg tgttacgtcg cctttgtcag ttcttcgtgc tggggatgtt
    36721 tttgagcggt ccatggtttg gtgtgtggat cttgcacggc aactacagca gtagcctgtt
    36781 attcgacacc gttccgctga ccgatccgct gatgacgctg caaagtcttg ccagtggtca
    36841 tctgcctgcc acggtggcgt tgaccggtgc ggtgattatc accgtgctct acgccctggc
    36901 gggaaagcga ttattttgca gctgggtctg cccgctgaac ccgattaccg acttagcgaa
    36961 ctggttacgc aggcgctttg acctcaatca gtctgcgacg atccctcgcc acattcgcta
    37021 cgtgctgttg gtggtcattc tggtgggatc agccttaact ggcacgctca tttgggaatg
    37081 gatcaacccc gtttctctga tggggcgtag cctggtaatg gggttcggca gcggcgcgct
    37141 gcttattctc gcgctgtttt tatttgatct actggtcgtt gaacacggct ggtgcgggca
    37201 catttgtccg gttggcgcgc tgtatggcgt gctgggtagc aaaggtgtga ttaccgttgc
    37261 ggccagcgat cggcagaaat gtaaccgctg tatggattgt tttcatgttt gcccggaacc
    37321 gcatgtgcta cgtgccccgg tgctggatga acaaagcccg gtgcaggtca ccagccgcga
    37381 ttgcatgacg tgcggtcgct gcgtggatgt ctgttctgag gatgtattta caataactac
    37441 acgatggagt tcgggagcga aatcatgaaa agccatgacc tgaagaaagc gctgtgtcaa
    37501 tggacggcga tgctggccct ggtggtaagc ggcgcggttt gggcggctaa tggcgtcgat
    37561 tttagccagt cgccggaagt atccgggaca caggaagggg ccattcggat gccaaaagag
    37621 caggatcgga tgccgctgaa ctatgtgaat cagccgccaa tgatcccgca tagcgttgaa
    37681 ggttatcagg taacgaccaa taccaaccgc tgcttgcagt gccacggtgt cgaaagctat
    37741 cgcaccactg gcgcgccgcg tatcagtcct actcacttta tggacagcga cggcaaagtg
    37801 ggcgcggaag tggctccgcg tcgttatttc tgtctgcaat gtcacgtacc gcaggccgat
    37861 accgcgccaa tcgtggggaa tacctttacc ccatcaaaag gttacgggaa ataagaggtt
    37921 attatgggaa attctcaccg taagcctggg ctgattaagc gcctgtggaa atggtggcgt
    37981 acccccagcc gtctggcgct ggggacgctg ctgttgatcg gttttgtcgg cggcatcgtc
    38041 ttctggggcg gctttaacac tgggatggaa aaagccaata ccgaagagtt ctgcattagc
    38101 tgccacgaaa tgcgtaacac ggtgtatcag gaatacatgg attccgtgca ctacaacaac
    38161 cgtagcggcg tccgtgcgac ctgtccggat tgtcacgttc cgcacgagtt tgtgccgaag
    38221 atgatacgca agctcaaagc aagtaaagag ctgtatggta aaatttttgg cgttattgac
    38281 acgccgcaga aatttgaagc tcatcgtctg acgatggcac agaatgagtg gcggcgcatg
    38341 aaggacaata actcgcagga gtgccgtaac tgtcacaact tcgagtatat ggatacaacc
    38401 gcccagaaat cggttgccgc gaagatgcat gaccaggcgg tgaaagatgg gcaaacctgt
    38461 attgattgcc ataaagggat agcgcacaag ctgcccgata tgcgtgaagt cgagccaggt
    38521 ttttaacagg gttattgcgt gggtatgctt gaagccagag agttactttg tgagcgggat
    38581 gaacgaacct tatttagtgg cttgtcattt acgctgaact caggagagtg ggtacaaatc
    38641 accggtagca acggcgcggg gaagacaacg cttctccgtt tgctgacggg gttgtctcgc
    38701 cctgacgcag gcgaggttct ctggcaaggg cagcccttgc atcaggtacg cgacagctac
    38761 catcaaaacc tgttatggat aggccatcag ccggggatca aaacccggct gacggcgtta
    38821 gaaaatctgc acttttatca tcgcgatggc gataccgcac aatgtctgga agccctggcg
    38881 caggccgggc ttgccggatt cgaagatatt cctgtaaatc agctctcggc cgggcaacaa
    38941 cgccgcgtcg ctttagcgcg tctgtggctg acccgtgcca cgttatggat cctcgacgag
    39001 ccttttaccg cgattgacgt taacggcgtc gatcgtctga cccagcgtat ggcgcagcat
    39061 acggagcagg gggggattgt gattctgact acccaccagc cgctcaacgt tgctgaaagt
    39121 aaaattcgcc gcatttcact gacgcaaacg agggccgcat gatgttctgg cgcattttcc
    39181 gtcttgagct gcgtgtagcg tttcgccata gcgccgaaat cgccaacccg ctgtggttct
    39241 tcctgattgt aattaccctt tttccgctca gtatcggtcc ggagccgcaa ctgctggcgc
    39301 gtattgcacc gggcattatc tgggttgctg cgctgctttc atccttgctg gcgctggaac
    39361 gactgttccg tgacgatttg caggacggca gtcttgaaca attgatgttg ttgccgttac
    39421 ccttgcccgc cgttgtgctg gcgaaggtga tggcgcactg gatggtaacc ggtctgccgt
    39481 tactcatcct ttcgccactg gtagcaatgc tactgggaat ggatgtgtat ggctggcaag
    39541 tgatggcgct gacgctgctg ctgggaacgc ctacgcttgg ctttctcggt gcaccgggcg
    39601 tggcgctgac agtgggactt aagcgcggtg gtgtgctgct cagcatactg gtgttaccgc
    39661 tgactatccc attactcatc ttcgccaccg ccgcgatgga cgcggcttcc atgcatttgc
    39721 ccgttgacgg gtatctggca attttaggcg cgttgctggc aggcaccgcg acattaagtc
    39781 cttttgcgac ggcggcagcg ttacgaatca gcattcaata acgctggatt actgattttt
    39841 gttcgagtct ggtatcgaaa ctatgtggaa aacactgcat caactggcga tcccaccacg
    39901 gctgtatcaa atctgtggct ggtttatacc gtggctggca attgccagtg tggtcgtgct
    39961 taccgtcggc tggatctggg gattcggctt tgctccggct gattatcagc agggaaatag
    40021 ctaccgcatt atctacctgc atgtgcctgc ggcgatctgg tcgatgggca tttatgcatc
    40081 aatggcagtg gcagcgttta ttggccttgt ctggcagatg aaaatggcca acctggcggt
    40141 ggcggcgatg gcccccattg gtgccgtgtt tacctttatt gccctggtta ccggctctgc
    40201 atggggaaaa ccgatgtggg gcacctggtg ggtatgggat gcacgtctga cttctgaact
    40261 ggtgctgctg tttttgtatg tgggtgtgat tgccctgtgg cacgccttcg acgaccgccg
    40321 tctggcgggc cgtgcggcag gtatcctggt gctgattggc gtggtgaatc tgccgattat
    40381 tcattactcc gtggagtggt ggaacaccct gcatcaggga tcaacgcgga tgcagcaaag
    40441 tatcgatccg gcgatgcgtt cgccgctgcg ctggtcgatt tttggcttcc tgctcctgtc
    40501 tgccacgctg acgctgatgc ggatgcgtaa tttgattttg ctgatggaaa aacgccgtcc
    40561 gtgggtgagt gaactgatac tgaaaagagg ccgtaaatga cccctgcatt tgcttcctgg
    40621 aatgaatttt tcgcaatggg cggttacgcc ttttttgtct ggctggcggt ggtgatgacc
    40681 gttattccgc tggttgtttt ggtcgtgtac tcggtgatgc aacatcgcgc aattctgcgt
    40741 ggtgtggcgc aacagcgggc gcgtgaggcg cgtttacgtg ctgcgcaaca gcaggaggct
    40801 gcatgaatat tcgccgtaaa aaccgcttgt ggattgcctg tgccgtgttg gcagggctgg
    40861 cgctgactat cggtctggtg ctatatgcgc tgcgctcgaa tatcgatctc ttttatacgc
    40921 cgggggaaat tctctacggc aagcgtgaaa ctcagcaaat gccggaagtc ggtcagcgtc
    40981 tgcgcgttgg cgggatggtg atgccgggta gtgtgcagcg cgatcccaat tcgctgaaag
    41041 tgaccttcac tatttacgat gctgaaggct cagtggatgt ctcttacgaa ggcattttgc
    41101 cggatctgtt ccgtgaaggg cagggcgttg tggtgcaggg cgagctggaa aaaggcaatc
    41161 atatcctcgc gaaagaagtg ctggcgaaac acgacgaaaa ctacacgccg ccagaagttg
    41221 agaaagcgat ggaagctaac caccgtcgcc cggcgagtgt ttataaggac ccagcatcat
    41281 gatgccggaa attggtaacg ggctgctgtg tctggcgctc ggaattgcgc tgctgctgtc
    41341 cgtgtatccg ctatggggcg tggcgcgcgg agatgcgcgc atgatggcgt cttcccgctt
    41401 gtttgcctgg ctgctgttta tgtctgtggc tggcgcattt ctggtgctgg tcaatgcttt
    41461 cgtggtcaac gactttaccg tcacctatgt agccagcaac tctaataccc agcttccggt
    41521 gtggtatcgc gtggcggcta cctggggcgc gcatgaaggt tcgcttctgc tgtgggtgct
    41581 gctgatgagc ggctggacct ttgcggtggc gatttttagt cagcgtattc cgctggatat
    41641 tgtggcccgt gtactggcga taatggggat ggtcagcgtc ggctttttgc tgttcattct
    41701 ctttacctct aacccgttct cacgcacgct gccgaacttc ccgattgaag ggcgcgatct
    41761 taacccgctg ttgcaggatc cggggctgat cttccatccg cctctgctct atatggggta
    41821 cgtggggttc tcggtggcgt ttgcttttgc cattgcttct ttgttgagcg ggcgtctgga
    41881 cagcacttat gcgcgtttta ctcgtccgtg gacgctggca gcgtggatct tcctgacgct
    41941 cggcatcgtg ctcggttccg catgggccta ttacgaactc ggctggggtg gctggtggtt
    42001 ctgggatccg gtagaaaacg cctcgtttat gccgtggctg gtggggactg cgctgatgca
    42061 ctcactggcg gtcactgaac aacgcgccag cttcaaagcg tggacattac tgctggcaat
    42121 cagtgccttc tcgttgtgtc tgttggggac tttcctcgtg cgttccggcg tgctggtatc
    42181 ggtacacgcg tttgcgtctg atccggcgcg cggtatgttt atcctcgcct ttatggtgct
    42241 ggtgattggc ggttcgctgc tgctgtttgc cgcgcgtgga cacaaagttc gctcacgcgt
    42301 aaacaatgcg ctgtggtcgc gggaatcttt gctgttagcg aacaacgttt tgctggtcgc
    42361 cgcgatgctg gtggtgttgc tggggacgct gctgccgctg gtgcacaagc aacttggact
    42421 gggcagtatt tcgattggcg aaccgttctt caacaccatg tttacctggc tgatggtgcc
    42481 gtttgcgcta ctggttggtg tcggtcctct ggtgcgctgg gggcgcgatc gcccacgtaa
    42541 aatccgcaat ttattgatta tcgcctttat ctctacgctg gtgctgtctc tgctgttgcc
    42601 gtggctgttc gaaagcaaag ttgtggcgat gacggtgctc ggcctggcaa tggcctgctg
    42661 gattgcggtg ctggcaactg cggaagctgc gctgcgtatt ttacgcggca cgaaaaccac
    42721 cttcagttat tgggggatgg tggcggctca cctggggctg gcagtgacaa ttgttggcat
    42781 tgcctttagc cagaattata gcgttgagcg tgatgtgcgc atgaagtccg gcgatagcgt
    42841 cgatattcat gaatatcgct tcaccttccg tgatgtcaaa gaggtgactg gcccgaactg
    42901 gcgcggcggt gtggcgacta ttggcgtaac gcgagagggt aaaccggaaa cggtgctgta
    42961 tgcggaaaaa cgttattaca acactgccgg gtcgatgatg accgaagcgg caattgacgg
    43021 cggcatcacg cgtgacctgt acgccgcgct cggtgaagag ctggaaaacg gcgcgtgggc
    43081 cgtgcgtctt tactacaaac catttgttcg ctggatttgg gcgggcgggc tgatgatggc
    43141 gttgggcgga ctactgtgtc tgtttgatcc tcgctatcgt aagcgcgtga gtccgcaaaa
    43201 aactgcgccg gaggccgtat gaagcgcaaa gtattgttaa ttccgttgat tatcttcctg
    43261 gcgattgccg cggcgctgct gtggcagctg gcgcgtaatg ccgaagggga tgatccgacc
    43321 aatctggaat cggcgctcat tggcaagcct gtgccgaagt ttcgtctcga atcactggac
    43381 aatccggggc agttttatca ggcggatgtg ctgactcagg gcaaaccagt actgcttaac
    43441 gtctgggcga cctggtgtcc gacctgccgt gcggaacatc aatatctgaa tcagctttca
    43501 gcgcagggca ttcgcgtggt cggcatgaac tataaagacg atcgccagaa ggcaatcagc
    43561 tggctgaaag agctgggcaa tccttacgcg ctaagcctgt ttgatggcga cggcatgtta
    43621 gggctggatc tcggtgtcta tggcgcgcca gaaaccttcc ttattgacgg caacggtatc
    43681 attcgctatc gccatgcggg cgatctcaat cctcgcgtct gggaagaaga gatcaagccg
    43741 ctgtgggaga aatacagtaa ggaggccgca caatgaggtt tttattgggc gtgctgatgc
    43801 tgatgatctc cggctcagca ctggcgacca tcgacgtgtt gcagtttaaa gatgaagcac
    43861 aggaacaaca gttccgtcag ctcactgaag aactgcgctg cccgaaatgc cagaacaaca
    43921 gcattgccga ttccaactcg atgattgcca ccgacctgcg tcagaaagtg tatgaactga
    43981 tgcaggaagg taaaagtaaa aaagagattg tcgattatat ggtggcgcgt tacggcaact
    44041 tcgtcactta cgatccgccg ttaacgccgc tgaccgtgct gctgtgggtg cttccggtag
    44101 tggctattgg cattggcggt tgggtcatat acgcccgctc gcggcgtcgg gtacgcgtag
    44161 tgccggaagc gtttcctgaa caaagcgtgc aggaaggtaa gcgtgccgga tatattgttt
    44221 atctgccggg tattgtggtg gcgttaattg tggctggcgt cagctactac cagactggca
    44281 attatcagca ggtgaaaatc tggcagcagg ccacggcaca ggctccggcg ttgctggaca
    44341 gggcgctgga tccgaaagcc gatccgctca acgaagaaga gatgtcgcgt cttgcgctgg
    44401 ggatgcgtac tcaactgcaa aaaaatccgg gagatataga aggctggatt atgttgggcc
    44461 gcgttggcat ggcgctgggt aacgctagta tcgccaccga tgcatacgct actgcgtatc
    44521 gcctcgatcc gaaaaacagc gatgccgcac tgggttatgc tgaagcgttg acacgttcat
    44581 ctgatcccaa cgacaaccgc ctcggcggcg aactgctgcg ccagttggtg agaagcgacc
    44641 acagcaatat ccgtgtgtta agcatgtatg cgtttaatgc ctttgagcag cagcgatttg
    44701 gcgaagccgt tgccgcgtgg gagatgatgt tgaaactctt acctgccaac gatactcgcc
    44761 gtgcggtgat tgaacgtagt atcgcgcagg cgatgcaaca tttgtcgccg caggagagta
    44821 aataaaaaga ggcccgatgg tgatgccatc gggctatttt tattgtgccc cgcgttgttg
    44881 caggaacaga atggtggctg ccacgcgtga gcggacattg agtttgcgca gcagattgcg
    44941 aatatgtact tttactgtct gctctgaaat attcaacacc gaggcaatct gtttatttga
    45001 cagcccctgt gccagctcgt gcagaacatc cagctcgcgc tccgtcagca cgctgaaggg
    45061 atcttcttcc gcgccaaaca tttcacgttc acgtaagtac tgattgacgc gttcgctaaa
    45121 gactttgctg cctttcgctc cagcacgaat tgcttccagc aatacttccg ggtcgctgtc
    45181 tttcaacaga taaccgtctg cgcctgcgtc tatcagcgca aagacatcgc tggaggcatc
    45241 tgatacggtc aggataataa tttgcgcggt aacgccatcc ctgcgcaagg cattgagggt
    45301 atccaggcca ctcatacctt tcatattgag atccagcaag atcacgtcga tatccagtct
    45361 attcgccaga tcgatcgcgc tcgcgccgtc gcccgcttcg gcgaccactt caaagccagg
    45421 atcaagctcc agtaactgac gaacaccgcg tcgcataagt ggatgatcat ccacaatcat
    45481 cacctgaaaa ggcgttgctt caggcatagt agtctcctga ggttttatta gaatcattat
    45541 gtttgagcgt agcgttggta acgctaccag gcgcgtgaat gttaccccat tttatgtatg
    45601 gacatgtagt gaaaactaca accttgtcgt tcgctttggc atcattttca ttataaagac
    45661 ggaattttgc atcagcttct catcaagaaa aacaactaaa aaatcaatca caatacttgt
    45721 gcatttcttt aaacagtttt taaaaactga ctccgggtat ggagctatgg gtattttctg
    45781 tacccaatgc ttttaactgc aattaatttc ataggatgaa agctcaatgc atcaatctgg
    45841 ttctgtttct ctttgtcgtt ccgcaatatc tgttctggtg gctacagcgt tatattcacc
    45901 catagcattg gcatcaacag ttgagtatgg tgagacagtt gatggtgtta tcctggaaaa
    45961 agatatccag ctggtttatg ggaccgccaa taatacgaaa atcaatcctg gcggagaaca
    46021 gcatataaaa gaatttggtg taagtagtaa tactgaaatt aacggagggt atcagtacat
    46081 tgaaatgaat ggtaccgcag aatactcagt attaaatgac ggttatcaaa ttgttcaaat
    46141 gggtggcgcg gcaaaccaga ctaccctcaa taatggtgtg ctacaggttt atggcgcagc
    46201 gaatgatccc acgattaaag gcgggcgctt aatcgttgaa aaagatggga ttaccgtcct
    46261 tgccgctatc gaaaagggag gattactgga ggttaaagag tggggattag cgattgcggt
    46321 agatcagaaa gcaggcggtg ctattaaagc aagcacgcgg gtcatggagg tattcggaac
    46381 aaaccgtctc ggtcagttcg aaatcaagaa tggtattgct aacaatatgc tgttggaaaa
    46441 cggcggaagt ttgcgagttg aagaaaatga cttcgcttat aatactactg tagatagtgg
    46501 cggcttactg gaggttatgg atggcgggac tgcaactggc gttgataaaa aagcaggcgg
    46561 aaaattaatt gtctcaacga atgcgctgga agtgagtggt acaaacagta aaggccaatt
    46621 tagtataaaa gatggtgtgt caaaaaatta tgaactggat gatggttccg ggctcattgt
    46681 tatggaggac acgcaggcca ttgatactat ccttgatgag catgccacta tgcaatcgct
    46741 gggaaaggat actggtacga aagtgcaggc aaatgaggta tatgatctcg gtcgatcaga
    46801 ccagaatgga agtatcacgt attcctctaa agccatctct gaaaatatgg ttatcaacaa
    46861 tggccgcgct aatgtctggg ctggcacaat ggttaacgtg tcagtcagag ggaatgatgg
    46921 cattcttgag gtcatgaagc cgcaaataaa ttatgcaccc gctatgttgg tgggtaaggt
    46981 agtggtttct gagggcgctt cttttagaac gcatggtgcc gtggatacca gcaaagcgga
    47041 cgtttcgctc gaaaatagcg tatggaccat cattgccgat atcactacga cgaaccaaaa
    47101 caccctcctc aacttagcca accttgcgat gtctgacgca aatgtgatta tgatggatga
    47161 gccagtgact cgttcatcag tgacggcaag tgcggaaaat ttcattacgt tgaacaccaa
    47221 taccctgtcg ggaaacggca atttttatat gcgtaccgat atggctaatc atcagagcga
    47281 tcagctcaac gtcaccggtc aggcaacagg tgatttcaaa atattcgtga cggacaccgg
    47341 tgccagcccg gcagcaggag atagccttac actggtaaca acgggcggcg gtgatgctgc
    47401 atttacgttg ggcaatgccg gaggcgttgt tgatatcggt acgtatgaat ataccttgct
    47461 ggataatggc aaccatagct ggagtttggc agagaatcgc gcgcaaatta ccccttcaac
    47521 cactgatgtg ctgaatatgg cggccgcaca accgctggta tttgatgcag aactggacac
    47581 cgtgcgtgag cgtcttggta gcgtaaaagg cgttagttac gatacggcga tgtggagttc
    47641 ggcaattaac acccgcaaca acgtgaccac tgatgcggga gctggttttg agcaaacatt
    47701 gacgggcctg acgctcggta tcgatagccg tttctcccgt gaagaaagca gtacaattcg
    47761 cggcttgttc tttggttact ctcattctga tattggtttt gatcgcggcg gaaaaggtaa
    47821 tatcgatagc tataccctgg gggcttatgc cggttgggag catcagaacg gtgcctatgt
    47881 tgatggggtg gtgaaagttg accgttttgc caacaccatc catggcaaga tgagtaatgg
    47941 ggcaacagcg tttggcgatt acaatagtaa cggcgcgggt gctcatgttg agagcgggtt
    48001 ccgttgggtt gacggattgt ggagtattag accctatctg gcctttaccg gctttaccac
    48061 agatggtcag gactacacgt tatcaaacgg catgcgcgct gatgtgggaa atacccggat
    48121 attacgcgct gaagcgggaa cggcggtaag ctatcacatg gacctgcaaa atggtacgac
    48181 gctggaaccc tggctgaaag ccgccgtacg tcaggaatac gccgattcta accatgtgaa
    48241 agttaatgac gatggcaaat ttaataatga tgtggctgga acccgtggcg tttatcaggc
    48301 tgggataagg tcatcgttta ccccgacgtt aagcggtcat ttgtcagtca gctatggcaa
    48361 tggcgcaggg gtagaatcgc cgtggaatac tcaggcgggt gtggtctgga cgttctgata
    48421 acaaaaaata aacaggctgt gatgtgtcac ggtctgttta tcgaattaat tgcagatata
    48481 aaaaaaacca accgtaaagg ttggtttttt cttgggattt ttggtcggca cgagaggatt
    48541 tgaacctccg acccccgaca ccccatgacg gtgcgctacc aggctgcgct acgtgccgac
    48601 tcgtggctgc taatactacc gttttccaca ccgattgcaa gtaagatatt ttgctaactg
    48661 atttataatt aatcagttag cgataaaacg cttctcgtct gtcagcactt gcaataacaa
    48721 acttaactgt ggtttttcat ctttcactct ttcaccacgt aagttgtaag tgcggtattt
    48781 accgttattg ttcagcacca gcgtcttttt cggggtggta attgccagcg tatcgttatc
    48841 cgctgcagta acccagtaat gacggcgttg agggttgaac aaatcctgac cttgcgaata
    48901 ttcgctggca ggtgtgctga catgtagcag gcgttgcatc agcgtcgtca tcagatcggt
    48961 atgatcagtc agagcattaa tacgctgcgc cggcgtgcct ggccagtgaa tcactaatgg
    49021 cacctgcaga tgaccgtggg accagtcaaa ggtttcttcg tcttcgctca gcggaatacc
    49081 ccgaccggca gtgataatca caaccgtatt gtccagtttg ccagaatcac gcagtgcatt
    49141 gagcacgcgg ttgatctggt catcgacatt gcctgccgcc cggctatatt tccgtgcaaa
    49201 tgcctgctga ttgctgtcgt caatgttagt gccattgaaa gagacccacg agaaccagcg
    49261 gttatcttct tgtgcgtagc ggcccagcca gttgatccac tgcgtggcgg tctgctcgtc
    49321 ggattgggtg cgtacgctcg gcatcgagaa atctgacaac aatgcctggc gatacagcgg
    49381 gctggtaaag ccatctgatg agaataatcc cagctgatag ccttgctgat taagcgcagt
    49441 aattaacgcc gcaggggtac gggtcgacag aatgccgtcc atatagctcg gcgagatgcc
    49501 atagaacagg ccaaagatgc cgttgtctgt agtgttgccg gagctcatat ggcgcgtgaa
    49561 cgaaatattt tgctcagcaa aacctgccag cgcagtcatc tgcttctcga agcgtgagta
    49621 gttcaggcca tcgacagtaa tcaacagcac attctgcccg gtgcccatat cgcgatagcg
    49681 cagttcgctt aacggatact gaacggaaac ggcgtctgga ttaccttgct caataagacg
    49741 gcgttgatac tcctgcgcat caagcagacc atgcttctca agaaaacgtc gcgccgtcat
    49801 cgggtacgaa agcggcaggt tagcgcgctg catggtgatc gggcgataga agttggcatc
    49861 ggcccagata tacaccacat gcgaggcgat aaaggcgata aataagaatg cggccagcgg
    49921 gcgcgcgaag cgccgacgac gcgtcaggct gcgcagcttt tgccagctcc acgtcgcaaa
    49981 caccagttca agcaataaaa taaccggcac gctgatgaac atcagctgcc agtcgcgcgc
    50041 catctcattt tcgtctgggt tgataaccag ttgccagacg atgggattaa gatggagatg
    50101 gaaacgagta aagacttcgc tatcgatcag taatagcgtc attcccgccg ttgccagaat
    50161 gacggacaaa aacctcatca gcctctggga gccgacgata aaggtcagcg ggaagaggat
    50221 cagcaagtag gtggcgaaca ccaggaagct gaaatggccg ataatgctta cgtaggaata
    50281 aatgcgacca gcaagcgttg tcggccagtc ggcgataaac aggtaacggc tgccaatgac
    50341 gagcgaaagc agaatattga acagtgcaaa ccagtgcccc caactgacca tctgggagac
    50401 tttttcacgg tagcgctgac gatgagttac cataacctgt tatctgtttc ccttagtgcg
    50461 ctttgtcttc gttgatagag gactgtaagg cgcgggcaaa agagttggca attgcctggc
    50521 gttgggccgg ggcaatgctg gtgttgataa ggttagtgac catgttgcct aacaccatca
    50581 gggaaaggtc ggtcggagcc ttatgttttt ccagtacgtt gagcagctct gcgagcaatt
    50641 gttcaacctg ttcatcactg tagcgggaaa tttgtggcat aaatcgaaat cagttagttg
    50701 atgaaagggc aacatattac cgtagcaacg gttttttttc tgctttttta tgcgttaaat
    50761 cattctcgtt acgctgcctt ctgtaaaatg accgccatct tttattgcgt cgcttaaccg
    50821 gcggtggttg aataccgccc ggtcttaaag gagagtttat catgagtctg gatatcaacc
    50881 agattgccct gcaccagctt atcaagcgtg atgagcagaa ccttgagctg gtgttgcgcg
    50941 attcattgct ggaaccgaca gaaaccgtcg ttgagatggt ggcagaactg catcgggtct
    51001 atagcgccaa aaataaagct tacggtttgt ttagcgaaga gagtgaactg gcgcagacgc
    51061 tgcgcttaca gcgtcagggc gaagaggact ttctggcatt tagccgcgcg gcaaccggtc
    51121 gcctgcgtga cgaattagcg aaatatcctt ttgctgacgg tggattcgtg ctgttttgcc
    51181 actaccgtta tctggcggtg gagtatctgc tggtggcagt cctgagcaac ctgagcagta
    51241 tgcgcgttaa cgaaaatctc gacataaacc caacccatta tcttgatatc aatcatgcgg
    51301 atattgttgc gcgtatcgat ttaaccgaat gggaaaccaa tccagagtcg acccgctatc
    51361 tcactttcct gaaagggaga gtagggcgca aagtggcgga tttctttatg gatttcctcg
    51421 gggccagcga agggttaaac gcgaaagcgc aaaaccgtgg gctgttgcaa gcggttgatg
    51481 atttcactgc cgaagcacaa ctggataaag cagagcgaca gaatgtgcgc cagcaggttt
    51541 atagctactg taatgagcag ctgcaggcag gtgaagagat tgaactggaa tcgctgtcga
    51601 aagagctggc aggcgtaagc gaagtgagct tcacggagtt tgcggctgaa aaaggctacg
    51661 aactggaaga gagcttcccg gcagaccgta gcacactgcg tcagttgacg aagtttgccg
    51721 gtagcggcgg tggcttgact attaactttg atgcgatgct gttaggtgaa cgcatcttct
    51781 gggatccagc aaccgatacc ttgaccatta aaggcacgcc gccgaatttg cgcgaccaat
    51841 tgcagcgccg gacgtctggc ggcaattaat cggttttgtg ttctgaatgc cagatgcggc
    51901 gtgaacgcct tatctggcct gcaaaaaata gcaataccgt aatcaggcat aatctgcaga
    51961 tacaaaaaac cccgccggag cggggttttt tacaacttat tcagcaatta agcgcgaacg
    52021 aagtcgatgt gctgcagctt cggtttgtac gggtgacgct gtacgtcctg agctttaact
    52081 ttgatttctt taccgtcaac aacgatggtc agaacttcgc tgtagaattc agctttagct
    52141 tgcatgttca tgactttgtc gtgatccagc tcgatagcca gcggcgcttc tttgccaccg
    52201 tagatgattg ccgggaactt gttagcgaca cgcaggcggc ggctcgcacc cttaccctgc
    52261 tctttacgta cttctgcgtt gatagtaaac atttctttct ctcttaaatt aatcctgcta
    52321 caggcgaccc agcaacaggt aagtgatctg ctttgcgtat gcaaaagcgg gcgagattct
    52381 atactcaatc gccttataca tcaatcaaaa ctaccattaa ccgcgtaatt cgtgcgcccg
    52441 acgaaaacga ccttcgtaat cgaacacttt ttcacgcact tgccagtact gacctttcat
    52501 gcgggcgacg acaaaatccg gatgtcgcag taaggcttgc tgggcgagga tatcggcggc
    52561 ggtgatccag cgcagaggga tgcccggtgt gcgcgtatgc gggcggataa aaagctgctc
    52621 gaaggcggta cgctgggcgg gcgtttgcag acggaaacgc tcactcacat ccgcgccgtc
    52681 ttcatcgtaa taggtgattt tcaaccattc gcctttctcg tcatgcccat gctgtaaaga
    52741 catgccgcta cagcgtaata ccagcgcgtc tttcagtcgt agcgccgctt ttaacatatc
    52801 gtctggatca accagtacgg tgtcacattc gcggcagcgg cgagcggcaa tatcattttc
    52861 tgcgttacat tgcgggcaat ttttaaaacg gaaacggaag tcacactgtt cgcgatgacc
    52921 gtcgtcatct tcaaaccatc cctgacagcg acgaccaaag tgttcaatca atgtcccgtc
    52981 ggcggtcgtt tttccccaaa aggtgttggc aaaaccgcag acagggcaga aaacctgtac
    53041 cggaacgttg tcacttttgc ctttcggtgt accaacttcc ggcgcgtaga gatcgtgagg
    53101 attacccgca taatcaagaa ttaaacaatc agtcttgccc ggagcgagac gcagaccgcg
    53161 cccgacaatt tgttggtaaa gactcactga ttcggtaggg cgcagaatgg cgataagatc
    53221 gacgtgcggg gcgtcaaatc cggtggtcag tactgcgacg ttgaccagat agcgaaaacg
    53281 ctgggcttta aaatcttcaa ttaacacatc gcgctcagcg ccgggggtgt cgccagtaat
    53341 cagtgcagca tcttcggcag gcagtaatcc cacaatctct tttgcgtgtt caacggtggc
    53401 ggcgaaaatc attacccctt tgcgcttttc ggcgaactcc atgatctggc tgatgatatg
    53461 tggcgtaatg cgttgctgtt ttttcagctc acggttgaga tcagcttcgc tgaacagccc
    53521 gttactctgt gcctgcaagc ggctgaaatc gtattgcact actggcatat ccagtcgttc
    53581 tggcggcgtc agatagccgt gtttaatcat ataacgcagc ggcagctcat aaatgcagtc
    53641 acggaaaagg gctttctcat cgccgcgtac catgccgtga taatggaact ggtagatcca
    53701 gcctttgccc aatcgaaaag gcgtggcagt cagccccagc aggcgtaagt ggggattcac
    53761 tttagtcagg tgagtgagga tttgctgata ctggctctct tcatcgtcac caatacggtg
    53821 gcattcatcg acaatcaaca gcgaaaattc accctgaaag gcatcaagat tacgagcgac
    53881 cgactgcacg ctgccaaata ccactttacc gtggctctct ttgcgcttta acccagcggc
    53941 aaaaatatcg gcttccagtc ccagcgcctg atactttgca tggttttgcg ccaccagttc
    54001 tttgacgtgt gccagcacca gaacacgacc acgcgccagc cgtgccagtt cggcaatcac
    54061 caggctttta cctgcgccgg tgggcagcac gataacggca ggggttttat gacgacgaaa
    54121 atggttgagc gtggcatcca cggcttcttg ctgatatggg cggagtgtaa aaatcatggt
    54181 ctcgcgtagg tagattattc tgctaatagt atgccacgga tctttttccc ttgagggatg
    54241 ttattagacc gctatactgg gcagacaaca gttcagattt tcgggtcatt acccgacctc
    54301 cgttttttac aggcaaaatc acacacatgc gacttgataa atttatcgca cagcaactcg
    54361 gtgttagccg tgctattgcc gggcgtgaaa tccgcggcaa tcgtgtcacc gttgatggcg
    54421 aaatcgtccg caatgcagcg ttcaaactgc tccctgaaca cgatgttgct tatgatggca
    54481 atccgctggc gcagcaacac ggcccacgtt atttcatgct caacaagccc cagggctatg
    54541 tttgctcaac tgatgacccg gatcacccaa cggtgctcta ttttctggat gaacctgtgg
    54601 cgtggaaact gcacgcggcg gggcggttgg atattgatac caccggtctg gtgctgatga
    54661 ctgacgacgg tcagtggtcg catcgcatta cttctccgcg tcatcattgc gagaagacct
    54721 atctggtgac gctggaatca cccgtagctg acgatacggc agagcaattt gccaaaggcg
    54781 tacagctgca taacgaaaaa gatctcacta agccagcggt gctggaagtg attaccccta
    54841 cgcaggtccg tctgaccatc agcgaagggc gttatcatca ggtgaaacgc atgttcgccg
    54901 ctgtgggtaa ccatgtggtt gagctgcatc gtgaacgtat tggcggtatt acgctggatg
    54961 ctgatttagc ccccggtgaa tatcgtccgt taactgaaga agaaattgcc agcgtcgtct
    55021 aacttttcca tgactttcag gagcccgttg tgaccacccg acagcattcg tcgtttgcta
    55081 ttgtttttat ccttggcctg ctggccatgt tgatgccgct gtcgattgat atgtatctgc
    55141 ccgcgctacc ggtaatttca gcgcagtttg gcgtacctgc gggcagtacg cagatgaccc
    55201 tcagtactta tattctgggc tttgcgttgg ggcagttaat ctacgggccg atggcagaca
    55261 gctttgggcg taagccggtg gttcttggcg gtacgctggt gtttgccgcc gccgcggtgg
    55321 cgtgtgcgtt ggcacaaacc atcgatcagc tgattgtgat gcgtttcttc cacgggctgg
    55381 cggcggctgc ggccagcgtg gttatcaacg ctttgatgcg tgatatctac ccgaaagaag
    55441 agttctcgcg gatgatgtcg tttgtcatgc tggtgacgac cattgcaccg ctgatggcac
    55501 cgatagttgg cggctgggtg ctggtgtggc tgagctggca ttacatcttc tggatcctcg
    55561 ccgtggcggc gattctggct tcggcaatga tcttctttct gatcaaagaa accttaccac
    55621 cggagcgtcg ccagcctttt cacattcgta ccactattgg taactttgcg gcgctgttcc
    55681 gtcataagcg tgtcctgagt tatatgcttg ccagtggttt cagctttgcc gggatgttct
    55741 cgttcttaag cgccggaccg tttgtctaca ttgaaattaa ccacgtcgcg ccggaaaact
    55801 ttggttatta ctttgcacta aacattgttt tcctgttcgt gatgaccatt tttaacagcc
    55861 gcttcgtccg ccgtattggc gcgttaaata tgttccgctc ggggttgtgg atacagttta
    55921 ttatggcggc gtggatggtc atcagtgcgc tgctggggct gggattctgg tcgctggtgg
    55981 ttggcgttgc ggcgtttgtg ggctgcgtgt cgatggtgtc atccaatgcg atggcggtca
    56041 ttcttgatga gtttccccat atggcgggaa cggcatcttc gctggcagga accttccgtt
    56101 ttggcatagg ggcaattgtt ggcgcattgc tttctcttgc gacctttaac tctgcatggc
    56161 cgatgatttg gtcaattgca ttctgcgcaa gcagctccat tctcttctgt ctgtacgcca
    56221 gtcgaccgaa aaaacggtga tctattgcac aacgaggaag ctaaaaggct tcctttgttg
    56281 atgcatgtca accacaaatc tatcattccc ccgatatatg tttattttat gtaaaatcaa
    56341 tttatgtaaa aagtcacatc attgtagtta aaaaggttga gttagatcgc agaaacgggt
    56401 acatatagcc ccgcaaacgt gaccacgccc gcagatatta cttaaatcag agccatagag
    56461 gccacgcagg cgaggcatca atctttacga tctgtataaa gacggattgt tgatgatgtg
    56521 ttaaaattga tgtaaacaaa ttgtgaagtg aatgtgcttc cggggaaaat aagtgacttc
    56581 attacaactc tcaatcgtcc atcgactgcc gcagaactat cgctggtctg ctggtttcgc
    56641 aggttcgaag gttgaaccga ttccgcaaaa tggaccgtgc ggtgacaaca gcctggtggc
    56701 gcttaaattg cttagcccgg atggtgataa tgcatggtcg gtgatgtata aactaagcca
    56761 ggcgttaagc gacatcgaag ttccatgttc ggtgctggag tgtgaaggtg agccgtgcct
    56821 gtttgtaaat cgccaggacg agtttgctgc aacatgccga ttgaaaaatt ttggtgtggc
    56881 aattgccgaa cccttttcaa actacaatcc tttttaagcg tcagctcaac gccagtagct
    56941 gacgcgtata ctcctgctgc ggtgtggcaa acacgcgcgc gcacggtcct tgttccacta
    57001 cttccccttg tcgcagtacg ataacctgat gacataacgc gcggacaacg tgcaaatcgt
    57061 ggctgataaa caaataggcc agttgatgct tttgttgcaa tgatttcaat agcgtcaata
    57121 tttgcgcctg taccgttttg tcgagtgatg atgtcggttc atcaagtatg atcagcgagg
    57181 gcttaagaat taatgccctg gcaatcgcaa tacgttgtcg ctgaccacca gagaactccg
    57241 ccggataacg gtggcgtgtt tcaggatcta atcccacttc atgcatcacg gctatcactt
    57301 gttgttcgcg ttgtgcggca gaaagcgtcg gctggtgaac ccgtaagcct tcctcaataa
    57361 tctgcaaaac gttgagtcgt gggttgagcg aggagtttgg atcctgaaat accacctgaa
    57421 tgcgatgacg aataggtaac agctggcggc gatttaaatt ttgcagtggc tgaccgtcaa
    57481 agatgatgct gccctgagaa ttaatcagtc gcagcagcgc cagtcccgtc gtacttttcc
    57541 cggaaccgga ctcgcccact aaacccagtg tttcacccgc tcgtagcgta aaactgatgt
    57601 ttttcaccac cacattatga tccacaatgc gcttcaaaat ccctttgcga atggggaagg
    57661 caacctgaag ctgttcaaca tccagcaacg ttgaggcagg ttctggcaac ggcaccgggt
    57721 cgcctgacgg ttcactgttg agtagctttt gtgtgtaagg atgagtgggt gaggcaaata
    57781 gcgtagcggc gtaattttgc tcgacacagc gaccgttttg cattaccgcc acgcggtggg
    57841 ccagttttct gacaatgctg aggttatgag taataaacag catgcccata ttcagctcgc
    57901 cttgcagttc gcgcaacagc tgtaaaatct gcgcctggac agagacgtcc agtgcggtgg
    57961 tcggttcatc ggcaattaat aattccggtc gcgttaacag cgccatcgca atcatcaccc
    58021 gctggcgttc gccgccggag agctgatgcg gataatctgt cagccgtttt gccgcctggc
    58081 ggataccaac gcgatcaagg cagttaagaa tttcgcctcg agccgcttcc cgacgcatcc
    58141 cgcggtggag tgaaagcact tcataaagct gtttttccag ggtatgcaat ggatttaacg
    58201 acaccatcgg ctcctgaaaa atcatggcga tcttattacc gcgtacaccg cgcaacgttt
    58261 gatcgctggc atgaagcagc gattcgccat gaaaacgaat atcgccggag agatattcaa
    58321 ccggcgggga agggagcagg cgtaaaattg acagcgcggt aacgcttttg cctgaacctg
    58381 actcacccac cagcgccagc gtttcgccag cctcaatctg tagtgaaaca tcattgacta
    58441 ctgtacgtac ggtttgctga tggcgaaaac ccaccgacaa attttcaatc gctaacagag
    58501 tttgcgtcat gctacaccgc cttattagga tcaaatgcgt cgcggacggc ttcaccaata
    58561 aagatcagca aagacaataa tatcgccacc gacaagaagg cggtgatccc aagccacgga
    58621 gcctgaaggt tatttttccc ttgtaacagc agttcaccga gtgacggtga accgagcggc
    58681 agaccgaagc cgaggaaatc gagtgaggtc agggtggtta tcgaactaca taaaataaac
    58741 ggtaaaaagg tgagggtcgc gaccatggca ttaggcaaca tatgacgcag gatgatactg
    58801 cgatcgctga cgccaagcgc ctgcgccgca cgaatgtagt cgaaattacg ggtacgtaaa
    58861 aactccgccc gcaccacgcc gaccagactc atccagccaa acaagacagt aattgccagc
    58921 agccaccaga agttaggctg tacgacgctg gaaagtaaaa taatcaaaaa cagcgtcggc
    58981 atccccgacc atacttcaat aaagcgttgc ccccagagat cgactttacc gccgtaatag
    59041 ccttgtagcg cccccgccag cacgcccatc acgctggaac agagagtcag catcaggcca
    59101 aacagaaccg agatccgcgt gccatagaga atacgtgcca gcacatcgcc gccgttggca
    59161 tccgttccca gccagttttg ccgggagggt ggagaaggga agggcttatc ggtagcaaag
    59221 ttgatactgg tagcaccaaa gcgaatcggt gcccacagta cccagccgtt attttccagc
    59281 cgttgtttca gccacgggtc ctgataatca gtctgacttg ccagcgggcc gccaaaatcg
    59341 ctttcgctgt agtttttcaa caacgggaaa taccaactgc cgtcataacg taccagcaac
    59401 ggtttatcgt tggcgataag ttcagaacac aaactcaaac caaacaaaac gaggaaaatc
    59461 cataacgacc agtagccgcg acggttatgg cgaaaacgcg cccaacgggc ctgattgacg
    59521 gggctgagtc gcgacattaa cgtccctcaa aatctatacg cggatcgacc agcgtatagc
    59581 tgatatcact gacaatattc agcagcaggc caatcagggt gaaaatatac aaggtaccaa
    59641 acattacagg atattcgcgc gagacggtcg cttcgtagcc cagtaagcca aggccattga
    59701 gtgaaaacat cacttcaatc agcagcgagc cggtaaaaaa catgctgata aacgtcgccg
    59761 gaaaaccggc aatcaccagc agcatggcgt tgcggaacac atgtttccag agaatatttt
    59821 tttcacttac ccctttcgca cgcgcggtca ccacgtattg cttgcgcact tcgtcaagga
    59881 atgagttttt ggtcagcatg gtcagcgccg caaagccacc aatcactgtc gccagcaccg
    59941 gcagcgtgat atgccacaga taatcggtga ttttctgata ccacggcagc gaatcaaagt
    60001 tagcggaaac caggccgcgt agagggaaca ggtcgaaata gctgccgccc gcgaagaaga
    60061 caatcagcag gatggcaaac aaaaaggccg gaatggcgta gccgatgatg ataaatgcgc
    60121 tactccagac gtcaaagcgg ctcccattat aaacagcttt gcgaatgcct aacggaatcg
    60181 acaccagata gataatcagc gtgctccaca atccgagggt gatggaaacc ggcagactgt
    60241 ctttaatcag cgtcagcacc gaggcgctgc gaaacaggct atcaccaaaa tcaaagcgga
    60301 tgtagtccca gagcattttg aagtaacgtt cgtggatcgg cttatcgaaa ccgtagcgat
    60361 gagtgatctc agcgatcact tctgggtcta atccgcgtcc gccacggtaa ttactgtcgc
    60421 tgatattgcc gacacccgtt tgcgcatggc tggcacgaac accttcaccg cctgcgccgg
    60481 gtaatactcc ggcattacca aactcaatgg cggcgatggc ctgatcgacc ggaccgccag
    60541 gcgcaatttg cacgatgaaa aagttgatag tgataatagc ccataacgtt gggatcacca
    60601 gcaacagacg gcgaatcagg taagcgccca tctactctcc ctgttgtctg gctgacggca
    60661 gtttggccgc tttattaacg tcataccacc aggtatcgat acccaggctg taaacagggc
    60721 gtacagcggg ttgggagaat ttatcccacc aggcgagacg gtcttccgcc atgtaccaca
    60781 ttggcagcat gtaataattc cacgttaata cgcgatccag tgctcgcccc aacggcagta
    60841 atttttcttt atttccctgc gcggcaataa tttggttgat cagcgagtcg ataaccgggc
    60901 tttgcacgcc gggggcatta taagtggaat tgatatattc cgatgaccag gaaatctgta
    60961 aatcggaact gggccacggc atcgcccgcc ataggcgcgg catcatgtca tagtcgcgac
    61021 tgcgcatgcg gttagtgatt tgcgagttat ccaccttgcg aatgtccata ttgataccca
    61081 gccgttgcag gctgtgctgg aacggcaata cccactgact attgctgctt gcgggaagca
    61141 ataattcaaa gctgagcggc tgacccgttg tggcattaac gcgttgctga cccttcagca
    61201 cccagcccgc ttcgttgaga agtttgtcgg cttttaacag gttgtcacga tcgtagccat
    61261 cgcctttaga taccggcggc tggtagattt gtgtgaagac ttctggcggt agatcttttt
    61321 tcattggtgc cagaagcacc agctccgcgg cgtcggggta atttctggcc gcgtattcgg
    61381 tattctgaaa ataactgttc gtgcgactcc aggcgttgta aaacaacgcc ttgttcatcc
    61441 attcaaagtc aaaagcgaga gtgattgctt cccggacccg gcgatcgctg aataccggac
    61501 gttggatatt aaacgccagc cagcgcgtat cctgggctga ttcgttcttt tgctcatctt
    61561 tgatgatgta ttttttatcg aaatttttac cggtataacg cgtggcccag tttttagcgt
    61621 cgttttccat acgcaaatca aaggcacctg ctttaaacgc ttcaaaggcg acattatcat
    61681 cgaggtaata atcgtagcga atggtgtcga aattccagcg tccacggttt accggtaagt
    61741 ttgctgccca gtaatctttc acgcgggaat agacaatatt ttgccccatt ttccaggacg
    61801 taacgcggta cggaccactg gcaagcggag gcgtggcgag cgggtcgcta agtttgtgat
    61861 ccttccagta cttttctgga aataccggca gcgaaaacag actcagcata tcttctttgc
    61921 tgggtttagc taactcaatg cgcacggtta acggtgcaat ggctttgacg gtggtgcctt
    61981 tgtagaccag acgaaattgc ggcacgcctt cggtcataaa tttttgaaaa gtaaactcta
    62041 catcgcgggc agtaatgggc gaaccatcat gaaaacgggc gcgcggatta atagcgacct
    62101 ccacccagga ataatcgtca gcatagcgtg cgctttcagc aatcagcggg taataactgc
    62161 ctggttcgtc atcggaagtc gtaaatagcg tgtcgtacag ctgctcggtg cgtgcgcccg
    62221 ggttgccgcg cagtgcatag cggttgaaat tatcgaaggt gccgagggct gacaacgtta
    62281 tctgcccacc ttttggcgcg gcggggttca cataatcaaa atggttgaaa ttaaacgcgt
    62341 accggggttc gcccagcacg gcaaaggcat agctttcctt gatagcctgc gcctgcacac
    62401 caaaggtgaa cagagcgata aacagcagca gtatgcgcac aatcatctgc aacggatttc
    62461 cttttactca tcattagccg caatccggca tgctttcact atgaataata agggacgtaa
    62521 gcacacctta ccactgcggc gtatacggtt tcttcagcca gcggacaaaa tcgtccagcg
    62581 gtaacgggcg actaatccag tagccttgca tgaaattaac gccgcgttcg cttagccatc
    62641 gcgcttgttc cggcgtttcg accccttcag caactgtcag catattgaga cgtttcgcca
    62701 gcgtcagcac cgcgtcaagt acgggtgaag tgatcgtttc cgtaccgatg gcgttgataa
    62761 atccacggtc aattttcaga taatcgagcg taaaacgctc aagatagata agcgcgctgt
    62821 gcccggtgcc gaagtcatca atagcaattt ctacgccgac cgagtgcagc caggcgaaga
    62881 gttgtgtggc ttcttgctct ttcagcatat cgcgctcggt aatttccagc acaatctgga
    62941 aatggtgtgc gggtagggaa gtgagcagtt tctggatatc tgctttaaag ctttcgctgt
    63001 gcagatggtc cggcgcaatg ttaataccaa atttgacgcc tactggcagc actttttcta
    63061 attctgcggc atcgcgggca attaactcaa acaggtgctg agtcagcggc acaatcatct
    63121 tttgcgattc ggcaaagtga atgaaggcat ccgggggaat ttctcccgcg acaggatgcc
    63181 gccagcgtag cagtacttcc aggcccgtta ctcgcaaagc ttgtgtatcc accaccggtt
    63241 gatacgccac gtaaaattgt tcgcgcttga tggcggtcat gatttctctg ccggggcgca
    63301 tacgtacgct catcaggtaa tagcagagca ggccaacgac agttccactc atgccgccca
    63361 gtaaaaatgc gtaccacaca tcgttccatg tccagtcatc cgcataaagc cgaacggtca
    63421 gaggaatgcc cgcaatttta gtttcacgga ccggcatgtc ggttaattca ttaacgttca
    63481 tcaaacgtga agaaaaggta gatagcgcag tattgccaat aatgagggcg acgccatcgt
    63541 aatcttcctg ccgtgaacta taaaagagag aaggcgtcag gttgagattc agagcggcaa
    63601 acacaccgct atttttcagc aaagggttgc gataccagat gacgattgcg ggtttgttcg
    63661 gcaccatcgg cgtgccgggt aagatcgcca tatcgacgtt tttattaatg tcgagcgccg
    63721 gaatcaattc attgagtgga atgtccatct caccagtcgc agatgaacag aatgtttttt
    63781 tatctttcac cagcacaaac gtacggacat tcatgctaaa cgccgcgcga gcggtcagtt
    63841 cggggttagc ctgctggcag gtatctaagg tcagcggctg aagccggtca gtagtggatt
    63901 tcaggtcggc aaaataggta tcgagatact tttgtacgtc gataatcagt gtgtcgtatt
    63961 tgacttcgtg cttatgccag gccactaaaa attgaaggca actcaccagt atcgcaatca
    64021 tcacgcctgc aacaatgcag gtaagcagga gcttacgtcc agaattggga gcgcgtatga
    64081 acatgattcc gttaatttcc tgaaattacg acaacattta aaagataaca atgaccggac
    64141 tatagcctgg atcaagacgc ggaaacatca ttttctctga tgaatgaaaa caaaaaagca
    64201 ctgcctaagc agtgctttca gttaactcgt caggatagcc aagggattgc atccaaacgg
    64261 tttattagct gcggctgaga acccggcgtg cttcgttgta acgcttcttc cagtacggtt
    64321 cattcatgct ggaaataata acaccactgc tggtggaagc atggacaaac tgattgttgc
    64381 cgatataaat accgacatgg cgtcccgttg aaccggcacg gaacagaact aaatcacccg
    64441 tacgcaaatt actgcgggaa acagatttac ccatttcctg ctgttcgtaa gtcgaacgcg
    64501 gaagttctaa gccaaattgc tcacggaatg tacgttgtac aaaaccagaa caatcgatac
    64561 cttttttagt gctgccgccc agacgataac gtacgccttt ccagtcagca tactgatcca
    64621 taattcgcga ttttacgtcg acattacgaa ccaggttttc aaattcatcc tgagaagctt
    64681 gcagtgatga tgtttcacta cccactgcac gtgtctcagg atgcatattc tttgcggtgt
    64741 tatttgcact acatgcagaa agcagaaccg ctactgcaat cgcggggatc ccgcgcaaga
    64801 tatatctcaa aatcggttga gatttgacca tgttgtttgt tttcccttga agtccttaac
    64861 gacaaatatc gttataaaaa atgccaagcg acacgagact aattacctgg gcatgttgaa
    64921 acaattcctt aaggccaaat ctgtggacta acgcacaaaa ttatatgctg tgaggataat
    64981 ctaccgccga ggcaaaacga gttcgagatt acccgattgg tgcggtcttg gcgagcggtt
    65041 ttacatgatt ttttataaga aaaagtgata cagaaagtta ataagcggga ttggtcaatt
    65101 aaagtacgat tgagtgaaac gcatgcaatc cattcattta cttagagaat gatttttgcg
    65161 cgcgatggat gaaagcacgg tgcgcatcat caggattatc ctcactataa aaataagcct
    65221 gatgatgtta attactgtga gttatttgtt ttggaaatgt ttgttttttc ccggtagtga
    65281 tttgtcaaaa aaggtgatta atcgatcact taatggcgtc agcaacaccc agggcaaacc
    65341 gatcaatatc acggtcatcg aaccgacaat gatgtcagta aaccagtgtg cgccaatcat
    65401 tactctggga aatgcaaaaa ccacaaaaat aataagggcg ataaggcctg caactttgcc
    65461 gaaataacgc cacatgaatg ccgaaaaaat aagcagcatc atgccgtgat cgccgggaaa
    65521 gctatctcgt gaggcatctt tcgtgggaac agagagcagt tcgctgacgc ggttaatatc
    65581 ggtaaaagtc aatgttgggc tggcccgttt tacaggaatt aatgcctgac ccagctggtt
    65641 taataccact gcagttaata gcatgaccag acccataatc acgatacgtc gtctgccagg
    65701 ggcgttttct ttcagccaga aactcagcat caacattccc atcgccagca gagaacaacc
    65761 gtcgaaagcg cgattgttgg tcaatgcaac cagccacaaa aaggccttac tttcgaccag
    65821 tttctgatta aagaaataaa aaatatccgc atcaatcggc aaccagaatc catgattaac
    65881 gggaatatac caggaaagaa acagcgcgag gccgacaata ttcaacaaca ctatttgcgg
    65941 caaattttta atcatatttt ctctaattat cttactgaaa aacgcaggca accttacgcg
    66001 gtagtcgcta aacgaagctt caacagcgcg ctctgcaaag cattccagtc agcttcgctg
    66061 ctggaaatca gctcaatacg gctgtccggt ggcgcaacgt tttgcgtttc aatgtgcagg
    66121 tcatcgccct gacggttgat tcgtaccagc ccttcgggaa tacgcagcac gcctttgacg
    66181 cgttccaccg gtgcaagtcg cgcccattcc agaatgccaa tggtgtcgaa taccgtatca
    66241 gcgtcgaata tccagccgca ggcctgatat ccttgcccgc tgttcagact gcgacgccag
    66301 cgttgatgct ctggcaggct taacgctgct aacccttttt tcacgacatg ctgatgagaa
    66361 tgcgcggcgc tggcgggcaa ctcggctaaa ttgcgacgcg gcaaatccag aagatgaccg
    66421 tcaactttcc catgctcact gtgaattaat tgtcgatcgc caccattttg ctgccaccaa
    66481 cgctgtagcg cttgctcact ttcgggcgtc gcacggtcgg atttattggc gacaatgatg
    66541 tctgcggcag ccagctggtc acggaagttt tcattgctgg cgcttttttc gtccagcagc
    66601 aggcgtggat cgagaatgca caaggtggcg cgcagatcta tccacagttc atagactggt
    66661 gcggttaaca gatcgaggat ctgtttcgga tggcccagcc cggtcggctc tatcaacaag
    66721 cggtctggtt ttccctgacg cagtaaggta ttcaacccta cctgcatggg taaaccatta
    66781 acgcagcaca tgcagccgcc ggggatctct ttcagcaatg cgccgctatc ggcgagcaaa
    66841 gcaccatcaa ttccgacttc cccaaattca ttaaccagta ccgcccattt ttcgttggga
    66901 tctttatggg ctaacagatg aagaatcgac gtggttttcc cgctgccgag aaaaccggtg
    66961 atgaggttgg tcctggtcac aacggctccg caatactctg gtaagttaac tgagcgttaa
    67021 tcctggctaa aaaaggcaga aaagagaagg ggagagcgga cgaaggtgag cttcatccgc
    67081 tttaaccgca gaaatcgtta aattttgagg gtgtttaata gcgcctgatg cgcaccgaat
    67141 tgtgagattt gttgccaggc ttgttcgatg gcctgcacaa aatgcgggtt atctggcaga
    67201 tcatcaccga aaacttcacg cagggaaagc agggcggtga cgcgttgctc actgctgctg
    67261 cccgcaacaa gatcgcgaat tttatcgcta agcggatcgc gaacatcaat ggcctttccg
    67321 gcatcatcaa cgccgctgac gtaacgcatc cagcctgcaa cgcctaatgc cagcaacgac
    67381 cagtccgttt cgcgccccag atgtatgcga atacctgcca gcatacgttg cggtaatttc
    67441 tggctgccat ccattgcgat ttgccaggtc ttatgtttca gcgccgggtt agcaaaacgt
    67501 gcaattaact tatccgcata ttgtgttaaa tcgacatctt taatccgcag tgtcggcgct
    67561 tgctcatcca gcattaatgt tctggcggca tggcgaaatg cgcgatcctg catgcaatca
    67621 ctgatatggg cgtatcctga aaggtaaccc agataagcga gaaaagagtg gctgccatta
    67681 agcatacgca gtttcatctc ttcccatggc aggacatcat tcaccatttg tacacctgcg
    67741 acttcccagg caggacgccc agcgacgaag ttatcttcca ccacccactg gataaacggt
    67801 tcgcagctaa tcgcgcaggg atcattcacc cccagatgct ggctgatttc cgccagtgat
    67861 tcgtcggttg ctgccggaac aatgcggtcg accatggttc ccggaaaact gacgtgttct
    67921 tttatccacc cggcgagttc tggcgaacgt ttttctgcca ttcccagcac cgcgtttttc
    67981 accacatggc cattgtcggg aatattgtcg caggagagca cggtaaacgg tgtaaggccg
    68041 cgctcacggc ggcgtttcag tgcttcgacg agaatacccg gtgcggagtg aggttcttca
    68101 ggggtttgta gatcgtgaat aatccgcgga ttgctggtgt cgagtgcacc ggtggccggg
    68161 tcaatacaat agcctttttc ggtaatcgtc agggaaacaa ttgccacctg tggctcgcaa
    68221 aatttctcaa taattgccgc taaggaatcc agttttgcat taaggcattc gtgaacggca
    68281 ccgacaatta tcgcctgatt gccgtccgca cctttctcca gcacggtata taaatggttc
    68341 tgtgcgcgga gctggctcat cagttgatca ccgctgaaca agctgatttc acagatcccc
    68401 cagtcgccgc cctgggcatt cagcacacgc tcggtcagta atgcctgatg tgcgcggtga
    68461 aaggcgccaa agccaaaatg aacgatacgt gattgcaact gctggcgatc gtagcgtgga
    68521 gcgtgtacat gatgtgggag cgtaacggag gcaattgtat tcataggagt cacgtcttag
    68581 taacgattaa ttaacaatga cagtgtaaag ttatatgaca gcaattgaaa ttggtctgga
    68641 ttgttaatgc gatgaagtgt gagggcgatc aatcaacgag tgaatattgc gggcgtaagg
    68701 tgttcaggaa gcgcaaaata gaagattggt ataataaatt aaaaaaggga cagcctgagc
    68761 tatccctttt ctgcatgcgg ctgaagttag tcagcacgcc ccatataacg gcgttcttcg
    68821 atatggatac gaattttttc gcctgggctt aagtattctg gtacctgaat caccagacca
    68881 gtgctcaatg tcgccggttt gttacgggcg ctggcagatg cccctttgat gcctggtgca
    68941 gtttcaacga tttccagatc aacggtctgc ggcagctcaa gcgccagcag ttgaccgtcc
    69001 caggtcagca cctgcatgtc cggcatgccg ccctctggca taaacagcaa ctcttcttca
    69061 atctgatctt tggtgaaggt atacggggta tagtcttctt tatccataaa gacatattcg
    69121 ttgccatcga cataggagaa gtcaacgtaa cggcgggtca gcgtcacggt gtcaacgata
    69181 tcatcacctt tgaaacgctc ttctactttc agcccggtac ggacatcaga aaaacgcatt
    69241 ttgtacagcg tagcggcacc gcgggcagtg ggcgactgaa tatcaatatc cttaaccagc
    69301 agcagtttgc cgttgtaatt cagtaccata ccttttttaa tttcgttcgc tcttggcatc
    69361 gaaaaattcc tgtagtcgaa aatgtcaaaa atatcgcgac aaagtactcg cgacgggcct
    69421 ttcgggcaag tggtattcgc acttttgctg gtgcaaaaaa ggtggtactg tgcgcgctca
    69481 tcaatccggt ggttaacctt aagagaacaa cgtatggaat gccgtccggg ttgcggggcg
    69541 tgttgcactg ccccttcaat ttccagccct attcccggta tgccagatgg caagcccgcc
    69601 aatacgccct gcattcagct tgatgaacag caacgctgta aaattttcac ctcgccactg
    69661 cgaccgaaag tctgtgcagg cttacaagcc agcgctgaaa tgtgcggtaa ctcgcgtcag
    69721 caggccatga cctggcttat cgatctggaa atgctgaccg cgccttaaac atctttaatc
    69781 cgcagtaagc aaaacagagt ggcgataatc atcaccatcg caaaccagaa cacagcgtga
    69841 taattccaga tctcggcgac gatgcccgcc actgatcctg cgatgatcca gcccacgcgc
    69901 gaagtgttgg tatagagcgt ggtggctgaa cccgcctgac cgggcatcag atcctgaaaa
    69961 tagagcatcc cgatgccgcc cagaatgcca ataaaaatag catttagcag ctgcaagccc
    70021 aacagaatga caggtgaatg cgccatcagc attcctgcgt aaaaacagac gccacccacg
    70081 gcagcaacgc gcattaagaa acgcttaccc agacgtttgg cgaaatatcc ggcaatcaac
    70141 atcgtcggga tttccagccc ggcggcggtc cccatcatca caccggccag tttctcggga
    70201 agatgcagtt cgttgataat aaatagcggc atgttgatga tgtacaggct gttcgagccc
    70261 cacatcaatg tacaaatgac aaacagcagt agcgtatcgc gacggttacg gcgcggtgct
    70321 tcaaccgtgc cggtcgccag cggaagctct ttttgcatcg acggtaaaaa cagccacacc
    70381 atcacgccgc aaacaataaa cgctaccgct gcgctcagat acattaccgt aaagctgaaa
    70441 cccatcgcta acgcataagc cagcggtggg ccaatgaccc atgccagtga aacctgagcg
    70501 cgtaaaaaag agctgaacat caccgcctca cgtccggttt tgtcggcatg ttcacgggca
    70561 agggcaaaca tttgcgggtt agcggtcgag ccaaagctgc taagaaagac gccaacgaat
    70621 agcaaaacaa agtagttgcg attccaggca aaaagggtgc aggccagcac gcctaacagg
    70681 cagcaaaaga caatcagcga tttgcgatcg ccgcgcttat cagagcgccc ggcgagaaac
    70741 tgacttacca gaatcccaat gacagcgctg ccggtaaaga agaatcccac catcgccgga
    70801 cgggcatgta cttcatcggt aagaaaaata ctgagtgtcg gggtttgcag agcacccgca
    70861 ataccggtga gaaaggcaac gattaaaaac gccgtcgagg tcaggtcaaa cgatttcgcg
    70921 ctggagagtg cgggggagtt atgcatgcag gtcgatcact ggaagaggaa gacgcggagt
    70981 ttacgctgct tgtcagaaaa taaacagaca gcgaaatcaa aagcgccacg aataatcaaa
    71041 atgccatttc actgttgctg attggctgct gatgctgctg aaatgaaatt gctgataacg
    71101 gattttccca tcagcaatta ggaaaaatga caaaaaattt tgcagcacat caaacttttg
    71161 ctcataactt tacggctttc cttgcgtgct gaaacagaag tattatgctt tattgaaacg
    71221 tttcagcgcg atcttgtctt taaccctaag ccaggttggc gctttttttc tcatagaggc
    71281 tgaatcgttt caattcagca agagaggaga actatgttcc agttatccgt acaggacatc
    71341 catccgggcg aaaaggccgg agacaaagaa gaggcgattc gccaggtcgc tgcggcgctg
    71401 gtgcaggccg gtaatgtagc agaaggctac gtcaatggca tgctggcgcg cgaacagcaa
    71461 acctcaacgt tcctcggcaa tggtattgct attccacacg gcactaccga cacccgcgat
    71521 caggtgctga aaaccggcgt tcaggtattt cagttcccgg aaggcgtcac ctggggtgac
    71581 ggtcaggtag cgtacgtggc aatcggtatt gctgccagct cggatgaaca tctgggcctg
    71641 ctacgccagc tgacccacgt actgagcgat gattccgttg ctgaacaact gaagtcagca
    71701 acaacagcag aagaacttcg cgcattactg atgggcgaaa agcagagtga gcagctgaag
    71761 ctcgacaacg aaatgctgac gctggatatc gtcgccagcg atctgctgac tcttcaggcg
    71821 ctgaacgctg cgcgtctgaa agaggcgggg gcagttgacg ccactttcgt caccaaagcc
    71881 atcaatgaac aaccgctgaa tctcggacag ggtatctggc tgagcgatag cgccgaaggc
    71941 aatctgcgta gcgcgattgc ggtaagccgt gcggcaaatg cttttgatgt ggacggcgaa
    72001 acggcagcca tgctggtgag tgtggcgatg aatgacgatc agcccatcgc ggttcttaag
    72061 cgtctcgctg atttattgct cgacaataaa gctgaccgct tgctgaaagc ggatgcggca
    72121 acgttgctgg cgctgctgac cagcgatgat gcgccgaccg acgacgtgtt aagcgcggag
    72181 tttgtggtgc gcaatgaaca cggcctgcat gctcgtccag gtaccatgct ggtcaatacc
    72241 attaaacaat ttaacagtga tattaccgtg acaaaccttg atggcaccgg caaaccggca
    72301 aacggacgta gtctgatgaa agttgtggca cttggcgtta agaaaggtca tcgcctacgc
    72361 tttaccgccc agggtgcaga tgctgaacag gcgctgaaag ccattggcga cgctatcgct
    72421 gctggtcttg gggagggcgc ataatgagca gacgtgttgc tacgatcacc cttaatccgg
    72481 cttatgacct tgttggtttc tgcccggaaa ttgaacgcgg cgaagtgaac ctggtgaaaa
    72541 ccaccggtct gcatgcggcg ggtaaaggca tcaacgtggc caaagtatta aaagacctgg
    72601 gaattgatgt caccgttggc ggcttcctgg gtaaagacaa tcaggatggt tttcagcaac
    72661 tgttcagcga gctgggcatt gccaaccgtt tccaggttgt acaggggcgc acccgaatta
    72721 acgttaagct gacggaaaaa gacggcgaag tgaccgactt caacttctcg ggttttgaag
    72781 tcacccccgc cgactgggaa cgctttgtga ctgattctct gagctggctc ggtcagttcg
    72841 atatggtctg tgtcagcgga agcttaccgt caggcgtcag cccggaagcg ttcaccgact
    72901 ggatgactcg cctgcgtagt cagtgtcctt gcattatctt tgatagtagc cgtgaagcgt
    72961 tagtagcagg tttgaaagcg gcaccgtggc tggtgaaacc taaccgccgc gagttggaaa
    73021 tctgggcagg ccgtaaactg cctgaaatga aagatgtgat tgaagctgca catgcgctac
    73081 gtgaacaagg tattgcgcat gttgttattt cactgggtgc cgaaggcgcg ctttgggtta
    73141 atgcctccgg cgaatggatc gccaaaccac cgtcagtcga tgtcgtaagc accgttggcg
    73201 caggggattc tatggttggt ggcctgattt atggcttgct gatgcgtgaa tccagtgaac
    73261 acacactgcg tctggcgaca gctgttgcag ccctggcggt aagtcaaagc aatgtgggta
    73321 ttaccgatcg tccgcagttg gccgcaatga tggcgcgcgt cgacttacaa ccttttaact
    73381 gacagcagga gaggcataat gaaaacgctg ctgattattg acgctaatct cggtcaggca
    73441 cgcgcctata tggcgaagac cctgctgggc gcggcggcgc gaaaagcaaa actggaaatc
    73501 atcgacaatc cgaacgacgc agaaatggcg attgttctcg gtgattccat cccgaatgac
    73561 agcgcgctga acggtaaaaa tgtctggctg ggtgatattt cccgggcagt tgcgcaccct
    73621 gagctgttcc tgagtgaagc caaaggccat gcgaaacctt acactgcgcc ggtcgctgcg
    73681 acagcaccgg ttgctgccag cggtccgaaa cgcgtagttg cggtgactgc ttgcccgact
    73741 ggcgtagcac acacctttat ggcggctgaa gccattgaaa ccgaagcgaa aaaacgtggc
    73801 tggtgggtga aagttgaaac ccgtggttct gttggcgcgg gcaatgcaat cacccctgaa
    73861 gaagtcgcag cagcggatct ggtgattgtg gcggcagata tcgaagtgga tctggcgaaa
    73921 tttgctggta aaccgatgta tcgcacctct accggtctgg cgctgaagaa aactgcgcag
    73981 gaactggata aagcggttgc tgaagcaacg ccgtatgaac cagcgggcaa agctcaaaca
    74041 gcgaccactg aaggtaagaa agagagtgca ggcgcttacc gtcacttgct gacgggcgtc
    74101 tcttacatgc tgccgatggt tgttgcaggt ggtctgtgta tcgcgctttc ttttgctttt
    74161 ggtatcgaag cgtttaaaga gccgggtacg ttggcagcgg cgctgatgca gattggcggt
    74221 ggttcagcct ttgcgctgat ggtgccggta ctggcaggtt atattgcctt ctccattgcc
    74281 gatcgtccgg gcctcacgcc ggggctgatt ggcggtatgc tggctgtcag caccggttct
    74341 ggcttcattg gcggtattat tgcgggcttc ctggctggtt acattgcgaa gttaatcagt
    74401 acgcaactga aactgccaca gagtatggag gcgctgaaac caatcctgat cattccgcta
    74461 atttccagtc tggtggtcgg tctggcgatg atctacctga tcggtaaacc ggttgctggc
    74521 attctcgaag ggctgactca ctggctgcag accatgggga ctgcgaatgc ggttctgctg
    74581 ggggcgatcc tcggtggcat gatgtgtact gacatgggcg gtccggtaaa caaagcagcg
    74641 tacgcattcg gtgtgggtct gctgagtact caaacctatg gcccgatggc ggcgattatg
    74701 gcggcaggta tggtgccacc gctggcaatg ggtctggcaa caatggtggc gcgtcgcaaa
    74761 ttcgacaaag cgcagcagga aggtggcaaa gccgctctgg tattgggact gtgcttcatt
    74821 tcggaaggtg caattccgtt tgctgctcgt gatccgatgc gtgtgctgcc gtgctgtatc
    74881 gtgggtggtg cgctgactgg cgcaatctca atggcgattg gtgcgaaact gatggcacca
    74941 cacggtggtc tgtttgttct gctgatccct ggcgctatta cgccggtact gggttacctg
    75001 gtagcaatta ttgccggtac gctggtggcg ggtttggcct atgccttcct gaaacgtccg
    75061 gaagtggacg cagtagcgaa agcagcgtaa taaaaggtgt gttacagggc agaaatttcc
    75121 tgccctgtat cattggatga gcgcaaaggg aatgaccagc ttgtgaatgt aattttatga
    75181 atttaaatgg ttttatcttt cagtagaaac ttgaaacgtt catcaaacac taaggttatt
    75241 tcctcaaggg cagattattt tctgcccttt tttattgttc ctgttctgtg catcccgtca
    75301 caaattcctc gcttttgtaa ttcaggcatt taatcgcatt ttgtgagcct gctcaaatct
    75361 gcccattttg cctctctggc ggtaatttac tttacgtaaa acatgcatac tatgagcaca
    75421 tgtttaaata tgaacacatg tttaaaccgt ctttgcagta tctatattct tgtggattga
    75481 cagggttggt aattttttac cagaggacgt gctatgcgcg aaaaggatta tgtcgtaatt
    75541 ataggctcgg cgaatattga tgtcgccgga tattcacatg aatcattaaa ttatgcggat
    75601 tcaaatccag gtaaaataaa atttacgcct ggtggagtcg ggcgcaatat tgcacaaaac
    75661 ctggcgttgc tggggaacaa agcctggcta ctgaccgccg taggcagtga cttttatggt
    75721 caatcgctgc taacgcaaac caatcaatct ggcgtttatg tcgataaatg cctgattgtg
    75781 ccaggagaaa atacgtcaag ctatttatca ttcctcgata ataccggtga aatgctggtt
    75841 gctataaatg acatgaatat tagcaacgct attacagctg aatatctcgc acagcaccgt
    75901 gaatttattc agagggcaaa ggtcattgtc gcagactgta atatcagtga agaggcactg
    75961 gcatggattc tggataatgc cgccaacgta cccgtatttg tcgatccggt ttcagcgtgg
    76021 aaatgtgtca aagtgcgcga ccgtctaaat cagatccaca ctctcaagcc aaaccgcctt
    76081 gaagcggaaa ccctgagtgg gattgcgctg tcagggcgtg acgatgtggc aaaagttgct
    76141 gcctggttcc atcaacatgg cctgaaccga ctggtattga gcatgggcgg cgacggcgtt
    76201 tattacagcg atatcagcgg tgaaaatggc tggtctgcgc cgatcaaaac caatgttatt
    76261 aatgttaccg gagcgggcga tgccatgatg gcgggacttg cttcgtgttg ggtagacgga
    76321 atgccgtttg ccgaatctgt tcgtttcgca cagggatgtt cgtcaatggc gctctcctgt
    76381 gaatacacca ataaccccga tttatcgatt gccaacgtta tatcgttagt ggagaacgca
    76441 gaatgtctga attaaaaatt tcccctgaat tattacaaat ttccccggaa gtgcaggacg
    76501 ctttaaaaaa caaaaaaccg gttgtggcgc tggaatcgac cattatttct cacgggatgc
    76561 cgttcccaca aaatgcccag accgcaattg aagtagaaga aactattcgt aaacagggcg
    76621 cagtacctgc cactatcgcc attattggcg gcgtgatgaa agtgggttta agcaaagaag
    76681 aaattgaatt actgggtcgt gaagggcata acgtgaccaa agttagtcgt cgcgatttac
    76741 cttttgttgt tgccgccgga aaaaatggcg caaccactgt ggcttcaacg atgattattg
    76801 cggcgcttgc cggaattaaa gtatttgcca ccgggggaat tggtggtgtg catcgcgggg
    76861 cggaacatac cttcgatatt tctgccgatt tgcaagaact ggcaaatact aatgtcaccg
    76921 ttgtttgtgc cggggcgaaa tctattctcg atttaggatt aacaacagaa tatttagaaa
    76981 ccttcggtgt gccgttaatt ggctatcaga caaaagcgct gcctgcgttt ttctgccgta
    77041 ccagcccatt tgaagtcagt attcgccttg atagtgccac ggaaatcgct cgtgcaatgg
    77101 cagtgaaatg gcaaaccggt ttgaacggcg gcctggtagt ggcgaatccg atcccggaac
    77161 agtttgcgat gccggaggaa tctatcaatg cagccataga tcaagccgtc gccgaagccg
    77221 aagagcaggg cgttattggt aaagaaagta cgccattcct gctggcgcgc gttgctgaac
    77281 tgaccggtgg tgacagcctg aaatccaata tccagctggt gttcaacaac gccattctgg
    77341 cgagtgaaat tgccaaagaa taccagcgtc tcgcgggtta atttctgacg ggcagaaagc
    77401 cgtcgcccgt actcatcaag cataccaaaa cctggcgctg acgccgggtt tactggcatg
    77461 aagggaaaat cattatggat ataatgagaa gtgttgtggg gatggtggtg ttactggcaa
    77521 tagcatttct gttgtcagtg aataaaaaga gcatcagttt gcgcacggtt ggagccgcac
    77581 tgctgctgca aatcgctatt ggtggcatca tgctctactt cccaccggga aagtgggcag
    77641 tagaacaggc ggcattaggc gttcataaag tgatgtctta cagtgatgcc ggtagcgcct
    77701 tcatttttgg ttcgctggtt gggccgaaaa tggatgtcct gtttgacggt gcgggtttta
    77761 tcttcgcctt tcgcgtactt ccggcgatta ttttcgttac tgcgctcatc agtctgctgt
    77821 actacattgg cgtgatgggg ctgctgattc gcatccttgg cagcattttc cagaaagctc
    77881 ttaacatcag caaaatcgaa tcttttgtcg cagtcacaac tattttcctc gggcaaaatg
    77941 agatcccggc gatcgttaaa ccgtttatcg atcgcatgaa tcgcaacgag ttgtttaccg
    78001 caatttgtag cgggatggcg tccattgctg gttcgatgat gattggttat gccggaatgg
    78061 gcgtaccaat tgactacctg ttagcggcat cgctgatggc gatcccaggt ggtattttgt
    78121 ttgcacgtat tcttagcccg gccaccgagc cttcgcaggt cacatttgaa aatctgtcgt
    78181 tcagcgaaac gccgccaaaa agctttatcg aagcggcggc gagcggtgcg atgaccgggc
    78241 taaaaatcgc cgctggtgtg gcgacggtgg taatggcgtt tgtcgcaatt attgcgctga
    78301 tcaacggcat tatcggcgga attggcggct ggtttggttt cgccaatgcc tctctggaaa
    78361 gtatttttgg ctatgtactg gcaccgctgg cgtggattat gggcgtggac tggagtgatg
    78421 ccaaccttgc gggtagcctg attgggcaga aactggcgat taacgaattc gtcgcttacc
    78481 tgagtttctc cccatacctg caaacgggcg gcacgctgga agtgaaaacc gtggcgatta
    78541 tatcctttgc gctttgtggt tttgctaact ttggttctat cggtgttgtc gttggcgcat
    78601 tttcggctat ttcgccaaaa cgtgctccag aaatcgccca gcttggttta cgggcgctgg
    78661 cagcagcaac gctttccaac ctgatgagtg cgactattgc cgggttcttt attggtctgg
    78721 cgtaatgctc gatttgtaga ccggataaaa cgtattaagc gttgcatccg gcaatcgcgc
    78781 tattgcaaat aatttttact gcatcatccc ggagaattta ttctccgggt ccatctccag
    78841 cgccagtcct gacaactgct ttctgttttt aatgagatac cctttcttgc ttttagttaa
    78901 taaaccgtcg tgaatgaatt gcgcgagaac atataaaaga tgtcgataag aaacgcctaa
    78961 atactctgcc gcttgcgtat gcttttcgtg ataaagatca ccttcctgcg agagtaatat
    79021 aaaagctgcc aggcgattaa ctaacggaaa tgattgattc tgagttaaag aaacaatatt
    79081 acgataattt ttatgactta aggtgacgca gagttttcgt aaaaatagcg tgtcgtttaa
    79141 taacagcggg cggtaatgtt tcatagagag cgcaaggcac caacactctt caatagcctg
    79201 caccgcacgc ggttcatggt ctttgtcgat taactcaatc tcaccaataa aacagggggc
    79261 ggcaaagaaa tcgatcagcg acacgcgacc attagcaagc gtggcgtaaa gcctggcgcg
    79321 gcctcgcgtc agataaaaca gccaggacgg ctgttgccct tcctgcacaa tgtagtcacg
    79381 cgctaaaaaa tgaaacagcc gcgtatcggc tgaaacatcc gttaaaaagc aatccttaaa
    79441 cgcggattca ctcatcaatt gctgcttaag atcattattg tggatttctt tcacgatgtt
    79501 tatccgctat gagatttctc atatttagca tacgtacgcg gttggtaaga ttcaatcatc
    79561 ggcccggtcg aattttcttt gctgtagaaa aaataacgca actggaaaca gaggaaataa
    79621 acaatggaaa agagaaaaat tattctggat tgtgatccgg gtcatgatga tgctattgca
    79681 atgatgatgg cagcgaaaca tccggcaata gatttattag gcatcactat tgtagcgggt
    79741 aatcagacgc ttgataaaac attaattaat ggcctgaatg tttgccagaa actggagatt
    79801 aatgttccgg tttatgcggg gatgccgcaa cccattatgc gtaaacaaat cgttgccgat
    79861 aatattcacg gtgaaaccgg actggatggc ccggtattcg agccgctgac ccgccaggca
    79921 gaaagtaccc acgcggtgaa atacatcatt gataccctga tggcaagcga tggcgatatc
    79981 actctggtgc cggttggtcc gctttcaaat atcgcggtgg caatgcgtat gcaacccgcg
    80041 atcctgccca aaatccgtga aattgtgcta atgggcggcg cttacggtac aggcaacttc
    80101 acgccatctg ccgagttcaa catctttgct gatccggaag cagcccgcgt ggtgttcacc
    80161 tccggcgttc cattagtgat gatgggcctc gatctcacca accagaccgt ttgcaccccg
    80221 gacgtgattg cacggatgga aagggcaggc ggccctgccg gagagctgtt cagcgacatc
    80281 atgaacttca ctctcaaaac gcagttcgaa aactacggcc tggctggcgg cccggtgcac
    80341 gacgccacct gcatcggtta tctgattaac cctgatggca ttaaaaccca ggagatgtac
    80401 gtcgaagtgg acgtcaacag tggcccttgc tatgggcgta ccgtctgcga cgagctgggc
    80461 gttcttggca agcccgctaa taccaaagtc ggcatcacta ttgataccga ctggttctgg
    80521 ggcttagtcg aagagtgcgt gcgcggctac atcaaaaccc attaagtgtt gataacaagc
    80581 cgggtcagta tcgcaagatc caagcccggc tatttctgat gggcagaaag ccgtcgccca
    80641 atatgacatg aagagataat aactatggat gtcatgagaa gtgttctggg aatggtggta
    80701 ttgctgacga ttgcgttttt gctgtcagta aacaagaaga agatcagcct gcgtaccgtt
    80761 ggcgcggcgt tagtgttaca ggtcgtgatt ggcgggatta tgctttggtt accgccaggg
    80821 cgttgggtcg ctgaaaaagt cgcttttggc gtgcataaag tgatggcgta cagcgacgcg
    80881 ggtagcgcat ttatcttcgg ttcgctggtc gggccgaaaa tggacacgct gtttgatggc
    80941 gcaggattta tctttggttt cagggtatta ccggcaatta tcttcgtcac tgcactggtg
    81001 agtattctct actacatcgg tgtgatgggg attttaattc gcattctcgg cggtatattc
    81061 cagaaagcat taaatatcag caagattgag tcattcgtcg cggtcaccac cattttcctc
    81121 gggcaaaacg aaattccggc gatcgtcaaa ccctttatcg atcgtctgaa tcgcaatgaa
    81181 ttatttacag cgatttgtag tggcatggcc tcgattgctg gttcgacaat gattggttac
    81241 gccgccctgg gcgtacctgt ggaatatttg ctggcggcat cgttaatggc gatcccaggc
    81301 gggatcttgt ttgcccgcct gttaagcccg gctacggaat cttcgcaggt ttcctttaat
    81361 aacctctctt tcaccgaaac accgccaaaa agcattattg aagccgctgc gacaggggca
    81421 atgaccgggc tgaaaatcgc cgccggtgta gcgacagttg ttatggcatt tgtcgccatc
    81481 attgcgttaa ttaatggtat tatcggcggc gttggcggct ggtttggttt tgcacatgcc
    81541 tcgctggagt ccattttagg ttacctgttg gccccattgg cgtgggtgat gggggttgac
    81601 tggagtgatg caaatcttgc cgggagtttg attgggcaga agctggcgat caatgaattt
    81661 gtcgcttatc tcaatttctc gccatatctg caaacgggtg gcactctgga tgctaaaacc
    81721 gtggcgatta tttctttcgc gttgtgcggt ttcgctaact ttggttctat cggggtggtg
    81781 gtgggggcgt tttctgcggt tgcgccacac cgtgcgccgg aaatcgccca acttggttta
    81841 cgcgcgctgg cggcggcgac actttctaac ctgatgagtg ctactattgc aggattcttt
    81901 attggtttag cgtagtgcgc aatttatgag ccggataaga tgcggtcaag cgtcgaatcc
    81961 ggcaacattg ttacaccatt ggcactaatg aaagcgcgtt atcggcagac agggtagggt
    82021 tgtttaagct gccgctggcg cgcgaaattg ccgcgcaggc taccgcaaaa cgggcgctgt
    82081 cgcggaaact gtatccttca agaaagctat ataccaggcc cgccataaaa ccatcgtcag
    82141 caccaaaact gtctactgtc gtatgcgctg gcgcggtcag caaaaattgt tctccatcct
    82201 tttcgctgca ataaactgac tcatcgggca aataaacaaa cagttgctga acaccttgct
    82261 gatgcaatgc attcactgcg gcattacggt cagcatcgct ggtgatcgcc tgtccccata
    82321 aaatttccag ctccggtaaa gtgggtttca gggtgtgaat atgcgccagc cagtgtttga
    82381 ttttgcccgc tttgaattct gaaacggtat cgacaaacac cgggatttca tcagcgaggg
    82441 taaagaccca ttccagcgcc tcggctgtca ggttgcaatc agccagtacc acgcccgcat
    82501 gacgaagtaa atcgcgcgac ccgttcagta gttgcggtgt caactgttcc agcagatggg
    82561 tatcgttaat cgccagcacg gtttgatcgt ctcgattggc aattgccaga tacgtcgatg
    82621 tgctttgacc atgcaaacga acgcagccgg agacattcac gccggcacgg cgcgtttctt
    82681 ccaggagcat ttcgccataa aagtcatcgc caatcactga aagcaaatgg acgtcacggc
    82741 ctaacagcgc cagattgtgg gcgatgttgc gtcccacgcc gcccgccgag caatgaattg
    82801 taccgggatg agaagccgct tgcgggtaac ggatatccgc catcccgcga atatccatat
    82861 tgattgtccc caccactacg cagtattcct gctcggtgag aatgtaacct ttgcctttaa
    82921 tccgcccttt gcgcattaaa tccataatat gcgccgcaac gcgcgaacga ctgatttgca
    82981 gcatgtccgc aatttcgttc tgctgaatca gcgggttacg ccgtaaaatt gcaaggatct
    83041 ccttttcccg gttattcatc ttcaggctac cgctttttca gtttgttgcg ctttcagcca
    83101 ggcgatctct tctgcccaga tatccgggtt gatggtttca aggatcagcg gaatgccgtc
    83161 gaaacggtcg tcttgcatga tccagcggaa cgcatcatga ccgatattgc cttcaccgag
    83221 gctatgatgg cggtcaacgc ggctgccaaa ggtacttttc gcatcgttaa ggtgcatccc
    83281 gcgcagatac ttaaagccga caatacgggc aaaatcagcg aacgtttttt cgcattcggc
    83341 tggagtacgc aaatcatacc cggcagcgaa agcatggcag gtatcaatgc agacgccgac
    83401 gcgggattta tcttccacgc cgtcgataat cgccgcgaga tgttcgaatt taaaccctaa
    83461 gttactgccc tgaccggcgg tgttttcaat caccgctgtt acaccttgag ttttatccag
    83521 cgcaatgttg atggattcgg caatacgcgc aaggcaatcc tcttctgaaa tctgcatcag
    83581 atggctgcca gggtggaagt tgagcaaaga aagccccagt tgttcgcaac gctgcatttc
    83641 atctataaag gcatcgcgcg atttttccag agcttcagtg accggatgtc cgaggttaat
    83701 cagatagctg tcgtggggaa gaatttgcgc cgatgtgtag tgatattttt cacaggccgc
    83761 tttgaattca tcgatggttt gcgtcgtgag cggtgcggca cgccactgac gttggttttt
    83821 ggtgaacaag gcaaacgcgg ttgcgtcgat ttcggcggcg cgaattgcgg catttgccag
    83881 accgccagca gcactaacgt gcgctccaat gtatttcatg cgaggactcc tgttaaaccc
    83941 gctggaggaa aacggtaatg atagcgggtt aacaggagca agatgtagtg gtttatgcga
    84001 tgacgctttg aatcacatag ttaatcgcac caccaccaac aatcagccag gcaaacagta
    84061 ccagtgccat cagcagaggt ttcgccccgg cttttttcag cgcgctgacg tgagtggtca
    84121 gacccagcgc cgccatcgcc attgccagca ggaaggtatc cagcgttacc agcatgttca
    84181 ccacgctctg cggtaacagg tggaacgagt taaagatggc aactacgatg aacaagatgg
    84241 caaaccacgg aatagtgatt ttgcttttct cgccgctgtt cgccccagac agctgtttaa
    84301 cacgcgccgc cagcaggatg aggaacggag ccagcatcat cacgcgcagc attttggaaa
    84361 taactgctgc gttttccgca tccgggctga tggcatgacc tgccgccacc acctgcgcca
    84421 cttcgtgcac agtagaacca atgtagatac cgaaagtttc cggactaaac cattgagaca
    84481 tcagcggata tatcgccggg tagaggaaaa tagcgacggt cccgaagata acaacggttg
    84541 caacagccac ggttacttta ctggcttccg ctttcactac tggctcagtc gccagtaccg
    84601 cggcagcacc acagatactg ctaccggcac cgatcaacca gctggtgtgc ttatccagac
    84661 caaacacttt ctgccccagg aagcaagcca gcaggaaggt actggacagc gtcagcacgt
    84721 caatgatgat cccactgata ccgacatcgg caatttgcga gaacgtcaga cggaagccat
    84781 aaagaatgat acccagacgt aataaatatt gcttggcaaa cagcacacca ccgtcacagc
    84841 ttttccagat gtgcggatag atggtgttgc ctaaaaccat ccccaacaag attgcgaggg
    84901 tgagggcact aaacccggca cccgcaaccg cgggaatgga accaccccac agggcgaccc
    84961 cggtgataac tgcactcagg gctaaccccg gaataaaatg ccacagtgta cgatgttgtt
    85021 tctgtaaggt gatattcgtc ataaccctct cctttaaccg ggcataaggt tacgactaac
    85081 tggtttaaaa ataaaattga ttatatattt ataattaatc tttataagtg gtaagcgact
    85141 atgcacatca ccctccggca gttggaagtt tttgcagaag tattgaaaag tggatcaacc
    85201 acccaggcgt cggtgatgct ggcgttgtcg caatcagcag tgagcgcagc cttgaccgac
    85261 ctggaagggc agcttggcgt gcaactgttt gatcgcgtgg ggaaaagact ggttgttaat
    85321 gaacacgggc ggctgctcta tccgcgtgcg ttggcactgc ttgaacaggc ggttgaaatc
    85381 gaacaactgt ttcgcgaaga caacggcgcg attcgtatct atgccagtag taccatcggt
    85441 aactacattc tgcctgcagt tatcgctcgt tatcgccatg attatccgca gttgccgatt
    85501 gaacttagcg ttgggaacag ccaggacgtg atgcaagcgg tgctggattt ccgcgttgat
    85561 attggtttta ttgaaggacc gtgccacagc actgaaatta tttctgaacc gtggctggaa
    85621 gacgagctgg tggttttcgc cgcgccgact tcgccgttgg cccgtggtcc ggtcacctta
    85681 gagcagctgg ccgctgcgcc gtggatcctg cgtgaacgcg gttccggcac gcgggagatt
    85741 gtcgattatc tattgctgtc acatttaccg aagtttgaga tggcgatgga attaggtaac
    85801 tccgaggcaa tcaaacatgc ggtgcgtcat gggttgggaa ttagttgcct gtcgcgacgg
    85861 gtgattgaag atcaattgca ggcaggcaca ttaagtgaag ttgcggtccc tctgccgcgc
    85921 ctgatgcgta cgttgtggcg tatacatcat cggcaaaaac acctttccaa cgcgctacgg
    85981 cgctttctgg actattgcga tcccgcgaat gtaccgcgtt aagttgctgt acaagaacat
    86041 gctggtgctg tgtcgatttc gtgacgcagc gccctcagca tgcattcgcc agaaaagaga
    86101 ttggctgctt tacttataat ccctgggcga tcatgaaggt gtcttataac cgtgtatttc
    86161 tgccggaagg attgccaatc gtctgctaca atcgcgcctc atttttaaga tggatagcat
    86221 ttttgtatgg tttccgaaac taaaaccaca gaagcgccgg gcttacgccg tgaattaaag
    86281 gcgcgtcacc tgacgatgat tgccattggc ggttccatcg gtacaggtct ttttgttgcc
    86341 tctggcgcaa cgatttctca ggcaggtccg ggcggggcat tgctctcgta tatgctgatt
    86401 ggcctgatgg tttacttcct gatgaccagt ctcggtgaac tggctgcata tatgccggtt
    86461 tccggttcgt ttgccactta cggtcagaac tatgttgaag aaggctttgg cttcgcgctg
    86521 ggctggaact actggtacaa ctgggcggtg actatcgccg ttgacctggt tgcagctcag
    86581 ctggtcatga gctggtggtt cccggataca ccgggctgga tctggagtgc gttgttcctc
    86641 ggcgttatct tcctgctgaa ctacatctca gttcgtggct ttggtgaagc ggaatactgg
    86701 ttctcactga tcaaagtcac gacagttatt gtctttatca tcgttggcgt gctgatgatt
    86761 atcggtatct tcaaaggcgc gcagcctgcg ggctggagca actggacaat cggcgaagcg
    86821 ccgtttgctg gtggttttgc ggcgatgatc ggcgtagcta tgattgtcgg cttctctttc
    86881 cagggaaccg agctgatcgg tattgctgca ggcgagtccg aagatccggc gaaaaacatt
    86941 ccacgcgcgg tacgtcaggt gttctggcga atcctgttgt tctatgtgtt cgcgatcctg
    87001 attatcagcc tgattattcc gtacaccgat ccgagcctgc tgcgtaacga tgttaaagac
    87061 atcagcgtta gtccgttcac cctggtgttc cagcacgcgg gtctgctctc tgcggcggcg
    87121 gtgatgaacg cagttattct gacggcggtg ctgtcagcgg gtaactccgg tatgtatgcg
    87181 tctactcgta tgctgtacac cctggcgtgt gacggtaaag cgccgcgcat tttcgctaaa
    87241 ctgtcgcgtg gtggcgtgcc gcgtaatgcg ctgtatgcga cgacggtgat tgccggtctg
    87301 tgcttcctga cctccatgtt tggcaaccag acggtatacc tgtggctgct gaacacctcc
    87361 gggatgacgg gttttatcgc ctggctgggg attgccatta gccactatcg cttccgtcgc
    87421 ggttacgtat tgcagggaca cgacattaac gatctgccgt accgttcagg tttcttccca
    87481 ctggggccga tcttcgcatt cattctgtgt ctgattatca ctttgggcca gaactacgaa
    87541 gcgttcctga aagataccat tgactggggc ggcgtagcgg caacgtatat tggtatcccg
    87601 ctgttcctga ttatttggtt cggctacaag ctgattaaag gaactcactt cgtacgttac
    87661 agcgaaatga agttcccgca gaacgataag aaataagttt cctcccttcc ttgctaagcc
    87721 ctctcaaccg agagggcttt ttcaattcca tttccctgac aaatcatgcg gatataaaat
    87781 ttaacatttg gattgataat tgttatcgtt tgcattatcg ttacgccgca atcaaaaaag
    87841 gctgacaaat cagaggctgt tccggctttc tgggatgatc acctgcataa aaaataagtc
    87901 caccgcgatg ctgccgtacg caaggggacg tgaagaagat gtgagcgata acccatttta
    87961 ttttcgtagt tacctcatgg agatatggaa tgtttaggtt gaaccctttc gtacgggtcg
    88021 ggctgtgttt gtccgctatt tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg
    88081 aaacgatggt tgtcactgca tcttccgtgg aacaaaatct taaagatgca cctgccagta
    88141 tcagcgtcat tacccaggaa gacctgcagc gaaaaccggt acagaatctg aaggatgtcc
    88201 tcaaagaagt gcctggcgta caactgacga acgaagggga taaccgtaag ggcgttagta
    88261 ttcgtggtct ggacagcagc tataccctga ttctcgtcga cggtaaacgc gtgaactccc
    88321 gcaatgccgt cttccgccac aatgatttcg atctgaactg gatcccggtc gattccatcg
    88381 aacgtattga agtggtccgt ggcccgatgt cgtcgctgta cggttccgat gcgctcggcg
    88441 gtgtagtgaa tatcatcacc aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg
    88501 ataccaccat tcaggaacat cgcgatcgcg gtgacaccta taacggtcag ttctttacca
    88561 gtggaccatt aattgatggt gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg
    88621 aaaaggatga cccgcaaaac tcaacgacca ccgataccgg agaaacgccg cgtattgaag
    88681 gattctccag ccgcgacggc aatgtcgaat ttgcctggac accgaatcaa aatcacgatt
    88741 ttactgccgg atacggtttc gaccgtcagg atcgtgattc cgactcgctg gacaaaaacc
    88801 gcctggaacg ccagaactac tccgtcagcc ataatgggcg ttgggattac ggcaccagcg
    88861 aactgaaata ctacggtgag aaagtcgaga acaaaaaccc tggcaacagc agcccgataa
    88921 cttccgaaag caatacggtc gacggcaaat acacgttgcc gctgacggcg attaatcagt
    88981 ttctcacggt tggcggtgaa tggcgtcacg acaaacttag cgatgcggtg aacctgaccg
    89041 ggggaaccag ctccaaaacg tctgccagcc agtacgcgct gtttgtggaa gatgaatggc
    89101 ggatcttcga gccgctggcg ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg
    89161 gtgaacactg gagtccgcgt gcctacctgg tttataacgc caccgacacc gtaacggtga
    89221 aagggggctg ggcgacggca tttaaagcac cttctctgtt gcaacttagc cctgactgga
    89281 cgagcaattc ctgccgtggc gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa
    89341 ccagcgaaag ttgggagctg gggctttact acatgggtga agaaggctgg ctggaagggg
    89401 ttgaatccag cgttaccgtt ttccgtaacg atgtgaaaga tcgcatcagc atcagccgta
    89461 cgtctgacgt caatgctgca ccgggctacc aaaactttgt cggttttgag acgggcgcta
    89521 acggacggcg cataccggta tttagctact acaacgttaa caaagctcgt attcagggcg
    89581 tggaaaccga actgaaaatt ccgttcaacg atgaatggaa actgtcattc aactacacct
    89641 acaacgatgg tcgtgatgtc agcaacggcg aaaacaaacc gctatccgat ctgccgttcc
    89701 ataccgctaa cggtacgctg gactggaaac cgctggcgct ggaagactgg tcattctatg
    89761 tttctggtca ctataccggg cagaaacgag ccgacagcgc gacggctaaa acacctggcg
    89821 gttataccat ctggaatacc ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg
    89881 caggcgtgct gaaccttggc gacaaggatc tcagccgtga tgactacagt tataacgaag
    89941 atggacgtcg ttactttatg gcagtggatt atcgcttctg atgagaagat gcccggcgaa
    90001 ccgggcggac tttcacttca gtaaatactg cgcatggaag cgcaggtgat cctctataaa
    90061 agaggcgatg aagtagtaac tgtgatcata tcccggctga atacgcagcg tcatcggcca
    90121 cgctttctgg cgggcggctt ctgccagaac tgcgggttgc aactggtcgg caagaaactg
    90181 atcattatcg ccctgatcga taagtgtcgg gattgcatcc tgcgcattgc tggcgtacat
    90241 caacgcacaa ctgtcccatt ccagccatgc atttttgtcc tcacctaaat aggtgctaaa
    90301 cgctttaatg ccccagggta cgctgcacgg attcacaatt ggcgcaaagg ccgaaacgct
    90361 agtgtattta cccggatttt tcagcgccat aatcagcgca ccgtgaccac ccattgagtg
    90421 accgctaatg gcgcagcggt cgctgacatt aaattgcgac tgaaccagcg ccggtaattc
    90481 atcgcgcaga taatcataca tccggtaatg cgtcgcccac ggcggttgcg tggcattaag
    90541 ataaaagcct gcgccctggc ctaaatcgta gccatcgtcg ttggcaacct tttcgccgcg
    90601 cgggctggtg tctggcatca ccagtacaat ccccagttcc gccgctaccc gctgggcacc
    90661 cgccttggtg gtgaatttct cgtcattgca ggttaatccg gaaagccagt acagcactgg
    90721 tggcggggtg tgatcacgag ggggtgggag aaagatactg aacgtcatcg ggcagtttaa
    90781 ggtactggag tcgtgtcgcc agcgttgctg ccagccttca aaacagcggt gctcttcgag
    90841 catttccatg cgtggctcct tgttgtgttg tttgcaattt cttcatcata ccgtttattc
    90901 aatgtgctgt gagtaacttt cacttccgta tttgcataac gatgttttaa catctgctga
    90961 tgaaaggcag cggcaattac aataattatc gctgtgaata ctggattatg tgcgccgcct
    91021 cacgcacaat aatcaggctg taaatcagct taataacttt gcccccacgc agggcggagg
    91081 cgtcacacct gcaggagaaa tcataaatgc catcactcag taaagaagcg gccctggttc
    91141 atgaagcgtt agttgcgcga ggactggaaa caccgctgcg cccgcccgtg catgaaatgg
    91201 ataacgaaac gcgcaaaagc cttattgctg gtcatatgac cgaaatcatg cagctgctga
    91261 atctcgacct ggctgatgac agtttgatgg aaacgccgca tcgcatcgct aaaatgtatg
    91321 tcgatgaaat tttctccggt ctggattacg ccaatttccc gaaaatcacc ctcattgaaa
    91381 acaaaatgaa ggtcgatgaa atggtcaccg tgcgcgatat cactctgacc agtacctgtg
    91441 aacaccattt tgttaccatc gatggcaaag cgacggtggc ttatatcccg aaagattcgg
    91501 tgatcggtct gtcaaaaatt aaccgtatcg tgcagttctt tgcccagcgt ccgcaggtgc
    91561 aggaacgtct gacgcagcaa attcttattg cgctacaaac gctgctgggc accaataacg
    91621 tggctgtctc gatcgacgcg gtgcattact gcgtgaaggc gcgtggcatc cgcgatgcaa
    91681 ccagtgccac gacaacgacc tctcttggtg gattgttcaa atccagtcag aatacgcgcc
    91741 acgagtttct gcgcgctgtg cgccatcaca actgattaaa aggcaggaac catggagcgc
    91801 aacgtcacgc tcgattttgt tcgcggcgtc gccattctgg ggatcctgct attaaacatc
    91861 agcgcctttg ggctaccaaa ggcggcttat ctcaatcccg cctggtacga tgctattacg
    91921 ccgcaggatg catggacctg ggcatttctc gatctcatcg gccaggtgaa attcctcacg
    91981 ctttttgcgc tgctgtttgg tgctggcctg caaatgttgc tgccccgtgg cagacgctgg
    92041 atccagtcgc ggttaacgct gttagtctta ttgggcttta ttcacggttt attgttctgg
    92101 gacggcgata ttctgctggc ttacgggctg gtgggcttaa tctgctggcg gctggtgcgc
    92161 gatgcgccat cggtaaaaag cctgtttaat accggcgtca tgctttatct ggtggggctt
    92221 ggcgttttgc tgttattggg gctgatttcc gacagccaga ccagccgcgc ctggacgccg
    92281 gatgcatcgg ctattttgta tgaaaaatac tggaagcttc acggcggcgt tgaagcgatc
    92341 agtaatcgtg ccgatggtgt tggcaacagt ttactggcac tgggcgcaca gtatggctgg
    92401 caactggcag ggatgatgct cattggtgcg gcgctgatgc gcagcggctg gctgaaaggg
    92461 cagttcagct tacgtcacta tcgtcgtact ggttttgtgc tggtggcgat tggggtgacc
    92521 attaaccttc ctgccatcgc cctgcaatgg cagctggact gggcgtatcg ctggtgcgcc
    92581 ttcttacttc agatgccgcg ggaactgagt gcgccgtttc aggcgattgg ctatgcgtcg
    92641 ctgttttatg gcttctggcc gcaattgagc cgctttaagc tggtgcttgc gatcgcctgc
    92701 gtcggacgga tggcgctgac caactatcta ttgcaaacgc tgatttgtac cacgcttttt
    92761 taccacctcg gtttgtttat gcattttgac cgcctggagc tgctggcgtt tgttattccg
    92821 gtatggctgg cgaatatcct cttctctgtt atctggctgc gttacttccg ccaggggccg
    92881 gtggaatggc tctggcgtca gttaactttg cgtgctgccg gaccggcaat atctaaaaca
    92941 tcaagataac gatctggatc acattcgtta acaaaacggc tgtaaccgtt tccattgctg
    93001 tgactcgatt cacgaagtcc tgtattcagt gctgacaaaa tagccgccag caagcagtca
    93061 tttactgcaa tctcataaca ggtagtgaat atgatcacca ttcgtgatgt agcgcgtcag
    93121 gctggcgtct ctgtggcaac ggtttcccgg gtgctcaata acagcacgct ggtcagtgcc
    93181 gacacgcgtg aagcagtaat gaaagccgtg agtgagctgg attatcggcc aaacgccaat
    93241 gcccaggcgc tggcaactca ggttagcgac accattggcg tggtggtgat ggacgtttct
    93301 gatgcgtttt tcggcgcgct ggtaaaagcg gtggatctgg tcgcccagca gcatcagaaa
    93361 tacgtgctaa tcggcaatag ctatcatgaa gcggaaaaag agcgtcacgc cattgaggtg
    93421 ttaattcgcc agcgttgtaa tgcgttgatt gttcactcaa aagcattgag tgacgatgaa
    93481 ctggcgcaat ttatggataa cattcccggt atggtgttaa tcaaccgcgt tgtgccgggg
    93541 tacgcccatc gttgcgtttg cctggataat ctcagcggtg cccgaatggc gacgcgcatg
    93601 ttgctgaata acggtcatca acgtattggt tatctttctt ccagccacgg cattgaagat
    93661 gacgccatgc gtaaagcagg ctggatgagt gcgttgaaag agcaggatat tattccgccg
    93721 gaaagctgga ttggcactgg tacgccggac atgccgggcg gtgaggcggc gatggttgaa
    93781 ctgctggggc gcaatctaca acttaccgct gtatttgctt ataacgacaa tatggccgct
    93841 ggctcactga cagcattaaa agataatggc attgcgattc cgttacatct ctcaatcatc
    93901 ggtttcgatg atattcccat cgcccgttac accgacccgc aattaacgac cgtgcgttat
    93961 cccattgctt caatggctaa attagccacc gaactggcct tgcagggggc agcaggcaat
    94021 attgatcctc gtgccagcca ctgttttatg ccgacgttag tgcgtcgcca ttctgtcgca
    94081 acgcgccaga atgcggcggc gatcactaac tcaacaaatc aggcgatgta accgctttca
    94141 atctgtgagt gatttcacag tatcttaaca atgtgatagc tatgattgca ccgttttaac
    94201 gttgtaaccc gtatgtaaca gtgaataatc actttcaccg aggtaacagc gtcataacaa
    94261 caattaaagc cgttttctgg agcgttaccg ggcatggaag aacgaatttt aaaaagtgag
    94321 cttcggcgtt cagtaacact tcattaactc tactgccccg ccgagcattt atctcaagca
    94381 ctaccctgca taagaaaaac cggagatacc atgaataaga aggtgttaac cctgtctgct
    94441 gtgatggcca gcatgttatt cggtgccgct gcacacgctg ctgatactcg cattggtgta
    94501 acaatctata agtacgacga taactttatg tctgtagtgc gcaaggctat tgagcaagat
    94561 gcgaaagccg cgccagatgt tcagctgctg atgaatgatt ctcagaatga ccagtccaag
    94621 cagaacgatc agatcgacgt attgctggcg aaaggggtga aggcactggc aatcaacctg
    94681 gttgacccgg cagctgcggg tacggtgatt gagaaagcgc gtgggcaaaa cgtgccggtg
    94741 gttttcttca acaaagaacc gtctcgtaag gcgctggata gctacgacaa agcctactac
    94801 gttggcactg attccaaaga gtccggcatt attcagggcg atttgattgc taaacactgg
    94861 gcggcgaatc agggttggga tctgaacaaa gacggtcaga ttcaattcgt attgctgaaa
    94921 ggtgaaccgg gccatccgga tgcagaagca cgtaccactt acgtgattaa agagctgaac
    94981 gacaaaggca ttaaaactga acagttacag ttagataccg ctatgtggga taccgctcag
    95041 gcgaaagata agatggacgc ctggctgtct ggcccgaacg ccaaaaaaat cgaagtggtt
    95101 atcgccaaca acgatgcgat ggcaatgggc gcggtagaag cactgaaagc acacaacaag
    95161 tccagcattc cggtgtttgg cgtcgatgcg ctgccagaag cgctggcgct ggtgaaatcc
    95221 ggtgcactgg cgggcacagt actgaacgat gctaacaacc aggcgaaagc gacctttgat
    95281 ctggcgaaaa acctggccga tggcaaaggt gcggctgatg gcaccaactg gaaaatcgac
    95341 aacaaagtgg tccgcgtacc ttatgttggc gtagataaat acaacctggc tgaattcagc
    95401 aagaaataag actgattggg cgcagtcgtt tactgcgccc ttattgtatg gccaacaagg
    95461 taaaattata tggtcagctc aacgactccg tcttccgggg aatacttgtt ggaaatgagc
    95521 ggtatcaaca agtcctttcc tggtgttaag gcacttgata acgttaattt aaaagtccgg
    95581 ccacattcta tccatgcatt aatgggggaa aacggtgcag gaaaatcgac attattaaaa
    95641 tgcctgtttg gtatttatca aaaagactcc ggcaccattt tattccaggg taaagagatc
    95701 gatttccatt ccgccaaaga ggcactagaa aatggtattt cgatggtaca ccaggagtta
    95761 aacctggtat tacaacgttc ggtgatggat aacatgtggc tggggcgata tcccaccaaa
    95821 ggcatgtttg tcgatcagga caaaatgtac cgcgaaacca aagcgatttt tgatgaactg
    95881 gatattgata tcgatccgcg tgcgcgcgtc ggcacattat ccgtttcgca aatgcagatg
    95941 atcgaaatcg ccaaagcgtt ttcctataac gcgaaaattg tgattatgga tgaaccgact
    96001 tcttcgttaa ccgaaaaaga ggtcaatcat ctgttcacta ttattcgtaa attaaaagag
    96061 cgcggctgcg gtattgttta tatctcgcat aaaatggaag aaatcttcca gttatgtgat
    96121 gaagttaccg tattgcgcga cggtcagtgg atcgccaccg aaccgctggc aggactgacg
    96181 atggacaaga tcatcgccat gatggttggg cgttctctta atcagcgttt tcctgacaaa
    96241 gaaaacaagc cgggcgaagt catcctcgag gtgcgtaacc taacgtcact gcgccagccg
    96301 tcgattcgcg atgtctcgtt tgatctgcat aaaggggaga tcctcggtat tgccgggctg
    96361 gtgggggcga aacgtaccga tattgttgag acgttatttg gtattcgcga gaaatcggct
    96421 ggcaccatta cgttgcacgg caaacagatc aataaccata atgccaacga agccataaac
    96481 cacggatttg cactggtaac tgaggagcgc cgctcaacgg gaatttatgc ctatctggat
    96541 attggtttta actcgttaat ttccaatatt cgcaactaca aaaataaagt tggtttactg
    96601 gataactcgc ggatgaaaag cgatacccag tgggtgattg attcgatgcg ggtaaaaacg
    96661 ccgggtcatc ggacgcaaat tggttcgctc tccggtggta atcagcaaaa ggtgattatt
    96721 ggtcgctggc tactaacgca accagaaata ttaatgctcg atgaaccgac gcgcggtatt
    96781 gatgtcgggg cgaagtttga aatttatcag ttaattgccg aactggcgaa gaaaggcaag
    96841 gggattatta ttatctcctc tgaaatgcct gagttgttag ggataacaga ccgtattctg
    96901 gtcatgagca atggtctcgt ttccggaatt gtcgatacaa aaacaacaac gcaaaacgaa
    96961 attctgcgtc ttgcgtcttt gcacctttaa gattaggggc ttcccatgag tgcgttaaat
    97021 aagaaaagtt ttcttactta cctgaaagag ggcggtattt acgtcgttct tttagttttg
    97081 ctggcgatta ttattttcca ggacccaaca tttttaagtc tgttgaactt aagtaatatt
    97141 ctcacccagt catcggtgcg tattattatc gcgctcggtg tggcagggtt aattgtcacc
    97201 caggggaccg atctttctgc tggtcgtcag gtagggctgg cggcagtggt ggctgcgaca
    97261 ttattgcagt ccatggataa cgccaacaaa gtgttcccgg aaatggcgac gatgccgatt
    97321 gcgctggtta ttctgattgt ctgtgccatt ggtgcggtga tcggtttgat caacggtctg
    97381 attatcgctt atctcaacgt gacgccgttc attaccacgc tcggcacgat gatcatcgtc
    97441 tatggcatca actcgctcta ttacgacttt gtcggggcgt cgccaatttc tggttttgac
    97501 agtggcttct ctacctttgc tcagggcttt gtcgcgctgg ggagtttccg tctctcttac
    97561 atcaccttct acgcgttgat tgcggtggcg ttcgtctggg tgttgtggaa caaaacccgc
    97621 ttcggtaaga acatttttgc cattggcggt aacccggaag cggcaaaagt atctggtgtc
    97681 aacgtcggcc tgaacctgct gatgatctac gcgttgtctg gcgtgttcta tgcctttggc
    97741 gggatgttag aagccggacg tatcggctct gccaccaaca acctcggctt tatgtatgag
    97801 ctggatgcta tcgcggcgtg cgtggtaggc ggtgtatcgt tcagcggcgg tgtggggacg
    97861 gtgattggcg tggtgaccgg ggtaattatt tttaccgtca tcaactatgg cctgacgtat
    97921 atcggcgtaa acccatactg gcagtacatc atcaaagggg cgattattat cttcgccgta
    97981 gcgctggatt cactgaaata cgcgcgtaag aagtgatgtg attgtggata ttgccggatg
    98041 cgctgcatcc ggcaatcggt gccaaatgcc ggatgcgtct ctggttttac aacgttaccg
    98101 ggtgttcttt ctcgcctttc ttaaacttca cttccccgag ctcaatacaa cccaccgggc
    98161 agacgtgacc acaaagcaga caacccacac atttctcggt attacaatgc ggtgtgcggg
    98221 ttttctcgct ccattccatc gcctggtgac cgccgtcgta acaggaaata taacagcgtc
    98281 cacagccaac acatttatca agattgatac ggggatagac aatataactg cggtctaaat
    98341 cttccgccgg gacaatattg ttattcgcca gacctaccat ctcctgcagc gaatcaaatc
    98401 cctgatcggc gagataatgc gacaacccgc ttgccatatc ttccactatc cgatacccgt
    98461 actgcatgat gccggtggtc acctgtaacg ttgctgcgcc gagcaataaa aactcagccg
    98521 catcctccca ggtttcaatg ccgccgatac cgctgattgg gaaatcgcgc agttctggat
    98581 gggtgcgcat ttgctgaatg aagcgcaggg cgatcggttt taccgcttta ccggaatatc
    98641 cggaaatact cgattttccg ttaacgatcg gcataccgat tttctgatta agatcgatat
    98701 tggtgatgga tttaacggtg ttaatcgccg caatgccatc tgcgccgccg cgcttcgccg
    98761 ccagcgccac ttcgcacata tcaccgatat tcggcgtcat cttcgccagc attggcagcg
    98821 tggaaccccg tttcactgcc cgacaatatt tttctaccag ctccgggctt tgcccgacat
    98881 cgctacccat cgcatgagaa gtcatttgcg gacaggagaa gttacactcg atcatatccg
    98941 cgccagcttc ttgcaccagg cgcgccagct cctcccattg ctgctcattt tcccccatga
    99001 tcgaagcgat caatactttg tccgggtaat cttccttcag ccgacgcagg gcggccagat
    99061 tctcttccaa cggatgttca gcaatctgct ccatattttt gaagccgata aaaccggtat
    99121 cttctttcac cagatgatca aaacgcggcg agacttcgtt ggcgataaaa aagccgatcg
    99181 ttttaaacac cacaccgccc caacctgtgt cgtaggcttt ggcacacatc tcatagcagt
    99241 tgcctaccgg cgaagaagag aggcagaacg ggttgggaaa cttcacgccg caaaaagtaa
    99301 tcgaaagatc tttcgttaac atgagcaagc tccctctaaa tagtgatgaa tcgcctcggc
    99361 ggcttctttc ccggttttca cggcatagac cacggttttg tcaccctcaa caatatcgcc
    99421 cgcagcaaag acttgcggat cgcgggtctg ataattttgt gtattgatag tgttacgctg
    99481 cggctctaac tccgcaaagg catccagtct ggcatgctga ccgacggcga gaataatttt
    99541 atctgccgcc atcgtcagtt cgcccggtaa ccgtacatgc ttaaacgtga ctttattgcc
    99601 ttccacggct actggcgtga atccatcgat gatcgaaacg cccagctctt gggcgctggt
    99661 aaactctttt tcgcttgccg gaaactcatc taactcttca cgcgctacgc aagttaccgc
    99721 ctgacagccg agaactttca gcgtgctggc tacgtccatc gcgacatcac cgccgccgat
    99781 aattaatgcg ctttgtggaa tgctgatatc gccttgcgcc tgtcgtacac gttgcaggaa
    99841 gtcgacggca atctcaacgt cgctatgctc aaacagcggt agcccggaac cgcttgataa
    99901 ccccacggtg accagtaccg cgcggttttc cgctttcagt tgctccaggg tgagtgtttt
    99961 accgatttcg ttattgcact taatggtcac gcccattttt tcaatacggg cgatctctgc
   100021 atccagcact gattgcggta accggaattg cggaataccg ttacgcaacc agccaccggg
   100081 gtgcgcttct ttctcataaa tcgtgacgtc ataaccctgg tttgtcagtg tcacactggc
   100141 ctgcaatccg gcaggacctg cgccaataat cgcgactttg ccgagcgttt tagtaccggg
   100201 ctgataaatt tccatcccgg tttgttgttc aaaatcagta acaaaacgct gtaagcggcc
   100261 gatatcaatg ggagcatcaa caccggcacg ggtacaaccg ctttgacata atttctccgt
   100321 cgggcacact ctggcacaaa cggcaccgag ggcattattt tcgcgaattg tctcggcagc
   100381 gcctttaaaa ttacgaaagt agattgagcg aataaatttc cccggatcgg tctgtgccgg
   100441 gcaagcctga ctacagggag cgtcatgaca taataaacag cgagaggctt ctttaatcgc
   100501 cagtaaaggc gtaaaagccg gagtgagttc atccagataa ttttgttgcg gcatgtgatc
   100561 ttccttaatg ctatgagtaa ttaaggcgaa cgctaaatat actcgtcata cttcaagttg
   100621 catgtgctgc gtctgcgttc gctcacccca gtcacttact tatgtaagct cctggggact
   100681 cactcacttg tcgccttcct gcaactcgaa ttatttagag tataaatatt gcgtgctgat
   100741 attaaggcta tgcgcgtggg gaaatttata ctgtgaaagg attcacaaat agctgcggtt
   100801 cagaatgtta tatccgcag
//
LOCUS       DAZKHC020000004        95614 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZKHC020000004 DAZKHC020000000
VERSION     DAZKHC020000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 95614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 95614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.004"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..239
                     /gene="ycgZ"
                     /locus_tag="ACHOSM_RS01580"
                     /old_locus_tag="pgaptmp_000316"
     CDS             3..239
                     /gene="ycgZ"
                     /locus_tag="ACHOSM_RS01580"
                     /old_locus_tag="pgaptmp_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HGU4556914.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            282..554
                     /gene="ymgA"
                     /locus_tag="ACHOSM_RS01585"
                     /old_locus_tag="pgaptmp_000317"
     CDS             282..554
                     /gene="ymgA"
                     /locus_tag="ACHOSM_RS01585"
                     /old_locus_tag="pgaptmp_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HGU4556915.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            583..848
                     /gene="ariR"
                     /locus_tag="ACHOSM_RS01590"
                     /old_locus_tag="pgaptmp_000318"
                     /gene_synonym="ymgB"
                     /pseudo
     CDS             583..848
                     /gene="ariR"
                     /locus_tag="ACHOSM_RS01590"
                     /old_locus_tag="pgaptmp_000318"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
     gene            961..1209
                     /gene="ymgC"
                     /locus_tag="ACHOSM_RS01595"
                     /old_locus_tag="pgaptmp_000319"
     CDS             961..1209
                     /gene="ymgC"
                     /locus_tag="ACHOSM_RS01595"
                     /old_locus_tag="pgaptmp_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HGU4556916.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            1541..3064
                     /gene="pdeG"
                     /locus_tag="ACHOSM_RS01600"
                     /old_locus_tag="pgaptmp_000320"
     CDS             1541..3064
                     /gene="pdeG"
                     /locus_tag="ACHOSM_RS01600"
                     /old_locus_tag="pgaptmp_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4556917.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            3195..3413
                     /gene="ymgF"
                     /locus_tag="ACHOSM_RS01605"
                     /old_locus_tag="pgaptmp_000321"
     CDS             3195..3413
                     /gene="ymgF"
                     /locus_tag="ACHOSM_RS01605"
                     /old_locus_tag="pgaptmp_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HGU4556918.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            3813..6461
                     /locus_tag="ACHOSM_RS01610"
                     /old_locus_tag="pgaptmp_000322"
     CDS             3813..6461
                     /locus_tag="ACHOSM_RS01610"
                     /old_locus_tag="pgaptmp_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECIAI39_1904 family autotransporter adhesin"
                     /protein_id="HGU4556919.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGEVK
                     DINNALVITTGNGSYGVSVAGVGSNLTINNGVIVTTGGLYPSDNSSLSTAYTASAVVS
                     EYGCAVTLRGNNTIVTTGDYSVGLLSQVDGNLNTDTIIRVNSDGSVTPSFSDGNNTFI
                     VTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGEITTHGTE
                     SYAAYANGTVVKADGTLDYTNASVTLTDVDITTHGDNAHAIATRQGTVSFNQGEIYTT
                     GPDAAIAKIYNGGTVTLKNTSAVAHQGAGVVLESSINGQEATVDILSGSSLRSANEIL
                     YNKNETSNVTITDSEVSSAADVFINNIKGHVTVDATDSKITGSANLSTDDNTHTYLSL
                     SDNSTWDIKADSTVSNLTVDNSTVYISRADGRDFEPTRLTITENYVGNNGVLHLRTEL
                     GDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEFIKDSRIF
                     AGAYEYSLTRGNTEATNKNWYLTNFQATNGGETNSGGSSAPTVAPTPVLRPEAGSYVA
                     NLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNGQLRTTSH
                     RYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRGYCAGLYA
                     TWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYGFALNKSF
                     GLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDTNNNIQTRLGFRTFIRTQEK
                     NSGPHDDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGARNLGEIKLGVNGNLNPAASV
                     WGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6518..6853)
                     /gene="ymgD"
                     /locus_tag="ACHOSM_RS01615"
                     /old_locus_tag="pgaptmp_000323"
     CDS             complement(6518..6853)
                     /gene="ymgD"
                     /locus_tag="ACHOSM_RS01615"
                     /old_locus_tag="pgaptmp_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HGU4556920.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(6857..7201)
                     /gene="ymgG"
                     /locus_tag="ACHOSM_RS01620"
                     /old_locus_tag="pgaptmp_000324"
     CDS             complement(6857..7201)
                     /gene="ymgG"
                     /locus_tag="ACHOSM_RS01620"
                     /old_locus_tag="pgaptmp_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HGU4556921.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGILKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(7203..7376)
                     /gene="ymgI"
                     /locus_tag="ACHOSM_RS01625"
                     /old_locus_tag="pgaptmp_000325"
     CDS             complement(7203..7376)
                     /gene="ymgI"
                     /locus_tag="ACHOSM_RS01625"
                     /old_locus_tag="pgaptmp_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HGU4556922.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7477..7662
                     /gene="ymgJ"
                     /locus_tag="ACHOSM_RS01630"
                     /old_locus_tag="pgaptmp_000326"
     CDS             7477..7662
                     /gene="ymgJ"
                     /locus_tag="ACHOSM_RS01630"
                     /old_locus_tag="pgaptmp_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HGU4556923.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            7623..7742
                     /locus_tag="ACHOSM_RS01635"
                     /old_locus_tag="pgaptmp_000327"
     CDS             7623..7742
                     /locus_tag="ACHOSM_RS01635"
                     /old_locus_tag="pgaptmp_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGU4556924.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            7777..8120
                     /locus_tag="ACHOSM_RS01640"
                     /old_locus_tag="pgaptmp_000328"
                     /pseudo
     CDS             7777..8120
                     /locus_tag="ACHOSM_RS01640"
                     /old_locus_tag="pgaptmp_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(8492..8758)
                     /gene="minE"
                     /locus_tag="ACHOSM_RS01645"
                     /old_locus_tag="pgaptmp_000329"
     CDS             complement(8492..8758)
                     /gene="minE"
                     /locus_tag="ACHOSM_RS01645"
                     /old_locus_tag="pgaptmp_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HGU4556925.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(8762..9574)
                     /gene="minD"
                     /locus_tag="ACHOSM_RS01650"
                     /old_locus_tag="pgaptmp_000330"
     CDS             complement(8762..9574)
                     /gene="minD"
                     /locus_tag="ACHOSM_RS01650"
                     /old_locus_tag="pgaptmp_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HGU4556926.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(9598..10293)
                     /gene="minC"
                     /locus_tag="ACHOSM_RS01655"
                     /old_locus_tag="pgaptmp_000331"
     CDS             complement(9598..10293)
                     /gene="minC"
                     /locus_tag="ACHOSM_RS01655"
                     /old_locus_tag="pgaptmp_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HGU4556927.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            10817..11185
                     /gene="ycgJ"
                     /locus_tag="ACHOSM_RS01660"
                     /old_locus_tag="pgaptmp_000332"
     CDS             10817..11185
                     /gene="ycgJ"
                     /locus_tag="ACHOSM_RS01660"
                     /old_locus_tag="pgaptmp_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HGU4556928.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            complement(11305..11706)
                     /gene="pliG"
                     /locus_tag="ACHOSM_RS01665"
                     /old_locus_tag="pgaptmp_000333"
     CDS             complement(11305..11706)
                     /gene="pliG"
                     /locus_tag="ACHOSM_RS01665"
                     /old_locus_tag="pgaptmp_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HGU4556929.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            11948..12241
                     /locus_tag="ACHOSM_RS01670"
                     /old_locus_tag="pgaptmp_000334"
     CDS             11948..12241
                     /locus_tag="ACHOSM_RS01670"
                     /old_locus_tag="pgaptmp_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HGU4556930.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            12313..12972
                     /gene="ycgM"
                     /locus_tag="ACHOSM_RS01675"
                     /old_locus_tag="pgaptmp_000335"
     CDS             12313..12972
                     /gene="ycgM"
                     /locus_tag="ACHOSM_RS01675"
                     /old_locus_tag="pgaptmp_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGU4556931.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLGVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            13064..13510
                     /gene="ycgN"
                     /locus_tag="ACHOSM_RS01680"
                     /old_locus_tag="pgaptmp_000336"
     CDS             13064..13510
                     /gene="ycgN"
                     /locus_tag="ACHOSM_RS01680"
                     /old_locus_tag="pgaptmp_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HGU4556932.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(13717..14639)
                     /gene="hlyE"
                     /locus_tag="ACHOSM_RS01685"
                     /old_locus_tag="pgaptmp_000337"
                     /pseudo
     CDS             complement(13717..14639)
                     /gene="hlyE"
                     /locus_tag="ACHOSM_RS01685"
                     /old_locus_tag="pgaptmp_000337"
                     /inference="COORDINATES: protein motif:HMM:NF017881.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            15006..15425
                     /gene="umuD"
                     /locus_tag="ACHOSM_RS01690"
                     /old_locus_tag="pgaptmp_000338"
     CDS             15006..15425
                     /gene="umuD"
                     /locus_tag="ACHOSM_RS01690"
                     /old_locus_tag="pgaptmp_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HGU4556933.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            15425..16693
                     /gene="umuC"
                     /locus_tag="ACHOSM_RS01695"
                     /old_locus_tag="pgaptmp_000339"
     CDS             15425..16693
                     /gene="umuC"
                     /locus_tag="ACHOSM_RS01695"
                     /old_locus_tag="pgaptmp_000339"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HGU4556934.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QTGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(16739..17269)
                     /gene="dsbB"
                     /locus_tag="ACHOSM_RS01700"
                     /old_locus_tag="pgaptmp_000340"
     CDS             complement(16739..17269)
                     /gene="dsbB"
                     /locus_tag="ACHOSM_RS01700"
                     /old_locus_tag="pgaptmp_000340"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HGU4556935.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(17415..18956)
                     /gene="nhaB"
                     /locus_tag="ACHOSM_RS01705"
                     /old_locus_tag="pgaptmp_000341"
     CDS             complement(17415..18956)
                     /gene="nhaB"
                     /locus_tag="ACHOSM_RS01705"
                     /old_locus_tag="pgaptmp_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HGU4556936.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            19178..19897
                     /gene="fadR"
                     /locus_tag="ACHOSM_RS01710"
                     /old_locus_tag="pgaptmp_000342"
     CDS             19178..19897
                     /gene="fadR"
                     /locus_tag="ACHOSM_RS01710"
                     /old_locus_tag="pgaptmp_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HGU4556937.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(19949..21481)
                     /gene="ycgB"
                     /locus_tag="ACHOSM_RS01715"
                     /old_locus_tag="pgaptmp_000343"
     CDS             complement(19949..21481)
                     /gene="ycgB"
                     /locus_tag="ACHOSM_RS01715"
                     /old_locus_tag="pgaptmp_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HGU4556938.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            21811..23109
                     /gene="dadA"
                     /locus_tag="ACHOSM_RS01720"
                     /old_locus_tag="pgaptmp_000344"
     CDS             21811..23109
                     /gene="dadA"
                     /locus_tag="ACHOSM_RS01720"
                     /old_locus_tag="pgaptmp_000344"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HGU4556939.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            23119..24189
                     /gene="dadX"
                     /locus_tag="ACHOSM_RS01725"
                     /old_locus_tag="pgaptmp_000345"
     CDS             23119..24189
                     /gene="dadX"
                     /locus_tag="ACHOSM_RS01725"
                     /old_locus_tag="pgaptmp_000345"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HGU4556940.1"
                     /translation="MPRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(24576..26312)
                     /gene="cvrA"
                     /locus_tag="ACHOSM_RS01730"
                     /old_locus_tag="pgaptmp_000346"
     CDS             complement(24576..26312)
                     /gene="cvrA"
                     /locus_tag="ACHOSM_RS01730"
                     /old_locus_tag="pgaptmp_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HGU4556941.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(26407..27321)
                     /gene="ldcA"
                     /locus_tag="ACHOSM_RS01735"
                     /old_locus_tag="pgaptmp_000347"
     CDS             complement(26407..27321)
                     /gene="ldcA"
                     /locus_tag="ACHOSM_RS01735"
                     /old_locus_tag="pgaptmp_000347"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HGU4556942.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            27421..28032
                     /gene="emtA"
                     /locus_tag="ACHOSM_RS01740"
                     /old_locus_tag="pgaptmp_000348"
     CDS             27421..28032
                     /gene="emtA"
                     /locus_tag="ACHOSM_RS01740"
                     /old_locus_tag="pgaptmp_000348"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HGU4556943.1"
                     /translation="MKLRWFAFLIALLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(28034..28768)
                     /gene="ycgR"
                     /locus_tag="ACHOSM_RS01745"
                     /old_locus_tag="pgaptmp_000349"
     CDS             complement(28034..28768)
                     /gene="ycgR"
                     /locus_tag="ACHOSM_RS01745"
                     /old_locus_tag="pgaptmp_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HGU4556944.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKLL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            28969..29223
                     /gene="ymgE"
                     /locus_tag="ACHOSM_RS01750"
                     /old_locus_tag="pgaptmp_000350"
     CDS             28969..29223
                     /gene="ymgE"
                     /locus_tag="ACHOSM_RS01750"
                     /old_locus_tag="pgaptmp_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGU4556945.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRGGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            29401..29841
                     /gene="ycgY"
                     /locus_tag="ACHOSM_RS01755"
                     /old_locus_tag="pgaptmp_000351"
     CDS             29401..29841
                     /gene="ycgY"
                     /locus_tag="ACHOSM_RS01755"
                     /old_locus_tag="pgaptmp_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HGU4556946.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(29920..31617)
                     /gene="treA"
                     /locus_tag="ACHOSM_RS01760"
                     /old_locus_tag="pgaptmp_000352"
     CDS             complement(29920..31617)
                     /gene="treA"
                     /locus_tag="ACHOSM_RS01760"
                     /old_locus_tag="pgaptmp_000352"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGU4556947.1"
                     /translation="MKSPAPSHPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNGQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(31937..33355)
                     /gene="dhaM"
                     /locus_tag="ACHOSM_RS01765"
                     /old_locus_tag="pgaptmp_000353"
     CDS             complement(31937..33355)
                     /gene="dhaM"
                     /locus_tag="ACHOSM_RS01765"
                     /old_locus_tag="pgaptmp_000353"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HGU4556948.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(33366..33998)
                     /gene="dhaL"
                     /locus_tag="ACHOSM_RS01770"
                     /old_locus_tag="pgaptmp_000354"
     CDS             complement(33366..33998)
                     /gene="dhaL"
                     /locus_tag="ACHOSM_RS01770"
                     /old_locus_tag="pgaptmp_000354"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HGU4556949.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(34009..35079)
                     /gene="dhaK"
                     /locus_tag="ACHOSM_RS01775"
                     /old_locus_tag="pgaptmp_000355"
     CDS             complement(34009..35079)
                     /gene="dhaK"
                     /locus_tag="ACHOSM_RS01775"
                     /old_locus_tag="pgaptmp_000355"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HGU4556950.1"
                     /translation="MKKLINDVRDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLVNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            35307..37226
                     /gene="dhaR"
                     /locus_tag="ACHOSM_RS01780"
                     /old_locus_tag="pgaptmp_000356"
     CDS             35307..37226
                     /gene="dhaR"
                     /locus_tag="ACHOSM_RS01780"
                     /old_locus_tag="pgaptmp_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HGU4556951.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(37326..40193)
                     /gene="ycgV"
                     /locus_tag="ACHOSM_RS01785"
                     /old_locus_tag="pgaptmp_000357"
     CDS             complement(37326..40193)
                     /gene="ycgV"
                     /locus_tag="ACHOSM_RS01785"
                     /old_locus_tag="pgaptmp_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4556952.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDAIINFTGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNVTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNNFREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(40961..42052)
                     /gene="ychF"
                     /locus_tag="ACHOSM_RS01790"
                     /old_locus_tag="pgaptmp_000358"
     CDS             complement(40961..42052)
                     /gene="ychF"
                     /locus_tag="ACHOSM_RS01790"
                     /old_locus_tag="pgaptmp_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGU4556953.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(42169..42753)
                     /gene="pth"
                     /locus_tag="ACHOSM_RS01795"
                     /old_locus_tag="pgaptmp_000359"
     CDS             complement(42169..42753)
                     /gene="pth"
                     /locus_tag="ACHOSM_RS01795"
                     /old_locus_tag="pgaptmp_000359"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGU4556954.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            43031..43309
                     /gene="ychH"
                     /locus_tag="ACHOSM_RS01800"
                     /old_locus_tag="pgaptmp_000360"
     CDS             43031..43309
                     /gene="ychH"
                     /locus_tag="ACHOSM_RS01800"
                     /old_locus_tag="pgaptmp_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HGU4556955.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(43364..45043)
                     /gene="dauA"
                     /locus_tag="ACHOSM_RS01805"
                     /old_locus_tag="pgaptmp_000361"
     CDS             complement(43364..45043)
                     /gene="dauA"
                     /locus_tag="ACHOSM_RS01805"
                     /old_locus_tag="pgaptmp_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HGU4556956.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(45168..46115)
                     /gene="prs"
                     /locus_tag="ACHOSM_RS01810"
                     /old_locus_tag="pgaptmp_000362"
     CDS             complement(45168..46115)
                     /gene="prs"
                     /locus_tag="ACHOSM_RS01810"
                     /old_locus_tag="pgaptmp_000362"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGU4556957.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(46266..47117)
                     /gene="ispE"
                     /locus_tag="ACHOSM_RS01815"
                     /old_locus_tag="pgaptmp_000363"
     CDS             complement(46266..47117)
                     /gene="ispE"
                     /locus_tag="ACHOSM_RS01815"
                     /old_locus_tag="pgaptmp_000363"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HGU4556958.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(47117..47740)
                     /gene="lolB"
                     /locus_tag="ACHOSM_RS01820"
                     /old_locus_tag="pgaptmp_000364"
     CDS             complement(47117..47740)
                     /gene="lolB"
                     /locus_tag="ACHOSM_RS01820"
                     /old_locus_tag="pgaptmp_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HGU4556959.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            47954..49210
                     /gene="hemA"
                     /locus_tag="ACHOSM_RS01825"
                     /old_locus_tag="pgaptmp_000365"
     CDS             47954..49210
                     /gene="hemA"
                     /locus_tag="ACHOSM_RS01825"
                     /old_locus_tag="pgaptmp_000365"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HGU4556960.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            49252..50334
                     /gene="prfA"
                     /locus_tag="ACHOSM_RS01830"
                     /old_locus_tag="pgaptmp_000366"
     CDS             49252..50334
                     /gene="prfA"
                     /locus_tag="ACHOSM_RS01830"
                     /old_locus_tag="pgaptmp_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGU4556961.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PVDLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            50334..51167
                     /gene="prmC"
                     /locus_tag="ACHOSM_RS01835"
                     /old_locus_tag="pgaptmp_000367"
     CDS             50334..51167
                     /gene="prmC"
                     /locus_tag="ACHOSM_RS01835"
                     /old_locus_tag="pgaptmp_000367"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU4556962.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            51164..51556
                     /gene="sirB2"
                     /locus_tag="ACHOSM_RS01840"
                     /old_locus_tag="pgaptmp_000368"
     CDS             51164..51556
                     /gene="sirB2"
                     /locus_tag="ACHOSM_RS01840"
                     /old_locus_tag="pgaptmp_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HGU4556963.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            51560..52369
                     /gene="sirB1"
                     /locus_tag="ACHOSM_RS01845"
                     /old_locus_tag="pgaptmp_000369"
     CDS             51560..52369
                     /gene="sirB1"
                     /locus_tag="ACHOSM_RS01845"
                     /old_locus_tag="pgaptmp_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HGU4556964.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            52405..53259
                     /gene="kdsA"
                     /locus_tag="ACHOSM_RS01850"
                     /old_locus_tag="pgaptmp_000370"
     CDS             52405..53259
                     /gene="kdsA"
                     /locus_tag="ACHOSM_RS01850"
                     /old_locus_tag="pgaptmp_000370"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HGU4556965.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(53408..53515)
                     /locus_tag="ACHOSM_RS01855"
                     /old_locus_tag="pgaptmp_000371"
     CDS             complement(53408..53515)
                     /locus_tag="ACHOSM_RS01855"
                     /old_locus_tag="pgaptmp_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4556966.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(53944..54051)
                     /locus_tag="ACHOSM_RS01860"
                     /old_locus_tag="pgaptmp_000372"
     CDS             complement(53944..54051)
                     /locus_tag="ACHOSM_RS01860"
                     /old_locus_tag="pgaptmp_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4556967.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(54456..55556)
                     /gene="chaA"
                     /locus_tag="ACHOSM_RS01865"
                     /old_locus_tag="pgaptmp_000373"
     CDS             complement(54456..55556)
                     /gene="chaA"
                     /locus_tag="ACHOSM_RS01865"
                     /old_locus_tag="pgaptmp_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HGU4556968.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     RSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            55826..56056
                     /gene="chaB"
                     /locus_tag="ACHOSM_RS01870"
                     /old_locus_tag="pgaptmp_000374"
     CDS             55826..56056
                     /gene="chaB"
                     /locus_tag="ACHOSM_RS01870"
                     /old_locus_tag="pgaptmp_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HGU4556969.1"
                     /translation="MPYKTKSDLPESVKHVLPSHALDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            56214..56909
                     /gene="chaC"
                     /locus_tag="ACHOSM_RS01875"
                     /old_locus_tag="pgaptmp_000375"
     CDS             56214..56909
                     /gene="chaC"
                     /locus_tag="ACHOSM_RS01875"
                     /old_locus_tag="pgaptmp_000375"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HGU4556970.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENFPDGVLRPGFA"
     gene            complement(56953..57306)
                     /gene="ychN"
                     /locus_tag="ACHOSM_RS01880"
                     /old_locus_tag="pgaptmp_000376"
     CDS             complement(56953..57306)
                     /gene="ychN"
                     /locus_tag="ACHOSM_RS01880"
                     /old_locus_tag="pgaptmp_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HGU4556971.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            57491..58885
                     /gene="ychO"
                     /locus_tag="ACHOSM_RS01885"
                     /old_locus_tag="pgaptmp_000377"
                     /gene_synonym="iatA"
     CDS             57491..58885
                     /gene="ychO"
                     /locus_tag="ACHOSM_RS01885"
                     /old_locus_tag="pgaptmp_000377"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HGU4556972.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(58886..59536)
                     /gene="narL"
                     /locus_tag="ACHOSM_RS01890"
                     /old_locus_tag="pgaptmp_000378"
     CDS             complement(58886..59536)
                     /gene="narL"
                     /locus_tag="ACHOSM_RS01890"
                     /old_locus_tag="pgaptmp_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HGU4556973.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(59529..61325)
                     /gene="narX"
                     /locus_tag="ACHOSM_RS01895"
                     /old_locus_tag="pgaptmp_000379"
     CDS             complement(59529..61325)
                     /gene="narX"
                     /locus_tag="ACHOSM_RS01895"
                     /old_locus_tag="pgaptmp_000379"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HGU4556974.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            61664..63055
                     /gene="narK"
                     /locus_tag="ACHOSM_RS01900"
                     /old_locus_tag="pgaptmp_000380"
     CDS             61664..63055
                     /gene="narK"
                     /locus_tag="ACHOSM_RS01900"
                     /old_locus_tag="pgaptmp_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HGU4556975.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            63571..67314
                     /gene="narG"
                     /locus_tag="ACHOSM_RS01905"
                     /old_locus_tag="pgaptmp_000381"
     CDS             63571..67314
                     /gene="narG"
                     /locus_tag="ACHOSM_RS01905"
                     /old_locus_tag="pgaptmp_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HGU4556976.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SFWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            67311..68849
                     /gene="narH"
                     /locus_tag="ACHOSM_RS01910"
                     /old_locus_tag="pgaptmp_000382"
     CDS             67311..68849
                     /gene="narH"
                     /locus_tag="ACHOSM_RS01910"
                     /old_locus_tag="pgaptmp_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU4556977.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAEAVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            68846..69556
                     /gene="narJ"
                     /locus_tag="ACHOSM_RS01915"
                     /old_locus_tag="pgaptmp_000383"
     CDS             68846..69556
                     /gene="narJ"
                     /locus_tag="ACHOSM_RS01915"
                     /old_locus_tag="pgaptmp_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU4556978.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            69556..70233
                     /gene="narI"
                     /locus_tag="ACHOSM_RS01920"
                     /old_locus_tag="pgaptmp_000384"
     CDS             69556..70233
                     /gene="narI"
                     /locus_tag="ACHOSM_RS01920"
                     /old_locus_tag="pgaptmp_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU4556979.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(70417..70548)
                     /locus_tag="ACHOSM_RS01925"
                     /old_locus_tag="pgaptmp_000385"
     ncRNA           complement(70417..70548)
                     /ncRNA_class="other"
                     /locus_tag="ACHOSM_RS01925"
                     /old_locus_tag="pgaptmp_000385"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(70596..70680)
                     /locus_tag="ACHOSM_RS01930"
                     /old_locus_tag="pgaptmp_000386"
     tRNA            complement(70596..70680)
                     /locus_tag="ACHOSM_RS01930"
                     /old_locus_tag="pgaptmp_000386"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70644..70646),aa:Tyr,seq:gta)
     gene            complement(70840..71682)
                     /gene="purU"
                     /locus_tag="ACHOSM_RS01935"
                     /old_locus_tag="pgaptmp_000387"
     CDS             complement(70840..71682)
                     /gene="purU"
                     /locus_tag="ACHOSM_RS01935"
                     /old_locus_tag="pgaptmp_000387"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HGU4556980.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(71732..72190)
                     /gene="ychJ"
                     /locus_tag="ACHOSM_RS01940"
                     /old_locus_tag="pgaptmp_000388"
     CDS             complement(71732..72190)
                     /gene="ychJ"
                     /locus_tag="ACHOSM_RS01940"
                     /old_locus_tag="pgaptmp_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HGU4556981.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            72303..73208
                     /gene="rssA"
                     /locus_tag="ACHOSM_RS01945"
                     /old_locus_tag="pgaptmp_000389"
     CDS             72303..73208
                     /gene="rssA"
                     /locus_tag="ACHOSM_RS01945"
                     /old_locus_tag="pgaptmp_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HGU4556982.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            73300..74313
                     /gene="rssB"
                     /locus_tag="ACHOSM_RS01950"
                     /old_locus_tag="pgaptmp_000390"
     CDS             73300..74313
                     /gene="rssB"
                     /locus_tag="ACHOSM_RS01950"
                     /old_locus_tag="pgaptmp_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HGU4556983.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            74515..75423
                     /gene="galU"
                     /locus_tag="ACHOSM_RS01955"
                     /old_locus_tag="pgaptmp_000391"
     CDS             74515..75423
                     /gene="galU"
                     /locus_tag="ACHOSM_RS01955"
                     /old_locus_tag="pgaptmp_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGU4556984.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(75567..75980)
                     /gene="hns"
                     /locus_tag="ACHOSM_RS01960"
                     /old_locus_tag="pgaptmp_000392"
     CDS             complement(75567..75980)
                     /gene="hns"
                     /locus_tag="ACHOSM_RS01960"
                     /old_locus_tag="pgaptmp_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HGU4556985.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            76585..77202
                     /gene="tdk"
                     /locus_tag="ACHOSM_RS01965"
                     /old_locus_tag="pgaptmp_000393"
     CDS             76585..77202
                     /gene="tdk"
                     /locus_tag="ACHOSM_RS01965"
                     /old_locus_tag="pgaptmp_000393"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGU4556986.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(77504..80179)
                     /gene="adhE"
                     /locus_tag="ACHOSM_RS01970"
                     /old_locus_tag="pgaptmp_000394"
                     /gene_synonym="adhC"
     CDS             complement(77504..80179)
                     /gene="adhE"
                     /locus_tag="ACHOSM_RS01970"
                     /old_locus_tag="pgaptmp_000394"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGU4556987.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            80572..80625
                     /gene="ychE"
                     /locus_tag="ACHOSM_RS01975"
                     /old_locus_tag="pgaptmp_000395"
     CDS             80572..80625
                     /gene="ychE"
                     /locus_tag="ACHOSM_RS01975"
                     /old_locus_tag="pgaptmp_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HGU4556988.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            80656..81303
                     /gene="ychE"
                     /locus_tag="ACHOSM_RS01980"
                     /old_locus_tag="pgaptmp_000396"
     CDS             80656..81303
                     /gene="ychE"
                     /locus_tag="ACHOSM_RS01980"
                     /old_locus_tag="pgaptmp_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HGU4556989.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(81461..81757)
                     /locus_tag="ACHOSM_RS01985"
                     /old_locus_tag="pgaptmp_000397"
     CDS             complement(81461..81757)
                     /locus_tag="ACHOSM_RS01985"
                     /old_locus_tag="pgaptmp_000397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4556990.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            82041..83672
                     /gene="oppA"
                     /locus_tag="ACHOSM_RS01990"
                     /old_locus_tag="pgaptmp_000398"
     CDS             82041..83672
                     /gene="oppA"
                     /locus_tag="ACHOSM_RS01990"
                     /old_locus_tag="pgaptmp_000398"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HGU4556991.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            83758..84678
                     /gene="oppB"
                     /locus_tag="ACHOSM_RS01995"
                     /old_locus_tag="pgaptmp_000399"
     CDS             83758..84678
                     /gene="oppB"
                     /locus_tag="ACHOSM_RS01995"
                     /old_locus_tag="pgaptmp_000399"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HGU4556992.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            84693..85601
                     /gene="oppC"
                     /locus_tag="ACHOSM_RS02000"
                     /old_locus_tag="pgaptmp_000400"
     CDS             84693..85601
                     /gene="oppC"
                     /locus_tag="ACHOSM_RS02000"
                     /old_locus_tag="pgaptmp_000400"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGU4556993.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            85613..86626
                     /gene="oppD"
                     /locus_tag="ACHOSM_RS02005"
                     /old_locus_tag="pgaptmp_000401"
     CDS             85613..86626
                     /gene="oppD"
                     /locus_tag="ACHOSM_RS02005"
                     /old_locus_tag="pgaptmp_000401"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HGU4556994.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            86623..87627
                     /gene="oppF"
                     /locus_tag="ACHOSM_RS02010"
                     /old_locus_tag="pgaptmp_000402"
     CDS             86623..87627
                     /gene="oppF"
                     /locus_tag="ACHOSM_RS02010"
                     /old_locus_tag="pgaptmp_000402"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HGU4556995.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(87680..88009)
                     /gene="yciU"
                     /locus_tag="ACHOSM_RS02015"
                     /old_locus_tag="pgaptmp_000403"
     CDS             complement(87680..88009)
                     /gene="yciU"
                     /locus_tag="ACHOSM_RS02015"
                     /old_locus_tag="pgaptmp_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HGU4556996.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(88044..89504)
                     /gene="cls"
                     /locus_tag="ACHOSM_RS02020"
                     /old_locus_tag="pgaptmp_000404"
     CDS             complement(88044..89504)
                     /gene="cls"
                     /locus_tag="ACHOSM_RS02020"
                     /old_locus_tag="pgaptmp_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGU4556997.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPKLMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            89647..89820
                     /gene="yciY"
                     /locus_tag="ACHOSM_RS02025"
                     /old_locus_tag="pgaptmp_000405"
     CDS             89647..89820
                     /gene="yciY"
                     /locus_tag="ACHOSM_RS02025"
                     /old_locus_tag="pgaptmp_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HGU4556998.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(89875..91128)
                     /gene="kch"
                     /locus_tag="ACHOSM_RS02030"
                     /old_locus_tag="pgaptmp_000406"
     CDS             complement(89875..91128)
                     /gene="kch"
                     /locus_tag="ACHOSM_RS02030"
                     /old_locus_tag="pgaptmp_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HGU4556999.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFITIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(91428..91724)
                     /gene="yciI"
                     /locus_tag="ACHOSM_RS02035"
                     /old_locus_tag="pgaptmp_000407"
     CDS             complement(91428..91724)
                     /gene="yciI"
                     /locus_tag="ACHOSM_RS02035"
                     /old_locus_tag="pgaptmp_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HGU4557000.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            91948..92667
                     /gene="tonB"
                     /locus_tag="ACHOSM_RS02040"
                     /old_locus_tag="pgaptmp_000408"
     CDS             91948..92667
                     /gene="tonB"
                     /locus_tag="ACHOSM_RS02040"
                     /old_locus_tag="pgaptmp_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HGU4557001.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(92707..93105)
                     /gene="yciA"
                     /locus_tag="ACHOSM_RS02045"
                     /old_locus_tag="pgaptmp_000409"
     CDS             complement(92707..93105)
                     /gene="yciA"
                     /locus_tag="ACHOSM_RS02045"
                     /old_locus_tag="pgaptmp_000409"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HGU4557002.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(93210..93749)
                     /gene="yciB"
                     /locus_tag="ACHOSM_RS02050"
                     /old_locus_tag="pgaptmp_000410"
     CDS             complement(93210..93749)
                     /gene="yciB"
                     /locus_tag="ACHOSM_RS02050"
                     /old_locus_tag="pgaptmp_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HGU4557003.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(93779..94522)
                     /gene="yciC"
                     /locus_tag="ACHOSM_RS02055"
                     /old_locus_tag="pgaptmp_000411"
     CDS             complement(93779..94522)
                     /gene="yciC"
                     /locus_tag="ACHOSM_RS02055"
                     /old_locus_tag="pgaptmp_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HGU4557004.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(94550..94639)
                     /locus_tag="ACHOSM_RS02060"
                     /old_locus_tag="pgaptmp_000412"
                     /pseudo
     CDS             complement(94550..94639)
                     /locus_tag="ACHOSM_RS02060"
                     /old_locus_tag="pgaptmp_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            94879..95517
                     /gene="ompW"
                     /locus_tag="ACHOSM_RS02065"
                     /old_locus_tag="pgaptmp_000413"
     CDS             94879..95517
                     /gene="ompW"
                     /locus_tag="ACHOSM_RS02065"
                     /old_locus_tag="pgaptmp_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HGU4557005.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
BASE COUNT        24309 a        22853 c        24051 g        24401 t
ORIGIN      
        1 gcatgcatca aaattcagtg acttcagatt ctgcgggagc aatcactcgt tactttgcaa
       61 aagctaactt gcctactcag caggaaactc tcggggaaat tgtgactgag attttgaaag
      121 atggacgtaa tctgagtcga aagtcgcttt gtgccaaact tctttgccga ctggaacaag
      181 cgacagggga agaggaacag aaacattata atgcactaat tgggttgctt tttgaataat
      241 gcaacagcct gattatttaa caggttagct atgaagtcgt tatgaagaca tctgataatg
      301 aacgtataaa atatgaaatt actggccagg cggtgctcca gatactgcgt atgaagataa
      361 atttttcatt gcagactctt attaagcaat tactcgtaat gaaatcggct gaggaagatg
      421 ctttccgacg cgatttaatt gacagcataa ttcgtgattt tagcaatagt gattcaggag
      481 ggccaaaccg aagaacagcg acagctgaca ataaaagtat gttcaatggt aagaaaataa
      541 acagaataca ttaaaatttc ataagtaaga tgagaggtta ccatgcttga agatactaca
      601 attcataatg caataactga taaagcgtta gcaagttact ttcgcagttc gggtaatttg
      661 ttagaagaag agtcagcagt gttagggcag gctgtcacca atttaatgct ttcaggcgat
      721 aatgttaata ataaaaatat tatcttaagt ctgatacacc cctggaaaca acaagtgata
      781 ttctcaaagc tgatgtgatt agaaaaacac tggaaatcgt gttgcgatac acagctgatg
      841 atatgtaact tctaattatt aagtataagt ttatagaaaa ctcattcatc ttatttttgt
      901 ctgtcgcttt agactataca cagcataatt ttattaggtt aatatttcta cgaggctgac
      961 atgaataatt caatcccaga gagatttatt tttcaatgtg ctttgtttaa aaatctcgag
     1021 agggaggtgt tcatgacgca tgggtatgtt gatagtcata ttattgatca ggctttacgt
     1081 ttgcgtttaa aagatgaaac cagtgtgatt ctttctgatc tctatctgca aatattgcag
     1141 tacattgaaa tgcataagac tacactaacg gatatcatta ttaatgacag ggaatccgtg
     1201 ctctcttaga acttgagtaa ggttttagaa taaacatcag ctgtatcacc atgctgatac
     1261 aaagtgagca tttcaggcgt tatgctttct tattacgtcc gcaatatcag gtgtcaagaa
     1321 tggagagttc tcgctctcca ttcttgacgc ctgatatccc gcctaactta ttttgtactg
     1381 cctacattat gctcaataat taatgggtta gatcaagatg gagaaaattt tatcttcggc
     1441 gtggatattt cttatgctct ctaccatgct ctctgtagat gccagcgcga gcgaatgaat
     1501 ggcattttca tgtattaaac cctttggccg ggacaagcaa atgcgcaata cactcatacc
     1561 catcctcgtt gcgatatgct tgtttattac cggtgtcgct attttgaaca tccagctctg
     1621 gtattccgca aaagcagagt acctggcggg agcaagatat gccgccaaca atatcaatca
     1681 tatacttgaa gaagcgtcac aggcgactca aacagcggtt aacattgccg ggaaggaatg
     1741 cgacctcgag gagcaatatc agcttggcac tgaagcagct ctgaaacctc acctgcgcac
     1801 aatcatcatt ctcaaacagg gaatagtctg gtgtacatcc ctgcctggga atcgggtcct
     1861 gctgtctcgt attcctgttg tcccggacag taatttactg ttggctccgg caatcgacac
     1921 cgttaataga ttacctatcc tgctctatca gagccaattt gcagatacgc gcattttggt
     1981 tacgataagt gatcaacata ttcgcggggc gcttaatgta cccttgaaag gggtaaggta
     2041 tgtattacgc gtggcggatg acattattgg accaacgggt gatgtgatga cgcttaatgg
     2101 acattatccc tataccgaga aggttcactc cacaaaatat catttcacta ttatctttaa
     2161 cccgccgcca ctctttagct tctacagact tatcgataaa ggctttggga ttttaatatt
     2221 tattctgtta atcgcctgcg ccgctgcctt cctgcttgat agatatttca ataaaagcgc
     2281 aacgcctgaa gagatcctgc gacgggcaat aaataatggg gagatcgtcc ctttttacca
     2341 acctgtggta aatggtcggg aagggacatt gcggggagtt gaggtgttag cccgctggaa
     2401 acaacctcac ggtggatata tatcacccgc ggcatttatt ccacttgctg aaaaatccgg
     2461 attaatcgtt ccgcttacgc aaagcctgat gaatcaggtt gccagacaga tgaacgctat
     2521 cgcgagtaaa ttgccggaag gttttcatat tggaattaat tttagcgctt cgcatattat
     2581 ttcgccgacg tttgtcgacg agtgtttaaa tttccgtgac agttttaccc gccgcgattt
     2641 aaaccttgtt ctggaagtca ccgagcgtga gccattgaat gttgatgaaa gtctggttca
     2701 gcggttgaac attctgcatg aaaatggttt tgtcatcgcg ctggatgatt tcggtactgg
     2761 ctactcaggg ctttcttatc ttcatgacct gcatattgat tatatcaaaa ttgatcacag
     2821 tttcgttggc cgcgtaaacg cagacccaga atcaacccga attctggatt gtgtattgga
     2881 tctggcgcgt aaactttcga tcagtatcgt cgctgaaggt gtcgaaacga aagaacaact
     2941 tgactatctg aaccaaaata atatcacatt tcagcagggt tattatttct ataaacctgt
     3001 tacatacatc gacctggtca aaattatcct ttctaaaccg aaggtgaagg ttgtagttga
     3061 gtgaaaattg atcagcaagg ccatagtgcg gtgtaattat agacagctaa ttagctcgtt
     3121 gcctcttgtt actttgttca ttattttgtt tgctataatt gtttgaaagt tttgacagga
     3181 ttgccattag tagcatgaac aatagtaata atctggatta tttcactctc tatatcatct
     3241 tttccattgc atttatgctg atcaccctcc tggtcatcct tattgcaaaa cccagtaccg
     3301 ggctgggaga agtgcttgtg acgataaatt tgcttaatgc ccttgtttgg ctggcgatca
     3361 atctggttaa tcgattaaga gaaagactcg tcaaccacag ggatcagcaa taatctttca
     3421 gtttctcact gtcagtatgc ggctgaatgg gttgctggca gtgaacgcct ggatcattga
     3481 aggaaaggca ttattgcgca aatagttgtc aaccctggtg ttatcacggt tgtttttata
     3541 tatcaccgaa ataatcctca tcgcaactat taacaatttt gatgtcgaag agttatttgt
     3601 taaacaaaat cgtcacccta aagtgatcaa tgtcatgaaa ataaggtgaa aaatgataat
     3661 gccgacttat ttatcattta tatattgtcg ctgtttatct atttaaattt tttgtgcttt
     3721 tgtttttatc tttaccctcc gttttggtaa tctgaacgca taggttattt gaccaggaat
     3781 gtttgttaat ccaatgaatg gatggttaat ctatgaaact gaaaaaactc tccgggttta
     3841 gtttgggact tattgctctg gcggtgggta atgcatatgc tgaacagttt attcagcacg
     3901 atgaatataa acatgctgtg gcaataaaag atggagaagt aaaagatatt aataatgcgc
     3961 tagttataac aacgggtaat ggaagttacg gggtatctgt tgcaggagtg ggtagcaatt
     4021 taaccattaa caatggcgtc attgtaacaa caggtggttt atatccttct gacaatagtt
     4081 ctctaagcac agcatataca gctagcgcag tcgtaagtga atatggctgt gctgtcactt
     4141 tgcggggtaa taatacgatt gtcacgacgg gagattattc tgttggtttg ttaagtcaag
     4201 ttgatggaaa cctgaatact gataccataa taagagtcaa ctctgacggt tcggttaccc
     4261 cctctttttc tgacggaaat aatactttta ttgttactgc gggtaatcac gcagtcggcg
     4321 ttcttgcgtg tgcatctccc ggaagcgcgc gtgcgtgtgt atcttctctt gatgaagaaa
     4381 gtactgccga tacaggaagt aatgaaaata acgccatagc aaaactggat atggcaaaag
     4441 gggagataac aacccacgga acggaaagtt atgccgctta cgctaacggc actgtcgtca
     4501 aggctgatgg cacgcttgat tataccaatg ccagcgttac attaaccgat gttgatatca
     4561 ccacgcatgg ggacaatgct catgccatcg ctactcgcca ggggacagtt tcatttaacc
     4621 agggagaaat ttatacaacg ggtcctgacg ccgcgattgc taaaatttat aatggcggca
     4681 cggtgacgct gaaaaataca tctgcagtcg cgcatcaggg ggctggagtt gtgctggagt
     4741 cttccataaa tggtcaggaa gcaacggtag atattttatc tggtagctca ctacggtcag
     4801 caaatgaaat cctttacaat aaaaatgaga cgagtaacgt gaccattact gatagtgagg
     4861 tatcatcggc tgcagatgtt tttatcaata atattaaagg tcatgtgacc gtcgatgcaa
     4921 ctgattcaaa aataacgggt tcagccaatc tttcaacaga tgataacact catacctatc
     4981 tgtcgctttc agataatagc acctgggata ttaaagctga ctcaacggtg agcaacctca
     5041 cggttgataa tagtacggtc tatatttccc gggcagatgg aagggatttc gaaccaacaa
     5101 gattaacaat aactgaaaat tacgttggta ataatggcgt attgcatctc agaactgaat
     5161 tgggtgatga taattcagct acggataaag tcgttattaa tggaaatacc tctggaacaa
     5221 ctcgagttaa agttaccaat gctggcggca gcggggctta cacgttaaat gggatagaga
     5281 ttatcagcgt tgagggggaa tcaaatggag aatttattaa ggattcgagg attttcgccg
     5341 gtgcctacga atattcatta acccgaggta ataccgaagc gaccaataaa aactggtatc
     5401 tgactaactt ccaggcaacg aacggcggtg aaacaaactc cggaggaagt tcagcgccta
     5461 ctgttgcgcc tacccccgtc ctgcgccccg aagctggaag ttacgtcgcc aacctggcag
     5521 ccgctaacac tctttttgtt atgcgtctga acgaccgtgc gggtgaaatg cgctacatcg
     5581 atcctgtaac tgaacaggag cgttcaagcc gactttggct acgtcaaatt ggcgggcata
     5641 atgcctggcg tgacagcaac ggacagttga gaacgacctc gcatcgctac gtctcgcagt
     5701 taggggccga tctgttaacc ggtggtttta ccgacagtga cagttggcgt ttgggagtga
     5761 tggctggtta tgcccgcgac tacaactcaa ctcattccag cgtgtcggat tatcgttcga
     5821 aagggagtgt cagaggctat tgcgcagggc tgtatgccac ctggtttgcc gatgacatca
     5881 gtaaaaaagg cgcatacatt gacgcctggg cgcaatatag ctggtttaaa aactcggtga
     5941 aaggggatga attagcctat gaatcctata gtgcgaaagg cgcaaccgtc tcgctggaag
     6001 cgggttacgg ctttgccctg aataaatcct ttggtctgga agcggcgaaa tacacgtgga
     6061 tattccagcc acaggcacag gctatctgga tgggcgtcga tcataatgcg cacaccgaag
     6121 ccaatggctc acgtattgag aatgacacaa ataacaacat ccagactcga cttggtttcc
     6181 gcacctttat tcgtactcag gagaaaaaca gcggtccgca cgatgacgac tttgaacctt
     6241 ttgttgaaat gaactggatc cataacagta aagattttgc tgtctcaatg aacggtgtga
     6301 aagtcgaaca agacggggcg cgtaatctgg gggaaattaa acttggcgta aatggcaatc
     6361 tgaatccggc ggccagtgtc tggggtaatg tgggcgtgca gctgggtgat aatggctaca
     6421 atgacaccgc agtgatggtg ggcctgaaat ataagttctg atcccgccgt tagctaaaaa
     6481 accgcgtcgt attcatcgac gcggtacata tgaaatatta ttttttgccg atagcacgca
     6541 tggtgtcatc aattgccgtg atcaacagca tttgcggatc tttagcgcaa acctgattca
     6601 gtttttctac cactttggcg ctcagttccg gagattgctc aatttttaaa tcacggctgg
     6661 caacgctggc attacccatt accgcaacaa tttctgcaac ctgtgcgctg tcagtttttg
     6721 ccatttcgtt ggcttctgcg caagtaatat aggtttctga cggcaaaccg tttttaatat
     6781 tgtagtcctg cgcccaggtc attggtgcga aaacaaacag gcccgccagt aaagcaaatt
     6841 ttttcatcat cattccttat ttcattttac ccagaattgc accacccgta ccgccaatca
     6901 cggcaccttt aatcgcccct tcgaggccat tgccggtcag aacgccagtg acagcaccaa
     6961 cggcggcacc cactttggca cctttacgcg catttttacc gtcgcggcct ttttctgtta
     7021 ctgcaccaac accagcgcca acagctgcac ctttcagtat gccattaaca ccattgccag
     7081 taagtaaacc aacgcctgcg cctagcaatg cacctttcgt ggtgcggttc atatccgcca
     7141 tcgctggcgt ggagcagaac aatgctgaga taagcccgaa ggcaagtatt tttttcttca
     7201 acttagatgt ccggtattaa gtaagttgca cacacaataa tttcgtcttc aattaagatc
     7261 tgcttaacta aagaacgctc gctattattc agataattca aaatgagcgt ggctgtgatg
     7321 ataggaatta tgttttttac gtgaatgaga ataatcttaa atgaggaata actcattgat
     7381 tggcaatatt tttattcaag aagtgtcatt gactgttaac gcaatgttgt aaaggtaaga
     7441 taatctgatt tatcaatatt attgtgtgat ttttatgtga gcagaagata ttcatcagca
     7501 acgattacat tagtcatttt attttgccga cggcctcatt gtcgaaagat aagcgtacga
     7561 cagtattatc tgaaaagagt gattttttat ccaactacac ttcagcgcac tgcgtttaaa
     7621 aaatgcctct ttcttatgcg ggatatcatc atttcatcat gatgtctttg gtgagcggtg
     7681 aacacaatac acctgcgctg tctcttcagg atgaatcccc tggtctggtg cctgcgggct
     7741 gatgttgcag cagagcttag gtcacttaga cgctactatc atttatccaa tggcatggaa
     7801 tcgaaatcag tcgatacccg cagtatatat cgtgaactgg gtgcaacgct gagttacaac
     7861 atgcgtctgg ggaacggtat ggaaattgag ccgtggctga aggcggctgt gcgcaaagaa
     7921 tttgtcgatg ataaccgggt gaaggtgaat agtgacggta atttcgtcaa tgatttgtcg
     7981 ggcagacgtg gaatatacca ggcaggtatt aaagcctcat tcagcagtac gttaagcggc
     8041 atctcggggt ggggtatagc catggtgccg gtgtggaatc cccgtggaat gcggtggctg
     8101 gtgtgaactg gtcgttctga ccatcaacga ttaaactgcg cttcggcgca gttttcgttt
     8161 acaggatgtt aaaagggaaa attctggggc aaaaaaagcc cgccagttac ggcgggaaac
     8221 ctcatcctat gggagaacaa tgaataatga aattgcgggg ttatcatctc ccagtatatc
     8281 cacgctaaca atgaggttat ttactcaacc aggcataaac attttgttct gtgcgtggga
     8341 acagccttaa ggtgtaaagg gggaggtgga aatagcaatg aggagtatca gcaagaatac
     8401 tcgccgcttt accacaacgt ggatgagagg gatgaaaaac tcaaggcaga aatgactctg
     8461 ccttgaagat aaatgcgttt ttacagcggg cttatttcag ctcttctgct tccggtaagg
     8521 tcacgttcag ctcaagaata gaaatatcgc catctttttg ctcaagctgt acggttacca
     8581 tctcaggatc aatttgtacg tatttacaaa tgacctcaag aatatcttta cgcaactgcg
     8641 gcagataatg cggttctgca tcgctgcgac ggcgttcagc aacaataatc tgcagccgtt
     8701 cttttgcaat gttggctgtg tttttcttcc gcgagagaaa gaaatcgagt aatgccataa
     8761 cttatcctcc gaacaagcgt ttgaggaagc ctttcttctc ttcttcaatg aagcggaaag
     8821 gacgttcttc tcccaacaga cgttctacgg tatctgcgta ggctttaccc gcatcggcgt
     8881 taatgtcgag aatgaccggt tcaccctggt tagaggcgcg caatactgat tgatcctctg
     8941 ggatcacgcc gacgagtttg atgcgcagga tctccagcac atcttccatg ctcagcatgt
     9001 cacctctgct tacgcggcct gggttatagc gcgttaacag caggtgctct ttaataggct
     9061 cttcgccatt ttctgcgcgg cgtgatttcg acgccagaat gcctaaaata cggtcagagt
     9121 cgcgtactga ggagacttcc gggttggtgg taataatggc ttcgtctgca aaatagagtg
     9181 ccattaacgc accggtttca atccctgccg gggagtcaca aacgataaat tcaaaatcca
     9241 tcgctttcag atcatcaaga actttggcga ccccttcacg ggtgagggca tctttatcgc
     9301 gtgtttgcga tgccggaaga atatagagat tttcagtacg cttatcttta attaacgcct
     9361 gatttagcgt tgcatcgccc tgaatgacgt tgacgaaatc gtaaacgacc cggcgttcac
     9421 aacccataat caggtcgaga ttacgcaggc cgatatcaaa atctatcacg acagttttct
     9481 ttcccttctg ggccaaacca gtggcgatgg ccgcgctgga ggttgtctta ccaacacccc
     9541 ctttgcccga agtaacaaca ataatgcgtg ccatagaaat tccttgttaa aaagggatca
     9601 atttaacggt tgaacggtca aagcgttttc gactaactgc agtcgcgccg ctttgccata
     9661 aaattctgct gggatttgat cactcagcca gtattcacct gcgatggaca ccagttccgc
     9721 catcaggttc gtacaaaata tttgcgtttc ccggtcacca cttgcccctg caagcgcacg
     9781 accgcgcatc atgccataga catgaatgtt cccatcggca atcaattcgg ccccagcgct
     9841 aacgtggctt gtaacaatca gatcacattg tggagcataa atacgctgac cggaacgcac
     9901 cggggtatct attaaacgcg tttttgtgac cggcgttgta ttttgcgctg gagcctgtgg
     9961 tgcgggagct ggacgcggcg ctttttcctt tccttccgtc aggataggca gccccatctt
    10021 ttcaatttcg gctttaagtt gcgcatcttt gcagccactt acgccaataa cccgcaaacc
    10081 ggttgccgaa accgccttat gcatcgctga ccagtttacc gggtcttcca gtgcactgac
    10141 gttgagtaca acgggggcat gttttaaaaa tgcgggggcc tgagcgattt tgtcttccag
    10201 cgcctgatgg ataaccttag gttctgcctc atgcagatga accacagata aagtgaagct
    10261 actgccttta agctcgattg gcgtgtttga catcctggcc ttactcaatt agctattaat
    10321 catcgccagc gcgcgatgat gttccgaaga ctataaggca tgttatagtc tggattatat
    10381 tgaggcaagt caccctccca tttattcaga gtaaaagtct attctgtgat aaatggcgct
    10441 gattcatagc ttaaaaaata cccttgtcaa tcaacccatt gccgccgtac ttttgattct
    10501 tcttatttac gcttcctttt tccgcaccct aatcgtgaca gcatctccag acctgtttaa
    10561 atatccagta ttgaggcaag tgtatttttt caccacgcca ggctttctta actttccatt
    10621 ttttgcagca tcattaaaga acagataccc gttatcttaa tatccgccac agaattgctc
    10681 tatcatagaa accgcagaag acgtctgata cttctgtcag gtcatcgtaa ctcgtccagg
    10741 tcagcggttt tgattacgga ttgatgacga aatgagcata agtgttgtac ggttggtgga
    10801 tttatcagga tgtgctatga atatgatgcg tattatttat atcggattgt caggtgtggg
    10861 gatgatgttc tcatcaatgg cgtctggaca tgacgctggc ggacttcaat ctccggcgtg
    10921 cggcgttgtt tgcgacccgt atatttgtgt gaactcagat ggcatttccc cagagttaac
    10981 aaggaaatat ctcggcgaaa aagccgctga aaacttacaa tcattacaag gctacgatcc
    11041 cagcgaattt acattcgcta acggtgtatt ttgcgatgtt aaagaaaaat tatgtcgtga
    11101 tgatcgttat tttggtgtgg atggtaagcg gagtggaaaa atcaatcaaa tcaccacaaa
    11161 gatgttattt atgtgtcgtg agtgatgcct tgtttacatt atgttctatt cggcattgtc
    11221 tgctattcgt cattaacctc ttgagcgaca gagaggagtg gggcgttaac gtttatgacg
    11281 gggagagtcc ccgtcggttg acatttactt aatttgaata tcgacgttat attttttagt
    11341 tttgttttta cgggcgtcgt ttcgggtttg cagcactcgc agctcatatt tgccagaagc
    11401 agggagtgag tattgtccgt gactatcgag ttctggggaa tatctggata aatcaacaga
    11461 atcatcgatt cctggcccga agaggtaagt atcagcgcct tcattagaga tactcacatg
    11521 cactttctga ccttttttgg catagaaggt atatgtatcg taatcgtatc cctttatttc
    11581 gccggagtac tgagcgctgc tatggccttt gctgaactca acattgacat tcttacccgc
    11641 agcaaagctc gtggaggtta gcagtgcaag cagcaataca gccttactga tgctcttgat
    11701 tttcattggt agtctcctgt tcagaatgag tacgatgaac tgttataata taacaatccc
    11761 ttacgggaga attccagtgg cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg
    11821 ccggggggat tacttataaa ggggcatgga ctcaacaaac cgcagaaatc tacagctggc
    11881 tggcttgtgg cgcgggtttc attgatgtta gcttatgcca aaacctggca tcttaaagag
    11941 taaaagtatg ttttgtgtga tttatcgaag cagcaagcgt gaccagacct atttatatgt
    12001 cgaaaaaaaa gacgattttt cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc
    12061 tcagttagcg atgattctgc cgttggatgg gcggaagaaa ctggtcaatg ctgatattga
    12121 aaaggttaaa ctggcattaa ccgagcaagg ttactatttg cagttaccgc caccacccga
    12181 agatttgctg aagcaacatc tttccgtcat ggggcagaaa acagacgaca ctaacaaata
    12241 accgatatcc ggcggtggca ttatctttgt cggcgcgggt tttcatatcc acgataaggt
    12301 gaggggaacg ttatgtatca acatcacaac tggcaaggtg cgctgctgga ttatccggtg
    12361 agtaaagtag tctgtgttgg cagtaactat gccaaacata ttaaagagat gggcagcgca
    12421 gtgcccgaag agccagtgct gtttattaaa ccagaaacgg cactgtgcga tctgcggcag
    12481 ccattggcga tcccatccga tttcggttca gttcatcatg aagtcgaact ggcggtgttg
    12541 attggcgcga cgctgcgtca ggcaacggaa gagcatgtcc gcaaagccat tgccggttac
    12601 ggcgtggcgc ttgatctgac attgcgtgat gtgcagggaa aaatgaagaa agccgggcag
    12661 ccgtgggaaa aggctaaagc gtttgataac tcttgtccgc tttccgggtt tatccccgcg
    12721 gcggaattta ccggcgatcc acaaaataca acgctgggcc tgggcgtaaa cggcgaacaa
    12781 cgccagcaag gtacgactgc ggacatgatc cataaaatcg ttccgttgat cgcttatatg
    12841 agcaagtttt ttaccctcaa ggccggtgac gttgtgttga caggcacgcc tgatggcgtc
    12901 ggcccgttgc aaagcggtga tgagctgaca gtcactttcg atgggcattc tttgacaact
    12961 cgcgttttgt aatacttttt gccgcctgaa agcggcggca aaacttgcat cgctgtgcca
    13021 gactggttat aaggtgcgtt ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt
    13081 ctggcaaagt aaaaccctgg acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg
    13141 ttgcggtcag tgttgcctgc ataaactgat ggatgaagac accgacgaaa tctacttcac
    13201 taacgtcgcc tgtcgccagc tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg
    13261 tttcgagttt gaacccgact gcattaaatt aacccgtgaa aatctgccaa cattcgaatg
    13321 gctgccaatg acctgcgctt atcggttgct ggcggaaggt aaagatttac ctgcgtggca
    13381 tccgctactt actggttcga aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat
    13441 cgcagtgaaa gaatcagaag tgattgactg gcaggatcat atcttaaata aacctgactg
    13501 ggcgcagtga taaatttaat agatgctgat gggggctttc ttaatatacg tctggatgct
    13561 cgccatttcg tagccctgtt tttgaaagcg ctctgtcata atgagagtta cccgatacct
    13621 tattatcaac aggtaactct caatctttaa aaaacgtctg tgtttttaaa tgtgataaac
    13681 cttatacctt gagtacatgg agagaataat cgcttatcag acttcaggta cctcaaagag
    13741 tgtcttttta ccgtgtcttt tctgatactc attacaggtg ttaatcattt ttttggccgc
    13801 ttcttttagc aaagaaagca ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt
    13861 ttccgttttt atctccccga tggcggctat ttcggtggtt aatttcaatt tggctgcatc
    13921 gatatcttta ttcgcttgtt taaccgtgtt agacagggtg gtaaagaaac tctgcacaga
    13981 ttttaacttg ttcttcaatt ctggaatcag tttcccttca actacgcccg cagcaataga
    14041 ataggaaatg attaaaccaa atggaccggc gacgacaccg gctgcggcac cggcatacgc
    14101 ttccttcctg attttatcta cctgtgactg gaaatagctg cttttttctg aaaaatcatt
    14161 ggttaactgg ctatctaacg ccagcagttt cccggaagcg ttgttgaaac tttgcgagct
    14221 taccagcagg gatttttgcg cttcattcag cttcgtgatg ccgtcatcca gtaccttaat
    14281 gagaatgtct ttctgggcgg atgctttctt ctcattgtac tcatcaaata gcaaaatata
    14341 cgcagcgagc aattgcgtcg caacaccaca ccacaccatt catacactgt ttgggttgct
    14401 tcaaaatact tatcctggct atccataagt aaggttttaa tatcgccgac taaaacggag
    14461 gctgcctgtg aatactcctg tttaaagcga cttaactctt ttatggtttc atcaaaggtc
    14521 tgccagggga tgacctgatc gagatattta ttataaagat ttaatgctcc atctgcggtt
    14581 tcgattgcgt ttttaactac ttctaccgtt ttatctgcaa cgatttcagt cataatcatt
    14641 cgcctcttta aatatataaa ttgtaatgaa actcctgttt tacaactatt aataaatttt
    14701 acttcatcta attcatagtt agccgggcgg gatgcgtcaa tgtctttatt tctattaata
    14761 tgataaatat caaacaatgt ttaatgtcat tatggcgaat gcttctattc tatttttttg
    14821 ccgggtgata tttttcattt ctgctggatg agcgtcgtcg ccagaaggcc acgtgagcac
    14881 aggataagag aacgaaaaat cagcagtcta tgcagcgaca aatattgata gcctgaatca
    14941 gtattgatct gctggcaaga acagaccact gtatataaaa acagtataac ttcaggcaga
    15001 ttattatgtt gtttatcaag cctgcggatc tccgcgaaat tgtgactttt ccgctattta
    15061 gcgatcttgt tcagtgtggc tttccttcac cggcagcaga ttacgttgaa cagcgcatcg
    15121 atctgaatca actgttgatc cagcatccca gcgcgactta cttcgtcaaa gcaagtggtg
    15181 attctatgat tgatggtgga attagtgacg gtgatttact gattgtcgat agcgctatta
    15241 ccgccagcca tggtgatatt gtcatcgctg ctgttgacgg cgagtttacg gtgaaaaaat
    15301 tgcaactacg cccgacggta cagcttattc ccatgaacag cgcgtactcg cccattacca
    15361 tcagtagcga agatacgctg gatgtctttg gtgtggtgat ccacgtcgtt aaggcgatgc
    15421 gctgatgttt gccctctgtg atgtaaacgc gttttatgcc agctgtgaga cggtgtttcg
    15481 ccctgattta tggggtaaac cggtggttgt gctatcgaat aatgacggtt gcgttatcgc
    15541 ccgaaacgct gaggcaaagg cgctgggcgt taaaatgggc gatccctggt tcaaacaaaa
    15601 agatctgttt cgtcgctgtg gtgtggtttg ctttagcagc aattatgagc tttacgcaga
    15661 catgagcaat cgggtgatgt cgacgctgga agaactatcg ccccgcgtcg agatttacag
    15721 tattgatgag gcattctgcg atctgacagg tgtgcgtaat tgtcgcgatc tgactgattt
    15781 tggcagagaa attcgcgcaa cggtgctaca acgtacccat cttactgttg gtgtggggat
    15841 cgcccagacc aaaacgctgg ctaagcttgc caatcatgcg gcaaaaaaat ggcagcggca
    15901 gacgggtggg gtggtggatt tatcaaatct ggaacgccag cgtaaattaa tgtctgctct
    15961 ccccgtggat gacgtctggg ggattggacg gcggatcagc aaaaaactgg acgcgatggg
    16021 gatcaaaacc gttcttgatt tggcggatac agatatccgg tttatccgta aacattttaa
    16081 tgtcgtgctc gaaagaacgg tgcgtgaact gcgcggcgaa ccctgtttgc aactggaaga
    16141 gtttgcaccg acgaagcagg aaattatctg ttcccgctcg tttggtgaac gcatcacgga
    16201 ttatccatcg atgcggcagg ccatttgtag ttacgctgcc cgggcggcgg aaaaacttcg
    16261 cagcgagcat caatattgtc ggtttatctc cacgtttatt aagacgtcac catttgcgct
    16321 caatgaacct tattacggca atagcgcgtc ggtaaaactg ctgacgccca ctcaggacag
    16381 cagggatatc attaacgccg ctacgcgatc tctggatgcc atctggcaaa cgggccatcg
    16441 ctaccaaaaa gcgggcgtga tgctggggga tttctttagt cagggagtcg cgcagctcaa
    16501 tttattcgat gacaacgcac cgcgccccgg gagtgagcaa ttgatgacgg taatggatac
    16561 actgaatgct aaagagggca gaggaacact ctattttgcc gggcagggga tccagcaaca
    16621 atggcagatg aagcgtgcca tgctttcacc acgttataca acgcgaagtt ctgatttact
    16681 gcgggtcaaa taaatatagc ggcaggaaaa aagcgctccc gcaggagcgc cgaatggatt
    16741 agcgaccgaa caggtcacgt tttttcgctt taaacggctg ggaaatcacc accagcaccg
    16801 cgacaatcag gtaagcgata aaaataccga gcagccactg cggcatttcc atacctaaaa
    16861 aatcccactg acgctcggcg caatcgccag aggcgacaaa cacttgcggc acccatttat
    16921 ccagcggcag ccattccggg aaacgaacca taaaatcaca ggtggcaaac ggcgaaggat
    16981 agagctgaag catggtgtgc tcgtaagtta actgcacacc gcggaacgca ctatacaacc
    17041 agataaccat cgctacataa cgcagcggag ttttcggggc gatcgcgcca atcagcgcag
    17101 cacccagaac gccgaataac gcgcagcgtt cataaataca gagcacgcaa ggtttcagta
    17161 acatcacatg ctggaaccac agcgccgtca gttccagtgc cagagcagta aacgccatca
    17221 acagccacgc gccccggcct tgtgaacatt ggttcaaaaa tcgcaacata atcatttccc
    17281 tgcaatatgc atagagtgcg cagtttaaac caattcattc gctgcgccac caggaggcag
    17341 caaaaatagg acgcaattgg acaattatcg ggcgaaaagg caaaccgggc atttatatgc
    17401 ccggtaagtt gttatcaaag cgttgctatc cagcccattt gcataaacca ttcggttaca
    17461 ggggcaagcg taaactcgac gcagagcaag ccgacgagtg tcaggacgag ggtgtaaggc
    17521 agggccatcc acaccatgcg gccataagag aggcgaatca atggcgcgag tgcagaggtc
    17581 agcaggaaca ggaacgcagc ctgaccgttc ggcgtagcga cggagggcag attggtaccg
    17641 gtattaatgg cgaccgccag cagctcgtat tgcttcaacg tgatagcgcc actttccatt
    17701 gccgctttcg cttcgttgat ataaatcgtc cccacgaaga cgttatccga aatggatgac
    17761 agcagaccgt tgaaaatata gaacagcgac agctgagcat gttccgatgc ctgcaacaca
    17821 aactgaataa ttggcgaaaa cagttgttgg tcgataatca ccgcgactac cgagaaaaac
    17881 accgtcaaca gtgcggtgaa tggcagagat tcggtgaagg ctttaccgat agcatgttca
    17941 tcggtgacac cggtcaatga tgtcgccaga ataatgactg acaaaccaat caagccaact
    18001 tccgccagat gcaacgccag cgcagtcacc agccagacgc caataatcgc ctggacaatc
    18061 agacggattt tatcctgacg ggtacgctgg tggcggcttt gatcgtcaaa ctgttgcaac
    18121 acttcgcgga ctttctccgg cagcgtttca ccgtagccaa accaacgcag cttctctacc
    18181 agcaggcagg ttaacaggcc acaaatcaga accggaacgg tcaccggcga catgcgcagg
    18241 aagaaatcgc caaaatgcca gccagccgct ttagcaatga tcaggttctg tggttcgccc
    18301 accatggtca tcacgccgcc taatgcggtg ccgacaccgg catgcatcat caggctacgc
    18361 agaaagccac ggaactgttc cagaaccact ttgtaatgct tgtcgatatg actatcgtct
    18421 tgcaggtcgg tatcttcagt acgggaagag gctacgcggt gataaatacc ataaaaaccg
    18481 actgcaacgc tgatcaccac cgccacgacg gttaaggcat cgaggaacgc ggagaggaac
    18541 gccgccgcca cgcaaaaaga gagcgacagc agcattttgg agcgaatgct taacagcaaa
    18601 cgggtaaata tgaacagcaa tagctgtttc ataaaataga tacccgccac cataaacatc
    18661 agtaacagca agacttcaag atttgccgcc acctcttcac ggacgtgttc cgcgctggtc
    18721 atgccgatga ataccgcttc gatagccaac agaccaccgg ggagcagcgg gtagcatttc
    18781 agggccatcg ccagagtgaa aataaattcc gctaccagca accagcccgc gacgaaaggg
    18841 ctgatgagga aaattaacgg gtttacgatt aagaaaatga tgagggcgag tttgtaccag
    18901 tcgggggact ggcccaaaaa attgcgccat agcgcgcggc cccaggagat ctccatgatg
    18961 gtttccctta ccttacaaat aatcaatgat gtttttatgt ttaaacgcaa agcttaacgg
    19021 tcaggcagga gtgaggcaag tcttgatagt caaggggaaa gagatgcgga aaatgaagcc
    19081 ttgatccctt ttttcttctt tttgtctgct atcagcgtag ttagccctct ggtatgatga
    19141 gtccaacttt gttttgctgt gttatggaaa tctcactatg gtcattaagg cgcaaagccc
    19201 ggcgggtttc gcggaagagt acattattga aagtatctgg aataaccgct tccctcccgg
    19261 gactattttg cccgcagaac gtgaactttc agaattaatt ggcgtaacgc gtactacgtt
    19321 acgtgaagtg ttacagcgtc tggcacgaga tggctggttg accattcaac atggcaagcc
    19381 gacgaaggtg aataatttct gggaaacctc tggtttaaat atccttgaaa cactggcgcg
    19441 actggatcac gaaagtgtgc cgcagcttat tgataatttg ctgtcggtgc gtaccaatat
    19501 ttccactatt tttattcgca ccgcgtttcg tcagcatccc gataaagcgc aggaagtgct
    19561 ggctaccgct aatgaagtgg ccgatcacgc cgatgccttt gccgagctgg attacaacat
    19621 attccgcggc ctggcgtttg cttccggcaa cccgatttac ggtctgattc ttaacgggat
    19681 gaaagggctg tatacgcgta ttggtcgtca ctatttcgcc aatccggaag cgcgcagtct
    19741 ggcgctgggc ttctaccaca aactgtcggc gttgtgcagt gaaggcgcgc acgatcaggt
    19801 gtacgaaaca gtgcgtcgct atgggcatga gagtggcgag atttggcacc ggatgcagaa
    19861 aaatctgccg ggtgatttag ccattcaggg gcgataatcc cttccgttta aaaagcaaac
    19921 ccctcaaacg aggggttttt tgttgttttt acagatttcc cattcttggc gggcaacgtt
    19981 ccagcaactc gacgctgcca tcttcgtttt gctgttcgag catcacatca aatccccaca
    20041 ggcgatgcac atgcttcagc acttctttgc gcccccgatc cagcggtgcg cgattatgtg
    20101 gaatgtaacg cagcgtcagc gaacggtcgc cgcgcaaatc cacgttccag atctgaatat
    20161 tcggctccag attacttaag ttatattgcg acgataaccg gttacggatc tcccgataac
    20221 cttcttcatt atgaatagcg gaaatctcca gataattatg ccgatcgtca tccagcacgg
    20281 tgaagaagcg gaaatcacgc atcactttcg gtgacaggaa ctggctgata aaactctcat
    20341 ctttgaaatc acgcatcgcg aaatgcaacg tttccagcca gtcggaaccg gcgatatccg
    20401 ggaaccagta tttgtcttct tccgttggcg actgacaaat ccgtttaata tcctggaaca
    20461 tggcgaaccc gagggcatac gggttgatgc cgctgtacca tgggctgtta tagggcggct
    20521 ggaagaccac attggtgtgg ctgtgcaaaa actccagcat aaaacgttcc gttactttcc
    20581 cttcatcata cagatggtta aggatggtgt agtgccagaa ggtcgcccag ccttcgttca
    20641 tcacctgagt ctgtttttgc ggataaaaat actggctcac cttacgcaca atacgcagga
    20701 tttcacgctg ccatgattcc agcagtggcg catttttctc cataaaatag agcaggtttt
    20761 cttgtggttc ggacggatag cggcgcgctt cagcaaccgt tttctcttcc tcgcgcttcg
    20821 gcagggtacg ccagagcata ttgacctgac tttgcagata ctcttcgcga cttttctgcc
    20881 gggctttctc ttcttgcagc gagatttttt gcgggcgttt gtaccggtcc acgccgtagt
    20941 tcatcagcgc gtggcacgag tccagaagcc gttctacttc atcaacgcca taacgttctt
    21001 cgcactcggt aatatattta cgggcaaaaa tcagataatc aacaatcgaa ctggcgtcgg
    21061 tccagctacg gaataagtaa ttgtttttga agaaagagtt atgtccatag caggcatgag
    21121 ccatcaccag cgcttgcatg gtaatggtgt tctcttccat caggtaagcg atacacgggt
    21181 tagagttaat gacgatttca taggccagtc cttgctgacc gtgcttatac agccgttcag
    21241 tctcgataaa ctttttaccg aatgaccagt gcggatagtt aattggcatg ccgacgctgg
    21301 agtaggcatc catcatctgt tctgaggtta tcacttcaat ctggtgcggg taggtatcca
    21361 gccggtagag tttcgccacc cggtctatct ctgccagata aacatccagc aggtcgaacg
    21421 tccagtcggg tccatcgctc aaacgtgtgg tgtccttatt catagaatcg atcgtcgcca
    21481 tacgcgcacc tcattgttgt cggcgctctc tgtgtggagc acctcatttc aagcatagaa
    21541 cacctgttaa aaaccgcgtc gccgaagaat ttttttcttt gcgatttctt attatcagag
    21601 tgccactaat ccgcttctga acggaatttt atgctggata aaaagggcgt tcagcaggag
    21661 atactaaaga cgccatattg ccgcagagtc agggagatgt gagccagctc accataaaaa
    21721 agctgtatgt tgaataatat tttcaactga gttatcaaga tgtaattaga ttattattct
    21781 tttactgtat ctaccgttat cggagtggct atgcgagttg tcatactggg aagtggtgtg
    21841 gtaggcgttg ccagcgcctg gtacttaaat caggcaggac atgaggtcac cgtcattgat
    21901 cgggagccgg gggccgctct ggaaaccagc gccgcgaatg ccgggcaaat ctcccccgga
    21961 tatgctgcac cgtgggcggc accaggtgtg cctttaaaag cgatcaaatg gatgttccag
    22021 cgccatgcgc cgctggcagt tcgtctcgac ggtacgcagt tccagttgaa atggatgtgg
    22081 caaatgttac gtaactgcga caccagccac tacatggaaa acaaagggcg gatggtgcgt
    22141 ctggcggaat acagccgtga ttgcctgaaa gcattgcgcg ccgaaaccaa tattcagtat
    22201 gaagggcgtc agggtgggac gctgcaactg ttccgtaccg aacaacagta tgaaaatgcg
    22261 acccgcgata tcgccgtgct ggaagatgcc ggcgtaccgt atcagctgct ggaatccagc
    22321 cgcctggcag aagtggagcc cgcgctggca gaagtggcgc acaaactgac gggcggcctg
    22381 cagttaccca atgatgaaac cggagactgc cagctattta cccagaatct ggcgcggatg
    22441 gcggagcagg cgggggttaa attccgcttt aatacgcccg ttgaccaact gctttgcgac
    22501 ggcgagcaaa tctacggcgt gaagtgtggc gatgaagtga ttaaggccga tgcgtatgtg
    22561 atggcgtttg gttcttactc gacggcgatg ctcaaaggca ttgttgatat tccggtgtat
    22621 ccgctgaaag gctactcgct gactattcca attgcgcagg aagatggtgc gccagtatcc
    22681 accattcttg atgaaaccta caaaatcgcc attacccgtt tcgataaccg cattcgtgtt
    22741 ggcggaatgg cggaaattgt tggttttaac accgagctgt tgcaaccgcg tcgtgaaacg
    22801 ctggaaatgg tggttcgcga tctctatcca cgcggcggtc atgtcgagca ggcgactttc
    22861 tggacgggtt tgcgcccgat gacgccagac ggtacgccgg ttgtcgggcg tacacgcttt
    22921 aaaaatctgt ggctgaatac cggtcacggc acgctcggct ggacgatggc ttgcggttcc
    22981 ggtcagttgt taagcgatct gctctctggt cgcacgccag cgatcccata tgaggatcta
    23041 agcgtagcgc gctacagccg tggatttacg ccatcacgtc cgggccattt acatggcgca
    23101 cacagctaag gaaacgagat gccccgtccg atacaggcca gcctcgatct gcaggcatta
    23161 aaacagaatc tgtccattgt ccgccaggcc gcgccgcacg cgcgcgtctg gtcggtggta
    23221 aaagcgaacg cttacgggca tggtattgag cgtatctgga gcgcgctcgg ggccaccgat
    23281 ggctttgcat tacttaacct ggaagaggca ataacgttac gtgagcgcgg ctggaagggg
    23341 ccgatcctga tgctggaagg atttttccat gctcaggatc tggagattta tgaccagcac
    23401 cgcctgacca cctgcgtaca cagcaactgg cagctcaaag cactgcaaaa tgcgcggcta
    23461 aaagcaccgt tggatattta tcttaaagtg aacagtggga tgaatcggtt gggcttccag
    23521 cccgatcgcg tgcttaccgt ctggcagcag ttgcgggcaa tggcgaatgt tggcgagatg
    23581 accctgatgt cgcattttgc cgaagcggaa catcctgatg gaatttccag cgcgatggcg
    23641 cgtattgagc aggcggcgga agggctggag tgtcggcgtt cgttgtccaa ttcggcggcg
    23701 actctgtggc acccggaagc gcattttgac tgggttcggc ctggcattat tttgtatggc
    23761 gcttcgccgt ccggtcagtg gcgtgatatc gccaataccg gattacgtcc ggtgatgacg
    23821 ctaagcagtg agattattgg tgtccagacg ctaaaagcgg gtgagcgtgt gggctacggc
    23881 ggtcgctata ctgcgcgcga tgaacagcga atcggcattg tcgccgcagg gtacgccgac
    23941 ggttatccgc gccacgcgcc taccggtacc cctgttttag tggacggcgt gcgcaccatg
    24001 acggtgggga ccgtctcgat ggatatgcta gcggtcgatt taacgccttg cccgcaggcg
    24061 ggtattggta cgccggttga gctgtggggc aaggagatca aaattgatga tgtcgccgcc
    24121 gctgccggaa cggtgggcta tgagttgatg tgcgcgctgg cgctacgcgt cccggttgtg
    24181 acggtgtaac ttgttgtaag ccggatcgga ggcaacgtct tctgggtgca aaaaaaatca
    24241 tccatccggc tggtcagcaa ctgtagttgt taatgtgaca gagccattgc ccatgatagt
    24301 gtccattaaa aggatggaca ctatttcccc ggaacctgaa ctcaccgcac aggcgttcta
    24361 cataaaacgc ttacgcttca ttgttgactc gaactcgact tcagataaat cacgctttac
    24421 ccttgatgga gcctgtacat agatttgtgt aattgcctga ttttgatatg ttcaattcaa
    24481 catcaaatga aggttaaatt atggacgaca aacaattgca ggctcaggct gcgttcagca
    24541 aagcatcgca accggcgata gatgcttcat taaatttaag attcagcttc ctcttcagcc
    24601 acccgtacgc caatcttcaa cacttcatta tcttctttct cggccaccgt ccagatcatc
    24661 ccggcaaact ctacctggtc accgacaacc ggtgccgcgc ctaacaactg ctggacaatt
    24721 tcacccagcg tttgctgctt atcacgatat tctcgcccgt cttccaggcc atatatcagc
    24781 gcaacatcag catatttggc gctggcttcg agaatgaagt caccaaagaa gcgttgatcc
    24841 agcgcgaccg gcggcgactg gctgaacagt ttaccgagcg ccggaagatc gcgttcccga
    24901 ccaattacac acaacacatc gccttcgcgc agtcgggtgc tgccggtagg atgaagcaac
    24961 tggttatcac gaaacagtgc cgcaatacgc gtctcttttg gcatatgcaa atcgcgcagt
    25021 gccgcgccca cgcaccattt atcggcactc aattgataaa caaactgctc ccacggattt
    25081 tccggatgaa tatccaggcc aacgcgtgac accggacgtc ccactggcgg aaccaccact
    25141 ttggcttttt tagccgccca cgagagtgat gttccctgca agagcagtga aaccagaacc
    25201 acaaagaagg cgacattaaa gaacagacgt gcattctcca gccccgccat catcgggaac
    25261 acggccagga tgattggcac cgcgccgcgt aatcctaccc agctgataaa cacgcgctca
    25321 cgcagattga aaccgcggaa ggggagcaat ccagcaaata ccgaaagggg acgggcgaag
    25381 aatatcatcc atgccgacaa aatgagcgcc ggaatggcaa taggcagcag atcgcttggg
    25441 ttaaccaata gccccagcac caggaacatg gcgatttgcg ccagccaggc gaggccgtcg
    25501 aaattttgca ggatgccgta gcggttgcga atcggccgat tacccagcag aaaaccgcac
    25561 agataaacag ccagaatacc gctgccttcc agcgccgtgg ttaaagcaaa aattaaaata
    25621 ccgccgctta atgccagcaa tggatataat ccggcaggca gggcgatgcg attaatcatt
    25681 tgcagcagta aataaccacc gccaagtccg atgacaatcc cgaggccaaa ttgctgcaga
    25741 atatcgacca cgaacatcca actgacactg ctttcatgtt gctggatcat cgcaatcagg
    25801 gtaatcgtca gaaagaccgc cattggatca ttactgccgg attcaatttc cagcgtcgag
    25861 ccaacacgtt cgttaagccc cttaccaccc agcagagaaa agaccgctgc agcatcggta
    25921 gagccgacga tagcgccgat taataagcct tcaatcaaat caagattaaa cagccacgcc
    25981 gccatcatgc cggttaaacc agaggtgata agcacgccca gcgtcgccag cgacagtgcc
    26041 ggtcctaacg ccacacgaaa ggaactggcc tgagtgcgca tcccgccgtc gagcaaaata
    26101 atcgccagtg ccaggttact taccatgtag gcgaaggggt aattatcaaa cgggataccg
    26161 ccgacgccat cgactcctgc cagcatgccg atcgccagaa aaataaccag aataggaatg
    26221 ccaagacggg aagaaaatga actaagtaat atactgctgg tgacaaggat ggatcctaag
    26281 atgaaaaggc taattattgt tgtggcatcc aacggtcggt tactcctgat tacgctgtct
    26341 cttatataaa ccctaccata ttagcggcag agacagcgtt ttacttagcc ctgaagcgtg
    26401 atttttttac attttaagaa caggatgacc gctaatcgtc aactgagtgc cttcccgggt
    26461 gttattcaga atggcatgtg cgcccaacgg cagcgtaacc gtgcgttgtt catgaccaaa
    26521 atcgaggccg gtaatgagtg gaatcgacag gcgggaacgc aaaaacgcgt acactgactc
    26581 caggttgtaa cctgcgtcat aatcattggg cgtgctgccg ctaaagctac cgagaataat
    26641 cgccttctga cgtggcaaaa ttcccgcatg atagagctgc aacagcatac gttcgacgcg
    26701 gaaagggtgc tcgttaatat cttccagcac cagaataccg ttctcaattt ttggcatcca
    26761 cggtgtacca atcagtgaaa tcagcatcgc aagattgcct ccccacaacg tgccttcggt
    26821 ctgacatgtc gggccttccc cttgccattc aatggtgaag gtttcattgc gtaacgccag
    26881 ccagaaatgg tgctcggtaa aggcgttcag ttcatccgcg ccaaagtttg ctaccagcat
    26941 ggggccgcta aaggtgatga cattgccttg cgccagaaga ccgcactgaa tggcggtaaa
    27001 atcgctatgc ccgcatatga gcaacgggtc atattgttgg cgagcaacca gtgcctgcca
    27061 gtcaatatct gccagtaaac gactggcacc gtaaccgccg cgtacagcca gcacgatggt
    27121 gttgggggtg gtcagtctgg caagggaatt gagatcttcc aggcgctccg tttccgtacc
    27181 cgcaaaacgc tcacaacgac gggcaataac ctcgacgtta ttgacctgat gcccctcgtc
    27241 ggtaaggcgc tggataccac gcagcgcggc gtgctgttta atgcagtaac ccgatggggc
    27301 aattaagtga aacagagaca tggcaattcc ttgctgacaa cagaaacgaa atgtatatca
    27361 tgccgcttag gtgtgccgtt gtcacctcaa cggcgattcc aggctataag gatagaagaa
    27421 gtgaaattga gatggtttgc ctttttgatt gcgttattag cgggttgttc atcaaagcat
    27481 gactatacga acccgccgtg gaacgcgaaa gttccggtgc aacgtgcgat gcagtggatg
    27541 ccaataagcc agaaagccgg tgcagcctgg ggcgtcgatc cacaattgat cacggcgatt
    27601 atcgctatcg aatcgggtgg taatccgaac gcggtgagta aatcgaatgc cattggtttg
    27661 atgcagttaa aagcttcaac ctccggacgt gatgtttatc gccgtatggg ctggagtggt
    27721 gagccgacga ccagcgagct gaaaaatcca gagcgtaata tttcaatggg ggcggcttac
    27781 ctgaatattc tggaaaccgg cccgctggca ggcattgaag atccgaaggt actgcaatat
    27841 gcgctggtgg tgtcatacgc taacggggca ggtgcgctgc tacggacttt ctcgtcagat
    27901 cggaaaaagg cgatcagcaa aatcaacgat ttggatgctg acgagttcct cgatcacgta
    27961 gcgcgaaatc atcctgcgcc gcaggctccg cgctatatct acaaacttga gcaggcactg
    28021 gacgcgatgt aaatcagtcg cgcactttgt ccgctttttc ccgggcttct cgctcgagag
    28081 agaaaataat ccgctgtaat tgccgctcca ccgtcgggct gacgttaaga aaacggaagc
    28141 tcagacgggg agtggtgatg gtttcattct tgccatcaat cactttgcgc tcgctgatgg
    28201 agattaactg ggcgtcaaag tgaaaaacac cccattgccc catgttgact tcaatctgag
    28261 cgaagcgcat gccttcttgt aattcggcag gctttgctgt ttccagtaat gcgcccatgc
    28321 cgcctaacga caaatcatac aggcggaaac gtaacgtact gttatccgcc agtttggtct
    28381 ggcaaaaata aggcggatgg agtggggcgg agatgcggaa atatcggcgt cgttgtacaa
    28441 accataaggt gggaggcggt acggtaataa atgccggaag ctgcaagtat tcactctgct
    28501 gtagttgttc aacagtaaac tcgactttcg caccctgagt ttcggcggta atggtaatgt
    28561 gctgtgcctt tagcacggcg atgttgtctt cggcttgact gccgaaatcc agcaccagtt
    28621 tatccggggt tattgccagt aatttgctga tcagctgccc gccattccaa ctgagacgca
    28681 aaggaattgc ggctttgtgc aaatcgcgta acacgcccag gacggctaac ggattttgtt
    28741 tcaggaactg ctcatggtaa tgactcacgc ggaaaaactc ctgactgaca aactgtcttt
    28801 ggtttatcgg tcacagttaa caaaacttaa tacaaatgcg tgaatatttt ttacatgttg
    28861 ttcttaaatc agccgacata cgcccagcat cgatcccgcc cttgcctata cttagagcgt
    28921 tgacgtaagc atttcttgcg tccgattcat cgaaacgagg gcctgaacat gggaattatt
    28981 gcctggatta tttttggcct gatagccggc attatcgcca agctaatcat gccggggcgt
    29041 ggtggtggtg gatttttcct gacctgtatt ctcgggatag tcggtgcggt ggtcggcggc
    29101 tggctggcga ccatgtttgg cattggcggc tccatcagtg gttttaatct gcacagcttc
    29161 ctggtggcgg tggtgggagc tattctcgtt ctgggcatat tccgcctcct gcgaagagaa
    29221 taagattttc ataaggcgga tagcgataca gatgccgcta tccgctttca catcagaacg
    29281 tatactcgac acctgcttta cgggtgaaaa aaatcaatat agcacttcga tatccgataa
    29341 agtcggttcc cggtaacaac aacagaatcc ttttttaatt attgtttcgt tgtttttggt
    29401 gtgattcaga aaataaatat tttagacagt agccagtcag gcgttaaaaa tggggcggat
    29461 agtgaggcgg ttttggctgc gcttattgaa catttagaat tgatcaatcc ttctggcaga
    29521 ttaagccaaa atacccgttc ggcaatgctt cagttacgcg aagaggaatg gtctgaattc
    29581 tttttttggt tgctaaattc tctggagtgt ctggattatg tgattattaa tttaactcca
    29641 gaaagcaaaa aaacgttgat gagtgaacac cgtaacaata ttcaggtagc aatcgatgct
    29701 ttatataggc aacgtcggtg taaatcaccg ggggatgata gtgaaacact tacccgtaga
    29761 aatgacgcta tcttcggcaa ccacgtatgg caaacatttg cgcaatactt cccgccagga
    29821 cttgaaaaac cgtctgtgta gaaaaaacca tagctatggt gttatctgcc gacaacgcca
    29881 tccggagaag ccgaatgacg ttgtcggagt aagcgctggt taaggtgtgg gttgtgcctc
    29941 tttggttgag ggttgcgtcg ttgctgactt aacggtcgga cgcgtcgccg gaacattgtc
    30001 acacggttgc tctttcgggc agatcaaatc cagcattttc agcgtcacgc cattggtcca
    30061 gccaaagcca tcctgtaatg gatattcgcc accgccgccc cccgttccgg tggtgctgac
    30121 atcatatttt tccaccagct ttttctcccg gtcataggtg tgctgaacat tggtcaggaa
    30181 gtgccagcta atgtccatcg caacctcttt ttgcccgtag ttttgtaatc cttctgtcgc
    30241 gacccactgt aacggtgccc agccatttgg cgcatcccat tgttgcccac ttttcaccga
    30301 cgtggtgttc aggccgccgg gttgcagcag atgtgttttc gtcgccgtcg ccattttgtt
    30361 ggcgcgatct ttcgctgccg cattgacgta cagcgggaac agggcggccg cggttaactg
    30421 attgcgcact ttatgacttt tcaggtcgta atcggcatac cagccttgtt gaccgttcca
    30481 caggtatttt tcgattcctt tttgacgggc attcgccagc gtttcgtact ggtttgccat
    30541 cgcgttatct cctgcagctt tgctggcgcg ggcgaggatt ttttccattt taaacatcag
    30601 gctgttcaga tcgaccggta cgatgctggt ggtgcgtaag gtatttaact gctgcgggtt
    30661 gtccatccag cgcgagctga aatcccagcc agacgcagcg gcagagcgca ggtcgcggta
    30721 aatttcagtg gcaggtcgat tcggattgct tttggcggtg gcaatatctt ccacccatga
    30781 ctctggtcgt ggcgtatcgc gatcgtccca gtagcggttg agaagggtac catcctgaag
    30841 tttgacaacg cgtttttcct gttgtccggc ttgcaggttt tcaacaccgt ccatccagta
    30901 agcatattct ttttgcattt gcggcaggta ttgcttcaac gcggcatcgc cttcatgctg
    30961 cgccagtaac tctaccatca gggcaaagaa gggcggttgc gagcggctta aatagtaact
    31021 gcggttgccg ttgggaatat gaccgtaagt gtctatttca tgagcaaaat tggccaccat
    31081 atccgcgact ttatcccagt gaccgctttc ggcaagtcct aacatggtga agtaactgtc
    31141 ccagtaatat acctcgcgaa aacgtccgcc cggcacgaca taaggttccg gcagcggtaa
    31201 cagagaatcc catttttcgg tgttttcggt agaacgcgtt aataccggcc aaagtccgtc
    31261 aatatgttcg cgcagtgact gcccctctgg cggaacatat ttctcgcctt ctttcggcag
    31321 ggtgaaattg acgttaacga aatggcgcag atcaaatccg ctctggtttt gctgcatccg
    31381 ataatcagca aggatcatca gcggatcgct gttcggcacg gcatcggcaa aggttttttg
    31441 gtccggaaaa agtttggcgt tttgcacatc attaaacagc ggccctaata aaatatcagg
    31501 cggctgtggt gttaccggtg tttcttctgc ctgcaccgat agcgcagcga aacacaaaaa
    31561 gatacaggct ggaattaacg ccattttttg cgggtgagaa ggtgcggggg atttcatcaa
    31621 tcattctcct ttggcgaaac cgaataaaac ggtattcaag tatcagaaaa ccttagttca
    31681 ggatcgcact agctgcatga tcgaaatctc attctgcgaa ctgtcaggca ttttagaaaa
    31741 gggaattaaa gggataaata acggcgaaat agagtgatca aataacgtct gtttattagc
    31801 cagccagcgg cgttgggttc gtgattagcg aagaaaaatc atcataaatt ggtgaaaaat
    31861 ataacaggta gttaaaaacc attagtgctg agtaaattgc cggatgacat cagaacgatg
    31921 ccatccgaac agtggcttaa ccctgacggt tgaaacgttg cgttttaacg tccagcgtta
    31981 gcgtttcttc tggttgtatc gcatacagtt tctcaccctg ctggcaaatc cagccaatcc
    32041 ccagttcacg ggcgattagg gcgctgtggg ataccggact tccggcgcta aggcagatac
    32101 cttttacaac cgccggatcc agttgcagta ctgtggaagg ataaatgttc tccgccagta
    32161 gaatagttgg cgagttaaac tgcgggagtt cttctttcgt ttgggtcagg tggaccaggg
    32221 tgcgatgcag aagatcgtcc acatcaatat agcgagcttg tagatattca tcatccagtt
    32281 gctggtattg ctggctaagt tctttaagaa cttgctgcca ggcatattct gccgtgcaat
    32341 gttcatgctg aaggagttcg cttgccgccg ccagcagttc cggatcatct aacagtgtat
    32401 ggtgaccaga aaagattgcg gcaatatcgt caagcccgct ggcttctgct ttcgctgtta
    32461 acgtcatcag atctaataac gtgaagtcaa tagcctggcg taatcgatct tgttcttctt
    32521 ccacggtcag ggttgatttt gcctgtaccg tacataaaac tggttgataa taaaaggctt
    32581 tacccgaaac aggcggaacg ggacgcagag taggtggagc gacttcctcc gtttcaccaa
    32641 agttatcttc agccagctga cggaaagcga tcagtgcctc ttcagcttct ggccctttcg
    32701 caatcaggcg cagcgtatcg ttatagcgaa cttgtagtaa cgcaatctgg ttaatactct
    32761 ctggtgtgac gcatttgccg tttttttcca gcaacatatc ggcattaaat gtcgataagg
    32821 tataaaccag ccgggaggcc ggacgtacat gcaggccgtt acggtttttt atgaccaccg
    32881 ccagagaacg ggcttcttca tcgtacgcag gacatgtgtc agagatttca gtgtcggagg
    32941 acggtaaacc cagttgttca cgtttggctt ccagcgcatg catagcgtca aagataactt
    33001 tgtcgatatc cgctcccgag gccgcgctga ccgttgctgc cagtgtacct tcgaccaacg
    33061 gcgcagcaca caaacgtact tttgcggcga tcttgggagc cagcaattcc agcgcagttt
    33121 cagcactcaa taatgcgcta cccatatcca tcatgaccag cacatggtcg gcatcagcaa
    33181 cagattcgat ggcctccatc actttgacgg catcggtacc aataggattt tgtggatcgt
    33241 caattcccgc ggcaatggcg attttacaac tatcactcat taacatctga cgggctaatt
    33301 caccgacacc ttctcccagt cggctgctat gtgaaactat gaccaggttt accatcacca
    33361 attccttact cttttgcggc taacgccaac atttgcatca taaacatcac cgaggtcgcg
    33421 ccgggatcct ggtgaccaat actgcgttca ccgagataac tggcgcggcc tttgcgggct
    33481 tgcatcgtaa tcgtactttg tgcagcggat tcggcgatgc tactggcagc ttcgagcgcc
    33541 actggaacag agagattttg ctcgctggac tgacgtaacg attccaccac cggcacccac
    33601 acatcacaca tggttttatc gccaggttcg gctttcccgc gactgattac gccgtccgcg
    33661 ccatcgcgga acatctgata aagctcttcc agtgtcaggc tttgccgtgc ctgggtcgcc
    33721 tgtgcggcgc ggataaagaa ggtaccgaac agcggaccac tggcaccacc gacgctggaa
    33781 agcagcgtca taccggtatt cttgagaatg aaaccgatat ctttatctgc gatagcaggg
    33841 agtttttcca ccactttgct aaagcctcgg ttcatattta gcccgtggtc agcgtcgcca
    33901 atttcgcgat ccagtccggt aagatactcg ctctcggtgc tgaaaatatc gccacaacga
    33961 gtgagccagt taacaatttg agttctgctc agtgacattg ctttctcctt atttacccca
    34021 gttaagggcc ggggtgtgga ccggggcgtc ccagagtgcc agcgtttcgt catcaacttt
    34081 cagtaaggtg attgagaaac cggtcatatc cagtgaggtg cagtacgcgc caattaaatt
    34141 acgttcgata gtcaatcccg cttgctggca acgtgtggtc aggcggttat agacgccgta
    34201 cagctcagaa agcggagttg cgccaagatt gttaaccagc gcaatcaccc gatcgccaga
    34261 ctggagcggt tgtttggttt gttgttcttc ctgccaactg ccttgttgat aatcccagaa
    34321 acgcaaagtg cgatggtatg agccatttac cagcagggtg tcgaacattt catcgacggt
    34381 ttgatcaagg gaagagaagg ggcggcggtc aatacccggc tcaccatgaa tgccgacgcc
    34441 aaactccatc tcattatccg ccagggtaaa agaaggtttg cccgcggcag gaacggtaca
    34501 ggcaccgaga gcgataccta ttgagtggcc ttgattattc agcttacgcc ccagttccgc
    34561 acaggcgtcc agtgagtcgc cacgctccgc cgctgcgcct acgagttttt caattaatac
    34621 ggtgttggca acgccgcgtc gcccggcagt ataaagactg tcttttaccg caacgtcgtc
    34681 atcaatgacc acagtggtca cttttacgcc gctatcgtgc agtaactcgg tcgctgtttc
    34741 aaagttaaga atatcgccgg tgtaattttt gataatcaac agtacacctt cgccgccatc
    34801 aacttgcatg gcgcattcaa agattttatc gggcgtcggt gaggtgaaaa tttcgcccgg
    34861 acaggccccc gaaagcatcc cctgaccgat atacccacag tgcatcggct cgtgtccgct
    34921 gccgccaccc gacagcaggg cgacttttcc tgcaacaggg gcatcagctc gggtgacata
    34981 caccggatcc tgatgcagtg tcagcgatgg atgcgctttc gccagtcctg ccagttgttc
    35041 gtccagtacg tctcgcacat cattgatcaa ttttttcatt attttgctcc agcaattacg
    35101 gtagggcatg gatgatgttc aacgacacgg cgaccactga ctgccgatga atccattgtg
    35161 catcaggcaa gggaaaagaa aattagccca aattatgttt catagtgaaa catatgcttt
    35221 aatgaatgtt ccatattgaa acttttacgt gtattaatac ttaaaattgc gagccggaac
    35281 accttttgtc ataagggatg cgggatatga gtggcgcttt taacaacgat ggtcggggca
    35341 tatcaccctt aattgcaacc tcctgggagc gatgcaataa gctgatgaaa cgggagacat
    35401 ggaacgtacc acatcaggcc cagggcgtga catttgcttc tatttatcgg cgtaagaaag
    35461 cgatgctgac gctcggtcag gctgcgctgg aagatgcctg ggaatatatg gcaccgcgag
    35521 agtgtgcgct gcttatcctc gatgaaaccg cctgcattct cagccgtaat ggcgatccgc
    35581 aaaccttgca gcagctaagt gcactgggat tcaatgacgg cacgtattgc gccgagggaa
    35641 ttattggtac ttgtgcgcta tcgttagcgg ctatctctgg tcaggccgtg aaaacgatgg
    35701 ccgatcaaca tttcaaacag gcactctgga actgggcctt ttgtgcaacg ccgttgtttg
    35761 acagcaaggg ccgattgacg ggaacaatag cgctggcgtg tccggttgag caaactaccg
    35821 cagctgattt gccgttgacg ttggcaatcg cccgcgaggt cggtaattta ctgctgacgg
    35881 acagtttgct cgctgaaact aaccgtcatt taaatcaact taatgccctg ttagaaagta
    35941 tggatgatgg cgtgattagc tgggacgagc agggtaattt gcaatttatt aatgcccagg
    36001 cggcgcgggt cttgcgcctt gacgcgacgg caagtcaggg acgggcaatc actgaactct
    36061 taacgttacc cgccgtattg caacaagcaa taaaacaggc acatccgctc aaacacgtag
    36121 aagcaacctt tgaaagccag caccagttta ttgatgcggt gataaccctt aaaccgataa
    36181 tagaaacgca gggaaccagc tttattttgt tgctccatcc tgtggaacag atgcggcagt
    36241 tgatgaccag tcaattagga aaagtcagcc ataccttcgc tcatatgcca caggacgatc
    36301 cgcaaacccg ccgcttgatt cattttggtc gccaggcggc gcgcagtagc tttcctgtcc
    36361 tgctttgtgg agaagagggc gtgggcaagg cactgctaag tcaggcaatt cataatgaaa
    36421 gcgagcgtgc tgcaggtcct tatatcgccg tcaattgtga gttatatggt gatgctgcgc
    36481 tggcggaaga atttattggt ggcgatcgca cggacaatga aaatggccgt ctgagtcggc
    36541 tggaactggc acacggcggc acgctgtttc ttgaaaagat tgaatatctg gcggtggagt
    36601 tacagtctgc tttgcttcag gttatcaagc agggggttat cacgcgactg gatgcgcggc
    36661 gtttaatacc aattgatgtc aaagtgattg caacaacgac cgcggacctc gcaatgctgg
    36721 tggaacaaaa tcgttttagt cgccagctgt attacgcgct gcatgcattt gaaattacca
    36781 tcccgcctct gcgtatgcgg cgtggcagca ttccggcgct ggtgaataac aaattacgca
    36841 gtcttgaaaa acgcttctct acgcggctga aaattgatga cgatgccctc gctcgcctgg
    36901 tttcttgtgc atggccaggc aacgattttg aactttacag cgtcatcgag aatcttgctc
    36961 tgagtagtga taacgggcgc attcgcgtca gtgatttacc tgaacatctg tttaccgagc
    37021 aggcgacaga tgatgtcagc gccacccgcc tttccaccag tctgtcattt gcggaagttg
    37081 aaaaagaggc aattattaac gcagcccagg tcacaggcgg tcgcattcag gaaatgtcgg
    37141 ctttacttgg gatcggccgc actacgctgt ggcggaaaat gaagcaacat ggcattgatg
    37201 cagggcagtt taagcgccgg gtatgaaaga cagaaacgat ttctgataca tcagagtgat
    37261 ctgtatttca ttccggcgca cgctaacaat tttcagcatc gtttaagggc ttgtctatcc
    37321 cgcacttaaa agctgaagcg atatcctccg ttgacttgct tctgatcaaa gaggttaccc
    37381 tgcgtgtaat ccgcttcgag atagaatgtg tgctgtttgt tatactgtgc actgactccc
    37441 acgccgttat tccagccatt acctttgaaa ctgtacttct cccggaagtt gttcaacaga
    37501 tattcggtat cccctgaaaa ctcgcggata gcgccagtct tcacatagac attcagctgg
    37561 ctgttgcctg cggtgatgtc ataccccagt atcatgctgg cacgccccag cagcgattcg
    37621 tagtgattca gatgtatatt gaggccatta ctcgccttca tagccatctc attctggtgg
    37681 ctgtatgtaa gctgggtttg cggctctata tagaacccat aaccagtagg ggacaggttg
    37741 aacctctgcc cggcttccag ggagatgctc attccattcg cagtgccgtt ggcgttaacg
    37801 ccgttgttct gactgtccag tacgtggaaa ctatttttct ggcgcgatgc ttttataacg
    37861 agatcgctgt aaaaaccgtt ttgtgccatg taactggcgt acattcccat gtagtctgaa
    37921 cgtgcggtac cgtcgcctcc gctatagtcc ggcgatgcat gtgttgagcc aatatacaga
    37981 ccgacataca acggcattac atcagagaga cgtttatccc caccaaactg gataccgctg
    38041 taacccatgt caaagccgct cagtttgcca ctggcaaagg agtccaggct tcccccataa
    38101 ctgcgcaacc agatattacc gtctttactc tgatttcgca ggtcacccat ccgttgcatc
    38161 agcgtacggt tttcaacata gttcaataag taaccgacat tgagataatt gccgccagca
    38221 tctgcggtcg tggtgggttt aggcgtggga gcgggttcag gcgtaggatc ggggttgact
    38281 atgggaggct gagcgggagc tggtgtgggt tcaggattag gagtcggttc cggaactgtc
    38341 ccggaagcgt aaagctccca gttagtgaca tttttacgca catcgtacag atatcccccc
    38401 aactcaacct gagaagacgc gctgaacgag gccgcgccgt cagtggtttt taccactgtc
    38461 agaacttcat ttcccgttgt ggcctcgctg ccctggttgc ggatagccaa tacgtgatta
    38521 ccagcactac tcccgctgat attcaataaa tcccctttat tattaacgcc attaccctcg
    38581 ccaacaacat cagcacgcat aataaaggta ctgttaccgc tcaggttctc tacgtttaat
    38641 gtggcaaatg tgccggcagt tgacccgtgg ctggcaaaat cgacagttga atggctcagc
    38701 gccagcgtgt cgagattaga attacttgtt acgttccaga cgctgttatt cattgcaacg
    38761 tccagttttc cgccattgac attatcgctg agggaggaac ctgtccaaac cgacccaggg
    38821 tgcatatcca gattgattag cccacctttg gaaagaacgc taccgttgat aagcatacga
    38881 cccgaggcgt tgatgcggct ggcggcataa ccatcgtcat gttgcgttgc gatggccatc
    38941 tggtcgggtg tactcatatc aatgaccaga tcgctcgtga ggtcgatctg gctgttggtc
    39001 atggcataaa tccctctggc tcctgccgcg ccggtgatag ccaaactgtc tccggttatt
    39061 ctaccgccgc tgagcgccca caaacccagc gccagactgc cattacgatc aactgtaata
    39121 tcggtatttt gcatgttgac aacagccgtt gccgtctggg ccgaggcacc ataagaaccg
    39181 ccggaaaaga tgctgtttcg ttgcgctgcc gtgccagtaa aattgattat cgcgtctgaa
    39241 ccactggtga cgaggccacc gccctgcgcg gaagaaatat ggctatctgc accgatagtg
    39301 gttgtaccac cacgaacttc gacgccattg gccgcggctc cagttacatc aacagtgagt
    39361 gcattcgcgc taacctggcc aaagctccag aggccgtgag cattatcgcc attggtttta
    39421 atggtactgt ttgttccgag atcgacaaca gagtttttct gtacgtttat ccccatggcg
    39481 ctgtagccct gaacatcgat tgtcaggtct gtagccgtaa aacgcgcagc accattggcg
    39541 ttattgccgt tgagaccacc gatataaaca cctgtacttc catcggtcgt gattttactt
    39601 ccgcttccaa gatcgacact ggtgccatag tcattgatgg ttaggcctat accgttcgag
    39661 ttttcaatgg tgaattgagt ggctgttagt gttgagctat gcccaataat gatgccgtca
    39721 tcgttactct taatggtgct gcctgtgcct aagtcagcat gggtatagtc accaattaag
    39781 ttaatgccga tggcagaggt ttgaccaaca acatctactg tgagtcggtt agctgtcagg
    39841 gagctgtttt tgccctgaat aatgattcct tttgcatatc cagaggcgtc gttgacattg
    39901 acggtgacat cattacccag gttgatattg ccaggcgtgt ttcctgctgg ggtcattaca
    39961 ccatagaggt ttcctgatgg atcggcggta tctcctgtaa ttctgtcacc atcattaata
    40021 gtaatattgg tgccatattg acttcctgta tcagcagcta tcagcagggg ggaaaagata
    40081 attgcattca aaccaatagc gccaaatttt atcagttgaa ttgagggcga acggattttt
    40141 aattcagcga gacgatctgc tttggtattc ccattgtgtt gtttgatgcc cataccactc
    40201 ctatatagta cccagactat gggtctatgg attttgtcat tttgtttaaa tcaatgaata
    40261 atatcctctt atcatcatga tatttgtatg gtgcaatcat gatgagcttc tcagagaata
    40321 attctctatt accgggcaaa atcaggaaca caggaattaa tatatattta acatccccct
    40381 tacaaaggtg agtttatatc gagttattcc ctaagttgtc gttacttatt ccaattcgtt
    40441 actataaata tggcaaaaat attacaacaa acagcggtta aaaaattttg tgaagatttt
    40501 ttgtaacaat cctaattagt tatgggaatt tcgcttgatg tatctgcata acaaaaacaa
    40561 tatgcgccat ttttgcatta ctaataagaa aagcaaaaaa catcgatatt catttttggt
    40621 caatgggttt tctggcctat cgttgttatt gtgctactgg ttatcatttt catcttgcca
    40681 gcatattgga gcgtgatcaa ttttgatcag ctgtgaaggt ggctgatgtg tagtgataca
    40741 aaaacttacg ttatatcact gctaatgaga tatccgtcat ctgaacactt atgtctttaa
    40801 ttattctcgt ggttcactat aggcaataag cacaaaagtg taggatgtta caagaatgat
    40861 taggactcgg tgaaatgaaa aatccacgca attgcgtgga ttttatatac ttttgcgctc
    40921 ttcatgagat ttagcgaaac ctcatgagac aataaattaa ttagacgttg aaaaggaagt
    40981 tcatcacatc gccatctttc acgatgtaat ctttaccttc tgcacgcatt ttgcctgctt
    41041 ctttcgcgcc ttgttcacct ttgtaagtga tgaaatcttc aaacgagatg gtttgtgcac
    41101 ggataaagcc tttttcaaaa tcagtatgga ttttgcccgc tgcctgcggc gcggttgctc
    41161 caaccggaat ggtccatgca cgcacttctt tcaccccagc ggtgaagtaa gtttgcaggt
    41221 tcagcagctt ataaccggca cggatcacac ggttcaggcc cggctcttcc agcccaagct
    41281 cctgcataaa ctcgtcacgt tcttcgtcgt ccagttcggc aatgtctgct tcaacagcag
    41341 cacaaaccgg aaccacaaca gaaccttctt tcgccgcgat ttcacgcacc tggtcaagat
    41401 atgggttgtt ttcaaaaccg tcttcgttga cgttggcgat gtacattgtt ggtttcagcg
    41461 tcaggaagct caggtaacga atagccgctt tctcttcagc gcttaaatcc agcgcgcgca
    41521 gcatacctgc attttccaac tggggcaggc atttttccag gaccgccagc tcagctttcg
    41581 cgtctttatc gccacctttg gctttcttct gtacgcgatg aatcgcacgt tcgcaggtgt
    41641 cgaggtctgc cagcgccagt tcggtgttga taacttcaat atcgtcagcc gggttaactt
    41701 tgccggaaac gtgaatgatg ttgtcatttt caaagcagcg aacaacgtga ccgatcgctt
    41761 cggtttcacg gatgttggtc aggaactggt tacccagacc ttcgcctttc gatgcgcctt
    41821 ttaccagacc ggcgatatcg acaaattcca tggtcgtggg aagcgtacgc tgcggtttta
    41881 cgatttcagc cagttgatcc aggcgaggat caggcattgg tacgacgcct gtgttcggct
    41941 caatggtgca gaatggaaag ttggccgctt caataccggc tttggtcagc gcgttgaaca
    42001 gggtagattt cccgacgttg ggcaaaccga cgataccgca tttgaatccc atgatttaaa
    42061 tcaccttaat atcttaataa tcaacctgtt attgctaaca gattgcagaa atggaaataa
    42121 ctttgcctat tatacacggc actcggcaaa aatgccgcag acaacgactt attgcgcttt
    42181 aaaggcgtgc aatcggttcg ttgctttggt caagccatct gtaaaccaca tttcagtaca
    42241 acgcgccgct tcgtcaatgg cttcatcaat taacttctgt tcactaacag gcggtttgcc
    42301 taacacaaaa ccgacaactt tatttttatc gcccggatga ccgattccga tgcgtaaacg
    42361 gtgaaagtta gggttattac ccaatttact gatgatgtct ttcagtccat tgtgaccacc
    42421 atggccaccg cccaatttaa atttggcgac gccaggaggc agatccagtt cgtcgtgggc
    42481 caccagaatt tcgtccggat taatgcggaa aaaactggcc atcgccgcaa cggctttgcc
    42541 gctgagattc ataaatgtag tcgggactaa caggcggaca tcttcgcctc caagagtgac
    42601 tcgcgaagta taaccaaaga atttagcctc ttcgcgcagc ggagcgcgca aacgctctgc
    42661 cagtaagtca acgaaccagg caccagcatt atgtcgcgtt gcggcgtatt cagcaccggg
    42721 gttcgccagg ccgacaatca atttaatcgt cacgtttttt tgtcctgagt gtgtacataa
    42781 ctggcgcgta gtttactggt tgcggccccg cttgacaaaa aactgcgtat caaatgcaga
    42841 taacgtaata attgcctgag tggactatta gaaagtcaag gtgttcaggc gtttatttgt
    42901 aaagttttgt tgaaataagg gttgtaattg tgatcacgcc cgcacataac ccacagggtg
    42961 ttgtctatac tttacacata aggaagaggg gtattccctg ttacaaccca gaaagttccg
    43021 gaggtgacat atgaaacgca aaaacgcttc gttactcggt aacgtgctca tggggttggg
    43081 tctggtggta atggtggtcg gcgtggggta ttcaatcctc aaccagttac cacagtttaa
    43141 tatgccccag tatttcgcac atggtgcagt gctaagtatt ttcgtcggtg ccattctctg
    43201 gctggcgggt gcccgtgttg gcgggcatga acaggtgtgc gaccgttact ggtgggttcg
    43261 tcactatgac aaacgttgcc gccgtagcga taatcgccgt catagctaac aaataatgca
    43321 gtttgctgac cagtcagttt gcgctgactg gtcaatctcg tacttataaa tccgccatcg
    43381 ccgcgcgacg attcgggaag aacgcgaggc gtcccgggat cggttgaatg cctgcgcgag
    43441 ccatagtgcg cagtggctgg aattccacgt tgcacacgcg cagttcacat ccttcgggca
    43501 gacgcttcac aaaacgctgg aacgcatcaa gaccaccagc atcaagtacc ggaacggcat
    43561 cccacttcag aatcacaatc cgtttgcctt caagacgtga ctccaggtcc gtgaataagc
    43621 cttcagcagc agcaaaaaac agcgggccaa taacgcgcag aaccaggaca tcgtctggaa
    43681 catctacgac taccggtgcc aggcgagtca tacgtgcgat acgacgcata aacagcagcg
    43741 atgccagcac gatccccacg ctgatggcaa taaccatatc aaacagcacg gtcagcgaca
    43801 tgcacagcag catgacgatg atgtcatctt tcggcgcatg acgcagcaag tcgaccactt
    43861 tatgcgcttc actcatgttc cacgccacca tcaacagcag ggctgccata gcggaaagcg
    43921 gcagccagga gagcagcggt gccagtacca gcagggcaag aataaccaga atagagtgga
    43981 tcaccgccga gataggggac gttgccccgg cacggacgtt agcggcagaa cgcgcgatgg
    44041 cagctgtagc ggtaatacca ccaaagaacg gagcgatgat attccccagc ccctgaccaa
    44101 ccagttcgct attcgccttg tgtttcgtcc cggtcatacc atccagcacc acggcgcaga
    44161 gcagagattc gattgcgccg agcattgcca ttgagaatgc cgcaggcagc agtgtgcgaa
    44221 tagaatccca ggttagcgtg aattctgaat taggcagatc ccacggcagc accagttgcg
    44281 gcagcagttg cggaataccg ttaccctgag aaccatcggc caggacgtag tggaattgcg
    44341 aaccgatggt agcaacatgt ccgccgagca ggttaacaat ccccatcacc gcgcaaccag
    44401 ccagcaaggc cggaagatga ccgggtaaac gaatgcccag acgcggccag aaaaccagaa
    44461 taccaagcgt cacaatgcca atggcagcat cacccacatt aatggtcggc agcgccataa
    44521 ataatgcgcc gactttttgt agataatgtt ccgggacatg ggccatttgc agaccgagaa
    44581 aatctttaat ctgcatggta ccgatggtga tcccgatacc cgaggtgaaa cctaaggtga
    44641 cggaaaccgg aatatactca atcaggcgac caaagcgtgc cagacccatc agaatcaaaa
    44701 agatccccga caacaaggtc gcaaccagca gtcctgccag tccaaactgt tgcgaaacgg
    44761 gatagagaat taccacaaat gccgcagtcg gaccggaaac gctaaagcgt gatccacccg
    44821 tcagagcaat gacaatcccc gcaacagctg cggtatataa accgtactgg ggtgccacac
    44881 cactaccaat agccaacgcc atcgccagcg ggatagcaat aatcccgacg gttatcccgg
    44941 caatcaggtc acgggtaaac cgtgcggcag tatatttttc tttccagcaa gcgtcgatca
    45001 gagcgcggaa aggcatcaca tgtgaggaaa atattttgtt cacaataatg tttcatccgt
    45061 gagcgcatca tctgtcaact aaatggcagg tgaaggaggc ataggtcata caaatggata
    45121 ttacagacaa aaaaacccgc cgcagcgggt ctttgagccg ggttcgatta gtgttcgaac
    45181 atggcagaga tcgattcttc gttgctgata cgacgaatcg cttcggccag catacctgac
    45241 agggtcagag tacgcacgtt cggcagtgat ttgatttcat cgctcagcgg aatggtatcg
    45301 cagacaacga cttcatcaat tacagagtta cgcaggttgt tcgccgcgtt gccagagaag
    45361 atcgggtgag tcgcgtacgc aaatacacgt ttagcaccac gttctttcag agcttcagca
    45421 gctttacaca gcgtaccgcc agtgtcgatc atatcatcga ccagtacgca gtcacgacct
    45481 gcaacgtcac cgatgatatg catcacctgg gaaacgttcg cacgcggacg acgtttgtcg
    45541 atgattgcca tatcggtatc gttcagcagc ttagcgatag cgcgggcacg cacaacgccg
    45601 ccgatgtccg gagaaaccac aattgggtta tccagattca gctgcagcat gtcttccagc
    45661 aggatcgggc taccaaatac gttatcaacc ggaacgtcga agaaaccctg aatctgttca
    45721 gcgtgcagat ccactgtcag cacacggtca acaccgacgc tggagaggaa gtctgcaacc
    45781 actttcgcag tgattggtac acgagcggaa cggacgcgac ggtcctggcg cgcatagcca
    45841 aagtagggga taacagcggt gatacgacct gcggaagcac gacgcagggc atcaaccata
    45901 acgactaatt ccatcaggtt gtcgttagta ggggcacaag tggactggat gatgaaaata
    45961 tcaccaccgc gtacattttc attaatttgt acgctgactt cgccatcgct aaagcgacct
    46021 acagcggcgt cgccgagtga agtgtacagg cggttggcaa tacgttgtgc tagttccggg
    46081 gtggcgttac cagcaaaaag cttcatatca ggcacgagaa gaacctcagg catgcgtcca
    46141 ttggtggaaa gaatctgccg aaaactgtgc gggccaggca tgatcctttc caggcggtgt
    46201 attaaagagc gcgatgcaac gtctggaaca aggtgacgtt gtcaccgaaa ctcagcttgc
    46261 ccggcttaaa gcatggctct gtgcaatggg gaaagattaa cgcctttcgc cacaaagcca
    46321 ttgagccatt ccggggcttg ctctagcacc tggcgggctt cagactctgt atcaaattca
    46381 gcaaagacac aggcccctgt cccagtcagg cgcgacgggg cgtattctaa cagccaggaa
    46441 agcaccgcat caacctcgcg aaaacgtttt cttgcgataa cctcgcaatc attgctgaat
    46501 tcacatttta gcaacgtttc tattgacctt tttggcgtat tgcgcgggag ttcaggatct
    46561 ttaaaaatca ccggagtcgg aatacttaca ccagggtgcg ccaccagata ccacttctct
    46621 ggcggatcca ccggcgttag tatttcacca acgccttcgg caaacgcggc atgcccccga
    46681 acaaagacag gaacatctgc gcccagcgtc agccccattt ccgccagctc atccatgctt
    46741 agcccgcatt gccagagatg atttaatgcc accaggaccg tcgcggcatt ggatgaacca
    46801 ccgccgagac cgccgcccat cggcaaacgc ttgtcaatgc tgatattcgc accgcttccc
    46861 gtcggaagac gcccgctgtc tgccgcagtt ttcatcaaca atcgcgctgc gcgaacgatc
    46921 aggttatctt catgttccac gccttcaacg ggcgttaaca gacgaatatc cccatcgtca
    46981 cgaagctcaa tgctgatggt gtcgccgtaa tcaagaaact gaaacagcgt ttgcagcgtg
    47041 tggtaaccat ccgcacgctg accggtaatg tataaaaaca gattaagttt tgccggagag
    47101 ggccactgtg tccgcattat ttcactatcc agttatccat ttttaacttg atgcgttgac
    47161 caccgtcggt gagttccata ttggctggca tcgcaggttg cgttttggtg tcataaccac
    47221 cataaacaac cttccagttt ttgccattct ggctgtaggt aatttcgctc aggcggtact
    47281 ggtcgtccag tttgtagtcg gttgcatcac ccggtaaacc taaaatccac tggcgcaagc
    47341 tgttgagcgg aattggcatt ccggtcaatt tgccaatcat ctcttcggcg tcatcggcgg
    47401 tataacgctg acctttattg tcgactaact gcacgttacc cggttgagca ttcagctcca
    47461 gttccgtgct gcccaatggg ttagtgagca gcagacggta gcgatcctgg ccggtttgct
    47521 gccagaaaaa gcgggcgtac actttttgtt ggtcagaaat ataagcgaac gcgccgcgag
    47581 tctgatactg attaagattg cgcacgtctt gctgatgctg acgccattgt ggcgaatccg
    47641 ggcttttgcc aggacctttg ggcgtggtaa cggaacaggc agtgagcaca agagcagcca
    47701 gcggtagcag gcggataaga cgaaaatcgg gcaggggcat agtgatgaca agtccttgag
    47761 atacgttgca gttataaccc ttaatgctag cgttaccgtc cgctatcgtc tatgttcaag
    47821 ttgtcttaat tgccagaatc taacggcttt cggcaattac tccaaaaggg ggcgctctct
    47881 tttattgatc ttacgcatcc tgtatgatgc aagcagacta accctatcaa cgttggtatt
    47941 atttcccgca gacatgaccc ttttagcact cggtatcaac cataaaacgg cacctgtatc
    48001 gctgcgagaa cgtgtatcgt tttcgccgga taagctcgat caggcgcttg acagcctgct
    48061 tgcgcagccg atggtgcagg gcggcgtggt gctgtcgacg tgcaaccgca cggaacttta
    48121 tcttagcgtt gaagagcagg ataacctgca agaggcgtta atccgctggc tttgcgatta
    48181 tcacaatctt aatgaagaag atctgcgtaa aagcctctac tggcatcagg ataacgacgc
    48241 ggttagccat ttaatgcgtg ttgccagcgg cctggattca ctggttcttg gggagccgca
    48301 gatcctcggt caggttaaaa aagcgtttgc cgattcgcaa aaaggtcata tgaaggccag
    48361 cgaactggaa cgcatgttcc agaaatcttt ctctgtagcg aaacgcgttc gcactgaaac
    48421 agatatcggt gccagtgctg tgtctgtcgc ttttgcggct tgtacgctgg cgcggcagat
    48481 ctttgaatcg ctctctacgg tcacagtgtt gctggtaggc gcgggcgaaa ccatcgagct
    48541 ggtagcgcgt catctgcgcg aacataaagt acagaagatg attatcgcca accgcactcg
    48601 cgaacgtgcc caaattctgg cagatgaagt tggcgcggaa gtgattgccc tgagtgagat
    48661 cgacgaacgt ctgcgcgaag ccgatatcat catcagttcc accgccagcc cgttaccgat
    48721 tatcgggaaa ggcatggtgg agcgcgcatt aaaaagtcgt cgtaaccaac caatgctgtt
    48781 ggtggatatt gccgttccgc gcgatgttga gccggaagtt ggcaaactgg cgaatgctta
    48841 tctttatagt gtggacgatc tgcaaagcat catttcgcac aacctggcgc agcgtaaagc
    48901 cgcagcggtt gaggcggaaa ctattgtcgc tcaggaaacc agcgaattta tggcgtggct
    48961 gcgagcacaa agcgccagcg aaaccattcg cgagtatcgc agccaggcag agcacgttcg
    49021 cgatgagtta accgccaaag cgttagcggc ccttgagcag ggcggcgacg cgcaagccat
    49081 tatgcaggat ctggcatgga aactgactaa ccgcttgatc catgcgccaa cgaaatcact
    49141 tcaacaggcc gcccgtgacg gggataacga acgcctgaat attctgcgcg acagcctcgg
    49201 gctggagtag cagtacatca ttttcttttt ttacagggtg catttacgcc tatgaagcct
    49261 tctatcgttg ccaaactgga agccctgcat gaacgccatg aagaagttca ggcgttgctg
    49321 ggtgacgcgc aaactatcgc cgaccaggaa cgttttcgcg cattatcacg cgaatatgcg
    49381 cagttaagtg atgtttcgcg ctgttttacc gactggcaac aggttcagga agatatcgaa
    49441 accgcacaga tgatgctcga tgatcctgaa atgcgtgaga tggcgcagga tgaactgcgc
    49501 gaagctaaag aaaaaagcga gcaactggaa cagcaattac aggttctgtt actgccaaaa
    49561 gatcctgatg acgaacgtaa cgccttcctc gaagtccgag ccggaaccgg cggcgacgaa
    49621 gcggcgctgt tcgcaggcga tctgttccgt atgtacagcc gttacgccga agcccgccgc
    49681 tggcgggtag aaatcatgag cgccagcgag ggtgaacatg gtggttataa agagatcatc
    49741 gccaaaatta gcggtgatgg tgtgtatggt cgtctgaaat ttgaatctgg cggtcatcgc
    49801 gtgcagcgtg ttcctgctac ggaatcgcag ggtcgtattc atacttctgc ttgtaccgtt
    49861 gcggtaatgc cagaactgcc tgacgcagaa ctgccggaca tcaacccagt agatttacgc
    49921 attgatactt tccgctcgtc aggggcgggt ggtcagcacg ttaacaccac cgattcggca
    49981 attcgtatta ctcacttgcc gaccgggatt gttgttgaat gtcaggatga acgttcacaa
    50041 cataaaaaca aagcgaaggc gctttcagtg ctcggtgctc gcatccacgc tgctgaaatg
    50101 gcaaaacgcc aacaggccga agcgtctacc cgtcgtaacc tgctggggag tggcgatcgc
    50161 agcgaccgta accgtactta caatttcccg caggggcgcg ttaccgatca ccgcatcaac
    50221 ctgacgctct accgcctgga tgaagtgatg gaaggtaagc tggatatgct gattgaaccg
    50281 attatccagg aacatcaggc cgaccaactg gcggcgttgt ccgagcagga ataatggaat
    50341 atcaacactg gttacgtgaa gcaataagcc aacttcaggc gagcgaaagc ccgcggcgtg
    50401 atgctgaaat cctgctggag catgttaccg gcagagggcg tacttttatt ctcgcctttg
    50461 gtgaaacgca gctgactgac gaacaatgtc agcaacttga tgcgctactg acacgtcgtc
    50521 gcgatggtga acccattgct catttaaccg gggtgcgaga attctggtcg ttgccgttat
    50581 ttgtttcgcc agcgacctta attccgcgcc cggatacgga gtgtctggtg gagcaggcac
    50641 tggcgcggtt gcctgaacaa ccttgccgta ttcttgatct cgggacgggt accggggcga
    50701 ttgcgcttgc gctggctagc gagcgcccgg actgcgaaat tatcgctgta gatcgtatgc
    50761 ctgatgctgt ctccctggca caacgtaatg cccagcatct ggcgatcaaa aatatccaca
    50821 ttctgcaaag cgactggttt agcgcgctag ccgggcagca gtttgcgatg attgtcagca
    50881 atccgccgta tattgacgag caggacccac atcttcaaca aggcgatgtc cgctttgagc
    50941 cgctcactgc gctggttgcg gcagacagtg gaatggcaga catcgtgcat atcatcgaac
    51001 agtcgcgtaa cgcgctggta tccggcggct ttctgcttct ggaacatggc tggcagcagg
    51061 gcgaagcggt gcgacaagca tttatcctcg cggggtatca tgacgtcgaa acctgccgtg
    51121 actatggtga taacgagcgc gtaacgctcg gccgctatta tcaatgacaa gtttttctac
    51181 actgcttagt gttcatctta ttagtatcgc gctttctgtt gggctattaa ccttacgttt
    51241 ctggctacgt tatcagaagc atccacaggc atttgctcgc tggacgcgca ttgtgccgcc
    51301 ggttgtcgat acggtgttat tgttaagcgg cattgcgttg atggctaaag cgcacatcct
    51361 gccattttcc gggcaggcac agtggctgac tgaaaagctg tttggagtta tcatttatat
    51421 cgttttgggt tttattgcac tcgattatcg tcgtatgcac agtcagcagg cgcgcattat
    51481 tgccttcccg ctggcgttgg tggtgctgta catcatcatt aaactcgcca ccacaaaagt
    51541 accgttactg gggtaagtca tgagatcgtt agctgatttc gaatttaata aagcgccatt
    51601 gtgcgaaggc atgatcctgg cttgcgaagc aatccgccgc gattttccct cgcaagatgt
    51661 ttacgacgaa ctggagcgtc tcgttagtct ggcgaaggaa gaaatcagcc agcttctgcc
    51721 tttagaagag cagctggaaa aactgatcgc gctgttttac ggcgactggg gatttaaagc
    51781 ctcacgcggc gtttatcgtc tttccgatgc attatggctg gaccaggtgt taaagaatcg
    51841 acagggcagt gcggtatcat taggtgcggt tttattatgg gtcgcgaatc gtctcgattt
    51901 gccgctgctg ccggtgattt tccctacgca gctgatattg cgcattgaat gtccggatgg
    51961 cgaaatttgg ctgattaatc cttttaacgg tgaatcgtta agcgaacata tgctggacgt
    52021 atggttaaag ggaaatatca gcccgtcggc ggaactgttt tatgaagacc ttgatgaagc
    52081 tgataacatt gaggtaatcc gcaaattgct ggatacactc aaagcctcgt tgatggaaga
    52141 aaatcagatg gagctggcgt tacgcaccag cgaagcttta ttacaattca accctgaaga
    52201 tccctatgaa attcgcgatc gcgggttgat ttatgcacaa ctggattgcg aacacgttgc
    52261 gttgaacgat ttaagttatt tcgttgaaca gtgtccggaa gacccgatca gcgaaatgat
    52321 ccgtgcgcaa ataaataaca tcgcgcataa acatattgtg ctgcattaat taatcgacat
    52381 tttactcaag attaaggcga tcctatgaaa caaaaagtgg ttagcattgg cgacatcaac
    52441 gtagcaaatg acctgccgtt cgtactgttt ggcggtatga acgtgctgga atctcgcgat
    52501 ctggcgatgc gcatttgcga gcactacgta actgtgaccc agaaactggg tatcccttac
    52561 gtgttcaaag cctcttttga caaagccaac cgctcctcca tccactctta tcgtggaccg
    52621 ggcctggaag aagggatgaa aatcttccag gagttgaagc agacttttgg cgtgaaaatt
    52681 atcaccgacg ttcacgaacc aagtcaggca cagcccgttg ctgatgtcgt ggatgtgatt
    52741 cagttgccgg cgtttcttgc tcgccagact gacctggttg aagccatggc gaaaaccggt
    52801 gcggtaatta acgtcaagaa accacagttt gtcagcccgg gacagatggg taatatcgtt
    52861 gataaattca aagaaggcgg caacgaaaaa gtgattcttt gcgatcgtgg tgctaacttc
    52921 ggctatgaca acctggttgt cgatatgctg ggcttcagca ttatgaagaa agtgtctggt
    52981 aactcgccgg tgattttcga cgtgacccac gcactgcaat gccgcgatcc gtttggcgca
    53041 gcttccggtg gtcgtcgtgc tcaggtggct gagctggcac gagccggtat ggcggtaggt
    53101 ctggcggggc tgtttattga agcgcatccg gatccggaac atgcgaaatg tgatggtcca
    53161 tccgcgctgc cgctggctaa actggaaccg ttcctcaagc agatgaaagc gattgatgat
    53221 ctggtgaaag gtttcgaaga actggatacc agcaagtaat cttttttgct tgaaaaataa
    53281 agtcttagcg ttctgcgtta agactttttt catgggtgcc agatacaaaa aaggccgcag
    53341 gctgttaccc ctgcggccgg tttcgggcgc atattgccat cacggcagcc tgacgcccgt
    53401 tttcacctta cttccggtta cgccaccagc cgacaatcgc tgcggtaata atccccgcca
    53461 ggatcggtgc tgccaggtcg tgccagaaaa tcatggcaaa cttcgcgagc gtcatatagc
    53521 cgccttgttg taatgacaac attttgcggc tattcttgaa ttgttgatgg ttcaagatta
    53581 gcccccgttc tgttgtcagg ttttacctct caacgtgcgg gggttttctc tttccagcaa
    53641 ccaatgccac cagggataaa gcccccgcaa cattgcgcct caccggataa cgccggcttg
    53701 gtgtggatac tacgactcaa ttcatcttca cttcatccct gaaatgtttg caatgaagag
    53761 tgcattccgg tttttcaaca gctgttacag tcgtttcatg agtgctctgg atgatgcttc
    53821 cagctcgggt tgccaatatt tacttgtgga agagataaag acaaaaatgg ccgcaggctg
    53881 ttacccctgc ggccggtttc gggcgcatat tgccatcacg gcagcctgac gcccgttttc
    53941 accttacttc cggttacgcc accagctgac aatcgctgcg gtaataatcc ccgccaggat
    54001 cggtgctgcc aggtcgtgcc agaaagtcat ggcaaactgc gcgagcgtca tatagccgcc
    54061 ttgttgtaat gacaacattt tgcggctatt cttgaagtgt ctggtttcaa gattagcccc
    54121 cgttttgttg tcaggtttta cctctcaacg tgcgggggtt ttctctttcc agcaaccaat
    54181 gccaccaggg ataaagcccc cgcaacattg cgcctcaccg gataattccg gcttggtgtg
    54241 gatactacgt ctcaattcat cttcacttca tccctgaaat gtttgcaata aagagtacat
    54301 tccggctttt caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag gctcgcaatt
    54361 ctgattaacg agctgtccag gtagtatgaa gaaatacaat aagcaggaat ttatcttatc
    54421 tcccacgatt tccgtcatcg gaggagatat aactgtcagg caaatatcgt catcaaatag
    54481 gcggcaaaca gtgccagatg cgctgcgcca ttgagcacgt tagtacgtcc ggtggagaag
    54541 gagatatggc acagcactaa agaggccacc atcaccacca tttctggcgc accaagtgca
    54601 aactgtaatt cgttacccgt cataaaggca attagcgtga cgacaggtac ggtaagcgaa
    54661 atggttgcta acactgaacc aaagaacaga ttcatcgcgc gctggacctg gttgttcaac
    54721 actgctttta acgcacctaa accttccggc gacagaatca acagagccac caggaagcca
    54781 gtaaaggcga caggggcatt catgctgtcg agcaatgtct ccagcgggct ggcattcatt
    54841 ttggtgactg caataacggc aatcagatgg ataatcaacc agatagcatg ccacacgctt
    54901 ctatgggcag acggtttacc gtgatgcggg tcgtcgtcat cactgtcatc ttcgtgctcg
    54961 tagacaaaca aactttgatg cgttttggtc tggatcagca aaaatacgcc atacattgcc
    55021 gcagaaatta atgctaccag taacgcctga ccggttgaaa aattcgccgc aggcagagcc
    55081 attggaaata ccagtacgat tatcgccagg gggaacagcg caattaaata ctgcttgata
    55141 ccaaacagat tcatatattg ggtggcaaac ttacgaccgc ccaacaataa tgaaaagcca
    55201 accagcccac cggtaacaat cataatgatt gaatagagcg tatcacgcat tagcgttggc
    55261 gcggcgtcgc cggttgccat taaagctgaa atcaaactga cttcaagaat aaccactgaa
    55321 aggctaagaa taagcgaacc gtaaggttct cccaggcgat gggctaacac gtccgcatga
    55381 cggacaacac taaaggcgct acttaaaata ccaataagcg caagaagatt gatggcaatg
    55441 accactggta gtgtctggct gcttccccac aggaacagca ctaccagcgc cagaaccggg
    55501 aaaataagcg aagtctcctt gtggcgggtt tttaccgcct cttgagcatt tgacattatg
    55561 gttatccctt tgcagatgaa tttatcgaaa atgtaaaaaa taggtaggaa aaataacaga
    55621 aagtgtctgg atatcggtaa cattttacga atttttaccc cactgtcgat tttttactca
    55681 ttgggacata aaaataagta ttacgtttag acaatgtttg ttttagcgtt tcttaagaac
    55741 agtctgacat gaaaattctt atgttttggc aagagtagat attgttgacc acacttaatg
    55801 ttcaactttg taaaaggagt caacgatgcc gtataaaacg aaaagcgatc tgccggaaag
    55861 cgtaaagcac gttctaccgt ctcatgccct ggatatctat aaagaagcgt tcaacagcgc
    55921 atgggatcaa tataaagata aagaagatcg gcgtgatgac gccagtcgcg aagaaaccgc
    55981 gcataaagtg gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg atgataaatg
    56041 gcataaaaaa tcgtaaaacc ggtcgcttag ttaaagctat tcgtgcggtg ttgccttgca
    56101 agtggtccgt ggattgcata ttgtcccgtt agtggtttca aaatgagcag taaaaatgtc
    56161 cggaagacac caaaaagttg tcgcagggaa gtatgcagtg gcggaggtgt aaggtgataa
    56221 cgcgtgattt cttgatgaat gccgattgta aaacggcatt tggtgccatt gaagaatcac
    56281 tcttatggtc agcagaacaa cgggcggctt cgctggcggc gacgctggct tgtcgacctg
    56341 atgagggacc ggtgtggatc ttcggctatg gatcgttgat gtggaatccg gcactggagt
    56401 ttaccgaatc gtgcaccggt acactggttg gatggcatcg cgcattttgc ctgcgcctga
    56461 ccgccgggcg cgggactgcg caccagccgg gacggatgct tgcactgaaa gagggcggac
    56521 gcaccacagg cgtcgcctat cgactgccag aagagacgct ggagcaggaa ctaaccctgt
    56581 tgtggaagcg agagatgatt accggctgtt atctgccaac ctggtgtcag cttgatcttg
    56641 atgatggacg cacagtaaac gccattgtgt ttattatgga tccgcgacat ccagaatatg
    56701 aatctgatac tcgcgctcag gtcatcgcgc cgttgattgc ggcggcgagc ggtccgctgg
    56761 gaaccaacgc acaatacctg ttttcactgg aacaggagct tatcaaactg ggaatgcagg
    56821 acgatgggct gaatgatttg ctggtatcgg taaaaaaact gctggcggag aattttccgg
    56881 atggtgtgtt acgtccggga ttcgcctgag taaacttccc gcatagtggg gcgtcagacg
    56941 ccccctcaaa cattaaaatg tgagcacttt atcggctgac agcgtccatt gcgccagttc
    57001 cacaagagta ccgatttcca ccccatcaat cagaggaagt gtactaatcc cgcgcccgtc
    57061 ggtacaggtt ttgcacaatt tcaccggtac attctgagcg gtaaggatct ccagcatttg
    57121 ctgaatgttg tagccttccc ctggtttttg cccgcgcaac ccggctgtga ccgcatcaga
    57181 catgaggaac agacgcagat ccagattgct ctcctgctct cgtaacgcaa tggccagccg
    57241 caagctgtta aacaaggatt cgctcccgta aggtgcgcca ttggcaacga tcacgatttt
    57301 ttgcattatt tactcctgta ttcagggaat tagacactca tcttctatct tactgcttct
    57361 gcagcgtctg accaatcggt cacattttta aggattttcc tgaaagcgcg agaaaatacg
    57421 acaaaagttg ccagtaatcg ttattcttta aggctatggt ttttcattat taccggaagt
    57481 taccgacgtt ttgagccgtt tcgttcctcg cattatcccg ttttatttac tcttgcttgt
    57541 agcaggcggt acagctaacg cacaatctac cttcgagcaa aaagcggcaa atccctttga
    57601 taataacaat gatggtctgc cggatttagg catggctccc gaaaatcatg atggggaaaa
    57661 acactttgct gaaattgtga aagatttcgg cgaaaccagt atgaatgata acgggctgga
    57721 tactggcgag caggcaaaag ctttcgcatt gggaaaagtc cgcgacgcgc ttagtcaaca
    57781 ggttaatcag cacgtagagt cctggctatc accgtgggga aatgccagtg ttgacgtcaa
    57841 agtggataac gaaggacatt tcaccggcag tcgtggaagc tggtttgtgc cgttacaaga
    57901 taatgatcgt tatctcacct ggagccagct tggtcttact cagcaggata atgggttggt
    57961 gagcaatgtg ggcgttgggc aacgctgggc gcgcggcaac tggctggtgg gttataacac
    58021 tttttatgac aacttgctgg acgaaaatct tcagcgagcg ggctttggtg ccgaagcgtg
    58081 gggcgaatat ttgcgattat cggcaaactt ttatcagccg tttgctgcat ggcatgaaca
    58141 gacagccacg caggaacaac ggatggcgcg cgggtacgac ctgacagctc ggatgcgcat
    58201 gccgttctat caacacctca ataccagtgt cagcctagaa cagtattttg gtgatcgtgt
    58261 tgatttgttt aactctggta cgggttatca caatcccgtc gcgttgagtc tgggattaaa
    58321 ttacacccct gtgccattag tcactgtgac ggcccagcat aaacagggtg aaagtggcga
    58381 aaatcaaaat aacctcgggc tgaatcttaa ttaccgcttt ggtgtaccgc tcaaaaaaca
    58441 actttctgcg ggcgaggttg ccgaaagtca gtcgttacgt ggtagtcgct atgacaatcc
    58501 gcagcgaaat aatctaccga ctcttgagta ccgacagcga aaaacgttaa cggtgtttct
    58561 ggcgacaccg ccgtgggatc taaaacctgg cgaaacagtg ccgctgaaat tacaaatccg
    58621 cagtcgttac ggtattcggc aactgatttg gcagggcgat acgcagatat taagtttgac
    58681 gccgggcgca caagccaaca gcgcggaggg ctggacgctg atcatgcctg actggcagaa
    58741 cggggaaggg gcaagcaatc actggcgatt gtctgtggtg gtggaagata accaggggca
    58801 gcgtgtctcc tccaatgaga tcacgctaac gcttgtcgaa ccgttcgatg cattgtcaaa
    58861 cgacgaactg cgctgggaac cgtaatcaga aaatgcgctc ctgatgcacc cataccgctg
    58921 cttccacgcg agacttgagc ttcattttct tcagcatgtg cttgacgtgc acttttactg
    58981 tgctttcggt gatatccagg cggcgggcaa tcatcttgtt cggcaaaccc tgggcaatca
    59041 gcttgagaat atcgcgctcg cgtggggtta actggttaac atcgcgctca gtagtggcac
    59101 ggttagcgcg caagctggcg gccagaacag gcgttaatgc ttcgcttaat accatttcgc
    59161 cagcagctgc ctgatgcaat gctttcagca gatcttccgg ttccatatct tttaacagat
    59221 agccatccgc gccgcgtttc agtgcggtga ccacatcttc ttcatggtta gagacgctga
    59281 ataccacaat gcgccctgag agggactttt cgcgcagttt atccagcgtt tccagaccgt
    59341 tcatgccggg catattgaga tctaacagga tcagatcggg atcaagagac tccgccagtt
    59401 caataccctg ttcgccatta ctcgcttcgc caaccacggt gatatctggt gccatactga
    59461 taagctgttt tacgccagtt cgcagcatcg ggtgatcgtc aatcagcagg atagtagccg
    59521 gttcctgatt actcatgggt atctccttgg acgtctgtga aagttttttc gggaataaag
    59581 gtgaccacca cttcggtgcc acctgattca cgacggcgga cgcggcaatc gcctcgtaaa
    59641 ctttgcgcac gatcgcgcat tattatcatg ccgtagtgat tgctgcggat ggcattttca
    59701 ggcacgccgc agccgttatc ctggactgtc agtttgacct gattatcgtt ttgcgccacc
    59761 gtcaccacga cttcactcgc ttgcgaatgt ttgagggcgt tacttaatgc ctcacgggca
    59821 atttgcaaca agtggattgc ctgatgcgaa ggcaccaggc gaggcggcaa ttgataatcc
    59881 agcttcaccg ggaagccaaa tttggcgctg tactcttcgc aactcgcctc cagcgccgga
    59941 cgtaatccag gctcggtgag ctgcaagcgg aatgtggtga gcaattcacg caactgcgcc
    60001 caggatgcat tcagttcgtt acggatctga cttaacagtt cgcggctgct ttctggcagc
    60061 gcatcgccct gcatctgtaa acaactcacc tgcatcttca tgcaagagag agattgggca
    60121 atagaatcat gcagttcgcg cgcaatggtg gcacgctctt ccatcacgat caactgttgc
    60181 tgacgttcct gatggcgatc cagtgccagc gtggcggtga gttgttcaac cagggtatcc
    60241 accagttgtt gttgatcatg gctaagatga cgcccctgcg gcagggtcgc cagcaaaata
    60301 ccgtactgcg tatgagagtc agccagccgc cacttcaggg tcgtgccgcg atcaccaacg
    60361 ggtaatacgc cgcgcgggca gagctggcag cctttatcat cacaagtcat atctggctgg
    60421 caggtaaact cctgatgatt ctcttcatca tcagtgtcat acacccgcaa ttcgatatca
    60481 cgtagcaggg ttaaattctg taagccgttg agtacaggtg acaggcgttc acacagcggg
    60541 gcgcgggaat gcaaacggcg gttagcctgc cataaaaaag agaggatctg atttttatgc
    60601 tccagcccgg cggttttctc ctgaacccgc tgctcaagta cggcataact ttcggccagt
    60661 tctgcagaca tattgttcaa cgcagttcca agcatcgcca tttcgttgcg cccgctgatg
    60721 tttgcgcgtt gggtaaaatc gcgatgactg acggcactcg ccattgccag cagttgccgc
    60781 cacggttgta gcagtcgcgc ccgcaaccag ataatagtga acaccagtaa aagtgccata
    60841 aataccgcca ttacccgatg gaccagtacc actgtctcga tgcgcatttc cgtggtgcgg
    60901 tcaaaaccag ataccagttg atcaagcccg gcaacaaact ggctgacatc cgctgacacc
    60961 gtttctcggt tttgtgcacg catcaacgca gggatcagtt cattacgcca gtaatcttgt
    61021 aaaccctgta attgcgccag ttgtccgtct cgttctgctg ctcgagtcaa ctcggcgcta
    61081 aatgccgttt gttccatctc tttaattaag ggcttgtctt tctcgcttaa tggcactgct
    61141 gccaacagac ggtaactttg catgcgcagc gatcccgctt tgttgatcgc atgggcgctg
    61201 ccctgaacgc cttgcaccag ccagccagaa accgccatcc ctgccagtcc aatagcagta
    61261 gaaagcaaca caataagcgc aacctgatta accagggtga gcggagagag acaacgttta
    61321 agcatgtaaa cctcttcctt caggctttaa atgagcaata accttaatga atgtgacgat
    61381 acattctgga atggcagtat tctcggctat tggctgaagt atacccatac ccggaaagag
    61441 ttactcctta tttgccgtgt ggttagtcgc tttacatcgg taagggtagg gattctacag
    61501 caccgtgaaa aatctcataa tttttatgaa atcactgtac tcactatggg taatgataaa
    61561 tatcaatgat agataaagtt atcttatcgt ttgatttaca tcaaattgcc tttagctcca
    61621 gacactaagg tggcagacat cgaaacgagt atcagaggtg tctatgagtc actcatctgc
    61681 ccccgaaagg gctactggag ctgtcataac agattggcga ccggaagatc ctgcgttctg
    61741 gcaacaacgc ggtcaacgta ttgccagccg caacctgtgg atttccgttc cctgtctgct
    61801 gctggcgttt tgcgtatgga tgttgttcag cgctgttgcg gtgaacttac cgaaagtcgg
    61861 ttttaatttt acgaccgatc agctattttt gttgactgcg ctgccttcgg tttctggcgc
    61921 gttattacgt gttccatact cctttatggt tcctatcttc ggtggtcgtc gctggacggc
    61981 gttcagcacc ggtattctga ttattccttg cgtctggctg ggttttgccg tgcaggatac
    62041 ctccacgcct tatagcgtct tcatcatcat ctctctgcta tgcggctttg ctggcgcgaa
    62101 cttcgcatcc agtatggcaa acatcagctt cttctttccg aaacagaagc agggtggcgc
    62161 gctggggctg aatggtggtc tgggaaacat gggcgtcagc gtcatgcagt tggttgctcc
    62221 gctggtggta tcactgtcga ttttcgcagt atttggtagc cagggcgtca aacagccgga
    62281 tgggactgag ctgtatctgg cgaatgcgtc ctggatatgg gtgccgttcc ttgccatctt
    62341 cactattgcg gcgtggtttg gcatgaacga tcttgctacc tcgaaagcct ccatcaagga
    62401 gcagttgccg gtactcaaac ggggtcatct gtggattatg agcctgctgt atctggcaac
    62461 cttcggctcc ttcatcggct tctccgcggg ctttgcgatg ctgtcaaaaa cgcagttccc
    62521 ggatgttcag attctgcaat acgctttctt cgggccgttt attggtgcgc tggcgcgttc
    62581 tgcaggtggt gcattatctg accgtctggg cggaactcgt gtcacgctgg tgaactttat
    62641 tctgatggcg attttcagcg gcctgctgtt cctgacctta ccgactgacg ggcagggcgg
    62701 aagcttcatg gcgttcttcg cggtcttcct ggcgctgttc ctgacagctg ggctgggtag
    62761 tggttccact ttccagatga tttcagtgat cttccgtaaa ctgacaatgg atcgcgtgaa
    62821 agcagaaggg ggttctgacg aacgtgcgat gcgtgaagcg gcaaccgaca cggcggcggc
    62881 gttgggtttc atctctgcga ttggcgcgat aggtggcttc tttatcccga aagcgttcgg
    62941 tagctcgctg gcattaacgg gttcgccagt tggcgcaatg aaagtatttt tgattttcta
    63001 tatcgcctgc gtagtgatta cctgggcggt atatggtcgg cattctaaaa aataaaccgt
    63061 tactcgtcat acttcgggtt acatgtgctg cggctgcgtt cattcacccc agtcacttac
    63121 tttagtaagc tcctgggatt cattcacttg ccgccttcct gtaaaccgaa ttatatagag
    63181 taaaatattt gattatcctt tgcgcggcat gatgtcgcgc tttttttatg cgttatttag
    63241 ttacaacata ctaatgttat atggcttatt tcgccggatt tcattaagag ccattaatat
    63301 gttacccatg gggaatactc cttaataccc atctgcataa aaatcttcat gctttaaata
    63361 actacaggta taaaacgtct taatttacag catgttatgc tttaaccgtt aattatccta
    63421 aaggggtatc ttaggaattt actttatttt tcatccccat cacacttgat cgttatcaat
    63481 tcccacgctg tttcagagcg ttaccttgcc cttaaacatt agcaatgtcg atttatcaga
    63541 gggccgacag gctcccacag gagaaaaccg atgagtaaat tcctggaccg gtttcgctac
    63601 ttcaaacaga agggtgaaac ctttgccgat gggcatggcc agcttctcaa taccaaccgt
    63661 gactgggagg atggatatcg ccagcgttgg cagcatgaca aaatcgtccg ctctacccac
    63721 ggggtaaact gcaccggctc ctgcagctgg aaaatctacg tcaaaaacgg tctggtcacc
    63781 tgggaaaccc agcagactga ctatccgcgt acccgtccgg atctgccaaa ccatgaacct
    63841 cgcggctgcc cgcgcggtgc cagctactcc tggtatcttt acagtgccaa ccgcctgaaa
    63901 tacccgatga tgcgcaaacg cctgatgaaa atgtggcgtg aagcgaaggc gctgcatagc
    63961 gatccggttg aggcatgggc ttctatcatt gaagacgccg ataaagcgaa aagctttaag
    64021 caggcgcgtg gacgcggtgg atttgttcgt tctttctggc aggaggtgaa cgaactgatc
    64081 gccgcatcta acgtttacac catcaaaaac tacggcccgg accgtgttgc tggtttctcg
    64141 ccaattccgg caatgtcgat ggtttcttac gcatcgggtg cacgctatct ctcgctgatt
    64201 ggcggtactt gcttaagctt ctacgactgg tactgcgacc tgcctcctgc gtctccgcaa
    64261 acttggggcg agcaaactga cgtaccggaa tctgctgact ggtataactc cagctacatc
    64321 atcgcctggg gttcaaacgt gccgcagacg cgtaccccgg atgctcactt ctttactgaa
    64381 gtgcgttaca aaggcaccaa aactgttgcc gtcacaccag actacgctga aatcgccaaa
    64441 ctgtgcgatc tgtggctggc accgaaacag ggcaccgatg cggcaatggc gctggcgatg
    64501 ggccacgtaa tgctgcgtga attccacctc gacaacccaa gccagtattt caccgactat
    64561 gtgcgtcgct acaccgacat gccgatgctg gtgatgctgg aagaacgcga cggttactac
    64621 gctgcaggtc gtatgttgcg cgctgctgat ctggttgatg cgctgggcca ggaaaacaat
    64681 ccggaatgga aaactgtcgc ctttaatacc aatggcgaaa tggttgcgcc gaacggttct
    64741 attggcttcc gctggggcga gaagggcaaa tggaatcttg agcagcgcga cggcaaaact
    64801 ggcgaagaaa ccgagctgca actgagcctg ctgggtagcc aggatgagat cgctgaggta
    64861 ggcttcccgt actttggtgg cgacggcacg gaacacttca acaaagtgga actggaaaac
    64921 gtgctgctgc acaaactgcc ggtgaaacgc ctgcaactgg ctgatggcag caccgccctg
    64981 gtgaccaccg tttatgatct gacgctggca aactacggtc tggaacgtgg cctgaacgac
    65041 gttaactgtg caaccagcta tgacgatgtg aaagcttata ccccggcctg ggccgagcag
    65101 attaccggcg tttctcgcag ccagattatt cgcatcgccc gtgaatttgc cgataatgct
    65161 gataaaacgc acggtcgttc gatgattatc gtcggtgcgg ggctgaacca ctggtatcac
    65221 ctcgatatga actatcgtgg tctgatcaac atgctgattt tctgcggctg tgtcggtcag
    65281 agcgggggcg gctgggcgca ctatgtaggt caggaaaaac tgcgtccgca aaccggctgg
    65341 cagccgctgg cgtttgccct tgactggcag cgtccggcgc gtcacatgaa cagcacttct
    65401 tatttctata accactccag ccagtggcgt tatgaaaccg tcacggcgga agagttgctg
    65461 tcaccgatgg cggacaaatc ccgctatacc ggacacttga tcgactttaa cgtccgtgcg
    65521 gaacgcatgg gctggctgcc gtctgcaccg cagttaggca ctaacccgct gactatcgct
    65581 ggcgaagcgg aaaaagccgg gatgaatccg gtggactata cggtgaaatc cctgaaagag
    65641 ggttccatcc gttttgcggc agaacaacca gaaaacggta aaaaccaccc gcgcaacctg
    65701 ttcatctggc gttctaacct gctcggttct tccggtaaag gtcatgagtt tatgctcaag
    65761 tacctgctgg ggacggagca cggtatccag ggtaaagatc tggggcaaca gggcggcgtg
    65821 aagccggaag aagtggactg gcaggacaat ggtctggaag gcaagctgga tctggtggtt
    65881 acgctggact tccgtctgtc gagcacctgt ctctattccg acatcatttt gccgacggcg
    65941 acctggtacg aaaaagacga catgaatact tcggatatgc atccgtttat tcacccgctg
    66001 tctgcggcgg tcgatccggc ctgggaagcg aaaagcgact gggaaatcta caaagccatc
    66061 gcgaagaaat tctccgaagt gtgcgtcggc catctgggta aagaaaccga catcgtcacg
    66121 ctgcctatcc agcatgactc tgccgctgaa ctggcgcagc cgctggatgt gaaagactgg
    66181 aaaaaaggcg agtgcgacct gatcccaggt aaaaccgcgc cacacattat ggtcgtagag
    66241 cgcgattatc cggcgactta cgaacgcttt acctctatcg gcccgctgat ggagaaaatc
    66301 ggtaatggcg gtaaagggat tgcctggaac acccagagcg agatggatct gctgcgtaag
    66361 ctcaactaca ccaaagcgga aggtccggcg aaaggccagc cgatgctgaa caccgcaatt
    66421 gatgcggcag agatgatcct gacactggca ccggaaacca acggtcaggt agccgtgaaa
    66481 gcttgggctg ccctgagcga atttaccggt cgtgaccata cgcatctggc gctgaataaa
    66541 gaagacgaga agatccgctt ccgcgatatt caggcacagc cgcgcaaaat tatctccagc
    66601 ccgacctggt ctggtctgga agatgaacac gtttcttaca acgccggtta caccaacgtt
    66661 cacgagctga tcccatggcg tacgctctct ggtcgtcagc aactgtatca ggatcaccag
    66721 tggatgcgtg atttcggtga aagcctgctg gtttatcgtc cgccgatcga cacccgttcg
    66781 gtgaaagaag tgataggcca gaaatccaac ggcaacccgg aaaaagcgct caacttcctg
    66841 acgccgcacc agaagtgggg tatccactcc acctacagcg acaacctgct gatgctgact
    66901 ttaggtcgcg gtggtccggt ggtctggttg agtgaagccg atgccaaaga tctgggtatc
    66961 gccgataacg actggattga agtattcaac agcaacggtg ctctgactgc ccgtgcggtt
    67021 gtcagccagc gtgttccggc agggatgacc atgatgtacc acgcgcagga acgtatcgtt
    67081 aacctgcctg gttcggaaat tacccaacag cgtggtggta tccataactc ggtcacccgt
    67141 atcacgccga aaccgacgca tatgatcggc ggctatgccc atctggcata cggctttaac
    67201 tactacggca ccgtaggttc taaccgcgat gagtttgttg tagtgcgtaa gatgaagaac
    67261 attgactggt tagatggcga aggcaatgac caggtacagg agagcgtaaa atgaagattc
    67321 gttcacaagt cggcatggtg ctgaatctcg ataagtgcat cggctgccac acctgttcag
    67381 ttacctgtaa aaacgtctgg accagccgtg aaggcgtgga atacgcgtgg ttcaacaacg
    67441 tggaaaccaa gccgggtcag ggcttcccga ctgactggga aaaccaggaa aaatacaaag
    67501 gcggctggat ccgtaaaatc aacggcaaac tgcagccgcg catgggtaac cgtgccatgt
    67561 tgctgggtaa aatcttcgct aacccgcatc tgccggggat cgacgattat tacgagccgt
    67621 tcgattttga ctatcagaac ctgcataccg cgccggaagg cagcaaatcg cagccgattg
    67681 cccgtccgcg ttcgctgatt accggggaac ggatggcgaa aatcgaaaaa gggccgaact
    67741 gggaagatga cctgggtggt gagtttgaca aactggcgaa agacaagaac ttcgacaaca
    67801 tccagaaggc gatgtatagc cagttcgaaa acacctttat gatgtatttg ccgcgcctgt
    67861 gcgaacactg cctgaacccg gcatgtgtgg cgacctgccc gagcggtgcg atttacaagc
    67921 gtgaagaaga tggcatcgtc ctgatcgacc aggataaatg ccgtggctgg cgtatgtgca
    67981 tcaccggatg cccgtacaaa aaaatctact tcaactggaa gagcggtaag tctgagaagt
    68041 gcatcttctg ctatccgcgt attgaagcgg gtcagccgac cgtgtgctca gaaacctgtg
    68101 tcggtcgtat ccgttatctt ggcgtgctgc tgtacgatgc cgacgcgatt gaacgtgcag
    68161 ccagcaccga gaacgagaaa gatctttacc agcgtcagct ggaggtgttc ctcgatccga
    68221 acgatccgaa agtcatcgag caggcgatta aagacggtat tccgctgagc gttattgaag
    68281 ccgcacagca gtcgccggtt tataaaatgg caatggaatg gaaactggcg ctgccgctgc
    68341 atccagaata tcgcacactg ccgatggtct ggtacgtgcc gcctctgtct ccgattcagt
    68401 ctgcagcaga cgcgggtgag ctgggtagca acggcattct gccagacgtc gaaagcttgc
    68461 gtattccggt acagtatctg gcgaatctgc tgaccgccgg tgataccaaa ccggtactgc
    68521 gcgcactgaa acgtatgctg gcgatgcgtc attacaaacg tgctgaagcc gttgacggta
    68581 aagttgatac ccgtgcgctg gaagaggtcg gtctgaccga agcccaggca caggagatgt
    68641 accgttatct ggcgattgct aactacgaag atcgctttgt ggtgccgagt agtcatcgtg
    68701 aactggcacg ggaagccttc ccggagaaaa atggctgcgg ctttaccttt ggtgatggct
    68761 gccacggttc agataccaaa ttcaatctgt tcaacagccg tcgtatcgat gccatcgatg
    68821 tgaccagcaa aacggagccg catccatgat cgaactcgtg attgtatcgc gtctccttga
    68881 atatccggat gctgccttat ggcagcatca acaagagatg tttgaggcga ttgctgcgtc
    68941 gaaaaatctg tcgaaagaag atgcccatgc gctgggcatt ttcctgcgcg atttaacggc
    69001 gatggatccg ctcgatgccc aggcccagta cagcgaactg ttcgaccgtg gccgcgccac
    69061 gtcactgttg ctatttgaac atgttcacgg tgaatcccgt gaccgcggcc aggcgatggt
    69121 ggacctgctg gcgcagtacg agcagcacgg cttgcagtta aacagccgcg aattaccgga
    69181 ccatctgccg ctgtatctgg agtacctgtc gcagctgccg caaagcgaag ccgtggaagg
    69241 tttgaaagat atcgcgccga ttctggcatt gctgagcgcg cgtctgcaac agcgtgaaag
    69301 ccgttatgcc gtgatgtttg atctgctgct gaaattggcc aataccgcta tcgacagcga
    69361 caaagtggcg gaaaaaattg ccgacgaagc gcgcgatgat acgccgcagg cgctggatgc
    69421 tgtctgggaa gaagagcagg ttaaattctt tgctgacaaa ggctgcggtg attcagcaat
    69481 cactgctcat cagcgtcgct ttgccggtgc cgtcgcgccg caatatctga atatcaccac
    69541 cggaggacag cactaatgca attcctgaat atgttcttct ttgatatcta cccgtacata
    69601 gccggggcgg tcttcctgat tggtagctgg ctgcgttatg actacgggca gtacacctgg
    69661 cgcgcggcgt ccagccagat gctggatcgc aaagggatga acctggcgtc gaacctgttc
    69721 catatcggta ttctggggat ttttgttggt cacttcttcg gtatgctgac gccgcactgg
    69781 atgtatgaag cctggctgcc gattgaagtg aaacagaaaa tggcgatgtt tgctggtggt
    69841 gccagcggcg tgctgtgtct gattggcggc gtgctgttgc tgaagcgtcg tctgttcagc
    69901 ccacgcgtgc gtgcaaccac taccggagcg gatatcctga tcctgtcgct gctcgttatc
    69961 cagtgcgcgc tgggcctgtt gaccattccg ttctccgctc agcatatgga cggtagcgag
    70021 atgatgaaac tggttggctg ggcgcagtcg gtggtgacct tccacggtgg cgcttctcaa
    70081 cacctcgatg gtgtggcgtt tatcttccgt ctgcacctgg tgctggggat gacgttattc
    70141 ctgctgttcc cgttctcgcg tctggtacac atctggagcg taccggtgga gtatctgaca
    70201 cgtaagtacc agctggtgcg cgctcgtcac taagcgaatt ttagttcaca tagaccctgc
    70261 ttcggcgggg tttttttatg ggcacggtgc ggggtgagtt gtcggatgcg cttcgcttat
    70321 ccgacctaca ggggaggata ttgtaggccc ggtaaacgtg gtgccatcgg gcaaaattag
    70381 ctcggagtaa caggttttga ttatttaagg aagcgatggt ggtgggggaa ggattactca
    70441 gcgctgcgcg cttcgccctt cgggtcgttg cctgcggcaa cgctctctcg ctggcgctca
    70501 agtcgaacct tggtcgaagc ttctcatcct tccccgcttg ggcagaatat ttaattgcgg
    70561 attcgtttga gagtttacgg acttttgaaa agtgatggtg gtgggggaag gattcgaacc
    70621 ttcgaagtcg atgacggcag atttacagtc tgctcccttt ggccgctcgg gaaccccacc
    70681 acggggtaat gctttttact ggcctgctcc cttatcggga agcggggcgc atcatatcaa
    70741 atgacgcgcc gctgtaaagt gttacgttga gaaaaatgaa ctggttgcgt aattttcatc
    70801 cgtaacggat taaaggtaac cagttatttt tgctggcgat taaagaataa tcgtccgatt
    70861 accgtaaaca aagacgcgct gtgccagtac tttgtatagc gcacgactta agacgttttt
    70921 ctcgacgtca cgacctgcgc gcatcatatc ttcagctgtg taggtatgat cgacatgaat
    70981 aacgtcctgc atgatgattg ggccttcgtc cagattgtca ttcacatagt gagcggttgc
    71041 gccaataatc ttcacaccac gttcataggc ctggtgataa ggacgtgcgc cgataaacgc
    71101 aggcaggaat gaatggtgaa tattgatgat tttattcggg aagcgtgcca caaattccgg
    71161 cgttaatacc cgcatatact tcgccagcac cacatagtca ggttgataag catcaatggc
    71221 gtccgccatc ttttgatcgt gctcgttgcg gcttaaccct tcatggctta ccagctcaaa
    71281 cggaatatca aaacgctcaa ccagagaacg taaagtatcg tggttaccaa taactgccgc
    71341 gatttcgaca tccaggccgc cgtaattggc tttcatcaac aaatcgccaa ggcaatgcgc
    71401 ttctttagtg accagaatca ctatccggcg acgaccggca ggattcagct cacgcacgga
    71461 gccttctggc aatgcgctat cgagatccgc cagcagggtg gaatcattaa aaatcccttc
    71521 cagttccgtg cgcataaaaa agcgcccggt acggtgatca acaaattcat tgttctgtac
    71581 gatatttaac tcgtgcttgt agcaaatatt ggtaatgcgt gcgatcagac ctttttggtc
    71641 cggacaaata gtacgcagaa ctttacgttg gagtgaatgc attgctggaa aaaccttgtt
    71701 gagagtgttt gctaaaccgt accgtcaacc attattggcc gcagcacttt ttaaattttt
    71761 tacctgaacc acaagggcag ggatcgttgc gaccaaactg cggacgtgta ccgtcaatat
    71821 agtaccattg accgttttct ttcaaaaatc gcgaacgttc aattattgca cctgttttac
    71881 caccttcggt aaatcgagca acaaaactga caaatccgat attgtcggcg tcctgccaac
    71941 aatgttcaaa tacggtcaat cccaaccatt cagtatgcgc aaatccggcc atcaattcgg
    72001 cacgtaacgc cgctgctcca caagagggat gccaggtctt tattaaataa tctgcgtctt
    72061 gcatcacaaa agcgcagtaa cgcgaacgca tgagatgttc tggatcaggt gcaacctttt
    72121 caccagacac ataagggtgg caacataggc tatactcgac agcactacca cagggacaaa
    72181 gctgagacac aaataatctc cctggaaaca ataacggcgt attaaccgcc tgagtagcac
    72241 tatgttaacc gagcagtagc gatgtggcta cgattgcatt ccaggggaat cttgcgggaa
    72301 taatgagaaa gataaaaata gggctggcgc tgggatctgg cgcggcgaga ggttggtcgc
    72361 atattggcgt tattaatgcg ctaaaaaaag tgggtattga aattgatatc gttgcaggat
    72421 gttcaattgg ttcgctggtg ggcgctgcct atgcatgcga tcgattatct gcgctggaag
    72481 attgggtgac ctctttcagt tattgggatg ttttacgcct gatggatctc tcctggcagc
    72541 gcggtgggtt actgcgcggc gagcgtgtct tcaatcaata ccgcgaaata atgccggaaa
    72601 cagagatcga aaattgttcc cgtcgctttg cggctgttgc caccaattta agtacgggac
    72661 gtgaattatg gtttactgaa ggcgatctcc atcttgctat tcgcgcatca tgcagtattc
    72721 caggactcat ggcacctgtt gcacataacg gctactggct ggttgatgga gcagtcgtta
    72781 acccaattcc tatttccctc acgcgtgcat tgggggctga tattgtgata gcggttgacc
    72841 tgcagcacga tgctcatttg atgcaacaag atttgctctc ctttaatgtc agtgaagaaa
    72901 atagcgagaa tggtgattct ctgccgtggc atgcgcgtct gaaagaaagg ttaggcagca
    72961 taacgacacg tcgggcggtg acagcaccaa cggcaacaga gattatgacc acttctatcc
    73021 aggtgctgga gaaccgcctt aaaaggaacc gcatggcagg tgatccgccc gatattctga
    73081 ttcaacctgt ttgcccgcaa atatctacgc ttgatttcca tcgcgcgcac gctgccattg
    73141 cggccggaca gctggcagtg gaaaggaaaa tggacgaact tttgccgttg gtacgcacca
    73201 acatttgacc agaattttta tctacactta agttaattct gacaggcgca ggtggcaata
    73261 gcatgccact attgagtaaa gccagtcagg ggagagaaca tgacgcagcc attggtcgga
    73321 aaacagattc tcattgttga agatgagcag gtatttcgct cgcttctgga ttcatggttt
    73381 tcctcattgg gagcgacaac ggtactggcg gctgatgggg tggatgccct tgagttgctg
    73441 ggaggtttca ctccagacct gatgatatgt gatatcgcga tgccacgaat gaacgggctt
    73501 aaactgctgg agcatatacg taacagaggc gaccagaccc cagttctggt gatatctgcc
    73561 actgaaaata tggcagatat tgccaaagcg ttacgtctgg gcgttgaaga tgttttgctg
    73621 aaaccagtta aagatctgaa tcgcttgcgc gagatggttt ttgcctgtct ctatcccagc
    73681 atgtttaatt cgcgcgttga ggaagaggaa aggctttttc gcgactggga tgcaatggtt
    73741 gataaccctg ccgcagcggc gaaattatta caggaactac aaccgccggt tcagcaggtg
    73801 atttcccatt gccgggttaa ttatcgtcaa ttggttgccg cggacaaacc cggcctggtg
    73861 cttgatattg ccgcactttc ggaaaacgat ctggcatttt attgccttga tgtcacccga
    73921 gctggacata atggcgtact tgctgccttg ttattacgcg cattgtttaa cggattatta
    73981 caggaacagc ttgcacacca aaatcaacgg ttgccagagt tgggcgcgtt attgaagcag
    74041 gtaaaccatt tactccgtca ggccaatctg ccggggcagt ttccgctatt agttggctat
    74101 tatcatcgcg aactgaaaaa tctcattctg gtttctgcgg gtctgaatgc gacgttaaat
    74161 accggcgaac accaggtgca aatcagtaat ggtgttccgt taggcacttt aggtaacgct
    74221 tatttgaatc aattgagcca gcgatgcgat gcctggcaat gccaaatatg gggaaccggt
    74281 ggtcgactgc gcttgatgtt gtctgcagaa tgagcaaacg ataacgcggg ctaaatttgc
    74341 attacctgct aatgtcggct ggtggtacta tcgtcgccat tcgtataagt aattgtctta
    74401 attatgctaa ctcgcctcct tttcagaact tagccccttc ggggtgctga tatactggga
    74461 tgcgatacag aaatatgaac acgttcaaaa cacgaacagt ccaggagaat ttaaatggct
    74521 gccattaata cgaaagtcaa aaaagccgtt atccccgttg cgggattagg aaccaggatg
    74581 ttgccggcga cgaaagccat cccgaaagag atgctgccac ttgtcgataa gccattaatt
    74641 caatacgtcg tgaatgaatg tattgctgct ggcattactg aaattgtgct ggttacacac
    74701 tcatctaaaa actctattga aaaccacttt gataccagtt ttgaactgga agcaatgctg
    74761 gaaaaacgtg taaaacgtca actgcttgat gaagtgcagt ctatttgtcc accgcacgtg
    74821 actattatgc aagttcgtca gggtctggcg aaaggcctgg gacacgcggt attgtgtgct
    74881 cacccggtag tgggtgatga accggtagct gttattttgc ctgatgttat tctggatgaa
    74941 tatgaatccg atttgtcaca ggataacctg gcagagatga tccgccgctt tgatgaaacg
    75001 ggtcatagcc agatcatggt tgaaccggtt gctgatgtga ccgcatatgg cgttgtggat
    75061 tgcaaaggcg ttgaattagc gccgggtgaa agcgtaccga tggttggtgt ggtagaaaaa
    75121 ccgaaagcgg atgttgcgcc gtctaatctc gctattgtgg gtcgttacgt acttagcgcg
    75181 gatatttggc cgttgctggc aaaaacccct ccgggagctg gtgatgaaat tcagctcacc
    75241 gacgcaattg atatgctgat cgaaaaagaa acggtggaag cctatcatat gaaagggaag
    75301 agccatgact gcggtaataa attaggttac atgcaggcct tcgttgaata cggtattcgt
    75361 cataacaccc ttggcacgga atttaaagcc tggcttgaag aagagatggg cattaagaag
    75421 taacatccgt atcggtgtta tccacgaaac ggcgttgagc aatcgacgcc gtttttttat
    75481 agcttattct tattaaattg tcttaaaccg gacaataaaa aatcccgccg ctggcgggat
    75541 tttaagcaag tgcaatctac aaaagattat tgcttgatca ggaaatcgtc gagggattta
    75601 ccttgctcat ccattgcttt tttgattaca gctggagtac ggccttggcc agtccaggtt
    75661 ttagtttcgc cgttttcgtc aacgtagcta tattttgccg gacgctgagc acgtttagct
    75721 ttggtgccag atttaacggc agcaaggcta ttcagcagtt cgttcgggtc aataccgtca
    75781 gcgatcagca tttcgcgata ttgctgcagc ttacgagtgc gctcttcaac ttcagcagca
    75841 gccgcgcttt cttcttcgcg acgttcgtta acaacaactt ctaatttttc cagcatttct
    75901 tccagcgttt caagtgtaca ttctcttgcc tgcgcacgaa gagtacggat gttgttcaga
    75961 attttaagtg cttcgctcat tgtagtaatc tcaaacttat attggggtgg tttgttgagg
    76021 taataataga gccttaaatt cagttgtgca atagccagga atgtaaggaa ttcaaaattg
    76081 ttctttattt tgtgccgcca ataaatatct tttcataaaa ttagccagaa aagacgcggc
    76141 atatagccct atttacaccg atgatttcgc agcacgtgag gttaaaactt cctgattcat
    76201 gtcacatttt atggggagat tatcgtaggc tgacgacctt tcagtcttct gtattagttg
    76261 tgtttacgag aattccctat taagcgaatg atgaaaagta gaacagtcgc aataagagca
    76321 tggacttagt attgcactat ctcctggagg tcaacagagg gctattactt gcgcaacagg
    76381 ttaaagattg tgaatagtta ccagcagtca tttacccgct tataacaagc gaggcagttg
    76441 taatgatagc tcagaaggat tatgcaaggc ttcgtaaggg agaacgcata tacccacttc
    76501 tgtgcatact gttgagctga aaaactgacg aattatgata aactccagcc aactttattt
    76561 catatcattg agggcctgtg gctgatggca cagctatatt tctactattc cgcaatgaat
    76621 gcgggtaagt ctacagcatt gttgcaatct tcatacaatt accaggaacg cggcatgcgc
    76681 actgtcgtat atacggcaga aattgatgat cgctttggtg ccgggaaagt cagttcgcgt
    76741 ataggtttgt catcgccggc aaaattattt aaccaaaatt catcattatt tgatgagatt
    76801 cgtgcggaac atgaacagca ggcaattcat tgcgtactgg ttgatgaatg ccagttttta
    76861 accagacaac aagtatatga attatcggag gttgtcgatc aactcgatat acccgtactt
    76921 tgttatggtt tacgtaccga ttttcgaggt gaattattta ttggcagcca atacttactg
    76981 gcatggtccg acaaactggt tgaattaaaa accatctgtt tttgtggccg taaagcaagc
    77041 atggtgctgc gtcttgatca agcaggcaga ccttataacg aaggtgagca ggtggtaatt
    77101 ggtggtaatg aacgatacgt ttctgtatgc cgtaaacact ataaagaggc gttacaagtc
    77161 ggctcattaa cggctattca ggaaaggcat cgccacgatt aataagaatt tctttactga
    77221 cagggtgagc agggcacttt tatcctgtca gttcgtttta cgcacttctt ccgggctata
    77281 tacccttctc ggcagttttt taacgccgct atacgcctca cagggctctt aagcaccgac
    77341 gttgacttgt gacctgtaaa gtacaatatc cctgtgttta ggcgttatac atcgtcgcaa
    77401 atatgatgaa ggctaatgct gtcggtttat ggaaaagttg ctttgggtaa acaaaaaaac
    77461 ggccccagaa ggggccgttt atattgccag acagcgctac tgattaagcg gattttttcg
    77521 cttttttctc agctttagcc ggagcggctt cttttttcgc tgcagtttca ccttctacat
    77581 aatcacgacc gtagtaggta tccagcagga tctgtttcag ctcggagatc agcgggtaac
    77641 gcgggttagc gccggtgcac tggtcatcga atgcatcttc agacagttta tccacgttcg
    77701 ccaggaagtc tgcttcctga acgccagctt cacggataga tttcggaata cccagttcag
    77761 ctttcagcgt ttccagccat gccagcagtt tctcgatctt agcagcagta cggtcgcccg
    77821 gtgcgctcag acccaagtgg tcggcaattt cagcataacg acggcgagcc tgcggacggt
    77881 catactggct gaatgcagtc tgcttggtcg ggttgtcgtt cgcattgtag cgaataacgt
    77941 tacaaatcag cagggcgttt gccagaccgt gcggaatatg gaactgggaa cccagtttgt
    78001 gcgccattga gtgacataca cccaggaagg cgttcgcaaa cgcgataccc gcgatagtcg
    78061 ctgcactgtg aacacgttca cgcgctaccg gatttttaga cccttcgtgg taggacgctg
    78121 gcagatattc tttcagcagt ttcagtgcct gcagagcctg accatcagag aactcagatg
    78181 ccagtacaga aacataagct tccatggcgt gagttactgc gtccagacca ccgaaagcac
    78241 acagggactt cggcatgtcc ataaccaggt tggcgtcgac aatcgccata tccggagtca
    78301 gcgcatagtc tgccagcgga tatttctgac cagtagcgtc gtcagttaca accgcaaacg
    78361 gagtgacttc agaacctgta ccagaagtgg tggtgacagc gatcattttc gctttcacgc
    78421 ccattttcgg gaacttgtag atacgtttac ggatatccat aaagcgcagc gccagctctt
    78481 cgaagtgagt ttccggatgt tcgtacataa cccacatgat cttcgcggcg tccatcgggg
    78541 aaccaccacc cagcgcgata atcacgtctg gtttgaagga gtttgccagt tctgcacctt
    78601 tacgaacgat gctcagggtc gggtccgctt ctacttcgaa gaagacttca gtttcaacgc
    78661 ctgctgcttt cagtacggaa gtgatctgat cagcataacc attgttgaac aggaagcggt
    78721 cagtcacgat gagcgcacgt ttgtggccat cagtaatcac ttcatccagc gcgattggca
    78781 gggagccacg gcggaagtag atagatttcg gaagtttgtg ccacaacatg ttttcagctc
    78841 gcttagcaac ggttttcttg ttgatcaggt gtttcggacc aacgttttca gagatggagt
    78901 taccacccca ggaaccacaa cccagagtca gggaaggtgc gagtttgaag ttatacaggt
    78961 caccgatacc accctgagac gctggggtgt taatcaggat acgagccgtt ttcattttct
    79021 gaccgaagta agaaacgcga gccggttggt tatcctggtc agtgtacagg caagaggtat
    79081 gaccgatacc gcccatagca accagtttct ctgctttttc taccgcgtct tcgaaatctt
    79141 tagcgcggta cattgccaga gtcggggaca gtttttcatg tgcgaacggt tcgctttcat
    79201 caacaacggt cacttcaccg atcagaatct tggtgttttc tggtacagag aagcctgcca
    79261 gttcagcaat tttataggct ggctgaccaa cgatagccgc gttcagcgca ccgtttttca
    79321 ggataacatc ctgaacagct ttcagctctt taccctgcaa cagatagccg ccgtgggttg
    79381 caaaacgttc acgtacagcg tcataaacag agtcaacaac aacaacagac tgttcagaag
    79441 cacagattac gccgttgtcg aaggttttgg acatcagtac agatgcaact gcacgtttga
    79501 tatcagcagt ttcatcgata acaactggag tgttgcccgc gcctacaccg atagctggtt
    79561 taccggagct gtatgcggct ttaaccatgc ccggaccacc agtcgcgagg atcaggttga
    79621 tgtctgggtg gtgcatcagt gcgttagaca gttcaacaga aggttgatcg atccagccga
    79681 tcagatcttt cggagcaccg gcagcgatag cagcctgcag aacgatatca gccgctttgt
    79741 tggtggcatc ttttgcacgc gggtgcgggg agaagataat ggcgttacgg gtcttcagac
    79801 tgatcagcga tttgaagata gcagttgaag tcgggttagt ggtcggaacg ataccgcaaa
    79861 taataccgat tggttcagcg atagtgatgg taccaaaagt gtcgtcttca gacagaacac
    79921 cacaggtttt ttcatcttta taggcgttgt agatatattc agaagcaaag tggtttttga
    79981 tcactttatc ttcgacgata cccatgccgg attcggcaac ggccattttc gcgagtggga
    80041 ttcgagcatc tgcagcagcc agagcggcgg cgcggaagat tttgtctact tgctcttgag
    80101 tgaaactggc atattcacgc tgggcttttt ttacacgctc tacgagtgcg ttaagttcag
    80161 cgacattagt aacagccata atgctctcct gataatgtta aactttttta gtaaatcatc
    80221 tgctcgaata cgagagtata gtcagtgcgg tgatgatttg cttaacctat gaaaatcaaa
    80281 agcttactcg cgctcacact cactgtgatt tactaaaaga gtttaaacat tagagttatt
    80341 atctctaatg cgtcacttcc aggtggcgta agcaagatta ctcacttctg ggtactgatt
    80401 acgtgatcca aatcaaattt ttgcaaagct gacacctttc agcatcgctt ttcgccatta
    80461 tagctaacag ttaataaatt gtagtatgat ttggtggcta cattagcatg ttttgcacaa
    80521 ctagataaca ataacgaatg atagcaattt taagtagtta ggaggtgaaa aatgctgtca
    80581 aaaggcgtat tgtcagcgcg tcttttcaac cttatttatg gctaacatta tccggctttt
    80641 gcttcggagc taaccgtgat tcagaccttt tttgattttc ccgtttactt caaatttttc
    80701 atcgggttat ttgcgctggt caacccggta gggattattc ccgtctttat cagcatgacc
    80761 agttatcaga cagcggcagc gcgaaacaaa actaacctta cagccaacct gtctgtggcc
    80821 attatcttgt ggatctcgct ttttctcggc gacacgattc tgcaactttt tggtatatca
    80881 atcgattcgt tccgtatcgc cgggggcatc ctggtggtga cgatagcgat gtcgatgatc
    80941 agcggcaagc ttggcgagga taaacagaac aagcaagaaa aatcagaaac cgcggtacgt
    81001 gaaagcattg gtgtggtgcc attggcgttg ccgttgatgg cggggccagg ggcgatcagt
    81061 tctaccatcg tctggggtac gcgttatcac agcattagct atctgtttgg tttctttgtg
    81121 gctattgcat tgttcgcttt atgttgctgg ggattgttcc gcatggcacc gtggctggta
    81181 cgggttttac gccagaccgg catcaacgtg attacgcgta ttatggggct attgctgatg
    81241 gcattgggga ttgaatttat cgttactggt attaagggga ttttccccgg cctgcttaat
    81301 taattccttt caaatgaaac ggagctgcca tgctccgttt acttcgtcat tatttttact
    81361 ttgttcccgc gcagttatca aaagcaaaag gaataggtaa aaatattctt ctcaaattac
    81421 agttagttat aaggatttcc ttaactgctt ctcctcacta tcatgttatt ttcgccacat
    81481 cataatcctg ggcttgctga agaataattg aaatgatatt agtaattcca ctgcctttgg
    81541 tagaggaaag tgctaaataa taatcaattg ttaaattatt gtgcatttca ctactggaac
    81601 tgtaatcaga aaagatagac atgcttagcc aatctctatt tgattgaatt gaaagatgtt
    81661 tgttaaggca tggatgcaag ctatagattc tgatacggtc aataaaagag aattgcttaa
    81721 caattttgca aaatgtattg gcgagtaaga accgcatttg gtactttccg ggcaaccgcc
    81781 agacgattct ttattggtaa tgagaataat taacaattaa agagcgtcgc gaaagaataa
    81841 tgtgtctcga caggggagac acagtacgaa tcgacataag gtgatcgtct gaatcaccag
    81901 aataaataaa gtcggtgata gtaatacgta acgataaagt aacctgacag cagaaagtct
    81961 ccgagcctgt gcagggtccc aatccgggat tacacatgct ggttaatacc agtaattata
    82021 atgagggagt ccaaaaaaca atgaccaaca tcaccaagag aagtttagta gcagctggcg
    82081 ttctggctgc gctaatggca gggaatgtcg cgctggcagc tgatgtaccc gcaggcgtca
    82141 cactggcgga aaaacaaaca ctggtacgta acaatggttc agaagttcag tcattagatc
    82201 cgcacaaaat tgaaggtgtt ccggagtcta atatcagccg agacctgttt gaaggcttac
    82261 tggtcagcga tcttgacggt catccagcac ctggcgtcgc tgaatcctgg gataataaag
    82321 acgcgaaagt ctggaccttc catttgcgta aagatgcgaa atggtctgat ggcacgccag
    82381 tcacagcaca agactttgtg tatagctggc aacgttctgt tgatccgaac actgcttctc
    82441 cgtatgccag ttatctgcaa tatgggcata tcgccggtat tgatgaaatt cttgaaggga
    82501 aaaaaccgat taccgatctc ggcgtgaaag ctattgatga tcacacatta gaagtcacct
    82561 taagtgaacc cgttccgtac ttctataaat tacttgttca cccatcaact tcaccggtgc
    82621 caaaagccgc tatcgagaaa ttcggcgaaa aatggaccca gcctggtaat atcgtcacca
    82681 acggtgccta taccttaaaa gattgggtcg taaacgaacg aatcgttctt gaacgcagcc
    82741 cgacctactg gaacaacgcg aaaaccgtta ttaaccaggt aacctatttg cctattgctt
    82801 ctgaagttac cgatgtcaac cgctaccgta gtggtgaaat cgacatgact tataacaaca
    82861 tgccgatcga attgttccag aagctgaaaa aagagatccc ggacgaagtt cacgttgatc
    82921 catacctgtg cacttactat tacgaaatta acaaccagaa accgccattc aacgatgtgc
    82981 gtgtgcgtac cgcactgaaa ctaggtatgg accgcgatat cattgttaat aaagtgaaag
    83041 cgcagggcga catgcccgcc tatggttaca ctccaccgta tactgatggc gcaaaattga
    83101 ctcagccgga atggtttggc tggagccagg aaaaacgtaa cgaagaagcg aaaaaactgc
    83161 tggctgaagc gggttatacc gcagacaaac cgttgaccat caacctgttg tataacacct
    83221 ccgatctgca taaaaagctg gcgattgctg cctcttcatt gtggaagaaa aacattggtg
    83281 taaacgtcaa actggttaac caggagtgga aaacgttcct cgacacccgt caccagggta
    83341 cttttgatgt ggcccgtgca ggctggtgtg ctgactacaa cgaaccaact tccttcctga
    83401 acaccatgct ttcgaacagc tcgatgaata ccgcgcatta taagagcccg gcctttgaca
    83461 gcattatggc ggaaacgctg aaagtgactg acgaggcgca gcgcacagct ctgtacacta
    83521 aagcagaaca acagctggat aaggattcgg ccattgttcc tgtttattac tacgtgaatg
    83581 cgcgtctggt gaaaccgtgg gttggtggct ataccggcaa agatccgctg gataatacct
    83641 atacccggaa tatgtacatt gtgaagcact aatggcaata cgtggggcag gagtgtcctg
    83701 ctccacggtg tctgattttt atcgcattac agaaggcaca ggccagaagg tagggcaatg
    83761 ttaaaattta ttctacgtcg ctgtctggaa gcgattccga cgctatttat tcttattact
    83821 atttcgttct ttatgatgcg cctcgcgccg ggaagccctt ttaccggcga acgtacttta
    83881 ccgccagaag tgatggccaa tatcgaagcg aaatatcatc ttaatgatcc gatcatgaca
    83941 cagtatttca gctacctgaa acaactggcg cacggtgatt tcggtccatc gtttaaatat
    84001 aaagattatt cggtcaatga cttggttgca tccagttttc ccgtttctgc caaactggga
    84061 gccgcagcat ttttccttgc ggtaatactg ggtgttagtg ctggcgttat tgccgcatta
    84121 aaacaaaaca ccaaatggga ctataccgtg atggggctgg caatgaccgg ggttgttatc
    84181 cccagttttg tggttgcacc attattagtc atgatatttg cgatcatttt gcattggctg
    84241 ccgggcggtg gctggaatgg tggggcgctt aaattcatga tattgccgat ggtggcgttg
    84301 tcactcgctt atatcgccag tattgcgcgt attacccgtg gctctatgat tgaagtatta
    84361 cactccaatt ttattcgtac tgcccgggcg aaagggttac ctatgcggcg gatcatttta
    84421 cgtcacgcat taaaacctgc tctgttaccc gtgctctctt atatgggccc cgcatttgtc
    84481 ggcattatta ccggttctat ggttatcgaa accatttatg gtttgccggg gattggacaa
    84541 ctgttcgtta atggcgcatt gaaccgtgac tattccttag tgttaagcct gaccatcctg
    84601 gttggtgctt taaccatttt gtttaatgcc attgtcgatg tgctatatgc ggttatcgac
    84661 ccgaaaatcc gttactgata ctggagctcg cgatgatgtt aagtaagaaa aacagcgaga
    84721 cgctggaaaa tttcagtgaa aagctggagg tcgaagggcg aagcttgtgg caggacgcac
    84781 gtcgacgttt tattcataac cgtgcggcgg ttgccagtct gatagtgctg gtgctgatcg
    84841 cgttatttgt aatcctggca ccgatgcttt cccagtttgc ctatgacgat actgactggg
    84901 cgatgatgtc cagcgccccg gatatggagt ccggtcacta ctttggtact gactcatccg
    84961 gtcgcgacct gcttgtgcgc gttgcgattg gtgggcgtat ctcactcatg gtcggtgttg
    85021 ctgcggcact ggtggctgtg gtcgtcggga cactttatgg ttcgctttcc ggttatctgg
    85081 gcggtaaagt ggattcggta atgatgcgtc tgctggaaat cctcaactcc ttcccattca
    85141 tgttcttcgt cattttgctg gtgacctttt tcggtcaaaa catcctgctg attttcgtgg
    85201 cgataggcat ggtttcctgg ctggatatgg ctcgtattgt gcgtgggcaa accctgagtc
    85261 tgaagcgcaa agagtttatt gaggcggcac aagttggcgg tgtatcgaca tcgggcattg
    85321 ttattcgcca cattgtgccg aatgtactcg gtgtggtggt ggtctacgca tcacttctgg
    85381 tgcccagcat gatcctcttt gaatctttcc ttagcttcct ggggttgggt acgcaagagc
    85441 cgttaagcag ctggggggca ttgctgagtg atggcgcgaa ctcgatggaa gtctctccat
    85501 ggttattgtt gttcccagcg ggattcctcg tggtgacgct gttttgtttc aactttatcg
    85561 gcgatggctt gcgtgatgcc ctcgacccga aagatcgtta aggagtgcag ccatgagcgt
    85621 aattgaaact gcaactgtgc cgctcgcaca acaacaggct gacgcactgc tgaacgtgaa
    85681 agatttgcgt gtcaccttta gtaccccgga cggcgacgtc acggcggtca atgatttgaa
    85741 tttttcccta cgtgccggag aaacgctggg tattgtaggt gagtctggtt cgggtaaatc
    85801 gcaaactgca tttgcgttga tgggcctgtt ggctgccaac ggacgtattg gcggatcggc
    85861 aaccttcaat gggcgtgaaa tcctcaattt gccagagcat gaactcaata aactgcgcgc
    85921 tgaacaaatc tcaatgattt ttcaggaccc aatgacttcg ttgaatccct atatgcgcgt
    85981 cggtgagcag ttaatggaag tgctgatgct gcataagaac atgagcaaag ctgaggcgtt
    86041 tgaagagtcg gtgcggatgc tcgatgcggt aaaaatgccg gaagcgcgta aacgcatgaa
    86101 aatgtacccg cacgaatttt ctggcggcat gcgtcagcga gtcatgattg cgatggcatt
    86161 gctatgtcga cctaagctac tgattgcgga tgaacccact actgcgctgg acgtcaccgt
    86221 acaggcgcag atcatgacgc tattaaatga actgaagcgg gaatttaata ccgccatcat
    86281 tatgatcacc cacgatcttg gcgtggtggc ggggatctgc gacaaagtgc tggtaatgta
    86341 cgccgggcgc acgatggaat atggcaatgc gcgcgatgtc ttttatcaac ccgttcatcc
    86401 ttattctatc ggtttgctca acgcggtgcc gcgtcttgat gcggaaggtg aaacaatgtt
    86461 gaccatccct ggtaatccgc caaacttgct gcgattaccg aaaggttgcc cgttccagcc
    86521 acgttgtcca catgcgatgg aaatttgtag tagcgcaccg ccgctggaag agtttacgcc
    86581 tggccgtctg cgtgcttgct ttaaaccggt ggaggaactg ttatgaatgc tgtaactgaa
    86641 ggaagaaaag tcctccttga aatcgccgat cttaaagtgc actttgaaat caaagatggc
    86701 aaacagtggt tctggcaacc gccgaaaacg ctcaaagccg tcgatggtgt cactcttcgc
    86761 ctgtatgaag gggaaacatt aggtgtggta ggggaatcgg gatgcggtaa gtccaccttt
    86821 gctcgcgcca tcatcggttt ggtcaaggcg accgacggtc atgttgcctg gttaggtaaa
    86881 gagttgctgg gcatgaagcc cgatgaatgg cgtgccgttc gcagtgatat tcagatgatt
    86941 ttccaggatc cgttggcatc gctaaacccg cgtatgacca tcggcgagat catcgctgaa
    87001 ccactgcgta cttatcatcc gaaaatgtca cgccaggaag ttcgcgagcg cgtgaaggcg
    87061 atgatgctga aagtcgggtt attgcctaac ctgattaacc gctatccgca tgagttctcc
    87121 ggtgggcagt gccagcgtat cgggattgct cgtgctctta ttcttgaacc gaagctgatt
    87181 atctgcgatg agccggtttc ggcgctggac gtgtcaattc aggcgcaggt ggtcaacctg
    87241 ctccagcagc tgcaacgtga gatgggattg tcattaattt ttatcgctca tgacctggcc
    87301 gtggtaaaac acatttccga tcgtgtgttg gtgatgtatc tcggccatgc ggtagaactg
    87361 gggacctatg atgaggtcta ccacaatcca ctacatcctt acaccagggc attgatgtcg
    87421 gcagtcccca tacctgatcc ggatctggag aagaacaaaa ccatccagtt actggaaggg
    87481 gaattaccgt cgccgatcaa cccgccttcc ggttgtgttt tccgtacccg ttgcccgatt
    87541 gccggtccgg agtgcgccaa aacacgtcct gttctggagg ggagtttcag acacgccgtt
    87601 tcttgcctga aagtcgatcc gctttaaaca caataagggc tgacagttgt cagccctttt
    87661 tcacgccgaa agcgattatt tattcccgcc agatgatatg gcaaagtttg tgatcttttt
    87721 cgcggcataa cagaatgcgg gcgaaaacat cgttgatttc accatcttca ctgtccgcca
    87781 gaccaatcac cacttcggca aaaaagtctg ggttcaaatc gaaatcaaca tgttcctgcc
    87841 agtcttccgc cggatcgaat aactccgcgc cgccgcgctc ttcaaactga agattgaaca
    87901 gcagaacatc agctggatcg agattgtcgg cagccagttc gagaaaaata tcgtaggcct
    87961 gctcaagcgt ttcatcttca gtcaggcgat tgtttagatc catatccata gtcactacct
    88021 gtttaacctc tgttggcgac gttttacagc aacggactga agaagtaaaa cagtcgctcg
    88081 gcgacacgtt gccataatgg acgttttagc cataaacggg catcgagcag acgtgaacgc
    88141 gaaatataat cgtcctgaac ggcggcaagg tctgcaccaa aacctttatc gtcgattgcc
    88201 agggtaatct cgaaattcag ccacagactc cgcatatcaa ggttaacggt gccaaccaga
    88261 cttagttcgc catcgaccag cacgctcttg gtatgcagta acccgccttc aaactgataa
    88321 attttaaccc cagcagcaag cagttccgta aagaatgcgc gactggccca gccgaccagc
    88381 atcgagtcat tttttcgcgg aaggataata ctgacatcca ccccgcgctg cgccgccgtg
    88441 caaatcgcat gaagtaaatc atcgcttggc acaaagtagg gcgtggtcat gatcaaatat
    88501 tcacgcgccg aataagccgc agtcaataat gcctggtgaa tgagatcttc cggaaagccg
    88561 gggccagaag caattgtgtg aatggtgtga ccgctggcct gttcaaacgg cataatattg
    88621 acatctggtg gtggcggcag aatacgtttt ccggtttcaa tctcccagtc gcaggaataa
    88681 ataatcccca tcgcggtggc gatggggcct tccatacgcg ccatcagatc aatccattgc
    88741 cctacgcccg catcttgttt gaagtagcga ggatcgacca tattcatgct gccggtgtac
    88801 gcgatgtaat tatcgatcat gatcatcttg cgatgttggc gcaggtccat acggcgtaaa
    88861 aacacacgca tcagattgac ctttaaggct tcgaccactt caataccggc attacgcatt
    88921 agcttgggcc acgggctgcg gaaaaaagcc acactcccgg cggagtcgag catcaatcgg
    88981 caatgaatac cacgccgtgc agccgccatt aatgattcag ccacctggtc cgccatgccg
    89041 ccgggctgcc agatataaaa caccatctca atattatggc gcgcgagctg gatgtcgcgg
    89101 attaacgcct gcatcacatc atctgactcg gtcatcagtt gtagctgatt ccctttgacc
    89161 ccagcgatcc cctgacgacg ctcgcaaagc ttgaataatg gcgcagcgac actgctattt
    89221 tcttcggcga agatatgctt acaggcttta aggtcgttaa gccattttgc ggtggaaggc
    89281 cacatcgctc tggcgcgctc agcgcggcgt ttgcctaaat ggagctcgcc aacggcaaga
    89341 taggcaataa ttccgactaa cggcagaatg taaataatca acagccaggc catcgcggag
    89401 ggaactgcgc gtcgtttcat tagaatgcgt aaagttacgc ctgcaatgag caaccagtat
    89461 cccagaatgg ccaaccaact caccaacgta taaacggttg tcatagatta gaaatccttt
    89521 tgaaagcgca ttgttatgag tttacgcata tggattcatc tggcaaataa aaacgcggga
    89581 aaagcactgg tcagcgtcgg gggtgggggt ataatgacca ttctgttatt gcatagagta
    89641 gttaacatga agcggagtag aacggaagtg gggcgctggc gcatgcagcg tcaggctagc
    89701 cgacgtaaat cgcgttggct tgaggggcaa tcgcgccgaa atatgcgtat ccacagcatc
    89761 aggaagtgca ttctaaacaa acagcgtaac tcgttattgt ttgcgatcta caatatctaa
    89821 atatactggg caccattgcg gtgcccaata agtttactct ttcgtcttta aactctattt
    89881 ttgcactgat tctttactgt cagctttcgt ttcttgttca tcgttatcgc tgaaaatgcc
    89941 atgaccggag ttcaacaaca ttgaaacgag catatcatta ttaatctctt caccatttaa
    90001 aactcgcgcc agaatttcgc tgccaaacag ttgcggtgag agaatgatat ccggatgtac
    90061 catcttaatc ttattcaggt ttttgctatc actgacggcg agaactgttt tgacatcact
    90121 gctcatatct tttgccgaga gtacaacaaa cgcgttatct gcatcgttat cactcagcgc
    90181 cagaatggcc cggcaacgat cgattcccgc tttctttaat actgaactgt cattactgtc
    90241 accggggata acatcagcgt tatcgcctaa gcgttgctca agttgcttga tatcatcttc
    90301 aggcaagttg ctgataaccg ttacgttttg tccgcgttga ttcagttgca ggatcgtatt
    90361 gatggcgaga atcgaatgtc cgcaaacaat aaaatgatct ttacgatgca ttgtatgatt
    90421 gtttcctttt acaagtttgt tgaatccccc gcggataagc ggaccaaaaa ttgaggtcat
    90481 agatgtggca aaaacggtaa tgccggaaat aatgaccgaa atagtgaaca atcgtgctga
    90541 ttcagaaaca gggacaatat cgccatagcc gacggttgac atggtttcta tcgaaaaata
    90601 gaacgcggtc atcaaacttt ctattcgcgg attaaaacct tcgcttaaat aaagcgcacc
    90661 gtaggttgag taaaacagta atgtcgtgaa actaataaat gcaaaaattg tcccggctgc
    90721 ggcgctactg tgggagaagt ctttgcgcag tatcagcaaa aacaccagcg taaaaatgca
    90781 aaatccaata ctaaatttca gccagggata aaaatgcagg gtgtaaatta gcgctatcaa
    90841 caacaaaatg atactgattg cccatgcgag ctttgcccgg aacagtaagc caatggagtt
    90901 caggacaaga aagacgccga gcataaatag tggggcgttt gccagcaagg agagatccaa
    90961 ctctgaaaat gctttgatat ggaaaatatc aagcaaattg accgacatac catagattgt
    91021 tataaaaata agcaatccat ttaaaaagac ggccagcgcg agaatgtcgt gccgaagggt
    91081 cacccataaa tttgttgctg tttgtttgaa tgtagcccag tgactcactg atacctctgt
    91141 aaccacaatt ttgtttaatt atcttagcgt gcacattttc tgaacataca tgcagcgcgt
    91201 gaatgtgtta aacaatagta gatgacttta tccgcagtaa tcaaaggatt tgagcgagca
    91261 actgtacctc aggaaaggtc atttttcctg aatatgctca catcatataa agaaatacag
    91321 ataaagttat tatctgcttg tggtagtgaa tgcactgacc ggctataagg aaaggccaaa
    91381 caagaacacg gttgcaaaaa ccgtgccctt aaatattgaa tctctattca gaacactttc
    91441 ttaaatggtt tcactgaaac gtgttcatag acacctgccg ctacgtacgg gtcagcatcg
    91501 gcccaggcct gagctgcttc cagcgattca aattcagcaa taacggttga gccagtaaat
    91561 cccgcagccc ctggatcgtt actgtctacc gctggcattg gaccagctgt caacaaacga
    91621 ccttcatcat gcagtaactg taaacgtgct aaatgtgccg gacgaacgga aaggcgtttt
    91681 tcgaggctat cagctttgtc ttgagcgtaa ataacataca acacgggcac aactccttgt
    91741 tcgggaaagt tgtaaagtac gttatttgaa agggcgaaga tctgcaacgg aaagatgatg
    91801 tctttgttaa gaccatgcat aaagtaaggt caattacgcc aaaaatgaca ttttcactga
    91861 tccagatcgt catgccttat tgaatatgat tgctatttgc atttaaaatc gagacctggt
    91921 ttttctactg aaatgattat gacttcaatg acccttgatt tacctcgccg cttcccctgg
    91981 ccgacgttac tttcggtctg cattcatggt gctgttgtgg cgggtctgct ctatacctcg
    92041 gtacatcagg ttattgaact acctgcgcct gcgcagccaa tttctgtcac gatggttgcg
    92101 cctgctgatc tcgaaccgcc acaagccgtt cagccgccac cggagccggt ggtagagcca
    92161 gaaccggaac ctgagccgat ccccgaaccg ccaaaagaag caccggtggt cattgaaaag
    92221 ccgaagccga aacctaagcc aaaaccgaag ccggtgaaaa aggtacagga gcagccaaaa
    92281 cgcgatgtca aacccgtaga gtcgcgtccg gcatcaccgt ttgaaaatac ggcacccgca
    92341 cgcctgacat caagtacggc aacggctgca accagcaagc cggttaccag tgtggcttca
    92401 ggaccacgcg cattaagccg taatcagccg cagtatccgg cacgagcaca ggcattgcgc
    92461 attgaagggc aggttaaagt taaatttgac gtcacgccgg atggtcgcgt ggataacgta
    92521 caaatcctct cagccaagcc tgcgaacatg tttgagcgtg aggtgaaaaa tgcgatgcgc
    92581 agatggcgtt atgagccggg taagccaggc agtgggattg tggtgaatat cctgtttaaa
    92641 attaacggca ccaccgaaat tcagtaagca gaaagtcaaa agcctccgac cggaggcttt
    92701 tgactattac tcaacaggta aggcgcgagg ttttccttca ggatcaaccg cgacatactt
    92761 aaataatgct tctgtcgctt tatagcgttg cccgattggt tcagacgcta cttttttcac
    92821 ccacacttca atattaatgc tgaccgatgt cgtccctttc tggacacagc gtgcatagca
    92881 gcacaccaca tcgccgaccg caaccggccg taagaaagtc attccttcaa cccgcacagt
    92941 cactacgcga ccgtgggcaa tttctttcgc cagaatagcg ccgccaatat ccatttgtga
    93001 cattaaccaa ccaccaaaga tgtcaccatt ggcattggta tcggcgggca tggctaaagt
    93061 acgtaaaaca agatcgccct gagggacgtt atgtgttgta gacatggtaa aaccgactta
    93121 aaaggaaaat cacaggcgcg atgctactat gattttcctg aggagaacag aggagataca
    93181 gcgccagccc cgaaggactg gcagtctggt taggatttat cttcctgcgg catgtggcgg
    93241 tagatataga taccgcttaa caatgtgaag attaaggtaa gggcggtcag gccaaagact
    93301 ttaaagttga cccaaatatt ttgcggcagc cagaatgcga tgtagatgtt tgccagaccg
    93361 caaaggataa agaaaacagc ccaggccaga tttagcttcg accataccgg ttgcggcagc
    93421 gtgagttctt tacccagcat ccgctgaatt agcggctttt tcatcaccca ttggctgact
    93481 aacagggctc ccgcaaacag agcataaatg actgtaacct tccatttaat aaactcatca
    93541 ttgtggaaga acagcgtcaa gccaccgaag acgaccacca gaacaaaagt gatcagggcc
    93601 atcttctcaa ccttacgaaa gcgaacccag ctatagataa gcacaatcgc cgtggcgacg
    93661 atcagcgccg cagtagccgc atagatgtca taaatcttgt aaaacgcgaa aaagacaacc
    93721 agcggtaaaa aatcaagaaa ctgcttcatt ttacgattcc gtaatcaaat gcttaaggtt
    93781 attggcgaat caacatatac aggcggaaca gatagatgag caatatggca gaaatcaggt
    93841 tgctcaaggt gttcgccagt acggcaccaa tttccggggt taatgcggca aaagaagagg
    93901 caaaaagcag caacagtgtt tttgccagca accagctcag tactgcgggt gccaccagac
    93961 gcatattcgc ccaagtcagc cgcatactgc tacgcatcga ggcaaaaatg cccattttgt
    94021 cctgtaccag catcaccggt gccagggcca gtaaaatggc catgataatt cccggaacga
    94081 ccaccagcat gatgccaatc tgtactaaaa gggtagtcag aaaaatcaga ataaataact
    94141 tgggcaatat cggcgcactg gcaccaatag ctcgcagcgc actgactctc tgacccgcag
    94201 acaccagctg gataattaat attacgcctc cggcgagaat ggcgttaccg attaatcctg
    94261 aaaaagtgga cgccgctgaa gcctgcagca aaatttgttg ctgttccggt gacatattct
    94321 gaaccaggtc gaacaaccca ctactgccgc taacgggcac gccgtcattg agctgcgcaa
    94381 gctgtgcatc actgggtgag aaaacatgcc ctaacaccac tgtgataaac gcacatagca
    94441 acgataccaa cagaatggtc ataaattgat tacggaagaa atttccggtg tcacggtata
    94501 cggactgcgc cgtgatagac atgcactctc cttgagtttt atgcaggtgt taattagcgg
    94561 gcaattgtac cctggttaag cctcgggtgg cagcatcagg tcttgtatgg aaaagcatat
    94621 ctttgtaaag cggaggtaat tgctggcctt caggcgtggg caaaccgcgg caagtggcga
    94681 acgagttaat atttgcgtag cgaaaatatt taaaaattga tttaaatcac gttaaccagg
    94741 attctcaatg caacttctaa attaatccag atcaataaag ggtgaattat catatgtaat
    94801 gtgatctatg taggatcatt tgttactcca atgtaggtat attcgtcacg tttttataac
    94861 cataacgacg gagcggatat gaaaaagtta acagtggcgg ctttggcagt aacaactctt
    94921 ctctctggca gtgcctttgc gcatgaagca ggcgaatttt ttatgcgtgc aggttctgca
    94981 accgtacgtc caacagaagg tgctggtggt acgttaggaa gtctgggtgg attcagcgtg
    95041 accaataaca cgcaactggg cctgacgttt acttatatgg cgaccgacaa cattggtgtg
    95101 gaattactgg cagcgacgcc gttccgccat aaaatcggca cccgggcgac cggcgatatt
    95161 gcgaccgttc atcatctgcc gccaacactg atggcgcagt ggtattttgg tgatgccagc
    95221 agcaaattcc gtccttatgt tggggcaggt attaactaca ccaccttctt tgataatgga
    95281 tttaacgatc atggcaaaga ggcggggctt tccgatctca gtctgaaaga ttcctgggga
    95341 gctgccgggc aggtgggggt tgattatctg attaaccgtg actggttggt taacatgtca
    95401 gtgtggtaca tggatatcga taccacggct aagtataaat ctggtgttac taccgtgaaa
    95461 gactcggtac gactggatcc gtgggtgttt atgttctccg caggatatcg tttttaattt
    95521 tctctgcaaa acctctgcaa aacccctctg caaaactggt catcaaatga ccagtttttt
    95581 ccattcctta ccgcgtgcgt cgttgtaaat atcg
//
LOCUS       DAZKHC020000005        88772 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZKHC020000005 DAZKHC020000000
VERSION     DAZKHC020000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 88772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 88772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.005"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..640
                     /gene="casA"
                     /locus_tag="ACHOSM_RS02070"
                     /old_locus_tag="pgaptmp_000414"
                     /gene_synonym="cse1"
                     /pseudo
     CDS             1..640
                     /gene="casA"
                     /locus_tag="ACHOSM_RS02070"
                     /old_locus_tag="pgaptmp_000414"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            637..1170
                     /gene="casB"
                     /locus_tag="ACHOSM_RS02075"
                     /old_locus_tag="pgaptmp_000415"
                     /gene_synonym="cse2"
     CDS             637..1170
                     /gene="casB"
                     /locus_tag="ACHOSM_RS02075"
                     /old_locus_tag="pgaptmp_000415"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HGU4557006.1"
                     /translation="MSIVKDEHKVALRKWHEELQDKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMKKGRFTRLL
                     AVKTPDDLLRQLRRAVKLLNGSVNLDSLAEGGFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQN"
     gene            1185..2258
                     /gene="cas7e"
                     /locus_tag="ACHOSM_RS02080"
                     /old_locus_tag="pgaptmp_000416"
     CDS             1185..2258
                     /gene="cas7e"
                     /locus_tag="ACHOSM_RS02080"
                     /old_locus_tag="pgaptmp_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HGU4557007.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDSGSPKTVILGGATRLRVSSQSL
                     KRAWRTSELFEQALAGHIGVRSGRIAREAATILIEKGIEDKKAIEWAVEIADYLGKAK
                     KDKKQKNDKKPKDPLTSAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEKDLALLRK
                     DRMAVDIAMFGRMLAKKPGFNVEAACQVAHAFGVSETIVENDFFTAVDDLRQASEDAG
                     AGHVDETGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNS
                     FASRAYASWALAEKGTDQPRSLAAAFYEPINGTRQLEVAVQRITTLRENMNTVYEQKT
                     DYASFDVMNKQGSMKDVLDFICA"
     gene            2269..3015
                     /gene="cas5e"
                     /locus_tag="ACHOSM_RS02085"
                     /old_locus_tag="pgaptmp_000417"
     CDS             2269..3015
                     /gene="cas5e"
                     /locus_tag="ACHOSM_RS02085"
                     /old_locus_tag="pgaptmp_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HGU4557008.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDTLRNAYQQYQDHFRKLKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            2997..3647
                     /gene="cas6e"
                     /locus_tag="ACHOSM_RS02090"
                     /old_locus_tag="pgaptmp_000418"
     CDS             2997..3647
                     /gene="cas6e"
                     /locus_tag="ACHOSM_RS02090"
                     /old_locus_tag="pgaptmp_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HGU4557009.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRPFVSELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVKGQAEGRDIWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCALMLIKPGAEA"
     gene            3644..4567
                     /gene="cas1e"
                     /locus_tag="ACHOSM_RS02095"
                     /old_locus_tag="pgaptmp_000419"
     CDS             3644..4567
                     /gene="cas1e"
                     /locus_tag="ACHOSM_RS02095"
                     /old_locus_tag="pgaptmp_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HGU4557010.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRGTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHWGRGG"
     gene            4564..4857
                     /gene="cas2e"
                     /locus_tag="ACHOSM_RS02100"
                     /old_locus_tag="pgaptmp_000420"
     CDS             4564..4857
                     /gene="cas2e"
                     /locus_tag="ACHOSM_RS02100"
                     /old_locus_tag="pgaptmp_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HGU4557011.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     repeat_region   4954..5227
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4954..4982
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaaccg"
     gene            complement(5248..6285)
                     /gene="iap"
                     /locus_tag="ACHOSM_RS02105"
                     /old_locus_tag="pgaptmp_000421"
     CDS             complement(5248..6285)
                     /gene="iap"
                     /locus_tag="ACHOSM_RS02105"
                     /old_locus_tag="pgaptmp_000421"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HGU4557012.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            6537..7445
                     /gene="cysD"
                     /locus_tag="ACHOSM_RS02110"
                     /old_locus_tag="pgaptmp_000422"
     CDS             6537..7445
                     /gene="cysD"
                     /locus_tag="ACHOSM_RS02110"
                     /old_locus_tag="pgaptmp_000422"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HGU4557013.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEVVAMGINP
                     FIHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            7447..8874
                     /gene="cysN"
                     /locus_tag="ACHOSM_RS02115"
                     /old_locus_tag="pgaptmp_000423"
     CDS             7447..8874
                     /gene="cysN"
                     /locus_tag="ACHOSM_RS02115"
                     /old_locus_tag="pgaptmp_000423"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HGU4557014.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            8874..9479
                     /gene="cysC"
                     /locus_tag="ACHOSM_RS02120"
                     /old_locus_tag="pgaptmp_000424"
     CDS             8874..9479
                     /gene="cysC"
                     /locus_tag="ACHOSM_RS02120"
                     /old_locus_tag="pgaptmp_000424"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HGU4557015.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            9529..9852
                     /gene="ygbE"
                     /locus_tag="ACHOSM_RS02125"
                     /old_locus_tag="pgaptmp_000425"
     CDS             9529..9852
                     /gene="ygbE"
                     /locus_tag="ACHOSM_RS02125"
                     /old_locus_tag="pgaptmp_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HGU4557016.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            10046..10357
                     /gene="ftsB"
                     /locus_tag="ACHOSM_RS02130"
                     /old_locus_tag="pgaptmp_000426"
     CDS             10046..10357
                     /gene="ftsB"
                     /locus_tag="ACHOSM_RS02130"
                     /old_locus_tag="pgaptmp_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HGU4557017.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            10376..11086
                     /gene="ispD"
                     /locus_tag="ACHOSM_RS02135"
                     /old_locus_tag="pgaptmp_000427"
     CDS             10376..11086
                     /gene="ispD"
                     /locus_tag="ACHOSM_RS02135"
                     /old_locus_tag="pgaptmp_000427"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGU4557018.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAVPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            11086..11565
                     /gene="ispF"
                     /locus_tag="ACHOSM_RS02140"
                     /old_locus_tag="pgaptmp_000428"
     CDS             11086..11565
                     /gene="ispF"
                     /locus_tag="ACHOSM_RS02140"
                     /old_locus_tag="pgaptmp_000428"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HGU4557019.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            11562..12611
                     /gene="truD"
                     /locus_tag="ACHOSM_RS02145"
                     /old_locus_tag="pgaptmp_000429"
     CDS             11562..12611
                     /gene="truD"
                     /locus_tag="ACHOSM_RS02145"
                     /old_locus_tag="pgaptmp_000429"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HGU4557020.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            12592..13353
                     /gene="surE"
                     /locus_tag="ACHOSM_RS02150"
                     /old_locus_tag="pgaptmp_000430"
     CDS             12592..13353
                     /gene="surE"
                     /locus_tag="ACHOSM_RS02150"
                     /old_locus_tag="pgaptmp_000430"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HGU4557021.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            13347..13973
                     /gene="pcm"
                     /locus_tag="ACHOSM_RS02155"
                     /old_locus_tag="pgaptmp_000431"
     CDS             13347..13973
                     /gene="pcm"
                     /locus_tag="ACHOSM_RS02155"
                     /old_locus_tag="pgaptmp_000431"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HGU4557022.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            14158..15252
                     /gene="nlpD"
                     /locus_tag="ACHOSM_RS02160"
                     /old_locus_tag="pgaptmp_000432"
     CDS             14158..15252
                     /gene="nlpD"
                     /locus_tag="ACHOSM_RS02160"
                     /old_locus_tag="pgaptmp_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HGU4557023.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQSQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            15315..16307
                     /gene="rpoS"
                     /locus_tag="ACHOSM_RS02165"
                     /old_locus_tag="pgaptmp_000433"
     CDS             15315..16307
                     /gene="rpoS"
                     /locus_tag="ACHOSM_RS02165"
                     /old_locus_tag="pgaptmp_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HGU4557024.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(16428..16835)
                     /locus_tag="ACHOSM_RS02170"
                     /old_locus_tag="pgaptmp_000434"
     CDS             complement(16428..16835)
                     /locus_tag="ACHOSM_RS02170"
                     /old_locus_tag="pgaptmp_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGU4557025.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            16982..17575
                     /locus_tag="ACHOSM_RS02175"
                     /old_locus_tag="pgaptmp_000435"
     CDS             16982..17575
                     /locus_tag="ACHOSM_RS02175"
                     /old_locus_tag="pgaptmp_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HGU4557026.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHTWRWQGLPQARNFSQENE"
     gene            17575..19002
                     /locus_tag="ACHOSM_RS02180"
                     /old_locus_tag="pgaptmp_000436"
     CDS             17575..19002
                     /locus_tag="ACHOSM_RS02180"
                     /old_locus_tag="pgaptmp_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HGU4557027.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRSKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTTMLAARK"
     gene            19013..19249
                     /locus_tag="ACHOSM_RS02185"
                     /old_locus_tag="pgaptmp_000437"
     CDS             19013..19249
                     /locus_tag="ACHOSM_RS02185"
                     /old_locus_tag="pgaptmp_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HGU4557028.1"
                     /translation="MICPRCADEQIEVMVKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(19289..20653)
                     /gene="ygbN"
                     /locus_tag="ACHOSM_RS02190"
                     /old_locus_tag="pgaptmp_000438"
     CDS             complement(19289..20653)
                     /gene="ygbN"
                     /locus_tag="ACHOSM_RS02190"
                     /old_locus_tag="pgaptmp_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HGU4557029.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(20742..21518)
                     /gene="ygbM"
                     /locus_tag="ACHOSM_RS02195"
                     /old_locus_tag="pgaptmp_000439"
     CDS             complement(20742..21518)
                     /gene="ygbM"
                     /locus_tag="ACHOSM_RS02195"
                     /old_locus_tag="pgaptmp_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HGU4557030.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(21523..22161)
                     /gene="ygbL"
                     /locus_tag="ACHOSM_RS02200"
                     /old_locus_tag="pgaptmp_000440"
     CDS             complement(21523..22161)
                     /gene="ygbL"
                     /locus_tag="ACHOSM_RS02200"
                     /old_locus_tag="pgaptmp_000440"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HGU4557031.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYTTGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(22158..23420)
                     /gene="otnK"
                     /locus_tag="ACHOSM_RS02205"
                     /old_locus_tag="pgaptmp_000441"
     CDS             complement(22158..23420)
                     /gene="otnK"
                     /locus_tag="ACHOSM_RS02205"
                     /old_locus_tag="pgaptmp_000441"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HGU4557032.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYVVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(23417..24325)
                     /gene="ltnD"
                     /locus_tag="ACHOSM_RS02210"
                     /old_locus_tag="pgaptmp_000442"
     CDS             complement(23417..24325)
                     /gene="ltnD"
                     /locus_tag="ACHOSM_RS02210"
                     /old_locus_tag="pgaptmp_000442"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HGU4557033.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            24521..25288
                     /gene="ygbI"
                     /locus_tag="ACHOSM_RS02215"
                     /old_locus_tag="pgaptmp_000443"
     CDS             24521..25288
                     /gene="ygbI"
                     /locus_tag="ACHOSM_RS02215"
                     /old_locus_tag="pgaptmp_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HGU4557034.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAYEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(25339..25995)
                     /gene="pphB"
                     /locus_tag="ACHOSM_RS02220"
                     /old_locus_tag="pgaptmp_000444"
     CDS             complement(25339..25995)
                     /gene="pphB"
                     /locus_tag="ACHOSM_RS02220"
                     /old_locus_tag="pgaptmp_000444"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU4557035.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGGYQLLQSRLHQLSFYPETD
                     LLISTGDNIDRGPKSLNVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEIINDNIKYAIAHADYPGDEYQFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(26101..28662)
                     /gene="mutS"
                     /locus_tag="ACHOSM_RS02225"
                     /old_locus_tag="pgaptmp_000445"
     CDS             complement(26101..28662)
                     /gene="mutS"
                     /locus_tag="ACHOSM_RS02225"
                     /old_locus_tag="pgaptmp_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGU4557036.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            28738..28917
                     /locus_tag="ACHOSM_RS02230"
                     /old_locus_tag="pgaptmp_000446"
     CDS             28738..28917
                     /locus_tag="ACHOSM_RS02230"
                     /old_locus_tag="pgaptmp_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557037.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     EPFFFRVIVPFKIKM"
     gene            28949..29302
                     /gene="ygbA"
                     /locus_tag="ACHOSM_RS02235"
                     /old_locus_tag="pgaptmp_000447"
     CDS             28949..29302
                     /gene="ygbA"
                     /locus_tag="ACHOSM_RS02235"
                     /old_locus_tag="pgaptmp_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HGU4557038.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPVKREEMKQIMRWAGPRMLWRHPILTVLHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(29339..31417)
                     /gene="flhA"
                     /locus_tag="ACHOSM_RS02240"
                     /old_locus_tag="pgaptmp_000448"
     CDS             complement(29339..31417)
                     /gene="flhA"
                     /locus_tag="ACHOSM_RS02240"
                     /old_locus_tag="pgaptmp_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HGU4557039.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVNSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(31491..32501)
                     /gene="hypE"
                     /locus_tag="ACHOSM_RS02245"
                     /old_locus_tag="pgaptmp_000449"
     CDS             complement(31491..32501)
                     /gene="hypE"
                     /locus_tag="ACHOSM_RS02245"
                     /old_locus_tag="pgaptmp_000449"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HGU4557040.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEASLPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(32498..33619)
                     /gene="hypD"
                     /locus_tag="ACHOSM_RS02250"
                     /old_locus_tag="pgaptmp_000450"
     CDS             complement(32498..33619)
                     /gene="hypD"
                     /locus_tag="ACHOSM_RS02250"
                     /old_locus_tag="pgaptmp_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HGU4557041.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(33619..33891)
                     /gene="hypC"
                     /locus_tag="ACHOSM_RS02255"
                     /old_locus_tag="pgaptmp_000451"
     CDS             complement(33619..33891)
                     /gene="hypC"
                     /locus_tag="ACHOSM_RS02255"
                     /old_locus_tag="pgaptmp_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HGU4557042.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(33882..34754)
                     /gene="hypB"
                     /locus_tag="ACHOSM_RS02260"
                     /old_locus_tag="pgaptmp_000452"
     CDS             complement(33882..34754)
                     /gene="hypB"
                     /locus_tag="ACHOSM_RS02260"
                     /old_locus_tag="pgaptmp_000452"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HGU4557043.1"
                     /translation="MCTTCGCGKGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(34758..35108)
                     /gene="hypA"
                     /locus_tag="ACHOSM_RS02265"
                     /old_locus_tag="pgaptmp_000453"
     CDS             complement(34758..35108)
                     /gene="hypA"
                     /locus_tag="ACHOSM_RS02265"
                     /old_locus_tag="pgaptmp_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU4557044.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            35320..35781
                     /gene="hycA"
                     /locus_tag="ACHOSM_RS02270"
                     /old_locus_tag="pgaptmp_000454"
     CDS             35320..35781
                     /gene="hycA"
                     /locus_tag="ACHOSM_RS02270"
                     /old_locus_tag="pgaptmp_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HGU4557045.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKEGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            35906..36517
                     /gene="hycB"
                     /locus_tag="ACHOSM_RS02275"
                     /old_locus_tag="pgaptmp_000455"
     CDS             35906..36517
                     /gene="hycB"
                     /locus_tag="ACHOSM_RS02275"
                     /old_locus_tag="pgaptmp_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HGU4557046.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            36514..38340
                     /gene="hycC"
                     /locus_tag="ACHOSM_RS02280"
                     /old_locus_tag="pgaptmp_000456"
     CDS             36514..38340
                     /gene="hycC"
                     /locus_tag="ACHOSM_RS02280"
                     /old_locus_tag="pgaptmp_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HGU4557047.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRP
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     GIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQVFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            38343..39266
                     /gene="hycD"
                     /locus_tag="ACHOSM_RS02285"
                     /old_locus_tag="pgaptmp_000457"
     CDS             38343..39266
                     /gene="hycD"
                     /locus_tag="ACHOSM_RS02285"
                     /old_locus_tag="pgaptmp_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HGU4557048.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDISPRITWAGFGFAFLAFVSLLAA"
     gene            39284..40993
                     /gene="hycE"
                     /locus_tag="ACHOSM_RS02290"
                     /old_locus_tag="pgaptmp_000458"
     CDS             39284..40993
                     /gene="hycE"
                     /locus_tag="ACHOSM_RS02290"
                     /old_locus_tag="pgaptmp_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HGU4557049.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            41003..41545
                     /gene="hycF"
                     /locus_tag="ACHOSM_RS02295"
                     /old_locus_tag="pgaptmp_000459"
     CDS             41003..41545
                     /gene="hycF"
                     /locus_tag="ACHOSM_RS02295"
                     /old_locus_tag="pgaptmp_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HGU4557050.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            41545..42312
                     /gene="hycG"
                     /locus_tag="ACHOSM_RS02300"
                     /old_locus_tag="pgaptmp_000460"
     CDS             41545..42312
                     /gene="hycG"
                     /locus_tag="ACHOSM_RS02300"
                     /old_locus_tag="pgaptmp_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HGU4557051.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            42309..42719
                     /gene="hycH"
                     /locus_tag="ACHOSM_RS02305"
                     /old_locus_tag="pgaptmp_000461"
     CDS             42309..42719
                     /gene="hycH"
                     /locus_tag="ACHOSM_RS02305"
                     /old_locus_tag="pgaptmp_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HGU4557052.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            42712..43182
                     /gene="hycI"
                     /locus_tag="ACHOSM_RS02310"
                     /old_locus_tag="pgaptmp_000462"
     CDS             42712..43182
                     /gene="hycI"
                     /locus_tag="ACHOSM_RS02310"
                     /old_locus_tag="pgaptmp_000462"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HGU4557053.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            43207..43980
                     /locus_tag="ACHOSM_RS02315"
                     /old_locus_tag="pgaptmp_000463"
     CDS             43207..43980
                     /locus_tag="ACHOSM_RS02315"
                     /old_locus_tag="pgaptmp_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557054.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            44142..44453
                     /locus_tag="ACHOSM_RS02320"
                     /old_locus_tag="pgaptmp_000464"
     CDS             44142..44453
                     /locus_tag="ACHOSM_RS02320"
                     /old_locus_tag="pgaptmp_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HGU4557055.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            44450..44869
                     /locus_tag="ACHOSM_RS02325"
                     /old_locus_tag="pgaptmp_000465"
     CDS             44450..44869
                     /locus_tag="ACHOSM_RS02325"
                     /old_locus_tag="pgaptmp_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4557056.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(44983..46407)
                     /gene="ascB"
                     /locus_tag="ACHOSM_RS02330"
                     /old_locus_tag="pgaptmp_000466"
     CDS             complement(44983..46407)
                     /gene="ascB"
                     /locus_tag="ACHOSM_RS02330"
                     /old_locus_tag="pgaptmp_000466"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HGU4557057.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDGPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLLASA
                     LATKIANEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(46416..47873)
                     /gene="ascF"
                     /locus_tag="ACHOSM_RS02335"
                     /old_locus_tag="pgaptmp_000467"
     CDS             complement(46416..47873)
                     /gene="ascF"
                     /locus_tag="ACHOSM_RS02335"
                     /old_locus_tag="pgaptmp_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HGU4557058.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            48133..49143
                     /gene="ascG"
                     /locus_tag="ACHOSM_RS02340"
                     /old_locus_tag="pgaptmp_000468"
     CDS             48133..49143
                     /gene="ascG"
                     /locus_tag="ACHOSM_RS02340"
                     /old_locus_tag="pgaptmp_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HGU4557059.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIHRDSLIAL
                     SR"
     gene            49292..49819
                     /gene="hydN"
                     /locus_tag="ACHOSM_RS02345"
                     /old_locus_tag="pgaptmp_000469"
     CDS             49292..49819
                     /gene="hydN"
                     /locus_tag="ACHOSM_RS02345"
                     /old_locus_tag="pgaptmp_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HGU4557060.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            49972..52224
                     /gene="hypF"
                     /locus_tag="ACHOSM_RS02350"
                     /old_locus_tag="pgaptmp_000470"
     CDS             49972..52224
                     /gene="hypF"
                     /locus_tag="ACHOSM_RS02350"
                     /old_locus_tag="pgaptmp_000470"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HGU4557061.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(52352..53485)
                     /gene="norW"
                     /locus_tag="ACHOSM_RS02355"
                     /old_locus_tag="pgaptmp_000471"
     CDS             complement(52352..53485)
                     /gene="norW"
                     /locus_tag="ACHOSM_RS02355"
                     /old_locus_tag="pgaptmp_000471"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HGU4557062.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(53482..54921)
                     /gene="norV"
                     /locus_tag="ACHOSM_RS02360"
                     /old_locus_tag="pgaptmp_000472"
     CDS             complement(53482..54921)
                     /gene="norV"
                     /locus_tag="ACHOSM_RS02360"
                     /old_locus_tag="pgaptmp_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HGU4557063.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            55108..56622
                     /gene="norR"
                     /locus_tag="ACHOSM_RS02365"
                     /old_locus_tag="pgaptmp_000473"
     CDS             55108..56622
                     /gene="norR"
                     /locus_tag="ACHOSM_RS02365"
                     /old_locus_tag="pgaptmp_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HGU4557064.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            complement(56619..57584)
                     /gene="gutQ"
                     /locus_tag="ACHOSM_RS02370"
                     /old_locus_tag="pgaptmp_000474"
     CDS             complement(56619..57584)
                     /gene="gutQ"
                     /locus_tag="ACHOSM_RS02370"
                     /old_locus_tag="pgaptmp_000474"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HGU4557065.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(57577..58350)
                     /gene="srlR"
                     /locus_tag="ACHOSM_RS02375"
                     /old_locus_tag="pgaptmp_000475"
                     /gene_synonym="gutR"
     CDS             complement(57577..58350)
                     /gene="srlR"
                     /locus_tag="ACHOSM_RS02375"
                     /old_locus_tag="pgaptmp_000475"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HGU4557066.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(58417..58776)
                     /gene="gutM"
                     /locus_tag="ACHOSM_RS02380"
                     /old_locus_tag="pgaptmp_000476"
     CDS             complement(58417..58776)
                     /gene="gutM"
                     /locus_tag="ACHOSM_RS02380"
                     /old_locus_tag="pgaptmp_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HGU4557067.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            complement(58881..59660)
                     /gene="srlD"
                     /locus_tag="ACHOSM_RS02385"
                     /old_locus_tag="pgaptmp_000477"
     CDS             complement(58881..59660)
                     /gene="srlD"
                     /locus_tag="ACHOSM_RS02385"
                     /old_locus_tag="pgaptmp_000477"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HGU4557068.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            complement(59664..60035)
                     /gene="srlB"
                     /locus_tag="ACHOSM_RS02390"
                     /old_locus_tag="pgaptmp_000478"
                     /gene_synonym="gutB"
     CDS             complement(59664..60035)
                     /gene="srlB"
                     /locus_tag="ACHOSM_RS02390"
                     /old_locus_tag="pgaptmp_000478"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HGU4557069.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(60046..61005)
                     /gene="srlE"
                     /locus_tag="ACHOSM_RS02395"
                     /old_locus_tag="pgaptmp_000479"
                     /gene_synonym="gutE"
     CDS             complement(60046..61005)
                     /gene="srlE"
                     /locus_tag="ACHOSM_RS02395"
                     /old_locus_tag="pgaptmp_000479"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HGU4557070.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPPLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(61002..61565)
                     /gene="srlA"
                     /locus_tag="ACHOSM_RS02400"
                     /old_locus_tag="pgaptmp_000480"
                     /gene_synonym="gutA"
     CDS             complement(61002..61565)
                     /gene="srlA"
                     /locus_tag="ACHOSM_RS02400"
                     /old_locus_tag="pgaptmp_000480"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HGU4557071.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            61821..62906
                     /gene="mltB"
                     /locus_tag="ACHOSM_RS02405"
                     /old_locus_tag="pgaptmp_000481"
     CDS             61821..62906
                     /gene="mltB"
                     /locus_tag="ACHOSM_RS02405"
                     /old_locus_tag="pgaptmp_000481"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HGU4557072.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            63051..63548
                     /gene="pncC"
                     /locus_tag="ACHOSM_RS02410"
                     /old_locus_tag="pgaptmp_000482"
     CDS             63051..63548
                     /gene="pncC"
                     /locus_tag="ACHOSM_RS02410"
                     /old_locus_tag="pgaptmp_000482"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HGU4557073.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            63628..64689
                     /gene="recA"
                     /locus_tag="ACHOSM_RS02415"
                     /old_locus_tag="pgaptmp_000483"
     CDS             63628..64689
                     /gene="recA"
                     /locus_tag="ACHOSM_RS02415"
                     /old_locus_tag="pgaptmp_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGU4557074.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TLDFSVDDSEGVAETNEDF"
     gene            64932..65432
                     /gene="recX"
                     /locus_tag="ACHOSM_RS02420"
                     /old_locus_tag="pgaptmp_000484"
     CDS             64932..65432
                     /gene="recX"
                     /locus_tag="ACHOSM_RS02420"
                     /old_locus_tag="pgaptmp_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGU4557075.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            65560..68190
                     /gene="alaS"
                     /locus_tag="ACHOSM_RS02425"
                     /old_locus_tag="pgaptmp_000485"
     CDS             65560..68190
                     /gene="alaS"
                     /locus_tag="ACHOSM_RS02425"
                     /old_locus_tag="pgaptmp_000485"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGU4557076.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPYYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            68425..68610
                     /gene="csrA"
                     /locus_tag="ACHOSM_RS02430"
                     /old_locus_tag="pgaptmp_000486"
     CDS             68425..68610
                     /gene="csrA"
                     /locus_tag="ACHOSM_RS02430"
                     /old_locus_tag="pgaptmp_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HGU4557077.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            68926..69018
                     /locus_tag="ACHOSM_RS02435"
                     /old_locus_tag="pgaptmp_000487"
     tRNA            68926..69018
                     /locus_tag="ACHOSM_RS02435"
                     /old_locus_tag="pgaptmp_000487"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:68960..68962,aa:Ser,seq:gct)
     gene            69022..69098
                     /locus_tag="ACHOSM_RS02440"
                     /old_locus_tag="pgaptmp_000488"
     tRNA            69022..69098
                     /locus_tag="ACHOSM_RS02440"
                     /old_locus_tag="pgaptmp_000488"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69056..69058,aa:Arg,seq:acg)
     gene            69296..69372
                     /locus_tag="ACHOSM_RS02445"
                     /old_locus_tag="pgaptmp_000489"
     tRNA            69296..69372
                     /locus_tag="ACHOSM_RS02445"
                     /old_locus_tag="pgaptmp_000489"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69330..69332,aa:Arg,seq:acg)
     gene            69436..69512
                     /locus_tag="ACHOSM_RS02450"
                     /old_locus_tag="pgaptmp_000490"
     tRNA            69436..69512
                     /locus_tag="ACHOSM_RS02450"
                     /old_locus_tag="pgaptmp_000490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69470..69472,aa:Arg,seq:acg)
     gene            69793..70359
                     /gene="yqaB"
                     /locus_tag="ACHOSM_RS02455"
                     /old_locus_tag="pgaptmp_000491"
     CDS             69793..70359
                     /gene="yqaB"
                     /locus_tag="ACHOSM_RS02455"
                     /old_locus_tag="pgaptmp_000491"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HGU4557078.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIGLNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            70356..70784
                     /gene="yqaA"
                     /locus_tag="ACHOSM_RS02460"
                     /old_locus_tag="pgaptmp_000492"
     CDS             70356..70784
                     /gene="yqaA"
                     /locus_tag="ACHOSM_RS02460"
                     /old_locus_tag="pgaptmp_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HGU4557079.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            70857..72413
                     /gene="gshA"
                     /locus_tag="ACHOSM_RS02465"
                     /old_locus_tag="pgaptmp_000493"
     CDS             70857..72413
                     /gene="gshA"
                     /locus_tag="ACHOSM_RS02465"
                     /old_locus_tag="pgaptmp_000493"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HGU4557080.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFKDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            72563..73078
                     /gene="luxS"
                     /locus_tag="ACHOSM_RS02470"
                     /old_locus_tag="pgaptmp_000494"
     CDS             72563..73078
                     /gene="luxS"
                     /locus_tag="ACHOSM_RS02470"
                     /old_locus_tag="pgaptmp_000494"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGU4557081.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(73142..74680)
                     /gene="emrB"
                     /locus_tag="ACHOSM_RS02475"
                     /old_locus_tag="pgaptmp_000495"
     CDS             complement(73142..74680)
                     /gene="emrB"
                     /locus_tag="ACHOSM_RS02475"
                     /old_locus_tag="pgaptmp_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HGU4557082.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(74697..75869)
                     /gene="emrA"
                     /locus_tag="ACHOSM_RS02480"
                     /old_locus_tag="pgaptmp_000496"
     CDS             complement(74697..75869)
                     /gene="emrA"
                     /locus_tag="ACHOSM_RS02480"
                     /old_locus_tag="pgaptmp_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HGU4557083.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(75996..76526)
                     /gene="emrR"
                     /locus_tag="ACHOSM_RS02485"
                     /old_locus_tag="pgaptmp_000497"
     CDS             complement(75996..76526)
                     /gene="emrR"
                     /locus_tag="ACHOSM_RS02485"
                     /old_locus_tag="pgaptmp_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HGU4557084.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(76617..76952)
                     /gene="ygaH"
                     /locus_tag="ACHOSM_RS02490"
                     /old_locus_tag="pgaptmp_000498"
     CDS             complement(76617..76952)
                     /gene="ygaH"
                     /locus_tag="ACHOSM_RS02490"
                     /old_locus_tag="pgaptmp_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HGU4557085.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(76942..77679)
                     /gene="ygaZ"
                     /locus_tag="ACHOSM_RS02495"
                     /old_locus_tag="pgaptmp_000499"
     CDS             complement(76942..77679)
                     /gene="ygaZ"
                     /locus_tag="ACHOSM_RS02495"
                     /old_locus_tag="pgaptmp_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HGU4557086.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWIAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(77803..78987)
                     /locus_tag="ACHOSM_RS02500"
                     /old_locus_tag="pgaptmp_000500"
                     /pseudo
     CDS             complement(77803..78987)
                     /locus_tag="ACHOSM_RS02500"
                     /old_locus_tag="pgaptmp_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(79279..80271)
                     /gene="proX"
                     /locus_tag="ACHOSM_RS02505"
                     /old_locus_tag="pgaptmp_000501"
     CDS             complement(79279..80271)
                     /gene="proX"
                     /locus_tag="ACHOSM_RS02505"
                     /old_locus_tag="pgaptmp_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HGU4557087.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(80329..81393)
                     /gene="proW"
                     /locus_tag="ACHOSM_RS02510"
                     /old_locus_tag="pgaptmp_000502"
     CDS             complement(80329..81393)
                     /gene="proW"
                     /locus_tag="ACHOSM_RS02510"
                     /old_locus_tag="pgaptmp_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HGU4557088.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(81386..82588)
                     /gene="proV"
                     /locus_tag="ACHOSM_RS02515"
                     /old_locus_tag="pgaptmp_000503"
     CDS             complement(81386..82588)
                     /gene="proV"
                     /locus_tag="ACHOSM_RS02515"
                     /old_locus_tag="pgaptmp_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HGU4557089.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(82943..83902)
                     /gene="nrdF"
                     /locus_tag="ACHOSM_RS02520"
                     /old_locus_tag="pgaptmp_000504"
     CDS             complement(82943..83902)
                     /gene="nrdF"
                     /locus_tag="ACHOSM_RS02520"
                     /old_locus_tag="pgaptmp_000504"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU4557090.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(83912..86056)
                     /gene="nrdE"
                     /locus_tag="ACHOSM_RS02525"
                     /old_locus_tag="pgaptmp_000505"
     CDS             complement(83912..86056)
                     /gene="nrdE"
                     /locus_tag="ACHOSM_RS02525"
                     /old_locus_tag="pgaptmp_000505"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU4557091.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFHTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(86029..86439)
                     /gene="nrdI"
                     /locus_tag="ACHOSM_RS02530"
                     /old_locus_tag="pgaptmp_000506"
     CDS             complement(86029..86439)
                     /gene="nrdI"
                     /locus_tag="ACHOSM_RS02530"
                     /old_locus_tag="pgaptmp_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGU4557092.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(86436..86681)
                     /gene="nrdH"
                     /locus_tag="ACHOSM_RS02535"
                     /old_locus_tag="pgaptmp_000507"
     CDS             complement(86436..86681)
                     /gene="nrdH"
                     /locus_tag="ACHOSM_RS02535"
                     /old_locus_tag="pgaptmp_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGU4557093.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(86929..87258)
                     /gene="ygaM"
                     /locus_tag="ACHOSM_RS02540"
                     /old_locus_tag="pgaptmp_000508"
     CDS             complement(86929..87258)
                     /gene="ygaM"
                     /locus_tag="ACHOSM_RS02540"
                     /old_locus_tag="pgaptmp_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HGU4557094.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            87410..87754
                     /gene="ygaC"
                     /locus_tag="ACHOSM_RS02545"
                     /old_locus_tag="pgaptmp_000509"
     CDS             87410..87754
                     /gene="ygaC"
                     /locus_tag="ACHOSM_RS02545"
                     /old_locus_tag="pgaptmp_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HGU4557095.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(87791..88240)
                     /gene="alaE"
                     /locus_tag="ACHOSM_RS02550"
                     /old_locus_tag="pgaptmp_000510"
     CDS             complement(87791..88240)
                     /gene="alaE"
                     /locus_tag="ACHOSM_RS02550"
                     /old_locus_tag="pgaptmp_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HGU4557096.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT        21119 a        22707 c        23951 g        20995 t
ORIGIN      
        1 agatttttac tcagttaaac cacaacctgg tggtttaatc tggcgcgact ggttaggcct
       61 catagaggta ggtaactcaa aaaacaatac tgaactcccc gcacaggtcg tgaaactttt
      121 gaatgccagc aatctaaagc agacaagggt gggcttatgg ggatttggtt ttgattttga
      181 agatatgaag gttcgttgtt ggtatgaaca ccatttcccg ctgttgttaa acaaaaaaga
      241 agacctaata cctaagttgc gtctggcagc gcaagcggca tcacacattc taagcttgtt
      301 acaccgagcg ttgaaagaag catggttctc tgagaagaaa accacaaagc ttgatttcgg
      361 ttttgtggat attgacttct ggaacaaaac tcagcattga ttcctgagat tagtgcgcaa
      421 aattgaagaa ggtcaggatc cggacgaatt actcagtaaa tggcaaaagg aaatgtggtt
      481 attcgcgcgt caggattttg acgatcgtgt ctttaccaat ccatatgagc ccgttgattt
      541 ggaacgcgtc atgaccgcac gcaagaaata ttttacaaca tcagcggaga agcaaaatgc
      601 taacgccgcc agggagaaaa agcaggaggc tgctgaatga gtattgtaaa agatgagcat
      661 aaagttgcgc tgcgtaaatg gcatgaagag ttgcaggata aacgcggcga gcgtgccagt
      721 ctgcggcgta gtacgacggt taatgatgtt tgtctgacgg atggatttcg cctgtttctt
      781 aagaacagac aaataaagtg gcaggatgaa cccgagtggc gtatcaccgc actagcgctg
      841 atagcagcag ttagcgccaa tgttaaagct attgacgaac ggcagccttt tgccgcgcaa
      901 ctggcggcgg taatgaaaaa ggggcggttt actcgtttgt tggcagtaaa aacgccggat
      961 gatttattgc ggcagttacg ccgtgcggtc aaattattaa acggttccgt caatcttgac
     1021 tctctggcag aggggggctt ccgctggtgc caggagagcg atgacctgct caatcatcac
     1081 cgccgccagc agcgaccaac agaatttatc cgtatccgct gggcgctgga atattaccag
     1141 gctggcgacg ccgataacga acaaaattaa taaaggacta tctcatgaca acatttattc
     1201 agcttcattt gttaaccgct taccctgctg ccaatcttaa ccgtgatgat tccggctctc
     1261 cgaaaactgt gattctgggt ggagcaacgc gtctgcgcgt ttcctcgcaa agtctgaagc
     1321 gtgcttggcg cacttctgaa ctttttgaac aggcgctggc gggccatatt ggtgttcgta
     1381 gcgggcgtat tgcgcgtgag gcggcaacta tcctgattga gaaaggaatc gaagataaaa
     1441 aagccatcga atgggcagta gagatagccg attatcttgg gaaagccaaa aaagacaaaa
     1501 aacaaaaaaa cgacaaaaaa ccaaaagatc cgctcactag cgccgaaact gaacagctcg
     1561 tccatatcag cccggcagaa tttgacgccg taaaagcgct ggcccatcaa ctggcagaag
     1621 aaaagcgcgc gccaaaagag aaagatctcg ctttgttacg taaagaccgt atggcggtag
     1681 atatcgctat gtttggtcgt atgctggcga aaaaacccgg gtttaatgta gaagccgcct
     1741 gccaggtcgc gcatgcgttt ggtgtcagtg aaacgattgt cgaaaatgat tttttcaccg
     1801 ccgtagatga tttacgccag gcttctgaag atgccggtgc cggtcatgta gatgaaaccg
     1861 gattcggttc tgcgctgttc tacacctata tctgcattga taaagatctg ctggtagaaa
     1921 acctcggcgg tgacgaagcg ttagctaatc agaccatacg cgcctttacg gaagccgcgc
     1981 ttaaagtctc cccaaccggt aaacaaaaca gctttgccag ccgggcttat gcctcctggg
     2041 cgctggcaga aaaaggcacc gatcaaccgc gttctctggc ggcagctttc tatgaaccca
     2101 ttaatggcac ccgtcagtta gaggtggcgg tgcagcgtat tacaactctt cgcgaaaata
     2161 tgaatacggt ctacgaacag aagaccgatt acgcaagttt tgacgtgatg aacaaacagg
     2221 gaagcatgaa ggacgtgctg gactttatct gcgcgtaagg ggcggcttat gagccaatat
     2281 ctgatttttc agcttcatgg gccgatggca tcctggggcg tcgatgcccc cggcgaagtg
     2341 cgtcataccc atgaactgcc ttcgcgctca gcattgctgg ggctgctggc tgccggggta
     2401 gggatccggc gtgatgatac cgaacgctta aacgcgttta atcgccacta ttcactggtg
     2461 gtttgcgcca gccgtaaccc gcgctgggca cgggattatc acacggtcca gatgccaaaa
     2521 gaggtgcgta aagcacgtta tttcagccgt cgcgaagagt tgagcgaccc tgagcttttg
     2581 agcgcgatta tctcccggcg cgactactac accgatgcct ggtggatggt ggcggtggca
     2641 acaacccccg atgcgcctta cagcctcgaa caattgcagg acggtttacg ccatccggtt
     2701 tttccgcttt atctggggcg aaaaagtcat ccgctggcgt taccacttgc gccgttacta
     2761 ctcgaaggca acgcgtcgga taccttacgt aacgcatacc aacagtatca ggatcatttc
     2821 cgcaaactga aagtctcact cccaaaactt caggatgaat gctggtggga aggggaacac
     2881 gacggcctgg tcgcgagcaa aatattacgc cgtcgggatg ttcctttaaa tcgtcagcag
     2941 tggttgtttg gggaacgcac cgtcaatcag gggccgtggc tcagcaagga ggaaccatgt
     3001 acctctcaag aataacctta cataccggcc agctttcccc agcgcagttg ctgcatctgg
     3061 tggatcgcgg tgaatatgtg atgcatcagt ggctgtggga tctcttccct ggcggcaaag
     3121 aaaggcaatt tctttatcgt cgggaagaac tccagggcgc gtttcgcttt tttgtcctct
     3181 cgcaggaacg cccggcggaa agtgaaactt tcaccatcga atgccgacca tttgtgtccg
     3241 agctgcgtac cggacaatct ctttgtttca acctgcgggc gaatccaaca atctgtaaag
     3301 cgggcaagcg ccacgatctg ctgatggagg cgaaacggca ggtgaagggg caggcggaag
     3361 ggcgtgatat ctggttgcat caacaacagg cggcgctgga ctggctggca gcgcaggggg
     3421 aaagaagtgg ttttacactg ctggatactt cggttgatgc ctaccgacaa cagcaactgc
     3481 ggcgggaaaa ctcccggcaa ctgatccagt tcagtagcgt ggattatacg gggatgctta
     3541 cggtcaccgc tcctgggtta tttctgcaac gtctcagtca ggggtatggc aaaagccggg
     3601 cgttcggttg tgctctgatg ctgatcaaac ccggagcaga ggcgtgacgt ttgtaccgct
     3661 gagcccgatc ccattaaaag atcgcacttc catgatcttc ctccagtacg gtcaaatcga
     3721 cgtactggac ggcgctttcg tgctgatcga caaaaccggg atccgcacgc atattccagt
     3781 gggatcggtc gcctgcatta tgctcgaacc gggaacaaga gtttcccacg cggcagtcca
     3841 tctggccgcc acggtgggaa cactgctggt ctgggtcggt gaagcgggcg ttcgcgttta
     3901 ctcctccgga caacccggag gggcgcgggc agataaatta ctctaccagg caaagctggc
     3961 tttaacggaa gatctacggt tgaaggtggt gcgcaaaatg tatgaattac gttttcgtga
     4021 accaccgcca gctcgccgtt cagtggagca gttacgtgga attgagggat ctcgcgttcg
     4081 ccagacgtat gcattactgg cgaaacaata tggtgtgaaa tggaatggtc gcaaatacga
     4141 tcctaaagac tgggaaaaag gcgatgttgt gaatcgctgc atcagtgctg ctacatcatg
     4201 tctgtacggt atatctgaag cggcagtatt agccgcggga tatgcgcccg ctattgggtt
     4261 tatccatagt ggcaaaccgc tttcatttgt ttatgacata gccgatatca ttaaatttga
     4321 ttcggttgtg ccaaaggcat ttgaaatagc agcgaggcaa cccgcagaac ctgataaaga
     4381 agtcagatta gcctgtcgcg atattttccg tggcactaag ttaacgggca aattaatacc
     4441 gttaattgag gaagtccttg ctgcaggtga aattgaacca ccacaacccg cgccggatat
     4501 gttaccgcct gccatccctg aacctgaaac gctgggtgat agtggtcact gggggcgcgg
     4561 cggatgagca tggtcgtggt tgttacagaa aatgtccctc cgcgcttacg tggacggctc
     4621 gcaatctggc tactggaagt gcgtgccggt gtgtatgttg gtgatacatc aaaacgtatt
     4681 cgggagatga tctggcagca aattacccaa ctggctggtt gcggaaatgt ggtgatggcc
     4741 tgggcgacca ataccgagtc gggttttgaa tttcagacct ggggagaaaa cagacgtatt
     4801 ccggtggatt tggatgggtt acgtttggtt tcttttcttc ctgttgataa tcaataagtt
     4861 agacgttctt taaaaataag gaaatgtttt aatttagttg gtagattgtt gatgcggaat
     4921 aaatttgttt aaaaacagtt atgtatgctt agtgtgttcc ccgcgccagc ggggataaac
     4981 cgttaattcc tcgtttcccc ggattccctc ggggcgtgtt ccccgcgcca gcggggataa
     5041 accgtattgt ctgggataaa tgctggattg gtactggtgt tccccgcgcc agcggggata
     5101 aaccgaatag cgggtacggc tgaaaagtgg attttacgtg ttccccgcgc cagcggggat
     5161 aaaccgggcg cactggatgc gatgatggat atcacttaga attccccgcc cctgcggtag
     5221 aacacccagc tcccattttc aaacccatca agacgccttc gccagctcct tcaccaacgg
     5281 tagcattatc cgcataacgt cacggcagcg acgttctatt cttccaggaa gtgccttatc
     5341 aatatgctgt tgattatcca gtcttacgtc atgccagcta tttcccgcag ggaatgcggc
     5401 tgtttttgcg cgttgctgat aaccatcctt attcccaaga ttccagttag tcgcttccac
     5461 cgaaagtaca gcaatgcccg ctttgtcgaa tatttctgcg tcattacaac acccagtgcc
     5521 tttcggataa tttttattca aacccggatt ggtcgttgcg gctattccgt gactgcgcgc
     5581 aattgccagc gccctgtcgc gcgttaattt ccttactgct tcaggggttt ttacaccgct
     5641 gttgaaatac aatttatcgc caacaattaa gttatcgaga ttaatcacca gcagcgtatt
     5701 tttcttttcg gtgtcactca tccgcttgag taaattctca gcgcctaatt tcccttcctc
     5761 ttcgccgctg gtcgccacaa atcgaatacc atactcggta ggcgtatttt tcaggcgttc
     5821 tgccaattcc agcatgacac ctaaacctgc ggcgttatca tccattcctt gtaacgtaag
     5881 cccgccgaga ttggcatcgg cgtcagcatc gctcagcggg gcgtaagtat ccagatgcgc
     5941 cataatgatg atctgctgcg gcgctttgcc ttcatgagcg gcaatcaccg tacttcctgt
     6001 cacgttatgc cagttcttgc gattatcgcg ggcggtataa atataccgac tattaaatgt
     6061 ccgaatatca ctgcgataac ccatttgctg aaactgttgg cgaatataat cggcagataa
     6121 catttctgca ggagttccgg tcatgcgtcc cggaaagaaa gtagcaatat gtcgtgcctg
     6181 agtattagca aaatcgccag gtttaggtga cgaggcgtgt acggggagaa taaagcatac
     6241 gccgagcgcc agggcagcgg tacggtggcg caatgcggaa aacatagtga gtccttaaat
     6301 accatgcaaa tttttttacc gccatagtat gaaactgccg ctgcgctaaa acaatttcaa
     6361 atcttcctaa acgccagaaa tccggtgcct taagcacttt ttgatattag ctttgccaaa
     6421 tcgttattcc gttaaggaac tactcattct aattggtaat ttcattcgtt ctcttacgct
     6481 ccctatagtc gaaacatctg atggcaagaa aatagcggta ttgcaaagga acggttatgg
     6541 atcaaatacg acttactcac ctgcggcaac tggaggcgga aagcatccac attattcgcg
     6601 aggtggcggc agaattctca aatccggtga tgctctactc tatcggtaaa gattccagcg
     6661 tcatgctgca tttggcgcgc aaggcgtttt atccaggtac gctgcctttc ccgttgctgc
     6721 atgtcgatac cggctggaaa ttccgcgaga tgtatgagtt ccgcgatcgt actgctaaag
     6781 cctacggctg cgagctgctg gtgcataaaa acccggaagt cgtggcgatg gggattaatc
     6841 cattcataca cggcagcgcg aaacataccg atattatgaa aactgaaggc ctgaaacagg
     6901 ccctgaataa atacggtttt gatgccgcct tcggtggtgc gcgccgtgat gaagagaaat
     6961 cccgcgctaa agagcgaatc tactctttcc gtgaccgctt ccatcgctgg gatccgaaaa
     7021 atcagcgccc ggagctgtgg cacaactaca acgggcaaat taacaaaggt gaaagcatcc
     7081 gcgtcttccc gctctctaac tggaccgagc aggatatctg gcaatacatc tggctggaaa
     7141 atatcgacat tgtgccgctg tatctcgctg cggaacgtcc ggttctggaa cgcgacggta
     7201 tgttgatgat gattgatgac aaccgtatcg acctgcaacc gggcgaagtg atcaaaaaac
     7261 ggatggtgcg tttccgtacg ctgggctgct ggccgctgac cggtgcggtg gagtcaaatg
     7321 cacaaacact gccggaaatc atcgaagaga tgctggtttc caccaccagt gaacgtcagg
     7381 gccgcgtgat tgaccgcgac caggcggggt ctatggagct gaaaaaacgt caggggtatt
     7441 tttaagatga acaccgcact tgcacaacaa atcgccaatg aaggcggcgt cgaagcctgg
     7501 atgattgcgc aacaacataa aagcctgctg cgttttctga cctgtggcag cgtcgatgac
     7561 ggcaaaagca ctctgattgg tcgcctgctg cacgatactc gccaaatcta tgaagatcag
     7621 ctctcatcgc tgcataacga cagtaagcgt cacggtaccc agggcgaaaa gctggatctg
     7681 gctctgttgg tggacggcct gcaagctgag cgcgaacagg gcatcactat tgatgtggcc
     7741 taccgctatt tctctaccga gaagcgtaaa tttattatcg ccgacacccc agggcacgag
     7801 cagtacactc gcaatatggc gactggcgca tcgacatgtg aactggcgat cttactgatc
     7861 gatgcccgta aaggcgtgct cgatcaaacc cgtcgtcaca gttttatctc cacactgttg
     7921 gggatcaaac atctggtcgt ggcgatcaac aaaatggatc tggtggatta cagtgaagag
     7981 acgttcaccc gtattcgtga agattatctg acctttgccg ggcagctgcc gggtaatctg
     8041 gatatccgct ttgtgccgct ctctgcactg gaaggcgaca acgtggcatc gcaaagtgaa
     8101 agtatgccgt ggtacagcgg tccgacactg ctcgaagtgc tggaaaccgt ggagatccag
     8161 cgagtggtgg atgctcagcc aatgcgcttc ccggtgcagt acgttaatcg cccgaatctc
     8221 gattttcgtg gctacgccgg aacgctggca tccggtcgcg tggaagtcgg gcaacgtgta
     8281 aaagtgctgc cctctggtgt ggaatcaaac gtcgcgcgga tcgtgacttt tgatggtgat
     8341 cgcgaagaag cctttgccgg agaagcgatc accctggtgc tgacggatga gatcgacatc
     8401 agccgtggcg atctgctgct ggcggcagac gaagcgttac cggcggtgca gagcgcgtcg
     8461 gtggatgtgg tatggatggc ggaacagccg ctttctccag ggcagagtta cgacatcaaa
     8521 attgccggta agaagacgcg cgcgcgtgtt gatggcattc gctatcaggt tgatattaat
     8581 aaccttaccc agcgtgaagt tgaaaacctg ccactgaatg ggatcggcct cgtggatctc
     8641 acttttgacg agccgctggt gttagatcgt tatcaacaaa atccggtgac gggtgggctg
     8701 atttttatcg atcgcctgag caatgtgacc gtgggtgccg gtatggtgca cgagccagtt
     8761 agccaggcaa ctgctgcgcc atctgaattc agtgcattcg aactggaatt gaatgctctg
     8821 gttcgtcgcc actttccgca ctggggcgcg cgcgatttgc tgggggataa ataatggcgc
     8881 tgcatgacga aaacgtcgtc tggcatagcc atccggtcac tgtgcaacaa cgcgagctac
     8941 accacggtca tcgtggtgta gtgctgtggt ttaccggcct ctccgggtcc ggtaaatcaa
     9001 cggtcgccgg ggcgctggag gaggcgttac ataaactcgg cgtcagtacg tatctgctgg
     9061 atggcgacaa tgttcgccac ggattatgca gcgatctcgg ttttagcgat gccgatcgta
     9121 aagagaatat ccgtcgcgtc ggtgaagtgg cgaatttgat ggttgaagcc ggactggtgg
     9181 tgctgaccgc atttatctcg ccacaccgcg ccgaacgcca gatggttcgc gaacgcgtag
     9241 gagaagggcg ctttatcgaa gtgtttgtcg atacgccgct ggcgatttgc gaagcccgcg
     9301 atcccaaagg cttatataag aaagcgcgtg ccggtgaact gcgcaacttt acgggaatag
     9361 attccgttta cgaagcgcct gaatcggcag aaattcatct caatggtgaa caattagtaa
     9421 caaatttggt acagcaatta ttagatctgt tgagacagaa cgatattatc agatcctgag
     9481 acaccaccgg gtttccatgc ccggttcgtc aaagtaacag gatcaaatat gcgtaatagc
     9541 cataacatta cactaacaaa taacgacagc cttactgagg atgaagaaac cacatggtca
     9601 ctgcctggtg ccgtggttgg ttttatctcc tggttatttg cgctggcgat gccgatgttg
     9661 atttatggct ctaacacgct gttcttcttt atctacacct ggcctttctt tctggcactg
     9721 atgcctgtcg cggtagtggt ggggattgcg ctgcattcat tgatggacgg aaagctacgc
     9781 tacagtattg ttttcaccct agtgacagta ggcattatgt ttggcgcact gtttatgtgg
     9841 ctactgggct aatttgtact ttcccgtctc ctctgttcat aattcaaacc gtaactaata
     9901 atgagattat gttctgcacg ccctgggtat acgtaacaat ggacaaatgt ggtacatttg
     9961 cccgcgttgt cgcggtatcc ccaacagagg atgtagagtc gtcttcggat gcatgggatg
    10021 atgatgccgt ttttcagggg gcaggatggg taaactaacg ctgctgttgc tggctattct
    10081 ggtctggcta cagtattcgc tgtggttcgg taagaacggt atacatgact atacccgcgt
    10141 caatgatgat gtggcggcac agcaagctac aaacgcgaaa cttaaagcgc gaaacgatca
    10201 actttttgcc gaaattgacg atctcaatgg cggccaggag gcgctcgaag agcgtgcgcg
    10261 taatgaactc agcatgacca ggccgggcga aactttttat cgtctggtgc ctgacgcgtc
    10321 gaagcgcgca cagtctgcgg ggcaaaacaa tcgataaatc agcccgggaa ttaacatggc
    10381 aaccactcat ttggatgttt gcgccgtggt tccggcggcc ggatttggcc gtcgaatgca
    10441 aacggaatgt cctaagcaat atctctcaat cggtaatcaa accattcttg aacactcggt
    10501 gcatgcgctg ctggcgcatc cccgggtgaa acgtgtcgtc attgccataa gtcctggcga
    10561 tagccgtttt gcacaacttc ctctggcgaa tcatccgcaa atcaccgttg tagatggcgg
    10621 tgatgagcgt gccgattccg tgctggcagg tttgaaagcc gctggcgacg cgcagtgggt
    10681 attggtgcat gacgccgctc gtccttgtct gcatcaggat gacctcgcgc gattgttggc
    10741 gttgagcgaa accagccgca cgggagggat cctagccgta ccagtgcgcg atactatgaa
    10801 acgtgccgaa ccgggcaaaa atgccattgc tcataccgtt gatcgcaacg gcttatggca
    10861 cgcgctgacg ccgcaatttt tccctcgtga gctgttacat gactgtctga cgcgcgctct
    10921 aaatgaaggc gcgactatta ctgacgaagc ctcggcgctg gaatattgcg gatttcatcc
    10981 gcagttggtc gaaggccgcg cggataacat taaagtcacg cgcccggaag atttggcact
    11041 ggccgagttt tacctcaccc gaaccatcca tcaggagaat acataatgcg aattggacac
    11101 ggttttgacg tacacgcctt tggcggtgaa ggcccaatta tcattggtgg cgtacgcatt
    11161 ccttacgaaa aaggattgct ggcgcattct gatggcgacg tggcgctcca tgcgttgacc
    11221 gatgcattgc ttggcgcggc ggcgctgggg gatatcggca aactgttccc ggataccgat
    11281 ccggcattta aaggtgccga tagccgcgag ctgctacgcg aagcctggcg tcgtattcag
    11341 gcgaagggtt atacccttgg caacgtcgat gtcactatca tcgctcaggc accgaagatg
    11401 ttgccgcaca ttccacaaat gcgcgtgttt attgccgaag atctcggctg ccatatggat
    11461 gatgttaacg tgaaagccac tactacggaa aaactgggat ttaccggacg tggggaaggg
    11521 attgcctgtg aagcggtggc gctactcatt aaggcaacaa aatgattgag tttgataatc
    11581 tcacttacct ccacggtaaa ccgcaaggca ccgggctgct gaaagccaat ccggaagact
    11641 ttgtggtggt ggaagatttg ggctttgagc ctgatggtga aggtgagcat attctggtta
    11701 gaatcctcaa aaacggctgc aatacccgtt ttgtggcgga tgcactggcg aaattcctga
    11761 aaattcatgc ccgtgaagtc agcttcgctg ggcaaaaaga caaacatgct gttacggaac
    11821 agtggttatg cgctcgcgtg ccgggcaagg aaatgcccga tctgagcgcc tttcaactgg
    11881 aaggctgcca ggtgctggag tatgcgcggc acaagcgcaa gctgcgttta ggggcgctga
    11941 aaggtaacgc ctttaccctg gtactacgcg aagtgagcaa tcgcgatgac gttgaacaac
    12001 gtctgatcga tatttgcgta aaaggtgtac cgaactactt cggtgcccaa cgttttggga
    12061 ttggcggtag caacttgcag ggcgcgctgc gctgggcgca aaccaatact ccggtgcgcg
    12121 atcgcaataa acggagtttt tggttgtcgg cagcccgcag tgcgttgttt aatcagattg
    12181 ttgctgagcg cctcaaaaaa gcagacgtta atcaagttgt tgacggtgat gcgctacaat
    12241 tagccggacg tggtagctgg tttgtcgcaa ccaccgaaga actggcggaa ttacagcgtc
    12301 gcgtcaacga taaagagctg atgataaccg ccgcattgcc gggcagtggc gaatggggaa
    12361 ctcagcgtga agcgctggca ttcgaacaag cagctgtcgc cgcagaaact gaattacaaa
    12421 ccttactggt gcgcgaaaaa gttgaagccg cgcgcagagc gatgctgctg tatccgcaac
    12481 aattaagctg gaattggtgg gatgacgtca ccgtagagat ccgtttctgg cttccggcgg
    12541 gtagttttgc aaccagcgtt gtcagggaac ttatcaacac aacaggtgat tatgcgcata
    12601 ttgctgagta atgatgacgg ggtacatgca cccggtatac aaacgctggc gaaagccttg
    12661 cgtgagtttg ctgacgttca ggtggtcgcc cccgatcgta accgcagcgg cgcttcaaat
    12721 tctctgacac tggaatcctc cctgcgcacg tttacctttg aaaatggtga tattgctgtg
    12781 caaatgggaa ccccgaccga ttgcgtctat cttggcgtga atgctctgat gcgtccgcgc
    12841 ccggacattg ttgtgtccgg aattaacgcc gggccgaatc tgggggatga tgttatttat
    12901 tccggtacgg tagccgccgc gatggaaggc cgtcatttag gttttccggc gcttgccgtc
    12961 tcgcttgacg ggcataaaca ttacgacact gccgcggcgg taacctgttc aattttgcgc
    13021 gcactgtgta aagagccgtt gcgcaccggg cgtattctta atattaacgt tccggattta
    13081 cccttggatc aaatcaaagg tattcgcgtg acgcgctgcg gtacacgaca tccggcagat
    13141 caggtgatcc cgcagcaaga tccgcgcggc aatacgctgt actggattgg cccgccgggc
    13201 ggtaaatgtg atgccggtcc ggggaccgat tttgctgcgg tagatgaggg ctatgtctcc
    13261 atcacgccgc tgcatgtgga tttaactgcg catagcgcgc aagatgtggt ttcagactgg
    13321 ttaaacagcg tgggagttgg cacgcaatgg taagcagacg cgtacaagca cttctggatc
    13381 aattacgtgc gcaaggtatt caggatgagc aggtgctgaa tgcacttgcc gccgtgccgc
    13441 gtgaaaaatt cgttgatgaa gcgtttgaac aaaaagcctg ggacaatatc gccttgccga
    13501 taggtcaggg gcagacaatt tcgcagccgt atatggtggc gcgaatgacc gaattactcg
    13561 agctgacgcc gcagtcgcgg gtgctggaaa ttggcactgg ttcgggatat caaacggcaa
    13621 tcctggcgca tcttgtccag catgtttgct cggtcgaacg gattaaaggt ttgcagtggc
    13681 aggcgcgtcg ccgcctgaaa aatctggatt tacataatgt ttcaacccgt catggcgatg
    13741 gatggcaagg ttggcaggca cgtgcgccgt ttgacgctat cattgttacg gcggcaccgc
    13801 cggaaattcc aactgcgcta atgacgcagc tggacgaagg cgggattctt gtcttacccg
    13861 taggggagga gcaccagtat ttgaaacggg tgcgtcgtcg gggaggcgaa tttattatcg
    13921 ataccgtgga ggccgtgcgc tttgtccctt tagtgaaggg tgagctggct taaaacgtga
    13981 ggaaatacct ggatttttcc tggttatttt gccgcaggtc agcgtatcgt gaacatcttt
    14041 tccagtgttc agtagggtgc cttgcacggt aattatgtca ctggttatta accaattttt
    14101 cctgggggat aaatgagcgc gggaagccca aaattcaccg ttcgccgcat tgcggctttg
    14161 tcactggttt cgctatggct ggcaggctgt tctgacactt caaatccacc ggcaccggtc
    14221 agctccgtta atggcaatgc gcctgcaaat accaattctg gtatgttgat tacgccgccg
    14281 ccgaaaatgg ggacgacgtc tacagcgcag caaccgcaaa ttcagccggt acagcagcca
    14341 caaattcagg ctactcaaca atcgcaaatc cagccagtgc agccagtagc tcagcagccg
    14401 gtacagatgg aaaacggacg catcgtctat aaccgtcagt atgggaacat tccgaaaggc
    14461 agttatagcg gcagtaccta taccgtgaaa aaaggcgaca cacttttcta tatcgcctgg
    14521 attactggca acgatttccg tgaccttgct cagcgcaaca atattcaggc accatacgcg
    14581 ctgaacgtcg gtcagacctt acaggtgggg aatgcttccg gtacgccaat cactggcgga
    14641 aatgccatta cccaggccga cgcagcagag caaggagttg tgatcaagcc tgcacaaaat
    14701 tccaccgttg ctgttgcgtc gcaaccgaca attacgtatt ctgagtcttc gggtgaacag
    14761 agtgctaaca aaatgttgcc gaacaacaag ccaactgcga ccacggtcac agcgcctgta
    14821 acggtaccaa cagcaagcac aaccgagccg actgtcagca gtacatcaac cagtacgcct
    14881 atctccacct ggcgctggcc gactgagggc aaagtgatcg aaacctttgg cgcttctgag
    14941 gggggcaaca aggggattga tatcgcaggc agcaaaggac aggcaattat cgcgaccgca
    15001 gatggccgcg ttgtttatgc cggtaacgcg ctgcgcggct acggtaatct gattatcatc
    15061 aaacataatg atgattacct gagtgcctac gcccataacg acacaatgct ggtccgggaa
    15121 caacaagaag tgaaggcggg gcaaaaaata gcaaccatgg gtagcaccgg aaccagttca
    15181 acacgcttgc attttgaaat tcgttacaag gggaaatccg taaacccgct gcgttatttg
    15241 ccgcagcgat aaatcggcgg aaccaggctt ttgcttgaat gttccgtcaa gggatcacgg
    15301 gtaggagcca ccttatgagt cagaatacgc tgaaagttca tgatttaaat gaagatgcgg
    15361 aatttgatga gaacggagtt gaggtttttg acgaaaaggc cttagtagaa gaggaaccca
    15421 gtgataacga tttggccgaa gaggaactgt tatcgcaggg agccacacag cgtgtgttgg
    15481 acgcgactca gctttacctt ggtgagattg gttattcacc actgttaacg gccgaagaag
    15541 aagtttattt tgcgcgtcgc gcactgcgtg gagatgtcgc ctctcgccgc cggatgatcg
    15601 agagtaactt gcgtctggtg gtaaaaattg cccgccgtta tggcaatcgt ggtctggcgt
    15661 tgctggacct tatcgaagag ggcaacctgg ggctgatccg cgcggtagag aagtttgacc
    15721 cggaacgtgg tttccgcttc tcaacatacg caacctggtg gattcgccag acgattgaac
    15781 gggcgattat gaaccaaacc cgtactattc gtttgccgat tcacatcgta aaggagctga
    15841 acgtttacct gcgaaccgca cgtgagttgt cccataagct ggaccatgaa ccaagtgcgg
    15901 aagagatcgc agagcaactg gataagccag ttgatgacgt cagccgtatg cttcgtctta
    15961 acgagcgcat tacctcggta gacaccccgc tgggtggtga ttccgaaaaa gcgttgctgg
    16021 acatcctggc cgatgaaaaa gagaacggtc cggaagatac cacgcaagat gacgatatga
    16081 agcagagcat cgtcaaatgg ctgttcgagc tgaacgccaa acagcgtgaa gtgctggcac
    16141 gtcgattcgg tttgctgggg tacgaagcgg caacactgga agatgtaggt cgtgaaattg
    16201 gcctcacccg tgaacgtgtt cgccagattc aggttgaagg cctgcgccgt ttgcgcgaaa
    16261 tcctacaaac gcaggggctg aatatcgaag cgctgttccg cgagtaagta agcatctgtc
    16321 agaaaggcca gtctcaagcg aggctggcct tttttctttc ggttggtgag tcggtgtatt
    16381 tgtagtctgg ataagacgcg atagcgtcgc atccggaaat ttgcccccta tgcgttcatc
    16441 atcttgcgca ccagctgcat aaacagctcc tgctcttctc cactcaaacg ccccaaaaat
    16501 tcctcatcca cgctgtcacc aataggtatc gccgcagcaa gtagcttttc tccttcggca
    16561 gtcagccaga caaagcgtcg ccgcttgtca gcgacgtcat gttcacgtct gactaagcca
    16621 cgattttcca ttcttgccaa catttctgct agcgttgctt tggtgctgac tgctgcttct
    16681 atcagtgcca cctgttcaat accaggttta tcagcaatgg cgcgcatcac cgcgtactgt
    16741 ggtttggtca ggtcaggtaa ctcgtgctgc cagcgagccg tgtgctgctg aaaaagctgt
    16801 cgtaactgat ggaacgcttt atttcgtaac gccatgtaaa ctccgggcta attctctgtc
    16861 gctatcatag cggtttttta gtgacgaaga agatgttgtt acttttcaat ccttgccgct
    16921 gtggaaatga aggagtatgt taataataac gttcgtatac gaacaattaa gaggataagc
    16981 aatgaaactg atcgtcggga tgacaggggc taccggtgcg cctcttggtg tggcattact
    17041 gcaagcgctg cgggagatgc cgaatgtcga gactcatctg gtgatgtcga agtgggcgaa
    17101 aaccaccatt gaactggaaa cgccttacag cgctcgcgat gttgctgccc tcgcagactt
    17161 cagccataac ccggcggatc aggcggcgat catctcatcc ggttcttttc gtaccgacgg
    17221 catgatcgtt attccgtgca gtatgaaaac gctcgccggt atccgcgctg gttacgccga
    17281 tggcctggta gggcgcgcgg cggacgtcgt gctcaaagaa ggccgcaaac tggtgctggt
    17341 gccgcgtgaa atgccgctta gcaccatcca tctcgaaaat atgctcgcac tttcacgcat
    17401 gggcgtggcg atggtgccgc cgatgcctgc cttttataac catcccgaaa cggtagatga
    17461 cattgtccac catgtggtag cccgcgtgct ggatcaattt ggcctcgaac atccccatac
    17521 ctggcgctgg caaggattgc cgcaggcccg gaatttttct caggagaatg aataatggca
    17581 tttgatgatt tacgcagctt tttacaggcg cttgatgacc acggccagtt actgaaaatc
    17641 agcgaagagg tgaacgctga gccggatctg gcagcagcag ctaacgccac cgggcgtatc
    17701 ggcgacggcg cgcccgcgct gtggtttgat aatattcgcg gctttaccga tgcccgcgtg
    17761 gcgatgaata ccatcggttc ctggcagaac cacgcgattt cccttggcct gccaccaaac
    17821 accccggtca aaaagcagat tgatgagttt atccgccgct gggataactt cccgattgcc
    17881 ccggagcgcc gcgccaatcc agcctgggcg cagaacaccg tcgatggcga agagattaac
    17941 ctgttcgata tcctgccgct gtttcgttta aacgatggcg atggcggttt ctatctcgac
    18001 aaagcctgcg tggtttcccg cgatccgctc gacccagata acttcggcaa gcagaacgtc
    18061 ggcatctacc gcatggaagt gaagggcaag cgtaagctcg gcctgcaacc ggtgccgatg
    18121 cacgatatcg ccctgcatct gcataaagca gaagagcgcg gtgaagatct gccgattgcg
    18181 atcacgctcg gtaacgatcc gatcatcacg ctgatggggg ccacgccgct gaaatacgat
    18241 cagtctgagt atgaaatggc aggcgcgctg cgtgaaagcc cgtacccgat cgccaccgcc
    18301 ccgctgaccg gttttgatgt gccgtggggt tcagaagtga tcctcgaagg ggttatcgaa
    18361 agccgtaaac gcgaaatcga agggccgttc ggtgagttta ccgggcacta ctccggtggg
    18421 cgcaacatga ccgtggtgcg catcgataaa gtctcttatc gcagcaaacc gattttcgaa
    18481 tcactctatc tcggtatgcc gtggaccgaa atcgactacc tgatggggcc agccacctgc
    18541 gtaccgctgt atcaacaact gaaagccgag ttcccggaag tgcaggcggt aaatgccatg
    18601 tacacccacg gcctgctggc gattatctcc accaaaaaac gctacggcgg ctttgcccgc
    18661 gcggtgggcc tgcgtgcgat gaccacgccg cacggtctgg gctacgtaaa gatggtgatt
    18721 atggtcgatg aagacgttga cccgttcaac ctgccgcagg tgatgtgggc gctctcctcg
    18781 aaagtaaacc cggcagggga tttggtgcag ttgccgaata tgtccgtgct ggaactcgac
    18841 cctggctcaa gcccggcggg gatcaccgac aagctgatta tcgacgctac cacgcctgtc
    18901 gccccggaca accgtggtca ctacagccag ccggtggtgg atttgccgga aaccaaagcc
    18961 tgggctgaaa aactgaccac tatgctggct gcacgtaaat aaggagaaca agatgatttg
    19021 tccacgttgt gccgatgaac agattgaagt gatggtgaaa tcgccggtga aagatgtctg
    19081 gacggtatat cagtgccagc attgccttta tacctggcgc gataccgaac cgctacgccg
    19141 caccagtcgc gaacattacc cggaagcgtt ccgcatgacg cagaaagata ttgatgatgc
    19201 gccaatggtg ccgagcatcc cgccgctgct ggcggaaggt aagcgctaaa taaaaaggtc
    19261 cgatgcccat cggacctttt tattaaggtc aaattaccgc ccatacgcac caggtaatta
    19321 agaatccggt aaaaccgaga atggtcgtta acactgtcca ggttttcaga ccgtctgcta
    19381 ccgacaaccc cagatatttg gtcacaatcc agaaccctga gtcattaata tgtgacgcgc
    19441 caagcccacc aaagcaggct gccagcgtca ccaatacgca ctgaatcgga ttcaatccca
    19501 tcaccgcttc tgagagtaac ccgccggttg tcaggattgc tacggttgct gacccctgcg
    19561 atgcacgcag cgccagtgaa ataataaatg cggctggtaa cagaggcagg tcaatcattt
    19621 gtagcatgtt ggcaagggct ttgccgacgc ccgattccac cagcactttg ccaaataccc
    19681 ctccagcacc agtaaccaaa atcactaccg ccgcagtggg aagggctgag cccataatgt
    19741 cgctggtgtg ttgtaagctc cagccgcgac gtaaagccaa taaccagaat gccagcacca
    19801 gcgcaatcat tagagctacc attggtgagc cgatcagctg tagcgtacca agcaggggat
    19861 gcgaaggcgg catcagtgtt gcggaaaccg tacccgccat gataatcgcg ataggaataa
    19921 caattagcga ggtgaccagc gcgacgcccg gtggatttat tttatcgctt aattttgtcg
    19981 cgccttcctc actggccgga gccagttgca tctgttccag tacttccacc gacatcgcat
    20041 attggtgctt attgattatt ttcgctgcaa agtagccaac aatccctacg ggaatagaaa
    20101 tcgcaatacc gatgatggtt agccagccga tgtctgcgtg gagtaacccc gctgcggcga
    20161 cagggcctgg atgcggcggt accgccacat gtacagttag catgatccca gcgacaggca
    20221 ggccaaattt gagtggcgat attttggcaa ccttggcaaa accgtaaatg attggcgcaa
    20281 gaataataaa gccgacatca aagaagacgg gaataccgag gaagaacgct gccagagtca
    20341 gcgcagcgat agttcgtttg tcacctaact tgcgactgaa ataattagcc agtgactctg
    20401 caccaccaga gtgttcgatc atacgcccca gcatagcgcc cagaccaata ataatagtga
    20461 cggaaccaag cacaccgccc atcccggcga tcatcacttt acccacttcg cccgccggta
    20521 tacccgccgc aagtgcgact aacaggctga cgaggagcag agcaacgaat ggttgtacct
    20581 ttgccttgat gaccagcagc aacagcatga ttacgccagc taacgcaatg cataacaatg
    20641 taattgtgga catgggaaac cctgtctgaa agttatagtt aacctacccc atccgtagat
    20701 ggggggatgt atgggtacgt tgtaattagg gatttaacga attagcgcca ggcgtcaaac
    20761 cagccaagcc cttcttcggt gaggccacga ggtttatatt cacaaccgat ccagccctga
    20821 tatcccacct catcgaacag gcggaacagc cacggatagt tgatttctcc atcgtccggt
    20881 tcatgtcgat caggtagtcc ggcaatttgt acgtgcgcat atttcccagc gtagtcgcgg
    20941 attaaatgcg tcaggttgcc atctactttt tgcgcatgaa aagtatctag ttgaataaac
    21001 acgttatctc gcgcaacctc ttcaacaata gccagtgcct gatactggct ggagaagaga
    21061 taatgaggct taacgtcggg gctgagtgct tcaactaata ttcgcttgcc gtgtagcgca
    21121 aagcggtcgg cagcgtagcg gagattatcg ataaatactg cccggtaccg ttcagcgtct
    21181 tcgccagcgg gcacgacgcc tgccatcaca tggacttgtt cacaattgag cgccaatgca
    21241 tattccagtg ccaggtcgat gtctgcgtgt gcttcgtgct cacgtccggg aagggcggat
    21301 aatccccatt cccccgcatt aatatctccg ggagcggtat tgaacagcgc cagtgtcaga
    21361 tggttttgct ccagttgctt ttggatttgc agggtggagt agtcataggg aaacagaaat
    21421 tccacagcat cgaacccggc ttttcgcgct gcggcgaagc gttcaataaa aggcacttcg
    21481 gtgaacatca tggataaatt agctgcaaaa cgaggcattg cattaactcc ttaattccgc
    21541 aatttcacct gcggtcagat aacggatcgg gcggtcaccg agaataaaaa tcagctttgc
    21601 cgtttcctcc agctcttcca tattgttggc ggcttcttgc aggctttcac cgcaaaccac
    21661 tgggccatga tttgccagta aaaaagcctg attgtctgct gccagttccg ccagatcctg
    21721 tgcgatgcgt ttatcgcccg gtcggtaata aggcaccagc gggacatttc ccatccgcat
    21781 caccacgtat ggtgtgaacg gacgaataac gttgctgctg tccagccctt gcaggcagga
    21841 aagcgccgtc gaccatgtgc tgtgcaaatg caccaccgct ttacagcgcg gattgttgcg
    21901 atacagcgcc agatgaaaga gcacctcttt cgagggtttg tcaccactta accattcgcc
    21961 atccgcggcg actttggaaa gccgctgcgg atcgagattg cccaggcatg aacctgtcgg
    22021 tgtcgccagt aaattcccgt caggtaaaag cagcgacaga ttgccagccg aaccggttgt
    22081 atagccgcgc tgaaagaatg aactggcaat ccgcgtcatc tcctctcgca aagactgctc
    22141 tacttttgcg aaatcgctca tgataaaaac tctctttggg ctcgtgaaaa aaaggcttca
    22201 tcaccgaagt tgccagattt aagggcgagt gagacaggct tatccagtgc gtttacccac
    22261 ggcacgccgg gggaaatggt tgggccaata tgaaaccctt ttatgcccag gctctgtgtg
    22321 actacgccgg aggtctcacc gcctgcgaca ataaagcgtg tcacgccttc cgctgctaac
    22381 cgcgccgcta gttgagaaaa cagagtttct actgcctgac tggctttttg tgcaccgtat
    22441 tgctgttgaa ttgctgccaa tgcgtcagtg ctggcggtgg caaaaaccag tggagcaagt
    22501 acactttcct ggcccagaac ccactctgcc agttcgtgtg cataagcggc cagagtttcg
    22561 gttgagaggc agcgtgccac atcaacttca cgggctggtg caatttgacg gtaatgtgcc
    22621 acctggcggt tggtcatttg agagcatgaa ccggagagca ctacgccgcg cccagcgagc
    22681 ggacgccctg cttcgcgagc ctggttaccg ttttcttgcg cccactgccg ggccaggcca
    22741 atcgccagac cagaaccgcc cgttaccagt ggggcatcgc gcaaggcttc tccctgaatt
    22801 tccagatggt gttcggtcag cgcatcaagc accacgtagc gatagccctc ttgctgtaag
    22861 cgagccagct cctgacgaac ggcatctaca ccttgttcga aaacatgtgc cgaaacgacg
    22921 ccgcagcgcc ctgtggattg cgcttcaacc agacggggaa gatagctgtc ggtcatggga
    22981 tttaccgggt gatggcgcat cccggattcg gccagcagtt gattcattac gaacaaatac
    23041 ccctgataaa ccgtacgtcc gttgaccggc agggccggag agaagaccgt aaacggcgtg
    23101 tcgagagcat ccattaatgc atcggtaacc gggccgatat taccttttgc cgtactgtcg
    23161 aaagtagagc agtatttgaa atagatctgt ttgcaacctt gctgttgcaa ccagctcaga
    23221 gccgccagcg attgctgtgt ggcttcaacc accggacagg agcgcgtttt caggctgatc
    23281 accagtgcgt cgattgcttc cggcatttta cctgttggaa caccgttaat ttgtaccgtt
    23341 ggtagaccgt tttccaccag aaaactggcg atatccgtcg cgccggtaaa atcatcggcg
    23401 ataacgccaa tcttgatcat gatttcgctc ccggtagagt gatgccagag aaaatcttga
    23461 taactgcgct atcgtcttct ttcccgtaac ctgcgttact ggcgctggtg aacatattca
    23521 atgctgttga ggccaatggc agcgggaagt gcagggcttt ggctgtatcg gcaaccagac
    23581 caagatcctt aacaaaaata tcgacggctg aatgcggggt gtaatcgcca tccaccacat
    23641 gacgcatccg gttttcgaac atccaggaat ttccggcggc attggtcacg acgtcataca
    23701 tcacatccag cgggatcccc gcacgggctg caagtgccat cgcttcggct ccggcagcaa
    23761 tatgtacgcc cgctaacaac tggtgaataa tttttacggt cgaacctagt cccggttctg
    23821 cacctatgcg ataaactttt ccggcaacgg cttccagcac gggtgccagt cgttcaaagg
    23881 caatatcgct accggaggcc atgacagtca tttcaccgtt agcggctttt actgcaccac
    23941 cagaaactgg cgcatccagc atttccagat cgaatccagc cagagcggta gcaatttctt
    24001 gcgcatcagc actagcgata gtggaagaaa ccattactgc cgtaccgggt ttcagatgtt
    24061 gtgcaacgcc tgtttcacca aacagcacct gtttaacctg ggccgcattg accaccagca
    24121 ccagcagagc gtccagtttt tcggcaaacg tcgcggcgtt atcagaaacc ccgcaagcac
    24181 ctgcctcttt caacgtagcg caggcattgc tgttcaggtc tgcgccccag gtagaaagac
    24241 ctgcgcggac acatgacagt gctgctccca ttcccattga ccctaagcca acgataccga
    24301 catgaaactc agatcccgtt ttcatctgct ctccttgtta atttaagtga tattttgttt
    24361 gatattgtga atataagcgc tggaagataa cgatatggtg agctgattca cataaattaa
    24421 cattgtgtgt tattttatgt gaactaagag ttagttgcgc cgcgcacgtt tcgcaggcaa
    24481 atagcgtaga atgtcagcag gacaaaggaa ggagcaaaag ttgatacccg tagagcgtcg
    24541 ccaaatcatc cttgagatgg tagctgaaaa aggcattgtc agtattgctg aactaacgga
    24601 cagaattaat gtgtcacata tgaccattcg tcgggattta caaaaactgg agcagcaggg
    24661 agccgttgtg ctggtgtccg gaggcgtcca gtctccggga cgcgtggcgt atgaaccttc
    24721 tcatcaggta aaaactgcgc tggcaatgac gcaaaaagcg gctattggca agctggcggc
    24781 aagtcttgtt cagccgggaa gttgtatcta tctggatgcg ggaacgacca cgttagcgat
    24841 agcacagcat ctgattcaca tggagtcact gactgtggtc acaaacgatt tcgttattgc
    24901 gaactacttg ctcgacaaca gtaattgcac aattattcac actggcggtg cagtgtgtcg
    24961 ggaaaaccgt tcctgtgtcg gggaagccgc tgcgaccatg ctgcgcagcc tgatgattga
    25021 tcaggctttt atttctgcat cgtcatggag tgtgcggggg atttctacgc cagcggaaga
    25081 taaagtcacg gtcaaacggg cgattgccag tgccagccgc cagcgagttt tggtctgtga
    25141 tgcgacgaaa tatggtcagg tggcgacatg gctggcgtta ccattaagcg agtttgatca
    25201 gattatcaca gacgacggtt tgccggagag tgccagtcgc gcgctggcga agcaggatct
    25261 ctctttgctg gtagcgaaaa atgaataatg gcctgcaata acatttggtt actcatgctt
    25321 cacagaagaa gcatgagact actttatttt ataaaatgac agccgcccgc ttttcggcgt
    25381 gccggtatca atataaatct ggttagcgaa cgtctgaatg ttatcaaaca tcatatgtcc
    25441 aaatataaaa taatcagcgc cgtttatttg ttgtaactcg ccattaagcg atttctgcac
    25501 acgatcaaca ggccagagta attcgctttc tgctatttct ttaccaaact gatattcatc
    25561 acccggataa tctgcatgtg cgatggcata ttttatgttg tcattaatga tttcaataat
    25621 atgtggaagg tgatggaatt tcagcaacag atctattgcc tcttgttgct ctgaatcatt
    25681 taaatcgaaa aaccagtcac caccgctggc aagccacata tttccatcgc cagtttcgaa
    25741 tgcatccaac gccatcgctt cgtggttgcc tttaaccgac gtaaaccagg gttggtttag
    25801 caggcgcagc acgttaagac tcttcggccc acgatcaata ttatcgcctg tggaaataag
    25861 taagtcggtt tcagggtaaa aagagagttg atgtaaacgg gattgtaata actgatatcc
    25921 accatgaata tcaccaacga cccatatatg gcgatagtga tgggcattga ttttttgata
    25981 gcgtgtagat ggcatggttt taccctgtaa aataagcttt cctattatac agggtatttt
    26041 tatttgattc gtcagttgtc gttaatattc ccgatagcaa aagactatcg ggaattgtta
    26101 ttacaccaga ctcttcaagc gataaatcca ctccagcgct tgacgcggag tcagtgaatc
    26161 cgggtcgaga ttttccagtg cttcgaccgc aggcgaagtt tcttctggca ctgacagcaa
    26221 agacatctgc gtaccatcca cttgcgtcgc ggcggcgttc ggcgaaatgc tttccagctc
    26281 acgcagtttt tgccgtgcgc gcttaataac ctctttcggt acgcccgcca gagcagcaac
    26341 cgccagaccg tagcttttgc tcgccgcgcc gtcctgcacg ctgtgcataa aggcgatagt
    26401 gtcgccgtgc tccagcgcat cgagatgtac gttggcgacg ccttccattt tctccggtag
    26461 ctgggtcagc tcgaaatagt gggtggcaaa cagcgtcaat gccttaatct tattcgccag
    26521 attttctgcg cacgcccacg ccagcgacag accatcgtaa gtggacgttc cacgcccgat
    26581 ctcatccatt aacaccagac tgtattcggt ggcgttatgt aaaatattgg cggtttcagt
    26641 catctccacc ataaaggttg agcgcccgga agccaggtca tctgccgcgc ctacgcgggt
    26701 aaagatgcga tcgataggtc caatctcgac tttttgtgcc ggtacatagc tgccgatgta
    26761 ggccatcagc gcaatcagtg cggtctggcg catataggta cttttaccgc ccatgttcgg
    26821 accggtgatg atcaacatac ggcgctgcgg cgacagattc agcgggttag cgataaatgg
    26881 ctcattcagt acttgttcaa ctaccggatg gcgaccttcg gtaatgcgaa tgcccggttt
    26941 atcaatgaag gtcgggcagg tgtagttcag ggtataggcc cgttccgcca ggttcaccag
    27001 cacgtcgagt tccgccagcg cgctcgcgct ctgttgcaac gcttccagat gcggcaacag
    27061 caggtcgaac aactcttcat aaagctgttt ttccagtgcc agtgctttgc cttttgaggt
    27121 gagaacttta tcttcgtact cttttagctc tggaatgatg tagcgctcgg cgtttttcag
    27181 cgtctggcga cgcatgtagt tgatgggtgc cagatggctt tgcccacggc tgatttgaat
    27241 gtagtagccg tgcaccgcat taaagccaac tttcagcgtg tccaggccgg tacgttcacg
    27301 ctcgcggact tccagacgct ccagataatc ggtcgcgccg tcagccagcg cgcgccactc
    27361 atccagctct tcgttatagc ccgatgcgat aacaccaccg tcgcgtacca gcaccggcgg
    27421 tgtgtcgatg attgctcgct ccagcagatc gcgcagctcg gcaaactcgc ccatcttctc
    27481 acgtagcgcc tgtaccggtg cactatcgac agtttctaac tgcgcacgca gctccggcag
    27541 ttgctggaaa gcgtggcgca tacgggccag atcgcgtggg cgagcagttc gtaaagccag
    27601 acgtgccaga atacgttcca ggtcgccgac ctgacgcagt accggctgca actcggcggt
    27661 gaaatcctgc aatgcgccaa tagtttgctg gcgctcaagc aacacgcggg tatcgcgcac
    27721 tggcatatgc agccagcgtt tcagcatacg actacccatc ggcgtgacgg tgcagtcgag
    27781 cacagaagcc agcgtatttt ccgcaccgcc cgccaggttc tgagtaattt ccaggttacg
    27841 acgtgtcgcg gcatccataa tgatgctgtc ctgctcacgt tccatggtga tagaacgaat
    27901 atgcggcagg gtcgtgcgtt gggtatcttt cgcatactgc aacagacaac cggcagcaca
    27961 aagtccgcgc ggcgcgttct cgacgccaaa accgaccaga tcgcgggtgc caaattgcag
    28021 attcaactgc tggcgcgcgg tgtcgatttc aaactcccac agcgggcgac ggcgcaggcc
    28081 gcgacggcct tcaatcagcg acatctcggc gaaatcttct gcatacagca gttccgccgg
    28141 attagtgcgt tgcagctctg ccgccatcgt ttcgcggtcg gccggttcgc tcaggcgaaa
    28201 acgcccggag ctgatatcca gcgtcgcgta gccgaaacct ttgctgtcct gccagatagc
    28261 cgccagcagg ttgtcctgac gctcctgtaa cagggcttca tcgctgatgg tgcctggcgt
    28321 aacgatacgc acaactttgc gctcaaccgg acctttgctg gtcgccggat cgccaatttg
    28381 ttcgcagatg gcaacggact ctccctgatt caccagtttg gcgagatagt tttccaccgc
    28441 atggtaggga atccccgcca tcgggatcgg ctctcccgcc gaagcaccgc gtttggtcag
    28501 tgaaatatcc agcagttgcg acgcgcgttt tgcgtcgtca taaaacagtt cataaaaatc
    28561 acccatccgg taaaacagca ggatctcggg atgctgggct ttcagcctga gatactgctg
    28621 catcatgggc gtatgggcgt cgaaattttc tattgcactc atggggttat gtccggttcc
    28681 ctgatattaa atggggtgtg atggttttta ttttcgttgt aatcacataa ggcgcacatg
    28741 ataccggagt taatcatttc aggggaagaa gcgaacggcg agttcttgat gctggttccg
    28801 ggaaagatgc tgttttgcag cttttgcgtg atgcttatga agcaagtacg caaaattgta
    28861 tcggttggcg aacccttctt ctttagagtc atagtgccct ttaagattaa gatgtaatat
    28921 aaatgcatct taatgaggtg gaggcagcat gtccggcaag cgtatctctc gtgaaaaact
    28981 gacgattaaa aaaatgatcg atctttatca agcgaaatgc ccgcaggcgt cagcggagcc
    29041 ggagcattac gaggcgttgt ttgtttacgc gcaaaagcgg ctggataaat gtgtgttcgg
    29101 cgaagagaaa ccagcctgta agcagtgtcc ggtgcactgt taccagcctg taaagcgtga
    29161 agagatgaaa cagattatgc gttgggccgg gccgagaatg ctgtggcgtc atccgatctt
    29221 aaccgtgctt catctcatcg atgacaagcg tccggtgcca gaactgccgg aaaaatatcg
    29281 tccgaaaaaa cctcgtgaat agtatgccgg acaggcagat ctgtccggca atttgcagtt
    29341 aaatcaatgc cgatttatca attcccagcc gcttcatccg tgacagcagg gtcgtgcgtt
    29401 tcagccccag acgttgcgca gcgcctttag gcccggcaac cacgccgttg gtttctttca
    29461 gcacgcgcac aatcaactga tattcatctt cgccctcctg ggcgacaacc gttgcggcag
    29521 gcggcgtttc aggctccggt aaagcaatat ctggcaatga cagctgcagc acgttgccgc
    29581 gtgttagcaa tactgcgcgc tcaatgacgt tttccagttc gcgtacgtta cccggccact
    29641 ccatgttgct caaggtgcgc agcgtctcgg ctggaatgct gtcgatattg cgccccagac
    29701 gacgggcaat tttgaaggta aaggctttcg ccagcagcgg aatatcttcc ggacgctcgc
    29761 gtagtggcgg caggtgaatc gggaatacgt tcaggcggta atagagatcg ctacggaact
    29821 ctcggtcggc gaccattttt ttcagatcgc ggttagtcgc ggcaattaaa cgtacgtccg
    29881 tctgaatgat tttgttgctg ccgagacgtt caaactcctg ttcctgcaat acacgcagca
    29941 acttcggctg taactccagt ggcatatcgc ccacttcgtc gaggaacagg gagcttttat
    30001 ccgccagttc aaaacgacca atgcgctggg cgttggcacc ggtaaaagcc ccacgctcat
    30061 gaccaaacag atcgctttcc agtaatccgg caggcatcgc cgcgcagttc attttgacca
    30121 tgcggcgatt attacgccca ctgagattat ggatcgcgcg ggcaatcagc tctttacccg
    30181 tgccagtttc accgaggatc agcacggtac tgtcactttg cgccaccatt tcaacttgtt
    30241 taagcacgct gtacatggct tcgctgcggc caataatctc gccaaattca ctattaacat
    30301 tgttgagctg ctcggtcagg gcgaggtttt catcaaccag ccgttctttc agacgatgga
    30361 tttcctgata ggcgagggcg ttatcgacag cgattgccac acgttcggca atctggcgca
    30421 gtaaattcag attggtagtg gtaaacactt tctcttcgca ttgcgccagt ttcagcacgc
    30481 ccagcatggt gtcgccagac atcagcggta acaggcacaa ggtttgaatc tggttgcccc
    30541 aggtgtcgaa caacatgcgt tcataggggg ctaaatcgtc ccgctcgtgg agattgatca
    30601 gcagcatctc tttacttttg aacacgcgtt cggtgagggt tccggcttca tcgacttcgc
    30661 tctgttcgtg ggcgggatgt tgtttatcaa gatagtgagt ggagtagatg ttgagtttgt
    30721 ttttacggtg gctgcgtaag acaatgctga tatcgtcaat gtcgaaatag taatggattt
    30781 ctttggcgac ttcgctgacc agttcgtcca tatccaggcg ggaaagcacc gcgttggtaa
    30841 tggcaaccag gatgcggaag ttatcgcgct cccggcataa caactcatag tcgacattgt
    30901 tgacgacgcg gctctggatt tgttcggtga cgacagaaac gatctgcgta aatgtttgca
    30961 gacgattgaa ctctttttcg ctccagggac gatcgtcata acgaataaat tcacagccac
    31021 caaaaatatg cccttccgcc gccagtggca tcaggcaata gtgaccaaat ttggggtata
    31081 gcccacctgc ggccagctgc ggccaggttt cacaaaattc ttcgtaactg caatgcagcg
    31141 tatcagggcg cgacaaaatg ctgcgtaccg gaccgtgtgc cagaacagtt tcgtcttcat
    31201 atttaatggg ggtgtctttt tcacgcgacg cgtaataaga cgcacgttga gtctgcgctt
    31261 gccacaacac aatagccgcg ttgtcggcga gcgcagaacg ctttaccagt tgcgaaagag
    31321 cctcacacag cgaggccaga tcgggctgct gcaatagtgt ccgagtgatg tcgaacaacc
    31381 cttgctgtcc gagatcactc atcggtgtat atgacatttt gttcttccag gaaagcaccg
    31441 caaagcggtg caaaaggtca ttgactaact ataggagatt tagaatttta ttagcatata
    31501 cgcggaagcg gttcggcgtg tggtaaatcg agggttcgtt tcacgccata cagaccggca
    31561 agacgaacac ctttacgttc caccacttca ccaatcagcg ccgcgtcttt ccccagtgga
    31621 tgggaatgta acgctgccag cacttgctct gccgcgttgc gttcaacggc gatcaccagt
    31681 ttgccttcgt tggcaaagtt aagggcgtcc agtcccagca gttcgcaaac gccgcgcacg
    31741 gcaggtttaa ccggcagtga cgcttctgaa atttcaatac cacaaccgca ggctgccgcg
    31801 aactcatgaa ctaccgcgtt cacaccacca cgggtggcat cacgcagcgc tttcacgccg
    31861 ggaatgtcac gcagcgtctg aataagcggc gtcagcaccg cgcagtcgct gaccagttcg
    31921 ccatccagcc ccagctgctc acgcaggtta aggatagtcg ccccgtggtc gccgagtgta
    31981 ccgctaacca gcaatacatc gcctgcggtt agcgtctgtg cgccccagtg aatattcgcc
    32041 ggaattgcgc ccatgccagc ggtgttgata aacagtttat ctgccgcgcc gcgctgcacc
    32101 actttagtat cgccagtaac gatggcaatg cctgccgtgc gggcggtttc tgccatgctg
    32161 gtcactacgg ctttcagtgt ctccatcggc aatccttctt cgaggataaa gccacaggag
    32221 agatagcgcg gaatagcgcc actgaccgca acgtcattcg cggtgccgca aatcgccagc
    32281 ttgccgatat taccgccagg aaagaacagc gggtcaatga cgtaactgtc ggtggagaac
    32341 gccagacggt cgccttctgc taccagctgc gccagatcaa gacgtgcctg atcttcctgc
    32401 tctgccagcc acgggttggc aaaggcttcc ataaacaggc tgttgattaa ttgctgcatc
    32461 gcctggccgc cgctgccgtg ggcgagttgg atattattca cgcttcactc tcctgctgac
    32521 gatactgata ccacgcggcg cacgctcctt cggaggagac catcagtgca ccaaatgcgg
    32581 tttgaggatt acaggtgtta ccaaacagcg ggcattgatg cggcttacat ttgcccgtta
    32641 atacctcacc acaacgcgcg cgcgggtcat cgcagacctg ctgcggtgcc gggcggaaat
    32701 gtgcttcggc atcgaatcgt tgataatccg gcgtcaggtg cacgccagaa gattcaatca
    32761 cgcctaagcc gcgccattcg ctgtcgccgt tgacacagaa cacatcggca atcgcctgtt
    32821 gcgccagcag gttaccggca tccggtacca ctcgacgata ctgattctct accttgctgt
    32881 gggccgctat tttctgctcc accagcatga ccacgccttg cagtagatca aggggttcga
    32941 aaccagcgac caccagcgga cgatgaaaat cgctggcgat aaaattatag gcatcagtgc
    33001 cgataaccat actaacgtgg cccggcgcga ggaacgcgtc gataccgtta tccggctgtt
    33061 ccagcaaact gcgcagcgtc gggataagcg taatgtgctg gcagaagaag taaaaattct
    33121 gcacatcacg cgctttcgcc tgttgcagag tgatggcggt ggtcggcatg gtggtttcaa
    33181 aacctaagcc gaagaacacc actttgcggg ttggattctc ctgcgccagt ttcaacgcat
    33241 ccatcggcga gtaaacgatg cgcacatcgg caccgcgtgc ttttgcctgc aacagcgatc
    33301 cctgtttccc tggtacgcgc atggcgtcgc caaaggtaca gaagatgact tccggatggc
    33361 tggcaatctc cacgcaggtg tcgattctac ccatcggcag tacgcacacc gggcaacccg
    33421 gaccgtggat aaactcaacg ttttcaggca gtaactggtc gaggccgaat ttaaaaatgg
    33481 cgtgggtatg accaccgcac acttccataa tccgcagagg gcgttcggcg gtgtaagaga
    33541 gatgtgaagc acgttcgcgc agatgctcaa ttaactgcat cacctgttcc ggcgcgcgat
    33601 attcatcaac aaaacgcatt atttttcctc gccatacaac agcgcgccga catccggctc
    33661 aacgtcaaac atgttttgta aggcgtcgag agtgtcgcgt gcttcggctt cattaattac
    33721 gctcatggca aagccaacgt gtaccagtac ccactggccc acgcgcggct gaccgttttc
    33781 atcgcagctg ccgactaacg ttaaatcgac atcgcgctga atgccgcaga cgtcgacttt
    33841 tgcctggttg ccgtcaatgg tgcggatctg gccgggaacg cctatgcaca tcgctgtgtc
    33901 tccagccagt tcagccactg gtccatccct tcgccgctgg tggcggaaat aaggatgatt
    33961 tcaatttctg gattgacttc gcgggcgcag gcgatgcact tctcaacgtc aaagttgaga
    34021 tacggcaaca ggtcaacttt gttgagcagc atcagcgagg cggcggcaaa catatgcgga
    34081 tatttcagcg gtttgtcttc accttcggta acggaaagca ccgccacttt gtgtttttca
    34141 ccgagatcga agctggccgg gcatacgagg ttgccaacgt tttcgataaa cagaataccg
    34201 ttatcgtcca gtggcagacg cggtgcggcg tcggcaatca tctgtgcgtc aagatggcag
    34261 cctttacctg tgttcacctg aatcgctggt gtgccggtag cgcgaatgcg tgcggcatcg
    34321 ttcacggttt gctggtcacc ttcaataacc gcgcacggaa cgctgtcttt caggcgcatt
    34381 agggtttccg tcagcagggt ggttttaccg gaacctgggc tggaaaccag gttgagcacc
    34441 agttgcttgc gggcagcaaa gcgcgcgcgg ttgcgttcag ccagacggtt gtttttgtcc
    34501 agcacgtcaa tttcgacttc cagcatccga cgctggctca tgcccggtgc gtgagtgccc
    34561 gcttcgccat gaccgtaatg caggtcgcct tcttcagtct ggctgggggt aaattcaggc
    34621 gctttaatgc cggtgatttt catcttcggg cgtgccgccg gggcaaatgg cgcgctacga
    34681 aacgcggaat gagggttatg ttcatcaccc tcgatataca ggttgccttt accgcaaccg
    34741 catgttgtac acatcgctca ctcctggtct atttctatcc gccgaatctg taaaccgtcg
    34801 tctgccacaa tctgcagcat gtcaccatga cactgtggac agcggcggac gcgctgggtt
    34861 agtagcgtca catactgttg gcatgtttca caccagcatt cggcctcttg ttcttcgagg
    34921 tgcagtttac aaccttccgc cacgctgccg cggcaaacca gatcaaaaca aaaggcaaga
    34981 gagctggttt cgacacaaga aaatgcgcca attttgagcc agaccccagt tacgcgtttt
    35041 gcgccgtgtt ttgcggcctg ctgttcgatc aattccagtg cccgttggca gagggttatt
    35101 tcgtgcatat cgcctcccat taactattgc cagctacaag caataattgt gccagtgttg
    35161 attatccctg cggtgaataa tgtcgatgat gtcgaaatga cacgtcgaca cggcgacgaa
    35221 attcatcttt agcttaaaaa tcgttttaat aacaataaat taaaagttgg cacagaaaat
    35281 gcttaaagct ggcatctctg ttaaacgggt aacctgacaa tgactatttg ggaaataagc
    35341 gagaaagccg attacatcgc acagcggcat cgtcgcctac aggaccagtg gcacatctac
    35401 tgcaattcgc tggttcaggg gatcacgtta tcgaaagcgc gcctgcatca cgccatgagc
    35461 tgcgcgccgg acaaagaact ctgtttcgtc ctttttgaac attttcgtat ttacgtcacc
    35521 ctggcggatg gctttaacag ccacaccatc gagtattacg tcgaaacaaa agagggcgaa
    35581 gacaaacagc ggattgcgca ggcgcaactg agcattgacg gcatgattga tggcaaggtc
    35641 aacatccgcg atcgcgaaca ggttctggaa cactatctcg aaaaaatcgc tggcgtttac
    35701 gacagcttat acaccgctat tgaaaacaat gtgccggtga atttaagcca actggtaaag
    35761 ggacaaagcc cggcagcatg agctgaggct ttgcccgttt tgcaggcgtt acgcctgttt
    35821 ggggatgggc gtgtcgatga gtgtcgaaaa tgacatttca tcggcatgtt ttcgtcaaaa
    35881 atgacaatca cctgaggaat gcctggtgaa tcgttttgta attgctgact ccacgctctg
    35941 tatcggctgc cacacttgtg aggccgcctg ttcagagacg catcgccagc acggcctgca
    36001 atcaatgccg cgcctgagag tgatgctgaa tgaaaaagaa tctgcgccgc agctctgtca
    36061 ccactgtgaa gatgcaccct gcgcggtggt ctgcccggtt aacgccatca cccgcgttga
    36121 tggggccgtg cagttgaatg aaagcctgtg cgtaagctgc aagctgtgcg gcatcgcctg
    36181 cccgtttggc gcaattgaat tttccggcag ccgtccgctg gatattccgg caaacgccaa
    36241 taccccgaaa gcgccaccgg caccgcctgc tccggcgcgt gtcagcacat tgcttgactg
    36301 ggtgccaggt attcgcgcga tcgccgtcaa atgtgacctt tgtagctttg atgaacaagg
    36361 tccggcctgc gtgcggatgt gcccgactaa agccctgcat ctggtggata acactgatat
    36421 cgcccgcgtc agcaaacgta agcgtgagct gacttttaac acggactttg gcgatctcac
    36481 cttgtttcag caggctcaaa gtggagaggc taaatgagcg caatttccct gatcaatagc
    36541 ggcgtggcgt ggtttgtcgc cgccgctgtt ctggcatttc tcttttcttt tcaaaaagcg
    36601 ttaagtggct ggatagctgg aattggcggc gcggttggta gtctgtatac ggcagccgcg
    36661 ggcttcactg tactgactgg cgcggttggc gtgagcggtg cgctgtcgct ggtaagctac
    36721 gatgtgcaaa tctctccgct taacgcgatt tggctgatta cgctcggtct gtgcggtctg
    36781 tttgtcagcc tctacaacat tgactggcat cgccacgcgc aggtgaagtg caacggcttg
    36841 cagatcaata tgttgatggc tgccgccgtc tgcgccgtca tcgccagcaa cctcggcatg
    36901 ttcgtggtaa tggccgaagt catggccttg tgtgcggtgt tcctcaccag caacagcaaa
    36961 gagggcaaac tgtggtttgc gctggggcgt cctggcactc tgctgctggt gattgcttgc
    37021 tggctgctgt ggcagcgtta tggcacgctg gatctgcgcc tactggatat gcgtatgcaa
    37081 cagctgccgc tcggttccga tatctggctg ctcggtgtga ttggctttgg cctgctggcc
    37141 gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc tgcgccagct
    37201 gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt aaccctgtca
    37261 ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct cggcatgatc
    37321 accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg cctgctggct
    37381 taccacaccc tggaaaatat cggcattatc ctgctggggc tgggcgctgg cgtaacgggt
    37441 atcgcgctcg aacaaccggc gctgattgct cttggcctgg tcggtggtct gtaccatctg
    37501 cttaaccata gcctgttcaa aagcgtactg ttcctcgggg cggggagcgt ctggttccgt
    37561 accggtcatc gcgatatcga aaaactcggt ggtattggca agaaaatgcc ggttatctcc
    37621 atcgccatgt tagtcgggtt gatggcaatg gctgcgctgc cgccactgaa cgggtttgcc
    37681 ggggaatggg gtatctatca atccttcttc aaactgagca atagcggcgc gtttgttgcc
    37741 cgtctgctgg ggccgctgct cgctgtgggt ctggcaatta ccggtgcgct ggcggtgatg
    37801 tgtatggcga aagtctatgg cgtcacgttc ctcggcgcgc cgcgtaccaa agaagccgaa
    37861 aacgccacct gtgcgccgct cctgatgagc gtaagcgtag tggcactggc gatttgctgc
    37921 gtaattggcg gtgttgctgc gccgtggctg ctgccgatgc tctctgctgc tgtacctctg
    37981 cctctggagc ctgctaacac caccgtttct caaccaatga tcacattgct gctgattgcc
    38041 tgcccgctgc tgccattcat cattatggcg atttgcaaag gcgaccgcct gccgtcacgt
    38101 tcccgcggtg cggcctgggt gtgcggctac gaccatgaaa aatcaatggt gattaccgct
    38161 cacggttttg ccatgccggt taaacaggtg tttgcgccag tgctgaaact gcgcaaatgg
    38221 ctgaatccgg tgtcgctggt gccgggctgg cagtgcgaag gtagtgcgtt gctgttccgc
    38281 cggatggcgc tggttgaact ggcggtactg gtggtgatta ttgtttcacg aggagcctga
    38341 gaatgagtgt tttatatccg ttaattcagg cgctggtgtt atttgccgtt gcgccgctgc
    38401 tctccggtat tacccgcgtg gcgcgcgccc gtctgcataa ccgtcgtggg ccgggtgtgt
    38461 tgcaggagta tcgtgacatt ttcaaactgc tgggccgcca gagcgttggc ccggatgcct
    38521 ccggctgggt gttccgcctg acgccgtatg tgatggtggg cgtcatgctg actatcgcta
    38581 ctgcgctgcc ggtggtgacc gtcggttctc cgctgccgca actgggtgat ttaatcacct
    38641 tactgtatct ctttgccatc gcgcgtttct tctttgccat ttctggtctg gataccggta
    38701 gcccgtttac cgctatcggc gcgagccgtg aagcgatgct tggcgtgctg gtcgaaccga
    38761 tgctgctgct tggtctgtgg gttgccgcac aggttgccgg ttccaccaac atcagcaaca
    38821 tcaccgacac cgtttatcac tggccgctga gccagagcat cccgctggtg ctggcgcttt
    38881 gtgcctgtgc gttcgccacc tttatcgaaa tgggcaaact gccgttcgac ctggcggaag
    38941 ccgagcagga gttgcaggaa ggtccgctct ctgagtacag cggcagcggc tttggcgtca
    39001 tgaaatgggg tatcagcctg aaacagctgg tggtgttgca gatgttcgtc ggggtgttta
    39061 ttccgtgggg acaaatggaa accttcaccg ccggtggact gctgctggcg ctggtgattg
    39121 ccatcgtaaa actggtggtc ggcgtcctgg ttatcgcgct gttcgaaaac agcatggccc
    39181 gtctgcgtct tgatattagt ccgcgcatta cctgggctgg gtttggcttt gcatttttag
    39241 cgttcgtctc cttgctggcg gcgtgattaa agagagtttg agcatgtctg aagaaaaatt
    39301 aggtcaacat tatctcgccg cgctgaatga ggcatttccg ggcgtcgtgc tggaccacgc
    39361 ctggcagacc aaagatcagc tgactgtcac cgtaaaggtg aactacctgc cggaagtggt
    39421 ggagtttctt tactacaaac agggtggctg gctgtcggtg ctgtttggta acgacgaacg
    39481 caaactgaat ggtcattacg ccgtttacta cgtgctgtcg atggagaagg gcactaagtg
    39541 ttggattacg gttcgcgtcg aagttgacgc caacaaaccg gaatatccgt ccgtgacacc
    39601 gcgcgttccg gcggcggtgt ggggcgagcg cgaagtgcgc gatatgtacg gtttgattcc
    39661 ggttggtctg ccggatgaac gccgtctggt gctgccggat gactggccgg atgaacttta
    39721 tccgctgcgt aaagacagca tggattatcg tcagcgtccg gcgccgacca ccgatgctga
    39781 aacctacgag ttcatcaacg aactgggcga caagaaaaac aacgtcgtgc cgattggtcc
    39841 gctgcacgtc acttccgacg aaccgggcca cttccgtctg ttcgtcgatg gcgaaaacat
    39901 tatcgacgcc gactaccgcc tgttctacgt tcatcgcggt atggaaaaac tggcggaaac
    39961 ccgcatgggt tataacgaag tgaccttcct ctctgaccga gtttgtggga tctgcggctt
    40021 tgcccacagc accgcctaca ccacgtcagt ggaaaacgcg atgggtattc aggtgccaga
    40081 acgtgcgcag atgatccgcg ccattctgct ggaggtggaa cgcctgcact cgcatctgct
    40141 caacctcggt ctcgcctgcc actttaccgg ctttgactcc ggctttatgc agttcttccg
    40201 cgtgcgtgaa acctccatga aaatggcaga gatcctcacc ggtgcgcgta aaacctacgg
    40261 cctgaacctg atcggcggga ttcgtcgcga tctgttgaaa gatgacatga tccagacccg
    40321 ccagctggcg caacagatgc gtcgtgaagt gcaggagctg gtggatgtgc tgctgagtac
    40381 gccgaacatg gaacagcgca ccgtcggcat tggtcgtctg gacccggaaa tcgcccgcga
    40441 tttcagtaac gttggcccga tggttcgcgc cagcggtcac gcccgcgata ctcgcgccga
    40501 tcacccgttt gtcggttatg gcctgctgcc aatggaagtc cacagcgagc agggctgcga
    40561 cgttatttcc cgtctgaaag tgcgtatcaa cgaagtttat accgcgctga acatgatcga
    40621 ctacggtctg gataacctgc cgggcggtcc gctgatggtg gaaggcttta cctacattcc
    40681 gcaccgtttc gcgctgggct ttgccgaagc gccgcgcggc gacgatatcc actggagcat
    40741 gaccggcgac aaccagaagc tgtaccgctg gcgctgccgt gccgcgacct acgcgaactg
    40801 gccgaccctg cgctacatgc tgcgcggtaa caccgtttcc gatgcgccgc tgattatcgg
    40861 tagcctggac ccttgctact cctgtaccga ccgcatgacc gtggtcgatg tgcgtaagaa
    40921 aaagagcaaa gtggtgccgt acaaagaact cgagcgttac agcattgagc gtaaaaactc
    40981 gccgctgaaa taaggaatcg ccatgtttac ctttatcaaa aaagtcatca aaaccggcac
    41041 ggcgacctcg tcttatccgc tggagccgat tgcggttgat aaaaacttcc gtggtaagcc
    41101 ggagcagaac ccgcagcagt gcatcggctg tgcggcctgc gtcaatgcct gcccgtcaaa
    41161 cgccttaacg gttgaaactg acctcgccac aggagagctt gcgtgggagt ttaatctcgg
    41221 gcgctgcatc ttctgtggac gctgcgaaga agtttgcccg acggcggcga tcaaactgtc
    41281 gcaagagtac gaactggcag tgtggaagaa agaagacttc ctgcaacagt cccgcttcgc
    41341 gctgtgcaac tgccgcgtct gcaatcgtcc tttcgccgtc cagaaagaga tcgactacgc
    41401 cattgcgctg cttaagcaca acggcgacag ccgcgcggaa aaccaccgcg aaagctttga
    41461 gacttgcccg gaatgtaagc gccagaaatg cctggtgccg tccgaccgta ttgaactgac
    41521 tcgccatatg aaagaggcca tctgatgagc aatttattag gcccccgtga cgccaacggc
    41581 attccggtcc cgatgacggt ggatgaatcc atcgccagca tgaaggcgtc gttactgaaa
    41641 aaaatcaaac gttctgccta cgtttatcgc gtggactgcg gcggctgcaa cggctgcgaa
    41701 atcgaaattt tcgccacgct ttcgccgctg tttgatgcag aacgcttcgg cattaaagtc
    41761 gttccttcac cgcgtcatgc ggatatttta ctgtttaccg gcgcggtcac ccgtgcaatg
    41821 cgatcccctg cgctgcgtgc gtggcagtcc gcgccggacc cgaaaatttg tatctcctac
    41881 ggtgcctgcg gtaacagcgg tggtattttc cacgatctct actgcgtgtg gggcggtacg
    41941 gataaaatcg tcccggtaga tgtctacatt cctggttgcc cgccaacgcc tgccgccacg
    42001 ctgtacggct ttgcaatggc gctcggcctg ctggagcaga aaattcacgc ccgtggcccg
    42061 ggtgagcagg atgaacaaca ggcggaaatc ctgcatggcg atatggtgca gccgctgcgt
    42121 gtgaaagtgg atcgcgaagc acgccgcctg gcgggttatc gttacggtcg tcagattgcc
    42181 gatgacttcc tgacacagtt agggcagggc gaagaacagg ttgcacgctg gctggaagcg
    42241 gaaaacgatc cgcgtctgaa cgagattgtc agccatctga atcatgttgt tgaagaggcg
    42301 cgtatccgat gagtgaaaag gtggtgttca gtcaactgag ccgtaaattt attgatgaga
    42361 acgatgccac gcccgccgag gcgcagcagg tggtctatta cagcctggcg attggtcacc
    42421 accttggggt tatcgattgc ctggaagcgg cgctaacctg cccatgggat gaatatctgg
    42481 catggattgc cactctggag gcgggcagcg aagcccgccg caaaatggaa ggcgtaccga
    42541 aatatggcga gatcgtcatc gacattaacc atgtgccgat gctggccaac gcattcgata
    42601 aagcccgggc agcgcaaact tcgcagcaga aggaatggag tacaatgctg ttaagtatgc
    42661 tgcatgatat tcatcaggaa aacgccatct atttgatggt gaggagactg cgtgactgac
    42721 gttttactct gtgttggcaa tagcatgatg ggcgatgatg gcgctggccc gctgctggcg
    42781 gaaaagtgcg ccgccgcgcc gaaaggtaac tgggtggtga ttgatggcgg tagtgcaccg
    42841 gaaaacgaca tcgtcgccat ccgtgaactg cgccctacgc ggttactgat tgttgacgct
    42901 actgatatgg ggctaaaccc cggcgaaatc cgcatcatcg acccggatga tatcgccgag
    42961 atgtttatga tgactaccca taacatgccg ctgaactatc ttatcgacca gttgaaagaa
    43021 gacattggcg aagtgatttt cctcggcatc cagccggata tcgtcggctt ttactacccg
    43081 atgactcagc cgattaaaga tgcggtagaa accgtttatc aacgactgga aggctgggaa
    43141 gggaatggcg gcttcgcgca gttagcggtg gaagaagaat aattctccat cagggaattc
    43201 agcgaaatga atatcagaac agggatgtgc gcttttttat tgagcttagt gctgcctgca
    43261 caggccacca gctttaccga atatctgcca atgagtgaca gcgaatacgc gcaaaagcgg
    43321 gcgctgaaac ctctcctgac catgccttat gatgccgagc aaacctggca ttttagaaaa
    43381 gtgggcgttg ctggcgtgac gcttgaaaag atgccaaatg atgatagtga atggcaattg
    43441 aacggtaaag atcgcgccgg taaatcctgg tctgtgcctg tgggtatgct ggcaaacata
    43501 gcgggcaaag ggcaactcta tcgcgccgat cttgatcgta acggcattca ggatctggtt
    43561 atctggttac caagtaccgg taatggtctg gctccttatg cacatttaat tcttatgacg
    43621 ttcacgcgcg aaggccgtcc ctgtgtcttt gaacccaggg gattctacac cgcaagcaaa
    43681 actggcgtag atgatttgct ggatttgcaa ggcaatggtc acacgcaatt gctggatatg
    43741 cagtttgatt ccggttactg gataaccagt ctgtatcagg taaaagacgc caaatggcag
    43801 cgtgttcacg gctggtttgg caagttaagc tatcccgcgc tcacgcgttt tacttatacc
    43861 cccaaccgta aactcgtcct caaacccatc gccgggcgcg acccgcaaac ggaagatctg
    43921 gcgcagacgc agcgttgctt gataaagggg gatgttcttg atggggtgaa ccagaactga
    43981 ctttttgcag agtggctatg aggattttgg gggtaattat atcgcgctca agtgtttaca
    44041 tattgtgaac ttggatttga tgagctatag tttccgaaca cataaggata gaactcgcag
    44101 atgtggctgt ttacttacta taaggaagtt aaaacttaaa aatgcacatc atttctaaag
    44161 cgccatttga agagtctgcg cgtaaatatc ctaacgacgc tctggctctt caggcattgt
    44221 atcgggttat taaggaaacg gatttttcta cgccagaaga gatgcgaacg gcgtttccca
    44281 acctcgacaa ttttaaatat cgcaacaaat ggtacgttct ggatgtaggt gggaacaacc
    44341 taagggtgat tgcctacata aactttgtta ataagcggtt ctttgtgaag cacataacta
    44401 atcacgccga gtatgacaaa ctgacacgtt actacagaga gaacaaagaa tgactgctaa
    44461 tgctgcacgc gctgttaaag caacaagaga actggtcaat gcagtcccct ttctgggagg
    44521 tagtgactca gaagatgatt atcgtgaagc actggaactg gtcgaatatt tgattgaaga
    44581 agatgacacc aatccactta tcgactttct ggcgagccgc attgcagaat atgaaaacaa
    44641 caaccaagag tttgcagagt tcgacaaagc cgttgcggca atgcctgttg gcgtggcgtt
    44701 acttcgcacg ctgattgatc aacataatct gacctatgcg gatttgaaga acgaaatcgg
    44761 ctctaaatcc ctggtcagtc agattttgtc tggacaacga tccctgacga tatcccacat
    44821 taaggcactt tcagcccgat ttggcgttaa gccggaatgg tttctttgat tttgactgat
    44881 gccaaacccc agattgcccg atgcgttgcg cttatcgggc cttcctggtt cgtgcgacat
    44941 gtaggccgga taaggcgttc acgccgcatc cggcactgtt gcttactcta aatcttcacc
    45001 attactggca atcacttttt tataccacca gaacgatttc ttacgtgtgc gcgtcagcgt
    45061 gccgttgcct gcgtcgtcac ggtctacgta gacaaaaccg tagcgtttgc tcatttcacc
    45121 cgtagaggcg gaaactaaat caatacagcc ccatgtggtg tagcccatca gcggaatgcc
    45181 atctgcaatc gcttcgccca ttgcgcggat atgttcgcgt aaatagctga tgcgatagtc
    45241 atcgttaatc tcgccattgg cagcaagttc atcttttgcg ccgaggccgt tttccaccag
    45301 aaacagcggc ttctgataac gatcgtacat catattcatg gtaatacgca gaccgagtgg
    45361 atcaattccc cagccccagt cgctcacctg tagatacgga ttacgcagtg atttcaccac
    45421 gttcgccgca ctgctgttgt tggcgttcat ctccgccgag gcgcagcgcg aggcgtaata
    45481 gctgaaagag acaaaatcga cggtgttttt caggatttca tcatcgcccg gtgctttgtc
    45541 gatggttacc cctttttcgc ggaatacgcg ggcagagtaa gccggatacg tgccccgcgc
    45601 ctgcacatcg ataaaaaaca ggttttcccg gtctttctcc agcgccgccc agacatcttc
    45661 cggcttgcaa ctgtaagggt agaagttacc gcccgccagc atacagccca cctgattttg
    45721 cgggttaacc tcattggcga ttttggtggc tagcgcactg gcaagcagct ggtgatgcgc
    45781 ggcctgatat ttcacctgat cctgattttc accttcttca aacaccagac ccgcgccgga
    45841 gaacgggcta tgcaacataa tgttgatttc attgaaggta agccagtatt tcaccagacc
    45901 atcaaatgct tcaaagcagg tccgggcgta gcggctgaaa aactccacca gcttgcggtt
    45961 acgccaggag ccatattcgg tgaccagatg catcggccca tcgaagtggc acaacgtgac
    46021 cagcggttcg ataccgtact ttttacactc ttcaaagaca gaacgataaa aagcaatgcc
    46081 ctgctgattg ggcgtgattt catcgccctg cggaaagaga cggctccagg caattgaggt
    46141 acggaaaacc ttgaatccca tctctgccat cagggcgata tcttctttat aacgatgata
    46201 aaaatccgtc gcctcatggc tgggataaaa ctcgtcatcg cgcaactgaa aacgtttttc
    46261 cagccccagt ttcaccgcca ttcgatgctc gccgtgtggg atcatatcga cagtggtcag
    46321 ccctttgcca ccttcacgga acgcaccttc agactggttg gcggcaagcg cgccgcccca
    46381 taaaaaacct tctggaaata ctgacatttt catcctcaat taagacttac ttctttggcg
    46441 acggtcggtt gtacgctctg atgctttcgc gcctcggcag ccgcttcctc aacaggaata
    46501 tcctcaaagc cgagcaacag tgtgaggata aacgacagca ccaccgccag cgccatgacc
    46561 gcgaacaccc agacgatgct cattggattc gccggatcga agaattgcac gctggtaaat
    46621 aaccctggcg ctgccattga gtggctggca agccccgcca taccggcaac cgcgccgcaa
    46681 ataaaaccgc tgataagact ggcgataagc ggacgtttca ggcggatcgc cacgccgtat
    46741 aacgccggtt cggaaatccc cgccataatg gctgatgccg ccgcagccag cgccgtctgg
    46801 cgcagttccg ggtttttcgt tttccacgcc accgccagtg atgaaccgcc cagcgacagg
    46861 ttagcgccga tctctgacgg catgaccatc ccttctttgc cggtttcggc aatggtctga
    46921 atgatggttg gcgtaaagac gcggtgcatc ccggtcatta ccagcagagg ccacagcgcg
    46981 cccataatgg cgactgaaag ccagcccaga taaccatgaa tggtgtaaac cagcgccgaa
    47041 atggcgctac cgatccagat accaatcggg ccaatcagca ggattgccag cggtgcggca
    47101 atcaacacaa tcagcattgg cttgaggaag tttttggtca ccgccggtgt aatgcgatcc
    47161 acccagcgtt cgatatatga caggcaccag gtcatcacca gcgccgggat cactgtgtag
    47221 gtgtatttca ccgcggtgac cggaatcagg gcaaattcga catgttcacc ctgggccgct
    47281 ttcgccatca gttcaataaa actcggatgt accagcacac ccgcgatggc aatcgccagc
    47341 gacatattgg ttttaaattt gatggcggca gatgccgcga ccatcagcgg cagaaagaag
    47401 aaagcaccgt caccaatcac attcagaatg gttaaggtcg gcgatccttt tgtcagcacg
    47461 ccgctcatct cgaggatcat cgccagcagt ttgaccatcg atccgccgat aatcgccggg
    47521 atcagcggtg acatggtgcc gatcagcgca tcgaggatcc ccgcaccaat gcgacgcagc
    47581 gtgagtttgg gtttacccac gggctgtgcg ggctgcatat ctcccggcag caggctgacg
    47641 acttcctgaa aggcttgtga aacggtattg ccgataatca cctgacactg gttgtcacta
    47701 cgtaccacgc cgagcacgcc ggggatggtt tttaacgtcg ggctgtcgat aagctggtca
    47761 tctttgataa caaagcgcaa acgcgtcata cagtgcgtga ccgccgagat gttatcaacg
    47821 ccgcccagtg ccgctatcac cgagcgtgcc agcgccgcat aatttttggc catcggattt
    47881 tatcctgtta tcagtagggt agatgtagtt gttatcagtc gacgtaacgg aaaaatgccg
    47941 atgctgaaac ggaattagga aaccggtttc acaaaatcat gaatagttgt cactaaataa
    48001 gcaagagagt taattgttca ttttttatat ttgtgaaacc ggtcacatga atatttttcc
    48061 cgccgattgc gcaaggacaa tcggcaaacg gtgcagtaca ctgcgttgac tgcgtgtaaa
    48121 gggatgaaaa tgatgacgac gatgctggaa gtggcgaagc gcgccggggt ttcaaaagcg
    48181 accgtttccc gcgtgctttc aggtaatggc tacgtcagcc aggagactaa agatcgcgtg
    48241 tttcaggcgg tagaagagag cggttaccgc ccaaacttgc tggcgcgcaa tctgtcggcg
    48301 aagagtactc agacgctggg gctggtagtg accaacacgc tttaccacgg catttatttt
    48361 agtgaattac tctttcatgc cgcgcgaatg gcggaagaga aagggcggca gttgctattg
    48421 gcagatggta aacacagcgc cgaagaagag cgccaggcga ttcagtatct gctggatttg
    48481 cgctgcgacg ccattatgat ttacccgcgt tttttaagcg tggatgagat cgatgacatc
    48541 attgacgcgc acagtcagcc gataatggtg cttaatcgcc gcctgcgcaa aaacagcagc
    48601 catagcgtct ggtgcgatca taaacagacc agctttaatg ccgtggcaga gttgataaac
    48661 gccggacatc aggagattgc tttccttacc ggctcgatgg attcccccac cagcattgaa
    48721 cgtcttgctg ggtataaaga cgcgctggcg cagcatggta ttgcgctcaa tgaaaaactt
    48781 atcgctaacg gtaaatggac gcccgccagc ggggccgaag gggtagaaac gttgctcgaa
    48841 cgtggggcta aatttagcgc gttagttgcc agtaacgacg atatggcgat aggtgcgatg
    48901 aaagcgttgc acgagcgcgg cgtagcggtg ccagagcagg tgtcagttat cggattcgat
    48961 gatatcgcaa ttgcccccta tatcgttccg gcgctctcca gcgtaaaaat tccggtgact
    49021 gagatgattc aggaaattat tggacggctg atttttatgc tcgatggcgg ggatttctca
    49081 ccgccgaaaa ccttcagcgg aaaactgatc caccgcgact cactcattgc tctttcgcga
    49141 taaacattgt catcacatgt catcgtcact agtgtcgatg acatcttttt tcctgcatta
    49201 cagaaattaa tttccttaaa taacagtgaa ttaaaaactg gcatgatttg tgaatgtatc
    49261 ggcgcattaa ctgtcattgc tggagaattt gatgaaccgt ttcatcattg ctgacgcgag
    49321 taaatgtatt ggttgccgta cctgtgaagt ggcgtgcgtg gtttctcatc aggaaaatca
    49381 ggactgtgca tcgctgaccc cggaaacatt tttaccgcgt atccatgtca ttaaaggtgt
    49441 gaacatttcc acggcgacag tttgccgtca gtgtgaagat gcaccgtgcg ctaacgtctg
    49501 cccgaatggt gctatcagcc gtgataaagg gtttgttcat gtgatgcagg aacgttgcat
    49561 tggttgcaaa acctgcgttg tggcttgccc gtatggcgcg atggaagtgg tggtacgtcc
    49621 ggtgattcgc aacagcggcg cggggctgaa tgtacgggct gacaaagccg aagccaataa
    49681 atgtgacctg tgcaaccatc gtgaagacgg cccggcgtgt atggcggctt gcccgactca
    49741 tgcgctgatt tgtgtcgatc gtaataaact tgaacaactg agtgcagaaa aacgccgccg
    49801 tacggcgctg atgttctaaa aatcttacag aactgttctg gcatcaaagg aattcccgga
    49861 aatattcgta gaatacggag cgggcggtat cgccgacctg tgactgccgg atgcgacgct
    49921 gtcgtgtccg gctgggcggg ttacgcaatt catgatggcg ggaataactc aatggcaaaa
    49981 aacacatctt gcggtgtcca actgcgtatt cgtggcaaag tgcagggcgt cggttttcgt
    50041 ccgtttgtct ggcagctggc acagcaatta aatcttcacg gcgatgtctg taatgacggc
    50101 gatggcgtag aagtccggct gcgggaagac ccggaaacgt ttcttgttca attgcatcag
    50161 cactgcccgc cgctggcgcg tattgatagc gtcgagcgtg agccgtacat ctggtcacaa
    50221 ctgcccactg agttcacaat ccgccagagc gcgggaggtg ccatgaatac gcaaattgtc
    50281 ccggatgccg ctacttgccc tgcttgcctt gccgaaatga ataccccagg cgaacggcgt
    50341 tatcgttatc cgtttatcaa ctgtacccac tgcggcccgc gtttcaccat tattcgcgcc
    50401 atgccttacg accgcccgtt taccgtgatg gcggcgtttc cgttgtgtcc ggcttgtgac
    50461 aaagagtacc gcgacccgct cgatcgtcgc ttccacgccc agccggtggc ctgcccggag
    50521 tgtggcccgc atcttgaatg ggtaagtcat ggtgaacatg cagaacaaga ggcggcatta
    50581 caggcggcta tcgcacagtt aaaaatgggc aacattgtcg ccatcaaagg gattggcgga
    50641 tttcatcttg cctgcgatgc acgtaacagt aacgcggtgg cgacacttcg ggcgcgcaaa
    50701 catcgcccgg cgaaaccgct ggcggtcatg ttgccagtgg ctgacggttt accagacgct
    50761 gcgcgccagt tgcttaccac gcccgccgcg ccgattgtgc tggtggataa aaaatacgtt
    50821 cctgagcttt gtgatgatat cgcccctgac cttaacgaag tcggggtaat gttgcctgcg
    50881 aacccgctcc agcatttgct gttacaggaa ctgcaatgcc cgctggtgat gacctccggc
    50941 aacctgagcg gtaaaccacc agctatcagc aacgaacagg cgctggcgga tttgcagggc
    51001 attgccgacg gattcttgat acataaccgc gacatcgtgc agcggatgga tgattcggtg
    51061 gtgcgcgaaa gcggcgaaat gctgcgccgt tcgcgggggt atgtgccgga tgcgctggct
    51121 ttgcctccgg gctttaaaaa tgttccgcct gtgctgtgtc tcggcgcgga tctgaaaaat
    51181 accttctgcc tggtgcgcgg tgaacaagcg gtgttgagtc agcatctggg cgatttaagt
    51241 gacgatggca tccagatgca gtggcgcgaa gcgttacgcc tgatgcaaaa catctacgat
    51301 tttaccccgc aatacgttgt gcatgacgcg catccgggct atgtctccag ccagtgggcg
    51361 cgtgaaatga atctgccgac gcaaacggta ctgcatcatc atgcccatgc agcggcgtgt
    51421 ctggcagagc atcagtggcc gctggatggc ggtgatgtca ttgctttgac gctcgacggt
    51481 atcggtatgg gggagaacgg cgctttgtgg ggcggcgagt gcctgcgggt gaactatcgc
    51541 gaatgtgagc acctgggcgg cttgcctgca gtggcgcttc cgggtggcga tttggcagcg
    51601 aagcagccgt ggcgaaacct gctggcgcag tgcctgcgct ttgtgccgga gtggcagaat
    51661 taccctgaaa cagcaagtgt gcaacagcaa aactggagcg tgctggcgcg ggccattgag
    51721 cgtggaatta acgcgccgct ggcgtcatcg tgtgggcggt tgttcgatgc agtggcggcg
    51781 gcactgggct gtgcgccagc cacgttaagt tatgaaggtg aagcggcttg tgctctggag
    51841 gcgctcgcag cctcatgcca cggagtgacg catccggtga cgatgccgct ggtggacaat
    51901 caactggatc tcgccgcttt ctggcagcag tggctgaact ggcaggcacc ggttaatcaa
    51961 cgcgcgtggg cgtttcatga tgcgctggcg cagggttttg ccgcgttgat gcgtgagcag
    52021 gccacgatgc gtggtatcac tacgctggta tttagcggcg gggttattca taaccgtttg
    52081 ctgcgtgcac gtctggcgca ttatctcgct gatttcacat tgctctttcc acagagttta
    52141 ccggcgggtg atggcggttt gtctctgggg cagggggtta ttgctgcggc gcgttggtta
    52201 gcgggtgaag tccagaacgg ataaaacgtg ttgcatttgg acaccattgc cggatgcggc
    52261 gtaaacgcct tatccggcct acacatgcgt ccccgtaggt cggataagac gcgccagcgt
    52321 cgcatccgac attttaggca cagtagccca cctacatcgg caatgttttc aacaatccaa
    52381 atgcctcttt catccgatcc tcactgacca caaaggcgcg aagctggtca gcatcgtcaa
    52441 caccgcgagc caccattccc tggcgttcgg tattaatttg ccagcgtaaa tcctgacgct
    52501 gggtttcgcc tgccagatgc agcggtaatt ccggcgtttt gattttcacc agcatcgccg
    52561 gtagtttcag cggcgtgtta ttgccgagaa gattttttgc cagcaccatc gcgctaagtt
    52621 gaatcggctg caggaatggc agtacctgac cgttgatttc cgcgcaatcg cccagcgcat
    52681 aaatatcgtc attactggtt tgcagataac tatcgacgca aacgccgcga ttaatcgtca
    52741 gcccggcgcg tcgtgccagg gcggtttccg ggcgcagtcc ggtggcggca attaccgcat
    52801 ccacttcgat gctacgctgg cggtccagcg ttgccagaat gccagaatcc gttttttcca
    52861 gcccctgtaa ctgagatttc aacagcagat gaacgcccat ctccgtcaac cgatgctgca
    52921 agcggctgct tacttccggt ggcattaacg acgccagaat actggcagcg ttgtcgatta
    52981 gcgtgaccgc tttgcctgca cgacaaaaat ccatcgccag ttcgctacca atcaaaccac
    53041 cgccaacaat caacacgcgt cgggcatccc gcagttgcgt ttcacaggcg cgatactctt
    53101 gctgactatt taacgtcagc attaactcac gcccaggcac aggcgggaca aaggcactgg
    53161 caccggttgc cagtactagc ttgtcgtatt gccactgatt attctggctt ttcaccacac
    53221 gggcttcggc atcgatatcc gtcacccagg tttgtggaaa caggtgcaga ttaaactgct
    53281 cggcaaattc acccgccgtc tggcgggtaa ggtcatcggc acgttgcccc tgactgataa
    53341 catggctgag gtcaggtttg ttgtactcat ccatgctgtc ggcggcaatc agggttaatg
    53401 gaatagtggc gtcctgtttg cgaatatttt tcaccagttg gcgggcggcg aagcccgaac
    53461 cgatgatcac aatgccgtta ctcattttgc ctccgatgcc agttcgtcaa agacgtcttt
    53521 gccgagggag cattccgggc agaggaagtt atccgggact tcactccacg gcgttcctgg
    53581 cgcaacgtcc tgcattggct cgccttttgc cggatcgtaa atccactggc agacgctgca
    53641 ctgcattcgt gggccgaggt cagccgtcgt cgtggcagag gtttcttctt taactaccgt
    53701 attcaccgtg ctctgcggca gcggcgcgag cgcccactga cgggcgattt cgcgaccgtg
    53761 ttcacggcat aactccagag cgtcctggtc tggtcgccat ttggctttca ggctgagcga
    53821 catttcgaaa cccgcatcct gcaggcgcgt ggaaaggcga tccaccgcac cgccgctcca
    53881 gccgtgagat ccgaaagcac tggcgcgttt gttacggaag cgtaatccgg tcatctcctc
    53941 caccagcccg gcgattttcg gcatcatcac gttattcatc gtcgaagtgc cgaccagcac
    54001 gccttttgag cggaagacat tggtcaggat ttcgttttta tcgcttcggg cgacgttgaa
    54061 aattttcacc gccacgcgtg ggtcggtttc cgcaattccc tgggcgatag cgtcagccat
    54121 catgcgggtg ttattcgaca tggtgtcgta gaaaatggtg attctgtctt cctgataatc
    54181 agcggcccac ttcaggtaca gctcgacaat ttgcgtcggg ttatcgcgcc ataccacgcc
    54241 gtgagaggtc gctatcatat cgactggtaa gttaaagccc aggatctcgg taattttcgg
    54301 tgttaccagg cggctgaacg gcgtcaggat attggcgtag taacgctggc actgctcgaa
    54361 aagctccgtc tgatccactt catcgttgaa cagatgctcg tcgcagtagt gctgaccgaa
    54421 agcatcgtta ctgaacagca ccgcgtcgcc tgtcaggtaa gtcatcatgc tgtccggcca
    54481 gtgcagcatt ggcgtttcga caaaaatgag ctgtttgccg ttgccgatat ccagcgtgtc
    54541 accagttttc accacattaa aattccactc cggatgatgg tgatgaccat ttatcgagtc
    54601 gatagcgttg gcagtacagt aaatcggcgt atcgggaatt tgcgccatca gttcggtcag
    54661 cgccccagcg tggtcctctt ctgcatggtt aatcacgatg taatcgatat ccgccagatc
    54721 gatttcatta cgcaggttct gcacaaattc gcggctgaat ttatggtcga cggtgtcgat
    54781 cagcacgttt ttttcttcgc ggatgaggta gctgttgtaa ctgctgccgc gcagcgtttt
    54841 atattccgta ccgtgaaaat cacgcacttc ccagtcacgt tgaccaaccc aatgaatgtt
    54901 atttttcacc acaatagaca tagcaacctc aatttattca gcgtgttcta aaaagatgtc
    54961 ttgctaattg cagattgcgt gccagttttt tatctgattg tttttactct ataaataaaa
    55021 ttatgcccat taatgagtag tcaaaatgac tatcaatcaa atggtcgata tgacaatatc
    55081 tatagtcaaa ttgacagtga ggcaaagatg agtttttccg ttgatgtgct ggcgaatatc
    55141 gccatcgaat tgcagcgtgg gattggtcac caggatcgtt ttcagcgcct gatcaccacg
    55201 ctacgtcagg tgctggagtg cgatgcgtct gcgttgctac gttacgattc gcggcagttt
    55261 attccgcttg ccatcgacgg tctggcaaag gatgtactcg gtagacgctt tgcgctggaa
    55321 gggcatccac ggcttgaagc gattgcccgc gccggggacg tggtgcgctt tcctgcagac
    55381 agcgaattgc ccgatcccta tgacggtttg attcctgggc aggagagtct gaaggttcac
    55441 gcctgcgttg gtctgccatt gtttgccggg caaaacctga tcggcgcact gacgctcgac
    55501 gggatgcagc ccgatcagtt cgatgttttc agcgacgaag agctacggct gattgctgcg
    55561 ctggcggcgg gagcgttaag caatgcgttg ctgattgaac aactggaaag ccagaatatg
    55621 atgccaggcg atgccacgcc gtttgaagcg gtgaaacaga cgcagatgat tggcttgtcc
    55681 cctggcatga cgcaactgaa aaaagagatt gagattgtgg cggcgtccga tctcaacgtc
    55741 ctgatcagcg gtgagacggg aaccggtaag gagctggtgg cgaaagcgat tcatgaagcc
    55801 tcgccacggg cggtgaatcc gctggtctat ctcaactgtg ctgcactgcc ggaaagtgtg
    55861 gcggaaagtg agttgttcgg gcatgtgaaa ggagcgttta ctggcgctat cagtaaccgc
    55921 agcgggaagt ttgaaatggc ggataacggc actctgtttc tggatgagat cggcgagttg
    55981 tcgttggcat tgcaggccaa gctgctgagg gtgttgcagt atggcgatat tcagcgcgtt
    56041 ggcgatgacc gtagtttgcg ggtcgatgtg cgcgtgctgg cggcgactaa ccgcgactta
    56101 cgcgaagagg tgctggcagg gcgatttcgc gctgacttgt ttcatcgcct gagcgtgttt
    56161 ccactttcgg tgccgccgct gcgtgagcgg ggcgatgatg tcattctgct ggcggggtat
    56221 ttctgcgagc agtgtcgttt gcggctgggg ctctcccgcg tggtattaag tgccggagcg
    56281 cgaaatttac tgcaacacta tcgttttccg gggaacgtgc gcgaactgga acatgctatt
    56341 catcgggcgg tagtgctggc gagagccacc cgcaacggcg atgaagtgat tcttgaggcg
    56401 caacattttg cttttcctga ggtgacgttg ccgccgccag aagcggcggc ggtgcccgtt
    56461 gttaagcaaa acctgcgtga agcgacagaa gcgttccagc gtgaaaccat tcgccaggca
    56521 ctggcacaaa atcatcacaa ctgggctgcc tgcgcgcgga tgctggaaac cgacgtcgcc
    56581 aacctgcatc ggctggcgaa acgtctggga ttgaaggatt aaataatccc ggcctgatag
    56641 aaatcctgca ggttaattgc gccggtgagt ttgccgtttt catccaccac cggtgcggca
    56701 gtgattttgc gcttcatcag gatctctttg gcgtcgatgg cgcgactttg cgcctgtaac
    56761 gtggtgccgc ctgtcgtcat cgcttcatta actggcgtgg tgagtgcgcc gccgcctacc
    56821 agccagcgac gtaaatcgcc gtcggtaaag acgccttgta cctgttgttg agcgtcacat
    56881 accgccacca gccccagacc ggtgcggctg agttccagca tcgcatccat cacgctggcg
    56941 gttaacgcca cctgtgggat ggcatcgtca cggcgcatca gatgatgcac tttattcagc
    57001 aagcgagcgc ccagtgcccc ggctgggtgg gagcgggcaa aatcttcttc attaaatccg
    57061 cgcgcctgca tgaccgccat cgccagcgcg tcacccatca tcagggtatt gacggtgctg
    57121 gaggtcggcg caaggtgcat cgggcaggct tcgcgttcta cggagatatc cagcaccgct
    57181 tttgccgcca ggcccagcgg tgacgtcggt ttgccggtca tcgccagcag cgcgatagat
    57241 ttatcttcca gacgcggaat aatcagatcc agttccttcg cgccaccgga gtaagagata
    57301 aacagcatca catcgcggct ttcaatcatt cccagatcgc cgtgcagtgc ttctgccgga
    57361 tggacaaaaa aagccggtgt gccggtactg gcaagtgttg cggcgatttt cttaccaatg
    57421 tggcccgatt tgccaattcc cgaaaccacc actttgcctt cgcagtgcag gatgatattg
    57481 gcggcgcgaa caaaatcatc gcccagacgt tccggtaaac ggcttgcttc ctgcaactcc
    57541 agcattaacg tctgacgtcc cgcgttcagt agtgcttcac tcattgctct ctccggttat
    57601 gatcacatcg atccctttct cttccagcgc ctgacgaaac gccggatcga tacctgcgtc
    57661 ggtaatcagc ttatcgacgc tttcaagact gcaaactacg ttggggcttt tacggccaaa
    57721 ctttgatgag tccgccatca aaatcacttc gcgcgcggca ttgcacattg ccttactgac
    57781 ggtataaacc tcgttaaagg tggttacgcc cgcattgaga tcgatgccgt cggtgcccat
    57841 aaacaattta tcgaaggtga aatgctcgaa ggcattctct gccagttgcc cgtgaaatga
    57901 ggccgatttt ttgcgaaacg ttccgcctgg catcaggata gtttgttcgt tatccagttc
    57961 ggatagcgca ttgacgatat gcaggctgtt ggtcatcacc gtgatgttat taaagcgcga
    58021 gagcagggga accatctgca aaacggtact gccagcatca agaatgatcg aatcgccatc
    58081 atggataaaa ctaacggcag cttctgcaat cagctctttc ttgtgggtgt tgatgagtgt
    58141 tttatgatcg ataggcggat cggattcctc tttattcaac accactccac cataagtacg
    58201 aatgacggtt ccggcatgtt ccagaatgac cagatctttg cgaatggttg tgcctgtggt
    58261 gtcaaagtat tgcgccaatt cttcaaccga gcatttaccc tgcttttgca gatactccag
    58321 aatggcggcc tgacgctgac gaggtttcat gggcgtgatt acctgattta ggtttcaaaa
    58381 tgataacttc gcaagcaagt aacattcaca acgaaattat ccacgtttca gtttaagcgc
    58441 caatgataga gcattctgtg atagtggatc atggggaaag atcacatcgg gctgtaaatc
    58501 acccgcatgc ataccggtaa ttgcgggaat tttttgcggt cgggcgaaga cggtcagtcc
    58561 tttcataaac aaggtgtcga cgatgcgctg ctgatcgtcc agcgcgatgg cgaccacgac
    58621 ccgcggttta aagcgcccgc tggaacggcc cacgccaacc cgcccttgct ggcatagtgt
    58681 gtcgaaggca cggttaaaac gagaaatttg ccagccgcct aaggccagtt gcgcacacca
    58741 ggcgataacg gcgacggtga tgagtgcgga taccataact tttccttaat gtagtggcgg
    58801 aagtcctgta tgcctgatga gcgtagcgca tcaggcattc tatagtctca cggagatcct
    58861 taatggatct ccgctgttga tcagaacatc acctgaccgc cggtgacatt gatcgactgt
    58921 ccggtgcagt acgacgcctt aggactggcg tagaacagca gcatattcag cacatcttga
    58981 taatcgcagc cgcgtttgag cggtactttg tcgatgtaat actgctcgac ttgatccggt
    59041 ttgataccca gcttggtcgc gtattgtggc aacagtgact ggaacatcgg cgatttcagc
    59101 aggttaccga gcatcagtga atgcaccgta atgccgtact ccgccagatc cagcgccagt
    59161 gattgagtca gcccgacgcc accaaatttc gctgcgctgt agccagagtt gtgtttgctg
    59221 cccactttgc cggatttcga gttgatctga ataatgcgac cctgaatccc gtcgcggatc
    59281 atcaaacgcg aaaattcacg ggcacacagg aaataaccca ccagattcac ttgtagcgaa
    59341 cggtcaaaat cgccgagctg gaagtcgctg ataaaggctg cttttgctat tccggcgcta
    59401 tagaccagca aatccacgcg accaaagatt tcatctaccc cacgagagag cgccagaacg
    59461 ctttgctcgc tagtagcgtc agcaccaaaa ccgtacgcca tactttcacc atattcggcg
    59521 ttaatttctt gtgccacatt tgcggctttg tcgctctgaa tatcgacaac cgcgacgcga
    59581 tacccctcgg cagccagacc gtggcacagg aacgcgccta aggtttgccc accaccgatg
    59641 acaacggcaa cctgattcat tttttactcc ttaacagatt caaacttcaa aaccgatccc
    59701 ggcgcgatat cgtcgggtac agggcctgcc acatggacag tgcccggaaa ttccgcttcg
    59761 tttaaaccat cgaagcgcag ggtgacatga cccagttcgc gaaggttgtc ttccgccacg
    59821 ctgccaacag cggtcaccgg atagcgatgc tgcccgagcg taaattgcaa accgggatgg
    59881 agtgcacctt tcaactcgcc gtggcaatga atgaagcaat actcttcgag gtccgctggc
    59941 gcgccttcac gaaaggtgat gagcatctgg tcgctgaggg cgtcagtggc actcgcgccg
    60001 atacgggtga tggtggtctg ataaataacg gtcatgtttc agcctctatt gatagataaa
    60061 accggagaca aaccaggcga tcagtacagt cggtgcgccg gttaaaaagc ggctcaccag
    60121 tacagaaggg acaccgacgc gaaccgtgtc ctgacgggct tccgccagcg acaaaccgac
    60181 cgggatgaag tcgcaggccg cctgcgcgtt gatggcaaac agtgccggta aagccagatg
    60241 cggcggaata ttgccgagac caatctgcac gccaatcaat acgccgataa cctgtgcgat
    60301 aactgcgcct gggccgagga aaggtgaaag cggtgggaag gagcagatga gcgccagcat
    60361 gaccagaccc agtggatggc tcgccagcgg agcaagaccg tgggcaatcc agtcaccaag
    60421 gccagaagcc ataatgatgc caatgagcgc cgagacgaat gccataaacg gcagaatggt
    60481 ttttaataca gtgtcgatgg tgtcacgacc agattgaaac agcaccgcaa cggcggaccc
    60541 catgcccatt cccaccttcg ccagtaaacc atcgctttgt tcggtgattt tcttactggt
    60601 gtcatagtca cggcccacgg aagagggttg tggtgtcgca tcacccacta cagtgatgtt
    60661 ttcttctttt acgccagaga cataaatatc ttccacaatg tactgcgcca gcggaccgga
    60721 cttgcccgtc gagtggatat taatggtggg aatacgtcgt ttcggataga tgccgcagcg
    60781 taatgtgccg ccacagtcga ttaccgcgac accaatttcc gcctccgcgg gttcaccttc
    60841 tttaaatccg tcaatagcct gccagccagt aagttgtgcc agtttgtcaa caatcgcagg
    60901 ccgggtaccg gcggtgatat agacgatttt tttgcccggc gtggcttcca gctcaagcgg
    60961 gccgccccag ccacccgttc ctttttcgat ccgaatacgc gtcatgatgt tgctcctgcc
    61021 aggtgaactt tttgttcaag ttgaatgccc atctttttct caaaaatggc ggtggtcaga
    61081 tcggtcaccc agccgcgaaa gaagttggtg accagaccaa ccagcagata gctcaccgcc
    61141 agtgggccaa gtggcaggtt cagcgttgtc agaccgctgg caatgccaag ataaacaaac
    61201 agttcgccag ggttgatatg gggaaagagg ccattcattg agtggcagct ataagaggcc
    61261 gccgcgtagt agctgggttt gtacttttcc ggcataaagc gacccaggct taaggtcatc
    61321 ggattgcaaa agacaaacgt gccaatgcac ggtagcagca ggtaacggga aacagggtta
    61381 ccggcgcaac gttgagcaaa acgttcaata cgatgctgac cgataaaatt aatcagtgcg
    61441 ttcatgataa ccagcaggct aatcaacagc ggaagaatgc cggtcaccat cccggtaaac
    61501 acctctccgc ccttttggaa cagcccgata aaccactctg caccatgagt aatggtttct
    61561 atcattgttc tctccttcag gatttattgt tttattacca aacggcaacc taatctaatc
    61621 agattgaaag atctaaaagt gttattttga tcgcaaaatg aaagataaat attttaattt
    61681 gaaagtttga ataaaaggat agcgagggga atgagttgag ttatgtaaag tccgtatcgg
    61741 gcagtgacta ccgcttcctt gtgcggggcg tgatgcttta ccatacttgc ccctggttga
    61801 atctgttaaa tggacccctc atgttcaagc gtcgttatgt aacattgctt cccctttttg
    61861 tgttgcttgc cgcctgtagc agcaagccaa aacctactga gactgaaacg accaccggaa
    61921 cgccgtctgg cggcttcctg cttgagccgc agcacaatgt gatgcagatg ggcggcgatt
    61981 tcgctaataa cccgaatgcc cagcagttca tcgacagaat ggtgaacaaa cacggtttcg
    62041 atcgtcagca gttgcaggaa attctctccc aggcgaagcg tctggattcg gtactgcggc
    62101 tgatggataa ccaggcacca accacatcgg tgaaaccgcc atcaggtccg aacggcgcat
    62161 ggctgcgtta tcgcaaaaaa tttattacgc cggacaacgt gcagaacggt gtggttttct
    62221 ggaatcagta tgaagatgcg ttgaatcgcg cgtggcaggt gtatggcgta ccgccggaaa
    62281 ttatcgtcgg gattatcggc gttgaaaccc gctgggggcg cgtgatgggg aaaactcgca
    62341 tcctcgatgc gctggcaacg ctgtcattta actacccacg ccgcgcggag tatttctctg
    62401 gcgagctgga aaccttcctg ttgatggcgc gcgacgagca ggacgatccg ctcaatctga
    62461 aaggttcctt tgccggggcg atgggctacg gacagtttat gccgtcgtct tacaaacaat
    62521 atgcggtaga tttcagcggc gacgggcata tcaacctgtg ggatccggtt gatgcgatcg
    62581 gtagcgtggc gaactatttc aaagcgcacg gctgggtgaa aggcgatcag gtcgcggtaa
    62641 tggcaaacgg tcaggctcca ggcttgccaa atggcttcaa aactaagtac agcatttcgc
    62701 agcttgccgc cgcaggttta acgccacagc agccgctggg caaccatcaa caagccagcc
    62761 tgctgcgtct ggatgttggc accggctacc agtactggta cggtctgccg aacttctaca
    62821 ccatcacccg ttacaaccac agcacccatt acgcaatggc ggtctggcag ttaggacaag
    62881 ccgtggcgct ggcgcgagta cagtagctat ttacaagagg ggggcgtgag cttccctctg
    62941 gctaatcagc ttttttctga atcctcctcg taaaattgca acgccaacac catcttcctg
    63001 acgaaagtgc tatcttgtcc ggcataaatt tttgactgac agaggttgtg atgactgaca
    63061 gtgaactgat gcagttaagt gaacaggttg ggcaggcgct gaaagcccgt ggcgcaaccg
    63121 taacaactgc cgagtcttgt accggcggtt gggtagcgaa agtgattacc gatattgccg
    63181 gtagctccgc ctggtttgaa cgcggatttg tcacctacag taacgaagcc aaagcgcaga
    63241 tgatcggcgt acgcgaagag acgctggcgc agcatggcgc ggtgagtgaa cccgtcgtgg
    63301 tggaaatggc gataggtgca ctgaaagcgg ctcgtgctga ttatgccgtg tctattagtg
    63361 gtatcgccgg gccggatggc ggcagtgaag agaagcctgt cggcaccgtc tggtttgctt
    63421 ttgccactgc ccgcggtgaa ggcattaccc ggcgggaatg cttcagcggt gaccgtgatg
    63481 cggtgcgtcg tcaggctact gcgtatgcat tgcagacctt gtggcaacaa tttctacaaa
    63541 acacttgata ctgtatgagc atacagtata attgcttcaa cagaacatat tgactatccg
    63601 gtattacccg gcatgacagg agtaaaaatg gctatcgacg aaaacaaaca gaaagcgttg
    63661 gcggcagcac tgggccagat tgagaaacaa tttggtaaag gctccatcat gcgcctgggt
    63721 gaagaccgtt ccatggatgt ggaaaccatc tctaccggtt cgctttcact ggatatcgcg
    63781 cttggggcag gtggtctgcc gatgggccgt atcgtcgaaa tctacggacc ggaatcttcc
    63841 ggtaaaacca cgctgacgtt gcaggtgatc gccgcagcgc agcgtgaagg taaaacctgt
    63901 gcgtttatcg atgctgaaca cgcgctggac ccaatctacg cacgtaaact gggcgtcgat
    63961 atcgataacc tgctgtgctc ccagccggac accggcgagc aggcactgga aatctgtgac
    64021 gccctggcgc gttctggcgc agtagacgtt atcgtcgttg actccgtggc ggcactgacg
    64081 ccgaaagcgg aaatcgaagg cgaaatcggc gactctcaca tgggccttgc ggcacgtatg
    64141 atgagccagg cgatgcgtaa gctggcgggt aacctgaagc agtccaacac gctgctgatc
    64201 ttcatcaacc agatccgtat gaaaattggt gtgatgttcg gtaacccgga aaccactacc
    64261 ggtggtaacg cgctgaaatt ctacgcctct gttcgtctcg acatccgtcg tatcggcgcg
    64321 gtgaaagagg gcgaaaacgt ggtgggtagc gaaacccgcg tgaaagtggt gaagaacaaa
    64381 atcgctgcac cgtttaaaca ggctgaattt cagatcctct acggcgaagg tatcaacttc
    64441 tacggcgaac tggttgacct gggcgtaaaa gagaagctga tcgagaaagc aggcgcgtgg
    64501 tacagctaca aaggtgagaa gatcggtcag ggtaaagcga atgcaactgc ctggctgaaa
    64561 gataacccgg aaaccgcgaa agagatcgag aagaaagtac gtgagttgct gctgagcaac
    64621 ccgaactcaa cgctggattt ctctgtagat gacagcgaag gcgtagcaga aactaacgaa
    64681 gatttttaat cgttcgttat tgatgataat agataatgtg tgcacggacg gtcccctcgc
    64741 ccctttgggg agagggttag ggtgagggga acaggccagc actggcgtga aaaattactc
    64801 tcacctcgat cctctaacga attgggcctg cgggaacatt ctgcacactc attgactatt
    64861 aaatctgttt atcagcaaaa cctgggtcgc atctgcggcc cttttgcttt tttaagttgt
    64921 aaggatatgc catgacagaa tcaacgtccc gtcgcccggc atatgctcgc ctgttggatc
    64981 gtgcggtacg cattctggcg gtgcgcgatc acagtgagca agaactgcga cgtaaactcg
    65041 cggcaccgat tatgggcaaa aatggcccag aagagattga tgctacggca gaagattacg
    65101 agcgcgttat tgcctggtgc catgaacatg gctatctcga tgacagccga tttgttgcgc
    65161 gctttatcgc cagccgtagc cgcaaaggtt atggacctgc acgtattcgt caggaactga
    65221 atcagaaagg tatttcccgc gaagcgacag aaaaagcgat gcgtgaatgt gacatcgact
    65281 ggtgcgcact ggcgcgcgat caggcgacgc gaaaatatgg tgaacctttg ccaactgtct
    65341 tttcagaaaa agttaagatc cagcgttttc tgctctatcg tggctatctg atggaagata
    65401 tccaggatat ttggcgaaat tttgccgact gaacgcatac ggtattttac ttcccagtca
    65461 agaaaactta tcttattccc acttttcagt taccagcccg gcggttaaga cacgctggag
    65521 ctggtggcga tatttcgtta gcttgatttc aggataatta tgagcaagag caccgctgag
    65581 atccgtcagg cgtttctcga ctttttccat agtaagggac atcaggtagt tgccagcagc
    65641 tccctggtac cccataacga cccaactttg ttgtttacca acgccgggat gaaccagttc
    65701 aaggatgtgt tccttgggct cgacaagcgt aattattccc gcgctaccac ttcccaacgc
    65761 tgcgtgcgtg cgggtggtaa acacaacgac ctggaaaacg tcggttacac cgcgcgtcac
    65821 cataccttct tcgaaatgct gggcaacttc agcttcggcg actatttcaa acacgatgcc
    65881 attcagtttg catgggaact gctgaccagc gaaaaatggt ttgccctgcc gaaagagcgt
    65941 ctgtgggtta ccgtctatga aagcgacgac gaagcctacg aaatctggga aaaagaagtc
    66001 gggatcccgc gcgaacgtat tattcgcatc ggcgataaca aaggtgcgcc atacgcatct
    66061 gacaacttct ggcagatggg tgacactggt ccgtgcggcc cgtgcaccga aatcttctac
    66121 gatcacggcg accacatttg gggtggccct ccgggaagtc cggaagaaga cggcgaccgc
    66181 tacattgaga tctggaacat cgtcttcatg cagttcaacc gccaggcaga tggcacgatg
    66241 gaaccgctgc cgaagccgtc tgtagatacc ggtatgggtc tggagcgtat tgctgcggtg
    66301 ctgcaacacg ttaactctaa ctatgacatc gacctgttcc gcacgttgat ccaggcggta
    66361 gcgaaagtca ctggcgcgac cgatctgagc aataaatcgc tgcgcgtaat cgctgaccac
    66421 attcgttctt gtgcgttcct gatcgcggat ggcgtaatgc cgtccaatga aaaccgtggt
    66481 tatgtactgc gtcgtatcat tcgtcgcgca gtgcgtcacg gcaatatgct cggcgcgaaa
    66541 gaaaccttct tctacaaact ggttggtccg ctgatcgacg ttatgggctc tgcgggtgaa
    66601 gacctgaaac gccagcaggc gcaggttgag caggtgctga agactgaaga agagcagttt
    66661 gcccgtactc tggagcgcgg tctggcgttg ctggatgaag agctggcaaa actttctggt
    66721 gatacgctgg atggtgaaac ggctttccgt ctgtacgaca cctatggctt cccggttgac
    66781 ctgacggctg atgtttgtcg tgagcgcaac atcaaagttg acgaagctgg ttttgaagca
    66841 gcaatggaag agcagcgtcg tcgcgcgcgc gaagccagcg gctttggtgc cgattacaac
    66901 gcaatgatcc gtgttgatag tgcatctgaa tttaaaggct atgaccatct ggaactgaac
    66961 ggcaaagtga ccgcgctgtt tgttgatggt aaagcggttg atgccatcaa tgcaggccag
    67021 gaagctgtgg tcgtgctgga tcaaacacca tactatgcgg aatccggcgg tcaggttggt
    67081 gataaaggcg aactgaaagg cgctaacttc tccttcgtgg tggaagatac gcagaaatac
    67141 ggccaggcga ttggtcacat cggtaaactt gctgcgggtt ctctgaaagt gggcgacgct
    67201 gtgcaggctg atgttgatga ggctcgtcgc gcccgtattc gtctgaatca ctccgcaacg
    67261 cacctgatgc acgctgcgct gcgccaggtt ctggggactc atgtatcgca gaaaggttca
    67321 ctggttaacg acaaggtgct gcgcttcgac ttctcacaca acgaagcgat gaaaccggaa
    67381 gagattcgtg cggtcgaaga cctggtgaac gcacagattc gtcgcaattt gccgatcgaa
    67441 accaacatca tggatctcga agcggcgaaa gcgaaaggtg cgatggcgct gttcggcgag
    67501 aagtatgatg agcgtgtacg cgtgctgagc atgggcgatt tctccaccga gctgtgtggc
    67561 ggtactcacg ccagccgcac tggtgatatt ggtctgttcc gcatcatctc tgaatcgggt
    67621 actgctgcag gcgttcgtcg tatcgaagcg gtaaccggag aaggcgctat caccaccgtt
    67681 catgcagaca gcgatcgctt aagcgaagtc gcgcatctgc tgaaaggcga tagcaataat
    67741 ctggcggata aagtgcgctc agtactggaa cgtacgcgtc agttagaaaa agaactacaa
    67801 cagcttaaag aacaagctgc cgcacaggag agcgcaaatc tttccagtaa ggcaattgat
    67861 gttaatggtg ttaagctgtt ggttagcgag cttagcggtg ttgagccgaa aatgttgcgt
    67921 accatggttg acgatttaaa aaatcagctg gggtcgacaa ttatcgtgct ggcaacggta
    67981 gccgaaggta aggtttctct gattgcaggc gtatctaagg acgtcacaga tcgtgtgaaa
    68041 gcaggggaac tgattggtat ggtcgctcag caggtgggcg gcaagggtgg tggacgtcct
    68101 gacatggcgc aagccggtgg tacggatgct gcggccttac ctgcagcgtt agccagtgtg
    68161 aaaggctggg tcagcgcgaa attgcaataa tataagcgtc aggcaatgcc gtggactcgc
    68221 ttcacggcat tcgcattaac gctatcgaca acgataaagt caggttgaag ttgtgtatat
    68281 cggctaaact taggtttaac agaatgtaat gccatgactg cttacatgta atgtgtttgt
    68341 cattgcttac tttttggcgt tatatgatgg ataatgccgg gatacagaga gacccgactc
    68401 ttttaatctt tcaaggagca aagaatgctg attctgactc gtcgagttgg tgagaccctc
    68461 atgattgggg atgaggtcac cgtgacagtt ttaggggtaa agggcaacca ggtacgtatt
    68521 ggcgtaaatg ccccgaagga agtttctgtt caccgtgaag agatctacca gcgtatccag
    68581 gctgaaaaat cccagcagtc cagttactaa tctttccgcg tctcatcttt atcggtgaga
    68641 cgcaccctca aaatttctcc ttcactctat agtcttttcg ctttactccc gttcattcaa
    68701 cttaagtctc catttttttg cattactgct atctgccaga cctccgtttt tctgtagata
    68761 aaacactctt tttgacgttt ttacagacta attgagcgtg aagtgtgcaa acgataaaag
    68821 tgtgggaaaa attgtttgac ttataagtct cagaaagtaa tatgtgcgcc acgcagcgac
    68881 gatgagcgat aaacaagttc ttcgaagcac tcgtaagagg cgtgtggtga ggtggccgag
    68941 aggctgaagg cgctcccctg ctaagggagt atgcggtcaa aagctgcatc cggggttcga
    69001 atccccgcct caccgccatt tgcatccgta gctcagctgg atagagtact cggctacgaa
    69061 ccgagcggtc ggaggttcga atcctcccgg atgcaccata ttctacgtac tttcagcgat
    69121 gaaggtatgg aagaggtggc ggtataaccg caggcactag ggaggataac gttgctttag
    69181 caacggcccg aagggcgagc cgcaaggcga gtaatcctcc cgaatgcacc atctcttact
    69241 tgatacggct ttagtagcgg tatcaaaaaa tctgcagtaa agtaagtttc ccgatgcatc
    69301 cgtagctcag ctggatagag tactcggcta cgaaccgagc ggtcggaggt tcgaatcctc
    69361 ccggatgcac catctcttac ttgatatggc tttagtagcg gtatcaatat cagcagtaaa
    69421 ataaattttc ccgatgcatc cgtagctcag ctggatagag tactcggcta cgaaccgagc
    69481 ggtcggaggt tcgaatcctc ccggatgcac catattctcc gaaatcttca gcaatgaagg
    69541 tatcgaagag gtagcagaat taaccgcagg caccagggat gataacgttg cgtcagcaac
    69601 ggcccgaagg gcgaacgcca gtgagtcatc ctcccggatg caccatcttc tcccaaaaat
    69661 ttccttcaaa aatatctcat ttctaaataa ctactttatc catttcctga attttcttct
    69721 ccttcgccat cagcgacaaa atcaagcaat tacgctaaag tagcgtcata tttttcgact
    69781 tgtgagaaga ccatgtacga gcgttatgca ggtttaattt ttgatatgga tggcacaatc
    69841 ctggatacgg agcctacgca ccgtaaagcg tggcgcgaag tattagggca ctacggtctt
    69901 cagtacgatg ttcaggcaat gattgcgctt aatggatcgc ccacctggcg tattgctcag
    69961 gcaattattg ggctgaatca ggccgatctc gacccgcatg cgttagcgcg tgaaaaaaca
    70021 gaagcagtaa gaagtatgct gctggatagt gtcgagccgc ttcctcttgt tgaagtggtg
    70081 aaaagttggc atggtcgtcg cccaatggct gtaggaacgg ggagtgaaag cgccatcgct
    70141 gaggcattgc tggcgcacct gggattacgc cgttattttg acgccgtcgt cgctgctgat
    70201 cacgtcaaac accataaacc cgcgccagac acatttttgt tgtgcgcgca gcgtatgggc
    70261 gtgcaaccga cgcagtgtgt ggtctttgaa gatgccgatt tcggtattca ggcggcccgt
    70321 gcagcaggca tggacgccgt ggatgttcgc ttgctgtgag tgaagcgtta tcgcttttct
    70381 cgttgtttgc cagtagtttt ctcagcgcta cactattacc cggcaactcc gaagtcgtgc
    70441 tggtggcaat gttgctttcc gggatcagtt atccctgggt tttagtttta acagcaacaa
    70501 tgggtaatag ccttggaggg ttaactaacg ttatccttgg gcgtttcttt ccattgcgta
    70561 aaacatcgcg ctggcaagag aaagctactg gctggctgaa acgctatggt gcagtcacgc
    70621 tattattaag ctggatgccc gtggttggcg atttactgtg tctgttagcg ggatggatgc
    70681 gcatctcgtg gggaccggta atcttttttt tgtgccttgg taaagcgtta cgctatgttg
    70741 cagttgcagc agctaccgtt cagggcatga tgtggtggca ctaattgtag gcctgcacat
    70801 atggtcacca ttacagttat gctaattaaa acgattttga caggcgggag gtcaatttga
    70861 tcccggacgt atcacaggcg ctggcctggc tggaaaaaca tcctcaggcg ttaaagggga
    70921 tacagcgtgg gctggagcgc gaaactttgc gtgttaatgc tgatggcaca ctggcaacaa
    70981 caggtcatcc tgaagcatta ggttccgcac tgacgcacaa atggattact accgattttg
    71041 cggaagcatt gctggaattc attacaccag tggatggtga tattgaacat atgttgacct
    71101 ttatgcgcga tctgcatcgt tatacggcgc gcaatatggg cgatgagcgg atgtggccgt
    71161 taagtatgcc atgctacatc gcagaaggtc aggacatcga actggcacag tacggcactt
    71221 ctaacaccgg acgctttaaa acgctgtatc gtgaagggct gaaaaatcgc tacggcgcgc
    71281 tgatgcaaac catttccggc gtgcactaca atttctcttt gccaatggca ttctggcaag
    71341 cgaagtgcgg tgatatctcg ggcgctgatg ccaaagagaa aatttctgcg ggctatttcc
    71401 gcgttatccg caattactat cgtttcggtt gggtcattcc ttatctgttt ggtgcatctc
    71461 cggcgatttg ttcttctttc ctgcaaggaa aaccaacgtc gctgccgttt gagaaaaccg
    71521 agtgcggtat gtattacctg ccgtatgcga cctctcttcg tttgagcgat ctcggctata
    71581 ccaataaatc gcaaagcaat cttggtatta ccttcaaaga tctttacgag tacgtagcgg
    71641 gccttaaaca ggcaatcaaa acgccatcgg aagagtacgc gaagattggt attgagaaag
    71701 acggtaagag gctgcaaatc aatagcaacg tgctgcagat tgaaaacgaa ctgtatgcgc
    71761 cgattcgtcc aaaacgcgtt acccgcagcg gcgagtcacc ttctgatgcg ctgttacgtg
    71821 gcggcattga atatattgaa gtgcgttcgc tggacatcaa cccgttctcg ccgattggtg
    71881 tagatgaaca gcaggtgcga ttcctcgacc tgtttatggt ctggtgtgcg ctggctgatg
    71941 caccggaaat gagcagtagc gaacttgcct gtacgcgcgt taactggaac cgggtgatcc
    72001 ttgaaggtcg caaaccgggc ctgacgctgg gtatcggctg cgaaaccgcg cagttcccgt
    72061 tactgcaggt gggtaaagat ctgttccgcg atctgaaacg cgtcgcgcaa acgctggata
    72121 gcattaacgg cggcgaagcg tatcagaaag tgtgtgatga actggttgcc tgcttcgata
    72181 atcccgatct gactttctct gcccgtatct taaggtctat gattgatact ggtattggcg
    72241 gaacaggcaa agcgtttgca gaagcctacc gtaatctgct gcgtgaagag ccgctggaaa
    72301 ttctgcgcga agaggatttt gtagccgagc gcgaggcatc tgaacgccgt cagcaggaaa
    72361 tggaaaccgc tgataccgaa ccgtttgcgg tgtggctgga aaaacacgcc tgacagaaaa
    72421 gaaaaaggcc actcgtgagt ggccaaaatt tcatctctga attcagggat gatgataaca
    72481 aatgcgcgtc tttcatatac tcagactcgc ctgggaagaa agagttcaga aaattttaaa
    72541 aaaaattacc ggaggtggct aaatgccgtt gttagatagc ttcacagtcg atcatacccg
    72601 gatggaagcg cctgcagttc gggtggcgaa aacaatgaac accccgcatg gcgacgcaat
    72661 caccgtgttc gatctgcgct tctgcgtgcc gaacaaagaa gtgatgccag aaagagggat
    72721 ccataccctg gagcacctgt ttgctggttt tatgcgtaac catcttaacg gtaatggtgt
    72781 agagattatc gatatctcgc caatgggctg ccgcaccggt ttttatatga gtctgattgg
    72841 tacgccagat gagcagcgtg ttgctgatgc ctggaaagcg gcaatggaag acgtgctgaa
    72901 agtgcaggat cagaatcaga tcccggaact gaacgtctac cagtgtggca cttaccagat
    72961 gcactcgttg caggaagcgc aggatattgc gcgtagcatt ctggaacgtg acgtacgcat
    73021 caacagcaac gaagaactgg cactgccgaa agagaagttg caggaactgc acatctagtc
    73081 agtaaactat cttcacaatt aattgaaaaa agccagttca aatgaactgg cttagttgta
    73141 cttagtgcgc accgcctccg ccgccacctg cgccaaatgg cggtttagca aaccacacca
    73201 gccccagcag tacgaggaat atcccggctg acatccagaa gatctcattg gcggaaataa
    73261 tcagcccctg attggtgatc tgctgggcaa tccagcctga tgcctgctgt tgcgtcatcc
    73321 caagcccttc cagttgactg tacatcgcct gggcattcgg gttgaacggg tttaccgact
    73381 cagtcaactg cgcatggtgc atcgactcgc ggttggtcca catggtcgtg gttatcgacg
    73441 tgccgataga ccccgccagc gttcgcgtaa agttggagag gctcgatgcc gccgccagtc
    73501 gttccggtgg caaaccagac agcgtaatgg tggtcagcgg cataaagaag caggccaccg
    73561 caaacccctg gataaactgc ggccaggccg acgcgccaaa atccatacct ggttcaaagg
    73621 tataggcacg ccagtagaag cagacggcat acataataaa gctgaaggtt accagccgcc
    73681 gcatatccag tttatgcgcg aagcggccga taatcggcga caggatcacc ggaataatcc
    73741 ctaccggcgc agaggccaaa cctgcccagg tcgccgtgta accgtagacc tcctgcaaca
    73801 actgcggcag cagaacaata gcgccgaagt agagcatata cgcgaggctg atacacaagc
    73861 agccgatggt gaagttgcgc gacttaaaca acgacagatc gactatcggg ttatcgtcgg
    73921 tcagctccca gacaatcagg aagcagatag ccaccaccgc caccacggta agaatgataa
    73981 tttcctgtga tgaaaaccag tccagctctt taccgcggtc gagcataatc tgcaggctgc
    74041 cgataccaat aaccagcagt gccagcccca cggcatcaat ccgccgccgt tcggtgcggg
    74101 tttcacgtcc gcgcagagtt tgcagtgtca tcaacaccac cgccacgcca atcggcacgt
    74161 tgatgaagaa tatccagccc cagtggtaat tatcgctgat ataaccgccg aggatcgggc
    74221 cgcaaattgg cgcgacaatc accgtcatcg accacaacgc cagcgcgatc gagcgtttgg
    74281 ctggcgggta gttattcagc aatagacttt gcgaaagcgg gatcaacggc ccggcgacaa
    74341 tcccctgaat cacgcggaag aagatcagca tattcaggct gctggagaca ccacacgccc
    74401 acgacgcaat agcaaaggcg atggtggacc aaaggaacag tttcacttcc ccgacgcgct
    74461 ttgccagcca gccggtaagc gggatcgaga tggcattcgc caccccgaaa gaagtgatta
    74521 cccacgttcc ctggctgagc gatgagccca gattcccggc gatagtgggg atcgccacgt
    74581 tagcaatggt ggagtccagc acctgcatga atgtcgccag tgacagcgca atcgtcataa
    74641 tgaccagttg cgcgccttcc agcggttttt gctgttgcat cacacgcacc tctggattag
    74701 ccagcgttag cttttacgat atcgtcgatc agtttattga caggtgccag gctgatttca
    74761 cgcgcggtac ttaccgcgac cggtgttgaa cgtactttat ttgccagtac ctgaccgtca
    74821 cggttagtgg tattgacgct caccagcgtg gacaaaccaa tgcgcagcgg gtattgctcc
    74881 agctgtttct ggtccagttc gatacgcaca ggtagacgct gaacgacttt gatccagtta
    74941 ccggtcgcat tttgcgccgg aagcagtgag aacgcgctac ctgtgcccat atccagacca
    75001 accactttac cggtgtattt cacatcatcg ccgtaaatat cggtggtgat ggtgaccggc
    75061 tgaccgatgc gcatattggc aatctgcgtc tctttaaagt tggcatccac ccacatattg
    75121 gtggctggaa cgaccgccat cagcggcgtc gttgggctaa tttgcgcccc aggctgtacc
    75181 gcgcggcggg agacataacc ggtcatcgga ctgacaatac gggtacgctc cagcgccagc
    75241 caggcgttac gtacttcggt ggcagcctgt tgcacggcag gctgatcttc cagtttagtc
    75301 cccagaatca tcgcctgatt ggcattgtat tgttgaatag cgacgtccag ttgcgcctgg
    75361 gcactggtga cggcgtcgcg ggcgtgttgc agctcttcgc gaccaatcag gttggcattg
    75421 cccagcggca cacggcggtt gtagtcgctt tgtgctttcg cgagggcgat tttctgcacc
    75481 tcaatattcg cctgcaactg tttgctgtta atcatcagct ggtgggtttg gcgaacgctg
    75541 gaagccagtg cagttttggc tttttcaaac gcctggcgag catctgtcgg gtcgagagtg
    75601 accagcacgt cgccttcttt tacaaaatcg gtgttatcgg cccagacttt cgtcacgctg
    75661 ccagacacct gagacataat ttgaatttga ttccctgcca cgtatgcgtc atcggtttct
    75721 tcgaaatgac gcagtaccaa aaaccaataa atccctatcg ctacggcaat aattataaag
    75781 agcaaggtga gaaggaggag cagacgctta cgtttgccgc tcttctttac cggttgctgc
    75841 ggggtttgag tctccgcatt tgcgctcata ttgttctcca cgatcttctt aactcatcgg
    75901 ctgagccgac ctgcttgcca aaaaggccag catgttgcga tgctggccgg tcattttttc
    75961 ttttataaat ctggattttt gagcgagatg acgcgttagc tcatcgcttc gagaaccaca
    76021 ccgtcttgtt ccatctggtc gagacgggag agcaatttgc gggtgatttg ctcgagctga
    76081 tctttttctg ttgtgctgag cgcggaccag agttgatgca ggcagttatg ctgcggtggt
    76141 aaaacctcgc gcaaaaactc gtgacctttt tccgttaatt gcagatgcag gcagcggcga
    76201 tcgttatcgc tttcacgacg ttcgatccaa ccgcgttttt ccagttcatc ggcaatacgc
    76261 gtcgcgttgg tacgggatga tccaagagca caacttaatt cagaaggctg aatactgtgg
    76321 ttttcctgag actccagcgt aatcaacgcc ataaacaacg tctcgttaat cccctgagcc
    76381 ttcagcattt tattgcggtt ctccagcagc ttgctttgca tgtgcatgca aagacgagtc
    76441 agaaggatct cctgataagg aaaatcttcg tggcggctgg cgcgaaattt tagcatttgt
    76501 tcaatgggcg taaacgaact atccatttgg gtatgacctc attaatttca gccgatatag
    76561 taacgacagt gacaaataaa gtaaatgtat tgttttagaa aaatgattct tgtgggttat
    76621 ataatcgcca tcactttcca ggcgagccca taggccagcg cactaagcag tgttgggatg
    76681 ataatgctgc gtgttttata gaaactggca cccagtaccg cgaagccgac cagcgtgggc
    76741 acgaaacggc gtgtatcgtg catcacttct ggtgcggtag agacaaccag cagagcgcat
    76801 atcgaggcga tgccaatggt gtcgagcaaa atacctaccg cgccacgttt ggttgggcgg
    76861 gcattaccca cacgcaggcg cagcggcaaa tagcggaagc aataattcgc tacgccaacc
    76921 agtaacccaa gcagcagaac ctcatagctc atcgggcgct ccttgccaga atgcctggat
    76981 taacgcagtg aggcagccac agacaatgcc tgccagaatg gcgacgggaa tagaaaatag
    77041 cgttacgcct gcaagggcac caactaacgc tgcggtaacg caaagagatt gtttgcgctg
    77101 gaaagaggcg agcaggaaac tcataaagag tgccggaagc ataaaaccta atgcagcttc
    77161 aacggcggga taaccttgca gcaagccgct gccggagaat gcccctatta ccgtaccaaa
    77221 tacccacgat gaccatgaac tgaaggcaat gccgatcatc cagttctcgc tccagcggcg
    77281 attattgcgt accagttttg cggttgcggc ggcaaaaacc tcatccgtca ggccaaacgc
    77341 ccacagggcg gttttcgatt tttgcagacg ctgaataata cggctacgca gtgacgggcc
    77401 atacaacaca tggcgaacat ccattgccat gacggtcagt gcagcaatcc acaaactact
    77461 cccggctgcc agcatcgcgg taatgacgaa ctggctcgcg cctgcataaa tgatgcagga
    77521 gaaaaaaacg ctttcgagag gagagaatcc cagacgggtc gcattcagac cgaacgcaaa
    77581 ggccaccgga atataactaa taacaatcgg taaactgtct ttgcatcctt ccatgaaggt
    77641 cgccgaacca ggagcaggct gtggagtagg gctttccata gagtgtacgc ttaacgattg
    77701 ttaatattat aaatagactg aatgaatatc ttaaccttat cagactgatg ggcttcttaa
    77761 cacccttata agtgtaaagc cacgaaaacg gttgctgatt gcttaatttg cggcttcctg
    77821 acgatgaaaa cctcgccacc agaccagtaa gttaaccagg gcaatcgtcg cgccagccag
    77881 acaaacgcca gcccaaccgc catgttgcta ggctgaggct gaaattagcg aaccggcggc
    77941 accgccaata aagtagctag tcatgtaacc tgcggtcagg cgattacgcg catcaggatg
    78001 tatccgataa attaccgtct ggttagtgat atgcacgccc tgcacggtga gatccagcac
    78061 caggattccg ataatcaacg ccagtacgga agtgtgacca aaccagatcg ccagccatga
    78121 aagtaatagc agcagcagac cgaaagttgt ggtgtggtgc gatttgccct tatcggcaaa
    78181 accgcccgcc ggacgagcgc ccaacgctcc ggcagctccc gcaagtccaa acagaccaat
    78241 gacaccatcg ctgtagttaa aaggtggagc ggcaagcaaa aaggccattg aggtccagag
    78301 aatgctgaag ttggcaaagg tcaggcagcc cagcaatgcg cgggtgcgca ggatcttatc
    78361 gctgataaac atgctgaaaa cggaacctaa caactgtggg tagttgaggt gggtttctga
    78421 tttcatttgt ggcagaccac gccataatgc cagcgccatc agtgccatta acaccgaggc
    78481 aacccaaaag acggtgcgcc agccgccgag attcgccagc aatccggcaa cggtccgtgc
    78541 gagtaagatc cccaacagca gaccgctcat aatagtgcca accactttgc cgcgtttgtc
    78601 cggtgaagcc agcgtcgctg ccagcggaac cagaatttgt gccacgacag agaataaacc
    78661 ggttaatgcc gtaccgagga tcatcatcgc cagcgactga ctgctggcag tgattaacat
    78721 gccgcctgcc gccagtaagg tcatcgagac aatcaggcgg cggcgttcaa acatatcacc
    78781 gaggggaaca agaaacagca gacctgctgc atagcccaat tgcgcggcgg taacaataaa
    78841 gcctgccgaa ctggcggaaa gggaaaagtt acgcgcgatg gtgtcgagca atggctgagc
    78901 gtaatagtta ctggcgaccg ccagaccggt ggcgatagac atcagcacga tcagcgccgg
    78961 gctaagctca tgattaggtt tagtcattat ttttgttcat caggtgatga ttaagctgga
    79021 aatcaataat agcggatgtt gatgaatatg ggatgaaaga tagcttgttg aaatgtgcga
    79081 tgtggtttgt aggcatgata agacgcgcca gcgtcgcatc aggcattgtg cacagaacgg
    79141 cggatgcggc gaaaacgcct tatccgccat acggaatgag tgtattttgt aggcatgata
    79201 agacgcgtca gcgtcgcatc aggcattgtg tacagagcgg cggatgaggc aaaaacgcct
    79261 tatccgcctg aataaaaatt acttctgcgc tgccagcgcc tcattcaccc agccatcgaa
    79321 ctgctgctgg tgggctttga tccagccatc aacgtggccc tgaatatcgc cttctgaggc
    79381 tttgccgtca tgcataatgg cgttctgggc gttaatatcg gccactggca actgcataat
    79441 ggcaaacagt ttggctgccg ccggattttt ctcggtccag gctttgttgg caacgatatg
    79501 catggtgctg accgggaagc cgtaattcgc gccattcggc agtttggtat cggcattttt
    79561 atcgcccggc agtgcggaga acggcacctg caaccagacc acatctttcc ctggcttcag
    79621 ctcattactc acccagtacg gcgtccaggt gtaataaaac accggtttgc cctctttgta
    79681 gcgactgatg gtgtcggcca tcatcgctgc gtagttcccc tgattatgcg tcacggtgtt
    79741 ggtcagttca tacgcggcaa gctggtggtt gatggcacct tcgcagcccc agccagggtt
    79801 acaaccggtt aaatccgctt taccgtcgcc gttggtatcg aacagtttgg cgatcttcgg
    79861 atctttcagt tgtgcgatgt tggtgatttt atactggtcg gcggttttct tatcgatcag
    79921 gtaaccctgt gccgcgccgt taacaaatac cccttcacga taaaatttct tatcgccacc
    79981 ggcagcttcg tacatgttgt catgcagtgg cgtccagttc acggcggtga aggttgcatc
    80041 gccggaagca agcgaggtgt agccaacgtt gtaatctact tcgctgggtt tgttgacggt
    80101 atagcctaat ttctccagcg cacggctgac cagcagcgtc tggaaggttt cttcggtgat
    80161 ggtgctttga actggattaa cagtaatacc tttgcccggc agatcggcag caaaagtttg
    80221 tgtagagata agcgtggcaa acgctgtcgc aaaaagtacg ctatgtcgca ttgttattcc
    80281 tttttttggc agggtgataa tgcccggcgt caccgggcaa gtgcagagtt acttaatgaa
    80341 tgggcgggtc agcagaccaa cagggccagt ggtgtaccag cgacggttgc cgcgactgcg
    80401 tgagtcgcgc ccaacggcct gcgtcaggcg atcgaggata atggcgagga tcacaatccc
    80461 gacgccgcca acggtggcaa gccccatatc cagacgaccg ataccgcgaa gtaccatctg
    80521 acccagcccg ccgacggcaa tcatcgaggc aatgaccacc atagaaaggg ccagcatcag
    80581 cgtctggtta acgcccgcca taatggtcgg catcgccagc ggtaactgaa ctttgaacag
    80641 catctggcgc gggctggcac cgaatgagcg cgaggcttca atcagatccg ccggaacctg
    80701 gttaatcccc agaatggtca gacggataat cggcggcaga gcaaagatga tcgtcaccac
    80761 cacgcccggc acgttaccga taccaaatag catgacgatt ggcaccagat aaacaaacgc
    80821 tggcgtggtc tgcatggcat caagcagtgg acgaataatt ttcgccgctc gcggacttct
    80881 cgccagccag atccccagcg gcaaaccaat gacgatacag aacagcaggg cggttaacac
    80941 cagcgccagg gtaaccatgg cctgcgacca ggcaccgatt gcgccgatgg cgatcagcga
    81001 aaccagcgtc gccacgccca ttccgacccc ggaaatctgc caggcgatga gagcgaaaac
    81061 gataatcgcc accggtgcgg gcatacccag cagcaattgc tggaaaccgt tgagaatgta
    81121 atcaaccgga acgcgcacgc cctggaagac gggacggaaa tgggtaacaa cccagtcgat
    81181 cccttcagtg acccaactgt cgagcgggat cagcgtttta tggaacggat cgagaatatt
    81241 aaaatgctcg acgtttggcg caggcgtact ggtcagccag tcagcaccac cgccgtcagt
    81301 cggtgcagtc gccggtgtac cccaggcgtc tgcggattgc acggcactgt ccgccgctgg
    81361 cgtggtatcc cacggattat tttgatcagc cattatttac cccctcacga tctaaagcgc
    81421 gcagcagcat tcctttcgaa atgatgccga catactgttg gtcctcgtcg accacgggca
    81481 ccgcacaggg tgcctgtccg acatgagaga gcaactcgct aagaggcgtt tgtgcatcga
    81541 ctgctaacgg cgcatcaatc agcgccgcat caagaccttg ctgctgcgtt aacgcggttt
    81601 taagcgaatc gatggaaact gcgccgacaa acttattacc gcgttcgata acgtagccat
    81661 attcgcgatc ttcatcctgc aataatttca gtgccgaacg tgggccgaag ccaggggttt
    81721 tacgaattaa gccattcggt gtccggcggg caatatcttt cgcactgaat acctgactaa
    81781 tatcaacgcc acggaagaag gtacggacat aatcattcgc cggattattg agaatttcat
    81841 ccggtgtgcc gacctgtacc acttcaccat tttgcataat ggcaattcgg tcgccaatac
    81901 gcatagcttc atcgagatcg tgggaaataa agacaatggt gcgctgatgt ttcgcctgta
    81961 attttaccag ctcatcctgc atctcggtgc gaattaatgg atcgagcgcc gagaaggctt
    82021 cgtccattaa taatatatcc ggattaatcg ctaacgcgcg ggctaatccc acacgttgac
    82081 gcatcccgcc agagagttca tccgggtagc tgtgggcata attttccagc ccgacctgac
    82141 gcagtgcatc aagggctttt tcccggcgtt cttcggcatt aattccggcc aattccatac
    82201 cgaacgcagt attgtccagc acggtcatat gcggcattaa ggcaaaggac tggaagacca
    82261 tcgcaatctt ttttctgcgc acctcacgga gttcggcatc ggatattttg gcaatatcca
    82321 caccatcaat cagcacttgc ccgcgggtgg gttcaatcag gcgattgaga aggcgtacca
    82381 ttgtggattt acccgagccg gataatccca tgatgacaaa tatctcgcct tcttcaatgg
    82441 ccagactggc gtcttttacg ccaagcgata gcccagtttt ttccagaatt tgttctttcg
    82501 aaagtccttg ttcgatatat ttgaacgctc gctgtggatg ctcgccaaat attttataaa
    82561 gatttttaat ttctaattta attgccatgc aatagaaaga ttcctttatt tgtttatgtc
    82621 gatatattac ccaatggaaa tagtcacttt tttctaccct aacatactga gaatctgagg
    82681 caacccctga gggcaaatgt ggcatgaata ttgatgcgag caaatcgccg gaatttcccg
    82741 tgatataagg gctgagagca aatcgagaaa agttgatatt ttttgtcata gatagcgaaa
    82801 aaacgcctga atattgtagt ttcaggcgaa taatgcaaca gagtctcagt gtagatcact
    82861 acagaatatt tgcgtgataa aaagctattt ggcgggaatt atttccctgc tgcgggtagt
    82921 gatatttttg aaaataacac cctcagaaat tccagtcttc atcttctgtt tcaaccgctt
    82981 tccccatcac ataagaggag cctgaaccgg aaaagaaatc gtgattttca tcggcattcg
    83041 gcgaaagcgc ggcgaggatt gccggattca cttccgccat ttctgcggga aataacggtt
    83101 cgtagcccag attcatcaaa gccttattgg cgttgtaaca gagaaacgct ttcacatcgt
    83161 cagcccacgg ggtttcggcg tacagctcat cggtgtattg caactcgttg tcgtagagtt
    83221 ccagcaacaa atcgaaggcg aaactcttca actcttcacg ttgtcccaga gatatctttt
    83281 ccatgttttt ctgatattta tagcctatgt agtaaccgtg gactgcttca tcgcggataa
    83341 tcagacggat cagatctgcg gtattggtca gctttccgcg gctggaaaaa tacatcggca
    83401 gccagaaacc ggaatagaac aaaaaagatt caagaaacac actggcgatt ttctttttca
    83461 gcggatcatc accgcgataa tgttgctgaa taatctgagc ttttcgctgc aacggtgcgt
    83521 tttcttcact ccaggcgtag gcggcatcga catctttggt ctgacacagc gtcgagaaaa
    83581 tcgaactgta agagcgggca tgaaccgctt ccataaagct gatattcgat aataccgctt
    83641 cttcatgagg cgtgagtgca tcgggcatca gcgaaggcgc gccgataaca ttttgcagcg
    83701 tgtcgagcag cgtcaggcca gtaaaaacgc gcatcgtcag ttgttgttct acgacagtta
    83761 atgtctgcca ggcaggaata tcgttcgaca gcggcacctt ttctggtagc cagaaattgc
    83821 tggtcaggcg attccacacc tccagatctt tatcgtcaga tatcttgttc cagttgatgg
    83881 cgctgatacg tgagagtttc atagatattc cttaaagtgc acaggagacg cagccttcaa
    83941 tttcagtgcc ttccagcgcc atctgacgca ggcgaatgta atagagcgtt ttgataccct
    84001 tgcgccaggc gtaaatctgc gctttgttga tatcgcgagt ggtggcggta tcggggaaaa
    84061 acagcgtcag cgacagcccc tgatcgacat ggcgagtcgc ttcggcgtag gtgtcgatga
    84121 tcttttctgc gccgatttcg taagcatcct gatacagcgc cagattctcg ttagtcataa
    84181 acggggcagg gtagtaaacg cgtcctgttt tgccctcttt gcgtatctct actttcgcca
    84241 caatcggatg aatactcgac gtagcatggt tgatataaga gatagaaccg gttggcggca
    84301 ccgcctgaag attctggtta taaatgccgt agcgcatcac gtcgtcgcgc agttgcgccc
    84361 acatctcacg ggtaggtaac gtaataccgc tgcgggcaaa cagttcgcca actttcgccg
    84421 ttttcggctg ccagttacct tgcagatatt ggctaaaata ttcaccactg gcataacgtg
    84481 actgtttgaa cccggcgaag gtttcaccgc gttcgcgcgc cagcaacatc gaggtacgca
    84541 gtgcgtgcca ggtgatggca tagaaataga gattggtgaa atccagtgct tccggcgaac
    84601 cataagcgat gccttctcgc gccagatagc cgtgtaaatt catctgcccc agtccgatgg
    84661 cgtgtgaggc ggcatttccg gcttcgatgg acggcacgct gcggatatga ctcatatctg
    84721 acaccgccgt taaaccgcgc acggcagtct ctaccgtgcg ggcaaaatcg ggggaatcca
    84781 tggtgtgcgc aatattcaac gaacctaaat tgcaggagat atcatggcct gtgcgggcat
    84841 agtcgagatt ctcgtcatac tctgaggcac agttgacctg caaaatctct gagcagagat
    84901 tactcatatt tatgcgcccg gcgacagggt tagcacggtt taccgtgtct tcatacatga
    84961 tgtagggata gccggactca aactggattt ctgccagtcg ctggaagaaa tcacgggcgt
    85021 tgaggtattt tttgcggatg cgttcatcgg caaccagttc gtcatagtgt tcgctaatgg
    85081 cgatatcggc aaacggcttg ccataaactc gctcaacgtc atagggcgaa aacagcgcca
    85141 tctgcgcatt ctctttggcc agatggaaag tgatatccgg gatcaccacg ccaagcgaca
    85201 gtgttttaat gcggattttt tcgtcggcat tttcccgttt cgtgtcgaga aaacgcagaa
    85261 tatcgggatg atgagcatgt aaatagactg caccagcccc ctgacgagcg ccgagttggt
    85321 tggcatagga aaatgcgtct tccagcattt tcatcaccgg aattacgcca gaagattgat
    85381 tttcaatacg tttaattggc gcgcccgctt cccgcagatt cgacagcaaa aatgccacgc
    85441 cgccgccgcg tttcgacagt tgcagtgcgg aatttaccgc ccgaccaatc gactccatat
    85501 tgtcttcaat acgcagcaaa aaacaggaaa ccagttcgcc gcgctgctgc ttaccgcagt
    85561 tgaggaatgt tggcgtggct ggctgaaagc gtcctgacag catttcatcg gtcagttgca
    85621 acgccagcgt ctcatcgcct tgtgccagcg tcagcgccac catcgtgact cgatcggcaa
    85681 aatcttccag ataacgttta ccgtcgaatg tcttcaacgt atagctggtg taaaacttcc
    85741 atgccccgag gaatgtatgg aaacgaaaac cgctggtgtg tgcgtgggca aacagcgtaa
    85801 tgacaaagtc gcgagagtag cgattaagaa cgctttcatc atagtaacct tcgttgacca
    85861 gccagttcag gcgctgctgc tggctactga aggtcacact gttcggacgc acatgcgtcg
    85921 caataaaggc gtcaacggcc tggcggtctt tatcgaactg aatgcgacct gcgctatcgt
    85981 agaggttaag catcgcattc agcgcgtggt aatccatcgt ttcctgcgtc aggcattctg
    86041 cggttgtcgt tgccaaaatt cggttactcc tttacgaacg ttttcgatat cgctttgcgt
    86101 acccatgagt tcaaaacggt acagccacgg cacgccgcat ttctgagcaa tcacatctcc
    86161 ggcacggcca tacgcctcac caaagttgcg attacccgaa gcaataacgc cgcgaagcaa
    86221 cgcccggttg tgctcgtcgt ttaaaaagcg aattacctgt cgtggcaccg cgccagccgt
    86281 accgccgccg ccgtaagagg gcacgatcag gatgtaaggc tcgtctacct gaatccgttc
    86341 ccgctcattg agcgggatgc gcaccgcggg cagacctaaa cgttcgataa aacgctgcgt
    86401 attttcggag ctgctggaga agtagacgag ctggctcatg cactggccgc gtgtggcgct
    86461 ggatgcagac ggttaatcat gtccggacgg aaaccagacc agctaagatc gccagcaatc
    86521 actaccggca actgacgaaa gccctgagca cgcaacgctt ctgccgcttc aggaacgcga
    86581 tcgacattaa tcatttcaaa atcaaagccc cggttttcca tcgcccgttt ggtggcgtgg
    86641 cactgaacgc aatcgttacg agtgtaaata gtaatgcgca tgattcgtat ttccgtttaa
    86701 aatgaagata cggcgcgatg atacgcgtcg ggttgtctca ctgttgatac agagatacta
    86761 gatgtagttg aaaaaagatt caaccacaca atatatagca aatagtggtc gaaattaccc
    86821 tggatatgag cgtgacgggg tagggggatt tttgtgattc accaggcaaa aagaaacccc
    86881 gaagacaggc ttcggggtca aagacgcgta tttattatca tttttgcact acgatttgcg
    86941 catgcttaac agtgcgccga taaaaatacc taccgcagct gctgtaccca cgctacacca
    87001 gggtctttca cgaacaaaag aatccgcgca gccaacggca tcgcgtgcgg cttgctggac
    87061 gcgagtacga ccatgcattc gcgccctggt ttctttcagc aatgcctgag ctttactgcg
    87121 tgcggcttca gcttcccctt tggcgtcgct gccccaggac tttaatacag attcgaggct
    87181 gtcagctaat tgattgacat cattctggat atcctgcacg ccatcatcaa cgtcgttgcg
    87241 gttcggtcgg ttaaacatat gatcccccat tagaattcga tgtaattttc agtgtagacc
    87301 ataattttct taactgaggt tcatcgcggc tttatggact tttctgaaag cgttgtgaat
    87361 gcgcaatcag gtaaggtagg cccgcggttg ataataacga ggatcaaata tgtatttacg
    87421 accagacgag gtggcgcgcg tacttgaaaa agtcggtttt actgtcgatg tggtaaccca
    87481 gaaagcgtat ggttaccgcc gtggggaaaa ttatgtctat gttaatcgcg aagcgcgaat
    87541 gggacgtacc gctctggtaa ttcatccaac attaaaagaa cgcagttcaa cactggctga
    87601 acccgcttcc gatattaaaa cttgcgatca ttaccagcaa tttccgctct atttagcagg
    87661 cgagcgacac gagcattacg gtatcccgca tggcttcagt tcgcgtgttg cgcttgaacg
    87721 ttatttgaat ggtttatttg gcgaagccag ttaaagacgc gactggcgat gccagtcgcg
    87781 aaaagaagag tcaggctttt acctgctggt aacggctgac tttaaacagt cggcggcaat
    87841 aatcgaggaa gtagccataa accgccccca tcaacatcga aacaacgatg tttgaactga
    87901 ccgccgccat aatctgatgc cagtctgcgc ccaccactaa caagatcgcc acatacaccg
    87961 gtgactggaa cgtcacataa gccaggatat cagccagatt ttttatccag cccgacgggc
    88021 taactttgcg tgccgcgcgc ataaacagat cgcggtacat accgtatggc catgcaatta
    88081 agatgttcac cggaatcgct accaatctgg aataaaaaga ctgttcgaag ctcattccgg
    88141 agaggaaaac ttcaatacac atgttcacga cagaacagta aacaaccatc gcgaacgtat
    88201 ctgcaactgc atgacgcaag cgtgactgcg gtgagaacat gctaatgctc cttaataaaa
    88261 gcgtaatggg atgttaatgg agatgaaagc agtggatctc attgccatct gcattttcgc
    88321 atagcgtata tcgctggcta tgactattca attagggaaa aatttttatt tagtcagttt
    88381 ttgtagataa aatattccgt aacggctcgt tttttgtaca ttgtggattt ttacatgccg
    88441 atatgattta ttttcaatta aatcaatcag atatgacctt gcctttaatt tcactaatga
    88501 aagtttgggg gtgaggtaac gctataagcg tagagaattc ggtgtatgtc gtgaccgtgt
    88561 catttacagc attttttatg ccagaaaatg tacattaaca gcagaaagca ccagtgaggg
    88621 gtgttttctt ttattctgtt atattgcatt tttattcact tcggttttgt caggcttgcg
    88681 gaattagcga gcagagagcg ccactctgtt caccaataat ctaaattact catcaaaatg
    88741 gctaatatat aatcttgaaa ttattctgag ag
//
LOCUS       DAZKHC020000006        87143 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZKHC020000006 DAZKHC020000000
VERSION     DAZKHC020000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 87143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 87143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.006"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..353)
                     /locus_tag="ACHOSM_RS02555"
                     /old_locus_tag="pgaptmp_000511"
     CDS             complement(<1..353)
                     /locus_tag="ACHOSM_RS02555"
                     /old_locus_tag="pgaptmp_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU4557097.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTR"
     gene            complement(424..2538)
                     /gene="fusA"
                     /locus_tag="ACHOSM_RS02560"
                     /old_locus_tag="pgaptmp_000512"
     CDS             complement(424..2538)
                     /gene="fusA"
                     /locus_tag="ACHOSM_RS02560"
                     /old_locus_tag="pgaptmp_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGU4557098.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2635..3105)
                     /gene="rpsG"
                     /locus_tag="ACHOSM_RS02565"
                     /old_locus_tag="pgaptmp_000513"
     CDS             complement(2635..3105)
                     /gene="rpsG"
                     /locus_tag="ACHOSM_RS02565"
                     /old_locus_tag="pgaptmp_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGU4557099.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3202..3576)
                     /gene="rpsL"
                     /locus_tag="ACHOSM_RS02570"
                     /old_locus_tag="pgaptmp_000514"
     CDS             complement(3202..3576)
                     /gene="rpsL"
                     /locus_tag="ACHOSM_RS02570"
                     /old_locus_tag="pgaptmp_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGU4557100.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3702..3989)
                     /gene="tusB"
                     /locus_tag="ACHOSM_RS02575"
                     /old_locus_tag="pgaptmp_000515"
     CDS             complement(3702..3989)
                     /gene="tusB"
                     /locus_tag="ACHOSM_RS02575"
                     /old_locus_tag="pgaptmp_000515"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HGU4557101.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3997..4356)
                     /gene="tusC"
                     /locus_tag="ACHOSM_RS02580"
                     /old_locus_tag="pgaptmp_000516"
     CDS             complement(3997..4356)
                     /gene="tusC"
                     /locus_tag="ACHOSM_RS02580"
                     /old_locus_tag="pgaptmp_000516"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HGU4557102.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4356..4742)
                     /gene="tusD"
                     /locus_tag="ACHOSM_RS02585"
                     /old_locus_tag="pgaptmp_000517"
     CDS             complement(4356..4742)
                     /gene="tusD"
                     /locus_tag="ACHOSM_RS02585"
                     /old_locus_tag="pgaptmp_000517"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HGU4557103.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4742..5464)
                     /gene="yheO"
                     /locus_tag="ACHOSM_RS02590"
                     /old_locus_tag="pgaptmp_000518"
     CDS             complement(4742..5464)
                     /gene="yheO"
                     /locus_tag="ACHOSM_RS02590"
                     /old_locus_tag="pgaptmp_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4557104.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5631..6443)
                     /gene="fkpA"
                     /locus_tag="ACHOSM_RS02595"
                     /old_locus_tag="pgaptmp_000519"
     CDS             complement(5631..6443)
                     /gene="fkpA"
                     /locus_tag="ACHOSM_RS02595"
                     /old_locus_tag="pgaptmp_000519"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4557105.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6664..6882
                     /gene="slyX"
                     /locus_tag="ACHOSM_RS02600"
                     /old_locus_tag="pgaptmp_000520"
     CDS             6664..6882
                     /gene="slyX"
                     /locus_tag="ACHOSM_RS02600"
                     /old_locus_tag="pgaptmp_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HGU4557106.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6931..7521)
                     /gene="slyD"
                     /locus_tag="ACHOSM_RS02605"
                     /old_locus_tag="pgaptmp_000521"
     CDS             complement(6931..7521)
                     /gene="slyD"
                     /locus_tag="ACHOSM_RS02605"
                     /old_locus_tag="pgaptmp_000521"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU4557107.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7616..7816)
                     /gene="yheV"
                     /locus_tag="ACHOSM_RS02610"
                     /old_locus_tag="pgaptmp_000522"
     CDS             complement(7616..7816)
                     /gene="yheV"
                     /locus_tag="ACHOSM_RS02610"
                     /old_locus_tag="pgaptmp_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HGU4557108.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7826..9631)
                     /gene="kefB"
                     /locus_tag="ACHOSM_RS02615"
                     /old_locus_tag="pgaptmp_000523"
     CDS             complement(7826..9631)
                     /gene="kefB"
                     /locus_tag="ACHOSM_RS02615"
                     /old_locus_tag="pgaptmp_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HGU4557109.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9631..10182)
                     /gene="kefG"
                     /locus_tag="ACHOSM_RS02620"
                     /old_locus_tag="pgaptmp_000524"
     CDS             complement(9631..10182)
                     /gene="kefG"
                     /locus_tag="ACHOSM_RS02620"
                     /old_locus_tag="pgaptmp_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HGU4557110.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10313..12226
                     /gene="yheS"
                     /locus_tag="ACHOSM_RS02625"
                     /old_locus_tag="pgaptmp_000525"
     CDS             10313..12226
                     /gene="yheS"
                     /locus_tag="ACHOSM_RS02625"
                     /old_locus_tag="pgaptmp_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4557111.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSKLYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12226..13248
                     /gene="yheT"
                     /locus_tag="ACHOSM_RS02630"
                     /old_locus_tag="pgaptmp_000526"
     CDS             12226..13248
                     /gene="yheT"
                     /locus_tag="ACHOSM_RS02630"
                     /old_locus_tag="pgaptmp_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU4557112.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13242..13460
                     /gene="yheU"
                     /locus_tag="ACHOSM_RS02635"
                     /old_locus_tag="pgaptmp_000527"
     CDS             13242..13460
                     /gene="yheU"
                     /locus_tag="ACHOSM_RS02635"
                     /old_locus_tag="pgaptmp_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HGU4557113.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13514..14383
                     /gene="prkB"
                     /locus_tag="ACHOSM_RS02640"
                     /old_locus_tag="pgaptmp_000528"
     CDS             13514..14383
                     /gene="prkB"
                     /locus_tag="ACHOSM_RS02640"
                     /old_locus_tag="pgaptmp_000528"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HGU4557114.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14438..14842)
                     /gene="yhfA"
                     /locus_tag="ACHOSM_RS02645"
                     /old_locus_tag="pgaptmp_000529"
     CDS             complement(14438..14842)
                     /gene="yhfA"
                     /locus_tag="ACHOSM_RS02645"
                     /old_locus_tag="pgaptmp_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HGU4557115.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15144..15776
                     /gene="crp"
                     /locus_tag="ACHOSM_RS02650"
                     /old_locus_tag="pgaptmp_000530"
     CDS             15144..15776
                     /gene="crp"
                     /locus_tag="ACHOSM_RS02650"
                     /old_locus_tag="pgaptmp_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HGU4557116.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15827..17917
                     /gene="yhfK"
                     /locus_tag="ACHOSM_RS02655"
                     /old_locus_tag="pgaptmp_000531"
     CDS             15827..17917
                     /gene="yhfK"
                     /locus_tag="ACHOSM_RS02655"
                     /old_locus_tag="pgaptmp_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HGU4557117.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEEPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17983..19203)
                     /gene="argD"
                     /locus_tag="ACHOSM_RS02660"
                     /old_locus_tag="pgaptmp_000532"
                     /gene_synonym="dapC"
     CDS             complement(17983..19203)
                     /gene="argD"
                     /locus_tag="ACHOSM_RS02660"
                     /old_locus_tag="pgaptmp_000532"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HGU4557118.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVIGA"
     gene            complement(19289..19852)
                     /gene="pabA"
                     /locus_tag="ACHOSM_RS02665"
                     /old_locus_tag="pgaptmp_000533"
     CDS             complement(19289..19852)
                     /gene="pabA"
                     /locus_tag="ACHOSM_RS02665"
                     /old_locus_tag="pgaptmp_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HGU4557119.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19884..20486)
                     /gene="fic"
                     /locus_tag="ACHOSM_RS02670"
                     /old_locus_tag="pgaptmp_000534"
     CDS             complement(19884..20486)
                     /gene="fic"
                     /locus_tag="ACHOSM_RS02670"
                     /old_locus_tag="pgaptmp_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HGU4557120.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20476..20643)
                     /gene="yhfG"
                     /locus_tag="ACHOSM_RS02675"
                     /old_locus_tag="pgaptmp_000535"
     CDS             complement(20476..20643)
                     /gene="yhfG"
                     /locus_tag="ACHOSM_RS02675"
                     /old_locus_tag="pgaptmp_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HGU4557121.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLVELRRHYER"
     gene            complement(20748..21320)
                     /gene="ppiA"
                     /locus_tag="ACHOSM_RS02680"
                     /old_locus_tag="pgaptmp_000536"
                     /gene_synonym="rotA"
     CDS             complement(20748..21320)
                     /gene="ppiA"
                     /locus_tag="ACHOSM_RS02680"
                     /old_locus_tag="pgaptmp_000536"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HGU4557122.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21591..22772
                     /gene="tsgA"
                     /locus_tag="ACHOSM_RS02685"
                     /old_locus_tag="pgaptmp_000537"
     CDS             21591..22772
                     /gene="tsgA"
                     /locus_tag="ACHOSM_RS02685"
                     /old_locus_tag="pgaptmp_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HGU4557123.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23034..25577
                     /gene="nirB"
                     /locus_tag="ACHOSM_RS02690"
                     /old_locus_tag="pgaptmp_000538"
     CDS             23034..25577
                     /gene="nirB"
                     /locus_tag="ACHOSM_RS02690"
                     /old_locus_tag="pgaptmp_000538"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HGU4557124.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVYKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25574..25900
                     /gene="nirD"
                     /locus_tag="ACHOSM_RS02695"
                     /old_locus_tag="pgaptmp_000539"
     CDS             25574..25900
                     /gene="nirD"
                     /locus_tag="ACHOSM_RS02695"
                     /old_locus_tag="pgaptmp_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HGU4557125.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26027..26833
                     /gene="nirC"
                     /locus_tag="ACHOSM_RS02700"
                     /old_locus_tag="pgaptmp_000540"
     CDS             26027..26833
                     /gene="nirC"
                     /locus_tag="ACHOSM_RS02700"
                     /old_locus_tag="pgaptmp_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HGU4557126.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26852..28225
                     /gene="cysG"
                     /locus_tag="ACHOSM_RS02705"
                     /old_locus_tag="pgaptmp_000541"
     CDS             26852..28225
                     /gene="cysG"
                     /locus_tag="ACHOSM_RS02705"
                     /old_locus_tag="pgaptmp_000541"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HGU4557127.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFTTMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28473..28640
                     /gene="yhfL"
                     /locus_tag="ACHOSM_RS02710"
                     /old_locus_tag="pgaptmp_000542"
     CDS             28473..28640
                     /gene="yhfL"
                     /locus_tag="ACHOSM_RS02710"
                     /old_locus_tag="pgaptmp_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HGU4557128.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28936..30273
                     /gene="frlA"
                     /locus_tag="ACHOSM_RS02715"
                     /old_locus_tag="pgaptmp_000543"
     CDS             28936..30273
                     /gene="frlA"
                     /locus_tag="ACHOSM_RS02715"
                     /old_locus_tag="pgaptmp_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HGU4557129.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLPGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTSTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30294..31316
                     /gene="frlB"
                     /locus_tag="ACHOSM_RS02720"
                     /old_locus_tag="pgaptmp_000544"
     CDS             30294..31316
                     /gene="frlB"
                     /locus_tag="ACHOSM_RS02720"
                     /old_locus_tag="pgaptmp_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HGU4557130.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31366..32196
                     /gene="frlC"
                     /locus_tag="ACHOSM_RS02725"
                     /old_locus_tag="pgaptmp_000545"
     CDS             31366..32196
                     /gene="frlC"
                     /locus_tag="ACHOSM_RS02725"
                     /old_locus_tag="pgaptmp_000545"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HGU4557131.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLMPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32193..32978
                     /gene="frlD"
                     /locus_tag="ACHOSM_RS02730"
                     /old_locus_tag="pgaptmp_000546"
     CDS             32193..32978
                     /gene="frlD"
                     /locus_tag="ACHOSM_RS02730"
                     /old_locus_tag="pgaptmp_000546"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HGU4557132.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33078..33809
                     /gene="frlR"
                     /locus_tag="ACHOSM_RS02735"
                     /old_locus_tag="pgaptmp_000547"
                     /gene_synonym="yhfR"
     CDS             33078..33809
                     /gene="frlR"
                     /locus_tag="ACHOSM_RS02735"
                     /old_locus_tag="pgaptmp_000547"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HGU4557133.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33961..35046)
                     /gene="yhfS"
                     /locus_tag="ACHOSM_RS02740"
                     /old_locus_tag="pgaptmp_000548"
     CDS             complement(33961..35046)
                     /gene="yhfS"
                     /locus_tag="ACHOSM_RS02740"
                     /old_locus_tag="pgaptmp_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGU4557134.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVIHRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35058..36362)
                     /gene="yhfT"
                     /locus_tag="ACHOSM_RS02745"
                     /old_locus_tag="pgaptmp_000549"
     CDS             complement(35058..36362)
                     /gene="yhfT"
                     /locus_tag="ACHOSM_RS02745"
                     /old_locus_tag="pgaptmp_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HGU4557135.1"
                     /translation="MDLYIQIIVVACLAGMTSLLAHRSAAVFHDGIRPLLPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMASYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36374..36727)
                     /gene="yhfU"
                     /locus_tag="ACHOSM_RS02750"
                     /old_locus_tag="pgaptmp_000550"
     CDS             complement(36374..36727)
                     /gene="yhfU"
                     /locus_tag="ACHOSM_RS02750"
                     /old_locus_tag="pgaptmp_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HGU4557136.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36738..37616)
                     /gene="php"
                     /locus_tag="ACHOSM_RS02755"
                     /old_locus_tag="pgaptmp_000551"
     CDS             complement(36738..37616)
                     /gene="php"
                     /locus_tag="ACHOSM_RS02755"
                     /old_locus_tag="pgaptmp_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HGU4557137.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(37613..38839)
                     /gene="yhfW"
                     /locus_tag="ACHOSM_RS02760"
                     /old_locus_tag="pgaptmp_000552"
                     /pseudo
     CDS             complement(37613..38839)
                     /gene="yhfW"
                     /locus_tag="ACHOSM_RS02760"
                     /old_locus_tag="pgaptmp_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            complement(38839..40002)
                     /gene="yhfX"
                     /locus_tag="ACHOSM_RS02765"
                     /old_locus_tag="pgaptmp_000553"
     CDS             complement(38839..40002)
                     /gene="yhfX"
                     /locus_tag="ACHOSM_RS02765"
                     /old_locus_tag="pgaptmp_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HGU4557138.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(40086..40448)
                     /gene="yhfY"
                     /locus_tag="ACHOSM_RS02770"
                     /old_locus_tag="pgaptmp_000554"
     CDS             complement(40086..40448)
                     /gene="yhfY"
                     /locus_tag="ACHOSM_RS02770"
                     /old_locus_tag="pgaptmp_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGU4557139.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40465..41370)
                     /gene="yhfZ"
                     /locus_tag="ACHOSM_RS02775"
                     /old_locus_tag="pgaptmp_000555"
     CDS             complement(40465..41370)
                     /gene="yhfZ"
                     /locus_tag="ACHOSM_RS02775"
                     /old_locus_tag="pgaptmp_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HGU4557140.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQTALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41639..42643)
                     /gene="trpS"
                     /locus_tag="ACHOSM_RS02780"
                     /old_locus_tag="pgaptmp_000556"
     CDS             complement(41639..42643)
                     /gene="trpS"
                     /locus_tag="ACHOSM_RS02780"
                     /old_locus_tag="pgaptmp_000556"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGU4557141.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42636..43394)
                     /gene="gph"
                     /locus_tag="ACHOSM_RS02785"
                     /old_locus_tag="pgaptmp_000557"
     CDS             complement(42636..43394)
                     /gene="gph"
                     /locus_tag="ACHOSM_RS02785"
                     /old_locus_tag="pgaptmp_000557"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HGU4557142.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDCYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43387..44064)
                     /gene="rpe"
                     /locus_tag="ACHOSM_RS02790"
                     /old_locus_tag="pgaptmp_000558"
     CDS             complement(43387..44064)
                     /gene="rpe"
                     /locus_tag="ACHOSM_RS02790"
                     /old_locus_tag="pgaptmp_000558"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGU4557143.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44082..44918)
                     /gene="dam"
                     /locus_tag="ACHOSM_RS02795"
                     /old_locus_tag="pgaptmp_000559"
     CDS             complement(44082..44918)
                     /gene="dam"
                     /locus_tag="ACHOSM_RS02795"
                     /old_locus_tag="pgaptmp_000559"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU4557144.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(45025..46311)
                     /gene="damX"
                     /locus_tag="ACHOSM_RS02800"
                     /old_locus_tag="pgaptmp_000560"
     CDS             complement(45025..46311)
                     /gene="damX"
                     /locus_tag="ACHOSM_RS02800"
                     /old_locus_tag="pgaptmp_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HGU4557145.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46403..47491)
                     /gene="aroB"
                     /locus_tag="ACHOSM_RS02805"
                     /old_locus_tag="pgaptmp_000561"
     CDS             complement(46403..47491)
                     /gene="aroB"
                     /locus_tag="ACHOSM_RS02805"
                     /old_locus_tag="pgaptmp_000561"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGU4557146.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47548..48069)
                     /gene="aroK"
                     /locus_tag="ACHOSM_RS02810"
                     /old_locus_tag="pgaptmp_000562"
     CDS             complement(47548..48069)
                     /gene="aroK"
                     /locus_tag="ACHOSM_RS02810"
                     /old_locus_tag="pgaptmp_000562"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HGU4557147.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48470..49708)
                     /gene="hofQ"
                     /locus_tag="ACHOSM_RS02815"
                     /old_locus_tag="pgaptmp_000563"
     CDS             complement(48470..49708)
                     /gene="hofQ"
                     /locus_tag="ACHOSM_RS02815"
                     /old_locus_tag="pgaptmp_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HGU4557148.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49620..50024)
                     /gene="hofP"
                     /locus_tag="ACHOSM_RS02820"
                     /old_locus_tag="pgaptmp_000564"
     CDS             complement(49620..50024)
                     /gene="hofP"
                     /locus_tag="ACHOSM_RS02820"
                     /old_locus_tag="pgaptmp_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HGU4557149.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(50014..50446)
                     /gene="hofO"
                     /locus_tag="ACHOSM_RS02825"
                     /old_locus_tag="pgaptmp_000565"
                     /pseudo
     CDS             complement(50014..50446)
                     /gene="hofO"
                     /locus_tag="ACHOSM_RS02825"
                     /old_locus_tag="pgaptmp_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
     gene            complement(50430..50969)
                     /gene="hofN"
                     /locus_tag="ACHOSM_RS02830"
                     /old_locus_tag="pgaptmp_000566"
     CDS             complement(50430..50969)
                     /gene="hofN"
                     /locus_tag="ACHOSM_RS02830"
                     /old_locus_tag="pgaptmp_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HGU4557150.1"
                     /translation="MNPSINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRQTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50969..51748)
                     /gene="hofM"
                     /locus_tag="ACHOSM_RS02835"
                     /old_locus_tag="pgaptmp_000567"
     CDS             complement(50969..51748)
                     /gene="hofM"
                     /locus_tag="ACHOSM_RS02835"
                     /old_locus_tag="pgaptmp_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HGU4557151.1"
                     /translation="MAFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51868..54420
                     /gene="mrcA"
                     /locus_tag="ACHOSM_RS02840"
                     /old_locus_tag="pgaptmp_000568"
     CDS             51868..54420
                     /gene="mrcA"
                     /locus_tag="ACHOSM_RS02840"
                     /old_locus_tag="pgaptmp_000568"
                     /EC_number="2.4.99.28"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HGU4557152.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54585..55145)
                     /gene="nudE"
                     /locus_tag="ACHOSM_RS02845"
                     /old_locus_tag="pgaptmp_000569"
     CDS             complement(54585..55145)
                     /gene="nudE"
                     /locus_tag="ACHOSM_RS02845"
                     /old_locus_tag="pgaptmp_000569"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HGU4557153.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55465..57600
                     /gene="igaA"
                     /locus_tag="ACHOSM_RS02850"
                     /old_locus_tag="pgaptmp_000570"
     CDS             55465..57600
                     /gene="igaA"
                     /locus_tag="ACHOSM_RS02850"
                     /old_locus_tag="pgaptmp_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HGU4557154.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLSWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57665..58333
                     /gene="yrfG"
                     /locus_tag="ACHOSM_RS02855"
                     /old_locus_tag="pgaptmp_000571"
     CDS             57665..58333
                     /gene="yrfG"
                     /locus_tag="ACHOSM_RS02855"
                     /old_locus_tag="pgaptmp_000571"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HGU4557155.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58344..58745
                     /gene="hslR"
                     /locus_tag="ACHOSM_RS02860"
                     /old_locus_tag="pgaptmp_000572"
     CDS             58344..58745
                     /gene="hslR"
                     /locus_tag="ACHOSM_RS02860"
                     /old_locus_tag="pgaptmp_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HGU4557156.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58770..59648
                     /gene="hslO"
                     /locus_tag="ACHOSM_RS02865"
                     /old_locus_tag="pgaptmp_000573"
     CDS             58770..59648
                     /gene="hslO"
                     /locus_tag="ACHOSM_RS02865"
                     /old_locus_tag="pgaptmp_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGU4557157.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59783..61507)
                     /gene="yhgE"
                     /locus_tag="ACHOSM_RS02870"
                     /old_locus_tag="pgaptmp_000574"
     CDS             complement(59783..61507)
                     /gene="yhgE"
                     /locus_tag="ACHOSM_RS02870"
                     /old_locus_tag="pgaptmp_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HGU4557158.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     LGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDLVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61886..63508
                     /gene="pckA"
                     /locus_tag="ACHOSM_RS02875"
                     /old_locus_tag="pgaptmp_000575"
     CDS             61886..63508
                     /gene="pckA"
                     /locus_tag="ACHOSM_RS02875"
                     /old_locus_tag="pgaptmp_000575"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HGU4557159.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGISSMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63584..64936)
                     /gene="envZ"
                     /locus_tag="ACHOSM_RS02880"
                     /old_locus_tag="pgaptmp_000576"
     CDS             complement(63584..64936)
                     /gene="envZ"
                     /locus_tag="ACHOSM_RS02880"
                     /old_locus_tag="pgaptmp_000576"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HGU4557160.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64933..65652)
                     /gene="ompR"
                     /locus_tag="ACHOSM_RS02885"
                     /old_locus_tag="pgaptmp_000577"
     CDS             complement(64933..65652)
                     /gene="ompR"
                     /locus_tag="ACHOSM_RS02885"
                     /old_locus_tag="pgaptmp_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HGU4557161.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65880..66356
                     /gene="greB"
                     /locus_tag="ACHOSM_RS02890"
                     /old_locus_tag="pgaptmp_000578"
     CDS             65880..66356
                     /gene="greB"
                     /locus_tag="ACHOSM_RS02890"
                     /old_locus_tag="pgaptmp_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HGU4557162.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            66453..68774
                     /gene="yhgF"
                     /locus_tag="ACHOSM_RS02895"
                     /old_locus_tag="pgaptmp_000579"
     CDS             66453..68774
                     /gene="yhgF"
                     /locus_tag="ACHOSM_RS02895"
                     /old_locus_tag="pgaptmp_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGU4557163.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHESLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69212..69439
                     /gene="feoA"
                     /locus_tag="ACHOSM_RS02900"
                     /old_locus_tag="pgaptmp_000580"
     CDS             69212..69439
                     /gene="feoA"
                     /locus_tag="ACHOSM_RS02900"
                     /old_locus_tag="pgaptmp_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HGU4557164.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69456..71777
                     /gene="feoB"
                     /locus_tag="ACHOSM_RS02905"
                     /old_locus_tag="pgaptmp_000581"
     CDS             69456..71777
                     /gene="feoB"
                     /locus_tag="ACHOSM_RS02905"
                     /old_locus_tag="pgaptmp_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HGU4557165.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71777..72013
                     /gene="feoC"
                     /locus_tag="ACHOSM_RS02910"
                     /old_locus_tag="pgaptmp_000582"
     CDS             71777..72013
                     /gene="feoC"
                     /locus_tag="ACHOSM_RS02910"
                     /old_locus_tag="pgaptmp_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HGU4557166.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72216..73094
                     /gene="rpnA"
                     /locus_tag="ACHOSM_RS02915"
                     /old_locus_tag="pgaptmp_000583"
     CDS             72216..73094
                     /gene="rpnA"
                     /locus_tag="ACHOSM_RS02915"
                     /old_locus_tag="pgaptmp_000583"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HGU4557167.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRIALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(73123..73893)
                     /gene="bioH"
                     /locus_tag="ACHOSM_RS02920"
                     /old_locus_tag="pgaptmp_000584"
     CDS             complement(73123..73893)
                     /gene="bioH"
                     /locus_tag="ACHOSM_RS02920"
                     /old_locus_tag="pgaptmp_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HGU4557168.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWCCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            73931..74614
                     /gene="gntX"
                     /locus_tag="ACHOSM_RS02925"
                     /old_locus_tag="pgaptmp_000585"
     CDS             73931..74614
                     /gene="gntX"
                     /locus_tag="ACHOSM_RS02925"
                     /old_locus_tag="pgaptmp_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HGU4557169.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            74673..75248
                     /gene="nfuA"
                     /locus_tag="ACHOSM_RS02930"
                     /old_locus_tag="pgaptmp_000586"
     CDS             74673..75248
                     /gene="nfuA"
                     /locus_tag="ACHOSM_RS02930"
                     /old_locus_tag="pgaptmp_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HGU4557170.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            75609..76925
                     /gene="gntT"
                     /locus_tag="ACHOSM_RS02935"
                     /old_locus_tag="pgaptmp_000587"
     CDS             75609..76925
                     /gene="gntT"
                     /locus_tag="ACHOSM_RS02935"
                     /old_locus_tag="pgaptmp_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU4557171.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77036..79120)
                     /gene="malQ"
                     /locus_tag="ACHOSM_RS02940"
                     /old_locus_tag="pgaptmp_000588"
     CDS             complement(77036..79120)
                     /gene="malQ"
                     /locus_tag="ACHOSM_RS02940"
                     /old_locus_tag="pgaptmp_000588"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HGU4557172.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79130..81523)
                     /gene="malP"
                     /locus_tag="ACHOSM_RS02945"
                     /old_locus_tag="pgaptmp_000589"
     CDS             complement(79130..81523)
                     /gene="malP"
                     /locus_tag="ACHOSM_RS02945"
                     /old_locus_tag="pgaptmp_000589"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HGU4557173.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            82135..84840
                     /gene="malT"
                     /locus_tag="ACHOSM_RS02950"
                     /old_locus_tag="pgaptmp_000590"
     CDS             82135..84840
                     /gene="malT"
                     /locus_tag="ACHOSM_RS02950"
                     /old_locus_tag="pgaptmp_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HGU4557174.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSQLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGEYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(84883..85899)
                     /gene="rtcA"
                     /locus_tag="ACHOSM_RS02955"
                     /old_locus_tag="pgaptmp_000591"
     CDS             complement(84883..85899)
                     /gene="rtcA"
                     /locus_tag="ACHOSM_RS02955"
                     /old_locus_tag="pgaptmp_000591"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HGU4557175.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     SGLLRQHLTAVKAAAEICRATVEGAELGSQRLLFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(85903..87129)
                     /gene="rtcB"
                     /locus_tag="ACHOSM_RS02960"
                     /old_locus_tag="pgaptmp_000592"
     CDS             complement(85903..87129)
                     /gene="rtcB"
                     /locus_tag="ACHOSM_RS02960"
                     /old_locus_tag="pgaptmp_000592"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HGU4557176.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     RTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        20796 a        23785 c        22022 g        20540 t
ORIGIN      
        1 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
       61 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgt
      121 gcgtagtgac gggtcggggt gtcatattca acgtgagaag tgttgatggt gataccacga
      181 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcac gagcagcacc gccgtaggtt
      241 ttagccagta cggtggtgat tgcagcggtc agagtagttt taccgtggtc aacgtggccg
      301 atagtaccaa cgttaacgtg cggttttgta cgttcaaatt tttctttaga cacggctata
      361 ttccttacta tagtgctctc cccttcagga gagagcacgg gactttggta ttaaccctta
      421 ggcttattta ccacgggctt caattacggc ctgagcaacg ttactcggcg cttcatcata
      481 cttcaggaat tccatagtgt atgatgcacg acctttggtc agagaacgca gctgagttgc
      541 gtatccgaac atttcagaca gcggtacttc agcgtggatc ttaacgccag taacttcaga
      601 ttcctgacct ttgagcatac cacgacgacg gctcaagtca ccgataacgt caccggtgtt
      661 ctcttccgga gtttctactt caaccttcat gatcggctca agcagaactg gtttcgcttt
      721 cttaaagcct tctttaaagg cgatagaagc agccagttta aacgccagtt cagaggagtc
      781 aacgtcatgg taagaaccga agtgcagacg aatacccatg tctactaccg ggtagcctgc
      841 cagcggacct gctttcagct gttcctggat acctttatca acggccggga tgtattcgcc
      901 agggattaca ccacctttaa tgtcgttgat gaactcgtag cctttcgggt ttgaacccgg
      961 ctccagcggg tacatgtcga taacaacatg accatactga ccacgaccac cagactgttt
     1021 cgcgtgttta ccttcaacat cggtaacttt ctggcggata gtttcacggt aagcaacctg
     1081 cggtttacct acgttcgctt caacgttgaa ttcacgcttc atacggtcaa cgatgatgtc
     1141 gaggtgcagt tcgcccatac ccgcgatgat ggtctggtta gattcttcgt cagtccatac
     1201 acggaaagac gggtcttctt tagccagacg gcccagagcc agacccattt tttcctggtc
     1261 agctttggtt ttcggttcaa ctgcgatgga gattaccggc tcagggaatt ccatacgttc
     1321 cagaatgatc ggcgcatccg ggtcacacag ggtgtcacca gtggttacgt ctttcagacc
     1381 gatagcagca gcgatgtcgc ccgcgcgaac ttctttgatc tcttcacgtt tgttagcgtg
     1441 catctgaacg atacgaccga aacgctcacg tgcagctttc acggagttca gtacggtatc
     1501 accagagtta accacaccgg agtaaacacg gaagaaggtc aggttaccaa caaacgggtc
     1561 ggtagcgatt ttgaacgcca gtgcagagaa cggctcgtca tcacttgcgt gacgttcagc
     1621 cggagtgtct ttaccgtcgt ccaggatacc gttgatcgca ggtacgtcaa ccggggatgg
     1681 caggtaatca attaccgcat ccagcatcgc ctgaacacct ttgttcttga acgcagaacc
     1741 acaggttacc aggatgattt cgttgttcag aacgcgctga cgcagagcac ctttgatttc
     1801 tgcttcagtc agttcttcac cacccaggta tttttccatc agctcttcag aagcttcagc
     1861 tgcggattcg atcaggttct ggtgccattc gttagccagt tcaaccatgt ctgccgggat
     1921 atcttcgtat tcgaaggtta cgccctggtc agcgtcgttc cagttgatag ctttcatttt
     1981 caccaggtca acaacaccgg tgaaatgttc ttcagcacca atcgccagct gcagcggaac
     2041 cgggttcgcg cccagacggg ttttgatctg gttaacaact ttcaggaagt tcgcacccat
     2101 gcggtccatt ttgttaacga acgcaatgcg cggaacttta tatttgtttg cctgacgcca
     2161 tacggtttca gactgcggct gaacaccacc aactgcgcag taaaccatta ccgcaccatc
     2221 gagaacacgc atggaacgtt ctacttcgat tgtgaagtca acgtgccccg gggtgtcgat
     2281 gatgttgatg cgatgcggct catactgctt agccatacca gaccagaatg cagtagtcgc
     2341 agcggaagtg atggtaatac cacgttcctg ctcctgctcc atccagtcca tggttgcagc
     2401 gccgtcatga acttcaccga ttttatggtt tacaccggtg tagaacagaa tacgttcggt
     2461 agtagtggtt ttaccggcgt cgatgtgcgc actgataccg atgttacggt agcgtgcgat
     2521 gggtgttgta cgagccattt gtttcctcgt ttatctttta ggcgttcaat ttaagtagcc
     2581 caaagcgggc tgcttactgg aagcgcccgc ctggtgacta aaactccgaa gggattacca
     2641 acggtagtgt gcgaacgcct tgttggcttc ggccatacgg tgaacgtctt cacgtttctt
     2701 aactgcagta cctttgtttt ctgcagcatc agaaagttcg ttcgccaggc gcagagccat
     2761 ggatttatca ccgcgtttac gagcagcttc aacgatccaa cgcattgcca gagcattacg
     2821 acgaaccgga cggacttcaa ctggtacctg ataagtagaa ccaccaacgc ggcgagactt
     2881 aacttctaca gtcgggcgca cgttttcgag agctacttcg aatgcttcca gttcagattt
     2941 accagagcgc tgagccaggg tctccagcgc gctgtatacg atagattcag cagtagattt
     3001 tttaccatct accatcagga tatttacaaa tttagccagc agttctgatc cgaacttcgg
     3061 atccggcaga attttacgct gaccaatgac gcgacgacgt ggcatggaaa tactccgttg
     3121 ttaattcagg attgtccaaa actctacgag tttagtttga catttaagtt aaaacgtttg
     3181 gccttactta acggagaacc attaagcctt aggacgcttc acgccatact tggaacgagc
     3241 ctgcttacgg tctttaacgc cggagcagtc aagcgcacca cgtacggtgt ggtaacgaac
     3301 acccgggagg tctttaacac gaccgccacg gatcaggatc acggagtgct cctgcaggtt
     3361 gtgaccttca ccaccgatgt aggaagtcac ttcgaaaccg ttagtcagac gaacacggca
     3421 tactttacgc agcgcggagt tcggtttttt aggagtggta gtatatacac gagtacatac
     3481 gccacgtttt tgcgggcatg cttccagcgc aggcacgttg cttttcgcaa ctttgcgagc
     3541 acgtggtttg cgtaccagct ggttaactgt tgccattaaa tagctcctgg ttttagcttt
     3601 tgcttcgtaa acacgtaata aaacgtcctc acacaatatg aggacgccga attttagggc
     3661 gatgccgaaa aggtgtcaag aaatatacaa cgatcccgcc atcaccaggc catctggctg
     3721 gagtgcttaa ccgtaagtct gacgaaatca gtatagtcaa tgggaatgat gtcgttcgaa
     3781 atttgaccag tcaaaccgcg ggcaataagg tcttcgttca gggcatagac cttaatgggg
     3841 gcattacgca gactttcaag gtagcggtta ccgtcaactg cggcagttac gccatcttgc
     3901 aataatagca gttcgtctcc ttcactgagc agacgcagca gcgcagcaaa atccgtcagc
     3961 cagggtgaac gatgtaacgt gtgcagcata aacgcctcaa aacctcaaaa taacatcgta
     4021 gttggcgagt tcgcggcgta aggcatctgc ttcgagcggc gtggcttcga caacaaaggg
     4081 tgtctgcgga tctaacccgc gttcgcgcag tgaagccgca caaacccagc actgttcaat
     4141 gtcgtacaga cccaacaatt taaaagtggc aatgtaatca cgcgccagca ctgcatcggg
     4201 cttttgtcct gacagcagct gaaaaacgcc atcagcaata aagaagacgg ccagatcgtc
     4261 agttaatgcg gaagttgcca gtaaagcatc taaaccttcc cgaccagcag ctgtaccatg
     4321 aggtgcagta gaaaaaacaa acgcaattcg tttcatcaga actgtaccac cctgtcacag
     4381 gtcagcgagg cttccgccag cgcgccaagt ccgcttaagg taaatcccgg ctgaaggttt
     4441 gacgaagcca gccccagcct tcctgcttca gtttcatcga cgataccgcg gcgtaacgct
     4501 gccgctacgc agatattcag cgccacacca tgttgcgcat tcaactgttg ccagccgcgc
     4561 acaaggtcaa attcgtcact tgccggtgag gtcaattggt tagcgttata gaccccttcc
     4621 cgatagaaaa agacgctgct taactcatgg ccttctacta tcagcgcctg cgcaaactga
     4681 aaagcactac tcgcctgttg cgtaccgtat gctggcccgg tcaccacgat ggcaaaacgc
     4741 attacttatc ttgcccctgg aaatcaccgc ttttgaactg gcggatgtag agatagacag
     4801 tgtgtttgga gatgttcagg cggtcagcaa cctggttgat cgcgtcttta atatcgaaga
     4861 tccctttctc gtagagattc agcacgatct gacgattttt ggcgttatta gaaacattgc
     4921 gatcggcatt cacttcttcg atggtgaact ccagcgtttg ggtaaccaga tcttcaacag
     4981 aagaggcaaa gttgacgctt gaaccgacat ccggggtttc tggcggcaca aaagtgctca
     5041 taatctgcga gaagggaaca tcaagattca tattgatgca cagcagacca attacacgct
     5101 gttcgcggtt acgaatcgcg atagtcaggg acttcattaa tacgccgctt ttggcgcgag
     5161 taaagtagca tttagaaacg ctgctatccg ctcccgtcat atcgtgcagc atacgtagcg
     5221 ccaggtcagt aattggcgaa ccaatcttcc ggcctgtatg ttcaccgtta gcaatgcgaa
     5281 tggctgaaca ttttagatcc tgcaaagagt gcaaaacgat ttcacagtgg gagccaataa
     5341 gcatcgctaa cccgtccacc accgcttcgt aggatttcag aatatcaaaa tcggtctggt
     5401 cgaaaggacg ttgatccagt aaatccaact cactggtttc gttggttaaa agcgacctgg
     5461 acatgaaaaa aaacactcct tttcaggagc ctgtcgttaa cttttcaggg caggctcatt
     5521 aatgatgcgg gtaactaaat taatacagcg gaggttccgc tttccagcac taattatatc
     5581 cggcctgtaa taaaaaaacc gccgcctggt caggcggcgg ttcttaatgc ttatttttta
     5641 gcagaatctg cggctttcgc atcagcttcc ggctttgcat cagccttcgg cgctggtttc
     5701 acatccagca gctctacgtc aaacaccagg gtagaattcg gtgggatccc cggaacaccc
     5761 gctttgccgt aagccagttc tggtggaata accagtttga tcttaccgcc tttcttgatg
     5821 ttcttcagac cttctgtcca acccgggata acaccgtcca gacggaaaga aagcggttca
     5881 ccacgggtgt aagagttgtc gaactcttta ccgtcgatca gcgtaccttt gtagttcact
     5941 acaacagtat cgctgtcttt cggtgcttcg cctttaccgg cttctactac ctgataaacc
     6001 agaccagttg aagaggtttt cacacctttc tctttggcaa atttctcgcg gtactcttta
     6061 ccttttgctt cgttatcagc cgcgtctttt tccatcttcg cctgagcaga agacttcacg
     6121 cgagcttcga atgcttgcag agtctgttcg atctcttggt cggagagttt gctcttatca
     6181 gcaaatgcat cctgaacacc agcgatcagc tgatctttat ccagtttgat gcccagtttt
     6241 tcttgttctt ttagagagtt ttccatgtaa cgacccagcg aggcacccag tgcataagct
     6301 gatttctgat cgtcattttt gaacgctgct ttgctgtcag cagttgtagc aggttttgca
     6361 gcttcagcag caaaagtgat tggtgcatgc agggcaacgg ccattgtggt cgccagcagc
     6421 gttactttaa acagtgattt catccatatc tccaggatcg gggcatctca ccccagggtt
     6481 aactattatc agaagggtac tataaagcgt tgtcgaacaa atctacatac agacacgccc
     6541 tattatcatc tattttcaga ctctttttgt ttaaattagt ttcgatgacc gcgaaatgag
     6601 tgctgtctcg ggcagcaaag ttaagtagaa tccgcggcga ccattcgaca aaagaggtga
     6661 atcatgcagg atttatcatt ggaagcacgc ctggcagagc tggagagccg actggctttt
     6721 caggaaatca ccattgaaga actgaacgtc acggtgaccg ctcatgaaat ggagatggcg
     6781 aaactgcgcg atcatctgcg tctgctgacc gagaagttaa aagccagcca gccgtcgaac
     6841 atcgcgtcgc aggctgagga aacgccaccg ccacattatt gaggcgtaaa aaaagcgggg
     6901 attccccgct tttttgtcac cttttcggta ttagtggcaa ccacaaccgc cgttgccttt
     6961 accgccacag caaccttcgc caccgtgttc atgaccgtga tcgtggccat gaccgccgca
     7021 gcaaccgtcg tggtcgtgat cgtggtgatg atcgtgcgcg ccgtgaacgt ggccatgagc
     7081 cagttcttct tcggtcgctt cgcgaatcgc cacaacttca acgttgaatt tcaggttctg
     7141 accggccagc atgtggttac catcaaccac tacgtgatcg tcttcaaccg cagtgatttc
     7201 aaccggtacc ggaccctggt cagtttcagc caggaaacgc atacctacct gcagttcatc
     7261 aacgcccata aatacgtctt taggaacacg ttgcaccagg ttttcgtcgt actgaccgta
     7321 agcgtcgttc gcgccaacag cgacatcaaa tttgtcgcca acttcatgac cttccagcgc
     7381 cgtttccagg ccagagatca gggaaccgtg accatgcagg tagtccagcg gcgcactcac
     7441 cggagactca tcaaccaaca caccgtcttc tgtacgtacc tgataggcca ggctgaccac
     7501 caggtctttt gctactttca tgatatctcc tgagcatggg aagaatagtg gcgcagattg
     7561 tagcggaatt ctgcagccgt gtactcacta gcttaaaaaa actcggcgca tatcgctagt
     7621 ccggatgaaa aatcccgatc acttgctcat ctttgcgaac gtgatcgcgg gcttctttgt
     7681 ctgcttctcg catctgatgt ccgcacttaa cacattcaac aatatcaata ttattttcgc
     7741 gccacatcgc cattgaatcc tgcgcctgac aggccgggca ttttgcgccc gcgataaaac
     7801 gttttcggat tgccatcttt accctctact caaattcatc ccagccgtcc agctggcgtc
     7861 gttcttgttg catttcacgc tggaaaatct cttccagttc gcgtcgggct tccctggcgc
     7921 gagaaatttg cacggtatcg gcatgcattg ggatgagctc tcgcagcatc cgcatatcca
     7981 ggcggcgaaa atgcagttgc gctcgctgcg cctgatgcgg atgcatccca agcgtgacca
     8041 aagtcttgcg ccccagctct aacgcactgg agaatgtttc acgggaaaac tgcgtcaccc
     8101 ctgcctgtaa taactcatgc gcttccacac gtccgcgcgc tcgcgcaaga atatgcaaat
     8161 gcggaaagtg ctgctggcat atttccacca gcttcatggt gtcttccggc tcgttacagg
     8221 taatgacgat agactcagcg gcctctgcac ccgcagaacg taaaagatct acctgcgtgg
     8281 cgtcgccgta ataaactttg tagccatatt tgcgcatcag gttaacggcg ctgatatccc
     8341 gctccagcac ggtaatgcgc attttatttg ccatcagcaa acgaccaatc acctgcccaa
     8401 aacgcccgaa accgaccaca atcacctgcg gtttatcatc gttgacccat ggtttttcgt
     8461 cttcttcctc cggtccgtta aactggcggg atagccattt atctaccagc ttcatcagca
     8521 gcggcgtggt catcatggaa agcgtcaccg tcaccagcaa cagcgccatc tggtcgccct
     8581 ggaataaacg ttgtgaagaa gcggtagaaa agaggacaaa ggcaaactca ccgccctgac
     8641 tcaacacgcc agcaaactgc atccgctctg agctacgcac gccatacaat cgcgccagca
     8701 gatacagcac gagaattttc accgccacca gcacaaccac gcttatcact acccacaaca
     8761 gatgggtata aagcaccccg aggttgagtg acatcccgac agagataaag aacaaaccga
     8821 gcagcaagcc tttgaaggga tcgatagccg tttccagttc atggcgatat tcactttccg
     8881 ccagcagcac gcccgcaata aacgtaccga gcgccatcga cagccccagc gcatccataa
     8941 acaatgcgga acccaatacc agcagcagcg tcgcggcggt gaacacttcc cgcacgccag
     9001 aagctgcaat aaaacggaat accggacgca ataaatagcg cccgccaatc agcatgccga
     9061 caaacgccag caccttcatg ccgatcttca tccagtcgaa atgttcgtct gccgaacccg
     9121 ccaacaacgg cactaacgcc agtgccggga tcaccgccag atcctgaaac agcagtaccg
     9181 aaaacccgag ctggccggat tcgctgcgat tcatcccttt ctcacgcatc aattgcaacg
     9241 ccattgcagt tgaagacatc gcaaggccaa tgccaccgac caccgccgcc tgccaggcga
     9301 aatccgtcag catcaataat cccgccagca acgccgcgct taacagcacc tgtgccgcgc
     9361 ctacgccaaa aatcgaacgc cgcagttgcc aaagcttgga gggattcaac tcaaggccga
     9421 tgataaacat caggaatacc acgccgagtt ccgaaaagtg gaggatctca tcgacgtcgc
     9481 taataaaccc cagcccccat gggccaattg caatccctgc cagcaaatat cccaacacag
     9541 cgccaatacc cagccgcgat gccagcggca ccgcagccac cgccgcgaag agaaacagca
     9601 ctcctgcgag taaaaaatcg gaaccttcca tcagcggcct cctggagaca gcggatttgc
     9661 cagccagtca ccgtaggctc tggcgtgact cgccagctcc tgtgcgcttt gccgtctcgc
     9721 ccagtaaata atgatgggac ttaaccaatg catccggcac atgcccgccg ccagttcaaa
     9781 gggacgcaga acatcgctca tcgggtagcg attcaatgcg tcataacggt aagcactttc
     9841 cggctcgccg gtggtaatca cgctacgcca gtactttccc gccagttggt ttcctcccgg
     9901 cccgctggca aaaccacgac ttaatacccg gtccagccac tctttcagta gcgccgggca
     9961 gctataggta taaagaggat gctgaaagac aatcacctcg tgctcgcgca gtaatgcctg
    10021 ctcacggggg atatcaataa aaaaatcggg atagtgcgca taaaggtcgt gcacggtaac
    10081 attgctgagc tgcgtggccg gtttaagcag tacccggttt gccaccgagt cctgagattc
    10141 cggatgggca tacagcagca aaactttcgc tggctgagac atcatccccc tcccggtatg
    10201 gtttttgtgt atagtcgctg ttttgggcta ccattgcgcc cggtgcggca gctcgcccat
    10261 acattacatt atcataatga taagttaaca tagtctgaac atacggcgcc ttatgattgt
    10321 tttctcctcg ttacaaattc gtcgcggcgt gcgcgtcctg ctggataatg ccaccgccac
    10381 catcaaccct gggcagaaag tcggcctggt gggtaaaaac ggctgtggta aatctaccct
    10441 gctggcattg ctgaaaaatg aaatcagcgc cgacggcggc agctacacct ttccgggaag
    10501 ctggcaactg gcgtgggtga atcaggaaac gccggcgtta ccgcaagcgg cgctggaata
    10561 tgtcattgac ggcgaccgtg aatatcgtca actggaagcg cagctgcacg acgccaacga
    10621 acgtaacgac gggcacgcca ttgcgaccat tcatggcaag ctggatgcta ttgacgcatg
    10681 gagtattcgc tcccgtgccg ccagcctgct gcacggcctc ggtttcagca atgaacaact
    10741 ggagcgcccg gtaagtgatt tctccggtgg ctggcgtatg cgtcttaacc ttgcccaggc
    10801 gctgatttgc cgttcagact tgctgctgct cgacgaaccg actaaccacc tcgatctcga
    10861 tgccgttatc tggctggaaa aatggttgaa gagctatcag ggcacgctga tcctgatctc
    10921 tcacgaccgc gacttcctcg atccgatcgt tgataaaatt attcatatcg aacaacaaag
    10981 catgttcgag tacaccggca actacagttc gtttgaagta cagcgcgcca cccgtctggc
    11041 gcagcaacaa gcgatgtatg aaagccagca ggaacgcgtg gcacatctgc aaagttatat
    11101 cgaccgtttc cgtgccaaag ccaccaaagc gaagcaggcc cagagccgca ttaagatgct
    11161 tgagcgtatg gagctgattg cccccgcgca cgtcgacaac ccgttccgct ttagcttccg
    11221 cgcgccggaa agcctgccaa atccgttact gaagatggaa aaagtcagcg cgggctatgg
    11281 cgatcgcatt attctcgact cgattaaact gaacctggtg cccggctcgc gcattggtct
    11341 gttaggccgc aacggcgcgg gtaaatcgac attaatcaaa ctgttagccg gtgaacttgc
    11401 gccagtcagc ggtgaaattg gtctggcgaa agggatcaag ctcggctact tcgcccagca
    11461 tcaacttgaa tacctgcgcg ccgacgaatc acctattcaa catctggcac gtttagcgcc
    11521 gcaggagctg gaacaaaaac tgcgtgacta cctcggcggc tttggtttcc agggcgataa
    11581 agtaaccgaa gaaacgcgcc gcttctccgg tggggaaaaa gcccgcctgg tgctggcatt
    11641 aattgtctgg cagcgtccga atctgctgct gctcgacgaa ccgactaacc accttgacct
    11701 cgacatgcgt caggcactca ccgaagcatt aatcgagttt gaaggcgcgc tggttgtcgt
    11761 ttcgcacgac cgtcatttgc tgcgttccac cactgacgat ctctacctgg ttcacgatcg
    11821 taaagtcgaa ccgttcgacg gcgatctgga agattatcaa cagtggttga gcgacgtaca
    11881 aaaacaggaa aaccaggccg acgaagcgcc aaaagagaac gcgaacagcg cccaggcacg
    11941 taaagatcag aagcgtcggg aagcggagct gcgtgcgcaa acccagccac tgcgtaaaga
    12001 gattgcccgt ctggaaaaag agatggagaa gctgaacgcg caactggcgc aggcagaaga
    12061 gaaactcggc gacagcaaac tgtatgacca gagccgtaaa gcggagttga ccgcctgcct
    12121 gcaacagcaa gccagcgcca aatccggcct ggaagagtgc gaaatggcat ggctggaagc
    12181 ccaggagcag cttgagcaga tgttgctgga aggccaaagc aactgatggc gcagataacg
    12241 acgaccgatg ccaatgaatt cagcagcagt gctgaattca cccctatgcg cggctttagc
    12301 aattgtcatc tgcaaaccat gctgccgcgt ctgtttcgtc gcaaggtgaa attcaccccg
    12361 tactggcagc ggctggagtt gcccgacggc gattttgtcg atctcgcgtg gagtgaagcc
    12421 ccagcacagg cgcggcataa accgcgttta gtggtatttc acgggctgga aggcagtctc
    12481 aatagccctt acgcccacgg tctggttgag gcggcgcaaa agcgcggctg gctgggcgtg
    12541 gtgatgcatt ttcgcggatg cagcggtgaa ccaaaccgta tgcaccgcat ttaccattcg
    12601 ggcgaaaccg aagacgccag ttggttttta cgctggctgc aacgcgaatt tggtcatgcg
    12661 ccaacggctg ccgtcggcta ttcgctcggc ggtaatatgc tggcctgttt gctggcaaaa
    12721 gaaggcaatg atctcccggt tgatgcggcg gtgattgtct ctgcgccgtt tatgctggaa
    12781 gcctgtagct atcatatgga aaagggcttt tcccgcgttt atcagcgtta cttgctgaac
    12841 ctgttaaaag ccaatgccgc gcgcaagctg gcagcctacc ccggaacact gccgattaat
    12901 ctcgcgcagt taaaatcggt acgtcgcatc cgtgaatttg acgatctcat caccgccaga
    12961 attcacggct acgctgacgc tatcgactat tatcgtcagt gtagcgccat gccgatgctg
    13021 aaccggatcg ccaaaccgac gctgattatt cacgccaaag acgatccgtt tatggatcat
    13081 caggtgatcc cgaaaccgga aagtctcccc ccgcaggtgg agtatcaact gactgaacat
    13141 ggcggtcatg ttggctttat tggcggtaca ttacttcatc cgcaaatgtg gctggagtca
    13201 cgcattcctg actggttaac aacgtatctg gaggcgaaat catgttgatt ccgtggcaag
    13261 acctctcccc cgaaacgctg gaaaatttaa ttgaaagctt tgtgttacgt gaaggcaccg
    13321 attatggtga acatgagcgt acacttgaac agaaagtcgc cgacgtcaaa cgccagctac
    13381 agtgcggaga agcggtgctg gtatggtcgg agctgcacga aacggtcaat attatgccgc
    13441 gcagtcagtt tcgcgaataa cctgcaaccg tcggcggacg agataataat gatagctaag
    13501 tcatggagtt actatgtctg ccaaacatcc ggtcattgcg gtaacaggat ccagcggcgc
    13561 ggggaccacc accaccagcc tcgcgtttcg taaaatattc gcgcagttaa atctgcatgc
    13621 agctgaggtg gaaggcgaca gttttcaccg ttacacccgc ccggaaatgg acatggcgat
    13681 ccgcaaagcg cgcgacgccg ggcggcatat cagctacttc ggccccgagg ctaacgactt
    13741 cggcctgctg gaacaaacct tcattgaata cggtcagagc ggcaaaggga aatctcgcaa
    13801 atatctgcat acctacgacg aagccgtacc gtggaatcag gtaccgggga cattcacccc
    13861 ctggcaacct ttaccggaac ccactgatgt actgttttat gaaggtttac acggcggcgt
    13921 agtcacgcca cagcataacg ttgcgcagca tgtggactta ctggtcggcg tggtgcctat
    13981 cgttaacctt gagtggattc aaaaactgat ccgcgacacc agcgagcgcg ggcactcacg
    14041 agaagcagtg atggactcag tagtgcgttc aatggaagac tatatcaact acatcacacc
    14101 gcagttttcc cgcacccatc ttaacttcca gcgcgttccc accgtcgaca cttcaaaccc
    14161 gttcgcggca aaaggtatcc cgtcgctgga tgaaagcttt gtggtgatcc attttcgtaa
    14221 tctggaaggg atcgatttcc cttggctgct ggcgatgttg caaggctcat tcatttccca
    14281 catcaatacg ttagtggtac ctggcggcaa aatggggctg gcaatggaat taattatgct
    14341 gccgctggtg caacgattga tggaaggaaa gaaaatcgag taactttgct attatgccgg
    14401 ataaaatact atccggcttc acaacgggat agttaagtca cgcggcaacc acttcatacg
    14461 agtgagtaat attcaccgct ttttccagca tcaacgccac tgaacaatat ttctcggcag
    14521 agagatcaac cgcacgcgca accgctgcgt ctttcaggtc gcgaccggtg acgataaaat
    14581 gcagattaat gtgcgtaaac aggcgtggtg cctcttcgcg gcgttcagag gtcaatttta
    14641 cttcacaatc gaccacatcc tgacgccctt tttgcaggat cgaaaccaca tcgatggcac
    14701 tgcaaccacc cgccgccatc aacaccattt ccatcggact tggtgcttta tcgcctgagt
    14761 tgccgtccat taaaatctga tgaccagagg cggattcgcc cagaaaagtt aacccttcga
    14821 cccacttcac tcgcgcttgc atttttgcta ctccactgcg tcaattttcc tgacagagta
    14881 cgcgtactaa ccaaatcgcg caacggaagg cgacctgggt catgctgaag cgagacacca
    14941 ggagacacaa agcgaaagct atgctaaaac agtcaggatg ctacagtaat acattgatgt
    15001 actgcatgta tgcaaaggac gtcacattac cgtgcagtac agttgatagc cccttcccag
    15061 gtagcgggaa gcatatttcg gcaatccaga gacagcggcg ttatctggct ctggagaaag
    15121 cttataacag aggataaccg cgcatggtgc ttggcaaacc gcaaacagac ccgactctcg
    15181 aatggttctt gtctcattgc cacattcata agtacccatc caagagcacg cttattcacc
    15241 agggtgaaaa agcggaaacg ctgtactaca tcgttaaagg ctctgtggca gtgctgatca
    15301 aagacgaaga gggtaaagaa atgatcctct cctatctgaa tcagggtgat tttattggcg
    15361 aactgggcct gtttgaagag ggccaggaac gtagcgcatg ggtacgtgcg aaaaccgcct
    15421 gtgaagtggc tgaaatttcg tacaaaaaat ttcgccaatt gattcaggta aacccggaca
    15481 ttctgatgcg tctgtctgca cagatggcgc gtcgtctgca agtcacttca gagaaagtgg
    15541 gcaacctggc gttcctcgac gtgacgggcc gcattgcaca gactctgctg aacctggcaa
    15601 aacaaccaga tgctatgact cacccggacg gtatgcaaat caaaattacc cgtcaggaaa
    15661 tcggtcagat tgtcggctgt tctcgtgaaa ccgtgggacg cattctgaag atgctggaag
    15721 atcagaacct gatctccgca cacggtaaaa ccatcgtcgt ttacggcact cgttaatccc
    15781 gtcggagtgg cgcgttacct ggtagcgcgc cattttgttt cccccgatgt ggcgcagact
    15841 gatttatcac cccgatatca actatgcact tcgacaaacg ctggtgctat gtttgcccgt
    15901 ggccgttggg ttaatgcttg gcgaattacg attcggtctg ctcttctccc tcgttcctgc
    15961 ctgttgcaat attgcgggcc ttgatacgcc tcataaacgt tttttcaaac gcttaatcat
    16021 tggtgcgtcg ctgtttgcca cctgtagctt actgacacag ctgctactgg taaaagatgt
    16081 tcccctgccc tttttgctga ccggattaac gctggtactt ggcgtcactg ctgagctggg
    16141 gccattgcac gcaaaattgc ttcctgcatc gctgctcgcc gccattttta ccctcagtct
    16201 ggcgggatac atgccggtct gggaaccatt gctcatctat gcgttgggca ctctctggta
    16261 cggattgttt aactggtttt ggttctggat ctggcgcgaa caaccgctgc gcgagtcact
    16321 aagtctgctg taccgcgaac tggcagatta ttgtgaagcc aaatacagcc tgcttaccca
    16381 gcacaccgac cctgaaaaag cgctgccgcc gctgctggtg cgccagcaaa aagcggtcga
    16441 tctaattacc cagtgctatc agcaaatgca tatgctttcc gcgcaaaata atactgatta
    16501 caagcggatg ctgcgtattt tccaggaggc gctggattta caggaacata tttcggtcag
    16561 tttgcatcag ccggaagagg tgcaaaagct ggtcgagcgt agccatgcgg aagaagttat
    16621 ccgctggaat gcgcaaaccg tcgccgctcg cctgcgcgtg ctggctgatg acattcttta
    16681 ccatcgcctg ccaacgcgtt ttacgatgga aaagcaaatt ggcgcactgg aaaaaatcgc
    16741 ccgccagcat ccggataatc cggttgggca attctgctac tggcatttca gccgcatcgc
    16801 ccgcgtgctg cgcacccaaa aaccgctcta tgcccgtgac ttactggccg ataaacagcg
    16861 gcgaatgccg ttacttccgg cgctgaaaag ttatctgtca ctaaagtctc cggcgctacg
    16921 caatgccgga cgactcagtg tgatgttaag cgttgccagc ctgatgggca ccgcgctgca
    16981 tctgccgaag tcgtactgga tcctgatgac ggtattgctg gtgacacaaa atggctatgg
    17041 cgcaacccgt ctgaggattg tgaatcgctc cgtgggaacc gtggtcgggt taatcattgc
    17101 gggcgtggcg ctgcacttta aaattcccga aggttacacc ctgacgttga tgctgattac
    17161 caccctcgcc agctacctga tattgcgcaa aaactacggc tgggcgacgg tcggttttac
    17221 tattaccgca gtgtataccc tgcaactatt gtggttgaac ggcgagcaat acatccttcc
    17281 gcgtcttatc gataccatta ttggttgttt aattgctttc ggcggtactg tctggctgtg
    17341 gccgcagtgg cagagcgggt tattgcgcaa aaacgcccat gacgctttag aagcctatca
    17401 ggaagcgatt cgcttgattc ttagcgagga gccgcaacct acgccactgg cctggcagcg
    17461 aatgcgggta aatcaggcac ataacactct gtataactca ttgaatcagg cgatgcagga
    17521 accggcgttt aacagccatt atctggcaga tatgaaactg tgggtaacgc acagccagtt
    17581 tattgttgag catattaatg ccatgaccac gctggcgcgg gaacaccggg cattgccacc
    17641 tgaactggca caagagtatt tacagtcttg tgaaatcgcc attcagcgtt gtcagcagcg
    17701 actggagtat gacgaaccgg gtagttctgg cgatgccaat atcatggatg cgccggagat
    17761 gcagccgcac gaaggcgcgg caggtacgct ggagcagcat ttacagcggg ttattggtca
    17821 tctgaacacc atgcacacca tttcgtcgat ggcatggcgt cagcgaccgc atcacgggat
    17881 ttggctgagt cgcaagttgc gggattcgaa ggcgtaatac aggttaaacc atcggataag
    17941 gcattcacgc cgcatccaca tcttttgcct gatgcgtctt atttacgccc caatcacctt
    18001 cgccaccgcg tgggcgaaac gttgcatccc ttcatcgata tccgcatctt ccaccaccag
    18061 cgacggtgca aaacgcatca catccggtcc ggcattcagc accattacgc caacctctgc
    18121 acccgcatac aggaaatcac gcgcccgacc tttgtagtgt ggtttcagct ccgcgccaat
    18181 taacagcccc ataccgcgaa tatcgctaaa cacatcgtac tgctgatcga tcttctgcaa
    18241 atggtcaaca aaacgctggc gtttcgcctg aatgccttcc agcacttcag gggtattgat
    18301 gatatcaaac gccgcgcccg ctactgcaca ggccagagga ttaccgccgt aggtggaacc
    18361 gtgagaacca ggatgaaacg cagaagcaat ttccgccgtg gtcagcatgg cgctaatcgg
    18421 gaagccgccg cctaacgctt tcgcagaagt cagaatatcc ggcgtcacac cgtagtacat
    18481 gtaagcaaac aaatcgccag tccgccccat cccgcactgc acttcatcaa acaccaataa
    18541 tgcctgatgt tgatcgcaca gctcgcgcaa gccctgcaaa aactctggcg tcgctgccgt
    18601 cacaccgccc tcgccctgga tcggctcaac caccaccgca caggtgtgat catccatcac
    18661 cgctttcact gcatggagat cgttaaaggg aacgtggatg atgtctgccg gtttcggccc
    18721 aaagccgtcg gaatattttg gctgcccacc caccgaaacg gtaaacagcg agcgaccatg
    18781 aaaagcgtta tggaaggcaa taattttggt tttgaacggg ctatgacgca cacaggcgta
    18841 atggcgtgcc agtttaaagg cggtttcgtt agcttccgtg ccggagttca taaacaccac
    18901 gcgttcggca aacgtcgcct caatcagttt gcgcccaaga cgcagcgccg gttcattggt
    18961 gaaaacgtta ctgatatgcc acagagtttc gccctgggtt tttaacgcgt tcaccagcgc
    19021 aggatggcaa tggcccaacg ccgtaactgc aatgccaccc gcgaaatcga catactcctt
    19081 gccttgctga tcccagattc ggctgccctg accttttacc ggaataaact ctgccggagc
    19141 ataaatcggc aggatcactt catcgaaagt cgcgcgtgta attgctgttt gttcaattgc
    19201 catctcatga tcaccctgtt acgcataaat aaatgtgaaa ttataatcac aaaatatgca
    19261 taaaaaatca ctgaatggca atcggaaatc agagatgcag gaaattagcc agcagttgat
    19321 gtccttgttc gctaagaata ctttctggat ggaactgcac accttccaga tcccactggc
    19381 gatggcgaat ccccataatc tcgcgggttt cgctccaggc cgtcacgtca aagcacgctg
    19441 gtaatgagcc aggttccacc accagcgaat ggtagcgtgt cacggtaagt ggatttgcca
    19501 gcccccgaaa tacgccctca ccgttatgtg taatcggcga ggttttgccg tgcatgacct
    19561 ttgcggcgcg cacaacttta ccgccaaatg cctgcgccat tgcctgatga ccgaggcaga
    19621 cgccaagaat cggcaagcgc ccggcatagt ggcgaataac gtcaagggag atcccggctt
    19681 catctggcgt acaggggccg ggtgagatga caattttttg tggtttaagg gcgtcgatat
    19741 ccgccaacgt caacgcatcg ttgcgcttaa ccagcacatc cgcccccagt tcacaaaagt
    19801 actggtagag gttccaggta aaagaatcgt agttatcaat aagcaggatc atggcggctc
    19861 cggtacgaaa gaaccgcgct attttactca gattccccgg cttcgcttac cactttgcta
    19921 aatatcatct gcagtgcggt gagatccccc attgccccac tctgatttgc ctgattccag
    19981 gcctcttttt cgataccctg ccagctcagt tgatatccgg catgaatcgc cagttgctcg
    20041 aagaagatcc gctgtgccag accacttccc acccggaagg gatgcagcac gttgatttca
    20101 caatagtaat gcgccagccg ctcgacgaac ttcgttttct ccaggccaac cagataacct
    20161 tcttcctcca gatcctgcat cagggcattg ccctcttttt cgatataagc aaagtggcag
    20221 aacggcgtat cgccctgata aatatcaact tcacgcagtt gccctgccca gtcgaaaata
    20281 tcctgataca gctggcgatg gatagttcgc aaatgcggta aaccgcgcac cagcggacca
    20341 agctcaatgg tcgcagcacg cagcgccgtc atttcgtaag cggcctgttc cagccgttgt
    20401 tgctggcgga tgttcagccg gttacgcatg atatcaaggc ctggataaag atacggatcg
    20461 cgcccttcgc cgaatttatc gctcatagtg cctcctcagc tctacaaggc gcgctaaagc
    20521 ctctgcggca gtaagagtga ctaaaggcat ctcgacgcct tcaagacggc gactggcctg
    20581 gaaattacga ttacgctgaa gctcccagag acgggacttt tgcttatcgg tgagtttctt
    20641 cacttgatgc ctccctgaac gtgtccattt gccacaagta taagcggcaa atccgggtta
    20701 cgccgggaga gcagaagcgc gggcaagatt gcccgcgcga gaaatcatta cggcaggact
    20761 ttagcggaaa ggataactac cggttttgac ggcacattct ggtacggacc aacgtcatga
    20821 gtcggcacct gggaaatctt atcggccacg tccatgcctt tcaccacttt accaaatacc
    20881 gcgtaaccga aatcacgctg accatggtca aggaaggcgt tatcggcaac gttgataaag
    20941 aactggctgg tggcgctgtc tttgtcagcg gtacgcgcca tcgcgatggt gccacgcgtg
    21001 ttgcgcaggc cgttatcggc ttcatttttg attggcgggt ttggtttttt ctgctgcatc
    21061 tgctcggtga aaccgccgcc ctgaatcata aagccaggaa tgacgcggtg aaaggtagtg
    21121 ttgttataaa aaccgctgtt cacataatcg acaaagtttt gcacagacac tggcgctttt
    21181 tgtttatcca gctccagttc gatgttacca gctgaggttg tcaacaatac gtgcgggtcc
    21241 cctttcgctg ccattgctgc gggagaaaga gcagaaagag cgaaaacagc agccatcgcc
    21301 gccagggtcg atttgaacat gagatttcct taacaaacag agcagaaaaa aagcaagtga
    21361 agagattcta aatagccttc aagaacaagg ccatcccttt acctaatttt acgtatctga
    21421 aatatatgta acctgacgat cactttttat tccgtgatca aaatcatctc ttaaaatgca
    21481 atttagcaac cgattgcaat aaaacattta aacagatcac aaaataacct aaaatcgccc
    21541 gctcgcggca tatcccgcta tgccatttta tttttgaacg caggccaatc atgactaaca
    21601 gcaatcgcat caagctcaca tggattagct ttctctccta cgcactgact ggtgcgttgg
    21661 ttattgtcac cgggatggtg atgggaaata tcgccgatta tttcaatctg cctgtttcca
    21721 gtatgagtaa caccttcacc ttcctcaacg ccggcatttt aatctctatc ttcctcaacg
    21781 cctggctgat ggaaatcgtc ccgttgaaaa cgcagttacg ttttggcttt ctcctgatgg
    21841 tgctggcggt tgccggtttg atgttcagcc acagcctggc gctgttctcg acggcgatgt
    21901 tcattctcgg ggtggtcagc ggcattacta tgtcgattgg tacattcctg ataacacaaa
    21961 tgtatgaagg gcgtcagcgc ggttcccgcc tgttatttac tgactccttc ttcagtatgg
    22021 ctgggatgat tttcccaatg atcgccgcgt ttctactggc gcgcagcatt gagtggtact
    22081 gggtttatgc ctgcatcggg ctggtgtatg tcgctatttt tattctgacc ttcggctgtg
    22141 agttcccagc actgggcaaa catgcaccaa aaacggatgc tccggtagag aaagaaaagt
    22201 gggggatcgg cgtactgttt ctctctgttg cggcgctgtg ctacatcctc ggtcagttag
    22261 gttttatctc ctgggtgcct gagtatgcca aaggcctggg catgagcctg aacgacgcgg
    22321 gcacgctggt gagtaacttc tggatgtcat acatggtcgg catgtgggcg ttcagcttta
    22381 ttcttcgctt ctttgatttg caacgcattc tgaccgtact ggctggtctg gctgcgattc
    22441 tgatgtacgt ctttaacacc ggaacaccag cacatatggc gtggtcaatt ctcgctctgg
    22501 gcttcttctc cagcgcgatc tataccacca tcatcaccct gggttcacag cagaccaaag
    22561 taccgtcgcc aaaactggtt aactttgtcc tgacctgcgg aaccatcggt actatgttga
    22621 cctttgtggt taccggcccg attgttgaac atagcggtcc gcaggcggca ctgcttacag
    22681 caaacggtct gtacgctgtc gtctttgtga tgtgcttcct gttaggtttc gtcagccgtc
    22741 accgtcagca taacaccctg acctctcatt aattgctaat gccggacggc actatcgtcg
    22801 tccggccttt tcctctcttc ccccgctacg tgcatctatt tctataaacc cgttcatttt
    22861 gtccattttt tgcacaaaca tgaaatgtca gacaattccg tgacttaaga aaatttatac
    22921 aaatcagcaa tatacccctt aaggagtata taaaggtgaa tttgatttac atcaataagc
    22981 ggggttgctg aatcgttaag gtaggcggta atagaaaaga aatcgaggca aaaatgagca
    23041 aagtcagact cgcaattatc ggtaacggta tggtcggcca tcgctttatc gaagatcttc
    23101 ttgataaatc tgatgcggcc aactttgata ttaccgtttt ctgtgaagaa ccgcgcatcg
    23161 cttatgaccg cgtacacctc tcgtcttact tctctcacca caccgccgaa gagctgtcgc
    23221 tggtgcgcga aggcttctac gagaaacacg gcatcaaagt tctggtcggc gaacgcgcta
    23281 tcaccatcaa ccgtcaggag aaggtgattc actccagcgc cggacgtacc gttttttatg
    23341 acaagctgat catggcaacc ggttcctacc cgtggatccc accaatcaaa ggttctgata
    23401 ctcaggactg ctttgtctat cgcaccattg aagacctcaa cgccattgaa tcctgcgccc
    23461 gtcgcagcaa acgcggtgcc gttgttggtg gcggcctgtt aggtctggaa gccgctggcg
    23521 cgctgaaaaa cttaggtatt gaaacccacg ttatcgaatt tgcccctatg ctgatggcgg
    23581 aacagcttga tcagatgggt ggcgagcagc tgcgtcgcaa aatcgaaagt atgggcgtgc
    23641 gcgttcacac cagcaaaaac acccttgaga ttgtgcagga aggtgtcgaa gcacgtaaaa
    23701 ccatgcgttt tgccgacggc agcgaactgg aagtcgactt tatcgtcttc tctaccggta
    23761 tccgtccgcg cgataagctg gcaacccagt gtggtctgga cgttgctccg cgtgggggta
    23821 tcgtcattaa tgattcctgc caaacttccg atccggatat ctacgccatc ggtgaatgcg
    23881 caagctggaa caaccgtgta tttggtctgg tagcacctgg ctacaaaatg gcgcaggtcg
    23941 ccgttgacca tattctcggt agcgaaaacg cctttgaagg tgctgacctt agcgccaagc
    24001 tgaaactgct gggcgtggac gtaggcggta ttggtgatgc tcatggtcgc acgcctggcg
    24061 cacgtagcta cgtttacctc gacgaaagca aagagatcta caaacgcctg atcgtcagcg
    24121 aagacaacaa aaccctgctc ggtgcggtac tggtgggcga taccagcgac tacggtaact
    24181 tgctgcaact ggtgctgaat gctatcgagc tgccggaaaa cccggattct ctgattctgc
    24241 cggcgcactc gggtagcggt aagccgtcta tcggtgttta taaactgccg gacagcgcgc
    24301 aaatctgctc ctgcttcgac gtcactaaag gtgatctgat tgctgccatc aacaaaggct
    24361 gccacacagt tgcggcgctg aaagctgaaa ccaaagcggg tactggctgc ggtggctgta
    24421 tcccgctggt cactcaggta ctgaacgcgg aactggcgaa acagggcatc gaagttaaca
    24481 acaacctgtg cgaacacttt gcttattcgc gtcaggaact gttccatttg atccgcgttg
    24541 aaggcattaa aaccttcgaa gaactgctgg cgaaacacgg caaaggctac ggttgtgaag
    24601 tttgtaaacc gaccgtcggt tcgctgctgg cctcctgctg gaacgaatac attctgaagc
    24661 cagaacatac tccgctgcaa gattctaacg acaacttcct cgctaacatc cagaaagacg
    24721 ggacctactc ggtgatcccg cgttctccgg gcggtgaaat caccccggaa gggctgatgg
    24781 cagtgggtcg tatcgcgcgt gaatttaatc tctacaccaa aatcaccggt tcccagcgcc
    24841 tggcgatgtt tggcgcacag aaagacgatc tgccagagat ctggcgtcag ctgattgaag
    24901 ccggcttcga aaccggtcat gcctatgcga aagcactgcg tatggcgaaa acctgcgtgg
    24961 gtagcacctg gtgccgctac ggcgttggcg acagcgtcgg cctcggcgtg gaactggaaa
    25021 accgctacaa aggcatccgt acgccgcaca aaatgaagtt cggtgtctcc ggctgtaccc
    25081 gtgaatgttc agaagctcag ggtaaagacg tgggtattat cgccactgaa aaaggctgga
    25141 acctgtatgt ttgcggcaac ggcggcatga aaccgcgtca tgcggatctg ctggcagcgg
    25201 atatcgatcg cgaaacgctg atcaaatatc tcgaccgctt catgatgttc tacatccgta
    25261 ccgccgacaa actgacgcgt accgcgccgt ggttagaaaa cctcgaaggc ggcatcgatt
    25321 acctgaaagc ggtgatcatt gacgacaaac tggggctgaa cgctcatctg gaagaagaga
    25381 tggcgcgcct gcgtgaagcg gtagtgtgtg agtggactga aacggtcaat acaccgtccg
    25441 cgcagactcg cttcaaacac ttcatcaaca gcgacaagcg tgacccgaac gtgcagatgg
    25501 tgccagagcg cgaacagcac cgtccggcaa cgccgtatga acgtatcccg gtaactctgg
    25561 tggaggacaa cgcatgagcc agtggaaaga catctgcaaa atcgatgaca tcctacctga
    25621 aaccggcgtc tgcgcgctgt taggcgacga gcaggtcgcg attttccgcc cgtatcacag
    25681 cgaccaggtg tttgcgatca gcaacatcga tccgttcttc gagtccagcg tgctgtcacg
    25741 cgggctgatt gcggaacacc agggcgagct gtgggtcgcc agcccgctga aaaaacagcg
    25801 ttttcgctta agtgatggct tgtgcatgga agacgaacag ttttccgtca aacattacga
    25861 agcacgagtg aaagacggcg tggtgcagct gcgcggttaa tgttttaacg ggaggcgcaa
    25921 tgcctcccct ttttgcatgg tcctgtaatg accttcggta tattgcagga cattttttaa
    25981 actttttgtt ttattttttt gtttttattt tttaaaggat aatcaaatgt ttacagacac
    26041 tattaataag tgtgcggcta acgctgcgcg cattgcacgc ctgtcggcaa ataacccgct
    26101 cggcttttgg gtcagctccg ccatggcggg cgcgtatgtg ggtcttggga tcatcttgat
    26161 tttcacgctc ggtaatttgc tcgacccgtc cgtacgtccg ctggtgatgg gcgcgacctt
    26221 tggtatcgcc ttaacgctgg tgattatcgc cggttctgaa ctgttcaccg gacacaccat
    26281 gttcctcacc tttggggtaa aagcgggcag cattagccac gggcaaatgt gggcaatcct
    26341 gccgcaaacc tggctgggta acctggtcgg ttccgtcttc gttgccatgc tctatagctg
    26401 gggcggcggt agcctgctgc cggtagatac cagcatcgtt cactccgtcg cgctggctaa
    26461 aaccactgca ccggcaatgg tactcttctt caaaggtgca ttgtgtaact ggctggtttg
    26521 cctggcaatc tggatggcgc tgcgcactga aggggcggcg aaatttatcg ctatctggtg
    26581 gtgtctgctg gcatttatcg cgtccggcta cgagcactct atcgctaaca tgacgctgtt
    26641 cgcgctctcc tggttcggca accacagcga agcctacacg ctggcgggta ttggtcataa
    26701 cctgctgtgg gtgacgctgg gtaatacttt atcaggtgcc gtattcatgg gattgggtta
    26761 ttggtatgct acgccgaaag cgaatcgtcc ggttgcggac aaatttaatc aaactgaaac
    26821 ggctgccggt taattactaa ggggttttta cgtggatcat ttgcctatat tttgccaatt
    26881 acgcgatcgc gactgtctga ttgtcggcgg tggtgatgtc gcggaacgca aagcaaggtt
    26941 gctgttagac gcaggcgctc gcttaacggt gaatgcatta gcgtttattc cacagttcac
    27001 cgcatgggca gatgcaggca tgttaaccct cgtcgaaggg ccatttgatg aaagccttct
    27061 cgacacctgc tggctggcga ttgcagcgac ggatgatgac acgcttaacc agcgcgtcag
    27121 cgaagccgct gaagctcgtc gcatcttctg taacgtagtc gatgcgccga aagccgccag
    27181 ctttattatg ccgtcgatta ttgaccgctc accgctaatg gtagcggtct cctctggcgg
    27241 cacctctccg gttctggcgc gcctgttgcg cgaaaaactc gaatcactgc tgccgttgca
    27301 tctgggccag gtggcgaaat atgccgggca attacgcggg cgagtgaaac aacagttcac
    27361 cacgatgagt gagcgtcgcc gtttctggga gaaattgttc gttaacgacc gcctggcgca
    27421 gtcgctggca aacaacgatc agaaagccat tactgaaacg accgaacagt taatcaacga
    27481 accgctcgac catcgcggtg aagtggtgct ggttggtgca ggtccgggcg atgccgggct
    27541 gctgacgctg aaaggactgc aacaaattca gcaggcagat gtggtggtct acgaccgtct
    27601 ggtttctgac gatattatga atctggtacg ccgcgatgct gatcgcgttt tcgtcggcaa
    27661 acgcgcggga taccactgtg taccgcagga agagattaac cagatcctgc tgcgggaagc
    27721 gcaaaaaggc aaacgcgtgg tgcggctgaa aggtggcgat ccgtttattt ttggccgtgg
    27781 tggcgaagag ctggaaacac tgtgcaacgc gggtattccg ttctcggtgg ttccgggtat
    27841 taccgcagct tctggttgct ctgcctattc gggtattcca ctcacgcatc gcgattatgc
    27901 ccagagcgta cgcttaatta ccggacactt aaaaaccggt ggcgagctgg actgggaaaa
    27961 cctggcggca gaaaaacaga cgctggtgtt ctatatgggg ttgaatcagg ccgcgactat
    28021 tcagcaaaag ctgattgaac acggtatgcc tggcgaaatg ccggtggcaa ttgtcgaaaa
    28081 cggaacggca gtcacgcagc gcgtgattga cggtacgctc acgcaactcg gtgaacttgc
    28141 tcagcaaatg aacagtccat cgctaattat tattggtcgg gttgttggcc tgcgcgataa
    28201 actgaactgg ttctccaacc attaatttaa cccgggccag agaattctgg ccttcttaat
    28261 gactcttttt atatattcag caaataaaac ataacccctt aataaatatt atctgctaat
    28321 gggttatatt caccgttaca gcgacttaca aaattaagtc attcgctcac tgacgccaat
    28381 taaatattcc tgtatcgttc ttaacgagca gtctgcttaa tattcatata atcaatgaat
    28441 attaattaat atataataca tagggaatgt aaatgaacaa atttattaaa gttgcactgg
    28501 taggtgcagt actggctacg ttaactgcat gtactggtca tattgaaaac cgtgataaga
    28561 actgctctta cgactacctg ctgcacccgg caatttctat ttctaaaatc attggcggtt
    28621 gcggtcctac tgcacagtaa gtctctgaca gaaatcggct aacaccggtt taattgcccg
    28681 acgccctctg gattttccga gggcgtattt gtttctatat ccttcttgct ccccacccgt
    28741 aaaaatattt tcactttaat ttcaattgga taactacatt cattagccac agcgcgatcc
    28801 tgtgcgaaat tttgtgatct tccttcgcat tacataacat catatgttgt tatattcatc
    28861 atgcattgtc atgttacctt ttaaatgact gcaaactctc ccctacaacg tgttggacaa
    28921 gaaaaaggta tcgctatggg aagccaggaa ctccaacgca agctcggatt ttgggccgtt
    28981 cttgcaatcg ccgtcgggac aaccgtcggc tccggtattt ttgtatctgt gggtgaagtg
    29041 gcaaaagcag cgggcacgcc gtggcttacg gtgctcgcgt ttgtcattgg cgggttaatt
    29101 gtgatcccgc aaatgtgcgt ctatgcggaa ctatccaccg cttatccgga aaatggcgca
    29161 gattatgttt atctgaaaaa tgccggaagc cgaccgctgg ctttcctctc cggctgggcc
    29221 agcttctggg ccaacgatgc gccgtcattg tcgattatgg cgctggcgat tgtcagcaat
    29281 cttggctttt taacgcctat cgatccgttg cccggtaaat ttatcgccgc cggattaatt
    29341 atcgccttta tgttgctaca cctgcgctcc gttgaaggcg gcgcagcgtt tcagacgcta
    29401 attaccatcg ccaaaattat cccgttcact atcgtcattg gccttgggat cttctggttt
    29461 aaagcggaga attttgccgc ccctacctcc actgcgattg gcgcaacggg cagctttatg
    29521 gcgctgctgg cggggatctc tgccaccagt tggtcgtata ccggcatggc ctctatctgt
    29581 tatatgaccg gcgaaattaa aaaccccgga aaaaccatgc cacgagcgct gattggttcc
    29641 tgtctgctgg ttctggtgct ctacaccctg ctggcgctgg tgatttccgg cctgatgccc
    29701 ttcgacaaac tcgccaattc tgaaacgccg atttccgacg ccctgacctg gatccccgca
    29761 ctcggcagca ccgctgggat ctttgttgcc atcacggcga tgatcgtcat tcttggttcg
    29821 ctttccagct gcgtgatgta ccagccgcgg ctggaatacg cgatggcgaa agacaacctg
    29881 ttctttaaat gcttcggcca tgtgcatccg aaatacaaca cgccggatgt ctccatcatc
    29941 ctgcaagggg cgctggggat cttcttcatc ttcgtttccg atctcaccag cctgctgggt
    30001 tatttcaccc tggtgatgtg tttcaaaaat accctcacct tcggctccat catctggtgt
    30061 cgtaaacgcg acgattacaa accgctgtgg cgtactccgg ctttcgggct gatgaccacc
    30121 ctcgccattg cgtcaagcct cattctggtc gcctcaacct ttgtctgggc accgattccc
    30181 ggccttatct gcgccgtcat cgttattgct actggtctgc ctgcttacgc cttctgggcg
    30241 aagcgtagcc gccagctcaa cgctttgtcg taatgttatc tggagaaaat aaaatgttgg
    30301 atattgataa aagcaccgtg gactttctgg tcaccgaaaa tatggttcag gaagtggaaa
    30361 aagttctcag ccatgacgtt ccgctggtgc acgccatcgt ggaagagatg gtgaagcgcg
    30421 acattgatcg tatttatttc gttgcctgcg gctcgccact caacgcggcg caaacggcga
    30481 aacatctggc ggatcgcttt tccgatcttc aggtctacgc catttccggc tgggagttct
    30541 gcgataacac cccgtatcgc ctcgacgatc gttgcgcggt gattggcgtt tctgactacg
    30601 gtaaaacgga agaggtaatc aaagcgctgg agctgggccg ggcctgcggc gcactcactg
    30661 cggcgttcac caaacgcgcg gacagcccga ttacctcggc ggcggaattt agcattgatt
    30721 atcaggccga ctgtatctgg gaaattcacc tgctgctctg ctacagcgtg gtgctggaga
    30781 tgatcacccg cctcgcgccg aacgcggaaa tcggcaagat caaaaacgat ctcaagcagt
    30841 tgccgaatgc gctcggtcat ctggtacgca cctgggaaga aaaaggccgc cagcttggtg
    30901 aactggccag ccagtggccg atgatttata ccgttgctgc gggtccgctg cgtccgctgg
    30961 gttacaaaga aggcattgta acgctgatgg aatttacctg gacgcacggc tgcgtgattg
    31021 agagcggaga gttccgccat ggcccactgg agattgtcga accgggcgtt ccgttcctgt
    31081 tcctgctcgg caatgatgaa agtcgccaca ccaccgaacg cgccattaac tttgttaaac
    31141 agcgtactga caacgtgatc gtcatcgatt acgccgaaat ttcgcaaggg ctgcacccgt
    31201 ggctggcacc gttcctgatg ttcgtgccaa tggagtggct ctgctactac ctgtctattt
    31261 acaaagatca caacccggat gaacgccgct attacggtgg tctggtggaa tattaatccc
    31321 tctcccggcc cggcaacggg ccggatttac gcaaggagtt acccgatgaa aacaggtatg
    31381 tttacctgcg gccaccagcg gctgcctatt gaacacgcat ttcgtgatgc aagcgagctg
    31441 ggttacgacg gcatcgaaat ttggggcggt cgcccgcacg cgttcgcgcc ggacttaaaa
    31501 gcgggcggca tcaaacaaat caaggcgctg gcgcagacgt atcagatgcc gattatcggc
    31561 tatacgccag aaaccaacgg ctatccgtat aacatgatgc tgggcgatga acatatgcgt
    31621 cgcgaaagcc tcgacatgat caagctggcg atggatatgg caaaagagat gaacgcgggt
    31681 tatacgctga tttccgcggc ccacgcgggc tatctcacgc cacctaatgt tatctgggga
    31741 cggctggcag agaacctaag cgaactgtgt gagtacgcgg aaaacatcgg catggatcta
    31801 atcctcgaac cgttaatgcc gtatgaatcg aacgtcgtgt gtaatgctaa tgatgtgctt
    31861 catgcgctgg cgctggtgcc ttcgccgcgc ctgttcagca tggtcgacat ttgcgcgccg
    31921 tatgtccagg cggaaccggt gatgagttat ttcgacaaac tgggcgataa attacgtcat
    31981 ctgcatattg tcgacagcga cggggccagc gacacgcatt acattcctgg agaaggcaaa
    32041 atgccgctga gggaactgat gcgcgatatt atcgaccggg gctatgaggg ttactgtacg
    32101 gtggagctgg tgacgatgta tatgaacgag cccagactct atgcccgcca ggcgctggaa
    32161 cgctttcgcg cgctgctgcc ggaggatgag agatgaaaac cctggcgaca atcggcgata
    32221 actgcgtcga tatctacccg caactgaata aagcgttttc tggcggtaat gcggtcaatg
    32281 tggcggtgta ctgcactcgc tacggcatac agccgggatg cattacctgg gtgggtgacg
    32341 atgactacgg cacaaagctg aagcaggatc tcgcccgcat gggcgtcgat atcagccatg
    32401 tccatacgaa acacggcgtt accgcacaaa ctcaggtgga actgcacgac aatgatcgcg
    32461 tttttggcga ctacaccgaa ggcgtgatgg ccgactttgc cctgagtgaa gaggattacg
    32521 cctggctggc gcagtatgac attgtgcacg cggcaatctg gggacatgcg gaagacgcat
    32581 tcccacagct gcacgctgcg ggcaaactta ccgctttcga cttctccgac aagtgggaca
    32641 gcccgctctg gcagacactg gtgccgcatc tcgattttgc ctttgcctcc gcaccgcaag
    32701 aagacgaagc gctgcgtctg aagatgaaag cgattgttgc ccgtggcgca ggaacagtga
    32761 ttgtcacgct gggtgaaaac ggcagcattg cctgggatgg cgcgcagttc tggcgtcagg
    32821 ctcctgaacc ggtgacggtt atcgacacca tgggtgccgg agattcgttc attgccggat
    32881 tcctttgcgg ctggtctgcg gggatgacat taccgcaggc gatggcgcag ggaacggcgt
    32941 gcgcggcgaa aaccattcag taccacggtg cctggtaggt ataacgttgg cgtgagcatc
    33001 ttcacgccaa cgtgctgtta cttgccggaa aacgacccta taatccgagt aattcattct
    33061 ttatttcagg gtcgattatg tcagctacgg accgctactc tcatcaactc ctctacgcta
    33121 ccgtccgcca gcggctgctg gatgatatcg cgcagggggt ttaccaggcc gggcaacaga
    33181 tccctaccga aaacgagctt tgtacacaat ataacgtcag ccgcattacc attcgcaaag
    33241 ccatcagcga cttagtggca gacggcgtac tgatccgctg gcagggaaaa ggcacctttg
    33301 tacaaagcca gaaagttgaa aacgccctgc ttactgtcag tggttttacc gattttggcg
    33361 tctcacaagg caaggcgacg aaagagaaag tgatcgaaca ggaacgggtc agtgccgcgc
    33421 cgttttgcga aaagctgaac atccccggaa acagcgaagt gttccatctc tgccgggtga
    33481 tgtatctcga taaagagccg ctgtttattg atagttcatg gatcccgctg tcgcgttatc
    33541 ctgactttga tgagatttac gtcgaaggaa gctccaccta tcagttattt caggagcgtt
    33601 ttgacacgcg agtggtcagc gacaaaaaga ccatcgatat ctttgccgcc acccgcccgc
    33661 aggcaaaatg gctgaaatgc gaactgggcg aaccgttgtt tcgcatcagc aaaatcgcct
    33721 ttgatcagaa tgacaaaccg gtgcacgtct ccgaactctt ctgccgcgcc aatcgcatca
    33781 ccttaactat tgataataaa agacattaac cgtaggccgg ataagatgcg ccagcatcgc
    33841 atccggcgat gctggcgcgt tgaattttac atcccgtacg ttcccctcac cctaaccctc
    33901 tccccaaagg ggcgagggga ccgttcagta cggaatttag cttgacggtg ctatccagca
    33961 ttaaatactg gcaatactct cacgcaaaat ccgcagcacc gtctcttcac cgctgcgatt
    34021 cgggttaatg cgaatcgcac aatgttcgag ttgtggattc gcctggcgaa acgttccgga
    34081 aaggcgataa aagagcggcg ggatttcata tttcgactct gcacccaccg ggtaaggcaa
    34141 ggcaccgcgc ttttgcgcct cttccagcac tctggcggca atcggctgat gaaactcgac
    34201 aatcaacacc ttcgactgcg cattagcaat caccgcgctt ttcacttccg gcaccgcacc
    34261 accgttaagc aaagccagca accgttcaga tacccctgcc tgcaccgcgt gcatcactgg
    34321 cgcaaacacc agaccacgca atacttccag cgcctgtgcg ccctggatct ggctaccgcc
    34381 ggagtaaagc gtggcgcgaa tacggtggat aacatcagca tcgccgacca ctgcaccaac
    34441 accctctggc ccaaatagct tgaagcagga aaatgtcgag acattcgcgc cgcattcaca
    34501 gccgattcgc gccaccttca tcaccgcata gttgtcatcg gttaacgctg gaacacctgc
    34561 cgcgcgcaac gttgccagca catctgccag cacgtagctg tcctgcggct gctggcgcgt
    34621 atgctgcacc agcgccgcat ccggttgttg ctcgtcgacg acctgcttca gtgccgacag
    34681 gtcattgaaa tcaacagtaa taagcgtcag ccccatctgc tcaataataa cccgtgtcgt
    34741 cgggtaaaca ggcgcgtcat gcaccagaag acgctgcccc ggtttgagca aagccgccag
    34801 cccggcgcga atcgcgccag tccccgcgcc ctgcaccagc gccgcagcct gtgcgtgaaa
    34861 tgcatcagcc agcacctgtt ccacacgctg ggtaacgcgc ggttgattca gtcctggcgt
    34921 taagcctaaa tcaccgccag taagaaactc gctgccgggg aaatggcgac aaatgctatc
    34981 caccagcgca aactgctttt gctgcgcctc aataatcgtc aggctttgca gaggaaacgt
    35041 cttcataggt gccttcctta agccggaacg aacaggccaa gccagtaaag aacattcagc
    35101 agaataccgg tgatcattac tgccacgacc ggtgccgcca ttttctgtac cggacggccc
    35161 agggattcgt tgaggaagta aatggcaacc gcgatagaga atccggtata actcgccatc
    35221 ttaattgctg cgaaaatcga accgaccagc agcgccactt ccatcagcat attcatggcg
    35281 ttacggatgt tatccgacgc attacgcacc gacgggtagc gtcccagcca tttgccgatc
    35341 gaacgaagca gcaagacttc cgccgaaata actactgcgc ctaataccgc tgcaaccatc
    35401 ggattcggcg agagatagcc caccgcataa acaaaggtaa agcccgcaac tgcatacaca
    35461 cccgttgcta aggcggtggt ggcaatcaac ggtacaaaac ccagcccgcg cataaattct
    35521 gccagtgccg cctggttgat cagcgtttgc gattgttccg gcgttacgcc tgcggagtac
    35581 gctttctcca gagtgaagat cgacacttca ctgccggcga aaatcttcat gctggcaacg
    35641 gcggcaatca atgctcccac gatggcgata tagggtaagt ttttgataat ccgcgacgtg
    35701 cgttcttcaa acaccgaaag cccgctggca tcaatatcat tttcatcacg atgacgcagg
    35761 tcgtgagtta tcgcgatccc cagcagcatc accatgccaa taaagatttc gatggattca
    35821 gggttaagat gtgggaaata gcgcacgact actacacggg tcatcagaac aaccacggcg
    35881 gcgaccagac tttgcttcca gccaaactgg tagaaaatcg ccaccagcgg gaacagtgca
    35941 aaagcagaaa ccaccggcga gcttaattcc ccgaggctac ctaatacatc caccggcagc
    36001 gcggtcagca gctggtttac tggcaacagg caagtaagga tcaacacgcc ccagatagcg
    36061 ccaagaccaa acgccatcag gctgtttatc gccagtacgc cgaggatatc ggtaggaaga
    36121 aagagtaacc atgcgttgag cagcccggtt ttcagggtaa aagagatccc caccgaggcc
    36181 acaaaaccaa tgctcagacc aaaagcgata ctccccgcct cgcgacggtt catatagcct
    36241 tcaatcagtt gcggcaggag cgggcggatg ccgtcatgaa aaacagccgc cgagcgatgc
    36301 gccagaagcg atgtcatacc cgccaggcac gccaccacga taatctgaat atacagatcc
    36361 atagtcgtgc cttttatttc agatggttaa tcagcatcgg aatcgcgtgt tcgacgtgct
    36421 caacggaaag gccaaacgcc acttttcctt cagcgatcat tttggcgata tgctcgtctt
    36481 tcgctttaat gcctggtttg gcaatggtgc aacttttgtt atagccaatc accgcgatgg
    36541 caatcgacaa tgccgcgccc gcaccggtat tacacgcacc gatgtagtaa tccagttgcc
    36601 cggatttcac tttcattgcc gcttccatgt cgttgtgaat gaaaacttca aaactgccag
    36661 gagccgtcgc ttcaatggtt tttttaatct gctcacgctg taagcctgca acgccaatct
    36721 ttttcatgag tctgtcctta ttggaaaaat tgagagggag tttcacgtaa catcacatcc
    36781 acatcggcct gactgaatcc tgactggcgc aattgcggaa taaaggtggt caataaatag
    36841 tcatagccat aaccaccatt ggcttttaaa tgggagcggc gcgtaatatc catcgacagc
    36901 atgacgcggt tcagcaaccc acggtcacgt agcgcgtgga gcatcgcaat gcgcttttcg
    36961 tccgggtagt aactgttctt accaatggtg tcgaactgca cgtacgcgcc gagatcgatc
    37021 atcttcaaaa tgttgtcgag gttgtctttc agatcgcagt gaccaacggt gacgcgcgaa
    37081 agatcaactc cgtgggcttg caacagagcc agttgttcca gccccatggt gctgaacgac
    37141 gtatgcgtgg agatcgggcg tccggtctgg ttatgcgcca gcgcagcggc aataaatacc
    37201 ttctcttcca gcggcgtaat ctttccttcg ctggagccga tctccgcgat gatcccggct
    37261 ttcagctccg tgccatcgat accctgttca atttcatcga ccatctcctg cgccagttcc
    37321 tgcacgctgc gggtcaccac atgttccggg aaaaacgcat cctggtaata accagtacag
    37381 gccaccacgt tgatcccggt ttcgcgcatt acatcgagca taaattgcgc attgcgcccc
    37441 atgtaacggt tggtcatctc aatcacatta cgtacacccc gggtcatcag gtcgttcatc
    37501 tcctggcaaa tgaacgcata ctgatcaagg cggcagtcca cgttgttttt aaagccggag
    37561 agatcaatat gcagatgctc atgggccagg gtgtaacccg tcggatcaaa actcataggg
    37621 tgtctcctgc aaaccggagg gaggaaagaa aagagcgacc attttgtggc ggtggcgcac
    37681 ggaaaaattc acacacggta gctcccacat cagaaagcgt ggtgcgcaca ccgagctgcg
    37741 tagcagccag gccttgctga taaaccagca gcggcaccac ttcgcgggta tggtggctgt
    37801 gaccaatggt cggatcgttg ccgtgatccg ccatcacgac caggcaatca tctggctgca
    37861 tcgcatcaac aagccgggca aggttacggt caacgacctg caaacgttcg gcataacgtg
    37921 cgacgtcttc tgcatgacca gcgaggtcgg tttcctggat attggtgcaa ataaacgccg
    37981 tcggatgggt gttaaattcg tcgagggtga tatccataat ccgctggcta tccaccaggt
    38041 tttgccagct cacgccataa ggattgctga cgatatctgc caccttaccc accagcacgg
    38101 ttggcacgcc cgcctcatac agtttttgcg gcacctgcac ttttttatcg acaccgtagc
    38161 ccatatgcac gacctagaag ccgttgtcat aagcgccaga acgcggcgca ttgataccaa
    38221 taaagcgccc ttctttgctt tctgcggcat cgagaatgcg ttgactgtcg gttaacaggc
    38281 caccaaatgt aatgacccga ccgacctgta cctgctcacg cacgatacga ccaattttga
    38341 ttgcgtcgtc aaaagagatc acagagagat tggcggtaat gttatagact tggcctaaat
    38401 ccgcctcgag attatcgcca atcgcaaccg cctgattgac ccacagaaat tgcagttcat
    38461 cgccacggcg ctccacctgc caaccggcgg atactaatgc ctactcaaca cggtcaatca
    38521 catcgcaaaa aggcatccgc agcggcggta acgggcgcgt gcctaaaatt tcctgatgcc
    38581 ccataaaggt atcgccacct tcatgttgca gctctgccac gccccaggtt gcggaatctg
    38641 acggctgcat atcgcctggc gcataaccca atgcgttgat tagccccagc ttctccagcg
    38701 ttggtagctg caaatgcggc aactggctca ggatgtgacc acatgtattc gctcccgcat
    38761 cttgcggacg caccagcgtg acatctttca ttgcccctac gccaaagcta tcaatcacta
    38821 acaccacaaa tcgcgccatt acgcccccag agagtttcca agactgtcat aacgaccaac
    38881 gattttcggt tcgccacggt gaataccgga caccagcacc acatcgctac gggtgacaaa
    38941 aatctgcgtg cggaaacaga gcaccactgc actgcttacc ggaaactcgc ctgccagcga
    39001 cagggtgtag tcgatactgc tgtcatccac agttttgaga ttggtttcag taatcttttg
    39061 attttctggc gtaaacacca acgcatgttg cgcatgacca cgacggtagt aaccgccgcc
    39121 gtagcagtag ctgtcgccac ggaaatgatg ggagatttca ctcaaccaga gcatcgcgat
    39181 acgttcagac tgatcgccct gctggtttgc cggaatagtg cccgtcagcg catgaccggg
    39241 ttcggcatga gtcacaccgt attgcgccag taatggcagc gaagtgcagc tggtcgctga
    39301 aggcgcgttc agttgctcaa gtgcaatacc agatttcgcc agttgatccc gtgcctgtat
    39361 cagcgtgtga agattcggtg tcggcaaaac ttttccggca gcctcatccc aaagcaggca
    39421 agggaaatgg gtaagtccgg ctaaatgcag acccggcagt ttcttaattt cagcaaccac
    39481 ctcgtgcagc gaatgctgta caaaaccgct ctcctgcccc ggataaagaa aatcatcgtc
    39541 gctataaact ttgagcagca caaactgcac acgtccggcc ttcaccgctg ccgcagaaac
    39601 ttcccgcgct ttgtcgagag taaacacggt gatgacgtcg gtgccctgtt caacggcgtc
    39661 agcaacctga tgacaaggga tttgtaccag atgcccctga tgcgccaccg acaaaccagc
    39721 gcggcgcatg actcgcgcct ctttgtaatc caccgccaca atgccgctgt aacctaatgc
    39781 cagcaatttt tccgccagcc atgggttacg gccaaactgc ttggtcatca gatacagttc
    39841 aatgccgtaa agccgcgccg tctcaatcag tcgcttaccg ttttccagta cctgatccac
    39901 gtcgatcacc cagctgtccg gggcgatctt gccctgctgc cacaaactta gtgcggcgga
    39961 aatcagcgcc gggttctggc gtttcaatgc ttctacaaac atcttgttgc ctcgtttctg
    40021 ttccttgaat taaatgttca tttttttgaa tatttaggtt gtgcgacgtg gatttgtcgg
    40081 gagcttcaaa cctcttcgtt agcagccatc cataatccat aaaggttggc cagcaaatag
    40141 ccttcttcac acgggttaac ttccagtgcg aattccttca acaacacctg atgcaattgc
    40201 acaacggctt gccagtggct ggattgcgcc agctctgcca gcaattcgtt atccagcggc
    40261 tctatttctt caccacggcg actgcgcatc agtgcactcg ccatatgtgt catcgccatc
    40321 gttccttgct cactgcgcac cggcagatgg cactctgttt ccagtacgtt gacgacctgc
    40381 atcatgcctt tgcagatgtc cttatcaata acgcctgcct cgcaaagcag gttgagtctg
    40441 gtttccatat cagatgcctt tcgtttaata actcggttcc tgttccccac tcactacccg
    40501 ttgttgatgg gcgagcaggg cgtgtttatc tacaacggca cgcagcagtt gttgcattgg
    40561 gtaatcatcg actcgcgtca ggaccacggc ttcggtggcc tgtaaaaatc gcggatcgtc
    40621 tgtgagcggc gtcgcctcta atcccagcat ggtcagttcg ttttccgcca ccacgttcca
    40681 gataaccgca tcgacatcgc ctttaacaat gcgttgtaaa ctctcgtgat aagagagatc
    40741 gacacgttcc acatcactat cgccaaaaaa gacatcggtc atgattttct gatccgccga
    40801 acgactatcg agacctactc gcttaacatt tccagactca cctttgcggc atatcagttg
    40861 atgttcacca acatacgtat gcgggccaag ctcgagggcg atgcacaaat tgttttggct
    40921 gagataactt tccgccgcca ggcgggaaac tactgccatg tcatagacac cgttaagcaa
    40981 acactccaca cgaatatccg cgccacgcat atgcgcataa taaaaaggaa tgccatcaaa
    41041 ctgggctttc agcccgcttg ctaaaccttc gtacaagcgg gtatagggca aaggcatggc
    41101 acacacaacg ttattgatat ccacatgact cagcagcgct ttgttatcca tttcgaccag
    41161 ataactgcca ttgcgcccac ggcgttcaat ccgtatcgct ccacttgatt ccaacgtttt
    41221 caacgcggtc tgcgtcaggc caaccgatga acggcattca gttgccagct catctatggt
    41281 tttcagtcga ttaccgcatt tttcacccaa caaataacgg gccagcgtcg tgatgacgac
    41341 gccttctttt ttgataaacg ttcgacgcat aatatatttt caataaaatg aatgtttata
    41401 tcttcattaa aatgaagata agtggcaact gtgagatgtg gaaacggcga ggcgcttcac
    41461 attttttcgg attattgcaa atgaggataa aaaaaccggg tttccccggt ttcagagtga
    41521 tgacgaagtg atttattcga caatgcgagc aaagggtaat tcccactggc gcttgcctgc
    41581 tcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgtccgagcg ttttgatatt
    41641 acggcttcgc cacaaaacca atcgcttcgt acaccgcttt cagcgtacgg gaagcgtgta
    41701 cactggcttt ttctgcgccg tctttcatca cctgttgcag gaaggcttca tcgttgcgga
    41761 aacggtgata gcgttcctgc aattcagtca gcataccgga aacggcatca gccacttcac
    41821 ctttcagatg accatacatc ttgccttcga actgtttttc cagttctggg atgctctggc
    41881 ccgttaccgc agaaaggata tccagcaggt tggaaacgcc cgctttgttc tgcacatcgt
    41941 agcgaactac cggcggctcg tcggagtcag tgaccgcgcg tttgattttc ttcactaccg
    42001 atttcggatc ttccagcagg ccgataacgt tattgcggtt atcgtcagac ttggacatct
    42061 tcttggtcgg ctccagcagc gacattacgc gcgcgccaga tttcggaata aacggctccg
    42121 gcaccttaaa gatctcgcca tacagcgcgt tgaaacgctg ggcgatatcg cgactcagtt
    42181 ccaggtgctg tttctggtct tcacccaccg gtaccagatt agtttgatac agcaggatgt
    42241 ccgcagccat cagcaccgga tagtcaaaca gaccagcgtt gatgttctcg gcataacgcg
    42301 cagatttatc tttaaactgg gtcatgcggc tcagttcgcc gaagtaggta tagcagttca
    42361 gtgcccagcc taactgcgca tgttctggta cgtgggactg aacaaaaatg gtgcttttct
    42421 caggatcgat accacaagcc agatacaagg ccagcgtatc cagcgtcgct ttacgcagct
    42481 tctgtgcatc ctggcgcacg gtgatcgcgt gttggtcaac gatacagtaa atgcaatggt
    42541 agtcatcctg catgtttacc cactgacgca gcgcacccat gtagttacca atggtcaatt
    42601 cacctgaggg ctgtgcgcca ctaaaaacga tgggcttagt catttttcga ttcctgattt
    42661 tcgctatgcg gaagccctaa tgcgggcaga aggtcattta tagactgata aattacatca
    42721 ggctggctga gatcgatagc ctcgccgtag ttatatccgt aggttaagcc aactgatggg
    42781 caacctgccg cttttgccgc ctgaatatca ttgcgtgagt cgccgacaaa cagcatctgt
    42841 tgtggggcaa ttcccatccg ctcagccacc agtaacagcg ggtccggatg cggttttttg
    42901 ttttgcacat catcgccgcc gataaccacg ctaaagtatt tggcaatatc taaggcttcg
    42961 agcagcggcg cgacgaacgg cgtcggtttg ttggtgacca ggcctagcgg caggccttta
    43021 gcctgcaacg cgcccaacgt atcggcaacg tgcgggaaca aaaacgtccc ctcttcggca
    43081 acctcgctat agtagcaatc gaacagttta cgcagaatac gtacctgttc ttctgccgga
    43141 atgtcgtcat caacgggcgg tttacccatt gttttacgca gagtcgcacg ttcctgacgc
    43201 gcccaggtca atgcgcgctc catcagaaca tctgcgccgt taccaatcca agtaataacg
    43261 cgttcttcac ctgcgatggg caactccagc gcatacagcg ccatatctac cgcagcagca
    43321 agaccgggag cactatcgac cagcgtacca tcaagatcaa aagcgacgcc gcgaatatct
    43381 tcaaacttat tcatgactta cctttgccag ttcactgcgc atttcatcaa tgactttttt
    43441 gtagtctggc tggtcgaaga ttgccgaacc ggcgacgaac atatccgcgc ccgccgcagc
    43501 gatttcgcca atgttgttca ccttcacgcc accgtccact tccagacgaa tgtcaaagcc
    43561 agactcgtcg atacggcgac gtacttcgcg cagtttatcc agtgtttgag gaatgaaaga
    43621 ctgaccgccg aaaccaggat tgacggacat cagcaggatc acatccagct tatccatcac
    43681 gtaatccaga tagctcagag gtgtcgccgg gttaaatacc agacccgctt tacagccatt
    43741 ttctttaatc agttgcagcg tgcggtcaac atgctcggag gcttctggat gaaaggtaat
    43801 gatgctggca ccagcggcag cgaaatcagg cacaatgcga tcgacgggtt tcaccatcag
    43861 atgtacgtcg ataggggcgg taatgccata gttacgcaag gatttcagca ccattggccc
    43921 aatcgtcaga ttgggaacat agtggttatc catgacgtca aaatgcacga catcagcgcc
    43981 agctgccagg gcttttgcgg tatcttcacc caggcgggca aaatcagccg acagaattga
    44041 gggggcaatc aaatactgtt tcatccgctt ctccttgaga attatttttt cgcgggtgaa
    44101 acgactcctg gtttgtacaa agccagcagt tcgtccacct ttttacgtgt gccgccgttg
    44161 ctgcttatac tgcgtcgaac tttgacgaca tgcaattttg cgcgctgata ccactcacgc
    44221 gttaacatcg tatcgtgatt ggagatcagc actggaatat ggcgctcaac cagaccttcg
    44281 gcgatctccg ccagatgcgc ttgttgttca agcgtaaaac tgtttgtgtg atacgccgta
    44341 aagttggcgg tcgcagacag cggtgcataa ggcggatcgc aatagacgac ggatgcatca
    44401 tctgcgcgcg ccatgctatc ggcgtaagac tcacaataga aaaaggcatt ctgcgctttt
    44461 tcagcgaagt gatacaactc tgcttccggg aaatagggtt ttttgtagcg gccaaacggc
    44521 acgttaaact caccgcgcag attgtaacga cacaggccgt tgtaaccgta gcggttcaaa
    44581 tataaaaaca gtaccgcccg acggaacgga tcctggcttt tgttgaactc ttcgcggaac
    44641 tgatagtaaa cctcggcgca atttgtttcg ggaacaaaca gctcgcgtgc ggcctgtacg
    44701 tactcatcag tacgcatctt cacaatgtta tagagactga tcaggtcgct attgatatcg
    44761 gcaaggatat aacgagaaaa gtcggtgttg agaaacaccg acccggcacc tacaaaaggc
    44821 tcaaccagac attcgccctt gggcaaatgc cgtttaatat catcaagcag gggatacttg
    44881 ccccctgccc acttcaaaaa agcgcgattt ttcttcatgc tgactaacta attacacctt
    44941 ctccggctgt ggagaaagct ccgacagcat ccagcgcttc aggcagctcc cgcaacgata
    45001 aaccattgcg ggagataacc ttgattactt cagatcggcc tgtacctgac gcagcggttt
    45061 cgcccacggg tttttggcct gaacatctgc tggcaatgta gataccgctt ttttcgcctc
    45121 ttctttcgaa gcgtacacgc cagaaaccag gacataccac ggctgaccat tacgcgtcgt
    45181 ttcatagaca acgtagtttt tcagattctc tttcttcgcc caaccgttca ggttgtcgta
    45241 gttagaggaa ctgctcagct gcagagtgta atggctggac ggtgccgatt tcaacgaacc
    45301 aacattacct gcggtcttcc ctccagcggc tggtgttgcc gtggtttgcg ccggggatgc
    45361 cgtctgtact ggagccgtag tcgccgtctc ttttggtgct ggcgtagaag tcgcagccgg
    45421 tgccttcgtg ctcgccacag gggcagctgg ttcagtacgc ttcggcgtct gtgctaccgg
    45481 cttcggctcc gttttcacgg ttgcttgcgg ttttttcggt tcaatcaccg cctgctgacg
    45541 agctgggcgc gtagtggacg gacgttcagc ggtttgcgtt ttcgccgtgt cacgcgatgc
    45601 attgccattg cgaacaggcg cgacagtcgc gggttcggtc ggcaatgtag aattgaccgc
    45661 cacattgttc aactgttgct gattttgtgg ctgggtcagc gcattgttca ggtcaccctg
    45721 cacttcaaca cgttgttgac catccgttgc cgccggggtt tgcccttgag tcggcgtaga
    45781 agagatcggt ggcagagaaa catcctgctg agtattttcc gcagacgtgg ttcccggcgc
    45841 tggctgcacc ccattcgcct gatcggtcgc attgcctgca agatcaatac tcttctcgcc
    45901 agacgcggtt tgatcgctgg aagaggtcga gggggctttt agcgcagaac cgataccgat
    45961 gatcaacagc agtagaacca gaatgccgac gcccatcatc atatactgac gagaagcggg
    46021 tttactggct gcttttttgc gcttacgcgg acgacgctct acgcgctctt catccacggt
    46081 ttcatcttcg gattcgtcaa tttcttcttc gatttccggt tcctcattgc gctctttttg
    46141 cgcacgagtc ggacggcgat cgtcagtgtc atcaagttca atatcatcaa aattgatctg
    46201 cggttcgcca cgttcagtac gctcagaacg ttcagaagat tgacgagaac gaccagtacg
    46261 acgatcgctg ggatcgggtt tcagctcgtc ttctggtttg aattcatcca tttaacaccc
    46321 cactaaaagg ttaatgctta ccacgttgca attaacctga agctaataga ccgcttgata
    46381 agcggcctga cctttcttgt tgttacgctg attgacaatc ggcaatggcg ttaagaacaa
    46441 gctcgtgcga aacgccgctg cgaacttcac tcttaccaat tgccaacgga agaattaagc
    46501 gcatctctcc cgcaaggact ttcttgtcac gcagcatatg cggtaaatac gcctgcgcgg
    46561 acatttcgcg cggcccattg accggtaacc cagcccgctt gagcagggtt ataatacgct
    46621 gcgtttcggc agaactaaac tgcccgagac gttccgacgt ccgcgccgcc atcaccatac
    46681 ccgcagcgac cgcttcacca tgtaaccaat tgccataccc catttcagct tcaatggcat
    46741 gaccaaaggt gtgtcccaga ttcagtaaag cacgtaaccc ggtttcgcgc tcgtcggcgg
    46801 cgacaacttc tgccttcagt tcacaacaac ggcgaataca gtacgccatt gccggaccgt
    46861 ccagacgcaa caacgcatcc agattctctt ccagccagtt aaaaaacgca ccgtcaagaa
    46921 taatgccgta tttgatgact tctgccagcc ccgacgctaa ctcacgcggg ggaagcgttt
    46981 tcagacagtc gagatccacc accactgaag caggctggta gaacgcgcca atcatgtttt
    47041 taccgagggg atggttgacc gcagttttgc cgccaacgga ggaatcgacc tgcgacagta
    47101 acgtcgtcgg gacttgaatg aaacgtacac cgcgctgata actcgccgcc gcgaagccgg
    47161 tcagatcgcc cactacgccg ccgccaagcg ccaccagcgt agtatcgcga ccatgcggtt
    47221 tttgtaacaa cgccgtaaag acggtatcga gtacagccag gcttttatac tgctcgccgt
    47281 cagggaggat aacgctatcg acattaacac ccgcctgttc aagtacgccg cggaccttat
    47341 cgagatacag aggagccagg gtttcgttgg tgaccaacat gacctgctcg cccgatttca
    47401 gcggtaagaa tgaagctggt tcattaaaca aaccagatgc gatggtaatt gggtaactac
    47461 gttccccgag agtaacgaca atcctctcca taacgcgaca tccaccttaa ttactgtacc
    47521 cgcagacgag tgtatataaa gccagaatta gttgctttcc agcatgtgaa taatctggtt
    47581 tgcaaccact ttagcgcttt gatcatcagt acgaatggtc acgtcggcaa tctcttcata
    47641 cagcggattg cgttcattgg ccaacgcttc cagaacttca cgcggcggtg tttcaacgtg
    47701 cagcaacggg cgttttttat cacgctgcgt gcgtgcaagt tgcttttcga tggtcgtttc
    47761 aagataaacg acaacgccac gagcggaaag acggttacgc gtttcacggg atttcacaga
    47821 gccgccgcca gtagccagca caataccctg tttctcggtc aactcattga tgaccttttc
    47881 ttcgcgatcg cggaagcctt cttcgccttc taaatcgaaa acccagccca catcagctcc
    47941 ggttcgtttc tcaatctctt gatcggaatc gtaaaattcc atattgagtt gttgagctaa
    48001 ctggcgccca atagtgcttt ttccggcacc cataggccca accagaaaga tattgcgttt
    48061 ctctgccatt ttttcggtac tactaagact attcgttaat gataaacccg cttcgctcag
    48121 agagcgccgc aggacatgaa ctgaaacctc ataagatatt gcgagagtca gactgaaaat
    48181 tatctcaata ctccagcggg tttggcaact gaataaatca cccatccctc tgcatatctg
    48241 gtcgctgcaa gcgcgctgcc ttgctaccac cgctctggcg ataaatcacc gggtaagatt
    48301 agcgtaaaaa agacagcaaa atgccgcctg aatgataaat catcatcatg gaacgcaggc
    48361 agcgaagagc accaactctc aaatcggtac tccttgtatg ctaaatacct gcgcgcgtca
    48421 aatagatgaa acacgttcag cataaaaaca ttaccgcttt tacggctgtt tactcactgg
    48481 aaaccagtcg tggcgtgata aacaccacta actcgcgtcg ttcatcttct tttccgtcat
    48541 gacgaaataa ttgcccgaac cagggaatgt cgccaagcaa cggtacgcta tcctgacccg
    48601 atttattttt acgggtaaaa atgccgccca gcgccaacgt ttctccgctt ttgacctcga
    48661 cctgcgtttc gatctcctgc ttatcaatcg ccagcacttc gccatcggcc tgctgtagca
    48721 cctgccccgg aacgttctgg ctgatgtgta atttcagccg gatgcgacct ttttgtaaca
    48781 ccgtgggcgt gacctccatc cccaggacgg cctctttaaa ttccaccgac gtcgcgccac
    48841 tttccccgct ggaaacctga tatggaattt cgctcccctg tttaatgctg gcaggctgaa
    48901 gatgtgaggc cagcagacgc ggactggcga taatatccag ctgctgtttt tgttcgagcg
    48961 cggaaagctc aagatccagc aagcgtccgt tgatgcgccc aatgttaaaa ccgacatgcg
    49021 ttgtcgccgt cgctacggag aggtcgctac caagcgtggt gacttgccca acgccaccag
    49081 cgtgttgcgc atcggccagc gtccatttca cgcctaactc acgcaaactt ttttcattaa
    49141 tggtgacaat atgcgccgac agctcaacct gcccgaccgg cagatccatt tgcgctaccc
    49201 actgttcaag cgcgcttaac gccgttttgt tatctcgtag caaaaggcga ttggtgcgtt
    49261 tatcgacggt catactccct ttggcactca gtagcttctc ccccgctttc gccagttctc
    49321 ccgcgtcggc gtattgcagg gttatactgc gattttccag cggcagattt gcctgcgccc
    49381 gcgcctgctc cgcctcctgg cgggcgatat tgttattctg ccaggcaatg gaatgcactg
    49441 agagaatgtt gccttcctgc cgcgttatca gtccggcgct tttcactaca gtttgtagtg
    49501 cctgcttcca gggaacatct gttagatgta acgacaccgt accgctgacg tctggcgaca
    49561 cgaccaggtt caacttctcc tgttcagcca gcgcctgcaa cacctgagct accggaacgt
    49621 catccaccat cagcgtcact ttttgcggct ttgccgcctg tacgccgggt atcagcatca
    49681 acagtagtgc ggctatccat tgcttcattt gtatctcctt gccgttgcca caaccattgt
    49741 ggcggttcgc agtttgtccc ggtacccagc gttagtacgt ctggcgtcag ctgtaaaatt
    49801 gtccagccgt tttccagcac atcgttttgc tgcacccgtc gccatttctt ttgcccgtct
    49861 tttattacac cgatgatgcg ctcgcctcgc cctaccatcc cctgatagcg ccactggcta
    49921 agttcgctaa tccggcatag atcttccggc ggtttaaaag ggtcacgcat accggttaaa
    49981 aggcacaatg caatacctgc caacaaccag cgtttaaccc tcatgcggcg tctccagttg
    50041 tagcgtgaac aaaagatcat caccttccac gcttaacgaa aaacggctca cgctgacgtt
    50101 gcgctctgcc agccgtgtaa atgccgacgg cactgcttcc cacagcgttt tcaacgccaa
    50161 ctcgcctccc tgcgcggatg gatgccagga aaccagttgt gcgccgggca actgaaaagc
    50221 cagtggcgaa aagggcagcg ttttttcctc gctgaaggga gtggtgtcta ccaggcgata
    50281 cagtgcggcc cactgctggt gatgacttgc ccgtagtcgc gtcgcgctct tcctggtgtg
    50341 tcgatgacag aaaaatgagc gtaactaaca tcagcaacca gactgcccag caaagctggc
    50401 ggaggcgggg tgatgtggcg aaccaccagt caaagaacat gttcatcgct aaccttcctt
    50461 gttaactgat actcaaattg ccagcgtccc tgcgcatcct gctgcgtggc tccccgctga
    50521 ttgagatgaa aagacgcatc ctggcggagt gacgtttcta gtgcgtttaa cgcggtaatg
    50581 cttgttgtca gccccttgat ctccagcgtt ccctgctgcc agcttatcgt tgtcagccag
    50641 gcgtgctcgg gtaaaagcgc cgccagtgct tccagcgcag attgccagtc gcgggtaaat
    50701 tgtcgctggc gctgccgttg cgaacgctgt tcgcgtaact gctgttgctc cagcaaacgt
    50761 ggcttcgtta tctgtaagct gcgggcgagt tgttgttccg cctgaagcaa aactgcgtct
    50821 atgcgcgctt cggcgctgcc tgtctgacgc agtattagcg ttatcccgac ggccagcagc
    50881 agaggcgcaa cgaacatcag caaccagaaa cgcagaaaag cggtccggcg ttgctgtcgc
    50941 cagggcaaaa aattaattga cgggttcatc agtacgcctt cccaagcgcc agccccagcg
    51001 cgatggcaaa gtctccacca ggcggcggta gcggcggctg acgaacagaa acggcctccc
    51061 aggggtcaaa tccgccttcg ccacatatcg cgacgctgtc gggatcaacg gataacgctg
    51121 ccgccagctc cttcgcgcta gtcatcccca ccgccagttt gcgcccccag ctatagcgcg
    51181 tcgcccacag ccactgttcg ttatcacgcc aggccagaca ttgctgatga gaaggtaaaa
    51241 aaggcaggaa tcgctgtaat gcactggcat ccggggtgat cgcgctcaca tgaacacgca
    51301 acctttctgc cagcgtaagc agcgttgcca gctctttgct ttgcgcggca gtcacgttat
    51361 aagcggggct gagtgagtct tcgctgtaat cgaagcgcag ggagtccgga tccatatcca
    51421 gctcgcgggc catcgtccct gacagccagg ccgtttgctc ccgctcacca agggacatcg
    51481 acgggcgcgg aaatgaccgc tgtaatgtgc gactggcggg aaacgccaac ataatgtgat
    51541 gacgctgcgg cagttcgcga ctccacggta acaacgtttt agccagctgc tgcgcatcaa
    51601 caatccgccc atctttgata atgtcgtgcg ccagcggcaa ccgccaccag cgttgcaaaa
    51661 agcattcttt tgcgccccgc acgatcgcaa ccgctgccgc ttcttgctgt tgtaaatgca
    51721 aaccaatttg ccagatctta aatgccattg tgatgatctc cttatcaccc gtcactctga
    51781 cgggtatatc aatgcgtctg gcttgccttt atactaccgc gcgtttgttt ataaactgcc
    51841 caaatgaaac taaatgggaa atttccagtg aagttcgtaa agtatttttt gatccttgca
    51901 gtctgttgca ttctgctggg agcaggctcg atttatggcc tataccgcta catcgagcca
    51961 caactgccgg atgtggcgac attaaaagat gttcgcctgc aaattccgat gcagatttac
    52021 agcgccgatg gcgagctgat tgctcaatac ggtgagaaac gtcgtattcc ggttacgttg
    52081 gatcaaatcc cgccggagat ggtgaaagcc tttatcgcga cggaagacag ccgcttctac
    52141 gagcatcacg gcgttgaccc ggtggggatc ttccgtgcgg caagtgtggc gctgttctcc
    52201 ggccacgcgt cacaaggggc gagtaccatt acccagcagc tggcgagaaa cttcttcctc
    52261 agtccagaac gcacgctgat gcgtaagatt aaggaagtct tcctcgcgat tcgcattgaa
    52321 cagttgctga cgaaagacga gatcctcgag ctttatctga acaagattta cctcggttac
    52381 cgcgcctatg gtgtcggtgc tgcggcacaa gtctatttcg gtaaaacggt cgaccaactg
    52441 acgctgaacg aaatggctgt gatcgccggg ctgccgaaag cgccttccac ctttaatccg
    52501 ctctactcaa tggatcgtgc cgtcgcgcgg cgtaacgtcg tactgtcgcg gatgctggat
    52561 gaagggtata tcacccaaca acagttcgat cagacacgca ctgaggcgat taacgctaac
    52621 tatcacgcac cggagattgc tttctctgcg ccgtacctga gcgaaatggt gcgccaggag
    52681 atgtataacc gttatggcga aagtgcctat gaagacggtt atcgcattta caccaccatc
    52741 acccgcaaag tgcagcaggc cgcacagcag gcggtacgta ataacgtgct ggactacgac
    52801 atgcgccacg gctatcgcgg cccggcaaat gtgctgtgga aagtgggcga gtcggcgtgg
    52861 gataacaaca agattaccga tacgctgaag gcgctgccaa cctatggtcc gctgctgcct
    52921 gccgcagtca ccagcgccaa tcctcagcaa gcgacggcga tgctggcgga cgggtcgacc
    52981 gtcgcattga gtatggaagg cgttcgctgg gcgcgtcctt accgttcgga tactcagcaa
    53041 ggaccgacgc cgcgtaaagt gaccgatgtt ctgcaaacgg gtcagcaaat ctgggttcgt
    53101 caggttggcg atgcatggtg gctggcacaa gtgccggaag tgaactcggc gctggtgtcg
    53161 atcaatccgc aaaacggtgc cgttatggcg ctggtcggtg gctttgattt caatcagagc
    53221 aagtttaacc gcgccaccca ggcactgcgt caggtgggtt ccaacatcaa accgttcctc
    53281 tacaccgcgg cgatggataa aggtctgacg ctggcaagta tgttgaacga tgtgccaatt
    53341 tctcgctggg atgcaggtgc cggttctgac tggcagccga agaactcacc accgcagtat
    53401 gctggtccaa ttcgcttacg tcaggggctg ggtcagtcga aaaacgtggt gatggtacgc
    53461 gcaatgcggg cgatgggcgt cgactacgct gcagaatatc tgcaacgctt cggcttcccg
    53521 gcgcaaaaca ttgtccacac cgaatcgctg gcgctgggtt cagcgtcctt caccccaatg
    53581 caggtggcgc gcggctacgc ggtcatggcg aacggtggct tcctggtgga cccgtggttt
    53641 atcagcaaaa ttgaaaacga tcagggcggc gtgattttcg aagcgaaacc gaaagtagcc
    53701 tgcccggaat gcgatattcc ggtgatttac ggtgatacgc agaaatcgaa cgtgctggaa
    53761 aataacgatg ttgaagatgt cgctatctcc cgcgagcagc agaatgtttc tgtaccaatg
    53821 ccgcagctgg agcaggcaaa tcaggcgtta gtggcgaaga ctggcgcgca ggagtacgca
    53881 ccgcacgtca tcaacactcc gctggcattc ctgattaaga gtgctttgaa caccaatatc
    53941 tttggtgaac caggctggca gggtaccggc tggcgtgcag gtcgtgattt gcagcgtcgc
    54001 gatatcggcg ggaaaaccgg taccactaac agttcgaaag atgcgtggtt ctcgggttac
    54061 ggtccgggcg ttgtgacctc ggtctggatt ggctttgatg atcaccgtcg taatctcggt
    54121 catacaacgg cttccggagc gattaaagat cagatctcag gttacgaagg cggtgccaag
    54181 agtgcccagc ctgcatggga cgcttatatg aaagccgttc ttgaaggtgt gccggagcag
    54241 ccgctgacgc cgcctccggg tattgtgacg gtgaatatcg atcgcagcac cgggcagtta
    54301 gctaatggtg gcaacagccg cgaagagtat ttcatcgaag gtacgcagcc gacacaacag
    54361 gcggtgcacg aggtgggaac gaccattatc gataatggtg aggcacagga attgttctga
    54421 ttaaaaaggc gcttcggcgc cttttcagtt ggctgacaaa gtgcatttgt ttatgccgga
    54481 tgcgggtgaa cgcgttatcc ggctaacaaa atcgtgaaaa atcaatatat tgcaggaacc
    54541 ccataagcct gataagcgtt gtgcatcagg caaacttcac gcatttacac tcgcccctgc
    54601 cctttcaacc attcgcgcac gaggaacagc gcgctgacat tacgcgcttc gttgaagtca
    54661 gggtcttcca gcaaatccat catatgcgcc agcggccagc gcacctgtgg tagcggctct
    54721 ggctcatcgc cttccagcga ttccgggtag agatcttgcg cgaccacgat attcattttg
    54781 ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca
    54841 aatccaacct cttcttttag ctcgcggtta gcggcttcgt agacgctttc acccggatca
    54901 attaatcctt tcgaaaaacc taattcgtag gattcagttc ccactgcgta ttcgcggatc
    54961 aggatcaggt gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc
    55021 attcgttcat aaacacgccg cacgccattg ctgaactcca gatccacgct ctcgacggta
    55081 aacagtcggg aacgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg
    55141 ctcatcgtgg gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc
    55201 ggcaatgtga attgcatgtt atttacattt atgtaactta ataaataatc gtcctcaaat
    55261 caaattaaaa gtcaataggt tgaaataact ccaggaattt gctgatattc cgccttcaga
    55321 gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taaactccac
    55381 gcttgccgat agccaaccgc agaatcatgt attgtgtctg gtgcgactga ccacgcctga
    55441 cagactaagt aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg
    55501 gcctgctcac tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgtcc
    55561 tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga acgtagcgcc
    55621 gttgaaaatt atcttgagag cctgacgcag gtattacagg tgcctggccc aacgggagct
    55681 agcgcggcac cgatctctct ggcgctgaat gccgaaagca acaacgtcat gatgctgaca
    55741 cacgctatca cgcgttacgg catctctacc gacgatccga ataaatggcg ttactacctc
    55801 gattcggtag aagtccacct gccccctttc tgggaacaat acatcaacga tgagaatacc
    55861 gttgaactga tttataccga ttcgctgccg ctggttattt cactcaacgg tcatacgctg
    55921 caggagtaca tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc cacgcaggcg
    55981 tcgattcgcg gggaagagag tgagcaaatc gagctactca atattcgcaa agaaacgcat
    56041 gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat cgtcgcctcc
    56101 ttcctgatgt tctttttctg cctgattacc ccggatgtat ttgttccgtg gctggcaggc
    56161 ggcgcattac tgctgctggg cgcaggtctg tgggggctat tcgcgccccc ggcaaaatcc
    56221 tccctgcgag aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa
    56281 aacgatcagg aacagatcaa caatatttca ctcggtatta tcgacctggt ctatcccgca
    56341 cactggcagc catacattgc tcaggatctc ggtcaacaaa ccgatattga tatctatctc
    56401 gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt aaaaaacttt
    56461 ccgttacagc actggctgcg cagtacgatt atcgctgcgg gctcgctgct ggtgctgttt
    56521 atgctgttat tctggatccc gctggatatg ccgctgaaat tcactctctc atggatgaaa
    56581 ggcgcgcaga ccattgaagc caccagcgta aaacaactgg ctgacgctgg cgtgcgggta
    56641 ggcgatacat tgcgtattag cggtacggga atgtgtaata ttcgcacttc cggaacctgg
    56701 agcgcgaaaa ccaattcacc ttttttaccg tttgactgct cgcagatcat ctggaatgac
    56761 gcccgctcat taccattacc agaatctgaa ctggtcaaca aagcgacggc attgactgaa
    56821 gcagttaatc gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag tgcctcatta
    56881 cgttcagcaa ttcaaaaatc cggcatggta ttgcttgatg attttggcga cattgtactg
    56941 aagacagcgg atttatgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc
    57001 aatctcggca acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc cgggaagctc
    57061 gatggcgtga atgtgttatt acgcccggtg agtgcggaat cgctggataa cctggtggca
    57121 acctccaccg cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt
    57181 ccggcccccg gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc
    57241 tggccttcgg catcacttta cgactacccg ccgcaagaac agtggaacgc tttccagaaa
    57301 ctggcacaaa tgctgatgca tacgccgttt aacgccgaag gtatcgtcac aaaaatcttc
    57361 actgacgcca atggtacgca gcatattggc cttcatccga tcccggatcg ttccggcctg
    57421 tggcgctatc tcagcaccac attgctgcta ctgacgatgc tgggtagcgc catttacaat
    57481 ggcgtacagg cctggcgtcg ttaccagcgt catcgcactc gcatgatgga gattcaggcc
    57541 tattatgaaa gctgcctgaa cccgcaactg atcacccctt cagaaagcct tatcgaataa
    57601 cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttc
    57661 cctcatgcat atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg
    57721 cacgttgctc gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg
    57781 gggcgcgaaa aacggggtta cgccacagga agcgatggaa tatatgcgcc agcaatatca
    57841 cgacgtacag catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct
    57901 ggatatctgt gcgatgacca ccgagatggg accgcgcgcc gtactgcgtg aagataccat
    57961 tccgtttctt gaggcactga aagccagcgg taagcagcga attttgctca ccaatgctca
    58021 tccgcacaac ctggcggtaa aacttgagca tacgggtctg gacgcacacc ttgatttatt
    58081 actttccacc cacacatttg gttatccgaa agaggatcag cggttatggc atgcggtggc
    58141 cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct
    58201 cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgattccgg
    58261 gattgccgag aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc
    58321 ctcgctaatg tgaaggagac gccatgaaag aaaaacctgc tgttgaggtt cgactggata
    58381 aatggctatg ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattgaag
    58441 gcggtaaggt gcattacaac gggcagcgca gcaagccgag caaaatcgtc gagctgaatg
    58501 ccacgctcac tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg
    58561 aacagcgtcg ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag
    58621 agaaacgcga aaaaatggcg atggcacgta aacttaatgc cttaaccatg ccgcacccgg
    58681 accgacgccc ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg
    58741 aataactgtc acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc
    58801 tgtttgaaaa ttttgccgtt cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga
    58861 tccttgagaa ccacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg
    58921 cgaccagcct gttaaccgct acgctgaagt ttgatggtga tatcaccgta cagctgcagg
    58981 gcgacggtcc gatgaatctg gcggttatta acggtaacaa taaccagcaa atgcgcggtg
    59041 tggcgcgcgt gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg
    59101 gttacgtggt gatcaccatt accccgagcg aaggcgaacg ctatcagggc gtagttggtc
    59161 tggaaggtga taccctggcg gcctgcctgg aagattactt tatgcgttct gaacagctgc
    59221 cgacgcgcct gtttattcgc accggcgacg tagacggcaa accggctgca ggcggtatgt
    59281 tgttgcaggt aatgcctgcg caaaatgccc agcaggacga ctttgaccac ctggcgacgc
    59341 taaccgaaac catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa gtgttgtggc
    59401 gtttgtatca cgaagaagag gtgacggttt acgatccgca ggatgtggag ttcaaatgca
    59461 cctgctcgcg tgaacgttgc gccgatgcgc tgaaaacgct gcctgatgaa gaagttgata
    59521 gcatcctggc ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc
    59581 tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc gtctccggca gatccgcaag
    59641 ttcattaatg agtatgtccg gcagagaggg ctctgccgga ttttacaact taatggcctg
    59701 atgtgctgcg cttaccagcg cctatgaaat aaatcgcaaa ttattttggt cagatacagc
    59761 gtcgtatccg ataaaagcgt tgtcacttac ttaagtttat ttccagcgtt tcaccatcta
    59821 ccgtcagatc gcgccacgct ttcggcctcg tccccagttt tccatccttc gcagctgtga
    59881 gtaatttttc tttcgttatt tcgttcggaa gtaaactcat atcaagcgcg tcccattctt
    59941 ttctgtctga gtcaaagcca tagacaatga ctctgtcatc attaaaagca aacaggatcc
    60001 gctccgcctg accatcacta ttcagatctt gctcgaccag tacgcaggcg tctttttcaa
    60061 tacaggtcat cacgttatag cgatcctgga ttaaggccga ccagaatgtc gcatccggtt
    60121 taacagaacc aggggcaatt aacacatttt cggctaatac tttttccgat acctgttcct
    60181 gaagatgttg ctctccatct aacgccatca gcagatcccg cgcgcgtttc gggtctttca
    60241 taaacccggc atcgcttttc agcgactcaa gcgccgcacg tccatagcga ccgctctgtt
    60301 cgagcatgta gatagttacc aggtctggcg tgtttttgcc gctctgataa cgcgccatat
    60361 gactgttcac gctaatacgc atactgtcca gcaccggcga attaagaagc accagtatca
    60421 ccaacaccaa taacgaaacc gcaaggttca ctttgccctg aagaacaagg ggattttgcc
    60481 cattacgcca gacgatactg acaaaatacc ccagcgacca caccagtaac accagcaccg
    60541 ccagcgcgcc ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc agccataacg
    60601 cccaggcggc gacgaacaca tacagcggag caaccagcaa agcggtttta atcaggcaac
    60661 gcaacggccc cgtccaggga agtgacgctt tttgcggatc gcggacaata gccattaaga
    60721 tcaattgcaa aaaggccagc gttaacagca acccggcggc ggagatgtgg cgagaaatcg
    60781 cgctcaggcc cgtaaacggc agagtgatga taaacatcag ggttagcaat gatactaacg
    60841 gcagcaaccc cgtggcgatt aacgtgaaca acttttgaat agagtcgatt aaacgtgact
    60901 gtgtgcgcgc gagaatcacc gccagtgcgg taaccagacc taatgtaaga tacataaacc
    60961 agtcggttgc aaaaaagagc gtattgaaaa acttgatgcc aacaagttta aacaactcac
    61021 cccacagtaa aagcaccagc cacgtcaggc cattggcgag aaaaatcacc agtaatataa
    61081 gtacgttatg ccatactgac tggtaaaagt agctatagcg gctactgcca ttgcggatac
    61141 gaaggctttg ttgtatccac ggtagcaaca acattgccat caacagcaga tagcagccca
    61201 aatcccaaat cgccttttca gctctccagg ggttcatgcc atcagtttgc cacttcagcc
    61261 aaccgctcat ccctgtagtg gcaataaaca ccagcgccag ccatccccaa aggcgcttct
    61321 gtttaaaaga gatcacggag aagagtaaaa cggatgaaaa cgcaacggtc gcaggcacgc
    61381 cataaacaat ccaactgtga ttttttccgg caagccaggc gatcaacagg tagcaaacca
    61441 gcccctgaag taatccggta gcaatcatcc cccagcgtgt tgccggtgaa agttcaacgt
    61501 tatccatgtt ggttatccag aatcaaaagg tgggttaatt atcgcatccg ggcagtagta
    61561 ttttgctttt ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat actttaccga
    61621 ttgaatttgc atcaatttca ttaaggaatg cgattccact cacaatattc ccgccatata
    61681 aaccaagatt taaccttttg agaacatttt ccacacctaa aatgctattt ctgcgataat
    61741 agcaaccgtt tcgtgacagg aatcacggag ttttttgtca aatatgaatt tctccagata
    61801 cgtaaatcta tgagccttgt cgcggttaac acccccaaaa agactttact attcaggcaa
    61861 tacatattgg ctaaggagca gtgaaatgcg cgttaacaat ggtttgaccc cgcaagaact
    61921 cgaggcttat ggtatcagtg acgtacatga tatcgtttac aacccaagct acgacctgct
    61981 gtatcaggaa gagctcgatc cgagcctgac aggttatgag cgcggggtgt taactaatct
    62041 gggtgccgtt gccgtcgata ccgggatctt caccggtcgt tcaccaaaag ataagtatat
    62101 cgtccgtgac gataccactc gcgatacttt ctggtgggca gacaaaggca aaggtaagaa
    62161 cgacaacaaa cctctctctc cggaaacctg gcagcatctg aaaggcctgg tgaccaggca
    62221 gctttccggc aaacgtctgt tcgttgtcga cgctttctgt ggtgcgaacc cggatactcg
    62281 tctttccgtc cgtttcatca ccgaagtggc ctggcaggcg cattttgtca aaaacatgtt
    62341 tattcgcccg agcgatgaag agctggcagg tttcaaacca gactttatcg ttatgaacgg
    62401 cgcgaagtgc actaacccgc agtggaaaga acagggtctg aactccgaaa acttcgtggc
    62461 gtttaacctg accgagcgca tgcagctgat tggcggcacc tggtacggcg gcgaaatgaa
    62521 gaaagggatg ttctcgatga tgaactacct gctgccgctg aaaggtatct cttctatgca
    62581 ctgctccgcc aacgttggcg agaaaggcga tgttgcggtg ttcttcggcc tttccggcac
    62641 cggtaaaacc accctttcca ccgacccgaa acgccgcctg attggcgatg acgaacacgg
    62701 ctgggacgat gacggcgtgt ttaatttcga aggcggctgc tacgcaaaaa ctatcaagct
    62761 gtcgaaagaa gcggaacctg aaatctacaa cgctatccgt cgtgatgcgc tgctggaaaa
    62821 cgtcaccgtg cgtgaagatg gcactatcga ctttgatgat ggttcaaaaa ccgagaacac
    62881 ccgcgtttct tatccaatct atcacatcga taacatcgtt aagccggttt ccaaagcggg
    62941 ccacgcgact aaggttatct tcctgactgc tgatgctttc ggcgtgttgc cgccggtttc
    63001 tcgcctgact gccgatcaaa cccagtatca cttcctctct ggcttcaccg ccaaactagc
    63061 cggtactgag cgtggcatca ccgaaccgac gccaaccttc tccgcttgct tcggcgcggc
    63121 gttcctgtcg ctgcacccga ctcagtacgc agaagtgctg gtgaaacgta tgcaggcggc
    63181 gggcgcgcag gcttatctgg ttaacactgg ctggaacggc actggcaaac gtatctcgat
    63241 taaagatacc cgcgccatta tcgacgccat cctcaacggt tcgctggata atgcagaaac
    63301 cttcactctg ccgatgttta acctggcgat cccaaccgaa ctgccgggcg tagacacgaa
    63361 gattctcgat ccgcgtaaca cctacgcttc tccggaacag tggcaggaaa aagccgaaac
    63421 cctggcgaaa ctgtttatcg acaacttcga taaatacacc gacacccctg cgggtgccgc
    63481 gctggtagcg gctggtccga aactgtaatg atttgaagct ggagaatatc tatccagtat
    63541 cttatagaaa gcaaaacggg aggcaccttc gcctcccgtt tatttaccct tcttttgtcg
    63601 tgccctgcgc ccgcgttacc ggcactggca gccaggcgcg aatggaaagc ccgccccgct
    63661 cgctggtgcc aagctccagc atcccgttat ggttatccac gatacgctgc acaattgcca
    63721 gccctaatcc cgtgccgcta atggtgcgcg cactgtcgcc gcggacaaac ggctggaaca
    63781 ggtgcttacg ttgttccggc gcaattcccg gaccgtcatc ttccacctgg aaccaggcgc
    63841 gattcggctc cgttccgctg ctgactttga tccagccatt gccataacgg gcggcgttga
    63901 ccaccatatt cgccaccgcg cgtttgatcg acagcgggtg cattttcact tcaatgctgc
    63961 cggggtaaag cgcggtttca atttcccgct catagccact ttcggcagca atcacctcac
    64021 cgagtactgc attaagatcc gccatttcca tcggcatctc ctgcccggtg cgcaggtagt
    64081 cgataaactg ctcaatgatg gcgttgcact cttcgatatc tttattgatc gattctgcca
    64141 gatagccatc ctgctcgctc atcatctcag tcgccaggcg aatacgcgtc agcggcgtgc
    64201 gcaagtcgtg acttaccccc gccatcagca gcgtgcggtc atccgccagt tgcttaacac
    64261 cagccgccat atggttaaag gcacgggtaa cggaacgcac ctcggacgcg ccatactcac
    64321 gcagcggcgg cggaataatc cctttaccaa cctgcaaggc tgcgtgttcg agatcgacca
    64381 acggtcggtt ctggatacga ataaacagcc acgccccgcc tatcgccaat agcataatcg
    64441 ccagcgtata gcggaacagc ggagagaaat cgccctgatg aatttcggtc agcggcacgc
    64501 gtacccagat attgggcgac agccaggttt tcagccagac gacaggcgaa cttttgttga
    64561 cctcaacgcg cacttccgtc gggccgccca gttgctgtgc catctgatgg cttaagaatt
    64621 catagtgttg cgcccaacgc agacctgcct cttcggcagc ctcgttggag tagagagaga
    64681 tccccagctc acggtagatc tcccgacgga aagcgggagg cacaaccaac tgcgtgccgt
    64741 cctccagttg cagtttgtcg gtcatcaaca tacgcacttc gtacgcgagg actttattaa
    64801 actgctggag gctcggcaaa atcgcgaagt tcagcaccac cagataagtc gtcaccaggc
    64861 tggcgaacag caaggtgacg atgagcaata acgtacgggc aaatgaactt cgtggcgaga
    64921 agcgcaatcg cctcatgctt tagagccgtc cggtacaaag acgtagccca ggccccaaac
    64981 ggtctgaatg taacgcggat gcgctggatc ttcttctacc atgcggcgca gacgcgaaat
    65041 ctgcacgtcg atggagcgtt ccattgcgga atattcacga ccacgggcaa ggttcatcag
    65101 cttatcgcgg gagagcggct cacgcggatg gctgaccagt gccttcagta ccgcaaactc
    65161 accgctggtg agcggcatcg gctcatcttc gcggaacatt tcgcgcgtac cgaggttaag
    65221 tttgaactta ccgaaagcaa ttaccgcctc ttcctgtgac ggtgcgcctg gcagttcgtt
    65281 cgcctgacga cgcagcaccg cacgaatacg ggccagcagt tcacgcgggt taaacggttt
    65341 tggaatgtag tcgtcagcgc caatctccag gcctacgata cggtccactt cttccccttt
    65401 cgccgtcacc ataatgatcg gcatcgggtt gctctgacta cgaagacgtc ggcaaatcga
    65461 caagccatct tcaccaggta acattaaatc cagtaccata agatggaaag attcacgagt
    65521 cagcaggcga tccatctgtt ctgcattagc gacgcttcga acctggaagc cttgttcggt
    65581 gagataacgt tccagcagcg cacgcaggcg catgtcgtca tcgaccacca gaatcttgta
    65641 gttctcttgc attgtttgta ctcccaaagg ttcgcaacaa tttgtaagcg tgtattctta
    65701 aaaaagctca cgttcgtcac cagctaaatc tggtatgaat ttcagcctaa attgttacaa
    65761 agcatattaa acagcagctt aagtatacaa tttattcggc gaaacattat tgattctgtt
    65821 gatatgatca cgttataccc aatgtgcgca ttatcaaaca gacaaaggga atcaacgaga
    65881 tgaaaacgcc cctggttacc cgggaagggt atgaaaaact caaacaagag cttaattatc
    65941 tctggcgtga agaacgcccg gaggtcacaa aaaaggtgac ctgggccgca agtctgggcg
    66001 accgcagcga aaatgctgac tatcagtata ataaaaagcg tctgcgtgaa atcgaccgtc
    66061 gcgtgcgcta tctcactaaa tgcctggaaa atctcaaaat cgtcgattac tcccctcagc
    66121 aggaaggcaa agtctttttt ggcgcgtggg tggagattga aaacgacgat ggcgtgactc
    66181 accgtttccg tattgtcggc tacgatgaaa tttttggccg taaagattac atctctatcg
    66241 attccccgat ggcccgcgca ttgctgaaaa aagaagtcgg cgatctggcg gtggtgaata
    66301 cccctgccgg ggaagcgagc tggtatgtta atgctatcga gtacgtgaaa ccgtaaggaa
    66361 gagtcttaac ctcctgccga tggctggcat ttttgccagc cagtccgtat aactatcccc
    66421 tgatttttga tccgaaaaga tgaactcaaa ccatgatgaa tgattcgttc tgccgcatta
    66481 ttgcgggtga aattcaggcg cgcccggaac aggttgacgc tgccgttcgc ctgcttgacg
    66541 aagggaatac cgtgccgttt atcgcacgtt atcgtaagga aatcaccggc ggtctggatg
    66601 acacgcagct gcgtaatctg gaaacgcgtc tgagctatct gcgcgagctg gaagagagac
    66661 gtcaggcgat cctcaagtcc atttccgagc aaggcaaact caccgatgat ctggcgaagg
    66721 ccatcaacgc caccctaagc aaaaccgaac tcgaagacct ctacctgccc tacaaaccta
    66781 aacgccgcac ccgcgggcaa atcgccattg aagcagggct tgagccgttg gctgacctgc
    66841 tgtggagcga tccgtcacac acaccagaag tcgccgctgc acaatatgtt gatgccgata
    66901 aaggcgtggc agataccaaa gccgcgctgg atggcgcgcg ctatatcctg atggaacggt
    66961 ttgccgaaga tgccgcgctg ctggcgaaag tgcgtgatta tctgtggaag aacgcgcatt
    67021 tggtttctac ggtggtgagc ggtaaagaag aggaaggggc gaaattccgc gactatttcg
    67081 atcatcacga atcgttgtcc acggtgcctt ctcaccgcgc gctggcgatg ttccgtgggc
    67141 gtaacgaagg cgtactccag ctttcgctga atgccgatcc acagttcgat gagccgccga
    67201 aagagagcta ttgcgagcaa atcatcatgg atcaccttgg cctgcgcctg aacaatgccc
    67261 cggcggatag ctggcgcaaa ggcgtggtga gctggacgtg gcgcatcaag gtgctgatgc
    67321 atctggaaac cgaactgatg ggcaccgtgc gcgaacgtgc ggaagatgaa gcaatcaacg
    67381 tctttgcccg taacctgcac gatctgctga tggcggcccc tgccggactg cgtgcaacga
    67441 tgggcctcga tccgggtctg cgtactgggg taaaagtggc ggtggtcgat gccactggca
    67501 aactggtagc gaccgatacc atttacccgc acaccggaca agccgcaaaa gcagcgatga
    67561 ctgttgctgc gctgtgtgaa aagcataacg ttgaactggt ggcgatcggc aacggtacag
    67621 cttcccgcga aaccgaacgt ttctatctcg acgtgcagaa gcagttcccg aaagtgaccg
    67681 cacagaaagt gatcgtcagc gaagctggcg cgtcggttta ctcggcttcc gagctggcag
    67741 cgcaggagtt tccggatctc gacgtttcgc tgcgtggcgc ggtgtctatc gcccgccgtt
    67801 tgcaggatcc gctggcggag ctggtgaaaa tcgatccgaa atctatcggc gtaggtcagt
    67861 atcagcatga cgtcagccag acgcaactgg cccgcaaact ggacgcagta gtagaagact
    67921 gcgtaaacgc cgttggcgtc gatctcaaca ctgcttctgt tccgctgtta acccgcgtgg
    67981 cgggcctgac gcgcatgatg gcgcaaaaca tcgtggcctg gcgcgatgag aacggtcagt
    68041 tccagaaccg tcagcaactg ctgaaagtga gccgtctggg gccgaaagcc ttcgagcagt
    68101 gcgcgggctt cttgcgcatt aaccatggtg ataacccgct ggacgcgtct accgtccacc
    68161 cggaagccta tccggtggtg gaacgcattc tggcagcaac acagcaggca ctgaaagatc
    68221 tgatgggtaa cagcagcgaa ctgcgtaacc tgaaagcgtc tgactttact gatgaaaaat
    68281 tcggtgtgcc gacagtaact gacatcatca aagagctgga aaaaccgggt cgcgatccgc
    68341 gtccggaatt taaaaccgct cagtttgccg atggcgtcga gacaatgaac gacctgcaac
    68401 cgggtatgat cctcgaaggt gcggtgacca acgtcaccaa ctttggcgcg tttgtcgata
    68461 tcggcgtgca tcaggacggc ctggttcaca tctcttcatt gtcgaacaag tttgtagaag
    68521 atccgcatac cgtggtgaaa gcgggcgaca ttgtgaaggt gaaagtgctg gaagtggatc
    68581 ttcagcgtaa acgtatcgcc ctgaccatgc gtctggacga gcagcctggc gaaaccaacg
    68641 cccgtcgcgg cggcggtaat gaacgcccgc aaaacaaccg cccggcagcc aaaccgcgcg
    68701 gacgtgaagc gcagcctgcc ggtaacagcg cgatgatgga cgcgctggcg gcagcaatgg
    68761 gcaaaaaacg ttaaacgccc tgattgaccc ctaaacctgc gcgataaggt gtccacgccg
    68821 catctggcaa cgaacgcctt gtctgacata aaagtgccgg agaatatctc cggcattttt
    68881 attccacagc caaactcaca atatattccg gcaacattta tcacttcatt aacaactgaa
    68941 accttaatta aacattagcc agtcctggta attcgctatt cgaattatat tttcgctgcg
    69001 atataacctt gagccacatc aacattgagt cagattatta ttcaaaccaa cattcgcaca
    69061 cattttaagt attgctgata gaaaccattc tcattatcat tgtgctgttg attatttaat
    69121 ctctccttcg ttggcaaatc atctggtctc atgtcgctgt caaacgcccc atgaggtagt
    69181 tatccagtta atgagaaata agtaggcacc tatgcaatac actccagata ctgcgtggaa
    69241 aatcactggc ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc tttctcttgg
    69301 catgttacct ggctcctctt ttaatgtggt gcgcgtcgct ccactcggcg accccattca
    69361 tatcgaaacc cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct tattagaagt
    69421 ggaagcggtt tcctgttaat acggtgataa caacaatgaa aaaattaacc ataggcttaa
    69481 ttggtaatcc aaattctggc aagacaacct tatttaacca gctcactggc gcacgtcagc
    69541 gtgtaggtaa ctgggctggc gttaccgtcg aacgtaaaga agggcaattc tccaccaccg
    69601 atcatcaggt cacgctggtg gacctgcccg gcacctattc tctgaccacc atctcatcgc
    69661 agacctcgct cgatgagcaa atcgcctgtc actacatttt gagtggcgac gccgacctgc
    69721 tgattaacgt ggtggatgcg tctaaccttg agcgtaacct gtacctgacg ctacaactgc
    69781 tggaactcgg cattccctgc atcgtggcac tgaacatgct cgacattgcc gagaagcaaa
    69841 atattcgtat tgaaattgat gctctgtcgg cgcgtcttgg ctgcccagtg atcccgctgg
    69901 tttcaacccg tggtcgcggt attgaagcgc tcaagctggc gattgatcgc tataaagcta
    69961 acgagaatgt ggaactggtg cattacgcac agccgctact caacgaagca gattcactgg
    70021 caaaagtgat gccttccgac atcccgctga aacaacgtcg ctggctgggc ctgcaaatgc
    70081 tggaaggcga tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat ctggatgccg
    70141 ccctcgcccg tctgcgtaat gagatggacg atccggcgct gcacattgcc gatgcgcgtt
    70201 accagtgcat tgctgccatc tgtgatgtgg taagcaacac cctgacggca gaacccagcc
    70261 gttttaccac tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg ccgattttcc
    70321 tctttgtgat gtacctgatg ttcctgctgg ctatcaacat cggcggggcg ttacagccgc
    70381 tgtttgacgt cggctccgtg gcgctatttg tgcatggtat tcaatggatt ggctacacgc
    70441 tccacttccc ggactggctg actatcttcc tcgcccaggg cctgggtggc ggcattaaca
    70501 ccgtgctgcc actggtgccg cagattggca tgatgtacct gttcctctcc ttccttgagg
    70561 actccgggta tatggcgcgt gcggcgtttg tgatggaccg tctgatgcag gcgctgggct
    70621 tgccggggaa atcctttgtg ccgctgatcg tcggtttcgg ttgtaacgta ccgtcggtaa
    70681 tgggtgcacg tacgcttgat gcaccgcgtg aacgtctgat gaccatcatg atggcaccgt
    70741 ttatgtcctg cggcgcgcgt ctggctatct tcgcagtatt cgcggctgcc ttcttcgggc
    70801 agaacggtgc gctggcggtc ttctcgctgt atatgctggg tattgtgatg gcggtgctga
    70861 ctggcctgat gctcaagtac accatcatgc gcggtgaagc gacgccgttt gtcatggagt
    70921 tgccggtcta tcatgtacca cacgttaaaa gcctgattat ccagacctgg cagcgtctga
    70981 aaggcttcgt tctgcgtgct ggtaaagtga tcatcatcgt cagcattttc ctgagcgctt
    71041 tcaacagctt ctcgctgagc gggaaaatcg tcgataacat caacgactcg gcgctggcgt
    71101 ccgtcagccg ggtgatcacc ccggtcttca agccaattgg cgtgcatgaa gataactggc
    71161 aggcaacggt tggcctgttt acaggtgcca tggcgaaaga agtggtagtg ggtacgctca
    71221 acaccctcta caccgcagaa aatattcagg acgaagagtt caatccggca gagtttaacc
    71281 tcggtgaaga gctgttcagt gcgatagatg aaacctggca gagcctgaaa gacaccttca
    71341 gccttagcgt actgatgaac cccattgaag ccagcaaagg cgacggcgaa atgggtaccg
    71401 gggcgatggg cgtgatggat cagaaattcg gtagcgcagc agcagcttac agctacctga
    71461 ttttcgtcct gctgtatgta ccatgtatct cggtgatggg agccatcgcc cgtgaatcaa
    71521 gccgtggctg gatgggcttc tccatcctgt gggggctgaa tatcgcttac tcgctggcaa
    71581 cattgttcta tcaggtcgcc agctacagtc agcacccaac ttacagcctg gtgtgcattc
    71641 tggcggttat cctgtttaac atcgtggtta tcggtctgct gcgccgcgcg cgtagccggg
    71701 tggatatcga actgctggca acccgcaagt cggtaagcag ttgctgcgca gccagcacca
    71761 ccggtgattg ccattaatgg cttcacttat tcaggtgcgc gatttgctgg cgttacgggg
    71821 ccgtatggaa gcggcccaga taagccagac attgaacact ccacagccaa tgattaacgc
    71881 catgctgcaa caactggaaa gtatgggcaa agccgtgcgg attcaggaag aacctgacgg
    71941 ctgcctctct ggcagttgta aaagctgccc ggaaggaaaa gcctgtctgc gcgagtggtg
    72001 ggcgctgcgt taaccttact ccatcgcctg ttttggaaag cccggtatgc gtctgcatcc
    72061 gggctttttt gcgtgcggct ttccataaaa atgcaactct tgcagcacgg cgtaagttcc
    72121 tttgaaagca tctcgcaggg atgaaaactc gctaatacac aggtgtgaag tggcgcgtag
    72181 agtcgcggca ttcaaacaac aggtgaagga acgccatgag caaaaagcag agttccaccc
    72241 cacacgatgc gctgttcaaa ctctttttac gccaaccgga cacggctcgt gattttcttg
    72301 cgtttcattt accggcaccc attcacgcgc tttgtgatat gaaaaccctc aagctggagt
    72361 cgagcagctt tattgatgac gatctgcgtg aaagctattc cgatgtgctg tggtcggtga
    72421 aaacggaaca aggaccagga tacatctatt gtctgattga acatcaaagc acctcaaaca
    72481 aactgatcgc atttcgcatg atgcgttacg ctattgccgc aatgcaaaat caccttgatg
    72541 ctggatacaa aacgttgccg atggtggtgc cattgttgtt ttaccacggt attgaaagcc
    72601 cctatcccta ttcgctgtgt tggctggatt gtttcgccga tcccaaactg gcaaggcagc
    72661 tttatgcctc cgcatttccg ctgattgatg tcaccgtcat gcctgatgat gaaatcatgc
    72721 agcaccgacg tatagcgctg ctggagttaa ttcaaaaaca tattcgtcaa cgcgacctga
    72781 tggggctggt agagcaaatg gcctgcttat taagtagtgg atacgctaat gacagacaaa
    72841 tcaaagggct gtttaattac atactgcaaa ccggcgacgc ggtacgtttt aacgatttta
    72901 tcgacggcgt tgccgaacgt tcaccgaaac acaaggagag tttaatgaca attgcggaaa
    72961 gattgcggca ggagggggaa caatccaaag ccctgcatat agccaaaata atgcttgaat
    73021 ccggagtccc tcttacagac atcatgcgct ttaccggggt gtcagaagaa gagttggctg
    73081 cggcgagcca gtaaatttct gtctcgccat ttcaaaagtt gcctacaccc tctgcttcaa
    73141 cgccaccagc aggtgacaaa actcgaccgg atgcgaaata aacggcgcat gggccgcttt
    73201 ggcgaagata tatgatttgc tgcgaggcca gagtttatcc agcatcggca ccactttgcg
    73261 cggcaccaga ccatcgagat agccatacaa tcgcaaaaac ggcatgggca cgttttgcag
    73321 cggctgacgg agatcaaccg ttttcaggat ttccagcccg ccattaagca cgtcaacctc
    73381 cggcatcggt aacgccagaa cggttttctt caacgcccgc gcatcctggc gcgccgtttc
    73441 agtccccatg gtttgtaacg ccaggaaccg ctccactgta cgctgaaaat catcactgag
    73501 ttgctgctga aatcccgcca gcacgtccgg ttttatcccc ggccaatcgt cacgagcact
    73561 aaaacaaggt gacgacgcca cggtgaccag cgcctgaaca cgctcgggat gggttaacgc
    73621 aatctggctt gccaccagcc cgcccagact ccagcctaac caaatggctt tatcaggtgc
    73681 ctgttgcagc acggcttcgg ccatatcagc aagtgacagc gcaccaaatc cctggctgcg
    73741 cccgaagccg ggcagatcaa caaggtgcag cgtaaaatgc gagctaagtt cctcgtcaat
    73801 gcaacaccac acttcggcat tcagtcccca tccgtgcagc agcacaagat gaacattccc
    73861 ctgacctttg gtctgccacc agatgttatt catccgctat tgttctcttt tgacttacaa
    73921 ggatgaacat atgctaacag taccgggatt atgctggcta tgccgaatgc cgctggcgtt
    73981 aggtcattgg gggatttgtt cggtctgctc acgcgccgcc cgtacagata aaacgttatg
    74041 cccccaatgt ggattacccg ccacacactc acatcttccc tgcggtcgct gcctgcaaaa
    74101 accaccgccc tggcaaagac tggtcacggt tgccgactat gcgccgccgt taagtccgct
    74161 tatccaccag cttaaatttt cccggcgcag tgaaatcgcc agcgccctgt cacgtctgtt
    74221 actattggaa gtcttacacg ctcgtcgcac caccgggttg caattgccgg atcgcatcat
    74281 cagcgttccg ttatggcagc ggcgtcactg gcgtcgggga tttaatcaga gcgatttgct
    74341 gtgtcagccg ttatcacgct ggttgcactg ccgatgggat agcgaagccg tcacacgaac
    74401 acgggccact gcgacccagc attttctcag tgcccggcta cgcaagcgca acctgaaaaa
    74461 tgcctttcgt cttgaattgc ccgtgcaagg tcgccatatg gtgattgtgg atgatgtcgt
    74521 taccaccgga agtaccgtcg cagagattgc gcagttgctt ttacgcaatg gtgcggcgac
    74581 tgtccaggtc tggtgccttt gtcgaacctt gtagagcctc gatgatgggc gtattataac
    74641 caactaaaat agtcaactat taggccatta ctatgatccg tatttccgat gctgcacaag
    74701 cgcactttgc caaactgctg gcaaaccagg aagaagggac acaaatccgc gtatttgtga
    74761 ttaaccctgg cacgcctaac gctgaatgtg gtgtttctta ttgtccgccg gatgccgtgg
    74821 aagccaccga cactgccctg aaatttgacc tgctgaccgc gtatgttgat gagttaagcg
    74881 ctccttacct ggaagatgca gagatcgatt ttgttaccga ccagttgggt tctcagctaa
    74941 cactgaaagc gcctaacgcc aaaatgcgta aagtggcaga cgatgcacca ctgatggagc
    75001 gcgtggagta tatgctgcag tcgcagatca acccacagct tgctggtcac ggtggccgcg
    75061 tttcactgat ggaaatcacc gaagacggtt acgccattct gcaatttggc ggcggctgta
    75121 acggttgttc catggtcgat gtgacgctga aagaagggat cgagaagcag ctgctaaacg
    75181 aattcccgga gctgaaaggt gtgcgcgatc tcaccgaaca ccagcgcggc gaacactcct
    75241 actactaagt tatcccctct tttgtagatt gcccgatgcg acgctaaagc gtcatttcgg
    75301 gcctacgttg aacatgtgcc agacgttggc gcagcattgc tttggcaaat ccctcccttc
    75361 gtttctccgc gataatatga ccaacctctc ataatttaaa tttaccccgc tctggtgatt
    75421 ctcaaacgcc agatgttacc cgtatcattc acataggtac caaacatact cctgacatct
    75481 gactacaata attagtttta gtgggtatca gtcgtggtgc cgcaatatct ctgttcccga
    75541 ttgggataat tagagtttgt cgtcagaaaa ttgacgttac ccataacaaa tgaaaggcca
    75601 ggtaaatcat gccattagtc attgttgcta tcggtgtaat attgttgttg ctcctgatga
    75661 tccgcttcaa aatgaacggc ttcatcgctc ttgtcctcgt ggcgcttgct gttggattaa
    75721 tgcaagggat gccgctggat aaagttattg gctccatcaa agccggtgtc ggcgggacgc
    75781 tcggtagcct tgccctgatc atgggttttg gcgcgatgct gggcaaaatg ctggcagact
    75841 gcggtggcgc acaacgtatc gccaccacgc tgattgccaa atttggtaaa aaacacatcc
    75901 agtgggcggt ggtactgacc ggttttaccg ttggttttgc cctgttctat gaagtgggct
    75961 ttgtgctgat gctgccgctg gtgtttacca tcgcggcttc cgcgaatatt ccactgctgt
    76021 atgttggtgt accaatggcg gctgcactgt ctgtgaccca cggcttcctg ccaccgcatc
    76081 cgggcccgac tgcgattgcc accattttca atgccgatat gggtaaaacc ctgctgtacg
    76141 gtactattct ggcaatcccg accgtgattc tcgccggtcc ggtttacgct cgcgtgttga
    76201 aaggtatcga taagccaatc ccggaaggtc tctacagcgc gaaaaccttc agcgaagaag
    76261 agatgccgag ctttggcgtc agcgtctgga cctctctggt gccggtagtg ctgatggcga
    76321 tgcgtgcgat tgccgaaatg atcctgccga aaggtcacgc tttcctgccg gtagcggagt
    76381 tcctcggtga cccggtaatg gctacgctga ttgccgtgct gattgcgatg ttcacctttg
    76441 gtctgaaccg tggtcgttca atggatcaga ttaacgacac gctggtttct tcgatcaaaa
    76501 tcattgcgat gatgctgttg atcatcggtg gtggcggtgc gttcaagcag gtgctggtag
    76561 acagcggcgt ggacaaatac attgcttcca tgatgcacga aaccaacatt tctccgctgc
    76621 tgatggcctg gtcgattgct gccgtactgc gtatcgcgct gggttccgca accgttgcag
    76681 caatcactgc gggtggtatc gcggcaccgc tgattgcaac gacgggtgtt agcccggagc
    76741 tgatggttat tgcggtgggt tccggtagtg tgattttctc tcatgtgaac gatccgggct
    76801 tctggctgtt caaagagtac tttaacctga ctatcggcga gaccatcaaa tcctggtcga
    76861 tgctggaaac cattatctcg gtgtgtggtc tggtaggctg tctgctgctg aatatggtga
    76921 tttgataaca ctgactgccg gatgcggcgt gaccgcctta tccggcctac gattcgggat
    76981 gaattagtag gccggataag acgcgtcaag catcgcatcc ggcatcaacc gcactctact
    77041 tcttcttcgc tgcagctctg cgccgtctgt ccaaatcctt cagcaacttg ttcacgccat
    77101 catcggcaaa catcgactca agcgttgcgg aaagcttgcg tcgccagttt ttatactggt
    77161 aactggtgcc aggaatattc accggttcgg ccatatccag ccagtcttcc ggctgtagtc
    77221 ctaacagagc actgttactg tcggcaatgt agcgctgcaa accacggttc agcgtcggcg
    77281 tcatcgacat caacgatgcc ttatgcccgg cacgttttgg cagacaacca tatttatgca
    77341 gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg atacagaccg cgcagtacca
    77401 cttcatccgg atacagcccc agggttttgc ccagcgttag atccccgctc tcccagtaac
    77461 cgcgtagcgt aggcaggtca tgtgtcgccg caaccgccat cgactgctcc ggatacgctt
    77521 tcggtgcacg gaacgtcttc tcgtggtcgt tttcgaaata gagcactttg taggagtaca
    77581 caccgctgct gcgcagctta ccgacaatct ctaccggtac ggtaccgaga tcttcaccaa
    77641 tcaccataca gcgatgacgt ttactttcga gtgccagaat cgagagcaga tcatccaccg
    77701 gatagtgaac atacgcgccc tgatctgccg tctcgccata cggtatccac cacaaacgca
    77761 gcatcgacat cacatggtca attcgtaatg cgccgcagtt ttgcatattg gcacgcaaca
    77821 gctcgataaa cggttcatag gcacgcgcgg tgatgatatg cgggtccatt ggcggtaatc
    77881 cccagttctg ccccaacggg ccgaggatat ccggcggcgc gccaaccgac gctttcaggc
    77941 aatatagttc acggtcacac caggtttccg ccccaccttc cgctacgcca accgccagat
    78001 cacgatacaa gccaatcggc atttcatagc cctggcttat ctcccagcag gcggcaaact
    78061 ggctgtaagc cagccactgc aaccagagat aaaaatcgac gtcatcacga tgttcttcgc
    78121 agaactgacg cacttctggt gaatccacgt tctgatacat ctctggccat gcaggccagc
    78181 cccagcgcat ttcgtcctct ttcacttgct gggcatgtag cgcatcaaag gctgcctgcc
    78241 agaacaggct gtcgccctgc tctgcaacaa actggcgaaa cgcggtcatc tgctcatcat
    78301 cacgttgcgc gaaacctttc cacgccattc gtaatgctgt catttttagg gcggtaaccg
    78361 tggagtaatc gacccagtcg gcatcgcgcg cctgttgcag cgtctgttgc gtggtcggca
    78421 actgccacca ggcctgagcc tcttcgctaa gatggaaatc ttcaacggcg ttaacgtcga
    78481 tataaatcac attcagccaa cggcgagaag acgggctgta tgggctggca ctctccggat
    78541 ttgccggata gagcgcatga atcgggttca ggccgatgaa cgacccgcca cgttttgcca
    78601 catccaccag catcgctttg agatcgccaa aatccccaat accccagttt ttttccgatc
    78661 gcagcgtata aagctgaacg caggcccccc acagcttttg tttattcagc aacgcctgcg
    78721 gttcgtaaca gcgtttcggg gcgacaatca cccggcaatg cgcgcgctgg tcgtcctggg
    78781 tgagtgtcag cgtgtgataa ccttccggca gcttcgtcgg tagattgaac gctttgcccc
    78841 ccgttacatg gcctttgtac tgtgttcctt cttcggtggt cagcagccag ctatattcgc
    78901 cgctgccctc caccaccatc ggcatttttt tgccgctggt ataaaccatg acattcggga
    78961 ctggcgttac cgccactttc gtggcggtac gttgatgcat cgcgtcaagc aaacgccgtt
    79021 tggtttcggc gctaatcgac tgcggtttac cgtgggcatt gatgtaattg gggctaatcc
    79081 ccgccgccag cgcggcatta tccagacgtt tgctttccat cgagcttcct tagcgttttg
    79141 cctgccagat acgagcctga taatcgcgaa tagagcgatc cgagctaaac ataccgcagc
    79201 gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg gtcgcggtac agcacatcca
    79261 cctgcttttg tgcctcaaca taggctgcga aatccgccat caccagatac ggatcgccgc
    79321 cttgtttgcc gatactgtgc agcatctggt cgaaggcatg cttatcgccg tcgctgtatt
    79381 taccgctttc cagctctttc aatactgcgt ccagcacctt atctttcttc cgccatttca
    79441 ccgggtcgta gcctttggcc agaattgcct tcacttgttc cacggtatga ccaaaaataa
    79501 agatattttc ttcaccgact ttctcggcga tttcaacgtt cgcaccatcc agcgtaccga
    79561 cagtaagcgc accattgagc gccagtttca tattgccggt accggaagct tctttacctg
    79621 cagtcgaaat ttgttcggag atatccgccg ccgggatcag tttttccgcc gccgaaacgc
    79681 aataatccgg caggaacacc accttcaact tatcgccaac cagtggatcg ttgttgatca
    79741 cgtcagccac tttgttgatc gcaaagataa tattcttcgc caggtagtag cccggtgccg
    79801 ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc ctgcgggttt tcacgaattt
    79861 ctttgtacaa cgccagaata tgcagcagat tcaggtgctg gcgtttgtac tcatgcagac
    79921 gtttgatctg aatatcgaaa atcgcctgtg gattgatctc aataccggta cgaactttca
    79981 caaactccgc cagacggact ttattcgcct gcttgatctc gcgatattgc tgacggaatt
    80041 tcgcatcatc agcgaatttt tccagattga tcagctgatc gagatcgtta gcccactctt
    80101 tttgcagtga tttatccaac agagccgcca gtgcagggtt gcactgtttg atccagcgac
    80161 gtggggtaat accgttggtg acgttatgga atttgttcgg ccatagctgg tgatattccg
    80221 ggaacagatc tttcaccacc agatccgagt gcagcgccgc aacaccgttc accgcgaaac
    80281 cgccaaccac acacaggttc gccatatgca cttgtttatc gtgcaccacc gccagtttgg
    80341 cccacacttt ttcatcgccc ggccaggttt tctccaccag cgttttaaag cgagtattaa
    80401 tttcgttaat aatctgcata tggcgcggca gtaagccttt caccagtttc acatcccagc
    80461 gttccagcgc ttctggcatc agggtatggt tggtgtaagc gaaagttttg ctggtaatag
    80521 cccaggcgtc atcccagctc atctggtgct catcgatcag cacgcgcagc agttctggaa
    80581 tcgcgatagt tgggtgggta tcgttcagct gaataacttc gtaatccgcc agttcgtgca
    80641 gtttacgccc cgccagatga tggcgacgca aaatatccgc taccgaacag gcacactgga
    80701 agtactgctg catcaagcgc agctttttac cggcagtatg gttgtcgttt ggatagagaa
    80761 ctttggtcag tttttccgca ttgatgccct gctgttcggc acgcaggaaa tcaccgtcgt
    80821 taaatttagt cagatcaaac ggatgcgcgt gcgtcgcctg ccacagacgc agcggctgcg
    80881 ccacgccatt acgatagccg acaacgggga gatcccacgc ttgaccggta atggtaaact
    80941 ccggctccca gcgtccgtct ttcgtcactt taccgccaat ccctacctgc acatccagtg
    81001 cttcgttgtg gcggaaccac gggtagttac tgcgatgcca gtcatccggc gcttcaacct
    81061 gtttgccatc gacaaaagac tggcggaaca aaccatattg atagttcaga ccgtaacccg
    81121 tcgcagactg accgacagtt gccattgagt cgaggaagca cgccgccaga cgtcccagac
    81181 caccgttacc cagcgccggg tcgatctctt cttccagcag gtccgtcaga ttgatgtcat
    81241 aagccttcaa cgaatcctgt acatcctgat accagccgag attcaacagg ttgttgcccg
    81301 tcaggcgacc aatcaaaaac tccattgaga tgtagttaac atgtcgctga ttcgccaccg
    81361 gcttggcgaa tggctgagca cgcagcattt cggccagtgc ttcactcact gctagccacc
    81421 actggcgagg agtcatttca gccgcagaat ttaagccata acgctgccac tgacgtgaaa
    81481 gcgcttcctg aaattgctta tcgttaaaaa taggttgtga cataggagtt ccacttttct
    81541 tagattttca acacaacgtt atcgctagtt tgccaggctc gatgttgacc ttcctcatcc
    81601 tgcgggggat taggcaggga ggagttgcgg ggatgagcaa ggaaatgtga tctcaaccac
    81661 ttaaagctag tgcaaaccac aggattagca gcaaatcaat gcaatacagc gcagaaaatc
    81721 tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa aagcggaagg taactctata
    81781 aattaagtaa aggagtgaaa cagtttcata agtaaaatat ccagtgtgct ccatctcatt
    81841 cttaatagat ttattaagat catcttttta gatggcactt tcatcaggaa tgaagaagaa
    81901 acccttgctt aaatgaatct gatgaacata agggaaacca gtattcacgc tggatcagcg
    81961 tcgttttagg tgagttgtta ataaagattt ggaattgtga cacagtgcaa attcagacac
    82021 ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc cgaccttata accattaatt
    82081 acgaagcgca aaaaaaataa tatttcctca ttttccacag tgaagtgatt aactatgctg
    82141 attccgtcaa aactaagtcg tccggttcga ctcgaccata ccgtggttcg tgagcgcctg
    82201 ctggctaaac tttccggcgc gaacaacttc cggctggcgc tgatcacgag tcctgcgggc
    82261 tacggaaaga ccaccctcat ttcccagtgg gcggcaggca aaaacgatat cggctggtac
    82321 tcgctggatg aaggtgataa ccagcaagag cgtttcgcca gctatctcat tgccgccgtg
    82381 cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga tggcgcaaaa acggcaatat
    82441 gccagcctga cgtcactctt cgcccagctt ttcattgagc tggcggaatg gcatagccaa
    82501 ctttatctgg tcatcgatga ctatcatctg atcactaatc cagtgatcca cgagtcaatg
    82561 cgcttcttta ttcgccatca accagaaaat ctcaccctgg tggtgttgtc acgcaatctt
    82621 ccgcaactgg gcattgccaa tctgcgtgtt cgtgatcaac tgctggaaat tggcagtcag
    82681 caacttgcat ttacccatca ggaagcgaag cagttttttg attgccgtct gtcatcgccg
    82741 attgaagccg cagaaagcag tcggatttgc gatgacgttt ccggttgggc gacggcacta
    82801 cagctaatcg ccctctccgc ccggcagaat acccactcag cccataagtc ggcacgccgc
    82861 ctggcgggaa tcaatgccag ccatctttcg gattatctgg tcgatgaggt tttggataac
    82921 gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca ttttgcgctc aatgaacgat
    82981 gcactcatca cccgtgtgac cggcgaagaa aacgggcaaa tgcgcctcga agagattgag
    83041 cgtcaggggc tgtttttaca gcggatggat gataccggcg agtggttctg ctatcacccg
    83101 ctgtttggca acttcctgcg ccagcgctgc cagtgggaac tggcggcgga gctgccggaa
    83161 atccaccgtg ccgccgcaga aagctggatg gcccagggat ttcccagcga agcgattcat
    83221 catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata ttctgcttaa tcacgcctgg
    83281 agtttgttca accatagcga actgtcgctg ctggaagagt cgcttaaggc cctgccgtgg
    83341 gacagtttgc tggaaaatcc gcagttagtg ttattgcagg cgtggctaat gcaaagccaa
    83401 catcgctatg gcgaagttaa caccctgcta gcccgtgctg aacatgaaat caaggacatc
    83461 agagaaggca ccatgcacgc agaatttaac gctctgcgcg cccaggtggc gattaacgat
    83521 ggtaatccgg atgaagcgga acggctggca aaactggcac tggaagagct gccgccgggc
    83581 tggttctata gccgcattgt ggcaacctcg gtgctgggtg aagtgctgca ctgcaaaggc
    83641 gaattgaccc gctcactggc gctaatgcag caaaccgaac agatggcacg ccagcacgac
    83701 gtctggcact acgccttatg gagtttaatc cagcaaagtg aaattctgtt tgcccaaggg
    83761 ttcctgcaaa ccgcgtggga aacacaggaa aaagcgttcc agctgatcaa cgagcagcat
    83821 ctggaacagc tgccaatgca tgagtttctg gtgcgcatcc gagcacaact gttatgggcc
    83881 tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg ggattgaagt cttgtcgtct
    83941 tatcagccac agcaacagct tcagtgcctg gcaatgttga ttcaatgctc gctggcccgt
    84001 ggtgatttag ataacgcccg tagccagctg aaccgtctgg aaaacctgct ggggaatggc
    84061 gaatatcaca gcgactggat ctctaacgcc aacaaagtcc gggtgattta ctggcaaatg
    84121 accggcgata aagccgccgc tgccaactgg ttgcgtcata cggctaaacc ggagtttgcg
    84181 aacaaccact tcctgcaagg tcaatggcgc aacattgccc gcgcacaaat cttgctgggc
    84241 gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg aaaatgcccg cagtctgcgg
    84301 ttgatgagcg atctcaaccg taacctgttg ctgcttaatc aactgtactg gcaggccgga
    84361 cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa aactggcgaa tcgcaccgga
    84421 tttatcagcc attttgtcat cgaaggcgaa gcgatggcgc aacaactgcg ccagctgatt
    84481 caactcaata cgctgccgga actggaacag catcgcgcac agcgtattct gcgagaaatc
    84541 aatcaacatc atcggcataa attcgcccat ttcgatgaga atttcgttga acgtctgctg
    84601 aatcatccgg aagtgccaga actgatccgc accagcccgt tgacccaacg tgaatggcag
    84661 gtcctgggac tgatttactc cggttacagc aatgagcaaa tagcgggtga actggaagtg
    84721 gcggcaacca ccatcaagac gcatatccgc aatctgtacc aaaaactcgg cgtcgcccac
    84781 cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga tgatgggtta tggggtgtga
    84841 gtttagccgg ataatgcatt ggatccggtt tacatcttta catcattcaa tactcacccg
    84901 cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc aagaaacgct ccaccaccgc
    84961 gatattggtc agcagatggc atgaaggatg ggcgaccgta aattctcccg cgcccgccag
    85021 cgccatcggc agtaccagct ggtcggcgag atattccccc actgccgccg ggcttgccag
    85081 gtagcgtttc acctctttca ctaactgcgc ggctaccacc tcggcactga cgcgcttttc
    85141 accgacgaca aaaaagcgtt cggtgatatt ttcactttcg acttcaagcg agacggtatt
    85201 acccggcccc tggtcacgcg gcaggttatg aatattctgc tcatgcaggg aaaaactacc
    85261 cgccagcgta gcgatttcac gctcagcgac atggcgcggc acgccagcta ataacacctc
    85321 tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc aaggtgttaa acgatgccac
    85381 cggcgagact tccgttgcca ccacgccgcc tccggcagga taaaaaccgt gacgtaacag
    85441 cgtggtttgc tgatgaattc ctattttcgc cagcagcggc tccagcaccc ggcggataaa
    85501 atccgcaggc ggggccgacg ggttatcggt gcctccgctc acttcaacac gcgaaggtcc
    85561 atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc agcgtacagc ttccggcact
    85621 accgatagca aagcggtact cgccgccgcg cacggtgcca ggccggaaga gcagacgctg
    85681 cgaccccagc tccgcgcctt ccaccgttgc cctacaaatt tccgcagccg cttttaccgc
    85741 ggtcagatgc tggcgcaaca gccccgattt cgcccgtccg gctcgaatgc cagtgatggt
    85801 aaatggctgg ccggttatca tcgacaggct cagcgccgag cgcaggatct gtccgccgcc
    85861 ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc atttatcctt ttacgcacac
    85921 cacctgacgc agggtgtaga taacttccac cagatcgctt tgtgccgcca tcaccgcatc
    85981 aatatcttta tacgccatcg ggatttcgtc gatcacttcg gcatctttac ggcattccac
    86041 atgcgcggtg gcacgaattt gatcttccac gctgaacagt tttttcgctt tagtacggct
    86101 cattacccgc ccggcaccgt ggctgcacga acagaacgac tcttcatttc ccagcccacg
    86161 gacgataaag ctttttgctc ccatcgaacc gggaataatt ccatattgac cagcacgcgc
    86221 agacaccgcg cctttacgcg tcacgtagat ctcttcacca aagtgctgtt ctttttgcac
    86281 atagttgtgg tgacagttga tctcttccat cgccagcgtt tgtggctgtc tgaccgtttt
    86341 ctgcgtaatg ctctgcaacg ccgttaccac attttccatc atcgcatcgc ggttaaggct
    86401 ggcgaaaagc tgcgcccagg ccacggctct taggtaatca tcaaagtatt ccgtaccttc
    86461 cataaagtac gccagatcac gcgacggcaa cgtctcaagt tggtcctgca tctctttttg
    86521 cgccagatcg ataaagtaag tcccgatggc gttaccaatt ccgcgtgaac cggagtgcag
    86581 cataatccac acctgctccg actcatcaag gcagatttca ataaagtggt tacccgttcc
    86641 cagcgttctc aggtgtttat agttgttagt gttaaggaaa cgcggatatt tttgcgttaa
    86701 ccactgataa ccggcttcaa gctcagccca tttcgcatcg acgttaacag gcgggttttc
    86761 ccatgcgcct ttatcacgtt tacaacgccc ggtagtacgc ccgtgcggca ccgcagtttc
    86821 aatcgcctga cgcagctctg ccaggttttc aggcaggtct gccgccgtta acgcggtacg
    86881 cagcgcgttc attccacagc caatatccac gcccaccgcc gccggaataa tcgccccttt
    86941 ggtcgggatc acgctaccaa tggtggaacc tttacccagg tgtacatcag gcattaccgc
    87001 aatatgtttg aaaataaacg gcatcttcgc cgtattaata agttgctgac gcgcatcggc
    87061 ttctaccggc acgcctttgg tccacatttt taccggggca ttttcagtgg tcagtaattc
    87121 gtaattcatt ttgtttttct ctt
//
LOCUS       DAZKHC020000007        79062 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZKHC020000007 DAZKHC020000000
VERSION     DAZKHC020000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 79062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.007"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            39..623
                     /gene="yraH"
                     /locus_tag="ACHOSM_RS02965"
                     /old_locus_tag="pgaptmp_000593"
     CDS             39..623
                     /gene="yraH"
                     /locus_tag="ACHOSM_RS02965"
                     /old_locus_tag="pgaptmp_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4557177.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            703..1398
                     /gene="yraI"
                     /locus_tag="ACHOSM_RS02970"
                     /old_locus_tag="pgaptmp_000594"
     CDS             703..1398
                     /gene="yraI"
                     /locus_tag="ACHOSM_RS02970"
                     /old_locus_tag="pgaptmp_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4557178.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1428..3944
                     /gene="yraJ"
                     /locus_tag="ACHOSM_RS02975"
                     /old_locus_tag="pgaptmp_000595"
     CDS             1428..3944
                     /gene="yraJ"
                     /locus_tag="ACHOSM_RS02975"
                     /old_locus_tag="pgaptmp_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4557179.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGVFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFNEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLIIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3955..5046
                     /gene="yraK"
                     /locus_tag="ACHOSM_RS02980"
                     /old_locus_tag="pgaptmp_000596"
     CDS             3955..5046
                     /gene="yraK"
                     /locus_tag="ACHOSM_RS02980"
                     /old_locus_tag="pgaptmp_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4557180.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDEMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5089..5949)
                     /gene="rsmI"
                     /locus_tag="ACHOSM_RS02985"
                     /old_locus_tag="pgaptmp_000597"
     CDS             complement(5089..5949)
                     /gene="rsmI"
                     /locus_tag="ACHOSM_RS02985"
                     /old_locus_tag="pgaptmp_000597"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGU4557181.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVELIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            6013..8049
                     /gene="lpoA"
                     /locus_tag="ACHOSM_RS02990"
                     /old_locus_tag="pgaptmp_000598"
     CDS             6013..8049
                     /gene="lpoA"
                     /locus_tag="ACHOSM_RS02990"
                     /old_locus_tag="pgaptmp_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HGU4557182.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITLRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8007..8402
                     /gene="yraN"
                     /locus_tag="ACHOSM_RS02995"
                     /old_locus_tag="pgaptmp_000599"
     CDS             8007..8402
                     /gene="yraN"
                     /locus_tag="ACHOSM_RS02995"
                     /old_locus_tag="pgaptmp_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HGU4557183.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDVWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8422..9012
                     /gene="diaA"
                     /locus_tag="ACHOSM_RS03000"
                     /old_locus_tag="pgaptmp_000600"
     CDS             8422..9012
                     /gene="diaA"
                     /locus_tag="ACHOSM_RS03000"
                     /old_locus_tag="pgaptmp_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HGU4557184.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            9022..9597
                     /gene="dolP"
                     /locus_tag="ACHOSM_RS03005"
                     /old_locus_tag="pgaptmp_000601"
     CDS             9022..9597
                     /gene="dolP"
                     /locus_tag="ACHOSM_RS03005"
                     /old_locus_tag="pgaptmp_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HGU4557185.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9710..10750)
                     /gene="yraQ"
                     /locus_tag="ACHOSM_RS03010"
                     /old_locus_tag="pgaptmp_000602"
     CDS             complement(9710..10750)
                     /gene="yraQ"
                     /locus_tag="ACHOSM_RS03010"
                     /old_locus_tag="pgaptmp_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGU4557186.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLVMLRNAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10823..11458)
                     /gene="yraR"
                     /locus_tag="ACHOSM_RS03015"
                     /old_locus_tag="pgaptmp_000603"
     CDS             complement(10823..11458)
                     /gene="yraR"
                     /locus_tag="ACHOSM_RS03015"
                     /old_locus_tag="pgaptmp_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGU4557187.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11586..12104
                     /gene="yhbO"
                     /locus_tag="ACHOSM_RS03020"
                     /old_locus_tag="pgaptmp_000604"
     CDS             11586..12104
                     /gene="yhbO"
                     /locus_tag="ACHOSM_RS03020"
                     /old_locus_tag="pgaptmp_000604"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HGU4557188.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDDRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12084..12527)
                     /gene="yhbP"
                     /locus_tag="ACHOSM_RS03025"
                     /old_locus_tag="pgaptmp_000605"
     CDS             complement(12084..12527)
                     /gene="yhbP"
                     /locus_tag="ACHOSM_RS03025"
                     /old_locus_tag="pgaptmp_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HGU4557189.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12578..12880
                     /gene="yhbQ"
                     /locus_tag="ACHOSM_RS03030"
                     /old_locus_tag="pgaptmp_000606"
     CDS             12578..12880
                     /gene="yhbQ"
                     /locus_tag="ACHOSM_RS03030"
                     /old_locus_tag="pgaptmp_000606"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HGU4557190.1"
                     /translation="MTPWFLYLIRTTDNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKLQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12867..13370)
                     /gene="yhbS"
                     /locus_tag="ACHOSM_RS03035"
                     /old_locus_tag="pgaptmp_000607"
     CDS             complement(12867..13370)
                     /gene="yhbS"
                     /locus_tag="ACHOSM_RS03035"
                     /old_locus_tag="pgaptmp_000607"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4557191.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREEGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13364..13888)
                     /gene="ubiT"
                     /locus_tag="ACHOSM_RS03040"
                     /old_locus_tag="pgaptmp_000608"
     CDS             complement(13364..13888)
                     /gene="ubiT"
                     /locus_tag="ACHOSM_RS03040"
                     /old_locus_tag="pgaptmp_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HGU4557192.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRRLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14097..15092
                     /gene="ubiU"
                     /locus_tag="ACHOSM_RS03045"
                     /old_locus_tag="pgaptmp_000609"
     CDS             14097..15092
                     /gene="ubiU"
                     /locus_tag="ACHOSM_RS03045"
                     /old_locus_tag="pgaptmp_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGU4557193.1"
                     /translation="MELLCPAGNLPALKAAIENSADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15101..15979
                     /gene="ubiV"
                     /locus_tag="ACHOSM_RS03050"
                     /old_locus_tag="pgaptmp_000610"
     CDS             15101..15979
                     /gene="ubiV"
                     /locus_tag="ACHOSM_RS03050"
                     /old_locus_tag="pgaptmp_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGU4557194.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNEMTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16185..17192
                     /gene="yhbW"
                     /locus_tag="ACHOSM_RS03055"
                     /old_locus_tag="pgaptmp_000611"
     CDS             16185..17192
                     /gene="yhbW"
                     /locus_tag="ACHOSM_RS03055"
                     /old_locus_tag="pgaptmp_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HGU4557195.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17310..18554)
                     /gene="mtr"
                     /locus_tag="ACHOSM_RS03060"
                     /old_locus_tag="pgaptmp_000612"
     CDS             complement(17310..18554)
                     /gene="mtr"
                     /locus_tag="ACHOSM_RS03060"
                     /old_locus_tag="pgaptmp_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HGU4557196.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFCVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18708..20597)
                     /gene="deaD"
                     /locus_tag="ACHOSM_RS03065"
                     /old_locus_tag="pgaptmp_000613"
     CDS             complement(18708..20597)
                     /gene="deaD"
                     /locus_tag="ACHOSM_RS03065"
                     /old_locus_tag="pgaptmp_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HGU4557197.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20590..20670)
                     /gene="yrbN"
                     /locus_tag="ACHOSM_RS03070"
                     /old_locus_tag="pgaptmp_000614"
     CDS             complement(20590..20670)
                     /gene="yrbN"
                     /locus_tag="ACHOSM_RS03070"
                     /old_locus_tag="pgaptmp_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HGU4557198.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20777..21661)
                     /gene="nlpI"
                     /locus_tag="ACHOSM_RS03075"
                     /old_locus_tag="pgaptmp_000615"
     CDS             complement(20777..21661)
                     /gene="nlpI"
                     /locus_tag="ACHOSM_RS03075"
                     /old_locus_tag="pgaptmp_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HGU4557199.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTFNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21770..23905)
                     /gene="pnp"
                     /locus_tag="ACHOSM_RS03080"
                     /old_locus_tag="pgaptmp_000616"
     CDS             complement(21770..23905)
                     /gene="pnp"
                     /locus_tag="ACHOSM_RS03080"
                     /old_locus_tag="pgaptmp_000616"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGU4557200.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGEHTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24152..24421)
                     /gene="rpsO"
                     /locus_tag="ACHOSM_RS03085"
                     /old_locus_tag="pgaptmp_000617"
     CDS             complement(24152..24421)
                     /gene="rpsO"
                     /locus_tag="ACHOSM_RS03085"
                     /old_locus_tag="pgaptmp_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGU4557201.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24570..25514)
                     /gene="truB"
                     /locus_tag="ACHOSM_RS03090"
                     /old_locus_tag="pgaptmp_000618"
     CDS             complement(24570..25514)
                     /gene="truB"
                     /locus_tag="ACHOSM_RS03090"
                     /old_locus_tag="pgaptmp_000618"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGU4557202.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25514..25915)
                     /gene="rbfA"
                     /locus_tag="ACHOSM_RS03095"
                     /old_locus_tag="pgaptmp_000619"
     CDS             complement(25514..25915)
                     /gene="rbfA"
                     /locus_tag="ACHOSM_RS03095"
                     /old_locus_tag="pgaptmp_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGU4557203.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26079..28751)
                     /gene="infB"
                     /locus_tag="ACHOSM_RS03100"
                     /old_locus_tag="pgaptmp_000620"
     CDS             complement(26079..28751)
                     /gene="infB"
                     /locus_tag="ACHOSM_RS03100"
                     /old_locus_tag="pgaptmp_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGU4557204.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28776..30263)
                     /gene="nusA"
                     /locus_tag="ACHOSM_RS03105"
                     /old_locus_tag="pgaptmp_000621"
     CDS             complement(28776..30263)
                     /gene="nusA"
                     /locus_tag="ACHOSM_RS03105"
                     /old_locus_tag="pgaptmp_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGU4557205.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30291..30743)
                     /gene="rimP"
                     /locus_tag="ACHOSM_RS03110"
                     /old_locus_tag="pgaptmp_000622"
     CDS             complement(30291..30743)
                     /gene="rimP"
                     /locus_tag="ACHOSM_RS03110"
                     /old_locus_tag="pgaptmp_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGU4557206.1"
                     /translation="MSTLEQKLTEMITAPVEALGFGLVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30950..31026)
                     /locus_tag="ACHOSM_RS03115"
                     /old_locus_tag="pgaptmp_000623"
     tRNA            complement(30950..31026)
                     /locus_tag="ACHOSM_RS03115"
                     /old_locus_tag="pgaptmp_000623"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30990..30992),aa:Met,seq:cat)
     gene            31374..32717
                     /gene="argG"
                     /locus_tag="ACHOSM_RS03120"
                     /old_locus_tag="pgaptmp_000624"
     CDS             31374..32717
                     /gene="argG"
                     /locus_tag="ACHOSM_RS03120"
                     /old_locus_tag="pgaptmp_000624"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HGU4557207.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKVK"
     gene            32901..33854
                     /locus_tag="ACHOSM_RS03125"
                     /old_locus_tag="pgaptmp_000625"
     CDS             32901..33854
                     /locus_tag="ACHOSM_RS03125"
                     /old_locus_tag="pgaptmp_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4557208.1"
                     /translation="MNGEYFVRLALHTLKCTQKDLASHLGVSSTQISKWKKGEHMSAD
                     MEKKFRNITQIGDYSPQLVEWTGSVANAEKWDRLIHFLARRAMDDAETGYITQLLTDE
                     DGFLVEETIDVLKRIGFPTPLSFPEGLNIDDDNADEEEAFWEILESNAHCSVINDIYH
                     ALNDVYGFYIAYVDELIQDDDLDVYSSEAINIQSSLISLAACKIEIDTPVASNFKEFR
                     YRVKKDYENWLNQLKMMAFRAGIPLRAELLEMVYNTADQLSVAAEAERFDFNKSRIHP
                     DIYMNEILTGMRIIHQVLPVIMQKLEITDFKLDETDLCLGK"
     gene            complement(33929..34015)
                     /locus_tag="ACHOSM_RS03130"
                     /old_locus_tag="pgaptmp_000626"
     tRNA            complement(33929..34015)
                     /locus_tag="ACHOSM_RS03130"
                     /old_locus_tag="pgaptmp_000626"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33979..33981),aa:Leu,seq:gag)
     gene            complement(34030..34362)
                     /gene="secG"
                     /locus_tag="ACHOSM_RS03135"
                     /old_locus_tag="pgaptmp_000627"
     CDS             complement(34030..34362)
                     /gene="secG"
                     /locus_tag="ACHOSM_RS03135"
                     /old_locus_tag="pgaptmp_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGU4557209.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(34590..35927)
                     /gene="glmM"
                     /locus_tag="ACHOSM_RS03140"
                     /old_locus_tag="pgaptmp_000628"
     CDS             complement(34590..35927)
                     /gene="glmM"
                     /locus_tag="ACHOSM_RS03140"
                     /old_locus_tag="pgaptmp_000628"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGU4557210.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(35920..36768)
                     /gene="folP"
                     /locus_tag="ACHOSM_RS03145"
                     /old_locus_tag="pgaptmp_000629"
     CDS             complement(35920..36768)
                     /gene="folP"
                     /locus_tag="ACHOSM_RS03145"
                     /old_locus_tag="pgaptmp_000629"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGU4557211.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGMHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(36858..38792)
                     /gene="ftsH"
                     /locus_tag="ACHOSM_RS03150"
                     /old_locus_tag="pgaptmp_000630"
     CDS             complement(36858..38792)
                     /gene="ftsH"
                     /locus_tag="ACHOSM_RS03150"
                     /old_locus_tag="pgaptmp_000630"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGU4557212.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVSGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(38892..39521)
                     /gene="rlmE"
                     /locus_tag="ACHOSM_RS03155"
                     /old_locus_tag="pgaptmp_000631"
     CDS             complement(38892..39521)
                     /gene="rlmE"
                     /locus_tag="ACHOSM_RS03155"
                     /old_locus_tag="pgaptmp_000631"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HGU4557213.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            39647..39940
                     /gene="yhbY"
                     /locus_tag="ACHOSM_RS03160"
                     /old_locus_tag="pgaptmp_000632"
     CDS             39647..39940
                     /gene="yhbY"
                     /locus_tag="ACHOSM_RS03160"
                     /old_locus_tag="pgaptmp_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGU4557214.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40096..40572)
                     /gene="greA"
                     /locus_tag="ACHOSM_RS03165"
                     /old_locus_tag="pgaptmp_000633"
     CDS             complement(40096..40572)
                     /gene="greA"
                     /locus_tag="ACHOSM_RS03165"
                     /old_locus_tag="pgaptmp_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGU4557215.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            40820..42253
                     /gene="dacB"
                     /locus_tag="ACHOSM_RS03170"
                     /old_locus_tag="pgaptmp_000634"
     CDS             40820..42253
                     /gene="dacB"
                     /locus_tag="ACHOSM_RS03170"
                     /old_locus_tag="pgaptmp_000634"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU4557216.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQVGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(42293..43465)
                     /gene="cgtA"
                     /locus_tag="ACHOSM_RS03175"
                     /old_locus_tag="pgaptmp_000635"
     CDS             complement(42293..43465)
                     /gene="cgtA"
                     /locus_tag="ACHOSM_RS03175"
                     /old_locus_tag="pgaptmp_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HGU4557217.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLLAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEVEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(43481..44446)
                     /gene="yhbE"
                     /locus_tag="ACHOSM_RS03180"
                     /old_locus_tag="pgaptmp_000636"
     CDS             complement(43481..44446)
                     /gene="yhbE"
                     /locus_tag="ACHOSM_RS03180"
                     /old_locus_tag="pgaptmp_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4557218.1"
                     /translation="MKQQAGIGILLALTTVICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(44573..44830)
                     /gene="rpmA"
                     /locus_tag="ACHOSM_RS03185"
                     /old_locus_tag="pgaptmp_000637"
     CDS             complement(44573..44830)
                     /gene="rpmA"
                     /locus_tag="ACHOSM_RS03185"
                     /old_locus_tag="pgaptmp_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGU4557219.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(44851..45162)
                     /gene="rplU"
                     /locus_tag="ACHOSM_RS03190"
                     /old_locus_tag="pgaptmp_000638"
     CDS             complement(44851..45162)
                     /gene="rplU"
                     /locus_tag="ACHOSM_RS03190"
                     /old_locus_tag="pgaptmp_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGU4557220.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            45421..46392
                     /gene="ispB"
                     /locus_tag="ACHOSM_RS03195"
                     /old_locus_tag="pgaptmp_000639"
     CDS             45421..46392
                     /gene="ispB"
                     /locus_tag="ACHOSM_RS03195"
                     /old_locus_tag="pgaptmp_000639"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HGU4557221.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            46620..46898
                     /gene="sfsB"
                     /locus_tag="ACHOSM_RS03200"
                     /old_locus_tag="pgaptmp_000640"
     CDS             46620..46898
                     /gene="sfsB"
                     /locus_tag="ACHOSM_RS03200"
                     /old_locus_tag="pgaptmp_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HGU4557222.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PGPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(46946..48205)
                     /gene="murA"
                     /locus_tag="ACHOSM_RS03205"
                     /old_locus_tag="pgaptmp_000641"
     CDS             complement(46946..48205)
                     /gene="murA"
                     /locus_tag="ACHOSM_RS03205"
                     /old_locus_tag="pgaptmp_000641"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGU4557223.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(48260..48514)
                     /gene="ibaG"
                     /locus_tag="ACHOSM_RS03210"
                     /old_locus_tag="pgaptmp_000642"
     CDS             complement(48260..48514)
                     /gene="ibaG"
                     /locus_tag="ACHOSM_RS03210"
                     /old_locus_tag="pgaptmp_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HGU4557224.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(48674..48967)
                     /gene="mlaB"
                     /locus_tag="ACHOSM_RS03215"
                     /old_locus_tag="pgaptmp_000643"
     CDS             complement(48674..48967)
                     /gene="mlaB"
                     /locus_tag="ACHOSM_RS03215"
                     /old_locus_tag="pgaptmp_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HGU4557225.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGVTCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPH"
     gene            complement(48967..49602)
                     /gene="mlaC"
                     /locus_tag="ACHOSM_RS03220"
                     /old_locus_tag="pgaptmp_000644"
     CDS             complement(48967..49602)
                     /gene="mlaC"
                     /locus_tag="ACHOSM_RS03220"
                     /old_locus_tag="pgaptmp_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HGU4557226.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(49621..50172)
                     /gene="mlaD"
                     /locus_tag="ACHOSM_RS03225"
                     /old_locus_tag="pgaptmp_000645"
     CDS             complement(49621..50172)
                     /gene="mlaD"
                     /locus_tag="ACHOSM_RS03225"
                     /old_locus_tag="pgaptmp_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HGU4557227.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(50177..50959)
                     /gene="mlaE"
                     /locus_tag="ACHOSM_RS03230"
                     /old_locus_tag="pgaptmp_000646"
     CDS             complement(50177..50959)
                     /gene="mlaE"
                     /locus_tag="ACHOSM_RS03230"
                     /old_locus_tag="pgaptmp_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HGU4557228.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(50967..51776)
                     /gene="mlaF"
                     /locus_tag="ACHOSM_RS03235"
                     /old_locus_tag="pgaptmp_000647"
     CDS             complement(50967..51776)
                     /gene="mlaF"
                     /locus_tag="ACHOSM_RS03235"
                     /old_locus_tag="pgaptmp_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HGU4557229.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            51986..52963
                     /gene="yrbG"
                     /locus_tag="ACHOSM_RS03240"
                     /old_locus_tag="pgaptmp_000648"
     CDS             51986..52963
                     /gene="yrbG"
                     /locus_tag="ACHOSM_RS03240"
                     /old_locus_tag="pgaptmp_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HGU4557230.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            52977..53963
                     /gene="kdsD"
                     /locus_tag="ACHOSM_RS03245"
                     /old_locus_tag="pgaptmp_000649"
     CDS             52977..53963
                     /gene="kdsD"
                     /locus_tag="ACHOSM_RS03245"
                     /old_locus_tag="pgaptmp_000649"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HGU4557231.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            53984..54550
                     /gene="kdsC"
                     /locus_tag="ACHOSM_RS03250"
                     /old_locus_tag="pgaptmp_000650"
     CDS             53984..54550
                     /gene="kdsC"
                     /locus_tag="ACHOSM_RS03250"
                     /old_locus_tag="pgaptmp_000650"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HGU4557232.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            54547..55122
                     /gene="lptC"
                     /locus_tag="ACHOSM_RS03255"
                     /old_locus_tag="pgaptmp_000651"
     CDS             54547..55122
                     /gene="lptC"
                     /locus_tag="ACHOSM_RS03255"
                     /old_locus_tag="pgaptmp_000651"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HGU4557233.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55091..55648
                     /gene="lptA"
                     /locus_tag="ACHOSM_RS03260"
                     /old_locus_tag="pgaptmp_000652"
     CDS             55091..55648
                     /gene="lptA"
                     /locus_tag="ACHOSM_RS03260"
                     /old_locus_tag="pgaptmp_000652"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HGU4557234.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            55655..56380
                     /gene="lptB"
                     /locus_tag="ACHOSM_RS03265"
                     /old_locus_tag="pgaptmp_000653"
     CDS             55655..56380
                     /gene="lptB"
                     /locus_tag="ACHOSM_RS03265"
                     /old_locus_tag="pgaptmp_000653"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HGU4557235.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            56428..57861
                     /gene="rpoN"
                     /locus_tag="ACHOSM_RS03270"
                     /old_locus_tag="pgaptmp_000654"
     CDS             56428..57861
                     /gene="rpoN"
                     /locus_tag="ACHOSM_RS03270"
                     /old_locus_tag="pgaptmp_000654"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HGU4557236.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            57884..58171
                     /gene="hpf"
                     /locus_tag="ACHOSM_RS03275"
                     /old_locus_tag="pgaptmp_000655"
     CDS             57884..58171
                     /gene="hpf"
                     /locus_tag="ACHOSM_RS03275"
                     /old_locus_tag="pgaptmp_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HGU4557237.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            58289..58780
                     /gene="ptsN"
                     /locus_tag="ACHOSM_RS03280"
                     /old_locus_tag="pgaptmp_000656"
     CDS             58289..58780
                     /gene="ptsN"
                     /locus_tag="ACHOSM_RS03280"
                     /old_locus_tag="pgaptmp_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HGU4557238.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            58826..59680
                     /gene="rapZ"
                     /locus_tag="ACHOSM_RS03285"
                     /old_locus_tag="pgaptmp_000657"
     CDS             58826..59680
                     /gene="rapZ"
                     /locus_tag="ACHOSM_RS03285"
                     /old_locus_tag="pgaptmp_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGU4557239.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            59677..59949
                     /gene="npr"
                     /locus_tag="ACHOSM_RS03290"
                     /old_locus_tag="pgaptmp_000658"
     CDS             59677..59949
                     /gene="npr"
                     /locus_tag="ACHOSM_RS03290"
                     /old_locus_tag="pgaptmp_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HGU4557240.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60163..60795
                     /gene="yrbL"
                     /locus_tag="ACHOSM_RS03295"
                     /old_locus_tag="pgaptmp_000659"
     CDS             60163..60795
                     /gene="yrbL"
                     /locus_tag="ACHOSM_RS03295"
                     /old_locus_tag="pgaptmp_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HGU4557241.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKFYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(60792..61520)
                     /gene="mtgA"
                     /locus_tag="ACHOSM_RS03300"
                     /old_locus_tag="pgaptmp_000660"
     CDS             complement(60792..61520)
                     /gene="mtgA"
                     /locus_tag="ACHOSM_RS03300"
                     /old_locus_tag="pgaptmp_000660"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HGU4557242.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAVEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQEWILRQMYQLGGEPFMQQHQLD"
     gene            complement(61517..62170)
                     /gene="elbB"
                     /locus_tag="ACHOSM_RS03305"
                     /old_locus_tag="pgaptmp_000661"
     CDS             complement(61517..62170)
                     /gene="elbB"
                     /locus_tag="ACHOSM_RS03305"
                     /old_locus_tag="pgaptmp_000661"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HGU4557243.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECSVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(62400..64736)
                     /gene="arcB"
                     /locus_tag="ACHOSM_RS03310"
                     /old_locus_tag="pgaptmp_000662"
     CDS             complement(62400..64736)
                     /gene="arcB"
                     /locus_tag="ACHOSM_RS03310"
                     /old_locus_tag="pgaptmp_000662"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HGU4557244.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(64832..65761)
                     /gene="yhcC"
                     /locus_tag="ACHOSM_RS03315"
                     /old_locus_tag="pgaptmp_000663"
     CDS             complement(64832..65761)
                     /gene="yhcC"
                     /locus_tag="ACHOSM_RS03315"
                     /old_locus_tag="pgaptmp_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HGU4557245.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            66436..70896
                     /gene="gltB"
                     /locus_tag="ACHOSM_RS03320"
                     /old_locus_tag="pgaptmp_000664"
     CDS             66436..70896
                     /gene="gltB"
                     /locus_tag="ACHOSM_RS03320"
                     /old_locus_tag="pgaptmp_000664"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HGU4557246.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVNQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            70909..72327
                     /gene="gltD"
                     /locus_tag="ACHOSM_RS03325"
                     /old_locus_tag="pgaptmp_000665"
     CDS             70909..72327
                     /gene="gltD"
                     /locus_tag="ACHOSM_RS03325"
                     /old_locus_tag="pgaptmp_000665"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HGU4557247.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            72511..72915
                     /locus_tag="ACHOSM_RS03330"
                     /old_locus_tag="pgaptmp_000666"
                     /pseudo
     CDS             72511..72915
                     /locus_tag="ACHOSM_RS03330"
                     /old_locus_tag="pgaptmp_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            73007..73312
                     /locus_tag="ACHOSM_RS03335"
                     /old_locus_tag="pgaptmp_000667"
     CDS             73007..73312
                     /locus_tag="ACHOSM_RS03335"
                     /old_locus_tag="pgaptmp_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4557248.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(73388..73852)
                     /gene="nanQ"
                     /locus_tag="ACHOSM_RS03340"
                     /old_locus_tag="pgaptmp_000668"
     CDS             complement(73388..73852)
                     /gene="nanQ"
                     /locus_tag="ACHOSM_RS03340"
                     /old_locus_tag="pgaptmp_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HGU4557249.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(73849..74724)
                     /gene="nanK"
                     /locus_tag="ACHOSM_RS03345"
                     /old_locus_tag="pgaptmp_000669"
     CDS             complement(73849..74724)
                     /gene="nanK"
                     /locus_tag="ACHOSM_RS03345"
                     /old_locus_tag="pgaptmp_000669"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HGU4557250.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTDLPTIA
                     INDAQAAAWAEYQALEGDVSEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQALQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(74721..75410)
                     /gene="nanE"
                     /locus_tag="ACHOSM_RS03350"
                     /old_locus_tag="pgaptmp_000670"
     CDS             complement(74721..75410)
                     /gene="nanE"
                     /locus_tag="ACHOSM_RS03350"
                     /old_locus_tag="pgaptmp_000670"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGU4557251.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAER
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(75458..76948)
                     /gene="nanT"
                     /locus_tag="ACHOSM_RS03355"
                     /old_locus_tag="pgaptmp_000671"
     CDS             complement(75458..76948)
                     /gene="nanT"
                     /locus_tag="ACHOSM_RS03355"
                     /old_locus_tag="pgaptmp_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HGU4557252.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(77057..77950)
                     /gene="nanA"
                     /locus_tag="ACHOSM_RS03360"
                     /old_locus_tag="pgaptmp_000672"
     CDS             complement(77057..77950)
                     /gene="nanA"
                     /locus_tag="ACHOSM_RS03360"
                     /old_locus_tag="pgaptmp_000672"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGU4557253.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVILPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(78072..78863)
                     /gene="nanR"
                     /locus_tag="ACHOSM_RS03365"
                     /old_locus_tag="pgaptmp_000673"
     CDS             complement(78072..78863)
                     /gene="nanR"
                     /locus_tag="ACHOSM_RS03365"
                     /old_locus_tag="pgaptmp_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HGU4557254.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            complement(78841..>79062)
                     /locus_tag="ACHOSM_RS03370"
                     /old_locus_tag="pgaptmp_000674"
     CDS             complement(78841..>79062)
                     /locus_tag="ACHOSM_RS03370"
                     /old_locus_tag="pgaptmp_000674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557255.1"
                     /translation="FIMLVNSLINNSIGMFLSALHYFFAIVNYSVNYGNDEAQRENPA
                     IFPYFQSCDRMSLMMLITLTLQNGPYERI"
BASE COUNT        19503 a        21341 c        19609 g        18609 t
ORIGIN      
        1 aatatgatta tgtacttgtt acaaggataa ggttataaat gaataaagtt acaaaaacag
       61 ctattgtggg cttacttgcg cttttcgcag gtaatgccgc tgcaaccgat ggtgaaatag
      121 tttttgatgg cgagatttta aaatccgctt gtgaaatcaa tgactctgat aagaaaattg
      181 aagttgctct tggtcactat aatgccgaac agtttcgttc tgttggcgat cgtagcccaa
      241 aaattccatt cactattcct ttagttaact gcccagtgac tgggtgggag cacgataacg
      301 gcaacgttga agcgtctttc cgtttatggc tggaaacgcg ggataatggc accgtcccta
      361 acttccctaa tctggcaaaa gtgggctctt ttgctggcac agcggcaaca ggtgtgggta
      421 tccgtattga cgatgcggaa agcggaaacc ttatgccact gaatgctatg gggaatgata
      481 acacggttta tcagatccca gcggactcca atggtattgt caatgttgac ctcatcgctt
      541 actacgtatc aaccgttgaa gcatcagaaa tcaccccagg ggaagcagac gctgtcgtta
      601 acgtaacgct ggattaccgt taatacgtta cggcgttatc tgacctttca gataacgccc
      661 ttttccttcc tctttctcgt tgtatcaggt tgaaaaataa ctatgtcaaa acgaacattc
      721 gcggtgataa taaccttgtt gtgtagcttc tgtatcggcc aggcgcttgc agggggtatc
      781 gttttacagc gaacgcgagt gatctatgat gccagccgca aagaggctgc gttacctgtc
      841 gcaaacaaag gcgcagaaac gccttattta ctgcaatcat gggtagataa tatagatggt
      901 aaaagccgtg ccccatttat tataacccca ccgctatttc gtcttgaggc tggcgatgac
      961 tcatcactgc gaattattaa aacagctgat aacctgcctg aaaataaaga gtcgctgttc
     1021 tacattaatg ttcgtgccat tccagcaaag aaaaaatcag atgatgttaa tgctaacgag
     1081 ttgacgctgg tatttaaaac acggatcaaa atgttttatc gccccgcaca cctgaaggga
     1141 cgggtaaacg atgcgtggac atcactggaa tttaaacgta gtgaccattc actcaatata
     1201 tataacccaa ctgaatatta cgtcgtattt gccggactgg cagtcgataa aaccgatctc
     1261 acaagcaaaa ttgaatatat cgcgcccgga gaacataaac agttaccact tcctgcatct
     1321 ggcggaaaga acgtgaaatg ggctgcaatc aatgattatg gcggcagttc cgggacagaa
     1381 actcgtccac tgcaataaca aatataaaaa acacaggtca tcagggaatg ccacaacgac
     1441 accaccaggg acataaacgc acaccgaaac agttggcgct catcatcaaa cgctgtttgc
     1501 cgatggtgct cactggcagc ggcatgcttt gcactaccgc taacgccgaa gagtattatt
     1561 tcgaccccat tatgctggaa actacaaaaa gtggtatgca aacaactgat ctgtcacgtt
     1621 tttcaaaaaa atacgcacaa ctaccaggaa cttatcaggt tgatatctgg ctgaataaaa
     1681 agaaggtttc acagaaaaaa attacattta ccgccaatgc agagcaactt ctgcagccac
     1741 agtttacggt agaacaacta cgtgagctgg gtattaaggt ggatgaaatc ccggcgctgg
     1801 ctgaaaaaga tgacgatagc gtgatcaact cgcttgaaca aatcattccc ggtacagctg
     1861 ctgaatttga tttcaatcat cagcgactta atttgagcat tccccaaatt gcactgtacc
     1921 gtgatgcaag aggttacgtc tccccttctc gttgggacga tggtatacca acgctgttta
     1981 ccaactactc gtttacaggt tctgataacc gttaccgcca gggcaatcgt agccaacgac
     2041 agtacctgaa tatgcaaaat ggtgctaatt ttggcccctg gcgattacgc aactattcca
     2101 catggacacg caacgatcag acatcaagct ggaataccat cagtagttat ttacaacgtg
     2161 atatcaaggc attgaagtct cagttgcttc tgggagaaag cgccaccagc ggcagtattt
     2221 tttccagcta cacctttact ggcgttcaac tcgcttccga cgataatatg ctgccaaaca
     2281 gccagcgcgg atttgcccca acggtacgcg gtatcgcaaa cagtagtgca atcgtgacta
     2341 tcaggcaaaa tggttatgtg atctatcaaa gcaacgtgcc agcgggtgtc tttgaaatta
     2401 acgatctcta cccctcttcc aacagcggcg atttagaagt cacgattgaa gaaagtgacg
     2461 gtacgcaacg tcgctttatc cagccttatt cttcattacc catgatgcag cgacctgggc
     2521 atctaaaata tagcgcgacc gctggacgct atcgcgctga tgcaaacagt gatagcaaag
     2581 aacccgaatt tgctgaagcc acggcaatat atggtttgaa taatactttt acgctgtatg
     2641 gtggcctgct cggttctgaa gattattatg cgctggggat cggtatcggc ggcacacttg
     2701 gcgcactggg cgcgttgtcg atggatatca acagagctga cacccaattc gataaccagc
     2761 actcttttca tggctatcaa tggcgtacgc agtacatcaa agatatcccg gaaaccaaca
     2821 ccaatatcgc tgtcagctac tatcgctata ccaacgatgg ctattttagt tttaatgaag
     2881 ccaatacccg taattggaac tataacagtc gccaaaaaag tgaaattcaa ttcaacatca
     2941 gccagacaat atttgatggg gtaagtctgt atgcctccgg ttcgcagcaa gactattggg
     3001 gcaataacga taaaaacagg aatatctcag ttggggtttc cggccagcaa tggggaattg
     3061 gttacagcct gaattatcaa tacagtcgct acacggatca aaataatgac cgcgcactct
     3121 ctttgaatct cattattccg ttagaacgct ggttaccgcg tagccgggtt tcctatcaga
     3181 tgaccagcca gaaagatcgc ccaacccaac atgaaatgcg tcttgatggc tcactgctgg
     3241 atgatggtcg cctgagctat agcctggaac aaagtctgga tgacgataac aaccataaca
     3301 gtagcctgaa cgccagttac cgttcacctt atggcacctt cagtgccgga tacagttacg
     3361 gtaatgacag cagccaatac aattacggcg ttaccggcgg cgtggttatc catcctcatg
     3421 gcgtgacgct ctcgcaatat ctgggcaacg cttttgcgct tatcgatgct aatggggctt
     3481 ctggcgtgag gatacaaaac tatccgggga ttgctactga tccctttggc tatgcagtgg
     3541 ttccttatct cacgacttac caggaaaacc gtctctcggt agatactacg cagctgcccg
     3601 ataacgtcga tcttgagcaa acaacacagt ttgtggtgcc caacagaggt gcaatggtag
     3661 cggcgcgttt caacgccaat atcggttatc gcgtacttgt tacagtcagc gatcgcaacg
     3721 gtaaaccgtt gccctttggc gctcttgcca gcaacgatga gacggggcaa caaagtatcg
     3781 tcgatgaggg cggcatacta tatctctctg ggatatcgag taaatcacaa agctggactg
     3841 tacgctgggg aaatcaggca gatcaacaat gtcagtttgc ttttagtaca ccggattcag
     3901 aaccaacaac ctctgtatta caaggcacgg cgcagtgcca ttaaggataa aaaaatgaaa
     3961 agagcgcctc ttataacagg acttttgttg atatccacat cctgcgctta tgcctcctca
     4021 ggagggtgtg gagctgacag cactagcggt gcgacaaatt acagcagtgt ggttgatgat
     4081 gttacggtga accagacaga taacgtgaca ggacgggagt ttacctctgc cacgctaagt
     4141 agcactaact ggcaatacgc ctgtacctgc tctgcgggta aggcagttaa acttgtctat
     4201 atggtcagcc ctgtacttac caccactgga catcaggcag gatattacaa actcaatgat
     4261 agtctggata ttaaaaccac attacaggcg aatgacattc caggactcgt gactgaccag
     4321 accgtttctg ttaacacccg attcacacag ataaaaagca acaccgtata ttctgctgca
     4381 acccaaacgg gtgtttgcca gggtgacacg tctcgttatg gacccgttaa tattggtgca
     4441 aataccacct ttaccctgta tgtcaccaag ccatttctcg gctcgatgac cattccgaaa
     4501 acggatattg ccgtcattaa aggcgcatgg gtcgatgaaa tgggaagccc gtctacaggt
     4561 gacttccatg atttagtcaa gttatcgatt cagggaaatc tcaccgcccc acagtcgtgc
     4621 aaaattaatc agggcgatgt tattaaggtt aattttggat tcatcaatgg tcagaagttt
     4681 accacccgca atgccatgcc agacggtttt actccagtag actttgatat cacttatgac
     4741 tgtggtgata cttcaaagat taaaaactcg ttgcaaatgc gcatcgacgg tacaactggg
     4801 gtagtagacc agtacaacct ggtcgccaga cgaagaagtt cagacaatgc gcccgatgtc
     4861 ggtattcgta ttgaaaatct cggcggcgga gttgcaaata ttccttttca gaacggtatc
     4921 cttcccgttg atccttccgg gcatggcacc atcaacatgc gcgcctggcc agttaatctg
     4981 gtcggtggtg agctggaaac aggaaaattt cagggcaccg ccaccattac cgtcatcgtg
     5041 cggtaaaaga agacgttgcg ttttgattaa cgcaacgctt cggcactctt acccctgctg
     5101 ctccagcgca tacttataca gcgcattttt cttcacgccg tgaatttctg ccgccagcgc
     5161 cgccgctttt ttcaacggca gttctgcctg tagcagcgcc agcgtgcgca gggcatcggc
     5221 gggtaagtct tcttcctgtg ctttatgacc ttcgataatc agcaccattt cgcctttgcg
     5281 acggttttca tcttctttta cccacgccag cagctcgcca acgggcgcgc cgtgaatggt
     5341 ttcccaggtt ttggtcagct cacgcgccag aaccacgtag cgggattcgc ctaataccgc
     5401 aacgatatct tccaggctat ctaacagacg gtgggtagat tcataaaaaa tcagcgtgcg
     5461 cggctccgct tcaatggctt ttagcgcatc acggcggcct tttgatttgg caggtaaaaa
     5521 gccttcgtaa cagaaacggt cagagggtaa acccgcggcg cttaacgcag tgatagcagc
     5581 acacggtccg ggtagcggca ccacacgaat ccccgcttca cggcaggtac gcaccagatg
     5641 gtagccagga tcgttaatta gcggcgttcc ggcatcggaa accagcgcaa tgttttgccc
     5701 ctcttgcagc ttcgccagca gcgtttcggc tttttgttgt tcgttatggt cgtgcagcgc
     5761 aaacaaccgg gcattaatcc caaaatgttg cagcaataaa ccggtgtgac gagtatcctc
     5821 ggcggcaatc agctcaacgg cctgtaatac ctctaacgca cgctgggtga tatccgccag
     5881 attgccgatt ggcgtcggta caatgtaaag ctggccctga gaattatccg ccgattggtg
     5941 ttgtttcatt gtgtcgtccg tattgccgat ttaatattga gcattgcgta aaaaaatatc
     6001 actggataca ttatggtacc ctcaacattt tctcgtttga aagccgcgcg ttgtctgcct
     6061 gttgttctgg cagccctgat tttcgccggt tgtggcaccc atactcccga tcagtccact
     6121 gcttatatgc agggcacggc gcaggctgat tctgcctttt atcttcagca gatgcagcaa
     6181 agctctgatg ataccaggat caactggcaa ttactcgcca ttcgtgcact ggtgaaagaa
     6241 ggtaaaaccg ggcaggcggt tgagttgttt aaccaactac cgcaagaact gaacgattct
     6301 cagcgtcgcg agaaaacact gctggcggta gagattaaac tggcgcagaa agattttgct
     6361 ggcgcgcaaa acttgctggc gaaaattaca cccgccgatt tagaacaaaa ccagcaagcg
     6421 cgttactggc aggcaaaaat cgatgccagc caggggcgtc cttccattga tttactgcgc
     6481 gcgttaattg ctcaggaacc gctgcttggc gcgaaagaaa aacagcagaa tattgatgcc
     6541 acctggcagg cgctctcctc catgactcag gaacaggcga atacgctggt gatcaacgcc
     6601 gacgaaaata ttctgcaagg ctggctggat ctgcagcgcg tctggtttga taaccgtaac
     6661 gatcccgaca tgatgaaagc cgggatcgcc gactggcaga aacgttatcc gaacaacccg
     6721 ggcgcgaaaa tgctgccaac gcagttggtt aacgtaaaag cgtttaaacc agcatcaacc
     6781 aacaaaatcg ccctgctgtt gccactgaat ggccaggcag cggtatttgg tcgcactatt
     6841 cagcaaggct ttgaagcggc gaaaaatatc ggcactcagc cagtggcagc tcaggtagct
     6901 gccgcacctg ccgcagacgt agcagaacaa cctcaaccgc aaaccgtgga tggcgttgcc
     6961 agcccggcac aagcctcggt tagcgatctg accggtgaac agcctgcagc ccagccggtg
     7021 cctgtaagcg ccccggcgac aagcaccgca gcggtaagcg cacccgcaaa tccatccgca
     7081 gagctgaaaa tctacgatac ctcatcacaa ccacttagcc agatcttaag ccaggttcag
     7141 caggatggcg cgagtattgt ggtcggtccg ttgctgaaaa ataacgttga agagttgctg
     7201 aagagcaaca ctccgctgaa cgtactggca ctgaaccagc cggagaatat cgaaaatcgc
     7261 gtcaatattt gttacttcgc gctttcaccg gaagacgaag cgcgcgatgc agcgcgtcat
     7321 attcgtgacc agggtaaaca agcgccgcta gtgctgatcc cacgcagttc attgggcgat
     7381 cgcgtagcca atgcgtttgc gcaagagtgg cagaaactgg gcggcggcac cgttctgcaa
     7441 caaaaatttg gttccaccag cgaattacgc gcgggtgtta acggcggttc tggtatcgct
     7501 ttaacgggta ccccgattac tctcagagcg acaaccgact ccggcatgac gaccaacaat
     7561 ccaacgctgc aaaccacgcc aaccgatgac cagttcacca ataatggcgg tcgtgtcgat
     7621 gcggtgtaca ttgtggcaac gccgggtgaa atcgctttta tcaaaccgat gatcgccatg
     7681 cgtaacggta gccagagcgg tgcaacgctg tacgccagct cccgcagtgc gcaagggacc
     7741 gctggcccgg atttccgact ggagatggaa ggcttgcagt acagcgaaat cccgatgctg
     7801 gcaggcggta atctaccgtt aatgcagcag gcactcagcg cggtgaataa cgattattca
     7861 ctggctcgca tgtatgcgat gggcgtcgat gcctggtcgc tggcaaatca tttctcacaa
     7921 atgcgccagg ttcagggttt tgaaatcaac ggtaataccg gaagcctgac ggctaacccg
     7981 gattgcgtga ttaacaggaa gttatcatgg ctacagtacc aacaaggtca ggtagtcccc
     8041 gccagttaac cactaaacag accggcgatg tgtgggaagc acaagcgcgt cgctggctgg
     8101 aaggcaaagg actgcggttt atcgccgcta acgtgaacga gcgtggcggc gagatcgatc
     8161 tgataatgcg tgaaggccgg accaccgttt ttgtcgaggt acgctatcgc cgctctgcgc
     8221 tttatggcgg cgcggcagcc agtgtgaccc gcagcaaaca acacaaatta ttacagactg
     8281 cccgcttgtg gctcgcgcgt cataatggga gttttgatac tgtggattgc cggttcgatg
     8341 tggtagcctt caccgggaat gaggttgagt ggattaagga tgcctttaat gaccactcat
     8401 aattaaggtt taaggattag cgtgcaagaa agaattaaag cttgcttcac tgaaagcatt
     8461 caaactcaaa ttgcggcggc agaggcgctt ccggatgcca tctcccgtgc agccatgacg
     8521 ctggttcagt ctctgctcaa tggcaacaaa atcctctgtt gtggtaatgg aacttccgct
     8581 gccaatgcac agcattttgc tgccagcatg atcaaccgtt tcgaaacgga gcggcccagc
     8641 ttacctgcca ttgcactaaa tactgataat gttgtcttaa cggcgattgc caacgatcgc
     8701 ttacatgatg aagtgtatgc aaaacaggtg cgggcgctgg gtcatgcggg agatgtattg
     8761 ttagccattt ccacccgtgg caacagccgc gatattgtaa aagcggtaga agctgccgtt
     8821 acgcgtgata tgaccattgt ggcattgacc ggctatgacg gcggtgaact tgcaggtttg
     8881 ttagggccac aggatgtgga gatccgcatt ccttcgcatc gtagtgctcg cattcaggaa
     8941 atgcatatgc tgacggtaaa ttgcctgtgc gatctgatcg ataacacgct tttccctcac
     9001 caggatgatt aaggagaata catgaaggca ttatcgccaa tcgcagtcct tatttccgcg
     9061 ctgctgttgc aaggttgtgt tgccgctgcc gtagtgggta ccgctgctgt gggtaccaaa
     9121 gccgcaactg acccacgcag tgtcggcacc caggtggacg atggtaccct ggaagtgcgc
     9181 gtgaacagcg cattgtcgaa agacgaacag attaagaaag aagcgcgcat taatgtaacg
     9241 gcctatcagg gcaaagtgct gctggttggg cagtcaccaa acgctgaact ttcggctcgc
     9301 gctaaacaga ttgctatggg cgtagacggt gccaacgaag tgtataacga gattcgtcag
     9361 ggccagccga ttggtctggg cgaagcgtct aacgatacgt ggatcaccac caaagtgcgt
     9421 tcgcagctct taaccagcga cttggtgaaa tcgtccaacg tgaaagtgac caccgaaaac
     9481 ggtgaagtgt tcctgatggg gctggtgact gaacgtgaag cgaaagcggc ggcagatatt
     9541 gccagccggg tgagcggcgt gaagcgggta actacggcgt ttacgtttat taaatagcag
     9601 cccttgtaat gcctgatgcg acgcttgccg cgtcttatca ggccaacaac gatgcaaacc
     9661 gtaggtcgga taaggcgttt acgccgcatc cgacatgttt tccctcaaat tagaacaaca
     9721 gcgccagccc gccgacaatc acgcctgaca ctgccaccat cgcccccgtc agccataagg
     9781 ctttcgccgg gaacgcattg cgcagcataa ccagtgacgg caaactcacc gccgggagcg
     9841 tcatcaacaa tgccagcgcc ggagcagttc ccatacctgc cagcatcatc gtttgtacaa
     9901 tcggaatttc tgctgccgtg ggaatgacaa acaagcatcc tgctaccgcc atcgccacca
     9961 cccacatcag gctgttatcg acagcaccat cggcatgggg gaataaccag acgcgagcgg
    10021 cacccaacac cagtactgca aggatgtaaa ccgggatcgt actccagaaa agcgtccata
    10081 gcgccctgcc ccagcggcta aaaaaaccgc cctgtgcttc cggtatgtca atttcgaccg
    10141 gtgcctgcgt ttgcggtgtt tcacgcaccc atttttgcac cagcgtcgca atcagcaaca
    10201 ccatcaccag cccggccacc agacgaatcg ccgcaaaacc ccagccgagg acaaacccca
    10261 taaacaccag cgtcgccggg tttaacaccg gattgcccat ccagaatgcc agcgcaccgc
    10321 ccatcgacac ctgctggcga cgcatccctg ccgcgaccgg agccgcacag caggtacaca
    10381 tcatgcccgg cagcgaaaac agcgttccca gcagcgtgcc gcgaaagcgc gattgcccaa
    10441 gcgtacgcaa caaccagtca cgcgggatca gcacctgaat caacgaaccg aggatcaccc
    10501 ccagcaccgc cgctttccat accgcgagga aatagatcat cgcgtaatcc aacgccgcct
    10561 gccacggatt agcatccgcc tgcgcaagga tagatttacc gatactgtgg gtttcggcag
    10621 cagtaaacgc tttgccgtag taaggttgcc atttcacata ccagagaccg gcaatgacaa
    10681 cgagaaagaa aagcgcgggt ttccaccact gaatgggcgt tgccgcctga gatgaagact
    10741 gaccagtcat agcattcccc aggaataagt tatgataatt gagcgcgtga atattacgct
    10801 cactatcaat tcttgggaat aattattcag ctcttttgcg taattctgaa gagcttaaaa
    10861 tggtcacgcc ttcatgctct ggccgcatcg cttctgccag catgacccgc gccacgtctc
    10921 tcgcatcaat ggatttccag ttaccgggta acaaacggaa cagcggcgca aaaagcgttt
    10981 cgttcatccg ctgtttgcta cgatcgccca gtaacatcga cgggcgcgca atggtcaatt
    11041 tcggccagtt ctgggcaatt aatgcttctt ccatctcccc tttgacgcgg ttatagaaaa
    11101 acggcgagtg ggcattggca cccatagcac tgaccaccag catatgctgc gcgcccagcc
    11161 gccgcccggt taatgcggta tccactacca gcgtgtaatc ggcatgaata aacgcctctt
    11221 tgctccccgc ttctcgccgc gtggtgccga gacaacaaaa cacaatgtcg atgggatcgg
    11281 tgacctgcgc caacgcgtcg gtcagttgcg gatcatgggg attgaacacc ccgggcatat
    11341 cgcccaacgg acgtcgcgtc ggcgcggcta tggcgttaac tttcggttcg ttaatcaaca
    11401 tccgcagcag gtgaccgccc accagccccg ttgcgcctgt aatcagtacc tgactcatct
    11461 tcgctccttt acagaattgt ctgccttgcg ctccccggct catgcaccag gcttaataga
    11521 ccatgaggta attatccccg attgtgggaa attcgcctca tccaatgcaa caacgcggag
    11581 gaagcatgag taagaaaatt gccgttttaa tcactgatga atttgaggat tcagaattta
    11641 cttcacccgc agacgagttc cgtaaagccg gacacgaagt gattaccatt gaaaaacaag
    11701 cgggtaaaac ggtgaaaggc aaaaaaggag aagccagcgt gaccatcgat aaatccatcg
    11761 atgaagtgac gccagcggag tttgatgccc tgctgctacc gggcggccat tcaccggatt
    11821 atctgcgtgg cgacgaccgt tttgttacct ttacccgtga ttttgtgaat agcggcaaac
    11881 ctgttttcgc catctgtcac ggcccgcagc tgctgatcag cgccgatgtg attcggggac
    11941 gcaaactgac cgcggttaaa ccgatcatta ttgatgttaa aaacgcgggc gcggaatttt
    12001 acgatcagga agtcgtggtc gataaagatc agctggttac cagccggaca ccggacgatc
    12061 tgccagcgtt taaccgcgag gcgttacgcc tgctcggtgc ctgagccgcg tagccaaatc
    12121 atttttttac caaagcccag cgtgttgtcg gtgaatttga tttcatcaag ccggatttcc
    12181 cacaccgggg ccgacagcat tctggcaacc ggaaagcgac gattgtacgc cttgcgcgcg
    12241 aggtcgcttt cctcaccttc cagcctgcgg atctcacctt taaactgcac gccgcgaatc
    12301 aacgctaccg ttttcggctg accgtttacc gttccggcaa ccgcagcctg cggcccactc
    12361 atctgcgcgt ggcgcgtttt ttcttccgtc aaaatgtaga aggcgacttt ctgcgcatca
    12421 aaaagataaa aggcattagc gcaccacagt tccccttcct gctgcacaca ccaggtgaca
    12481 acatgttgtt ttgccagcca acggctgatg gcgatgagtg tttccattgc tgttctcttt
    12541 tatactgtgg gcctgacctt aacatgcgat gcagacgatg acaccctggt ttctttactt
    12601 gatccgtacc accgacaata agctttatac cgggatcacc acggatgtcg aacgccgcta
    12661 tcagcagcac caaagcggca aaggggcgaa agcactgcgc gggaaaggtg aactgacgct
    12721 ggcgttttcc gcaccagtgg gcgaccgttc gctagcgtta cgggcggaat atcgcgttaa
    12781 acaactgacc aaactgcaaa aagagcgtct ggtagcggaa ggcgcagggt ttgcagagct
    12841 gttaagcagt ctgcaaaccc cggagattaa aagcgattga aatgctcgtg atactcaacc
    12901 agaccggtta cgccattcag cgcgtcatcc gctaaacgat gtacctggaa ggcgctttca
    12961 gtgcccggcc agcggcaacg cagatcgtga tgtgccgcca gttcaaagcc gaaacggctg
    13021 tacagcgccg gatcgcccag cgtcaccact gcggcatagc cgaactcatt aagcgaatcg
    13081 agtccttcat agaccagttg gcgtgccagc ccctgtccac ggtatttttc atctaccgcc
    13141 agcggtgcca tgccgaccca ttgcaggtct tcgccctgca catcaaccgg actaaatgcc
    13201 acatagccaa tgacctgacc ttcgtcatct gtcgccacca gccccagcgt cagaaagcct
    13261 tcttcacgca gatcgtgaac cagcttcgct tccgcatcac tttcgaatga acgacgcagc
    13321 agggcatcaa tacccggcgc atcaatggga atttctactc gaattagcat ggttcaccta
    13381 ccgatgtctg tttggtttca ggcgcggttt tcattcccgc ctcaacaaaa tccgccagtt
    13441 gcagcagcat catgcgtaag gctttcggca tttgctccag ttcaatagcg tccatcaggt
    13501 ttttcacata cagccccagc tccgtatcgc cttcaatcac cagccgacgc tggaagaaga
    13561 gcgtatccgg atcttgttta cgcgccgcaa tcatcagcag atcgctggcg tcggcactaa
    13621 aactcacatc agcttgcgcg ttctggctga ccaccagttt gccattcacc accgaggtaa
    13681 accattgcag gtcaatatca cgcacatgaa tacttaacct gcggccttca agaaactcca
    13741 gctcaccatc atccagcgcc tggcggaatt gccagcttaa gacctgctca agaacctggc
    13801 gttttagcgc aaatggcgtc agttttaccg gtacactcaa cagagatggc cccaaatgca
    13861 caatacggga acgcagttta tccaacacga gttttactcc ctgtttcaac aatcatccta
    13921 ttttgccata tcagaaaaat aacatagcgg tataaatcaa caattccaca tgaaattgct
    13981 gctaccacca atacaacttt aactgcctta aatcaaaaat tgtcgcagca aggttaacta
    14041 aaatcccagt tcgttaacat ttttgcgttt tgatagcgca accttcagga aaaattatgg
    14101 agctgctctg ccctgccgga aatctcccgg cgcttaaggc ggccatcgaa aacagcgcag
    14161 atgctgttta tatcgggcta aaagatgata ccaatgcccg tcacttcgcc ggccttaact
    14221 ttaccgagaa aaaattgcag gaagcggtga gttttgtcca tcaacatcgc cgcaaacttc
    14281 acatcgcgat taacactttt gcgcatccgg acggttacgc ccgttggcag cgtgccgtgg
    14341 atatggcggc gcagctgggt gccgacgcgc tgatcctcgc cgacctcgcc atgctggagt
    14401 acgccgccga gcgttacccg catattgagc gtcatgtgtc agtgcaggct tcggcgacca
    14461 atgaagaggc gattaacttt tatcatcgcc attttgacgt tgctcgcgtg gtgctgccgc
    14521 gcgtgttgtc gattcatcag gtgaaacaac tggcacgggt cacacctgta ccactggaag
    14581 tgtttgcttt cggcagcctg tgcattatgt cggaaggccg ttgctatctg tcgtcgtatc
    14641 tgacggggga atcgcccaac accgtgggcg cgtgttctcc ggcccgtttc gtgcgctggc
    14701 agcaaacgcc gcaggggctg gaatcccgcc tgaacgaagt gctgatcgac cgttatcagg
    14761 acggcgaaaa cgcaggttat ccgacgctgt gtaaagggcg ttatctggtg gacggcgagc
    14821 gctatcacgc gctggaagaa ccaaccagtc tcaataccct ggaactgctg ccggagttaa
    14881 tggcggcgaa tattgcttcg gtgaaaattg aaggccgcca gcgtagcccg gcgtatgtca
    14941 gccaggtggc gaaagtctgg cgtcaggcta tcgaccgttg taaggccgat ccgcaaaact
    15001 tcgtaccgca aagcgcgtgg atggagacgc tcgggtcgat gtccgaaggc acgcagacca
    15061 cccttggcgc gtatcaccgt aaatggcagt gagaagagca atgaaatatt ccttagggcc
    15121 agtgctgtgg tactggccaa aagaggcgct ggaagaattt tatcagcagg ccgccaccag
    15181 cagcgccgac gtgatttatc ttggtgaagc ggtatgcagc aagcgccggg caaccaaagt
    15241 tggcgactgg ctggagatgg caaaatcgct cgccggaagc gggaagcaga tagtgctctc
    15301 cacgctggcg ctggtgcagg catcctctga actgggcgaa ctgaaacgct atgttgaaaa
    15361 cggtgagttt ctgattgaag ccagcgatct cggcgtggtg aatatgtgcg ctgaacgcaa
    15421 actgccgttc gtcgccgggc acgcgctgaa ctgctacaac gcggtgacgc tgaaaatatt
    15481 gctcaaacag ggcatgatgc gctggtgtat gccggtggag ctttcccgcg actggctggt
    15541 gaatctgctt aatcagtgcg atgagttggg gattcgcaac cagtttgaag tggaagtcct
    15601 gagctacggt catctaccgc tggcctactc cgcccgctgc tttaccgcgc gttcggaaga
    15661 ccgcccgaaa gatgagtgtg aaacctgctg cattaagtat ccgaacgggc gcaacgtgct
    15721 gtcgcaggaa aaccaacaag tgtttgtact caatggcatt cagaccatga gcggctacgt
    15781 ttacaacctc ggtaacgaga tgacatccat gcagggattg gttgatgtgg tccgcctgtc
    15841 accgcagggt actgacactt tcgcgatgct cgacgccttc cgcgctaatg aaaatggcgc
    15901 tgcgccactg ccgttgacgg caaacagcga ctgtaacggc tactggcggc ggctggctgg
    15961 gctggagctg caagcctaag taaatatgct cgtcatactt caagttgcat gtgctgcatc
    16021 tgcattcgtt caccccagtc acttacttat gtaagctcct ggggattcac tctcttgtcg
    16081 ccttcctgca actcgaatta tttagagcac agctcacttt gttaacaact ttaactactc
    16141 tttaatgcag tattaaagat taatcggtaa caaagtgagc tgttatgact gataaaacca
    16201 ttgcgttttc gctactcgat ctggccccca ttcccgaagg ttcttcagcg cgagaagcat
    16261 tctcccactc tctcgatctc gcccgtctgg ctgaaaagcg cggctatcat cgctactggc
    16321 tggcagaaca ccacaatatg actggcattg ccagtgctgc cacgtcggta ttgattggct
    16381 atctggcggc gaataccacc acgctgcatc tggggtctgg cggcgtgatg ttgcctaacc
    16441 actcaccgtt ggtcattgcc gaacagttcg gcacgctcaa tacactctat ccggggcgaa
    16501 tcgatttggg gctgggtcgt gcgccaggta gtgaccaacg gacaatgatg gcgctgcgtc
    16561 gccatatgag cggcgatatt gataatttcc cccgcgatgt cgcggagctg gtggactggt
    16621 ttgacgcccg cgatcccaat ccgcatgtgc gcccggtacc aggctatggc gaaaaaatcc
    16681 ccgtgtggtt gttaggctcc agcctttaca gcgcgcaact ggcggcgcag cttggtctgc
    16741 cgtttgcgtt tgcctcacac ttcgcgccgg atatgttgtt ccaggcgctg catctttatc
    16801 gcagcaactt caaaccgtcg gctcgactgg aaaaaccata cgcgatggtg tgcatcaata
    16861 ttatcgccgc cgatagcaac cgcgacgctg agtttctgtt tacctcaatg cagcaagcct
    16921 ttgtgaagtt gcgccgtggc gaaaccgggc aactgccgcc accgattcaa aatatggatc
    16981 agttctggtc gccgtccgaa cagtatggtg tgcagcaggc gctgagtatg tcgttggtag
    17041 gtgataaagc gaaagtgcgt catggcttgc agtcgatcct gcgcgaaacc gacgccgatg
    17101 agattatggt caacgggcag attttcgacc accaggcgcg gctgcattcg tttgagctgg
    17161 cgatggatgt taaggaagag ttgttgggat agtgtgtctt aacgcgggaa gccttatccg
    17221 agctggcaac gctgtcctac gtagacctga taagcgaagc gcatcaggca ttgtgtaggc
    17281 agcagaaatg tcggataagg caccgctgat tactgataca ccggcagtaa attaaagctc
    17341 gataaaatat ggaccagtgc gttgccaacg ccaaacacca gaatcagcgt aatcatcggc
    17401 ttgccgcccc agacgcagaa tttcgggctg ccaaagcgtt tacgcgatgc gcgggctaac
    17461 agcgccggaa caattgccgc ccagatggta gctgctaaac cagcataacc aatggcgtac
    17521 aggaatccgt tcgggaacag tagaccaccc acaacaggcg gggcaaaggt cagcaatgcc
    17581 gttttcaagc gccccagggc tgagtcatcg aaaccaaaca gatctgccag atagtcaaac
    17641 aaacccagcg ttacgccaag gaacgaactc gctaccgcaa agtttgagaa cacgaccagc
    17701 agaaggtcca ggctacggct gttcagcacg ccgcttaatg cctgtaccag cacatcaata
    17761 ttaccgccct tctctgcaat accgataaac tccggacgcg ggatgttacc catcgtcgcc
    17821 agcaaccaga tggtatacag cgccagtgcc atcagcgtac cgtacaccag gcatttcacg
    17881 atggttttcg gatctttgcc gtaatacttc atcaggcttg gcacgttacc gtgataacca
    17941 aacgacgcca ggcagaacgg caatgtcatc agcagatacg gcgcataaga tgcattgctt
    18001 tctgcaacgt tgaacaatgt cgcaggctgc acatgcccca acaggctgcc aaaggtgagg
    18061 aagaaggtaa tgactttcgc ccccagcaca atcgccgtca tgcggctgac ggccttagta
    18121 ctcaaccaca ccacaaacgc taccagcagt gcaaaaccaa aacccgccgc ccgtgccggg
    18181 acgtttagcg acatctctgc gaaggtgtga tgcagaattg aaccactggc agaaatatag
    18241 gcataggtca ggatatagag cacaaaggca atggaaatac cgttgaccac gttccagcct
    18301 ttgcccagca aatctttggt gatggtgtca aaactcgaac cgattcggta attcaggtta
    18361 gcttccagaa tcatcaagcc ggaatgcagc atacagaacc aggtaaagat cagcgccgcc
    18421 attgaccaga aaaaccacgc cccggacatg accactggca gagaaaacat ccctgcgcca
    18481 ataatggtgc cgccgataat caccacgccg ccaagcagcg acggtgacgt ttgggtggtg
    18541 gttagtgttg ccatgagggc ttctctccag tgaaaaatag tgcgactgcg ttgttatgca
    18601 ttgcactgta ccagtacacg agtacaaaag acagaaaaaa agccccgatg gtaaaaatcg
    18661 gggctgtata tattatttta cagattgtgt tcgctgttca gcgatgatta cgcatcacca
    18721 ccgaaacgac gacgaccggt agagtcatca cgacgcggag cgcggccttc acgacgttcg
    18781 ccgctaaaac gacgaccatc accacggcca ccttcacggc gttcaccgct gaagttacga
    18841 ccgccttcac gacgttcgcc accgaaacca cgaccaccgc cacgacgctc accgccagta
    18901 tgcggctgtg catcgcccag taactgcatg ttcatcggct tgttgagaat gcgagtgcgc
    18961 gtaaagtgtt gcagcacttc acccggcata cctttcggca gttcgatggt ggagtgagaa
    19021 gcaaacagct tgatgttacc aatgtaacgg ctgctgatgt cgccttcgtt agcaatcgca
    19081 ccaacgatat gacgaacttc aacaccatca tcgcggccca cttcaatgcg gtacagctgc
    19141 atatcgccaa catcacgacg ttcacgacgc ggacgatctt cacggtcacc acgcgggcca
    19201 cggtcgttac gatcgcgcgg accacggtca tcacggtcac ggaattcacg tttcggacgc
    19261 atcggcgcat ctggcggtac gatcagagta cgttcaccct gtgccatttt cagcagtgcc
    19321 gcagccagag tttcgagatc cagctcttca ccttcagcag tcggctgaat tttgctcagc
    19381 agtgcgcggt attgatccag atcgctgctt tccagctgct gctgtacttt agcggcgaat
    19441 ttttccagac ggcgtttgcc tagcagttct gcgttcggca gttctacttc cggaatagtc
    19501 agcttcatag tacgttcaat gttgcgcagc agacgacgct cgcggttctc aacgaacagc
    19561 agcgcgcggc cagcacgacc cgcacgaccg gtacgaccga tacggtgaac gtaagactca
    19621 gaatccatcg ggatatcgta gttaactacc aggctgatac gctcaacgtc caggccacgg
    19681 gctgcaacgt cggtcgcaat caggatgtcc agacgaccat ctttcaggcg ttccagtgtc
    19741 tgttcacgaa gcgcctggtt catgtcaccg ttcagcgcgg cgctgttgta gccgttacgc
    19801 tcaagagctt cagccacttc cagagtcgcg tttttggtac gaacgaagat aatcgccgca
    19861 tcaaaatctt ccgcttccag gaaacgtacc agtgcttcgt ttttgcgcat accccagaca
    19921 gtccagtagc tctggctgat gtcaggacgg gtagtcacgc tggactgaat gcgcacttcc
    19981 tgcggctctt tcataaagcg gcgggtaatg cgacgaatcg cttccggcat ggttgcagag
    20041 aacagagcgg tctgatgacc ttccgggatc tgcgccataa tggtttcaac gtcttcgatg
    20101 aagcccatgc gcagcatttc gtcagcttca tccagaacca gaccgctcag tttagagagg
    20161 tccagagtgc cacgtttcag gtggtccagc agacggcccg gagtaccgac aacgatctgc
    20221 ggcccctgac gcagggcgcg taattgcacg tcataacgct ggccgccgta cagagcaacc
    20281 acatttacgc cgcgcatgtg tttagagaaa tccgtcattg cttcagcaac ctgtaccgcc
    20341 agttcgcggg tcggtgccag caccagaatc tgtggtgctt tcagctcagg atcaagattc
    20401 tgcagcagag gtaaagagaa tgctgcagtt tttccgctcc ccgtctgggc catacccaga
    20461 acgtcgcggc cattcagcag atgtggaata cactctgcct gaattggaga tggtttttcg
    20521 taacccagat cgttaagggc ttcaaggata ggagccttca ggcccagatc tgcaaaagtg
    20581 gtttcgaatt cagccatgta gtacgtgtgc ctcaaaatta atggcggcca gtctacataa
    20641 ctcatcatga aattgatcag caattttcat tgaaaagtgt gaaccggctc aaagtaggtg
    20701 tattaacgaa caacaacgcc ctcacccgtt aaggtgatgg caatcaaaaa agattacggg
    20761 ctgatgtgta cgtcagctat tgctggtccg attctgccag gtcatcttgg tcctggccca
    20821 ggagcgataa ttccaacaat gcgtatcggt gctcaacaaa gttatgaacg ttgttggcaa
    20881 ccgccagttt gaacagtgcc gtggcgctgt ccaaatcccc cagacttagg tagtacttac
    20941 ctaaatagaa gttggtttca ctgagatgct cagcgagcga ggtgttatcc gttgcgtccg
    21001 ccttgagcct ttccattaac gtttgttcgc taatgttgcc caggtagaac tcgacaatgt
    21061 tccatcccca ctgttcctta tccgattttt cgaagtgctg tttcaacact tctttagcct
    21121 gcttctcatc gagcttctgc tcggcgagat aaagccacag actacggaaa ggatcattgg
    21181 gatcgtcttg ataaaacgcc agcagatcat cttgcgctaa cttgtcacga ccgccgtaat
    21241 ataatgcgat cccgcgattc aagtgcgcgt agttgaaagt tggatcaagc tcaagtacag
    21301 aatcaaacgc ttcataggca gcatcaaaat tgcctgcctg cgttaaatat atgcctaagt
    21361 aattgaatac ttcaggcata tccggtcgga ttgccagcgc ttgcgaaaaa tcgttacgcg
    21421 ctaatgccct cagaccgaga ctatcataca acactccgcg ctcatataaa agctgtgcgc
    21481 gttcgtcatc ggttaaagcc cgactggcaa ggatttgttc catacgtgcc agaatcactt
    21541 cctgctgtaa agtcggttgc aatggtaccg cgaggacttc acttttacgc caggaagtat
    21601 tactgcatcc tgcaagcgta agtgctgtcg caacgaaaca ccagcgcaaa aaaggcttca
    21661 tttcccactc ccgaagacca cggttgaatg aacgtcctgt tcccggttgc taacaaggcg
    21721 tcctgcccgg ttaaaagccc cccgccgcag cggagggcaa atggcaacct tactcgccct
    21781 gttcagcagc cggagcttcc ggtgctgcag caggttgaga ctgctcagtc gcttctttaa
    21841 tgctcagacg gatacggccc tggcgatcaa cttccagaac tttcaccggt acttcctgac
    21901 ccatctgcag gtaatcggtc actttctcaa cgcgtttgtc agcgatttga gagatgtgta
    21961 ccagaccttc tttaccgccg ccgatggcaa caaatgcgcc gaagtcaacg atacgggtca
    22021 ctttaccagt gtagacgcgg cccacttcga tttctgcagt gatctcttcg atacgacgaa
    22081 tagcgtgttt cgctttctcg ccgtcggtcg ctgcgatctt cacagtaccg tcatcttcga
    22141 tttcgatggt ggtgccggtt tcttcggtca gggcacggat aacagagccg cctttaccga
    22201 taacatcttt gatcttgtcc gggttgatct tgatggtatg gatacgcggt gcgaactcag
    22261 agatatcgcc acgcggcgcg ttgatagcct gttccattac gcccaggata tgcagacgcg
    22321 cacctttagc ctggttcagc gcaacctgca tgatctcttt ggtgatacct tcaattttga
    22381 tatccatctg caatgcagag ataccctcgc gggaacccgc aactttgaag tccatatcgc
    22441 ccaggtgatc ttcgtcgccc aaaatgtcag acagtacaac gtagttgtcg ccttctttca
    22501 ccagacccat tgcgataccc gcaacggcag ctttgatcgg cacacctgcg tccatcagcg
    22561 ccagagacgc gccgcacacg gaagccatag aagaggaacc gttggattca gtgatttcag
    22621 acacaacacg tacggtgtac gggaatttgt ccatatccgg catgactgcc agcacgccgc
    22681 gcttcgccag acgaccgtga ccaatttcac gacgcttcgg agaaccgacc atgccggttt
    22741 cgcctacgga gtacggaggg aagttgtagt ggaacaggaa ggtatcggta tgttcgccca
    22801 tcagttcatc aagaacctgc gcgtcacgag cagtacccag cgttgcggta accagcgcct
    22861 gcgtttcacc acgggtgaac agcgcagaac cgtgagtacg cggcagcacg ccagtacgca
    22921 catccagacc acggatcata tctttttcac gaccgtcgat acgcggttcg cctgccagta
    22981 cgcggctacg aacaacgttt ttctcgatag cgtgcagaat ttcacccagt tcgttttcgt
    23041 ccagggtttc gtcttcagca agcagcgtcg cgatggtttc agatttgatg acatcaacct
    23101 gcgcataacg ctcttgtttg tcggtgatgc ggtaagcatc gctcaggcga gcttcagcca
    23161 gtgcagcaac gcgcgcgttc agcgcttcgt ttaccggctc cggctgccag tcccaacgtg
    23221 gtttaccggc ttctttcacc agttcattga tgttctgaat aacaacctgc tgttgttcat
    23281 gaccgaacac tactgcgccc agcatctggt cttcgctcag cagttcagct tcagattcaa
    23341 ccatcagaac agcggcttca gtaccggcaa caaccagatc cagtttgctc tctttcagct
    23401 cgtcctgagt cgggttcagt acgtactggt cattgatgta acctacgcga gcagcaccaa
    23461 tcggaccatt gaacggaata ccagacagag acagtgctgc ggaagcacca atcatcgcga
    23521 cgatatccgg gttaacttgc gggttaacag aaaccacggt ggcgataacc tgaacttcgt
    23581 tgacgaagcc ttccgggaac agcgggcgaa tcgggcggtc aatcagacgc gcgatcaggg
    23641 tttcgccttc gcttggacga ccttcacgac ggaagaagct acccgggata cgaccagcag
    23701 cgtaggtacg ctcctgatag ttaacggtca gtgggaagaa gtcctgacct ggtttggctt
    23761 ttttctggcc aacaacggta acaaataccg cggtgtcatc catgctaacc ataacagcgg
    23821 cagtagcctg acgagccatc atgccggttt ccagagtcac ggtgtgttgg ccgtactgga
    23881 atttacgaac gatcggatta agcaatgtaa tatcctttct ctttcttaga cagtacctta
    23941 cggcactggt gttaataccc gatcttctgc gcatcctcgc gactaatgac aaccctaacc
    24001 cagctctatg tgggtaaagc ctctcattag ccgcgcgaac ctctgcaacg gaagatcatt
    24061 catagcaaca atacattagt ttccagtgaa ttgctgccgt cagcttgaaa aaaggggcca
    24121 ctcaggcccc cttttctgaa actcgcaaga attagcgacg cagacccagg cgctcgatga
    24181 gctgggtgta acgtgctacg tctttacgtt tcaggtagtc gagcagttta cgacgctgag
    24241 aaaccatgcg cagcagacca cgacggctgt ggtgatcttt tttgtgctct gcaaagtggc
    24301 cctgcaggtg gttgatctgt gcagtcagca gtgctacctg aacttcggta gaaccggtgt
    24361 cgtttgcgtc acgaccaaac tcagaaacga ttttagctgt tgcttcagta cttagagaca
    24421 ttttaaaact ccaaagtata tagaatgaaa ggacgccgat ctctaattca gcgatcccag
    24481 tgtacgttac gcaaagtgtt aaacaattta cgcgacgtta agcggcagta ttctactcgt
    24541 agcgacctgt tatcgcaaga cggttaacat tacgccgggt attcaaccac caggcgacga
    24601 ggcgcgacgc ggccttcatc gtcaatttcg cccataccga taaacttgcc gttctcacct
    24661 tctgttacgc gaaccagtcc ttccagcggc gcaccggatg tacgaactgg gttaccattt
    24721 ttgaagtaaa cagaagacgt taacggaaga ttcaccaccg ggtagtccga agctggactg
    24781 tccattggca tcagtaatgg atcaagtaac tccgcagccg gaatatcctg ctgttcggct
    24841 tgttcgacca gttcacgcag atgttccagg gtcaccatcc gttcaaccgg atatttactt
    24901 accgccagac gacgcaggta aataacatgc gcgccacaac cgagtttttc acccaggtca
    24961 tcaatgatgg tgcggatata agtgcctttt gagcagtgaa tttccagctc cagctcattg
    25021 ccttcgtggc gaataaacag caattcataa acggtaatcg ggcgcgcttc acgcggtact
    25081 tcaatgccct gacgcgcata ttcgtacagt tttttaccct gatatttcag tgctgaatac
    25141 atcgaaggga tctgttcgat atcgccacgg aaagtatcca gcgccgccgc cagttgctct
    25201 gcgctaaagg ttaccggacg ttcttcaacg atttgtccgt cggcatcaga agtatcggta
    25261 cgctgtccaa gacgcgcaat gacccgatag cgtttgtcgg agtccagcag atactgggaa
    25321 aacttcgtcg cttccccgag gcaaattggc aacatgccag ttgccagcgg gtccagcgca
    25381 ccggtatgcc cggcacggtt ggcgttataa atccgtttca ctttttgcag cgcatcattg
    25441 ctggacatac cctgcggttt atccagcaac aaaacgccgt taatgtcgcg accgcgacga
    25501 cgaggacgac tcattagtcc tccttgctgt cgtccgggtt aacacgacgt tcttcgtcat
    25561 gtttgaccac gctggtcacc aggtttgaca tgcgcatccc ttcaaccaga gagttgtcgt
    25621 agaagaaggt cagttccggc acgatacgca ggcgcatcgc tttccccagc aggctgcgga
    25681 tgaaaccaga agcttcttgc aacgctttga tgcccgcttt aaccgcgtct tcatctttgt
    25741 cgttgaggaa cgtcacatat actttggcat acgccaggtc gcgagacatt tcgacaccgg
    25801 aaacggtggt catcatgccc aggcgaggat ctttaatttc acgctgcagg atgagagcga
    25861 tctctttttg catttcctgc gctacgcgct gcgggcgacc aaattctttc gccataataa
    25921 attctcctga caaaaaaggt gctgttagcc cctttttaaa attaatttca ggtggaaggg
    25981 ctgttcacgt tggcctgata agacgcgcca gcgtcacatc aggcaatcca tgccggatgc
    26041 ggcataaacg ccttatcccg catggaaccc taaaaacctt aagcgatggt acgttggatc
    26101 tcgatgattt cgaatacttc gatcacatcg ccagtgcgga cgtcgttgta gttcttaacg
    26161 ccgataccac attccatacc gttacggact tcgttaacgt catctttgaa gcggcgcagg
    26221 gactccagct cgccttcgta gataaccacg ttgtcacgca gaacgcggat cgggttgtga
    26281 cgtttaacca caccttcggt aaccatacag cctgcgatgg caccaaattt cggtgatttg
    26341 aatacgtcac gaacttccgc cagaccgata atctgttgtt tcagttccgg agacagcata
    26401 ccgctcattg ccgctttcac ttcgtcgatc aggttataga tgacggagta gtaacgcaga
    26461 tccaggcttt ccgcttcaat cactttacgt gcagaggcat cagcacgtac gttaaagcca
    26521 accaggatgg cgttggacgc cgcagccagg gtggcgtcgg tttcggtgat accacctacg
    26581 ccagaaccga tgatcttcac tttaacttcg tcagtagaca gtttcagcaa ggagtcggag
    26641 atcgcttcca cagaaccctg tacgtctgcc ttcaggacaa tgttcacttc gtgaacttcg
    26701 ccttcggtca tgttggcgaa catgttctcg agtttagatt tctgctgacg cgccagttta
    26761 acttcgcgga atttaccctg acgatacagt gcaacttcac gcgctttctt ctcgtcacgt
    26821 acaacggtaa cttcatcacc cgcagccggt acgccggaca ggccgaggat ttccaccgga
    26881 atggacggac ccgcttccag cacttcctga cccagttcgt tacgcatcgc acgaacacga
    26941 ccgtattcga agccacacag aacgatatcg cccttgtgca gagtaccttc acgtaccaga
    27001 acggtagcaa ccggaccacg acctttatcg aggaaggatt cgataaccgc accgctcgcc
    27061 atacctttac gtaccgcttt cagctccaga acttccgcct gcagcaggat agcgtccagc
    27121 agttcatcga taccggtacc cgctttcgca gatacgtgta cgaactggct ttcaccgccc
    27181 cactcttccg gcaggatgcc gtactgggag agttcgttct taacgcgatc cggatcagct
    27241 tctggtttat cgatcttgtt cactgcaacc accaccggta cctgcgccgc tttcgcgtgc
    27301 tggattgctt cgatggtctg cggcatcaca ccgtcgtcgg cagcaacaac caggactacg
    27361 atgtccgttg cctgcgcacc acgagcacgc attgaagtaa acgcggcgtg ccccggggtg
    27421 tccaggaagg tgatcatgcc gttttcagtt tcaacgtggt atgcaccaat gtgctgggta
    27481 atgccgcccg cttcgccaga ggccactttc gttgaacgaa tgtagtccag cagagaggtt
    27541 ttaccgtggt caacgtgacc catgatggtc acaaccggcg cgcgcggttc agccgcagca
    27601 cccgtgtcac ggtcgctcat taccgcctct tccagctcgt tttcacgacg caggataact
    27661 ttatggccca tctcttcagc aaccagctgt gcggtttcct gatcgataac ctggttgatg
    27721 gttgccattg cgcccagttt catcatcgct ttgatgacct gagagccttt aaccgccatc
    27781 ttgttcgcca gttcgccaac ggtgatagtt tcgccgatca caacgtcacg gttaacggcc
    27841 tgagcaggct tctggaagcc ttgctgcagc gaagaacctt tacgttttcc gcctttaccg
    27901 ccacgtactg ctgcgcgtgc ttcttcacga tcagcttttg attcagcgtg tttgttgcct
    27961 ttcttcggac gcgctgcttt cgcgttacga ccacggccac ggccgccttc gacttcacga
    28021 tcgctttcgt cttctgcctg gcgagcatgt tgagaagtag tgacgtgata atcgctggaa
    28081 tcttcagtcg gttccgcgtt atcagtccat ttgttttctt ccgccatacg acgtgcttct
    28141 tcagcaacgc gacgtgcttc ttcttcgagt ttacgacgcg cttcttcttc agctttacgc
    28201 ttgagctctg cagcttcctg ctcacggcgg gctttttcag cctgggcgtt tttagtcata
    28261 tcgtcttgtt gattgctcac tttgtctttt tccgcagctt cacgtttcgc ttgttcagca
    28321 gcttcacgct tagcttgttc tgcggcctca cgttcagctt tttgttgcgc ctcgcgttta
    28381 gccgattctt ctgcctcacg acgggcttgc tcttccgctt cacgctgcgc ttgctcttcc
    28441 gctgcaaggc gttcagcctc ttgcggatcg cgtttcacaa aggtgcgttt cttgcggact
    28501 tcgatttgta ccgatttgct ttttccaccg gtaccaggaa tgttaagggt gctgcgtgtt
    28561 ttacgttgca gcgtcaattt gtccgggcct gaatttttct gattcaggtg gtcaatcaaa
    28621 gtctgtttct cttgtgcaga cacagagtcg tcagcagact tccggatacc tgcatcagca
    28681 aattgctgta ccaggcgttc cacggaggtc tgtcgctctg cggccagcgt tttaatcgtt
    28741 acatctgtca tgctgttcct tcctgctaca gtttattacg cttcgtcacc gaaccagcaa
    28801 atattacggg cagccataat cagtgctccg gctttttcat cggtcaaccc ttcgatatca
    28861 gccagatcat caatgccctg ttcggcgaga tcttccagcg tacaaacgcc acgggcggcc
    28921 agtttgaatg ccaaatcacg atctacccct tcaaggttca gcagatcgtc agccggtttg
    28981 ttatcaccga ggctttcttc ctgggcctgt gcaatggtgg ccagtgcatt tttagcacgc
    29041 tcgcgcagtg cttcaacggt cggctcatca aggccttcga tttccaacag ctctttcatc
    29101 ggcacatagg ccagttcttc cagcgtcgag aaaccttctt ctaccagaac agtcgcgaag
    29161 tcttcgtcga tgtcgagata tttggtgaag gtgtcgatcg ctgcgtgcgc ttccgcctga
    29221 tgcttagcct gcaggtcgtc aacggtcatt acgttaagtt cccaaccgct cagctgcgaa
    29281 gccagacgca cgttctgacc gttacggcca atcgcctgcg ccaggttacc ggcttcaacg
    29341 gcgatatcca tggtgtgttt atcttcatcc accacgatag aagcaacgtc tgccggtgcc
    29401 attgcgttaa tcacaaactg cgccgggtta tcatcccaca ggacgatatc gatacgctcg
    29461 ccgcccagtt cagtagaaac cgcctgaaca cgcgcgccac gcatacctac acaggcacct
    29521 accggatcga tacgtttatc gttggttttc accgcgattt tcgcacgaga acccggatcg
    29581 cgagccgctg ctttaatttc aatcacttct tcgccgattt ctggcacttc aatacggaac
    29641 agttcgatca gcatttccgg cttggaacga gtgacgaaca gttgcgcgcc acgcgcttcc
    29701 gggcgaacgg aatagagcac gccacgaacg cggtcgccag ggcggaagtt ttcacgcggc
    29761 agcatatctt cgcgcaggat cacggcttca gcattgttgc ccagatccag agagatgttg
    29821 tcgcggttta cttttttcac cacgccggtg atgatttcac cttcgtgttc acggaactga
    29881 tcaaccacca tcgcacgttc ggcttcacgc actttctgca cgataacctg ttttgccgtc
    29941 tgggtagtga tacggtcaaa ggtaacagac tcaatctgat cttcaacgta atcgcccagg
    30001 ttcaggcttt catcttcata acgtgcggct tcaagggtga tttccttggt cggctgggtg
    30061 acttcatcaa caactaacca gcgacggaag gtgtcaaaat caccgctttt gcgatcgatc
    30121 tgtacgcgga cgtcgatctc ttgttcatat tttttctttg ttgctgtcgc cagcgcgctt
    30181 tccaatgctt cgaaaatctt ctcgcgaggt agcgcctttt cattggatac ggcttcaact
    30241 acagccaaaa tttctttgtt catcgcgggc ttttcacctc atccagacta ttaaaagtgg
    30301 ggaaccaggt tcgccttctg gatattactc agcgcgaaca cttcatcttt accttcgacg
    30361 gtaactgtga tcatttcacc gtctaccgct ttgataacgc cctgccattt acgacggttt
    30421 tgtaccgcca tacggagaac cagagtcacc tcttctccga caaaacgggc gtagtgttca
    30481 gccgtgaaca gtgggcgatc gagacccggt gaggagactt ccaggttata agcaacggtg
    30541 atgggatctt caacatccag cacagcactt acctggtggc tcacatcagc acaatcatca
    30601 acattgatgc catcttcact atcaatatag atgcgcagtg tggatgtgcg accgcgaata
    30661 aattcgatgc caaccagtcc aaaacccagg gcctcaactg gcgcagtaat catctctgtt
    30721 aatttttgct ctaatgtgga caagcccacc cccaagacat aaaaaaaggg cctaaagccc
    30781 agttattctg tagtcagata acaaaaaacc ccgataaatc ggggctttat ataactgaac
    30841 cctataaccg caactgcggt ctggagcact ttccagaagg attttttcaa atcccactac
    30901 gaaggccgaa gtcttcacag tatatttgaa aaaggactct aagggaaagt ggttgcgggg
    30961 gccggatttg aaccgacgac cttcgggtta tgagcccgac gagctaccag gctgctccac
    31021 cccgcgcctg aaacgtggca aattctactc gttttgggta aaagatgcaa atactgctgg
    31081 gatttggtgt accgagacgg gacgtaaaat ctgcaggcat tatagtgatc catgccacat
    31141 tttgtcaacg tttattgcta ttcacgtgaa tgaatatcca gttcactttc attttttgaa
    31201 tacttttgcc ttctcctgct ctcccttaag cgcattattt tacaaaaaac acactaaact
    31261 cttcctgtct ccgataaaag atgattaaat gaaaactcat ttattttgca taaaaattca
    31321 gtgagagcgg aaatccaggc tcatcatcag ttaattaagc agggtgttat tttatgacga
    31381 cgattctcaa gcatctcccg gtaggtcaac gtattggtat cgctttttcc ggcggtctgg
    31441 acaccagtgc cgcactgctg tggatgcgac aaaagggagc ggttccttat gcatatactg
    31501 caaacctggg ccagccagac gaagaggatt atgatgcgat ccctcgtcgt gccatggaat
    31561 acggcgcgga gaacgcacgt ctgatcgact gccgcaaaca actggtggcc gaaggtattg
    31621 ccgctattca gtgtggcgca tttcataaca ccaccggcgg cctgacctat ttcaacacga
    31681 cgccgctggg ccgcgccgtg actggtacca tgctggttgc tgcgatgaaa gaagatggcg
    31741 tgaatatctg gggtgacggt agcacctata aaggaaacga tatcgaacgt ttctatcgtt
    31801 atggtctgct gaccaatgct gaactgcaga tttacaaacc gtggcttgat actgacttta
    31861 ttgatgaact gggcggccgt catgagatgt ctgaatttat gattgcctgc ggtttcgact
    31921 acaaaatgtc tgtcgaaaaa gcctactcca cagactccaa catgcttggt gcaacgcacg
    31981 aagcgaagga tctggaatac ctcaactcca gcgtcaaaat cgtcaaccca attatgggcg
    32041 tgaaattctg ggatgagagc gtgaagatcc cggcagaaga agtcacagta cgctttgagc
    32101 aaggtcatcc ggtagcgctg aacggtaaaa cctttagcga cgacgtagaa atgatgctgg
    32161 aagctaaccg catcggcggt cgtcacggcc tgggcatgag cgaccagatt gaaaaccgta
    32221 tcatcgaagc gaaaagccgt ggtatttacg aggctccggg gatggcactg ctacatatcg
    32281 cttatgaacg tctgttgacc ggtattcaca acgaagacac cattgagcag tatcatgcac
    32341 atggtcgtca gttgggccgt ctgctgtacc aggggcgttg gtttgattcc caggcgctga
    32401 tgctgcgtga ctctctgcaa cgctgggttg ccagccagat cactggtgaa gttaccctgg
    32461 agctgcgccg tgggaacgat tattcaatcc tgaataccgt ctcagagaac ctgacctaca
    32521 agccagagcg tctgacgatg gaaaaaggcg actcggtgtt ctcgccagat gatcgtattg
    32581 gtcaattgac catgcgtaat ctggatatca ctgatacccg agagaaactt tttggttatg
    32641 ccaaaactgg cctgctctcc tcctctgcca cttcaggcgt gccgcaggtg gagaatctgg
    32701 aaaacaaagt taagtaatat catagctttc gtgctaaagg ccgcaaatgc ggcctttttt
    32761 tacggctaag agaatgtgac tgcgtttagg ggccaccatg tccgaattcg gaattaaagt
    32821 aaaagttaaa tgaaataaag taaatttcag cgcattctat ttttacttta tgtttttatg
    32881 ctcagtaaaa ggaatgaccg atgaatggtg aatattttgt acgcctcgca ttacatactc
    32941 tgaaatgcac tcaaaaagat ttagcaagcc acttaggtgt ttcctcaaca caaattagca
    33001 agtggaaaaa aggggaacac atgtccgctg atatggaaaa aaagttcaga aatattacac
    33061 aaataggaga ttattctcct caactagttg aatggacggg ctcagtcgcg aatgctgaga
    33121 aatgggatcg acttatccat tttcttgctc gacgagctat ggacgatgct gaaacgggtt
    33181 atataacaca acttcttact gacgaagatg gttttttggt tgaagaaaca attgatgtat
    33241 taaagcgaat aggttttcct acacctcttt cctttcctga aggacttaat attgacgacg
    33301 ataatgctga tgaagaagag gctttttggg agatattaga gagcaatgct cattgttctg
    33361 taattaatga tatttatcat gctctgaatg atgtttatgg tttctacata gcatatgttg
    33421 atgagttgat tcaggacgac gatttagatg tctattcaag cgaagccatc aatattcaga
    33481 gttcattgat atcacttgct gcatgcaaaa ttgaaattga tactcctgtt gcgtcaaact
    33541 ttaaagaatt tcgttataga gttaaaaaag actacgaaaa ttggttgaat caactcaaga
    33601 tgatggcatt cagggcggga attccgttaa gagccgaact tctggaaatg gtttataaca
    33661 ccgctgacca acttagcgtt gcagcagaag ccgaacgttt cgatttcaat aaatctcgca
    33721 ttcatccaga tatatacatg aacgagatcc ttactggcat gagaataatt caccaagtgc
    33781 ttccggtaat aatgcaaaag ttagaaatta cagatttcaa actagatgaa acagatttat
    33841 gcctgggtaa atagttatca aattaagaat acttagcctg gcttataaaa cgaaaaaaga
    33901 cgcttttcag cgtctctttt ctggaatttg gtaccgagga cgggacttga acccgtaagc
    33961 cctattgggc actaccacct caaggtagcg tgtctaccaa ttccaccacc tcggcacgga
    34021 tactactttt tagttcggga tatcgctggt cggcttagcc ggagcagctg gctgagtttg
    34081 ttcggttttc gccggtgcac tcagattttc ccattcgcta cctttattgg ttttgttgct
    34141 attgatgtta cccagcacca gactgatgat gaagaataac gttgccagca gcgccgtcat
    34201 gcgggtcatg aagttaccag aaccacttga accaaacagc gtagcggaag cgcctgctcc
    34261 gaaggaggct cccatatcag cgcctttacc ttgctgcagc atgatcagac caacaaggcc
    34321 aattgccaca ataaggaaaa ctaccaaaag agcttcgtac ataatcaacc tgttccttgc
    34381 ggagttgccg cgtaccaatg cttcaaccaa taaagcggga gtttttaatc tttcccactg
    34441 aagcgggtgt gaatactaac caaagcgaat gaccttcgca agggcaattt tatcgcattg
    34501 tatcaactgc ggaaaaaaac agcaaaaccg atgtgttcgc tgagaaaaaa ggcgacaatc
    34561 gccgcctttt tagccagtca tttatcgttt taaacggctt ttactgcatc ggcgatgcgg
    34621 tgtgcaaatt cagtcacctg cgcttcgtct tcaccttcta ccatcacgcg aattaacggt
    34681 tcggtgccgg atttacgcag caacacgcgt ccacggttgc ccagcgcagc ttcaacctct
    34741 gcggtcacgg ctttaactga ctcatgctca agcggatcac cgctacctgc ggtgtaacgt
    34801 acgttaacca gaatctgcgg gaacattttc atgccgctgc aaaggtcgtg caggctcata
    34861 tggttacgtg ccatcgccgc cagcacctgc aagccagcaa cgatgccgtc accggtagtg
    34921 gttttatcca gcaggatcac atgaccggaa ttctctgcac cgatacgcca gcctttctcc
    34981 tgcatttttt ccagtacgta gcggtcaccc actttcgcgc gcgcaaatgg aattcctaac
    35041 tgtttcagcg ccagttcaag ccccatgttg ctcatcaatg tacccacagc gccaccacgc
    35101 agctggccct gacgaagacc ttcacgcgcg atgatataca tgatctgatc gccatcgact
    35161 ttattgcctt catggtcaac cataatcacg cgatcgccgt cgccgtcgaa ggcaataccg
    35221 agatccgctt tttcagccag cacacgagcc tggagcgcgc gaacgtcggt agccccaact
    35281 tcggcattga tgtttacacc gtttggctca caaccgatag cgataacgtt cgcccccagt
    35341 tcgcgcagca cgttcggcgc gatgtgataa gtcgcaccgt ttgcacaatc caccacaatc
    35401 ttcagttcac tgaggctaag ttcgttcggg aatgtggctt tgcaaaactc gatatagcga
    35461 cccgcggcat caacgatacg gctggcttta cccagttctg ccgaatcaac gcagctgatc
    35521 tccttttcca tttccgcttc gatggcctct tctaccgcat ccggcagttt ggtgccgtcg
    35581 atagagaaga atttaatccc attatcgtag aacgggttat gcgatgcaga tatcacaatt
    35641 ccggcctctg cgcggaaggt acgcgtcaga taagccacgg ccggtgttgg catcgggcca
    35701 gtgaagagtg cggaaaggcc cgctgccgcc agacccgctt ccagtgctga ctccagcata
    35761 tagccagaaa tacgcgtgtc tttaccaata ataatcttac gggagccgtg gcgcgccagc
    35821 actttacccg cggcccaacc cagcttaagc acaaaatcag gtgtgatcgg cgcatcccct
    35881 acacgaccac gaatcccatc ggtaccgaaa tatttacgat tactcatagc gtttgttttc
    35941 ctttgcagac agagtggctt ccaccacccg catcgcttct acggtttctt tgacgtcatg
    36001 aacacgaatg atgtgcgcgc cttgcattgc ggcaatgacc gcacaggcca gactaccgct
    36061 caggcgctcg gacggcccca cgttcagcag ctgcccaatc atcgattttc gtgacatacc
    36121 caccaacagc ggcaggttga aatggtgaaa ttcagccagg cgcgccagta atgaatagtt
    36181 atgggagaga tttttaccga aaccgaatcc ggggtcgagc aacaatttct cttttgcgat
    36241 acccgcctgc tcgcaacgtg ctatttgctc aataaagtag cgattcactt ctgcaaagac
    36301 atcgtcatac ttcggagctt cctgcatggt ttttggattt ccctgcatat gcatcagaca
    36361 aaccggtaaa ccggtttctg cagccgcctc cagagcgcca ggttcggaaa gggagcggat
    36421 atcattaata atgtgagcgc caactttcgc tgactcacgg atgacttctg gtttggatgt
    36481 atctaccgag atccagactt cgaagcgttg agcaattgcc tcaaccacag gaataacacg
    36541 ttgcaactct tcttcaacgc taacttccgc cgcccctggg cgcgtggact cgccaccaac
    36601 gtcaatgatc gtcgcgccag cgttgatcat cagattcgca tgtttcaccg catctatcag
    36661 cgagttatgc atgccaccat ccgaaaagga atcaggcgtg acgttgagga tccccattac
    36721 gtgaggatgg ctaaggtcca gtgaagtgcc ctgggcaaag agtttcatgg tgttatccct
    36781 ggtatgaatt gataagaaaa aaccccggag cacgccccgg ggttttcggt acaaatacag
    36841 tcatctgatg cgggaactta cttgtcgcct aactgctctg acatggtgtt acccgggttc
    36901 ggcgtacgcg gttcatcaac cggacgagga gcctttggac taccattgtc gccagaattg
    36961 ttagaagcgc ctggttcttc ccagcccgct ggcggacgta catcgcgacg tgccatcagg
    37021 tcatcaatct gcggtgcgtc gatagtctca tatttcatga gagcatcttt catcgcatgc
    37081 agaatatcca tattgtcggt cagaagctga cgcgcacgat tatagttacg ctcaatcagt
    37141 gctttcactt cctggtcgat gatacgtgca gtttcatcgg acatatgttt cgctttcgct
    37201 acgctacggc cgaggaacac ttcaccttct tcttccgcgt acagcagtgg acccaatttc
    37261 tcagagaagc cccactgagt caccatgtta cgcgccaggt tggtcgcaac tttaatatcg
    37321 ttggacgcac cggtagaaac atgttccggc ccgtagatga tctcttctgc cagacgacca
    37381 ccgtacagcg tagaaatctg gctttccagt ttctgacggc tggcgctgat tgcgtcgccc
    37441 tcaggcaaga agaaagtcac acccagcgca cgaccgcgtg ggataatcgt cactttgtgc
    37501 accggatcgt gttccggcac caggcgaccg ataatcgcat gacccgcttc gtggtaagcc
    37561 gtcgattctt tctgcgcttc cgtcatcacc atggagcgac gttccgcacc catcatgatt
    37621 ttatctttcg ctttctcgaa ctcaaccatc gacacaacgc gtttgttgcc acgagcagcg
    37681 aacagtgccg cttcgtttac caggttcgcc aggtcagcac cggagaaacc aggagtacca
    37741 cgggcaatga ttgccgcgtc gatatcgggt gccaatggta cgcgacgcat gtgaactttc
    37801 aggatctgct cacgaccgcg aacatctggc aagccaacca caacctgacg gtcgaaacgg
    37861 ccaggacgca gcagggccgg gtcaagaacg tccggacggt tagtcgcggc gataacgatg
    37921 ataccttcgt taccttcgaa gccatccatc tcaaccagca tctggttcag agtctgttca
    37981 cgttcatcgt gaccaccgcc cagaccagcg ccacgctggc ggcctacggc gtcgatttca
    38041 tcgataaaga tgatgcacgg tgccgctttc ttcgcctgtt cgaacatgtc acgaacacgg
    38101 gatgcaccca caccgacgaa catttctacg aagtcagaac cggagatagt aaagaacgga
    38161 actttcgctt cgcctgcaat cgctttcgcc agcaacgttt taccggtacc cggaggaccg
    38221 accatcagga cgcctttcgg gatcttaccg ccgagtttct ggaagcggct cggctcgcgc
    38281 agatactcta ccagttcagc aacttcttct tttgcttcgt cgcagcccga aacgtcagca
    38341 aaggtcgttt tgatctgatc ttccgtcagc atgcgcgctt tgctcttacc aaacgacatg
    38401 gcacctttgc caccgccgcc ctgcatttga cgcatgaaga agatccagac accaatcagc
    38461 aacagcatcg ggaaccagga gatgaagata gaagccagca ggcttggttc ttcaggcggt
    38521 tcaccgacaa ccttgacgtt cttggtcaac aggttatcca gtaatttcgg atcctgaacc
    38581 ggaatgtaag tggtataacg gttactatct ttcttggtaa cgttgatttc acgtccgttg
    38641 atacgcgctt cacgaacctg gtcgttattg acctcttgta ggaaggtaga gtaatccacc
    38701 ttacggccat tagactcgct gggcccaaag ctctggaata ctgacatcag cacaacggca
    38761 atgaccagcc agagtattag gtttttcgcc atgtcactca agggattaac ctcttattac
    38821 aactgtgtta aaaacagcgt caggatactc tatatccagc atctttcaaa ctttcgtctg
    38881 aaatctcccg gttagggttt acgcccggtc gctacaatat acacttcccg cgaacgtgca
    38941 cgagaagagt ccggcttacg aactttgacc ttcgtaaaca gggagcgaat ttcccttaga
    39001 tactcatcga aaccttcgcc ctggaacacc ttcactacaa aactgccacc tggcgctaat
    39061 acatcacgac acatttctaa cgccagttcc accagataca tggcacgggg gatatccacc
    39121 gccggtgttc cgctcatgtt tggtgccata tcggacatga caacctggac tttgctgtcg
    39181 ccaacccgct ccagcagtgc tttcatcacc agttcatcac gaaaatcgcc ctgaagaaag
    39241 tccacaccaa cgataggatc cataggtaaa agatcgcaag cgatgatgcg gcctttgccg
    39301 ccaatttggg tgaccacata ttgtgaccaa ccacccggag cagcaccaag gtcgacaacc
    39361 gtcattcccg gtttaaagag tttgtcactt tgctgtattt catcaagttt aaaccaggca
    39421 cgggaacgta accccttttt ctgtgcctgt tgaacatatt tatcgctaaa gtgttcctga
    39481 agccagcggc tggagctggc agaacgcttc ttacctgtca tttaactttc ccatggggat
    39541 aactcatcgt aaccaattgc gtaaattttt acgcgcctat ttggtgatat atgggagatg
    39601 gcggtagaat gacccgtttt caatcccaac gtaagcaaaa aatacgatga atctgagtac
    39661 taaacaaaaa cagcacctga aaggtctggc acatccgctc aagccagttg ttctgcttgg
    39721 cagtaatggt ttgaccgaag gggtgctggc cgagattgaa caagcgttag agcaccatga
    39781 actcatcaag gtgaaaatcg ccaccgaaga tcgcgaaaca aaaaccttga tcgtggaagc
    39841 tatcgtgcgc gaaaccggcg cctgtaatgt acaggtcatc ggtaaaacgc tggtgcttta
    39901 tcgcccaact aaagaacgta aaatctcgct gccacgctaa gattatccta aagttacaca
    39961 catttgctgt gtaaaacgag gggttttccg caggcaggag agcaaaatgc cacgctctgt
    40021 tcgttgataa aaggccgcat agcggccttt ttcctttctt tacaatacat caacatcttg
    40081 agtattgggt aattcttaca ggtattccac cttaattact tcaaattcta cttcgccgcc
    40141 cggcgttttg atgaccacaa catcatcttc ttctttgccg atcaggccac gagcaatagg
    40201 cgagtttaca gaaatcaggt tttgtttaaa gtcagcttcg tcatcgccaa cgatgcgata
    40261 agtctgttct tcgtcagaat ccagattcag caccgttacg gtagcaccaa aaataacgcg
    40321 cccattgttg ggcattttgg tgacatcaat cacctgcgcg ttcgacagct tggcttcgat
    40381 gtctttaata cggccttcgc agaaaccctg ctgttcacga gctgcgtggt attcggcgtt
    40441 ttctttcagg tcgccatgct cacgcgcttc cgcgatagca gcaatgattt caggacggcg
    40501 cacagatttc agaaaatcca gctcttcgcg taatttttca gcgccgcgta aggtcatcgg
    40561 aatagcttgc atttgttata cctcttgaat attcctgatg gggcaagtct tcacccaccc
    40621 cggctgttcg gcctgcccgg cataactcct caccgggacc agaagcaaaa aaataccgac
    40681 ccgggtacaa gtcccaggtc agctacaatt cacattttga tagtcatttt accctgaagt
    40741 tcccgaaggg tcatcgttta ctttataggg cgttgcgccg tagtatgacg gctcgattcc
    40801 aggttgttag cgcgagatta tgcgattttc cagatttatc atcggattga ccagctgtat
    40861 agcgttcagt gttcaggccg cgaatgttga tgagtacgtt actcaactcc ccgctggtgc
    40921 caaccttgcc ctgatggtgc aaaaagtcgg cgcgtcggcc cccgctattg attaccacag
    40981 tcagcagatg gcgctgcccg ccagtacgca gaaagtgatt actgcgctgg cggcgttgat
    41041 tcaactcggc cccgattttc gtttcaccac gacgcttgaa accaaaggca atgtggaaaa
    41101 cggcgtactt aagggtgact tagtggcgcg atttggtgcc gatccgacgt taaaacgtca
    41161 ggatattcgc aatatggtcg cgactttgaa aaaatctggc gtcaaccaaa tcgatggcaa
    41221 tgtgttgata gatacctcca ttttcgccag ccacgataaa gcccccggct ggccatggaa
    41281 tgacatgaca caatgcttta gcgctccgcc tgccgctgcc atagttgacc gcaactgttt
    41341 ctccgtctcg ctctacagtg ccccaaaacc tggtgatatg gcttttatac gcgtggcatc
    41401 ttattacccc gttacgatgt tcagccaggt acgcaccctc ccccgaggtt ctgccgaagc
    41461 gcaatactgc gaactggatg tcgtaccagg cgacctgaac cgctttacgc tgacgggatg
    41521 cctgccacaa cgttctgagc cgctcccgtt ggcttttgcc gtgcaggatg gagccagcta
    41581 tgccggggca attctgaaag atgagttaaa acaggtaggt atcacctgga gcggaacact
    41641 gctgcgccag actcaggtta acgaacctgg aacggtagtt gccagtaaac agtcggcccc
    41701 gttgcacgat ctgcttaaga ttatgctgaa aaagtcggac aacatgatcg ccgatacggt
    41761 tttccgcatg ataggccatg cgcgcttcaa tgtgcctgga acatggcggg ccgggtcgga
    41821 cgccgtgcgt cagatcctgc gccagcaagc cggtgtcgat attggaaaca ccattattgc
    41881 cgatggttca gggctttcgc ggcataacct gattgccccc gccaccatga tgcaggtgtt
    41941 gcaatacatt gcccaacacg acaatgaact taactttatc tccatgctgc cgctggcggg
    42001 ctatgacggc tctttgcagt accgtgcagg tctgcatcag gcgggcgtgg atggaaaagt
    42061 ctcagcgaaa accggttcgt tgcagggggt atataacctg gcgggattca ttaccacagc
    42121 gagcgggcaa cgaatggcgt ttgtacaata tctttctggc tatgcagtag aaccggcgga
    42181 tcagcgtaat cgccgtattc cgttagtgcg ttttgaaagc cgtttgtata aagatattta
    42241 tcagaacaat tagtcaaaaa gaaaccccgg cacatggccg gggttttgct gattaacgct
    42301 tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcatcatcc cagtcctcat
    42361 catcctcttc agcaatctct tcaagctgct ggcgatgata atcatcccac atgaattcga
    42421 ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg ataaaggtca
    42481 tcacatccca gcagagatct ttcacgccca gtccactcgc cgcagagatc agataatatt
    42541 tatcttccca gcccagcgct tcagcaatcg ctttcgcttt ctcttcaact tctgccttat
    42601 ccagcagatc gatcttattg aacactaacc aacgcggttt cgccgccaga tcctggctgt
    42661 atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg ccatcaatcg
    42721 gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc aggaagcgga
    42781 tacccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg gcaacaacga
    42841 agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg gtaaacggat
    42901 aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat ttacccgcgt
    42961 ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc agctcacgct
    43021 tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac gatttgaaac
    43081 gggtattgcc cagaccgtgc cagccgccct tagcaagcag cagacgctga ccgtgtttgg
    43141 tcatatcgcc catggtttca ccagtaccct ggtcgattac acgcgtacct accggcactt
    43201 taattgtgac gtctttaccg cgcttaccgg tacagtcgcg gcttgcgcca ttctgaccgc
    43261 gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg ttctcgtcgg
    43321 cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg cctttcggaa
    43381 tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca acgaccagaa
    43441 tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct gcgcggggtt
    43501 gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca atggcggaat
    43561 acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt aacatcggtc
    43621 tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc gtaaacagtg
    43681 gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc gcttccgcca
    43741 gggcgccata tcctaccaat gtattcagtc cgcaaaaaat taaacatgcg agctgccagt
    43801 ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcaatt gtacataaag
    43861 tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc ttttgcgcca
    43921 cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc caggtgtaat
    43981 cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc aggccgctca
    44041 ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg ataaatacgc
    44101 tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg gtcggactca
    44161 ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg gtcgccactg
    44221 ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac ctcttcttca
    44281 ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac acgattgtcg
    44341 gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc cagcaaatta
    44401 ctgtggtgag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg tattccctgc
    44461 aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat cgaatgtaaa
    44521 aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa acttattcag
    44581 cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc actttaccgt
    44641 ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca gcgtggaatt
    44701 tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca ccgaaacgct
    44761 taacgcccag gcgtttagct tctgaatcgc gaccgttacg tgtggagccg ccagcctttt
    44821 tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa ttttcacatc
    44881 agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac gaaacttaac
    44941 gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta cgccgccatc
    45001 aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca cttcagcgaa
    45061 ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct gaccttcgct
    45121 tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact ccgcttccgc
    45181 gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc gcgaatatta
    45241 cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa aaacacaact
    45301 tgtacggtaa cgtttatctg tgccattttt tcagtacaat caccctatat tcctaaccat
    45361 aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc ggcttttgcg
    45421 atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt taatgcggca
    45481 atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta ttacatcgtc
    45541 agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg agctgttggc
    45601 tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca cacggcgact
    45661 ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc taccgccaac
    45721 gctgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac ccgcgcgttc
    45781 cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga agctgtaaac
    45841 gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga catcactgaa
    45901 gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc tgccgcgcag
    45961 tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca ggattatggg
    46021 cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa tgccgatggc
    46081 gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac gctgccgctg
    46141 ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac cgccatcgaa
    46201 cagggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc ttgtggatct
    46261 cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc agcgttacag
    46321 gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat cgctgttcaa
    46381 cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc cagtaaattc
    46441 cataaaaaca ctaattcagc gctataacac ctgaaaaaaa gggcgatgaa tctttatata
    46501 ttctgaatat tcacactctt tacaggaact ttttagagca ataggccatc aggagtatag
    46561 tgatgcccga cagaagaagt gttctgaatg aaagcgacta acttaaggag tgaggaaaga
    46621 tggaaagtaa tttcattgac tggcatcccg ctgacatcat tgcaggtttg cgcaagaaag
    46681 gaacgtcaat ggcggcggaa tctcgcagaa atggtttgag ttcctcaacg ctggcaaacg
    46741 cattatcgcg cccagggccg aaaggagaga tgattattgc gaaagccctg ggaactgacc
    46801 cctgggttat ctggccatca cgctaccatg atccgcagac ccatgagttt atcgacagaa
    46861 cgcagttgat gcgtagctac actaaaccga aaaaatgagt ggtctggcgg tagccccgcg
    46921 aacggggctg ccagctctca gacgattact cgcctttcac acgctcaata tttgcaccta
    46981 aagcgcgcag tttgtcttca atgcgttcgt agccacgatc gatgtgataa atacgatcaa
    47041 ccaccgtcgt cccttccgca atacagccag ccagcaccag gcttgctgat gcacgcagat
    47101 cggttgccat aacctgtgcg ccagaaagtt tttcaacacc gtgacaaata acggtattgc
    47161 tttcgatttc ggcatgcgcg cccatacggc taagctccgg cacatgcata aagcggtttt
    47221 caaagaccgt ttcggtgatg aatccggtcc cttctgccac caggttcaac agcgtgaact
    47281 gggcctgcat atcggtcggg aatgccggat gcggcgcggt acgtacgtta acagccttcg
    47341 gacgtttgcc atgcatatcc aggctaatcc agtcttcgcc gacttcgatg tccgctccag
    47401 cgtcacgcag tttcgccagc acggcgtcga gagtatctgg ctgcgcgtta cggcagataa
    47461 ttttgccgcg agaaatcgcc gccgccacca ggaaagtacc ggtttcgata cgatccggta
    47521 gcacgcgata gacaccgccg cctaaacgtt ccacaccttc gatgacgata cgatcggtgc
    47581 cctgaccgct aattttcgca cccagcgtaa tcaggaagtt cgcggtatcg acgatttccg
    47641 gttcacgcgc tgcgttttca ataatcgtgg taccttccgc cagggttgca gcacacatga
    47701 tggtcaccgt tgcgccaacg ctgactttat ccatcacgat atgtgcgcct ttcagacgac
    47761 catcgacgga agctttaacg taaccttctt ccagtttgat ggtcgcgcct aattgttcga
    47821 ggccagaaat gtgtagatca accggacgcg caccgatcgt acaaccgcca ggcagtgaaa
    47881 cttgcccctg accaaagcgc gctaccagcg gccccagtgc ccagatagaa gcacgcatgg
    47941 ttttaaccag atcgtaaggt gcgcagaata cattaacgtc gcgggcatca atatgcacag
    48001 aaccattacg ttctactttc gcacccagct ggcttagcag cttcattgat gtatcgacgt
    48061 ctttcagttt cgggacgttc tggatctcta ccggttcttc cgccagtaga gcggcaaaaa
    48121 ggataggcag agcagcattt ttagcgccgg aaattgtgac ttcgccctgg agcttcgttg
    48181 gcccctgaac acgaaattta tccatttagt ttgttctcag ttaacaattc atatccgcta
    48241 ccggcgaatc gcccatagct caaaagccgt tcagtttgcg atcgcgcgcc cactccgcag
    48301 gggtatacgc tttgatcgac acagcatgaa tgcggttatc cgcaatatat tccatcagcg
    48361 ggccatagac cgtctgctgt tttttaaccc gactcatgcc gtcaaacaac tcacccacgg
    48421 caataacctg aaagtggctg ccatcgccgg aaacgtggac ttcctggagg gagagagcgt
    48481 tcatcaacac gctctgaatt tcattatttt ccatgggatc ttcaatcatc agttaataaa
    48541 ccagcgaaac atcttagagc aaagttgcgc tggcataaat aagcaaaaag cctcgctgat
    48601 aaatcagaca aggctcgact tgcaggcagg tttgccggac aggcggttaa cgccatatcc
    48661 ggcctgaaaa aatttaatga ggcagaacat cagcaggcaa attatacaat ttcgcgaggg
    48721 tatacacttt gtcgtttacc ccctgaagcg tcacgttgtt gccctgcttt ttcgccagat
    48781 cgataagatg gagcagcagt gccagtccac ccgtatccac gcgggacaca cggctaagat
    48841 cgatgcaggt aacccccttc accgcttcct cacgcatttc ccaaagcggt agcagaacgt
    48901 cctgatccag ctctccggat aacgccagcg tgtcacccgt ctgcatccag ctcagtgact
    48961 cgctcattat tttttctctt ccagagtgat tttctgttga gaaatcgatt tcagttgcgc
    49021 agtcaggccg tcgatacctt tggtacgcag cagcgttccc cactcgtttt gtttggtggt
    49081 gatcatactg acgccttcag caatcatgtc gtaagcctgc cagttgcccg tctgggagtt
    49141 tttacgccac tggaagtcca gacgcaccgg cggacggcca ttcgggtcaa taatggtaac
    49201 gcgaataggc acaatggttt tatcgcccag cggctgttct ggcgcaatct gataggtttg
    49261 accgtgatac atcgccagcg cctgaccgta agcctgcttc aggtactcac ggaaagcggc
    49321 aaagtaggct tcacgttgag caggggtcgc actcttgtaa tactggccca gcaccagcgc
    49381 accggcgtat ttcacctgta cgtatggcag cagttcctga tcaacaatgg tacgcagata
    49441 atccgggttg gcccgaattt gcggttgctc attcttcagg cgatcgaacg ttttctgcgc
    49501 cgcctcgtcc atcagcttat aaggattggt ctggtctgcc gcggttgccg cactcagagg
    49561 tgcaatcacc agcaaagcga ccatcattaa acgtttaaac atgcgtcggt tctcctgaaa
    49621 ttatttcgtt gtacccacag gttcagtggt ttcattatta cctggcgcag cagctggcgc
    49681 atcgccacta ttcttattgt catcgccttt actaccgtaa aggaactgac caatgagatc
    49741 ttccagcacc atcgcagact tagtgtcctg aattgtatcg ccatccttca ggatagcagt
    49801 ccccagttcc gggtcttcaa aaccgacgtt taatgccaga tattgttccc ccagcaggcc
    49861 ggaagtacga atgctcagcg aactggtatc tggaatgtgg ttataacgtt gttcaatttc
    49921 cagcgttacg cgcggcagat aggttttcgg gtccagcgta atatccgcca cccgacccac
    49981 aacaacgcca ccaatactga ccggagagcg cgctttcagg ccgccaatgt tatcgaacgt
    50041 cgcataaagc gtgtaggtcg gttcagtacg tatggacgtc acgttcgccg ccttcaggca
    50101 aacaaacagc gccgccagca gtgctgctaa taaaaagata cccacccaaa tttcattttt
    50161 tttcgtttgc atgaactcaa ttcccaaaca tcaatgcggt cagcacaaaa tccagcccca
    50221 gaacagccag agacgagtgg acaacggtgc gagtggttgc ccggctaatc ccggcagacg
    50281 tcgggatggc gtcgtagccg ttaaacaacg aaatccacgt caccgtgatg gcgaacacca
    50341 cgctcttaat cagacagttg accagatcca tacgccagtc gacggcattt tgcattgccg
    50401 accagaagaa cccgctatca atgcctttcc aactgacgcc gaccagagat ccgccccaga
    50461 tccccacggc gacgaaaata accgtcaaca gtggtaatga aataacccca gcccagaaac
    50521 ggggagaaat aacccgacgc agcggatcca ccgccatcat ctccatactg gagagttgct
    50581 ctgtagcgcg catcaggccg atttctgcgg ttagcgccga accagcacgc ccggcaaaca
    50641 acaacgcggc aaccaccggc cccagttcac gcagtagcga taacgccacc agcataccca
    50701 gactggtttc cgcactataa gtggtcagaa ccagataacc ttgcagcccc aacaccattc
    50761 cgatgaacac gccagaaacc acaataatca gcatcgacag gacgccgaca ttatagagct
    50821 ggcgcaccag cagcggcgca tgtttgcgaa attccggttt gccgaccagc gcattgaata
    50881 acattaaccc ggcccgcccg aacgttctca gggttttaat ccctttatgt ccgagcgacg
    50941 ccagcgcatt taacagcatg agtggcttaa ctccctggta aaagatcagc gtgataatcg
    51001 ccggcaggat agcggaacgg aacaggcccg tcagctatcc cgtccagaaa ctgacgtacg
    51061 cgcggatcag gattcgcctg caacgcctgg gcactgccat gagcgacaat ttttttgtcc
    51121 gccaggatcc aggcgtgatc cgcaatactt aacacttccg gcacatcgtg agaaaccacc
    51181 acacaagtca cgcccagcgc gctgttcagc tcagaaatca gcttcaccag tacgcccatg
    51241 gtgatgggat cttgcccaac aaaaggttca tcaaacatga tgagatccgg ctccagcgca
    51301 atcgcacgcg ccagcgctgc acgccgcgcc atcccaccgg aaagttcaga aggcattagt
    51361 ttagccgctc cacgcagccc cacggcctcc agcttcatca tcaccgtact atgcaacaat
    51421 ggcgcgggaa gttgggtatg ttcgcgcagt ggataggcga cgttgtcaaa tacgttcata
    51481 tcagtgaaca acgccccgga ctgaaataac atgctcatcc gtttgcgcac tgtatacagg
    51541 cgcgaacgag acatcgccgg aatattctca ccatcgaaaa ggatctcacc atgatctggt
    51601 gcgatttgcc cgccaatcag acggagtagc gtcgttttac cgatgcccga tggccccatg
    51661 atcgccgtga tcttccctcg cggcacggtc agggaaatat tatcgaagat gcagcgattg
    51721 ccacgcgtaa aactgacatc gcgcatatcg actaaattcg ccacagactg ctccataatt
    51781 cacccttcgt cttgcgttga ttttctaagc atggcgctca atttaacctt gaacccaaca
    51841 tatttacaga atattacccg ccgtggttag cgaaagctgg catttgtttt actttttagc
    51901 cgcataaagt caaaattaag aatccgttac ggctttctga aaatcttcag cggaccggcg
    51961 agtatacctg aagaaaggac gttagatgct tttagctacg gcactgttaa ttgttggttt
    52021 acttttggtc gtttacagtg ccgaccgcct ggtttttgcc gcgtctattc tttgccgaac
    52081 ctttggcatc ccgccgctga tcatcggcat gacggtggtc agtattggta catcgttacc
    52141 agaaatcatc gtctcgcttg ccgcgtctct gcacgaacaa cgcgatttag ccgttggtac
    52201 agccctcggc tcaaacatta tcaatatttt gctgatcctc ggtctggctg cgctggttcg
    52261 tccttttacc gtccattccg atgttctgcg tcgtgaatta cccttaatgt tgttggtcag
    52321 cgtagtggcc ggttccgtac tctatgacgg acaacttagt cgcagcgatg gtatctttct
    52381 cctctttctg gctgtgctat ggctgctgtt cattgttaaa cttgcacgtc aggctgaacg
    52441 tcaggggact gacagcctga ccagagagca gcttgcagag ctgccgcgtg acggcggatt
    52501 gcccgtcgcg tttttatggc tcggcattgc gcttatcatc atgccagtgg ccacgcggat
    52561 ggtggttgat aacgccacgg tgctggcgaa ttactttgcc atcagcgagt tgacgatggg
    52621 tctgacggca attgctatcg gaaccagcct gccggaactg gcaaccgcaa tagcgggggt
    52681 tcgcaaaggt gaaaacgaca ttgctgtcgg aaatatcatt ggcgcaaaca tttttaatat
    52741 tgtcatcgtg ttgggtttac ccgcgctgat aacgccagga gagattgatc cattggcgta
    52801 cagtcgtgac tacagcgtga tgttgctggt gagcattatt tttgcgttgc tgtgctggcg
    52861 gcgctccccg caaccgggcc gtggtgtagg ggtattatta actggcggat ttatcgtatg
    52921 gctggcgatg ttgtactggt tatcgccaat actcgttgaa taactggaaa cgcattatgt
    52981 cgcacgtaga gttacaaccg ggttttgact ttcagcaagc aggtaaagaa gtcctggcga
    53041 ttgaacgtga atgcctggcg gagcttgatc aatacatcaa tcagaatttc acgcttgcct
    53101 gtgaaaagat gttctggtgt aaagggaaag ttgtcgtcat ggggatgggg aaatcggggc
    53161 acatcgggcg caaaatggcc gcaacgtttg ccagcaccgg tacaccttca tttttcgtcc
    53221 atcctggtga agccgcgcat ggtgatttag gcatggtcac cccacaggat gtggtgattg
    53281 ctatctctaa ctcaggtgaa tccagcgaaa tcacggcctt aattccagtg cttaagcgtc
    53341 ttcacgtacc gttaatctgc atcaccggtc gcccggagag cagcatggcg cgcgccgcag
    53401 atgtgcatct gtgtgttaaa gtagcgaaag aagcctgtcc gttagggctg gcaccgacca
    53461 gcagcaccac cgccacgctg gttatgggcg atgccctcgc tgtcgcgctg ttaaaagcac
    53521 gcggctttac tgctgaagat tttgcgctct cacacccagg cggcgcactg ggtcgtaaac
    53581 ttctgctgcg cgtaaacgat attatgcata cgggcgatga gatcccgcat gttaagaaaa
    53641 cggccagtct gcgtgacgca ttgctggaag ttacccgcaa aaatcttggt atgactgtca
    53701 tttgcgatga caatatgatg attgaaggca tctttaccga cggtgattta cgccgtgtct
    53761 tcgatatggg cgtggatgtt cgtcagttaa gtattgccga tgtgatgacg ccggggggaa
    53821 tacgcgtgcg ccctggcatt ctggccgttg aggcactgaa cttaatgcag tcccgccata
    53881 tcacctccgt gatggttgcc gatggcgacc atttactcgg tgtgttacat atgcatgatt
    53941 tactgcgtgc aggcgtagtg taaagattca aggataaaca acaatgagca aagcaggtgc
    54001 gtcgcttgcg acctgttacg gccctgtcag cgccgacgtt atagcaaaag cagagaacat
    54061 tcgtctgctg atcctcgacg ttgatggcgt actgtcagat ggcctgattt atatgggcaa
    54121 taacggcgaa gagctgaaag cgttcaatgt tcgtgacggt tatggcattc gttgtgcgct
    54181 cacctctgat attgaagtcg ctatcattac cgggcgaaag gctaaactgg tagaagatcg
    54241 ttgtgccaca ttggggatca ctcacttgta tcaggggcag tcaaacaaac tgatcgcctt
    54301 tagcgatctg ctggaaaaac tggcgattgc cccggaaaat gtggcttatg tcggcgatga
    54361 tctcatcgac tggccggtaa tggaaaaagt gggtttaagc gtcgccgtgg ccgatgcgca
    54421 tccactgttg atcccacgcg ccgattacgt gacgcgcatt gctggcggtc gtggcgcagt
    54481 gcgagaagtt tgcgacttat tactcctggc gcagggcaaa ctggatgaag ccaaagggca
    54541 atcgatatga gtaaagccag acgttgggtt atcattgtgc tatcactggc ggttctggtg
    54601 atgatcggca ttaatatggc cgaaaaagac gataccgccc aggtggtcgt caacaacaat
    54661 gatcccacct ataaaagcga gcatacggac acgctcgtct ataacccaga aggggcacta
    54721 agctatcgat tgattgctca acacgttgaa tattattccg atcaggccgt ttcgtggttt
    54781 acgcagccgg tacttaccac gtttgataag gataaaatcc cgacatggtc cgtaaaagca
    54841 gataaagcca agctgaccaa tgaccggatg ctctatttat atggacacgt tgaagtcaac
    54901 gcacttgtgc cggactctca acttcgcaga atcacgacgg ataacgcgca gatcaatctg
    54961 gtgacgcagg atgttacctc tgaagacctc gtcacgttat acggaacaac atttaactcc
    55021 agcggtctga aaatgcgcgg caacttacgc agcaagaacg ccgagctgat tgaaaaggtt
    55081 agaacatcct atgaaattca aaacaaacaa actcagcctt aatcttgtgc ttgccagctc
    55141 acttctggcc gccagcattc cggcatttgc cgtaaccgga gacactgatc agccgatcca
    55201 cattgaatcg gaccagcaat ctcttgatat gcaaggcaac gtggttacct ttaccggtaa
    55261 tgtcatcgtc acccagggca ccatcaaaat taatgccgac aaagtggtcg ttacccgtcc
    55321 gggcggcgaa caaggtaaag aagtgattga cggctacggt aaaccggcaa cgttctacca
    55381 gatgcaggac aacggtaaac ccgttgaagg tcacgcttcc cagatgcact acgaactggc
    55441 aaaagatttt gtcgttctga cgggtaatgc ttatctgcag caggtcgata gcaacattaa
    55501 gggcgataag atcacttacc tggtgaaaga gcagaaaatg caggccttca gcgacaaagg
    55561 taagcgcgta accaccgttc tggtgccgtc gcagctgcag gacaaaaaca acaaaggcca
    55621 gaccccggca cagaagaagg gtaattaatt cgttatggca acattaactg caaagaacct
    55681 tgcaaaagcc tataaaggcc gtcgcgtggt agaagacgtc agcctgaccg tcaactccgg
    55741 ggaaattgtc ggtctgctgg ggccaaacgg tgccggtaag accaccactt tctacatggt
    55801 tgtaggcatt gtgccgcgcg atgcgggcaa catcattatt gatgatgacg atatcagtct
    55861 gctgcctctg catgcacgcg cgcgccgcgg tatcggctat ctgccacagg aagcctccat
    55921 tttccgtcgc ctcagcgttt acgataacct gatggcggta ctgcaaattc gtgacgactt
    55981 gtctgctgaa caacgtgaag accgcgcgaa cgagctgatg gaagagtttc acattgagca
    56041 cctgcgtgac agcatggggc agtcactctc cgggggtgaa cgtcgccgtg tagaaattgc
    56101 ccgcgcactg gctgcgaatc cgaaatttat tctgctcgac gaaccgtttg ccggggttga
    56161 cccgatctcg gttatcgaca ttaaacgcat cattgaacac ctgcgcgaca gcggcctggg
    56221 cgtgctgatc actgaccaca acgtgcgtga aacactggcg gtttgtgaac gcgcttatat
    56281 cgtcagtcag gggcatttga tcgcccacgg cacgcctaca gaaatcttac aagacgaaca
    56341 cgttaagcgt gtataccttg gggaagactt cagactctga tagggtagaa gtttgcgacg
    56401 ttttagcagg agagtacgat tctgaacatg aagcaaggtt tgcaactcag gcttagccaa
    56461 caactggcga tgacgccaca gctccaacag gcaattcgtc tgttgcagtt gtcgacgctg
    56521 gaacttcagc aggagctaca gcaggcgctg gagagtaatc cgctgcttga gcaaatcgac
    56581 actcatgaag aaatcgacac ccgcgaaacg caagacagtg aaacgctgga caccgccgac
    56641 gcgctcgaac aaaaagagat gccggaagag ctgccgctcg atgccagttg ggacaccatt
    56701 tacaccgctg gtacaccatc cggcaccagc ggtgactaca ttgacgatga gctgccggtc
    56761 tatcagggcg aaacgacgca gaccttgcag gattacctga tgtggcaggt cgagctgaca
    56821 ccgttttccg acactgaccg cgctattgct acctctatcg tcgatgccgt tgatgacacc
    56881 ggttatctga ctgtcccgct ggaagatatt ctcgaaagta tgggcgatga agagatcgac
    56941 atcgacgagg ttgaagccgt ccttaagcgg atccaacggt ttgatccggt cggtgtagcg
    57001 gcaaaagatc tgcgtgactg cctgctaatc caactctccc aattcgataa gaccacgccg
    57061 tggctggaag aggccagatt gatcattagc gatcatctcg atctgttagc caatcacgac
    57121 ttccgcactt taatgcgcgt cacgcgtctg aaagaagatg tgctgaaaga agccgtcaat
    57181 ctgatccagt cgctcgatcc gcgccccggg cagtcgatcc agactggcga acctgagtat
    57241 gtcattccag atgtgctggt gcgtaagcat aacggtcact ggacggtaga actcaacagt
    57301 gacagcattc cgcgtctgca aatcaaccag cactacgcct cgatgtgcaa taacgcgcgc
    57361 aacgatggtg acagccagtt tatccgcagc aatctgcagg atgccaaatg gttgatcaag
    57421 agtctggaaa gccgtaacga tacgctactg cgcgtgagtc gctgtatcgt tgaacagcag
    57481 caagccttct ttgagcaagg tgaagaatat atgaaaccga tggtactggc cgatatcgcc
    57541 caggctgtcg aaatgcatga atcgacgata tctcgcgtca ccacgcaaaa atacctgcat
    57601 agtccacgag gcatttttga actgaagtat ttcttttcca gtcacgtcaa taccgagggc
    57661 ggcggcgaag cttcctccac ggcgattcgt gcactggtga agaaattaat cgcggcggaa
    57721 aacccagcga aaccgttgag cgacagcaag ttaacctctt tgctgtcgga acaaggtatc
    57781 atggtggcac gccgcactgt tgcgaagtac cgagagtctt tatccattcc gccgtcaaac
    57841 cagcgtaaac agctcgtttg acccaaccga taaggaagac actatgcagc tcaacattac
    57901 cggaaataac gtcgagatca ccgaggcact gcgcgaattt gttacagcta aatttgccaa
    57961 acttgagcaa tattttgacc gaatcaacca ggtctatgtt gttctgaaag tggagaaagt
    58021 cacccacacc tcagatgcaa cactgcatgt aaacggtggt gaaattcatg ccagcgcgga
    58081 aggtcaggac atgtacgctg ccattgatgg cttaattgat aagctggcac gccagttgac
    58141 caaacataaa gataaactga aacaacacta attgtccggg caattagcat gtgcatggcg
    58201 gcctgttgtg cggcacaacg ggccatttgt acggttaatg ctccgagcct gttccactgt
    58261 ttgagtgggc aggttcttag gtgaaattat gacaaataat gatacaactc tacagcttag
    58321 cagtgttctt aacagggaat gtacgcgaag ccgcgtccac tgtcagagca aaaaacgcgc
    58381 cctggaaatc atcagcgagc tggcggcgaa acaacttagc ctgccgcctc aggtggtttt
    58441 tgaagctatc ctgacgcggg aaaaaatggg cagtaccggt atcggcaatg gtattgccat
    58501 tccgcatggc aaactggaag aagatactct gcgcgccgtt ggcgttttcg ttcagctcga
    58561 aacgcctata gctttcgatg ccatcgacaa ccaaccggtg gacttacttt ttgccctgct
    58621 ggtgccggca gaccaaacta aaacgcacct gcatactctg tcgctggtgg cgaaacgtct
    58681 ggcggacaaa accatctgcc gccgtttacg cgcagcccag agcgatgaag agctgtatca
    58741 aatcattacg gataccgaag gtactccgga tgaagcgtag ttattcggta atgtctcttt
    58801 tagacgttgt gaggagaaac agtacatggt actgatgatc gtcagcggac gttcaggttc
    58861 aggtaaatct gtcgccctgc gtgcgctgga agatatgggt ttttactgcg tggataacct
    58921 tcccgtagtg ttgttacccg atctggctcg aactctggcc gatcgcgaga tttctgccgc
    58981 cgtcagcatt gatgttcgta atatgccaga gtcaccagaa atattcgaac aggcgatgag
    59041 taacctgcct gacgctttct caccgcaact actgttcctg gatgccgacc gtaatacctt
    59101 aattcgtcgt tacagtgaca cgcgccgact gcatccgctt tccagcaaaa acctgtcgct
    59161 ggaaagtgct atcgacaaag aaagcgattt gctggagcct ctgcgttcgc gagcggatct
    59221 gattgtcgat acctcagaaa tgtccgttca cgagctggct gaaatgctgc gtacccgtct
    59281 gctgggtaaa cgtgaacgcg aactgaccat ggtctttgag tctttcggct tcaaacacgg
    59341 tatccctatc gatgcagatt acgtctttga cgtgcgcttc ttgccgaacc cgcactggga
    59401 tccgaaactg cgtccaatga caggtcttga taaacctgtc gccgcgttcc tcgaccgcca
    59461 cacagaagta cacaatttta tctaccagac gcgaagctat cttgagctat ggttacctat
    59521 gctggaaacc aacaaccgta gctacttgac ggtcgccatt ggttgtaccg gcgggaagca
    59581 ccgttcggtg tatattgcag agcaactggc agactacttc cgctcgcgcg gtaaaaacgt
    59641 ccagtcacgc catcgtacgc tggaaaaacg taaaccatga ccgtcaagca aactgttgaa
    59701 atcacaaaca agctgggcat gcatgcccgg cctgcaatga agctgtttga attaatgcag
    59761 ggttttgacg ctgaagtgct gttacgtaat gacgaaggca ccgaggccga agccaacagc
    59821 gttattgcgc tgctgatgtt ggattctgcc aaaggacggc agattgaagt tgaagcgacc
    59881 ggcccacagg aagaggaagc actggccgcc gttatcgccc tctttaactc tggttttgat
    59941 gaagattaat cttcatcact ttgacataca actcccttca aactcccccc gacaataaga
    60001 aaatcacgta ctgaaatcgt tctcaatcaa cgtcatttgt acattttgtg cgcttttcac
    60061 tttcagaaga accttaagaa aaccctaaga ggcattgttt aggttttgtt taagttaatc
    60121 gaccatactg gagatcgtca gaaaatattt ccaggagatg gcatgattcg cttatctgaa
    60181 caaagtcccc tgggcaccgg aagacatcgg aaatgttatg cgcatccgga agatgcccaa
    60241 cgctgtatca agattgtcta ccatcgtggc gatggtggcg ataaagagat ccgccgtgag
    60301 ttaaagttct acgcgcatct tggtcgccgg ttaaaagact ggagtggaat accgcgctat
    60361 cacggtaccg ttgagacgga ttgcggaacc ggttatgtct acgatgtgat tgcagatttt
    60421 gacggtaaac cctccattac gctgaccgaa tttgctgaac aatgccgtta cgaagaagac
    60481 attgcacaac tgcgccagct actgaaacag ctgaagcgtt atttgcagga taaccgtatc
    60541 gtgacgatgt cgctgaagcc gcagaatatc ctctgtcacc gcatcagtga gtcagaggtt
    60601 atcccggtcg tctgcgacaa cattggtgaa agcacattga ttccactggc gacctggtcg
    60661 aagtggtgtt gtctgcgtaa gcaagagcgg ttatggaaac gatttatcgc ccaaccggcg
    60721 ctggctattg ccctgcagaa agatctgcaa cctcgtgaga gcaaaacgct ggccctcact
    60781 tcgcgcgaag cttaatccag ctggtgttgt tgcataaacg gctcaccgcc taactgatac
    60841 atctgccgta aaatccactc ctgacggcta cgcacgtagc ccgatggcga ggagactttg
    60901 aaacgaagtg gattaggtaa tacagctgcc agtaatgcag cttccgaccg ggtaagtttg
    60961 ctcgcgggtt tgtggaaata acgttgtgcc gcagcttcga cgccaaacac accgtcgcca
    61021 aattcggcga tattcaggta aaccgtcagg atacgctttt tgctccagac tgtttctatc
    61081 cccagcgtta atccagcttc cagccctttt cgtacccagc tacgcccatc ccataaaaag
    61141 agatttttgg ccgtctgctg agaaatcgtt gaagcaccgc gaatacggtt ttcattgcgc
    61201 tcgttgtgcg ccagggcttt ctcaatggaa gcgacatcaa agccccagtg ctcaggaaat
    61261 ttctgatctt ctacggcaat caccgccagt cccatccacg gcgagatttg atccatactg
    61321 acccagtcag aatgtgccac gtaacgaaaa ttgccatgca gccaggcgct gacctgtcgc
    61381 tcgaccatta ccgctgagaa gggaacaggc gcaacactaa acaacgcgat gcccccgccc
    61441 cagaaaacgg cgaggacaac cattaaccgc aaaagaaaac ggcgaacgaa actaaacacc
    61501 gttaagcggc ttttactcat tcagccagaa ccagcacgcg ggaaaccagc ttatcaatgc
    61561 cgctcgccgc ttctgcaatg ttctgcgcca gcatatatgc tggggtggtg acaatcttat
    61621 tgtcttcatc aaccacgata tcatcgacag gacagggcac atgctccgcg cccatctctt
    61681 ccagcacttc tgcggtatcg atatcagtac cgatggtcaa acgcagcggg aaatcgaaaa
    61741 ttttcggcag catcgccggg gcaatacaca taaaaccaag cggttttccg gcctgatgca
    61801 tcgcttgtgc cagcgccttt aattcacggt caacggagca ttcgctacca agagtggcaa
    61861 aattgcttaa attcttcgcc gcgccaaacc cccccggcac aatcaacgca tccagttcag
    61921 cggcatcggc ctgggccaga ggacggattt caccgcgcgt tattcgtgcc gcttcaatca
    61981 gcacgttgcg cgtttccgtc atcgcttcgc cagttaaatg gttgataaca tcaacctgct
    62041 gcttatccgg tgcaaagcag accgcctgcg caccgctgcg tgaaatagct aacaacgtca
    62101 acaccgcttc atgaatttca gaaccgtcat agacgccgca tccgctcaga attacgccaa
    62161 ttttcttcat tgtgatcatc ctttcgcaat tgactgaaac acattaattt ttttaataaa
    62221 aatggtacgc atcacacatt taactgattc atgtaacaaa tcatttaagt tttgctatct
    62281 taactgcgtg cggcctgaaa aacagtgctg tgcccttgta actcatcata ataatttacg
    62341 gcgcagccaa gatttccctg gtgttggcgc agtattcgcg caccccggtc tagccggggt
    62401 cattttttag tggcttttgc cacccacgct ttcagcactt ctacgtcgtg acgccactct
    62461 tctttcatct cttcaatcca ttcaccgacg ttatcttccc aggcaggaag gtcaggagac
    62521 tgaatttgct gacccagctg ttgcagatgg cgtaacccca ctgaccccgc cgcaccttta
    62581 attttatgtc cttcctcaac aatgcctttt ttatcctgcg ccgtcagatt cgactccagc
    62641 acgctgacat agcccggcat catcttctca aacaccgcta acccgtcggt gatcagcttc
    62701 ggtcctacaa gttcgagata ctgttccagc atgggaatat cgagcaatgc ttctgattta
    62761 ctgttctctt ctgtcgtcac cgtactctcc tcatcatcct gggtatccca gaatttcttg
    62821 atcatcgcgg ttagcgccgg aaccgaaagc ggcttactca gcacatcatc cattccagca
    62881 ttgaggtact cttgtttgtc tttcagcacg ttagcggtta aggccaccag cggcggtaaa
    62941 tcctcgcgcg gataacgctt cgtcagttca cgagagatat ccagcccggt catatctggc
    63001 aactgaatat ccagcaacac caggtcgtat tcgcccggtt taaacatctc cagcgccgcc
    63061 ttgccggtca tggcgacatc aacgctgtta cctaattttt ccagcacaga acgcgcaaca
    63121 atcacgttca gttcaatgtc ttccaccagc agcacattca gcgccggtaa aggcatatcg
    63181 tcttcatcaa acgcatcatc gacctcttct gctaccgacg gtgcgtggat cgtcaacgta
    63241 aaggttgaac ctttgccctg ttcgctggta accgtaatat cgccgcccat atttttcgcc
    63301 agacgacgag aaacggccag accaataccg gtgccggtgg caggtttacc gccatgactg
    63361 tctttcacct ggtaatacat ggcgaaaatt ttatccagct catcctgcgg aatgccgata
    63421 ccagagtctt ccacttcaaa atgcagcata tcgccttcat cgtagcgcac gcgcacggta
    63481 acctggcctt gctgggtgaa tttgacggcg ttactgatga ggttccacag gatctgccgt
    63541 aaacgcgtcc cgtcggtaat gacctgatgc ggtaatggca gcgtcggctc caggttaaag
    63601 cgcaatcctt tttgttgcgc ctgcaatgcg gagagatttt ccagatcggc aaggaagctg
    63661 gtgaaatcaa ccggttgatt atcaagctgg accttgcgcc gttccatctt atccatgtcg
    63721 ataatatcgt taaagatatt ccccagcgtg acggccgaaa catggatggt cttgagatat
    63781 ttttcctgct cggcggtgag ttcggtatcc agcagaatgc ggctcagacc gacgataccg
    63841 ttcagcggtg tacgcaattc gtgactgatg gtggagataa acgtcgtttt gtcgcggctg
    63901 gcccgttcaa gcgcatcctg ataccgctta cgctcggtaa tgtcgcgacc aaagcccatc
    63961 aaaccgtgac gtttacccac gcggtcgtag tacggcactt tacggatttc aaagcaggct
    64021 ttgcgcccgt ccgggtaatc cagccactgt tcataggtca gtgacacatt atgacggaac
    64081 actttttcat cggtttcaat gacttttgcg gcggcttccg gtgagtaaac atcagcaggt
    64141 ttcaggtgaa ccagttgttt ttcgcttttt ccggtcagca gctccatcgc gcggttacag
    64201 ccggaaaact ctttatcttc gttacgataa aaaaccaggt cgggtgaagc atcaaggaag
    64261 gaacgtaaga atgaggattg ctgctcgagc tgaatttgtg tctcttcgcg ctctttgatt
    64321 tcaattttca gttggccgaa ggtttcctgc agttctgctt ccgctttttc acgaacggca
    64381 atttcctgat ttagctgggc aatattatct tttaactgaa cgttgaggct caaatcgcgc
    64441 tcgcgcatct cctccagttt ttgcaccagc cgtgacagac gttgtcgtga ctcctccagt
    64501 tgctcgacga ccaccgatag aaagtagacc gcccacggcg taatcagcaa accaaagaag
    64561 atagaacgaa taacatcaat gctttcgacc tgaccatgca gcaccatggt taccgccatt
    64621 tgtaccacaa tggcaagaac gacgagggcc agcgccagca acattgagaa gcgcaccaga
    64681 cctaacttca tcatcaggtc aacataatac tgcgccagca gacgaatttg cttcattagg
    64741 gaattccttc acgacaacct gccccaataa tacccaattc tgacagttac gttataaatt
    64801 gtgtgagaaa tgcggaattc ccctcaacga cctactccgt tggaggtagc cacggacgtc
    64861 ccagcgccga tccctgtacg ccatgttcat tcagatagcg atccagctcg accattcccg
    64921 tccatcgatt ttcgcaccac agcggagcaa gcagcgtcgg gcgacgggcg ctggcggaaa
    64981 tgcggtggta gatcacttcc ggtggcgtat ggcgaatcat ttctccggca gtgagcgtgt
    65041 aatcctccag ttcaataccg ttcaaacgcc ccgcttccca ggctttcgcc ataatgctgc
    65101 ctttcacaat atgcagcgga tgcagcttta tgccgtccac gccggtttca accactcgct
    65161 ccagcgtttg caaacattca gcctgccctt cgccaggcag gccgacaatt aagtgggaac
    65221 acaccttcag cccgcgctga cgtgccagct gggttgtacg ctgataacag gcaaaatcat
    65281 ggccgcggtt gatgcgatgc agtgttttgt cgtgggcggt ttgtagcccc agctccagcc
    65341 acacttcgta accctggtcc ttatattcgc aaagcagatc cagcaccgca tccggcacgc
    65401 agtccgggcg ggtaccaaca cacaaaccga caatattggc ctggctcacc gcctgctgat
    65461 acatagaacg cagcacctga acttccgcaa aggtgctggt atacgcctga aagtaggcca
    65521 gatagcgttt agcgcggtta actaaattcg cctggtgcgc cagttgctcg gcaatggaac
    65581 gatgctgctg cgcttcatcg gcaaacgagg ccacattaca gaatgtgcag ccaccacgcc
    65641 cgatggtacc gtcacggtta gggcagctaa aaccgccgtg cagcgtcagc ttatgcacct
    65701 tttgcccata acgacgggtg agatcaccac caaacatatt gactaatttc tgtaactgca
    65761 taatctgata gaccgcgcct tgaaaagagg ccaaagcctg ccatttttag ctcaattcgg
    65821 cgatgacctg gatcaatcgt cccggcctgc ttttatcaac tgcataatca atcaaaatta
    65881 ccgaaatttc atgcataatc acataaatca cttttgctta tcttgtgtca gattttttta
    65941 tctccttatg gattttaggc aaaaacagta gcatgaaacg tcattaccaa ttaaggcagt
    66001 ataaaatgct ggttttctcg tcagttcaag gcaggataag ggttaacacg cctttatgac
    66061 agtcaggaat tgactgtttc tctaacgact tcccttttag ccttaaagat aaaatccatt
    66121 ttaatttcag tcgtttaata aagaattttg cgctaaagca catttctgta ccaataagct
    66181 tgccatttga cctgtatcag ctttcccgat aagttggaaa tccgctggaa gctttctgga
    66241 tgagcagcct gctcatcata tttatgcagt aattgagatc ccctcttcac cgtattaacc
    66301 gatgcgaaaa ggacaacaag ggggcgaatg cgaggcgcgc gtatgacacg caacccccgt
    66361 cgccacgctc tttctgtgcc cgtgcgcagc ggttcggaag tggggttccc gcagagcctg
    66421 ggggaggttc acgatatgtt gtacgataaa tcccttgaga gggataactg tggtttcggc
    66481 ctgatcgccc acatagaagg cgaacctagc cacaaggtag tgcgtactgc aatacacgca
    66541 ctggcccgca tgcagcaccg tggcgcgatt ctcgccgatg gtaaaaccgg tgacggttgc
    66601 ggcttgctgt tacaaaaacc ggatcgcttt tttcgcatcg ttgcgcagga gcgcggctgg
    66661 cgtttagcga aaaactacgc cgtcgggatg ctattcctga ataaagatcc tgaactcgcc
    66721 gctgccgcac gccgcatcgt tgaagaagaa ctgcaacgcg aaaccttgtc gattgtgggc
    66781 tggcgtgatg tccccactaa cgaaggcgtg ctgggtgaaa tcgccctctc ctctctgccc
    66841 cgcattgagc aaattttcgt taacgctccg gcaggctggc gtccgcggga tatggagcgc
    66901 cgtctgttta tcgcccgccg ccgcattgaa aagcgtctcg aagccgacaa agacttctac
    66961 gtctgtagcc tgtcgaatct ggtgaacatc tataaaggtc tgtgtatgcc ggcggatctg
    67021 ccgcgctttt atctggatct tgcggacctg cgtctggaat cggccatctg cctgttccac
    67081 cagcgcttct ccactaacac cgtaccgcgc tggccgctgg cgcagccgtt ccgctacctg
    67141 gcgcataacg gtgaaatcaa caccatcacc ggtaaccgcc aatgggcgcg tgcgcgtacc
    67201 tataaattcc agacgccgct tatccctgat ctgcacgacg ccgcaccgtt cgtcaacgaa
    67261 accggctctg actccagttc gatggataac atgctggaac tgctgctggc aggcgggatg
    67321 gatatcatcc gtgccatgcg tctgttagtg ccacccgcct ggcagaacaa cccggatatg
    67381 gacccggaac tgcgtgcctt ctttgacttt aactccatgc atatggagcc gtgggatggc
    67441 ccggcgggca tcgtgatgtc cgacggtcgt tttgccgcct gtaacctcga ccgtaacggt
    67501 ctgcgtccgg cgcgctacgt catcaccaaa gataagctca tcacctgcgc ctctgaagtc
    67561 ggtatctggg attaccagcc tgacgaagtg gtcgaaaaag gccgcgtcgg gccaggcgaa
    67621 ctgatggtta tcgacacccg cagtgggcgt attctgcact cggcagaaac cgatgacgat
    67681 ctgaaaagcc gccatccata taaagagtgg atggagaaaa acgtccgccg acttgtaccg
    67741 tttgaagatc tgcccgatga agaagtgggt agccgcgaac tggacgacga cacgcttgcc
    67801 agctaccaga aacagtttaa ctacagcgcg gaagagctgg actccgtaat tcgcgtactg
    67861 ggcgaaaacg gtcaggaagc ggtcggttcg atgggcgatg ataccccatt cgccgtgctc
    67921 tccagtcagc cgcgcattat ttacgactac ttccgccagc agtttgccca ggtgactaac
    67981 ccgccaatcg acccgctgcg tgaagcgcat gttatgtcgc tcgccaccag tatcggtcgt
    68041 gaaatgaacg tcttttgcga agcagagggc caggcgcacc gtttaagctt taaatcgccg
    68101 attctgctct actccgattt caaacagctc acgacgatga aagaggagca ctaccgcgca
    68161 gatacgctgg atatcacctt tgacgtcact aaaaccacgc tcgaagcgac agtcaaagag
    68221 ctgtgcgaca aagccgaaaa aatggtacgt agcggcaccg tgctgctggt gctctccgac
    68281 cggaatatcg ctaaagatcg cctgccggtt ccagccccga tggcggttgg cgcgatccag
    68341 acccgtctgg tcaatcaaag cctgcgttgc gatgccaaca tcatcgtcga aaccgccagc
    68401 gcccgcgatc cgcaccactt cgccgtgttg ctgggcttcg gcgcgacggc tatttatcca
    68461 taccttgcct atgaaacgct gggccgcctg gtagacaccc atgcgattgc caaagattat
    68521 cgtaccgtga tgctcaacta ccgtaacggc atcaacaaag gcttgtacaa aatcatgtcc
    68581 aaaatgggca tctccaccat cgcctcttac cgctgctcga aactgtttga agcggtcggt
    68641 ctacacgatg atgtagtggg cctgtgcttc cagggggcgg tcagccgcat tggtggggcg
    68701 agctttgaag acttccagca ggatctgctg aacctgtcga aacgtgcctg gctggcgcgt
    68761 aagcccatca gccagggcgg cctgctgaaa tacgtccacg gcggcgaata ccacgcctac
    68821 aacccggacg tggtgcgcac gctgcaacaa gcggtacaaa gcggcgagta cagcgactat
    68881 caggaatacg cgaagctggt taatgagcgt ccggcaacca cgctgcgcga tctgctggca
    68941 attacgccgg gtgaaaacgc ggtcaacatt gctgatgttg aaccggcaag cgaactgttt
    69001 aaacgcttcg ataccgctgc gatgtctatc ggcgcgttaa gcccggaagc ccacgaggcg
    69061 ctggcggaag cgatgaacag catcggcggt aactcaaact ccggtgaagg cggtgaagat
    69121 ccggcgcgct acggcaccaa caaagtgtcg cgcatcaagc aggtggcttc cggtcgcttt
    69181 ggggttactc cggcgtatct ggtcaatgcc gacgtcattc agattaaagt cgcccagggc
    69241 gcgaagccgg gcgaaggcgg tcagttgccg ggtgataaag tcactcctta catcgccaaa
    69301 ctgcgctatt cggtgcccgg agtgacgctg atctccccgc cgccgcacca cgatatctac
    69361 tctatcgagg acttagcgca gctcattttc gacctcaagc aggttaaccc gaaagcgatg
    69421 atctccgtga agctggtttc cgaaccgggc gtaggcacca tcgcgactgg cgtggcaaaa
    69481 gcttatgccg atttaatcac catcgcaggc tatgacggcg gcaccggcgc aagtccgctt
    69541 tcatcggtga aatacgcagg ctgtccgtgg gagctggggc ttgttgaaac ccagcaggcg
    69601 ctggttgcta acggcttgcg tcacaagatc cgtttgcagg tcgatggcgg cctgaaaacg
    69661 ggtgtcgata tcatcaaggc ggcgattctc ggcgcagaaa gcttcggctt cggcactggc
    69721 cctatggtgg cgctcggctg taaatatcta cgtatttgcc atctgaacaa ctgcgcaacg
    69781 ggtgtagcaa ctcaggatga caaactgcgt aagaaccact atcacggcct gccgttcaag
    69841 gttaccaatt actttgaatt tattgcccgt gaaacccgcg agctgatggc acagctgggc
    69901 gtaacgcgtc tggtggatct gattggtcgc accgacctgc tgaaagaact ggacggtttc
    69961 accgccaaac agcaaaaact ggcgctgtcg aagctgctgg agaccgccga accgcatcca
    70021 ggtaaggcac tctactgcac cgaaaacaac ccgccgtttg ataacggcct gctgaacgcg
    70081 cagttgctgc aacaggcgaa accgtttgtc gatgaacgcc agagcaaaac cttctggttc
    70141 gatatccgca ataccgatcg ctccgtgggc gcgtcgcttt caggctatat cgcccagacg
    70201 cacggcgatc aggggctggc agccgatcct atcaaagcgt acttcaacgg caccgcaggc
    70261 cagagtttcg gtgtatggaa cgcgggcggc gtggaactgt acttgaccgg ggatgccaac
    70321 gactatgtcg gtaaaggcat ggcgggcggc ttaatcgcca ttcgtcctcc ggttggttcc
    70381 gccttccgca gccatgaagc aagtattatc ggcaacacct gcctgtatgg cgcgaccggt
    70441 ggtcgtctgt atgccgcagg ccgcgcgggt gaacgtttcg gcgtgcggaa ctccggtgct
    70501 atcaccgtgg tagaaggcat tggcgacaac ggctgtgaat atatgacggg cggtattgtc
    70561 tgtattctgg gtaaaaccgg cgttaacttc ggtgcgggca tgaccggcgg cttcgcttac
    70621 gttctcgatg aaagcggtga tttccgcaaa cgcgttaacc cggaactggt cgaggtctta
    70681 agcgttgacg atctggcgat ccatgaagag catctgcgcg gtcttatcac cgagcatgtt
    70741 cagcatactg gctctcagcg cggtgaagag attctggcga actggtcaac cttcgccact
    70801 aaatttgcgc tggttaaacc gaagtccagt gatgtaaaag cactgctggg tcatcgtagt
    70861 cgtagcgcag cagagttgcg cgtgcaggcg cagtaagggg tagcaacaat gagtcagaat
    70921 gtttatcaat ttatcgacct gcagcgcgtt gatccgccaa agaaaccgct gaagatccgc
    70981 aaaattgagt ttgttgaaat ttacgagccg ttttccgaag gccaggccaa agcgcaggct
    71041 gaccgctgcc tgtcgtgcgg caacccatac tgcgagtgga aatgtccggt acacaactac
    71101 atcccgaact ggctgaagct cgccaacgag gggcgtattt ttgaagcggc ggaactgtcg
    71161 caccagacca acaccctgcc ggaagtttgc ggacgagtct gcccgcaaga ccgtctgtgc
    71221 gaaggttcct gcactctgaa cgatgagttt ggcgcggtga ccatcggcaa cattgagcgt
    71281 tatatcaatg ataaagcgtt cgaaatgggc tggcgtccgg atatgtccgg cgtgaagcaa
    71341 accggtaaaa aagtggcgat tatcggcgca ggcccggcag gtctggcgtg tgcggatgtc
    71401 ctgacgcgta acggcgtaaa agccgttgtc ttcgaccgtc atccagaaat tggcgggctg
    71461 ctgaccttcg gtattccggc cttcaagctg gaaaaagagg taatgacgcg tcgccgtgaa
    71521 atcttcaccg gcatgggtat tgaattcaaa ctcaataccg aagtgggccg cgacgtgcag
    71581 ctggacgatc tgctgagtga ttacgatgcc gtcttccttg gcgtcgggac ttatcagtca
    71641 atgcgcggcg ggctggaaaa cgaagacgcc gatggcgtgt acgcagcgct gccgttcctt
    71701 atcgccaaca ccaaacagtt aatgggcttt ggtgaaaccc gcgacgaacc gttcgtcagc
    71761 atggaaggca aacgcgtggt ggtccttggc ggtggcgaca ctgcgatgga ctgcgtgcgt
    71821 acgtccgtgc gccagggagc gaagcacgtt acctgtgcct atcgtcgtga tgaagagaac
    71881 atgccgggtt cccgccgcga agtgaaaaac gcgcgggaag aaggcgtaga gttcaaattc
    71941 aacgtccagc cactggggat tgaagtgaac ggtaacggta aagtcagcgg cgtaaaaatg
    72001 gtacgtaccg aaatgggcga accggatgcc aaaggccgtc gccgcgcgga gatcgttgca
    72061 ggttccgaac atatcgttcc ggcagatgca gtgatcatgg cgtttggttt ccgtccacac
    72121 aacatggaat ggctggcaaa acacagcgtc gagctggatt cacaaggccg catcatcgcc
    72181 ccggaaggca gcgacaacgc cttccagacc agcaacccga aaatctttgc tggcggcgat
    72241 atcgtccgtg gttccgatct ggtggtaacc gctattgccg aaggtcgtaa ggcggcagac
    72301 ggtattatga actggctgga agtttaagct ctgtcccagc gccgcctcct cagaaaagag
    72361 tcggcgctga aattctcctt tctttctgga aagcgcctcg cataatctga agaaaaccaa
    72421 tgtaatacgc tcatctggat tgttaccggt agatgcccgg cgtatagctg attccattat
    72481 tcaacgcagc accatataaa gggacagata atggaatcgc tctcagagag aacctcaaca
    72541 ggctaccagc aaatccacga cggtattatt cacctggtcg atagcgcccg gacggaaacg
    72601 gtacgtagcg ttaacgcgct gatgaccgcg acatactggg aaattggccg acgaattgtc
    72661 gaatttgaac aaggtggcga ggcaagggct gcgtatggtg cgcagctaat caagcgacta
    72721 tcaaaggatt taagtctaag gtataagcgt gggttctctg caaaaaactt acgccaaatg
    72781 aggctttttt acctcttttt tcaacatgtt gaaattcgcc agacagtgtc tggcgaatta
    72841 acaccattgg gaattccgca gacaccatct gcagaattcc catcggcaaa gatttggcag
    72901 acgctgtctg ccaaattgag aggctgtttg atatttccac agcttttgta ttgcatctcc
    72961 tattttcggt ttgtatattc atgcaaataa acgaaggaaa gttgccatga tttacattga
    73021 acagcttgaa ttaatccata aaagtggtga tgtgttgtat ccagtaaaaa taaccagaaa
    73081 atcatcagga aaaactgcct tccaccttgt tccattcggc ttaaataaga ctcacgacct
    73141 tctagaggtg gaagatgctt cagaagcaat tcggctggtg attgatgaac gtcattccat
    73201 tcgctgcagt acacttacag caactattac gaataaaaag ggcaagcgaa ttaagcggac
    73261 aggtatatac agtatcaagg gtgttaacat aaaagaatac aacgtcaggt aatggcacag
    73321 agcggtccgt cagttcaggt ttggctctgt gccaaagatg ggtcaggtca cacctgatct
    73381 aataaattta agccattaaa tcagccttaa ccttcaccac caccttttta atctcacccg
    73441 gctcgccaac aacgcatccc ggcttatgcg gctctcctgg cataaacacg gcgaacattc
    73501 ccggttttaa gatgatggtt tgctcgtttt caatggcgct gcaaagctga taatcatcct
    73561 catggtggaa ctcttcacac tgacgcgcgg tgcctgccat gccaaacaga atccgttcct
    73621 caccgtttaa taacagctgg atatcaatgt attgctcgtg caattccgct tttttctcga
    73681 cgggcgattg agtgttaaac gtcatgacat tcataaagat attgtcgccc tgtaattcgt
    73741 aacgacccgg cgctttttct tgcggtctgg cagctaatgc cagcgttaac gcgtcctgta
    73801 acgcaggatg taacccagca gacggtaatg actgtacttc acccatcatc ataatttttc
    73861 tccctgggcc aacagcgcag ccccaagtaa acctgcatcg tggcggtagt gcgccgccag
    73921 taaatcaaca tgaaatgccg ctggctcctg cgccagatac gtttccacca gcgccagata
    73981 cccttctgcc agcccaacgc tgccaccgac caccacgcac tggcaatcag ttgtggcttt
    74041 aatatcagcg atcagccttg ccagcacatg tgcggagcgg tgaatcagtt gcagcgcctg
    74101 ctcgtcgccc tgcccggcgc gtgtgaaaat agttctcgca tccgcgccca tgagttcacc
    74161 ttgcgctgcc gctgcaatac cgcgaccaga agcaatcgct tccacgcaac ctgtgcgtcc
    74221 acagccgcag actgggccgt gtggatcggc aagcgtatgc ccgatatgcc ccgccagacc
    74281 gccagggccg gtaagcagtt taccgccgct cactacaccg ccgccgacgc cagtggaaac
    74341 ggtgataaac accatctcgc ttacatcgcc ttccagcgcc tgatactccg cccatgctgc
    74401 agcctgcgcg tcgttaatgg caatggtcgg caaatcggta agttgttcca gcgttttgac
    74461 taacggaaag tgtagcaatc caccaagatt atgcggattc agcgccagca agctgccgtc
    74521 gcgaataatc ccggttgaag cgatggcaac ccgctgcgca tgagcttgca acggagagac
    74581 taatgcggat aaggcatcac gcaaggcttc tggtgtctgg ctggctggcg taggaagttc
    74641 acgacgatcg cggatctgcc cgtcagcgcc aatcagcgcg gcggcaagtt tagtaccgcc
    74701 gatatcaatc gccagcgtgg tcatagcacc gcctttttca tcgctgtgtt gtaccactga
    74761 caaatgtgtt caagacgcgt aatggcagaa ccgaccgtca ccgcccacgc gccgtggcgc
    74821 atcgcatctg ccgcctgagc aggcgtgttg taacgccctt cggcaatcac cctacatccg
    74881 gcgtcgctca acgttttcac cagcgccaga tccggctctt ctggcgtttc aggcgtggta
    74941 tagccagaaa gcgtagtgcc aataatttcg gctcccagct tttggcatgc caggccgtct
    75001 tccggcgttg agcagtcggt catcgccagt aaaccgtgat ggtgaatacg tgccagcagc
    75061 gtttcaacag gcaccggacg cgggcggtcg gtgccgtcaa tggcgataat gtccgcgccc
    75121 gcctgcgcca gcgcatcaac atcttcaata taggccgtga tgcgtaccgg agaatcctcc
    75181 agatcgcgtt tcacaattcc aataatcggc acgctcacca ccgcacgcgt ggcttgcaga
    75241 tttgccacac cttcaatgcg aatggcaacc gcgcccgccc gttctgccgc taatgccatg
    75301 gcggcgacga tttcgggttt atcgagcggg ctgtccggaa ccggctggca ggagacaatc
    75361 aggccaccgt tagcagcgat tttttgatcc agttgtgcaa gtaacgacat acatcttccc
    75421 ttagcgaaag gcccggtaca tagaccgggc aacaggatta acttttggtt ttgactaaat
    75481 cgtttttggc gctgccaaac ggcacggcac cgctgaatgg tttaccgtcg atagcgtcat
    75541 gagtacgcaa cgcttccggg cgcaaccaac gctgaacgcg agaaggcata tccagcccaa
    75601 tcagcaggat caccacgaac gtcagactga acgagagcga tgccagcgca gtacccagat
    75661 ccagacgttg agcgatcaac gcgccgatga ttggggccag tgcaccgccc aatgcgccaa
    75721 cgttgtaggt aaagcccagg cccgctgcac gctggtcggt atcgaaataa ccgccaatca
    75781 gtttcggtaa gatcccggcg atcccttgtc caagcatttg ctggaagaac agtaacagac
    75841 cgagcaccca gacgtttgcg ccgccaatcg caaataccgg aataatcaac agctgcgagg
    75901 ccaacaggct acaaacgtag gctttgcggg ttcccagcca gtcaccgagg aagccgccga
    75961 cacagcaccc caccgcagcc ccaaagccgc taaagaacag cacattggcg acagtatgcg
    76021 ggttataagc cagatcggtt ttcagatacg ttggcagcag cgcctgaatc ggccatgagt
    76081 agaggaaagc aaacaacacg accaccatca gcattacgcc cgttggccag cgtttacccg
    76141 tactttgcac cataaagctg ataaagattg cggcgcataa cagcccaaga acagcgacga
    76201 tcgcggcatt ttgcaggtta ccggcgaagc aaaaccacag cgcagtagcc gccgccagtg
    76261 tcattacgat attggcaatg cgatgttcac cacggtagag aatatctacc atcgtgcgta
    76321 ctggtgcttt acctgcatgt ttctctttcc agtcttccgc ttccgggatg tttttacgca
    76381 gccagagagc aaagatgata ggcaaaatgc cgataaagaa cagcgcacgc cagccccaga
    76441 ccggaaccac caggctatag acctgagcgg caacgacggc ccccacagag aagcctgaaa
    76501 tcagaaaacc actggctttg ttacgcagat gttttggcca gctttcaatg acataggtgg
    76561 cgctggaacc gtattcaccc gccatcccca tgccgatgac cagacgcgcg ataaacatgg
    76621 tgatgtagcc tggcgcaaag ccgcaggcca gcgtcccggc cgagaagaga acgatgctgg
    76681 tgaccattgc cagacgacgc ccgtagcggt cacccatagc gccgagcatc aggccgccga
    76741 accagcgaga gataaaggct gcggagatca gacttgccgc ctgcaccgtc gtcagcccga
    76801 attcaccctg tacttcggtg agtaccaggg cgattaaaac gaaatcaaaa ccgtcaagca
    76861 gatatcccaa ccaggcagcg gaaaatgcgc gccattgtgc acggttgaga tggcgatacc
    76921 acgggatatt ctgggttgta gtactcattg tgagtctccc gcggtgggcg atgcccacac
    76981 gctttggtat aaaaattgta gggtacagat gcgtttattt cccctcaccc ggtaggggcg
    77041 agcgagggga aacaactcac ccgcgctctt gcatcaactg ctgggccagc gccttcagtt
    77101 ctggcagata tttttcatct accggtccaa acggtttgcg gcacagcggc acagaaacga
    77161 catccatata atggaggaca gttttcaggc cgcggaatac gcccgttttg atcagtaaat
    77221 caatgacttt attgcattca gtttgcagtt tctgcgcggt ctggatatcg ccttctttca
    77281 gcgccttaac gatcccctga tagcgccagc ccatgatgtt gtaggtacta ccgataccac
    77341 catcagcgcc cgccagcaga ccagaggcga agatttcgtc gtaaccgtta tagagcacaa
    77401 gatcaggatg ttcacgacgg atctgctcca tctgatagag atcgccagag gtctgtttca
    77461 gcgcacctac gccaggcaat ataacaagtg tgttgatctg atccagggtc agttttaccc
    77521 cactcagggc tggaatgttg tacaccacca tcggcaaacc atccgccgaa tcaataattg
    77581 cccgatagtg atcgcagtgt tcttcaaagc tgaaaggata gtagaacggc gtgacggcgg
    77641 agacggcatc gaagccataa cgtttagccg atgccgcaag ttgttggctt tcggcggtgc
    77701 tgacgcaacc gacgtgggcg atgagtttaa tcttaccttt cgcctcttcg gcgacgattt
    77761 ccagtacctg ttcacgctcg gaaaggcttt gtacaaaggc ctcgccggtc gaaccaccca
    77821 cgtataaacc gtcgatgccc tgctgaatat tgaactgaac caggcgacgc agactcgctt
    77881 tatccagtgc ttgttgttgg tcaaaaggag tcaggagtgc agccattacg ccacgtaaat
    77941 tcgttgccat aaatacctct gaagtgatgc ttgtctgata aacgatatac ctttatacct
    78001 gttataccag atcaattaag caacacccca tacagaaagc ttataatgcg atctgcttca
    78061 ctaaagtggc attatttctt tttgttggtg gtctgaccga aagcgtgcca ggtagcagag
    78121 acgctgttga gatgcgattg caacgcacga tcggcttcgt caggatcatg acggcggatc
    78181 gcatcaacga tcgcaatatg ctgttgataa ctaacgttgt tatgttcgtg cagtgcctga
    78241 tcggtaaccg ttgggcgtgc ggcaataagc cagtcgagca gggcaacgtg gatcgccatg
    78301 aagattgggt taccggggat ctccgccagc acgcggtgga aatcaacgtc tgaacgaatg
    78361 aatgccgcgt tgttatccag cgactgactg ttgatttcca gtgcttttgc cagcaaatcg
    78421 atttgctcat cggtggcatg ttcagccgca tagcgcacca gactggattc aaagaacaga
    78481 cgtaattgtt cgaaatgggc aatcccaccg ggatgagaaa ggaaatcttt cgccatgccg
    78541 gaaagctcac cgatgatagt gtccgcagaa ggacgcgaga cgcgagcgcg ttcgccgttg
    78601 tttatttgca ccagaccttt gcgttttaac gctgccagcg cttcacgcac cgaaggacgc
    78661 ccgacgttaa agaacgccat cagttcgcgt tcagacggta attgttcacc ttcgccaaat
    78721 tcacgacggc ggatcatctg ttccagctct tcttccacca tttcggagag ttttttacgc
    78781 gccagcgggc ggctacgcaa gttgcgacca attgcaggtg aagaatcttc ggtttgcgaa
    78841 tcaaatgcgt tcataaggcc cattctgtaa ggtcagtgtg attaacatca tcagtgacat
    78901 cctatcacag gattgaaagt aggggaaaat ggcagggttt tctctttgtg cctcatcatt
    78961 accataatta acggaataat tgactattgc gaaaaagtaa tgtaacgcag ataaaaacat
    79021 cccgattgaa ttatttataa gactattcac aagcattatg aa
//
LOCUS       DAZKHC020000008        75302 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZKHC020000008 DAZKHC020000000
VERSION     DAZKHC020000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 75302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.008"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..552
                     /locus_tag="ACHOSM_RS03375"
                     /old_locus_tag="pgaptmp_000675"
                     /pseudo
     CDS             1..552
                     /locus_tag="ACHOSM_RS03375"
                     /old_locus_tag="pgaptmp_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896819.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            655..1158
                     /locus_tag="ACHOSM_RS03380"
                     /old_locus_tag="pgaptmp_000676"
     CDS             655..1158
                     /locus_tag="ACHOSM_RS03380"
                     /old_locus_tag="pgaptmp_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HGU4557256.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1225..2682)
                     /gene="nuoN"
                     /locus_tag="ACHOSM_RS03385"
                     /old_locus_tag="pgaptmp_000677"
     CDS             complement(1225..2682)
                     /gene="nuoN"
                     /locus_tag="ACHOSM_RS03385"
                     /old_locus_tag="pgaptmp_000677"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HGU4557257.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNDPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2689..4218)
                     /gene="nuoM"
                     /locus_tag="ACHOSM_RS03390"
                     /old_locus_tag="pgaptmp_000678"
     CDS             complement(2689..4218)
                     /gene="nuoM"
                     /locus_tag="ACHOSM_RS03390"
                     /old_locus_tag="pgaptmp_000678"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HGU4557258.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4382..6223)
                     /gene="nuoL"
                     /locus_tag="ACHOSM_RS03395"
                     /old_locus_tag="pgaptmp_000679"
     CDS             complement(4382..6223)
                     /gene="nuoL"
                     /locus_tag="ACHOSM_RS03395"
                     /old_locus_tag="pgaptmp_000679"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HGU4557259.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWV
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6220..6522)
                     /gene="nuoK"
                     /locus_tag="ACHOSM_RS03400"
                     /old_locus_tag="pgaptmp_000680"
     CDS             complement(6220..6522)
                     /gene="nuoK"
                     /locus_tag="ACHOSM_RS03400"
                     /old_locus_tag="pgaptmp_000680"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HGU4557260.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6519..7073)
                     /gene="nuoJ"
                     /locus_tag="ACHOSM_RS03405"
                     /old_locus_tag="pgaptmp_000681"
     CDS             complement(6519..7073)
                     /gene="nuoJ"
                     /locus_tag="ACHOSM_RS03405"
                     /old_locus_tag="pgaptmp_000681"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HGU4557261.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7085..7627)
                     /gene="nuoI"
                     /locus_tag="ACHOSM_RS03410"
                     /old_locus_tag="pgaptmp_000682"
     CDS             complement(7085..7627)
                     /gene="nuoI"
                     /locus_tag="ACHOSM_RS03410"
                     /old_locus_tag="pgaptmp_000682"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HGU4557262.1"
                     /translation="MTLKELLVGIGTQVRSIWMIGLHAFAKRETRMYPEERVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7642..8619)
                     /gene="nuoH"
                     /locus_tag="ACHOSM_RS03415"
                     /old_locus_tag="pgaptmp_000683"
     CDS             complement(7642..8619)
                     /gene="nuoH"
                     /locus_tag="ACHOSM_RS03415"
                     /old_locus_tag="pgaptmp_000683"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HGU4557263.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8616..11342)
                     /gene="nuoG"
                     /locus_tag="ACHOSM_RS03420"
                     /old_locus_tag="pgaptmp_000684"
     CDS             complement(8616..11342)
                     /gene="nuoG"
                     /locus_tag="ACHOSM_RS03420"
                     /old_locus_tag="pgaptmp_000684"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HGU4557264.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11395..12732)
                     /gene="nuoF"
                     /locus_tag="ACHOSM_RS03425"
                     /old_locus_tag="pgaptmp_000685"
     CDS             complement(11395..12732)
                     /gene="nuoF"
                     /locus_tag="ACHOSM_RS03425"
                     /old_locus_tag="pgaptmp_000685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HGU4557265.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12729..13229)
                     /gene="nuoE"
                     /locus_tag="ACHOSM_RS03430"
                     /old_locus_tag="pgaptmp_000686"
     CDS             complement(12729..13229)
                     /gene="nuoE"
                     /locus_tag="ACHOSM_RS03430"
                     /old_locus_tag="pgaptmp_000686"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HGU4557266.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNITPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13232..15034)
                     /gene="nuoC"
                     /locus_tag="ACHOSM_RS03435"
                     /old_locus_tag="pgaptmp_000687"
     CDS             complement(13232..15034)
                     /gene="nuoC"
                     /locus_tag="ACHOSM_RS03435"
                     /old_locus_tag="pgaptmp_000687"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HGU4557267.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15128..15790)
                     /gene="nuoB"
                     /locus_tag="ACHOSM_RS03440"
                     /old_locus_tag="pgaptmp_000688"
     CDS             complement(15128..15790)
                     /gene="nuoB"
                     /locus_tag="ACHOSM_RS03440"
                     /old_locus_tag="pgaptmp_000688"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HGU4557268.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15806..16249)
                     /gene="nuoA"
                     /locus_tag="ACHOSM_RS03445"
                     /old_locus_tag="pgaptmp_000689"
     CDS             complement(15806..16249)
                     /gene="nuoA"
                     /locus_tag="ACHOSM_RS03445"
                     /old_locus_tag="pgaptmp_000689"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HGU4557269.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16880..17818)
                     /gene="lrhA"
                     /locus_tag="ACHOSM_RS03450"
                     /old_locus_tag="pgaptmp_000690"
     CDS             complement(16880..17818)
                     /gene="lrhA"
                     /locus_tag="ACHOSM_RS03450"
                     /old_locus_tag="pgaptmp_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HGU4557270.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHKPWQYSPMSAPEGDDSLLIERDIE"
     gene            18738..19955
                     /gene="alaA"
                     /locus_tag="ACHOSM_RS03455"
                     /old_locus_tag="pgaptmp_000691"
     CDS             18738..19955
                     /gene="alaA"
                     /locus_tag="ACHOSM_RS03455"
                     /old_locus_tag="pgaptmp_000691"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HGU4557271.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20039..20638
                     /gene="yfbR"
                     /locus_tag="ACHOSM_RS03460"
                     /old_locus_tag="pgaptmp_000692"
     CDS             20039..20638
                     /gene="yfbR"
                     /locus_tag="ACHOSM_RS03460"
                     /old_locus_tag="pgaptmp_000692"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4557272.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNTERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLESRRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20697..22529)
                     /locus_tag="ACHOSM_RS03465"
                     /old_locus_tag="pgaptmp_000693"
     CDS             complement(20697..22529)
                     /locus_tag="ACHOSM_RS03465"
                     /old_locus_tag="pgaptmp_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGU4557273.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22616..23266)
                     /gene="hxpA"
                     /locus_tag="ACHOSM_RS03470"
                     /old_locus_tag="pgaptmp_000694"
     CDS             complement(22616..23266)
                     /gene="hxpA"
                     /locus_tag="ACHOSM_RS03470"
                     /old_locus_tag="pgaptmp_000694"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HGU4557274.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23277..23771)
                     /locus_tag="ACHOSM_RS03475"
                     /old_locus_tag="pgaptmp_000695"
     CDS             complement(23277..23771)
                     /locus_tag="ACHOSM_RS03475"
                     /old_locus_tag="pgaptmp_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HGU4557275.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23853..24308)
                     /gene="yfbV"
                     /locus_tag="ACHOSM_RS03480"
                     /old_locus_tag="pgaptmp_000696"
     CDS             complement(23853..24308)
                     /gene="yfbV"
                     /locus_tag="ACHOSM_RS03480"
                     /old_locus_tag="pgaptmp_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HGU4557276.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24646..25848
                     /gene="ackA"
                     /locus_tag="ACHOSM_RS03485"
                     /old_locus_tag="pgaptmp_000697"
     CDS             24646..25848
                     /gene="ackA"
                     /locus_tag="ACHOSM_RS03485"
                     /old_locus_tag="pgaptmp_000697"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGU4557277.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25923..28067
                     /gene="pta"
                     /locus_tag="ACHOSM_RS03490"
                     /old_locus_tag="pgaptmp_000698"
     CDS             25923..28067
                     /gene="pta"
                     /locus_tag="ACHOSM_RS03490"
                     /old_locus_tag="pgaptmp_000698"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU4557278.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28257..29777
                     /gene="yfcC"
                     /locus_tag="ACHOSM_RS03495"
                     /old_locus_tag="pgaptmp_000699"
     CDS             28257..29777
                     /gene="yfcC"
                     /locus_tag="ACHOSM_RS03495"
                     /old_locus_tag="pgaptmp_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HGU4557279.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29810..30352)
                     /gene="yfcD"
                     /locus_tag="ACHOSM_RS03500"
                     /old_locus_tag="pgaptmp_000700"
     CDS             complement(29810..30352)
                     /gene="yfcD"
                     /locus_tag="ACHOSM_RS03500"
                     /old_locus_tag="pgaptmp_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HGU4557280.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30410..30961)
                     /gene="yfcE"
                     /locus_tag="ACHOSM_RS03505"
                     /old_locus_tag="pgaptmp_000701"
     CDS             complement(30410..30961)
                     /gene="yfcE"
                     /locus_tag="ACHOSM_RS03505"
                     /old_locus_tag="pgaptmp_000701"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HGU4557281.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31017..31661)
                     /gene="yfcF"
                     /locus_tag="ACHOSM_RS03510"
                     /old_locus_tag="pgaptmp_000702"
     CDS             complement(31017..31661)
                     /gene="yfcF"
                     /locus_tag="ACHOSM_RS03510"
                     /old_locus_tag="pgaptmp_000702"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HGU4557282.1"
                     /translation="MSKPAITLWSDAHCFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31797..32444
                     /gene="yfcG"
                     /locus_tag="ACHOSM_RS03515"
                     /old_locus_tag="pgaptmp_000703"
     CDS             31797..32444
                     /gene="yfcG"
                     /locus_tag="ACHOSM_RS03515"
                     /old_locus_tag="pgaptmp_000703"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HGU4557283.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32501..32863
                     /gene="folX"
                     /locus_tag="ACHOSM_RS03520"
                     /old_locus_tag="pgaptmp_000704"
     CDS             32501..32863
                     /gene="folX"
                     /locus_tag="ACHOSM_RS03520"
                     /old_locus_tag="pgaptmp_000704"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HGU4557284.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32884..33777
                     /locus_tag="ACHOSM_RS03525"
                     /old_locus_tag="pgaptmp_000705"
     CDS             32884..33777
                     /locus_tag="ACHOSM_RS03525"
                     /old_locus_tag="pgaptmp_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HGU4557285.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EDAGFAFRWYDLEEALADVVR"
     gene            complement(33825..34715)
                     /gene="rpnB"
                     /locus_tag="ACHOSM_RS03530"
                     /old_locus_tag="pgaptmp_000706"
     CDS             complement(33825..34715)
                     /gene="rpnB"
                     /locus_tag="ACHOSM_RS03530"
                     /old_locus_tag="pgaptmp_000706"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HGU4557286.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34912..35685)
                     /gene="hisP"
                     /locus_tag="ACHOSM_RS03535"
                     /old_locus_tag="pgaptmp_000707"
     CDS             complement(34912..35685)
                     /gene="hisP"
                     /locus_tag="ACHOSM_RS03535"
                     /old_locus_tag="pgaptmp_000707"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HGU4557287.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35693..36409)
                     /locus_tag="ACHOSM_RS03540"
                     /old_locus_tag="pgaptmp_000708"
     CDS             complement(35693..36409)
                     /locus_tag="ACHOSM_RS03540"
                     /old_locus_tag="pgaptmp_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4557288.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36406..37092)
                     /gene="hisQ"
                     /locus_tag="ACHOSM_RS03545"
                     /old_locus_tag="pgaptmp_000709"
     CDS             complement(36406..37092)
                     /gene="hisQ"
                     /locus_tag="ACHOSM_RS03545"
                     /old_locus_tag="pgaptmp_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HGU4557289.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37182..37964)
                     /gene="hisJ"
                     /locus_tag="ACHOSM_RS03550"
                     /old_locus_tag="pgaptmp_000710"
     CDS             complement(37182..37964)
                     /gene="hisJ"
                     /locus_tag="ACHOSM_RS03550"
                     /old_locus_tag="pgaptmp_000710"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HGU4557290.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38185..38967)
                     /gene="argT"
                     /locus_tag="ACHOSM_RS03555"
                     /old_locus_tag="pgaptmp_000711"
     CDS             complement(38185..38967)
                     /gene="argT"
                     /locus_tag="ACHOSM_RS03555"
                     /old_locus_tag="pgaptmp_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HGU4557291.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAEMTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39233..39802)
                     /gene="ubiX"
                     /locus_tag="ACHOSM_RS03560"
                     /old_locus_tag="pgaptmp_000712"
     CDS             complement(39233..39802)
                     /gene="ubiX"
                     /locus_tag="ACHOSM_RS03560"
                     /old_locus_tag="pgaptmp_000712"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HGU4557292.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39897..41414)
                     /gene="purF"
                     /locus_tag="ACHOSM_RS03565"
                     /old_locus_tag="pgaptmp_000713"
     CDS             complement(39897..41414)
                     /gene="purF"
                     /locus_tag="ACHOSM_RS03565"
                     /old_locus_tag="pgaptmp_000713"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGU4557293.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41451..41939)
                     /gene="cvpA"
                     /locus_tag="ACHOSM_RS03570"
                     /old_locus_tag="pgaptmp_000714"
     CDS             complement(41451..41939)
                     /gene="cvpA"
                     /locus_tag="ACHOSM_RS03570"
                     /old_locus_tag="pgaptmp_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HGU4557294.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42198..42860)
                     /gene="dedD"
                     /locus_tag="ACHOSM_RS03575"
                     /old_locus_tag="pgaptmp_000715"
     CDS             complement(42198..42860)
                     /gene="dedD"
                     /locus_tag="ACHOSM_RS03575"
                     /old_locus_tag="pgaptmp_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HGU4557295.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42850..44118)
                     /gene="folC"
                     /locus_tag="ACHOSM_RS03580"
                     /old_locus_tag="pgaptmp_000716"
     CDS             complement(42850..44118)
                     /gene="folC"
                     /locus_tag="ACHOSM_RS03580"
                     /old_locus_tag="pgaptmp_000716"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HGU4557296.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     QKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44188..45102)
                     /gene="accD"
                     /locus_tag="ACHOSM_RS03585"
                     /old_locus_tag="pgaptmp_000717"
     CDS             complement(44188..45102)
                     /gene="accD"
                     /locus_tag="ACHOSM_RS03585"
                     /old_locus_tag="pgaptmp_000717"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGU4557297.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45258..45917)
                     /locus_tag="ACHOSM_RS03590"
                     /old_locus_tag="pgaptmp_000718"
     CDS             complement(45258..45917)
                     /locus_tag="ACHOSM_RS03590"
                     /old_locus_tag="pgaptmp_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4557298.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46000..46812)
                     /gene="truA"
                     /locus_tag="ACHOSM_RS03595"
                     /old_locus_tag="pgaptmp_000719"
     CDS             complement(46000..46812)
                     /gene="truA"
                     /locus_tag="ACHOSM_RS03595"
                     /old_locus_tag="pgaptmp_000719"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGU4557299.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     LVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46812..47825)
                     /locus_tag="ACHOSM_RS03600"
                     /old_locus_tag="pgaptmp_000720"
     CDS             complement(46812..47825)
                     /locus_tag="ACHOSM_RS03600"
                     /old_locus_tag="pgaptmp_000720"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU4557300.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47891..49027)
                     /gene="pdxB"
                     /locus_tag="ACHOSM_RS03605"
                     /old_locus_tag="pgaptmp_000721"
     CDS             complement(47891..49027)
                     /gene="pdxB"
                     /locus_tag="ACHOSM_RS03605"
                     /old_locus_tag="pgaptmp_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HGU4557301.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIVFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49126..50121
                     /gene="flk"
                     /locus_tag="ACHOSM_RS03610"
                     /old_locus_tag="pgaptmp_000722"
     CDS             49126..50121
                     /gene="flk"
                     /locus_tag="ACHOSM_RS03610"
                     /old_locus_tag="pgaptmp_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HGU4557302.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50118..51296)
                     /locus_tag="ACHOSM_RS03615"
                     /old_locus_tag="pgaptmp_000723"
     CDS             complement(50118..51296)
                     /locus_tag="ACHOSM_RS03615"
                     /old_locus_tag="pgaptmp_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4557303.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51605..52825)
                     /gene="fabB"
                     /locus_tag="ACHOSM_RS03620"
                     /old_locus_tag="pgaptmp_000724"
     CDS             complement(51605..52825)
                     /gene="fabB"
                     /locus_tag="ACHOSM_RS03620"
                     /old_locus_tag="pgaptmp_000724"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HGU4557304.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            52984..54990
                     /gene="mnmC"
                     /locus_tag="ACHOSM_RS03625"
                     /old_locus_tag="pgaptmp_000725"
     CDS             52984..54990
                     /gene="mnmC"
                     /locus_tag="ACHOSM_RS03625"
                     /old_locus_tag="pgaptmp_000725"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HGU4557305.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDDEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55111..55389)
                     /locus_tag="ACHOSM_RS03630"
                     /old_locus_tag="pgaptmp_000726"
     CDS             complement(55111..55389)
                     /locus_tag="ACHOSM_RS03630"
                     /old_locus_tag="pgaptmp_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HGU4557306.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(55423..55971)
                     /gene="epmC"
                     /locus_tag="ACHOSM_RS03635"
                     /old_locus_tag="pgaptmp_000727"
     CDS             complement(55423..55971)
                     /gene="epmC"
                     /locus_tag="ACHOSM_RS03635"
                     /old_locus_tag="pgaptmp_000727"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HGU4557307.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55971..56780)
                     /locus_tag="ACHOSM_RS03640"
                     /old_locus_tag="pgaptmp_000728"
     CDS             complement(55971..56780)
                     /locus_tag="ACHOSM_RS03640"
                     /old_locus_tag="pgaptmp_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGU4557308.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56780..57604)
                     /gene="mepA"
                     /locus_tag="ACHOSM_RS03645"
                     /old_locus_tag="pgaptmp_000729"
     CDS             complement(56780..57604)
                     /gene="mepA"
                     /locus_tag="ACHOSM_RS03645"
                     /old_locus_tag="pgaptmp_000729"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HGU4557309.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57608..58693)
                     /gene="aroC"
                     /locus_tag="ACHOSM_RS03650"
                     /old_locus_tag="pgaptmp_000730"
     CDS             complement(57608..58693)
                     /gene="aroC"
                     /locus_tag="ACHOSM_RS03650"
                     /old_locus_tag="pgaptmp_000730"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGU4557310.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58728..59660)
                     /gene="prmB"
                     /locus_tag="ACHOSM_RS03655"
                     /old_locus_tag="pgaptmp_000731"
     CDS             complement(58728..59660)
                     /gene="prmB"
                     /locus_tag="ACHOSM_RS03655"
                     /old_locus_tag="pgaptmp_000731"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU4557311.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59826..60377
                     /gene="smrB"
                     /locus_tag="ACHOSM_RS03660"
                     /old_locus_tag="pgaptmp_000732"
     CDS             59826..60377
                     /gene="smrB"
                     /locus_tag="ACHOSM_RS03660"
                     /old_locus_tag="pgaptmp_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HGU4557312.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60499..61371)
                     /locus_tag="ACHOSM_RS03665"
                     /old_locus_tag="pgaptmp_000733"
     CDS             complement(60499..61371)
                     /locus_tag="ACHOSM_RS03665"
                     /old_locus_tag="pgaptmp_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HGU4557313.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGTVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(61358..61882)
                     /locus_tag="ACHOSM_RS03670"
                     /old_locus_tag="pgaptmp_000734"
     CDS             complement(61358..61882)
                     /locus_tag="ACHOSM_RS03670"
                     /old_locus_tag="pgaptmp_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4557314.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61879..62349)
                     /locus_tag="ACHOSM_RS03675"
                     /old_locus_tag="pgaptmp_000735"
     CDS             complement(61879..62349)
                     /locus_tag="ACHOSM_RS03675"
                     /old_locus_tag="pgaptmp_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4557315.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(62346..62894)
                     /locus_tag="ACHOSM_RS03680"
                     /old_locus_tag="pgaptmp_000736"
     CDS             complement(62346..62894)
                     /locus_tag="ACHOSM_RS03680"
                     /old_locus_tag="pgaptmp_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4557316.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(62869..63621)
                     /locus_tag="ACHOSM_RS03685"
                     /old_locus_tag="pgaptmp_000737"
     CDS             complement(62869..63621)
                     /locus_tag="ACHOSM_RS03685"
                     /old_locus_tag="pgaptmp_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4557317.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            complement(63641..66283)
                     /gene="yfcU"
                     /locus_tag="ACHOSM_RS03690"
                     /old_locus_tag="pgaptmp_000738"
     CDS             complement(63641..66283)
                     /gene="yfcU"
                     /locus_tag="ACHOSM_RS03690"
                     /old_locus_tag="pgaptmp_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HGU4557318.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMARTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDCYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(66365..66928)
                     /locus_tag="ACHOSM_RS03695"
                     /old_locus_tag="pgaptmp_000739"
     CDS             complement(66365..66928)
                     /locus_tag="ACHOSM_RS03695"
                     /old_locus_tag="pgaptmp_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4557319.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLASGDSLATSYKSGMGIDQKIAKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(67603..68088)
                     /gene="sixA"
                     /locus_tag="ACHOSM_RS03700"
                     /old_locus_tag="pgaptmp_000740"
     CDS             complement(67603..68088)
                     /gene="sixA"
                     /locus_tag="ACHOSM_RS03700"
                     /old_locus_tag="pgaptmp_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HGU4557320.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(68291..70435)
                     /gene="fadJ"
                     /locus_tag="ACHOSM_RS03705"
                     /old_locus_tag="pgaptmp_000741"
     CDS             complement(68291..70435)
                     /gene="fadJ"
                     /locus_tag="ACHOSM_RS03705"
                     /old_locus_tag="pgaptmp_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HGU4557321.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(70435..71745)
                     /gene="fadI"
                     /locus_tag="ACHOSM_RS03710"
                     /old_locus_tag="pgaptmp_000742"
     CDS             complement(70435..71745)
                     /gene="fadI"
                     /locus_tag="ACHOSM_RS03710"
                     /old_locus_tag="pgaptmp_000742"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HGU4557322.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTVFDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(71926..72210)
                     /locus_tag="ACHOSM_RS03715"
                     /old_locus_tag="pgaptmp_000743"
     CDS             complement(71926..72210)
                     /locus_tag="ACHOSM_RS03715"
                     /old_locus_tag="pgaptmp_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HGU4557323.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            72582..73922
                     /gene="fadL"
                     /locus_tag="ACHOSM_RS03720"
                     /old_locus_tag="pgaptmp_000744"
     CDS             72582..73922
                     /gene="fadL"
                     /locus_tag="ACHOSM_RS03720"
                     /old_locus_tag="pgaptmp_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HGU4557324.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESKGKAWLFGTNFNYAF"
     gene            74288..>75302
                     /gene="yfdF"
                     /locus_tag="ACHOSM_RS03725"
                     /old_locus_tag="pgaptmp_000745"
     CDS             74288..>75302
                     /gene="yfdF"
                     /locus_tag="ACHOSM_RS03725"
                     /old_locus_tag="pgaptmp_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HGU4557325.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGVIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENAKSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQTE
                     YQNE"
BASE COUNT        18913 a        20275 c        18609 g        17505 t
ORIGIN      
        1 tatcgccaac gccccgacac gtaagtcctg agcaattaga aaaaacagtt cgttatctta
       61 cacaaactgt gcatccacgt aatgccgaca atattgataa tctgaatagg tccgtagagt
      121 atataaaaga gttctttgtc agtagcggtg ccagagttac cgcgcagggc gtccccgatt
      181 ggctattacg actctgcgcc tggtagccaa gattaccctt acccgcaatg tcctggcttt
      241 atcccgatcg gggggacttt attgcctggt cggcagaata caggatatca acgccgttcg
      301 tcaggtaaaa acggcattgt tatcatcccg ggatttatct gtttattcta tgaatgcccc
      361 atggtttatt cccggtattg atttctctga ccacctgaat tattggcaac acgatattcc
      421 cgccgtaatg attactgaca ccgcttttta tcgtaataaa caataccact tgcccggtga
      481 tacagcagac agattgaatt atcagaaaat ggctcagatg gtgttataac cttgttacac
      541 aacagtaaat aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg tacattttat
      601 tttccctcgt ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa ataaatggga
      661 atgattggtt attttgcaga aattgactca gaaaagatta accaactgct tgagtctact
      721 gaaaaacctt tgatggataa tatacatgac acgctttcag gattacgccg actggatatt
      781 gataaacgct gggatttttt acactttggg ctgacaggca cctctgcttt tgatcccgcg
      841 aagaatgatc cgctgagtcg tgccgtgctt ggtgaacaca gcctcgaaga tggcatagat
      901 ggcttcctgg gattgacctg gaatcaggag ttagccgcga caatcgaccg actggaatca
      961 ctggatcgaa gtgagctgcg taaacagttt tccattaagc gactcaatga aatggagatt
     1021 tatccgggag tgaccttttc agaagagtta gaggggcaac tctttgcttc gatcatgctt
     1081 gatatggaaa agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg
     1141 acggttattg ttgggtgatg gtgtcagggc accaaaaaaa accgccgaac atgtcggcgg
     1201 ctttctgact tacaaactga tagcttacat cagcggcatt gctaaccgca caatgctaat
     1261 cagcggttgt ggccatacac ccagcaccag taccaacagt gcagagatca gcaccacgat
     1321 accgcctgcg ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggggcgtg
     1381 caggtacagg ctcaccgcca cgcgcaggta gtagtaaagg ccgattgccg aaccgacaac
     1441 cacggcgccc accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt
     1501 accaataaag cccagcgtca tcggaatacc ggccagagac agcatcatca ccgtcatcac
     1561 cgccgcgagg atcgggcgat gccagaacag accgcggtaa gagaacaggg aatcagcatc
     1621 cgggccacgg tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctgaa
     1681 cagataaccg gccaggtaaa ctcctaccgc ttccatcgac atctcgcccg tttgcagcgc
     1741 aatcagcgcc accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt
     1801 gatattggtc tggctcagcg ccatcaggtt accgaagatg atagaggcaa aggcgataat
     1861 cgccagcact acccgaatcg cttcgctgtc acccaccggt gcgtagagga acagacgcat
     1921 caccacaccg aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg
     1981 cgcgccctgg tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc
     2041 aaggccaaca atcatcaggc cgaaacctgc cagcaacagc ggatcgttga gcataccgtc
     2101 gccgaggttt ttacccagcg cgacaaacga caggtcgcca gactgcgcat acaccagcgc
     2161 cataccgaac agcaggaaag aagacgctgc ggcagaaagg atagtgtatt tgatactggc
     2221 ttccagcgaa cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat
     2281 cagttcgata ccgaggaaca gagacgccag atggttggca ttcgccagca ggatcccgcc
     2341 cagcgcggca attaacacca acaggtagaa ctcatccttg ttgtcgttat agccttcaag
     2401 ccacgggtag gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag
     2461 catggcgaaa ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcccg cctggccaac
     2521 aaaccagagc gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa
     2581 atgattgcgt cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag
     2641 caacggtagc agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt
     2701 agtagtaacg gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc
     2761 cagaatcggc tgcggataga agcccagcag taccagcagc accaccagca acaggatcat
     2821 aaacagctca cgcagcgaca tccctggcag ttcctggctg gcaatctggc ttttcgcttt
     2881 accgaagtaa gcgcgatgta acatcgccag cgaataaaca gatgcaaaga ccagcccaaa
     2941 ggtagagata acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc
     3001 gccgacgaag ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag
     3061 tgctggcagc catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg
     3121 gatacgttca taaagctgac cgcagagaat aaacagaccc gccgccgaca agccgtgcgc
     3181 aatcatctgg attaccgcgc cctggtaggc caactggctg ccggtgtaga ttgcaatcag
     3241 cacgaagccc atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgggc
     3301 gaaggccatc cacgcaccgt agaagatgcc gataacaccc agccacatag caattggcgc
     3361 gaactctgcc gacgcgttcg ggaacagcgg cagggagaaa cgcagcaaac cgtaagcggc
     3421 agttttcagc aagatccccg cgaggtcaac ggaaccggcg gtcggagcct gggagtgcgc
     3481 atccggcagc cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa
     3541 gcccagcatt aacaggtatt ccgcaccact ggacattggc gtattcagca gctcttcata
     3601 gttgaaggtc cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcaat
     3661 caacatcacc agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg
     3721 cgttttaccg tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag
     3781 catcatttcc cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac
     3841 gccgcccagg atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt
     3901 ccacgaacat agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga
     3961 cagcccgtca atggcgagat gaatagagat accaaaacgc gggatccacg gcatgtcgaa
     4021 ttcagactgc cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca
     4081 cagttgcagc gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgcggcac
     4141 cttgacgcca aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat
     4201 tagccagggt agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt
     4261 ttcaacgaat tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcgccg
     4321 cgaaggcagc ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa
     4381 ctcaacgcag taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca
     4441 cataccagcg cagataaccg ttctcgctta acagcagacc tttacctgca aagcgggaaa
     4501 gaaccgccgg gatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac
     4561 ccaggaacgg cttgacgaac actttgtcgt acagccagtc aaagccccag gcgttgtacc
     4621 accaggtact cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagtacgtt
     4681 tacccagcca cagccaggct gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa
     4741 tttccagggt caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca
     4801 gcggcggtac aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca
     4861 ggctgtgagt tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga
     4921 tgaaaatcat acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca
     4981 tcagattgat atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc
     5041 ccgcagtgac cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa
     5101 ccagcggaat agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg
     5161 ccagaatgac ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca
     5221 agtggaaaat cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct
     5281 ggctcatggt agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac
     5341 cggccagcag cagcgtaacc gccccgacaa tacccaccag atgcagaact tccggcgtca
     5401 tcaggaacag gccgtgggta cgggcgatca ggtagacacc cgcggttacc atcgttgcgg
     5461 cgtggatcag cgcggagaca ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca
     5521 acggcaactg cgcagattta ccgaccgcac cgcccagcag catcagcgtc acccacatca
     5581 gcatgttatt gccgtcagca aagtgcgctg gtgccagttc caccatttcg cggaagttca
     5641 gggtgcccag ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac
     5701 gggtcacgac gaacgctttc attgccgctg cgccattctt cggatcggtg taatagaaac
     5761 cgatcagcag ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt
     5821 tatcggcgag aaccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc
     5881 gagagtagcc ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca
     5941 caccagtgac caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga
     6001 tgttaaagtc gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt
     6061 tagcgaagaa atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa
     6121 cgccgacgat cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga
     6181 agccaatcaa tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac
     6241 tgaatcgatg ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat
     6301 actcgcttct gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg
     6361 accccagtag cttccggcga ccacaaaggc cagcgcggag gcgttaatca tgatttccag
     6421 accaatcagc ataaacagca gattgcgacg gataaccaga ccggttaagc caagaacgaa
     6481 taagattgcc gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt
     6541 cttttcgcgc tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg
     6601 acgtggaagg ccacaaccag acccgcgagc agcagcatag aagccagttc caccgccagt
     6661 acgtaaggcc cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata
     6721 ccctgatcat taacaccaag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa
     6781 attgccggac caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa
     6841 ccgcccaggt tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag
     6901 acgataattt ccagcgcacc ggcgaagtaa gcgcccagtg agaagaacac cccggaaatc
     6961 gccagcagcg aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact
     7021 cgcaaggtcg caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct
     7081 ctccttacgg taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct
     7141 tatctttgcc gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc
     7201 ccggaccgga gatcagcaga tcctcttttt cgtaaaccag atcctggcgc ttgtattccc
     7261 ccatttcgaa atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac
     7321 cacagaaaat gcagcgtgag aagttgatgc ggaaaaattc cgggtaccag cgaccgtctt
     7381 tggtttctgc tttttgcagc gagatacagc cgaccgggca ggctaccgcg cagaggttac
     7441 aggctacgca acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac
     7501 ggggcggcag atagacccgc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt
     7561 gcaggccgat catccagata ctacgaacct gggtgccgat acctactaac aattctttta
     7621 aggtcatggt cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag
     7681 caagttaatc agcgtcagcg gcaggcagat tttccagccg aaggacatta cctggtcata
     7741 acgcggacgc ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt
     7801 cagcgcgaac cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt
     7861 caccatcaat gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa
     7921 cttcataccg gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg
     7981 gtcaaacggg tgacggtgac ataccgccac gcccgcgatg gcaaaggtaa taaaaccaaa
     8041 gaattgcggg ataacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa
     8101 tgaaccggcc tgagccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag
     8161 ggtctgcgca gaagcacgca tcgcacccag caacgagtat ttgttgttac ttgaccagcc
     8221 cgcaaacagc accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat
     8281 gttcaggtcg gcaaccaccc aacccggact gactggcaca atcgcaaagg ccagcagcag
     8341 cgaggtaaag gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat
     8401 ccagtcttct ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca
     8461 gccaacacgg ttaggtccgt aacggttctg gaacagaccc agcaggcgac gttcgccaaa
     8521 gctcatgaat gccccgcagg tgacaaccac cagcaggatc accaccgctt tgaggatggt
     8581 cagcaggatc tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc
     8641 tcaagatgcg cgccagccag caccggagca ataccggaca tacccatcgg caagcccacc
     8701 tgccctgccg tcagcccttc agcgatttca accggcagcg tgaccgtgtt gccatcgtaa
     8761 ctaaaggaga cgtgtgtacc tgcgttcaca cctaacttcg cggcatccgc tgggttgagt
     8821 ttgatgtacg gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg
     8881 ctgccaaaca ggtggtaata cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt
     8941 gccggtacgc tggtgaagta atccagacca ttttcgctgg tttcaaacag acgcacgccc
     9001 ggatcgccaa agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg
     9061 gagttccagc ccggcgccca ggcaaacggc acttgcgaac ggtgcgcagt cggctggttg
     9121 ttaccttcca tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga
     9181 acgctgatat tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt
     9241 ttctgcccac gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc tgccagttcc
     9301 gggattttcg ccacaacagc gtcaatcaca tggtcgagct gcgtccagtc cacttcacgg
     9361 ctcagcaggg tgctgtgcag cgagtgtaac cagcgccagc tttccagcat gacagttttg
     9421 ctgtcgtaat aagcaggatc gtaaacctgg aagaaacgtt gggcgcggcc ttcgttgttg
     9481 atcaccgtac cgtcgctttc agcaaagctg gcagcagaaa gtaccagatg ggcgttttcc
     9541 ataatcgctg tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca
     9601 ttcacgcggg tagcggaagc gtgacgatgc agatcgtttt ccagcaccac caccgcgtcg
     9661 gcgcgtccgg tttccagttc ggtcaacgct tcttcaagcg aaccgccacc cataatgccc
     9721 agccccatgc tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct
     9781 ttcagggctt tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc
     9841 ccggagataa tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg
     9901 ctttgcagct caggttcgat accgtcaacc gctggcgcag agttatccag cgcatgggcg
     9961 atggcaaaac ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata
    10021 tcatccagac gggtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg
    10081 atgttgagga ttgccgcaat ctgccagtca gccactttct gtgctgccgc catttcgcgc
    10141 gctttacctt tcaccgcctg acgcactgcc agcgcgacgc gcgcgccggt ctgggtaacg
    10201 tcttcgccca gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa
    10261 atgccgcctt cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg
    10321 ataccggtgt agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg
    10381 ctggcacgcg gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg
    10441 ccctgcattg cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc
    10501 tgacgcggac gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg
    10561 aagtagtggt ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt
    10621 tcaccggggc tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc
    10681 atatcccatt tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg
    10741 caaatttcga ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg
    10801 aagtagacgt tgtcgtgcgc accgtaaacg cccagatctg taccgtcagc gtaatctttg
    10861 tagtaacgca cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc
    10921 cccaaatcct gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg
    10981 gtcatcacag tcatatcctg aagatggcag ttaccgccct cttcacatac cggacagtcg
    11041 tgcgggtggt tggtcatcaa ccactcgacc acgctttcac ggaactgttt cgcttcttcg
    11101 tcgtcaatgg aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga
    11161 ccacgcgtgt cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgaca
    11221 cttcccagcg ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct
    11281 tccagcaggt tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc
    11341 attagcatgc ttccagtttt ctcagtcaga gactgagcgt taatcgaaat tcggttacca
    11401 gcgctctttc agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg
    11461 tttgattccc gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac
    11521 tgcaccaggt gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc
    11581 aagtgtttcg atatcgcccg gctgaccttc accacgctcc agcgcacgca gaattttcac
    11641 gctccacggc agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa
    11701 ctcttccagg ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag
    11761 cgccgtaccc agacggcttc ccgctttacc gatactttcg aattccatcg gcaggtcaag
    11821 gtgcgcttcg gtcaggaagt cggtgcccgc gccgcctggc tgccaggctt tgaatttcag
    11881 accgtcacgc ataccaccgg cgtaatcttc gaggatctcg cgcgcggtag taccaaacgg
    11941 cagttcccac agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc
    12001 atctttactt ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac
    12061 gttacacagg gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc
    12121 cgggaagggg ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc
    12181 tgtttcttcc ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa
    12241 acctgttccc ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg
    12301 gcgcagattt actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc
    12361 tttcagcgca aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat
    12421 caagaggcgg tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg
    12481 gatgttcatg gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc
    12541 tccgccgcgc cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag
    12601 cccggtcagc gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca
    12661 cactggctgt ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat
    12721 aatgtttttc atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg
    12781 tgagtgtcct catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt
    12841 ggcagcagcg taaagcggcc atcaaatgtc gtttgccctg gtgtgatgtt cagctttttc
    12901 tcgagcgccg cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa
    12961 cggatcacat ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct
    13021 tcgacgtcgc ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc
    13081 acccagccac gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct
    13141 tcgtagtggt gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc
    13201 tcggtttgtg gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat
    13261 cgatactgcc cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa
    13321 tttgctgcaa atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac
    13381 cgtcgctggt caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt
    13441 cattggcagg catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga
    13501 tatgttgcag cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg
    13561 ggccttccgg catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt
    13621 ccactttcag cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa
    13681 agtcgaagtt ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg
    13741 tagcacgcag gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg
    13801 caacgccctg ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg
    13861 ccagacgttt cggcatccag tcgaggaact cacgcagcag gcgatcccag ccgcgcggca
    13921 ggtcgtgcgc tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg
    13981 cttccaccag atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg
    14041 cgccgacgtc ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt
    14101 cggagagcat aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct
    14161 ctaccgccag cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg
    14221 tatacggaat gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt
    14281 ggtagccgat gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac
    14341 ggaaagcccc gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcgt
    14401 tttcggtgcc gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca
    14461 gatcctgttt ggctttggtc agctcaaacg gcgagaattc ggtagcgcgc gccgggtaat
    14521 ctttacgcag cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt
    14581 gaccgtcgaa agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg
    14641 ggaacagttt ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca
    14701 tgatgtcgcg gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag
    14761 gtaacccttc gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga
    14821 cgtaaggttt cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga
    14881 tccacacaac gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac
    14941 ggttgcgcag ttcgccaatc accggatcat caagatgatc gcgggtctgc caggcgggtt
    15001 cttgcgcggt taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg
    15061 cgcagtaagc ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag
    15121 gcgcaaatta aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct
    15181 tgcgttcgcg ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg
    15241 agagcggacg acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg
    15301 cttcaggacg cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc
    15361 cctgcacaac ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga
    15421 taacccattt tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg
    15481 taaagcaggt tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt
    15541 ctgcgccaaa acgcgctacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac
    15601 agcaggaaag accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt
    15661 cattgagctt gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga
    15721 tctcctgctt ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg
    15781 tataatccat cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg
    15841 ggttcatacg ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca
    15901 gataaaccag acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc
    15961 agccgctttc gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga
    16021 taacgaagaa catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga
    16081 ccgagtcgat accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta
    16141 aaaaccaacc gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag
    16201 cgaatgccca gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat
    16261 caaaagtagc gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg
    16321 gaggaataaa aaaaacctta caatcactgt agaaattctt ttatacagct aattgatgtg
    16381 gtcttttact cctttctata accttttgtc aactttaaca aaagtttctt cacattagtt
    16441 tacataatat caacaccatt agcatttaat gccctttccc cccagatcct tgacgactcc
    16501 aggataatta gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct
    16561 attttgacag gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg
    16621 acacgctttt gtcattccat aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc
    16681 attgtagcag gtgatttttt tcaggcgatt atttgtgcgt tcgggacgtg aatctctggt
    16741 gattgaaaaa tgaacagttt tgtacgttct gcactatgcg aggaagggtt tttactaaaa
    16801 aaaagccgct ggggtttaaa acacccccag cggctcgttt tttacactat tgtctcagga
    16861 attatctatc gtccgtcgat tactcgatat ccctttcaat caacaatgaa tcatcccctt
    16921 ccggagcaga cattgggctg tattgccacg gcttatggta gctctccatc gcctgataga
    16981 tcacctgggc cagctcatta ttactggacg ggtcatagca cagcaaatat tcggtatcag
    17041 gtagcggcgg taagccatct actccactca gcacacgcag gtccgggctc atcatttcaa
    17101 ccggccttgc cgtcacgcca agaccggctt tcactgccgc acgaacggcc ggaagcgtcg
    17161 aggcgacata agccagtcgc catggaatat ctgctttatt aagcgtcgcc agcaccatat
    17221 cgcgaaacgg acttggatca tccagcaata caagagggat cggctcgcct ttttgcaata
    17281 cgtattccgc tgcgcagtac cagtgtgttg gcgaggtacg caggttcaat gccttaaacg
    17341 ctgaaggacg atgggtggtt accatcaaat ccacttcctg ggattccagc atttctgcca
    17401 tataggcatt acgtttaacg cggacatcca gcgctaattt cggataaacc gaactcacgc
    17461 gatttaacag gaaaggtaag atcgtatcgg cagattcatc tgaagcaccg atagttaaca
    17521 cgccctgaag attactgaac attaatgatg agcaggcctc atcattaaaa cgcaggattt
    17581 tcctggcgta accaagaagt tgaatgccat gttcagttaa cagtttgttg cgaccgtgac
    17641 gagcgaacag ttctttccca acgagttgtt ccagacgctg catttgctga cttacggcgg
    17701 actgagtacg acacacagcg gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa
    17761 caaatgttct cagcagatcg aggtcgaggt taattatcgg acgatttgca cttatcatat
    17821 attatcactt actggcggct catactgagc tggttaatgc tgtgcacaca caaacaagca
    17881 attccatttg taatgtgcct ccctggcagt ttcatccgga acccggacga aagtaaaaat
    17941 gcatatgagt tgcactaaaa aagcgactca cattgttccg ttataatgcc tgaagtagat
    18001 cacagaatat atcttcaggg atcgcatatc tattaagtta ctcactcttt tctatttatg
    18061 acatgcgcgt gtttgtataa atgtaaatgt gagtccttgt tccactctcg tgcagcatcg
    18121 ctggtcatac gcgaacacgt acaaacagca atggtgaggc ggcatcaaga gcagggatcc
    18181 atcatttatg cgagcatttt accccaaaaa ccttttattt ataagggtca ttgcgaatta
    18241 tctgatgcaa agttatgtta tgttaggcaa agtaatcttc tattattaat aagcacatca
    18301 aaaccttttt gaatattaaa taataattaa ttagcatcat ccttattcat taactccgct
    18361 taacaatagt ttcacacctt gcccccatta cgacacctcg gaaatcatca cgctgttaaa
    18421 ctctccgtaa tgcggaaata tcatcatcgc gtttcattag tgaattctaa aatgaggcat
    18481 tttcacacaa tcatcttaca gataaaaaaa ccagactcac aatcaagaat cagaacaatt
    18541 cactatataa cattgcatgt aaagcatata catctcatta ttttgtcatt attaagttat
    18601 taacagcaca atcgagcctt cccctctggc aaaatcttat tctgcagacc ttcaaaacac
    18661 cctcctgggg gagtacattg ttctaagctg acttccacgg cagggagtgg cgataacagc
    18721 aaaaaaggtc aagattcatg tcccccattg aaaaatccag caaattagag aatgtctgtt
    18781 atgacatccg tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg
    18841 tactgaaact gaacatcggc aacccagccc cgttcggttt tgacgcgcca gatgaaatcc
    18901 tcgttgacgt gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc aaagggcttt
    18961 actccgcgcg taaagccatc atgcagcact accaggctcg tggcatgcgt gatgttaccg
    19021 tggaagatat ttacatcggc aatggtgtat cggagcttat cgttcaggca atgcaggcat
    19081 tgctgaacag cggggacgaa atgttggttc ctgcaccaga ttacccacta tggaccgcgg
    19141 cggtttcgct ttccagtggt aaagcggtgc attatctttg cgatgaatcc tctgactggt
    19201 tcccggacct cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg atcgttatta
    19261 tcaacccaaa taacccaacc ggcgcggtat attccaaaga gcttttaatg gagattgtgg
    19321 agattgcacg tcagcataat ctcattatct tcgccgatga aatttatgac aaaattcttt
    19381 acgacgacgc tgagcatcac tcaattgcgc cgctggcacc tgacctgctg accattacct
    19441 ttaacggact gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg atggtgttga
    19501 acgggccgaa aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc
    19561 gcctgtgtgc taacgttcct gcgcaacacg ccattcagac cgcgctgggt ggttatcaga
    19621 gcatcagtga atttattacc cctggcggtc gtctttatga gcagcgtaac cgcgcgtggg
    19681 aactgatcaa cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg ctgtatatgt
    19741 tcccgaaaat cgacgccaaa cgctttaaca ttcacgacga tcagaaaatg gtgctggatt
    19801 tcctgttgca ggaaaaagtt ctgttggtgc aagggacggc attcaactgg ccgtggccgg
    19861 atcacttccg cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct ttgagcaagt
    19921 tcgcgcgttt cctttctggt tatcatcagc tgtaatctta atttcactgc cggagattgc
    19981 atccggcagt gttatcccgc cacaatgacc tgatgatgtc atcatacgta aggtcactat
    20041 gaaacagagc catttttttg cccatctctc ccgcctgaaa ctcattaacc gctggccgct
    20101 catgcgcaac gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag cgatggtcgc
    20161 ccatgcgctg gcagctatca aaaatcggaa atttggcggt aatgtcaaca ccgaacgtat
    20221 cgctttactg gcgatgtacc acgatgcctc agaagtgctc accggcgatc tccctactcc
    20281 ggtgaaatac ttcaattcgc aaatcgctca ggagtacaag gctattgaaa aaatcgctca
    20341 gcaaaaactg gtcgatatgg ttccggaaga gctgcgggat atctttgcgc cgttaattga
    20401 cgagcatgca tatagcgatg aagaaaaatc gctggtgaaa caggcagatg cactgtgtgc
    20461 atatctgaaa tgtctggaag aactcgcggc cggaaataat gaattcttgc tggcaaaaac
    20521 gcgactggaa gcgacgcttg aatcgcgtcg cagccaggag atggactact tcatggaagt
    20581 atttgttccc agcttccatc tttcgctcga tgagattagc caggattcac cgctgtaagc
    20641 agccggagtc tgcgtcgcat caggcaataa gcgccggatg cgacatcagg ctcttgtcaa
    20701 aacggaaaca gcatcgggat catcaccaca caaaccgcca tcacgataat ggtgaacggt
    20761 acccccaact tcacaaagtc actgaagctg taatttcccg gacctaaaac cagtgtgtta
    20821 acaggtgaag aaaccggtgt cataaaggcg gcggatgctg ccatcgccac gaccatcgcg
    20881 aatggataag gcgacacacc catcgttttg gcagcagcca gcgcaatcgg agccatcaac
    20941 accgccgtcg cggtattaga gataaatagc ccaataaccg ccgacaagac aaacaaacaa
    21001 cccagcatca tatgtggccc gtaaccaccg ccaatgtcca tcagcccttt caccgccagc
    21061 gcgacacctc ccgttttctg taatgccaca gcaaacggca tcatcccaac gatcaaaata
    21121 atgctcggcc agtgaatgga tttataggcg ctttcagcat ctatacagcg gaatttcccc
    21181 atcagcaggc aggcgatgat agcggcaaca ggattaggaa tttcatctgt cagcattaac
    21241 gccaccatta gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc gggcgatgct
    21301 tcactcacct cttccggtaa gttcagcgct acgaagtcgc ggccctgttt ggccagcata
    21361 ccgatcagtt tccagttacc cacaaccagg atgatatcgc ccagcagcag aggctcatcc
    21421 gccagcgaac cttccagcgc cacgccattg cgctttagcc ccaccacatt cagtccgtag
    21481 cgggtacgaa aaccaatttc gcgcaccgat ttaccaatca gttctgactc aggaattaat
    21541 gaaatctctg ccatgcccac atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc
    21601 atcggctcca gcaattgctc actacaaaat tgccggagat cgacatcagc cgcagacata
    21661 tcaataagca aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg
    21721 cgacgaaaac gccgccagcg ttcaacaccg atgacgttag cgccataacg ctcacgtaat
    21781 ttgagatcat ccagccgttg acctatcatt ggcgatccgg ggcgaatagc cagacgtcgc
    21841 gcacgcccgg tcagtcgata ttcacggata agatcgcgaa aggttcgacg cgtccagcct
    21901 tcgcgctgcg gggtctgggt atcccctttc agcatgaaac gcatcactaa catatacaaa
    21961 atacccagca ccagcacgac caggccaata ggtgttacgc taaagaaact gaagccgtga
    22021 tagccttcac gcagcaattc actgttgact accaggttcg gcggcgtcgc caccagcgtc
    22081 atcatgccgc taatcagccc ggcaaaactt aacggcatca tcagacgtga cggcgaagtt
    22141 tgcatacgca tggcaacgct taacaccacg ggaataaaga tagcgacaac gccggttgaa
    22201 ctcataaacg cgccaagccc cgcgacggtc agcatcaaca aaaccaacat tttgatttca
    22261 ctgttgcccg ccactttgac cagccatgtt cccattacgg tggcaacacc ggtacggacc
    22321 aaaccatcgc caataataaa caaggcggca atcaggacaa cgttaggatc agaaaagccg
    22381 gaaaatactt cggggaccgt cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa
    22441 gcgaccgcat ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac ggccagtaat
    22501 gaaagaaccc aaatcaattc accgttcaca acttatcctt gttaattgag ggggatgact
    22561 tgattctgcc ataaaaaagc cccgacgaga cggggctaaa tcatgatcag gtgtttcact
    22621 gaataataac atcgccattt ggctgtttgg tcacagtaat ttgctccaga ctgtggagga
    22681 ccaaatcgac ctcattcagg cgcggggtat ctgccggagc gttaaccgca atgacatggc
    22741 aacccgccgc caagccagaa agcacgccag cgggagcatc ttccaccacc acacactcct
    22801 gcggcgcaag ccccagcagc tgcgcaccta acagatacgc atcaggttct ggttttccgc
    22861 gcttcactcg ctcagcggtt acaaacacct ctggtgcggg aagcccagct attttatggc
    22921 gcgctcgcgc taccggcatg gagccagaag tcacaatggc ccacggaata cctgctttat
    22981 tcaaatgatt gagtaaggcg attgcccccg gaagcgcggt aataccttcg gtttccgtgg
    23041 cctcgatgtg ctccagacgc gtaaactcgg cggcaatatc agcctcggat ttgcccgcca
    23101 taaaatggcg caaagaggtg atcgcctgtt taccgtgaat gaaagccagc acctcttccg
    23161 gcgctaaccc atgacgtctg gcccagttgc tccacgcccg ttctaccgca ggcagggaat
    23221 ccaccagcgt tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg gcctcctcag
    23281 gcgttgataa tttgattaat ttcgttggcg ctcaaatggt actgacgcgg gcaggcatgc
    23341 cacacattaa gcatgcgctg atatttttcc cacattggcg tctgggcgtt aaaaccgtga
    23401 gttccggcgt caaaatgggt atagcgccct tccacattaa ccataaagcg gacataaccg
    23461 aggtaacgtg cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg ttcatcgatg
    23521 gattgctggt cctgcaaatt agaccaggaa acatgcaacg catgatacat ctccataatg
    23581 tcgatgatag tgcggcaggt ttcttctttc agctcgccaa actcgcgatc caattcacgc
    23641 atctgtaatc cgtaaccacg ctcaataatt gtttgcaggc gacggtaacg ttcagcattt
    23701 gccggatcga gcatagtcat catcttgtac tgattagaca aaataagacg ttgcgcgttg
    23761 gtcatttcca ttgttgactc ctgtatcact ctactacggt gaaaaaaaga aggctgagta
    23821 tgccttcttt tatatgcgta atcaggggtc aattacaaat catcaaggaa agttttatcc
    23881 agttgtttga aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg cttgccttca
    23941 acgggtgcca acacctgtcc agactcctgc aatttaccgc gaacttcata aaaccagttg
    24001 aggatagcag ggggtaatgg cgttacagaa cgcttgccca gccaccataa tccctgcatg
    24061 ggtaaactta aggcgaacag cgcagtggca actgccggcc caagctgacc gcccagggca
    24121 atctgccagc agagagtaaa tacggcgatc ggcggcataa aacggatcgc ataacgcgtc
    24181 atcttgataa cgcgattttc gacaaagacc ggggcaaggc gtttttccag cggccacgtc
    24241 tttgagtaat gctgtccccg gcgaaacaag ctaaaaaaat taacagaacg attatccggc
    24301 gttgacatgc ttcacctcaa cttcacatat aaagattcaa aaatttgtgc aaattcacaa
    24361 ctcagcggga caacgttcaa aacattttgt cttccatacc cactatcagg tatcctttag
    24421 cagcctgaag gcctaagtag tacatattca ttgagtcgtc aaattcatat acattatgcc
    24481 attggctgaa aattacgcaa aatggcatag actcaagata tttcttccat catgcaaaaa
    24541 aaatttgcag tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct acgctctatg
    24601 gctccctgac gtttttttag ccacgtatca attataggta cttccatgtc gagtaagtta
    24661 gtactggttc tgaactgcgg tagttcttca ctgaaatttg ccatcatcga tgcagtaaat
    24721 ggtgaagagt acctttctgg tttagccgaa tgtttccacc tgcccgaagc acgtatcaaa
    24781 tggaaaatgg acggcaataa acaggaagcg gctttaggtg caggcgccgc tcacagcgaa
    24841 gcgctcaact ttatcgttaa tactattctg gcacaaaaac cagaactgtc tgcgcagctg
    24901 actgctatcg gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc
    24961 gatgagtctg ttattcaggg tatcaaagat gcagcttctt ttgcaccgct gcacaacccg
    25021 gctcacctga tcggtatcga agaagctctg aaatctttcc cacagctgaa agacaaaaac
    25081 gttgctgtat ttgacaccgc gttccaccag actatgccgg aagagtctta cctctacgcc
    25141 ctgccttaca acctgtacaa agagcacggt atccgtcgtt acggcgcgca cggcaccagc
    25201 cacttctatg taacccagga agcggcaaaa atgctgaaca aaccggtaga agaactgaac
    25261 atcatcacct gccacctggg caacggtggt tccgtttctg ctatccgcaa cggtaaatgc
    25321 gttgacacct ctatgggcct gaccccgctg gaaggtctgg tcatgggtac ccgttctggt
    25381 gatatcgatc cggcgatcat cttccacctg cacgacaccc tgggcatgag cgttgacgca
    25441 atcaacaaac tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt gaccagcgac
    25501 tgccgctatg ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt
    25561 tactgccacc gcctggcgaa atacatcggt gcctacactg cgctgatgga tggtcgtctg
    25621 gacgctgttg tattcactgg tggtatcggt gaaaatgccg cgatggttcg tgaactgtct
    25681 ctgggcaaac tgggcgtgct gggctttgaa gttgatcatg aacgcaacct ggctgcacgt
    25741 ttcggcaaat ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc
    25801 aacgaagaac tggttatcgc gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc
    25861 agctcagctg gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa
    25921 ccgtgtcccg tattattatg ctgatcccta ccggaaccag cgtcggtctg accagcgtca
    25981 gccttggcgt gatccgtgca atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta
    26041 tcgctcagcc gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga
    26101 actcttccac cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt
    26161 ccagcaatca gaaagatgtg ctgatggaag agatcgtcgc aaactaccac gctaacacca
    26221 aagacgctga agtcgttctg gttgaaggtc tggtcccgac acgtaagcac cagtttgccc
    26281 agtctctgaa ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc
    26341 agggcactga caccccggaa cagctgaaag agcgtatcga actgacccgc aacagcttcg
    26401 gcggtgccaa aaacaccaac atcaccggcg ttatcgttaa caaactgaac gcaccggttg
    26461 atgaacaggg tcgtactcgc ccggatctgt ccgagatttt cgacgactct tccaaagcta
    26521 aagtaaacaa tgttgatccg gcgaagctgc aagaatccag cccgctgccg gttctcggcg
    26581 ctgtgccgtg gagctttgac ctgatcgcga ctcgtgcgat cgatatggct cgccacctga
    26641 atgcgaccat catcaacgaa ggcgacatca atactcgccg cgttaaatcc gtcactttct
    26701 gcgcacgcag cattccgcac atgctggagc acttccgtgc cggttctctg ctggtgactt
    26761 ccgcagaccg tcctgacgtg ctggtggccg cttgcctggc agccatgaac ggcgtagaaa
    26821 tcggtgccct gctgctgact ggcggctacg aaatggacgc gcgcatttct aaactgtgcg
    26881 aacgtgcttt cgctaccggc ctgccggtat ttatggtgaa caccaacacc tggcagacct
    26941 ctctgagcct gcagagcttc aacctggaag ttccggttga cgatcacgaa cgtatcgaga
    27001 aagttcagga atacgttgct aactacatca acgctgactg gatcgaatct ctgactgcca
    27061 cttctgagcg cagccgtcgt ctgtctccgc ctgcgttccg ttatcagctg actgaacttg
    27121 cgcgcaaagc gggcaaacgt atcgtactgc cggaaggtga cgaaccgcgt accgttaaag
    27181 cagccgctat ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt aatccggcag
    27241 agatcaaccg tgttgcagcg tctcagggtg tagaactggg tgcagggatt gaaatcgttg
    27301 atccagaagt ggttcgcgaa agctatgttg gtcgtctggt cgaactgcgt aagaacaaag
    27361 gcatgaccga aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga
    27421 tgctggaaca ggatgaagtt gatggtctgg tttccggtgc tgttcacact accgcaaaca
    27481 ccatccgtcc gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg
    27541 tgttcttcat gctgctgccg gaacaggttt acgtttacgg tgactgtgcg atcaacccgg
    27601 atccgaccgc tgaacagctg gcagaaatcg cgattcagtc cgctgattcc gctgcggcct
    27661 tcggtatcga accgcgcgtt gctatgctct cctactccac cggtacttct ggtgcaggta
    27721 gcgacgtaga aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt cctgacctga
    27781 tgatcgacgg tccgctgcag tacgacgctg cggtaatggc tgacgttgcg aaatccaaag
    27841 cgccgaactc tccggttgca ggtcgcgcta ccgtgttcat cttcccggat ctgaacaccg
    27901 gtaacaccac ctacaaagcg gtacagcgtt ctgccgacct gatctccatc gggccgatgc
    27961 tgcagggtat gcgcaagccg gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg
    28021 tctacaccat cgcgctgact gcgattcagt ctgcacagca gcagtaatct cgtcatcatc
    28081 cgcagctttg cgctgcggat atctgaaccg gaaataatca ctatttccgg ttttttattc
    28141 tcttaatctg cattaatcct ttctgattat cttgcttaac tgcgctgcat caatgaattg
    28201 cgccatttca ctttgcatac ttaccacttt gttttgtgca agggaatatt tgcgctatgt
    28261 ccgcaatcac tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg ttggtgatta
    28321 tcttttttgt tgctatttta accagccttg ccacctgggt agttccggtg gggatgtttg
    28381 acagtcagga agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc
    28441 cacactcatt tcgcattctg actaacgaag caggcgaacc tgagtatcac cgcgtacagc
    28501 tgttcacgac gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa ggattaacct
    28561 caggatcgaa atacgggaca gctgttggca tcatcatgtt tatgctggtg attggcggcg
    28621 cgtttggcat tgtgatgcgt acaggaacca ttgataacgg tatcctggcg cttattcgcc
    28681 atacccgcgg gaatgaaatt ctctttattc ctgcgctgtt tattctgttt tcacttggcg
    28741 gcgcggtatt tggtatggga gaagaggccg tcgcctttgc cattatcatc gcaccgctaa
    28801 tggtccggct gggctatgac agtattacca ccgtcctggt gacctatatt gccacgcaaa
    28861 tcggttttgc cagttcgtgg atgaacccgt tttgtgtggt cgttgctcag gggattgccg
    28921 gcgttccggt gctttctggc tccgggttgc gcatcgtggt gtgggttatc gccactctga
    28981 ttggcctgat ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac
    29041 gcgtgcatga gtccgaccgc ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt
    29101 ttacctttgg tgactggctg gtattgattg tcctgaccgc cgtaatggtc tgggtgattt
    29161 ggggcgtgat cgttaatgcc tggtttattc cagaaattgc cagccagttc ttcaccatgg
    29221 gtctggtaat tggcatcatc ggcgttgttt tccgccttaa cggcatgacg gttaatacca
    29281 tggcttcatc ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg ctggtgggtt
    29341 tcgccaaagg gattttgctg ctggtcggta atggtgaagc gggtgatgcc agcgtgttaa
    29401 ataccatcct caacagcatt gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct
    29461 ggtttatgtt gctcttccag gcggtattta atttcttcgt gacgtccggt tctggtcagg
    29521 cggcgttaac catgccgtta ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg
    29581 ttaccgtgct ggcattccag tttggtgatg gcttcagtca catcatttac ccaacctcag
    29641 cttcgttaat ggcaactctc ggtgtgtgca gggtggactt ccgtaactgg ctgaaggtgg
    29701 gcgcgaccct acttggactg ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt
    29761 tgatgggcta ccactaaaaa tgttaagagc cgcaattgcg gctctttttt cattctgccg
    29821 tttcagtctc tacggcttca tttttggcat tgcgcttcat ccacaacgcc agcgctttca
    29881 gcgagtctgg agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa
    29941 cttcactgac ttcatcttcc tgtagggcga agggaccgtg agagacgcag ctgaacaacg
    30001 cgccccagac acggcaattt ttatcttcga aatagaactg cccgtgctcg gcaaagggga
    30061 caccggcaat gcccaactct tcttccgctt cgcgacgcgc ggattccagc agttgctcat
    30121 cggcctgaac cacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct
    30181 cggtacgacg ctggaccaga attttgccca tgccatcatg cacgacgatg taagttgcac
    30241 gatgacgcag acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg acttcgttct
    30301 cttcattgac aatatccacc cattcagtac ttgccaaacg acgctgttcc attatcagga
    30361 aaccttcttt ttctggcgct cttacggcgc attttgagtt gtgggtaaat tacggattaa
    30421 tcgcgacctg cgcaatgata ctttgatcat tgagtgcgat tacactaagt acgtcattat
    30481 ccagcatgcc ataactcgcc ggattaccgc ctttcggaat actcaccgag ccggggttga
    30541 agtggaaaat ctccccccgc tgttctgcca ctggcagatg ggtatgaccg tacaccagca
    30601 catcgttctg gtttaaagca ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa
    30661 acagacgttg tttttccagt aatacctgtt gccacggcgc ggttatcggg aaatgcagca
    30721 gcatttgatc cacttcgctg tcgcagttgc cgcgcacagc gataacctta tgtgccactt
    30781 cattaagccg ttcagcgact ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc
    30841 cgtgattcaa cacgtcgcca aggatcacca gccactgggc accgctttgg gcaaacaact
    30901 ccagaacacg ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac atcagtttca
    30961 tcactcactc ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg ttgctatcag
    31021 ccagattgct tcgccgaaag tgcaataaaa cgctggacag acgctcgctg ccactggaat
    31081 gtcgcataat ccaccaggcg ttctggcacc tcatcgccat gtagtaccag gcggttaatc
    31141 attagcgcca gatcagtatc agcaatgcac cattcaccaa ataaattcgg ctgacccagt
    31201 gctaacaaat gttctgccat cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt
    31261 agtggcgctt ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc gcggatgggc
    31321 atcagatcgc tgcgcagcca ggcctgaatc tgtcgtgcac gcgcacgatt ttctaaatca
    31381 agcggataaa tacgttccca ggtcggtggc gcaaatcgat cttccagata ctccgcaatg
    31441 gcagaagatt cactcaactc aaaatcatcg atttgtaata acggcacacg gcgtgtctga
    31501 ccgtaacctt gccacgtcgg ctgcaaatgt tcaccgctgt cgaggtcgat ggtcttgata
    31561 tgaaacgaca ggcctttttc ctgcaacgcc acccaggcgg ataacacata aggggagaaa
    31621 cagtgggcat ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc ggcaataaag
    31681 ggggctggct atcaaactat agagcctttt tgtcactgtc acgtgacgaa tactcacgta
    31741 agtcgccgcg ctttctatac ttgtggtgtt cagatgatta aacaaccgga gctgcaatga
    31801 tcgatctcta tttcgccccg acacccaatg gtcacaaaat tacgctgttt ctcgaagaag
    31861 cagagcttga ttatcgcttg attaaggtag acctggggaa aggcggtcag tttcgtccgg
    31921 aatttttgcg catttcgccc aacaacaaaa ttccggcaat tgttgatcat tctcctgccg
    31981 atggcggcga accgttaagc ctctttgagt ctggtgccat tttgttgtat ctggctgaga
    32041 aaacaggact ctttttgagt catgaaacgc gtgagcgcgc cgccacatta cagtggttat
    32101 tctggcaggt aggcggactg gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag
    32161 ccccccaaac cattccttac gctattgaac gttatcaggt tgaaactcag cgtctttacc
    32221 atgtactgaa caagcggctg gaaaactcgc cctggctggg aggcgagaac tacagcattg
    32281 cggatattgc ctgctggccg tgggttaatg cctggactcg ccagcgaatt gacctcgcaa
    32341 tgtatccggc agtcaagaac tggcatgagc ggatccgttc gcgccctgcc accgggcagg
    32401 cactgctaaa agcacaactc ggtgatgagc gttcggatag ttaacagaaa caggttctcg
    32461 tgtattattt catcctaagt aaaacaacgg agaacctgca atggcacaac ctgccgctat
    32521 tattcgtata aagaaccttc gtttgcgtac gtttatcgga attaaggaag aagaaattaa
    32581 caaccgtcag gatattgtta tcaatgtgac gatccactac cccgccgata aagcgcgcac
    32641 tagcgaagat atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca ttattcagca
    32701 tgtagagaat aaccgtttct ctttgctgga aaaattaact caggatgtgc tcgatatcgc
    32761 acgtgaacat cactgggtga cgtatgctga agtggagatc gataaactgc acgcgctgcg
    32821 ctacgccgat tcggtatcca tgaccttaag ctggcagcgt taatcgccat atcgggaggc
    32881 tgcatgaaca tagtgatcac cggagggacg ggattaattg gtcgccattt gattccgcgt
    32941 ttgctggagc tgggccatca aattacggta gtgacgcgta acccgcagaa agccagttcc
    33001 gttctcggcc ctcgggtgac actatggcaa gggcttgccg atcaaagcaa cctcaacggc
    33061 gttgatgcgg taatcaacct ggccggagaa ccgattgctg ataaacgctg gactcacgag
    33121 caaaaagagc gtctctgcca aagccgctgg aatatcacgc aaaaactggt cgatttgatt
    33181 aatgccagcg acacgccacc gtcggtactc atttccggct cggcaacggg ctattatggt
    33241 gacttaggtg aagtggtggt taccgaagag gaaccgccgc ataacgaatt tacccataaa
    33301 ctctgcgccc gctgggaaga aattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc
    33361 ctgctgcgta ccggtgtagt gctggcaccg gatggcggta ttctcggtaa aatgctgccg
    33421 ccgtttcgtc ttggcctggg cgggccgatt ggttccggtc ggcagtatct ggcctggatt
    33481 catattgatg atatggtcaa cggcattctc tggctgctgg ataacgacct gcgcgggcca
    33541 tttaatatgg tttcgcccta cccggtacgc aatgaacaat ttgcccatgc gctcggtcaa
    33601 gcgctgcatc gcccggccat tttgcgcgtc cccgcaaccg ccattcggct gttaatgggc
    33661 gaatcttcaa tactggtatt aggtggacaa cgcgcgctgc ctaaacggct ggaagacgcg
    33721 ggttttgcgt ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt
    33781 ggtttacagc aaacatccgc cagttaactc ccggtgttac aggattagtg gctttgcgcg
    33841 ataagatcgt ctggtgaaag tcgggtcacc atcataacta actctctgtc taaacctcta
    33901 tccagcatct cctgagcaat acgcagggct tcttcgtgtt tgccctgcat tgcgccttct
    33961 tcacgtaatc tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt
    34021 atctcaccaa taaatgcacg gaaacgctgg gcatcccctg tttgtaatac gtaattaaac
    34081 agggctttta gctgtctgtc attagtgttc cctgtaacta gcagcgaaac aatttggtcg
    34141 actaatccca acagatcgcg ctgacgaata tgtttctgaa ttaactccaa cagcgccatt
    34201 ttgcggtgtt gcataatctc gtcatccggc accacggtaa tatccaccaa cggaaacgct
    34261 gatgaatata ttttgcgggc tatagcaggc tcggcaaatt catcaagcca gcagagtgaa
    34321 taaggataag gacttctgca accatgataa aacagcatcg ggatcaccaa tggaagctct
    34381 ttatagcccg catcaagatg gttttgcatt gccgcaatgg aataacgcat catgcgaaaa
    34441 gccattaatt cttccggctt actttggtgc tctatcacta cataaatata acccactccc
    34501 tcctgcgttt tcacagacca caagaggtcg gaataatatt gccgcaggtc ttcatcaata
    34561 aaactgtttg gttctagttt aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga
    34621 agatgaatat caataaaatc ccgcgcggtg tctggatggc gtaaaaaaga tttaaatacc
    34681 gcatcatgcg gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc ctcaaattta
    34741 agatgacgcg acaataacca cgattaacac ccaatgcagc aggcagtttt tatctttgga
    34801 cagcgaattc agaagtattt ttagcggact gcaactttta catttcagcg gaagctgact
    34861 cgcaaattcc ccatgaaaat actccggcag cgttagccac cggagaggaa attatttcag
    34921 cgatccctta aggaactgtt gcagacgagg gctttgcggg ttgccaaata actgctccgg
    34981 cgcgccctct tcttctattt tcccctgatg gaggaaaatg acgtgagtag aaacatggcg
    35041 agcaaagccc atttcgtgag tcactaccac catggttttc ccctcttctg ccagttgctg
    35101 cataatacgt aacacttcgc ctaccagttc aggatcgagc gccgaggtag gttcatcaaa
    35161 cagtaaaact tccggttcca tcgccagcgc ccgcgcgata gaaacacgct gttgctgacc
    35221 gccggaaaga tgcaccggat atttcccctg cgcacgttcg tctatcccga ctttcgccag
    35281 atacttcacc gcccgctcgc gcgcttcctg cttgctcagg cccaacacct gaatcggcgc
    35341 ttccatgacg ttttccagca ccgtcatatg gctccagaga ttgaagtgct ggaataccat
    35401 cgtcaggcgt gtgcgcagta agcgcagttg atttttatcg gcgactttga gttgaccgtc
    35461 tttgtcgcgc accagattga tcgtctggcc attgaccacg atcgaccctt cactcggttt
    35521 ttcgaggaag ttaatgcagc gcagaaaggt acttttcccc gatcccgacg atccgatgat
    35581 gcttattaca tctccggcat tcgcttgcag tgagacccct ttcagcactt catgttcgcc
    35641 gtagcgtttg tgcaaatcga taacgtttaa tttattctcg gacatcgtat tctcagtgcg
    35701 ttgaagaagg tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag aggctgatca
    35761 ggacataaga gatgattaaa tagagcaccg cggcaatgcc gaaggcggta aaaggttgat
    35821 acgtggcggc gttaatatcg cgggctattt tcagcagatc cggcacagtg gccgtaaacg
    35881 ccagcgcagt agagtgcagc atcaggatca cttcgttgct gtatgccggt aacgcaatac
    35941 gcagcgcaga aggcaaaata atgcaacgat acattttaaa agtcgagaag ccataggctc
    36001 tggcggcttc aatttcccca tgcggaaccg aacggattgc cccagcaaaa atctcggtag
    36061 tgtaagcgca ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca ctgcggaaga
    36121 aagcgttaag gaattcggtt cccttaacaa tctcaagcgt gtacatgccg gaatagaaca
    36181 ccagcaactg aacatacagc ggcgtaccgc gaaaaatata ggtaaataac cagattggaa
    36241 actggatgta tttattactg gagacacgac caatcgccag aaacagcgcc aggactccgc
    36301 ctatcactac cgacaaaata agcagccaca gagtgatcgc cacaccagta aagcgataac
    36361 cgtcggtcca cagcagcggt ttccagtatt catgtaagat ttcgatcaca ggtcagccct
    36421 cttcacaccc acggagtagc ggcgctcaag gaacagcagc acaccattgg aaacagtggt
    36481 gaaaaccagg taaatcacgc cacagacgat ggcgaaatag aacggttccc aggtactttt
    36541 gcctgccagt tgggtggctt tgaccacatc ttccaggccg agtaacgaaa ccagtgcggt
    36601 agatttgagg atcacctgcc agttgttgcc aatgcctggc agggcgtaac gcatcatcgc
    36661 cggaaacatg atccgccgaa acacttgccc acgagtaaaa ccgaacgccg tcgccgcctc
    36721 tatatgtcct ttcggcactg ccataaaagc accacggaac gtttcggtaa aataagcacc
    36781 gtagataaaa ccgagagtga taataccagc gaccatcgga tcgatatcaa tctgcccgac
    36841 gcccatcgcc tccgtcaccg tgtttagcgc aatctgcaaa ccgtagaaaa tcagcagcat
    36901 caacactaag tccggcacgc cacgaatcag cgtggtgtac ccttcgaaaa tcagccccga
    36961 aagccgattt tgcgagagct taccgccagc gccaattaaa ccgatgatta cagcgagcac
    37021 tacagagctg atagccagct ccagcgtgac gagcgcaccc tgtaaaataa cacctgaaaa
    37081 cccatacaac atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct tgtctccctc
    37141 cggttacccg aagggagcgg gctttctttc accgatggcg attagccacc ataaacatca
    37201 aaatcgaagt actttttcgc taatttctcg taagtaccgt cagcgcgcat ttcggcaaag
    37261 gctttgttca gtgcttcgcg cagttcgtta tcttctttac gcaggcccat gccggtccct
    37321 acgccaaaca gtttttcatc tttaacagac gggccaccga atttgtaatc tttaccgaca
    37381 ggttgtttga ggaaaccttc gctggcagcg acctcatctt ggaacgcggc atcaatacgt
    37441 ccggcagtca ggtcagaata aatgttgtcc tgcccctgat acgagacgat ttcaatgcct
    37501 tttggtgccc aatgttcatt accgaacgtc tcctgggtgg tgccctgcaa tacgcctacc
    37561 cgtttgcctt tcagcgactc gactgtcggc tgaatgtcag aatttttcgc caccaccaaa
    37621 cgagaatcgg cagcgtacag tttgtcggtg aaggctattt cttgctgacg tttttccgta
    37681 atggaaagcg atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg gattaacgca
    37741 tccagcggat tttcgacaaa cgtacattgc gtattgatgc gtttgcataa ttcctttgcc
    37801 agatcgatat cgaagccaac cagttcgcct tgtgaatttt ttgattcaaa tggcgcatag
    37861 gtcgggtcgg taccgatgcg gatgttttgc ggaatcgcag caaacgccgc agttgcgctg
    37921 gagaaggcca gaaccagaga gagcgatagc accagttttt tcataatgtc ctcaactgac
    37981 agtcttttta tagggaatat ttacaggttt gttacactta tcgtgccata aaattgacca
    38041 tcaaggcaaa atatcccgcc ccgttagacg tattattttt cattaaaaat gcttaagtat
    38101 gcatttcaat gcaccacgat agtgcaacaa acctggcgtg caccatgatg atcattccat
    38161 caggtacagc ttcccagcga cgtatcagtc accgtagaca ttaaagtcga aatacttttt
    38221 cgccatcttg tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat tgaaggcagc
    38281 cgtcatttca gcatcatctt tacgtagccc tacaccggtg ccatcaccga agtatttttt
    38341 gtcttttact gatgagccag caaaggcgaa atctttacca gcaggttgct tgaggaatcc
    38401 ttcgctggca gcaacttcat cttgtaacgc agcatccaga cgtcctgcag ccagatcgga
    38461 atagaccaaa tcctggttgg cataggccac cacatccacg cctttgctac gccaggtctc
    38521 gttagcgtaa gcttcctggg ttgatccctg cagcacacca acatgtttac ctttcagtga
    38581 atccagcgtt ggctgaatcg gtgaaccttt ggcagcaatc aaacgagaat ctgcggcgta
    38641 cagcttgtcg gagaaggcaa tctcctgctg acgtttatcg gtaatggaaa gcgacgaaat
    38701 aatggcgtcg atttttttcg ctttcagtga ggggatcagc gcgtcaaagt cactggcaac
    38761 ccaggtacat ttcacctgca tccgtttgca catctcgtta ccgagatcga tatcaaagcc
    38821 aacaaaatca cctttagcat ctttcgacga gaacggcgca taggtggtat cggttccgat
    38881 acgtaccgtc tccggtagcg ccgcatagct ggaagccgct gcggagagac cgactaacaa
    38941 agacagagcg agaatcgact tcttcataca taaccctcaa gtgaaaatgg cttttttatg
    39001 ttgtgtattg tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc atcttgacga
    39061 caacagtaac attcaacgtt aaatatgtta ataagacgtt gcattattgt cctgaagttg
    39121 aagatagcag gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga aacaaaatgt
    39181 cgaggatttg atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg agttatgcgc
    39241 cctgccagcg ggcaaagaga tcttcaggaa gggttattgc aaactggtca agaacacgat
    39301 taaccgtctg atttatcaca tcatcaaggg attgcgggcg atgataaaac gccggaacgg
    39361 gaggcataat caccgcaccg atttctgccg cctgagtcat taaacgcaga tggcctaagt
    39421 gcaatggtgt ttcacgcacg cagagcacca acgggcgacg ctctttcagc accacatctg
    39481 ccgcacgggt cagtaagcca tcagtatagc tatggacaat gccggaaagg gttttgattg
    39541 aacagggtaa aatcaccatc cccagcgtct ggaaagaacc ggaagagatg ctggcggaaa
    39601 tatcgcgcgc atcgtgcgtg acatcagcta atgcctgcac ttcgcgcagg gaaaaatccg
    39661 tttcgaggga taaggtctgg cgcgccgcct ggctcatcac cagatgcgtt tcgatatctg
    39721 tgacatcgcg cagaacctgt aataagcgca cgccataaat cgcgccgctg gcaccgctga
    39781 tgcctacaat gagtcgtttc ataattagtt gccctgtcag acttgcaggc agactttgca
    39841 gtatttcggc gggagttgca agtcagggtg ccagaccggc accctcagcg aaggcatcat
    39901 ccttcgttat gcatttcgag attttccact tcgttctgac gttgcactgc tttggcgtca
    39961 tcattacgta acgtatcgag gaaatcgagg tagccctgat caacatcttt ggtgacgtag
    40021 acgccgttga acaccgagca ttcaaactgc tggatatccg gattttcagc gcgaacggcg
    40081 tcgatcagat cgttcagatc ctggaaaatc aacccgtcag caccgatgat ctggcgaatt
    40141 tcatcaactt cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc aataccataa
    40201 acgttcggga agcgaatttc cggtgccgca gaagcgaggt acactttctt cgctccggct
    40261 tcgcgtgcca tctcgataat ctgctcagaa gtggtgccac ggacgatgga gtcgtcgacc
    40321 agcagaacgt ttttatcgcg gaactcggcg cggttggcat tcagtttacg gcgcacggac
    40381 ttacgacgca gctgctggcc cggcatgata aaggtgcggc caacatagcg gtttttaacg
    40441 aagccctggc ggtacggttt gcccagaata cgagcaattt ccagcgcgat atcacacgag
    40501 gtttctggga tcgggatcac cacgtcgata tccagatctt cccattcgcg ggcaattttc
    40561 tcgcccagtt tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt gtcgatgaac
    40621 gagtccgggc gagcaaagta tacatactca aacaggcacg gattgctgac cggattgtca
    40681 gcacattgac gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc gcccggcgcg
    40741 acgtcacgca ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga agcgaccata
    40801 tattctgtac ggttctcgtc aatatcacgt tttcccagta ccagcggacg aatcccgttt
    40861 ggatcgcgga aagcaaccat accgtggccg ataatcatcg ccacacaggc atacgcgccg
    40921 cggattaagc ggtttgtggc agcaatggca gcgaaaatat tgtcggcttc cagcgggtag
    40981 tggcggaaat tgtccagctc gctggcgaag atattaagca gaatttccga gtcggaagtg
    41041 gtgttgatgt ggcggcgttt ttcttcaaac agttttttac gcaactcgtg agcgttggtc
    41101 agattgccgt tgtgggcaag cgtaatgcca tacggggagt taacgtaaaa cggctgcgct
    41161 tcagaggcgc tggagctgcc agccgtaggg taacgcacat gaccaatgcc catattgccc
    41221 tgcaaacgct gcatatggcg agcttcaaat acatcgctca ccagcccgtt cgctttacgc
    41281 aaacggaagc agttattggc atctatggtg atgatgccgg cggcatcctg accgcgatgc
    41341 tgaagcaccg ttaaggcatc ataaatcgac tggttaaccg gcataacacc ggcgataccg
    41401 acaataccgc acatacgtct ttttcctcgt taagccacat ctcagagcac ttacgctctg
    41461 ggcaagaaac ttgacgagct ttgcagataa tcaaaaaacc atctgatgat aaaactgaat
    41521 tgcgggatca gctgtgattt gctccagtct tcgcttttcg acaccccggt aaaggagtcg
    41581 agaaagaaga gaatggcggc aacaatcaac acaccgcgca acgcaccgaa acagacgccc
    41641 agcacccgat cggtgcctga caaccccgtt ttctccacca actggcctat cacgaagttc
    41701 acgatagcac caacgatcag ggtagcgata aacagtaccg cgatggcaat cccatttcga
    41761 accagttcgt cttcaaagcc cgtaaaccag actgacaggt aagtgtagta atgactggca
    41821 acaaagaaag cacaacccca tgtcaccagc gataacgctt cacgaacaaa gccgcggatc
    41881 aggctaacca gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat ccagaccata
    41941 tgtgtcccac gattttacgc cctgtcatcc tgttcggggc gcattctaac agaaaaagaa
    42001 aacgtttgcg tagggatttc cttcccacgc atcaataaaa atggcgctga aaaaatattc
    42061 aacgccatcg actttttatg cctttgcggc atcgtgcaat gcgtgtcgga tgcggcgtaa
    42121 acgccttatc cgacctacgg ttctaacact gcccaggcct gataagacgc gccagcgtcg
    42181 catcaggcaa gactgtatta attcggcgta tagcccatta ccacgccact taagccagaa
    42241 agttgcttca actcacccag cgaacctttc agcttatctt tcgaggcatc cggcccaacc
    42301 agaatacggg taattttacc ctgcactggc gtggatggcg acgtataaac ccgataaccg
    42361 gcaccgcgca gcttaccgac aatctcattc actttatcgg catttttcag cgcacccagt
    42421 tgcacaacat aggctttacc cgttggcgct gctttttctt ccacgaccgg cttcggttct
    42481 ggcttcggcg caggtggtgc ttcaaccttc ggtttaggcg gctccaccgg tttcggcttc
    42541 ggtggggcga ccggtgcagg ttccggttca aactcggtgt tattggctgc aatagtggcc
    42601 ggatcgagcg acggtgctgc ggcatcacct gcccgcacct cttccgctgc gccttccggc
    42661 ggctgcgtcg gcaacgcctg ggtggcggct ggcatcatat caggctcatc acgatcgccc
    42721 gctttcggca ccagcgggat agccgcaaac tcatcctgat aatgtttttt ctgcccgtcc
    42781 agcagccctg gaagtacaat cacccccagc gccaccagca cgatcgtgcc cactaaccga
    42841 ttctgaaact tacttgccac cgcttctcct cgcgtcaatc acttccatga catgtgcgac
    42901 cgtgtggaaa gaaccacaca ccagcacggt gtcttccgct ttagcgtccg ccattgcggc
    42961 atcccatgcc tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct caagcagttg
    43021 ttctgccgtg gcaccgcgcg gcccttccag tggcgcacaa taccagtcat caaccacgct
    43081 tttcaaccag gccagagttc cggcaatatc tttatcatgt agcataccga taaccgccag
    43141 cacgcgcccg tttttcggta gcgctttcat acgcccggtg agatattccg ccgcatgtgg
    43201 attatgcgcg acatcaaaaa taacgcgtgg cgactcgctc acaatctgga aacgtcccgg
    43261 caaaattgcg ctggcaatcc cgtcgcgaat ggcattttca ctgacctcca gcccgctggc
    43321 acgcagtgcc gccagcgccg ttgcggcatt cggttgcggg acaagcggca acggcaggtt
    43381 ttccagcgtg ccgtgagcat cgctaaacgc ccagtcatga tcggtgacgg aatagttcca
    43441 ctcaacgccc cgacgttgta acagtgcacc tttttcctgc gccacatcag caatggtaga
    43501 aggcatttcc ggctcaccga caattgccgg ttttgcgctg cgaaagatgc ctgctttctc
    43561 gcgaccaata ctttcgcgat ctggtcccag ccagtcggta tgatccagcg caatgctggt
    43621 tactacagcc acatcggcat cgacaatatt ggttgcgtcc agacgaccgc ccagccctac
    43681 ttccagaatc actacgtcaa gttgtgcctg tttgaacagc cacaacgccg acagcgtacc
    43741 gtactcgaaa taggtcaggg aaatatcacc gcgtgccgat tcaatctccg caaaagaggc
    43801 ggtgtgggcc gattccggca attcctgccc ctgcacacgt acgcgctcgg tataacgcac
    43861 cagatgcggt gaactgtaga cgcccacttt gtaccctgcc gccatcagaa tcgactccag
    43921 cgtacggcag gtggtgcctt tgccattcgt acccgcaacg gtaaacacaa atggcgctgg
    43981 tttctggacg ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt
    44041 tttactgtgc aggttttcca gataagaaag ccacgaagcc agaggcgacg cggcttgagg
    44101 agtgcgtttg ataatcatgg tatccgctga ttcgttacgg tgagaatagc aaaagggcag
    44161 agccagtggc cctgccctta tcagttatca ggcctcaggt tcctgatccg gtaccggggg
    44221 taccactacg ccttcacgcg gcgcttcagg attcggcgct ggcagattca tcaacttcgc
    44281 cagaatgctc gccagtttca ggcgcatttc cggacgacgg acgatcatgt cgatggcgcc
    44341 tttctcgatc aggaattcac tgcgctggaa tccaggcggc agtttttcgc gaacggtctg
    44401 ttcgataaca cgcggaccgg caaagccgat taacgcttta ggttcagcga tgttgagatc
    44461 gcccagcatg gcgaaacttg cagaaacacc gcccatcgtc gggtcggtca gcacggagat
    44521 gtacggcaag ccgcgctcct gcatttttgc cagtgccgca gaggttttcg ccatctgcat
    44581 cagcgacatc agtgcttcct gcatacgtgc gccaccagag gcggagaagc agatcagtgg
    44641 gcagttatct tccagcgcct gctcaacggc acgcacgaaa cgtgcaccca caacagagcc
    44701 cattgaaccg cccataaagg cgaactcgaa tgccgcagcg acaaccggca ttccatacag
    44761 agtgcctttc atcaccacca gcgcatcttt ttcgccggtt tctttctgcg cagatgccag
    44821 acggtcttta tacttcttgg agtcacgaaa cttcagcaca tctttcggct caagctcgct
    44881 acccagctcc acaaggcttc cttcatctaa caggctatgc aggcgattac gcgctgtcat
    44941 acgcatgtga tggtcacact tcggacagac ctcaagatta cgttccagct cagcgcggta
    45001 taaaacctga ccgcagctat cacacttagt ccacacccct tcaggaatgc tcgccttgcg
    45061 ggtgggagta atgttgcttt taattcgttc aatccagctc attagggacc tttctgtctg
    45121 aacctggttc gatgccagtt ttatctttgg ggacgcataa tgccattttt gcccccaaca
    45181 gaccatgaat gttgcacatt aaaacataac agcccgaaac tttggataaa aaagtggtcg
    45241 aaccgcggag ttacttttta ctttgcggcg cgtgccgcag cgcgtttgtg acggattatt
    45301 tcgatgacac ccggcaaaat agaaaccaca ataatcccga cgatcagcag cttaaggtta
    45361 tcctgaacca tcgggattgt accgaagaaa tagcctgcgt aggtaaaaag cagtacccac
    45421 aacagtgcgc cgatcacgtt ataagcggcg aaatgacggt acgacatgtg gcccattccc
    45481 gcaacaaacg gggcgaacgt tctgacgatc ggcacaaaac gggcgagaat aatcgttttg
    45541 ccgccatgtt tctcataaaa ctgatgggtt ttgtcgagat aactgcgacg gaaaattttc
    45601 gaattgggat tactgaacaa cttttcaccg aacaaccgcc cgatagtgta attgaccgcg
    45661 tcacccacaa tcgcggcgat cagcatcagc accaccatca tatggacgtt gagatcgttg
    45721 gtttccagtg atgccaacgc tccggcaaca aacagcaatg aatcacccgg taaaaacggc
    45781 gttaccacca gaccggtttc acagaacaaa attaagaaca aaatggcata aacccagacg
    45841 ccgtactccg cgaccagctc tgccagatgc acgtcaatgt gcaggataaa atcgatgagg
    45901 aaataaatca ggtccatatt tgcctatgcc ttgtactcgt catctttcag gctgtaacta
    45961 cgttggctac agcctgaaat gttccgagca ttattctcgt tagtccgcca gaaatagcgg
    46021 gcccattggc ggttttggaa gatcataccg gtcagggtaa tccaccgcga ccagatacag
    46081 cccttccgct tttgccgttg ctgccgccag cgttctgtcc tttgccgcca gcaactctgc
    46141 tatccagctc tccggctggt tgtgggcacc gacttccatc aggctgccga caatattcct
    46201 gaccatatga tgtacaaaag cattcgcttt aatatctacc accacataag gaccgtgacg
    46261 cgtgacatta atatgcataa cgttgcgcca cggggttcgg gactggcact gcaccgcacg
    46321 gaacgaggtg aaatcattct cgcccagcaa gcattgcgca gcccgatgca tccgttcagc
    46381 gtccagcggt tcgtaaaaat gggttacccc tttactcagt accgccgggc gcagccgatg
    46441 attgtagatg atgtagcgat aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc
    46501 aggtacagct ttaacccaac gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa
    46561 ggtccacgcc gcgtctttgc gctgcgcggt agtttcgaaa tgcaccacct gcccggtacc
    46621 gtgtaccccg gcgtcagtac gcccggcgca gaagacggtg atgggttcgt tcgccaccag
    46681 ggaaagcgcc ttttccagct tctcctgcac acttcggact tcattctgcc gttgccagcc
    46741 gtaataccta ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg gcggttgttg
    46801 ctggtcggac attagtacag atactcctgc accagtttct cggcgatttt tactgccatc
    46861 agcgcgccgc caaagcgaac gttatcggcc accgaccaga actggacttg ctccggcata
    46921 ccgtagtcat tacgcacgca gccaacagaa agatgcggcg tacccgaagc atcccctacc
    46981 tgagtcggga attcgttctc ttcagagagc acaatatctt cgccctgagc aaacgcatca
    47041 cgcgcttctt ctgccgccag cggacgcagt gcttcaaagt tgaccatctg ggcatgaccg
    47101 tagaataccg gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc ctgcaggatt
    47161 ttgcgtactt cgtcaacgat acgacgttct tcacgcacgc taccttcgct atccggcaac
    47221 aacggcagca tattgaacgc cagttgacga ccgaagaaat cttcttcgtc aatcggaatg
    47281 ccgttgagca atttcgcact ctgccccgct aacgcatcga ccgctttttt gccctgggcg
    47341 gaggctgaaa tcaggctggt aacactgata cgcgacaaac cgccctgatc gattaacggt
    47401 ttcagtgccg ccagcagctg gctggtcaga ctgtctggta cggcgatgac attccggttg
    47461 cggtaatcgg tcagtacaaa cgggtttact tccggcacca ccagcggtac gtcgggttcg
    47521 agagcaaaca gtccgctgct gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc
    47581 caggcagcgg tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg
    47641 aattcagcgg catcctgcac ggtgattgtc ttaccaccaa agcgcagttg ttcgcctgcg
    47701 ctttcgttac gtgccagtgc ataaatttcc ccaaccggga actgacgttc agccagcgtt
    47761 tcaagcaggg cttcgcccac agcgccagtt gcgcccagga cggcaatgtt ccagccttca
    47821 gacatggtgg tttactccag aaatagcaaa gctccctgcc aatgttacag cagagagcat
    47881 gaagaagaga ttaacgtgcc ggatgatgaa cggcgttaaa acccagttta cacagcaatg
    47941 atgccgcact ggcgtcatca caaattacat acagagatga ccattcacgg cgctcaagat
    48001 agtttttgcg cagtttatcg aactcacccg gtatcccggc gactttacgc agcggtgcgt
    48061 catcgcggcg cacatcatac accaaatgca ccagcctttt cagcgtcggt tgatcgagcg
    48121 gaccatgcag cgtaatgcgg ccaaactcag gcgcgggcag caacgtatcc agcgcaacgt
    48181 gttgttcatg cccaataaac ttgctatagg cttcaaacac ttgcgtcgtg ccacgcgctt
    48241 taccctccag ggtatagcct gcgatatgcg gcgtgccgat atccactttt gtcagcaact
    48301 ccacgttaag ttccggttcg ccttcccaga catccagcac tacgcttaac ttctggcctt
    48361 cattcaggca ggtcagcaac gcagtattat cgacgactgc gccacggcag gcgttaatca
    48421 gaatcgctcc cggcttcagg ctacggatca gtttttcatc cgccagatgt agcgttttgt
    48481 acggaccatc tttaaagagt ggcgtatgga aagtcagaat atccgcatgc tgaactaatt
    48541 catccagcga gcggaaatca ccctcatccc cacggtcggc gcgaggcgga tcgcaaagta
    48601 aggttttaat ccctaacgct tccagtcgtg cctgtaaacg ccgaccaacg ttaccaacgc
    48661 ccacaatccc caccgtacgg tcgtgcagtg aaaatccatc gcgttcggca agcatcagta
    48721 gtgaggagaa aacatattcc accaccgcaa tcgcattaca gccaggtgcc gcggaaaaaa
    48781 caattcccgc ctgctttaac catgcttcat cgacatggtc ggtccccgct gtggcagtac
    48841 caacaaattt aatgggtttt cctgccagca aagattcatt cactttcgtg accgaacgca
    48901 ccatcagcgc atccgcgtct gccagttgag cgacggggat tggacgcccg ggaaccgcgg
    48961 tcacctcacc caaacggcta aataagtcgc gggcataagg catattttca tcaacaagga
    49021 ttttcacgtt tgtgttacct gtatgagacg agagttaacc ggacaagtgt gccataatct
    49081 cgcggccagg catacttgcg aagatttcag gtataaggat acgtaatgat acaacctatt
    49141 tccggccctc ctcctgggca accaccaggt cagggagata acctgccgtc tggcgcgggc
    49201 aatcagcctt tatccagtca gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc
    49261 tcactgacgc aacagcaaag agcagaactg tgggcgggta tcaggcacga tattggtctg
    49321 tcgggagatt caccgctgct ttcgcgtcac ttccctgccg ctgaacacaa cctggcgcaa
    49381 cgtctgctgg ccgcgcaaaa aagccattct gcccgccagc ttttagcgca attaggggag
    49441 tatttacgtc tggggaataa tcgtcaggcg gtcacggatt atatccgtca taactttggt
    49501 cagacgccgc tgaatcagct ctcaccggag caattaaaaa ccattctcac cctgttgcag
    49561 gaagggaaga tggttattcc gcaaccgcag cagcgcgagg cgaccgaccg tcctttatta
    49621 ccggcggagc acaatgcgct taaacagctg gtgaccaaac ttgcggcggc aacgggggaa
    49681 cccagcaaac agatctggca atcgatgctg gaactttccg gggtgaaaga tggcgagtta
    49741 attccagcga aactgtttaa ccatctggtg acctggctac aggcgcgtca gacgctaagc
    49801 cagcaaaata cgccgacact ggaatcacta cagatggcgc taaaacaacc tttagatgcc
    49861 agtgaactgg cagcgttatc ggcgtatatc cagcaaaaat atggtctttc tgcgcaatca
    49921 tcgctttctt ctgcccaggc cgaggatatt cttaatcagc tttatcaacg gcgggttaaa
    49981 gggattgatc cgcgtgatat gcaaccgctg cttaatcctt ttccaccgat gatggacacg
    50041 ttgcaaaata tggcaacgcg tcccgcgctg tggatactgt tagtcgcgat tatcctgatg
    50101 ctggtctggc tggttcgtta gccccgacga aatgacagta tcgtgacaat aattcccagc
    50161 actgcagaga tcgccccggc aagaaatacc gaagagtaac caaacgtggt cgccagcatt
    50221 cccgccagcg gcccggagac gccgagggcg atatcctgaa acgcggcgta accgcccagt
    50281 gcggtgccgc gaacttgtga ggggacgcgt ttaaccacct ccacgcccag cgcaggaaag
    50341 ataagcgaac atccggctcc ggttaacgcc gcgcccgcta atgcgaccca tgcacctggg
    50401 gcttgccaga gcagcaacaa gcccaccgtt tctacaagca gagagacaat cgccactttc
    50461 acgccgccaa aacggtccgg catccagcca aacatgacgc gcatcacgac aaatgcgccg
    50521 ccaaacgcgg taagagtaaa gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa
    50581 acgaaagtcc cgataaccgc aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc
    50641 cagataagcc cgacaacgct ccacagcgat ggacgttctc ccgccagggc cggtactttg
    50701 cgcactgtgc cgttacaggc ccacgccagt aagggtaata ccattgtggt gatcgccagt
    50761 gcggcaaaac cgtaatggct atgaatcaac aggccaagcg gagcaccaac agcgagggca
    50821 ccgtaaatcg ccattccatt ccatgacatc actttgccag agtgttttgc ccctacgatg
    50881 cctaaccccc aggtcagagc gcctgtcagt aactggcttt caccaaaccc aagaatcaaa
    50941 cgcccgatga ccaacagggc aaatttgaac ggtgcggaga caggcaaaat cgccgccagc
    51001 agcaacgcgc cgccagccag accacaagct aacatcccct gaagcgccga acgttttgca
    51061 ccatattgat cggccagtcg cccggcatag ccacgcgtca gcaccgtagc cagaaactga
    51121 atcccgacgg caatgccgac catggtattg ccatagccca gttcatgatg aacaaacagc
    51181 gggataaccg gcaacggcag ccctacggtc atgtaggtga gaaaaaccgc aaaagcgatg
    51241 cggaagagcg aaaaattggc agaagatcgt gtttcggttt ggcttacagc agtcatgcat
    51301 tactccagaa tgcagcgcaa ggcgaggagt atccccgtct catctctctg gtttcagggt
    51361 tacggtgcgt tggtaggatt taacgcgtac gtcttttcag aaggaaatcg acaaagcggg
    51421 aagtttgcct ggaacaggcg gcgattgtca atgatgtgaa aaagggaacc atcaggttcc
    51481 cttttgcgtt agtgccggag gccgcattag cgcgtaacgt cggatgcgac gctggcgcgt
    51541 aacgtcggat gcgacgctgg cgcgtcttat ccgacctact ccgactactc cgacctactg
    51601 cgaattaatc tttcagcttg cgcattacca gcgtggcgtt ggtgccgccg aagccgaagc
    51661 tgttagacat aacggtggtc agttcgcgat cggtcgtttc ggtcacgatg ttcagacccg
    51721 cagcctgctc gtccagctct tcaatgttga tgctcggggc gataaagccg tgttccagca
    51781 tcagcagaga gtagatagct tcctgcacac ccgcagcgcc cagtgagtga ccggtcatgg
    51841 ctttggttgc agaaatcgcc gggctcttat cgccgaacac ttcacggata gctgccagct
    51901 ctttcacgtc gccaaccgga gtcgaagtac cgtgagagtt caggtaatcg attggggtat
    51961 caacgccatg catcgccatc tgcatacaac gtactgcgcc ttcgccagac ggagcaacca
    52021 tgtctgcacc atcagaggtt gcgccgtagc caacgatttc agcatagatg tgagcaccac
    52081 gcgccagcgc gtgttccagc tcttcaacca ctaccatacc gccgccgcca gcgataacga
    52141 aaccgtcacg gtgagcgtcg taagtacggg aggctttttc cggggtgtcg ttgtatttag
    52201 tagacagcgc acccatagcg tcgaattcgc aggccatttc ccagcacagc tcttcgccgc
    52261 cgccagcaaa cacgatgtcc tgtttgccca gttggatctg ctctactgcg ttaccgatac
    52321 agtgtgcgga agtcgcacac gcggagctga tggagtagtt aacgccatga attttaaacg
    52381 gcgtggcgag gcaggcagaa acgccggatg ccatcgcttt ggtgaccaca tacgggccaa
    52441 ccgctttcag gccgcgcggg ccgcgcattg cgtcagcgcc gaatacctgg aaacgcgggg
    52501 aaccgccgcc agaacctgca atcaggccaa cgcgcgggtt attctggtaa gcttccggag
    52561 aaaggcccgc atctgcgatt gcctgctcca tagaaaggaa tgcataaatg gatgcgtcgc
    52621 tcataaagcg cacaactttg cggtcaatga ggccagtggt atccagtttt acgttgcccc
    52681 agacgtggct acgcatgccg gaatccttca gctcctgaga gaaagtgatc cctgaacgtc
    52741 cttcacgcag agatgccagg acttcctgct ggttattacc gatgctggaa acaatgccca
    52801 ggccagtaat cactgcacgt ttcattcaat acctctgtaa gtcgcacata gagtaagttt
    52861 cgaatgcaca atagcgtaca cttgtacgcc gaacaagtcc gatcagccat ttaatagaga
    52921 aatttgcgca gccttacaca catcgctaag atcgagccac cgcctgtaag acgagtaact
    52981 tacgtgaaac actactccat acaacctgcc aacctcgaat ttaatgctga gggtacacct
    53041 gtttcccgag attttgacga tgtctatttt tccaacgata acgggctgga agagacgcgt
    53101 tatgtttttc tgggaggcaa ccaattagag gtacgctttc ctgagcatcc acatcctctg
    53161 tttgtggtag cagagagcgg cttcggcacc ggattaaact tcctgacgct atggcaggca
    53221 tttgatcagt ttcgcgaagc gcatccgcaa gcgcaattac aacgcttaca tttcattagt
    53281 tttgagaaat ttcccctcac ccgtgcggat ttagccttag cgcatcaaca ctggccggaa
    53341 ctggctccgt gggcagaaca acttcaggcg cagtggccaa tgcccttgcc cggttgccat
    53401 cgtttattgc tcgatgaagg ccgcgtgacg ctggatttat ggtttggcga tattaacgaa
    53461 ctgaccagcc aactggacga ttcgctaaat caaaaagtag atgcctggtt tctggacggc
    53521 tttgcgccag cgaaaaaccc ggatatgtgg acgcaaaatc tgtttaacgc catggcaagg
    53581 ttggcgcgtc cgggcggcac gctggcgaca tttacgtctg ccggttttgt ccgccgcggt
    53641 ttgcaggacg ccggattcac gatgcaaaaa cgtaagggct ttgggcgcaa acgggaaatg
    53701 ctttgcgggg tgatggaaca gacattaccg ctcccctgct ccgcgccgtg gtttaaccgc
    53761 acgggcagca gcaaacggga agcggcgatt atcggcggtg gtattgccag cgcgttgttg
    53821 tcgctggcgc tattacggcg cggctggcag gtaacgcttt attgcgcgga tgaggccccc
    53881 gcactgggtg cttccggcaa tcgccagggg gcgctgtatc cgttattaag caaacacgat
    53941 gaggcgctaa accgcttttt ctctaatgcg tttacttttg ctcgtcggtt ttacgaccaa
    54001 ttacccgtta aatttgatca tgactggtgc ggcgtcacgc agttaggctg ggatgagaaa
    54061 agccagcata aaatcgcaca gatgttgtca atggatttac ccgcagaact ggctgtagcc
    54121 gttgaggcaa atgcggttga acaaattacg ggcgttgcga caaattgcag cggcattact
    54181 tatccgcaag gtggttggct gtgcccagca gaactgaccc gtaatgtgct ggaactggcg
    54241 caacagcagg gtttgcagat ttattatcaa tatcagttac agaatttatc ccgtaaggat
    54301 gactgttggt tgttgaattt tgcaggagat cagcaagcaa cacacagcgt agtggtactg
    54361 gcgaacgggc atcaaatcag ccgattcagc caaacgtcga ctctcccggt gtattcggtt
    54421 gccgggcagg tcagccatat tccgacaacg ccggaattgg cagagctgaa gcaggtgctg
    54481 tgctatgacg gttatctcac gccacaaaat ccggcgaatc aacatcattg tattggtgcc
    54541 agttatcatc gcggcagcga agatacggcg tacagtgagg acgatcagca gcagaatcgc
    54601 cagcggttga ttgattgttt cccgcaggca cagtgggcaa aagaggttga tgtcagtgat
    54661 aaagaggcgc gctgcggtgt gcgttgtgcc acccgcgatc atctgccaat ggtaggcaat
    54721 gttcccgatg atgaggcaac actcgtggaa tatgcgtcgt tggcggagca gaaagatgag
    54781 gcggtaagcg cgccggtttt tgacgatctc tttatgtttg cggctttagg ttctcgcggt
    54841 ttgtgttctg ccccgctgtg tgccgagatt ctggcggcgc agatgagcga cgaaccgatt
    54901 ccgatggatg ccagtacgct ggcggcgtta aacccgaatc ggttatgggt gcggaaattg
    54961 ttgaagggta aagcggttaa ggcggggtaa tctgctctcg cagtgtttgc cggatgcggc
    55021 gtgaacgcct tatccgacct acgtgtgacg tgtaggcctg ataagacgcg ataagcgtcg
    55081 catcaggcat ggtgctccag acgccgcaac ttactgctgt gacgcctgct gaaacaggtt
    55141 ttcccacata tcggtcacca gcgcctggtc acgcggcgac aattcaccgg caccaatggc
    55201 tttttccaga ctctggctaa cggtcgtatg caccgcctga gcggagtggt catcaccact
    55261 ttccagttct gcgatggcta atgtcaggtg gccacgcaaa tacccactgg caaacaactc
    55321 atcatcactg gcatggtcaa ccataccgtc gattaatgcc agaatgcgtg attcaaactc
    55381 cgcgatcatc ttctttcctc attgtaaaac gtggcaccgg cttcagttga gcgcttccgg
    55441 ccacgggaac tgttccgccg taagttccgg cgtgtgataa taattttgta gtgctttaat
    55501 gaagcgtgcc ggacgctcgg gaatgccatt tgtcagataa tccatcacct gcgcatgtac
    55561 ccgccgttga aacaccacac gatccggttc gaagtcgcct tccagattgt cgcaactgac
    55621 gttaaacgga tatcccgccg ccacacagaa caaccagtcg agtgcctgcg gcttcacttc
    55681 aacatcttca aactggcttt gcgtttgggc atcacgccca tccgggcaat accagtagcc
    55741 gaaatcaacc agctcacggc gcgcttttcc ggcaatacac cagtgcgaaa tctcatgaat
    55801 tgcgctggca taaaagccgt gggcaaagac aattcggtta tacggtactt ccgcatcagc
    55861 aggaagatag atcggttcgt cgtcgccttt aatcagacgg gtattaaatt catcggcaaa
    55921 gcagctatta aaaatttcaa tcaactgctc atagtggtgt gtactgttca ttagttcatc
    55981 cccaaccagt ggaggatctc ctgtccgtgg ctatcgtaaa gtaatttggc actcatcacc
    56041 gccgagacga taacgatcat cgggcggatc agtttttgtc ctttgctcag caccagtctt
    56101 gaacccatgc gcgcgccgag gaactgcccc accagcatga caaagcccgt cgcccaaatc
    56161 actttgccgc cgagaataaa cagcagcaga ccgccaatgt ttgacgttgc gttgagtaat
    56221 ttggcgtgag ccgtggcttt ggcgaggttg aatccgcaca gcgtaacgaa ggccagcgcg
    56281 taaaacgatc cggcagccgg gccaaagaat ccgtcataaa aaccgacgca gccaccggca
    56341 attaacgcga agggtaaacc gtacatccgg cgctggcgat cttcttcacc cagctttggc
    56401 atcagcaaga aatagaggcc gatacaaatt accagaatgg gcaaaatctg ccgtaagaca
    56461 tcagcctgaa cgtattgcac cagcagtgcg ccgctcattg agccgacaaa ggtcatggcg
    56521 atattgagtt tctgatcgct taaactaacc actttgcggc gaataaagta gatagtagcg
    56581 gaaatagagc cgccgcaggc ttgcagttta ttggttgcca gcgcattagc gggagacatc
    56641 cccgctgcca tcaatgccgg aatggtgagt aacccaccgc caccggcaat cgagtcgata
    56701 aatcccgcca gcatggcgac aaaaaagaga actcccagca acagcgggga aaccataaac
    56761 aggctattaa acgtttccat tagatcacgt gctcatccag tagcgcctgg caggaaggcg
    56821 gcaacggagg cggtgtcttc ttctcaggct ttgttgttcc cggttttgga ggtgcaaacc
    56881 agctttgcag ttctgccccg caaccatcgc ccggtggcgg taaaggttga tcttcacact
    56941 ccagactatc ggcagggcaa cgtaatcgta catgcatatg cgcgcgatgc tggaaccagg
    57001 gtcgcacttt gcgcaaccag tcgcgatcgg tgcccgcatc aaggcaaagt tgttgtttaa
    57061 tcgccggatt aacaaaaatg cgcgtgacgt ctttgtcctg ggcggcgagt ttgatcaagc
    57121 tgaaaatttc tggcttccac agcgtggaga caacgtgttt accgtcgcgg gaaactaagt
    57181 ccagtgcttg cgggcgcaag agctgcgcgg aggtccagcg agttttcggc agttgcagaa
    57241 agatatcgac atccagtccg gtctggtggc tggcatgacc gccgttgaaa cgcccaccag
    57301 cgggcatccc catatcgcca atcagcaccg tacccatgcc cagattgctc acctggctac
    57361 tcagacgctg gataaacatc accagatccg ggtgaccgaa atagcgacgc tgatcggtac
    57421 gcatgacctg ataatgttcg gactgtatcg gcaacgtgtc agcgccgaca atacagccat
    57481 tagaaaaact gcctatcgat tgtgcgctac ccggcacagg ttgggttatt ttttgccacg
    57541 gcgtcgctgc caggctagcg ctactggcaa gcagagccag cagcgcaatc gcggttttgt
    57601 tcatttttta ccagcgtgga atatcagtct tcacatcggc attttgcgcc cgctgccgta
    57661 acaggtgatc cattaaaacg atcgccagca tcgcttctgc gatcggcact gcacggatcc
    57721 cgacacaggg atcgtgacgg cctttggtga tcatctcaac ttcttcgcca aagcggttaa
    57781 tagtgcgccc cggcacggta atgctggagg tcggtttcag cgccatatgg gcaatgattt
    57841 gctgcccgct gctgataccg ccgagaatgc cgcccgcatg gttgctctgg aaaccgtctt
    57901 tggtgatttc gtcgcggttc tggctgccgc gcagcgccac tacgtcaaaa ccgtcgccaa
    57961 tttccacgcc tttcaccgcg ttgatgctca tcagcgcatg ggcgatgtcg gcatccaggc
    58021 ggtcaaagac cggctcgcca agtccggcgg ggacaccact ggcaacaacg gtgactttcg
    58081 cgccgatgga gtcgccctct tttttcagcg cgcgcatcag ttcatctaac gcgtcgattt
    58141 tgtcggggtc cgggcagaag aacggatttt gttcaaccag cgaccagtct ttgatttcca
    58201 gcggaatgtc gcccatctgg gtcaggcagc cgcggatttc aataccaaat ttctcggcga
    58261 gatatttttt ggcaatcgcc cctgccgcca cgcgcatggc ggtttcgcgg gcggaagaac
    58321 gtccaccgcc gcgataatcg cgcagaccgt atttttgttc gtaggtgtaa tcagcatggc
    58381 ctggacggaa gacgtcttta attgcgctgt aatcctgaga acgctggtcg gtattttcga
    58441 tcaacaagcc aatgctggtg ccagtagtaa cgccttcaaa aacaccggag agtattttga
    58501 cctgatccgg ctcgcggcgc tgggtggtat agcgcgatgt cccagggcga cgacgatcaa
    58561 ggtcatgttg caggtccgct tccgtcagcg gaatgcctgg cggaacacca tcgacgatgc
    58621 agccgagcgc cagcccgtgc gattcgccga aggtggttac gcgaaagagt tgtccaattg
    58681 tgtttccagc catcacggct ccgttattgt tgtgtttgcg tgtttactta atctttataa
    58741 atcgcgaaat gttctcgtgc ggcaataagc tgctctttag tgagcataaa cacaccatcg
    58801 ccgccgttat caaactccag ccaggtgaac ggaacatccg gatattgttc cataagatgt
    58861 accatgctgt tgccgacttc acaaatcagc acaccatcat cagcaaggta atctgccgcg
    58921 ttaccgagaa tgcgacgcgt cagtttcagg ccgtcggtgc cagatgccag gcccagttcc
    58981 ggctcgtgac ggtattcgtt tggcaggtcg gacatatctt ccgcgtcgac atacggcggg
    59041 ttagtgacaa tcagatcgta ttgcactttc ggcaaatcac ggaacagatc ggaacgaatc
    59101 ggaatgacgt tgtggatcag accgtgttct tcgatgttct gttcggcaac cgccagcgcg
    59161 tcgggagaga tatctaccgc gtcgacttcc gcttccggga aggcataagc acaggcaatg
    59221 gcgatgcagc cgctaccggt acacatatct aaaatatgct gcggttgctt gctgatgagt
    59281 ccggcaaatt tattgttgat cagttcacca atcggcgagc gcggcaccag cacgcgttca
    59341 tcgacgtaaa attcatgacc gcagaaccag gctttgttgg tcaggtaagc caccggaatg
    59401 cgctcgttga cgcggcggat cacgcgttca acaatacggt gtttttcgct ggaggtcaga
    59461 cgcgcggtgc gcatatcttc cgggatatcc agcggcaggt agagcgatgg taacaccagc
    59521 tgtacggctt catcccacgg gttatcggtg ccgtgaccgt accagatatt tgccgcgctg
    59581 aagcggctta ccgaccagcg caacatgtcc tgaatggttt gcagctcatt tactgcttca
    59641 tcaacgaaaa ttttatccac gtattcctcc agggcatgat cgcaataatt tcggcggcta
    59701 gtttgccatg aagatgacga taaatcagca ttcacgcgcg gtgagtgagg aaaaatacgt
    59761 ttaaaacgat cgattgtgct acgagtcggg taaactgtag gaaaattaga aatagagaca
    59821 gataaatgaa aaagaaaaca acacttagcg aggaggacca ggctctgttt cgccagttga
    59881 tggcggggac tcgcaagatt aagcaggaca cgattgtcca ccgaccgcag cgtaaaaaaa
    59941 tcagcgaagt gccggtgaaa cgcttgatcc aggagcaggc tgatgccagc cattatttct
    60001 ccgatgagtt tcagccgtta ttaaataccg aaggtccggt gaaatatgtt cgcccggatg
    60061 tcagccattt tgaggcgaag aaactgcgcc gcggcgatta ttcaccggaa ttgtttttgg
    60121 atttacacgg tctgacgcag ctgcaggcca agcaggaact gggcgcgttg attgccgcct
    60181 gtcgccgcga acatgtgttt tgtgcctgcg tgatgcatgg gcacgggaag catattttga
    60241 agcaacaaac accgctgtgg ctggcacaac atccgcatgt gatggcattt caccaggcac
    60301 cgaaagagta cggcggtgat gcggctttgt tggtgttgat tgaagtggaa gagtggttgc
    60361 cgccggagtt gccctgaatg ctggcagcaa atgccggagc agcgtaaacg ccttatccgg
    60421 cctacaaata tgtgataatt caataaattg caattcactt cgtaggcctg ataagacgca
    60481 tcagagcaat actacgaact acagttcatt caatacatac tgaaaacgac acgtcgaatc
    60541 aatcggttgc ccactactgg tattcacatt caccacaata tcgtgtagta ctttatccgt
    60601 taccggatga cgagttggcg taaccgttac cgttgattta tcacaaccgt aagaggtaaa
    60661 ggttaacaat tctgataccg cgcggcagta tgctcctctg ccatcattat cagaaaaatt
    60721 actgccatca cagagtctgc cgccttccat caagtaggtc gaaagcgtgg tggtcactgt
    60781 tgaccccgtg gactgaattc gtaacgcctt attatggctg acataatttt cactccagga
    60841 gatatcgtat gaagcgaccg gtatcgttgg gcatattgtg ttatgcactg taggggccgt
    60901 atagcttcca aatccgggta aatctgtata ggaataaaag gatgcgatcc aagggcaacc
    60961 ataatcatca gacgcaacgg taattttaaa cgtcatatat gcacaacgaa aaattttcgt
    61021 atctatgcca gggcaatttg agttattaat tgtatcaaag tcagtcagta ccaaagttgg
    61081 agctgaaccc gacccagacc aggaaataac ccgcaacgta tcgcctttat acgcaaaaga
    61141 atcatatttc ccatatgcaa cctcctttgg cgttatcacc gtaacattga aaacactgct
    61201 cgctacagta ccggttacct tgtccatggc ataataaact ctcacactgt caaaactcgc
    61261 actcataagt ttatctccag cataactctt agatgcgccg acaacactca gaacgagcag
    61321 cattaggctc gcgaaaatta tggtccattt tttcatttca gaaatactca aaggttgccg
    61381 tcactgtggc actaaagtct ccggacgtta catctcgccc gctctttgcc tgtaaccagg
    61441 tattaaaatt taggctgtta tttccctgat tcagcgggag cgtgacgcct gttgtattgt
    61501 tgattggcac cgatgtgcct gccgcagttt caataccaat cccagcgccg gatgctgatg
    61561 acgaggagtc aaaggccaga aagccgggta gagcactatc ttctgttcct gtcaacgtta
    61621 ctttgacatt aaacaatgta gaactatgac aatctttcaa ttgaaacacc aatggcactc
    61681 gctctgactg cccaaggttc attaaatcgc tggcggcaat ggcaggaaaa tgtacttctg
    61741 ccaactggct accattaatc actggcgtac acgatttact gattaaattg ccggtaaagt
    61801 gcaaattatc tcctgctatt gtggttccac tgcccagcaa catcaggcta ctaataaaaa
    61861 acaacgtacg ctttttcttc attggtaatc tacctgtaac gtggcccagg cctcaaagtc
    61921 cgcctctggc agaacgacac cactcttttt tactggcaca gccgttaaca ccggtaaatc
    61981 ggtttcattc accataagtg gcgtattgat cgtaaaactc tgccctgcct gtttgagctg
    62041 aatgcctaaa ccagtgatat tgctgcttac tgcagcatta tcaaaatcgc ttggtgtgcc
    62101 actccagctc aaggtcattt ccattaatgg gtcgcgaacc gccgtatcgc aggtaatgga
    62161 atacggcacg gtttgctcat aattgctgcc attaatgtca tcaataagca catcctgaaa
    62221 agttatttca atggtttgag cgttgctaat cgtgcagtta ggcggctgca ctaaataacc
    62281 gtgaaaagag acgtttgaga gtgccatcga gcaaaagccc cacagcaaaa taagcgagat
    62341 ccgtttcatt ggtaatccac cacaattgtg gcactggcgt taaattcagc tgccgctaac
    62401 gttgtaccac tctgtttcac cggcacagct tccagaacgg gaactcccga ggaaaaatta
    62461 aatggcaacc atgagccaga ggttaagtcg agtattgtgt gatcactact tttctgaacc
    62521 cgaataccaa acccaggaat acccgtgctg ataactgttt caccgttaat cacgaccgtt
    62581 gtcgcctgca tctgtagacg caggtcatcc attgccagat tgttacaaac aagcgagtag
    62641 tcaatgggac gtttataatc aaccccattg atcttattaa tgaatacatt accaaactca
    62701 actgccgcac catttaccgt acaaggagga ggggcatcga caagcacccg taaattcata
    62761 cttactgact tactcgctgc aactgcaaag ccgctaccaa cacaacatat cagtaaccca
    62821 gttaccgccc agacagaaca agatcctttc atactggctc tcccggcctc agcctttgcg
    62881 gctctgctct tcatcgacct ggcaactgtt accgttgcaa gtgaaaaaga gcggcatccg
    62941 agcgccatag tcgttgacat aggtcagtac cggcacgcta cccatcttca catttagcgg
    63001 tgcagaggtt ttcggcgaca tcactattgg cgagaaaccg gctgcaggat tgccattttt
    63061 ctgtgtgctg gcattactga aaatgacgta atacggcgtc gggttattga cggtaaattc
    63121 ctgaccgttg cgggttaaag taaccttata ttgccagggg tttttcatgc tgacgttctc
    63181 cagcgccttc ggtcgccaga acaacttaat acgcgtttgt agtgctattt gcagcgtgtt
    63241 ggctttgttt gattttggcg ggatctcacg cacgttgaag tagaacaggc tttcacgatc
    63301 ggcaggcaat ttgttgatat ccggcattcc ctgcactttt acctgcccgt tcatcattga
    63361 atcgatgcgt tgcaccggtg gcagaacagt taacggtgaa ctgatcttgt tacccttttc
    63421 atcttcgatc cagctttggg ccagataagg cagtttcgga tcgttattgc gcaacgtcac
    63481 gctgattgat ttgtcgcttt cattaaatat caaacgggta cgatctggcg ttacagaagc
    63541 ctgggcagac aaactggcag cgctaagcag taacgctagc gtcgtagcac ccaatttggc
    63601 agagcaaagt aaatcagaca tggatcattc cttaatcacg ttattggtta gctgaaacag
    63661 aaacaggggg attggtgggt gtcacctgtt gcgtctgctc ctgaatgaca ggctttatgt
    63721 catcaggaac aaccggcgcg acgttgcctt tatgctggca tggcagtaac aagccgttaa
    63781 acagatcggc aggcagcggg tccggcaggt tgatatcgca atgcgcaaca ccactccaga
    63841 acacactcat gtgttcgcca ggtttcaccc cagccagata aacattgccg tcatcatcga
    63901 caaggcccac tgtctgctcg ttatcatttt ttacttctgc gccaaacgga ggatggctgc
    63961 cgtcttgtaa acgcagcacc gccatcgctt tttgaccact gatgacggca aatttgcggt
    64021 agccaatcgc cccttcagtt agcgtggctt gtaccaccga ctgggttgct tcggcgtttt
    64081 ccggcagttt gttgaggtcg atatacgcct gattgcggta atagttattg acgtcagaaa
    64141 cgacggcttt accaaacata ttggtataaa cagcagcacc gttaccttct accggaacat
    64201 cggcaacgcc atcggcatca atcaacaagc gtgtcccgcc catattctgg gtacggtgaa
    64261 gtgcgccacc gtgggcagta agcgttgcgc cgccctgtaa cgacaagccc gcagaggtgt
    64321 attgcccttc atggtagttc gcactgaggt caacctgcgc cagcgaacca tcatggctgt
    64381 aatagcagtc aacgctggtg tgtttgtcac tggtgccgac gttcaactga tagtgagtcg
    64441 cgtcatcgac acggctgaaa taaccgacct gactgctgtc cgtcccactg ccatagttac
    64501 cgttatagct aacggtactg ttgtcgcccc acggcatact gagcgaaatg tacatgcctt
    64561 tgtcggcctg gttgtcatac tcgtaacggt agccagtcat cgagatgctg acattacgaa
    64621 tactgcccat attgaaatag tgcgagagca tgatgttgta gtttgtctgc tcctcgcgat
    64681 cccagtaggt atggcgggta tagttgagat aaaccgaaac acccgcatcg cggaagttct
    64741 ggttataagt ggcggtgtac atctctttgt cgttgcccgt gcgtgccatt ccgctgtcac
    64801 tggcatccag atactcgctc atggtcataa agttctcttc cgagaagcga tatccagcaa
    64861 aagtaacgcg gctgttgagc tggtcaaagt ctttggaata actcacacgg aaggagttac
    64921 cgtccagcga acctttgcca taagcggtgt ctttatccag tttggtgtgc gagtgagtaa
    64981 catcaaacgc gaccgcgccg aatgtagaca aatcgcgacc gacgccaagc gccgcagact
    65041 gatagttttc atctcccagt gcgccgccat ataacgacca gccgttagcg atcccccagg
    65101 aagcttcagc accagaaaaa aattcaccct cgacatggtg tccccactct tgcggacggc
    65161 ccatcatgat cttatagcga acctggcctg ggcgagtgag gtatggcatc gaggcggtgc
    65221 tgatgtcata ttcctgcacc tggccgttct gttcttcaat gcgaatatgc aacgtaccgg
    65281 agacggaatc accaagatcc tgaatacgaa acggtccagc cggaacctgc gtttcgtaaa
    65341 tcacacgccc catctggctg acggtcactt ttgctgtggt gtgtgccacg ccggaaatgt
    65401 ctggcgcgta gccgcgcaga ttgggaggca acatttgatc gtcagtactg acactgccac
    65461 caacatagtt aaaaccatca aaaatatcgg atctgaggta atcctcgccc agcgccagtt
    65521 tggcttttaa tgatggtaac gcccgccagg catagtagcg actccactcc catttttttt
    65581 gagtttcatc gccgctgaat tcatcgtcat cattactgcg agtatgttga tagttagtct
    65641 gccagtcagc acgcatacgc cacggcccca ggttaacccc gaccgtcccg ttgccgctga
    65701 tctcgttact atcgtcaccg ccattttctt cgtgccgggt ttgtgcgttg atgctgtagt
    65761 ccgcgacgat cccggagatg ccgtcatccc aacgtgaagg cggatcccag tcgggataag
    65821 tatattcgag gtaagcctgt ggcagtgaaa tgactaatgc ggactggctt aaatcagcct
    65881 taatttccat gccttccagt tgaccggatt tcaggcattt agcatcgtgg ctccattgca
    65941 gatttttcgc cacgtcttct tttaaaccaa actgcgctac cagttccggt gtcagacaag
    66001 catagctttt gctcgcgtca tcttcaccag cataccagta aatatcgtac tcttccgcca
    66061 atggctgttt atttagttga acctgtaaat tgtattttcc gggctcaaca tatccctggc
    66121 tggaaaaacg cttcagatca atttttgtgt cgccttttaa ttccagaaaa cgggaatcaa
    66181 actgaatgtc gtcttcagcc cagacactgc tataactccc tgacattgcc agcgcaatgc
    66241 accacggaag aatccgtaat cgaaaaagag aatggtcagg catacacgtt tctatccgtt
    66301 tttattcttg ttcggtcaaa atccatttaa atcccccata gcagtgtcgc tatgggggta
    66361 tgccttacag gtaagtaatc tggaaggtgg tatttgcttc gaaattaccc agatctggcg
    66421 catcagctgc gccgaccagc caggctttaa agttcagtgt ctgcttcgct ttgcctttat
    66481 tggtagaagt gtctttcgct attttctggt caatacccat gccgcttttg taagaggtag
    66541 ccagagagtc accgctcgcc aggctaacat tgttaaatgc cgcaccggta tcggtattga
    66601 aaatggtgta ataattgccg ctatttgcag aagaaacggt accagtaaag gtagtggtca
    66661 ttgtttcctg agtatcaaat acgcaatctt gcagacgaat ctggaaactt ttcggcgtgg
    66721 tggtgccgtt atttttcagt ttttcggcac cgatatcacc catatcaact tccagtttgt
    66781 gatcgtccgg aacgatagaa cacactgagt cttcaatggt gccgtaaaaa cgtgcagtac
    66841 cgttgttacc cgcagcgatt gttgccgtag aggtgaacac gcccatcacc attgccgcag
    66901 caatagctgt tttaacaaac ttactcatta tttgtcctta aactataaat aatgaaaaat
    66961 ataaacattg caatacattg attcagtcaa tagccaatgt ttatacccag ccattgaata
    67021 cggcatgcga tccaaaaaga acaggtcgta gaaaattgta ttcgttggtg tggggccgca
    67081 tagtgatgtt tccacacccc tgatgcaatt taattttact aaagagtaaa tgtagattta
    67141 attaatatat tgacagaaga aaggttatct taaggacatc cttaaaaaac acggaataag
    67201 tatttagaaa aaatagaaag tatatcaata atgaatatca acagcttaaa aaacaaacaa
    67261 tgaacatata atgcgattgg catttatcca cttttgacaa tataatatat aaacactata
    67321 gccagaattt aatcaagttt caattacatc ctgcctgatt attcaactct attatctgta
    67381 ttaataaatg acaatgagtg tcaaacagac attattcatt tcaacatgta actttgggca
    67441 aaatcaagat acgcgcagaa gaataatttc cgaatgaatg tagaaagtgt tttatttata
    67501 gataaggaga gtcttcgata gtagtgaggt tgacagatat ccgctgcctg atgagcaatt
    67561 atgcccaatg aatctacctc attgggcata atttggaaca catcagatag cttttgccat
    67621 cttcagatta cacgggctca tctgccagtt gaatgtaccg tttccgcttt catcaagcgt
    67681 tacgctggca atggccgagg tggtaaacat aggcggcgtt tcgcccggac ataactcggc
    67741 aaccagatac ccgactaacg gtaagtgaga gatcaccagt accgaagcaa caccttcatt
    67801 ggtcaacgcc tgcaaatagg cactgaccag accaacatcg ccgcagggcg ttaactccgg
    67861 cagaacttct gcactggaag gcaggttcag acaatcccct acttcttcca gtgtttgctc
    67921 ggctcgcagg aacgggctca ccagaacgcg ttcgatttcc actttttgac ctttcagcca
    67981 gttcgccatc aggcgagatt cgtcacaacc attagtggtc agaggacgaa ccgaatcact
    68041 ggcggcatcg agggctgcgt cgccgtgacg catgataaaa acttgcatat tgcaccgctt
    68101 ttgttaacca gattcgcccg ttttctttca tctgaaacca gagaaagaaa acggtggccg
    68161 gcattgtgcc ttatccattc accgaatgaa acgctgtttt ttacctcaat ggcgtaagta
    68221 tagtcaatat gcgtttacat tttgaccaac actccgctat tcagcgcgga ttcatatagc
    68281 tttgaccttc ttattgcagg tcagttgcag ttgttttcca aaaactttcc ccacgcgcgc
    68341 ccatctcgac caaacgctcg caaggggtaa aacgggaacc atactgcgtg gcaagtcgtt
    68401 gcattattgc aaccacttcg cccgcgccga gagaatcgat atagcggaac ggtccaccga
    68461 gaaatggcgg aaaaccaatg ccaaataccg cgccaatatc cccgtcacgc acgctacgga
    68521 taacctgctc atcaacacaa cgtactgctt cattcagcat caacatcaca caccgttcag
    68581 caacctgcgg tgcggagagt cgcccctgcc cttgtgcgcc aatgagcggg taaatggcag
    68641 gatcgacctg ttttttgctt ttacgccctt tctgaccata aagatagaaa ccccgaccat
    68701 tttttctgcc tttgcgatcg tcgttcaaaa ttgaagaaac aacatttgca ggcgcgctaa
    68761 aacgttctcc ataagcggct tccagtacag gaataatttt agtcccggtg tcgattccta
    68821 cctcatccaa aagttggatt gggcctaccg gaaaaccaaa tttcactagc gcagcatcaa
    68881 tgtgctctac ccgttcacct tcggtcagca tgcggatagc ttcattaatg taaggcgcta
    68941 agatgcgatt gacgtaaaac ccggctttgt cacgcacgac aatgggcgtt ttgccctgtt
    69001 ttttcgccag ttttaccgta gtggcgatgg tttgcgctga tgtacccgca tgaggaataa
    69061 tctccaccag cggcattttt tccaccggac tgaagaaatg caggccgata acttgctcag
    69121 gtcgcgtggc gtgagcggcg atatcaccaa tcggcaaaga tgacgtattc gaagcaaaga
    69181 tggtatgagc ggcgcaattt tgctccactt ccgccaccat ctgttgtttt aattcgagat
    69241 tttcaaacac cgcttcaata atcagatcgc gatgggcaaa gccgcgatag tccgtcgttc
    69301 cggagattaa tgccagctgt ttgtcacgtt cgctggcttt gagatgacga cggcgaactt
    69361 tgccctccag ctgatcccaa ctgtacttca gcgcatgatt tatgccctgc gggttgatat
    69421 ctttaattct gaccggaagc cccgctttac aagcagtgac ataagcaata ccgccgccca
    69481 tcaagccacc acctaaaatt cccacgctgt ttaatggcgc aggcggtgca tcactaccgg
    69541 gatctttctt cacttccgta ctggcaaaaa agatactacg caaagcttgc gattgtggcg
    69601 tcatcgccag ttcgccaaac gcccgagctt cggcgtcata accgctgctg gtgccctgcg
    69661 ctaatcccgt ttcaacaacc tccaggatgc gttctgtcgc cggataattg ccttgagttt
    69721 tgtgttctgt tttcttgccg accattttga acagcagcgc acgacctaac ggccccgcca
    69781 gaatacgctc gcgtacaggt agagggcggg aagatgggcg atccttcttt gccagctcaa
    69841 cagcggcttc cagcagaatg gagtgcggaa caacgtcatc caccagcccc agctttaatg
    69901 cctgtttcgc ccgaagttgt tttccggtga ggatcatctc taatgctgtg ctgacgccta
    69961 tcagacgcgg taaacgctgg gtgccgcctg aaccgggtaa caatccaagt tgtacttcag
    70021 gcaaaccgag caccgtttta ggatcgtcag tacaaacgcg accgtggcac gccagcgcca
    70081 actccagccc accacccagg caagcgccat gaatagccgc gataaccgga atgggcaaag
    70141 catgaatctc cgccatcaac tgttggccct gccgcgccag tgcttccgct tcttgcgccg
    70201 ttttgcagtt gccgatcatg ttgatgtctg cgccagcaat gaagttgtcc ggtttagcgg
    70261 agacaaacac cacgcctcgc aactctttgt tttcacggag ttgcttaata atggcgcgca
    70321 cctgcgaggc aaactccgcc ttcagggtat tcattttctc acccggtacg tcgatggtga
    70381 taacggcaat gttgtccaga cgaacattaa gggtaaacgc tgatgtcatt tccattattc
    70441 cgcctccaga accattgccg cgccaagccc accggcagca caggcggtaa ctaaaccaaa
    70501 tccaccgccg cgacggcgaa gttcatgcaa tgtctgggta atcatccgtg cgccggtcgc
    70561 cgcgaaggga tgcccgtaag caatcgaacc gccaagcacg ttaaatttgc tatcgtccac
    70621 ttcgccagtg gcatgtgcac gccccagtac atcacgagca aaacgttcac tacccagcaa
    70681 ctgaatattc gccagcgtct gagctgcaaa ggcttcgtgc atatcgaaca ctgtcagatc
    70741 gctcatcgtc aaaccggcac gttccagcgc cagcggcgtt gaccaggccg gaccgagcaa
    70801 catgtcctgc cagacatcaa tcgcagtaaa tgcgtagctg cgcagatacc ccagcggtac
    70861 cagccctaat tctttcgccc gggattcagt catcaggatc accgccgctg cgccatcggt
    70921 cagcggcgta ctgttcgccg ccgttaccgt tccgtgtttg cgatcaaacg ccgggcgcag
    70981 ctttgcgtaa tcggcaagcg aggaattacc gcgaatattg ttgtcttcga caagcggttg
    71041 tttataagga gggataaagg cagtcatcac ctcttctttg agtttaccgt ctgaccacgc
    71101 ctgagcggca cgctgatgcg aacggtgcgc taatgcatct tgctgttctc gggtgatgcc
    71161 gtaggttttc gccatttgct ctgcggtgtc acccatccgc aagccggtag aatattctgc
    71221 taccgcaggc ggtacgggca ttaagtcgcg caaacgcagg cgagagaaga gtttcagtcg
    71281 ctggctcatg gttcgagctt tgttgacatc aaccagcacg cgcgccagtt ttttactgac
    71341 gccaattggc aataccgaag aggaatctgc cccaccggca atccccgctc ggatagttcc
    71401 cgccatcagg ctttctgcga cgtttgcaac cgcctggaaa ctggtagcgc aagcgcggct
    71461 gacgctgtaa gcatcggtat gcacattcat tccggtgccg agaacaattt cacgcgcaat
    71521 gttgggggct tcaggcattt gtacgacctg accaaagacc agttgttcaa tcacttcggc
    71581 ggggatctcg ctgcgtgcca gcaattcgcc taccaccatc ttccctaaat caaccgcggg
    71641 aatgccatga aaagccgtcg cctgacgggc aaaaggcgta cgtaaaccgc taacaatggc
    71701 gatacgatcg ccctggcggg taaccagcgg taaaacctga cccataacac tcccctgtaa
    71761 aaaaataaat aaagtggtct gacctgatca tagtcttaac cattttttta catttagcca
    71821 agtggagaaa agggaaagtg ggagctatga cacagagaga aaagaagaag agaaaagaaa
    71881 acgcccctgc cagcaactga caggggcgta cgtacccgag aggaattaac gcagacccag
    71941 ctggaagatc agcatttcag cttcgcaggc aaaagtgaaa tcgatatcca ggcgcacacc
    72001 gtcagactct tcagtgaaag tcggggtgat tttgcacggt tcggattcca cgctacgggc
    72061 tttttcagtc agcgccgcca gcgtttgttc tgcttctgcg cggcttgcaa acacgcggct
    72121 gtaagacgcg gtgcagtcgg agttgtccat aatggtgcca acatccatac agcagcaaac
    72181 cggggtttca tcagcactac atttactcat cgttgatttc ctctgtatgt gcacccaagg
    72241 tgccagataa acgttgtgga tattttacgc ttccggaaag tgctgctcca gttgttaatt
    72301 ctgcaaaatc ggataagtga ccgaaatcac acttaaaaat gatctaaaac aaaattcacc
    72361 cgaatccgcg agtgcgcaac ctccaaattt tgccagctgg atcgcgtttc ttagatcata
    72421 tttgaaaaaa gatggaaaca tacttgcaac attccagctg gtccgaccta tactctcgcc
    72481 actggtctga tttctaagat gtacctcaga ccctacactt cgcgctcctg ttacagcacg
    72541 taacatagtt tgtataaaaa taaatcattg aggttatggt catgagccag aaaaccctgt
    72601 ttacaaagtc tgctctcgca gtcgcagtgg cacttatctc cacccaggcc tggtcggcag
    72661 gctttcagtt aaacgaattt tcttcctctg gcctgggccg ggcttattca ggggaaggcg
    72721 caattgccga tgatgcaggt aacgttagcc gtaaccccgc attgattacc atgtttgacc
    72781 gcccgacatt ttctgcgggt gcggtttata ttgacccgga tgtgaatatc agcggaacgt
    72841 ctccgtctgg tcgtagcctg aaagccgata acatcgcgcc tacggcatgg gttccgaaca
    72901 tgcactttgt tgcaccgatt aacgaccaat ttggttgggg cgcttctatt acctctaact
    72961 atggtctggc aacagagttt aatgatactt atgcaggcgg ctctgtcggg ggtacaactg
    73021 accttgaaac catgaacctg aacttaagcg gtgcgtatcg cttaaataat gcatggagct
    73081 ttggtcttgg tttcaacgcc gtctacgctc gcgcgaaaat tgaacgtttc gcaggcgatc
    73141 tggggcagtt ggttgctggt cagattatgc aatctcctgc cgggcaaact cctcaagggc
    73201 aagcattggc agctaccgcc aacggtatcg acagtaatac caaaatcgct catctgaacg
    73261 gtaaccagtg gggctttggc tggaacgccg gaatcctgta tgaactggat aaaaataacc
    73321 gctatgcact gacctaccgt tctgaagtga aaattgactt caaaggtaac tacagcagcg
    73381 atcttaatcg tgcgtttaat aactacggtt tgccaattcc taccgcgaca ggtggcgcaa
    73441 cgcaatcggg ttatctgacg ctgaacctgc ctgaaatgtg ggaagtgtca ggttataacc
    73501 gtgttgatcc acagtgggcg attcactata gcctggctta caccagctgg agtcagttcc
    73561 agcagctgaa agcgacctca accagtggcg acacgctgtt ccagaaacat gaaggcttta
    73621 aagatgctta ccgcatcgcg ttgggtacca cttattacta cgatgataac tggaccttcc
    73681 gtaccggtat cgcctttgat gacagcccag ttccggctca gaatcgttct atctccattc
    73741 cggaccagga ccgtttctgg ctgagtgcag gtacgactta cgcatttaat aaagatgctt
    73801 cagtcgacgt tggtgtttct tatatgcacg gtcagagcgt gaaaattaac gaaggcccat
    73861 accagttcga gtctaaaggt aaagcctggc tgttcggtac taactttaac tacgcgttct
    73921 gataacgcgt tcgcctggat aaagtcacct gcatagcagg tgactttaac tcccccactt
    73981 caccgaaagt agtgtcctca ttgcttacct cctgagtttt gcaaacagcc tgttggcagc
    74041 ttgccctttc aaatcagtaa gtggtatcca cagacaaacc tgaagggaaa tgcatatttt
    74101 caagcgttct gctcgtcctt ctcaaagagt ttacttttct gcatttccag gatactcaac
    74161 ccccctggct attgtgcgct tatacactca aattaaagat aggttctaaa taaatgagcg
    74221 ttttttgata gtctatttca ttaggtaata tatatttgta acaaatcaat caacatggaa
    74281 taaaatcatg ctaccatcta tttcaatcaa caataccagc gcagcttacc cagaatccat
    74341 caatgaaaat aacaatgatg aaattaatgg attagtacaa gagttcaaaa acctttttaa
    74401 tggtaaggaa ggaataagca cctgtattaa acatctactt gagcttataa aaaacgccat
    74461 acgagtaaac gacgatcctt atagatttaa tattaataat tcctcagtta cttatattga
    74521 tattggctcc aatgatacag accatattac tattggtatc gacaaccaag aaccaataga
    74581 attacctgcg aactataaag acaaagaact cgtccgtact atcattaatg acaacatagt
    74641 tgagaagact catgatatca ataacaagga aatgatcttc agcgcattaa aagaaatata
    74701 tgatggagat cctggtgtta tcttcgataa gatatcacac aaactcagac atacggtaac
    74761 ggaatttgat gagagcggga aaagcgaacc aacggactta tttacctggt acggtaaaga
    74821 taaaaaaggc gactctctcg ctattgtaat taaaaataaa aacggaaatg attacttatc
    74881 tctcggttac tacgatcagg acgactacca cattcaaaga ggaattcgta ttaatggtga
    74941 tagtctcacc caatattgta gtgaaaacgc caagagtgct tcagcttggt ttgaaagcag
    75001 taaagctatc atggcagaat catttgcaac tggttccgat catcaggttg taaacgagct
    75061 caacggggaa agactgagag aacctaacga agtttttaaa cgtttaggtc gagcaataag
    75121 atataatttt caagtggacg atgcaaaatt tagacgcgat aatgtaaaag aaataatttc
    75181 tactttattc gctaacaaag ttgacgttga ccatcctgaa aataaataca aagactttaa
    75241 ggatctcgaa gataaagttg aaaaaaggct tcaaaatcgc cagacggaat atcaaaatga
    75301 aa
//
LOCUS       DAZKHC020000009        72892 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZKHC020000009 DAZKHC020000000
VERSION     DAZKHC020000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 72892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.009"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..95)
                     /locus_tag="ACHOSM_RS03730"
                     /old_locus_tag="pgaptmp_000746"
     CDS             complement(<1..95)
                     /locus_tag="ACHOSM_RS03730"
                     /old_locus_tag="pgaptmp_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557326.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNG"
     gene            complement(180..500)
                     /gene="chbB"
                     /locus_tag="ACHOSM_RS03735"
                     /old_locus_tag="pgaptmp_000747"
     CDS             complement(180..500)
                     /gene="chbB"
                     /locus_tag="ACHOSM_RS03735"
                     /old_locus_tag="pgaptmp_000747"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HGU4557327.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVATIKK
                     AAAN"
     gene            complement(799..1137)
                     /gene="osmE"
                     /locus_tag="ACHOSM_RS03740"
                     /old_locus_tag="pgaptmp_000748"
     CDS             complement(799..1137)
                     /gene="osmE"
                     /locus_tag="ACHOSM_RS03740"
                     /old_locus_tag="pgaptmp_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HGU4557328.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1339..2166
                     /gene="nadE"
                     /locus_tag="ACHOSM_RS03745"
                     /old_locus_tag="pgaptmp_000749"
     CDS             1339..2166
                     /gene="nadE"
                     /locus_tag="ACHOSM_RS03745"
                     /old_locus_tag="pgaptmp_000749"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGU4557329.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2396..3283
                     /gene="cho"
                     /locus_tag="ACHOSM_RS03750"
                     /old_locus_tag="pgaptmp_000750"
     CDS             2396..3283
                     /gene="cho"
                     /locus_tag="ACHOSM_RS03750"
                     /old_locus_tag="pgaptmp_000750"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HGU4557330.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3243..3818)
                     /gene="ves"
                     /locus_tag="ACHOSM_RS03755"
                     /old_locus_tag="pgaptmp_000751"
     CDS             complement(3243..3818)
                     /gene="ves"
                     /locus_tag="ACHOSM_RS03755"
                     /old_locus_tag="pgaptmp_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HGU4557331.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQI
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(4021..4506)
                     /gene="spy"
                     /locus_tag="ACHOSM_RS03760"
                     /old_locus_tag="pgaptmp_000752"
     CDS             complement(4021..4506)
                     /gene="spy"
                     /locus_tag="ACHOSM_RS03760"
                     /old_locus_tag="pgaptmp_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HGU4557332.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4836..5804)
                     /gene="astE"
                     /locus_tag="ACHOSM_RS03765"
                     /old_locus_tag="pgaptmp_000753"
     CDS             complement(4836..5804)
                     /gene="astE"
                     /locus_tag="ACHOSM_RS03765"
                     /old_locus_tag="pgaptmp_000753"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HGU4557333.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5797..7140)
                     /gene="astB"
                     /locus_tag="ACHOSM_RS03770"
                     /old_locus_tag="pgaptmp_000754"
     CDS             complement(5797..7140)
                     /gene="astB"
                     /locus_tag="ACHOSM_RS03770"
                     /old_locus_tag="pgaptmp_000754"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HGU4557334.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(7137..8615)
                     /gene="astD"
                     /locus_tag="ACHOSM_RS03775"
                     /old_locus_tag="pgaptmp_000755"
     CDS             complement(7137..8615)
                     /gene="astD"
                     /locus_tag="ACHOSM_RS03775"
                     /old_locus_tag="pgaptmp_000755"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HGU4557335.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8612..9646)
                     /gene="astA"
                     /locus_tag="ACHOSM_RS03780"
                     /old_locus_tag="pgaptmp_000756"
     CDS             complement(8612..9646)
                     /gene="astA"
                     /locus_tag="ACHOSM_RS03780"
                     /old_locus_tag="pgaptmp_000756"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HGU4557336.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEARDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9643..10863)
                     /gene="astC"
                     /locus_tag="ACHOSM_RS03785"
                     /old_locus_tag="pgaptmp_000757"
     CDS             complement(9643..10863)
                     /gene="astC"
                     /locus_tag="ACHOSM_RS03785"
                     /old_locus_tag="pgaptmp_000757"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HGU4557337.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11309..12115
                     /gene="xthA"
                     /locus_tag="ACHOSM_RS03790"
                     /old_locus_tag="pgaptmp_000758"
     CDS             11309..12115
                     /gene="xthA"
                     /locus_tag="ACHOSM_RS03790"
                     /old_locus_tag="pgaptmp_000758"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGU4557338.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCIETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12282..12992
                     /gene="ydjX"
                     /locus_tag="ACHOSM_RS03795"
                     /old_locus_tag="pgaptmp_000759"
     CDS             12282..12992
                     /gene="ydjX"
                     /locus_tag="ACHOSM_RS03795"
                     /old_locus_tag="pgaptmp_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGU4557339.1"
                     /translation="MNAERNFLFACLIFALAIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            12997..13674
                     /gene="ydjY"
                     /locus_tag="ACHOSM_RS03800"
                     /old_locus_tag="pgaptmp_000760"
     CDS             12997..13674
                     /gene="ydjY"
                     /locus_tag="ACHOSM_RS03800"
                     /old_locus_tag="pgaptmp_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HGU4557340.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13689..14396
                     /gene="ydjZ"
                     /locus_tag="ACHOSM_RS03805"
                     /old_locus_tag="pgaptmp_000761"
     CDS             13689..14396
                     /gene="ydjZ"
                     /locus_tag="ACHOSM_RS03805"
                     /old_locus_tag="pgaptmp_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGU4557341.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14396..14944
                     /gene="ynjA"
                     /locus_tag="ACHOSM_RS03810"
                     /old_locus_tag="pgaptmp_000762"
     CDS             14396..14944
                     /gene="ynjA"
                     /locus_tag="ACHOSM_RS03810"
                     /old_locus_tag="pgaptmp_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGU4557342.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTDSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14954..16120
                     /gene="ynjB"
                     /locus_tag="ACHOSM_RS03815"
                     /old_locus_tag="pgaptmp_000763"
     CDS             14954..16120
                     /gene="ynjB"
                     /locus_tag="ACHOSM_RS03815"
                     /old_locus_tag="pgaptmp_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4557343.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKVNPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            16093..17628
                     /gene="ynjC"
                     /locus_tag="ACHOSM_RS03820"
                     /old_locus_tag="pgaptmp_000764"
     CDS             16093..17628
                     /gene="ynjC"
                     /locus_tag="ACHOSM_RS03820"
                     /old_locus_tag="pgaptmp_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGU4557344.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17628..18281
                     /gene="ynjD"
                     /locus_tag="ACHOSM_RS03825"
                     /old_locus_tag="pgaptmp_000765"
     CDS             17628..18281
                     /gene="ynjD"
                     /locus_tag="ACHOSM_RS03825"
                     /old_locus_tag="pgaptmp_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU4557345.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            18348..19655
                     /gene="ynjE"
                     /locus_tag="ACHOSM_RS03830"
                     /old_locus_tag="pgaptmp_000766"
     CDS             18348..19655
                     /gene="ynjE"
                     /locus_tag="ACHOSM_RS03830"
                     /old_locus_tag="pgaptmp_000766"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HGU4557346.1"
                     /translation="MKRVSQMTALAMALGLTCASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLYEWIKQHNLKTDAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19664..20284)
                     /gene="ynjF"
                     /locus_tag="ACHOSM_RS03835"
                     /old_locus_tag="pgaptmp_000767"
     CDS             complement(19664..20284)
                     /gene="ynjF"
                     /locus_tag="ACHOSM_RS03835"
                     /old_locus_tag="pgaptmp_000767"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU4557347.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            20371..20778
                     /gene="nudG"
                     /locus_tag="ACHOSM_RS03840"
                     /old_locus_tag="pgaptmp_000768"
     CDS             20371..20778
                     /gene="nudG"
                     /locus_tag="ACHOSM_RS03840"
                     /old_locus_tag="pgaptmp_000768"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HGU4557348.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPRLEAFMALRAARAAD"
     gene            complement(20744..21016)
                     /gene="ynjH"
                     /locus_tag="ACHOSM_RS03845"
                     /old_locus_tag="pgaptmp_000769"
     CDS             complement(20744..21016)
                     /gene="ynjH"
                     /locus_tag="ACHOSM_RS03845"
                     /old_locus_tag="pgaptmp_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HGU4557349.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            21252..22595
                     /gene="gdhA"
                     /locus_tag="ACHOSM_RS03850"
                     /old_locus_tag="pgaptmp_000770"
     CDS             21252..22595
                     /gene="gdhA"
                     /locus_tag="ACHOSM_RS03850"
                     /old_locus_tag="pgaptmp_000770"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGU4557350.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(22712..23752)
                     /gene="ynjI"
                     /locus_tag="ACHOSM_RS03855"
                     /old_locus_tag="pgaptmp_000771"
     CDS             complement(22712..23752)
                     /gene="ynjI"
                     /locus_tag="ACHOSM_RS03855"
                     /old_locus_tag="pgaptmp_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4557351.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGG
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(23880..25841)
                     /gene="topB"
                     /locus_tag="ACHOSM_RS03860"
                     /old_locus_tag="pgaptmp_000772"
     CDS             complement(23880..25841)
                     /gene="topB"
                     /locus_tag="ACHOSM_RS03860"
                     /old_locus_tag="pgaptmp_000772"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HGU4557352.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDTVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGV
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(25846..26889)
                     /gene="selD"
                     /locus_tag="ACHOSM_RS03865"
                     /old_locus_tag="pgaptmp_000773"
     CDS             complement(25846..26889)
                     /gene="selD"
                     /locus_tag="ACHOSM_RS03865"
                     /old_locus_tag="pgaptmp_000773"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HGU4557353.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(27006..27557)
                     /gene="ydjA"
                     /locus_tag="ACHOSM_RS03870"
                     /old_locus_tag="pgaptmp_000774"
     CDS             complement(27006..27557)
                     /gene="ydjA"
                     /locus_tag="ACHOSM_RS03870"
                     /old_locus_tag="pgaptmp_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HGU4557354.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            27718..29574
                     /gene="sppA"
                     /locus_tag="ACHOSM_RS03875"
                     /old_locus_tag="pgaptmp_000775"
     CDS             27718..29574
                     /gene="sppA"
                     /locus_tag="ACHOSM_RS03875"
                     /old_locus_tag="pgaptmp_000775"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HGU4557355.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            29739..30755
                     /gene="ansA"
                     /locus_tag="ACHOSM_RS03880"
                     /old_locus_tag="pgaptmp_000776"
     CDS             29739..30755
                     /gene="ansA"
                     /locus_tag="ACHOSM_RS03880"
                     /old_locus_tag="pgaptmp_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGU4557356.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            30766..31407
                     /gene="pncA"
                     /locus_tag="ACHOSM_RS03885"
                     /old_locus_tag="pgaptmp_000777"
     CDS             30766..31407
                     /gene="pncA"
                     /locus_tag="ACHOSM_RS03885"
                     /old_locus_tag="pgaptmp_000777"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HGU4557357.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(31500..32858)
                     /gene="ydjE"
                     /locus_tag="ACHOSM_RS03890"
                     /old_locus_tag="pgaptmp_000778"
     CDS             complement(31500..32858)
                     /gene="ydjE"
                     /locus_tag="ACHOSM_RS03890"
                     /old_locus_tag="pgaptmp_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4557358.1"
                     /translation="MEQYDQIGARLDSLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEGAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLLLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(32976..33734)
                     /gene="ydjF"
                     /locus_tag="ACHOSM_RS03895"
                     /old_locus_tag="pgaptmp_000779"
     CDS             complement(32976..33734)
                     /gene="ydjF"
                     /locus_tag="ACHOSM_RS03895"
                     /old_locus_tag="pgaptmp_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4557359.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALMVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(33871..34851)
                     /gene="ydjG"
                     /locus_tag="ACHOSM_RS03900"
                     /old_locus_tag="pgaptmp_000780"
     CDS             complement(33871..34851)
                     /gene="ydjG"
                     /locus_tag="ACHOSM_RS03900"
                     /old_locus_tag="pgaptmp_000780"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HGU4557360.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(34861..35808)
                     /gene="ydjH"
                     /locus_tag="ACHOSM_RS03905"
                     /old_locus_tag="pgaptmp_000781"
     CDS             complement(34861..35808)
                     /gene="ydjH"
                     /locus_tag="ACHOSM_RS03905"
                     /old_locus_tag="pgaptmp_000781"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGU4557361.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFERFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNYAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(35813..36649)
                     /gene="ydjI"
                     /locus_tag="ACHOSM_RS03910"
                     /old_locus_tag="pgaptmp_000782"
     CDS             complement(35813..36649)
                     /gene="ydjI"
                     /locus_tag="ACHOSM_RS03910"
                     /old_locus_tag="pgaptmp_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HGU4557362.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMARKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(36670..37713)
                     /gene="ydjJ"
                     /locus_tag="ACHOSM_RS03915"
                     /old_locus_tag="pgaptmp_000783"
     CDS             complement(36670..37713)
                     /gene="ydjJ"
                     /locus_tag="ACHOSM_RS03915"
                     /old_locus_tag="pgaptmp_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HGU4557363.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAITVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(37730..39109)
                     /gene="ydjK"
                     /locus_tag="ACHOSM_RS03920"
                     /old_locus_tag="pgaptmp_000784"
     CDS             complement(37730..39109)
                     /gene="ydjK"
                     /locus_tag="ACHOSM_RS03920"
                     /old_locus_tag="pgaptmp_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4557364.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAVMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     AVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFSIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITSVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIVVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(39136..40212)
                     /gene="ydjL"
                     /locus_tag="ACHOSM_RS03925"
                     /old_locus_tag="pgaptmp_000785"
     CDS             complement(39136..40212)
                     /gene="ydjL"
                     /locus_tag="ACHOSM_RS03925"
                     /old_locus_tag="pgaptmp_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU4557365.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(40582..40854)
                     /gene="yeaC"
                     /locus_tag="ACHOSM_RS03930"
                     /old_locus_tag="pgaptmp_000786"
     CDS             complement(40582..40854)
                     /gene="yeaC"
                     /locus_tag="ACHOSM_RS03930"
                     /old_locus_tag="pgaptmp_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HGU4557366.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(40896..41309)
                     /gene="msrB"
                     /locus_tag="ACHOSM_RS03935"
                     /old_locus_tag="pgaptmp_000787"
     CDS             complement(40896..41309)
                     /gene="msrB"
                     /locus_tag="ACHOSM_RS03935"
                     /old_locus_tag="pgaptmp_000787"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGU4557367.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            41651..42646
                     /gene="gapA"
                     /locus_tag="ACHOSM_RS03940"
                     /old_locus_tag="pgaptmp_000788"
     CDS             41651..42646
                     /gene="gapA"
                     /locus_tag="ACHOSM_RS03940"
                     /old_locus_tag="pgaptmp_000788"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU4557368.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            42730..43614
                     /gene="yeaD"
                     /locus_tag="ACHOSM_RS03945"
                     /old_locus_tag="pgaptmp_000789"
     CDS             42730..43614
                     /gene="yeaD"
                     /locus_tag="ACHOSM_RS03945"
                     /old_locus_tag="pgaptmp_000789"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HGU4557369.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVNVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(43665..44519)
                     /gene="yeaE"
                     /locus_tag="ACHOSM_RS03950"
                     /old_locus_tag="pgaptmp_000790"
     CDS             complement(43665..44519)
                     /gene="yeaE"
                     /locus_tag="ACHOSM_RS03950"
                     /old_locus_tag="pgaptmp_000790"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HGU4557370.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMSVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHHGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(44609..45355)
                     /gene="mipA"
                     /locus_tag="ACHOSM_RS03955"
                     /old_locus_tag="pgaptmp_000791"
     CDS             complement(44609..45355)
                     /gene="mipA"
                     /locus_tag="ACHOSM_RS03955"
                     /old_locus_tag="pgaptmp_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HGU4557371.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            45791..47725
                     /gene="yeaG"
                     /locus_tag="ACHOSM_RS03960"
                     /old_locus_tag="pgaptmp_000792"
     CDS             45791..47725
                     /gene="yeaG"
                     /locus_tag="ACHOSM_RS03960"
                     /old_locus_tag="pgaptmp_000792"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HGU4557372.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQRKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            47838..49121
                     /gene="yeaH"
                     /locus_tag="ACHOSM_RS03965"
                     /old_locus_tag="pgaptmp_000793"
     CDS             47838..49121
                     /gene="yeaH"
                     /locus_tag="ACHOSM_RS03965"
                     /old_locus_tag="pgaptmp_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HGU4557373.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            49268..50743
                     /gene="cdgI"
                     /locus_tag="ACHOSM_RS03970"
                     /old_locus_tag="pgaptmp_000794"
     CDS             49268..50743
                     /gene="cdgI"
                     /locus_tag="ACHOSM_RS03970"
                     /old_locus_tag="pgaptmp_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGU4557374.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPILILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            50924..52414
                     /gene="dgcJ"
                     /locus_tag="ACHOSM_RS03975"
                     /old_locus_tag="pgaptmp_000795"
     CDS             50924..52414
                     /gene="dgcJ"
                     /locus_tag="ACHOSM_RS03975"
                     /old_locus_tag="pgaptmp_000795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HGU4557375.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRTICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            52457..52960
                     /gene="yeaK"
                     /locus_tag="ACHOSM_RS03980"
                     /old_locus_tag="pgaptmp_000796"
     CDS             52457..52960
                     /gene="yeaK"
                     /locus_tag="ACHOSM_RS03980"
                     /old_locus_tag="pgaptmp_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HGU4557376.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(52961..53065)
                     /gene="yoaI"
                     /locus_tag="ACHOSM_RS03985"
                     /old_locus_tag="pgaptmp_000797"
     CDS             complement(52961..53065)
                     /gene="yoaI"
                     /locus_tag="ACHOSM_RS03985"
                     /old_locus_tag="pgaptmp_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HGU4557377.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            53235..53681
                     /gene="yeaL"
                     /locus_tag="ACHOSM_RS03990"
                     /old_locus_tag="pgaptmp_000798"
     CDS             53235..53681
                     /gene="yeaL"
                     /locus_tag="ACHOSM_RS03990"
                     /old_locus_tag="pgaptmp_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HGU4557378.1"
                     /translation="MFDATLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(53638..54459)
                     /gene="nimR"
                     /locus_tag="ACHOSM_RS03995"
                     /old_locus_tag="pgaptmp_000799"
     CDS             complement(53638..54459)
                     /gene="nimR"
                     /locus_tag="ACHOSM_RS03995"
                     /old_locus_tag="pgaptmp_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HGU4557379.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHQPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPNPRQ"
     gene            54556..55737
                     /gene="nimT"
                     /locus_tag="ACHOSM_RS04000"
                     /old_locus_tag="pgaptmp_000800"
     CDS             54556..55737
                     /gene="nimT"
                     /locus_tag="ACHOSM_RS04000"
                     /old_locus_tag="pgaptmp_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HGU4557380.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            55792..56139
                     /gene="yeaO"
                     /locus_tag="ACHOSM_RS04005"
                     /old_locus_tag="pgaptmp_000801"
     CDS             55792..56139
                     /gene="yeaO"
                     /locus_tag="ACHOSM_RS04005"
                     /old_locus_tag="pgaptmp_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HGU4557381.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(56161..56415)
                     /gene="yoaF"
                     /locus_tag="ACHOSM_RS04010"
                     /old_locus_tag="pgaptmp_000802"
     CDS             complement(56161..56415)
                     /gene="yoaF"
                     /locus_tag="ACHOSM_RS04010"
                     /old_locus_tag="pgaptmp_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HGU4557382.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            56598..57623
                     /gene="dgcP"
                     /locus_tag="ACHOSM_RS04015"
                     /old_locus_tag="pgaptmp_000803"
     CDS             56598..57623
                     /gene="dgcP"
                     /locus_tag="ACHOSM_RS04015"
                     /old_locus_tag="pgaptmp_000803"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HGU4557383.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            57656..57754
                     /gene="yoaK"
                     /locus_tag="ACHOSM_RS04020"
                     /old_locus_tag="pgaptmp_000804"
     CDS             57656..57754
                     /gene="yoaK"
                     /locus_tag="ACHOSM_RS04020"
                     /old_locus_tag="pgaptmp_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HGU4557384.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            57757..57831
                     /gene="yoaJ"
                     /locus_tag="ACHOSM_RS04025"
                     /old_locus_tag="pgaptmp_000805"
     CDS             57757..57831
                     /gene="yoaJ"
                     /locus_tag="ACHOSM_RS04025"
                     /old_locus_tag="pgaptmp_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HGU4557385.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(57890..58138)
                     /gene="yeaQ"
                     /locus_tag="ACHOSM_RS04030"
                     /old_locus_tag="pgaptmp_000806"
     CDS             complement(57890..58138)
                     /gene="yeaQ"
                     /locus_tag="ACHOSM_RS04030"
                     /old_locus_tag="pgaptmp_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGU4557386.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(58286..58468)
                     /gene="yoaG"
                     /locus_tag="ACHOSM_RS04035"
                     /old_locus_tag="pgaptmp_000807"
     CDS             complement(58286..58468)
                     /gene="yoaG"
                     /locus_tag="ACHOSM_RS04035"
                     /old_locus_tag="pgaptmp_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HGU4557387.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(58499..58831)
                     /gene="yeaR"
                     /locus_tag="ACHOSM_RS04040"
                     /old_locus_tag="pgaptmp_000808"
     CDS             complement(58499..58831)
                     /gene="yeaR"
                     /locus_tag="ACHOSM_RS04040"
                     /old_locus_tag="pgaptmp_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HGU4557388.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQ"
     gene            complement(59004..59642)
                     /gene="leuE"
                     /locus_tag="ACHOSM_RS04045"
                     /old_locus_tag="pgaptmp_000809"
     CDS             complement(59004..59642)
                     /gene="leuE"
                     /locus_tag="ACHOSM_RS04045"
                     /old_locus_tag="pgaptmp_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HGU4557389.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(59769..60692)
                     /gene="dmlR"
                     /locus_tag="ACHOSM_RS04050"
                     /old_locus_tag="pgaptmp_000810"
                     /pseudo
     CDS             complement(59769..60692)
                     /gene="dmlR"
                     /locus_tag="ACHOSM_RS04050"
                     /old_locus_tag="pgaptmp_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
     gene            60795..61880
                     /gene="dmlA"
                     /locus_tag="ACHOSM_RS04055"
                     /old_locus_tag="pgaptmp_000811"
     CDS             60795..61880
                     /gene="dmlA"
                     /locus_tag="ACHOSM_RS04055"
                     /old_locus_tag="pgaptmp_000811"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HGU4557390.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILPDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            62131..63738
                     /gene="yeaV"
                     /locus_tag="ACHOSM_RS04060"
                     /old_locus_tag="pgaptmp_000812"
     CDS             62131..63738
                     /gene="yeaV"
                     /locus_tag="ACHOSM_RS04060"
                     /old_locus_tag="pgaptmp_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HGU4557391.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADVANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFHV
                     RKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSAL
                     TGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPTE
                     FITNSIINAIGLTTQNFLQMSLFTDPLGDCSFTRNWTVFYWLWWISYTPGVAMFVTRV
                     SRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQQ
                     VLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWCV
                     VITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQVE
                     HYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            63770..64894
                     /gene="yeaW"
                     /locus_tag="ACHOSM_RS04065"
                     /old_locus_tag="pgaptmp_000813"
     CDS             63770..64894
                     /gene="yeaW"
                     /locus_tag="ACHOSM_RS04065"
                     /old_locus_tag="pgaptmp_000813"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HGU4557392.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            64950..65915
                     /gene="yeaX"
                     /locus_tag="ACHOSM_RS04070"
                     /old_locus_tag="pgaptmp_000814"
     CDS             64950..65915
                     /gene="yeaX"
                     /locus_tag="ACHOSM_RS04070"
                     /old_locus_tag="pgaptmp_000814"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HGU4557393.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(65969..67084)
                     /gene="rnd"
                     /locus_tag="ACHOSM_RS04075"
                     /old_locus_tag="pgaptmp_000815"
     CDS             complement(65969..67084)
                     /gene="rnd"
                     /locus_tag="ACHOSM_RS04075"
                     /old_locus_tag="pgaptmp_000815"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HGU4557394.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEDSGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(67166..68851)
                     /gene="fadD"
                     /locus_tag="ACHOSM_RS04080"
                     /old_locus_tag="pgaptmp_000816"
     CDS             complement(67166..68851)
                     /gene="fadD"
                     /locus_tag="ACHOSM_RS04080"
                     /old_locus_tag="pgaptmp_000816"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HGU4557395.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFCKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(69056..69637)
                     /gene="yeaY"
                     /locus_tag="ACHOSM_RS04085"
                     /old_locus_tag="pgaptmp_000817"
     CDS             complement(69056..69637)
                     /gene="yeaY"
                     /locus_tag="ACHOSM_RS04085"
                     /old_locus_tag="pgaptmp_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HGU4557396.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(69677..70372)
                     /gene="tsaB"
                     /locus_tag="ACHOSM_RS04090"
                     /old_locus_tag="pgaptmp_000818"
     CDS             complement(69677..70372)
                     /gene="tsaB"
                     /locus_tag="ACHOSM_RS04090"
                     /old_locus_tag="pgaptmp_000818"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGU4557397.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPEIVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(70430..72340)
                     /gene="yoaA"
                     /locus_tag="ACHOSM_RS04095"
                     /old_locus_tag="pgaptmp_000819"
     CDS             complement(70430..72340)
                     /gene="yoaA"
                     /locus_tag="ACHOSM_RS04095"
                     /old_locus_tag="pgaptmp_000819"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HGU4557398.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLLVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIAQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            72472..72816
                     /gene="yoaB"
                     /locus_tag="ACHOSM_RS04100"
                     /old_locus_tag="pgaptmp_000820"
     CDS             72472..72816
                     /gene="yoaB"
                     /locus_tag="ACHOSM_RS04100"
                     /old_locus_tag="pgaptmp_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU4557399.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT        18084 a        18818 c        17663 g        18327 t
ORIGIN      
        1 aagccatttt tgattgcatt aacgtgtggc tgctttccta ttttaactgc aaaagggagg
       61 agtacctttt caagcgatgc aataacatta ctcatagaaa aataccctta aaaaccgcaa
      121 tttaaatatt gcggtattga tttatgaaat aactctttga cgggaagatt taaaataaat
      181 taatttgctg cggctttttt aatcgttgca accgcagcct taagcacgcc taaaccatcg
      241 actttgccat aaagcagcga gtcaattact tcaaccggtt tgttgggtaa caaacgctgg
      301 atttcgggca acatataagc aatctgcggc cctaataaca cgacatcggc attctgacct
      361 ttttcaccag ccagtgtttc cggaaatgct tcaataatga ccggaacttc atatttttct
      421 gcctgtgcgc gcatttttga taccagtaaa gaggtagaca tgcccgcaga acaaaacaga
      481 taaatgtgtt tcttttccat aaaactgccc tcatcgacga ttatctgtca gccagacact
      541 ccgcaagcct taacctgctt ccatgctctg ggtaacttgc gaaaccaaca taactttttt
      601 tgtggctgaa aggagtatac ggtatagacc tgagcaatag tatttctttg accttataaa
      661 ttgtttctcc ttgacctgtg gttatgaccc tcttcacatt tcgggcaaat aattcgcgaa
      721 gataattaag aactcaggcc aaaaaaaacc ggcgcaatgg ccggtttccg ttgttactca
      781 agctttcaga cgaattgatt acttcgcagc ctgtggatca gtgtcgtatt cagcacaggt
      841 ctgataaccg gagttgatga catgtccggt atcatctaac gcgacaaagt aggtttctgc
      901 tttaccatca cgttgaccca ggatgtaggt ctggcaagta ccgcgagcat ggatcatgct
      961 cacttcagac gaaggtttac ccgcaatttg tgcaacctgc gcccggctca tgcctttttt
     1021 gacgtctttc accacaggct gtacaaactg gtctttggta cgatcataag ccgtacaacc
     1081 cgccagcatg gttaataccg ccgctgcact cagaattcct gccatattct tgttcatatt
     1141 ccgtcctctt gtttatcagc gtgttagata agcctggaat acattgggcg ctttttcaag
     1201 cccgtgaacg aaacggctcc gctttcagag gattcctgta tgacgtttta accaccattc
     1261 agcccgctgt cgcttgtcgt ttcagtagca acgggttagc tttaaggaag ttttgtcttt
     1321 tctgtctgga ggggttcaat gacattgcaa caacaaataa taaaggcgct gggcgcaaaa
     1381 ccgcagatta atgctgaaga ggaaattcgt cgtagtgtcg attttctgaa aagctacctg
     1441 caaacttatc cgttcattaa atcactggtg ctcgggatca gcggcggtca ggactccacg
     1501 cttgccggaa agctgtgcca gatggcgatt aatgagctgc gccaggaaac tggcaacgaa
     1561 tcactgcaat ttattgccgt acgcctgccc tatggtgttc aggccgacga acaagattgc
     1621 caggatgcca ttgcctttat tcaaccggat cgcgtattaa ccgttaatat caagggcgcg
     1681 gtattggcca gcgaacaggc attgcgggaa gcaggcattg aactgagcga ttttgtccgt
     1741 ggcaatgaaa aagcgcgtga gcggatgaaa gcacaatata gcattgcggg tatgaccagc
     1801 ggtgtcgtgg tgggcaccga tcatgcagca gaagccatta ccggattctt cactaaatat
     1861 ggtgacggcg gtacagacat taatccgctg tatcgtctca acaaacgtca gggtaaacag
     1921 ttactggcgg cattaggttg cccggaacac ctttataaga aagcgccaac ggccgatctg
     1981 gaagatgatc gcccttctct gccagatgaa gtggcactcg gcgtgaccta tgacaatatc
     2041 gacgactatc tggaagggaa aaacgtacct gaacaggtcg ccagaacaat agagaactgg
     2101 tatctgaaaa ccgaacataa acgccgtccg ccaattaccg ttttcgatga tttctggaaa
     2161 aagtaataat ttgcacataa tggtttgtgc gaaaaagagt aatttgttca cgccggatgc
     2221 ggcgtgaacg ccttattcga cctacaaaac tatgcaaatt caatatattg caggagtcga
     2281 ataggcctga caggcgtagc acgtcagacg gtgtaacctt tgtcatcgac ccgcctcttt
     2341 tttaatcgct ccccgcctgt tacactggat agataaccag cattcggagt caacagtggt
     2401 acggcgttta acttctccgc ggctcgaatt tgaagctgcg gcaatttatg aatatcccga
     2461 acatttacgt tcattcctta atgacttacc cacccgacca ggggtgtatc tgtttcatgg
     2521 cgaaagtgac accatgccgc tctatatcgg caaaagcgtt aatatccgca gtcgggtgct
     2581 ttctcattta cgcaccccgg atgaagccgc catgctacgg caatcccggc gaattagctg
     2641 gatctgcacc gcaggcgaaa tcggcgctct gctccttgaa gcgcgattaa tcaaagaaca
     2701 acagccgctg tttaataaac ggttgcgccg caatcgccag ctctgtgccc tgcaattaaa
     2761 tgaaaagcgc gtcgatgtgg tgtatgccaa agaggtggat ttttcacgag cccccaacct
     2821 gtttggcctg tttgccaata ggcgcgcagc tttgcaagca ttgcagagca tcgctgatga
     2881 acaaaaactt tgttatggcc tgttgggact ggaaccgtta agtcgcggtc gtgcatgttt
     2941 tcgttcagcg ctaaaacgtt gcgccggagc atgctgcggt aaagagagcc atgaggaaca
     3001 tgcgctacgc ttgcgccagt ctctggagcg tttgcgggtg gtgtgttggc cttggcaagg
     3061 tgcggtggca ctgaaagaac agcacccgga aatgactcaa tatcatatta ttcaaaactg
     3121 gctgtggctg ggggcggtta attcgctgga agaagcgaca acgttaattc ggacacccgc
     3181 cgggtttgat cacgacggtt ataaaattct ttgtaagccg ctgctttccg gtaactatga
     3241 aattactgaa cttgatccgg cgaatgacca gcgagccagt tgatttcact gaacaacagt
     3301 ttaccttgcg gttgtaaaag ccgcagggtg tgcctgccat cgaaccagca cgcgccctgg
     3361 tcggtagtga gcaatttatc gcccagttgc cacgccccgt taatcacaaa caccaccccg
     3421 ccgcgtgagc caaaggtagt aaacgtacgt tcggcaattc tgactttcgc cttacagaca
     3481 tccaggcgtg tcatgatgtt gaaatccatc gatatctgcc ctgccgtcag tttcgctttt
     3541 accacctggt ccgctgcaaa ggcaaaaggc tgtaacggtt ttaaggtatg gttaaagcgg
     3601 tctgcgcttt caaggagcat ctcgccgcct tccagcaacg tcactatcct ttccatgccg
     3661 ggaaacaaag aaaactcccc attcgccgcg atggatgcaa tgctggcacg ccagtaaaaa
     3721 tcacgttttg ccggtgggaa agtgcaaatt tcgcgcgttt caccggcagc atttcgccac
     3781 agattcaccg acattttacg catatcaaag tattccatac tcgctcccgg cccgttcttt
     3841 tcattgttat cgttcaggta accgtggatg gttatcggca actcgggtga tttacgttag
     3901 tggtgatcag gcttttttta caactgtttg agaagagaaa agaaaaccgc cgatcctgtc
     3961 caccgcatta ctgcaaggta gtggacaaga ccggcggtct taagtttttt ggctgaaaga
     4021 ttattcagca gttgcaggca ttttaccttt tgccgctgga cgttctgtca gacgcttctc
     4081 aaaattagca ttaaattgct ttttctgttc cggcgtcagg atgttgtaaa ttttgttctg
     4141 ggtttccatg tgcgccagca tgttagcttt gcgctgttct tccatttttg cgatctgcgc
     4201 ttcagctttt gctttatcga aggtatcgct ggcaatgatg tcatgcattg cgcggcgttc
     4261 ttccagcggc ggacgtttca tctggtcacg ctggcctttc atgatttcgc ggatctgctg
     4321 tttctgcgcg tcggtcaggt tcaggtcttt gaacatcatg tcctgatgcg gaccgaactt
     4381 gcctttgtgg tgcatcatcg gcttcgcgtc agccggtgct gcggtagtgg tgtctgcggc
     4441 atgggccagg ttagccgcgc caagagccag ggtagaggca acaaacagtg cagttaattt
     4501 acgcatattc tatatccttc ctttcagtta tttattacgg ctttcttaag tagcgtgccg
     4561 tgttgacgag attaacttta ctgactttag cgtcaattaa tcagagcaac ggtaaaacaa
     4621 tgaaagtgta aaaaacactt ttgcgccaat tatggagaaa aaaagaaaat ttgatggaga
     4681 gtgatgagag aatattacaa cacgatgatt ttgcagagat tatgaagaac tataccggat
     4741 gactggtgat aaataaagca aataaccagg attaatctgt attaatttat aagaaagcaa
     4801 cttaataccc gcagaatgat ttctgcgggt aagtattagc ttattttttc gagcattaat
     4861 cccgcgcgta atcccaacgc taccaacgga ttagggaata acacatactc tacatcatgg
     4921 gttacggtaa aacgttcctc tccgtcctgc gccagcaatg ttcctttctc aaacggcata
     4981 aaattcagcg tgtcacttgc catatgcatt tcgaaggacg gcgagtggcg agtaatttgc
     5041 gaaaccaccc gataacggag cggcggtgtt ctcacgatac cgacactctc accagatagc
     5101 agcgcagcaa ttgcgctggc agttactgca aactggcgaa gatcgttttg cccaaagggc
     5161 aacgctttgc caagttccag cgtacaggcc agcgcgccaa aatgtctggc gctgaaatgg
     5221 gtaaacgtac caccaggttc ctgatggaac accagcgcct ccagccccgc cgcacccagc
     5281 cacgtcagaa atttctcgtc ccagggaatg tcgcgttgcg gtaatacacc gaactgcgga
     5341 tgcaaggagc cacgaattgc ggtatgtaga tcaaggtgcc agcgcacaga ttctttgccc
     5401 tggtcataaa aatcttccag gcactgttcc agttcgcgcg cccgacaggt ttctccgctt
     5461 tcagcaaata gctgccaacg accgccaaac attcgattca tatcgctatg gcaataacgt
     5521 ttcccttgct tcagcgcagg aggattcccg aggatcacca gcaaccgcca acgtaacggg
     5581 atctcgccgt gagatatcgc gccaagcaac gcgtccaaca tctccacagg tgccgtctca
     5641 ttaccgtgta ttcccgctga aatcaccagt gcgccttgcg gtggcgttaa tggcgtcagt
     5701 tccagcacac catcgcccag ccagcgccag cgaacgccgt tgatttctcg ctcggtgata
     5761 accggttttt tacccgttaa ggtcagagca agaaaattat ccattgcccc ctccctcgcg
     5821 ctggaacgga taaaccgaac cgagattcag taattggctc aatacatcca gtgcttcccg
     5881 cccttcgcgc agcaattgcg ggtcggccag atcggcagca gtaaggcgat cgcggtagta
     5941 acgatccacc cagtcattga gcgcattaaa cagcgtatcg ttcatcatca ccgccggatt
     6001 caccgcccgg cgttcttctt ctgtcaatac cacccgcaac cgcaggcacg ccgggccgcc
     6061 gccattcgcc atgctttcac gtaaatcaaa gacttttagt tcgctaatcg ggttgtcagc
     6121 ggcaaggagt tcattgagat aaccccatac tccggcgtgt tcccgacact cctgaggcag
     6181 caccaacatc atggaaccat catcgcggct caacagttgg ctgttaaaca gataggtaga
     6241 caccgcatcc gacacggaaa cctgagttgc cggaacttct atcgccataa aaccgttcac
     6301 ccgcgcacgc aggtttgcca gtaactgtga ctggcgagcg aacgcctgtt ggtggcaaaa
     6361 cagcacctgg cggttactca cggcaatcac gtcattatga aaaacgccct ggtcgataac
     6421 gtccgggttt tgctgggcga aaatcacctg ttggggattc acctgattca gccttgccac
     6481 cgcctcgctg gcttcgcgag tctgtcgcgc cggataacgg gaaggccggg tatcattgcc
     6541 ttcttctcgc ccgtagacaa aaagttgcat acccggctca ccgtaatgac cgccgagacg
     6601 attgtggttt gccgccccct catcaccgag caacgctacc tgcggcaacg ccgaatgaac
     6661 gctaaatttc tcttcgtcgt taaaaatcgc ttttaaaagt gattcggtga cgtgcgcttc
     6721 cagcgagcgg tgaaatttat tgttcaggtt ggcaacggtg agatgcactt tgccatccag
     6781 cgtatcggca gatggcgcga tcgttgccgc attggctacc cacattggcg aagcggaact
     6841 gacgctggaa agccagtgcg gtgcctggcg tgcaactttt tccagtacct gctcatcgct
     6901 accactgaat cccaactgac gcagcaccgg aataaacgga cgctcgtgcg gcgggatcac
     6961 ggcctggggg aatcccgcat cggcaagggc tttcattttc agtaagccct gttttgccgc
     7021 ctgtcgcggg ttagacacct gaaaacggtg acgggtagag gcttcattac caaacgacag
     7081 gccagcgtaa tgatgcgtca gccctaccag cccgtcgaaa ttaacttccc aggcgttcat
     7141 cgcaccacct catcggaaaa atccagcccg gggttaagcg ttgcgggcaa agttaacgag
     7201 tccgactcca ggctcgccat aggccatgcg cagtaatctg cggcatacca ggcgctgggg
     7261 cgatggttac cggatgcacc aatgccgccg aatggcgcgg tactggcagc accagtaagc
     7321 ggtttgttcc agttaacaat ccccgcccgc gcctccagca acagttgatc gaatttttcc
     7381 cgctcggggg aaaccagacc gcaagagagt ccgaagcgag tgttattcgc cattagaatc
     7441 gcttcctcga aagaatcata acgccagacg cgcaataacg gtccgaacac ctcttcatct
     7501 ggtacgccag caacgcctgt catttcaatg atccccggcg tcagcaacga tgtctctgat
     7561 tgtaataagc gcggcgcaag tagggttcgt ccgcccatcg cttccagttg ctgccaggca
     7621 gtaaccacct gctgtgcggc ctgttcagaa atcagcccgc caataaacgg ctgcggttcg
     7681 tcatcccagt tgcccggcgt taatcgctgg ctgacggcaa ccagacgagc aagaaacgca
     7741 tcgccctgcg ctccgctttt gagcaataaa cggcgggcgc aggtgcagcg ttgcccggct
     7801 gtgacaaacg ccgactgaat ggtcagatgg acagccgcgt cgatatccgc cacctcatcg
     7861 ataattagcg gattattacc gcccatctca agggcgagaa ttttctccgg ctgaccggag
     7921 agctggcgat gcagctggta gccggtattg gcgctaccgg taaacagcaa accgtcgaga
     7981 tcctccagcg cactcagcgc ctgacccgtt tcacgcccgc cctgcaccag gttcagtacg
     8041 cccggcggca agccagcctg ctgccataaa cgcattaccg cttcgccact ccacggtgtc
     8101 agttcgctgg gtttaaagat aatggtgtta cctgccagca atgccggaac gatatgtccg
     8161 ttcggcaaat gaccagggaa attatacggc ccaaacaccg ccagcacgcc gtgcgggcga
     8221 tgtcgcaggc tcgccgcacc gtccggcatt tcactacgct gctcgccggt acgaacgtga
     8281 tacgccttaa ttgatatcgc gattttattg atcatcgccg tcacttcggt tgccgcttcc
     8341 cagcgcggct tacccgtttc tctggcaata atcgcggtta attcggcttt attgctttcc
     8401 agcaatccgg caaagcgttc gacaacgacc tgacgttcag ccaatgagag ccgcgcccag
     8461 cgcggaaacg ccgcacgggc tgcccgacaa gcctgttcga cctgagcggc atcggcatca
     8521 ttgccttgcc atagcacctc gcccgatacc ggattacgct tcacacgcga tgcgccctgg
     8581 cccgttatcc agtcaccgtt aatccataaa gtcatgctgt tttctcctct gcgcacaggc
     8641 gcaccagacg aacgcgatcc cctgcgtggc atttgagggc atccagttgt gcggcggtta
     8701 aaatcaaacg ctcggttgcc ggatcggtac gcgccagcac cacgcggaaa tggtgataat
     8761 tttcattggc gaccaggcag gccggaaaat cgccctgcgc aggctgccct tctgccactt
     8821 ccaccagccg acttttacgg atggcgcgca cgcggtcgat gtcacactca agcgttggcc
     8881 cgccgtcgaa gatgtcgata tagttgcggt aacgaaaacc ttctttctcc agcaccgcgc
     8941 gggcaggcgc ggtttgcgga tgtacctgac cgatgacgtc ccgggcttcc tgggataaaa
     9001 agtgggtata gatcggatgt ttcggcatca gttctgcaat aaatgccttt tgcccggtgc
     9061 cgcagagaaa atcggcgcgg ctaaaatcca tcgaaaagaa gcgtttaccg aggctttgcc
     9121 agaacggtga atagccgtgt tcgtcaatca ccccgcgcat ttcagcaacc actttgtcat
     9181 taaacttgtc gcgaaaagcc gccataaaca taaagcgcga tttcgacagc aaatagccgt
     9241 tgccctcttt gcgccagtcc gggtcgagaa acagcgtgca cagctcgctg ctgccggtgt
     9301 gatcgttact aagaaacagc gtcggcaatg cgttatagac attcagctct tttgaggcgt
     9361 gaaccaacgt gccgacgcga tagttgtacc agggatcgtt cagcccaacc gccacctcaa
     9421 tggcacaaat ccccgccacg gtgcctgtct cgctatcttc cagcacgaac acatagccct
     9481 gctcactttt gggcagttcg ccttgccagg ttttgattgc cctttcgata cgcgccgaaa
     9541 gcgtggcttc attggcggga agcgacgtca ggccgccgcc cgttttgctg gcaagctgca
     9601 tcagcgccga gacatctgat cgctcaacgg gacggatgac catcatgatg aacctcggct
     9661 aacaaagtgt tcgcaagcag ctgcaaagcg atccagtccg gtcgtcacct cttcttcgct
     9721 gacattgagc gcaggcgcaa aacgcaccac gttgccaccc gcaatcagta ccatcacgcc
     9781 tgctttcgcc gcttcctgag agatctgttt cgcttgcccg gcgtaatcgg cattcagtac
     9841 acagccaatc agcaaaccta agccgcgaac ttcactgaac aaaccatagc ggtgattaat
     9901 agtattaaga cgctcaacaa accagtcgtg acgctgttta acgccattaa gcatctctgg
     9961 tgtgttgatg agctccagca ctttgcctgc caccgccgag gccagcggat taccgccata
    10021 ggtggtgcca tgagtgccaa cggtcatcac gcgggcgcac tcttcggttg ccaacaacgc
    10081 accgaccggg aaaccgccgc ccagcgcttt ggcggtagtt aacagatcag gcgtcacgcc
    10141 gtagtgcata taggcataca gttccccggt gcgcccgacg ccggtttgta cttcatcaaa
    10201 aatcaacagc gcattgtggc ggttacacaa ttcacgcaga ccttgtaaaa acgcgttgct
    10261 ggctggcacc acaccgcctt ccccctggat gggttcgaca atcaccgcac aggtagagtc
    10321 gtcaatcagc gcgctggcag agttaatatc gttatatgca gcatgacgaa tatccgccgg
    10381 cagtggcgca aaatcctgtg aataggctgg ctgcccaccc gcactgacag taaacagcgt
    10441 gcgaccatga aacgcatttt tgaacgccac gatgccgctc ttatggctgc cgtagcggtc
    10501 gtgagcgaat ttacgcgcca gttttagcgc cgcttcgttg gcttccgcac cggagttaca
    10561 aaagaagacg cgatcggcaa acgtggcgtc gatcaatttt ttcgccagtc gcagtaccgg
    10621 ctcgttggtg taaccgttgc cggtatgcca gaacttactc gcctgttcgt tcagcgcttc
    10681 acgcagttcc ggatgcgcat ggcccagcgc gttcaccgca atgccacccg cgaagtcgat
    10741 atactctttc ccctgctgat cccacaagcg cgaaccttcg ccacgtaccg gtataaaggg
    10801 tgccggagcg taaacaggta tcatccattc atcaaagttt tcacgcgtaa ttggctgaga
    10861 catagcgacc tctacagtaa ataattcgtt atttatatgt taacaataag taatgtttgc
    10921 gctgtaaatg tagattgcag ggttcgtgcc agccagtgat aaaagtgcat aaacggcgga
    10981 ggctaactgg aaatcaagga gttataacca aaccatatgc atttaaagtg catataaagt
    11041 gaatacgttt gcgatgtgtg tgaataaaaa gaataaaaaa cgcaatgtta tgcagaagta
    11101 aaatataatt ctggaattgt gatcattgac gaaatttact ggaaattact gcgccattct
    11161 gacgcagcgc gcaccaaaag cgggcatttt ttgcgccatc gttgacatca ttaacaacca
    11221 tcgatcaaat cacttaacaa caggcggtaa gcaacgcgaa attctgctac catccacgca
    11281 ctctttatct gaataaatgg cagcgactat gaaatttgtc tcttttaata tcaacggcct
    11341 gcgcgccaga cctcaccagc ttgaagccat cgtcgaaaag caccaaccgg atgtgattgg
    11401 cctgcaggag acaaaagttc atgacgatat gtttccgctc gaagaggtgg cgaagctcgg
    11461 ctacaacgtg ttttatcacg ggcagaaagg ccattatggc gtggcgctgc tgaccaaaga
    11521 gacgccgatt gccgtgcgtc gcggctttcc cggtgacgac gaagaggcgc agcggcggat
    11581 tattatggcg gaaatcccct cactgctggg taatgtcacc gtgatcaacg gttacttccc
    11641 gcagggtgaa agccgcgacc atccgataaa attcccggca aaagcgcagt tttatcagaa
    11701 tctgcaaaac tacctggaaa ccgaactcaa acgtgataat ccggtactga ttatgggcga
    11761 tatgaatatc agccctacag atctggatat cggcattggc gaagaaaacc gtaagcgctg
    11821 gctgcgtacc ggtaaatgct ctttcctgcc ggaagagcgc gaatggatgg acaggctgat
    11881 gagctggggg ttggtcgata ccttccgcca tgctaatccg caaacagcag atcgtttctc
    11941 atggtttgat taccgctcaa aaggttttga cgataaccgt ggtctgcgca tcgacctgct
    12001 gctcgccagc caaccgctgg cagaatgttg catagaaacc ggcatcgact atgaaattcg
    12061 cagcatggaa aaaccgtccg atcacgcccc cgtctgggcg accttccgcc gctaatttag
    12121 cagctctcct ggctcaaact gggtcaggag aattaacctt gagaaaaatc aacaaactgt
    12181 cagtaatgat ttgttgcctg ccgtcctttg ttataccgtc actgcgtttt tagttgtctg
    12241 accacttctc tattatcaag tttgatatag gaaactccac gatgaacgct gagcgtaact
    12301 ttctgtttgc ctgtcttatt tttgcgctgg ccatttacgc tatccacgct ttcggtttat
    12361 tcgatctgct caccgattta ccccacttac agacactcat tcgccagagc ggattgttcg
    12421 gttatagcct ctatattctg ttattcatca ttgccaccct ctttctgtta ccaggaagca
    12481 tactggtgat cgctggagga atagtttttg gcccgctctt agggacacta ctctcattaa
    12541 ttgccgcaac gctggcctcc tcgtgctcat tcctgctggc gcgctggctg ggacgtgatt
    12601 tactgctgaa atacgtgggc catagccata ccttccaggc cattgaaaaa ggcattgcgc
    12661 gtaacggtat tgattttctt attctgaccc gcttaatccc gttgtttcct tacaatattc
    12721 aaaattacgc ttacggatta accaccatcg ccttctggcc ttataccctt atttcggcac
    12781 tcacgaccct gcccggtatt gttatttata ccgtgatggc aagcgatctc gccaatgaag
    12841 gcattacgct gcgctttatt ttacaactct gtctggcggg cctggcgctg tttattctcg
    12901 tccagctcgc aaaactctac gcccgacaca aacatgtgga tctgtctgct tcgcgccgca
    12961 gcccacttac tcacccaaaa aatgaaggat agaacgatgt cgcaacatta ttcagtgtca
    13021 tggaaaaaag gactggctgc actctgctta ctggctgttg ccgggcttag cggctgcgat
    13081 caaaaagaga atgcggcggc aaaagtggaa tacgacggac tttcgaacag ccaaccgctg
    13141 cgcgtcgatg ccaataacca tacggtaacc atgctggtgc aaattaatgg tcgtttcctc
    13201 accgacgaca ctcgtcacgg tattgtgttt aaagatggct ccaacggaca taaatcgctg
    13261 tttatggctt atgcgacccc gaaagcattt tatgaagccc tgaaagaggc aggtggtacg
    13321 ccgggcgaaa acatgacgat ggataataaa gaaacgactc atgtcacagg cagcaaactg
    13381 gatatttcgg tcaactggca aggggcggca aaagcgtatt ccttcgatga agtgattgtt
    13441 gacagtaatg gcaagaaact ggacatgcgc tttggcggta atttaacggc agcagaagag
    13501 aagaaaacgg gttgcctggt gtgtctggat agctgcccgg tcggcatcgt cagcaatgca
    13561 acatacactt atggtgcggt ggaaaaacgc ggcgaagtga aattcaaagg caatgcctca
    13621 gttctcccgg cggataacac gctggcaacg gttaccttta aaatcaccga ataaagccag
    13681 gataaaggat gatgaagata caatcgcgta aaatctggta ctaccgtata accctcatca
    13741 tcctgttgtt cgctatgctg ctcgcatggg cgctgcttcc cggcgtccat gagtttatca
    13801 atcgcagcgt tgcggcgttt gccgccgtgg accaacaggg tatagaacgc tttattcagt
    13861 cttacggcgc actggcggca gttgtctcgt tcttgttgat gattttacag gccattgctg
    13921 caccgctacc tgcgtttttg atcacctttg ccaatgcgtc gctgtttggc gcgttctggg
    13981 gcggcttgct gtcgtggacc agttcgatgg ccggcgcggc gctgtgcttt tttatcgcca
    14041 gagtgatggg ccgcgaagtg gtggaaaaat taaccggcaa aaccgtgctt gacagtatgg
    14101 acggcttttt cactcgctac ggcaaacaca ccatcctggt ctgtcggtta ttgccttttg
    14161 tccctttcga tccaatcagc tatgctgccg gtctgacttc aatacgtttt cgctcgtttt
    14221 ttatcgccac cgggcttggt caattaccgg cgactattgt ttattcctgg gcgggcagca
    14281 tgttaacagg cggtactttc tggtttgtca ccggactgtt tattctgttt gccctgaccg
    14341 tggtgatttt tatggcgaag aaaatatggc ttgagcgcca gaagaggaat gcctgatggg
    14401 tttaccgccg cttagcaaaa ttcctttcat tttacgtccg caggcgtggc tgcatcgtcg
    14461 ccattacggc gaggtgctaa gcccgattcg ctggtggggg cggatcccgt ttatctttta
    14521 tttggtgtca atgtttgttg ggtggctgga gcgcaaacgc tcaccgctcg atccggtagt
    14581 gcgatcgctt gtcagcgcgc gcattgcgca aatgtgcctg tgtgagtttt gcgtggatat
    14641 caccagtatg aaagtcgccg agcgtaccga cagcaccgat aaactgctgg cagtggctga
    14701 ctggcggcaa agcccgctct ttagcgatga agaacggctg gcgctggagt acgccgaagc
    14761 cgcaagcgtc acaccgccaa cagtcgatga tgccctgcgt acccgactgg ctgcgcattt
    14821 tgacgctcag gcgctcaccg aactgacggc attgatcggc ctgcaaaacc tgtcggctcg
    14881 ttttaattct gccatggaca ttcccgctca ggggctgtgc cgtattcctg aaaaacgttc
    14941 ttaaggagag atgatgcgcc attgtgggtg gttgctggga ttgttatcgc tgttttcttt
    15001 ggcaacacat gccagtgact ggcaggaaat taaaaatgag gccaaagggc aaaccgtctg
    15061 gtttaacgcc tggggcggcg ataccgcaat taaccgctat ctcgactggg ttagcggcga
    15121 gatgaaaacc cattacgcta taaacctgaa gattgttcgt ctcgcggatg ccgcagacgc
    15181 ggtgaagcgc attcagaccg aagctgctgc cggacgtaaa acgggcggct cggtggatct
    15241 gctctgggtg aacggcgaaa acttccgcac cttaaaagag gccaatttat tacaaacggg
    15301 ctgggcggag actctgccca actggcgcta tgtcgacaca cagctgccgg tgcgggaaga
    15361 tttttcagtg ccgacacaag gtgcggaatc gccctggggc ggcgcacaac tgacgtttat
    15421 cgcccgccgc gatgttacgc cacagccacc acaaacgccg caagccttac tggagtttgc
    15481 taaagtcaat cccggcacgg ttacctatcc gcgcccaccg gactttaccg gcacggcgtt
    15541 tcttgaacag ttgctgatta tgctgacgcc cgatcccgca gcattaaaag aagcgccgga
    15601 cgatgcgact ttcgcccgtg tcactgctcc cttgtggcaa tatcttgatg cgctacatcc
    15661 gtatttgtgg cgcgaaggaa aggatttccc gccttcgccc gcgcggatgg atgctctgct
    15721 gaaagccggc acattgcgcc tgtcgctgac ctttaacccc gcgcatgcgc agcaaaaaat
    15781 cgccagcggc gatttgccgg caagcagtta cagttttggc tttcgcgagg ggatgattgg
    15841 caacgtgcat ttcgtcacca ttccagccaa cgcgaatgcc agtgctgcgg cgaaggtagt
    15901 tgccaatttc ctgctctcac ccgatgcgca actgcgtaaa gcagatcccg ttgtctgggg
    15961 cgatccttct gttctcgatc cgcaaaaact gcctgacggg cagcgcgaaa tattgcaatc
    16021 aagaatgccg caggatctgc cgccggtact ggctgaaccg cacgcaggat gggtgaatgc
    16081 actggaacaa gaatggttac gccgttacgg tacgcattaa tctttttgct gtgggcgatg
    16141 gtggcggtga tttatgcacc gctgatcccg gcagctctca cgctgatatc gcctgcgttg
    16201 tcgttgacac actggcaggc gttatttgcc gatccacagt taccgcatgc attactggca
    16261 acgctggtgt cgacaaccat cgcggcggtc ggggcattgt tgattgccct gctggtgatt
    16321 gtggcgctat ggcctgggcc gaaatggcag cgtatgtgcg cccgtctgcc gtggctgctc
    16381 gccattcccc atgtggcttt tgccaccagc gccctgctgc tctttgctga cggagggctg
    16441 ctatatgact atttcccgta tttcactccg ccaatggacc gatttggcat cggtctgggc
    16501 ctcacccttg cagtgaaaga aagcgcattt ctgctgtgga tcttagcggc agtattgagc
    16561 gaaaaacggc tgttgcagca ggtcattgtg ctggattcgc tgggctacag ccgctggcaa
    16621 tgcctgaact ggctgctgtt gccctccgtc gcgccttcgc tggcaatggc gatgctggcg
    16681 attgttgcct ggtcgctttc ggtcgtggat gtggcgatta ttcttgggcc aggtaatcca
    16741 ccgacgctgg cagtaattag ctggcagtgg ttaactcagg gcgacgcgga tcaacaaacg
    16801 aaaggcgcac tcgccagtct gctgctgatg ctgttactcg ccgcctacgt tttgctgagc
    16861 tatctgctat ggcgcagctg gcggcgcact attccccgcg tagatggcat tcgcaagcct
    16921 gccacacctt tattaccagg cactacgctg gcgagttttt tacccttaac cggtgtgctg
    16981 tgtgtggttc tgctggcgat cctcgcggat cagtcgacga tcaatagtga agcactcatc
    17041 aacagtctga caatggggct ggtagcaaca ttcatcgctt tgctcctgtt actgctgtgg
    17101 ctggaatggg ggccacagcg tcgccagttg tggctatggt tgcccattct attgcccgct
    17161 ctgccactgg tggcgggcca gtacacgctg gcgctatggc tgaacctgga tggaagctgg
    17221 acggcggtgg tctgggggca tctgctgtgg gtaatgccgt ggatgctgtt tatcctgcaa
    17281 cccgcctggc agcgcattga ttcacggtta attttgattg cgcaaacact gggctggtcg
    17341 cgggccaaaa tcttctttta cgtgaaatgc ccactcatgt tgcgccctgt gctgattgcc
    17401 ttcgcggtgg gattttcagt cagtattgcg cagtatatgc cgacgctgtg gctgggcgcg
    17461 gggcgttttc cgacgctcac cactgaggcg gtggcattaa gcagcggcgg cagcaacggt
    17521 attctcgccg cccaggcttt atggcaactg ttgttaccgc ttattatttt tgccctgacc
    17581 gcgttagtcg caaaatgggt aggttatgtc agacaaggac tccgctaatg ctctgcgtga
    17641 aaaatgtttc gctacgttta ccagaaagcc gcttgctgac aaacgttaac tttacggttg
    17701 ataaaggtga catcgtcacg ttaatggggc cgtctggctg tggaaaatcc actctgtttt
    17761 catggatgat tggtgcactg gctggacagt tttcttgtac aggtgagcta tggctcaatg
    17821 agcaacggat tgacatgcta cccaccgcac agcgtcagat tggcattctt ttccaggatg
    17881 cactgttatt tgaccagttc agcgtcgggc aaaatttact gctggcgcta ccgtcgacgc
    17941 tcaaggggac tgccaggcgc aatgccgtga aagacgcgct tgatcgagcg ggactggctg
    18001 aaacttatca ccaggaccct gcgacattat caggcggtca gcgagcgcgg gtggccctgc
    18061 tgcgcgccct tctcgctcag ccaaaagcgt tactcctgga tgagccattc agccgtcttg
    18121 atgtggctct gcgcgataat tttcgccagt gggtgttcag cgaagttcgc gaactggcga
    18181 tccccgtcgt tcaggtaacg cacgatctcc aggatgttcc tgctgatagt tctgttctgg
    18241 atatggcgca gtggtcagaa aattacaaca aactgcgata acgcaaagtt tttctcaatg
    18301 cgtcagttca gaatggcgca ctcaaaacta caatgtcggg attttcgatg aaacgtgttt
    18361 ctcaaatgac cgcgctggca atggctttag ggctgacttg cgcttcttcg tgggccgctg
    18421 aactggcgaa gcctcttaca cttgaccagc ttcaacaaca aaatggcaaa gcgatagata
    18481 ctcgccccag cgcgttttat aacggctggc cacaaacctt aaatggccct tctggtcatg
    18541 aacctgccgc cttaaacctc tctgccagct ggctcgacaa aatgagcacc gaacagctct
    18601 atgagtggat caagcaacat aatctgaaaa ccgatgctcc ggtggcgctg tacggcaatg
    18661 acaaagatgt cgacgccgtc aaaacgcgtc tgcaaaaagc aggttttacg catatctcca
    18721 ttctgagtga cgcgctaagc gaaccttccc gtctacaaaa actgccgcac tttgagcagc
    18781 tggtttatcc gcaatggctg cacgacctgc aacaaggtaa agaggttacg gcgaaacctg
    18841 ccggtgactg gaaagtcatt gaagcggcct ggggcgctcc taagctttac cttatcagcc
    18901 atattcctgg cgctgactac atcgatacca acgaagtgga aagcgaaccg ctgtggaaca
    18961 aagtttctga tgaacaacta aaagcgatgc tggcaaaaca cggcattcgc catgacacca
    19021 cggtcattct gtacgggcgt gacgtatacg ctggagcgcg tgtggcgcaa attatgctct
    19081 acgcaggcgt gaaagatgtg cgtctgctgg atggcggctg gcaaacctgg tccgacgcgg
    19141 gactgcctgt tgagcgagga acgccaccga aagtgaaagc ggaaccggat ttcggcgtga
    19201 agatcccggc acaaccgcag ttgatgctcg atatggaaca ggcccgtgga ctgctgcatc
    19261 gccaggatgc atcgctggtg agcattcgtt cgtggccaga atttatcggt acgaccagcg
    19321 gttacagcta tattaaacca aaaggtgaaa tagccggagc acgttgggga cacgctggta
    19381 gcgactcgac gcatatggaa gattttcata acccggatgg caccatgcgc agcgccgatg
    19441 atattaccgc tatgtggaaa gcgtggaata tcaaaccaga gcagcaagtt tcattctact
    19501 gcggcaccgg ctggcgcgcg tccgaaacct ttatgtacgc acgcgccatg ggttggaaga
    19561 atgtttccgt gtatgacggc ggctggtacg aatggagcag cgatccaaaa aatccggtag
    19621 caaccggtga acgcggcccg gacagtagca aataacattg caattactga cgctggagcg
    19681 acttcagcgt cagataccca ctccacaccc gcgtaaaggt cgtcatccag cacaacgcac
    19741 cgaagatcca cgcaaaccac gcaaaccatg cagggaataa acatcccagc acaaacagta
    19801 agatcgtttc tgtgccttcg gttaatcccc ccagatagta aaacgactta tgtgcataac
    19861 cggggttatc aatctgatgt tttgctgcca gtgcagcaaa ggcgagaaaa ctgctgcccg
    19921 tgccgataaa cgcaaacaac aaccagccgc ctgccagcgc attttgctcc ggtgcagcaa
    19981 gaataaagcc aaacggcacc agcgcgtaaa agagaaaatc gagagaaata tcaagaaagc
    20041 cgcccgcgtc ggtaagctct ctgcgccgcg ccagcgcacc gtccagacca tcaagcagcc
    20101 tgttcaacaa aatgacgact agcgccgcca gataccagcc cagcgccaga aacggcagag
    20161 ccaacacgcc gatggcaaat cccactaatg ttaaaccatc tggcgtaatg cccggtttat
    20221 caagaacccg cacgcactga tgcaacaacg gtttaatccg gggatgaaga tggcggtcta
    20281 gcacaggcac tccttaaata taaagccttt ctgattgagc aacagtgcgg atattatggc
    20341 atttttcgct tatctgcccg tgtgtaattt atgaaaatga ttgaagttgt tgccgccatc
    20401 attgaacgtg atggcaaaat tttactcgcg caacgccccg cccagagcga tcaggcggga
    20461 ttatgggagt ttgccggtgg taaagtcgag ctggatgaaa gccagcagca ggcactggtg
    20521 cgtgagttaa acgaagaatt gggcatcgaa gcaactgtgg gtgagtatgt tgccagccat
    20581 cagcgagaag tttcgggccg gattatccat cttcatgcct ggcacgtacc cgacttccac
    20641 gggacgttac aggcacatga acatcaggcg ctggtctggt gctcacctga agaggcgctg
    20701 caatatccgc tggcccctgc tgacattcca cgattagagg cgtttatggc tttacgcgcc
    20761 gccagagcag cggattagtg ctaagggttt tgtcatcccg ctggcattgc agcagtattc
    20821 cttcggcttt aattaccgcc ccttcagaat aattttgatc ctgataaacg cagcactgag
    20881 tacagggctg cgctgactgc ccgcctgaac tgaacacttc tggcggtacg tttacctcca
    20941 cgtccggacg ataatgcggg ttagccagtg caattaatgg aaatgctaat actacggcga
    21001 acaatgctcg actcacaggg aactccttaa cgttattgtc tctgcaactg ataacggtag
    21061 ccgggtggca aaactttagc gtctgagtta tcgcatttgg ttatgagatt actctcgtta
    21121 ttaatttgct ttcctgggtc atttttttct tgcttaccgt cacattcttg atggtatagt
    21181 cgaaaactgc aaaagcacat gacataaaca acataagcac aatcgtatta atatataagg
    21241 gttttatatc tatggatcag acatattctc tggagtcatt cctcaaccat gtccaaaagc
    21301 gcgacccgaa tcaaaccgag ttcgcgcaag ccgttcgtga agtaatgacc acactctggc
    21361 cttttcttga acaaaatcca aaatatcgcc agatgtcatt actggagcgt ctggttgaac
    21421 cggagcgcgt gatccagttt cgcgtggtat gggttgatga tcgcaaccag atacaggtca
    21481 accgtgcatg gcgtgtgcag ttcagctctg ccatcggccc gtacaaaggc ggtatgcgct
    21541 tccatccgtc agttaacctt tccattctca aattcctcgg ctttgaacaa accttcaaaa
    21601 atgccctgac taccctgccg atgggcggtg gtaaaggcgg cagcgatttc gatccgaaag
    21661 gaaaaagcga aggtgaagtg atgcgttttt gccaggcgct gatgaccgag ctttatcgtc
    21721 atctgggcgc ggataccgac gttccggcag gtgatatcgg cgttggtggt cgtgaagtcg
    21781 gctttatggc ggggatgatg aaaaagctct ccaacaatac cgcctgcgtc ttcaccggta
    21841 agggcctttc atttggcggt agtcttattc gcccggaagc taccggctac ggtctggttt
    21901 atttcacaga agcaatgtta aaacgccacg gtatgggttt tgaagggatg cgcgtttccg
    21961 tttctggctc cggcaacgtc gcccagtacg ctatcgaaaa agcgatggaa tttggtgctc
    22021 gtgtgatcac tgcgtcagac tccagcggca ctgtagttga tgaaagcgga ttcacgaaag
    22081 agaaactggc acgtcttatc gaaatcaaag ccagccgcga tggtcgagtg gcagattacg
    22141 ccaaagaatt tggtctggtc tatctcgaag gccaacagcc gtggtctgta ccggttgata
    22201 tcgccctgcc ttgcgccacc cagaacgaac tggatgttga cgccgcgcat cagcttatcg
    22261 ccaatggcgt taaagccgtc gctgaagggg caaatatgcc gaccaccatc gaagcgactg
    22321 aactgttcca gcaggcaggc gtactgtttg caccgggtaa agcggctaat gctggcggtg
    22381 tagcaacgtc gggcctggaa atggcacaaa acgctgcgcg cctgggctgg aaagccgaga
    22441 aggttgacgc acgtttgcat cacatcatgc tggatatcca ccatgcctgt gttgagcatg
    22501 gtggtgaagg tgagcaaacc aactacgtgc agggcgcgaa cattgccggt tttgtgaagg
    22561 ttgccgatgc gatgctggcg cagggtgtga tttaagttgt aaatgcctga tggcgctacg
    22621 cttatcaggc ctacaaatgg gcacaattca ttgcagttac gctctaatgt aggccgggca
    22681 agcgcagcgc ccccggcaaa atttcaggcg tttatgagta tttaacggat gatgctcccc
    22741 agggaacatt tcttatgggc caacggcatt tcttactgta gtgctcccaa aactgcttgt
    22801 cgtaacgata acacgcttca agttcagcat ccgttaactt tctgcgatag cagcagatat
    22861 gccagtaaag aaatcccatt agactatttt tttgataatc ttcttcgctt tcgaacaact
    22921 cgtgcgcctt tcgagaagca agcattatat aatgccaggc cagttcttct tcaattgtcc
    22981 cgttttgaaa agctgtgctt gatatcgaga tcatccatga taattccgcc gcccatatta
    23041 gcttcgccga ggatttaccg gagcgattat tagcgcaatc agagatatag tctgagggaa
    23101 aaacagcaaa tttattcaac aaggcgataa cctgctctgg ggcttcctcc atgtttgctt
    23161 taaaggtatt ggctccatgg tcgccagaaa gaaaatgctc cattaaggca caataacttt
    23221 cgctatcttc gataccccat tgatcctcta aagactcgcg tcttttactt atgatatcga
    23281 tcgagtcaaa aggaagcaca tgatattgga aggtatcttt gccaggttca ggctttcgcg
    23341 gccagaactc cagcgtttca gaccattgct tatgatagaa tcgataaggt gcgatcaatt
    23401 gtagcgcctg taacttctcg atactgagcg gctcaatacc tttagcctga taataatgca
    23461 gttgttcttt ttttgcttta aaaccggccc gaacaataag ccccatcata attaatagat
    23521 aaagaaaaga gcatcccgcg gtaatcaggc ctctttcatt caaaccgttg gatgttatcg
    23581 ctgcgaacac aaacattaca gcgacaacac atgttaaata acccccccac ttacaaagca
    23641 gcatggcctt attttcttta atcatccgtt caaaattact attaaatatt tcccagccat
    23701 taaaagaata cttctcgctc ccaggatggt tttgtaataa aacttttttc atcacattcc
    23761 ctgttattac atactttaga ggcgataatt ttatccatgc aaaaaaaata tccaacgaat
    23821 tttcttgatc cgctggatat ttcagaatat gactcgaata gcacgaaaga ttcgctcgct
    23881 tacgctatcg ccccgcttcc gacttcatct gctggcggac tttttttcgc actacgttta
    23941 cgcggtgcag cctttttctt atcagcactg acaccactgc ccggagccac aatgccgcga
    24001 aactgccgca ccggcgtacg tttggcttga tcaataagct gatatagcgt ccccaccagc
    24061 ggctgcataa agtcctgata gcgacactgc ttttcgctga tttgcgtcag caccgattcc
    24121 cagtgcgcgg tcatgtccgg tcgcgtcgcc atttctggca gcgaatggaa taacgctttt
    24181 ccggcgtcgg tggagtggat atagcgccct tttttggtca gaaaaccacg cttgaacagc
    24241 agttcaataa tcccggcacg ggttgcttct gtccccagac catcggtcgc acgaaggatc
    24301 tttttcagat ctttgtcctg cacaaagcgc gcgatcccgg tcatcgccga aagcagtgtt
    24361 gcatcggtaa aatggcgcgg cggctgggtt tgccgctcta ccacttcacc tttttcacac
    24421 agcaactcat cgcctttcgc caccacaggc aatggcgtgc cgtcgttttc ttcatcgcgc
    24481 tctttgctgc ctaacagcgt gcgccagcct gcttcagcaa gaaaacgggc tttagcgaca
    24541 aatttgcctt tggcaatgtc cagttcgata acacacttgc ggaacaccgt atccgggcag
    24601 aactgcatca ggtattgacg ggcaatcagg ttatagacct tcgcttcgtt ctccgtcagg
    24661 ttgatcgcag aactccgtgc ggtcgggatg atggcgtggt gtgcatcgac ctttttgtca
    24721 tcccaacagc ggttgcgtat atctggatct accactggct gcggcaacag atctggtgca
    24781 tgaacgctga tggcattcat caccgcgtgg cgtccggcaa aatgttcttc tggcaaatag
    24841 cgacaatcgg aacgcggata agtgattagc ttgtgcgttt cgtacagctt ctggcagata
    24901 tcaagcacgt tctgcgcgct cagaccaaag cgttttgccg cttcaatctg caacgccgaa
    24961 agcgaaaaag gcagcggcgc ggattctgat tcccgtttat cgttatagct ggtgacaata
    25021 gctggttgac cactaatgcg gttaaccaca tgctccgcca gtggacgatg taacaagcgc
    25081 ccttcttcat cctggtacgg ttcacacgct tcgctcggtt gccagatagc ggtaaaccgc
    25141 tcatcggcag gtgtcacgat atgtgctttg acttcaaaga agtctttcgc cacgaagttt
    25201 tcaatctctt catcgcggcg caccaccagc ccgagcacgg gtgtctgcac gcgtcccacg
    25261 gaaagtacgc cctgataacc ggcattgcga ccgagaatgg tatacgcacg ggtcatattg
    25321 atgccgtaca gccagtcggc acgcgctcgc gccagcgcag aaacgcacaa tggcacaaac
    25381 tcactgttgg accgaagacg gtcgatcgcc cgctcaaccg cctgcgggtt caggtcgttt
    25441 atcaagcaac gctgcacctg ctggcgcttt tccggtgcca gttgcagata gtccagcact
    25501 tcatccacca gcagttgccc ttcacgatcc gggtccccgg cgtgaacgat ttcgctggct
    25561 tcatgcagga agcgtttgat gacgttaagt tgtttggtca cggagggtcg gggctgtaat
    25621 tgccactttt ccgggacaat cggcaaatcc gcaagattcc agcgcgcata gcggctgtcg
    25681 taggcgtctg gctgcgcctg ctcaagcagg tgaccgatac accaggtcac cacctgacca
    25741 ttaccgcact cgataaagcc atcgcctttc cggtgcggtt tgggcaggac atcagcaatg
    25801 gcgcgcgcca gactcggttt ttcggcaata aacaaccgca ttgaattaac gaatctcaac
    25861 catggcacga ccgccgcgcg ctggcaccag ttcgccaatt gccgtcagtt caatgccaaa
    25921 ctcggcggct gtagctttga cctcattttc tgcttccggc atgaccgcca gcagcaaacc
    25981 gccagaagtt tgcggatcgc acagcagatc gcgcacttca cgcggcattt cccccatcag
    26041 atgaccgtag ctggcaaagt tacgttcagt gccgccaggt actgcgccca acttaatgta
    26101 ctcttcaaca ccggggagtt tcgggatcgc ttcatagtcg acgcgtgcct gcacaccagc
    26161 cccctgacac atttcgctca agtggcccag cagaccaaag cccgtaacgt cggtcatcgc
    26221 ttttacgcct tcgatgttgg caaaggacgc gcctgcgatg ttcatccggc acatcacttc
    26281 cgtcgccagt ccctgatgtt ctggtttcaa cagtgatttt ttctcagccg tggtaagaac
    26341 gccgatcccc agcggtttcg tcaggaacag tttgcatccg gcttgtgcgg tactgttttt
    26401 cttcacccgc tcggtcggta cgatccccgt taccgccaga ccaaaaatcg gctccggcgc
    26461 atcgatggag tgaccgccag ccagcgcaat acccgcctga cggcatgcat agcgtccacc
    26521 ttcggttact tcgcgggcaa tttccgggga aagtttgtta atcggccagc cgaggatcgc
    26581 aatcgccata atcggtttgc cccccatcgc gaagatatcg ctgatggcgt tagtcgccgc
    26641 aatgcggcca aaatcgaaag gattatcaac gatcggcata aagaagtcgg tggtactgat
    26701 aacgctggtg ccattgccca gatcgtacac cgccgcatcg tcgcgggttt cattacccac
    26761 aagcaaattc ggatcaacaa acttcgcctg ctcactgtgc aggatggttt ccaacacttt
    26821 tggggaaatt ttacagccgc aaccagctcc gtggctgtat tgggtcaaac gaatcgagtt
    26881 ctcgctcatg gacatctcct gtcaatgcaa tccgggtatg gtaaccctca ttccgtgaag
    26941 tgataagtga gagtgtctga attcctgcgc ctttgctcac aatccagaca gtttcgcgac
    27001 aattatcaga aataagttac aaacggcgtc gggtccggga cgttaatcga cgtagatgct
    27061 ttcaactgcg gcgtgccgag gtagagaaaa ccgacaattt tatcctgctc acggcaaccg
    27121 aatgcttcac gcactaccgg actttcagtt aatgcgccac tgcgccagat gccgccaaat
    27181 ccctgggcaa cggctgccat ttgcatcgcc atgaccgcgc atccggcaga catttcctgt
    27241 tcccatcgcg ggactttatg attttcttcg catttcgcca ccaccgtgat gatgagcggt
    27301 gcgcggaacg gcgcattacg ggctttgtcg atagctttgt catcactacc ggcagcaatc
    27361 gccccctgtt ccagtacggc gctgaaacgc tcgcgccctt ccccttcaat cacaaaaaaa
    27421 tgccacggtt gcatggactt atggtccggc gcgcgcatac ccgcacgcag gatgttttgc
    27481 agttgttcac ccgttggtgc gggttcagcc aggcgggagg cgctacggcg attgatcaat
    27541 agttcgagtg catccatttg attaactcct gtcgtgatat ttattcacaa aattaacacg
    27601 agagtggatt ttgttacagc acagtccgca attcctgctg acaagtgccg gttgggtcat
    27661 tacgataacc acatctattg cgcctgtgac aggtgtgacc ttaagttggg agaatacatg
    27721 cgaacccttt ggcgatttat tgccggattt tttaaatgga cgtggcgtct gctgaatttc
    27781 gtccgtgaaa tggtacttaa cctgttcttt attttcctcg tactggttgg tgtggggatc
    27841 tggatgcagg tcagtggtgg tgattcgaaa gaaacggcca gtcgtggcgc actgctgctg
    27901 gacatttctg gtgtgatcgt cgataaaccc gacagttctc agcggtttag taaattaagc
    27961 cgccagctgc ttggtgccag ttccgatcgt ctgcaggaaa actcactgtt tgatatcgtc
    28021 aacaccattc gccaggcgaa ggacgaccgc aatatcaccg gtatcgtgat ggatctgaaa
    28081 aacttcgcag gcggcgacca gccgtctatg cagtacatcg gcaaagccct gaaagagttt
    28141 cgtgacagcg ggaaaccggt ttatgccgtt ggcgagaact acagccaggg gcaatattat
    28201 ctcgccagtt tcgccaataa aatttggctg tcaccgcaag gcgtggtgga tttgcacggt
    28261 tttgctacca acggtctgta ctacaaatcg ttgctggata agctgaaagt ttccacccat
    28321 gtgttccgcg tgggtacgta taaatctgcc gttgaaccat ttattcgtga tgacatgtca
    28381 ccggcagccc gcgaagctga cagccgctgg attggtgagc tgtggcaaaa ctatctgaat
    28441 actgttgccg ctaaccggca gatccctgct cagcaggtat tccctggcgc gcaagggttg
    28501 cttgagggtt taaccaaaac cggtggcgat accgcgaaat atgcactgga aaacaagctg
    28561 gtcgatgcac tggcatcgag tgccgaaatc gaaaaagctc tgaccaaaga gttcggctgg
    28621 agtaagactg ataaaaatta tcgcgccatc agttattacg attacgcatt gaaaacgccg
    28681 gcagataccg gtgacagcat cggtgtcgtc ttcgctaatg gcgcaattat ggatggcgag
    28741 gaaactcagg ggaatgttgg cggtgatacc actgcggcac aaatccgcga cgctcgcctt
    28801 gacccgaaag tgaaagcgat tgtcctgcgt gttaatagcc caggcggcag cgttaccgcg
    28861 tctgaagtga ttcgcgctga actggcagca gcccgggcag cgggtaagcc tgtggttgta
    28921 tcgatgggcg gcatggcggc atctggtggt tactggattt ccacgccagc taattacatt
    28981 gtggctaacc ccagcaccct gaccggttct atcggtatct tcggcgtgat caccaccgta
    29041 gaaaatagtc tggattcgat tggtgttcat actgatggtg tctcaacttc accgctggcg
    29101 gatgtttcta tcaccagggc actgccgccg gaagcgcagc agatgatgca attaagcatt
    29161 gagaatggct ataaacgctt tatcacgctg gttgctgatg cgcgtcattc gacgccggag
    29221 caaattgata aaatcgccca gggccacgtc tggaccggtc aggatgcaaa agctaacggg
    29281 ctggtcgata gtctcgggga tttcgatgat gcggttgcca aagcagcaga gctcgcaaaa
    29341 gtgaaacagt ggcatctgga atactacgtt gatgaaccga ccttcttcga caaagtgatg
    29401 gacaacatgt ctggttctgt ccgggcaatg ttgccagatg cgttccaggc catgttacct
    29461 gcaccgcttg cctcggtagc ctctaccgtt aaaagtgaaa gcgacaagct ggcagcgttt
    29521 aacgacccac aaaaccgtta tgcgttttgc ctgacctgcg ccaacgtgcg ttaagtcttg
    29581 tactgagtgg ccgacagatc gtcggccaca tttttttacc tcgacgaatc ctcttcccgc
    29641 tgtctcgccc catatttcct tatccgcata gtatcaggtg cgctccccct gcctcacgta
    29701 tatacttttg ctctttcgat atcattcata tcaatatcat gcaaaagaaa tcaatttacg
    29761 ttgcctacac gggcgggacc atcgggatgc agcgttccga gcagggttat attccggtgt
    29821 caggtcatct acaacgccaa ctggcgctga tgccggaatt ccatcgcccg gagatgccag
    29881 atttcaccat tcatgaatat acgccgttga tggattcttc tgatatgacg ccggaagact
    29941 ggcagcatat tgctgaagat attaaagcgc actatgacga ctatgatggt tttgtcattc
    30001 tacacggtac cgacacgatg gcgtataccg cctctgcgct gtcgttcatg ctcgagaatc
    30061 tcggtaaacc ggtcattgtg acagggtcac aaatcccgct ggctgagtta cgctctgacg
    30121 gacaaattaa tctgctgaat gcgttgtacg ttgcggcgaa ttatccgatc aacgaagtaa
    30181 cgctcttttt caataaccga ttgtatcgcg gcaaccgcac taccaaagcc catgccgatg
    30241 gttttgatgc gtttgcctct ccaaaccttc ctccgttact ggaagctggt atccatattc
    30301 gtcgtttgaa tacgccaccc gccccgcacg gtgaagggga attaatcgtt catccaatca
    30361 cgccacaacc aattggcgta gtgacgattt atccagggat ttctgctgac gtcgtgcgca
    30421 attttctgcg ccaaccggtg aaagcattga ttctgcgctc ctatggcgtg ggtaatgcgc
    30481 cacaaaacaa agccttcctg caggaattac aagaagccag cgatcgcggt attgtggtgg
    30541 tcaacctgac acaatgtatg tccggtaaag tgaatatggg tggttatgcc accggtaacg
    30601 ccctcgccca tgccggcgtt attggcggtg cagatatgac tgtagaagcc acgctaacca
    30661 aactgcatta cctgctgagc caggaactgg atactgaaac cattcgcaag gccatgagcc
    30721 aaaacctgcg cggcgaactg acgccggatg attaaggaga ctgcaatgcc ccatcgcgcc
    30781 ctgttactgg tcgatttaca aaatgatttc tgtgctggtg gcgcgctcgc cgtaccggaa
    30841 ggtgacagta cggtggaggt tgctaaccgc cttattgact ggtgccagtc gcgcggagaa
    30901 gcggttatcg ccagccagga ctggcatccg gcgaatcacg gcagctttgc cagccagcac
    30961 ggcgtagagc cttatacgcc aggccaactc gacggcctgc ggcaaacttt ctggcctgaa
    31021 cactgtgtgc agaacagtga aggcgcacaa ttacatccgt tactgaatca aaaagagatc
    31081 gcagcggtgt tccataaagg cgaaaatcct ttagttgaca gttacagtgc cttttttgat
    31141 aacggccgtc ggcagaaaac ctctctcgat gactggttac gcgatcatga aatcgatgaa
    31201 ttgatcgtta tgggcctggc tactgactat tgcgtgaagt ttaccgtgct ggacgcgtta
    31261 cagttaggtt ataaggtgaa cgtgattacc gatggttgtc gtggcgtgaa tatccagccc
    31321 caggacagtg cgcacgcgtt tatggagatg tcagcagctg gggcaacgct atatacgctg
    31381 gcagactggg aagagacgca ggggtaattt tacgctggcc tacaattctg tactggcatt
    31441 gtaggccaaa taaaacacgt cagtggtaca tctggcaatt gatgccatca acgaaagatt
    31501 taattcacct cagaaatctc ttccaacgac accttccgcg tttcgatgcc aaaaatggag
    31561 agaaccagcg cacagagcaa taacatgaca ccaagtacca taaacaccgt gatcgaccca
    31621 taatgtgtta ataatgccgc aacgccatag ggcgtgaaga ctgcgacgat ccgtccgaca
    31681 gcattaacga aacccgaacc gcgcaggcgt aaatgcgttg gccaaagctc cgggatataa
    31741 accgccgacg cgaagcaaac atacatgtat aaaaagaaga tcatcaccag accatagatt
    31801 aaaatcgccc cctctgtagt ctgaattgaa tagatatagc ctaacacggc aataataata
    31861 agtaaggcgg agccaaataa tcgacgagga aaatgatcaa taattaatgc cgcaataaat
    31921 attcctaccg gagcaccaat cataataaca gcggtcatta atattgattt atcgacatca
    31981 atgccggagt taacaaatat ggtcggtatc catacggtaa tggtataaag cgaaatgttc
    32041 attgcaatta aaacagttat cgcaactaat gtacgtcgta acatttcccc tttaaacagg
    32101 agccagaagg tacccttgat taccttaacc ttgctgttgc tctggttcaa agtaagctgg
    32161 ggtaaacgaa tactcttctc cctttcaatt tgctgctcta cttcacgaag ttggctttct
    32221 gcaccggcga tttgcccttt ccctgccagc catcgtggcg actcgataaa gtatttacct
    32281 gagagaaacc aggctaacag tatgccaata ccacccagca gaaacattat tcgccaacta
    32341 aaaaaagcga taaccaccac gcctatcgcc gcagacagca tgggcgacca gttaccaaca
    32401 aatgagagcc gcgcggacca ttttccacgc accgtcgcgg ggataaactc cgtaaatgag
    32461 gcatagccaa ccataatcag cgcccccatt cctgttccca tcaggaagcg aaagaaaatg
    32521 agccagtaca tatcagggac aaaagcggcc cctgttgcag caataccgac gatgagaaga
    32581 tttatgcgaa acgccctgcg ccgcccaaag tagtcaccaa taaacccacc agtaagtgag
    32641 ccgatgaaat aaccaaacat taatgccgag gtaaaggcgg cattgaggaa attatttgac
    32701 catccattgc ttaccagctt tgctaggacg acattacctg agtaactcaa aaaccccgtt
    32761 aataacagac taaagcttat tataccaaat atacgataat gaaaccgggc caaaggcaag
    32821 ctgtccagtc ttgcgccaat ttgatcatat tgttccattg ggtgcctcgg atttgctaaa
    32881 aaaggtagca attgcggcaa attcacttgc cgcatcttta tcatcatttt atttccaggc
    32941 gcaggaacaa ataatattga caacgtgtgc ttaaattacc acaccagctg gatattattg
    33001 tctttgcaaa atgctatcca ctctgcaccc ggtgatttat cagttattat gtaattaata
    33061 tgactaaagt cagctaactg gacaaaagct ttgcgatcaa acttagagtg atcaaccatt
    33121 aacgcaactt ctgtcgcctg acggatcatt gtctttttga tttcagcttc cgcttcatta
    33181 gagtccagcg cgccgctgtt aatatcaaga cctttacaac tcattaccat gatatcgaca
    33241 tgatagcggc tgatgatctc tttagtaatt cttccttgca gggaaagtgt atttttgttt
    33301 agttccccac ccgttgaaac gactttaatt tctgactgag ccagaacatg aatcgcttcc
    33361 gccgagttgg ttagcaacgt caggtcacta cggtcctgta acaatttgag caattccatt
    33421 acggtactac tggaatcggc tgccatggtg gttttattgt cgataaaggg tagtgccttg
    33481 cgtgcaataa gctgcttctc ttcataaaac gatgaagcgc gcttataaaa atggatattc
    33541 tccgtcaaca tcgctgtatt taaaacagca ccaccatagg ttctggtcaa aaaaccttca
    33601 tcttccagct tctcaagatc gcggcgaatg gtttcttcgg ttacctgaaa aatcccactc
    33661 aaatttgaga ctgtcacctt tttatcgttg gcaaccattt gcttaattgc ctgaatcctg
    33721 tcttttgccg ccacgattac acccctgtat ctttttacat cacattagcg cgattatcgc
    33781 ataaccgatg tttactttca aaataacctg tttgaatcac agattttcat cacagttttc
    33841 acagaaacag aggtgaatcg tgttgagtat ttaacgctct agggcctctg ccatttccct
    33901 catcaatgtt gcgtctgcat ccgataagtt gatattcagt gccgcgacat tttcgcgtac
    33961 ctgttccggt gcagtagccc cactaagaat ggagattaaa tcactctgtt ttaatatcca
    34021 cgccagtgcc agagtgggaa ttgtgcactg ataacgagca caaagtggct gccactgttc
    34081 aagcatatca atcactttca gcatgttttc acgctggaac cagactttat ttgcccgagc
    34141 gccgcccgga acgtaatcac gagtgatggt gccggtcaac aatccctgct ctagcgggga
    34201 ataaacctga accacaatgc cattatcacg acatagcggc agcagttcgt tttccattgc
    34261 ccggtcgagg atactgtatt tcgcctgaat aatatccagt tcaccatatt gcagatactc
    34321 gcggatatgg tcagcatcga cgttagcagc gcctatagcg cgaattttcc cttcagcctt
    34381 taactcatta agcactgcga cagtttcagc gatcggcgta aaaaatggcg gcaccgactg
    34441 ccagtgcgtc atgtagatat cgatgtaatc aatacccaga cgttgcaagc tcgcttctac
    34501 ctcttcgcgg atagattccg gggaaaggtt tttatacaac tgccgatcgc caactttgtt
    34561 gaataaactt ccttttcgtt cccagacaat gccgcatttg gtttctacta caacctgttc
    34621 acggggcagt ttttttaacg cctgaccgac gataacttca ctattgccaa agttatatcc
    34681 tggcgcagta tcaatcagat taatgccaca acgatgggct tcaagaatcg tatcaataca
    34741 tatttgccga tcgagatcgc cattccatgc aggaccgccg ccaatggccc atgtccccaa
    34801 ccccattcgc gaaagcgtaa tatccgttgt gcctaaaggt atctttttca tttgccttca
    34861 ttatccttcg tattcttcca gcaattgttc gaccagcttt ctgtttttta cgccggtggt
    34921 ggcaccgacg cttagaaccg agatagccgc cgttgcattg gcaaagcgtg cgcattcacg
    34981 cagatttttg ccttctaaca gtgccgcaat aaaacctgaa gcaaagttat cgcccgcgcc
    35041 aatggtgtcg atggcggtta ttcctgcgac cgccggcacc ttcatcgtca tgtcaccacg
    35101 cttgataaag cagccgtctt tacccgtttt aatcaccacc gtttttacgc cgcacgcaag
    35161 aaagcagtca gcaatttcat ccagtgtctc tttcccggtg agtaattttg cctcagcata
    35221 attaggaaac agataatcga cataactcaa tgcttcgcaa atatcatcca gcgtttcatt
    35281 caaccgcggt ttgatcatat cggcacaaat gatcatctgc cgggctttcg cttgcgtaaa
    35341 aatttctgtt agcgctttac catccaatag aggactgttg aaaatactgg caagtgataa
    35401 taatttcgcc tgaggaaacc gctcaaaatc aacatcgtca atatttaatt tccacaaact
    35461 gccgttacgg ttcgtgacaa acgtccgctc gccatcctcc gtcaccagtc caacgttaat
    35521 agatgtatct atgctgacgt cctgtttcag gctttgaata tcaatattct ctttgcgaca
    35581 atggtcgaga ataaattgcc ctgaggcatc tttaccaatg cggctcatta atgcggtacg
    35641 atgccccaga cgagaaataa ttgttgcttc atttattgca tcgcccccgg tggtcattgc
    35701 gattctttca agaggataag aatctacatc aaagatattt ttactgaccg gttgtaatgg
    35761 aatatcaaca atagcggcac ctatacaaat aacgtcgaga ttatccatat cattattccg
    35821 ctttgccatc agaaccgaac agtttaattt tttccaatgc ccgttctttt acagctttac
    35881 gtacttcacg ttctaaatgc aggaaaggct gatcctggtt ttctttcact gctaccattg
    35941 cggcctgaca gagctccgta tgaatgttga ttttcgcgat acccaacgaa attgcagttt
    36001 taatgtcggc atcactgatc cccgatgcgc catgcaaaac cagcggaaca gaaacggcat
    36061 cgcgtacgcg tttgacgacc tcaaagttca attgcggctc tgacgtataa accccatgct
    36121 ggttgccgat ggcgacagcc aatgagtcac agcccgtacg ttcaacaaat tcagccgcct
    36181 ggtcaggatc ggtatagtga tagcccgcca gcgcttcctc ataaacggtt tcattaccga
    36241 catgccctaa ttccgcctct accggaatac ccagcggatg gaagaaatca acagcctctt
    36301 tggttaaacg aatattttct tcgaaatcaa atgcagaagc atcacgcatt aatgaattca
    36361 taccatgagt ccaggcgtta tgaataatct ccatactccg accatgatcc cagtgagtta
    36421 ttactggcac agttgctttt cgtgccattg ataccatcat gtgagagaaa tcttcaaatg
    36481 aggtgttgcc gacaaaacct gtaccaaaag aaataataac cggtgatttc gcttcttcgg
    36541 ctgcgtcgat aacgcccatc aacatttctg cattccatac gttaaaatgg gcgattgcat
    36601 aatgtttatt tgtggcatcg ttttcccaat atctgatatc tgcgagcatg ttctgttctc
    36661 ccgtattaat taatcgctaa ttttaataac gcctttaata atgtcgcgtt tgttgttaac
    36721 tgactcttca aatgcctgtt gtacatcccg ataatcgtaa atatgcgtca ccatcgattt
    36781 cacatcgaat cgccctgaag aaatagcttc aatcgtgacc ggataacgat tggcatagcg
    36841 gaataccgtc tggatagtga cttcgcgatt gattttgagg aaattgattg ccgaatcgcc
    36901 gggtacagta ccaacaatca taattttacc gccgcgcatt accagataag gtgcctgttt
    36961 aacggtgatc gcagaacccg ctgtttcgaa aacaatatct gcgcccatgt cttcggtaaa
    37021 ttgctgacag cgtgcaatag tgtcctcttt tgcgccgtta ataaccactg tcgcaccaag
    37081 ctgttccgcc attgccagac gtttttccag tacatcaacg acggcaattt ccgttgcgcc
    37141 caagcatttg cacgcttgca acgtcatcaa accaatacaa cctgctccca gaataattat
    37201 cttcttaccc ggtttaacat ctgccagcat cgcggcatgc atcccgactg cggcaggctc
    37261 caccagcgcc ccttccatcg tgtccatatt gtcgggcagt ttgtaagtaa agctctccgg
    37321 atgacacaga tagtgcgtta atgcgccgcg gtagttgggt tgtgtcgcca taaaatcaac
    37381 gtccgggcag atgttatatt tgccttccag acagtaacga cagtgaccgc aaggaacgcc
    37441 aggttcgata tttacccgat cccccggttt aaatttgcgc acgcggcttc ccacagccac
    37501 aaccgtcccg gcgcattcat gacccaggcc aatttcttga tttgggtctt taggcggaat
    37561 aaacgggcct gattcaaaac catgtacatc tgaaccacaa ataccgacat attctacttt
    37621 aatcaaaact tcatcttctt taggcactgg tatttctgct gaaataattt tcattgtgcc
    37681 cggcacctgc aatattgctt ttgaattttt cattttgaat ccacccgtat tatttattgg
    37741 ctactgcatc aatacttaaa ctttcaacgg agacaccttt ggtttcaatt ccaatggtgg
    37801 cgatagcaat tgcgacaaca attgaaacag cccccagaag aataaagact cccgttacgc
    37861 cataactact gagcagcact gcaacggcat aaggtgcggc aataccactg attcgcccta
    37921 ccgcattcgc cagaccggag ccacggagtt tggcctctgt cggccagatt tcagggacat
    37981 acactgccga ggcatagcaa acgtacatat agacggaagt aatcaggaag aaaccaatca
    38041 acgttatgag caacatactg gtttgcagtg aatagatata tccgagcacc gcaatcagga
    38101 ttaatagccc cacacccatt gttttacgcg gaattttatc catcaccagc atggcaataa
    38161 aaatactaaa tggcgcgcca aacatactca tggtatttaa aacaatcgag tcttttaaat
    38221 taatcccctg ggtcatgaat attgttggca accagttaat tagtgtgtac tgcacaacgt
    38281 tcatggcaat cagcacacaa gagcctaata tcacgcgttt cagtaatact cctgtcagta
    38341 aggctgaata cggcaccgct tgtggcgctt taccgtcatc agcaataacc acaggcggca
    38401 aaggtttacc cgtctggcgt atgacgcctt cttctatact ccgcatgact ttctcggctt
    38461 cctgataccg tccgcgcgat tccagccagc gcggggattc aggaaagtag cgccaggcca
    38521 gcgccgtagc gataagcgac aatattgcag ggataagcag ttgtacccgc cagttccact
    38581 ctgcactaat cagcggcgtg agtcccatcg caatcaatga acagagcgga tatgaccagt
    38641 tgccaataaa ggaaacccga ctggaccacg ttccgcgatt tctaccgggc atatattcgg
    38701 tgaaaccagc aaacagtgta accagtaaag ctcccagccc aacgcccatc acaaaacggc
    38761 aggcaataag aaaatccata ttcggtgaga aagcaccgac agccatcgag gcaatatgaa
    38821 tggcctcata gaggatgaac gcatttctgc gaccggtttt gtcgccaatg atgccgccca
    38881 ccagcgcacc gagaaacatt acggcggtcg tgattgctga gaatgtggct gtggtggaat
    38941 tatctgtcca gcccaacgct ttcagctgcg cgaggatcaa gccaccaacg gcattactcc
    39001 agcagacaag caagccaaac gcgacgatgg caaacattga cgaatgccag cggcaatccg
    39061 gtaagcgatc cagccgtgca ccacaatgcg gttttgttat ctgttccatg cgataacgtt
    39121 ccttcaggag agtggttatt catcaaagtc gtaagtcatg atcactttga ttgcggtttt
    39181 atcgaccatc gcatcaaacc cttcgcgcca ttgcgacagg ccgatacgat gcgtgatcat
    39241 cggtttgact ttgatagcgc cgctggctaa tagcctgata gcgttacgcc atgaggtgga
    39301 gtcataggcc atatgcccaa tgatgctttt attccaggcg gtaatgtcat taatcgagaa
    39361 atcaagaggt ttgaagccca ttccaacgcg taccacttcc ccattcgggc ggagcatttc
    39421 gatggcttgt ttcagtgcga tattggcacc ggagcattca atcaccagtc ccagattatc
    39481 tttgccacaa atttgctggc agcgcgccac cacatcttcg gtagaaccat ttactactgc
    39541 cgtcgccccc agttcttttg caaccgggaa gcggaccgcc acatcttctt gcagaccaac
    39601 gacgacgata tttaccgccc ccataatccg cgccatttgt acggaaaaca gcccgagtgg
    39661 gccagtgccg atgacgacaa catcctgacc aggaaggaat ttcgattgct gcgcgatgga
    39721 tttataggca ttacagatag ggtcaagtac ggctgcgtcc tcataatcaa caccatcagg
    39781 gatttcccac aacgcatgac gatgaatttt gagaatttca ccaggaacca gacaatattt
    39841 ggaaaaacca ccgccccagg tattgttatc cagaccaagg tttacttttt ctgtacaaca
    39901 cagaaaatca ccctgttcac aggccggaca aacgccgcaa acgtgaccgc tgttatccga
    39961 cacgacgcgt tgccccactt tccagtcttt gactttttca ccaacctgcg caatacaacc
    40021 tgcgaactca tggccgcgga tagagttaaa ctcatcagaa ccgctatcga cattgtagtg
    40081 cttcatgtct gcgccgcaga ttgccgcggc tttaatttca atcactacgt cttccgggcc
    40141 acaaatgggt tgtgggacat caatcatctt gtagccgcca aaggccttgc caaaccgagc
    40201 cagtgctttc attgcgtcat cctctgtgtt ttgttttgct tagatggcca tcatacttac
    40261 ccacatgatt tttgaggtca acattaaatc tgtaaaacac agataatgat ctgcgtttta
    40321 caacttagat cacaattacg cagaaaatgg ctgggtctgt tacaggttga tggaaggcgg
    40381 gacgcaaaaa gagcaaattc gagagggatt accggccaaa taggtctgag acttaagttc
    40441 aggagaagtc attcaggcgt gaacgcgacg gcaagagatc ggactgggac ttagccaggt
    40501 cggggcgaga taattgagac gagtcgtggg gtcattgttg gctatcccct tcatagccgc
    40561 ggcaaaaagg ggataaacca cttacttaaa catagcaata ggctttgctg agataccaaa
    40621 atcttctttt aactgctgtt tgctcttcat gaccatctga ccatttgtgt caattgtcat
    40681 atgctgtgct tcggtattat ggcgggcttg ccacagcatc accagttgca ggcaattctc
    40741 tttttgttcc tcggtcaacg caacgccatc aggccatttc cccagttcaa cggcggtcga
    40801 caaacgctgg tatacctcag gcgtcatgct gttgataatg tcatcaagat tcataatttt
    40861 tccgctcctg tggaataagt tgctgaatcg ttttttcaac cgttgatttc ttcgccgttt
    40921 tcgccatcag tgaagcgtaa agaagcagag ttaacacaat aacgttcgcc tgttggttgc
    40981 ggcccgtcgg gaaagacatg ccccagatgg gcatcacagt taccgcaacg aatttctatg
    41041 cgctgcattc catgtgacaa gtctttgata taacgaatgg attcttcact caccggttcg
    41101 tagaaactgg gccagccaca gccggaatca tacttggttt cggaatgaaa cagcggggca
    41161 tcgcagatca aacagtgata tacgccgtca cgcttgttat gcagtaaacg acccgtaaat
    41221 ggcggttctg tcccatgatt ctgcgtcacg taaaactgca tctcggacaa attttttttc
    41281 agttcttctg ccgaaggttt attagccatt tgctcacatc tcactttaat cgtgctcaca
    41341 ttacgtgact gattctaaca aaacattaac accaactggc aaaattttgt cctaaacttg
    41401 atctcgacga aatggctgca cctaaatcgt gatgaaaatc acatttttat cgtaattgcc
    41461 ctttaaaatt cgggacgccg accccatgtg gtttcaagcc caaaggaaga gtgaggcgag
    41521 tcagtcgcgt aatgcttagg cacaggattg atttgtcgca atgattgaca cgattccgct
    41581 tgacgctgcg taaggttttt gtaattttac aggcaacctt ttattcacta acaaatagct
    41641 ggtggaatat atgactatca aagtaggtat caacggtttt ggccgtatcg gtcgcattgt
    41701 tttccgtgct gctcagaaac gttctgacat cgagatcgtt gcaatcaacg acctgttaga
    41761 cgctgattac atggcataca tgctgaaata tgactccact cacggccgtt tcgacggtac
    41821 cgttgaagtg aaagacggtc atctgatcgt taacggtaaa aaaatccgtg ttaccgctga
    41881 acgtgatccg gctaacctga aatgggacga agttggtgtt gacgttgtcg ctgaagcaac
    41941 tggtctgttc ctgactgacg aaactgctcg taaacacatc accgctggtg cgaagaaagt
    42001 ggttatgact ggtccgtcta aagacaacac tccgatgttc gttaaaggcg ctaacttcga
    42061 caaatatgct ggccaggaca tcgtttccaa cgcttcctgc accaccaact gcctggctcc
    42121 gctggctaaa gttatcaacg ataacttcgg catcatcgaa ggtctgatga ccaccgttca
    42181 cgctactacc gctactcaga aaaccgttga tggcccgtct cacaaagact ggcgcggcgg
    42241 ccgcggcgct tcccagaaca tcattccgtc ctctaccggt gctgctaaag ctgtaggtaa
    42301 agtactgcca gaactgaatg gcaaactgac tggtatggcg ttccgcgttc cgaccccgaa
    42361 cgtatccgta gttgacctga ccgttcgtct ggaaaaagct gcaacttacg agcagatcaa
    42421 agctgccgtt aaagctgctg ctgaaggcga aatgaaaggc gttctgggct acaccgaaga
    42481 tgacgtagta tctaccgatt tcaacggcga agtttgcact tccgtgttcg atgctaaagc
    42541 tggtatcgct ctgaacgaca acttcgtgaa actggtatcc tggtacgaca acgaaaccgg
    42601 ttactccaat aaagttctgg acctgatcgc tcacatctcc aaataagttg agatgacact
    42661 gtgatctaaa aagagcgact tcggtcgctc ttttttttac ctgataaaat gaagttaaag
    42721 gactgcgtca tgattaagaa aatttttgcc cttccggtca tcgaacaaat ctcccctgtc
    42781 ctctcccgtc gtaaactgga tgaactggac ctcattgtgg tcgatcatcc ccaggtaaaa
    42841 gcctcttttg cattacaggg cgcacacctt ctctcgtgga aacctgcggg tgaagaagaa
    42901 gttctgtggt tgagcaacaa cacaccgttc aaaaatggcg tcgctattcg cggtggcgta
    42961 ccggtttgct ggccgtggtt tggtccggcg gcacaacaag gtctgcctgc gcacggtttt
    43021 gcccgcaacc tgccgtggac gctgaaatca catcatgaag atgctgatgg cgtagcgctg
    43081 acttttgaat tgacacaaag cgaagagacg aaaaaattct ggccgcacga ctttacgctg
    43141 ttagcgcatt tccgcgtggg taaaacctgt gaaatcgatc ttgaatcaca tggcgaattt
    43201 gaaaccacct ctgccctgca tacctacttt aacgtgggtg atatcgctaa ggtaaacgtc
    43261 agtgggctgg gcgatcgctt cattgataaa gtgaatgacg cgaaagaaga tgtactgacc
    43321 gatggtattc agaccttccc tgaccgtacc gatcgcgtgt atctgaatcc acaagattgc
    43381 agcgtgatta atgatgaagc gctgaatcgt attatcgccg taggccacca gcatcatctg
    43441 aacgttgtcg gctggaaccc gggaccggcg ctttcaatta gcatgggcga tatgccggat
    43501 gatggctaca aaacatttgt ttgtgtagaa acggcttacg cttcagaaac gcaaaaagtg
    43561 accaaagaga aacctgcaca tctggcgcaa tccattcgcg ttgcgaaacg ttaatttgcg
    43621 ttaatgttgt gtgccgggtg cgatgcatcc ggcacgtatc aacatcacac catatccagc
    43681 gcagtttttc cttttggtgc cggatatgct ttatccagca tggttaattc cgctgacgaa
    43741 agttcgacct caagcacagc cgcattctgt tggacatggg caatcgtggc cgcttttgga
    43801 atcgccatca caccatgatg gctgattacc cacgccaaca atacttgtgc cgcgctgata
    43861 ttgtgagcat gtgcaatttc gttgactacc gcgtttttta acagtccatt gcgcaaccgc
    43921 ccggcctggg ctaatggact gtaagccatt accgacatct gctgttgctg gcaccagggg
    43981 agtagatcgt actcaatccc tcgtgaaccg agatggtaaa gcacctgatt agtggcacac
    44041 tgatttcccc ccggcagctg ccagagttcc tgcatatcag cataatcaag gttagaaacg
    44101 ccccagcggc ggatttttcc ctgggcgatc aatttttcca tcgctgcaac agtctcttca
    44161 aaagcgaaac tgccagacca gtgtaataag tagagatcga gataatcagt attgagacgg
    44221 cgtaaactgg cttcgcatgc atttatcgct ttttgcccgc cagcattcca cggatagact
    44281 ttagagacga gaaagacgtt atcgcgcaga ccggttaatg cttccccaac caccttttcg
    44341 gcaccgccat cggcatacat ttcggcggta tcaatgaggg ttaaaccgag ttcaatgccc
    44401 gcgcgtagtg cagcaacttc tgttttgcgc tgactggcat cttcgcccat ataccatgtt
    44461 ccctgcccta tggctggcag tgagacatcg ccactaaatt gaatcatttt ttgttgcatt
    44521 gtttcctccc aggtattgca ccaccgtaat gcaaaacagg gcgtgacgcc ctgtttttat
    44581 gcacaaaatg ccctggaaag atgcattatc agaatttgta ggtgatcccg gtagaaatca
    44641 ggccagtcca ggatttatcc accatcgggc tgtcagtaac ttcatcagac agacgggtat
    44701 agcgcgcggt accgtaaaca ctccagtcgc cgaggaagtt gtagctggcg ctcagctcca
    44761 ggtaagggct ccagctgtcg ttcgggttat agccacgcag accgctgcga gcggactctt
    44821 tgcgcgatac gccataatag tattcgttct ggttttcgct gttccactgc acaccgatac
    44881 ccggggtcac ggtcaggccg ccgttggtgt aacgatacaa ccaggccata tcccagacaa
    44941 tgccgttgct gttatccagg gtatcgccag ccagggtggt acgcaggtaa ccgtactggg
    45001 taaagtgagc ataagacaga ccagccatca tggtgctctt acggtcatcc aggtgacgca
    45061 tttggtgatc gccactgtct ttcgctttga agtaaagcgg cgaccagtaa gcggtaattg
    45121 aaagtttatc cgttgcgtca ttccacaggt agtaaccacc acctaagcca cggaaccaga
    45181 agttatcgcc ttcatagttg attaccggta ctgggtaaac atcggtatcg taatctttat
    45241 atgggtgttc aacgacacct acgcctgcgc ccaaggaaaa tttaccttca gcgtgcgcta
    45301 cgcctgcaga cgttgcgata agcactccaa gtgccagaag tttgagtttg gtcacaatta
    45361 atcattcctt aaacaaatgt ttagcgggcg acaaagttta cccgtcaata catcgaaacc
    45421 caaccttttt acgttttcat tttttaaagt aactgtttaa ttttcctgac gcggatgaca
    45481 ccgcgcttac agccaaatga atttagcgtt actggctacc ctggtcttta cattaattat
    45541 gcaaaattta tggatgagtt gttgatatgc cattgaaatt aagaaagccg tgcaggcaag
    45601 ttttccattt gccatctacg cttaattttg aaggtgtatc accgggcacg ttgttctcat
    45661 cgtcgataaa atggcatgag agttgctgtg ttttagcaag agacgtcgtt cagtttacct
    45721 cttccgggag cctctactat tcatatgaac ggctcttaac ctgtgctaaa aaacgaaagg
    45781 acggcatacc atgaatatat tcgatcacta tcgccagcga tatgaagctg ccaaggacga
    45841 agagttcaca ctgcaggagt ttcttaccac ttgtcggcaa gatcgcagtg cttatgccaa
    45901 cgcggctgag cggctattga tggctatcgg tgagcctgtc atggtcgata cagcccagga
    45961 acccagactt tctcgactct tttctaaccg ggtcattgca cgttatccgg cgtttgaaga
    46021 gttttacggc atggaagacg cgattgaaca gattgtctct tatctgaaac acgcggctca
    46081 ggggctggaa gagaagaaac aaatcctgta tctgctgggg cctgtgggtg ggggtaaatc
    46141 atcgcttgct gagcgactga aatcattaat gcagctcgta ccgatttatg tattgagcgc
    46201 caacggtgag cgtagcccgg tcaacgatca tccgttctgt cttttcaatc cgcaggaaga
    46261 tgctcagatt ctggaaaaag agtatggcat tcctcgccgt tatctcggca ccatcatgtc
    46321 gccatgggcg gcaaaacgcc tgcatgaatt tggtggcgat atcactaagt tccgggtagt
    46381 gaaggtctgg ccgtcaattc tgcaacaaat tgctatcgcc aaaacggaac ccggtgatga
    46441 gaacaaccag gacatctccg cgctggttgg gaaagtcgat attcgtaaac tcgaacacta
    46501 cgcgcagaat gacccggacg cctacggcta ttccggtgcg ctgtgccgcg ccaaccaagg
    46561 gatcatggag ttcgttgaga tgtttaaagc accgataaaa gtgctgcatc cgctgttaac
    46621 cgccactcag gaaggtaact acaacgggac ggaaggtatc tccgccctgc cgttcaacgg
    46681 gattatcctc gcccactcga acgaatccga atgggtcact ttccgtaata acaaaaacaa
    46741 cgaagccttt ctcgaccgtg tttacatcgt gaaggtgccg tattgcttgc gcatttccga
    46801 agagatcaaa atctacgaga aattgcttaa tcacagtgaa ttgactcacg ccccctgcgc
    46861 ccctggcacg ctcgaaacac tgtcacgttt ttccattctt tcgcgcctga aagagccaga
    46921 aaactccagc atttattcaa agatgcgggt ttatgatggc gaaagtctga aagacaccga
    46981 tcccaaagcc aagtcgtatc aggaatatcg tgactacgcc ggtgtcgatg aagggatgaa
    47041 cggtctgtcg acgcgttttg cgtttaagat cctctcccgc gtgttcaact tcgatcatgt
    47101 agaagtggcg gcaaacccag tccatctgtt ctacgtcctg gaacagcaga tcgagcgcga
    47161 gcagttccca caagagcagg cagaacgcta tctggagttc ctgaaaggtt atctgatccc
    47221 gaaatatgcc gagtttatcg gcaaagagat ccagacggcc taccttgaat cctattccga
    47281 atatgggcaa aacattttcg accgttatgt tacctacgcg gatttctgga ttcaggatca
    47341 ggagtatcgc gatccggata ccgggcagct gtttgaccgc gagtctctta acgccgagct
    47401 ggagaaaatc gagaaaccgg cggggatcag taatccaaaa gatttccgca acgagattgt
    47461 taacttcgta ctgcgcgcca gagcgaataa cagcggacgc aatccgaact ggaccagcta
    47521 tgaaaaactg cgcacggtta tcgagaagaa aatgttctcc aataccgagg agctgttgcc
    47581 ggttatctcg tttaacgcca aaacgtcaac cgacgagcag aggaaacacg acgactttgt
    47641 cgaccgtatg atggaaaaag gctacacccg taaacaggtg cgtttactgt gcgaatggta
    47701 tttgcgcgta cgtaaatcgt cttaacaacc ctggcccggt acacatgcta ccgggcctac
    47761 aacgacagcg aaccgtgggc cagagaaatg gcaacacagg cgtagcatac agttggcaaa
    47821 tgtagtacgg ggggcatatg acctggttta ttgaccggcg tctgaacggc aaaaacaaaa
    47881 gcatggtgaa tcgccagcgt tttttacgcc gttataaagc gcaaattaaa cagtcgatct
    47941 ccgaggccat taataagcgt tcggtgactg acgtcgacag cggcgaatcc gtatccattc
    48001 ccacggaaga tattagcgaa ccaatgtttc atcaggggcg tggcggcctg cgccaccgcg
    48061 tacatccggg caatgaccat ttcgtccaga acgaccgaat tgaacgtccc cagggtggcg
    48121 gcggaggttc cggcagtggt cagggccagg ccagccagga tggtgaaggt caggatgaat
    48181 ttgtctttca gatttcgaaa gatgagtatc ttgatctgct ctttgaagat ttggccttac
    48241 cgaatctgaa acaaaaccaa caacgccagc tgaccgaata taaaacgcat cgggcgggtt
    48301 ataccgctaa cggcgttcca gccaatatca gcgttgtgcg ttccttgcag aactcactgg
    48361 cgcgacgcac agccatgacg gcgggcaagc gacgggaact tcatgcgctg gaagagaatt
    48421 tggccatcat cagcaacagt gaacctgcgc aactgctgga agaggaacgt ctgcgcaaag
    48481 aaattgcaga attacgtgcc aaaattgaac gcgtcccttt tattgacacc ttcgatttac
    48541 gttacaagaa ctacgagaag cggcccgatc cctccagcca ggcagtgatg ttttgcctga
    48601 tggacgtttc cggttcaatg gatcaatcca ctaaagatat ggctaagcgt ttttatattc
    48661 tgctgtatct gttcctcagc agaacgtata agaacgtgga agtcgtatac atccgccatc
    48721 atacccaggc gaaagaagtc gatgaacatg agtttttcta ctcgcaggaa acaggcggca
    48781 ccattgtttc cagcgccctg aaactgatgg atgaggtagt gaaagagcgt tataacccgg
    48841 cacagtggaa tatttacgct gcacaagcat cggacggcga taactgggcc gatgactctc
    48901 cgctttgcca tgaaatcctg gcgaaaaaat tattacctgt tgttcgttat tacagctata
    48961 tcgaaattac ccgtcgtgca catcagacat tgtggcgaga atatgagcat ctgcaatcta
    49021 ctttcgacaa ctttgcgatg cagcacatcc gcgaccagga tgatatttat ccggtgttcc
    49081 gtgaactgtt tcataaacaa aatgcaacag ctaaagacta aaactatcag ccaggtcatt
    49141 atcgcctggc tgatttttag cttactgtaa attatctcat ttattacata cattagctta
    49201 caatcgcttt aaatatgaca gcataacctt tacataattt agttccagaa aacaatcatt
    49261 cggaaaaatg attcagtcaa cacgtatttc catggggtta ttctttaaat attttttatc
    49321 gttaacgaaa attgatcctg gtcaaaacta tatatctctg ccatcaataa aatccagcac
    49381 tcacattgct ctccttttta tggtttctat gggtacacaa aaattaaaag ctcaaagctt
    49441 ttttattttc agtctattgc tgacgttaat tttattttgc attactacct tatataacga
    49501 aaacacaaat gtaaaactca tcccacagat gaattacctg atggttgttg tggctttgtt
    49561 tttccttaac gccgtcattt ttcttttcat gttaatgaaa tatttcacta acaaacaaat
    49621 tttaccaata ctcattttaa gccttgcatt tttaagtggc cttatctatt tagttgaaac
    49681 cattgtaatt atccataaac caattaacgg cagtacactg atccagacaa agtcgaatga
    49741 tgtttctatt ttctatattt tccgccaact cagttttatt tgtttaacct cgctggcact
    49801 cttttgttat ggaaaagaca acatccttga caacaataag aaaaaaacgg gaatcctgtt
    49861 gctggcgctg attccttttt tagtttttcc ccttctggca cacaatctga gcagttataa
    49921 cgctgactat tctttgtatg tcgtcgacta ctgtccggac aaccatactg cgacctgggg
    49981 aatcaactat acaaaaatat tggtttgtct gtgggcattt ttactgttct ttattatcat
    50041 gcgtacacga ttagccagcg aactatggcc gttaatagca ttattatgtc tggcatcgct
    50101 atgctgcaac ttacttctac tgactctgga tgagtataat tacaccatct ggtatatcag
    50161 tcgcgggatt gaagtttcca gtaaactgtt tgttgtgtct tttctgattt ataacatttt
    50221 tcaggaactg caactctcca gcaaactggc agttcatgat gtgctgacca atatttataa
    50281 tcggcgctac tttttcaaca gcgtagagtc attattgtcg cgacctgttg ttaaggactt
    50341 ctgtgtcatg ctggttgata ttaatcagtt caaacgcatc aatgcccaat ggggacatcg
    50401 tgtgggtgat aaagtgctgg tttcaattgt cgatattatc cagcaaagca tccgccccga
    50461 tgatatttta gcgcgactgg agggtgaggt gtttggcttg ctatttaccg aactcaatag
    50521 tgcccaggca aaaatcattg cggaacgtat gcgtaaaaat gtcgaactcc tgaccggctt
    50581 tagtaacaga tatgatgttc ctgaacaaat gaccatcagt attggcacgg ttttttcaac
    50641 gggtgacacg cgtaatatct cgcttgtcat gacggaagca gataaagcct tacgcgaagc
    50701 gaaaagcgag gggggcaaca aagtgattat tcatcatatt taagtgcaaa aatattcaga
    50761 gccatgcttt ttgcgtggct tttgcataca atttattact attaccccta aatttcccct
    50821 ccccagcggc gcagagatga gtataattag cgcccctgtg ccaggccgca atcgaacttt
    50881 atctggtttt ctcgtttcac taaccgaagg agtgccattt atcatgaaat tgcaccatag
    50941 aatgctccgg cattttatcg ccgcaagtgt cattgtgctg acatcttcct tccttatttt
    51001 tgaacttgtc gccagcgaca gagcaatgag tgcctatctg cgctatatcg tgcagaaagc
    51061 agactcctcc tttctttatg ataagtatca gaatcagagt attgccgcgc atgtgatgcg
    51121 cgctctcgct gctgagcagt cggaagtgtc gccagaacag cggcgcacca tctgcgaggc
    51181 ttttgagtct gccaataata cccatggctt aaacctgact gcccataaat acccgggctt
    51241 acgcggcaca ctacaaaccg catccactga ctgcgacaca attgtggaag ctgcagcact
    51301 attacccgct tttgatcagg cagtggaagg caaccgccac caggatgatt acggttcagg
    51361 tcttgggatg gccgaagaga aatttcacta ttatctcgat ctcaatgacc gctatgtcta
    51421 tttttatgag ccggttaatg ttgaatactt tgcgatgaat aactggtcct tcctgcagtc
    51481 aggaagtatt ggcatcgatc gcaaagatat tgaaaaggta tttaccgggc gtaccgtatt
    51541 gtcgagcatt taccaggatc agcgtactaa acagaacgtg atgagtttgc tgacgccggt
    51601 atatgtcgca gggcagctaa aagggattgt gctgctggat attaacaaaa acaatttgcg
    51661 gaatatcttt tacactcatg accgccctct cctctggcgt tttctcaatg tcacgctaac
    51721 cgataccgat tcggggcgcg acattatcat caaccagagc gaagataatc tgttccagta
    51781 tgtcagttac gtccatgact taccgggcgg cattcgtgtc tcgttatcca ttgatattct
    51841 ttactttatc acgtcttcgt ggaaaagcgt tctgttctgg attttgacgg cgttaatttt
    51901 gctgaatatg gtccggatgc acttccgttt ataccaaaat gtgtcgcgag aaaatattag
    51961 tgatgcgatg actggactgt ataatcgcaa aattttaacc cctgaactgg agcagcggtt
    52021 gcagaaactg gtgcaatccg gttcttcggt gatgtttatt gcaattgaca tggacaagtt
    52081 aaagcaaata aatgacaccc tcggtcatca ggagggggat ttagcgatta cgttattagc
    52141 tcaggcgatt aaacaatcga ttcgtaaaag tgattatgcc atccgactcg gtggcgatga
    52201 attctgcatc attcttgtcg attcgacgcc gcaaattgca gcacaactgc ctgaacgtat
    52261 cgaaaaacgt ctgcaacata tcgcgccgca gaaagaaatc ggcttctctt ccggtattta
    52321 cgcgatgaaa gaaaacgata cgttacatga tgcgtataaa gcttccgatg agcgtttata
    52381 tgtcaataag cagaacaaaa acagccgttc atgataacct tctgtggttg tttgcttgta
    52441 atctcaggag cgtgaaatga ctgaaatggc taaaggaagc gtgacgcatc agcggttaat
    52501 cgctttatta tcacaagaag gtgctaactt ccgcgtcgtt acccatgaag cggtaggtaa
    52561 atgtgaagcg gtgtcagaaa ttcgtggcac cgctctaggc cagggtgcta aggcactggt
    52621 atgtaaagtc aaaggcaatg gcgtaaatca acatgttctg gcgatcctcg ctgccgatca
    52681 acaggccgat ctgagccaac tcgccagcca tataggcgga ttacgcgctt cgttggccag
    52741 cccggcggaa gtcgatgaac tcacaggctg tgtcttcggc gcgatccccc ctttcagctt
    52801 ccatccaaaa ctcaaactgg ttgccgaccc tctactcttc gaacgatttg atgaaatcgc
    52861 cttcaatgca ggcatgctgg ataaatccgt tattttgaaa accgcggatt atttgcgcat
    52921 tgcgcaacca gaactggtca atttccgccg cactgcgtaa ctagcctgtc cgttcaataa
    52981 gcagaacgga caaaaccagc acaacagcaa tggcaaaaaa cgatgacgtg ataatcagtg
    53041 tctcgacgaa catttgatcg ttcatagtat gcccccggga gatagctgtt ttcacgttat
    53101 tgttagcgtg cacaaatggc agtttgatga cagttcgcct gattttttat gcaaaaaacg
    53161 taaagatttt ctacttcctt cctgcagcaa gcgtaaagta agcaggctta ttattttttg
    53221 gcaaggaaac cacgatgttt gatgccactt tgctgatcct gctcgggtta gctgcgctgg
    53281 gctttatcag tcataacacc actgtcgccg tttcaattct ggtgttgatc attgtccgcg
    53341 tcacaccgtt aagcaccttt ttcccgtgga ttgaaaaaca ggggctaagt atcgggataa
    53401 tcattctgac gattggcgtt atggccccca tcgccagcgg gacgctaccg ccctcaacct
    53461 tgattcactc gtttctgaac tggaaatcgc tggtggcaat tgcagtaggc gttattgtct
    53521 cctggctggg tgggcgcggc gtgaccttaa tgggcagcca gccgcaactg gtcgccgggc
    53581 tgctggtagg cactgtttta ggcgtggcgc tgtttcgcgg cgtaccggtc ggcccgctta
    53641 ttgccgcggg tttggtttcg ctgattgtgg ggaaacagta gttaatcccg caatatatcg
    53701 gcctggcgtt tgccccaggc ctttcttaaa catagtaata aaggccgttg ttgaatcata
    53761 ccccagcgta tgagcaactt tctgtacagt atcgcctttc actaatccct gaagtgccat
    53821 aatcagctgt aactgctgac gccactggcg aaaactcaac cccgtctctt ttactatcag
    53881 tcgcgcaaga ttgcgttcac tcatcgcaaa aaagccagcc cattgcccta atgaccccca
    53941 ctcgacgggc cctttcgcca tcatctccac catcgtgcgg attttaggat gagaagaaac
    54001 gggctggtgc agctgttgtt gtggctgctg agggagttca tcaaacagga cctgaattaa
    54061 tctgcgggtc atcggctcag cacgctgagt cgtcgtcctg tttgccaggg ttaaaatcaa
    54121 ttcccggcat aagggcgata ttttcaatgt gcaacatgtt gtcggcattg tcactgcgct
    54181 gggttcgata aacagaaaac agagttcagc gttagctgtc acttgattgc tatgttccac
    54241 cccgcccggt atccaaaccg catattgcgg cggcaccatc cacaaagcat tttccaccgt
    54301 acaggtaatt gcaccatgta atgctagtat cagctgtcct ttgcggtgct gatgtacagg
    54361 actggttaac tcatccgttc cggcgtgaat acaaaatgcg accgcggctt catggtgtcg
    54421 gtcgggttca tagccattga gattcaacct gtgcatcatt ttgtccgaat ttagcgataa
    54481 tttgtcattt tagcttgatt caacataaca ataaaaacgg taaggtacag cctcgtttgt
    54541 aacaatgaga agcatatgac ctgttcaact tcattaagcg gcaaaaacag gattgtcctt
    54601 atcgctggca ttctgatgat tgccacaaca ttacgcgtca cctttaccgg cgcagcgccg
    54661 ttactggata cgattcgttc cgcttactcg ctgacgacag cgcaaaccgg cttattaacc
    54721 accctgccat tattggcctt tgcgctaatc tcacctttgg ctgccccggt ggcgcgacgt
    54781 tttggtatgg aacgtagcct gtttgccgcg ttacttttga tctgtgcagg tatcgcaatt
    54841 cgttctctcc cttcgcctta cttattattt ggcggtacag cggtcattgg cggtgggatt
    54901 gcattaggca atgtcttact gccaggatta attaaacgcg atttccctca ttccgtcgcc
    54961 agacttaccg gcgcatattc cctgacaatg ggagctgcag cggcactggg atcggctatg
    55021 gtcgtgccgc tggctttgaa cggttttggc tggcaaggcg cgttgttcat gctgatgtgt
    55081 tttcctctgc tggctctttt tttatggctg ccacagtggc gaagccaaca acatgcaaat
    55141 ttgagtacct cgcgcgcctt acatactcgg ggtatctggc gttcgccgct tgcctggcag
    55201 gtcacattgt ttcttgggat caactcactg gtctattacg tgattattgg ctggcttccg
    55261 gcgatcctca tcagtcacgg ctatagcgaa gcacaggcgg gttcactgca tggtttgctg
    55321 caactagcca cagcagctcc cggtttgctg atccctcttt tcttacatca tgtgaaagat
    55381 cagcgtggta ttgcagcgtt cgttgccttg atgtgcgcag tgggcgctgt tgggctctgc
    55441 tttatgccag cgcacgcgat cacctggact ctgcttttcg gttttggttc cggcgcaaca
    55501 atgatactgg ggttgacgtt cattggtctt cgggcaagtt ctgcgcatca ggcggcggca
    55561 ctctcgggga tggcacaatc cgtcgggtat ttgctggcag cctgtgggcc gccgctgatg
    55621 ggtaaaatac acgatgctaa cggtgactgg tccgtaccac tgatgggtgt tgcaatactt
    55681 tcactactga tggcgatttt cggactttgc gccgggagag acaaagaaat tcgctaatat
    55741 ccggtgctat agtgacgtga caaatcatgc gtgaaaggga gaacaaacac gatgaatatt
    55801 cagtgcaaac gcgtttatga tccggctgaa cagagcgatg gttatcgcgt actggtcgac
    55861 cgcctctggc cgcgcggtat caaaaaaacc gatttagccc ttgatgagtg ggataaagaa
    55921 atcacgccgt caacggaact gcgcaaagcc tttcacggcg aagtcgtcga ttttgcaacc
    55981 tttcgcgagc aatatcttgc agaactggcg caacacgagc aagaaggaaa gcggctggcg
    56041 gacatcgcca aaaaacagcc gctgaccctg ctctacgcag caaaaaacac cacgcagaac
    56101 catgcgctgg tgctggccga ctggctacgt agcttgtgat tttagtacag catccggcgg
    56161 ttatttttca ccagccggat ggtcccgtcg ccacaatgcc cattcatcaa tcgtttcacc
    56221 gcccggtaat ttgcaattgt tgctgacccc ttgcgctgtc gacactggaa tgagcgtccc
    56281 gcccttctgc tggcaataga ccgacgccgg atttgccata ccaatctgcg gcggtttagg
    56341 tgcttctggc tgagaagagg ttgaacaacc agccaggacc agcaagcaag gcaaagcaaa
    56401 actgataatt ttcatttatt gatctcactc atttatctac gcttagagta tcgcggtatc
    56461 gttttgtttt gcaacactat ttttattaca ttcactcaaa acatattacg tcttgtttca
    56521 tctttgttgg tgatgtttta tcatgcctgc aaagattaaa taatcagcat ttacccgccg
    56581 tatcctggag ttgtttcgtg tcagatcaga ttatcgcccg cgtctcgcaa tcccttgcca
    56641 aagaacagtc actggaaagt ctggtccgac agcttctgga gatgctggaa atggtcactg
    56701 atatggaatc aacctacctg accaaagtgg atgtcgaagc gcgcctgcag catattatgt
    56761 ttgcccgtaa cagccagaaa atgcacatcc cggagaattt taccgtctcg tgggattact
    56821 cgttatgcaa acgcgccatt gatgaaaact gctttttcag cgatgaagtc cctgaccgtt
    56881 ggggcgactg tattgcggca cgcaatcttg gcatcaccac atttctaagc acgccaattc
    56941 acttaccgga tggatcattc tatggcacgc tttgcgccgc cagcagtgag aagcgccagt
    57001 ggagtgaacg cgcggaacag gttttacagt tattcgccgg actgattgca caatatattc
    57061 aaaaagaggc actggttgaa cagctgcgcg aagccaatgc tgcactgatt gcgcaatcgt
    57121 ataccgactc gttaaccggg ttgccgaatc ggcgggcgat ttttgaaaat ctgacgacgc
    57181 tgttttctct cgcccggcat cttaaccata agataatgat cgcgtttatc gatctggata
    57241 acttcaaatt aatcaatgat cgttttggtc ataatagtgg cgatctgttt ctcattcagg
    57301 ttggcgagcg ccttaatacg ctccagcaaa atggcgaagt tattggtcgt ctcggcggtg
    57361 atgagttttt ggttgtttca ctaaacaacg agaatgcgga tatttcgtcg ctgcgagaac
    57421 gcattcagca gcaaatacgt ggagaatatc acttaggtga tgttgatttg tattatcccg
    57481 gtgccagtct tggcatagta gaagtcgatc ctgaaacaac cgatgcagac agtgccctgc
    57541 atgctgccga tattgcgatg tatcaggaga aaaaacacaa acagaaaaca ccttttgtcg
    57601 cgcattcagc gccacattgc tgaggcgtat tcacatcctt ttgattggtg ataacatgcg
    57661 aatcggtatt atttttccgg ttgtaatctt tattacagcg gtcgtatttt tagcatggtt
    57721 ttttattggc ggctatgctg ccccgggggc ataaagatga aaaaaacaac gattattatg
    57781 atgggtgtgg cgattattgt cgtactcggc actgagctgg gatggtggta acgtcacctc
    57841 taaaaaatag caaaggctgc ctgtgtgcag cctttgtgca atttaagcgt taacttttaa
    57901 tcttcctgta gataaatagc acgacaatcg caccaataac ggcaaccacg aagctgccaa
    57961 aattgaagcc atcgacttta ccaaagccaa acagcgtgct gatccatccg ccgactacgg
    58021 caccgactat ccccagcagg atagtcataa agaatccacc accatcttta cctggcatga
    58081 tccacttcgc cagaataccg gcaataagcc caaaaataat ccatgacaga atgcccattg
    58141 tttcctcact tatctgtttt gcattagcgg gttagtcgct gataaaaagc atagcacaac
    58201 atcgggaggg caagatttgt gacgagcatc acggaggatt tttttgcgat ggcgcagaaa
    58261 ttgcgccatc aacgatcagt gataattacc aaccacaaac atcatgttcg ttttccgtgt
    58321 cataagaacg cacggtattc accagatctt ttatcacttc agccgccact tctggcacca
    58381 gcaaagtcat cggcgtctct gtttcataat cgacagaaac gccattactg ttattggtga
    58441 cggtcacggt atacgttgct ttgcccatga ttcatttccc gttatgaatg actttccgtt
    58501 attgcgcacc ttccatcagg acttcaggag ccacgaagaa gtcaatgttg aaataagtat
    58561 cgtcagtcat ggcttcaatg ttgtgccact tttctggagg gaacaccgca aactgccccg
    58621 cttcgataag gatcacctga tcaggctctg cactgtgttc atcagcgtag ccgagatatt
    58681 tgaccgcccc atgcataacg gaaaggcgtg ggtaaacccc cgggcgcgtt cctttatcaa
    58741 gatgacgttc gaatattccg gcaggcgcag tttgtttatt ccagaaaggc gttgagcgcg
    58801 tatgaatata attctgtggg atttgcagca tccttttccc tccttcggtg aatgcgctga
    58861 aaacggttta ttccagccgt ttcagggtac gcctgataat ttgcatttta aataccattt
    58921 attggttact ttttagcacc atatcagaga aaaatcaggg aggattacat gtgggaagcc
    58981 cgcgacaacg cgggctgaat gtatcaggat tgcagcgtcg ccagtcgggc ggcgaaaccc
    59041 acgaacatca aaccaatcag tgagttgcca actttagcca gtttcttttt ggtacgtatg
    59101 tactgcgtga caaaagcacc agaaataatc aggaagctca aatagcagaa actcaccagt
    59161 tccagcgtag tcgccagaat aaagaatgaa atccccgtat gtggggcatt aacatcgata
    59221 aactgaacga aaaacgacac atagaacaaa atggctttcg gattagtcag gctcaaaatt
    59281 aacgcccgtt taaaaatagc accgtattgg ggctcatcgg atttggcctc gctattttta
    59341 cccttcaggg tcgcgtaaag aattttactc cccagataga gcaaataaaa cgcaccaaga
    59401 taacgtacaa tgttgaataa tatcggggtg gtcttaatta atgtcgccac tccagcccat
    59461 gccagaaaca tcaataccgc atcgccaata aatacaccgc aggccgcaag ataaccgcct
    59521 ttcataccgc tactgacgct atttttgagt acaaacaggg tatttggccc tggcaccaac
    59581 acaataaaaa tggccccaac cagataggtc cagtaattca gaaccccgta ttcagcgaac
    59641 acattaacct ctttaattat ctttcgatca tgcgcgatta aaggtgaata tgctaaccaa
    59701 tctgtagcgg cttagaaagg agaaaatcag gttttaaccc gatatcaacc cgataattga
    59761 ataattaatc aggcatgctc cagtgaaaaa ttcgggtagt gctcggcaaa atactggcgt
    59821 aaaaattcta ccgttatccg cactttcgct gacgtcgcca gccttgaaac ataaacggcc
    59881 cagacgttcg ctggctggta atattccggt agcacttgca ctcaatgacc actggcaatg
    59941 ttttcgctaa catcccacca ggaacgcaga gcaatcccct gcccatcgag acaccactga
    60001 tggacaattt ctccatgatt agaggacagt gggccggtga ctttaatcgc atggtggcct
    60061 tctttattgc gtaattgcca gacaccaaaa ggatgatcgc gctctttaat gaccaggcac
    60121 ggcaatgcag aaagatcggt taaatgtttc ggcgcaccat gttgcgcaat aaattccggc
    60181 gacgcgcaga gaatgcggta attagtcgcc agtttgcggg cgattaaatt aggtgcgatg
    60241 tcatcgccaa tgcgaatatc gagatcgaca ccttcattga ccaaatcgac caaccggtct
    60301 tccacatcaa aacgtaattc cagttgcgga tacgccttcg ccagcgctga taacgccgga
    60361 gccaccacct gtcgtccaaa accaaagctg ctgataatac gcagcatccc ctggggcacc
    60421 tgacgcacgt cagaaagttc gtccatcatc tgaccgacat cctgcaaaat ccgctgcgcc
    60481 cattcataaa tccgctctcc ttcttcggta atggtgacgc ggcgggtggt gcggtgtaac
    60541 aacaccacgt ttagcgtttg ctccagcaag gcgatgcgct tgctgacgaa cgccggtgaa
    60601 acgcccagtt cttccgccac ggcggcaaaa ccagcccgac gagccaccag cataaagacg
    60661 cgcaaatcat tcagcagcgg taaattattc atgattcgtg ttttatgttt caccagttac
    60721 ggggattaat tccttttcag tcaattatag gatggtgatg ttgtcaattt tgatggtcag
    60781 gaagtgagaa cccaatgatg aaaacgatgc gtattgctgc gatcccggga gacgggattg
    60841 gcaaagaagt ccttcctgaa gggattcgcg tgttacaggc tgccgctgag cgctggggct
    60901 tcgacttgag ttttgagcaa atggagtggg cgagctgcga gtattacagc catcacggta
    60961 aaatgatgcc ggacgactgg catgagcaac ttagccgttt cgacgccatc tattttggtg
    61021 ccgtcggctg gccggatacc gttccggacc atatttcgtt gtggggttcg ctgctgaaat
    61081 ttcgtcgtga attcgaccag tacgtcaacc tgcgcccggt tcgtctcttt cctggcgttc
    61141 cctgcccgct ggcgggaaaa cagcctggcg acatcgattt ttacgtggtc agggaaaaca
    61201 ccgaaggcga atattcctcg ctcggcggta gagtgaatga aggtacagag catgaagtcg
    61261 tcattcagga atcggtattt acgcgtcgtg gtgtcgatcg cattttgcgt tatgccttcg
    61321 aacttgcgca aagccgccca cgtaagacgc taacttctgc cactaaatcg aacggtttag
    61381 ccatcagcat gccgtactgg gatgagcgag tggaagcaat ggccgagaat tacccggaga
    61441 tccgctggga caagcagcat attgatattc tctgcgcgcg ttttgtgatg cagccggaac
    61501 gcttcgatgt ggtggtggcg tccaatttgt ttggcgatat ccttcccgat cttggcccgg
    61561 cctgcaccgg caccattggc attgccccat ccgctaatct gaatccggaa cgcactttcc
    61621 cgtcgctctt cgagcccgtc cacggttccg cgccggatat ctacgggaaa aatattgcta
    61681 accctatcgc cacgatttgg gccggggcaa tgatgctcga ttttctcggc aatggcgatg
    61741 agcgtttcca gcaagcgcat aacggtattc tggcagcaat tgaagaagtg attgctcacg
    61801 ggccgaaaac acctgatatg aaaggcaatg ccaccacgcc acaggttgcc gacgcgattt
    61861 gcaaaattat tttgcgttaa ggtcaaacca gtttatttga accgcgtcac tgacgcggtt
    61921 tttttattcg ttctttgcag taaataacct gcgtcatttc accttttatt gtttccgttt
    61981 cgtgttttat ggctttccgt attcttaatt gtttaattta tgtaacatgc aaataatcaa
    62041 tttgttaaac acaacataca actcattacc ctgcaaataa ttacaactgg caaaagccag
    62101 agacgggctt ttatgcccag gagatgactc atgatgagca atgttaagaa aaaagatgtg
    62161 ccgctgataa gcatcagcct ggtggccatt cttttcatcg cagctgcatt aagccttttc
    62221 ccacaacaat cggccgacgt ggccaacgca atatacactt ttgttacgcg tacgttaggt
    62281 tccgccgtac aggtattggt tttgctggca atgggactgg tgatttattt agccaccagt
    62341 aaatacggca atattcgtct tggcgaagga aaaccggaat acagcacgct ctcctggctg
    62401 tttatgttta tttgtgccgg tttaggttct tctacgcttt attggggggt tgctgaatgg
    62461 gcttattatc aaacacctgg attaaatatc gcaccgcgtt cacaacaggc actcgaattt
    62521 agcgttccct actctttctt ccactggggc atcagcgcct gggcaactta tacgctggcc
    62581 tcattaatca tggcttatca ctttcatgtg cggaaaaaca aaggtctgag tctttccggc
    62641 attattgccg cgattaccgg cgttcgcccg caaggcccat ggggaaaact ggttgatttg
    62701 atgttcctga tcgccactgt cggcgcactg actatttccc ttgttgtcac cgcagcaacc
    62761 tttactcgtg gactttccgc gctgaccggt ttacccgata acttcaccgt gcaggcattt
    62821 gtgatcctgc tttccggcgg cattttttgc ctaagctcgt ggattggtat caacaacggt
    62881 ttgcaacgtc tgagcaaaat ggttggctgg ggcgcgttcc tgctgccatt actggtgctg
    62941 attgtcggtc caaccgaatt tattaccaac agcatcatca atgccatcgg cctgaccacg
    63001 caaaacttcc tgcaaatgag cttattcacc gatccgcttg gcgattgttc atttacccgc
    63061 aactggaccg ttttctactg gctgtggtgg atctcataca cccctggcgt agcaatgttt
    63121 gtcacccgcg tttcccgcgg tcgtaagatt aaagaagtta tctggggact gatcctcggc
    63181 agcaccgtcg gttgctggtt cttctttggc gtaatggaaa gctatgccat tcatcagttt
    63241 atcaatggcg taatcaacgt cccacaggtg ctggaaacac tgggcggcga aacagctgtg
    63301 cagcaagttc tgatgtcgtt gccagccggt aaattgttcc tcgccgcata cctgggtgtg
    63361 atgattattt tccttgcctc gcatatggat gcggtggcct acaccatggc tgcgaccagt
    63421 acgcgtaatc tccaggaagg tgacgatcct gaccgtgggc tgcgtctttt ctggtgcgtg
    63481 gtgatcactc tgatcccgct ttccatcttg tttaccggtg cttcgctgga aacgatgaaa
    63541 accaccgtcg tgctcacagc ccttcccttc ctcgtcattt tactggtgaa agtcggcgga
    63601 tttattcgct ggctgaaaca ggattacgcc gacattccgg ctcatcaagt tgaacattat
    63661 ctcccgcaga caccggttga agccctggaa aaaacaccag tgctccctgc gggaaccgta
    63721 ttcaaaggcg acaactgagc gccatcatcc taacgataaa ggtatcccta tgagcaatct
    63781 gagccctgac tttgtactac ccgaaaattt ttgcgctaac ccgcaagagg cgtggaccat
    63841 tcctgcccgt ttttataccg atcagaacgc gtttgaacac gaaaaagaga acgtcttcgc
    63901 caaaagctgg atttgcgtcg ctcacagcag cgaactggcg aatgccaatg attatgtgac
    63961 gcgtgagatc attggcgaaa gcatcgtgct ggtacgcggt cgtgataagg ttttgcgcgc
    64021 gttctataac gtgtgtccgc accgtggtca tcagttgttg agcggtgaag gaaaagcaaa
    64081 aaatgtgatt acctgcccgt atcacgcatg ggcattcaaa ctcgatggca atctggccca
    64141 tgcacgtaac tgcgaaaacg tcgccaattt cgatagcgac aaagcgcaac tggttccggt
    64201 gcgtctggaa gaatatgccg gattcgtctt catcaacatg gaccccaacg ccaccagcgt
    64261 agaagatcag ttacccggtc tgggcgcgaa agtgctggaa gcctgcccgg aagtccacga
    64321 tctgaaactg gcggcccgct ttaccacccg cacgcctgcc aactggaaga acattgtcga
    64381 taactatctc gagtgctatc actgtggtcc ggcgcatcca ggtttctccg actccgtaca
    64441 ggttgatcgt tactggcaca ccatgcacgg taactggacg ctgcaatacg gtttcgccaa
    64501 accgtccgaa cagtcgttta aatttgaaga gggtacggat gcggcattcc acggtttctg
    64561 gctgtggccg tgcacgatgc tgaacgtcac cccgatcaaa gggatgatga cggtcattta
    64621 tgaattcccg gtggattctg aaactaccct gcaaaactac gatatttact tcaccaatga
    64681 agagttaacc gacgagcaaa aatcgctgat tgagtggtat cgcgatgtgt tccgtccgga
    64741 agatttacgt ctggttgaaa gcgtacagaa agggctgaaa tcgcgtggct atcgtggtca
    64801 ggggcgcatc atggccgaca gtagcggtag tggcatttcc gaacatggta tcgcccattt
    64861 ccataatctg ctggcgcagg tgtttaagga ctaatgacat cggcggcggt attttccgcc
    64921 gctgggctga tttttgatgg agtacagcaa tgtcagacta tcaaatgttt gaagtacagg
    64981 tgagtcaggt ggaaccgctt accgaacagg tgaaacgctt cacgctggtg gcaaccgatg
    65041 gcaaaccgct gcctgcgttc accggaggaa gtcacatcat tgtgcagatg agcgatggtg
    65101 ataaccagta cagcaatgcg tattcactac tgagttcgcc gcatgacacc tcttgttatc
    65161 agattgccgt tcggctggag gaaaactcgc gcggcggttc ccgctttttg catcagcagg
    65221 taaaagtggg cgatcggtta acgatttcaa cgcctaataa cctgtttgcg ctaattccct
    65281 cagccagaaa gcatctgttt atcgcgggcg gtattggtat cacccctttc ctgtcgcaca
    65341 tggcagagct gcaacacagc gacgtcgact ggcagctaca ttactgctcg cgaaatccag
    65401 aaagttgcgc atttcgtgat gagctagtcc agcatccgca ggctgagaaa gtccatttgc
    65461 atcattcatc aaccggaaca cgactggaat tagcgcgatt attggcggat atcgaacctg
    65521 gcacacacgt ttatacctgt ggccccgagg cgctaattga agcggtaaga agtgaagctg
    65581 cgcgtctgga catcgccgcc gatacgctgc actttgagca atttgctatc gaagacaaaa
    65641 ccggcgatgc atttaccctg gtgcttgccc gttccggaaa agagtttgtg gtgccggaag
    65701 agatgactat tttgcaggtt attgaaaata ataaagccgc gaaagtggaa tgtttatgtc
    65761 gtgaaggggt atgcggaacc tgcgaaacag caatactgga aggtgaagct gaccatcggg
    65821 atcaatattt tagcgatgaa gagcgtgcca gccagcaaag tatgttgatc tgttgttcgc
    65881 gtgcgaaggg taaacgcctg gtgttggatt tgtagtttgc cgaagccgga tgtggcgctg
    65941 agcgcgccca gtccggcttc ggaagatttt actgcggata ttcctgcaat aaattgtgta
    66001 atgcttccgc catcagctca ccacgccagc cggaaatcag ctccggcaaa ttgttctgcg
    66061 gtttcagttt ccagtgccag ttcagcagtt ggttgatttg ccgacgcgat gccagcaatt
    66121 cggcgctgat cttatgcgtt tcgctcacgt cagtaatcag cgacttaatc gctttaaacg
    66181 ctttacgata acccggcatg tccatcaggt taagcatcgg ctgcggtaag gcatcttccg
    66241 gcaatgtctg cgctttttcc accagcgcca gcagcgtttt accgtgaaag cggatttcgc
    66301 taccggataa acccaggctg tccagttcgc ctaaacttcc cggcatataa cgcgctaccg
    66361 accacaaatg ctcttcacgc acgacaaagt tcaccgccag atcgcgctct cgcgccttgc
    66421 gcagtcgcca gtcggctaac agttgcagac aggccagttg gcgtgtgcgt aattgccagg
    66481 cattggtgat atcacgccag gcatcttccg gcgcaacgac ttcctgacga cgcatttgca
    66541 tcaggcggca ttcatccagc gccgcaggta gccagccgga gtcctccgtt tctaccataa
    66601 gcttggcggt gatcggtaac agataccaga catccgccgc tgcgtattca cactgacgtt
    66661 cggtcagcgg tctggccagc cagtcggtgc gcgattcact cttgtccagc gtaacgccgg
    66721 aatactcttc caccatggaa gcgaaacccc atgacatcgg gcgtccgcag aaggcagcaa
    66781 ggatttgcgt gtcaatcaag ggttgtggta attcgccaaa gacattgagg aacacttcca
    66841 gatcttcact gcctgcatgg agaaattttg tgatggacgg atcgcgcagg atcgctttca
    66901 gcggtgacca gtcggtgatc ccgagtggat cgattagcgc cagatgctcg ccatcgaaaa
    66961 gttgaatcaa ccccagctgc gggtaataag tgcgcgtacg aacaaattca gtatccaggg
    67021 ctatcgccgg aaaggcacgg acggcttcac acaaagaagc cagcgcatcg tccgtggtaa
    67081 tcatttggta attcaaattg ttttctcttt agtgggcgtc aaaaaaaacg ccggattaac
    67141 cggcgtctga cgactgactt aacgctcagg ctttattgtc cactttgccg cgcgcttcgt
    67201 cacgtaattc tcgtcgcaaa atttttccga cgttagattt cggtaactca tcacgaaact
    67261 ccaccagctt cggtactttg tatcccgtga gctgacggcg gcaaaaagtc accagtgact
    67321 cttcggtaag cgatggatct tttttcacta cgaagatttt caccgcttca ccactggagc
    67381 cggaaggtac gccaacagcc gcgacttcct gtacgccagg atgctgcatg acaacatctt
    67441 caatctcgtt gggatagacg ttaaaaccgg aaaccagaat catgtctttt ttacgatcga
    67501 caatgcgcag gaatccttct tcatccatta ccgcgatgtc gccggtgtgt aaccagccat
    67561 ttttgatgat ttcatcggta gcatcgggac gctgccagta acccagcatc acctgcggtc
    67621 ctttgacaca aagctcaccc ggttgacctg gtggtacttc attatcatca tcatccacca
    67681 gtttggcttc cgtcgacggc accggcaaac cgatgctacc actatgataa tcaatatcat
    67741 atgggttaac gctgaccagc ggcgcacact cggtaaggcc atagccttcc agcagatact
    67801 gtccggtcag tttcacccaa cgctctgcca ccacttgctg cactggcatc ccaccgcctg
    67861 cggaaagatg cagactggag aaatccagct gctggaactc tttattgttc agcaacgcat
    67921 tgaacaaggt gttaacgcca gtgatagcgg taaacggata tttcgctaac tcttttacca
    67981 accctggaat atcacgcggg ttagtgataa gcaggttctg cccacccagt tcgataaaca
    68041 gcaggcagtt aatggtcagg gcaaaaatgt gatacagcgg cagtgccgtt actaccagtt
    68101 ctttacctgg atgcagcaac ggcccatagg tggcgttgac ctgttccaag ttcgccagca
    68161 tattgcggtg agtcagcatc gcgcctttcg ccacacctgt ggtgccgccg gtgtattgca
    68221 ggaaggctaa atcttccggc accagttcgg gtttgacgta ctgcatccgg tagccgttat
    68281 gcagtgcgct acgaaatgaa atggcatctg gcagatggta tttcggcacc aaacgcttga
    68341 tgtatttaac aacgaaattg actaccgtgc cttttgccgt agatagctga tcgcccatac
    68401 gggtcagaat tacgtgctga acggcggttt tatcaaccac tttttccagt gtgtgagcaa
    68461 agttagacac gataacaatc gccgatgcgc cgctatcgtt aagctgatgc tcaagctcac
    68521 gcggggtata caacgggtta acgtttacga cgatcatccc ggcacgcaaa atgccaaaca
    68581 gcgccaccgg atattgcaat aaattaggca tcatcaacgc aacgcgatcg cctttcttca
    68641 gccccaaccc ttgttgcaaa taagcggcaa acgcgcgact gcgttcttcc agcttgcaga
    68701 aggtcatcac ctcccccata ttcacaaacg caggctgatc ggcgtagcgc gcgaccgact
    68761 gctcaaacat atctaccaga gattgataac ggtcagggtt gatctccgtc ggaacgtccg
    68821 cgggataacg gttaagccaa accttcttca attcttcacc tctaaaatac gtgttcgtcg
    68881 tcatcgcaac cccaaatgat atacatgctg ttaacataat attaactcat cataccagct
    68941 tgataattac ccaacgaaaa ggttgcgaag cgcgtcacta tttattttta tctttaccgt
    69001 aagaatgcag aaacagcgga ccagccgctg tttctttttc tttaaaaaca agcaattatt
    69061 cagttacaac tgtttgtact ctcgcgggac cgggattata ccagccccat ccgccgtagc
    69121 catagggcca gccacgaccg ccataaaacc atggatcaat cggctgaggc ggcataatca
    69181 cctgctgggt taaatgccaa cgtttgtaac ccgttacttg catcaccata aatttatagg
    69241 gcgtattgcc gattttgccg tcaaccgcac cagtgattgg cccgactacc gtaaccagtt
    69301 gtccacggaa atccaccggg tccagaaaac cgttcacatc ggcataaatg cgaccgcgag
    69361 aaggttctcc cagcgtcggt ctggctccgc tgtccagcgg tacggtagca atttccaggc
    69421 gggttttccc ttgctggttt tgtaccgcaa ccactttgcc accaaagcgt gcctcctgac
    69481 caacgtacag ctgcggcgca ctcatcaccc gaactaaatc ttgttgcggc gtgggactgc
    69541 tgcctttaat ggcgtccggc acagtgacac aaccgctcag cattagcgca aacgtacctg
    69601 ccagtatgcc tttgataaca ttcttttgaa ccgccatgat gcgactcctt tttttctcag
    69661 ggcatactct taagattcat tctttgcccg gaagtttctt ccatgcgacg ttgttacgta
    69721 aataaaccgg ctcggcatgt tccaccgcca ccgttttacc ctcggcaaac atctgacaag
    69781 caatcggcag catatcttct gcagcgggga gtaacacttc gccatcgcgc aaaaccagcc
    69841 cgctctcttt accgagatcc ggccaggctt gccagcccgt ccctaccgtc acccattcgc
    69901 cggaaagctg ttgcattcgt tcatggacga tttcgggttt gagtacggct tcggtttctt
    69961 caccgtgcca gataccgttt tcatcacgct gatattcggc ccagtaaact tcgcccattc
    70021 gcgcgtcaat ggctgacagc acgcgtgttg cgccgttttt gcgccacgcc ccttgcgcca
    70081 tcgtcattag cgtggagacg ccaatcatcg gtaattccgc gccaagcgcc agcccttgcg
    70141 cgatgccaat accaatgcgc acgccggtaa agctaccggg gccgcgcccg taagccagag
    70201 cgttaatatc agtcagggaa gttccgctgg tggtcaggat atcctgcacc atcggtaaga
    70261 ttcgttgagt atgttcacga gggcaaagct caaaatgagc gttgacagta ccgtcgttcc
    70321 acagggcgac agagcaggcc tctgtcgcgg tatcgatagc cagaattcgc atgggtcttc
    70381 gtgcttagat caataaaaag gcgcgcatca taccatactc cgtaacaaat tacctggagg
    70441 atggtatcgc aaggaaacga accgcacggg caatgtcacg ggtgcgtggc gcgggcggca
    70501 gactggcgag aaacgtcgcg ccgtaaggac gcatcaccag ccgattgtca caaatcacca
    70561 gcacgccacg atcgtcggcg tcgcgaatca gtcgccctac cccctgtttg agagtaatga
    70621 cggcatctgg tagttgcact tcatcgaacg ggtcgccacc gcgcaaacga caatcttcca
    70681 tgcgcgcttt taacagtgga tcatccggcg aggtaaacgg caatttgtcg ataattacca
    70741 atgacaatgt atcgccacgc acgtccaccc cttcccagaa actactggtt gccacaagaa
    70801 gcgcattacc ggcactgaca aactgctgca acagttggcc tttactggtt tccccctgca
    70861 acaaaacggg aagcgtcatg gtagcgcgga actgctcggc cagatcgcgc atcatggcgt
    70921 gcgaggtaca aagcataaaa caacgaccgt tgttagcttc gatgatcggt cgcagcattg
    70981 ccgctaactg gcgagcagaa cctggctggt tggtttgcgg cagattgcgc ggcacacaga
    71041 gtaacgcctg gcggctgtaa tcaaacgggc ttggcaatag caacgactcc gcctgtgcta
    71101 tcccaagccg cgaggtgaaa tgatgcagat cgtcgttcac cgacagcgtt gctgaggtga
    71161 agatccagct accgggcttt tgcgccatta actctttgaa tttatccgcc acgctgagcg
    71221 gcgtgagagc cagagtaaaa tggcgcgaag tgcattcgta ccagtagctg tagcccggct
    71281 gattgatctc ttttagccgc ttcagccgtg tgcgatacaa cgtggcgcgc tcaaatgccg
    71341 catccagcaa ggcggaacgc cccagtgaca gtttcgccac gtcataacaa agttccaggg
    71401 tgtcatcgag cagtaaaaat gcccgctgaa tttgcggatt agctaacagc tcacgcaggt
    71461 taccgcgata acctggctca ccgagttgca gacgaaaatc ctgcgcactc tgggcaagac
    71521 gatcagcgca cttttgtaac tgctgggtgt cttttaattc ggtgcggtag gcgatggtga
    71581 tgtcttttgc caggtcaagc agttgtcgac tggagagtga ctgaccaaaa tactggctgg
    71641 caatgtccgg tagctggtgg gcttcgtcga agatcatgac gtccgcttcc gggatcaact
    71701 cgccaaatcc actctcttta accaccatat ccgctagaaa gagatgatgg tttaccacca
    71761 ccacatcggc gtccatcgct tttttacgtg ctttgaccac aaagcaatct ttatacatcg
    71821 ggcagtcgct gccaagacag ttgtcgttgg tgctggtgac cagcggccac gcctgtgaat
    71881 cttccgccac gctgacgcag gtgctgatat caccatcgac tgtttgatta gaccaggagc
    71941 gcagcaggat cacatcgctt aagatttgta ccagcagatc gccccccgcc agcgcctgct
    72001 gttcgagacg ttcaaggcag aggtagtttg agcgcccttt cagcagcgcc acgttgcccg
    72061 tgtatttcaa cgcctttgag actgttggca aatcgcggct gtagagctga tcctgcaacg
    72121 cttttgagcc ggtcgagata atgactttct ttttcgcccg cagcgcagga gccaggtaag
    72181 cgtaggtttt gcccgtaccg gttcctgctt ccaccaccag cggctggcct ttttctatcg
    72241 cctgggtgac ggctaccgcc atctgtcgct gtggttctcg cggcttaaag cctggtatcg
    72301 ctttcgccag ctgaccgtct ggtgcaaaat cgtccgtcac actaccccct gttgatttga
    72361 acagggatta tgtcaggatg agggcgcttt cgccagttga agtggtgacg gcgacctcac
    72421 attgtggcag tctttgcagc acgaaaatgg aagtataatg aggacaaaat gatgactatc
    72481 gttcgtatcg atgctgaagc ccgctggtct gatgtagtaa tccacaacaa cacgctctac
    72541 tacactggtg taccggaaaa cctcgacgcc gatgcctttg agcaaaccgc caacacgctg
    72601 gcacagattg acgccgtgct ggaaaaacag ggcagcaata aatcgagcat tctggatgcc
    72661 accattttcc tggccgataa aaacgacttc gcggcgatga ataaagcgtg ggatgcctgg
    72721 gttgtcgcgg gtcatgcgcc ggtgcgctgc acggtacaag cgggtttgat gaacccgaag
    72781 tataaagttg aaattaagat tgtggctgcg gtgtaagctt tatcgaagca aaataagtca
    72841 gacgataatt tatcgataat actggtcggt tttacataaa tcgaccagag aa
//
LOCUS       DAZKHC020000010        70967 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZKHC020000010 DAZKHC020000000
VERSION     DAZKHC020000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 70967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.010"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1111)
                     /gene="hemW"
                     /locus_tag="ACHOSM_RS04105"
                     /old_locus_tag="pgaptmp_000821"
     CDS             complement(<1..1111)
                     /gene="hemW"
                     /locus_tag="ACHOSM_RS04105"
                     /old_locus_tag="pgaptmp_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGU4557400.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVTYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNS"
     gene            complement(1104..1697)
                     /gene="rdgB"
                     /locus_tag="ACHOSM_RS04110"
                     /old_locus_tag="pgaptmp_000822"
     CDS             complement(1104..1697)
                     /gene="rdgB"
                     /locus_tag="ACHOSM_RS04110"
                     /old_locus_tag="pgaptmp_000822"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HGU4557401.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(1705..1995)
                     /gene="yggU"
                     /locus_tag="ACHOSM_RS04115"
                     /old_locus_tag="pgaptmp_000823"
     CDS             complement(1705..1995)
                     /gene="yggU"
                     /locus_tag="ACHOSM_RS04115"
                     /old_locus_tag="pgaptmp_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HGU4557402.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(1992..2558)
                     /gene="yggT"
                     /locus_tag="ACHOSM_RS04120"
                     /old_locus_tag="pgaptmp_000824"
     CDS             complement(1992..2558)
                     /gene="yggT"
                     /locus_tag="ACHOSM_RS04120"
                     /old_locus_tag="pgaptmp_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HGU4557403.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWTHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(2576..3280)
                     /gene="yggS"
                     /locus_tag="ACHOSM_RS04125"
                     /old_locus_tag="pgaptmp_000825"
     CDS             complement(2576..3280)
                     /gene="yggS"
                     /locus_tag="ACHOSM_RS04125"
                     /old_locus_tag="pgaptmp_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HGU4557404.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            3298..4278
                     /gene="yggR"
                     /locus_tag="ACHOSM_RS04130"
                     /old_locus_tag="pgaptmp_000826"
     CDS             3298..4278
                     /gene="yggR"
                     /locus_tag="ACHOSM_RS04130"
                     /old_locus_tag="pgaptmp_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HGU4557405.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(4454..4870)
                     /gene="ruvX"
                     /locus_tag="ACHOSM_RS04135"
                     /old_locus_tag="pgaptmp_000827"
     CDS             complement(4454..4870)
                     /gene="ruvX"
                     /locus_tag="ACHOSM_RS04135"
                     /old_locus_tag="pgaptmp_000827"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGU4557406.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVVILESYFEQGY"
     gene            complement(4870..5433)
                     /gene="yqgE"
                     /locus_tag="ACHOSM_RS04140"
                     /old_locus_tag="pgaptmp_000828"
     CDS             complement(4870..5433)
                     /gene="yqgE"
                     /locus_tag="ACHOSM_RS04140"
                     /old_locus_tag="pgaptmp_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HGU4557407.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(5542..6492)
                     /gene="gshB"
                     /locus_tag="ACHOSM_RS04145"
                     /old_locus_tag="pgaptmp_000829"
     CDS             complement(5542..6492)
                     /gene="gshB"
                     /locus_tag="ACHOSM_RS04145"
                     /old_locus_tag="pgaptmp_000829"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HGU4557408.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(6505..7236)
                     /gene="rsmE"
                     /locus_tag="ACHOSM_RS04150"
                     /old_locus_tag="pgaptmp_000830"
     CDS             complement(6505..7236)
                     /gene="rsmE"
                     /locus_tag="ACHOSM_RS04150"
                     /old_locus_tag="pgaptmp_000830"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGU4557409.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSSSKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(7316..8023)
                     /gene="endA"
                     /locus_tag="ACHOSM_RS04155"
                     /old_locus_tag="pgaptmp_000831"
     CDS             complement(7316..8023)
                     /gene="endA"
                     /locus_tag="ACHOSM_RS04155"
                     /old_locus_tag="pgaptmp_000831"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HGU4557410.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(8118..8615)
                     /gene="yggI"
                     /locus_tag="ACHOSM_RS04160"
                     /old_locus_tag="pgaptmp_000832"
     CDS             complement(8118..8615)
                     /gene="yggI"
                     /locus_tag="ACHOSM_RS04160"
                     /old_locus_tag="pgaptmp_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HGU4557411.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLAWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(8692..10086)
                     /gene="galP"
                     /locus_tag="ACHOSM_RS04165"
                     /old_locus_tag="pgaptmp_000833"
     CDS             complement(8692..10086)
                     /gene="galP"
                     /locus_tag="ACHOSM_RS04165"
                     /old_locus_tag="pgaptmp_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HGU4557412.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(10522..11676)
                     /gene="metK"
                     /locus_tag="ACHOSM_RS04170"
                     /old_locus_tag="pgaptmp_000834"
     CDS             complement(10522..11676)
                     /gene="metK"
                     /locus_tag="ACHOSM_RS04170"
                     /old_locus_tag="pgaptmp_000834"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGU4557413.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            11732..11983
                     /gene="yqgD"
                     /locus_tag="ACHOSM_RS04175"
                     /old_locus_tag="pgaptmp_000835"
     CDS             11732..11983
                     /gene="yqgD"
                     /locus_tag="ACHOSM_RS04175"
                     /old_locus_tag="pgaptmp_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HGU4557414.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            12094..12237
                     /gene="yqgG"
                     /locus_tag="ACHOSM_RS04180"
                     /old_locus_tag="pgaptmp_000836"
     CDS             12094..12237
                     /gene="yqgG"
                     /locus_tag="ACHOSM_RS04180"
                     /old_locus_tag="pgaptmp_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HGU4557415.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            12331..12462
                     /gene="yqgB"
                     /locus_tag="ACHOSM_RS04185"
                     /old_locus_tag="pgaptmp_000837"
     CDS             12331..12462
                     /gene="yqgB"
                     /locus_tag="ACHOSM_RS04185"
                     /old_locus_tag="pgaptmp_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HGU4557416.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            12471..14447
                     /gene="speA"
                     /locus_tag="ACHOSM_RS04190"
                     /old_locus_tag="pgaptmp_000838"
     CDS             12471..14447
                     /gene="speA"
                     /locus_tag="ACHOSM_RS04190"
                     /old_locus_tag="pgaptmp_000838"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HGU4557417.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            14585..15505
                     /gene="speB"
                     /locus_tag="ACHOSM_RS04195"
                     /old_locus_tag="pgaptmp_000839"
     CDS             14585..15505
                     /gene="speB"
                     /locus_tag="ACHOSM_RS04195"
                     /old_locus_tag="pgaptmp_000839"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HGU4557418.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(15711..16469)
                     /gene="loiP"
                     /locus_tag="ACHOSM_RS04200"
                     /old_locus_tag="pgaptmp_000840"
                     /gene_synonym="yggG"
     CDS             complement(15711..16469)
                     /gene="loiP"
                     /locus_tag="ACHOSM_RS04200"
                     /old_locus_tag="pgaptmp_000840"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HGU4557419.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKALSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            16747..18738
                     /gene="tkt"
                     /locus_tag="ACHOSM_RS04205"
                     /old_locus_tag="pgaptmp_000841"
     CDS             16747..18738
                     /gene="tkt"
                     /locus_tag="ACHOSM_RS04205"
                     /old_locus_tag="pgaptmp_000841"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU4557420.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIVRGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            19052..19495
                     /gene="cmtB"
                     /locus_tag="ACHOSM_RS04210"
                     /old_locus_tag="pgaptmp_000842"
     CDS             19052..19495
                     /gene="cmtB"
                     /locus_tag="ACHOSM_RS04210"
                     /old_locus_tag="pgaptmp_000842"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HGU4557421.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            19523..20911
                     /gene="cmtA"
                     /locus_tag="ACHOSM_RS04215"
                     /old_locus_tag="pgaptmp_000843"
     CDS             19523..20911
                     /gene="cmtA"
                     /locus_tag="ACHOSM_RS04215"
                     /old_locus_tag="pgaptmp_000843"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HGU4557422.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            20926..22203
                     /gene="yggP"
                     /locus_tag="ACHOSM_RS04220"
                     /old_locus_tag="pgaptmp_000844"
     CDS             20926..22203
                     /gene="yggP"
                     /locus_tag="ACHOSM_RS04220"
                     /old_locus_tag="pgaptmp_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU4557423.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPMFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            22200..23165
                     /gene="glpX"
                     /locus_tag="ACHOSM_RS04225"
                     /old_locus_tag="pgaptmp_000845"
     CDS             22200..23165
                     /gene="glpX"
                     /locus_tag="ACHOSM_RS04225"
                     /old_locus_tag="pgaptmp_000845"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU4557424.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGVIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQESKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            23187..23696
                     /gene="fumE"
                     /locus_tag="ACHOSM_RS04230"
                     /old_locus_tag="pgaptmp_000846"
     CDS             23187..23696
                     /gene="fumE"
                     /locus_tag="ACHOSM_RS04230"
                     /old_locus_tag="pgaptmp_000846"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HGU4557425.1"
                     /translation="MAKLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPLLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            23693..24406
                     /locus_tag="ACHOSM_RS04235"
                     /old_locus_tag="pgaptmp_000847"
     CDS             23693..24406
                     /locus_tag="ACHOSM_RS04235"
                     /old_locus_tag="pgaptmp_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HGU4557426.1"
                     /translation="MKIELAVNGLKIQAQYQNEEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(24378..25055)
                     /locus_tag="ACHOSM_RS04240"
                     /old_locus_tag="pgaptmp_000848"
     CDS             complement(24378..25055)
                     /locus_tag="ACHOSM_RS04240"
                     /old_locus_tag="pgaptmp_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4557427.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(25049..25726)
                     /locus_tag="ACHOSM_RS04245"
                     /old_locus_tag="pgaptmp_000849"
     CDS             complement(25049..25726)
                     /locus_tag="ACHOSM_RS04245"
                     /old_locus_tag="pgaptmp_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4557428.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(25714..26421)
                     /locus_tag="ACHOSM_RS04250"
                     /old_locus_tag="pgaptmp_000850"
     CDS             complement(25714..26421)
                     /locus_tag="ACHOSM_RS04250"
                     /old_locus_tag="pgaptmp_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGU4557429.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTYNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(26422..27000)
                     /locus_tag="ACHOSM_RS04255"
                     /old_locus_tag="pgaptmp_000851"
     CDS             complement(26422..27000)
                     /locus_tag="ACHOSM_RS04255"
                     /old_locus_tag="pgaptmp_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGU4557430.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVKSVAITVIGANL
                     VDKILTAVIVWLLLSQLPIRTTRHFPAMAAVR"
     gene            complement(27023..27453)
                     /locus_tag="ACHOSM_RS04260"
                     /old_locus_tag="pgaptmp_000852"
                     /pseudo
     CDS             complement(27023..27453)
                     /locus_tag="ACHOSM_RS04260"
                     /old_locus_tag="pgaptmp_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
     gene            27825..28844
                     /gene="epd"
                     /locus_tag="ACHOSM_RS04265"
                     /old_locus_tag="pgaptmp_000853"
     CDS             27825..28844
                     /gene="epd"
                     /locus_tag="ACHOSM_RS04265"
                     /old_locus_tag="pgaptmp_000853"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HGU4557431.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQEQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            28894..30057
                     /gene="pgk"
                     /locus_tag="ACHOSM_RS04270"
                     /old_locus_tag="pgaptmp_000854"
     CDS             28894..30057
                     /gene="pgk"
                     /locus_tag="ACHOSM_RS04270"
                     /old_locus_tag="pgaptmp_000854"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGU4557432.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            30272..31351
                     /gene="fbaA"
                     /locus_tag="ACHOSM_RS04275"
                     /old_locus_tag="pgaptmp_000855"
     CDS             30272..31351
                     /gene="fbaA"
                     /locus_tag="ACHOSM_RS04275"
                     /old_locus_tag="pgaptmp_000855"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGU4557433.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(31415..31513)
                     /locus_tag="ACHOSM_RS04280"
                     /old_locus_tag="pgaptmp_000856"
                     /pseudo
     CDS             complement(31415..31513)
                     /locus_tag="ACHOSM_RS04280"
                     /old_locus_tag="pgaptmp_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            31709..32569
                     /gene="mscS"
                     /locus_tag="ACHOSM_RS04285"
                     /old_locus_tag="pgaptmp_000857"
     CDS             31709..32569
                     /gene="mscS"
                     /locus_tag="ACHOSM_RS04285"
                     /old_locus_tag="pgaptmp_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HGU4557434.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            32708..33343
                     /gene="argO"
                     /locus_tag="ACHOSM_RS04290"
                     /old_locus_tag="pgaptmp_000858"
     CDS             32708..33343
                     /gene="argO"
                     /locus_tag="ACHOSM_RS04290"
                     /old_locus_tag="pgaptmp_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HGU4557435.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            33436..34176
                     /gene="yggE"
                     /locus_tag="ACHOSM_RS04295"
                     /old_locus_tag="pgaptmp_000859"
     CDS             33436..34176
                     /gene="yggE"
                     /locus_tag="ACHOSM_RS04295"
                     /old_locus_tag="pgaptmp_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HGU4557436.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPIVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            34343..35239
                     /gene="ygfI"
                     /locus_tag="ACHOSM_RS04300"
                     /old_locus_tag="pgaptmp_000860"
     CDS             34343..35239
                     /gene="ygfI"
                     /locus_tag="ACHOSM_RS04300"
                     /old_locus_tag="pgaptmp_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4557437.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIVEDRDGTNH"
     gene            complement(35236..36714)
                     /gene="scpC"
                     /locus_tag="ACHOSM_RS04305"
                     /old_locus_tag="pgaptmp_000861"
     CDS             complement(35236..36714)
                     /gene="scpC"
                     /locus_tag="ACHOSM_RS04305"
                     /old_locus_tag="pgaptmp_000861"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HGU4557438.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(36738..37523)
                     /gene="scpB"
                     /locus_tag="ACHOSM_RS04310"
                     /old_locus_tag="pgaptmp_000862"
     CDS             complement(36738..37523)
                     /gene="scpB"
                     /locus_tag="ACHOSM_RS04310"
                     /old_locus_tag="pgaptmp_000862"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HGU4557439.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(37534..38529)
                     /gene="meaB"
                     /locus_tag="ACHOSM_RS04315"
                     /old_locus_tag="pgaptmp_000863"
     CDS             complement(37534..38529)
                     /gene="meaB"
                     /locus_tag="ACHOSM_RS04315"
                     /old_locus_tag="pgaptmp_000863"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HGU4557440.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGPPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(38522..40666)
                     /gene="scpA"
                     /locus_tag="ACHOSM_RS04320"
                     /old_locus_tag="pgaptmp_000864"
     CDS             complement(38522..40666)
                     /gene="scpA"
                     /locus_tag="ACHOSM_RS04320"
                     /old_locus_tag="pgaptmp_000864"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HGU4557441.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQLWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKEGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAQEQGVTTDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLEHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(40870..41763)
                     /gene="argP"
                     /locus_tag="ACHOSM_RS04325"
                     /old_locus_tag="pgaptmp_000865"
     CDS             complement(40870..41763)
                     /gene="argP"
                     /locus_tag="ACHOSM_RS04325"
                     /old_locus_tag="pgaptmp_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HGU4557442.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            42146..42805
                     /gene="rpiA"
                     /locus_tag="ACHOSM_RS04330"
                     /old_locus_tag="pgaptmp_000866"
     CDS             42146..42805
                     /gene="rpiA"
                     /locus_tag="ACHOSM_RS04330"
                     /old_locus_tag="pgaptmp_000866"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGU4557443.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            43061..44293
                     /gene="serA"
                     /locus_tag="ACHOSM_RS04335"
                     /old_locus_tag="pgaptmp_000867"
     CDS             43061..44293
                     /gene="serA"
                     /locus_tag="ACHOSM_RS04335"
                     /old_locus_tag="pgaptmp_000867"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HGU4557444.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            44522..44581
                     /gene="ibsC"
                     /locus_tag="ACHOSM_RS04340"
                     /old_locus_tag="pgaptmp_000868"
     CDS             44522..44581
                     /gene="ibsC"
                     /locus_tag="ACHOSM_RS04340"
                     /old_locus_tag="pgaptmp_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HGU4557445.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            44914..44973
                     /locus_tag="ACHOSM_RS04345"
                     /old_locus_tag="pgaptmp_000869"
     CDS             44914..44973
                     /locus_tag="ACHOSM_RS04345"
                     /old_locus_tag="pgaptmp_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4557446.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(45074..45676)
                     /gene="fau"
                     /locus_tag="ACHOSM_RS04350"
                     /old_locus_tag="pgaptmp_000870"
     CDS             complement(45074..45676)
                     /gene="fau"
                     /locus_tag="ACHOSM_RS04350"
                     /old_locus_tag="pgaptmp_000870"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGU4557447.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(45697..45880)
                     /gene="ssrS"
                     /locus_tag="ACHOSM_RS04355"
                     /old_locus_tag="pgaptmp_000871"
     ncRNA           complement(45697..45880)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHOSM_RS04355"
                     /old_locus_tag="pgaptmp_000871"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(45922..46251)
                     /gene="zapA"
                     /locus_tag="ACHOSM_RS04360"
                     /old_locus_tag="pgaptmp_000872"
     CDS             complement(45922..46251)
                     /gene="zapA"
                     /locus_tag="ACHOSM_RS04360"
                     /old_locus_tag="pgaptmp_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU4557448.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            46420..46998
                     /gene="ygfB"
                     /locus_tag="ACHOSM_RS04365"
                     /old_locus_tag="pgaptmp_000873"
     CDS             46420..46998
                     /gene="ygfB"
                     /locus_tag="ACHOSM_RS04365"
                     /old_locus_tag="pgaptmp_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HGU4557449.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            47024..48349
                     /gene="pepP"
                     /locus_tag="ACHOSM_RS04370"
                     /old_locus_tag="pgaptmp_000874"
     CDS             47024..48349
                     /gene="pepP"
                     /locus_tag="ACHOSM_RS04370"
                     /old_locus_tag="pgaptmp_000874"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HGU4557450.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEISAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLCLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            48346..49524
                     /gene="ubiH"
                     /locus_tag="ACHOSM_RS04375"
                     /old_locus_tag="pgaptmp_000875"
                     /gene_synonym="visB"
     CDS             48346..49524
                     /gene="ubiH"
                     /locus_tag="ACHOSM_RS04375"
                     /old_locus_tag="pgaptmp_000875"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HGU4557451.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANFATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            49547..50749
                     /gene="ubiI"
                     /locus_tag="ACHOSM_RS04380"
                     /old_locus_tag="pgaptmp_000876"
                     /gene_synonym="visC"
     CDS             49547..50749
                     /gene="ubiI"
                     /locus_tag="ACHOSM_RS04380"
                     /old_locus_tag="pgaptmp_000876"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HGU4557452.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLVADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(50823..50906)
                     /locus_tag="ACHOSM_RS04385"
                     /old_locus_tag="pgaptmp_000877"
     CDS             complement(50823..50906)
                     /locus_tag="ACHOSM_RS04385"
                     /old_locus_tag="pgaptmp_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557453.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            51196..52290
                     /gene="gcvT"
                     /locus_tag="ACHOSM_RS04390"
                     /old_locus_tag="pgaptmp_000878"
     CDS             51196..52290
                     /gene="gcvT"
                     /locus_tag="ACHOSM_RS04390"
                     /old_locus_tag="pgaptmp_000878"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HGU4557454.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            52314..52703
                     /gene="gcvH"
                     /locus_tag="ACHOSM_RS04395"
                     /old_locus_tag="pgaptmp_000879"
     CDS             52314..52703
                     /gene="gcvH"
                     /locus_tag="ACHOSM_RS04395"
                     /old_locus_tag="pgaptmp_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HGU4557455.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            52822..55695
                     /gene="gcvP"
                     /locus_tag="ACHOSM_RS04400"
                     /old_locus_tag="pgaptmp_000880"
     CDS             52822..55695
                     /gene="gcvP"
                     /locus_tag="ACHOSM_RS04400"
                     /old_locus_tag="pgaptmp_000880"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HGU4557456.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIYDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            55961..56704
                     /gene="ygfF"
                     /locus_tag="ACHOSM_RS04405"
                     /old_locus_tag="pgaptmp_000881"
     CDS             55961..56704
                     /gene="ygfF"
                     /locus_tag="ACHOSM_RS04405"
                     /old_locus_tag="pgaptmp_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4557457.1"
                     /translation="MAIALVTGGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(56761..58194)
                     /gene="bglA"
                     /locus_tag="ACHOSM_RS04410"
                     /old_locus_tag="pgaptmp_000882"
     CDS             complement(56761..58194)
                     /gene="bglA"
                     /locus_tag="ACHOSM_RS04410"
                     /old_locus_tag="pgaptmp_000882"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HGU4557458.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDLFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            58239..58550
                     /gene="yqfB"
                     /locus_tag="ACHOSM_RS04415"
                     /old_locus_tag="pgaptmp_000883"
     CDS             58239..58550
                     /gene="yqfB"
                     /locus_tag="ACHOSM_RS04415"
                     /old_locus_tag="pgaptmp_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HGU4557459.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            58714..59373
                     /gene="yqfA"
                     /locus_tag="ACHOSM_RS04420"
                     /old_locus_tag="pgaptmp_000884"
     CDS             58714..59373
                     /gene="yqfA"
                     /locus_tag="ACHOSM_RS04420"
                     /old_locus_tag="pgaptmp_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGU4557460.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(59569..60549)
                     /gene="ygfZ"
                     /locus_tag="ACHOSM_RS04425"
                     /old_locus_tag="pgaptmp_000885"
     CDS             complement(59569..60549)
                     /gene="ygfZ"
                     /locus_tag="ACHOSM_RS04425"
                     /old_locus_tag="pgaptmp_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HGU4557461.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            60792..61058
                     /gene="sdhE"
                     /locus_tag="ACHOSM_RS04430"
                     /old_locus_tag="pgaptmp_000886"
     CDS             60792..61058
                     /gene="sdhE"
                     /locus_tag="ACHOSM_RS04430"
                     /old_locus_tag="pgaptmp_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HGU4557462.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            61039..61446
                     /gene="ygfX"
                     /locus_tag="ACHOSM_RS04435"
                     /old_locus_tag="pgaptmp_000887"
     CDS             61039..61446
                     /gene="ygfX"
                     /locus_tag="ACHOSM_RS04435"
                     /old_locus_tag="pgaptmp_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HGU4557463.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(61486..62007)
                     /gene="fldB"
                     /locus_tag="ACHOSM_RS04440"
                     /old_locus_tag="pgaptmp_000888"
     CDS             complement(61486..62007)
                     /gene="fldB"
                     /locus_tag="ACHOSM_RS04440"
                     /old_locus_tag="pgaptmp_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HGU4557464.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            62119..63015
                     /gene="xerD"
                     /locus_tag="ACHOSM_RS04445"
                     /old_locus_tag="pgaptmp_000889"
     CDS             62119..63015
                     /gene="xerD"
                     /locus_tag="ACHOSM_RS04445"
                     /old_locus_tag="pgaptmp_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGU4557465.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            63039..63749
                     /gene="dsbC"
                     /locus_tag="ACHOSM_RS04450"
                     /old_locus_tag="pgaptmp_000890"
     CDS             63039..63749
                     /gene="dsbC"
                     /locus_tag="ACHOSM_RS04450"
                     /old_locus_tag="pgaptmp_000890"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HGU4557466.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGAAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQIADYNALGITVRYLAFPRQGLESQAEQQMKAIW
                     CAKDKNKAFDDVMAGKAATPASCDIDIADHYALGVQLGVSGTPAIVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            63755..65488
                     /gene="recJ"
                     /locus_tag="ACHOSM_RS04455"
                     /old_locus_tag="pgaptmp_000891"
     CDS             63755..65488
                     /gene="recJ"
                     /locus_tag="ACHOSM_RS04455"
                     /old_locus_tag="pgaptmp_000891"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGU4557467.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVSQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            65579..66677
                     /gene="prfB"
                     /locus_tag="ACHOSM_RS04460"
                     /old_locus_tag="pgaptmp_000892"
     CDS             join(65579..65653,65655..66677)
                     /gene="prfB"
                     /locus_tag="ACHOSM_RS04460"
                     /old_locus_tag="pgaptmp_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGU4557468.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            66687..68204
                     /gene="lysS"
                     /locus_tag="ACHOSM_RS04465"
                     /old_locus_tag="pgaptmp_000893"
     CDS             66687..68204
                     /gene="lysS"
                     /locus_tag="ACHOSM_RS04465"
                     /old_locus_tag="pgaptmp_000893"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU4557469.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(68247..68795)
                     /gene="idi"
                     /locus_tag="ACHOSM_RS04470"
                     /old_locus_tag="pgaptmp_000894"
     CDS             complement(68247..68795)
                     /gene="idi"
                     /locus_tag="ACHOSM_RS04470"
                     /old_locus_tag="pgaptmp_000894"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGU4557470.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESCEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            68850..68921
                     /locus_tag="ACHOSM_RS04475"
                     /old_locus_tag="pgaptmp_000895"
     CDS             68850..68921
                     /locus_tag="ACHOSM_RS04475"
                     /old_locus_tag="pgaptmp_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557471.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(68918..69043)
                     /gene="yqfG"
                     /locus_tag="ACHOSM_RS04480"
                     /old_locus_tag="pgaptmp_000896"
     CDS             complement(68918..69043)
                     /gene="yqfG"
                     /locus_tag="ACHOSM_RS04480"
                     /old_locus_tag="pgaptmp_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HGU4557472.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(69045..70493)
                     /gene="uacT"
                     /locus_tag="ACHOSM_RS04485"
                     /old_locus_tag="pgaptmp_000897"
     CDS             complement(69045..70493)
                     /gene="uacT"
                     /locus_tag="ACHOSM_RS04485"
                     /old_locus_tag="pgaptmp_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HGU4557473.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVVMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        17044 a        17869 c        18998 g        17056 t
ORIGIN      
        1 gtgagtttaa gaataattta ccatgttccg ttatctgcca gtaatccgca cattcggtga
       61 gataaccctg ggcaatcgcc tcgtctaact gtgggcgaat cacatcttcg caaagcccgg
      121 tatacgcaat aaactccacg cgcggcgcgg cctccagcag acggaagcga ttcataaaga
      181 actcaaacgg cttatctgcg gcttcgacat cacgctggct ttccagatac cttccttgca
      241 taaaaccacg aggatgacgc gttttagtgg tacgcagaat gcgcccatcc gggaaggtga
      301 ctttgccgtg cgcgccgcag ccaataccga tgtagtcacc aaatcgccag tagttgagat
      361 tgtgctggca ctgataaccg ggtttggcgt aagcggaagt ttcatattgc tgataacccg
      421 ctgcggttaa taactgatgc ccctgttcga aaatatccca cagcgcgtca tcgtccggca
      481 gcaccggtgg tcgcgaacca aacagcgtat tgggttcgat ggtcagttga taccaggaaa
      541 gatgcggcgg attcagttca atggcctggc gtagatcgcc aagcgcctct tccagtgatt
      601 gatccggtag tccatgcatc aaatcaaggt taaagctacg taaccctaaa ccgctcgcca
      661 gctttgccgc tcgtttcgct tcttgcgggc catgaatgcg cccaagtcgt ttcagctttt
      721 cttcgctaaa actctgcaca ccaatagaga tgcggttcac accagcacgc tgataatcga
      781 caaagcgatc ggcttctacc gtaccagggt tcgcttccat agtaatttct gcatccgctg
      841 ccagcggcaa acgcgcacgc acgccgtcca gcagcgtttg catcgccggg ccggaaagca
      901 ggctcggcgt accaccgcca ataaagattg tctttacttc acggccctga gcgtaagtca
      961 catcgttgtc cagatcgcaa agcagatgct gaacgtaatc gtcgtgcggc acttctcctt
     1021 tcaacgcgtg agagttgaaa tcgcagtacg ggcatttctg cacgcaccac gggatgtgaa
     1081 tgtagagact cagcggcggt aatttaacca ttacgtaaag cgtccaacag cagtttcaat
     1141 gcctgaccac ggtgggaaat ggcgctcttt tcttcgcggg tcagttcggc agcggttttc
     1201 ccttcggaag gtacgaagaa gattggatca taaccaaagc caccagtgcc cgctggttca
     1261 cgagtaatca cgcccggcca gctaccgtgg cacaccagcg gagtcggatc ttccgcgtga
     1321 cgcagatata ccagcacgca gtggaaacgc gcctgacgtt ggtcgtccgg tacgtctttc
     1381 agtgtttcca gtagtttttg cagattcttt agatcggtcg cgtcttcacc ggaataacgc
     1441 gcggagtaaa tgcccggcgc gccgccaagc acatctaccg ccagaccaga gtcgtcggca
     1501 attgccggta aaccggtcac ttttgccgca tggcgcgctt tcagaatcgc gttttcgata
     1561 aaggtcaggc cagtttcttc agcggaatct acgccgaggt ctgtttgggc cacgatatca
     1621 agaccgaagt cgctaagcag cgacgccagc tcacgcactt taccggcatt gccggttgcg
     1681 aggacaactt tttgcatagg atacctaatt aattaacgcc gcgatttctg gcgggatttg
     1741 ttgcggatta atgattttaa tttgtttgtg gcggccaagt tcgccttttt caatcaccac
     1801 ctggcttttg gcaacccgga attgcttacc gagaaacttc accagatgac tgttggcctg
     1861 gccgtcaacc ggcggcgcgg taatggcgac tttaacttcg tcgccatgta aaccgacaat
     1921 agaatcacgg ctggctttcg gctgaatata gagccgtaaa accagaccgt catcattaac
     1981 tgttacggca ctcataacgc catccacagc cccggcagca gcatgtttcc ggttgcctgt
     2041 aatacttctg cgacacccat attgatgaca tacagcagca gaacgaggat catcggcgag
     2101 aaatcaatcc cacccattgc cggtagcagg cggcgaatcg ggcgcagcag cggatcggcc
     2161 agctgaatca gcacgtattc aatcggacta cgaccctggc ttacccagct cataatcgcc
     2221 atcaccagca ggacccagaa aatcagcagg ccgatggttt tcagcagaat cagtaaaccg
     2281 gcaatccaga tgattggcag gaaggtcacc actttaaaca gcacgatggc tttgataaaa
     2341 ctgagaatgt acgcaaccag cagcgaggcg ctatcaatgg gacccattgc cggaataacg
     2401 cggcgcagtg gcccgataat tggctgtgtt accttcacta caaactgtga gaaggggttg
     2461 taaaaatcac aatgagtcca ctgcatccag atgcgtaata acagcaccat ggtatacagc
     2521 tcaatgaccg ttgaaagcag gaaagtcaac gtattcatgg cgttccttta attccttatt
     2581 ttttagagta atcacgcgca ccaaaaattg cagtgccgat acgaaccatc gtgctacctg
     2641 ccgcaatggc ggcttccata tcgtccgaca ttcccagaga gagcgtgtcg atatgcgggt
     2701 agcgcgtttt cagaccggca aatgctacag ccatttggcg tgcaacttca aactgcctta
     2761 catattctga ctcaggcgca gggattgcca tcagcccgcg cagacgtaaa cgcggtagtt
     2821 cagcgaccgc agctgccagc tcgtccagtt ctgccagttg aatcccggac ttactgtttt
     2881 cgtcactaat attaatttga atcagtacgt taagaggggg aagttctgcc gggcgctggt
     2941 cgttgaggcg agtagcgatg cgcaaacggt cgatggtatg acaccagtcg aaatgctctg
     3001 ccaccaggcg gcttttatta gactgcaacg ggccaataaa atgccattct aatcctgtta
     3061 cgcccagttc ctgaaagtgg cgaattttat ctaccccttc ctgaacgtag ttttcaccaa
     3121 attgacgctg cccggcatca atggcttctg cgatggcgct cgcaggtttt gttttactga
     3181 ctgcaagcag cgtaatttct tctggagaac ggccgcaacg cgttgcagcc gctgagattt
     3241 tgtcccggac ctgtgccagg ttatgcgcaa tatcgttcat tttccgagga tcttagtatg
     3301 aatatggaag aaattgtggc ccttagtgta aagcataacg tctcggatct acacctgtgc
     3361 agcgcctggc ccgcacgatg gcgcattcgc gggctaatgg aagctgcgcc gtttgatgcg
     3421 ccggacgtcg aagagctact gcgggagtgg ctggatgacg atcagcgggc aatattgctg
     3481 gaaaatggcc agctggattt tgccgtgtcg ctggcggaaa accagcgatt gcgtggcagc
     3541 gcattcgcgc aacggcatgg catttcgctg gcgttacggc tgttaccttc gcactgcccg
     3601 cagctcgaac agcttggcgc accaacggta ttgccggaat tactcaagag cgagaatggc
     3661 ctgattctgg tgacgggggc gacggggagc ggcaaatcta ccacgctggc ggcgatggtt
     3721 ggctatctca atcaacatgc cgatgcgcat attctgacgc tggaagatcc tgttgaatat
     3781 atctatgcca gccagcgatg tttgatccag cagcgggaaa ttggtttgca ctgtatgacg
     3841 ttcgcatcag gattgcgggc cgcattgcgg gaagatcctg atgtgatttt gctcggagag
     3901 ctgcgtgaca gcgagacaat ccgtctggca ctgacggcgg cagaaaccgg gcatttggtg
     3961 ctggcaacat tacatacgcg tggtgccgcg caggcagttg agcgactggt ggattcattt
     4021 ccggcgcagg aaaaagatcc cgtacgtaat caactggcag gtagtttacg ggccgtgttg
     4081 tcacaaaagc tggaagtgga taaacaggaa ggacgcgtgg cgctgtttga attattgatt
     4141 aacactcccg cggtggggaa tttgattcgc gaagggaaaa cccaccagtt accgcatgtt
     4201 attcaaaccg ggcagcaggt ggggatgata acgtttcagc agagttatca gcagcgggtg
     4261 ggggaagggc gtttgtgacg ttgccatgcc cgatgcgcaa gtttataggg cctataaagg
     4321 caataaatgt ctgatgcgac gctaacgcgt cttatcagac ctacagccgc tgccagatgt
     4381 gtgctggata aggcgttcac gccgcatccg gcaagatggc ggtaacgact atccgccagg
     4441 cttttagatc gccttaatat ccctgctcga aatagctttc gagaataaca accgcagagg
     4501 cagagtcgac tttgcctttg ttgagcgccc gatagccgcc ctgttcaaac agaccggaac
     4561 gggcttccac ggtgctaaga cgttcgtcat ggagctttac ttcaacaccg aaacgaccat
     4621 gaatacggtt ggcaaattta cgcgctctgg cagtcaatgg ttgctcggtg ccgtccatat
     4681 tcagcggcaa accgacgatg atttcgtccg gctgccactc tttcagtaaa cgttcgatga
     4741 ggttccagtc cggcgtaccg tcctgtgctt taattgcagg caaagggcga gcagtgccgg
     4801 taatgcgttg gccgaccgct acgccaatgc ttttggtgcc gaagtcgaag gcgagtaagg
     4861 ttccactcat caggcgtgcc ctgccacacc aggcatggtg agaatatcca caccaatcag
     4921 ttttgccgcc tcgcgccagc ggtcggcaat cggcgttttg aacagaatat tcagatccgc
     4981 cggggccgtt agccacgcgt tatcgagaat ttcttgttcc agttgacctt tttcccagga
     5041 ggcataaccc agagccacca atacgtcaga cggttgttta tcggtgccga gcgtttccag
     5101 cacatcgcgg gaagtggtca tcactgtgtt gtctgaaatg cgaatgctgg aagcaaaatt
     5161 ggagggcgga gtatgcaaaa taaacccgcg atcttcagcc agcggaccgc cgagcataac
     5221 cggtttatcc agacggattg attcatcacg cggctccggc gtgatcttca gcttttccag
     5281 aatcccttca attttgagat tttccagcgg cttgttgacg atgatcccca ttgcaccatt
     5341 ggtattatgt tcgcaaatgt agaccacgga acgacggaaa atcggatcct ggagagcagg
     5401 catggcaata agaaagtgat gctgtaaatt cattgtcaga ggttctgttt cctggttcaa
     5461 aaaagcaaca gcgcccagta tgcgtggaaa gaagggcgct gtcactcaga gtctcaacga
     5521 gatccttctc gctaaggtgg gttactgctg ctgtaaacgt gcttcgatgg catccattaa
     5581 cattccggtg atcgacaccg gaaactctgc ttcaatctca cgaatacagg ttgggctggt
     5641 gacgttaatt tcagtcagac ggtcgccgat gatatccaga ccaacaaaaa tcagcccttt
     5701 ttctttcagc gtcggcccga tctgacgggc gattttccag tcactttccg tcagcggacg
     5761 aggttcaccg cgaccaccgg cagccagatt gccacgggtt tcgcccccct gcggaatacg
     5821 cgccaggcag tacggtaccg gctcgccatc caccaccagc acgcgtttgt cgccatcttt
     5881 aatggctggc aggtaatttt gcgccatgca gtagcgagtg ccatgctcag tcagggtttc
     5941 ggcaatcacg ccgaggtttg gatcgccttc tttcacgcgg aaaatcgacg cgccgcccat
     6001 accgtccagc ggcttaagaa tgatgtcgct gtgtttctcc cagaacgctt ttagctgcgc
     6061 tttattgcgc gtaaccagcg tttctggcgt taagtcagag aaccaggcgg taaacagttt
     6121 ctcgttacag tcgcgcaggc tctgcggctt gttaacgatc agcgtccctt tctcttcggc
     6181 acgttccaga atataggtcg cgtagataaa ctcggtatca aacggcgggt ctttacgcat
     6241 caggatcaca tcgagatcgg ccagcggcag atcctgttca ccgacgaacg aaaaccactc
     6301 ttcgtagttc tgcttcacgt tcagcgtgcg ggtatgggcg cgggcttcgc cattgatcag
     6361 atacagatcg cccatctcca tatagtgaag ttcgtaacca cgacgctgtg cttccagcaa
     6421 catagcgaaa ctggaatctt tcttgatgtt gatgtttgcg atggggtcca tcacgatgcc
     6481 gagcttgatc attattcttc tccgttagcc caaatcgcca aatcgtactt gtagcgcggt
     6541 aatggcggtg agcgcagttg tctctgtacg caaaacgcga ggtcccaaca ggatatcagt
     6601 aaattgatag cgggcagtca tggcaatttc atctgccgat aaaccgcctt ccgggccaat
     6661 cagcaggcgg acgcgttcaa ccggtaacgg caacgtattg atgctgttac tggcgcgcgg
     6721 gtgaagattc agtttcagtc cttcatcctg ctctgcacac caggcttcca gatccatcgc
     6781 tggacggatt tccggcaccc ggttacgacc acactgctca caggcagcaa ttgcaatctt
     6841 ctgccactgc tggagcttct tgttcagacg ttcactatcc agtttaacgc cgcagcgctc
     6901 agaaaaaagt ggcgtaatga ggcttacacc gagttcgatc gatttctgga tcgtaaattc
     6961 cattttttca ccacgcgaca tcacctgacc gaggtgaata tgcagcggag attcgcgatc
     7021 gtcgatcttg ccttccagca ccttcacttc cacgcttttt ttgctggagc tggtaatttc
     7081 ggcgtcaaag acctggttgc taccgtcaaa caattgcaac gcctgccccg gccccatgcg
     7141 cagtacgcgc ccgatatggt tggcggcatc ttcgcaaagc gcgatgtgag aatggctggt
     7201 cagtggttca ggatgataaa tgcgggggat acgcatagtt aaaaatccgc gtcgtctccc
     7261 cacgcggttg tacgcgtggg gtaggggtta acaaaaagaa tcccgctagt gtaggttagc
     7321 tctttcgcgc ctggcaagcg cgttgcacat acgggttatg attgccttgc accttcgcga
     7381 tgcgttcatc gcgctcgcac tcccagtcgg taaccggata catcttgttc catgcgttga
     7441 acagctgcgt ttgctggcga gagagtgtca ggttgtattg gtcgcgcata tagaagtagg
     7501 tgcgcgcaat ggcaccgcgt gcacgcgctg gtggttcggc agctttttct ttgaaatcga
     7561 ccttcatggc gcattgaccg tactggcctt cgccgccatt ccactggctg tacataaagt
     7621 tgccgcgatc gccattcacc tcaccgactg acggctgcag gttatgcata tcgctttcca
     7681 tcttgcgata gaccggatct ttagcgcagt ttttacgtcc accgtcctgc cagcactggc
     7741 gctggtgacc gaactgccag gcgggaacga cgtgttccca ctctacgcgg ctggctcggt
     7801 tttcattttt gcgcacctga tagccgcacg attgcagatc aacaacgcct tttttgccct
     7861 gccagttaat tttacatccg caataaaacg taccgggcgc gtcagcgtgg acttttaccg
     7921 ccgcggcttt cgcctgagaa aaactattga taccttcggc caacgccggg ccggaaaatg
     7981 ctgcgctcag taccaccgca gcaatagaca aataacggta catcacttac tccgtgttaa
     8041 aacgagccag caacgtagcg aaagttgttt tggtatgcaa tcagccagat caggaaagtt
     8101 cctgatagtt cagatggtta ttttgcgacc agttgttcgc cgcagtgaac acagcgatag
     8161 acggcttcgc cacgaactac gcgattatgg cggcgtacgg taagctgatg ctcctggcac
     8221 ttgcagcggt aggggaaggt gttgcgacgc acggattgca gttcgaactg atgcgtacga
     8281 cgggcgggaa cacccagcac gttttccatc atccacttcc actctttgcc atgtggtgct
     8341 acgcgcccga aatgtttcca tgccagcaaa tgtgccagtt cgtgcggcac cacttcttca
     8401 ataaaggctt cactgttttc caacagcaaa acgggattga ggcggatttc atagctttcc
     8461 agccaggctg ttccggcgga ggtgccgcgc tgggtataag agagttttgg ctccgggtag
     8521 ttacgcccca atttcaggtt agcctgggcg agtttttccc gcaggcgacg cataacggcc
     8581 tgttggatgg cgatagggag acgggatgtt ttcatagcgg cagaggatag agcgaagaaa
     8641 agatgactgc aagaggcggc ttcctccgcg acgggaggaa gcctgaggag attaatcgtg
     8701 agcgccgatt tcgcgcagtt tacgaccttt catcagatta cgttcaatat gttccagcga
     8761 aacgtgtttg gtttccggta ccagccacaa tgttagcagg ataaacaata cgttcagagc
     8821 cgcatacacc cagaaggtgt tggcgttacc cagcgtgttg agcatggtca ggaacgttgc
     8881 gccaacgatc atgttggcaa tccagttggt ggcggtggag caggtgatgc caaaatcgcg
     8941 gcctttcagc ggctgaattt cggagcacag tacccaaatc agcggaccgg cactcatggc
     9001 aaaaccgaca ataaacatca gcagcatggc gatggcgaaa tactgcgctg acggagagtg
     9061 aataccgata tgcatcattg taccgagtac gcccatgcca gcagccatca ccaggaagcc
     9121 cagcgttagc gttggttttc gtccccagcg gtcaacaagg ccgattgcga taaaggtggc
     9181 aagtacgttg gtcaggccga caatcactgt cccccacatt tgctctgtgg tgttggtata
     9241 acccgccagt tcgaagattt tcggcgcgta atacatgatg acgttcatcc cggtgaattg
     9301 ctgcattacc tgcaacagta cgccaaggaa caccgcgcgg cggaagttgc tgttctcttt
     9361 aaacagcgcc cagccactct gtttaacctg caaactttca cggatttcat ccagttcgcg
     9421 tttcgcttcc gcgctggtgt cacgcaggcg tagcagcacg cgttcggcat caacaaaacg
     9481 gcgtttggcg gcaaaccaac gtgggctgtc tggcaggaag aagacaccaa tcagcagcaa
     9541 aattgccggg atgataatca cacccagcat ccagcgccat gcaccggtgt agctgaaggc
     9601 tgtatcagaa agataagcac cgaggatccc gatagtgatc atcaactgat acatcgagat
     9661 catactgcca cgaatttttt ccggcgcaat ttcagagagg tacagcggtg cggtataaga
     9721 ggccacaccc accgccagcc ccagcagaac gcgggaaaga atcagtactt caacgtttgg
     9781 cgcagccgca gagaacagtg aaccggcaac aaacaggatt gcgccgatca tcaggctctt
     9841 tttgcgcccg agtttaaagg agagccagcc gctgcccacc gcaccgactg ccgcaccgaa
     9901 catcatggag cttacgaccc attcttgcgt gtgcgaagta atctggaatt catctgcaat
     9961 aaacggcagt gcgccagcaa ttacaccgat atccaggcca aagagtaatc ccgccagagc
    10021 ggcaaggaag cagacgaaaa acgtcattgc cttgtttgac cgcccctgtt ttttagcgtc
    10081 aggcatgatg ccctccaata tggttatttt ttattgtgaa ttaagatagg tgagtacgac
    10141 gtaaaaagat gtgaagcaaa tcacatcagt gtaatcgctt acactaacat ttatcttagt
    10201 tatggtttaa gtgcatgaaa ataattgagt tatatcatca tagattaaac gctgttatct
    10261 gcaattaaga ctttactgaa aagaaatgta acaactgtga aaactgaata ataatatgaa
    10321 aatattacaa atatttggct ggtgttgttg gtgtaatcgg tttcagtagt gatgataatt
    10381 tgcgataaaa cgtgttgagg aagaatgatg ttattgtgcg ggcagctttt tgccggatgc
    10441 ggcgtgaatg cctatccacc gcggtgaggt aaaagaaaaa ggccagcgcg aggctggcct
    10501 ttgaaagcag gtgaagaaag attacttcag accggcagca tcgcgcagca gctgcgcttt
    10561 gtcggttttt tcccacggga aatgttcacg accaaagtga ccgtatgctg cggtttcttt
    10621 gtagatcggg tgcagcagat ccagcatctg aatcagaccg tatgggcgca ggtcgaagaa
    10681 ctcacgtacc agcagggtca gctgttcaga aggcactttc tcagtaccga aagtttctac
    10741 catgatggag gtcggttcag ccacgccgat tgcgtaggaa acctgaattt cacaacgatc
    10801 ggccaggcca gcagcaacga tgtttttcgc gacataacgt gctgcgtagg ctgcggaacg
    10861 gtccactttt gatggatctt taccagagaa tgcaccgcca ccgtgacgtg ccatgccgcc
    10921 gtaggtatca acgataattt tacgaccagt cagaccgcag tcacccatcg ggccaccgat
    10981 aacgaaacga ccagtcgggt tgatgaagaa tttggtggca gaagtcagcc attcagcggg
    11041 cagaattggc ttgatgatct cttccattac cgcttcttgc agcgatttct ggtcgatctc
    11101 ttcagagtgc tgagtggaaa gcacgacagc atcaatacca acgattttgc cgtcgtcata
    11161 ctggaaagtc acctggcttt tcgcgtccgg gcgcagccat ggcagagtgc cgtttttacg
    11221 cacttcagcc tgacgctgta ccagacggtg tgcataggtg ataggtgctg gcatcagcac
    11281 gtcggtttca ttagttgcgt agccaaacat cagaccctgg tcacccgcgc cctgttccag
    11341 cggatcggca cggtcaacgc cctggttgat gtcaggagac tgtttgccga tagcgctcag
    11401 aaccgcacag gagttagcgt caaagcccat gtcggaatgc acatagccaa tttcgcgaac
    11461 ggtgttacgg gtgatctctt cgatgtctac ccaggcgctg gtggtgattt cgccgccaac
    11521 taaaaccatg ccggttttta cgtaggtttc gcaagcaacg cgtgctttcg gatcctgttc
    11581 gaggatcgcg tctaaaacgg catcagagat ttggtcagca attttgtcag gatgcccttc
    11641 agagacggac tcggacgtaa aaaggtgttt tgccatattt aatatcacct aaagagaatt
    11701 tggttagctc aaactgttgt gtggattttc tgtggtagcg gatcctacca cgactctgca
    11761 ggttaaaaac actggcagtc tgagtgttaa tcggtatgga tggattaaca tctggatggc
    11821 tattttaggt caattcttca cccgatttcc actttttttt gaatcgtgtc tcattctgtt
    11881 aaaaacgtgg ctggaaattt ttcctgacaa tgccggcatt ctgcgtattt atcttttgca
    11941 attttctgcc attgtggggt ataaaacgcg gcgcgcggct taaataaaaa gcacacgacg
    12001 tttctttcgt gttgccactt ccagccgggt tgaaatcaga gttttggctt gtgggttcgt
    12061 cttaacaggc ggccgtggag gtgatacgaa ataatgaacc gttgtctgct gcttaacctg
    12121 tctcaccgtt ctggtgaaga ttcgttcccc gcactctgca tctctgcttt gcatacctgc
    12181 cgatgttata cccatctcgg cgcttctcag gattcaagag ctggttacag ttactgagga
    12241 ctgaacaagg gcgctcttgt aaaaacaaga gttttctcgt ggtttcgccg aactgtcaca
    12301 cttacgttcg gttatgtgct taataatgtt atgaaaaaga aaccggttgc gcagttggag
    12361 cgtcagcatt cactgctgga aaatccatgt gcttatgggt tgttatcgca gttccaggct
    12421 gcgatagtcg ttaactgttt tacacttaat aaaataattt gaggttcgct atgtctgacg
    12481 acatgtctat gggtttgcct tcgtcagcgg gcgaacacgg tgtactacgc tccatgcagg
    12541 aggttgcaat gagctcccag gaagccagca agatgctgcg tacttacaat attgcctggt
    12601 ggggcaataa ctactatgac gttaacgagc tgggccacat cagcgtgtgc ccggacccgg
    12661 acgtcccgga agctcgcgtc gatctcgcgc agttagtgaa aactcgtgaa gcacaggggc
    12721 agcgtctgcc tgcactgttc tgtttcccac agatcctgca gcaccgtttg cgttccatta
    12781 acgccgcgtt caaacgtgcg agggaatcct acggctataa cggcgattac ttccttgttt
    12841 atccgatcaa agttaaccag caccgccgcg tgattgagtc cctgattcat tcgggcgaac
    12901 cgctgggtct ggaagccggt tccaaagccg agttgatggc agtactggca catgctggca
    12961 tgacccgtag cgtcatcgtc tgcaacggtt ataaagaccg cgaatatatc cgcctggcat
    13021 taattggcga gaagatgggg cacaaggtct atctggtcat tgagaagatg tcagaaatcg
    13081 ccattgtgct ggatgaagca gaacgtctga atgtcgttcc tcgtctgggc gtgcgtgcac
    13141 gtctggcttc gcagggctcg ggtaaatggc agtcctccgg cggggaaaaa tcgaagttcg
    13201 gcctggctgc gactcaggta ctgcaactgg ttgaaaccct gcgtgaagcc gggcgtctcg
    13261 acagcctgca actactgcac ttccacctcg gttcgcagat ggcgaatatt cgcgatatcg
    13321 ccacgggcgt tcgtgaatcc gcgcgtttct atgtggaact gcacaagctg ggcgtcaata
    13381 ttcagtgctt cgacgtcggc ggcggtctgg gcgtggacta tgaaggcacc cgttcgcagt
    13441 ctgactgctc cgtaaactat ggcctcaatg aatacgccaa caacatcatc tgggcgattg
    13501 gcgatgcgtg tgaagaaaat ggtctgccgc atccgacggt aatcaccgaa tcgggccgtg
    13561 cggtgactgc gcaccatacc gtgctggtgt cgaatatcat cggcgtggaa cgtaacgaat
    13621 atacggtgcc gaccgcgccc gcagaagatg cgccgcgcgc gctgcaaagc atgtgggaaa
    13681 cctggcagga aatgcacgaa ccgggaactc gccgttctct gcgtgaatgg ttacacgaca
    13741 gccagatgga tctgcacgac attcatatcg gctactcttc cggcaccttt agcctgcaag
    13801 aacgtgcatg ggcagaacaa ctctatctga gcatgtgcca tgaagtgcag aagcaactgg
    13861 atccgcaaaa ccgtgcgcat cgtccgataa tcgacgaact gcaggaacgt atggcggaca
    13921 aaatgtacgt caatttctcg ctgttccagt caatgccgga cgcatggggg atcgaccagt
    13981 tgttcccggt tctgccgctg gaagggctgg atcaagtgcc ggaacgtcgc gcagtgctgc
    14041 tggatattac ctgtgactct gacggtgcta tcgaccacta tattgatggt gatggtattg
    14101 ccacgacaat gccaatgccg gagtacgatc cagagaatcc gccgatgctc ggtttcttta
    14161 tggtcggcgc atatcaggag atccttggca atatgcacaa cctgttcggt gataccgaag
    14221 cggttgacgt gttcgtgttc cctgacggta gcgtagaagt agaactgtct gacgaaggcg
    14281 ataccgtggc ggacatgctg caatatgtac agctcgatcc gaaaacgctg ttaacccagt
    14341 tccgcgatca agtgaagaaa accgatcttg atgctgaact gcaacaacag ttccttgaag
    14401 agttcgaggc aggtttgtac ggttatactt atcttgaaga tgagtaagtc ctgtgttact
    14461 tgaatccgct taatttagcg gtgataatcc gccacaattt attgtgacaa atccaaccct
    14521 tcctcgtcgg gcctaacgac gcggaagggt ttttttatat cgactttgta ataggagtcc
    14581 atccatgagc accttaggtc atcaatacga taactcactg gtttccaacg cctttggttt
    14641 tttacgcctg ccgatgaact tccagccgta tgacagcgat gctgactggg tgattactgg
    14701 tgtgccgttc gatatggcca cttctggtcg tgcgggggga cgccacggtc cggcagcgat
    14761 ccgtcaggtt tcgacgaatc ttgcctggga acacaatcgc ttcccgtgga atttcgacat
    14821 gcgtgagcgc ctgaacgtcg tggactgcgg cgatctggta tatgcctttg gcgatgcccg
    14881 tgagatgagc gaaaaactgc aggcacacgc cgagaagctg ctggctgccg gtaagcgtat
    14941 gctctctttc ggtggtgacc actttgttac gctgccgctg ctgcgtgctc atgcgaagca
    15001 tttcggcaaa atggcgctgg tacactttga cgcccacacc gatacctacg cgaacggttg
    15061 tgaatttgac cacggcacca tgttctatac cgcgccgaaa gaaggtctga tcgacccgaa
    15121 tcattccgtg cagattggta ttcgtaccga gtttgataaa gacaacggtt ttaccgtgct
    15181 ggacgcctgc caggtgaacg atcgcagcgt ggatgacgtt atcgcccaag tgaaacagat
    15241 tgtgggtgat atgccggttt acctgacctt tgatatcgac tgcctggatc ctgctttcgc
    15301 gccgggaacc ggtacgccag tgattggcgg cctgacctcc gatcgcgcca ttaaactggt
    15361 acgcggcctg aaagatctca acattgttgg gatggacgta gtggaagtgg ctccggcata
    15421 cgatcagtcg gaaatcaccg ctctggctgc ggcgacgctg gcgctggaga tgctgtatat
    15481 tcaggcggcg aaaaagggcg agtaagcacc agatgcgatg cgcacgggta aaacgtgcca
    15541 ttaatgtcgg atgcggcgtg aacgccttat ccgacctacg ttcggcaccc gtaggccgga
    15601 taagatgcgc cagcatcgca tccggcaatg cgcacaaggt aacaaatgtg ccattcatgt
    15661 cagatgcggc gtgaacgcct tatctgacct acgttcgaca ccaccaggct ttacttaaca
    15721 ccatccgcgc tcatgcgatc gcgaatatgc tgggcgcgtt cggcggatgc aggatggtcg
    15781 tcaaacattg agctttggcg gccttcttcc agtttcgcca gtttttcaaa gctggtggca
    15841 agacctgccg ggctgatgcc gcgttggcgc agaagatcgt aagaataatc atcggcttct
    15901 gcttcctggc gctgggagaa ttgcgaatta actaatttct cgcccagatc accgagttgt
    15961 gattgagata aactgccgac aatcccgccc gcagaggccg cagctactcg cacggcattt
    16021 gtaccaagtg ccacctgcat tccttttttc acatggccta atgccacgtg ccccatttcg
    16081 tgaccgatca ccgcttcgac ttcgttatcc gtcatcatat ccatcagccc gctatagaca
    16141 cggatacagc cgttagccat tgcaaaggcg ttcacatcct tcgccatata cactttgtaa
    16201 tttaccggct gaccgttgat attgttgcct agcgcattgg caatagttgt cagacgttta
    16261 gcgtattcgc tattggctgg cgcaatcgtc gccttgctgt ccatctcctg acatgcctga
    16321 tcgctcaggg ctttcacctg cgcatcactc aaactgtaag cctgaaaagc ttccgctcct
    16381 gatgagagca gtccgttgga gtccatattc tggcaaccag tcagtaccgt tgccacgctc
    16441 attgctacca ataaggcgcg aattttcatg ttttctcttc cgcacaaggt caggattatt
    16501 ctaaaaatgc gccgtttgca ggtgaatcga cgctcagtct cagtataagg aaaagcgcag
    16561 cggacgggcg agtagattgc gcaacatgcg agcatgatcc agagatttct gaagcggcaa
    16621 aaggatgttc catgtacatg acgcgcggct tgcggtaaat tgttggcaaa ttttccggcg
    16681 tagcccaaaa cgcgctgtcg tcaagtcgtt aagggcgtgc ccttcatcat ccgatctgga
    16741 gtcaaaatgt cctcacgtaa agagcttgcc aatgctattc gtgcgctgag catggacgca
    16801 gtacagaaag ccaaatccgg tcacccgggt gcccctatgg gtatggctga cattgccgaa
    16861 gtcctgtggc gtgatttcct gaaacacaac ccgcagaatc cgtcctgggc tgaccgtgac
    16921 cgcttcgtgc tgtccaacgg ccacggctcc atgctgatct acagcctgct gcacctcacc
    16981 ggttacgatc tgccgatgga agaactgaaa aacttccgtc agctgcactc taaaactccg
    17041 ggccacccgg aagtaggtta taccgctggt gtggaaacca ccaccggtcc gctgggtcag
    17101 ggtattgcca acgcagtcgg tatggcgatt gcagaaaaaa cgctggcggc gcagtttaac
    17161 cgtccgggcc acgacattgt cgaccactac acctacgcct tcatgggcga cggctgcatg
    17221 atggaaggca tctcccacga agtttgctct ctggcgggta cgctgaagct gggtaaactg
    17281 attgcattct acgatgacaa cggtatttct atcgatggtc acgttgaagg ctggttcacc
    17341 gacgacaccg caatgcgttt cgaagcttac ggctggcacg ttattcgcga catcgacggt
    17401 catgacgcgg catccatcaa acgcgcagta gaagaagcgc gcgcagtgac tgacaaaccg
    17461 tccctgctta tgtgcaaaac catcatcggt ttcggttccc cgaacaaagc cggtacccac
    17521 gactcccacg gtgcgccgct gggcgacgct gaaattgccc tgacccgcga acagctgggc
    17581 tggaaatacg cgccgttcga aatcccgtct gaaatctatg ctcagtggga tgcgaaagaa
    17641 gcaggccagg cgaaagaatc cgcatggaac gagaaattcg ctgcttacgc gaaagcttat
    17701 ccgcaggaag ccgctgaatt tacccgccgt atgaaaggcg aaatgccgtc tgacttcgac
    17761 gcgaaagcaa aagagttcat tgctaaactg caggctaacc cggcgaaaat cgccagccgt
    17821 aaagcgtctc agaatgctat cgaagctttc ggtccgctgt tgccggaatt cctcggcggt
    17881 tctgctgacc tggcaccgtc taacctgacc ctgtggtctg gttctaaagc aatcaacgaa
    17941 gatgctgcgg gtaactacat ccactacggt gttcgcgagt tcggtatgac cgcgattgct
    18001 aacggtatct ccctgcacgg tggtttcctg ccgtacacct ccaccttcct gatgttcgtg
    18061 gaatacgcac gtaacgccgt acgtatggct gcgctgatga aacagcgtca ggtgatggtt
    18121 tacacccacg actccatcgg tctgggcgaa gacggcccga ctcaccagcc ggttgagcag
    18181 gtcgcttctc tgcgcgtaac cccgaacatg tctacatggc gtccgtgtga ccaggttgaa
    18241 tccgcggtcg cgtggaaata cggtgttgag cgtcaggacg gcccgaccgc actgatcctc
    18301 tcccgtcaga acctggcgca gcaggaacga actgaagagc aactggcaaa catcgtgcgc
    18361 ggtggttatg tgctgaaaga ctgcgccggt cagccggaac tgattttcat cgctaccggt
    18421 tcagaagttg aactggctgt tgctgcctac gaaaaactga ctgccgaagg cgtgaaagcg
    18481 cgcgtggtgt ccatgccgtc taccgacgca tttgacaagc aggatgctgc ttaccgtgaa
    18541 tccgtactgc cgaaagcggt tactgcacgc gttgctgtag aagcgggtat tgctgactac
    18601 tggtacaagt atgttggcct gaacggtgct atcgtcggta tgaccacctt cggtgaatct
    18661 gctccggcag agctgctgtt tgaagagttc ggcttcactg ttgataacgt tgttgcgaaa
    18721 gcaaaagaac tgctgtaatt agcatttcgg gtaaaaaggt cgcttcggcg acctttttta
    18781 ttaccttgat atgtccgttt gcggacaagc aatagataaa gcgtgctgta gatcacaaat
    18841 atttatatgc aataaatatc aattatgtaa tatgcatcac gatatgcgta ttgacatttg
    18901 ttgttatatc tataactcaa tgttatataa gaaattaata attcactgtt ttcaaaacac
    18961 cggtttccct gctcaattgc tttcattaaa ccgcacgagt attttatttt taccgccgga
    19021 cggtacccta aaaataaaat aaggataatt tatgcggctt agtgattatt ttccagaatc
    19081 atcaatctca gttatacatt cagcaaaaga ttggcaggaa gctatcgatt tctcgatggt
    19141 atcattgctg gataaaaact atatcagcga gaattacatt caggcaatta aagattccac
    19201 cattaacaat ggcccttatt atattctcgc accaggcgtg gcaatgcctc atgcgcgacc
    19261 ggaatgtggg gcgcttaaaa ccgggatgtc tttgacatta cttgaacaag gtgtttattt
    19321 tccggggaat gacgaaccaa ttaaattact catcggactc tctgctgccg atgccgattc
    19381 gcacattggc gctattcagg cgttaagtga attactgtgc gaagaagaaa tactcgaaca
    19441 actcttaaca gcatcatcag aaaaacaatt agcggacatt atcagccgcg gataattact
    19501 ctctcctcta caggataata ttatggaaaa caagtctgct cgtgcaaagg tccaagcttt
    19561 tgggggcttt ttgactgcaa tggtcatccc caatattggt gcttttattg cctggggttt
    19621 tattactgcg ttatttattc ccaccggttg gctgcctaac gaacatttcg ccaaaattgt
    19681 cggcccgatg attacctatt tattgcccgt gatgattggt tctacaggtg gtcatctggt
    19741 cggcggtaaa cgcggggcgg tcatgggcgg aataggtact attggtgtga tcgttggcgc
    19801 agagatcccg atgttccttg gctcaatgat catggggccg ctcggtgggt tggtcataaa
    19861 atatgtcgat aaggcactgg aaaaacgcat acctgccggt tttgagatgg ttatcaataa
    19921 cttctcatta ggtatcgcgg ggatgctcct ttgtctgctg ggttttgaag tgatcggccc
    19981 ggcggtgtta attgccaata ctttcgtcaa agagtgtatc gaggcgctgg tacatgcggg
    20041 ttatctgcct ctgctgtcag tcatcaatga accggcgaaa gtgcttttcc ttaataatgc
    20101 gatcgatcag ggcgtctatt atccgctggg aatgcaacag gcttcggtta acggtaaatc
    20161 catcttcttt atggtggcct ctaacccagg tccgggcctg gggctgctgc tggcgtttac
    20221 cttgtttggt aaagggatga gtaaacgttc tgcgcccggg gcgatgatta ttcacttcct
    20281 cggtgggatc cacgaactgt atttcccata tgtgctgatg aagccgctga ctattattgc
    20341 catgattgcg ggcggtatgt ctggtacctg gatgtttaac ttactggacg gtggtctggt
    20401 ggctggccca agtccggggt ctatctttgc ttacctggca ctgacgccga aaggctcgtt
    20461 cctggcgaca attgccggtg ttacggtagg taccctggtg tcctttgcta ttacttcgct
    20521 gatactgaag atggaaaaaa cggtggaaac ggagagcgaa gatgagtttg ctcagtcagc
    20581 caatgcggtt aaggcgatga aacaagaggg tgcattctcg ttaagcaggg ttaagcgtat
    20641 cgcctttgtt tgcgatgcgg ggatgggctc cagtgcgatg ggcgcgacca ccttccgtaa
    20701 acgcctggaa aaagcggggc tggcaataga agtaaaacat tacgcgatag aaaacgtgcc
    20761 tgcggatgcc gatatcgtcg tcactcacgc cagtctggaa gggcgcgtga aacgtgtgac
    20821 ggataaaccg ctgatattga ttaataacta tattggcgat ccaaaactcg acactttatt
    20881 taatcaatta accgccgaac ataaacactg attacagagg taaaaatgaa aaccaaagtt
    20941 gctgctattt atggcaagcg ggatgtccgt ctgcgcgaat ttgaactgcc agaaattacc
    21001 gataatgaac tgttagtgag tgtaatttct gacagcgtct gtttatcgac ctggaaagcg
    21061 gcgttactcg gtagtgaaca taaacgcgta cccgacgatt tagaaaatca tccggtcatt
    21121 accgggcatg aatgtgccgg ggttattgtc gaagtgggta aaaatctcac tggcaaatat
    21181 aaaaaaggcc agcgttttgt attgcaaccg gcgatggggt taccaagcgg atattcagca
    21241 ggctacagct acgaatattt tggcggtaac gccacctata tgattattcc cgaaatcgcc
    21301 attaatttgg gctgcgtatt accgtatcac ggctcttatt ttgctgcggc gtcgctggca
    21361 gagcctatgt gctgcattat tggtgcttat catgccaatt atcacaccac gcaatatgtt
    21421 tatgagcatc gcatgggcgt caaacctggc ggcaatattg cactgctggc gtgtgcgggt
    21481 ccgatgggca ttggcgctat cgattacgcc attaacggtg gcatacaacc gtcacgggtg
    21541 gtggtggtcg atatcgacga caaacgcctg gcgcaggtgc agaagctgct gccggtggat
    21601 ctggcggcca gtaaaggcat tgagctggtg tatgtgaata ccaaagggat gagcgatccg
    21661 gtccagacgc tgcgggcgct gacaggagat gtcgggttcg atgacatttt tgtttatgcg
    21721 gcggtgcctg ctgtcgttga gatggctgac gaattactgg cggaagatgg ctgtctgaac
    21781 ttctttgccg ggccgacgga taaaaacttc aaagtgccgt ttaatttcta caacgtccat
    21841 tacaacagca cgcacgtagt cggtacatcc ggcggttcaa cggacgacat gaaagaggcg
    21901 attgcgttaa gcgccacggg gcagttgcag ccgtccttta tggtcacgca tatcggtggg
    21961 ctggatgcgg tgccagatac cgtgctcaat ctgccggata tccctggcgg taaaaaactc
    22021 atttataacg gcgtgaccat gccgctcacc gccattgccg attttgccga aaaaggcaaa
    22081 accgatccga tgtttaaaga gctggcgcgg ctggttgagg aaacgcacgg catctggaat
    22141 gagcaggccg agaaatatct gctggcgcaa tttggcgttg atattgggga ggccgcgcaa
    22201 tgatgtccct ggcgtggcca ttatttcgcg ttacggaaca ggcagcgttg gctgcctggc
    22261 cgcaaaccgg atgtggcgac aaaaataaaa ttgatggcct ggcggtcacc gcgatgcgcc
    22321 aggcattaaa cgacgtcgct tttcgtggac gagtggttat cggcgaagga gaaattgacc
    22381 atgcgccgat gctgtggatt ggcgaagagg tgggcaaagg cgatgggcca gaagtcgata
    22441 tcgcggttga tcccattgaa ggcacgcgga tggtggcgat ggggcagagc aatgcgctgg
    22501 cggtcatggc tttcgcccct cgggatagcc tgttacatgc gcccgatatg tatatgaaaa
    22561 agctggttgt taatcgactg gctgccgggg tgattgatct gtctttgcct ctggcggaca
    22621 acttgcgtaa cgtggcgcgg gcgttaggta aaccgctgga caagctgcgc atggttacgc
    22681 tggataaacc gcgcctgagt gccgcgattg aggaagcaac acagctgggc gtgaaggttt
    22741 ttgccctgcc ggatggcgat gtcgccgcca gcgtgctgac ctgctggcag gataatcctt
    22801 acgatgtgat gtacaccatc ggcggtgcgc cagaaggcgt gatttctgcc tgtgccgtta
    22861 aagcattagg cggggatatg caggccgagc tgattgactt ttgtcaggct aaaggggatt
    22921 acacggaaaa tcggcagatt gcagagcagg agagcaagcg ttgcaaggca atgggcgttg
    22981 acgttaaccg tgtgtactcg ctcgatgaac tggtgagggg gaacgatatc ctctttagcg
    23041 ctacgggcgt gacgggcggc gagttggtga acggtatcca acagacggcg aatggggtgc
    23101 ggacgcagac attactgatc ggcggcgcgg accaaacgtg taatataata gactccctgc
    23161 attgatggtg atctatcgga agaccaatgg cgaagctgac agaagatgat gtgcttgagc
    23221 aactggatgc acaggacaat ttattttcat ttatgaaaac tgcgcattct attttgctcc
    23281 aggggatacg ccagtttctg ccgttgctgt ttgtcgataa cgatgaagag atcgtggaat
    23341 atgcagtgaa gccgttactc gcccaaagcg gcccgcttga cgatattgat gttgcgctgc
    23401 gtttgattta tgcgctgggg aaaatggata aatggctgta cgccgatatc acgcattttt
    23461 cccagttctg gcattacctg aacgaacagg atgaaacgcc cggatttgcc gatgacatga
    23521 cctgggattt tatcagcaac gtcaatagca taacccgcaa tgcgatgctc tatgacgcgt
    23581 tgaaagcgat gaagttcgcc gatttcgccg tctggtcaga ggcgcgtttt agcgggatgg
    23641 tcaaaacggc gctgacgctg gcagtaacga caaccttaaa ggaattaacg ccgtgaaaat
    23701 tgaattagcg gtgaatgggc tgaagataca ggcacagtac cagaacgaag agattgaaaa
    23761 tgtacataaa ccgttgctgc gtatgctggc ggccttacag acggtaaatc cgcagcggcg
    23821 tacggtggtt tttctttgcg ctccgccggg gacgggtaaa tctaccctaa ccaccttctg
    23881 ggaatatctc gcgcaacaag acccggagct gcccgctatt cagacgctcc cgatggatgg
    23941 ttttcaccat tacaatagct ggctggacgc gcatcaattg cgccccttca aaggcgcacc
    24001 agagacattt gacgttgcga aactggcgga aaatctgcgc caggtggtgg aaggggattg
    24061 tacgtggccg cagtacgatc gacaaaagca tgatcctgtt gaagatgcgt tgcacgttac
    24121 cgcaccactc gtcatcgtcg aaggaaactg gttgttgctg gacgacgaga agtggtgcca
    24181 gcttgcgcaa ttttgtgatt tctcgatctt tatcaacgcg ccagctactg cgttgcggga
    24241 gcgtctggtg gggcgcaagc tggcgggagg ggtatcgctg gcagatgcag aagcctttta
    24301 tgaccgtacc gacgggccga atgttcgccg ggtgctggaa gagagtctgc ctgcaaattt
    24361 gaccttaatg atgaccgcta cgggtgaata tcgtctggtg gattaacgtt cctgattaca
    24421 ccatcttcca gtcgcaccac acgagagaaa tgacgccggg taaatgcggc gtcatgactg
    24481 attgctacca cgctcgtgcc tcgctggcgg cacttctcca gccagctttc aaacacgctc
    24541 aaccaatttt cgtcaaaatc ccgactcggc tcgtccagca gtaatagtgg cggagagagt
    24601 gcttccaggc aggcgacggc gaccatccgt cgctgcgcac tatgcagatc caacgggtgc
    24661 gagttggcag tgtctgctaa ctgacaacat tgcagggcag catgggtacg ttgagtgatt
    24721 tcatccgccg ggcatttttg cagtttcaaa ccaaatgcga tctcgtcagc gacggtgctg
    24781 tgaaaaagtt ggttttccgc ttcctgaaac agtacgccaa cctttgccgc gcgctggcgg
    24841 tttttcaggt ttttcatcgc ctgttgttgc agcataaccg taccggctgt gggcgtaaga
    24901 agacctgcca ttactcgtaa cagcgtcgat ttccctgcgc cattgtcacc ggtcagcgcc
    24961 aaccattcac cttgtttgag ttgcagagaa atgtcgcaaa gacaatccgt tgccgcgcca
    25021 ggccagcgat aagatatctg atttaacgtt aacatagtgg ctggagcgct ccgttttgca
    25081 attgccacgc ttgctggcag tagttcagga aaggtgtgcg atgacgttca aaaagaataa
    25141 tcagcgaacc acgttcaaac gcccagtgtt gtaagcgctg caacagcatt tcgctggcct
    25201 gcggcgtcag gcggctaaag gcttcatcaa gaattaagag ctttggctgc atcgcgatgg
    25261 cgcaggcgat gaccacgcgc tgtgtctctc caccggagag tgttgccggg tgacgatggc
    25321 gcaacggctg gcactcggtc agagtcaggg cggcatcaat ccgcgccata atctctgctt
    25381 cagcaagaca cagattttct ggcccaaagg caacttcttc ttccacgcta aaggtacagc
    25441 cggagagctg caaatagggc gattgctgga cgagctggat ggtggcggat tgttcattaa
    25501 gcgggagatg cccaatgggc gtaccgagga gcgtccccgt gcctgttatc tctcctggca
    25561 gaaagtccgg ataccagccc gccatcagct gtgccagcgt acttttgcca ctgccgttgt
    25621 caccaaagat ggcgaccatc cccggtttga catagtgaaa gtcataacag aacggaggat
    25681 gcgttgagtg tgtcgggcaa taacggaact gttctaacgt aaccatatcc acgctccgaa
    25741 ttctgtcagc atcagcaaga tcatggtgta acgcgctacg cgttgtaggg tgctatcggc
    25801 tggtgcccac aacgttgttc gtcggttgtg gatccgaaat gcccgcatat ccagcgccgc
    25861 accacggaca gccagatcgt taagcgcatt gtatgttagc ggaataatta acgcgggcat
    25921 cgcacgtaaa cgctgatacc agccctcatc cagcggcacg ccgcgggcac gctgcgcttc
    25981 atgaataatc gctaattgcc gttttaactg ctcaacgacc aataacggcc cggcaaacag
    26041 ataagcgacg cctggcggca ggcgagaagc aaacagagcg cgaataaaac attgcacggg
    26101 gacgtactgc atccacaact gtgaggttga aacaatcgcc agaatccgca accagagcgt
    26161 aatagcatgc gaccaccgtt ctggcgagcg tggcgtgccg cttagccatt ctggtagcca
    26221 gccaccgtgt accagccaca gcccggctcc cagcgagaac atcagccaga cgacatattt
    26281 tgcccgccgc cgcgtggcct ttagcgcaat aaggcagaaa aaggtcgccg cgctatagat
    26341 cggcaaaatg gtctgcgcgg gcaggataag cgtggtgcag gccgccaatg cccacagtgt
    26401 taatgaggta aatggatgca tttagcgcac ggcagccatt gccggaaaat ggcgcgtggt
    26461 gcgaatcggc agctggctca gtaaaagcca gacaatcacc gctgtaagaa ttttatcgac
    26521 caggttcgcg cctatcaccg taatcgccac cgacttcacc aggttttgcc ccatcgagtg
    26581 catccaggca acgaacaaat cggctccgct accggtgaca ccgccgaaca gcgcagtacg
    26641 cagcggtaca gcgacgaccg ttaccgcaag cgtaataatc acgccgctga caacgacttt
    26701 tggcagggtg cgaaaccaac ccgctcgcgc cagccaaccg gcgaccaggc cgatgaccat
    26761 cgcgacgggt gcaaatgcgg cggcgatcgg gtcggtcagc aatccccaga gtaaattggt
    26821 cagtaagccg gtcagcattc cgatcaccgg accaagcagt accgcgctaa taagtgtgcc
    26881 gatggagtcg agaaatatag gtaatttgac catgctggcc agttgcccgc cgatcatatt
    26941 gatggcgata gatataacga tcaacaccag tgcctgactg gaaaagtggc tacgcgccat
    27001 aagaaaacct ctttgaaagt agttatttaa cgggggaaat atgcaactcg tcgtaaccag
    27061 aaagcgcgca aggttggcgg ctaaacgcca gttcctcaag gctaccgatc accagttcaa
    27121 gcgtggtccg cacggtacag ccgggcatca tatgaccgcc aagcatcgtg ccgtgtggat
    27181 cggaaacgca gagatgaaga tgctcaccac tctgttccag cgtgccgttt agtgagatca
    27241 cttcgaatgt accgttcagg agcgtggtgc cttcttgccc ggcatagcgc aaggcgacgt
    27301 cagtcaggct accggtacag cctgcaatcc aggcggcatg aagttgatgc tgctgtgcaa
    27361 aagcgtgtag ctgggaaaaa cttcctgccc ggggaggagg cgcagtgcgt aaaagcgtgc
    27421 ggaggaagaa tatgggtagg gcaatgtcat cattggagta ctccgaaact cggacgtatt
    27481 gcggataata tcgacgctgc atcagtcata ttctgatata agtcagggtt tgtgacggca
    27541 caacgaaaag attttgctac gcttattcgt gattctgcaa agtggaataa attccacagg
    27601 ggcggggctg aaaacgtgac gtaagtcaga taattgcttt ctttgactgg acaatcattc
    27661 cttttattcc acgtttcgct tatcctagct gaagcgtttc agtcgattaa atgttcgaca
    27721 attaaccaat cagtcgcagt ttgcgacagg taaggtttcc ccggacgatt tgctggatta
    27781 ctctgtctgg caaatttgct cagggaaaac cttgcaggag atctatgacc gtacgcgtag
    27841 cgataaatgg cttcggtcgc atcgggcgta atgtggttcg tgctttgtat gaatccggac
    27901 gccgggcgga aattaccgtg gtggcaatca acgaactggc ggatgctgcg ggcatggcgc
    27961 atttgttgaa atatgacacc agccatggcc gttttgcatg ggaagtacga caggaacgcg
    28021 atcaactttt tgttggtgat gacgccatcc gcgtattgca tgaacgttca ctgcaatcgc
    28081 tcccctggcg tgaacttggc gttgatgtag tcctcgactg caccggcgta tatggctccc
    28141 gcgagcatgg cgaagcgcat attgccgccg gggccaaaaa agtgctcttt tcacatcctg
    28201 gcagtaacga tctcgacgcg accgttgttt acggcgtcaa tcaggagcaa cttcgtgcgg
    28261 aacaccgcat cgtttctaac gcttcctgta ccacgaattg cataattccc gtcatcaaat
    28321 tgttagatga tgcgtacggt attgagtccg gcactgtgac cacaattcac tccgccatgc
    28381 acgatcaaca ggttattgat gcataccatc ctgacctgcg tcgcacccgg gcagccagcc
    28441 agtcgatcat tccggtcgat actaaactgg ccgccggtat cacacgattt tttccgcaat
    28501 ttaacgatcg ctttgaagcg attgcggtac gtgtgccaac cataaatgtg acggcaatcg
    28561 atttaagcgt gacggtgaag aaacctgtaa aagccaatga agtcaacctg ttgctgcaaa
    28621 aagcagcaca aggtgcattt catggtatag ttgactatac ggaattgccg ttggtctctg
    28681 tagattttaa ccacgatccg cacagtgcca ttgtcgatgg cacccaaacc cgggtcagtg
    28741 gcgcacacct gatcaaaacg ttggtctggt gcgataacga atggggcttt gctaaccgaa
    28801 tgctcgacac gacgttagct atggctactg ttgctttcag gtaagacgca agcagcgtct
    28861 gcaaaacttt tagaatcaac gagaggattc accatgtctg taattaagat gaccgatctg
    28921 gatcttgctg ggaaacgtgt atttatccgt gcggatctga acgtaccagt aaaagacggg
    28981 aaagtaacca gcgacgcgcg tatccgtgct tctctgccga ccatcgaact ggccctgaaa
    29041 caaggcgcaa aagtgatggt aacttcccac ctgggtcgtc ctaccgaagg cgagtacaac
    29101 gaagaattct ctctgctgcc ggttgttaac tacctgaaag acaaactgtc taacccggtt
    29161 cgtctggtta aagattacct cgacggcgtt gacgttgctg aaggtgaact ggttgttctg
    29221 gaaaacgttc gcttcaacaa aggcgagaag aaagacgacg aaaccctgtc caaaaaatac
    29281 gctgcactgt gtgacgtgtt cgtaatggac gcattcggta ctgctcaccg cgcgcaggct
    29341 tctactcacg gtatcggtaa attcgctgac gttgcgtgcg caggcccgct gctggcagct
    29401 gaactggacg cgctgggtaa agcactgaaa gaacctgctc gcccgatggt ggctatcgtt
    29461 ggtggttcta aagtatctac caaactgacc gttttggact ccctgtctaa aatcgctgac
    29521 cagctgattg ttggtggtgg tatcgctaac acctttatcg cggcacaagg ccacgatgtg
    29581 ggtaaatccc tgtacgaagc tgacctggtt gacgaagcta aacgtctgct gaccacctgc
    29641 aacatcccgg ttccgtctga tgttcgcgta gcaaccgagt tctctgaaac tgcaccggct
    29701 accctgaaat ctgttaacga tgtgaaagct gacgagcaga tcctggatat cggtgatgct
    29761 tccgctcagg aactggctga aatcctgaag aatgcgaaaa ccattctgtg gaacggtccg
    29821 gttggcgtgt tcgaattccc gaacttccgc aaaggtactg aaatcgtggc taacgctatc
    29881 gcagacagcg aagcgttctc catcgctggc ggcggcgaca ctctggcagc aatcgacctg
    29941 ttcggcattg ctgacaaaat ctcctacatc tccactggcg gcggcgcatt cctcgaattc
    30001 gtggaaggta aagtactgcc tgcagtagcg atgctcgaag agcgcgctaa gaagtaaaaa
    30061 atcacagggc agggaaacct gcccttgttt cagcgcgctt ttagagcacg cattttttca
    30121 tactctaaat aattcgagtt gcaggaaggc gacaagagag tgaatcccca ggagcttaca
    30181 taagtaagtg actggggtga gcgaacgcag acgcagcaca tgcaacttga agtatgatga
    30241 gtataaggcc cgacgataca ggacaagaga catgtctaag atttttgatt tcgtaaaacc
    30301 tggcgtaatc actggtgatg acgtacagaa agttttccag gtagcaaaag aaaacaactt
    30361 cgcactgcca gcagtaaact gcgtcggtac tgactccatc aacgccgtac tggaaactgc
    30421 tgctaaagtt aaagcgccgg ttatcgttca gttctccaac ggtggtgctt cctttatcgc
    30481 tggtaaaggc gtgaaatctg acgttccgca gggtgctgct atcctgggcg cgatctctgg
    30541 tgcgcatcac gttcaccaga tggctgaaca ttatggtgtt ccggttatcc tgcacactga
    30601 ccactgcgcg aagaaactgc tgccgtggat cgacggtctg ctggacgcgg gtgaaaaaca
    30661 cttcgcagct accggtaagc cgctgttctc ttctcacatg atcgacctgt ctgaagaatc
    30721 tctgcaagag aacatcgaaa tctgctctaa atacctggag cgtatgtcca aaatcggcat
    30781 gactctggaa atcgaactgg gttgcaccgg tggtgaagaa gacggcgtgg acaacagcca
    30841 catggacgct tctgcactgt acacccagcc ggaagacgtt gattacgcat acaccgagct
    30901 gagcaaaatc agcccgcgtt tcaccatcgc tgcgtccttc ggtaacgtac acggtgttta
    30961 caagccgggt aacgtggttc tgactccgac catcctgcgt gattctcagg aatatgtttc
    31021 taagaaacac aacctgccgc acaacagcct gaacttcgta ttccacggtg gttccggttc
    31081 tactgctcag gaaatcaaag actccgtaag ctacggcgta gtgaaaatga acatcgatac
    31141 cgatacccaa tgggcaacct gggaaggcgt tctgaactac tacaaagcga acgaagctta
    31201 tctgcagggg cagctgggta acccgaaagg cgaagatcag ccgaacaaga aatactacga
    31261 tccgcgcgta tggctgcgtg ccggtcagac ttcgatgatc gctcgtctgg aaaaagcatt
    31321 ccaggaactg aacgcgatcg acgttctgta agatattcct ttctgcttat ctcaaggccc
    31381 gctctgcggg tctttttttc gccaaaagca aaattgcctg atgcgctacg cttatcaggc
    31441 ctacacgtat cctgcaatat attgaatttg caggtttttg taggccggat aaggcgttca
    31501 cgccgcatcc ggcatgacaa agcgcacatt gatatctgtc tgagccccgc tctgcgggga
    31561 ttttttcgcc aaaagcaagg tgattcagat gagaaatctg tgatctattt ggcaaaatta
    31621 tgctttattg tttacccttg tcagactgcc cgtcataagg cggcggagtg tatttctcca
    31681 ttttgagtca gttgaaaagg aatattgaat ggaagatttg aatgttgtcg atagcataaa
    31741 cggcgcggga agctggctgg tagctaacca ggcgctgctg ctaagttatg cagtaaacat
    31801 cgtggcggca ctcgcgatca tcatcgttgg tttgattatc gcgcggatga tttccaacgc
    31861 ggtgaatcgc ctgatgatct cccgtaaaat cgatgccact gttgctgatt ttctttctgc
    31921 attagtccgt tacggtatta tcgcctttac gctaatcgct gccctgggac gcgtgggtgt
    31981 acaaacagcg tcagtcattg ctgtactcgg tgccgcaggc ttagctgttg gtctggcttt
    32041 gcaggggtca ctttctaacc tggccgctgg tgtgttactt gtcatgttcc gcccgttccg
    32101 tgccggagaa tatgttgacc tcggcggcgt agccggtact gtgctgagtg tgcagatttt
    32161 ctccaccacc atgcgtactg cagacggtaa aattatcgtt attccgaacg gtaaaattat
    32221 tgccggaaat attattaact tctcccgcga gccagttcgc cgtaacgaat ttattattgg
    32281 cgtggcgtat gattccgata tcgatcaggt taagcagatc ctgaccaata ttatccagtc
    32341 tgaagatcgc attttgaaag atcgcgaaat gactgtgcgc ctgaacgaac ttggtgcatc
    32401 gtcgattaat ttcgtggtcc gcgtctggag caacagcggc gatctgcaaa acgtgtactg
    32461 ggatgtgctg gagcgtatta aacgtgaatt tgatgccgcc ggtatcagct tcccgtaccc
    32521 gcaaatggat gtgaacttta agcgggtgaa agaagacaaa gctgcgtaat caacgctgag
    32581 gccagataat actctctggc ctctctctta ttagttattc tgattgccaa ttaatattat
    32641 caatttccgc taataacaat cccgcgatat agtctctgca tcagatactt aattcggaat
    32701 atccaacgtg ttttcttatt actttcaagg tcttgctctt ggggcagcta tgattctgcc
    32761 gctcggccca caaaatgctt ttgtgatgaa tcagggcatt cgtcgtcagt accacattat
    32821 gattgcctta ctttgcgcta tcagcgattt ggtcttgatt tgcgcgggga tttttggtgg
    32881 tagcgcgtta ttgatgcagt cgccgtggtt gctggcgctg gtcacctggg gcggcgtagt
    32941 cttcttgctg tggtatggtt ttggcgcttt taaaacagcg atgagtagta atattgagtt
    33001 agctagtgcc gaagtcctga agcaaggcag atggaaaatt atcgccacca tgttggcagt
    33061 gacctggctg aatccgcatg tttacctgga tacttttgtt gtactgggca gccttggcgg
    33121 gcagcttgac gtcgaaccaa aacgttggtt tgcgctcggg acaattagcg cctctttcct
    33181 gtggttcttt ggtctggcta ttctcgcagc ctggctggca ccgcgcctgc gcacggcaaa
    33241 atcacagcgc attatcaatc tggtagtggg atgtgtcatg tggtttatag ccttgcagct
    33301 ggcgagagac ggtattgctc atgcacaagc cttgttcagt taggcgctat ctgatggaaa
    33361 aataaaacag aggcgctaag cttgcctcca gaggtcctga attttgggca agtcaattaa
    33421 cacggagaga ctaacgtgaa gttcaaagtt atcgccctgg cggcattaat gggtattagc
    33481 gggatggcag cgcaggctaa cgaattgccg gatggaccgc atattgtcac ctccggtacg
    33541 gcaagtgtgg atgcggtgcc agacattgcc actcttgcga ttgaagttaa cgtggccgcg
    33601 aaggatgccg ctacagccaa gaaacaggca gatgagcgcg tcgcacaata catttccttc
    33661 cttgaactca atcagatcgc gaaaaaagat atcagctcag cgaatttacg cacccagcca
    33721 gattatgatt atcaggatgg taaaagtatc ctgaaaggct accgcgctgt gagaacggtg
    33781 gaagtcacgc tccgtcagtt agacaaactg aattccttgc tggatggcgc gctgaaggcg
    33841 ggtcttaacg aaattcgttc tgtgtcgctg ggcgtggcgc agccggatgc ctataaagac
    33901 aaagcgcgta aggcagcgat tgataacgcg attcatcagg cgcaggaact ggcgaacggc
    33961 tttcatcgta aactggggcc ggtatatagc gtgcgctacc atgtttccaa ctatcagccc
    34021 agcccaatag tgcggatgat gaaagccgat gccgcgccgg tgtctgccca ggaaacttac
    34081 gagcaggccg ctattcagtt tgatgatcag gtcgatgtgg tcttccagtt agaacctgtg
    34141 gatcaacaac ccgctaaaac acctgcagca caataatact tgtagcccga cgtattcgtc
    34201 gggctaatgc ctttacccga tattgcctcc tttttctgta attttctgta aaaccatgac
    34261 gtaatcacct atctttgctc gcggtttcat caaggatagg ctgactgaaa cggttaagcg
    34321 gacaaggatg cggggagggg ggatggacat ttttatttcg aaaaagatgc gcaatttcat
    34381 tttattagcg caaaccaata atattgcccg ggcggcagaa aaaatccata tgacggcttc
    34441 gccatttggt aaaagcattg ccgcgctgga agaacaaatt ggctatacgc tatttacccg
    34501 caaagataat aatatcagcc tcaataaagc cggtcaggaa ctgtatcaaa aactgttccc
    34561 ggtttatcaa cgactttctg cgattgataa tgaaatccat aactcagggc gtcgttcacg
    34621 agaaattgtg attggcatag ataatactta tccgacaatt attttcgatc agttgattag
    34681 tctcggagat aagtatgaag gcgtgaccgc tcagccagtt gagttcagcg aaaacggcgt
    34741 tatcgataat ctttttgacc gtcagttaga ttttattatc tccccacagc atgtatctgc
    34801 acgagttcag gaactggaaa acctgactat cagtgaatta ccaccgctgc gattgggctt
    34861 tctcgtttct cgccgttatg aagagcgtca agagcaagag ctgcttcagg agctgccctg
    34921 gctacaaatg cgctttcaaa accgggctaa cttcgaagca atgattgatg cgaatatgcg
    34981 accttgtggg atcaatccca ccattattta tcgtccctac agttttatgg ccaaaatcag
    35041 cgccgtggag cgcggacatt ttctgacggt gatcccgcat tttgcctggc gactggtgaa
    35101 tccggcaacg ctgaaatatt ttgatgcgcc gcacaggccg atgtatatgc aggaatatct
    35161 ttattcaatc agaaatcatc gttataccgc cacgatgctt cagcatattg ttgaagatcg
    35221 tgacgggaca aaccattaac ccagcatcga gccggttgca attaaattac ggtgtaagtc
    35281 gaagacgtgg ctaagatcgt ggtgaatatg tccgccgggc gcattttcca gatagcgatg
    35341 cagataatcc cgatacatag gatgtgcaca attatcaatg atagtgcggg cgcgttgaag
    35401 cggggaaaga ccgcgcagat cggcgatccc ttgttcggtg atgatcactt tgacgctgtg
    35461 ttcgctgtga tcaacatggc tgcacattgg cacgacggtt gagatcttgc cttctttagc
    35521 aatcgacgga gccataaaga tcgacagata cgcgttgcgt tcaaaatcac cgctgccgcc
    35581 gatgccgttc atcagatcga ccccggctac gtgcgttgaa tttgcatgcc cgtaaatatc
    35641 aaactccagg ccgacgttca gagcgatgac gcccagacga cggatgattt ccgggttatt
    35701 ggaaatctcc tgcggacgca acacaatgcg gctggcaaag taatccatat tgtcgtaaat
    35761 cttgcgcagg gaatcggccg agattgtcag gctggaggcg ctggccccgc tgattttgcc
    35821 ggtttccagt aaatgcacca ccgattcctg tagcacttcc gaatacatca tgaacggagg
    35881 aatttccggg ttttccccca gacgcgccat taccgcatta ttgatattgc ccacgccact
    35941 ttgcagcggc agaaattccg gcggaatacg cccatgcgcc atttcctgca ataagaacgt
    36001 gaccacgtta tcggcaatct gctggcacat gggattttgc ttatccagca tattaccggc
    36061 gtcgggcaag ttggtttcca cgacggcgac aatctttttc ggatcgattt gcacatagcg
    36121 ggtaccgacg cgatccattg catggaagat cgacacgcta ttgcgccgcg gtggcgcgcc
    36181 aggaatcaca atatctgcca gttctgcaac gcgcggatcg tgatagtgat taagttcaat
    36241 gatcactttc ttcgctcgca gcagccaggt cggcgcatta ccgatcccgc tggttaacca
    36301 gactcgacca tccggtgcca gtgccgatgc ttcaatgacg gcaacatcaa tgtcgccgaa
    36361 gaaaccgtaa ttgaccattt gcgccacttc gctcaaatgc aggtcaacga aactcaccgc
    36421 gccctgattg atctttttac gtaaaccgga cgatgtttga tatggcgcac gccaggaaac
    36481 agcatcggcg tcagaaagta catcgtcagc ggcggcgctg attgacgccc ccgtcagcag
    36541 gcgaatttga tacggctttt tggcctcatg ctgttcgtta gctctgcggg caatcgcggt
    36601 gggtagggct ttcggcgaac ccgccggggt aaagccgcta aatgccacca tgtcgttatg
    36661 ctggataatt tctgccgctt catcggcggt catccttgtc cactgagttt ccatttttac
    36721 tccttcgttc gcagggatta atgaccaacg aaattaggtt tacgtttttc gaggaaagcg
    36781 ttcatccctt cctggtaatc ttcgctgtca taaaccgcgc ggcgcatccc ctgaatacgt
    36841 tcaaattcat cggagttcat ggtgtgcgct tcgcccagta cacgcagctc ttctttgata
    36901 acagcaatgg ctaacggcgc tttctcagag atgtggtgcg ccatttgtaa ggtgaaatct
    36961 tccagttctt ccacttccac aacatggttg aggatgccga cagccaacgc gcgctgggcg
    37021 gtgattggcg aagcggtaaa aatcagctct ttgacaatgt ggaagcccgc gtcgcgggtc
    37081 aggttgtgaa tgccgaccag gttatacggg acgccgaggt ttacaggcgt cattgagaag
    37141 gttgaggtac tggcggcgat gatcagatcg gaactcatga tcatttcaaa tgcgccaccc
    37201 caaacactac cttccaccat cgaaatgatc ggtttcggga atttttggat catgcgggtg
    37261 atttgacgca atggatcatc ataggagagc ggatcgcgac cgccagacgg cagttcgtga
    37321 atatcgtgac ctgcggagaa gactttggat ccacttggcg cccgcaaaat gatacagcga
    37381 atttccggcc ggttgagatc gcttaacgcc tgcataagat catcaataaa gactttactt
    37441 aaggcattaa gttttcgtcc atagttaaac tcaatgaccg ccactttgtt gatagtggca
    37501 acgttcacat actgataaga cataaaaatt cctttaatca aaatattgcg tctggataaa
    37561 ttcactgagc tgccgcaggc cggtgcgcgg tgagagcgta ttgtttttga ccgctaaaag
    37621 cgtctggcgg taatagcgat cgaaatcttc attcgcgaac aggtgattca gtacttcttc
    37681 ttcggtctgc ttacgcagcc attccaccga ttgttgttgc cgcacttgtt gtaaacgacc
    37741 actggcagtt agcgcggttt tgaagtcgat gatggcgtgc cagatctcat cgattccacg
    37801 tttttccagt gcgctacaag tcagaacccg tggctgccat tcgtcgtatt tacgtcgcag
    37861 aatatgcagg gcactctcgt acatatgccg ggcaatggcg acattggtat ggttatcgcc
    37921 atcgtctttg ttgataacga tcagatcagc cacttccatc agcccttttt taatgccctg
    37981 cagatcatcg ccgccaccgg caatttgcaa cgagataaaa cagtccacca tgcgggcgac
    38041 ttctgtttcc gactgcccga cgccaaccgt ttcgacaatc actacgtcat aacccgctgc
    38101 ttcgcataac agcattaatt cccgcgctcg ctgactggca ccgcccagat gaccggagga
    38161 tggtaccggg cgaataaacg ccgcttcggc acgcgccagg tcattcatgc gggttttatc
    38221 cccgagaatg ctaccgccag tgaccgggct gctgggatcg accgcaataa ccgcgacctt
    38281 taatccctct cgaatcaaca acatgccaaa ggcctcaaga aaggtacttt tccccgcgcc
    38341 gggggggccg gtaacgccca gtcgcagggt gttaccgcag tacggcataa tggcatcaag
    38401 cagctgcgta cttagtgcct gatgacgcgg gtgacggctt tccaccagcg tcatggcctg
    38461 ggcgagtgtg gcacgctcac cctgacgtaa gcggcgaata ctttctgcca gcgtggcttc
    38521 attaatcatg atgctggctt atcagattca gtacgtcgcg cacactgtcg agcataggtg
    38581 tacctggacc ataaatcgcc gccacgccgc gctcttgcag gaaggcgtaa tcctgcggcg
    38641 gaatgacgcc acccgcgacc acgcagatat cttcgcgtcc ccattttttc agcgcttcga
    38701 ccagttccgg gatcagcgtt ttatgaccgg cagccagtga ggatgcgccc actacgtgaa
    38761 cgtcgttttc tacggccagg cgggcgatct cttcaggtgt agagaacatc gggcttaaat
    38821 ctacgtcgaa accgagatcg gaataggcgc tggcgatcac tttcgcgccg cgatcgtgtc
    38881 catcctggcc catcttagcg atcagaatgc gcgggcgacg accattgtcg gcaaggaact
    38941 gctccgtttg cgcaacaatg gcatcgaact cggaggccga tttctcagac tgatgatagc
    39001 tttgcgcaat cacgccggta acacactggc ttggcaccag ataacggtcg aaagcgactt
    39061 ccagcgcatc ggaaatttca cccagggtgg cgcgaacgcg agcggcatta acagcggcag
    39121 ccagcaggtt ttcgttatgc tgtgcggcgt gagtcagggc gttcaacgcg gcggttacgg
    39181 cggcatcatc acgggtggcg cgaatgcgtt ccagcgaagc aatttgctcg ttacgcacca
    39241 tcacgttgtc gatctcaagt acatcggttt cgtcttcgtg ctccagtttg tacttgttga
    39301 caccaacgat gacacgcttg ccctggtcga tcagcgactg ttcacgcgct gaggcctctt
    39361 cgatcattcg ttttggcaga cctgcttcga tcgctttcgc catgccaccg gcttcgtcga
    39421 tctgttggat aatagctctg gcttgtttga cgatttgatc ggtcagcgac tcaatgtaat
    39481 aggatccggc cagtggatcg acggtgcggc agagttctga ttcttcctgg atgatgatct
    39541 gggtgttgcg ggcaatgcgt gctgagaaat cggtaggcaa accaagcgct tcgtcaaagg
    39601 cgttggtatg cagtgactga gtaccgccca gcgtcgcagc cagcgcttca atggtggtgc
    39661 ggataacgtt gttatacgga tcctgttcag tcaggctcca gcctgaggtc tggcagtggg
    39721 tacgcagcgc cagtgatttc gggtcctgtg cgccaaatcc actgaccgct tcgctccata
    39781 aataacgtgc cgcacgcaac atggcgacgt tcataaacag atccatgccg atgccgaaga
    39841 agaacgacag gcgaggagcg aagtcatcaa ttttcagtcc ggcagagatt gctgctttga
    39901 tgtactcaat cccatcagcg agcgtaaatg ctacctgctg cacgcagttg gcacccgctt
    39961 cacccatgtg gtaaccgctg atactgatgg tattaaatcg cggcatgttg ccggaacacc
    40021 aggcgatgat gtcggcgata atgcgcattg acggttttgg cgggtaaata taggtgttgc
    40081 ggcagaggta ctctttgagg atatcgttct gaatggtgcc ggtcagttta tcagttgtaa
    40141 caccttgctc ttgcgcggcg acgatataaa acgccagtac cggcaacacc gcaccgttca
    40201 tggtcatcga aaccgacatt ttatccagcg ggatttgatc gaacagcact ttcatatctt
    40261 ccacggtgtc gatagcgacg ccctctttgc cgacgtcgcc cgccacgcgc gggttatcgg
    40321 agtcgtagcc tcggtgggtg gcaaggtcaa acgcaacgga aagacctttt tgcccggcgg
    40381 ccaggttacg gcgataaaaa gcgttggact cttttgctgt tgaaaaacca gcatactgac
    40441 ggatggtcca cagttgggcg gtatacatag tggcacgcgg gccacgaacg tagggcggca
    40501 aaccaggaag ggtacctgtc acctccagat tatcgagatc ggcttcggta tacagcggct
    40561 tgatggcgat cccttccgcg gtttgctgaa ccagcgagtc gacagttttc tcccgacggc
    40621 tcaattcctt gttggcaagc tgttgccact cctgcacgtt agacattatt tgctccgtaa
    40681 aacgcaatca aaatgagggt atttgcgcct acggtgagct ttttacgtag gtttgtcgcc
    40741 agcaaaaaaa atgcattcag acgtcgtttt ttgccgcctg agatttgtct attttgtgat
    40801 gtgattcaag gaatacaaaa agaagtgagg acaacgcctg atatgtgcta tcaggcatta
    40861 tttgatggat taatcctgac gaaggacttt gtgaccataa tcgagcaacg catcagtgac
    40921 tttacgcatc atgcggcttt caggagcaaa gcggtgccag tagagcatcc ggcgttgaaa
    40981 tagccctggc gttaagtcaa tcagttcacc gctggctagt tctttctcga tttgcaggtg
    41041 cgggatcata cagcaggtgg tgccctggcg agcaagttgc acgaacgctt ctgaagaatt
    41101 aacgatatgg cagggcacgc tgcctggagg cagatcgaag ttttgctgca aaaaggcctg
    41161 gtgcatatcg tcaagatggt caaaggcgac cacaggtgct ttcaataatg ccgaacgcgt
    41221 tacgccgtta gggaaatatt tttcggcaaa cggttttgag ctgacgaaca gatagtcaag
    41281 cgcaccgagc ttatcgacaa ggcaactcgg cagcgcctga tgttgaatac tcaccgcgcc
    41341 gaccacttcg ccgcggcgca gacgttcctg agtacgggtt tcatcttcta cctgcaagtt
    41401 gaggcggata ggcgaatcag ccaacacagg agccagtgcc ggaagcaacc acgtcgccag
    41461 actgtcggcg ttgaccgcca gtgaaagcag cagcggagtc gaaccggttt gttcatcgcc
    41521 cagccactct tcttccagca actccacctg gcgcagcagt gccagcagtt tttgcccttg
    41581 ttccgtcggg cgcggcggta cggtacgcac caacagcggc tgcccgaaca tattttccag
    41641 ttgcttaatg cgctgtgaga cggctgattg tgtaatgcac agcttttgtg ccgcgcgctc
    41701 aaatcctcgt tcacgtatca ccgcatccag tgcctgtaat gttctgtagt ccgggcgttt
    41761 cattgctgtt gtctgctcct ggtaaatgct ttttcctgca ctatgacaca attttgtgtc
    41821 aggttgcata taccgcagcc gtcggcgggt aacatttgta tcaatggtta aacgcgcagc
    41881 ttaaaagatc agggatttgc gctattgcat ttcgcttagc gacttcgcgc cctggttatg
    41941 ttgaatggcg tggcgttatt gcctctgttc gcaccgtgga gcggcatacc cagaccgttg
    42001 tatgctttat ttgcggggcg aaaggggatg cccgccattg cgcgatattt tatggatgag
    42061 ttaaccacgc ggcttgccaa cggggtctga atcgcttttt ttgtatataa tgcgtgtgaa
    42121 atttcatacc acaggcgaaa cgatcatgac gcaggatgaa ttgaaaaaag cagtaggatg
    42181 ggcggcactt cagtatgttc agcccggcac cattgttggt gtaggtacag gttccaccgc
    42241 cgcacacttt attgacgcgc tcggtacaat gaaaggccag attgaagggg ccgtttccag
    42301 ttcagatgct tccactgaaa aactgaaaag cctcggcatt cacgtttttg atctcaacga
    42361 agtcgacagc cttggcatct acgttgatgg cgcagatgaa atcaacggcc acatgcaaat
    42421 gatcaaaggc ggcggcgcgg cgctgacccg tgaaaaaatc attgcttcgg ttgcagaaaa
    42481 atttatctgt attgcagacg cttccaagca ggttgatatt ctgggtaaat tcccgctgcc
    42541 agtagaagtt atcccgatgg cacgtagtgc agtggcgcgc cagctggtga aactgggcgg
    42601 tcgtccggaa taccgtcagg gcgtggtgac cgataatggc aacgtgatcc tcgacgtcca
    42661 cggcatggaa atccttgacc cgatagcgat ggaaaacgcc ataaatgcga ttcctggcgt
    42721 ggtgactgtt ggcttgtttg ctaaccgtgg cgcggacgtt gcgctgattg gcacacctga
    42781 cggtgtcaaa accattgtga aatgatctga cgggggaacc tcccccgtta aaaaaattct
    42841 cttcattaaa tttggtgaca tgtgtcacgc ttttaccagg caattgtcga ttgctctaaa
    42901 taaatcctct aaaccagcat attcaaccaa gaaatacctt tgcgtgatat ttcctcaaca
    42961 tcgcgacgca aacgttcata ttgccgcaat attatttttt gatatgttga aaggcggatg
    43021 caaatccgca cacaacattt caaaagacag gattgggtaa atggcaaagg tatcgctgga
    43081 gaaagacaag attaagtttc tgctggtaga aggcgtgcac caaaaggcgc tggaaagcct
    43141 tcgtgcagct ggttacacca acatcgaatt tcacaaaggc gcgctggatg atgaacaatt
    43201 aaaagaatcc atccgcgatg cccacttcat cggcctgcga tcccgtaccc atctgactga
    43261 agacgtgatc aacgccgcag aaaaactggt cgctattggc tgtttctgta tcggaacaaa
    43321 ccaggttgat ctggatgcgg cggcaaagcg cgggatcccg gtatttaacg caccgttctc
    43381 aaatacgcgc tctgttgcgg agctggtgat tggcgaactg ctgctgctat tgcgcggcgt
    43441 gccggaagcc aatgctaaag cgcaccgtgg cgtgtggaac aaactggcgg cgggttcttt
    43501 tgaagcgcgc ggcaaaaagc tgggtatcat cggctacggt catattggta cgcaattggg
    43561 cattctggct gaatcgctgg gaatgtatgt ttacttttat gatattgaaa acaaactgcc
    43621 gctgggcaac gccactcagg tacagcatct ttctgacctg ctgaatatga gcgatgtggt
    43681 gagtctgcat gtaccagaga atccgtccac caaaaatatg atgggcgcga aagagatttc
    43741 gctaatgaag cccggctcgc tgctgattaa tgcctcgcgc ggtactgtgg tggatattcc
    43801 ggcgctgtgt gatgcgctgg cgagcaaaca tctggcgggg gcggcaatcg acgtattccc
    43861 gacggaaccg gcgaccaata gcgatccatt tacctctcca ctgtgtgaat tcgacaacgt
    43921 ccttctgacg ccacacattg gcggttcgac tcaggaagcg caggagaata tcggcctgga
    43981 agttgcgggt aaattgatca agtattctga caatggctca acgctctctg cggtgaactt
    44041 cccggaagtc tcgctgccac tgcatggtgg tcgtcgtctg atgcacatcc acgaaaaccg
    44101 tccgggcgtg ctaactgcgc tgaacaaaat cttcgccgag cagggcgtca acatcgccgc
    44161 gcaatatcta caaacttccg cccagatggg ttatgtagtt attgatattg aagctgacga
    44221 agacgttgcc gaaaaagcgc tgcaggcaat gaaagctatt ccgggtacca ttcgcgcccg
    44281 tctgctgtac taattcccct tctctgaaaa ttaacgggca ggtcactgac ttgcccgttt
    44341 ttttatccct tctccacacc gttgccgcgt tgcgcattct actttgcgag tctgctccag
    44401 aaaataaatc aaactcagat caatgcgttg tgtttattta atggggattt acgatggcgg
    44461 gacagcatgg ggctgtaacg gtaaagccct caccgaagcg agggcttaaa ggagaagggt
    44521 tatgatgcga cttgtcatca tactgattgt actgttactg ataagtttcg gagcttatta
    44581 acagtcagtc tcaggggagg agtcatcctc ccttaccctt actcactaaa ttaggtcaaa
    44641 gaatcaacga tgtcaatcag ggcgatgcgg ggtgcatcgc cctgaaccga actttttgat
    44701 agccacttca tcgcccacgt ttactgccgt aatagatcct ttttcccaaa aattatctct
    44761 gttttgcatg gggtttttcg agcacgctcg aagaaaagat ttaaaggtag atcaatgcgt
    44821 tgtggttatt taaagagagc ttacgatagc tgggcagaat ggggctgtaa cggtaaagcc
    44881 ctcaccgaag cgagggcttg aaggagaagg gttatgatgc gacttgccat catactgatt
    44941 gtactgttac tcataagttt cccagcttat taacagtcag tcacagggga ggagcaatcc
    45001 tcccttaccc ttactcacta aattaggtta aagaatcaac gatgtcaatc agggcgatgc
    45061 aggtgtatcg cccttaccac tcccagactt tcgacggtgt aaccaccgca ggaagaggga
    45121 tatcccactc ttcaacgggg agtttttcca ccaactgaca atcatgcgca taacccaccg
    45181 gttgcgtttt atagtgctgc cagttttgta aggtccgatc ataaaaacca ccgcccattc
    45241 ccaggcgctg accgtactca tcaaaggcga ccagcggtgt gatcagcacg tctaatcggg
    45301 aaaggggtag cacgtcacgc acatccagtt ttggctcatg gatcttcaac ctgttcatca
    45361 ccagttcgct ttgcggatgg taattcagga acagcaaatt accggcacta aagggatgca
    45421 aaactggaag atatacgcgc ttaccggcgc gccagagttg ttctattagc ggctgggtgt
    45481 cgagttcacc atcaaaagag aggaatacag cgaccgtatg cgccatcacc accgggggat
    45541 aagtcatcat ccgggtagcg gcttgttgac ccatttcctg ctgttgttcc ggcgttaacg
    45601 cacgacgacg ttgccgaatc attttgcgga tttcttgtcg ggataatgtc agtgggagtt
    45661 ctggtagttg cgtcatggct ggtgccagat aagaagggaa tctccgagat gccgccgcag
    45721 gctgtaaccc ttgaaccctt ggttcaaggt gaatgtgtcg tcgcagtttt aaggcttctc
    45781 ggacggaccg agcatgctca ccaaccgcgg agcgccacat tcttgtggta tgaaatatcg
    45841 gctcagggga ctggcccgct tgcgaacatc tcagagaaat tttgtcttca cggttactct
    45901 accacagtaa accgaaaagt gtcattcaaa gttttggtta gttttttcgg tgatgcgacc
    45961 ttgttcaagt aacgcttgtt ctatggtctg ctgcagcatc cgaatacgct gttccatact
    46021 tgccgcgtag tcacgagtct tcgctttttc ttgcgctaac tcatagctga tattcaatgc
    46081 ggcaatgaag accaactgtt cagtatttgt gactctagtg cgttctttca gatcttgcaa
    46141 ccgttggttc agatcgtccg ctgcctgatt caacgcatcc ctttggtcag gcgggcagtt
    46201 cacacgcagt gaacggccaa aaatttggat atcgacgggt tgtgcagaca tgccaccttc
    46261 ctgctgattg actgcgctgc cttcgtcttt agaccccatg gtctgcgaag gggcgacact
    46321 atagctaccc tgatgagaag agacaagccc ttttctggtc caccagggcc caaagtggta
    46381 gcatatcatg aatattcctc cctttgacga cgaatgctta tgtctataca gaacgaaatg
    46441 cctggttaca acgaaatgaa ccagtatctg aaccaacaag ggacgggtct gacccctgcc
    46501 gagatgcatg gtttaatcag tgggatgata tgtggcggta acgatgacag ctcatggctg
    46561 ccgctacttc acgacctgac gaacgaaggc atggctttcg gtcatgagct ggcacaggca
    46621 ctgcgtaaaa tgcactctgc caccagcgat gccctgcagg atgacggctt cctttttcag
    46681 ctttatctgc ctgatggcga tgatgtcagc gttttcgatc gggctgatgc attggcaggt
    46741 tgggtcaatc acttcctgct tggtcttggc gttacgcaac cgaagctgga taaagtgacc
    46801 ggcgaaaccg gtgaagctat cgacgatctg cgtaacattg cgcaactggg ttacgacgaa
    46861 gacgaagatc aggaagagct tgaaatgtcg cttgaagaga tcatcgaata cgttcgtgtt
    46921 gccgcgctgt tatgccacga cacctttact catccgcaac cgaccgcgcc agaagtacaa
    46981 aaaccgactc tacactaaaa acaaaaaacg taaggagagt gttatgagtg agatatcccg
    47041 gcaagagttt cagcgtcgcc gtcaggccct ggtggagcaa atgcaacccg gcagcgccgc
    47101 gctgattttt gctgcaccag aagtaacacg tagcgccgac agcgaatacc cctatcgtca
    47161 gaacagtgac ttctggtact tcaccggctt taacgaaccg gaagcggtgc tggtgctgat
    47221 taaaagcgat gacactcata accacagcgt tctgtttaac cgcgttcgcg acctgacggc
    47281 ggagatctgg tttggccgtc gcttaggcca ggatgccgcg ccagagaaac tgggcgttga
    47341 ccgcgcactg gcattcagcg aaatcaatca gcaactttat caactactta acggcctgga
    47401 tgtggtttac catgcccagg gcgaatatgc atatgctgat gaaatcatga acagtgcgct
    47461 ggaaaaactg cgtaaaggtt cgcggcaaaa tctcaccgca ccggcaacga tgatcgactg
    47521 gcgtcctgtt gttcatgaaa tgcgcctgtt caaatcgcca gaagagattg ccgtactccg
    47581 ccgcgcggga gaaatcagcg ccatggcaca tacacgggcg atggaaaaat gccgtccggg
    47641 aatgttcgag taccatctgg aaggcgaaat tcaccacgaa tttaaccgcc acggtgcgcg
    47701 ctatccgtcc tataacacca ttgtcggcag cggtgaaaac ggctgcattc tgcactacac
    47761 cgaaaacgag agtgaactgc gcgacggtga cctggtgttg attgacgcgg gttgtgaata
    47821 caaaggttac gcgggcgata ttacccgcac cttcccggtc aacggcaaat tcacccaggc
    47881 ccagcgtgaa atctacgaca ttgtgctgga gtctctcgaa accagcctgt gcctgtatcg
    47941 tccgggaact tccattctgg aagtcactgg tgaagtggtg cgcatcatgg ttagcggcct
    48001 ggtaaaactc ggcatcctga aaggtgatgt tgatgaactg atcgctcaga acgcccatcg
    48061 tcctttcttt atgcatggcc ttagccactg gttaggactg gatgtccatg acgttggcgt
    48121 ttatggtcag gatcgctcgc gcattctgga accggggatg gtactgaccg tagagcctgg
    48181 tctgtatatc gcgccggatg cggatgtgcc agaacaatat cgcggtatcg gcattcgtat
    48241 tgaagacgac attgtgatta ccgaaaccgg caacgaaaac ctcaccgcca gcgtggtgaa
    48301 aaagccggaa gaaatcgaag cgttgatggc agctgcgaga aagcaatgag cgtaatcatc
    48361 gtcggtggcg gcatggcggg cgcgacgctg gcgctggcta tttcccggtt aagtcacggg
    48421 gcgctgccgg tacatttgat tgaagcgacc gcgccagagt cacatgctca tccgggcttt
    48481 gatggacgag ctattgcgct ggctgcgggg acctgtcagc aactggcgcg catcggcgtc
    48541 tggcaatctc tggcggatta cgcaaccgcc atcactaccg tgcatgtcag cgatcgtggt
    48601 cacgctggat ttgtcaccct cgccgcagaa gattaccaac tggcggcgct gggacaggtt
    48661 gtcgaattgc acaatgtcgg gcaacggctg tttgcattgc tgcgtaaagc acctggcgta
    48721 acgctgcatt gccctgatcg cgtggctaac gttgcccgta ctcagagtca cgttgaagtg
    48781 acgctggaga gtggcgagac gctgacgggc cgcgtgctgg tagcagctga tggcacccat
    48841 tcagcgttag ccaccgcgtg cggcgttgac tggcagcagg agccttacga acaactggcc
    48901 gtgattgcca actttgcgac ttccgttgcg cacgaaggcc gcgcttttga acgcttcacg
    48961 caacatggcc cgctggcgat gttgccgatg tctgacggac gctgttcgct ggtctggtgt
    49021 catccactgg aacggcgcga agaggtgctg tcgtggagcg acgagaagtt ttgccgtgaa
    49081 ctccagtcgg cctttggctg gcgacttgga aaaattaccc acgccggtaa acgcagtgct
    49141 tatccgctgg cgttaacccg cgccgccaga gctattaccc atcgtaccgt gctggtgggc
    49201 aatgcggcgc aaactctgca cccgattgcc gggcaagggt ttaacctcgg tatgcgagat
    49261 gtcatgagcc ttgcggaaac cctgactcag gcgcaggagc gcggagaaga catcggtgat
    49321 tacggcatat tgtgccgcta tcagcagcgt cgacagagcg atcgcgaagc aaccatcggc
    49381 gtcacggaca gcctggtaca tctttttgcc aaccgttggg caccgctggt tgtcgggcgc
    49441 aacatcggac tgatgacgat ggaattattc accccggcac gcgatgtgct ggcgcagcgc
    49501 accctcggtt gggtggcgcg ttgaaggcgg ttttaaggaa taaacaatgc aaagtgttga
    49561 tgtagccatt gttggtggcg gcatggtggg gctggcggtt gcctgcggct tacaggggag
    49621 cggcttacgc gttgccgtac tggagcagcg cgtaccggaa cctctggtgg cggatgcacc
    49681 accacaactg cgcgtttcgg ctatcaatgc cgccagcgaa aaattactca cccgtcttgg
    49741 cgtctggcag gacattctct ctcgtagggc cagttgttat cacggtatgg aagtgtggga
    49801 caaagacagt tttggtcaca tttcgtttga cgatcaaagc atgggctata gccatcttgg
    49861 gcatatcgtt gaaaactcag tgattcacta cgcgctgtgg aacaaagcgc agcagtcgtc
    49921 agatatcact ctgttggccc ccgcagaatt acagcaggtc gcctggggag aaaatgaaac
    49981 tttcctgacg ctgaaagatg gcagcatgtt aacggcgcgt ctggtgattg gcgcggacgg
    50041 tgctaattcc tggttgcgca acaaagcaga tattccgctg actttctggg attatcagca
    50101 tcacgcgctg gtagcgacca ttcgcacgga agaaccgcat gatgcggtgg cgcggcaggt
    50161 tttccatggc gaaggcattc tggccttttt accgcttagc gatccgcatc tttgctcaat
    50221 tgtctggtca ctgtcgccag aggaagcgca gcggatgcag caggcaagtg aagatgaatt
    50281 taatcgcgcg ttaaatatcg cttttgataa tcgcctgggc ttatgcaagg ttgagagcgc
    50341 gcgtcaggtg ttcccactga cggggcgtta tgcgcgccag tttgccgcgc accgtctggt
    50401 gctggtgggc gacgccgcgc ataccattca cccgctagcg gggcagggag taaatctcgg
    50461 ctttatggat gctgcagagc tgattgctga actgaaacgg ttgcatcgtc aggggaaaga
    50521 catcgggcag tacatttacc tgcgccggta tgagcgtagc cgcaagcaca gtgcggcgct
    50581 gatgctggct ggtatgcagg gattccgcga tctgttttcc ggtaccaatc cggcgaaaaa
    50641 actgctgcgt gatattggtt tgaaactggc cgacacgctt cctggcgtta aaccacaact
    50701 tatccgccag gcaatgggat taaacgattt gcctgaatgg ctgcgctaaa aatttctctt
    50761 ctgctgttta tttgataccc atcacacttt catctcccgg ttttttcgcc gggagatttt
    50821 cctcatttga aataaactaa tttcacctcc gttttcgcat tatattttct aatgtcatta
    50881 ttttttgatt tagtgttttt tgacattttt ttagctctta atattgcctt attcaaatta
    50941 gctttctaat ggcatcatct ttatatagaa actggtgtgt tttttggttt tttattctgt
    51001 cgcgattttg gcgtttttta accataagct aatgtgatga tcaattttac cttatggtta
    51061 acagtctgtt tcggtggtaa gttcaggcaa aagagaacga ttgcgttggg gaccgggagt
    51121 ggctccgatg ctgggtttcg tggtgataat ttcaccatga aaagttgtca gccccgctta
    51181 ttcaatgagg acaagatggc acaacagact cctttgtacg aacaacacac gctttgcggc
    51241 gctcgcatgg tggatttcca cggctggatg atgccgctgc attacggttc gcaaatcgac
    51301 gaacatcatg cggtacgtac cgatgccgga atgtttgatg tgtcacatat gaccatcgtc
    51361 gatctccgcg gcagccgcac ccgggagttt ctgcgttatc tgctggcgaa cgatgtggcg
    51421 aagctcacca aaagcggcaa agccctttac tcggggatgt tgaatgcctc tggcggtgtg
    51481 atagatgacc tcatcgtcta ctactttact gaagatttct tccgcctcgt tgttaactcc
    51541 gccacccgcg aaaaagacct ctcctggatt acccaacacg ctgaaccttt cggcatcgaa
    51601 attaccgttc gtgatgacct ttccatgatt gccgtgcaag ggccgaatgc gcaggcaaaa
    51661 gctgccacac tgtttaatga cgcccagcgt caggcggtgg aagggatgaa accgttcttt
    51721 ggcgtgcagg cgggcgatct gtttattgcc accactggtt ataccggtga agcgggctat
    51781 gaaattgcgc tgcccaatga aaaagcggcc gatttctggc gtgcgctggt ggaagcgggt
    51841 gttaagccat gtggcttggg cgcgcgtgac acgctgcgtc tggaagcggg catgaatctt
    51901 tatggtcagg agatggacga aactatttct cctttagccg ccaacatggg ctggactatc
    51961 gcctgggaac cggcagatcg tgactttatc ggtcgtgaag ccctggaagt gcagcgtgag
    52021 catggtacag aaaaactggt tggtctggtg atgaccgaaa aaggcgtgct gcgtaatgaa
    52081 ctgccggtac gctttaccga tgcgcagggc aaccagcatg aaggcattat caccagcggt
    52141 actttctccc cgacgctggg ttacagcatt gcgctggcgc gcgtgccgga aggtattggc
    52201 gaaacggcga ttgtgcaaat tcgcaaccgt gaaatgccgg ttaaagtgac aaaacctgtt
    52261 tttgtgcgta acggcaaagc cgtcgcgtga tttacttttt tggagattga ttgatgagca
    52321 acgtaccagc agaactgaaa tacagcaaag aacacgaatg gctgcgtaaa gaagccgacg
    52381 gcacttacac cgttggtatt accgaacatg ctcaggagct gttaggcgat atggtgtttg
    52441 ttgacctgcc ggaagtgggc gcaacggtta gcgcgggcga tgactgcgcg gttgccgaat
    52501 cagtaaaagc ggcgtcagac atttatgcgc cagtaagcgg tgaaatcgtg gcggtaaacg
    52561 acgcactgag cgattccccg gaactggtga acagcgaacc gtatgcaggt ggctggatct
    52621 ttaaaatcaa agccagcgat gaaagcgaac tggaatcact gctggatgcg accgcatacg
    52681 aagcattgtt agaagacgag taacggcttt attcctcttc tgcgggagag gatcagggtg
    52741 aggaaaattt atgcctcacc ctcactctct tcgtaaggag agaggttcac aattcactgc
    52801 acgtttcagg aaccatcgct catgacacag acgttaagcc agcttgaaaa cagcggcgct
    52861 tttattgaac gccatatcgg accggacgcc gcgcaacagc aagaaatgct gaatgccgtt
    52921 ggcgcacaat cgttaaacgc gctgaccggc cagattgtac cgaaagatat tcagcttgcg
    52981 accccgccgc aggttggcgc accggcgacc gaatacgccg cactggcaga actcaaggct
    53041 attgccagtc gcaataaacg cttcacgtct tacatcggca tgggttacac cgccgtgcag
    53101 ctaccgccgg ttatcctgcg taacatgctg gaaaatccgg gctggtatac cgcgtacact
    53161 ccgtatcaac ctgaagtctc ccaaggccgc cttgaagcac tgctcaactt ccagcaggta
    53221 acgctggatt tgactggact ggatatggcc tctgcttctc ttctggacga ggccaccgct
    53281 gccgccgaag caatggcgat ggcgaaacgc gtcagcaaac tgaaaaatgc caaccgcttc
    53341 ttcgtggctt ccgatgtgca tccgcaaacg ctggatgtgg tccgtactcg tgccgaaacc
    53401 tttggttttg aagtgattgt cgatgacgcg caaaaagtgc tcgaccatca ggacgtcttc
    53461 ggcgtgctat tacagcaggt aggcactacc ggtgaaattt acgactacac tgcgcttatt
    53521 agcgaactga aatcacgcaa aattgtggtc agcgttgccg ccgatattat ggcgctggtg
    53581 ctgttaactg cgccgggtaa acagggcgcg gatattgttt ttggttcggc gcaacgcttc
    53641 ggcgtgccga tgggctacgg tggcccacac gcggcattct ttgcggcgaa agatgaatac
    53701 aaacgctcaa tgccgggccg tattatcggt gtatcgaaag atgcagctgg caataccgcg
    53761 ctgcgcatgg cgatgcagac tcgcgagcaa catattcgtc gtgagaaagc gaactccaac
    53821 atttgtactt cccaggtact gctggcaaac atcgccagcc tgtatgccgt ttatcacggc
    53881 ccggttggcc tgaaacgtat cgctaaccgc attcaccgtt tgaccgatat cctggcggca
    53941 ggcctgcaac aaaaaggtct gaagctgcgc catgcgcact atttcgacac tctgtgtgtg
    54001 gaagtggccg acaaggcggg cgtactggcg cgtgccgaag cggctgaaat caacctgcgt
    54061 agcgatatcc tgaacgcggt tgggatcacc cttgatgaaa ccaccacgcg tgaaaacgta
    54121 atgcagcttt tcagcgtgct gctgggcgat aaccacgggc tggagatcga cacgctggac
    54181 aaagacgtgg ctcacgacag ccgctctatt cagcctgcga tgctgcgcga cgacgaaatc
    54241 ctcactcatc cggtgtttaa tcgctaccac agcgaaaccg aaatgatgcg ctatatgcac
    54301 tcgctggagc gtaaagatct ggcgctgaat caggcgatga tcccgctggg ttcctgcacc
    54361 atgaaactga acgccgccgc cgagatgatc ccaatcacct ggccggaatt tgccgaactg
    54421 cacccgtttt gtccgccgga acaggctgaa ggttatcagc agatgattgc gcagctggct
    54481 gactggctgg tgaaactgac cggttacgac gccgtttgta tgcagccgaa ctccggcgca
    54541 cagggcgaat acgcgggcct gctggcgatt cgtcattatc atgaaagccg caacgaaggg
    54601 catcgcgata tctgcctgat cccggcttct gcgcacggaa ctaaccccgc ttctgcacat
    54661 atggcaggaa tgcaggtggt ggttgtggcg tgtgataaaa acggcaacat cgatctgact
    54721 gatctgcgcg cgaaagcgga acaggcgggc gataacctct cctgtatcat ggtgacttat
    54781 ccttctaccc acggcgtgta tgaagaaacg atccgtgaag tgtgtgaagt cgtgcatcag
    54841 ttcggcggtc aggtttacct tgatggcgcg aacatgaacg cccaggttgg catcacctcg
    54901 ccgggcttta ttggcgcgga cgtttcgcac ctcaacctgc ataaaacttt ctgcattccg
    54961 cacggcggtg gtggtccggg tatgggaccg atcggtgtga aagcgcatct ggcaccgttt
    55021 gtaccgggtc atagcgtggt gcaaatcgaa ggcatgttaa cccgtcaggg cgcagtttct
    55081 gcggcaccgt tcggtagcgc ctctatcctg ccaatcagct ggatgtacat ccgcatgatg
    55141 ggcgcagaag ggctgaaaaa agcaagccag gtggcaatcc tcaacgccaa ctatattgcc
    55201 agccgcctgc aggatgcctt cccggtgctg tataccggtc gcgacggtcg cgtggcgcac
    55261 gaatgtattc tcgatattcg cccgctgaaa gaagaaaccg gcatcagcga gctggatatt
    55321 gccaagcgcc tgatcgacta cggtttccac gcgccgacga tgtcgttccc ggtggcgggt
    55381 acgctgatgg ttgaaccgac tgaatctgaa agcaaagtgg aactggatcg ctttatcgac
    55441 gcgatgctgg ctatccgcgc agaaattgac caggtgaaag ccggtgtctg gccgctggaa
    55501 gataacccgc tggtgaacgc gccgcacatt cagagcgaac tggtcgccga gtgggcgcat
    55561 ccgtacagcc gtgaagttgc ggtattcccg gcaggcgtgg cagacaaata ctggccgacg
    55621 gtgaaacgtc tggatgatgt ttacggcgac cgtaacctgt tctgctcctg cgtaccgatt
    55681 agcgaatacc agtaattcac tgattcgact attttctaaa ggcgcttcgg cgccttttta
    55741 gtcagatgac aaagtacaaa agtgctcaga cagtcccctc gcccctttgg ggagagggtt
    55801 agggtgaggg gaacaggccg gcactggcgc gaatatttac cctcaccccg gccctctccc
    55861 tgaaagggcg aggggaaaag cgagccaaca ttgaagattg agccagtttg ttagcaatct
    55921 caaagatacg tcaacgaatt aatttttctc ggaaaaacaa atggctatag cacttgtgac
    55981 cggtggcagt cgcggcatcg ggcgggcaac tgcattactg ttggcgaaag aagagtatac
    56041 ggtggcggtt aattatcaac aaaacctcca tgcggcgcag gaagtggtga acttaatcac
    56101 gcaagccggt ggcaaggcat tcgtgctcca ggcggatatc agcgacgaaa atcaggtcat
    56161 cgcaatgttt acagcaatcg atcagcacga tgaaccgcta gcagcgctgg tcaataacgc
    56221 cgggatcttg tttacccagt gcaccgttga aaaccttacc gcagagcgaa tcaaccgagt
    56281 actttccacc aacgtgacgg gatattttct ctgctgccgc gaggcggtaa aacgcatggc
    56341 gcttaaaaat ggtggcagtg gcggcgctat cgtcaatgtc tcttcggtgg cctcacggtt
    56401 gggttcgcca ggggaatatg ttgattacgc ggcatcgaaa ggggcgattg atacgttaac
    56461 caccggacta tcgctggaag tcgccgcgca ggggatccgc gttaactgcg tgcggccagg
    56521 gtttatttat accgaaatgc acgccagcgg cggcgagcct ggacgcgtcg atcgcgttaa
    56581 gtcgaacatc cccatgcagc gtggtggaca ggcagaagag gtcgcgcagg ccattgtctg
    56641 gctactaagt gataaagcct cttacgtcac gggaagtttt atcgatttgg cgggcgggaa
    56701 ataaaacagg gaagttgtct gaccggatgc aacaagtatt gcatccggta cttcatcgac
    56761 ttaaagcttc tcgccgttgc tggcaatcac ctctttgtac cagttaaagc tcttcttacg
    56821 tgaacgcgac atatcgccag taccgtcgtc atgtttattc acatagataa agccgtagcg
    56881 tttgctgtac tgcccggtag tgaatgacac gcagtcgatg cagccccacg gtgtgtagcc
    56941 catcagatcc acaccatcgt aagtcactgc ttttttcatc tcttcaatat gggcgcgcag
    57001 gtagtcaatg cggtagtcgt cgttgatgct gccatcttct tccactttgt cgtaagcgcc
    57061 aaaaccgttt tcgacaataa acagcggcct ctgataacgc tcatacagtt cgcaaagggc
    57121 atagcgcaga cctactggat caatctgcca gccccagtca gacgctttaa catacgggtt
    57181 cggtacgctg ccttcaaaac cagagatcgc atcgccggtg ccgccttcgg ccttcactgc
    57241 gttggtcatg taatagctga aaccaagata atcgcaggtg ccttcacgca gtacatccag
    57301 atcgccatct tccattttga tgttaaatcc gcgacgctcc cactcgttca acacatagga
    57361 cgggtaatag ccgcgtagct gcacatcggt aaagacgtag cgttcgcgca tcgactcctg
    57421 agcgaacatc acatcgtccg ggttacagga gtaaggatag agcggcacca tcgccagcat
    57481 acagccgact ttcatctccg ggttaatgcg acgcgcggct ttcactgcca gggcgctggc
    57541 gacaaactgg tgatgcagca cctggtacat cgtctcttcc gggttgtcat gctcggtata
    57601 caccacgccg gagcagcagt aaccaaacag cggcgcacgc cagttacgct ggttgtttat
    57661 ttcgttgaag gtcatccagt atttgacttt atgcttgtag cgttcgaata ccacttccgc
    57721 gaaacggaca aataaatcaa cgactttacg gttggtccag ctaccgtatt gctgcaccag
    57781 atgcagcggc atttcaaagt gggagagggt tatcaccggt tcgatgttgt atttcagcag
    57841 ttcatcgaac agagagtcgt aaaacttcag cccttcttcg tttggctgag cttcatcacc
    57901 ttttggaaaa atacgcgtcc aggcgatgga agtccgaaaa catttgaagc ccatttcggc
    57961 aaatagcttg atgtcttcct tgtagtgacc ataaaaatca acggcttcat ggtttggata
    58021 gtattttcct ggcaaaactt ctttggtgat ttcgcgcggc acgccgtgtg cgccaccggt
    58081 cagaacgtca caaatgctcg gtcctttgcc gcctttgttc cagccgcctt cgacctgatg
    58141 agcggcaacg gcgccacccc ataagaaatc tttcggtaag gtgagttttt tcactatcat
    58201 aggctccaga tgagtcaatt cttacaggaa atagtaccat gcagccaaac gacatcactt
    58261 tttttcaacg tttccaggat gacattctgg ctgggcgtaa aaccatcacc atccgcgacg
    58321 agtctgaatc gcacttcaaa acgggtgatg tgcttcgtgt cgggcgtttt gaagatgacg
    58381 gttatttttg cacgattgaa gtcaccgcaa cctcaaccgt aacgctggat acgctgacgg
    58441 aaaaacatgc agagcaggaa aatatgaccc tgactgaact gataaaggtc attgccgaca
    58501 tctatcccgg tcagacacag ttttatgtga ttgaatttaa atgtctttaa attgaactca
    58561 tgcctgttag ttgaaatttc tcatttaatt tcatgatttt taggagatta gtctcaaatc
    58621 agaatttatt ttagctaaca ggtgttcact ggaactattc tcagttacgc tggagaggta
    58681 tacatgtgcg tgtacgtttc tccggagtaa gttatggttc agaagcccct cattaagcag
    58741 ggatattcgc tggcagagga aattgccaac agcgtcagtc acggcattgg gttggtgttt
    58801 ggtatcgttg ggctggtgtt gctactggtt caggcggtgg atctcaatgc cagcgccaca
    58861 gcgataacca gctacagcct ctatggcggc agtatgatcc tgctgttcct cgcttcgacg
    58921 ctctatcacg ccattcctca tcaacgggca aaaatgtggc tgaagaaatt tgaccattgc
    58981 gctatttacc tgttgattgc cggaacctac acgccgtttt tgctggtggg gctggattct
    59041 ccgttagcgc gcgggttgat gattgttatc tggagcctgg cattgctggg tattctgttt
    59101 aaactgacca tcgcgcaccg attcaaaatt ttatctctgg tgacctatct ggcgatgggc
    59161 tggctgtcgc tggtggtaat ttatgaaatg gcagttaagc tcgcggcggg cagcgttacc
    59221 ttattggcgg taggcggcgt ggtttattcg ctcggagtga ttttctatgt ctgcaaacgc
    59281 attccctaca atcatgccat ctggcacggc ttcgtgctcg gcggcagtgt gtgccacttt
    59341 ctggcgatct atttgtatat tgggcaggcg taatgggtat gcctttgtag gcctgaaaag
    59401 acgcgtcagc gtcgcatcag gcatcggagc acaactgccg gattcggcgt gaacgcctta
    59461 tccgatctac gggcctcgtc cctgttgtga taaaggcatc aaatcccaga catgataaga
    59521 cgcgtcagcg tcgcatcagg cgataaatgc ctgatgcgat acagagattt actcttcgag
    59581 cgaatacggc agcggctcga tatgcagtgt attcgcatcg tcgcgtacgc ggaagatgct
    59641 gtccggttcc atatcgttat tcatgacgac ctgtaccacg acctgaccat cttccagttt
    59701 taccgcagcc agcaccgtac cggtacgacg ccagttctcg cccattttca gctctaagtc
    59761 ttcaccagct tccggcagtc ggctggcact acctgccagc aaccagagcg cgcgtttgtt
    59821 ggcaccacgg aattttgctc gcgccaccat ctcttgtccg gtataacagc ctttcttaaa
    59881 gctgataccg cccagcgcct ggagattggt cgcctgtgga ataaactgcc cgctgttggc
    59941 ggcatcaatc accgggaaac cagcttcaat gtttaatgcc agccactgtt ggctattgtt
    60001 cagttccgct tcaccgcgca gtttatcggt cagcatatta gcagtagctt catcggttac
    60061 gatcaggaaa cgttctgccg ggtgttcaaa ccatagcaaa gtggtcgcgc cttctttgac
    60121 tacctgtttt tctttcgaag gcagttcgct aaagagattt gccagcgcgg cgcgcgcctg
    60181 aaaaccggca acaccaagca gcacacgctc gtcgtctggc gcgatggtca ctttagagaa
    60241 taccgcatat tttttcagtt cagtcagctg cggttcacgc acgctgcgcc gttcaatcca
    60301 tgcaaagcca tcgccgtcgc ggaacagacg taaattgctc cacattttac ctttggcgtc
    60361 gcaatgggcg gcgagcaggt gctgatcttc tgccatctgg ctgacatctg ctgtcacctg
    60421 accctgcata tatttttcgc tgtccgcgcc agtaatggtg gcaagcgccc agtcatcaag
    60481 cgtcatcagc gtcagcggta aacgggcaga agccgtaggc tgacgtggag gaaaaggtgt
    60541 aaaagccata atagagtcct gagttgctta acggcgtatt aatgactaat ggtaaaagag
    60601 ctatgaagca atgcaagtgc tttcactacg cgatctgtgc cttctgcgag gcgacttcca
    60661 aaaaaatgaa gccattgaat tgttgtgaat taatttcgcg atcgcgatgg tgtttactga
    60721 tattcccgta aaagaaggcg aaaaacacgt tacaatagcg gggtactgga ttttcgtgag
    60781 acaggaagaa gatggacatt aacaacaaag cccgcattca ttgggcatgc cgccgtggta
    60841 tgcgcgaact cgatatttca atcatgccgt ttttcgaaca tgagtacgac agcttaagcg
    60901 atgacgaaaa acgcatcttt attcgtctgc tggaatgtga cgatccggac ctgtttaact
    60961 ggctgatgaa tcacggtaaa ccggccgatg cagaactgga aatgatggtc cgactcatcc
    61021 agacacggaa ccgggaacgt ggtcctgtgg caatctgatt tgcgcgtctc ctggcgcgca
    61081 cagtggcttt ccttgctgat tcatgggctg gttgccgctg ttattttact catgccctgg
    61141 ccgctcagtt acaccccgtt atggatggtg ttactttcgc tggtggtatt tgattgcgtt
    61201 cgcagccagc ggcgtattaa tgctcgccag ggggaaattc gcttgttgat ggacgggcgt
    61261 ttgcgttggc aagggcagga gtggagcatc gtcaaagcac cgtggatgat taagagcggc
    61321 atgatgctgc gtttacgttc tgatggcggt aaacggcaac atttatggct ggcagccgac
    61381 agcatggacg aagctgaatg gcgggattta cggcggattt tgttgcaaca agagacgcaa
    61441 agataaagat gacggcaaag catatgcgct aagccgtcag tgagatcagg cgtaatgctc
    61501 tgccatttcg ttgaggattt gctcgcacca gctctgaata cgctcgtcgc taaggtcata
    61561 ctggttagtt tcatccagcg ccagacccac gaacagttgc ccgtcagcaa tcaccggttt
    61621 cgggctggta aattcatatc cttccgttgg ccagtagccg acgaacttca cgcctttggt
    61681 cgagagtttg tcatgcagca taccgagcgc atcgaggaac cactcgccgt atcccagttg
    61741 atcgccaagc ccatacagcg caacaatttt accttcaagg ttcaggtcgt cgagctgatc
    61801 ccagacggct tcccagtctt cctggatttc gccaaaatcc caggtcggga tacccagaat
    61861 gagcacatcg tactgctcca ttaatttcgg ggagtcgtcc ttgaggttat gtaaggtcac
    61921 caattctggg ccgataatat cgcggatttt ttctgccgcc atttcggtgt aacaggtgct
    61981 ggaaccgtaa aaaagaccca tattcataac gtaagcttct cgattctgct gtggcttttg
    62041 cagctagtgt atcagaatcg ctttagtcac ggcacaataa ggcataatgg atcaaatgag
    62101 tgaccataag gggcgcaagt gaaacaggat ctggcacgca tcgagcagtt tcttgatgct
    62161 ctgtggctgg aaaaaaatct ggctgaaaat acgttgaacg cttaccgtcg cgatctgtca
    62221 atgatggtgg agtggttgca tcaccgcggg ttgacgctgg cgacggcgca aagtgacgat
    62281 ttgcaggcat tactggcaga acggctggag ggcgggtata aagccaccag ctcagcgcgt
    62341 ttgctgagtg cagtgcgccg attgttccag tatctttatc gcgaaaagtt tcgtgaagac
    62401 gatcccagtg cgcatctcgc ttcaccgaaa ttgccccagc gtttgccaaa agatttaagc
    62461 gaagcgcagg ttgaacgttt attacaggca ccattaattg atcagccact ggagctacgc
    62521 gataaagcca tgcttgaagt gttgtatgct accgggctgc gtgtctctga actggtcggg
    62581 ctgacaatga gtgatatcag cctgcgtcag ggcgtagtgc gggtcattgg taaaggcaac
    62641 aaagagcgcc tggtgccgtt aggtgaagag gcggtttact ggctggaaac gtatctcgag
    62701 catgggcgac cgtggttgtt gaacggtgtc tctattgatg tgttgttccc cagccagcgt
    62761 gcgcagcaga tgacgcgaca gaccttctgg caccgcatta aacattatgc cgtgctggcg
    62821 ggtatcgaca gcgaaaagct gtcaccgcat gtgttgcgtc acgcttttgc cactcattta
    62881 ttaaatcatg gtgcggattt acgcgtggtg cagatgttgt tgggtcatag cgatctctcc
    62941 accacccaaa tttatacgca tgtcgccacc gagcgtttgc gtcaacttca tcaacagcat
    63001 cacccgcggg cttgattgct gagaaaacag gacgatttat gaagaaaggt tttatgttat
    63061 ttacgttgtt ggcggcgttt tcaggctttg ttcaggcgga tgacgcggca attcaacaaa
    63121 cgttagccaa aatgggcatt aaaagcagcg atattcagcc agcacccgtg gctggcatga
    63181 agactgtgct gactaacagc ggtgtgttgt acatcaccga tgacggtaaa catatcattc
    63241 aggggccaat gtatgacgtt agtggcgcgg ctccggtcaa tgtcaccaat aaaatgctgc
    63301 taaagcagtt gaatgcgctt gaaaaagaga tgatcgttta taaagcgccg caggaaaaac
    63361 acgtcatcac cgtgtttact gatattacct gtgggtactg ccacaaactg catgagcaaa
    63421 tagcagacta caacgcgctg gggatcaccg tgcgttatct tgctttcccg cgccaggggc
    63481 tggagagcca ggctgagcaa caaatgaaag ccatctggtg tgcgaaagat aaaaacaaag
    63541 cgtttgatga tgtgatggct ggtaaagctg ccactccggc aagttgcgat atcgatattg
    63601 ccgatcatta cgcacttggc gtccagcttg gcgttagcgg cactccggca attgtgctga
    63661 gcaatggcac acttgttccg ggttaccagc cgccgaaaga gatgaaagaa ttcctcgacg
    63721 aacaccaaaa aatgaccagc ggtaaataat tcgcgtgaaa caacagatac aacttcgtcg
    63781 ccgtgaagtc gatgaaacgg cagacttgcc cgctgaattg cctcccttgc tgcgccgttt
    63841 atacgccagc cggggagtac gcagtgcgca agaactggaa cgcagtgtta aaggtatgtt
    63901 gccctggcag caactaagcg gtgtcgaaaa ggccgttgag atcctttaca acgcttttcg
    63961 cgaaggaacg cggattattg tggtcggtga tttcgacgcc gacggcgcga ccagcacggc
    64021 tttaagcgtg ctggcgatgc gctcgcttgg ttgcagcaat atcgactacc tggtaccaaa
    64081 ccgtttcgaa gacggttacg gcttaagccc ggaagtggtc gatcaggccc atgcccgtgg
    64141 cgcgcagtta attgtcacgg tggataacgg tatttcctcc catgcggggg ttgaacacgc
    64201 tcgctcgttg ggcattccgg ttattgttac cgatcaccat ttgccaggcg aaacattacc
    64261 cgcggcggaa gcgatcatta accctaactt gcgcgactgt aatttcccgt cgaaatcact
    64321 ggcaggcgtg ggtgtggcgt tttatctgat gctggcgctg cgcacctttt tgcgcgatca
    64381 gggctggttt gatgagcgtg gcatcgcaat tcctaacctg gcagaactgc tggatctggt
    64441 agcgctgggg acagtagcgg acgtcgtgcc gctggacgct aataatcgca ttctgacctg
    64501 gcaggggatg agtcgcatcc gtgccggaaa gtgccgtccg gggattaaag cgctgctgga
    64561 agtggcaaat cgcgatgcgc aaaaactcgc cgccagcgat ttaggttttg cgctggggcc
    64621 acgtctcaat gctgccgggc gactggacga tatgtccgtc ggtgtggcgc tgttgctgtg
    64681 cgacaacatc ggcgaagcgc gcgtgctggc aaatgaactc gatgcgctaa accagacgcg
    64741 aaaagagatc gaacaaggaa tgcaagttga agccctgacc ctgtgcgaga aactggagcg
    64801 aagtcgcgac acgctacccg gcgggctggc aatgtatcac cccgaatggc atcagggcgt
    64861 tgtcggtatt ctggcttcgc gcatcaaaga gcgttttcac cgtccggtta tcgcctttgc
    64921 gccagcaggt gatggtacgc tgaaaggttc aggtcgctcc attcaggggc tgcatatgcg
    64981 tgatgcactg gagcgattag acacactcta ccctggcatg atgctgaagt ttggcggtca
    65041 tgcgatggcg gcgggtttgt cgctggaaga ggataaattc gaactctttc aacaacggtt
    65101 tggcgaactg gttactgagt ggctggaccc ttcgctattg caaggcgaag tggtgtcaga
    65161 cggtccgtta agcccggccg aaatgaccat ggaagtgtcg cagctgctgc gcgatgctgg
    65221 cccgtggggg cagatgttcc cggagccgct gtttgacggt catttccgtc tgctgcaaca
    65281 gcggctggtg ggcgaacgtc atttgaaggt gatggtcgaa ccggtcggcg gcggtccact
    65341 gctggatggt attgctttta atgtcgatac cgctctctgg ccggataacg gcgtgcgcga
    65401 agtgcaactg gcttacaagc tcgatatcaa cgagtttcgc ggcaaccgca gcctgcaaat
    65461 tatcatcgac aatatctggc caatttagcg tcatcttctc tataaaaaag agcgtggatt
    65521 gggtacaatc ccgctcttat caccgcattt tgactagctc aataaaagaa atcagaccat
    65581 gtttgaaatt aatccggtaa ataatcgcat tcaggacctc acggaacgct ccgacgttct
    65641 tagggggtat ctttgactac gacgccaaga aagagcgtct ggaagaagta aacgccgagc
    65701 tggaacagcc ggatgtctgg aacgaacccg aacgcgcaca ggcgctgggt aaagagcgtt
    65761 cctccctcga agccgttgtc gacaccctcg accaaatgaa acaggggctg gaagatgttt
    65821 ctggtctgct ggaactggct gtagaagctg acgacgaaga aacctttaac gaagccgttg
    65881 ctgaactcga cgccctggaa gaaaaactgg cgcagcttga gttccgccgt atgttctctg
    65941 gcgaatatga cagcgccgac tgctacctcg atattcaggc ggggtctggc ggtacggaag
    66001 cacaggactg ggcgagcatg cttgagcgta tgtatctgcg ctgggcagaa tcgcgtggtt
    66061 tcaaaactga aatcatcgaa gagtcggaag gtgaagtggc gggtattaaa tccgtgacga
    66121 tcaaaatctc cggcgattac gcttacggct ggctgcgtac agaaactggc gttcaccgcc
    66181 tggtgcgtaa aagcccgttt gactccggcg gtcgtcgcca cacatcgttc agctctgcgt
    66241 ttgtttaccc ggaagttgat gatgatattg atatcgaaat caacccggcg gatctgcgca
    66301 ttgacgttta tcgcgcgtcc ggcgcgggcg gtcagcacgt taaccgtacc gaatctgcgg
    66361 tgcgtattac ccacatccca accgggatcg tgacccaatg ccagaacgac cgttcccagc
    66421 acaagaacaa agaccaggcc atgaagcaga tgaaagcgaa gctttatgaa ctggagatgc
    66481 agaagaaaaa tgccgagaaa caggcgatgg aagataacaa atccgacatc ggctggggca
    66541 gccagattcg ttcttatgtc cttgatgact cccgcattaa agatctgcgt accggggtag
    66601 aaacccgcaa cacgcaggcc gtgctggacg gcagcctgga tcaatttatc gaagcaagtt
    66661 tgaaagcagg gttatgagga accaacatgt ctgaacaaca cgcacagggc gctgacgcgg
    66721 tagtcgatct taacaatgaa ctgaaaacgc gtcgtgagaa gctggcgaac ctgcgcgagc
    66781 aggggattgc cttcccgaac gatttccgtc gcgatcatac ctctgaccaa ttgcatgcag
    66841 aattcgacgg taaagagaac gaagaactgg aagcgctgaa catcgaagtc gccgttgctg
    66901 gccgcatgat gacccgtcgt attatgggta aagcgtcttt cgttaccctg caggatgtgg
    66961 ggggccgcat tcagctgtac gttgcccgtg acgatctgcc ggaaggcgtt tacaacgagc
    67021 agttcaaaaa atgggacctc ggcgacatcc tcggcgcgaa aggtaaactg ttcaaaacca
    67081 aaaccggcga actgtctatc cactgtaccg agctgcgtct gctgaccaaa gcactgcgtc
    67141 cgctgccgga taaattccac ggcttgcagg atcaggaagc gcgctatcgt cagcgttatt
    67201 tggatctcat ctctaacgat gaatcccgta acacctttaa agtacgctcg cagatcctct
    67261 ctggtattcg ccagttcatg gtgaaccgcg gctttatgga agttgaaacg ccgatgatgc
    67321 aggtgatccc tggcggtgcc gctgcgcgtc cgtttatcac ccaccataac gcgctggatc
    67381 tcgacatgta cctgcgtatc gcgccggaac tgtacctcaa gcgtctggtg gtcggtggct
    67441 tcgagcgtgt attcgaaatc aaccgtaact tccgtaacga aggtatctcc gtacgtcata
    67501 acccagagtt caccatgatg gaactctaca tggcttacgc agattacaaa gatctgatcg
    67561 agctgaccga atcgctgttc cgtactctgg cacaggatat tctcggtaag acggaagtga
    67621 cctacggcga cgtgacgctg gacttcggta aaccgttcga aaaactgacc atgcgtgaag
    67681 cgatcaagaa atatcgcccg gaaaccgaca tggcggatct ggataacttc gactctgcga
    67741 aagcgattgc tgaatctatc ggtatccacg ttgagaagag ctggggtctg ggccgtatcg
    67801 ttaccgagat cttcgaagaa gtggcagaag cacatctgat tcagccgacc ttcattactg
    67861 aatatccggc agaagtttct ccgctggcgc gtcgtaacga cattaacccg gaaatcacag
    67921 accgctttga gttcttcatc ggtggtcgtg aaatcggtaa cggctttagc gagctgaatg
    67981 acgcggaaga tcaggcgcaa cgcttcctgg atcaggttgc cgcgaaagac gcaggtgacg
    68041 acgaagcgat gttctacgat gaagattacg ttaccgcact ggaacatggc ttaccgccga
    68101 cagcaggtct ggggattggc atcgaccgta tggtaatgct gttcaccaac agccatacca
    68161 tccgcgacgt tattctgttc ccggcgatgc gtccggtgaa ataagcatta cgttatgctc
    68221 acaaccccgg caaatgtcgg ggttttttat ttaagctggg taaatgcaga taatcgtatt
    68281 ctggcttcgc gatttgtcgc ctgcatcacc atccacggac tgaacgccca cggcgtggca
    68341 tcaataccgt gtaatacatc agctaaatca caccattgat aatccatcac ttcatcatcg
    68401 ttgatctgta acgcactggt ggtgcgtgcg gcaaataccg gacacacttc attttccaca
    68461 atgccactcg gatcggtggc gcggtagcga aagtcaggat agatagattc aggaggcgta
    68521 atttctacgc caagctcata acggcaacgg cggatcaccg cgtcttcgca gctttctccc
    68581 agttgtgggt gcccacaaac cgagttagtc cacacgccag gccacgcttt tttgctcagt
    68641 gcgcggcggg taactaataa ttgccccttg gcattaaaca gccaactgga gaacgcgaga
    68701 tgtaagaggg tgtctgccgt gtgtgcggca tacttttcca gcgtacccgt gggaactccc
    68761 tgtgcattca ataaaatgac gtgttccgtt tgcataattt ctcacatgta attctgatca
    68821 ctcatcatta tagataattg aagagtgaaa tgattaacca agtgagcgtt tatcgacaac
    68881 cgcccgtttt gagcggatgc cgacaggtaa aaaccattta aaaaagtagc atcatatatt
    68941 tgaagcgact ttcaatggcc attgcaacac agtcagaaat gacaggaata gagagagtaa
    69001 aaagcaacaa cagactgaat atagcgcgca ttaaaaaatt catattattc tccatgctca
    69061 tttttcttca gcttgcgcat cagaagccac atacgtactg tcctgacttt taaatcttta
    69121 tcagagacgg attccttcac caggtcagca tgatgctgat agccattaaa gaagacgttc
    69181 agcacaacgg cgctaagggt tgcgagcata atgccgctat gtagcagcgg ttgcagtacg
    69241 gccggtaact tagaaaagaa atcgtgagag agcgtcggag tcatgacaac gccgagactg
    69301 atcgccacaa tatagaggtt gtaacggttg gtggtgtagt tacagcgcga cagaattcga
    69361 atccctgtcg ccagtaccat gccgaacatc actagaccag cgccgcccag cacaaattgc
    69421 ggaatggagg ctaccagcac cgccattttt ggcaccatgc caaacaggat taatataatt
    69481 cccgaagaaa tacataccca gcggctatga acgcgcgtca cgctaaccag gccaacgttt
    69541 tgagaaaatg acgtgtgggg gaagctgtta aacgtgccgc ctatcattgt ccctacgcca
    69601 tcgacacgca gcccgcgaat aatatcgtgc gaagagagtt tacgaccgac tatttcaccc
    69661 agtgccagga acatccccat cgactcgata aacacgatga ttaacacggc agtcatggtc
    69721 agaatggaaa cgggatcgaa aatcggcata ccaaacgaca tcggcgtaac aatcgcaaac
    69781 catgaagcat catgtagccc ggataaattg acttcattca tcatccacga aagtaaaaag
    69841 ccaaatacaa tccccagtaa tactgcgacg ttggacataa atcctttcgc atagcgagta
    69901 atgagcaaga taaaaattaa gacggcaaag gagataccta aataaacggg attaccatat
    69961 tgcggattcc ctttacctcc ggcggcccag tcaataccca cctgaatgat gctaagcccg
    70021 atagaagtaa taaccacacc ggtaaccagt ggcgggaata aaggcatcaa gcgaccgata
    70081 agtggcgcta ataatgtggt gataaaaccc gcggcgatag tggcaccaaa tatccccagc
    70141 aggccgatat ccgggttcat cccaatggct atcattggtg ttacagcagc aaaggtcacc
    70201 gacataatca ccggcaggcg gatccccata aagcggccga taccgataca ttgcaataat
    70261 gtgacgatcc cgcagcaaaa gagatccgag ctaatgagca tcgcaatagc ttcttttgag
    70321 aggcccagtc ggtcaccaat cataagagga acagcgactg cacctgcgta catgacaagg
    70381 acgtgttgca gaccgagtat gatcagcttt cctggtgata atatgcggtc aacctcatca
    70441 gttgggcggt cttgcccaga agatgaggga agttgggaat ctatggcgct catcagtgtt
    70501 tctcctttcg cccgccagag taaaaagcaa ccgtttacgt taatgaaact gtctaaacac
    70561 atgctcgtgg agtgccagtt tttgcgagga aggggagtta gagacaggaa ttgcggaggc
    70621 aatcacataa tcgtggttaa accacgaaaa tgagaagaga acctgacaat gaaattatct
    70681 gtaattttct catattgata tttcattaat cattctggca tgtcgcactc tcgcatttaa
    70741 tcgtttttat ctggatagcg ctcttttggt cggcaatgtt gaaataatga aagcggcata
    70801 gattgagggg caataatttg aactcagtcg caaaattata attaaaacgg tcatgctacc
    70861 taatatatgc ccgtttattg catcgcgaaa tttgagaaga cataactcat taaatgaaag
    70921 ggatgcaaat gaataagttt atcgctgctg aagctgcgga atgtata
//
LOCUS       DAZKHC020000011        70902 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZKHC020000011 DAZKHC020000000
VERSION     DAZKHC020000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 70902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.011"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(214..1689)
                     /gene="ampG"
                     /locus_tag="ACHOSM_RS04490"
                     /old_locus_tag="pgaptmp_000898"
     CDS             complement(214..1689)
                     /gene="ampG"
                     /locus_tag="ACHOSM_RS04490"
                     /old_locus_tag="pgaptmp_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HGU4557474.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLTTQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1733..2311)
                     /gene="yajG"
                     /locus_tag="ACHOSM_RS04495"
                     /old_locus_tag="pgaptmp_000899"
     CDS             complement(1733..2311)
                     /gene="yajG"
                     /locus_tag="ACHOSM_RS04495"
                     /old_locus_tag="pgaptmp_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4557475.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2616..2933
                     /gene="bolA"
                     /locus_tag="ACHOSM_RS04500"
                     /old_locus_tag="pgaptmp_000900"
     CDS             2616..2933
                     /gene="bolA"
                     /locus_tag="ACHOSM_RS04500"
                     /old_locus_tag="pgaptmp_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HGU4557476.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3277..4575
                     /gene="tig"
                     /locus_tag="ACHOSM_RS04505"
                     /old_locus_tag="pgaptmp_000901"
     CDS             3277..4575
                     /gene="tig"
                     /locus_tag="ACHOSM_RS04505"
                     /old_locus_tag="pgaptmp_000901"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGU4557477.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4821..5444
                     /gene="clpP"
                     /locus_tag="ACHOSM_RS04510"
                     /old_locus_tag="pgaptmp_000902"
     CDS             4821..5444
                     /gene="clpP"
                     /locus_tag="ACHOSM_RS04510"
                     /old_locus_tag="pgaptmp_000902"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HGU4557478.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5570..6844
                     /gene="clpX"
                     /locus_tag="ACHOSM_RS04515"
                     /old_locus_tag="pgaptmp_000903"
     CDS             5570..6844
                     /gene="clpX"
                     /locus_tag="ACHOSM_RS04515"
                     /old_locus_tag="pgaptmp_000903"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HGU4557479.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DNSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            7032..9386
                     /gene="lon"
                     /locus_tag="ACHOSM_RS04520"
                     /old_locus_tag="pgaptmp_000904"
     CDS             7032..9386
                     /gene="lon"
                     /locus_tag="ACHOSM_RS04520"
                     /old_locus_tag="pgaptmp_000904"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HGU4557480.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9577..9867
                     /gene="hupB"
                     /locus_tag="ACHOSM_RS04525"
                     /old_locus_tag="pgaptmp_000905"
     CDS             9577..9867
                     /gene="hupB"
                     /locus_tag="ACHOSM_RS04525"
                     /old_locus_tag="pgaptmp_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HGU4557481.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            10059..11930
                     /gene="ppiD"
                     /locus_tag="ACHOSM_RS04530"
                     /old_locus_tag="pgaptmp_000906"
     CDS             10059..11930
                     /gene="ppiD"
                     /locus_tag="ACHOSM_RS04530"
                     /old_locus_tag="pgaptmp_000906"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU4557482.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12081..12452
                     /gene="ybaV"
                     /locus_tag="ACHOSM_RS04535"
                     /old_locus_tag="pgaptmp_000907"
     CDS             12081..12452
                     /gene="ybaV"
                     /locus_tag="ACHOSM_RS04535"
                     /old_locus_tag="pgaptmp_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGU4557483.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12546..12944
                     /gene="fadM"
                     /locus_tag="ACHOSM_RS04540"
                     /old_locus_tag="pgaptmp_000908"
     CDS             12546..12944
                     /gene="fadM"
                     /locus_tag="ACHOSM_RS04540"
                     /old_locus_tag="pgaptmp_000908"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HGU4557484.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12996..13691)
                     /gene="queC"
                     /locus_tag="ACHOSM_RS04545"
                     /old_locus_tag="pgaptmp_000909"
     CDS             complement(12996..13691)
                     /gene="queC"
                     /locus_tag="ACHOSM_RS04545"
                     /old_locus_tag="pgaptmp_000909"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HGU4557485.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(13756..15456)
                     /gene="ybaE"
                     /locus_tag="ACHOSM_RS04550"
                     /old_locus_tag="pgaptmp_000910"
     CDS             complement(13756..15456)
                     /gene="ybaE"
                     /locus_tag="ACHOSM_RS04550"
                     /old_locus_tag="pgaptmp_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HGU4557486.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLHFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15556..16374
                     /gene="cof"
                     /locus_tag="ACHOSM_RS04555"
                     /old_locus_tag="pgaptmp_000911"
     CDS             15556..16374
                     /gene="cof"
                     /locus_tag="ACHOSM_RS04555"
                     /old_locus_tag="pgaptmp_000911"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HGU4557487.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16527..16985
                     /gene="decR"
                     /locus_tag="ACHOSM_RS04560"
                     /old_locus_tag="pgaptmp_000912"
     CDS             16527..16985
                     /gene="decR"
                     /locus_tag="ACHOSM_RS04560"
                     /old_locus_tag="pgaptmp_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HGU4557488.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            17015..18787
                     /gene="mdlA"
                     /locus_tag="ACHOSM_RS04565"
                     /old_locus_tag="pgaptmp_000913"
     CDS             17015..18787
                     /gene="mdlA"
                     /locus_tag="ACHOSM_RS04565"
                     /old_locus_tag="pgaptmp_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU4557489.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QPPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            18780..20561
                     /gene="mdlB"
                     /locus_tag="ACHOSM_RS04570"
                     /old_locus_tag="pgaptmp_000914"
     CDS             18780..20561
                     /gene="mdlB"
                     /locus_tag="ACHOSM_RS04570"
                     /old_locus_tag="pgaptmp_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU4557490.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20742..21080
                     /gene="glnK"
                     /locus_tag="ACHOSM_RS04575"
                     /old_locus_tag="pgaptmp_000915"
     CDS             20742..21080
                     /gene="glnK"
                     /locus_tag="ACHOSM_RS04575"
                     /old_locus_tag="pgaptmp_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HGU4557491.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21110..22396
                     /gene="amtB"
                     /locus_tag="ACHOSM_RS04580"
                     /old_locus_tag="pgaptmp_000916"
     CDS             21110..22396
                     /gene="amtB"
                     /locus_tag="ACHOSM_RS04580"
                     /old_locus_tag="pgaptmp_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HGU4557492.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNVNW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22445..23305)
                     /gene="tesB"
                     /locus_tag="ACHOSM_RS04585"
                     /old_locus_tag="pgaptmp_000917"
     CDS             complement(22445..23305)
                     /gene="tesB"
                     /locus_tag="ACHOSM_RS04585"
                     /old_locus_tag="pgaptmp_000917"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HGU4557493.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23523..24095
                     /gene="ybaY"
                     /locus_tag="ACHOSM_RS04590"
                     /old_locus_tag="pgaptmp_000918"
     CDS             23523..24095
                     /gene="ybaY"
                     /locus_tag="ACHOSM_RS04590"
                     /old_locus_tag="pgaptmp_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HGU4557494.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24126..24437)
                     /locus_tag="ACHOSM_RS04595"
                     /old_locus_tag="pgaptmp_000919"
     CDS             complement(24126..24437)
                     /locus_tag="ACHOSM_RS04595"
                     /old_locus_tag="pgaptmp_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HGU4557495.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24600..24696
                     /gene="ffs"
                     /locus_tag="ACHOSM_RS04600"
                     /old_locus_tag="pgaptmp_000920"
     ncRNA           24600..24696
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHOSM_RS04600"
                     /old_locus_tag="pgaptmp_000920"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24816..25169
                     /gene="ybaA"
                     /locus_tag="ACHOSM_RS04605"
                     /old_locus_tag="pgaptmp_000921"
     CDS             24816..25169
                     /gene="ybaA"
                     /locus_tag="ACHOSM_RS04605"
                     /old_locus_tag="pgaptmp_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HGU4557496.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25211..26761)
                     /gene="pdeB"
                     /locus_tag="ACHOSM_RS04610"
                     /old_locus_tag="pgaptmp_000922"
     CDS             complement(25211..26761)
                     /gene="pdeB"
                     /locus_tag="ACHOSM_RS04610"
                     /old_locus_tag="pgaptmp_000922"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HGU4557497.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARRPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSAFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26925..27395)
                     /gene="ylaC"
                     /locus_tag="ACHOSM_RS04615"
                     /old_locus_tag="pgaptmp_000923"
     CDS             complement(26925..27395)
                     /gene="ylaC"
                     /locus_tag="ACHOSM_RS04615"
                     /old_locus_tag="pgaptmp_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HGU4557498.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27511..28062)
                     /gene="maa"
                     /locus_tag="ACHOSM_RS04620"
                     /old_locus_tag="pgaptmp_000924"
     CDS             complement(27511..28062)
                     /gene="maa"
                     /locus_tag="ACHOSM_RS04620"
                     /old_locus_tag="pgaptmp_000924"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HGU4557499.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(28234..28452)
                     /gene="hha"
                     /locus_tag="ACHOSM_RS04625"
                     /old_locus_tag="pgaptmp_000925"
     CDS             complement(28234..28452)
                     /gene="hha"
                     /locus_tag="ACHOSM_RS04625"
                     /old_locus_tag="pgaptmp_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HGU4557500.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28478..28852)
                     /gene="tomB"
                     /locus_tag="ACHOSM_RS04630"
                     /old_locus_tag="pgaptmp_000926"
     CDS             complement(28478..28852)
                     /gene="tomB"
                     /locus_tag="ACHOSM_RS04630"
                     /old_locus_tag="pgaptmp_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HGU4557501.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(29398..32547)
                     /gene="acrB"
                     /locus_tag="ACHOSM_RS04635"
                     /old_locus_tag="pgaptmp_000927"
     CDS             complement(29398..32547)
                     /gene="acrB"
                     /locus_tag="ACHOSM_RS04635"
                     /old_locus_tag="pgaptmp_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HGU4557502.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGEHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(32570..33763)
                     /gene="acrA"
                     /locus_tag="ACHOSM_RS04640"
                     /old_locus_tag="pgaptmp_000928"
     CDS             complement(32570..33763)
                     /gene="acrA"
                     /locus_tag="ACHOSM_RS04640"
                     /old_locus_tag="pgaptmp_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HGU4557503.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTNVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            33905..34552
                     /gene="acrR"
                     /locus_tag="ACHOSM_RS04645"
                     /old_locus_tag="pgaptmp_000929"
     CDS             33905..34552
                     /gene="acrR"
                     /locus_tag="ACHOSM_RS04645"
                     /old_locus_tag="pgaptmp_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HGU4557504.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            34680..38042
                     /gene="mscK"
                     /locus_tag="ACHOSM_RS04650"
                     /old_locus_tag="pgaptmp_000930"
     CDS             34680..38042
                     /gene="mscK"
                     /locus_tag="ACHOSM_RS04650"
                     /old_locus_tag="pgaptmp_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HGU4557505.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVVQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            38103..38201
                     /locus_tag="ACHOSM_RS04655"
                     /old_locus_tag="pgaptmp_000931"
                     /pseudo
     CDS             38103..38201
                     /locus_tag="ACHOSM_RS04655"
                     /old_locus_tag="pgaptmp_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(38254..38415)
                     /gene="rsmS"
                     /locus_tag="ACHOSM_RS04660"
                     /old_locus_tag="pgaptmp_000932"
     CDS             complement(38254..38415)
                     /gene="rsmS"
                     /locus_tag="ACHOSM_RS04660"
                     /old_locus_tag="pgaptmp_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HGU4557506.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(38429..38956)
                     /gene="priC"
                     /locus_tag="ACHOSM_RS04665"
                     /old_locus_tag="pgaptmp_000933"
     CDS             complement(38429..38956)
                     /gene="priC"
                     /locus_tag="ACHOSM_RS04665"
                     /old_locus_tag="pgaptmp_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HGU4557507.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            39026..39403
                     /gene="ybaN"
                     /locus_tag="ACHOSM_RS04670"
                     /old_locus_tag="pgaptmp_000934"
     CDS             39026..39403
                     /gene="ybaN"
                     /locus_tag="ACHOSM_RS04670"
                     /old_locus_tag="pgaptmp_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HGU4557508.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            39556..40107
                     /gene="apt"
                     /locus_tag="ACHOSM_RS04675"
                     /old_locus_tag="pgaptmp_000935"
     CDS             39556..40107
                     /gene="apt"
                     /locus_tag="ACHOSM_RS04675"
                     /old_locus_tag="pgaptmp_000935"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGU4557509.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            40236..42167
                     /gene="dnaX"
                     /locus_tag="ACHOSM_RS04680"
                     /old_locus_tag="pgaptmp_000936"
     CDS             40236..42167
                     /gene="dnaX"
                     /locus_tag="ACHOSM_RS04680"
                     /old_locus_tag="pgaptmp_000936"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGU4557510.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    41500..41564
                     /gene="dnaX"
                     /locus_tag="ACHOSM_RS04680"
                     /old_locus_tag="pgaptmp_000936"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            42220..42549
                     /gene="ybaB"
                     /locus_tag="ACHOSM_RS04685"
                     /old_locus_tag="pgaptmp_000937"
     CDS             42220..42549
                     /gene="ybaB"
                     /locus_tag="ACHOSM_RS04685"
                     /old_locus_tag="pgaptmp_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGU4557511.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            42549..43154
                     /gene="recR"
                     /locus_tag="ACHOSM_RS04690"
                     /old_locus_tag="pgaptmp_000938"
     CDS             42549..43154
                     /gene="recR"
                     /locus_tag="ACHOSM_RS04690"
                     /old_locus_tag="pgaptmp_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGU4557512.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            43264..45138
                     /gene="htpG"
                     /locus_tag="ACHOSM_RS04695"
                     /old_locus_tag="pgaptmp_000939"
     CDS             43264..45138
                     /gene="htpG"
                     /locus_tag="ACHOSM_RS04695"
                     /old_locus_tag="pgaptmp_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HGU4557513.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            45319..45963
                     /gene="adk"
                     /locus_tag="ACHOSM_RS04700"
                     /old_locus_tag="pgaptmp_000940"
     CDS             45319..45963
                     /gene="adk"
                     /locus_tag="ACHOSM_RS04700"
                     /old_locus_tag="pgaptmp_000940"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGU4557514.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            46095..47057
                     /gene="hemH"
                     /locus_tag="ACHOSM_RS04705"
                     /old_locus_tag="pgaptmp_000941"
     CDS             46095..47057
                     /gene="hemH"
                     /locus_tag="ACHOSM_RS04705"
                     /old_locus_tag="pgaptmp_000941"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGU4557515.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(47054..48013)
                     /gene="aes"
                     /locus_tag="ACHOSM_RS04710"
                     /old_locus_tag="pgaptmp_000942"
     CDS             complement(47054..48013)
                     /gene="aes"
                     /locus_tag="ACHOSM_RS04710"
                     /old_locus_tag="pgaptmp_000942"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HGU4557516.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            48165..49469
                     /gene="gsk"
                     /locus_tag="ACHOSM_RS04715"
                     /old_locus_tag="pgaptmp_000943"
     CDS             48165..49469
                     /gene="gsk"
                     /locus_tag="ACHOSM_RS04715"
                     /old_locus_tag="pgaptmp_000943"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HGU4557517.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(49602..51278)
                     /gene="ybaL"
                     /locus_tag="ACHOSM_RS04720"
                     /old_locus_tag="pgaptmp_000944"
     CDS             complement(49602..51278)
                     /gene="ybaL"
                     /locus_tag="ACHOSM_RS04720"
                     /old_locus_tag="pgaptmp_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HGU4557518.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(51516..52736)
                     /gene="fsr"
                     /locus_tag="ACHOSM_RS04725"
                     /old_locus_tag="pgaptmp_000945"
     CDS             complement(51516..52736)
                     /gene="fsr"
                     /locus_tag="ACHOSM_RS04725"
                     /old_locus_tag="pgaptmp_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HGU4557519.1"
                     /translation="MAMSEQPQPVAGAAASTTKALTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            52954..54606
                     /gene="ushA"
                     /locus_tag="ACHOSM_RS04730"
                     /old_locus_tag="pgaptmp_000946"
     CDS             52954..54606
                     /gene="ushA"
                     /locus_tag="ACHOSM_RS04730"
                     /old_locus_tag="pgaptmp_000946"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HGU4557520.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQRKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(54643..55122)
                     /gene="ybaK"
                     /locus_tag="ACHOSM_RS04735"
                     /old_locus_tag="pgaptmp_000947"
     CDS             complement(54643..55122)
                     /gene="ybaK"
                     /locus_tag="ACHOSM_RS04735"
                     /old_locus_tag="pgaptmp_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HGU4557521.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(55326..56120)
                     /gene="ybaP"
                     /locus_tag="ACHOSM_RS04740"
                     /old_locus_tag="pgaptmp_000948"
     CDS             complement(55326..56120)
                     /gene="ybaP"
                     /locus_tag="ACHOSM_RS04740"
                     /old_locus_tag="pgaptmp_000948"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HGU4557522.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            56258..56599
                     /gene="ybaQ"
                     /locus_tag="ACHOSM_RS04745"
                     /old_locus_tag="pgaptmp_000949"
     CDS             56258..56599
                     /gene="ybaQ"
                     /locus_tag="ACHOSM_RS04745"
                     /old_locus_tag="pgaptmp_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU4557523.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(56814..59318)
                     /gene="copA"
                     /locus_tag="ACHOSM_RS04750"
                     /old_locus_tag="pgaptmp_000950"
     CDS             complement(56814..59318)
                     /gene="copA"
                     /locus_tag="ACHOSM_RS04750"
                     /old_locus_tag="pgaptmp_000950"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HGU4557524.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            59580..60512
                     /gene="glsA"
                     /locus_tag="ACHOSM_RS04755"
                     /old_locus_tag="pgaptmp_000951"
     CDS             59580..60512
                     /gene="glsA"
                     /locus_tag="ACHOSM_RS04755"
                     /old_locus_tag="pgaptmp_000951"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HGU4557525.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            60515..61807
                     /gene="ybaT"
                     /locus_tag="ACHOSM_RS04760"
                     /old_locus_tag="pgaptmp_000952"
     CDS             60515..61807
                     /gene="ybaT"
                     /locus_tag="ACHOSM_RS04760"
                     /old_locus_tag="pgaptmp_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU4557526.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            61838..61935
                     /locus_tag="ACHOSM_RS04765"
                     /old_locus_tag="pgaptmp_000953"
                     /pseudo
     CDS             61838..61935
                     /locus_tag="ACHOSM_RS04765"
                     /old_locus_tag="pgaptmp_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            61932..62339
                     /gene="cueR"
                     /locus_tag="ACHOSM_RS04770"
                     /old_locus_tag="pgaptmp_000954"
     CDS             61932..62339
                     /gene="cueR"
                     /locus_tag="ACHOSM_RS04770"
                     /old_locus_tag="pgaptmp_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HGU4557527.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(62340..62798)
                     /gene="ybbJ"
                     /locus_tag="ACHOSM_RS04775"
                     /old_locus_tag="pgaptmp_000955"
     CDS             complement(62340..62798)
                     /gene="ybbJ"
                     /locus_tag="ACHOSM_RS04775"
                     /old_locus_tag="pgaptmp_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HGU4557528.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(62795..63712)
                     /gene="qmcA"
                     /locus_tag="ACHOSM_RS04780"
                     /old_locus_tag="pgaptmp_000956"
     CDS             complement(62795..63712)
                     /gene="qmcA"
                     /locus_tag="ACHOSM_RS04780"
                     /old_locus_tag="pgaptmp_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGU4557529.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            63858..64535
                     /gene="fetA"
                     /locus_tag="ACHOSM_RS04785"
                     /old_locus_tag="pgaptmp_000957"
     CDS             63858..64535
                     /gene="fetA"
                     /locus_tag="ACHOSM_RS04785"
                     /old_locus_tag="pgaptmp_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HGU4557530.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            64522..65301
                     /gene="fetB"
                     /locus_tag="ACHOSM_RS04790"
                     /old_locus_tag="pgaptmp_000958"
     CDS             64522..65301
                     /gene="fetB"
                     /locus_tag="ACHOSM_RS04790"
                     /old_locus_tag="pgaptmp_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HGU4557531.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(65364..66218)
                     /gene="cnoX"
                     /locus_tag="ACHOSM_RS04795"
                     /old_locus_tag="pgaptmp_000959"
     CDS             complement(65364..66218)
                     /gene="cnoX"
                     /locus_tag="ACHOSM_RS04795"
                     /old_locus_tag="pgaptmp_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HGU4557532.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(66279..67088)
                     /gene="ybbO"
                     /locus_tag="ACHOSM_RS04800"
                     /old_locus_tag="pgaptmp_000960"
     CDS             complement(66279..67088)
                     /gene="ybbO"
                     /locus_tag="ACHOSM_RS04800"
                     /old_locus_tag="pgaptmp_000960"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU4557533.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(67078..67701)
                     /gene="tesA"
                     /locus_tag="ACHOSM_RS04805"
                     /old_locus_tag="pgaptmp_000961"
     CDS             complement(67078..67701)
                     /gene="tesA"
                     /locus_tag="ACHOSM_RS04805"
                     /old_locus_tag="pgaptmp_000961"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HGU4557534.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            67672..68358
                     /gene="ybbA"
                     /locus_tag="ACHOSM_RS04810"
                     /old_locus_tag="pgaptmp_000962"
     CDS             67672..68358
                     /gene="ybbA"
                     /locus_tag="ACHOSM_RS04810"
                     /old_locus_tag="pgaptmp_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HGU4557535.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            68355..70769
                     /gene="ybbP"
                     /locus_tag="ACHOSM_RS04815"
                     /old_locus_tag="pgaptmp_000963"
     CDS             68355..70769
                     /gene="ybbP"
                     /locus_tag="ACHOSM_RS04815"
                     /old_locus_tag="pgaptmp_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HGU4557536.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT        17301 a        17965 c        18963 g        16673 t
ORIGIN      
        1 taacaaataa ttatcattga agagacaact gggtgatgag aaaataaaga ctcattagct
       61 gaatcgtgta agaaaaatag aatttataaa tggcgtcaat aatttccaaa cgctaagccg
      121 cacaaaagaa caaatattaa taagcgatgc tgatgtttat ttagccgtaa taattacgga
      181 gagtgattac tacagctaaa taatatttac agattacgtc agatgcgttt ttcgtagcgc
      241 cagataatcc agcaaaccac caagcacgac gccagaaagg gcgactaaaa ccccgacttc
      301 cagcagagca ggcaggaaag agaagtgcgt caaatccagc gcgtccatcg tcaacagcag
      361 taaccacacg gccaacaggc tgacgcccgc cgccagtgtc cacatggcaa aggcataacc
      421 tgccggatat tcggtacggg agataaagtt gtcatttact cgtgtatatt caagcgtctg
      481 gcggcaaacc agcagcaaaa taagccccgg tacggcagcg gcgacggaga atagatagaa
      541 tgtcgaccag ccgtgtgctt caacaaacca acccgccacg gggccgacat aaactcgtcc
      601 tacagcggaa agcgctgaga gcagggcaaa ttgagttgcg gaaaatgact tattacacag
      661 cgtcattaac agcgcgacaa aggctgatgt gcccatcccg ccacagaggt tttcgaaaaa
      721 gacggctgcg cccatgctgt agagatgctt atcagtaatc gacagcagcc agtaaccagc
      781 gttagacgca ccttgtaaaa tgccgaaaat cagcagcgcc cggaacagcg acaggcgctg
      841 catcaaaatc ccaccgtaca atgcgcccac aatggtcgcc agtaagccaa gcgttttgtt
      901 aaccacgcct acttcacccg catcaaaccc gacgccgcga atcaaaaacg tggttgtcag
      961 gctcatggcg aatgcgtcgc ccagcttata cagcacgata agaagcaaaa taagccaggc
     1021 attattgcga ccaaagaaat ctcgcagagg tgcaacaacc gcttgttcca gcgtttttgg
     1081 cacaggaatg gtgtcggtcg gttctggtgc aagcaacgtc gcgataatac aagggatcaa
     1141 cagcgccgcc atcaaccagt acatgccctg ccagcccagc catttatctg ccagccacag
     1201 ggctaagccg ccggaaacca gcatccctaa acggtaaccc agcacgctga ttgccgcgcc
     1261 cgcaccacgt tcttctgccg ggagcacatc ggttttccac gcatcgaaga caatatcctg
     1321 agaggccgag caaaaagcga tcaccaccgc cagcgccgcc atccagcgga gttgggtgcc
     1381 tggttcgaga aaacccatcg ccgcaatggc gactaataac agaatttgcg tggtgagcag
     1441 ccaaccgcgc cgccgcccaa aaaatggtgg cgtgtagcgg tccatcagcg gtgaccagag
     1501 gaatttaaaa acgtaagcct ggcctaccag agagaagaaa ccaatggttt tgagatcgat
     1561 attctcgacc gtcatccagg cctgtaaggt gccggatgtc agggcgagcg gtagcccgga
     1621 agcaaaaccc aggatcagca atatggctga acgcggctgt tgaaaaatac gtaaatattg
     1681 actggacatg ggcgtctaca ggcccggctt gcaccgggcc agagggcaga aattaacgcg
     1741 cgttctgctt gatgaattcg tggatgctgg tgtcctgaga catatcagcg atggtatcgg
     1801 tcagcacact gttaaccgca tcggcgatat ttttgttgga ggcttggaac gcaccttcaa
     1861 cgttgtagct ggcacgatag tttttggtca ttttgttgcc attctgcgcg gtagcgatga
     1921 tggcgatatc cgctttggta gcgatgttat agcgcacgtt gccctgggac acgtcagcat
     1981 acagttggct aacgatgatt tgcagattaa ccgggccatt cggaccaacc atgtaaccac
     2041 gcgcggtcat ctgtttttcc agcacttctt gcagcaggaa acgcagatcg cgggaggcgg
     2101 tcagggtaac gatttgatta tcgcgggtga cttttgccag cgcctgatcg gtacgctgat
     2161 cggcaccatt aatgcttacg gtgacgccca tcaggcttgg atcctgctgt ggcagtgtaa
     2221 tcgtcgggga aacttcaata gttgttggcg gttttgcgca tcctgcaagc ataaacagag
     2281 caactaacgg gaagaggatt tttttgaaca tgttcgggct ctcagagact cttaagcgtg
     2341 tttggtaaaa attcccgcca tcataacatt gccaacggcg aggggaagtg ggtaaggcat
     2401 gtaaattcat catgttgacg aaataatcac ccctggtaaa agaaacgctg atgcgaggcc
     2461 tgtgtttcaa tctttaaatc agtaaacttc atacgcttga cggaaaaacc aggacgaaac
     2521 ctaaatattt gttgttaagc tgcaatggaa acggtaaaag cggctagtat ttaaagggat
     2581 ggatgacatc tcagcgttgt cggaggagat atttcatgat gatacgtgag cggatagaag
     2641 aaaaattaag ggcggcgttc caacccgtat tcctcgaagt agtggatgaa agctatcgtc
     2701 acaatgtccc agccggctct gaaagccatt ttaaagttgt gctggtcagc gatcgtttta
     2761 cgggtgaacg ttttctgaat cgtcatcgaa tgatttacag tactttagcg gaggaactct
     2821 ctactaccgt tcatgcgctg gctctgcata cttacactat taaggagtgg gaagggttgc
     2881 aggacaccgt ctttgcctct cctccctgtc gtggagcagg aagcatcgcg taaaaacgca
     2941 tttgcaactg tcggcgcttt tccagtatgt tgctaaagat tttatgaaaa acggcctgcg
     3001 ggccgttttg ttttgtctgg attttgcgct ttttgcccag cattcagacg aaaattgccc
     3061 gggaattgtg aaaaaatacg cgacagcgcg caataaccgt tctcgactca taaaagtgat
     3121 gccgctataa tgccgcgtcc tatttgaatg ctttcgggat gattctggta acagggaatg
     3181 tgattgatta taagaacatc ccggttccgc gaagccaaca acctgtgctt gcggggtaag
     3241 agttgaccga gcactgtgat tttttgaggt aacaagatgc aagtttcagt tgaaaccact
     3301 caaggccttg gccgccgtgt aacgattact atcgctgctg acagcatcga gaccgctgtt
     3361 aaaagcgagc tggtcaacgt tgcgaaaaaa gtacgtattg acggcttccg caaaggcaaa
     3421 gtgccaatga atatcgttgc tcagcgttat ggcgcgtctg tacgccagga cgttctgggt
     3481 gacctgatga gccgtaactt cattgacgcc atcattaaag aaaaaatcaa tccggctggc
     3541 gcaccgactt atgttccggg cgaatacaag ctgggtgaag acttcactta ctctgtagag
     3601 tttgaagttt atccggaagt tgaactgcag ggtctggaag cgatcgaagt tgaaaaaccg
     3661 atcgttgaag tgaccgacgc tgacgttgac ggcatgctgg atactctgcg taaacagcag
     3721 gcgacctgga aagaaaaaga cggcgctgtt gaagcagaag accgcgtaac catcgacttc
     3781 accggttctg tagacggcga agagttcgaa ggcggtaaag cgtctgattt cgtactggcg
     3841 atgggccagg gtcgtatgat cccgggcttt gaagacggta tcaaaggcca caaagctggc
     3901 gaagagttca ccatcgacgt gacctttccg gaagaatacc acgcagaaaa cctgaaaggt
     3961 aaagcagcga aattcgctat caacctgaag aaagttgaag agcgtgaact gccggaactg
     4021 actgcagaat tcatcaaacg tttcggcgtt gaagatggtt ccgtagaagg tctgcgcgct
     4081 gaagtgcgta aaaacatgga gcgcgagctg aagagcgcca tccgtaaccg cgttaagtct
     4141 caggcgatcg aaggtctggt aaaagctaac gacatcgacg taccggctgc gctgatcgac
     4201 agcgaaatcg acgttctgcg tcgccaggct gcacagcgtt tcggtggcaa cgaaaaacaa
     4261 gctctggaac tgccgcgcga actgttcgaa gaacaggcta aacgccgcgt agttgttggc
     4321 ctgctgctgg gcgaagttat ccgcaccaac gagctgaaag ctgacgaaga gcgcgtgaaa
     4381 ggcctgatcg aagagatggc ttctgcgtac gaagatccga aagaagttat cgagttctac
     4441 agcaaaaaca aagagctgat ggacaacatg cgcaatgttg ctctggaaga acaggctgtt
     4501 gaagctgtac tggcgaaagc gaaagtgact gaaaaagaaa ccactttcaa cgagctgatg
     4561 aaccagcagg cgtaatttac gcagcataac gcgctaaatt cgctcaaagg cccgtcaccg
     4621 ccaggtggtg ggcttttttt tgtcatgaat tttgcatgga accgtgcgaa aagcctcttt
     4681 cggtgttagc gtaacaacaa aagattgtta tgcttgaaat atggtgatgc cgtacccata
     4741 acacagggac tagctgataa tccgtccata aggttacaat cggtacagca ggttttttca
     4801 attttatcca ggagacggaa atgtcataca gcggcgaacg agataacttt gcaccccata
     4861 tggcgctggt gccgatggtc attgaacaga cctcacgcgg tgagcgctct tttgatatct
     4921 attctcgtct acttaaggaa cgcgtcattt ttctgactgg ccaggttgaa gaccacatgg
     4981 ctaacctgat tgtggcgcag atgctgttcc tggaagcgga aaacccagaa aaagatatct
     5041 atctgtacat taactcccca ggcggggtga tcactgccgg gatgtctatc tatgacacca
     5101 tgcagtttat caagcctgat gtcagcacca tctgtatggg ccaggcggcc tcgatgggcg
     5161 ctttcttgct gaccgcaggg gcaaaaggta aacgtttttg cctgccgaat tcgcgcgtga
     5221 tgattcacca accgttgggc ggctaccagg gccaggcgac cgatatcgaa attcatgccc
     5281 gtgaaattct gaaagttaaa gggcgcatga atgaacttat ggcgcttcat acgggtcaat
     5341 cattagaaca gattgaacgt gataccgagc gcgatcgctt cctttccgcc cctgaagcgg
     5401 tggaatacgg tctggtcgat tcgattctga cccatcgtaa ttgatgccag aggcgcaact
     5461 gtgccgctat acttatccag ggcggcacaa cgctgtaagc ggcttgcgcc tgagaatggc
     5521 atttgcgtcg tcgtgtgcgg cacaaagaac aaagaagagg ttttgaccca tgacagataa
     5581 acgcaaagat ggctcaggca aattgctgta ttgctctttt tgcggcaaaa gccagcatga
     5641 agtgcgcaag ctgattgccg gtccatccgt gtatatctgc gacgaatgtg ttgatttatg
     5701 taacgacatc attcgcgaag agattaaaga agttgcaccg catcgtgaac gcagtgcgct
     5761 accgacgccg catgaaattc gcaaccacct ggacgattac gttatcggcc aggaacaggc
     5821 gaaaaaagtg ctggcggtcg cggtatacaa ccattacaaa cgtctgcgca acggcgataa
     5881 cagcaatggt gtcgagttgg gcaaaagtaa cattctgctg atcggtccga ccggttccgg
     5941 taaaacgctg ctggctgaaa cgctggcgcg cctgctggac gtcccgttca ccatggccga
     6001 cgcaaccacg ctgaccgaag ccggttatgt gggcgaagac gttgaaaaca tcattcagaa
     6061 gctgttgcaa aagtgcgatt acgacgtaca gaaagcgcag cgcgggattg tctacatcga
     6121 tgaaatcgac aagatttctc gtaagtcaga caacccgtcc attacccgag acgtttccgg
     6181 tgaaggcgta cagcaggcac tgttgaaact gatcgaaggt acggtagctg ctgttccacc
     6241 gcaaggtggg cgtaaacatc cgcagcagga attcttgcag gttgatacct ctaagatcct
     6301 gtttatttgt ggcggtgcgt ttgccggtct ggataaagtg atttcccacc gtgtagaaac
     6361 cggctccggc attggttttg gcgcgacggt aaaagcgaag tccgacaaag caagcgaagg
     6421 cgagctgctg gcgcaggttg aaccggaaga tctgatcaag tttggtctta tccctgagtt
     6481 cattggtcgt ctgccggttg tagcaacgtt gaatgaactg agcgaagaag ctctgattca
     6541 gatcctcaaa gagccgaaaa acgccctgac caagcagtat caggcgctgt ttaatctgga
     6601 aggcgtggat ctggaattcc gtgacgaggc gctggatgct atcgctaaga aagcgatggc
     6661 gcgtaaaacc ggtgcccgtg gcctgcgttc catcgtagaa gccgcactgc tcgataccat
     6721 gtacgatctg ccgtccatgg aagacgtcga aaaagtggtt atcgacgagt cggtaattga
     6781 tggtcaaagc aaaccgttgc tgatttatgg taagccggaa gcgcaacagg catctggtga
     6841 ataattaacc attcccatac aattagttaa ccaaaaaggg gggattttat ctccccttta
     6901 atttttcctc tattctcggc gttgaatgtg ggggaaacat ccccatatac tgacgtacat
     6961 gttaatagat ggcgtgaagc acagtcgtgt catctgatta cctggcggaa attaaactaa
     7021 gagagagctc tatgaatcct gagcgttctg aacgcattga aatccccgta ttgccgctgc
     7081 gcgatgtggt ggtttatccg cacatggtca tcccattatt tgtcgggcgg gaaaaatcta
     7141 tccgttgtct ggaagcggcg atggaccatg ataaaaaaat tatgctggtc gcgcagaaag
     7201 aagcttcaac ggatgagccg ggtgtaaacg atcttttcac cgtcgggacc gtggcctcta
     7261 tattgcagat gctgaaactg cctgacggca ccgtcaaagt gctggtcgag gggttacagc
     7321 gcgcgcgtat ttctgcgctc tctgacaatg gcgaacactt ttctgcgaag gcggagtatc
     7381 tggagtcgcc gaccattgat gagcgggaac aggaagtgct ggtgcgtact gcaatcagcc
     7441 agttcgaagg ctacatcaag ctgaacaaaa aaatcccacc agaagtgctg acgtcgctga
     7501 atagcatcga cgatccggcg cgtctggcgg ataccattgc tgcacatatg ccgctgaaac
     7561 tggctgacaa acagtccgtt ctggagatgt ccgacgttaa cgaacgtctg gaatatctga
     7621 tggcaatgat ggaatcggaa atcgatctgc tgcaggttga gaaacgcatt cgcaaccgcg
     7681 ttaaaaagca gatggagaaa tcccagcgtg agtactatct gaacgagcaa atgaaagcta
     7741 ttcagaaaga actcggtgaa atggacgacg cgccggacga aaacgaagcc ctgaagcgca
     7801 aaatcgacgc ggcgaagatg ccgaaagagg caaaagagaa agcggaagca gagttgcaga
     7861 agctgaaaat gatgtctccg atgtcggcag aagcgaccgt agtgcgtggt tatatcgact
     7921 ggatggtaca ggtaccgtgg aatgcgcgca gcaaggtcaa aaaagacctg cgtcaggcgc
     7981 aggaaatcct tgataccgac cattatggtc tggagcgcgt gaaagatcgc atccttgagt
     8041 accttgcggt tcaaagccgt gtcaacaaaa tcaagggacc gatcctctgc ctggtagggc
     8101 cgccgggggt aggtaaaacc tctcttggtc agtccattgc caaagccacc gggcgtaaat
     8161 atgtccgtat ggcgctgggc ggcgtgcgtg atgaagcgga aatccgtggt caccgccgta
     8221 cttacatcgg ttctatgccg ggtaaactga tccagaaaat ggcgaaagtg ggcgtgaaaa
     8281 acccgctgtt cctgctcgat gagatcgaca aaatgtcttc tgacatgcga ggcgatccgg
     8341 cctctgcact gcttgaagtg ctggatccag agcagaacgt agcgttcagc gaccactacc
     8401 tggaagtgga ttacgatctc agcgacgtga tgtttgtcgc gacgtcgaac tccatgaaca
     8461 ttccggcacc gctgctggat cgtatggaag tgattcgcct ctccggttat accgaagatg
     8521 aaaaactgaa catcgccaaa cgtcacctgc tgccgaagca gattgaacgt aatgcactga
     8581 aaaaaggtga gctgaccgtc gacgatagcg ccattatcgg cattattcgt tactacaccc
     8641 gtgaggcggg cgtgcgtggt ctggagcgtg aaatctccaa actgtgccgc aaagcggtta
     8701 agcagttact gctcgataag tcattaaaac atatcgaaat taacggcgat aacctgcatg
     8761 actacctcgg tgttcagcgt ttcgactatg gtcgcgctga taacgaaaac cgtgtcggtc
     8821 aggtaaccgg tctggcgtgg acggaagtgg gcggtgactt gctgaccatt gaaaccgcat
     8881 gtgttccggg taaaggcaaa ctgacctata ccggttcgct cggcgaagtg atgcaggagt
     8941 ctattcaggc ggcgttaacg gtggttcgtg cgcgtgcgga aaaactgggg atcaaccctg
     9001 atttttacga aaaacgtgac atccacgtcc acgtaccgga aggtgcgacg ccgaaagatg
     9061 gtccgagcgc cggtattgct atgtgcaccg cgctggtttc ttgcctgacc ggtaacccgg
     9121 ttcgtgccga tgtggcaatg accggtgaga tcactctgcg tggtcaggta ctgccgatcg
     9181 gtggtttgaa agaaaaacta ctggcagcgc atcgcggcgg gattaaaaca gtgctaattc
     9241 cgttcgaaaa taaacgcgat ctggaagaga ttcctgataa cgtaattgcc gatctggaca
     9301 ttcatcctgt gaagcgcatt gaggaagttc tgactctggc gctacaaaat gaaccgtctg
     9361 gtatgcaggt tgtgactgca aaatagtgac ctcgcgcaaa atgcactaat aaaaacaggg
     9421 ctggcaggct aattcgggct tgccagcctt tttttgtctc gctaagttag atggcggatc
     9481 gggcttgccc ttattaaggg gtgttgtaag gggatggctg gcctgatata actgctgcgc
     9541 gttcgtacct tgaaggattc aagtgcgata taaattataa agaggaagag aagagtgaat
     9601 aaatctcaat tgatcgacaa gattgctgca ggggctgata tctctaaagc tgcggctggc
     9661 cgtgcgttag atgctattat tgcttccgta actgaatctc tgaaagaagg ggatgatgta
     9721 gcactggtag gttttggtac ttttgccgtt aaagagcgtg ctgcccgtac tggccgcaac
     9781 ccgcagaccg gtaaagagat caccatcgct gctgctaaag taccgagctt ccgtgcaggt
     9841 aaagcattga aagacgcggt aaactaagcg ttgtccccgg tggggatgtg acgaagttca
     9901 agggcgcatc tactgatgtg ccttttttat ttgtattcgg tgactttctg cgtcttgtgg
     9961 gctgacaatt gcccccgttt cttgtcacaa taggcctttg cgcgcatcga tacgttgcgt
    10021 gaggtacaca gtcatctaca gcggagtgtt gttacaccat gatggacagc ttacgcacgg
    10081 ctgcaaacag tctcgtgctc aagattattt tcggtatcat tatcgtgtcg ttcatattga
    10141 ccggcgtgag tggttacctg attggcggag gcaataacta cgccgcaaaa gtgaatgacc
    10201 aggaaatcag ccgtgggcaa ttcgagaacg ctttcaacag cgagcgtaat cgcatgcagc
    10261 aacagctggg cgatcaatac tccgagctgg cagcgaacga aggctatatg aagaccctgc
    10321 gtcaacaggt gctgaatcgt ctgatcgacg aggcgctgct ggatcagtac gctcgtgaac
    10381 tgaaactggg tatcagcgat gagcaggtta aacaggcgat tttcgcgacc ccagccttcc
    10441 aggttgacgg caaatttgat aacagccgct ataacggtat cctcaaccag atggggatga
    10501 ccgccgatca gtacgcccag gcgctgcgta accagctcac tactcaacag ctgattaacg
    10561 gcgttgccgg taccgatttt atgctgaaag gtgaaaccga cgagctggcg gcactggtag
    10621 ctcagcaacg cgtggtgcgt gaagcgacta tcgatgttaa cgcgctggcg gcgaagcagc
    10681 ctgtgaccga acaggaaatt gccagctact acgaacaaaa caaaaacaat ttcatgacgc
    10741 cggaacaatt ccgcgtgagt tacatcaagc tggatgccgc aacgatgcag caaccggtta
    10801 gcgatgcgga tatccagagc tactacgatc agcatcagga tcaattcacc cagccgcagc
    10861 gtacccgcta cagcatcatc cagaccaaaa ctgaagatga agcgaaagcg gtacttgatg
    10921 agctgaataa aggcggtgat tttgctgcat tagccaaaga aaaatctgcc gatattatct
    10981 ctgctcgtaa cggcggcgat atgggttggt tagaagatgc cactatcccg gacgaactga
    11041 aaaatgctgg tctgaaagag aaaggccaac tgtctggtgt catcaaatct tcggtcggtt
    11101 tcctgattgt acgtctggac gacattcagc cagcgaaagt gaagtcgtta gacgaagtgc
    11161 gtgacgatgt cgcggcgaaa gtgaaacacg aaaaagccct cgatgcgtac tacgctctgc
    11221 agcagaaagt gagcgatgcg gcaagcaacg acaccgagtc tctggccggt gcagagcaag
    11281 ctgccggcgt taaagccact cagacgggtt ggttcagcaa agataacctg ccggaagagt
    11341 tgaacttcaa gccggttgcc gacgctatct ttaacggcgg tctggtaggt gaaaacggcg
    11401 cgccgggcat caactctgac atcatcaccg tagatggcga ccgcgcattc gtgctgcgca
    11461 tcagcgagca caaaccggaa gcggtgaaac cgttggcaga tgttcaggaa caagttaagg
    11521 cactggttca gcacaacaaa gctgaacaac aggcgaaagt agatgctgag aaactgctgg
    11581 ttgatttgaa agccggcaaa ggtgcggaag ctatgcaggc tgccggtctg aaatttggcg
    11641 agccgaaaac cttaagccgt tccggtcgtg acccgattag ccaggcggcg tttgcactgc
    11701 cactgccagc gaaagacaaa ccgagctacg gtatggcgac cgatatgcaa ggtaatgtgg
    11761 ttctgctggc gctggatgaa gtgaaacaag gttcaatgcc ggaagatcag aaaaaagcga
    11821 tggtgcaggg tatcacccag aacaacgcac aaatcgtctt tgaagctctg atgagtaacc
    11881 tgcgtaaaga ggcgaaaatc aaaattggcg atgcgctgga acagcaataa tcctgaagcc
    11941 gcctcgcaaa aaaatgcgtt gcagctgtaa caagaaaagg tcgctttcgc ggccttttcc
    12001 atttctgaac attgccattt gtttactgtt ttcactcccg ttaaggtgat tccactgtta
    12061 acaaataagg agcaaacagt atgaaacacg gaattaaagc actgctcatt accctgtccc
    12121 tggcctgtgc cggaatgtct catagcgcgc tggcggcagc ttctgtggcg aaaccgacgg
    12181 cggtagaaac caaagcggaa gctcctgcag cacaaagtaa agcagcagta ccggcgaaag
    12241 ccagtgacga agaaggcacc cgggtcagca ttaataatgc cagcgcggaa gagctagccc
    12301 gcgcgatgaa tggcgttggc ctgaagaaag cgcaggcgat tgtcagttat cgcgaagagt
    12361 acggtccgtt taaaacggtg gaggatctaa agcaggtgcc ggggatgggc aattcgctgg
    12421 tggaacgtaa tctggcggta ttaaccctgt aattaatttg catagtggca attttttgcc
    12481 agactgaaga ggtcatacca gttatgacct ctgtacttat aacaacaacg taaggttatt
    12541 gcgctatgca aacacaaatc aaagttcgtg gatatcatct cgacgtttac cagcacgtca
    12601 acaacgcccg ctaccttgaa tttctcgaag aagcccgctg ggatgggttg gaaaatagcg
    12661 acagttttca gtggatgacg gcccataaca tcgccttcgt cgtggtcaat atcaatatta
    12721 actatcgtcg cccagcggta ttaagcgacc tgttaactat taccagtcag ttgcagcaat
    12781 taaacggtaa aagcggcatc ttaagccagg tcattacact ggagccggaa gggcaggtgg
    12841 tagcggatgc gcttattacg tttgtttgta ttgatcttaa aacgcagaaa gcattagctc
    12901 tggaagggga attgcgcgaa aagctggagc agatggttaa gtaaacgttt tgtggtgccg
    12961 gatgctcaag ccgcatccgg cgacacccgg aataattacc tcaacccggt tttctgcttc
    13021 atcgctgcca tcaccgtcgg tttatcggcc agataatgat tcaaaccgtt ggcgcgtaaa
    13081 ttacatgccg cacaatgacc gcaaccgtcg cctttaatgc cgttatagca ggtcaacgtt
    13141 tcgttacgga ctaaatccag tttgccgtaa taatctgcca gcgcccaggt ttccgcttta
    13201 tcaatccaca tcagcggcgt ttcaaaacga atatctttcg ccatgcccaa actgacggca
    13261 tggtttagtg ctttcacaaa ctcatcgcgg caatccgggt agccggagaa atccgtttca
    13321 cagacgccgg taattacggc ttctgctttt acctgatacg catatattgc cgccagcgtc
    13381 aggaacaaaa tattacgccc agggacaaac gtattcggga tgccatcggc ttcaggttca
    13441 taatcaggca ccggaatgct gtcacgtgtc aggctgctga ccgccagctc gttgagcagc
    13501 gttacatcaa gaaccttatg cgcgcgtgct cccagtttca gcgccagttc gcgtgccacg
    13561 tcgatttctg cgcgatgtcg ctgaccgtaa tcgaacgtca cgcaatggac ttcatcatat
    13621 tgttgtaatg cctgcaccag acaggtggtg gaatcctggc ctccactgaa cacaacgaca
    13681 gcacgtttca tagataatcc tgcttgaaca ataaaagcgt tatggtaacg cctgcgatta
    13741 acccggacca gctattcatt gcgacggcgc gggaagccag gcttcggtaa attcaaacca
    13801 gccgcgcggt gtcagtcgca caccgttcac gccgggaggg gcactaatgc gatagtgata
    13861 gttgaacagc ggcgtcagcg tcgcgtctgc cattaactgg ctaaaaaccg ctttcagggc
    13921 attaaatcgg ttttcctcat caggcattac ttgcaccgcg tccagcgtcg attgcagatg
    13981 tgcatatgct ggagcgtcga aaacatgtgg ccatagtgga tcgcaacgta gccattgctc
    14041 cagagtatat tccggtgctt cgccaattaa tctgtcgccc atcatgaggt ctgcgtgtgc
    14101 cagtagggtc gtgtcgtccc agttttttgc gttatgaaaa ataattgtga gttcacagcc
    14161 ttccgctgcc agtgtcgcct gtaggcgttc tgccatggta tgaagttcta tcggtaggtg
    14221 ataaaccagc gtcaaggttt tcggtagttt gacttcatcc ggtacttgcc agtgcggaat
    14281 agtccagcct ggcagtaatg catgactggc ggtgatcagg ttttctccga cttctaacgt
    14341 ttgtaataaa ccggattgat gaataatgga gatcactttt cgcgcctgcc agagggagag
    14401 tcggggactt ttgcgcaacg ttaaatagca aaaacctaaa ctgatgccac tactgacctg
    14461 gctgacccgt tgcagctctt ccggtttgcc gatggtgatt tgcacgggat gccgacaact
    14521 ggttcccaaa tctttttcga aaagcggcgg agttatccag tactcaaccg ctttaagcag
    14581 cggatgacgc aaatggtaat aatcatggct ctccaggcgc accagctccg ctgtgaattg
    14641 tgttaagcga aaaggaccag tgccgatcag tgggaattgc ggatgcgcca gatggctgca
    14701 atagctcgcc agccggtgcg caagccagta atcggggcga tgtaaaaaga aggtcagaca
    14761 ctgcggatgg gtgacttcaa tacgcttcac gctaataaat aattgatcca gtgctggcag
    14821 ttgtaacagc atcaataatc gctggtgtaa gtgtgaggct tttactgcat cgccgttatg
    14881 ccagtgtagg gttgaacgaa gataaaagtc ccagcgtaac ccgtcagtag aggtttccca
    14941 gtgatgcgct aaatcgccaa tcgggcgctg agtattatta tcgaagcggg tcaggccgga
    15001 aaatatctgc ccggcgagat gctgctcggc acggccgggc aaaaagcctg gttgtagcgg
    15061 ttcgagcggg cgatagtagg gaatacgcaa cgtgggtgta tcgttttgcc attgtccgcc
    15121 cataaacggc tgtaacagag tgcgcagctc acctggggcc agttgcgcca gctccagcac
    15181 atcttgctgc tttccggttt ccagtgcctg ttccatcatc gcattgcgta gcgattccgg
    15241 cgtgaccaga aagtgtaatt gtccgcgctt tccgcgtcct gactgcgcct gccactccag
    15301 ccatcccgcc tcctgtgcct gacgcaacag cgtacgaaca tggcgttcgc tgcaaaaaca
    15361 gcgttcggct agttcgctga cggtgacggt ttgcggcttt ccggctgaag gttgccacag
    15421 acgttgatac tggttaagac ggttgagcaa tcgcatataa acccggaaca atattattta
    15481 actattcact attacttccg tatatatcag gtgatactca atcaccatta accgtgtcac
    15541 agagtggaga aagaaatggc tcgtctggca gcatttgata tggatggcac tttattgatg
    15601 cctgaccatc atttaggtga gaaaaccctc tctactttgg cgcgactgcg tgaacgcgac
    15661 attaccctca cttttgccac ggggcgtcat gcgctggaga tgcagcatat tctcggggcg
    15721 ctatcgctgg atgcatattt gattaccggc aacggaacgc gcgtgcattc tctggaaggt
    15781 gaacttttac atcgtgatga tttacctgcg gatgtcgcgg agctggtgct gtatcagcaa
    15841 tgggataccc gagccagtat gcatatcttc aatgacgacg gttggtttac cgggaaagag
    15901 atccctgcgt tgttgcaggc atttgtctat agcggttttc gttatcagat aatcgatgtc
    15961 aaaaaaatgc cactcggcag cgtcaccaag atctgcttct gtggcgatca cgacgatctt
    16021 acacgcttgc agatccagct atacgaagca ttaggcgagc gtgcacattt gtgtttttcc
    16081 gccacggatt gcctcgaagt gctgccggtg ggctgcaata aaggcgctgc attgacggtg
    16141 ctgacccaac atttaggttt atcgttgcgc gattgcatgg cctttggtga tgcgatgaac
    16201 gatcgcgaaa tgttaggcag cgtcggtagc gggtttatta tgggcaatgc gatgccgcaa
    16261 ctgcgcgcgg agctcccgca tttaccggtg attggacatt gccgaaatca ggctgtctct
    16321 cactatttga cgcactggct ggactatcca catctacctt attcccccga ataacgagat
    16381 cccttccagc accgggcaat tgcccggttt tttttgcgtt gaatttgtca ttttgtgccg
    16441 tggtgtttaa accgcacaga ataaattgtc gtgatttcac ctttaaaata gaattaaaag
    16501 agaaaaaaat tctctgtgga agggctatgt tagataaaat tgaccgtaag ctgctggcct
    16561 tactgcagca ggattgcacc ctctctttgc aggcactggc tgaagccgtt aatctgacaa
    16621 ccaccccttg ctggaagcgc ctgaaacggc tggaggacga cggtatcctt atcggcaaag
    16681 tcgccctgct ggatccggaa aaaataggcc tcggcctgac cgcttttgtg ctgataaaaa
    16741 cgcaacatca cagcagcgaa tggtattgcc gctttgtcac ggtggttacc gaaatgccag
    16801 aagtgctggg gttctggcgc atggctggtg aatacgatta tctgatgcgc gtccaggttg
    16861 ccgacatgaa acgctacgac gagttttata agcgtctggt aaacagcgtg ccggggctgt
    16921 cggacgtcac ttccagcttc gcgatggaac agattaaata caccacttct ttacccatcg
    16981 aataaatatc cagaatcagg tcaggacaca acgcgtgcga ttatttgctc aattaagctg
    17041 gtatttccgt cgggaatggc gtcgctatct cggggctgtc gccttgcttg tcattatcgc
    17101 gatgctgcaa ctggttccgc caaaagtggt tggtattgtt gtcgatggcg tgacagaaca
    17161 acactttact accgggcaga tcctgatgtg gatcgccacc atggtgctga ttgccgttgt
    17221 ggtttatctc ctgcgttacg tctggcgggt attgctgttt ggcgcgtctt accaactggc
    17281 tgttgaactg cgtgaagatt attaccgtca gctaagccgg cagcccccgg agttttacct
    17341 gcgtcatcgc acgggtgatc tcatggctcg tgcgaccaat gacgtcgatc gcgtcgtgtt
    17401 tgccgccgga gaaggagtgc tgacgctggt ggattcactg gtaatgggct gcgctgtgtt
    17461 gattatgatg tctacgcaaa ttagctggca gttgacctta tttgccctgt tgccgatgcc
    17521 agtgatggcg atcatgatta agcgcaacgg cgatgctttg catgaacgct ttaagctggc
    17581 acaggcggcg ttttccagtc ttaatgaccg cacccaggaa agcctcacca gtatccgcat
    17641 gatcaaagcc tttggtctgg aagatcgcca gtcggcgtta tttgccgcgg atgccgaaga
    17701 taccggcaaa aaaaacatgc gggtggcgcg tattgatgct cgtttcgacc cgaccatcta
    17761 tatcgcgatt ggtatggcga acttgctggc gattggcggc ggtagttgga tggtggtgca
    17821 gggcagttta acgctgggcc agctcaccag ttttatgatg tatttaggtc tgatgatttg
    17881 gccaatgttg gcgctggcat ggatgtttaa cattgtggaa cgtggtagtg ctgcgtacag
    17941 ccgtattcgc gcgatgctgg cggaagcgcc ggtggtgaac gatggtagcg aacccgtgcc
    18001 ggaagggcgt ggcgaactgg atgtaaatat tcaccagttc acgtatccgc agactgacca
    18061 tcctgcgctg gaaaacgtca atttcgccct gaaacccggt cagatgctgg gtatctgcgg
    18121 gccgactggt tccggcaaaa gtaccctgtt gtcgctcatt cagcgtcatt tcgacgtcag
    18181 cgagggggat attcgctttc atgatattcc tctgacgaag ttacaactcg atagctggcg
    18241 tagccgcctg gcggtggtca gccagacgcc attccttttt tctgacaccg tggcaaataa
    18301 catcgcgctg ggttgcccga atgccaccca gcaagagatt gagcatgtcg cgcggttagc
    18361 cagcgtacat gacgatattt tgcgtttacc gcaaggttac gatacagagg tgggcgagcg
    18421 cggcgtgatg ctttccggcg ggcaaaaaca gcgtatctct attgctcgtg cgttattagt
    18481 caacgcggaa atcctcatcc ttgatgatgc gctttcggcg gtggacggac gcacagagca
    18541 ccagatcctg cataacctgc gtcagtgggg acagggaaga acggtaatca tcagtgccca
    18601 tcgcctttct gcactgacgg aagccagtga aattattgtg atgcagcacg gacatatcgc
    18661 ccagcgtggc aatcatgatg tgctggcgca acaaagcggc tggtatcgcg atatgtatcg
    18721 ctatcaacaa ctggaggcgg cgctcgacga cgctccggaa attcgcgagg aggccgtcga
    18781 tgcgtagttt tagccaactg tggccgactc tcaagcgcct gttagcgtat ggttcgccgt
    18841 ggcgtaaacc gctggggatt gcggtcctga tgatgtgggt tgcggcggcg gcagaagtca
    18901 gtgggccgct gcttatcagc tattttatcg acaatatggt agcgaaaaat aacctgccgt
    18961 tgaaagtggt tgcagggctg gctgcggcgt atgttgggct acaactgttt gccgccgggc
    19021 tacattacgc gcagtcgctg ctgtttaatc gggcggcagt aggcgtagtg caacagttgc
    19081 gtaccgacgt gatggatgct gcgttacgcc agccattaag cgagtttgat acccaacccg
    19141 tcgggcaggt gatttcccgc gtcactaatg acactgaagt gatccgcgat ctctacgtta
    19201 ccgtagtggc aactgtcctg cgtagcgccg cactggtggg cgcgatgctg gtggcgatgt
    19261 tcagcctcga ctggcgaatg gcgctggtgg cgataatgat tttcccggtg gtgctggtgg
    19321 taatggtgat ataccagcgt tacagcacgc cgattgtccg tcgtgtgcgc gcctatctgg
    19381 cggatatcaa cgacggcttt aacgaaatca tcaatggcat gagcgttatc cagcagttcc
    19441 gtcagcaggc gcgatttggc gaacgtatgg gggaggccag tcgttcacac tatatggcca
    19501 ggatgcaaac cctgcgcctc gacggttttc tgctgcgtcc gctgctgagt ctgttttcat
    19561 cgctcattct ttgtggcttg ttgatgctgt ttggcttctc cgctagcggc accattgaag
    19621 tgggcgtgct gtatgcgttt atcagctatc ttgggcgact taacgaacca ttaatcgaac
    19681 tgaccacgca acaggcgatg ctgcaacagg ctgttgttgc tggtgagcgc gtgtttgaac
    19741 tgatggacgg accgcgccag caatatggca atgatgatcg cccgttacag agtggcacca
    19801 tcgaagtcga taacgtgtca tttgcttatc gcgatgacaa tctggtgcta aagaacatta
    19861 atctctctgt gccttcgcgt aactttgtgg cactggtcgg gcataccggc agtggcaaaa
    19921 gcaccctcgc cagtttattg atgggctatt acccgctaac ggaaggtgag attcgccttg
    19981 atggtcgtcc attaagttcg ctaagtcaca gcgcgctgcg ccagggcgtg gcaatggtgc
    20041 agcaagatcc ggtggtgctg gcggatacct tcctcgccaa cgtgacgctg gggcgtgata
    20101 tctccgaaga acgcgtctgg caggcgctgg aaaccgtgca actggcggag ctggcgcgca
    20161 gcatgagtga tggtatttat acgccgctgg gcgagcaggg gaataatctc tccgtcgggc
    20221 aaaagcaact gctggcactg gcacgcgtgt tggtggagac gccgcaaatc ctgatccttg
    20281 atgaggcaac cgccagcatt gactccggta ctgaacaggc gattcaacat gctctggcgg
    20341 ctgtgcgtga acataccacg ctggtggtga ttgctcaccg cttatcgacc attgttgatg
    20401 ccgacaccat tctggtgctt catcgtgggc aagccgtgga gcagggcact caccagcaac
    20461 tgctggcggc ccagggacgc tactggcaga tgtatcaact gcaacttgcg ggcgaagagc
    20521 tggcagccag cgtgcgtgaa gaagaatcat tgagcgcctg aatagcgcaa tatttcatcg
    20581 ttggtgcaaa aatgtaacgc actgtgcact gtcatagtgc gttttcattt tcaatcttct
    20641 taacttcctg ctctctttct cgtttttcat ttctggcaca ccgcttgcaa taccttcttc
    20701 gtgtagcaga accattaccg aattctgacc ggaggggatc tatgaagctg gtgaccgtga
    20761 taatcaaacc attcaagctg gaagacgttc gtgaagcgtt atcttccatt ggtattcagg
    20821 gcctgaccgt caccgaagtg aaaggtttcg ggcgtcagaa agggcatgcc gagctgtacc
    20881 ggggggcgga atacagcgtc aacttcctgc caaaagtaaa aattgatgtg gcaattgctg
    20941 atgaccaact cgatgaagtg atcgatatcg tcagtaaggc ggcttacacc ggaaaaattg
    21001 gcgacggcaa aatcttcgtc gctgaattgc aacgcgtcat tcgtattcgt accggcgaag
    21061 ccgacgaagc ggcgctgtaa tctctggcac acagcaacag gaacgaaaaa tgaagatagc
    21121 gacgataaaa actgggcttg cttcactggc gatgctgccg ggactggtaa tggctgcacc
    21181 tgcggtggcc gataaagccg acaatgcgtt tatgatgatt tgtactgcgc tggtgctgtt
    21241 tatgactatt ccggggattg ccctgtttta cggtgggttg attcgcggca aaaacgtgct
    21301 gtcgatgctg acgcaggtga cggtgacgtt tgcactggtc tgtattctct gggtggttta
    21361 cggttactcg ctggcgtttg gtgagggtaa caacttcttc ggcaacgtta actggttgat
    21421 gctgaaaaac atcgaactga cggcggtgat gggcagcatt tatcagtata tccacgtagc
    21481 gtttcaggga tcgtttgcct gcattaccgt aggcttgata gttggggcgc tggcggaacg
    21541 aatccgcttc tcagctgtgt tgattttcgt ggtggtatgg ctgacgctct cttacattcc
    21601 gattgcgcat atggtgtggg gcggtggttt gctggcttct cacggtgcgc tggatttcgc
    21661 gggtggcacg gtggtgcaca ttaacgcagc aatcgccggt ctggtgggcg cgtatctgat
    21721 aggcaaacgc gtgggcttcg gtaaagaggc gtttaaaccg cacaacctgc cgatggtctt
    21781 caccgggact gccattctct atatcggttg gtttggcttt aacgccgggt cagcgggcac
    21841 ggcgaatgaa atcgcggcac tggcatttgt gaatactgtg gtcgcaacgg cggcggcaat
    21901 tcttggctgg atcttcggtg aatgggcgct gcgtggtaag ccttctctgc tgggagcgtg
    21961 ttctggcgcg attgccggtc tggtcggcgt gacgccagcg tgcggctaca ttggggttgg
    22021 cggcgcgttg attatcggcg tggtagctgg tctggcgggt ttgtggggcg ttaccatgct
    22081 caaacgcttg ctgcgggtgg atgatccctg cgatgtcttc ggtgtgcacg gcgtttgtgg
    22141 cattgtcggc tgtatcatga ccgggatttt tgccgccagt tctctgggcg gcgtgggctt
    22201 cgctgaaggt gtgacgatgg gccatcagtt gctggtacag ctggaaagca tcgccattac
    22261 gatcgtctgg tccggtgtgg tggcatttat tggctacaaa ttggcggatc tgacggttgg
    22321 tctgcgtgta ccggaagagc aggagcgaga agggctggat gtcaacagtc acggcgagaa
    22381 tgcctataac gcgtaacaag cactgcaaaa aacagccgga cggttttcac ctccggctat
    22441 ttttttaatt gtgattacgc atcacccctt cctgaacggt cgaggcaacc agtacgccgt
    22501 cctgggtata aaactcaccg cgcacaaagc cacgtgcgct ggacgccgag gtgctctcca
    22561 cgctatacag cagccattca ttcaaattaa acgggcgatg gaaccacatg gaatggtcaa
    22621 tggtggcaat ctgaatcccc ggttcgagaa aaccgatgcc gtgcggctgt agagctaccg
    22681 gcaggaagtt aagatcagaa gcgtaaccga gcagatactg atgaacgcgc aggtcatccg
    22741 gcacgctacc atttgcgcgg atccacacct gacgatgtgg ttctgcgacg tgacctttca
    22801 gtgggttatg aaactccacc ggacggactt ccagcggacg atcgcagatg aatttatctt
    22861 taagcactgg cggcagcagg tgcgccagcg attgggcgat ttgcgtttcc gaaggcaggc
    22921 catcaggcgc tggcgcggac ggcattgttt tttgatgttc gaaacccgct tctggtgcct
    22981 ggaaagaggc cgtcatataa aaaatcggtt tgccgttttg aatagcagca acccggcggg
    23041 cgctgaagct gttaccgtca cgcagcgttt cgacatcata aataatcggt ttcttactat
    23101 cgcctgggcg aagaaagtag ctgtgaaacg aatgtaccag ccgctcttcc gggacggtct
    23161 cttttgcagc atacaaggcc tgacccacga cctggccgcc aaacacctgg cgtaaaccta
    23221 aatcttcact ctggccgcga aagagtcctt cctcaatttt ttccagattt aacaatgtca
    23281 gtaaattttt tagcgcctga ctcatataac tctccagtaa caaagctgcc gcagcaagcc
    23341 aaagtgagtt gagtataacg caaatttgcc actggtccga tgggtgcaat aatctgaatt
    23401 acgggctaat tacaggcaga aatgcgtgat gtgtgccaca cttattgacg ttactatttt
    23461 gttaaccact cttccggcga ggaaagttag cccgctggtg cattgataat aaggagaaat
    23521 gaatgaaact cgtgcacatg gccagtggtt tagcggttgc gattgcgttg gcggcttgcg
    23581 cagataaaag cgcggatatt cagacgccag ccccggctgc aaatacgtct atttcagcaa
    23641 cacaacaacc tgctatccag caaccgaatg tctccggtac cgtctggatc cggcagaaag
    23701 tcgcactgcc gcctgatgct gtgctgaccg tgacactttc tgacgcgtcg ttagccgatg
    23761 caccgtcaaa agtgctggcg cagaaagcgg tgcgtaccga aggtaaacag tcaccattca
    23821 gctttgttct gccatttaac ccggcagatg ttcagccgaa cgcgcgtatt ctgttgagtg
    23881 cggcgattac cgtgaatgac aaactggtat ttattaccga taccgttcag ccggtgatca
    23941 acaagggcgg aactaaagcc gacctgacat tggtgccggt gcagcaaacc gccgtgccgg
    24001 ttcaggccag cggtggtgca acgactaccg taccttcgac ttcaccaact caggtgaatc
    24061 cgtcttcggc agttccagct cctacgcaat attaagcgac gttaaccctc tccgcccgga
    24121 gagggttagt agttccagcg ataacgctgc aagtcgattt gcccgcttcc cgataccatc
    24181 acaccttctg ccagtaatgc ctgtcgctga cgctgtaaat ccggtccggt tagcgaaatt
    24241 gtgccgtggc gattaaccac ccggtgccag ggtaaggtgc tgccttcagg gagacgcttt
    24301 aacacaccgc ccacctggcg cgcggcgcgg ggcgatcccg ccagtttcgc cacatcaccg
    24361 taagtggtga catagccttc gggaatagcg gcgacgattt gccagacgcg ttggggaaat
    24421 gaatcttctt tttccatctt ttcttcctga ggtaattttt cagcataatc tggaaaaacg
    24481 cccgagtgaa gtcgcattgc gcaagaaacc agcatctggc acgcgatggg ttgcaattag
    24541 ccggggcagc agtgataatg cgcctgcgcg ttggttctta acgctctcaa tgggggctct
    24601 gttggttctc ccgcaacgct actctgttta ccaggtcagg tccggaagga agcagccaag
    24661 gcagatgacg cgtgtgccgg gatgtagctg gcagggcccc cacccatttc tgcctcccac
    24721 cgtttcgtca aaaaaaccca acatggctaa actttaacca caactgacgt cgcaagaatt
    24781 gtctggctgc gcagtacgct tcggaggtat gtctgatgaa gtatgttgat ggttttgtgg
    24841 ttgccgttcc tgccgataaa aaggatgcct atcgggaaat ggccgctaag gctgcgccat
    24901 tgtttaaaga gtttggcgcg cttcgtattg tcgaatgctg ggccagcgat gtaccggatg
    24961 gcaaagtgac cgattttcgt atggcggtga aagcggaaga gaatgaagag gttgtcttta
    25021 gctggattga atacccttca aaagaggtcc gcgacgctgc taatcaaaag atgatgtcgg
    25081 acccacggat gaaagagttc ggcgagtcca tgccgtttga cggcaagcga atgatctatg
    25141 gcggattcga gtcaatcatc gacgaatagg tagcgtgacg ggctgtgcgg cgtgccgtca
    25201 gcccaaaaat tcacaaatgt tgctcggccc agcgtaagaa atcttctttc ggtaatgcct
    25261 tgctgtagag ccagccctga ccgtagtgca cgccatgctg gcgtagccac tcttcttgtt
    25321 tactggtttc gattccctcc gctaccattt tcagtttcag tgtttttgcc atttcgatga
    25381 tatgcggcgt gacattttta tattccagcg catcaacgaa agatttatcg atcttcagaa
    25441 tgtcgacatc cagatcctgt aaataactta aacttgaata tcccgtacca aaatcatcaa
    25501 gataaatttc atggcccgcc tcccggtagc gagaaattat cggggcgctg gttttcggat
    25561 cggcaaactc gcgttcagtg agttcaagcg cgatctgtct gggattaacc tgatagtgat
    25621 tgatcatttc acgcagcaat tgcgggattt tttccgaggt gagcacggtg gattcaagat
    25681 tgatcgaaat atgctgctgt ggatgctgac gcagccagtc gcccatatct tcaaaggcgc
    25741 ttcttataat cagtagcgtc aatggctcag aaaggcccgt ttgctgtgcc agcggaataa
    25801 aactatctgg tgacaaccaa ctaccgtctg tctgcggcct gcgcgccagt gcctcagcac
    25861 cgacaatttt gccattggct aaggagacaa tcggctgata gtgcacgcaa atatcacgat
    25921 tttcgatagc atcctgcagc cgatgatgcg gtgactggat acggcgcaaa atacgcagca
    25981 caaacatcgc tgccagcagg ccaatcacca acccgagcgg taaccaaata aagacttgcc
    26041 gatgccagcc tttctgcaac attttcgttg aagcccaggt gatgatcgaa atattcatct
    26101 ccgggaaggg caggatatcg taaatgattc cattattttc gatatgctca ccgggtgttt
    26161 tttgtagcct ggtaataatt ccctgagcaa tttcatcgct gctggttatg acaacgttat
    26221 gggcattgcc aataatggcg gcatcaattt gccatgagct atagggaatg acatcaataa
    26281 aggaagcggg gtcgatcatg acgacataat gtgccgttcc catggcgacc atgtaacgga
    26341 taatgcctaa atcgttatgc gatgttaacc agacacgata accatctttc gagattttac
    26401 ctggctcggg gaaggtatcg ggcggacttt catgctccag agacgaacac tggggaacgt
    26461 tgttatcgat ataaaccact tcctgaatat agcgataact gtaagatacc cgacgcattt
    26521 ccatgagatg ggcttcgcta caggcagcgc cttgccatct ttccagctct tgcagcgcat
    26581 ctttcccttg tgtcgccacc ttattggccc gaatagcgac gcgggaggaa taggtatcca
    26641 gctcttcaat aaacgatgtt tctacctgct gatgggccag ccagatgctt aagccgacag
    26701 gcagcaatac tgaaagaatc agtactcccg aaataaggcc gaccagatgt cgtgttctca
    26761 ccagcatatc cttattaaca cagtgtaggg aaagtatatc tgattgtgga aaaaggctgg
    26821 caggagataa ggaggtagag ctggtatttc atgaagtgat gggtggaaac gatcattgtc
    26881 atttgaccag gcgcagagct ttacgcctgg tatgtaaccc taacttaaga tgttgattcg
    26941 gcgcgagcga gggtaaaaat atcgtaaaaa gacagttcac ctttacggac gatcattttt
    27001 tgcagttgtt ccttatgaac atccgcgaca cgcggagagt tcaagatggc ttcaaccagc
    27061 gcggcaggta caaagcgcgt gttgtaccat tcgccgttat agcaaacgcg gaaatccagc
    27121 acgtcgatat cttcatagcg gtagcgtggg tagacatcga aaaagaagaa accattaagc
    27181 aggataaata atacaaccag tagccagaca gagcctgaca gcgtttcgga ctgcaacatc
    27241 accgccaggg tggcaaaaaa agcaacgtac ataccgataa acagccccgg atgtttacgg
    27301 ataaaactga tactaaagcg gggtttgttg tcgcgttttt cgcgggtatt cagagactca
    27361 atcgtttcgg tcagcaggcg ttgtatttcg gtcatcgttt gccttcgtgg tatctgcaac
    27421 tttccaggga acaccctatt ttaacgtggg aatagtagag gggaagtaac agatctacca
    27481 caattttgca taacagttgc gaaaaaccga ttacaatttt ttaattattc tggctggatt
    27541 accgcccacg acaacgttgt ccgggacacc ttttgtgaca actgcacctg aagctaccac
    27601 gacgttatca ccaatggtca caccagggtt aatgaccgcg cgtccgccaa tccagacgtt
    27661 attaccgatg gtgacgggtt tccccagttc agcaccgcta ttacgtgcta cagggtcgat
    27721 gggatgtgtt gccgtgtaga tatgaacgcc tggtgccaac atacagttat caccgatgcg
    27781 aatagggcag acatcaagca tcacgcaatc gaagttggcg aaaaaattat tgccgagaaa
    27841 aatgttatag ccataatcac agcgaaacgt tggctcaata taagcctctg tcacctgacc
    27901 gaataaatca gcgagaattt gctggcgtaa tgtgtgctct tccgccaggg aatgattgta
    27961 tcggtgaata agctgacgag cgcgcaggcg atcgcgagat aacgtctcat ctgccgagcg
    28021 atacaactca ccagcaatca tcttttcttt ttctgtgctc attactgacc tccggttgct
    28081 gaaaatgcgg caacacagta agacgaaaag tcagtgaaca gaacgttccc attacatctg
    28141 tgtgataaag atcacatagg gtatgtcttc atggtgaaaa agtataaaat tcttaataaa
    28201 cagccggtta tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga
    28261 agggatcttg tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga
    28321 gtaaaatacc gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc
    28381 cagcgtgtca attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg
    28441 tttttcggac atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat
    28501 tctctttcgt cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct
    28561 gaagatcctg catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt
    28621 cgtcgatctg ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga
    28681 aggtcgcaat atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt
    28741 taacccagcc atgattgctt tcttcaaggt ttgcaagaca gtcatgatac agggtttcac
    28801 agagaaactt aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc
    28861 cccttcttag tggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg
    28921 acttgccctc tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt
    28981 attacgaaaa attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac
    29041 taacccgcag caggtaaaag cagtttgctc gaactgttca taacatttca ggattatcgc
    29101 cagatgaaat tgatgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga
    29161 atgaataaag aattaccgca aataattaag aatagcctct aaatgattat ggattattaa
    29221 atctattagc gctacttaat ataaattaac taacaatcag tattttttat gaattttatc
    29281 cgtggttaat actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg
    29341 ttcgtaggtt atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca
    29401 atgatgatcg acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg
    29461 aaccaccaca aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc
    29521 gcccattaca ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac
    29581 cagcggcata acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat
    29641 ccgcactgca tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt
    29701 ggcgaattcg acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc
    29761 tacctggaag taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat
    29821 aacccccagc ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc
    29881 cgccagacac aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc
    29941 ggagaggcgt tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt
    30001 gctcgccagt tgttccatta gctccattgc ttcaccggta cttttacccg gtgccgcctg
    30061 gcctaagatt tccatggatg gcaggccatt gtaacgttcc agacgcggcg aaccgtactc
    30121 ccaacgagaa gaggagaatg ccgagaacgg caccatctga ccatcagcag cacgaacata
    30181 ccagtcgccg atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt
    30241 cttcacacga ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag
    30301 agtggtgtta atgtcattga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc
    30361 aatcttaaac tgcggggtat cttccagacc gtttggacgt acgctggtca acatatcagg
    30421 gtgcttcgct gcttctgcaa gcaactggtt acgcgcctga gtcagttttt cgtgaccaag
    30481 gccagcctgg tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc
    30541 gggcaggtta aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg
    30601 catggtaatc gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga
    30661 aacgaacgca ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa
    30721 caccgactca acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac
    30781 tttctgcgta cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc
    30841 ctggtcctca tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac
    30901 cacgatgatc agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct
    30961 gtcggtgtag tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt
    31021 tttaccttcc ccgtgctcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg
    31081 agtcaggatc aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa
    31141 ctgacgatag atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga
    31201 cagtaccatc gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc
    31261 ttcttttggc ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat
    31321 agcgtcatcc accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat
    31381 cgagaagcca aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat
    31441 ggtcggaatc aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag
    31501 gatgatcgct tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt
    31561 ggtgtcgtac gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag
    31621 ttcagcacgg attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc
    31681 cagaccggaa gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag
    31741 ctcaatcttc gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag
    31801 gattttgccg aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag
    31861 ctgttggcct ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg
    31921 cgctttgatg gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg
    31981 gttcatccag atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga
    32041 cgtacggctg atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt
    32101 catggtgcca tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc
    32161 aacgctcacc ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt
    32221 gttctgcacc tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat
    32281 ctgcacggta cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt
    32341 catattctgt tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg
    32401 gtaggaggcg gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag
    32461 tttgaggatc gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat
    32521 cgggcgatcg ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttag
    32581 actgttcagg ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg
    32641 cttttacctg gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg
    32701 ctttcagacc ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac
    32761 gggtttccac tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac
    32821 gggttacgcc ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg
    32881 cgcgcacgaa catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg
    32941 tgatagaccc agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg
    33001 ggaacttaat gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca
    33061 gcgtgccatt cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat
    33121 caacgtagat cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta
    33181 ccaatgcgcc ttccgtcacg ttcgacttac caatgcgacc gctaatcgga gaggtgacgt
    33241 tggtgtaagc cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag
    33301 cattcgcctg ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag
    33361 taccgagcag tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg
    33421 ctttcgccag atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct
    33481 gatagagaga gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa
    33541 tcccgctaac ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct
    33601 cggttgtgat ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct
    33661 ggccaccttg ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca
    33721 tcagaacgac cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt
    33781 ccgatttcaa attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc
    33841 gtcgtgctat ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga
    33901 acatatggca cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt
    33961 ggctctacgt cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa
    34021 agcagctggc gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt
    34081 cagtgagatc tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc
    34141 aaaattccct ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc
    34201 cacggtgaca gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt
    34261 tgtcggagaa atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga
    34321 ccgtatagaa caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat
    34381 gacgcgtcgg gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct
    34441 ctttgccccg caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact
    34501 ggagatgtat ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat
    34561 ctgactccag gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg
    34621 atattcttgc ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca
    34681 tgactatgtt ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt
    34741 ttgtttttgt cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc
    34801 tgccgacaaa agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt
    34861 ctgctcagga caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa
    34921 tcgatcgcat aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccggaaa
    34981 aaatgcgcca ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa
    35041 cgcgcaaaat tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gtccaggcgc
    35101 tggacgattt gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt
    35161 tacagacgca gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac
    35221 aaattcgcag tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga
    35281 aagtgttaat gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa
    35341 gcctggaagg gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg
    35401 cgaacagcgc ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc
    35461 gcctgacttt aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta
    35521 ttcaggctaa tccgttggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc
    35581 tgattaccgc gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact
    35641 ggctggagcg ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg
    35701 gcagcctgct gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg
    35761 aactggaaaa catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc
    35821 agcagcgtga tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca
    35881 ccaacgaagt caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg
    35941 aattgctgga tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc
    36001 aaatcaacca gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc
    36061 aaatcttttg ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc
    36121 aaagcctgaa agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc
    36181 ctgccgtttt tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc
    36241 actggcgtct gggctggctg aaagcgtatc aacaaaaact ggcttccgct gtgggttccc
    36301 tgcgtaacga tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc
    36361 tgccggtgtg cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca
    36421 tcagcgaact gctatggtcg ttcagcaaaa aactggcgat tttctggctg gtgtttggcc
    36481 tgtgctggaa ggtactggag aagaacggcg ttgccgtgcg tcacttcggc atgccggaac
    36541 agcagaccag ccactggcgt cggcaaattg tccgcatcag tctcgcattg ctgcctatcc
    36601 atttctggtc tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc
    36661 aagcgatgat tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg
    36721 aaagctggcg tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga
    36781 ttatcccgat tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc
    36841 tctccgggcg ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga
    36901 cggtattgcg cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc
    36961 gtcggcagaa tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca
    37021 ttgcactgga acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc
    37081 tgttcggtgt catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg
    37141 acagcatcac gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg
    37201 tcaccatggg cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc
    37261 gcaacctgcc tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg
    37321 cgtcgtatgc cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg
    37381 tgttcggatc gctgggcgtc tcttgggata aactccagtg gctggccgca gcattatccg
    37441 taggtcttgg ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc
    37501 tattcgaacg tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg
    37561 taagtaagat ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca
    37621 tcccgaacaa agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta
    37681 cgcgtctggt gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag
    37741 tgttactgaa ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct
    37801 tctttacggc gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac
    37861 tgcgtgaccg tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg
    37921 aaaacgacat caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg
    37981 atgaggtgac ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt
    38041 aataaccaaa gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc
    38101 atgccggatg cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt
    38161 gcaggagcgg tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa
    38221 tcttagggga catttagcgg ccccatttat ttctcacttt tccgcctcat catcgcgcgt
    38281 taatttcttt tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg
    38341 gatctgattt tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc
    38401 attttccagt gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga
    38461 ttttttccag cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat
    38521 gcagcgtttg ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa
    38581 ccatctcacg tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg
    38641 caattttcgg tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga
    38701 tggcttccag ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct
    38761 gctcaactgc atgacgaagc gcagccagat tatcgcccgc ctcatcgaga caagcctgca
    38821 gtgttgtcgc acgggtttga aaaaggtgtc tgtcaaaacg ggcacttagc gtggcaaatt
    38881 gcgctaccgg ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca
    38941 gcagggcggt tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc
    39001 actctgtaag ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct
    39061 ggcggtagtg ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat
    39121 cctgctggcg gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta
    39181 ccgctcatgg tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg
    39241 cgtcaaaccg cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt
    39301 ccagatgcca tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat
    39361 gtggcgaatt ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt
    39421 tgcaattatt gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca
    39481 aaggttaaac aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca
    39541 agcaggcata cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc
    39601 aaaagcattc aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg
    39661 gaagacccga aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg
    39721 ggcattacca aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct
    39781 ctgggtctgg gcgttggctt tgtgccggtc cgtaaaccgg gcaaactgcc gcgtgaaact
    39841 atcagtgaaa cttacgatct ggaatacggc accgatcagc tggagatcca cgttgatgcc
    39901 atcaaaccgg gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc
    39961 gaagcgaccg ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt
    40021 atcaacctgt tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac
    40081 agccttgtcc cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga
    40141 cagcacaaga tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt
    40201 cgtgaatcca ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa
    40261 aatggcgccc acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg
    40321 cgaacggctt gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg
    40381 tcggaaaaac ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta
    40441 ccgcgacgcc gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg
    40501 atctgattga aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg
    40561 ataacgtcca gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc
    40621 atatgctgtc gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc
    40681 acgttaagtt tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcac
    40741 gctgtctgca atttcatctc aaggcgctgg atgtcgagca aattcgccat cagcttgagc
    40801 acatcctcaa cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg
    40861 ccgctgaagg cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg
    40921 acggccaggt ttcaacccag gcagtcagtg cgatgctggg tacgcttgat gacgatcagg
    40981 cgctgtcgct ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta
    41041 atgaagccgc tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt
    41101 tgcatcgtat tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca
    41161 ttgagctgcg gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact
    41221 atcagacgct gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg
    41281 ttgagatgac gctgctgcgc gcgctggcgt tccatccacg tatgcctctg cctgagccag
    41341 aagtgccgcg ccagtccttt gcacccgtcg cgccaacggc agtaatgacg ccaacccagg
    41401 tgccgccgca accgcaatca gcgccgcagc aggcaccgac tgtaccgctc ccggaaacca
    41461 ccagccaggt gctggcggcg cgccagcagt tgcagcgcgt gcagggagca accaaagcaa
    41521 aaaagagtga accggcagcc gctacccgcg cgcggccggt gaataacgct gcgctggaaa
    41581 gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg ctggaaaaag
    41641 cgccagccaa aaaagaagcg tatcgctgga aggcgaccac tccggtgatg cagcaaaaag
    41701 aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa acgccggaac
    41761 tggcggcgaa gctagcggca gaagccattg agcgcgaccc gtgggcggca caggtgagcc
    41821 aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa gaggagagcg
    41881 acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac cgcggtgcac
    41941 agcaaaaact ggctgaagcg ttgagcatgt taaaaggttc aacggttgaa ctgactatcg
    42001 ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg atatacgaag
    42061 aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag accctgcgtc
    42121 ggtttttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg taagcacagc
    42181 ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa aggcggtctg
    42241 ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat gcaggaagag
    42301 atcgcgcagc tggaagtcac cggcgaatct ggcgcaggtc tggtaaaagt gaccatcaac
    42361 ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga cgacaaagag
    42421 atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat tgaagaaacg
    42481 cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg ctttaagatg
    42541 ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg cgctgtctgc
    42601 cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag cgcgatcgta
    42661 gcggcgggat gcgtctggcg caggcgctca cccgggcgat gtcggaaatc ggccactgcg
    42721 ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat ccgcgtcgtc
    42781 aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catctacgcc attgagcaga
    42841 cggggcagtt ttctggtcgt tattttgtgt tgatggggca tctgtcaccg ctggacggca
    42901 tcggtccgga tgatatcggg cttgatcgtc tggaacagcg tctggcagag gaaaaaatca
    42961 ctgaagtgat cctcgccacc aatcccacgg ttgaaggtga agctaccgct aactacattg
    43021 ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc gtaccggttg
    43081 gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ctcccttgcc gggcgtcata
    43141 agattcgttt ttaagcaaac gagagcagga tcacctgctc tcgcttgaaa ttattctccc
    43201 ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt gaggtagacc
    43261 tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct tctgcacctg
    43321 atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat ctctaacgcc
    43381 tccgatgcgg cggacaagct gcgtttccgt gcgctatcta acccggacct gtacgaaggt
    43441 gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgcacgct gaccatctcc
    43501 gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac tatcgctaaa
    43561 tccggtacca aatcattcct cgaatccctg ggttctgacc aggcgaaaga cagccagctg
    43621 atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa agtgaccgtg
    43681 cgtactcgcg cggcaggcga aaaaccagaa aacggcgtct tctgggaatc ggctggcgaa
    43741 ggtgaatata ccgttgccga catcaccaaa gaagatcgtg gtactgaaat caccctgcat
    43801 ctgcgtgaag gcgaagacga gttcctcgat gactggcgcg tgcgttccat catcagcaaa
    43861 tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa agacggcgaa
    43921 accgttatct cctgggagaa aatcaacaaa gcgcaggcgc tgtggactcg taacaagtcg
    43981 gaaatcaccg atgaagagta caaagagttc tacaaacaca tcgcccacga ctttaatgat
    44041 ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag cctgctgtac
    44101 atcccgtccc aggctccgtg ggatatgtgg aaccgtgatc ataaacacgg cctgaaactg
    44161 tacgtccagc gtgtgttcat catggacgac gcagaacagt tcatgccgaa ctatctgcgc
    44221 ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg tgaaatcctc
    44281 caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt gctgcaaatg
    44341 ctggaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttctggca acagtttggc
    44401 ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat cgccaaactg
    44461 ctgcgttttg cttctaccca taccgattct tctgcgcaga ccgtatctct ggaagactac
    44521 gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcaccgcaga cagctatgcg
    44581 gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga agttctgctg
    44641 ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga cggtaaaccg
    44701 ttccagtcgg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga agttgatgag
    44761 agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgtgtgaa agccctgctc
    44821 ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc agcgatcgtt
    44881 tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc ggcgggccag
    44941 aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact ggtgaaacgt
    45001 gcggcagata ctgaagatga agcgaagttc agcgagtggg tagaactgct gctggatcag
    45061 gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg tcgtatgaac
    45121 cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc atttttcttt
    45181 tcatcatctg cactttccgc aaattatctc gccattaacc gtttcagcct caggtgcctt
    45241 tcttgaggca atcgcctgtt ggtggtatcg tttatcgctt tttcaaaaaa ttcgacacat
    45301 tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc ggggaaaggg
    45361 actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac tggcgatatg
    45421 ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga cattatggat
    45481 gctggcaaac tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg cattgctcag
    45541 gaagactgcc gtaatggttt cctgttggac ggcttcccgc gtaccattcc gcaggcagac
    45601 gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt accggacgaa
    45661 ctgattgttg atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg tgtttatcac
    45721 gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga agaactgact
    45781 acccgtaaag acgatcagga agaaaccgta cgtaaacgtc tggttgaata ccatcagatg
    45841 actgcaccgc tgatcggcta ctactccaaa gaagcggaag cgggtaacac caaatacgcg
    45901 aaagttgacg gcaccaagcc ggttgctgaa gttcgcgctg atctggaaaa aatcctcggc
    45961 taatcaagcc gcagcagatc tgctttcggg caggtctgct catccacctc aggcaatacc
    46021 tctcacagca attagttctt cttcctcact tttccgctac aattatcaac aagttgaatc
    46081 gataagaggc ggtaatgcgt cagactaaaa ccggtatcct gctggcaaac ctgggtacgc
    46141 ccgatgcccc cacacctgaa gcggtaaaac gctatctgaa acaattttta agcgacagac
    46201 gcgtggttga tacctcacgg ttgttatggt ggccattgct gcgtggcgtt attttgccgc
    46261 tgcgctcgcc gcgtgtggcg aagctgtatg cctctgtctg gatggaaggt ggctcgccgc
    46321 tgatggttta cagccgccag caacagcagg cgctggcaca acgtttaccg gagacgcccg
    46381 tagcgctggg aatgagctac ggctcgccat cactggaaag cgccgtcgat gaactcctgg
    46441 cagagcatgt agatcatatt gtggtgctgc cgctttatcc gcaattctcc tgttctacgg
    46501 tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg caaacgtagc attccgggga
    46561 tatcgtttat tcgtgattac gccgataacc acgattacat taatgcactg gcgaacagcg
    46621 tacgcgcttc ttttgccaaa catggcgaac cggatctgct gctgctctct tatcatggca
    46681 ttccccagcg ttatgcagat gaaggcgatg attacccgca acgttgccgc acaacgactc
    46741 gcgaactggc ttccgcactg gggatggcac cggaaaaagt gatgatgacc tttcagtcgc
    46801 gctttggccg ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa atgctcggag
    46861 aaaaaggcgt aggtcatata caggtgatgt gcccgggctt tgctgcggat tgtctggaga
    46921 cgctggaaga gattgccgag caaaaccgtg aggtcttcct cggtgccggc gggaaaaaat
    46981 atgaatatat tccagcgctt aatgccacgc cggaacatat cgaaatgatg gctaatcttg
    47041 ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga agagcctcgt
    47101 cggcggtttt catcattcgt gaataatgca aaaaggcatg cagcgtacct gggtagagtt
    47161 tgaactcaca gggctgctga tgcgccgcta acgtctggta aagcagacgg ctgtcatcca
    47221 gcagcggatc gaactccgcc ccggcaataa aacagggcgg aacttcgcga gtgagatcat
    47281 tattaaacag acagtaatac ggtgactcgc ggtccgcgtc gttgcttaaa tacgcctctt
    47341 catacatctg caaatcctgt tgcgttaagc catcccagac accgcccaac agacgacgag
    47401 tcacggaatc ccgtaatccg taaagcccat accacagcaa aacacccgca attttaccgc
    47461 aatcgatctg tttatcacgc aaccacaacg cactggcgag cgccagcatg gcacctgcgg
    47521 aatcaccggc aaagccaatg cgggacatat tgatttgata atcctccgcc tgctggtgga
    47581 aataacaaca agcagccaca atttcctcta tcgcttgcgg aaaacgcgct tcaggtgaaa
    47641 gggggtaatc aataccaatc accgtacatt ggctgtagct tgccagcagg cgcatgatgc
    47701 gatcgtgggt atcgagattg ccgagaataa aaccgcctcc atgcaaataa aatagcgtcg
    47761 ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat tttgttggaa
    47821 ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg cgctcaagcg
    47881 tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt aaatccggct
    47941 gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca ggtagtttgt
    48001 tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac cactgccatg
    48061 aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct cccgtcagat
    48121 tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa tttcccggta
    48181 aacgtaaatc caaacattac ttccctgtaa atgcacgcga tccgctgctt cagcaattcc
    48241 agccagaaaa cgaaaccagc gccgcctggg tagtgggtat cgatcaaacg ttggtcgata
    48301 ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc gggcattcac
    48361 tggtgattga ggatgatgta gccgaagcgc tttatcagga actaaaacag aaaaacctga
    48421 ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcataactac tcggtgctcg
    48481 cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt ggcagctatg
    48541 cctatcgtta cctgtgtaac acctccagcc gtaccgatct taactatcta caaggcgtgg
    48601 atggcccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt acctttgcta
    48661 tcagccctgg ccacatgaac cagctgcggg ctgaaagtat tccggaagat gtgattgccg
    48721 gagcctcggc tctggttctc acctcttatc tggtgcgttg caagccgggt gaacccatgc
    48781 cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg gtggtgctga
    48841 cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa ttcctcaaag
    48901 accacgtctc tatccttgcg atgaacgaag atgaagccga agcgttgacc ggagaaagcg
    48961 atccgttgtt ggcatctgac aaggcgctgg actgggtaga tctggtgctg tgcaccgccg
    49021 ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt aaaacccagc
    49081 atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc cgcgccatgc
    49141 gccacaagga ttgccagaat ccgctgcgtg tatattcgca cattgcgccg tacatgggcg
    49201 ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca gcgttgctgc
    49261 atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc aaacataaat
    49321 tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac cgtgtgagct
    49381 atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag cgtgaagaca
    49441 gcctggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc agataaggcg
    49501 ttcacgccgc atctggcatt tggctctcga tgcccgatgc gacgctggcg cgtcttatca
    49561 tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt caccacttca
    49621 cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg ttcacccatc
    49681 actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata atgggcgcgg
    49741 gcgataatct caatatccgg gtttttcgcg cgggcagatg ccacaatctc gcctgcttca
    49801 taaccgttgg gaatcgtcag gatcagccat tttgcacatt ccagatgcgc cagttgcata
    49861 atttcttcgt tcgccgcatt gcccaatact gcgcggaccc cgcgctctcg cagctcatca
    49921 acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag caatttctcc
    49981 cccagcaggc tgcctacacg accgtaacca accagtagcg catggttgca aatatccact
    50041 gggatctgct tctcttcttc gattgcctct tccagcgtct gctcttccag cgtttcggtc
    50101 ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat cgacaggatc
    50161 gctcctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc cattcccagt
    50221 cccgccagaa taaaagcgaa ctcaccaatc tgcgccaggc tggcggcgat ggttaatgcc
    50281 gtgcgttggg agtgaccaaa cagacgcaca aggaacaggg ccgcgaccga tttaccaaac
    50341 agaataattg ccagcgtcgc cagtactgct agcggttgct gaatcagaat taacggatca
    50401 aacagcatcc cgacggagac aaaaaacagc accgcaaacg cgtcgcgcaa tggcagcgta
    50461 tcgtgggcgg cacggtgact cagttcagac tcgttcagta ccatcccggc aaagaacgca
    50521 ccgagtgcaa aggagacatc aaacagctct accgcaccaa aggcaatccc taacgccagc
    50581 gccagcaccg acagggtaaa cagctcgcga gaaccggttg ccgcgctgcg tgccataatc
    50641 cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat caccttgccg
    50701 atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccttg ttccatcatt
    50761 cctgccactg cgggcagcaa caccagcgtc agaaccatca ccaggtcttc cacaatcaac
    50821 caaccgatgg cgatttgccc acgctgactg tctattaatt gccgttcttc aagtgcgcgc
    50881 agtaacacca cggtactggc ggtggaaaga cataaaccga acacgatacc ggtcattaac
    50941 gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc tatctgggcg
    51001 atcgcgccgg gaatggcgat ggactttacc gccatcaaat ccttgagcga aaaatgcaaa
    51061 ccgacgccga acatcagcaa aataacgccc agttcagcca gttccggcgc aagcttggta
    51121 tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag atatcccacc
    51181 agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag cacaaggccg
    51241 ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt tcctggtggt
    51301 tattgtccat ttttggccgg gaaaaccaaa attacaggta atagtttatg acaatttcat
    51361 tgatgatgtt catgaataat tattgaattt tgcagaaaaa tggaattagc tgcaaaaaaa
    51421 gcacggatcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaac
    51481 gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg gttatcaggc
    51541 aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata gactaactcg
    51601 atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc catgccaaaa
    51661 gcaaaaccga aaaagagtcc agaaaccata ccgatacgtc ccggaagtag ctcctgagcg
    51721 tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc
    51781 cccgtccagt gcaggctggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata
    51841 gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta
    51901 cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat
    51961 ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat
    52021 ttcgagaaaa tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt
    52081 ggattgatta tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa
    52141 cggctaattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg
    52201 ttgcctttgc cataaggcgc gataatcacc gccgccagca agggtcccag ggaactgcca
    52261 aagttgccgc cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata
    52321 cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc
    52381 gccagcagaa ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta
    52441 aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc
    52501 actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata
    52561 aatgtcagag aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc
    52621 atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttagggcc
    52681 ttggtcgttg acgcagccgc gcccgccaca ggctggggtt gttcactcat tgccatagga
    52741 aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact
    52801 aacatacctg tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct
    52861 ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt
    52921 tcaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg
    52981 ttagcgctgt taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat
    53041 aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat
    53101 gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag
    53161 gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg
    53221 cccgagtctg acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat
    53281 gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag
    53341 gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag
    53401 cgcctgttta aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg
    53461 ctgacaaccg atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt
    53521 cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca
    53581 gacattatta tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac
    53641 gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc
    53701 ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac
    53761 gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat
    53821 gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg
    53881 gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa
    53941 agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat ctcgctgtta
    54001 tcaccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcga aaccaatggt
    54061 cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt
    54121 ctggcagccc aaatggatcg cactggtgcc gactttgcgg tgatgagcgg aggcggaatt
    54181 cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt gcagccattc
    54241 ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg tgattgatta cctgaccgcc
    54301 gtcgcgcaga ggaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg
    54361 gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga tccggcgaaa
    54421 acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata tccgcgcctt
    54481 gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat
    54541 atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg
    54601 cagtaatccg aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc ggcgggcgat
    54661 atcagcaaat ttggcatcaa ggatctttgc cagatcgcct gccgccagtt cgatatccag
    54721 tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat
    54781 cgttggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga
    54841 acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac
    54901 ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc
    54961 accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt
    55021 gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa
    55081 cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta
    55141 aacaaaaaaa cgaagcaagc atacgcctga tggcagtcca aaaaaaagag tcatcttgcc
    55201 taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt
    55261 gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca
    55321 tttttttagc gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc
    55381 gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc
    55441 tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta
    55501 ttttgcggag gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta
    55561 tgccagtggg tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac
    55621 atggtaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc
    55681 gcctgcaata actggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc
    55741 gtcgcctgaa gaaccatcgc gatttgccac agcggttgag tagaaaagag cgacggagaa
    55801 atgcccatct cctggctaat gtgctgcaga ttttggagtt gctcctcgtt aatgcgctct
    55861 tccagcgcct cgcaagtagg caaattagca aaaggcgtat cgctggtgga aacatccgcc
    55921 tcgacgatca gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc
    55981 atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt gccgggaagg
    56041 gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt
    56101 acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga
    56161 gggatggcgc aaatggagtt ggggacttat ttaatgtgtt tgtatgattc agtatgttct
    56221 tgcatcgcta ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc
    56281 gacgacaccc ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa
    56341 tgagttagca gagttgctgc atgttcatcg taatagcgtc agtgcactga tcaataacaa
    56401 tcgtaaactc accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga
    56461 ttttaggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac
    56521 ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg
    56581 tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt
    56641 cacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacccg tcttatcagg
    56701 cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc
    56761 atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc
    56821 ttcggtttaa accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc
    56881 attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca caaaataccg
    56941 gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg
    57001 ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc
    57061 atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg
    57121 acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga
    57181 cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca
    57241 atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg
    57301 gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca
    57361 ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc
    57421 acaggcgttg ctccttgcga tgcctgagca gtaatctccg cttcgatagc tttggtacca
    57481 acctgttgct catttaacag cgcctgattg cccagcaata acgcatgacc ttcagcttca
    57541 ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggcag ctgcatatca
    57601 cctgctttat cgaggatcgc ccgtgccagc ggatggctgg aaccttgctc cagtgccgcc
    57661 gccagacgca atgcctgcgc ttcatcaaca tcagcaaacg ttttcactgc gacaacctgc
    57721 ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg
    57781 cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg
    57841 gaaataatcg acatcggcgt cgccagcccc agcgcacacg gacaggcgat aatcagtacc
    57901 gtggtggcaa tcaccagggt ataaacaatt tgcggtgccg gaccaaagaa gtaccagatt
    57961 gccgcactga ccagcgcaat caccaccacc acaggtacaa atacggctga gattttatcc
    58021 gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatccgt
    58081 gacagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacgctacc gtcctgtacc
    58141 actgtcccgg catggacgct ttcgccttcg cctttttgct gcgggattgg ttcgcccgtc
    58201 agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga
    58261 tcgccggtcg tcaggcgcag taacatccct ggctgaactt ccgccagcgg cacgcttttt
    58321 tcaccctctg cggtgaccac tcgcgccgtc ggtggggtta aatcgagtaa tttctccagc
    58381 gccttagaag aacgctgacg tgcgcgcgct tccagcatat ggccgagatt aatcagaccg
    58441 ataatcatcg cactggcttc atagtaaaga tgccgcgctt ccatcgggaa ccactgcggc
    58501 cacaggttga cgctcatcga atagagccac gccacacccg ttcccagcgc caccagcgta
    58561 tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg
    58621 gcgaaaacca tcactgccag ggttatcagg ccgataacta accacaagct gcggttgtca
    58681 gcggtgacca tcatgttatc gccgatcatc ccccagacca ttaccgggat acccacagcc
    58741 agtgcaacaa ttgcctgcca gcggaagcgc ttcatcgtag ctacggcggt ttcttgctgg
    58801 cgctcgcggc gtttagcgtc atcttcaatc gcttccgcac cgtagcccgc tttttccacc
    58861 gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg
    58921 tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca
    58981 cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc
    59041 gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc
    59101 agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg
    59161 atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgcc
    59221 tgctcaacat ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag
    59281 gacaggccgt ccagggtcag gtcgatagtt tgtgacataa aacactcctt taagacagtt
    59341 ttgactggct gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa
    59401 ccaggcgagt aaaagtccgt aaagattaaa aaattggctc aatttgcatc aggattagac
    59461 atttatctct ttgttttcct gtagttaagt tgcgtgtgct aagttaaatc aggatgcctg
    59521 aaaatcggca ccggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga
    59581 tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac
    59641 ttaacggcgg acaaaatgcc gactacattc cctttctggc gaatgtacct ggtcaactgg
    59701 cagcagtggc tattgtgacc tgcgatggca acatctatcg cgcgggtgat agtgattacc
    59761 gctttgcgct ggaatccatc tcgaaagtct gtacgttagc ccttgcgtta gaagatgtcg
    59821 gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag
    59881 ttatcgcctt agagttgcat ggcggcaaac cactgtcgcc actggtaaat gctggcgcta
    59941 ttgccaccac cagcctgatt aacgctgaaa atattgaaca acgctggcag cgaattttac
    60001 atatccaaca gcaactggct ggtgagcagg tagcgctctc tgatgaagtc aaccagtcgg
    60061 aacaaacaac caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc
    60121 tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctcc acgctcatca
    60181 atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgctga
    60241 cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg
    60301 aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg
    60361 gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac
    60421 caccgctgga cgaagaaggc aacagtgttc gcggtcaaaa aatggtggca tcggtcgcta
    60481 agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg
    60541 taacaaacct ctcggtctat ggaacgtcgt ttccatcggc attggggcaa tggtgggggc
    60601 ggggatcttc gcgctgctgg ggcaggctgc attgctaatg gaagcctcga cctgggtcgc
    60661 ctttgctttt ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc
    60721 gagctatccc agcaatggcg gcattatcga cttctttcgt cgcggattag gcaacggcgt
    60781 cttttcgctg gcgctctcgt tactgtacct gttgacgctg gcggtgagca tcgccatggt
    60841 cgcccgtgct tttggcgctt atgccgtgca gtttttacat gaaggcagcc aggaggagca
    60901 ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag
    60961 caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt
    61021 attgctgatt attgccggtg tctggtcgct gcaaccagcg catatttccg tctctgcgcc
    61081 ccccagctcc ggtgcgttct tctcctgtat tgggataact ttccttgcct atgcgggctt
    61141 tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc
    61201 gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcac tggttttgct
    61261 tagcgatgta tcggcattag agttagaaaa atatgccgat accgccgtag cgcaggctgc
    61321 ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac
    61381 ggcttcagcc attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag
    61441 cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa
    61501 catcattgtc gtggtgttga ttatgctgat gacggcggca ctgaatttag gctcactcgc
    61561 cagcgttgcc agcgccacct ttttgatttg ctacctggcg gtgtttgtgg tggcgatccg
    61621 cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt
    61681 gttggtgatc gttggcttta tctacagtct gtggtcccag ggtagccgtg cgttgatatg
    61741 gattattggc tcactcttac tcagccttat tgtggcaatg gtcatgaagc gcaataaaac
    61801 cgtataacat ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg
    61861 cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg
    61921 ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgact agcaaagcca
    61981 ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc
    62041 gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg
    62101 gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca
    62161 gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg
    62221 agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca
    62281 gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat
    62341 taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatgc
    62401 gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata
    62461 tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc
    62521 ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat
    62581 ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc
    62641 caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac
    62701 aacaaataac catttccgcc cagcatctcg gctgccagca gcaaaccgcc gagactgagc
    62761 cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc
    62821 tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg
    62881 gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct tcggtgtatt
    62941 tctgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca
    63001 ttttggtggc gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg
    63061 ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga
    63121 ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg
    63181 ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt
    63241 caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc
    63301 gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg
    63361 tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg acttcatagg
    63421 cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt
    63481 tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct
    63541 tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg
    63601 tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg
    63661 cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa
    63721 cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg
    63781 attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc
    63841 agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat
    63901 atctggcggg tgatgcgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat
    63961 ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt
    64021 cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa
    64081 agccagaaat ctaccgccag caagtctctt actgcgccca gacaccgacg ctgtttggcg
    64141 atacggtata cgataatctg atctttccct ggcagatccg taaccagcag cctgacccgg
    64201 ccatttttct cgattttctc gaacgcttcg ctttgccgga cagcattttg acgaagaata
    64261 tcgccgagct atctggtggt gaaaaacaac gcatctcatt gattcgtaac ctgcaattta
    64321 tgccgaaggt tttattgctg gatgaaataa ccagtgcgct ggatgaaagt aataaacata
    64381 acgtcaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga
    64441 cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg
    64501 gagaaatgca ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg
    64561 gcattaatgc tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag
    64621 aaagatattc tctggagcgt cgggcgagcg ataattcagc tgattattgt cggctatgtg
    64681 ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc
    64741 tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc
    64801 tcatcgttta ttgccattac ggtcggggcg gggattaccc tggcggtgct gattctctca
    64861 gggtcgattg aatttatccc gatgcaggtg atccctatcg ccgggatgat tgccggtaac
    64921 gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag
    64981 caacagattc aggagaaact gagtcttggt gcgacgccga agcaggcttc agcgatattg
    65041 attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc
    65101 ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag
    65161 gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc
    65221 attattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg
    65281 acgcaattga agaagaaata atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg
    65341 ttcatgtgcc gatcaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc
    65401 gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc
    65461 tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct
    65521 tcattgcgcc caacctggtg cagttgcaga gccagttgcg tcgccagtgc ggcatcttct
    65581 ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcgtatcagc cgcctgcttc
    65641 agcagttcga tttgcgccac cagcccctgg tagcgggtgt cctgatcctg caacggaatg
    65701 gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt ttctgccagc
    65761 agcaggccga tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg
    65821 gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct
    65881 tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg
    65941 aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc gcgcagacca
    66001 aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat aaattgcccg
    66061 ttgtactgcg ccgcgaggct ttccaggatc ggggttaact gcaaacagtg ctggctacgt
    66121 tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg
    66181 ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat
    66241 attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc
    66301 atcacgcgcc ccggcagcag gcgcttgagc accattactg cccaggtcac cagcgttacc
    66361 ggatagcgca tcttcggctt ctcgctgata aaagcatggc gtactttgtc caccaccgct
    66421 tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc
    66481 gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact
    66541 ttaattccgc tgtggcgcag ctccatgcgc agtgcgtctg accacgcctc cagcgcatat
    66601 ttactggccg cgtaagcgcc acgacccggc gtggagatta atcccatcac cgatgatgtc
    66661 atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg
    66721 tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct gatggtggaa
    66781 agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc
    66841 agggcgatca cctcgtctgc tgcgcgatca acactttctg gtgaatccag atcgatcaac
    66901 acgccggtaa atcccatgtt gttcatacgc tcaacatcat ccggttttcg gcaacctgcc
    66961 agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa
    67021 catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta
    67081 tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg
    67141 ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc
    67201 ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc
    67261 gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa
    67321 tggttcagca ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg
    67381 ctgtggctga aaaccacgca aaccgtcatt gccgcccagt tcaaccagca cccaacgcgg
    67441 ctgatgctgt ttcagcagag ccggaaggcg cgccagtcct tgttgcgagg tgtcgccgct
    67501 gatgctggca ttaactaccg acgttttact ctgccactta tcattcaaca aggcaggcca
    67561 ggccgcgctg gcagacattc gatacccggc gctcaggcta tcacccagaa tcaataacgt
    67621 gtccgctgcg gcggcacgga aggttaacag gaccaggaac aggaagggca aatgccagcg
    67681 gaaaacattg ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc
    67741 tccatcctca ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc
    67801 gagtcgggat cgggtaagtc aaccttgctg gcgatcctcg ccgggcttga tgacggcagc
    67861 agtggcgaag tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca
    67921 aagttgcgcg cgaagcacgt cggctttgtt tttcagtcat ttatgttaat tcctaccctt
    67981 aacgcgctgg aaaacgtcga gcttccggct ctgctgcgcg gtgagagtag cgcggaaagt
    68041 cgtaacgggg cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct ggatcatctt
    68101 ccggcacagc tttccggcgg tgaacagcaa cgagtggcgc tggcacgagc ctttaatggt
    68161 cgacctgatg tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca gacgggcgat
    68221 aaaattgccg acctgctgtt ttccctcaac cgtgaacatg gcaccacgtt gattatggtg
    68281 acccacgacc tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag
    68341 ttgcaggagg aagcatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat
    68401 taattgtctg gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca
    68461 gcgatcgcat ggagaagggc ttaagccagc aaagccgtga gtttatggcg ggcgatcggg
    68521 cgttgcgcag ttcacgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc
    68581 tgaaagtcgg caagcagctg actttcgcca caatgacctt tgcaggcgac acaccgcagc
    68641 tggcgaacgt caaagcggtg gatgatatct acccgatgta tggcgatctg caaactaatc
    68701 cccctggcct gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc
    68761 ttaacctgaa aacgggcgac accattgacg tgggcgatgc caccttgcgg attgccggag
    68821 aagtgattca ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga
    68881 tgaatctggc ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc
    68941 gttataaatt cggcggcaac gagaaccagc tcgacggcta tgagaaatgg ttgttacctc
    69001 agcttaaacc cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat
    69061 cgatggaacg ctcgcaacag ttcctgctgc tttcggcgct tctgaccttg ctgctggcag
    69121 tggcagcggt ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtggcga
    69181 tcctcaaaac gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt
    69241 tgatggtgct gacgctttca gccgttaccg gtggggccat aggcctgttg ttcgaaaacg
    69301 tgttgatggt gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc
    69361 cgtggctgtg ggcgcttggc accatgacgg tcatctcgct gctggtgggg ctacgaccat
    69421 accgcttgtt gctggcaacg cagcctttac gcgtattacg taatgatgtg gtagcgaacg
    69481 tctggccgct gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg
    69541 gattaatggg tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt
    69601 tgctgtgcgg tgtgctgggc tggatgcagc tgaatgtact tcgccgcatg acgctgaaat
    69661 cgctacctct gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc
    69721 agctttcggc attttcgctc tcctttatgc tgctggcgct gttgctggta ttgcgtggcg
    69781 atctgctcga ccgctggcag cagcagctac cgccagaaag cccgaactac tttttaatta
    69841 acatcgccac agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc
    69901 cggaatcatt ttatccggta gtgcgggcgc ggctgacggc gattaacgat aagccgacag
    69961 aaggtaatga agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc
    70021 ccgatcataa tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg
    70081 aagaggggct ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg
    70141 atacccagga gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc
    70201 ggcctaattt ctattttatt ttcccggaag gggcattaga cgggcaaccg cagagctggc
    70261 ttaccagttt ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc
    70321 cgaccattag cctgttagat attggcgcga ttttaaaaca ggtcggtcag gtgctggagc
    70381 aggtaagtcg ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt
    70441 tgctggcaca ggtgcaggtg ggaatgcgcc agcgtcatca ggagctggtg gtatggcgca
    70501 cactcggtgc ggggaaaaaa ctgctgcgca cgacattgtg gtgtgagttc gccatgcttg
    70561 ggtttgtttc cggcctggtg gccgcaattg gtgcggaaac ggcactggca gtgttgcagg
    70621 cgaaagtgtt tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca
    70681 gcggtgcgct gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta
    70741 aggcgctgtt caggcagttt gcggggtgat gagagtaaat aaggggcgtt ctggttgaat
    70801 ggaacgcctt gtttaaatta attctacaat atgtttcatt gatgatgtta ttgaattggt
    70861 gatttcctat ctttctattg ctgatattaa tctgaatcgg tg
//
LOCUS       DAZKHC020000012        69624 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZKHC020000012 DAZKHC020000000
VERSION     DAZKHC020000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 69624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.012"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(39..299)
                     /gene="yfhL"
                     /locus_tag="ACHOSM_RS04820"
                     /old_locus_tag="pgaptmp_000964"
     CDS             complement(39..299)
                     /gene="yfhL"
                     /locus_tag="ACHOSM_RS04820"
                     /old_locus_tag="pgaptmp_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HGU4557537.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(355..1203)
                     /locus_tag="ACHOSM_RS04825"
                     /old_locus_tag="pgaptmp_000965"
     CDS             complement(355..1203)
                     /locus_tag="ACHOSM_RS04825"
                     /old_locus_tag="pgaptmp_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGU4557538.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLVISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            1412..2047
                     /gene="yfhb"
                     /locus_tag="ACHOSM_RS04830"
                     /old_locus_tag="pgaptmp_000966"
     CDS             1412..2047
                     /gene="yfhb"
                     /locus_tag="ACHOSM_RS04830"
                     /old_locus_tag="pgaptmp_000966"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HGU4557539.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2105..2608
                     /gene="tadA"
                     /locus_tag="ACHOSM_RS04835"
                     /old_locus_tag="pgaptmp_000967"
     CDS             2105..2608
                     /gene="tadA"
                     /locus_tag="ACHOSM_RS04835"
                     /old_locus_tag="pgaptmp_000967"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGU4557540.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQPSTD"
     gene            complement(2605..4161)
                     /gene="mltF"
                     /locus_tag="ACHOSM_RS04840"
                     /old_locus_tag="pgaptmp_000968"
     CDS             complement(2605..4161)
                     /gene="mltF"
                     /locus_tag="ACHOSM_RS04840"
                     /old_locus_tag="pgaptmp_000968"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HGU4557541.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            4419..8306
                     /gene="purL"
                     /locus_tag="ACHOSM_RS04845"
                     /old_locus_tag="pgaptmp_000969"
                     /gene_synonym="purI"
     CDS             4419..8306
                     /gene="purL"
                     /locus_tag="ACHOSM_RS04845"
                     /old_locus_tag="pgaptmp_000969"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGU4557542.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            8864..10291
                     /gene="qseE"
                     /locus_tag="ACHOSM_RS04850"
                     /old_locus_tag="pgaptmp_000970"
     CDS             8864..10291
                     /gene="qseE"
                     /locus_tag="ACHOSM_RS04850"
                     /old_locus_tag="pgaptmp_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HGU4557543.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            10456..11169
                     /gene="qseG"
                     /locus_tag="ACHOSM_RS04855"
                     /old_locus_tag="pgaptmp_000971"
     CDS             10456..11169
                     /gene="qseG"
                     /locus_tag="ACHOSM_RS04855"
                     /old_locus_tag="pgaptmp_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HGU4557544.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            11159..12493
                     /gene="glrR"
                     /locus_tag="ACHOSM_RS04860"
                     /old_locus_tag="pgaptmp_000972"
     CDS             11159..12493
                     /gene="glrR"
                     /locus_tag="ACHOSM_RS04860"
                     /old_locus_tag="pgaptmp_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HGU4557545.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLLMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQVRLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALGGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            12554..12892
                     /gene="glnB"
                     /locus_tag="ACHOSM_RS04865"
                     /old_locus_tag="pgaptmp_000973"
     CDS             12554..12892
                     /gene="glnB"
                     /locus_tag="ACHOSM_RS04865"
                     /old_locus_tag="pgaptmp_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HGU4557546.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(12937..14127)
                     /gene="hmpA"
                     /locus_tag="ACHOSM_RS04870"
                     /old_locus_tag="pgaptmp_000974"
     CDS             complement(12937..14127)
                     /gene="hmpA"
                     /locus_tag="ACHOSM_RS04870"
                     /old_locus_tag="pgaptmp_000974"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HGU4557547.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TSRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDAHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            14455..15708
                     /gene="glyA"
                     /locus_tag="ACHOSM_RS04875"
                     /old_locus_tag="pgaptmp_000975"
     CDS             14455..15708
                     /gene="glyA"
                     /locus_tag="ACHOSM_RS04875"
                     /old_locus_tag="pgaptmp_000975"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGU4557548.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(15771..16964)
                     /locus_tag="ACHOSM_RS04880"
                     /old_locus_tag="pgaptmp_000976"
     CDS             complement(15771..16964)
                     /locus_tag="ACHOSM_RS04880"
                     /old_locus_tag="pgaptmp_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGU4557549.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQTLLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVNYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            17082..20363
                     /locus_tag="ACHOSM_RS04885"
                     /old_locus_tag="pgaptmp_000977"
     CDS             17082..20363
                     /locus_tag="ACHOSM_RS04885"
                     /old_locus_tag="pgaptmp_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HGU4557550.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQESSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADDNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQHSLRTCPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVEALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFIPNQLLRAWQHLDARQPQQACELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQIAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            20460..21443
                     /locus_tag="ACHOSM_RS04890"
                     /old_locus_tag="pgaptmp_000978"
     CDS             20460..21443
                     /locus_tag="ACHOSM_RS04890"
                     /old_locus_tag="pgaptmp_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGU4557551.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAVDKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGGDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            21466..22977
                     /locus_tag="ACHOSM_RS04895"
                     /old_locus_tag="pgaptmp_000979"
     CDS             21466..22977
                     /locus_tag="ACHOSM_RS04895"
                     /old_locus_tag="pgaptmp_000979"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU4557552.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLIGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIALVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            23002..24000
                     /locus_tag="ACHOSM_RS04900"
                     /old_locus_tag="pgaptmp_000980"
     CDS             23002..24000
                     /locus_tag="ACHOSM_RS04900"
                     /old_locus_tag="pgaptmp_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4557553.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            24033..25127
                     /locus_tag="ACHOSM_RS04905"
                     /old_locus_tag="pgaptmp_000981"
     CDS             24033..25127
                     /locus_tag="ACHOSM_RS04905"
                     /old_locus_tag="pgaptmp_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU4557554.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIMAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPHIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFTD"
     gene            25139..26011
                     /locus_tag="ACHOSM_RS04910"
                     /old_locus_tag="pgaptmp_000982"
     CDS             25139..26011
                     /locus_tag="ACHOSM_RS04910"
                     /old_locus_tag="pgaptmp_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HGU4557555.1"
                     /translation="MTIYTLSHGALKLDVSDQGGVIEGFWRDTTPLLRPGKKNGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDQGYAFDFFCLEPMSHASDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(26059..26481)
                     /locus_tag="ACHOSM_RS04915"
                     /old_locus_tag="pgaptmp_000983"
     CDS             complement(26059..26481)
                     /locus_tag="ACHOSM_RS04915"
                     /old_locus_tag="pgaptmp_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU4557556.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(26578..27780)
                     /gene="hcaD"
                     /locus_tag="ACHOSM_RS04920"
                     /old_locus_tag="pgaptmp_000984"
     CDS             complement(26578..27780)
                     /gene="hcaD"
                     /locus_tag="ACHOSM_RS04920"
                     /old_locus_tag="pgaptmp_000984"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HGU4557557.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            complement(27790..28602)
                     /gene="hcaB"
                     /locus_tag="ACHOSM_RS04925"
                     /old_locus_tag="pgaptmp_000985"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(27790..28602)
                     /gene="hcaB"
                     /locus_tag="ACHOSM_RS04925"
                     /old_locus_tag="pgaptmp_000985"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HGU4557558.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERYIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(28599..28919)
                     /locus_tag="ACHOSM_RS04930"
                     /old_locus_tag="pgaptmp_000986"
     CDS             complement(28599..28919)
                     /locus_tag="ACHOSM_RS04930"
                     /old_locus_tag="pgaptmp_000986"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HGU4557559.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(28919..29437)
                     /gene="hcaF"
                     /locus_tag="ACHOSM_RS04935"
                     /old_locus_tag="pgaptmp_000987"
     CDS             complement(28919..29437)
                     /gene="hcaF"
                     /locus_tag="ACHOSM_RS04935"
                     /old_locus_tag="pgaptmp_000987"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HGU4557560.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(29434..30795)
                     /gene="hcaE"
                     /locus_tag="ACHOSM_RS04940"
                     /old_locus_tag="pgaptmp_000988"
     CDS             complement(29434..30795)
                     /gene="hcaE"
                     /locus_tag="ACHOSM_RS04940"
                     /old_locus_tag="pgaptmp_000988"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HGU4557561.1"
                     /translation="MTTPSDLNIYQLIDTQNGRITPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            30931..31821
                     /gene="hcaR"
                     /locus_tag="ACHOSM_RS04945"
                     /old_locus_tag="pgaptmp_000989"
     CDS             30931..31821
                     /gene="hcaR"
                     /locus_tag="ACHOSM_RS04945"
                     /old_locus_tag="pgaptmp_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HGU4557562.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            31981..33120
                     /locus_tag="ACHOSM_RS04950"
                     /old_locus_tag="pgaptmp_000990"
     CDS             31981..33120
                     /locus_tag="ACHOSM_RS04950"
                     /old_locus_tag="pgaptmp_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HGU4557563.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGASLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(33112..34392)
                     /gene="csiE"
                     /locus_tag="ACHOSM_RS04955"
                     /old_locus_tag="pgaptmp_000991"
     CDS             complement(33112..34392)
                     /gene="csiE"
                     /locus_tag="ACHOSM_RS04955"
                     /old_locus_tag="pgaptmp_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HGU4557564.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(34583..35437)
                     /locus_tag="ACHOSM_RS04960"
                     /old_locus_tag="pgaptmp_000992"
     CDS             complement(34583..35437)
                     /locus_tag="ACHOSM_RS04960"
                     /old_locus_tag="pgaptmp_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4557565.1"
                     /translation="MALPVNKRVLKILFILLVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(35582..36385)
                     /gene="suhB"
                     /locus_tag="ACHOSM_RS04965"
                     /old_locus_tag="pgaptmp_000993"
     CDS             complement(35582..36385)
                     /gene="suhB"
                     /locus_tag="ACHOSM_RS04965"
                     /old_locus_tag="pgaptmp_000993"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HGU4557566.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKDSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            36504..37244
                     /gene="trmJ"
                     /locus_tag="ACHOSM_RS04970"
                     /old_locus_tag="pgaptmp_000994"
     CDS             36504..37244
                     /gene="trmJ"
                     /locus_tag="ACHOSM_RS04970"
                     /old_locus_tag="pgaptmp_000994"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HGU4557567.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            37514..38002
                     /gene="iscR"
                     /locus_tag="ACHOSM_RS04975"
                     /old_locus_tag="pgaptmp_000995"
     CDS             37514..38002
                     /gene="iscR"
                     /locus_tag="ACHOSM_RS04975"
                     /old_locus_tag="pgaptmp_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HGU4557568.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            38114..39328
                     /gene="iscS"
                     /locus_tag="ACHOSM_RS04980"
                     /old_locus_tag="pgaptmp_000996"
     CDS             38114..39328
                     /gene="iscS"
                     /locus_tag="ACHOSM_RS04980"
                     /old_locus_tag="pgaptmp_000996"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGU4557569.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            39356..39742
                     /gene="iscU"
                     /locus_tag="ACHOSM_RS04985"
                     /old_locus_tag="pgaptmp_000997"
     CDS             39356..39742
                     /gene="iscU"
                     /locus_tag="ACHOSM_RS04985"
                     /old_locus_tag="pgaptmp_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HGU4557570.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            39759..40082
                     /gene="iscA"
                     /locus_tag="ACHOSM_RS04990"
                     /old_locus_tag="pgaptmp_000998"
     CDS             39759..40082
                     /gene="iscA"
                     /locus_tag="ACHOSM_RS04990"
                     /old_locus_tag="pgaptmp_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HGU4557571.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            40178..40693
                     /gene="hscB"
                     /locus_tag="ACHOSM_RS04995"
                     /old_locus_tag="pgaptmp_000999"
     CDS             40178..40693
                     /gene="hscB"
                     /locus_tag="ACHOSM_RS04995"
                     /old_locus_tag="pgaptmp_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HGU4557572.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLKLREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            40710..42560
                     /gene="hscA"
                     /locus_tag="ACHOSM_RS05000"
                     /old_locus_tag="pgaptmp_001000"
     CDS             40710..42560
                     /gene="hscA"
                     /locus_tag="ACHOSM_RS05000"
                     /old_locus_tag="pgaptmp_001000"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HGU4557573.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNSVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            42562..42897
                     /gene="fdx"
                     /locus_tag="ACHOSM_RS05005"
                     /old_locus_tag="pgaptmp_001001"
     CDS             42562..42897
                     /gene="fdx"
                     /locus_tag="ACHOSM_RS05005"
                     /old_locus_tag="pgaptmp_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HGU4557574.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            42909..43109
                     /gene="iscX"
                     /locus_tag="ACHOSM_RS05010"
                     /old_locus_tag="pgaptmp_001002"
     CDS             42909..43109
                     /gene="iscX"
                     /locus_tag="ACHOSM_RS05010"
                     /old_locus_tag="pgaptmp_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HGU4557575.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            43287..44570
                     /gene="pepB"
                     /locus_tag="ACHOSM_RS05015"
                     /old_locus_tag="pgaptmp_001003"
     CDS             43287..44570
                     /gene="pepB"
                     /locus_tag="ACHOSM_RS05015"
                     /old_locus_tag="pgaptmp_001003"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HGU4557576.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLSIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            44712..45488
                     /gene="sseB"
                     /locus_tag="ACHOSM_RS05020"
                     /old_locus_tag="pgaptmp_001004"
     CDS             44712..45488
                     /gene="sseB"
                     /locus_tag="ACHOSM_RS05020"
                     /old_locus_tag="pgaptmp_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HGU4557577.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(45520..45645)
                     /gene="timP"
                     /locus_tag="ACHOSM_RS05025"
                     /old_locus_tag="pgaptmp_001005"
     CDS             complement(45520..45645)
                     /gene="timP"
                     /locus_tag="ACHOSM_RS05025"
                     /old_locus_tag="pgaptmp_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HGU4557578.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(45981..46826)
                     /gene="sseA"
                     /locus_tag="ACHOSM_RS05030"
                     /old_locus_tag="pgaptmp_001006"
     CDS             complement(45981..46826)
                     /gene="sseA"
                     /locus_tag="ACHOSM_RS05030"
                     /old_locus_tag="pgaptmp_001006"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HGU4557579.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            47033..51994
                     /gene="yfhM"
                     /locus_tag="ACHOSM_RS05035"
                     /old_locus_tag="pgaptmp_001007"
     CDS             47033..51994
                     /gene="yfhM"
                     /locus_tag="ACHOSM_RS05035"
                     /old_locus_tag="pgaptmp_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HGU4557580.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSTVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNITDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            51995..54307
                     /gene="pbpC"
                     /locus_tag="ACHOSM_RS05040"
                     /old_locus_tag="pgaptmp_001008"
     CDS             51995..54307
                     /gene="pbpC"
                     /locus_tag="ACHOSM_RS05040"
                     /old_locus_tag="pgaptmp_001008"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HGU4557581.1"
                     /translation="MLRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESMEEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTSCP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            54456..54887
                     /gene="ndk"
                     /locus_tag="ACHOSM_RS05045"
                     /old_locus_tag="pgaptmp_001009"
     CDS             54456..54887
                     /gene="ndk"
                     /locus_tag="ACHOSM_RS05045"
                     /old_locus_tag="pgaptmp_001009"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGU4557582.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            55037..56191
                     /locus_tag="ACHOSM_RS05050"
                     /old_locus_tag="pgaptmp_001010"
     CDS             55037..56191
                     /locus_tag="ACHOSM_RS05050"
                     /old_locus_tag="pgaptmp_001010"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HGU4557583.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            56476..57489
                     /gene="rodZ"
                     /locus_tag="ACHOSM_RS05055"
                     /old_locus_tag="pgaptmp_001011"
     CDS             56476..57489
                     /gene="rodZ"
                     /locus_tag="ACHOSM_RS05055"
                     /old_locus_tag="pgaptmp_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGU4557584.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            57516..58634
                     /gene="ispG"
                     /locus_tag="ACHOSM_RS05060"
                     /old_locus_tag="pgaptmp_001012"
                     /gene_synonym="gcpE"
     CDS             57516..58634
                     /gene="ispG"
                     /locus_tag="ACHOSM_RS05060"
                     /old_locus_tag="pgaptmp_001012"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HGU4557585.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            58745..60019
                     /gene="hisS"
                     /locus_tag="ACHOSM_RS05065"
                     /old_locus_tag="pgaptmp_001013"
     CDS             58745..60019
                     /gene="hisS"
                     /locus_tag="ACHOSM_RS05065"
                     /old_locus_tag="pgaptmp_001013"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGU4557586.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELQGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            60037..60657
                     /gene="yfgM"
                     /locus_tag="ACHOSM_RS05070"
                     /old_locus_tag="pgaptmp_001014"
     CDS             60037..60657
                     /gene="yfgM"
                     /locus_tag="ACHOSM_RS05070"
                     /old_locus_tag="pgaptmp_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HGU4557587.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            60669..61847
                     /gene="bamB"
                     /locus_tag="ACHOSM_RS05075"
                     /old_locus_tag="pgaptmp_001015"
     CDS             60669..61847
                     /gene="bamB"
                     /locus_tag="ACHOSM_RS05075"
                     /old_locus_tag="pgaptmp_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HGU4557588.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            61965..63437
                     /gene="der"
                     /locus_tag="ACHOSM_RS05080"
                     /old_locus_tag="pgaptmp_001016"
     CDS             61965..63437
                     /gene="der"
                     /locus_tag="ACHOSM_RS05080"
                     /old_locus_tag="pgaptmp_001016"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGU4557589.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            63506..63721
                     /locus_tag="ACHOSM_RS05085"
                     /old_locus_tag="pgaptmp_001017"
     CDS             63506..63721
                     /locus_tag="ACHOSM_RS05085"
                     /old_locus_tag="pgaptmp_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGU4557590.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIELKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(63718..65088)
                     /gene="xseA"
                     /locus_tag="ACHOSM_RS05090"
                     /old_locus_tag="pgaptmp_001018"
     CDS             complement(63718..65088)
                     /gene="xseA"
                     /locus_tag="ACHOSM_RS05090"
                     /old_locus_tag="pgaptmp_001018"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGU4557591.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIISPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHLLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            65250..66716
                     /gene="guaB"
                     /locus_tag="ACHOSM_RS05095"
                     /old_locus_tag="pgaptmp_001019"
     CDS             65250..66716
                     /gene="guaB"
                     /locus_tag="ACHOSM_RS05095"
                     /old_locus_tag="pgaptmp_001019"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGU4557592.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            66785..68362
                     /gene="guaA"
                     /locus_tag="ACHOSM_RS05100"
                     /old_locus_tag="pgaptmp_001020"
     CDS             66785..68362
                     /gene="guaA"
                     /locus_tag="ACHOSM_RS05100"
                     /old_locus_tag="pgaptmp_001020"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGU4557593.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(68457..68996)
                     /locus_tag="ACHOSM_RS05105"
                     /old_locus_tag="pgaptmp_001021"
     CDS             complement(68457..68996)
                     /locus_tag="ACHOSM_RS05105"
                     /old_locus_tag="pgaptmp_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HGU4557594.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQKQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(69012..69530)
                     /locus_tag="ACHOSM_RS05110"
                     /old_locus_tag="pgaptmp_001022"
     CDS             complement(69012..69530)
                     /locus_tag="ACHOSM_RS05110"
                     /old_locus_tag="pgaptmp_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU4557595.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT        16364 a        17554 c        19185 g        16521 t
ORIGIN      
        1 cagtaatata attaatactc tacttccaga gtagaatatt aaattttatc cgcgtggtgc
       61 atcagcacaa atttatccca caactgttct tctgtctcga catgcgccgg atctttcaca
      121 atagtattgg ggatcgggca caccttctgg caggttggtg tctcgtagtg ccctacgcat
      181 tcggtacact tatcgctgtt aatctcgtag atatgatctc ccattgaaat cgcctcattc
      241 gggcattcgg gttcacacat atcacaattg atgcattttt tagtgattaa caacgccata
      301 aggaaactct caaaacaaca caaaccgggc gggcattata cgcgcattct ttactcagac
      361 cagttttttc accagcgctt cactatgacg aatacgttct ggtgccagtt ccagatcctg
      421 ctgaatcagc gcaatgaaca gcaaatccgt taacattccc tgagcgtgac aagcagagat
      481 tgaagcactg tttgtcgctt gttcttcggc aatggtatac aggcaatgag aagcacgctg
      541 ttgcagggca ttcggagtaa agccggtaat cgccagcact tttccgccca ctcgcagcat
      601 ctcatctgcc gccaggttta actcgcgtcg tacaccggtg taggaaatga ctaataacag
      661 atcgtcaggg gacgacgcct gtactgttgc gagtagcgca tgcatatcgc gcactgcggc
      721 agcattgaag ccaatcttca tcagcttcca ggcaaagttt tgcgccacca gacccgaagc
      781 gccaataccg gtcagaatta tccgccgcgc agagcgcaac attgttacgc attcatgcag
      841 tttctcttca ctattaacgt ttagcgttgc gtacatagcg gcagtatttt ctttaatcag
      901 tttttcgccg accagccgta acggatcatc accgcggatt tggttatgaa tgggcacgga
      961 gggcgattcc ggctggcttg ccagcgcttc actcaacgcc aacttaagcg ccggaaaacc
     1021 tttatagccg agtttttgcg cgaacttcac gacgctggac tgactgactc cggcttcgtt
     1081 ggccagttgc tgagagctta aatggcgcgc cgtatcaggt tgtagcagca gataatccgc
     1141 cagtttttta tcactctggg caagcccctg gtaacgctga cggatacgaa gtaagccgtt
     1201 catatctttc ctcgcgatga aatagtggcg acaacaggca atatgagaca cacgcgagtg
     1261 aattttatat tccattgccg ctaattgcca tgaattaaaa attccagtta gccatgatgc
     1321 ggtcgattgt tgccggaaaa gtgatgatct atctgttagg ctatgtagta gcgaaaatta
     1381 atcaggatgt ttcagtccag aggagtatgg tttggcaact cacgagcgtc gtgtggtgtt
     1441 ttttgactta gatggaacat tgcatcagca ggatatgttc ggcagttttc tgcgctattt
     1501 actgcgtcgc caaccgctga atgcgttact tgtcctgccg ttgttaccga ttatagccat
     1561 tgcgttattg ataaaaggtc gtgcggcacg ctggccgatg agtctgcttc tgtgggggtg
     1621 cacttttggt cacagcgaag cacgtttaca gacgttgcag gccgatttcg tgcgctggtt
     1681 tcgcgacaat gttaccgcct ttccgctggt tcaggagcga ttaaccacct acctgttaag
     1741 ttccgatgct gatatctggt tgattaccgg ctctccgcag ccgctggttg aagcggttta
     1801 tttcgatacg ccctggctgc cgcgggttaa tcttatcgcc agccaaattc agcgtggcta
     1861 tggtggttgg gtattgacga tgcgttgtct gggacatgaa aaggtcgcac aactggagcg
     1921 caaaatcggc actccgctgc ggctgtacag tggctatagc gacagtaatc aggacaatcc
     1981 gctgctttat ttctgtcagc atcgttggcg agtaaccccg cgcggtgaac tccagcaact
     2041 ggaatagagt aaagcatagc gtccgtgtat aatgcgccgc gcttttataa ccggagtttt
     2101 ctttttgtct gaagtcgaat ttagccacga atactggatg cgtcacgcgc tgacgctggc
     2161 gaaacgtgcc tgggatgagc gggaagtgcc ggtcggcgcg gtattagtgc ataacaatcg
     2221 ggtaatcggc gaaggctgga accgcccgat tggtcgccat gatcccaccg cacatgcaga
     2281 aatcatggcc ctgcggcagg gtggtctggt gatgcaaaat tatcgtctga tcgacgccac
     2341 gttgtatgtc acgcttgaac catgtgtaat gtgtgccgga gcgatgatcc acagtcgcat
     2401 tggtcgcgtg gtctttggtg cgcgtgacgc gaaaactggc gctgcgggat ctttaatgga
     2461 tgtgctgcat catccgggta tgaatcaccg agtggaaatt acggaaggaa tactggcgga
     2521 tgagtgcgcg gcgttgctca gtgacttctt tcgcatgcgc cgccaggaaa ttaaagcgca
     2581 gaaaaaagca caaccctcga cggattaatt ttgtttctct tcactccctt tcctggaaaa
     2641 cagcagagag ggagaatggg tcaaatagtt tgatgacgac tgggaaagaa acgagagaaa
     2701 aggaaatttc tctttgccca actccgtagg cgataccgcc ggataatcct gcgccagttg
     2761 catcgccgct tctgtagcct gcttctcttt ctcttgcaga taacccacca ggctaatctg
     2821 atacttacga atattttcga cataagcgta ggcttcatgt ccacgagcgt agccgtaagt
     2881 cagcttgctg taatagggtt tctggctaag taaaggcaga cgctgtttta cgtcagccca
     2941 actgtcagga ttccctttgg tttttgccgt cagggcgcgg gcatccagca tatgcgcata
     3001 gcccatattg tacgcagcga gggcaaacca gatccgctcg ttctccggca cactttccgg
     3061 cactttactc atcatatcct gcaaataacg cacgccaccg ctgatgctct gttcggcatc
     3121 ggtacgatcc gtaatgccga ggctttgcgc ggtatttttg gttaacatca tcatgccgcg
     3181 cacacccgtc ggtgaagtgg cctgtgcatc ccagtgcgat tcctgataag caatagcggc
     3241 cagcaaacgc cagtcaattt cttcggcgta tttctcaaac aggggcttta actgcggcag
     3301 taccgcatcg acggcgcgta aaaatgtgcg cgtatcgacg taatcaaaat catcgccatg
     3361 ccccaggtat ttctcttcaa tgcgtgccag cgtaccgtct tcattcattt cgttgaagaa
     3421 gtcgagcagg gcggcggaaa gggtattatc gccatctaac gggctaaacc aagtcaccgg
     3481 ttgttcatcg gtgatatcga gcgctacggc gagctccggg tgaacgcgct gaaacaggct
     3541 gatggcgaca gaatcagcaa tggtgtaatc gagttttcct tcgatgacat cttccattaa
     3601 ttccgcagag ccttttttgt cgtctacctt ccagcttaat tccgggaatt ttgtttcttt
     3661 cagggtctgg agatcgttaa ccaccacatg acccggtgca acggtgagtt gctccgccgt
     3721 caggttgccc agcgtacgtg ggcgatactg acccacttta taaaccagtt gttgtgacac
     3781 ggaataatag gtagggccag gctgataatt ttttacccgc tcactgttat agaccagtcc
     3841 tgccgccagc aggtcggcgt taccattatc aaggtcgtca aacagctggc tgatattctg
     3901 ccgcacggtc actttcagtt ttacgccgag gtaatcggca aactgtttcg ccagttcgta
     3961 atccaggcca aaaggtttcc cgttgatttc gttataagtc aggggagtat gaatggtgct
     4021 cacacgcaac tctccccgcg cttgaatggc ggcgatacgg ttgtcggctt taccaaacca
     4081 gggaatggat ggccagagag cgaccgcgag cagcagtgcc agaatgccga tgaacagata
     4141 attaatcttt aattttttca attagttaat tctctgtgtc gtgcgcgtcc cagcttgaaa
     4201 aaacgtaata atagtgaaag gtttactcat aaatgagcgg cattttgcgt aaacctgcgc
     4261 cagatggcaa cttattacag ccattggcgg cacgcgttgc taattcacga tggtgatttt
     4321 atttccacgc aaacggtttc gtcagcgcat cagattcttt ataatgacgc ccgtttcccc
     4381 cccttgggta caccgaaagc ttagaagacg agagacttat gatggaaatt ctgcgtggtt
     4441 cgcctgcact gtcggcattc cgaatcaaca aactgctggc acgttttcag gctgccaggc
     4501 tcccggttca caatatttac gccgagtatg tccattttgc tgacctcaat gcgccgttaa
     4561 acgatgatga gcacgcacaa cttgaacgcc tgctgaaata tggcccggca ctcgccagcc
     4621 acgccccgca aggcaaactc ctgctggtga ccccgcgtcc tggcaccatc tctccctggt
     4681 cttcgaaagc gaccgatatt gcccataact gcgggctaca acaggtaaac cgccttgagc
     4741 gcggcgttgc ttactatata gaagccggta cgctgaccaa tgaacaatgg cagcaggtta
     4801 ccgctgaact gcacgaccgc atgatggaaa cggtcttttt tgctttagat gatgcagagc
     4861 agttgtttgc ccaccatcaa ccgactccgg ttaccagcgt tgatttgctg gggcagggcc
     4921 gtcaggcgct gatcgacgct aacctgcgtc ttggcttggc tctggcggaa gatgaaattg
     4981 actatctgca ggatgctttc acaaagcttg gtcgtaaccc gaacgacatc gagctgtata
     5041 tgttcgctca ggcgaactct gagcactgcc gccacaaaat ttttaatgcc gactggatta
     5101 tcgacggtga acagcagccg aaatcgctgt tcaggatgat caaaaatact ttcgaaacca
     5161 cgccagatca cgttctctct gcttataaag ataacgccgc cgtaatggaa ggttctgaag
     5221 tgggccgcta ctttgctgac cacgaaacgg gccgctacga tttccatcag gaaccggcgc
     5281 atattctgat gaaagtcgaa actcacaacc acccgacggc gatttctccg tggccgggcg
     5341 cggcgacggg ttccggcggc gaaattcgtg atgaaggcgc caccggacgc ggtgcgaagc
     5401 cgaaggctgg tctggttggt ttctccgttt ccaacctgcg aattcctggc ttcgaacagc
     5461 cgtgggaaga agatttcggt aagcctgagc gcattgtcac cgcgctggac atcatgaccg
     5521 aaggcccgct gggcggcgcg gcgtttaaca acgaatttgg tcgtccggca ctgaatggct
     5581 acttccgtac ttatgaagaa aaagtgaaca gccacaacgg cgaagagctg cgcggttatc
     5641 acaaaccgat catgctggcg ggcgggatcg gcaacattcg cgccgatcac gtacaaaaag
     5701 gcgagatcaa cgtcggtgcg aagctggtcg ttctcggcgg cccggcaatg aacatcggtc
     5761 ttggcggcgg agcggcgtct tccatggcgt caggtcagtc tgatgccgac ctcgactttg
     5821 cttccgtaca gcgcgacaac ccggagatgg agcgtcgctg ccaggaagtg atcgaccgtt
     5881 gctggcagct tggtgatgcc aacccaatcc tgtttatcca cgacgtcggc gctggcggtc
     5941 tttctaacgc gatgccggaa ctggtgagcg acggcgggcg cggcggtaaa tttgaactgc
     6001 gcgatattct aagcgacgaa ccgggcatga gcccgctgga aatctggtgt aacgaatccc
     6061 aggaacgcta cgtgctggcg gttgctgccg atcaattacc gctgtttgac gaactgtgta
     6121 agcgtgagcg cgcaccctac gcggtgattg gtgaagcgac cgaagaactg catctttctc
     6181 tgcacgatcg tcattttgat aatcagccga tcgatctgcc gctggacgtc ctgcttggta
     6241 aaacgccgaa gatgacccgc gatgtacaaa cgctgaaagc gaagggcgat gcgctggtcc
     6301 gtgaagggat caccattgca gacgcggtga aacgcgtact gcatctgccg accgtggcgg
     6361 agaaaacttt cctggtgacc atcggtgacc gcagcgtaac tggcatggta gcgcgcgatc
     6421 agatggtggg tccgtggcag gtgccggtcg ctaactgcgc ggtcactacc gccagcctcg
     6481 acagctacta cggtgaagcg atggcgattg gcgagcgtgc gccggttgcg ctgctggatt
     6541 tcgccgcctc tgcccgtctg gcggtcggtg aagcgttaac caatattgcc gcaacacaga
     6601 ttggcgatat caaacgcatt aaactttccg ccaactggat ggcggcggca ggccaccctg
     6661 gtgaagatgc gggcctgtat gaagccgtta aagccgtggg cgaagagctt tgtccggcgc
     6721 tgggcctgac gatcccggtg ggtaaagact ctatgtcgat gaaaacccgc tggcaggaag
     6781 gtaacgaaga gcgcgaaatg acgtcgccgc tgtcgctggt gatttctgca tttgcccgcg
     6841 tggaagatgt gcgtcacacc atcacgccac agctttctac cgaagataac gcactgctgc
     6901 tgattgattt gggcaaaggc aataacgcgc tgggcgcaac ggcgctggcg caggtttatc
     6961 gtcagcttgg cgacaaaccg gcagatgtac gcgacgtcgc gcaactgaaa ggcttctatg
     7021 acgcgattca ggcgctggtt gcacagcgta agctgctcgc ataccacgac cgctctgatg
     7081 gcggcctgct ggtaacgctg gcggaaatgg cctttgctgg tcattgcggc attaacgcgg
     7141 atattgcctc tctgggcgac gatcgcctgg cggcgttgtt taacgaagaa ctgggtgcgg
     7201 tgattcaggt tcgtgccgct gaccgtgaag cggtcgagtc cgtactggca cagcatgggc
     7261 ttgctgattg tgtccattat gtagggcagg cggtttccgg tgaccgtttt gtgattaccg
     7321 ccaacgggca gactgtattc agcgaaagcc gcaccacgtt gcgtgtctgg tgggcagaaa
     7381 ctacctggca gatgcagcgc ctgcgtgaca acccggagtg tgccgatcag gagcatcagg
     7441 cgaaatctaa cgacgccgat ccgggcctga atgtaaaact gtcgttcgat atcaacgaag
     7501 atgtggcagc accgtatatt gccactggcg cacgtccgaa agttgccgta ctgcgtgagc
     7561 agggcgtgaa ctcgcatgtt gaaatggcgg cagctttcca ccgtgcaggc tttgatgcta
     7621 tcgacgtgca tatgagtgac ctgctgaccg gacgcacggg cctggaagat ttccacgccc
     7681 tggtcgcgtg cggtggtttc tcctacggtg atgtgctggg tgccggtgaa ggttgggcga
     7741 agtcaatcct gttcaatgac cgtgtacgcg atgagtttgc aaccttcttc caccgtccgc
     7801 aaacgctggc gctgggggta tgtaacggtt gccagatgat gtctaacctg cgcgaactga
     7861 tcccaggtag tgagttgtgg ccacgttttg tgcgcaatac ctccgatcgc tttgaagcgc
     7921 gtttcagcct ggttgaagta acccaaagcc cgtctctgct gttgcagggg atggtgggct
     7981 cgcaaatgcc gattgctgtc tctcatggtg aagggcgcgt ggaagtgcgt gatgcggcgc
     8041 atctggcggc actggaaagc aaagggctgg tggcactgcg ctatgtcgat aacttcggca
     8101 aagtcactga aacctacccg gctaacccga acggttcacc gaacggtatt acggcagtca
     8161 cgactgaaag tggtcgagtc accattatga tgccgcaccc ggaacgtgtt ttccgtactg
     8221 tcagcaactc ctggcatccg gaaaactggg gcgaggatgg cccatggatg cgcattttcc
     8281 gcaatgcgcg taagcagttg gggtaattca tcagttcatt ggttttgcag cccggatgcg
     8341 gcattaagca ccaaccacta tcataagctc ccaactccgg gagctttttt gtgtctgtaa
     8401 atcacgacaa tgggtggttt accgtgtcgc tttctggcga cacttaaatg attgatttta
     8461 ttgtaatcta ataagtttat cttaagttgg ctctttttag cgacacagtg gctgatttac
     8521 attcaaatag cgaagaagcc ataaatccct caaagcaacg tataatcagt acgttaccaa
     8581 actattttct ttattggcac agttactgca taatagtaag cagtggctca ttcaccgact
     8641 tatgtcagcc ccttcgggac gtgctacata aaattcgaat gacgcacaac aaggtgcctg
     8701 ccgtccaact tctgatatca gcgtagctat atcaaccatc gggcgaaacg tcgagttagg
     8761 caccgcctta ttccataaca aagccgggta attcccggct ttgttgtatc tgaacttccc
     8821 ctcggttagc atcaggctat tcgcgtctga cgagagtaac accttgaaac gctggcccgt
     8881 ttttccccgc tcattacgac aactggtaat gctggcattt ttgctaattc tactacccct
     8941 gttggtgctg gcatggcaag cctggcaaag cctgaatgcg cttagcgatc aggcggcgct
     9001 ggttaaccgc actacgctta ttgatgcccg gcgtagtgaa gcgatgacca acgcggcgct
     9061 ggagatggag cgtagctacc gtcagtattg cgtgctggac gaccctacgc ttgcgaaggt
     9121 ttatcaaagc cagcgcaagc gttacagcga aatgctcgat gcccacgcag gcgtactgcc
     9181 agacgataaa ctttaccagg cattgcgtca ggatttgaac aatctggccc aacttcagtg
     9241 taacaacagt ggtcccgatg ctgccgccgc cgcgcgtctt gaagcctttg ccagtgccaa
     9301 taccgaaatg gtgcaggcca cgcgcacagt ggtgttctct cgtgggcagc aacttcagcg
     9361 tgaaatcgcc gaacgtgggc aatattttgg ttggcaatcg ctggtgctat ttctggtgag
     9421 tctggtgatg gtgctacttt tcacgcggat gattatcggg ccggtgaaaa atatcgagcg
     9481 catgatcaac cgtctggggg aagggcgttc tctgggcaat agcgtttcgt tcagtggacc
     9541 gagcgagtta cgctcggttg ggcaacgtat tctttggtta agtgagcgcc tgtcatggct
     9601 ggaatcccaa cgccatcaat ttttaagaca tttatctcat gaattaaaaa cgccactggc
     9661 gagtatgcgc gagggcactg aattactggc tgaccaggtt gtcgggccgc ttacgccaga
     9721 gcaaaaagag gtggtgagca ttcttgatag cagcagccgc aatttgcaaa aactgatcga
     9781 acaactgctt gattacaacc gtaaacaggc ggacagtgcg gtggaactgg agaatgttga
     9841 attagcaccg ctggtggaga cagtggtttc tgctcatagc ctgcccgcac gggctaaaat
     9901 gatgcatacc gacgtcgatc tcaaagcaac agcttgcctg gcggagccaa tgctgctgat
     9961 gagcgtactg gataatcttt actccaatgc ggtgcactac ggggctgaat ccggtaacat
    10021 ttgccttcgc agcagttcac atggtgcgcg ggtttatatt gatgtcatca atacaggcac
    10081 gcccattccg caagaggaac gcgccatgat cttcgaaccc ttttttcagg gaagccacca
    10141 gcgaaaaggg gcggtgaagg gcagcggtct gggattaagc attgccaggg attgtattcg
    10201 ccgtatgcaa ggggaactgt atctggtcga cgagagcggg caagacgttt gtttccgcat
    10261 tgaattaccg tcgtcgaaaa acacgaaata atgctaacag cctatcctat caggctattt
    10321 tatttgtcag ttcggccccg ggcagtgctc gaaatcttca cgtacgatat gtacgctccg
    10381 gtttctccgc gctgtccatg tccagactgt ctgcaacaat tacgcccact gggataggct
    10441 ttaagtctgg tgaatatgcg acacattttt caacgattat tgccccgacg gctgtggctg
    10501 gcagggctgc catgtctggc attgctgggt tgcgtgcaga atcacaataa gccagccatt
    10561 gatacgccag cagaagaaaa aattccggtt tatcaactgg ctgactatct ttctactgaa
    10621 tgtagcgaca tctgggcgct gcaaggtaaa tcaaccgaaa ccaatccgct ttactggctg
    10681 cgggcgatgg attgtgctga tcgtttaatg cctgcgcagt cgcgtcagca agctcgccaa
    10741 tatgacgacg gcagctggca aaataccttt aaacaaggga ttttgctcgc cgacgccaaa
    10801 attacgccgt atgagcgccg ccagctggtc gcgcgtattg aagcgttaag taccgagatc
    10861 ccggcacagg ttcgcccact gtatcaactc tggcgcgacg gtcaggcgct gcaactgcaa
    10921 ctggcggaag agcgccaacg ttacagtaaa ctccagcagt ccagcgacag cgagctggat
    10981 acattgcgcc agcaacatca cgttctacag cagcaactgg aactcaccac ccgcaagctg
    11041 gaaaacctga ccgatattga acgccagctc tcgacccgca aaccggctgg aaatttctcg
    11101 ccggatacgc cgcacgagag tgaaaagccc gcgccatcca cccatgaggt cactcctgat
    11161 gagccataaa cctgcgcatt tattattggt cgatgacgat ccgggattgc tgaaactgct
    11221 tggcctgcgc ctgaccagcg aaggctacag tgtggtcacg gcggaaagtg gcgctgaagg
    11281 attacgggta ctgaatcgcg aaaaagtaga tttagttatc agcgacctgc tgatggatga
    11341 aatggacggt atgcagctat ttgctgaaat ccagaaagtg cagccgggaa tgccagtaat
    11401 tattcttact gcgcatggtt ctatccccga tgccgttgct gcaacacagc agggcgtttt
    11461 tagtttcctc accaagcctg tcgacaaaga cgcgctatat caggcaattg acgatgcgct
    11521 ggagcaatcc gcgccagcca ccgatgaacg ctggcgcgag gcaattgtca cccgcagccc
    11581 gctgatgctg cgtttgctgg aacaggtgcg gctggtggcg caatcagacg tcagcgtttt
    11641 gattaacggt cagagcggca ccgggaaaga gattttcgcc caggctatcc acaacgccag
    11701 cccgcgcaac agcaaaccat ttattgctat taactgtggc gcattgcccg agcaattgct
    11761 ggagtcggag ctgtttggtc atgcgcgtgg cgcgtttact ggcgctgtca gcaatcgcga
    11821 aggtttattc caggcggcgg aaggcggaac gctatttctc gatgaaattg gcgatatgcc
    11881 tgcgccgtta caggtcaaac tgctgcgcgt gttgcaggag cgtaaagtgc gcccgctggg
    11941 cagtaaccgc gatattgata tcaatgtgcg gattatttct gccactcacc gtgatctgcc
    12001 aaaagcgatg gcgcgcgggg aattccgcga agatctctat taccgcctca acgttgtcag
    12061 cctgaaaatt ccggcactgg cggagcgcac agaagacatt ccactactgg caaatcacct
    12121 gttgcgccag gcggcagagc gacataaacc gtttgtccgc gcgttctcta ccgatgcgat
    12181 gaaacgcctg atgaccgcga gctggccggg taatgtgcgc cagttggtca acgtgattga
    12241 acagtgcgtg gcgctgacct catctccggt gattagtgat gcgctggtgg agcaggcgct
    12301 ggggggtgaa aatacggcgc tgccaacctt tgttgaggca cgtaatcagt ttgaactcaa
    12361 ctatttgcgt aagctgctgc aaatcaccaa aggcaacgtc acccacgcgg cgagaatggc
    12421 ggggcgcaac cggacagaat tttataaact gctttcccga cacgagctgg atgcaaacga
    12481 tttcaaggaa tgaattggcg ttatgtgtta cgtttagcag atcaaaagac aggcgacctt
    12541 ttcaaggaat agcatgaaaa agattgatgc gattataaaa cccttcaagc tggacgatgt
    12601 ccgcgaagca ctggccgaag tcggtattac cggcatgacg gtgaccgaag tgaaaggctt
    12661 tggtcgccag aaaggccata ccgagctgta ccgcggcgcg gagtatatgg tggattttct
    12721 gccgaaagtg aaaattgaga ttgtcgtacc ggacgacatt gtcgatacct gtgtcgatac
    12781 cattattcgc acggcgcaaa ccggcaaaat cggtgacggt aaaatcttcg tctttgacgt
    12841 ggcacgggtc attcgcatcc gtaccggtga agaggacgac gcggcaattt aaaccgcgtc
    12901 aagggttgcc ggatgtttcc atccggcaac atcaaattac agcaccttat gcgggccaaa
    12961 gcattcgtaa tgaatgtttt cctgcttcac gcccagatcc actaactgtt tcgcggtaaa
    13021 ctgcatgaag ccaaccgggc cgcagagata gaactgcatt gtcggatcgc tgaacgcacc
    13081 ttccagtttg ctcaaatcca tcagaccttc gctatcaaac tgacctttag cgcgatcggc
    13141 ttcgctcggc tgacgatacc aggtgtgcgc ggtaaagcgc ggcagtgact gccccagttc
    13201 cttaacttca tcagcaaagg cgtgagcatc gccattttct gccgcatgga accagttcac
    13261 ttgtgctgtg tggcctgctt ttgccagcgt gtcgagcatt gccagcattg gcgtttgacc
    13321 aacaccggca gagattaacg tcactggtgt gtcatctgcg acagccataa agaaatcacc
    13381 tgccggagcg accagtttca cgacatcgcc aacattggcg tgattgtgca accagttgga
    13441 tacctgccca ccctcttcgc gtttcaccgc aatacgatag cctttgccat ccggtttgcg
    13501 agtcaaagag tactgacgaa tttcctgatg cgggaaacct tccggcttca gccagacgcc
    13561 gagatattgc cccggacggt attctgccac tgcgccaccg tcgaccggct ccagttcgaa
    13621 gctggtgata agcgcgctgc gcgatgtttt agccacaatg cggaaatcgc gagtaccttc
    13681 ccaaccaccg gctttgctgg cgttttcgtt atagatttcc gcctcgcgat tgataaatac
    13741 attagccagt acaccatagg ctttacccca agcgtccagc acttcctgcc ccgggctgaa
    13801 catttcgtcc agcgtcgcca acaggtgttc gccgacgatg ttgtactgtt ccggtttgat
    13861 ctggaaactg gtgtgcttct gcgcgatttt ttctaccgct ggcagcagcg caggcaggtt
    13921 ttcaatatta ctggcgtagg cggcaatagc gttaaacagg gcttcacgtt gatcgccatt
    13981 acgctggtta ctcatgttaa aaatttcttt gagttctggg ttatgagtaa acatacggtc
    14041 gtagaaatgg gcggttaact ttggccccgt ttccaccagt aaagggatgg tggcttttac
    14101 tgtagcgatg gtttgagcgt caagcatatg gtcttccttt ttttgcatct taattgatgt
    14161 atctcaaatg catcttataa aaaatagccc tgcaatgtaa atggttcttt ggtgtttttc
    14221 agaaagaatg tgatgaagtg aaaaatttgc atcacaaacc tgaaaagaaa tccgtttccg
    14281 gttgcaagct ctttattctc caaagccttg cgtagcctga aggtaatcgt ttgcgtaaat
    14341 tcctttgtca agacctgtta tcacacaatg attcggttat actgttcgcc gttgtccaac
    14401 aggaccgcct ataaaggcca aaaattttat tgttagctga gtcaggagat gcggatgtta
    14461 aagcgtgaaa tgaacattgc cgattatgat gccgaactgt ggcaggctat ggagcaggaa
    14521 aaagtacgtc aggaagagca catcgaactg atcgcctccg aaaactacac cagcccgcgc
    14581 gtaatgcagg cgcagggttc tcagctgacc aacaaatatg ctgaaggtta tccgggcaaa
    14641 cgctactacg gcggttgcga gtatgttgat atcgttgaac aactggcgat cgatcgtgcg
    14701 aaagaactgt tcggcgctga ctacgctaac gtccagccgc actccggctc ccaggctaac
    14761 tttgcggtct acaccgcgct gctggaacca ggtgataccg ttctgggtat gaacctggcg
    14821 catggcggtc acctgactca cggttctccg gttaacttct ccggtaaact gtacaacatc
    14881 gttccttacg gtatcgatgc taccggtcat atcgactacg ccgatctgga aaaacaagcc
    14941 aaagaacaca agccgaaaat gattatcggt ggtttctctg catattccgg cgtggttgac
    15001 tgggcgaaaa tgcgtgaaat cgctgacagc atcggtgctt acctgttcgt tgatatggcg
    15061 cacgttgcgg gcctggttgc tgctggcgtc tacccgaacc cggttcctca tgctcacgtt
    15121 gttactacca ccactcacaa aaccctggcg ggtccgcgcg gtggcctgat cctggcgaaa
    15181 ggtggtagcg aagagctgta caaaaaactg aactctgccg ttttccctgg tggtcagggc
    15241 ggtccgttga tgcacgtaat cgctggtaaa gcggttgctc tgaaagaagc gatggagcct
    15301 gagttcaaaa cttaccagca gcaggtcgct aaaaacgcta aagcgatggt agaagtgttc
    15361 ctggaacgcg gctacaaagt ggtttccggc ggcactgata accacctgtt cctggttgat
    15421 ctggttgata aaaacctgac cggtaaagaa gcagacgccg ctctgggccg tgctaacatc
    15481 accgtcaaca aaaacagcgt accgaacgat ccgaagagcc cgtttgtgac ctccggtatt
    15541 cgtgtaggta ctccggcgat tacccgtcgc ggctttaaag aagccgaagc gaaagaactg
    15601 gctggctgga tgtgtgacgt gctggacagc atcaatgatg aagccgttat cgagcgcatc
    15661 aaaggtaaag ttctcgacat ctgcgcacgt tacccggttt acgcataagc gaaacggtta
    15721 atctgttgaa aaaggcttcc ttcgggaagc ctttttacgt tatcgcgcca tcaaatctgt
    15781 cgtaactgcg cctcaacata caaatatcca atccccagca cctgttgggc gcggcttaat
    15841 tgcccaaagc caatttgcgt cgctttcacg ctcggtcctt cgtcacggtc gaacgggtta
    15901 aatcctgtct ggcgaataat ggtattaagc cagtgctcac caaaggcaca actgcgaccg
    15961 tacaaccaaa tctgattaat attgaggatg ttgaggaagt tatacagact caaaccaatg
    16021 gcattggcac tgcggtcaac ccagctggtg atccacggtt ctccactctg ccaggcagcg
    16081 attaactgcg cggttgtcag tttttcagga tcaagctgag tattaaccgg ttgtgatttt
    16141 agccataccc gcgcctgttt ttttaatgca cttaacgagg cgacggtttc caggcagccg
    16201 taacgtccac agtcgcagac gacgccatcc ggattaacga tggtgtgacc aatctgtccg
    16261 ctaccataca aactgccgcg ataaatttgc tcgttaatca cgaacgacga gccaatgccg
    16321 taattaacgt tgatcacgca gaaatcccgt tcctgcgaat tattttgcca tttctccgcc
    16381 agcgccagca tcacgcagtc attatcgacc atcacccgaa tgccgagctt ctcttccagc
    16441 agatacttca cctcaactgg cgttgtccac ggcgcttgcg gcatggtttg cgacacgccg
    16501 gtcacaggat caacctgacc gtggattgcc agcgccagat tgatggtgcg gtccggccac
    16561 aatttacggt ggcgatgcca gcatttttcg atttcggaca gcagcgtctg cggcgtcggt
    16621 gcatcaatct gtaaatactc aaattcacct ttcggactta aacaagcatt agcaacctga
    16681 cactcaatac tggtgggcgt cacgttcagg cacagcgtcc agtcaccttc cggcgcaatc
    16741 agccatgtac cgctactatg cccgcgcgtc tggctttcat cgtcgatatt caccacccgt
    16801 ttttcgcttt ccagttcctg caaaatatta ctgactgccg gaatcgaaat ttgcgccagc
    16861 cgggccagcg ttgatttatt ggcgcgcttt tgccggtaca gcagttccag aatcacgcat
    16921 ttgttatggt ggcgaatctg ttgattgtta atgcaggctc tcatcttcac taaaccttgt
    16981 ttagttaatt gcgtgattat tccgcgaaaa cattgccaga ttgttcaata cactgccaca
    17041 aatcttttaa ttcagtatgt cttgttaata ttgagggcac catgactcca gtaaaagtgt
    17101 ggcaagagcg cgttgagatc ccgacctatg aaaccgggcc gcaggatata catcccatgt
    17161 tcctcgaaaa tcgcgtttat caggaatcgt ccggcgcggt ttatccctac ggcgtgaccg
    17221 atacgctgag cgagcagaaa accctgaaat cctggcaggc ggtgtggctg gaaaacgact
    17281 acatcaaagt gatgatcctg ccggaactgg gcggtcgggt gcatcgcgcg tgggataaag
    17341 tgaaacagcg cgattttgtt tatcacaatg aagtcattaa acctgcgctg gtggggctgc
    17401 tggggccgtg gatttccggt gggattgagt ttaactggcc gcaacatcat cgcccgacca
    17461 cctttatgcc cgttgatttc accctcgaag cccatgaaga cggtgcacag acggtgtggg
    17521 taggcgaaac ggagccgatg catggtttac aggtgatgac aggtttcacc ctgcgccctg
    17581 accgggcggc gctggaaatc gccagccgcg tctataacgg taacgccacg ccgcgtcatt
    17641 tcttgtggtg ggccaacccg gcagtgaaag ggggggaagg gcatcagagc gttttcccac
    17701 cggatgtgac tgcggtgttt gatcacggca aacgggccgt ctccgctttc cccatcgcca
    17761 ccggcactta ctacaaagtg gactactccg ccggagtgga catttctcgc tataaaaatg
    17821 tgcctgttcc aacctcatat atggccgaaa aatcgcagta cgattttgtc ggcgcgtggt
    17881 gtcacgatga agatggcggt ttgctgcacg ttgccaacca ccatattgcg ccaggtaaaa
    17941 aacagtggag ttggggacac agtgaatttg gccaggcgtg ggataagagt ctgaccgaca
    18001 ataacggccc gtatatcgaa ctgatgaccg gtatttttgc cgataaccag cccgatttta
    18061 cctggcttga tgcttacgaa gagaagcgtt ttgagcagta tttcctgcct tatcattctt
    18121 tgggcatggt gcaaaatgcc tcccgcgatg cggtgataaa actccagcgt agtaagcggg
    18181 ggattgagtg ggggctgtat gccatctctc cgttgaacgg ataccgcctg gcgatccgcg
    18241 aaatcggcaa atgcaacgcg ttacttgatg atgccgtggc actgatgcct gcgaccgcca
    18301 tccagggcgt gttgcacggt atcaatcctg aaaggctgac cattgagctc tccgatgccg
    18361 acgacaatat tgtactgagt tatcaggaac atcagccgca agagttgccg ttgccggacg
    18421 tcgccaaagc gccactggca gcacaagaca ttaccagtac agatgaagcc tggtttatcg
    18481 gtcagcatct ggagcaatat catcacgcca gccgttcacc gttcgattac tacctgcgcg
    18541 gcgtggcgct ggatccgctg gattaccgct gtaacctggc gctggcgatg ctggaatata
    18601 accgcgccga tttcccgcaa gcggtggcgt atgccactca ggctctgaaa cgcgcacatg
    18661 cgctgaacaa aaatccgcag tgcggacagg ccagtttgat tcgcgccagt gcttacgaac
    18721 gtcagggaca atatcaacaa gccgaagagg atttctggcg tgcggtctgg agcggcaata
    18781 gtaaagcagg cggctattat ggtctggcac gactggcggc gcgtaatgga aacttcgacg
    18841 cgggtctgga tttttgccaa cacagtcttc gcacctgccc aaccaatcag gaagtgcttt
    18901 gcctgcataa tctgctgctg gtgttaagtg gtcgtcagga caacgcgcgt ttgcagcgcg
    18961 agaaactgct gcgcgattat ccgctgaacg ccactctgtg gtggctgaac tggttcgatg
    19021 gtcgtagcga atcagctctc gcgcagtggc gcggtctgtg tcagggacgc gacgttaacg
    19081 ccctgatgac cgccgggcaa ctgattaact ggggaatgcc caccctggcg gcagagatgc
    19141 tgaacgcact ggactgccag cgcacgctgc cgctttacct gcaagccagc ttgctgccga
    19201 aagccgaacg tggcgaactg gtcgcaaaag ccattgatgt cttcccgcag tttgtccgtt
    19261 tcccgaatac gctggaagaa gtggaggcgc tggagagtat tgaagagtgc tggtttgctc
    19321 gccatttact ggcctgcttc tactacaaca aacgtagcta caacaaagcc attgccttat
    19381 ggcaacgttg cgtagagatg tcgccggagt ttgccgacgg ctggcgcggg ttagcgatcc
    19441 atgcgtggaa taagcaacac gattatgagc tggccgcgcg ttatcttgat aatgcttatc
    19501 agcttgcgcc gcaggatgca cgtctgcttt tcgaacggga tttgctggat aagttaagcg
    19561 gagccacacc ggagaaacga ctggcgcgtc tggaaaataa tctggaaatt gcgctgaaac
    19621 gcgacgacat gaccgcagaa ctgctcaatt tgtggcatct cacgggtcag gcagacaaag
    19681 cggcggacat tctcgccacg cgcaaattcc acccgtggga aggcggggaa gggaaggtca
    19741 ccagtcagtt tatccccaac cagttattac gcgcctggca gcatcttgat gccagacagc
    19801 cgcagcaggc ctgcgaactg cttcatgccg cgctgcatta tccggagaat ttaagcgaag
    19861 gccgtttacc ggggcaaact gataacgaca tctggttctg gcaggcgata tgcgccaacg
    19921 cgcagggcga tgaaactgaa gcgacgcgtt gtttacgtct ggcggcgacc ggcgatcgca
    19981 ccattaacat ccacagttat tacaacgatc agccggttga ttatctcttc tggcaaggaa
    20041 tggcgctgcg actgctgggc gaacaacaaa tcgcacagca actgtttagt gaaatgaaac
    20101 agtgggcgca agagatggcg aaaaccagta ttgaggcgga tttctttgct gtttcacaac
    20161 ctgacctgtt gtcgctgtat ggcgatttac aacagcagca taaagaaaaa tgcctgatgg
    20221 tggcgatgct ggcgtccgcg ggactcgggg aggttgcgca atatgaatct gctcgcgctg
    20281 aattgacggc gattaatccg gcctggccga aagcggcatt attcaccacc gtgatgcctt
    20341 ttatttttaa ctacgttcac taaaccctat taactgtaat atttgagcgg cacgcgctgc
    20401 cgctcatcac aaaataatta gaataagccg ttgttaataa aaaccctaca ggaggcacta
    20461 tgcctaaaaa aatgagaaca acccgtaatc tattattaat ggctacgcta ttaggtagcg
    20521 cactatttgc cagggctgtg gataaagaaa tgaccattgg tgcaatatac cttgataccc
    20581 agggatatta cgctggagtg cgtcagggcg ttcaggatgc ggcaaaagat tcttcagtac
    20641 aggtacagtt aattgaaact aacgcccagg gtgatatttc gaaagaaagt acctttgttg
    20701 ataccctcgt ggcgcgtaat gtcgatgcca ttattttatc ggcagtgtct gaaaatggca
    20761 gtagccgtac cgttcgtcgc gccagtgaag cgggtattcc ggtgatttgc tacaacacct
    20821 gtattaatca aaaggggggc gataaatatg tctcggcgta tctggtcggc gatccgctgg
    20881 aatttggtaa aaaactgggt aacgctgccg ccgattattt tattgccaat aaaattgacc
    20941 agccgaaaat tgccgtcatc aattgcgaag cctttgaagt ttgtgtgcag cgacgtaaag
    21001 gatttgaaga agtattaaaa tcccgcgttc ccggcgcgca aattgtcgct aatcaggaag
    21061 ggactgtttt agataaagcg atttccgttg gtgaaaaact gattatctcc acgccggatc
    21121 tcaacgccat tatgggggag tcgggcggtg cgacactcgg cgcggtaaaa gcggtacgta
    21181 atcaaaatca ggccggaaaa attgctgttt tcggttcgga tatgacaacc gaaattgctc
    21241 aggagctgga aaacaatcag gtgctgaaag cggtagtgga tatttccggt aagaaaatgg
    21301 gcaatgctgt tttcgcgcaa acattgaagg ttatcaataa acaagccgac ggtgaaaaag
    21361 tgattcaggt gccaatcgat ctctatacca aaacggaaga tggtaaacag tggctggcaa
    21421 cgcacgttga tggtctgccc taatcgctga ttcgcagagg tccctatgtt cacggcaaca
    21481 gaggcagtcc cggtagcaaa agtggtggca ggaaataagc gttaccccgg cgtcgttgcg
    21541 ctggataacg ttaacttcac gctcaataaa ggcgaagttc gtgcgctgtt aggtaaaaac
    21601 ggcgcgggca aatctactct cattcgaatg cttatcggca gcgaacgccc ggatagcggt
    21661 gatatctgga ttggcgagac gcgactggaa ggtgacgaag ctacgttgac tcgccgtgcc
    21721 gctgaactgg gggttcgtgc ggtttatcag gaattaagtc tggtggaagg gctgacggtg
    21781 gcagaaaacc tctgccttgg tcaatggcct cgccgcaacg gcatgattga ttacctgcaa
    21841 atggcgcagg atgcccaacg ttgcttacag gcgctgggcg ttgacgttag tcctgaacaa
    21901 cttgtttcaa cgctaagccc ggcgcaaaag caactggtgg aaattgcgcg ggtgatgaag
    21961 ggcgagccgc gcgtggtcat tcttgatgaa cccaccagct cgcttgccag tgcagaagtt
    22021 gaactggtga tcagtgcggt gaaaaagatg tcggcactgg gcgtggcggt gatttatgtc
    22081 agccaccgga tggaagaaat ccgccgcatt gcctcctgtg ccaccgttat gcgcgatggt
    22141 caggtggcgg gcgatgtgat gctcgaaaac acctccacgc atcatattgt gtcgctaatg
    22201 ctagggcgtg atcacgttga tattgcgctg gttgcccctc aggaaattgt ggatcaggcc
    22261 gtactggaag tccgtgcgtt acgccataag cccaagctgg aggatatcag ctttacgtta
    22321 cgtcgcggcg aagtgcttgg cattgctggc ctgctggggg cagggcgcag tgaattgctg
    22381 aaggcgattg ttgggctgga gacgtatgaa cagggcgaaa ttgttatcaa cggcgagaaa
    22441 atcacgcgcc ccgattacgg cgacatgctg aaacgcggca ttggctatac gccagaaaac
    22501 cgcaaagaag cggggatcat tccctggttg ggcgttgacg aaaatacagt gctgaccaat
    22561 cggcaaaaaa tcagcgccaa cggagtgctg caatggtcca ccatccgccg cctgaccgaa
    22621 gaggtgatgc agcggatgac ggtcaaggcc gccagtagcg aaacgcccat cggcacgctt
    22681 tctggtggca atcagcaaaa agtggtgatc ggtcgttggg tctatgccgc cagccagatt
    22741 ttgttgctcg acgagccaac gcgcggcgtc gatatcgaag ccaaacagca gatttaccgt
    22801 attgtccgtg agctggctgc cgaaggaaaa agcgtggtgt ttatctccag tgaagtggag
    22861 gagttgccgc tggtgtgtga ccgcatcctg ttattacagc acggcacgtt ctcgcaggag
    22921 tttcactcgc cggtcaatgt ggatgagctg atgtccgcca ttctgtctgt gcactgatga
    22981 attttaccga ggaacctgaa gatgtctgct tcgtcattac cattgccgca gggcaagagc
    23041 gtctcgctca aacaatttgt cagtcgccat attaatgaga tcggtttgct ggtggtgatt
    23101 gccattcttt atctggtctt ctccctgaac gcgcctggct ttatctcatt gaataaccag
    23161 atgaacgtgc tgcgcgatgc cgccaccatt gggattgccg cctgggcgat gacgctgatt
    23221 attatctccg gtgaaattga tgtcagcgtt gggccgatgg tggcttttgt ctcggtgtgc
    23281 ctggcatttt tgctgcaatt tgaagttccg ttggcgattg cttgtctgct ggtgttgctg
    23341 ttaggcgcgc tgatgggaac gctcgccggg gtgctgcgcg gcgtgtttaa cgtaccaagt
    23401 ttcgttgcca cactggggct gtggagcgcc ctgcgcggaa tggggctgtt tatgaccaac
    23461 gcactgccag tgccgattga cgaaaacgag gtgctggact ggctgggcgg acaatttctc
    23521 ggtgtgccag tatccgcgct gatcatgatg gtgttgtttg cgctgtttgt gttcattagc
    23581 cgaaaaaccg ccttcgggcg ctcggttttt gccgttggcg gtaatgccac ggcggcgcag
    23641 ttgtgcggta ttaacgttcg tcgggtgcgc attcttatct ttaccctttc gggattatta
    23701 gcggcggtga ccggcatttt gctggcggca cgcctcggtt ccggtaacgc aggtgccgca
    23761 aacggtctgg agtttgacgt catcgccgcg gtcgtggtcg gcggtacggc actttccggc
    23821 ggtcgcggtt ccctgttcgg tacattgctt ggtgtgctgg tgattacgct aatcggtaac
    23881 ggtctggtgc tgctcggtat taactccttt ttccagcagg tggtacgcgg cgtcatcatc
    23941 gtggtggcgg tgctggcgaa tatcttgctg acccagcgaa gcagtaaagc gaaacgctaa
    24001 accttaacgc ccctggcgcg ggtgcggcag ggatgaaaat taatcgtatt gttaatgagg
    24061 gatttatgaa aacgatgctg gcagcttatt taccaggaaa ttcaaccgtc gatctgcggg
    24121 aagttgcggt gccgacgccg gggattaacc aggtactgat caaaatgaaa tcctccggga
    24181 tttgcggaag cgatgtccac tatatctatc atcaacaccg tgccacggcg gcggcacccg
    24241 ataaaccgtt ataccaggga tttatcaacg gtcatgaacc gtgcgggcag attatggcga
    24301 tggggcaagg ctgccgccat tttaaagagg gcgaccgcgt gctggtgtat cacatttctg
    24361 gctgtggttt ttgcccgaac tgccgccgcg gctttcctat ctcttgtact ggcaaaggaa
    24421 aagcggctta cggctggcag cgtgacggcg gtcatgccga atatctgctg gcggaagaaa
    24481 aagatctgat cctcctgccg gatgcgctga gctacgaaga tggtgcgttt atcagttgcg
    24541 gcgttggcac ggcctatgaa gggattttgc gcggcgaagt ttccggcagc gataacgtgc
    24601 tggtggtcgg tctggggccg gtcggcatga tggcgatgat gctggcgaaa ggtcgcggtg
    24661 caaaacggat catcggcgtt gatatgctgc cggaacgtct ggcgatggca aaacagttag
    24721 gggtgatgga tcacggctat ttagcaacca ccgaaggtct gccgcatatt atcgccgaac
    24781 tcacccacgg tggcgcggat gttgcgctcg attgttccgg taatgccgca ggtcgcttgc
    24841 tggcactgca atccaccgct gactggggac gggtggttta cattggtgaa accggaaaag
    24901 tggaattcga ggtcagcgcc gatctgatgc accatcaacg gcggattatc ggctcctggg
    24961 tgaccagtct gttccatatg gaaaaatgcg cccacgattt gactgactgg aagctgtggc
    25021 cgcgtaacgc cattacccat cgcttctcgc tggaacaggc aggtgatgcc tatgcgctga
    25081 tggcgagcgg caaatgcggg aaagttgtga ttaacttcac ggattaagga gttagcggat
    25141 gaccatctat accttatccc acggggcgct aaagctggac gtttctgacc agggcggcgt
    25201 aatcgaaggc ttctggcgcg atacgacgcc gctcttacgt cctggtaaaa aaaacggtgt
    25261 ggcaaccgat gcctcatgtt ttccgctggt gccatttgca aaccgggtga gcggcaaccg
    25321 ttttgtctgg caggggcgtg agtatcaact gcaaccgaat gttgagtggg atgcgcacta
    25381 tctacacggc gatggctggc tgggcgagtg gcaatgtgtc tcgcacagcg atgatagttt
    25441 gtgtctggtg tatgaacatc gcagcggtgt ctatcactat cgggtaagtc aggcgtttca
    25501 tttaacggcg gatacactga cggtgacgct ctctgtcacc aatcaagggg cagagacgct
    25561 gccatttggt accggctggc atccttattt tccgttgtca ccacaaacgc ggattcaggc
    25621 gcaggcgagc ggttactggc tggaacggga gcagtggctg gcgggtgagt tttgcgagca
    25681 gctaccgcag gaactggatt ttaaccagct cgcgccgttg ccgcgccagt gggtgaacaa
    25741 tggttttgcc ggatggaatg gtcaggcccg catcgagcag ccgcaggaag gttatgccat
    25801 catcatggaa acgacgccac ctgcaccgtg ttatttcatc tttgtttccg accctgcgtt
    25861 tgatcaagga tatgcgtttg atttcttctg tctggaaccg atgagccatg cgtcggatga
    25921 tcatcatcgc ccggaagggg gtgatctcat tgcgctagca ccaggggaat caacaacttc
    25981 agagatgtcg ttgcgggtag agtggctgta atttattaaa tgccggataa ggcgttcacg
    26041 ccgcatccgg catctttcct aacgccgatc gagagaaatt gcccccggcc cggtaattgc
    26101 caatagcaag aacgcgccag cgatactgac attcttccag aaattaatca tatttggccc
    26161 aaccgcatcg ccggtcatat cccagtaatg gtgaccaatc accgccgtac ccagcgtgta
    26221 gaaaataaac agcaccgcca gcggacgggt gaaaaagcca agcacgatta atatcgcggc
    26281 gggcacttcc ataactaccg caataatcgc tgccagcatc ggcattggcg cgcccaacga
    26341 ggccatatat tggaccgtac cgtcaaagcc catcattttg ggaaaaccaa aaataatgaa
    26401 aattaagacc acggcgatac gggcaattaa taacaaaacg gggcgggcag ctccaaaatc
    26461 aaaataacgt agtgtgttca taaccaatcc ctttgcaaga taatgtgatt taaacgtaat
    26521 acacactctt gcacctcgcc acgccccagg cgttgagata ttcgctaatt atcctggtta
    26581 cagtgattta agcgcgatgt tctcatctat cagcagtttc gcatcaaacg ttttgccgct
    26641 ctggatccat ttgcgaattg ggcgaatctc acgcccctga ttcagcgtga ccgcaccgat
    26701 aagcacgccg ttttgcagat taaaccaaat cgccttctga gtttccgggt tgccacgaca
    26761 aagccagtca tcgccacgca tatcgccaat aaactgtaag ttatcactgt actgatcgct
    26821 ccagaaccac ggcggcggca gtagcggtag cggtagccct aacattgcgg cagcggcaat
    26881 ttgcgcctgg ttattggcgt tttcccagct ttcgcagcgg tgtagtgcac cattatcaag
    26941 acgagtgatt gccacatcgc caccggcaaa gatcgcggga tcgcaggtgc ggcaagcctc
    27001 atcaatgaca atgccattgg cagtatcaag gttggcctcg cgagccagtt gctcgttggc
    27061 gctgatacca ataccgtaaa tcaccacatc agcctgaagc gtttccccac tttgcagcgt
    27121 cagttctact ttttcaccat cgaccacatg ttcaatggca ttattgagca gaatgcgcac
    27181 accagcctgc tggtggcgct gtaaaagata gcgttgcacg ggcggtggtg cattacggcc
    27241 catgacggtt gccgccagtt caatcactgt caccttacat ctgcgctgcg tggcgctggc
    27301 agccagttcc agaccaatag ttccggcacc gacaatcacg actgaccgtt cgggctgcag
    27361 aacttctcgc agtctggcgg catcaccggc atggcgtagg gtaaagcagc gttctcccag
    27421 tgcatcaagc aacggcagcg gtcgagctgc cgcgccggtt gctataaaaa gctgatccca
    27481 gtgccagctt tcgccgttgg ttaacactaa ctctcgtgtg tcgcgaccca gcgttttgat
    27541 ggttacaccg gaatgcagat gaacattgtt ttcctgccac cagttagcgg gtaacacctg
    27601 ctgtaactgc ggggaatctt ccagcaacat ggatttcgag agtggaggtc gttcataagg
    27661 aagatgtcgc tcatcggaaa acagatgcag ctcaccggtg aacccttgct ggcgtagcga
    27721 ggccgcagcc attgccgccg cttgcccgcc accgacaata atgatcgttt tttctttcat
    27781 cgtgcttcct taaagatcca gcccagccgc tacgtggcga atgccgcgaa tcgctaaacc
    27841 cgcatcagcg ttgatcatca caccgcttaa tgcgcgatta ttgcgccgcg atgccaacat
    27901 cacatacggc cccgtaaaat ccgccggttg cgggaaaaat tgcagcggta aaatggcggc
    27961 aattttctcc ggcgtcagag actgcattat cgaggtttca ctttgcccga gcgcctgtgg
    28021 gccgcgcagg tcgctggcca taccacacgg gccgacgcca ttcacccgca ctttcggtgc
    28081 cagttcataa gccagttggc gaataagtcc ggttgcggca tgtttactgg cggtgtacag
    28141 cgggccaccg ccgccaggat accaggcggc atttgacagt gtgaaaatca tgctgccttc
    28201 actggcgatt aacgccggag cgcaggcttt tgcgcccagc aggtaaccga gtacgttgac
    28261 gttaaacagc tcgtggaagc cggtttcgag cgtctctgcg ggagtattaa ccagtgaggc
    28321 attgtgatcc cagatgcctg cattgccgat aaaacaatcc agcttgccgg aacgagtcag
    28381 gatctgatcg accgcgcgtt gataatcggc ataacaggtt acgttacctt ccaccgccag
    28441 aatatgttcg ccaaatcgct gacgcagact ggcgactttt gccgccgaca gttccagcgt
    28501 ggcaacctgc gcgccttctt cgatatatcg ctcgaccagc gccagcccta atcccgatcc
    28561 gccgccggta ataaaaatgg actcgttatg cagatcgctc atggctgcgc ctccggtaag
    28621 tcgatgaaaa tgtcaccacc ttcaacgtgt actggataag tggtgagcgg atcggtggcg
    28681 ggcaggcata acgctttccc cgttttcagg caaaaactgg cggcgtgtag cgggcactcc
    28741 accgtggcgt catcttccag atacccttct gacatcgacg cattaccatg gctgcaacga
    28801 tcgttaatgg cataaaactc gccgccaacg ttgaacaggg cgatgacggg ggaggtatcg
    28861 atccggagag cctcaccctc cggcacatcc gcaacgggac acgcataaat tcgattcatc
    28921 agaacagtac actcaggtta tgggaagtga ttaccgcttg atccaggacg atatcccgtt
    28981 ccagcaagcg ccagttgtca tcttccagac ggcgaacttt gtcgaaacgc gttccaacat
    29041 agaatgtttc atcgcgctct ttttgtgccc gataaagtag ataattcacc cgcacagcaa
    29101 atacgtttgg gatgtcggtt tcgcttatct ggcagttgct gattaagtga cgggtgcgtg
    29161 acggcggctc ttctgcccag gccatgcccg tttccagacg ggcgattcgc cgctccagct
    29221 ggtctttggt gtcattaaaa atccaggttg tcggtggctg gacgcctttg cggcggtcgc
    29281 gtgtttgcgc gttaactgtg gtgcgcatgg tgtaacgaat ctcttcgtcg agctgcgcca
    29341 gccagtcacg aaatttccag tcgtccagta agctggcttc gtgaaagaga aactggctaa
    29401 tgcggtgatg taactctagt gaaacttgcg cactcatttc atcacctcct gctggtaagc
    29461 ggcggtttta tcgagcactt cctgccagct ttcgctactc agaagatcgg cccagcgttg
    29521 gtacattcca cgagcggcag tttctgaaaa gatatagtta gtaatgccag gaatgccgtc
    29581 gtcgcggcgc ttttcctgac caagccccat ttccagacac agtttgctgt tgcgggcgcg
    29641 gtggccttta agcaatttct ggatttcaca ccagttctcc gagtcatcct gctcgagaaa
    29701 accagcagga ccaaaagcac gagtggcgct gttttcaaaa gcggctttaa cttcatcgga
    29761 ggcggctttg tcagtaatac agaacgccca cacttcaact tgatcagggc cgcgcggatg
    29821 ccagacgcgg agcgtggcag tgccgttgag ccatgaaagc gtggggaaaa tattgttatg
    29881 acccgccagg cgcagggcgc gaacttcacc taaacgttgt tctgcttcgg cataggtttc
    29941 gcggtaatag cttgaaactg cgccatcgac ccagacatta gcatccggtt tttcagtaaa
    30001 gaagaaaccg ctaccgtgac cgtcctgacc aaattgcagc gcatctttgg cggtttccca
    30061 caccgggcgg gcggtttgtc catcaccgag gcgcttatcg ctgccatcat ctttcgcccc
    30121 taatacctga acggcagaag catggctgaa cagagcatga tactggtcac tggcgaactg
    30181 ctctgccggg aatttccagt tacagttgat cacccacttt tgtacgccgc cgacaatttc
    30241 ggtgccgcct tcgcgacgat ccagcatgcc atccagatac caggcaatgt caccaaggta
    30301 atcacgcagg cccggtgcgc tggtatccca gttgccaaaa atcagccctt tataactctc
    30361 cacacaagga acttcgttta gcccccagtg ggatttacac aacccttgtg ggtaggcgcg
    30421 aggttccagc ggtacatcga tcaactcgcc gttaatgcca taagaccagc cgtgatacgg
    30481 gcaggtaaag gcgcgagtgt tgccgcaatc tgcataactc acacgcatgg cccggtggcg
    30541 gcattggttg agaaacgcct tgatgctgcc gtctttctga cgcactacga caaccgcatc
    30601 ttctcccatg taggtgttaa agaaatcacc gggttttggg atctggcttt cgtgggcgag
    30661 aaatagccag caacgaccga aaatacgctc aagctccagt tggtaaatat ccgggtcggt
    30721 ataaatacgc ggagtgatcc gaccattttg ggtgtcaatc agttggtaaa tgttcaaatc
    30781 tgagggtgtg gtcatatttt ttaatcctga aagataagat gtattgacct tctatttttg
    30841 tgccataagg atgttgctgg atggttgcta atatgtgaaa tagttaattt ctaccagctg
    30901 agacgttttt tcgataacaa ggggaaggtg atggaactac ggcatttacg ctatttcgtc
    30961 gcagtggcgc aggcactgaa ctttacccgt gcggcggaaa aactgcatac ctcacagcct
    31021 tcgttaagca gccagatccg cgatcttgaa aactgtgtcg gtgttccgtt actggtgagg
    31081 gataagcgca aagtcgcgtt gacggcggcg ggagaatgtt ttctccagga cgcgctggca
    31141 atcctcgaac aagcggaaaa tgccaaatta cgggcgcgga aaattgttca ggaagacaga
    31201 caattaacca ttggctttgt gccatcggcg gaagtgaatt tattgccaaa agtattaccg
    31261 atgtttcgtc tcagacagcc agacaccttg attgagctgg tgagtttaat caccacgcaa
    31321 caggaggaaa aaattcgccg tggtgaactc gacgtcggct taatgcgcca tcctgtttat
    31381 agcccggaga ttgattatct ggagcttttt gacgaaccat tagtggtggt gttaccggtt
    31441 gatcaccctt tagcacatga aaaagagatc accgccgcgc aactggatgg cgtgaatttc
    31501 gtcagtaccg atccggcgta ttccggttcg cttgcgccga tcgttaaagc gtggtttgcg
    31561 caagaaaaca gccagccaaa tatcgtccag gtggcaacga atattctggt gaccatgaat
    31621 ctggtgggca tggggctggg cgtcactttg atacccggtt atatgaataa ttttaatacc
    31681 gggcaggttg tttttcgtcc cattgccgga aatgtgcctt ccattgcttt actgatggcg
    31741 tggaagaaag gggagatgaa gccggcatta cgcgatttca tcgccattgt gcaggaacgt
    31801 ttggcaagcg taacggcata attacgcatt atttttgcaa catgcgtcgc ggatctcagt
    31861 tttttagtgc ttgtgaactg gcgttacaga catcgggaag caggctcgaa tattcttatt
    31921 tttcaacttg caccaacatt cgattaaccc cagactaacg cctcctgacg ggagggactc
    31981 atggttttgc aatccacgcg ctggttggcg ctcggctatt tcacatactt ttttagttac
    32041 ggcatttttc tacctttctg gagcgtctgg cttaaaggga ttggtttaac gccagaaacc
    32101 atcggcctgt tattgggggc aagtctggtt gcccgttttc tcgggagttt gctcatcgcg
    32161 ccccgcgtca gcgatccttc ccgcctgatt tccgccttgc gcgtgctggc actgctgaca
    32221 cttctctttg ctgtcgcctt ctgggcgggg gcgcacgtag cgtggctgat gctggtgatg
    32281 attggcttta acctcttttt ctcaccgctg gtaccgttga ccgatgcact ggcgaatacg
    32341 tggcaaaagc agttcccgct tgattacggc aaagtgcgac tgtggggctc ggtggcgttt
    32401 gtcattggct cggcgctgac gggcaaactg gtcactatgt ttgattatcg ggtgatcctc
    32461 gcgctgttga cgttgggcgt ggcatccatg ctgctcggct ttctcatccg tccgacgatt
    32521 cagccacaag gggcaagccg ccagcaggag agcaccggtt ggtcagcgtg gttggcgctg
    32581 gttcgccaga actggcgctt tctggcctgc gtttgtttat tgcagggggc acatgcggcc
    32641 tattacggtt ttagcgccat ttactggcag gcagctggct actcggcctc ggcggtgggg
    32701 tatttgtggt cgctgggcgt ggtggcggaa gtcattatct ttgcgctgag taataaactt
    32761 ttccgccgtt gtagtgcacg cgatatgctg ttgatctcgg cgatttgcgg cgtagtgcgc
    32821 tggggcatta tgggagcaac tacggcgttg ccgtggttga tagtggtgca aattctgcat
    32881 tgcggcacct tcacggtctg ccacctggcc gccatgcgtt atattgctgc tcgccagggt
    32941 agcgaagtca tccgtttaca ggcggtttac tctgccgtcg cgatgggcgg cagtatcgct
    33001 atcatgaccg ttttcgccgg tttcctgtat caatatctgg gccacggcgt gttctgggta
    33061 atggcgctgg tggcgctgcc ggcaatgttt ttgcgcccga aagttgttcc ctcatgctga
    33121 ttcgagcatt ttgcggatct gctcctgttg atgtgttgtc agcgtcaggt ccgtatagat
    33181 cagcggtggt gaaaagagcg gtaatggcat ggtataaggc gcaataatca gtgccgcgcc
    33241 acgcggagcg cctgtctgca aaaatgcctt taccgacata tgcttaatgt tgagcggcag
    33301 taacgttagt tcgcgtaact gctgctcaat ctgcgcttct cgctcgctat cattcccggt
    33361 cagtagaata atctgttttt catgcagatc gttttcctgc attagccagg caccgaaaat
    33421 caccgcgacc agaccacttt cctcatcaga aagatggacg ccgtattcac tttcaaatcc
    33481 ggccagcgcc gcgcgggtgg tgcgtaccag gcgtggatac agtctggcga actcttccgg
    33541 cagggtatta tcaataccga tggcaaacaa actgcgattt aacgcctggg cgaggtgggt
    33601 ataaagctga tcacataacc cctgttcatc ataaaaacga acatttccca gctcacgaaa
    33661 atggtttacc agacgtttga tagactgacg caattgtctg tcccgctgat gcgcatcccg
    33721 caatggatcg ggaacccgca acatggaaaa aagtagtgcc ataaacagtg gctcatcagg
    33781 tggtacaggc tggagcgccc gacgctgcca gtggcgtcca atttcctgcg ctacctgaaa
    33841 ttcaaggcag gattccgccc agcgacgttg gagcggatta aactgaggcg taattccagc
    33901 gtggtgctgc agcaaacaat attgcagata cagacacagg aaatgaatat cgcgcgattc
    33961 aaagcgtttt tgcagccgtc gggagcagag attcactaac gcttgtagat tggtatcgtc
    34021 gtaaaagtta cgcgcaattc cgcgccgctt cagttcactc ttcagggcgg gggtgaaatg
    34081 gctggtaata aatgatggac acagacggaa accacgtcgt agccagtgaa ataaacacaa
    34141 acgttggtta agcactgtac cttcaacccg gtagctaccg tcataaccag ccgtcagtgt
    34201 gagttgatga tagcgcagga tctcctgtcc tgtttcgctg atatcaagac tggcaatatc
    34261 atcatccaca ccattaagct cgctgaaagt tgccgtggtg gcggtcaacc ccggctgaaa
    34321 gagcgtcagc aagatctggc agcggcgctg gggagccgaa aggacagatg gtggagcaag
    34381 cgtaggcatc atctgtagat ctcccataag cataattgat aagaatagct aaagtttgct
    34441 cagggaaatg acgaagggaa tgctaatcat cagaaatgtt ggcaagcgtc acagaatttt
    34501 tctcatctta aactgttctg accagtggat tagcatgtct taaggtggtt ttgtgaatga
    34561 ttatcgtgct agcgatcatg ggttagtttt gcggattcaa cgcgctaagg ataaagtcca
    34621 ccatctgttc gcgataaaca tcgccgtggc gatcggaaaa agcatcaatg tgttcgccat
    34681 ccgggattag gatcaaccgt tttggctctt ttgccaggct atacaacttt tcgctgtgct
    34741 gccatgggat aacgtgatca gctttaccgt gaatgagtaa aagcgggatc gggctgacgc
    34801 tggcgatata attttcgccg ctgtaactct catcaagtaa gtagccactg ccggggatca
    34861 tctggttggc gatggttgca taagaggcaa atgtggagtc gaggatcacc gcacgtatgc
    34921 cttcacgatc accctgacca ataacagcaa gaatattcgc cccgccaata ctctgcccga
    34981 acagcaccag ccgttgtggg tttacatcac tgcgatggcg caccacattg atggcgcttt
    35041 gcgtatcgtc cagcaatccg gcctgggacg gcgtgccttt tgatttacca aacccgcgat
    35101 aatcaaacat aaaaacgttg aaattacgct cgggtaacca actgaccagc ggccagtggg
    35161 cggacatatt tccggcattg ccgtgagcat gaatgatggt tgcgatggcg ttgtcagcag
    35221 ggcccgtcga agaaggaata aaccagcctt gcagacgagt accgtcctta gccgtaaatt
    35281 cgacggattc cgccgaccag gggtcgggac cgtaaatttt atcgtcagga taatagaaga
    35341 agttgatggc aacgcgcggc actaaataaa cgcagaaggc aacaacaagg agaataaaca
    35401 gaattttgag aacgcgtttg ttcactggca gtgccataac ctttccttgc gacttcttta
    35461 tcatccatta atcggggaaa gatggtggga gagtgacgaa agaaagtaaa gcggtgagga
    35521 agtatttcgg aatagtgcct gaaaggcggg ggcaggtgag tgatatcacc cgcctgagtc
    35581 attaacgctt cagagcgtcg cttaactcgt cacgcatgtt cgccagcatg gctttcacaa
    35641 cgcgcgggtt accagcaacg atgttaccgg tcagcatgta gttatgacca ccggtgaagt
    35701 cgctgacgat gccgcccgct tcacgaacca gcagctcgcc tgccgcgaaa tcccacggac
    35761 gcagaccgat ttcaaagaaa ccgtcaacac gacccgctgc gacgtaagcc agatccagcg
    35821 ccgcagaacc ggtacgacgg aagtctgcac attcgttgaa tagtttgccg acgatgttga
    35881 tgtaggtagt ggcgtactgt ttggctttga acgggaagcc ggtcgccaga atagtaccgt
    35941 cgagatcgcg agcggtgctg ccgcgcagac ggtagccgtt cagctgtgcg ccctgaccgc
    36001 gagtggcggt gaacagttcg ttacgcatag gatcgtatac cacagcaact tcggtgcggc
    36061 ctttgatacg aacggcgata gataccgcga agtgcggcag acgtttgata aagttggtag
    36121 tgccatccag tggatcgata acccattgaa catcctgatc agtaccttca agttcaccgc
    36181 tttcttcggt gatgatggtg tgctgtgggt aagatttacg aatcgtgtcg ataatcaccg
    36241 cttcggcagc tttatctacg ttggtcacga aatcgttact gtctttctgg ctcgcttcta
    36301 cagcgtccgg ggtttcatag tttttggcaa ttaaattacc cgccttgcgc gctgcgcgca
    36361 cggcgatgtt cagcatcgga tgcatcggtc tctctcactg gatgttaaag aacgggaaaa
    36421 cggcgcagag tatagcagcg agatcggcat ctgtcttcgt tttatgataa gatgcgtgaa
    36481 taatctttag atgaagaaag acaatgctgc aaaatattcg aattgtgctg gtggagacgt
    36541 cacacaccgg caatatgggt tctgttgccc gtgccatgaa aacaatggga ttaaccaatc
    36601 tgtggctggt taatccactg gtgaaacccg actcccaggc gattgccctg gcagcagggg
    36661 ccagcgatgt gattggtaat gctcacatcg tcgatacgct cgacgaagcg ttagccggtt
    36721 gtagcctggt ggtgggcacc agcgcacgct cccgcacgct gccgtggccg atgctcgacc
    36781 cgcgcgaatg cggtctgaaa agcgtcgctg aagcggcaaa taccccggtg gcgctggtgt
    36841 ttggtcgtga gcgcgtcggc ttgaccaatg aagagttgca gaaatgccat tatcatgtcg
    36901 cgattgcggc taacccggaa tacagctcgc tgaacctggc gatggcggtt caggttattg
    36961 cctatgaagt gcgcatggcc tggctggcaa ctcaggaaaa cggcgagcag gtcgagcatg
    37021 aagagacgcc gtatccgctg gtcgatgatc tggagcgttt ttatggtcat ctggaacaaa
    37081 cgctgctggc aaccggtttt atccgtgaaa accatccggg gcaggtgatg aataaattgc
    37141 gccgtctgtt tacccgtgcg cgcccggaaa gccaggaatt gaatatcctg cgcgggattc
    37201 tggcttctat tgagcagcag aataaaggta acaaggccga ataacagtag ttgcctgatg
    37261 cgacgcttaa cgcgtcttat caggcctaca atgtacagaa ccgtaggtcg gataaggtgt
    37321 tcacaccgca tccgacaaca ggtacaaacg ccacgataaa aaaatggcac tgaaggttaa
    37381 atacccgact aaatcagtca agtaaatagt tgaccaattt actcgggaat gtcagacttg
    37441 accctgctat gcaatacccc cacttttaca ataaaaaacc ccgggcaggg gcgagtttga
    37501 ggtgaagtaa gacatgagac tgacatctaa agggcgctat gccgtgaccg caatgcttga
    37561 cgttgcgctc aactctgaag cgggcccggt accgttggct gatatttccg aacgtcaggg
    37621 aatttccctt tcttatctgg aacaactgtt ttcccgtctg cgtaaaaatg gtctggtttc
    37681 cagcgtacgt ggaccaggcg gtggttatct gttaggcaaa gatgccagca gcatcgccgt
    37741 tggcgaagtg attagcgccg ttgatgaatc tgtagatgcc acccgttgtc agggtaaagg
    37801 cggctgccag ggcggcgata aatgcctgac ccacgcgctg tggcgtgatt tgagcgaccg
    37861 tctcaccggt tttctcaaca acattacttt aggcgaactg gttaataacc aggaagtgct
    37921 ggatgtgtct ggtcgtcagc atactcacga cgcgccacgc acccgcacac aagacgcgat
    37981 cgacgttaag ttacgcgctt aataaaaaga attcagaatc aggccggagt gctaaatact
    38041 ccgtaaacac ggtcgtacat ccagccggta gcctgattcc ttgcattgag tgatgtacgg
    38101 agtttataga gcaatgaaat taccgattta tctcgactac tccgcaacca cgccggtgga
    38161 cccgcgtgtt gccgagaaaa tgatgcagtt catgacgatg gacggaacct ttggtaaccc
    38221 ggcctctcgt tctcaccgtt tcggctggca ggctgaagaa gcggtagata tcgcccgtaa
    38281 tcagattgcc gatctggtcg gcgctgatcc gcgtgaaatc gtctttacct ctggtgcaac
    38341 cgaatctgac aacctggcga tcaaaggtgc agccaacttt tatcagaaaa aaggcaagca
    38401 catcatcacc agcaaaaccg aacacaaagc ggtactggat acctgccgtc agctggagcg
    38461 cgaaggtttt gaagtcacct acctggcacc gcagcgtaac ggcattatcg acctgaaaga
    38521 acttgaagca gcgatgcgtg acgacaccat ccttgtttcc atcatgcacg taaataacga
    38581 aatcggcgtg gtgcaggata tcgcggctat cggcgaaatg tgccgtgctc gtggcattat
    38641 ctatcacgtt gatgcaaccc agagcgtggg caaactgcct atcgacctga gccagttgaa
    38701 agttgacctg atgtctttct ccggtcacaa aatctatggc ccgaaaggta tcggtgcgct
    38761 gtatgtacgt cgtaaaccgc gcgtacgcat cgaagcgcaa atgcacggcg gcggtcacga
    38821 gcgcggtatg cgttccggca ctctgcctgt tcaccagatc gtcggaatgg gcgaggccta
    38881 tcgcatcgca aaagaagaga tggcgaccga gatggaacgt ctgcgcggcc tgcgtaaccg
    38941 tctgtggaac ggcatcaaag atatcgaaga agtttacctg aacggtgacc tggaacacgg
    39001 tgcgccgaac attctcaacg tcagcttcaa ctacgttgaa ggtgagtcgc tgattatggc
    39061 gctgaaagac ctcgcagttt cttcaggttc cgcctgtacg tcagcaagcc tcgaaccgtc
    39121 ctacgtgctg cgcgcgctgg ggctgaacga cgagctggca catagctcta tccgtttctc
    39181 tttaggtcgt tttactactg aagaagagat cgactacacc atcgagttag ttcgtaaatc
    39241 catcggtcgt ctgcgtgacc tttctccgct gtgggaaatg tacaagcagg gcgtggatct
    39301 gaacagcatc gaatgggctc atcattaatc ggtatcggaa tcaggagaat ttataatggc
    39361 ttacagcgaa aaagttatcg accattacga gaatccgcgt aacgtgggtt cctttgacaa
    39421 caacgacgag aacgtcggca gcggcatggt gggtgcaccg gcctgtggcg acgtgatgaa
    39481 gttgcagatt aaagtcaacg atgaaggtat cattgaagac gcgcgtttta aaacttacgg
    39541 ctgcggttct gctatcgctt ccagctccct ggtcaccgaa tgggtgaaag ggaagtctct
    39601 cgacgaagcg caggcgatca aaaacaccga tattgctgaa gaacttgaac tgccgccggt
    39661 gaaaattcac tgttctattc tggcagaaga cgcgatcaaa gccgccattg cggactataa
    39721 aagcaaacgt gaagcaaaat aagagttgag gtttggttat gtcgattaca ctgagcgaca
    39781 gtgcagcagc gcgagtaaat acctttctgg ctaaccgcgg caaagggttt ggcctgcgtc
    39841 tgggcgtgag aacctccgga tgttcaggta tggcttatgt actggaattt gttgacgaac
    39901 cgacgccgga agacatcgtg tttgaagaca aaggcgtgaa agtcgtggtc gatggcaaaa
    39961 gcctgcaatt tctggacggt acgcagctgg acttcgtaaa agaaggcctg aatgaagggt
    40021 ttaaattcac caacccgaac gttaaagatg agtgtggttg cggcgaaagc ttccacgttt
    40081 gatgcgcata ccgataaccc caccgtggtc gcctgcgcgt ggggtttgtt ttacctgatt
    40141 cgccgttatc gcggcggatc gcagccctga gaatgttatg gattacttca ccctctttgg
    40201 cttgcctgcc cgttatcaac tcgataccca ggcactgagc ctgcgttttc aggatctaca
    40261 acgtcagtat catcctgata aattcgccag cggaagccag gcggaacaac tcgccgccgt
    40321 acagcaatct gcaaccatta accaggcctg gcaaacgctg cgtcatccgt taatgcgcgc
    40381 ggaatatttg ctttctttgc acggttttga tctcgccagc gagcagcata ctgtgcgcga
    40441 caccgcgttc ctgatggaac agctgaagct gcgcgaagaa ctggacgaga tcgaacaggc
    40501 gaaagatgaa gcgcggctgg aaagctttat caaacgtgtg aaaaagatgt ttgatacccg
    40561 ccatcagttg atggttgaac agttagacaa cgagacgtgg gacgcggcgg cggataccgt
    40621 gcgtaagctg cgttttctcg ataaactgcg aagcagtgcc gaacaactcg aagaaaaact
    40681 gctcgatttt taatttctgg aagctaaaca tggccttatt acaaattagt gaacctggtt
    40741 tgagtgctgc gccgcatcag cgtcgtctgg cggccggtat tgacctgggc acaaccaact
    40801 cgctggtggc gacagtgcgc agcggtcagg ccgaaacgtt agccgaccat gaaggccgtc
    40861 acctgctgcc atctgttgtt cactatcaac agcaagggca ttcggtgggt tatgacgcgc
    40921 gtactaacgc agcgctcgat accgccaaca ccattagttc tgttaaacgc ctgatggggc
    40981 gctcgctggc tgatatccag caacgctatc cgcatctgcc ttatcaattc caggccagcg
    41041 aaaacggcct gccgatgatt gaaacggcgg cggggctgct gaactcggtg cgcgtttctg
    41101 cggacatcct aaaagcactg gcggcgcggg caactgaagc cctggcaggc gagctggatg
    41161 gtgtagttat caccgttccg gcgtactttg acgatgccca gcgtcagggc accaaagacg
    41221 cggcgcgtct ggcgggcctt cacgtcctgc gcttacttaa cgaaccgacc gctgcggcta
    41281 tcgcctacgg gctggattcc ggtcaggaag gcgtgatcgc cgtttatgac ctcggcggcg
    41341 ggacgtttga tatttccatt ctgcgcttaa gtcgcggtgt gtttgaagtg ctggcgaccg
    41401 gcggtgattc cgcgctcggt ggtgatgatt tcgaccatct gctggcggat tacattcgcg
    41461 agcaggcggg cattcctgat cgtagcgata accgcgttca gcgtgaactg ctggatgccg
    41521 ccattgcagc caaaatcgcg ctgagcgatg cggactccgt gaccgttaac gttgcgggct
    41581 ggcagggcga aatcagccgt gaacaattca atgaactgat cgcgccactg gtaaaacgaa
    41641 ccttactggc ttgtcgtcgc gcgcttaaag acgcgggtgt agaagctgac gaagtgctgg
    41701 aagtggtgat ggtgggcggt tctactcgcg tgccgctggt gcgtgaacgg gtaggcgaat
    41761 ttttcggtcg tccaccgctg acttccatcg acccggataa agtcgtcgct attggcgcgg
    41821 cgattcaggc ggatattctg gtgggtaaca agccagacag cgaaatgctg ctgcttgatg
    41881 tgatcccact gtcgctgggc ctcgaaacga tgggcggcct ggtggagaaa gtgattccgc
    41941 gtaataccac tattccggtg gcccgcgctc aggatttcac cacctttaaa gatggtcaga
    42001 cggcgatgtc tatccatgta atgcagggtg agcgcgaact ggtgcaggac tgccgctcac
    42061 tggcgcgttt tgcgctgcgt ggtattccgg cgctaccggc tggcggcgcg catattcgcg
    42121 tgacgttcca ggtcgatgcc gacggtcttt tgagcgtgac ggcgatggag aaatccaccg
    42181 gcgttgaggc gtctattcag gtcaaacctt cttacggtct gactgacagc gaaatcgctt
    42241 cgatgatcaa agactcaatg agctatgccg agcaggacgt aaaagcccga atgctggcag
    42301 aacaaaaagt agaagcggcg cgtgtgctgg aaagtctgca cggcgcgctg gctgctgatg
    42361 ccgcgctgtt aagcgccgca gaacgtcagg tcattgacga tgctgccgct cacctgagtg
    42421 aagtggcgca gggcgatgat gttgacgcca tcgaacaagc gattaaaaac gtagacaaac
    42481 aaacccagga tttcgccgct cgccgcatgg accagtcggt tcgtcgtgcg ctgaaaggcc
    42541 attccgtgga cgaggtttaa tatgccaaag attgttattt tgcctcatca ggacctctgc
    42601 cctgatggcg ctgttctgga agctaatagc ggtgaaacca ttctcgacgc agctctgcgt
    42661 aacggtatcg agattgaaca cgcctgtgaa aaatcctgtg cttgcaccac ctgccactgc
    42721 atcgttcgtg aaggttttga ctcgctgccg gaaagctcag agcaggaaga cgacatgctg
    42781 gacaaagcct ggggactgga gccggaaagc cgtttaagct gccaggcgcg cgtcaccgac
    42841 gaagatttag tggtcgaaat cccgcgttac actatcaacc atgcgcgtga gcattaacag
    42901 aggttagtat gggacttaaa tggaccgata gccgcgaaat tggcgaagca ctgtacgatg
    42961 cgtatcccga tcttgatccg aaaacggttc gattcaccga tatgcatcag tggatttgcg
    43021 atctggaaga tttcgacgac gacccgcagg catccaacga gaaaatcctc gaagcgattt
    43081 tgttagtctg gctggacgag gccgaataat ttccgcgtca tgcttcacgc cgcagatgcg
    43141 ttggctgcgc tcgttcaccc cagtcacata gttatctatg ctcctggggc ttcactcact
    43201 tgccgccttc ctgcaacgcg aatcatttag cggaaaaatc ctggggctgc caactggcgg
    43261 cccttttaca aagaaggata actaaagtga cagaagcgat gaagattacc ctctctaccc
    43321 aacctgccga cgcgcgctgg ggagaaaaag caacttacag cattaataat gacggcatta
    43381 ccctgcattt gaacggggca gacgatctgg ggctgatcca gcgtgcggcg cgcaagattg
    43441 acggtctgag catcaagcat gttcagttaa gcggtgaagg ctgggatgcg gatcgctgct
    43501 gggcattctg gcaaggttac aaagccccga aaggcacgcg taaagtggag tggccggatc
    43561 tggacgatgc ccagcgccag gaactggata accgcctgat gatcatcgac tgggtgcgtg
    43621 acaccatcaa cgcaccggca gaagaattgg gaccatcgca actggcacag cgtgctgttg
    43681 atctgatcag caacgtcgcg ggcgatcgtg tgacttatcg gatcaccaaa ggcgaagatc
    43741 tgcgtgagca aggttatatg gggctgcaca ccgtcggacg cggttcagaa cgttctccgg
    43801 tattgctggc gctggattac aacccaactg gcgataaaga agcgccagtg tacgcgtgcc
    43861 tggtaggtaa aggtatcact tttgactccg gcggctacag catcaaacag actgcgttta
    43921 tggactcgat gaagtcggac atgggcggcg cggcaacggt taccggggcg ctggcatttg
    43981 ccattacgcg cggactgaac aagcgcgtga agctgttcct ctgctgtgcg gataacctga
    44041 ttagcggcaa tgcgttcaag ctgggcgata tcatcaccta tcgcaacggt aaaaaagttg
    44101 aagtgatgaa cactgatgcc gaagggcgtc tggtgcttgc cgatggtctg attgatgcca
    44161 gtgcgcagaa accggaaatg atcattgatg cggcgaccct caccggggcg gcgaaaactg
    44221 cgctgggtaa tgattatcac gcgctgttca gttttgacga tgcgcttgcc ggtcgtttgc
    44281 tggcgagtgc ctcacaagag aacgaaccat tctggcgtct gccgctggcg gaattccacc
    44341 gcagccagct gccgtctaac tttgccgaac tgaacaatac cggaagcgcg gcgtatccgg
    44401 caggcgcgag cacggcggcg ggcttcctgt cgcactttgt tgagaactat cagcaaggct
    44461 ggctgcatat cgactgctcg gcgacttacc gtaaagcgcc ggttgaacag tggtctgcgg
    44521 gcgctacggg acttggtgtg cgcacgattg ctaatctgtt aacggcgtaa actaatatgc
    44581 cggatgcggc gtaaacgcct tatccggcct acaaattctg caaattcaac aaattgcgaa
    44641 tcccttgtag gcctgataag cgtagcgcat caggcatttg cgccccgaac gttgaatgaa
    44701 ttgtggttct tatgtccgaa acaaaaaacg aacttgaaga cctgctggaa aaagcagcaa
    44761 ccgaaccggc gcaccgcccg gcctttttcc gtactctact ggaatccacc gtctgggtgc
    44821 ctggtacggc ggcgcagggc gaggctgtgg ttgaagatag tgcgcttgat ttacagcact
    44881 gggaaaaaga agacggcacc agcgtcattc cttttttcac ctcgttagaa gcgcttcaac
    44941 aggcggttga agacgaacag gcatttgtcg taatgcccgt tcgcacattg tttgagatga
    45001 cccttggcga aacgctcttc cttaatgcca aactgccgac cggtaaagaa tttatgccgc
    45061 gtgaaatcag tttgttgatt ggtgaagagg gaaatccgct gagcagccag gaaatcctgg
    45121 aaggcggtga atcgctgata ttatcggaag tcgcagagcc gccagcacaa atgattgatt
    45181 cactcaccac cttatttaaa accattaagc cggtgaagcg tgcttttatt tgttcaatta
    45241 aagagaacga agaggcacag cctaatttac ttattggcat tgaagccgat ggtgatatcg
    45301 aagaaattat tcaggcgacg ggaagtgtag cgaccgatac attacctggc gatgaaccaa
    45361 tcgatatttg tcaggtgaaa aaaggggaaa aaggaattag ccactttatt accgaacata
    45421 ttgtgccatt ctatgaacgt cgctggggtg gttttttgcg tgactttaaa caaaaccgga
    45481 taatctaaaa tatgccgccc taaagggcgg cattcgggct tacttgctgc tctgaaggtt
    45541 gttaaccacc agtaaattgt cgccggagag actccggtta ttatttactt gggaaaacag
    45601 cgcaccagaa acaataagta cgatacagaa gcaccgtatc ttcattgtgc ggtccatgcg
    45661 aagaccgcat cacgacagca ccactggcga tattgccgcg atagcaagcg ctgagacgcg
    45721 cttgctctaa cggtcgcgga acatcctttc cttaagccag cgcccgcttg cgagacggcg
    45781 gaaagggccg cagtggataa tacactaaca gaacaataat aactctcttt tgcttgacaa
    45841 aaagagagtt actggcgagt attgtctccg ctgagacgcg cttgcattac tacggtaata
    45901 caacctgctg gaaaggccgc gaaccagacc agcaataaaa aaccgccaaa ttcggcggtt
    45961 ttttattgcc tgtaaaatac ttatttcact ggctcaaccg gtaaatctgc ccgcgcgccc
    46021 cattcactcc atgcgccgtc gtacagtttc acgtttggca catccagcgt cgcgagtgct
    46081 aacaaaacca cggctgccgt tacaccagag ccgcagctga cgataattgg tttgtcgtag
    46141 ctgacgccgc gaccaaaaaa tatcgcatcc agttcatcgg tcgtttttag ttcgccttcg
    46201 cgcaccagtt ccgtccacgg aacattcagt gctccgggaa tatgtccgcg acgtaaacct
    46261 gggcgaggtt catcaacttc tgcgttaaaa cgtgcagccg ggcgggcatc aataatttgc
    46321 gccgtatttt catggcttgc caataataca tcggttactt tcaccacggc ttcaggatta
    46381 aacgcggcgt taaactctcc ttccggcagc tctactgcac cttcttctaa cagcagatca
    46441 tcgcgctgcc agcctgcaag tccacccccc agaatcgaca ctttctctac accaaaggtg
    46501 cgcagcatcc accatgctcg tggggctgag aaaagattac cttcgtcata gacaatcagg
    46561 tgcttatcct ggttaacgcc taattcacgc atcgccacgg cgaacgtttc cgggcgcggc
    46621 agcatgtgcg gaagcgggga agtgtgatca gaaagcgctt cgatatcaaa aaacactgcg
    46681 ccgggaatat gtccattcag atactcctga gcaacgttac gatcttcctg tccaggcgac
    46741 gccatgcggg catcgataat ctgaatttcc gggtcatcaa tatgttcggc gagccagtcg
    46801 gctcctacaa accatgtcgt ggacataggc atctccattt ttgccggggt taggctaaat
    46861 tgtcggatgt ttagtgaaaa gcgacaagcg aaagaggctg atttggctgt ctgagccaca
    46921 attctcacta aaattcggat tgtatgggcg tgatactgaa ttacataacg taaattctca
    46981 cgcataataa ttattcgacg ttgctaacgg gccattgtgg cagggatgaa aaatgaaaaa
    47041 gttacgcgta gccgcctgca tgctaatgct ggcgctggca gggtgcgaca acaacgataa
    47101 cgcgccaaca gcggtgaaaa aagatgcgcc ttctgaagtt actaaagcgg cctcttcaga
    47161 aaacgcgagt tcagcaaaac tctccgtgcc ggagagacaa aaactggccc aacagagtgc
    47221 cggtaaggtg ctgacattgc tggatctctc tgaagtccaa cttgatggtg cagccacgct
    47281 ggtgctgacg ttctccatcc ctctcgaccc ggatcaggat ttctcacgcg ttattcatgt
    47341 cgtcgataaa aaaagcggca aagtggatgg tgcctgggag ctgtcagata atcttaaaga
    47401 gctacgttta cgccacctcg aaccgaaacg tgatttgatc gttactattg gcaaggaggt
    47461 caaagcactc aacaacgcaa ccttcagtaa agattacgaa aaaactataa ctacccgcga
    47521 catccaaccc agcgtcggtt ttgccagccg tggttcgctg ctgcctggca aagtcgttga
    47581 agggctgccg gtaatggcgc tcaacgttaa taatgtcgat gttaacttct tccgcgttaa
    47641 gccagaatct ctgccagcat tcattagcca atgggaatac cgcaattcgc tggcgaactg
    47701 gcagtcagac aaactgctgc agatggcgga tctggtctac accggacggt ttgatctcaa
    47761 tcctgcgcgt aacacccgtg aaaaattatt gctgccgctg ggcgatatca aaccgcttca
    47821 gcaggcgggc gtgtatctgg ctgtgatgaa tcaggctgga cgttacgatt acagtaatcc
    47881 cgcgacgctg tttacgttaa gtgatatcgg cgtttcagct caccgttatc acaatcgtct
    47941 ggatatcttt acccaaagtc tggaaaacgg cgcggcccag caaggaattg aagtctcttt
    48001 attaaatgag aaagggcaga ctctgactca ggcaaccagt gacgctcagg ggcatgtgca
    48061 gctggaaaat gataaaaatg cggcattact gctggcacgt aaagacggtc agacaacgct
    48121 actcgattta aaacttccgg cgctggactt agcagaattt aacattgctg gcgcgccagg
    48181 ctatagcaaa cagtttttca tgtttggccc acgcgatctt tatcgcccag gtgaaacggt
    48241 aatcctcaat ggtttactgc gcgatgcaga cggtaaagca ttgcccgatc aacccatcaa
    48301 gttagacgtg attaaacctg acgggcaggt actcaggagc gtcgttagtc agccggagaa
    48361 tggcctctac cactttacct ggccactcga tagcaatgcg gcaaccggta tgtggcatat
    48421 tcgcgccaac acgggcgata accagtaccg gatgtgggat ttccacgtcg aagattttat
    48481 gccagagcgt atggcgctga atctgaccgg tgagaaaacc ccgcttacgc cgaatgatga
    48541 agtgaaattc tccgtggtgg gatactacct gtacggtgcg cctgctaatg gtaatacttt
    48601 gcaagggcaa cttttcctgc gcccactgcg tgaggctgtg tcagccttac ctggttttga
    48661 attcggcgat atagctgccg aaaacctttc ccgcacgctg gatgaagttc agttgacgct
    48721 ggatgataaa gggcgcggcg aagtttctac agaaagccag tggaaggaaa cgcattcccc
    48781 attgcaggtt attttccagg gcagtttgct ggaatcgggc ggtcgcccgg tgacgcgccg
    48841 cgctgagcag gctatctggc ctgccgatgc attgccgggg ctccgtccgc agttcgcctt
    48901 gaaatcggtt tacgattatc gcactgacag cacggtgaaa cagcccattg ttgatgaagg
    48961 cagtaacgcc gcttttgaca tcgtttatag cgatgcacaa ggcgtgaaaa aagccgtgtc
    49021 gggcttgcag gtgcgcctga ttcgcgaacg ccgcgattac tactggaact ggtcagaaga
    49081 tgaaggctgg cagtcacagt ttgatcaaaa agatctgatc gaaaatgaac aaactctgga
    49141 tctgaaagcg gacgaaaccg gtaaggtcag ttttccggta gagtggggcg cttatcgtct
    49201 ggaagtcaaa gcgccgaatg aagcggtcag tagtgttcgt ttctgggctg gctatagctg
    49261 gcaggacaac agcgacggta gcggcgcagt gcgacccgac cgtgtcacgc tgaaactgga
    49321 taaagccagt tatcgccctg gcgacaccat taagttgcat atcgccgcgc caacggcggg
    49381 taaaggttat gcgatggtcg agtccagtga agggccgctg tggtggcaag agattgatgt
    49441 tccggctcaa gggctggatc tgacgattcc ggtcgataaa acctggaatc gtcatgatct
    49501 gtatttaagt acgctggtgg tacgtcctgg cgataaatct cgctccgcga cgccaaaacg
    49561 cgcggttggt gtgttgcatc tgccgcttgg cgatgaaaac cgtcgcctcg atctggcgct
    49621 ggaaacacca gcaaaaatgc gtcccaatca accattaacc gtgaaaatta aagccagcac
    49681 taaaaatggc gagaagccta aacaggtgaa tgtgctggtg tctaccgttg atagtggtgt
    49741 gctgaatatt actgactacg tcacgccaga tccgtggcag gcgttctttg gtcagaaacg
    49801 ctatggcgca gacatttacg atatttacgg tcaggttatt gaaggtcagg ggcgtctggc
    49861 agctctgcgt ttcggtggcg atggtgatga gctgaaacgt ggtggtaaac cgccggtcaa
    49921 tcacgtcaat attgtcgcgc agcaggcgct gccggtaacg ctcaacgaac agggcgaagg
    49981 ctcggttaca ctgccgattg gcgattttaa cggtgaattg cgcgtcatgg cgcaagcctg
    50041 gacggcagat gatttcggta gcaacgaaag caaagtgata gttgccgcac cggtgattgc
    50101 tgaactgaac atgccgcgct ttatggcgag tggcgatacc tcgcgtctga cgctggatat
    50161 cactaatatt accgataaac cgcaaaaact gaacgttgcc ctgaccgcca gtggtttgct
    50221 tgaactggtc agcgattcac ccgcagccgt tgaattagcg ccaggtgtgc gtactacgct
    50281 gtttatcccg gtgcgagcat tgccgggtta tggcgatgga gaaattcagg ccaccattag
    50341 cgggttagcg ttaccgggtg aaaccgttgc cgatcagcat aagcagtgga aaatcggcgt
    50401 ccgtccggcg ttcccggcac aaacggttaa ttacggtacg gcgttacagc ctggtgagac
    50461 atgggcgatt ccggcggatg gattgcaaaa cttctcgcct gttacgctgg aagggcaatt
    50521 gttgttgagc ggcaaaccac cgctgaacat tgcacgttat atcaaagagt taaaagcgta
    50581 tccgtacggc tgtcttgagc aaaccgccag cggcctgttc ccgtcacttt ataccaacgc
    50641 agcccaactg caggcgttgg gcatcaaagg cgacagtgat gagaaacgcc gtgcatcggt
    50701 cgatatcggc atttcccgtt tgctgcaaat gcaacgtgat aacggcggct ttgcgctgtg
    50761 ggataaaaac ggtgacgaag agtactggct gacggcttac gtgatggatt tcctggtccg
    50821 cgcaggcgaa cagggttaca gcgtgccgac agacgccatt aaccggggta atgagcgtct
    50881 gctgcgctat ttacaagatc cgggcatgat gtcgatcccg tacgcggata atctcaaagc
    50941 cagtaaattc gccgttcagt cttacgctgc gctggtgctg gcccgtcagc aaaaagcacc
    51001 actgggtgcg ctgcgtgaaa tctgggagca tcgtgcagat gccgcttctg gtttaccgct
    51061 gctgcaactt ggcattgcgc tgaaaaccat gggtgatgcg acgcgtggtg aagaagcgat
    51121 tgcgctggcg ctgaaaacgc cgagaaatag tgatgagcgg atatggctgg gtgattacgg
    51181 tagtccactg cgcgacaacg cgttaatgct ctccttgctg gaagaaaata aactgctacc
    51241 cgatgagcag tacactttgc tgaacacact ttcgcagcag gcgtttggtg aacgctggct
    51301 atcgacgcag gaaagtaacg cgttgttcct ggctgcccgt acgattcagg atttaccggg
    51361 taaatggcag gcgcaaacct ctttctcagc tgagccgctg acaggcgaga aaacgctaaa
    51421 cagcaatctg aatagcgatc aacttgccac cttgcaggtg agaaacagtg gcgatcagcc
    51481 gttatggttg cgtatggatg ccagcggtta tccgcaatcc gcacctttac cggcgaacaa
    51541 tgtgctgcaa atcgagcgtc atattcttgg tactgatggt aagagcaaat cgctggactc
    51601 gttacgtagc ggcgatctgg tgctggtctg gttgcaggta aaagccagta acagcgtgcc
    51661 ggatgcgtta gtcgtggatc tgctgcctgc gggtctggaa ctggaaaatc agaatctggc
    51721 gaacggtagc gccagcctgg agcaaagtgg tggcgaagtg caaaacttac tgaaccagat
    51781 gcagcaggcg agcattaagc acattgagtt ccgtgacgat cgctttgtgg cggcggttgc
    51841 cgttgatgaa taccaaccgg taacgctggt gtatctggcg cgggcggtga cgccgggaac
    51901 gtatcaggta ccgcaaccga tggtggaatc aatgtatgtt ccccaatggc gggcgaccgg
    51961 cgcggctgaa gatctgctga ttgtccgacc gtaaatgctt cgcttgttaa ccaaacgcgg
    52021 ctgctggata acgctggcag ctgcgccatt tctccttttt cttgcagcgt ggggagcaga
    52081 taaactctgg cctctaccgc tgcatgaagt caatcccgca cgagtggtcg tggcgcagga
    52141 tggtacgccg ctctggcgct tcgccgatgc tgatggcatc tggcgttatc cggtaacaat
    52201 cgaagatgtg tctccacgtt accttgaagc gctgatcaat tatgaagatc gctggttctg
    52261 gaagcatccg ggggtgaatc cattctcggt ggcgcgcgca gcatggcaag atctcacttc
    52321 gggacgggtt atttctggtg gcagcacgct cactatgcag gttgctcgtc tgcttgatcc
    52381 tcaccccaaa acatttggtg gcaaaattct ccagctctgg cgcgcgttgc aactggagtg
    52441 gcatttgtct aagcgtgaaa ttctgatctt gtatcttaac cgcgctccgt ttggcggtac
    52501 gttgcagggg atcggtgcgg caagttgggc ttatctcgga aaatcgcctg cgaatttaag
    52561 ctattccgag gcggcaatgc tggcggtgtt accgcaagcg cccagccgac tgcgcccgga
    52621 tcgttggccg gagcgtgccg aagccgcgcg taataaagtg ctcgaacgga tggccgtgca
    52681 aggtgtgtgg tcccgtgagc aggtaaaaga gtcaatggaa gaacccatct ggctggcacc
    52741 ccgacaaatg ccgcaactgg caccgctgtt ttcgcgcatg atgctcggta aaagcaaaag
    52801 cgacaaaatc gttactacgc tggatgccgg tcttcaacga cgtctggaag aactggcgca
    52861 aaactggaaa gggcggttgc caccgcgcag ctcactggcg atgatcgtgg ttgatcatac
    52921 cgatatgcgt gttcgcggct gggtgggatc ggttgatctc aacgatgatt cacgctttgg
    52981 tcatgttgat atggtcaatg cgatccgatc gccaggatcg gtgctcaaac cgtttgttta
    53041 tggtctggcg ctggatgaag gcttgatcca cccggcatca ctgctgcaag acgtcccccg
    53101 gcgcaccggt gattatcgac caggtaactt tgatagcggt tttcatggcc cgatcagcat
    53161 gagcgaggcg ctggtgcgct cgctgaactt acctgctgtg caggtgctgg aagcctatgg
    53221 accgaaacgg tttgcggcaa agttacgcaa tgttggattg ccgttatatt tgcccaacgg
    53281 tgctgcgccg aatctttcac tcattctcgg cggcgctggt gcaaaactgg aagatatggc
    53341 ggcagcgtat accgcgtttg ctcgccacgg caaggcaggc aaattgcgct tacagcctga
    53401 cgatccgctg cttgaacggc ctttaatgtc gtcgggggcg gcgtggatca ttcgccggat
    53461 tatggctgat gaagcgcaac ccttgccgga tagtgccttg ccgcgcgtcg ccccactggc
    53521 atggaaaacg ggcaccagct atggctatcg tgacgcctgg gcgattgggg ttaacgctcg
    53581 ctatgtcatt gggatctgga ctggcagacc ggacggcacg cccgttgttg gtcagtttgg
    53641 ctttgccagt gccgtaccat tgttgaatca ggtcaataat atcttactgt cgcgcagtgc
    53701 gaatctgccg gaagacccgc gcccgaactc agtcactcgt ggcgttatct gctggccggg
    53761 cggacagtct ttgccggaag gtgacggtaa ctgtcgccgc cgcctggcaa cctggctgct
    53821 ggacgggagt cagccgccaa ctctattact gccggagcag gaaggcatta atggcattcg
    53881 tttccccatc tggctggatg aaaatggcaa acgtgttgcc gctgattgcc cgcaagcgcg
    53941 acaagaaatg ataaatgtct ggccgctacc gctggaaccc tggctgcccg catcagaacg
    54001 tcgcgctgtg cgcttaccac cggcctcgac aagttgcccg ccatacggtc acgatgccca
    54061 gctcccgctg cagctgacag gcgttcgcga tggcgcgatt attaaacgtt taccgggcgc
    54121 agcggaagcg actttgccgt tgcaatcaag tggaggggca ggtgaacgct ggtggtttct
    54181 gaatggcgaa ccgttaactg aacgcgggcg caacgtgacc ctgcatttga cggataaagg
    54241 tgattatcaa ttgctggtga tggatgacgt ggggcaaatc gcgacagtga aatttgtcat
    54301 gcaatagtct gttttgtccc tgattgttgc taaaactcat tttattttaa aaaaatgtta
    54361 ccttcatcaa tagtcaacgg ctctgttgct cattataatc cgcgccatct cgtacgctgg
    54421 tacagacaac aacagaacaa tttacagagg taaaaatggc tattgaacgt actttttcca
    54481 tcatcaaacc gaacgcggta gcaaaaaacg tcattggtaa tatctttgcg cgctttgaag
    54541 ctgcagggtt caaaattgtt ggcaccaaaa tgctgcacct gaccgttgaa caggcacgtg
    54601 gcttttatgc tgaacacgat ggaaaaccgt tctttgatgg tctggttgaa ttcatgacct
    54661 ctggcccgat cgtggtttcc gtgctggaag gtgaaaacgc cgttcagcgt caccgcgatc
    54721 tgctgggcgc gaccaatccg gcaaacgcac tggctggtac tctgcgcgct gattacgctg
    54781 acagcctgac cgaaaacggt acccacggtt ctgattccgt cgaatctgcc gctcgcgaaa
    54841 tcgcttattt ctttggcgaa ggcgaagtgt gcccgcgcac ccgttaataa tttcgtaaat
    54901 gccgcgtgca aacgtggcat ccgtgcgcca gaatttgtac aatgcagcgc ccccggacga
    54961 gcagccgctc accggggcgt ttcttttttc aaccctccag gggccataac gtgtaataac
    55021 gaggccggaa tagcatatgt ctgaacaatt agtcacacct gaaaacgtca ccacgaaaga
    55081 tggaaaaatc aacctgctgg atctcaaccg tcagcagatg cgggagtttt ttaaagattt
    55141 aggtgaaaaa cccttccgcg ccgatcaggt gatgaagtgg atgtatcact attgctgcga
    55201 caactttgat gagatgaccg acatcaacaa agtgttgcgc ggcaaactga aagaggtggc
    55261 ggagatccgc gcaccggaag tggttgaaga acagcgttca tccgacggca ccattaaatg
    55321 ggcgatcgcc gttggcgatc agcgcgtcga aacggtgtat atcccggaag acgaccgtgc
    55381 cacgctctgc gtctcttcgc aggtggggtg tgcgctggag tgtaaattct gttccaccgc
    55441 ccagcagggc tttaaccgca acctgcgggt gtcggaaatt atcggccagg tgtggcgtgc
    55501 ggcgaaaatc gtcggcgcag caaaagtcac cggtcagcgc ccaatcacta acgtagtgat
    55561 gatgggcatg ggcgagccgc tgctcaacct gaacaacgtc gttccggcga tggaaatcat
    55621 gcttgatgat ttcggttttg gcctgtctaa acgtcgcgtc acgctttcta cttccggtgt
    55681 agttccggcg ctggataaac tgggcgatat gatcgacgtt gcactggcaa tttccctgca
    55741 cgcgccgaac gacgaaattc gtgacgaaat tgtgccgatc aacaaaaagt acaatatcga
    55801 aacgttcctt gctgcggtgc gccgttatct ggagaaatcc aacgccaatc agggccgagt
    55861 cactattgaa tacgtgatgc ttgaccacgt taacgacggc actgaacacg cgcaccaact
    55921 ggcggagctg ctgaaagata cgccgtgtaa gatcaacctg atcccgtgga acccgttccc
    55981 gggcgcgccg tatggacgca gttcgaacag ccgtatcgac cgtttctcaa aagtgctgat
    56041 gagctacggt ttcaccacta ttgtgcgtaa aactcgtggt gatgatatcg atgctgcctg
    56101 tggtcagttg gcgggcgatg ttatcgaccg tacgaaacgt accctgcgta aacggatgca
    56161 gggtgaagcc atcgacatta aagcggtctg atagccatta cgccacggtt actttatgta
    56221 ccgtggcgta atcttttatg aatgaagtat taagttgcaa ttgtctggtc aatattaacg
    56281 gtgcgttttt gctgacttta aggcagtatg taacgatgca acagtaagtt agccttagtg
    56341 aatgtgggct ttgtcacgag cacacagacg gtcttatact gtatgataac ggttaactta
    56401 acggatgttt cgcggtgtgg gtgagcatga tggttcaccg gcatctcaat tctcatttaa
    56461 acgtacctgc agcgaatgaa tactgaagcc acgcacgacc aaaatgaagc acttactacc
    56521 ggcgctcgcc tgcgtaatgc tcgcgaacaa ctaggactta gtcagcaggc cgttgccgag
    56581 cgactttgcc tgaaggtttc cacggtacgc gacattgaag aagataaggc acccgccgat
    56641 cttgcttcaa cattcctgcg cggatatatc cgctcttatg cgcgtctggt acatattcca
    56701 gaagaagaac tgctgccagg gctggaaaag caggctccac tacgggctgc gaaagttgcg
    56761 ccgatgcaga gtttttccct cggtaaacgc cgcaaaaaac gtgacggctg gctgatgacc
    56821 ttcacctggc tggtgttgtt tgtggttatc ggcctgagcg gtgcctggtg gtggcaagac
    56881 cacaaagctc agcaggaaga gatcaccact atggccgatc aatcttcggc ggaactgagc
    56941 agtaatagcg agcaggggca gagtgttccg ttaaatacgt cgacaactac agacccggct
    57001 acaacgtcca cgccgccagc gtctgtggat actaccgcaa ccaacacgca aacacctgcc
    57061 gtaactgcgc cagcaccagc tgttgatccg caacagaatg cggttgtttc gccttcgcag
    57121 gcaaatgttg ataccgccgc gaccccggca ccaacggcag caacaacgcc agatggtgct
    57181 gcgccgttgc caaccgatca ggctggcgtg accacgccgg tggctgatcc gaatgcgctg
    57241 gtgatgaact ttactgccga ttgctggctg gaggtcactg atgctaccgg taaaaaattg
    57301 tttagcggta tgcagcgtaa agacggtaat ttgaacttaa ccggccaggc accgtacaaa
    57361 ctgaaaattg gtgcgccagc cgcagtacag atccagtatc aagggaaacc tgtcgatctg
    57421 agtcgtttta tcagaactaa ccaggttgcg cgtctgaccc tcaatgccga acaatcaccg
    57481 gcgcagtaac agacgggtaa cgcgggagat ttttcatgca taaccaggct ccaattcaac
    57541 gtagaaaatc aacacgtatt tacgttggga atgtgccgat tggcgatggt gctcccatcg
    57601 ccgtacagtc catgaccaat acgcgtacga cagatgtcga agcaacggtc aatcaaatca
    57661 aggcgctgga acgcgttggc gctgatatcg tccgtgtctc cgtaccgacg atggacgcgg
    57721 cagaagcgtt caaactcatc aaacagcggg ttaacgtgcc gctggtggct gacatccact
    57781 tcgactatcg cattgcgctg aaagtagcgg aatacggcgt cgattgtctg cgtattaacc
    57841 ctggcaatat cggtaatgaa gagcgtattc gcatggtggt tgactgtgcg cgcgataaaa
    57901 acattccgat ccgtattggc gttaacgccg gatcgctgga aaaagatctg caagaaaagt
    57961 atggcgaacc gacgccgcag gcgttgctgg aatccgccat gcgtcatgtt gatcatctcg
    58021 atcgcctgaa cttcgatcag ttcaaagtca gcgtgaaagc gtctgacgtc ttcctcgctg
    58081 ttgagtctta tcgtttgctg gcaaaacaga tcgatcagcc gctgcatctg gggatcaccg
    58141 aagccggtgg tgcacgcagc ggggcagtaa aatccgccat tggtttaggt ctgctgctgt
    58201 ctgaaggcat cggcgacacg ctgcgcgtat cgctggcggc cgatccggtc gaagagatca
    58261 aagtcggttt cgatattttg aaatcgctgc gtatccgttc gcgagggatc aacttcatcg
    58321 cctgcccgac ctgttcgcgt caggaatttg atgttatcgg tacggttaac gcgctggagc
    58381 aacgcctgga agatatcatc actccgatgg acgtttcgat tatcggctgc gtggtgaatg
    58441 gcccaggtga ggcgctggtt tctacactcg gcgtcaccgg cggcaacaag aaaagcggcc
    58501 tctatgaaga tggcgtgcgc aaagaccgtc tggacaacaa cgatatgatc gaccagctgg
    58561 aagcacgcat tcgtgcgaaa gccagtcagc tggatgaagc gcgtcgaatt gacgttcagc
    58621 aggttgaaaa ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc
    58681 atggctcccg aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat
    58741 aaacgtggca aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac
    58801 ggccatctgg cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag
    58861 tgaaatccgc ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt
    58921 caccgacgtg gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac
    58981 tctgcgccct gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta
    59041 caatcaggaa cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa
    59101 agggcgttat cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga
    59161 tatcgacgct gaactgatta tgctcaccgc ccgctggtgg cgcgcgctgg gtatttccga
    59221 acacgtaact cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga
    59281 tgcgctggtg gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg
    59341 catgtacact aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct
    59401 caacgacgct ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg
    59461 tctgtgcaaa ctgcttgaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg
    59521 tggtctggat tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca
    59581 gggcaccgtg tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc
    59641 aacaccggct gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt
    59701 taatccggaa tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga
    59761 tacacaatct gcggctatgg cattagctga gcgtctgcgt gatgaattac agggcgtgaa
    59821 attgatgacc aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg
    59881 gggtgcccgc gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt
    59941 gaaggatttg cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt
    60001 gcgcacgtta ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac
    60061 gaccaggtag aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg
    60121 gtgattttgg gcgttggcgc actgattggc tggcgctact ggaacagcca tcaggttgat
    60181 tctgcacgct ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa
    60241 ccggatagca tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg
    60301 ctggcttctt tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc
    60361 gcccagttac aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat
    60421 ctgcgtcttg ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt
    60481 gataccatca aaggtgaagg gtgggctgcc attgttgccg acctgcgtgg tgaagcattg
    60541 ctgagcaaag gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat
    60601 gttactccgg cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag
    60661 ggaccccgat gcaattgcgt aaattactgc tgccaggact gctttccgtt acccttttaa
    60721 gcggctgttc gctgtttaac agcgaagaag atgtggtaaa gatgtcccca ttgccaaccg
    60781 ttgaaaacca gtttacgccg accacggcgt ggagtacttc cgttggtagc ggcattggca
    60841 acttctattc caatcttcat ccggcactgg cggacaacgt tgtctatgca gcggaccgcg
    60901 ctggtttggt aaaagcgctg aatgcggatg atggcaaaga aatctggtct gtcagcctgg
    60961 ccgagaaaga tggctggttc tctaaagagc ctgcattact ttctggcggt gtgaccgtgt
    61021 ctggtgggca tgtctacatt ggcagcgaaa aggcgcaggt ttacgcgctg aataccagcg
    61081 atggtactgt ggcatggcaa actaaagtcg cgggtgaagc actttcgcgc ccggtggtca
    61141 gcgacggtct ggtgttaatc cacaccagta acggtcagtt acaagcgctg aacgaagctg
    61201 acggcgctgt caaatggaca gttaacctcg atatgccttc gctctctttg cgtggcgagt
    61261 ctgcgccggc aacggctttt ggtgcggccg tcgtgggggg cgataatggt cgcgtcagcg
    61321 cagtgctgat ggaacagggc cagatgattt ggcagcagcg tatttcccag gcgaccggtt
    61381 ctaccgaaat tgaccgtctg agcgatgttg acacgactcc cgtcgttgtt aacggcgttg
    61441 ttttcgcgct ggcctataat ggtaacctga cggcgcttga tctgcgcagt ggtcagatta
    61501 tgtggaaacg cgaactgggt tcggtgaatg atttcatcgt cgacggcaat cgcatctatc
    61561 tggtcgatca aaatgacagg gtgatggcgt tgaccattga tggcggcgtt acgctgtggg
    61621 cacaaagcga tctgctgcat cgcctgctga cttctccggt gctgtataat ggcaacctgg
    61681 tggtcggtga cagtgaaggt tatctgcact ggattaacgt cgaagatggt cgtttcgttg
    61741 cccagcaaaa agttgatagt tccggtttcc agactgaacc ggttgccgct gacggcaaac
    61801 tgctgatcca ggcaaaagac ggaaccgtgt actctattac acgttaatcg tctctgtcgt
    61861 tcactttgaa aacggctcct ggacaggggc cgttttcctg tttttaacaa cgacgcgaat
    61921 atagcgtgcg ttgtctgatg atttataaaa atgaggcttt aaacatggta cctgtggtcg
    61981 cgcttgtcgg gcgccctaac gtaggaaaat ccacgttatt taaccgtcta actcgcaccc
    62041 gagatgcgct ggttgcggat ttcccgggtc tgactcgtga ccgtaagtac ggtcgtgcgg
    62101 aaattgaagg ccgtgagttt atctgtattg ataccggcgg gattgatggc acagaagacg
    62161 gtgtagaaac ccgcatggcg gaacagtcgc tgctggcgat tgaagaagcg gacgtcgtac
    62221 tgtttatggt ggatgcgcgc gcgggcctga tgccggcaga tgaagcgatt gccaaacatc
    62281 tgcgctcccg tgaaaaaccg accttcctgg tggcaaacaa aactgacggt ctggatcccg
    62341 atcaggcagt ggttgatttc tactcgcttg gcttaggtga aatctacccg atcgccgcgt
    62401 ctcacggtcg tggcgtatta agtctgctgg agcatgtgct gctgccgtgg atggaagatc
    62461 tcgcaccgca agaggaagtc gacgaagacg ctgaatactg ggcgcaattt gaagcggaag
    62521 agaacggcga agaagaagag gaagacgact tcgacccgca aagtctgccg atcaaactgg
    62581 cgattgtggg tcgtccgaac gtaggtaagt ctacactcac taaccgtatt cttggtgaag
    62641 agcgcgttgt tgtttatgac atgcctggta cgacgcgtga cagcatctat atcccaatgg
    62701 aacgcgatgg acgtgagtat gtgctcattg acaccgctgg cgtacgtaaa cgcggcaaaa
    62761 tcaccgatgc tgtagagaaa ttctccgtaa tcaaaacgtt gcaggccatt gaagacgcca
    62821 acgtggtgat gttagtgatt gatgcgcgcg aaggtatttc cgatcaggat ctctcgctgc
    62881 tgggctttat tctcaatagt gggcgctcac ttgtcattgt ggtgaataag tgggatggcc
    62941 tgagccagga agtgaaagag caggtgaaag aaacgctgga cttccgtctg ggctttatcg
    63001 attttgctcg tgtgcacttt atctctgcct tgcacggcag tggtgttggt aacttgtttg
    63061 aatcagtacg tgaagcgtat gacagctcca cccgtcgtgt ggggacctct atgctgacgc
    63121 gcatcatgac gatggctgtt gaagatcacc aaccgccgct ggtacgcggt cgtcgtgtga
    63181 agctgaaata tgcccacgct ggtggttata acccgccgat tgtggtgatt cacggtaatc
    63241 aggtgaaaga cctgcctgat tcctacaagc gctacttgat gaactacttc cgcaaatcgc
    63301 tggacgtaat gggatcgccg attcgtattc agttcaaaga aggggaaaac ccgtatgcga
    63361 acaagcgtaa caccctgacg ccaacccaga tgcgtaaacg taagcgtctg atgaagcaca
    63421 tcaagaaaaa taaataatta tccgtaagta ttttatctgc cattccctct atgaatgtag
    63481 agggatggct accggaggaa ttcatatgga actgcattgt ccacaatgtc agcatgtgct
    63541 tgatcaggat aacggccatg cgcgttgccc atcctgcggg gaaattattg aattgaaagc
    63601 tctttgtcca gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta
    63661 cttctgtcag cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta
    63721 atgaaccttt ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca
    63781 gccgtcttcc agacgtgtgg ttagcatttc acccgcttta acttgcttca cttttttcag
    63841 tacattgccg tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct
    63901 tacggcttcg aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtac
    63961 gcgcagggtt tctgctaaac gatattccag ttgctgaatg cgcgtttgcg cgcgatgaat
    64021 cttcggctgt ggattttgct gattcagccg ctgtgttaac cgctgctgct gttgcccggt
    64081 acgcttaagt tgattttcca gcgcaaagct catccgcttt tgcaggcgct caagcatggt
    64141 ttgctggcgt gccagccgga gctgcggatg ctgttgctgt aataggtgat ggatctgcgt
    64201 aaagcgacgc gtgcggttgg cgagataata atccatcgcc atctccagcc gttgacgggt
    64261 cgattgcacc tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga
    64321 cggcgttggc gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc
    64381 gacggcgctg acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt
    64441 aaaactccat aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc
    64501 attgcgctga ttcgccagtt caatggcgcg aacaatttgc cccggtgcgt catcgccctg
    64561 aacggcggtg ggggagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa
    64621 aatatcatgt agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga
    64681 gggaagtggt tttttgtatt gctgatcgaa caaaccttca gcctgcaact tcgctttgag
    64741 ctgttcgtac ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat
    64801 ctggtagtca ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgtcc
    64861 atgctgtgga cggaaggtca cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg
    64921 ggcggtgtcg tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga
    64981 gatttcgccg ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg
    65041 attcaggcga ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc
    65101 aaattctaaa tcagcaggtt attcagtcga tagtaacccg cccttcgggg atagcaagca
    65161 ttttttgcaa aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt
    65221 tattaaccac tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt
    65281 ttgacgacgt tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca
    65341 gcacccagct gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata
    65401 ccgtaacgga agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc
    65461 acaaaaacat gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat
    65521 ctggtgtggt gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag
    65581 aactgaccga gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg
    65641 tgggtattat caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg
    65701 tttacatgac gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg
    65761 tgctggcaaa aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc
    65821 acctgatcgg catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgcct
    65881 gtaaagacga gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg
    65941 aagagcgtgt tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc
    66001 acggtcactc agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc
    66061 tgcaaatcat cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg
    66121 gttgcagcgc ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta
    66181 ctggcgtagg tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca
    66241 ccggtattcc ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta
    66301 tcgccgctgg cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc
    66361 cgggtgaaat cgaactctac cagggccgtt cttacaaatc ttaccgtggt atgggttccc
    66421 tgggcgcgat gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca
    66481 aactggtgcc ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca
    66541 ttcaccagca gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg
    66601 acgaactgcg tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc
    66661 acgttcacga cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct
    66721 cttcgcccga cttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc
    66781 gtcaatgacg gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta
    66841 cactcaactg gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg
    66901 ggatgtgaca gaagcacaaa ttcgtgactt caatccaagc ggcattattc tttccggcgg
    66961 cccggaaagc accaccgaag aaaacagccc gcgtgcgccg cagtatgtct ttgaagcagg
    67021 cgtaccggta ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca
    67081 cgttgaagcc tctaacgaac gtgaatttgg ctacgcgcag gttgaagtcg taaacgacag
    67141 cgcactggtt cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt
    67201 ctggatgagc cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag
    67261 caccgaaagc tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca
    67321 gttccacccg gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg
    67381 tgatatctgc cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc
    67441 tcgcatccgc gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga
    67501 ttcctccgta accgcaatgc tgctgcaccg cgctatcggt aaaaacctga cttgcgtatt
    67561 cgtcgacaac ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga
    67621 tcactttggt cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc
    67681 tggcgaaaac gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt
    67741 cgatgaagaa gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc
    67801 tgacgttatc gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca
    67861 caacgtgggc ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct
    67921 gttcaaagac gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta
    67981 ccgtcatccg ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga
    68041 gtactgtgac ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga
    68101 cctgtacgac aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt
    68161 aatgggcgat ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga
    68221 ctttatgacc gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg
    68281 cattatcaat gaagtgaacg gcatttcccg cgtggtgtat gacatcagcg gcaagccgcc
    68341 agctaccatt gagtgggaat gattttaatc cctgcactat gaatgaacaa aaccctctgt
    68401 tactacagag ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa
    68461 ttaaaccagc tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga
    68521 ccgccttcag tcatatttct gttggcgtca gcttcagatt gcaccacatc ggtttgcgca
    68581 gctttgtgct tcagttcctg atcgataaat tcgttttctc gcttaacgcg ggcttcttct
    68641 tttgccagcg ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt
    68701 tgatcgcgtt ttttgtccgc agaggcttcg gcgtccagtt tatcctgacg agctttctta
    68761 tttgctgctg ccgttgccgc tcttttgtta gcggcggcct gggcatttgc gcgacgttgc
    68821 ttctcttgct ggatttccct gttgcgctcc gcgacccatt catcatgctg cttttgctct
    68881 tcatttttac cttgctgttc cgcttctgcg acagccgaga gttgatcctg caatgatgag
    68941 gcgatagccg gatagcttaa ggaggccaag atggcgcaaa gaaaaacttt cttcatgact
    69001 cctcctgatt attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta
    69061 tacgtcgtgg taataacaac ggctaaacct gtcgtaaact ggcactcttt acccacctgg
    69121 gtggaggtat acactttggt gccttcctta tacgttaaag aaacaccttc cactaaggtt
    69181 ttatcattca ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc
    69241 gtcgttccgg aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg
    69301 ccaataagcg cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact
    69361 tttgcgggaa gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga
    69421 tcggtttgat aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg
    69481 aatgacgcta acattgctac aggcagaaga cattttttaa atttcatcac tattccttgt
    69541 tatacacatt acgaaaaata agacatttaa gatcatcaaa agaggaaagt aaatgtattg
    69601 cgtataaagg gatagattaa attt
//
LOCUS       DAZKHC020000013        65511 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZKHC020000013 DAZKHC020000000
VERSION     DAZKHC020000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 65511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.013"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(31..510)
                     /gene="folK"
                     /locus_tag="ACHOSM_RS05115"
                     /old_locus_tag="pgaptmp_001023"
     CDS             complement(31..510)
                     /gene="folK"
                     /locus_tag="ACHOSM_RS05115"
                     /old_locus_tag="pgaptmp_001023"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGU4557596.1"
                     /translation="MTVAYIVIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAYDKLSKW"
     gene            complement(507..1904)
                     /gene="pcnB"
                     /locus_tag="ACHOSM_RS05120"
                     /old_locus_tag="pgaptmp_001024"
     CDS             complement(507..1904)
                     /gene="pcnB"
                     /locus_tag="ACHOSM_RS05120"
                     /old_locus_tag="pgaptmp_001024"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HGU4557597.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1964..2860)
                     /gene="gluQRS"
                     /locus_tag="ACHOSM_RS05125"
                     /old_locus_tag="pgaptmp_001025"
     CDS             complement(1964..2860)
                     /gene="gluQRS"
                     /locus_tag="ACHOSM_RS05125"
                     /old_locus_tag="pgaptmp_001025"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HGU4557598.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGEPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2927..3382)
                     /gene="dksA"
                     /locus_tag="ACHOSM_RS05130"
                     /old_locus_tag="pgaptmp_001026"
     CDS             complement(2927..3382)
                     /gene="dksA"
                     /locus_tag="ACHOSM_RS05130"
                     /old_locus_tag="pgaptmp_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HGU4557599.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3560..4264)
                     /gene="sfsA"
                     /locus_tag="ACHOSM_RS05135"
                     /old_locus_tag="pgaptmp_001027"
     CDS             complement(3560..4264)
                     /gene="sfsA"
                     /locus_tag="ACHOSM_RS05135"
                     /old_locus_tag="pgaptmp_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HGU4557600.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4279..4809)
                     /gene="thpR"
                     /locus_tag="ACHOSM_RS05140"
                     /old_locus_tag="pgaptmp_001028"
     CDS             complement(4279..4809)
                     /gene="thpR"
                     /locus_tag="ACHOSM_RS05140"
                     /old_locus_tag="pgaptmp_001028"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HGU4557601.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPIAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4883..7312
                     /gene="hrpB"
                     /locus_tag="ACHOSM_RS05145"
                     /old_locus_tag="pgaptmp_001029"
     CDS             4883..7312
                     /gene="hrpB"
                     /locus_tag="ACHOSM_RS05145"
                     /old_locus_tag="pgaptmp_001029"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HGU4557602.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKALLPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7508..10042
                     /gene="mrcB"
                     /locus_tag="ACHOSM_RS05150"
                     /old_locus_tag="pgaptmp_001030"
     CDS             7508..10042
                     /gene="mrcB"
                     /locus_tag="ACHOSM_RS05150"
                     /old_locus_tag="pgaptmp_001030"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HGU4557603.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQNGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWLGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10262..12505
                     /gene="fhuA"
                     /locus_tag="ACHOSM_RS05155"
                     /old_locus_tag="pgaptmp_001031"
     CDS             10262..12505
                     /gene="fhuA"
                     /locus_tag="ACHOSM_RS05155"
                     /old_locus_tag="pgaptmp_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HGU4557604.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTVEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12556..13353
                     /gene="fhuC"
                     /locus_tag="ACHOSM_RS05160"
                     /old_locus_tag="pgaptmp_001032"
     CDS             12556..13353
                     /gene="fhuC"
                     /locus_tag="ACHOSM_RS05160"
                     /old_locus_tag="pgaptmp_001032"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HGU4557605.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDTQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13353..14243
                     /gene="fhuD"
                     /locus_tag="ACHOSM_RS05165"
                     /old_locus_tag="pgaptmp_001033"
     CDS             13353..14243
                     /gene="fhuD"
                     /locus_tag="ACHOSM_RS05165"
                     /old_locus_tag="pgaptmp_001033"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HGU4557606.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAIPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14240..16222
                     /gene="fhuB"
                     /locus_tag="ACHOSM_RS05170"
                     /old_locus_tag="pgaptmp_001034"
     CDS             14240..16222
                     /gene="fhuB"
                     /locus_tag="ACHOSM_RS05170"
                     /old_locus_tag="pgaptmp_001034"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HGU4557607.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISAMLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLIIAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16257..17537)
                     /gene="hemL"
                     /locus_tag="ACHOSM_RS05175"
                     /old_locus_tag="pgaptmp_001035"
     CDS             complement(16257..17537)
                     /gene="hemL"
                     /locus_tag="ACHOSM_RS05175"
                     /old_locus_tag="pgaptmp_001035"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HGU4557608.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17703..17750
                     /gene="yadX"
                     /locus_tag="ACHOSM_RS05180"
                     /old_locus_tag="pgaptmp_001036"
     CDS             17703..17750
                     /gene="yadX"
                     /locus_tag="ACHOSM_RS05180"
                     /old_locus_tag="pgaptmp_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HGU4557609.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17762..19183
                     /gene="clcA"
                     /locus_tag="ACHOSM_RS05185"
                     /old_locus_tag="pgaptmp_001037"
     CDS             17762..19183
                     /gene="clcA"
                     /locus_tag="ACHOSM_RS05185"
                     /old_locus_tag="pgaptmp_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HGU4557610.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWCVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19207..19272
                     /gene="yadW"
                     /locus_tag="ACHOSM_RS05190"
                     /old_locus_tag="pgaptmp_001038"
     CDS             19207..19272
                     /gene="yadW"
                     /locus_tag="ACHOSM_RS05190"
                     /old_locus_tag="pgaptmp_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HGU4557611.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19265..19609
                     /gene="erpA"
                     /locus_tag="ACHOSM_RS05195"
                     /old_locus_tag="pgaptmp_001039"
     CDS             19265..19609
                     /gene="erpA"
                     /locus_tag="ACHOSM_RS05195"
                     /old_locus_tag="pgaptmp_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HGU4557612.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19656..20279)
                     /gene="yadS"
                     /locus_tag="ACHOSM_RS05200"
                     /old_locus_tag="pgaptmp_001040"
     CDS             complement(19656..20279)
                     /gene="yadS"
                     /locus_tag="ACHOSM_RS05200"
                     /old_locus_tag="pgaptmp_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HGU4557613.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20317..21117)
                     /gene="btuF"
                     /locus_tag="ACHOSM_RS05205"
                     /old_locus_tag="pgaptmp_001041"
     CDS             complement(20317..21117)
                     /gene="btuF"
                     /locus_tag="ACHOSM_RS05205"
                     /old_locus_tag="pgaptmp_001041"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HGU4557614.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21110..21808)
                     /gene="mtnN"
                     /locus_tag="ACHOSM_RS05210"
                     /old_locus_tag="pgaptmp_001042"
     CDS             complement(21110..21808)
                     /gene="mtnN"
                     /locus_tag="ACHOSM_RS05210"
                     /old_locus_tag="pgaptmp_001042"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGU4557615.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21892..23409
                     /gene="dgt"
                     /locus_tag="ACHOSM_RS05215"
                     /old_locus_tag="pgaptmp_001043"
     CDS             21892..23409
                     /gene="dgt"
                     /locus_tag="ACHOSM_RS05215"
                     /old_locus_tag="pgaptmp_001043"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HGU4557616.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDHISGMTDLYAWDEYRRLMAVEQ"
     gene            23539..24963
                     /gene="degP"
                     /locus_tag="ACHOSM_RS05220"
                     /old_locus_tag="pgaptmp_001044"
     CDS             23539..24963
                     /gene="degP"
                     /locus_tag="ACHOSM_RS05220"
                     /old_locus_tag="pgaptmp_001044"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HGU4557617.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25118..26275
                     /gene="cdaR"
                     /locus_tag="ACHOSM_RS05225"
                     /old_locus_tag="pgaptmp_001045"
     CDS             25118..26275
                     /gene="cdaR"
                     /locus_tag="ACHOSM_RS05225"
                     /old_locus_tag="pgaptmp_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HGU4557618.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26364..26750)
                     /gene="yaeH"
                     /locus_tag="ACHOSM_RS05230"
                     /old_locus_tag="pgaptmp_001046"
     CDS             complement(26364..26750)
                     /gene="yaeH"
                     /locus_tag="ACHOSM_RS05230"
                     /old_locus_tag="pgaptmp_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HGU4557619.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27065..27889)
                     /gene="dapD"
                     /locus_tag="ACHOSM_RS05235"
                     /old_locus_tag="pgaptmp_001047"
     CDS             complement(27065..27889)
                     /gene="dapD"
                     /locus_tag="ACHOSM_RS05235"
                     /old_locus_tag="pgaptmp_001047"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HGU4557620.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27920..30592)
                     /gene="glnD"
                     /locus_tag="ACHOSM_RS05240"
                     /old_locus_tag="pgaptmp_001048"
     CDS             complement(27920..30592)
                     /gene="glnD"
                     /locus_tag="ACHOSM_RS05240"
                     /old_locus_tag="pgaptmp_001048"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HGU4557621.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30654..31448)
                     /gene="map"
                     /locus_tag="ACHOSM_RS05245"
                     /old_locus_tag="pgaptmp_001049"
     CDS             complement(30654..31448)
                     /gene="map"
                     /locus_tag="ACHOSM_RS05245"
                     /old_locus_tag="pgaptmp_001049"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HGU4557622.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31816..32541
                     /gene="rpsB"
                     /locus_tag="ACHOSM_RS05250"
                     /old_locus_tag="pgaptmp_001050"
     CDS             31816..32541
                     /gene="rpsB"
                     /locus_tag="ACHOSM_RS05250"
                     /old_locus_tag="pgaptmp_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGU4557623.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32799..33650
                     /gene="tsf"
                     /locus_tag="ACHOSM_RS05255"
                     /old_locus_tag="pgaptmp_001051"
     CDS             32799..33650
                     /gene="tsf"
                     /locus_tag="ACHOSM_RS05255"
                     /old_locus_tag="pgaptmp_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGU4557624.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33797..34522
                     /gene="pyrH"
                     /locus_tag="ACHOSM_RS05260"
                     /old_locus_tag="pgaptmp_001052"
     CDS             33797..34522
                     /gene="pyrH"
                     /locus_tag="ACHOSM_RS05260"
                     /old_locus_tag="pgaptmp_001052"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGU4557625.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34814..35371
                     /gene="frr"
                     /locus_tag="ACHOSM_RS05265"
                     /old_locus_tag="pgaptmp_001053"
     CDS             34814..35371
                     /gene="frr"
                     /locus_tag="ACHOSM_RS05265"
                     /old_locus_tag="pgaptmp_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGU4557626.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGVVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35463..36659
                     /gene="ispC"
                     /locus_tag="ACHOSM_RS05270"
                     /old_locus_tag="pgaptmp_001054"
     CDS             35463..36659
                     /gene="ispC"
                     /locus_tag="ACHOSM_RS05270"
                     /old_locus_tag="pgaptmp_001054"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HGU4557627.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTHMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36848..37606
                     /gene="ispU"
                     /locus_tag="ACHOSM_RS05275"
                     /old_locus_tag="pgaptmp_001055"
                     /gene_synonym="uppS"
     CDS             36848..37606
                     /gene="ispU"
                     /locus_tag="ACHOSM_RS05275"
                     /old_locus_tag="pgaptmp_001055"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HGU4557628.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQECIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37619..38476
                     /gene="cdsA"
                     /locus_tag="ACHOSM_RS05280"
                     /old_locus_tag="pgaptmp_001056"
     CDS             37619..38476
                     /gene="cdsA"
                     /locus_tag="ACHOSM_RS05280"
                     /old_locus_tag="pgaptmp_001056"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU4557629.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRLQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38488..39840
                     /gene="rseP"
                     /locus_tag="ACHOSM_RS05285"
                     /old_locus_tag="pgaptmp_001057"
     CDS             38488..39840
                     /gene="rseP"
                     /locus_tag="ACHOSM_RS05285"
                     /old_locus_tag="pgaptmp_001057"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HGU4557630.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39870..42302
                     /gene="bamA"
                     /locus_tag="ACHOSM_RS05290"
                     /old_locus_tag="pgaptmp_001058"
     CDS             39870..42302
                     /gene="bamA"
                     /locus_tag="ACHOSM_RS05290"
                     /old_locus_tag="pgaptmp_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HGU4557631.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPEQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42424..42909
                     /gene="skp"
                     /locus_tag="ACHOSM_RS05295"
                     /old_locus_tag="pgaptmp_001059"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42424..42909
                     /gene="skp"
                     /locus_tag="ACHOSM_RS05295"
                     /old_locus_tag="pgaptmp_001059"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HGU4557632.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42913..43938
                     /gene="lpxD"
                     /locus_tag="ACHOSM_RS05300"
                     /old_locus_tag="pgaptmp_001060"
     CDS             42913..43938
                     /gene="lpxD"
                     /locus_tag="ACHOSM_RS05300"
                     /old_locus_tag="pgaptmp_001060"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HGU4557633.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44044..44499
                     /gene="fabZ"
                     /locus_tag="ACHOSM_RS05305"
                     /old_locus_tag="pgaptmp_001061"
     CDS             44044..44499
                     /gene="fabZ"
                     /locus_tag="ACHOSM_RS05305"
                     /old_locus_tag="pgaptmp_001061"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGU4557634.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44503..45291
                     /gene="lpxA"
                     /locus_tag="ACHOSM_RS05310"
                     /old_locus_tag="pgaptmp_001062"
     CDS             44503..45291
                     /gene="lpxA"
                     /locus_tag="ACHOSM_RS05310"
                     /old_locus_tag="pgaptmp_001062"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HGU4557635.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFSRSTRGLIR"
     gene            45291..46439
                     /gene="lpxB"
                     /locus_tag="ACHOSM_RS05315"
                     /old_locus_tag="pgaptmp_001063"
     CDS             45291..46439
                     /gene="lpxB"
                     /locus_tag="ACHOSM_RS05315"
                     /old_locus_tag="pgaptmp_001063"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HGU4557636.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDFSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46436..47032
                     /gene="rnhB"
                     /locus_tag="ACHOSM_RS05320"
                     /old_locus_tag="pgaptmp_001064"
     CDS             46436..47032
                     /gene="rnhB"
                     /locus_tag="ACHOSM_RS05320"
                     /old_locus_tag="pgaptmp_001064"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGU4557637.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47069..50551
                     /gene="dnaE"
                     /locus_tag="ACHOSM_RS05325"
                     /old_locus_tag="pgaptmp_001065"
     CDS             47069..50551
                     /gene="dnaE"
                     /locus_tag="ACHOSM_RS05325"
                     /old_locus_tag="pgaptmp_001065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGU4557638.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAETRGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHGSLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50564..51523
                     /gene="accA"
                     /locus_tag="ACHOSM_RS05330"
                     /old_locus_tag="pgaptmp_001066"
     CDS             50564..51523
                     /gene="accA"
                     /locus_tag="ACHOSM_RS05330"
                     /old_locus_tag="pgaptmp_001066"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGU4557639.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51622..53763
                     /gene="ldcC"
                     /locus_tag="ACHOSM_RS05335"
                     /old_locus_tag="pgaptmp_001067"
     CDS             51622..53763
                     /gene="ldcC"
                     /locus_tag="ACHOSM_RS05335"
                     /old_locus_tag="pgaptmp_001067"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HGU4557640.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53820..54209
                     /gene="yaeR"
                     /locus_tag="ACHOSM_RS05340"
                     /old_locus_tag="pgaptmp_001068"
     CDS             53820..54209
                     /gene="yaeR"
                     /locus_tag="ACHOSM_RS05340"
                     /old_locus_tag="pgaptmp_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4557641.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54274..55572
                     /gene="tilS"
                     /locus_tag="ACHOSM_RS05345"
                     /old_locus_tag="pgaptmp_001069"
     CDS             54274..55572
                     /gene="tilS"
                     /locus_tag="ACHOSM_RS05345"
                     /old_locus_tag="pgaptmp_001069"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGU4557642.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWFTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55621..55881)
                     /gene="rof"
                     /locus_tag="ACHOSM_RS05350"
                     /old_locus_tag="pgaptmp_001070"
     CDS             complement(55621..55881)
                     /gene="rof"
                     /locus_tag="ACHOSM_RS05350"
                     /old_locus_tag="pgaptmp_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HGU4557643.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55868..56068)
                     /gene="yaeP"
                     /locus_tag="ACHOSM_RS05355"
                     /old_locus_tag="pgaptmp_001071"
     CDS             complement(55868..56068)
                     /gene="yaeP"
                     /locus_tag="ACHOSM_RS05355"
                     /old_locus_tag="pgaptmp_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HGU4557644.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56234..56779
                     /gene="yaeQ"
                     /locus_tag="ACHOSM_RS05360"
                     /old_locus_tag="pgaptmp_001072"
     CDS             56234..56779
                     /gene="yaeQ"
                     /locus_tag="ACHOSM_RS05360"
                     /old_locus_tag="pgaptmp_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HGU4557645.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLKVNLTAWQQPS"
     gene            56776..57198
                     /gene="arfB"
                     /locus_tag="ACHOSM_RS05365"
                     /old_locus_tag="pgaptmp_001073"
     CDS             56776..57198
                     /gene="arfB"
                     /locus_tag="ACHOSM_RS05365"
                     /old_locus_tag="pgaptmp_001073"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HGU4557646.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57212..57922
                     /gene="nlpE"
                     /locus_tag="ACHOSM_RS05370"
                     /old_locus_tag="pgaptmp_001074"
                     /gene_synonym="cutF"
     CDS             57212..57922
                     /gene="nlpE"
                     /locus_tag="ACHOSM_RS05370"
                     /old_locus_tag="pgaptmp_001074"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HGU4557647.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDALSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58077..58900)
                     /gene="yaeF"
                     /locus_tag="ACHOSM_RS05375"
                     /old_locus_tag="pgaptmp_001075"
                     /pseudo
     CDS             complement(58077..58900)
                     /gene="yaeF"
                     /locus_tag="ACHOSM_RS05375"
                     /old_locus_tag="pgaptmp_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(58953..60671)
                     /gene="proS"
                     /locus_tag="ACHOSM_RS05380"
                     /old_locus_tag="pgaptmp_001076"
     CDS             complement(58953..60671)
                     /gene="proS"
                     /locus_tag="ACHOSM_RS05380"
                     /old_locus_tag="pgaptmp_001076"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGU4557648.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(60782..61489)
                     /gene="tsaA"
                     /locus_tag="ACHOSM_RS05385"
                     /old_locus_tag="pgaptmp_001077"
     CDS             complement(60782..61489)
                     /gene="tsaA"
                     /locus_tag="ACHOSM_RS05385"
                     /old_locus_tag="pgaptmp_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HGU4557649.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61486..61890)
                     /gene="rcsF"
                     /locus_tag="ACHOSM_RS05390"
                     /old_locus_tag="pgaptmp_001078"
     CDS             complement(61486..61890)
                     /gene="rcsF"
                     /locus_tag="ACHOSM_RS05390"
                     /old_locus_tag="pgaptmp_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HGU4557650.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62008..62823)
                     /gene="metQ"
                     /locus_tag="ACHOSM_RS05395"
                     /old_locus_tag="pgaptmp_001079"
     CDS             complement(62008..62823)
                     /gene="metQ"
                     /locus_tag="ACHOSM_RS05395"
                     /old_locus_tag="pgaptmp_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HGU4557651.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRSYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62863..63516)
                     /gene="metI"
                     /locus_tag="ACHOSM_RS05400"
                     /old_locus_tag="pgaptmp_001080"
     CDS             complement(62863..63516)
                     /gene="metI"
                     /locus_tag="ACHOSM_RS05400"
                     /old_locus_tag="pgaptmp_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HGU4557652.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKQYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63509..64540)
                     /gene="metN"
                     /locus_tag="ACHOSM_RS05405"
                     /old_locus_tag="pgaptmp_001081"
     CDS             complement(63509..64540)
                     /gene="metN"
                     /locus_tag="ACHOSM_RS05405"
                     /old_locus_tag="pgaptmp_001081"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HGU4557653.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64728..65303
                     /gene="gmhB"
                     /locus_tag="ACHOSM_RS05410"
                     /old_locus_tag="pgaptmp_001082"
     CDS             64728..65303
                     /gene="gmhB"
                     /locus_tag="ACHOSM_RS05410"
                     /old_locus_tag="pgaptmp_001082"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HGU4557654.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15435 a        16304 c        18081 g        15691 t
ORIGIN      
        1 gaaaatattt atattcgtaa tataaataaa ttaccatttg cttaatttgt catatgctct
       61 tgtatgtaag acttcacgca acgtctcccc atcaggaaac gccagctccg gtgcaatctc
      121 aaacagcggc cacagcataa atccacgatt cttcatatcg tagtgcggaa cggtcaggcg
      181 ttcggtattt atcacttcat taccaaacag catgatatcg agatccagcg tgcgtggtcc
      241 ccagcgttca gctttacgaa cacgaccctg ttgcagttct atgcgctgtg tatggttgag
      301 taactcttcc ggggcgagcg aagtttcaag cgccacggcg gcgttgaggt aatcgggttg
      361 atcttgcggc cccagcggtg gggtgcggta aaacgaagaa acggcaagaa tgtggctttc
      421 agggatatcg cctaatgctt tcagggcagc attgacctgc tccagcggag aggccagatt
      481 gctgcctatg acaatatacg ccactgtcat gcggtaccct cacgacgcgg tgcgcgtttg
      541 cgtggacgac gagtacggcg acgcggtgac ggctcttcat ccaactcgtt aagcatccct
      601 ttttgatctg gtggcgcgga aacctggaac tcaccccacc atttcaccag acgctgcagt
      661 tcagcgttac gttcaacttc agctcgcaag gccaacaggt cataagccgc acggaactta
      721 ggatgctcca gcagtttcca tgcgcgttta ccctgacgac gggacatacg caactgcaac
      781 tgccagatat cgcgggttaa cgtcgtcaga cgtttcggga ttgccagtga acggcaggct
      841 tcgtccagca cgtcgttcat cgccagcgcg aaagcgtcgt gataggtcag gccgctttcc
      901 tgggcaatct tctgtgccgt ctccagcagt gggtaccaga acatggcggc aaacaggaac
      961 gccgggttca cgcgcatatc gttatggata cgcgtatcgg tattcttcag cacctgttca
     1021 atgatccgct ccatcgggct gtcgccattt tccgtgaagt agcgggtaat ggtcgggaac
     1081 agcggctgga acagatgata ttcacacaac agcttatagg tatcgtaacc gtagcccgct
     1141 tgtagcagtt taagcgattc ttcaaacagg cgtgccggtg ggatatcgtt caacagggta
     1201 gcgaggcgag ggatcggttc tgcggtttcc gggctgatgc gcatacccaa tttggcggca
     1261 aaacgtaccg cgcgcagcat acgtaccgga tcttcacggt agcgcgtttc cgggttacca
     1321 atcagacgga taacgccgtc cttcagatcc ttcataccgc caacgtaatc acggacggta
     1381 aaatccgcta cgctgtaata gaggctgttg atagtgaaat cgcggcgctg ggcgtcttct
     1441 tcgatggagc cgaaaatgtt gtcgcgcagc aacatgccgt tttgcccgcg ttgggaggtc
     1501 gtgcggtcgc tgacgttacc ttcgtggtgt ccacggaagg tcgcaacttc gataatctcc
     1561 gggccaaaca ttacatgagc cagacggaaa cggcgaccca ccaggcggca gttacggaac
     1621 agtttgcgca cctgctcagg cgtggcgtta gtggttacgt caaaatcttt cggctttttg
     1681 ccaagtaaca ggtcgcgcac gccgccgcca accagccagg cttcgtatcc cgctttgttg
     1741 agcctgtaca ttaccttcag ggcattttca ctgatatctt tgcgggaaat agcatgctgc
     1801 tcacgcggga tcaccgtcac ctgtggacgg gcgactgcct gttcagcctc gctttcctcg
     1861 cggcttagca ccttgcggca aaaattagcg actcgggtaa aaatagtaca cctcggtagt
     1921 gtcaaacatc attcaggaca aaaaaatagc ggctaatcat agctcagcat gacgcatttg
     1981 agaatgttga atttacaatt gccgactcgg gcacggcggt cagcgtccag tttttgacgg
     2041 ctgactgaag gatttgctcg acgctgaaat cctgccagtg tgcttctgcc tgctgcccca
     2101 gaaattgaag tgccgcgatt agtaccgggc gtggttcgcc tttcggcaac gcaggcgcat
     2161 gattctgctt ggaaagttta gcgccttgtg gattaagcgc cagcggcaga tgaatgtaat
     2221 ctggcacttt ccagccaaaa agctggtaca gcgagatttg ccttactgtt ggttcaatca
     2281 gatcagcccc acgcactatt tctgtaacgc cctggaaatg atcatcaacc acaacagcca
     2341 ggttgtaggc gaacaaccca tcacggcgat gaatgataaa atcttcccgt gccagttttt
     2401 cgtcggcgtg aataatgccg cgcagctggt cagtaaattg cgtgaccgga tgctgctggc
     2461 ggatacgcac tgcggcattg tctggtccat gatgcaacac ccggcaatga ccgtcgtaaa
     2521 taccgccaat gctttgaata cgcgcacgcg tacaggtgca gtaataactt agtccttgtt
     2581 catgtaacca ggcgagtgct tcacgatagg cgtcgtgacg ttgcgattgc cagagaacat
     2641 cgccgtccca gtgcagaccg taatgttcca gctggcgcag gatagtttct gcggcaccgg
     2701 gaacttcacg aggcgggtcg atatcttcta tgcgtaccag ccagcgacct tgccgggcgc
     2761 gagcctgcaa atagctgccg agcgcggcga tcagagagcc aaaatgaagc tcgccggaag
     2821 gagagggggc gaagcggcca atatactgtg tgtctgtcat ctctttgaac aaaaaataag
     2881 gcgggagcat ttcccgcctg tggtaaacgt gatggaacgg ctgtaattag ccagccatct
     2941 gtttttcgcg aatttcagcc agcgttttgc agtcgatgca cagatcggct gtcgggcgcg
     3001 cttccagacg gcgaatacca atttcaacac cgcaggattc gcagtagccg aaatcttcgt
     3061 cttccacttt tttcagcgtc ttctcgatct ttttgatcag cttacgctcg cgatcgcggt
     3121 tacgcagttc gaggctgaac tcttcttcct gggctgcacg gtctaccggg tccgggaagt
     3181 tggctgcttc atcctgcata tgtgtaacgg tgcgatcgac ttcatccctg agttgattac
     3241 gccatgcttc cagaatacga cggaagtgcg ccagctgggc ttcattcata tactcttcgc
     3301 ccggcttctc ctgatatggt tccaccccag cgatggcgag aatactcagg gacgatgttt
     3361 tacggttttg cccttcttgc atgttgcttc tccttaacac gcactatcga tccccatgtt
     3421 cgggggaaaa atgaggccgc tataaatagc agatgctttt ccggatagca attctctaaa
     3481 cgtaacactt gacaactgtg tgaggaaaag cgtatttgcg cacgcgacca gaatgtaaat
     3541 taaccagtta cttactttac tacaatgtaa ccggcagtga ttttttaaga gccatgcctt
     3601 cagcagaaat ttccgctttg taagccagaa tttctacccc cctctgttga gcttctgaca
     3661 atagttgcgc gtatttctca tcgatgtggc gcgcgggtga aaaccgtgta atggctgaat
     3721 gcagcacggc gaaaaagata accgcacgct ggccttcagc cgctacgctc atcaactccc
     3781 gaaggtgttt ctgacctcgt tcagtgaccg catcgggaaa atatccctgt tcgttctccg
     3841 ctaacgtaac cgatttcact tcaatatagc agtctggacg cgaatccgcc tgcaacataa
     3901 agtcaatacg gctgcgttct gcgccgtatt ttacttcgct tttcagcgag ctatagcctg
     3961 acagttctga aattgattca ttaaggatag cctctttcgt caacctgtta gcccaaagcg
     4021 tgttgacgca aataaatgcg ccgctctggc tttgagttaa ttcccaggtg tgtgggtatt
     4081 tccgtttggt gttgtctgaa gtcgaatacc agacggtatc gccaggcgtt gcacaaccgg
     4141 tcattgcacc cgtattcggg cagtgtagcg ttaattcgcg accatcgggt gtgatcacat
     4201 cggctaaaaa acgtttgtaa cgctgaatta gcgtcgcgcg ctgtagaggg ggagagaatt
     4261 ccattgcgac aatccttgtt attgcgttag cgcccagcgt tttagcggcg tgtagcgtgt
     4321 gcgtccacgg gcaaacgagg aggcgtaaag ggtgaactcc gtcaccgcat aagaccagtt
     4381 aaaacctggc ggcgggattg tcaccgcctc gctggcgtcg cgcaataagg taatatgtgg
     4441 atgaaacgga cgattgcttt gaaaacaacc gctgcgggca gcctgtgaac ggagcatatt
     4501 cgccagctgg attaagccgc gtggaggctg acgcatccct aaccacacca cacgcgaacg
     4561 cagccattgt ccggcgtcat caagcgtgag tgtgaaacca ggttgacgaa tccgtccggc
     4621 taaaagagaa agcgccttct ctttctcggc gctcacttcg cctaaaaatg ccagagttag
     4681 atgcaaatta tcggcggcga tcggacgtcc cgcctcaggt gggaagtgtg tggcgcgcca
     4741 atggataatc tgttcgcgga tttctgcagg taagtcgata gcaaagaaca gacgttgcgg
     4801 ttcagacatg tgaggcactc ggttatgaat taccacgatg ctacaatgtg gcgcgaagaa
     4861 tgttaaccct ctggagcgtt ttgtgtcgtc gttgcccgtt gctgccgtct tatctgaatt
     4921 acttaccgct ctcgattgtg cgccgcaggt attattaagt gcgccgaccg gggccgggaa
     4981 atcaacctgg ctgccgctgc aactgctggc gcatcccggc attaacggga aaattatcct
     5041 gctggagccg cgtcgtctgg cggcgcgtaa cgtcgcgcaa cggctggcgg agctgcttaa
     5101 cgaaaagcca ggcgataccg ttggctaccg gatgcgtgcg caaaactgcg tcgggccgaa
     5161 tacccgcctg gaagtagtta ccgaaggcgt gctgacgcgc atgatccagc gtgacccgga
     5221 actgagcggt gttggattgg tgatcctcga tgagtttcat gagcgtagct tgcaggcgga
     5281 tttggcgttg gcgctgttac tcgatgtgca acaaggtctg cgtgatgacc ttaaactgct
     5341 aattatgtcg gctacgctgg acaacgaccg cttgcagcaa atgctgccag aagcgcccgt
     5401 cgtcatctca gaagggcgct cgtttccggt tgaacgccgt tatttaccgc tgcccgcgca
     5461 tcagcgtttt gacgatgccg ttgcggtagc caccgctgaa atgctgcgtc aggaaagcgg
     5521 atcattactg ttatttttac ctggcgtcgg agaaattcag cgtgtgcagg aacaactggc
     5581 ttcgcgcatc ggcagtgatg tattgctctg cccgctgtat ggcgcgttgt cgctgaacga
     5641 tcagcgaaaa gcgctcctcc cggcaccgca agggatgcgc aaagtggtgc tggcgaccaa
     5701 tattgctgaa accagtttaa ccattgaagg tattcgtctg gtggtggatt gtgcccagga
     5761 gcgtgtggcg cgttttgatc cgcgcacggg gcttacgcga ctgattactc aacgcgttag
     5821 ccaggcatcc atgacgcagc gtgccgggcg cgccgggcgt ctggagccgg gtatctgcct
     5881 gcatttaatc gccaaggaac aagcagaacg cgctgccgcg caaagtgaac cggaaatctt
     5941 acaaagcgat ctttccggtt tgctgatgga attactgcaa tggggatgca gcgatccggc
     6001 gcagatgagc tggctggatc aaccgccaac ggtgaatcta ctggccgcga aacgcctgtt
     6061 acaaatgctg ggggcgctgg agggtgaacg gcttagtgcg caggggcaaa aaatggcagc
     6121 gctgggtaac gatccgcgtt tagcggcaat gctggttagc gcgaagaacg acgacgaagc
     6181 tgctaccgcg gcaaaaattg ccgccattct cgaagagccg ccacggatgg gcaatagtga
     6241 cctgggcgtg gcgttttcgc gcaatcaacc agcctggcag caacgtagtc agcaactgtt
     6301 aaaacgctta aacgtacgtg gcggtgaggc agacagttcg cttatcgcgc cgctacttgc
     6361 cggggcgttt gccgatcgca ttgctcgtcg ccgtgggcaa gatggacgct atcaactggc
     6421 aaacggcatg ggagcgatgc tcgatgccaa cgacgcgcta agccgccacg aatggttgat
     6481 cgcaccgtta ttattgcagg gcagcgcctc gccggatgcg cggattttac tggcgctgcc
     6541 ggtcgatatt gatgagttag tacaacgctg cccgcagctg gtacagcagt ctgacactgt
     6601 ggagtgggat gacgcgcaag gtacgctgaa agcctggcgt cggctgcaaa tcggtctgct
     6661 gacggtgaaa gtgcagccgc tggcgaaacc ctcggaagac gagttgcatc aggcgatgct
     6721 taatggcatc cgcgataaag gtttaagcgt gctcaactgg acggcggaag cggaacagct
     6781 acgcttgcgt ttgttatgcg ccgcaaagtg gttgccggaa tatgactggc cagcggttga
     6841 tgatgaaagt ttattggcga cgctggaaac gtggctgctg ccacatatga ctggcgtaca
     6901 ttcactacgc ggcctgaaat cactcgacat ttatcaggca ctacgcggat tacttgattg
     6961 gggaatgcag caacgtctgg atagtgaatt gcctgcgcat tacactgtgc cgacgggaag
     7021 ccggatcgcc attcgttatc atgaagataa cccgcccgcg ctggcggtga gaatgcaaga
     7081 gatgtttggc gaggccacca atccgacgat cgcccagggg cgcgtgccgc tggtgctgga
     7141 gttgctttca cctgcccaaa ggccattaca aatcacacga gatttgagcg ccttctggaa
     7201 aggagcgtac cgtgaggtgc aaaaagagat gaaagggcgt tatcccaaac atgtctggcc
     7261 ggacgacccg gcaaatactg caccgacgcg acggacgaaa aagtattcgt aaggttggtt
     7321 ttctccctct ccctgtggga gagggccggg gtgagggcat cagcgcgcac gttcaccctc
     7381 accctaaccc tctccctcaa gggagagggg accgatcgag cacaaatttt gagagatatc
     7441 ttcttctgtc ttgtaacaga agaacagaaa atcgggcttt tgcgcctgaa tattgcggag
     7501 aaaaagcatg gccgggaatg accgcgagcc aattggacgc aaagggaaac cgacgcgtcc
     7561 ggtcaaacaa aaggtaagcc gtcgtcgtta cgaagatgac gatgattacg acgattatga
     7621 tgactatgag gatgaagaac cgatgccgcg caaaggtaag ggcaaaggca aagggcgtaa
     7681 gcctcgtggc aaacgcggct ggctatggct gctgttaaaa ctggctatcg tttttgccgt
     7741 gctgatcgcc atttacggcg tttatctcga tcaaaaaatt cgtagccgta ttgatggcaa
     7801 ggtctggcaa ctgcctgcgg cagtttatgg ccgaatggtc aatcttgagc cagatatgac
     7861 catcagcaag aacgagatgg tgaagctgct ggaggcgact cagtatcgtc aggtgtcgaa
     7921 aatgacccgt cctggcgaat ttaccgtgca ggccaacagc attgagatga tccgccgtcc
     7981 gtttgatttc ccggacagta aagaagggca ggtgcgtgcg cgtctgacct ttgatggtga
     8041 tcatctggcg acgatcgtca atatggagaa caaccgtcag ttcggctttt tccgtcttga
     8101 tccgcgtctg atcaccatga tctcctcgcc aaacggtgag caacgtctgt ttgtgccgcg
     8161 cagtggtttc cctgatttgc tggtggatac tttgctggcg acagaagacc gccatttcta
     8221 cgagcatgat gggatcagcc tctactcaat tgggcgtgcg gtgctggcaa acctgaccgc
     8281 aggtcgcacg gtgcagggtg cgagtacgct gacgcaacag ctggtgaaaa acctgttcct
     8341 ctccagcgag cgttcttact ggcgtaaagc gaacgaagct tacatggcgc tgatcatgga
     8401 cgcgcgttac agcaaagacc gtattcttga gctgtatatg aacgaggtgt atctcggtca
     8461 gaacggcgac aacgaaatcc gcggcttccc gctggcgagc ttgtattact ttggtcgccc
     8521 ggtagaagag ctaagcctcg atcagcaggc actgttagtc ggtatggtga aaggagcgtc
     8581 catctacaac ccgtggcgta acccgaaact ggcgctggag cgacgtaatc tggtgctgcg
     8641 tctgctgcaa cagcaacaga ttattgatca agaactctat gacatgttga gtgcacgtcc
     8701 gctgggggtt cagccgcgcg gtggggtgat ctctccacag ccagccttta tgcaactggt
     8761 gcgtcaggag ctgcaggcaa aactgggcga taaggtaaaa gatctctccg gcgtgaagat
     8821 cttcactacc tttgactcgg tggcccagga cgcggcagaa aaagccgccg tggaaggcat
     8881 tccggcactg aagaaacagc gtaagttgag cgatcttgaa actgcgattg tggtcgtcga
     8941 ccgctttagt ggtgaagttc gtgcgatggt cggaggttct gagccgcagt ttgcgggcta
     9001 caaccgtgcg atgcaggcgc gtcgttcgat tggttccctt gcaaaaccag cgacttatct
     9061 gacggcttta agccagccga aaatctatcg tctgaatacg tggattgcgg atgcgccaat
     9121 cgcgctgcgt cagccgaatg gccaggtctg gtcaccgcag aacgatgatc gtcgttatag
     9181 cgaaagtggc agagtgatgc tggtggatgc gttgacccgt tcgatgaacg tgccgacggt
     9241 aaatctgggg atggcgctgg ggctgcctgc ggttacggat acctggatca aactgggcgt
     9301 gccgaaagat cagctgcatc cggttccggc aatgctgctg ggggcgttga acttaacgcc
     9361 aatcgaagtg gcgcaggcat tccagaccat cgccagcggt ggtaaccgtg caccgctttc
     9421 tgcgctgcgt tcggtaatcg cggaagatgg caaggtgttg tatcagagct tcccgcaggc
     9481 ggaacgcgct gttccggcgc aggcggcgta tctgacacta tggaccatgc agcaggtggt
     9541 acaacgtggt acgggtcgtc agcttggggc gaaatacccg aacctgcatc tggcagggaa
     9601 aacagggact accaacaata acgtagatac ctggtttgca ggcattgacg gcagcacggt
     9661 gaccatcacc tggctaggcc gtgataacaa ccagccgacc aaactgtatg gagccagcgg
     9721 ggcaatgtcg atttatcagc gttatctggc taaccagacg ccaacgccgc tgaatcttgt
     9781 tccgccagaa gatattgcag atatgggcgt ggactacgac ggcaactttg tttgcagcgg
     9841 tggcatgcgt gtcttgccgg tctggaccag cgatccgcaa tcgctgtgcc agcagagcga
     9901 gatgcagcag cagccgtcag gcaatccgtt tgatcagtct tctcagccgc agcaacagcc
     9961 gcaacagcaa cctgctcagc aagagcagaa agacagcgac ggtgtagccg gttggatcaa
    10021 ggatatgttt ggtagtaatt aacatctaag cgtgaaatac cggatggcga gttgccatcc
    10081 ggtaaaataa catcccatct aagatattaa ccctttcttt tcatctggtt gtttattaac
    10141 ccttcaggaa cgctcagatt gcgtaccgct tgcgaacccg ccagcgtttc gaatattatc
    10201 ttatctttat aataatcatt ctcgtttacg ttatcattca ctttacatca gagatatacc
    10261 aatggcgcgt tccaaaactg ctcagccaaa acactcactg cgtaaaatcg cagttgtagt
    10321 agccacagcg gttagcggca tgtctgttta tgcacaggca gcggttgaac cgaaagaaga
    10381 cactatcacc gttaccgctg cacctgcgcc gcaagaaagc gcatgggggc ctgctgcaac
    10441 tattgcggcg cgacagtcag ctaccggcac taaaaccgat acgccgattc aaaaagtgcc
    10501 acagtctatt tctgttgtga ccgtcgaaga gatggcgctg catcagccga agtcggtaaa
    10561 agaagcgctt agctacacgc cgggtgtctc tgttggtacg cgtggcgcat ccaacaccta
    10621 tgaccacctg atcattcgcg gttttgcggc agaaggccaa agccaaaata actatctgaa
    10681 tggcctgaag ttgcagggca atttctataa cgatgcggtc attgacccgt atatgctgga
    10741 acgcgctgaa attatgcgtg gcccggtttc cgtgctttac ggtaaaagca gtcctggcgg
    10801 tctgttgaat atggtcagca agcgtccgac caccgaaccg ctgaaagaag ttcagtttaa
    10861 agccggtact gacagcctgt tccagactgg ttttgacttt agcgatgcgt tggatgatga
    10921 cggtgtttac tcttatcgcc tgaccggtct tgcgcgttct gccaatgccc agcagaaagg
    10981 gtcagaagag cagcgttatg ctattgcacc ggcgttcacc tggcgtccgg atgataaaac
    11041 caattttacc ttcctttctt acttccagaa cgagccggaa accggttatt acggctggtt
    11101 gccgaaagag ggaaccgttg agccgctgcc gaacggtaag cgtctgccga cagactttaa
    11161 tgaaggggcg aagaacaaca cctattctcg taatgagaag atggtcggct acagcttcga
    11221 tcacgaattt aacgacacct ttactgtgcg tcagaacctg cgctttgctg aaaacaaaac
    11281 ctcgcaaaac agcgtttatg gttacggcgt ctgctccgat ccggcgaatg cttacagcaa
    11341 acagtgtgcg gcattagcgc cagcggataa aggccattat ctggcacgta aatacgtcgt
    11401 tgatgatgag aagctgcaaa acttctccgt tgatacccag ttgcagagca agtttgccac
    11461 tggcgatatc gaccacaccc tgctgaccgg tgtcgacttt atgcgtatgc gtaatgacat
    11521 caacgcctgg tttggttacg acgactctgt gccactgctc aatctgtaca atccggtgaa
    11581 taccgatttc gacttcaatg ccaaagatcc ggcaaactcc ggcccttacc gcattctgaa
    11641 taaacagaaa caaacgggcg tttatgttca ggatcaggcg cagtgggata aagtgctggt
    11701 caccctagga ggtcgttatg actgggcaga tcaagaatct cttaaccgcg ttgctgggac
    11761 gaccgataaa cgtgatgaca aacagtttac ctggcgtggt ggtgttaact acctgtttga
    11821 taatggtgta acaccttact tcagctatag cgaatcgttt gaaccttctt cgcaagttgg
    11881 gaaggatggt aatattttcg caccgtctaa aggtaagcag tatgaagtcg gcgtgaaata
    11941 tgtaccggaa gatcgtccga ttgtagttac tggtgccgtg tataatctca ctaaaaccaa
    12001 caacctgatg gcggaccctg agggttcctt cttctcggtt gaaggtggcg agatccgcgc
    12061 acgtggcgta gaaatcgaag cgaaagcggc gctgtcggcg agtgttaacg tagtcggttc
    12121 ttatacttac accgatgcgg aatacaccac cgatactacc tataaaggca atacgcctgc
    12181 acaggtgcca aaacacatgg cttcgctgtg ggctgactat accttctttg acggtccgct
    12241 ttcaggtctg acgctgggca ccggtggtcg ttatactggc tccagctatg gtgatccggc
    12301 taactccttt aaagtgggaa gttatacggt cgtggatgcg ttagtgcgtt atgatctggc
    12361 gcgagtcggc atggcgggct ccaacgtggc gctgcatgtt aacaacctgt tcgatcgtga
    12421 atacgtcgcc agctgcttta acacttatgg ctgcttctgg ggcgcagaac gtcaggtcgt
    12481 tgcaaccgca accttccgtt tctaatttct cttttggggc acggatttcc gtgcccattt
    12541 cacaagttgg ctgttatgca ggaatacacg aatcattccg ataccacttt tgcactgcgt
    12601 aatatctcct ttcgtgtgcc cgggcgcacg cttttgcatc cgctgtcgtt aacctttcct
    12661 gccgggaaag tgaccggtct gattggtcac aacggttctg gtaaatccac tctgctcaaa
    12721 atgcttggcc gtcatcagcc tccgtcagaa ggggagattc ttcttgatac ccagccgctg
    12781 gaaagctgga gcagcaaagc gtttgcccgc aaagtggctt atttgccgca gcagcttcct
    12841 ccggcagaag ggatgaccgt gcgtgaactg gtggcgattg gtcgttaccc gtggcatggc
    12901 gcgctggggc gctttggtgc ggcagatcgc gaaaaggtcg aggaagctat ctcgctggtt
    12961 ggcttaaaac cgctggcgca tcggctggtc gatagtctct ctggcggcga acgtcagcgg
    13021 gcgtggatcg ccatgctggt ggcgcaggat agccgttgtc tgttgctcga cgaaccgact
    13081 tcggcgctgg atatcgccca ccaggttgat gtgctggcgc tggtgcaccg tttaagtcag
    13141 gaacgtggcc tgacggtcat tgccgtgctg cacgatatca atatggcggc acgctactgt
    13201 gattatctgg tcgccctgcg cggtggtgaa atgattgctc agggaacgcc tgcggaaatt
    13261 atgcgcggcg aaaccctcga aatgatttat ggcatcccga tgggtatttt gccgcatccg
    13321 gcgggtgctg cacctgtgag ttttgtttat tgatgagcgg cttacctctt atttcgcgcc
    13381 gtcgactgtt aacggcgatg gcgctttctc cgttgttatg gcagatgaat accgcccacg
    13441 cggcggctat tgaccccaat cgtattgtgg cgctggagtg gttgccggtg gaattactgc
    13501 tggcgctcgg catcgtgcct tacggcgtgg cggataccat caactatcgc ctgtgggtca
    13561 gcgaaccacc attgccggac tcagtgattg acgtcggttt gcgtacagaa cctaaccttg
    13621 aactgctgac cgaaatgaaa ccgtcgttta tggtctggtc ggcaggatat ggcccttcat
    13681 cagaaatgct ggcacgtatt gcgccgggtc gcggatttaa cttcagtgac ggcaaacagc
    13741 cgttggcgat ggcgcgtaaa tcgctgacgg aaatggcaga tttacttaac ctgcaaagcg
    13801 cagcggaaac gcatttagcg caatacgaag actttatccg cagcatgaaa ccccgctttg
    13861 tgaagcgtgg tgcacgcccg ttactgctga cgacgcttat cgatccgcgc catatgctgg
    13921 tcttcggtcc aaacagcttg ttccaggaaa ttctcgatga gtacggcatc ccaaatgcct
    13981 ggcaagggga aaccaacttc tggggcagta ccgccgtcag tatcgatcgt ctggcggcgt
    14041 ataaagacgt tgatgtgctc tgttttgatc acgacaacag caaagacatg gatgcgctaa
    14101 tggcaacccc gctgtggcag gccataccgt ttgtccgcgc cggacgcttt cagcgcgtac
    14161 ctgcagtctg gttttatggt gcgacgctct cggcaatgca ctttgtgcgc gttctggata
    14221 acgccatcgg aggtaaagcg tgagtaaacg aattgcgctt tttccggcgt tattgctggc
    14281 gctgttagtg attgtcgcta cggcgctcac ctggatgaac ttctcgcagg cgctgccgcg
    14341 tagccagtgg gcgcaggctg cctggtcgcc agacatcgat gtcatcgagc agatgatttt
    14401 tcactacagc ttgttgccgc gtctggcgat ttcgctgctg gtgggcgcgg gcctggggct
    14461 ggtgggcgtg ctgtttcagc aagtgctgcg taacccgctg gcggagccga cgacgcttgg
    14521 cgttgctaca ggcgcgcaac tgggaattac cgtcaccaca ctttgggcga tcccaggtgc
    14581 tatggcgagc cagtttgccg cgctggtggg cgcttgtgtc gttggcttaa tcgtctttgg
    14641 cgtcgcgtgg gggaaacggc tttcgccggt tacgcttatt ctcgcggggc tggtggtgag
    14701 cctttattgc ggggcaatta atcagctact ggttatcttc catcacgacc agctgcaaag
    14761 catgttcctg tggagcaccg gaacgctgac gcaaaccgac tggggcggcg ttgagcgttt
    14821 atggccgcag ttgctgggcg gcgtgatgtt gacgttattg ctacttcgcc cgttaaccct
    14881 gatggggctt gatgatggcg tggcgcgcaa tctcgggctg gccttatcgc ttgcgcgtct
    14941 ggcggcgctg tcgctggcga tagtcatcag tgcgatgctg gtgaatgcgg tggggattat
    15001 cggctttatc ggtttgttcg cgccgctgct agcaaaaatg ctgggggcgc ggcgtttgtt
    15061 gccacggttg atgctggcgt cgctgattgg ggcgctgatc ctctggcttt ccgatcaaat
    15121 catcctctgg ctgactcgcg tgtggatgga agtttccacc ggttcggtga cggcgttgat
    15181 aggtgcgccg ctgttactgt ggctgctgcc gcgtttacgc agcatcagcg cgccggatat
    15241 gaaggtcaac gatcgtgtcg cggctgaacg ccaacatgtg ctggcgtttg cccttgctgg
    15301 cggtgtgctg ctgataattg ctgtggtggt ggcgctgtcg tttggtcgtg atgcgcacgg
    15361 ctggacgtgg gcgagcgggg cgttgctcga ggatttaatg ccctggcgct ggccgcgaat
    15421 tatggcggcg ctgtttgcgg gcgtcatgct ggcggtggcg ggctgtatta ttcagcggct
    15481 gaccggaaac ccgatggcaa gcccggaagt gctggggatt agctctggcg cggcgtttgg
    15541 cgtggtgttg atgctgtttc tggtgccggg taatgccttt ggctggctgt tacctgcagg
    15601 cagtctcggc gcggcggtga cgctgttgat cattatgatc gccgccggcc gcggtggatt
    15661 ttccccacac cgcatgttac tggcggggat ggcgttaagc accgcgttca ccatgctttt
    15721 gatgatgttg caggcaagtg gtgacccgcg aatggcgcaa gtgctgacct ggatttccgg
    15781 ttcgacctac aacgcgaccg atgcgcaggt ctggcgcacc ggaattgtga tggtgatttt
    15841 gctggcgatt accccgctgt gccgccgctg gctgaccatt ttaccgctgg gtggtgatac
    15901 cgcccgagcc gtaggaatgg cgctgacgcc gacgcgaatt gcgctgctgc tgttagcggc
    15961 ttgcctgacg gcgaccgcga cgatgactat tggaccgttg agttttgttg gtttaatggc
    16021 accgcatatt gcgcggatga tgggctttcg acggacgatg ccacacatcg taatttcggc
    16081 gctggtgggt ggtttactgc tggtgttcgc tgactggtgt gggcggatgg tgctgtttcc
    16141 attccagatc ccggcggggc tgctgtcgac ctttatcggt gcgccatatt ttatctattt
    16201 gctgagaaag cagagccgtt aatagaatgc ctcgcccggt gggcgaggca aaattatcac
    16261 aacttcgcaa acacccgacg tgcagcatcg atggtgttat tgatatcttc catgctgtgc
    16321 gccacggaca taaagcccgc ttcaaacgct gacggtgcca ggtaaacacc ttcgtccagc
    16381 atcatatgga agaaacgctt aaagcgttcc acgtcacagg ccatcacatc ctgatagcac
    16441 gtcacggact cggcgtcggt aaagaaaata ccgaacatgc cgccaacgtg gttaacgacc
    16501 agcggaattc cggcttcttc tgctgcttcc agcagacctt ctgccagacg tgatgtcagc
    16561 tcatccaacg tttcgtgaac gcccggctgc gcgacttcat tcagacaggc gaaaccggct
    16621 gccatcgcaa ttgggttacc ggaaagcgta cccgcctgat agaccggacc cgtcggggcc
    16681 agcgcatcca ttacatcacg acgaccaccg aatgcgccta ccggcattcc accgccgatg
    16741 attttgccca ggcaggtgag atccggttcc acaccgtaat aatcctgtgc gccagctagc
    16801 gctacgcgga agccagtcat tacttcatcg atgatcagca atgcgccaaa ttcgtcgcac
    16861 agcgcgcgca gacctggcag gaactctggc agcggtggaa cgcagttcat attgcctgcc
    16921 accggctcga cgataataca ggcaatctct tgcgggtatt gctcaaacgc ggcgcgtaca
    16981 gaagccagat cgttataagt acaggttaag gtatgtttgg cgaaatctgc cggaacaccc
    17041 ggcgagtttg gctggcctaa cgtgagtgcg ccagaaccgg ctttcaccag caggcagtca
    17101 gcgtgaccgt ggtaacaacc ttcaaattta atgattttgt cgcgaccggt aaaaccacgg
    17161 gccaggcgga tggcgctcat cgtcgcttca gtgccggagt tcaccatgcg caccatatcc
    17221 atggtcggga ccagttcggt caccagttgc gccattttca cttccatttc ggtcggtgca
    17281 ccaaagctta aaccacgctc ggcggcttca atcactgcat tgcggatcgc cggatggtta
    17341 tggcccagca ccatcggccc ccaggaaccg acataatcga tataggcttt gccatcaaca
    17401 tcgtacagat aagcaccgtc cgctttttcg ataaacagtg gagtgccgcc cacgccagta
    17461 aaggcgcgaa caggggagtt cacaccgcca gggatcagct cgcgcgctgc gctgtaaaga
    17521 ttttcagact tactcatgga gggttcctga ttcgtagaaa aagtgaatgg ctgctattct
    17581 atgttattca taacaagtta aatactcgtc aaacatcagg ctgcttgtac tggtgcaatc
    17641 ctgatttctt agagtataaa agttttgtgc atttgaaaca ttacgctttg caaaggattt
    17701 tcatggaacg tgcgagtaaa atgccgtcat cttatttgta tgaccaataa gtgatcattg
    17761 gatgaaaact gatactccct ctttagaaac accgcaggcc gcgcgcctgc gacgcagaca
    17821 actgattcgc caacttcttg agcgcgataa aaccccgtta gccattttgt ttatggcggc
    17881 agtcgtcggc acgcttgtcg ggctggctgc ggttgctttt gacaaaggtg tcgcctggtt
    17941 gcagaaccaa cgtatggggg cgctggtaca tactgctgat aattatccgc tactgttaac
    18001 cgtcgctttt ctctgttcgg cggtgctggc gatgttcggc tactttttgg tgcgcaaata
    18061 cgcgcccgaa gcagggggat cggggatccc ggaaattgaa ggggcgctgg aagatcaacg
    18121 tcctgttcgc tggtggtgtg tattgccggt gaaattcttt ggcgggctgg ggacactcgg
    18181 cggaggcatg gtgctagggc gcgaaggacc aaccgtgcag atcggcggca atatcggtcg
    18241 catggtgctt gatattttcc gcctgaaagg tgatgaagct cgccatacgc tgctggcaac
    18301 cggtgctgct gcggggctgg ctgcggcctt taacgcgccg ctggcgggta ttttgtttat
    18361 tatcgaagag atgcgtccgc agtttcgcta tacgttaatt tcgattaaag cggtatttat
    18421 tggcgtcatt atgtcgacca ttatgtaccg gatttttaat catgaagttg cgttgattga
    18481 cgtcggtaaa ctttctgatg cgccgcttaa tacgctgtgg ctttatctga tcctcggtat
    18541 tatttttggc attttcggcc ctatttttaa taaatgggtg ctggggatgc aggatttgct
    18601 gcaccgtgtg cacggcggca atattaccaa atgggtactg atgggtggtg cgattggcgg
    18661 tctgtgtgga ttgctggggt ttgtggcacc agcaacgtcg ggcggcggtt ttaacctgat
    18721 tcctatcgct accgcgggga atttcagcat gggaatgctg gtgtttatct tcgtcgctcg
    18781 ggtcattacc accttactct gcttctcttc cggcgcgccg ggcggtattt ttgccccgat
    18841 gctggcgctg ggtactgtgc tgggaaccgc tttcggaatg gttgccgttg agctgtttcc
    18901 gcaatatcac ctggaggcgg ggacgtttgc tattgccgga atgggggcat tactggcggc
    18961 atctattcgc gcgccgttaa cggggatcat tctggttctg gagatgaccg ataactacca
    19021 gctcattttg ccaatgatta ttaccggtct tggcgcaaca ctattagcgc aatttaccgg
    19081 cgggaaaccg ctatactcgg cgattcttgc gcgcacgctg gcaaaacagg aagctgagca
    19141 actggcgcga agcaaggccg catcagccag cgagaatact tgaacgaaat accagggtat
    19201 tagataatgg cgattattat tgggttagaa tttgcccaat tgccgatgtc gtttggagca
    19261 aaatatgagt gatgacgtag cactgccgct ggagtttacc gacgcagcag ccaacaaagt
    19321 taaaagcctg atcgctgacg aagataaccc gaatctgaaa ttacgcgtgt atatcaccgg
    19381 tggcggttgc agcggcttcc agtatggttt cacctttgat gatcaggtga acgaaggcga
    19441 tatgaccatc gaaaaacagg gcgttggcct ggtggttgat ccgatgagcc tgcaatatct
    19501 ggtcggcggt tccgttgact ataccgaagg actggaaggt tctcgtttca tcgtgaccaa
    19561 cccgaacgcg aaaagcacct gcggttgcgg ttcttccttt agcatctaat ctgttgttca
    19621 tgtgccggat aaactatccg gcacatcttc tctcttcaac gcccattctc atccagcgca
    19681 aacgtcggta gcttaagatg ccaacgaata gccgccagcc gaatcaatag cgtcacgacc
    19741 atgcccatca tactggctgt ttccagtggt acggaaaatg tgtaataagc cgtagcgtgg
    19801 acaataccgc cgataataca ggcagttgcg tagatttctg tacgtaaaat catggggatc
    19861 tcgcgggcca gtacatcacg aataatcccg ccgccaacgc cagtaatgac gcccatgcaa
    19921 accgcgatta acggaccggc ttccgcatta aaggctttat tcacgccaat gccgacaaac
    19981 accgccagac caacggcgtc cagcaccggc aacatccatt ttggtaagcg tcttggctgg
    20041 cgcaccagca cgatggtcag catgctggtg accattgcaa cgaccagatc ggtgggatct
    20101 ttcacccaaa ataccgggcc gtgatccagc gccatgtcgc gaattgttcc gccgcctact
    20161 gcggttacca cgcccagtac cagaacacca aaagggtcca tacgcaattt tccggctaac
    20221 aaaacgccgg agatggcaaa taccgctgtg ccgactatat ccagccaata gacgagcatt
    20281 gttcaatccc cactgagcac ctgaaaaggt caggcactaa tctacctgtg aaagcgcatt
    20341 acagagctgt tgtgctgcga ggataatacg tgggcttgca cgttcaaacc agtcactcgt
    20401 gagaggaata acgggaattt tgagctgttc accccagtat tgtttgattt taggaatttg
    20461 gtccggtccg cctgtaatga caatcgcctg tggcgagcgt gctaacacct gttcgcggct
    20521 aacttgcggc cagggaaccc ggctgtcttt aaagatgttt tctccgccac aaacttcgag
    20581 tacctggttc tgaatcgact cttttccact ggtaaatggc ggattaatgc cgaattgcag
    20641 aaaaacacgt tttttaggtt tatcagcata ttgcgctttc aattgcgcgt actgatccag
    20701 cagggattgc gcggcttgtt cggccttgtc tggttgcgga ctccaggggg ccagttgacg
    20761 taacgcattg gcaatttgtt caatgctggt cgcatcgacc cacatcactt ttattcccag
    20821 cgaagccagc tggtcaacct gccgctcggc attacctcca cgccaggcaa tcaccagatc
    20881 gggtttcagc gcgacaatgc gttccagatt catcccctgc caggtggaaa cctgctcaat
    20941 cttttgcgct tgtggaggat agtcggaata gctgctgacc ccaaccggcg tgatcccggc
    21001 ggcaaaggca agttcagtgt tggcgggaga aagcgtgatg acgcgcggcg cggcgttgag
    21061 ccacagtggc gcaagaaaag acagggcgac cagcgccctg aacagtgact tagccatgtg
    21121 caagtttctg caccagtgac tcaaccatca ggctggactg tttagcggca acagccagga
    21181 actcatcgaa gctaagatga gactgttgat cggccacgtc ggagatggcg cgtacgacaa
    21241 caaacgggac gttgaaattg tggcagacat gggcgattgc cgtcgcttcc atctctacag
    21301 caatggcctg tgggaagttg tggcggattt tcgccagacc aacagaaccg ttgatgaaag
    21361 cgtcgccgct aacaatcagg ccacgtacag cgttaagatt cagttcggca atgcaggcct
    21421 cagcggcagc gatcagttta tcgtcagctt taaagcctgc cggacagcct ggtaactgac
    21481 cgtattcata accaaatgcc gtgacatccg cgtcgtgata acgtgcttcg tccgagacaa
    21541 cgatatcgcc cactttcaac gttggtgcca ggccaccggc agaaccggtg ttaataatca
    21601 catctggctt gcagtgttcc aacagcaaag tggcacccag cgccgcagcg actttaccga
    21661 tgcccgattt cagaagcgca acctcggttc cattcagttg gccggtatag atttcgcaac
    21721 cgccgagact gatagtttga cggttttcga ttttgtcacg cagcagcgta acttcttctt
    21781 ccattgcacc aatgatgccg attttcatag atttactcgc gataagcccg atttgaaggc
    21841 atagtttacc atgcgcttac ggggaagcgt atttctcacg cgggagagga catggcacag
    21901 attgatttcc gaaaaaaaat aaactggcat cgtcgttacc gttcaccgca gggcgttaaa
    21961 accgaacatg agatcctgcg gatcttcgag agcgatcgcg ggcgtatcat caactctccg
    22021 gcaattcgtc gtctgcaaca aaagacccag gtttttccac tggagcgcaa tgccgccgtg
    22081 cgcacgcgtc ttacccactc gatggaagtc cagcaggtgg ggcgctacat cgccaaagaa
    22141 attctaagcc gtctgaaaga gcttaaatta ctggaagcat acggcctgga tgaactgacc
    22201 ggcccctttg aaagcattgt tgagatgtca tgcctgatgc acgatatcgg caatccgccg
    22261 tttggtcatt ttggcgaagc ggcgataaat gattggtttc gccagcgttt gcacccggaa
    22321 gatgccgaaa gccagcctct gactgacgat cgctgcagcg tggcggcact acgtttacgg
    22381 gacggggaag aaccgcttaa cgagctgcgg cgcaagattc gtcaggactt atgtcatttt
    22441 gaggggaatg cacaaggcat tcgtctggtg catacattga tgaggatgaa tctcacctgg
    22501 gcacaggttg gcggtatttt aaaatatacc cgtccggcgt ggtggcgtgg cgaaacgcct
    22561 gagacacatc actatttaat gaaaaagccg ggttattatc tttctgaaga agcctatatt
    22621 gcccggttgc gtaaagaact taatttggcg ctttacagtc gttttccatt aacgtggatt
    22681 atggaagctg ccgacgacat ctcctattgt gtggcagacc ttgaagatgc ggtagagaaa
    22741 agaatattta ccgttgagca gctttatcat catttgcacg aggcgtgggg tcagcatgag
    22801 aaaggttcgc tcttttcgct agtggttgaa aatgcctggg aaaaatcacg ctcaaatagt
    22861 ttaagccgca gtacggaaga tcagtttttt atgtatttac gggtaaacac cctaaataaa
    22921 ctggtaccat acgcggcaca acgatttatt gataatctgc ctgcgatttt cgccggaacg
    22981 tttaatcatg cattattgga agatgccagc gaatgcagcg atcttcttaa gctatataaa
    23041 aatgtcgctg taaaacatgt gtttagccat ccagatgtcg agcagcttga attgcagggc
    23101 tatcgggtca ttagcggatt attagagatt tatcgtcctt tattaagcct gtcgttatca
    23161 gactttactg aactggtaga aaaagaacgg gtgaaacgtt tccctattga atcgcgctta
    23221 ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg aggctgtcag taaattaccg
    23281 tcagattctc ctgagtttcc gctatgggaa tattattacc gttgccgcct gttgcaggat
    23341 catatcagcg gtatgaccga cctctatgcg tgggatgaat accgacgtct gatggccgta
    23401 gaacaataac caggcttttg taaagacgaa caataaattt ttaccttttg cagaaacttt
    23461 agttcggaac ttcgcgctat aaaacgaatc tgaagaacac agcaattttg cgttatctgt
    23521 taatcgagac tgaaatacat gaaaaaaacc acattagcac tgagtgcact ggctctgagt
    23581 ttaggtttgg cgttatctcc gctctctgca acggcggctg agacttcttc agcaacgaca
    23641 gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg tgatgccttc agtggtcagc
    23701 attaacgtag aaggtagcac aaccgttaat acgccgcgta tgccgcgtaa tttccagcag
    23761 ttcttcggtg atgattctcc gttctgccag gaaggttcac cgttccagag ctctccgttc
    23821 tgccaggggg gccagggcgg taatggcggc ggccagcaac agaaattcat ggcgctgggt
    23881 tccggcgtca tcattgatgc cgataaaggc tatgtcgtca ccaacaacca cgttgttgat
    23941 aacgcgacgg tgattaaagt ccagctgagc gatggtcgta agttcgacgc gaagatggtt
    24001 ggcaaagatc cgcgctctga tatcgcgctg attcagatcc agaacccgaa aaacctgacc
    24061 gcaattaaga tggcggattc tgatgcactg cgcgtgggtg attacaccgt agcgattggt
    24121 aacccgtttg gcctgggaga gacggtaact tccgggattg tctctgcgct ggggcgtagc
    24181 ggcctgaatg ccgaaaacta cgaaaacttc atccagaccg atgcagcgat caaccgtggt
    24241 aactccggtg gtgcgctggt taacctgaac ggcgaactga tcggtatcaa caccgcgatc
    24301 ctcgcaccgg acggcggcaa catcggtatc ggttttgcta tcccgagcaa catggtgaaa
    24361 aacctgacct cgcagatggt ggaatacggc caggtgaaac gcggtgagct gggtattatg
    24421 gggactgagc tgaattccga actggcgaaa gcgatgaaag ttgacgccca gcgcggtgct
    24481 ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag cgggcattaa agcgggtgat
    24541 gtgatcacct cactgaacgg taagccaatc agcagctttg ccgcactgcg tgctcaggtg
    24601 ggtactatgc cggtgggcag caaactgacc ctgggcttgc tgcgcgacgg taagcaggtc
    24661 aacgtgaatc tggaactgca gcagagcagc cagaatcagg ttgattccag ctccatcttc
    24721 aacggcattg aaggcgctga gatgagcaac aaaggcaaag atcagggcgt ggtagtgaac
    24781 aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga agaaaggtga tgtgattatt
    24841 ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc gtaaagttct cgacagcaaa
    24901 ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca ccatctacct gttaatgcag
    24961 taatctccct caaccccttc ctgaatacgg gaaggggttc tccttacaat ctgtgaactt
    25021 cgccacaact ccatacatct tcatcatcct ttgggcattt gcacaatgcc gtacgttacg
    25081 tacttcctta tgctaagccg tgcataacgg aggacttatg gctggctggc atcttgatac
    25141 caaaatggcg caggatatcg tggcacgtac catgcgcatc atcgatacca atatcaacgt
    25201 aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt gagcgtattg gtgaattgca
    25261 cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc gatatcgatg acgcggtggc
    25321 acgccatctg cacggtgtgc ggcaggggat taatctaccg ttacggctgg aaggtgaaat
    25381 tgtcggcgta attggcctga caggtgaacc agagaatctg cgtaaatatg gcgaactggt
    25441 ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg atgcacctgt tggcgcagga
    25501 tagccgtttg cgggaagaac tggtgatgaa cctgattcag gctgaggaga atactcccgc
    25561 acttactgaa tgggcgcaac ggctggggat cgatctcaat caaccgcgcg tagtggctat
    25621 tgttgaggtc gacagcggtc agcttggcgt ggacagcgca atggcggagt tacaacaact
    25681 gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg gcgattgtct cgctgaccga
    25741 aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc tgggatgcag aagatcatcg
    25801 taagcgagtt gaacaactga ttacccgcat gaaagagtat ggccagctgc gttttcgcgt
    25861 ttcgctgggc aactatttta ccggtcctgg cagtattgcc cgatcctatc gtacggcgaa
    25921 aacgacgatg gtggtgggta aacagcggat gccagaaagt cgctgctatt tttatcagga
    25981 tctgatgtta cctgtgttac tcgacagttt gcgtggcgac tggcaggcca atgaactggc
    26041 gcgaccgctg gcgcggctga aagcgatgga caataatggc ttgctgcgac gaacgctggc
    26101 ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca aaggcgctgt ttattcatcg
    26161 taatactctg gagtatcggc ttaatcgtat atcggaactg accgggcttg atttgggtaa
    26221 ttttgatgac aggttgctgc tgtatgtggc gttgcagctg gatgaagagc ggtaggttat
    26281 gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc cgaagggtag aggggaatgt
    26341 ccgagttaac cgtcagttcg gcattattta cgcgttagtt tttccagatc ggcttcaatc
    26401 tcgctgatct tattggttac gactgactcc aggtgacgta agtcgtcgag gatcttacgc
    26461 ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat caagctcatc aatgatatac
    26521 cgtagattcg ggctgatttc ctggacttct ttataaccct gacccacacc atcagcgacg
    26581 accgttttac gctgacgcgg atatttaaac ttaacgctct tggcgaaaaa ctcgcctttg
    26641 tctttctgga aatagatttt cagaatatcg ttgttggctt cctgccggag gctgtaacga
    26701 tcaatttctt caggattggt aatacccaga cttttcagat tgtcgtacat agcgttacct
    26761 caaaatgagt cagtaaattg tgcttatttt agcatttggc ctggcccgcc cggctcgact
    26821 gtgatcgcag aaagcctttt tcttccagac ttctatcatg gcgtaaaaat caaaaaatta
    26881 cctgctttat tgtggcgata ttccatggga tggattaaca catttatctt cgttttgcga
    26941 attacatgac agtccgaaac aagatgaaaa agtatttaaa aaagggggga agaagaatga
    27001 gtaggacgaa ttattctgag ccagaaagca tacgggcagc atcatgctgc ccgtatatgt
    27061 actcttaatc gatggtacgc agcagttcgt taatgccgac tttgccgcga gttttcgcgt
    27121 caactttctt aacgataacc gcacagtaga ggctgtattt gccatctttc gacggcagat
    27181 tacctgaaac aaccacagac cccgccggaa cgcgaccgta gtggatttcg ccggtttcac
    27241 ggtcgtaaat acgggtgctc tgaccaatgt atacgcccat ggaaatgacg gaaccttctt
    27301 cgacaatcac cccttcaacc acttcagagc gtgcgccaat gaagcagtta tcttcaatga
    27361 tggttgggtt agcctgcagc ggttccagca cgccgccgat gcccacgcca ccggaaaggt
    27421 ggacgttttt accaatctgc gcacaagaac cgacggtcgc ccaggtatca accatggtgc
    27481 cttcatcaac atatgcgccg atgttgacgt aagacggcat cagcacggtg ttacgggcaa
    27541 taaacgcacc ctgacgtacc gccgctggtg gcacaacgcg gaagccttct ttctggaaac
    27601 gtgcttcgtc gtagtcggcg aatttcatcg gcactttgtc gaagtagcgg ctttctgccc
    27661 cttcgatcac ctgattatca ttaatacgga aagagagcag caccgctttt ttcaaccact
    27721 gatgcgtcac ccactgaccg tcaatttttt ccgctacacg cagtgcaccg gaatccagca
    27781 gggcgatcac ctgatttacc gcttcgcggg taacggtgtc tgcgttggct ggcgtgatct
    27841 cggcacggcg ttcaaaagcg gtttcaataa tgttctgtaa ctgctgcatt gttaaactct
    27901 tttcatatca gtaaacacat caccctttat cgtttggatt gagggcctct gtcaaccgct
    27961 gatgcacttc ctgctgcaac tcgttattaa gtgcacgccg gtcagcggtg gcaattatga
    28021 ataaatcttc tactcgctcg ccaatggttg taattcgggc accatgaagc gaaattccca
    28081 gatcggcaaa aattttcccg actctcgcca gcagtcctgg ctggtcgagc gcgataagct
    28141 caaggaacga tttgcggtcg gtatgggtcg gcaaaaacgt tacttcggtt tcaacagtaa
    28201 aatggcgtaa tttggcgggt tggcgacggg gctgcggtgg ctgccagcta ctttgcgtca
    28261 gtacttgctc cagaccaaac cgaataacct catgacgatc tgcggacagc gggctgccat
    28321 cgggttccag cacgataaag gtatccatcg ccataccgtc gcgagtggtg aaaatttgtg
    28381 cgtcgtgaac acttaaattg cggcggtcta attcggcaca gacggcggca aacagatagg
    28441 ggcggtccgg gctccagata aaaatctcgg tgcctccacg cgtagcctgc gggctaagca
    28501 ataccagcgg tttgcttaaa tcatgctgta ataaatggcg ggcatgccag gccagttgat
    28561 ttgggctatg gcggacaaaa tagttagcac gacagcgtga ccaaatttgg tgcagcgcct
    28621 cttcgtcgat gttatccatg cgcagtagtg ccagtgcctg gagttggtga tggcgaaccc
    28681 gttcgcgcat atccggcgtg ttttgcattc cgcgtcgtag ctgcttttcg gtggcaaaat
    28741 agagttcgcg caacagactt tgcttccagc tattccacag cgtttcgttg gtggcgcaaa
    28801 tgtcagccac tgtcaggcat accagatagc gcagacgatt ttccgtttga acttcttcgg
    28861 caaactgctt gatgacttca gggtcctgaa tatcgcggcg ttgtgctgtc accgacatca
    28921 gcaagtgctg gcgaaccagc caggcaacca gctgtgtttc gcgtgagttc agcccgtgga
    28981 gttctgcaaa atgcactaca tcctgagcac cgagaatgga gtggtcgccg ccgcgtcctt
    29041 tggcgatatc gtgaaacagc gcggcgatga aaatcagctc agttgacggc aggcgcggcc
    29101 agacgtccac acacaacgga tggcgctggc gcgtttcttc actggcaaaa ctctccagtt
    29161 tcagcatcac gcggatagta tgttcatcca ccgtgtaggc gtggaacaga tcaaactgca
    29221 tctgcccgac gatatgcgac cattgcggca tatacgcgcc gagcacgcta tggcgatgca
    29281 ttggcaatag cccgcgccgc accgctccgg ggtgacgcag aatgctcaaa aacagttttc
    29341 gtgcttccgg aatattacac agcggttgtt gcagatggcg acgggcatgg cgtaactggc
    29401 gcagcgtggt ggagtaaatg ccggtgatcg cactgttgcg caccatggtg tagaacatac
    29461 gcaagatggc ttccggctgg cgcataaata gtgtttcatc acgcaggtcg attagcgtac
    29521 cgcgtagctg aaactcatcg tcgattggac gtggtttttc gtcggcggga agggcgagga
    29581 tggcttcatc gaacagttgc agcagcatct ggttgagttc actgacgcgg cgtgtaacgc
    29641 ggaagtaatc cttcatcatc cgctcgaccg gttcgttgcc ttcaccactg taattcagac
    29701 gctgagcgac gctaagttgg cgatcgaata acaggcgatt atcgtaacgg ctgacgacca
    29761 gatgcagggc aaagcgaata cgccacagta tatgcagaca ttcgtttaat tccgcccgct
    29821 ccgctgaggt taagaagcca aacccaacca tttcatccag cgatgttgcg ccaaaatgac
    29881 ggcgggccac ccattgcaga gtgtggatat cgcgcaagcc gccagggcta cttttgatgt
    29941 ctggttcaag attgtagctg gtgccgtggt aacgctgatg gcgctggttc tgttcttcaa
    30001 ctttcgccgc gtagaacttg tcggaaggcc agaatccttc gctaaaaata tgtttttgca
    30061 gttcgaggaa gagcgcaaca tcgccaatta ataagcggga ttcgattaaa ttggtggcga
    30121 cggttaaatc cgataaccct tccagcatgc actcttcaag cgtgcgcaca ctatgaccga
    30181 cttccagctt tacatcccag agcagcgtta acagctcgcc cactttttgc gcctgatcgt
    30241 ccgggagctt tttacggctt aaaatcagta aatcgatgtc agaaagtgga tgcagctcgc
    30301 cacgaccgta gccaccgacg gcgaccaatg ccaggtcggc aatctggctg aatcccgctt
    30361 caatccataa tcgttgcagg agctggtcga taaactcggt gcgcgcctca atcaactgtt
    30421 ctgcagagat cccattgtca aaggcatcac ccagccaacg ctggaaagta tcgatatggg
    30481 cttttatccc accgacggtt aattcatcac ggggccagac gcatggattt tgcggttgac
    30541 cgggcagggt ggggagagcg gtgtttgcgt actgttctgg aagggtattc attgtgcgcc
    30601 acccataaga ttaaattatc gcattaaaaa agccggcatt cgccggcttc atcttattcg
    30661 tcgtgcgaga ttatcgccgg gatggtgtca tccttgcgta gcgtcagaat ttcgcagccg
    30721 ttatcagtca ccacaatagt atgctcatat tgtgcagaca agctgcgatc tttggttttt
    30781 accgtccagc catctttcat ggtgcggatc tcttttttac ccgcgttgac cattggctcg
    30841 atggtgaacg tcatcccagg tttcagtacg acgttggttt cacgggagtc atagtgcagc
    30901 acctgcggtt cttcatggaa gccgcgacca ataccgtgtc cgcaatattc acgaacgacg
    30961 gagaagcctt ctgcttcgac aaacttctga atcgccgcac cgatttcgcg caggttaatg
    31021 cctggtttga ccatgcgcag cgccaggtac aggctttctt gcgtgatgcg gcacagacgt
    31081 tcgcccatga tggtcggctt accgacgata aacattttcg aggtatcgcc gtggaaacca
    31141 tctttgatta cggtgacatc aatgttaacg atatcgccat ctttcagcag cttcgcatcg
    31201 tccgggatac cgtggcacac cacttcatta atagagatgc aaacggattt cggatagccg
    31261 tgatagccga ggcaggcaga aaccgcgtgt tgttcattaa caatgtaatc attacagatg
    31321 cgatccagct cgccggtgct gacgcccggt ttaacatacg gttcgatcat ctccagcact
    31381 tcggcagcca gtcggccagc gacgcgcatt ttttcgatat cttctggggt cttgattgag
    31441 atagccatta attctgtcca tcagcgtcgg tgataccgac aatatatatg taagtgccgt
    31501 caatggtatc acacccgggc aaattgagaa tcattctgaa tttcgccaaa cgtgccactg
    31561 aacgttttct ataatagaaa attcaacgtc tgatgctgta cacagcgcca acaattattg
    31621 gtgtccatga cgtatttgtg gtataaagcg cgccggactt ccgatccatt tcgtatacac
    31681 agactggacg gaagcgacaa tctcactttg tgtaacaaca cacacgtatc ggcacatatt
    31741 ccggggtgcc ctttggggtc ggtaatatgg gatacgtgga ggcataaccc caacttttat
    31801 atagaggttt taatcatggc aactgtttcc atgcgcgaca tgctcaaggc tggtgttcac
    31861 ttcggtcacc agacccgtta ctggaacccg aaaatgaagc cgttcatctt cggtgcgcgt
    31921 aacaaagttc acatcatcaa ccttgagaaa actgtaccga tgttcaacga agctctggct
    31981 gaactgaaca agattgcttc tcgcaaaggt aaaatccttt tcgttggtac taaacgcgct
    32041 gcaagcgaag cggtgaaaga cgctgctctg agctgcgacc agttcttcgt gaaccatcgc
    32101 tggctgggcg gtatgctgac taactggaaa accgttcgtc agtccatcaa acgtctgaaa
    32161 gacctggaaa ctcagtctca ggacggtact ttcgaaaagc tgaccaagaa agaagcgctg
    32221 atgcgcactc gtgagctgga gaaactggaa aacagcctgg gcggtatcaa agacatgggc
    32281 ggtctgccgg acgctctgtt tgtaatcgat gctgaccacg aacacattgc tatcaaagaa
    32341 gcaaacaacc tgggtattcc ggtatttgct atcgttgata ccaactctga tccggacggt
    32401 gttgacttcg ttatcccggg taacgacgac gcaatccgtg ctgtgaccct gtacctgggc
    32461 gctgttgctg caaccgtacg tgaaggccgt tctcaggatc tggcttccca ggcggaagaa
    32521 agcttcgtag aagctgagta ataaggcttg ataactcccc caaaatagtt cgagttgcag
    32581 aaaggcggca agctcgagaa ttcccgggag cttacatcag taagtgaccg ggatgagcga
    32641 gcgaagataa cgcatctgcg gcgcgaaata tgaaggggga gagcccttat agaccaggta
    32701 gtacacgttt ggttaggggg cctgcatatg gccccctttt tcacttttat atctgtgcgg
    32761 tttaatgccg ggcagatcac atctccgagg attttagaat ggctgaaatt accgcatccc
    32821 tggtaaaaga gctgcgtgag cgtactggcg caggcatgat ggattgcaaa aaagcactga
    32881 ctgaagctaa cggcgacatc gagctggcaa tcgaaaacat gcgtaagtcc ggtgctatta
    32941 aagcagcgaa aaaagcaggc aacgttgctg ctgacggcgt gatcaaaacc aaaatcgacg
    33001 gcaactacgg catcattctg gaagttaact gccagactga cttcgttgca aaagacgctg
    33061 gtttccaggc gttcgcagac aaagttctgg acgcagctgt tgctggcaaa atcactgacg
    33121 ttgaagttct gaaagcacag ttcgaagaag aacgtgttgc gctggtagcg aaaattggtg
    33181 aaaacatcaa cattcgccgc gttgctgcgc tggaaggcga cgttctgggt tcttatcagc
    33241 acggtgcgcg tatcggcgtt ctggttgctg ctaaaggcgc tgacgaagag ctggttaaac
    33301 acatcgctat gcacgttgct gcaagcaagc cagaattcat caaaccagaa gacgtatccg
    33361 ctgaagtggt agaaaaagaa taccaggtac agctggatat cgcgatgcag tctggtaagc
    33421 cgaaagaaat cgcagagaaa atggttgaag gccgcatgaa gaaattcacc ggcgaagttt
    33481 ctctgaccgg tcagccgttc gttatggaac caagcaaaac tgttggtcag ctgctgaaag
    33541 agcataacgc tgaagtgact ggcttcatcc gcttcgaagt gggtgaaggc atcgagaaag
    33601 ttgagactga ctttgcagca gaagttgctg cgatgtccaa gcagtcttaa ttatcaaaaa
    33661 ggagccgcct gagggcggct tctttttgtg cccatcttgt aaattcagct aacccttgtg
    33721 gggctgcgct gaaaagcgac gtacaatgtc gctagtatta attcatttca atcgttgaca
    33781 gtctcaggaa agaaacatgg ctaccaatgc aaaacccgtc tataaacgca ttctgcttaa
    33841 gttgagtggc gaagctctgc agggcactga aggcttcggt attgatgcaa gcatactgga
    33901 tcgtatggct caggaaatca aagaactggt tgaactgggt attcaggttg gtgtggtgat
    33961 tggtgggggt aacctgttcc gtggcgctgg tctggcgaaa gcgggtatga accgcgttgt
    34021 gggcgaccac atggggatgc tggcgaccgt aatgaacggc ctggcaatgc gtgatgcact
    34081 gcaccgcgcc tatgtgaacg ctcgtctgat gtccgctatt ccattgaatg gcgtgtgcga
    34141 cagctacagc tgggcagaag ctatcagcct gttgcgcaac aaccgtgtgg tgatcctctc
    34201 cgccggtaca ggtaacccgt tctttaccac cgactcagca gcttgcctgc gtggtatcga
    34261 aattgaagcc gatgtggtgc tgaaagcaac caaagttgac ggcgtgttta ccgctgatcc
    34321 ggcgaaagat ccaaccgcaa ccatgtacga gcaactgact tacagcgaag tgctggaaaa
    34381 agagctgaaa gtcatggacc tggcggcctt cacgctggct cgtgaccata aattaccgat
    34441 tcgtgttttc aatatgaaca aaccgggtgc gctgcgccgt gtggtaatgg gtgaaaaaga
    34501 agggacttta atcacggaat aattcccgtg atggataaat aagggtaaga ttccgcgtaa
    34561 gtatcgcggg ggcgtaagtc tggttataag gcgttattgt tgcaggcagt ttggtcacgg
    34621 ccagcgcgca gcaaccggag cgtacaaaag tacgtgagga tggcgagcac tgctcggggc
    34681 caaaatggca aataaaatag cctaataatc cagacgatta cccgtaatat gtttaatcag
    34741 ggctatactt agcacacttc cactgtgtgt gactgtctgg tctgactgag acaagttttc
    34801 aaggattcgt aacgtgatta gcgatatcag aaaagatgct gaagtacgca tggacaaatg
    34861 cgtagaagcg ttcaaaaccc aaatcagcaa aatacgcacg ggtcgtgctt ctcccagcct
    34921 gctggatggc gttgtcgtgg aatattacgg cacgccgacg ccgctgcgtc agctggcaag
    34981 cgtaacggta gaagattccc gtactctgaa aatcaacgtg tttgatcgtt caatgtctcc
    35041 ggccgttgaa aaagcgatta tggcgtccga tcttggcctg aacccgaact ctgcgggtag
    35101 cgacatccgt gttccgctgc cgccgctgac ggaagaacgt cgtaaagatc tgaccaaaat
    35161 cgttcgtggt gaagcagaac aagcgcgtgt tgcagtacgt aacgtgcgtc gtgacgcgaa
    35221 cgacaaagtg aaagcactgt tgaaagataa agagatcagc gaagacgacg atcgccgttc
    35281 tcaggacgat gtacagaaac tgactgatgc tgcaatcaag aaaattgaag cggcgctggc
    35341 agacaaagaa gcagaactga tgcagttctg atttcttgaa cgacaaaaac gccgctcagt
    35401 agatccttgc ggatcggctg gcggcgtttt gctttttatt ctgtctcaac tctggatgtt
    35461 tcatgaagca actcaccatt ctgggctcga ccggctcgat tggttgcagc acgctggacg
    35521 tggtgcgcca taatcccgaa cacttccgcg tagttgcgct ggtggcaggc aaaaatgtca
    35581 ctcacatggt agaacagtgc ctggaattct ctccccgcta tgccgtaatg gacgatgaag
    35641 cgagtgcgaa acttcttaaa acgatgctac agcaacaggg tagccgcacc gaagtcttaa
    35701 gtgggcaaca agccgcttgc gatatggcag cgcttgagga tgttgatcag gtgatggcag
    35761 ccattgttgg cgctgctggg ctgttaccta cgcttgctgc gatccgcgcg ggtaaaacca
    35821 ttttgctggc caataaagaa tcactggtta cctgcggacg tctgtttatg gacgccgtaa
    35881 agcagagcaa agcgcaattg ttaccggtcg atagcgaaca taacgccatt tttcagagtt
    35941 taccgcaacc tatccagcat aatctgggat acgctgacct tgagcaaaat ggcgtggtgt
    36001 ccattttact taccgggtct ggtggccctt tccgtgagac gccattgcgc gatttggcaa
    36061 caatgacgcc cgatcaagcc tgccgtcatc cgaactggtc gatgggacgt aaaatttctg
    36121 tcgattcggc taccatgatg aacaaaggtc tggaatacat tgaagcgcgt tggctgttta
    36181 atgccagcgc cagccagatg gaagtgctga ttcacccgca gtcagtgatt cactcaatgg
    36241 tgcgctatca ggacggcagt gttctggcgc agctggggga accggatatg cgtacgccaa
    36301 ttgcccacac catggcatgg ccgaatcgcg tgaactctgg cgtgaagccg ctcgattttt
    36361 gcaaactaag tgcgttgaca tttgccgcac cggattatga tcgttatcca tgcctgaaac
    36421 tggcgatgga ggcgttcgaa caaggtcagg cagcgacgac agcattgaat gccgcaaacg
    36481 aaatcaccgt tgctgctttt cttgcgcaac aaatccgctt tacggatatc gctgcgttga
    36541 atttatccgt actggaaaaa atggatatgc gcgaaccaca atgtgtggac gatgtgttat
    36601 ctgttgatgc gaacgcgcgt gaagtcgcca gaaaagaggt gatgcgtctc gcaagctgag
    36661 gataatccgg ctacagagag tcgcgctatt tgttagcgta gggcttcagt gatatagtct
    36721 gcgccatctg atcgtaagta gttggcttta taaggtcagg tatgccgtgg ttttacacgg
    36781 cttttttttg tataggcttc agtattcctg agtaccgtaa accctgtcag ggaataaaaa
    36841 acgcgtgatg ttgtctgcta ctcaaccact tagcgaaaaa ttgccagcgc atggctgccg
    36901 tcatgttgcg atcattatgg acggcaatgg tcgctgggca aaaaagcaag ggaagattcg
    36961 tgcctttggg cataaagccg gggcaaaatc cgtccgccgg gctgtctctt ttgcggccaa
    37021 caacggtatt gaggcgttaa cgctgtatgc ctttagtagt gaaaactgga accgaccagc
    37081 gcaggaagtc agtgcgttaa tggaactgtt tgtgtgggcg ctcgatagcg aagtaaaaag
    37141 tctgcaccga cataacgtgc gtctgcgtat tattggcgat accagtcgct ttaactcgcg
    37201 tttgcaagaa tgtattcgta aatctgaagc gctaacagcc gggaataccg gtctgacgct
    37261 gaatattgcg gcgaactacg gtggacgttg ggatatagtc cagggagtca ggcaactggc
    37321 tgaaaaggtg cagcaaggaa acctgcaacc agatcagata gatgaagaga tgctaaacca
    37381 gcatgtctgt atgcatgaac tggcccctgt agatttagta attaggactg ggggggagca
    37441 tcgcattagt aactttttgc tttggcaaat tgcctatgcc gaactttact ttacagatgt
    37501 tctctggccc gatttcgatg aacaagactt tgaaggggcg ttaaatgcct ttgctaatcg
    37561 agagcgtcgt ttcggcggca ccgagcccgg tgatgaaaca gcctgatggg ggtcgctttt
    37621 gctgaagtat cgcctgatat ctgcttttgt gttaataccc gtcgtcatcg cggcgttgtt
    37681 tctgttgccg ccggtggggt tcgccatcgt aacgctggtg gtctgcatgc tggcagcgtg
    37741 ggaatgggga cagcttagcg gttttaccac tcgtttgcag cgagtatggt tggcggtgtt
    37801 atgcgggtta ttgttggcgc tgatgctttt tctgttgccg gaatatcacc gaaatattca
    37861 tcaaccgctg gttgaaatct cactttgggc ttcgctgggt tggtggattg tcgcgctatt
    37921 gctggtgctg ttttacccag gttccgcagc aatctggcgt aactctaaaa cattgcgcct
    37981 tatttttggc gtgctaacca ttgttccctt cttctggggc atgctggcgt tacgggcctg
    38041 gcactacgac gagaatcatt acagtggcgc aatatggctg ctctatgtca tgatcctggt
    38101 atggggcgct gactctggcg catatatgtt tggcaaattg tttggtaaac ataagctggc
    38161 accgaaggtt tctccgggta aaacctggca aggctttata ggtggactag cgaccgcagc
    38221 ggtaatctca tggggttatg gcatgtgggc gaatctcgac gtcgctcccg tcaccttact
    38281 catttgctct attgtcgcag cgttagcctc agtgctcggc gatctgaccg agagtatgtt
    38341 taagcgtgaa gcaggaatta aggacagcgg tcatttaatt ccaggacacg gtggtatttt
    38401 agatcgtatt gatagcctga cggctgcggt accggtcttt gcttgcttgt tgttactggt
    38461 attcaggacg ctttaacgga aggtaatatg ctgagttttc tctgggattt ggcttcgttc
    38521 atcgttgcac tgggtgtact tatcaccgtg catgaatttg gtcatttctg ggttgcccgg
    38581 cgttgtggtg ttcgcgttga gcgtttctca atagggtttg gtaaggcgct ctggcggcga
    38641 actgataagc tcggcaccga atatgttatc gccctgatcc cgctgggcgg ttatgtcaaa
    38701 atgctggatg agcgcgcaga accggtcgtt ccggaactcc gccaccatgc cttcaataat
    38761 aaatctgtcg gccaacgagc ggcgattatt gccgcaggtc cggttgcaaa cttcattttt
    38821 gctatctttg cctactggct ggtttttatt attggtgtgc ctggcgtacg tccggtggtt
    38881 ggtgaaatag cagccaattc gatagctgcg gaagcacaaa ttgcaccagg tacggaacta
    38941 aaagccgtag atggtatcga aacgcctgat tgggatgccg tgcgtttgca gttggtcgat
    39001 aaaattggcg atgaaagcac caccattaca gtagcgccat ttggcagcga ccaacggcgg
    39061 gatgtaaagc tcgatttacg tcactgggcg tttgagcctg ataaagaaga tccggtatct
    39121 tcgctgggga ttcgtcctcg tgggccgcaa attgaacctg tactggaaaa tgtgcaacca
    39181 aactcggcgg caagcaaggc aggtttgcaa gcaggcgaca ggatcgttaa agtcgatggt
    39241 cagcccttaa cgcagtgggt gacctttgtg atgcttgtcc gggataaccc gggtaaatcc
    39301 ttagcgttag aaatcgaaag gcagggaagt cccttgtctt tgacattaat cccggagagt
    39361 aaaccgggta atggtaaagc gattggtttt gtcggtattg agccgaaagt cattcctttg
    39421 ccagatgagt ataaagttgt acgccagtat gggccgttca acgccatcgt cgaagccacg
    39481 gacaaaacgt ggcagctgat gaagctgacg gtcagtatgc tgggaaaatt gatcaccggt
    39541 gatgtgaaac tgaacaacct cagtgggccg atctctatcg ccaagggggc tgggatgaca
    39601 gcggaactcg gggtagttta ttacctgccg tttcttgcgc ttattagcgt gaacttaggg
    39661 ataattaacc tgtttccgtt gcccgtactt gacggggggc atctgctgtt ccttgcgatc
    39721 gaaaagatca agggcggacc ggtatccgag cgggttcaag acttttgtta tcgcattggc
    39781 tcgattctgc tggtgctgtt aatggggctt gcacttttca atgatttctc tcggttatga
    39841 gagttagtta ggaagaacgc ataataacga tggcgatgaa aaagttgctc atagcgtcgc
    39901 tgctgtttag cagcgccacc gtatacggtg ctgaagggtt cgtagtgaaa gatattcatt
    39961 tcgaaggcct tcagcgtgtc gccgttggtg cggccctcct cagtatgccg gtgcgcacag
    40021 gcgacacggt taatgatgaa gatatcagta ataccattcg cgctctgttt gctaccggca
    40081 actttgagga tgttcgcgtc cttcgtgatg gtgataccct tctggttcag gtaaaagaac
    40141 gtccgaccat tgccagcatt actttctccg gtaacaaatc ggtgaaagat gacatgctga
    40201 agcaaaacct cgaggcttct ggtgtgcgtg tgggcgaatc cctcgatcgc accaccattg
    40261 ccgatatcga gaaaggtctg gaagacttct actacagcgt cggtaaatat agcgccagcg
    40321 taaaagctgt cgtgaccccg ctgccgcgca accgtgttga cctaaaactg gtgttccagg
    40381 aaggtgtgtc agctgaaatc cagcaaatta acattgttgg taaccatgct ttcaccaccg
    40441 acgaactgat ctctcatttc caactgcgtg acgaagtgcc gtggtggaac gtggtaggcg
    40501 atcgtaaata ccagaaacag aaactggcgg gcgaccttga aaccctgcgc agctactatc
    40561 tggatcgcgg ttatgcccgt ttcaacatcg actctaccca ggtcagtctg acgccagata
    40621 aaaaaggtat ttacgtcacg gtgaacatca ccgaaggcga tcagtacaag ctttctggcg
    40681 ttgaagtgag cggcaacctt gccgggcact ccgctgaaat tgagcagctg actaagatcg
    40741 agccgggtga gctgtataac ggcaccaaag tgaccaagat ggaagatgac atcaaaaagc
    40801 ttctcggtcg ctatggttat gcctatccgc gcgtacagtc gatgcccgaa attaacgatg
    40861 ccgacaaaac cgttaaatta cgtgtgaacg ttgatgcggg taaccgtttc tacgtgcgta
    40921 agatccgttt tgaaggtaac gatacctcga aagatgccgt cctgcgtcgc gaaatgcgtc
    40981 agatggaagg tgcatggctg gggagcgatc tggtcgatca gggtaaggag cgtctgaatc
    41041 gtctgggctt ctttgaaact gtcgataccg atacccaacg tgttccgggt agcccggaac
    41101 aggttgatgt cgtctacaag gtaaaagagc gcaacaccgg tagcttcaac tttggtattg
    41161 gttacggtac tgaaagtggc gtgagcttcc aggctggtgt gcagcaggat aactggttag
    41221 gtacaggtta tgctgttggt atcaacggga ccaaaaacga ttaccagacc tatgctgaac
    41281 tgtcggtaac caacccgtac ttcaccgtag atggcgtaag cctcggtggt cgtctcttct
    41341 ataatgactt ccaggcagat gacgccgacc tgtccgacta taccaacaag agttatggta
    41401 cagacgtgac gttgggcttc ccgattaacg aatataactc gctgcgtgca ggtctgggtt
    41461 atgtacataa ctccctgtcc aacatgcagc ctcaggttgc gatgtggcgt tatctgtact
    41521 ctatgggtga acatccgagc acctctgatc aggataacag cttcaaaacg gacgacttca
    41581 cgttcaacta tggttggacc tataacaagc ttgaccgtgg ttacttcccg acagatggtt
    41641 cacgtgtcaa cctgaccggt aaagtgacca ttcctggatc ggataacgaa tactacaaag
    41701 tgacgttaga cacggcgact tatgtgccga tcgatgacga tcacaaatgg gttgttctgg
    41761 gtcgtacccg ctggggttat ggtgatggtt taggcggcaa agagatgccg ttctacgaga
    41821 acttctatgc cggtggttcc agcaccgtgc gtggcttcca gtccaatacc attggtccga
    41881 aagcagttta cttcccgcat caggccagta attatgatcc ggactatgat tacgaatgtg
    41941 cgactcagga cggcgcgaaa gacctgtgta aatcggatga tgctgtaggc ggtaacgcca
    42001 tggcggttgc cagcctcgag ttcatcaccc cgacgccgtt tattagcgat aagtatgcta
    42061 actcggttcg tacttccttc ttctgggata tgggtaccgt ttgggataca aactgggatt
    42121 ccagccaata ttctggatat ccggactata gtgatccaag caatatccgt atgtctgcgg
    42181 gtatcgcatt acaatggatg tccccattgg ggccgttggt gttctcctac gcccagccgt
    42241 tcaaaaagta cgatggagac aaggcagaac agttccagtt taacatcggt aaaacctggt
    42301 aagtgttctc cacaaaggaa tgtagtggta gtgtagcgat gactttaggc gatcaatata
    42361 agatcgccgg gccacgcaaa gaactgcacc ctccggtgca aatgggatgg taaggagttt
    42421 attgtgaaaa agtggttatt agcggcaggt ctcggtttag cactggcaac ttctgctcag
    42481 gcggctgaca aaattgcaat cgtcaatatg ggcagcctgt tccagcaggt agcgcagaaa
    42541 accggtgttt ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga actgcagcgt
    42601 atggaaaccg atctgcaggc taaaatgaaa aagctgcagt ccatgaaagc gggcagcgat
    42661 cgcactaagc tggaaaaaga cgtgatggct cagcgccaga cttttgctca gaaagcgcag
    42721 gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact ggttactcgt
    42781 atccagactg ctgtgaaatc cgttgccaac agccaggata tcgatctggt tgttgatgca
    42841 aacgccgttg cttacaacag cagcgatgta aaagacatca ctgccgacgt actgaaacag
    42901 gttaaataag taatgccttc aattcgactg gctgatttag cgcagcagtt ggatgcagaa
    42961 ctacacggtg atggcgatat cgtcatcacc ggcgttgcgt ccatgcaatc tgcacaaaca
    43021 ggtcacatta cgttcatggt taacccaaaa taccgtgagc atttaggctt gtgccaggcg
    43081 tccgcggttg tcatgaccca ggacgatctt cctttcgcga aaagtgccgc gctggtagtg
    43141 aagaatccct acctgactta cgcgcgcatg gcgcaaattt tagataccac gccgcagccc
    43201 gcgcagaaca ttgcacccag tgcggtgatc gacgcgacgg cgaagctggg taacaatgta
    43261 tcgattggcg ctaacgcggt gattgaatcc ggcgttgaac tgggcgataa cgtgattatc
    43321 ggtgccggtt gcttcgtagg taaaaacagc aaaatcggtg caggttcgcg tctctgggcg
    43381 aacgtaacca tttaccatga gatccagatc ggtcagaatt gcctgatcca gtccggaaca
    43441 gtggtaggcg cagacggctt tggttatgcc aacgatcgtg gtaactgggt gaagatccca
    43501 cagattggtc gcgtaattat tggcgatcgc gtggagatcg gtgcctgcac aaccatcgat
    43561 cgcggcgcgc tggatgacac tgttattggc aatggcgtga tcattgataa ccagtgccag
    43621 attgcacata acgtcgtgat tggcgacaat acggcggttg ccggtggcgt cattatggcg
    43681 ggcagcctga aaattggtcg ttactgcatg atcggcggag ccagcgtaat caacgggcat
    43741 atggaaatat gcgacaaagt gacggttacg ggcatgggta tggtgatgcg tcccatcact
    43801 gaaccaggcg tctattcctc aggcattccg ctgcaaccca acaaagtctg gcgcaaaacc
    43861 gctgcactgg tgatgaacat tgatgacatg agcaagcgtc tgaaatcgct tgagcgcaag
    43921 gttaatcaac aagactaacg ttccaatctt ttgttcgcca aactttacgg cctgtctcat
    43981 tcttacgatt gcggcaggcc gtgttattat tgtcgtttct tatattttga caggaagagt
    44041 atcttgacta ctaacactca tactctgcag attgaagaga ttttagaact tctgccgcac
    44101 cgtttcccgt tcttactggt ggatcgcgtg ctggattttg aagaaggtcg ttttctgcgc
    44161 gcagtaaaaa atgtctctgt caatgagcca ttcttccagg gccatttccc tggaaaaccg
    44221 attttcccgg gtgtgctgat tctggaagca atggcacagg caacaggtat tctggcgttt
    44281 aaaagcgtag gaaaactgga accgggtgag ctgtactact tcgctggtat tgacgaagcg
    44341 cgtttcaagc gcccggtcgt gcctggcgat caaatgatta tggaagtcac tttcgaaaaa
    44401 acgcgccgcg gcctgacccg ttttaaaggg gttgctctgg tcgatggtaa agtagtttgc
    44461 gaagcaacga tgatgtgtgc tcgtagccgg gaggcctgat acgtgattga taaatccgcc
    44521 tttgtgcatc caaccgccat tgtggaagag ggcgcgtcaa ttggcgcgaa cgcacacatt
    44581 ggtccttttt gtatcgttgg accccatgtc gaaattggtg agggtaccgt actgaaatct
    44641 cacgttgtcg tgaatggtca tactaaaatt ggccgcgata atgagattta tcagttcgcc
    44701 tccatcggcg aagttaacca ggatttgaaa tatgctggcg aaccgacccg tgtggaaatc
    44761 ggcgatcgta accgcattcg cgaaagcgtc accattcatc gtggcacagt ccagggcggt
    44821 ggattgacga aggtgggcag cgacaactta ctgatgatca acgcgcacat tgcgcacgat
    44881 tgtacggtag gtaaccgctg tattctcgcc aacaacgcaa cgctggcggg tcacgtatcg
    44941 gttgacgact tcgcgatcat cggcggcatg accgcagtcc atcagttctg catcattggt
    45001 gcgcatgtga tggttggcgg ttgctccggt gtggcgcagg acgtccctcc ttatgtcatt
    45061 gcgcagggta accacgcaac gccgttcggt gtcaatatcg aagggctgaa gcgccgcgga
    45121 ttcagccgtg aggcgattac cgctatccgc aatgcgtata agctgattta tcgtagcggt
    45181 aaaacgctcg atgaagtgaa accggaaatt gctgaactgg cggaaacata tccggaagtg
    45241 aaagccttta ccgatttctt ttcacgctca acgcgcggtc tgattcgtta atgactgaac
    45301 agcgtccatt aacgattgcc ctggtcgccg gagaaacctc cggcgatatc ctgggggccg
    45361 gtttaatccg cgctctgaaa gaacgtgtgc ccaacgcccg ctttgttggt gttgccgggc
    45421 cacgaatgca ggctgaaggc tgcgaagcct ggtacgaaat ggaagaactg gcggtgatgg
    45481 gcattgttga agtgctcggt cgtctgcgtc gcttactgca tattcgtgcc gatctgacaa
    45541 agcgttttgg cgaactgaag ccagatgttt ttgttggtat tgatgcgcct gacttcaata
    45601 ttactcttga aggtaacctc aaaaagcagg gtatcaaaac cattcattac gtcagtccgt
    45661 cagtctgggc gtggcgacag aaacgtgttt tcaaaatagg cagagccacc gatctggtgc
    45721 tcgcatttct gcctttcgaa aaagcgtttt atgacaaata caacgtaccg tgccgcttta
    45781 tcggtcatac catggctgat gccatgccat tagatccaga taaaaatgcc gcccgtgatg
    45841 tgctggggat ccctcacgat gcccactgcc tggcgttgct accggggagc cgtggtgcag
    45901 aagttgaaat gcttagtgcc gatttcctga aaacggccca gcttttgcgc cagacatatc
    45961 cggatctcga aatcgtggtg ccactggtga atgccaaacg ccgcgagcag tttgaacgca
    46021 tcaaagctga agtcgcgcca gacttttcag ttcatttgct ggatgggatg ggccgtgagg
    46081 cgatggtcgc cagcgatgcg gcgctactgg cgtcgggtac ggcagccctg gagtgtatgc
    46141 tggcgaaatg cccgatggtg gtgggatatc gcatgaagcc ttttaccttc tggttggcga
    46201 agcggctggt gaaaactgat tatgtctcgc tgccaaatct gctggcgggc agagagttag
    46261 tcaaagaatt attgcaggaa gagtgtgagc cgcaaaaact ggctgcggcg ctgttaccgc
    46321 tgttggcgaa cgggaaaacc agccacgcga tgcacgatac cttccgtgaa ctgcatcagc
    46381 agatccgctg caatgccgat gagcaggcgg cacaagccgt tctggagtta gcacaatgat
    46441 cgaatttgtt tatccgcaca cgcagctggt tgcgggtgtg gatgaagtcg gacgcgggcc
    46501 gttagttggc gcggtcgtca ccgctgcggt tattctcgac ccggcgcgcc cgattgccgg
    46561 gctgaatgat tccaaaaagc tgagcgaaaa acgccgtctg gcgctctgcg aagagatcaa
    46621 agagaaagcg ttgagctgga gtctgggccg cgcggaaccc cacgaaatcg acgagctgaa
    46681 cattcttcat gcgaccatgc tggcgatgca gcgtgccgtc gctgggctgc atattgcgcc
    46741 ggaatatgtg ttgattgatg gtaaccgctg cccgaaatta ccgatgcctg cgatggctgt
    46801 ggtgaaaggc gatagccgcg taccggaaat cagtgccgcg tctatcctgg cgaaagtgac
    46861 gcgtgacgcc gaaatggcgg cgctggatat tgttttcccg caatatggtt ttgcccaaca
    46921 caaagggtac ccaaccgctt ttcatctgga aaaactggct gaacacggcg cgaccgaaca
    46981 ccatcggcgc agctttgggc ctgtcaaacg cgcactggga cttgcgtcct gattcttgtg
    47041 tcgagattaa gtaaaccgga atctgaagat gtctgaacca cgtttcgtac acctgcgggt
    47101 gcacagcgac tactcgatga tcgatggcct ggccaaaacc gcaccgttgg taaaaaaggc
    47161 ggcggcgttg ggtatgccag cactggcgat caccgatttc accaaccttt gtggtctggt
    47221 gaagttctac ggagcgggac atggcgcagg gattaagcct atcgtcgggg cagattttaa
    47281 cgtccagtgc gacctgctgg gtgatgagtt aacccacctg acggtactgg cggcgaacaa
    47341 taccggctat cagaatctga cgttgctgat ctcaaaagcg tatcagcgcg ggtacggtgc
    47401 cgctgggccg atcatcgatc gcgactggct tatcgaatta aatgaagggt tgatccttct
    47461 ttccggcggg cgcatgggcg acgtcggacg cagtcttttg cgtggtaaca gcgcgctggt
    47521 agatgagtgt gtcgcgtttt atgaagaaca cttcccggat cgctattttc tcgagctgat
    47581 ccgcaccggc aggccggatg aagaaagcta tctgcacgcg gcggtggaac tggcggaaac
    47641 gcgcggtttg cccgtcgtgg cgactaacga tgtgcgcttt atcgacagca gcgactttga
    47701 cgcacacgaa atccgcgtcg cgatccacga cggctttacc ctcgacgatc ctaaacgccc
    47761 gcgtaactat tcgccgcagc aatatatgcg tagcgaagag gagatgtgtg agctgtttgc
    47821 cgacatcccc gaagcccttg ccaacaccgt tgagatcgcc aaacgctgta acgtaactgt
    47881 gcgtcttggt gaatacttcc tgccgcagtt cccgaccggg gacatgagca ccgaagatta
    47941 tctggtcaag cgtgcaaaag agggcctgga agagcgtctg gcctttttat tccctgacga
    48001 ggaagaacgt gttaagcgcc gcccggaata tgacgaacgt ctggagactg aacttcaggt
    48061 tatcaaccag atgggcttcc cgggctactt cctcatcgtt atggaattta tccagtggtc
    48121 gaaagataac ggcgtaccgg tagggccagg ccgtggctcc ggtgcgggtt cactggtggc
    48181 ctacgcgctg aaaatcaccg acctcgatcc gctggaattt gacctgctgt tcgaacgttt
    48241 ccttaacccg gaacgtgtct ccatgcctga cttcgacgtt gacttctgta tggagaaacg
    48301 cgatcaggtt atcgagcacg tagcggacat gtacggtcgt gatgcggtat cgcagatcat
    48361 caccttcggt acaatggcgg cgaaagcggt gatccgcgac gtaggccgcg tgctggggca
    48421 tccgtacggc tttgtcgatc gtatctcgaa actgatcccg cccgatccgg ggatgacgct
    48481 ggcgaaagcg tttgaagccg agccgcagct gccggaaatc tacgaagcgg atgaagaagt
    48541 taaggcgctg atcgacatgg cgcgcaaact ggaaggggtc acccgtaacg ccggtaagca
    48601 cgccggtggg gtggttatcg cgccgaccaa aattaccgat tttgcgccgc tttactgcga
    48661 tgaagagggc aaacatccgg tcacccagtt tgataaaagc gacgttgaat acgccggact
    48721 ggtgaagttc gacttccttg gtttgcgtac gctcaccatc atcaactggg cgctggagat
    48781 gatcaacaag cggcgggcga agaatggcga gccgccgctg gatatcgccg cgatcccgct
    48841 ggatgacaag aaaagcttcg acatgctgca acgctcggaa accacggcgg tattccagct
    48901 tgaatcgcgc ggcatgaagg acctgatcaa gcgtctgcaa cctgactgct tcgaagatat
    48961 gatcgcactg gtggcgctgt tccgcccagg tccgttgcaa tcagggatgg tggataactt
    49021 tatcgaccgt aaacatggtc gcgaagaaat ctcctatccg gacgtacagt ggcagcatgg
    49081 aagcctgaaa ccggtactgg agccaaccta cggcatcatc ctgtatcagg aacaggtcat
    49141 gcagattgcg caggtgcttt ctggttatac cctcggtggc gcggatatgc tgcgtcgtgc
    49201 gatgggtaag aaaaagccgg aagagatggc taagcagcgt tctgtatttg ctgaaggtgc
    49261 agaaaagaac ggaatcaacg ccgaactggc gatgaaaatc ttcgacctgg tggagaaatt
    49321 cgcgggttac ggatttaaca aatcgcactc tgcggcctat gctttggtgt catatcaaac
    49381 gttatggctg aaagcgcact atccggcgga gtttatggcg gcggtaatga ccgccgatat
    49441 ggacaacacc gagaaggtgg tgggcctggt ggatgagtgc tggcggatgg ggctgaaaat
    49501 cctgccacca gatataaact ccggtcttta ccatttccac gtcaacgacg acggcgaaat
    49561 cgtgtatggt attggcgcca tcaaaggggt aggtgaaggt ccgattgagg ccatcatcga
    49621 agcccgtaat aaaggcggct acttccgcga actgtttgat ctctgcgccc gtaccgacac
    49681 caaaaagtta aaccggcggg tgctggaaaa actgatcatg tccggggcgt ttgaccgtct
    49741 tgggccacac cgcgcggcgc tgatgaactc gctgggtgat gcgttaaaag cggcagatca
    49801 acacgcgaaa gcggaagcta tcggtcaggc cgatatgttc ggcgtgctgg ccgaagagcc
    49861 ggaacaaatt gaacaatcct acgccagctg ccaaccgtgg ccggagcagg tggtgttaga
    49921 tggggaacgt gaaacgttag gtctgtacct tacgggacac cctatcaacc agtatttaaa
    49981 agagattgag cgttatgtcg gaggcgtaag gctgaaagac atgcacccga cagaacgtgg
    50041 taaagtcatc acggctgcgg ggctcgttgt tgccgcgcgg gttatggtca ccaagcgcgg
    50101 caatcgtatc ggtatctgca cgctggatga ccgttccggg cggctggaag tgatgttgtt
    50161 tactgacgcc ctggataaat accagcaatt gctggaaaaa gaccgcatac ttatcgtcag
    50221 cggacaggtc agctttgatg acttcagcgg tgggcttaaa atgaccgctc gcgaagtgat
    50281 ggatattgac gaagcccggg aaaaatatgc tcgcgggctt gctatctcgc tgacggacag
    50341 gcaaattgat gaccagcttt taaaccgact ccgtcagtct ctggaacccc accgctctgg
    50401 gacaattcca gtacatctct actatcagag ggcggatgca cgcgcgcggt tgcgttttgg
    50461 cgcgacgtgg cgtgtctctc cgagcgatcg tttattaaac gatctccgtg gcctcattgg
    50521 ttcggagcag gtggaactgg agtttgacta atacaggaat actatgagtc tgaatttcct
    50581 tgattttgaa cagccgattg cagagctgga agcgaaaatc gattctctga ctgcggttag
    50641 ccgtcaggat gagaaactgg atattaacat cgatgaagaa gtgcatcgtc tgcgtgaaaa
    50701 aagcgtagaa ctgacacgta aaatcttcgc cgatctcggt gcatggcaga ttgcgcaact
    50761 ggcacgccat ccacagcgtc cttataccct ggattacgtt cgcctggcat ttgatgaatt
    50821 tgacgaactg gctggcgacc gcgcgtatgc agacgataaa gctatcgtcg gtggtatcgc
    50881 ccgtcttgac ggtcgtccgg ttatgatcat tggtcatcaa aaaggtcgtg aaaccaaaga
    50941 aaaaattcgc cgtaactttg gtatgccagc gccagaaggt taccgcaaag cactgcgtct
    51001 gatgcaaatg gctgaacgct ttaagatgcc tatcatcacc tttatcgaca ccccgggggc
    51061 ttatcctggc gtgggcgcag aagagcgtgg tcagtctgaa gccattgcac gcaacctgcg
    51121 tgaaatgtct cgcctcggcg taccggtagt ttgtacggtt atcggtgaag gtggttctgg
    51181 cggtgcgctg gcgattggcg tgggcgataa agtgaatatg ctgcaataca gcacctattc
    51241 cgttatctcg ccggaaggtt gtgcgtccat tctgtggaag agcgccgaca aagcgccgct
    51301 ggcggctgaa gcgatgggta tcatcgctcc gcgtctgaaa gagctgaaac tgatcgactc
    51361 catcatcccg gaaccgctgg gcggcgctca ccgtaacccg gaagcgatgg cggcatcgtt
    51421 gaaagcgcaa ctgctggcgg atctggccga tctcgatgtg ttaagcactg aagatttaaa
    51481 aaatcgtcgt tatcagcgcc tgatgagcta cggttacgcg taattcgcaa aagttctgaa
    51541 aaagggtcac ttcggtggcc cttttttatc gccacggttt gagcaggcta tgattaagga
    51601 aggattttcc aggaggaaca catgaacatc attgccatta tgggaccgca tggcgtcttt
    51661 tataaagatg agcccatcaa agaactggag tcggcgctgg tggcgcaagg ctttcagatt
    51721 atctggccac aaaacagcgt tgatttgctg aaatttatcg aacataaccc tcgaatttgc
    51781 ggcgtgattt ttgactggga tgagtacagt ctcgatttat gcagcgatat caatcaactt
    51841 aatgaatatc tcccgcttta tgccttcatc aacacccact cgacgatgga tgtcagcgtg
    51901 caggatatgc ggatggcgct ctggtttttt gaatatgcgc tggggcaggc ggaagatatc
    51961 gccattcgta tgcgtcagta caccaacgaa tatcttgata acattacgcc gccgttcacg
    52021 aaagccttgt ttacctacgt taaagagcgg aagtacacct tttgtacgcc ggggcatatg
    52081 ggcggcaccg catatcaaaa aagcccggtt ggctgcctgt tttatgattt tttcggcggg
    52141 aataccctca aggctgatgt ctctatttcg gtcaccgagc ttggttcgtt gctcgaccac
    52201 accgggccac acctggaagc ggaagagtac atcgcgcgga cttttggcgc ggaacagagt
    52261 tatatcgtta ccaacggaac atcgacgtcg aacaaaattg tgggtatgta cgccgcgcca
    52321 tccggcagta cgctgttgat cgaccgcaat tgtcataaat cgctggcgca tctgttgatg
    52381 atgaacgatg tagtgccagt ctggctgaaa ccgacgcgta atgcgttggg gattcttggt
    52441 gggatcccgc gccgtgaatt tactcgcgac agcatcgaag agaaagtcgc tgccaccacg
    52501 caggcacaat ggccggttca tgcggtgatt accaactcca cctatgatgg cttgctctac
    52561 aacaccgact ggatcaaaca gacgctggat gtcccgtcga tccacttcga ttccgcctgg
    52621 gtgccgtaca cccattttca tccgatctac caggggaaaa gtggtatgag cggcgagcgt
    52681 gttgcgggaa aagtgatctt cgaaacgcaa tcgacccaca aaatgctggc ggcgttatcg
    52741 caggcttcgc tgatccacat taaaggcgag tatgacgaag aggcgtttaa cgaagccttt
    52801 atgatgcata ccaccacctc gcccagttat cccattgttg cttcggttga gacggcggcg
    52861 gcgatgctgc gtggtaatcc gggcaaacgg ctgattaacc gttcggtgga gcgggcgctg
    52921 cattttcgca aagaggtcca gcggttgcgg gaagagtctg acggttggtt tttcgatatc
    52981 tggcaaccgc cgcaggtgga tgaagccgaa tgctggcccg ttgcgccagg cgaacagtgg
    53041 cacggcttta acgatgcgga tgccgatcat atgtttctcg atccggttaa agtcactatt
    53101 ttgacaccgg ggatggacga acagggcaat atgagcgagg aggggatccc ggcggcgctg
    53161 gtggcaaaat tcctcgacga acgtgggatc gtagtagaga aaaccggccc ttataacctg
    53221 ctgtttctct ttagtattgg tatcgataaa accaaagcaa tgggattatt gcgtgggttg
    53281 acggaattta aacgctctta cgatctcaac ctgcggatca aaaatatgct acccgatctc
    53341 tatgctgaag atcccgattt ctaccgcaat atgcgtattc aggatctggc gcaagggatc
    53401 cataagctga ttcgtaaaca cgatcttccc ggtttgatgt tgcgggcatt cgatactttg
    53461 ccggaaatga tcatgacgcc acatcaggca tggcagagac agattaaagg tgaagtagaa
    53521 accattgcgc tggaacaact ggtcggtaga gtatcggcaa atatgatcct gccttatcca
    53581 ccgggcgtac cgctgttgat gccaggagaa atgctgacca aagagagccg cacggtactc
    53641 gattttctac tgatgctttg ttccgtaggg caacattacc ccggttttga aacggatatt
    53701 cacggcgcga aacaggacga agacggcgtt taccgcgtac gagtcctaaa aatggcggga
    53761 taacttgcca gagcggcttc cgggcgagta acgtgctgtt aacaaataaa ggagacctta
    53821 tgctgggttt aaaacaggtt caccatattg cgattattgc gacggattat gcggtgagca
    53881 aagctttcta ctgcgatatt cttggtttca cgctgcaaag cgaagtctat cgcgaagcgc
    53941 gcgactcgtg gaaaggggat ttggcgctta atgggcaata tgtgattgag cttttctctt
    54001 ttccgttccc gccggagcgc cccagccgac cggaagcttg cggcctgcgt catctggctt
    54061 ttagcgttga tgacatcgat gcggcagtgg cgcaccttga aagccataac gtgaagtgtg
    54121 aagccatccg tgtcgatcca tacacgcaaa aacgcttcac cttctttaac gatccggacg
    54181 ggctgccgtt ggaactgtat gagcagtaag gcttgtcatc gccgcatttg cccggtaacg
    54241 tgccgggcat tgctactgta aaatcgcacc atcatgacac tcacgctcaa tagacaactt
    54301 ctcacctcac gccagattct ggtggccttt agcggcgggc ttgactccac cgttctgctg
    54361 catcagttgg tgcagtggcg gacggaaaat ccgggcgtca ctctgcgcgc tattcatgtg
    54421 catcacggtt taagtgccaa tgccgatgcc tggtttacgc attgcgaaaa cgtctgccaa
    54481 cagtggcagg tgccgctggt ggtcgaacgc gtacaacttg cgcaagaggg gctggggatt
    54541 gaggcccaag cgcggcaggc gcgttatcag gcatttgccc gtaccctatt gcccggcgaa
    54601 gtgctggtca ccgcgcaaca tctcgacgat caatgtgaaa cctttctgct ggcgctaaaa
    54661 cgcggtagcg gccctgccgg gctttcggct atggcggaag tttcggagtt tgccggaaca
    54721 cggcttatcc gcccgttgct tgcccgcacg cggggggaac tggcgcagtg ggcattggcg
    54781 catggtttac gctggattga agacgaaagt aatcaggacg actcatacga tcgtaacttt
    54841 ctgcgcctgc gcgtagtgcc gttattgcag cagcgttggc cgcattttgc ggaagcaacg
    54901 gcccgcagcg ccgcactttg tgctgaacaa gagagtctgt tggatgaact gctggcggat
    54961 gatttagcac actgccaaac gtcgcagggg acgctgcaga ttgcgccaat gctggcgatg
    55021 agtgatgccc gccgcgcggc gattatccgt cgctggctgg cagggcagaa tgcaccaatg
    55081 ccttcccgcg acgcgctggt gcggatctgg caggaagtgg cgctggcgcg ggaagatgcc
    55141 tccccctgtt tacgcttggg cgcgtttgaa atccgccgct atcagtcgca actgtggtgg
    55201 attaaatccg tcaccgggca aagtgaaacc attgtgccgt ggcagacgtg gctacaaccg
    55261 ctggaattac cggcggggct gggaagcgta cagcttactg cgggaggcga tattcgccct
    55321 ccgcgtgcag acgaagcggt cagcgtgcgt ttcaaagcgg caggattgct gcatattgtc
    55381 gggcgcaacg gcggacgtaa gctgaagaaa atctggcaag agttaggcgt gccgccgtgg
    55441 ctacgtgaca ccacgccact gctgttttat ggcgaaacgc tgattgcggc ggcaggggta
    55501 tttgtgacgc aagaaggtgt ggctgaaggt gagaatggcg taagttttgt ctggcagaaa
    55561 acgcttagtt aagtgaaagc cggataagac gcatcaaacg tcgcatccgg cgaaagtcaa
    55621 tcaggactcg ctcaccacca ccgtaccgat ttccgggtgg ctaaagctgg taattttatc
    55681 cagacgtagc tcacgggttt cgccagcggc ctcgacgacc agatactcca catttttgcg
    55741 ggagactaaa tcgctggctt ttgcctgcaa tttttcgcca tctttcagct caagtgtcag
    55801 cattaaatga tgctggcagg cgagctcaag attatcgtaa tcatcacaat tgattggttg
    55861 atacgtatca ttcattgaca taatcgctca ccagtaagtt tgccgcagcg tatgctgctt
    55921 tttccctgac agcctcagaa agggcgtcgt cggcagccat ttcattcagc actttcaaaa
    55981 cgcagcccag cgcgtccgga acgtatccta agtctccgct ggcgatttcc gcgtaccgct
    56041 tgcgtattaa ctcacaatat ttttccacat gccctcctgt cagcactctg acttaaccgt
    56101 ggatgcaagt ctaagcctac gaagataaac tctgtttcgc aaggtgacta taccacactc
    56161 atttctgcaa tatcagcgcc gcaactgcac gtattccgtt acaatggcct cctgattcga
    56221 aaggagtttt cttatggcgc ttaaagcgac aatttataaa gcgacggtta atgtggccga
    56281 tctcgaccgc aaccagtttc tcgatgcctc tctgacgctg gcgcgccacc cttcagaaac
    56341 ccaggagcgt atgatgctgc gcttgctggc gtggctgaaa tatgctgatg aacgtctgca
    56401 atttacccgt ggtttgtgtg ccgatgatga gccggaagcg tggctgcgta acgatcacct
    56461 gggcattgat ttgtggattg agctggggct gccggatgag cggcggatta agaaagcctg
    56521 cacccaggcc gcagaagtgg cgctgtttac ctataatagt cgggcggcgc aaatctggtg
    56581 gcagcaaaat cagagcaaat gtgtgcagtt tgccaatctt tccgtctggt atctggacga
    56641 tgaacaactg gcgaaagtaa gcgcctttgc cgatcgtacc atgacgctgc aggcaacgat
    56701 tcaggatggc gtgatctggt tatcggacga taagaataat ctgaaagtta acttaaccgc
    56761 ctggcaacaa ccttcatgat tgtgatttcc cgacatgttg ctattcccga tggtgagctt
    56821 gagatcaccg ccattcgtgc gcagggcgcg ggcgggcagc atgttaataa gacctcaacg
    56881 gccattcatc tgcgttttga cattcgggcg tccagcctgc cagagtatta caaagagcgt
    56941 ctgctcgccg ccagccatca tttgatcagc agtgatggcg tgattgtcat taaggcacag
    57001 gaataccgca gtcaggaact gaaccgcgaa gccgcgctgg cccggctggt ggcagtgatt
    57061 aaagatttaa caacagaaca aaaagcccga cgacccacgc ggcccacccg tgcatcgaaa
    57121 gagcgcaggc tggcatcgaa agcacaaaaa tcaagcgtga aggcgatgcg cggcaaagtg
    57181 cgcagcggtc gggaataaaa agaaggaatg gatggtgaaa aaagcgatag tgacagcgat
    57241 ggctgtaatc agcctcttta ctctgatggg atgtaataat cgggccgaag ttgatgcgct
    57301 ttctccggcg caggctgccg aactgaaacc gatgccgcaa agttggcgcg gcgtgctgcc
    57361 gtgtgctgat tgcgaaggaa tcgaaacctc tctgttcctc gaaaaagacg gaacatgggt
    57421 gatgaatgag cgttatctcg gtgctcgtga agaaccttcc tccttcgctt cctacggtac
    57481 atgggcgcga accgctgaca agctggtatt aaccgacagc aaaggtgaaa agtcatatta
    57541 tcgggcgaaa ggcgatgcgc tggagatgct cgatcgtgaa ggcaatccga ttgaatcgca
    57601 gttcaactat acgctggaac cggcacaatc cagtttacct atgacgccga tgaccctgcg
    57661 aggcatgtat ttttatatgg ctgatgcggc gaccttcact gattgcgcga ccggaaaacg
    57721 tttcatggta gcgaataacg cagagctgga gcgtggctac ctggctgcgc gcggtcacag
    57781 tgaaaaaccg gtgttactgt cagtagaagg tcactttacg cttgaggcta atccggatac
    57841 tggtgcgcca accaaagtat tagcgcccga tacggcgggt aaattttacc cgaataagga
    57901 ttgcagtagt ttggggcagt aaacctgctt aaggcagaaa caaacgcaaa attgcctgat
    57961 gcgctgcgct tatcaggcct acagcatata atgcaacatt ttgaattagc acgattttgt
    58021 aggccggata aggcgtttac gccgcatccg gcattttgtg cacgaggctg gctttatcac
    58081 tgagtcagcc caacaaagcg acccgccttg atataaatcc caggcttcag gtgcccaaca
    58141 tactgtaact gcgtttctgg cttaaacgca gagacatcac caatacgcat atgcatcaga
    58201 tcggcagggc taatccagcc cgattgcgcc agtgtaagtg gatgcccggc tttggcaaag
    58261 gcatccgtga caaattccga acaaaaccac gactttttgt ctccttcgcc cacactgctt
    58321 aactgcgctt tcgccaggcc gctgacgcac tttggcgaaa atcctcggag aacggattca
    58381 gtgagcacat ctggcgagtc accataaagg gaataaattc gacaatgccg cgatagttat
    58441 aaccgctatc tttgatttta ttggcaaacg cggtgatatc tgtggcttgt tgcggggtaa
    58501 gatccgggac tcgtaagacg aaaagcttat cactatgctt catcgctttt ttaagggaaa
    58561 caatctggac gccagcgcct gtcgcttctg caacgttatt atcacccaga aagattgcaa
    58621 cgtgactcac agaggaagtg ctgaagacgc ggattccaaa tgaggttacc ccaaggcttg
    58681 aggagaacag caaatcgccg ggtttgagat caggtgccgt tatttctttt attgattgtt
    58741 cggtgaaaga gctttgatgc tggaatttga cagcccatgt ttttgcctcg gcatctactg
    58801 ctgtggctga tgggtctggc tggctgatat caacggtaca agccgaaagt aaaagaaagc
    58861 tggggagaag caggcggtag tacgcctttg gtttatccat tttatacaat ccatgtaaaa
    58921 aagggccctg aaattcagga ccctttctgg catcagcctt taatctgttt caccagataa
    58981 tcgacgatgt caccagtctt aattaactgt ttctcgccgt tacgacgata tttatattcg
    59041 atatcgtcgt tgtcgaggtt acggtcgccc agcacaatag tgtgcggaat accgatcagt
    59101 tccatatcag caaacatcac gcccggacgc tctttgcggt catccagcag cacttcgata
    59161 ccttgtgcac gcagttcgct gtacagtttc tcagcaagct cttgtacgcg gaaggatttg
    59221 tgcatgttca tcggcagaat cgccacctgg aacggcgcga tagcgtcagg ccatacgatg
    59281 cctcgttcgt cgtagttctg ctcaatcgcc gcagctacca cacgcgttac cccgataccg
    59341 tagcaaccca tcgtcaggat ttggttacgg ccatcttcac cctgtacgga ggctttcagt
    59401 gcttcggagt acttggtacc cagctggaag atgtgaccaa cttcgatacc acgtttgatc
    59461 agcagcgtac cctggccatc cgggcttgga tcgccagcca ccacgttacg gatatctgca
    59521 acttccgggg tagcgacatc gcgatcccag ttgatgccga agtagtgttt accatcgatg
    59581 ttagcaccag cagcgaaatc actcatcgcc gcaacggtac ggtcaatcac caccggaatc
    59641 ggcatgttta ccggacccag tgaacccgga ccggctttaa ccacggcacg aatttcttct
    59701 tcggtcgcga aagtcagcgg gcttgcaacc tgcggcagtt tttctgcttt aacttcgttc
    59761 agctcgtgat caccgcgcac cagcagcgca accagcggga agctactgcc ttcaaccgct
    59821 ttaaccagca gagtcttaac cgttttctca atcggcagat tgaactgttc aaccagttcc
    59881 gcgatggttt tcgcgttcgg cgtatcaacc agcgtcattt cctgggtagc agcagcgcgc
    59941 ggttctttcg gcgcgatagc ttctgccagt tcaatgttcg ctgcatagtc agaggtgtcg
    60001 gagaagacca catcgtcttc accgctctgc gccagcacct ggaattcgtg agaggcgctg
    60061 ccgccgatag aaccggtgtc ggcctgtacg gcgcggaaat ccagccccat gcggctgaag
    60121 attttgctgt aggccgcata cattgcatcg taggtttcct gcagggattc ctgagaagta
    60181 tggaaagagt aagcatcttt cataaggaat tcgcgggaac gcatgacgcc gaaacgcgga
    60241 cgcacttcgt cgcggaactt ggtctggatc tgatagaagt tcagcggcag ctgtttgtaa
    60301 gagctaagct cgttacgaat caggtcagtg ataacttctt catgagttgg gccgagtacg
    60361 aacggacgct cgccacggtc aacaaaacgc agcagttccg gaccgtactg ttcccaacga
    60421 ccactctctt gccacaaatc ggctggctga accaccggca tcgacacctc gattgcaccg
    60481 gcgttgttca tctcttcacg cacgatgttt tcgacttttt tcagaacgcg cacgccggtc
    60541 ggcagccagg tatataaccc ggaggccagc ttgcggatca tcccggcgcg cagcatcagc
    60601 tgatggctga tcacctcggc gtcggcaggt gtctccttga gagtggagag caggtattgg
    60661 ctagtacgca tgttgttacg gttccagttg gaaggtagaa caggctcaag gcgagcctgg
    60721 gacaaaaaaa gtgatttagt ttaccagtgc aaaagaaatg tcaaaagaga agggcgtgaa
    60781 tttaacgcgg ttccagcgca aagacttcaa aacctgcgtc ggtgacgcgc cagcgaacgt
    60841 taaaatcatg tagccagacg gcataggttt tgcccgtttc ctcaccttta cgataggccg
    60901 ggcgcgggtc ctgcgccagt acttcgcgga taaacagcgt taactgcgga taacgcttct
    60961 ccagcgtcaa aagctgcttt tcgacctctg cggtaaaact caccgccatc tctgccgctg
    61021 gcgcactttg cgcatagctg gcactggcat cgggaagcga ttcggcaaag gggagatacg
    61081 gtttgatatc cactaccggc gtaccatcga ccagatccag actgccgagc tttagaatca
    61141 cgctatcttt atggcaaaca acctctttca gctctaccag cgacatacca attgggttag
    61201 ggcggaaagt agagcgtgtt gcgaaaaccc ccattctggc gttaccgcca agacgaggcg
    61261 gacgcacagt cggacgccag ccgccttcca tcgtttgatg aaagacgaaa aggatccata
    61321 aatggctgaa cgcttccagg ccgcgaacgg cgtcggcctg gttgtaggga gcaatgagat
    61381 gcagttcgcc gttggcgctt tttaccagac ctggctggcg cggaacggcg aacttttctt
    61441 tatagggcga gcgaataacg cctatttgct cgaactggaa actgctcatt tcgccgtaat
    61501 gttaagcgca gaaccgatac atacagcctg acgatagcag cctggcgtac cgctggtgac
    61561 ttcgcagcta tgcagtaata ccgcattggc tttcattttc gaggcgttga tttgcatccg
    61621 cttacgtgcg gttggaatgc tcggcggaga gtcctgatta gaggcctggc aagagtcgcc
    61681 actgacttcg ccgagatcgc ggaacggttt gccgactaat tcttctgcat tggtataaat
    61741 tcggaccggc gtggcgcgcg gcgctttcgg ttttgcaggc tccgctttcg gctggggtgc
    61801 agtgctttga acgggttcga caggggatct gcttaacatg gaacagccgc ttagcatgag
    61861 tgctactaaa cagatcggta aagcacgcat agtatttcct caatgtatga tcaaaacgtc
    61921 aatattgaat caggagcttg taaaaatgac aagacgggca agcgcccgtc ctgaatgata
    61981 ttacaaattg tggaaacagc ctaaaaatta ccagccttta acagcgccgc cgttaaacac
    62041 tttgtttgct gcttcgtaaa cttcgtcaga ctgataagcc tggacgaatt tcttcacgtt
    62101 ctcggcgtct ttgttatctt cacgcgttac gatcaggttt acgtacgggg agtctttatc
    62161 ttcgacaaag ataccgtctt tcgctggagt caggccaatc tggctggcat aggtggtatt
    62221 gataactgcc agagcgattt gcgcgtcgtc cagagatcga ggcagctgcg gtgcttccag
    62281 ctcaacaatt ttcagatttt ttgggttctc aacaacatca agaacggtcg gcagcaggcc
    62341 aacgccatct ttcagtttga tcaagcccac tttttgcagc agcagcagtg aacgaccaag
    62401 attagttgga tcgtttggca cggcaacctg cgaaccatcc tgcagttcat ccagtgattt
    62461 gattttcttg gagtaaccag caatcggata aacaaaagtg ttgcctactg cgaccagttt
    62521 gtagctacga tctttcagtt gctgatcaag gtacggttta tgctggaagg cgttggcgtc
    62581 gatatcgcct ttgctcaatg cttcgtttgg cagaacatag tcgttgaagg ttaccagctc
    62641 aacgtccagg ccatatttgt ctttcgcaac tttctgcgcg acttctgcta cctgctgttc
    62701 ggcaccaaca atcacgccga ctttaatgtg gtttggatct ttttcatcct gaccgcagcc
    62761 taccagtgcc agtgatccaa tcagggctcc cactgccgca aaggttttga atttgaacgc
    62821 cataccttat tccttcttca attatttatg ttgtgttgaa cgttacttgc gagtgacagc
    62881 ccggacgatg cggtcgcctg cgaactgaat taaataaacc agaatgacca gcaataccag
    62941 taccgtattc atcaccgtcg cgttgtagcc gatgtagcca tactgatagc caatctgacc
    63001 taaaccaccg gcaccgactg cgccacccat cgcggaataa ccgaccaggg taatcagggt
    63061 gatagttgcc gcattcacca gacccggcaa cgcttccggt aacagcactt tacggacgat
    63121 ctgcattggc gtggcaccca ttgcgcggga agcttcaatt aacccggttg ggatctccag
    63181 cagagcgttc tcgaccatac gggcaataaa cggtgctgca ccaacggtta acggaacaat
    63241 tgctgcctgc aaaccaatcg atgtaccgac aataacgcgg gtaaacggaa tcatccatac
    63301 cagcaagata atgaacggga tggaacggaa aatgttcaca atcgcagaaa cggtacgata
    63361 ctgcttcgcg ttagcaataa tttgccccgg acgcgtgaca taaagcagaa cgccaaccgg
    63421 cagaccaatc acaaagccaa aaaatccgga tacgaaggtc attgccagcg tttcccatac
    63481 gccacgaacc agcagccaca tcatcggctc agacataacc cagtacctct acttttacat
    63541 ggtgttcctg cagccaggca atggcggctt gcgtatcttg ttgtgtgccg tgcatttcag
    63601 tcagcatgat gccgaattta acgccaccgg cgtagtccat ctgcgcgcta ataatgttgt
    63661 tgttgacgtt gaaacgacgc gcggtttcag aaagcagtgg ggcatcgacc gattgaccgg
    63721 taaactccag acgcagcatc ggcacgcagt cagtaaatgg ctccgcttgc agacgttcct
    63781 ggtaatcttc cgggatatcc agatgcaggg tcgactgaat aaacttctgc gccagcggcg
    63841 ttttcggatg cgagaacact tcacttaccg tgtcctgctc gatcagttcg ccattgctga
    63901 tgaccgccac gcaatcacaa atacgcttca caacgtccat ttcgtgggtg atcaacagaa
    63961 tcgtcaaacc cagacggcgg ttaatgtctt tcagcagttc gagaatagaa cgtgtcgttg
    64021 ccgggtccag cgcgctggtg gcttcatcac acagcaatac tttgggattg ctggctaatg
    64081 cacgggcaat cgccacacgt tgtttctgcc caccggaaag attcgacggg tagctatcat
    64141 gcttatcgcc aagaccaacc aatgacagca attccgtcac gcgacgtttg atctcgtctt
    64201 tcggtgtgtt gtccagctcc agcggcagag ccacgttgcc aaaaacagta cgcgaagaga
    64261 gcaggttaaa atgctggaaa atcataccaa tctggcggcg agctttggtc aactcggatt
    64321 ctgacagcgt ggtcagttcc tggccatcga ccagcacgct accctcggtt gggcgctcca
    64381 gcaggtttac acaacgtata agcgtactct tacccgcgcc tgaggcaccg ataacgccat
    64441 aaatttgtcc agctggcaca tgcaggctga cgttgttcaa cgcctggatg gtgcgggtgc
    64501 cctggtggaa cactttggtg atattcgaaa gttttatcat tgattattta ttatcgtcat
    64561 taagttagtc gtggcatctc gaatgcctga aacgggcaac gccgtcaatg aaatggatgt
    64621 taaggcatcc agacgtctaa atcaatcagg tttatgcgaa gagcactttc ttgcaggtcg
    64681 aaacatgcga tactagcgtc ccatgcctta ttaaggagct ataaaaggtg gcgaagagcg
    64741 tacccgcaat ttttcttgac cgtgatggca ccattaatgt cgatcacggc tatgtccatg
    64801 agatcgacaa ctttgaattt atcgacggtg ttattgacgc catgcgcgag ctaaaaaaaa
    64861 tgggctttgc gctggtggta gtaaccaacc agtctggcat tgctcgcggt aaatttaccg
    64921 aagcacagtt tgaaacgctg accgagtgga tggactggtc gctggcggac cgagatgtcg
    64981 atctggatgg tatctattat tgcccgcatc atccgcaggg tagtgttgaa gagtttcgcc
    65041 aggtctgcga ttgccgcaaa ccacatccgg ggatgctttt gtcagcacgc gattatttgc
    65101 atattgatat ggccgcttct tatatggtgg gcgataaatt agaagatatg caggcagcgg
    65161 ttgcggcgaa cgtgggaaca aaagtgctgg tgcgtacggg taaacctatt acacctgaag
    65221 cagaaaacgc ggcagattgg gtgttaaata gcctggcaga cctgccgcaa gcgataaaaa
    65281 agcagcaaaa accggcgcaa tgattaaaag atgagcggtt gaaataaaaa tgcatttttc
    65341 cgcttgtctt cctgagccga ctccctataa tgcgcctcca tcgacacggc ggatgtgaat
    65401 cacttcacac aaacagccgg ttcggttgaa gagaaaaaat cctgaaattc agggttgact
    65461 ctgaaagagg aaagcgtaat atacgccacc tcgcgacagt gagctgaaag c
//
LOCUS       DAZKHC020000014        63944 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZKHC020000014 DAZKHC020000000
VERSION     DAZKHC020000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 63944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.014"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..535
                     /locus_tag="ACHOSM_RS05415"
                     /old_locus_tag="pgaptmp_001083"
     CDS             <1..535
                     /locus_tag="ACHOSM_RS05415"
                     /old_locus_tag="pgaptmp_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HGU4557655.1"
                     /translation="RELVGVMQWGYALNPNSGARVVTGTRNREYMELNRLWLHDCMPR
                     NSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVGSHESTFYELD
                     GEWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRARKRLNTKLFKV
                     QPYPKPQTVKTGLKESE"
     gene            complement(664..2670)
                     /gene="ycjT"
                     /locus_tag="ACHOSM_RS05420"
                     /old_locus_tag="pgaptmp_001084"
                     /pseudo
     CDS             complement(664..2670)
                     /gene="ycjT"
                     /locus_tag="ACHOSM_RS05420"
                     /old_locus_tag="pgaptmp_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
     gene            complement(2667..3722)
                     /gene="ycjS"
                     /locus_tag="ACHOSM_RS05425"
                     /old_locus_tag="pgaptmp_001085"
     CDS             complement(2667..3722)
                     /gene="ycjS"
                     /locus_tag="ACHOSM_RS05425"
                     /old_locus_tag="pgaptmp_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4557656.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(3732..4520)
                     /gene="ycjR"
                     /locus_tag="ACHOSM_RS05430"
                     /old_locus_tag="pgaptmp_001086"
     CDS             complement(3732..4520)
                     /gene="ycjR"
                     /locus_tag="ACHOSM_RS05430"
                     /old_locus_tag="pgaptmp_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGU4557657.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(4538..5590)
                     /gene="ycjQ"
                     /locus_tag="ACHOSM_RS05435"
                     /old_locus_tag="pgaptmp_001087"
     CDS             complement(4538..5590)
                     /gene="ycjQ"
                     /locus_tag="ACHOSM_RS05435"
                     /old_locus_tag="pgaptmp_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HGU4557658.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGVTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(5621..6415)
                     /gene="ycjP"
                     /locus_tag="ACHOSM_RS05440"
                     /old_locus_tag="pgaptmp_001088"
     CDS             complement(5621..6415)
                     /gene="ycjP"
                     /locus_tag="ACHOSM_RS05440"
                     /old_locus_tag="pgaptmp_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU4557659.1"
                     /translation="MALFLIITLFPFFVMLMTSFKGAKEAISLHPTLLPQQWTLEHYV
                     DIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRLRFKGRMTINASFYTVY
                     MFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFMLKSYFDTIPDEIEEAA
                     MMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFASIFLSSASNFTLPVGLN
                     ALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAGGVKG"
     gene            complement(6450..7331)
                     /gene="ycjO"
                     /locus_tag="ACHOSM_RS05445"
                     /old_locus_tag="pgaptmp_001089"
     CDS             complement(6450..7331)
                     /gene="ycjO"
                     /locus_tag="ACHOSM_RS05445"
                     /old_locus_tag="pgaptmp_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU4557660.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(7352..8644)
                     /gene="ycjN"
                     /locus_tag="ACHOSM_RS05450"
                     /old_locus_tag="pgaptmp_001090"
     CDS             complement(7352..8644)
                     /gene="ycjN"
                     /locus_tag="ACHOSM_RS05450"
                     /old_locus_tag="pgaptmp_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4557661.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWIGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(8658..10337)
                     /gene="ycjM"
                     /locus_tag="ACHOSM_RS05455"
                     /old_locus_tag="pgaptmp_001091"
     CDS             complement(8658..10337)
                     /gene="ycjM"
                     /locus_tag="ACHOSM_RS05455"
                     /old_locus_tag="pgaptmp_001091"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HGU4557662.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLLTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(10548..10862)
                     /gene="pspE"
                     /locus_tag="ACHOSM_RS05460"
                     /old_locus_tag="pgaptmp_001092"
     CDS             complement(10548..10862)
                     /gene="pspE"
                     /locus_tag="ACHOSM_RS05460"
                     /old_locus_tag="pgaptmp_001092"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HGU4557663.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGNTHVENAGGLKDIAMPKV
                     KS"
     gene            complement(10937..11158)
                     /gene="pspD"
                     /locus_tag="ACHOSM_RS05465"
                     /old_locus_tag="pgaptmp_001093"
     CDS             complement(10937..11158)
                     /gene="pspD"
                     /locus_tag="ACHOSM_RS05465"
                     /old_locus_tag="pgaptmp_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HGU4557664.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11167..11526)
                     /gene="pspC"
                     /locus_tag="ACHOSM_RS05470"
                     /old_locus_tag="pgaptmp_001094"
     CDS             complement(11167..11526)
                     /gene="pspC"
                     /locus_tag="ACHOSM_RS05470"
                     /old_locus_tag="pgaptmp_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HGU4557665.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSETFTLRSRFRQL"
     gene            complement(11526..11750)
                     /gene="pspB"
                     /locus_tag="ACHOSM_RS05475"
                     /old_locus_tag="pgaptmp_001095"
     CDS             complement(11526..11750)
                     /gene="pspB"
                     /locus_tag="ACHOSM_RS05475"
                     /old_locus_tag="pgaptmp_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HGU4557666.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(11804..12472)
                     /gene="pspA"
                     /locus_tag="ACHOSM_RS05480"
                     /old_locus_tag="pgaptmp_001096"
     CDS             complement(11804..12472)
                     /gene="pspA"
                     /locus_tag="ACHOSM_RS05480"
                     /old_locus_tag="pgaptmp_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HGU4557667.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            12639..13616
                     /gene="pspF"
                     /locus_tag="ACHOSM_RS05485"
                     /old_locus_tag="pgaptmp_001097"
     CDS             12639..13616
                     /gene="pspF"
                     /locus_tag="ACHOSM_RS05485"
                     /old_locus_tag="pgaptmp_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HGU4557668.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHTGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(13736..15001)
                     /gene="puuE"
                     /locus_tag="ACHOSM_RS05490"
                     /old_locus_tag="pgaptmp_001098"
     CDS             complement(13736..15001)
                     /gene="puuE"
                     /locus_tag="ACHOSM_RS05490"
                     /old_locus_tag="pgaptmp_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HGU4557669.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(15039..16319)
                     /gene="puuB"
                     /locus_tag="ACHOSM_RS05495"
                     /old_locus_tag="pgaptmp_001099"
     CDS             complement(15039..16319)
                     /gene="puuB"
                     /locus_tag="ACHOSM_RS05495"
                     /old_locus_tag="pgaptmp_001099"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HGU4557670.1"
                     /translation="MTEHTRSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKNYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(16321..17808)
                     /gene="puuC"
                     /locus_tag="ACHOSM_RS05500"
                     /old_locus_tag="pgaptmp_001100"
     CDS             complement(16321..17808)
                     /gene="puuC"
                     /locus_tag="ACHOSM_RS05500"
                     /old_locus_tag="pgaptmp_001100"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HGU4557671.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(18083..18640)
                     /gene="puuR"
                     /locus_tag="ACHOSM_RS05505"
                     /old_locus_tag="pgaptmp_001101"
     CDS             complement(18083..18640)
                     /gene="puuR"
                     /locus_tag="ACHOSM_RS05505"
                     /old_locus_tag="pgaptmp_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HGU4557672.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(18667..19431)
                     /gene="puuD"
                     /locus_tag="ACHOSM_RS05510"
                     /old_locus_tag="pgaptmp_001102"
     CDS             complement(18667..19431)
                     /gene="puuD"
                     /locus_tag="ACHOSM_RS05510"
                     /old_locus_tag="pgaptmp_001102"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HGU4557673.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            19643..21061
                     /gene="puuA"
                     /locus_tag="ACHOSM_RS05515"
                     /old_locus_tag="pgaptmp_001103"
     CDS             19643..21061
                     /gene="puuA"
                     /locus_tag="ACHOSM_RS05515"
                     /old_locus_tag="pgaptmp_001103"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HGU4557674.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            21210..21374
                     /locus_tag="ACHOSM_RS05520"
                     /old_locus_tag="pgaptmp_001104"
     CDS             21210..21374
                     /locus_tag="ACHOSM_RS05520"
                     /old_locus_tag="pgaptmp_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557675.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTSPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21364..22749
                     /gene="puuP"
                     /locus_tag="ACHOSM_RS05525"
                     /old_locus_tag="pgaptmp_001105"
     CDS             21364..22749
                     /gene="puuP"
                     /locus_tag="ACHOSM_RS05525"
                     /old_locus_tag="pgaptmp_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HGU4557676.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGIGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            22883..23128
                     /gene="ymjA"
                     /locus_tag="ACHOSM_RS05530"
                     /old_locus_tag="pgaptmp_001106"
     CDS             22883..23128
                     /gene="ymjA"
                     /locus_tag="ACHOSM_RS05530"
                     /old_locus_tag="pgaptmp_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HGU4557677.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23440..25083
                     /gene="sapA"
                     /locus_tag="ACHOSM_RS05535"
                     /old_locus_tag="pgaptmp_001107"
     CDS             23440..25083
                     /gene="sapA"
                     /locus_tag="ACHOSM_RS05535"
                     /old_locus_tag="pgaptmp_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HGU4557678.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPTHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25080..26045
                     /gene="sapB"
                     /locus_tag="ACHOSM_RS05540"
                     /old_locus_tag="pgaptmp_001108"
     CDS             25080..26045
                     /gene="sapB"
                     /locus_tag="ACHOSM_RS05540"
                     /old_locus_tag="pgaptmp_001108"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HGU4557679.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26032..26922
                     /gene="sapC"
                     /locus_tag="ACHOSM_RS05545"
                     /old_locus_tag="pgaptmp_001109"
     CDS             26032..26922
                     /gene="sapC"
                     /locus_tag="ACHOSM_RS05545"
                     /old_locus_tag="pgaptmp_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HGU4557680.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            26922..27914
                     /gene="sapD"
                     /locus_tag="ACHOSM_RS05550"
                     /old_locus_tag="pgaptmp_001110"
     CDS             26922..27914
                     /gene="sapD"
                     /locus_tag="ACHOSM_RS05550"
                     /old_locus_tag="pgaptmp_001110"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HGU4557681.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            27916..28722
                     /gene="sapF"
                     /locus_tag="ACHOSM_RS05555"
                     /old_locus_tag="pgaptmp_001111"
     CDS             27916..28722
                     /gene="sapF"
                     /locus_tag="ACHOSM_RS05555"
                     /old_locus_tag="pgaptmp_001111"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HGU4557682.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHGLTKRLIAGHFGEALTADAWRKDR"
     gene            28790..29143
                     /gene="ycjD"
                     /locus_tag="ACHOSM_RS05560"
                     /old_locus_tag="pgaptmp_001112"
     CDS             28790..29143
                     /gene="ycjD"
                     /locus_tag="ACHOSM_RS05560"
                     /old_locus_tag="pgaptmp_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HGU4557683.1"
                     /translation="MMDKIKSNARDLRLNLTLQERKLWRYLRSRRFGDFKFRRQHPMG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            29511..30299
                     /gene="fabI"
                     /locus_tag="ACHOSM_RS05565"
                     /old_locus_tag="pgaptmp_001113"
     CDS             29511..30299
                     /gene="fabI"
                     /locus_tag="ACHOSM_RS05565"
                     /old_locus_tag="pgaptmp_001113"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HGU4557684.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDACIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30444..31571
                     /gene="yciW"
                     /locus_tag="ACHOSM_RS05570"
                     /old_locus_tag="pgaptmp_001114"
     CDS             30444..31571
                     /gene="yciW"
                     /locus_tag="ACHOSM_RS05570"
                     /old_locus_tag="pgaptmp_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HGU4557685.1"
                     /translation="MEQRHITVKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            31639..33573
                     /gene="rnb"
                     /locus_tag="ACHOSM_RS05575"
                     /old_locus_tag="pgaptmp_001115"
     CDS             31639..33573
                     /gene="rnb"
                     /locus_tag="ACHOSM_RS05575"
                     /old_locus_tag="pgaptmp_001115"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HGU4557686.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EIRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(33651..33755)
                     /locus_tag="ACHOSM_RS05580"
                     /old_locus_tag="pgaptmp_001116"
     CDS             complement(33651..33755)
                     /locus_tag="ACHOSM_RS05580"
                     /old_locus_tag="pgaptmp_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557687.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            33809..35793
                     /gene="pdeR"
                     /locus_tag="ACHOSM_RS05585"
                     /old_locus_tag="pgaptmp_001117"
                     /pseudo
     CDS             33809..35793
                     /gene="pdeR"
                     /locus_tag="ACHOSM_RS05585"
                     /old_locus_tag="pgaptmp_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(35775..35903)
                     /gene="ymiD"
                     /locus_tag="ACHOSM_RS05590"
                     /old_locus_tag="pgaptmp_001118"
     CDS             complement(35775..35903)
                     /gene="ymiD"
                     /locus_tag="ACHOSM_RS05590"
                     /old_locus_tag="pgaptmp_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HGU4557688.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYNAKYIYSSTYARFR"
     gene            35941..36114
                     /gene="yciZ"
                     /locus_tag="ACHOSM_RS05595"
                     /old_locus_tag="pgaptmp_001119"
     CDS             35941..36114
                     /gene="yciZ"
                     /locus_tag="ACHOSM_RS05595"
                     /old_locus_tag="pgaptmp_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HGU4557689.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36204..36953
                     /gene="yciT"
                     /locus_tag="ACHOSM_RS05600"
                     /old_locus_tag="pgaptmp_001120"
     CDS             36204..36953
                     /gene="yciT"
                     /locus_tag="ACHOSM_RS05600"
                     /old_locus_tag="pgaptmp_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HGU4557690.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37222..37440
                     /gene="osmB"
                     /locus_tag="ACHOSM_RS05605"
                     /old_locus_tag="pgaptmp_001121"
     CDS             37222..37440
                     /gene="osmB"
                     /locus_tag="ACHOSM_RS05605"
                     /old_locus_tag="pgaptmp_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HGU4557691.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(37566..37892)
                     /gene="yciH"
                     /locus_tag="ACHOSM_RS05610"
                     /old_locus_tag="pgaptmp_001122"
     CDS             complement(37566..37892)
                     /gene="yciH"
                     /locus_tag="ACHOSM_RS05610"
                     /old_locus_tag="pgaptmp_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HGU4557692.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(37892..38629)
                     /gene="pyrF"
                     /locus_tag="ACHOSM_RS05615"
                     /old_locus_tag="pgaptmp_001123"
     CDS             complement(37892..38629)
                     /gene="pyrF"
                     /locus_tag="ACHOSM_RS05615"
                     /old_locus_tag="pgaptmp_001123"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGU4557693.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINSSLQRSA"
     gene            complement(38822..39991)
                     /gene="lapB"
                     /locus_tag="ACHOSM_RS05620"
                     /old_locus_tag="pgaptmp_001124"
     CDS             complement(38822..39991)
                     /gene="lapB"
                     /locus_tag="ACHOSM_RS05620"
                     /old_locus_tag="pgaptmp_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HGU4557694.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(39998..40306)
                     /gene="lapA"
                     /locus_tag="ACHOSM_RS05625"
                     /old_locus_tag="pgaptmp_001125"
     CDS             complement(39998..40306)
                     /gene="lapA"
                     /locus_tag="ACHOSM_RS05625"
                     /old_locus_tag="pgaptmp_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HGU4557695.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVLPHSSAAKE
                     "
     gene            complement(40455..41219)
                     /gene="pgpB"
                     /locus_tag="ACHOSM_RS05630"
                     /old_locus_tag="pgaptmp_001126"
     CDS             complement(40455..41219)
                     /gene="pgpB"
                     /locus_tag="ACHOSM_RS05630"
                     /old_locus_tag="pgaptmp_001126"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HGU4557696.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            41389..41979
                     /gene="ribA"
                     /locus_tag="ACHOSM_RS05635"
                     /old_locus_tag="pgaptmp_001127"
     CDS             41389..41979
                     /gene="ribA"
                     /locus_tag="ACHOSM_RS05635"
                     /old_locus_tag="pgaptmp_001127"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HGU4557697.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(42043..44718)
                     /gene="acnA"
                     /locus_tag="ACHOSM_RS05640"
                     /old_locus_tag="pgaptmp_001128"
     CDS             complement(42043..44718)
                     /gene="acnA"
                     /locus_tag="ACHOSM_RS05640"
                     /old_locus_tag="pgaptmp_001128"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HGU4557698.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GALPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGVRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(44882..44977)
                     /gene="ymiC"
                     /locus_tag="ACHOSM_RS05645"
                     /old_locus_tag="pgaptmp_001129"
     CDS             complement(44882..44977)
                     /gene="ymiC"
                     /locus_tag="ACHOSM_RS05645"
                     /old_locus_tag="pgaptmp_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HGU4557699.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45091..45258)
                     /gene="yciX"
                     /locus_tag="ACHOSM_RS05650"
                     /old_locus_tag="pgaptmp_001130"
     CDS             complement(45091..45258)
                     /gene="yciX"
                     /locus_tag="ACHOSM_RS05650"
                     /old_locus_tag="pgaptmp_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HGU4557700.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45261..45401)
                     /gene="ymiA"
                     /locus_tag="ACHOSM_RS05655"
                     /old_locus_tag="pgaptmp_001131"
     CDS             complement(45261..45401)
                     /gene="ymiA"
                     /locus_tag="ACHOSM_RS05655"
                     /old_locus_tag="pgaptmp_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HGU4557701.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(45720..46694)
                     /gene="cysB"
                     /locus_tag="ACHOSM_RS05660"
                     /old_locus_tag="pgaptmp_001132"
     CDS             complement(45720..46694)
                     /gene="cysB"
                     /locus_tag="ACHOSM_RS05660"
                     /old_locus_tag="pgaptmp_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HGU4557702.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(46904..49501)
                     /gene="topA"
                     /locus_tag="ACHOSM_RS05665"
                     /old_locus_tag="pgaptmp_001133"
     CDS             complement(46904..49501)
                     /gene="topA"
                     /locus_tag="ACHOSM_RS05665"
                     /old_locus_tag="pgaptmp_001133"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGU4557703.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            49881..50132
                     /gene="yciN"
                     /locus_tag="ACHOSM_RS05670"
                     /old_locus_tag="pgaptmp_001134"
     CDS             49881..50132
                     /gene="yciN"
                     /locus_tag="ACHOSM_RS05670"
                     /old_locus_tag="pgaptmp_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HGU4557704.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(50168..51217)
                     /gene="sohB"
                     /locus_tag="ACHOSM_RS05675"
                     /old_locus_tag="pgaptmp_001135"
     CDS             complement(50168..51217)
                     /gene="sohB"
                     /locus_tag="ACHOSM_RS05675"
                     /old_locus_tag="pgaptmp_001135"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HGU4557705.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            51437..52195
                     /gene="yciK"
                     /locus_tag="ACHOSM_RS05680"
                     /old_locus_tag="pgaptmp_001136"
     CDS             51437..52195
                     /gene="yciK"
                     /locus_tag="ACHOSM_RS05680"
                     /old_locus_tag="pgaptmp_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HGU4557706.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHVNEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            52192..52782
                     /gene="cobO"
                     /locus_tag="ACHOSM_RS05685"
                     /old_locus_tag="pgaptmp_001137"
     CDS             52192..52782
                     /gene="cobO"
                     /locus_tag="ACHOSM_RS05685"
                     /old_locus_tag="pgaptmp_001137"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGU4557707.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(52822..53694)
                     /gene="rluB"
                     /locus_tag="ACHOSM_RS05690"
                     /old_locus_tag="pgaptmp_001138"
     CDS             complement(52822..53694)
                     /gene="rluB"
                     /locus_tag="ACHOSM_RS05690"
                     /old_locus_tag="pgaptmp_001138"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HGU4557708.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(53795..54415)
                     /gene="yciO"
                     /locus_tag="ACHOSM_RS05695"
                     /old_locus_tag="pgaptmp_001139"
     CDS             complement(53795..54415)
                     /gene="yciO"
                     /locus_tag="ACHOSM_RS05695"
                     /old_locus_tag="pgaptmp_001139"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGU4557709.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(54412..55293)
                     /gene="rnm"
                     /locus_tag="ACHOSM_RS05700"
                     /old_locus_tag="pgaptmp_001140"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(54412..55293)
                     /gene="rnm"
                     /locus_tag="ACHOSM_RS05700"
                     /old_locus_tag="pgaptmp_001140"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HGU4557710.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            55431..55475
                     /gene="trpL"
                     /locus_tag="ACHOSM_RS05705"
                     /old_locus_tag="pgaptmp_001141"
     CDS             55431..55475
                     /gene="trpL"
                     /locus_tag="ACHOSM_RS05705"
                     /old_locus_tag="pgaptmp_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HGU4557711.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    55452..55545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            55567..57129
                     /gene="trpE"
                     /locus_tag="ACHOSM_RS05710"
                     /old_locus_tag="pgaptmp_001142"
     CDS             55567..57129
                     /gene="trpE"
                     /locus_tag="ACHOSM_RS05710"
                     /old_locus_tag="pgaptmp_001142"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGU4557712.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            57129..58724
                     /gene="trpD"
                     /locus_tag="ACHOSM_RS05715"
                     /old_locus_tag="pgaptmp_001143"
     CDS             57129..58724
                     /gene="trpD"
                     /locus_tag="ACHOSM_RS05715"
                     /old_locus_tag="pgaptmp_001143"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HGU4557713.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            58725..60086
                     /gene="trpCF"
                     /locus_tag="ACHOSM_RS05720"
                     /old_locus_tag="pgaptmp_001144"
     CDS             58725..60086
                     /gene="trpCF"
                     /locus_tag="ACHOSM_RS05720"
                     /old_locus_tag="pgaptmp_001144"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HGU4557714.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            60098..61291
                     /gene="trpB"
                     /locus_tag="ACHOSM_RS05725"
                     /old_locus_tag="pgaptmp_001145"
     CDS             60098..61291
                     /gene="trpB"
                     /locus_tag="ACHOSM_RS05725"
                     /old_locus_tag="pgaptmp_001145"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGU4557715.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            61291..62097
                     /gene="trpA"
                     /locus_tag="ACHOSM_RS05730"
                     /old_locus_tag="pgaptmp_001146"
     CDS             61291..62097
                     /gene="trpA"
                     /locus_tag="ACHOSM_RS05730"
                     /old_locus_tag="pgaptmp_001146"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGU4557716.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            62478..62657
                     /gene="yciG"
                     /locus_tag="ACHOSM_RS05735"
                     /old_locus_tag="pgaptmp_001147"
     CDS             62478..62657
                     /gene="yciG"
                     /locus_tag="ACHOSM_RS05735"
                     /old_locus_tag="pgaptmp_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGU4557717.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            62743..63243
                     /gene="yciF"
                     /locus_tag="ACHOSM_RS05740"
                     /old_locus_tag="pgaptmp_001148"
     CDS             62743..63243
                     /gene="yciF"
                     /locus_tag="ACHOSM_RS05740"
                     /old_locus_tag="pgaptmp_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU4557718.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            63289..63795
                     /gene="yciE"
                     /locus_tag="ACHOSM_RS05745"
                     /old_locus_tag="pgaptmp_001149"
     CDS             63289..63795
                     /gene="yciE"
                     /locus_tag="ACHOSM_RS05745"
                     /old_locus_tag="pgaptmp_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU4557719.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(63855..>63944)
                     /locus_tag="ACHOSM_RS05750"
                     /old_locus_tag="pgaptmp_001150"
     CDS             complement(63855..>63944)
                     /locus_tag="ACHOSM_RS05750"
                     /old_locus_tag="pgaptmp_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HGU4557720.1"
                     /translation="ANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT        15359 a        16754 c        16021 g        15810 t
ORIGIN      
        1 acgggagctg gttggcgtaa tgcaatgggg ctatgccctt aatccaaaca gtggtgcacg
       61 cgtcgtaacg ggcacgcgga accgtgaata tatggagctt aaccggttat ggctgcatga
      121 ctgtatgccg agaaactccg aatcaagggc aatcagttac gcgctcaagc taatcaggca
      181 gctttatccg caggtacagt gggttcagtc gtttgcagat gagcgttgtg gctgtcttgg
      241 tgtcgtgtat caggcaagca attttgatta tgtaggcagc cacgaatcaa cattctatga
      301 acttgatggc gagtggtatc acgagatttg caggaatgcc attaaacgag gcggacagcg
      361 aggtgaacat ctgagggcta atatcgacag agccagtgtt cataaattcc gtcagtttcg
      421 ttacatccgg tttctgaaca agagggccag aaaacgcctc aacacaaagc tattcaaagt
      481 ccagccttac ccaaaaccac aaacagttaa aactggttta aaagagagtg agtgaacgct
      541 taaaaactca ttctaaaacc caaaagaaaa gcgccgattt agtatcaaat aagttgcgta
      601 tcggcgtttt tcagcggtat caaatgaaat gcaaatcggt atcaaaaaaa tcgccgcgct
      661 atacccggca aaaccctgaa tcgcccccag ccagatagcg ccagttgcgg cagcatggat
      721 accatcatca caactatgcg gatcagcacc aagatcgatt tcagtcccct cgcgccagaa
      781 ctgataactt tgggtcagca ggccacagcg tgcggcaaca atgccgtgga ttgctttact
      841 taatgacgag tcgtgaatag tgcgcggttc ataaaattgc agattggcaa gacacgatgc
      901 cgctgagaac tgctccggca gcatgtaatt gagcatcacc acatcagctt gtttgaggat
      961 ttgcatctcg ttcacttctg cgcgtgaata atccagcaga atggtttgct tccccgccgc
     1021 cgctttgtat ttcgccagat taatcaccgg cttagccata aacgaatcat cctgcggcaa
     1081 aacgccgtcg ggctgaattt ctggcagccg cagttcttta aggaacattt cggcgcgatg
     1141 gataaacgca tcgtcgctac agccaaactg gcgggcaata ctcagcgcct gctggacgtt
     1201 gtaatacgcc atatagctgg tgaaggcgtt attattgaca tgttcggtat attcgtcagg
     1261 cccaataaca tcatgaattt ccagacggtc gttaaccctc accgcgcggc taatccagaa
     1321 ctttgcagtt tccagaagta gcgccatgcc ttcatgcgca atgaaacttt catcccccgt
     1381 ggtctgccag tattgaataa ccgcccaggc gatatcggcc accagatgat gttccgcctg
     1441 cgccgaggcc actttttgcc gcagcccggt gcgaatgtta atggcggcaa attccggcgt
     1501 ctcttcttcg ccgctgcgcg cgctttccca cggaaatagc gcgccctgcc agccgttgcg
     1561 tcgcgctttc tcctgcgcgc ctggcaagtt gtgccagcga taacgcagta aacttcgggc
     1621 aaccgtcgga tcgctaaaca gatgaaacgg taacaaaaat acttctgtat cccagaaaac
     1681 gtggcctttg tagccttcgc cggttaagcc ttttgccgca atgctgctgc gctcgtcgtg
     1741 ggcaggcgtc atgatgcgca gatgataaag cgcataatct aacgcttgct gatcgtgcgc
     1801 ttcgccgcca tttaccgtga tacgacgttt ctgccaccat tgacgccagt tttctgttga
     1861 tactgcaaga agttggtcgt aactctgctg cgcgcacatt tcaagctggc gaagcgacgc
     1921 gctgccccac tcgtctaaaa cggcttgcct gtcatcccgc cagtcgatcc acaccagttt
     1981 ttgcaacgtc actgtctcgc ctgcatgaag ctgcgcactg gtatgttgct gcaaacggcg
     2041 ctctttggcg gtatagcatt gctgcacatc accgctcacc atacagcaac agctgatggc
     2101 cacatcactg cgtccattct gggtggtgta gatcccctgc atcagatgct gaccaaacac
     2161 ccgcacctgg gtttcgtcga gatgttggcg accgtggttg gtttgcgtgg cgtcgatacc
     2221 tgttgaaatc agcactgacg cgtcggcgtc cagtggcgta atagtgattt ccagcacaat
     2281 gagcggcagt tggtctgccg aaacaaagcg acggctggcg atggtgtaac ctgtgccgtt
     2341 gctggtacgc cagacaacgc tacggcgtaa ttctccactg gcaaagtcaa gttcacgctg
     2401 ccaggcttcg cgggataacg agaaaacctc accatttatg gcaatctcca tccccacgac
     2461 atcaggcagg ttcaccagtt cgttgatttc accttttccc gcccgatgat acagccccgc
     2521 cagatacatc cctcgcgtct ggcgcgtgta atcttcttca tggctggcgc gaagcccaag
     2581 atagccgttc ccctgcgcca tcagcgatgc atacttattc agggtatgct ggctgaaatg
     2641 gggttctgat aacgttactg gcctggtcat aattccacac gcgtccctgt ttctgcggat
     2701 tgatacaacg ccgccaccag ttgctggatg atgtacccct gctcggcgtc ggctatcatc
     2761 acgggcttgc cctgtacgtg attgataaag gcttccatgc tgcgcaaatg gcggttgtcg
     2821 tctgccagtt cccgttgcat cagcgtcatt aattcaccgt tgttatcggt gtagatatgt
     2881 gctggaaaca gcgtcgcacc ggctttatca ccacaaaagc tgacgttcat aatcgactgt
     2941 tcgcggatgt tgagtgcaaa tgacgtttcc agccacagaa tgccgccgtt atgaaattca
     3001 atggtgccaa acagcgaatc ttcgacgctg taagttgccg gatcccactc gccaaattga
     3061 ccacagctct tttgcgtgcc gatcttttga aagctatgcg cattcacgct tttcaccgcc
     3121 ggaaagccca gcacatacat cgcagcatcc agcatatgaa tgccgatgtc gatcagcggg
     3181 ccaccaccct gcagttcttt attggtaaag acaccccagc cgggaacgcc gcagcgacgc
     3241 agggcgcggg cggtggtaac gtaaatctct cccaaaacgc cgttggtcac ctgttcacgc
     3301 agctgttgcg tatcgagcgc aaaacgatgg tgaaagtcgt aggccagcac cttgcccagt
     3361 ttgcgcgcgg tatcgcacat ttcccgcgcc tgttctggcg tcatggcggg cggtttttcg
     3421 cacatcacat ggcagcccgc ttccagtgcc atcagggtat gttcgtaatg aaaacggtta
     3481 ggtgagcaga cgctaaccac atcaggtttc accgccagca gcatggcctg cggatcgtcc
     3541 cacacggatg cattaccgta tttatctgcc agcgcctgcg cctgggaaag gcggctgtca
     3601 cagacagcca ccagttccag atcgttgcga gtgcagtagt acgaagcatg aaccttatcc
     3661 gccacctggc ctgcgcctat tatcgcgacc cgcagcggag agcttgtcat tgcacttttc
     3721 acaaagacct cttagcaggt acgcaaccag gccaacgaat cacggtacgc ctgggcagga
     3781 tcttccgccc ggatacgccc ttcgtacact acatagccct gatagttatc cgcgcgcagc
     3841 tgttcaaaca gcgcgtggaa atccagggtg ccgctgcccg gctggtagcg atggttatcc
     3901 gcaatatgca catgaccgag caggtcgcgg ttgtcatgca gcgcctgcgc caggttatct
     3961 tcttcgatat tcatgtgata gaaatcgccg ataatctgta catgtttaag atcgttttcg
     4021 acgatgtaac ggcgggcatc ggcgagggtg ttgatcatat gatcctgata gcggtttaac
     4081 ggttcgagat acaccacggt tccggtacgc gcggcgacct gttccagtac gcgcagggaa
     4141 tcactcacca ttttgcggtc gccgtccagg ctacgcggcg aggtcatcgg cggtaagcgg
     4201 aaggtaaaca tgccccacgc tgccggaacg acgatacctt taccccccac ttctgccagc
     4261 gcttcgagaa tgcgttcgat ctgctttaag ccattaagac gacgctcttc gataaagtcg
     4321 ccaatccaac cgtcatagcc accgcaggcg gtggtcaccg gtaaaccggt ttctttgatt
     4381 gctgctttga cttcttcgag attgttgacc agcagtttgc cgtcaatctc aaaaccatcg
     4441 aagcccatct ctttgatata acgaaatttc tccagaatgt tttccggaaa gaatgcctga
     4501 ttctgtgtgc ctattttcat tttttcattc ctttgaatta aaacgtaacg cccattttga
     4561 tgctctgttc cggatgctga tcgacatact gcatgtagct ttccgggcta ttggcaaagg
     4621 tcactaccgg gtcgattaaa tcttcgcaat tgagataacc gttcatcaac agttcccagc
     4681 aggtttcttc aatacgctta cggctccagc gcggataatc cgggttcggt tcgctgcacg
     4741 cgcgggagaa aacaattttg gcgttattga aatgcgcttc gcgtccgagg ttaaaacctt
     4801 cagcaaatgg tttggcaaac gcgacatagg agatggtgac accgtaggcc agaccgcgga
     4861 acgccgattg cagcgcgtcg gcatagccgc tggtttcgat aatcacatcg gcaccctgct
     4921 tgccggtcag cgttttgatc tctttgccta cgtcagtacc aatggggtta aggcagaaat
     4981 ccgcgccgtg gcgacgggca atatcacagc gatgagcgat aggatcgacg ccaatcacga
     5041 ccgacgcgcc agcacgttta gccagttgaa tggcaatttg accaatcgcg ccaagcccta
     5101 ccaccacaac aaaatcaccc acgcgcacgt tggcatcgcg cacgccgctc atggcaaact
     5161 gcgccgggtc gtaacaaacg gcgtttttcc acgagctgcc ttccggcatt ttgcgcagct
     5221 tgtagttatt caccgcgtta atgatgaccg tctcggagag cgggccgtag ccgcatacgc
     5281 tgtcgcccac cgcgtagtcg gtaacgtcgc tgccgcactc gataatgtcg ccaaccacca
     5341 tgtttccaag ctggaatttg ccaaactcaa tgccgcgcgg cgcatctgcc ggacgcggag
     5401 tgaacatctg ccattcgccg ttaaagtctt catcgataaa cgggctggcg gcgcggaagt
     5461 cgaccacttc cgttccgtgt ttcggtgcgc cgaaacgcac gcggaccttc acttcattgg
     5521 ctaaaatcgc ccgatcttca tactcaacca gcgcagcaac acgcggtgct gtggctacta
     5581 actttttcat ttaaaactcc ttgtaaagaa actggccgct ttagcccttc acgccaccgg
     5641 cggtcaaacc actcttaata aaacgttccg aaagcgcata cataatcacc accggcaatg
     5701 cggttaccag tgaggccgcc atcatccgtc cccagatgta atctggcgta ctgaacagcg
     5761 cgttcaggcc caccggtaag gtgaaattgc tggcgctgga gaggaaaatc gacgcaaaca
     5821 gatagtcgtt ccacgccacc ataaagcagt agacgaacac ggatatcagc ccggacatcg
     5881 ccaacggcac ggtgatgcgg aagataattt gcagacggtt gaggccatcc atcatcgccg
     5941 cttcttcgat ctcatcaggg atggtgtcga agtagctttt cagcataaac acggcggtgg
     6001 gcagagtttg cgtcaccatg gtgatgatca gcgccatctc ggtgtcgtaa atgcccagcg
     6061 cggtgatgat tttgaacagc ggcaccacca gcaaaatgcc agagaacata tacaccgtgt
     6121 aaaagctggc attgatggtc atccgacctt taaagcgcag gcgggaaagc gcgtaagccc
     6181 caagaatgcc gaggaatacc gccaccacgg atgaaaccac agacaccacc agactgttac
     6241 ggaagtagtc gacaaacggg aaaatcatcg ggttaaaaat gtcgacgtaa tgctccagcg
     6301 tccactgctg cggcagcagc gtaggatgca gtgagatcgc ctctttcgcg cccttgaacg
     6361 aggtcatcag catcacaaaa aatggaaaca gcgtgatgat gagaaacagc gccagcccgc
     6421 actaaaaacc gatgcgactg agtgtgcgtt tatttgttgc cattgaggtt cacccgtttc
     6481 ctggtcagca gaatgactgc gaaaataatg atgaagagca ccaccgagat cgccgccgct
     6541 tttcctaaat cattaaaggc aaaggcggtt ttgtagagat atacaccgag aatatcgacc
     6601 ttggtcgtca gcaaataaac atccgcgaac atgtagaaca tccagatggt gcgcagggtc
     6661 accaccgtcg ccaggaccgg cataatcgcg ggcagcgtga cgatacgaaa ccgttgccag
     6721 gcattagcgc catccatctc tgccgcttcg tacagcgatt tgtcgatggt ctgcaaaatc
     6781 gccagaaacg agataaaggc atacgggaag tagcgccaga tggcgaacag caccaccagc
     6841 acaaagctac tgcccggatt atcgaaccac agtggtgcct gctcatagag atgcagaaga
     6901 tcgacgccga ggtagttcac aatgccgtag ccgttgttga acatgtattt ccaggcaaac
     6961 accagcgaaa tggacggcgt tacgtaggag aggatcacca gcgaacgcgc ggttttgcgc
     7021 aggcggaact cgcggttgaa aaacatcgcc actgccagcc cgagcacggt gctgcccgcc
     7081 accaccagcg cggtatacca gaccgtcatc cacagcgagt gccagaagcc gggatcggag
     7141 aggatacgca catagttact caccccaaca aaggttgact cgatgttggg attgagcggc
     7201 agacgtaaaa aactgatttc gatattcgac accattggcc aggccaccag accacccagc
     7261 agtaataagc tgggcgcgag aagcagcagc gcaaagggca tatcggaacg acctgaaaaa
     7321 agcctgttca ttgcggattc ctgtcctggg tttagtgctg ttcgatcagt tcatccagct
     7381 ttttctggct cgcttgcagc gtgccaggga gatcggcttt acccacggtg acgttatgca
     7441 ccatggagct caccacgcca gaacccgtca catcacccat gcgggtaaag tttttatccc
     7501 ctactgcgcc aaagacctga atatttggca gttcaccgat tagctgattc ggtagttcac
     7561 ccagcgcctt aataacgtcg ttgtctttcc aggtggcggt agtgacaacc gctttattca
     7621 ccggcagcgc agcacctggc gacatcatca cccagtcggc aatgttgtct gcctgctcca
     7681 taaaggtgac aaatttttct gctgcttccg tctcttcggt cttttgcccg gcggtaatgg
     7741 tcagcgaggt caacatgccg tagaccgcag agtttttctc ggttggcacc acgaaaccga
     7801 cgtttttcgg gtcgccttct ttaatcacag ccggaaggat ataggtggag taaatcgcca
     7861 tcggcgcggt gccgttcata aaggcgtctt tcacttccat gatgtcgtta gaacccggca
     7921 tagtgttggc agcaaggtcg cggtaatagg tcagtgcctg catcatctct ggtgtatcaa
     7981 gggtgatttt gccttcggcg ttaaagacgt tagcctggtt ggataacgca aactgggaga
     8041 aggattgttc cgtcaacacg ctttctgctg taggcagcgc aatgccgtat tttttattcg
     8101 ccgggtcatt cagtttctgt gcaacgtcca gcagctgttg ccagtttttc ggctcctcaa
     8161 gccccgcttt tgccagcaca tctttgcgat accagatacc gccaatccag gcgctgacag
     8221 gaacaccgat ccatgcgcta ccatcttcgg tacgcacaat acgcagtacg ccatcgtaaa
     8281 acgcgccttc accaacgttg ctgatgactg tggcaaccgc tttgcgatcg ataagctgct
     8341 ctttgtccat cactttggcg tagtcatggc tggtttctat cacttccggc agcgaaccgc
     8401 tacgtgaaag agtaatgact ttagtgttat aggcatcttc ttccacgggc acttgcttaa
     8461 cggtgatgcc agggttttct ttttcaaaac gggcaatcaa tttactgata acggcctggc
     8521 gctcctgctc caccgaagaa tgcataaatt cgatggatac attcgtttta ttttcttctt
     8581 tacagcctga aatcagggcg caggaaacca gtgctgataa cagcacgatt tttgatttaa
     8641 tcatgagatg ttccttttta tttaatccac ataacctgcc aggggcgcag cgttatttca
     8701 ttacccaata tatcaacttc actaatcaga tcccgaccat gcagattagt aatatttaca
     8761 tgctgaatat ttttactgac attaaacaat ccagtcagac aattaccatc agcgttactt
     8821 ctttgaatac gcattacgga tgaattaatc gtatcgatgg taaaattatt atccggatga
     8881 aactcgttat ggctgcgacg aagtgtaatt aaacgcgaca actcatgata taccgcatgc
     8941 cttaatgtag cttcatcgtt cagttctcgg gttatctctt tactgtgata ttttttacgg
     9001 ttaatcgcac ggttatatcc gagtttttcg acacctgcgt aatcattacg cgagccaaga
     9061 atactttgaa tatatatcgc tggcacaccg gggaaactta acaaaatcgc atgggcgaga
     9121 ataaacctgg cgcaacgttc ttcatcgcta ctctcacggc ggcttaacgc atccatatag
     9181 gtcacattta tttcatacgg actgcgtgta ccgtcgggat tatttttcca gtttactaat
     9241 gcaccttcct gctgtaacgc ctcgaccagc tctaatattt cgctttcagg caacaagccc
     9301 cgtagcgggt ttagcccgat gccatcgtga gaggcgagga agttaaacca ggtggtgttg
     9361 ctggaaggta gtgtcaggtt ttgcgcccac gcacaaagcg cctcaacgtt ctgtttttgc
     9421 accgcatgca gcaccagtgg cggcagcgag aactggtaca ccatatgtgc ttcgtcatcg
     9481 cctgcgccaa agtaagcaat gttgtcttta tgcggaacat tggtctcggt aatgatcact
     9541 gtacctggcg caacgttatc aataatcgac cgtaacagtt tgataatcag atgtgttttt
     9601 tccagatgga tgcagcttgt tcccggctct ttccacataa agccaacggc atccaggcgg
     9661 acatactcag cacctttcgc aaggtaacag agtaaaacat ccaccatcgc cagcaacact
     9721 tcagggctac ggtaattcag gtcaatttga tcgtcactaa aggtggtcca taaatggcgc
     9781 gttgaatgat cgcgcatctg gaatggcgtt aataacggta acgcacgcgg gcgagtgacg
     9841 gcgctgagat cggtttgcgg gtcaacggca ataaaaaaat cttcaaaacc tggatgctgt
     9901 tgtaaatagt ttttaaacca ttcacttttt gccgacatat ggttgcagac aaaatcaaac
     9961 attaaatgac tgcattcacc gagttgctga atatcctgcc actcccccgc ttcactggcg
    10021 acctgatgat aatcaattac cgaaaagcca tcatcagatg accatggata aaacggcaac
    10081 aaatgaacat gtgaaaaaat gctttgcagc cattgatggt aaaactgatt aaatgtgagt
    10141 aatggtttta aatcattgct gtgaaattga tcggcatagg taattaacac aacatcactt
    10201 tcatcccagt gttttttacg tcgctgtgta attaatcgtt tcgcactctc aagacgcgtt
    10261 acaagtttct gtaaatgagt tgcggtaaat gttccaccgt agatttcgtc caggtaatcc
    10321 gtaattttct gtttcatgtt gcctttccgt ggtagcggtc ccatgaaacg agagactact
    10381 ccgcagaaaa taaactgtca attgagcaca agggttttag tcacgcagga aatcattttt
    10441 taccagggaa aaagcgtatt tgtgagctga cgcaaataat ctatggggat gtaaataagg
    10501 tgtgacagaa aaaaacggcg cataagcgcc gcacatggtg aattctttta acttttgacc
    10561 ttcggcattg cgatgtcttt caggccaccg gcgttctcaa cgtgggtatt tcccatctcg
    10621 ctaaggatct cttttgcctg ccctgactgg cgtccggcat tgcaatacac tttcacggtg
    10681 tcgtttttat ccggaacggc ggtggcaatg cgctctttca cttccttcag gggaatattg
    10741 atggccccct gaacgtgctc ttgctgatac tgctctggaa cacgaacatc gatccagtgt
    10801 tcagcggcga aaacgggcag tgaaaacacc agtgccagag caagtaagcc ttttttaaac
    10861 atgtaagcgt ccttctatgg atgaaaattg ccatccatta tacgcctgag atttaacaat
    10921 ttttgcgaaa ctcccctcac cttttataac gctgtgccag tttattggca gcccgactta
    10981 acagcggttc cagcgccaca gccagcaaca ttttcagcgg tcggcgagca actgatttta
    11041 tcgcccagcc cgccacaccc gctgggccat agcgcagtgc ggtaagaagt accagcttgc
    11101 ctgctaattt gaaaccaggc tttacctttt gcccggcctg ttgccagcga gtattcataa
    11161 ctttcctcac agttgacgga aacggctacg taacgtgaat gtctcggaag tgacataacg
    11221 ttccatctcg cgtaaacgcg tttcacttgc cgccagttca cggtcgactt catccagcaa
    11281 ttcactgctg gaaggaagct gctcaccaaa ggccatgttg tccggcattg gatcaagcgc
    11341 aaatgacaaa atgatgtaag caaccagggt aaacagcgcc agaccgaaga aaatcgacag
    11401 caccaccagg atacgcacca gttttactgg tacatcaaaa tagttggcaa tcccggcgca
    11461 gacgccgcgt accataccct gctgtggaat acgccataat tttttattga gattaatgcc
    11521 cgccattagc gatccctcca gttcggatgt tctgcatcaa gaatagattc cagcgcctga
    11581 atacgttcgc gcatccgttt tgcttcatca gccagttgcg ctaatcgctg ctgctcactt
    11641 tgcgacaatt cactgcgacc agaacgattg ctgtaatgca gccataacca gatcggtaaa
    11701 acaaacagca caaaaatggt taacggaata gccagaaata gcgcgctcat aagtactcct
    11761 tagacgatga gcggcggcgc gttcggacgc cgcctggatg ttattattga ttgtcttgct
    11821 tcattttggc ttttaattgt gccagttgtt cgctgattgc atcatcggct ttcagttcgg
    11881 caaactgatc gtccagcgat ttttgtttac cgaagctgtg gctttctgct tccgcttcca
    11941 tctggtcaat acgacgttcg aaagattcga aacgagccat tgcttcatcc agtttgccac
    12001 tgtccagctg acgacgcaca tcgcgcgacg agtttgccgc ctgatgacgt aacatcaatg
    12061 cctgctggcg agcgcgtgtt tcgctcaatt tgttttccag ctcgccaatc tctttcttca
    12121 tgcgtgccag cgtatcgtcc accagcgtca cttcatgttc cagggactta atcagatcgg
    12181 tcagtttctg tttttcaatt aacgctgcac gtgccagatc ctctctctct ttcagcagcg
    12241 ccagttcggc tttttcctgc cattcaactt cacgcgccga cgcttgttca atacggcgag
    12301 tcagctgttt cttttctgcc aacgcgcgcg ccgaagtaga acgtacttca accagtgtat
    12361 cttccatctc ctggatcatc agacgaacca gtttctgtgg atcttccgct ttctctaaca
    12421 gagcgttgat gttggcattc acgatgtcgg caaagcgaga aaaaataccc ataatgttgt
    12481 cctcttgatt tctgcgttca ggattgtcct gctgaactgt taatacaatt tgcgtgccaa
    12541 ttttttatct ttttgattta taaagatctg attgaagaat caacagcaac atgccaggat
    12601 gagttagcga attacactaa caagtggcga atttcatcat ggcagaatac aaagataatt
    12661 tacttggtga ggcgaacagc tttctcgaag tgctggaaca ggtttcgcat ctcgcaccgc
    12721 tggacaaacc ggtgctcatc atcggcgaac gcggcaccgg taaagagctg attgccagcc
    12781 gcctgcatta tctctcctcc cgttggcaag ggccgtttat ttcccttaac tgcgcggcgt
    12841 taaatgaaaa tctgctggat tccgaactgt ttggtcacga agcgggggcg tttaccggtg
    12901 cgcaaaaacg tcatacaggg agatttgaac gtgccgacgg cggtacgcta tttcttgatg
    12961 aactcgctac ggcaccgatg atggtgcagg agaaattatt gcgcgtgatt gagtacggtg
    13021 aactggagcg cgttggcggc agccagccat tgcaggtgaa tgtgcggttg gtatgcgcga
    13081 cgaatgccga tctcccggcg atggtcaatg aaggcacttt tcgcgctgac ctgctcgacc
    13141 gactggcttt tgatgttgta caactgccac cactgcgcga gcgcgaaagc gacataatgt
    13201 tgatggcaga acactttgcc atccagatgt gtcgggaaat caagctgcct ctgttcccgg
    13261 ggtttacgga gcgcgccaga gaaacattgc tgaattatcg ttggccggga aatattcgtg
    13321 aattgaaaaa cgtggtggaa cgttcagtgt atcgccacgg caccagcgat tatccgcttg
    13381 atgacatcat tattgatccc tttaaacggc gtccgcctga agacgctatc gccgtttcag
    13441 aaaccacctc gcttccaaca ctgccgctgg atttacgtga gtttcagatg cagcaggaaa
    13501 aagagttgct gcaactcagt ttgcaacaag ggaaatataa ccagaagcgc gcggctgaat
    13561 tactggggtt aacttatcat cagttccgcg cgttgttgaa aaagcaccag atttagcgaa
    13621 gttgagcaat acaacttgcc ggatgcggcg tgaatgcttt attcggcata cataatcaat
    13681 acgtcgtttg caggcctgat aaacgcagcg catcagacat tattgcgttg ggctattaat
    13741 cgctcagcgc atcctgcaaa attttcattg ccgcatcgaa ttgcgcatcc gggatggtca
    13801 gcggatacag gaagcgaatc acgttgccgt atgcgccaca ggtcagcagg agcagcccct
    13861 gcgccagcgc gcgttgctgg attttctgtg caatcgccgc tgacggctcg cccgtttgcg
    13921 gatcgttaaa ctctgccgca atcatcgacc ccaggccgcg taccgcagca atggccggaa
    13981 cgctttcttt ggcatcaatc aacgtgtttt tgagacgctg gcccagttga ttcgcgcgtt
    14041 cgcagagtga ttctttgtcg ataatattga gcaccgcgtg cgcggcagcc accgccagcg
    14101 ggttcccggc gtaggtgcca cccaacccgc ccggcgcggg cgcgtccata atattcgcgt
    14161 taccgaccac gcccgaaagc ggcatcccgc ccgcgaggct tttcgccatc gtcattaagt
    14221 ccggcttatc ggcgtaatga tccatggcaa acagtttacc ggtacgcgca aagccgcttt
    14281 gcacttcatc ggcaatcatc acaataccgt gctcgtcgca caggcggcga atggcggcaa
    14341 ccagctcttt tggcgcaacg ttgaaaccgc cctcgccctg caccggttcg aaaataatgg
    14401 ccgcgacctg cttcgcttcg atgtctgatt taaacaagcg ttcgatggcg tcgagggagt
    14461 cctgtgttga aatgccgtgt aaatctgacg gataaggtac gtgatacacc gaaccaggga
    14521 acgggccgaa gccgattttg tacggcgcaa cttttccggt cagcgccatg gtcatatatg
    14581 tacgaccgtg gaagccaccg ctaaacgcaa tcacgccagg tcgtccggta tgggcgcgag
    14641 caattttcac cgcgttttcc accgcttccg cgccggtagt gaagaatgca gttttagcct
    14701 gcccgctcac tggggcaagg gcgttaattt tctccgccag cgtgacgtag ctttcgtacg
    14761 gcacaatctg atacgccgtg tgggtaaact gttgcaattg ctgctccacc gccgcgacca
    14821 gatcagggtg gcgatgcccg gtattcagca ccgcaatgcc tgcggcgaaa tcgatgtatt
    14881 cgttgccctc tacgtccttc agcgtagcgt tttctgccga ctgggcgaag aagttacaca
    14941 tcacgccaac cccgcgtggg gtagcagaaa gacgacgctg atggaattca ttgttgctca
    15001 tgtttcacac cttttgactc ttcactgtta atcggaaatt aaacgcccag acgatcgcgc
    15061 aggctgtaat aagccgcgcc catcgcggta aatggcacgc gcagcgtgcg cccgccgggg
    15121 aacgggtaat gcggcagatt ggcgaaggca tcgaaacgtt cggcgtcgcc gcgcagcagt
    15181 tcggcaatca gacgcccggc aagatgggta caggtgacgc cgtggccgct gtagccctgc
    15241 atgtaataga tgttggtatc aaggcgaccg aactgcggca tgcgcgacaa ggtcagcagg
    15301 aagttgcccg tccagcggta atcaattttc acgcccttca gctgcgggaa ggttttcagc
    15361 agtttcggca ccacaaggcg ctcaacgtca tccgggtcgc gcgcgccgta gaccacgccg
    15421 ccgccgtaca gcaggcggtt gtcggcggta agacggtagt aatcaagcag atagttacag
    15481 tcttccacac agtagttttt cgggatcagc gaacgggcta aatcttccga cagccgttcg
    15541 gtggtgatca cctgcgtgcc gcacggcatg ctgcgtttcg ccagttccgg ctctacttta
    15601 tcgcccagat acgcattccc ggcgacaatc acatacttcg ccgtcacctg acctttggca
    15661 gttcgcacaa cggctggcgt ggtgtgctgg atctgcgtca cggcagaaag ttcatacacg
    15721 cgcccgccgt tgaggcggat ggcgtccgct tcgccaatgg cgaggtttag cggatgaata
    15781 tgcccaccgc tgtgatccag cagcgcacca gtgtagcgat cgctggcgac ctcgcggcga
    15841 atagcgttgg cgtccagcag ctccagctgt ttattgccgt agcgttccca gttctctttc
    15901 tgctcttcga gtgtggcgag ttgtttatca ttcatcgcca caaacagacc gccgggacga
    15961 tagtcgcagt caatctgata acgtttgata cgttcgcgga tgatctcgcc accctcgaac
    16021 atcatgctgc cgagcatacg ggcggtgtcc atgccgtagt ttttttcgat cacgtcgatg
    16081 tcgcggctgt aggagttcac aagctgcccg ccattgcgtc cgcttgcgcc gaagccgatg
    16141 cgtgaggctt caagaaccac cacgtcaaag cccgcttccg ccagatgcag cgcggaagag
    16201 agcccggtat agccgccgcc gaccacgcaa acgtcgcagg aaatcgactc attcagcgtg
    16261 tcgaatggcg cgtatttatt cgcactggcg gcgtagtaac tgcgggtatg ttcggtcatt
    16321 tcaggcctcc aggcttatcc agatggtttt cagttcggtg aatttttcaa gggcatgcag
    16381 ggatttgtct cgaccgttgc cgctctgctt atagccgcca aacggcacgg tcatatcgcc
    16441 gtcgttgtag ttattgacga agacggaacc ggctttcagg cgacggctca tgcggtgggc
    16501 gcgggagagg tcgcgcgtcc ataccgccgc gccaaggccg tactggctgt cgttggcaag
    16561 ctgtagcgcc tggtcttctg atgtgaaacg cgtgaccacc agcaccggac cgaaaatctc
    16621 ttcgcgactt aaggacgcat tcgggtccac atccacaaag atggtcgggc cgatggcggc
    16681 agccagctca gcgttacgac catccagcag cagttgccct ttgctttcgc cttcccgaat
    16741 aaagctatgg accgagtcgg cgtgggcgca gtcgattaag gtgcccatgg tggttgcggg
    16801 atcaagtgga tggcccggct gccagttttg cgcctgctgt tttaacaggg ctaagaattc
    16861 atcggcgatg ctctcttcca gcaacaaacg cgttccggcg atgcacacct ggccctggtt
    16921 gtagaagatg cctgcggcag tggcgcttgc cgctttttgc agatccggac aatcggcgaa
    16981 aacgatgttg gcgcttttgc cgcccgcttc cagccagacg cgtttcatgt tgctgtcgcc
    17041 cgcatctttc agcagctgtt tcccggtacg ggttgaaccg gtaaaggcaa tggcgtcgat
    17101 atcgttatga cgcgacagcg cctgccccgc ttcatgacca aaacccgtca ccacgttcaa
    17161 cacaccatcc ggcaagcctg cttctttcgc cagccccgcg agacgaatcg cactgagcgg
    17221 tgatttttca gacggtttca ggacaacgct gttccctgct gccagcgccg ggccaagctt
    17281 ccagcaggtc agcagcagcg ggaagttcca cggcacgatg gcggcaatca cgccgaccgg
    17341 ttcacgcacg atcatcgcca gctcatggct actggtggtc gccacttcgc catacacttt
    17401 gtcgatcgct tcggcgtacc agcgaatggc gcgcgccgcg ccgggaatat catcacgcag
    17461 actgtgacga atcggtttgc cggtgtcgag agtttccagc agtgccagct cttcggcgtg
    17521 ggcttccatt aaatcggcga gtttattcag taccgcttta cgtttagccg gagaagagag
    17581 tgaccagtcg ccgcgttcaa atacgccgcg tgctgcgctc accgcacggt cgatatcgac
    17641 gctcttgccg cgggcaattt tcgccagcgg tgcctgggtg accggatcaa cggtttcaaa
    17701 ggtttcattt tccgccgcag cagtatattc accgttaata aataagcggt tttcaatggc
    17761 gagacttaac gctttatcct gccagtaagc cagatgatga aaattcatta tgactcctgt
    17821 ttcacgtcta tcagatatat gcagtttgtg aacgtcaggg cgcagagatc cccgggccgt
    17881 atgcccgttt atttctggcg acattcttta tatacacttc atccttcaag ccgcctctgc
    17941 cttggctgcg ttcgctcccc cgaatcactt acctgagtaa gctcatcggg attcgctcac
    18001 ttgccgcctt gatgcaactt gaatgatttt gtgtatagat tgccgggcgc attgcactta
    18061 cccggcatac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
    18121 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
    18181 gccccgcgac gagatgataa tcctgaccat taatcgtcag aacaatttca ccttccagta
    18241 cagtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
    18301 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
    18361 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
    18421 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
    18481 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
    18541 gagtcagccc ggagagttca gcggcacgac gttgtgaaag cccctgctgc tggcggattt
    18601 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
    18661 tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga taaagccctc
    18721 gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc actgtacgcc
    18781 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
    18841 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
    18901 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
    18961 atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat gttccagcaa
    19021 ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca ccagttcttg
    19081 taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat taattatcgc
    19141 catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt caccatatag
    19201 gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt tcggcaaaag
    19261 ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca agccgcctgc
    19321 gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac ccttaagcct
    19381 gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca tgctcaatct
    19441 cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat gttcaatatt
    19501 ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt gacaaacatt
    19561 tagtttgcat acagattcga atggtggtca ttatatttta cactttgata acgagcggaa
    19621 aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga actttgttca
    19681 gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct acctggagcg
    19741 ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt gcttccgtgg
    19801 aaaacgtatc cctgtttcaa gcctgaagaa gttggagaaa gggtgttatt tcccggcctc
    19861 ggtgtttgca atggatattc tgggcaacgt agtagaagaa gcgggtctgg gtcaggaaat
    19921 gggcgagccg gatcgtactt gtgttcctgt tctcggttcc ttaacccctt ctgccgccga
    19981 tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg ctccctttga
    20041 cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctacgccagc gcggattgtt
    20101 cccggttgta gcggtagagc tggagttcta tttactggat cgtcagcgcg acgctgaagg
    20161 gtatctgcaa ccgccctgcg cgcccggcac cgatgaccgc aatacacaaa gccaggttta
    20221 ctccgttgat aacctcaacc acttcgccga cgtgctcaat gatattgatg aactggcgca
    20281 gttgcagctg attccggcag atggcgcggt cgctgaggcc tcgccgggtc agtttgaaat
    20341 caacctttac cataccgata acgtgctgga agcctgcgat gatgcgctgg cgctaaaacg
    20401 cctggtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg cgaagccgta
    20461 tgaagagcac gcgggcagcg ggatgcatat ccatatcagt atgcaaaata atcgtggcga
    20521 gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg ctgaaaaaga tgctcgccgg
    20581 gatgattgac ctgatgccgt cgtcgatggc gttgctggca ccaaacgtga actcgtatcg
    20641 ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata acaaccgcac
    20701 cgtcgccctg cgtattccgt gcggcgaccg tcataatcac cgcgtggaat atcgcgtggc
    20761 gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta ttttgcatgg
    20821 ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg aacaggaagg
    20881 cttacccttc ccgattcgcc agagcgatgc cctgggtgag tttatcgaga atgatcacct
    20941 gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa atgatgagct
    21001 gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga aaaacgcgta
    21061 attccccatg cggggttgtt tcatcggcct gagcggagcc aggcctgaca tttgctggta
    21121 atccagacat ttcccggatg tcgcgttatg gagcgcaggc ggcaacgggc ttccgttaac
    21181 gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg catggcgagg
    21241 gaggctgcgt atgcgcccct cctgctactg actggacttc gccccctttg cttcccttgc
    21301 taaacaggtt tgatttcagg tcgacacgac cgcaaacctt attacgcagg ggaggcagca
    21361 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    21421 aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac gccgatgacc
    21481 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    21541 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    21601 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaatcc gcacgtcgga
    21661 tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat caacgttttg
    21721 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtggta
    21781 accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt cgctaacttc
    21841 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtggtt
    21901 cagggactgc ataaaggaga aggcattggc accgtctggt cacttcagcc gtttatcagc
    21961 gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt ctcgttcctc
    22021 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    22081 aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc gtcgttcttt
    22141 atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc actgcctgaa
    22201 attgcgctgt acgtcggcgg caagctcttt cagtcgattt tcctctgcac cacgtttgtg
    22261 aacacattag cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    22321 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    22381 ccggcactca acgtcattat ggtcgggatt gtcgcgctgt cggcgctgtt ctttgattta
    22441 gtcaccgcga cagcactgat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    22501 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    22561 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaaccta
    22621 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttgtgg
    22681 tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac gccggtgagc
    22741 gaaacgtaat agccggatgc gcggcgcatt atcctgcctg agggtaacgc gccgcgccat
    22801 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    22861 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    22921 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaacgca caccgctggc
    22981 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    23041 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    23101 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    23161 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    23221 taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag
    23281 ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    23341 aaccacacgg ccctcattac gaccctgaac acaaacggta atggcaaatg tcattaaata
    23401 ttagtaagat agcctcttta cataaaatcc cttaatatta tgcgccaggt attatcgtct
    23461 cttttggtga ttgctggact tgtgagtggt caggcaatcg ccgcgcctga atcccccaca
    23521 catgctgata tccgcgacag cggttttgtc tattgcgtca gcgggcaagt caacaccttt
    23581 aacccatcca aagcgagcag tgggttaatt gtcgataccc ttgccgccca gttttatgat
    23641 cgactgctgg atgtcgatcc ctatacctat cgcctgatgc cggaacttgc cgaaagctgg
    23701 gaagtactcg acaacggcgc gacctatcgc ttccacctgc gtcgcgatgt tccgtttcaa
    23761 aaaaccgact ggtttactcc cactcgtaaa atgaatgccg acgatgtggt gtttaccttc
    23821 cagcgaattt ttgaccgcaa caacccgtgg cataacgtca acggcagcaa cttcccctac
    23881 ttcgacagcc tgcaatttgc cgataacgtg aaaagcgtcc gcaaactgga taatcatacc
    23941 gttgagttcc gactcgccca gccggatgct tcttttttgt ggcacctcgc aacccattat
    24001 gcttcggtca tgtcggcaga atatgcccgg aagttagaga aagaagatcg ccaggagcaa
    24061 ctcgaccgtc aaccggtcgg caccggtccg tatcagttgt cggaataccg cgccgggcaa
    24121 tttattcgcc tacaacgtca tgatgacttc tggcgcggta aaccgttaat gccgcaggta
    24181 gtggtggatt taggctccgg cggcaccgga cgtctgtcga aactcctgac cggggaatgc
    24241 gacgttctgg cctggcctgc tgccagccag ctatccattt tgcgtgacga cccgcgcttg
    24301 cgtttaacgc tgcgtcctgg gatgaacgtc gcctatctgg catttaacac cgccaaaccg
    24361 ccgctaaata atcccgctgt ccgccatgca ctggcactgg cgattaataa ccagcgcctg
    24421 atgcaatcca tctattatgg tacggctgaa acggcggcct ctattttacc gcgcgcctcg
    24481 tgggcctatg acaacgaggc taaaattact gaatacaatc cggcgaaatc gcgcgaacag
    24541 ttgaaggcgt tggggctgga aaacttaacg ctgaaactgt gggtgcccac acgttcgcag
    24601 gcgtggaacc ccagtccact gaaaactgcc gaactgattc aggcggatat ggcgcaggtt
    24661 ggcgtaaaag tggtgattgt gccggtagaa ggtcgctttc aggaggcgcg gttgatggat
    24721 atgagccatg atctgacgtt atccggttgg gcgacggaca gtaacgaccc ggacagtttc
    24781 ttccgtcctt tactgagctg cgcggcaatt cattcacaga ccaacctcgc ccactggtgc
    24841 gatccgaaat tcgacagcgt gttgcgtaag gcgctctcct cgcagcagct ggcggcgcgt
    24901 attgaagcct atgacgaagc gcagagtatt ctggcgcagg aattgcccat tttgccgctg
    24961 gcgtcgtcat tgcgtttgca ggcctatcgg tacgatatca aaggtctggt acttagcccg
    25021 tttggtaacg cctcctttgc tggggtgtat cgcgagaaac aggatgaggt gaaaaaacca
    25081 tgattatctt caccttacgc cgcattttgt tattgattgt caccttgttc ctgctgacat
    25141 tcgttggctt cagcttgagc tatttcaccc ctcatgcgcc gttacaaggc gcgtcactgt
    25201 ggaatgcctg ggtgttctgg tttaacggcc tgatccactg ggattttggt gtttccagca
    25261 ttaacggtca accgattgcc gaacagttaa aagaggtctt cccggcgacg atggaactgt
    25321 gcatcctcgc cttcggtttt gcgctgattg ttggcatccc cgtggggatg attgccggga
    25381 ttacgcgcca taagtggcag gataacttga tcaacgccat cgccttactg ggcttttcaa
    25441 tcccggtgtt ctggctggcg cttctgttga cgctgttttg ttcactcacg ctaggttggt
    25501 tgcccgtttc agggcgtttc gatctgctct acgaagtgaa accgattacc ggttttgcgt
    25561 tgattgatgc ctggctttcg gactcaccat ggcgggatga aatgatcatg agcgcaatcc
    25621 gccatatgat attgcccgtg atcaccctgt cggttgcgcc aacaactgaa gtgatccgcc
    25681 tgatgcgtat cagcactatc gaagtgtatg accagaacta tgtaaaagcg gcggcgaccc
    25741 gcggtttgtc gcgctttact attttgcgtc gccacgttct gcataacgcg ttaccgccgg
    25801 ttattcctcg cctgggctta cagttttcca ccatgctgac gctggcgatg atcaccgaaa
    25861 tggtttttag ctggccgggt ttagggcgct ggttaattaa cgccatccgc cagcaggatt
    25921 acgcagccat ttccgccgga gtgatggtgt gtggctcact ggtcattatt gttaacgtga
    25981 tttctgatat tttgggtgcc atggctaacc ctctgaaaca taaggaatgg tatgccttac
    26041 gatagcgtat acagcgaaaa gcgcccgccg ggcacgctgc gtaccgcctg gcgcaaattt
    26101 tatagtgacg cctctgcgat ggtcggcctg tacggctgcg cggggctggc tgtactgtgt
    26161 atttttggcg gctggtttgc gccttacggc atcgaccagc aatttctcgg ttatcaatta
    26221 ttgccgccgt catggtcgcg ctatggcgaa gtttctttct tcctggggac tgacgacctc
    26281 ggacgcgatg ttttaagccg tttactgagc ggagctgcgc caaccgtggg tggcgcattt
    26341 gtggtgacgc ttgccgcgac gatctgtggc ctggtactgg gcaccttcgc cggggcgaca
    26401 cacggcctcc gctcagcggt gcttaaccat attctggata ccttactggc gatcccttcg
    26461 ctgctgttgg caattatcgt tgtagcgttt gccggaccga gtttgtccca cgctatgttt
    26521 gctgtctggc tggcattgct gccgcgtatg gtacgttcga tttacagcat ggtgcatgac
    26581 gaactggaaa aagagtacgt tatcgccgcc cgtctggatg gcgcatcaac gctgaatatt
    26641 ctctggtttg ctgtgatgcc aaacatcacc gccgggctgg tcaccgagat cacccgcgca
    26701 ctgtcgatgg caattctcga tatcgccgcg ctgggctttc tcgatctcgg cgcacaactc
    26761 ccctcgcctg aatggggagc gatgctcggt gatgcgctgg aactgattta tgtcgccccg
    26821 tggactgtca tgctgccagg tgcggcaatt atgattagcg tgttgttggt taacctcctt
    26881 ggcgatggcg ttcgtcgtgc gattattgcg ggggtggaat aatgccatta ctcgatattc
    26941 gtaacctgac cattgaattt aaaaccggtg atgagtgggt taaagccgtc gaccgggtaa
    27001 gtatgacctt aaccgaaggt gaaatccgcg gtcttgttgg tgaatccggt tccggcaaga
    27061 gtttgattgc gaaagcgatt tgtggggtga ataaagataa ctggcgtgtt actgctgacc
    27121 gtatgcgttt tgatgatatc gatttgctgc gtctctccgc acgcgaacgg cgcaaactgg
    27181 ttggccataa cgtgtcgatg attttccagg aaccgcagtc gtgtcttgac ccttcagaac
    27241 gtgtgggtcg ccagttgatg caaaacatcc cagcctggac ctataaaggc cgttggtggc
    27301 agcgttttgg ctggcgcaaa cgccgtgcca ttgaactgct gcaccgcgtg gggattaaag
    27361 atcacaaaga tgcgatgcgc agttttccct atgagttgac cgaaggtgaa tgtcagaaag
    27421 tgatgatagc cattgcactg gcgaatcaac cgcgtctgct gattgctgac gaaccgacca
    27481 actcaatgga gccaacaacc caggcgcaaa tctttcgcct gctgacgcgt ctcaaccaaa
    27541 acagcaatac cactattttg cttatcagcc atgacttaca aatgcttagc caatgggcgg
    27601 ataaaattaa cgtgctttac tgcggtcaaa cagtggaaac cgcgccgagt aaggagttgg
    27661 tgacgatgcc acatcatcct tatacccagg cgctgatccg cgcgatacca gacttcggca
    27721 gcgcgatgcc gcataaaagt cgcctcaata cgctgcccgg cgctatccca ctgctggaac
    27781 agttaccgat tggttgtcgt ctggggccac gttgcccgta tgcacaacga gaatgcattg
    27841 tgacgccacg tttgacgggg gcgaaaaatc atctctatgc ctgtcatttc ccgctgaaca
    27901 tggagaaaga gtgagatgat cgaaacgctg cttgaagtgc gtaatttaag taaaacgttc
    27961 cgctaccgga ccggctggtt tcgtcgtcag accgtagaag cggtaaaacc cttgagcttt
    28021 acgctacgtg aaggccagac actggcgatt attggcgaga atggttcggg taaatccacg
    28081 ctggcaaaaa tgctggcggg aatgatagag cctaccagcg gcgagttatt gattgacgat
    28141 catccactgc attttggcga ttattccttc cgcagtcagc gtattcgcat gatttttcag
    28201 gatccttcga cctcgttgaa tccccgtcaa cgtatctcgc aaattctgga ttttccactg
    28261 cgcctgaaca ccgacctgga accggaacag cgtcgcaaac agattattga aacgatgcgt
    28321 atggttgggc tgctaccgga tcacgtcagt tactatccgc atatgttggc acccggacaa
    28381 aaacagcgtc tgggtctggc gcgcgcgctg atattgcgcc caaaagtcat tattgccgat
    28441 gaagccctcg cttcactgga tatgtcgatg cgttcgcagt tgattaatct gatgctggag
    28501 ttacaggaaa aacagggcat ttcgtatatt tatgttaccc agcatatcgg aatgatgaag
    28561 cacattagcg accaggtgct ggtgatgcat cagggcgagg ttgtcgagcg aggcagcacc
    28621 gcggatgtgc tggcatcgcc gctgcatgga ctcaccaaac ggctgattgc cgggcacttt
    28681 ggcgaagcat tgacagcgga tgcgtggcgt aaagatcgct aattttctga atcctccttc
    28741 cagattatcc acctcattct gacaagagat taaatcttgt cagaatgagg tgatggataa
    28801 aataaaatca aatgcgcgtg atttaagact caatctcact ttgcaggaac gaaagctctg
    28861 gcgatatctt cgcagccgac gttttggtga tttcaaattt cgccgtcaac atccgatggg
    28921 gagctacatt ctcgattttg cttgctgctc ggcgcgcgta gtcgttgagc tggatggtgg
    28981 gcagcatgat ttggcagttg cctatgatac caggcgcact agctggcttg agtcgcaggg
    29041 ctggaccgtg ctgcgtttct ggaataacga gattgattgt aatgaggagg cggtgctgga
    29101 gattattctg caggaactga accgccgatc accctcaccc tgaaagggcg agggggccga
    29161 ccgagccgaa tagctgttgt ggtgaaaaca tggagacagt gctggagaat attcggcaag
    29221 gtctgaacca tcccagccat cgccataaaa gggttagggg ctgtatgagc ctgtttgttg
    29281 ctggggtaac aatatttgca caatatggtc ccctcgcccc tctggggaga gggttagggt
    29341 aaggggaaaa gcgcccaccc tgccgcagcc tgctccggtc ggacctggca actatagcta
    29401 ctcacagcca ggttgattat aataaccgtt tatctgttcg tactgtttac taaaacgacg
    29461 aatcgcctga ttttcaggca caacaagcat caacaataag gattaaagct atgggttttc
    29521 tttccggtaa gcgcattctg gtaaccggtg ttgccagcaa actatccatc gcctacggta
    29581 tcgctcaggc gatgcaccgc gaaggagctg aactggcatt cacctaccag aacgacaaac
    29641 tgaaaggccg cgtagaagaa tttgccgctc aattgggttc tgacatcgtt ctgcagtgcg
    29701 atgttgcaga agatgcctgc atcgacacca tgttcgctga actggggaaa gtttggccga
    29761 aatttgacgg tttcgtacac tctattggtt ttgcacctgg cgatcagctg gatggtgact
    29821 atgttaacgc cgttacccgt gaaggcttca aaattgccca cgacatcagc tcctacagct
    29881 tcgttgcaat ggcaaaagct tgccgctcca tgctgaatcc gggttctgcc ctgctgaccc
    29941 tttcctacct tggcgctgag cgcgctatcc cgaactacaa cgttatgggt ctggcaaaag
    30001 cgtctctgga agcgaacgtg cgctatatgg cgaacgcgat gggtccggaa ggtgtgcgtg
    30061 ttaacgccat ctctgctggt ccgatccgta ctctggcggc ttccggtatc aaagacttcc
    30121 gcaaaatgct ggctcattgc gaagccgtta ccccgattcg ccgtaccgtt actattgaag
    30181 atgtgggtaa ctccgcggca ttcctgtgct ccgatctctc tgccggtatc tccggtgaag
    30241 tggtccacgt tgacggcggt ttcagcatcg ctgcaatgaa cgaactcgaa ttgaaataat
    30301 cattctgttg gtaaatattg ggcggcgttc tgccgcccgt tatctctgtt atacctttct
    30361 gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt
    30421 tcctttacga ccaaggagcg cccatggaac aacgccacat caccgtcaaa agccactggt
    30481 atcatgaaac gcaatccagt actgcggagt atgacgttct gcctctggtc ccggaagccg
    30541 caaaggtcag cgatcccttt ctgctcgacg tgatccttga tgaagaaacg ctggctccct
    30601 tcctttcatg gctggtccct gcgcgcgttc ttgcagtgga attgttccct gaccagctta
    30661 ccgtgacccg ttcacagacc ttcaccgctt atgaacgctt gtctacggcc ctgacggttg
    30721 ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt acgccgctcc
    30781 ccgggcctga ttcctccagg gaaagcaatc ataggttggc acaaatcacg caatatgccc
    30841 gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg
    30901 tcggtcttac tgcctgggac tgtgtgatca ttaaccaaat cattggtttt attggctttc
    30961 aggcgcggac cattgcgaca tttcaggctt atctcgggca cccggtacgc tggttacccg
    31021 gtctggaaat acaaaactac gccgacgcgt cactgtttgc tgatgaatca atacgctggc
    31081 gaagcagcta tgaggtggaa aaactacctg aagagtacac aaaaagttca acagcagaac
    31141 tttgccaact ggccgaaaca ctctctctcc accctatttc actttccctt ctcgaaaggt
    31201 tgttaaacag tacacgggtt aatacacagc cggataataa gcttgcggcg ttgttatgcg
    31261 cgcggataaa tggcagtcct gcttgttttg ccgcctgtat ggattcatca aatgaatata
    31321 aaaaaatcag cccccttctg cgcaagggcg aaaatgaaat taaccaatgg gctgaccgtc
    31381 attctgttga gcacgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct
    31441 ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg cagattatta
    31501 atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg
    31561 agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg
    31621 ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa
    31681 ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc
    31741 ttcctggaag tcgacgcgca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc
    31801 atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag
    31861 ccagaagaac tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat
    31921 gaccgtctgg ccatcgttcc tgatcatcca ctcttaaaag acgccattcc ttgccgcgca
    31981 gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga aatgcgccgt
    32041 catccgctga aaggcgatcg ttctttctat gctgaattga cacaatacat cacttttggt
    32101 gacgaccact ttgtaccgtg gtgggttaca cttgcgcgcc ataatctgga aaaagaagca
    32161 ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgaccgcg
    32221 ctggattttg tcaccatcga cagtgccagc acagaagata tggatgacgc ccttttcgct
    32281 aaggcgttgc cggatgacaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg
    32341 attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg
    32401 cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc
    32461 gccaatgaaa tccgcccggt actggcatgc cgcatgacgc tctccgctga tggcaccatt
    32521 gaagataata tcgaattctt tgccgccacc atcgaatcca aagcaaagct ggtgtatgac
    32581 caggtttctg actggctgga aaataccggt gactggcagc ctgaaagtga agcaattgcc
    32641 gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg gcgagtggcg tcataaccac
    32701 gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg
    32761 ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt
    32821 gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac
    32881 gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacgcac
    32941 ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt
    33001 gaactggatg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt
    33061 gctgaaatta gcactgaacc cggtcctcac tttggcctcg gtctggaagc atacgccacc
    33121 tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt
    33181 atcaaaggcg aaactgcgac gcgcccacag gatgaaatca ctgtccaaat ggccgagcgt
    33241 cgccgtctca accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg
    33301 aaagacaaag ccgggaccga cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc
    33361 ggcatgcgtg tgcgtttggt tgataacggc gctatcgcct ttattccggc acctttctta
    33421 cacgctatgc gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt
    33481 gaaacggttt acaaagtaac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa
    33541 acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca ttcctcatgg
    33601 atgggccgtt tatttccccg ctctaccttc gttatatcca ggcagatttt ttaactgact
    33661 ttcgtttgaa aactggcgtt tttccaaaac tggattacaa ataaatatat aaaaaaaaca
    33721 atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga
    33781 ctccgctgtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta
    33841 caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgttttgcg
    33901 cttttctacc accgaaacca cagaacctga tcgtacattg cagttatctg ccgaacaggc
    33961 tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga
    34021 tgaaagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg
    34081 caacgcgtct gcatggcatg acacaccggc ggttgctcgt gatttatcac acgggctttc
    34141 ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg
    34201 gaatatccaa cgcttcaatc ggttatgtga agattacaca gggttgaaag aacacgacgt
    34261 cattgggcaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg
    34321 caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac
    34381 acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa
    34441 aaacgagatt tttttaatct gttccggtac cgacattacc gaaggcgccg cgctcaggag
    34501 cgactgcgta ttctggcaaa taccgacagt atcaccggac tgccgaatcg taacgcaatg
    34561 caggagttaa tcgatcacgc tattaatcag gcagataaca ataaagttgg ggttgtgtat
    34621 cttgatttgg ataattttaa aaaggtcaac gacgcctatg ggcatttgtt tggtgaccag
    34681 ttattacgcg acgtgtcatt ggctatttta agctgtctcg aacatgacca ggtgttggcg
    34741 cgtccaggtg gcgatgagtt tctggtactg gcatccaata cctcacaaag cgcgctggaa
    34801 gcaatggcat cacgaatttt gacccgctta cgcctcccct ttcgtattgg tttaattgaa
    34861 gtttatacca gctgttcagt aggtatttcg ctctctcccg aacatggttc ggacagcgcg
    34921 actattattc gtcacgccga cacagcaatg tacacagcga aggaaggcgg acgaggacaa
    34981 ttttgcgttt ttactccaga aatgaatcag cgggtatttg aatatctctg gctggatacc
    35041 aacttgcgta aagcactgga aaacgatcag ttggttattc actatcaacc gaaaatcacc
    35101 tggcgtggcg aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg
    35161 ttgattccac cgttggactt catttcctac gccgaagagt cagggctaat tgtgccttta
    35221 ggccgttggg tgattctcga tgtcgtacgc caggtggcga agtggcggga taaaggcatt
    35281 aacctgcgag tggcggtaaa catttctgca cgtcagctcg ccgatcaaac cattttcacc
    35341 gccctgaaac aggttctcca ggaactgaat tttgaatact gcccaataga tgttgaactg
    35401 acagagagtt gtctgattga gaataatgaa ctggcactgg ctgttattca acaatttagc
    35461 caactaggtg cgcaagtgca tctggacgat tttggtaccg gctactcttc tctttcgcaa
    35521 ctggcgcgct ttccgatcga tgccatcaaa cttgaccagg gttttgttcg agatattcac
    35581 aaacagcctg tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat
    35641 cttcaggtga tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac
    35701 gggatcaatg agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa
    35761 cgttggtata aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgtta
    35821 tattccgtca ttatatatcg tattccagga tgataattag ccgttgctgt taaacacttc
    35881 tcttttagcg gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg
    35941 atgtctgaat ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt
    36001 gtggcaggcg attaccactc tgcgatccat aatctcgaga tacttaaagc agaactcttg
    36061 cgccaggttg ctgaatctac gccagacatc ccgaaggcac cgtgggagat ttaacaatcg
    36121 tgccttcagg ttttcctatt tgaggatgcc cggcgcgtca gtattttttt atttagtatt
    36181 ataacgttat aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca
    36241 ttgaccaggg tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta
    36301 ccattcgcca ggatctcaac accctcgaaa aactgagtta cctccgccgt gcacatggct
    36361 ttgcagtttc gcttgatagt gatgacgtcg aaacccgtat gatgagcaac tatacgctga
    36421 agcgtgaact cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc atctttatcg
    36481 aaaatggtag cagcaatgcc ctgctcgccc ggactctggg cgaacagaag aaaaatgtca
    36541 ctatcatcac agtcagcagc tacattgcgc atttgctgaa agacgcgcct tgtgaagtca
    36601 ttttgctcgg tggcgtgtac cagaaaaaaa gcgaaagtat ggttggccct ttgacacgcc
    36661 agtgcatcca gcaggtacat ttcagcaaag catttattgg tattgatggc tggcaacctg
    36721 aaactggatt caccggtcgc gatatgatgc gtaccgatgt ggtcaatgcc gtgctggaaa
    36781 aagagtgcga agcgatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact
    36841 ccatcggtcc cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg
    36901 atctgatgca tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc
    36961 ccttctctac ggatgatttg cagtttggca aatcatccgc tctaagatga ttcctggttg
    37021 ataattaaga ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat
    37081 ataacaggaa gtcattatca cctgcgtgat ataaccctgc gcgcgagcag atttcacgga
    37141 ataatttcac caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc
    37201 ataaattcag gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg
    37261 gcaattactt tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac
    37321 accgcaatcg gcgcgggtgc aggggcatta ggcggtgcag tactgaccga tggcagtacg
    37381 ttgggtacat taggtggtgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa
    37441 tcgtaaataa atgtcgatta tcgactcgca attataatac gcttgattct gttcagtacg
    37501 aaataactga ataatacaca cagtaaaata ttcgaaagcc acggatatat ccgtggcttt
    37561 ttatgttaac cgcctgcgag ttttaccttc atccctttcg cttcaagtag tgattttaat
    37621 aaatcacgct tatcgccctg gatttcaata actccatctt taactgctcc gccgcagccg
    37681 cattttttct tcagttccgc tgcaagtttt gtcagttcgg catcatcgag atcgataccg
    37741 gtaatcaggc aaacgccctt acctttacgt ccactggtct gacgctgaat acgcaccaca
    37801 ccatcgcctt tagggcgtac tggggccgct tttggctcat caatacgtcc ggtttccgtt
    37861 gagtagacca gacggctgtt ggaatcactc atcatgcact ccgctgtaaa gaggagttga
    37921 tcgctttcag tgtttgcgct ggatctaccg attgcgttac cgggcgacca atcaccatat
    37981 aatcaacacc agccgccaac gcctgttctg gcgtcataat gcggcgctgg tcaccagcat
    38041 cactcccctg cggacgaatg cccggtgtaa ccagtttgaa ctcctgaccg aatacctgtt
    38101 taaagcgcac agcttcctga gcagaacaca ccacaccatc aaggccacat ttttgcgtca
    38161 gtgccgccag acgttctgca taatctgcag gtgacagtgt cacgccaaga tcggccaggt
    38221 cgctagcttc catgctggtc aacactgtta cagcaatcaa aagcggtgca tctttgccaa
    38281 acggaaccag tgcctcacgc gctgcggtca tcatacgcgc cccaccagag gcatgaacat
    38341 tcaccatcca cacgcctaag tcagctgcag cagcgacagc gtgcgctgca gtgttaggaa
    38401 tatcgtggaa tttcaggtca agaaagatat caaaaccacg ctgttgaagt tcgcgcacaa
    38461 actgtggccc aaacaatgta aacatctctt tgccgacctt cagacgacaa tcgcgtgggt
    38521 cgatcttgtc gacaaaggac agcgcgtcat cacgattatg ataatcaagg gcaacaacca
    38581 caggagaatt cgtaacagcg cgggaagaag atgaagcagt taacgtcatg accagacctt
    38641 cttgatgatg ggcaccggaa gtggcgcgaa acaggtaaac ggcgagcatt ctacccgtgg
    38701 aaataaaaaa ttaacagttg cgatttttcc cctggcaggc tttttctgct ccgtcgttgc
    38761 cggagcacaa tggaacatca ataactagta tgttgtaact aaagtcggat ttttttaaaa
    38821 attacaggcc atcaagaccg cgaatcggtt taatggttga ccaggcccga caagacggac
    38881 aatgccagta gagggtgtat gcggtaaaac cacatttctg gcagcgataa cgaggcttac
    38941 tacgtacctt ttcgccaacc atgtcacgca gcaccatcag gctctctttg gcacgccctt
    39001 cttccgcttc atttaagtgg taatccatta acttatggaa cacacgcatg gtcggatgac
    39061 gctgaagctg gcgcgtaata tagacctgtg cggcctcact accgtcgcgc gcttcgatga
    39121 tatcagcaag catcaattca gcatcggcac cggtgttctc ttccaccgcg cgttgcagga
    39181 attctgccca ttcggcagtt ttacccaact gctgatagca ggtttgcaac atttccagcg
    39241 tttcgctgac cagttctctg tcctgggata tcacgcgttg caaactttcg acggctttgg
    39301 cgtattctcc tttcgccata aacacgcgtc ccatcatgat ggatacgcgg gcgctgtttt
    39361 tatctgccgc cgcccctttt tttagcaagg tcatggcacg atcgagatcg tcgctggcca
    39421 tatgctgcag ggctaactca cagtagaaat gggcaatttc gacgcgctgt ttatctttac
    39481 ccagcttcac caggcgttcg gcaacatcaa ttgctttctg ccactcactg gtagcctggt
    39541 agatttgtag caactgttgc agcgcgccaa tgcggaagtc agtttcatcg gtcagctgat
    39601 tgaacatgtc ttccgcgcgg tcgtataacc cggcggccat gtaatcacgc cctagttgtt
    39661 gaatcgccaa cagacgctgt tcataggtca gcgaggcgct ttccattagg gtctgatgga
    39721 tgcgaatagc gcgatcaact tcgccacgcg aacggaacag gtttccgagc gtaaggtggg
    39781 cttcaacggt gcctgtatcc tctttaagca tatcgagaaa caggtctacc gctttatcct
    39841 gttgattact aagcaggaag ttaacccccg ctacgtaatc acgcgacaag cggttggctt
    39901 catcttgctt gttttgttgc gcacttctgc ggcccatata ccagccatag gcagcggcta
    39961 caggcaaaag cagaaacaac aactccagca tagaaagtta ttccttcgcc gctgacgagt
    40021 gcggcagtac agccacgtca gtcgcgggtg aaagctggtt ttccagtcgc tttattttac
    40081 gttcagcgcg cgccagggaa acacgaactc gcagccagaa caggccacaa atcaaccaac
    40141 cgatagcaaa ccccgcagca aacaataccg ccagcaatgt ggaaatacgg tactcccctt
    40201 gcgctaacag ataattaaac gtcacctgtt gatcgttctg cgcacccaac gtgaccgaaa
    40261 tcacgaagat cgctaacacc agtaagaaaa tgagtaaata tttcacatta cttcccgtta
    40321 tgtggttaga gcgaataaat tatggtcaac ttaccgcaat agccctttaa agtaccattt
    40381 tcgcgccgtc tgcgaaatgg catccacgcg cattgttgat gatttaaggg ctaaattcgg
    40441 aaaatcaacc agcgttaact ttcttgttct cgttgcgcta tttcgcgatt ttcttccgca
    40501 ggtggtgtta atggcccaca aattcgttgc gcaagccacg ttgccaccgc caccagcgcc
    40561 cacgaaatca acgtagctac taccagatcg cgtggccaat gcatcccgag cagcaggcgg
    40621 cttcccatga ctcccgttgc ccagaccagc aagatagcaa tggttaacgt tcgccgacgc
    40681 ggccacagca aaccaacggc cagcagtgcc caactggcag caaacatcgt gtgaccggaa
    40741 ggaaaggcaa accccgtctc tttctgccag tgtgaacgca aatattgtgg gatatttttc
    40801 tcttcagcca actgttcttt cactagattt ccgcgttctg ctcgctttaa agtgtagaac
    40861 tcatcaaccg gaatatgatg tgttttttcc agccagataa caaaaggtcg tggttcctgg
    40921 actttgtctt tgatccagga tttaacgcct tgtcccacaa ggattgcggc cgccagaatg
    40981 gcaaataata caaaggcagc cttaatgcga aaacgcagac accagagaaa ccagccgaat
    41041 aaaatcaaat gtgtaatgac gccccagggc tgggtgacag tttcagtaac ccaaaaagcc
    41101 gcttttagta gccaactttg ttctccaggt tgccaacgcc agccagaaat ccatacggct
    41161 actggcatga caagcaatag tgcagctccc actgcggtac gtctggcaat cgaacgcatg
    41221 gcctctcctt tttgataagt cccacaatca taactgaaaa cgccagttcc aggaaaaatt
    41281 gacagatttg tgccattccg tgaacgatcg acgcgtcgtg attaggtgaa ccccttctcg
    41341 ttatggcaaa ataagccaat acagaaccag cattatctgg agaatttcat gcagcttaaa
    41401 cgtgtggcag aagccaaact gccaacccca tggggcgatt tcctgatggt gggatttgaa
    41461 gaactggcaa ccggacacga tcatgtcgcg ctagtctatg gcgatatttc cgggcatacc
    41521 ccggtacttg cgcgcgtcca ttccgaatgt ctgaccggtg acgccctgtt cagcttgcgc
    41581 tgcgattgtg gcttccagct cgaagcggca ttgacgcaaa ttgccgagga aggccgtggt
    41641 attttgctgt atcaccgtca ggaaggtcgt aacattggtc tgctgaataa aatccgcgct
    41701 tacgcactgc aggatcaagg ttacgatacc gtagaggcta accaccagtt aggcttcgcc
    41761 gctgatgagc gcgacttcac tctttgcgct gatatgttca aactccttgg cgtcaatgaa
    41821 gtccgcttgt taaccaataa cccgaaaaaa gtcgaaattc tgaccgaagc agggattaat
    41881 attgttgaac gcgtaccatt gattgtaggt cgtaacccca ataacgaaca ttatctcgat
    41941 accaaagccg agaaaatggg ccatttgctg aacaaataac cctcttgcat tgtgtaatta
    42001 atttgcttgc cggaagcaaa ataaccggca agcaaatact tgttacttca acatattacg
    42061 aatgacataa tgcaaaatgc cgtcgttctg gtagtaggtc aactccgtcg cggtgtcgat
    42121 acgacaacgg cagggtacga cctcctggct accatccgcg cgcgtaagcg tcaccggaac
    42181 cgtcgcaccg ggttgtagat tttgcagatc gccaatatca atcttctctt ccccggttag
    42241 tcctaacgtt ttacgcgtta cgccttgcgg aaattccagc ggcaggatgc ccatgccaat
    42301 taaattcgaa cggtgaattc gttcaaacga ttcggcaatc accacacgaa ctccaagcag
    42361 acgaggacct tttgccgccc agtcacggct tgagcctgat ccatactctt tcccggcaat
    42421 caccgccagc ggcgtttgtt cctgcttata gcgcatcgca gcatcataaa tagagactac
    42481 gtcgctgtca ggtaaatgcc gcgtcatccc cccttcaacg ccaggcacca tttcattacg
    42541 gatgcgaata ttggcgaagg tgccgcgcat catcacttca tggttaccac gccgcgaacc
    42601 gtaggagtta aagtcttttc gctcaacccc ccgaccttgt agatatcgac ccgctgggct
    42661 gtcgggctta atactgcccg ccggagagat atggtcagtg gtgactgaat cccccagcat
    42721 tgcgaggatc cgcgcaccgt gaatatcttc cactggtgct ggtgttgcct gcatttcatc
    42781 aaagaaaggc gataagcgaa tataggttga gtcctcctgc caaccgtagg tatcggatcg
    42841 tgtgacgtta atttccttcc actctgctgt gccttcaaaa acttctgcgt actctttgcg
    42901 gaacatttct gtggagactt gttctaccgc acgggcaatt tcttgtgccg atggccagat
    42961 atctttcaga tataccggat cgcctttacg atcatggccg ataggctcag aagccaggtt
    43021 gatattcata tttcccgcca gcgcataggc aaccaccagc ggcggcgagg ccagccagtt
    43081 ggttttaacc agcggatgga tacggccttc aaagttacgg ttgccggaca gcactgcacc
    43141 gactgttaaa tcgccttttt tgattgccgt ttcgatagga tcgggcagcg ccccagagtt
    43201 accaatacag gtggtacaac cgtatcccac aaggttaaac cccagttcgt cgagatacgg
    43261 tgtcaatttc gcttttgcca gataatcaga aacaactttc gagcctggtg ccagcgacgc
    43321 tttgacccac ggttgccgct tgaggccaag agttacggct tttttcgcca gcaagcctgc
    43381 agccatcagc acacttgggt tagaggtgtt ggtgcacgag gttatcgcag caatgaccac
    43441 agcgccatca ggtaactgat actgatgtcc gttcataaca taatcgaccg gctggcgatc
    43501 tttatgcgtg gcattcactt ccagttcgtt actggcggca aatgcttttg gtacatcggg
    43561 cagtgcaacg cgatcctgtg ggcgttttgg ccctgccagg ctcgcttcaa cgtcattcat
    43621 atccagttct aacatactgg taaaaatcgg ttcatcgccc gggttacgcc acatgccctg
    43681 cgctttggca tatttttcga ccaactcgac ctgatcttcg ctgcgcccgc ttaaacgcat
    43741 gtaatcgagg gttactgcat cgattgggaa gaagccacag gtggcaccat attctggcga
    43801 catattggca atggtggcgc gatccgccaa cggtagtgaa tccagaccat caccataaaa
    43861 ttcgacgaat ttccccacca cgccatgttt gcgcagcatt tgggtaacag tgagaaccag
    43921 gtctgtggcg gtaatacctt cacgtaattt accggtaagt ttaaagccca ctacgtccgg
    43981 gataagcatg gaaaccggct ggcctaacat tgcggcttct gcttcgatcc cacccacgcc
    44041 ccaccccagc acgccaaggc cgttgatcat agtggtgtgc gagtcagtac caacgagtgt
    44101 atccggataa gcaatccatt caccgtcctg caattcactc cacactgctt tgccgagata
    44161 ttcgaggtta acctgatggc aaatgcctgt gcctggcggc acgacgctaa accgactgaa
    44221 cgcttgcttt ccccatttca ggaacacata acgttcgtgg ttgcgctcca tttccaggcg
    44281 tacgttttct tcaaatgcct catcatcacc aaaacgatcg acggtcaccg agtggtcaat
    44341 gaccaggtcg accggtgaga gcgggttaac ctttgcagta tcgccgccga ggcgtttaac
    44401 cgcttcgcgc attgccgcca gatcaacaac ggcaggtacg ccggtaaagt cctgcatcag
    44461 cacccttgcc gggcggtagg caatttcacg gtcagcatgg gcatttttca gccatcctgc
    44521 cagcgcgtgg atatcctctt cggtaaccga gttaccatcc tgccagcgca gcaggttttc
    44581 gagcaaaact ttgagtgact tgggtagacg ggtgatatcg cccagtgatt tagcagcaag
    44641 cggcaggctg tagtagtggt aagttttatc tttggcctgc aacgtgtcct tactggcttc
    44701 tcgtagggtt gacgacatag ctcctcctta atgacagggt tgcgaagccc tgattctctt
    44761 cagggtgaat attaaagata acacaaacat cgcgtaacga tttgataaca acccaaattg
    44821 ataaaagagg agaaaccttg tggcaaagca aatgacaacc ccgccgcagc ggggtcaagg
    44881 atcagtgagt aatgatccag aggagttcgg cccagaagag catcgacaga gaaaacgcgc
    44941 ccatccagga ccaatatttc atatttgtgt tgatcatagg ctacaccctt aacggttatt
    45001 attttgtgtt gagataactc aagctgttat tgctgcggtg tattaaaagc cctttaattc
    45061 attaaaaatg ggtaataaga atgatgataa ttattgtttc ggctgatcat cttcagcaaa
    45121 cagttctata aaggcttgct gctgcgatgt tagttgccag tcgtctggaa taacgacgga
    45181 acattcccgt ttcgtttctg tttcattgtc actatgctgg cataatggtg acgactccag
    45241 ttgctcctga ccgaccatag ttatccccac actttccaaa tcagcagtgc gacaaccacc
    45301 cagaaaagtg cagaaccaag aaaaaccgcc agccaggctt tacgttttaa ttcaggatcg
    45361 cggcggggtt cctgatttcc agaaggcatt gctaacctca tataatcgcc atcacttatc
    45421 agcaagacgt tgaacgattg gtgtaattaa tcatacgatg agacaaatcc taaagaaact
    45481 ttagtcaaaa atccagcgaa tttacgaatc gtctccagtg aataattcaa tcttttgacc
    45541 tgaaatagat atttgaaaac tggagtttgc gaggcgggta attagacact actttacagg
    45601 taatttaccc tataaatatg agggataaaa atattgtgca cggtatagaa ataagtaaca
    45661 aaagagaata tattccggca ccttcgctac ataaaaggtg ccgaaaataa cgcaagaaat
    45721 tatttttccg gcagttttat atctttaaac atgacctcaa tttcttcatt agagcgcaat
    45781 gcgacagccg catcaacgac atcacgcgtt aaatgcggtg caaaacgctg aatgaaatca
    45841 tacatataac tgcgcaagaa agtactacgg cgaaaaccaa ttttggttgt actgtggctg
    45901 aagatatcgt gagcatcaac acgcacaagg tcgggatcgg cgaccggatc caccgccatg
    45961 ctggcaatga cccctacccc cagccctaac cggacgtaag ttttaatgac gtcagcatcc
    46021 gttgccgtga agacgatacg cggcgttaac cctgcgcggt taaaggcagt atccagttct
    46081 gaacgtccgg taaagccgaa ggtatatgtc accaacggat attgcgccag ttcttcaatg
    46141 gtaatggctt ttttgcctgc cagcgggtga tccggagtga ctacaatagc ccgattccag
    46201 tggtagcacg gtaacatcac taaatcttca tacagatgca gcgcttctgt ggcgatagcg
    46261 aaatcagcat tgcctttaga gacggcatca gcaatttgtg tcggcgagcc ctggtgcata
    46321 tgcaaagaaa cgcggggata acgctcaata aagcctttga taacgtttgg taacgcgtag
    46381 cgtgcctggg tatgcgtggt ggcgatatac agcgagcctt tatccggcca ggtgtgctca
    46441 ccggcaaccg attttatggc atcgactttc gacaggactt cgcgagcgat acgaattatt
    46501 tcttgtcctg ctggcgttac ctgcgtcagg tgcttgccgc ttcgggaaaa aatttgaatg
    46561 cctagctcgt cttccagcat tctgacttgt ttactgatcc cgggttgtga tgtgtaaagt
    46621 ccttccgctg ttgatgagac attcaggtta tgattgacca cctcaacaat atagcgaagt
    46681 tgttgtaatt tcatgttaaa ccatccactt agacgccatc agaacatcgt tttttcgtca
    46741 cacctggcgg gtgactaata cgatttaata ataaaaaagg tgctaactat aaccactata
    46801 tcatttatag ccttagtgta taggtgtgaa gaaaaaataa tatgcaataa aaaagggccg
    46861 ctttcgcgac cctttgttta taaaaacctg acagaattaa aggttatttt tttccttcaa
    46921 cccatttgcc atcaacataa aatgctgacc agccagtcgc ctttccgtct ttttccgaag
    46981 agacatattg ctgtttggtt ttacggctaa agcgaaccat ggtcttatta ccttccggat
    47041 cctgctgtgg cgcatcggcc agataacgca gtttttccgg cagacggtcg cggaagcgat
    47101 aaagctcttc caccagtggc gcacgcgttt cacgcgattt cgggaaagtg ttggcagcca
    47161 ggaacacacc ggcagcaccg tcacgcagca cgaaataagc atctgatttt tcgcacggca
    47221 gctcaggtaa tggcaccgga tcttctttcg gtggtgccac ttcgccgtta cgtaaaatct
    47281 tacgtgtgtt tttacactct tcgttggtgc aggccatgta tttaccgaaa cgccccattt
    47341 tcaggtgcat ttcagagcca catttttcac actcaacgat cgggccgtca taacctttaa
    47401 tgcggaattc gccctcttcg atctcgtaac cgtcgcaggt tgggttatta ccacagacat
    47461 gcaacttacg tttcggatcg atgagatagc tgtccatcgc cgtgccgcat ttcgggcaac
    47521 gacgttttgc gcgcagcgcg ttggtttcag catcttcgcc ttccagcacg ttcagcactt
    47581 cgttttccgg caccaggtta atggtggttt tgcaacgctc tttcggcggc agcgcatagc
    47641 cagaacagcc aaggaatacc ccggtgctcg ctgtgcgaat ccccattttg cgaccacaag
    47701 tcgggcagtc aatgctggtc agaaccatct ggttcgggcg catacccccc tcttccggat
    47761 ctttttcagc tttatctaac tgctgggtga aatccgagaa gaagtgatcc agtacagctt
    47821 tccactctgc ttcgtgattt gccacctggt cgaggctgtt ttccatctgc gcggtaaagt
    47881 cgtagttcat taactcgcgg aaattttctt caaggcgatc ggtgacgatt tcgcccattt
    47941 tttccgcata gaaacgacga ttttctactc gcacgtagcc acgatcctga atggtcgaaa
    48001 tgatcgacgc ataggtagac ggacgaccga taccacgttt ttccagctct ttaaccagcg
    48061 atgcttcact gaaacgggct ggcggcttgg taaagtgctg ggctggtgta agttcaacga
    48121 gcgtcagagc atcgccttta ttaactgctg gtaagatgcg atcttcatcg cctttacgca
    48181 acgcaggcat cacttttgtc cagccatcaa aacgcaaaat acgaccgcgt gctttcaggc
    48241 ggaaatcgcc cgcaccaacg gtcagcgtcg tggagtcata tttcgctggg gtcatctggc
    48301 aggctacgaa ctgacgccag attaactggt acagtttctg cgcatctgct tccatatcct
    48361 tcagcgattc cgccatcaca ttgacgtcag aaggacgaat cgcttcgtgc gcttcctgtg
    48421 agttttcttt gctggcgtac tgattcggac tttccggcag atatttctta ccaaaattat
    48481 cgctgatata accgcgaacc atatttaccg cgtcctgact caggttagtg gagtcggtac
    48541 gcatgtaagt gatatagcct gcttcataca aacgctgcgc catcatcatg gtttttttca
    48601 cgccaaatcc aagacgggtg ctggcagctt gttgcagcgt agaggtaata aaaggagcgc
    48661 caggtttact ggttgtcggt ttgtcttcac gttccagcac gctgtagcgc gctttttcca
    48721 gcagacttac cgcagcctga gtttgttctt tgttgaccgg acggaacggt ttgtcgttct
    48781 gatgagtcac ctgtaacgcc aacgcttcac cagatggcgt ggtcgtgctg gcatcgactt
    48841 cccagaactc ttccggcacg aacgctttaa tttcacgctc acgctcgacc accaggcgaa
    48901 ccgccaccga ctgcacacga ccggcagaca gaccacgagc gatctttttc catagcagcg
    48961 gcgaaaccat atacccaacc acgcggtcca taaagcgacg cgcctgctgg gcattaacac
    49021 gatcaatatt cagctcaccc ggtttgttaa atgcctggcg gatcgcgttt ttagtaattt
    49081 cgttaaacac cactcggcta tagcgcgcat catcaccacc aatcacttcc cgcaggtgcc
    49141 atgcaatggc ttccccttcg cggtcaaggt cggttgcgag atagatgtgg tcggcttttt
    49201 cagccagttg tttcagttca gagacgacct tctctttacc aggcaacact tcatagtgcg
    49261 cctcccaatt gtgccacggg tcaaccccca tacggttgac gagagcgcca cgttcatcct
    49321 ttttaggctt tttagccgtc ttggtggagg tagagtcggc actctttttg gcagctgagc
    49381 cactggttgg caaatcgcgg atgtgaccga cgctggattt caccacgtag tcactaccca
    49441 gatacttgtt gatcgttttg gcttttgccg gggactcaac gatgacaaga gctttaccca
    49501 tattcacctt acctaatttg attcttccag gaatgcgtcg cacgttgata aaccttccac
    49561 tggcaacgag ttaccgatat ggactcgttc tctgcggata tcaacccctt gtcgtgactc
    49621 tgttcacgaa aattcaggtg actgagttaa caggtcttgg cctacaggcg ctatagcacg
    49681 acggaatttt ggtcgaatgt caagcaaatc tattgccaga ttgacgaaag cgtcacactg
    49741 tacctgataa aatccgatac gcaactttat tagcatgcaa aatccaaagt taccagtaag
    49801 gcgctcatgc caccagcgtg gtatatttgt ctgcaagcca tgcctggcgt agactaatgt
    49861 ccttgtttaa ggagtaaata atgaacaaag agacacaacc catcgaccgc gaaacgctgt
    49921 tgaaagaggc caacaaaatc attcgtgagc atgaggatac gctcgcaggg attgaagcca
    49981 ccggcgtcac gcagcgtaat ggcgtgctgg tatttaccgg cgactacttt ttagatgaac
    50041 aaggattgcc aacagccaaa agcaccgcgg tgtttaatat gttcaaacac ctggcccatg
    50101 tgctttcaga aaaatatcat ctggttgatt aaagcaaaac gcgaggtctt agcctcgcgt
    50161 ttgtctttta catcaatggc ttttgacccc gctgccacca gcgtagcaac aatcgatcgg
    50221 cgctctctgc cgcgctgccg gtgaatcggt caatgagtcg tttacgctgc atatagcgta
    50281 cattaaccac ttcacggcct tccatcaggc taagaataac ttcatcacta gtgttgattt
    50341 catcaaccag gcctttctct accgcctgtt gtccgtacca gtgttcaccc gttgccacct
    50401 gttcaatatc cagagacgga cgcatacgct tcacaaaatc tttaaataac tgatgcgttt
    50461 cgttcagctc ttcgcggaat ttctcccgcc cttcttcggt gttttcaccc agcaaggtca
    50521 gcgtgcgctt atactgcccg gcggtgtgca gttcgatatc aatatctttg cttttcagga
    50581 agcggttaaa gttgggcatt tgcgccacca cgccaatgga acccacaata gcaaacggtg
    50641 cggaaacaat tttgtccgcc acacaggcca tcatgtaacc gccgctggca gcgactttgt
    50701 ctaccgtaac agttaaagga atgtttttat cacgcagacg ctgcagctgc gaagccgcca
    50761 acccgtaacc atgcaccatg ccaccagggc tttccagacg tagcacaacc tgatcctgcg
    50821 gtttgaatgc tgcgagtaca gccgttatct cttcacgtag cgagttcact tcatgggcgt
    50881 ccatgctgcc tttaaaatcc agcacccaga cgcgaggttt actgtcagtt gccacctcgc
    50941 ccagtttggc tttcgctttt gctgctttcg cttcttgctt gtgctttttc ttctgcgctt
    51001 tgtgccactg tttttgctga tgtgagtcca tcagcgccgc ggccagttct tctttcatct
    51061 ccttatactg ttcgctgaga ttgttgaccc gtaactcgcc acgctggcgt ttattacgtt
    51121 gagcaacatt gacaataatg gcggcaatcg ccgcaatcgc tagcacaacg gtaacgattt
    51181 tcgccaaaaa caaaccatat tcagacaaca attccacgag tccccacctt ggttaaacaa
    51241 cgctttggct cacagtttac aacaggcatg ttaagccgtc tcgcagagat gttattgcag
    51301 cgaaagtatg acaaagtatc cattttgcgg tgttatcgcc ccaggctgat tgaaatgccc
    51361 ttctgtttca ggcataaagc cccaaagtca taaagtacac tggcagcgcg acagacaacg
    51421 tcgttgagga gtagccatgc attaccagcc aaaacaagat ttactcaatg atcgcattat
    51481 cctggtgacg ggagccagcg atgggattgg tcgtgaagcc gcgatgacgt atgcacgcta
    51541 tggtgcgaca gtgattctgt tgggccgtaa tgaagaaaaa ttacgtcagg tagccagcca
    51601 cgtaaacgaa gaaactgggc gtcagccaca gtggtttatt ctcgatttgc tgacctgcac
    51661 gtccgaagat tgccaacaac tggcacagcg cattgccgtt aattatccgc gtctggatgg
    51721 tgttttgcat aatgccggat tgctcggcga tgtttgtcca atgagcgaac aaaatccaca
    51781 ggtctggcag gacgtcatac aggtcaacgt taatgccacc tttatgctca cccaggcact
    51841 gcttccttta ttactcaaat cggacgccgg ttcactggtc tttacttcat caagcgttgg
    51901 acgtcaggga cgagccaact ggggcgcata tgcagcgtcg aaatttgcca ccgaagggat
    51961 gatgcaggta ctggccgatg aatatcagca gcgcctgcgc gtcaactgca ttaacccagg
    52021 cggtacgcgc accgcaatgc gtgccagcgc cttcccgacc gaagatccac agaaacttaa
    52081 aacccccgct gatatcatgc cgctctacct ctggctgatg ggcgatgaca gccgccgtaa
    52141 aaccggcatg acctttgacg cccaaccggg ccgtaaacca ggaatttccc aatgagtgat
    52201 gaacgctacc aacagcgtca gcagcgagtg aaagaaaaag tagatgctcg tgtggcccag
    52261 gcccaggatg aacgcggtat tatcatcgtc tttaccggca atggaaaagg caaaaccacc
    52321 gcggcatttg gtacggcaac acgcgcagtt ggtcacggaa aaaaagtggg cgtcgtgcag
    52381 tttattaaag gcacctggcc taatggcgaa cgcaatctgc tggagccaca tggcgttgag
    52441 tttcaggtga tggcaacggg ctttacctgg gatacacaaa accgcgagtc tgataccgcc
    52501 gcctgccgcg aagtctggca acatgcaaag cggatgcttg ctgattcctc actggatatg
    52561 gttttgcttg atgaactgac gtatatggtg gcgtatgact atttgccact ggaagaagtg
    52621 gtgcaggcgt taaatgaacg tccacatcaa cagacggtga ttatcacggg tcgaggctgt
    52681 catcgggata ttcttgaact ggcggatacg gtaagtgagc tgcgccccat caaacatgcg
    52741 tttgatgccg gtgttaaggc gcaaataggg attgattatt aatctgtaaa aatgccggat
    52801 atgacgtccg gcatactttg attaaccgtt attacgcccg ccagaacgac gaccgccgct
    52861 cacctgactg tgacgtttca ccgcacgacg aatctgatta gccttcatgc gacgacggtc
    52921 tttttctaca gcgactttag agctggtttc tggcggaagc tccaccagtt cgcgcagata
    52981 gttagtctgg gcgaggtcca gctccgtcca gccaccgcgt ggcagacctt ttggcagtgg
    53041 gatatcaccg taacgaacac ggatcaggcg gctcacctgc acaccaaccg cttcccacag
    53101 acgacgaact tcacggttac ggccttcggt cagagtcacg ttgtaccact ggttgatccc
    53161 ttcgccgccg ctgaacttga tggttttaaa agctgccgga ccatcttcca gttgcacgcc
    53221 acgactcaaa tcacgcagtt tcgcgtcgtc aacctgacca aatacacgca cggcgtattc
    53281 acgttcaact tcacggcttg gatgcattaa acggtttgcc agttcaccat cggtggtgaa
    53341 cagcagcaga ccacaggtgt taacgtccag acgacctacg gcaatccagc gtgcgccacg
    53401 cagctttggc agacggtcaa acaccgtcgg acgcccttcc ggatcgttac gggtacacaa
    53461 ctcaccttcc ggtttgtaat aggccagaac gcgacaaatt tgttcagcgg actcacgtac
    53521 cgaaatcaga tgaccatcga tacgaatttt cagcccaggg gtaacttcaa cgcgatcgcc
    53581 gagtttagca attttgccat caacactcac acgaccggct tcaataatag attcgatttc
    53641 acggcgggat ccgtggccag cgcgggccag cactttctgt aatttttcgc tcatagagct
    53701 tccttttggt gtcgccttca caggcgtcga acaggagaat caatggcgcc gtatagcgcc
    53761 attttaaggc cgcgtagtat acagagttac gcacttataa gaaaggcttc acatcaccta
    53821 cgccttcacg caccacgacc ggcgtatcat cggtaagatc aataaccgtt gttggtttct
    53881 gcccgagata gccgccgtga ataatcaaat ctacctgttt ttccagacgg tctttaattt
    53941 cttcgggatc ggattcggta aattcgctgc ctggcagcat tagcgaagtg gaaagcatcg
    54001 gttcgcccag tgcttcaagc aacgcctgcg caatcgggtt agaaggcaca cgcataccga
    54061 tggttttgcg tttctcctgc aacagacggc gtggcacttc cttcgtcccc ttcaggatga
    54121 aggtatagtt gcccggcgtg ttgtttttca tcaaacgaaa cgcaacgtta tccacgaatg
    54181 aataggtcga gagttcagaa agatcgcgac acatcagggt aaagttgtga ccgtccggca
    54241 gctggcgaat acgacaaata cgctccatgg cgtttttatc ttcaatttta cagccgagcg
    54301 catagccgga atcagttgga tagacaatca ccccgccttt acgcacgatc tccaccgcct
    54361 ggttgatcag acgttgctgc gggttatcag gatgaatata aaaaaactgg ctcataattc
    54421 cctctctgtg gtgttctgcg gctgttccca tagctgccag acgccttcaa cgcctgcggg
    54481 cagccagagt ttacgaccca gttcgatcca cgggcatggc tgatgaaaat cagatccttg
    54541 tgatgcccat aaatgatgct gacgcgcaag ggccgccagc tgggtacgtt cattgggcga
    54601 ttgctggcac tgcgcgactt ccatcgcgtc accgtggtgt tcggcaaaat gcgcaaccaa
    54661 tcttttcagc catttagcac taagattgta ccgcccagga tgagctagca ccgccttacc
    54721 gccagaatga tgaatgacat caatagcttg ttctattgta caccactgtg gcggaacgta
    54781 tccggttttc ccgcgcgcca gatatttttt aaagacatcc gccattgaac tggctttgcc
    54841 gcaccccaca aggaaacgcg caaaatgacc gcgcgtcacc gcaccgccct gcgccagtcg
    54901 tcgcgcgcct tccagtgccc ctggtatttg cgctttttca agtctttcgg caatcagttg
    54961 cgcccgctga ttgcgccgtt ctgtctgctg cgcgaggaac tcacacatta gcggatgagt
    55021 aatatcaata ttcagcccga caatatgaat ttcatgattt tcccagaccg tggaaatttc
    55081 cacgccggga ataagattca gcgccagtcc cgaacgtgaa atttcctctc ttgctggcgc
    55141 gattgcagct gtggtgtcat ggtcggtgat cgccagggta ccgacgcgca tctcgactgc
    55201 acggtgcacc aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag
    55261 gtcgtaaatc actgcgtaat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt
    55321 tttgcgccga catcataacg gttctggcaa atattctgaa atcagctgtt gacaattaat
    55381 catcgaacta gttaactagt acgcaagttc acgtaaaaag ggtatcgaca atgaaagcaa
    55441 ttttcgtact gaaagcttgg tggcgcactt cctgaaacgg gcagtgtatt caccatgcgt
    55501 aaagcaatca gatacccagc ccgcctaatg agcgggcttt tttttgaaca aaattagaga
    55561 ataacaatgc aaacacaaaa accgactctc gaactgctaa cctgcgaagg cgcttatcgc
    55621 gacaacccga ctgcgctttt tcaccagttg tgtgggaatc gtccggcaac gctgctgctg
    55681 gaatccgcag atattgacag caaagatgat ttaaaaagcc tgctactggt agacagtgcg
    55741 ctgcgcatta cagcattagg tgacactgtc acaattcagg cactttccgg caacggcgaa
    55801 gccctgctgg cactactgga taacgccctg cctgcgggtg tggaaaatga acaatcacca
    55861 aactgccgtg tgctgcgctt cccccctgtc agtccactgc tggatgaaga cgcccgctta
    55921 tgctcccttt cggtttttga cgctttccgt ttattgcaga atctgttgaa tgtaccgaag
    55981 gaagaacgag aagcaatgtt cttcggcggc ctgttctctt atgaccttgt ggcgggattt
    56041 gaagatttac cgcaactgtc agcggaaaat aactgccctg atttctgttt ttatctcgct
    56101 gaaacgctga tggtgattga ccatcagaaa aaaaacaccc gcattcaggc cagcctgttt
    56161 gctccgaatg aagaagaaaa acaacgtctc actgctcgcc tgaacgatct tcgccagcag
    56221 ctgaccgaag ccgcgccgcc gctgccggtg gtttccgtgc cgcatatgcg ttgtgaatgt
    56281 aaccagagcg atgaagagtt cggtggcgta gtgcgtttgt tgcaaaaagc gattcgcgct
    56341 ggagaaattt tccaggtggt gccatctcgc cgtttctctc tgccctgccc gtcaccgctg
    56401 gcggcctatt acgtgctgaa aaagagtaat cccagcccgt acatgttttt tatgcaggat
    56461 aatgatttca ccctgtttgg cgcgtcgccg gaaagttcgc tcaagtatga cgccaccagc
    56521 cgccagattg agatctaccc gattgccgga acacgtccac gcggtcgtcg tgccgatggc
    56581 tcgctggaca gagacctcga cagccgcatc gaactggaaa tgcgtaccga tcataaagag
    56641 ctttctgaac atctgatgct ggtggatctc gcccgtaatg atctggcacg catttgcacc
    56701 cccggcagcc gctacgtcgc cgatcttacc aaagttgacc gttactctta cgtgatgcac
    56761 ctggtctccc gcgtggtcgg tgagctgcgc cacgatctcg acgccctgca cgcttaccgc
    56821 gcctgtatga atatggggac gttaagcggt gcgccgaaag tacgcgccat gcagttaatt
    56881 gccgaggcgg aaggtcgtcg ccgcggcagc tacggcggcg cggtaggtta tttcaccgcg
    56941 catggcgatc tcgacacctg cattgtgatc cgctcggcgc tggtggaaaa cggtatcgcc
    57001 accgtgcaag ccggtgctgg tgtagtcctt gattctgttc cgcagtcgga agccgacgaa
    57061 acccgtaata aagcccgcgc tgtactgcgc gctattgcca ccgcgcatca tgcacaggag
    57121 actttctgat ggctgacatt ctgctgctcg ataatatcga ctcttttacc tacaacctgg
    57181 cagatcagtt gcgcagcaat gggcataatg tggtgattta ccgcaaccat attccggcgc
    57241 agaccttaat tgaacgcctg gcgacgatga gcaatccggt actgatgctt tctcctggcc
    57301 ccggtgtgcc gagcgaagcc ggttgtatgc cggaactcct cacccgcttg cgtggcaagc
    57361 tgcccattat tggcatttgc ctcggacatc aggcaattgt cgaagcttac gggggctatg
    57421 tcggtcaggc gggcgaaatt cttcacggta aagcgtcgag cattgaacat gacggtcagg
    57481 cgatgtttgc cggattaaca aacccgctgc cggtggcgcg ttatcactcg ctggttggca
    57541 gtaacattcc ggccggttta accatcaacg cccattttaa tggcatggtg atggcagtac
    57601 gtcacgatgc ggatcgcgtt tgtggattcc agttccatcc ggaatccatt ctcaccaccc
    57661 agggcgctcg cctgctggaa caaacgctgg cctgggcgca gcagaaacta gagccagcca
    57721 acacgctgca accgattctg gaaaaactgt atcaggcgca gacgcttagc caacaagaaa
    57781 gccaccagct gttttcagcg gtggtgcgtg gcgagctgaa gccggaacaa ctggcggcgg
    57841 cgctggtgag catgaaaatt cgcggtgagc acccgaacga gatcgccggg gcagcaaccg
    57901 cgctactgga aaacgcagcg ccgttcccgc gcccggatta tctgtttgct gatatcgtcg
    57961 gtactggcgg tgacggcagc aacagtatca atatttctac cgccagtgcg tttgtcgccg
    58021 cggcctgtgg gctgaaagtg gcgaaacacg gcaaccgtag cgtctccagt aaatctggtt
    58081 cgtccgatct gctggcggcg ttcggtatta atcttgatat gaacgccgat aaatcgcgcc
    58141 aggcgctgga tgagttaggt gtttgtttcc tctttgcgcc gaagtatcac accggattcc
    58201 gccacgcgat gccggttcgc cagcaactga aaacccgcac cctgttcaat gtgctggggc
    58261 cattgattaa cccggcgcat ccgccgctgg cgttaattgg tgtttatagt ccggaactgg
    58321 tgctgccgat tgccgaaacc ttgcgcgtgc tggggtatca acgcgcggcg gtggtgcaca
    58381 gcggcgggat ggatgaagtt tcattacacg cgccgacaat cgttgccgaa ctgcatgacg
    58441 gtgaaattaa gagctatcag ctaactgcag aagattttgg cctgacaccc taccaccagg
    58501 agcaactggc aggcggaaca ccggaagaaa accgtgacat tttaacacgt ttgttacaag
    58561 gtaaaggcga cgccgcccat gaagcagccg tcgctgcgaa cgtcgccatg ttaatgcgcc
    58621 tgcatggcca tgaagatctg caagccaatg cgcaaaccgt tcttgaggta ctgcgcagtg
    58681 gttccgctta cgacagagtt accgcactgg cggcacgagg gtaaatgatg caaaccgttt
    58741 tagcgaaaat cgtcgcagac aaggcgattt gggtagaagc ccgcaaacag cagcaaccgc
    58801 tggccagttt tcagaatgag gttcagccga gcacgcgaca tttttatgat gcgctacagg
    58861 gtgcgcgcac ggcgtttatt ctggagtgca agaaagcgtc gccgtcaaaa ggcgtgatcc
    58921 gtgatgattt cgatccagca cgcattgccg ccatttataa acattacgct tcggcaattt
    58981 cggtgctgac tgatgagaaa tattttcagg ggagctttga tttcctcccc atcgtcagcc
    59041 aaatcgcccc gcagccgatt ttatgtaaag acttcattat cgacccttac cagatctatc
    59101 tggcgcgcta ttaccaggcc gatgcctgct tattaatgct ttcagtactg gatgacgacc
    59161 aatatcgcca gcttgcagcc gtcgctcaca gtctggagat gggggtgctg accgaagtca
    59221 gtaatgaaga ggaactggag cgcgccattg cattgggggc aaaggtcgtt ggcatcaaca
    59281 accgcgatct gcgtgatttg tcgattgatc tcaaccgtac ccgcgagctt gcgccgaaac
    59341 tgggacacaa cgtgacggta atcagcgaat ccggcatcaa tacttacgct caggtgcgcg
    59401 agttaagcca cttcgctaac ggttttctga ttggttcggc gttgatggcc catgacgatt
    59461 tgcacgccgc cgtgcgccgg gtgttgctgg gtgagaataa agtatgtggt ctgacgcgtg
    59521 ggcaagatgc taaagcagct tatgacgcgg gcgcgattta cggtgggttg attttggttg
    59581 cgacatcacc gcgttgcgtc aacgttgaac aggcgcagga agtgatggct gcggcaccgt
    59641 tgcagtatgt tggcgtgttc cgcaatcacg atattgccga tgtggtggac aaagctaagg
    59701 tgttatcgct ggcggcagtg caactgcatg gtaatgaaga tcagctgtat atcgatacgc
    59761 tgcgtgaagc tctgccagca cacgttgcca tctggaaagc attaagcgtc ggtgaaaccc
    59821 tgcccgcccg cgagcttcag cacgttgata aatatgtttt agacaacggc cagggtggaa
    59881 gcgggcaacg tttcgactgg tcactattaa atggtcaatc gcttggcaac gttctgctgg
    59941 cggggggctt aggcgcagat aactgcgtgg aagcggcaca aaccggctgc gccggacttg
    60001 attttaattc tgctgtagag tcgcaaccgg gcatcaaaga cgcacgtctt ttggcctcgg
    60061 ttttccagac gctgcgcgca tattaaggaa aggaacaatg acaacattac ttaaccccta
    60121 ttttggtgag tttggcggca tgtacgtgcc acaaatcctg atgcctgctc tgcgccagct
    60181 ggaagaagct tttgtcagcg cgcaaaaaga tcctgaattt caggcccagt tcaacgatct
    60241 actgaaaaac tatgccgggc gtccaaccgc actgaccaaa tgccagaaca ttacggctgg
    60301 cacgaacacc acgctgtatc tgaagcgcga agatttgctg cacggcggcg cgcataaaac
    60361 taaccaggtg ctcggtcagg ctttactggc gaagcggatg ggtaaaaccg aaatcatcgc
    60421 cgaaaccggt gccggtcagc atggcgtggc gtcggccctt gccagcgccc tgctcggcct
    60481 gaaatgccgt atttatatgg gtgccaaaga cgttgaacgc cagtcgccta acgtttttcg
    60541 tatgcgctta atgggtgcgg aagtgatccc ggtgcatagc ggttccgcga cgctgaaaga
    60601 tgcctgtaac gaggcgctgc gcgactggtc cggtagttac gaaaccgcgc actatatgct
    60661 gggcaccgca gctggcccgc atccttatcc gaccattgtg cgtgagtttc agcggatgat
    60721 tggcgaagaa accaaagcgc agattctgga aagagaaggt cgcctgccgg atgccgttat
    60781 cgcctgtgtt ggcggcggtt cgaatgccat cggcatgttt gctgatttca tcaatgaaac
    60841 caacgtcggc ctgattggtg tggagccagg tggtcacggt atcgaaactg gcgagcacgg
    60901 cgcaccgcta aaacatggtc gcgtgggtat ctatttcggt atgaaagcgc cgatgatgca
    60961 aaccgaagac gggcagattg aagaatctta ctccatctcc gccggattgg atttcccgtc
    61021 tgtcggccca caacacgcgt atcttaacag cactggacgc gctgattacg tgtctattac
    61081 cgatgatgaa gcccttgaag ccttcaagac gctgtgccta cacgaaggga tcatcccggc
    61141 gctggaatcc tcccacgccc tggcccatgc gctgaaaatg atgcgcgaaa acccggaaaa
    61201 agagcagcta ctggtggtta acctttccgg tcgcggcgat aaagacatct tcaccgttca
    61261 cgatattttg aaagcacgag gggaaatctg atggaacgct acgaatctct gtttgcccag
    61321 ttgaaggagc gcaaagaagg cgcattcgtt cctttcgtca cgctcgggga tccgggcatt
    61381 gagcagtcat tgaaaattat cgatacgcta attgaagccg gtgctgacgc gctggagtta
    61441 ggcatcccct tctccgaccc actggcagat ggcccgacga ttcaaaacgc cactctgcgc
    61501 gcctttgcgg caggtgtgac tccggcacaa tgttttgaaa tgctggcact gattcggcag
    61561 aaacacccga ccattcccat tggcctgttg atgtatgcca atctggtgtt taacaaaggc
    61621 attgatgagt tttatgccca gtgcgagaaa gtcggcgtcg attcggtgct ggttgccgat
    61681 gtgccaattg aagagtccgc gcccttccgc caggccgcgt tgcgccataa tgtcgcacct
    61741 atcttcatct gcccgccaaa tgccgatgac gacctgctgc gccagatagc ctcttacggt
    61801 cgtggttaca cctatttgct gtcacgagcg ggcgtgaccg gcgcagaaaa ccgcgccgcg
    61861 ttacccctca atcatctggt tgcgaagctg aaagagtaca acgctgcacc tccattgcag
    61921 gggtttggta tttccgcccc ggatcaggta aaagcagcga ttgatgcagg agctgcgggc
    61981 gcgatttctg gttcggccat tgttaaaatc atcgagcaac atattaatga gccagagaaa
    62041 atgctggcgg cactgaaagc ttttgtacaa ccgatgaaag cggcgacgcg cagttaatcc
    62101 cccagccgcc agttccgctg gcggcatttt aactttcttt aacgaagccg gaaaaatcct
    62161 aaattcattt aatatttatc tttttaccgt ttcgcttacc cctgtcgaac gtcaacttac
    62221 gtcatttttc cgcccaacag taatataatc aaacaaatta atcccgcaac ataacaccag
    62281 taaaatcaat aattttctct aagtcactta ttcctcaggc aattgttaat atatcaagaa
    62341 cgttcctcat aatatatttt cccactttct tctcgttacg cttaatttga ctaattctca
    62401 ttagcgacta attttaatga gtgtcgacac acaacactca tattaatgaa acaatgcaac
    62461 gcaacgggag aaataacatg gccgaacatc gtggtggttc aggaaatttc gccgaagacc
    62521 gtgagaaggc atccgacgca ggccgtaaag gcggtcagca tagcggcggt aattttaaaa
    62581 atgatccgca acgcgcatct gaagcgggta aaaaaggcgg tcaacaaagc ggtggtaata
    62641 aatcaggaaa atcctgattc accgttaatt atgaatatca gctgataaag ataattgcat
    62701 ctgcgggccg tatggctcgc agtgatttca ctggagaaaa atatgaatat gaagaccatt
    62761 gaagatgtat ttattcacct gctttcagat acctacagcg cagaaaaaca attaacccgg
    62821 gcactggcaa aactcgcaag agcaacatca aacgaaaaat taagtcaggc ttttcatgcg
    62881 cacctcgagg aaactcatgg acaaattgaa cgaattgatc aagttgtgga atctgaatca
    62941 aacctgaaaa ttaagcgcat gaaatgtgtg gcaatggaag gtctcattga agaagctaat
    63001 gaggtcatcg aaagcaccga gaaaaacgaa gtgcgtgatg ccgcactgat tgccgctgca
    63061 cagaaagtcg agcactatga gattgccagt tacgggactt tagcgacgct ggctgaacaa
    63121 ttaggttacc gtaaagcagc gaagcttctg aaagaaacgt tggaagaaga aaaggccacc
    63181 gacatcaaac tgactgatct ggcccttaat aacgtaaata agaaagccga aaataaagcc
    63241 tgaaatatga actttaactt ttagtcattt tataaagagg acattttcat gaatcgtatt
    63301 gaacattatc atgactggtt acgtgacgcc catgcaatgg aaaagcaagc cgaatctatg
    63361 cttgaatcca tggccagccg tatagataat tatcctgaac tacgcgctcg tattgaacaa
    63421 catcttagtg aaaccaaaaa ccagattgtt caactggaaa ctattcttga tcgtaatgac
    63481 atttcacgtt cagtcattaa agattccatg agtaaaatgg ctgcgcttgg gcagtcaatc
    63541 ggtggtatat tcccttctga tgaaatagtc aaaggctcta ttagcggata tgtcttcgag
    63601 caatttgaaa tcgcctgtta cacctctcta ttagcagcag caaaaaatgc cggtgataca
    63661 gcctcaattc cgatcatcga agcgatttta aatgaggaaa agcaaatggc cgactggctg
    63721 attcagcata ttccgcaaac aactgagaaa tttttaattc gctctgaaac tgatggcgta
    63781 gaagcgaaga aataataccc tttatgccat gtccttattg accccgtata ttacggggtc
    63841 gtttttgtgc ggaattaaaa acgatatcct gctgagaaca taaacaccca cggatcgagg
    63901 cgtacgctat catgttgctg tgcaccgccc agtttataat tggc
//
LOCUS       DAZKHC020000015        63025 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZKHC020000015 DAZKHC020000000
VERSION     DAZKHC020000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 63025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.015"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..663)
                     /gene="rpnC"
                     /locus_tag="ACHOSM_RS05755"
                     /old_locus_tag="pgaptmp_001151"
     CDS             complement(<1..663)
                     /gene="rpnC"
                     /locus_tag="ACHOSM_RS05755"
                     /old_locus_tag="pgaptmp_001151"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HGU4557721.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHT"
     gene            937..1317
                     /gene="panD"
                     /locus_tag="ACHOSM_RS05760"
                     /old_locus_tag="pgaptmp_001152"
     CDS             937..1317
                     /gene="panD"
                     /locus_tag="ACHOSM_RS05760"
                     /old_locus_tag="pgaptmp_001152"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HGU4557722.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(1321..2550)
                     /gene="yadE"
                     /locus_tag="ACHOSM_RS05765"
                     /old_locus_tag="pgaptmp_001153"
     CDS             complement(1321..2550)
                     /gene="yadE"
                     /locus_tag="ACHOSM_RS05765"
                     /old_locus_tag="pgaptmp_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGU4557723.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QYLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(2614..3054)
                     /gene="yadI"
                     /locus_tag="ACHOSM_RS05770"
                     /old_locus_tag="pgaptmp_001154"
     CDS             complement(2614..3054)
                     /gene="yadI"
                     /locus_tag="ACHOSM_RS05770"
                     /old_locus_tag="pgaptmp_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU4557724.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGVLLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(3159..3929)
                     /gene="yadH"
                     /locus_tag="ACHOSM_RS05775"
                     /old_locus_tag="pgaptmp_001155"
     CDS             complement(3159..3929)
                     /gene="yadH"
                     /locus_tag="ACHOSM_RS05775"
                     /old_locus_tag="pgaptmp_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4557725.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(3926..4852)
                     /gene="yadG"
                     /locus_tag="ACHOSM_RS05780"
                     /old_locus_tag="pgaptmp_001156"
     CDS             complement(3926..4852)
                     /gene="yadG"
                     /locus_tag="ACHOSM_RS05780"
                     /old_locus_tag="pgaptmp_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4557726.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            4961..5623
                     /gene="can"
                     /locus_tag="ACHOSM_RS05785"
                     /old_locus_tag="pgaptmp_001157"
     CDS             4961..5623
                     /gene="can"
                     /locus_tag="ACHOSM_RS05785"
                     /old_locus_tag="pgaptmp_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HGU4557727.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(5663..6199)
                     /gene="hpt"
                     /locus_tag="ACHOSM_RS05790"
                     /old_locus_tag="pgaptmp_001158"
     CDS             complement(5663..6199)
                     /gene="hpt"
                     /locus_tag="ACHOSM_RS05790"
                     /old_locus_tag="pgaptmp_001158"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGU4557728.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            6405..8795
                     /gene="gcd"
                     /locus_tag="ACHOSM_RS05795"
                     /old_locus_tag="pgaptmp_001159"
     CDS             6405..8795
                     /gene="gcd"
                     /locus_tag="ACHOSM_RS05795"
                     /old_locus_tag="pgaptmp_001159"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HGU4557729.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDTNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLTPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(8842..10392)
                     /gene="cueO"
                     /locus_tag="ACHOSM_RS05800"
                     /old_locus_tag="pgaptmp_001160"
     CDS             complement(8842..10392)
                     /gene="cueO"
                     /locus_tag="ACHOSM_RS05800"
                     /old_locus_tag="pgaptmp_001160"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HGU4557730.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISSVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            10558..10905
                     /gene="yacC"
                     /locus_tag="ACHOSM_RS05805"
                     /old_locus_tag="pgaptmp_001161"
     CDS             10558..10905
                     /gene="yacC"
                     /locus_tag="ACHOSM_RS05805"
                     /old_locus_tag="pgaptmp_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HGU4557731.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            11011..11877
                     /gene="speE"
                     /locus_tag="ACHOSM_RS05810"
                     /old_locus_tag="pgaptmp_001162"
     CDS             11011..11877
                     /gene="speE"
                     /locus_tag="ACHOSM_RS05810"
                     /old_locus_tag="pgaptmp_001162"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGU4557732.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            11893..12687
                     /gene="speD"
                     /locus_tag="ACHOSM_RS05815"
                     /old_locus_tag="pgaptmp_001163"
     CDS             11893..12687
                     /gene="speD"
                     /locus_tag="ACHOSM_RS05815"
                     /old_locus_tag="pgaptmp_001163"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HGU4557733.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(12724..13086)
                     /gene="yacL"
                     /locus_tag="ACHOSM_RS05820"
                     /old_locus_tag="pgaptmp_001164"
     CDS             complement(12724..13086)
                     /gene="yacL"
                     /locus_tag="ACHOSM_RS05820"
                     /old_locus_tag="pgaptmp_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HGU4557734.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(13261..15858)
                     /gene="acnB"
                     /locus_tag="ACHOSM_RS05825"
                     /old_locus_tag="pgaptmp_001165"
     CDS             complement(13261..15858)
                     /gene="acnB"
                     /locus_tag="ACHOSM_RS05825"
                     /old_locus_tag="pgaptmp_001165"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HGU4557735.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            16213..18066
                     /gene="yacH"
                     /locus_tag="ACHOSM_RS05830"
                     /old_locus_tag="pgaptmp_001166"
     CDS             16213..18066
                     /gene="yacH"
                     /locus_tag="ACHOSM_RS05830"
                     /old_locus_tag="pgaptmp_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HGU4557736.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSNEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPQIQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFRERVQESRPQRLNDSNRTARLNNDQRSAVRERLSERGARRLER"
     gene            complement(18137..19561)
                     /gene="lpdA"
                     /locus_tag="ACHOSM_RS05835"
                     /old_locus_tag="pgaptmp_001167"
     CDS             complement(18137..19561)
                     /gene="lpdA"
                     /locus_tag="ACHOSM_RS05835"
                     /old_locus_tag="pgaptmp_001167"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGU4557737.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(19886..21766)
                     /gene="aceF"
                     /locus_tag="ACHOSM_RS05840"
                     /old_locus_tag="pgaptmp_001168"
     CDS             complement(19886..21766)
                     /gene="aceF"
                     /locus_tag="ACHOSM_RS05840"
                     /old_locus_tag="pgaptmp_001168"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HGU4557738.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGINLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(21781..24444)
                     /gene="aceE"
                     /locus_tag="ACHOSM_RS05845"
                     /old_locus_tag="pgaptmp_001169"
     CDS             complement(21781..24444)
                     /gene="aceE"
                     /locus_tag="ACHOSM_RS05845"
                     /old_locus_tag="pgaptmp_001169"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HGU4557739.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTISFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(24605..25369)
                     /gene="pdhR"
                     /locus_tag="ACHOSM_RS05850"
                     /old_locus_tag="pgaptmp_001170"
     CDS             complement(24605..25369)
                     /gene="pdhR"
                     /locus_tag="ACHOSM_RS05850"
                     /old_locus_tag="pgaptmp_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HGU4557740.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            25912..27285
                     /gene="aroP"
                     /locus_tag="ACHOSM_RS05855"
                     /old_locus_tag="pgaptmp_001171"
     CDS             25912..27285
                     /gene="aroP"
                     /locus_tag="ACHOSM_RS05855"
                     /old_locus_tag="pgaptmp_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HGU4557741.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(27328..28182)
                     /gene="ampE"
                     /locus_tag="ACHOSM_RS05860"
                     /old_locus_tag="pgaptmp_001172"
     CDS             complement(27328..28182)
                     /gene="ampE"
                     /locus_tag="ACHOSM_RS05860"
                     /old_locus_tag="pgaptmp_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HGU4557742.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGLV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(28179..28730)
                     /gene="ampD"
                     /locus_tag="ACHOSM_RS05865"
                     /old_locus_tag="pgaptmp_001173"
     CDS             complement(28179..28730)
                     /gene="ampD"
                     /locus_tag="ACHOSM_RS05865"
                     /old_locus_tag="pgaptmp_001173"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HGU4557743.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            28818..29711
                     /gene="nadC"
                     /locus_tag="ACHOSM_RS05870"
                     /old_locus_tag="pgaptmp_001174"
     CDS             28818..29711
                     /gene="nadC"
                     /locus_tag="ACHOSM_RS05870"
                     /old_locus_tag="pgaptmp_001174"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGU4557744.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            29914..30354
                     /gene="ppdD"
                     /locus_tag="ACHOSM_RS05875"
                     /old_locus_tag="pgaptmp_001175"
     CDS             29914..30354
                     /gene="ppdD"
                     /locus_tag="ACHOSM_RS05875"
                     /old_locus_tag="pgaptmp_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HGU4557745.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            30364..31749
                     /gene="gspE"
                     /locus_tag="ACHOSM_RS05880"
                     /old_locus_tag="pgaptmp_001176"
     CDS             30364..31749
                     /gene="gspE"
                     /locus_tag="ACHOSM_RS05880"
                     /old_locus_tag="pgaptmp_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HGU4557746.1"
                     /translation="MNIPQLTALCLRYHGVLLNASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLLCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            31739..32941
                     /gene="hofC"
                     /locus_tag="ACHOSM_RS05885"
                     /old_locus_tag="pgaptmp_001177"
     CDS             31739..32941
                     /gene="hofC"
                     /locus_tag="ACHOSM_RS05885"
                     /old_locus_tag="pgaptmp_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HGU4557747.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(32976..34019)
                     /gene="guaC"
                     /locus_tag="ACHOSM_RS05890"
                     /old_locus_tag="pgaptmp_001178"
     CDS             complement(32976..34019)
                     /gene="guaC"
                     /locus_tag="ACHOSM_RS05890"
                     /old_locus_tag="pgaptmp_001178"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGU4557748.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(34175..34219)
                     /locus_tag="ACHOSM_RS05895"
                     /old_locus_tag="pgaptmp_001179"
     CDS             complement(34175..34219)
                     /locus_tag="ACHOSM_RS05895"
                     /old_locus_tag="pgaptmp_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557749.1"
                     /translation="MIIKTLKMSARKRN"
     gene            34244..34864
                     /gene="coaE"
                     /locus_tag="ACHOSM_RS05900"
                     /old_locus_tag="pgaptmp_001180"
     CDS             34244..34864
                     /gene="coaE"
                     /locus_tag="ACHOSM_RS05900"
                     /old_locus_tag="pgaptmp_001180"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGU4557750.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            34864..35607
                     /gene="zapD"
                     /locus_tag="ACHOSM_RS05905"
                     /old_locus_tag="pgaptmp_001181"
     CDS             34864..35607
                     /gene="zapD"
                     /locus_tag="ACHOSM_RS05905"
                     /old_locus_tag="pgaptmp_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HGU4557751.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            35617..35814
                     /gene="yacG"
                     /locus_tag="ACHOSM_RS05910"
                     /old_locus_tag="pgaptmp_001182"
     CDS             35617..35814
                     /gene="yacG"
                     /locus_tag="ACHOSM_RS05910"
                     /old_locus_tag="pgaptmp_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HGU4557752.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(35921..36310)
                     /gene="mutT"
                     /locus_tag="ACHOSM_RS05915"
                     /old_locus_tag="pgaptmp_001183"
     CDS             complement(35921..36310)
                     /gene="mutT"
                     /locus_tag="ACHOSM_RS05915"
                     /old_locus_tag="pgaptmp_001183"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HGU4557753.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLAYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(36370..39075)
                     /gene="secA"
                     /locus_tag="ACHOSM_RS05920"
                     /old_locus_tag="pgaptmp_001184"
     CDS             complement(36370..39075)
                     /gene="secA"
                     /locus_tag="ACHOSM_RS05920"
                     /old_locus_tag="pgaptmp_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGU4557754.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(39137..39649)
                     /gene="secM"
                     /locus_tag="ACHOSM_RS05925"
                     /old_locus_tag="pgaptmp_001185"
     CDS             complement(39137..39649)
                     /gene="secM"
                     /locus_tag="ACHOSM_RS05925"
                     /old_locus_tag="pgaptmp_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HGU4557755.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNVPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTQ
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(39880..40797)
                     /gene="lpxC"
                     /locus_tag="ACHOSM_RS05930"
                     /old_locus_tag="pgaptmp_001186"
     CDS             complement(39880..40797)
                     /gene="lpxC"
                     /locus_tag="ACHOSM_RS05930"
                     /old_locus_tag="pgaptmp_001186"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HGU4557756.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFIAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(40898..42049)
                     /gene="ftsZ"
                     /locus_tag="ACHOSM_RS05935"
                     /old_locus_tag="pgaptmp_001187"
     CDS             complement(40898..42049)
                     /gene="ftsZ"
                     /locus_tag="ACHOSM_RS05935"
                     /old_locus_tag="pgaptmp_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGU4557757.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(42110..43372)
                     /gene="ftsA"
                     /locus_tag="ACHOSM_RS05940"
                     /old_locus_tag="pgaptmp_001188"
     CDS             complement(42110..43372)
                     /gene="ftsA"
                     /locus_tag="ACHOSM_RS05940"
                     /old_locus_tag="pgaptmp_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGU4557758.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(43369..44199)
                     /gene="ftsQ"
                     /locus_tag="ACHOSM_RS05945"
                     /old_locus_tag="pgaptmp_001189"
     CDS             complement(43369..44199)
                     /gene="ftsQ"
                     /locus_tag="ACHOSM_RS05945"
                     /old_locus_tag="pgaptmp_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HGU4557759.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(44201..45121)
                     /gene="ddlB"
                     /locus_tag="ACHOSM_RS05950"
                     /old_locus_tag="pgaptmp_001190"
     CDS             complement(44201..45121)
                     /gene="ddlB"
                     /locus_tag="ACHOSM_RS05950"
                     /old_locus_tag="pgaptmp_001190"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU4557760.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPARTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(45114..46589)
                     /gene="murC"
                     /locus_tag="ACHOSM_RS05955"
                     /old_locus_tag="pgaptmp_001191"
     CDS             complement(45114..46589)
                     /gene="murC"
                     /locus_tag="ACHOSM_RS05955"
                     /old_locus_tag="pgaptmp_001191"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGU4557761.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(46643..47710)
                     /gene="murG"
                     /locus_tag="ACHOSM_RS05960"
                     /old_locus_tag="pgaptmp_001192"
     CDS             complement(46643..47710)
                     /gene="murG"
                     /locus_tag="ACHOSM_RS05960"
                     /old_locus_tag="pgaptmp_001192"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HGU4557762.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(47707..48951)
                     /gene="ftsW"
                     /locus_tag="ACHOSM_RS05965"
                     /old_locus_tag="pgaptmp_001193"
     CDS             complement(47707..48951)
                     /gene="ftsW"
                     /locus_tag="ACHOSM_RS05965"
                     /old_locus_tag="pgaptmp_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HGU4557763.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(48951..50267)
                     /gene="murD"
                     /locus_tag="ACHOSM_RS05970"
                     /old_locus_tag="pgaptmp_001194"
     CDS             complement(48951..50267)
                     /gene="murD"
                     /locus_tag="ACHOSM_RS05970"
                     /old_locus_tag="pgaptmp_001194"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGU4557764.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRLEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(50270..51352)
                     /gene="mraY"
                     /locus_tag="ACHOSM_RS05975"
                     /old_locus_tag="pgaptmp_001195"
     CDS             complement(50270..51352)
                     /gene="mraY"
                     /locus_tag="ACHOSM_RS05975"
                     /old_locus_tag="pgaptmp_001195"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGU4557765.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(51346..52704)
                     /gene="murF"
                     /locus_tag="ACHOSM_RS05980"
                     /old_locus_tag="pgaptmp_001196"
     CDS             complement(51346..52704)
                     /gene="murF"
                     /locus_tag="ACHOSM_RS05980"
                     /old_locus_tag="pgaptmp_001196"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGU4557766.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTGKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(52701..54188)
                     /gene="murE"
                     /locus_tag="ACHOSM_RS05985"
                     /old_locus_tag="pgaptmp_001197"
     CDS             complement(52701..54188)
                     /gene="murE"
                     /locus_tag="ACHOSM_RS05985"
                     /old_locus_tag="pgaptmp_001197"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HGU4557767.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     NDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTALGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(54175..55941)
                     /gene="ftsI"
                     /locus_tag="ACHOSM_RS05990"
                     /old_locus_tag="pgaptmp_001198"
     CDS             complement(54175..55941)
                     /gene="ftsI"
                     /locus_tag="ACHOSM_RS05990"
                     /old_locus_tag="pgaptmp_001198"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HGU4557768.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELKNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(55957..56322)
                     /gene="ftsL"
                     /locus_tag="ACHOSM_RS05995"
                     /old_locus_tag="pgaptmp_001199"
     CDS             complement(55957..56322)
                     /gene="ftsL"
                     /locus_tag="ACHOSM_RS05995"
                     /old_locus_tag="pgaptmp_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGU4557769.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(56319..57260)
                     /gene="rsmH"
                     /locus_tag="ACHOSM_RS06000"
                     /old_locus_tag="pgaptmp_001200"
     CDS             complement(56319..57260)
                     /gene="rsmH"
                     /locus_tag="ACHOSM_RS06000"
                     /old_locus_tag="pgaptmp_001200"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGU4557770.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(57262..57703)
                     /gene="mraZ"
                     /locus_tag="ACHOSM_RS06005"
                     /old_locus_tag="pgaptmp_001201"
                     /pseudo
     CDS             complement(57262..57703)
                     /gene="mraZ"
                     /locus_tag="ACHOSM_RS06005"
                     /old_locus_tag="pgaptmp_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
     gene            complement(58305..59309)
                     /gene="cra"
                     /locus_tag="ACHOSM_RS06010"
                     /old_locus_tag="pgaptmp_001202"
     CDS             complement(58305..59309)
                     /gene="cra"
                     /locus_tag="ACHOSM_RS06010"
                     /old_locus_tag="pgaptmp_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HGU4557771.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(59391..59477)
                     /locus_tag="ACHOSM_RS06015"
                     /old_locus_tag="pgaptmp_001203"
     CDS             complement(59391..59477)
                     /locus_tag="ACHOSM_RS06015"
                     /old_locus_tag="pgaptmp_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HGU4557772.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(59489..59980)
                     /gene="ilvN"
                     /locus_tag="ACHOSM_RS06020"
                     /old_locus_tag="pgaptmp_001204"
     CDS             complement(59489..59980)
                     /gene="ilvN"
                     /locus_tag="ACHOSM_RS06020"
                     /old_locus_tag="pgaptmp_001204"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU4557773.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(59983..61707)
                     /gene="ilvI"
                     /locus_tag="ACHOSM_RS06025"
                     /old_locus_tag="pgaptmp_001205"
     CDS             complement(59983..61707)
                     /gene="ilvI"
                     /locus_tag="ACHOSM_RS06025"
                     /old_locus_tag="pgaptmp_001205"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HGU4557774.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VVTSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            61826..61870
                     /locus_tag="ACHOSM_RS06030"
                     /old_locus_tag="pgaptmp_001206"
     CDS             61826..61870
                     /locus_tag="ACHOSM_RS06030"
                     /old_locus_tag="pgaptmp_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557775.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(62025..62968)
                     /gene="leuO"
                     /locus_tag="ACHOSM_RS06035"
                     /old_locus_tag="pgaptmp_001207"
                     /pseudo
     CDS             complement(62025..62968)
                     /gene="leuO"
                     /locus_tag="ACHOSM_RS06035"
                     /old_locus_tag="pgaptmp_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
BASE COUNT        15136 a        17449 c        15420 g        15020 t
ORIGIN      
        1 agtatgaccg cgttgcagca tatagttttg catggcaact aactgacttc cgctagtgta
       61 cccttcgtca atcagcgtga ccagttgctc cagcaataac attaagtcgc gctggcgaat
      121 atgtttttgc agtagttcga gaatcgcaat ccgccgatgt tgcatgattt cgtcatccgg
      181 tgtgatggtg atatccacca gcgggaaagg actgttatag acgcgtcgtg ccagctccgg
      241 cgagtaaaat atatcaaacc agcacattga gagcggataa ggtgtggcct cgccctgata
      301 aaacagtatc ggcaccacca gcggcagctt atcgtgatcc gcctccagat gccggtgcat
      361 ggcagcaata gaataacgca tcatgcgaaa ggccattttc ttatccggct tgctttggtg
      421 ttcaatcaca acatgcagat aaccgggatt gccctgcatt tgcacggaat agagcacgtc
      481 cgtgctgtgt cctttcaggc tctcttcaat gaaactcccc gactctaaat gaagcgtgtt
      541 gaggtcacaa agttctcgta attccactgg caaatgtatc tccagaaagt cgcgagccgt
      601 ctccgcgtgc attaaaaatt gtttaaacac tgcatcatgc ggtgtggtac tcggtgcatc
      661 catcttccct gccttccgtg ttgtcttgcg tgaaggcagt atccagtcaa ggcgtgccat
      721 cgtcagccac ctctttagtg tttaccgagc agcgttcaga gaaatttcaa ttggttacag
      781 ccgttacatg gcacgcttcg gcacggtacg caaactgctg atgtaaatgc cgtcagatga
      841 gtagacacta aacaaaaatc gggcaatact gcgtgagaat ttcctcaaag caggcatcgc
      901 ctgcttcgtt aacgacaggg tagaaaggta gaagttatga ttcgcacgat gctgcagggc
      961 aaactccacc gcgtgaaagt gactcatgcg gacctgcact atgaaggttc ttgcgccatt
     1021 gaccaggatt tccttgacgc agccggtatt ctcgaaaacg aagccattga tatctggaat
     1081 gtcaccaacg gcaagcgttt ctccacttat gccatcgcgg cagaacgcgg ttcgagaatt
     1141 atttctgtta acggtgcggc ggcccactgc gccagtgtcg gcgatattgt catcatcgcc
     1201 agcttcgtta ccatgccaga tgaagaagct cgcacctggc gacccaacgt cgcctatttt
     1261 gaaggcgaca atgaaatgaa acgtaccgcg aaagcgattc cggtacaggt tgcttgattg
     1321 ttatccctgc ggctggttac tcaccagccg cgacatcgtc tccagcgaat ccgttcttaa
     1381 gatataaagt cgttttagta acaacggatt atcccccggt tttactttgc ctttcatggt
     1441 tgtcaccgcc aggtgaaatc cggcatcgtt tgctgccttc acggctttgt cattaaatcc
     1501 gccaaacgga tacgaaagat accagacatg cggattaaat tgcgccagag cgcggcgtga
     1561 acgtgcaaaa tcaaacagaa tattgtgctc actacggctc agtaatatgg gtcggcgata
     1621 gccatccacc cgatgcaaaa aatgggtatg tgactgaaaa tcaaaaacat cgcgaatttc
     1681 gttaagctca gaaacgctca taaattgcag cgattttggg ttccacttct gcgggtgacg
     1741 tttgatgcgc gaggtaacaa caaacgccgt cgccttcatg ccatattgtt tcaatacagg
     1801 atacgcatag cggctcaccg acttgaggcc atcatcaaag gtaatcacca ccgctcgcgc
     1861 agggagattg atcttattct tcacgtaacc ttccagctgc gccatgctca gtgtcgcgta
     1921 tcccctgtcg cgcagccagg ccatttggtt attgaaagcg cgtaccgagg tggtcgtcga
     1981 agtatggcga aaacgggtgt tttcttcgtc gcgcagaata tggtgataag ttagcaccgg
     2041 aaggccatta tcgggttggg catccagcgc gctgatatag gccagtcgat cgccaatacg
     2101 gacctgatac caggtttgat ttaacctgtc tttcagttta tgcaaaatcg ggtagcgcaa
     2161 attgtccgcc aataccccaa atggcgcact gcccacactc ggcgcgttat agaccggcgt
     2221 atctttccag gtaactaagt actgattact cagcggcttg ttgaggtcac ccaaaccgtc
     2281 ttcaactttt tgtcgcccct gaaccggctc gagatgccct ttatcgataa accctttacc
     2341 aaagccaaaa ttaaatgcgt aataacttgc ggcagtgggc tccacggcaa tgatttgtcc
     2401 ggcccgaata ttccccacgg tcaccatctt gtcgccaatt tgcgcccaga ccgcagcatt
     2461 ttcgatggtt tgcatataac gggcaggtaa cgcggcactg acacttgcgg taaacagcat
     2521 cagcaggaga ataacagctt gtttgtacat gaaaaaataa ccgagggtga aacaacgctt
     2581 ttattgtagc aaaagcgcta taagaatcgt gggttaatac tcatacaaat tatgtttgag
     2641 gacgaaaggc ggattttttt gttgttggtg ccaaagacta ctcacctccg gcccacccag
     2701 ttcgacaata cgctcgcgaa actctgaact ggtcatggtt tcacggcaag ccatcatctg
     2761 ttcaattaac ggtaacgtga caccagaaat cacttcgcaa cgggagtgtt tgtggcttaa
     2821 taatgaagcc acgcggtacg gtggcgctcc ggctatatcg gttaagaaga tgacaccctc
     2881 accagagtcc gcttcatgca gagcatcgca catcatgcgg ctgagcatat tagagcttaa
     2941 tccgcgccag aaattcacgg cccggcactg aagaagtacc ccatgttttt tctccagcgc
     3001 atccagtatc tcttgcgccc ggtcatcgtg acaggtaatt acccaaccta acatcgcctt
     3061 cctccttagc cgaaaatcag tctaccctga ccattgttga taaagggtga tagccgtcaa
     3121 aactcctctc ccccaaatcc agaggagagg aaatagcctt agctacgcaa accacgtcca
     3181 cgttggatca gcgaccaaca gatcaaataa aacgccacaa taaagaccac cagtacgcca
     3241 aaggtagtga ccagcggaac atcattgata ccgaggaagc cgtagcggaa accactgatc
     3301 atataaacga ttgggttcag gtgcgacagc ccttgccaga acggcggcaa caaagtcagt
     3361 gagtaaaaga ccccacccaa atacgtgagt ggcgttaaca caaaggttgg caccaggctg
     3421 atgtcatcga acgttttggc aaacacaccg ttcagcaaac ccgcaaggga gaacaacacc
     3481 gccgtgagca ccagggttaa ggcaacgaat acccacgaat gcacctgaaa cggcacaaaa
     3541 aacagtgaaa ttgccgtcac cagaatgcca acaaacagac cacgcgccac gccaccgccg
     3601 acatatccgg caataatgac gtgagtcgga accggcgcta ccagcagctc ttcaatatta
     3661 cgctggaact tggcaccaaa aaatgatgac gcaacgttgg cgtaggcatt ggtgatcacc
     3721 gacatcatga tcagccccgg tacgatgaac tgcatatagc tgaagccatg catatcgcca
     3781 atacgcgaac caatcaggtt accgaagata ataaagtaaa gggtcatggt gatgactggc
     3841 ggcaccagcg tctgcaccca gatacgcata aagcgatgga tctctttcgc ccagatgctt
     3901 tttagcgcca cccagtaaag atgcatcatg cgcgatctcc ttgtttttca ttaaccagtg
     3961 aaacaaacag ctcttccaga cggttagctt tgttacgcat acttaatacc tgaatgccct
     4021 gctcacttaa ctgcgtaaat acgctgttga tcccctgctc acgcagcact tcaacttcca
     4081 gcgtcgcggt atcgaccagt cgatactgat agccatcgag cttcggtaac ggacttttcg
     4141 gtgcgagatc gagaataaag gtttccgatt tcagcttcgc cagcagcgcc ttcatcgagg
     4201 tattttccac cagctcaccg tgttgaataa tgccgatatt gcggcacagc atttctgctt
     4261 cttccaggta gtgtgtggtg agaatgatgg tggtgccttt gtcgtttaaa tccttcaaaa
     4321 agccccacat tgagcggcga agttcaatat ccacgcctgc ggtcggttcg tcgagaatca
     4381 gtagtttagg ttcatgcatt aacgcacggg caatcattaa acggcgcttc atcccgccag
     4441 ataacatacg cgcacgttcg ttgcgttttc cccatagatc gagttgttta agatattttt
     4501 cgctgcggat gtacgcctct ttgcgctcca caccgtagta ccctgcctga ttcaccacaa
     4561 tttgctgcac ggtttcaaac gggttgaagt taaattcctg cggcaccagt cccaactgac
     4621 gtttagcgtt cacgacatct ttctcgagat cgtaaccaaa tacgctgacc cgcccggagg
     4681 ttttatttac cagagagctg ataataccga tagtggtcga tttcccggcc ccgttcggcc
     4741 cgagaagcgc ataaaaatca cccgcttcga cctgcaaatc tattccacga agcgcctgaa
     4801 cgccgcctgg ataggttttt ttaagctgtt gaagttccag tgcaatggtc ataaattttt
     4861 acttacctta cgttcttaca ctttatctgt ggtttaaatc gtcccggagt tgccctatat
     4921 tagccaaacg taattatttg gttacaggtc gttaacctcc atgaaagaca tagatacact
     4981 catcagcaac aatgcactat ggtcaaaaat gctggtggaa gaggatcccg ggttttttga
     5041 gaaactggca caagcgcaaa aaccgcgctt tctatggatt ggatgttccg acagtcgcgt
     5101 tcctgcagaa cgtttaaccg gtcttgagcc gggcgaactc tttgttcacc gtaatgttgc
     5161 taacctggtc attcacaccg acctgaactg cctttccgtg gttcagtatg cagtggatgt
     5221 actcgaagtt gaacacatta ttatctgtgg ccactacggt tgcggcggcg tacaagccgc
     5281 agttgaaaac ccggaactgg ggcttatcaa caactggctg ctgcatatcc gcgatatctg
     5341 gttcaaacat agctcattgc tcggcgaaat gccgcaagag cgccgtctgg ataccttgtg
     5401 tgaactgaac gtcatggaac aggtttataa cctgggccac tccaccatta tgcaatcagc
     5461 gtggaaacgc gggcagaaag ttaccattca cggctgggcc tacggcattc acgacggctt
     5521 gctgcgtgat ctggatgtca cagcgaccaa ccgcgaaacc cttgagcaac gttaccgtca
     5581 cgggatttcc aacctcaagc tgaaacacgc caaccacaaa taaaatgcca tgccggatgc
     5641 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgccg atatacggca
     5701 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
     5761 agaaaccgat aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg
     5821 tacaaatcgc caacgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg
     5881 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
     5941 ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
     6001 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
     6061 taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct
     6121 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
     6181 cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg
     6241 gcaggttttg gcaccgcaac aaagcgcaag acacgcagta taccagtaaa actattcatt
     6301 cgcggaatct gttaataaag ctcgaattgt gatgacggtc acacatgtta aaacctataa
     6361 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
     6421 caggctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
     6481 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
     6541 ctggccttgt gatgctcggc gtcgcctgga tgctgtggtg cagtaaacgc gccgcgcttt
     6601 ggctatacgc agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg
     6661 acttctgggc gctgactccg cgcagcgata ttctggtctt cttcggcatc tggctgatcc
     6721 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
     6781 tggcactgct gattagcggt agtatcctga cctgggccgg atttaacgat ccgcaggaaa
     6841 tcaacggcac cttaagcgcc gatgccacac ctgcggaagc tatctcaccc gttgccgatc
     6901 aggactggcc tgcctatggt cgcaatcagg aaggtcaacg cttttcacct ctgaaacaaa
     6961 ttaacgccga taacgtccat aatctgaaag aagcctgggt gttccgtact ggcgatgtga
     7021 agcagccgaa cgatccgggc gaaatcacca atgaagtgac gccgattaaa gtgggcgaca
     7081 ccctttacct gtgtaccgct caccagcgcc tgtttgcgct cgatgccgcc agcggcaaag
     7141 agaaatggca ttacgatcct gagctgaaaa ctaacgagtc tttccagcac gtaacctgcc
     7201 gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt
     7261 gcccgcgtcg tatcattctt ccggtcaatg atggtcgact gattgcgatt aacgctgaaa
     7321 acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
     7381 cagacaccaa accgggtctg tatgaaccga cttcgccacc gattatcacc gataaaacca
     7441 tcgtgatggc cggttcagtt accgataact tctcaacccg cgaaacgtct ggcgtgatcc
     7501 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatcccggc gcgaaagata
     7561 cgaacgcaat cccgtctgac gaacacacct ttacctttaa ctcgccaaat tcctgggcac
     7621 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg
     7681 atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc
     7741 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
     7801 acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
     7861 cagttattta cgctccggcg aaaaccggca acatttttgt gctcgatcgt cgcaatggcg
     7921 aactggtggt tccggccccg gaaaaaccgg tgccgcaagg tgctgcgaaa ggcgattacg
     7981 ttactccgac ccagccgttc tctgaactga gcttccgtcc gaagaaagat ttgagcggtg
     8041 cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga
     8101 tgcgctatga aggcattttc acgccgccat ctgaacaggg cacgctggtc ttcccgggta
     8161 acctgggaat gtttgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg
     8221 ccaacccaat ggcactgccg tttgtttcga aactgatccc acgcggtccg ggtaacccga
     8281 tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg
     8341 gtgtaccgta tggtgtcacg ctcaccccgt tcctctcacc gtttggtctg ccgtgtaaac
     8401 agccagcatg gggttatatc tcggcgctgg atctgaaaac caatgaagtg gtgtggaaga
     8461 aacgtattgg tacgccgcaa gacagtatgc cgttcccgat gcctgtaccg gtgccgttca
     8521 atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
     8581 ctgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
     8641 agggccgttt accagcgggt ggtcaggcga cgccaatgac ctatgaagtg aatggcaagc
     8701 agtatgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
     8761 ttgtggctta tgccctgccg gatgatgtga aataaccata atgcccggag agatccgggc
     8821 atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg
     8881 ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt
     8941 caccagcact tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg
     9001 agccgctggc ggtttgccat tttctgacaa gatacggaac tgcgtgccgt gaatatggaa
     9061 cggatgcagc atcatgtcgc ccacgctaga gataacccaa cgttcgtact gccctttcgc
     9121 cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg
     9181 gaaatcgaac ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat
     9241 catctggcta tgatccatcc cggccatcgc ctgatcgcca tatttctcca ttagcatctg
     9301 catacccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc
     9361 ttccagcgaa ggtaacgcag gcaggctact taatgtgtct ggcaaagcac cggaggcact
     9421 aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggtg caatcgccat
     9481 ccccatctgg ctgaccggca gagtcaccag gtcaaagggt ttgttgtcgt taacctccac
     9541 cagcacttca aaacgctcgc ccatcagcac cggcagctcg ttcaccttca ccggttcagg
     9601 tagcagacca ccgtcgctgg caatcacata aagcgggcga ttgtcgctgg tggcgaaatt
     9661 gagcgagcgg gcattacagc cattgagcaa acgtaggcgc agccagccac gcggggcagc
     9721 gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc
     9781 ggtcatcaca tccagttgat aatcaatctg cccgtcagcg ctaaattttt tatcctgaac
     9841 aatcaccgga acatcatcaa taccccactg ttttggcagc attaatttca ggatctcgtc
     9901 atcttcaatt accaccagac cagccagccc catcgccacc tgtcgcccgg tcttgccatg
     9961 ttgatgtgga tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg
    10021 cttgccacct ggcggaataa ttccctgggg gccgccgtcc acttcacccg gtacttccag
    10081 cccgtgccag tgcaacgtcg tctcttccgt cagttggtta tagatatcaa ccgttaccgc
    10141 tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt
    10201 tgcagttttc tcgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg
    10261 ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaatactgc
    10321 gcggctccac agcggcaagg ctgaagccac acccagcgcg acggaatatt ttaagaaatc
    10381 acggcgttgc atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc
    10441 atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt
    10501 cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg
    10561 aagacgtttt tcagaacagt gttattcggc agcctgatgg ccgtttgcgc aaacagttac
    10621 gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg
    10681 aagaacgatt gtggttacca gaacttacct aacgggcaaa ttcgtcgcgc actggtcttt
    10741 ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagctctc
    10801 ggtgaagaca gctaccgcga tctcagcggc attggcattc ctgtcgctaa aaaatgcaaa
    10861 gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt
    10921 tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt
    10981 tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg
    11041 ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc
    11101 gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat
    11161 ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
    11221 ccgctactgg cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc
    11281 atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat
    11341 gcgggtgtcg tatcgttctg ccgtcagtat ctacccaacc ataacgccgg tagctacgac
    11401 gatccgcgct ttaagctggt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag
    11461 acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga aagccttttc
    11521 acttcggcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca
    11581 caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc
    11641 cattacttca gcgacgttgg cttttatcag gcggcgattc cgacctatta cggcggtatc
    11701 atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt
    11761 caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg
    11821 gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga
    11881 gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg
    11941 agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt
    12001 gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt
    12061 tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc
    12121 agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa
    12181 caccccggcc cactgccaga aacggtcgtt gcccatctcg ataaaagtca tatttgcgta
    12241 catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa
    12301 gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt
    12361 gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttacccgcga cattaacggt
    12421 atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg
    12481 aaggcgctgt atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag
    12541 atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta
    12601 accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat
    12661 tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctccaggct ccgccagatt
    12721 tatttacttc tgctgcacga aattgcggta agccgccacg acctgcagaa aatcctcaac
    12781 gccgcatagc gacaggcttt cttcgtcgta gtagttcatc ccctcttcca tttcatcgcc
    12841 agcgaattcc agttgattgg cgcgaaccat cacctcttca ccgtccatcc acagggtgta
    12901 ttcatgccct gcccgttgcc aggaccgttc gctacctttc agtgcgtgcg ctgcttgttc
    12961 cacttcatca agcaaggcca ggttttcttt cacctcttca ttaaaccagt gcccgaccac
    13021 ttcatgcccc atggacatac gcacctttac cactccggta atatcgcgca gaaattcgta
    13081 atccatagtg tgttcctctg ctcccggcaa tacgccgaaa tcgcattgcg ccattgatgt
    13141 aattatcgca gccttaccgg agaaaaacag cggaacggcg atggtttaga aataaaaaat
    13201 gcccgggaat ctcattcacc gggcattgtg tcgtttatgc gcagcgcgtg cgcttactta
    13261 ttacaccgca gtctggaaaa tcaccccatc ggctttttcg gtgtactgag aaagttggtt
    13321 gaagttcaga taacgataag tatcaacggc agttttatct acttgcgcaa cgtaggtctg
    13381 gtactcttcc ggcgtcggca gtttgccaat cagcgccgca acagccgcca gttctgcaga
    13441 agccaggaag acattcgcgc cagtccccag acggttcggg aagttacggg tagaggtgga
    13501 aaccaccgtc gcaccgtctg ctacacgcgc ctggttaccc atacacagag aacagcccgg
    13561 gatctcgata cgagccccgc tcttaccgaa gacgctgtag tagccttctt cagtcaactg
    13621 tgcggcgtcc atacgggttg gcggtgccac ccacaggcgg gtcggcaact ggcctttatg
    13681 cgcatccagc agtttacccg cagcacggaa gtgaccgatg ttagtcatgc aagaaccgat
    13741 aaatacttcg tcgatcttct cgccctgtac cgcagacagc ggacgcgcgt catccgggtc
    13801 gttcggagca cacaggattg gctctttaat atccgccaga tcgatgtcaa tcactgccgc
    13861 gtattccgca tctgcatcgg cttccagcag ctcaggattc gccagccatt tttccatgcc
    13921 ctgaatacga cgttccaggg tacgacgatc gccgtaacct tccgcgatca tccacttcag
    13981 caggacgata ttagagttaa ggtattcgat gattggttct ttgttcagct tgatagtaca
    14041 accagcggca gaacgctcag cggacgcatc ggtaagctca aaggcctgct caactttcag
    14101 atccggcaga ccttcaattt ccagaatgcg gccagagaag atgtttttct tgcctttctt
    14161 ctcaacggtc agcagacctt gtttgatggc atacagcgga atggcgtgta ccagatcgcg
    14221 cagggtgatg cccggctgca ttttgccttt gaagcgcacc agaacggatt ccggcatatc
    14281 cagcggcatt acgcctgttg cggctgcaaa tgccaccaga ccagaacccg ccgggaaaga
    14341 gataccgatc gggaaacggg tatgggagtc acctccggta ccgacggtat ccggcagcag
    14401 catacggttc agccaggagt ggattacgcc gtcacccgga cgcagcgaca caccgccacg
    14461 gttcataatg aagtccggca gcgtgtggtg cgtgttcacg tcaactggct tcggatacgc
    14521 cgcggtgtgg cagaaagact gcatcaccag gtcagccgag aagcccaggc acgccaggtc
    14581 tttcagttca tcacgggtca tcgggccggt ggtgtcctgt gaacctacag aagtcatttt
    14641 cggttcgcag tacgcgcccg gacgaatgcc tttcacgcca caggcacggc ctaccatttt
    14701 ctgcgccagc gagaagccgc gatcgctctc agcgacatct ttcgcctgac ggaacacatc
    14761 actgtgcggc agaccaagtg cttcacgcgc tttggtggtc aggccacgcc cgataatcag
    14821 cgggatacgg ccgccagcac gcacttcatc aatcagcacg tcggttttca gttcgaaggt
    14881 cgccagcagt tcgcctgttt cgtggttacg cacttcacct ttgtacgggt aaacgtcaat
    14941 cacgtcgccc atgttcaggt tagagacgtc gacttcgatt ggcagtgcac ccgcgtcttc
    15001 catcgtgtta aagaagatgg gtgcaatttt accgccgagg cacaaaccac cgccgcgttt
    15061 gttcggcaca tgtggaatat catcgcccat aaaccacaga acggagttcg tggcggattt
    15121 acgcgatgaa cccgtaccca caacgtcacc gacgtacgcc agcgggaaac ctttctgttg
    15181 cagagcttca atctgtttga tcgggccaac aacacctggc tggtctggct caataccttc
    15241 acgggcgttt ttcagcatcg ccagcgcgtg cagtgggata tccgggcgcg accacgcatc
    15301 cggtgccgga gaaaggtcat cggtgttggt ttcgccagtg actttgaaga cggtaacggt
    15361 cagtttttca gccagcgccg ggcgattcag gaaccattcg gcatccgccc aggactgcat
    15421 tacctgcttc gcatattcgt tgcctgcttt cgctttctct tctacgtcat agaagttatc
    15481 gaacatcagc agtgtgtgag aaagtgcttt ggcagcgatc ggtgccagtt tggcatcatc
    15541 cagcgcgtcg atcagcggat gaatgttgta accaccctgc atggtgccca gcagttcgat
    15601 ggctttttcc ggagtcagca gtggggattt ggcttcacct ttcgcgacag cagccaggaa
    15661 gcctgctttg acataggcgg cttcatcgac gcctggggga acacggttgg ttaatagatc
    15721 taacaggaat tcttcttcgc ccgcgggcgg gtttttcagc agctctacaa gtgcggccat
    15781 ttggtttgca tccaggggtt tgggcgcaat cccctcagcg gcacgctcag ctacgtgctt
    15841 acggtattct tctagcacga cggttctcct cgctctcatt gtcatagtgc ggcaggcgat
    15901 tctcttcacg ctcctgtgag acagcagttt gtagggtaaa tgcccagtac cgcaacgggc
    15961 agaatagcag gattttgacg acgtgttaat ctgtttacaa aaaagcaaca taaaaaagtg
    16021 gctgaatcgt taaggatggt ctagagattg tttccctccg taaaaacttc gttaattcgc
    16081 atggtgataa tcacctttct caacgaatcc caagattagt caaaatttaa actaccgcct
    16141 ctttatactc ggattaacag cacctgcggg tggcagttcg ccgaccattg cgatttcctt
    16201 gagatccgaa ttatgaaaat gactttgccg tttaaacccc atgtgctggc actaatttgc
    16261 agtgccgggc tttgtgccgc ctctgccggg ctatatataa aaagccgcac agtggaagcg
    16321 cctgtggaaa cgcaatcgac acaactggct gtgtctgacg ctgccgcggt tacgcttcct
    16381 gcaacggttt ccgcgcctcc cgtaacaccc gccgtcgtta aatccgcatt cagcactgca
    16441 caaatagatc aatgggtcgc gcccgtcgcg ctgtatcccg acgccctact ttcgcaggtg
    16501 ctgatggcat caacctatcc ggcaaacgtt gctcaagcag tgcaatggtc gcacgataat
    16561 ccacttaaac aaggcgatgc tgctattcag gcggtatctg accagccgtg ggacgccagc
    16621 gttaaatcac tggtggcctt tccacaattg atggcattga tgggcgaaaa cccgcaatgg
    16681 gtgcaaaacc tgggcgatgc ttttctggcg cagccgcagg acgtgatgga ctcggtacag
    16741 cgattgcggc aactggcgca acaaaccggt tcgctgaagt catcaaacga acaaaaagtt
    16801 attaccacaa cgaagaaagc tgtaccggta aaacagacag tcacggcacc cgtcatacca
    16861 tccaataccg ttttaactgc cagccccgtc attacagagc ctgcaacaac cgtcatttcc
    16921 attgagcccg ccaatcctga tgtggtctat attcccaact acaacccaac cgtggtttac
    16981 gggaactggg ccaatactgc gtatccgccg gtttatctgc caccaccagc cggagaaccg
    17041 tttattgaca gctttgtgcg cggattcggc tatagcatgg gtgttgctac cacgtacgca
    17101 ctattcagca gcatcgactg ggatgacgac gatcatgacc atcatcatca tgacgatgat
    17161 gattatcatc accacgatgg cggtcatcgt gacggtaatg gctggcaaca caacggcgac
    17221 aacatcaata tcgacgtcaa caatttcaac cgtatcaccg gtgagcatct tactgataag
    17281 aatatggcat ggcggcacaa tccaaactac cgtaatggtg tgccctatca tgatcaggat
    17341 atggcaaagc ggtttcatca aaccgatgtc aacggcggaa tgagtgccac gcagctacct
    17401 gctccaacac gcgacagcca gcgtcaggcg gcagcaagtc agtttcagca acgaacacac
    17461 gccgcccccg tcattacacg agatacccaa cgtcaggcag cggcacagcg gtttaatgaa
    17521 gctgaacact atgggagcta tgacgacttc cgcgacttca gccgtcgcca acccctgacc
    17581 cagcaacaaa aggacgccgc tcgtcagcgt tatcagtcag cttctcctga gcagcgccag
    17641 gcagttcgcg agagaatgca gactaacccg cagatccagc agcgaagaga ggcagcgcgt
    17701 gagcgcattc agcccgcctc gcctgagcag cgccaggcag tccgcgagaa aatgcagact
    17761 aacccacaga tccagcagcg aagagacgca gcgcgtgagc gtattcagtc agcctcgcct
    17821 gagcagcgcc aggtgtttaa ggaaaaagta cagcagcgcc cactgaacca acagcaacgt
    17881 gataacgccc gccagcgtgt tcaatcagca tcacctgaac aacgtcaggt ttttcgggag
    17941 agagttcagg agagccgccc acaacgtcta aacgacagta accgtactgc cagattgaat
    18001 aacgatcaac ggtcagcagt acgcgaacgt ctctctgagc gcggagcaag gcgactggaa
    18061 aggtaaatta cagacgtaaa aaaagcggcg tggttagccg cttttttaat tgccggatgt
    18121 tccggcaaac gaaaaattac ttcttcttcg ctttcgggtt cggcaggtcg gtaatgctac
    18181 cttcgaacac ttctgccgcc aggcccacag actcgtgcag agtcgggtgc gcatggatgg
    18241 tcagcgcgat gtcttcagcg tcacaaccca tttcgattgc caggccgatt tcacccagca
    18301 gctcgccgcc gttggtaccg acaatcgcac caccgatcac acggtgagat tctttgtcga
    18361 aaatcagctt ggtcataccg tctgcgcagt cggaagcgat agcacgacca gaagcagccc
    18421 acgggaaggt ggcggtttca tagctgatgc ctttctcttt cgcttctttc tcagtcagac
    18481 ctacccatgc aacttctggt tcggtatagg cgatagacgg gataactttc ggatcgaagt
    18541 agtgtttttt accggcgata acttcagcgg caacgtgacc ttcgtgaaca cctttgtgtg
    18601 ccagcatcgg ctgaccaacg atatcgccga tagcaaagat gtgcggtacg ttggtacgca
    18661 gctgtttgtc aacgcggatg aaaccacggt cgtccacttc cacgccagct ttgcctgcgt
    18721 cgaggttttt accgttcggc acacgaccaa tcgctaccag cacggcgtcg taacgctgcg
    18781 gttcagcggg tgcttttttg ccttccatcg tcacataaat accgtcttct ttcgcttcaa
    18841 cggcggtaac tttggtttcc agcatcaggt tgaatttctt gctgatacgc ttggtgaaga
    18901 ctttaacgat gtctttgtca gctgccggga taacctggtc gaacatttca accacgtcaa
    18961 tctgtgaacc cagcgcgtgg tatacggtgc ccatttccag accgatgata ccgccaccca
    19021 ttaccagcag gcgttctggt acttctttca gttccagcgc gtcagtggag tcccagatac
    19081 gcggatcttc atgcggaata aacggcagtt ggatcgggcg agaacccgct gcaatgatcg
    19141 cattgtcgaa gttgatcacg gttttgccgt tctcaccttc aacttccagg gtgttagccc
    19201 cggtgaattt acccagaccg ttgaccactt tgactttgcg gcctttcgcc ataccagcca
    19261 gaccaccggt cagctgattg atcactttct ctttccaggt acgaatcttg tcaatatcgg
    19321 ttttcggttc gccgaagacg ataccgtgtt cagccagcgc tttggcttct tcgataactt
    19381 ttgctacgtg cagcagtgct ttagaaggga tacagccgac gttcaggcaa acaccgccaa
    19441 gggtgttgta acgttctacg attacggttt ccagacctaa atcagcgcaa cggaaggcag
    19501 cggagtaacc tgcggggcct gccccaagta ccacgacctg agttttgatt tcagtactca
    19561 tcatgacctc tatatattta tctccggcgg tcatacccgt cgtctttcag gccgcagcgg
    19621 cgttagcttc acgccctcac cccagtcact tacttatgta agctcctggg gattctggcg
    19681 cttgctacct tgctgcaccc agaaatccat agggtatgtg aagtaatacc ctaaccacca
    19741 ccgggtcgtt ctatccgtcg gtattttaca aaattgttaa caatttttaa acaacaaacg
    19801 gcaaccgatt tgtctattcg ctaataacct caatacattc atgagattac cagaaaaaag
    19861 ccggccgttg ggccggctct tttacttaca tcaccagacg gcgaatgtca gacagcgtgt
    19921 tgttaatgat ggtaatgaaa cgggcaccat cagcaccgtc gatcacgcgg tggtcgaagg
    19981 agagagaaat cggcagcatc agacgcggca cgaactcttt accattccac accggctcca
    20041 tcgcggactt ggaaacgccg aggatagcca cttccggcgc gttcacaatc ggcgcgaagt
    20101 gggtagtacc caggccgccg atgctggaga tggtgaagca accgccctgc atttcgcccg
    20161 cagtcagctt accgtcacgc gctttcttag aaatagtcat cagctcgcga gacagctcga
    20221 tgatgccttt cttgttgacg tctttgaata ccggaacaac cagaccgttc ggggtatcca
    20281 ccgccacacc gatgttgatg tatttcttca gggtcagacg ctgaccgtct tccgacagcg
    20341 aactattgaa gcgaggcatc tgctcaagag ctgcagcaac ggctttcatg atgaagacaa
    20401 ccggggtgat cttcacatcc agcttacgtt tcgccgcttc ttcgttctgc tgtttacgga
    20461 acgcttccag ctcggtgata tcggttttgt cgaagtgagt aacatgcggg atcatcaccc
    20521 agttacggct caggttcgca ccagaaattt tctggatacg gcccagttcc acttcttcga
    20581 tttcaccaaa cttgctgaag tccaccttcg gccacggcag catgcctggg ataccgccgc
    20641 cagtcgccgc cggagctgct tctgcacgtt tgatagcttc tttcacgtaa gcctgaacgt
    20701 cttcgcgcag gatacgacct ttacggccag tgcccttcac tttcgccagg ttaatgccaa
    20761 actcgcgtgc cagacggcgg atcagcggag tcgcgtgaac ataagcgtcg ttttcagcaa
    20821 attcagattt gccttccgct ttcgcagctg gtgctgctgc cggggcttca gcttttgctg
    20881 ccggtgccgg cgctgccgct tcctgtttcg caggcgctgc gccttcaact tcgaagatca
    20941 taatcagcga gccagttttc actttatcgc caacgttgac tttcagttcc ttcacgacgc
    21001 ctgcaaacgg cgccggaact tccatagaag ctttgtcgcc ttctacggtg atcagtgact
    21061 gttcagcggc aactttgtcg cccactttca ccatcacttc agtcacttca acttcgtcac
    21121 cgccgatatc cggaacgtta acttctttca cgccagctgc tggtgcaggg gccgctgccg
    21181 gagccgcttc ctgtttagcc gccggagctg ccgcgcctgc ttcacccgcg acttcgaaga
    21241 ccataatcag cgagccggta gacactttgt cacccacgtt cactttgatc tctttcacgg
    21301 tgccagcaaa cggagccgga acttccatag aagctttatc gccttctacg gtgatcagcg
    21361 actgttcagc ttcaacttta tcgccaactt tcaccaggat ttcggtcact tcaacttcgt
    21421 cgctgccgat atccggaacg ttaacgtctt ttgccgccgc agccgcgggt gctgctgccg
    21481 gagctgcttc tttcttctct tctgcctgag caggtgcagc gtctgctgca ccgtcggcgg
    21541 aatcgaaaat cataatcagt gcgccggtct gggttttatc gccaacagag actttgatct
    21601 ctttaacgat acccgcctgc ggagacggaa cttccataga ggctttgtcg ccttctacgg
    21661 tgatcagcga ctgttcggct tcaactttgt cgcccacttt gaccaggatc tcggtgattt
    21721 caacttcatc agccccgatg tccggtactt tgatttcgat agccattatt cttttacctc
    21781 ttacgccaga cgcgggttaa ctttatctgc atcgatgttg aatttggcga ttgcgtcagc
    21841 aaccactttc ttatcgattt cgccacgttt agccagttcg cccagcgccg caaccacgac
    21901 ataagaagca tcaacttcga agtggtgacg caggttctca cggctgtcgg aacgaccgaa
    21961 gccatcagta cccagtacgc ggtagtcgtc agccggtacg taagtacgga cctgctcagc
    22021 gaacagtttc atatagtcgg tagatgccac tgccggagcg tcgttcatca cctgagcgat
    22081 atacggaacg cgcggagttt ccagcggatg aagcatgttc cagcgttcac aatcctgacc
    22141 atcacgcgcc agctcggtga aggaggtcac gctataaacg tcagaaccta caccgtaatc
    22201 tttcgccaag atctctgccg cttcacggac gtgacgcagg atagaaccgg agcccagcag
    22261 ctgaacttta cctttgctac cttcaatggt ttcgagtttg tagatacctt tacggatacc
    22321 ttcctcagca ccttccggca ttgccggcat gtggtagttt tcgttcagcg tggtgatgta
    22381 gtagtaaacg ttctcttgtt tttcaccgta catacgctcc agaccgtcat gcataatgac
    22441 agcaacttcg taagcgtaag ccgggtcgta agagatacag ttcggaatag tcagcgactg
    22501 aatgtggctg tgaccatctt cgtgctgcag accttcgccg ttcagggtgg tacgaccgga
    22561 agtaccgccg atcaggaagc cacgcgcttg ctggtcgcca gccgcccagc acagatcgcc
    22621 aatacgctgg aagccgaaca tcgagtaata gatgtagaac gggatcatcg gcagattgtt
    22681 ggtgctgtaa gaggtcgccg ctgccagcca ggaacaacct gcgcccagct cgttgatccc
    22741 ttcctgcaga atctgacctt tctcgtcttc tttatagtaa gcaacctgct cgcggtcctg
    22801 cggggtgtac tgctgaccgt tcgggctgta aataccaatc tgacggaaca gaccttccat
    22861 accgaaagta cgcgcttcgt cggcgatgat cggtaccaga cgatctttga tcgacttgtt
    22921 cttcagcatt acgttcaggg cacgaacgaa agagatagtg gtagagatct ctttgctctg
    22981 ctcttccagc agcgcgccga agtcttgcag gctcggcagc tcaagcttct cggtgaagtt
    23041 cggctgacgg cttggcagat aaccgtgcag tttctgacgc tgtgcgtgca gataggtatg
    23101 ctcttcagaa ccttccggga aggtgatgta cggcagtttt tcgatatctg catcagacac
    23161 cggcacattg aaacggtcgc ggatgtgacg cacgccgtcc atgttcattt tcttaacctg
    23221 gtgcgcgatg tttttacctt cagccgcgtc gcccatgccg taacctttaa tggtatgagc
    23281 aaggattact gtcgctttgc ctttggtttc ctgcgctttc ttgaacgcag cgtagatttt
    23341 cttcggatcg tgaccaccac ggttcagtgc ccagatctgc tcgtcagtcc agtctgcaac
    23401 cagtgctgcg gtttcaggat atttaccgaa gaagtgttca cgaacgtacg caccatcttt
    23461 cgatttgaag gtctggtagt cgccgtcaac ggtttcgttc atcagctgga tcagtttacc
    23521 gctggtatct ttacgcagca gttcatccca acggctaccc cacatcactt tgatcacgtt
    23581 ccagccagca ccttcgaaga tgccttccag ttcgttgatg atcttgccgt taccggtgac
    23641 cgggccgtca agacgctgca ggttacagtt gataacgaag accaggttat ccagtttttc
    23701 acgggtagcg atggtgatcg cacctttgga ttccggttcg tccatttcac cgtcgccgag
    23761 gaacgcgtaa acggtctgtt tagaggtatc tttcaggcca cggtgttcca gatatttcag
    23821 gaatttagcc tggtaaatag caccaatcgg ccccagaccc atagatacgg tcgggaactg
    23881 ccagaattcc ggcatcagtt tcgggtgcgg ataggaagag aggccattgc cgtgaacttc
    23941 ctgacggaag ttatccagct gctcctgagt cagacgacct tccaggaaag cacgagcgta
    24001 cacgcccggg gagatgtggc cctggaagta aaccaggtcg ccgccatcct gctcgttgcg
    24061 tgcacggaag aagtggttaa agcacacatc ataaatggtt gcggaagact ggaaggacgc
    24121 catatggccg cccagttcga ggtctttttt cgacgcacgc agcaccgtca tgatggcgtt
    24181 ccagcggata gctgaacgaa tacggcgttc cagttccaga ttacccggat actccggttg
    24241 ttcttcaacg gggatggtgt tgatgtagtt gctgatacct gtgcctgcgg ctacgtttac
    24301 accgcctttg cgggcttcag caagcagttg gtcgatcaga tactgagcac gctcaacacc
    24361 ttcttcacgg atgaccgatt cgatcgcctg gagccagtcg cgagtttcga tcggatccac
    24421 gtcatttggg aaacgttctg acatgggtta ttccttatct atctaataac gttgagtttt
    24481 ctggaacctg tcccattgaa ctctcgccgg aaagctcaat aagacaggtt ctacgtttag
    24541 ttgccgcgct tttatatgcg cttgatttac aacatcttct ggataatttt taccagaaaa
    24601 atcactaatt ctttcgttgc tccagacgac gcagagaacg ctcacggcgg ctctcttcac
    24661 gacttctgtc gagcaaaatt tcttcgataa aggccagatg gcgatgcgat gcttcgcgcg
    24721 cttcttccgg cttaccggcc ataatcgctt caaatatgcg ggtgcggtga ctactcacca
    24781 gcggcagcat ctcgcgacgc gaatagagca attcgaagtt ctggcggaca ttctgggcca
    24841 acatcggctc catacacctt agcagatgaa gcagaaccac attgtgggcc gcttcagtga
    24901 cagcaatctg atactggagt acggcgtttg attccgcgtc cagatcgcca gactgctgcg
    24961 ccagttctat ggcgtgatgg agttcacgga tgcgttcctt gtcttcatcg gtactacgca
    25021 gcgcggcgta ataagcggcg ataccttcca gggcgtgtcg tgtttcgagc aagtcatact
    25081 gtgactcagg atggtcggag agcagctcca ccagcggatc gctgaagctt tgccataggc
    25141 tgctctggac aaaagtgccg ccaccctggc gacgaagcaa caagcccttc gcttcgagac
    25201 gttgaatcgc ctcacgcaag gagggacggg agacgtcaaa ctgttttgcc agttcgcgtt
    25261 ccggtgggag tttttcgccc gggcggagag tgccttcgag gatcaaaaac tccagttgct
    25321 gctcaatcac atcggagagt tttggttggc ggattttgct gtaggccatg agttcctgtc
    25381 ttaagccact tgccgaagtc aattggtctt accaatttca tgtctgtgac gctaaagtaa
    25441 caaagtattc accttatgtc catacaggtt ttgattgaaa tcatgaaact gtgcacattt
    25501 taacaacttg acatatataa cgtttcaaaa ttgtaactat gcacaaatgt agactttacg
    25561 taggaaaggg attttttaac gattattaaa ctataaaaat ccaaattgaa ccgattcact
    25621 taccaatttt ttttgatttt taagtcaata aaaacgaatt taaattcatt ctgcatattg
    25681 agaggggttg aggctgagct ttacaaacgg tttcttttta agcaactcat cttcaaccat
    25741 gcataaagcg ggtgcattcg ctgccgcata ccattattct tgatctgacg gaagtctttt
    25801 tgtaacaatt caaacttctt tgatgtaaac aaattaatac aacaaacgga attgcaaact
    25861 tacacacgca tcactgcgta gatcaaaaaa acaatcaccg cacgaggttt catgatggaa
    25921 ggtcaacagc acggcgagca gctaaagcgc ggccttaaaa accgccatat tcagcttatc
    25981 gcgctgggtg gcgcgatagg gacagggcta ttcctgggta gcgcctccgt aatacagtcc
    26041 gcagggccag ggattatcct gggttacgcc attgctggtt ttatcgcctt tctgatcatg
    26101 cgtcagctgg gtgaaatggt ggtcgaagaa cctgtagcag gctcctttag ccactttgct
    26161 tataaatact ggggcagttt tgccggtttc gcctctggct ggaactactg ggtactgtac
    26221 gttttagttg ccatggctga gctgactgcc gtgggtaaat acattcagtt ctggtatccg
    26281 gaaatcccca cctgggtttc tgccgccgta ttctttgtgg tgattaacgc catcaacctg
    26341 accaacgtta aagtgtttgg cgagatggag ttctggtttg ccattatcaa agttatcgcg
    26401 gtggtagcga tgatcatctt cggcggctgg ctgctattca gtggcaacgg cggcccgcag
    26461 gcgaccgtta gcaacctgtg ggatcagggt ggtttcctgc cgcacggctt caccgggctg
    26521 gtgatgatga tggcgattat catgttctcg ttcggtggtc tggaactggt ggggatcacc
    26581 gcagcagaag ctgataaccc ggagcaaagt ataccgaaag caactaacca ggttatctac
    26641 cgcatcctga ttttctatat tggttcgtta gccgttctgc tctcactgat gccgtggacc
    26701 cgcgttaccg ccgataccag tccgtttgtg ctgatcttcc acgagttagg cgataccttt
    26761 gtggcgaatg cgctgaacat cgtggtactg actgcggcgc tctccgtgta caacagctgc
    26821 gtatattgca acagccgtat gctgtttggt ctggcacaac agggtaatgc gccaaaagcg
    26881 ctggcgtctg tcgataaacg tggtgtacca gtaaatacca ttctggtgtc tgcactggta
    26941 acggcgttgt gcgtactgat taactacctt gccccagagt ccgctttcgg actgttaatg
    27001 gcgctggtgg tatctgcact ggtaatcaac tgggcgatga ttagcctggc gcatatgaaa
    27061 ttccgtcgcg ccaagcagga acaaggcgtg gtaactcgct tccctgctct gctttatccg
    27121 ctgggtaact ggatctgcct gctgtttatg gcggcggtac tggtgattat gctgatgacc
    27181 ccaggaatgg cgatttcggt atacctgatc ccggtatggc tgatcgtgtt aggtatcggc
    27241 tatctgttta aagagaaaac cgccaaagcc gtaaaagcgc attaatctct ctacgccctc
    27301 acccgtacag ggtgagggca ataatcttta caccaacgcc ccgtaaatcg tcagtagtgc
    27361 aatcaccacc acgaccacga acgaggtttt cttcgccatt gaaaccgctg ccttcggcgt
    27421 ctccacctta tcgacatgcg gttcacgcgc cagagagaac tgcgccagac gcgttaacac
    27481 ctgatactgc gaagtatgga aatcacccag cgaagcaaac caggccggta acgctttctc
    27541 accatgaccg atcaaggcat ataccacacc cgcaagacga accggcaccc aatccagtac
    27601 atgaagcacg gcatcaatgc cggactgtaa acgatgatgc ggcgtctgat atcgcgccag
    27661 ccagtattgc catgcacgca aaaacgcata ccccatcagc gtaacgagtc cccaggttcc
    27721 ccccacaatc agccagaaca gcggtgcaag ataaaaacga aagttaatcc acagcaatgc
    27781 attttgcagc tcacgcaaat actcacgttc gtcgcagcct gccgggacgc cgtgaatcat
    27841 ggtgagttcg ccagccatcg tggcacgggc atggctatca ttacgtgaag cagctgtcag
    27901 ataagcatga taatgaagac gaactttacc tgcgccaata cacagcaaac caatcagcag
    27961 ccacaccagt agcgtgggaa cgttgaacaa tactccctgc aatgcgcgta acagtaaaaa
    28021 agtcacgccc atcgcaataa tggtcatgcc taacgtgcgc ccgagagaaa aatgtttcac
    28081 ccgccgaaag aacgcttcaa gacgatgatc aagctgccag tgctcgccca acttaaacag
    28141 gcgctcgaaa attaacacca gtaaggttgt aaatagcgtc atgttgtctc cttgctgacc
    28201 agcacacgaa accgtgccca atcaaatgca ggaccgggat cggttttccg atccggcgca
    28261 atatcacaat ggcccgtcat gtttttagcg atatccggat agcaatcaat cagtgcccgc
    28321 gtaaccgccg caagctgttg atactgcgca tcggtatacg ccagcgtatc ggtgccttca
    28381 agctcaatcc caatagaaaa atcattgcag cgttcgcgcc cctgatactg agagactccc
    28441 gcatgccatg cacgtttatc gaaaggaaca tactggacta tttcaccatc acggcgaatc
    28501 aaacagtgag cggagacgcg caaatgggcg atctcagcaa agaaaggatg tgcctgcgga
    28561 tcaatagttc cagtgaataa tgcgtcgatc cacggaccgc caaactcgcc tggcggcagg
    28621 ctaatattgt gcaccaccag cagggtgggt gtttcgtcat ccgggcggca atcgtaatgt
    28681 ggtgagggaa cgcggcgcgc gccaaccagc cacccctgtt ctaacaacat gcaggatctc
    28741 cttatatgtg gtgctaatac ccggttcaga gtagcatgtt tctaccttat gattcgttag
    28801 ctatctggag ttttaacatg ccgcctcgcc gctataaccc tgacacccga cgtgacgagc
    28861 tgctggaacg cattaatctc gatatccccg gcgcggtggc ccaggcgctg cgggaagatt
    28921 taggcggaac agtcgatgcc aacaatgata ttacggcaaa acttttaccg gaaaattctc
    28981 gctctcatgc cacggtgatc acccgcgaga atggcgtctt ttgcggcaaa cgctgggttg
    29041 aagaggtgtt tattcaactg gcaggcgacg atgtcaccat aatctggcat gtggatgacg
    29101 gcgatgtcat caatgccaat caatccttgt tcgaacttga aggcccatcc cgcgtgctgt
    29161 taacgggcga acgcactgcg cttaattttg tgcaaaccct ttcaggagtt gccagtaagg
    29221 tacgccacta tgtcgaattg ctggaaggca ccaacacgca gttgttggat acgcgcaaaa
    29281 ccttacccgg cctgcgttca gctctgaaat acgcggtact ttgcggcggc ggagcgaatc
    29341 accgtctggg gctttctgat gccttcctga tcaaagaaaa ccatattatt gcctccggct
    29401 cagtgcgcca ggcggtcgaa aaagcgtcct ggctgcaccc ggatgcgcca gtagaagtcg
    29461 aagtagagaa tctggaagaa cttgatgaag ccctgaaagc aggagccgat atcatcatgc
    29521 tagataactt cgaaacagaa cagatgcgcg aagccgtcaa acgcaccaac ggcaaggcgc
    29581 tactggaagt gtctggcaac gtcactgaca aaacactgcg tgaatttgcc gaaacgggcg
    29641 tggactttat ctccgtcggt gcgctaacta aacacgtaca agcactcgac ctttcaatgc
    29701 gttttcgcta atagttgaca gatttatccg ctccatcgcg ggcggataat ctccccttcc
    29761 ccaactttct tcgtaacgcc tcgcaaattt cttgtctttc gctgcaacga aataaaaaac
    29821 ctcggaatct gctttcccgt ttcgtttttt gcccctcgcc tgtgctgatg gcgtttgcca
    29881 aagtagcgcc aaccaaatca aggagcgaaa cagatggaca agcaacgcgg ttttacactt
    29941 atcgaactga tggtggttat tggcatcatt gccattttaa gcgccattgg tattcccgct
    30001 tatcaaaact acctgcgcaa agccgcactc accgacatgc tacaaacctt tgtgccttac
    30061 cgtaccgccg tagagttgtg cgcgctggaa catggtggat tagatacctg cgacggtggc
    30121 agcaatggca ttccctcgcc taccaccacc cgctatgttt cagccatgag tgtggcaaag
    30181 ggcgtggtgt cgctgaccgg gcaagaaagt ctcaatgggc taagcgtcgt catgacaccg
    30241 ggttgggata acgcaaacgg tgtcaccggc tggacgcgca actgcaatat tcaaagtgac
    30301 agcgcattgc agcaagcctg cgaagatgtc ttccgctttg atgacgccaa ctaaggagcg
    30361 gcaatgaata ttccacagct cactgccctg tgtctgcgtt atcatggagt cttgctgaat
    30421 gccagcgaag aggtggttca tgttgcggta gtcgatgcac cttcgcatga gctactggac
    30481 gcattgcatt tcgctaccac caaacgtatt gagatcacct gctggacgcg ccaacaaatg
    30541 gaaggtcacg ccagtcgcac acaacagaca ttgcccgtag ctgttcagga gaagcatcag
    30601 cccaaagcag agttgctgac tcgaacgtta caatctgcgc tggaacaacg tgcgtctgat
    30661 attcatatcg aaccagcgga caatgcctac cgcatccgct tgcgtatcga cggcgtattg
    30721 catcctttac cggatgtttc accggatgcc ggagtcgcat taaccgccag attaaaagtg
    30781 ctgggaaacc tggatattgc ggaacatcgc ctgccgcagg acgggcaatt cactgtcgaa
    30841 ctggcaggaa acgccgtctc atttcgtatt gcgaccttac tatgtcgggg tggtgaaaag
    30901 gtggtattaa ggttgttaca gcaggtgagc caggcactgg atgttaacac gctgggaatg
    30961 cagccgttac aactggcgga ctttgctcat gccttgcaac aaccacaggg actggtgctg
    31021 gtaactggcc ctaccggcag cggcaaaacg gtcacgcttt atagtgccct gcaaacgctg
    31081 aataccgctg acattaatat ttgtagcgtc gaagatccgg ttgagatccc catagccgga
    31141 ctaaaccaga cgcaaatcca tccgcgtgcc ggactcacct ttcagggcgt tttgcgtgcg
    31201 ttattgcgcc aggatcctga cgtcatcatg atcggagaga tccgcgatgg cgaaacggca
    31261 gaaattgcca ttaaagccgc gcaaaccggt cacctggtgt tgtctaccct acacactaat
    31321 tccacctgcg aaacgctggt acgtttacag caaatgggcg ttgctcgctg gatgctctca
    31381 tcagcgctta cgctggtaat agcccagcgt ctggtacgta aactttgccc acattgtcgc
    31441 cagcagcaag gggagcccat ccatattcca gacactgtat ggccgtcgcc gctgccccac
    31501 tggcaagcac ccggttgtgt acattgctac cacggttttt atggtcgcac ggccttattt
    31561 gaagttctgc ccataacgcc ggtcatacgt cagcttattt ccgctaatac cgacgttgaa
    31621 tcgctggaaa cgcacgcacg acaggcgggt atgcgtacgc tttttgaaaa cggctgcctg
    31681 gccgtggagc aaggcttaac cacctttgaa gagttaatcc gcgtactggg gatgccgcat
    31741 ggcgagtaag caactctggc gctggcatgg cataaccggc gacggcaatg cgcaagatgg
    31801 gatgctatgg gcagagagcc gtgctttgct gctcatggca ctacagcaac agatggttac
    31861 cccactaagc ctgaagcgaa tcgccatcaa ttctgcgcag tggcgaggag ataaaagcgc
    31921 ggaagtcatt catcaactgg cgacgctact caaagcaggg ttaacgcttt ctgaagggct
    31981 ggctctgctg gcggaacagc atcccagtaa gcaatggcaa gcgttgctgc aatcgctggc
    32041 gcacgatctc gaacagggca ttgctttttc caatgcctta ttaccctggt cagaggtatt
    32101 tccgccgctc tatcaggcga tgatccgcac gggtgaactg accggtaagc tggatgaatg
    32161 ctgctttgaa ctggcgcgtc agcaaaaagc ccagcgtcag ttgaccgaca aagtgaaatc
    32221 agcgttacgt tatcccatca tcattttagc gatggcaatc atggtggttg tggcaatgct
    32281 gcattttgtt ctgccggagt ttgccgctat ctataagacc ttcaacaccc cactaccggc
    32341 actaacgcag gggatcatga cgctggcaga ctttagtggc gaatggagct ggctgctggt
    32401 gttgttcggc tttctgctgg cgatagccaa taagttgctg atgcgccgac cgacctggct
    32461 tatagtgcgg cagaaattgc tgttacgcat cccgattatg ggttcactga tgcggggaca
    32521 aaaactcacg cagatcttta cgattctggc gctgacacaa agtgcaggca ttactttttt
    32581 gcagggcgta gagagcgtca gagaaacaat gcgctgcccg tactgggtgc aacttctgac
    32641 acaaatccag cacgatatca gtaacggtca tcccatctgg ctggcgctaa aaaataccgg
    32701 ggagtttagc ccgctctgtt tgcaattagt gagaacagga gaggcatccg gctcgctgga
    32761 cctcatgtta gacaacctcg cccatcatca tcgggataac acaatggcgc tggcggataa
    32821 cctcgcagcc ttactggaac cggcgttgct gatcataacg ggaggaatta tcggtacgct
    32881 ggtggtggcg atgtatctgc caattttcca tttaggcgat gcgatgagtg ggatgggata
    32941 acgctggcgt gttgctccac accagcgttg gaagattaca ggttgttgaa gatgcggttt
    33001 tcttgttcct gcacacggat aaatgtggtg cgcttggtca gttctttcag gcgtgaagcc
    33061 ccaacgtatg tacaagctga acgcaggccg cccaaaatat cacgcgcggt attttcaacc
    33121 gggcctcgca gcggcagctt aacggtttta ccttctgctg cgcgatattc cgcaacgccg
    33181 ccaacgtgac gtttcatcgc agactcggag ctcatgccgt agaacagcat aaatttctcg
    33241 ccgttctcct caacgatgcg accgccgctc tcttcgtggc ccgccagcat gccgccaagc
    33301 atgacgaaat cggcaccgcc gccaaaggct tttgcgacat cgcccggcgt ggtgcagcca
    33361 ccgtcgctga cgatcattcc gcccagaccg tgcgccgcat cggcacattc gattaccgca
    33421 gaaagctgcg gataaccgac gcctgttttg acgcgagttg tacaaacaga acctgggcca
    33481 atgccaactt taacgatatc agcaccggag aggataagct cctcacacat ttcaccagtc
    33541 actacgttgc cagcacaaat ggttttggtc ggccacgctt cacgcgcttt cgcaacgaac
    33601 tgcacgaagt gttcggaata accattcgcc acatcaatac aaacgaagtt taatgccggg
    33661 ttcaggtcga gaatctgttt agttttttcg aaatccgcat cagacgtacc ggtagaaacc
    33721 atcacatgtt tcagcacatc agcggaagaa ttgttgataa acgcttgcca ctcttcgaca
    33781 gaatagtgtt tatgcacagc agtcaaaata tcaaaagaag ccagcgcaga ggccatagaa
    33841 aatgtaccta cggtgtccat atttgcggcg ataatcggca cgccggacca gctctgacct
    33901 gaatgtttga aggtgaattg acgttccagt tcaacatcgg aacggctttt aagagtggag
    33961 cgtttagggc ggatgagaac gtctttaaaa cctaacttca gatcttcttc aatacgcatg
    34021 tgcggattcc tggggttaat ggctataagg gtaagagcgc aactccagtg acgctatcat
    34081 acgcactaat caatgctgcg caagactgcg aaattgtctt tttttacgct acaatcccat
    34141 aaatttacct ggtaaatagt gagcgaacgt ttcatcaatt tcgctttcgt gcactcattt
    34201 ttaacgtttt gataatcatg atgaattccc gggaatataa attatgaggt atatagttgc
    34261 cttaacggga ggcattggca gtggcaagag taccgttgcc aatgcgtttg ctgatctcgg
    34321 aattaacgtc attgatgccg atattattgc gcgtcaggtg gttgaaccag gtgcacctgc
    34381 gctacatgcc attgctgatc actttggcgc taacatgatt gctgctgatg gaacattgca
    34441 gcgccgggcc ttgcgcgagc ggatcttcgc caacccggaa gagaaaaact ggcttaacgc
    34501 cctgctgcat ccgctgattc agcaagagac gcaacaccag atccagcaag ctacttcccc
    34561 ctatgtactg tgggttgtgc cattgctggt agaaaactca ctgtataaaa aagcgaatcg
    34621 agtgctggtg gtggatgtca gcccagaaac gcaacttaag cgcaccatgc agcgcgatga
    34681 tgtaactcgc gagcatgtcg aacaaatcct tgctgctcag gcaacgcgcg aagcccgcct
    34741 tgccgtggca gatgacgtca ttgataataa cggcgcaccg gatgctatcg catcggatgt
    34801 tgcccgcctg cacgcacact atttgcagct tgcgtcgcag tttgtctcac aggaaaaacc
    34861 gtaatgcaga cccaggtcct ttttgaacat ccactaaatg aaaaaatgcg tacatggctg
    34921 cgcattgagt ttttgattca gcaactcacc gttaatttac ccatcgttga ccacgctggc
    34981 gcgctgcatt tcttccgtaa tgtcagtgaa ttactggatg ttttcgagcg cggcgaagtc
    35041 cgcactgagc tgttgaaaga acttgaccgg cagcaacgta aactccagac ctggattggc
    35101 gtgcctggcg tggaccagag ccgtattgaa gcattaattc agcagttaaa agcggcgggg
    35161 agcgtattaa tttccgcgcc gcgtatcggg caatttctgc gtgaagatcg tttgattgct
    35221 ctggtgcgtc agcgactgag catcccaggc ggctgttgca gctttgattt acctacattg
    35281 cacatttggc tgcatctacc ccaggcgcag cgcgacagcc aggtagaaac ctggattgcc
    35341 agcctgaacc cgctcaccca ggcacttacc atggtgctgg atttaattcg ccagtcggcc
    35401 cccttccgta aacaaaccag cctgaatggt ttttatcagg ataacggtgg cgatgccgac
    35461 ttgctgcgcc tgaatctgtc gctcgattca cagctttatc cgcaaatttc cggacataag
    35521 agccgttttg ccattcgttt tatgccgctg gacagtgaaa acggacaggt accggaacgt
    35581 ctggatttcg aactggcctg ttgctaagga gtgaaaatgt cagaaactat tacggtgaat
    35641 tgcccaacct gcgggaaaac ggtggtgtgg ggtgaaatca gcccgtttcg gccattttgc
    35701 tccaaacgtt gtcagctgat cgacctcgga gaatgggctg ctgaagaaaa acgaatcccc
    35761 agcagtggcg atctttccga aagcgatgac tggagcgaag agccaaagca gtgacattgg
    35821 tctgatgccg cacgtaggcc tgataagacg cggacagcgt cgcatcaggc atcttgtgca
    35881 aatgtcggat gcggcgtgaa cgccttatcc gacctacaga ctacagacgc ttaagcttcg
    35941 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccaacc agcgacatcc
    36001 actcaccggg ttgcccttct ttaccccacg gcaccccttc ccaactttcg accagccaga
    36061 accacagagt tatatgcctg tccgggaatt catatgccag tttttcaaat agcgaaaaat
    36121 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    36181 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    36241 cgcgacgcgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    36301 gctttttcat tgttttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttgcttcaac
    36361 agttagcttt tattgcaggc ggccatgaca ctgcttgtat tttttaccag aaccgcacgg
    36421 gcaaggatcg ttacgtccta ctttgcgctc accggtttgc gccgccagtg cagctgcggc
    36481 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgc gctaaacgct cggcttccat
    36541 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    36601 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    36661 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    36721 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    36781 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    36841 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    36901 atccagccac tcggcaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    36961 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    37021 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    37081 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    37141 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    37201 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    37261 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    37321 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    37381 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    37441 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    37501 cgcggcaact tctgcctgcc agctaccacc gagcacaata tctgtaccac gacccgccat
    37561 attggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    37621 ggcgtggaat ttggcgttca ggacgttgtg cttaataccg gctttggtca gttcgtttga
    37681 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    37741 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    37801 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagctt
    37861 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    37921 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    37981 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgacggcc
    38041 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    38101 gtcgacgtcg cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    38161 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    38221 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    38281 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    38341 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    38401 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    38461 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatattgt cgcgcaggta
    38521 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    38581 tgccggtgct ggcatgcccg gcaggttgat accgacagtc aggccaagga attcaaacag
    38641 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    38701 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    38761 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    38821 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgtaccacgg cgaaagcttc
    38881 cgggatcaga ttttccagca cttcgccttt ttccagacgt gcacgaaact ctgcggtttt
    38941 ccctttcagt tcttcgtcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    39001 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    39061 caatttgatt agcataataa aatctcaaac gccccgcgtt gcggagttaa taaaatgaag
    39121 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    39181 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    39241 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    39301 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggctgtgtt tgcggtgcca
    39361 ttgcgaaaga aagatgacgg attaccgtgc gaatggcatg ttgatgccag taatcaacgg
    39421 aatagttcga attcgggcgg cgtgtgttcg cttccagcaa ggccaattga ccaaagttaa
    39481 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcacgttt ggttcggcgg
    39541 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    39601 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    39661 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    39721 gcatgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    39781 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    39841 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    39901 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    39961 ctgtttcgcc aagacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    40021 gataaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    40081 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    40141 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    40201 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    40261 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    40321 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    40381 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    40441 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    40501 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    40561 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    40621 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    40681 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agagtgactt tcttgccggt
    40741 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    40801 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    40861 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgcagga
    40921 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacga
    40981 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    41041 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    41101 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    41161 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    41221 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    41281 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    41341 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atctcagaca
    41401 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcag
    41461 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    41521 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    41581 catgcttgga cagttcagtg atcccctgct ccgcgaatgc catacgcttc ttgccttcaa
    41641 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    41701 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    41761 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    41821 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    41881 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    41941 caccttcaat gcgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    42001 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    42061 gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc tcataaaaat taaaactctt
    42121 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    42181 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    42241 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    42301 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    42361 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    42421 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag cagctcggta tagcgcggct
    42481 cgatcacctc tgccagtgtc tgacgttgca gactccgtgg cggacgacca cctacgctcg
    42541 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    42601 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    42661 cattgccagc ataaggaatt accttagtgt ggcgcaatgc cccaccggta taaacggcga
    42721 tatccattgt accaccaccg atatcgacga cgcagacccc cagttcacgt tcatcttccg
    42781 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    42841 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    42901 cttttgcctg catccgcacg cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    42961 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    43021 atttcgcggt atggacgacg ttttccacat cttcttgcgt tacttcttct tcagaaatag
    43081 gcaccatacc aatttcattc tggcagctga tatgcttacc agaaagcgcc agatataccg
    43141 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgctgt acgcacttga
    43201 ccacggattc gaggtcgttc accccgcctt tatccatacc acgcgacggg cagctgccca
    43261 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    43321 cggtaccaat ctccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    43381 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    43441 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    43501 ctgctgctgt aaaaccggat aaagttctac aaagcgagcc aaacgtttca tcgtatcgcc
    43561 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    43621 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    43681 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    43741 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    43801 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    43861 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    43921 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    43981 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    44041 ttgcgcatct tccatccagc ccaacacgac ccagccgctc accaacactg tcgttaaaac
    44101 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    44161 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    44221 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    44281 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcgctgt
    44341 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    44401 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    44461 tctcatcaga gagatacttc gcctcataat catagaaggt tctggccggt tgaatacgta
    44521 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    44581 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    44641 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    44701 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    44761 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagcagtt
    44821 tgctgcgtag tttatccatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    44881 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    44941 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    45001 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    45061 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    45121 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    45181 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    45241 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    45301 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    45361 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    45421 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    45481 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    45541 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    45601 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    45661 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    45721 cctggcgcat tcagggtgac gcgcatcggc tctttgtcct ggcgcagcag cgtaaagtgc
    45781 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    45841 taagtcgtgg tctgacgtcc cactcgcggt aacaattcgc ggatcaccgg atcatcaaca
    45901 cacatcaccg cacgaccgta aaacggcagg ttgtgcagaa aattaataaa agtctgtttt
    45961 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    46021 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    46081 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    46141 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt ggttgtcgtt
    46201 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    46261 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttatcg
    46321 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    46381 ttgaaataaa tcgtcgctcc cagattcatt aactgctgcg tgaccggatt tggcgctaaa
    46441 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    46501 gcaccaccga tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    46561 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    46621 gatgcaaaag gcatcgctac aattacgccc gggcagcgcg gctcacttca tttgccactc
    46681 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    46741 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    46801 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    46861 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcaccgtta
    46921 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    46981 taaattccgt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    47041 gttgcgaacc tttaccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    47101 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accagcacac
    47161 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    47221 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    47281 ctttggtggc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    47341 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    47401 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    47461 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcagac
    47521 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    47581 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatgg tgcgcaaccg
    47641 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttccct
    47701 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    47761 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    47821 gatcagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    47881 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    47941 gtcaatttct aacgctttgc ggccaatcga catcgcgcga aaagcgacga agaataccat
    48001 taaaagtgcc agcaccacac cgacataccc cagttcttcg ccgataatgg cgaaaataaa
    48061 gtcagtgtgc gcttccggca gatactccag tttttgtacc gagttaccta aaccttgtcc
    48121 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    48181 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    48241 tatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    48301 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacactacca ccgtaccaag
    48361 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    48421 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    48481 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    48541 acgcgatgcc cctttaaccg agctacccac taccagcacg atcatcagca ggatgataga
    48601 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    48661 gatcgccaga ataaacgcca aaatcagata gacgccatca cgcttcgcga agaagaacgg
    48721 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    48781 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    48841 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    48901 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    48961 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    49021 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    49081 cgcatcgcct gttccatagt ttcggtttgt tctgccactt ccaggcgtag cgccgccaac
    49141 tgcgcaccgt cacgaccaaa acagtacaaa cgcacgttgt cgccattcag atagcgcgcc
    49201 agtggactga agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    49261 acctgcaagc cattcaacgc cgcttccgta ctgccgacgt tagtggcttt cgaatcgtta
    49321 atccagcgta cgccattgtg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    49381 gtggttaacg ctttcaggct gctggcgcgc ggcaaaccag ctgcgtctgc cagtgccagt
    49441 gctgccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacgttcagc
    49501 actttctcgc ctttcactcg cagccaggtt tctccctgct gatgattcag gtgatagtca
    49561 cccatgttga cgccaaagct gacgcagcgt tcatcagcac cgcgaatcgg cattgttaag
    49621 gcatcatcag cattgacgac acaaactttc gcattttcgt aaatacgcaa ttttgccgca
    49681 cggtattgct gcaaaccaaa cggatagcgg tccatatgat cttcagtcac gttcagaatg
    49741 gtcgccgcca ccgcctgtaa gctggaagtg gtttccagct ggaagctcga cagttccagc
    49801 acgtacagtt cgcactcagc atcaagcagc atcaacgctg gcaggccaat attgccaccc
    49861 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    49921 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca gaacagctcg
    49981 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    50041 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    50101 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    50161 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    50221 aggcccaggc cgataatgac gacattttta ccctgataat cagccatgat taacgtacct
    50281 tcagcgttgc cagaccaatc agaaccagca tcagcgaaat aatccagaaa cgcacaatga
    50341 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    50401 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    50461 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    50521 ctaacgcacc acctaacgcc agcgaaccta catcgcccat aaagacctgc gccggatagg
    50581 tgttaaacca caggaagccc agtcctgccc cgactatcgc cgtgcagaca atgaccagtt
    50641 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    50701 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccaggccgt
    50761 cgagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    50821 gaatgtagaa cagccccagc tgcggcatca catctttaaa gaatgggacc accagctgcg
    50881 ttgcgggcgt gtctttgccg gcaaggtaca gggcgaaggc gacacccagc gcaatgaccg
    50941 acatccagaa atacttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    51001 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    51061 cgtacggatt ggacgggtaa gcccacagca gtacggagat cacaatcgcc gtcaggatca
    51121 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    51181 gcaccacctg accaaaggaa agtttttgca aatgagcaat catacgcggg cccatccaca
    51241 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    51301 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    51361 ctcctgtaaa gcgcgtacta cctcttccat ggcggcacta cgtgaaccct taactaaaat
    51421 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    51481 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccacgct
    51541 taacacgcgg tcaataccag cagcttttgc cgcctcgccc acctgtacat ggcaggcttc
    51601 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    51661 agccagtacc tgaacagcgg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    51721 cagcaactgg ttttctgcca gttggatggg gaacagacgg cctggaacag ctttcagatt
    51781 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    51841 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    51901 ggttggggtt tgtagggtaa attccgtacc gtgcgaggtc acatggatat tggtggcggt
    51961 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    52021 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    52081 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    52141 atgcgccgct gccaggttgt tgaccagcgc agcttccggg cgagtcagac tcacagtcca
    52201 ggctatttcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    52261 taagcgcaac aacgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    52321 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    52381 ggaacctgtc agagcaacca cgcgcgccgg aacttgctgg cgaacccatg cagccagttc
    52441 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatat ccagcggacg
    52501 gctgaccagt agtgcgcccg cgccaccagc tttcgcctgg tcggcaaaat catgagcatc
    52561 gaaacgttcg cctttcaggg caacaaacag gcagcccggc gtcagttttc cggtatcagt
    52621 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tgtcggtaag
    52681 ttggctaagg gttacgctaa tcatgcaatc acccccagca gacgcgccac cgtgacgcga
    52741 tcggagtagt ccagacgctg attgccaaca atctggtaat cttcatggcc tttgcccgcg
    52801 accagcacca catcgttctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    52861 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    52921 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    52981 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    53041 acgcaccaca gcttgcccgc acagtgcagc cgcgccgcct gtaaggcttt ttccagtgca
    53101 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctagtgcagt aaacacttcc
    53161 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc caacggatag
    53221 cccagcgcca acagcgtcgc cagcgcgagc agcaggttgc tgacgttaaa agcgcccatc
    53281 agatggctct caatttcgcc atcgccccaa cttgagctaa agcgaatcgt cgcaccgctg
    53341 tcgtgatagt tcacgtcgtt cgcttttaac cagcgtccgt gacagttcgg attgatatga
    53401 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    53461 tcgtcggcgt taataatcgc ctgaccgcaa tgatgctcag aataaagcag ccatttcgcg
    53521 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    53581 accgacgccg caaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    53641 tccattgcgc aaaacgtcgc gccctgatcc accagccccg ccagctcgtg ctgaacatcg
    53701 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    53761 gtacccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag cagctgggta
    53821 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    53881 tggtaaaagc ggcccgccag tgcagataaa cgctcattga gctggctgag atagatgacc
    53941 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    54001 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    54061 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    54121 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    54181 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    54241 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    54301 cgctgaaact gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    54361 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    54421 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    54481 tttaatcgcc gccttcacgc cgccgccgcc cggtagcgcc acgctttcca tcatatgcac
    54541 cacggtgcgg acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    54601 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    54661 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    54721 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    54781 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgctaacgc
    54841 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    54901 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    54961 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    55021 tgagccaggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    55081 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt caccggtgtt
    55141 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cattcttcag
    55201 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgcagc
    55261 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    55321 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    55381 ttgactatcg acgttagtaa agccaatgag gtgagcagtc acttcgccgg atggataata
    55441 acggcgagat tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    55501 catgtcaggg tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    55561 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    55621 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    55681 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    55741 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    55801 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    55861 taacaacgca aaacgccaac taataaagtt ggcatgttct tcctgacgtt ttggtttctg
    55921 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    55981 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    56041 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    56101 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    56161 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    56221 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    56281 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgcg
    56341 atacgcagaa ctgaactacg ggcacgagga ttctcagcca cctcttcttc gcccggcatt
    56401 aacttgccta atgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    56461 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    56521 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    56581 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    56641 gcctggaagg tacgagtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    56701 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    56761 gcgcgtgcaa tgcgtttggc aaaacgctct tcaccatagg ttttcaatac ccaggcgata
    56821 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    56881 cgcatgtcca gcggaccatc gcgcataaag gaaaagccac gttcagcatc atcaagttgc
    56941 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccgataag atcgcgctcg
    57001 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    57061 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    57121 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    57181 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    57241 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    57301 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    57361 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    57421 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    57481 atgacctaac agtaggcgct gcacacggcg ctcaactggg ttcatgctcg acagacgcga
    57541 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    57601 aatgtcaatg gtgcaaacca tttgaccggc agtcccgata acgggtaggc actgataagc
    57661 gccctttgct gtcgagattg actaacgttg ctccccggaa catgccagcc tcacccctta
    57721 ttcccacttt gccccacaat ttcccactga aaggagttta cggagcggag gaaaagcttg
    57781 tcaagccagt cacaaggcat accgaggtaa aaaacccgat gtttacggct aatatcagcg
    57841 ttgtttattt tacgtccaac gtgcgaagca aattaacgtt atgaatagct gtaagaaaaa
    57901 atccctcatc gtcatcactg ttttaaaatg actcactatt ttccacagca aatataaagt
    57961 gtcagtttgc gacgcgagcg gcattttagg acatatcttc cccggttaac agtccttgtt
    58021 acgtctgtgt ggcgcttgat gccagcgggt ttgcgcaaaa gcaaagaaaa gtgtttgtta
    58081 aatcggcaat tcatcgccgc atgtaacaaa ataatgcaaa aacgcgctgt cgatatcact
    58141 taattattgt gaatttcagc tggtgagtta attaacgggc gggtttaaaa ccatttatca
    58201 ataatagtca ggaatagtct tatttacttt aagcatattg atgtccagtc ccgtactcta
    58261 cgcgccagag ggaaattcac ctggcgcgta tttttgttcg cggcttagct acggctgagc
    58321 acaccgcggc gatagagatt acgtttaatg cgcgttaaac caggttttgg cttacgcggt
    58381 tcgtccaagc ttgccaggac aatctccagc acacgctctg cgacatcgcg gtgacgttga
    58441 gccactgcca gcaccgggca ctgtaagaag tcgagcagtt cgttatcgcc aaaggtggca
    58501 attgccaggt cagaaggcag tttaccatcg cgacgtagcg tgacatccat caccccttgc
    58561 aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg gatgcgtttc cagccatttt
    58621 tcgaataact gggcagccgc ctcccgctca tagctgttgg catacaggaa atgcacttcg
    58681 cgcggatcat ctttccaggc agtacggaaa ccttgttcac gcaggaagct gacagaaagc
    58741 tccggcagcg cgccaagata aagcaccgtc tcggcgggaa acttacgtaa ctcttccgcc
    58801 agcatttcgg catcatcctg atcggcacca accacgctgg tgaaatgttc acgatcgagg
    58861 gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc agcgttgata aaaaggatgc
    58921 tcaggtggca acgacgtcga aacaataatg gcatcaacct gacgctgtaa aaggtgctca
    58981 atgcagcgca tttcgttgtc tggctgatct tctgagcagg caatcagcag ttgataaccc
    59041 cgttgccgcg cctggcgctc aagatagtta gcgatgcggg tatagctggt gttctccaga
    59101 tcggggatca caagaccaat agaacgtgtg cgtccagcac gaagcccagc tgccacggcg
    59161 ttcgggtggt aattgtgctc acgcaccaca gccatgactt tttcaacggt tttgtcgctc
    59221 acacggtatt gcttcgcttt gccgttaata acatagcttg cagtggtccg cgacactccc
    59281 gccagccgag cgatttcatc cagtttcaca attgcccctt gcgtaaaatg taaaaaccat
    59341 agccattgta ctggtatggg ttaaattcat taacatctaa gcgcagaaaa ttaactgcgg
    59401 caactgcttt tattccaagt tcccactgat ttcgcaaaaa aagcccaacg tgacatgttg
    59461 ggctgcaaat tgcgcactga gatcattctc aacgcattat tttatcgccg cgcgaaagtc
    59521 cgaccacacc agagcgagca acttccacaa ttttcgccac atcgcgaatc gatgctaaaa
    59581 atgcatcaag cttatcgctg gtgcctgcta attgaacggt ataaagcgag ggggtgacat
    59641 cgataatttg cccacggaat atttccgtat tacgtttcac ttcgtcacgc ccgtaaccgc
    59701 tggcctgaat tttcaccagc atgatttccc gctcaacatg cgcgccctgc cccaactcac
    59761 tcacgcgcaa gacatcgacc agcttgtgta attgcttttc gatctgctca agtacttttt
    59821 catcgcccac ggtctggatg gtcatacgcg ataatgtcgg atcgtcggtt ggcgcaacgg
    59881 tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc aatcacgcgg gataacgcgc
    59941 ctgattcatt ttcgagtaag actgataata tccggcgcat aatcaggttc tctccgtttt
    60001 gcttaaccac atttcatcca ttccgccccc gcgaatctgc atcgggtaga cgtgctcact
    60061 gccatcgacg gtaacatcaa caaacaccag gcgattattg cgcacctgtt ccagcgcctc
    60121 gctaagtttg ctttccagct catgcggatg agagatctgg atcccgacgt gaccataggc
    60181 ttccgccaga cggacgaaat cgggtagtga ttgcatataa gattgtgaat gacggccgga
    60241 atagatcatg tcctgccact gtttcaccat ccccagatag cggttattga gattcaccac
    60301 cagtacaggc aactcgtatt gcaacgcggt agacagttcc tgaatgttca tctgaatact
    60361 gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac gccattttga cgcccagtgc
    60421 cgcaggtaaa ccaaaaccca tcgtgccgag gccaccggaa ttgatccagc gacgcggttt
    60481 gtcgaatgga taataaagtg cagcaaacat ctggtgctgc ccgacatcgg acgtcacgta
    60541 agcgtctccc ttcgtcaacc gccaaagagt ctcgatcacc gcctgcggtt taatcttttc
    60601 actgtgagtg tcatatttca ggcactgacg agcgcgccac tgttcaattt gctgccacca
    60661 gtcgcggatc tcatccagtg gttgatgggc ggattcttgc gacaagagtt caagcatttg
    60721 ttcgaggacc tggcgagcat cccccacaat cgggatatcc gcagtcacgg ttttagaaat
    60781 ggaagtagga tcaatatcga tatgcagaac agtggcattt gggcagtact ttgccagatt
    60841 gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa atcacatccg cgttatgcat
    60901 cgtcatattg gcttcgtagg taccgtgcat tcccagcatg cccagtgcct gacgatgcgt
    60961 tgccggaaac gcccccagcc ccatcaatga ggaaacaacg ggcagattca acgcctccac
    61021 cgtttctttc aactgctgat ggcagcccgc cgtgattgcc ccaccgccta cgtagacaac
    61081 cggttttttt gccgctacca gcgtttgcag agcgcgctta atttgccctt tatgtccggt
    61141 agtagtggga ttgtaagaac gcatactgac cgactccggc cagacatagg gtaatttgtt
    61201 cgccggatta agaatatctt tcggtaaatc aacgactact ggtccaggac gaccacttgc
    61261 cgccagccag aaagcctttt tcagcacctg cggaatgtct tccgtttgct taaccagaaa
    61321 actgtgttta accactggtc gcgaaatccc caccatgtcg cactcctgaa aggcatcgta
    61381 acctatcaac gaggtcacta cctgcccgga aaggacaact aatggaatgg aatccatata
    61441 agcggtggcg atgccggtaa tcgcattggt cgcccctgga cccgacgtta ccagcacgac
    61501 gccgacttcc ccggtcgcgc gcgccagacc atcggccata tgcactgccg cctgctcatg
    61561 acgaactaaa acatgatcga taccacccac ggtatgcaat gcatcgtaaa tatcaaggac
    61621 tgcgcctccg ggataaccga atacttgttt aacgccctga tcgataagcg atcggacgac
    61681 catctcggct ccagacaaca tctccatggc ctgcctcact gtttgatgga aaaaatgtgt
    61741 aaaacacatt aaccgtccga tcttgcttaa gcaattggca gccagagaca taaacgcagg
    61801 gtaataagaa taaacaggag gaaaggtgaa aagaaacgat aaaatcctcc attacgaagg
    61861 attaaattga taataaatcg gctgaatccc acaacttaca gcaatttctc tattttttta
    61921 attaatccta aaatagagag cccacacatt cagaataaaa ttctacattc aataaaaaat
    61981 gcagaataaa ccagacattc atgtctgacc tattctgcat tcagttagcg tttgcaaatt
    62041 gagactaatt gttcttccat ccactgatgg cctttatcgc gcccggcagc ttcatgccag
    62101 gagagataac aggttctgct gttttgtttt aacggcagcg gtaatacctg taattctaag
    62161 gattcagcga actcttcagc cagccaacgc ggcgcaatag cgaccaaatg cgtttgcgac
    62221 accacgctaa gtacgctcat cattgccatg ccctgatacg cgatactggc ttgcttatct
    62281 accgtgtcat accaaggttg actaaatgac gcgaaacgat cgagcgaaac cgccgcatgt
    62341 tgttcgttat aaacatcatg tttcagtaac gggcctttaa ttgttggatg atttttgctg
    62401 gctaccagca ccatttcatc tttaaataat ggcacgctgg taaattcagg acgatggaag
    62461 tcttcataac taatcacaaa ctccgtttcc tgataacgca gctgatgttc agtgttctga
    62521 tttaatgaag acttgaacat aacatgtata tttggcgcaa tctgctcaat gtgattataa
    62581 atctgcgagg tcagaatgct gtctaacggg ctgcaaacac aaagatgaaa tacacgttca
    62641 ctactcgcgg gttcaaaacc tgaaccaggc aattcatttt gtactagttg caatgcctgg
    62701 cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt gaataccacg gccataacga
    62761 acaaaaagct cgtcattaaa catcacctca ggcgtgcaac agcgttactg accgcaggtt
    62821 gcgacatacc cagaacatga gcggcacggg taatgttttg ctcctgcatc acggcatcga
    62881 aaacggttaa taagttgaga tcgaccatgc gtagctgtgg cttgcttaac tccgccgtct
    62941 ctgaatgatc tgtttgtacc tctggcatac ttaactccac tgtcacactt aactcccttt
    63001 cccttattgg aatgcagaga aataa
//
LOCUS       DAZKHC020000016        59286 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZKHC020000016 DAZKHC020000000
VERSION     DAZKHC020000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 59286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.016"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..787
                     /locus_tag="ACHOSM_RS06040"
                     /old_locus_tag="pgaptmp_001208"
     CDS             <1..787
                     /locus_tag="ACHOSM_RS06040"
                     /old_locus_tag="pgaptmp_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HGU4557776.1"
                     /translation="YYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELA
                     ENGIIVGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLNQTFAPAAPNQVW
                     VADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGL
                     IHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFN
                     NRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            833..2029
                     /locus_tag="ACHOSM_RS06045"
                     /old_locus_tag="pgaptmp_001209"
                     /pseudo
     CDS             833..2029
                     /locus_tag="ACHOSM_RS06045"
                     /old_locus_tag="pgaptmp_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            2026..2940
                     /locus_tag="ACHOSM_RS06050"
                     /old_locus_tag="pgaptmp_001210"
     CDS             2026..2940
                     /locus_tag="ACHOSM_RS06050"
                     /old_locus_tag="pgaptmp_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU4557777.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            2972..3220
                     /locus_tag="ACHOSM_RS06055"
                     /old_locus_tag="pgaptmp_001211"
     CDS             2972..3220
                     /locus_tag="ACHOSM_RS06055"
                     /old_locus_tag="pgaptmp_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU4557778.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            3220..4392
                     /locus_tag="ACHOSM_RS06060"
                     /old_locus_tag="pgaptmp_001212"
     CDS             3220..4392
                     /locus_tag="ACHOSM_RS06060"
                     /old_locus_tag="pgaptmp_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGU4557779.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4428..5507)
                     /gene="lptG"
                     /locus_tag="ACHOSM_RS06065"
                     /old_locus_tag="pgaptmp_001213"
     CDS             complement(4428..5507)
                     /gene="lptG"
                     /locus_tag="ACHOSM_RS06065"
                     /old_locus_tag="pgaptmp_001213"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGU4557780.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(5504..6574)
                     /gene="lptF"
                     /locus_tag="ACHOSM_RS06070"
                     /old_locus_tag="pgaptmp_001214"
     CDS             complement(5504..6574)
                     /gene="lptF"
                     /locus_tag="ACHOSM_RS06070"
                     /old_locus_tag="pgaptmp_001214"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGU4557781.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALYVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(6605..7165)
                     /locus_tag="ACHOSM_RS06075"
                     /old_locus_tag="pgaptmp_001215"
     CDS             complement(6605..7165)
                     /locus_tag="ACHOSM_RS06075"
                     /old_locus_tag="pgaptmp_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGU4557782.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(7177..8001)
                     /locus_tag="ACHOSM_RS06080"
                     /old_locus_tag="pgaptmp_001216"
     CDS             complement(7177..8001)
                     /locus_tag="ACHOSM_RS06080"
                     /old_locus_tag="pgaptmp_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557783.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            complement(8001..9350)
                     /locus_tag="ACHOSM_RS06085"
                     /old_locus_tag="pgaptmp_001217"
     CDS             complement(8001..9350)
                     /locus_tag="ACHOSM_RS06085"
                     /old_locus_tag="pgaptmp_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HGU4557784.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQMVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPASLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9396..10154)
                     /locus_tag="ACHOSM_RS06090"
                     /old_locus_tag="pgaptmp_001218"
     CDS             complement(9396..10154)
                     /locus_tag="ACHOSM_RS06090"
                     /old_locus_tag="pgaptmp_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557785.1"
                     /translation="MDALQTQTVISTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(10186..10899)
                     /gene="yghS"
                     /locus_tag="ACHOSM_RS06095"
                     /old_locus_tag="pgaptmp_001219"
     CDS             complement(10186..10899)
                     /gene="yghS"
                     /locus_tag="ACHOSM_RS06095"
                     /old_locus_tag="pgaptmp_001219"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4557786.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGRYLRSKAAHVHEKPSTPPGNITALVICLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            11073..11765
                     /gene="yghT"
                     /locus_tag="ACHOSM_RS06100"
                     /old_locus_tag="pgaptmp_001220"
     CDS             11073..11765
                     /gene="yghT"
                     /locus_tag="ACHOSM_RS06100"
                     /old_locus_tag="pgaptmp_001220"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4557787.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPSAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLIDAEK
                     AITDFMTARGYH"
     gene            complement(11819..13318)
                     /gene="pitB"
                     /locus_tag="ACHOSM_RS06105"
                     /old_locus_tag="pgaptmp_001221"
                     /pseudo
     CDS             complement(11819..13318)
                     /gene="pitB"
                     /locus_tag="ACHOSM_RS06105"
                     /old_locus_tag="pgaptmp_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
     gene            complement(13610..15469)
                     /gene="gss"
                     /locus_tag="ACHOSM_RS06110"
                     /old_locus_tag="pgaptmp_001222"
     CDS             complement(13610..15469)
                     /gene="gss"
                     /locus_tag="ACHOSM_RS06110"
                     /old_locus_tag="pgaptmp_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HGU4557788.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            15674..16540
                     /gene="yghU"
                     /locus_tag="ACHOSM_RS06115"
                     /old_locus_tag="pgaptmp_001223"
     CDS             15674..16540
                     /gene="yghU"
                     /locus_tag="ACHOSM_RS06115"
                     /old_locus_tag="pgaptmp_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGU4557789.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(16663..16911)
                     /gene="hybG"
                     /locus_tag="ACHOSM_RS06120"
                     /old_locus_tag="pgaptmp_001224"
     CDS             complement(16663..16911)
                     /gene="hybG"
                     /locus_tag="ACHOSM_RS06120"
                     /old_locus_tag="pgaptmp_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HGU4557790.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(16924..17265)
                     /gene="hypA"
                     /locus_tag="ACHOSM_RS06125"
                     /old_locus_tag="pgaptmp_001225"
     CDS             complement(16924..17265)
                     /gene="hypA"
                     /locus_tag="ACHOSM_RS06125"
                     /old_locus_tag="pgaptmp_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU4557791.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(17258..17746)
                     /gene="hybE"
                     /locus_tag="ACHOSM_RS06130"
                     /old_locus_tag="pgaptmp_001226"
     CDS             complement(17258..17746)
                     /gene="hybE"
                     /locus_tag="ACHOSM_RS06130"
                     /old_locus_tag="pgaptmp_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HGU4557792.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(17739..18233)
                     /gene="hybD"
                     /locus_tag="ACHOSM_RS06135"
                     /old_locus_tag="pgaptmp_001227"
     CDS             complement(17739..18233)
                     /gene="hybD"
                     /locus_tag="ACHOSM_RS06135"
                     /old_locus_tag="pgaptmp_001227"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HGU4557793.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(18233..19936)
                     /gene="hybC"
                     /locus_tag="ACHOSM_RS06140"
                     /old_locus_tag="pgaptmp_001228"
     CDS             complement(18233..19936)
                     /gene="hybC"
                     /locus_tag="ACHOSM_RS06140"
                     /old_locus_tag="pgaptmp_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HGU4557794.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(19933..21111)
                     /gene="hybB"
                     /locus_tag="ACHOSM_RS06145"
                     /old_locus_tag="pgaptmp_001229"
     CDS             complement(19933..21111)
                     /gene="hybB"
                     /locus_tag="ACHOSM_RS06145"
                     /old_locus_tag="pgaptmp_001229"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HGU4557795.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(21101..22087)
                     /gene="hybA"
                     /locus_tag="ACHOSM_RS06150"
                     /old_locus_tag="pgaptmp_001230"
     CDS             complement(21101..22087)
                     /gene="hybA"
                     /locus_tag="ACHOSM_RS06150"
                     /old_locus_tag="pgaptmp_001230"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HGU4557796.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(22090..23208)
                     /gene="hybO"
                     /locus_tag="ACHOSM_RS06155"
                     /old_locus_tag="pgaptmp_001231"
     CDS             complement(22090..23208)
                     /gene="hybO"
                     /locus_tag="ACHOSM_RS06155"
                     /old_locus_tag="pgaptmp_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HGU4557797.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(23397..23684)
                     /gene="yghW"
                     /locus_tag="ACHOSM_RS06160"
                     /old_locus_tag="pgaptmp_001232"
     CDS             complement(23397..23684)
                     /gene="yghW"
                     /locus_tag="ACHOSM_RS06160"
                     /old_locus_tag="pgaptmp_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HGU4557798.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(23803..24651)
                     /gene="yghX"
                     /locus_tag="ACHOSM_RS06165"
                     /old_locus_tag="pgaptmp_001233"
     CDS             complement(23803..24651)
                     /gene="yghX"
                     /locus_tag="ACHOSM_RS06165"
                     /old_locus_tag="pgaptmp_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HGU4557799.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPARMSGKTPAVV
                     VVHENRGLNPYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLMNDFFAAIEFMQRY
                     PQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADVAKIEAPLLLHFAE
                     LDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDKSAADLAWQRTLKW
                     FDKYLS"
     gene            24808..25848
                     /gene="gpr"
                     /locus_tag="ACHOSM_RS06170"
                     /old_locus_tag="pgaptmp_001234"
     CDS             24808..25848
                     /gene="gpr"
                     /locus_tag="ACHOSM_RS06170"
                     /old_locus_tag="pgaptmp_001234"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HGU4557800.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(25888..26382)
                     /gene="yqhA"
                     /locus_tag="ACHOSM_RS06175"
                     /old_locus_tag="pgaptmp_001235"
     CDS             complement(25888..26382)
                     /gene="yqhA"
                     /locus_tag="ACHOSM_RS06175"
                     /old_locus_tag="pgaptmp_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HGU4557801.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            26573..27457
                     /gene="yghA"
                     /locus_tag="ACHOSM_RS06180"
                     /old_locus_tag="pgaptmp_001236"
     CDS             26573..27457
                     /gene="yghA"
                     /locus_tag="ACHOSM_RS06180"
                     /old_locus_tag="pgaptmp_001236"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU4557802.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(27729..28154)
                     /gene="exbD"
                     /locus_tag="ACHOSM_RS06185"
                     /old_locus_tag="pgaptmp_001237"
     CDS             complement(27729..28154)
                     /gene="exbD"
                     /locus_tag="ACHOSM_RS06185"
                     /old_locus_tag="pgaptmp_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HGU4557803.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(28161..28895)
                     /gene="exbB"
                     /locus_tag="ACHOSM_RS06190"
                     /old_locus_tag="pgaptmp_001238"
     CDS             complement(28161..28895)
                     /gene="exbB"
                     /locus_tag="ACHOSM_RS06190"
                     /old_locus_tag="pgaptmp_001238"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU4557804.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLEVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            29147..30334
                     /gene="metC"
                     /locus_tag="ACHOSM_RS06195"
                     /old_locus_tag="pgaptmp_001239"
     CDS             29147..30334
                     /gene="metC"
                     /locus_tag="ACHOSM_RS06195"
                     /old_locus_tag="pgaptmp_001239"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGU4557805.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            30474..31133
                     /gene="yghB"
                     /locus_tag="ACHOSM_RS06200"
                     /old_locus_tag="pgaptmp_001240"
     CDS             30474..31133
                     /gene="yghB"
                     /locus_tag="ACHOSM_RS06200"
                     /old_locus_tag="pgaptmp_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HGU4557806.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(31173..32072)
                     /gene="yqhC"
                     /locus_tag="ACHOSM_RS06205"
                     /old_locus_tag="pgaptmp_001241"
     CDS             complement(31173..32072)
                     /gene="yqhC"
                     /locus_tag="ACHOSM_RS06205"
                     /old_locus_tag="pgaptmp_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HGU4557807.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCAGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSC
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            32266..33429
                     /gene="yqhD"
                     /locus_tag="ACHOSM_RS06210"
                     /old_locus_tag="pgaptmp_001242"
     CDS             32266..33429
                     /gene="yqhD"
                     /locus_tag="ACHOSM_RS06210"
                     /old_locus_tag="pgaptmp_001242"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HGU4557808.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPADETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            33534..34362
                     /gene="dkgA"
                     /locus_tag="ACHOSM_RS06215"
                     /old_locus_tag="pgaptmp_001243"
                     /pseudo
     CDS             33534..34362
                     /gene="dkgA"
                     /locus_tag="ACHOSM_RS06215"
                     /old_locus_tag="pgaptmp_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
     gene            34562..35488
                     /gene="yqhG"
                     /locus_tag="ACHOSM_RS06220"
                     /old_locus_tag="pgaptmp_001244"
     CDS             34562..35488
                     /gene="yqhG"
                     /locus_tag="ACHOSM_RS06220"
                     /old_locus_tag="pgaptmp_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HGU4557809.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            35539..35796
                     /gene="yqhH"
                     /locus_tag="ACHOSM_RS06225"
                     /old_locus_tag="pgaptmp_001245"
     CDS             35539..35796
                     /gene="yqhH"
                     /locus_tag="ACHOSM_RS06225"
                     /old_locus_tag="pgaptmp_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HGU4557810.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(35839..38058)
                     /gene="ygiQ"
                     /locus_tag="ACHOSM_RS06230"
                     /old_locus_tag="pgaptmp_001246"
     CDS             complement(35839..38058)
                     /gene="ygiQ"
                     /locus_tag="ACHOSM_RS06230"
                     /old_locus_tag="pgaptmp_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HGU4557811.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALLGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVMPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(38169..39581)
                     /gene="ftsP"
                     /locus_tag="ACHOSM_RS06235"
                     /old_locus_tag="pgaptmp_001247"
     CDS             complement(38169..39581)
                     /gene="ftsP"
                     /locus_tag="ACHOSM_RS06235"
                     /old_locus_tag="pgaptmp_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HGU4557812.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(39656..40393)
                     /gene="plsC"
                     /locus_tag="ACHOSM_RS06240"
                     /old_locus_tag="pgaptmp_001248"
                     /gene_synonym="parF"
     CDS             complement(39656..40393)
                     /gene="plsC"
                     /locus_tag="ACHOSM_RS06240"
                     /old_locus_tag="pgaptmp_001248"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HGU4557813.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(40626..42884)
                     /gene="parC"
                     /locus_tag="ACHOSM_RS06245"
                     /old_locus_tag="pgaptmp_001249"
     CDS             complement(40626..42884)
                     /gene="parC"
                     /locus_tag="ACHOSM_RS06245"
                     /old_locus_tag="pgaptmp_001249"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGU4557814.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(43430..43912)
                     /gene="ygiV"
                     /locus_tag="ACHOSM_RS06250"
                     /old_locus_tag="pgaptmp_001250"
     CDS             complement(43430..43912)
                     /gene="ygiV"
                     /locus_tag="ACHOSM_RS06250"
                     /old_locus_tag="pgaptmp_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGU4557815.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNDELTGGRYVVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(43965..44357)
                     /gene="ygiW"
                     /locus_tag="ACHOSM_RS06255"
                     /old_locus_tag="pgaptmp_001251"
     CDS             complement(43965..44357)
                     /gene="ygiW"
                     /locus_tag="ACHOSM_RS06255"
                     /old_locus_tag="pgaptmp_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HGU4557816.1"
                     /translation="MKKFAAVIAVMAVCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRNVNP"
     gene            44509..45168
                     /gene="qseB"
                     /locus_tag="ACHOSM_RS06260"
                     /old_locus_tag="pgaptmp_001252"
     CDS             44509..45168
                     /gene="qseB"
                     /locus_tag="ACHOSM_RS06260"
                     /old_locus_tag="pgaptmp_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HGU4557817.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            45165..46514
                     /gene="qseC"
                     /locus_tag="ACHOSM_RS06265"
                     /old_locus_tag="pgaptmp_001253"
     CDS             45165..46514
                     /gene="qseC"
                     /locus_tag="ACHOSM_RS06265"
                     /old_locus_tag="pgaptmp_001253"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HGU4557818.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            46624..47205
                     /gene="mdaB"
                     /locus_tag="ACHOSM_RS06270"
                     /old_locus_tag="pgaptmp_001254"
     CDS             46624..47205
                     /gene="mdaB"
                     /locus_tag="ACHOSM_RS06270"
                     /old_locus_tag="pgaptmp_001254"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HGU4557819.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            47236..47550
                     /gene="ygiN"
                     /locus_tag="ACHOSM_RS06275"
                     /old_locus_tag="pgaptmp_001255"
     CDS             47236..47550
                     /gene="ygiN"
                     /locus_tag="ACHOSM_RS06275"
                     /old_locus_tag="pgaptmp_001255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HGU4557820.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(47598..49490)
                     /gene="parE"
                     /locus_tag="ACHOSM_RS06280"
                     /old_locus_tag="pgaptmp_001256"
     CDS             complement(47598..49490)
                     /gene="parE"
                     /locus_tag="ACHOSM_RS06280"
                     /old_locus_tag="pgaptmp_001256"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGU4557821.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(49519..50100)
                     /gene="yqiA"
                     /locus_tag="ACHOSM_RS06285"
                     /old_locus_tag="pgaptmp_001257"
     CDS             complement(49519..50100)
                     /gene="yqiA"
                     /locus_tag="ACHOSM_RS06285"
                     /old_locus_tag="pgaptmp_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HGU4557822.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(50100..50927)
                     /gene="cpdA"
                     /locus_tag="ACHOSM_RS06290"
                     /old_locus_tag="pgaptmp_001258"
     CDS             complement(50100..50927)
                     /gene="cpdA"
                     /locus_tag="ACHOSM_RS06290"
                     /old_locus_tag="pgaptmp_001258"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HGU4557823.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(50952..51374)
                     /gene="yqiB"
                     /locus_tag="ACHOSM_RS06295"
                     /old_locus_tag="pgaptmp_001259"
     CDS             complement(50952..51374)
                     /gene="yqiB"
                     /locus_tag="ACHOSM_RS06295"
                     /old_locus_tag="pgaptmp_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HGU4557824.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(51375..52004)
                     /gene="nudF"
                     /locus_tag="ACHOSM_RS06300"
                     /old_locus_tag="pgaptmp_001260"
     CDS             complement(51375..52004)
                     /gene="nudF"
                     /locus_tag="ACHOSM_RS06300"
                     /old_locus_tag="pgaptmp_001260"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HGU4557825.1"
                     /translation="MLKPDNLSVTLGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            52209..53690
                     /gene="tolC"
                     /locus_tag="ACHOSM_RS06305"
                     /old_locus_tag="pgaptmp_001261"
     CDS             52209..53690
                     /gene="tolC"
                     /locus_tag="ACHOSM_RS06305"
                     /old_locus_tag="pgaptmp_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HGU4557826.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            53838..54509
                     /gene="ygiB"
                     /locus_tag="ACHOSM_RS06310"
                     /old_locus_tag="pgaptmp_001262"
     CDS             53838..54509
                     /gene="ygiB"
                     /locus_tag="ACHOSM_RS06310"
                     /old_locus_tag="pgaptmp_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HGU4557827.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            54515..55675
                     /gene="ygiC"
                     /locus_tag="ACHOSM_RS06315"
                     /old_locus_tag="pgaptmp_001263"
     CDS             54515..55675
                     /gene="ygiC"
                     /locus_tag="ACHOSM_RS06315"
                     /old_locus_tag="pgaptmp_001263"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU4557828.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(55713..56501)
                     /gene="ygiD"
                     /locus_tag="ACHOSM_RS06320"
                     /old_locus_tag="pgaptmp_001264"
     CDS             complement(55713..56501)
                     /gene="ygiD"
                     /locus_tag="ACHOSM_RS06320"
                     /old_locus_tag="pgaptmp_001264"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HGU4557829.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTTHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            56644..57417
                     /gene="zupT"
                     /locus_tag="ACHOSM_RS06325"
                     /old_locus_tag="pgaptmp_001265"
     CDS             56644..57417
                     /gene="zupT"
                     /locus_tag="ACHOSM_RS06325"
                     /old_locus_tag="pgaptmp_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HGU4557830.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(57475..57645)
                     /gene="yqiD"
                     /locus_tag="ACHOSM_RS06330"
                     /old_locus_tag="pgaptmp_001266"
     CDS             complement(57475..57645)
                     /gene="yqiD"
                     /locus_tag="ACHOSM_RS06330"
                     /old_locus_tag="pgaptmp_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HGU4557831.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(57906..58559)
                     /gene="ribB"
                     /locus_tag="ACHOSM_RS06335"
                     /old_locus_tag="pgaptmp_001267"
     CDS             complement(57906..58559)
                     /gene="ribB"
                     /locus_tag="ACHOSM_RS06335"
                     /old_locus_tag="pgaptmp_001267"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HGU4557832.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(58663..58811)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            58933..59223
                     /gene="ubiK"
                     /locus_tag="ACHOSM_RS06340"
                     /old_locus_tag="pgaptmp_001268"
     CDS             58933..59223
                     /gene="ubiK"
                     /locus_tag="ACHOSM_RS06340"
                     /old_locus_tag="pgaptmp_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HGU4557833.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        14796 a        15767 c        14800 g        13923 t
ORIGIN      
        1 ttattacaac tgggtacagc atgaaccctc agacagaaaa caaagtgatg agcggctaaa
       61 actggagatt aaggtggcac atatccgcac tcgcgaaaca tatggaaccc ggcggctcca
      121 gacggaactg gcagagaatg gcatcatcgt tggtcgtgac cgactggcac gtcttcgtaa
      181 ggagctaagg ctacgctgta agcagaaacg caagttcaga gcgactacga actcgaacca
      241 caatctgcca gttgcgccaa atctgctgaa ccagacgttc gctcctgcag caccaaatca
      301 ggtctgggtg gcggacctga cgtatgttgc cacacaggag ggatggttgt acctcgctgg
      361 catcaaagat gtttatacgt gcgaaattgt cggctacgcc atgggagagc gcatgacaaa
      421 agagttgaca ggtaaagctc tgtttatggc gctcaggagc cagcgcccac ctgccgggct
      481 aatccaccac tctgatcgag gttcacagta ctgcgcatac gattaccggg tcatacagga
      541 gcagtttggt ctgaaaacat caatgtcgcg taaaggtaac tgttacgaca acgctccgat
      601 ggaaagcttc tggggaacgc tgaaaaatga gagcctgagc cactatcgtt ttaataaccg
      661 ggatgaagcc atctcagtaa tacgggaata cattgagatt ttctacaatc gtcagcgtcg
      721 tcactctcgt ctggggaata tctccccggc agccttcagg gaaaaatatc atcagatggc
      781 tgcttaaaaa aagaacaaat ggtagtgtcc gctattgcca gtacacctca cagttccgaa
      841 cgatatcgcc tacctccagt acacctccgg cagcacccgt tttccccgtg gcgtcattat
      901 cacccatcgc gaagtgatgg ctaatctacg tgctataagc cacgatggca ttaaattacg
      961 ccctggcgac cgctgcgtct cctggctgcc tttctaccat gatatgggac tggtcggctt
     1021 tctcctgacc cccgtcgcca cgcaactttc agtagattat ttgcgcactc aggattttgc
     1081 catgcgtcct ctgcaatggc ttaaattgat cagtaaaaat cgcggcaccg tttccgttgc
     1141 gccgccgttt ggctatgaat tgtgccagcg ccgcgtgaat gaaaaagatc tcgctgaact
     1201 ggatctttcc tgctggcgcg tcgctggtat tggtgctgag ccgatctccg cagaacaact
     1261 ccatcaattc gctgaatgtt tccgtcaggt taactttgac gataaaacgt tcatgccgtg
     1321 ctacggactg gcagaaaatg cgctggctgt cagcttctct gatgaaggct ctggggttgt
     1381 ggttaacgaa gtggatcgcg acatcctaga atatcagggc aaagccgtcg cgccgggtgc
     1441 tgaaacacgc gccgtatcga ctttcgtcaa ctgcggcaaa gcgttgccgg aacatggcat
     1501 tgaaatccgc aatgaagcag gtatgccggt cgcggaacgt gtggtaggcc atatttgcat
     1561 ctccggcccc agcctgatga gcggttactt tggcgaccag gtttcgcaag acgagattgc
     1621 cgcgacgggc tggttagaca ccggcgacct cggttatctg tatgtcaccg gacgtattaa
     1681 agatctgatt attattcgtg gccgtaatat ctggccgcag gatattgaat atattgcgga
     1741 acaagaaccg gaaattcatt ctggcgatgc gattgctttt gttaccgtcc aggaaaaaat
     1801 cattttgcag atccagtgtc ggatcagcga cgaagaacgt cgcgggcagc ttatccacgc
     1861 gctggcggca cggatccaaa gcgaatttgg cgtaaccgcg gctatcgatc tgttgccgcc
     1921 ccacagtatt ccccgaacgt cctccggcaa gcctgcccgt gcggaagcga aaaaacgtta
     1981 tcagaaggct tatgctgcca gtcttaatgt gcaggaatcc ctggcatgaa tcaaactgtc
     2041 gcggtgacgg gcgctaccgg gtttatcggt aaatatatta tcgataacct gctcgcccgc
     2101 ggctttcacg ttcgcgcatt gacgcgtact gcccgcgctc acgtcaatga taatctcacc
     2161 tgggtgcgcg gttcgctgga agatactcat tcacttagcg agctggttgc cggagccagc
     2221 gcagtggtcc attgcgccgg gcaagtgcgc gggcacaaag aagagatttt cacccgctgt
     2281 aacgttgacg gcagcctgcg cctgatgcaa gcagcaaaag agagctgctt ttgccaacgt
     2341 tttctgttta tctcttcgct ggcggcgcgc catcccgagc tctcctggta cgcaaattcc
     2401 aaacacatcg ccgaacaacg gctgactgca atggctgatg aaattacgct gggcgttttt
     2461 cgcccgacag ccgtgtatgg tcccggcgat aaagagttaa aaccgctgtt tgactggatg
     2521 ctgcgcggcc tgctgccacg acttggtgca ccagatacac agctctcttt cctgcacgtc
     2581 accgattttg cgcaagcagt gggtcagtgg ttaagcgccg aaactgtaca gacgcaaacc
     2641 tatgaattat gcgatggcgt cgctggcggc tatgactggc aacgcataca gcaacttgcc
     2701 gccaacgtcc gttgcggttc cgtgcgaatg gttggtattc ctctgccggt actcacctgc
     2761 cttgcggata tcagtaccgc gttgagtcgc ctggcaggta aagaacctat gctgacccgc
     2821 tcgaaaattc gtgaattaac ccacgccgac tggtcggcaa gtaataaccg tatttctgaa
     2881 gatattaatt ggtttcccgg gattagcctg gaacacgcat tacgcaacgg gctattttga
     2941 ttgtcttgtt taaaggtgtg ttaacgtttc tatggtaaat cgtgaaatag taatggatta
     3001 catcctttcc tgtttacagg atttggtcga aaacggtgtg gaaattaagc ccgacagcga
     3061 tctcgtcaac gatcttggcc tggaatcgat caaagtgatg gatctcttaa tgatgctgga
     3121 agacagattc gatatttcca ttcctattaa tattctgctg gatgtcaaaa cccctgcgca
     3181 attaatggaa actctacttc catggctgga gaataaataa tggggctata cgataaatat
     3241 gcgcgccttg ctggcgagcg actgcaattt agtgacaacg ggttaacacc gtttggcacc
     3301 tgtattgatg aagtgtattc cgccaccgaa ggccggatcg ggaataaaaa agtgattctg
     3361 gccgggacca ataattatct cggtttaacc tttaatcatg atgccatcag cgaaggtcag
     3421 gccgcgttag cggcccaagg taccggcacc accggttctc gtatggcaaa cggcagttat
     3481 gcgccgcacc tggcgttaga gaaagagatt gccgagtttt tcaatcgccc taccgctatc
     3541 gttttttcaa ccggttatac cgctaattta ggcgtcatca gcgcacttgc cgaccataac
     3601 gccgtggtgt tactggatgc cgacagccac gccagtattt atgacgcctg ttctctcggt
     3661 ggcgcggaaa ttattcgttt ccgccataac gatgctaaag atctggaacg gcgcatggtt
     3721 cgcctcggtg aacgggcgaa agaggcgatt atcatcgttg agggcatcta cagcatgctc
     3781 ggtgacgttg cgccgctggc ggaaattgtt gatatcaaac gtcgtctcgg tggttatttg
     3841 attgtcgatg aagcgcattc gtttggcgtg ttaggcgcaa cggggcgcgg actggctgaa
     3901 gctgtcggcg ttgaagatga tgtggatatt atcgttggta ctttcagtaa aagtctggcc
     3961 tcgattggcg gttttgctgt cggttcggaa gcgatggaag tgctgcgcta cggcagccgc
     4021 ccgtatattt ttaccgcctc tccttctccg tcctgcattg cgaccgtgcg ctcatcgtta
     4081 agaaccattg ccacacaacc agagctgcgg caaaagctga tggataacgc caaccatctg
     4141 tatgacggtc tgcaaaaact gggttatgag ttaagttcac atattagccc ggtagtgcca
     4201 gtaataatcg gttcgaaaga ggaaggttta cgcatctggc gcgagcttat ctcgctgggc
     4261 gtgtatgtga atcttatcct gccgcctgcc gcgcctgcag gcatcacgct gctgcgctgt
     4321 agcgtaaatg ctgcccacag tcgtgagcaa atcgatgcca tcattcaggc atttgctact
     4381 ctgaagcagt aatgtgagcc cgccgtggtg tttgcgccac ggcgggttta atgttgtctg
     4441 tcataccaat acaccaggct caacgccacc aacacaaacg ctaccggtaa accgagcgcg
     4501 gtaacaggtg cgcttaacgc aaatagcaat cccaaattga ccatgatttg atagctgatc
     4561 caggtgagta agcccacgat gacgcctaca gcgagacggc tacccatccc aggcgaacgc
     4621 ggggcagtaa aggtaaacgg cacagccagc aaaatcatcg ccagggtcag gatgggttgc
     4681 cccagttttt cccacagtgc aaggcggtat tcgctgctgg gttgcccggt ttcctgcaaa
     4741 taatggatgt aatgattaag ctgacggaca gaaaaagtat tccccggcat tgataactct
     4801 tcaagattca tactggtgaa ggccgattgc caggcaagat tatctaatgt ttccagcgtt
     4861 tctttgccat tcagccattt tttatgattc acaccatgca ggacccacgt tttgtcgtct
     4921 tcaatggtgg cactgcgtgc ataaatgtag gattcaagcg agagatcgtc gcgataatga
     4981 aatatctcca cgcccaccgg ctggccctgc tcgttcagcg atttcaccgt cacaaattca
     5041 ttaccacgcc tggcccacag catattgccg gtaatgtcat cgtcctcccc caacgcggtg
     5101 gcggtcgatt tgatttgcaa cgcctgttgc tgcaatggcg acgccaccca ctcatctatc
     5161 gcgcctaacg aaacagtcca caatatcccg gcaaccagcg ccaccagtgc aatacggaag
     5221 atagaaaacc ccgtgctgcg aatggcggta agctcactgt ttttcgataa ctgccccagt
     5281 ccaacaatac cacccaataa cgcgataaac ggcgaaagtt cgaccagtgt gcgcggtaag
     5341 gtcattagca ccaccagcac cgcctgagtc cagcgataac cgcccgggct gacaccatcc
     5401 agttcgttaa tcaggttgaa ggtggtaaaa agcggcagca atagccctgc agcggcggca
     5461 aaaccgagaa agagatgacg gattaaatag cgactgaaaa cattcatcgg gaaaatttct
     5521 gcagcaaaga gaagtcacgt gcgaccagta tcagcaggcc cattagcatg agtccgggca
     5581 ctaaccacaa accaggaatg aggggaatcg cgccgttagc caccagcgta cggcagacgt
     5641 cgccgccata aaaaatggca ataaacagca acgtcagtgg caataacgtt gaaaatcgcc
     5701 cttgccgcgg ttttacccga cttaatgaaa tggccagcag cgccatcaac aatgcggtca
     5761 ggccacggct ttgtcgccat tgcagttcgg catgatcggc aggaaaggcg gagcgcgcca
     5821 gctccgtgac cgatttcgct ttgcgtttga cgttcgggct ttgatccagc ggattcagat
     5881 gtaattgcag attacggtag atctgttcgt tgtcgtcacg cccttgatga tcgagaagat
     5941 aggcagtgcc gttgtgcaac ataacggtcg gtttctcggg tgatgggtca accacatcaa
     6001 ccgaacgggc gcggaaaata cgggttcgat tggcagtaga agtataaatc agcgcgtcag
     6061 tcaggcgatt attatcctga tcaaccgtct gcgaaaggat cattcgtccg ttatcgttag
     6121 tgttaaattt ctttgcccgc aactggcgaa catccagctc cgactgcgac tgttgctcca
     6181 gttgataaat ctgcgcataa gcccacggac gaccatacat cgacagaagg gtgacaaaaa
     6241 tacttagcgg tatcgccaga taaagaacgg ctttgtacaa gcgtcccgga ctgccccccg
     6301 ccgcagagat agcggtaatt tccgagtcgg tatacatctg ccctagcgtc acgccaactg
     6361 acacatacag accaacaggt aacaacatct ccagtgcaat cagtactttg tagaaaacga
     6421 tatccagcac aacataaaga gctaatgtcc catttgccgc ttcggtcaga taacgctgtg
     6481 cggagtagct ggcgaaaata aagatcagaa aaccgacgat aatcagcacc agacggcgcg
     6541 taccacgcat gatgtagtgt tcaaccagct tcatctcact aacgccctca atcctttgag
     6601 ttttttattc attgatcagt ttctgcgcca acgcaatgac atgatctacc tcttccggcg
     6661 tcaggctgcc gtcctttgcc cactgaattt gcccggcttt attcagcacc agaatggttg
     6721 aactctgctc cggcaagcgc caggcaacgc gtccctgtcc attgccgtca ataacgaatt
     6781 gcgcccacgg atagcggcgt ttattttttt cgattttgcc aacaacaaaa tagccagtac
     6841 caaagatggc atcatcggtg ttcacaatgg tggtgggctg aaaacgatcc tgcggaaagt
     6901 ttgccgcttc aaccgcttta attaacagtg agttcttttt cttcgccgac ttacgcccgg
     6961 cgatatactg aataatgcgg acttttcccg ctaattgtgt gctgttccac ttttggtagt
     7021 gaatttcgtc gtcgctattc agaagcagct cgccgccttc ctggatataa acaggggtaa
     7081 ccgttttgcc agtgacgaaa ttatgtgcct gcacacccgg ggctaagagc atgataatca
     7141 acgcaataat caaacgagat gacatcattg ctccggctat agcggaaaac gcgaaatcag
     7201 cagctctaag cgctgacgaa tatcgctatc cagcgccaac ggtgcatcat acgactggcg
     7261 gttttccata tcatccgcca ccatgtcgtc gtgtttatct cccatcaggc cattaatggc
     7321 ggcgaggaaa cgggaaatct gtaacgcctc tttattttca ggatcaagcg atcttaaggt
     7381 gtcgtaataa gaaacctgct ggctgatgat ttttcgcaca tcatgaaatt ccctaccggt
     7441 tagtcgcggt ttggcaataa tctctttcag ggtatgcatt tgcttttgcc gataggcaat
     7501 aaaaccgctt acgctttcta actggctatg gcgtaatgaa tagttcacca acgaccagac
     7561 cggagagctt aaataaatcc gtaacaggtt gccgtaatac gacacaatat ttttcttacc
     7621 gttacgaaag ccgtcctgaa aacgccccag aaaaacggtc gttttgccga ataaaaatcc
     7681 ggcctgatgc ttctttaaat acagccgttg cgcaaaacgc aggtgcttgt agcgcgctcg
     7741 catgtattta aattgtttgc gctcatcagc agtcagctca tcgcctttgg cttgtttgcg
     7801 aactaaacgc agtaattcct gacgatcaac gccattgagc cagaattgca ggcataacgc
     7861 gtaattatca tgaatgattt cggtggtaca acttagtgca atagtcggcg gcaaactaac
     7921 ctgtgcatca acgacatcat cttcttcaat cgcaacaaat agacgctcgc tcgcctcaag
     7981 cgggaaatat tttctcatca tcaggatttc tctccggctg cgttataaag caaggaatgg
     8041 cgctgaaaaa atgcctttaa ggttggaatt aacgagagcg ccacgtccag acattgccca
     8101 ccgaagtaag caaacgctgc tcccagcaca ccaaacagat gacagaacat gaacagcaat
     8161 acgatgtagc cgattgaggc gatggtttgc gccacgagaa aagcgcgctg ttttcccgcc
     8221 atcaatagca atgactcctg agggaagccc agcatcgaaa tcacaattgc gcccagcatc
     8281 acctgaatca ggtcatacgc ttcgagatac ttaacgccaa acaccagcga aatgagcggc
     8341 ttgccgacga tcaacaccgc gagcgccacc agaataccga ttccgcccgc cagtaaaccg
     8401 gacttcacac ctaacaacca cggtctggtg gtgcgcggat ctaaacgcat cacctctggg
     8461 taaaaacttt tacccagcaa actcgctggc gttccggcgg cgtcgaagaa tgtcatggcg
     8521 attttaaata acccggcggc agcgggtcct aacacgatcc ccaccaacac tgtgctgcac
     8581 gagttacgcg ccgaccagat ggagtgggca atgttggttg accagacaaa actccacgcg
     8641 cctttaatgt gtcgggcaga ctcaaacaga ttcaatttga aggcgttatg gatatttcgg
     8701 cggcgtaatt cgcgcgcggc aaaccaccag tacatggtgc cgccaaccag attcgacacg
     8761 taccaggcaa taacaaaacc cgcaaaacca aagtcaaaat accaggctac gacgctcccc
     8821 gctgcgcgca gaaaaggttt cgtcgcctgc tgtacagcaa ttaaatcgaa gcgatctacc
     8881 gcacgcagaa tgccggtcgg cgtggaggaa gccattgaag gaatgagcgt gcaatagagc
     8941 gctgccagcc aaaaactttg gtcatccaga cctaatgaat gggaaaggaa tggcagtaag
     9001 gcaatgccac cgacaatcgc caccgcgccg ctgacgatat ccagcgagaa ggaaaatgag
     9061 acgacattgc ggaattgctg cggattattg ttggttaatg ctggtgttcc gtactgaacc
     9121 accatttgcc atgtctgaaa cttaataaaa tcgctgatcg acttggcgta cgattgcaca
     9181 atcaccagta cgccaaacat ggcgggcgtc atccctttac cggcacacga gagcgccaac
     9241 agacccagca aggcgctcac gacattactg gagcctaacc aggcgctatt gcgaataatg
     9301 gtgcgaaacg cgccatctgc aaaccaatgt ttgatgttaa aacccgccaa ttcagcctga
     9361 ccttatcgtt gatagtaaaa agtatcccgc cagccttaag ttaaacttcg gcggtcagaa
     9421 acgatggcaa ccagagaaac cgccttctgt gcctgttcca gcacttcgct gtagggcgct
     9481 ctggaatcaa tctcaagaat ttttgtgccg ttatagccaa tcgtcgacat gacgccgatt
     9541 ttgtcctgca gctcggcata gtcatggtca ggcttgcggg agatggcagt ctcaatatca
     9601 atgcccaggc gaataattaa ttccgggcga tattgcgcca tttgttggta taaacgccgt
     9661 tcgcgctgcg ccagaaacat gctgattttc ccggtcgcac gttcgacgcc aatccccggt
     9721 ccatcataat aaaagcccga aatttcagcc tgcgggaagc gatcgctgac caccagaacg
     9781 ccactttgcg ccagtcgctg aaccttgcgt agattcgcca ttcttcgcag cgagaagcag
     9841 tacataatca ccgccgccca tagcgccgga gatttggttt tcatgctttg ggttttcgag
     9901 gatttggccg ccagtcgccg ttccagccag acgccaacca acggcaatcg tttgattttg
     9961 tcgccgtctt cgccggagag cagccccaga tagcgccgct cggtttgcca gtgttgttgc
    10021 agcgatttca ccaggtctgt ggtcagtgtg gatttaccgg tgccatcaca cccgaccacc
    10081 gcaattagcc ccggaatgta gctgggctgc ggcgcggttg tactaataac agtttgtgtt
    10141 tgtagtgcat ccattcgtta aatcatcccc tatccatttt ggtgatcagg aaagcgcggc
    10201 gtgaattgcg cgtagtgacg cttgcagaat ttcatcggct ggttgccgac catccagttc
    10261 aaggatcttt gcaccattga atgtcagttg cggcgtaacg gcgatttttt cctgtaacgc
    10321 tgccagttgg tggtcaggtt tacgcgcaaa cgccgtttgt tcatcaatgc caagacgaat
    10381 caacaatacg ggcaaataag atgccatcca ttggtacagc ttcagctcgc gctgccttaa
    10441 catttttatc caaccgttac cgcccgtggt ttttgccaat tgcgggccat caaagcgaaa
    10501 acccggcact tcaacctgcg ggtagcggtc ggtgatgagc agaaagcctt gctggctttt
    10561 acacaacatt ttgcgaaact tgtacgcccg ccagcaggaa agcagacaga taaccagagc
    10621 agtaatattg ccaggcggtg ttgagggttt ttcgtgcaca tgtgccgctt tacttcgcag
    10681 ataacgccca caaggtgcgc caataacagg gagctgtgaa atccattcgc caattcgccc
    10741 ggacgattgc ccgagataaa tgtgttctgt tggcattctt gctgccagtt catttaccag
    10801 gcttgccgtg agggtcgatt tacctgaacc atcacatcct acaatggcaa taacacgcac
    10861 tggcgtggaa ttaattattg acatattacg ttgattcacg aaaaacccgg cagtaaataa
    10921 tgtattgaat attacgtggt ccgtgctcag aatatccgtt caaccttatt gtgacaatgg
    10981 ttaattgtga cattgacctg aattacgctt tcataaaaac atattaacca aataaatatt
    11041 tttaatggat atttaaatta aaggatatat tcatgcagtc aataacacct ccattaattg
    11101 ccgttattgg tagcgatggt tcaggcaagt caacggtgtg tgaacatctt attaccgttg
    11161 tcgaaaaata tggtgctgcc gaaagagttc atttaggaaa acaagccgga aatgtcggtc
    11221 gtgcagtgac aaaattaccg ttgatgggaa aatccttaca taaaacaatt gaacgaaatc
    11281 aggtgaaaac agcaaaaaaa ttgccaggac cagttccggc gctggtaatt acagcgtttg
    11341 tcgcccgtcg cttactgcgc tttcgtcata tgcttgcctg tcgtcgtcgc gggttaattg
    11401 ttctaaccga ccgttatcct caggaccaaa ttcctagcgc ttacgatggt acggtgttcc
    11461 cacctaacgt tgaaggtggt cgttttgtct catggctggc aagccaggaa cgtaaagcgt
    11521 ttcactggat ggcgagccat aagcctgatc tggtcatcaa actcaatgtt gaccttgaag
    11581 ttgcctgcgc gcgtaaaccc gaccataaac gggaatcgct ggcgaggaag attgccataa
    11641 cgccacagtt aacctttggt ggtgcacaac tggttgatat cgatgccaat cagccactgg
    11701 aacaggtgct gattgatgca gaaaaagcga ttacggattt tatgaccgcg cgtggttatc
    11761 actagtcaaa aatggaactg cccgatccga tcgccaggac cgggcatttt caggaaggtt
    11821 aaatcaactg caatgctatc cagtacagcc caccagaaag aaaaattgcc gccggtaaag
    11881 taaacaccca tgccatcagg atgctggtta ccgttttacg ctgtaaccca ccgccgtcca
    11941 ccaccatcgt ccctgctacg gcagatgaga gtacgtgtgt tgtagagacg ggcatcccaa
    12001 tataactggc aaggccgata gacactgccg ccgtcatttg tgccgccatg ccttgcgcat
    12061 acgtcatgcc gcgcttacca atcttctcac cgatggtcat tgctacacga cgccagccaa
    12121 tcatggtgcc aatgccgagc gccagtgcta ccgccatgat gatccacacc ggagcgtact
    12181 caatggtgct taacatatcg ctgcgcagtt ttttcagcag gttctggtct tctttactga
    12241 cgcctggcag tttcgccagc ttcgcggagg tatctgagat gcacagcata atgcggcgca
    12301 gctggctgcg ctgactcacg cttaacggct cgtaactttc catattgcct ggcagcatcg
    12361 ttttaacgcg cgcaatcgca tcaaaggtat ttgccggatg acagtgaaac tctgttactt
    12421 acgcgccatc agtcgatgcc gcaggcaatg gaggttccat cgcaatcaac ttctgcggca
    12481 gttcaggatg ctgttgcaga tagtgttcga agttggagac ggcatcgcga gtacgggtaa
    12541 tttcatagcc ggacgcattc atattgacga cgaagccagc aggggcaatc cccaccagta
    12601 ccagcatcac caggccgatc cctttctgcc cgtcgttcgc gccgtgcgaa aacgccacgc
    12661 ccgcagcgga aacaatcagc gcaatacgcg tccagaatgg cggtttacgt ttgccttttt
    12721 tctttttgcg atcttccgga atgcggtgaa tacggtcacg ctttttcgtc ccgctccaga
    12781 aacggcgcag caggaaaatc agaccacccg caatgaccag gccgacgata ggggaaacaa
    12841 tcagcgagga gaaaattttg gtcacttcac gcaggtttaa cgcatccatc accgatgagc
    12901 cggttaacag cgcgttggtt aaaccgatgc cgataatcgc gccaatcaag gtgtgcgaac
    12961 tggaggccgg taaaccgaag aaccacgtcc ccaggttcca tataatcgcc gccagcagca
    13021 tggaaaagac catcgccaga ccgtgggttg accccatatt cagcaacaaa tcggttggta
    13081 acatatggac aatggcatag gcaacgctaa gtccgcccaa taacacacca aaaaagttaa
    13141 aaaatgccgc catcaccaca gcaagttgtg gttgcatggc acgagtataa ataacggttg
    13201 ccaccgcatt cgccgtgtca tgaaaaccat tgattgcttc gtagaacaac acaaatgcca
    13261 gagcaagtaa taacaaaagc cctgtgtata tatcaaggcc aacaaataaa tttagcatat
    13321 attagattac gccattttga atttacgaac ggacgcatta tcagtgactt taacggcatg
    13381 ggcaaagtga aatatcattt tttaacgata aaaagagtgt tatttcttgt gtatcagaca
    13441 ataagcattc attcaaaacc tttagaaata atcaaaacac aggtattcca ctggttaata
    13501 tattcacata tgaaatgaat aaatattaga acatataaat attgaatatt ttgattaatc
    13561 cccgtactga ttattcttca taatcagtac ggggttgcta caacatgtat tactttttca
    13621 ccacaattaa cggttcaata tcactctctt ttttgatgac cagtgattca tcaccgcgca
    13681 aacacgtccc accgtagttg ccgcccacgg tgaaggtaca tacctgaatg tatttaccgt
    13741 ccactttcgg caaacaccac agttgctgat agatgttttt ctgctcggca aatttaccgc
    13801 tggttttgtc cagcacctct tcatgatggc tgaccaggtc gatattgcta ccacagcgac
    13861 cggcaatcgg tttcactgcg taacctgttt tcaccaattc atcattaaca gtgaaatcgg
    13921 tatccagcag gtaacgatgg tgcgggaaca gcgaccagag gatcggcaga atcgctttgt
    13981 tgccggggat caccgtccac agcggctcaa agactagcac ttccgggcgc agcaatacgt
    14041 cgataagacg cacttcgttt tgcggatgac cggtacggat tggcaccgca gcaaactcac
    14101 ggtcgctaac ttcacgaatc tgatcaaacg cggtttccca cgcccaggtt ttccacacgc
    14161 agttaaccag tcgcccttcc ccatcaatca gttgcccggc agcatcccag cccagttcat
    14221 ccaatccacg caagatacgc gtttcaaagc ccgcctggtg cagcgcctgc tccataaact
    14281 gcgcgtgata gttttcctcg atatctttgt cctgcatgat atggacaaac ggacgtgcac
    14341 gactgtgttt ccaggcacca gccaattcgt taatcagccc ttccgccgga ttgaagccgt
    14401 tgcctttata gccctgctcc gcccaacgtt cgaggatcaa gcccgcttcg gtatgacagg
    14461 aggcggagtc ggcgttgtac tcgtaaacct tcaggccacg ctcatccatg cagaaatcca
    14521 tacgaccagt gatcatatgg tgacggcgac gctgccagga gagacgcaaa cgtggccaga
    14581 ggattttcgg gatgtcgaac agcgccagca ggttgtcatc tttcagcacc ttgtcggttg
    14641 cgtgaagata catcaggtgc agctcgttgg tggctttaat tagctcctgc tcggcactct
    14701 cggtaatggt gtagtaatga taaggatcct gattgatcac ctgaccgttg gcctgcacat
    14761 aggcgttttg cagcggatct ttttcatcca gccatttacc gtcaaactgg cctttgtttt
    14821 ccaggcgcgc tccgctgatt ttcagcagct cgcctgcaat ttccggctgc ggtaagctgt
    14881 attcagtatc ttccgtctgg atcatccagc ccagaatggt ggtgtcatca aaagtgtctt
    14941 tcagggtata gcagccgttt tcgaccacca tctccagctc acgcgtccac tgttgccctt
    15001 gcggcaacgg ggaatgaatc acgttctgtt ccgcaatacg gactttgttg ccatgcaatt
    15061 gggtaatgat ggcgacatgg ccagtgtctt taaattcacc gcctttatcc cagataagaa
    15121 gcgcacccgc gaccggcgca cgcggcgagc cgttaggaaa tgcctgcaat ggcaggatgt
    15181 tgtcattaac cacttctcgc aggaagcgca gcgagaaaat ctcccacgcc atacccacgt
    15241 cagtaaagac cacaccgtaa ttcagaaaga gaaaacggcg agcaaattca acgcattgcc
    15301 acttgtggcc catatattcg tcgtcgatat agctacggaa tacggcgtca tcttcgtatt
    15361 cctgcggatc gagagaactg taatctgaag agtagattgc taccccacct ggggcgtagc
    15421 ccaataatgt cccgaacggg gcatcctggc tggtcgttcc tttgctcatc actttacctt
    15481 taacaataca acctaagcag ttggcgtaat tgttgtgctc tgattacctg ctcagcgaca
    15541 ttaaccggac agaggtcaga ctaatcatac acctcatcgc agcggctggc gcaaagggtt
    15601 aaaaaattca cattctgtac agcaagtgac ctgctacact gcttcaacac taccactcag
    15661 aaggcaactc actatgacag acaatactta tcagcccgcg aaagtctgga cgtgggataa
    15721 atccgctggc ggcgcgttcg ccaatatcaa tcgcccggtt tctggtccga cgcatgaaaa
    15781 aacgctgccc gttggcaaac acccattgca actttattcg ctgggaacgc cgaacggtca
    15841 gaaagtaacg attatgcttg aggagctgct ggcgctgggc gttactggtg cagagtacga
    15901 cgcctggctg attcgtattg gcgatggcga tcaattctcc agcggctttg tcgaagtgaa
    15961 cccaaactcg aagatcccgg cgctgcgcga tcatacgcat aatccgccga tccgcgtgtt
    16021 tgaatctggt tcgatcctgc tttatctggc ggagaaattt ggctacttcc tgccgcagga
    16081 tttggcaaag cgtactgaaa cgatgaactg gctgttctgg ttacagggcg cggcaccgtt
    16141 cctcggcggt ggttttggtc acttttacca ttacgcgccg gtgaaaattg agtacgccat
    16201 caatcgcttt accatggaag ccaaacgtct gctcgacgtg ctggataagc aactggcgca
    16261 gcataagttt gttgcgggcg atgagtacac cattgcggat atggcgattt ggccgtggtt
    16321 tggcaatgtg gtgttaggcg gcgtgtatga tgccgcagag tttcttgatg caggtagtta
    16381 taaacacgta caacgctggg cgaaagaagt gggcgaacgt ccggcggtga agcgtgggcg
    16441 tattgttaac cgcaccaacg ggccgctgaa cgagcagttg catgagcgcc atgacgccag
    16501 tgattttgag acaaatacag aagataagcg tcaggggtaa gggttagcgt tcgtcgcagc
    16561 aagccatcca ggccggataa ggcgttcacg ccgcatccgg caatcgagca caatgcctga
    16621 tgcgacgctg ccgcgtctta tcagacctac gaaagctaat catcacgcgc tggtaatgtc
    16681 gtaatccatt tggcgcagtg cgtctaatgt ggctttggct tcatcttcgt cgatgatgct
    16741 catggcaaat ccgacgtgta ccagcaccca ctggcccagt agatcggcag ggttaccttc
    16801 acaaatcagg gcgatattca cgtcgcgctt gataccacat acttcaacct gcgcaagctg
    16861 gtgaatatct tcaccgacag ccagcacctg gcctggaacg ccaatacaca ttattaactc
    16921 cggttattca acttcaatac ttttgacgat cagcgaatcg ccggtatcga cacgcaaccg
    16981 ctcgccgtga cagagcggac actgcgcatc gtgctgatga atctccacca cctggctgca
    17041 atcccagcac caagcctggg cgggtttata gacgatatgt aaatcgcacc cttgcgccac
    17101 cgttccgtgg cagacaattt caaaactaaa acggacggcg ctctcctcaa cgcaggagag
    17161 cgcgccaatt tccagccaca cggcggtgac gcgcttaaca tcgtgctgct ccgcctgccg
    17221 ttggataatt tcaacggcgc tctggcaaag agacaactca tgcattttcg ccactcctgc
    17281 gaccaaacag cagggcgcga cgccctgcgt gtggtacatc cggattcgtg actggcagcg
    17341 aaaggatcat tcgtgcgcag tcatccgtca ggcgttggcc ctcttcaatc gacatgctgt
    17401 gcgaaagcgg cgacatcagc gaacaggaga gatattgcga aacaccgtcc agttcgccaa
    17461 cggtaaaggt catagtgcca tacggcagtt gcagaccaat tttttcactc actttgcgca
    17521 gcggccagag ttgatccggg ccggggaaga taactgcact cagcatccac ggggtgatca
    17581 cacaccccgt ccactgacct tcgaacagcg taaaatcaga aacatacacc ggcattgaag
    17641 gatgcagaaa agagagatcg tgcatcgaac gccgggcaat ttcttcaaac gctgcctgta
    17701 cttgcgcctt cggggaggtc tggaaacctg ctatctcttc agtcatgaat cgcctcccgt
    17761 gggatggctt ccacgccaga ttcacgcagc gcagccagaa cctgctcaag cgcaggttca
    17821 atcattgctt caaccgtcgg cgttaagccg atgtgtggct ccagcgattc cgggatcacg
    17881 ccgaccaagg tcagcttttt cggaaactca ccggtgaagc gcagggccga caatacgtcg
    17941 gccaggccaa gctgatgcgg agagattttg ttggtaaaca acgccggaac ttcttcatcc
    18001 cgcaggatca tcatcgttcc cggcgtgttc tttttcgaca caatggcatc cgcaataatc
    18061 agatgatcgc gatttgccat gtcgccaagc agctccattc ccgccgtgcc gccatcgagg
    18121 atctcaacat aatccggcag aatgtatcgt tgctctaacg cttcgacaat ccgcacaccg
    18181 atggcttcat cggtcagcaa aatattgccg acccctaaga ctaaaatacg cattacagaa
    18241 ccttcactga aaccacttcg ttgccgtcgg catccactac gtgtaccgca caggccatgc
    18301 acgggtcgaa ggagtgaatg gttcgcacca cttccagcgg tttattcgga tcagcaaccg
    18361 gtgtacccac cagcgactgc tcgtaaggac cgacgtcatc attgaagtta cgcggaccag
    18421 agttccaggt tgatggaaca actgcctggt agttgctgat gataccgtct ttgatcacca
    18481 tccagtgaga gagcataccg cgcggcgctt cgaggaagcc aacacctttg aactcacccg
    18541 ttgccggaat gttcggtttc acaaaggtgg tgtgatcgcc tttgccgata ttggtgatca
    18601 gtgcactgta ttggttttgc aggatatcct gcaattcaca gcagtgaacg gtacgaccaa
    18661 taatacggcc cagcgtggag tgcagctgtg ccacttccag cgtgttgcca gtcagtttct
    18721 gataaatcgc aacgatttca ttcagtttgt tctgggtaga ttctcgacct gccgccagtt
    18781 tcaccagcat attagccagc ggacccactt ctaccgtttt gccgtagaaa gtcggtgatt
    18841 tcacccagga atatttcccg tcgtcagacc aaccatcata agccggaatg gtggtacctt
    18901 cccacggcgc ctgtggcgct tcgtctttat accaggagtg cttcgcgctt tcctgaatgc
    18961 ctttgatcag gtattcatcg gaatgagaag tgatcggacg atacgaggac agatccgcat
    19021 tctcaatgta gccgcccggg aacaggaagc tgccgttttt gctgtcggtc gggaattccg
    19081 gcacgctcag gtagttcacc gcacctttac cgcgcgtcag ccattccggg tagaacgcgg
    19141 cgataaccgc ggtatcaacc ttataaacct gctcaacaaa gtcgctcagt ttgtcgatga
    19201 aagacttgat gtacatcagg cgctcaaggt tcaacacgcc caacccgtcg aggttgattg
    19261 ggttcgcgac accacccact gccaggttct gaatgtgcgg cgttttaccg cccagcagcg
    19321 ccacgacgcg gttagcgtca cgctggcact ccagcgcctg caggtagtgc gctaccgcaa
    19381 tcaggttcac ttccggcggc agtttcatcg ctgggtgacc ccagtagcca ttagcgaaaa
    19441 tacccaactg accgctggca accagatctt tgatcttgtt ctgaactttg gtgaactctt
    19501 ccgggctgtt cagatgccag gtcgaaacgc ctttcagcat ttcggaggct ttggttgggt
    19561 cagcttgcag tgcagaagtg atatccaccc agtccagcgc cgaaagctga tagaaatgaa
    19621 cgatatggtc atgcgtggtg tgcgcagcca gaatgatgtt acggatgtat tgcgcgttaa
    19681 ccggaacgtc gatattcagc gcactttctg ccgcacgaac ggaagacagc gcgtgagtgg
    19741 tagtacatac gccacagata cgttgcacaa tcatccatgc atcgcgcgga tcgcggtttt
    19801 tcacgatctc ttccatgccg cgccacatag taccggaagc ccatgctttc gaaacgacgc
    19861 cattttcgat ttcgcaatcg atgcgtaaat gcccctcaat acgggttacc ggatcaatag
    19921 taattctctg gctcatgctt tgctcgcctc atgacgatta tgatcgtttt gttttaaagg
    19981 aggaagtatc ggcagtagac gaatgagtac gatgtaagcg caaatctcaa tagccacaaa
    20041 accaatagaa atcaacagtt cttcccaggt cgggaagtag gcgtaaccgc cgcccgggtt
    20101 gaatgccacc agcgaatagg tcagacgcca ggttgcacaa cctaacagtg cgctcagtgc
    20161 tgacaggaac agcatgcggg aatcattacg cagcttcgcc acacgcagaa cgaccagcgg
    20221 gaagagcatc agcaggactt caatccagaa catcacggag tagaagtcac cggcaaacgc
    20281 taacgacagc ttgtcgcgat agataagctc gccaaagcgc agcacgatga aaatcgccag
    20341 caacacactg atggtgttgg tcagcttgac gaacagactc ttttcatccg gaccgttgcc
    20401 acgcagaccc gcctgcacca gcgaaccttc aaagatgaca atcgagaagc ccatgatgaa
    20461 cgccgtcagc agcgagaaca gcggcaacat ttcatagctc tgccacaacg gatgcacctt
    20521 gtagcccgcc gagatcatca gtgaccccat tgaagactgg tgcatggtcg gcaacagcgc
    20581 gccaagtgca atgatgaaga acatcacctt gttcagacgc ttgagagaaa ccttccagcc
    20641 cagacgttca aacagtgccg gagcaaactc cagtgccatc acgccgatat agatggtcat
    20701 acagaccgcc gtctcgaaca gtaccgagtt cacgttgaag tgacccggaa tgtagaagta
    20761 cggcaggttc cagtagcgac ccacgtcgat agtgatcgac aagccaccca gtgagtaacc
    20821 aaacagactc gccaacagcg ccggacgcac cagcggatgg tattgcccac ggttaaagac
    20881 gtataccgcc cacgccagcg cccagccgcc acaggcaaag ccggtgccaa tcagcaggtc
    20941 aaacgcgatc cacacgcccc acgggaagcc gccgttcagg tcagagacag agcccagacc
    21001 gaacaccaga cgcttcacaa tcaggagcat acagatgacg attaacggtc caaaaatcat
    21061 gaccggttta ctgatgattt tgccgcccag cggttgtgga tcatgactca tgatcgtctc
    21121 ctccgtcgtg atggtcgttt ttggtgttgc gacgaaccag cacggttaag cccgccagca
    21181 cagccagtgg tagcatcatg cctttataca gggtgtgttg aatattttcg gaacgcgcac
    21241 cggtagaaag atcgtccagt ttcggcaggt cgagattttc ataaggcaca cccgtcagta
    21301 ccagaacctg agtaccgccg ccctctttct cgccgtacag atgcggataa tatttcggca
    21361 ccgtatgcag gtaagtgtcg ccagatttca gcgtctgacg cggatagtgg tattcgctgc
    21421 caggcttcag cgccagacgt tttttcgcct ccgccatcag ctcttcacgc gtaccgaaaa
    21481 tcaccgcgcc cgccgggcac acttctacgc agccaggcag accgccttta tcgagacgtt
    21541 ccacaccttt ctggttgcac agctcgcact tatgcagcgc accaaacggg ttgttgtagt
    21601 cgtacttcgg cacgttgtac ggacaggcga ccatgcagta acggcaaccg gtgcacacgt
    21661 ctttgtcgta atggacaatg ccggttttcg gatctttttt cagtgcagag accgggcaca
    21721 cagagacaca gttcggatcg acgcagtgca tacactgttt cttaatgtac gcgtagccgt
    21781 tctcctcctg gtctttgttg acccctgtgc cgctggtcca cacctgaatg atgttattgg
    21841 tatacggcga cagtttgtcg ttgttcgacc aggtctgttc cccttgcggg ttacgttcag
    21901 ggaagttgat atcctgacac ttggtgacgc aagcctggca gcctacgcac aaggtcgagt
    21961 cgtacaacat ccccagcgat cccggaattg gcgggcggtt ttcagcagcc gcatgactga
    22021 cagacggcag cgcgcccgtc agcaatgccc cgcaggaggc tgctttaata aaattacgtc
    22081 tgttcacggt tattctcccc gtgagtcagc gttatctttc ttttgctgac gacccagttc
    22141 acgcaccgcc atcacgctga caccggcaac cagcccaacc acaccgccga gcaaaccaat
    22201 agcgcctgca gagacgttgc cgccctcttt agcgttaaca tccggtttct gtgaacgcgg
    22261 agtctgattt tcgacgttgg caagctgatg gatgccttta tggaagccga taccttcttc
    22321 gttacagcca tagcatgggt gaccaatcgc taccggccac acgccgccaa catcgcagaa
    22381 ttgcagcgtt gagcagttgc cgtaagtttc tggcccttta cagccgaggt ggtacaggca
    22441 ccagccttca cggtggcctt catcaccgaa ctctttggca aaacgaccag catcgaagtg
    22501 cgggcgacgt tcgcagtgtt cgtgaatcag acggccatag gcgaaggtcg gacgattttt
    22561 gtcatccagt ttcggcggtt tgccgtaagt gatgatgtgc gcaacggtcg cgaggaagtt
    22621 gtgcgggttc ggcgggcagc ccggaatatt gataacggtt ttgcctggca gaacttcttg
    22681 caggctgact gcgccagttg ggttaactcc agctgcggca acaccgcccc acgcagagca
    22741 ggaaccgata gcgataatcg ctgctgcgcc ttctgccgct ttgcggatgt gatccacaat
    22801 cggctcacca gcaaccatgc aataaatacc gttatctttt aatgggatgg aaccatccac
    22861 aaccaacaca tactgccctt tgtacttctc aagtgcgtta tgtttgttct cttcgacctg
    22921 atgaccgaag gcggcggaaa gcacttcgtg atactccaga gagatagtct ccagcacgag
    22981 gttttctacc gttggatgcg ttgcacgaag cagagattcc gtacaaccgg tgcactcctg
    23041 cgcgccaatc cagataactg gcggacgctg cgggttagta accgattcgg ccatctctgc
    23101 agcggctttg ctacttaacc ccatggtggc ggctaatgct gcacaaagct tcatgaaatc
    23161 acgacggtta atgccgtgag aatggatgag ggtgttatct ccagtcattt atagttattc
    23221 cgttgcgaag acctggcata tattttgcct caatcgcaaa atcaataatg cgatcgatgc
    23281 gccatttacc acacgtttat tatggttatc gatatcatga tactgcggcg gggcgctaaa
    23341 acgaagggaa gaagcattag tgtaattaat taaagcaaga taatacgtat gtttgatcaa
    23401 ttttcgaggc gataaccggc cataaaaaag cgcaacgtag aacaggaatt attctcacga
    23461 aagaaatcca ttaccatctc tttatccagt ccatagcggc gcgtcagaat acccgcttcc
    23521 caggcgctaa gctggcgatc tccggtcttt tcgtacatcc ggtgtgagta gcctaataca
    23581 aaaccgcgtt tataatcggc acagaatttt gtcacattaa ccgcactgtc ggcatgcgtt
    23641 gcttttaatc ccgccattaa gcctttacca aaatggttat tcatcgttca cctcattcgc
    23701 tatatatcgc catatatagc gatattttag gcaaagaacg acttttgcgc acgctatttt
    23761 ttcatgcttg tttaagacgt aatccagtaa gagataaacc tatcaggaga gatatttatc
    23821 gaaccatttc agtgtccttt gccaggcaag atcggcggca gatttgtcat aacggggcgt
    23881 ggaatcatta tggaatccgt gattaacccc cggatagata tacgcctcat aaaccttatt
    23941 attggctttc aacgccgcct cgtaagcagg ccagccctcg ttgattcggg tgtccagttc
    24001 cgcgaagtgg agtagtaaag gcgcttcaat cttcgccaca tcggcagtgg gtgcctgacg
    24061 accataaaac ggcaccgcgc aggccagttc cggatacgcg acagccgccg cgttcgatac
    24121 gccaccgcca tagcaaaatc cggtaatacc cactttgcct gccgcttgcg gatagcgttg
    24181 cataaactca attgcggcaa agaaatcatt catcagtttg gttggatcaa cctgctgttg
    24241 cagctcacga cctttatcat cattacccgg ataacctcca acggaactta agccgtcagg
    24301 tgccagggcg atatacggat tcagtccacg attctcatgc accaccacca cggctggcgt
    24361 tttgccgctc atccttgcgg gtttcaccag ataaccccgt acctcgccgt gaccatttgg
    24421 cgaaggatac gtgatgtact cagcaacaat ctccgggtcg gtaaactcta cctgagtcgc
    24481 cagcgcataa tttggcttga gcaaatcaaa caacgctaat gccgtcatcc cgcctaccgc
    24541 atacttcgcc gcaaggttga ggaactcacg tttcgagatt ttcccgtgag cgtaatagtc
    24601 gtagtaatcc agcaactctt gtgggaaatc tttggcggtt aaacgcggca tcgttgcact
    24661 cctcagttgg tgttttttta agcaaagcat aagcacgtgt ttttgcccag tttttcgtca
    24721 ctctgtgagc cagactacgg gatacgcgct ggcgaatcgc taaactagaa acattgtttc
    24781 gaaattgaac ggtggaaagg agaggtcatg gtctggttag cgaatcccga acgttacggg
    24841 cagatgcaat accgctattg cggaaaaagc ggtttacgcc tgcccgcgtt atcgctcggt
    24901 ttatggcaca atttcggtca cgttaacgcg ctggaatcac agcgtgcgat cctgcgtaaa
    24961 gcgtttgatt tgggcattac gcactttgat ttagccaaca attacgggcc gcctccagga
    25021 agcgcagaag agaactttgg tcgcctgctg cgggaggatt ttgccgctta tcgcgatgaa
    25081 ctgattatct ctaccaaggc tggctacgat atgtggcccg gcccttacgg ctctggcggt
    25141 tcacgtaaat acctgctcgc cagcctcgac caaagcctga agcgtatggg gcttgagtat
    25201 gtcgatatct tttactctca tcgcgtcgat gaaaatacgc cgatggaaga aaccgcctct
    25261 gcgctggctc atgcggtaca aagcggtaag gcgctgtatg tcgggatctc ctcttactcg
    25321 ccagagcgga cgcaaaaaat ggtcgagttg ctgcacgagt ggaaaattcc gcttttaatt
    25381 catcaacctt cgtacaattt actgaaccgc tgggtggata aaagcggcct gctggatacc
    25441 ctgcaaaata acggcgtggg ctgtattgcc tttactcctc tggctcaggg attgctgacc
    25501 ggaaaatatc tcaacggcat tccgcaagat tcacggatgc atcgtgaagg gaataaagtt
    25561 cgtggtctga cgccgaaaat gcttaccgaa gccaacctca acagcctgcg cttattgaat
    25621 gaaatggcac agcagcgtgg acaatcaatg gcgcaaatgg cgttaagctg gttgctgaaa
    25681 gatgatcgcg tgacgtcggt attgattggt gccagccgcg cggagcaact agaggagaac
    25741 gtgcaggcgc tgaataatct gacatttagc accgaggagc tggcgcagat tgatcagcat
    25801 atcgccgatg gcgagctgaa tctgtggcag gcgtcttccg ataaatgacc tgttaataac
    25861 gggcgcgcga accgcgcccg aatcagatca gtgattatga cgagtcagtc ggtcaagata
    25921 gcccatcaca aatgcagaga gcacaaacgt cagatggata atgacgtacc acatcagttt
    25981 gttatccggg acatttttcg catccataaa gacgcgcagt aagtgaatgg aagaaattgc
    26041 cacaatcgac gctgctactt tgtttttcag cgacgttgcg tccattttcc ccagccagtt
    26101 cagcttctct ttgttctcgg agatatccag ctgcgagacg aaattctcat aaccggaaaa
    26161 catcaccatc accagcaaac cgccaaccag cgtcatatcc accagcgaca gcaacacgag
    26221 gatcaaatct gattccgcca tcgagaagat attcggcagt acgtgaataa tctcctggaa
    26281 gaacttcagc gccagggcaa ctaacgccag cgaaaggcca aagtacacgg gggcaagcag
    26341 ccagcgagaa gcatacattg cattttcaag aaaacgttcc atacattcct gtcgtgtagt
    26401 taaaacgggc aacagtatat cgcaattgag taaacagcac gcaacagcaa atcaacatat
    26461 acgttaaacc tttgtcgcat aattctgagg tttagggatc ttcctggttc ccttatcaac
    26521 gcatcgagag cgtctaccct taaaagacgt tgaacctgaa gggagaaaaa caatgtctca
    26581 tttaaaagac ccgactacgc agtattacac tggtgaatat cccaaacaga aacaaccgac
    26641 gccaggcatc caggcaaaga tgacaccggt accggattgc ggcgagaaaa cctatgttgg
    26701 tagcggtcgc ctgaaagatc gtaaagcact ggtgacaggg ggcgattccg gaatcggtcg
    26761 cgctgccgcc atcgcttacg cgcgtgaagg ggctgacgtg gcgatcagtt atcttcccgt
    26821 ggaagaagaa gacgcccagg atgtgaaaaa gatcattgaa gaatgcggtc gcaaagccgt
    26881 tctgctgcca ggcgatttaa gcgatgagaa attcgcccgt tcgctggttc acgaagcgca
    26941 caaggcgtta ggcgggctgg atattatggc gctggtcgcc gggaaacagg ttgccattcc
    27001 tgatattgca gacctcacca gcgaacagtt tcaaaagacc tttgccatta acgttttcgc
    27061 gctgttctgg ctaacccagg aagcgatccc cctgctaccg aaaggtgcaa gtatcatcac
    27121 cacttcgtca atccaggcat accagccaag tccgcattta ctggactatg cggctacgaa
    27181 ggcggcgatt ctgaactaca gccgtggcct ggcaaaacag gtcgcggaga aaggtattcg
    27241 ggtgaatatt gtcgcgccag gccctatctg gacggcactg caaatttctg gcggacaaac
    27301 gcaggataag atcccgcagt ttggtcagca aacgccgatg aaacgtgcgg ggcaaccggc
    27361 ggaactggcc cctgtatatg tttatctggc aagtcaggag tcgagctacg tcaccgcaga
    27421 agtgcacggc gtgtgcggcg gcgagcattt aggttaaaaa aatgcccggt tgtgaaaagc
    27481 aaccgggcat cattgtgaat tcactcttac ctgatgtggc gaaaacgcct tatccgccta
    27541 caaacgcatg aagattcaac atgctgcaat agattttgta ggcctgataa gcgtagcgca
    27601 tcaggcattg agcacctaat gccggatgcg tcgtgaacgc cttatccggc ctacaaacgc
    27661 acgaagattc aacatgttgc aatagatttt gtaggcctga tgagcgtagc gcatcaggca
    27721 attctacttt acttcgcttt ggcggtttct tcgccgacca gacctatctt caggtaaccc
    27781 gcctgatgca gcgtatccat taccttcatc aacgtctcgt aatcgacggt tttgtccgct
    27841 cggaagaaga tggtggtgtc tttcttgcct tcggttaacg cattcaacgc cgtaatcatt
    27901 gtttcatcgg tgaccggatc gttaccgata aacatcgagt tgtctgcctt caccgacaga
    27961 taaaccggtt tttccgggcg cggctgcggc gtgctggtag aagcaggcaa gttcaccttc
    28021 acatctaccg tcgctaacgg tgccgccacc ataaagataa tcagcagaac caacatcacg
    28081 tcgataaacg gcgtcacgtt gatatcatgc atttcgccgt tatcgtcgag gttttcgtta
    28141 agatgcattg ccatcggata ttatcctgcg cgtaattttt gtgcgacacg aaccggatgc
    28201 gcagcggcgc tggcttccag atccaggtca cggctttgca gcaacaatac ctgcgctgca
    28261 acatcaccca gcatcgcttt aaagccgcca atctggcgtg caaatacgtt atagataacg
    28321 accgcaggaa tcgctgccac gaggccgatt gccgttgcta acagagcttc tgcgataccc
    28381 ggcgcaacga cttccaggtt agtggtctgc gtttgcgcga taccaataaa gctgttcatg
    28441 atgccccaga ccgtaccaaa cagaccaacg aacggagaaa tcgcgccgat ggttgccaga
    28501 tagccgttac cgcggcccat ttgacgaccc actgcggcga cccgacgctc cagacggaag
    28561 ctggtacgtt ctttaatacc ttcgttatcg tcgctgcctt ctgacagttc cagctcattc
    28621 tgcgcttcat tgagcaaatg caggcttaag cttttgctac caaaatcagc ggcgatatcg
    28681 ttggcctggt ttaaggaacg cgcttcagcc agcagttgct gctcgcgctt aaggcgacgc
    28741 ttctgattga agaactctac gctcttactg aagaagattg cccaggtgac tacggaggcc
    28801 aaaataagcc caatcatcac gcacttaacg acaatatcgg cgtgctgata cataccccag
    28861 acggaaaggt ccgtctgcat taaattatta cccactgtgt atctccagga cgcaagtcac
    28921 aaaatctgcg cataataata tcaaaacgac gtcgaattga tagtcgttct cattactatt
    28981 tgcatactgc cgtacctttg ctttcttttc cttgcgttta cgcagtaaaa aagtcaccag
    29041 cacgccattt gcgaaaattt tctgctttat gccaattctt caggatgcgc ccgcgaatat
    29101 tcatgctagt ttagacatcc agacgtataa aaacaggaat cctgacatgg cggacaaaaa
    29161 gcttgatact caactggtga atgcaggacg cagcaaaaaa tacactcttg gcgcggtaaa
    29221 tagcgtgatt cagcgcgctt cttcgctggt atttgagagt atggaagcca aaaagcacgc
    29281 gacgcgcaat cgcgccaatg gcgagttgtt ctatggacgg cgcggaacgt taacccattt
    29341 ttccttacaa caagcgatgt gtgaactgga aggtggcgca ggctgcgcgc tatttccctg
    29401 cggggcggca gcggttgcta attcgattct tgcttttgtc gaacagggcg atcatgtgtt
    29461 gatgaccaac accgcctatg aaccgagtca ggatttctgt agcaaaatcc tcagcaaact
    29521 gggcgtaacg acatcgtggt ttgatccgct gattggtgcc gatatcgtta agcatctgca
    29581 gccaaacact aaaatcgtgt ttctggaatc gccaggctcc atcactatgg aagtccacga
    29641 cgttccggcg attgttgccg ccgtgcgcag tgtggtgccg gatgccatca ttatgatcga
    29701 caacacctgg gcagccggtg tgctgtttaa ggcgctggat tttggcattg atgtttctat
    29761 tcaggccgcc accaaatatc tggttgggca ttcagatgcg atgattggca ctgcggtgtg
    29821 caatgcccgt tgctgggagc agctacggga aaatgcctat ctgatgggcc aaatggtcga
    29881 tgcagatacc gcctatataa ccagccgtgg tctgcgtact ttgggtgtgc gtttgcgtca
    29941 acatcatgaa agtagcctga aagtagctga atggctggcg gaacatccgc aagtagcgcg
    30001 agttaatcac cctgctctgc ctggcagtaa aggccacgaa ttctggaaac gagactttac
    30061 aggcagcagc ggcctgttct cgtttgtgct taagaaaaaa ctcaatgatg aagagctggc
    30121 gaactatctg gataacttca gtttattcag catggcctac tcgtggggcg ggtatgaatc
    30181 gttgatcctg gcaaatcaac cagaacatat cgccgccatt cgcccacaag gcgagatcga
    30241 ttttagcggg accttgattc gcctgcatat tggtctggaa gatgtcgacg atctgattgc
    30301 cgatctggac gccggttttg cgcgaattgt ataacattgc cacttttgga caattttgca
    30361 gacatttaat tgtgaaaagt cttaaattgt tgcgtccggg atcaaggcgt cccggacgaa
    30421 tcaggagtac aataggcaga taaaggctta aacgctgttc cacaggaaag tccatggctg
    30481 ttattcaaga tatcatcgct gcgctctggc aacacgactt tgccgcgctg gcagatcctc
    30541 atattgttag cgttgtttac tttgtcatgt ttgccacgct gtttttagaa aacggcctgc
    30601 tgcccgcctc atttttgcca ggcgacagct tgttgatact ggcaggcgca ttgattgccc
    30661 agggggttat ggattttctg cctacgattg cgattctgac cgccgcagca agtctgggct
    30721 gctggctaag ttatattcag gggcgctggt tagggaatac caaaacggtg aaaggctggc
    30781 tggcacagct tcctgctaaa tatcaccagc gcgccacctg catgtttgac cgccacggtc
    30841 tgctggcgct gctggctgga cgttttcttg catttgtccg tacgctgctg ccaaccatgg
    30901 cgggaatttc cggtctgcca aaccgccgct tccagttttt caactggtta agtggattgc
    30961 tgtgggtcag cgtggtaacc agttttggct atgccttaag tatgattccg ttcgttaaac
    31021 gccatgaaga tcaggtaatg acgttcctga tgatcctgcc aattgccttg ttaaccgctg
    31081 gcttgttagg cacgctgttt gtggtgatta aaaaaaaata ctgtaacgcc tgacgatttt
    31141 ccccgttccc ggctgctgta ccgggaacgt atttaattcc cctgcatcgc ccgcattctt
    31201 gccgcatctt cccccggcgt cacaccgaag taacgtttaa actcacagct aaattgcgat
    31261 gcgctttcat agccgacgcg catcgctgct gcgctggcct tcatgccgtc atggatgatc
    31321 atcatccgcg ccttatgcag acggtaattc ttcaaatact gcaacggcga ggtgctggtg
    31381 acagacttaa aattatggtg gaacgccgat acgctcatgt tggcttctgc cgccagttgc
    31441 tcgacgctca ggttttcggt gtatttattc tcaatccgtt tcagcacgcg gctaatcaga
    31501 ctgaagtgag tctggcgact gaccagcgcc agtaacgcgc cggcgcaagg tccggtcagc
    31561 acgtagtaca gaatttcgcg gatgatctgt ttgccgagaa tacgcgcatc cagtggtcgc
    31621 tccatcacgt cgagtaaccg ctccgccgcg cataaaatct cttctgataa cgtggcggag
    31681 ttaatcccgc tggctgccat cgacggctgg aaatgctcat cttcgccaat gtccatcaac
    31741 agttcctgta actgcaaaat atcgacattg agacgcaacc ctgccagcgg cacctctgac
    31801 gtggcatagg tttcgcactc aaacggcaac ggcaccgtca gcagcaggta ttcattggca
    31861 tcataacgaa acacgcgttc attgatataa ccgattttat gcccggaaaa gagaattatg
    31921 atgccaggct cgtacatcac cggtgtacgt gcgaaaggcg tctcgccata caacaaacgc
    31981 acatcgggca acagttctga caaactattt tctttatttt tcagtttatt aactttatcc
    32041 gccagcaagc ggcaaatctc ttcacgtttc atatcgcgta atttcttagg aataatgcgg
    32101 caatttgatt gtgcgcaatt ttgtagcatt tctccagcac tctggagaaa taggcaagac
    32161 attggcagaa atgagcattg agagccaggg cgctggcgat cacaatgaaa aacatcaggc
    32221 agatcgttct ctgccctcat attggcccag caaagggagc aagtaatgaa caactttaat
    32281 ctgcacaccc caacccgcat tctgtttggt aaaggcgcaa tcgctggttt acgcgaacaa
    32341 attcctcacg atgctcgcgt attgattacc tacggcggcg gcagcgtgaa aaaaaccggc
    32401 gttctcgatc aagttctgga tgccctgaaa ggcatggacg tgctggaatt tggcggtatt
    32461 gagccaaacc cggctgatga aacgctgatg aacgccgtga aactggttcg cgaacagaaa
    32521 gtgactttcc tgctggcggt tggcggcggt tctgtactgg acggcaccaa atttatcgcc
    32581 gcagcggcta actatccgga aaatatcgat ccgtggcaca ttctgcaaac gggcggtaaa
    32641 gagattaaaa gcgccatccc gatgggctgt gtgctgacgc tgccagcaac cggttcagaa
    32701 tccaacgcag gcgcggtgat ctcccgtaaa accacaggcg acaagcaggc gttccattct
    32761 gcccatgttc agccggtatt tgccgtgctc gatccggttt atacctacac cctgccgccg
    32821 cgtcaggtgg ctaacggcgt agtggacgcc tttgtacaca ccgtggaaca gtatgttacc
    32881 aaaccggttg atgccaaaat tcaggaccgt ttcgcagaag gcattttgct gacgctgatc
    32941 gaagatggtc cgaaagccct gaaagagcca gaaaactacg atgtgcgcgc caacgtcatg
    33001 tgggcggcga cgcaggcgct gaacggtttg attggcgctg gcgtaccgca ggactgggca
    33061 acgcatatgc tgggccacga actaactgcg atgcacggtc tggatcacgc gcaaacactg
    33121 gctatcgtcc tgcctgcact gtggaatgaa aaacgcgata ccaagcgcgc taagctgctg
    33181 caatatgctg aacgcgtctg gaacatcact gaaggttccg acgatgagcg tattgacgcc
    33241 gcgattgccg caacccgcaa tttctttgag caattaggcg tgccgaccca cctctccgac
    33301 tacggtctgg acggcagctc catcccggct ttgctgaaaa aactggaaga gcacggcatg
    33361 acccaactgg gcgaaaatca tgacattacg ctggatgtca gccgccgtat atacgaagcc
    33421 gcccgctaag ctttttacgc ctcaaacttt cgttttcggg catttcgtcc agacttaagt
    33481 tcacaacacc tcaccggagc ctgctccggt gagttcatat aaaggaggaa cgtatggcta
    33541 atccaaccgt tattaagcta caggatggca atgtcatgcc ccagttggga ctgggcgtct
    33601 ggcaagcaag taatgaggaa gtaatcaccg ccattcaaaa agcgttagat agtgggttat
    33661 cgctcgattg ataccgccgc ggcctataag aacgaagaag gtgtcggcaa agccctgaaa
    33721 aatgcctcag tcaacagaga agaactgttc atcaccacta agctgtggaa cgacgaccac
    33781 aagcgccccc gcgaagccct gctcgacagc ctgaaaaaac tacagcttga ttatatcgac
    33841 ctctacttaa tgcactggcc cgttcccgct atcgaccatt atgtcgaagc atggaaaggc
    33901 atgatcgaat tgcaaaaaga gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc
    33961 catcacctgc aacgcctgat tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa
    34021 cttcacccgc tgatgcaaca acgccagcta catgcctgga acgcgacaca caaaatccag
    34081 accgaatcct ggagcccatt agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt
    34141 cgcgatctgg cagataaata cggcaaaacc ccggcgcaga ttgttatccg ctggcatctg
    34201 gatagcggcc tggtggtgat cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt
    34261 gatgtctggg atttccgtct cgacaaagac gaactcggcg aaattgcaaa actcgatcag
    34321 ggcaagcgtc tcggtcccga tcctgaccag ttcggcggct aacatgcaaa ttctcccggt
    34381 ggcggtaatg ttccgctacc ggacttttca gaaatcattt attcccctcg cgtcccgccc
    34441 gttgttactc ttccttgttc aggaatgcca aatataagga catcatcatg cagagccgga
    34501 agctcttaaa agaacaactc atctatatcc gggataaacg caacggagag gtgaaaaaca
    34561 gatgaaaata atacttctgt ttttagcagc cctggcaagt tttaccgtac acgcacagcc
    34621 cccctcactg accgtagaac aaacagtccg gcatatttat cagaactata aatcagatgc
    34681 cactgcccct tattttggtg aaaccggaga gcgggcgata acttctgcgc gtattcaaca
    34741 ggcgcttacc ctgaacgaca atcttacgct gccgggcaat attggctggc tggattatga
    34801 tccggtttgt gattgtcagg attttggcga tctggtgcta gaaagcgttg cgataaccca
    34861 aactgacgcc gatcatgccg atgccgttgt gcgctttcgt atctttaaag atgataaaga
    34921 aaagaccacg cagacactga aaatggtggc ggaaaatggt cgttgggtca ttgacgatat
    34981 tgtcagcaat catggcagcg tcttacaagc agttaatagc gagaatgaaa aaacgctggc
    35041 cgctttagct tcgttgcaaa aagaacagcc ggaagccttt gttgccgaac tctttgaaca
    35101 tattgctgat tatagctggc cgtggacgtg ggtggtttcc gactcttacc gccaggcggt
    35161 taatgccttc tataaaacca ccttcaagac ggccaataat cccgatgaag atatgcaaat
    35221 agaacggcaa tttatttacg acaatccgat ctgttttggc gaagagtcgc tattttcacg
    35281 cgttgatgaa attcgagtcc tggagaaaac cgccgattcc gcccgcattc atgttcgttt
    35341 tacgctgacc aatggcaaca acgaagagca agaactggtt ttacagcggc gcgaaggcaa
    35401 gtgggaaatc gctgatttta tccgcccgaa cagcggcagc ctacttaagc agattgaggc
    35461 aaaaaccgcc gccagattaa agcaatgagc tgaattaaat aacaattagc cggaacaata
    35521 aataaaaggg aacactatat gaaaacgatt ttcaccgtgg gagctgttgt tctggcaacc
    35581 tgcttgctca gtggctgcgt caatgagcaa aaggtcaatc agctggcgag caatgtgcaa
    35641 acattaaatg ccaaaatcgc ccggcttgag caggatatga aagcactacg cccacaaatc
    35701 tatgctgcca aatccgaagc taacagagcc aatacgcgtc ttgatgctca ggactatttt
    35761 gattgcctgc gctgcttgcg tatgtacgca gaatgataaa ataatccccg gcagcatgtc
    35821 agttgccggg gatatttttt aacgtccaac cgccgcttta ggccgtttct tcgcaccagc
    35881 attcaccgga cgagattgcg tagacgacgc tttttttgcc gtagcaggcg tctgacgctg
    35941 ggtcgccatc ggcgtatgtt tcgtcaacgc cggacgggta ttgcggttct ggcgacgagc
    36001 ttcacgcatc tcttcaatgg ttggcgcagg cactaagcaa tcgcgacggc tgccaatcag
    36061 atgctttttg cccatcgctt ccagcgcctg gcggattaac ggccagtttg ccggatcgtg
    36121 gtaacgcaac aacgctttat gcaaacgacg ctgtttgtca cccttcggta caaagacgtc
    36181 ttcactctta taaccaatct tcgccagcgg gtttttcccg gtgtaataca tggtggtcga
    36241 gttagccagc ggcgatggat agaagttctg tacctggtcg agacggaaac gatgcttttt
    36301 cagccacagc gccagattca ccatatcttc atcacgcgta ccggggtgcg cggagatgaa
    36361 ataggggatc agatactgct ctttacctgc ctgttttgag taagtatcga acagctcttt
    36421 aaagcggtca tagctgccca tgcccggctt catcatcttc gataacggcc cttcttcggt
    36481 atgttccggg gcaatcttca gataaccgcc gacgtgatgg gtcgccagct ctttgatata
    36541 gcgtggatct tccacggcga tgtcataacg tacgcctgag gcaatcagga tctttttaat
    36601 gcctttcaga tcgcgagcgc ggcgatagag gttgatcgtc ggttcgtggt tagtgtccat
    36661 atgcggacaa atatccgggt aaacgcagga caaacggcga caagtttgct ctgcgcgtgg
    36721 cgatttgcag cgcaacatat acatgttggc agttggccca ccgagatcgg aaatcacgcc
    36781 cgtaaaacct ggaacggtgt cgcggatcgc ttcaatctca ttaatgatcg aatcttccga
    36841 acggctctga ataatgcgcc cttcgtgctc ggtgatagaa cagaaagagc agccgccaaa
    36901 gcagccgcgc ataatgttga ccgagaaacg gatcatttcg taagccggaa tacgggcatt
    36961 gccataggcc ggatgcggca cgcgcttgta cggcagcgca aagacgctgt ccatctcttc
    37021 ggtagaaagc ggaatagcag gcgggttgat ccacacatag cggtcgccat gtttttgcat
    37081 caatgcgcgg gcgcagcctg ggttagtttc gtggtgcaga atacgcgaag catgggcgta
    37141 cagcacttta tcgcccttca ctttctcgaa agaaggcagc aacacgtagg ttttttccca
    37201 cggtttcggg cgcggcggct gcacggttac ggctttggct tcctgctttt tcggcatcac
    37261 cggtttgtta tccgcacacg gcaaatcttc gccatacgga tgcggtatcg ggtcgatttt
    37321 cccaggagta tcaagacgag tagaatccac gccgctccag cctagcaacg cctcttttac
    37381 gataatcgca gtattacgca catcgcggat ttcgctaatt ggctcgccca tcgccagacg
    37441 atgcgccact tccaccagcg gacgctcacc gttaccaaac atcagcatgt cggctttcga
    37501 atccaccagc acggaacggc gcacggtatc ggaccagtaa tcataatgcg cggtacggcg
    37561 cagactagcc tcaataccgc cgaggatcac cggtacatct ttccacgcct ctttacaacg
    37621 ctgggtataa accagtgtgg cgcgatccgg gcgcttaccc gcgacgttat ccggcgtgta
    37681 ggcatcgtca tgacgtaaac ggcgatcggc ggtataacgg ttgatcatcg aatccatgtt
    37741 gccagcagta acaccgaaaa acagattcgg tttacccaga cgcataaagt cgtctttgct
    37801 gctccagtcc ggctgggcga tgatcccgac acgaaagccc tgcgcttcca gcatacgacc
    37861 gcaaatcgcc atcccgaagc ttgggtgatc aacatacgcg tcgccagtaa ccaaaatgat
    37921 gtcgcagcta tcccagccaa gttgatccat ctcttcacga gacatcggca aaaacggtgc
    37981 cggtccaaaa caggcggccc agtactgcgg ccaggagaac aggtcgcgat ccggttggat
    38041 cagggagata gagctcattt tgcttccaga aatgataaaa aaataatcaa aggccgggga
    38101 ttataagccg gaacgaaaga gaaatcgaaa ggtattccat actcgccctc ctcgggcgag
    38161 tatgaagatt acggtaccgg attgaccaaa agttgcccta tcgagccacg gtccgccatt
    38221 tccagcgtct gactgttgaa gtagaacggg aagtgcgccc aggaaggctg accgaaataa
    38281 accagcagct ccacttgtcc gtcaacccaa acagtatctt tccagcctct gtcttccggg
    38341 aacggcatcg cgccattcac gttacggatc tggaacatta cgccttcaat atggaacgct
    38401 tgcggctcgt ccgcgcgtac cgtccagcgt tcccacgttc cttgctgcgc ggtgacatca
    38461 atacggttga cgtcccacag ctgcccatta atacccgggt catcacccag actgatatcg
    38521 cgactgcgaa ttggcgaacc agccatgatt tcagttggca gcaagcgcat cggaagactg
    38581 tctgtgacca gcggcagaag gccggttggg cgcagcgtta gcaccagggt agaaaccaga
    38641 atgctggatg gctcaaagaa gccacgaata cgatcaacaa tgctcgccgc ttcgccacag
    38701 gtgatcgaca cttcatcgcc gttgctcata tccaccagaa tctcgcggcg ctcgcccggt
    38761 gccagcgaaa gttgcttcac cgatacagga gcaggcaaga atccctgatc gccagaaatc
    38821 acatgtaacg ggcgaccatc gctcatctgt agttgatagc gacgagagtt cgacgcgttc
    38881 agcagtcgca agcgcaccca gccacgcgag acttcaacgt acgggctttg tacaccgtta
    38941 accagcagcg tatcaccaac aaagccgccg cttcccggtt cgttgtattc tggcgtacca
    39001 aagttatcca gccgtttatc ctggataatg accggaaaat catccacacc ataatggttg
    39061 gggataggca gcgacttgct gacttcatct tccaccagcc acattccggc aaggccgtta
    39121 tagacctgct gagccgtgcg gttgggagta ttggcgtgat accacagagt agctgcgttc
    39181 tggcgaatgg gcagtacggg tgcccagtca gcgtttggcg acatcatccg tgccggaccg
    39241 cccatcagcg ggcctggtac ctgtagcccg gccaccgtca ttgagacatt ttctgtcagg
    39301 cggttgctgt aaataagctt aacatcgtcg cccttccaga cgcggatagt cggccccagg
    39361 taacgaccat tgattcccca gaccgacgcg cgtgtccctg gcgtaaatga ccagtgcgca
    39421 cgttgtacag tcataaacag cggttgccca cggcgagatt caagtagcgg cggaacgggt
    39481 agcggttgct gttgcccggc tgcgctggcc ttcaggggaa cagcgcctgc acaaagtgca
    39541 atccccgatg cctgaatgaa ctgacgccga ctgagtgaca tatttgctcc atgtaaaact
    39601 ggctaaagta ataccgtgca ggaaagtgtt ccccgcaaaa atacagattg ccgacttaaa
    39661 cttttccggc ggcttcgcgt tctgcgactt ctttatcgag ctcggcgatt ttttgttcca
    39721 ttatcgaacg acaatgggca gccagctcac gaacctgatc tttgccatac tgactgacgt
    39781 caattggcgg cagcatttcg acaatcacca gaccgttgtg cagtcgatta agattaatct
    39841 tattcgaagt tgtagagacg cacacgggaa taatcgggac gcccgccgca attgccgcgt
    39901 gaaatgctcc agtcttgaac ggtagcaggc cgcgaccacg gctgcgggtt ccttccggga
    39961 acatccagat ggaaatacgg cgttttttga aatgattcac gacttccgca atggtgccgt
    40021 gagctttagt gcgattgttt ctgtcgatca ataagttgcc ggttaaccag tacaactgcc
    40081 cgaagaaggg gatccacagc aagctctttt tacctaccgt caccgtcggc ggttgcacaa
    40141 tgttcgatgc tgtcaccatg tcatagttgt tctggtggtt agcgatatag atagcattgc
    40201 cgtagctttc agcatctgca ggtttgcggc actcaacttt caggccgaac agcggcgcaa
    40261 gacggccaaa catatgccca aaggtggcca catgtttcgg gttacgcggg ctgaaaaggc
    40321 agtaaatgga gccgaataca cagactaaga tgctgtaaat cacggtaata ataagacgaa
    40381 agatatatag cataacaccc tctgaaggtc tgagagactg gcattgtacg tcacctgaaa
    40441 tcaggttagg gcattgttaa aagccgctct ctggaaaagc gtattgttaa tcgcaacgca
    40501 tgaataaaca acggtttcaa gggaattttt atcgtttccg ggggttgaat acgctgccgg
    40561 atgacgactt aacgtttcat ccggcgttcc ttgcaagcgg gaggaaacag gccctccccg
    40621 gcatattact cttcgctatc accgctgctg gcacggcgag gagagtcgat ctcaacacga
    40681 tcgatacgct gcaaaccgcg catcaacgta ccgcggcgtc cacgttcgcc agtgactttc
    40741 tgtaactctt ccgggcgcag tttaattttg cgtttcccaa catgaatggt cagcgtgctt
    40801 tgcggcggca gaacgtacaa ttgcgccaga ccatcttctc cacgcgcggc ttctgccgat
    40861 ggaatgttga taatcttgtt gcctttgccc ttcgacagct gcggcagatc gcttaccggg
    40921 aacatcaaca tacggcctgc ctgagtgatt gccagcagca tatcggaagc atcttcaatc
    40981 accaccggcg gcataacatg ggcattttcc ggtaaggtga tcaaagcctt acctgcacgg
    41041 ttacgcgcca ccagatcgtt aaaggtgcag acgaaaccgt aacccgcatc ggaagccatc
    41101 agcagtttct gatcgtcgct ttccatcagc atatggtcaa cggtcgcccc aggcggcaac
    41161 gttaatttgc cggtgagtgg ttcgccctga ccacgcgccg acggcagcgt aatcgggtcg
    41221 atggcatagc tacgaccggt ggaatcaaca aacactaccg gttggttgct cttacctttc
    41281 accgccgctt tgaagctatc acccgcttta taattcaggc ccggcgcgtc gatatcatgg
    41341 cctttagcgc tgcgtaccca gcccatctgc gacagcacaa tggtgacagg ttcagacggc
    41401 agcatgtcgt gctcgctcat cgctttcgct tcttcgcgtt cctgcaacgg cgaacgacga
    41461 tcgtcaccgt aggcttgcgc gtctgcctgc agttctttct tcagcaggtt attcatttta
    41521 cgctcggaag ccaaaatgcc ctgcaactgg tcgcgctctt tttccagctc actctgctca
    41581 ccgcgaatct tcatctcttc cagtttggca agatgacgca gtttcagttc gaggatcgct
    41641 tccgcctggg tttccgtaag gccaaaccgc gacatcagcg ccggtttcgg ttcatcttcg
    41701 ttacgaatga tctcaatcac ttcgtcgatg ttgagaaacg ccaccagcaa accttccagg
    41761 atatgcaggc gcttgaggac tttctccaga cgatagttca gtcggcggcg cacggtatcg
    41821 cgacggaaca ccagccattc ggagaggatt tccagcaggt ttttcaccgc cggacgacca
    41881 tccagaccga tcatattgag gttaatgcga tagctctttt ccagatcggt ggtagcgaag
    41941 aggtggttca tcacctgatc catatccacg cggttggaac gcggcacaat caccagacgg
    42001 gtcgggttct cgtggtcaga ttcatcgcgc agatcgtcaa ccatcggcag ctttttgttg
    42061 cgcatttgcg cagcaatttg ctccagtacg cgcgcacctg aaacctgatg cggcaatgcg
    42121 ctgataacca ccgcgccatc ttctttcttc cacaccgcgc gcatacgcac tgaaccacgt
    42181 ccgttctcgt agattttacg gatctcggcg cgcgaagtga taatttccgc ttcagtcgga
    42241 taatccggcc cctgcacgat atccagcagc tgatcgagcg tggttttcgg ctggtcgatt
    42301 aatgcgattg ccgcctgagc cacttcacgc aggttatgcg gtggaatatc ggtcgccatg
    42361 ccgacggcaa taccggtggt gccgttaagc aaaatgtttg gcagacgggc gggtagcatt
    42421 ttcggctcct gcaaagtgcc gtcgaagttt ggcacccagt cagccgtccc ctgccccagc
    42481 tcgctcaata gcagctcgga atatttcgac aaccgggatt cggtgtaacg cattgccgcg
    42541 aacgatttcg gatcgtccgg cgcgccccag ttcccctgac catcaaccag cggataacgg
    42601 taagagaacg gctgcgccat caggaccatc gcttcataac aggcgctatc gccgtgcgga
    42661 tggtatttac ccagtacgtc accgacggta cgggccgatt ttttaaattt ggcgctggca
    42721 ttcaggccca gttcagacat cgcatacaca atgcggcgct gaacaggttt aagaccatcg
    42781 ccaataaacg gcaacgcacg gtccatgatc acgtacatgg agtagtttaa gtaggcgttt
    42841 tccgtaaatt catgtagcgc aaggcgctct gccatatcgc tcattaattc tgattcctca
    42901 acttattcgc cagccttcaa aggggcaata ttgccggaga tactacatta tctgccgcgt
    42961 cgagtcacag gagatcagcg tgaatagcgc cgccggacgt gctccgacgg cgataaaggc
    43021 atcaatgtgc cttgatatac aactctttgc tgtagacttg atcggcatag agggcagcac
    43081 aaggttgcgc ccatgccaca ccactcggca tgggggtccg ctccgggcga ttcgagaagt
    43141 tgaggttatg cgagtccagg gaagcctaaa gcatcaaagt agtcgactgt aagtatacgg
    43201 ttcagcccag tttaactgtg tctccaccag tgccccgcgc ctcctgccat aagtgcggct
    43261 tgctcatgga tacccccgag acggcttacg gcgcaccgga taataaattt cgcgtataca
    43321 tggtattcct taccggagaa attataagcc caacaacaat acgcctgtgg cattgttcgc
    43381 tcaaacttat cgcgagtgat ttggctcaca ctccggtaaa gattccccat cacgccaacg
    43441 gcacataaac atccgtttcc agtcgctgct ctgtcacccc ttcggcaaga ttggtgtagt
    43501 gaaacagaat cggtgcttta cgcattttct cgccgcttgc aggcagccag tggcgaataa
    43561 tgccccatat cgtgtgtgaa atatcgtcca gctcgccaac gtggcgggcc acgacataac
    43621 gtccaccggt aagttcatca ttgctcacac cataacgatt atcgggaatc ggttcgctaa
    43681 cgctgccgca gatatcaaag cgaaacgctt ccggtgcggt ggtggcaggg tcgtcccagg
    43741 cgacgccaaa agtctggctt tggttaacag gagaaagccc cgtctcttta cgccacatga
    43801 taaatttcgc cacactgtag ttgagcaatt cagggctaca gcggtgcggc aacatcgcca
    43861 cagggattga tgggaaatcg ataatgttta catccagtgt caggtttgtc attttgcctc
    43921 cctgcgaaat tattcccggg aacgcgtccc gggagcggta acaattacgg atttacgttg
    43981 cggatctgtt tgacgtcaat ttcaacagaa ttccagtctt tatcgacttc accctgaatc
    44041 tcaaccgtat ctttcggcgt caccgtcaca ccgttccagc gtttgtggtc gatatcaaca
    44101 ttgatagtac cgctggcatc tttaaacacg tagagatcgt cagagatgcg ttcaacgata
    44161 ttgccgcgca gggttaccca ggtgtcgtca cgcagggatt ttgcgctttc tacagtcgtt
    44221 acgctgccgt tcggcccctg gaatcctccg gcctgacttt gcgttgccga tgggccagaa
    44281 aaaccgccct gctctgctgc catcaccggt gcactgcaca cggccattac tgcgattact
    44341 gctgcgaatt ttttcatgtt tattactccc tttaatgtct gtttccgagc atttaacaag
    44401 atagtcctta acaacttctt aagggaaaaa aataaaattt aatgctgtac agagcgcgtt
    44461 acaacacggt ttactggcag caaatacggt tatcgcaggg atgaaaaaat gcgaatttta
    44521 ctgatagaag atgacatgct gattggcgac ggcatcaaaa ctggccttag taaaatgggt
    44581 tttagtgtcg actggtttac acaaggtcgt cagggaaaag aggcgcttta tagcgcacct
    44641 tatgatgcgg tgatcctgga tttaacctta ccaggcatgg atggtcgcga tattttgcgc
    44701 gaatggcgag aaaaaggtca gcgtgagccg gtactgatcc tgaccgcgcg cgatgcgctg
    44761 gaggaacgtg tagaagggct gcgtctggga gctgacgatt atctgtgtaa accttttgcg
    44821 ttgatagaag tcgccgccag gctggaagct ctgatgcgcc gaaccaacgg ccaggccagc
    44881 aacgagctgc gccacggtaa cgtcatgctt gaccccggca aacgtatcgc cacgctggct
    44941 ggcgaaccct taacactgaa accaaaagaa tttgccctgc tggaattact gatgcgtaac
    45001 gctggtcggg tactgccgcg caaactgatt gaagagaaac tgtatacctg ggacgaagag
    45061 gtcaccagta atgccgttga agtgcatgtg catcatctgc gacgcaaact cggtagtgat
    45121 tttattcgta ccgtgcatgg tattggctac acattaggtg agaaatgaaa tttacccaac
    45181 gtcttagtct gcgcgtcagg ctgacgctaa tctttttaat tctggcctcg gtgacctggc
    45241 tgctttccag ctttgtcgcc tggaaacaaa caacggataa cgtcgatgaa ttgttcgaca
    45301 cccaactgat gctgtttgcc aagcggttaa gtacgctcga tctcaacgaa atcaacgcgg
    45361 cggatcgcat ggcacagacg ccaaataaat taaaacacgg tcatgttgat gacgatgcgc
    45421 tgacctttgc catctttacc cacgacggca gaatggtcct taacgatggc gataacggag
    45481 aagatattcc ctatagctat caacgggaag gttttgctga cgggcaactg gtcggtgaag
    45541 acgatccttg gcgttttgtc tggatgacct cacctgatgg caaatatcgc atcgttgttg
    45601 gccaggaatg ggaataccgt gaagacatgg cgctggcgat tgttgccggg caattgatcc
    45661 cgtggctggt cgcactgccg attatgttaa tcatcatgat ggtactactg ggtcgtgaac
    45721 tcgcgccgct gaacaaactg gcgctggcac tacgtatgcg tgaccctgac tcggaaaaac
    45781 cactaaacgc gactggcgta cccagcgaag tgcgaccact ggttgagtcg ctaaatcaac
    45841 tgttcgcccg cacacatgcg atgatggttc gagaacgacg ctttacctcc gatgcagctc
    45901 acgaactacg tagcccgtta acagcgctga aagtgcaaac cgaagttgcg cagctctctg
    45961 acgatgatcc gcaggcgcgg aaaaaagcac tgctccaatt acattccggg atcgatcgcg
    46021 ctactcgtct ggttgatcaa ctgctcacgc tatcgcggct ggactcactg gataaccttc
    46081 aggacgtcgc ggagatcccg cttgaagatc tcctgcaatc gtcggtgatg gatatttacc
    46141 acacggcgca gcaggcgaaa attgacgtgc gactgacact caatgcccac agcatcaaac
    46201 gcactggaca accgctattg ctaagtttgt tggtgcgaaa ccttctggat aacgccgtgc
    46261 gctacagtcc acagggcagc gtggtggacg tcacgctgaa tgccgataat ttcatcgtga
    46321 gggataacgg ccccggtgtg acaccagagg cactggcgcg aattggcgaa cgcttctatc
    46381 gcccccccgg acaaaccgct accggcagtg gacttgggct atcgattgtc cagcgaatcg
    46441 ccaaattgca tggcatgaat gttgaatttg ggaatgcgga acaaggtgga tttgaggcga
    46501 aggtgagctg gtaagggtaa gacttttgct aaattcgcaa aagactttgc acattttgct
    46561 aatttcaccg taccgctctg tgacgtacta tagtcgacaa acgtctcacc ttgaggttaa
    46621 aaaatgagca acaccctgat tatcaacggc gcgaaaaaat tcgcccactc caatggtcaa
    46681 ctgaacgaca ccctgaccga agtcgcggat ggcacactgc gcgacctcgg gcatgatgtt
    46741 cgcatcgttc gcgccgacag cgactacgat gtcaaagcgg aagtacaaaa ctttctctgg
    46801 gctgatgtgg tgatctggca gatgccaggc tggtggatgg gcgcgccgtg gacagtgaaa
    46861 aaatatattg atgatgtatt caccgaaggt catggaacgc tgtatgccag cgatggccgt
    46921 acccgcaaag atccgtcaaa aaaatacggt tccggcggcc tggtacaggg caaaaaatat
    46981 atgctttctc tgacctggaa cgcaccaatg gaagccttca ccgaaaaaga tcaattcttc
    47041 cacggcgttg gcgtcgacgg tgtgtatctg ccgttccata aagcaaacca attccttggc
    47101 atggaaccgc tgccgacatt tatcgctaat gacgtgataa aaatgcctga tgttccccgc
    47161 tatactgaag aatatcgcaa gcatcttgtg gaaatttttg gttaactaga gctcaggctt
    47221 tagaaggagt taaccatgct taccgtaatc gcagaaatcc gtactcgtcc tggtcaacat
    47281 caccgtcagg cggtattgga tcagtttgct aaaatcgttc caaccgtact gaaagaagaa
    47341 ggttgccacg gctatgcgcc aatggtggat tgcgctgctg gcgtgagttt ccagtctatg
    47401 gcaccggatt ctatcgtgat gattgagcag tgggaaagca tcgcgcacct tgaagcgcat
    47461 ctgcaaaccc cgcacatgaa ggcgtacagc gaagccgtaa aaggcgacgt gctggagatg
    47521 aatatccgca tcctgcaacc tgggatttaa acctgccttg tttgcccggc catcctgacc
    47581 gggcaatgtt ctttccttta aacctcaatc tccgccatgt cgcctttctc ttgcaaccag
    47641 ttgcggcgat cttccgagcg tttcttcgcc agcagcatat ccatcatcgc gtcagtacgc
    47701 tgatcgtctt catcatcgat agtcaactgc accagacggc gagtgttcgg atcaagcgtg
    47761 gtttcacgca attgcatcgg gttcatttcc cccagacctt taaaacgctg gacgttcggc
    47821 ttgcctttct tgcgttttaa ttgctcaagt acgccctctt tctcttcttc cgtcagcgcg
    47881 taataaacct ctttcccgag atcaatacgg tagagcggtg gcagtgcgac gtaaacgtga
    47941 ccgtgtttca ccaacgcgcg gaaatgtttt acaaacaaag cgcagagcag cgtggcaatg
    48001 tgcagaccat cagagtccgc atctgcgagg atacagattt tgccataacg aagctggctc
    48061 agatcgtcgc tgtctggatc gataccgatc gctaccgaaa tatcgtgcac ttcctgcgaa
    48121 gccagcactt cgtcggaaga gacttcccag gtgttaagga tcttaccttt cagtggcatg
    48181 atcgcctgat attcgcgatc gcgcgcctgc ttggcagatc cgcctgcgga gtcaccttcc
    48241 acgaggaaca gttcggtacg gttaaggtcc tgcgcggtac agtcagccag tttgccaggc
    48301 agcgccgggc cgctggtcag ctttttacgc accacttttt tagccgcacg catacgacgc
    48361 tgggcgctgg aaatcgccat ctctgccagc aactccgccg cctgaacgtt ctggttcagc
    48421 cacaggataa aggcatcttt caccacgcca gaaacaaatg ccgcgcactg acgcgaagag
    48481 agacgctctt tggtctgccc ggcaaactgc ggatcctgca tttttactga cagcacatag
    48541 gcgcagcgat cccagatatc ttccgccgac aactttacac cgcgcggcag aatgttgcgg
    48601 tattcacaga actcacgcat cgcgtccaac aggccctgac gcagaccgtt aacgtgcgta
    48661 ccgccctgca tcgttgggat aaggttgacg tagctttcgg tcagcagttc accgccttcc
    48721 ggcagccaca gtagcgccca gtccacagct tcagtatcac cagcgaaatt accgataaac
    48781 ggtttttctg gcagcgtcgg taaaccgttt accgcttccg ccaggtaatc attcagaccg
    48841 tcctgatagc accagcgctg ttcggtgttg ttgatctcat ctttaaaggt aatctcaacg
    48901 cccgggcaca gtactgcttt ggccttcagc acatgcgtca ggcgtgaaac agaaaaacgc
    48961 gggctgtcaa agaaggtttc atccggccag aagtgcacgc tggtaccagt attgcgttta
    49021 ccgcaagtgc cgacaacctg taaatcctgc accttttcgc cattttcaaa ggcgatgtta
    49081 taaacctgac catcgcggcg cacgttaact tctacgcgct tcgacagggc gttaaccacc
    49141 gaaatcccca cgccatgcag gccgccagag aactggtaat ttttgttaga gaatttaccg
    49201 cctgcatgca gacggcaaag aatcagttca accgccggta caccctcttc cgggtgaata
    49261 tccaccggca tcccgcgccc atcgtcaata acttctaacg actggtcagc atgtaagatt
    49321 acatccacgc gttttgcgtg acccgccagt gcttcatcga cactgttatc aataacttct
    49381 tgccccaaat ggttagggcg agtggtatcg gtatacatcc ccggacggcg gcgaaccggc
    49441 tcaagcccgg tgagtacctc aatggcatca gcgttataag tttgcgtcat ggtttaagtt
    49501 agcaattcga gttgatcgtc agagatggtg cagaccaaga aaatcgacga tcgggttgaa
    49561 ataatcttcg aagcccgtga atgcgtggtt gccgccttct atgacagtct ggcggcagga
    49621 agcgtagtac gccaccgcct ggcggtaatc cagcacttca tctcccgtct gttgcagcag
    49681 ccagatcaaa tccggcgctt ccagcgggtc aatctgcatg actttgagat cgtaaatatg
    49741 gcgtgactct agcacatatt gctgcccggt gtaggggttc tcgttctgac cgagatagtc
    49801 cgtcagcagt tcaaacgggc gcaccgccgg gtttaccacc actgcgggca gcataaaaca
    49861 ttgtgacaac caggtggcgt aatatccccc cagtgacgaa ccgacaatac ccagcgaatc
    49921 gccgccatgt tccaggacaa tggattccag cagctctgcc gcgtcggaag gatacggcgg
    49981 caactgcgga atgatcatct caacgtcagg gtgatgttcc gccagccagt tttttaacaa
    50041 gctcgctttt gcagagcgcg gcgagctgtt gaaaccgtgt aaataaagaa gcgtagacat
    50101 cagtagcctt ctgaagcggt atcaggttgg aaacgtgtgt ccgccaggcg atgcacctcg
    50161 gtggtcagcg tgccatcagc atgtaactcg agagtacgcc agccgggcgc gatggtgtcc
    50221 agcgtaaagt tggaacagtg cggcttaaac tgcacacagg tcgacggcgt tgccagcagg
    50281 cggcgaccgt tccagtcgag atccagctcc tgatgaatat gaccgcacag caagtatttg
    50341 acgtgcggaa acttcgccag cacggtatcc agttcgcccg cgttacgcag actgtgttga
    50401 tcgagccaac tacaacccgc aggtagcgga tgatgatgca gcagcagcaa cgtatggcgt
    50461 tctggcgcat cggccagttt acgttccagc cactcaagct gaaactcgct cagctcaccg
    50521 tgcggcacgc caaacacctg gctatccagc aacaggattt gccattgctc accaataaac
    50581 acccgcttcg ccggggagat acccgcatcc tgtaacgcgc tgtacatcgc gggctggaaa
    50641 tcgtggttgc caggcagcca gacgcagggc gcacgaaaac ttgcgatgcc ttcagcgaaa
    50701 tgctgatagg ccgcagagga ttgatcctgc gctaaatcac ctgtcgcgac aatcaggtcg
    50761 aattcgtgct gatgtggacg aatcgcctcc agcaccgcct ggtagctttc ccaggtgttt
    50821 acccctaaca gggcttcgtg cttttgtgca aacaggtgag tatcggtaat ttgtaaaatc
    50881 ctgactctgg cctcaccagc cagaggaagg gttaacaggc tttccaaatg gtgtccttag
    50941 gtttcacgac gctaataaac cggaatcgcc atcgctccat gtgctaaaca gtatcgcaac
    51001 cagtccgcta aaaactgatt aatttgatgc ttttcgtcgc gttgatgcaa ctttttatta
    51061 ggataatcat accgcgcttt gaagcgaaaa atctgctggc ttgaacacac ttcagccacc
    51121 atcgcgtcat gatacagacg caccgtcatt gacggaaggc tccagtaact gatcgcgggc
    51181 gcagtctgtt ctattgtcac tagggtagtg tatcgggtcg attccacaat cgtcagccga
    51241 tattgtgcgt ttgccacctg atagcttaca gtttcgccgg gtgcgtcatt gcgcggtaac
    51301 aaacggcgca attgtgaaaa gttcatctcg cacaggcgca tcatttcagg aaagtcaggt
    51361 gtgtaacgct tcatttatgc ccactcattt tttaacgctt gatgatgcag ctgcagccat
    51421 tgcaaagcga tgaccgacgc tgcgttgtcg attttcccct cttctaccca ctggtatgcc
    51481 tgttcccggc ttaccacatg aacgcgaata tcttcgtttt catcagccag accatgaata
    51541 ccgcttgcgg tcgtggcgtc cacttcgccc accataattg acgaacgctc actggtgcct
    51601 cccgggcttg ccaggaaact taacaccggt ttggtccgtt tgactatcag tcccgcctct
    51661 tcaatcgctt cgcgacgggc aacatcttcc acactttcac cctcttcaat catcccggca
    51721 accatctcca gtagccaggg ggtttcactg gtgtcgtatg cggcaatccg aatctgctca
    51781 atcagcacaa cttcatcacg cactgggtca aagggtagca agactgcggc gtgaccgcgc
    51841 tcaaaaattt cccgccgtac ctcatgactc atttgcccgt tgaataaacg atgacgaaat
    51901 ctataaagat ctaatgaaaa aaagccgcga taaagtgttt ctcgtgcaat aatttctaca
    51961 tcgtttttgc caagtgtaac ggacaggttg tctggcttaa gcattgttaa tgtcctggca
    52021 ctaatagtga attaaatgtg aatttcagcg acgtttgtct gccgtttgag cagtcatgtg
    52081 ttaaattgag gcacattaac gccctatggc acgtaacgcc aaccttttgc ggtagcggct
    52141 tctgctagaa tccgcaataa ttttacagtt tgatcgcgct aaatactgct tcaccacaag
    52201 gaatgcaaat gaagaaattg ctccccattc ttatcggcct gagcctttct gggttcagtt
    52261 cgttgagcca ggccgagaac ctgatgcaag tttatcagca agcacgcctt agtaacccgg
    52321 aattgcgtaa gtctgccgcc gatcgtgatg ctgcctttga aaaaattaat gaagcgcgca
    52381 gtccattact gccacagcta ggtttaggtg cagattacac ctatagcaac ggctaccgcg
    52441 acgcgaacgg catcaactcg aacgcgacca gtgcgtccct gcagttaact caatccattt
    52501 ttgatatgtc gaaatggcgt gcgttaacgc tgcaggaaaa agcagcaggg attcaggacg
    52561 tcacatatca gaccgatcag caaaccttga tcctcaacac cgcgaccgct tatttcaacg
    52621 tgttgaatgc tattgacgtt ctttcctata cacaggcaca aaaagaagcg atctaccgtc
    52681 aattagatca aaccacccaa cgttttaacg tgggcctggt agcgatcacc gacgtgcaga
    52741 acgcccgcgc gcagtacgat accgtgctgg cgaacgaagt gaccgcacgt aataaccttg
    52801 ataacgcggt agagcagctg cgccagatca ccggtaacta ctatccggaa ctggcggcgc
    52861 tgaatgtcga aaactttaaa accgacaaac cacagccggt taacgcgctg ctgaaagaag
    52921 ccgaaaaacg caacctgtcg ctgttacagg cacgcttgag ccaggacctg gcgcgcgagc
    52981 aaattcgcca ggcgcaggat ggtcacttac cgacgctgga tttaacggct tctaccggga
    53041 tttctgacac ctcttatagc ggttcgaaaa ctcgtggtgc cgctggtacc cagtatgacg
    53101 acagcaatat gggccagaac aaagtgggcc tgagcttctc gctgccgatt tatcagggcg
    53161 gaatggttaa ctcgcaggtg aaacaggccc agtacaactt tgttggtgcc agcgagcaac
    53221 tggaaagcgc gcatcgtagc gtcgtgcaaa ccgtacgttc ctccttcaac aacattaatg
    53281 catctatcag tagcattaac gcctacaaac aagccgtagt ttccgctcaa agctcattag
    53341 acgcgatgga agcgggctac tcggtcggta cgcgtaccat tgttgatgtg ttggatgcaa
    53401 ccaccacgct gtacaacgct aagcaagagc tggcaaatgc gcgttataac tacctgatta
    53461 atcagctgaa tattaagtca gccctgggta cgttgaacga gcaggatctg ctggcactga
    53521 acaatgcgct gagcaaaccg gtttccacta atccggaaaa cgttgccccg caaacgccgg
    53581 aacagaatgc tattgctgat ggttatgcgc ctgatagccc ggcacccgtc gttcagcaaa
    53641 catccgcacg cactaccacc agtaacggtc ataacccttt ccgtaactga tgacgacgac
    53701 ggggcttcgg ccccgtctga acgtaagaca acgtaaagat acgggttatc tgccgcattc
    53761 ttcccccttc tcgcttcaat ttcgaccagc catcctctat tctgatgggt atttaccact
    53821 ggtcccggaa gacaaaaatg aaacggacaa aatccatacg ccacgcatcg ttccgcaaaa
    53881 actggagcgc acgccatctg acaccagtcg ctctcgcggt tgccactgtt tttatgctgg
    53941 ctggctgtga aaagagtgat gaaacagtgt ctctctatca aaatgctgac gactgttcag
    54001 ctgcaaaccc aggcaaaagc gccgaatgta ccaccgcgta caacaatgcg ctgaaagaag
    54061 ccgaacgtac tgcgccgaaa tacgccaccc gtgaagactg tgttgctgaa tttggtgaag
    54121 gtcagtgcca gcaagcacca gcccaggctg gcatggcacc agaaaaccag gcgcaggccc
    54181 agcaatccag cgggagtttc tggatgccgc tgatggccgg ttacatgatg gggcgtctga
    54241 tgggcggcgg cgcgggattt gcacagcagc cgctgttctc ctcgaaaaac ccagccagcc
    54301 cggcttacgg taaatatacc gacgcgacgg gtaaaaacta tggcgcagcc cagccaggcc
    54361 gcaccatgac cgtaccgaag acagccatgg caccaaaacc ggcgaccact actaccgtta
    54421 cccgtggcgg ttttggtgaa tctgttgcca aacaaagcac tatgcagcgt agcgcaaccg
    54481 gcacctcttc tcgttcaatg ggtggctgat accgatggaa agagtcagta ttaccgagcg
    54541 cccggactgg cgcgaaaaag cccacgaata cggtttcaat tttcacacca tgtacggcga
    54601 gccgtactgg tgtgaagatg cttactacaa gttgaccctc gcccaggttg aaaagctgga
    54661 agaagtcacc gccgaactgc accagatgtg cctgaaagtg gtggaaaaag tgatcgccag
    54721 cgatgagctg atgaccaaat tccgcattcc aaaacacacc tggagttttg tgcgtcagtc
    54781 atggctgact caccagccat cgctttattc gcgtcttgat ctggcatggg atggcactgg
    54841 cgaacctaaa ctgctggaaa ataacgccga tacaccaacg tcactatacg aggcggcgtt
    54901 ctttcagtgg atctggctgg aagatcagct taacgccggt aacttgccgg agggcagcga
    54961 ccagtttaac agtctgcaag aaaagctaat cgatcgcttt gtcgagttgc gtgaacagta
    55021 tggcttccag ttgctgcatc tcacctgctg tcgcgacacg gtggaagatc gcggaaccat
    55081 tcagtatttg caggactgcg cgacggaagc tgaaatcgct accgagttcc tctacattga
    55141 cgatatcggg ttaggtgaaa aaggtcagtt cacggattta caggatcagg taatttccaa
    55201 cctgttcaag ctgtatccat gggaatttat gttgcgtgag atgttctcca ccaagctgga
    55261 ggatgcaggc gtacgctggc tggaaccggc gtggaagagc attatctcca acaaagcgct
    55321 tctaccgcta ctgtgggaga tgttcccgaa tcacccgaac ctgctgcccg cttattttgc
    55381 ggaagatgat catccgcaaa tggaaaaata tgtggttaaa ccgatcttct cccgtgaagg
    55441 cgcaaacgtg tcgatcattg agaacggcaa aaccattgaa gcagcggaag gtccgtatgg
    55501 cgaagaaggg atgattgttc agcaattcca cccgttaccg aaattcggcg acagctatat
    55561 gctgattggt agctggctgg tgaacgatca acctgccgga attggcattc gtgaagaccg
    55621 tgcgttgatc acccaggata tgtctcggtt ttatccacat atttttattg agtaatcaat
    55681 gatgccggat ggcattcgcc atccggtaat tgttaaccta tctgcaccga cagcatactc
    55741 aggctgccca tttctatacc atcgaccgga atagtaattg gctcctgccc atcccacgca
    55801 cctaagacat acaacaacgg cagatagtgc tccggcgttg ggttcgataa cgcgccacct
    55861 tcatggtcga ggtaattcac cagaggatgc tgctctaccg ccccttgcca cgtcagattc
    55921 gctttcacat actcattaaa cgacatcgcc caaggatatg gtgaactatc accgtgccac
    55981 ttcactgtac gcaggttatg caccacgttg ccactggcga ccaacattat tccttcatct
    56041 cgcagcgctg ccagtttgcg ccccatttcg aaatgccagg cggcaggttt gctactgtcg
    56101 atactcaact gcaccatcgg gatatcggcg tcaggataca tcttaatcag cacgccccag
    56161 gaaccgtggt caaagcccca ggcttcttta tccagcgtca ccgggaccgg cgctaacagc
    56221 tcaaccagat gctgtgccag cacaggcgaa cccggagcag gataatgcgt atcgtacagc
    56281 gcctgcggga agccaccaaa gtcatgagtc gtgggcggcg tctccatcgc ggtcactcct
    56341 gttccacggg taaaccagtg agccgaaacc accacaatcg cttgcgggcg tggcaatgtc
    56401 atccccaact tctgccagct gcgggtatac aaattatctt ccagcacgtt catcggacta
    56461 ccgtgaccta aaaacaatgc tggcatacgt gttgaagaca tgatgatatc cttaactaaa
    56521 ggtgtcattt tgatatcctc acaatacgct tgttcggcgg agtaagaacc cggataacaa
    56581 tgatgatgat catcagttat tttgacgatc tgcctgaagg tgaagattta taaggagttg
    56641 tcgatgtcag tacctctcat tctgaccata ctggcggggg cagccacgtt tattggcgcg
    56701 tttctcggcg ttctcgggca aaaaccctcg aaccgcttac tggcgttttc gctaggattt
    56761 gcggcgggga tcatgttgct catctcatta atggaaatgc ttcctgccgc actagcagct
    56821 gaaggaatgt cgcctgtgtt gggttatgga atgtttatct tcggtctgct tggctatttt
    56881 ggcctggacc gcatgttgcc acatgcgcat ccgcaggatt taatgcaaaa atcggtgcag
    56941 ccgttgccaa aatcgatcaa gcgcacagcc attctgctca ctctcggcat cagtctgcat
    57001 aacttcccgg aagggattgc cacctttgtc acggcgagca gcaacctgga gctgggattt
    57061 ggcatcgcac tggccgtcgc tttgcacaat atccctgaag gtctggcagt ggcaggcccg
    57121 gtttatgcgg caacggggtc taaacgcacc gcgattctgt gggcggggat ttctggacta
    57181 gcagaaattc ttggtggtgt gctggcgtgg ttaatcctcg gtagcatgat ttccccggtg
    57241 gtcatggcgg caatcatggc ggcggttgca ggaattatgg tggcgctctc ggttgatgaa
    57301 ttaatgccgc tcgccaaaga gattgaccct aataataacc ccagctatgg cgtactatgt
    57361 ggaatgtcag tgatgggatt cagtttagtg ctgctacaaa cggcgggaat tggttaaaaa
    57421 caacatcaga agttgtccag cacatggaca acttctgcca gcttcacgaa agccttattc
    57481 taccttttcg gctgtctctt ctctcgtact gtttaagtat ttgttccgcg cttcaagcag
    57541 tgcgtctctg tcctggcgaa acgcccgaca ataacgtttc aaatgcagga aataacccac
    57601 cacaaccaga gcaatcaata caatccagta ccaggcgata aacatccttt tacccttaat
    57661 atttaacaat actaaacatt tagcgcataa atttcacata tcctttttcg gatatattca
    57721 tatggtcgca taataacgga acaattatat gattttgatc ataatgaaaa agaatgcctg
    57781 atattaatga aattttctca tagaaagaga agcggaatta acatcttgca tagcagggaa
    57841 aaataaaaac cgaagcctgc tacaacttcg gtttcttgat taaggcagta actaagcagc
    57901 ggttttcagc tggctttacg ctcatgtgcc tgacggtatg ccaccaggtc ttcaatagtc
    57961 acgagcgcca tattgtgttt attggcaaac tcaatgcact ctggtgcacg cgccatcgtg
    58021 ccatcgtcat tagtcagctc acacagcaca ccagccggtt taaagcctgc cagcgtcatc
    58081 aaatcaatag tcgcttcagt atgaccgcca cgcgtcagta caccacctgc ctgcgcgcga
    58141 agtgggaaaa cgtggcccgg gcgattcaga tcagaaggtt ttgcgccatc ggcaatcgct
    58201 gcgcgaacgg tcgtaatacg atcagcggca gaaacaccgg tagtcacacc ttcagccgct
    58261 tcaatggtca cggtaaaacc ggtgccatag gcgctggtgt tattttctac catcattggc
    58321 agatcaagtt gtttacggcg atcttcagtg atgcacaggc aaacaatacc gctaccgtgg
    58381 cgaatggtca gcgccatctg ctcaacggtc atggtttctg ccgggaagat catatcgcct
    58441 tcgttttcac ggtcttcatc atcaagcacc attacaccgc gtccttcacg cagcgcagcc
    58501 agtgcatttt caacacgttc gaaaggcgta ccaaaagagg aaagtagcgt ctgattcatg
    58561 gtaaaaaaac ctcactaaaa ttatggttac cagaatcagg gcagtcttag gagtggcggc
    58621 atatagccaa aataacgtga gcgggtccat gcccgacaga atcgttactc tctcccatcc
    58681 ggactctaac cgtcggcccc ggaattacac cggatctgct gtcctttgag ttcgcaccca
    58741 aagcgctcgc gggctttcaa ctgagttgat ttaccgccgg tggggaattt cgccccgccc
    58801 tgagaataag cggattcact ataacgctaa tgattagcgg cagcaacgca tagcttcaca
    58861 taattctggt ttatgactta cccttatcgc actacaatgg cactcaacac ctatcagtac
    58921 agggaaacta caatgattga cccgaaaaaa attgagcaaa tcgctcgcca ggttcacgaa
    58981 tcaatgccta aaggaatcag ggagttcggg gaagatgtgg agaaaaaaat ccgccaaacc
    59041 ctacaagcgc agctgacgcg cctcgacctt gtaagccgtg aagagttcga cgtccaaacg
    59101 caagtcctgt tacggactcg tgaaaaactg gcgctgcttg aacaaagaat cagcgaactg
    59161 gaaaatcgca gtactgagat caaaaagcag ccagatccag agactctccc cccaacgctg
    59221 taaacatttg tctttaatgc actgtaaatt atgaattaat cctctaattt acagtgtttt
    59281 ctatta
//
LOCUS       DAZKHC020000017        58226 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZKHC020000017 DAZKHC020000000
VERSION     DAZKHC020000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 58226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.017"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(200..287)
                     /locus_tag="ACHOSM_RS06345"
                     /old_locus_tag="pgaptmp_001269"
     tRNA            complement(200..287)
                     /locus_tag="ACHOSM_RS06345"
                     /old_locus_tag="pgaptmp_001269"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(251..253),aa:Ser,seq:tga)
     gene            714..1832
                     /gene="hyaA"
                     /locus_tag="ACHOSM_RS06350"
                     /old_locus_tag="pgaptmp_001270"
     CDS             714..1832
                     /gene="hyaA"
                     /locus_tag="ACHOSM_RS06350"
                     /old_locus_tag="pgaptmp_001270"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HGU4557834.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTLAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            1829..3622
                     /gene="hyaB"
                     /locus_tag="ACHOSM_RS06355"
                     /old_locus_tag="pgaptmp_001271"
     CDS             1829..3622
                     /gene="hyaB"
                     /locus_tag="ACHOSM_RS06355"
                     /old_locus_tag="pgaptmp_001271"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HGU4557835.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            3641..4348
                     /gene="hyaC"
                     /locus_tag="ACHOSM_RS06360"
                     /old_locus_tag="pgaptmp_001272"
     CDS             3641..4348
                     /gene="hyaC"
                     /locus_tag="ACHOSM_RS06360"
                     /old_locus_tag="pgaptmp_001272"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HGU4557836.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            4345..4932
                     /gene="hyaD"
                     /locus_tag="ACHOSM_RS06365"
                     /old_locus_tag="pgaptmp_001273"
     CDS             4345..4932
                     /gene="hyaD"
                     /locus_tag="ACHOSM_RS06365"
                     /old_locus_tag="pgaptmp_001273"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HGU4557837.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            4929..5327
                     /gene="hyaE"
                     /locus_tag="ACHOSM_RS06370"
                     /old_locus_tag="pgaptmp_001274"
     CDS             4929..5327
                     /gene="hyaE"
                     /locus_tag="ACHOSM_RS06370"
                     /old_locus_tag="pgaptmp_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HGU4557838.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            5324..6181
                     /gene="hyaF"
                     /locus_tag="ACHOSM_RS06375"
                     /old_locus_tag="pgaptmp_001275"
     CDS             5324..6181
                     /gene="hyaF"
                     /locus_tag="ACHOSM_RS06375"
                     /old_locus_tag="pgaptmp_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HGU4557839.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            6315..7859
                     /gene="appC"
                     /locus_tag="ACHOSM_RS06380"
                     /old_locus_tag="pgaptmp_001276"
     CDS             6315..7859
                     /gene="appC"
                     /locus_tag="ACHOSM_RS06380"
                     /old_locus_tag="pgaptmp_001276"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HGU4557840.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFALKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            7871..9007
                     /gene="appB"
                     /locus_tag="ACHOSM_RS06385"
                     /old_locus_tag="pgaptmp_001277"
     CDS             7871..9007
                     /gene="appB"
                     /locus_tag="ACHOSM_RS06385"
                     /old_locus_tag="pgaptmp_001277"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU4557841.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            9020..9112
                     /gene="cbdX"
                     /locus_tag="ACHOSM_RS06390"
                     /old_locus_tag="pgaptmp_001278"
     CDS             9020..9112
                     /gene="cbdX"
                     /locus_tag="ACHOSM_RS06390"
                     /old_locus_tag="pgaptmp_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HGU4557842.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            9192..10490
                     /gene="appA"
                     /locus_tag="ACHOSM_RS06395"
                     /old_locus_tag="pgaptmp_001279"
     CDS             9192..10490
                     /gene="appA"
                     /locus_tag="ACHOSM_RS06395"
                     /old_locus_tag="pgaptmp_001279"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HGU4557843.1"
                     /translation="MKAILIPFLSLLIPLTSQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCTITVHTQADTSSPDLLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTDAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(10605..12785)
                     /gene="etk"
                     /locus_tag="ACHOSM_RS06400"
                     /old_locus_tag="pgaptmp_001280"
     CDS             complement(10605..12785)
                     /gene="etk"
                     /locus_tag="ACHOSM_RS06400"
                     /old_locus_tag="pgaptmp_001280"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HGU4557844.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(12805..13251)
                     /gene="etp"
                     /locus_tag="ACHOSM_RS06405"
                     /old_locus_tag="pgaptmp_001281"
                     /gene_synonym="yccY"
     CDS             complement(12805..13251)
                     /gene="etp"
                     /locus_tag="ACHOSM_RS06405"
                     /old_locus_tag="pgaptmp_001281"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HGU4557845.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(13239..14378)
                     /gene="gfcE"
                     /locus_tag="ACHOSM_RS06410"
                     /old_locus_tag="pgaptmp_001282"
     CDS             complement(13239..14378)
                     /gene="gfcE"
                     /locus_tag="ACHOSM_RS06410"
                     /old_locus_tag="pgaptmp_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HGU4557846.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTTDADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(14424..16520)
                     /gene="gfcD"
                     /locus_tag="ACHOSM_RS06415"
                     /old_locus_tag="pgaptmp_001283"
     CDS             complement(14424..16520)
                     /gene="gfcD"
                     /locus_tag="ACHOSM_RS06415"
                     /old_locus_tag="pgaptmp_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU4557847.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDIAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(16520..17266)
                     /gene="gfcC"
                     /locus_tag="ACHOSM_RS06420"
                     /old_locus_tag="pgaptmp_001284"
     CDS             complement(16520..17266)
                     /gene="gfcC"
                     /locus_tag="ACHOSM_RS06420"
                     /old_locus_tag="pgaptmp_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU4557848.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(17263..17907)
                     /gene="gfcB"
                     /locus_tag="ACHOSM_RS06425"
                     /old_locus_tag="pgaptmp_001285"
     CDS             complement(17263..17907)
                     /gene="gfcB"
                     /locus_tag="ACHOSM_RS06425"
                     /old_locus_tag="pgaptmp_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HGU4557849.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(18014..18319)
                     /gene="gfcA"
                     /locus_tag="ACHOSM_RS06430"
                     /old_locus_tag="pgaptmp_001286"
     CDS             complement(18014..18319)
                     /gene="gfcA"
                     /locus_tag="ACHOSM_RS06430"
                     /old_locus_tag="pgaptmp_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HGU4557850.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(18761..18973)
                     /gene="cspH"
                     /locus_tag="ACHOSM_RS06435"
                     /old_locus_tag="pgaptmp_001287"
     CDS             complement(18761..18973)
                     /gene="cspH"
                     /locus_tag="ACHOSM_RS06435"
                     /old_locus_tag="pgaptmp_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HGU4557851.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            19259..19471
                     /gene="cspG"
                     /locus_tag="ACHOSM_RS06440"
                     /old_locus_tag="pgaptmp_001288"
     CDS             19259..19471
                     /gene="cspG"
                     /locus_tag="ACHOSM_RS06440"
                     /old_locus_tag="pgaptmp_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HGU4557852.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            19482..19670
                     /gene="ymcF"
                     /locus_tag="ACHOSM_RS06445"
                     /old_locus_tag="pgaptmp_001289"
     CDS             19482..19670
                     /gene="ymcF"
                     /locus_tag="ACHOSM_RS06445"
                     /old_locus_tag="pgaptmp_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGU4557853.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHVPLDFTK"
     gene            19645..19875
                     /gene="ymcE"
                     /locus_tag="ACHOSM_RS06450"
                     /old_locus_tag="pgaptmp_001290"
     CDS             19645..19875
                     /gene="ymcE"
                     /locus_tag="ACHOSM_RS06450"
                     /old_locus_tag="pgaptmp_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HGU4557854.1"
                     /translation="MCRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            19865..20038
                     /gene="gnsA"
                     /locus_tag="ACHOSM_RS06455"
                     /old_locus_tag="pgaptmp_001291"
     CDS             19865..20038
                     /gene="gnsA"
                     /locus_tag="ACHOSM_RS06455"
                     /old_locus_tag="pgaptmp_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGU4557855.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(20087..21159)
                     /gene="yccM"
                     /locus_tag="ACHOSM_RS06460"
                     /old_locus_tag="pgaptmp_001292"
                     /pseudo
     CDS             complement(20087..21159)
                     /gene="yccM"
                     /locus_tag="ACHOSM_RS06460"
                     /old_locus_tag="pgaptmp_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(21231..23975)
                     /gene="torS"
                     /locus_tag="ACHOSM_RS06465"
                     /old_locus_tag="pgaptmp_001293"
     CDS             complement(21231..23975)
                     /gene="torS"
                     /locus_tag="ACHOSM_RS06465"
                     /old_locus_tag="pgaptmp_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HGU4557856.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISTHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSASSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAASLVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            24058..25086
                     /gene="torT"
                     /locus_tag="ACHOSM_RS06470"
                     /old_locus_tag="pgaptmp_001294"
     CDS             24058..25086
                     /gene="torT"
                     /locus_tag="ACHOSM_RS06470"
                     /old_locus_tag="pgaptmp_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HGU4557857.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVNIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(25059..25751)
                     /gene="torR"
                     /locus_tag="ACHOSM_RS06475"
                     /old_locus_tag="pgaptmp_001295"
     CDS             complement(25059..25751)
                     /gene="torR"
                     /locus_tag="ACHOSM_RS06475"
                     /old_locus_tag="pgaptmp_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HGU4557858.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            25881..27053
                     /gene="torC"
                     /locus_tag="ACHOSM_RS06480"
                     /old_locus_tag="pgaptmp_001296"
     CDS             25881..27053
                     /gene="torC"
                     /locus_tag="ACHOSM_RS06480"
                     /old_locus_tag="pgaptmp_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HGU4557859.1"
                     /translation="MRKLWNALRRPSACWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            27053..29599
                     /gene="torA"
                     /locus_tag="ACHOSM_RS06485"
                     /old_locus_tag="pgaptmp_001297"
     CDS             27053..29599
                     /gene="torA"
                     /locus_tag="ACHOSM_RS06485"
                     /old_locus_tag="pgaptmp_001297"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HGU4557860.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLSDHVHNAARIRY
                     PMVRVDWLRKRHRSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFQPYLLGEK
                     DGQPKDAAWAEKLTGIDAESIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            29596..30195
                     /gene="torD"
                     /locus_tag="ACHOSM_RS06490"
                     /old_locus_tag="pgaptmp_001298"
     CDS             29596..30195
                     /gene="torD"
                     /locus_tag="ACHOSM_RS06490"
                     /old_locus_tag="pgaptmp_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HGU4557861.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(30288..30593)
                     /gene="cbpM"
                     /locus_tag="ACHOSM_RS06495"
                     /old_locus_tag="pgaptmp_001299"
     CDS             complement(30288..30593)
                     /gene="cbpM"
                     /locus_tag="ACHOSM_RS06495"
                     /old_locus_tag="pgaptmp_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HGU4557862.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(30593..31513)
                     /gene="cbpA"
                     /locus_tag="ACHOSM_RS06500"
                     /old_locus_tag="pgaptmp_001300"
     CDS             complement(30593..31513)
                     /gene="cbpA"
                     /locus_tag="ACHOSM_RS06500"
                     /old_locus_tag="pgaptmp_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HGU4557863.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            31774..33033
                     /gene="yccE"
                     /locus_tag="ACHOSM_RS06505"
                     /old_locus_tag="pgaptmp_001301"
     CDS             31774..33033
                     /gene="yccE"
                     /locus_tag="ACHOSM_RS06505"
                     /old_locus_tag="pgaptmp_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HGU4557864.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSE
                     GITYTIQYVRDIDIVRVSLPGRASESITDFKGYYWYGFMEYIENINACDDVFSECCFD
                     DENISVQPERINTPGISDLDSDIDLSGISFIQRETNQALGLKYAPVDGDGYCLLRAIL
                     VLKQHDYSWALVSYKMQKEVYNEFIKMVDKKTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNQIQGQSSLMSWSFLFFKKQFIDSCLNNEKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLMNTGNHYDVYLPP
                     ELFAQAYKLNNKEMNAQLDYLNRYAIY"
     gene            33324..34579
                     /gene="agp"
                     /locus_tag="ACHOSM_RS06510"
                     /old_locus_tag="pgaptmp_001302"
                     /pseudo
     CDS             33324..34579
                     /gene="agp"
                     /locus_tag="ACHOSM_RS06510"
                     /old_locus_tag="pgaptmp_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
     gene            complement(34617..34844)
                     /gene="yccJ"
                     /locus_tag="ACHOSM_RS06515"
                     /old_locus_tag="pgaptmp_001303"
     CDS             complement(34617..34844)
                     /gene="yccJ"
                     /locus_tag="ACHOSM_RS06515"
                     /old_locus_tag="pgaptmp_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HGU4557865.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(34865..35443)
                     /gene="wrbA"
                     /locus_tag="ACHOSM_RS06520"
                     /old_locus_tag="pgaptmp_001304"
     CDS             complement(34865..35443)
                     /gene="wrbA"
                     /locus_tag="ACHOSM_RS06520"
                     /old_locus_tag="pgaptmp_001304"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000607019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU4557866.1"
                     /translation="MINHILRHVETMARAVAEGASKVDGAEVVVKRVPETMPPQLFEK
                     AGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLA
                     SVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYGATTIA
                     GGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            complement(35440..36750)
                     /locus_tag="ACHOSM_RS06525"
                     /old_locus_tag="pgaptmp_001305"
     CDS             complement(35440..36750)
                     /locus_tag="ACHOSM_RS06525"
                     /old_locus_tag="pgaptmp_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4557867.1"
                     /translation="MANSAYPAGVENHGGKLRITFKYRGKRVRENLRVPDTPKNRKIA
                     GELRASVCFAIRTGTFDYAERFPDSPNLKLFGLVKKDITVGELAQKWLTLKAMEISSN
                     ALNRYQSVMKNMLPRLGPGRLASSITKEDLLFIRKDLLTGEKGSRKTSTSRKGRTVPT
                     VNYYMTTTAGMFSFAAENGYLEKNPFNSITPLRKSKPVPDPLTRDEFSRLIDACHHQQ
                     TKNLWTVAVFTGMRHGEIAALAWEDIDLKAGTITVRRNFTKIGDFTLPKTDAGTNRVI
                     HLLAPAIEALKNQAMLTRLSRQHQITVQLREYGRTILHECTFVFCPQIVRKNHKAGIN
                     YAVSSIGATWDSAIKRAGIRSRKAYQSRHTYACWALSAGANPTFIASQMGHSSASMVY
                     NVYGAWMPECSVTQVAMLNNVLNARAPDVPQSDQEDEIKLYFSK"
     gene            complement(36803..37087)
                     /locus_tag="ACHOSM_RS06530"
                     /old_locus_tag="pgaptmp_001306"
     CDS             complement(36803..37087)
                     /locus_tag="ACHOSM_RS06530"
                     /old_locus_tag="pgaptmp_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4557868.1"
                     /translation="MRELVNQHNHGIQPVITPVVQINANEWVTLELLMAVTGLRKGTI
                     LRARDSAWMNGREYKQIAPDGTPKKNSECLYHLPTINTWIKNQPLPSQDV"
     gene            complement(37133..37384)
                     /locus_tag="ACHOSM_RS06535"
                     /old_locus_tag="pgaptmp_001307"
     CDS             complement(37133..37384)
                     /locus_tag="ACHOSM_RS06535"
                     /old_locus_tag="pgaptmp_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4557869.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIS
                     AFNEEFERIEHNELHMIKAEIEKEKHIASLRKMRRTSIN"
     gene            complement(37749..38102)
                     /locus_tag="ACHOSM_RS06540"
                     /old_locus_tag="pgaptmp_001308"
     CDS             complement(37749..38102)
                     /locus_tag="ACHOSM_RS06540"
                     /old_locus_tag="pgaptmp_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4557870.1"
                     /translation="MNAVLTELNKLGKASAESISKGLNIDLNDVIDTLWKLKNQGVVT
                     VKNGIWQAVAREVDKKPNIAPVQPVQHNIIGDLLRKSRKEARRAGRKQKRWEGACKAL
                     QELNKYRDLINELSE"
     gene            complement(38138..38350)
                     /locus_tag="ACHOSM_RS06545"
                     /old_locus_tag="pgaptmp_001309"
     CDS             complement(38138..38350)
                     /locus_tag="ACHOSM_RS06545"
                     /old_locus_tag="pgaptmp_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU4557871.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARSGIRFVPIPITADAELH
                     LFGEILSRKLDELEKLVEEADTSLTV"
     gene            complement(38310..38936)
                     /locus_tag="ACHOSM_RS06550"
                     /old_locus_tag="pgaptmp_001310"
     CDS             complement(38310..38936)
                     /locus_tag="ACHOSM_RS06550"
                     /old_locus_tag="pgaptmp_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HGU4557872.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAADNCLLSMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS"
     gene            complement(38933..39364)
                     /locus_tag="ACHOSM_RS06555"
                     /old_locus_tag="pgaptmp_001311"
     CDS             complement(38933..39364)
                     /locus_tag="ACHOSM_RS06555"
                     /old_locus_tag="pgaptmp_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HGU4557873.1"
                     /translation="MKQIAFYRRSGRPGAFRGLKERVTWMIQSRGRPVTGSEIAEKFG
                     VSLCEFNKVARGLTKGSKVVKIKASEPFTTDTGIVDRFFSLESNPRRDTPRSRNAVPP
                     FSRRSREHAAKNCREEYVQKAERRRRLIKAGLYIDEFENAL"
     gene            complement(39420..40058)
                     /locus_tag="ACHOSM_RS06560"
                     /old_locus_tag="pgaptmp_001312"
     CDS             complement(39420..40058)
                     /locus_tag="ACHOSM_RS06560"
                     /old_locus_tag="pgaptmp_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HGU4557874.1"
                     /translation="MTSQLIPVFNGTIDNETILLVNARDLHSFLGVGRMFAHWVKERI
                     AEYGFIESQDYILICQNGQTKGRGGDRRSKDYHLTLDTAKELAMVERNEKGRQIRRYF
                     IECEKKLRQSLLPAPMNINYPLSWFSEHHPYSMMSYVDRKTLNLDVSVLFDMPSPTMR
                     ILNELHSKGYNVDAAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
     gene            complement(40414..40695)
                     /locus_tag="ACHOSM_RS06565"
                     /old_locus_tag="pgaptmp_001313"
                     /pseudo
     CDS             complement(40414..40695)
                     /locus_tag="ACHOSM_RS06565"
                     /old_locus_tag="pgaptmp_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309191.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(40861..41310)
                     /locus_tag="ACHOSM_RS06570"
                     /old_locus_tag="pgaptmp_001314"
                     /pseudo
     CDS             complement(40861..41310)
                     /locus_tag="ACHOSM_RS06570"
                     /old_locus_tag="pgaptmp_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628766.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(41313..41504)
                     /locus_tag="ACHOSM_RS06575"
                     /old_locus_tag="pgaptmp_001315"
     CDS             complement(41313..41504)
                     /locus_tag="ACHOSM_RS06575"
                     /old_locus_tag="pgaptmp_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557875.1"
                     /translation="MNGQISIVRPGACDDSEIHMIIRLARGKTITVLTTPENLALALT
                     GKSDLPVELKLRNVEIKVK"
     gene            complement(41506..41913)
                     /locus_tag="ACHOSM_RS06580"
                     /old_locus_tag="pgaptmp_001316"
     CDS             complement(41506..41913)
                     /locus_tag="ACHOSM_RS06580"
                     /old_locus_tag="pgaptmp_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4557876.1"
                     /translation="MSKIDYQALREAAEKAGEDKWQAKKINGDFFVIRHGSYTRQHGY
                     TSYQPIAEIDCKPVRDFVAKANPATVLELLDELEAAKKRIAELEAREILLPERSSMLH
                     RTDFHDDYQTVMAYKVSEVIDAIRATGIRIKGE"
     gene            complement(41910..42635)
                     /locus_tag="ACHOSM_RS06585"
                     /old_locus_tag="pgaptmp_001317"
     CDS             complement(41910..42635)
                     /locus_tag="ACHOSM_RS06585"
                     /old_locus_tag="pgaptmp_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4557877.1"
                     /translation="MTTEIDYQVLRDVSERAITAMTHLSILPGDDDLLSEKKLKELGI
                     DIDAIHAFKIMAGPETVLSLLDERDALNERIAELEANLAKLAEDQQKAIESIKQADSA
                     VKLAHEKFSALAAENAGLNKFIAQSCYVFDGEQDELSDAYICATDGGMPQIPATDAFL
                     AEIRAEARNEGINYTASRLAAAFNHGFINKSLREVFDVTRMILSAKEELANESHPIDG
                     LSGEYAEKSLEEWAERLRKGGNQ"
     gene            complement(42786..43181)
                     /locus_tag="ACHOSM_RS06590"
                     /old_locus_tag="pgaptmp_001318"
     CDS             complement(42786..43181)
                     /locus_tag="ACHOSM_RS06590"
                     /old_locus_tag="pgaptmp_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557878.1"
                     /translation="MQFKDLPADIQKIAADTLKAHLSVLNLIKEPKTNLENISRNVRD
                     VFVGLYAYDNEKHEEHIQNGCINKCQQHIKLPVADKTEQKTELLVFWNTVEAIARSKM
                     FEFNNINHSGIDTHQSSHREGVSMLLALL"
     gene            complement(43258..44079)
                     /locus_tag="ACHOSM_RS06595"
                     /old_locus_tag="pgaptmp_001319"
     CDS             complement(43258..44079)
                     /locus_tag="ACHOSM_RS06595"
                     /old_locus_tag="pgaptmp_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HGU4557879.1"
                     /translation="MSQLDSNAIKEIVKLTTTAFSGENLPLTECPVALLPDNVNIESL
                     ERFMTERFRFRGVMTTTSIDDFVEYSKGYADEHSRCFINAETMKAVTVFNIGTLEQPG
                     HADNKALLELKATSPYRALRDVDGKKQLQKSLAEWLEDWADFLTAYDSDGNVLDIKQA
                     ISAVRRLTIDAKRSAEYEEQNFSGSRSVMESVEAKSKEIMPATFRFECIPYEGLGNRE
                     FTLRLSILTSEQPVLVLRIVRVEAAEEEIAKEFRDLLKERFEEEDILTFIGTFSV"
     gene            complement(44143..44490)
                     /locus_tag="ACHOSM_RS06600"
                     /old_locus_tag="pgaptmp_001320"
     CDS             complement(44143..44490)
                     /locus_tag="ACHOSM_RS06600"
                     /old_locus_tag="pgaptmp_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557880.1"
                     /translation="MNQHQTDVNVFINDLDGGVFVNKLGAVLSEVAFGVNSTNKKGKV
                     CVEFELSSLDENRVSVSHKLKFTRPTMRGSKSEEDTTNTPMFVNKGGELTLFQKDQGQ
                     LFDKQGQHDAVLR"
     gene            complement(44565..45152)
                     /locus_tag="ACHOSM_RS06605"
                     /old_locus_tag="pgaptmp_001321"
     CDS             complement(44565..45152)
                     /locus_tag="ACHOSM_RS06605"
                     /old_locus_tag="pgaptmp_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HGU4557881.1"
                     /translation="MDEVIFTYNEESALTAGQGSFITETGAHIINITEAELKQSEKGA
                     RFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGASIIHAMMGCTGIGQLTQHMVSVSK
                     YVAPEFHGKKIGLVLQKVLTTNRKTGADGYQMEIRIPFIAETGQTLKEKAEGKKPETV
                     ANMVSTLKDKDNRSKNVNPNHTDDPGYWQYGSDSF"
     gene            complement(45152..45841)
                     /locus_tag="ACHOSM_RS06610"
                     /old_locus_tag="pgaptmp_001322"
     CDS             complement(45152..45841)
                     /locus_tag="ACHOSM_RS06610"
                     /old_locus_tag="pgaptmp_001322"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU4557882.1"
                     /translation="MTPEIILQRTGVDITSLDQGDDGWHKLRLGVITASEVHNVIAKP
                     RSGSKWPDTKMSYFHTLLAEVCTGVAPEVNAKSLAWGKQYEDDARALFEFIADVTVSE
                     TPIIFRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFDAIKPAYMAQVQF
                     SMWVTDKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIDKMDEALAEIG
                     FVFGEQWGVNN"
     gene            complement(45838..46788)
                     /locus_tag="ACHOSM_RS06615"
                     /old_locus_tag="pgaptmp_001323"
     CDS             complement(45838..46788)
                     /locus_tag="ACHOSM_RS06615"
                     /old_locus_tag="pgaptmp_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HGU4557883.1"
                     /translation="MFDIVEFVKQQERFFCEALTEPTLTWAKESQFAIQQFQKNAFLA
                     DTARANLPSAQNAIINVAAIGITLNPASKLAYLVPRKKAVCLDISYMGLLHLAQVTGA
                     IQWGQCKLVYEKDIYESNGIDCAPTHKYNPFVDRGARIGGYCVVKTSEGDYLTEEMSN
                     REIEVIRACSKAGNNGGSSPWDSFPDEMARKAIVKRASKYWPRRDRLDTAIDYLNTQG
                     GEGIILNADHIPERDVTPASDEIINEITQAITEINKTWDDLLPLCSKTFRRTIASHEY
                     LSQEEAVKTLDFVKKKAARNKATAEAKIHATTENNSEAVS"
     gene            complement(46805..47086)
                     /gene="gamL"
                     /locus_tag="ACHOSM_RS06620"
                     /old_locus_tag="pgaptmp_001324"
     CDS             complement(46805..47086)
                     /gene="gamL"
                     /locus_tag="ACHOSM_RS06620"
                     /old_locus_tag="pgaptmp_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor GamL"
                     /protein_id="HGU4557884.1"
                     /translation="MNAYLTCDRIEERRWINQQIQDEKDKWIDDRAQELISMFPDKPL
                     LMSSLFLPKEAQLALTGEKAEEAYNDYISAIAYARAEEEWERKFSPCPF"
     gene            complement(47107..47388)
                     /locus_tag="ACHOSM_RS06625"
                     /old_locus_tag="pgaptmp_001325"
     CDS             complement(47107..47388)
                     /locus_tag="ACHOSM_RS06625"
                     /old_locus_tag="pgaptmp_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGU4557885.1"
                     /translation="MLNQKININVKSVVTPAGTIMGEVFMDDKIIAYFVVLSDEAISV
                     IDTEGNVMFIAEHPEDIALQAAAYFFAKEQEEECNCPVCQLSRQINLMH"
     gene            complement(47400..47612)
                     /locus_tag="ACHOSM_RS06630"
                     /old_locus_tag="pgaptmp_001326"
     CDS             complement(47400..47612)
                     /locus_tag="ACHOSM_RS06630"
                     /old_locus_tag="pgaptmp_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HGU4557886.1"
                     /translation="MLKPHYGTALVSREDVKPGTAILYNGRYYMASANVNNALYAHSL
                     IEKIRIISDAIEVYLNNKGQPLISPA"
     gene            complement(47683..48357)
                     /locus_tag="ACHOSM_RS06635"
                     /old_locus_tag="pgaptmp_001327"
     CDS             complement(47683..48357)
                     /locus_tag="ACHOSM_RS06635"
                     /old_locus_tag="pgaptmp_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGU4557887.1"
                     /translation="MKTISVESLSIISFSNIPVVTTELLASLYGTEPDYIRKNFNRNS
                     GRFVIGKHYFLLENEELREFKHSMSLRPSVKIARNVRSLILWTERGAARHAKMLETDR
                     AWEVFEKLEDCYFSQKIPEQLQLPESTLSINYPLSWFSDNHPYATMNYVDRKVISLDA
                     SVLFDMPSPTMRILNELHSKGYNVDAAVAEFNAFKHLTEEMRRKLLDISRTSEKSSRF
                     GFNVNL"
     gene            complement(48613..49386)
                     /locus_tag="ACHOSM_RS06640"
                     /old_locus_tag="pgaptmp_001328"
     CDS             complement(48613..49386)
                     /locus_tag="ACHOSM_RS06640"
                     /old_locus_tag="pgaptmp_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4557888.1"
                     /translation="MGPTSVGLVPLIASCSNPVQSTASELATSSGGYQSPIKEAAIMA
                     TIPVLSQPEITISNGRAVTTSFAVAEYFCKRHDNVLRAIANIECSEKFNALNFEDVTY
                     TDAKGEQRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKQLWEQKQLALSS
                     PAMNINFPLSWFSEHHPYATMNYVDRKVISLDASVLFDMPSPTMRILNELHSKGYNVD
                     AAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
     gene            complement(50015..50968)
                     /locus_tag="ACHOSM_RS06645"
                     /old_locus_tag="pgaptmp_001329"
     CDS             complement(50015..50968)
                     /locus_tag="ACHOSM_RS06645"
                     /old_locus_tag="pgaptmp_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HGU4557889.1"
                     /translation="MNNQNDYIEAAQQIIASLGLPRAQQNERSALCLLALLNLTPGKA
                     WADAENPLVGITPIMNWVREHYGKVYAPNTRETFRRQSMHQFCAAGVALYNPDKPDRP
                     VNSPKAVYQIEPAALSMLRTFGSPAWHDSLATYLAERETLVTRYAKEREQNRIPVEIA
                     AGQQITLSPGEHSELIRAIIEDFAPRFAPGSVLVYAGDTGEKWGYFDAPLLAGLGVDV
                     DSHGKMPDVVLHFTAKNWLLLVESVTSHGPVDGKRHAELARLFAGSTAGLVYVTAFPN
                     RSIMGRYLGEIAWETEVWVADAPSHLIHFNGVRFLGPYSTE"
     gene            complement(50965..52434)
                     /locus_tag="ACHOSM_RS06650"
                     /old_locus_tag="pgaptmp_001330"
     CDS             complement(50965..52434)
                     /locus_tag="ACHOSM_RS06650"
                     /old_locus_tag="pgaptmp_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HGU4557890.1"
                     /translation="MLQKLDVADSVRRELSPRTARKHKAEFGQFMTPSGVARFMASLF
                     PPSTMKTCRLLDAGAGLGALSCAFLDRWLVGEFGFESVEATAYEIDKKLCGHLAKHLT
                     GYRRVTPRIIEGDYIELATAEGLQDRGYTHAILNPPYKKINSQSAHRQALRTVGIETV
                     NLYSAFVALAVGEVAPGGQIVAIIPRSFCNGPYYRPFRNFILERAAIRHIHLFESRSK
                     AFRDDDVLQENIIIRLERGGRQEAVTVTTSTDDSFFDLVTYEHPFDQIVYPDDSERFI
                     HVPTTLEKSTIELSSAVQCSLADIGVKVSTGPIVDFRLKAHLRSMPEEGTVPLIYPSH
                     LSMSSTVWPVEGLKKPNAIMRNDETEKWLYPNGFYCVVRRFSSKEEKRRVVASVVDPA
                     TFSEYSVLGFENHMNVFHENKHGLPEALARGLAVFLNTTAVDKYFRRFNGHTQVNATD
                     LKMIKYPSRDTLIELGKWAMQQETLTQEHIDAKLGALTA"
     gene            complement(52529..53242)
                     /locus_tag="ACHOSM_RS06655"
                     /old_locus_tag="pgaptmp_001331"
     CDS             complement(52529..53242)
                     /locus_tag="ACHOSM_RS06655"
                     /old_locus_tag="pgaptmp_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU4557891.1"
                     /translation="MNMKKKPLTPEQLEDAKRLKSIFNAKKKELGLSQESLAYELGVT
                     QSAVNQLMAGINAINASHAAQLAKILNVKVGDFSPSLAKSIAEMALAIEEPLTRVPAY
                     EYPLLSCVQAGAFSMDDISYTAKDAIKWISTTTKASDRSFWLEVKGHSMTAPQGGKPS
                     FPEGMLILVDPEREIEDGDFCVARMNGDEFTFKRFIRESGKAYLEPLNPRFDMIECNE
                     NCQFVGKVIKSQWNDETFD"
     gene            53338..53541
                     /locus_tag="ACHOSM_RS06660"
                     /old_locus_tag="pgaptmp_001332"
     CDS             53338..53541
                     /locus_tag="ACHOSM_RS06660"
                     /old_locus_tag="pgaptmp_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU4557892.1"
                     /translation="MAMKKETLADYVYRFGQKKAAKDFGVAQSAISKALLVGREIYVK
                     TFDDGTVEAEEVRPFPAFVRGDD"
     gene            53596..53865
                     /locus_tag="ACHOSM_RS06665"
                     /old_locus_tag="pgaptmp_001333"
     CDS             53596..53865
                     /locus_tag="ACHOSM_RS06665"
                     /old_locus_tag="pgaptmp_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557893.1"
                     /translation="MPEQYCDADRDWIQEQLLQLDPTTRVKIAAEYARVYQEEWDKEP
                     VSFRKGNRARRSANTRLRVYVQKYARASRGWMLSPVAVRKFPPTP"
     gene            54073..54450
                     /locus_tag="ACHOSM_RS06670"
                     /old_locus_tag="pgaptmp_001334"
     CDS             54073..54450
                     /locus_tag="ACHOSM_RS06670"
                     /old_locus_tag="pgaptmp_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557894.1"
                     /translation="MSAATKSALIRTLSTVPLRTEERYSFLADVVTILESQGMHVASN
                     VTVRIDGRNFRVDILATAKTGGSVAIEIDRSSPRPRSVMKLRELARRGTEGFVLLRMP
                     KKLTSYSDAGIDIIPANGKGASC"
     gene            54444..55964
                     /locus_tag="ACHOSM_RS06675"
                     /old_locus_tag="pgaptmp_001335"
     CDS             54444..55964
                     /locus_tag="ACHOSM_RS06675"
                     /old_locus_tag="pgaptmp_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGU4557895.1"
                     /translation="MLKITPNFAQERGLNQLRHQWKQHRTYLMYAPTGSGKTGLAAFV
                     TAGMVERGMRVMFVCPYTILLNQTAERFTEYGLPWEEISFVWRDHPNYDPSRLIQIAS
                     ADTLIRREFPDNIDLLIIDEAHMKRRALLEVIRDKDIRVLGLSGTPFAAWMGKYYECL
                     IKPTTIRELIQRGDLSDYEFFAPSMPDLAGVKTSNTVFGRDYNEEQLASIMGSSDLVG
                     DIVSNWLENGEDLPTICFCVNVAHANFVTREFLQSGIGAEVMTADTPHDERQDIIRRF
                     EEGATKIIVNVGVLVAGFDSDVRCLIYARPTKSEIRWLQCIGRALRTASGKKRALIFD
                     HSGTVHRLGFPEDIEYDELPGKNDGMKASAGGSEVKAEKLPRECPKCHFMKPAGVHMC
                     PKCGFRPLGGDDVATDRDRKLSRVNKGKREYTREEKQRWWSEIKGYQNYRNATGKPLS
                     DGWCAHTYKEKFGVWPKGFSNAPLQTSVEVYNFIKSKTIAYAKGRKKAMTGGQHAN"
     gene            55954..56925
                     /locus_tag="ACHOSM_RS06680"
                     /old_locus_tag="pgaptmp_001336"
     CDS             55954..56925
                     /locus_tag="ACHOSM_RS06680"
                     /old_locus_tag="pgaptmp_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HGU4557896.1"
                     /translation="MRTRDAAVGHWSRIFEYYGMPPVTGVKHYNGPCPICGARGKFRC
                     DDKDGSGSWICVCGHGDGMNLLQLATGKPWVTLCDEIDRLIGNTWKREKVSQPVTEIS
                     RKRELVIDKFAGLPCLRGTTGEAYLQGRGILQLPTESVRFCDRQIASGREYQAIYAIA
                     TDDKGSLCYLHRTLLDGDRKANVEAAKKMTALQELPGLQHAKSVAIRLYPVSSTLGIA
                     EGIETALSCRQIFRCNVWSTMNSGFMEKFIAPPGVNHLIIFADNDAHGAGLAAAFKCG
                     HKNLMSCNDVEKVSIRWPDLPDFNDMLIQGCEAREHVLTRKFKAEAA"
     gene            56925..57374
                     /locus_tag="ACHOSM_RS06685"
                     /old_locus_tag="pgaptmp_001337"
     CDS             56925..57374
                     /locus_tag="ACHOSM_RS06685"
                     /old_locus_tag="pgaptmp_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HGU4557897.1"
                     /translation="MEIEMIKAANGVFVPAYERDLPRLAKFKNGELYTLEAKLTRNPS
                     FHRKMFAFLNFCFQYWCAEHAGYEFSDEATQFDEFRKNLTILAGFYDVVTTIRGEVRY
                     RAKSLSYANMDQDEFERCYNAMINAALKHVFGRSNNPELNNRLLSFF"
     gene            57382..57945
                     /locus_tag="ACHOSM_RS06690"
                     /old_locus_tag="pgaptmp_001338"
     CDS             57382..57945
                     /locus_tag="ACHOSM_RS06690"
                     /old_locus_tag="pgaptmp_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU4557898.1"
                     /translation="MKQRKPKKCKVCGSSFVPFRSYQKVCCGQCALELVRKEKAIASA
                     KEQADKLKARRRDLQPRSYWIKQAQQAVNAYIRERDRHLPCVSCGTFDSAQWDAGHYR
                     TTAAAPQLRFDERNIHKQCVVCNQYKSGNLVPYRVELINRIGQEAVDEIESNHSRHRW
                     TVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            57942..58136
                     /locus_tag="ACHOSM_RS06695"
                     /old_locus_tag="pgaptmp_001339"
     CDS             57942..58136
                     /locus_tag="ACHOSM_RS06695"
                     /old_locus_tag="pgaptmp_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HGU4557899.1"
                     /translation="MTFTVKTIPDMLVEAYGNQTEVARILNCNRATVRKYIGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            58129..>58226
                     /locus_tag="ACHOSM_RS06700"
                     /old_locus_tag="pgaptmp_001340"
     CDS             58129..>58226
                     /locus_tag="ACHOSM_RS06700"
                     /old_locus_tag="pgaptmp_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4557900.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFK"
BASE COUNT        14000 a        14308 c        14880 g        15038 t
ORIGIN      
        1 ttcaggcctg gagcagtcca tgttttacaa aaatactcgg cagcgtaacg tgttgcaaga
       61 gcggcatcag acgaggaacc tgaggttccg ctcatgaccg ataatctcat ccgaataaaa
      121 atataccaat tggtatacca agtttatacc aagagccaaa aaagcaaagg ggctaccatt
      181 tggtaacccc ttgattattt ggcggaagcg cagagattcg aactctggaa ccctttcggg
      241 tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga atgacgcgca
      301 ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct gaaaaacagc
      361 caaaagtgca ttgatagcgt gaaataacag cagattgatc atttcatcac catgaattcc
      421 ttctctttta ctcgtttagc aaccggctaa acatccccac cgcccggcca aaagaaaaat
      481 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
      541 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
      601 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
      661 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
      721 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
      781 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
      841 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgca
      901 cctgctgtac cgaatctttt atccgctccg ctcacccgct ggcaaaggac gtcatccttt
      961 cccttatttc cctcgattac gacgatactt tgatggctgc cgccggaacc ctggcggaag
     1021 aagtcttcga agacatcatc acgcaataca atggcaaata tatcctcgca gtagaaggta
     1081 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
     1141 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
     1201 ggggctgcgt gcaggccgcg cggcccaatc cgacgcaggc aacgcctatc gacaaagtca
     1261 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
     1321 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggccgtc
     1381 cgctgatgtt ctatggtcag cgaatccacg ataaatgcta tcgccgcgcc cacttcgacg
     1441 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttac tgcctgtaca
     1501 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
     1561 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
     1621 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
     1681 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcggctgtt ggtgtgcacg
     1741 cagtcgccag cgccgttgac cagcgcagac gtcataacca gcaacctaca gaaaccgaac
     1801 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
     1861 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
     1921 gcgctgcgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
     1981 catgtttcgc gggctggaga tcattctgca agggcgcgac ccgcgcgatg cgtgggcgtt
     2041 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
     2101 cgaagatgct atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
     2161 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
     2221 gatcgatgtg ttagatgcgc tgaaagccga cccgcggaaa acctccgaac tggcgcaaag
     2281 tctctcctca tggccgaaat catcccctgg ctatttcttc gacgtacaaa accgcctgaa
     2341 aaaatttgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
     2401 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctag aagctctcga
     2461 tttccagcgt gaaattgtca aaatccacgc ggtctttggc ggtaaaaacc cgcatccgaa
     2521 ctggattgtt ggcgggatgc cgtgcgccat taacattgat gaaagcggcg cggtcggggc
     2581 agtcaatatg gaacgcctga acctggtgca gtcaattatc acccgcacgg cggacttcat
     2641 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
     2701 aatcggcact ggtctttctg ataaatgcgt tctcagctac ggcgcattcc cggatattgc
     2761 caacgacttt ggcgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
     2821 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgacca
     2881 cgcctggtat cgatatccca acgatcaggt cgggcgtcat ccgttcgatg gcatcaccga
     2941 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
     3001 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
     3061 gccgctggcg cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
     3121 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttaggccg
     3181 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
     3241 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccacgg aaaaatggga
     3301 acctgcaacc tggccgacag agtgccgtgg tgtcggtttt accgaagcgc cgcgtggggc
     3361 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
     3421 gaccacctgg aacgccagcc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
     3481 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatcctgc gtactctgca
     3541 cagctttgac ccgtgcctcg cctgttcaac acacgtgctg ggcgacgacg gtagcgagct
     3601 gatctccgtg caggtgcgtt aacagcgaag gaggatcatc atgcaacaga aaagcgacaa
     3661 cgttgtcagc cactatgtct ttgaagcgcc agtccgcatc tggcactggt tgacggtgtt
     3721 atgcatggcg gtgttgatgg tcaccggata ctttatcggc aagccgctac cttccgtcag
     3781 cggcgaggcg acgtatctgt tctatatggg ctacatcagg ttaattcact tcagcgccgg
     3841 gatggttttt accgtggttt tgctgatgcg gatctactgg gcttttgttg gcaatcgata
     3901 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
     3961 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataatcc
     4021 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcactgg
     4081 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
     4141 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
     4201 gatgtggctg attggcgcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
     4261 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
     4321 caaaataagt aacaaggagc gttcatgagc gagcaacgcg tggtggtcat ggggctgggc
     4381 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
     4441 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
     4501 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
     4561 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cttatctcag cgcgaagaaa
     4621 atgagcctgc atcagaacag tttctccgaa gtgttggcgc tggcggatat ccgcggacat
     4681 ctgccagcac atattgccct cgtcggtctg caacccgcaa tgctcgacga ctacggcggt
     4741 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaggcggc gctggcgcag
     4801 ctggctgcgt ggggaattgt gccgcaaccg gctaatgaat cgcgctgtct caattatgac
     4861 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
     4921 gagcagggat aagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
     4981 gctggacgcc agtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
     5041 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
     5101 ttggcgaatt actgcgcgag tttcccgact atacatggca ggtggcgatt gctgaccttg
     5161 agcagagcga agccatcggc gatcgctttg gcgtctttcg ctttcctgcc actttagtgt
     5221 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
     5281 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
     5341 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
     5401 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ctggagcaat gtgctcacag
     5461 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccaaccgcc
     5521 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
     5581 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtga
     5641 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
     5701 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
     5761 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
     5821 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
     5881 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcacgtct
     5941 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
     6001 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
     6061 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
     6121 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
     6181 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
     6241 cgtagagttt caggataaag agggagatct accattctcg ggttattttt ctctcttcgc
     6301 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
     6361 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
     6421 tggaaaccat ttacgtggtc accggcaaaa cgatctaccg cgatatgacg cgcttctggg
     6481 gtaagctctt cggtatcaac tttgctcttg gcgtggctac cggcctgacc atggagtttc
     6541 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccgc
     6601 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg cttttcttct
     6661 tcggctggca gcggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
     6721 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
     6781 gtgcgcattt tgatatcgac accctacgta tggagatgac cagcttcagc gagctggtct
     6841 ttaacccggt cagccaggtg aaatttgtgc acaccgtaat ggcgggctac gtgaccgggg
     6901 ccatgtttat tatggcgatc agcgcttggt atttactgcg cggacgggag cgcgatgttg
     6961 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggcacgctac
     7021 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
     7081 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
     7141 aacaggatca agagcgtaac gcctttgccc tcaaaattcc cgcgctgtta gggatcctcg
     7201 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
     7261 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatcg caaggcaatc
     7321 gtgagccgca tgtgttgcag gcattccggg aactggaagg tgacctgggc tacggcatgt
     7381 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
     7441 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
     7501 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
     7561 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
     7621 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
     7681 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
     7741 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
     7801 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
     7861 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
     7921 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
     7981 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagtgttg gtgcgcactg
     8041 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgccg cctggcccag
     8101 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
     8161 gttcttccgc ccgctggcct ttgattatcg cggaaaaatc gccgatgcac gctggcgtaa
     8221 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
     8281 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
     8341 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgcggat tgctcagcct
     8401 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
     8461 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
     8521 ggcgggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
     8581 cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
     8641 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttctttt gcccattgct
     8701 gacggtgatg gcgatttatc gtggtcgccc ggggtgggga tttttgatgg cgtcattgat
     8761 gcaatttggc gtgattttca cggcaggcat cacgctgttc cccttcgtca tgccgtcaag
     8821 cgtgagtccg atctccagcc tgacgttgtg ggacagcact tccagccagc tgacgctgag
     8881 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
     8941 ctactacaaa atgtgggggc gcatgacaac agaaactctc cgccgtaacg aaaacgagtt
     9001 gtactaagga gcaggaacaa tgtggtattt actttggttc gtcggcattt tgttgatgtg
     9061 ttcgctctcc acccttgtgt tggtatggct ggacccgcgt ctgaaaagtt aacgaacgta
     9121 ggcctgatgc ggcgcattag catcgcatca ggcaatcaat aatgtcagat atgaaaagcg
     9181 gaaacatatc gatgaaagcg atcttaatcc catttttatc tcttctgatt ccgttaacct
     9241 cgcaatctgc attcgctcag agtgagccgg agctgaagct ggaaagtgtg gtgattgtca
     9301 gtcgtcatgg tgtgcgtgct ccaaccaagg ccacgcaact gatgcaggat gtcaccccag
     9361 acgcatggcc aacctggccg gtaaaactgg gttggctgac accgcgcggt ggtgagctaa
     9421 tcgcctatct cggacattac caacgccagc gtctggtagc cgacggattg ctggcgaaaa
     9481 agggctgccc gcagtctggt caggtcgcga ttattgctga tgtcgacgag cgtacccgta
     9541 aaacaggcga agccttcgcc gccgggctgg cacctgactg tacaataacc gtacataccc
     9601 aggcagatac gtccagtccc gatctgttat ttaatcctct aaaaactggc gtttgccaac
     9661 tggataacgc gaacgtgact gacgcgatcc tcagcagggc aggagggtca attgctgact
     9721 ttaccgggca tcggcaaacg gcgtttcgcg aactggaacg ggtgcttaat tttccgcaat
     9781 caaacttgtg ccttaaacgt gagaaacagg acgaaagctg ttcattaacg caggcattac
     9841 catcggaact caaggtgagc gccgacaatg tctcattaac cgatgcggta agcctcgcat
     9901 caatgctgac ggagatattt ctcctgcaac aagcacaggg aatgccggag ccggggtggg
     9961 gaaggatcac tgattcacac cagtggaaca ccttgctaag tttgcataac gcgcaatttt
    10021 atttgctaca acgcacgcca gaggttgccc gcagccgcgc caccccgtta ttagatttga
    10081 tcaagacagc gttgacgccc catccaccgc aaaaacaggc gtatggtgtg acattaccca
    10141 cttcagtgct gtttatcgcc ggacacgata ctaatctggc aaatctcggc ggcgcactgg
    10201 agctcaactg gacgcttccc ggtcagccgg ataacacgcc gccaggtggt gaactggtgt
    10261 ttgaacgctg gcgtcggcta agcgataaca gccagtggat tcaggtttcg ctggtcttcc
    10321 agactttaca gcagatgcgt gataaaacgc cgctgtcatt aaatacgccg cccggagagg
    10381 tgaaactgac cctggcagga tgtgaagagc gaaatgcgca gggcatgtgt tcgttggcag
    10441 gttttacgca aatcgtgaat gaagcacgca taccggcgtg cagtttgtaa tgcataaaaa
    10501 agagcattca gttacctgaa tgctctgagg ctgatgacaa acgaagaact gtctaatgcg
    10561 tagaccggaa aaggcgttca cgccgcatcc ggccactttc agttttactc tttctcggag
    10621 taactataac cgtaatagtt atagccgtaa ctgtaagcgg tgctggcgcg tttaatcaca
    10681 ccattgagga tagcgccttt aatattgacg cctgcctgtt ccagacgctg cattgacaaa
    10741 ctcacctctt tggcggtgtt caagccaaaa cgcgcaacca gcaggctggt gccaacagaa
    10801 cgccccacga ccgcggcatc actcaccgcc agcatcggcg gcgtatcgac aatcaccaga
    10861 tcgtaatggt cgttcgccca ttccagtaat tgacgcatcc gatcgcgcat cagcagttca
    10921 gacgggttag gtggcacctg accgcgagta atcacatcaa agcctccttt gccaaaatgc
    10981 tggatcactt tgttgagctc atctttacct gccagatatt ccgacaagcc atgttcatta
    11041 ctcacggtaa acaggttatg cgaataacca cggcgtaagt cggcatcaat aaataacact
    11101 ttttgatcgg actgggcgat caccgctgcc agagttgaac tgacaaacgt tttaccactg
    11161 tctggcgtcg caccggtgat catcagaatg ttattctccg tctccatcat agcgaagtgc
    11221 agactggttc gtagcgcacg tacggcttcc acagcagaat ccgccgggtt atccaccgcc
    11281 aggaagggga tatttttagt acgatggcgc tgctgattag aaaataaatt tttcttacgc
    11341 agacgggtac gtttatccag ccactcggac attgggatag tggcataaac gctgatgccg
    11401 tgctcttcca gttgttccgg ggcttctaca ccacgacgca acatcgcacg cgccagcacg
    11461 gcacccactg aaataaacag gccaagaata aaaccaagca ccacgttcaa cgcttttttc
    11521 ggtttcactg gctgcggctg agtgactgcc gggtcgataa tccgcacgtt accaatggca
    11581 ctggatttcg aaatactcaa ctcctgctgg cggttaagta attgcagata taccgcacgg
    11641 cccgcttcta cgtcacggct taaacgtaac acttcctgtt gagtggaagg cattgccgat
    11701 acccgcttat tcaggcgttt gcgttcttgc tccagcgtct ggcgtttttc cagcagcgca
    11761 cgataagttg ggtgatcttt cttatacagc tgggagatct ctgcctcgcg gaaggtcagc
    11821 tcattgagtt gattatcaac gttcacaatc tgctcaagaa cggctttggc ttccaggtta
    11881 aggtcaaccg aatcgcgctg ctggcgataa acgttgagtt tttcttccgc ctggtccagc
    11941 tcgctgcgca cttcaggtaa ctggcgctgt aagaattcaa ggctttgtga atcctgcgcc
    12001 gcctggcgag cgatattctg ttgcaaatag ttgttagcga tgctgttcag aatacgagta
    12061 atcaactggg gatcatcacc agtcatggta agttccagca tcccgctttc tttactgcgt
    12121 tcgctaacgg taaaggtttc ctgcaatgcg ttaatcgctt ccagttcggt acgctggctc
    12181 aggacaaact gtgttcctgg tttggcctta atgtccgcga tagtcagcgc aacgccatct
    12241 ttttccagac gctggccgac cataccattg acggtgaact cttcaccttc cagtgtatag
    12301 tggccgtttt ccccaaccgt gagtgtcagt tgctgatcct gaccattcag ttgtggaata
    12361 tgcatccagc tgatcgccag ctcacctggt ttttctttgg ttaatctcgc ccagccgcga
    12421 cccacaatcg gaaaatactt ctgctcaact atgtcgcgca gattcagttc agcaatggtt
    12481 ttaccgagaa tcatgcgcga ttgcagcagt tggatctccg gtgcagactc gggcgatgag
    12541 ttagggatca tatcgctcag gccgctgaga atggcgttgc cctgtttttg ctcaacctgg
    12601 accagagtat ctgcctgata aattggtgtg cttaacagcg agtaagcgac agcgatcagc
    12661 gtgaataacg cggtcacgct gataataaac ttacggtgat cccataactc gccgaccaga
    12721 cgaagcagat cgatctcatt ttcctgagtg ctgcctggtg gcgtattcat atttttagtt
    12781 gtcatcgtat tttataaact caaattaccg gctgaggcgc ttcgcccatt cctgactggc
    12841 gcgctccaac ataccgtaga catgttcaaa tgcgtcctga cttttacgat agggatccgg
    12901 gatctctttc tgttccagcc attgcccaaa cagcattgtc ttcccgcgga cctcgggagc
    12961 aattgcggta acctgagcaa tatgttccga ctccattgcc agaatcagat cgtaatttcg
    13021 tgccatctca gcagtgagct tacgtccggc atgtccttca agagatacgc catgattagc
    13081 ggcaacatct gccgctgtcg catctgcagg gtgttttacc agaccatgaa cacctgccga
    13141 tttgactttt acgcccggca gacgtttacg cagcaagcgc tcgccaatgg gcgaacggca
    13201 aatgttgccg gtacaaacca ccaggattga gttaaatttt agttgggcca tgtttgaatc
    13261 caccgtgaag tctctgtcag gtcatgcacc ccggaaatcg tcggtaccag cagagaaatc
    13321 acacggttcc aacgagccag cggcgcggta gtgacataaa caatgtcgta aggttcaagc
    13381 tggaattccg ttccgagtat cattgccgat gcgtccttcg cattcagctg ataaatattg
    13441 gcgattttgc cattctgctt gttttgcgtt gcacgaataa caaaaatccc cgtcgcatca
    13501 gcgacatcct gattcatacc ctcagcgtta cccagcgctt ccgccagggt cataccgctg
    13561 cggtccattt tcagcgtgct ctgtttaccc acttcaccca tcacgaagac tttcagatca
    13621 tcgttgcgcg gaataaataa aatatcgccc gggtgcagca gtttgttctg gcgcaagtcg
    13681 ccacgctgca tcagcgcata gaggttcact ttggtcttta cgccattctg ggtgagcacc
    13741 acgttgcgcc agtctgcatc tgtggtcaga ccgcccgcgg cgttaatcgc atccatgatg
    13801 gtcagaggaa tattggtaat cggctgctga cccgatttgc tgacttcgcc ggtgacgtac
    13861 gctttttgcg atcggaacgc cgcgacgctg acatcgactt gcgggctttc aatgactgaa
    13921 tcgagacgcg ctgtaatctc gttgcgtacc tgggtaagtg ttttcccggc cactttcagg
    13981 cgaccaatat aggggtagaa aatcgcacca tcggcgttaa cccagttgcc ggtatcactg
    14041 gcgctacggt attgccctgc gggggtggtt agttccgggt gatcccagac cgtaaccatc
    14101 aacacatcac caatcccgat acgatattca tagtttgcga tttgctgctc cagctccggg
    14161 ttagcctgcg acataatcgg tttggcgcgc aactgatcaa tcaatcctgg cgtgacggga
    14221 tagatattga ccattttgtc caggtcatac tgattgtcag gtagttcaat gacatccttg
    14281 ttactggtcg ataaattctg accaggaaca agggtacatg ctgtcaaaga cagaacagcc
    14341 aatgtcagta ccgaaaactt aaaaatgttc tttttcattg gtttatattt atcagtcaat
    14401 gtaatgttat gaattacgtc aggttattgt ccaaccggta cttctcgctc tgccgtcatt
    14461 ggatagagct ggtatttgcg tgacagcatt tgtccaccat cacgcgtcaa tggtgtccac
    14521 gaaaccaccg cgcggttgcg gttaggtcca atggtcatca agtcgaatgg aatcgagata
    14581 taaaaacctt tactaaagcc gccttcgccg tagtcatctt tagatacgtt actaattgcc
    14641 gcccataccc ctaccgccac gccgctgtca aagcgtttgg cgatgtcgat cgttgcccct
    14701 ttatcttttg ccagatattg cccaacgcta agtttcatca gtacgccatt gagcgtcggc
    14761 gggttccagt aagccgtcac gaaaccagtt ggcgtggaat aatcggtgaa gcgcatcatg
    14821 ttgtcccagt cacgttgctt cacgtagtta acgtccacac ccagcgccca gctggcatct
    14881 agcgggcgat aaagcagctc ggaaccgaca ccggcgtaca tcgtttccag ataaccgcca
    14941 tacacctggc catagaaacc attgcctaag tcggcaaagt agttcgcctg caagttgttg
    15001 agataaacgt cattgcgaac gtaatcacgg atatgcgtgc gcacgcgggg cagggtagag
    15061 tccgcgggca acagcgaaga cttaaacttg tcgtagttgt tgtaaatatt ggtgaaaata
    15121 ccgccatcaa gcagcaggtg gtcggtaaac cagtagcggg cactggacat cagccccagc
    15181 tggaacatat agaaatcttc cgggccgccc agcgactgtg aaagtgttgg gttgaaagag
    15241 tagctaaagc gatcttcacg aatacggtag ccccgaccaa aggcagaaag atcttctgct
    15301 tcaacacgtt gttgttgaag ttgctctgat tgaccaggcg ctgtaccagc cagctgtttg
    15361 cgcaggctgg ctacgtcggt ttcggtagtc accatcgcca tatgctcgcg cttttgcgtc
    15421 acgctaatct tctcaacgcc ttgcggcagg ttattcacca gaatccggtt ggcacgatcg
    15481 accgcttcgc gagaatcacg gtatttgtat tgttgaccag acatatacag cgtcttatcg
    15541 cgcagctgaa tttccggtgc ttcaaaacct gcgttgtact tcagcgcggt aagttggttc
    15601 gctactgtgg tgtactgcaa tccttcagat tcaggcgcag gttgatatgc cggttttggc
    15661 gtatcgcgca gggcagggcg cagatcgttg aaattggtcc gtaacgtgaa gccaaacatc
    15721 aacgtgttac cgcgttcata actcaggttg agatctgccc agctggcagc gcgataaact
    15781 gcgccgacgt tgaaatggct tgcctgaggc agtttgccag cgaaatcatt ctggtaattg
    15841 ttgccatcgt attcgagttt cagacgcagg ggattccacg gcgtttgata ctcaatgccg
    15901 ccaaagatgg aagccgggcc acgaaagata tcgctaaagc tgatatcgcc cgcatcgtga
    15961 gactctgcgc gatgacaata tttatcgctc acccggcaaa acgggttggt aatattgccc
    16021 gcattaccgg cgtagcccca tgccatcccg agggtgaaat caaatggccc cgcttgcttg
    16081 ctggccacca gatactcacc gtcaaacaga cccgtaccag caatatcacg tttaccaaac
    16141 gccacttgcg gtagccagta accttcttcc cacaggcgaa gtttaaaatc gaatgatttg
    16201 tctttgtatg actgatcgcc gctgaaatct tcccactggc tatatttgcg tgtgcgcaca
    16261 tccgtataac gaatggtgcc ttccagccat gggaacagcg ccacggaggt ggagtagaac
    16321 cggtattgat cgttatcccg gtagttgacg ctgaattcac cttccggtgc gatgcgcgca
    16381 tttggcatct gcaacaatcc tgtgccgcca aagtctgatt gcgacgaacc cagcggatcc
    16441 gggtaggtta atacttcagc atgacaggca ctggagacgg caatggccag gcagcttaaa
    16501 agataagaat tcttcttcat cagtcaggaa cacgttgcgt taaaacagac acaatctggt
    16561 catttaaatc agcatatttt tcgggcagta catgcgcaga aaagcccaac caaagctggc
    16621 tgccaggcgg tggttccaca tgtcttttat tccacagggc aacaggggcg acgacagtct
    16681 caccctcagg cgtaatcacc ataacgttat tcttgtccgc gcctgcaagg cgggggtgat
    16741 cttgcagata atcggtaacg ctacggcctg cctgccaggg caattgccca gccccagaaa
    16801 ccgcacccag cagagtaata gtcaccggac gctgtacggt atagagcgta tagtccccga
    16861 ccaacggggg attgctgttc tcatccactc gtacaaaatc agggtccagt ttgaccggta
    16921 agcgaccagt gatattgaga ttgagtaact gctggcgcac ggatttaatc gttgccgcca
    16981 catcgtcgtc agcttctgct tcccaggaag caagctgtgc catgacatgt tggtagtctt
    17041 taagcgcttt agctttcgcc gcagagtcgg tcagcaaggc accaggccac cagaggcgat
    17101 cgcgcagttg cggttgtgtc accaactgga caacattttc cacggggcca acggaaagtg
    17161 tctgttgttc gccaggcaga taaatagtca ccgttccttg cgcaaaggca tggggtgtca
    17221 ttacgtaaag tacgctggca atgaaatacg actgtaattt attcatggtg cgggtttaag
    17281 aaacgtcatt tctacgggaa taacccctgc gcctaacatt tgccgacttt gacgaacctg
    17341 gccagtagcc gaatcgaccc agaatgtgtt ttgccactgt ttttctggcc gcgtggaagt
    17401 cacctcttcc tgccagacat tacacagcac attgcgaccc gcaatattca gcgtctctaa
    17461 tccagcaaag gaaaaagtag aactcagggt cgcagagcga aaatggttgt cttcactcca
    17521 gagaatatcg cgcgtccagc ggctgccatc ttttattgcc agcgcgttac gcagagggtc
    17581 ctgcccggaa tttgtcactt ccagcagatt attattaagt ttgacggttt ttaaaagacg
    17641 tccattgtgg gtaaccagca tggcgttatc ctgggataac catttgcttt gttcttgttc
    17701 tatatatccc agtaccacaa agattcgttg cccttcatta aggcgtaaat acatcgtgga
    17761 ataaggcaac gcctggatct gctgatccgc tacggtgata tcctgattat cgaaaaggct
    17821 ggcgcgaaat gtatcgacca tactttgctg gctgtgcgta catcccgcaa gaaatagtgc
    17881 gaaaatcgat aaaataagag ggcgcacctt taatccttga aaggttaatt aaaaaaatgc
    17941 aaatctctaa agcccggtac gaatatcgca accgggtatc atttaacttt aatttcgacg
    18001 cgttaaagca gtattagcgt gtagtggacg tggtggttga ggtgttatga gaaccgccat
    18061 cgccactgtc atttgctgca acaactgcca acgtcgccac accaacagcc gttgccacac
    18121 cgacaactgt cgccgtgccg acagcgccag cagccacgcc accagtggta gtagcagtcg
    18181 tggttgcgcc ggtagtggtc gttgtggtgc cagtagtacc cgtcgttgcc tcggtcccgt
    18241 ttgttgcttc aggggcagca aacgcagctg gcgtcgtcaa catgaaggcc ataaggattg
    18301 cagaaagctt gtgtttcata acttttcctt tattcatcgc atggacaata cgtacgcttg
    18361 agatcaggat tgtctacttt gagtataaca tccttgtgaa atctcccttt cctcataccg
    18421 cttgcactat atactgcggg tagaaaaggc gcgcagaggg aaatataaga ttgttaacta
    18481 tccccctctg gaaatacgac ttgtgagcat aagataaatc ttagcaaaat acaaattaaa
    18541 gataaggaaa ttgttacgaa agctattaat tattgttggt aatatagttt caagtggtac
    18601 tataactggc tgttaaatgg catttataac tattaggtgc agagatattc gcttaaatgg
    18661 attagtttct ttgtggaaaa gtaactgatg ttataatgta tgatgttgac tattgcacta
    18721 ttccaataaa accagtcagc tttaaacaag cagcgtcata ttaagagaga taaacatttg
    18781 ccgctgttgg tcctcgcagg ccatttacgc ggcaaaattc cacacgtaat cctggtataa
    18841 gcacttctgc gtcgcgggga gtgaatgcgg aaatatggac ctgaacttct ttacgaccgt
    18901 cggaggggat aatgaatcct ttgccgcttt tgcgatcaaa ggttttgaca attcctgtca
    18961 ttttacggga caaacaaatt ccttactgaa aatactgcgc tgcactatac ggggttaata
    19021 aaataaagcc agcgatattt aagaccgccg gacggctaaa ataaaatttg cttaatctca
    19081 attatcatgc gttaatagct gcgtcggttt gaaagacaga cagcatacaa agtagtttac
    19141 taaagcagtt ctcattatca ggcattatcc ccttcttttg agtctctctc ctgaacacta
    19201 agtagtttct gtattaaagc cctgtttgcc gaaaggccca aaatgaagga agtaaaatat
    19261 gtctaataaa atgactggtt tagtaaaatg gtttaacgca gataaaggtt ttggctttat
    19321 cactcctgat gatggcagca aagacgtttt cgtccatttc accgccatcc agagcaatga
    19381 attccgcacg ctgaacgaaa atcagaaagt tgaattttct attgagcagg ggcaacgtgg
    19441 ccccgcggca gcgaacgttg ttacgctcta aggttgccgt tattactcaa catctccatt
    19501 tccgctgtcc atgttgtcat ggttcacagt accgcacatc ggcattcgat gtgacggagc
    19561 gaaacccttt gggcgctaag tgtatttttt gtaaatcgac gatgatcacc tttgataacg
    19621 tcgcgctgca aatacgcact gaccatgtgc cgctggattt cacaaaataa tatcaggctc
    19681 cctcgtggag ccttttttat atctgcctta tttttcttca acgctgtatg tatagtaagc
    19741 gataacctgt tgattattga atctttcggg gagatggctt ataacatttc ttacctgacc
    19801 agggtaccgg gaaccaacac cttactggcg tgttgctgtc ttttaagacc agaagaggtt
    19861 aacagtgaat attgaagagt taaaaaaaca agccgaaacg gaaatcgccg actttatcgc
    19921 gcaaaaaatc gccgaactga acaagaatac agggaaagaa gtctctgaaa ttcgcttcac
    19981 cgcacgagaa aaaatgaccg ggcttgaaag ttatgatgtc aaaatcaaaa taatgtgatt
    20041 ttgtgaacat caccccgtgc gaggtgatgt tccgcttgtt gctaatttag tgaccaatca
    20101 ttggcgcttg tggaattaag cgtcggtaca attcctccgg caccgggctt tgccatactc
    20161 ccgcatacat tgcgtaacca atcaccgcaa acataatccc cagaaccagt agcgtcatta
    20221 accagccaga caacgcaaaa gcttttttat ttgccgcagg tttttgcagt gaaaaggtca
    20281 atgttgaggc taccggacat gactctacgc aagtcataca gccggtacat tccactgttc
    20341 gtacctgaat taatttatct accgggatcc gtgatgggca attttttgcg catttgccac
    20401 agccgataca actttcggca ttgcgacgaa tcttaaacgg cgataatagc gaaaccacgc
    20461 ccatcagcgc gccatatggg caaagataac gacaccaggc atggcgaata aacaggctgg
    20521 caatcagcaa aacggtcacg ctgattaatg tcgcggtccc catatagcga aagaaatcga
    20581 gcattttaac gtccatcacc acgctgtagg gcgacaacat aaaatagtga atcgcctgag
    20641 cgggcatcaa taacgcgata tagagaaaaa aactcaacag caaatacttt acaccgcgca
    20701 gaggaatatc cagccagcgg ggaaggacaa attgccgacc aaacagtttg ttaccgagat
    20761 cgccgattaa ttcagaaagc gtaccaaccg ggcataacca tgagcaaaag gcctttttga
    20821 gtaatagact gatgacgata aaagcgacca ataacagcat cgcggcggcg tggacggacg
    20881 gtaactgacc tgttgcaagg ctatatttca gattcatcag accggcaatc ggtagccagc
    20941 cttcgatacc tcccggtctg gcgacaaatg tcgtgctact tgccgtttcg taatagcgca
    21001 cccaatacca gaacgtgatg gcaatataaa tattcattgc caacagtaat aattgcgtcg
    21061 ctttacgcca ggtcgtggca ttgcgccagt cattccacgg taatttgccg ccgtcgtgcc
    21121 tggccgccgc tgccagcggg ttcttttttt ctctgccatg attttgccaa tccgttaagt
    21181 tgcataccaa atgccactat tctagttatt cttaactggc tgatattgat tcaaatcgcg
    21241 ttcaggtctt tcttatgcaa ccatgcttcc agagcggcaa cactgcgtgt aatttcttcg
    21301 tgtggaaggg gggcagataa tggctgctgc tccagttgtg cgcatagctg gctggcgata
    21361 cgcattccca aactcgagca actgcttttt agctgatgcg cggcacgctt tattttctcg
    21421 ctatcctgac tggcgcgggc aatgtcgatt tcatcgagaa gcggcagggc atgttgtgta
    21481 aataatacca gccattcgtg gatcttctcc gtccccatta actgagcatc ttcattgagt
    21541 tgcgatacat ccagcgattg atcgttattg acttgcagtt ggagatagtg cgccagtaac
    21601 tgaccgagca cttcacgcgg caccggttta gggataatcc cgcggaataa tgaactggta
    21661 cgctggcgca gcgtttcgtc aatgacatgg gcgctaaagc caatcaaaac cagcgacgga
    21721 tattgctgtg ccagttgtcg ggcaagcgta atgccgtcga catccggcag atcaaaatcc
    21781 accagtgagg cagcaaacgg ttcgctattt tgcagtgtct ctaaagcctg cgcggcattg
    21841 ccaacagcaa caacctgcgc accactggtg ttcagcatct caacggtaat tcgctgggtt
    21901 agcgggttat cttcaattaa cagcaaacgt aaaccgtcaa gacgcaccgc ctgattgact
    21961 gtttttggca cgggtgccgt ggcaacacgt aacggcaagc gtaaacaaaa acagcttcca
    22021 acctccggcg tgctggaggc gctcagttcg ccgcccatcg cctgggccag acggctactg
    22081 atagtcagtc ccagcccggt gccgccgcgt ttgccgctta cctgcacaaa tggctggaag
    22141 atttctgcca gtttcgcggg atcaataccg cagccgctgt cttccacttc gaccagccat
    22201 tgctcgccat cagtgcgact acgcaggata atgtaccctt cgtcagtaaa acgcagggcg
    22261 ttgctcaaca ggttggttat aacctgacga atacgtcgtg gatcgcccat taacgcgcac
    22321 ggcatatcat cggcaattgc cgttgccagg cgaatcgggc gacctttcac ccgtccgctc
    22381 attaattgca gggtactttc cagcagcggg cgcggttcaa agggctcatc gctgaccgaa
    22441 acattcttgc cacctgcttc gatagcggaa taatcgagaa tatcgttgag gatggtcagc
    22501 aacgattcac cagagtcagt aattgcccgc aaatcatcac gctgggcgtt aagtgcgggg
    22561 ttatctgcca gcagttgagc agtgccgaga ataccgtaca gcggtgtgcg gatctcatgg
    22621 ctcatcgccg ccagaaacgc cgattttgcc tggctggctt tttctgcttc cgcccgtgcc
    22681 tgtcggtgtt ctatcaccag ttcctgcaat tcagctgtac gcgctttgac ctgcgccgcc
    22741 agctgttcgc ggtggcgatt cagtgcatga acactgctgc gaaacgcatc catcagccgc
    22801 ccgatggtat ccagctcccg tacgccagcg gtttccggga aaggggagtc aatatcaccg
    22861 tccagcagcc gttgcagcgc ctgcgtttgt tcggcaagtg gacgcgtgac tgagcgataa
    22921 accacgcgcc aaaggatcag aatcagtgcg caaagtgaaa ccatccccag caataacagg
    22981 ctgtattgcc cgcgtgcact ggctttttcc agatgcgcca gtccgtgctg attacgcagc
    23041 tcaatggtgt cgaccagctg actgacttcg ctactaaact gcgcgaactg ggcgatgtta
    23101 ttttgtgcga gagtttgtag gtgagtgctg atttcactgt cctgctgata cagcgccagc
    23161 aaatcgctat attggctaac ggtagttaac gttgttgcga cctgcgcacg aacacccgga
    23221 tcttcaatgc gtatttgccg acgttgcaga attttcaccg cattattgag ctgcttttcc
    23281 agcgttggtg catttttctg gatctgctcc agccccagat tcatcaccat ttgctgcacc
    23341 cgcagagcgc taaggcgcag ttcattcatc tggttaacat actcaagatc gatatcaatc
    23401 agccgatcga gtgcactttc agcagcctga cgctgatctt gttcgatcaa atcgtaaatc
    23461 ccggcctggg tcgctccagc ggaagttgtc gcattattcg cctgaccttg cgccaggcgt
    23521 gcgatctcat cggcggcagc gactatctgc tgactgagtt gctgttgttg ctggcgtagt
    23581 tgcagacgcc gccccaccag ttccccttgc tgacgtaacg aacgggagat ctcctgctcc
    23641 tgttgttcaa tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta
    23701 atcttcaggc tttgtgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg
    23761 gcactggtca ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc
    23821 gccatattca tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag
    23881 cgcaggttgt accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag
    23941 cccatccaga gtcttcgggt cagggttaaa ttcacggtcg gtgcacttta ggtgaaaaat
    24001 gttgagtcgc aaagcggaat gcatctagca taaagcctta ttattgatga ggctatcatg
    24061 cgcgtactgc tatttttact tctttccctt ttcatgttgc cggcattttc ggctgataac
    24121 ctgttgcgct ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc
    24181 aaacgcgcat ggaaactgtg cgcgctttat cccagtctga aagattcata ctggttatcg
    24241 ttgaactatg gtatgcagga ggcagctcgc cgttatggtg tggatttaaa agtgctggag
    24301 gcaggcggct acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg
    24361 ggcgcagagg ccattttact cggtagtagc acgacctcat ttcccgatct gcaaaagcag
    24421 gtagcaagtc tgccggtgat cgaactggta aatgctattg atgctcccca ggtgaaaagc
    24481 cgcgttggtg taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg
    24541 gcgcacggta aaccactgaa cgtgctgttg atgcccggac ccgataatgc cgggggcagt
    24601 aaagagatgg tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttaat
    24661 attgcgcttg gtgataacga tattgaaatc cagcgtaacc tgttgcagga gatgctggaa
    24721 cgccacccag aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt
    24781 gaagggcgta acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg
    24841 tatcgcgggc tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag
    24901 ggggaactgg cggttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat
    24961 gtcagcccac cgattttagt tctgacgccg aaaaatgccg accgtgaaca tattcgccgc
    25021 tcgctgtcac cagggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag
    25081 aaataacctt caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga
    25141 cgaattaaca catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata
    25201 cgtagcagac gttcacggct gagaatttcg cccggattcg tcacaaatgc caccaacatt
    25261 tcatactctg cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac
    25321 acattcaggc aataaccggc aaagcgatag cagttgtcct gagtgtgcgg ttgagcttgt
    25381 cgcgcgaggt cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc
    25441 agcggtttgg tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga
    25501 tcgctgcgtc cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt
    25561 aacatcaggc cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga
    25621 ttctgcataa tttcccgcag cccggcaccg ctcgctgtaa cggaaacggt atacccctcc
    25681 tgagtgaagt aagattgtaa tcgcgcctgg gtaaccggct catcttcaac aataacaatg
    25741 tgatgtggca tcagagggtt ttactcattc tgttcatatc tgttcatatt ctgccgtaag
    25801 ccgttcatcc tgaccagtgc cgctgttcat atttgctcat taagatcgct tcactaaacc
    25861 ataattttac aggggctatt atgcggaaac tctggaacgc gctacgccga cccagtgctt
    25921 gttggtcggt actggcgctg gtcgcaattg ggattgtgat tggcattgcg ctgattgtat
    25981 tgccacacgt tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt tgccacagta
    26041 tgcaaccggt gtatgaagaa tataaacagt cggtgcattt ccagaacgcc tccggcgtgc
    26101 gagctgaatg ccatgactgt catatcccgc cggatattcc aggcatggtg aagcgcaaac
    26161 tggaagcgag caatgatatc taccagacct ttattgctca ctccattgat acacctgaaa
    26221 aattcgaagc caaacgcgcg gaacttgccg agcgtgaatg ggcgcgaatg aaagaaaaca
    26281 actcggcaac ctgccgctcc tgccataact acgatgcgat ggatcatgcg aagcagcatc
    26341 ctgaagcggc acgtcagatg aaggtggcag cgaaagataa tcaatcctgc atcgactgtc
    26401 ataaaggtat tgcccaccag ttaccggata tgagtagcgg cttccgtaag cagttcgatg
    26461 agctgcgcgc cagtgctaat gacagtggtg acacgctgta ctctattgat attaagccaa
    26521 tttatgcggc gaaaggcgat aaagaagcct ctggttctct gctgcctgct tcggaagtga
    26581 aagtccttaa acgtgacggc gactggctgc aaattgaaat taccggctgg acggaaagcg
    26641 ccggacgtca gcgtgtactc acccaattcc caggtaaacg catctttgtt gcctcgattc
    26701 gtggtgatgt gcagcagcag gtgaaaacgc tggagaaaac caccgttgcc gacaccaata
    26761 ccgagtggag caagttgcag gccaccgcgt ggatgaagaa aggcgacatg gtgaacgata
    26821 tcaaaccgat ctgggcctat gcggattcgt tgtacaacgg cacctgtaac cagtgccacg
    26881 gcgcaccgga aatcgcccac tttgacgcta acggttggat cggcacgctc aacggcatga
    26941 ttggctttac cagtctcgat aaacgtgaag aacgcacttt gttgaaatat ctgcaaatga
    27001 atgcgtctga caccgcaggt aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa
    27061 taacgatctc tttcaggcat cacgtcggcg ttttctggca caactcggcg gcttaaccgt
    27121 cgccgggatg ctggggccgt cattgttaac gccgcgccgt gcaactgcgg cgcaagcggc
    27181 gactgaggct gtcatctcga aagagggcat tcttaccggg tcgcactggg gggctatccg
    27241 cgcgacggtg aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc
    27301 gtcgaaaatg atcgccggat tgtcggatca cgtacacaac gcggcacgta ttcgttatcc
    27361 gatggtacgc gtggactggc tgcgtaagcg ccatcggagc gacacctccc agcgcggtga
    27421 taaccgtttt gtgcgcgtga gctgggatga agccctcgac atgttctatg aagaactgga
    27481 acgcgtacag aaaactcacg ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac
    27541 ggggatgttc cataacgctt cggggatgct ggcgaaagct attgccttgc atggtaatag
    27601 cgttggtacg ggcggagatt actctaccgg tgctgcgcag gtgatcctgc cgcgcgtggt
    27661 tggctcaatg gaagtgtatg aacagcaaac ctcctggccg ctggtattgc agaacagcaa
    27721 aaccattgtg ctgtggggct ccgatttgct gaaaaaccag caagcgaact ggtggtgccc
    27781 ggatcacgat gtttatgaat attacgagca gctaaaagcg aaagtcgccg ccggtgaagt
    27841 tgaggtcatc agcatcgatc cggttgtcac atccacccat gagtatctgg ggcgcgagca
    27901 tgtgaagcac attgcggtta acccgcaaac tgacgtgccg ctgcaactgg cgctggcaca
    27961 tacgctgtac agtgaaaacc tgtacgacaa aaacttcctc gctaactact gtgtgggttt
    28021 tgagcagttc cagccgtatc tgctgggtga gaaagacggt cagccgaaag atgccgcatg
    28081 ggctgaaaaa ctgaccggca tcgatgccga aagcattcgt gggctggcgc ggcagatggc
    28141 ggcgaacaga acgcagatta ttgctggctg gtgcgtacag cgtatgcagc acggtgaaca
    28201 gtgggcgtgg atgattgtgg ttctggcggc gatgctgggg caaattggcc tgccaggtgg
    28261 tggctttggt tttggctggc actataacgg cgcaggcacg ccggggcgta aaggcgttat
    28321 tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgactacaa
    28381 aggctacagc agcactattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa
    28441 agtgatcaac tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc
    28501 cggaactaac ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa
    28561 gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat
    28621 cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc
    28681 caaccgtggc attatcgcca tgaaacaggt ggtgccgccg cagttcgagg cgcgcaacga
    28741 ctttgatatt ttccgcgagc tgtgccgccg ctttaatcgc gaagaagcct ttaccgaagg
    28801 gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg
    28861 acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt
    28921 tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc
    28981 gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgctg atatgaacta
    29041 cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc
    29101 tggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc
    29161 gcagttatgt gagtcggaaa ctctgcgtca gcaatatacg gtagcgggta aagagccagt
    29221 gttcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt
    29281 ctttaacgct cgcggtcagg tgttggcagg ggcagtagtt tctgaccgct atgcacccgg
    29341 cgtggcacga attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc
    29401 gctgtgcaaa tacggtaacc ccaacgtgct gaccatcgac atcggtactt cgcagctggc
    29461 gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag aagtacaatg gagcagtgga
    29521 gcaggtgacg gcgtttaacg gccctgtgga gatggtggcg cagtgcgaat atgttcccgc
    29581 gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg
    29641 ctggcgcagt tgttctcccg tgaactggac gatgaacaac tgacgcaaat cgccagtgcg
    29701 cagatggctg aatggttttc gttgctgaaa agcgaaccgc cgctggctgc ggcggtgaat
    29761 gagctggaaa actgtattgc cacgctgaca gtacgtgacg atgcccgtct ggaactggcc
    29821 gctgactttt gcggcctgtt tttgatgact gacaagcagg cggcgctgcc gtatgcatcg
    29881 gcctacaaac aggacgagca agagattaaa cgcttgttag ttgaggcagg gatggaaacc
    29941 agcggcaatt tcaacgaacc ggcagaccat ctggcgatct atctcgagtt gttgagtcat
    30001 ctgcattttt cgctgggaga ggggaccgtt cctgcgcgaa gaatcgacag tttgcggcaa
    30061 aaaacactga cggcgctgtg gcaatggtta ccagagtttg ctgcgcgttg ccatcagtat
    30121 gacagctttg gtttttacgc ggcactaagc cagttattgc tggtgttagt ggagtgcgac
    30181 caccaaaaca gataacgtcg tttatgcgcc tgaaaagacg cgtttagcgt cgcatcaggc
    30241 attatggcgc agttgccgga tgcgtcatca catccggcaa acacacatca cggatgagct
    30301 acaaaccggg aaagccgctg gcgtagcagg cggttttcct gcttcaggtg tgcaatatca
    30361 tccattaacg tcagcgccac cgcgatcccc ggccagtcca gagccagttc atgacgcagg
    30421 cgtaccgcgc gttgcaccac aatggcggca tggtcgtcaa atacccaggt tgtttcctgg
    30481 atctcacgcg gttcaaccac ccccaaaccg acaatttcat tcaactcctc ttcagagatg
    30541 ccggtatgca ggcaaaattc ggtaatagta aaagtcaccg taacattagc catcatgctt
    30601 tcccccaatc tttacgtgga tcaaaagacg actgggcgtc tgccagttgc tgccacagcg
    30661 cggcagtgtt ttcatccggt ttcggcggca tcacgatttt cagtaccgca tacagatcgc
    30721 cggtctgttt tttgctcacc agacctttgc ctttaacgcg caatcgttgc ccggcctggc
    30781 tgcctggcgg gatagtcagc aaaatgcttt ctttcagtgt tggaacggtg actttagcac
    30841 ccagcgccgc ttcccacggg ctaaccggca ccacaatttc cagatcctgg ccgacaatat
    30901 cgaacagcgg atgtggcgca atatgaatca ccagccacaa atcgccattt ggaccaccgt
    30961 tttcgcccgg cgtcccctgg cctttcagac ggatgcgttg accattgccg acgcccgccg
    31021 ggatcttcac attcagcgtt ttcgggattt cctgttcgat catgccaaag gcgttataaa
    31081 ccggcaggtt atagctgatg gtacgcttat gctcagtcag cgtttcttcg aggaataccg
    31141 ccacttcgat ttcaatatcg tggccgcgtg cggcggggcg ttgacggctc tggcgggcat
    31201 gctgaccgaa aattgacgag aagatatcgt caaaatcttc ggcattaaaa ctctgaccgt
    31261 cgccatgatg gaactgacgg ttaaattgcg gatcgttgcg atgttgccac atctgatcat
    31321 actcagcgcg acgttgttca tcacttaaca cttcccaggc ttcagcgacc tctttgaagc
    31381 gggcttcggc atccggttct ttgctgacat caggatggta tttacgggcc agtcgacgat
    31441 aggcggtctt gattgtcttg agatcgtccg tcggtttcac gcccatgatg gcgtaataat
    31501 cctttaattc catagcgtta tctcgcgtaa atcaacacaa attgaaggaa cccctgtaag
    31561 gtaactccta taagtgtagg gtaatcctca aaatttcata tgccaacaca gaatatgtta
    31621 ttgaaatcat cgcggagagg aggtcgccat caagatgggt tgctgaacat attttaaaca
    31681 ggtgaaaaag ggtgagcgat ttttgatagt tgaaccaggc actttaagtt taactagggc
    31741 gtcattattt attaaatttt atagacgcta tatatgggta gtaatataca tggaattagt
    31801 tgcactgcaa ataattattt gaaacaggcc tggaacgata taaaaaatga gtacgaaaaa
    31861 aatcaaacat attcaatcac gctttttgaa aacacactgg tgtgttttat gcggttatac
    31921 aatgaactca gacgtaaagt aaatgaagag gatactccat gtctggaatg tgaatcacta
    31981 gaaaaagaat ttgaggaaat gcagaatgat aatgatctat cattatttat gagaacattg
    32041 cgtactaatg atacacaaat ttattcaggg gtttcagaag gtattacata tactatacaa
    32101 tatgttcgag atattgatat tgttagagtg tccttgccgg gcagagcttc agagtctatc
    32161 acagatttta aaggttatta ttggtatggc tttatggaat atattgaaaa cattaatgcg
    32221 tgtgatgatg ttttttctga gtgttgtttt gatgatgaaa atataagtgt ccagccagag
    32281 cggataaata cgccgggaat atctgatttg gattctgaca ttgatttgtc tggtatatct
    32341 tttattcagc gtgaaactaa ccaggcatta ggattaaaat atgctcctgt agatggcgat
    32401 ggatattgtc tgttaagagc tatactggtt ttaaaacaac atgattattc atgggcgctg
    32461 gtcagttata agatgcaaaa ggaagtttac aacgaattca ttaaaatggt tgataaaaaa
    32521 acgatcgagg ctcttgttga tacggcattc tataatctca gggaagatgt aaagacgtta
    32581 tttggcgttg atctacaatc tgacaaccaa attcaggggc agagtagtct tatgtcatgg
    32641 agctttctgt tttttaaaaa acaattcatt gatagttgct tgaataacga aaaatgtatc
    32701 ctgcatttac ccgagtttat atttaatgat aacaagaact tgcttgcttt agataccgac
    32761 acgtcggata ggattaaagc ggtgaagaat tttcttgctg ttctttcaga tagcatttgc
    32821 tcattattta ttgttaatag taatgtggca tcaatctcct tggggaatga atccttttca
    32881 acagatgaag atcttgagta tggttattta atgaacactg gcaatcatta tgacgtttac
    32941 ctccctcctg aactttttgc tcaggcttac aagttaaaca ataaggaaat gaatgcgcaa
    33001 ctcgactatt taaatcgtta tgcaatttac tgacaagggc atgtgctaaa aaccattgtt
    33061 attagcctca cttctcttat tattggtact attgcctctg tattagtaac gccgctgata
    33121 tcctgtttag ccacaggtgt aatcatcagc ggtgtacgcg aggcagggga taagcagaca
    33181 tagcttgcat caaaccttgc ctgtttttga agatgtatat agaaaaacag gcgttcaaca
    33241 agccattttg cgaacctatt cccggaaaaa agtcatattt ctgtcacact ctttagtgat
    33301 tgataacaaa agaggtgcca ggaatgaaca aaacgctaat cgccgcagct gtggcaggga
    33361 tagttttact cgcttcaaac gctcaggcac aaaccgtacc ggaaggctat cagctacagc
    33421 aagtgctcat gatgagccgc cataacttac gtgcgccgct ggcgaacaat ggcagtgtgc
    33481 tggagcagtc gacgccgaat aaatggccag aatgggacgt ccccggtggg caactcacca
    33541 ccaaaggtgg cgtgctcgaa gtgtatatgg gccattacat gcgtgaatgg ctggcagagc
    33601 aggggatggt gaaatcgggg gaatgcccgc cgccggacac cgtttatgcc tatgcgttta
    33661 tgcctatgcc aatagtctgc aacgtaccgt tgcgaccgca cagttcttta ttaccggcgc
    33721 attcccgggg tgtgatattc ctgtgcatca ccaggaaaaa atgggcacta tggacccaac
    33781 ctttaacccg gtgatcaccg atgattccgc cgcattcagt gaacaggcgg tggcggcaat
    33841 ggagaaagag ctcagcaaac tccagcttac cgacagctac cagctactgg aaaaaatcgt
    33901 taactataaa gattcccctg cctgtaaaga gaaacaacag tgttcgctgg tggatggcaa
    33961 aaataccttt agcgccaagt atcaacaaga accaggtgtt tccgggccgc tgaaagtcgg
    34021 caactcgctg gtagatgcgt ttactttgca atattacgaa ggttttccga tggatcaggt
    34081 ggcctgggga gaaatcaaat ctgaccagca gtggaaggtg ttgtcgaagc ttaaaaacgg
    34141 ctaccaggac agcctgttta cctcaccgga agtggcgcgc aatgttgcga aaccgctggt
    34201 cagttatatc gacaaagctc tggtcaccga tcgcaccagc gcaccgaaaa ttacagtgtt
    34261 ggttgggcac gactccaaca ttgcctctct gttaacggcg ctggatttca aaccgtatca
    34321 gttgcatgac cagaacgaac gcacgccgat tggcggcaaa atcgttttcc agcgttggca
    34381 tgacagcaaa gccaatcgcg atttgatgaa aattgaatat gtgtatcaga gtgcggaaca
    34441 gttacgtaat gccgatgcgt taaccctgca ggcacctgct cagcgtgtga cgctggaatt
    34501 aagcggttgc ccgatagatg ccaacggttt ctgcccgatg gataaatttg atagcgtgtt
    34561 gaatgaagcg gtgaaataac agaaaactcc cccgcgagaa gcgggggagt cgctggttaa
    34621 acgtttttac gttcgatggt ctgttcgccc caaaaaagcg aatctttatc ggtcttagca
    34681 aaggctttga ctaacacttc atcgctacct tcttcccaaa tcttttccgc cattttttcg
    34741 tcgtacccgg cgacttcgaa aatggcctcg gctatttccg gcgacgtatt gcgcagagat
    34801 gcccattcac cgacgtgatg agctttcgct tcttgagttg gcatgcgtat cctcctgttg
    34861 aagattagcc gttaagttta actgccagac ctgcgacata ttccccttga taacgagcaa
    34921 tagacagttc ttcctggctt ggctggcgtg agccgtcacc gcctgcgatg gtggttgcgc
    34981 cgtacggcgt accgccgcga acctgtgaaa cgtcaaataa ttcctgcgct gcgtagccaa
    35041 tggggacaat taccatgccg tgatgcgcaa gggtcgtcca ggttgaagta atagtttgtt
    35101 cctgaccgcc gccagtaccg gtggaactaa agacgctcgc cagttttccg tatagtgcgc
    35161 cggaagccca caggccgccc gtctggtcga ggaaggtacg catttgaccg gacatgttgc
    35221 caaagcgggt aggtgtacca aaaataatgg cgtcgtaatc ggccagttct tgcggggttg
    35281 caaccggtgc agtttgcgtt ttaccgcctg ctttttcaaa taattgcggc ggcatggttt
    35341 ccggtacacg cttaacgaca acttctgcgc catccacttt gcttgcaccc tcagcgactg
    35401 cgcgtgccat cgtttcaaca tgtctaagga tatggtttat cattttgaaa aatataattt
    35461 tatttcatcc tcctggtcac tttggggcac gtctggggca cgggcattaa ggacattatt
    35521 caacatggca acttgagtca cgctgcactc aggcatccat gcaccataaa cattgtagac
    35581 catgctggcg ctggagtgcc ccatctgtga tgcaataaat gtcgggtttg ctccggcaga
    35641 taaagcccag cacgcatagg tatggcgtga ctgatacgct ttacgggatc ggatacccgc
    35701 tctttttatt gctgaatccc atgtcgctcc gatggagctt accgcgtagt taataccagc
    35761 cttgtgattc ttgcgaacga tttgcggaca gaaaacaaaa gtgcactcgt gcaaaattgt
    35821 tcttccgtac tcgcgtaatt gaacagtgat ctgatgctgc ctgctaagac gagtaagcat
    35881 cgcctggttt ttaagtgctt caattgctgg tgccagaaga tgtataaccc ggttagtgcc
    35941 tgcgtcggtc tttggtagcg taaaatcacc tatttttgta aaatttcgtc gcactgttat
    36001 cgtgccagct ttcaggtcga tatcctccca tgcaagtgcg gcaatttcac cgtgtcgcat
    36061 ccctgtaaaa acagccactg tccagaggtt tttggtctgt tgatgatggc aggcatcaat
    36121 gagacggcta aactcatctc tggtcagtgg atccggcact ggttttgatt tcctcagcgg
    36181 tgttattgaa ttaaacgggt ttttctccag atacccgttt tcggcggcaa agctgaacat
    36241 tccggctgtt gttgtcatgt aatagttcac tgtgggtacg gttcttcctt ttcgggacgt
    36301 gctggttttc ctgcttccct tttccccggt cagtaaatct ttcctgataa acagcagatc
    36361 ttcttttgta atcgatgacg ccagcctgcc aggaccaagc ctcggtagca tatttttcat
    36421 cactgattga taacgattta aggcgttact actgatttcc attgctttca gagtaagcca
    36481 tttctgtgcc agttcaccga cggtgatatc tttttttacc aggccaaata gcttcaggtt
    36541 aggtgagtca gggaatcgct cggcataatc aaacgttccc gttctgattg caaagcagac
    36601 agaagccctt aactcaccag cgatctttct gtttttcgga gtatcgggca cgcgaagatt
    36661 ttcgcgcact cgtttacccc tgtacttaaa cgttattcgg agttttcctc cgtgattttc
    36721 aacgccggct ggataggctg aattcgccat tgttcctcct gcgtccaaga gcacgctcag
    36781 gttatactct tatggacaag aattaaacat cctgagatgg taagggttgg tttttgatcc
    36841 aagtgttgat ggtaggaagg tgatagagac attcgctgtt tttctttggc gttccgtcgg
    36901 gggcgatttg tttatattct ctgccgttca tccacgcact gtccctggcg cgtaatattg
    36961 ttccttttct caggcctgtt acagccatta aaagctccag tgttacccat tcattcgcat
    37021 ttatctgtac aacaggtgtg atgactggct gaatgccatg gttatgttgg tttactaact
    37081 cgcgcataag ttgcctctgc atgtgtaaga aaaaaccgcc cgaaggcggt tgtcagttga
    37141 ttgatgtacg gcgcattttt cgaaggctgg caatatgctt ttccttctca atttccgctt
    37201 taatcatatg cagttcgttg tgctcgattc gctcaaattc ttcattaaat gcactaattg
    37261 aagtggctct ggttctgccg tcgagccttc ggtagatcac ctgagtcaga gttaccttgc
    37321 atatttctac cggatagttg ttggcatcaa cgaaagactg cccgcgctgg atcaggacga
    37381 acactggttg tattccttga gtgccatatc ataaacacgg catgccgaga ggtgtgttgt
    37441 agtcaacgtc gttcgtatcc tgaaaagtcc atgtgttgtg acggtaactt ccggggcttt
    37501 gagtttcact gcatcaacga tatgctgatg tttgcgcctt tcgaatatag agctggatat
    37561 aaccacactt gctgttaaac cgtaactttc aaatgtgatt ttcatgtcat tatcctcgca
    37621 gttagccggg caacaaaaat aaataatgtt gttcctgcga ataacaggat accgcccgtc
    37681 attagagaaa ggaaaaacaa tattaatgta cgcgggttgt ttctcaaaat attctctctg
    37741 tgattcgctc actctgacaa ttcgttgatc aagtcacggt atttattcag ttcttgcaac
    37801 gccttacatg caccctccca tcgtttctgt ttccgcccgg cgcggcgcgc ttctttccgt
    37861 gatttacgta gcaggtcacc tataatgttg tgctgcactg gctgcactgg ggcgatattt
    37921 ggttttttgt ccacttccct tgcaactgcc tgccagatgc cattttttac agttactacc
    37981 ccctggtttt ttaacttcca tagagtgtca ataacgtcat tcaaatcaat attgagacct
    38041 ttagaaatac tttcggctga tgcttttcct aatttgttca attctgtgag cactgcgttc
    38101 atttttttct cctgatattt taatgtcggt ggggctgtta tacggttagt gaggtgtcag
    38161 cttcttctac cagcttttcc agttcatcca gctttcggga aagaatttca ccaaacagat
    38221 gaagttctgc atctgctgta atcgggattg gaacaaatcg tattccactt ctggcaagtt
    38281 gatttgcgat ttccagacac tgccttaatt caactggtga tgcctttgtc aacgtcgttt
    38341 tttcgctcat tggttttctc cagaaattta acaatacccg gaaccatctc aatggatggt
    38401 gtgccgcatt ggttacccca tacatcaaat ccatgcgagg tgtggcgggc gaacagttct
    38461 atccggggaa catcgccaag aagttgcaca agcttttctc gagccatatc cggcttgcgg
    38521 gagtgatcaa ggcgcggtgc agtgaatgac tggattattc cggcgttaat gcgttcgggc
    38581 agatttcctt tcactgcaaa aaggcagtct tcgctgttag cgcgagtggt gctacccata
    38641 cccatgacca gcttgtcggt ctgtcttttc ccgcatttgt tccaggttaa tcctttcatc
    38701 gtcaccagac gaaagcccca cgcttctaca accttcaatg cttcaagtgg ttgtgtaggc
    38761 acccaccaca tagacaacag gcaattatcg gctgccagtt cccataccgg gaggcggcag
    38821 atatccagaa gactcatgac cggatattta aaacttgcac cgcgttgacc atcggttgct
    38881 ttgtcgcgga atgtccaggg gggatctgcg taaatgaggg tgtatttttt cgtcatagcg
    38941 cgttttcaaa ctcatcaatg taaagtcctg ctttaatcag tcggcgacgg cgttcggcct
    39001 tttgcacgta ttcctcgcga cagttttttg ctgcgtgttc acggcttctg cgactgaatg
    39061 gaggaacggc attgcgtgag cgaggtgtgt cacgacgagg atttgattcg agggagaaaa
    39121 agcgatcaac gattccggtg tctgtggtga atggttctga agcctttatt ttcacaacct
    39181 tactgccctt tgtcaggccg cgagctacct tgttgaactc gcaaagtgaa acaccaaatt
    39241 tctctgctat ttcactgcca gtaacaggac gaccacgaga ctgaatcatc caggtgacgc
    39301 gttcttttaa cccacggaag gcaccgggcc ttccagaacg cctgtagaaa gcgatctgtt
    39361 tcaaattttt gtctccagat atgaaaaccc cgacgggatg tcggggatgg taggggaagt
    39421 tacaggctta acgagaagcc ttttcgggaa tttcgatctg acaatcttga aatatcctgt
    39481 agcgtgcggc gcatttcttc cgtcagatgc ttgaaggcgt taaattcagc gacagcggcg
    39541 tcaacgttat agcctttgct gtgtagctca ttgaggatgc gcatagttgg gcttggcatg
    39601 tcgaagagca cagaaacgtc caggttaaga gtttttcgat caacatagct catcatggag
    39661 taggggtggt gctctgaaaa ccacgataga gggtagttga tgttcattgg tgcaggtaaa
    39721 aggctctggc gaagtttctt ttcgcactcg atgaagtatc ggcggatctg gcggcctttt
    39781 tcgttacgct caaccatcgc cagttctttg gctgtgtcga gagttaggtg gtagtccttg
    39841 ctacggcgat cacctcctcg ccctttggtt tgcccatttt ggcaaatcaa aatatagtcc
    39901 tggctttcaa tgaatccata ttcagcaatg cgttctttaa cccagtgcgc gaacattctg
    39961 cccacaccaa gaaaagagtg tagatcacga gcgttaacta gaagaattgt ttcgttgtcg
    40021 atagtgccgt tgaatacggg gatgagttga ctggtcatga tgacctcctt tgaagtttta
    40081 gttagcaatc accagttagt ggctggtgat cgggtgtcaa ctagagcctt caaagatgct
    40141 ccgggcatat tccccttgcg ggtattgtat tacgcctctc cacccgacct ttgtacggat
    40201 gtgactatgc caaattgcag gcataaaaaa gccgcaaagc tatcgggtgc ggatgaccgc
    40261 tttgaagttc tagtgcggtc agtatgcgat agctctggcg gatttgtcaa atcatgtagg
    40321 ccttatcttg ctgtaagccg cgccattcgg gcttttcccc agatttgggg gaaactatct
    40381 gcgaagtatt cacctttgac ggcaagttgc aggttagcca cggttaacct cctgcggcgg
    40441 ttctggtagc ggcatccagt gtgttacctg ctcaacacca ttgcccggct taatcgttgc
    40501 ttctcctcgc cgaaaggtgc ttccggtata gcgtgcggag catattagcg gctcaaccag
    40561 atcactatcg aaattcaccg aaataagtac gttctgattc tttttcggca ttcgctcact
    40621 acagcttatc caactatccg gagttaccgg atagttgccc gatagtgcat tctgctccag
    40681 tgatgctttt acaaaccacg ctgcctgaac tataacgcca tgaatccagc gcaaatcagc
    40741 atcgcgatct ttctttttca tcttttcgcc acttaaggcc ttgcttatgt ggctgcgtac
    40801 caggtcttca tgtaattcct tcgcctcctc aatggtgaaa ccaccaggca gaagagccgg
    40861 agttaccgga gagctggttg acgcttccgg gattttccga aaattattgg ttgacgaatc
    40921 tttattttcc cgaaagtttc cggactgaag catggcttcg cggcaatcgt tccagcctgt
    40981 agcgtatgca gccgctttgc tgctgccttc aactggcgca tcctgccaat acatttcttc
    41041 cggcactatc ggcgctggag gggcggcaaa tagatatccg ccaaagtcag gaagctctct
    41101 aatggcctgt acgaattttt gtttgcctac gtcaacccct aatgggtaat gagctataat
    41161 ctttgccacc ggctctgctt ccagcgatgc cagcgcaatc cgtgccagct cttccgcttc
    41221 ttctgctgga agcacaacgt tgctacccgg tccgtatgtt tcgcgccaat gctggattgt
    41281 cagcagtcga tctttggtaa aagtggtcat agctatttca ccttaatctc aacatttcgc
    41341 agctttagct ctactggcag gtctgacttt cctgttaatg ctaatgcgag attttctgga
    41401 gtagtgagaa cagttattgt tttccccctc gccagacgaa taatcatgtg tatctcgcta
    41461 tcgtcacatg ctcctggtcg aacaattgag atttgtccgt tcatctcact ctcctttgat
    41521 gcgaatgcca gtagcgcgga ttgcatcgat gacttcagaa actttgtatg ccattaccgt
    41581 ttggtaatca tcgtgaaaat ctgttcgatg aagcatgctg ctacgttccg ggagcagtat
    41641 ttcccgcgct tccagttctg caatgcgctt ttttgctgct tccagttcat ccagtaattc
    41701 cagcacggta gccggattag ccttggcaac aaaatcccgg actggcttac aatcaatctc
    41761 cgcaatgggt tgatacgatg tgtagccatg ctgtcttgta taactaccgt gacgaataac
    41821 gaaaaaatca ccatttattt ttttagcctg ccacttatct tcaccggctt tctctgccgc
    41881 ttcacgcagt gcctgatagt caatcttgct cactggttgc ctcctttgcg aagccgttcc
    41941 gcccattctt caagggattt ctctgcatat tcaccggaca ggccatcaat cggatgcgat
    42001 tcattagcta actcttcttt tgctgacaga atcatgcgcg taacgtcgaa aacttcacgt
    42061 aaagacttat tgataaatcc gtggttgaaa gcagcagcaa gacggcttgc ggtatagtta
    42121 atcccctcgt tgcgagcctc cgcacgaatt tcagccagaa aagcatcggt ggctggaatt
    42181 tgcggcatcc ctccatctgt tgcgcagata tacgcatcag atagctcatc ctgctcgcca
    42241 tcaaacacgt agcaactctg tgcgataaat ttattcagcc ccgcattctc cgccgccaga
    42301 gccgaaaact tctcgtgtgc caacttaaca gccgaatcag cctgcttaat tgactcaatc
    42361 gctttctgtt ggtcttcggc cagcttcgct aaattagcct ccagttcggc tatgcgttcg
    42421 tttaatgcat ctcgttcatc cagtagtgac agcacggttt ctggcccggc cataatttta
    42481 aaggcgtgaa tcgcatcaat atcaatacca agctccttga gcttcttttc gcttaataaa
    42541 tcatcatcac ctggtaatat tgacagatgt gtcatcgctg taattgcgcg ttccgacacg
    42601 tcacgcaata cctgatagtc aatttcggtt gtcatatttt cgtctcgtta tgggagggga
    42661 attggttgta agctggaatt gccggatgtt tacccgtgtc cggcgcacga cctcacgttg
    42721 cagcgtacga gaatagacta ttgctcagta tcgtttgatg tcggctgagg acattcgcct
    42781 tgctctcaca acagagcaag aagcatggac accccctcac gatgtgatga ttggtgagtg
    42841 tcgatccctg aatgattgat gttgttaaac tcaaacatct tgctcctggc aatagcctca
    42901 acggtattcc agaatactaa tagttctgtt ttctgctctg ttttatcagc aacaggaagt
    42961 ttgatatgtt gttgacattt attaatgcaa ccattttgaa tatgttcctc gtgcttttca
    43021 ttgtcataag catacagccc gacaaaaaca tctcgcacgt tacgggaaat attttccaga
    43081 ttcgtctttg gttcctttat taaattaagc accgataaat gggctttaag cgtatccgca
    43141 gctattttct gaatatccgc tggtaaatct ttaaattgca tagtataccc tccaagaact
    43201 gtgtattttc tcgtgaaata tgagcgatat gtcgtgacgt ccctgtcaca gatttcatca
    43261 cactgagaac gtgccgatga aggttaagat atcttcttct tcgaaacgct ctttcagcag
    43321 gtcacggaac tctttggcaa tttcttcttc cgcagcttca acacgcacga tacgtagcac
    43381 cagaacaggc tgttcgcttg tcagaatgct taatcgcagc gtgaattccc tgttgcccag
    43441 gccctcgtat gggatgcatt caaagcggaa agtggcaggc atgatttctt tgcttttagc
    43501 ctccacggac tccatgactg agcggctacc gctgaagttt tgctcttcat attcagcact
    43561 gcgttttgca tcgatggtaa gacgacggac agcagagata gcctgtttga tatccagcac
    43621 attaccgtcg ctgtcatagg ctgtgaggaa gtcggcccag tcttccagcc attcagcgag
    43681 agatttctgg agttgttttt tgccgtcaac gtcacgcaat gcgcgatatg gtgatgtggc
    43741 tttcagttcc agcagtgcct tgttgtcagc gtgtccaggt tgctccagag tgccgatatt
    43801 gaacacagtg actgctttca tcgtttccgc gttaatgaag cagcgggagt gctcatcggc
    43861 ataccctttg ctgtattcaa caaagtcatc aatactggtt gtggtcatga ctccacggaa
    43921 acgaaaacgt tctgtcatga atcgttccag actttcgata tttacattgt ccggcaataa
    43981 cgcaacaggg cattccgtaa gcggcaaatt ttcaccagaa aaagcagtag tggtgagttt
    44041 tacaatttct ttaatggcat tgctatctaa ctgagacatg tatatttacc tttaatttat
    44101 gtatttaggg ataatgcgcg agcgttttaa ggcggggaat attcagcgta aaacagcgtc
    44161 atgttggccc tgtttatcaa aaagctgtcc ctggtctttc tggaacaaag taagctcacc
    44221 acctttattt acaaacatcg gggtgttagt tgtgtcttct tctgatttac taccacgcat
    44281 tgtcgggcgt gtgaatttta gtttatggga aactgatacg cgattttcat caagtgaaga
    44341 taattcgaat tcaacacata cctttccttt tttgtttgtg ctgtttacgc caaaggcaac
    44401 ttcacttaat accgcgccaa gtttgttaac aaataccccg ccgtcgaggt cgttaatgaa
    44461 aacattaaca tcagtttgat gctggttcat attaatatct ccgtgtagag gtgtgttaag
    44521 gcttcagttg ttcactgcat ttaaagcctg gttttgtttg tgaattaaaa actatcgctg
    44581 ccgtactgcc agtaacctgg gtcgtccgtg tgattcgggt ttacgttttt gctgcgattg
    44641 tctttgtctt tgagagtgga aaccatattt gctaccgttt ctggtttctt tccttcagct
    44701 ttttctttca gtgtctgccc tgtttctgca atgaatggaa tgcggatttc catctggtaa
    44761 ccgtctgcgc cagtctttct gtttgtggtt aatactttct ggaggacgag tccgattttc
    44821 tttccgtgaa actcaggtgc aacatattta ctgacggaaa ccatatgttg cgttaactgc
    44881 ccaattcctg tgcatcccat catggcgtga ataatgctcg ccccaaattt attttcagta
    44941 ccgtcattct tctgaacaca aacactgaga tattggattt tccgtccgtc gtcggattct
    45001 ccagaaaact caatgaatcg ggcacctttt tctgattgct tgagttctgc ttcggtgatg
    45061 ttaatgatat gcgcacccgt ttcggtaata aaactacctt gtccggcggt cagtgctgat
    45121 tcttcgttat aagtaaaaat cacttcatcc attagttatt aaccccccat tgttctccaa
    45181 atacaaaacc aatttccgcc agtgcttcgt ccattttgtc gatgaactcc ggcaccatct
    45241 catcaaaact cgccatgtac ttttcatccc gctcgaccac gacataatgc aggccttcac
    45301 gcttcatgcg tgggtcgtag ttggcgaagt accaggcgtc cttgtctgta acccacatgc
    45361 tgaattgcac ctgggccatg taagcaggct tgatagcgtc aaagccgcca agccggaatt
    45421 tcatgaagtc gcgggaagta aaagggcatt taagctcaag accgttaccg tcgctgcata
    45481 aaccgtcggg agagcaggcg gtgcgcatgc tttcgtcacg aaaaattatt ggcgtttccg
    45541 agacggtaac atccgcgata aattcgaaga gggcacgggc atcatcttcg tattgctttc
    45601 cccatgcgag cgacttagcg ttaacttccg gtgccacgcc agtacaaact tcagccagca
    45661 gggtgtggaa gtatgacatt tttgtatcag gccatttgct gccggaacgt ggctttgcta
    45721 tcacattgtg aacttctgaa gcagtgataa caccgagtct cagcttgtgc catccatcat
    45781 cgccctggtc gaggctggta atatccacgc cagttcgctg gagaataatt tctggtgtca
    45841 tgacacggcc tcgctgttat tttccgtggt ggcgtgaatt ttcgcttccg ccgtggcctt
    45901 gtttctggca gctttctttt tgacaaaatc aagcgttttg acagcttctt cctgactgag
    45961 atattcatgt gatgcaatcg tgcgacggaa tgttttggaa cataagggaa gcaggtcatc
    46021 ccatgtcttg ttaatttcgg tgattgcctg agtgatctca ttgataatct catctgatgc
    46081 gggagtgacg tcacgctcag ggatgtgatc agcattcagg atgatacctt caccgccctg
    46141 agtgttcagg tagtcgatag ctgtatccag gcgatcgcga cggggccagt atttgctggc
    46201 gcgcttaaca atggcttttc tggccatttc atcggggaaa ctatcccacg ggctacttcc
    46261 tccgttattt ccggctttgc tgcacgccct gatgacctcg atttccctgt tgctcatctc
    46321 ttcggtcaga tagtcgcctt cggatgtttt tacgacacaa taaccgccaa tgcgtgcgcc
    46381 cctgtctacg aaggggttgt atttgtgcgt gggggcgcag tcaataccgt tggactcgta
    46441 aatgtccttc tcgtaaacaa gtttgcattg cccccactga atggctcctg tgacctgtgc
    46501 cagatgcaga agccccatat aactgatatc caggcataca gccttttttc gtgggaccag
    46561 atacgccagc ttgctggccg ggttcagggt tatgccgatg gcggcaacat tgatgatagc
    46621 gttctgtgcg ctgggcagat ttgcccttgc tgtgtcagcc agaaaggcat ttttctggaa
    46681 ttgctgaatt gcaaactgac tttccttcgc ccatgtcagc gtcggttcag ttaatgcctc
    46741 gcagaaaaag cgctcctgct gcttaacaaa ttcaacgata tcgaacattt tttggtcctg
    46801 aaaatcagaa aggacagggg gagaattttc tctcccattc ttcttccgcc cgagcatagg
    46861 cgatcgctga gatataatcg ttgtacgcct cttcagcttt ttcgccagtg agtgctagtt
    46921 gggcttcttt gggtaaaaaa aggctgctca taagcaatgg tttatcgggg aacatgctga
    46981 taagctcctg cgcccgatca tcaatccatt tatccttttc atcctgaatt tgctgattaa
    47041 tccagcgacg ctcctctatg cggtcgcagg tgaggtatgc gttcatggcg gaactcctga
    47101 ttccggttaa tgcattaaat taatttgtcg ggaaagctga catacagggc agttacattc
    47161 ttcctcctgc tccttagcga agaaatatgc agcggcctgt aatgcgatat cttctggatg
    47221 ttccgcgata aacataacat tgccttccgt atcaataaca gaaatagcct catcagacag
    47281 gacgacaaaa taggcgatga ttttatcatc cataaaaact tctcccatta tcgttcctgc
    47341 tggagttacg acgcttttta cattgatatt tattttttga ttgagcatga tatttccctt
    47401 caggctggtg agattaacgg ttggccttta ttgttcaggt aaacttctat tgcatctgag
    47461 ataatgcgaa ttttttcaat cagtgaatgt gcgtaaagtg cattattaac gttcgctgac
    47521 gccatgtaat aacgcccgtt gtaaagaatt gctgtgccgg gtttaacgtc ctcgcgagaa
    47581 actaatgcgg ttccgtagtg aggtttgagc atgacaaatc ctccggttaa ttaatccaga
    47641 tatttaattt aatccccgat atgtggtcgg ggatggggtt aattaaagat ttacgttgaa
    47701 accaaagcgg gaagactttt ctgatgtacg ggaaatatcc agcaatttac ggcgcatttc
    47761 ttccgtcaga tgcttgaagg cgttaaattc ggcgacagcg gcgtcaacgt tatagccttt
    47821 gctgtgtagc tcattgagga tgcgcatagt tgggcttggc atatcgaaga gcacggaagc
    47881 atcaaggctg ataaccttgc gatcaacata gttcatcgtg gcgtagggat ggttatctga
    47941 gaaccacgag agagggtagt tgatagatag cgttgattct ggaagctgaa gttgttctgg
    48001 gattttttga ctgaaatagc agtcttccag tttttcgaac acttcccacg cccgatcggt
    48061 ttcgagcatt tttgcgtgac gggctgcgcc gcgttctgtc cagaggatga gggagcgaac
    48121 gttacgggcg attttcacag aaggtcttaa agacatgctg tgcttaaatt cccgcaactc
    48181 ctcattctca agtaaaaagt agtgcttacc gataacaaat cgtccagaat tccgattgaa
    48241 attttttcgg atgtaatctg gttctgtgcc atataagctg gcaagaagct ctgtagtcac
    48301 cacaggtata ttactaaatg aaattatgga gagggactcc acggagatgg ttttcatgct
    48361 ttgcgtccta cttgatattt tgatttaccc cttttgagag ggtgaccggg cgctcaaaac
    48421 cgcaagtagt cggcgggcat attcccattg ctggtattgt attagccgca cgcccggtca
    48481 taaaccaaga agactggaca caaaaaaacc gcatgtctgt cgggcgcggt aaccgctact
    48541 tgctaaggtg ttttgagcac catgaggcag aatatatccc cgttaatgca gcattgtcaa
    48601 acgatctgtt ctttaaagac ttaacgagaa gccttttcgg gaatttcgat ctgacagtct
    48661 tgaaatatcc tgtagcgtgc ggcgcatttc ttccgtcaga tgcttgaagg cgttaaattc
    48721 ggcgacagcg gcgtcaacgt tatagccttt gctgtgtagc tcattgagga tgcgcatagt
    48781 tgggcttggc atatcgaaga gcacggaagc atcaaggctg ataaccttgc gatcaacata
    48841 gttcatcgtg gcgtaggggt gatgctctga aaaccacgac aaagggaaat tgatattcat
    48901 cgcaggagaa gacagggcca gttgtttctg ttcccatagt tgtttttcca tgcgatcgaa
    48961 ttcagcaatg taggcttcct tgaatgcagc tgcttttttg ccggtgaagc ccatcaccag
    49021 aaaaacgaag ccgtttttgg tgatttggta cattgggcgt tgttcgcctt tggcgtcggt
    49081 gtaggtaacg tcctcaaaat tgagggcgtt aaatttctct gagcattcaa tatttgcaat
    49141 ggcgcgcaat acgttatcgt gtcgcttgca aaaatattcg gcaacggcaa aggacgtagt
    49201 aacagcgcga ccattggaaa tggtgatttc aggttgagaa aggactggga tagtagccat
    49261 aatggcagcc tccttgattg gtgattgata accaccgctg gaggtagcaa gctcgctggc
    49321 ggtggactgt acagggttgc tacaactggc aatcaaggga accagcccga ccgaagtcgg
    49381 ccccatacag cccaccattg ataagatgtg cgtgtatgtc gatacaaaaa aagacgctgg
    49441 cgcgtctgta tcgcctcgat tgtcagcggg gtagcaatcc cgacacccgt tttatgaggt
    49501 gttcgccaaa tatagccccg acatcacacg cagtcaatac cgttctttct cagaaacgct
    49561 ttggcggtgc cgcctgcacc ccaaaacatt ccctgtattg gtcagcgcca acttcctgcc
    49621 agtgttgccc gttctcacgc cgttctcgct ctcgcgcggg gatactctct catcgaccgg
    49681 atcgcacccg atgatacagc acgtttacgt gtaggggtct aaacaggtca ttgacgctgt
    49741 aaagctccag attgttaaag agcattttgc ggcgggttaa gtcgcgccgt acgactgatt
    49801 tatgtagccc tgtgtaaggg cgcgatgttt ctggcttgaa ataaatataa cttgcggtga
    49861 ttttcatgta aataccgttg gtgcttttta agggcgagaa attattactt aactgatttt
    49921 taaagtgatt tattttttaa gggggcagat aacaggaggg gatatgcaaa gaaaaacccg
    49981 gcgcatgggc cgggctattt atctggtcct gttctcattc agtagagtag gggccaagaa
    50041 atcgcactcc gttgaagtga ataagatgag aaggtgcatc agccacccat acctctgttt
    50101 cccatgcgat ttcaccaaga tatcgcccca tgatggagcg atttggaaaa gcggtcacat
    50161 agacaagtcc ggctgttgat ccggcaaaca gcctggcaag ctcagcatgc cgcttcccat
    50221 caaccggtcc atgactggtg acagactcaa ccagtagcag ccagtttttc gcagtaaaat
    50281 gtagcaccac atctggcatt ttaccgtgtg aatccacatc aacacctagc ccggccagca
    50341 gtggagcgtc gaagtagccc cacttttcgc cagtgtcgcc agcatagacc agcacgctac
    50401 ccggagcaaa acgtggggcg aagtcctcta ttatggcacg gataagttcg ctgtgctcgc
    50461 cgggactgag ggttatttgc tggcccgcag caatttcaac agggatacga ttctgttcgc
    50521 gctccttagc atagcgggta accagcgttt cacgctcggc taaataggtt gcaaggctat
    50581 cgtgccatgc cgggctgccg aaagtgcgta gcatggacag agcagcaggt tcgatctgat
    50641 aaacagcctt cgggctgttc actgggcgat cgggcttgtc cggattgtag agggctactc
    50701 cagcagcgca gaactgatgc atagactggc ggcggaatgt ctcacgagta ttgggtgcgt
    50761 agaccttgcc gtagtgctcc cgaacccaat tcatgattgg tgtaatgccc acaagcggat
    50821 tttccgcgtc ggcccatgct tttcccggtg tgaggttcag gagtgccagc aaacacaaag
    50881 cagaacgctc attctgctgc gctctgggca ggcctaagga tgctataatt tgctgagcgg
    50941 cctcaatata atcattttgg ttgttcatgc agtcagagct cctagcttag cgtcaatatg
    51001 ttcttgcgtg agagtttctt gttgcatagc ccacttgcca agctctatca gagtgtcacg
    51061 gcttgggtac ttaatcatct tgaggtcggt tgcattgacc tgtgtatgcc cgttgaagcg
    51121 acggaaatac ttatccactg cggtagtgtt caggaacacg gccaagccac gggccagcgc
    51181 ctcgggtaac ccatgtttgt tctcatgaaa cacgttcatg tgattttcaa atcccaacac
    51241 tgaatactca ctgaaggtag caggatcaac gacgcttgct accactcggc gcttttcctc
    51301 cttcgacgag aaacgacgca ccacacaata aaaaccgttc gggtaaagcc acttttctgt
    51361 ctcgtcgttg cgcatgattg cgttgggttt cttcaaacct tccacaggcc agacagtgct
    51421 actcatactc aaatggctcg ggtagatcag gggaacagtt ccttcttctg gcatgctgcg
    51481 caaatgagct ttcagtcgga aatcaactat cggtccggtc gataccttca cgccaatatc
    51541 agccagcgaa cattgtacgg cagatgataa ctcaatggtg cttttctcga gcgtggtcgg
    51601 cacatgaata aaccgctccg aatcgtccgg gtacacaatc tggtcaaatg ggtgttcgta
    51661 ggtgactagg tcaaaaaagc tatcatcggt cgaagttgtg actgtaacag cctcctgacg
    51721 gcctccacgc tccaggcgga tgataatatt ttcctgcaac acatcgtcat ccctgaaagc
    51781 tttgctacgt gattcgaaca aatgaatatg gcggattgct gcccgctcaa gtatgaaatt
    51841 acggaacgga cggtaatacg gtccgttgca gaaactacgt gggattatgg ccactatctg
    51901 cccgccaggt gcaacctcac ctacagccag tgcaacaaag gcggaataca gatttaccgt
    51961 ctcgataccg acagtgcgaa gagcctgccg gtgggctgac tggctattga tcttcttgta
    52021 aggcggatta agtattgcat gggtataacc tcgatcctgt agaccttcgg cggttgccag
    52081 ctcaatataa tccccttcga tgatgcgggg cgtcactctc ctgtacccgg ttaagtgctt
    52141 cgcaaggtga ccacacagct ttttgtcgat ttcgtaggct gttgcctcga cagactcaaa
    52201 gccaaactcg ccaaccagcc aacggtcgag gaaggcgcaa gacagtgcgc ctaaaccagc
    52261 gcctgcgtcc agtagacggc aagttttcat tgtgctcggt ggaaacaagg aggccataaa
    52321 acgggccacg ccagaaggcg tcatgaactg gccgaactct gctttgtgtt tacgcgcggt
    52381 acggggagac agctcgcgac gcacgctgtc tgcgacgtct agcttttgaa gcataactaa
    52441 tcctctttac gtggcatgat gatgccaacg tgcttttgac atcctccaca ttcttcaggc
    52501 gtgaaggatg tcaaatcact ctgtgagctc aatcaaaagt ctcatcattc cactgcgatt
    52561 tgatgacctt tccgacaaac tggcagtttt cgttacactc aatcatgtcg aatcgtgggt
    52621 tgagcggctc caggtacgct ttaccgctct cacgaatgaa tcgtttgaag gtaaattcat
    52681 cgccgttcat tcgggccacg caaaaatcgc catcctcgat ttcccgttca gggtcaacga
    52741 gtatcagcat accttcaggg aagctgggtt taccaccctg cggtgcggtc attgaatgcc
    52801 ccttgacctc cagccagaac gacctgtcac tggcttttgt ggtggtggag atccatttaa
    52861 tcgcatcttt tgcggtgtac gaaatatcat ccattgagaa tgcgccagct tgtacgcagg
    52921 agagtaacgg atattcgtaa gcggggacac gagttaatgg ctcttcaatt gcgagcgcca
    52981 tttctgcaat agattttgcc aggctgggac tgaaatctcc gacctttaca ttcagtatct
    53041 tagcaagctg tgctgcgtgg ctggcattta tggcgttgat gccagccatt agctgattaa
    53101 cagcgctttg ggtaacacct aattcatagg ctaatgattc ctgcgacagg cctaactctt
    53161 tctttttagc gttaaaaata cttttcagac gcttggcgtc ttccagttgc tcaggggtta
    53221 acggtttctt tttcatgttc ataccatatc acctttggtt atatgtaatg aaatatctgc
    53281 ggtgttgaca ttttaataac tgtgcgtaat gatatgggca ttttgaagga gtcagttatg
    53341 gcgatgaaaa aagagaccct ggctgattac gtatacaggt tcggccaaaa gaaagccgct
    53401 aaggactttg gcgtggcgca gagtgcaatc agtaaagcgc ttctcgtcgg acgagaaata
    53461 tacgtcaaaa cctttgatga cggcacagtt gaggcggaag aggttcgtcc gttccctgcg
    53521 ttcgtaagag gcgatgatta atcctggaca cacagcccat cggaggtgtg agtgaacagg
    53581 caaatcaaaa cggttatgcc cgagcagtac tgtgatgctg atcgggactg gatacaggag
    53641 caattgttac agctagaccc gaccacccgg gtaaaaattg cagcagagta cgcgagagtg
    53701 taccaggaag agtgggacaa ggagcctgta tcgttcagga agggcaacag ggcaagacgg
    53761 agcgccaaca cccggttacg ggtgtatgtc cagaagtacg cgagagccag ccgtggctgg
    53821 atgctttcgc cagtagcggt acggaagttt cccccgacac catgatggcg gttagttgcg
    53881 ctagagataa tttcaagtgc aactaatttc ctgaaaggaa gcgaggaggg ggtaaggggg
    53941 tggagtcgtt ggtaaggcct gtatcccttg gcctaggcca gaaggcaggt acataggtta
    54001 ggtagatcac tgtataagcg cgcgatagtt tctggtgaaa tgattatcga gctaactatt
    54061 gagtgataaa ctatgtcggc agcaaccaaa tcggcactga tccgtacact gagcactgta
    54121 ccactgagaa cagaggagcg ttacagcttt ctggctgatg ttgtaacgat ccttgagtca
    54181 caggggatgc atgtggcaag caatgtcaca gtaagaatcg atggcagaaa tttccgtgtt
    54241 gatattctcg caacagcaaa aactggtggg agtgtggcta tcgagatcga tcgtagctct
    54301 ccgcgacccc gctcagtcat gaaattgcgt gaattggcac gtcgtggcac tgaaggcttt
    54361 gtgttgcttc gaatgccaaa gaaactgacc agttatagcg atgctgggat cgatatcatc
    54421 ccggcgaatg gcaagggggc gtcatgctga aaatcacccc aaactttgct caggagcgcg
    54481 gtttaaacca gctgcggcac cagtggaaac agcatcgcac gtacctgatg tatgcaccca
    54541 ctggctctgg caaaactggt cttgcagcat tcgtcactgc cggaatggtt gaacgtggta
    54601 tgcgggtgat gtttgtctgt ccgtacacca tcctgctgaa ccagacagcg gaacgtttca
    54661 ccgaatacgg tttgccgtgg gaagaaatca gttttgtatg gcgtgatcac cctaactatg
    54721 acccgtcacg actgattcag attgccagtg ccgacacgct gatccgtcgt gagtttcctg
    54781 acaacatcga tctgctgatc atcgatgaag cacacatgaa gcgtcgtgcg ttacttgagg
    54841 ttatccggga caaagatatc agggttcttg ggctttccgg caccccgttc gcagcctgga
    54901 tggggaagta ctacgaatgc ctgatcaagc ctacaacgat tcgtgaactt attcagcgtg
    54961 gtgatctgag cgattatgag ttttttgccc cctcaatgcc tgatctggct ggcgtaaaaa
    55021 ccagtaacac cgtatttgga cgggattaca acgaggaaca gctcgcatcc atcatgggga
    55081 gttcggatct ggttggcgat atcgtcagca actggcttga gaatggcgag gatttaccga
    55141 cgatctgctt ctgcgtgaat gtggctcatg cgaactttgt tacccgtgag tttttacagt
    55201 ctggcattgg cgcagaggtg atgacggcag atacccccca tgatgaacga caggacatca
    55261 ttcgccgttt tgaagagggt gcgaccaaaa taattgtcaa cgttggcgta ctggttgcgg
    55321 gatttgacag cgatgttcgc tgcctgatct acgcccgtcc gaccaaatca gaaatccgct
    55381 ggttgcagtg catcgggaga gcgttacgta ctgcgtctgg taaaaaacgc gcattgattt
    55441 tcgaccattc cggcacggtt catcggctgg gcttccccga ggatatcgag tatgacgaac
    55501 ttccgggaaa aaatgacgga atgaaagcgt ctgctggcgg gagtgaggtt aaggctgaga
    55561 aacttcccag ggaatgcccc aaatgccact tcatgaagcc tgctggcgtg catatgtgtc
    55621 ccaaatgtgg atttcgtcct cttggtggtg atgacgtggc gacagatcgc gatcgtaagc
    55681 tttcacgcgt caacaaaggg aaacgcgaat acacccgtga ggaaaaacag cgttggtgga
    55741 gcgagatcaa ggggtatcag aattaccgca acgcgacagg taagccactg agtgacggat
    55801 ggtgtgctca tacctacaag gagaagttcg gggtttggcc taaaggcttc agtaatgcgc
    55861 cgctgcaaac ctcagttgaa gtgtacaact tcatcaagtc aaagaccatt gcctatgcca
    55921 aagggcgcaa gaaagccatg acaggaggcc agcatgcgaa ctagagatgc agccgtgggg
    55981 cactggagcc ggatttttga atattacggc atgcctcctg ttactggtgt gaaacattac
    56041 aacggaccct gcccgatatg tggtgccaga ggtaaatttc gctgtgatga taaggatggt
    56101 tccggttcat ggatttgcgt ctgtggtcac ggggacggga tgaatctgtt gcaacttgcc
    56161 acgggtaagc cctgggtgac gttgtgtgat gaaatcgatc ggctgatcgg aaatacctgg
    56221 aagagggaga aagtcagcca gcctgtaaca gagataagca gaaagcggga gctggtcata
    56281 gataagtttg ccggactccc gtgtctgcgg ggtacaacag gtgaggcgta cctgcagggg
    56341 aggggaatac tccagttacc gaccgaatcc gtgcgttttt gtgaccgtca gatcgccagc
    56401 gggcgcgaat atcaggcaat ttacgccatt gcaacagatg acaaaggttc tctttgctat
    56461 ctgcatcgta cgttgctgga tggtgatcgc aaggcgaatg tagaggcggc taaaaaaatg
    56521 accgcgctac aggagttgcc tggtttgcag catgccaaat cggtggcaat acgcctgtat
    56581 ccggtgtcgt ccactctggg gatagccgag ggtatcgaaa ctgcgctttc atgccgtcaa
    56641 atcttccgct gcaatgtgtg gtcaacaatg aactccggtt ttatggagaa gttcattgcg
    56701 ccacctggcg ttaatcacct gattatcttt gctgacaatg atgcgcacgg cgcaggtctg
    56761 gcggctgcct ttaaatgtgg gcataagaat ctcatgagtt gcaatgacgt tgagaaagtc
    56821 agcattcgct ggcctgactt gccggatttt aacgacatgc tcattcaggg gtgtgaagcc
    56881 cgtgaacatg tgttgacgcg caaattcaaa gcggaggctg cctgatggaa atagagatga
    56941 tcaaggcggc taatggcgta tttgtaccgg cgtatgagcg cgatttaccc cgactggcaa
    57001 aatttaaaaa cggtgagctg tatacactgg aagcaaaact tacccgtaac ccatcttttc
    57061 accggaagat gttcgctttt cttaatttct gctttcagta ctggtgcgct gaacatgctg
    57121 gatacgaatt ctctgatgaa gcgacgcagt ttgatgagtt tcgtaaaaat ctgacaattc
    57181 ttgccgggtt ctatgatgtg gtcacaacca taagaggcga ggtgagatat cgggcaaaaa
    57241 gcctgagtta cgcgaatatg gatcaggatg agtttgaacg ttgctacaac gcaatgatta
    57301 atgccgcgtt aaaacatgtg ttcgggcgct caaacaatcc tgaactgaat aaccgcctgc
    57361 tgtcgttttt ctgaggtgat gatgaagcaa cgtaaaccaa aaaaatgcaa agtgtgcggc
    57421 tcctcgtttg tgccgttccg ctcatatcag aaagtttgct gtggtcagtg cgcactggaa
    57481 ctggtcagaa aagaaaaggc gatagcttca gcaaaagagc aggcagacaa gctgaaagcg
    57541 cgcaggaggg acttacagcc ccgcagttac tggattaagc aggcacaaca ggctgtgaat
    57601 gcttatatca gggagcggga ccgtcatttg ccgtgtgttt catgcgggac gttcgattca
    57661 gcccagtggg atgcaggcca ttaccgtaca acagctgcgg cacctcagct cagatttgat
    57721 gaacgcaata tccataagca atgcgtggtg tgtaaccagt acaaaagcgg aaatctcgtt
    57781 ccgtatcgtg tcgaactgat taaccgcatc gggcaggaag cagtagacga aatcgaatca
    57841 aaccatagtc gccaccgctg gactgtcgaa gagtgcaagg cgatcaaggc agagtatcaa
    57901 cagaaactta aagacctgcg aaacagcaga agtgaggccg catgacgttc accgtaaaaa
    57961 ccattcctga catgcttgtt gaggcatatg gaaatcagac cgaagtggcc cgaatactga
    58021 actgcaatcg tgccacagtc agaaaataca ttggcgataa agaagggaaa agacacgcta
    58081 tcgtcaacgg tgttcttatg gttcaccgcg gatggggtaa agatactgat gcgtgatatc
    58141 cggcaggttc ttgagcgctg gggggcatgg gcggcaaata accatgagga tgttacatgg
    58201 tcgcccattg ctgccggatt taagag
//
LOCUS       DAZKHC020000018        57041 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZKHC020000018 DAZKHC020000000
VERSION     DAZKHC020000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 57041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.018"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            161..2588
                     /gene="pgaA"
                     /locus_tag="ACHOSM_RS06705"
                     /old_locus_tag="pgaptmp_001341"
                     /pseudo
     CDS             161..2588
                     /gene="pgaA"
                     /locus_tag="ACHOSM_RS06705"
                     /old_locus_tag="pgaptmp_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            2597..4615
                     /gene="pgaB"
                     /locus_tag="ACHOSM_RS06710"
                     /old_locus_tag="pgaptmp_001342"
                     /pseudo
     CDS             2597..4615
                     /gene="pgaB"
                     /locus_tag="ACHOSM_RS06710"
                     /old_locus_tag="pgaptmp_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
     gene            4608..5933
                     /gene="pgaC"
                     /locus_tag="ACHOSM_RS06715"
                     /old_locus_tag="pgaptmp_001343"
     CDS             4608..5933
                     /gene="pgaC"
                     /locus_tag="ACHOSM_RS06715"
                     /old_locus_tag="pgaptmp_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HGU4557901.1"
                     /translation="MINRIVSFFILCLVLCIPLCVVYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5935..6348
                     /gene="pgaD"
                     /locus_tag="ACHOSM_RS06720"
                     /old_locus_tag="pgaptmp_001344"
     CDS             5935..6348
                     /gene="pgaD"
                     /locus_tag="ACHOSM_RS06720"
                     /old_locus_tag="pgaptmp_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HGU4557902.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6398..7186)
                     /gene="phoH"
                     /locus_tag="ACHOSM_RS06725"
                     /old_locus_tag="pgaptmp_001345"
     CDS             complement(6398..7186)
                     /gene="phoH"
                     /locus_tag="ACHOSM_RS06725"
                     /old_locus_tag="pgaptmp_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HGU4557903.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7806..9077)
                     /gene="efeB"
                     /locus_tag="ACHOSM_RS06730"
                     /old_locus_tag="pgaptmp_001346"
     CDS             complement(7806..9077)
                     /gene="efeB"
                     /locus_tag="ACHOSM_RS06730"
                     /old_locus_tag="pgaptmp_001346"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HGU4557904.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLS
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9083..10210)
                     /gene="efeO"
                     /locus_tag="ACHOSM_RS06735"
                     /old_locus_tag="pgaptmp_001347"
     CDS             complement(9083..10210)
                     /gene="efeO"
                     /locus_tag="ACHOSM_RS06735"
                     /old_locus_tag="pgaptmp_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HGU4557905.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVAVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNLKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10268..11098)
                     /gene="efeU"
                     /locus_tag="ACHOSM_RS06740"
                     /old_locus_tag="pgaptmp_001348"
     CDS             complement(10268..11098)
                     /gene="efeU"
                     /locus_tag="ACHOSM_RS06740"
                     /old_locus_tag="pgaptmp_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HGU4557906.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            11134..11436
                     /locus_tag="ACHOSM_RS06745"
                     /old_locus_tag="pgaptmp_001349"
     CDS             11134..11436
                     /locus_tag="ACHOSM_RS06745"
                     /old_locus_tag="pgaptmp_001349"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557907.1"
                     /translation="MQRKVNRSDSDYHYGKEKHKRNVYKMMLECKQREVMPGDINDYS
                     RVERWDCLQRTMCSCRMPRKRHIQPTKLSRLNASELPCKTDKRVYQAISSIRDYQI"
     gene            complement(11767..13275)
                     /gene="putP"
                     /locus_tag="ACHOSM_RS06750"
                     /old_locus_tag="pgaptmp_001350"
     CDS             complement(11767..13275)
                     /gene="putP"
                     /locus_tag="ACHOSM_RS06750"
                     /old_locus_tag="pgaptmp_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HGU4557908.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            complement(13434..13643)
                     /locus_tag="ACHOSM_RS06755"
                     /old_locus_tag="pgaptmp_001351"
     CDS             complement(13434..13643)
                     /locus_tag="ACHOSM_RS06755"
                     /old_locus_tag="pgaptmp_001351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557909.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            13698..17660
                     /gene="putA"
                     /locus_tag="ACHOSM_RS06760"
                     /old_locus_tag="pgaptmp_001352"
     CDS             13698..17660
                     /gene="putA"
                     /locus_tag="ACHOSM_RS06760"
                     /old_locus_tag="pgaptmp_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4557910.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGRHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVAMLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSKAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGLTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17700..18338)
                     /gene="rutR"
                     /locus_tag="ACHOSM_RS06765"
                     /old_locus_tag="pgaptmp_001353"
     CDS             complement(17700..18338)
                     /gene="rutR"
                     /locus_tag="ACHOSM_RS06765"
                     /old_locus_tag="pgaptmp_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HGU4557911.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            18626..19717
                     /gene="rutA"
                     /locus_tag="ACHOSM_RS06770"
                     /old_locus_tag="pgaptmp_001354"
     CDS             18626..19717
                     /gene="rutA"
                     /locus_tag="ACHOSM_RS06770"
                     /old_locus_tag="pgaptmp_001354"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HGU4557912.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            19717..20409
                     /gene="rutB"
                     /locus_tag="ACHOSM_RS06775"
                     /old_locus_tag="pgaptmp_001355"
     CDS             19717..20409
                     /gene="rutB"
                     /locus_tag="ACHOSM_RS06775"
                     /old_locus_tag="pgaptmp_001355"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HGU4557913.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFAHIA"
     gene            20421..20807
                     /gene="rutC"
                     /locus_tag="ACHOSM_RS06780"
                     /old_locus_tag="pgaptmp_001356"
     CDS             20421..20807
                     /gene="rutC"
                     /locus_tag="ACHOSM_RS06780"
                     /old_locus_tag="pgaptmp_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HGU4557914.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            20815..21615
                     /gene="rutD"
                     /locus_tag="ACHOSM_RS06785"
                     /old_locus_tag="pgaptmp_001357"
     CDS             20815..21615
                     /gene="rutD"
                     /locus_tag="ACHOSM_RS06785"
                     /old_locus_tag="pgaptmp_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HGU4557915.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVIGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHAERIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            21625..22215
                     /gene="rutE"
                     /locus_tag="ACHOSM_RS06790"
                     /old_locus_tag="pgaptmp_001358"
     CDS             21625..22215
                     /gene="rutE"
                     /locus_tag="ACHOSM_RS06790"
                     /old_locus_tag="pgaptmp_001358"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HGU4557916.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            22226..22720
                     /gene="rutF"
                     /locus_tag="ACHOSM_RS06795"
                     /old_locus_tag="pgaptmp_001359"
     CDS             22226..22720
                     /gene="rutF"
                     /locus_tag="ACHOSM_RS06795"
                     /old_locus_tag="pgaptmp_001359"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HGU4557917.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            22741..24069
                     /gene="rutG"
                     /locus_tag="ACHOSM_RS06800"
                     /old_locus_tag="pgaptmp_001360"
     CDS             22741..24069
                     /gene="rutG"
                     /locus_tag="ACHOSM_RS06800"
                     /old_locus_tag="pgaptmp_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HGU4557918.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAPVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(24152..24325)
                     /gene="ymdF"
                     /locus_tag="ACHOSM_RS06805"
                     /old_locus_tag="pgaptmp_001361"
     CDS             complement(24152..24325)
                     /gene="ymdF"
                     /locus_tag="ACHOSM_RS06805"
                     /old_locus_tag="pgaptmp_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGU4557919.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            25094..25167
                     /locus_tag="ACHOSM_RS06810"
                     /old_locus_tag="pgaptmp_001362"
                     /pseudo
     CDS             25094..25167
                     /locus_tag="ACHOSM_RS06810"
                     /old_locus_tag="pgaptmp_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813257.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            complement(25257..33638)
                     /locus_tag="ACHOSM_RS06815"
                     /old_locus_tag="pgaptmp_001363"
     CDS             complement(25257..33638)
                     /locus_tag="ACHOSM_RS06815"
                     /old_locus_tag="pgaptmp_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HGU4557920.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKATGKAVSDGAKATDEWLTGK
                     MSPGAVRALNTPMTEGYNDSAVWVAKGVNLIGALVPDMVAGGVARKVGDVTLRKMLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTVSAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATAQGAINAAQGGYSRYQENTALRETA
                     GMDVSPWEGVADATIEGAALGAAMGAPFGAVAGYRGRRQAAEETAMRDAETVQQDDAA
                     PQPESVDPVAQQRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQ
                     TKRQAVMNELKAKPRSELLEEYRRLSQKEGRTETEEQQFQAIREVIRPQQEVTPEAQS
                     QPENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMG
                     SDVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADRERIADADPME
                     QPAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQELTERPRL
                     PAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAPENAAGR
                     QGETLEGEMVRRGLPSPDAQNATAPVREGLPAPDITHNVRMPQPDQLPRTVRNSLPEL
                     AQQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGEEIKGA
                     AKHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLASEGADADTLAMIALYRSDI
                     PAKTKHNTAGWGESVKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLP
                     PSQMDKASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGTNLFFSESRDELLTKAK
                     VWFAELEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSAS
                     DARKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFR
                     GVQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAA
                     HYEPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSNDGKITSGGDFMTEAQRVRR
                     IFKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSVLLDEVRSKPYWSTDVEMAA
                     RAFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIREAFDHLFRT
                     LKTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDS
                     DLKVRVVKSQKEAEALAGELFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHE
                     IIHHAMEHVVTPAEYQTIIKTVLKTRDSDNVTIREAWRKVDASYGKESPEVQAGEFLA
                     HMAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYT
                     PREDGAGASSQYSRSGKRDPFKVPEGEGERYRDDLARMMKSLRTTDLTVNIGRTPPVL
                     RHLGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVM
                     LLDAVDKNGDPVVSAVHMKAVRSRLEINKVASVYGTENGKKLKSMEMTGLTLYRREKL
                     SRDNLLHRGLQLPKGEHSYRGSADKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQM
                     QDKFQVLKAVQENIRKTGGKVDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLA
                     DYKIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQA
                     QYDRLAGIIDDMLARRRELIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRT
                     GKGFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDK
                     DYWQVFTDDRPDTMRTIAERKDQETGETIREVVERPVPMAMMADRYFTTKKNGKTYYI
                     KLHDPRLMRAMKSMGPETSNAFVRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVM
                     NLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEF
                     VEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENA
                     LRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLV
                     RTLGHLNGDGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDK
                     VPSHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAG
                     NVVGGVLGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPF
                     GTPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRSGAVDITPESLKYWVDYISGGTG
                     RFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYVDQQKMYDRMTEVAQYHAELK
                     SLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKS
                     VERDMKIAVDRFNREYNKKVGVD"
     gene            complement(33708..34973)
                     /locus_tag="ACHOSM_RS06820"
                     /old_locus_tag="pgaptmp_001364"
     CDS             complement(33708..34973)
                     /locus_tag="ACHOSM_RS06820"
                     /old_locus_tag="pgaptmp_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557921.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLARQQKNNERDFE
                     FAQSQFEHNKNVDQRNFDYRAKVDDRNYALKEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMIAHSQPLMEALGKAIEAGDQEAATRLFGQLPKGHPLILMSNEGYAAKA
                     GQAVINLQKIFGDKPDMAIDSLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRI
                     QKARGEQARDESASSASQFIRGLKQNYAQ"
     gene            complement(34984..35235)
                     /locus_tag="ACHOSM_RS06825"
                     /old_locus_tag="pgaptmp_001365"
     CDS             complement(34984..35235)
                     /locus_tag="ACHOSM_RS06825"
                     /old_locus_tag="pgaptmp_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HGU4557922.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRREEIEAANKQMAAQQKAQ
                     NKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            complement(35245..35691)
                     /locus_tag="ACHOSM_RS06830"
                     /old_locus_tag="pgaptmp_001366"
     CDS             complement(35245..35691)
                     /locus_tag="ACHOSM_RS06830"
                     /old_locus_tag="pgaptmp_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557923.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVNRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            complement(35694..36350)
                     /locus_tag="ACHOSM_RS06835"
                     /old_locus_tag="pgaptmp_001367"
     CDS             complement(35694..36350)
                     /locus_tag="ACHOSM_RS06835"
                     /old_locus_tag="pgaptmp_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557924.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARRELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            complement(36444..36845)
                     /locus_tag="ACHOSM_RS06840"
                     /old_locus_tag="pgaptmp_001368"
     CDS             complement(36444..36845)
                     /locus_tag="ACHOSM_RS06840"
                     /old_locus_tag="pgaptmp_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557925.1"
                     /translation="MAPVSGERMNDKILWYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTHLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            complement(36902..37042)
                     /locus_tag="ACHOSM_RS06845"
                     /old_locus_tag="pgaptmp_001369"
     CDS             complement(36902..37042)
                     /locus_tag="ACHOSM_RS06845"
                     /old_locus_tag="pgaptmp_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4557926.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            complement(37275..38009)
                     /locus_tag="ACHOSM_RS06850"
                     /old_locus_tag="pgaptmp_001370"
     CDS             complement(37275..38009)
                     /locus_tag="ACHOSM_RS06850"
                     /old_locus_tag="pgaptmp_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4557927.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGEHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
     gene            complement(38100..38717)
                     /locus_tag="ACHOSM_RS06855"
                     /old_locus_tag="pgaptmp_001371"
     CDS             complement(38100..38717)
                     /locus_tag="ACHOSM_RS06855"
                     /old_locus_tag="pgaptmp_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557928.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            complement(38723..39001)
                     /locus_tag="ACHOSM_RS06860"
                     /old_locus_tag="pgaptmp_001372"
     CDS             complement(38723..39001)
                     /locus_tag="ACHOSM_RS06860"
                     /old_locus_tag="pgaptmp_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HGU4557929.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINDNTVTWSNLEQGMGSYIYTFWG"
     gene            complement(39016..40284)
                     /locus_tag="ACHOSM_RS06865"
                     /old_locus_tag="pgaptmp_001373"
     CDS             complement(39016..40284)
                     /locus_tag="ACHOSM_RS06865"
                     /old_locus_tag="pgaptmp_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4557930.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            complement(40281..41906)
                     /locus_tag="ACHOSM_RS06870"
                     /old_locus_tag="pgaptmp_001374"
     CDS             complement(40281..41906)
                     /locus_tag="ACHOSM_RS06870"
                     /old_locus_tag="pgaptmp_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557931.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPIAQDPHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPASSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVINFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            complement(42140..42652)
                     /locus_tag="ACHOSM_RS06875"
                     /old_locus_tag="pgaptmp_001375"
     CDS             complement(42140..42652)
                     /locus_tag="ACHOSM_RS06875"
                     /old_locus_tag="pgaptmp_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557932.1"
                     /translation="MGITSGWVGSSAKSETGEQWMGAAGTKLGLDKPFMMSQMVGRAM
                     GCKIETAYYKWNSSDQVENWGAVGADWPLEEKSKGTITNAENCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLSGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVTQDLKIS"
     gene            complement(42739..44976)
                     /locus_tag="ACHOSM_RS06880"
                     /old_locus_tag="pgaptmp_001376"
     CDS             complement(42739..44976)
                     /locus_tag="ACHOSM_RS06880"
                     /old_locus_tag="pgaptmp_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HGU4557933.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLANIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAVTAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPGLKGDTGERGPKGDQGDMGPKGEKG
                     DPGGPAGPQGPKGERGEAGPQGPMGARGERGETGPRGEPGPAGPRGERGETGPQGPRG
                     EPGPAGSAANVADATTAQKGIVQLSSATDSDDEAKAATPKAVKAAMDVANEAKTKAEE
                     AAAGGGVPGPKGDKGDSGPAGPAGPRGVQGPKGDPGPQGPKGDTGAAGAKGEKGATGA
                     TGPQGPKGDTGAAGPAGPQGPKGDTGAAGPAGPQGPKGDTGAAGPAGAQGPKGDKGDP
                     GVAGPAGPAGAPGPKGDKGDPGVAGPAGPEGPQGPKGDTGAPGQGTELLTTANTWTQA
                     QTFNGGINGNLTVNGNGSFNDVQIRSDKRNKRNLVKLDNALDRLEALTGYLYEIQYSA
                     DGWQTSVGLIAQDAQKALPELVTEDADVISGEKRLRLNYNGIIALLVEGFKTLRHEIK
                     ELREK"
     gene            complement(44973..45623)
                     /locus_tag="ACHOSM_RS06885"
                     /old_locus_tag="pgaptmp_001377"
     CDS             complement(44973..45623)
                     /locus_tag="ACHOSM_RS06885"
                     /old_locus_tag="pgaptmp_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HGU4557934.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTVKG
                     IPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADRALSARKKVFNGGGV"
     gene            complement(45623..46186)
                     /locus_tag="ACHOSM_RS06890"
                     /old_locus_tag="pgaptmp_001378"
     CDS             complement(45623..46186)
                     /locus_tag="ACHOSM_RS06890"
                     /old_locus_tag="pgaptmp_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557935.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFF"
     gene            complement(46170..46631)
                     /locus_tag="ACHOSM_RS06895"
                     /old_locus_tag="pgaptmp_001379"
     CDS             complement(46170..46631)
                     /locus_tag="ACHOSM_RS06895"
                     /old_locus_tag="pgaptmp_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557936.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            complement(46681..47070)
                     /locus_tag="ACHOSM_RS06900"
                     /old_locus_tag="pgaptmp_001380"
     CDS             complement(46681..47070)
                     /locus_tag="ACHOSM_RS06900"
                     /old_locus_tag="pgaptmp_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557937.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            complement(47126..48340)
                     /locus_tag="ACHOSM_RS06905"
                     /old_locus_tag="pgaptmp_001381"
     CDS             complement(47126..48340)
                     /locus_tag="ACHOSM_RS06905"
                     /old_locus_tag="pgaptmp_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HGU4557938.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            complement(48364..49371)
                     /locus_tag="ACHOSM_RS06910"
                     /old_locus_tag="pgaptmp_001382"
     CDS             complement(48364..49371)
                     /locus_tag="ACHOSM_RS06910"
                     /old_locus_tag="pgaptmp_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557939.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSAQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNKADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            complement(49529..51673)
                     /locus_tag="ACHOSM_RS06915"
                     /old_locus_tag="pgaptmp_001383"
     CDS             complement(49529..51673)
                     /locus_tag="ACHOSM_RS06915"
                     /old_locus_tag="pgaptmp_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGU4557940.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRQQNEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDNDLMEQI
                     ERPDGIIKLNPVRKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            complement(51673..53379)
                     /locus_tag="ACHOSM_RS06920"
                     /old_locus_tag="pgaptmp_001384"
     CDS             complement(51673..53379)
                     /locus_tag="ACHOSM_RS06920"
                     /old_locus_tag="pgaptmp_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4557941.1"
                     /translation="MTFRKNEPRCDEPSEMTEAEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRSMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYINKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            complement(53360..54166)
                     /locus_tag="ACHOSM_RS06925"
                     /old_locus_tag="pgaptmp_001385"
     CDS             complement(53360..54166)
                     /locus_tag="ACHOSM_RS06925"
                     /old_locus_tag="pgaptmp_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4557942.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPCNDAGSGDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVVTPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            complement(54459..55010)
                     /locus_tag="ACHOSM_RS06930"
                     /old_locus_tag="pgaptmp_001386"
     CDS             complement(54459..55010)
                     /locus_tag="ACHOSM_RS06930"
                     /old_locus_tag="pgaptmp_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4557943.1"
                     /translation="MNYPTIVNGIDFRDLVFVTGNEPVTDTRKVAVAFGKEHKDVLRK
                     TRAVVQQCSKEFAERNFALCYENNELQNGKPQPIYRMTKDGWTMLVFGFTGKAAMTFK
                     EAYIQAFNWMAELIQQGLANLEAERNAVMLEYMKEKDVASMSGRLLNRWGRVKKPQLL
                     ARLDRLEQQGQIALPGFDKGISA"
     gene            complement(55213..55650)
                     /locus_tag="ACHOSM_RS06935"
                     /old_locus_tag="pgaptmp_001387"
     CDS             complement(55213..55650)
                     /locus_tag="ACHOSM_RS06935"
                     /old_locus_tag="pgaptmp_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4557944.1"
                     /translation="MNRMLSAFTVILLVVCGALSLGLNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(55653..55802)
                     /locus_tag="ACHOSM_RS06940"
                     /old_locus_tag="pgaptmp_001388"
     CDS             complement(55653..55802)
                     /locus_tag="ACHOSM_RS06940"
                     /old_locus_tag="pgaptmp_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4557945.1"
                     /translation="MEWWLMISDKLITLAKGLCVIVGISFSLMLVVLFLSINWMALTS
                     AGLVG"
     gene            complement(55802..56371)
                     /locus_tag="ACHOSM_RS06945"
                     /old_locus_tag="pgaptmp_001389"
     CDS             complement(55802..56371)
                     /locus_tag="ACHOSM_RS06945"
                     /old_locus_tag="pgaptmp_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HGU4557946.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRDKVIQYQEEC
                     DDVLYEYWTKGGVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLINEANLLIDFVLADTGKGKITKAD"
     gene            complement(56645..>57041)
                     /locus_tag="ACHOSM_RS06950"
                     /old_locus_tag="pgaptmp_001390"
     CDS             complement(56645..>57041)
                     /locus_tag="ACHOSM_RS06950"
                     /old_locus_tag="pgaptmp_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4557947.1"
                     /translation="GIWTICRGATQVDGKLVVPGMKLSKEKCDQVNAIERDKALAWVA
                     KNIRVPLTEPQKAGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGR
                     DCRIRSNNCYGQVSRRDQESALACWGIDR"
BASE COUNT        13254 a        15849 c        13535 g        14403 t
ORIGIN      
        1 atatggacac tctgctcatc atttcttctt ctcatcatca acaattcacg tctctcttcc
       61 gcgtttaata acggattatg aggtgcaaaa atatctttct tttcagttac ctgtaattag
      121 atacagagag agattttggc aatacatgga gtaatacagg atgtattcaa gtagcagaaa
      181 aaggtgcccg aaaaccaaat gggctttgaa acttcttact gccgcatttt tagcagcgag
      241 tcccgcggcg aagagtgctg ttaataacgc ctatgatgca ttgattattg aagctcgcaa
      301 gggtaatact cagccagctt tgtcatggtt tgcactaaaa tcagcactca gcaataacca
      361 aattgctgac tggttacaga ttgccttatg ggccgggcaa gataaacagg ttattaccgt
      421 ttacaaccgc taccgtcatc agcaattacc agcgcgtggt tatgcagctg tcgccgtcgc
      481 ttatcgtaac ctgcaacaat ggcaaaactc gcttacactg tggcaaaagg cgctctctct
      541 ggagccgcaa aataaggatt atcaacgggg acaaatttta accctggcag atgctggtca
      601 ctatgatact gcgctggtta aacttaagca gcttaactct ggagcaccgg acaaagccaa
      661 tttactcgca gaagcctata tctataaact ggcggggcgt catcaggatg aattacgggc
      721 gatgacagag tcattacctg aaaatgcatc tacgcaacaa tatcccacag aatacgtgca
      781 ggcattacgt aataatcaac ttgctgccgc gattgacgat gccaatttaa cgccagatat
      841 tcgcgctgat attcatgccg aactggtcag actgtcgttt atgcctacgc gcagtgaaag
      901 tgaacgttat gccattgccg atcgcgccct cgcccaatac gctgcattag aaattctgtg
      961 gcacgataac ccagaccgca ctgcccagta ccagcgtatt caggttgatc atcttggcgc
     1021 gttattaact cgcgatcgtt ataaagacgt tatttctcac tatcagcgat taaaaaagac
     1081 ggggcaaatt attccgccct gggggcaata ttgggttgca tcggcttatc tcaaagatca
     1141 tcagccgaaa aaagcacagt caataatgac cgagctcttt tatcacaagg agaccattgc
     1201 cccggattta tccgatgaag aacttgcgga tctcttttac agccacctgg agagtgaaaa
     1261 ttatccgggc gcgctaactg tcacccaaca taccattaat acttcgccgc ctttccttcg
     1321 gttaatgggc acgcctacga gcatcccgaa tgatacctgg ttacaggggc attcgtttct
     1381 ctcaaccgta gcaaaatata gtaatgatct tcctcaggct gaaatgacag ccagagagct
     1441 tgcttataac gcaccaggaa atcagggact gcgcattgat tacgcgagtg tgttacaagc
     1501 ccgcggttgg cctcgtgcag cagaaaatga attaaaaaaa gcagaagtga tcgagccacg
     1561 taatattaat ctggaggttg aacaagcctg gacagcatta acgttacaag aatggcagca
     1621 ggcagctgtc ttaacgcacg atgttgtcga acgtgaaccg caagatcccg gcgttgtacg
     1681 tttaaaacgt gcggttgatg tacataatct tgcagagctt cgtatagctg gttcaacagg
     1741 aattgatgcc gaaggcccgg atagtggtaa acatgatgtc gacttaacca ccatcgttta
     1801 ttcaccaccg ctgaaggata actggcgcgg ttttgctgga ttcggttatg ccgatggaca
     1861 atttagcgaa ggaaaaggga ttgttcgcga ctggctggcg ggtgttgagt ggcggtcacg
     1921 taatatctgg ctcgaggcgg agtacgctgt acgctttttc aatcatgagc ataaacccgg
     1981 cgcgcgcctg tctggctggt atgattttaa tgataactgg cgtattggtt cgcaactgga
     2041 acgcctctct caccgcgttc cattacgggc aatgaaaaat ggtgttacag gcaacagtgc
     2101 tcaggcttat gttcgctggt atcaaaatga gcggcgtaag tacggtgtct cctgggcttt
     2161 cactgatttt tccgacagta accagcgtca tgaagtctca attgagggtc aggaacgcat
     2221 ctggtcttca ccatatttga ttgtctattt cctacccagt ctgtattacg aacaaaatac
     2281 agaacacgat accccatact acaaccctat aaaaacgttc gatattgttc cggcatttga
     2341 ggcaagtcat ttgttatggc gaagctatga aaatagctgg gagcaaatct tcagcgcagg
     2401 tgttggtgcc tcctggcaaa aacattatgg cacggaagtc gtcacccacc caactcggct
     2461 acgggcaacg cattagctgg aatgacgtga ttgatgctgg cgcaacgcta cgctgggaaa
     2521 aacgacctta tgacggtgac agagaacaca acttatacgt tgaattcgat atgacattca
     2581 gattttaagg ataaatatgt tacgtaatgg aaataaatat ctcctgatgc tggtgagtat
     2641 aattatgctc accgcgtgca tcagccagtc aagaacatca tttataccgc cacaggatcg
     2701 cgaatcttta ctcgccgagc aaccgtggcc gcataatggt tttgtggcga tttcatggca
     2761 taacgttgaa gacgaagctg ccgaccagcg ttttatgtca gtgcggacat cagcactgcg
     2821 tgaacaattt gcctagctgc gcgagaacgg ttatcaaccg gtcagtattg ctcaaattcg
     2881 tgaagcacat cgaggaggaa aaccgctacc ggaaaaagca gtagtgctga cttttgatga
     2941 cggctaccag agtttttata cccgcgtctt cccaattctt caggccttcc agtggcctgc
     3001 tgtatgggcc cccgtcggca gttgggtcga tacgccagcg gatgaacaag taaaatttgg
     3061 cgatgagatg gtcgatcgag aatattttgc cacgtggcaa caagtgcgag aagttgcgcg
     3121 ttcccggctc gttgaggtcg cttctcatac atggaattct cactacggta ttcaggctaa
     3181 ttccaccggc agcttaatgc ctgtatatgt aaatcgtgca tattttactg accacgcacg
     3241 gtatgaaacc gcagcagaat accgggaaag aattcgtctg gatgctgtaa aaatgacgga
     3301 atacctgcgt acaaaggctg aggtaaatcc acacgttttt gtttggcctt atggcgaagc
     3361 gaatggcata gcgatagagg aattaaaaaa actcggttat gacatgtttt tcacccttga
     3421 atcaggtttg gcaaatgcgt cacaattgga ttccattccg cgagtattaa tcgccaataa
     3481 tccctcatta aaagagtttg cccagcaaat tattaccgta caggaaaaat caccacaacg
     3541 gataatgcat atcgatcttg attacgttta tgacgaaaac cgccagcaaa tggatcgcaa
     3601 tattgatgtg ctaattcagc gggtgaaaga tatgcaaata tcaaccgtgt atttgcaggc
     3661 atttgctgat cccgatggtg atgggctggt caaagaggtc tggtttccaa atcgtttgct
     3721 accaatgaaa gcagatattt ttagtcgggt tgcctggcaa ttacgtaccc gctcaggtgt
     3781 aaacatctat gcgtggatgc cggtattaag ctgggattta gatcccacat taacgcgagt
     3841 aaaatactta ccaacagggg agaaaaaagc acaaattcat cctgaacaat atcaccgtct
     3901 ctctcctttc gatgacagag tcagagcaca agttggcatg ttatatgaag atcttgccgg
     3961 acatgctgct tttgatggca tattgttcca cgatgatgct ttgctttcag attatgaaga
     4021 tgccagtgca ccggctatca cggcttatca gcaagcaggc tttagcggga gtctgagcga
     4081 aattcgacaa aacccggagc aatttaaaca gtgggcccgc tttaaaagtc gtgcgttaac
     4141 tgacttcact ttagaactta gtgcgcgcgt aaaagccatt cgcggtccac atattaaaac
     4201 tgcacgaaat atttttgcac ttccggtaat acaacctgaa agtgaagcct ggtttgcaca
     4261 gaattatgct gatttcctaa aaagctatga ctggaccgct attatggcta tgccttatat
     4321 ggaaggtgtc gcagaaaaat cggctgacca atggttaata caattgacca atcaaattaa
     4381 aaacatccct caggctaaag acaaatctat tttagaatta caggcacaaa actggcagaa
     4441 aaatggtcag catcaggcta tttcttcgca acaactcgct cactggatga gcctattaca
     4501 actgaatgga gtgaaaaact atggttatta tcccgacaat tttctgcata accaacctga
     4561 aatagacctt attcgtcctg agttttcaac agcctggtat ccgaaaaatg attaatcgca
     4621 tcgtatcgtt ttttatatta tgtctggtgt tatgcatacc cctatgcgta gtgtactttc
     4681 actctggtga actgatgatg aggttcgttt tcttctggcc gttttttatg tccattatgt
     4741 ggattgttgg cggcgtctat ttctgggtct atcgtgaacg ccactggccg tggggagaaa
     4801 acgcaccagc tccccagttg aaagataatc cgtctatctc cattatcatt ccctgtttta
     4861 atgaggagaa aaacgttgag gaaaccatac acgccgcttt agcacagcgt tatgagaaca
     4921 ttgaagttat tgccgtaaat gacggttcaa cagataaaac ccgtgccatc ctggatcgca
     4981 tggctgcaca aattccccat ttgcgggtca ttcatctggc gcaaaaccag gggaaagcca
     5041 ttgcgcttaa aaccggagct gccgcggcga aaagtgaata tctggtgtgc attgatggcg
     5101 atgcgttatt agaccgcgat gcggcggcat atattgtgga accgatgttg tacaacccgc
     5161 gtgtgggtgc cgtaaccggt aatcctcgta ttcgaacacg ttctaccctg gtgggtaaaa
     5221 ttcaggttgg cgagtattcc tcaattattg gtttgatcaa gcgaacccag cgtatctatg
     5281 gaaacgtatt taccgtttcc ggtgttattg ccgcatttcg tcgcagcgcc ctggcagaag
     5341 tgggttactg gagtgacgat atgatcaccg aagatattga tattagctgg aagctgcagt
     5401 tgaatcagtg gacgattttt tacgagccac gggcgctgtg ctggatatta atgcctgaaa
     5461 cgttaaaagg gctgtggaaa cagcgcctgc gctgggctca gggcggtgca gaagtattcc
     5521 tcaaaaatat gacaaggttg tggcgcaaag aaaactttcg aatgtggccg ctgttttttg
     5581 aatactgcct gacgacaata tgggccttca cctgcctggt cggtttcatt atttacgcag
     5641 tccaacttgc cggtgtaccg ttaaatattg aattgacaca tatcgctgcg acacatactg
     5701 ccggaatatt attgtgtacg ttatgtttac tgcaatttat tgtcagcctg atgatcgaga
     5761 atcgctatga gcataatctg acttcatcgc ttttctggat tatttggttc ccggttattt
     5821 tctggatgct gagcctggca acgacattgg tatcatttac acgagtcatg ttgatgccta
     5881 aaaagcaacg cgcccgttgg gtaagtcctg atcgcgggat tctgagaggt taatatgaac
     5941 aatttaatta ttacgacccg acaatcacca gtacgtttac tggttgatta tgttgccaca
     6001 accatcttgt ggacattatt tgctttgttc atattcttat tcgccatgga tctgctgacg
     6061 ggttattact ggcaaagcga ggccagaagc cgacttcagt tctatttttt gctggcagtg
     6121 gcgaatgccg tcgtgttaat tgtctgggcg ctgtacaata agctgcgttt tcaaaaacag
     6181 cagcatcatg cagcctacca atatacgccg caagaatatg cagagagctt agcaatacct
     6241 gatgagctct atcagcaact acaaaaaagc cacaggatga gcgtacactt caccagccag
     6301 gggcaaataa aaatggttgt ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca
     6361 aagcccggtt cgcccgggct ctgcaccgat aatacactta actgtatgca tgcagcgtac
     6421 gttggcaaag tgccgaacgt acgcaatcct cttttccgaa ccggacgatc ccaaccattt
     6481 catcttcttc gaaacgttcc agcgcgtcac ttaatccgga gcagacgccg cgaggcaaat
     6541 cgcattgcgt gatatcaccg ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa
     6601 acattttcat ttgcgcggca gtcacattct gcgcctcgtc aagaatgacg actgcatttt
     6661 caaaggtacg tccacgcata taggcgaacg gcgcgatttc caccttccct atttccggtc
     6721 gcaggcagta ctgcataaag gaagccccta agcgccggac cagcaagtcg tagaccgggc
     6781 gaaaataggg agcaaacttt tctgcgatat ctccaggtaa gaagccaaga tcttcatcgg
     6841 cttgcagaac tggacgggtg acgataatcc tgtcgacatc cttatgtatc agggcctctg
     6901 ccgcttttgc tgcgctgatc caggtttttc cgcacccggc ttcgcccgtg gcgaatatca
     6961 gctgcttact ctcaatagcc ttcagatagt gcaattgcgc ttcatttcgc gcgaggatgg
     7021 gcgaagtatc gcgactgtcg cgggccatac caatggcttc tacgccgccc atctgcacaa
     7081 gcgaggtgac cgattcttct tcacgctgct tatggctgcg cgaatcccgt ctcagcacac
     7141 gttttgcctc gcgacgagct ttgatcactg ctttttgtct tcccatggag agcaccttga
     7201 gttgtttgta ttcatcacac gcgccgttgg cagcgcgatt atgcgcacga acatcagagg
     7261 gttggcttcc ttgtaagcca tagtttgctt ttggataaaa tgccgaaaaa cggctacgcg
     7321 caccgtttac ggcgtcggta acacatgaaa agaaaggatg aggttgaaaa tgcaaagtga
     7381 cgagatgact accggaggag aaaactccgc gagtggtggc gcgttgatta tctaaaacat
     7441 gtccagtaca ggacgttacc atccgcgatc tccatagtga ctgactatca ctgccgggaa
     7501 cttacgctgc tacttaataa gtacaacaga tctcgcattt attgcaacaa tatatttact
     7561 tatatttaac tataaaacac catttcagtg acattagttt ctactggaaa gatgacagag
     7621 tgatgacaga tatgaaaaaa gctgtgtgct ttcagcagga ttcgcatcag gcatctgcgc
     7681 acgaacgccg tatgcggcgt gaacgcctta tccgcctacc gaacccgcgc cgattgtagg
     7741 cataagaaga cgcgcaagca tcgcatcagg catctgcgca agaacgcctt atccgtctaa
     7801 aaacattaaa cccgcaataa cgcgcttccg agataatcgt tcgcgtcctt cactcccggc
     7861 agcgcaaaaa aataaccgcc gccgataggt ttaacgtatt cctccagcgc ttcgccattg
     7921 agcctttttt gtactgtcag gaagcctttt tccagatcgt gttggtaaca gacaaacagt
     7981 agccccatat ccagttgccc ggagttggtg acgcccagtg aataactgta gccacgacgc
     8041 agcatcaggc tggactcact ctccgccgtg cggggattcg ccagccggat atggctgtcc
     8101 agcgcgatca ccttcccttc cgggtcgctg gcgtaatcag gcacatcatg ctcatgctgc
     8161 attcccagcg gcgcaccggt ttgcttatca cggccaaaaa tcgtctgctg ttctttcagc
     8221 ggcgttctgt cccaaaattc cactcgaaac tgaatcaagc gtactgcctg atagctgcca
     8281 ccgattgtcc acgcaggctc ctgctgatct gccgttaccc acaccacttt ttgcatcaac
     8341 ttatcattct ggctatcggg attggcagtg ccgtctttga aacccagcaa attaatcggc
     8401 gtctctttgc ctttactacg cgccgcgtga tcggaaataa acccttcccg cttccagcgc
     8461 acactgagca aatccggcgt gtgtttgatg atatcgcgca gcgcatggat aaccgtgtcc
     8521 tgggtgttgg cgcaaatctg tagcaacaca tcaccatgac ataacgccgc atccagcgag
     8581 tcgttgggga aacgcgtcat cttctgcagc ttttttggca tctgtggcgc aaggccaaag
     8641 cgctcatcaa acaatgagtg acccaccgat aacgtgatgg tgagattatc gggcgcaatg
     8701 tagccgccaa gaatgccgga atcgagtggt ggcaggcgcg gatttggcgt ttctggtgct
     8761 gctccgccct gactcagaaa agcaaaacgc tgagtcaaca agcgaaacaa ccgctcaaga
     8821 tcggctttat cgctggcaag cacatcaaac gccaccagca tcattgcggc ctgttgtggc
     8881 gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt tctcattgcg tgcatccggt
     8941 gaaagcgtgc ccggtgcact ttgcgttttt tgtgcatgag cgaccggaca acttcccgcc
     9001 agcgccagcg caccaatccc tttcagtaaa cgtcggcgtg acggttcatt cacgccgttt
     9061 tcatctttat actgcataac gcttaatcca gtcccagcac accgcgaagt tgcgccagat
     9121 cttccgccag cgcagtaatc ggtcctttca gtgcattccg gtcggcatcg gtcaatttgt
     9181 cgtaggtttc aaaaccgtct ttagtacggt atttcgccag aatggtatcg acctttttaa
     9241 agttggcatc gacttttgcc agcagttccg ggttggcttt ttgcagttgt ggacgcagca
     9301 aatcgacaat tttctgcgag ccttcaacgt tagcctggaa atcccacaga tcggtgtggc
     9361 tgtagcgatc ttcttcaccg ctaattttgc tggctgccac ttcctcaatc agtccggctg
     9421 cgccgccaac cacttttgaa ggtgggaaag ccagttcact gatgcgtttt tgcaaatcga
     9481 ccacatctgt ataaagctgc tcagcgtact gatccatccc tttggtggtg ttgtcgccaa
     9541 acaatgcttt ttccagacgg tggaaaccag tgaattttgg gtcggcggct ttttgctcgt
     9601 aatcatcttc acgggcgtca atgctgccat ccagatcgga gaacagttca gcaatcggtt
     9661 caatgcgctc atagtgctgg cgcgtcggcg catacagtgc tttcgctttt tcgatatcgc
     9721 ctgctttaat cgcgtcggta aaggctttgg tgtcggtcac cagctgcgtg gtttccgcca
     9781 tgacatacgc tttatatgca gtaattgcac caccaagact taacagcgca tcactttgcg
     9841 ccgcatccgc cgttgcctca cctttgacga tcaacttccc tttcaggtta gtcagcagac
     9901 cacaggtcat atcgtattcg ccaggctgta aattcgccgt cattttctgg ctaaaaccag
     9961 gggcgatatt ttcccgctct tccaccacca tcacgccttt gaggatctcc cactccagcg
    10021 ccttctggct atggttctga ataatgaact gtgttttccc ggcgttaacc gtaatggtca
    10081 tcggttcgca ctgcttatcc gtcacggcca ctttgacctg cgtcacatca gcggcgttag
    10141 ccataaaagc agaagaaaac agcgcagcca cgctcaactg caatgcgtta cggcggaagt
    10201 taatggtcat gacgagacta tccctttaaa gtatgttgta actaagcaag agtttgcgtc
    10261 gtatttgtta cgcggagcga gacgctgtcg cccctgcgcg tggtggcaga gcaaatgcca
    10321 ccagcgccgg gatgagataa ataaaccaga cggcgacttc gctgacgctc ggtgcttcct
    10381 gatagccaaa aatcccttcc atcagcgtgc caaacagcga gtgagttgag agcactgcgc
    10441 tcatatcgaa ggcgatttcc tgaaagtggt tccacaatcc ggcttcatga aatgcgcgaa
    10501 tggcaccagc cgccagccct gcggcgacga agagaataaa caggctggtc catttaaaaa
    10561 atgcaccaag attgaggcga ataccgcccc agtagagcag aaagccgagc accacggctg
    10621 tagcaagacc gagcattgca cccagcggcg gccagatccc gacatcttgt tgaaatgccg
    10681 ccagcaggaa aaagaccgac tccagccctt cccttgcaac ggcaaaaaag accatcatca
    10741 ccagcgccca gccatgatga tttccacgct gcaatgcgct atcgactgcc tgttccagtt
    10801 gcactttgac gttgcgcgac actttgcgca tccagaaaac catccaggta aggatcacca
    10861 cggcgatcac cgccacgata ccttcaaaca gttcctgttc tttttgcgga aattcgccgg
    10921 tggtttcgtt aatgaagatc cccaggccca ggcacaacgc agcggcaagc aacacgccaa
    10981 tccacatcac accaatccat cggcctcgct gggtacgctt aagatagctg gcaatcaaac
    11041 tgacaatcag cgcggcttca agtccttcgc gcaacataat gagaaacgga acaaacatgc
    11101 cacccaccct taaacgtttt tctgagtcaa tctatgcaaa gaaaagtaaa tcgcagtgat
    11161 agcgattatc attacggcaa agaaaaacac aagcggaatg tttataaaat gatgctggaa
    11221 tgtaaacaaa gagaggtaat gccaggggat ataaacgatt attccagggt agaaagatgg
    11281 gattgtttgc aaagaaccat gtgttcttgc cggatgccgc gtaaacgcca tatccagccg
    11341 acaaaactgt ccagactcaa tgcatcagag cttccatgta aaactgataa gcgggtgtat
    11401 caagcaattt cgtccatccg cgattatcaa atctaacttt ttgcccggaa cataataaca
    11461 gctcgggtgt tggtggcgtc aaaatcgctg aagcgttccc cgaaggccgg gacagcccac
    11521 atggatgtgg gctgagggcg cgtcttacag gacgttacct cgcgcccgac ccggtagccg
    11581 aaggggataa gctgaaggta ccgcgcagcg gcgattttgt tcgccagagc ccgggggtgc
    11641 acagggggcg gcggcgattg gccgccccct gcgcgctccc tgcaccattc aatttatatg
    11701 gcgaggcaca ttaagggagc gcaacccgct gctgaagcca aacaaaaccg ccgcaggcta
    11761 agtcccttag ttttcctgca accgtgacgg cggagccgaa tgatagtgcg catcggcctc
    11821 ggcaaagcgt ttttgcatcg ccgctgacgg cgctttaccc agcaaactaa acactacaat
    11881 cccgatactg ccgaagatga agcccggaat aatttcgtac agtcccagcc agccgaactg
    11941 cttccagacg ataactgtca gcgcaccgat gatcatcccc gccagcgcac cgttgcgcgt
    12001 catgcgtgac cacatcaccg agaacagcac caccggacca aacgccgcgc caaagcctgc
    12061 ccacgcgtag ctcactaagc ccagcacgcg gttttccggg tttgccgcca gcgcaatcgc
    12121 caccagcgcc accaccagca ccatcacacg tcctacccac accagctctt tctggctggc
    12181 ctgtttacgc agaaacgctt tgtataaatc ttcggtaatc gcactggagc acaccagcag
    12241 ctggcaactt aaggttgaca ttaccgccgc cagaatcgcc gacagcagaa tcccggcaat
    12301 ccacgggtta aacagaattt gcgccagttc gataaacacg cgttcagcgt tctggtttac
    12361 cgcacctgct accgccggat gctcgttaaa gtaagcgata ccaaagaagc caacagccac
    12421 cgcccctgcc aggcaaagga tcatccaggt catactgata cgacgcgcat ggacaatgct
    12481 gtggtgagaa tctgccgcca taaaacgcgc caggatgtgc ggctgcccga agtaacccag
    12541 cccccaaccc atcaatgaga taatggcaac aaagttcagc cctttgagca tatccacgtt
    12601 ttcgatgctc ttttgtttga tcacttccag cgagtcacca aagccaccga cactgataat
    12661 gacgataacc ggcgtcagga tcagggcaaa aatcatcagg ctggcctgta cagtgtcagt
    12721 ccagctcacc gcgaggaaac cgccaataaa ggtgtaaagg atcgtcgccg cagctcccgc
    12781 ccacagagcc gtttcgtagc tcatgccaaa ggtactttca aacagacgcg cgcctgccac
    12841 aatgcccgaa gcgcaataaa tggtgaagaa cagcaaaata accagcgcgg agataatgcg
    12901 caaaatgcgg cttttatctt caaaacgccc ggtgaaataa tccggcaatg ttaaggcgtt
    12961 attattgtat tcggtatgca cacgcaaccg cccggccacc agcttccagt tgatccacgc
    13021 gcctaatgtc aggccaatgg cgatccagct ttcggaaatc ccggaaagaa aaaccgcgcc
    13081 aggcaacccc attaacagcc agccgctcat atccgacgca cccgccgata atgccgtcac
    13141 gaatggccca agactacgac cgcccagaat atagtcgtca aagtttttcg ttgatcgcca
    13201 ggcgataaac ccaatcaata tcatgccaaa gatatagaca caaaatgtca ccaacatcgg
    13261 tgtgctaata gccatctaaa gtctccaaaa aattattatc ggcaatgtcg aaacttgccg
    13321 ttatatctgc caccggaacg gggtaacaga gtttattttt taccagggcg accgtatcct
    13381 gccggaagcg ctagttattc acaatcgatt taacacacca tttacattaa attttagtgc
    13441 tcagcgacac tatttttcat caggttgcac tctctcacat tttttgcggt tgcacctttc
    13501 aaaaatgtta actgccgcag agaaaaagtc tgagttattt ttttaatccc tgtcatatcg
    13561 atttctttta ttaacatttc attcattttt aagcttgcta cgcaggtcac atttaacatg
    13621 gttgcacaaa gttgcaacat catggatatt tcacgataac gttaagttgc acctttcaga
    13681 acaacaggag taatggcatg ggaaccacca ccatgggggt taagctggac gacgcgacgc
    13741 gtgagcgtat taagtctgcc gcgacacgta tcgatcgcac accacactgg ttaattaagc
    13801 aggcgatttt ttcttatctc gaacaactgg aaaacagcga tactctgcca gagctacctg
    13861 cgctgctttc tggcgctgcc aatgagagcg atgaagcacc gactccggca gaggaaccac
    13921 accagccatt cctcgacttt gccgagcaaa ttttgcccca gtcggtttcc cgcgccgcga
    13981 tcaccgcggc ctatcgtcgc ccggaaaccg aagcggtttc tatgctgctg gaacaagccc
    14041 gcctgccgca gccagttgct gaacaggcgc ataaactggc atatcaactg gccgataaac
    14101 tgcgtaatca aaaaaatgcc agtggtcgcg caggtatggt ccaggggtta ttgcaggagt
    14161 tttcgctgtc atcgcaggaa ggcgtggcgc tgatgtgtct ggcggaagcg ttgttgcgta
    14221 ttcccgacaa agccacccgc gacgcgttaa ttcgcgacaa aatcagcaac ggtaactggc
    14281 agtcacacat tggtcgtagc ccgtcactgt ttgttaatgc cgctacctgg gggctgttgt
    14341 ttactggcaa actggtttcc acccataacg aagccagcct ctcccgctcg ctgaaccgca
    14401 ttatcggtaa aagcggtgaa ccgctgatcc gcaaaggtgt ggatatggcg atgcgcctga
    14461 tgggcgagca gttcgtcacg ggtgaaacca tcgcggaagc gttagccaat gcccgcaagc
    14521 tggaagagaa aggtttccgt tactcttacg atatgctggg cgaagctgca ctgactgccg
    14581 cagacgcaca ggcgtatatg gtttcctatc agcaggcgat tcacgccatt ggtaaagcgt
    14641 ctaacggtcg tggcatctat gaaggtccgg gcatttcaat caagctgtcg gcgctgcatc
    14701 cgcgctacag ccgcgcccag tatgaccggg taatggaaga gctttacccg cgtctgaaat
    14761 cactcaccct gctggcgcgt cagtatgata ttggtattaa catcgatgcc gaagaggccg
    14821 atcgtctgga gatctccctc gatctgctgg aaaatctctg tttcgagccg gaactggcag
    14881 gctggaacgg catcggtttt gttattcagg cttatcaaaa acgctgcccg ttggtgatcg
    14941 attacctggt tgatctcgcc acccgcagcc gtcgccgtct gatgattcgc ctggtgaaag
    15001 gcgcgtactg ggatagtgaa atcaagcgtg cgcagatgga cggccttgaa ggctatccgg
    15061 tttatacccg caaggtgtat accgacgttt cttatctcgc ctgtgcgaaa aagctgctgg
    15121 cggtgccgaa tctaatctac ccgcagttcg cgacgcacaa cgcccatacg ctggcagcga
    15181 tttatcaact ggcaggtcag aactactatc cgggtcagta cgagttccag tgcctgcatg
    15241 gtatgggcga gccactgtac gagcaggtca ccgggaaagt tgccgacggt aaacttaacc
    15301 gaccgtgtcg tatttatgcc ccggttggca gacatgaaac gctgctggcg tatctggtgc
    15361 gtcgcctgct ggaaaacggt gctaacacct cgtttgttaa ccgtattgcc gacacctctt
    15421 tgccactgga tgaactggtc gccgatccgg tcactgctgt agaaaaactg gcgcaacagg
    15481 aagggcaaac tggattaccg catccgaaaa ttcccctgcc gcgcgatctt tacggtcacg
    15541 ggcgcgacaa ctcggcaggg ctggatctcg ctaacgaaca ccgtctggcc tcgctctcct
    15601 ctgccctgct caatagtgca ctgcaaaaat ggcaggcctt gccaatgctg gaacaaccgg
    15661 tagcggcagg tgagatgtcg cccgttatta accctgcgga accgaaagat attgtgggct
    15721 ttgtgcgtga agccacgccg cgtgaagtag aacaggcgct ggaaagtgcg gttaataacg
    15781 cgccaatctg gtttgccacg cctccggctg aacgcgcggc gattttgcat cgcgctgccg
    15841 tgctaatgga aagccagatg cagcaactga ttggtattct ggtgcgtgaa gccggaaaaa
    15901 ccttcagtaa cgccattgcc gaagtgcgcg aagcggtcga ttttctccac tactacgccg
    15961 gacaggtgag ggatgatttc gctaacgaaa cccatcgtcc attagggcct gtggtgtgta
    16021 tcagtccgtg gaacttcccg ctggctattt tcaccgggca gatcgctgcc gcactggcgg
    16081 caggtaacag cgtgctggca aaaccggcag aacaaacgcc gctgattgcc gcgcaaggga
    16141 tcgccatttt gctggaagcg ggtgtacccc ctggcgtggt gcaattgctg ccaggtcagg
    16201 gggaaaccgt gggcgcgcaa ctgacggggg atgatcgcgt gcgcggggtg atgtttaccg
    16261 gttcaaccga agtcgctatg ttactgcagc gcaatatcgc cagccgcctg gacgctcagg
    16321 gtcgccctat tccgctcatc gctgaaaccg gcggcatgaa cgcgatgatt gtcgattctt
    16381 cagcactgac cgaacaagtc gtcgtggatg tgctggcctc ggcgttcgac agtgcgggtc
    16441 agcgttgttc ggcactgcgc gtgctgtgcc tgcaagatga gattgccgac cacacgttga
    16501 aaatgctgcg cggtgcaatg gccgaatgcc ggatgggtaa tccgggtcgc ctgaccaccg
    16561 atatcggtcc ggtgattgat agcaaagcga aagccaatat tgagcgccat attcagacca
    16621 tgcgtagcaa aggccgtccg gtgttccagg cggtgcggga aaacagcgaa gatgcccgtg
    16681 aatggcaaag cggcaccttt gtcgccccga cgctgatcga actggatgac tttgccgaat
    16741 tgcacaaaga ggtctttggt ccggtgctgc atgtggtgcg ttacaaccgt aaccagctac
    16801 cagagctgat cgagcagatt aacgcttccg gctatggtct gacgcttggt gtccatacgc
    16861 gtattgatga aaccatcgcc caggtcactg gctcggccca tgttggtaac ctgtatgtta
    16921 accgtaatat ggtgggcgca gtggttggtg tgcagccgtt cggcggcgaa gggttgtccg
    16981 gtaccgggcc gaaagcaggc ggtccgctct atctctaccg tctgctggcg aatcgcccgg
    17041 aaagtgcgct ggcagtgacg ctcgcgcgtc aggatgcaga gtatccggtc gatgcacagt
    17101 tgaaagccgc attgactcag ccgctaaatg cactgcggga atgggcagca aatcgtccag
    17161 aattgcaggc gttatgtacg caatatggcg agctggcgca ggcaggaaca caacgattgc
    17221 tgccggggct gacgggtgaa cgcaacacct ggacgctgct gccgcgtgag cgcgtgttgt
    17281 gtattgccga tgatgagcag gatgcgctga ctcagctcgc cgccgtgctg gcggtgggca
    17341 gccaggtact gtggccggat gacgcgctgc atcgtcagct agtaaaggca ttgccatcgg
    17401 cagtcagcga acgtattcaa ctggcgaaag cggaaaatat aaccgctcaa ccctttgatg
    17461 cggtgatctt ccacggtgat tcggatcagc ttcgcgcatt gtgtgaagcc gttgctgcgc
    17521 gggatggcgc aattgtttcg gtgcagggtt ttgcccgtgg cgaaagcaat atccttctgg
    17581 aacggctgta tatcgaacgt tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca
    17641 gtttaatgac gatcggttaa acaggtagcc ggaggatgtt acaacctcct ccggcatctt
    17701 taacgtggtc gaatcccctc aataataatc cgctgcacgt tttcaaccgt ttgattgaaa
    17761 aatatctcat cgcgcaacgt cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg
    17821 taatgttgag tggaagccca aatcatgaaa atcaaatgct gcggatcaat cggcgcgagt
    17881 ttgccgcttt tgacccaacc ggcaatcagc gccgatttct catcaattaa tgctttcaaa
    17941 tcacccgtca gttcatccat taacagcggt gcgcctgcca gcatctccat acaaaacaga
    18001 cgtgaagcct gcggataatc gcgggagact tccagcttca gacggatgta ctcttttatc
    18061 gccgccagcg gagcgaaatc ttcacgaaac gcttttaacg gtgccagcca gatatcgaga
    18121 atctgccgca gcacagcaat atacagcgcc tcttttgacg gaaagtaata cagcagattg
    18181 gtttttgaaa cacccgccaa ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat
    18241 tgtgaaaaag tgtccagtgc tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc
    18301 gaacgtttac ccgttgtttt cactgcgcct tgcgtcatgc gctctcccct cttgtagatt
    18361 ggcaacagga tagcaaaacc cccgcccggc ctcgaccttc tgcactctca tcgcgcacag
    18421 atgcatgttt tatgtgcaac tgttttgacc gtttagtcca ctttttatca gatattttag
    18481 ataacgtaat gttaacatat taataacatt aactttttaa aaactggcat ccgctttgca
    18541 aacaagccaa tacgcggctg aaaaggatat gcaggatgca gcgccccgtc tgactttcac
    18601 tttacgagac gaagagaggt tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa
    18661 cggctggctc atttcgaccc acgcgccgca gtacatgccg acctttgaac tgaataaagc
    18721 cattgtgcag aaagcggagc attaccattt cgatttcgcc ctgtcgatga tcaaactgcg
    18781 tggctttggc ggcaaaactg agttctggga tcacaacctt gagtcgttca ccttgatggc
    18841 gggtctggca gccgtgacct cgcgcattca gatttacgcc actgctgcca ccttaacgtt
    18901 acctccggca atcgtcgccc gtatggccgc aaccatcgac tccatctctg gcgggcgttt
    18961 tggcgtcaac ctcgtgactg gctggcaaaa gcccgagtat gagcagatgg gtatctggcc
    19021 tggcgatgac tatttctccc gtcgttacga ctatctcacc gaatatgttc aggtgctgcg
    19081 cgacctgtgg ggcacgggga aaagcgattt taaaggcgat tttttcacca tgaatgattg
    19141 tcgcgtcagt ccgcaaccga gtgtccccat gaaagtgatc tgcgccgggc aaagcgacgc
    19201 tggcatggcg ttctccgctc agtatgccga tttcaacttc tgtttcggca aaggcgtaaa
    19261 tacacccacg gctttcgccc cgaccgctgt gcggatgaaa caggccgcag agcaaaccgg
    19321 gcgcgacgtt ggctcttatg tgttgtttat ggtgattgcc gatgaaaccg acgatgccgc
    19381 tcgcgccaaa tgggaacact acaaagcggg cgcggatgaa gaggcgttaa gctggctaac
    19441 cgaacaaagt cagaaagata cccgctccgg tactgacacc aacgtccgtc agatggccga
    19501 tcccacttcg gcggtaaaca tcaatatggg gacattagtc ggttcttacg ccagtgtcgc
    19561 gcgcatgtta gatgaagtcg caagcgtgcc tggtgccgaa ggcgtgctgt tgaccttcga
    19621 tgattttctg tcgggaatcg aaaccttcgg cgagcgcatt caaccactga tgcagtgccg
    19681 cgcccatctc cctgcgctga ctcaggaggt ggcatgatga caaccttaac cgctcgaccg
    19741 gaagccatta ccttcgatcc gcagcaaagt gcgctgatcg tggtggatat gcaaaacgct
    19801 tatgccacgc caggcggcta cttagatctc gccgggtttg atgtctcaac cactcgcccg
    19861 gtcattgcca acattcaaac cgccgtgacc gcagcgcgag cggcagggat gctgatcatc
    19921 tggtttcaaa atggctggga tgaacagtat gtcgaagctg gcggacccgg ctcaccgaat
    19981 tttcataaat cgaacgccct gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg
    20041 ctggcgaaag gctcctggga ttatcaactg gtggatgaac tggtgccgca gcctggcgat
    20101 attgtgctgc cgaagccgcg ttacagcggt ttcttcaata caccgctgga cagcattttg
    20161 cgcagccgtg gaatacgcca tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa
    20221 tcgacgctac gcgatggctt ttttctggag tatttcggcg tggtgctgga agacgcaaca
    20281 caccaggcgg ggccggaatt tgcacagaaa gccgcgttgt tcaatatcga aacctttttt
    20341 ggctgggtca gcgacgtcga aacgttctgc gacgcgcttt ctcccacgtc ctttgctcat
    20401 atcgcttaag gagtttaacg atgccaaaat ccgtaattat tcccgctggc agcagcgcac
    20461 cgctggctcc cttcgttccc ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc
    20521 tggcttttga tcaacataat aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc
    20581 acgttctgga aaccatccgc aaggtgatcg agacggcggg tggcacgatg gcggatgtga
    20641 ccttcaacag catctttatt accgactgga aaaattacgc cgcgattaac gaaatctacg
    20701 ccgagttttt tccgggtgat aaaccggcgc gattctgcat tcagtgcgga ctggtaaaac
    20761 ctgacgcgct ggtggaaatc gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa
    20821 ctttcactct cacctccccc ttatgctgat gcgcccgtag tggtgttgat ttcgggtctt
    20881 gggggtagcg gcagttactg gttaccgcaa ctggcggtgc tggagcagga gtatcaggta
    20941 gtctgttacg accagcgcgg caccggcaat aatcccgaca cgctggcaga agattacagt
    21001 atcgcccaga tggcagcgga actgcatcag gcgctggtag ccgcagggat tgagcattac
    21061 gcagtggtcg gccatgcgct cggtgcgctg gtgggaatgc agctggcgct ggattatccc
    21121 gcgtcggtaa ctgtgctgat cagcgttatc ggctggctac gaataaacgc ccatacgcgc
    21181 cgctgttttc aggttcgcga acgattactg tatagcggcg gcgcgcaggc atgggtggaa
    21241 gcacagccgt tgttcctcta tcccgccgac tggatggcgg cccgcgcacc tcgccttgag
    21301 gcagaagacg cgctggcact ggcgcatttt cagggcaaaa ataatttact gcgtcgactt
    21361 aacgccctca aacgcgctga ctttagtcac catgcggaac gcatccgctg cccggtgcaa
    21421 atcatctgcg ccagtgatga tctgctggtg ccatcagcat gttccagtga acttcatgcc
    21481 gccctgcccg atagccagaa aatggtgatg cgctatggcg gacacgcctg caacgtgacc
    21541 gatcccgaaa cgtttaatgc tctgttactc aacgggcttg ccagcctgtt acatcaccgt
    21601 gaagccgccc tgtaaggaat tgctatgaac gaagccgtta gcccaggtgc gcttagcacc
    21661 ctgttcaccg atgcccgcac tcacaacggc tggcgggaga cacccgtcag cgatgagacg
    21721 ttacgggagc tttatgccct gatgaaatgg gggccgacat cagctaactg ttctccggca
    21781 cgtatcgtgt ttatccgcac tgcagaggga aaagaacgtc tgcgcccggc gctttccagc
    21841 ggcaatctac aaaaaaccct gaccgcgccc gtcaccgcta tcgttgcctg ggacagtgaa
    21901 ttttatgaac ggttaccact actgtttccc cacggtgatg cccgcagttg gtttacctcc
    21961 agcccacaac ttgccgaaga aacagcgttt cgcaacagct ccatgcaggc ggcctatctg
    22021 atcgtcgctt gccgtgcgct gggactggat accggcccga tgtcgggttt tgaccgtcaa
    22081 cacgttgacg acgccttttt tgcgggcagc acgctgaaga gcaatctgct gattaatatc
    22141 ggctatggcg atagcagcaa actttatgcg cgcctgccac gtctgtcctt tgaagaagcc
    22201 tgcgggctgt tgtaaggagc aaactatgaa catagttgat caacaaacct ttcgcgatgc
    22261 gatgtcctgt atgggcgcgg cggtcaatat catcaccacg gacggtccag ccgggcgcgc
    22321 cgggttcact gccagcgccg tctgcagcgt gaccgacacg ccgcccacat tactggtgtg
    22381 cctgaatcgt ggggcgtccg tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa
    22441 tacactgagc gccgggcagg agccgctttc aaatcttttt ggcggcaaaa cgcccatgga
    22501 acaccgcttt gccgccgccc gctggcagac gggtgtgacc ggatgcccgc aactggaaga
    22561 ggctctggtt tcgtttgact gccgtatcag ccaggtggtg agcgtcggca cccacgacat
    22621 tctgttttgc gccatcgaag cgattcatcg tcacaccaca ccctacgggc tggtgtggtt
    22681 cgatcgcagt tatcacgcgc tgatgcgccc tgcttgttaa cctccttaag gagacagctg
    22741 atggcaatgt tcggttttcc tcactggcag ttaaaatcga cctctacaga gagcggcgtg
    22801 gtcgcgccgg atgaacgatt gccgtttgct cagacggcag taatgggcgt tcaacacgcg
    22861 gtggcgatgt ttggcgcgac ggtgttaatg ccgattctga tggggctgga tcccaatctt
    22921 tccattttaa tgtcggggat cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc
    22981 cccagttatc tcggctccag cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt
    23041 aacggtcagg gcattaaccc gaatatcagc atcgcgctgg gcgggattat cgcctgtggg
    23101 ctggtttata ccgtcattgg cctggtggtg atgaaaattg gtacacgctg gattgaacga
    23161 ctaatgccgc cggtggtgac gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct
    23221 atcgcagtga aaagtgtatc ggcttcggcc tttgatagct ggatggcggt aatgacggtg
    23281 ctgtgtattg ggctggtggc ggtgtttacc cgtggcatga tccagcgatt attgattctc
    23341 gtaggattaa ttgtcgcctg tctgctgtat ggcgtgatga ccaacgtttt agggctggga
    23401 aaagccgtcg attttactct cgtcagccat gctgcctggt tcggtctgcc acatttttcc
    23461 actcctgctt ttaatggtca ggcaatgatg ctgattgcgc ccgtggcggt gattctggtg
    23521 gcggaaaact taggtcatct caaggcagtc gccgggatga ccggacgcaa tatggacccg
    23581 tatatggggc gggcattcgt aggcgatggt ctggcaacga tgctttccgg ctctgtcggc
    23641 ggtagcggcg tgaccaccta tgctgaaaat atcggtgtga tggccgtgac caaagtctac
    23701 tcgacgctgg tgtttgttgc tgctgccgtc atcgccatgc tgttgggctt ttcgccgaaa
    23761 tttggcgcgc tgatccatac cattcctgca ccggtgattg gtggtgcttc aattgttgtg
    23821 ttcgggctga ttgccgtcgc aggcgcaagg atctgggtac aaaaccgtgt cgatttaagc
    23881 cagaacggta atttgattat ggtcgcagta accttagtgc tgggcgcggg tgattttgct
    23941 ctcacgctgg gcggttttac gttgggaggg atcggtacag caacctttgg cgcgatttta
    24001 ctcaatgcgt tgttaagcag aaggttggtt gacgttccac cgccagaagt tgttcatcag
    24061 gagccataaa cagaaaaccg ccggggtaac cggcggtgat gactttttga cgagcttcag
    24121 acaaaaatgc attcgtcact gattagtccg gctagttgtc acttttaccg tgactgctct
    24181 tgccaccttt tttacctgct tcagatgcgc gctgcgggtc attcttgaaa ttccccccgc
    24241 tgtgctgtcc acctttttta cctgcttctg atgctctttc gcggtcttct gcaaaattgc
    24301 cggaaccgcc tcgatggttt gccattactg acctccgtct gggtatagac atcatattgc
    24361 atagaattaa cgcggcgtat ggcggctgcc atattttcgc tacgtgggac taagcttagt
    24421 gaactgacgc gggcagaccc gcgtttggta atatcctgca acagcttctt agcgagaata
    24481 gatatcatct ccaataatta tccataagcc gctcttatga ttaaaaaaac gcgatgagat
    24541 aaaaatgtgt cattttgcga caaaattacg tgcttgtgaa agttgttata aatcaaataa
    24601 gtggttgtga aatttgcact ctgaaaagga cgtcttatct ttaaataagt ggtagcgaat
    24661 cgctacggaa tagagataac acgaggagtg gttagaaatg gctaaagttc tggtgcttta
    24721 ttattccatg tacggacata ttgaaaccat gaagcattaa aaacacagaa ttaccgaaga
    24781 tcgtccaaga gcatatactt ttcaatatgt tagacctcat tttgaccata aagaaatcga
    24841 ttttacaccg ttctttatac gttctttttt gccccaaata tgccccaaac aaccacactt
    24901 tgccccaata gcgccccaaa gatttaaggg gatttaagta tatatttcag ataattaacg
    24961 caagtttacg atgtgaattt acgttaattt tctgggtagg gtatttaccg tcattcgttc
    25021 atcaatattt ctgcatattg acaaatacgc ctgagctatc gcgggagaaa tgcaatgaaa
    25081 gcagcaaaag gcgatgttaa tcaccctgct catctctgta ttaccgtcat tgtgacggca
    25141 ctgataacga agaaagacct ctgcaaggga ggaatccctc gccacctctg atgtgtcagg
    25201 tatcctcacc ctccccaatc acattagccc cgcacttccg tacggggcca tttctgttaa
    25261 tccactccca cttttttgtt gtactcgcgg ttaaaccgat ccacggcaat tttcatatcc
    25321 cgctctaccg atttcaccat cgccgactgt tgcgccagac tgagagtact gtcggcgtaa
    25381 atggcatcac gctgtttacg caaatccttc agtctcttcc gggtatcctg cataagcccg
    25441 ttcatcgaca attttccgtt gttctcgtca atgaacgccg ttctttctgc gccggtcaga
    25501 ctcttcagct ctgcgtgata ctgcgcaacc tctgtcatcc ggtcgtacat cttctgctgg
    25561 tcaacatacg gcatcacctc acctgaaatt ttccccagga aaggcacctg ctgttccggt
    25621 atatcaatac cgttcagcga tttcaccgcc gcatccgtgg ttttggaaat gaagcgccct
    25681 gtaccaccag agatatagtc cacccagtat ttcagcgatt ccggtgtgat atccaccgcg
    25741 cctgaacggt actggctgcc acctgagaac gcattcagcc aggatgcaaa cgccttgtac
    25801 gcttctggcg ttgaacgtct tcccagctgg ctgtcaggtt ttggtgtacc aaacggcatg
    25861 ttctcctggt aaatctgcgc ccccatgaag ttttcattca tggcaaggtt cgcaaacgga
    25921 cgcagaatgg tcggcgctgc atttttcagc aatgccccgg acagtgtttc cgacgtctca
    25981 ctgccaatcg ggctgaatgc accaagcaca ccaccaacaa cattaccggc agcacgggac
    26041 gccgtcaggt cacccgccgc cacaccttca gcggtatgcc cgagcaggaa gaaaacgttg
    26101 tacccgtaag gcagaggaat actccagtac tctccggcct tgcccccgaa caccgatttc
    26161 ataatgacga ggttacgctc tttcacatga gacggcacct tgtcatacca gttaaccccg
    26221 tcatcatcct cccccgcaac actgcggtta agcgagccaa gcagataacc cgctcccaca
    26281 gctgcaagcg cgattttctg cggtacattg agattcttcc agcgaaggcg ctccagtaac
    26341 ggcccgtcgc cattaagatg tccgagcgtt ctcaccaggt ttgcggtccc ctgaatgctg
    26401 gcgttggcga acatgtacag cgagttcatc agcgctccct gctcaccacg acggttaaag
    26461 ttcaccgtca tgtttttggc aagagacgcc gcctgctggc gtgacaaacc ggcatcacgg
    26521 gcgtgcttat aggcagaaag acgcagagcg ttttcaaccg caccgttggc atcctcgaca
    26581 aggttaagga acgaattcca tgcaccgata ctctggcctt tccatccccc cttcgccagt
    26641 gagacaaggc gatccatttc cttctgctgg ccttcaaggt cacccatgtt aaaccagccg
    26701 gttttccctc cgtcctcaac aaactctttc cacaccttct gccactgtgc accttttccc
    26761 gtgagggttt taccacgcag actggcgtat acggctgaca tggcagaacg gctgtctttc
    26821 accacagcca gggcggataa gttatccagc cctttcagtt taccgtcgct ccttccctgc
    26881 tccgccttca ggttcatcac cgccgtctgc acgtcacgga tgaagttact gaccaggaat
    26941 tccgggttat acgacgtgtt caccgttgcc aggaagcggt taactttccc cagcgtacga
    27001 acaaaggcat tgctggtttc cggtcccata ctcttcatcg cacgcatcag gcgcggatca
    27061 tggagtttga tgtagtacgt tttgccgttc tttttggtgg tgaagtaccg gtctgccatc
    27121 attgccatcg gtacagggcg ttcgacaact tcgcgaatgg tttcaccagt ttcctggtcc
    27181 ttgcgctctg caatcgtccg catggtatcc ggtctgtcat cggtgaatac ctgccagtaa
    27241 tccttgtcgg gattatcctg caccagtttc aggaaggcgt tacccacttc gtttttgcga
    27301 tggcggatca gcgattcgct caggtcctgt atcgcctgag tggacggaga ctgtgcccgg
    27361 gatgcacgcc ccatggcctg cctgctttca cgtccgccga tggtgaagcc cttacctgta
    27421 cggggcagtg acaccacacc gtcaacatcc tgacctttca ggggaacgta gtaacggtag
    27481 gcgttctgcc aggcatccac cacaccgttc tcttcaagtc cggcctcacg gataagctca
    27541 cggcgacggg ccagcatatc gtcaataatc cctgccagac ggtcatactg tgcctgtttg
    27601 ccactgttac gtacacgctg catgatttcc gccgcttccg cgttggtcat ccccgaaccg
    27661 ccgtccggca ttttcgggtt gattttcgcg atatgcgcgt tacgttccgg cgcgtgacgg
    27721 gcgtagaggt actcatccag atcggcctgc gcaattttgt agtccgccag taatttagcc
    27781 agtggctgaa cgtagcgctc cttcatcacg ttcaggtcgt tttccgcctt cccgtggaag
    27841 agttcttccg ccatataagc gttgttactg tcgtccactt tgccgccagt tttacggata
    27901 ttctcctgaa cagctttcag cacctggaat ttatcctgca tctggcgcac aaaacgcgat
    27961 gcaattgtct cttccggtgt cagactgctg gtacgggagt aatacggccc cttgcgaata
    28021 tcttcaggat agagtatttt atccgcagag ccgcgataag aatgttcccc tttgggcaat
    28081 tggagccctc tgtgcagaag gttatcgcgg cttaattttt ctctccggta caacgttaac
    28141 ccggtcattt ccatactctt cagttttttt ccattttctg taccgtaaac agaagctacc
    28201 ttgttgattt ccagacgcga ccggacagcc ttcatgtgta ccgctgacac caccggatca
    28261 ccatttttat ccacggcatc aagcagcatc acaaccgcat ttctttctgt cgctgagcgg
    28321 taaattgcat ccggatcgtg catcagttcc ggtagtctct cgataacatc catcggcacc
    28381 acatgtttca caccattggt ggccttccgc acagtatcgc gggaaataac cagcggcaaa
    28441 tccggtgcac caaggtgacg caataccggc ggcgtacgcc cgatgtttac cgttaaatct
    28501 gtggtgcgca gagatttcat cattctggca aggtcatcac gataacgctc gccctcacct
    28561 tccggcactt tgaacggatc acgtttacca ctacgggagt actgagacga tgcgcccgcg
    28621 ccatcctcac gcggcgtgta accttcccgc acacgctggc ctaacgtacg gatggtctcg
    28681 cgaacaagtc tgatatcgtt cagttccgtc ggcttcagta accccgtacg acgcagtacc
    28741 cctttgacca gggcaacaac acgctcccat gccgccacga atttattcgg ctgtttctcc
    28801 gccatatgtg ccagaaattc acccgcctgt acttccggtg attccttacc ataggaagca
    28861 tcaaccttac gccaggcttc acggatggtg acgttatcac tgtcgcgggt tttcagcacg
    28921 gttttgataa tcgtctgata ttccgctggt gtgacaacat gctccatggc atggtgaatg
    28981 atctcgtgac gcagcttctc gcgaacggtc cgcccgtcag ggatgttatc cgccaccagg
    29041 acaatttctc gtttatccgg acgatagaat gcgtgcaccc tgccgtaacc atcgaacaat
    29101 tcacccgcca gcgcttcagc ctctttctgt gacttcacca cacggacctt caggtcactg
    29161 tccttaatgc cgctcatcac gccacgggca accgcttcaa cctgcgggac cgggctgcct
    29221 ttggcttccg cactacggtt aacatccgaa atgagattac cttcaggtgt gcgggtaacg
    29281 cccttacggg aataaaacgc aacgcccttg tccgtctcac gggttttcag ggtgcggaac
    29341 aggtgatcga atgcctcacg aataccgcca tccagttccg cattcgtcgg ataagcccag
    29401 gtgttatctg tgttgtgctc aggtgcctta cggatattga ccagataatc attctccacg
    29461 ccagccatac gcgccttatc ctgaacataa cgctcaaagg cacgtgccgc catttcaaca
    29521 tccgttgacc agtacggttt tgagcgcacc tcatcgagaa gcactgaacg acgcggcatg
    29581 tcactgtttt taatggcctg aatcacacct ttaaaagcat cgtaaacctc ctgacgtacc
    29641 ggatattcag catcaacata cctgccgtct ttaaatatgc gcctgacacg ctgtgcttcc
    29701 gtcataaagt cgccacctga cgtaattttc ccgtcattgg aaacgtcata acgaccaaaa
    29761 taattatcca gagaatggaa ccattcgtgc gccagcgcac ccggtccgtt accttttgtc
    29821 aggttgattg ccacctcacc cggctcatag tgtgctgccg ccttaccctt accacgggca
    29881 ccaaatgcca ggccaagacg accgttcagg gaaagcgctt ttgtcggtac attcaatact
    29941 tccgcaaggt catgcagcga gtcataagcc cggttcaaat cagcctgacg acgcggacct
    30001 tccacgtagt taccaaactg cacaccacgg aaaccaaacg catcactgaa ctgctccggt
    30061 gaaacatccc ctttacggcg ttctggtccg gtacggtcgc ggttggtggc gttacgctgc
    30121 tcctcacgcg aaatctcccg catctccttc acatgacgaa caagctcatc acgatgtgaa
    30181 tcaatgtact tacgcgcatc actggctgac ttaaagccac ctctcacccg cattttgttt
    30241 ttgccataag cgataaaaat atcgccactg cgggtattcc ggtaaacgtc aaagcggatt
    30301 ttgtcatccg gtgacggtgc cgttttttca tctcctttcg cctgtgactt ttcctccagc
    30361 tctgcaaacc agactttcgc ctttgtcagt aattcatccc tgctttccga gaaaaagagg
    30421 ttagtcccct tattgtcctt attgcgcagt gaataaagtt tctgtggcgg atcgtaacgc
    30481 ttccctcctg ccgcctgata cacacccgat accacccgat aagcagaagc cttgtccatc
    30541 tgtgagggtg gcagagtgcg taacagttgc caggtatccg cgtaacggga gggcattctg
    30601 ccttccatcc attctgcgag gcgtttcgcg ctgaccgttc cgttaagcat ttccgataca
    30661 ctgtgtcgta ctttttttac gctctccccc cagccagccg tattgtgttt cgtcttcgcc
    30721 ggaatatcgc tacgatacag cgctatcatt gccagggtgt cagcatcagc cccttcgctc
    30781 gccagttttg cgtagtccgg tttcgggaac agtttgctca gcggctgcgt ggcataatcc
    30841 ctgtcttcca gcgttttacc cagtgtttca gcaagctgtg cataacggtg tttggctgcc
    30901 cctttaattt cctcgccaaa gtcttcaatt tttttgcccc tgacttcacc tccgcgcaca
    30961 gtagcttccc tgtcagtaga gacagttgtt tcagattcag gtgcgattgt ctcaggctgc
    31021 gggatgtcac gatttcctcc ggcctgtcga cgtacttctg cctgctgtgc gagctcaggc
    31081 aatgagttac gaacagttcg gggaagctga tcaggctgag gcatacggac attgtgtgta
    31141 atgtccggag ccggtagccc ttcacgtacc ggtgctgtcg cgttctgcgc atccggtgac
    31201 ggcagaccac gacgaaccat ttcaccctcg agtgtttccc cctggcgacc agcggcgttt
    31261 tccggtgccg gagcttgtcc tttctggaaa ctctggccct taacctcacc ggttgtggta
    31321 aaacgaccac cacgtccggc ctgattttca tccggcgtac gcgccacttc tcccggtaac
    31381 ggatatccct gtccgggatg aatatcgccg ggagcgggca ggcgcggacg ctcagttaac
    31441 tcctgtgctg tcggaccggc atcaccttca gccatctgtg aacgtacaag ctcctccgcc
    31501 gtcggcgcgt tgccacgggc aaggcgggcc tgtacctccg tatcatcccc cgtgaaacca
    31561 cggaatcgcg ggtcacgcat gaacgcgggc tgctccatcg ggtcagcatc agcaatacgc
    31621 tctctgtcag ccagggtgtt aatggtttcc gttgccacat cttcagagaa cgcaggatta
    31681 tccatttcaa cggcatactg attctcgcct ttcttcacta cggacggttt caggccggtg
    31741 gcggcggcat tacggaaaac atcactcccc atggcgcttt tctcatccgt gaaataacgg
    31801 ttgtccgggc gcaccttctc aatgcgttcc gctggtctgg acgcaccatt cccgttaatt
    31861 tcaatgcgga cttcattcgg gtcccggaac cgcaccgtcg ggtaaatgct cccgttacca
    31921 tcctccgcat tttcaggctg tgactgtgct tccggcgtca cttcctgttg tgggcgaatg
    31981 acttctcgta ttgcctgaaa ctgttgttct tcagtctcgg tgcgcccctc tttctggctg
    32041 agtctgcggt attcctcaag cagttcagaa cgcggcttcg ccttcagctc attcatcaca
    32101 gcctgtcgct tcgtctgttc gtccagttca ttcaacaact ggctggcagc ttcccggcga
    32161 tgagctgcgg atgcgtcacc ctctgttgcc atatccgcat cagcatactg ctccagaagc
    32221 tgctcgcgat tcatgccctg catggattca cgctgctgcg ccaccggatc aacagattct
    32281 ggttgcgggg ctgcgtcgtc ctgctgcacg gtttcagcat cacgcatggc ggtttcttct
    32341 gcggcctgac gtctgccacg atatccggca accgcaccga atggagcccc catcgcagca
    32401 ccaagggcag caccttcgat cgttgcgtca gccacgccct cccatggcga tacatccatt
    32461 ccggcggtct cacgcaatgc cgtgttttcc tgatagcgtg aatagccgcc ctgcgccgca
    32521 ttaatcgccc cctgtgcagt ggcatttctg acaatgccgc ttttaacggt cttcgctgtg
    32581 cctcgtgtca ccagattaaa cagttgtgcg tcacccagtt ttgccgccat ggcattcaca
    32641 gccagcaatt caggatcggt tgccagctgc gcgcgcacct catcggcaac acgctctttt
    32701 gccagatcca ttttctggcg atcagtaagc tgtgcgtgct gcgggtcggc gtcaatggac
    32761 aaaaacgtct gctgaaattt cggtgactgc gccagctcag agtaatcagc attaagaaca
    32821 gcatctgctg ccgccattgc actctgcccc tgtgcactta cagtggaatg ggtgatcagg
    32881 cccgcctgga ataaatccgg cattttttta tcgacagctt ctgctgccag tgccgtggct
    32941 ctttccggct gcatccctgc cgcgatgtat tttttctcca gcccggcggt cagcattttt
    33001 cgcagtgtga catcacccac ctttctagcc acaccgccag caaccatatc aggtacaagc
    33061 gcaccaatca ggtttacacc cttcgccacc cagaccgcag aatcattata gccttcggtc
    33121 atcggcgtat tcagcgcacg aactgcaccc ggcgacatct taccggtcag ccattcatca
    33181 gtggctttgg caccatcact gacagcctta ccggttgcct tcagaccttt accgacagta
    33241 tcagtaaccg cgtttttgcc atcaggcagg gtatcgatga cctcatcagc ccccctgctg
    33301 ccaccggcaa aaatatcctg cacagttgcg acacccggca gccccatacg gctaaactca
    33361 tttaaaatac gcgcccctgt ttttaccggg ctctgaatca tcgcatcacc gagtccacgg
    33421 gccatttccc ctgttccccg gacggactgg gcgaaacctt tacccattgt tggcaataca
    33481 tcgcccaggc tgaacgacgt actgttatcc ttccagcgat tcggatcaga gaaaaatgct
    33541 tcatagctgt cagtttcgcc gggttgctga atgttcaggc tgttacgatt ctggttaccg
    33601 agttgcgcct caggacgctg ttcctctgaa taggccatac agactccata aaaaaacccg
    33661 gcacaatggc cgggcatcag gaaatggtat taatggaata tcgtgaatta ctgggcgtaa
    33721 ttctgtttca gtccacggat aaactgagat gcagatgaag cactttcatc tctggcctgt
    33781 tcccccctgg ccttttgaat acggagaaaa ttcgcatacc cgttctccag caatttctga
    33841 ttctgaggtt ccagcatttc aggctgacgg gccgctacat ttctggcaaa tgacagctta
    33901 tcaggatcat ctcccgccca gttgataacc tgttgctgaa gtttttgttg ctgaattttc
    33961 tgttgatgcg gttgtgaacc agtcgcagca taatattcat ccaccgcagc ctgagcatta
    34021 ccgccattct tgatcgcgtc cgccgctacg ttgctggccc ccttctgaag ctctttcaga
    34081 gaaagtccct gctgcttcgg cataaaataa ccataatttt tcgatatctc tgcaaatttg
    34141 ctgcgatccc tgacctgagc gatagcctta tcaacgggta tcgccagcac ggtttgatca
    34201 tcagggtgcg cactgccgta ttctgttaca ggtttatgcg cggtggagcc atcgctgtat
    34261 gtgagatcaa ggccaataag tacgtaccct tcctgctgcg ctggtacgat actgccaatc
    34321 ctggcctctt ttatcgtttt ttccccggtt gaatcaggca tgccaatacg ctgttgtagt
    34381 tccggggcaa acacgccgga aagcacatcg agattttccg gggtattcag cgaatcgatc
    34441 gccatgtccg gcttatcacc aaagattttt tgcaggttga tcacggcctg acccgctttc
    34501 gctgcatagc cttcgtttga cataagaatt aatggatgtc cctttggcag ctgcccgaac
    34561 agacgcgttg cagcctcctg atcgccagcc tcaattgctt ttccaagcgc ttccattagt
    34621 ggctggctat gggcaatcat atcgttatac tcaagccgtc gctggttgta tttctgcaac
    34681 tggagtcgct gctgctccat acccagtgac gcattccggt aattctggtt agcgttaaac
    34741 tccctttcct tcagtgcata attacggtcg tcaactttgg ctctgtaatc aaagttccgc
    34801 tgatcaacgt ttttattatg ttcaaactga gactgcgcaa actcaaaatc gcgctcgtta
    34861 tttttctgtt gccgggcaag ctgtgcttca cgcagaccaa gctccttacg acgggtcatt
    34921 gcctggtcaa cggtgctgaa tccggcaagt aacccctgtg caaatccgct cattcaccac
    34981 tccttaaaac aaagaaccag caatgccgcc aattactgct ccagcaacag caccaacagg
    35041 accaccaacg gatgcgccaa tagccgcccc cgtaccaatg cccgtaccga tattctgctt
    35101 gttctgcgct ttctgttgcg ccgccatctg tttgttcgca gcctcaattt cttcacgtcg
    35161 tctgtctgcg tcacttattc cctgtaatgc ctcacgccgc gactgatttg caatatccag
    35221 taaaccgtat cccatattgc ccccttacgc tgccaccagt tggccgccaa cactcagttt
    35281 ctgtcttgcc ggtgcagaag cccccgtcag aatattcatc tggcgatcct gttcggcttc
    35341 acggatacca tttttcgcgc cagcaattgc cagggcatta cgtaacccca gcgtattact
    35401 gtcgggatta tccgggcggt ttaccccgta tctcgccatc tggttatcct gcgccatctg
    35461 cgctgtacgg agactggaag tggcaaggcc tcccacccgt gcaagctgtg cattcatcag
    35521 actgttgttc tccccaaggt cagccagcct tgctacacgg ggtaaatatc tggtgcgcca
    35581 gtcgtcgtat tgctggcggg tcagcgctgc ggatgtctgc cagtccccct gagggcgggc
    35641 tgctccggta taaccgtatc ttgcaagtgt ttcgtattta ccgtactcca taatcacagt
    35701 ctccagttct gagcctgatg ctgaatggca ttagcgccgg tgccaggtgt tttagcaccg
    35761 ccacttcctc cgctaccgcc agccttatgc atcgcatatg cacctgccgc acccagacca
    35821 gcgccaacaa gagaggctcg cccctgctgt ttcgtaaacg ccgcctgtgc atccgattta
    35881 gcttttgcca gactgctgtc tgccagagag ttaaaactct gtaacgcatc cgccttctga
    35941 ccagaaccga gagcggcaac atcctgtagc ccggcaacat acttatctgc ctgcgatacc
    36001 tgcccccgtg tggttgtgtc aatctgcccg gttacctgat cactctggtt cgcattcatt
    36061 accgcgttaa agcgaccact ggacgggtca acaccggact gagcaagatt acccgccagc
    36121 tcccttcgcg cttcaccaaa ctgtttctga taaccaagat ttgttgtgcc agcgatattg
    36181 tcgtactgct gctcactgtt aaggtcatcg accttttcca tgaagttatc ttcagccggg
    36241 cggaggatat ttttgtaatc ctgccaccct ttccaggcca cttcttcctg tgctatttgc
    36301 gctgctgttg gttttacttt ggtatcacca ccgcctttac ttccacccat aatggccccc
    36361 tggataacaa aaaaccctgc cggagcaggg tcagaatgtg aattacaatg ttggtttaaa
    36421 cacaatgatg aaaactgtag cagtcagacc gctatcctga acaccatgaa gccatcctca
    36481 tcatccggca ttcgctcaaa gcccagtcgt tttcccagcc ggataaatcc ccgccttgcc
    36541 gtatggaact cagcccagcg tccgccagcc agatgggtta atgtcttcac ctccggcagg
    36601 taacgctcaa cgctgttact ccccgtacac acgcccagca acaccagaac ataagggata
    36661 ccatcagcac tgagcacaga acgcagcacc aaaaagccat caggtgcctc aaaacaaaac
    36721 gcctgctttt taaggcaggc gtctttaact tcattcataa attcagggtt gcgggaattt
    36781 ctcacaacac gctgcatata ccagagaatt ttatcgttca ttctctcacc tgaaacgggt
    36841 gccatatcgg ctgaacctca gcaaccagtt gacggggact ctcgtccccg tcgcggtttt
    36901 cctactgctt acactgtaag aacgccgcaa actccgctcc ccacaaattc agccggaact
    36961 cacacagtga accgtgcaac atccagatgg tgaagataac cgtcatgcaa attgtgacgg
    37021 tgataagcga tttttgcgac atagcgcttg actcctttta cagagaggcg ctaaccttct
    37081 acttgcttaa ggtacgaagt gttagggcct cgggttaacg ttaagttgac tcggggcctt
    37141 tccacatcag gccttcaggt tcaccctcca gccatcagcc gaaaggcatc cacgcataat
    37201 ttacggtttt tgccctgtac gggcaataaa aaacccgcca tcacagcggg tagtaaacgg
    37261 ttaccggata caggtcagaa tttcaggcca atgccagcag taacgccact cgtgcgccag
    37321 tcgccagagc cggaaccttc gtaagccaca tccagtgtaa cactctcatt caggttaaac
    37381 tgtgcacctg cagcccacgc aagggaggtt tttttcgtgc tgttgctttc agaaaatccg
    37441 ccactactgt taatattgtc cttaattttc aggtcagcgc taactttagc aacgcccatc
    37501 cctgccatcg catataaact catgtactga ttaacccgcc atgaaggccc cgctaacaga
    37561 ctccagtaat tcgccctgat atccgttctg gcagaagctg ccggattctt aatcttcctg
    37621 gtatgatcgg ctgactgcac atcaataaat gtctgtgagt tagtgagaga acgcgtccat
    37681 gtaaaagagg tgataacgcc aaaatcatcc gttatctcgt agcgatacct gatattaatg
    37741 ccctgtggat ttttatcctt gccttcatat ccactgaccc gaccatctgt atattcgccc
    37801 aatgaagaaa agtagtttct gttgacgaaa tgactgaaag tctccctgtt atgagcagtc
    37861 gcatccttta caaaatcctt cagtcccgga aactgaaagt gcgcataccc cagagaaatt
    37921 gtatgctctc cttctgctgc atgtgcagat aaatttacac aggcaatccc ggagattgca
    37981 cacacaacca gtgttgctat actcttcatt taagatatcc tgtattcaga taacacgcta
    38041 aactttttaa taatacatct ttttagacat tttaaatgaa tattaaagac tcgcttcaac
    38101 taatcatata ctgatgtatt aataaccagg accttatcta tatacggctt tcgaatatcc
    38161 catgttgcac caccggaata atgctcacag gaatatattt tatttcctgt agcaccagtg
    38221 gatgtggtat aaatcggtcg atcataagga gttcttttcc agttataata cccgaccagt
    38281 gcaggcatga ttgcgcatgg atatccaagg tccttttcaa atttgatatc aacaggtatt
    38341 aacttcgcgt caagcagcat catttcgcca tgataaatca tctcaccgtc cgggttatac
    38401 atggcgatac catactcaga aggtggagta accatattcg cgaaagcata aaccgtcgta
    38461 acaccagggt tcgttcccct gacaattaca tgaagcctta gtgcatgata tccatcaatc
    38521 tgttcatgcg tgtacatgac atcggccttc ttctctgttc tgataaagaa aaaacaactt
    38581 ttgcctgacg ggattgatgt tttaaaaaaa gacttttcag tcgccggtat ggtgcctttg
    38641 ttgatcagac actgcggcgt aaaacctgga cttatccata cgctgccatc cggcttcata
    38701 atgctcaaac cgtacataac acttatcccc agaatgtata aatataagat cccataccct
    38761 gctcaagatt cgaccacgtc accgtattgt cattaatggt tatcttcggt actttccgat
    38821 ccgcaaatac attattccag ggaaataaac aacatacagc ctgcaatgat ttcccgtcgg
    38881 gtttattagt gtacgtcttt gagccagact ccgctgtaaa tctgtccagg aaaaatactg
    38941 gagtcagcac gcccgtaaca ttaacgttat ttctgttata aatggcaaaa ccgtattcca
    39001 atactcacct cctgatcagc gtaatctgcc tatgcgaaca gccagtcgtc cattctgatc
    39061 ataaacctca attttatcat tgcggatcac cagtcctaca ttctgattag agtaacgaat
    39121 tgtcagttgc ccttgtgacg taacactgaa aaggcctcca atattcaggt taccctgaga
    39181 atcaacctga aagtttccgt tctgaataac ggcactccgg ataactggcg aagtgatact
    39241 taccccggct tttacctcat ccgccacaac cttccgcgac accagtgttt caatcaccgc
    39301 gtcataaatc atcgctttcg ggatcacaac cttgccacct gataccgcaa acggataggc
    39361 ggtgttatcc gggttgttcg ggtcaaagac aaacaactgc gacgcagaaa ttgcaacctg
    39421 acttacaggc ctgccttcac tgtcttttcc ggcgacaatc ccgatccccg cagtgatacc
    39481 atcaactccc gctttttttg accacattgc cagaaacgcc tcaccgcctt ctttatccag
    39541 tttagtgatg cgcttgtcga cctcattaag cgattcactg gttgacgaat ccagtgtgct
    39601 aatgcgggtt tcaataccac caatcgttct tgtcgtttct tccctgagag tccccacaac
    39661 ttcggttgtc ttaattgcag catcctttac agcctgcccc tgcgcgtttt ttatttcttt
    39721 acgcagctcg gacacaaccg gcgactttgc agcctcatcg cggatctggt caatgatggc
    39781 cttcacgccg atctgtgttt gtgcctgagt gcctttttca gcattccatg gacctttcac
    39841 tcctgccgcg ttaacaaaac gtatccagta aaatcccgac cagccagggt caaccggatc
    39901 gccgtaaacc tgccccggcg tcgtggcaac cagcactgca tcagcaaggt catcctccgt
    39961 accccgccag atttcagtca gtgaatgtcc gcgataatta ggcatatccc attcaagaag
    40021 aaccgagcca aatcctcctg tcgccttaaa attcagcggt tttgtgggaa aatcaacagt
    40081 cattaaagta ctgtcaatct caatacccgg attcagtgca tatgaggcac cacccgatgt
    40141 tcgacgccgg gcgagtttaa gaccaaccag ttcctcacgg gtcacaaatg cgtggcgtcc
    40201 gtcaccacgc tgcccggtgc caatttccat gttctccaca actgtggata aatccttccc
    40261 cgcacgccac ggttttctgg tcataccggc atctccgaca tcgatgtact cagggttatt
    40321 cgttccacct gcccgaatcc ggataccatc acctgccagt tttgcccggt tgctgccgga
    40381 agtctcacca cacttccctt aaacgtaccc ggcgcaaaat taatcacagg aacatcatca
    40441 gccataatgg tgatccccac ccgctcaggc gccggagatt tcaccctgat acaggaaaaa
    40501 gaggttcttt caggtaatga aaaaattttt gaatgccacc ttatcgtgga gggcagagag
    40561 cccccggcaa gcactgacat tttgtctcct gtcaccacgc gcatcatatc tttcgcgaga
    40621 tcaacccatg cgcagtcaaa cggtgtactg agataacgga tatccatgtt caccggactg
    40681 aatacaaaca catcctgctt accatccggt ttcgtgtaac aggcaatgta ctcaccacgc
    40741 caggaataag ccacaatgga cgccgggtta aactgactct gccactgttc aggtgaaata
    40801 atcttttccg ttgccagtgc tgtattaccg tttacatcaa cagataccag accgtttgtc
    40861 ccggcataga gtacgaatcc ctccatcgcc accatacttc ttcggctcag gcatgcctgc
    40921 atggaaggaa ttctggagcc agaaattgtg gacggtgata ccccactgaa cagataaggc
    40981 tccccctttg tcgccaccac cagtgacgtt cccagcggac agatagccac aatatcttct
    41041 gccgtcgtgt gacgattcac ttccggccat gcatacggca gatacgcttc cgaaaacatc
    41101 acttcattac cggcaaaccc ggcggcaata ccattagcca tcaggcaaag gcctgtcata
    41161 ttctctggcg gcggcaggta atcccatgtc gccagagaag gtccaaggtt tttccccggt
    41221 attttgtccg tgtaactgag cacggatgca tccagttcag ccacaagtaa aaaatccgcc
    41281 tcccctccac ctgatgcaga gcgataaatc cggcggcgtt taatactggc attctgcaat
    41341 ggcaccggag acagcgtcag ctgtaccgca gtccccggag tacggagtgt tacctccaga
    41401 gacgccggac ctggcggacc ttcttcacca taatctgaga caaaggtttc cgtataaaac
    41461 cgggtttcgt catcattcgg gttatcgtca gaaacatcac cgccctgctg aacagtacag
    41521 acaggtgctg tcgtcggcgc ggggatcccc agacgatacg atgatgccgg atgattcccg
    41581 tccccttttg tggcaatggt tgcatccgtc actttaggaa aacgcccgtc agtgtagtaa
    41641 atacgcccgt gggggtcctg agcgatcgga ctgcggatca catccactac atccgtccat
    41701 gcaaaccaga aatcgtcacg gtaatgaaaa atggttttcg gcttaatcgc gaatgttttc
    41761 tcagcctctg acatctggtg ttcaggcgtg atcactccat agcgaaaatg acagttttct
    41821 gccagtacag cagaatgatc tggcagcata gatgcaataa cgcctggcat catcccgcgc
    41881 atagttgtta tatcgatata aggcatggtt gtctggtatc cgaaaatttg aatggcaggt
    41941 gagtcactga attgaagttt taaaggcggc atacaattgc gcctgtgcag aatacagatt
    42001 tccagaaatc ttgaaaaatt acatcattat tatcagcatc cgtccggaaa aatttctctg
    42061 gcaacttaaa acaatatcct atatatccgg cgtctccggt atgtagaacg tgaagttttt
    42121 aaaattattc atttaccatt taactaattt ttagatcctg ggttactccc gtctttttca
    42181 ttgcctccac gaaagcggaa ctaacagaac ctgaccagta atactggaac ccactaacaa
    42241 cgccctgata aatcatggtt tgtgtagcac cacctacgtt tacggtgatg ttacctgctt
    42301 taccaccgga taaataaaca gcagctgtcg gtgtagagtt cgtcaaaaag tgagaaagtg
    42361 taacgacagc ccccaccagt ctcccagaac cacagttttc agcgtttgta attgtccctt
    42421 tgcttttttc ttctaatggc caatcagctc caaccgcccc ccagttttca acttgatccg
    42481 aagagttcca tttatagtaa gcggtttcta ttttacaacc catagctcgc cccaccattt
    42541 gactcatcat aaaaggttta tccaatccta gtttagtgcc agcagccccc atccattgct
    42601 cacctgtttc gctcttagcc gaagaaccta cccatcccga tgttatcccc atttcatata
    42661 tccttctgaa attcgtgagg tgagtatcat ccccacaatt taaattcagt agaaaccaga
    42721 aactacaaca gctgtcgttt acttctcccg gagttcttta atctcatgac gaagtgtttt
    42781 aaagccttcg actaacaatg caattatgcc gttgtagtta agacgcagac gtttttcacc
    42841 agatataacg tctgcgtctt cagttaccag ttcaggcaag gctttttgtg catcctgagc
    42901 aattaaacca accgacgttt gccaaccgtc ggcagagtac tgtatctcgt aaagataacc
    42961 agtaagtgcc tccagacgat ctaacgcatt atccagtttt accagatttc gcttgttgcg
    43021 tttatccgag cggatctgaa catcgttaaa tgatccgttt ccgtttaccg tcaaatttcc
    43081 attaatacca ccattaaaag tttgtgcctg agtccatgta ttggcagtag taagcagttc
    43141 tgttccttgc ccgggggctc cagtgtctcc cttcggtccc tgcggccctt ccggacctgc
    43201 tggacctgct actcccggat cacctttatc gcctttcggc cccggcgcac ctgccggacc
    43261 tgctggtcca gccacccccg gatcgccttt gtcacctttt ggtccctgtg cgcctgccgg
    43321 gcctgcagct cccgtatccc ctttaggtcc ctgcggtcct gccgggcctg cggctcccgt
    43381 atccccttta ggtccctgcg gtcctgccgg gcctgcggct cccgtatccc ctttaggtcc
    43441 ctgtggcccg gttgcgccgg tggctccttt ctcacccttt gccccagcag cgccagtatc
    43501 cccctttggt ccctgggggc cgggatcacc tttcggtccc tgaacgcccc tcggcccagc
    43561 cggacctgct ggccccgagt cccctttatc tcctttcgga ccgggaacac cacctcctgc
    43621 tgcagcctct tctgcctttg ttttcgcttc atttgccaca tccattgccg ctttcaccgc
    43681 tttcggggtg gctgccttcg cttcatcatc actgtccgtt gcgctgctta actgcacaat
    43741 tcccttctgt gccgtcgtcg catcagccac atttgcagcg ctgcctgccg gacctggctc
    43801 tccacgaggt ccctgaggtc cggtctctcc tcgttcgcct ctcggacctg caggacctgg
    43861 ttcacctcgg gggccagtct ccccacgctc acctcgtgct cccatcggtc cctgtggtcc
    43921 ggcttctcct cgttcacctt taggaccttg cgggcctgca ggacctcccg gatcaccttt
    43981 ctcgcctttt ggccccatat ccccctggtc ccctttaggc ccccgctctc cggtatcccc
    44041 cttcaggcct ggtattccct gcggtcctcg ctccccctgt tcgcccttct caccacgcgg
    44101 accagcgggc cctacagccc cctgagcacc aacgtcacca cgctcacctt tcggccctgc
    44161 gggcccttga gggcccactg gacctgtttc gcctttagga ccgacatccc ccttcggacc
    44221 agtttctccc tgaggccccc ggggcccccg tgcattctca gccatacgtc tggcctcttc
    44281 agcactgaca gtggcagcct ctgcccgctt aaggatctct ccggcgctct cctgcgccag
    44341 cctggccttt tcagcatgct gtctggcttt ttctgcatca gctccggcgg ctttttcaga
    44401 ctctccggca cgggtcgagc tttcctctgc attccccgct gctgtgactg cacgggtcgc
    44461 agcctcagtg gcatcagtcg ctttttgtcc ggcttcagcc gccctgctgg ttgccgtctt
    44521 tgcactgtca gatgcactct tcgcactggc tgctgcactt tcttttgact gtgtggcctg
    44581 agtgtttttt gtcgccgtgt cttcattcag gcgacgaata ttggcaaggt catcagccac
    44641 attattctgt atctgccgga aatctgtcag cagttctccg ggtatgctaa cctcaacaag
    44701 actgcggcgt aacagcatat tgagcgtcac cgtactttcg gtcccctcaa tacgcacacg
    44761 tccgtagaca gcagtcttcc ctttcaccgt caccgaaacc gcatactccc ccggatccat
    44821 cgtcattccg taatatccac cttcacgggt cactgccgac gcactggtgc cgctgagcgc
    44881 atccggtgaa actgtcagcg ccgtcagggt aatatttgct cctgatatcg cctcaccatc
    44941 aggagatttc agcgtccccg aaacaacaac actcacactc cacctccgtt aaacactttt
    45001 ttacgggcag acaatgctct gtctgccccc tgtttgatcc caagttgctc aacaaaactc
    45061 tgataatgct gcgcagccag ccccgattct gcaccaccgg cagcatcctt actgaaagca
    45121 cgaaacaaca tccagtccac cagtgggtta acataagcct cttccagtgg aactggcgta
    45181 tcatcgtcct gcgtcagaac atacactgcc tccggtatcc ggcttaccac tgcatcaata
    45241 cttatctctt tgtcagggac aggaaacagc cagaatacgc gcggggacag gtcgttgctg
    45301 ataaaacatt caggaatgcc cttcactgtg ggccactcag gatactgcgc atccagcacc
    45361 tcccgggata atggtctgac tgcactaccg tcactgaggc atatcacgtc aagaagttgt
    45421 attacaccat cgggcaaaac ctgacgggcg ccaggaacac aactgattgt ttccaggctt
    45481 gcgccagcat ccggtctcgc cagaatcact gccctcacag catcattgta ataatcgcac
    45541 aattcctgca ggggccagcg aaccatcatc gggtcaacca gttgtgtatt cacacgtccg
    45601 atgatttctg taatcgtcgt catcagaaaa acctctgcct gcgtacaggg ttgcggtatg
    45661 aagagtacgg gcttgtcgcc agtgtatgac gatatgcccg acggatcccc tcagaaaact
    45721 gcacagaaaa atactgtgcg cgtaacggat ctgaccatga aacaccagtc tgcatgaaca
    45781 accgctcaag tgcccccgca gccacttctt caggccatgt gaggagttca tccggtatct
    45841 ggctgcgtcc ggctttcgga gcgacggcat aaagcacgct cacctcaccg ggagaacagg
    45901 caaatcgcag ggagcgtccg gagctgatat ccacatcccg accgacaaaa agctcatgat
    45961 tatcgtcaga gatacggatg atatgaacgc actcctcatc atctttgtca tacggaagca
    46021 cgatttcttt tcctgctact ggtacaacag taacctcccg acggcacacc aacgactggc
    46081 ggctgaatgc cacggcagcc attgacagag catccgtcat cataatgttc agtggaccgc
    46141 tgatatgacg acggacatac ggtaaaaaat cactcagttc cgccatgctg ttcagtctcc
    46201 gcaacacgac ggcgaaatgc ctcacgcacc cggatacgga atgcctcagc cgtttctttc
    46261 gggtctttgt gaatatccag ctcttctgcc tcacacagcg tcgccagccg tgctgaggtg
    46321 agcttactta aatccacctc ctgcccgtta acagaaacaa caaaactgtt ctccgcttct
    46381 gcccgcgcag caagcactct ttcctgcgcc tgctgtgcct gccgcaactg ctcattctgt
    46441 tgttgctttt tcagaacatc atcaagctct tcatgacgaa cccagacatc cggaaacccc
    46501 agcagttgcc aggccatcgc actgtcaaca tgcaccggct caagacgtgg gaacaatgtg
    46561 cggcttccgg taatggtgtc ctttttcacg ggttttgggc cgatatagac aacggcaatt
    46621 ttctcactca tataattccc cggataaaaa gcccgcatga cgcgggccgg aaggttttaa
    46681 tcagtatccc accacggtat aacgcagcag aacattcagg gtgccggttg cagcggcagt
    46741 cttaatggtg acagtaacca gctccccgtc acgctgtgtg gtgtacggct ccactggcac
    46801 atatctggca aattttgcag aaacagcttc gctgttatcg atgagagcat gctcaccgga
    46861 cttaatgctg acggttgcag tacccagacc acccgttgaa accagttgga gtgagttgat
    46921 acggatgccc actggcagtg agagaagatg aataacactg tccgcttccg cagcattcac
    46981 cgtaaatacg ccttctgcca ccgactcatt accgtgcgta cccgtataga cccgttcact
    47041 cagtgacggg gcaaggatag tctttgccat aattaatgac tcctgaaaaa gccgggcgaa
    47101 aacccggcat agggaaagga aaaaatcaga gcttcactgc tgtatcaacg gcaatcacgc
    47161 cgtgatcctg catcttgccg ctcttctcgg ggaaacggat ttttttcaga ccgttgatcc
    47221 agctgattgc tatctcagta cggttatcca tatccgtttt cttctcaacc atgttgaagt
    47281 gaccgcccgc cttctgaccg taagcatttg ccagcgcctg agcccccagt aacatggcgc
    47341 ggtcaatatt ggttgcagca gcgacctctt tcgtggttgc cgtcaggtta ttctctgata
    47401 ccagaacctt tgacccctga tagaaacgga tcggcatacc cgcatactta cgaaccagga
    47461 tattgcgcca catcgcacat tcacctttga acagcggatg attaaaacct tttgcacggt
    47521 tcacggcacg aaccatcatc tggttccagt ccttaccgga cgtcgaggtg taccagtcat
    47581 tccactgacg cggcgtgacg tacaggacgt aatatggatc ttctccgtga agttcatcac
    47641 cggacagacg aaccggctgt aacggatgcg ccatttcgtc aatgaacagg gagagattgt
    47701 ccaccaggcc aatagaaaaa atatctgccg cttcaatctg ctcaaagctt gtcgcatcac
    47761 cgccaaaaaa gtgacggtca tgtgtcggag gcagtacatc gttgatcatg atttttttga
    47821 attcagggtg ctccgctgtc ggcagaatag tgtcgtcagc aacaaaatca ccacgagctc
    47881 cagcaagatg cactatcgca cactggtcct gcaggtcatt aaagtacgtc cccagaagcg
    47941 ttctggctga ggatgccaag ttaaacttcg tgcgctgctg actcatacgt ccgcctgcat
    48001 ccaccaggtg acgtccctga ttgattttca gggagaagtc agcatggctg agatcctcac
    48061 cacgaccttc aacacgctca tctcccatcg tcgggcgttt tgagagtttg tgcatgatgc
    48121 tgaaggtcac ttcatcaccg gcctgtttgt taaggtctgt gatacggaca accggcgcac
    48181 ccgcgctggt ctgcttcgtg cttttcttgt ccggcgaaac cgcttttggc gcttcctgct
    48241 gttcagtgag gatattgacc atcgagcggt tgcggttggc agcggtaaaa agcgccacct
    48301 gatacagctt attcgcctgg gctgatgtta cagtcgtcat tacttcagtt ctccttcagt
    48361 aagttacccg agcttctcca gaagcgcgtc tatttcagca ttcgtcatac cgcgcataat
    48421 cgcctctgcc tctgaatgag aagcgccaag taaccgttca aaattatcac cggttccgac
    48481 ggaagccgtg gtgcctaaat ctgacgggga agcaggtact gcctgctcct gttcagcggt
    48541 cttcactttc tcttccgccg ttttccggat atccgttttg tctgccttgt tgtcagcaga
    48601 cgactcactg acttcaccga aagcaacctg cgtacgacgg gccacttcag cgaaacgttc
    48661 agtgagcgtt ttgtctttcc atgcggggtc attctggagc ttcccgtcga tggatacagc
    48721 aaccgagaag cgatctggat cggactcctg ccacgttttc agcaccggca cggcattcat
    48781 cgcatcaaga accggtgata aatcctcacc accattacct tctgcctgct gtgctgattg
    48841 ctgaacacgg gactggagat agttattttt acggatgagc gaagccaccg cgtcaccaat
    48901 ttccggatac atctccctga tacgggcaat ctgctcatca gaaatttttt cgttttccgg
    48961 taacggtgtg ggcttcatac cggcctggtg gatctgagac gtcagcagtt ccaccctgcg
    49021 tttttcttca gctatctgcc cacgaagaag tgcggcttcc tgttcggccc gttgcttacc
    49081 ggaacgttca gcctcaagga cttcataggg aatgacgtgt ttaccgtcgc gggtgagcac
    49141 ccccttcgct tccggctcct tcacgtcctg cgtctgctcc acactggcat ccggcgtcgg
    49201 tgccacattg ttatcgcccg tctgagtctg tgcttcctca tccgcatgtt tttccgtggt
    49261 atcttccgtc acgacgtcct gtgcgtgact gtcaatatcc acatccccaa gtccttccag
    49321 cattttttcc agttgttccg gggtttcttc acccgtaaat tcaaaatcca taaataactc
    49381 cgcatggtct gtttatcgga cagatccgaa tggttgagta aataaggctt atcgctgccc
    49441 ccgcgaataa gcgcaccgct cccggaacgc ttacctccgg aaacaaaaaa ccccgtacga
    49501 tgacggggtt cagttgaagc cagagttttc agagcgacat ttcattcatc cgctgttgta
    49561 acgtatacag catctgttgc tgaagaacgt cctgctcctg ttccatattc tgtacgccgg
    49621 taatgatttc tgccgtatgt gcctggttaa gcgcatccac ataacgctgc ccctgtgtca
    49681 gggcgacttc ccgctgtgca ctggcattat cccgttgtgc agctgcatgt gccctggcgg
    49741 cgtcagcttc cagttttgcc actctgccag ccatctcgcg catctggagt tctgcctgtt
    49801 gttgctgaag tgcctgttgt tgtgccgcta cttcctgttc ttccggcgtc atttcatccg
    49861 gtgattttgg cgtccccagc gcagcacgaa tacgctcaac aaactcctgt ttctgcggca
    49921 catccagaag attaacccac aggtcgagca caacagcctg cacctgaggc ggcagcccct
    49981 gaataacctc tgacattctc tgtgcaagct gtgccttaaa cgccggtgtc tgctgaacag
    50041 gcgccagcgc aatatgtgta tttaaccttg aaatatcatt ggtcagttca ccattatcac
    50101 cttcagcatt gaggacaatg gtctggcgac gctggcgatc atcgcgatta atcaccactg
    50161 catgattacg gcgttttttc aggtcatcga gaagataagc cagcaacagt cttcccacct
    50221 gctggcaggc aaactggtag ttatcgttga tttccgcaag ggttgtggcc ccctgctcca
    50281 ccaggttact gatagccacg cctgacgtcg cacctgaatc ctgcccgaga aatgcggaat
    50341 acactcccat ggtatcctgg ataagttttt ccgattcctg catgacctga aactgctggc
    50401 tggcaacctg aaaatcctgc tcaacccgaa aaacatctgc gacacttttc tgattttttc
    50461 ggaccggatt cagtttaata atgccatccg gacgttcgat ctgctccatc aggtcgttgt
    50521 ctgacaactg ggtggcatcc tcgtccataa tcacgcgttt ggcctgaagc aaccaggtca
    50581 gcttgatacg acgaaaattc acctcatcct gtgccggaat ggcgcgggaa attagcccgt
    50641 atggctcccc ggttttatcc tttcggtatc cccagaaagg aaccagcgga aacatcccct
    50701 gcggagcact acaggggcga tccacaataa agtgcggccc gacaaaccag gcttcacgaa
    50761 tacggcttac ccgcccgact ttcacctgaa cccgcccgga tgccacagct accgcctgca
    50821 tcagattatt tttatcaaag gccaccaccc gtccattact gagttcaatc accggaagac
    50881 gctcgaatgt acggtaataa accacctgaa gcagcacacg acggcgttca cgctgaagcc
    50941 attcgttctg ctgtcgatcc catgactgat actcttccca tgcactcatc aacggactgg
    51001 gctggccttc agtaaccgtg gtatcgacaa aaccacgcca gtcatcaatg gcataatcga
    51061 taacctgagc cattcccggg aatgtagctt ttgcctcatc ggtatccatc cagcggcgac
    51121 gcatcagcca gcggcagtca cttaaatcag cctcccggct cagccagtcc cagaaaacct
    51181 cattccggct gacagtagac accttaaatt caggcccgaa cggatcactg tttcgtctga
    51241 cctccaccca actgaggccc gccttgattt gttccgcata ggcatcggag cgggccttat
    51301 tcatgttgcc aagacggcac gcatcagcaa actccgcatt aatggcctct gccagtttct
    51361 ctgtttcatc gttcggatcg tctgacatca ctatcaggtc cgttcttgtt tttgcctcca
    51421 ttcccagtac accatctacc gtgggggcaa tgaggttatg gatggtcatg ggctgaccgc
    51481 gatctttcag tacctggata acttccggtg ccagctggtc gccatcataa tacgcacagg
    51541 ccttgtttgc ggcatcacgc cagagaggct gactgtcaat atcagaacag agagacagta
    51601 actgacgctg agaaaaacgc ggcgtggatc catgatcgtt tttcatcgct gtggtgttaa
    51661 tttcattttt catcagtgtg ccatccagtg tgtggttctg cgtttatccg ttttctgttt
    51721 taccctcacc ggcattctgg cgcgcatctc ctgggcaatc atgtagctca tgagctgatc
    51781 atcaaagcag ccttcctgtg cattcatgga gcctttcgcg tcataaacgt aggtgttcat
    51841 ttccgataat gtgcctgacc agcggatccc tgatattcca ttattcagaa gcgttttcat
    51901 tccttcggtc agaacaggtt tgctctgacg ggttgtcagc cagccaaggc ggggcgtatc
    51961 gtcgtcatat gcctggtcaa gatgctgttc gttgtagata taacgtgtcg gatagagttc
    52021 ccggagtttc aggataactg catgtccgtg attattacgc tccggcccca caaacgcgtt
    52081 gttatacata cgacagacct gcgaaatgag atgagcaaaa agttcagcat cgagatgccc
    52141 gaaccagtga gccacctgct cgccattact gcgtttgaca acatccagcg atgagcggtc
    52201 tccgtgctcc agcccttcgg cagtatctgc cccacaaaca tactcttcat ccggatccgg
    52261 cagttcccat accagcagat aattcatcag cgtccgctgc aactcgtttt tatttccttc
    52321 acgcagagac tgagctttag tcttcgctcc tgtaacaggt tcaatgtcat aaacaatcat
    52381 cggtggcgaa cagaatgatt ctgcctgcaa cgtactttcg gcactgaaca cacgtcgtcc
    52441 ggacgtcaga aacgcctcct gtggcgttga gggaaactcc tgcttcattt cctcacgctg
    52501 ttcagtttcc ttattgatgt accactgctt ctgttcatca gtaagcgtga tgttcattgc
    52561 cttctcaacc gcagaaaaat acgtcatttt ttcccgtgac agcttcagcc cgctttccgg
    52621 cactctggcg ctgtatttag gatcctgcca ccaggcgtaa aaatggaatt tataatcctg
    52681 tgccgtcagc aataagcctg atgcagtgat ctcctgtgct cggttactca tctcgtaaaa
    52741 atcaccaccc acgccttcag cagtggactc atcaaaaata atgcattcat cagagacggc
    52801 attaagcgta ccggttcgca gttctttcgc cttagccgga tatttcgcgc aaattttgcc
    52861 gtgctctgag atatgcaggc gctgcaccgt acctgaacgg aatgaggttg ccacctggat
    52921 actcgagccg tgaccaaaca ggatatagcc accgctggca ccgctacgac gttcaacgat
    52981 ggtgaatgag gctctcagcc agtcagggag atgatcaaac ggtacagcaa tttttgtgcg
    53041 gaaaatttca ctggcagcct gtttatcctg agcgacgatc ccgcatttga gatgcggaat
    53101 gaataatgcc tggtcgagaa gataaatatc aatggctgtg gaaaatccca gctggcgcgc
    53161 tttcaggata atatttttat tgtgcatgct ccggaacaac tggcgctgcg ccggtcgcat
    53221 tctgaaggtg accagttcac ctttttcgtt ctgtattttg tagagatgat tgagccgcca
    53281 ccagggattg ctcagtttag tcatgatgaa cagacgttgt tcagcctcgg tcatttctga
    53341 cggctcatca catcgcggtt cattcttccg gaatgtcatc cagtctcccc gaattactca
    53401 tttcatgcag cgatgacacg atgtcactga caggcgtaac aacgccccga cgctggctgg
    53461 tcagaatatc ggtttccgct ctgagtttat ctctggcggc gttgattctt tcccggtcag
    53521 cacgaagttt tggtgctgtc tcagccagga cgtccagcgt cagcaatgag cgttcaattg
    53581 actcgatacg ggcaatattc cggtcaaggg cctgttcagc tttgagtatt ttgtcgtaaa
    53641 gagcaacgcg ggtttccacg tcagttgcct cttccaggtc ggcgaacatc cctttaagtg
    53701 ccttagttac tgaaagtgcg cgggcccggg tgaacaccag ttcatcgaac agcaccatgt
    53761 cggacgcatc atccatgagg ttatctgcct caagatactt cgcatatcca cggtgtctta
    53821 cggcgtgggt gtttcgctga gaaaaagcgt ttgaaggtgg taaaagtcgg gaaccacgaa
    53881 tccgtttcgt ttctgccgaa tttgcgcagt ttttttcaga gttttttgcg catttttcat
    53941 cgccggaacc cgcgtcattg cagggttctt catctgagat gtcatgatcg atttcatgat
    54001 cggttttatg atcaatttca tgatcgattt tgcccatttt tatacgggtt ctggcggtgt
    54061 tgtaattaat ctttttcttc cggcaccagt ccagtaatgt tattcccgtt tcggcatgtt
    54121 cgcgtcggaa tgcctgctcc agctttttcc agtccagctt tgccatgtca ctttctgacg
    54181 tcctgtgtta aaaactgatg cataatgacc gctgtgattt ttcagatttc acacagcagc
    54241 accatatttg atcgatattt gcacaatgcg gttgttttat ccggtttctt ccaccaccgc
    54301 accggacagg cggcttcgcg ggaaatcgct cccatctcgt gaaaaatgag aaaacccggt
    54361 gtgcatcgtt tttgattatc cccgcacact cacgcagata aggtggctgc agtctctgtt
    54421 aatgcgggaa tacggcgacg atacggcgca tggctatgtc aggctgaaat gcctttatca
    54481 aatccgggta acgcaatctg cccctgctgc tccagcctgt ccagccttgc cagcaactga
    54541 ggcttcttca ccctgcccca gcgattgagc aaacgacctg acatactggc gacatctttc
    54601 tctttcatgt actccagcat tacagcgttg cgctccgcct ccagattcgc cagaccttgc
    54661 tgaataagct cagccatcca gttgaaagcc tgaatgtacg cttccttaaa ggtcatagca
    54721 gccttccccg taaaaccaaa taccaacatc gtccaaccgt cttttgtcat tcgatatatg
    54781 ggctggggtt taccgttctg taactcattg ttttcatagc aaagcgcaaa attgcgctct
    54841 gcaaattctt ttgagcattg ttgaacaacg gctcttgttt tccgcaatac atctttgtgt
    54901 tctttgccaa atgctacggc aacctttcgg gtatccgtca ccggctcgtt gcctgtcaca
    54961 aaaacaagat ctcgaaaatc gatgccgtta acgatagttg gataattcat cagtgcacac
    55021 cttttagtga tgaaccttgt cacacaggat tccggcccac agaaaggcac cgatcaccaa
    55081 accggcatcc tcaagggtca tcctgaaagg ttctgtgttc ataagtcgcg cgtgtgaagc
    55141 gcgtttactg cggacataaa aaagccccgt atcgcgaggc tcattaaatt gactttgtga
    55201 tttgcaaaaa aattatttca ggcattgcgt cctgatgtat tcctgcaggt agttaacctg
    55261 cgcagttatc ttgtcgattc cacttctgag acggtaataa ttgagttcag catctgctgt
    55321 aagtcctggg ctttctccat cgcccatgct gctggctccg gtcgttgact ttgcacaggt
    55381 ggcggcgact tgcaggcgct tacgcccagc agaaacatca gcacggagac tttcgatagt
    55441 cgcattagca tcagcaagct cctttgtgta tctggcgtca agttctgcta catcacgttg
    55501 ccgcttctgc atatctgcga tgatggatgt ggctttatcg cgctgctctt tgtaagcgat
    55561 ggcgttatca cggtaatgat tcagccccag actaagcgca ccacagacca ccagcagaat
    55621 aacggtaaac gcggaaagca ttcggtttat gctcacccca ccagccctgc cgaagtcaac
    55681 gccatccagt ttatggaaag aaaaagaaca accagcatta gtgaaaatga aataccgacg
    55741 attacacaaa ggcccttcgc cagcgttatg agtttatccg atatcattag ccaccactcc
    55801 atcaatccgc ctttgttatt ttccctttgc ctgtatcagc caggacaaaa tcaatcagca
    55861 gattcgcttc gtttatcaat gtgcggattt ttgatacatg cgcggcttta acctgtttcc
    55921 actcattcag cccggtagca aacacactgg caatgttttt atcccgtttc atgtcagcgc
    55981 aggcctgatt gagttcttcc atcacactca ttcgacgggg attaacgaca ccccccttcg
    56041 tccagtattc atagagaaca tcgtcgcact cttcctgata ctggattacc ttgtcgcgga
    56101 tttcgggttt tactttgttg ggattgatgg tttgtaacca gcctgcaagt tttcgaagtg
    56161 gcagggacac catattgcgt cgtttcccat cctcagcaac cataacgatt tccgttatag
    56221 ttgacgcaaa acgctgtctt aacttagcca actgtgattg ccaggccagc cccatccccg
    56281 caacgacagg tttcatggga acgtatggct caccgttatg gttaaccaca taaagagaat
    56341 cgccgtgaaa cggcacggtc atcatattca tcggttattt ccttttagtg atgaaccctg
    56401 cgcacaggaa taaccagccc aaagagggtt aaccagacca ctgccggtta tccaccaggg
    56461 ctcatcctga aaggttcttt ggtttattta cgcttgtgcg aagcgcagaa atgacaaaga
    56521 caccattacg gtgcctcagc gtgaaacaat cttcctgact ttattcactt gcattttgcc
    56581 agttcgcagg atttcgtgtt atccgtccgc gtgagcaaac gtcatttttc agcaaaatat
    56641 tctgctacct gtcgataccc cagcatgcca gcgcactctc ctggtcgcga cgggatacct
    56701 gaccgtagca attatttgag cggatacggc agtctctgcc accgtcctta atccaccagc
    56761 gaatcgcttc acacgctccc ctgcgatcgc ctgcattaat tcgtttataa aacgtcgacg
    56821 ggaaacactt accggggcca atgttgtaag gacagaatga cgcgatcccc gctttctgag
    56881 gttcggtcag tggcactctg atgtttttcg ccacccatgc cagcgcctta tcacgttcaa
    56941 tggcgttaac ctggtcgcat ttttccttcg acaacttcat gccggggacg acaagcttac
    57001 catccacctg ggtggcacca cggcagatgg tccagatacc c
//
LOCUS       DAZKHC020000019        55941 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZKHC020000019 DAZKHC020000000
VERSION     DAZKHC020000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 55941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.019"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            180..416
                     /gene="lpp"
                     /locus_tag="ACHOSM_RS06955"
                     /old_locus_tag="pgaptmp_001391"
     CDS             180..416
                     /gene="lpp"
                     /locus_tag="ACHOSM_RS06955"
                     /old_locus_tag="pgaptmp_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HGU4557948.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(480..1484)
                     /gene="ldtE"
                     /locus_tag="ACHOSM_RS06960"
                     /old_locus_tag="pgaptmp_001392"
     CDS             complement(480..1484)
                     /gene="ldtE"
                     /locus_tag="ACHOSM_RS06960"
                     /old_locus_tag="pgaptmp_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HGU4557949.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTCTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(1633..2049)
                     /gene="sufE"
                     /locus_tag="ACHOSM_RS06965"
                     /old_locus_tag="pgaptmp_001393"
     CDS             complement(1633..2049)
                     /gene="sufE"
                     /locus_tag="ACHOSM_RS06965"
                     /old_locus_tag="pgaptmp_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HGU4557950.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFKKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(2062..3282)
                     /gene="sufS"
                     /locus_tag="ACHOSM_RS06970"
                     /old_locus_tag="pgaptmp_001394"
     CDS             complement(2062..3282)
                     /gene="sufS"
                     /locus_tag="ACHOSM_RS06970"
                     /old_locus_tag="pgaptmp_001394"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HGU4557951.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAGMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVSDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(3279..4550)
                     /gene="sufD"
                     /locus_tag="ACHOSM_RS06975"
                     /old_locus_tag="pgaptmp_001395"
     CDS             complement(3279..4550)
                     /gene="sufD"
                     /locus_tag="ACHOSM_RS06975"
                     /old_locus_tag="pgaptmp_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGU4557952.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMYITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(4525..5271)
                     /gene="sufC"
                     /locus_tag="ACHOSM_RS06980"
                     /old_locus_tag="pgaptmp_001396"
     CDS             complement(4525..5271)
                     /gene="sufC"
                     /locus_tag="ACHOSM_RS06980"
                     /old_locus_tag="pgaptmp_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGU4557953.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(5281..6768)
                     /gene="sufB"
                     /locus_tag="ACHOSM_RS06985"
                     /old_locus_tag="pgaptmp_001397"
     CDS             complement(5281..6768)
                     /gene="sufB"
                     /locus_tag="ACHOSM_RS06985"
                     /old_locus_tag="pgaptmp_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGU4557954.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLKAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(6777..7145)
                     /gene="sufA"
                     /locus_tag="ACHOSM_RS06990"
                     /old_locus_tag="pgaptmp_001398"
     CDS             complement(6777..7145)
                     /gene="sufA"
                     /locus_tag="ACHOSM_RS06990"
                     /old_locus_tag="pgaptmp_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HGU4557955.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(7693..7881)
                     /gene="ydiH"
                     /locus_tag="ACHOSM_RS06995"
                     /old_locus_tag="pgaptmp_001399"
     CDS             complement(7693..7881)
                     /gene="ydiH"
                     /locus_tag="ACHOSM_RS06995"
                     /old_locus_tag="pgaptmp_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HGU4557956.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(7981..8391)
                     /gene="menI"
                     /locus_tag="ACHOSM_RS07000"
                     /old_locus_tag="pgaptmp_001400"
     CDS             complement(7981..8391)
                     /gene="menI"
                     /locus_tag="ACHOSM_RS07000"
                     /old_locus_tag="pgaptmp_001400"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HGU4557957.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(8388..11444)
                     /gene="ydiJ"
                     /locus_tag="ACHOSM_RS07005"
                     /old_locus_tag="pgaptmp_001401"
     CDS             complement(8388..11444)
                     /gene="ydiJ"
                     /locus_tag="ACHOSM_RS07005"
                     /old_locus_tag="pgaptmp_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HGU4557958.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEITK"
     gene            11833..12945
                     /gene="ydiK"
                     /locus_tag="ACHOSM_RS07010"
                     /old_locus_tag="pgaptmp_001402"
     CDS             11833..12945
                     /gene="ydiK"
                     /locus_tag="ACHOSM_RS07010"
                     /old_locus_tag="pgaptmp_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HGU4557959.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            13131..13238
                     /gene="rprA"
                     /locus_tag="ACHOSM_RS07015"
                     /old_locus_tag="pgaptmp_001403"
     ncRNA           13131..13238
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHOSM_RS07015"
                     /old_locus_tag="pgaptmp_001403"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            13374..13730
                     /gene="ydiL"
                     /locus_tag="ACHOSM_RS07020"
                     /old_locus_tag="pgaptmp_001404"
     CDS             13374..13730
                     /gene="ydiL"
                     /locus_tag="ACHOSM_RS07020"
                     /old_locus_tag="pgaptmp_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HGU4557960.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            13830..15044
                     /gene="ydiM"
                     /locus_tag="ACHOSM_RS07025"
                     /old_locus_tag="pgaptmp_001405"
     CDS             13830..15044
                     /gene="ydiM"
                     /locus_tag="ACHOSM_RS07025"
                     /old_locus_tag="pgaptmp_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4557961.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            15266..16531
                     /gene="ydiN"
                     /locus_tag="ACHOSM_RS07030"
                     /old_locus_tag="pgaptmp_001406"
     CDS             15266..16531
                     /gene="ydiN"
                     /locus_tag="ACHOSM_RS07030"
                     /old_locus_tag="pgaptmp_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4557962.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLYTIKHRG"
     gene            16543..17409
                     /gene="ydiB"
                     /locus_tag="ACHOSM_RS07035"
                     /old_locus_tag="pgaptmp_001407"
     CDS             16543..17409
                     /gene="ydiB"
                     /locus_tag="ACHOSM_RS07035"
                     /old_locus_tag="pgaptmp_001407"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HGU4557963.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVY
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            17440..18198
                     /gene="aroD"
                     /locus_tag="ACHOSM_RS07040"
                     /old_locus_tag="pgaptmp_001408"
     CDS             17440..18198
                     /gene="aroD"
                     /locus_tag="ACHOSM_RS07040"
                     /old_locus_tag="pgaptmp_001408"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGU4557964.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVACAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            18349..19944
                     /gene="ydiF"
                     /locus_tag="ACHOSM_RS07045"
                     /old_locus_tag="pgaptmp_001409"
     CDS             18349..19944
                     /gene="ydiF"
                     /locus_tag="ACHOSM_RS07045"
                     /old_locus_tag="pgaptmp_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HGU4557965.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVYNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAEGNVGVGIADGIGLVAREEGCADDFILTVET
                     APIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            19958..21109
                     /gene="ydiO"
                     /locus_tag="ACHOSM_RS07050"
                     /old_locus_tag="pgaptmp_001410"
     CDS             19958..21109
                     /gene="ydiO"
                     /locus_tag="ACHOSM_RS07050"
                     /old_locus_tag="pgaptmp_001410"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HGU4557966.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(21152..22063)
                     /gene="ydiP"
                     /locus_tag="ACHOSM_RS07055"
                     /old_locus_tag="pgaptmp_001411"
     CDS             complement(21152..22063)
                     /gene="ydiP"
                     /locus_tag="ACHOSM_RS07055"
                     /old_locus_tag="pgaptmp_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4557967.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVLKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSELGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            22379..23143
                     /gene="ydiQ"
                     /locus_tag="ACHOSM_RS07060"
                     /old_locus_tag="pgaptmp_001412"
     CDS             22379..23143
                     /gene="ydiQ"
                     /locus_tag="ACHOSM_RS07060"
                     /old_locus_tag="pgaptmp_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HGU4557968.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            23163..24101
                     /gene="ydiR"
                     /locus_tag="ACHOSM_RS07065"
                     /old_locus_tag="pgaptmp_001413"
     CDS             23163..24101
                     /gene="ydiR"
                     /locus_tag="ACHOSM_RS07065"
                     /old_locus_tag="pgaptmp_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HGU4557969.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            24157..25446
                     /gene="ydiS"
                     /locus_tag="ACHOSM_RS07070"
                     /old_locus_tag="pgaptmp_001414"
     CDS             24157..25446
                     /gene="ydiS"
                     /locus_tag="ACHOSM_RS07070"
                     /old_locus_tag="pgaptmp_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4557970.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILQANVVVLAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KKREDFSASGLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            25443..25736
                     /gene="ydiT"
                     /locus_tag="ACHOSM_RS07075"
                     /old_locus_tag="pgaptmp_001415"
     CDS             25443..25736
                     /gene="ydiT"
                     /locus_tag="ACHOSM_RS07075"
                     /old_locus_tag="pgaptmp_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU4557971.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            25793..27439
                     /gene="fadK"
                     /locus_tag="ACHOSM_RS07080"
                     /old_locus_tag="pgaptmp_001416"
     CDS             25793..27439
                     /gene="fadK"
                     /locus_tag="ACHOSM_RS07080"
                     /old_locus_tag="pgaptmp_001416"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HGU4557972.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHVTGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(27496..29874)
                     /gene="ppsA"
                     /locus_tag="ACHOSM_RS07085"
                     /old_locus_tag="pgaptmp_001417"
     CDS             complement(27496..29874)
                     /gene="ppsA"
                     /locus_tag="ACHOSM_RS07085"
                     /old_locus_tag="pgaptmp_001417"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HGU4557973.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AQKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            30207..31040
                     /gene="ppsR"
                     /locus_tag="ACHOSM_RS07090"
                     /old_locus_tag="pgaptmp_001418"
     CDS             30207..31040
                     /gene="ppsR"
                     /locus_tag="ACHOSM_RS07090"
                     /old_locus_tag="pgaptmp_001418"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HGU4557974.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            31197..32243
                     /gene="aroH"
                     /locus_tag="ACHOSM_RS07095"
                     /old_locus_tag="pgaptmp_001419"
     CDS             31197..32243
                     /gene="aroH"
                     /locus_tag="ACHOSM_RS07095"
                     /old_locus_tag="pgaptmp_001419"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HGU4557975.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            32375..32566
                     /gene="hemP"
                     /locus_tag="ACHOSM_RS07100"
                     /old_locus_tag="pgaptmp_001420"
     CDS             32375..32566
                     /gene="hemP"
                     /locus_tag="ACHOSM_RS07100"
                     /old_locus_tag="pgaptmp_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HGU4557976.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(32570..34006)
                     /gene="selO"
                     /locus_tag="ACHOSM_RS07105"
                     /old_locus_tag="pgaptmp_001421"
     CDS             complement(32570..34006)
                     /gene="selO"
                     /locus_tag="ACHOSM_RS07105"
                     /old_locus_tag="pgaptmp_001421"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HGU4557977.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLIRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLADDEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(34069..34782)
                     /gene="ydiV"
                     /locus_tag="ACHOSM_RS07110"
                     /old_locus_tag="pgaptmp_001422"
     CDS             complement(34069..34782)
                     /gene="ydiV"
                     /locus_tag="ACHOSM_RS07110"
                     /old_locus_tag="pgaptmp_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HGU4557978.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            complement(35029..35493)
                     /gene="nlpC"
                     /locus_tag="ACHOSM_RS07115"
                     /old_locus_tag="pgaptmp_001423"
     CDS             complement(35029..35493)
                     /gene="nlpC"
                     /locus_tag="ACHOSM_RS07115"
                     /old_locus_tag="pgaptmp_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU4557979.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(35571..36320)
                     /gene="btuD"
                     /locus_tag="ACHOSM_RS07120"
                     /old_locus_tag="pgaptmp_001424"
     CDS             complement(35571..36320)
                     /gene="btuD"
                     /locus_tag="ACHOSM_RS07120"
                     /old_locus_tag="pgaptmp_001424"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HGU4557980.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(36320..36871)
                     /gene="btuE"
                     /locus_tag="ACHOSM_RS07125"
                     /old_locus_tag="pgaptmp_001425"
     CDS             complement(36320..36871)
                     /gene="btuE"
                     /locus_tag="ACHOSM_RS07125"
                     /old_locus_tag="pgaptmp_001425"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HGU4557981.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(36934..37914)
                     /gene="btuC"
                     /locus_tag="ACHOSM_RS07130"
                     /old_locus_tag="pgaptmp_001426"
     CDS             complement(36934..37914)
                     /gene="btuC"
                     /locus_tag="ACHOSM_RS07130"
                     /old_locus_tag="pgaptmp_001426"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HGU4557982.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLVLGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            complement(38015..38314)
                     /gene="ihfA"
                     /locus_tag="ACHOSM_RS07135"
                     /old_locus_tag="pgaptmp_001427"
     CDS             complement(38015..38314)
                     /gene="ihfA"
                     /locus_tag="ACHOSM_RS07135"
                     /old_locus_tag="pgaptmp_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HGU4557983.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(38319..40706)
                     /gene="pheT"
                     /locus_tag="ACHOSM_RS07140"
                     /old_locus_tag="pgaptmp_001428"
     CDS             complement(38319..40706)
                     /gene="pheT"
                     /locus_tag="ACHOSM_RS07140"
                     /old_locus_tag="pgaptmp_001428"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGU4557984.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(40721..41704)
                     /gene="pheS"
                     /locus_tag="ACHOSM_RS07145"
                     /old_locus_tag="pgaptmp_001429"
     CDS             complement(40721..41704)
                     /gene="pheS"
                     /locus_tag="ACHOSM_RS07145"
                     /old_locus_tag="pgaptmp_001429"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGU4557985.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(41913..42037)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(41987..42031)
                     /gene="pheM"
                     /locus_tag="ACHOSM_RS07150"
                     /old_locus_tag="pgaptmp_001430"
     CDS             complement(41987..42031)
                     /gene="pheM"
                     /locus_tag="ACHOSM_RS07150"
                     /old_locus_tag="pgaptmp_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HGU4557986.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(42154..42510)
                     /gene="rplT"
                     /locus_tag="ACHOSM_RS07155"
                     /old_locus_tag="pgaptmp_001431"
     CDS             complement(42154..42510)
                     /gene="rplT"
                     /locus_tag="ACHOSM_RS07155"
                     /old_locus_tag="pgaptmp_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGU4557987.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(42563..42760)
                     /gene="rpmI"
                     /locus_tag="ACHOSM_RS07160"
                     /old_locus_tag="pgaptmp_001432"
     CDS             complement(42563..42760)
                     /gene="rpmI"
                     /locus_tag="ACHOSM_RS07160"
                     /old_locus_tag="pgaptmp_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGU4557988.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(42857..43399)
                     /gene="infC"
                     /locus_tag="ACHOSM_RS07165"
                     /old_locus_tag="pgaptmp_001433"
     CDS             complement(42857..43399)
                     /gene="infC"
                     /locus_tag="ACHOSM_RS07165"
                     /old_locus_tag="pgaptmp_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGU4557989.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(43403..45331)
                     /gene="thrS"
                     /locus_tag="ACHOSM_RS07170"
                     /old_locus_tag="pgaptmp_001434"
     CDS             complement(43403..45331)
                     /gene="thrS"
                     /locus_tag="ACHOSM_RS07170"
                     /old_locus_tag="pgaptmp_001434"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGU4557990.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            45855..47752
                     /gene="espL1"
                     /locus_tag="ACHOSM_RS07175"
                     /old_locus_tag="pgaptmp_001435"
                     /pseudo
     CDS             45855..47752
                     /gene="espL1"
                     /locus_tag="ACHOSM_RS07175"
                     /old_locus_tag="pgaptmp_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            47924..48031
                     /gene="yniD"
                     /locus_tag="ACHOSM_RS07180"
                     /old_locus_tag="pgaptmp_001436"
     CDS             47924..48031
                     /gene="yniD"
                     /locus_tag="ACHOSM_RS07180"
                     /old_locus_tag="pgaptmp_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HGU4557991.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(48084..48842)
                     /gene="ydiY"
                     /locus_tag="ACHOSM_RS07185"
                     /old_locus_tag="pgaptmp_001437"
     CDS             complement(48084..48842)
                     /gene="ydiY"
                     /locus_tag="ACHOSM_RS07185"
                     /old_locus_tag="pgaptmp_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HGU4557992.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            49130..50059
                     /gene="pfkB"
                     /locus_tag="ACHOSM_RS07190"
                     /old_locus_tag="pgaptmp_001438"
     CDS             49130..50059
                     /gene="pfkB"
                     /locus_tag="ACHOSM_RS07190"
                     /old_locus_tag="pgaptmp_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HGU4557993.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            50160..50450
                     /gene="ghoS"
                     /locus_tag="ACHOSM_RS07195"
                     /old_locus_tag="pgaptmp_001439"
     CDS             50160..50450
                     /gene="ghoS"
                     /locus_tag="ACHOSM_RS07195"
                     /old_locus_tag="pgaptmp_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU4557994.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNSK"
     gene            50556..51416
                     /gene="yniA"
                     /locus_tag="ACHOSM_RS07200"
                     /old_locus_tag="pgaptmp_001440"
     CDS             50556..51416
                     /gene="yniA"
                     /locus_tag="ACHOSM_RS07200"
                     /old_locus_tag="pgaptmp_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HGU4557995.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(51457..51993)
                     /gene="yniB"
                     /locus_tag="ACHOSM_RS07205"
                     /old_locus_tag="pgaptmp_001441"
     CDS             complement(51457..51993)
                     /gene="yniB"
                     /locus_tag="ACHOSM_RS07205"
                     /old_locus_tag="pgaptmp_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HGU4557996.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPIFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            52140..52808
                     /gene="hxpB"
                     /locus_tag="ACHOSM_RS07210"
                     /old_locus_tag="pgaptmp_001442"
     CDS             52140..52808
                     /gene="hxpB"
                     /locus_tag="ACHOSM_RS07210"
                     /old_locus_tag="pgaptmp_001442"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HGU4557997.1"
                     /translation="MSTPRQILAAIFDVDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            52971..53561
                     /gene="ydjM"
                     /locus_tag="ACHOSM_RS07215"
                     /old_locus_tag="pgaptmp_001443"
     CDS             52971..53561
                     /gene="ydjM"
                     /locus_tag="ACHOSM_RS07215"
                     /old_locus_tag="pgaptmp_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4557998.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            53694..55085
                     /gene="tcyP"
                     /locus_tag="ACHOSM_RS07220"
                     /old_locus_tag="pgaptmp_001444"
     CDS             53694..55085
                     /gene="tcyP"
                     /locus_tag="ACHOSM_RS07220"
                     /old_locus_tag="pgaptmp_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HGU4557999.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(55089..55893)
                     /locus_tag="ACHOSM_RS07225"
                     /old_locus_tag="pgaptmp_001445"
                     /pseudo
     CDS             complement(55089..55893)
                     /locus_tag="ACHOSM_RS07225"
                     /old_locus_tag="pgaptmp_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        13965 a        14209 c        13453 g        14314 t
ORIGIN      
        1 cgatggaagc atcctgtttt ctctcaattt ttttatctaa aacccagcgt tcgatgcttc
       61 tttgagcgaa cgatcaaaaa taagtgcctt cccatcaaaa aaatattctc aacataaaaa
      121 actttgtgta atacttgtaa cgctacatgg agattaactc aatctagagg gtattaataa
      181 tgaaagctac taaactggta ctgggcgcgg taatcctggg ttctactctg ctggcaggtt
      241 gctccagcaa cgctaaaatc gatcagctgt cttctgacgt tcagactctg aacgctaaag
      301 ttgaccagct gagcaacgac gtgaacgcaa tgcgttccga cgttcaggct gctaaagatg
      361 acgcagctcg tgctaaccag cgtctggaca acatggctac taaataccgc aagtaatagt
      421 acctgtgaag tgaaaaatgg cgcacattgt gcgccatttt ttttgtctgc cgtttaccgc
      481 tactgcgtcg cgcgtaacat attcccttgc tctggttcac cattctgcgc tgactctact
      541 gaaggcgcat tgctggctgc gggagttgct ccactgctca ccgaaaccgg ataccctgcc
      601 cgacgataca acgctttatc gactaacttc tgatctacag ccttattgtc tttaaattgc
      661 gtaaagcctg ctggcagtgt gtatggcatt gtctgaacgt tttgctgttc ttctgctgat
      721 agtggtcgat gtacttcaac ataacgcatc ccgttaggtt ccacggaata tttcaccggt
      781 tcgttgatca ctttcaccgg cgttcccgtc cgcacgctgg agaacaaggc tttaatatcc
      841 ggtgcattca tgcgaataca ccctgaactg acgcgcaaac caacgctgtc cggcgcacta
      901 gtgccatgaa tgaggtattc gccattacca tgcgcgaggc gcagtgcgta acgtcctagc
      961 gggttatttg gtccggcagg aacgactggc ggtaatttaa tgccacgctc cagcgaacgc
     1021 tgacgaatgc ctgccgtagg cgtccaggtt gggttaggga ttttctgccc aacacgcgtt
     1081 tccatcaccg gcgtttccag cccctgcaat ccaataccta ttggataaac ctgcacaata
     1141 ttttctcccg gcggataata ataaaggcgc agctctgcaa ggttaacgat aatcccctga
     1201 cgcggtgcat caggtaataa cagttgtgaa ggaatagtta tcgtcgtacc aggttttggc
     1261 accggggcga tagtgttatt ggcttcaagg atcaacattg ccgcagtatc aaaacgtcgg
     1321 gcaatagcct gaaggttttt atccccttct tgcaccgtac acgtttgatt ttgcccaacc
     1381 agtcggcttc cggttggtgg tagcggataa tcaaccgccc aggcagcctg gatggcgcta
     1441 aaagcgccga taagcgtgag tgtaagcaaa gacgcgcgtt tcattgtaaa cctcctgtat
     1501 ttgccggaga ctcacgctga aacgtcggat ggcgcttatg ttcacctaaa accaaaacac
     1561 tcctgtgcag gtcagtgtaa acattgacca tccggcaatg tgagccaacc ggatgaaagc
     1621 tgtcccttta gtttagctaa gtgcagcggc tttggcgcga attgcgcgaa tcatcgcttc
     1681 cagaccttgt gaacgagatg gggtgagatg ttgggtgagc gccatttttt taaaccacgg
     1741 acgcacatcg aaattgacaa tatcctgcgg cgtcatctga tcgtagagaa taaagacgac
     1801 cgcaataagc cctttcacaa tcgccgcatc gctgtcgccc tgcaattcaa taattccctg
     1861 cgcattctgg cgcatgacaa tccacacctg actctggcag ccctgaatgc tattttgtgg
     1921 gcttcggtct tcggcgcgta attctggcag acgctggccc agctcaataa tgtagagata
     1981 tttctcttcc cagttggcgc agcgtaaaaa attacgcagc aatttttctt tatccggcaa
     2041 taaagccata gtgcctccct gttatcccag cagacggtga atacgttgca gtccggtcac
     2101 cagacgatcc acttcttcat gggtgttata catggccagc gacgcccgac acatagcagg
     2161 gacgttgtaa taggccatta atggcatagc gcagtgatgt ccggtacgca cagcaatgcc
     2221 gtagttatcg agaaaactgc caacatcata ggcgtggtgt ttaccaagat taaaagcaat
     2281 aacgccaagc ctgttccgcg ggccatagag agtgagatcc gatacagatt ccagctgtga
     2341 tagcgcgtag tgcatcagat tctgttcata ctcggctatg ttattaagcc ccagcgccga
     2401 aacatactcc agcgccgcgc caagaccaat gatgcccccg gtattgggtg taccggcttc
     2461 aaaccgccat ggtgctttgg tccaggtagt gccttcactc aggctgacgg tggcgatcat
     2521 agaaccgccc ccttcccacg gcggcatctc ctgcaacaag gcttctttga cataaagaat
     2581 gccaatcccg gtggggccat acagtttatg cccggaaaac acgtaaaaat cgcaatccag
     2641 cgcctgaaca tccaccggat gatgcatcac cgcctgagcg ccatccacca gcacttttgc
     2701 gccatgctgg tgcgcaagcg tgatcattcc cgccagtgga ttttctgtgc caagcacgtt
     2761 ggagacatga gtaattgcca gaaggcgagt tttctcatca aacagcgtag gcagcgtctc
     2821 cagttgcagc gtaccgtcgg ggttgagagg gatcacacgc agctctgcgc caacgcgtgc
     2881 gcaaagcatc tgccagggga caatgttagc gtggtgctcc atctgactga tgatgatgtt
     2941 atcgcccgcc cgcacattgc tgttgcccca gctattggcg accagattga tcccttccgt
     3001 cgtgccgcgg acgaacacca gctcttccgc cgaacgggca ttaataaaca gcgatgcccg
     3061 cttgcgcacg ttctccattt tctcggtcgc ctgggcgctt aaggtatgaa taccgcgatg
     3121 caccgccgcg tagccatgac gataaaactc ggcctcggcg tcaatcacct ggctcggttt
     3181 ctgtgcactg gcggcgctgt cgagataagc cagcggcaaa ccgtttacct cacgcgaaag
     3241 caccggaaag tcggcccgca ctttgtcgac ggaaaaagtc atcttgcacc tcctggcagc
     3301 cgttgaccga ttcgggccag cacctgctgt ttaagcccct catcacgcag tgcttccgtc
     3361 agttcggcag caaaggcgta aatgatcatc tgctgggcat cctgctgatt gatcccgcgc
     3421 gagcgcagat agaacatctg ttcatcatca atacgcccca ccgtcgcgcc gtggctgcat
     3481 ttcacatcat ctgcatagat ttccagctgc ggtttcgtat ccacttccgc cagtttgccc
     3541 atcagcagat tattgttggt catctgacca tccgttttga tggcgtgctg cgcgacgttg
     3601 atcaaaccgt taaataccgc gcggcctttg tcgctgacga tagttttgtg caactgtcgg
     3661 ctgttacaaa aacctttatt gtgttccagc caggtacggg tatcacacac ctcgtttttc
     3721 accggcatcg ccaggctatt gatccgcagc gtgctgtttt cgccattgag ttgcgtactg
     3781 gtgttgtgtc gtaacactgc gccacccagc aggaaactat ggctaaatgc ggtggcatcc
     3841 tcagccagca acaaatcgtt atgagcaaag tggtgactga gcgggttttc aaacgccagc
     3901 ttgatatgct gcaagtgggc gttcgctgcg acattgatag tgaaccgtgc cccggtaaaa
     3961 tgacgagcat cattaaggct gacaaaatgt tcgatcaccg ttgcttcggc accttccgcc
     4021 agatccagat gatgccggta atgggcagtg ttcacctctt cacctgccac gccctgggtg
     4081 atatacatta acagcaatgg ctttgccggc cgttgaccgc gcttcacggc gatatgcgtc
     4141 acgctttgtg ccaggctttc cgtcaaatgc agaaacactt ccgcctgaat agcgtcgggt
     4201 aacccctgac ggtcgtcgtt aatgctcact tcatatccgc tgccttcaat tgcatcgctc
     4261 agtgctgaca cgtaacgtcc atcgacaaac accagccgca cggcgtctaa cgttaacgct
     4321 aaggcatcac gctgctgtgg ggatatctcc cccgcaatgc tgacaaactg gctattggtc
     4381 agcccttcca gcggcgtata tttccagttt tcatgtttac gtgtcggcag tccggtacgc
     4441 agcaattgtt gtaaatgctg ctgtgcttgc ggggagcgtt ttgtcccttc agcttcaaac
     4501 aagtgatgcc actgttgcag cgcgttactg ctgttcggta agccagccat aaccctgctc
     4561 ctccagttgt ttgaccaacg tgaaatcgcc ggatttcaca attcgtccct gatatagcac
     4621 atggacgtaa tcaggcttga tgtagtcgag aatgcgttgg tagtgcgtaa caatgatgaa
     4681 tgagcgcttg ccatcacgca gcgagttcac gccatcggcg accactttta atgcgtcaat
     4741 atccagcccg gagtccgact catcaagaat gcataactcc ggttccagca ccgccatttg
     4801 caaaatatcg ttgcgctttt tctcgccgcc ggaaaaacca acgtttaccg aacgggttaa
     4861 taaatcttcc ggcatcttca ggagagcgat tttctcttcc atcaaatcct gaaaatcaaa
     4921 gcggtcgagc gtttcctggc cgcgatagct gcgcactgca ttaagtgccg tttgcaggaa
     4981 aaactggtta ctgacgcctg gaatctccac cggatactgg aaggccataa agatgccttc
     5041 gcctgcacga tcttccggcg acagtgcaag caaatctttg cctttgaact caaccgtgcc
     5101 gcccgtcact tcataatctt cgcgcccggc aagcgttgcc gataaggtac ttttgcccga
     5161 accgtttggc cccataatgg cgtgaacttc gccgggacga acggcgaggc ttaatccacg
     5221 caggatagct ttatcttcca cgctgacgtg taaatcttta atacttaaca tgtttattcc
     5281 ttatccgacg ctgtgttcaa gactgatggc gaggagtttt tgtgcttcaa cggcaaattc
     5341 cagcggcagt tctgagaaca cgtctttgca gaaaccgtta acaatcatcg agatggcgtc
     5401 ttcttcgctg atcccgcgtt gcaggcagta aaacagttga tcttcaccaa tacgtgatgt
     5461 cgttgcctcg tgctccagtt gtgcgctgtt attgcgacat tcgacatacg ggaaggtatg
     5521 cgccccacaa ttagcgccaa tcagcattga gtcgcactga gtgaaattgc gcgcattggt
     5581 tgccgtcggc atgattttca ctaagccgcg ataactgttc tgactatgtc cggcagagat
     5641 ccctttcgag ataatggtcg atttggtgtt tttaccgatg tggatcatct tggtgccggt
     5701 atccgcttgc tgatgaccgc tggtcagcgc cactgagtaa aactcaccaa tggagttatc
     5761 gccgcgcaaa atgcagctgg gatatttcca cgtaatcgcg gacccggttt ctgattgcgt
     5821 ccatgacatt ttgctgtttt cgccttcgca caaagcacgc ttggtgacga agttgagaat
     5881 accgccggtg ttgttatcgc cagggaacca gttttgtacc gtggaatatt tcacctcggc
     5941 gtttttatgg atgatgactt ccaccactgc cgcgtgtaac tgatagctgt cacgcaccgg
     6001 agcggaacag ccttcaatgt agctgacgta gctgtcttcg tcggccacca gaatggtgcg
     6061 ctcaaactgc ccggtttttt ccgcgttaat gcgaaaatag gtggaaagtt ccatcgggca
     6121 gcgcacgcct ttaggcacat aaataaacgt accgtcagag gctaccgccg cattaagcgc
     6181 ggcaaagaag ttgtcattcc ccggcaccac ggtgccgaga tatttacgca ccagttccgg
     6241 gtgatcgtgg atcgcctcac caaaggaaca gaaaataatt ccctgctccg ccagtttttc
     6301 gcgataagta gtggcaaccg aaacagagtc gaaaatggca tccaccgcca cctctttgcc
     6361 ttcccgcacg ggaacgccca actgctcaaa cgccgcctcc acctctttac ttaaaaaggc
     6421 gttcgcgcca gtttgctgca ccgcgccagg ttcagacgcg caagtgtcgt cacaattacc
     6481 gcacgatggt gctgagtagt agctgtaatc ctgataattc agcttgtcgt agtgcgcttt
     6541 caaccagtgc ggttcttcca tctccagcca tgcgcgatag gcctttagac gaaactccag
     6601 catccactcc ggctcattac gcttcgccga aattgcgcgc accacctctt cgtttatccc
     6661 ctttgccagc tcatcggtgg ctaactgggt gaagaatcct tctttataat tcagcgggcc
     6721 gccggtccag gttttgacat cgtcagttgc ttcagtatta cgagacatag taccgcctat
     6781 accccaaagc tttcgccaca gccacattca ttctgggctt tagggttgtg aaatttgaat
     6841 atctgattaa gtccttcacg aacgaaatcg acttccgtgc catcaataaa cggcatcgct
     6901 tgcagcggga caaacagctt cgcgccgtcg tgttcaaaca gcagatcgtc tttgtccggc
     6961 tcgctaacac tgtcgagcac atagccaaag cccgcgcagc ccgtttgctt cacgcctaag
     7021 cgcacgccga ccatacccgg ctgctttgcc accagctcac ggatgtgtat cgccgctgcg
     7081 ggtgtcagcg ttaagccttg ccaggcgaaa tcttgtgggt taaaggttcc tgaatgcatg
     7141 tccatcgatt tacctcactt catcgctttc agcgtataac agcatgttag tgataatgat
     7201 tatcagttca acccagcaaa cgcaggggct ttaccgtaaa acatgctttt tgcctgcttt
     7261 taataagcat agaccctgat gtgtgggtta acaggcacgc taagaattag gtatctcatt
     7321 gttagataat ggttattatc taaggagcat taaggctgtg atgaaaaaga aaaagttgta
     7381 ttgaaaatga ctatttaaga gataggtaaa aaagtgcagc gttcagaaat aagaaaaccc
     7441 ttaagtctgt gcgacacagg cttaagggtt tctaccccat ccggcgctta tctccggcac
     7501 tctcaggggc ttagctcttg aaggggcgat aagaataatc tcataaagct aacccgccgt
     7561 tttaacacaa actgcgatta gtattatttt tgaacaatat caggcggtag ataagcagta
     7621 ttaagaaggt catcgaacct ggacggaggt taatccaggt cgatttggcg aacttgcggc
     7681 attaagtcag gatcaatgca cgcccagacg ccaggcaaag tagatttctt cttttaattc
     7741 agcagaagag agagtaagca ggtcagcaaa ttcaagttct agttgtttca gacgtttgag
     7801 atattgggca ggtgaaagat tgctctggtc acggcgtaaa aattcaatgg ccagctgggt
     7861 gggatcaagt tgagtagaca tagcatcctc gcttttagac aagacgtgca cagtatacca
     7921 ccgtttactg tgcagataat gaccaaaagc aatatgcgtc acacttttct ggtgacaacg
     7981 tcacaaaatg gcggtcgtca atcgtgacga acagcacaaa cgccctttct catcgaagat
     8041 ttcaatctgc cagacctggt gacgcgaacc gagatgcaac ggtttgcata cgccgcgcac
     8101 ccgcccttct cgtgccgagc ggacgtggtt agcattgatt tccagaccaa ccactttttg
     8161 ctcaccttcg gtacataaat aaccggcaac ggaaccgata ctttcggcca gtaccacgga
     8221 tgcacctcca tgtagcaacc cgaaaggctg ctttgtccgc gagtctactg gcattgtcgc
     8281 ttcaagggtg tcatcaccaa tatgttcaaa gcgaatatcc agtaacccca ccatgtttcc
     8341 ttcacccatg gcattcagtg cttccagggt gatttttcgt ttccatatca tttagtaatc
     8401 tccagtaaag cctgcacagg atggcgtacc cccgtgcctt caacccgttt gacctgacta
     8461 cggcaggaat atccggtcgc cagacagcgg ttacgcggca gtcgctgcat agcctgatgc
     8521 caggataact catagatccc gagcgaattt ttatggtttt tcgcttcatg tccgtaagtc
     8581 cctgccatgc cgcagcaacc cacgctgaca ttttccagtt tcgcgccaaa acgggcaaat
     8641 atcgcggccc attgtgctgg cgcacccggc aaggcggtaa cttcggtaca gtgaccaaag
     8701 aaataccatg attcaccgct gactgtagcc actggctgtg actcaagtgc gcttgccagc
     8761 cattcattcg ccagtaagac gttaaactcg ccacgctcct cgcccagcgc cagtttatat
     8821 tcatcgcgat aacaaagtac cagcgccgga tcgacgccca ccattggcat acccagcttc
     8881 gccatacggt tgaggaaatc cgccgtcttt ttcgccgtct tcgcaaaacg attaagaaaa
     8941 cctttaatat gctgggcttt gccatttggc gaaaatggca gtaacacagg ctggaaacct
     9001 aatttttcga ccagacggac aaaatccgcc accacttgcg catcgtaata gctggtaaag
     9061 gggtcctgca ccaccaacac tgtgcgcgct ttctgctctg cattgagaga ttcaagctgt
     9121 tccagcgtca tgtttgccga gcgatgcccc accatttgtt gttgtagcga ggggaccgac
     9181 agcagcggca aatcaaccat gccgatatgt ttttccgaga gtttgcgcac cagcggctgg
     9241 ttaatgaaga agttaaaggt tttcggcgcg cgtgccatca gcggcgcgta gctctcgacc
     9301 gtagcgacga ggtggtcgcg cagcgggcgt aaataacggg tgtgatagag ctgcagaaaa
     9361 cgagagcgaa actccggcac atcaattttg atggggcact gggtcgaaca cgctttacag
     9421 gccagacagc ccgacatcgc ctctttgact tcgtgtgaga agtcatattc gcctttattc
     9481 gcatgccagc tattgcgcgt gcgggcaatt aacgtccgca aactgacgcc ggattcaggc
     9541 agttcttgtt ccagtttgag tggatcaacg ccgcgatccg ccaacaaacg cagccattca
     9601 cgcaccagcg ttgcgcgccc tttcggtgaa tgaatccggt tctgggtgat cttcatcgac
     9661 ggacacatcg gactacgggc atcaaagttg aagcataaac cgttgccgtt acactccatc
     9721 gcaccgcgcc actgctggcg taccgcaatg gggatctgcc gatcgaatgt accgcgcttc
     9781 accgcgtcca ctttcatcat cggcgcatcg agaccttctg gcgggcaaat cttccctggg
     9841 ttgagtcggt tatgcgggtc aaatgccgct ttcactttgc gcagttctgc aaaaagttcc
     9901 tcaccgaaaa acgccgggct gtattcagcg cgaaaacctt tgccgtgctc gccccacaac
     9961 aaaccaccgt atttcgcagt cagcgccacc acgtcatcag agatttgctt catcaaaatc
    10021 tcttgttgag gatcgcacat atccagcgct ggacggacgt gcaagacacc tgcgtcgacg
    10081 tgaccgaaca taccgtagct taagccgtgg ctgtcgagca gcgcgcgaaa ttcagcaata
    10141 taatccgcca ggtgttccgg cggtacgcag gtatcctcag caaacggaat tggcttagcg
    10201 gcacctttgg cattgccaag cagaccaacg gcttttttgc gcattgcata gatacgttca
    10261 acccccgcca gctcacggca cacctgccag ccgatgacac ctgcttgatg actggcgatc
    10321 agctcatcca accgcgcaca gagtgcattt acccgctcat caatcagcgc ctcatcatca
    10381 ccagcaaatt ccacaatgtt cagcccgagc atctcttggt caggcacatc ggtaatcaac
    10441 tcgctgacgg aatgccagac aatatcttcc cgcgccagat tcagcacttt tgagtccacc
    10501 gtctctaccg aaagcgcccg cgcctcaacc ataaacggcg cgttacgcag cgcagagtca
    10561 aaagagtcat atttgacgtt caccagacgg cgcactttag gcaagcgcgt aatatccagc
    10621 cgcgcttcgg taataaaggc cagcgtccct tctgaacccg tcagaatgcg cgtcaggtcg
    10681 aactcggtca tctcatcgtt aaagacatga cgcagatcgt aaccggtaag aaagcggtta
    10741 agtttgggga agttgtcgat aattaactgg cgttgctgac ggcaacgttg ataaaccgtg
    10801 ttataaattc gcccgattgt ggtattggat ttacccagcg tttccgccaa ttcgacgggt
    10861 aaaggttgcg tatcgagaat atcgcccccc aacaacaccg cgcgtacgcc aagtacgtga
    10921 tctgacgttt tgccatagac cagcgatccc tgaccggatg catcggtatt gatcatccca
    10981 ccgagcgttg cccggttgct ggtcgaaagt tccggcgcaa aaaagtagcc gaacggtttc
    11041 aggtactgat tgagttgatc ttttatcacc ccggcctcaa cgcgcaccca gccctcttca
    11101 gggttaattt cgatgatgcg gttcatatgg cgggacatat caacaataat cccctggttg
    11161 agcgcctgac cgttagtgcc ggtgccgccg ccgcgggggg taaagatcag cgatgaatag
    11221 cgttcctgcg cggcaagacg ggcgatcagc gccacatctg cggttgaacg cggaaatacc
    11281 accgcatcgg ggagaagttg gtaaatactg ttgtcggtcg acattgtcag acgatcggca
    11341 taacttgtcg ccgtatcgcc ggtaaaacct tgttgctcca gctcttgcaa aaaattaagc
    11401 accagttgaa cgacgccggg tgcctgggaa atctgtggaa tcattatatt gaccctttcc
    11461 tgcggtctgt gatgtaggtc gatacactgt tctttcaggc ggctgcaata gcgcacagaa
    11521 aggtgatgtt tatttactct atggatttcg agttgcagga aggcggcaag cgagtgaact
    11581 ccaggagctt acaacagtaa gtaactgggg tgaacgaacg tagccgcagc atatgcaact
    11641 tgaaatacga cgagtaaatc gtttgcgtgt tgcctgagtt gttgtaccac atttttttct
    11701 aacacgccca tcagaattaa gggcagaatc ggcctgttaa aaaccgctga aattgctcat
    11761 cattatgtag gtgagtttcg cgtgttcacg tcgcgtcgac gatttgacgc acaaaaaagg
    11821 tgaaaagtag ttatggtaaa tgttcgtcag cccagggatg tcgcacaaat tctgctttcg
    11881 gtgctgtttt tagccatcat gattgtggca tgtctgtgga ttgttcaacc ctttattctc
    11941 ggctttgcat gggccggtac ggtggttatc gccacctggc cggtattgtt acgtttgcaa
    12001 aagatcatgt ttggccgccg ctctctcgcc gttctggtga tgacgctgtt attagtgatg
    12061 gtgtttatca tccctattgc tttgctggtt aacagtatcg tcgacggcag cggcccgcta
    12121 attaaagcca tttccagcgg cgacatgacg ttacccgatc tggcgtggct taataccatt
    12181 ccggtgattg gcgcgaagct gtatgcaggc tggcacaact tgctggatat gggggggacg
    12241 gcgatcatgg cgaaagtccg cccttatatt ggcaccacca ccacctggtt cgttgggcag
    12301 gcggcgcata tcgggcgctt tatggtgcat tgtgcgctga tgcttctctt cagtgccctg
    12361 ctgtactggc gcggtgaaca ggtggcacaa ggcattcgcc attttgcaac ccgtctggca
    12421 ggcgttcgcg gagatgccgc cgtgctgctg gcggcacagg ctatccgcgc ggtggcgctg
    12481 ggtgtggtgg tgacggcgtt agtacaggca gtgcttggcg gtatcggcct cgccgtatcc
    12541 ggcgtacctt atgcaacgtt gctaacggtg ttaatgatcc tctcctgcct tgtccagctt
    12601 ggcccgttgc cggtactgat tccggcgatt atctggctct actggactgg cgataccacc
    12661 tggggaacgg tattgttagt gtggagcggt gtggttggca cgctggataa cgtcatccgc
    12721 ccaatgttaa ttcgcatggg tgccgattta ccgctgatcc tgatcctctc tggtgttatt
    12781 ggtggtttga ttgctttcgg gatgatcggt ctgtttattg gtccggttct gttagccgtt
    12841 tcctggcgtc tgtttgccgc gtgggtggaa gaagtcccgc cgccgacgga ccaaccggaa
    12901 gaaattctcg aagaacttgg cgaaatcgag aaaccgaata agtaatttct catcaggcgg
    12961 ctctgccgcc tgattgttaa cccccgctaa ttgatatttg cttgctcttc cccatatttt
    13021 cctgcttacc gccattcagc tggtagtacc tgtcgcaaat tctttacagt ttttaaacta
    13081 atgagacgaa tctgatcgac gcaaaaagtc cgtatgccta ctattagctt acggttataa
    13141 atcaacatat tgatttataa gcatggaaat cccctgagtg aaacaacgaa ttgctgtgtg
    13201 tagtctttgc ccatctccca cgatgggctt ttttttaaca tttttccgct tcgctacctc
    13261 gcccctcact ctttccaata atgttgcaca agatactgtg actctcttca agccgtcagc
    13321 aggctgataa tgttatttat attttattgt ttatttcatg cagccattac acgatgaacg
    13381 cttatgaact ccaggcactt cgccatattt ttgccatgac tattgatgaa tgcgcgacct
    13441 ggattgccca gaccggtgac agtgaaagct ggcgtcagtg ggaaaatggc aaatgcgcca
    13501 ttcctgatcg tgtagtcgag caactgttgg ctatgcgtca gcaacgaaaa aaacatcttc
    13561 atgccatcat agagaaaata aataaccgca tcggcaacaa caccatgcgt tttttccccg
    13621 acttaaccgc gtttcaacaa gtctaccctg atggcaattt tattgactgg aagatctatc
    13681 aatcggtagc cgctgaatta tatgcacacg atctggagcg gctttgttaa tttctccaca
    13741 aaaaggaatt gtcgttgtta caacaataat gaacggatac tgacacaaca tcgctacact
    13801 ttttaaagca cctttgctaa gtagaaccta tgaaaaatcc ctattaccct accgcacttg
    13861 ggttgtattt taattacctg gtgcatggta tgggcgtcat tttgatgagc ctgaatatgg
    13921 cctcgctgga gacgctttgg cagactaatg ccgcgggtgt ctcgatagtt atctcatcgc
    13981 tgggcattgg tcgattaagt gtcttgcttt ttgcaggatt attatccgat cgctttggtc
    14041 gccgcccttt tatcatgctc gggatgtgct gctatatggc cttctttttt ggcatcctgc
    14101 acaccaataa catcattatc gcttatgttt ttggctttct ggcaggaatg gcaaacagtt
    14161 ttctcgatgc aggcacttat cccagtttga tggaagcttt tccacgctca cctgggacag
    14221 ccaatatttt aattaaagca tttgtttcca gcggacaatt tttattaccg ctaatcatta
    14281 gcctgttagt gtgggctgaa ctgtggttcg gttggtcctt tatgattgct gcaggcatta
    14341 tgtttattaa cgctctgttt ttataccgtt gtacgttccc accccatccg ggtcatcgct
    14401 tacctgtcat aaagaaaacc accagctcta cggaacatcg ctgttcaatt atcgatttag
    14461 ccagttatac cttatatggc tatatctcaa tggcaacgtt ttatctggtt agccagtggc
    14521 tggcacagta cggacaattt gttgcaggca tgtcatacac tatgtcgatt aaactactca
    14581 gtatctacac cgtgggttcg ctgctttgtg tatttattac cgctccactc attcgtaata
    14641 ccgttcgccc aacaacatta ctgatgctgt acacctttat ctcatttatt gctctgttta
    14701 ccgtctgcct gcatcccaca ttttatgtgg tgataatatt tgcttttgtc attggtttta
    14761 cctctgctgg aggtgttgtg caaattggcc tgacgttaat ggctgaacgt ttcccttacg
    14821 ctaaaggtaa agctacaggg atctattaca gtgcgggcag tattgcgacc tttactattc
    14881 cgttgattac ggctcatctg tcccaaagaa gtattgccga tattatgtgg ttcgataccg
    14941 ccatcgctgc catcggtttt ttactggcac tgtttatcgg cttacgcagc cgcaaaaaaa
    15001 cgcggcatca ctcgctaaag gaaaatgtcg ctccgggtgg gtaatgcaat attcttttca
    15061 ggtcatgcaa gatcttacgg ataaataact ctttctaact aaggaaaatc gcgatcaaaa
    15121 acaaactatg acatgcaata ttcctggaga cataaacttt atgccatgta cccagggaaa
    15181 atcatcttca gtatagtaat tatgtaaacc gtcggagaac aatacgtacg gtaacgaaat
    15241 tatctttcag caaggagctg tgaaaatgtc tcaaaataag gctttcagca cgccatttat
    15301 cctggctgtt ctttgtattt acttcagcta cttcctgcac ggcattagtg ttattacgct
    15361 tgcccaaaac atgtcatctc tggcggaaaa gttttccact gacaacgcgg gcattgccta
    15421 tttaatttcc ggtatcggtt tggggcgatt gatcagtatt ttattcttcg gtgtgatctc
    15481 cgataagttt ggtcgtcggg cggtgatatt aatggcagta ataatgtatc tgctattctt
    15541 ctttggtatt cccgcttgcc cgaatttaac tctcgcctac ggtctggcag tgtgcgtagg
    15601 tatcgctaac tcagcgctgg atacgggtgg ctaccccgcg ctcatggaat gctttccgaa
    15661 agcctctggt tcggcggtca tactggttaa agcgatggtg tcatttgggc aaatgttcta
    15721 cccaatgctg gtgagctata tgttgctcaa taatatctgg tacggctatg ggctgattat
    15781 tccgggtatt ctgtttgtac tgatcacgct gatgctgttg aaaagcaaat tccccagcca
    15841 gttggtggac gccagcgtag ctaatgaatt accgcaaatg aacagcaaac cgttagtctg
    15901 gctggaaggt gtttcatcgg tactgttcgg tgtagccgca ttctcgacct tttatgtgat
    15961 tgtggtgtgg atgcccaaat atgcgatggc ttttgctggt atgtcagaag ctgaggcatt
    16021 aaaaaccatc tcttattaca gtatgggctc gttggtctgt gtctttattt ttgccgcact
    16081 actgaaaaaa atggtccggc ccatctgggc taatgtattt aactctgcac tggcaacaat
    16141 aacagcagcc attatctatc tgtacccttc tccactggtg tgtaatgccg gcgcctttgt
    16201 tatcggtttc tcagcagctg gcggcatttt acagctcggt gtttcggtca tgtcagagtt
    16261 tttcccaaaa agcaaagcca aagtcaccag tatttatatg atgatgggtg gactggctaa
    16321 ctttgttatt ccgctgatta ccggttatct gtcgaacatc ggccttcaat atatcattgt
    16381 tctcgatttt actttcgcgc tgctggccct gattaccgca attattgttt ttatccgcta
    16441 ttaccgcgtt tttattattc ctgagaatga tgtgcggttt ggcgagcgta aatttagcac
    16501 ccggttatac acaattaagc atagaggtta aaggagttaa ttatggatgt taccgcaaaa
    16561 tacgaattga ttgggctgat ggcctatcct atccgccaca gtttatcgcc tgaaatgcag
    16621 aataaagcct tagaaaaagc gggattgcca tttacctata tggcctttga agtggataac
    16681 gatagttttc ccggagcaat tgaaggatta aaagccctca aaatgcgcgg aactggtgtg
    16741 tcgatgccga acaaacaact ggcgtgtgaa tatgttgatg aattaacgcc agcagccaaa
    16801 ctggtgggtg ccatcaacac catcgttaat gatgatggct acctgcgcgg ctataacacc
    16861 gacggcacgg gccatattcg cgccattaaa gagagcggtt ttgatatcaa aggcaaaacg
    16921 atggtgctgt taggggccgg aggtgcctca acggcgattg gcgcgcaggg ggcaattgaa
    16981 ggtttaaaag aaattaaact ctttaaccgt cgggatgagt tcttcgataa agccctcgcc
    17041 ttcgcgcagc gggttaacga aaacaccgat tgtgtcgtca cggttaccga tctcgccgat
    17101 cagcaagcct ttgctgaagc cctggcttcc gccgacattt taaccaatgg cacaaaagtg
    17161 ggtatgaaac cccttgagaa tgaatcattg gtttatgata tcagtctgtt acatccggga
    17221 ctgctggtca ctgaatgcgt gtataacccg catatgacga agttattgca gcaggcacaa
    17281 caagctggtt gcaaaacgat tgatggatac ggcatgttgt tgtggcaagg ggctgaacag
    17341 ttcacgttat ggactggcaa agatttccct ctggaatatg ttaaacaggt catggggttc
    17401 ggtgcctgac aggctgaccg cgtgcagaaa gggtaaaaaa tgaaaaccgt aactgtaaaa
    17461 gatctcgtca ttggtgcggg cgcacctaaa atcatcgtct cgctgatggc gaaagatatc
    17521 gcccgcgtga aatccgaagc tctcgcctat cgtgaagcgg actttgatat tctggaatgg
    17581 cgtgtggacc actttgccga cctctccaat gtggagtctg tcatggcggc ggcaaaaatt
    17641 ctccgtgaaa ccatgccaga aaaaccgctg ctgtttacct tccgcagtgc caaagaaggc
    17701 ggcgagcagg cgatttccac cgaggcttat attgctctca atcgtgcagc catcgacagc
    17761 ggcctggttg atatgatcga tctggagtta tttaccggcg atgatcaggt taaagaaacc
    17821 gtcgcctgcg cccacgcgca tgatgtgaaa gttgtcatgt ccaaccatga cttccataaa
    17881 acgccggaag ccgaagaaat cattgcccgt ctgcgcaaaa tgcagtcctt cgacgccgat
    17941 attcctaaga ttgcgctgat gccgcaaagt accagcgatg tgctgacgtt gcttgccgcg
    18001 accctggaga tgcaggagca gtatgccgat cgtccaatca tcacaatgtc aatggcaaaa
    18061 actggcgtaa tttctcgtct ggctggtgaa gtatttggct cggcggcaac ttttggtgcg
    18121 gtaaaaaaag cctctgcgcc agggcaaatc tcggtaaatg atttacgcac agtattaact
    18181 attttacatc aggcataagc aataatattt cggcgggaat atcctcccct cccccgccga
    18241 actaaaaaaa atatattcaa ccgtatttaa taaaaatatt tcgtgagtct ctgtgcgcta
    18301 attatccatc tggcgtaggg aaaatcacac ctgaatcagg aattaacaat gaaacctgta
    18361 aaaccacctc gtattaatgg acgagtgccg gtcctgtcgg cacaggaagc ggtgaattat
    18421 attcccgacg aagcaacact ttgtgtgtta ggcgctggcg gcggtattct ggaagccacg
    18481 acgttaatta ctgctcttgc tgataaatat aaacagactc aaacgccacg taatttatcg
    18541 attattagtc caacagggct tggcgatcgc gccgaccgtg gtattagtcc gctggcgcaa
    18601 gaaggtctgg tgaaatgggc attatgtggt cactggggac aatcgccgcg tatttctgaa
    18661 ctcgcagaac aaaataaaat tattgcttat aactatccac aaggtgtact tacacaaacc
    18721 ttacgcgccg ccgcagccca ccagcctggt attattagtg atattggcat cgggacattt
    18781 gtcgatccac gccagcaagg cggcaaactg aatgaagtca cgaaagaaga cctgattaaa
    18841 ctggtcgagt ttgataacaa agaatatctc tattacaaag cgattgcgcc agatatcgcc
    18901 ttcattcgcg ctaccacctg cgacagtgaa ggctacgcca cttttgaaga tgaggtgatg
    18961 tatctcgacg cattggttat tgcccaggcg gtgtacaata acggcggtat tgtgatgatg
    19021 caggtgcaga aaatggttaa gaaagccacg ctgcatccta aatctgtccg tattccgggt
    19081 tatctggtgg atattgtggt ggtcgatccg gatcaaagcc aactgtatgg cggtgcgccg
    19141 gttaaccgct ttatttctgg tgacttcacc cttgatgaca gcaccaaact tagcctgccc
    19201 ctaaaccaac gtaaattagt tgcgaggcgt gcattattcg aaatgcgtaa aggcgcggag
    19261 gggaatgtcg gcgtcggtat tgctgacggt attggcctgg tcgcccggga agaaggttgt
    19321 gctgatgact ttattctgac ggtagaaaca gctccgattg gcggaattac ttcacagggg
    19381 atcgcctttg gcgcgaacgt gaatacccgt gccattctgg atatgacgtc ccagtttgat
    19441 ttttatcacg gtggtggtct ggatgtttgt tatttgagtt ttgctgaagt cgaccagcac
    19501 ggtaacgtcg gcgtgcataa attcaatggc aaaatcatgg gtaccggtgg atttattgat
    19561 atcagtgcca cctcgaagaa aattattttc tgcggcacgt taactgcggg cagtttaaaa
    19621 acagaaattg ccgatggcaa attgcatatc gtccaggaag gacgggtaaa taaatttatt
    19681 cgggaactac cggaaattac ttttagcgga aaaatcgcgc tcgaacgggg actggatgtt
    19741 cgttacatca ccgaacgtgc tgtattcacg ctgaaagaag acggcctgca tttaatcgaa
    19801 atcgcccctg gcgtcgattt acaaaaagat attctcgaca aaatggactt caccccagtg
    19861 atttcgccag aactcaaact gatggacgaa agattattta tcgatgcggc gatgggtttt
    19921 gtcctgcctg aagcggctca ttaacaggag tataatgatg gatttttctt taactgaaga
    19981 acaagaactg ctgctggcca gtattcgcga actgattacg actaactttc cggaagagta
    20041 tttccgcacc tgcgatcaaa acgggacata tccgcgtgag tttatgcggg cgctggcgga
    20101 taacggtatt tccatgcttg gcgtgccgga agaatttggt ggtatccctg cggattacgt
    20161 cacccaaatg ctggcgctga tggaagtgtc aaaatgcggt gctccggcat ttttaattac
    20221 caacggtcaa tgtattcaca gtatgcgccg tttcggttct gcagagcagc tacgtaaaac
    20281 ggcagagagc accctggaaa cgggtgatcc cgcctatgcc ctggcgttga cggaaccagg
    20341 tgctggctca gataacaaca gtgccactac cacttacacg cgtaaaaacg gcaaggtcta
    20401 catcaacgga caaaaaacct ttattaccgg tgcgaaagag tacccgtata tgctggtgct
    20461 ggcgcgcgat ccgcaaccga aagatcccaa aaaagccttc accctgtggt gggtcgactc
    20521 cagtaagccc ggcattaaga ttaacccgct gcataaaatc ggctggcata tgctcagcac
    20581 ctgcgaagtc tatctcgaca atgtggaagt tgaagagagc gacatggtgg gcgaagaagg
    20641 aatgggtttc ctcaatgtga tgtacaactt tgagatggag cgcctgatca acgccgcgcg
    20701 cagcaccggc tttgccgaat gcgcctttga agatgccgcc cgctatgcca accaacgtat
    20761 cgcttttggt aagcccattg gtcataacca gatgatccag gaaaaactgg cgctgatggc
    20821 gattaagatc gacaacatgc gcaacatggt gctgaaagtg gcatggcaag ccgatcagca
    20881 tcagtcactg cgcaccagcg cggcgctggc aaaactgtat tgcgcacgta ccgcaatgga
    20941 agtcattgat gatgcgattc aaatcatggg cggtctgggc tataccgatg aagcgcgcgt
    21001 ctcccgcttc tggcgtgatg tccgttgtga acgtatcggc ggcggtacag acgaaattat
    21061 gatttacgta gcaggtcggc agatcctgaa agactatcag aacaaataat ctgcaggcgg
    21121 cgcagcttct taacaaactg cgccgccaga tttatccaac aagacttacc ggttgaggaa
    21181 attcagatag gatttcttgt tccgcaaaac agtttttaaa ttgtctacgg taatcgctgg
    21241 gcgaacaacc gacatggcgc aaaaacagtt tggcaaagtg atcgacattt tcataaccca
    21301 cacgccagga gatctctgcc tgtgataatt cagtattagt gagtgaccat tttgcttccg
    21361 tcatacgccg ttgaataaca tagttaatag gggaaatacg atactctttg gtaaattcat
    21421 ggcaaatata actgacgctg gcacgaaatt ttttcgataa ctgttctaaa gtgatttttt
    21481 cgcgataatt attattcaga tagaaaagaa tatcttttat cagaacatct tttttaatat
    21541 accccagctc cgaacgataa gcatttttaa aattttcgta gtaaagaatg gctaacgtat
    21601 aggcaaatgc atcccataac gaagatgttt ggctattttt actttgcggc aaaatcacac
    21661 ttagctcatt aaataaggtt tttaagactt cttttccctg ccccgcggca attaccggac
    21721 aagaatgcgg ttgcagtagc tgattttcct cagccccctg aaactgaaag ccgtacagcg
    21781 cacaggtaca cgtcgttgcc ggatcgttaa cgtcagaggc caccgcatgc agcctgccgc
    21841 gttctatcac cacaatgtca tccgcatgtg cgacatataa cgaagaatcg atggtaaacc
    21901 ttgcaacccc tttcttgacg taaatcaact cggtttcatt gtcatgaacg tgatgtccgg
    21961 actcccattt tggatcatcg ctaaatgcaa aacgtgaaag ccgtggcgtt ttaccggcga
    22021 caaacaacgt ttcactggcg ttatcaaaac agcgttgata catgacaacc tccctattcc
    22081 atgagcaagc aaaaacaata tatgccggat aaaatacggc gtgtattgcg gttatacaac
    22141 cgcgtttagc gagagctaaa caagattatt tacactgaat gcaagattgt acggtcatgg
    22201 aataactata ttgccgcaaa ttgcactttt gttttagcga tcgcattttt tttgcaagat
    22261 tgttggcaag gaaaacagct tgctccgtcg aaaaccccgc accgctatcg cacactattt
    22321 tcaggccatt tttaccttcc atcggagatg gttccgtatg cgactcacag gagaaatcat
    22381 gaaaataata acctgcttta agctggtgcc tgaagaacag gacattgttg tcactccaga
    22441 atacaccctg aatttcgaca atgccgacgc caaaatcagc cagttcgatc tcaatgccat
    22501 tgaagctgca agccagctcg caacagatga cgatgagata gccgcgctga ccgttggcgg
    22561 ctcattgttg cagaactcga aagtgcgcaa agacgtgcta tcccgcgggc cgcacagcct
    22621 gtatttggtg caggatgcgc aacttgaaca tgcactgcct ctcgataccg caaaagcgct
    22681 ggcggcagca attgaaaaga tcggcttcga tttactgatc tttggtgaag gttccggcga
    22741 cctttatgcc cagcaggttg gcttgctggt cggagaaatt ctgcaacttc cggtgattaa
    22801 tgcagtgagt gctattcagc gtcagggcaa tacactggtg attgaacgca cgcttgaaga
    22861 tgatgttgaa gttattgaac tctctgttcc agccgtgctc tgcgtcacct ccgatattaa
    22921 cgtgccacgc attccttcga tgaaagccat tctcggcgcg ggtaaaaaac cggtaaatca
    22981 gtggcaggca agtgatattg actggagcca gagcgcgcca cttgctgaac tggtcggcat
    23041 tcgcgtaccg ccgcaaacag aacgtaagca catcattatc gataacgatt cgccggaggc
    23101 cattgccgag ctggcggaac atctgaagaa agccctgaac tgaagcctac ggagaagaaa
    23161 cgatgagtca attaaacagc gtctgggtct ttagcgataa tcctgaacgt tatgctgaac
    23221 tgtttggcgg cgctcagcaa tggggccaac aggtgtatgc cattgtacaa aataccgacc
    23281 aggcgcaggc agttatgcct tatggtccaa aatgtattta tgttcttgag caaaacgacg
    23341 cgctgcaacg cactgaaaat tacgccgaaa gcattgctgc cctgctgaaa gataaacacc
    23401 ccgctatgtt gctgttggcc gcgacgaaac gtggtaaagc tctggcagca cggttaagtg
    23461 tgcaactgaa tgcggcgctg gtgaacgatg ccacggcggt ggatattgtc gatggtcaca
    23521 tttgcgccga acaccggatg tatggcgggt tagcgttcgc tcaggaaaag atcaacagcc
    23581 cgctggcgat cattaccctt gcacccggtg ttcaggaacc gtgcaccagt gatacctctc
    23641 atcagtgccc gacagaaacg gtaccttatg ttgctccgcg tcatgaaatt ctctgtcgcg
    23701 aacgccgtgc gaaagccgca agcagcgtgg acctgagcaa agcaaaacgt gtggttggcg
    23761 tcggtcgtgg tctggcggcg caggatgacc tgaaaatggt ccacgaactg gcggcggtgc
    23821 tgaatgctga agtcggctgt tcacgtccaa ttgccgaagg cgagaactgg atggagcgtg
    23881 aacgttatat cggtgtctcc ggcgtgttgc tgaaatccga tctctacctg acgctgggga
    23941 tctccgggca gatccagcat atggttggcg gcaacggcgc aaaagtgatt gtcgccatca
    24001 ataaagataa aaatgcgcca atcttcaatt atgccgacta cggtctggtg ggcgatatct
    24061 acaaagtcgt ccctgccctg attagccagt tgagccgcta attccccttt caaccacatc
    24121 tcccgctgta aacgcagcgg gaagggagca taacgcatgt cggatgacaa atttgatgcc
    24181 attgtggtcg gtgcgggcgt tgctggtagc gttgccgcac tggtcatggc gcgagccggg
    24241 ctggatgtcc tggtgataga acgcggcgac agtgccggat gtaaaaacat gaccggcggg
    24301 cgtctttatg cccacacact tgaagcaatc attccaggct ttgcagtatc agcgccggta
    24361 gaacgcaagg tcacacgcga gaaaatctcc ttcttaaccg aagaaagcgc cgttaccctc
    24421 gattttcacc gcgagcaacc agatgttccg caacacgcat cttataccgt attgcgtaat
    24481 cgtctggacc cgtggttgat ggaacaagcc gagcaggcgg gcgcacagtt tatcccgggc
    24541 gttcgcgtcg atgcgttggt tcgtgaagga aacaaggtca ctggcgtgca ggccggggat
    24601 gatattctcc aagcgaatgt ggtggttcta gctgatggcg taaactcgat gcttggccgc
    24661 tcgctgggaa tggttcccgc ttccgatccg catcattacg ctgttggtgt taaagaggtt
    24721 attggcctca caccagaaca gatcaacgat cgctttaata ttacgggcga ggaaggtgcc
    24781 gcctggctgt ttgccggttc cccttctgac ggcctgatgg gcgggggatt tctctatacc
    24841 aacaaggatt ccatatcctt ggggctggtt tgtggattgg gtgatatcgc ccatgcgcaa
    24901 aaaagcgtgc cgcaaatgct ggaagatttt aaacaacacc ccgccattcg cccgctgatt
    24961 agcggcggca aactgcttga atattccgcg catatggtgc cggaaggcgg tctggcaatg
    25021 gtgccgcaac tggttaacga gggcgtgatg atcgttggtg acgccgcagg cttctgcctg
    25081 aatttgggtt ttacggtccg cggcatggat ttagccattg catcggctca ggctgccgcc
    25141 acaacggtga tcgccgccaa aaaacgcgag gatttctccg ccagcggtct ggcgcaatac
    25201 aaacgtgagc tggaacaaag ctgcgtcatg cgtgatatgc agcattttcg caagatcccg
    25261 gcgctgatgg aaaacccgcg cctgtttagt cagtatccgc gcatggtcgc cgacatcatg
    25321 aacgagatgt tcaccattga cggcaaacca aaccagccgg tacgaaaaat gatcatggga
    25381 catgcgaaga aaattgggct gatcaacttg ctgaaagatg gcattaaggg agcaaccgcg
    25441 ctatgagcca gaacgctacg gttaacgtcg acatcaaatt aggcgtcaat aaattccatg
    25501 ttgatgaggg ccacccgcat atcattctgg cggcaaatcc cgatatcaat gaattccgta
    25561 aattaatgaa agcctgccct gccggacttt ataagcagga tgacgccggc aacattcatt
    25621 ttgattctgc cggttgtctg gagtgcggca cctgtcgggt gctgtgcggt aacactattc
    25681 tcgaacagtg gcaatatccc gcaggcacct tcggtattga ttttcgctac ggctaatcat
    25741 gcatcccaca ggcccgcatc tcgggcctga tgttctgttt cgagagtcca acatgaaagt
    25801 gacattaacg ttcaacgaac aacgtcgtgc ggcgtatcgt cagcaagggc tatggggcga
    25861 tgcttcgctg gccgattact ggcagcagac cgctcgtgcg atgccagaca aaattgccgt
    25921 ggtcgataat catggtgcat cgtacaccta tagcgcgctc gatcacgccg cgagctgtct
    25981 ggcaaactgg atgttagcga agggtattga atcaggcgat cgcatcgcat ttcaactgcc
    26041 tggctggtgt gaatttaccg ttatctatct tgcctgcctg aaaatcggtg cggtttccgt
    26101 gccgctgttg ccttcctggc gggaagcaga actggtgtgg gtgctcaata agtgtcaggc
    26161 aaaaatgttc tttgcaccga cgttgtttaa acaaacgcgc ccggtagatt taatcctgcc
    26221 gctgcaaaat cagcttccac aactacaaca aattgtcggc gtggacaaac tggctcccgc
    26281 cacctcttcc ctctcattaa gtcagattat cgccgacaat accccactga ccacggcgat
    26341 aacgacccac ggcgatgaat tagctgcggt gctgtttacc tccggaaccg agggtctgcc
    26401 aaagggcgtg atgctaacgc ataacaatat tctcgccagt gagcgggctt attgcgcgcg
    26461 gctgaatctg acctggcagg atgtctttat gatgcctgcg ccactcggcc acgtaacggg
    26521 ctttctgcat ggcgtaacag caccattttt gattggtgct cgcagcgtgt tgttagatat
    26581 tttcactcct gatgcgtgtc tcgcgctgct tgagcagcag cgttgcacct gtatgctcgg
    26641 cgcaacgccg tttgtctatg atcttttgaa tttgctagag aaacagcccg cagacctttc
    26701 agcgctgcgc ttctttcttt gcggtggcac cacaatcccc aaaaaagtgg cgcgtgaatg
    26761 ccagcagcgc ggcattaaat tattaagtgt ttatggttcc acagaaagtt cgccgcatgc
    26821 ggtggtgaat ctcgatgatc ctttgtcgcg ctttatgcac accgatggtt acgctgccgc
    26881 aggtgtagag attaaagtgg tcgatgacgc acgcaaaacc ttaccgccag gttgcgaagg
    26941 tgaagaagca tcgcgtggcc ccaatgtgtt tatggggtat tttgatgaac ctgaattaac
    27001 cgcccgtgcg ctggatgaag aaggctggta ttacagcggc gatctctgcc gtatggatga
    27061 ggctggctat ataaaaatta ccggacgcaa aaaagatatt attgtccgcg gcggcgaaaa
    27121 tattagcagc cgtgaagtag aagatatttt attgcagcat cctaaaattc acgatgcctg
    27181 tgtggttgca atgcccgatg aacgtttagg tgaacgatca tgcgcttatg tcgtgctgaa
    27241 agcgccgcat cattcattat cgctggaaga ggtggtggct ttttttagcc gtaaacgggt
    27301 cgcaaaatat aaatatcctg aacatatcgt ggtaatcgaa aaactaccgc gtactgcctc
    27361 aggtaaaata caaaagtttt tgttaagaaa agatattatg cggcggttaa ctcaggatgt
    27421 ctgtgaagag attgaataag tttcatcttc ggggatcaca taaccccggc gactgaacgc
    27481 cgccggggat ttattttatt tcttcagttc agccaggctt aaccaggttt gcaccacggt
    27541 gtccgggttc agagacaggc tatcgatccc ctcttccatc aaccatgcgg caaagtcttc
    27601 gtggtcggac ggaccctgac cgcaaatccc gacatatttg ccctgtttct gcgcggcacg
    27661 gatagccatc gacagcagtg ctttcaccgc atcgttgcgc tcatcgaaca attcagacac
    27721 cacgccggag tcacggtcca gaccgagcgc cagctgcgtc atatcgtttg agccaattga
    27781 gaagccgtcg aaatattcga ggaactgctc ggccagcaag gcgttggacg ggatttcaca
    27841 catcatgatg attttcagcc cgttctcacc acgcttcagc ccctgacgcg ccagttcctc
    27901 aaccaccgct ttcgcctgat ctacggtacg cacgaacggg atcatgatct caacgttggt
    27961 cagccccatg tcgttgcgca cacgtttcac tgcttcgcac tccagcgcga agcagtcgcg
    28021 gaagctgtcg gaaacatagc gtcccgcgcc acggaagccg agcatcgggt tctcttcatc
    28081 tggctcgtag cgctcaccac cgaccaggtt ggcatattcg ttcgatttaa aatcagagag
    28141 acggacaatg acgcgcttcg gataaaacgc ggcacccagc gtcgcgatcc cttcagtcag
    28201 acgaccaacg taaaattcac gcggagaatc aaaacctttc atcatctcgc ggatttcgtt
    28261 ttgcaactgc ggttcctgat cgtcaaactc aagcagtgcg cgtgggtgga cgccaatcat
    28321 acggttgatg ataaattcca gacgcgcaag gcccacgcct tcgttcggca ggcaggcgaa
    28381 gtcgaaagca cggtccgggt taccgacgtt catcatcact ttcaacggca gatccggcat
    28441 cgtttctacg ctggagcttt tcacgctaaa ttccagcaac tccgcataga cgtaaccggt
    28501 atcaccttcg gcacaagaaa cagtgacgtt ctcaccgtct ttcatccgtt ctgttgcatc
    28561 tccacagccc actaccgccg gaatgcccag ttcacgagcg atgatcgccg cgtgacaggt
    28621 acgaccgcca cggttggtga cgatggcaga tgctttcttc atgatcggtt cccagtccgg
    28681 gtcggtcatg tcagtaacca gcacgtcgcc aggttcgatg cggttcattt cgctgatgtc
    28741 atggatgact ttcaccggac ccgcgccgat gcgatgaccg atagcacggc cttcggcgat
    28801 aatcttaccc tgtgaatgca gcgtataacg ctccatgacc tgaccgcgtg agcgcacggt
    28861 ttccggacgc gcctgcacaa tgaacagttt gccggtgtgg ccatctttcg cccactcaat
    28921 atccatcggg cgaccgtagt gtttctcaat ttgtacggcc tgttttgcca gttcctgcac
    28981 ttcttcgttg gtcagcgaga agatgtcacg ctgttcctgc ggtacgtctt cgattttaac
    29041 ctgcttgccg tgctcctggg tcggcgcgta aaccatgcgg atttttttcg accccatggt
    29101 gcgacgcacg atagccgggc gattcgctgc aagtgtcggt ttatggacgt aaaactcatc
    29161 cgggttaacc gcgccctgca cgaccatttc accaaggccc catgcggaag tgataaacac
    29221 cacctggtca aagccggatt cggtatcaat ggagaacatc acgccagaag atgcgaggtc
    29281 agagcgcacc atccgttgaa caccggcgga gagcgccaca ccacggtgat cgtaaccctg
    29341 gtgcacacga taagagatgg cgcgatcgtt aaacagagaa gcaaatacat gtttcactgc
    29401 cacgagaacg gcgtcaaaac cctgaacgtt gaggaaggtt tcctgctgac cggcaaagga
    29461 agcgtccggc atatcttctg cagtagcgga ggagcgcacc gcaaaagagg cgttttcgtc
    29521 atcggcggaa agctgtgcat aagcttcgcg gatggcattt tccagctcag gctggaaggg
    29581 agtgtcgata atccactggc ggatttgcgc gcccgctttc gcaagctggg taacatcgtc
    29641 aatatccgtt ttatccagca gttcataaat gcgctggttt acgccgcttt ggtccagaaa
    29701 ctggttaaac gcgtcggcgg ttgtggcgaa accattcgga acggaaacac ccattccgga
    29761 aaggttagta atcatttcac ccagggaggc atttttgccc ccaaccctgt ctacatcatt
    29821 catgccgagt tggttatacc aaagcaccag cggtgacgag ccattgttgg acatcgaaca
    29881 atccttttgt gataaatgaa cggtttgaga aacacatttc tgcgcattta tctttgcata
    29941 tttaaccgga tgaaaaaaac ggtgaatcgt tcaagcaaat atattttttt actttttaag
    30001 actgatccca gcgttgcgca aatctgctct cttcgacaat ttccacataa accatcggta
    30061 taaacgaaac cataaaattg aaatgctgtt ttcataaaaa ataaaattga aggttcattt
    30121 tataaaccag acataacgtt ctacgcttct gtgcgttttt aatttatgct ttcatagaat
    30181 tatgtctgca tcacgggaag aacaaaatgg ataatgctgt tgatcgccac gttttttata
    30241 tttctgatgg tacggcaata actgcggagg tattaggaca cgcagtaatg tcacaatttc
    30301 ccgtcactat cagcagcatt acgctgccgt ttgtcgaaaa tgagagccgt gcacgggcag
    30361 tgaaggatca gattgacgca atttatcacc agacaggtgt gcgcccgctg gtcttctact
    30421 ccatcgtgtt gccggagatt cgcgccatca tcttgcaaag tgaaggcttt tgccaggata
    30481 ttgttcaggc gctggttgcc ccgctacaac aagagatgaa actggatcca acgccgattg
    30541 ctcatcgtac ccatggcctt aaccctaata atctcaataa atatgatgcg cgcattgcgg
    30601 cgattgatta caccctcgcc cacgatgacg gcatttcgtt gcgcaatctg gaccaggctc
    30661 aggtgatcct gctcggtgtt tctcgctgtg gtaaaacccc caccagtctg tatctggcaa
    30721 tgcagtttgg tatccgcgcg gcaaactacc cctttattgc cgacgatatg gacaatctgg
    30781 tgctacccgc gtcgctcaaa ccgcttcagc ataaattgtt cggcctgact atcgacccgg
    30841 aacgtctggc ggcgattcgc gaggaacgtc gggagaacag ccgctatgcc tcgcttcgtc
    30901 agtgcaggat ggaagtcgcg gaagtagaag cgttgtaccg taaaaatcag atcccgtgga
    30961 ttaacagtac caattattcg gtagaagaga ttgccaccaa gatcctcgat atcatggggc
    31021 ttagccgccg aatgtactag agaactagtg cattagctta tttttttgtt atcatgctaa
    31081 ccacccggcg aggtgtgaca cacctcgcac ttgaaatcag ctgcgattgg tttatcgtga
    31141 tgcgcatcac ttcccggtag tcctgccgta gaagcaacaa atttctgaga cttgtaatga
    31201 acagaactga cgaactccgt actgcgcgta ttgagagcct ggtaacgccc gccgaactcg
    31261 cgctacggta tcccgtaacg cctggcgtcg ccacccatgt caccgactcc cgccgcagaa
    31321 ttgaaaaaat actgaatggt gaagataagc gactgttggt cattattggc ccctgctcga
    31381 tccacgatct caccgctgca atggagtacg ccacccgtct gcagtcgctg cgcaaccagt
    31441 accagtcacg gctggaaatc gtaatgcgca cctattttga aaaaccacga actgttgtcg
    31501 gctggaaagg gctaatctcc gatccggatt taaacggcag ctatcgggta aatcacggtc
    31561 tggagctggc gcgcaaatta cttttacagg taaatgagct gggtgtccct accgcgactg
    31621 agttcctcga tatggtgacc ggtcagttta ttgctgattt aatcagttgg ggcgcgattg
    31681 gcgcacgtac taccgaaagt cagatccacc gtgaaatggc ttcggcactc tcctgtccgg
    31741 taggttttaa aaatggtacc gatggcaaca cgcggattgc cgtggatgct atccgcgcag
    31801 cccgcgccag ccatatgttt ctctcgccag acaaaaatgg tcagatgacc atctatcaga
    31861 ccagcggcaa cccgtatggc cacattatta tgcgtggcgg caaaaaaccg aattatcatg
    31921 ccgatgatat cgccgcagcc tgcgatacgc tgcacgagtt tgatttacct gaacatctgg
    31981 tggtggattt cagccacggt aactgccaga agcagcaccg tcgccagtta gaagtttgtg
    32041 aggatatttg tcagcaaatc cgcaatggct ctacggcgat tgctggaatt atggcggaaa
    32101 gtttcctgcg cgaaggaacg caaaaaatcg tcggcggtca gccgctcact tacggtcaat
    32161 ccattaccga cccgtgtctg ggctgggaag ataccgaacg cctggtcgaa aaacttgcct
    32221 ctgcggtaga tacccgcttc tgaatgcgtg cccattcctg atgggatggg catttctgcg
    32281 caacttgttg tcttctcagc acattactgc ttgctctggt cagccataat attgataata
    32341 agaatcattg ttatatcaat tattattaat ttttatgcgt tatacggata gcagaaaact
    32401 cacgcctgaa acggatgcca atcacaagac cgcttccccg cagcctattc ggcgaatttc
    32461 cagccagaca ctgttaggtc cggatggcaa actgattatc gatcatgacg ggcaagaata
    32521 tctgctccgt aaaactcagg ctggcaagct gctgttgacc aagtagcctt taactcgagc
    32581 agctgacttc cagccgttta ccccagtcag gtggacgact gacatagtca tcagctctgt
    32641 cgctgaaagg atttcgcaac gcctcatgca ggcggtgcaa ttccgtcata tcaccctttt
    32701 ctgccgcttc aatcgcccgt tgcgccaacc agttgcgcaa caccagagct gggttaacgc
    32761 tttgcatcag ttgctgacgc tcaatatcgc taacctcgtc ttgttgcaaa cgcctccgat
    32821 aacgggcaaa ccagtcatca aatgccgcac gatcaataaa ctcatcacgt agcggtgacg
    32881 ccgcgctgtg ctgctcggtc agactcagca tgcggaatgt gcgggtataa tcgctgcgct
    32941 ctcgcgccat cagactgaat aattcattca gtagcgcgtt atcctctttt tgctccgtca
    33001 tgaagcccag tttctgccgc atccgttgtc cataatgcgt caacaaaacc tgctgatagc
    33061 tgtccagggc ctcattcagg gcatctacgg caacaaatgg tgacaatgtc tgcgccagac
    33121 gctgtaaatt ccacaacgcg acagcaggtt gattatcaaa gctataacgc ccttgatgat
    33181 ccgagtgatt acaaataaaa ccgggttcgt aatcatcaag aaaaccaaac ggcccgtaat
    33241 caagcgtcag ccccagcagc gacatgttgt cggtattcat caccccatga gcaaagccga
    33301 ccgtctgcca ttgggcaatt aacgatgcgg tacgtgcgac aacatcggta aaccagagac
    33361 ggtatttgtc ctcatcatct gcaagatgtg accagtaatg acgaatggca aagtcagcta
    33421 actgacgaac cttttccggc tcgcggcgat agtaaaaatg ttcgaaatga ccaaagcgca
    33481 gatgacttgg tgccacacgt atcagcatcg cgcctggctc caccgtttcc cgatacactg
    33541 gcgaatcgct ggtgacgata cttaacgcgc gggtcgtcgg aatgcccaga taatgcatcg
    33601 cctcactggc gagactttct cgtatcgtcg aacgtaaaac cgcccgtcca tcacccattc
    33661 gcgaataagg cgtcaggcca gcacctttca gatgccagtc cattgtagtg ccatcagcaa
    33721 gcagttgttc gccaagtaaa atgccgcgcc catcacccag ttggcccgcc cagacgccga
    33781 actgatgacc actgtaaacc tgcgccagtg gtgacatacc aggcagtagg gtttcgccgc
    33841 cccagacacc tgcagcattt ttaaacagcg acgatggaat actcagcgtg ttagcgagtt
    33901 cggtattatg ccaaatcagc cgggcattat ttaaaggcgt aggggaaagt gctgtatagg
    33961 tttctggcaa ttcatcgcgc cagcgggtaa taaaagacag ggtcatagat cctcctgttt
    34021 gatagtgtag acggttactc tcgtcttaaa caccagcaaa caggagggtt atcgctgaac
    34081 caacgtcgtt atctgattta taggaacggc aggccacagg catccctgga gagcatggaa
    34141 atcgaagggg gtaatctgtg ccaatatttc tgccgtatca atgcctcccg cgatgatgca
    34201 attgcaacaa ggggaaattt gcgcctggat cgcacgaata aacggttcga aggagcggtg
    34261 cgtgatttgt tgctgaataa aacttttatc cagcatgacg cgggtgaaaa gaccatcaaa
    34321 aaccgctttc attgtgctat tgcctgctcc caaattacct aacacaaggg gatacacttg
    34381 cgataaggat aaaagaccgc gattatcttt tccttcattg agatgcgggt aattctcatt
    34441 aatcaacagt tcaataaacg gatattttaa tagttcgcct gcgtaattat ccctctctaa
    34501 taataatgta gccacttgtg gcgttaaatt aagccaggca aagagtttat gttgaataaa
    34561 aaaatgttgg catgatttta gtaattccaa ctgctctgaa aataattgcc aatgttgctc
    34621 ctcggtaagt tgcgctataa cccgactcgt cgggatacgc accgtgccat cttcactgga
    34681 gaaatgggta attaactcaa cgccgacaag gagttgctgg ttatctctta tcggcaggaa
    34741 atagcaatca gaatgataaa gattctccaa aaaaatcttc atggtaaccg tccctctcga
    34801 aggatgtttt tcagtatccg gctaaaaggg atgaggccat aagacataac aattacagaa
    34861 ggagtaactt tcatttgttc catgttaacc actttttcag gggtcctttt tttagattat
    34921 cctgattata aacgaataat ccatttacgg aatttttgtc tgcaaaatac tactgtattc
    34981 aggagtaaat ggacggcgat gtatcatcgc cgtccatttt tcgacatact agattcgtct
    35041 cgcctgccag aaatttttct gccaatagac attatcaagt gaggaacgca tcactccctt
    35101 gctggtagag gcgtggataa attggttgtt ggtatcgtaa atacctacat gcaaaccatt
    35161 ttgtccggac cccgttttga aaaagaccag gtcacctggc agcaactcgt ctttatcaat
    35221 ttgcgtgcca atagaggctt gttgtttggt ttcacggggc agctgcaaat cgaaacgatc
    35281 gcgcatcgtc acaaccacaa atcccgaaca gtccacaccg cgccgcgtca tgccaccata
    35341 acgatacggc gtgccatgcc agctttgtag ctggtcgttc aaaccggcaa taacggtaat
    35401 cgaatcagaa agtctggcat ttggcggcgg tgctttatgg tggctacacc cggccagaaa
    35461 cagtgctgtg atcaaaataa ggcagaaacg cattccgtac ggttcctctg ttttttattc
    35521 ttgcattaat ttagcgtcgt aattacccga ttttcaagat actaatgaaa tcagatggtc
    35581 gaaatcagca ttctgtgacc ttcgatatcc agacggcgaa aattcatccc ataggcctgc
    35641 gccagatttg gcggcgtgag cacctcttct ctgcgtccac tggccagcat ttttccacct
    35701 tttagcaacc acgcccgatg cgcatgacgc aacgtgtggt tgagatcgtg actgctcatc
    35761 acaatcgcca gtccttgctg acacagcgcg ctcagaattt tgtctaacgc actttgttgc
    35821 gcaacatcaa gactgttcat cggctcatca agaagcagca attggcctgc gggattggct
    35881 tgtggtgtga tttgcaacac caccgcagca agacgtacgc gttgccattc accgccggaa
    35941 agttgattgg tgctacgacc gagtttgtca tcaagagcca gcgcccctgc gacatcattc
    36001 agtagttcgg tacgcgtttt atcgtgctga tgcagtgtca ggtagtgcca gaccggcatt
    36061 gcaaacggcg gcgtctgctg ttgtgaaaga taggcgcgat gcagcgcgag ttttgttgcg
    36121 gaccatgctt ccaggggttg ccccgcgaac tgaatgcttc ccttaccgct ggtcattccg
    36181 gccattcgtg caagcaaggt actcttaccc gcgccattcg gccccaccag gtgcaggatc
    36241 tccccagccc gaacctcgcc agaaagtggc cccaggcggg tagattccgc aacatcctgt
    36301 aactgcatca caatagacat tattttgcca acgccagttt aatgctttcc atcacaatgg
    36361 ggtcttccgg cgtcatatcc ggggaaaaac gctggatgac caatccgtcc ctgccaacta
    36421 aaaatttctc aaaattccat aaaatatcat cagggtacag cggtgcacga cctttgctga
    36481 ccatacgggc atagaatccg ctctcttccg gcgcgactgc ggtcggcgct gcggcaatca
    36541 atttttgata cagcggatgg cgtccttcgc cgttaacttc aatcttactg aacatcggga
    36601 acgtcacccc ccatgtggtg gtacagtaag ttttaatctc ttcatcgctg cccggttctt
    36661 gttccagaaa ctggttgcac gggaatccca gcaccacaaa acctcgatcg gcccaggctt
    36721 tctgaatatt ctccaactgc tcatattgcg gcgttaagcc acactttgag gcgacattga
    36781 caatcaacag cacattaccg gcgtacttct ccagcgtggt cacttcaccg tcgatatctt
    36841 tcactacggt cgtcagaatg gaatcttgca tcgtttctcc tgggtgtggt cagtaaaaat
    36901 cttagctttt aatcatagac cgtctttttg cggctaacgt cttgctttta acaataacca
    36961 gataaacacc ggcgcaccca acgttgcggt gaccacgcca ataggcagct ctgcggcagc
    37021 taatgccagg cgcgctacaa tatcggccag cagcaatgcg ctcgcccctg ccagcgcgca
    37081 gccgggaagt aatacgcgat gatcggttaa accacacaac cgaagaatat gtgggatcac
    37141 cagaccaata aagccgatag cacccgccag cgccacactg acgccaacca tccagccggt
    37201 cgcggccacc agcacattgc gccagaacca caggggtaaa cccagttgcc gcgccgagat
    37261 ctcgccaagt actaacatat tcatcggcct ggactgacaa cagatccaca acaacacggg
    37321 gatcaatgcc agcatcagcc agctttgccg ccagtctacg ccgccaaaac cgcccatcat
    37381 ccagtacatc agctgacgta aatcaacaga tgtggaaaag tagatagccc acgtcattag
    37441 tgcgctacag ataatcccta atgcaacgcc agccagcaat aaccgactgg tcgaaagatg
    37501 acgacgggcg aaacgtaaga gtattaaagt gatgataagc gcgccagcaa tcgcacacag
    37561 ccctagcgcc cagttgggga gttgcccttg cccaagcaat accgcggcga taagccccac
    37621 gcctgcgccg ttagagacgc caagtagtcc aggttctgcc agaggatttt caaacaacgc
    37681 ctgcattaca gcgccggata tagccagcgc cgcaccaacc agcaatacag ccagcgtacg
    37741 tggcaggcga atttgccaga cgaacagttc gccacgagga gtaaaccagt cacctggcga
    37801 gatccattgt tcaccggcgc aaaggcttaa gagaagcgcc agcagcatca aaactgacag
    37861 gcataataac cagcgaatat tttgtcgctg ttgttggcgg gcaagtgtca gcatggtatc
    37921 cgttctgctg aagtgtcatg gcgttgattt tacggtgact cttcgacagt gaaaagaaaa
    37981 aaggccgcag agcggccttt ttagttagat cagattactc gtctttgggc gaagcgtttt
    38041 cgacccggct ttttaacttc tgcccgggtc tgaaggtcac cacgcgccgt gctgtaatgg
    38101 gaatatcctc gcccgttttc gggttacgtc ccgggcgttg attcttatca cgcagatcga
    38161 agttaccaaa accagagagt ttcacctgtt cgccgttttc cagagcgcga cggatctctt
    38221 cgaaaaacag ttcaaccagt tctttggcat cccgcttgct aagcccaagc ttatcaaaca
    38281 gatattctga catttcagct tttgtaagcg ccataggttc aatccctcaa tgatgcctgg
    38341 aatcgctctt ttaatgcctc tacacatttg gcgacggtag cggcaatctc ctcttcttcg
    38401 agtgtacggc tggtatcttg caggatcagg cttatggcga ggctcttata cccctccgca
    38461 acacccttac cgcggtacac gtcaaataag tttacgccaa ctacctgatt tacgccaact
    38521 ttcttacatt cggataaaat atccgctgcg ggaacgtttt ctgcgaccac caccgcgatg
    38581 tcacgacggt tcgccgggaa gcgagaaatc tcgcgcgcct gaggcaccac gcggtctgcg
    38641 agcttgttcc actccagttc gaacaccaga gtgcgaccgt taagatccag tttacgttcc
    38701 agttcaggat gaacaactcc aacaaaacca atacgttccc ctttcagata aatcgctgcg
    38761 gattgccccg gatgcagtgc cggattcgct tctgcacgga actcaacctc attcagttta
    38821 ccggtcaggt cgagaacgga ttcaagatcg cctttcaaat catagaaatc aacggtctct
    38881 tttgccaggt tccagtgctc ttcgtaacgg ttaccgcaaa tcacaccggc taacatcaga
    38941 tcctgacgaa tgcccaacgg tgcctgagta tctggtacga aacgcagacc gctttcgaaa
    39001 atgcgcacac ggttctgctg acggttctgg ttgtacacca cggttgccag caggccggtc
    39061 cacagagaaa gacgcattgc tgacatttca acagagatcg ggcttggcag cagtaaggct
    39121 tcaacgcctg gatggatcat ctgctgcact ttcggatcaa cgaagctgta ggtgatcact
    39181 tcctgatagc ctttgtcgtt gagcagcgtt ttcacgcgct tgagcgacag gtcagcttca
    39241 cggtgagtac ccataatcag gcttgcctgt accggctcat ccgggatgtt gttgtagccg
    39301 taaacacgcg cgacttcttc gaccagatct tcttcaatct ccatatcgaa acgccagctc
    39361 ggcgcaactg cctgccactc gtctttgcct tcggtcactt cgcagccgag acgacgcaga
    39421 atgtcagtta cctgttcatc agcaatatga tggccgatca ggcgatccag tttgctacga
    39481 cgtaaagtga tggttgcacg cttcggcagc gttgcttcgt tggtgatatc aattaccgga
    39541 ccagcctcac caccgcagat gtcgatcagc agacgggtcg cacgttccat cgctttgtgc
    39601 tgcagtgccg gatcaacgcc acgctcataa cggtgagacg catcagtatg caggccatga
    39661 cgacgagcac gaccggtgat agacagcggg ctgaagaaag cgcattccag caagacgttt
    39721 tgcgtttcgt cattcacgcc agagtgttcg ccaccaaaga tgccgcccat cgccagcgcc
    39781 ttgttgtggt cggcgatgac cagagtgtca gcattcagct tcgcttcagt gccatcaagc
    39841 agcaccagcg tttcgccctc ttttgccatc cgcaccacaa tgccgccttc aatgcgatct
    39901 ttatcgaaag cgtgcatcgg ctggcccagt tcgagcagta catagttggt gacgtcaaca
    39961 actgcatcga tagaacggat cccgcaacga cgcagttttt ctttcatcca cagcggagtt
    40021 ggcgctttaa cgttaatgcc ttttaccaca cggccaagat agcgcgggca ggcttcaggc
    40081 gcttcgactg taatcggcaa cgtgtcgtcg atggtcgcac caaccggaac gatttccggt
    40141 tcaaccagcg gcagctgatt cagcacggca acgtcacgcg caacaccaat gatacctaag
    40201 cagtcggcac ggtttggcgt tacgctgatt tcgatggtgt tgtcatcgag tttcaggtat
    40261 tcgcggatgt cagtgccaat cggcgcatct gcaggcagtt cgataatgcc gctgtgatcg
    40321 tcagaaattc ccagttcaga gaaggagcac agcatccctt cagacggttc gccacgcagt
    40381 ttcgccgctt taattttgaa atcacccggc agaacagcac caatggtagc taccgccaca
    40441 cgcaggccct gacggcagtt tggcgcacca cagacgatgt ccagcaggcg atcgccgccg
    40501 acattcactt ttgtcacacg cagtttgtca gcgttcggat gctgcgcaca ctcaaccact
    40561 tcaccaacga ccacgccgtg gaagctaccg gcaaccggtt ctacaccgtc aacttccagg
    40621 cccgccatag tgatttgatt cgccagcgca tcgctatcaa tcgccgggtt cacccattcg
    40681 cgtaaccaca gttcactgaa tttcataatc tattcctgcc ttatttaaac tgtttgagga
    40741 aacgcagatc gttttcgaag aatgaacgca ggtcggtgac gccgtaacgc aacatagtca
    40801 gacgctccat ccccatcccg aaggcaaaac cagagtaaac ttccgggtcg atgccaacgt
    40861 tacgcagcac gttcggatgc accatcccgc aacccaacac ttccagccat ttaccgtttt
    40921 tacccatgac gtcaacttct gcagaaggct cggtgaacgg gaagtaggaa ggacggaagc
    40981 gaatctgcaa atcttcctca aagaagttac gcaggaaatc gtgcagagtg cctttcaggt
    41041 tggtaaagct gatgttggta tcaacaatca gaccttccat ctgatggaac atcggcgtgt
    41101 gagtctggtc gtagtcgtta cgataaacgc ggccaggcgc gatgatacga atcggtggct
    41161 gctgggcttt catggtgcgg atctgtacgc cagaggtctg ggtacgcagc aggcgggtag
    41221 tgtcaaacca gaaagtgtcg tggtcagcgc gcgccgggtg gtgaccagga atgttcagag
    41281 catcgaagtt atgataatcg tcttcgattt ccggcccggt tgccacggta aagccaagct
    41341 caccgaagaa actttcgata cggtcgatgg tacgggtaac cggatgcaga ccgccgtttt
    41401 caatgcgacg acctggcagg gagacatcaa tcgtttccgc cgccagacgc gcattcagcg
    41461 ctgcgctttc cagttccgct ttacgcgcat tcagcgcctg ctgaacctgc tctttcgctt
    41521 cgttgataac cgcaccagct gccggacgct cttctggcgg cagctcacgc agggtcgtca
    41581 tctgaagggt taagtgccct tttttaccca aatattcgac gcgcacatta tctaacgcgg
    41641 caacatctga cgcctggcta atggccgcct tcgcactggc aaccagttct gcgagatgtg
    41701 acatggtttt cctcattgtg tcagtggtga cactggttcg ttggacttag agcctatccc
    41761 atcaggctat tttacttgcc attttggtcc cgggcagtgc tcgaaattct cacgtactta
    41821 aatacgctcc ggtttctccg cgctggccgt gtccagactg gctgcgacaa ttacgcctga
    41881 tagactggct ttttattttt caaaacgcgc atacaaaaaa agcctccact gggaggcttt
    41941 caggcgctgt tttccgtttc tcttctcacg cactagcctc ctggattcag gtgctaaagt
    42001 aaaaaaagaa gcggaaaata gcagcattca ttgcttgcgt taccttttgg tactcttcaa
    42061 aagaccttta ttgaaaaggc tacggcgata aaagtcaatg ttttgatggc gttgaaacga
    42121 aaagagggag actagctccc tctttcaact ggcttatgcc agagctgctt tcgctttttc
    42181 aaccagagcg gtgaacgcta ctttgtcgaa tactgcgata tcagccagga tcttacggtc
    42241 gatttcaaca gaggcttttt tcaggccatt gatgaatttg ctgtaagaaa taccgttctg
    42301 acgtgctgct gcgttgatac gcgcaatcca cagttgacgg aactgacgct tacgttgacg
    42361 acggtcacgg taagcatact gaccagcttt gataacagcc tggaaggcaa cgcggtatac
    42421 gcgagaacgc gcaccgtagt agcctttagc ttgtttcaaa attttcttgt gacgtgcgcg
    42481 tgcaataaca ccacgtttta cgcgagccat atgtgctctc ctgtatctat attctaatta
    42541 aaaagttaaa aacgttaacg gcttatgcgt acggcaggca cgcgattacc aggcccagat
    42601 cgcctttgga aaccatggct ttcggacgca ggtgacgttt acgtttggtc gcttttttgg
    42661 tcagaatgtg acgcaggtta gcgtgcttgt gcttaaaacc acctttaccg gtttttttga
    42721 agcgcttagc agcaccgcgt acggtcttaa tttttggcat tttaataact tccacttcgc
    42781 attgttaata aacgaaacaa aggcgaacaa agcctgtgaa gcccgaaggc tccacagaca
    42841 gtgctacttg aaggccttac tgtttcttct taggagcgag caccatgatc atctggcggc
    42901 cttcgatctt cgttgggaag gattcgacca ctgccagttc ttgcaaatcg tctttcacgc
    42961 gattaagcac ttccataccg atttgctggt gcgccatctc acgaccgcgg aaacgcagcg
    43021 tgattttggc tttatcaccc tcttcgagaa agcgaatcag gctgcggagt tttacctgat
    43081 agtcgccttc atctgtacca ggacggaatt taatttcctt aacctggata actttttgct
    43141 ttttcttctg ttccttagaa gacttgctct tttcatagag gaatttgccg taatccatta
    43201 tacgacaaac cggcggctcg gcgttagggc tgatctcgac taagtctact ccggcttctt
    43261 ctgctttctc cagagcttct ctcagactca caataccaag ctgctcgcct tccagacctg
    43321 ttaagcgaac ttcctgggcg cgaatttcgc cattgatacg gttagggcgc gccgtttgaa
    43381 ctcgttttcc gcctttaata ccttattcct ccaattgttt aagactgcgg ctgcgaatct
    43441 cttgttgcag cttctcgatc acttcattta cgtccatgct tcccaggtct ttaccacggc
    43501 gggtgcgaac ggcaactttg cctgattcca cctctttatc accacagacc agcatatatg
    43561 ggacgcgacg caaagtgtgc tcgcggattt taaagccaat cttctcattt ctcaagtctg
    43621 ctttaacacg aatgcccgca tttgatagtt tttgcgtcaa ttcgttaacg tattcagact
    43681 gtgaatcggt aatattcatg ataacaacct gaactggcgc aagccaggtc gggaagaaac
    43741 cagcgaattc ttcggtcagg ataccgatga aacgttccat cgaccccaga attgcgcggt
    43801 gaatcattac cggtacctta cgctcgttgt cttcgcccac ataggaggcg ctcagacgag
    43861 acggcaagga gaagtccagc tgtactgtac cgcactgcca tgcacgatcg aggcagtcat
    43921 acagggtaaa ttcaattttc ggaccgtaga aagcgccttc acccagttga tattcaaacg
    43981 ggatgttgtt ttcttccagc gcaaccgcca agtccgcctc agcacgatcc cacatttcgt
    44041 cgctgccaat acgtttttca ggacgagtgg agagtttgac gacgatcttc tcgaagccaa
    44101 aagtgctgta catatcatag actaaacgga tacatccgtt aacttcatcg cgaatttgtt
    44161 cttcagtaca gaagatatgc gcgtcatcct gggtaaatcc acgcacgcgc atcaggccat
    44221 gcagcgaacc tgacggctcg ttacggtggc agctaccaaa ctcggccata cgcagcggca
    44281 gatcgcgata agacttcagc ccctggttga aaatttgtac gtgacccggg cagttcatcg
    44341 gcttaatgca gtattcacgg ttctcagaag atgtggtgaa cattgcatct ttgtagttgt
    44401 cccagtgacc ggttttttcc cacaggacac ggtccatcat gaacggacct ttaacttcct
    44461 gatactggta ctctttcagt ttagaacgaa caaacacttc cagttcacgg aagatggtcc
    44521 agccgtcgtt gtgccagaat accatacccg gcgcttcttc ctgcatatgg tacaggtcga
    44581 gctgtttacc gattttacgg tggtcgcgtt tcgcggcttc ttccaggcgc tgcaggtaag
    44641 cgttaagtgc ttttttgtct gcccacgccg taccgtaaat acgttgcaac attttgttgt
    44701 tgctgtcgcc acgccagtaa gcccctgccg ttttcattag tttgaaatga tggcagaaac
    44761 gcatgttcgg tacgtgcgga ccgcggcaca tatcgacata ttcttcatgg aagtacagac
    44821 ctggcttgtc atcatgggcg atgttttcgt caagaatgga gactttgtag ctctccccac
    44881 ggttggcgaa agtttcacgc gcttcgtgcc agctgacttt cttcttaatg acgtcgtagt
    44941 ttttctcagc aagctcatgc atccgcttct cgagtgcttc gacatcttcc tgggttaacg
    45001 tgcggtcaag atcaacgtcg taataaaaac cgttgtcaat aaccgggccg attgccattt
    45061 tggtatgcgg ccaaagttgt ttaatcgcat gccctaacag gtgcgcacag gagtgacgaa
    45121 tgatctccag accttcttcg tctttggcgg taatgatcga cagttgtgcg tcgttttcaa
    45181 tcagatcgca agcatcaacc agttcgccat taacgcgccc tgcgatacag gctttcgcca
    45241 gacctgggcc aatgtccagc gcaacatcca tggggcttac agcgtggtcg taatggcgtt
    45301 ggctgccatc aggaagagtt ataacaggca ttttatatcc ttatttgcag tggtgaccca
    45361 cacgaaagat cacatacaaa gaaaaatttg tttattaaca gttcattgcg aaaccatcta
    45421 gccaacaaat gctaaattgg ttcgcaatca ggtacacaaa tgaaagacat taacctctca
    45481 ccttccacct ggttgaacga tgttaacact aaaaaaaggg agattgtacc tttccgtttc
    45541 acattgattc gattcgcaat tcgtttgtag tgagtacacc agcaaaacgc ttaaagaacc
    45601 actgataaca tcacggtgcg tctgaaaggt ttactgcgag cggatttaca gcagcataaa
    45661 ctgaatccag caaagttcgt tgcgtgattt ggcggtagta ttttaaacgc tcatgagatt
    45721 aatgacgaag tggtcatatc acaatgataa aagtgacaca attcttatag caatttttcg
    45781 tgcacatttc gttctggcga taataattaa tcatcatcct caaaacctcc agacatatat
    45841 aaggcgaata gattatgagt caaaacgata tcattatcag aactcattat aagtctcctc
    45901 atagattgca catcgatagc gacataccaa cgccttcatc agagcctatt aatcaatttg
    45961 cgcgccaact catcacccta cttgatacct ctgacttaag ttcgatgctg tcatactgtg
    46021 ttactcagga atttaccgca aactgtcgaa aaatttcaca aaattgttat tccactgccc
    46081 tttttatcat taactttgcc acttcaccca tccatgcaga aaatatactc attacattac
    46141 actataaaaa agaaatcatt cccttatttc tggaaaccac gcctattaag gctaaccatt
    46201 tgcgaagcat actggattat attgaacagg aacagttaac tgccgaagat cgtaaccatt
    46261 gtatgaaact gtctaaaaaa atccatagag aaaaaactat acaaccaaca gtaaatctca
    46321 atggtagtgc atttttttcg caatctcctt ctgacgctat tttttgtcgc catctgtcat
    46381 tgcaatacgc ccttgattca ttgagaaatg gaaaaggcaa agtcaacctg attaaacact
    46441 actcctccgt tgaatccata cagcagcatg tccccttagt ccgggacgcg gagttcagag
    46501 cattacttcg ccatcctcct gcagggagtc gcgttatcgc gagtaaggat tttggcttcg
    46561 ctttagatat tttcttttgt cgaatgatgg caaacaatgt cagtcatatg tccgcgattt
    46621 tatatataga caatcatact ttgtcagtaa ggctacgaat aaagcagtca gtgtatggac
    46681 aattaaatta tgttgtgtct gtttacgacc cgaacgatac caacgttgcc gtcagaggca
    46741 cccacaggac agcacggggc tttctctcgc ttgataagtt catcagttca gctcccgatg
    46801 ctcagacctg ggctgatagg tatgttcgca actgtgcaat tgctattctt cctctattac
    46861 ctgagggagt tccagttgct attttagcgg gtatcacgac acgaatgcca tttgccccta
    46921 tacatccatc ggcaatgttg tcaataatgg ccacaggcca gactcaatag cttattacac
    46981 tattcaaaca gttgcccata ctccctgaaa aagaaatcat tgaaataata actgcgcaga
    47041 atagcgttgg tacacctgct ttatttctgg ctatgatgaa cggacaaact gacaacgtaa
    47101 aaatatttat gcaagaaatt cagtcactgg tatacaatca tatcattcat gaagataatc
    47161 tggttaaatt actgcaaact aaaagtgcta acgaaacacc tggactctat atctccatgt
    47221 tgtatggatt cgatgaaata atcgatatct ttctgaatgc attaaccact ccaatagcac
    47281 aagaactttt aaacaaaaaa tggtgatgag tattttagcc atgaaaatac atgatggtga
    47341 gccaggatta tacgccgcaa tggaaaataa tcaccctttg tgtgtcacac ggttcctctc
    47401 taaaattaat ggcatcgcct ttaaatacaa gttgagcaaa gctaacatca tggatttatt
    47461 aaaaggagct acagcacagg gaacccctgc attatacatc gccatgagca agggtaatga
    47521 agacgtcgtg ttatcttata tatcgacgct gggtgctttt gcaaaaaaac attcttttag
    47581 tcaacatcag ttatttacac tattggccgc taaaaatcat gacaacatgt cagctgttca
    47641 tatagccatt catcataagc attataaaac tgtagaaaca tattatgctg ctataaatgt
    47701 catcagccaa agcctgagtt ttagtgctga tgaaataaag acgtatttat aaccagccat
    47761 ttacgatata tatcgataca taattttatt tttttcataa aacgcagaaa cagtgaccat
    47821 gtgcacaacg ccacccgacg gctgtaataa caaaaaatcc cttgatgcct atcccttttg
    47881 ttacactccg ttatcacgca caagagatat gcaggacact ggtatgccga ctaaacgctt
    47941 tgataaaaaa cactggaaga tggtggtggt gctactggca atctgtggcg ctatgttgtt
    48001 gctacgttgg gctgcaatga tttggggctg agcagtgaca aacgaagaca gccagaccgc
    48061 ataacacggt ccggcagata attttacatg ctgtagccca gcgataaggt tgtccgacga
    48121 tcggtatgtt ccggtgcaga ctctggaggt tcagagttcc atgtgacgtt gtaagccacc
    48181 ttcagtccaa aatgttcatt aatagcaaca tttaatgcgc tttcggagtt cagcgttgta
    48241 tcttccgcgc caaagaccga aacaccctgc gtaaatttag cgttgtcagt caactgccat
    48301 gcataagccc cggaggcata gcccagcggc tgggtttcac tggcattgtc ggtatatttg
    48361 tcgtaacgca cacctgggcc gaattcaaag cggaaactgt gtaccgggcc attcaaaaac
    48421 tggcgaccat aacccgcggt caacacatcg cgctctcgat agccgttata acggtctgtc
    48481 agccagcttg cctgaccaaa taaatagtca taatcagtta aattaaaacg gctacgcccg
    48541 cccgccgcat atttttctga agaacgctca tcattagaag aagtattact ggcgttcccc
    48601 cacagcgacc aggcagtggt ttgtccatac caggtcatgg tggtgtcagc cgtaagtgag
    48661 gagcttttcg tattgcctga ttgtgcaaga tatcctgcgt tcagattacc ttcgaaaggt
    48721 tttttagcgc tggcaggatc gtccatgaca gtaaaaacgg aatcatcggc agctgcattc
    48781 agtgacgcaa acatgccccc cgccaacata acgatggcag gaactgtctt caaaagcttc
    48841 atttatcaag agtccgtaca acaaaaaaag agaccatcgc ggtcccggaa actttcttaa
    48901 ggatcaaaga ttagcgtccc tggaaaggta acgaattata aaaaggcgcg aataacttag
    48961 caatgtattc ttatttcatt ttttggaata agtatgtggc gaaaacagat ttttatttat
    49021 atatatttat ctgcaaaatt ttaaataaag ctccaataaa tcatattgtt aatttcttca
    49081 ctttccgctg attcggtgcc agactgaaat cagcctatag gaggaaatga tggtacgtat
    49141 ctatacgttg acacttgcgc cctctctcga tagcgcaaca attaccccgc aaatttatcc
    49201 cgaaggaaaa ctgcgctgta ccgcaccggt gttcgaaccc gggggcggcg gcatcaacgt
    49261 cgcccgcgcc attgcccatc ttggaggcag tgccacagcg atcttcccgg cgggtggcgc
    49321 gaccggcgaa cacctggttt cactgttggc ggatgaaaat gtccccgtcg ctactgtaga
    49381 agccaaagac tggacccggc agaatttaca tgtacatgtg gaagcaagcg gtgagcagta
    49441 tcgttttgtt atgccaggcg cggcattaaa tgaagatgag tttcgccagc ttgaagagca
    49501 agttctggaa attgaatccg gggccatcct ggtcataagc ggaagcctgc cgccaggtgt
    49561 gaagctggaa aaattaaccc aactgatttc cgcttcgcaa aaacaaggga tccgctgcat
    49621 cgtcgacagt tctggcgaag cgttaagtgc agcactggca attggtaaca tcgagttggt
    49681 taagcctaac caaaaagaac tcagtgcgct ggtgaatcgc gaactcaccc agccggacga
    49741 tgtccgcaaa gccgcgcagg aaatcgttaa tagcggcaag gccaaacggg ttgtcgtttc
    49801 ccttggtcca caaggagcgc tgggtgttga tagtgaaaac tgcattcagg tggtgccacc
    49861 accggtgaaa agccagagta ccgttggcgc tggtgacagc atggtcggcg cgatgacact
    49921 gaaactggca gaaaatgcct ctcttgaaga gatggtccgt tttggcgtag ctgcggggag
    49981 tgcagccaca ctcaatcagg gaacacgtct gtgctcccat gacgatacgc aaaaaattta
    50041 cgcttacctt tcccgctaac aaaaacattc ccccagcatt gggggaatca ccaccaacct
    50101 gtcggcaacg cgtttctccg actatgctca aaagtcatgt gataacaaag gggtgaacta
    50161 tggccagtgg cgatcttgtc cgttatgtca taaccgtcat gttgcatgag gatacattga
    50221 ctgaaattaa cgagttgaat aattacctga ctcgcgacgg ttttttgctc accatgacgg
    50281 atgatgaggg aaatatccat gagctgggga ctaacacttt tggacttatc agtacccaaa
    50341 gtgaagaaga aattagagaa ctggtttcgg ggcttaccca aagtgcaacc ggcaaagatc
    50401 ctgaaatcac catcacgacc tgggaggaat ggaatagcaa cagcaaataa atggtttttg
    50461 ggcaataatc agtctgtggt gtgcgttagc tcgtgttttt acaccgcatt cttgcgctaa
    50521 ccttatgatc tggcagacaa catgggagag acatcatgtg gcaggcaatc agtcgtcttt
    50581 tgagcgagca gttaggtgaa ggcgaaatcg aactgcgtaa tgaactgcct ggcggagaag
    50641 tccatgccgc atggcatttg cgctatgcag gacatgactt tttcgtcaaa tgtgatgaaa
    50701 gggaactgct tcccggtttt accgccgaag ccgaccaact ggagttactg tcgcgtagta
    50761 aaaccgtcac cgtgcctaag gtttgggcag taggcgctga ccgtgactac agttttctgg
    50821 tgatggatta tctcccacct cgtccgctgg atgcgcatag cgcatttatt cttggtcagc
    50881 aaattgcgcg tttacatcaa tggagtgacc aaccacaatt tggcctcgat tttgataact
    50941 cactctccac aactccacag cccaacacct ggcaacgtcg ctggtcaacg ttttttgctg
    51001 aacaacggat tggctggcag ttggaactgg cagcagagaa agggatcgct ttcggcaata
    51061 tcgacgccat cgtcgagcat attcagcagc gtctggcctc gcatcaaccg cagccttctc
    51121 tgttgcacgg cgatttatgg tccggcaact gtgcgctggg tccggatggc ccgtacattt
    51181 tcgaccctgc ctgctactgg ggtgaccgag agtgcgacct ggcgatgtta ccgctgcata
    51241 ctgaacagcc gccacaaatc tatgacggct atcagtcagt atccccgcta cctgccgatt
    51301 tccttgaacg tcaaccggtt taccaactct acacgctgtt aaatcgtgca agattatttg
    51361 gcggtcagca tttggttatt gctcagcagt cattggatag attattagca gcatgatatg
    51421 ggttgaggat aatggccgct ccgtgcggcc ttttgattaa ataaatccaa gcagagaaaa
    51481 gaacacataa cctgcagcaa tacaaataac cggcaggata tatagtgaga aaaactgcag
    51541 gaaaatagta tggtgcggaa caacaatacg ggactcaatt tgttcacgcg ttagcccctc
    51601 ttcccctttg gctttttcca gaatgagttg atcttcaaca ccttcccgca agaagcgcgc
    51661 ctggcgactc atccgggcac cggaatcctg caacgccagt ccgacaaaaa tcaggatgaa
    51721 aatcacccag aacataacgt tcacgccacc attaaaaata ggggtcgggg agttatacca
    51781 gaacagattc aaaaaaggcg tattcgcctg catcatatcg atcatgacat gagtaaagtc
    51841 gagcatgacc gcattaatgc cttcctggtt ttcctgccgg gtattcataa atttcagcaa
    51901 agaaatcaat gtagagatca atgcaggtat aaaaatcacc caacccaaaa tccttttcaa
    51961 aacagcaatg cgtccagctt gttgatacgt catgagttct ccttgattaa gacgcgtcgt
    52021 ttcacttagt ttacctgtag atatctgttt tcgcccattc tttaaaggcg atatgatagg
    52081 cgcttaatca taagcacggc ttaatacctt acacataatg ctctaaagga gaggttgtaa
    52141 tgtcaacccc gcgtcagatt cttgctgcaa tttttgatgt ggatggatta cttatcgact
    52201 cagaaccttt atgggatcga gccgaactgg atgtgatggc aagcctgggg gtggatatct
    52261 cccgtcgtaa cgagctgccg gacaccttag gtttacgcat cgatatggtg gtcgatcttt
    52321 ggtacgcccg gcaaccgtgg aatgggccaa gccgtcagga agtagtagaa cgggttattg
    52381 cccgtgccat ttcactggtt gaagagacac gtccattatt accaggcgtg cgcgaagctg
    52441 ttgcgttatg caaagaacaa ggtttattgg tgggactggc ctccgcgtca ccactacata
    52501 tgctggaaaa agtgttgacc atgtttgact tacgcgacag tttcgatgcc ctcgcctcgg
    52561 ctgaaaaact gccttacagc aagccgcatc cgcaagtata tctcgactgc gcagcaaaac
    52621 tgggcgttga cccactgacc tgcgtggcgc tggaagactc ggtaaatggc atgatcgctt
    52681 ctaaagcagc ccgcatgcgt tccatcgtcg ttcctgcgcc agaagcgcaa aatgatccac
    52741 gttttgtatt agcaaacgtc aaactctcat cgctgacaga actcaccgca aaagaccttc
    52801 tcggttaatg accaggggca gtgatcacgc tgcccctggt tctttatctg aatttgcatt
    52861 caactgacgg attaatcgtc aatttaagag aaagagttac accgtcacca cttccgtgca
    52921 ctgtataaaa atcctatact gtacgtatcg acagtttagt gagttttatc atgacggcgg
    52981 aaggtcacct tctcttttct attgcttgtg cggtatttgc caaaaatgcc gagctgacgc
    53041 ccgtgctggc acagggtgac tggtggcata ttgtcccttc cgcaatcctg acgtgtttgt
    53101 taccggacat cgatcaccca aagtcgtttc ttgggcagcg attaaaatgg atatcaaaac
    53161 cgatcgcccg cgcttttggg catcgtggtt ttacccacag tctgctggcg gtatttgcgc
    53221 tgctggcaac cttttacctt aaggttccgg aaggctggtt cattccggct gatgcgctac
    53281 aaggaatggt gctgggttat ttgagccaca tacttgccga tatgctgaca cccgccggtg
    53341 ttcccctgct ctggccatgc cgctggcgtt tccgcttgcc tatcctggtt ccccaaaagg
    53401 gcaaccaact ggaacgtttt atctgcatgg cattatttgt ctggtcggta tggatgcccc
    53461 attcattacc cgagaacagc gctgttcgtt ggtcatcgca aatgatcaat accttgcaga
    53521 tccagtttca tcggcttatt aagcatcagg ttgaatacta aaaagacaaa aatcaccttt
    53581 ctggaataag caattccatt tgaatataag agccagctca cagttctgtt aatcttgcgc
    53641 caacactatg actgctacgc agtgatagaa ataataagat caggagaacg gggatgaact
    53701 ttccattaat tgcgaacatc gtggtgttcg ttgtactgct gtttgcgctg gctcagaccc
    53761 gccataaaca gtggagtctg gcgaaaaaag tgctggtggg tctggtgatg ggtgtggttt
    53821 ttggccttgc cctgcatacc atttatggtt ctgacagcca ggtacttaaa gattctgtac
    53881 agtggtttaa catcgttggt aacggctatg ttcaactgct gcaaatgatc gttatgccgt
    53941 tagtcttcgc ctctattctg agcgcggttg cccgtctgca taacgcatct cagttaggca
    54001 aaatcagttt tctgaccatc ggtacgcttt tgtttaccac gctgattgcg gcgctggtcg
    54061 gtgtgctggt caccaacctg tttggtttga cggctgaagg tctggttcag ggtggtgcag
    54121 aaactgcacg tctgaacgcc attgaaagta actatgttgg taaagtctct gatctgagcg
    54181 ttccgcagct ggtcttgtcc tttatcccga aaaacccgtt tgccgatctt accggagcca
    54241 atccgacgtc aattatcagc gtggtaattt ttgccgcatt cctcggcgta gctgcattga
    54301 aactgctgaa ggatgatgcg ccgaaaggtg aacgcgtctt agccgctatc gataccctac
    54361 aaagctgggt gatgaaactg gttcgccttg tcatgcagtt gaccccttac ggcgttctgg
    54421 ctctaatgac caaagtggtt gcaggttcta acttgcaaga catcatcaaa ctgggaagtt
    54481 tcgttgtcgc gtcctacctc ggtctgctga ttatgtttgc agtgcatggc attctgctgg
    54541 gcattaatgg cgtgagtccg ctgaagtact tccgtaaggt atggcctgtg ctgacgtttg
    54601 cctttaccag ccgttccagt gctgcgtcta tcccactgaa tgtggaagca caaacgcgtc
    54661 gtctgggcgt tcctgaatcc atcgccagtt tcgccgcctc tttcggtgca accattggtc
    54721 agaacggctg cgccggtttg tatccggcaa tgctggcggt gatggttgcg cctacggttg
    54781 gcattaaccc gctggacccg atgtggattg cgacgctggt cggtattgtt accgttagtt
    54841 ccgcaggcgt tgccggtgtc ggtggtggtg caactttcgc cgcactgatt gtgctgcctg
    54901 cgatgggcct gccagtaacc ctggtggcgc tgttaatctc cgttgaaccg cttatcgaca
    54961 tgggccgtac ggcgctaaac gttagtggct cgatgacagc tggcacgctg accagccagt
    55021 ggctgaagca aaccgataaa gccattctgg atagcgaaga cgacgccgaa ctggcacacc
    55081 gttaattctt atgctggcaa cggtccgttt tgtataggag ccgttgcttt actttttaaa
    55141 tgttccatcg cggcgatgca tactgactca tatttaaaca gatcgtcagc gaaaaaaatg
    55201 tccaacttat caatacattc ctggctcaat gaatgtgcct gtagtgagaa gcttaaaacc
    55261 tcatacccga gacgttttct aagtgaacta tctggaatac tcataatatc ttcaagcaca
    55321 ccacttccca ttcccggaag atttagtgga ttaaaaagaa gattgatatt aagcattgat
    55381 attatcgaag gaaactttag gagactctca ttttttttga atcgcagcaa aaatgctccc
    55441 ggcaggatgt atataatttt tttcatagta tatagtcctt aaataatgaa taaccaacct
    55501 attttttata ttgatcaaat tcgtcatttt ttcgggtcgg aattcacagg taaaatccat
    55561 cccaacaaaa ttctcattaa tatctcttat ccgaaactca ctgccataaa gattattaat
    55621 tatcactcta tatttaacat tgtcctgcag atacgtatca aaactaagtg ctggcgctcc
    55681 tggctttgag gcatcatagt taatgttaga catataggca atcatacgat tgaagttcga
    55741 ctctgctatt tcatcgcagt cacattgacc cgttgatata aagaaagtga gtgccgccat
    55801 ggtagactca agcctttctt taaaagagtc gaaagccagg cttttaatat ttaaatcacc
    55861 ataattactc tgtattaagt ttgtagaaaa catctcccgc cccatattgt taacaaaatt
    55921 attatctcat ttaaatctaa g
//
LOCUS       DAZKHC020000020        53310 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZKHC020000020 DAZKHC020000000
VERSION     DAZKHC020000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 53310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.020"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            52..1818
                     /gene="adeD"
                     /locus_tag="ACHOSM_RS07230"
                     /old_locus_tag="pgaptmp_001446"
     CDS             52..1818
                     /gene="adeD"
                     /locus_tag="ACHOSM_RS07230"
                     /old_locus_tag="pgaptmp_001446"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HGU4558000.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1863..3254)
                     /gene="uhpT"
                     /locus_tag="ACHOSM_RS07235"
                     /old_locus_tag="pgaptmp_001447"
     CDS             complement(1863..3254)
                     /gene="uhpT"
                     /locus_tag="ACHOSM_RS07235"
                     /old_locus_tag="pgaptmp_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HGU4558001.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3392..4711)
                     /gene="uhpC"
                     /locus_tag="ACHOSM_RS07240"
                     /old_locus_tag="pgaptmp_001448"
     CDS             complement(3392..4711)
                     /gene="uhpC"
                     /locus_tag="ACHOSM_RS07240"
                     /old_locus_tag="pgaptmp_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGU4558002.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGVSALLLLPFLNAQAPREA"
     gene            complement(4721..6223)
                     /gene="uhpB"
                     /locus_tag="ACHOSM_RS07245"
                     /old_locus_tag="pgaptmp_001449"
     CDS             complement(4721..6223)
                     /gene="uhpB"
                     /locus_tag="ACHOSM_RS07245"
                     /old_locus_tag="pgaptmp_001449"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HGU4558003.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6223..6813)
                     /gene="uhpA"
                     /locus_tag="ACHOSM_RS07250"
                     /old_locus_tag="pgaptmp_001450"
     CDS             complement(6223..6813)
                     /gene="uhpA"
                     /locus_tag="ACHOSM_RS07250"
                     /old_locus_tag="pgaptmp_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HGU4558004.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6888..7178)
                     /gene="ilvN"
                     /locus_tag="ACHOSM_RS07255"
                     /old_locus_tag="pgaptmp_001451"
     CDS             complement(6888..7178)
                     /gene="ilvN"
                     /locus_tag="ACHOSM_RS07255"
                     /old_locus_tag="pgaptmp_001451"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU4558005.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7182..8870)
                     /gene="ilvB"
                     /locus_tag="ACHOSM_RS07260"
                     /old_locus_tag="pgaptmp_001452"
     CDS             complement(7182..8870)
                     /gene="ilvB"
                     /locus_tag="ACHOSM_RS07260"
                     /old_locus_tag="pgaptmp_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGU4558006.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNKALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8976..9074)
                     /gene="ivbL"
                     /locus_tag="ACHOSM_RS07265"
                     /old_locus_tag="pgaptmp_001453"
     CDS             complement(8976..9074)
                     /gene="ivbL"
                     /locus_tag="ACHOSM_RS07265"
                     /old_locus_tag="pgaptmp_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HGU4558007.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            9639..9728
                     /gene="tisB"
                     /locus_tag="ACHOSM_RS07270"
                     /old_locus_tag="pgaptmp_001454"
     CDS             9639..9728
                     /gene="tisB"
                     /locus_tag="ACHOSM_RS07270"
                     /old_locus_tag="pgaptmp_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HGU4558008.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9991..10065)
                     /gene="ysdE"
                     /locus_tag="ACHOSM_RS07275"
                     /old_locus_tag="pgaptmp_001455"
     CDS             complement(9991..10065)
                     /gene="ysdE"
                     /locus_tag="ACHOSM_RS07275"
                     /old_locus_tag="pgaptmp_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HGU4558009.1"
                     /translation="MNVSQIYDRNGELFSGRICKQKRQ"
     gene            10147..11331
                     /gene="emrD"
                     /locus_tag="ACHOSM_RS07280"
                     /old_locus_tag="pgaptmp_001456"
     CDS             10147..11331
                     /gene="emrD"
                     /locus_tag="ACHOSM_RS07280"
                     /old_locus_tag="pgaptmp_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HGU4558010.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNGGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11339..11836)
                     /gene="yidF"
                     /locus_tag="ACHOSM_RS07285"
                     /old_locus_tag="pgaptmp_001457"
     CDS             complement(11339..11836)
                     /gene="yidF"
                     /locus_tag="ACHOSM_RS07285"
                     /old_locus_tag="pgaptmp_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGU4558011.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDSTLQKWCGSENP
                     QSRRDCQACDWHRLCPHAREETPDSMLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11833..12195)
                     /gene="yidG"
                     /locus_tag="ACHOSM_RS07290"
                     /old_locus_tag="pgaptmp_001458"
     CDS             complement(11833..12195)
                     /gene="yidG"
                     /locus_tag="ACHOSM_RS07290"
                     /old_locus_tag="pgaptmp_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HGU4558012.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12185..12532)
                     /gene="yidH"
                     /locus_tag="ACHOSM_RS07295"
                     /old_locus_tag="pgaptmp_001459"
     CDS             complement(12185..12532)
                     /gene="yidH"
                     /locus_tag="ACHOSM_RS07295"
                     /old_locus_tag="pgaptmp_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HGU4558013.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12641..13090
                     /gene="yidI"
                     /locus_tag="ACHOSM_RS07300"
                     /old_locus_tag="pgaptmp_001460"
     CDS             12641..13090
                     /gene="yidI"
                     /locus_tag="ACHOSM_RS07300"
                     /old_locus_tag="pgaptmp_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HGU4558014.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVERKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13137..14630)
                     /gene="yidJ"
                     /locus_tag="ACHOSM_RS07305"
                     /old_locus_tag="pgaptmp_001461"
     CDS             complement(13137..14630)
                     /gene="yidJ"
                     /locus_tag="ACHOSM_RS07305"
                     /old_locus_tag="pgaptmp_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4558015.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTIGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARTEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYQLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVNTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(14627..16342)
                     /gene="yidK"
                     /locus_tag="ACHOSM_RS07310"
                     /old_locus_tag="pgaptmp_001462"
     CDS             complement(14627..16342)
                     /gene="yidK"
                     /locus_tag="ACHOSM_RS07310"
                     /old_locus_tag="pgaptmp_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HGU4558016.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMSVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPHATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWQHRHDPAVTFTPD
                     AKDSL"
     gene            16509..17402
                     /gene="yidL"
                     /locus_tag="ACHOSM_RS07315"
                     /old_locus_tag="pgaptmp_001463"
     CDS             16509..17402
                     /gene="yidL"
                     /locus_tag="ACHOSM_RS07315"
                     /old_locus_tag="pgaptmp_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU4558017.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17399..18213)
                     /locus_tag="ACHOSM_RS07320"
                     /old_locus_tag="pgaptmp_001464"
                     /pseudo
     CDS             complement(17399..18213)
                     /locus_tag="ACHOSM_RS07320"
                     /old_locus_tag="pgaptmp_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18213..19829)
                     /locus_tag="ACHOSM_RS07325"
                     /old_locus_tag="pgaptmp_001465"
     CDS             complement(18213..19829)
                     /locus_tag="ACHOSM_RS07325"
                     /old_locus_tag="pgaptmp_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU4558018.1"
                     /translation="MLSQIQGFGGAMFTPVLLFPFARIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGGGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            20125..20841
                     /gene="yidP"
                     /locus_tag="ACHOSM_RS07330"
                     /old_locus_tag="pgaptmp_001466"
     CDS             20125..20841
                     /gene="yidP"
                     /locus_tag="ACHOSM_RS07330"
                     /old_locus_tag="pgaptmp_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4558019.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(20838..22499)
                     /gene="yidE"
                     /locus_tag="ACHOSM_RS07335"
                     /old_locus_tag="pgaptmp_001467"
     CDS             complement(20838..22499)
                     /gene="yidE"
                     /locus_tag="ACHOSM_RS07335"
                     /old_locus_tag="pgaptmp_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HGU4558020.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQVGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22695..23123)
                     /gene="ibpB"
                     /locus_tag="ACHOSM_RS07340"
                     /old_locus_tag="pgaptmp_001468"
     CDS             complement(22695..23123)
                     /gene="ibpB"
                     /locus_tag="ACHOSM_RS07340"
                     /old_locus_tag="pgaptmp_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HGU4558021.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(23235..23648)
                     /gene="ibpA"
                     /locus_tag="ACHOSM_RS07345"
                     /old_locus_tag="pgaptmp_001469"
     CDS             complement(23235..23648)
                     /gene="ibpA"
                     /locus_tag="ACHOSM_RS07345"
                     /old_locus_tag="pgaptmp_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HGU4558022.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23642..23714)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23954..24286
                     /gene="yidQ"
                     /locus_tag="ACHOSM_RS07350"
                     /old_locus_tag="pgaptmp_001470"
     CDS             23954..24286
                     /gene="yidQ"
                     /locus_tag="ACHOSM_RS07350"
                     /old_locus_tag="pgaptmp_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU4558023.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24288..25502)
                     /gene="yidR"
                     /locus_tag="ACHOSM_RS07355"
                     /old_locus_tag="pgaptmp_001471"
     CDS             complement(24288..25502)
                     /gene="yidR"
                     /locus_tag="ACHOSM_RS07355"
                     /old_locus_tag="pgaptmp_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HGU4558024.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            25603..26667
                     /gene="cbrA"
                     /locus_tag="ACHOSM_RS07360"
                     /old_locus_tag="pgaptmp_001472"
     CDS             25603..26667
                     /gene="cbrA"
                     /locus_tag="ACHOSM_RS07360"
                     /old_locus_tag="pgaptmp_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HGU4558025.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(26664..28001)
                     /gene="dgoT"
                     /locus_tag="ACHOSM_RS07365"
                     /old_locus_tag="pgaptmp_001473"
     CDS             complement(26664..28001)
                     /gene="dgoT"
                     /locus_tag="ACHOSM_RS07365"
                     /old_locus_tag="pgaptmp_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4558026.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(28076..29224)
                     /gene="dgoD"
                     /locus_tag="ACHOSM_RS07370"
                     /old_locus_tag="pgaptmp_001474"
     CDS             complement(28076..29224)
                     /gene="dgoD"
                     /locus_tag="ACHOSM_RS07370"
                     /old_locus_tag="pgaptmp_001474"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HGU4558027.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(29221..29838)
                     /gene="dgoA"
                     /locus_tag="ACHOSM_RS07375"
                     /old_locus_tag="pgaptmp_001475"
     CDS             complement(29221..29838)
                     /gene="dgoA"
                     /locus_tag="ACHOSM_RS07375"
                     /old_locus_tag="pgaptmp_001475"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HGU4558028.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGSGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29822..30700)
                     /gene="dgoK"
                     /locus_tag="ACHOSM_RS07380"
                     /old_locus_tag="pgaptmp_001476"
     CDS             complement(29822..30700)
                     /gene="dgoK"
                     /locus_tag="ACHOSM_RS07380"
                     /old_locus_tag="pgaptmp_001476"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HGU4558029.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30697..31386)
                     /gene="dgoR"
                     /locus_tag="ACHOSM_RS07385"
                     /old_locus_tag="pgaptmp_001477"
     CDS             complement(30697..31386)
                     /gene="dgoR"
                     /locus_tag="ACHOSM_RS07385"
                     /old_locus_tag="pgaptmp_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HGU4558030.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31664..32255
                     /locus_tag="ACHOSM_RS07390"
                     /old_locus_tag="pgaptmp_001478"
                     /pseudo
     CDS             31664..32255
                     /locus_tag="ACHOSM_RS07390"
                     /old_locus_tag="pgaptmp_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32301..33113)
                     /gene="yidA"
                     /locus_tag="ACHOSM_RS07395"
                     /old_locus_tag="pgaptmp_001479"
     CDS             complement(32301..33113)
                     /gene="yidA"
                     /locus_tag="ACHOSM_RS07395"
                     /old_locus_tag="pgaptmp_001479"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGU4558031.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMTI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33228..33626)
                     /gene="yidB"
                     /locus_tag="ACHOSM_RS07400"
                     /old_locus_tag="pgaptmp_001480"
     CDS             complement(33228..33626)
                     /gene="yidB"
                     /locus_tag="ACHOSM_RS07400"
                     /old_locus_tag="pgaptmp_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HGU4558032.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(33866..36280)
                     /gene="gyrB"
                     /locus_tag="ACHOSM_RS07405"
                     /old_locus_tag="pgaptmp_001481"
     CDS             complement(33866..36280)
                     /gene="gyrB"
                     /locus_tag="ACHOSM_RS07405"
                     /old_locus_tag="pgaptmp_001481"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGU4558033.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36309..37382)
                     /gene="recF"
                     /locus_tag="ACHOSM_RS07410"
                     /old_locus_tag="pgaptmp_001482"
     CDS             complement(36309..37382)
                     /gene="recF"
                     /locus_tag="ACHOSM_RS07410"
                     /old_locus_tag="pgaptmp_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGU4558034.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37382..38482)
                     /gene="dnaN"
                     /locus_tag="ACHOSM_RS07415"
                     /old_locus_tag="pgaptmp_001483"
     CDS             complement(37382..38482)
                     /gene="dnaN"
                     /locus_tag="ACHOSM_RS07415"
                     /old_locus_tag="pgaptmp_001483"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGU4558035.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38487..39890)
                     /gene="dnaA"
                     /locus_tag="ACHOSM_RS07420"
                     /old_locus_tag="pgaptmp_001484"
     CDS             complement(38487..39890)
                     /gene="dnaA"
                     /locus_tag="ACHOSM_RS07420"
                     /old_locus_tag="pgaptmp_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGU4558036.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40187..40267
                     /locus_tag="ACHOSM_RS07425"
                     /old_locus_tag="pgaptmp_001485"
     CDS             40187..40267
                     /locus_tag="ACHOSM_RS07425"
                     /old_locus_tag="pgaptmp_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4558037.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40497..40637
                     /gene="rpmH"
                     /locus_tag="ACHOSM_RS07430"
                     /old_locus_tag="pgaptmp_001486"
     CDS             40497..40637
                     /gene="rpmH"
                     /locus_tag="ACHOSM_RS07430"
                     /old_locus_tag="pgaptmp_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGU4558038.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40654..41013
                     /gene="rnpA"
                     /locus_tag="ACHOSM_RS07435"
                     /old_locus_tag="pgaptmp_001487"
     CDS             40654..41013
                     /gene="rnpA"
                     /locus_tag="ACHOSM_RS07435"
                     /old_locus_tag="pgaptmp_001487"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGU4558039.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            40977..41234
                     /gene="yidD"
                     /locus_tag="ACHOSM_RS07440"
                     /old_locus_tag="pgaptmp_001488"
     CDS             40977..41234
                     /gene="yidD"
                     /locus_tag="ACHOSM_RS07440"
                     /old_locus_tag="pgaptmp_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGU4558040.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41237..42883
                     /gene="yidC"
                     /locus_tag="ACHOSM_RS07445"
                     /old_locus_tag="pgaptmp_001489"
     CDS             41237..42883
                     /gene="yidC"
                     /locus_tag="ACHOSM_RS07445"
                     /old_locus_tag="pgaptmp_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGU4558041.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAANQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            42989..44353
                     /gene="mnmE"
                     /locus_tag="ACHOSM_RS07450"
                     /old_locus_tag="pgaptmp_001490"
     CDS             42989..44353
                     /gene="mnmE"
                     /locus_tag="ACHOSM_RS07450"
                     /old_locus_tag="pgaptmp_001490"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGU4558042.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            44596..44670
                     /gene="tnaC"
                     /locus_tag="ACHOSM_RS07455"
                     /old_locus_tag="pgaptmp_001491"
     CDS             44596..44670
                     /gene="tnaC"
                     /locus_tag="ACHOSM_RS07455"
                     /old_locus_tag="pgaptmp_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HGU4558043.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            44891..46306
                     /gene="tnaA"
                     /locus_tag="ACHOSM_RS07460"
                     /old_locus_tag="pgaptmp_001492"
     CDS             44891..46306
                     /gene="tnaA"
                     /locus_tag="ACHOSM_RS07460"
                     /old_locus_tag="pgaptmp_001492"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HGU4558044.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCVKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            46397..47644
                     /gene="tnaB"
                     /locus_tag="ACHOSM_RS07465"
                     /old_locus_tag="pgaptmp_001493"
     CDS             46397..47644
                     /gene="tnaB"
                     /locus_tag="ACHOSM_RS07465"
                     /old_locus_tag="pgaptmp_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HGU4558045.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            47776..48951
                     /gene="mdtL"
                     /locus_tag="ACHOSM_RS07470"
                     /old_locus_tag="pgaptmp_001494"
     CDS             47776..48951
                     /gene="mdtL"
                     /locus_tag="ACHOSM_RS07470"
                     /old_locus_tag="pgaptmp_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HGU4558046.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            48926..49885
                     /gene="yidZ"
                     /locus_tag="ACHOSM_RS07475"
                     /old_locus_tag="pgaptmp_001495"
                     /pseudo
     CDS             48926..49885
                     /gene="yidZ"
                     /locus_tag="ACHOSM_RS07475"
                     /old_locus_tag="pgaptmp_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
     gene            50042..50791
                     /gene="yieE"
                     /locus_tag="ACHOSM_RS07480"
                     /old_locus_tag="pgaptmp_001496"
     CDS             50042..50791
                     /gene="yieE"
                     /locus_tag="ACHOSM_RS07480"
                     /old_locus_tag="pgaptmp_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HGU4558047.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            50813..51379
                     /gene="yieF"
                     /locus_tag="ACHOSM_RS07485"
                     /old_locus_tag="pgaptmp_001497"
                     /gene_synonym="chrR"
     CDS             50813..51379
                     /gene="yieF"
                     /locus_tag="ACHOSM_RS07485"
                     /old_locus_tag="pgaptmp_001497"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HGU4558048.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAKQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(51433..52770)
                     /gene="adeP"
                     /locus_tag="ACHOSM_RS07490"
                     /old_locus_tag="pgaptmp_001498"
     CDS             complement(51433..52770)
                     /gene="adeP"
                     /locus_tag="ACHOSM_RS07490"
                     /old_locus_tag="pgaptmp_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HGU4558049.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            52937..>53310
                     /gene="yieH"
                     /locus_tag="ACHOSM_RS07495"
                     /old_locus_tag="pgaptmp_001499"
     CDS             52937..>53310
                     /gene="yieH"
                     /locus_tag="ACHOSM_RS07495"
                     /old_locus_tag="pgaptmp_001499"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HGU4558050.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNM"
BASE COUNT        12924 a        14265 c        13618 g        12503 t
ORIGIN      
        1 tttctttatg ataaataata atcaaattga taaaatcaaa atgagaaaaa tatgaataat
       61 tccattaacc ataaatttca tcacattagc cgggctgaat accaggaatt gttagccgtt
      121 tcccgtggcg acgctgttgc cgattatatt attgataatg tctctattct cgacctgatc
      181 aatggcggag aaatttccgg cccaattgtg attaaaggac gttacattgc cggtgttggc
      241 gcagaatacg ctgatgctcc ggctttgcag cggattgatg ctcgcggcgc aacggcggtg
      301 ccagggttta ttgatgctca cctgcatatt gaatccagca tgatgacgcc ggtcaccttt
      361 gaaaccgcta ccctgccgcg cggcctgacg accgttattt gcgaccctca tgaaatcgtc
      421 aacgtgatgg gagaagccgg attcgcctgg tttgcccgct gtgccgaaca ggcaaggcaa
      481 aaccagtact tacaggtcag ctcttgcgta cccgccctgg aaggctgcga tgttaacggt
      541 gccagtttta cccttgaaca gatgctcgcc tggcgggacc atccgcaggt taccggcctt
      601 gcagaaatga tggactaccc tggcgtaatt agcgggcaga atgcgctgct cgataaactg
      661 gatgcatttc gccacctgac gctggacggt cactgcccgg gtttgggtgg taaagaactt
      721 aacgcctata ttactgcggg tattgaaaac tgccacgaaa gttatcagct ggaagaagga
      781 cgccggaaat tacaactcgg catgtcgttg atgatccgcg aagggtccgc tgcccgcaat
      841 ctcaacgcgc tggcaccgtt gatcaacgaa tttaacagcc cgcaatgcat gctctgtacc
      901 gatgaccgta acccgtggga gatcgcccat gaaggacaca tcgatgcctt aattcgccgc
      961 ctgatcgaac aacacaatgt gccgctgcat gtggcatatc gcgtcgccag ctggtcgacg
     1021 gcgcgccact ttggtctgaa tcacctcggc ttactggcac ccggcaagca ggccgatatc
     1081 gtcctgttga gcgatgcgcg taaggtcacg gtgcagcagg tactggtgaa aggcgagccg
     1141 attgatgcgc aaaccttaca ggcggaagag tcggcgagac tggcacaatc cgctccgcca
     1201 tatggcaaca ccattgcccg ccagccagtt tccgccagcg actttgccct gcaatttacg
     1261 cccggaaaac gctatcgggt cattgacgtc atccataacg aattgattac gcactcccac
     1321 tccagcgtct acagcgaaaa tggttttgat cgcgatgatg tgtgctttat tgccgtactt
     1381 gagcgttacg ggcaacggct ggctccggct tgtggtttgc ttggcggctt tggactgaat
     1441 gaaggtgcgc tggctgcgac ggtcagccat gacagccata atattgtggt gatcggtcgc
     1501 agtgccgaag agatggcgct ggcggtcaat caggtgattc aggatggcgg cgggctgtgc
     1561 gtggtacgta acggccaggt ccaaagtcat ctgccgttgc ccattgccgg cctgatgagc
     1621 accgacacgg cgcagtcgct ggcggaacaa attgacgcct tgaaagccgc cgcccgtgaa
     1681 tgcggtccgt tacccgatga gccgtttatt cagatggcgt ttctttctct gccagtgatc
     1741 cccgcgctaa aactaaccag tcaggggcta tttgatggcg agaagtttgc cttcactacg
     1801 ctggaagtca cggaataata aaaaagcccg gcgtcatgcc gggcaaaagt caccagttac
     1861 gtttatgcca ctgtcaactg ctgaattttt ttctcgcggc ggattttgcg ttcttccatc
     1921 accgccacta tcgccatcag gcagatacaa ccaatcgcgg cgatatcaag tgcggcgaag
     1981 gtgcctgccc agccggtaag gccgaatacc ggcgtcccat cggcaatcat tcccagacct
     2041 aacttggcaa agctgtcacc aatcaggtaa gcaaaggtgc ctttaatacc atcggcagcg
     2101 ccaatcgctt ttttaggtac aaagccaaca gcagccacac caatcaacaa ttgcgggcca
     2161 aagaccaaga aacccaacgc aaagagagaa gccagataga tatattcgtt gctggcatgt
     2221 tgatacacac cgagcgtggc gataatcagc gccagcgcga tgcaggccac caggccacgg
     2281 cgaccgttcg ccaggtcaga gagccagccc cacagcagcg taccgaccag cgcaccagct
     2341 tcaaacagcg taaagccctg aatcgccacc gctttagaga gtttcagttc ctggaacgcg
     2401 tatacggttg accactggtc gataccaata cgtaccacat agaggaaaat gttggcgaag
     2461 cacagcagcc agatcacttt gtttttcagc acatactcaa caaagatctg ccacttggtc
     2521 atatcggtag attctgtctc tttgtcctct tcgctgatct cctcgccgaa cagttcttca
     2581 gctttgccga ggccataaga ttccggggag tcgctgccgt aacgcaggcc gataaaaccg
     2641 acaatcagcg cgataatcga cgggaagata aacatgccga tgacatggcc atcgaacagg
     2701 taatttgccc cgaacagcgc cacacctgct gcgcctgcac cgccaaggtt gtgagaaata
     2761 ttccagaaac cgaggaatgt cccgcgttta cgacgcggcg tccatttggt gatggtggag
     2821 taactgcacg aaccgccggt actctggaaa aagccgctta aggcgtagaa ggcaatcatc
     2881 aggaacaggc taaccgagcc gctgcccata ctggcactga agcccagcat acaaatggca
     2941 gacaggatca gcatgaacgg caggaattgt ttggtgtttt tgccgtcggc gtagtatgaa
     3001 accaacgttt tacccacgcc ataagtgatg gagaaaccca ggccgatcat ccccagctgc
     3061 gtcatgctca acccgtaggt cgaaatcata tcgttctgcg cgatgttaaa gttcttgcga
     3121 atcaggtaca tcgtcaggta gccgataaag accaccaggt aggattgcat gaacggtttg
     3181 aaccacattt tgcgccgcac ttcgagcgga aggtccaggg tcggcttgcg aacctggttt
     3241 aggaaagcca gcatgggtta ctcctgaaat gaatacctgc gtgaggcatg cattgtaaaa
     3301 atcagcagca aatcaggcct gagatagcgt ccaggcaaaa cctggaaaat ttcttagttt
     3361 tgccggatat gaagtgaaaa ggtgagatgc atcacgcttc gcgcggtgcc tgggcgttca
     3421 aaaagggcag taacagcagt gcggaaaccc cggcggcgat agagataacc acaaaaaatc
     3481 cgctccagtg ccaggtatcg agtactttcg ccagcggcca accagcaagc gacgccccca
     3541 gataagcaaa caagccgaca aaccccgtcg ccgcccctgc agcctctttg tgggaacact
     3601 ctgccgccgc catgccgatt aacatctgcg ggccaaagac aaaaaaacca atggtgaaga
     3661 agcaggttgc ctgcatcacg tagctggcaa atggcatcag ccacagcgag ccgactgaaa
     3721 gcaaaattcc ggcggcgaaa atcaaattca tcggcccacg gttgccgtta aacaatttgt
     3781 ccgagcccca accggctacc agcgcaccga taaatccgcc cagttcaaac atcgtcactg
     3841 ccgtattcgc cgtgaccaga tcgacgccca gcgtctcgga catatacaaa ttgccccagt
     3901 cgttgattgc cgcccggacc acatagacca gcacatagca aaacgaaagc agccagatat
     3961 acggattcag caacacatat ttggtcagga tctctttacg cgtcaaccct gccccttctt
     4021 gttgttgagc aatttccagc gcgtcgtgcc gccattcacc gaccgccggt aaacctaacg
     4081 cctgcgggcg atcgcgtagc cgccagcaga gaaaaatccc cacgactatc gccatacaac
     4141 cagcaatcat catcccggca cgccagccgt aatgcagcgc gctcgctgcc atcacaatgg
     4201 gaatgagtgc gccgccgacg ttatgcgccg tgttccataa tgcccaccaa ccgccgcgct
     4261 cggtacgtga ataccaggcc gttaacagac gcgcacacac cggtgaaccc cagccctgga
     4321 aaaaggcgtt cagcacccag agcacggcaa acgcccatag cgacgtcgag aagccaaaca
     4381 gaatgttcat gatgcccgtg gcgataagcc ctatccccat aaaataacgg gcatttgagc
     4441 gatcgctgac aatgccggag acaaacttcg acacgccgta agtaatgtaa aacagggtcg
     4501 ctaacaggcc gatatcgcta cggctgagca cgccgttagc aaggatttct ggtaccgcgg
     4561 cgttaaaact tttccgcgtg aagtaaaaca gcgcgtaacc cagccagatg gtcagcagaa
     4621 tatgccgacg ccagtagcga taacgggcat caatttcatg tttatcagtc attaatggcg
     4681 catcggcagg cgctttcaga aacggcaaca tcatcgaacc ttagacataa cgttgaggta
     4741 gagaaacgct gacacgcgtg ccgtgcagac aggaaatgtg taatgtgccg cccagcgccg
     4801 ttacgcgctc gcgcattccg gtgaggccaa aaccttgttg cccggaaccc ggcggcaaac
     4861 cgctgccatc gtcttcaatc accagcatca accgttcatc ctgctgccag ccttgcagcg
     4921 taaccgcgct ggcatcggca tgtttcacaa tgttgttcag cccttcctgg cagacacgaa
     4981 acagcgtcac gcgctggttt tcgcttaatg ctgattcatc gatacgccat tcgagatggc
     5041 tgacaatgcc gcgcccttcc agctccattt cccgcatcag tgagcggatg gcctgctcca
     5101 gggtgagatc atccagctgg cgcggacgta accgtcctaa caaacggcgc accgcatcgt
     5161 aaacacccaa cgacagttgt tcgatgagct gcccgctctg cttcacgctg gcgttatctg
     5221 ccgccagccg ctgaacaatg cccgcctgag tacgaatagc agtgatggtc tgaccgatat
     5281 catcatgcag ctcacgcgcc acatcacggc gcacgctctc ttcggtttcc agcaaccgtt
     5341 cagccagatg ctgattgcgc gccagttcct tttgcagcga ctggttaagt tcacgcaacc
     5401 gctggatgcc agcgccaagc aacaaccctg tcagactttg caccagcagc gagagcaata
     5461 aatccaccgg atgatcgcgc caggtttgac tggcgatcag cgcgatggcg ttcatcaacg
     5521 tcgcaatcag cgccccttgc caaccatagt gccaggccag cgcgataatc ggcagcgcca
     5581 gacagaatgg cgtaaagcgc gacagttcgt ccggcaatcc caactggagc cagagactga
     5641 taacaaacag cagcaagtac cagaccagat gtcgcccgcg ccagttgatt ggctgagaaa
     5701 ccagtgacgg gccgagcggc agccaggtgt tattggcgag atagtgccag aacaccagac
     5761 atatcggggc cagcgtcagg ccgccagtta aagtcagcaa cagcgcattc cacgactctt
     5821 tgccgtgcca aagccagggc agcgactgca acaacgccgc cgccgttaac gccgccccct
     5881 gtaacagcaa ggtgcgccag tcacgctgat ggcgatagcg cgagatcagc gctacgggca
     5941 gtaacgtcag taaactaccg atcatcaata acggaaaatg ggttaaaccg accgcctgcg
     6001 ttagccagta aatcagcagc cactccgcgc ccagcagcac cggccagtat ccgcgcgggc
     6061 attgcagcat tagccccaga cgcagaccaa acggaaataa cagcaccgcc atatcagggc
     6121 gctcaaccag atgcaggctg atactccaca ggcaaaacca tgcggcagag aagataaaaa
     6181 agcaggcaat aacggtaatt aagcgggaga acaacgtctt catcaccagc catcaaacat
     6241 gcggcgcgcc agctctacgt cgttactgac gcccagtttt tccatcagat tggcgcgatg
     6301 gacgtgtacc gttttcggtg acaagcccag ttcggcggca atctctttca ccgccattcc
     6361 ttgcgccagt ttttcagcca cctggcgttc acgtttggtt agcgggtcct gacgaccgga
     6421 tgccagttta atggcaatat ccggcgtcag ataacagccg cccgtggcaa ccgtatgcac
     6481 cgcagcaatg agttcatccg ggctacagcg tttggaaaga aagccgcgtg cccccgcgtt
     6541 aagcgcctgc tcaaccagcg caggactgtc gtgaacggag agcataatcg tcgccatacc
     6601 tttcggcagc tggcttagca gctccagacc ggagatatcg ggcatggaga tatcgcaaat
     6661 acacacctgc acaccgcgcc ccggcagccc cgccagcgcc tcgcgacccg aaccaaactc
     6721 ggcaactacc tgcaaatcag gttccagccc cagcagctgc gcaaagccgg agcggacgat
     6781 gaggtgatcg tctataaggg caacggtgat catggtcttg tcctggcggg taaaaaaaaa
     6841 cgcgcttacc ttaacgataa gcgcgatgtt gttcaagcct tgagcggtta ctgaaaaaac
     6901 accgcgatct tgttaaacat cgtcggatcg gactgattac gctgcacttt cacgacatct
     6961 tccagcttat cgatttggct tatcatctgc tccagacgct ggtcgtcatt gaccagtagc
     7021 cagatatggc ttttgtcgct gtcctgaatc ggcagacaaa gaatgccttc aacgttaaaa
     7081 gcgcggcggg caaaaaggcc acaaacgtgg gtcattacgc ccggatggtt gcgaacggtg
     7141 agctccagaa ttacgttgtc atgagttgtg ttttgcatgg cttattcccc caccatttca
     7201 gtattcgccg cacctggcgg caccatcggg taaacttttt cttcggcatc aatgcgcaca
     7261 tggatcagcg ccgggccagg gcgattgatg atttcctgca atgcagcctg cggatcggct
     7321 tcgttattca aatcacaggt ttcgaggccg aatccggcgg caatctgcat aaagttgatt
     7381 ttgcccggat aggtggcggc aaaaacgcct tgctcgtaga acagactctg ttgctgatgc
     7441 accagcccca gcgctttgtt gttcatcaga atgattttga catccagctg attttcactg
     7501 gcggtcgcca tctcctgaat attcatcatc aggctgccgt cgccggagaa acacaacact
     7561 ttgcgatccg ggttcgccag cgccgcgcca atcgccgcag gcaggccaaa acccatcgtg
     7621 cccagcccac cggaggtcag ccactggcgt gggcgattga gcggataagc ttgcgcggtc
     7681 cacatctgat gctgacccac atcggtggtg ataatcgcat tgtcatcgac acaggcggca
     7741 acggcgttga tcaggccgta atggcttaat ggatcgcacg ctttcgggat tggacacggg
     7801 aattcgcgct gcaaatccgc taccaactgg tgccactctg cacgcggttg cgcttccacc
     7861 tgcgggatca attgcgccag cacgtcatca acatccgcct gaatcgccac atgcggctgc
     7921 ttgattttac ccagctctgc gcgatcgata tcgacatgaa taattttggc attcggacag
     7981 aactgctcgg ttttgccaat cgcccggtca tcaaaacgcg caccgagcac tatcaacaaa
     8041 tccgcctcct gcaaaatata gttggtgctg cgcacgccgt gcatccccag catacccagc
     8101 gacaacggat gcgcttttgg caacatgccc agcgccatta aagtcatggt ggtaggcagt
     8161 tgcgctttct ccgccagttc acgcacccgt gcgggcgcat tgatcacacc gccgcccaga
     8221 taaagcaccg ggcgtttggc agcgttaatc atcgccgctg cgtcacgaat gctttcttcg
     8281 ctaaaggcgg gggcggcggc tttttctgcc atagcgggct gtgtttcaat ctcaaaaact
     8341 gccgtttgca catccttagg aatgtctatc cacaccgggc ctgggcggcc tgattgcgca
     8401 atgcggaagg catcgctcat gacctgcggg agttcttcga tatgtctgac cagatagttg
     8461 tgtttggtga tggggataga gatgccgtag gtgtccactt cctggaaggc gtcggtgccg
     8521 atcatcgagg cgggaacctg accggtgatg caaatcagcg ggatggagtc cagccgcgca
     8581 tcggcaatgg cggtcaccag gttagtcgca cccggtccgc tacaggccat acagaccgcc
     8641 ggtttaccgt cggtgcgcgc catcccctga gcgataaatc ccgcgccctg ttcatgacgg
     8701 gctagaatat ggcggatttg cgtgctttgg cttaaggcat cgtaaacagg caggatagaa
     8761 ccgcccggaa tgcccgtcac aatcttaatg ccctgctgtt ccaggaaatg aacgataaat
     8821 tctgcgccgg taaagcgctt acgcgtcgat gttgtgcccg aacttgccat gctccagtcc
     8881 ttttcttctg ggccgacttt ccgggaggtg cttaaacgaa aaaccccgcc cggtttgcgc
     8941 cggcggggtt ttggaatcgt gtgttgttcc agtccctacg gcgcattgcc gacgaccacc
     9001 accacacgca cgacgaccac tgcggcggat agcgcagttg gtagtagttt tgcgttgaac
     9061 atggaagtag tcattgggga ccttgttggt tttgtgttta acaatattta tacaagcaca
     9121 gctttacagg ggagacaatg gaaaattttt cagcaaggga aaattgaggg gctgatcacg
     9181 ttttgtactg aattgcaggt aacaaaaaac cccgccggag cgaggtttca tcagccgcct
     9241 gcggctggta accgcaaagc acactgtatt atgtcaacac tgaaagtata cgtgttccgc
     9301 gcagaacgcg caatttcggc acgaattttg acgtatttag ggcatagttg agtatcgatc
     9361 acagtttgcg ttttgtccaa atattactgt ttatttatac agtaaacttc tataatatca
     9421 ctgtacgcaa tgtgttatgc gggggccgca tcgttacccg gcgcactaag tcctggctga
     9481 aacgggtggt gccgtcagcg ccttaacccc gcgtgagcac actgtgttat gtcaacaagc
     9541 acaacgtttc tccttgagat accgcgtgca caacagctgg caacaggcag cggaaaggta
     9601 cgtcagctgg cagtgctcct gaaccacagg agacgcgtat gagcctggtg gatatcgcca
     9661 ttcttatcct caaactcatt gttgcagcac tgcaactgct tgatgctgtt ctgaaatacc
     9721 tgaagtaatt cagattcaag tcgcaccaaa ggggagcggg taaccgctcc ccttttatat
     9781 ttagcgtgcg ggttggagtt gggggcttct tctaccagca gaatgtttgc acctgcgctg
     9841 cctgtgcccc tcaccctagc cctctcccca aaggggcgag gggaccgtcc cgtgcaatgt
     9901 ttagctcatt ttctgaaagg gaacggtggt ttccccctcg cccctttggg gagagggtta
     9961 cagttcgcac gtaatccctc ccggcaacat ttattgccgc ttttgtttac atattctgcc
    10021 gctaaacaat tccccattcc tgtcgtatat ctggctaaca ttcatcaatg tgatagattc
    10081 ctctcccgca tttatgggaa tgcgtagtga cttattctaa ttatttttat aaaagcatcc
    10141 gtgataatga aaaggcaaag aaacgtcaat ttgttattga tgttggtatt actcgtggcc
    10201 gtcggtcaga tggcgcaaac catttatatt ccagctattg ccgatatggc gcgcgatctc
    10261 aacgtccgtg aaggggcggt gcagagcgta atgggcgctt atctgctgac ttacggtgtc
    10321 tcgcagctgt tttatggccc gatatccgac cgcgtgggtc gccgaccagt gatcctcgtc
    10381 ggaatgtcca tttttatgct ggcaacgctg gtcgcggtca cgacctccag tttgacggta
    10441 ttgattgccg ccagcgcgat gcaggggatg ggcaccggcg ttggcggcgt aatggcgcgt
    10501 actttgccgc gtgatttata tgaacggaca cagttgcgcc atgctaacag cctgttaaac
    10561 atggggattc ttgttagtcc gttgctcgca ccgctaatcg gcggtctgct ggatacgatg
    10621 tggaactggc gcgcctgtta tctctttttg ttggttcttt gtgctggtgt gaccttcagt
    10681 atggcccgct ggatgccgga aacgcgtccg gtcgatgcac cgcgcacgcg cctgcttacc
    10741 agttataaaa cgcttttcgg taacggcggt tttaactgtt atttgctgat gctgattggc
    10801 ggtctggccg gaattgccgc ctttgaagcc tgctccggcg tgctgatggg cgcggtgtta
    10861 gggctgagca gtatgacggt cagtattttg tttattctgc cgattccggc ggcatttttt
    10921 ggggcatggt ttgccgggcg tcctaataaa cgcttctcaa cgttgatgtg gcagtcggtt
    10981 atctgctgcc tgctggctgg cttgctgatg tggatccccg actggtttgg cgtgatgaat
    11041 gtctggacgc tgcttgttcc cgccgcgctg ttctttttcg gtgccgggat gctgtttccg
    11101 ctggcgacca gcggcgcgat ggagccgttc cccttcctgg cgggcacggc tggcgcgctg
    11161 gtcggcggtc tgcaaaacat tggttccggc gtgctggcgt cgctctcagc gatgttgccg
    11221 caaaccggtc agggcagcct ggggttgttg atgaccttaa tgggattgtt gatcgtgctg
    11281 tgctggctac cgctggcaac gcggatgtcg catcaggggc agcccgttta agttcacgtc
    11341 accgcagcat cgtcatcagc tccatgggag aacgatgctg ctttatcaga tcacgcatca
    11401 cccgcatatg cggtgcggag taagaataaa acgcctgata gcccgcacaa agcatgctgt
    11461 ctggcgtctc ctcccgagcg tgcggacaca gccgatgcca gtcgcacgcc tggcagtcac
    11521 ggcgcgattg cgggttttcg ctgccacacc atttttgtag ggtggagtca aaaagctcaa
    11581 tcgagatacg actgatatct gcccgcaccc aacactcgaa cagggcaatt aaaaatttcc
    11641 cccacagctc tgctgtcacc gaagcggccg tcatatcgcc ccggtcatca aattcaagat
    11701 ggggaatgag ctgtacggag cggatgcccc gttgtttgaa attgtgataa aaatcggcag
    11761 gttgcagagc atccttgtac tctacaacca gtttatgcct gtcctcttca gcatcaatga
    11821 cctgacttcc tgtcatgcga ctctctcaat aaatacgata agttgatgta tatgcgttac
    11881 agcaaacagt attgcgagag ataacaccgc gagggagatc aaaaatttgt cacggactac
    11941 gtgaaattgg gaaaaatcgc tattcgtgac atccattaaa ttgcgattac gggtgtagtg
    12001 ccagaggatc agcgccacga tggcgaggat gccaatcgaa atccagaata acatgcccgc
    12061 ctggtgccag ttgtgtttga tagccaacgc catcaacgcg ccgtagccca gcatggtacg
    12121 aaaccacgcc agtgatgtac gctccggctg cagccctggg tcggcaatgc gtctggcttt
    12181 gcggctatcc ggcatacaac accagtccca taacgatcac tgctacgacc atcaatatta
    12241 agctgatgat taataagctg ttggtgtagg gcaggtcctc tttcagacgc atcgcttttt
    12301 cattgcgtaa ccagcgcaag tagccataca tcgctaaccc gccggaaaac agacacaaca
    12361 acaacgccag cagttcgcga atgacgggcg tggcgaaatc tggcgcaagt tgatcaagcc
    12421 caacccccgc cgccagaaat cctagtgcag tacggatcca cgccagaaaa gtacgctcat
    12481 tagccagcga gaagcggtaa tcaggcgctt ctccgaggcg ggaaattttc attgttgctc
    12541 ctttgcaatt tcactaacgc tctaccttta gcggtttttt ttaagaattt cgtccgcccg
    12601 tttcctgata tgcttttgcc atccgctaca aggaggtcag atgggtatca tagcgcaaaa
    12661 taaaatcagt tcattgggca tgctattcgg tgccatcgct ttaatgatgg ggattatcca
    12721 cttttcattc ggcccttttt ccgcaccacc gccaactctt gaaagcatcg tggcggacaa
    12781 aacggctgaa attaaaagag gtcttctcgc cggaataaaa ggcgaaaaaa ttaccaccgt
    12841 ggagagaaaa gaggacgtgg atgtcgataa aatcctcgat caaagcggta tcgcactggc
    12901 cattgctgcg ctactttgcg catttattgg cggtatgcgt aaggaaaatc gctggggcat
    12961 tcgcggcgcg ctggtttttg gcggcgggac gctggctttc cataccttgc tttttggtat
    13021 cggcatcgtg tgcagcattt tgctgatatt ccttatcttt tccttcctga ctggcggttc
    13081 actggtttaa gcagcctgaa taatgcccgc cgggtgttca tcagcgggca ttatcgtcag
    13141 aacttctgtt ttttctcttc caccttcacc ccttgtgtcg gcaggccggt gtcatagtcg
    13201 cgtaccactg gcgaataacc atcttgtggg cgtggacgaa atgcccccat ccagcgcggc
    13261 tgtgcatctt tacgccacgg acgcaggctc cattggtaac tgcggaatgg atcgcgaatt
    13321 ttgtccatgt aatccaataa ggcgtcatgc attttgctgc gcacgtcggc aaaacggata
    13381 tcatctatca ggttatgcat ttcatttgga tcattacggc gatcgtaaag ttcatcactg
    13441 gtgaagaggt tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa
    13501 ccgccaaagc tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc
    13561 tctttcacgg caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc
    13621 gccatcattg tcggcagtaa atcgatatga ctgactggcg tattgacctg tcgccgctcc
    13681 ccttgcggcg aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct
    13741 ttactgatca gcttatgtgc gcccatcatt tcgccgtgat cggatgtata aataacccac
    13801 gtattttcac gttgctctgg cgttaaggca ttgatgaccc gtccgatttg gtcatcaaca
    13861 aagtcattac aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat
    13921 ggcatcgcct gcgcccataa gcggtgatgt tctggtttgt tcgccaggtc atcctgtgct
    13981 ttctcgccca gttggtagta aaaatcagcg tatttctcta aatactccac cggacaggtg
    14041 aacgggtgat gcggctcatc atacgaaacc accatcagga agggttcctc ggtgcgcgcg
    14101 ggctgttgca gaaaatccac cgcccgattg ctgatgcggt gcgcccaggt gaaggtttcg
    14161 tcgatatggt tcgcctgtaa atcctcaacg ctgtttaggc cattgcgcca caggctaatc
    14221 tctttctccg tcagttcgct aagatagttc gccccatcga accagtaatc agcatcccac
    14281 tccggcggac actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg
    14341 taacaggtgt gatagccggc atccttaaag tagcgcccaa tagtggagat gtttttgcct
    14401 ggcgcgacgt tgttggtcca cgggccggac tggttagcgt agataccggt aaacagcccg
    14461 gcgcgcgccg gtgtacaaac cggtgaacag gtgtaggcgg agttaaagcg aataccttcc
    14521 gccgccagac tatcaatatt ttgcgtattc agcggcttac cgctatagca accgaccata
    14581 ttggtggcct gggtatcggt catgatgaac agaaaattgg ggcgtttcat aggctatcct
    14641 tcgcgtcggg agtaaaggtt acggctgggt cgtgacgatg ctgccagctg tcaaaaatca
    14701 ggtagacaat cactacggcg gcaatgaaat aactgatcat cgctaaccag cgcgtaccgt
    14761 agccgccgaa ttcagccagc ccggcataga cgccaatcat cgcgaacagg atgccaattg
    14821 acgcgatctt gacgtttttc cacggtttca tgtccaccgc aaacgcatct ttgaaggtga
    14881 acggcgtggc gtgcggtttg ataaaaccga tcaccagcat cacgaccacg ttgatgcaga
    14941 acgtacaggc cagcacatag aggaaatgga agtcgaactt caccagatag ttgatggtga
    15001 tgtagctgat tatgccaatc cccatcgcca cttttgccgc cagcgccggg atgcgcggga
    15061 agaaaaagcc cataatgatg atggtaacca gcggcacgtt gtaaatgccg ttgagctgtt
    15121 tcatccagct atacagcccc tgcggcgcgt tggcgatcca cggcgctacc agcaccgaaa
    15181 cgatagcgat aaagaaacca aatttgcgcc cgacggtgac cagctgctgc ggctcggcat
    15241 tctggttaat gatgcgacgg taaataccca tactgaataa cgtactggcg ctattcagaa
    15301 agccgttgaa ggtactgatc accgcaccaa ataacaccgc gccgaagaaa cccaccagtg
    15361 gcactggcag aacgttattg accagcgtcg ggtaggccat gtcggctttc ggtagatcct
    15421 gatacagatg aaacgcaatc aaccctggca gcaccagtac cagcgggtca agcattttca
    15481 gcaccgccgt taacagcgcc cccttttgcc cttccgccag gctttttgac gccagcgtgc
    15541 gttgcacgat gccctgattt gtacaccagt aaaaggtgtt caccagaatt aaaccggtaa
    15601 atgccgcgcc aatcggcaag ggatcggtcg ggccaccgac tgagtttaat ttctcggcgt
    15661 gaacggtggt aatttgctca atgccctgca taaagctgcc cttgcccatc gcgatcaggc
    15721 caaataccgg caccatcaac ccgccgataa ccagcccaat accgttgatg gagtccgcca
    15781 ctgacattgc gcgcagtccg ccgatcaccg catacaaaat tcccgccaga ccaagcaaaa
    15841 ttaccagtag ccagatagcc gcaccgtgag aaatctgtag cgattccccg acgtgaaaca
    15901 ggctgttgag cgccaacgcg ccggagtaga gaacaatcgg cagaaagcag acgccggtgg
    15961 caatgaggaa gcagaagtcg atgataatac gcgtcgtttt atcataacgt tcctccagaa
    16021 aatcggggat ggtggcaatg ccgcgcttca gatagcgcgg taaaaagatt agcgcgagga
    16081 agatcagcgt caccgctgaa gtcacttccc agcccatcac cgacatgccg cttttgtagg
    16141 cctgcccgga aagaccaacc agttgttccg tggaaaggtt ggttaacatt aacgaagcgg
    16201 caataaccgg cgcttttagt gaacgtccgg caagaaaata gccttgttgc gatccggtat
    16261 ctgttttgcg aaccttccac caggtgatga tcgccaccag cagcgtaaaa ccgacaaaac
    16321 tcaagatttg taacgaattc atctcatagc ccttatttat gtttatgtcc cggacgcatg
    16381 aatataggga gaggtaaaat gcgtcgggtc aggcagtcag attcttaaaa tacaaacgtc
    16441 gtatccctga acggattgtt ttctgttaag ttcaggttgt gagcataatt cgccggaggg
    16501 atattaaaat gaatggaaaa ttgcaaagtt cagatgtaaa aaacgaaact ccgtacaata
    16561 ttcccttatt gattaacgaa aatgtgatct ccagcggaat ttctctgatc tcgctctggc
    16621 atacctacgc cgacgagcat taccgggtga tctggccgcg ggacaagaag aaaccgctta
    16681 tcgccaactc atgggttgcg gtgtataccg tacaaggatg tgggaaaatt cttttaaaga
    16741 atggcgaaca aataacactg catggcaact gtattatatt tttaaagcca atggatattc
    16801 actcttatca ctgtgaaggt ttagtctggg aacagtactg gatggaattt acccccacca
    16861 gtatgatgga tattcccgtt ggtcagcaaa gcgttattta taatggcgaa atttataatc
    16921 aggaactcac cgaagttgct gagttaataa cttcaccaga agcaataaaa aataatctgg
    16981 cagtcgcttt tctgacgaaa attatttatc agtggatttg tcttatgtac gcagatggta
    17041 aaaaagatcc acaacggcgg caaattgaaa aattaattgc cactttacat gccagtctgc
    17101 aacaacgctg gagcgtagct gatatggctg ccacgatccc ctgtagcgaa gcctggttgc
    17161 gtcgtctgtt tttacgctat accggcaaga cgccgaaaga atattacctc gatgcgcgtc
    17221 tggatctggc gctatcgcta ttaaagcaac aaggaaactc ggttggcgaa gtcgctgata
    17281 cgctcaactt cttcgactcc tttcatttca gcaaagcctt taaacataaa tttggttatg
    17341 cgccgtcagc cgtgctaaag aatacggacc agcacccaac ggatgccagt ccacacaatt
    17401 aacgtaactc tggccagtac gctttgttgg cctccagcaa ctcatccaga atagctttgg
    17461 cgaccgaggc gctcggtacc gttttcgaca acgtaatcgc ctgccacagg tgctgatatg
    17521 aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc cacttgctga ctcattaacc
    17581 ctttctgaaa ctgcgggata tcgccgacca ccagtggttc aggccgtagt agcgcacgcg
    17641 catctgtttg cgatcttgca gcccaacaat ttgcgccatc cggctttcaa taccgattgg
    17701 catgtcacag atgttgagga ttttcgcatt cgggcgcaga cgacgcgtgg cttctgcgac
    17761 aatggctgcc gggttggagt agttgagcat ccaggcattt ggtgaatatt tttccatata
    17821 atccaccagt tccaggacgc cgccaatgga acgcatgccg tacgctattc cgccgggtcc
    17881 gcaagtttcc tgaccaacaa caccgtggcg cagtgggatt ttttcatcca gttcgcgcat
    17941 cgggtacttg ccgacgcgga tgtgcgccat gacaaaatca acgtcgctga acgccacttc
    18001 aggatcggtg gtgtaactaa acgcaatgtc cggcgctttc tctttaagga tgactttaca
    18061 ggcttcggca atcacttcct gccgcgcgcc atcgttatca taaaatttca gtgcacgaag
    18121 cgggaaacgc tcctgattcg ccaggagcat caacacgatg cctggcgtaa aggtacttcc
    18181 accgcctgcg acaaccactg agaatttggt cattatactg cctccgtaat ggcaacattt
    18241 tctgctgatt gatgagaatt aattaagcta tcgagctgtt cacgcagctg ggatacatgc
    18301 agaccaatga tcacctgaat ggcatcgcca ctacggaaga cgccgtgcgc tcccagcttt
    18361 ttaaagactt cgtcatccag cgtttgtgac atgtcatgca gtgcaatgcg tagacgcgtc
    18421 gcacaattgt taatgctgga gatattgccg cccccgccca gggcttgcag gataccggca
    18481 gcctgatcca gctctttttt tggctctgcc gcggtggttt ggcctcgcga ggctttgtat
    18541 tcggcttttg agtagagttt cacttccgca tcttcacgtc ccggcgtgca catgttgaac
    18601 tgcagaatca gggtgcggaa aaccacgaag tacagcaggg taaagcacaa cccaatggcg
    18661 atttgggtca gcatcatatc cgcgtggttg ctgaacatcg ggatccagtt ttgcggtaaa
    18721 acctggtcaa tcagacctcc gcccatgttg cccaccacac caaagagata catcacggtc
    18781 gacattgagg ccgcaagcac agcgtgtacc gcaaacagca acggtgaaat gaacaggaag
    18841 gtaaattcca gcggttcggt aattcccacc agcatggcgg ttaaggttgc gggaatcagc
    18901 aagcccgcca cttttacccg attttccggt gcggcagtga agtacatcgc taaagaaatg
    18961 cccacggcac caaagatttt tgagttaccg tgcagggcaa aaccgccttc cgggaacaac
    19021 gatttcagcg gctcggcgct cagactgaac tcttgcagat gctgcgccca gtacatctga
    19081 atgccgcctt caacagctgc cggaccaaag ataaactgtc cgtagatgaa gtggtgtaaa
    19141 ccggttggga tcagaatacg ttcgaggaag gtgtaaaccc acaccccaag cgcacccgcc
    19201 gaacgcagga acgcttgcag agattcaatc cccatttgta cttttggcca gccgagcaac
    19261 gtcagccagg cacaggggat catcaccaag aaggcgataa tcaccacata agacgtcccc
    19321 tggaaaatgc cgagaaaaac cggcagtttt ttatcgaaca gacggttatg cagcgccgtc
    19381 acaatgccgg aaatgataat tgcgccgata atgctggtat cgagggtttt aatcccggcc
    19441 atcattgtca gaccgctacc tgccaccgcg tcctgagtga aatcgacgcc gaagtagctt
    19501 ccccaggtca ttcccatcgc gttgatgaaa tagttccagg tcaggaaact caccatcacc
    19561 gccagacaag cacgcccctg cgcttgctta gcaaggccaa tgggtaaacc gacagcaaaa
    19621 atcagcggca tattacggaa taccgtccaa ccgccctctt caataatgtg tacgatttgc
    19681 gcgaataaac tgttcggatc ggtcagtgat tccccgacaa acatcgggtt ttgcagcaag
    19741 atggcaagac ccaccacaat cctggcgaag ggaaacagca gcactggcgt gaacatcgcg
    19801 ccgccaaagc cttgaatttg actgagcatt tccacatcct cttttctcaa ttctgtaagt
    19861 aggaagcctg attggttatg cggcaggcct ggagcgagga taggaagatg cgatcggcag
    19921 aacatgacga cccaatggga ttcgtgatcg ctttcatgct tttcgttggg cggtagagat
    19981 ctacgttttc gcgaaaaagt agacatctgt gagcactggt acacagcatc gaacacatct
    20041 ttaaaaaaaa gatgtttttt caatcgatta agcagaactt gtgggcgcat tacccgggct
    20101 tgcaggcaaa aaagagatct agagatgatc tacaaaagca ttgcggagcg gttaagaatt
    20161 cgacttaatt ccgcggattt cacgctaaac agccttcttc ccggtgaaaa aaagttggcg
    20221 gaagaatttg cagtatcccg gatgaccatc cgtaaagcga ttgacctgct ggtagcgtgg
    20281 gggctggtgg tccgccgcca cggcagcggc acttacctgg tgcgcaaaga tgtgctgcat
    20341 caaaccgcca gcctgaccgg actggtggag gtgttaaaac ggcagggaaa aacggtcacc
    20401 agccaggtgc tgatttttga aatcatgcct gcgcctccgg ccattgccag ccagttacgg
    20461 attcaaatca acgagcagat ctacttctcc cgtcgcgttc gttttgtgga agggaaaccg
    20521 ctgatgctgg aagacagcta tatgccggta aaactgttcc gtaatctttc gctgcaacat
    20581 ctggaagggt cgaagtttga atatattgaa caagagtgcg ggattttgat tggcggtaat
    20641 tatgaaagcc tgacgccagt gctcgccgat agactgctgg cgcggcaaat gaaggtagcg
    20701 gaacacacgc cactgctgcg gatcacctcg ttgtcatata gcgagagcgg ggagtttttg
    20761 aattattcag tgatgttcag aaatgccagc gaataccagg tggagtacca tttacggcga
    20821 ctccacccgg aaaagagtta accgatactc cagaagagca ccgccagtaa ttggggggtg
    20881 ataatgcgca ggaacattac caacggatag acagtggcgt aagagagcgc cgccgcaccg
    20941 ctggttggat gaagattatt agcaaacgcc agcgccggag gatcggtcat ggaacctgcc
    21001 agcatcccgc acatggtcag gtaattcatt ttggctaaca tccgcgccag aatgccaaca
    21061 gtaatcagcg gaacggcggt gatcagggca ccataaccaa tccagcttag cccttcgcca
    21121 ttgaccaggg tattcacaaa atctccacca gatttcagac caacgaccga gaggaacagc
    21181 acgatcccca gctcccgcag cgcgaggttg gcacttggcg gcataaacca gtacagcttg
    21241 ccgatactgc cgatacgccc gaggatcaac gccataatca gcggaccgcc cgccagcccc
    21301 agtttcaacg cggccgggaa tcccggcaca aagacgggaa tagaacctaa caatacgcca
    21361 agcccgatgc caataaacac tggcagcatc tgaacctgtt gcagtttttg ctgcgcattc
    21421 cccagcacat tggcaacggc atcaattgcg gacggacgcc ccaccagatt gaggatatcg
    21481 ccgaactgca ggctgatatc gccactggcg accagttcga ccccggcacg gttcaggcgc
    21541 gagataacaa cgtcatagcg ttctttaaaa tgcaggtcgc gaatacgctt accaagcacg
    21601 ttttcattgg tgaccaccac acgctcgacg cgcaaatcag tgcctttcgt ggacagcgag
    21661 gtatcgacct cctgaccaat caccagttgc gcattatgta aatccgctgg ctgccccacc
    21721 agatgcagca aatcgcccag ttggataatg gtatctggcg aaggtacttt tagggtttct
    21781 tctcgtttca gacgcgagca gataattttg tcgccgttga gaatcggtac atctttaatg
    21841 gctaaatcat gcaggttagg gttctcaacg cgaatattga tagtcttgat cagcgcgccg
    21901 ccattggtgc gtgaagactc gtgctgctga gcttctgtct cgacattgac gcggaaaata
    21961 acccgcaaca tccacatggt gaacaaaatc ccgcaaatgc cgaatggata cgccatcgcg
    22021 taactcatcc ccacctgatc gaccatttcc attggtgtac ccaggtcgcg caaaatctgc
    22081 tgccctgccc ccagcgctgg cgtattggta accgcaccgg agaaaatccc cagcactacc
    22141 ggcagtggaa tatcaaacag tttatgcagg atggcggtaa ccagaccacc gatgatgacg
    22201 atcagaacag caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc aaagaagccc
    22261 ggccctacct gaatcccgat ggtataaacg aacaggatca ggccaaattc ctgaataaca
    22321 tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc gacgatgatc
    22381 ccaccaaaca gcacgccgcc aatacctaat cctatgccgc gaaatttgac gttgccgata
    22441 aacaaaccga cgactgccac caaagccaga atactgaccg ttaatgctat atcactcatc
    22501 gtccatttct cttgagataa cattttaatt gctacggatt atggctgaat cggtgaagca
    22561 gatttgcctt atagcgcaca ttatcacggg aaagaacgtg ccgaaatatc ttaaacagtc
    22621 gaaatttaac gcattgttga agaagaaaga caaaaaaagc cccaccagaa tggcggggca
    22681 aagagaatag ctagttagct atttaacgcg ggacgttcgc tgatagcgat acgctgcgct
    22741 gcgatgggtt caggctcatt acgaattaaa tcaatatgca gtaaaccgtt tacgaaggtt
    22801 gcgccagaga cttccatatt ttcagccagc gtaaagctca ggctaaatgg ctgattcata
    22861 agcccttgat gcagccattt tttctcttct tttggctgct ccggcgtgcc ttttacgctc
    22921 aggcgcgtac cttccagttg aatctctaaa tcttcctgac ggaaacctgc cagcgcaagg
    22981 gtaatgcggt agtggttatc gtcgcttttc tcaatgttgt acggcgggaa gctctgactt
    23041 tcaccggcgt tttgcagtgc gttggccagt ttgtcaaaac cgatccattg acgcatcagt
    23101 ggggataaat cgaagttacg catagtcatt tctccttcta agaagcgagt aagtacctgc
    23161 aaatccgaag attcgcatat gctccctgac ggcgagcatg gagatgtcag gccgcgccag
    23221 gcggccttaa ggaattagtt gatttcgata cggcgcggtt ttttcgcttc cggaatcacg
    23281 cgttcgagat cgatatacag caaaccattt accaggttag cgccacgaac atgaatgttc
    23341 tcagctaact ggaatttgcg ttcaaagttg cgttcagcga tgccctggta cagataggtg
    23401 cgctcttttt gttcgtcggc gtgagcacct ttcaccacca gcagattatc ctgggcggta
    23461 atttccagtt cgctctcagc aaaaccagcc acagcgatag caatgcggta atggttttcg
    23521 tctaccagtt caacgttata cggagggtag ccgccattac tctggctctg gttgttttct
    23581 aagtggttaa acaaacggtc aaatccaata gcagaacggt aaagcgggga taaatcaaag
    23641 ttacgcataa tcaatagctc ctgaaatcag cgagaatgta agaccttcca caatggacag
    23701 gtcaggtagc cagaacaccc atcaggcgcg tcctcatcgg ctacgatgta aaaatgggtc
    23761 tggaaatgaa cttttcaagc cttatcagcg gacttttttt tgcagtttat ggtctattgc
    23821 atagactgag ggggcagcac gaaacgttaa agtgcgatag ccgccacagt gcgacgtaat
    23881 gatgatgtta ttttcagcaa gggatcgcta taactcatca ggccaacacc cacgatcaac
    23941 agatgaattt attatgataa gaaatgtgtt gttagcgttc atgatatgca gcggaatgac
    24001 attactcgga ggatgctcca gcgtgatgtc ccataccggg gggaaagaag gaacgtatcc
    24061 cggcacccgc gccagcgcca ccatgattgg cgacgatgaa accaactggg gcactaagtc
    24121 gctggcgatt ctcgatatgc cgtttaccgc tgtgatggac acgcttttac tgccgtggga
    24181 tgtgttccgc aaagacagct cggtgcgctc gcgcgttgaa aaaagcgaag ccaacgcgca
    24241 agccaccaat gcggttattc caccggccag aatgcctgat aactgattta gcgatcagtt
    24301 tcggtgatcc acagctggcc gccttccatc cacgccagcc actgaccatc cggcgagaag
    24361 accacggcgt ccgcagaagg tggattggcg tggttttcgg ttaaatactc aacctcgccg
    24421 ctttgcgcat gggcacaagc aattcgatta tccagcacaa agcccaacca ttctcctgac
    24481 ggatgccagt taaatgcaga ctgaatatcc gttttgttat gggttaactg gcgcggctcg
    24541 ccgccctgtg gcgagataag ccacagttgc acaatgccgt tatcatcacg cattaaaaac
    24601 gcgatttgcg taccctgcgg attacagcgc acccagtggc gcgggacgtt gactaacccc
    24661 ggataagccc gatggtgggt aaaggttaaa cgtcgctgca cgacgccgcg cggtggcgcg
    24721 ggcagggtgg tttccgttcc acttaacggc gcatcacctg ccgctttcca gccagcttca
    24781 tcttgcggta actcaacgat aaacagctcc ggcactttct cgccctttgg cgaaagtgtg
    24841 tcgccaataa acgccagcgc gtgatttcct acccatcctt cttcataagc acgattgatt
    24901 tcatcgctgc caggctgcgg cgtgggcgtg gttttactca ccagcacgca ccagtggcta
    24961 ccgctgtatt cacgcggatg ctgcttttgt acgttgaccg ggccaaacgg cgcagcaaca
    25021 ccgacgtttc gcaaatccag cgccggatcg agttcatgca ttacatggtc gttataggta
    25081 aagctcaccc tttcaccgtt cgggctaaag acatgcacat ggctgccgcc gcgcagcgct
    25141 cctggcgtat acggcgcggt aatatccatt gcatcgagat tgctcacctt gccgccttca
    25201 gcaatgactc cgcgtcgatg atggaaatcg taatgccatg tttcatcagg attttccggg
    25261 ccatggatga aaacatattt ctctgacttc ggatgaacgg tcaccacgcc gacgtgtgcg
    25321 ccctgtgacg cgcgatagat aacctcgacc tcgccggtat ggatattcac acgctcaatg
    25381 gtctcgccgg taaacgacgc cccagaagga cgcacgtcaa ataccagcca ctggctgtcg
    25441 ggcgtccagg tattggtatt ggtgagcagg tgattacggg gagcaaaggt tatctgtttc
    25501 atggcgcggt cctgatacaa aacggggccc gccatcatac ttcacaagga cttcactttc
    25561 agacaatagt cttttgccat cgtgatgaat gtgagtgaaa agatggaaca tttcgacgtg
    25621 gcgattattg gcctcggccc ggcaggatcg gcgttggcgc gaaaattagc aggcaaaatg
    25681 caggtgatcg cgctggataa aaagcaccag tgtggtactg aaggtttcag caaaccttgc
    25741 ggcggtctgc tggcaccgga cgcgcagcga tcttttattc gcgatggact gacgcttcct
    25801 gtcgatgtga tcgccaatcc acagattttc agcgtcaaaa ctgtcgacgt cgccgcatcg
    25861 ctcacgcgta actaccagcg aagctatatc aatattaatc gccatgcttt cgacttgtgg
    25921 atgaaatcgc tgatccccac cagcgttgag gtttatcacg atagcctgtg ccgaaaaatc
    25981 tggcgtgagg atgataaatg gcatgtcatt tttcgtgcag acggctggga gcaacatatt
    26041 accgcccgct atctggtcgg tgccgatggc gcaaactcga tggtgcggcg acatctctac
    26101 ccggatcatc aaatccgtaa atatgtcgct atccagcagt ggttcgcgga gaaacatccg
    26161 gtgccgttct actcctgcat ctttgataat tcgataacta actgttattc atggagtatc
    26221 agcaaagacg gttattttat ctttggcggt gcttatccaa tgaaagacgg tcagacgcgt
    26281 ttcacgacgc tgaaagagaa aatgagcgcc tttcagttcc agtttggtaa ggcggtgaaa
    26341 agcgaaaaat gcacggtgct gtttccctca cgctggcagg attttgtctg cggtaaggac
    26401 aacgcctttc tgattggtga agcggcggga tttatcagcg ccagctcgct ggaggggatt
    26461 agctatgcgc tggatagcgc agacattctg cgttcggtgt tactgaagca gccagagaag
    26521 ctcaataccg cttactggcg cgccacccgc aaactgcgtt taaaactctt cggcaagata
    26581 gtaaaaagcc gatgcctgac cgcaccggct ttaagaaagt ggattatgcg cagtggtgtg
    26641 gcacatattc cacagttgaa ggattagcca acgcgcttca catcgcccac cagcaggata
    26701 taagagagcg cgccaatcaa cgcgacggcg gagatataaa ccagtgcagg tgcgaaaccg
    26761 taaccctgcg ccaggtaccc caccaccagc ggaacggtga tgccgcccag accaccggcg
    26821 aagttaaaca cgccgccggt taaaccaatc aggcgcatcg gtgccagaga agaaaccagc
    26881 gaccaggtaa tcgaagcaaa accgttaccg aagaatgcca gcgccatcag gcacataatc
    26941 atcatcggat cgttggtgta gttagcgccc ataatgcagg tggagatcag caagccgcag
    27001 ataatcggcg ttttacgcgc aaagcccagt gaaaagccct tacgtaccag cagatccgcg
    27061 acccagccag agagcaggac gccgacaaac gccgcgagga atggcaccgt ggtcataaag
    27121 cctgctttca gcgccgtgat tcctttttcc tgggttaaat agttcgggaa ccaggttaag
    27181 aaaaaccaca gtgtagaagc caccgcaaat tgcccaagat aaacgccgat cagtttacga
    27241 tggaacacca gtttccagtc tttggctgtt aacggctgac gcgcctcttt cttcaccggc
    27301 gcatcaccat ccaccagacc gccgccatca cgaatgtaat ccagttcagc tttgctgata
    27361 cctttggtca ggcgcggcgg ctgataaacc ttaaaccaaa tcagcgacca gataatgccg
    27421 ataccaccag tgacaatgaa cacccagtgc cagctcaaca tctcctgaat ccagatcagc
    27481 agcggagtca aaaacgccag accgacaaac tgaccagacg tataaaaacc aacggcggaa
    27541 gcgcgttcat gttccgggaa ccagctggtc accatccggt tattggtcgg gaaagcgggc
    27601 gcttcgaaaa taccggttat cgcacgcaga ccaattaatg acattaatcc cgtggcaaag
    27661 ccctggaata aagtcgccac tgaccaacca aatatcgcaa taaaataagt cacgcgagaa
    27721 cctacgcgat ctaaaaacca accgccgggg atctggcata gcgtataaag ccaggcgaag
    27781 gccgaaaata catagcccat ttccgctttc gtaatgccga actcttcctg aatatgggcg
    27841 gaagccacgg ccaggttagc gcggtcaaca taacaaatga ccaccgtaat aaagatcatc
    27901 accagcgtca gataacgccg acgccccggc tttgctgcat taacgggaat atccatagcg
    27961 agctttctcc agattttggg catagcgaag ccgctcacca tgccctgtaa tttacagagg
    28021 gttatatttt tgtattgctg ttttagtgcc cgatgagggg cttacgtggc aggaattacc
    28081 actctgctac gctgttatct tcatgacgcc agagcggatt acgccagtcc ggggcatttt
    28141 tactgaactc aataacttta gcttcgtcga tttccacgcc taagcccggt ttcgttagcg
    28201 gtttaaagaa gccgccaacc atgctgaagt cttctttgtt tttcacaaag tcgagtaact
    28261 ccgcgccttt gttgtaatga attcccatac tttgttcctg aagtaccgcg ttataggaaa
    28321 caaagtcgat atgcagacaa gccgccagtg caatcggtcc gagcggacag tgcggcgcaa
    28381 gggtcacgtc ataggcttct gccattccgg caattttgta gcattcggta ataccgcctg
    28441 catgggagag atccggttgt agaatcgaaa taccgcctgc ctccagcacg cgtttaaaat
    28501 cgaaacgtga gaacatgcgc tcacccgccg ccagtggaat atgcgtttgt gccgccaatt
    28561 tcgggtagta ttcggcttgt tccgccagca ccggctcttc aataaacagc gggcgatacg
    28621 gctccagttc tttaatcagc actttcgcca ttggcgcgct gacgcgaccg tggaaatcaa
    28681 gaccaaactc aatctgattg ccaaaagctt cacgaatttg tgccacggtg ttgactgccg
    28741 catctaccgc gcgggagtta tcaattagcc ccagttcttc acaaccgttc agtttgaagg
    28801 tatcgaagcc gatttcgcgc agggttttaa taccgtcgat cacatccgcc ggacgatcgc
    28861 cgccgaccca actgtaggct ttaattttgt cgcgaaccag gccgcccatc agttgccaga
    28921 ccggcgcatt cagcacttta cctttgatat cccataacgc ctggtcgatc ccggcaatgg
    28981 cgctcatcag gattggaccg ccacgataaa atccggcgcg atacatcact tgccataagt
    29041 cattgatgcg cgaaggatcc tgaccaatca aatagtcacc cagctcgtga actgccgctt
    29101 ccaccgtacg ggcgcggcct tcaatcacgg gctcgcccca accgaccacg ccttcatcgg
    29161 tttcaatttt caggaacatc cagcggggag gtaaacgata cgtggtaatt ttggtgattt
    29221 tcattgcact gcctctcgat acgccttaac aaatgctgct gcctgctgcg cggtgcgttc
    29281 tacggactgc ccggcgcgat agagatcgct gcctaagccc gccccagcac aacctgcgtc
    29341 tatccactgc gccaggtttt ctggcgtcac gccgccaacg gcaaagactg cgatgtccga
    29401 tggcaatacc gcttttaacg ctttgatgta ttgcggacca aaagccgatg acggaaatat
    29461 tttcagcgcc tgcgcgcccg cttcgagcgc ggtaaaggct tcggtcgccg tcgcgcagcc
    29521 ggggcagacg gtcatgccgt agcccaccgc acggcggatc acttcactat ggatattggg
    29581 cgtaacgatg agctgacagc ccatcctggc gagcgcatcg acctgttcag gtttcagtac
    29641 cgtacctgag ccaatcaacg ccttgtcgcc atacgcatca acgatggcgg gaatgctttg
    29701 ctcccattgt ggggaattca gcgggatttc aaccgcgtcg aacccggcgt caatcaccgc
    29761 gccaacatgc gccagcgcct cgtcgggctt aatgccgcgc aaaatggcga tcagcggaag
    29821 tttagtttgc cactgcatga gcgatgctcc ttataccagc ctgaaatgcc gtgtcgcccg
    29881 ccaccgccgt cacgtcgcaa cccatcgcct gaaaggcttg ctggtagcgc gcggtcagcg
    29941 atgttccggc gacaagggtg atggcgtgtt gatgggtcac atagtcgcgc atactggcaa
    30001 cctctgcgcc aatcaacaaa ccagagagaa attcgctgac ctgttcgcgg ggaagtgttc
    30061 ccagcacatg cgaggcgcga acttcaaaaa gctgcggcaa tatggcgggc gcattaaggc
    30121 cgcgctcaag gccagccgcg aaggcatcgg cagagttttc ctgcggcggc aaacctgcgc
    30181 caatcaatga gtgatttaac agtaaatgat gtagttcacc ggtcatcacg gtgcgaaaat
    30241 cgttgatttg ctggctatcg gcctgcaccc atttgcaatg cgtaccgggc ataacataaa
    30301 gagaggaagg agccagagct cgcgcgccga tcaattgtgt ttcttcgccg cgcatcacat
    30361 tgtggttatc gtcatgagag acgcataatc cgggaataat ccagatattg tcgccaacag
    30421 acgttaattg ttcgccaata gacgaaaaac gggcaggaac agataaatac ggagcaactt
    30481 tccagccgac attgctgccg accattcctg ccattaccac tggcgtattc tcttcacgcc
    30541 agtcggtcgt gacttctgct aacaccgcag ccggagattt tccgttcagg cgcgtgacgc
    30601 ctgcttctga ttgcctgctc tccaggcagt ggtcgccctg ataaagccag gcgcgcagat
    30661 tggtcgatcc ccagtcaatt gcgatgtagc gagctgtcat gtgatttcct ttaaccttcg
    30721 tgtcgagctg gcgatcatgg taagcgccgc ctgctctgcc gcatcgccgt cctgatgccg
    30781 tatcgcatcg aacagcgcct tatgttcctg gagcgtttgc ggcatgttgg cctcatcgcc
    30841 catccaggtt cgttcaaaaa ccgcccgctg cagcgaactg atcgcaatgc taagttgctg
    30901 taacaccggg ttatgcaccg actgcagcac cgcctcgtgg tagcgaatat ccgcttcgtt
    30961 aaacgcttcg cggtcctgat tgttggcaat catctcgttc agcgccgatt caatctgcgc
    31021 cagatcgctg gaggtcgcgc gctctgctgc ccaacgggca atcgccggtt ccaccagatt
    31081 tcgcacttcg ctcatggcac tgataagccg tgggtcgtag tcgttttcca gcacccattg
    31141 cagtacgtca gtgtcgaggt aattccactg gttacgcggt gccacaaacg cgccgcgata
    31201 acgtttcatt tcaatcagcc gtttcgccat caacgaacgg aacacctcac ggatgatgtt
    31261 gcgcgaggtt gcaaactcct cacagagttc cgcctcagcc ggaagcggcg agcctggtac
    31321 gtatttgccg tgaacgatct gtttacccag cgtaatgacg atgcgatcgg ttttattgag
    31381 agtcatggag agtccttgtg ctctgtatgt tcttctctac tttaccccga tcgatgcata
    31441 acacggcaac tttgtagtac cagcgtgatg acgttcgcgt ttgccgtgcg tgtaatgtag
    31501 tacaaactta tattgttgta ctacaattta gatcacaaaa agaacaatgc ataaaaaaat
    31561 gacatgcgtc gggcagaaat ctgaaaaggg atatcaggcg ctaaacagga gggaaagaag
    31621 agtatgcttt caacggctta gctactcgtt taaaggatta atcatgaagt tgaattttaa
    31681 gggatttttt aaggctgccg gtttattccc actggcgctg atgctttcag gctgtatctc
    31741 gtatgctctg gtttcccata ccgcaaaggg tagttcagga aagtatcaat cgcagtcaga
    31801 caccatcact gggctatcgc aggcaaaaga tagtaatgga acaaaaggct atgtttttgt
    31861 aggggaatcg ctggattacc ttatcactga tggtgccgat gacatcgtta agatgctcaa
    31921 tgatccagca cttaaccggc acaatatcgc tttactacta ctggaataac gaagaagaaa
    31981 aggcactggc aacgcattat ggttttgcct gtggtgttca acactgtacc aggtcactgg
    32041 aaaacctaaa aggcacaatc catgagaaaa ataaaaacat ggattactca aaggtgatgg
    32101 cgttctatca tccgtttaaa gtgcgatttt atgaatacta ttcacccaga ggcattccgg
    32161 atggtgtttc cgcagcatta ctgccagtga ctgttacgct ggacatcatt actgcaccgc
    32221 tgcaatttct ggttgtatat gcagtaaacc aataatcagt aagcgggcaa acgcgtttat
    32281 gctgtttgcc cgtccactga ttaattcagc acatacttct caatagcaaa cgccacgcca
    32341 tcttcaaggt tagatttggt gacaaagttc gccacttctt tcaccgaagg aatagcgtta
    32401 tccatcgcca caccgacgcc tgcatattca atcattgcga tatcgttttc ctgatcgcca
    32461 atcgtcatga tttcttccgg tttaatacct aacacgtcgg ccagtgattt cacccccgta
    32521 cctttgttaa cgcgtttatc gaggatttcg aggaagtacg gcgcactttt cagcacggta
    32581 tatttctctt tcacttcctg cggaatacgc gcgatagcct ggtcgaggat ggcgggttca
    32641 tcaatcatca tcactttcag gaactgggta ttggggtcca ttttctccgc ttcgcagaac
    32701 accagcggaa tggtggcaac gaaggattca tgcaccgtgt agtagctgat atcacggttg
    32761 gcggtgtaca gcgtggtgcg gtccagggcg tggaaatgag aaccgacttc gcgagagagt
    32821 ttttccagga aacgatagtc gtcatagctg agagcagttt gcgccacggt gctaccatca
    32881 gcggccttct gtactagcgc gccgttataa gtaatgcagt agtcgcccgg ctgttccata
    32941 tgcagctctt tcaggtagtt gtgcacacct gcatacgggc gacccgtcgt tagcacgaca
    33001 ttcacgccac gggcgcgagc tgcggcaatc gcatttttaa cggcgggtga aatggtgtga
    33061 tcgggcagca gaagggtgcc atccatatcg atagcaatga gtttgatagc catgagttcc
    33121 ccaggtagat tggttcctga cccatgctaa cgcgattccg ctcaaaaatc agtacaacac
    33181 ccgagggaaa agggggatgc aacgcgggtt gcatgctccc catttgctta gcggaagagt
    33241 ttccctttca gcagttccat acctgcggaa agcagatcgt tattggcttg tggtgacact
    33301 tcaccttgcg gtgagagcgc atcaataatc ttcggcaatt gctctgccag taaactggaa
    33361 gctgtactgg tatccacgcc aagtttttgc ccgagatcgg acaccgcatt tgtgccgagc
    33421 gccgattcca gttgctcgcc actaaccgat tgattgccct gttgattact cagccaggtt
    33481 gagagaatgg cccctaagcc gccactttgc agtttttcca gcagcacctg aatgccgccc
    33541 tgctcctcaa cccaacttaa aatagcctga tatttccccg catcgccttt cagaaaggca
    33601 ccgacaactt catcaaaaag ccccatgata atcacctgta aagcgttacg tgttgaccca
    33661 aaaagtatag atttgcggga tgataattgc ggattgcaga aataaaaagg gcggagatga
    33721 tctccgccct tttcttatag cttcttgccg gatgcggcgt gaacgcctta tccggcctac
    33781 aaaatcatga aaattcaata cattgcaaga ttttcgtagg cctgataagc gtagcgcatc
    33841 aggcacgctc gcatggttag cgccattaaa tatcgatatt cgccgctttc agggcgttct
    33901 cttcaataaa cgcacggcgc ggttcaacgg cgtcgcccat cagcgtggtg aacaactggt
    33961 cggcagcaat cgcatcttta acggtaacgc gcagcatacg acggctttcc gggtccatgg
    34021 tggtttccca cagctgttcc gggttcatct cgcccagacc tttataacgc tggatggaga
    34081 ggccgcgacg ggactctttc accagccagt ccagcgcctg ctcgaagctg gctaccggct
    34141 gacgacgctc gccacgttcg ataaacgcat cttcttccag caagccacgc agtttctcac
    34201 ccagcgtgca gatacgacga tattcgccac cggtgataaa ctcgtgatcc agcggatagt
    34261 cagtatccac accgtgggta cgcacgcgaa caatcggttc aaacaggttt tgttcggcat
    34321 tggtgtggac atcaaacttc cactggctgc cgtgctgttc tttgtcgttc agttcgctga
    34381 ccagcgcgtt cacccagcgg gtaacggtct gctcatcaga gaggtcggct tccgtcagcg
    34441 tcggctgata gataagctct ttcagcattg ctttcggata acgacgctcc atgcggttga
    34501 tcattttctg cgtcgcgttg tactcagaca ccaatttctc taacgcttcg ccagccaatg
    34561 ccggtgcgct ggcgttggtg tgcagcgttg cgccatccag cgcgatagag atctggtact
    34621 gatccatcgc ttcgtcgtct ttaatgtact gttcctgctt gcctttcttc actttgtaca
    34681 gcggcggctg agcgatgtag acgtgaccgc gttcaacgat ttccggcatc tgacgataga
    34741 agaaggtcaa cagcagcgta cgaatgtgcg agccgtcgac gtccgcatcg gtcatgatga
    34801 tgatgctgtg ataacgcagt ttgtccgggt tgtactcgtc acgaccgata ccacagccaa
    34861 gcgcggtgat aagcgtcgcc acttcctgag aagagagcat cttatcgaag cgcgctttct
    34921 caacgttgag gattttaccc ttcagcggca gaatcgcctg gttcttgcgg ttacgcccct
    34981 gcttcgcaga gccgcccgcg gagtcccctt ccaccaggta cagttcggaa agcgccggat
    35041 cgcgttcctg gcagtctgcc agtttgcccg gcaggcccgc taagtcgagc gcacctttac
    35101 ggcgggtcat ttcacgcgcg cgacgcgccg cttcacgggc acgggcagca tcgataattt
    35161 tgccaaccac gattttcgcg tcggttgggt tttccagcag gtattctgcc agcagttcgt
    35221 tcatctgctg ttcaaccgct gatttcacct cagaagaaac cagtttgtct ttggtctggg
    35281 aggagaattt cggatccggc actttcacgg aaacgaccgc aatcaggcct tcacgcgcat
    35341 cgtcaccggt ggcgctaact ttggcttttt tgctgtagcc ttctttgtcc atgtaggcgt
    35401 tcagggtacg ggtcatcgcc gcacggaagc ctgccaggtg agtaccgccg tcacgctgcg
    35461 gaatgttgtt ggtaaagcag tagatatttt cctggaagcc atcgttccac tgcaacgcca
    35521 cttcgacgcc aataccgtct ttttcagtgg agaagtagaa gatattcggg tggatcggcg
    35581 ttttgttctt gttcagatat tcaacgaacg ccttgatgcc gccttcatag tggaagtggt
    35641 cttctttgcc gtcgcgcttg tcgcgcagac gaatggaaac gccggagttg aggaacgaca
    35701 actcacgcag acgtttcgcc agaatttcat attcgaactc ggtcacattg gtgaaggttt
    35761 cgaggctggg ccagaaacgc accatggtgc cggttttttc agtctcgccg gtaaccgcca
    35821 gcggagcctg cggtacaccg tgttcgtaga tctgacggtg aattttaccc tcgcgctgga
    35881 taaccagctc cagtttttgc gacagggcgt ttactaccga aacaccaacg ccgtgcagac
    35941 cgccggacac tttataggag ttatcgtcaa atttaccgcc tgcgtgcaga acggtcatga
    36001 tcacttccgc cgccgatacg ccctcttccg ggtgaatacc ggtcggaatg ccgcgcccgt
    36061 catcctgtac agagacagag ttatcggcgt gaatggtgac gataatttct ttacagtgac
    36121 ccgcgagcgc ttcgtcgata gcgttatcta ccacctcgaa taccatgtgg tgcagaccgg
    36181 tgccgtcatc cgtgtcgccg atatacatac ccgggcgctt acgcaccgca tccagccctt
    36241 tcaggacttt gatactggag gagtcataag aattcgacat caacgtttct cgctcattta
    36301 tacttgggtt aatccgttat tttacccttt tccacggtaa acatcttcga attttcgtcc
    36361 gacatgtcta taacgtgttc agcactgatc gcgctgacaa agacctgtga ttgcgtcgct
    36421 tttaagcggc tggcaagcag cccgcgacgc tcatcatcaa gctcagaggc aaaatcatct
    36481 atcaggtaga gacaccgccg cccgctttca cgggtgagga actctccttg cgccagacgt
    36541 aaggcgcaca tcaacagctt aagctgccca cgcgataagg tatcttccac cggcgcaccg
    36601 tcggcgcgaa tgcgtaagtc cgctttatgc gggccatgcg cggtgtaggt tagctggcga
    36661 tcgcgttcaa aattacgttc cagcacctca gcatattctg tctctttctc ccagccgcgc
    36721 tggaaagaga aagtcagaga aaactcaggg agaaattgct tacaggtatc agccatatcg
    36781 gccgcgatac cggcgctata ctccgcgcgc caggtgctga tttgctccgc cagcgggatc
    36841 agctctttat cccacgggcg tagctgttcg taacgtgtca cctggcgcag cgccgcattg
    36901 cgctgcttga gcaatcgctt gagattgctc caggcggtga aaaatccggg ttcgttgtga
    36961 aagcatcccc agtcgaggaa tgctcttctg tatttggggc cgccgttgag taaagtaaac
    37021 ccttctggcg ttatcaactg cattggcatc aggtgcgcca gttccgcgac cttatgcccg
    37081 tctgtaccgt cgatgcggac tttgctgtcg ccctgtttgt ctttggttaa gccaatcgct
    37141 gtctcgcgct cttcgccctg taatcgcccg tggagaacaa acgcctcctg ctcatggcga
    37201 atgacgcgac caatctgcaa actgcgaaac gcccgaccat ggccgagcgt atagatggct
    37261 tccagcacgc tggttttgcc actgccgttg gcacctacca gaaagttaaa gccgggagat
    37321 aaagcgagat ccgcggtttc aatgttgcgg aaatcgcgga tcaacaagcg ggtgagggac
    37381 attacagtct cattggcatg acaacatagg cagccgactg tgatgcggca tcttcaatct
    37441 gcacgctgga aaccgaatcg gtcagcatca tgcggacgtt ttcacatttc agcgcgttca
    37501 gaacatccag cacgtagctg acgttgaagc cgatttccat ctccgcaccg ctataggtaa
    37561 cgtcgaggat ctcttccgct tcttcctgtt ccgggttgtt ggcggtgatt ttcagctggt
    37621 tttcgctgac ataaagacgt acgccgcgga atttctcgtt agagagaatc gccgcgcgag
    37681 caaacgcctg cttgagcaga tcgcagccag cttccagatg tttgtccggg ttcttcggca
    37741 gaacgcggcg ataatccggg aagcgaccat ccaccagttt ggaggtgaag ataaagtcgc
    37801 caacgtgggc gcgaatgttg ttgctgccaa tctgtacgcg cagcggattg tcgccgccgt
    37861 cgagcatacg catcagttca atcacgcctt tacgcggtac gatcaccgaa tggcttggca
    37921 aagattgacc aattggcatt gaacagaccg ccagacggtg gccgtcggtt gccacggtgc
    37981 gcagttcttc accttcggtt tcaaacagca taccatttaa gtaatagcga acgtcctgat
    38041 gcgccataga aaactgggtc gcttcaatca gacgcttcat cgttgcctgc ggcagggtaa
    38101 attcgacttc actctgccag tcatcgaggt tcgggaaatc cgccgctggc agggtagaca
    38161 gcgaaaaacg gctacgcccg gagcgtacca gcatccgttc accttccagc tgcacggcaa
    38221 tttccgcgcc ttcaggcaga ccacggcaga tatcaaagaa tttgcgcgcc ggaacggtcg
    38281 tcgctcctgg ctcgtgtggc tgaaccagcg caacacgtgc caccatttcc atctcgagat
    38341 cagtaccggt cagcgacaac gtaccgtcag caacctgtaa cagcagatta ccgagaatcg
    38401 gtagcgtagg acgaccacct aacggaccgc tcacctgttg tagcggtttt aataaatgct
    38461 cacgttctac ggtaaatttc ataggtttac gatgacaatg ttctgattaa atttgaaaaa
    38521 tcttctttga tatcgtggct ctcttcacgc aactgctcga tcttacggca ggcatgaagc
    38581 accgtcgtgt ggtcacgacc accaaacgca tcgccaatct ccggcagact gtggttagtc
    38641 agctctttcg ccagcgccat cgccatctgg cgcggacgcg ccaccgagcg ggatcgacgc
    38701 ttggaaagga gatccgcgac tttgatcttg tagtactccg ccaccgtctt ctgaatattg
    38761 tcgatggtga ccagtttttc ctgcaatgcc agcaagtcgc gcagcgcctc acgcacgaag
    38821 tcgatggtga tcgcccgtcc ggtaaagttg gcattggcaa tgacgcggtt cagcgcccct
    38881 tccagctcac gtacgttaga tcgtagacgc ttggcgataa agaacgccac ttcgcccggc
    38941 aaacgaatgt cgttttcgtc ggcctttttc atcaggatcg ccacacgggt ttccagctct
    39001 ggcggttcga tcgccacagt cagtccccaa ccgaagcggg atttcaaacg atcctcaacg
    39061 ccgttgatct ctttcggata gcgatccgag gtgagaatga tctgttgatt accttccagc
    39121 agggcgttga aggtatggaa aaactcttcc tgagatcgtt ctttattagc aaaaaactga
    39181 atatcgtcga tcagcagtgc atctacggaa cggtagtagc gtttaaactc ttcgatcgcg
    39241 ttgttttgca gggctttaac catgtcctga acaaagcgct cggagtgcat ataaaccact
    39301 ttggcattcg gcttgcgcgc cataatgccg ttacccaccg catgcagcag gtgagtttta
    39361 cccagacccg tgccgccata aaggaacaat gggttatagg caccgccagg gttatccgcc
    39421 acctggcgag ccgccgcgcg cgccagttgg ttagatttac cttcaacgaa gttatcaaac
    39481 gtgtgtttga cgtttacgtt agaacgatag gtcggttctg ccggggccgg gacgttatcc
    39541 caacctgagc gcgtagaagg cgcagcacgt tgcggctgcg tttgcgccac ctgtgcaggg
    39601 gccgcgacgt tgctcgtcac tgccgcttgt ggcgtttgcg tcaccggttt ggtgccgact
    39661 tcaaaacgca gctgtggggc atccgctccg cagaaactgg ttagcagtcc attgatatta
    39721 ttaaggtact tgtcccgtac ccaatcgagg acaaaacggt ttggcgcgta cagggccagc
    39781 gtgttatcgc tcagttccgc ctgcaatggg cgtatccaca tactgaattc tgtggctggt
    39841 aactcatcct gcaatcgggc aagacactgc tgccaaagcg aaagtgacac ggcggactcc
    39901 actcgaacaa aagtcgataa tgactaaggc tgaaacattc atgattgttg acgtacgtcg
    39961 aaaagaccct gcttgagggt gacgcacgaa ccgctgtctg cggttatatg cccgatcaag
    40021 atcctgcaaa acgatcggga ccgcggatca tagcctaaac tgcgcaagag atcttctgtt
    40081 tctcacagat tttcccgatt tatccacagg actttccaga actcgctaag tgtaaacgat
    40141 cctgccgcga ggcgggcacg atttacgccg catattggaa aatttaatga ccatagacaa
    40201 aaattggctt aatcgatcta ataaagatcc aggacgatcc ttgcgcttta cccatcagcc
    40261 cgtataatcc tccacccggc gcgccatgct ggttttcact ggtgtgaggt cgtacatttt
    40321 ccctgcgaaa aggtgcggaa aagcgcggta aataaggaaa gagaattgac tccggagtgt
    40381 acaattatta caatccggcc tctttaatca cccatggctt cggtgtccat cgtttcattt
    40441 ttcggcggat atccaataaa gccattgaat ttattcaagt ttaggtagaa atcgccatga
    40501 aacgcacttt tcaaccgtct gtactgaagc gcaaccgttc tcacggcttc cgtgctcgta
    40561 tggctactaa aaatggtcgt caggttctgg cacgtcgtcg tgctaaaggc cgcgctcgtc
    40621 tgaccgtttc taagtaataa agctaacccc tgagtggtta agctcgcatt tcccagggag
    40681 ttacgcttgt taactcccag tcaattcaca ttcgtcttcc agcagccaca acgggctggc
    40741 acgccgcaaa ttaccattct cggccgcctg aattcgctgg ggcatccccg tatcggtctt
    40801 acagtcgcca agaaaaacgt tcgacgcgcc catgaacgca atcggattaa acgtctgacg
    40861 cgtgaaagct tccgtctgcg ccaacatgaa ctcccggcta tggatttcgt ggtggtggcg
    40921 aaaaaagggg ttgccgacct cgataaccgt gctctctcgg aagcgttgga aaaattatgg
    40981 cgccgccact gtcgcctggc tcgcgggtcc tgatagccct cattcgggtc tatcaacgcc
    41041 tgattagtcc gctactcggg ccgcattgtc gtttcactcc aacctgttca agctacggaa
    41101 ttgaggcatt gcgcaggttt ggagtgataa aaggcagttg gttgacggtg aaacgcgtat
    41161 taaaatgcca ccctttacac cctggtggtg acgatcccgt cccgcccgga ccatttgata
    41221 ccagagaaca ctaacgatgg attcgcaacg caatctttta gtcatcgctt tgctgttcgt
    41281 gtctttcatg atctggcaag cctgggagca ggataaaaac ccgcaacctc aggcccaaca
    41341 gaccacgcag acaacgacca ccgcagcggg tagcgccgcc aaccagggcg taccggccag
    41401 tggccagggg aaactgatct cggttaagac cgacgtgctt gatctgacca tcaacacccg
    41461 tggtggtgat gttgagcaag ctctgctgcc tgcttacccg aaagagctga actctaccca
    41521 gccgttccag ctgttggaaa cttcaccgca gtttatttat caggcacaga gcggtctgac
    41581 cggtcgtgat ggcccggata acccggctaa cggcccgcgt ccgctgtata acgttgaaaa
    41641 agacgcttat gtgctggctg aaggtcaaaa cgaactgcag gtgccgatga cgtataccga
    41701 cgcggcaggc aacacgttta ccaaaacgtt tgtcctgaaa cgtggtgatt acgctgtcaa
    41761 cgtcaactac aacgtgcaga acgctggcga gaaaccgctg gaaatctcct cgtttggtca
    41821 gttgaagcaa tccatcactc tgccaccgca tctcgatacc ggaagcagca acttcgcact
    41881 gcacaccttc cgtggcgcgg cgtactccac gcctgacgag aagtatgaga aatacaagtt
    41941 cgataccatt gccgataacg aaaacctgaa catctcttcg aaaggtggtt gggtggcgat
    42001 gctgcaacag tatttcgcga cggcgtggat cccgcataac gacggtacca acaacttcta
    42061 taccgctaat ctgggtaacg gcatcgccgc tatcggctat aaatctcagc cggtactggt
    42121 tcagcctggt cagactggcg cgatgaacag caccctgtgg gttggcccgg aaatccagga
    42181 caaaatggca gctgttgctc cgcacctgga tctgaccgtt gattacggtt ggttgtggtt
    42241 catctctcag ccgctgttca aactgctgaa atggatccat agctttgtgg gtaactgggg
    42301 cttctccatt atcatcatca cctttatcgt tcgtggcatc atgtacccgc tgaccaaagc
    42361 gcagtacacc tccatggcga agatgcgtat gctgcagccg aagattcagg caatgcgtga
    42421 gcgtctgggc gatgacaaac agcgtatcag ccaggaaatg atggcgctgt acaaagctga
    42481 gaaggttaac ccgctgggcg gctgcttccc gctgctgatc cagatgccaa tcttcctggc
    42541 gttgtactac atgctgatgg gttccgttga actgcgtcag gcaccgtttg cactgtggat
    42601 ccacgacctg tcggcacagg acccgtacta catcctgccg atcctgatgg gcgtaacgat
    42661 gttcttcatt cagaagatgt cgccgaccac agtgaccgac ccgatgcagc agaagatcat
    42721 gacctttatg ccggtcatct tcaccgtgtt cttcctgtgg ttcccgtcag gtctggtgct
    42781 gtactatatc gtcagcaacc tggtaaccat tattcagcag cagctgattt accgtggtct
    42841 ggaaaaacgt ggcctgcata gccgcgagaa gaaaaaatcc tgattcggtg agttttcgct
    42901 aaaataaggg cggtcagttg accgcctttt ttcttttcgt agggtggata agcaccgcgc
    42961 atccgccaca caaagcaaca ggaacatcat gagcgataat gacactatcg tagcccaggc
    43021 cacgcctccg ggacgtggcg gcgttggcat cctgcgcatc tccggcctca aagcccgtga
    43081 agttgccgaa accgtgctgg gtaaactgcc taagccgcgc tacgccgatt atcttccgtt
    43141 taaagacgcc gacggcagcg tgctcgatca ggggattgcg ctatggttcc ctggcccgaa
    43201 ctcgttcacc ggcgaagatg tgctggaact gcagggtcat ggcggtccgg tgatcctcga
    43261 cctgctgtta aaacgcattc tgaccattcc cggcctgcgg attgctcgcc ctggtgagtt
    43321 ttccgaacgc gcgtttctta acgataaact cgacttagcc caggccgagg cgattgccga
    43381 tcttatcgac gccagttcgg aacaggcggc ccgttcggca cttaactcgc tgcaaggcgc
    43441 attctccgca cgggtcaacc atctggtgga agccctcacc cacttgcgca tttacgtcga
    43501 agcggcaatt gatttccctg atgaagagat cgatttcctc tccgacggca aaattgaagc
    43561 ccagcttaat gacgtgattg ccgatctcga tgcagtgcgt gctgaagcac gtcagggtag
    43621 tttgttgcgc gaagggatga aggtggtgat tgccggacgt cctaacgccg gtaaatcgag
    43681 cctgttaaac gcgctggcgg gccgtgaagc agcaatcgta actgatatcg ccggaaccac
    43741 acgtgacgtg ctgcgtgagc atatccacat tgacggaatg ccgctgcata tcatcgatac
    43801 cgctgggcta cgtgaagcca gtgacgaagt ggaacgtatt ggtatcgagc gcgcgtggca
    43861 ggaaattgaa caggccgacc gcgtgctgtt tatggtcgat ggcaccacaa cagacgccgt
    43921 ggatccggca gagatctggc cggaatttat cgcccgtctg ccagcgaaac tgccgatcac
    43981 cgtggtgcgc aataaagccg atatcaccgg cgaaacgctg ggaatgagtg aagtgaacgg
    44041 tcacgcgtta attcgtctct cggcaaggac aggtgaaggc gtggaggtgc tgcgtaacca
    44101 tctcaaacag agcatgggct ttgacaccaa catggaaggc ggcttcctgg cgcgtcgtcg
    44161 ccacctacag gcgctggaac aggcagcgga gcatttgcag caaggtaaag cgcaactgtt
    44221 aggtgcctgg gcgggtgaac tgctggcgga agagttgcgc ctggcacagc agaacttaag
    44281 cgaaatcacc ggggaattta cttcagacga cctgctgggg cggattttct ccagcttctg
    44341 tattggtaag taaccgcgct tacgaagccg tattctgact gtcagatgcg gcttcgcttc
    44401 attgttagca ctcctgttat tcctcaaccc tttttttaaa cattaaaatt cttacgtaat
    44461 ttataatctt aaaaaaaagc atttaatatt gctccccgaa cgattgtgat tcgattcaca
    44521 tttaaacaat tttagaatag acaaaattct tgagtattat actgtagcct cgtgtctttc
    44581 gaggataagt gcattatgaa tatcttacat atatgtgtga cctcaaaatg gttcaatatc
    44641 gacaataaga ttgtcgatca ccgcccttaa tttacccttc tgtagccatc accagagcca
    44701 aaccgattag attcaatgtg aacgattagt ttgctatatc ttaattttgc cttttgcaaa
    44761 ggccatctct cgtttattta cttattttag taaatgatgg tgcttgtata tatacatggc
    44821 gaattaatgg atattgcaga tgtaatattc acagggatca ctgtaattaa aataaatgaa
    44881 ggattatgta atggaaaact ttaaacatct ccctgaaccg ttccgcattc gtgttattga
    44941 gccagtaaaa cgtactactc gcgcttatcg tgaagaagca attattaaat ccggtatgaa
    45001 cccgttcctg ctggatagcg aagatgtgtt tatcgattta ctgaccgaca gcggcaccgg
    45061 ggcggtaacc caaagtatgc aggcagcgat gatgcgcggc gacgaagcct acagcggcag
    45121 ccgcagctac tatgcgttag ccgagtcagt gaaaaatatc tttggttatc aatatactat
    45181 tccgactcac cagggccgtg gcgcagagca aatctatatt ccggtactga ttaaaaaacg
    45241 cgagcaggaa aaaggcctgg atcgcagcaa aatggtggca ttctctaact atttctttga
    45301 taccacgcag ggccatagcc agattaacgg ctgtaccgtg cgtaacgtct atatcaaaga
    45361 agccttcgat acgggcgtgc gttacgactt taaaggcaac tttgacctcg aaggattaga
    45421 acgcggtatt gaagaagttg gcccgaataa cgtgccgtat atcgttgcaa ccatcaccag
    45481 taactctgca ggtggtcagc cggtttcact ggcaaactta aaagcgatgt acagcatcgc
    45541 gaagaaatac gatattccgg tggtaatgga ctccgcacgc tttgctgaaa acgcctattt
    45601 catcaagcag cgtgaagcag aatacaaaga ctggaccatc gagcagatca cccgcgaaac
    45661 ctacaaatat gccgatatgc tggcgatgtc cgccaagaaa gatgcgatgg tgccgatggg
    45721 cggcttgctg tgcgtgaaag acgacagctt ctttgatgtg tacaccgagt gcagaaccct
    45781 ttgcgtggtg caggaaggct tcccgacata tggcggcctg gaaggcggcg cgatggagcg
    45841 tctggcggta ggtctgtatg acggcatgaa tctcgactgg ctggcttatc gtatcgcgca
    45901 ggtacagtat ctggtcgatg gtctggaaga gattggcgtt gtctgccagc aggcgggcgg
    45961 tcacgcggca ttcgttgatg ccggtaaact gctgccgcat atcccggcag accagttccc
    46021 ggcacaggcg ctggcgtgcg agctgtataa agtcgccggt atccgtgcgg tagaaattgg
    46081 ctctttcctg ttaggccgcg atccgaaaac cggtaaacaa ctgccatgcc cggctgaact
    46141 gctgcgttta accattccgc gcgcaacata tactcaaaca catatggact tcattattga
    46201 agcctttaaa catgtaaaag agaacgcggc gaatattaaa ggattaacct ttacctacga
    46261 accaaaagta ttgcgtcact tcaccgcaaa actgaaagaa gtttaattaa tactacagag
    46321 tggctataag gatgttagcc actctcttac cctacatcct caataacaaa aatagccttc
    46381 ctctaaaggt ggcatcatga ctgatcaagc tgaaaaaaag cactctgcat tttggggtgt
    46441 tatggttata gcaggtacag taattggtgg aggtatgttt gctttacctg ttgatcttgc
    46501 cggtgcctgg tttttctggg gtgcctttat ccttatcatt gcctggtttt caatgcttca
    46561 ttccgggtta ttgttattag aagcaaattt aaattatcct gtcggctcca gttttaacac
    46621 catcaccaaa gatttaatcg gtaacacctg gaacattatc agcggtatta ccgttgcctt
    46681 cgttctctat atcctcactt atgcctatat atctgctaat ggtgcgatca ttagtgaaac
    46741 gatatcaatg aatttgggct atcacgctaa tccacgtatt gtcgggatct gcacagccat
    46801 tttcgttgcc agcgtattgt ggataagttc gttagccgcc agtcgcatta cctcattgtt
    46861 cctcgggctg aagattatct cctttgtgat cgtgtttggt tctttcttct tccaggtcga
    46921 ttactccatc ctgcgcgatg ccaccagcac cactgcggga acgtcttact tcccgtatat
    46981 ctttatggct ttgccggtgt gtctggcgtc atttggtttc cacggcaata ttcccagcct
    47041 gattatttgc tatggaaaac gcaaagataa gttaatcaaa agcgtggtat ttggttcgct
    47101 gctggcgctg gtgatttatc tcttctggct ctattgcacg atggggaata ttccgcgcga
    47161 aagctttaag gcgataatct cctcaggcgg caacgttgat tcgctagtga aatcgttcct
    47221 cggcaccaaa cagcacggca ttatcgagtt ttgcctgctg gtgttctcta acttagctgt
    47281 tgccagttcg ttctttggtg tcacgctggg gttgttcgat tatctggcgg acctgtttaa
    47341 gattgataac tcccacggcg ggcgtttcaa aaccgtgctg ttaaccttcc tgccacctgc
    47401 gttgttgtat ctgatcttcc cgaacggctt tatttacggg atcggcggtg ccgggctgtg
    47461 cgccaccatc tgggcggtca ttattcccgc agtgcttgca atcaaagctc gcaagaagtt
    47521 tcccaatcag atgttcacgg tctggggcgg caatcttatt ccggcgattg tcattctctt
    47581 tggtataacc gtgattttgt gctggttcgg caacgtcttt aacgtgttac ctaaatttgg
    47641 ctaaatcctt caagaagcca gccattcgct ggcttcttgc ctctcaggaa atcacttatg
    47701 tccaaatggc aactcgcctg atcctccttc accacgtatg ctttgcgtca ccttactatc
    47761 aggacgcttt agcccatgtc ccgctttttg atttgtagtt ttgccctggt tttactttat
    47821 cccgccggga ttgatatgta cctcgttggt ttaccgcgca tcgccgccga tctcaatgcc
    47881 agcgaagcgc agttgcatat tgcgttctcc gtatatctgg cggggatggc agctgcgatg
    47941 ttatttgccg gtaaagtggc cgatcgttca gggagaaagc cggtcgccat acccggcgcg
    48001 gcgctattta ttattgcctc ggtgttctgt tcactggctg aaaccagcac gttatttctt
    48061 gcaggccgat ttctacaggg gttgggcgca ggctgttgtt acgtagtggc gttcgccatt
    48121 ttgcgcgaca cgctggatga tcgacgtcgg gctaaagtgc tgtcattact caacggtatt
    48181 acctgcatca ttccggtgtt agcgccagtg ctcggacatc tgattatgct taaattcccg
    48241 tggcagagtc tgttctgggc gatggcaatg atgggcatcg cggtactgat gttgtctttg
    48301 tttattttaa aagaaacgcg cccagcgtcc cccgctgctt cggacaaacc acgagaaaat
    48361 agcgagtcgc tgcttaatcg gtttttcctc agccgtgttg ttatcaccac cctcagcgtt
    48421 tcggtgatcc tcactttcgt caatacatcg ccggtattgc tgatggaaat catgggtttt
    48481 gagcgcggag aatacgccac cattatggcg ttgaccgctg gcgtcagcat gaccgtttca
    48541 ttctccacgc catttgcgct gggaattttt aagccacgta cgttgatgat cacctcgcag
    48601 gtgttattcc ttgcagcggg gatcaccctt gccgtttcac cttcccatgc ggtttctctg
    48661 tttggtatca cgctgatttg cgccggtttc tcggtaggtt ttggcgtagc gatgagtcag
    48721 gcgttaggac cattttcatt acgcgcgggc gtagccagct cgaccttagg tattgcgcag
    48781 gtttgcggtt cgtcactgtg gatttggctg gcagcggtgg ttggtatcgg cgcatggaat
    48841 atgctgatcg ggattctgat tgcctgtagc atagtgagcc tgttgctgat tatgttcgtc
    48901 gcgcctggac gccccgttgc cgctcatgaa gaaatccatc accacgcttg atctcaatct
    48961 gctgctctgt ctgcaactgc tgatgcagga gcgcagcgtg accaaagcgg cgaagcggat
    49021 gaacgtgacc ccttcagcgg tgagtaagtc gctggcaaag ttaagagcgt ggtttgacga
    49081 cccgctcttt gtgaactcac cgctggggct gtcgcccaca ccgctaatgg tcagcatgga
    49141 gcaaaatctg gcggaatgga tgcaaatgag caatctgctg ctggataaac cgcaccacca
    49201 gacaccgcgc ggcctgaagt ttgagttggc ggcggaatcg ccgctgatga tgatcatgct
    49261 taatgcgctg tcgaaacgga tctaccaacg ttacccgcag gcgaccatca aactacgtaa
    49321 ctgggattac gattccttag atgccattac tcgtggtgaa gtggatattg gtttttccgg
    49381 tcgcgaaagc catcctcgct cgcgggagct gttaagctcg ctaccgttag ccattgatta
    49441 tgaagtgctg tttagtgatg taccctgcgt ctggttacgc aaagatcatc cggcactgca
    49501 tgaaacgtgg aatctggaca cctttttacg ttatccgcat atcagcattt gctgggaaca
    49561 gagcgacacc tgggcgctgg acaatgtgtt acaggagctg gggcgcgaac gcacgattgc
    49621 tatgagccag ccggaattcg agcagtcact gtttatggca gcgcaacccg acaatctgct
    49681 actggcaacc gcgccgcgct actgtcagta ctacaatcaa ctccaccaac tgccgttggt
    49741 tgcgcttcct ctcccgtttg actaaagcca gcaaaaaaag ctggaagtcc cttttaccca
    49801 gctttggcat aaacggaaca gccataatcc gaagatcgtc tggttacggg aaaccattaa
    49861 aaacctttac gcgtcgatgg cataaccgaa tcgtatgaaa cgcgacccaa tttcacaata
    49921 aaatgtaaaa aagttgtaat aagcttgtct gaatcgaact tttagccgct ttagtctgtc
    49981 catcattcca gtaaatgatt actcttgtat tcataatgga ccattaagca tggagcgaaa
    50041 aatggcgact cactttgccc gagggatttt aacggaagga catctgattt ctgttcgtct
    50101 cccctcccag tgtcatcaag aagcccgaaa cattccccct catcgtcaaa gccgttttct
    50161 ggcgtccaga ggtttactcg cagaactgat gttcatgctg tatggcattg gcgaattgcc
    50221 ggaaatcgtc accctgccga aaggtaaacc ggttttcagt gataaaaatt tgccttcgtt
    50281 ttctatttcc tatgccggga atatggttgg cgtggcgtta acaaccgaag gtgaatgtgg
    50341 cctcgatatg gaactacagc gtgcgacgcg cgggtttcat agcccacacg cgcccgataa
    50401 ccacaccttt tccagcaatg aatcgctatg gatcagtaaa caaaacgatc ctaacgaagc
    50461 gcgggcgcag ctcatcacgc tgcgccgaag cgtgctaaaa ctaaccggtg atgttttgaa
    50521 tgacgatccg cgcgatctgc agctgctgcc cattgccgga cgcctgaaat gtgctcatgt
    50581 aaatcatgta gaagcgttat gcgacgcgga ggacgtgctg gtgtggtccg tggcggtcac
    50641 gcccgcgatt gaaaagctca gtgtctggga gttagatggc aaacacagct ggaaaagcct
    50701 gccggatatt cacagccgcg ccaacaatcc taccagccgg atgatgcgtt ttgcccagct
    50761 ctctaccgtg aagtcttttt cgccaaattg atagacaacc acaggagtca ttatgtctga
    50821 aaaattgcag gtggttacgt tactggggag cctgcgcaaa ggctcattta atggcatggt
    50881 tgcacgtacc ctgccgaaaa ttgctccggc gagcatggaa gtcaatgcgt taccttccat
    50941 tgccgacatt cccttgtatg acgctgacgt acagcaggaa gaaggttttc cagcaacggt
    51001 tgaagctctg gcgaaacaga tccgtcaggc tgacggtgtg gtgatcgtca cgccggaata
    51061 taactactcg gtaccgggtg ggctgaaaaa tgccatcgac tggctttccc gcctgccgga
    51121 tcaaccgctg gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg
    51181 cgcgcgctgt cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa
    51241 caagccggaa tttatgggcg gcgtgattca gaacaaagtt gatccgcaaa ccggagaagt
    51301 gattgatcag ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat
    51361 tcagcgagtt aagatctaaa taaaaaaccc gccagcaatc atgcatggcg ggtttttaac
    51421 gcgctatcga ttttagtgag cgtcgataaa gacaatcttc aggataaaca gcagcgcaac
    51481 gatgattacg cacgggctta gatcacgcag acgtccggta ccgattttca tcacgcagta
    51541 ggagataaag cccagcgcga taccttcggt aatcgagaag ctgaacggca tcatcacggc
    51601 ggtaataaac gccggaacag attcagtaag atcctgccag ttcacgcgtg ccagactgga
    51661 agtcatcagc acgccaacgt aaatcagcgc accagctgca gcgtagcctg gcaccatccc
    51721 cgccagcggc gacagaaaga taaccagcag gaacagcaga ccaacaacca ctgccgtcag
    51781 accggtacga ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga
    51841 agtaccgata aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat
    51901 gcgcgggaat ttacccttct catccgccag gcctgcttta tcggtcacgc caatcagcgt
    51961 accggaggag tcaaacaggt tgaccagcat gaaagagaaa atcacccctg ccagcccgag
    52021 gttaaacgac ccggctaaat ctacatggcc cacgacagtc attacgctcg gcggcgcaga
    52081 aacgatgcca ttgtagtgga catcacccag catccagccc agcagcgttg tcaccacgat
    52141 ggaaaccagc accgccgcgt gaatgttgcg cgaggccaga atagcgatga tgaagaagcc
    52201 gaggatcccc agcagcacgc tgtgagaagt caggttaccg atgctgacca gcgtttccgg
    52261 gttagcgaca atcacacctg cattcttcag tcccatcata ccaatgaaca gaccgatacc
    52321 gctggtgatg cccacacgca gacttaccgg aatgttggca atcatccagt agcgaacgcg
    52381 gaaaatcgtc agtaacagca gaccaatcgc accccagaag attgcgccca tcccgacttg
    52441 ccacggcaag cccatcgcct gcacaaccac aaaagcgaag aacgcattca ggcccatagc
    52501 gggtgccagt gcaactggca ggttagcaaa cagtcccatc ataatactgc cgaatgcagc
    52561 aatcagacag gtagtaacga agacggcgct ggtatccatg ccagcaacgc caagaatttg
    52621 cgggttaaca aaaacgatgt aaaccatcgt caggaaggtg gtaaaaccgg cgatcacttc
    52681 ggtccgtgcc gtcgtgccat gttcgcgcag tttaaacacg cgttccagca tcccctggcc
    52741 agaagcctgg gttgtgtgtt gttgactcat tatctatttc cgaacaagga gggaaaatcc
    52801 gtcgctatcg tataccaaaa tgagacaata tggacggctg aaggtaactt tttttcattg
    52861 tatttgctta tacggcaacg attgcgtcat gcgaattggc atgacgaaaa cgttaaacta
    52921 acgataaagg aaagacatgt cccagataga agcggtattt ttcgactgcg acggtacgct
    52981 ggtcgatagc gaggtcattt gctctcgcgc atatgtaacg atgtttcagg aatttggtat
    53041 tacgctcgat cctgaagagg tattcaaacg tttcaaaggt gtaaaactgt acgaaattat
    53101 cgatattgtt tcccttgaac atggtgttac gttagcgaaa acagaagctg aacacgttta
    53161 ccgtgcagaa gtcgctcggc tgttcgattc agaactggaa gccatcgaag gggctggagc
    53221 gctcctgtca gcgatcactg cgccaatgtg tgtggtatct aacggcccaa ataacaaaat
    53281 gcagcattct atgggcaagc tgaatatgtt
//
LOCUS       DAZKHC020000021        52656 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZKHC020000021 DAZKHC020000000
VERSION     DAZKHC020000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 52656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.021"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            203..1150
                     /gene="cyoA"
                     /locus_tag="ACHOSM_RS07500"
                     /old_locus_tag="pgaptmp_001500"
     CDS             203..1150
                     /gene="cyoA"
                     /locus_tag="ACHOSM_RS07500"
                     /old_locus_tag="pgaptmp_001500"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HGU4558051.1"
                     /translation="MRLRKYNKSLGWLSLYAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1172..3163
                     /gene="cyoB"
                     /locus_tag="ACHOSM_RS07505"
                     /old_locus_tag="pgaptmp_001501"
     CDS             1172..3163
                     /gene="cyoB"
                     /locus_tag="ACHOSM_RS07505"
                     /old_locus_tag="pgaptmp_001501"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HGU4558052.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3153..3767
                     /gene="cyoC"
                     /locus_tag="ACHOSM_RS07510"
                     /old_locus_tag="pgaptmp_001502"
     CDS             3153..3767
                     /gene="cyoC"
                     /locus_tag="ACHOSM_RS07510"
                     /old_locus_tag="pgaptmp_001502"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HGU4558053.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGVTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3767..4096
                     /gene="cyoD"
                     /locus_tag="ACHOSM_RS07515"
                     /old_locus_tag="pgaptmp_001503"
     CDS             3767..4096
                     /gene="cyoD"
                     /locus_tag="ACHOSM_RS07515"
                     /old_locus_tag="pgaptmp_001503"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HGU4558054.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4108..4998
                     /gene="cyoE"
                     /locus_tag="ACHOSM_RS07520"
                     /old_locus_tag="pgaptmp_001504"
     CDS             4108..4998
                     /gene="cyoE"
                     /locus_tag="ACHOSM_RS07520"
                     /old_locus_tag="pgaptmp_001504"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HGU4558055.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5183..5494
                     /locus_tag="ACHOSM_RS07525"
                     /old_locus_tag="pgaptmp_001505"
     CDS             5183..5494
                     /locus_tag="ACHOSM_RS07525"
                     /old_locus_tag="pgaptmp_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HGU4558056.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNLLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            5478..6020
                     /locus_tag="ACHOSM_RS07530"
                     /old_locus_tag="pgaptmp_001506"
     CDS             5478..6020
                     /locus_tag="ACHOSM_RS07530"
                     /old_locus_tag="pgaptmp_001506"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4558057.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(6076..7011)
                     /locus_tag="ACHOSM_RS07535"
                     /old_locus_tag="pgaptmp_001507"
     CDS             complement(6076..7011)
                     /locus_tag="ACHOSM_RS07535"
                     /old_locus_tag="pgaptmp_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4558058.1"
                     /translation="MLTKRILFSVMLIVSPSMVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            7419..8783
                     /gene="yajR"
                     /locus_tag="ACHOSM_RS07540"
                     /old_locus_tag="pgaptmp_001508"
     CDS             7419..8783
                     /gene="yajR"
                     /locus_tag="ACHOSM_RS07540"
                     /old_locus_tag="pgaptmp_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4558059.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAVLSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKDSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPA
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(8911..9402)
                     /gene="yajQ"
                     /locus_tag="ACHOSM_RS07545"
                     /old_locus_tag="pgaptmp_001509"
     CDS             complement(8911..9402)
                     /gene="yajQ"
                     /locus_tag="ACHOSM_RS07545"
                     /old_locus_tag="pgaptmp_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HGU4558060.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            9570..10481
                     /gene="panE"
                     /locus_tag="ACHOSM_RS07550"
                     /old_locus_tag="pgaptmp_001510"
     CDS             9570..10481
                     /gene="panE"
                     /locus_tag="ACHOSM_RS07550"
                     /old_locus_tag="pgaptmp_001510"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HGU4558061.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNGVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            10444..11034
                     /gene="yajL"
                     /locus_tag="ACHOSM_RS07555"
                     /old_locus_tag="pgaptmp_001511"
     CDS             10444..11034
                     /gene="yajL"
                     /locus_tag="ACHOSM_RS07555"
                     /old_locus_tag="pgaptmp_001511"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HGU4558062.1"
                     /translation="MSASALVCLAPGSEETEAVTTIALLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(11088..12536)
                     /gene="thiI"
                     /locus_tag="ACHOSM_RS07560"
                     /old_locus_tag="pgaptmp_001512"
     CDS             complement(11088..12536)
                     /gene="thiI"
                     /locus_tag="ACHOSM_RS07560"
                     /old_locus_tag="pgaptmp_001512"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGU4558063.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            12742..12984
                     /gene="xseB"
                     /locus_tag="ACHOSM_RS07565"
                     /old_locus_tag="pgaptmp_001513"
     CDS             12742..12984
                     /gene="xseB"
                     /locus_tag="ACHOSM_RS07565"
                     /old_locus_tag="pgaptmp_001513"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGU4558064.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            12984..13883
                     /gene="ispA"
                     /locus_tag="ACHOSM_RS07570"
                     /old_locus_tag="pgaptmp_001514"
     CDS             12984..13883
                     /gene="ispA"
                     /locus_tag="ACHOSM_RS07570"
                     /old_locus_tag="pgaptmp_001514"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HGU4558065.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            13908..15770
                     /gene="dxs"
                     /locus_tag="ACHOSM_RS07575"
                     /old_locus_tag="pgaptmp_001515"
     CDS             13908..15770
                     /gene="dxs"
                     /locus_tag="ACHOSM_RS07575"
                     /old_locus_tag="pgaptmp_001515"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HGU4558066.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            15825..16799
                     /gene="yajO"
                     /locus_tag="ACHOSM_RS07580"
                     /old_locus_tag="pgaptmp_001516"
     CDS             15825..16799
                     /gene="yajO"
                     /locus_tag="ACHOSM_RS07580"
                     /old_locus_tag="pgaptmp_001516"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HGU4558067.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     VSQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(16853..17371)
                     /gene="pgpA"
                     /locus_tag="ACHOSM_RS07585"
                     /old_locus_tag="pgaptmp_001517"
     CDS             complement(16853..17371)
                     /gene="pgpA"
                     /locus_tag="ACHOSM_RS07585"
                     /old_locus_tag="pgaptmp_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HGU4558068.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(17349..18326)
                     /gene="thiL"
                     /locus_tag="ACHOSM_RS07590"
                     /old_locus_tag="pgaptmp_001518"
     CDS             complement(17349..18326)
                     /gene="thiL"
                     /locus_tag="ACHOSM_RS07590"
                     /old_locus_tag="pgaptmp_001518"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HGU4558069.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(18404..18823)
                     /gene="nusB"
                     /locus_tag="ACHOSM_RS07595"
                     /old_locus_tag="pgaptmp_001519"
     CDS             complement(18404..18823)
                     /gene="nusB"
                     /locus_tag="ACHOSM_RS07595"
                     /old_locus_tag="pgaptmp_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGU4558070.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(18843..19313)
                     /gene="ribE"
                     /locus_tag="ACHOSM_RS07600"
                     /old_locus_tag="pgaptmp_001520"
     CDS             complement(18843..19313)
                     /gene="ribE"
                     /locus_tag="ACHOSM_RS07600"
                     /old_locus_tag="pgaptmp_001520"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGU4558071.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(19402..20505)
                     /gene="ribD"
                     /locus_tag="ACHOSM_RS07605"
                     /old_locus_tag="pgaptmp_001521"
     CDS             complement(19402..20505)
                     /gene="ribD"
                     /locus_tag="ACHOSM_RS07605"
                     /old_locus_tag="pgaptmp_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGU4558072.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGAIAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQLLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPIRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(20509..20958)
                     /gene="nrdR"
                     /locus_tag="ACHOSM_RS07610"
                     /old_locus_tag="pgaptmp_001522"
     CDS             complement(20509..20958)
                     /gene="nrdR"
                     /locus_tag="ACHOSM_RS07610"
                     /old_locus_tag="pgaptmp_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGU4558073.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            21109..21648
                     /gene="yajI"
                     /locus_tag="ACHOSM_RS07615"
                     /old_locus_tag="pgaptmp_001523"
     CDS             21109..21648
                     /gene="yajI"
                     /locus_tag="ACHOSM_RS07615"
                     /old_locus_tag="pgaptmp_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU4558074.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            21947..22831
                     /gene="tsx"
                     /locus_tag="ACHOSM_RS07620"
                     /old_locus_tag="pgaptmp_001524"
     CDS             21947..22831
                     /gene="tsx"
                     /locus_tag="ACHOSM_RS07620"
                     /old_locus_tag="pgaptmp_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HGU4558075.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(23008..23355)
                     /gene="yajD"
                     /locus_tag="ACHOSM_RS07625"
                     /old_locus_tag="pgaptmp_001525"
     CDS             complement(23008..23355)
                     /gene="yajD"
                     /locus_tag="ACHOSM_RS07625"
                     /old_locus_tag="pgaptmp_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HGU4558076.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(23484..24455)
                     /gene="secF"
                     /locus_tag="ACHOSM_RS07630"
                     /old_locus_tag="pgaptmp_001526"
     CDS             complement(23484..24455)
                     /gene="secF"
                     /locus_tag="ACHOSM_RS07630"
                     /old_locus_tag="pgaptmp_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HGU4558077.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(24466..26313)
                     /gene="secD"
                     /locus_tag="ACHOSM_RS07635"
                     /old_locus_tag="pgaptmp_001527"
     CDS             complement(24466..26313)
                     /gene="secD"
                     /locus_tag="ACHOSM_RS07635"
                     /old_locus_tag="pgaptmp_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HGU4558078.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(26341..26673)
                     /gene="yajC"
                     /locus_tag="ACHOSM_RS07640"
                     /old_locus_tag="pgaptmp_001528"
     CDS             complement(26341..26673)
                     /gene="yajC"
                     /locus_tag="ACHOSM_RS07640"
                     /old_locus_tag="pgaptmp_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGU4558079.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(26696..27823)
                     /gene="tgt"
                     /locus_tag="ACHOSM_RS07645"
                     /old_locus_tag="pgaptmp_001529"
     CDS             complement(26696..27823)
                     /gene="tgt"
                     /locus_tag="ACHOSM_RS07645"
                     /old_locus_tag="pgaptmp_001529"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGU4558080.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(27879..28949)
                     /gene="queA"
                     /locus_tag="ACHOSM_RS07650"
                     /old_locus_tag="pgaptmp_001530"
     CDS             complement(27879..28949)
                     /gene="queA"
                     /locus_tag="ACHOSM_RS07650"
                     /old_locus_tag="pgaptmp_001530"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGU4558081.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLFDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            29042..29623
                     /gene="acpH"
                     /locus_tag="ACHOSM_RS07655"
                     /old_locus_tag="pgaptmp_001531"
     CDS             29042..29623
                     /gene="acpH"
                     /locus_tag="ACHOSM_RS07655"
                     /old_locus_tag="pgaptmp_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HGU4558082.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(29628..31445)
                     /gene="malZ"
                     /locus_tag="ACHOSM_RS07660"
                     /old_locus_tag="pgaptmp_001532"
     CDS             complement(29628..31445)
                     /gene="malZ"
                     /locus_tag="ACHOSM_RS07660"
                     /old_locus_tag="pgaptmp_001532"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HGU4558083.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(31601..32974)
                     /gene="proY"
                     /locus_tag="ACHOSM_RS07665"
                     /old_locus_tag="pgaptmp_001533"
     CDS             complement(31601..32974)
                     /gene="proY"
                     /locus_tag="ACHOSM_RS07665"
                     /old_locus_tag="pgaptmp_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HGU4558084.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(33050..34369)
                     /gene="brnQ"
                     /locus_tag="ACHOSM_RS07670"
                     /old_locus_tag="pgaptmp_001534"
     CDS             complement(33050..34369)
                     /gene="brnQ"
                     /locus_tag="ACHOSM_RS07670"
                     /old_locus_tag="pgaptmp_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HGU4558085.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(34776..36071)
                     /gene="phoR"
                     /locus_tag="ACHOSM_RS07675"
                     /old_locus_tag="pgaptmp_001535"
     CDS             complement(34776..36071)
                     /gene="phoR"
                     /locus_tag="ACHOSM_RS07675"
                     /old_locus_tag="pgaptmp_001535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HGU4558086.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(36129..36818)
                     /gene="phoB"
                     /locus_tag="ACHOSM_RS07680"
                     /old_locus_tag="pgaptmp_001536"
     CDS             complement(36129..36818)
                     /gene="phoB"
                     /locus_tag="ACHOSM_RS07680"
                     /old_locus_tag="pgaptmp_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HGU4558087.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            37008..38210
                     /gene="sbcD"
                     /locus_tag="ACHOSM_RS07685"
                     /old_locus_tag="pgaptmp_001537"
     CDS             37008..38210
                     /gene="sbcD"
                     /locus_tag="ACHOSM_RS07685"
                     /old_locus_tag="pgaptmp_001537"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HGU4558088.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            38207..41350
                     /gene="sbcC"
                     /locus_tag="ACHOSM_RS07690"
                     /old_locus_tag="pgaptmp_001538"
     CDS             38207..41350
                     /gene="sbcC"
                     /locus_tag="ACHOSM_RS07690"
                     /old_locus_tag="pgaptmp_001538"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HGU4558089.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            41475..42659
                     /gene="araJ"
                     /locus_tag="ACHOSM_RS07695"
                     /old_locus_tag="pgaptmp_001539"
     CDS             41475..42659
                     /gene="araJ"
                     /locus_tag="ACHOSM_RS07695"
                     /old_locus_tag="pgaptmp_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HGU4558090.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTPGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(42802..43710)
                     /gene="mak"
                     /locus_tag="ACHOSM_RS07700"
                     /old_locus_tag="pgaptmp_001540"
     CDS             complement(42802..43710)
                     /gene="mak"
                     /locus_tag="ACHOSM_RS07700"
                     /old_locus_tag="pgaptmp_001540"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HGU4558091.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            43835..44746
                     /gene="rdgC"
                     /locus_tag="ACHOSM_RS07705"
                     /old_locus_tag="pgaptmp_001541"
     CDS             43835..44746
                     /gene="rdgC"
                     /locus_tag="ACHOSM_RS07705"
                     /old_locus_tag="pgaptmp_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HGU4558092.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(44824..44907)
                     /locus_tag="ACHOSM_RS07710"
                     /old_locus_tag="pgaptmp_001542"
     CDS             complement(44824..44907)
                     /locus_tag="ACHOSM_RS07710"
                     /old_locus_tag="pgaptmp_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4558093.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(44904..45245)
                     /locus_tag="ACHOSM_RS07715"
                     /old_locus_tag="pgaptmp_001543"
                     /pseudo
     CDS             complement(44904..45245)
                     /locus_tag="ACHOSM_RS07715"
                     /old_locus_tag="pgaptmp_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(45393..45677)
                     /gene="ppnP"
                     /locus_tag="ACHOSM_RS07720"
                     /old_locus_tag="pgaptmp_001544"
     CDS             complement(45393..45677)
                     /gene="ppnP"
                     /locus_tag="ACHOSM_RS07720"
                     /old_locus_tag="pgaptmp_001544"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HGU4558094.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(45749..46426)
                     /gene="aroM"
                     /locus_tag="ACHOSM_RS07725"
                     /old_locus_tag="pgaptmp_001545"
     CDS             complement(45749..46426)
                     /gene="aroM"
                     /locus_tag="ACHOSM_RS07725"
                     /old_locus_tag="pgaptmp_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HGU4558095.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQHHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(46684..46875)
                     /gene="yaiA"
                     /locus_tag="ACHOSM_RS07730"
                     /old_locus_tag="pgaptmp_001546"
     CDS             complement(46684..46875)
                     /gene="yaiA"
                     /locus_tag="ACHOSM_RS07730"
                     /old_locus_tag="pgaptmp_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HGU4558096.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(46925..47449)
                     /gene="aroL"
                     /locus_tag="ACHOSM_RS07735"
                     /old_locus_tag="pgaptmp_001547"
     CDS             complement(46925..47449)
                     /gene="aroL"
                     /locus_tag="ACHOSM_RS07735"
                     /old_locus_tag="pgaptmp_001547"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HGU4558097.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(47632..48090)
                     /gene="yaiI"
                     /locus_tag="ACHOSM_RS07740"
                     /old_locus_tag="pgaptmp_001548"
     CDS             complement(47632..48090)
                     /gene="yaiI"
                     /locus_tag="ACHOSM_RS07740"
                     /old_locus_tag="pgaptmp_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HGU4558098.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            48210..49019
                     /gene="proC"
                     /locus_tag="ACHOSM_RS07745"
                     /old_locus_tag="pgaptmp_001549"
     CDS             48210..49019
                     /gene="proC"
                     /locus_tag="ACHOSM_RS07745"
                     /old_locus_tag="pgaptmp_001549"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGU4558099.1"
                     /translation="MEKKIGFIGCGNMGKAILSGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(49036..50151)
                     /gene="adrA"
                     /locus_tag="ACHOSM_RS07750"
                     /old_locus_tag="pgaptmp_001550"
                     /gene_synonym="dgcC"
     CDS             complement(49036..50151)
                     /gene="adrA"
                     /locus_tag="ACHOSM_RS07750"
                     /old_locus_tag="pgaptmp_001550"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HGU4558100.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(50253..50573)
                     /gene="psiF"
                     /locus_tag="ACHOSM_RS07755"
                     /old_locus_tag="pgaptmp_001551"
     CDS             complement(50253..50573)
                     /gene="psiF"
                     /locus_tag="ACHOSM_RS07755"
                     /old_locus_tag="pgaptmp_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HGU4558101.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(50692..52107)
                     /gene="phoA"
                     /locus_tag="ACHOSM_RS07760"
                     /old_locus_tag="pgaptmp_001552"
     CDS             complement(50692..52107)
                     /gene="phoA"
                     /locus_tag="ACHOSM_RS07760"
                     /old_locus_tag="pgaptmp_001552"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HGU4558102.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(52208..52468)
                     /gene="iraP"
                     /locus_tag="ACHOSM_RS07765"
                     /old_locus_tag="pgaptmp_001553"
     CDS             complement(52208..52468)
                     /gene="iraP"
                     /locus_tag="ACHOSM_RS07765"
                     /old_locus_tag="pgaptmp_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HGU4558103.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDYDTELLRDYVKKLLKHPRQ"
BASE COUNT        12909 a        13942 c        13203 g        12602 t
ORIGIN      
        1 aactccttct gcactcccct ttcgttataa cgcccttttg caacagcttc ttaaaatcaa
       61 cttgatatgt tttgcaacat atgtgacctg acagccaaat ccaagtaaca ggaatttaat
      121 catgtttaca gtaatgtaac cttcccgtaa aatgcccaca cactttaaac gccaccagat
      181 cccgtggaat tgaggtcgtt aaatgagact caggaaatac aataaaagtt tgggatggtt
      241 gtcattatat gcaggcactg tattgctcag tggctgtaat tctgcgctgt tagatcccaa
      301 aggacagatt ggtctggagc aacgttcact gatactgacg gcatttggcc tgatgttgat
      361 tgtcgttatt cccgcaatct tgatggctgt tggtttcgcc tggaagtacc gtgcgagcaa
      421 taaagatgct aagtacagcc cgaactggtc acactccaat aaagtggaag ctgtggtctg
      481 gacggtacct atcttaatca tcatcttcct tgcggtactg acctggaaaa ccactcacgc
      541 tcttgagcct agcaagccgc tggcacacga cgagaagccc attactatcg aagtggtttc
      601 catggactgg aaatggttct tcatctaccc ggaacagggc attgcaaccg tgaatgaaat
      661 cgctttcccg gcgaacactc cggtgtactt caaagtgacc tccaactccg tgatgaactc
      721 cttcttcatt ccgcgtctgg gtagccagat ttatgccatg gctggtatgc agactcgcct
      781 gcatctgatc gccaacgaac ccggtactta tgacggtatc tccgccagct acagcggccc
      841 gggtttctca ggcatgaagt tcaaagctat tgcaacaccg gatcgcgccg aattcgacca
      901 gtgggtcgca aaagcgaagc agtcgccgaa caccatgtct gacatggctg cgttcgaaaa
      961 actggccgcg cctagcgaat acaaccaggt ggaatatttc tccaacgtga aaccagactt
     1021 gtttgctgat gtgattaaca agtttatggc tcacggtaag agcatggaca tgacccagcc
     1081 agaaggtgag cacagtgcac acgaaggtat ggaaggcatg gacatgagcc acgcggaatc
     1141 cgcccattaa aggggttgag gaagaataaa gatgttcgga aaattatcac ttgatgcagt
     1201 cccgttccat gaacctatcg tcatggttac gatcgctggc attattttgg gaggtctggc
     1261 gctcgttggc ctgatcactt acttcggtaa gtggacctac ctgtggaaag agtggctgac
     1321 ctccgtcgac cataaacgcc tcggtatcat gtatatcatc gtggcgattg tgatgttgct
     1381 gcgtggtttt gctgacgcca ttatgatgcg tagccagcag gctcttgcct cggcgggcga
     1441 agcgggcttc ctgccacctc accactacga tcagatcttc accgcgcacg gcgtgattat
     1501 gatcttcttc gtggcgatgc ctttcgttat cggtctgatg aacctggtgg ttccgctgca
     1561 gatcggcgcg cgtgacgttg cgttcccgtt cctcaacaac ttaagcttct ggtttactgt
     1621 tgttggtgtg attctggtta acgtttctct cggcgtgggc gaatttgcgc agaccggctg
     1681 gctggcctat ccaccgctat cgggaataga gtacagtccg ggagtcggtg tcgattactg
     1741 gatatggagt ctccagctat ccggtatagg tacgacgctt accggtatca acttcttcgt
     1801 taccattctg aagatgcgcg caccgggcat gaccatgttc aagatgccag tatttacctg
     1861 ggcatcactg tgcgcgaacg tactgattat tgcttccttc ccaattctga cggttaccgt
     1921 cgcgttgttg accctggatc gctatctggg cacccatttc tttaccaacg atatgggtgg
     1981 caacatgatg atgtacatca acctgatttg ggcctggggc cacccggaag tttacatcct
     2041 gatcctgcct gttttcggtg tgttctccga aattgcggca accttctcgc gtaaacgtct
     2101 gtttggttat acctcgctgg tatgggcaac cgtctgcatc accgtgctgt cgttcatcgt
     2161 ttggctgcac cacttcttta cgatgggtgc gggcgcgaac gtaaacgcct tctttggtat
     2221 caccacaatg attatcgcca tcccgaccgg ggtgaagatc ttcaactggc tgttcaccat
     2281 gtatcagggc cgcatcgtgt tccattctgc gatgctgtgg actatcggtt ttatcgtcac
     2341 cttctcggtg ggcgggatga ctggcgtgct gctggcagta ccgggcgcgg acttcgttct
     2401 gcataacagc ctgttcctga ttgcgcactt ccataacgtg atcatcggcg gcgtggtctt
     2461 cggctgcttc gcagggatga cctactggtg gcctaaagcg ttcggtttca aactgaacga
     2521 aacctggggt aaacgcgcgt tctggttctg gatcatcggc ttcttcgttg cctttatgcc
     2581 gctgtatgcg ctgggcttca tgggcatgac ccgtcgtttg agccagcaga ttgacccgca
     2641 gttccacacc atgctgatga ttgcagccag cggtgcagta ctgattgcgc tgggtattct
     2701 ctgcctcgtt attcagatgt acgtttctat tcgcgaccgc gaccagaacc gtgacctgac
     2761 tggcgacccg tggggtggcc gtacgctgga gtgggcaacc tcttccccgc ctccgttcta
     2821 taactttgcc gtagtgccgc acgttcacga acgtgatgca ttctgggaaa tgaaagagaa
     2881 aggcgaagcg tataaaaagc ctgaccacta tgaagaaatt catatgccga aaaacagcgg
     2941 tgcaggtatc gtcattgcag ctttctccac catcttcggt ttcgccatga tctggcatat
     3001 ctggtggctg gcgattgttg gcttcgcagg catgatcatc acctggatcg tgaaaagctt
     3061 cgacgaggac gtggattact acgtgccggt ggcagaaatc gaaaaactgg aaaaccagca
     3121 tttcgatgag attactaagg cagggctgaa aaatggcaac tgatactttg acgcacgcga
     3181 ctgcccacgc gcacgaacac gggcaccacg atgcaggcgt aaccaaaatc ttcggatttt
     3241 ggatctacct gatgagcgac tgcattctgt tctcaatctt gtttgctacc tatgccgttc
     3301 tggtgaacgg caccgcaggc ggcccgacag gtaaggacat tttcgaactg ccgttcgttc
     3361 tggttgaaac tttcttgctg ttgttcagct ccatcaccta cggcatggcg gctatcgcca
     3421 tgtacaaaaa caacaaaagc caggttatct cctggctggc gttgacctgg ttgtttggtg
     3481 ccggatttat cgggatggaa atctatgaat tccatcacct gattgttaac ggcatgggtc
     3541 cggatcgcag cggcttcctg tcagcgttct ttgcgctggt cggcacgcac ggtctgcacg
     3601 tcacttctgg tcttatctgg atggcggtgc tgatggtgca aatcgcccgt cgcggcctga
     3661 ccagcactaa ccgtacccgc atcatgtgcc tgagcctgtt ctggcacttc ctggatgtgg
     3721 tttggatctg tgtgttcact gttgtttatc tgatgggggc gatgtaatga gtcattctac
     3781 cgatcacagc ggcgcgtccc atggcagcgt aaaaacctac atgacaggct ttatcctgtc
     3841 gatcattctg acggtgattc cgttctggat ggtgatgaca ggggctgcct ctccggccgt
     3901 aattctggga acaatcctgg caatggcagt ggtacaggtt ctggtgcatc tggtgtgctt
     3961 cctgcacatg aataccaaat cagatgaagg ctggaacatg acggcgtttg tcttcaccgt
     4021 gctaatcatc gctatcctgg ttgtaggctc catctggatt atgtggaacc tcaactacaa
     4081 catgatgatg cactaagagc ggcggttatg atgtttaagc aatacctgca agtaacgaaa
     4141 ccaggcatca tctttggcaa cctgatctcg gtgattgggg gattcctgct ggcctcaaag
     4201 ggcagcattg attatcccct gtttatctac acgctggttg gggtgtcact ggttgtggcg
     4261 tcgggttgtg tgtttaacaa ctacatcgac agggatatcg acagaaagat ggaaaggacg
     4321 aagaatcggg tgctggttaa aggcctgatc tctcctgctg tctcgctggt gtacgccacc
     4381 ttgctgggta ttgctggctt tatgctgctg tggtttggcg cgaatccgct ggcctgctgg
     4441 ctgggggtga tgggctttgt ggtttatgtc ggcgtctata gcctgtacat gaaacgccac
     4501 tcagtctacg gcacattgat tggttcgctc tccggcgctg cgccgccggt gattggctac
     4561 tgtgcagtaa ccggcgagtt cgatagcggc gcagcgatcc tgctggctat cttcagcctg
     4621 tggcagatgc ctcactccta tgccatcgcc attttccgct ttaaggatta ccaagcggca
     4681 aacattccgg tattgccagt ggtaaaaggc atttcggtgg cgaagaatca catcacgctg
     4741 tatatcatcg cctttgccgt tgccacgctg atgctctctc ttggcggtta cgctggatat
     4801 aaatatctgg tggtcgccgc ggcggttagc gtctggtggt taggtatggc tctgcgcggt
     4861 tataaagttg ctgatgacag aatctgggcg cgcaagctgt tcggcttctc tatcatcgcc
     4921 atcactgccc tctcggtgat gatgtccgtt gattttatgg tgccggactc gcatacgctg
     4981 ctggctgctg tttggtaaca caacctctct attaaaaagg tgctacggca ccttttttat
     5041 tgtctgagat cagagatacg gctaataacc gtatgtcttg acagagtgag cagatgaaca
     5101 taaattaatc atacggttga tagccgtatt atgtatgaga tcgactccct acgatttcat
     5161 tcagttaatc aaagaggtca taatgcctgg aaaaacagcc actctacccg atgtcgataa
     5221 aacgctgaaa aatgcgcggg tcgaactcaa aactagtccc gacgcaaaaa acaaacttcg
     5281 tgaagctgcg caagccgttg gggtagattt aagcgctttt attttgagcg ctgctatgga
     5341 acgcgccgaa agtgtgcttg ataaccaacg tcgccgtgag ctttcgaatc aaagctggga
     5401 actgatgaac ctactcatcg ctgaacctgc tcaaccgacg ctcgccctca aggcgttaat
     5461 gaaaaggaaa aacagcgatg gtcgacaagc atgaagagat tactctgccc atagtcctct
     5521 cctgtaatta tcagtctgat attacttatc ctgggcaaaa acagtttgat tgcggtaacc
     5581 ctgttatcga taaatttgta cgcgcatcgc taaagaaaag tgtgcgtaat agcgactgtg
     5641 cggctaaagc acttattgac agacaaagtg gtgaactgat cggcatctgt acttttacgg
     5701 catattcgct ggaaaaacaa cgcgtttctg gcgtccttca gggttcacaa ccttcagaaa
     5761 ttggtgttgt cagattagtc atgttggggg tagcacggaa gtatcaaaag cggggctttg
     5821 gtcaggacct actatgtgat ttttttgaac atgtaaaaat aattcaccag gcattaccaa
     5881 ttaaaggggt ttatcttgat gctgaccctg ccgccattaa tttttatgct cgtctcggct
     5941 ttgttcagct ttcagcgaca ccaaatgctt ttggtgctgt acctatgttt ttggcgattc
     6001 agcatattct cgcggcttag attattatcg ccacccgcca gggcaagttt tgcctcagcc
     6061 gggggctgag cagcgttacc acttttgttc aattgccccc gctttcagca actccacatc
     6121 cttgccgtta atggttgccg ttgcggcaac gattgtggtc cttacagttg cacaagattt
     6181 tcgctcaaat tccggtttat aaagaataag agacgaatta ctggtaaatg aaccaaatag
     6241 gtaatcagta aagctaatat tcagccggtt taaaccgaaa ggcgcgaagc caaccgtttg
     6301 gttaatctcc ttaccggatg ggcgacgaat gctgaatgtc acgcgaagat tggccacttc
     6361 gcctaattta ttggtggtgg cgaagaggct atccatgcag gaaaaacctt tatctaaaaa
     6421 attatctcgc atcccagaac gggtaaccgt aatcacgggt aacgtatcgt caaccgtttt
     6481 tctgtcaatc atgtcttttc gacaagtagc cagaaagtct tccgggccac cataaatgtg
     6541 gacaccttta tcaagatatt ccttcgcttt cttacaatct acctcaacat actgaccatt
     6601 ggcgtacata atgcccaacc tggaccatgc taaattcaca ccgtgctgcg cggcctgatt
     6661 aaatagtgca tacgctttgg cataatccac ccgcacctgc tcaccgtcgt agtagatacc
     6721 ccccagcgct aacggagctt ctctgtcacc ttgtttagcc gctaactcga accatttaat
     6781 cgcttcctga acatcaggtt tggtatcata acgaggctga taatattccc agcctaacag
     6841 gctctgcgcg tcgatatctc ccgattcagc cattttatgc agtgtattta taacatcggg
     6901 agcaggttta ccaccgtata tgctgtcata caattcatgc gctttctcgc tagccaccat
     6961 gcttggtgaa actatcagca ttacagaaaa taatattctt ttagttaaca tgtatacctt
     7021 tatgtcctca ctgtcatata tttccataat aattaaccac caaatatcca tttaatgaat
     7081 aaatacagtg ccataaaggt ccaggtaata agcacacatt tagcctgaaa cattatcatc
     7141 catgtcacca aatcgtttat ttagtgagat aaaactagca tattcgtaag ataaaaaatg
     7201 cacatggcaa tcaagaaatg atattattac ttttttgatg taaaactatt tagttttaaa
     7261 attcattttt tcaacatata gagcattcat tttatgaatg caaaaaaaca tacctgacac
     7321 acaaattact ctcctctgta atattttctc attaacactc tgtttaccca acatgcgctc
     7381 cgcactacac tatgtgggca tttttttaag gggtggtaat gaacgattat aaaatgacgc
     7441 caggtgagag gcgcgcgacc tggggtttag ggaccgtatt ctcgttgcgc atgctgggca
     7501 tgtttatggt tctgccggtt ctgaccacgt acggcatggc tctgcaaggt gccagcgaag
     7561 cattaatcgg tattgccatt ggtatttatg gtctgactca ggccgttttt cagattccgt
     7621 ttggcctgct ttccgaccgt attggtcgca aaccattaat tgtcggtggg ctggcggtgt
     7681 ttgccgccgg tagcgttatc gctgtgctct ctgactccat ctggggaatt attctgggcc
     7741 gggcgctaca aggctccggt gcgattgccg ctgccgttat ggcgctgctt tccgatctca
     7801 cgcgcgaaca aaaccgcacc aaagcaatgg cgtttatcgg cgtgagcttt ggcattacct
     7861 ttgccattgc gatggtgctt ggcccgatca tcactcacaa acttgggctg cacgcgctgt
     7921 tctggatgat cgctattctg gcaacgaccg gcattgcgtt gaccatttgg gttgtgccca
     7981 acagtagcac tcacgtactt aatcgtgagt ccggaatggt gaaagacagt ttcagtaaag
     8041 tgctggcgga accgcggctg ctgaaactca actttggcat tatgtgtctg catattttgc
     8101 tgatgtcgac gtttgttgcc ctgcccggac aactggctga tgcagggttc ccggcggctg
     8161 aacactggaa ggtctatctg gcgacaatgc taatcgcctt tggctcggtc gtgcctttca
     8221 ttatctacgc tgaagttaag cgcaaaatga agcaagtctt tgtcttctgc gtcgggttga
     8281 tcgtggttgc ggaaattgtg ttgtggaacg cgcaaacgca gttctggcaa ctggtggtcg
     8341 gcgtgcagct tttctttgta gcgtttaatt tgatggaagc ccttctgcct tcacttatca
     8401 gtaaagagtc gccagcaggt tacaaaggta cggcgatggg tgtttactcc accagccagt
     8461 ttcttggcgt ggcgattggc ggttcactgg gcggctggat tgacggcatg tttgacggtc
     8521 agggcgtatt tctcgctggc gcaatgctgg ccgcagtgtg gctggcagtc gccagtacca
     8581 tgaaagaacc ggcgtatgtc agcagtttgc gcattgaaat cccggcgaac attgccgcaa
     8641 acgaggcgtt aaaagtgcgt ttgctagaaa ctgaaggcat caaagaagtg ttgattgcag
     8701 aagaagaaca ttcagcttat gtgaaaatcg acagcaaagt gacgaatcgc tttgatgttg
     8761 aacaggcaat tcgccaggca taaaagcgag gcgaaattgc ctgctgcgct acgctcatca
     8821 ggcctactaa gctcctgatt tacaagcctt tgtaggccga accaggcgtt catgccgcat
     8881 ctgacatgaa caaagcgcag gttgtcgcga ttaatcgcgg aagtttttga actggaacgg
     8941 ctgacccaga tcgccaccgc gcaccatcgc catgacagcc tgcaaatcat cacgagattt
     9001 gccagtcacg cggatctcat cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt
     9061 gatcatcttg acgatttttt tctgggtcgc gctctcaatg ccctgtttca gtttcgcttc
     9121 cacaaaccag gttttaccgc tatgaacgat attttccggt acatccagtg aactgccttc
     9181 aatgccgcgc ttcagcagct tggcacgcag aatatccagc aactgattga cctggaaatc
     9241 ggactcgctc aacactttga tggttttgct ggcgtcgttc agctcaaatg aggcttcaac
     9301 gttacggaag tcaaaacggg actccacttc gcggctcgcg ttatcgaccg cgttacgtgc
     9361 ttcctgaaga tcaacttcag agacaatatc gaaagatggc atcttttcct ctcccttcat
     9421 ttttgatgcg aagcataata cccgcaaagt taaataaccg acagtcctgc accaggacgt
     9481 atcccttaat tgtgcatagt cgcaacagca tgcacaatta agggatagcc ttattgaaca
     9541 cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac cgtattggga tgcggtgcct
     9601 tagggcaatt atggcttaca gcactttgca aacagggtca tgaagttcag ggctggctgc
     9661 gcgtaccgca accttattgt agtgtgaatc tggttgagac agatggttcg atatttaacg
     9721 aatcgctgac cgccaacgat cccgattttc tcgccaccag cgatctgctc ctggtgacgc
     9781 tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc gtccacactg cctgtaacca
     9841 cgccaatact gttaattcac aacggcatgg gcaccatcga agagttgcaa aatattcagc
     9901 agccattact gatgggcacc accacccatg ccgcccgccg cgacggcaat gtcattattc
     9961 atgtggcaaa cggtatcacg catattggtc cggcacggca acaggatggc gattacagtt
    10021 atctggcgga tattttacaa accgtgttgc ctgacgttgc ctggcataac aatattcgcg
    10081 ccgagctgtg gcgcaagctg gcagtcaacg gtgtgattaa tccactgacc gccatctgga
    10141 attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat tatgcagata tgcgaagaag
    10201 tcgcggcagt gatcgagcgc gaagggcatc atacttcagc agaagatttg cgtgattacg
    10261 tgatgcaggt gattgatgcc acagcggaaa atatctcgtc gatgttgcag gatatccgcg
    10321 cgctgcgcca caccgaaatc gactatatca atggttttct cttacgccgc gcccgcgcgc
    10381 atgggattgc cgtaccggaa aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg
    10441 aatatgagcg catcggcact ggtttgcctc gcccctggta gtgaagagac tgaagccgtc
    10501 accactatcg ctctgctggt tcgcggcggt atcaaagtca ccactgccag cgtcgccagc
    10561 gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg
    10621 gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc ctggtggcat taaaggcgca
    10681 gagtgttttc gcgatagtac cctgctggtt gaaaccgtta aacagttcca ccgttccggg
    10741 cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc tggtaccgca cgatatcttc
    10801 ccgattggca atatgaccgg cttcccgacg ctgaaagaca aaattcccgc cgaacaatgg
    10861 caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt
    10921 acggctattg attttggtct gaaaattatc gacctgctgg ttgggcgcga gaaagcccat
    10981 gaagtggcat cacaactggt gatggcggca gggatttata attattacga gtagtgtcgg
    11041 atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa ccacaaatta tgggcgatac
    11101 accttcacat tgttaaagcc ctgctcgcgc agatagagcg cctgcagacg gctcatcacc
    11161 ccgcgctcac accacagtag ccaggttctg ttctggtcga gatcgccaaa tttggtgctc
    11221 agtttataga acggcagaga aaccacgtcg ataccttcaa ccttcagcgg cttatcttcc
    11281 tgctcatcga cagaacggat atcgaggatc acgtcgttcg ggccaaagcc attgacggtt
    11341 tccacttcca ccacttcctg ctcggtctgc tgggcgattt cgcggatatc aacgttattc
    11401 gcttcctcaa ccactttatc gagaatactg aagtcgaact tctcttcttc cgcttcaatc
    11461 ttcgatttaa ccgctttcac cgtcgggctt ttggagatca cgccacaata ttctggcatc
    11521 gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt tgatgatgtg ctctttgtcg
    11581 taagaaatca gcggacgcag gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg
    11641 gtcagcgtct gactggacac ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg
    11701 taacgttcag ccactttaga cgcggcacgc accatcatac gtttgaggat aacgcccatc
    11761 tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg gttcgaaatt aatagcgaca
    11821 aaacgcacgc ggtgggagct gccaaaacgg ttccacaaat aatgcgccac ctgacgcacg
    11881 ccaatttcat gcgccgcgcc gccaaggtta aagaaacagt aatgcacgcg gcagccgcga
    11941 cgcatcaaca tataactgga aacaccggag tcgaaaccac cggaaatgag cgacagcaca
    12001 tcttcctggg tgccgatcgg gaaaccgcca ataccttcgt agcggccttt aatcagcagg
    12061 agacgatcgt cttccacttc cagatggaca gtcacttccg gattggtcag cttcacgcgc
    12121 gcggactcaa tatgctgatt taaaccgccg ccgacgtaac gttccacatc aatcgagcta
    12181 aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg ttttgccttc cagctgatcg
    12241 cgatactgaa ccaacgcttt ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg
    12301 acttcgagaa tatggtggat acccggaata cgggtcaggg cgtcgcgaat agccagacgc
    12361 tggttttcat cttttgcgcg aacttcgatg ttatcccagt ggcggacgac agcgagcgtc
    12421 tcatcatagt gctttaaaac gttacgaatg ttcccggtaa ggatttttat aaagcgcaag
    12481 cgcacagatt ggcttttgat ggtgatttcc gggaacaatt taatgataaa cttcatggcg
    12541 gcaatggttc gttggcaagc cttaagcgac ttgtataggg aaaaatacag cagcccacac
    12601 ctgcggctgc atccaggcgc ggaagtatac cactaacatc gctttgctgc gcacatcacc
    12661 ttaccattgc gcgttatttg ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat
    12721 attgagagtc agacattcat tatgccgaag aaaaatgagg ctcccgccag ctttgaaaag
    12781 gcgctgagcg agctggaaca gattgtaacc cgtctggaaa gtggcgacct gccgctggaa
    12841 gaggcgctga acgagttcga acgcggcgtg cagctggcac gtcaggggca ggccaaatta
    12901 caacaagccg aacagcgcgt acaaattctg ctgtctgaca atgaagacgc ctctctaacc
    12961 ccttttacac cggacaatga gtaatggact ttccgcagca actcgaagcc tgcgttaagc
    13021 aggccaacca ggcgctgagc cgttttatcg ccccactgcc ctttcagaac actcccgtgg
    13081 tcgaaaccat gcagtatggc gcattattag gtggtaagcg tctgcgaccc ttcctggttt
    13141 atgccaccgg tcatatgttt ggcgttagca caaacacgct ggacgcaccc gctgccgccg
    13201 tagagtgtat ccacgcttac tcattaattc atgatgattt accggcgatg gatgatgacg
    13261 atctgcgtcg cggtttgccg acctgccatg tgaagtttgg cgaagcaaac gcgattctcg
    13321 ctggcgacgc tttacaaacg ctggcgttct cgattttaag cgatgctgat atgccggaag
    13381 tgtcggatcg cgacagaatt tcgatgattt ctgaactggc gagcgccagc ggtattgccg
    13441 gaatgtgcgg tggtcaggca ttagatttag acgcggaagg caaacacgta cctctggacg
    13501 cgcttgagcg tattcatcgt cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg
    13561 gtgcattaag cgccggagat aaagggcgtc gtgctctgcc agtactcgac aagtacgcag
    13621 agagcatcgg ccttgccttc caggttcagg atgacatcct ggatgtggta ggagatactg
    13681 caacgttggg aaaacgccag ggtgccgacc agcaacttgg taaaagtacc taccctgcac
    13741 ttctgggtct tgagcaagcc cggaagaaag cccgggatct gatcgacgat gcccgtcagt
    13801 cgctgaaaca actggctgaa cagtcactcg atacctcggc actggaagcg ctagcggact
    13861 acatcatcca gcgtaataaa taaacaataa gtattaatag gcccctgatg agttttgata
    13921 ttgccaaata cccgaccctg gcactggtcg actccaccca ggagttacga ctgttgccga
    13981 aagagagttt accgaaactc tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc
    14041 gttccagcgg gcacttcgcc tccgggctgg gcacggtcga actgaccgtg gcgctgcact
    14101 atgtctacaa caccccgttt gaccaattga tttgggatgt ggggcatcag gcttatccgc
    14161 ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat ccgtcagaaa ggcggcctgc
    14221 acccgttccc gtggcgcggc gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa
    14281 cctccatcag tgccggaatt ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc
    14341 gtaccgtctg tgtaatcggc gatggcgcga ttaccgcagg catggcgttt gaagcgatga
    14401 atcacgcggg cgatatccgt cctgatatgc tggtggtcct caacgacaac gaaatgtcga
    14461 tttccgaaaa tgtcggcgcg ctcaacaacc atctggcgca gctgctttcc ggtaagcttt
    14521 actcttcact gcgcgaaggc gggaaaaaag ttttctctgg cgtgccaccc attaaagagc
    14581 tgctcaaacg taccgaagaa catattaaag gcatggtagt ccctggcacg ttgtttgaag
    14641 agctgggctt taactacatc ggcccggttg acggtcacga tgtgctgggg cttatcacca
    14701 cgctgaagaa catgcgtgac ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag
    14761 gtcgtggtta tgaaccggca gaaaaagacc caatcacttt ccacgccgtg cctaaatttg
    14821 atccctccag cggttgtttg ccgaaaagta gcggcggttt accgagctat tcaaaaatct
    14881 ttggcgactg gttgtgcgaa acggcggcga aagataacaa gctgatggcg attactccgg
    14941 cgatgcgtga aggttccggc atggtcgagt tttctcgtaa attcccggat cgctacttcg
    15001 acgtggcaat tgccgagcaa cacgcagtga cctttgccgc cggtctggcg attggtgggt
    15061 acaaacccat tgtcgcgatc tactccactt tcctgcaacg cgcctatgat caggtgctgc
    15121 atgacgtggc aattcaaaag cttccggtcc tgttcgccat cgaccgcgcg ggcattgttg
    15181 gtgctgacgg tcaaacccat cagggtgctt ttgatctctc ttacctgcgc tgcataccgg
    15241 aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg ccagatgctc tataccggct
    15301 atcactataa cgatggcccg tcagcggtgc gctacccgcg tggcaacgcg gtcggcgtgg
    15361 aactgacgcc gctggaaaaa ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga
    15421 aactggcgat ccttaacttt ggtacgctga tgccagaagc ggcgaaagtc gccgaatcgc
    15481 tgaacgccac gctggtcgat atgcgttttg tgaaaccgct tgatgaagcg ttaattctgg
    15541 aaatggccgc cagtcatgaa gcactggtta ccgtagaaga aaacgccatt atgggcggtg
    15601 caggcagcgg agtgaacgaa gtgctaatgg cccatcgtaa accagtcccc gtgctgaaca
    15661 ttggcctgcc tgacttcttt attccgcaag gaacgcagga agaaatgcgc gccgaactcg
    15721 gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg gctggcataa tccctactcc
    15781 actcctgcta tgcttaagaa attattcatg gcaggagtgg cagcatgcaa tacaacccct
    15841 taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc
    15901 cagatcgtgg taatcacgca tggacactgc cggaagagag cagccgtccc ataattaaac
    15961 gcgcactgga aggcggcata aatttctttg ataccgccaa tagctattcc gatggtagca
    16021 gcgaagagat cgttggtcgc gcactgcggg atttcgcccg tcgtgaagac gtggtcgttg
    16081 ccaccaaagt gttccatcgc gttggtgatt taccggaagg attatcccgt gcacaaattt
    16141 tgcgctctat cgacgacagc ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa
    16201 ttcatcgctg ggattacaac acgccgatcg aagagacgct ggaagctctg aacgacgtgg
    16261 taaaagccgg gaaagcgcgt tatatcggcg cgtcatcaat gcacgtttcg cagtttgctc
    16321 aggcactgga actccaaaaa cagcacggct gggcgcagtt tgtcagtatg caggatcact
    16381 acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc actgtgttat caggagggcg
    16441 tggcggtgat tccgtggagc ccgctggcgc gggggcgact gacgcgtccg tggggagaaa
    16501 ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa
    16561 atgacgcgca gatcgcagag cggttaacgg gcgtcagtga agaactcggt gcaacacgag
    16621 cacaagttgc gctggcctgg ttgttgagta aaccgggcat tgccgcaccg attatcggta
    16681 catcgcggga agaacagctt gatgagctgc tgaacgcggt ggatatcact ttgaagccgg
    16741 aacagattgc cgaactggaa acgccgtata aaccgcatcc ggtagtagga tttaaataag
    16801 agtccaggcc tgatgagacg caacaagcgt cacatcaggc atcggtgcac aactacgaca
    16861 gaatgcccag cggccagtga tgaccaataa agtacaggat gccagcggag atcaccccgg
    16921 cgacaatatc atctatcatg atccccatgc cgccatgcac attgcgatca aaccagcgga
    16981 tcggccacgg tttccacata tccagaatac ggaaaatcac aaacccggcg gcaacccact
    17041 gccagtcatt ggtcggcagc gccatgagcg tgatccacat accaataaat tcgtcccaga
    17101 caatgctgcc gtgatcgtgc acgcccatat cttttgcggt ctgatgacaa agatagacgc
    17161 cgatacagat ccccagcatc accaccagcg agtagagctg ccagggcaaa aaggtcatca
    17221 gataccagaa cggaatcgct gccagcgagc ccatcgtccc aggaacgatc gggcttaatc
    17281 cacttccgaa tccgacagca agtagatgcc acggattact catcttcagg cgacttttcg
    17341 cgacatcttt atggcgtggc aaaatggtca tatcctttcc agtctaacgt aacaggttcg
    17401 ccgtcacgaa taaaacaaag cccttcgata tcggcggtca tttgcccgat acaggtaaac
    17461 ggtacgccca ggtgtccgag agccacatcc agcgctccac ggttcagttc cggcaccgtg
    17521 aaacacaact cgtaatcttc accgccagag agcgcccagc gcagcgcctg ttccggttca
    17581 acatggcgag aaagcgcatc agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag
    17641 tcgctggctt tcacgatatg cccgagatcg gaaatcagac cgtcagagag atcgatagct
    17701 gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac gcggcgatgg acggagatga
    17761 cgtttgatca agtagtcagc atctttagca tcggcaacct gcaaacggtt ttgcaaaatc
    17821 gccagcccgg cggcgctatc gcccggtgta ccggtcacat agatccagtc acccggtttc
    17881 gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt
    17941 gataatggcc cacgcgtggt atcgccgcca atgagttgca tatcgtaata attgagaaga
    18001 tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt ctacgtccgg taaggttaat
    18061 gccagcgtca gccaggccgg atcggccccc atcgctgcca gatcgcttag gttcaccgcc
    18121 agtgctttat aagccagatc agcaggatcg atatcaggga ggaaatggtt acccgccacc
    18181 agcgtatcag tgctgatcgc cagggtctgt ttctcgggga tattgagaag tgcgcaatcg
    18241 tcgccgatgc ccagttcgac atcaagacga gaacttctta cacggtcaaa ataacgggca
    18301 atcagggaga actcgccaca tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc
    18361 ggcgatcatg gaacggtctt ccgtgaatct accggcctgg atatcacttt ttgttagggc
    18421 gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc
    18481 cgaacgattt cgccagttcg atcgcttcgt taatggccac tttgtatggc acatcgctac
    18541 gtttagacag ttcgtacagc gcaatgcgca gtactgcttt ttctacctgt cccagttctt
    18601 ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag gtatgcggta ttagtcgcca
    18661 ccccggccag cagctcacgg aagtacagga cgtcaacgtc ttttacatcc tgttcagcca
    18721 ggaactggta ttcaacatca gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct
    18781 ggacggcaca ctcacgagcg cggcgacgag cagcaggttt cacggatttc cccttactaa
    18841 tttcaggcct tgatggcttt caatacatta atcatttcaa gcgcggtcag tgcagcttct
    18901 gcacctttgt tgccagcttt ggtgccagca cgttcgatcg cttgttcaat gctttcagtg
    18961 gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg caacatgcgc caggccgttg
    19021 cttgcaccac cagcgacata ttcaaagtgg gcagtgccac cacgaataac cgtacccagc
    19081 gcaatcaccg cgtcgtattt accggtttta gccagtgcac ccgccgccag cggcagctca
    19141 taggcaccag gcacccaaac aacggtaatg ttttcatctt ttacctgacc gatacgtttc
    19201 agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa agttgttgaa acgcgcgatg
    19261 gtgatggcga cgcgagcgtc cggggtagca acgttagctt caataatgtt cattttcttc
    19321 cttcgggttc gagtatggcc ccgcaggggg gcggatttta gcataatatt tcgtgcgctg
    19381 cttccctttc gagccgggag atcatgcacc cactaaatgc aggcaaacat ccgggcctac
    19441 atgacgtatc tctttgaatt taaattgggg ggcgtcggct aatttctcaa gccctggcag
    19501 cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt gcgatataga caatcagctc
    19561 atcgactaaa cccgcctgca gcaatgcacc agcgagcgtt ggccccgctt ccacccagat
    19621 gctgttaatt tgctgtttac ccagttgcat catcagtaca accagatcca gatgaccttt
    19681 atgctctgga atcagcaagg tacgcaccgt ttccggccac tcacgagaat cttcctgcgt
    19741 acgcgcgaac caggtttcgc cgggctgctg cacaatgtga tgttccggcg tcacgcgatt
    19801 ttggctatca atcacaatac gtatcggctg gcggagattt tgttgcgggt agagcgcctg
    19861 agtttgttca tccagttcgg accaacgcac cgttaaggca ggatcatccg ccagcaccgt
    19921 ggcgctgctg gttaaaatgg catgactttg cgcgcgcagt agttgtacat cacgccgcgc
    19981 ctggggcgaa gtgatccact ggctttcgcc gctcgccatc gccgtgcgac catcaagcga
    20041 tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc atccgcttga gaaagccttt
    20101 attcaattgc tcggcttcac tcatcatcag gccgtggctg acgtcaatgc cagcctgttg
    20161 cagacggtaa agtccacgcc cagcgacctg cgggttagga tcttgcatcg aggcaaccac
    20221 gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt ggcgtacgac catgatggct
    20281 acagggttcg agtgtgacat aggcgatcgc acctttggct ttttcacccg ccatacgcaa
    20341 cgcgtgtact tcggcatgtg gttcacccgc acgttggtgg taaccttcac cgacaatttc
    20401 gccatctttg acaatgacgc acccgacatt cgggttggga tgcgtggtaa aacgtcctcg
    20461 ttgcgccagc tttagcgccc gcgccatgta atactcgtcc tgcacggctt agtcctccag
    20521 gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa ctgcggtaga cagaggcaaa
    20581 acggatatag gcgactttat cgagcttttt caattgctcc atcaccagat tgccaatcat
    20641 cttgctcggc acttcgcgct caccggtggc gcgcagctgc gatttaatat gattgattgc
    20701 catttcgacg tcatcggaac tcaccggacg tttttccagc gcccgcagca ttccgctacg
    20761 caatttctct tcattaaacg gttcacgcac gtcgttgctt tttacaacac gcggcataac
    20821 cagctccgcc acttcaaagg tggtgaaacg ttcattacac accagacact gccgacggcg
    20881 gcgtacggat gacccctcgc ccacgagacg agagtcaatt accttagtgt ccacggcgaa
    20941 acagaatggg caatgcatac ggcgtcctga ccaggtggtt aacagaaatc tattttaccc
    21001 tgaactgacg atacaacaaa ggcgcaacgc ttttgagata aaggatcgat gctttcgctg
    21061 cttttaccct ctaccattag gctaattgcg acgagaaagg gacgacctat gaatactaac
    21121 gtttttcggc tgctcctgct gggaagcctg ttcagcctta gcgcctgtgt gcagcagagc
    21181 gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc aagagatgac gcaactcaat
    21241 aaagaaacgg tcaaaatcac tcagcaaaac aggctgaatg caaaatccag cagtggggtt
    21301 taccttctgc caggagcgaa aacaccggca agactggaaa gccagatcgg tactttacgt
    21361 atgtcgctgg tgaatattac gcctgatgca gatggcacca ccctgacgct acgtattcag
    21421 ggcgagtcga atgatccctt gcccgcattc agcggaactg ttgaatatgg gcaaattcag
    21481 ggaacaatag acaactttca ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc
    21541 agcgtcctcg cccccagcga tgttgatatt ccgttacagt taaagggtat ttctgtagaa
    21601 cagttagact tcgtacgtat ccacgatatt caacctgtta tgcagtaaac gtatttcggg
    21661 gcgattttgt gcgtcccgca acatctttcc ccgtcatttt gttactccgc ttacatcacc
    21721 cagattgata gtaaaagttt gcaacaaggg tgaaagtcag tacaatccct gcccgaatgt
    21781 gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac tgtgaaacga aacatatttt
    21841 tgtgagcaat gatttttata ataggctcct ctgtatacga aatatttaga aacgcaattt
    21901 gcgccttttt cactcccgca aaggattttc aaacagtggc atacatatga aaaaaacatt
    21961 actggcagcc ggtgcggtac tggcgctctc ttcgtctttt actgtcaacg cagctgaaaa
    22021 cgacaaaccg cagtatcttt ccgactggtg gcaccagagc gttaacgttg tcggaagcta
    22081 tcacacccgt ttcggaccgc agatccgcaa cgatacctac cttgagtacg aagcattcgc
    22141 taaaaaagac tggttcgact tctatggtta tgcggatgcg ccggtattct tcggcggtaa
    22201 ctccgatgca aaaggtatct ggaaccacgg ttctccgctg tttatggaaa tcgaaccacg
    22261 tttctccatc gacaagctga ccaatactga ccttagcttc ggtccgttca aagagtggta
    22321 cttcgcgaac aactacattt acgacatggg tcgtaataaa gatggtcgcc agagcacctg
    22381 gtacatgggt ctgggtaccg atatcgacac tggcctgccg atgagcctgt ccatgaacgt
    22441 ctatgcgaaa taccagtggc agaactatgg cgcagcgaac gaaaacgagt gggacggtta
    22501 ccgtttcaaa attaaatact ttgtgccgat taccgatctg tggggcggtc agctgagcta
    22561 catcggcttc accaacttcg actggggttc cgatttaggg gatgacagcg gtaacgcaat
    22621 caacggtatt aagacccgta ccaataactc tatcgcttcc agccatattc tggctctgaa
    22681 ctacgatcac tggcactact ctgtcgtagc tcgttactgg cacgacggtg gtcagtggaa
    22741 cgacgatgca gaactgaact tcggcaacgg caacttcaac gttcgctcta ccggctgggg
    22801 tggttacctg gtagtaggtt acaacttctg attatgaaaa tgccgggatt tattcccggc
    22861 atttcagatt gttaacaacg tgcgctttgt tcatgccgga tgcggcgtaa acgctttgtc
    22921 cgacccacaa aagcacgtaa attcaatata ttgctgaaat aacgtaggcc tgataagcgt
    22981 agcgcatcag gcaattttac gttttaatca cttcttcttg ttcatcatcg ctttcaggtc
    23041 agcgaatggg ttgtacttcg cttcaccgac atctttctgc gcatcttccc ctgcgataac
    23101 ggtcgtacca tactgatccg cttcggtata tttcgaatgc tcatgatcgt ggcaatagag
    23161 acaaagcaat tcccagttac tgccatcttc cgggttattg gtatggtcgt gatcaatgtg
    23221 gtgaacagta agttcacgca ggttggaata aacaaactcg cgggaacagc gaccgcagac
    23281 ccacggatat atttttaatg ctttttcgcg atagccgctt tctaaccgcg catagttttt
    23341 tgggatgata gccatgaaga gttacctgga tgaaatgtag ggttacgcgc agtttcccaa
    23401 gatgcgggga ttttcgcaat ctccatacat caaaaaaaat cccgatcttc tgaccgggat
    23461 tttcaacatc aacgggaact tgattacggc agaattgacg gctgatccgc cccttctttt
    23521 tccactttct gctgcaacat gtgttcgcgc ttcataccca gtttcagagc caacgcagat
    23581 gccacataga tggaagatgc agtaccgatg gaaacaccga taagcatggt cagcgagaag
    23641 ccttccagta ccggaccacc gaagaggtac agcatcagga taaccatcaa ggtagtaccg
    23701 gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca cgttaaagat ttcgtaaggc
    23761 gtaccgcgac ggatcttgcg gaagttttca cgaatacggt ccgataccac gatactgtcg
    23821 ttaagcgagt aaccgataac cgacatcaac gatgccacaa tggtcaggtc aatctcgata
    23881 tggaataacg acaaaatacc cagcgtaatg ataacgtcgt gcgccagcgc aataacaacc
    23941 cctgccgcca gtcgccactc aaagcgaaaa cctacgtaca caaggataga cagcagcgcc
    24001 gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc ccacgctcgg accgacgaac
    24061 tcaatacgct tcactgctgc attctgattg gtggattcgt taatcacctt cagaacctgg
    24121 ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg gcatacggac catgatgtcg
    24181 tggctgctac caaagttttg cagcattggc tcttcaaaac cggctttttg caatgcatca
    24241 cgcattacgt caatttcagc cggtttttcg agcgtaattt cgataaccgt accaccggtg
    24301 aaatccagcc cccagttaaa gccgcgcacg cccataataa cgatagcggc gattaacagc
    24361 agaccagaga tgccgaaagc ccagtagtcc cagcgcataa agtcatagac tttacggccg
    24421 tggtttagtt gttcaacagt atattcctgt gccacatcgc actcctcaga ttgacagctt
    24481 cttgacgcgc ttgccgccat atagcaggtt tacgatggca cgcgtaccga caatcgcggt
    24541 aaacatcgac gtcgccacac cgataccggt agtaatcgcg aaccctttaa ttgccccggt
    24601 acccactgcg tacaggatga tgactttaat cagcgtggtg atgttcgcat cgaagataga
    24661 actgaatgcg ccacgataac cttcatcaat tgcctgttga acagtacgtc cgttgctcaa
    24721 ctcttcttta atacgttcgt tgatcagtac gttcgcatcg accgccaccg caagggttaa
    24781 gacgataccc gcgatacctg gcatactcag cgttgcgcct ggcaacagcg acataatgcc
    24841 gacgattaag atcaagttgg caatcagagc actggtcgca atcagaccaa acttcttata
    24901 gaagatgatc atgaacagaa tagaaaccag cagaccggca aggcaagcct ccagcccctg
    24961 ttcaatgttc tgcataccca gggttgggcc aatggtacgt tcttcaacaa tctgaatcgg
    25021 cgcgatcaac gcaccggcac gcagcagcag tgacagctga cgggcttcgt tcgggttgtt
    25081 gatgccggtg atacggaagc tgttacccag acgagactgg atgttggcga tgttaatcac
    25141 ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct ttcttaccgc tgtctttgta
    25201 ctccacaaac agggttgcca tcggtttgcc gatgttgtcc ttagtgaagt tagacatgat
    25261 gttaccacca gcgctatcga gcgagatgtt aacctgcggt tggttgtatt cgtcctggct
    25321 ggaagtggag tcggtgatat ggtcaccggt cagaattacg cgtttgtaca gcacaactgg
    25381 ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt acgcgaccgg atgccgcagc
    25441 ggcctggtca acgttggtgt ttaccagacg gaattccagc gttgccgtcg cacccagaat
    25501 ctctttcgca cgcgcagtgt cctgaatacc tggcagttca acaacgatac ggtcagcacc
    25561 ctgacgctga accaccggct ccgccacgcc aagttggttt acacggttac gcaggatatt
    25621 gatgttctgc tgcaccgcat attcacgcgc ttcactcaga cgagcatcgc tcattaccgc
    25681 acgcagctgg ttgctgccct ggctactaat caccaggtcc ggatggcgct tgctcagata
    25741 cgcaatggct tcatcacgag ctttagcatc gcggaaagta atgctcaggc cgtagttgtt
    25801 ttctttacga acggtggtat acgggatgcc cttttcacgc agatcgctgc gcaggctatc
    25861 gatattttgt tcctggagtt tgccaagcgc ggtgtccata tccacttcca tcaggaagtg
    25921 aacgccgcca cgcaggtcaa ggccgagctt catcggctca gcgtgaatag ctgccagcca
    25981 gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat ttgtcaccca taacgcccat
    26041 taatgcttca cgagcgcgca gctgggtgtc agtggagtcg aagcgcgcaa gaatagcgcc
    26101 ctcttccagt gccacagact tagcagttat tttttcttct tgtaacgttt tctggacctg
    26161 gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca gtgatctgaa cagccggatc
    26221 ctcaccaaac aggttgggaa gcgcatacag cagaccgatg acaatcacca cgatcagcat
    26281 gacgtacttc cacaaaggat aacggtttaa cacggcaatt cccttaggga aaaattttaa
    26341 ttacagcgcc ttcatggtgc ctttcggcag gacggcagct acgaagtcac gtttaataac
    26401 tacttcagtg gtgtcattca gcgcgatagc aatgtagccg ttttccgcta ctttggttac
    26461 gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct ttggcaatgg agtccatcag
    26521 ctttttgtgt tctttggtgc gcttctgctg tggacgcagg atcatgaaat agaaaatcag
    26581 accgaatacc accagcatca aaatcaaaga catcgggcta ccttgcgccg gtgcacccgt
    26641 tgccgctacc gcatcagaaa taaaaaagct cattaaattt ccctcattat taatattaat
    26701 caacgttcaa aggtggtact tctcgcccct gacgctggta aaaatcagtt acgaagctct
    26761 ctaatttacc ctcttcaata gccttgcgta aacccgccat caaacgctgg tagtaacgaa
    26821 ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt gcaacggtca agatgatgca
    26881 agtaagcgcg tgaataattg cgacaggtgt agcaatcaca ctcaggatcg agtgggccag
    26941 tatcgctctt atacttcgca ttgcggattt tcaccacgcc atcggtcacg aacaaatgac
    27001 catttcgggc gttgcgggtt ggcattacgc agtcaaacat atcgatacca cgacgtacgc
    27061 cttcaaccag gtcttctggt ttaccaacgc ccatcaggta acgcggtttg tctgccggaa
    27121 tttgcgggca tacatgctcc agaatgcggt gcatatctgc tttcggctca cccacagcca
    27181 gaccgccgac agcgtagcca tcaaaaccga tatctaccag acctttaaca gaaatatcac
    27241 gtaaatcttc gtaaacgctg ccctgaatga taccaaacag cgcatttttg tttccgagac
    27301 tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga catctccatg gagcgttttg
    27361 cgtaatccca gtcagcagga tacggcgtac actcatcaaa gatcatgacg atatccgaac
    27421 caagatcgta ctgaatctcc attgattttt caggatcgag gaaaatcgga tcgccgttga
    27481 tcgggttacg gaagtgcacg ccctgttcgg tgattttacg aatatcgcca aggctgaaga
    27541 cctggaagcc gccggagtcg gtgaggatgg gccccttcca ctgcataaaa tcgtgcagat
    27601 cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca caggtggaag gtgttgccga
    27661 ggataatttg cgcgccagtg gcttcaactt cttccggcgt catccctttt acggtgccgt
    27721 aggtgccaac aggcataaaa caaggcgttt ccactacgcc acgatcaaag accaggcggc
    27781 cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt cattttttct ccactacgtc
    27841 agaaaaacag tccaacgttt taaaccagcg ccgcggaatt actccccgac gcgctcatta
    27901 attgcctgcg gattgtacgt gataaacatc gcatcaccgt aactaaaaaa gcgatatttc
    27961 tcttctaccg ctgctttata ggcgttcatg gtgtgttgat aaccggcaaa ggccgaaacc
    28021 agcataatca gcgtcgattc cggcaggtgg aagtttgtca ccagcgcatc gaccactttg
    28081 tactggaagc ccggatagat aaagatttgg gtatcgtcaa agaacggttc gataagatcg
    28141 tttttcgccg cctgagccgc gctttccagc gaacgtactg aggtggtgcc aaccgcaatc
    28201 acccggttac cgcgcgcttt cgccgccagt accgcgtcta ccacatcctg cggcacttca
    28261 gcgtattccg agtgcatgat gtgatcttca atggtgtcga cgcgcaccgg ctggaaggtg
    28321 cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc ctttggcgcg taatttttcc
    28381 agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg caaccgcgcc cggtttttcg
    28441 ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt ccggacggtc gatatacggc
    28501 ggcagcggca tatggccgat gctgttgaga atatccagca ccgagcgttc atcattaaat
    28561 tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg cgttaatact ttcgtcatca
    28621 cccagcagca gttctgcgcc aggttttggc gctttcgagg cgcgaatatg cgcaagaatg
    28681 cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct tgccgccgct ggctttacgc
    28741 ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa ccagaagatc gccggggttg
    28801 agcttatcga ataaatcggt gaaagtaccg tgcgtcagcg cgcccgtcgg cccgtccagc
    28861 gacagtaaac gacagctact gcgttcaggc atgggatagt gggcaatcag ggattcgggc
    28921 aattcaaagg agaaatcggt aacgcgcatg acactgactc agactaaaat aagaggcggg
    28981 tagtctagtg ccggggcgct ctccctgcaa cctttacccc cttttggata gaatataatc
    29041 gatgaatttt ttagctcacc tgcatttagc ccatctcgcg gaaagctcgc tttccggcaa
    29101 tttactggct gatttcgtac gcggaaatcc tgaagaaagt tttccgcccg acgtcgtggc
    29161 tggcattcat atgcatcgac gtatcgacgt attaaccgac aatctgccgg aagtccgcga
    29221 agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg cctattacgc tggatgtcat
    29281 gtgggatcac tttctttccc gccactggtc gcagctgtcg ccggactttc cgctacagga
    29341 atttgtctgt tatgcccgcg agcaagtgat gacgattttg ccggactcac cgccacgttt
    29401 tatcaatctg aacaattact tgtggtcaga gcagtggctg gtgcgctatc gcgatatgga
    29461 tttcatccag aacgtgttaa acggcatggc aagccgccgc ccacgtctgg atgccctgcg
    29521 tgactcctgg tacgatttag acgctcatta tgacgccctc gaaacccgct tctggcagtt
    29581 ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta taaaacgtta gttcatccat
    29641 accgtagccg aaatggcagg caaagccaga atcccgtcag tcagttgccc atgcccttct
    29701 ttgcattgcc attgcacggc attgagcaac ggtgacgcgg gtagcacgac ttcacaagct
    29761 tcgccacggt tgattgccac cagtacacgc tgctgattca gcacgcggac aaataccacc
    29821 acgttatctt ccgcatacag cacctgacag ccgccatgac gtagcgcctg acttttctta
    29881 cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg tatcctgctt ttccacctgc
    29941 caggggaacg gtttacggca aaacggatcg tttttgccat ccagtcctac ttcatcaccg
    30001 taataaatgc acggtacacc aggccaggtg aacagccaga ccaccgccag cggcaggcgc
    30061 gcaatatccc gaccgagcag cgttttaaat cgcgcagtat cgtgactgtc gagctgatta
    30121 aacatacgta attgttgttg atgagaaagc cctgcgcggt aattatccat ccaggccata
    30181 caggtttggg catcaatttg ctgcggatcg taagagatat cggtattggc aagaaatccc
    30241 cacaacggga atgtgaagcc acgatagttc atggcggcat cttccacatc ggcctgtaac
    30301 cattgccgtg catcgccaaa atgttcgccg acaatatacg cttccggctg ggtttctttc
    30361 gccgcttcgg tgatcccagc aacgtgctgc atattattgc gcgccccacc cgcctcccct
    30421 agcatatgca ccacatccag ccgccagccg tccatactcc acggcgcttt cagccaatgg
    30481 cggacaatac tgtcttcccc gcgataaatt tcattcacca gactttccga ctgataatcc
    30541 agcttcggca agctggcata gccaagccag tcgagcgccg tgccatcatc actaaacgag
    30601 taccagtcgc gccagggcga ttcagggttg tgacaagctc cccccgtgcc acgattgtgc
    30661 ctgtcaaacc aggcatggga atcgccactg tggttaaaca cgccatccag caccagccgc
    30721 attcccagct gctgcgtatt gtggcgtaag cgcagcaacg ccccatcacc gccaaactgc
    30781 ggatcgacat ggcgataatc ctcggtatcg tatttatgta cgctgggagc tttaaacacc
    30841 ggattgagat acagcgccgt cacgccaagc tttttcagat acggcagttt ttcgcttatc
    30901 ccgtccagat cgccgccata gaacgttgat ccgcccgcct gcgccgtgac cggttcatcc
    30961 cagtcacgca agatgatctc ttgtccggct gcatgatggt aatagacatg atcctgttca
    31021 gcttcacgag gaagactacg cgcaaaacga tcagggaaga tctgataaaa aatctgatcc
    31081 gcagcccatt gtgggccgat atccggtaca tcgacggcaa actgctccag tcgtgccggc
    31141 ggcattcggc tgaagccctg cggtgtaaac caacgctggc gatcgtgcca cagcagtttg
    31201 aaactgtaac gccgccgggg ttgtccgctg gagagatcaa tcgccgcacg ccatgcggtg
    31261 acgccaggct gctgctgact gcgctgctta tgcatcgata ctgacatttc ttcgttatcg
    31321 tgttctgtac gcagcataat gcgctgcggt gggtcttcgc ccgtcagcca cagtgtaatg
    31381 agcagttgat ctttgctttg tttaacaaat gggggcaccg gcaggtgcca tgcatttaac
    31441 atcataattc ccctgtgatg aaaatctgat caccttgcca tagcctaatg caggatcact
    31501 catcatctga aggatatttg ggggaggagg acgggggcgg ataactttcg gaatagccgg
    31561 atcaggattt cctcctgaac cggcttacag aacgcaagga ttactggttt tcagccagct
    31621 gacgatcgtg gcgacgctta aacatccagc caatcaacag cacaacaatc cacgcgaaac
    31681 cgacatacag cgagatacgc gtatccgggt gataaccaat caacccgata ataaagagca
    31741 ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg cactttaaat ttcagcgcct
    31801 taacttcttc tggcggcaaa cggcgacgga aggcgatttg cgacagcagg atcataatcc
    31861 acacccatac cgtggcgaag gttgccagcg aagcgatcac caggaagacg ttttccggca
    31921 tgatgtagtt cagatacacc gcaaacagca gcgcggtagt catcaccagc accgtaaccc
    31981 acggaatacc gcgacgcgac gttttgctga aaattttcgg tgcgctgccc tgctctgcca
    32041 taccgtggag catacggcct acgccaaata cgtcactgtt aattgccgac agcgaagcgg
    32101 tcagcacaac aaagttaaga atgctggcgg caaaggtaat gcccatatgc tggaacgtca
    32161 gcacgaacgg gctaccggca gtgccaacct gattccacgg gtagatagac ataatgacga
    32221 acagcgtacc gacgtagaac accagaatac gcatcggcac ggagttaatc gcacgcggaa
    32281 tcgatttctc aggatctttc gcttcaccgg cggtaatccc gataatttcg atcccaccgt
    32341 aagcaaacat caccatttgc aacgacatta ccatgccaag ccagccgtta ctgaagaagc
    32401 cgccgttgct ccacaggtta tgaataccgg tcggttgccc gccgttgcca atcccccaga
    32461 tgatgatgcc gaaaccggcg acaatcatga tgatgatggt ggcgacttta aagaacgaga
    32521 accagaattc cagctcaccg aataccttca cgctcatcag gtttacggcg caaatgatca
    32581 gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg gaaccagaca cccatataga
    32641 taccaaaagc ggtcacatcg gcgatggcga caattaggat ttcaaagcag taggtccagc
    32701 cggtaatgta acctgccagc gggccgaggt tttcctgcgc ataacgcgag aaagagctgg
    32761 cggccgggtt atgtaccgac atttccccca gcgcacgcat aatgatatac gccgcgatac
    32821 caccgataat ataggccaac aacacgctcg gaccggccat tttgatggcg tctgccgaac
    32881 cgtaaaacag cccggtgcca attgctgaac ccagtgccat aaagcgtatg tggcgggtac
    32941 ttagcccacg ctttagcttg ttcttacttt ccatcaattc ctaacccatc aacacaataa
    33001 aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg ttcagtgatt tagtgagcgc
    33061 tggaggtcac ctgacgacct gccgcgcgat cccagataat ggccagaacc accatcacca
    33121 ctgttggcat taaccacgcc agaccttgtt cggccagcgg taaacgctgc gcccaggacg
    33181 gtaagatatc gctgaatgca gatgctttga tcccgtcgag aataccaaaa agcaggctga
    33241 taaacatcgg cggagcaatc acgcgggacg aattatgcca ccatgagcgt gtaaaactta
    33301 atacaaccag tgcgatacac ggcggataaa tagcggtcag caccggtacg gagatctgga
    33361 tcagctggct taagccgagg ttagaaacca ccatcgagaa gccgccgagg ataaacacca
    33421 gcgtacgata agagagcggt acgtactggg caaagaattc tgcacaagca caggtcaggc
    33481 caactgccgt taccaggcag gcgatgaaga ttaacgccgc cagcaggaag ctaccgccgc
    33541 cgccaaaggt gtgctgaacg taagcatgca gaatagcagc gccgtttgca gactgatcga
    33601 ccagcgacgc gctgtctgaa cccagacgga acagcgccag gtacagcaga gtcagaccaa
    33661 cacccgccat caggccagcc cagacggtat aacgggtcag cagacgcgct tcggtaacgc
    33721 cacgagaacg cgccgcgtta acaataacga taccaaacac cattgcgccc agcgtatcca
    33781 tggtcagata gccgttaacg aagccgttag aaaacgcagc gttttgataa gcctcagtcg
    33841 ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac agacaggatg accagcgcga
    33901 taattttcag cggcgcaagg aagttgccca cggtatccag cagcttgccc ggatagagcg
    33961 aaaccagaat aacgatagcg aaatagacca ggctgtagat aaacagcggc agcgcggaat
    34021 caccagtcag cggcgcaatc cccacttcaa aggaaacggt agctgtacgc ggcgtagcga
    34081 aaagcggccc caccgccagg taacaaaccg ttgccagcag cacgccagcg actttaccga
    34141 ttggcgtgct gaggctgtca acaccgccgc caacttttgc cagcgccact accgttaata
    34201 ccggcaggcc aacggcagta atgaggaagc cgaatgccgc agtccagacg tgttcgcctg
    34261 cctgcaagcc gaccattgga gggaaaataa tgttacctgc gccgacgaac aacgcaaatg
    34321 tcataaagcc cagagcgatg atatcgcgcg atcttaattg atgggtcata aatcatactg
    34381 cctgtggatg tggtgttgta acgttaaatt tttgtgccaa tccctgggga tgatacagcg
    34441 gtatatttaa acaattttag cgggaaatac tcccaaccag gcgcggcgaa gaatagtttt
    34501 tcgatttacc gcgttaatgc agtcagtatg acagcacctg aagcgcaatt taaacgctta
    34561 taacgtttta aagcaaggag ggatctaaaa accagaggta tatagcgata agaaatcact
    34621 aaccagtata aaacactatt tccgtgaaaa agacatggct aatcatgcga acaaaaaatc
    34681 accaaccgtt gtaaaatcta acgcccgtat tttgactgca aagaaaataa gccagcatcg
    34741 actggcttat ggcaataaaa gatgacaaag gcggattaat cgctgttttt ggcaattaaa
    34801 cgttccggga taacaaaact gaaacgtgtt ccttttccta ctgtactctc aatattcagg
    34861 cgactttcgt gatgattcac agcatgtttc acgatcgcta accctaatcc gctaccgccg
    34921 gtttgccggg aacgcgcttt atcaacgcga taaaaacgct cggtcaggcg cggaatatgc
    34981 tccggtgcaa tgcccggtcc gttatcttca acgctaaatt cggcaccgtg cggcactcgc
    35041 tgccagcgta cggtgatatg cgtgccttct ggagtatgat tcacggcgtt atagaccagg
    35101 ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca ctttgaggcc gttatctatc
    35161 tcaaaggtaa atgtctgttt tttctgactc agagtctgag cctcacgctc aacaacgcgc
    35221 agcatcatcg gcacatcaac cttttcattg agcaaatgcg tcggtgcggc ttctattttc
    35281 gacagcgtca gcaactgctt caccagccct tccatccgct gggtctgctc gcgcatggtg
    35341 tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct catccatcat ctccaggtaa
    35401 ccctgtaaca cggtcaatgg cgtgcgtaac tcatggctca cgttggcaaa aaagttacgc
    35461 cgcgcccctt ccagttgatg catttgcgtg acatcacgcg ccaccatcag caactgtttg
    35521 tgggtataag gcatgacgcg aatttccaga tgccgcccgg tgttgagcac cagattgagc
    35581 gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact ccgggtaacg cagtaggtta
    35641 aggatgttct gcccgttatc ttccggccag cgcaaaccaa gaatttgttg cgccagaccg
    35701 ttacaccaga aaataccgcc ctcttccgtg gtcagcacca ccgcgtcggg cagcgactcc
    35761 gcgccgctac gaaagcgttt aatcagattg cccagttcac ggcggcgttt tttatttcgc
    35821 agctgcatct ggtgtaagcc gtatagtagc ggttcccagc taccacgccc cggtggcggg
    35881 gtcatactgc gatccaccca cagccaccat gaaaggcgca ataaattcca gaaatgccag
    35941 ataagcagtc ctgttaccga tgccagcaaa aaccagggca ggtaaccaaa aaatgcaccc
    36001 aggatgaaag ccgggaggca gcaaagtagc agctccagca ccagcctttt ccacgacagc
    36061 cgttccagca cgtaagatac tccagttaag aaatcataag ccctgctctg cgtccgatga
    36121 gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac cgcgcacggt ctgcaccatg
    36181 cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac gaatgtggac atcgaccgtg
    36241 cggtcttcca catagacgtt agttccccag acgtggttta acagctgctc gcggctgtac
    36301 acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa attctgtcgg ccccatctcc
    36361 agcggctctt cgcccgccat cactcggtga gatgtcgggt cgagacttaa tccctgcatc
    36421 tcaatcacct cttccaccgc cattggcgaa atacggcgca ttaccgcttt gattcgcgcc
    36481 accagctcct tcggcgaaaa cggcttggtg atatagtcat ccgcgccggt ttcaaggccg
    36541 cgcacgcgat cttcttcttc ccctctggcg gtcaacatca ccactggaat atcccgggtc
    36601 atcgactcgc gcttgaggtg tttgatgaac tggataccgg agccgccagg taacatccag
    36661 tcaaggagaa ttaaatccgg ccagggttca ttcagttgat tcacagcact gtcataatct
    36721 tccgcttcga ccggctgaaa gccattttgt tcgagcacga agcagaccat ttcgcgaatt
    36781 ggagcttcat cttctacgac cagaatacgt ctcgccatga tttgccctgt tgtaataaat
    36841 aggttgcgat cattaatgcg acgtcattat gcgtcagatt tatgacagat ttatgaaaag
    36901 ctcgtcgcac atatcttcag gttattgatt tccgtggcgc agaaaaaagc aaatggcaca
    36961 tctgtttggg tataatcgcg cccatgcttt ttcgccaggg aaccgttatg cgcatccttc
    37021 acacctcaga ctggcatctc ggccagaact tctacagtaa aagccgtgaa gctgaacatc
    37081 aggcttttct tgactggctg ctggagacag cacaaaccca tcaggtggat gcgattattg
    37141 ttgccggtga tgttttcgat accggctcgc cgcccagtta cgcccgcacg ttatacaacc
    37201 gttttgttgt caatttacag caaactggct gtcatctggt ggtactggca ggaaaccatg
    37261 actcggtcgc cacgctgaat gaatcgcgcg atatcatggc gttcctcaat actaccgtgg
    37321 tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg cgacgggacg ccaggcgcag
    37381 tgctgtgccc cattccgttt ttacgtccgc gtgacattat taccagccag gcggggctta
    37441 acggtattga aaaacagcag catttactgg cagcgattac cgattattac caacaacact
    37501 atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc catcatcgcc acgggacatt
    37561 taacgaccgt gggggccagt aaaagtgacg ccgtgcgtga catttatatt ggcacgctgg
    37621 acgcgtttcc ggcacaaaac tttccaccag ccgactacat cgcgctcggg catattcacc
    37681 gcgcacagat tattggcggc atggaacatg ttcgctattg cggctccccc attccactga
    37741 gttttgatga atgcggtaag agtaaatatg tccatctggt gacattttca aacggcaaat
    37801 tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc catggcagtg ctgaaaggcg
    37861 atctggcgtc gattaccgca cagctggaac agtggcgcga tgtatcgcag gagccacctg
    37921 tctggctgga tatcgaaatc actactgatg agtatctgca tgatattcag cgcaaaatcc
    37981 aggcattaac cgaatcattg cctgtcgaag tattgctggt acgtcggagt cgtgaacagc
    38041 gcgagcgtgt gttagccagc caacagcgtg aaaccctcag cgaactcagc gtcgaagagg
    38101 tgttcaatcg ccgtctggca ctggaagaac tggatgaatc gcagcagcaa cgtctgcagc
    38161 atcttttcac cacgacgttg cataccctcg ccggagaaca cgaagcatga aaattctcag
    38221 cctgcgcctg aaaaacctga actcattaaa aggcgaatgg aagattgatt tcacccgcga
    38281 gccgttcgcc agcaacgggc tgtttgctat taccggccca acaggtgcgg ggaaaaccac
    38341 cctgctggac gccatttgtc tggcgctgta tcacgaaact ccgcgtctct ctaacgtttc
    38401 acaatcgcaa aatgatctca tgacccgcga taccgccgaa tgtctggcgg aggtggagtt
    38461 tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag aatcgggcgc gtaaccaacc
    38521 cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc tgcgccgacg gcaaaattct
    38581 cgccgacaaa gtgaaagata agctggaact gacagcgacg ttaaccgggc tggattacgg
    38641 gcgcttcacc cgttcgatgc tgctttcgca ggggcaattt gctgccttcc tgaatgccaa
    38701 acccaaagaa cgcgcggaat tgctcgagga gttaaccggc actgaaatct acgggcaaat
    38761 ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca gagctggaga agctgcaagc
    38821 gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg caatcgctga cagcgagttt
    38881 gcaggtactt actgacgaag aaaaacagtt aattaccgcg cagcagcaag aacaacaatc
    38941 gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa gccagccgcc gtcagcaggc
    39001 cttgcaacag gcgttagccg aagaagaaaa agcgcaacct caactggcgg cgcttagtct
    39061 ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc atcgcagaac acagcgcggc
    39121 gctggcgcat attcgccagc agattgaaga agtaaatact cgcttacaga gcacaatggc
    39181 gcttcgcgcg agcattcgcc accacgcggc gaagcagtca gcagaattac agcagcagca
    39241 acaaagcctg aatacctggt tacaggaaca cgaccgcttc cgtcagtgga acaacgaact
    39301 ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat cgcgagcatc tgcggcaatg
    39361 gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg cttgcggcga tcacgttgac
    39421 gttaaccgcc gatgaagttg ctaccgccct ggcgcaacat gctgagcaac gcccactgcg
    39481 tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa caaaaacgtc tggcgcagtt
    39541 aatggtcact atccagaatg tcactctgga acagacgcaa cgtaatgtcg cacttaacga
    39601 aatgcgccag cgttataaag aaaagacgca gcaacttgcc gatgtgaaaa ccatttgcga
    39661 gcaggaagcg cgcatcaaaa cgctggaagc ccagcgtgca cagttacagg cgggtcagcc
    39721 ttgtccactt tgtggttcca ccagccaccc ggcggtcgag gcgtatcagg cgctggagcc
    39781 tggtgttaat cagtctcgat tactggcgct ggaaaacgaa gttaaaaagc tcggcgaaga
    39841 aggtgcggcg ctgcgcgggc aactggatgc attaacgaag cagcttcagc gcgatgaaaa
    39901 cgaagcgcaa agcctccgac aagatgagca agcacttact caacaatggc aagccgtcac
    39961 ggccagcctc aatatcacct tgcagccaca ggacgatatt caaccgtggc tggatgcaca
    40021 agatgagcac gaacgccagc tgcggttact cagccaacga catgaattac aagggcagat
    40081 tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa attgaacaac gccagcagca
    40141 gctcttaacg gcattggcgg gttatgcact gacattgcca caggaagatg aagaagagag
    40201 ctggttggcg acacgtcagc aagaagcgca gagctggcag caacgccaga acgaattaac
    40261 cgcgctgcaa aaccgtattc agcagctgac gccgattctg gaaacgttgc cgcaaagtga
    40321 tgatctcccg cacagcgaag aaactgtggc gctggataac tggcggcagg ttcatgaaca
    40381 atgtctcgca ttacacagcc agcagcagac gttacagcaa caggatgttc tggcggcgca
    40441 aagtctgcaa aaagcccagg cgcagtttga caccgcgcta caggccagcg tctttgacga
    40501 tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca ctaacgcagc tggaacagct
    40561 caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg ctggtcactc agacagcaga
    40621 aacgctggca cagcatcaac aacaccgacc tgacgggttg gctctcactg tgacggtgga
    40681 gcagattcag caagagttag cgcaaactca ccaaaagttg cgtgaaaaca ccacgagtca
    40741 aggcgagatt cgccagcagc tgaagcagga tgcagataac cgtcagcaac aacaaacctt
    40801 aatgcagcaa attgctcaaa tgacgcagca ggttgaggac tggggatatc tgaattcgct
    40861 aataggttcc aaagagggcg ataaattccg caagtttgcc caggggctga cgctggataa
    40921 tttagtccat ctcgctaatc agcaacttac ccggctgcac gggcgctatc tgttacagcg
    40981 caaagccagc gaggcgctgg aagtcgaggt tgttgatacc tggcaggcag atgcggtacg
    41041 cgatacccgt accctttccg gcggcgaaag tttcctcgtt agtctggcgc tggcgctggc
    41101 gctttcggat ctggtcagcc ataaaacacg tattgactcg ctgttccttg atgaaggttt
    41161 tggcacgctg gatagcgaaa cgctggatac cgcccttgat gcgctggatg ccctgaacgc
    41221 cagtggcaaa accatcggtg tgattagcca cgtagaagcg atgaaagagc gtattccggt
    41281 gcagatcaaa gtgaaaaaga tcaacggcct gggctacagc aaactggaaa gtacgtttgc
    41341 agtgaaataa ctattcagca ggataatgaa tacagagggc gaattatctc ttggccttgc
    41401 tggtcgttat cctgcaagct atcactttat tggctacggt gattggtagc tgttctggtg
    41461 gttgtgatgg tggtatgaaa aaagtcattt tatctttggc tctgggcacg tttggtttgg
    41521 ggatggccga atttggcatt atgggcgtgc tcacggagct ggcgcataac gtaggaattt
    41581 cgattcctgc tgccgggcat atgatctcgt attatgcact gggggtggtg gtcggtgcgc
    41641 caatcatcgc actcttttcc agccgctact cactcaaaca tatcttgttg tttctggtgg
    41701 cgttgtgcgt cattggcaac gccatgttca cgctctcttc gtcttacctg atgctcgcca
    41761 ttggtcggct ggtatccggc tttccgcatg gcgcattttt tggcgtcgga gcgatcgtgt
    41821 tatcaaaaat tatcaaaccc ggaaaagtca ccgccgccgt ggcggggatg gtttccggga
    41881 tgacagtcgc caatttgctg ggcattccgc tgggaacgta tttaagtcag gaatttagct
    41941 ggcgttacac ctttttattg atcgctgttt ttaatattgc ggtgatggca tcggtctatt
    42001 tttgggtgcc agatattcgc gacgaggcga aaggaaagct gcgcgaacaa tttcactttt
    42061 tgcgcagccc ggccccgtgg ttaattttcg ccgccacgat gtttggcaac gcaggtgtgt
    42121 ttgcctggtt cagctacgta aagccataca tgatgtttat ttccggtttt tcggaaacgg
    42181 cgatgacctt tattatgatg ttagttgggc tagggatggt gttgggaaat atgctaagtg
    42241 gcaggatttc aggacgttat tcaccactgc gcattgcagc agtgactgac tttataattg
    42301 tactggcact gctgatgctc tttttctgcg gcggcatgaa aacaacgtcg cttatttttg
    42361 cttttatttg ttgcgcggga ttatttgccc tttcagcacc tctgcaaata ttattactac
    42421 aaaatgccaa aggcggagag ttattaggtg ccgcaggtgg gcaaatagcg tttaacctcg
    42481 gtagcgccgt cggcgcatat tgcggaggta tgatgctgac gccggggctg gcatataatt
    42541 acgtggcgct gcctgccgcc ctgctttcgt ttgctgcgat gtcatcgttg ctgctgtatg
    42601 gtcgctataa gcgccagcaa gcggcggata gtccggtgct ggcgaaacca ctggggtaga
    42661 ttactgtcac ggtgcgcgtt gcttattgcc ggatgcggcg tgaacgcctt atccgcccta
    42721 cgcggttctg gcacattttg taggcctgat aagacgcggc aagcgtcgca tcaggcatga
    42781 tgcgccaatt gcctacgttt tttactcttg tggccataac cacgcagcgc cgcgtacgcc
    42841 gctggaatca ccgtgcttcg ccttacgcac cggcgtttca cattcgccgc cgaagacaaa
    42901 ttgtttaatc aactgcccaa ccgtttgata taaacggtct acattgctca tcccgccccc
    42961 cagcacaatc acatccggat cgagaatatt cacgacatgt gccagcgatt ttgccagccg
    43021 cagctcgtag cgacgcaatg ccagttccgc taccggatcg ctttcttcaa ccaggcggat
    43081 aatttcactg cctttcagcg catgtccgct caaacgacga taatccgtcg cgaatcccgt
    43141 gcccgaaata aaggtttcaa tacaaccttg tttaccgcaa taacaaggga cttcctcgcg
    43201 ataacgcagt tcgtcttcgt ccatccacgg tagcggattg tgtccccact cacctgccgt
    43261 gccattgccg ccgatatgcg cccgcccatt gaatgccacg cccgcgccgc atcccgtgcc
    43321 gataatcacg gcaaataccg tctgcgctcc cgctgccgcg ccatctactg cttctgaaac
    43381 cgccagacag ttggcgtcat ttgccagccg cacttcccgc tgcaacctcg cgcttaagtc
    43441 tttatcgaat ggctgaccgt tgagccaggt tgaattggca ttcttcacca caccggtgta
    43501 aggcgaaatt gagccaggaa tgcccatacc taccgttccg cgctgccctg tcgcctgctc
    43561 cgccatatca accaacgtgg cgatcgtttc aatagtctgc cggtaatcat cacgcggcgt
    43621 gggcagacga tggcggtaca actgctcccc tgcatcgccc agtgcaatca cttcagtttt
    43681 ggtgccgcct aaatcgatac ctatacgcac ggtactctcc ttattttttt caatatcaat
    43741 agcgtagaga cggacaaccg gattggcaat gcaaggccgc cgacaattcg ttatcatgcc
    43801 cgctaaattt aacgacaagg ccgtggaaat tatcatgctg tggttcaaaa atttaatggt
    43861 ttaccgtctt agccgcgaga tttcgctgcg tgcagaagag atggaaaaac agctagcctc
    43921 gatggcattt accccatgcg gcagccagga catggcgaag atgggctggg ttcctccgat
    43981 gggatcgcac agcgatgcgt taacgcacgt tgccaatggt caaattgtta tctgcgcgcg
    44041 caaagaagaa aaaatcctcc cgtctccggt gattaaacag gcgctggaag cgaaaatcgc
    44101 caaactggaa gcggaacagg cgcgtaagct gaagaaaacc gaaaaagatt cgctgaaaga
    44161 cgaagtgctg cactctctgc tgccgcgtgc tttcagccgt tttagccaga caatgatgtg
    44221 gatcgacacg gttaacggtt tgattatggt ggactgcgcc agtgccaaaa aagcggaaga
    44281 tacgctggca ttactgcgta aaagcctggg gtcgttaccg gttgtaccat tgagcatgga
    44341 aaacccgatt gaactgacgc tgaccgaatg ggttcgctcc ggtagtgcgg cacagggctt
    44401 ccagctgctt gatgaagccg agctgaaatc gttgctggaa gatggcggcg tgatccgcgc
    44461 gaagaaacaa gatctgacca gcgaagagat caccaatcac attgaagccg gaaaagtggt
    44521 gacgaaactg gcgctcgact ggcagcagcg cattcagttt gtgatgtgcg acgatggttc
    44581 gctcaagcgt ctgaagttct gcgacgagct gcgcgatcaa aacgaagata tcgaccgtga
    44641 agatttcgcc cagcgttttg atgccgattt catcctgatg actggtgaac tggcagcgtt
    44701 aattcaaaac ctgattgaag gattaggtgg cgaagcacaa cgttaattgc tgattttcct
    44761 ttaatgccgg atgcgacgcc tgccgcgtct tatccggcgt acgaagccac accagggata
    44821 taattattcg ctacggcggg caataatttt tagcgcagca atattatgcg ttttatgctg
    44881 taacttgctc catggacgtt gtgtcattgt ttttcctcaa gccagtgacg cgcttcactg
    44941 cgaattttcc gcattgcccc ggttttcact aaatcccgta acagcgaaag atcgggttgt
    45001 tcgacaaata agagtaatga tggatcctct tttaaccacg tcgttttcac atcggcagcg
    45061 agctgtggat tattcatagc tagcgaccga tgccgagact cggtcaatgt cgttaacaac
    45121 gacgccgggg tatgtgcgtt gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc
    45181 agcaatgcac gccaccattc atcatctgat ttatctttta tcgtagccag ttccgcaaca
    45241 atatctggag atcttcactg atatttatat caaccttgat gaattatgaa tatatcggaa
    45301 attgaaaaaa tcgcagcaaa ggaaatatta ttatgggtta aagagaggat atttcagaaa
    45361 aatgccctcc cgttcaaggg gaaggcgagg aattacagat agcggcacag ataagaggtg
    45421 ggttcggcaa cttgcagatg aaactcactg tgaccgggaa cattaaacac cgaaccggct
    45481 tcatacactt gccagtcggt cgcgtcaggc agtaacacat tcagcgcgcc actgattacc
    45541 gtcatctctt ccggctcagc ggtgctgaag gtatattcgc cttcaaccat aacccccacg
    45601 ctggcgcgac cagtgctgct gctggaaaag ccgatcgatt tcactttgcc ggaaaagtac
    45661 tcattacttt gaagcataaa ctggccctgt ttggtagttg aacatctact tcactatagg
    45721 ggccagaggc gctggctgtc acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa
    45781 tcaatacgtt agacagcaag acaggaacat cgagctgttt ttgcagtaaa tcgcgatgat
    45841 gttggtgaaa tcctaaacaa tccagcataa tgacatcagc cccttttgcc agtaattctt
    45901 tcccggcatc aatgattttt tgttctgaat catgaatggg gttacccaat gaaaataccg
    45961 gcgatttctg caaaatttgc catttttgcg cctgaacggg cagcatctcc tcaaccggaa
    46021 cgataacccc cacctgatga tcttcaacaa tagaggaaac cagtggaggc aatattcgcg
    46081 acggctcaag aaagatcgta ttacgcgcag tcatactact aatgtttgct gtactcatta
    46141 ataaaatgac gtcataaccc tgattatcga gcacttcaac cacaccttgc aggtctcgct
    46201 ccactttacg acgcgaaaca tgggccagct ggttgtcatt taataatgtg agaatggtgt
    46261 cttcgcctgc ttctggcgcg tactccgcca tcacttcttc acgacttaac ttccccagca
    46321 ggctatgatg ggaaatatta tcttcgtcaa tgtattccgt caggagcggc aaaacctcct
    46381 gcataggtac aatgccgatg gtcaggatcg ccaacgacgc actcattgtt catccacctt
    46441 acttttcttt cgtcgttaat taccgggcaa gtgtgaagca ccactgctga cacttacttg
    46501 tgctacaaat gacaaaaagc gtagcagcag acgtgcacgg cataatgtaa agattccaaa
    46561 tgattccagt aatggattgt tattgtttaa tattctaatt attagaaaaa catgaattat
    46621 gaaaaatgtg acgcagatcc cttgtagtgg cactcagaat cccttccgag cagtctgatg
    46681 cggtcactct ttgtcagggt cctcatagcg ttttttcgca tccatcgctt cctgagccgt
    46741 cggatgttca ctgatcaacg agtctggttt aggatgatcg gctctgagtt ggtaccaggt
    46801 taccgtctgt cctggctttc ctttttcaat cgtcactata tatgcttcac gaggataagg
    46861 cggtttcgtt ggcatcgttc ttccttattt cacgggatga acgttaagta taggcgctct
    46921 aaaatcaaca attgatcgtc tgtgccaggg cgctacgaat ttcagaaatc acctggctgg
    46981 gttcgtttgt tgcgtcgatg ataatatgcg caacttcgcg atatagcgca tcgcgttctt
    47041 ccagcacttc ctgaacttct tcgctcagcg gttttcccgt taaagttggc cgtaaatctt
    47101 cttccggtgc agcttgcagt cggttaacca ggactgatac tggcgcacac aaataaacca
    47161 cgatcccgtt attttgcatg aagtgacgat taaattccgt cagaataatg ccgccgcctg
    47221 tagcgataac ggtggatggc gctgttaccg cttccagcgc cgccgtttct ctggcgcgaa
    47281 atcccgccca ctcttccctt tcgacgatct ccgcgaccgt catattgagc tgtgattgca
    47341 accactgatc ggtatcgaca aaccgacggt taagcgaatc ggcaagggcc attccgaccg
    47401 ttgttttacc acagccccga ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc
    47461 cccaataggt cgcaatgcgg cgaaagccgg tgtcatgaga atagcgatca taccatcaaa
    47521 ctagtacaat ttcgattgta aagaaaaaat tccacttaaa gtgaaaatct caatacatcc
    47581 cttactatac caataaatat tcaagaatga agtgtaaata ataaattaca tttagccacg
    47641 actacgttgc acttccagcc accacttctc cagctccgcg gcaaaggcct ggcggtcacg
    47701 ttgtgaaagg ctatctggtc cgccggtctg gatcccactg gcacgtaagg tatccataaa
    47761 atcgcgcatc gtcaggcgct cacgaatggt cgctggcgtg taacgttcgc cgcgcggatt
    47821 aagcgccgct gcgcctttct cgatggcttc agcagccaaa ggtatatctg cggtgatcac
    47881 caaatcgccc gcttcacact gccggacaat ttcgttatcg gcaacgtcga aacctgccgc
    47941 gacgcgcagc gtacgaataa atcgcgatgg cggcacgcgt aaactctggt ttgctaccag
    48001 taccagcggc atctgcatac gttccgccgc gcgatacaaa atctctttaa ttacattggg
    48061 acacgcgtcg gcatccaccc aaattgtcat aaagtcatcc tttgttgggt aatcctctat
    48121 tgtgtcgcgc ttttgccttc cggcatagtt ctgtttatgc ttctgccagc gattatcaaa
    48181 acaatgaatt tcacggcagg agtgaggcaa tggaaaagaa aatcggtttt attggctgcg
    48241 gcaatatggg aaaagccatt ctcagcggtc tgattgccag cggtcaggtg cttccagggc
    48301 aaatctgggt atacaccccc tccccggata aagtcgccgc cctgcatgac cagttcggca
    48361 tcaacgccgc agaatcggcg caagaagtgg cgcaaatcgc cgacatcatt tttgctgccg
    48421 ttaaaccagg catcatgatt aaagtgctta gcgaaatcac ctccagcctg aataaagact
    48481 ctctggtcgt ttctattgct gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc
    48541 atgaccggaa aattatccgc gccatgccga acactcccgc gctggttaat gccgggatga
    48601 cctccgtaac gccaaacgcg ctggtaaccc cagaagatac cgctgatgtg ctgaatattt
    48661 tccgctgctt tggcgaagcg gaagtaattg ctgagccgat gatccacccg gtggtcggtg
    48721 tgagcggttc ttcgccagcc tacgtattta tgtttatcga agcgatggcc gacgccgccg
    48781 tgctgggcgg gatgccacgc gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt
    48841 ccgcaaaaat ggtgctggaa acgggagaac atccgggggc actgaaagat atggtctgct
    48901 caccgggagg caccaccatt gaagcggtac gcgtactgga agagaaaggc ttccgtgccg
    48961 cagtgatcga agcgatgacg aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat
    49021 gactttcgcc ggacgtcagg ccgccacttc ggtgcggtta cgtccggctt tctttgcttt
    49081 gtaaagcgcc aaatctgccg atttcaacca ctcacgatag tgactcattt gtgggttcag
    49141 cggcgcaacc cccacactaa tccgtaaagt tacctgtggc gtattcggca agcgtaacgt
    49201 gtttagccct tcacgcaccc gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga
    49261 catgattact gcaaactcat cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt
    49321 aatttgtaac tgtcgggtaa gcgccacaat cgcttcatcg cccacatcat gaccccaggt
    49381 atcgttgatg ctcttgaaat ggtcgatatc gataatcagt aacgttgcat cgcgattatg
    49441 ccgccgacag ttatcaaatt cattgcgtaa catagtttcc caatgacgtc ggttatacac
    49501 gcccgtcatg ccgtcgcggg tactcatgac ctgcaacctg cgtttatgtt ccgccagttt
    49561 ggttgccgtc tggtagctga cccagccaaa cagcagagga taaacgacaa taatgggaag
    49621 ggagagccac cattccagcg gcgcactatt gaacgacacg gtaatgcccg taagctcgag
    49681 ggtgacaagg caggaaacca ccatcaacac cagacccgcg acaaacagac gggggccgcc
    49741 tgcccccatc aaattcagac acataatcat caacatagcg gtggaaggca gcacgtttac
    49801 gcccattacg cctacccaca ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc
    49861 ccggctaagc ggatcgacgg ccctgctcgc tatctgccag gctaaatgcg gccagacgaa
    49921 cgcccagccg accaacacca gccaccacca gcccggcggc gggtgcgaaa ccagcgttga
    49981 agcaatcggt aagaacatgc cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc
    50041 gaagcgcagc ccggaccgct gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg
    50101 cgccgccgct tttttgaaaa agttttcatc attcattatt tttgggaaca ttcagaaaca
    50161 attttcccaa attatagaga cggggctaaa ggcagaagta tgaaatttcg ggtgagccac
    50221 tttcgtagct cacccgttag cactatcagg tatcaggcgg ctttcttgag gcaggcactc
    50281 ataaacttat tacgatcatc acctttcaga gattgttgcg ttgcttgatt attacattcg
    50341 cgcatctttt gctgctgtgg cgtcaaactt ttttcaccag gcgcagactt gctgttcttc
    50401 aggcaatcac tcatgtaggt cttacgagca tcccctttca acgcctgcgc cgtcgcctgc
    50461 tgattacagg aggtcatacg ctgttgttgt ggggttaagg ttctctcggc agcgccgacg
    50521 gtggttaaaa aaaccagacc aaaaagcaag gtaaccagta atgttatttt catagcacca
    50581 tccctcttca tgttttaacc atgagcgttt gcgcccgtga tctgccatta agtctggttg
    50641 ctaacagcaa aaaaaccacc cggcagcgaa aattcactgc cgggcgcggt tttatttcag
    50701 ccccagagcg gctttcatgg tgtagaagag atcggtctgg tcggtcagtc caacaacatt
    50761 ggcggcatgc gggccatacg ccgcaatacg caactgactg ccggtatgtt cttgtgaatc
    50821 ctcttcggaa ttcccgtaac tcatcaccat cactgcgcca tctttagtat ttagcgcctg
    50881 agtgaggccc ggcgctttgg tatccggcgc aacaacctgg ctggcgtggg cgtgatcagc
    50941 ggtgactatg accagcgtgt taccctcctt tttagcgaat tccagcgccc gttgtacggc
    51001 ttcatcgaga tcgaccgtct cgccaatttg cccacaagga ttcgcagcgt gatcctgttt
    51061 atcgattgac gcaccttcaa cttgcaggaa aaatcctttc tcatttttac tcaacaattc
    51121 aatggctttg tcggtcatct gtgccagggt tggtacactg tcattacgtt gcggattagg
    51181 cgtacaggtg actgcgggct tatcgatatt gccgtggtac gttgctttcg gtcctagcca
    51241 gcgcactggc atattgccgt cagcaaacag gccaagcagg ggtttttgct gattcgcttc
    51301 cgtcaccgaa ttcagtgagg cagcatcgct caccaactga taaccacgcg cctgcgcctg
    51361 ttcacgcagc gtttttccct gccattcacc agcggttgcc gtttcagcaa aggtttttgc
    51421 gccgccgcca agcgtaacgt cggcacgagc gttaagcagc tgttcggtaa tcgatccttt
    51481 tccgcctttt tccagagcgt tacccggaca tttttcactg gtcgcgctcg gaccgtagca
    51541 tttgcgcgaa gtcacatgtg ccaccagcgc agcgggcgtg gcatcctgca actctgcggt
    51601 agaaacgtta ccggtcgcca gacctgcggc ttttgccatt tccagaatcg ttgggtgatc
    51661 tttttcgtga atatcgacgc ccagcgcgcc gttataggtt ttgacaccgg ttgaccaggc
    51721 ggttgctgat gcagccgagt cggtgacgta gtccggtttg ccggtttttt tattcagcgc
    51781 atagtgagtg tattgcccgg taagcggtaa ggcatcgata cctttaaaaa agccgcccgc
    51841 accttcggca taattacgtg cggcagtaat ttccgaatcc cccatcccat cgccaatcag
    51901 caaaataata ttttttgcag gtttatcgct aagagaatca cgcagagcgg cggtctgatc
    51961 acccgttaag cggcgagcac cgccgggtgc agtaatatcg ccctgagcag cccggttttc
    52021 cagaacaggc atttctggtg tccgggcttt tgtcacaggg gtaaacagta acggtaagag
    52081 tgccagtgca atagtgcttt gtttcacttt attttctcca tgtacaaata cattaaaaaa
    52141 taaaaacaaa gcgactataa gtctcggccg tgacaacttt atgacagctg ttgaaaagat
    52201 taacttttta ctgacgagga tgcttcaata acttctttac gtaatcgcgc agcagctccg
    52261 tatcgtagtc aggaatgctg gcatcgggct ttacctcgta cagcgccccc tctacctgat
    52321 caatcaaccg ctgttggtca ttttgcgcca tattgcgaag cattgcagtg acgataatct
    52381 ccaaagcttc tacctgcgca cacagttctt tcgactcttc ttctttttgg gcaagcttaa
    52441 ataacaactc agcaatgaga tttttcatgt ctgtatttcc ttatccaaag tatggagaag
    52501 ttaacattat gtttcacaac ttcatagtgt ctggaagtat tatttttatg acaaaggaaa
    52561 tactttgcgc aaatattttt tgtttgatta ccagcgaaac gtttctctta tttaaataat
    52621 tacccacgca aaacataggc ttttaattat caactt
//
LOCUS       DAZKHC020000022        51961 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZKHC020000022 DAZKHC020000000
VERSION     DAZKHC020000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 51961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.022"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1348)
                     /gene="uidB"
                     /locus_tag="ACHOSM_RS07770"
                     /old_locus_tag="pgaptmp_001554"
     CDS             complement(<1..1348)
                     /gene="uidB"
                     /locus_tag="ACHOSM_RS07770"
                     /old_locus_tag="pgaptmp_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HGU4558104.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQ"
     gene            complement(1345..3156)
                     /gene="uidA"
                     /locus_tag="ACHOSM_RS07775"
                     /old_locus_tag="pgaptmp_001555"
     CDS             complement(1345..3156)
                     /gene="uidA"
                     /locus_tag="ACHOSM_RS07775"
                     /old_locus_tag="pgaptmp_001555"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HGU4558105.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(3545..4135)
                     /gene="uidR"
                     /locus_tag="ACHOSM_RS07780"
                     /old_locus_tag="pgaptmp_001556"
     CDS             complement(3545..4135)
                     /gene="uidR"
                     /locus_tag="ACHOSM_RS07780"
                     /old_locus_tag="pgaptmp_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HGU4558106.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4356..5123)
                     /gene="hdhA"
                     /locus_tag="ACHOSM_RS07785"
                     /old_locus_tag="pgaptmp_001557"
     CDS             complement(4356..5123)
                     /gene="hdhA"
                     /locus_tag="ACHOSM_RS07785"
                     /old_locus_tag="pgaptmp_001557"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HGU4558107.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAELFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5235..6263)
                     /gene="malI"
                     /locus_tag="ACHOSM_RS07790"
                     /old_locus_tag="pgaptmp_001558"
     CDS             complement(5235..6263)
                     /gene="malI"
                     /locus_tag="ACHOSM_RS07790"
                     /old_locus_tag="pgaptmp_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HGU4558108.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6438..8030
                     /gene="malX"
                     /locus_tag="ACHOSM_RS07795"
                     /old_locus_tag="pgaptmp_001559"
     CDS             6438..8030
                     /gene="malX"
                     /locus_tag="ACHOSM_RS07795"
                     /old_locus_tag="pgaptmp_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HGU4558109.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIASIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            8040..9212
                     /gene="malY"
                     /locus_tag="ACHOSM_RS07800"
                     /old_locus_tag="pgaptmp_001560"
     CDS             8040..9212
                     /gene="malY"
                     /locus_tag="ACHOSM_RS07800"
                     /old_locus_tag="pgaptmp_001560"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HGU4558110.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSRSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9316..10317
                     /gene="add"
                     /locus_tag="ACHOSM_RS07805"
                     /old_locus_tag="pgaptmp_001561"
     CDS             9316..10317
                     /gene="add"
                     /locus_tag="ACHOSM_RS07805"
                     /old_locus_tag="pgaptmp_001561"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HGU4558111.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHLQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(10352..11392)
                     /gene="ydgJ"
                     /locus_tag="ACHOSM_RS07810"
                     /old_locus_tag="pgaptmp_001562"
     CDS             complement(10352..11392)
                     /gene="ydgJ"
                     /locus_tag="ACHOSM_RS07810"
                     /old_locus_tag="pgaptmp_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU4558112.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QTRELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLSQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11635..11760
                     /gene="blr"
                     /locus_tag="ACHOSM_RS07815"
                     /old_locus_tag="pgaptmp_001563"
     CDS             11635..11760
                     /gene="blr"
                     /locus_tag="ACHOSM_RS07815"
                     /old_locus_tag="pgaptmp_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HGU4558113.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            12033..12248
                     /gene="ydgT"
                     /locus_tag="ACHOSM_RS07820"
                     /old_locus_tag="pgaptmp_001564"
     CDS             12033..12248
                     /gene="ydgT"
                     /locus_tag="ACHOSM_RS07820"
                     /old_locus_tag="pgaptmp_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HGU4558114.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELLSGGRLFDLGQVPKSVWHYVQ"
     gene            12334..12774
                     /gene="ydgK"
                     /locus_tag="ACHOSM_RS07825"
                     /old_locus_tag="pgaptmp_001565"
     CDS             12334..12774
                     /gene="ydgK"
                     /locus_tag="ACHOSM_RS07825"
                     /old_locus_tag="pgaptmp_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HGU4558115.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            12851..13432
                     /gene="rsxA"
                     /locus_tag="ACHOSM_RS07830"
                     /old_locus_tag="pgaptmp_001566"
     CDS             12851..13432
                     /gene="rsxA"
                     /locus_tag="ACHOSM_RS07830"
                     /old_locus_tag="pgaptmp_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HGU4558116.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            13432..14010
                     /gene="rsxB"
                     /locus_tag="ACHOSM_RS07835"
                     /old_locus_tag="pgaptmp_001567"
     CDS             13432..14010
                     /gene="rsxB"
                     /locus_tag="ACHOSM_RS07835"
                     /old_locus_tag="pgaptmp_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HGU4558117.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            14003..16225
                     /gene="rsxC"
                     /locus_tag="ACHOSM_RS07840"
                     /old_locus_tag="pgaptmp_001568"
     CDS             14003..16225
                     /gene="rsxC"
                     /locus_tag="ACHOSM_RS07840"
                     /old_locus_tag="pgaptmp_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HGU4558118.1"
                     /translation="MLNLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVIEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAIE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAVPEEQVDP
                     RKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
     gene            16226..17284
                     /gene="rsxD"
                     /locus_tag="ACHOSM_RS07845"
                     /old_locus_tag="pgaptmp_001569"
     CDS             16226..17284
                     /gene="rsxD"
                     /locus_tag="ACHOSM_RS07845"
                     /old_locus_tag="pgaptmp_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HGU4558119.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTI
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAMQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            17288..17908
                     /gene="rsxG"
                     /locus_tag="ACHOSM_RS07850"
                     /old_locus_tag="pgaptmp_001570"
     CDS             17288..17908
                     /gene="rsxG"
                     /locus_tag="ACHOSM_RS07850"
                     /old_locus_tag="pgaptmp_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HGU4558120.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            17912..18607
                     /gene="rsxE"
                     /locus_tag="ACHOSM_RS07855"
                     /old_locus_tag="pgaptmp_001571"
     CDS             17912..18607
                     /gene="rsxE"
                     /locus_tag="ACHOSM_RS07855"
                     /old_locus_tag="pgaptmp_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HGU4558121.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            18607..19242
                     /gene="nth"
                     /locus_tag="ACHOSM_RS07860"
                     /old_locus_tag="pgaptmp_001572"
     CDS             18607..19242
                     /gene="nth"
                     /locus_tag="ACHOSM_RS07860"
                     /old_locus_tag="pgaptmp_001572"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGU4558122.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVNCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            19853..21355
                     /gene="dtpA"
                     /locus_tag="ACHOSM_RS07865"
                     /old_locus_tag="pgaptmp_001573"
     CDS             19853..21355
                     /gene="dtpA"
                     /locus_tag="ACHOSM_RS07865"
                     /old_locus_tag="pgaptmp_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HGU4558123.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            21460..22065
                     /gene="gstA"
                     /locus_tag="ACHOSM_RS07870"
                     /old_locus_tag="pgaptmp_001574"
     CDS             21460..22065
                     /gene="gstA"
                     /locus_tag="ACHOSM_RS07870"
                     /old_locus_tag="pgaptmp_001574"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HGU4558124.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(22109..22972)
                     /gene="pdxY"
                     /locus_tag="ACHOSM_RS07875"
                     /old_locus_tag="pgaptmp_001575"
     CDS             complement(22109..22972)
                     /gene="pdxY"
                     /locus_tag="ACHOSM_RS07875"
                     /old_locus_tag="pgaptmp_001575"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HGU4558125.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(23031..24305)
                     /gene="tyrS"
                     /locus_tag="ACHOSM_RS07880"
                     /old_locus_tag="pgaptmp_001576"
     CDS             complement(23031..24305)
                     /gene="tyrS"
                     /locus_tag="ACHOSM_RS07880"
                     /old_locus_tag="pgaptmp_001576"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGU4558126.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDKGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSAKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(24434..25090)
                     /gene="pdxH"
                     /locus_tag="ACHOSM_RS07885"
                     /old_locus_tag="pgaptmp_001577"
     CDS             complement(24434..25090)
                     /gene="pdxH"
                     /locus_tag="ACHOSM_RS07885"
                     /old_locus_tag="pgaptmp_001577"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HGU4558127.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(25149..25478)
                     /gene="mliC"
                     /locus_tag="ACHOSM_RS07890"
                     /old_locus_tag="pgaptmp_001578"
     CDS             complement(25149..25478)
                     /gene="mliC"
                     /locus_tag="ACHOSM_RS07890"
                     /old_locus_tag="pgaptmp_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HGU4558128.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(25576..26685)
                     /gene="anmK"
                     /locus_tag="ACHOSM_RS07895"
                     /old_locus_tag="pgaptmp_001579"
     CDS             complement(25576..26685)
                     /gene="anmK"
                     /locus_tag="ACHOSM_RS07895"
                     /old_locus_tag="pgaptmp_001579"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HGU4558129.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQVGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTVV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            26959..27426
                     /gene="slyB"
                     /locus_tag="ACHOSM_RS07900"
                     /old_locus_tag="pgaptmp_001580"
     CDS             26959..27426
                     /gene="slyB"
                     /locus_tag="ACHOSM_RS07900"
                     /old_locus_tag="pgaptmp_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HGU4558130.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(27473..27907)
                     /gene="slyA"
                     /locus_tag="ACHOSM_RS07905"
                     /old_locus_tag="pgaptmp_001581"
     CDS             complement(27473..27907)
                     /gene="slyA"
                     /locus_tag="ACHOSM_RS07905"
                     /old_locus_tag="pgaptmp_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HGU4558131.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            28108..28344
                     /gene="ydhI"
                     /locus_tag="ACHOSM_RS07910"
                     /old_locus_tag="pgaptmp_001582"
     CDS             28108..28344
                     /gene="ydhI"
                     /locus_tag="ACHOSM_RS07910"
                     /old_locus_tag="pgaptmp_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HGU4558132.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            28347..29204
                     /gene="ydhJ"
                     /locus_tag="ACHOSM_RS07915"
                     /old_locus_tag="pgaptmp_001583"
     CDS             28347..29204
                     /gene="ydhJ"
                     /locus_tag="ACHOSM_RS07915"
                     /old_locus_tag="pgaptmp_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGU4558133.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            29204..31216
                     /gene="ydhK"
                     /locus_tag="ACHOSM_RS07920"
                     /old_locus_tag="pgaptmp_001584"
     CDS             29204..31216
                     /gene="ydhK"
                     /locus_tag="ACHOSM_RS07920"
                     /old_locus_tag="pgaptmp_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU4558134.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGCIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGLFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(31217..31738)
                     /gene="sodC"
                     /locus_tag="ACHOSM_RS07925"
                     /old_locus_tag="pgaptmp_001585"
     CDS             complement(31217..31738)
                     /gene="sodC"
                     /locus_tag="ACHOSM_RS07925"
                     /old_locus_tag="pgaptmp_001585"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGU4558135.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(31819..32715)
                     /gene="ydhF"
                     /locus_tag="ACHOSM_RS07930"
                     /old_locus_tag="pgaptmp_001586"
     CDS             complement(31819..32715)
                     /gene="ydhF"
                     /locus_tag="ACHOSM_RS07930"
                     /old_locus_tag="pgaptmp_001586"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HGU4558136.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(32764..33003)
                     /gene="ydhL"
                     /locus_tag="ACHOSM_RS07935"
                     /old_locus_tag="pgaptmp_001587"
     CDS             complement(32764..33003)
                     /gene="ydhL"
                     /locus_tag="ACHOSM_RS07935"
                     /old_locus_tag="pgaptmp_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HGU4558137.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            33106..33705
                     /gene="nemR"
                     /locus_tag="ACHOSM_RS07940"
                     /old_locus_tag="pgaptmp_001588"
     CDS             33106..33705
                     /gene="nemR"
                     /locus_tag="ACHOSM_RS07940"
                     /old_locus_tag="pgaptmp_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HGU4558138.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            33742..34839
                     /gene="nemA"
                     /locus_tag="ACHOSM_RS07945"
                     /old_locus_tag="pgaptmp_001589"
     CDS             33742..34839
                     /gene="nemA"
                     /locus_tag="ACHOSM_RS07945"
                     /old_locus_tag="pgaptmp_001589"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HGU4558139.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIKEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            34920..35327
                     /gene="gloA"
                     /locus_tag="ACHOSM_RS07950"
                     /old_locus_tag="pgaptmp_001590"
     CDS             34920..35327
                     /gene="gloA"
                     /locus_tag="ACHOSM_RS07950"
                     /old_locus_tag="pgaptmp_001590"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HGU4558140.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            35430..36077
                     /gene="rnt"
                     /locus_tag="ACHOSM_RS07955"
                     /old_locus_tag="pgaptmp_001591"
     CDS             35430..36077
                     /gene="rnt"
                     /locus_tag="ACHOSM_RS07955"
                     /old_locus_tag="pgaptmp_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HGU4558141.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            36170..40786
                     /gene="lhr"
                     /locus_tag="ACHOSM_RS07960"
                     /old_locus_tag="pgaptmp_001592"
     CDS             36170..40786
                     /gene="lhr"
                     /locus_tag="ACHOSM_RS07960"
                     /old_locus_tag="pgaptmp_001592"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HGU4558142.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAEFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVIATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNTETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLCELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(40837..41184)
                     /gene="grxD"
                     /locus_tag="ACHOSM_RS07965"
                     /old_locus_tag="pgaptmp_001593"
     CDS             complement(40837..41184)
                     /gene="grxD"
                     /locus_tag="ACHOSM_RS07965"
                     /old_locus_tag="pgaptmp_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HGU4558143.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            41518..42333
                     /gene="mepH"
                     /locus_tag="ACHOSM_RS07970"
                     /old_locus_tag="pgaptmp_001594"
     CDS             41518..42333
                     /gene="mepH"
                     /locus_tag="ACHOSM_RS07970"
                     /old_locus_tag="pgaptmp_001594"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HGU4558144.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTQKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSEQKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            42461..43042
                     /gene="sodB"
                     /locus_tag="ACHOSM_RS07975"
                     /old_locus_tag="pgaptmp_001595"
     CDS             42461..43042
                     /gene="sodB"
                     /locus_tag="ACHOSM_RS07975"
                     /old_locus_tag="pgaptmp_001595"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HGU4558145.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(43188..44363)
                     /gene="ydhP"
                     /locus_tag="ACHOSM_RS07980"
                     /old_locus_tag="pgaptmp_001596"
     CDS             complement(43188..44363)
                     /gene="ydhP"
                     /locus_tag="ACHOSM_RS07980"
                     /old_locus_tag="pgaptmp_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4558146.1"
                     /translation="MKINYPLLALALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAA
                     GMLISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSR
                     ISTSLNHGAFLGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGW
                     RMSFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGA
                     MFTLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLL
                     LLMVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIG
                     AFNLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(44529..44618)
                     /gene="cydH"
                     /locus_tag="ACHOSM_RS07985"
                     /old_locus_tag="pgaptmp_001597"
                     /gene_synonym="ynhF"
     CDS             complement(44529..44618)
                     /gene="cydH"
                     /locus_tag="ACHOSM_RS07985"
                     /old_locus_tag="pgaptmp_001597"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HGU4558147.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            44917..45942
                     /gene="purR"
                     /locus_tag="ACHOSM_RS07990"
                     /old_locus_tag="pgaptmp_001598"
     CDS             44917..45942
                     /gene="purR"
                     /locus_tag="ACHOSM_RS07990"
                     /old_locus_tag="pgaptmp_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HGU4558148.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(45939..46871)
                     /gene="punR"
                     /locus_tag="ACHOSM_RS07995"
                     /old_locus_tag="pgaptmp_001599"
     CDS             complement(45939..46871)
                     /gene="punR"
                     /locus_tag="ACHOSM_RS07995"
                     /old_locus_tag="pgaptmp_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HGU4558149.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            46984..48195
                     /gene="punC"
                     /locus_tag="ACHOSM_RS08000"
                     /old_locus_tag="pgaptmp_001600"
     CDS             46984..48195
                     /gene="punC"
                     /locus_tag="ACHOSM_RS08000"
                     /old_locus_tag="pgaptmp_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HGU4558150.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYFPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            48487..49635
                     /gene="cfa"
                     /locus_tag="ACHOSM_RS08005"
                     /old_locus_tag="pgaptmp_001601"
     CDS             48487..49635
                     /gene="cfa"
                     /locus_tag="ACHOSM_RS08005"
                     /old_locus_tag="pgaptmp_001601"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HGU4558151.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(49675..50316)
                     /gene="ribE"
                     /locus_tag="ACHOSM_RS08010"
                     /old_locus_tag="pgaptmp_001602"
     CDS             complement(49675..50316)
                     /gene="ribE"
                     /locus_tag="ACHOSM_RS08010"
                     /old_locus_tag="pgaptmp_001602"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGU4558152.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            50531..51904
                     /gene="mdtK"
                     /locus_tag="ACHOSM_RS08015"
                     /old_locus_tag="pgaptmp_001603"
     CDS             50531..51904
                     /gene="mdtK"
                     /locus_tag="ACHOSM_RS08015"
                     /old_locus_tag="pgaptmp_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HGU4558153.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
BASE COUNT        12299 a        13506 c        13131 g        13025 t
ORIGIN      
        1 gctgctgcac ttttttacga ttatcaattt caaccacgat ttctttgaat tttttatccg
       61 tgagcggata aaaccagata ataacgaatg ccagtagcat aaatccgcaa ggtactaagg
      121 caattgatgt gcggatgccc ataataactt ccggcgtttg cacctgattg gcgatatatc
      181 cgcttaatcc caaaataaag gcaggaattg aacctccgat tgcctgaccg catttacggg
      241 taaatgagaa tagtgaatag gtgagccctt caattcgcac gccggtcagg tattcaccgt
      301 attctacggt atcagcttcc agtgcccaca tcacagtcat agtaacgccc tgaccaattg
      361 aagcgatggc caacgcaacc aacgccaccg gcagtgacca gacggaaacc cagaagaata
      421 gcagataacc gcaggttccc agcaaagcgc caatcaggaa ggtattcttt ttaccgatcc
      481 tcgcgaccat ccccggcacc agcggtgccg atgccacagt accaaccagg ttttgcacca
      541 gtaccagcac agtgaacagc ccggtatcat ttaacacata gcgcacgtag aacaacgacg
      601 aggcgctgac cgcgaaggtc gaaatcagca cacacagcgc accgatgcac aacataaaca
      661 gcgggcgatt ccgtttcagg gtttgcagac tgatcttcaa tgacggctgc gccacgatac
      721 gtaccacatt ctcacgcgtc gatttgaagc agatgaagta aagcaccatt ccggcaatcg
      781 ccagcacaat cgtccagaaa tggtataccg acaccatctc ttccgggctg gagttcttaa
      841 tgctcggtcc tatcagaaat gccaggcaga caaaggttaa tgaagcggca atcccgcgag
      901 ccgcgcccag acgggcgcgg gattgtggtt gttgggtcat cgcggtagca agtgaaccat
      961 aaggaatatt caccaggctg tagcaaagcc cgaggcccat gtaggtcaaa tatgcataca
     1021 ccactttgct accatggctc cagtcggtcg gcacccagaa taccagcacg ctgaagatca
     1081 ttaacggcgc agtaccgaag agtaaaaacg ggcggaattt tccccagcgg gtattcacac
     1141 tgtccaccac tcgtccggca aagacgtcgg cgaaggcatc gaatacccgc accagtaaca
     1201 gcatggtgcc cgccgcagcg gcaccgacgc cagcgacgtc ggtgtagtaa ctcaacagga
     1261 agagcgcccc cattgcgaag gcgaagttat tggcgacgtc accgaggctg tagccgacga
     1321 tggtgcgcca ggagagttgt tgattcattg tttgcctccc tgctgcggtt tttcaccgaa
     1381 gttcatgcca gtccagcgtt tttgcagcag aaaagccgcc gacttcggtt tgcggtcgcg
     1441 ggtgaagatc cccttcttgt taccgccaac gcgcaatatg ccttgcgagg tcgcaaaatc
     1501 ggcgaaattc catacctgtt caccgacgac ggcgctgacg cgatcaaaga cgcggtgata
     1561 catatccagc catgcacact gatactcttc actccacatg tcggtgtaca ttgagtgcag
     1621 cccggctaac gtatccacgc cgtattcggt gatgataatc ggctgatgca gtttctcctg
     1681 ccaggccaga agttcttttt ccagtacctt ctctgccgtt tccaaatcgc cgctttggac
     1741 ataccatccg taataacggt tcaggcacag cacatcaaag agatcgctga tggtatcggt
     1801 gtgagcgtcg cagaacatta cattgacgca ggtgatcgga cgcgtcgggt cgagtttacg
     1861 cgttgcttcc gccagtggcg cgaaatattc ccgtgcacct tgcggacggg tatccggttc
     1921 gttggcaata ctccacatca ccacgcttgg gtggtttttg tcacgcgcta tcagctcttt
     1981 aatcgcctgt aagtgcgctt gctgagtttc cccgttgact gcctcttcgc tgtacagttc
     2041 tttcggcttg ttgcccgctt cgaaaccaat gcctaaagag aggttaaagc cgacagcagc
     2101 agtttcatca atcaccacga tgccatgttc atctgcccag tcgagcatct cttcagcgta
     2161 agggtaatgc gaggtacggt aggagttggc cccaatccag tccattaatg cgtggtcgtg
     2221 caccatcagc acgttatcga atcctttgcc tcgcaaatcc gcatcttcat gacgaccaaa
     2281 gccagtaaag tagaacggtt tgtggttaat caggaactgt tcgcccttca ctgccactga
     2341 gcggatgccg acgcgaagcg ggtagatatc acactctgtc tggcttttgg ctgtgacgca
     2401 cagttcatag agataacctt cacccggttg ccagaggtgc ggattcacca cttgcaaagt
     2461 cccgctagtg ccttgtccag ttgcaaccac ctgttgatcc gcatcacgca gttcaacgct
     2521 gacatcacca ttggccacca cctgccagtc aacagacgcg tggttacagt cttgcgcgac
     2581 atgcgtcacc acggtgatat cgtccaccca ggtgttcggc gtggtgtaga gcattacgct
     2641 gcgatggatc ccggcatagt taaagaaatc atggaagtaa gactgctttt tcttgccgtt
     2701 ttcgtcggta atcaccattc ccggcgggat agtctgccag ttcagttcgt tgttcacaca
     2761 aacggtgata cgtacacttt tcccggcaat aacatacggc gtgacatcgg cttcaaatgg
     2821 cgtatagccg ccctgatgct ccatcacttc ctgattattg acccacactt tgccgtaatg
     2881 agtgaccgca tcgaaacgca gcacgatacg ctggcctgcc caacctttcg gtataaagac
     2941 ttcgcgctga taccagacgt tgcccgcata attacgaata tctgcatcgg cgaactgatc
     3001 gttaaaactg cctggcacag caattgcccg gctttcttgt aacgcgcttt cccaccaacg
     3061 ctgatcaatt ccacagtttt cgcgatccag actgaatgcc cacaggccgt cgagtttttt
     3121 gatttcacgg gttggggttt ctacaggacg taacataagg gactcctcat taagttaaaa
     3181 atactggtca acctttaatc tgattggtta accaattctg tgttgcacac agttatctgt
     3241 gagaaaccca tggaagagaa atggaaataa aaagaactga caccgcgatc aaaaaaccaa
     3301 aaactgcaat acccacagcg tgatattgat cgcattaatc tttaaaacgg cgttgacaga
     3361 agccacaaaa taagcaatca aacgttcgct cgagcatatt gggaacgata aactctcatg
     3421 acatatggct acggtgaata tttttggggg ggaattctcc gatcccggct agattgtatt
     3481 ttgtacaggg aaattaaagt atgaaacgcg ggcacagtta tcaccagccc gcgaaatgag
     3541 aggatcagga tgcggttaag ataccgccaa tcatcgcgcg taatccctga gcaaacgacg
     3601 cttcacgcgc cagatcttcc gcttcgatat cggccagtac accgtaggtc agatccagca
     3661 gtaaacgtga agtcattgac gtgttgatgt ctgggcttat ctcgcctttt tgctgggcat
     3721 cacgcatccg ctgggcaaca aattcagtga tcgtcatatg cttattttta agcatggcgg
     3781 cgacctgtgg gttacgcatc ccttccgcca taatttcgac caccagcgcc cgttgcccaa
     3841 aggcttcatg ggtgagagag acaatagact cgaccatata gtcaacaaaa tgaatccctt
     3901 caatcggttc ccggaaacgg gccagcgccc tctcctggtc ctgtaagata atcgcctgaa
     3961 tcaaggcttc tttggagatg aaatggtgat agagcgtccc gggactaatg gcgcaggatt
     4021 tacagatggc tttcatcgag gcactgtgaa atccattttc tgaaaaaatc tctctggcag
     4081 cattgaggat ccgggtccgt gtcggttgtg cttcagtctg catgttatcc atcataaaac
     4141 cattatagtt agcgtgaata actagtttac cgcgttgtca ggtgagcgcc tatcattgct
     4201 gttgaatagt gcgaaggcac actctattcg tatacttttc actctgaaat tggttaacca
     4261 atagccgtga tttggctgcc agttatttag cagagacgtt gatgagttta tcccggtaaa
     4321 caacacgcac ggttgtttac cggtccgtta gtgtattaat tgagctcctg taccccacca
     4381 ccggagacgg tgagaatttg tccgcttacc cagctggctg caggcgagca aaggaacagc
     4441 tctgcgttag caatatcttg cggttggccc agacgtctga tcggcgtgtg ctgtaacatt
     4501 ttttgttcaa tttctggtgt aataacggat ttcagggcat cggttaatat tgcccccggc
     4561 gcaatgccat ttacccgaat atttttttca cccaggtcaa acgccatatt tctgaccaga
     4621 tgactggctg cagctttaga tgatgcatag gaagtcatgt ttatattttt attttctgcc
     4681 gccatagaag tgatggtcag aataacgcca ccgccatttt tttccatttc tggcgcaaca
     4741 agttgtgaca gatggaaaaa agaaaacaca ttcagttcat aagcacggcg aaaatccgcc
     4801 attggcatat caaacggttt aggtccaccg ccaccggcgt tgttaaccag aatatcaact
     4861 ttacccagct tactgatagc aaagtctgcc agtgcagaga gttcctgttc ggaagtaata
     4921 tcacaacggc aggcaaatgc ctgaccaccc agttgttgaa tttcgtctac aacatggtta
     4981 gctgcgtcgg cgttaatatc actgaccacc acagatgcgc cagctgtcgc gaatgtaatg
     5041 gcgatttctt taccaatacc tgcacccgca cctgtgatga tggcgcattt tccgtctagt
     5101 ctcaggttgt cagaattaaa cacataaacc tcctgtgaac ttcagtaaaa ataaaaggca
     5161 ccgcctgtgt gccacatttt ttatcattaa ccttgaggaa aggacactac caggtatagc
     5221 agataaagag aggattattt cgctgcaatg agccgggcgg gaataataag attgcgtgaa
     5281 tgcgtctctt catgggtgat tttttgcatc atgcgatccg caagtgtgat accaagttcc
     5341 cgcgctggcg tgctggccca ggtaacaggt atatcatcaa gtgtggttgg tgtcgcatcg
     5401 gtaaatgccg ccagcgaaac ctgttgctca aagtaacgat cgactccgct ttccccgctt
     5461 tgccgccctg ctttgagcaa accaaaccat gcccccatcg caatagtttc gttatagcaa
     5521 accacggcac tgatggtcgg gttatgacgt aaaagcgccg tgatagcttc cgcagcttgc
     5581 ttctggctgg aagtgcactc caacacccaa tcgctatgaa acggcaggcc aaattttagt
     5641 agagttgcac aatagccccc cacccgttct gcacgggtta atgaggaact ttgccctccc
     5701 agccaggcga tccgctgatg cccattgcga atgagatgct ccgtcaacaa ctgtgcagcc
     5761 tgcatgttgt ccgggcgaac cgtatcaaca tcatcaagat aactggcacg ggaagcgaaa
     5821 atcaccggga tagctttttc ttctgccatc cgtcgcaggt catcgctact tcctgcagct
     5881 ccggcaatta ccacaccatc gacaccctga ttcagtaaca gtgaaaaccg ctgtgccagc
     5941 tgctcaccgt ctttaccgcc gtgaagcaaa aaaaccatcc gtccctgcgc ttccagagct
     6001 tccgtcaatc cggccgtcaa ttcggcgtaa aacggcgcag ataaatcacg gacgatcaaa
     6061 ccaatgacgc cgctttgccc gccgcgcagc gccgacgcct ggcgattgcg cacaaatccc
     6121 agctcttcaa tggcggcgtt aacgcgttct cctgtggcgg tagagattcg ccctttgcca
     6181 ctaagcacca gcgaaacggt acttaccgac acgcccgcag ccagcgcaac atcatgaatg
     6241 gttatttttt tggcggtagc catgcagatg acctactccc tgattatgtg acagataaaa
     6301 cgttttacct tttatttatc ttatacccgc tattatcgtt gcgtaatgtg atttatgcct
     6361 cactaaaatt tgataaaacg ttttatcttc tcgcgcaatt tactgaatcc agattgttct
     6421 ctacgaggag tcgttttatg acggcgaaaa cagcaccgaa agtcacgctg tgggagttct
     6481 tccagcagtt aggcaaaacc ttcatgttac ccgtggcatt attgtcgttc tgcggcatta
     6541 tgctcggcat tggtagttct cttagcagcc atgatgtcat aaccctgatc ccggtcctgg
     6601 gcaaccccgt gttgcaggct atctttacct ggatgagtaa gattggctcg tttgctttta
     6661 gtttcctgcc tgtcatgttc tgtatcgcca tcccgctggg cctggcacgc gaaaataaag
     6721 gcgtagcggc attcgctggc ttcgtcggtt atgcggtaat gaacctcgcg gtaaacttct
     6781 ggttgaccaa taaaggcatt ctgccaacca cggatgccgc ggttctgaaa gccaataaca
     6841 tccagagcat tcttgggatc cagtcgatcg ataccgggat cctcggtgcg gtgatcgcca
     6901 gtattatcgt ctggatgctg catgagcgtt tccataatat ccgcctgccg gatgcgctgg
     6961 cattcttcgg cggtacgcgc ttcgtaccaa ttatctcctc gctggtgatg ggccttgtcg
     7021 gcctggtgat tccattagtc tggccgattt tcgccatggg tattagcggc ttaggccaca
     7081 tgatcaatag cgcgggtgat ttcggaccga tgctgtttgg taccggtgaa cgtctgctgt
     7141 tgccgtttgg tctgcatcac attctggtgg cattaattcg ctttaccgac gcaggcggca
     7201 cgcaggaagt ctgcggtcaa accgtcagcg gcgcactgac catcttccag gcgcaattga
     7261 gttgcccgac cactcacggt ttttctgaaa gcgccacgcg tttcctttcg caaggcaaaa
     7321 tgcctgcgtt tctcggcggt ctgccaggtg cagcgttagc gatgtatcac tgcgcgcgcc
     7381 cggaaaatcg ccataaaatt aaagggctgc tgatttctgg cctgatcgcc tgtgtcgttg
     7441 gcggcacgac cgaaccgctg gaattcctgt tcctgttcgt ggcgccagtt ctgtatgtca
     7501 tccacgcgct gttaaccggc ctcggcttca ccgtcatgtc tgtgctcggc gtcaccatcg
     7561 gtaataccga cggcaatatc atcgacttcg tggtgttcgg tattttgcat ggtctgtcaa
     7621 ccaagtggta catggtgcca gtggtggcgg caatctggtt tgtcgtttac tacgtcatct
     7681 tccgtttcgc tatcacccgc ttcaatctga aaaccccggg gcgcgatagc gaagtggcca
     7741 gttcaatcga aaaagccgtt gccggtgcgc cgggtaaatc aggttacaac gttccggcaa
     7801 tcctcgaagc attaggcggt gccgacaata ttgtcagcct cgataactgc attacccgtc
     7861 tgcgtttgtc tgtgaaagat atgtcgcttg ttaatgtgca ggcactgaag gacaatcggg
     7921 caattggcgt agtacaactt aatcaacata acctgcaggt tgttatcggg ccacaagttc
     7981 agtcagtaaa agatgaaatg gccggtctga tgcatactgt ccaggcataa ggataagata
     8041 tgttcgattt ttcaaaggtc gtggatcgtc atggcacatg gtgcacgcag tgggattatg
     8101 tcgctgaccg tttcggcact gctgacctgt taccgttcac gatttcagac atggattttg
     8161 ccactgcccc ctgcattatc gaggcgctga atcagcgcct gatgcacggc gtatttggct
     8221 acagccgctg gaaaaacgat gagtttctcg cggctattgc ccactggttt tccacccagc
     8281 attacaccgc aattgatacg cagacggtgg tgtatggccc ttctgtcatc tatatggttt
     8341 cagaactgat tcgtcagtgg tctgaaacag gtgaaggcgt ggtgatccac acgcccgcct
     8401 atgacgcatt ttacaaggcc attgaaggta accagcgcac agtaatgccc gttgctttag
     8461 agaagcaggc tgacggctgg ttttgcgata tgggcaagct ggaagccgtg ttggcgaaac
     8521 cagaatgtaa aattatgctc ctgtgtagcc cacagaatcc taccgggaaa gtgtggacgt
     8581 gcgatgagct ggagatcatg gctgacctat gcgagcgtca tggtgtgcgg gttatttccg
     8641 atgaaatcca tatggatatg gtctggggcg agcagccgca tattccctgg agtaatgtgg
     8701 cgcggggaga ctgggcgttg ctgacgtcgg gctcgaaaag tttcaatatt cccgcgctga
     8761 ccggtgctta cgggattata gaaaatagca gaagccgtga tgcctattta tcggcactga
     8821 aaggacgtga tgggctttct tccccttcgg tactggcgtt aactgcccat atcgccgcct
     8881 atcagcaagg tgcaccgtgg ctggatgcct tacgcgtcta tctgaaagat aacctgacgt
     8941 atatctcaga taaaatgaac gccgcgtttc ctgaactcaa ctggcagatc ccgcaatcca
     9001 cttatctggc ctggcttgat ctacgtccgt tgaatattga cgacaacgcg ttgcaaaaag
     9061 cgcttatcga gcaagaaaaa gtcgcgatca tgccggggta tacctatggt gaagaaggtc
     9121 gtggttttgt ccgtctcaat gccggctgcc cacgttcgaa actggaaaaa ggtgtggctg
     9181 gattaataaa cgccatccgc gctgttcgct aaccccaatt gcgcaacgta aaaaatcgtt
     9241 gcgcaatcgt ggatttttac cctgctttgt ttttataatg gtgcgcactt ttatattcag
     9301 aaaaagagtg cgaccatgat tgataccacc ctgccattaa ctgatatcca tcgccacctt
     9361 gatggcaaca ttcgtcccca gaccattctt gaacttggcc gccagtataa tatctcgctt
     9421 cctgcacaat ccctggaaac actgattccc caccttcagg tcattgccaa cgaacccgat
     9481 ctggtgagct ttctgaccaa acttgactgg ggcgttaaag ttctcgcctc tcttgatgcc
     9541 tgtcgccgcg tggcatttga aaacattgaa gatgcagccc gtcacggcct gcactatgtc
     9601 gagctgcgtt tttcaccagg ctacatggca atggcacatc agctgcctgt agcgggtgtt
     9661 gtcgaagcgg tgatcgatgg cgtacgtgaa ggttgccgca cctttggtgt gcaggcgaag
     9721 cttatcggca ttatgagccg gaccttcggc gaagccgcct gtcagcaaga gctggaggcc
     9781 tttttagccc accgtgacca gattaccgca cttgatttag ccggtgatga acttggtttc
     9841 ccgggaagtc tgttcctttc tcacttcaac cgcgcgcgtg atgcgggctg gcatattacc
     9901 gtccatgcag gcgaagctgc cgggccggaa agcatctggc aggcgattcg tgaactgggt
     9961 gcggagcgta ttggacatgg cgtaaaagcc attgaagatc gggcgctgat ggattttctc
    10021 gccgagcaac aaattggtat tgaatcctgt ctgacctcca atattcagac cagcaccgta
    10081 gcagagctgg ctgcacatcc gctgaaaacg ttccttgagc atggcattcg tgccagcatt
    10141 aacactgacg atcccggcgt acagggagtg gatatcattc acgaatatac cgttgccgcg
    10201 ccagctgctg ggttatcccg cgagcaaatc cgccaggcac agattaatgg tctggaaatg
    10261 gctttcctca gcgctgagga aaaacgcgca ctgcgagaaa aagtcgccgc gaagtaacaa
    10321 aatggatggt gcaaattgca ccatccattt ttcatgcaag gcacaaagtc gcgcgatgtt
    10381 tggcggattc gatgcccagc tcaatcaact ccattacctg gattgcttgg cttgccggaa
    10441 ccggattttc accatcgcca tttaacgcat cacgaatagc cgcatagtaa gccggataat
    10501 tcccaggcac cgtcaacagc gtttcttcga cacgttcctc accttccacg cgggtaagta
    10561 cgccatcacg catatcgtag ccccagtctt cctgcgatag acgctcgcca tttttcagac
    10621 gttcttcctg tggatcgagg ccatatttca cataactgcc tcgtgatcca tgcacgatat
    10681 accgtgctga ctcagcggct gccagcatgg taccgtgtaa aatgactcgc cgctgtggat
    10741 aggacaagat ggcgtggaaa taatcggtcg actgcgctcc gggccgtaac tgtgccaaat
    10801 ctaccgtcat gctgaccggt aaaccaaaaa gcgtaatggc ctgatcaaga agatgtggtg
    10861 ctaaatcgta ccagataccg ctgcctgggc cgccctgttc acgccaacga tcgcgcacct
    10921 gcggacggaa gcggtcaaaa tgagactcaa agtaagcaac ttcacccagc acgccttccg
    10981 cgagtaaacc ttttagcgtc aagaaatcgc tatcccagcg acggttatgg aatacagaca
    11041 gcacacgccc caggcttttt gccagcgcat ccagctctcg cgtttgtgac agtgtcacgg
    11101 taaagggttt atcaacgacc acatgtttac ccgcctcaag cgccgctttg gctaacggga
    11161 aatgggtatc gttgggtgta ggaatgacaa tcaggtctat gttgggatcg ttaaacagat
    11221 gcttcggctc agagacaacc gtaaccgttg gccagtcggc ttttactttt gtttcatcac
    11281 tgctggagat taccgccagt tccagcccgg gcgtgcccgc aatcaggggc gcatggaagg
    11341 ttttgctcgc ataaccatac ccaatcaacc caacacggat gttgtcgctc atgtcattgc
    11401 ctctcattga agtatgatgg ctatttgaca ctatccttta cccacgctca acagtttaat
    11461 aacctgccag caataaggga tgttgtttaa cttaagtcaa aaaaatagcg aattttccaa
    11521 cgacaaaagc taaatatcgc aaaaacctca gtaaaaatct tgctggagct attattgcta
    11581 agtaacattt accccctgaa gttaatggat caatcaagag agatgtgggc tgtaatgaat
    11641 cgtcttattg aattaacagg ttggatcgtt cttgtcgttt cagtcattct tcttggcgtg
    11701 gcgagtcaca ttgacaacta tcagccacct gaacagagtg cttcggtaca acacaagtaa
    11761 gctctgcact tgtggagcga catgctgccc gtccgggtgc atgttttcac ttgtcggata
    11821 ttaaaccagg aatttattat cttgttcgat gttgttggtg attgtcaggg atagtaaagc
    11881 gcgttactct tttcagagca gtttttcatc cctgaatatt tgccatcagc gcattcttat
    11941 tattgtaata acacaagcgt tcacttttgg tgaaatttta tcatcactaa taatacctac
    12001 ccctttggta ttaattattt tatggacctt ttatgactgt tcaggactac ttattaaaat
    12061 ttcgcaaaat cagttcactc gaaagtctgg aaaaactcta cgaccatctt aattacaccc
    12121 tgacggacga tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt cgcgcagagc
    12181 tgctttctgg cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc tggcactatg
    12241 tccaataaag aaagtagcga tcggttataa aagccttata ataacgcccc taaaattaga
    12301 tttgcctgtt tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc acaacgtatt
    12361 ggaggctggt tgcttggccc gttggcctgg cttttagtcg ctctgttaag tacgacgctg
    12421 gcgttgttgc tgtataccgc tgcgttatct tctccgcaaa catttcaaac gctcggcgga
    12481 caagcactga cgacacaaat cttatggggc gtttctttta ttaccgctat cgccatgtgg
    12541 tattacacac tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt tcctaaacac
    12601 tatattatct ggctgcttat ttccgtattg ctggcagtaa aagcattcgc tttttcaccc
    12661 gtggaagatg gcatagccgt tcgccagttg ctgtttactt tgttggcaac cgcattgatc
    12721 gtcccctatt tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc gtaataacct
    12781 tacagttaac ctgttgtcgc ctgctctgga ttaacggata ataggcggct tttttatttc
    12841 aggccgaaaa atgactgact acctgttact gtttgtcgga actgtactgg tcaataactt
    12901 tgtactggtc aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa aactggaaac
    12961 cgcgatgggc atggggctgg caacaacgtt tgtgatgacg ctggcgtcta tttgcgcctg
    13021 gcttatcgat acgtggattt tgatcccact taatctgatt tacctgcgca ccctggcatt
    13081 tattctggtg attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca aaaccagccc
    13141 ggtgctttac cgtctgctgg ggattttttt gccgcttatc accaccaact gtgcagtgct
    13201 cggcgtggcg ttgctgaata tcaatctcgg gcacaatttc ttgcagtctg cgctgtacgg
    13261 tttttccgcc gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca tccgcgaacg
    13321 ccttgctgtg gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt taattaccgc
    13381 aggtcttatg tctctggcct ttatgggctt tagtggtttg gtgaagttgt aatgaatgct
    13441 atctggattg ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc cattctgggt
    13501 tatgcctccc gccgttttgc ggtggaagac gatccggtcg ttgagaaaat tgacgaaatc
    13561 ttaccgcaga gccagtgtgg tcagtgcggt tatcccggct gtcgccccta cgcggaagcc
    13621 atcagctgta acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc tgtgatgcta
    13681 aaaattgccg agttgcttaa tgtcgagccg cagccgctgg atggcgaagc gcaagagata
    13741 acgcctgcgc ggatggtggc ggttattgat gaaaataact gtattggctg cactaaatgt
    13801 attcaggcgt gtccggtaga cgccatcgtt ggcgctaccc gtgccatgca tacggtaatg
    13861 agtgatctct gtacgggctg caatttatgt gttgatccgt gcccgacgca ctgcatctcg
    13921 ttgcaaccgg tcgcagaaac acctgactcc tggaaatggg atctgaacac cattcctgtg
    13981 cgtatcattc ccgtggaaca ccatgcttaa tttattctct gcattcagaa aaaataaaat
    14041 ctgggatttc aacggcggca tccatccacc ggagatgaaa acccagtcca acggtacacc
    14101 cctgcgccag gtccccctgg cgcagcgttt tgttattcca ctgaaacagc atattggcgc
    14161 tgaaggtgag ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc cacttacccg
    14221 tggtcgcggc aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta cggctattgc
    14281 gccccactct acggctcatc cttcagcttt agctgaatta agcgtgatta ttgatgccga
    14341 tggtgaagac tgctggatcc cgcgcgacgg ctgggccgac tatcgctctc gccgtcgcga
    14401 agagttaatc gagcgcattc atcagtttgg tgttgccggg ctgggcggtg cgggattccc
    14461 gacaggcgtt aaattgcagg gtggcggaga taagattgaa acgttgatta tcaacgctgc
    14521 tgagtgcgag ccgtacatta ccgccgatga ccgtttgatg caggattgcg cggctcaggt
    14581 catagagggt attcgcattc ttgcgcatat tctgcagcca cgcgaaattc ttatcggcat
    14641 tgaagataac aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg actctaacga
    14701 tatttctctg cgggtgattc caaccaaata tccttcgggc ggtgctaaac aattaaccta
    14761 cattctgacc gggaagcagg ttccacatgg cgggcgttca tccgatatcg gcgtattaat
    14821 gcaaaacgtc ggcactgctt atgcagtgaa acgtgccgtt attgatggcg agccgattac
    14881 cgagcgtgtt gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg tctgggcacg
    14941 gctggggacg ccagtgcgtc atttattgaa tgatgccgga ttctgcccct ctgccgatca
    15001 aatggtgatt atgggcggcc cgctaatggg ctttaccttg ccatggctgg atgtcccggt
    15061 cgtaaagatt accaactgtc tgttggctcc ctctgccaat gaacttggcg aaccacagga
    15121 agaacaaagc tgcatccggt gtagcgcctg tgctgacgcc tgccctgcgg atcttttgcc
    15181 gcaacagttg tactggttca gcaaaggtca gcaacacgat aaagctacca cgcataacat
    15241 tgctgattgc attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata ttcccctggt
    15301 gcaatatttc cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag aaaagcgcgc
    15361 cgcagaagcc aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg aaaaagcggc
    15421 tcgccttgaa cgacataaga gcgcagccgt tcaacctgca gccaaagata aagatgcgat
    15481 tgctgccgct ctggcgcggg tgaaagagaa acaggcccag gctacacagc ctattgtgat
    15541 taaagcgggc gaacgcccgg ataacagtgc aattattgca gcacgggaag cccgtaaagc
    15601 gcaagccaga gcgaaacagg cagaactgca gcaaactaac gacgcagcaa ccgttgctga
    15661 tccacgtaaa actgccgttg aagcagctat cgcccgcgcc aaagcgcgca agctggaaca
    15721 gcaacaggct aatgcggaac cagaagaaca ggtcgatccg cgcaaagccg ccgtcgaagc
    15781 cgctattgcc cgtgccaaag cgcgcaagct ggaacagcaa caggctaatg cggaaccaga
    15841 agaacaggtt gatccgcgca aagccgccat cgaagccgct attgcccgag ccaaagcgcg
    15901 caaactggaa cagcaacagg ctaatgcgga gccagaagaa caggttgatc cgcgcaaagc
    15961 tgccgtcgaa gcggctattg cacgcgccaa agcacgcaag ctggaacagc aacaggctaa
    16021 tgcggtacct gaagaacagg ttgatccgcg caaagccgcc gtcgaagccg ctattgcccg
    16081 tgccaaagcg cgcaagctgg aacagcaaca gactaacgca gaaccagaag aacaggttga
    16141 tccgcgcaaa gcggcagttg ccgcggctat tgcccgcgct caggccaaaa aagccgccca
    16201 gcaaaaggtt gtaaacgagg actaaatggt attcagaata gctagctccc cttataccca
    16261 taaccagcgc cagacatcgc gcataatgct gttggtgttg ctcgcagccg tgccaggaat
    16321 cgcagcgcaa ctgtggtttt ttggttgggg tactatcgtt cagatcctgt tggcatcggt
    16381 tagtgctctg ttagccgaag ctctcgtact caaactacgc aagcagtcgg tagccgcaac
    16441 gttgaaagat aactcagcat tgctgacagg cttattgctg gcggtaagta ttccccccct
    16501 cgcgccatgg tggatggtcg tgctgggtac ggtgtttgcg gtgatcatcg ctaaacagtt
    16561 gtatggcggt ctggggcaaa acccgtttaa tccggcaatg attggttatg tggtcttact
    16621 gatctccttc ccggtgcaga tgaccagctg gttaccgcca catgaaattg cggtcaacat
    16681 ccctggtttt atcgacgcca tgcaggttat tttcagcgga cataccgcca gtggtggtga
    16741 tatgaacaca ctacgcttag gtattgatgg cattagtcag gcgacaccgc tggatacatt
    16801 taaaacctct gtccgtgccg gtcattcggt tgaacagatt atgcaatatc cgatctacag
    16861 cggtattctg gcgggcgctg gttggcaatg ggtaaatctc gcctggctgg ctggcggcct
    16921 gtggttgcta tggcagaaag cgattcgctg gcatattccc ctcagcttct tagtaacgct
    16981 ggcgttatgc gcaacgttgg gctggttgtt ctcaccagaa acactggcag caccgcaaat
    17041 tcatctgctg tctggtgcga ccatgctcgg cgcattcttt attttgactg acccggttac
    17101 cgcttctacg accaatcgtg gtcgtcttat tttcggcgcg ctggcgggct tattagtctg
    17161 gttgatccgc agtttcggcg gctatcctga cggcgtggct tttgccgtcc tgctggcgaa
    17221 catcacggtt cctctgatcg attactacac gcgtccgcgc gtctacggcc atcgcaaagg
    17281 gtaaaccatg ctgaaaacta tccgaaaaca cggcattacg ttggcgctat ttgcagcggg
    17341 ttcaacaggg ttaactgcgg ccatcaacca gatgaccaaa acgacgattg ctgaacaggc
    17401 cagtctgcaa caaaaggcgt tatttgatca ggtgctgcca gccgaacgct ataacaatgc
    17461 gctggcacag agttgctatc tggtaactgc gccagagtta ggtaaaggtg agcatcgggt
    17521 ttacatcgcc aaacaggatg acaaaccggt agccgccgtt ctggaagcaa ccgcgccaga
    17581 tggctattcc ggtgcgattc agctgctggt gggagccgat tttaacggca cggtacttgg
    17641 cacgcgcgtg acagagcacc acgaaacgcc agggcttggc gataaaatcg aactgcgcct
    17701 ttctgactgg atcacccatt ttgcgggtaa aaaaatcagt ggtgcagatg atgcgcactg
    17761 ggcggtgaag aaagatggtg gtgatttcga ccagttcacc ggcgcgacga ttactccccg
    17821 cgcggtggtt aatgcggtaa aacgcgccgg attgtacgct cagacgttac cggcacaact
    17881 ttctcaactt cctgcctgtg gagaataaag cgtgagcgaa attaaagacg ttattgttca
    17941 ggggttgtgg aaaaacaact ctgcgctggt ccagttgctc ggcctttgtc ctctgttggc
    18001 ggtcacgtcc actgccacta acgctctggg tttaggactt gcgactacgc tggtactgac
    18061 gctgaccaac ctgaccattt cgacgctgcg tcactggacg ccagccgaga tccgcattcc
    18121 catttacgtg atgatcatcg cctcggtggt cagcgctgta cagatgctga tcaacgccta
    18181 cgcctttggc ctgtatcaat cattagggat ttttattccg ctgattgtca ctaactgtat
    18241 cgttgtgggc cgcgctgaag ccttcgccgc caaaaaaggt ccggcgcttt cggcactgga
    18301 cggcttttca attggtatgg gcgcaacctg cgccatgttc gtgctgggtt cactacgcga
    18361 aattatcggc aatggcacat tgtttgacgg tgcagatgcg ctgttaggta gctgggcaaa
    18421 agtattacgc gtggaaattt tccacaccga ctcccctttc ctgctggcga tgctgcctcc
    18481 aggtgcattt attggactgg gactgatgct ggcaggaaaa tacctgattg atgaaagaat
    18541 gaaaaagcgc cgtgctgaag cagctgccga acgtgcattg ccaaacggtg aaacagggaa
    18601 tgtctgatga acaaagcaaa acgcctggag atcctcactc gcctgcgtga gaacaatcct
    18661 catcccacca ccgagcttaa tttcagttcg ccttttgaat tgctgattgc cgtactgctt
    18721 tccgctcagg cgaccgatgt cagtgttaat aaggcgacgg cgaaactcta cccggtggcg
    18781 aatacgcctg cagcgatgct tgaactgggc gttgaagggg tgaaaaccta tatcaaaacg
    18841 attgggcttt ataacagcaa agcagaaaat atcatcaaaa cctgccgtat cttgctggag
    18901 cagcataatg gcgaggttcc ggaagatcgt gctgcgcttg aagccctgcc cggcgtaggt
    18961 cgtaaaacag ccaacgtcgt attaaacact gcattcggct ggccgactat tgctgtcgac
    19021 acgcacattt tccgcgtttg taatcgtact caatttgcgc cggggaaaaa cgtcgaacag
    19081 gtagaagaaa agctactgaa agtggttccg gcagagttta aagtcaactg ccaccattgg
    19141 ttgatcctgc acgggcgtta tacctgcatt gcccgcaagc cccgctgtgg ctcttgtatt
    19201 attgaagatc tttgtgaata caaagagaaa gttgacatct gaagaaaagg ggtaacaccg
    19261 attaccccat tgataacctt tctttatcct cttttaaaac atctttttaa cgtcaatgat
    19321 gccattgctt agcgttatca tcaggtaatc cgtttgcgga taaccaaaaa tgcaggttaa
    19381 ttgttttttt aatagcgaaa ttttctattc attcgtgatc aagatcacga tgctgtggca
    19441 atgtaaaaac attgcaagaa tcaggttaaa atcatttaaa tttacacacg caacaaatat
    19501 tgacctacaa aacattacac tggctatttt tcagaaactg ggctatctca ctagtaaaaa
    19561 cgctaaatag cagaacatat cgccgttaag ccatcacccc cctctcagtg cagtgaaaaa
    19621 atctgccgtt acgttttttg aaaaatttaa cgctggataa catttcccgg aatggttgaa
    19681 ttccccgcct cagttatatg taacagatta ttacaaagga cttgtctgaa agtgcaagat
    19741 agtgaacatt acctgccgtt tcccctccca ctataacaat tgcgcgtatg ttttttagac
    19801 ataacgcgag aaagcacccc cgttaatatg ggatgtaaaa aaagaggtaa aagtgtccac
    19861 tgcaaaccaa aaaccaactg aaagcgtcag tttgaacgct ttcaaacaac cgaaggcgtt
    19921 ctatctcatc ttctcgattg agttatggga acgttttggt tattacggcc tacaaggaat
    19981 tatggctgtt tacctggtta aacaactggg tatgtctgaa gcggattcaa tcaccctttt
    20041 ctcttccttt agtgccctgg tttatggtct ggtcgctatc ggcggctggt taggtgacaa
    20101 ggtactgggt actaaacgcg taattatgct cggcgctatt gtgctggcga ttggttatgc
    20161 tctggttgcc tggtctggtc acgacgccgg tatcgtttat atgggtatgg cggctattgc
    20221 ggtcggtaac ggcctgttta aagctaaccc gtcttctctg ctttctacat gctatgagaa
    20281 aaacgacccg cgtctggacg gtgcattcac catgtactac atgtccgtca acatcggctc
    20341 tttcttctct atgattgcta cgccgtggct ggccgcgaaa tacggctgga gtgttgcgtt
    20401 tgcgttgagc gttgtaggcc tgctgatcac tatcgttaac ttcgccttct gccaacgctg
    20461 ggttaaacag tacggttcaa aaccagactt cgagcctatc aactaccgta acctgctgct
    20521 gaccattatt ggtgttgtgg cactgatcgc tatcgccacc tggctgctgc acaatcagga
    20581 agttgcgcgt atggcgctgg gcgttgttgc cttcggtatc gtggttatct tcggtaaaga
    20641 agccttcgcg atgaaaggtg ctgcgcgtcg taaaatgatc gttgccttca tcctgatgct
    20701 cgaagccatt atcttcttcg tgctgtacag ccagatgcca acgtcactga acttctttgc
    20761 gattcgtaac gttgagcact ccattctggg tctggccgta gaacctgagc agtatcaggc
    20821 actgaacccg ttctggatca tcatcggtag tccgattctg gccgctatct ataacaagat
    20881 gggcgatacc ctgccgatgc caaccaagtt tgcaatcggc atggtgatgt gttctggtgc
    20941 gttcctgatt ctgccgctgg gtgcgaaatt cgcgtctgac gctggtatcg tgtctgtaag
    21001 ctggctggtc gcaagctatg gcctgcagag catcggggaa ctgatgatct ctggtctggg
    21061 tctggcaatg gttgcacaac tcgttccgca gcgtctgatg ggcttcatta tgggtagctg
    21121 gttcctgacc actgccggtg caaacctgat tggtggttat gttgcgggta tgatggctgt
    21181 gccggataac gttaccgacc cgctgatgtc actggaagtt tatggtcgcg tattcttgca
    21241 gattggtgtc gctactgcca ttattgcagt actgatgctg ctgaccgcgc ctaaactgca
    21301 ccgtatgacg caggatgacg ctgcagacaa agcggcgaaa gctgctgtag cgtaaaattc
    21361 agggaaactc ttttacaagt cgctaacttt tcgttagcgg cttttttttg ttcagcaacg
    21421 cactaccata ctttaaacca cagacaaaag gagttaccga tgaaattgtt ctacaaaccg
    21481 ggtgcctgct ctctcgcttc ccatatcacc ctgcgtgaga gcggaaagga ttttaccctc
    21541 gtcagtgtgg atttaatgaa aaaacgtctc gaaaacggtg acgattactt tgccgttaac
    21601 cctaaggggc aggtgcctgc attgctgctg gatgacggta ctttgctgac ggaaggcgta
    21661 gcgattatgc agtatcttgc cgacagcgtc cccgaccgcc agttgctggc accggtaaac
    21721 agtatttccc gctataaaac catcgaatgg ctgaattaca tcgccaccga gctgcataaa
    21781 ggtttcacac ctctgtttcg ccctgataca ccggaggagt acaaaccgac agttcgcgcg
    21841 cagctggaga agaagctgca atatgtgaac gaggcactga aggatgagca ctggatctgc
    21901 gggcaaagat ttacaattgc tgatgcctat ctgtttacgg ttctgcgctg ggcatacgcg
    21961 gtgaaactga atctggaagg gttagagcac attgcagcat ttatgcaacg tatggctgaa
    22021 cgtccggaag tacaagacgc gctgtcagcg gaaggcttaa agtaatatcc atcggcccac
    22081 aggtcgttgt gggccgaaat gagatatttc agagctttgt tgcgctgaag taatgttctg
    22141 gtttggcaat acgatcctga gcagccacca cttgcagctc atattcctgc attgctttgg
    22201 tggtcaccat gatttcgtag actgcagcgg tcacatgttc cagcgcctcc tgcagcgttg
    22261 ccccctgaag cagtttcacc agcagtaaac cgctcgtcac atcaccaaca cctaccggct
    22321 ggcgcatacc aaaatccacc agcggacggc tgatatgcca ggcttcatcg gcggtgacca
    22381 gcagcatttc aaaacggtca cggctgtagc cagctcgcgc caggtgttta accaacacaa
    22441 tttgtggccc ttgcgcaatg agttcgcgcg ctgccagaac cgcttcttcg acgttattta
    22501 ccgcatgctc acagagtatt tccagctcaa ccagattcgg cgcaatgata tcgctggcag
    22561 gcaaaccgtg ccgcacatga aactctgcga cacccggtgc aacgatacag cctttttccg
    22621 gatgacccat taccggatcg caaaaatatt tcgcctgcgg attcgcggct ttcacctgac
    22681 ggacgatacc gaggatatgt tcaccctgct ccgccgatcc cagatagcca cttaacacgg
    22741 catcacaggt gtgtaattta tcaatggcgg caatgccttg cacaatttcg gttaaatggc
    22801 tgggcggcat cacgcagcca gtccatttgc cgtactgggt gtgattagaa aattgaacgg
    22861 tgttcagcgg ccagacgttc gcgcccaagc ggcgcatagg aaactctgcc gcactgttac
    22921 ccgcatgacc ataaacaacg tgagactgga tagcgagaat atttttcatc atgttccctg
    22981 tataaaaacc agggggagtg atttctcact ccccctttcc acttaatgca ttatttccag
    23041 caaatcagac agtaattctt tttgccgcga cgcagtaagg taaaacgacc aaacagacga
    23101 tcttcttctt taaagaagta ttcaggatcg gactgttttt caccgttaat ggtgatggca
    23161 ttggaggcga tagttttacg cgcctgacca cgggaaggtt gcagttcaga atcgaccagc
    23221 gcctgcatca ggtctgcgcc cttttccatc tcaaccatcg gtacgccgtc ctgcgccagc
    23281 tgttcgaagt ccgcttcact cagcgcactc aaagaaccgc tgaacaggca ttcggtaata
    23341 cgttttgccg cctgtaaacc ttcttcaccg tgaaccagac gagtcacctg ctccgccagt
    23401 acatactggg cgcgcggtgc tttagcgctg tttttatctt cttcttccag ggcgttgatc
    23461 tcttcaatgc tcataaaggt gaagaacttc aggaagcggt aaacgtcggc atccgcagtg
    23521 ttgatccaga actggtagaa tttgtacggg ctggttttct tcggatccaa ccagactgcg
    23581 ccgccttcag ttttaccaaa tttggtgcca tctgctttag tgatcagcgg aacggtcagg
    23641 ccaaacacct gattctgatg cagacgacgg gtcaggtcga taccagaagt gatgttaccc
    23701 cactggtcag aaccaccaat ttgcagcacc acaccgtact gtttgttcag acaggcgaag
    23761 tcataaccct gcaacaggtt gtaggaaaac tcagtgaacg aaatcccctt atcttcacgg
    23821 ttgagacgct gcttaaccgc ttctttgttg atcatctggt taacggagaa gtgtttgcca
    23881 atatcgcgca ggaaggtcag cacattcata ttgccgaacc agtcatagtt gttcgccgcg
    23941 atagcagagt tttctccaca gtcgaaatcg aggaacgggg caacctgctt acggattttg
    24001 tccacccact cctgaacagt ttcttcggtg ttcagcttac gctcggcagc tttgaagctc
    24061 gggtcgccaa tcagacccgt cgcgccgcct accagcgcaa ccggcttgtg gcccgcctgc
    24121 tggaagcgtt tcaggcataa caatggaaca agatgcccca aatgcaagct gtcagcggta
    24181 ggatcgaagc cgcaatagag cgcgatcggg ccttgcgcca gtcgctctgc taacgcttcc
    24241 tcgtccgtca cctgggctac cagcccccgc tcttgcaatt gtttaatcaa gttactgctt
    24301 gccatcaaaa tctccatgta tataacgact gcacctttgc cggtacacga cttttcgcca
    24361 gatgcgaaag agacatagaa taaagtgcca gaatcaggag taccagcgat taaagcaaga
    24421 tttttgcatc ttttcagggt gcaagacgat caatcttcca cgcatcattt tctcgctggt
    24481 acaaaaagcg gtcatgcagg cgatgctcac cgccctgcca gaactcaatc tgttcaaggc
    24541 tgacgcgaaa accgccccaa aagctcggca atggcacttc gccctgttga aacttctgct
    24601 tcagctccag gaatttactt tcaaggatac cgcgggcaga aatgcgactg gactgcttcg
    24661 aaacccatgc accaatctgg ctatcacgcg ggcggctatg aaaatatttc atcacttcga
    24721 gagtcgaaag tcgttctgct ttaccgatca ccatcacctg gcgttcaaga gtatgccacg
    24781 ggaacagcag gctaacgcgc ggattatttt caatttgatg tgctttacgg ctaccgaggt
    24841 tggtgtaaaa caccatgcct ttttcgtcgt aatgtttgag taaaacgatg cgttgataag
    24901 gctgagcatg ttcatccacg gtagcgacca ccatcgcggt agggtctgcc agtttggctt
    24961 cacacgcctg agagagccaa cgttcaaaaa gggttaatgg atcggcggga agatcgcgac
    25021 ggcgtaaccc gcctttggtg tattcacggc gcagatgcgc gatttgctgc aattcgtcgt
    25081 tatcagacat ggttttcttt atggattgtc agtgggtgac gctattgtgc gccgcccatg
    25141 gaaaaatctc aacgctgtgg attttgtaac tgacagttat tcaatacgat gcggtcgcgt
    25201 ttatagacag tcgcttcatc gcctttcgac cagaaaacat agattccgtc agtgtaacgt
    25261 gcaccagacg ctgaaatgcc ctgtttgaga tgcagtagtt gattatcgta aacaaaactg
    25321 acttcctggc gcggattatt cagtttgacc gtcaacggtt tttcatcaca ctggtattcc
    25381 agcgtagcgg tttgcatgtg ctcaaccagt tgattgaacg cgctacagcc ggtgagaagc
    25441 accggcaaac agatcagtaa cagttttttc atagacattt cccgaagact ttcctggtct
    25501 ggagggcaat acgccctccc taacgttcca agtgtaacgg cagacgcggt aagaaaaatt
    25561 cagttaactc tgatatcacg ggttagcagg gaaaatagcc cccagtaccg tctcctggct
    25621 tgcgccagtg acggaaggca gatttcctgg taatcccgcc agcgtccgcc aggcaagcca
    25681 ggcgaaagcc aatgcttcca tgtcatcgcc actaatgcca acggcatcgg tggtggtgac
    25741 ttctgtgcct ggcagtaatg ccgccagacg cgccatgagt agcggattac gactacctcc
    25801 accacatacc atcaatcgtt cgcagccacc gctcaacaaa acttgttcag aaatggtcac
    25861 gacggtgagt tctgccagtg tggcctgaac atcacgggga tcaacacccg gaaaatggcg
    25921 taaatggcgc tccagccagc cgtagttaaa gtattcgcgt ccggtgcttt tcggtgcagg
    25981 ttgcgagaaa tatgggtcgc tgagcatatt ttgcagcagt gggagaataa ctttacccgc
    26041 ccgtgcccac tcggcatctt tatcgtaagg tttaccgacc tgacgccaga tccaggcatc
    26101 catcagcatg ttaccaggac cggtatcgta gccgccaacc ggctgcccag gaatgagcag
    26161 tgacagattg gcgatgccgc caatattgag caccattcgt cgctcggttg ggtgagccag
    26221 caaggcatga tggaacgcag gtaccagcgg tgcgccttgt cctcccaagg caatatcgcg
    26281 acggcgaaaa tcaccgacaa ccgtaattcc ggtgcgtgcc acaatttgat tgttatcgcc
    26341 aatctgtaaa gtgtgtggtg ccacgcccgt cggttcatgc cagacggttt gaccgtgaca
    26401 gccgatcgca actatatctc tcgcctgcag gttttgttcc ttaagcaagg cattgaccgc
    26461 atcagcaaaa agtcgcccga gttgagtatc aagctgtcca aactgcgaaa gtgtaagctg
    26521 ctggccctgg caaatatcca gtacagcctg tttcagagac accgggatcg gccagctcaa
    26581 gcttgccagc tgtgcgaccc ggtgttcatc tattgtcgcc aacacaacat caacaccatc
    26641 aaggctggtg cctgacataa cgccaataaa gcggcccgat ttcatagttc atcctttttc
    26701 aatctgacgt ttgcgcacca ctcaaacata aacttttcgt gaataccatg cggaatgacc
    26761 gatttttacc gttggtagta aaacattatc ttcaaatcaa taatcatcat gaatgttttg
    26821 tttataattg gttgatccta ctttcattat gatttgctca tatttggtag aacatgtaac
    26881 catggattca catatgccat atactttgac catgagggat gcttgcgtgg cgtttcatgg
    26941 tgaacaggag atttttcaat gattaaacgc gtattggttg tttcaatggt aggtctgtct
    27001 cttgtcggtt gtgttaataa cgacaccctg tcaggggatg tttataccgc ttctgaagcg
    27061 aaacaagtac agaatgtcag ctatggcacc atcgttaacg tacgtccggt acagattcag
    27121 ggcggtgatg attccaacgt tatcggtgca attggcggtg ctgttcttgg tggtttcctg
    27181 gggaatactg ttggtggcgg aaccgggcgt tctctggcta ctgcagcagg cgctgttgca
    27241 ggtggcgtag ctggtcaggg cgtacagagt gcaatgaaca aaacgcaggg tgtcgagctg
    27301 gaaattcgta aagacgatgg taataccatc atggtggtac agaaacaagg caacactcgt
    27361 ttctctccgg gccaacgtgt cgtactggcc agcaatggca gtcaggtgac cgtttctccg
    27421 cgctaaataa gtttgcgtgt ggtcaggtta ctgaccacac gcccccttca tttcaccctt
    27481 tggcctgtaa ctcaatgata ttatgctcaa gttttgcgat gagcgtaatc agttgctcca
    27541 gttcctctgc ggagatgcca tgtaatattt ccgcgcgggt tttgttaata acagcttcca
    27601 tttcgctgat cagtggctct gccttttccg tcagtttaat acgtttagcc cgacgatcgc
    27661 tggcacaagt ttgacgcgaa attaaccctt tttcttccag ttggtccaga gtacggacca
    27721 gtgatggctg ctcgatgcca atcgcttttg ccagttgaat ttgcgactgg tctggaggta
    27781 actgatggat attgtgtaac gtaacccaat gggtttgtgt taactccagc ggtttcaggc
    27841 gatggtctat cagagcacgc catatgcgca ccaaccgtgc cagatcagaa cctagtggcg
    27901 attccaattt catctcctta taattagctt gctaagatat tatgcggctt ttagaatagt
    27961 gtgcagcaat tgtattgcta aaacaaatgt attgctgcat ttggttaccg tcagacatat
    28021 ttttcagaaa ttgcgcgtaa atttttcgca cttaaagaat atttattaat ctaacgcaat
    28081 atattcggtc gtaaaggaat ctactttgtg aagtttatgc tcaatgcaac aggattgccc
    28141 ttacaagatc tggtgttcgg tgcgtccgtc tattttcctc cgtttttcaa agccttcgcg
    28201 tttggattcg tcatctggct tgtcgtacac cgcctgcttc gtggctggat ctacgccggt
    28261 gacatctggc atcccttgtt aatggattta tcgctgtttg cgatttgcgt ttgccttgct
    28321 ctggcaatac tgattgcgtg gtaactatgt caattaaaac aattaagtat ttctcaacaa
    28381 tcattgtagc gatagttgcg gttcttgccg gatggtggct gtggaattat tacatgcaat
    28441 ctccgtggac acgcgacggg aaaatacgcg cagaaaaggt ttctatcacc ccacaggtgt
    28501 cgggacgtat tgttgagctg aatataaaag acaaccaact ggttaacgcg ggggatcttc
    28561 tgctcacgat cgataaaacg ccctttcaga tcgccgagct gaacgcacag gctcagctgg
    28621 cgaaagcgca atctgacctc gcaaaagcca ataacgaagc gaatcgtcgt cgtcatctct
    28681 cacaaaattt tatttctgcc gaagaactcg ataccgctaa cctcaatgtt aaagcgatgc
    28741 aggccagtgt taatgccgca caggccacgc tgaaacagac acaatggcaa ctggcgcaaa
    28801 cggaaattcg cgccccggtg agtggttggg tgactaacct cactacccgc atcggcgatt
    28861 acgctgacac cgggaaaccg ctgtttgccc ttgtcgacag ccactcgttt tacgtcattg
    28921 gttattttga agaaaccaag ttgcgccata tccgcgaagg tgcaccagca caaattaccc
    28981 tctatagtga taacaaaacg ttacggggtc acgtttccag tatcggtcgg gcgatttatg
    29041 atcaaagcgt tgaaagtgac tcaagcctta ttccggatgt gaaacctaac gtcccctggg
    29101 ttcgtctcgc ccaacgcgtt cccgtccgct ttgcgctgga taaagttcca ggcgatgtca
    29161 cgctggtgtc gggcaccacc tgtagcattg ccgtaggtca ataatgaacg catcgtcatg
    29221 gtccttgcgc aatttgccct ggttcaaggc cacgctggcg caatggcgtt atgcgttacg
    29281 caataccatt gccatgtgtc tggcgctgac ggttgcctat tatttgaatc tggatgaacc
    29341 ctattgggcg atgacctcgg ctgcagtggt tagctttccc accgttggcg gtgtcatcag
    29401 caaaagcctc ggatgcatcg ctggcagttt actgggagcc attgcggcac tgcttcttgc
    29461 cgggcatacg ctcaatgaac cgtggttttt tctattgagc atgtcggcgt ggcttggctt
    29521 ttgtacctgg gcctgtgcgc acttcacgaa taacgtcgcg tatgcatttc aactggcggg
    29581 ctacacggct gccatcatcg cctttccgat ggttaatatt actgaggcca gccagctgtg
    29641 ggatatcgct caggcgcgcg tttgcgaggt gatagtcggt attttgtgcg gcggcatgat
    29701 gatgatgatc ctgcctagca gttccgatgc tacagccctt ttaaccgcat tgaaaaacat
    29761 gcacgcccga ttactggaac atgccagttt actctggcag cctgaaacaa acgatgccat
    29821 tcgtgcagca catgaggggg tgattgggca gatactgacc atgaatttgc tgcgtatcca
    29881 ggctttctgg agccactatc gttttcgcca gcaaaacgcg cgccttaatg cgctactcca
    29941 ccagcaatta cgtatgacca gtgtcatctc cagcctgcga cgtatgttgc tcaactggcc
    30001 ctcaccgcca ggtgccacgc gagaaattct cgaacaattg ctgacggcgc tcgccagttc
    30061 gcaaacagat gcttacaccg tcgcacgtat tatcgccccg ctacgcccga ccaacgtcgc
    30121 cgactaccgg cacgtcgcct tctggcagcg actacgttat ttttgccgcc tttatctgca
    30181 aagtagtcag gaattacatc gtctgcaaag cggtgtagat gatcatgcca gactcgcacg
    30241 gacatccgct ctggctcgtc ataccgacaa cgccgaagct atgtggagcg ggctgcgtac
    30301 attttgtacg ttgatgatga ttggcgcatg gagtattgca tcgcaatggg atgccggtgc
    30361 caatgcatta acgctggcag caattagctg cgtactctac tccgccgtcg cagcaccgtt
    30421 taagtcgttg tcactgctga tgcgcacgct ggtgttactt tcgctattca gctttgtggt
    30481 caaatttggc ctgatggtcc agattagcga tctgtggcaa tttttactgt ttctctttcc
    30541 actgctggcg acaatgcagc ttcttaaatt gcagatgccc aaatttgccg cactgtgggg
    30601 gcaactgatt gtttttatgg gtttgtttat cgctgtcact aatcccccgg tgtatgattt
    30661 tgctgatttt cttaacgata atctggcaaa aatcgttggc gttgcgctgg cttggctagc
    30721 gttcgccatt ctgcgtccag gatcggatgc tcgtaaaagc cgccgccata ttcgcgcgct
    30781 gcgccgggat tttgtcgatc agctaagccg ccatccaaca ctgagtgaaa gcgaatttga
    30841 atcgctcact tatcatcacg tcagtcagtt gagtaacagc caggatgcgc tggctcgccg
    30901 ttggttatta cgctggggtg tagtgctgct gaactgttct catgttgtct ggcaattgcg
    30961 cgactgggaa tcgcgttccg atccgttatc gcgagtacgg gataactgta tttcactgtt
    31021 gcggggagtg atgagtgagc gtggcgttca gcaaaaatca ctggcggcca cacttgaaga
    31081 attacagcgg atttgcgaca gccttgcccg tcatcatcaa cctgccgccc gtgagctggc
    31141 ggcaattgtc tggcggctgt actgctcgct ttcgcaactt gagcaagcac caccgcaagg
    31201 tacgctggcc tcttaattac ttaattacac cacaggcata gcgttcaccg ccaccgccca
    31261 gcggtttagg ttgatcggac atattatcgc cgccaacgtg gaccatcagc gctttgtctt
    31321 tgatttcatc cagtgatttc agacgaggcg cgatgacggc atcggtagct ttgccgtcat
    31381 tattgacgac cagtgcaggc agatcgccta aatgcccggc accttctggc ccttcatgtt
    31441 taccggtatt ttgtggatca agatgcccgc ctgcggattc cgcggcgctg gctttgccat
    31501 ctttggtggc tggctggcag cttcctttgg catgaatatg gaagccatgt tcaccggggg
    31561 gtaatgcttt cagatcgggc gaaaactcca gacctttatc ggtttcagta atggtgacgc
    31621 taccaattga ctgccctacc ccttgcgacg tgacgaggtt catctcgact ttttcactgg
    31681 cagcttgtgc gccggttgca acaaccagcg ccagaatagc cagactaaaa cgtttcatag
    31741 gacctccgtt cattgtgcat tcctgaataa gtgtaaacca gtgacacatt tttgaacggc
    31801 ggctattcct aaaagtgctt agggtacgtc gtaccccagt gccgctttac ggatacgaaa
    31861 ccattgttga cgggtcattt tcagtgtttc tgcttcgaca gctgcccgta cgcgctcaat
    31921 tttacctgaa ccgataattg gcagcggctg cgatggtaaa cgtaataccc aggcgtaaac
    31981 cacctgttca atcgagcccg cgtttaactc ctctgccacc acagccagtt catcacgcag
    32041 cggctggaaa taatcatcat taaacagacg accaccacca aggcaggacc acgccatcgg
    32101 acgaacacgc agttgttgta gttggtcgag cgtgccatcc agcagtaacg gctgatgcac
    32161 cggggatatt tccacctgat tagtggcaag ggtaaacggc agacgtgatt gcaacagggc
    32221 aaattgcgca ggcgtaaagt tcgatacgcc aaaatgacgc actttgccgc tctgatgcag
    32281 atgtttgaac gcgtccgcca cttcatcggc atccattaac gggtctggtc ggtggattaa
    32341 cagcagatcc agatgatcgg tcgcgagatt aattagcgac tgttcggcgc tcttaatgat
    32401 gtgatcacgg tcagtgatgt aatgaccaat gacgttttct tcacgcgcgg tcgtcgcgat
    32461 accgcattta ctgacgattt ccatccgttc acgcaggtga ggtgccagtt tcagtgcctc
    32521 gccaaacgcc gcttcgcact gatagccacc ataaatatca gcatggtcca cggtggtcac
    32581 gccgagatcc agatgctctt caataaaact gaccagctgg cgggcggaca tattccagtc
    32641 catcaatcgc cagtagccca tcacaaaacg ggaaaactcc gggccttgcg gcgcaatagt
    32701 aatacgctga accataatcg cttcctctta tcagatatga gaggagtata cgcaagatta
    32761 ggttcaaaag agtgatggtt gctccggttc gtctgatgac gctggcttat ttgcgcgtaa
    32821 tttgcgcatt aatcgctgcc gacaaaggcg cagcacctct tgtttttcgc catcgctcat
    32881 tttattccag ttaaaacgct catcccgact acgaaaacag ccgcgacaaa acccgcgttc
    32941 gtcagactgg caaattcccc ggcacgggct ctggacggga aagaactcta attgctccgc
    33001 cacttcgccc tcctcagata agattattac cattattgaa gctgttaatg tccaaagtag
    33061 caactttgct tgcactagac cgactggtct actacactcc aacgcatgaa caaacacacc
    33121 gaacatgata ctcgcgaaca tctcctggcg acgggcgagc aactttgcct gcaacgtgga
    33181 ttcaccggga tggggctaag cgaattacta aaaaccgctg aagtgccgaa agggtccttc
    33241 tatcactact ttcgctctaa agaagcgttt ggcgttgcca tgcttgagcg tcattacgcc
    33301 gcatatcacc agcgactgac tgagttgctg caatccggcg aaggtaacta ccgcgaccgc
    33361 atactggctt attaccagca aacactgaac cagttttgcc aacatggaac catcagtggt
    33421 tgcctgacag taaaactctc tgccgaagtg tgcgatctgt cagaagatat gcgcagcgcg
    33481 atggataaag gtgctcgcgg cgtgatcgcc ctgctctcgc aggctctgga aaatggccgt
    33541 gagaaccatt gtttaacctt ttgtggcgaa ccgctacaac aggcacaagt gctttacgca
    33601 ctatggcttg gcgcgaatct gcaggccaaa atttcgcgca gtttcgagcc actggaaaac
    33661 gcgctggccc atgtaaaaaa cattattgcg acgcctgccg tttagcaggc attttttatc
    33721 accagacgac cgggagcctt tatgtcatct gaaaaactgt attccccact gaaagtgggc
    33781 gcgatcacgg cggcaaaccg tatttttatg gcaccgctga cgcgtctgcg cagtattgaa
    33841 ccgggtgaca ttcctacccc gttgatggcg gaatactatc gccaacgtgc cagtgccggt
    33901 ttgattatta gtgaagccac gcaaatttct gcccaggcaa aaggatatgc aggtgcgcct
    33961 ggcatccata gtccggagca aattgccgca tggaaaaaaa tcaccgctgg cgttcatgct
    34021 gaaaatggtc atatggccct gcagctgtgg cacaccggac gcatttctca cgccagcctg
    34081 caacctggcg gtcaggcacc ggtagcgcct tcagcactta gcgcgggaac acgtacttct
    34141 ctgcgcgatg aaaatggtca ggcgatccgc gttgaaacat ccatgccgcg tgcgcttgaa
    34201 ctggaagaga ttccgggtat cgtcaatgat ttccgtcagg ccattgctaa cgcgcgtgaa
    34261 gccggttttg atctggtaga gctccactct gctcacggtt atttgctgca tcagttcctt
    34321 tccccttctt caaaccatcg taccgatcag tacggcggca gcgtggaaaa tcgcgcacgt
    34381 ttggtactgg aagtggtcga tgccgggatt aaagaatggg gtgccgatcg cattggcatt
    34441 cgcgtttcgc caatcggtac tttccagaac acagataacg gcccgaatga agaagccgat
    34501 gcactgtatc tgattgaaca actgggtaaa cgcggcattg cttatctgca tatgtcagaa
    34561 ccagattggg cggggggtga accgtatact gatgcgttcc gcgaaaaagt acgcgcccgt
    34621 ttccacggtc cgattatcgg cgcaggtgca tacacagtag aaaaagctga aacgctgatc
    34681 ggcaaagggt taattgatgc ggtggcattt ggtcgtgact ggattgcgaa cccggatctg
    34741 gtcgcccgct tgcagcgcaa agctgagctt aacccacagc gtgccgaaag tttctacggt
    34801 ggcggcgcgg aaggctatac cgattacccg acgttgtaat ccaacattgc gagcggcgta
    34861 aagccgccgc tatactaaaa caacattttg aatctgttag ccattttgag gataaaaaga
    34921 tgcgtcttct tcataccatg ctgcgcgttg gcgatttgca acgctccatc gatttttata
    34981 ccaaagtgct gggcatgaaa ctgctgcgta ccagcgaaaa cccggaatac aaatactcac
    35041 tggcgtttgt tggctacggc ccggaaaccg aagaagcggt gattgaactg acctacaact
    35101 ggggcgtgga taaatacgaa ctcggcactg cttatggtca catcgcgctt agcgtagata
    35161 acgccgctga agcgtgcgaa aaaatccgtc aaaacggggg taacgtgacc cgtgaagcgg
    35221 gtccggtaaa aggcggtact acggttatcg cgtttgtgga agatccggac ggttacaaaa
    35281 ttgagttaat cgaagagaaa gacgccggtc gcggtctggg caactaatct cctgccgggc
    35341 gtgaactcat cgcgcccgca tctttactgc atcgacaagt aatatttgtc ataatgcgcg
    35401 ctgcaattta tccgtattaa gagaatcaga tgtccgataa cgctcaactt accggtctgt
    35461 gcgaccgttt tcgtggtttt tatcctgttg tgatcgatgt tgaaacagcc ggatttaacg
    35521 ccaaaaccga tgcgctgctt gagattgccg ccatcaccct gaaaatggat gaacaaggct
    35581 ggctgatgcc ggacaccaca ttacatttcc acgtcgaacc atttgtcggc gcaaatttgc
    35641 aaccagaagc cctcgccttc aacggcattg acccgaacga tcccgatcgc ggcgcggtca
    35701 gcgaatacga ggcgctgcac gaaattttta aagttgtacg taaaggtatt aaagcgagcg
    35761 gctgtaaccg cgccattatg gtggcgcaca atgccaattt tgatcacagc tttatgatgg
    35821 ccgccgcaga acgcgcctcg ctgaaacgta acccgttcca ccctttcgcc acttttgaca
    35881 ctgctgcact ggccgggctg gcactcggac aaaccgtatt gtcaaaggct tgccagaccg
    35941 ctggcatgga cttcgacagc acccaggcgc actccgcgct gtacgacacc gaacgcactg
    36001 ctgtgctgtt ttgtgaaatc gtcaaccgct ggaaacgtct gggaggctgg ccgctacctg
    36061 ccgccgaaga ggtgtaatcg agtcgatgcc tgatgacatg caatgattca ggcatctata
    36121 gtgaggctat tccacgcatc ctgcatgata ttcacgggga atagcgttaa tggcagataa
    36181 tccagaccct tcatcgctcc tgccggacgt gttttcaccg gcgacccgcg actggtttct
    36241 tcgcgccttt aaacagccga ccgctgtcca gtcgcaaacc tggcatgtgg cggcgcgagg
    36301 cgaacatgcg ctggtgattg caccgactgg ctccgggaaa acgctggcag catttctcta
    36361 cgccctcgat cggctcttcc gcgaaggcgg cgaagatacc cgcgaggcgc ataagcgtaa
    36421 aacctcacgc atcctctata tttcaccgat aaaagccctg ggcaccgacg ttcagcgcaa
    36481 cttgcagatc ccgttgaagg gtattgccga tgaacggcgg cggcgcggcg aaacggaagt
    36541 caatcttcgc gtagggatcc gtactggcga tacgcctgca caggaacgca gcaaactcac
    36601 ccgtaatccg ccggatattc tgatcaccac acccgaatca ctctatctga tgctgacctc
    36661 ccgcgcgcgc gaaacgctac gcggcgtcga aacggtaatt attgatgaag tccacgcggt
    36721 agcgggcagt aaacgtggtg cgcatctggc gttaagtctg gagcggctcg atgcgctgct
    36781 ccacacctca gcacagcgaa ttggcctttc tgccactgtg cgctcagcca gcgatgtggc
    36841 agaatttctt ggtggcgatc gcccggttac ggtagtcaac ccgcccgcaa tgcgccatcc
    36901 gcagatacga attgtcgtac cggtcgccaa tatggatgat gtctcatcgg tcgccagcgg
    36961 caccggcgaa gacagccatg ccggtcggga aggctccatc tggccatata ttgaaacggg
    37021 tatccttgat gaagtgttgc gccatcgctc gaccattgtc tttactaatt cacgcgggct
    37081 ggcggaaaaa ctgacggcac gattaaatga gctttacgcc gcacgcttac agcgttcccc
    37141 gtctatcgcc gttgatgcgg cccatttcga gtcgacctcc ggcgcaacct ctaaccgtgt
    37201 acaaagtagc gacgttttta ttgcccgctc acaccacggc tccgtctcta aagaacaacg
    37261 agcaatcacc gaacaggcgc tgaaatcggg tgaattacgc tgcgtgatcg caacctccag
    37321 tcttgaactg gggattgata tgggcgcggt ggatctggtg attcaggtgg caacgccgct
    37381 ttctgttgcc agtgggttac aacgcattgg tcgcgccgga catcaggttg gcggtgtatc
    37441 taaagggctg tttttccccc gtacccggcg tgatttagtc gattccgcag tcattgtaga
    37501 gtgtatgttc gcaggcaggc tggaaaacct gacaccaccg cataatcctc tcgacgtcct
    37561 tgcgcagcaa accgttgccg ccgcggcgat ggatgcatta caggtagacg aatggtactc
    37621 ccgcgtacgc cgtgccgcac cgtggaaaga tctgccaaga cgtgtttttg acgccacgct
    37681 ggatatgctt tccgggcgct atccctctgg cgatttttct gcttttcgcc ccaaactggt
    37741 ctggaacacg gagaccggga tattgaccgc ccgacctggc gctcaattgt tggcggttac
    37801 cagcggcggc accattccgg atcgtggcat gtatagcgtg ttattacccg aaggtgaaga
    37861 aaaggccggt tcgcggcggg tgggtgaact ggatgaggag atggtatatg agtcgcgggt
    37921 gaacgacatt atcactctcg gcgctacctc atggcggatc cagcaaatca cccgcgatca
    37981 ggtgattgtg actcctgctc cgggtcgttc tgcccggctc cccttctggc gtggtgaagg
    38041 taacggacgt ccggctgaat taggcgagat gatcggcgat tttcttcatt tgctggcgga
    38101 tggcgcgttc ttttccggga ctattccccc gtggctggca gaagaaaata cgatcgccaa
    38161 tattcagggg ttgattgacg agcaacgcaa cgcgacgggc atcgttccgg ggagtcgcca
    38221 tctggtcctc gaacggtgcc gtgatgaaat tggcgactgg cgtattattt tgcactctcc
    38281 ctatggaaga cgggtgcatg aaccctgggc gctggcgatt gccgggcgaa tacatgccct
    38341 atggggcgct gacgcgtctg tggtcgccag tgatgacggc attgttgcgc gcattcctga
    38401 cactgatggt aaattgcccg acgccgcgat ttttttgttt gaaccagaaa agttgctgca
    38461 aattgtccgc gaggcggtag gcagctcggc acttttcgcc gcccgttttc gcgaatgcgc
    38521 cgcgcgggca ttattaatgc cggggcgcac tccgggccat cgcaccccgc tttggcaaca
    38581 acggctgcgc gccagtcagt tgctggaaat cgctcaggga tatccggatt ttccggtcat
    38641 tctcgaaacc ctacgcgaat gtctgcaaga tgtttatgat cttcccgcac tggaacgttt
    38701 gatgcgtcgc ctgaacggtg gcgaaattca aatatccgat gtaacgacca ctacgccctc
    38761 gcctttcgcc acaagtttat tgttcggcta tgtcgcggaa tttatgtacc agagcgacgc
    38821 cccgctggca gagcgccggg catccgtact gtcgctggac agcgagttac tgcgcaatct
    38881 actcggacag gtcgatccgg gggaattact cgacccgcag gtcattcgcc aggtggaaga
    38941 agagttgcaa cgactggctc ctggcagaag agcgaaaggt gaagaaggat tgttcgacct
    39001 gctgtgcgaa ctggggccaa tgaccgttga agacctggcg caacggcata caggcagcag
    39061 tgaagaggtt gcgtcgtatc tggaaaatct tcttgcagta aaacgaatct tcccagcgat
    39121 gattagcgga caggagcgtc ttgcctgtat ggatgatgcc gccaggctgc gtgatgccct
    39181 cggcgtacga ctaccagagt cattgccaga gatttattta catagagtca gttacccgct
    39241 tcgcgacctc tttctgcgct atctccgggc tcatgctctg gtcacggctg aacaactggc
    39301 tcatgagttt agtctcggta ttgccattgt cgaagagcag cttcagcaac tgcgtgaaca
    39361 gggtctggtg atgaatctgc aacaagacat ctgggtgagc gatgaagtat ttcgtcgtct
    39421 gcgtttgcgc tcgctgcaag ccgccagaga agcgacgcgt cccgttgcag ccacgaccta
    39481 tgcgcgattg ctgctggaac gtcagggcgt attacccgcc accgatggta gcccggcgct
    39541 ctttgcctca acatcgccag gcgtttatga gggcgtagat ggcgtgatgc gggtgatcga
    39601 acagcttgcc ggagtcggtt tacccgcctc actctgggaa agccagatcc tgcctgcccg
    39661 cgtacgcgac tattcgtcag aaatgctcga tgaattactg gcaaccggtg cggttatctg
    39721 gtcggggcaa aaaaaactgg gtgaagatga cggcctggtg gcactgcatc tacaggaata
    39781 tgctgcagaa tcgttcactc ccgccgaagc ggatcaggcg aatcgttcgg cgctgcaaca
    39841 agcgatagtc gctgttctgg ctgacggagg agcctggttt gcacaacaaa tcagccaacg
    39901 gatacgcgac aaaatcggcg aatcggttga tctctctgcc ctgcaagagg cgttatgggc
    39961 gctggtctgg caaggcgtca tcaccagcga catttgggca ccgttacgcg ccctcacccg
    40021 cagcagttcc aacgcacgca cctcaactcg ccgcagtcac cgggctcgtc gtggacgtcc
    40081 tgtctatgcg caacccgtct cgccgctggt atcttacaac acaccaaatc tggctggacg
    40141 ctggtcgtta ttgcaggtgg agccactaaa cgataccgaa aggatgctgg cgctggcgga
    40201 aaatatgctc gaccgctacg gcatcatcag tcgtcaggcg gtgatagccg aaaatatccc
    40261 tggcgggttt ccatcgatgc aaacgctttg tcgaagtatg gaagactccg ggcgaattat
    40321 gcgaggtcgt tttgtagaag gtctgggtgg cgcgcaattc gctgaacgtc tgactattga
    40381 ccgattgcgc gatctggcga cacaagccac gcaaacgcgc cactatacac cagtggcgct
    40441 ctctgccaac gatccggcta atgtgtgggg aaatcttctg ccctggcctg cacatccggc
    40501 aacgctggtt ccaacgcgtc gggcgggtgc gctggtggtc gtttctggcg gcaaattgtt
    40561 actctatctg gcgcaaggtg gcaaaaaaat gctggtctgg caggaaaaag aggaattact
    40621 cgccccagag gttttccacg cgctgactac cgcactgcgt cgcgaaccac ggctgcgctt
    40681 tacgctaaca gaagtgaatg atctaccggt ccggcaaacg ccgatgttta cgctgctgcg
    40741 tgaggcggga ttttcaagtt cgccacaagg gctggattgg ggatagagaa aggactgacg
    40801 gatgcccgtt cgcatccgtc agtattgcag gacggattat tccgcgtccg gctcttcaga
    40861 cttgtattta gcggcagttt ctttgatcag ctgctgcagt tcgccacgct gatacatttc
    40921 gatcacgata tcacaaccgc cgaccagctc gccgtcaacc cacagttgcg ggaaggtcgg
    40981 ccagttagca tatttcggca gttccgcacg aatgtccgga ttctgcagaa tatcaacata
    41041 ggcaaaacgt tcgccacatg cggcaagcgc ctggactgcc tgggcagaga aaccgcagct
    41101 cggcagtttc ggtgaacctt tcatgtacag caggatcggg ttttcagcaa tctggcgttg
    41161 gattttttcg atagtggtgc tcattgtctt gcttcctcaa acttctttac ggcagtaatc
    41221 tgacattgta gcgggtcagt gcggcatcgg aaaataacat tttcatcacg cttttgctat
    41281 tttatccctt tgctctattt gttgcatttc aaatattcgt ttcttctctg atgccggcaa
    41341 actggcgtat ttataaccat ttttagcttt cactgctgca tttttttgcg ctcgccaacg
    41401 aaacgtattt tttaacaata aaagctatta actttctctt cttctatgca ttagaatcat
    41461 caagttttgt aaatcagacg caggcatgat agacctgcct taacagaggg acgctcagtg
    41521 gcgcggataa accgtatttc gatcacgctc tgtgctttac tttttaccac cctgccttta
    41581 acgcctatgg cccatgcttc aaagcaagcc agggagagtt ctgctaccac tcatatcacc
    41641 aaaaaagcag ataaaaagaa aagcacggca accacccaaa agacccagaa aacagcgaaa
    41701 aaagccgcca gtaaaagtac gaccaaaagc aaaaccgctt cttccgttaa aaaatcttcc
    41761 attaccgctt ctaaaaacgc caaaactcgc agcaaacacg ccgtcaataa aacggcctca
    41821 gccagcttta ccgaaaagtg taccaaacgt aagggctata aatcgcattg tgtgaaagtc
    41881 aaaaatgccg cgtcaggaac tcttgccgac gcgcacaaag cgaaggtgca aaaagctacg
    41941 aaagtggcaa tgaataaact gatgcagcaa attggtaagc catatcgttg gggtggcagc
    42001 tcaccgcgta ccggttttga ttgcagcggc ctggtttatt acgcttataa agatttggtg
    42061 aaaattcgta ttccgcgtac ggcgaatgaa atgtatcacc tgcgtgatgc agcgccaatc
    42121 gaacgtagtg aacagaaaaa cggcgacctg gtctttttcc gtactcaggg acgcggcaca
    42181 gccgatcatg tcggcgtgta tgtcggcaac ggcaaattta ttcagtcacc gcgcacaggt
    42241 caggaaattc aaatcacttc tctcagtgaa gactactggc agcgccacta tgttggcgct
    42301 cgtcgggtaa tgaccccaaa aacacttcgc taaaacttta ccctgttgtt acggcaacag
    42361 ggtaagttca tcttttgtct caccttttaa tttgctaccc tatccatacg cacaataagg
    42421 ctattgtacg tatgcaaatt aataataaag gagagtagca atgtcattcg aattacctgc
    42481 actaccatat gctaaagatg ctctggcacc gcacatttct gcggaaacta tcgagtatca
    42541 ctacggcaag catcatcaga cttatgtcac taacctgaac aacctgatta aaggtaccgc
    42601 gtttgaaggt aaatcactgg aagagattat tcgcagctct gaaggtggcg tattcaacaa
    42661 cgcagctcag gtctggaacc atactttcta ctggaactgc ctggcaccga acgccggtgg
    42721 cgaaccgact ggaaaagtcg ctgaagctat cgccgcatct tttggcagct ttgccgattt
    42781 caaagcgcag tttactgatg cagcgatcaa aaactttggt tctggctgga cctggctggt
    42841 gaaaaacagc gatggcaaac tggctatcgt ttcaacctct aacgcgggta ctccgctgac
    42901 caccgatgcg actccgctgc tgaccgttga tgtctgggaa cacgcttatt acatcgacta
    42961 tcgcaatgca cgtcctggct atctggagca cttctgggcg ctggtgaact gggaattcgt
    43021 agcgaaaaat ctcgctgcat aataactgat ggcaaatgca gcattgcctg atgcgcttcg
    43081 cttaccatgc caacgcggat gaggcgttaa cagcgcattc ggcaagataa atcgcacgtt
    43141 gtcagcaact gtaacgcaga aggttatcct tctgcgtttt tgtttaatta gctgttagca
    43201 acgcaaactg tttcaggttg ttttctggct gacataaaca ccagcaataa tgccagtccc
    43261 gcgacaatcg ctcccatcac cggcacaaag ctgtatccca gcccagcgga aattaccgca
    43321 ccaccagcag ctgctcccag cgcatttcca agattaaagg caccaatatt gactgatgaa
    43381 gacagacctg gcgcttcact ggcgacacgc atcacgcgca tctgtaacgg cggtacgacc
    43441 gcaaaggttg ctgcgcccca caccaccatg ctaatagctg cgccgaactc attgcgggcc
    43501 aggaacggga ttgccagcat aatcaccatc aacaacaaca aaaagccttt caacgtgccg
    43561 ttaactgaac gatctgccag tttgccgccg agatagttac cgatagagaa tccgacacca
    43621 atcagcacca gcattgccgt gacgaacacc ggtgttgcgt gggtaatact ttgcagtacc
    43681 ggagagatat aggtgtagag agtaaacatt gcaccagctc ccagtaccgt cgtcagcaat
    43741 gcagacagca cctgcggacg cattaatacc gccagctctt ttttcacttc aggtcgtgcc
    43801 cctgcaccac ctttaggtaa tgagaagaac agacttacca ttgaaatcac tcccagcccc
    43861 gccgttgcca aaaatgacat ccgccagccg atggtttcac ccaaccaggt cgccgccggc
    43921 acgccaccga tatttgccag ggttaacccc ataaacatag tggcaactgc gctggcctgt
    43981 ttatgttttg gcaccacgct tgcggccacg actgaaccca aacccaaaaa tgctccgtga
    44041 ttcaggctgg tcgaaatgcg tgaaagcatc agggtcatat aatccggcgc gatggcggaa
    44101 agtacgttgc cgagcgtgaa aattgccatc aggaaaatca acgcactgcg gcgggcacga
    44161 tgagaaagta gaagcgtcat cagcggcgcg ccaaccatta cgccaactgc ataggcactg
    44221 attaacattc cggcagcggg aatcgagaca tccacaccgc gcgcaatgac gggcaacaag
    44281 cccattggcg agaactccgt tgtcccgata ccaaacgcgc caatcgccag cgccagcgcc
    44341 agcaacggat agttaatttt catgccttat ctccacctct tcgcgtcatt acgcgatatt
    44401 cattaaagtg gcgaaagcat gacagcaatc acaaaaaaat gaaaataaca aaaagagaaa
    44461 acacttttgc cattttgcta acaaacagga aggagatgcg agggagaacg cgctccctcg
    44521 agaggaaatc agtgcagcgc ggcagtcaaa cccacggcca cgatcaaacc gaggacgata
    44581 atcgttgtta ccagtgaaaa tttaaggtcg gtgctcatca agttttctcc ttttttatta
    44641 ccacacaaat agtgatatta cgcattttta cacactgtga tgaaaaaatc tcccgtcatt
    44701 tataatgata agtgttttta ccacttcccc ttttcgtcaa gatcggccaa aattccacgc
    44761 ttacactatt tgcgtactgg ccattgaccc cttcctgacg ctccgtgtcg tttttccggc
    44821 gtaccgcaac acttttgttg tgcgtaaggt gtgtaaaggc aaacgtttac cttgcgattt
    44881 tgcaggagct gaagttaggg tctggagtga aatggaatgg caacaataaa agatgtagcg
    44941 aaacgagcaa acgtttccac tacaactgtg tcacacgtaa tcaacaaaac acgtttcgtc
    45001 gctgaagaaa cgcgcaacgc cgtgtgggca gcgattaaag aattacacta ctcccctagc
    45061 gcggtggcgc gtagcctgaa ggttaaccac accaagtcta tcggtttgct ggcgaccagc
    45121 agcgaagcgg cctattttgc cgagatcatt gaagcggttg aaaaaaattg cttccagaaa
    45181 ggttacaccc tgattctggg taatgcgtgg aacaatcttg agaaacagcg ggcttatctg
    45241 tcgatgatgg cgcaaaaacg cgtcgatggt ctgctggtga tgtgttctga gtacccagag
    45301 ccgttgctgg cgatgctgga agagtatcgc catatcccaa tggtggtgat ggactggggt
    45361 gaagcaaaag ctgacttcac cgatgcggtc attgataacg cgttcgaagg cggctacatg
    45421 gccgggcgtt atctgattga acgcggtcac cgcgaaatcg gcgtcatccc cggcccgctg
    45481 gaacgtaaca ccggcgcagg ccgccttgcc ggttttatga aggcgatgga agaagcgatg
    45541 atcaaggtgc cggaaagctg gattgttcag ggtgactttg aacccgaatc tggttatcgc
    45601 gccatgcagc aaatactgtc gcagccgcat cgccctactg ccgtcttctg tggtggcgat
    45661 atcatggcaa tgggcgcact ttgtgctgct gatgaaatgg gtctgcgcgt cccgcaggat
    45721 gtttcgctga tcggttatga taacgtgcgc aacgcgcgct attttacgcc ggcgctaacc
    45781 acgatccacc agccaaaaga ttcgctgggt gaaacagcgt tcaacatgct gttggatcgt
    45841 atcgtcaaca aacgtgaaga accgcagtcc attgaagtgc atccgcgctt gattgaacgc
    45901 cgctccgtgg ctgacggccc gttccgcgac tatcgtcgtt aatcacccgt tgcgggagtc
    45961 tcttccggct cccgcagcca ctccttattc agcgtctcac tatcgccgag atactcaagc
    46021 agccaggtta acgcaggcga catatcattt tgctgccatg tcagacaaca tgccgaatcc
    46081 ggaaaggggt tttccagttc taatgccacc cacttcccct cattaagcca cggtttggcg
    46141 aaatgtgttg gcaccatccc tatgcataat cctgccgaga tacaggttgc cgatgattcc
    46201 cagtcaggca cgacaactct tttttggtta tccagcaacc aggtaatacg tttaggtagc
    46261 gttcgcgagg tgtcttcgcg caccaacgac ggccagttgc gcaacgtatc atcgctgaac
    46321 gggccatcca tcaacgccag cgggtggtgg ctggcaacaa cgcaactcca gcttagcatc
    46381 cccatatccc ggaaggcata acgaccgcct accggaatcg cgcgtgttgc gccaatcgcc
    46441 agttccacgc gcccatcgga aagcgcatcc cagacaccgt taaacacttc ctgaaagacc
    46501 agaagttcga cgtcgtcaaa atggcgataa aaatcaacga tcatctgccg tgtacgttct
    46561 ggcctgacaa tattatccac tgcgatagct aactgaccgc gccagccgtt cgctatctgc
    46621 tgacattgct ggcgggtgat ctgcattttt ttgacaacag agcgcccttc tttgagaaac
    46681 cacgctccgg cagcggtcag ttccacatca cggtgccgtc tttcaaagag cggcaccgcc
    46741 agccactctt ccagctgacg cacggtatag ctgaccgcag aaggaacgcg atgcaactcc
    46801 tgtgccgcag cgctaaaact accattacgc gctaccgcat caacaacttc gagagaatat
    46861 tctgaccaca tagtctgcct gcaaaatttt tgaaaccagt catcaaatat taccgtttca
    46921 caacactaat ttcactccct acactttgcg gcggtgttta attgagagat ttagagaata
    46981 tacatgcaac ctgggaaaag atttttagtc tggctggcgg gtttgagcgt actcggtttt
    47041 ctggcaaccg atatgtatct gcctgctttc gccgccatac aggccgacct gcaaacgcct
    47101 gcgtctgctg tcagtgccag ccttagtctg ttccttgccg gttttgccgc agcccagctt
    47161 ctgtgggggc cgctctccga ccgttatggt cgtaaaccgg tgttattaat cggcctgaca
    47221 atttttgcgt taggtagtct ggggatgctg tgggtagaaa acgccgctac gctgctggta
    47281 ttgcgttttg tacaggctgt gggtgtctgc gccgcggcgg ttatctggca agcgttagtg
    47341 acggattatt ttccttcaca gaaagttaac cgaatttttg cgaccatcat gccgctggtg
    47401 ggtctatctc cggccctggc tcctctgtta ggaagctggc tgctggtcca tttttcctgg
    47461 caggcgattt tcgccaccct gtttgccatt accgtggtgc tgattctgcc tattttctgg
    47521 ctcaaaccca cgacgaaggc ccgtaacaat agtcaggatg gtctgacctt taccgacctg
    47581 ctacgttcta aaacctatcg cggcaacgtg ctgatatacg cggcctgttc agccagtttt
    47641 tttgcatggc tgaccggttc accgttcatc cttagtgaaa tgggctacag cccggcagtt
    47701 attggtttaa gttatgtccc gcaaactatc gcgtttctga ttggtggtta tggctgtcgc
    47761 gccgcgctgc agaaatggca aggcaagcag ttattaccgt ggttgctggt gctgtttgca
    47821 gtcagcgtca ttgcgacctg ggctgcaggc ttcattagcc atgtgtcgct ggtcgaaatc
    47881 ctgatcccat tctgtgtgat ggcgattgcc aatggcgcga tctaccctat tgttgtcgcc
    47941 caggcgctgc gtcccttccc acacgcaact ggtcgcgccg cagcgttgca gaacactcta
    48001 caactgggtc tgtgcttcct cgcaagtctg gtagtttcct ggctgatcag tatcagcacg
    48061 ccattgctca ccaccaccag cgtgatgtta tcaacagtag tgctggtcgc gctgggttac
    48121 atgatgcaac gttgtgaaga agttggctgc cagaatcatg gcaatgccga agtcgctcat
    48181 agcgaatcac actgatctat atcgatatac ttatacttag gctgctaaca taatttttgt
    48241 tgtatgattg aaattagcgg cctatactaa tttcgagttg ttaaagctac gataaatatt
    48301 atgtttttac ggggacagga tcgttcccga ctcactatgg atggtcattt cggcaagggt
    48361 tcctcctttc cctctgttct acgtcggatt atagactcgc ggttttttct gcgagatttc
    48421 tcacaaagcc caaaaagcgt ctacgctgtt ttaaggttct gatcaccgac tagtgatgga
    48481 gaaactatga gttcatcgtg tatagaagaa gtcagtgtac cggatgacaa ctggtaccgt
    48541 atcgccaacg aattacttag ccgtgccggt atagccatta acggttctgc cccggcggat
    48601 attcgtgtga aaaaccccga tttttttaaa cgcgttctgc aagaaggctc tttggggtta
    48661 ggcgaaagtt atatggatgg ctggtgggaa tgtgaccgac tggatatgtt ttttagcaaa
    48721 gtcttacgcg caggtctcga gaaccaactc ccccatcatt tcaaagacac gctacgtatt
    48781 gccggcgctc gtctcttcaa tctgcagagt aaaaaacgtg cctggatagt cggcaaggag
    48841 cattacgatt tgggtaatga cttgttcagc cgcatgcttg atcccttcat gcaatattca
    48901 tgcgcttact ggaaagatgc cgataatctg gaatctgccc agcaggcgaa gctcaaaatg
    48961 atttgtgaaa aattgcagtt aaaaccaggg atgcgcgtac tggatattgg ctgcggctgg
    49021 ggcggactgg cgcactacat ggcatctaac tatgacgtaa gcgtggtcgg cgtcaccatt
    49081 tctgccgaac agcaaaaaat ggctcaggaa cgctgtgaag gcctggatgt caccattttg
    49141 ctgcaagatt atcgtgacct gaacgaccag tttgatcgta ttgtttctgt ggggatgttc
    49201 gagcacgtcg gaccgaaaaa ttacgatacc tattttgcgg tggtggatcg taatttgaaa
    49261 ccggaaggta tattcctgct ccatactatc ggttcgaaaa aaaccgatct gaatgttgat
    49321 ccctggatta ataaatatat ttttccgaac ggttgcctgc cctctgtacg ccagattgct
    49381 caatccagcg aaccccactt tgtgatggaa gactggcata acttcggtgc tgattacgat
    49441 actacgttga tggcgtggta tgaacgattc ctcgccgcat ggccagaaat tgcggataac
    49501 tacagtgaac gctttaaacg aatgtttacc tattatctga atgcctgtgc aggtgctttc
    49561 cgcgcccgtg atattcagct ctggcaggtc gtgttctcac gcggtgttga aaacggcctt
    49621 cgagtggctc gctaaaggct attctatcgc cccctctccg ggggcgattt cagatcaggc
    49681 ttctgtacct ggttgattca tggcattttc tcgtgccgcc agcacacgtt ctaccgtatc
    49741 taccactgcc tgagtttgtg gatcgatttc aatgttgacg cgtgcgccaa gttttttctt
    49801 cccaagagtc gtgcgttcca gtgtttccgg aattaaatgg acgcaaaaac gcgttggcgt
    49861 gacttcgccg acggtcaggc taataccgtc gatgccaata aatcctttgt acagaatata
    49921 tttcatcaac tgactatcct ggactttaaa ccagatctgg cgattatttt ctgaggttaa
    49981 tattttcgcc acttcagcag tggtcataat atgacctgac attaagtgtc cgccaatttc
    50041 atcactgaat ttcgccgcac gctcaacgtt tacccaatcc cccactttta aatcgccaag
    50101 attggtaatg cgtaacgttt ctttcatcag gtcaaaactg acatggttgc cgttaatttc
    50161 cgtcacggtc aggcagcaac cgttatgcgc cacggaagca cctgtttcca ggccgtccag
    50221 catgtggtcg ggtaactcca ccacatgcgt acgaaaattt ggtttctcgt caatcgacac
    50281 cagttttgcg gtgccctgta caatccccgt aaacatactt acaactcctg aaatcagtta
    50341 agacattctg ttcagcacaa tagcaggtgg caaacgccct taccagtgaa ggggtaagaa
    50401 tgactatttt ttcactggag aattaataaa tcctcgctac aatagactga atttcccctg
    50461 cttcttcttt ttgctgccca ttcaggcggc tttttagtct ctcatataac tacaaataaa
    50521 aggtgttcac gtgcagaagt atatcagtga agcgcgtctg ttattagcat tagcaatccc
    50581 ggtgattctc gcgcaaatcg cccaaactgc aatgggtttt gtcgataccg tgatggcggg
    50641 cggctatagt gccaccgaca tggcggcggt cgctatcggt acttctatct ggcttccggc
    50701 gatcctcttt ggtcacggac tgctgctggc attaacgccg gttatcgcgc aattaaatgg
    50761 ttccggtcga cgtgagcgca ttgcgcatca ggtgcgacaa ggtttctggc tggcaggttt
    50821 tgtttccgtt ctcattatgc tggtgctgtg gaatgcaggt tacattatcc gctccatgga
    50881 aaacatcgat ccggctctgg cggacaaagc cgtgggttat ctgcgtgcgt tgttgtgggg
    50941 cgcgccgggc tatctgttct tccaggttgc ccgtaaccaa tgtgaaggtc tggcaaaaac
    51001 caagccgggt atggtaatgg gctttatcgg cctgctggtg aacattccgg tgaactatat
    51061 ctttatttat ggtcatttcg gtatgcctga gctcggtggc gttggttgtg gcgtggctac
    51121 tgcggcggtg tattgggtca tgttccttgc catggtttct tacattaaac gcgcccgctc
    51181 catgcgcgat attcgtaacg aaaaaggcac cgcgaaaccc gatcctgcgg ttatgaaacg
    51241 actgattcaa ctcggtttgc cgattgcgct ggcactattc tttgaagtga cactgtttgc
    51301 cgtcgtggct ctgttagtgt ctccgctcgg tattgttgat gtcgcaggac accagattgc
    51361 cctgaacttt agttcactga tgtttgtgct tccaatgtcg ctggcggcag cggtaactat
    51421 ccgcgtaggt tatcgtctgg gtcagggctc aacgctggat gcgcaaaccg ctgcgcggac
    51481 cgggcttatg gtgggtgtct gtatggcaac cctgacggcc atattcacgg tttcactgcg
    51541 ggagcaaatc gccctgttgt acaacgacaa tcccgaggtt gtaacgctgg ctgcgcattt
    51601 gatgttgctg gcggcggtat atcagatttc tgactcaatc caggtgattg gcagtgggat
    51661 tttgcgtggt tataaagata cgcgttccat tttctatatc acctttacgg cttactgggt
    51721 gctgggcttg ccaagcggct atattctggc actgaccgat ctggtcgttg aacctatggg
    51781 gccagcaggc ttctggatag gctttattat tggcctgacg tcggcagcca ttatgatgat
    51841 gctgcgtatg cggttcctgc aacgtctgcc gtcagttatc attctgcaac gagcatcccg
    51901 ctaataaaga caaggcgcaa ccttcacggg ttgcgcctgt gtttttacgc aggctggagc
    51961 g
//
LOCUS       DAZKHC020000023        51940 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZKHC020000023 DAZKHC020000000
VERSION     DAZKHC020000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 51940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.023"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            156..584
                     /gene="uspC"
                     /locus_tag="ACHOSM_RS08020"
                     /old_locus_tag="pgaptmp_001604"
     CDS             156..584
                     /gene="uspC"
                     /locus_tag="ACHOSM_RS08020"
                     /old_locus_tag="pgaptmp_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HGU4558154.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(591..2015)
                     /gene="otsA"
                     /locus_tag="ACHOSM_RS08025"
                     /old_locus_tag="pgaptmp_001605"
     CDS             complement(591..2015)
                     /gene="otsA"
                     /locus_tag="ACHOSM_RS08025"
                     /old_locus_tag="pgaptmp_001605"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HGU4558155.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQAPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1990..2790)
                     /gene="otsB"
                     /locus_tag="ACHOSM_RS08030"
                     /old_locus_tag="pgaptmp_001606"
     CDS             complement(1990..2790)
                     /gene="otsB"
                     /locus_tag="ACHOSM_RS08030"
                     /old_locus_tag="pgaptmp_001606"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HGU4558156.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2957..3943)
                     /gene="araH"
                     /locus_tag="ACHOSM_RS08035"
                     /old_locus_tag="pgaptmp_001607"
     CDS             complement(2957..3943)
                     /gene="araH"
                     /locus_tag="ACHOSM_RS08035"
                     /old_locus_tag="pgaptmp_001607"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HGU4558157.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(3958..5472)
                     /gene="araG"
                     /locus_tag="ACHOSM_RS08040"
                     /old_locus_tag="pgaptmp_001608"
     CDS             complement(3958..5472)
                     /gene="araG"
                     /locus_tag="ACHOSM_RS08040"
                     /old_locus_tag="pgaptmp_001608"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HGU4558158.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIFYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5542..6531)
                     /gene="araF"
                     /locus_tag="ACHOSM_RS08045"
                     /old_locus_tag="pgaptmp_001609"
     CDS             complement(5542..6531)
                     /gene="araF"
                     /locus_tag="ACHOSM_RS08045"
                     /old_locus_tag="pgaptmp_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HGU4558159.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGDK"
     gene            7328..7831
                     /gene="ftnB"
                     /locus_tag="ACHOSM_RS08050"
                     /old_locus_tag="pgaptmp_001610"
     CDS             7328..7831
                     /gene="ftnB"
                     /locus_tag="ACHOSM_RS08050"
                     /old_locus_tag="pgaptmp_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HGU4558160.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7910..8161)
                     /gene="yecJ"
                     /locus_tag="ACHOSM_RS08055"
                     /old_locus_tag="pgaptmp_001611"
     CDS             complement(7910..8161)
                     /gene="yecJ"
                     /locus_tag="ACHOSM_RS08055"
                     /old_locus_tag="pgaptmp_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HGU4558161.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8276..8362)
                     /gene="azuC"
                     /locus_tag="ACHOSM_RS08060"
                     /old_locus_tag="pgaptmp_001612"
     CDS             complement(8276..8362)
                     /gene="azuC"
                     /locus_tag="ACHOSM_RS08060"
                     /old_locus_tag="pgaptmp_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HGU4558162.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8625..8948
                     /gene="yecR"
                     /locus_tag="ACHOSM_RS08065"
                     /old_locus_tag="pgaptmp_001613"
     CDS             8625..8948
                     /gene="yecR"
                     /locus_tag="ACHOSM_RS08065"
                     /old_locus_tag="pgaptmp_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HGU4558163.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDHAFLQ
                     HAHTDPYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFTGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9119..9616
                     /gene="ftnA"
                     /locus_tag="ACHOSM_RS08070"
                     /old_locus_tag="pgaptmp_001614"
     CDS             9119..9616
                     /gene="ftnA"
                     /locus_tag="ACHOSM_RS08070"
                     /old_locus_tag="pgaptmp_001614"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HGU4558164.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(9653..9892)
                     /gene="yecH"
                     /locus_tag="ACHOSM_RS08075"
                     /old_locus_tag="pgaptmp_001615"
     CDS             complement(9653..9892)
                     /gene="yecH"
                     /locus_tag="ACHOSM_RS08075"
                     /old_locus_tag="pgaptmp_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HGU4558165.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10084..11295
                     /gene="tyrP"
                     /locus_tag="ACHOSM_RS08080"
                     /old_locus_tag="pgaptmp_001616"
     CDS             10084..11295
                     /gene="tyrP"
                     /locus_tag="ACHOSM_RS08080"
                     /old_locus_tag="pgaptmp_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HGU4558166.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11357..12022)
                     /gene="yecA"
                     /locus_tag="ACHOSM_RS08085"
                     /old_locus_tag="pgaptmp_001617"
     CDS             complement(11357..12022)
                     /gene="yecA"
                     /locus_tag="ACHOSM_RS08085"
                     /old_locus_tag="pgaptmp_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HGU4558167.1"
                     /translation="MKTGPLNESELEWLDDNLTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12218..12304)
                     /locus_tag="ACHOSM_RS08090"
                     /old_locus_tag="pgaptmp_001618"
     tRNA            complement(12218..12304)
                     /locus_tag="ACHOSM_RS08090"
                     /old_locus_tag="pgaptmp_001618"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12268..12270),aa:Leu,seq:taa)
     gene            complement(12317..12390)
                     /locus_tag="ACHOSM_RS08095"
                     /old_locus_tag="pgaptmp_001619"
     tRNA            complement(12317..12390)
                     /locus_tag="ACHOSM_RS08095"
                     /old_locus_tag="pgaptmp_001619"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12356..12358),aa:Cys,seq:gca)
     gene            complement(12444..12519)
                     /locus_tag="ACHOSM_RS08100"
                     /old_locus_tag="pgaptmp_001620"
     tRNA            complement(12444..12519)
                     /locus_tag="ACHOSM_RS08100"
                     /old_locus_tag="pgaptmp_001620"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12484..12486),aa:Gly,seq:gcc)
     gene            complement(12671..13219)
                     /gene="pgsA"
                     /locus_tag="ACHOSM_RS08105"
                     /old_locus_tag="pgaptmp_001621"
     CDS             complement(12671..13219)
                     /gene="pgsA"
                     /locus_tag="ACHOSM_RS08105"
                     /old_locus_tag="pgaptmp_001621"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGU4558168.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13276..15108)
                     /gene="uvrC"
                     /locus_tag="ACHOSM_RS08110"
                     /old_locus_tag="pgaptmp_001622"
     CDS             complement(13276..15108)
                     /gene="uvrC"
                     /locus_tag="ACHOSM_RS08110"
                     /old_locus_tag="pgaptmp_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGU4558169.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15105..15761)
                     /gene="uvrY"
                     /locus_tag="ACHOSM_RS08115"
                     /old_locus_tag="pgaptmp_001623"
     CDS             complement(15105..15761)
                     /gene="uvrY"
                     /locus_tag="ACHOSM_RS08115"
                     /old_locus_tag="pgaptmp_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HGU4558170.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16057..16233
                     /gene="yecU"
                     /locus_tag="ACHOSM_RS08120"
                     /old_locus_tag="pgaptmp_001624"
     CDS             16057..16233
                     /gene="yecU"
                     /locus_tag="ACHOSM_RS08120"
                     /old_locus_tag="pgaptmp_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HGU4558171.1"
                     /translation="MIKIFIGHYINAFNSTVDDTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            16220..16444
                     /gene="yecF"
                     /locus_tag="ACHOSM_RS08125"
                     /old_locus_tag="pgaptmp_001625"
     CDS             16220..16444
                     /gene="yecF"
                     /locus_tag="ACHOSM_RS08125"
                     /old_locus_tag="pgaptmp_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HGU4558172.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(16512..17234)
                     /gene="sdiA"
                     /locus_tag="ACHOSM_RS08130"
                     /old_locus_tag="pgaptmp_001626"
     CDS             complement(16512..17234)
                     /gene="sdiA"
                     /locus_tag="ACHOSM_RS08130"
                     /old_locus_tag="pgaptmp_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HGU4558173.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQLLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRGLGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(17464..18216)
                     /gene="tcyN"
                     /locus_tag="ACHOSM_RS08135"
                     /old_locus_tag="pgaptmp_001627"
     CDS             complement(17464..18216)
                     /gene="tcyN"
                     /locus_tag="ACHOSM_RS08135"
                     /old_locus_tag="pgaptmp_001627"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HGU4558174.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(18213..18881)
                     /gene="tcyL"
                     /locus_tag="ACHOSM_RS08140"
                     /old_locus_tag="pgaptmp_001628"
     CDS             complement(18213..18881)
                     /gene="tcyL"
                     /locus_tag="ACHOSM_RS08140"
                     /old_locus_tag="pgaptmp_001628"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HGU4558175.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(18896..19882)
                     /gene="dcyD"
                     /locus_tag="ACHOSM_RS08145"
                     /old_locus_tag="pgaptmp_001629"
     CDS             complement(18896..19882)
                     /gene="dcyD"
                     /locus_tag="ACHOSM_RS08145"
                     /old_locus_tag="pgaptmp_001629"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HGU4558176.1"
                     /translation="MQLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALRDGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(19987..20787)
                     /gene="tcyJ"
                     /locus_tag="ACHOSM_RS08150"
                     /old_locus_tag="pgaptmp_001630"
     CDS             complement(19987..20787)
                     /gene="tcyJ"
                     /locus_tag="ACHOSM_RS08150"
                     /old_locus_tag="pgaptmp_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4558177.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(20875..21426)
                     /gene="fliZ"
                     /locus_tag="ACHOSM_RS08155"
                     /old_locus_tag="pgaptmp_001631"
     CDS             complement(20875..21426)
                     /gene="fliZ"
                     /locus_tag="ACHOSM_RS08155"
                     /old_locus_tag="pgaptmp_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HGU4558178.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCIGLVQKSSSLPASDIY"
     gene            complement(21472..22191)
                     /gene="fliA"
                     /locus_tag="ACHOSM_RS08160"
                     /old_locus_tag="pgaptmp_001632"
     CDS             complement(21472..22191)
                     /gene="fliA"
                     /locus_tag="ACHOSM_RS08160"
                     /old_locus_tag="pgaptmp_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HGU4558179.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPTSVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRGSQLHSQAIKRLRTKLGKL"
     gene            complement(22355..23686)
                     /locus_tag="ACHOSM_RS08165"
                     /old_locus_tag="pgaptmp_001633"
     CDS             complement(22355..23686)
                     /locus_tag="ACHOSM_RS08165"
                     /old_locus_tag="pgaptmp_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HGU4558180.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSTSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISLAQTTEGALSEINNNLQRVRELTVQATTG
                     TNSDSDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGQTISIDL
                     KKIDSSTLGLNGFSVSKNSLNVSNAITSIPQDAGDKPVDVNFGTTTESAAIAAKLGVS
                     DTSSLSLHNILDKDGKATADYVVQSGKDFYAASVDAASGKVTLNTIDVTYDDYANGVD
                     DAKQTDQLIKVSADKDGAAQGFVTLQGKNYSAGDAAVILKNGATGLALTDLNLSDVTD
                     TNGKVTTTATEQFEGASTEDPLALLDKAIASVDKFRSSLGAVQNRLDSAITNLNNTTT
                     NLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLSKANQVPQQVLSLLQG"
     gene            23932..25347
                     /gene="fliD"
                     /locus_tag="ACHOSM_RS08170"
                     /old_locus_tag="pgaptmp_001634"
     CDS             23932..25347
                     /gene="fliD"
                     /locus_tag="ACHOSM_RS08170"
                     /old_locus_tag="pgaptmp_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGU4558181.1"
                     /translation="MASISTLGVGSGLDLSSILDSLEAAEKSTLTPISKQQSSYTAKL
                     SAYGTLKSALESFQTANTALNKADLFTATSTTSSSSAFSATTTGSAIAGKYTISVSQL
                     AQAQTLTTKISQKDSKAAIATCDSVLTIQQGGGKDPVTIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSITANDTGNDNAMKLSVSGDSALESFMGYNGTSGDSSNGM
                     IESVTAQNAKLTVNNVEIENSSNTISDALEDITLNLNDVTTGNQTLTISKDTSKAENV
                     VKAWVDAYNTLQDTFSSLTKYTAVDAGAESQDSSNGALLGDSTLRTIQTQLKTLLANT
                     HSSSNYKTLAQIGITSDASTGKLEIATDKLQTALKKDAAGIGEMFIGDGKSTGVTTGI
                     SSNLTSWLSSTGIIQAAKDGVSKTLNNLTDQYNAASERIDTLMTRYKAQFTQLDVLMN
                     SLNSTSSYLTQQFDTSNSNSK"
     gene            25363..25773
                     /gene="fliS"
                     /locus_tag="ACHOSM_RS08175"
                     /old_locus_tag="pgaptmp_001635"
     CDS             25363..25773
                     /gene="fliS"
                     /locus_tag="ACHOSM_RS08175"
                     /old_locus_tag="pgaptmp_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGU4558182.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLTPSLIQDPV"
     gene            25773..26138
                     /gene="fliT"
                     /locus_tag="ACHOSM_RS08180"
                     /old_locus_tag="pgaptmp_001636"
     CDS             25773..26138
                     /gene="fliT"
                     /locus_tag="ACHOSM_RS08180"
                     /old_locus_tag="pgaptmp_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HGU4558183.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26216..27703
                     /gene="amyA"
                     /locus_tag="ACHOSM_RS08185"
                     /old_locus_tag="pgaptmp_001637"
     CDS             26216..27703
                     /gene="amyA"
                     /locus_tag="ACHOSM_RS08185"
                     /old_locus_tag="pgaptmp_001637"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU4558184.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(27737..28150)
                     /gene="yedD"
                     /locus_tag="ACHOSM_RS08190"
                     /old_locus_tag="pgaptmp_001638"
     CDS             complement(27737..28150)
                     /gene="yedD"
                     /locus_tag="ACHOSM_RS08190"
                     /old_locus_tag="pgaptmp_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HGU4558185.1"
                     /translation="MKKLAIASALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            28337..29542
                     /gene="yedE"
                     /locus_tag="ACHOSM_RS08195"
                     /old_locus_tag="pgaptmp_001639"
     CDS             28337..29542
                     /gene="yedE"
                     /locus_tag="ACHOSM_RS08195"
                     /old_locus_tag="pgaptmp_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HGU4558186.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            29539..29772
                     /gene="yedF"
                     /locus_tag="ACHOSM_RS08200"
                     /old_locus_tag="pgaptmp_001640"
     CDS             29539..29772
                     /gene="yedF"
                     /locus_tag="ACHOSM_RS08200"
                     /old_locus_tag="pgaptmp_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HGU4558187.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            29879..30547
                     /gene="yedK"
                     /locus_tag="ACHOSM_RS08205"
                     /old_locus_tag="pgaptmp_001641"
     CDS             29879..30547
                     /gene="yedK"
                     /locus_tag="ACHOSM_RS08205"
                     /old_locus_tag="pgaptmp_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HGU4558188.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQDIGGKEASEIAASGCVPANQFSWHPVSRAVGNIKNQGAEL
                     IQPV"
     gene            complement(30930..31244)
                     /gene="fliE"
                     /locus_tag="ACHOSM_RS08210"
                     /old_locus_tag="pgaptmp_001642"
     CDS             complement(30930..31244)
                     /gene="fliE"
                     /locus_tag="ACHOSM_RS08210"
                     /old_locus_tag="pgaptmp_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HGU4558189.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            31459..33117
                     /gene="fliF"
                     /locus_tag="ACHOSM_RS08215"
                     /old_locus_tag="pgaptmp_001643"
     CDS             31459..33117
                     /gene="fliF"
                     /locus_tag="ACHOSM_RS08215"
                     /old_locus_tag="pgaptmp_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HGU4558190.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEEEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            33110..34105
                     /gene="fliG"
                     /locus_tag="ACHOSM_RS08220"
                     /old_locus_tag="pgaptmp_001644"
     CDS             33110..34105
                     /gene="fliG"
                     /locus_tag="ACHOSM_RS08220"
                     /old_locus_tag="pgaptmp_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HGU4558191.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            34098..34784
                     /gene="fliH"
                     /locus_tag="ACHOSM_RS08225"
                     /old_locus_tag="pgaptmp_001645"
     CDS             34098..34784
                     /gene="fliH"
                     /locus_tag="ACHOSM_RS08225"
                     /old_locus_tag="pgaptmp_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HGU4558192.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQEGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            34784..36157
                     /gene="fliI"
                     /locus_tag="ACHOSM_RS08230"
                     /old_locus_tag="pgaptmp_001646"
     CDS             34784..36157
                     /gene="fliI"
                     /locus_tag="ACHOSM_RS08230"
                     /old_locus_tag="pgaptmp_001646"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HGU4558193.1"
                     /translation="MSTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIEHQNGTETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TDVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            36176..36619
                     /gene="fliJ"
                     /locus_tag="ACHOSM_RS08235"
                     /old_locus_tag="pgaptmp_001647"
     CDS             36176..36619
                     /gene="fliJ"
                     /locus_tag="ACHOSM_RS08235"
                     /old_locus_tag="pgaptmp_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HGU4558194.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            36616..37743
                     /gene="fliK"
                     /locus_tag="ACHOSM_RS08240"
                     /old_locus_tag="pgaptmp_001648"
     CDS             36616..37743
                     /gene="fliK"
                     /locus_tag="ACHOSM_RS08240"
                     /old_locus_tag="pgaptmp_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGU4558195.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KSAPQLLVATDKPTTKGEPLISDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLLVPVSIQGRVTGNSGVDIFA"
     gene            37848..38312
                     /gene="fliL"
                     /locus_tag="ACHOSM_RS08245"
                     /old_locus_tag="pgaptmp_001649"
     CDS             37848..38312
                     /gene="fliL"
                     /locus_tag="ACHOSM_RS08245"
                     /old_locus_tag="pgaptmp_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HGU4558196.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSPPLVAGQPKQDVTDVLYTAFILR"
     gene            38317..39321
                     /gene="fliM"
                     /locus_tag="ACHOSM_RS08250"
                     /old_locus_tag="pgaptmp_001650"
     CDS             38317..39321
                     /gene="fliM"
                     /locus_tag="ACHOSM_RS08250"
                     /old_locus_tag="pgaptmp_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HGU4558197.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            39318..39731
                     /gene="fliN"
                     /locus_tag="ACHOSM_RS08255"
                     /old_locus_tag="pgaptmp_001651"
     CDS             39318..39731
                     /gene="fliN"
                     /locus_tag="ACHOSM_RS08255"
                     /old_locus_tag="pgaptmp_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HGU4558198.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            39734..40099
                     /gene="fliO"
                     /locus_tag="ACHOSM_RS08260"
                     /old_locus_tag="pgaptmp_001652"
     CDS             39734..40099
                     /gene="fliO"
                     /locus_tag="ACHOSM_RS08260"
                     /old_locus_tag="pgaptmp_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HGU4558199.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKLTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            40099..40836
                     /gene="fliP"
                     /locus_tag="ACHOSM_RS08265"
                     /old_locus_tag="pgaptmp_001653"
     CDS             40099..40836
                     /gene="fliP"
                     /locus_tag="ACHOSM_RS08265"
                     /old_locus_tag="pgaptmp_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HGU4558200.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVNAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            40846..41115
                     /gene="fliQ"
                     /locus_tag="ACHOSM_RS08270"
                     /old_locus_tag="pgaptmp_001654"
     CDS             40846..41115
                     /gene="fliQ"
                     /locus_tag="ACHOSM_RS08270"
                     /old_locus_tag="pgaptmp_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HGU4558201.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            41124..41909
                     /gene="fliR"
                     /locus_tag="ACHOSM_RS08275"
                     /old_locus_tag="pgaptmp_001655"
     CDS             41124..41909
                     /gene="fliR"
                     /locus_tag="ACHOSM_RS08275"
                     /old_locus_tag="pgaptmp_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HGU4558202.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            42199..42822
                     /gene="rcsA"
                     /locus_tag="ACHOSM_RS08280"
                     /old_locus_tag="pgaptmp_001656"
     CDS             42199..42822
                     /gene="rcsA"
                     /locus_tag="ACHOSM_RS08280"
                     /old_locus_tag="pgaptmp_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HGU4558203.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(42866..43054)
                     /gene="dsrB"
                     /locus_tag="ACHOSM_RS08285"
                     /old_locus_tag="pgaptmp_001657"
     CDS             complement(42866..43054)
                     /gene="dsrB"
                     /locus_tag="ACHOSM_RS08285"
                     /old_locus_tag="pgaptmp_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HGU4558204.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            43217..43444
                     /gene="yodD"
                     /locus_tag="ACHOSM_RS08290"
                     /old_locus_tag="pgaptmp_001658"
     CDS             43217..43444
                     /gene="yodD"
                     /locus_tag="ACHOSM_RS08290"
                     /old_locus_tag="pgaptmp_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HGU4558205.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            43742..44557
                     /gene="yedP"
                     /locus_tag="ACHOSM_RS08295"
                     /old_locus_tag="pgaptmp_001659"
     CDS             43742..44557
                     /gene="yedP"
                     /locus_tag="ACHOSM_RS08295"
                     /old_locus_tag="pgaptmp_001659"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HGU4558206.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(44554..46248)
                     /gene="dgcQ"
                     /locus_tag="ACHOSM_RS08300"
                     /old_locus_tag="pgaptmp_001660"
     CDS             complement(44554..46248)
                     /gene="dgcQ"
                     /locus_tag="ACHOSM_RS08300"
                     /old_locus_tag="pgaptmp_001660"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HGU4558207.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(46419..46601)
                     /gene="yodC"
                     /locus_tag="ACHOSM_RS08305"
                     /old_locus_tag="pgaptmp_001661"
     CDS             complement(46419..46601)
                     /gene="yodC"
                     /locus_tag="ACHOSM_RS08305"
                     /old_locus_tag="pgaptmp_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HGU4558208.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(46680..47597)
                     /gene="yedI"
                     /locus_tag="ACHOSM_RS08310"
                     /old_locus_tag="pgaptmp_001662"
     CDS             complement(46680..47597)
                     /gene="yedI"
                     /locus_tag="ACHOSM_RS08310"
                     /old_locus_tag="pgaptmp_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HGU4558209.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            47770..48690
                     /gene="yedA"
                     /locus_tag="ACHOSM_RS08315"
                     /old_locus_tag="pgaptmp_001663"
     CDS             47770..48690
                     /gene="yedA"
                     /locus_tag="ACHOSM_RS08315"
                     /old_locus_tag="pgaptmp_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HGU4558210.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGLRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(48679..49149)
                     /gene="vsr"
                     /locus_tag="ACHOSM_RS08320"
                     /old_locus_tag="pgaptmp_001664"
     CDS             complement(48679..49149)
                     /gene="vsr"
                     /locus_tag="ACHOSM_RS08320"
                     /old_locus_tag="pgaptmp_001664"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HGU4558211.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(49130..50548)
                     /gene="dcm"
                     /locus_tag="ACHOSM_RS08325"
                     /old_locus_tag="pgaptmp_001665"
     CDS             complement(49130..50548)
                     /gene="dcm"
                     /locus_tag="ACHOSM_RS08325"
                     /old_locus_tag="pgaptmp_001665"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HGU4558212.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(50615..51310)
                     /gene="yedJ"
                     /locus_tag="ACHOSM_RS08330"
                     /old_locus_tag="pgaptmp_001666"
     CDS             complement(50615..51310)
                     /gene="yedJ"
                     /locus_tag="ACHOSM_RS08330"
                     /old_locus_tag="pgaptmp_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HGU4558213.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYCHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLAHVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(51350..51631)
                     /gene="drpB"
                     /locus_tag="ACHOSM_RS08335"
                     /old_locus_tag="pgaptmp_001667"
     CDS             complement(51350..51631)
                     /gene="drpB"
                     /locus_tag="ACHOSM_RS08335"
                     /old_locus_tag="pgaptmp_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HGU4558214.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT        12857 a        13447 c        12780 g        12856 t
ORIGIN      
        1 gttgaaatca tatgatcacc gagagtgatt gattaattgt ctttactaat aatcagacta
       61 atatttacct gtttgactga gttgggattg cgtcgtttct ccattaggag taaagcttta
      121 atgtcacctg aagttcacag aataaggaac aggttatgag ctatagcaat attcttgtcg
      181 ctgttgcggt tacaccggaa agtcagcaac tgctggcaaa agcggtatct atcgccaggc
      241 cagtaaaggg acacatcagt ttaattactc tcgcttccga cccggaaatg tacaatcaat
      301 tagctgcgcc gatgcttgaa gatttacgca gcgtgatgca ggaagaaacg caaagctttc
      361 ttgataagtt aattcaggat gcggggtatc ccgttgacaa gacgtttatt gcctacggtg
      421 agttaagcga acatattctg gaagtttgtc gcaaatatca tttcgatttg gtgatttgcg
      481 gtaatcacaa tcacagtttc ttttcgcgag catcctgctc ggcgaaaaga gtgattgcct
      541 caagtgaagt cgatgtgctg ttagttccac ttacgggaga ttaaccgctc ctacgcaagc
      601 ttaggaaagg tagcaacttt atcgcgctgc tggctttccg cgcttcgcgg aactatctgc
      661 tttaggtcgc tgatgaagca ctcctgccag tggttaatat cgtttttcac gataacgtcc
      721 agcatttctg catgacggga aatacgttcc gccagcgaca tagtcaatgc acgatccagc
      781 gcagctgcaa cttcgtcacg atcgtagggg ttaacaatta acgcggacgt taactcgttt
      841 gccgctcccg caaattgcga aagaacaaga acgcccggat tggctggggc ctgagcggca
      901 acatactctt ttgccaccaa gttcatcccg tcacgcagtg gcgtcactaa gcccacgtca
      961 gagtagcgga atattttcat cagtaattta cggtcaaaat gctgattcaa ataataaaga
     1021 ggcgtccagc ctaattgccc gtatttacca ttaattcgtc cagcttcatt ttcgagctga
     1081 tgacgaatat cctgataggc ttgcacatca ccacgcgacg ttggtgcaat ctgggtataa
     1141 cgaattttac catgatgctg cggatatttt tccagcaacg cttcataggc gagaaaacgc
     1201 tctggcaaac ctttggaata atccagccgt tcgacagaaa agatattttg tacgtttttc
     1261 agttccgctt taagttgcgc cagttttggc ggcagtggcc cggcagcctg tttggctatt
     1321 tcttttggct caatgccaat cgggtaaact tctgttcgaa acgctttgcc ccaggctgta
     1381 tggctttttg cgctacgtgt cgtgacgcgg gtcaggttag aaaggcaatc caggaacgcc
     1441 agacgatcgt tttctgtctg gaaacccagc aaatcataat cacaaagctg ttcaagcaag
     1501 gtgtcatatg tcggcagcgc gttgaagatt tccggtgtcg ggaaaggaat atgcagaaag
     1561 aaaccaatgc gattattcac tccccgtttg cgtaattcat gcgcaaatgg caacaggtga
     1621 taatcgtgga tccagataat gtcatcgtct tgcaacagcg gcagtaattt atctgccagc
     1681 aacgcattta cgcgtagata gccgtcccag gcaggacgct gaaattgcac cagatcgagg
     1741 cgataatgaa aagcaggcca gagaacggca ttggagaatt ggttgtagta ttcgtcaagg
     1801 tcctgttcgc tgaggttaaa agaggcccac gtaatgttac cctttttcac cttttttagc
     1861 ggctgatcct cattccctgt ttcaccactc cagccaaacc acagtccgcc tgcggctttc
     1921 agtgccccca gtatgccaac ggcaaggcca ccggcactgg cggcgtgctc atctggtggt
     1981 gcaatccggt tagatactac gactaaacga ctcatagtca tcactcctgt tattttctct
     2041 tttttgttgt aatgcggtgg ttatcatttc aagccagctc cagacatccg gtacgcccgc
     2101 cagtcgccat gatgcctgag ttgcgcctgt gccaattttt actgacattc cgcccagtcg
     2161 gttaacgact gcgaagccag attcatcggt taaatcatcg cccagaaata cgggagttcg
     2221 cccgataaag ggagcttcct gcataaaggc agcaatcgcc tcacctttac tggtacctct
     2281 cggtttgatc tcgacaacac actttccctg ctgtaacgcc atttgtggcc agatctgagt
     2341 aatacgttgc gctaatgtca ttaatgcgtc ttcatgctgc ggagcctgac gataatgcag
     2401 cgcaaaagcc atccctttcg tctccagctc cgcaccggga tactgtgcga tgaccgtatg
     2461 cagttgcacg ttaatatcac gtgcaatcgc atccggcaga tgaacgatat gcgttttacc
     2521 attgatgtca cggcgctccg ccccatgcac gcccgctaac gggaagcgat aaggttttgc
     2581 cagtgcgtcc agctccacca ttgagcgccc tgatatcaat gccaatgcac catcacttgc
     2641 ggttgccagt agctgaagtc cttgcagaat attgtcaggt acgacgacct gatcgggatg
     2701 cggtttgatt tccgccagcg ttccatcgag atcaaaaaac caggcatatt tcgcggatag
     2761 ttcaggggtt tcggttaacg gttctgtcac ccggttctcc tccttctctt ttcttattgt
     2821 tttgcgcaga acgcgcttta cagacatgta agtatagaca gtgtgacggg ggtcgccatt
     2881 tgcaacgaag aacagccaac cgttgtggcg gctggctgtg gtgggaaaaa acgttaaatt
     2941 gttgtggaaa aaagcatcag acagtgcgtt tcgctttttg cttgtaacgg tcgaagatca
     3001 ccgctgccag caggattaag ccgcgaacca cgtactgcgc gaaaggagaa atattaagca
     3061 ggttcatggc gttctccacg gtgcctaaaa ttaagatacc cgccaccaca tatgagattt
     3121 ttccgatgcc gcctttcaga gaaacgccac ctaaaacgca ggcggagata acaataagct
     3181 cataaccaat cgacgtcatt ggctgcccac tggtcatacg tgaagcgaga ataattccgg
     3241 ctatcgcaga taccaggcca gagagaacaa agataataat tttggtgcga acaaccggta
     3301 cacccgccag acgcgcagcc tcttcgttcc cgccaatcgc cagggtatta cgaccaaagg
     3361 tggttttatt cagcagcaaa ccaaagataa tcagacacgc gacggtgagc cagattggcg
     3421 caggcagacc gaaccagtta gcgtaaccaa gggcaaagaa gctttcatct tcgataccga
     3481 ccgctttacc gtctgaaatg atgtacgcca gacctcgaac aatctgcatc gttgccagtg
     3541 tcgtgatcag agcatttatt ttcagtttgg cgataacaaa gccattgacc aggccacaga
     3601 gaatgccaag cagcaacccc gctgccacgc caatccacag gctttcagtc aggttgataa
     3661 ccaccgccgt ggtgacaccc gcacaggcaa ttacggaggc gacagaaagg tcaaagtcac
     3721 cggaagcaag gcagaacaac atgccacaag ccaccatccc cgacatggaa attgccaggc
     3781 ccaacccttt catattaatg aaggtggcaa agttcgggac aaaaatggca caggcgataa
     3841 aaagcaccgc aaacaccacc agcatgccgt actgatccca gatacgcccg aagctgaatg
     3901 acgacttagg tgcgccagaa cccgatgtag aaacagaaga catcatactc tccttactca
     3961 ggcaactgcc tggctgactt taggcatcgc aaggctcagt gcctgacgct catctgcctg
     4021 ctcgtgtaac aattcaccgg cgatttcacc ttcccgcatc accacaatcc ggtcggcaac
     4081 gccgaggact tcaggtaagt cgctggaggc aaacagcacc gccacaccct gcgccgccag
     4141 cgcataaatt acgttatata tttcgtgctt agcaccaaca tcaatgccgc gcgtaggttc
     4201 atccagcaaa atgaccttca tctcttccga taaccagcgg cccagaatgg ctttttgctg
     4261 atttccgcct gagagattca tgatcagttg ctccgcgccc ggcgttttga tgttgagcga
     4321 acgaatgtgg tgatcggcat tgttttcttc ccaaccgttg ttgattacac aaccgccgag
     4381 cacatgttta cgtctggcac tgatgttgat attgtcgcga acggagtgca cgggaataat
     4441 gccttccgct ttgcgatctt ccgggcagag catcatgcct gcggcaatgg cgtggctcgg
     4501 tttacgaata tcgatcggct gttggtcgat ataaacctga ccggcggtga tttgcgtccc
     4561 gccaaacatg cctttcatta attcgctacg ccccgcccct accagaccaa acagcccaac
     4621 aatttcacca ctgcgaaccg ccagacttat tggcgtacgc acgcctggtg ctttcacagc
     4681 atcaagacgc aggcgctcct cgccataact acgcggttgc cagccgtaga tatcgccaat
     4741 gtcgcgcccg accatcgcct gcaccagcgc gtcgtggtca acctgctgca tatcggtaaa
     4801 ggttttgaca taacgtccat ctttaaagac agtgatggca tcgctgaggg caaatatttc
     4861 ttccatacgg tgagaaacgt ataagatcac ccgcccctct tttcgcagtt cacgaataac
     4921 gcggaaaaga ttgtctattt cgcgggcaga aagggagctg gttggctcat caaaggcgat
     4981 aattttagcg ttacgcgcca gcgccttggc gatttcaacc atctgccact gaccaatgga
     5041 gagatatttc agcggcgtgt ccgggtcaat atccatacca agatgtttaa gttgtaaacc
     5101 cgcctcataa ttcagcaatg agcgattcac aatgccgcct ttatgcggca gctggccgag
     5161 atagatgttt tccgcgacgg tcatttccgg cacgagatgc agttcctggt aaaaaatcgc
     5221 cacacccgcg ttaagtgctg cggtcgtgtc ggaaaaggac atttcctgcc cattaatcac
     5281 tacagaaccc gtggttggcg catagttgcc gctgaggatt tttaagagag ttgattttcc
     5341 tgcgccattt tcacccatca acgcatgaac ctgaccggca tagcagtcaa aactaatatc
     5401 cgtcagcgcc ttaacgccgg gaaacgtttt accgatgccg cgaaatgaga gatacggggt
     5461 agactgttgc ataacgtctc cgtgaatcac tggtcgttca caccccctct gcatcatgca
     5521 gaggggaatt tttccggcaa attacttatc gcctaaacct tttttctcca attcttcttt
     5581 aaagttgtca cgcgtgatca gcaccacgtc ggtaacttcg gtgaatttgg tcggttcaac
     5641 accttttgct acccagttgt aaagcatttc gctggactta taaccatgta cgtccgggct
     5701 tggcaacagg gaaccgtaga agccggttgc ctgtgcctta gacagttcgc tcaccgcatc
     5761 cacaccgtta ataccgatac cgatgatatc ggccgcttta aagccctgac cttccgtcgc
     5821 gcgtacgccg cccagcacgg tgctgtcgtt cataccgacg atcagccaat gtttaacttc
     5881 cggatgttga accagcattg agttggcagc gtcaaatgcc ccggggatgt cgttagattt
     5941 ggtaggtacc tgataaattt gtttttccgg gaatcctgca gctttcagcg catccataga
     6001 acccgtagta cggcgacgag ccgtatccag ttcgttggag gtaatcgcca tcaccgcgct
     6061 ttctttgaca tcccagccac gtttctgcat ctctttatac agttcctggc cctgacgttc
     6121 gccaattttg gtcgccgcca tcatcaccag aggaacggta tccattggct tacctttggc
     6181 gttaacaaac tggtcatcca cggcaatgac tttcatatcg tagccacgcg ctttcgccac
     6241 gatggcagag ccgagtttcg ggtccggagt acaaataacg aaaccttttg cgccactggc
     6301 ggccaggcta tcgatcgcgt tcaatgtttt ttcgccatcc ggcacggcaa tcttaataac
     6361 ctcaaaccct aaatccttcc cagctttatc ggcaaacttc cattcggtct ggaaccacgg
     6421 ctcttccggt tgcttcacca ggaaaccgag cttcaggttc tccgccatag cggattgtga
     6481 cataacggct gccagaccaa tggctgccag ggctttagta aatttgtgca tggttctctc
     6541 cagctttagt gtcgttttgt gtagggcaaa aacgaatgac attcgttaaa ttaatcggaa
     6601 aacaaagcat taccttttaa ctaaaagata agtgactgtg ttgacatagt tttagcgaga
     6661 aattaattct ccataggaga gcaatatcac atcgcagaat tacagtgaga acgtgcataa
     6721 atttagcggg aaaagacata agggaaagcc aatttgtcag acaaattgtc gaatgcacag
     6781 cagattaatc cataagatta gcctggaaat ccttgttgtc tttggtaccc atgcgggatg
     6841 tcttcttttt aaccagtcaa taggccgcat tacctgacgt tgagcttttg aaatggcgta
     6901 ataaccgcaa ctcaaagatg tgggaaatgc gcgtcattca tttcgccatt aattatcacc
     6961 gtgctcatta attaacagaa cacgtataat gagagccatc tcgcaaaaat gaaaaaacgt
     7021 tttataaaat catcacttca tcatgaattc aaattcattg attaatatca acaagataca
     7081 aaaagcacta tcattaaaat tcattgcagt tacattgatt tcatcaatga aatgtaaaaa
     7141 tatataaact tgatgattta agcattttct tatacccgtt cagacgttat tcttatttca
     7201 gatcatcgtc agaattgact ccacgatcac atttcggacc ggcagaaagg aattattctg
     7261 caaacagtaa ttatggtgtt ttgatttatc ttgcacctct ccacttctgg atataaggat
     7321 attaggtatg gcaaccgctg gaatgcttct caaactcaac tctcaaatga accgcgagtt
     7381 ttacgcatcc aatctctacc ttcacctgag taactggtgt tctgaacaga gtctgaacgg
     7441 caccgccact ttccttcgcg cccaggcaca gagtaatgtg acccaaatga tgcgcatgtt
     7501 taactttatg aagagtgtcg gcgctacccc catcgttaaa gccattgatg ttcccggtga
     7561 aaaactgaac tctctggaag aactgttcca aaaaacgatg gaagaatacg agcaacgttc
     7621 tagtacgttg gcacagttag ccgatgaagc gaaagaactg aatgatgatt caaccgtcaa
     7681 tttcctgcgc gatctggaaa aagaacagca gcatgatggc ctgttgctgc aaaccattct
     7741 tgatgaagtg cgcagtgcga aacttgcggg tatgtgccca gtgcagaccg accaacatgt
     7801 tctgaatgtc gtgtcgcatc agctgcattg atcatacatc ggcgctaatg cattgcgccg
     7861 atgaagtttt tgagaaaccg ctgcctcatc tttttgaagc agcggttttt taatgggatt
     7921 cgccctgtgg ggtaaacttg agttcaatca gcgcgatggc tttttggatt gcccgcatgg
     7981 tgaccgggtc tgcggcggcg ggatggttag taaagtcgat attcttcagc tgactggaca
     8041 ttttttcacg aacttcaacg ggcgcgatta catcgagaac atccagaatt tgtttgataa
     8101 ccaactggca agcaaccaca tcagaaacca gttcctgatc ggcattcagc ggctgggaca
     8161 tcgtaaactc ctgatagcat tttgaaagcc gttatagtag cggcttcaca tcttcagcga
     8221 tagtcacatc caccgtcatc aggacacaaa aaaacctgcc ggagcaggtt ttttgttatc
     8281 ggaacatatt gcctggcggt acgtctttga acgtcttgca atagttattg aacatacttt
     8341 tcaggatttt gcgcagtttc atcgcggcac tccgaccatt tgttatacag gtgttattgt
     8401 ctttgcgctt ataatatgac aaccatcaca aaaatcaatc tttatgtgat acaaatcaca
     8461 taaatacccc tttaatgtta taaaaatgat aatcaaaaaa cagcccccct atttctgaca
     8521 cctacagatg gcaagaaata gcgcctgcca ggcgtctttt ccggccattg tcgcagcgct
     8581 gtaacgcgta aaatagtgct ttctcttact cttctggctg gaccatgaga cttctgattc
     8641 tgactctttc attaataacg ctggcggggt gtacggttac tcgccaggcc catgtgagcg
     8701 aagttgacgc ggcaacaggt attgtacggt tggtttatga ccatgctttt ttgcagcatg
     8761 cccataccga tccttatgtc agtcgcggga tcgccgatcg cgcatgccag caggaaggct
     8821 atacccacgc gatccccttt ggtcagccag taggtaactg tagccttttt acgggttcct
     8881 tatgtttgaa tacggaattc actttgtcat atcagtgcca tcattccgcc ttccctgtct
     8941 ttctctaaat attttaatca gcgaggggat cttcgctgat taaagaaata gcggtaatgc
     9001 gttttattcc cattcgtatt tttaataatt gaagtttata ttttaccttt tgcaaataat
     9061 aaaataacaa attatagtga cgccacagca acaaatataa cctttgtgga gcactatcat
     9121 gctgaaacca gaaatgattg aaaaacttaa tgagcagatg aacctggaac tgtactcttc
     9181 actgctttat cagcaaatga gcgcctggtg cagctatcat accttcgaag gtgctgccgc
     9241 gttcctgcgc cgtcacgccc aggaagagat gacgcatatg cagcgtctgt ttgattacct
     9301 gactgatacc ggcaatttac cgcgtattaa taccgttgaa tctccgtttg ctgaatattc
     9361 ctcacttgat gaattattcc aggaaaccta taaacacgaa caattaatca cccagaaaat
     9421 taacgaactg gctcatgctg caatgaccaa tcaggactac ccaacattta atttcctgca
     9481 gtggtatgtt tctgagcagc atgaagaaga gaaactgttc aaatcgatta ttgataaatt
     9541 aagcctggca ggcaaaagcg gcgaaggtct gtattttatc gacaaagaac tctctaccct
     9601 cgacacacaa aactaatgct ctccggcggc aggtttctgc cgccgtttca aatcagtgac
     9661 ggcaaatctt actctgatcg gtcgaaaaac cttcttctga aggtataaat ttgccttttg
     9721 ctgccagaaa cgctaccagc tctcccgctg tcatcccttc tgccgagcaa gtgtgaaaac
     9781 gtgcctgttc accaaaacgc gctttaatcg cagcttccag actggcatgc gtatattgct
     9841 cgcctgattc aatcatcata tttaacactt catgaccgtg aatagagtcc atcgttcctc
     9901 ctcaaaaaaa agcctagcgt agcgattacc gcttatgaag actttgcgcc agcgcaggac
     9961 tgaatgcttt ttattgtaca tttatattta caccatatgt aacgtcggtt tgacgaagca
    10021 gccgttatgc cttaacctgc gccgcagata tcactcataa agatcgtcag gacagaagaa
    10081 agcgtgaaaa acagaaccct gggaagtgtt tttatcgtgg cgggaaccac aattggcgca
    10141 ggcatgctgg caatgccgct ggctgcggcc ggtgttggtt ttagcgttac gttaatcttg
    10201 ttgattgggc tttgggcgtt gatgtgctac acggcgctat tactgctgga ggtgtaccag
    10261 catgttccgg cagataccgg tctgggcacg ctggcaaaac gctatctggg acgctacggt
    10321 caatggctga cgggcttcag tatgatgttc ttaatgtatg ctctgactgc ggcatacatc
    10381 agcggtgccg gtgaattgtt ggcctccagc atcagcgact ggacaggtat ttcaatgtcg
    10441 gcaaccgctg gcgtgctgtt gttcactttt gttgccggtg gcgtggtttg tgttggaaca
    10501 tcgctggtcg atttatttaa ccgttttctg ttcagcgcca aaattatttt tctggtggtg
    10561 atgctggtat tgctgctacc gcatattcac aaagtgaatc ttttaaccct gccgttgcaa
    10621 caggggctgg cactgtctgc aatcccggtg atttttacgt cgtttggttt tcacggtagc
    10681 gtgccgagta ttgtcagcta tatggatggc aacattcgta agctacgctg ggtgtttata
    10741 accggtagtg cgatccccct ggtggcatat attttctggc aggtggcgac gcttggcagc
    10801 attgattcaa caacctttat gggattgctg gctaatcatg ccggattaaa cgggctgcta
    10861 caggcgttac gcgaaatggt ggcctctccg catgttgagc tggcagtgca tttatttgct
    10921 gatttagccc tcgccacgtc atttctcggc gttgcgttag gcttatttga ttatctggct
    10981 gatttgtttc agcgttcaaa taccgttggt ggaagattgc aaactggtgc aattaccttt
    11041 ctgccgccgt tggcgtttgc actgttttat ccacgaggat ttgtgatggc gctgggttac
    11101 gccggtgtgg cgctggcggt actggcattg attatccctt cgctgttgac ctggcaaagc
    11161 agaaagcaca atcctcaagc gggttaccgg gtcaaaggtg gtcgtccggc gctggtggtg
    11221 gtgtttctct gtggtattgc tgtgattggc gtgcaatttt tgattgcggc agggttgtta
    11281 ccagaagtgg ggtgattaga tagcctcaaa ttccttattg ggtgccagaa ttaacgctga
    11341 cacccaattt gacttcttaa tgcaggcagc actgcttaaa tttcttacca ctaccgcacg
    11401 ggcaaggatc gttacgccct ggtttctctt ctgctttgat cggttgctga acagcttttt
    11461 cctgcggatg cgccatccag tacgcatgta gatcaagcgc cgccagtcga atggcatcta
    11521 cgctctcttc aaacgcttct ggcgacatct tctctacccg ctcgaagttt tcctcagtac
    11581 cgtgcagcgc aatcgcctcc agcgctggtt ttaacgaatc gggcaatgtt gaccagtcag
    11641 aaagtgccac gccccgcata tagccaaagc accactcctc aacaatcgtc agctcgctgc
    11701 catcaacttc tcgcaagccg aataacggct caaactgctc cgggaattcg ttcagacgct
    11761 ctgcggtatc ggccatatgt tgaaaagcca ggttcataaa gcgcgtcatc tctttctctg
    11821 acgcccagcg cggcacatag tcagccccac cccacacggc aaccagccac tgtgccggtt
    11881 caatctcttg cggagaactc aacaccgccg tcaataaacc gtccagctcc gccacatcaa
    11941 ggatggcgtg gtcagtgttg tatttggtca gattatcgtc cagccattcc aactcacttt
    12001 cgtttaacgg tcccgttttc atacgctttt ccttgtggat ctcaactcgc cagcacctat
    12061 cttacatgcc ggtccgtatc agagatactt tttgagtggc tttgccgatg attaaaaatt
    12121 aaggagggtg taacgacaag ttgcaggcac aaaaaaacca cccgaaggtg gtttcacgac
    12181 actgcttatt gctttgattt tattcttatc tttcccatgg tacccggagc gggacttgaa
    12241 cccgcacagc gcgaacgccg agggatttta aatcccttgt gtctaccgat tccaccatcc
    12301 gggctcggga agaaagtgga ggcgcgttcc ggagtcgaac cggactagac ggatttgcaa
    12361 tccgctacat aaccgctttg ttaacgcgcc aaattcttca ggcctttcag ccagacatcc
    12421 gctgacgccg atgtctttta aactggagcg ggaaacgaga ctcgaactcg cgaccccgac
    12481 cttggcaagg tcgtgctcta ccaactgagc tattcccgca ttcatcaagc aatcagttaa
    12541 tcacttgatt ttattatcgt ctggcaatca gtgccgccgt tcgatgcgtt gcattctact
    12601 tacctggcgc gatgagtcaa cgatattttt caccactttt gatcgtttgc tgaaaattgc
    12661 gccgaaacga tcactgatca agcaaatctg cacgcgcagc gctcaaatat tgcaacattg
    12721 accacagagt cagtaccgca gccacaaaga aaagtgcaat accggcgtac tcaacccaaa
    12781 tgttcggacg ccacagcagc catgccaacg ccaccatctg ggcagtggtt ttcactttcc
    12841 caatccagga gacagccacg ctactgcgtt tacccaactc cgccatccat tcgcgtagcg
    12901 cagaaataat aatttcacgg gcgatcatcg ttgccgccgg taaggtcacc caccagctgt
    12961 ggtaatgctc ggttaccagc accatggcga tagccacgag aactttatct gctacagggt
    13021 caaggaaagc accaaaccgg gtactctggt tccagcggcg tgccagaaaa ccatcgaacc
    13081 agtcagtcac cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac caggtgacag
    13141 gcagataaaa gaccaataca aagaatggga taaggatgac acggaacagt gtaagcaacg
    13201 tagggatatt aaattgcata atgacgggta actatctgtt gtcagtaaga ttacccctat
    13261 gttgctacag agacatcaat gtttcaacga ccagaagatc ttttctgcca gaccttgcga
    13321 aatacccggc acttttgcaa tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc
    13381 catatatttc aacaacattt gccggcgttt tggcccgacg ccttcaatgg tttccaggga
    13441 actggtattt ttgaccttcg cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga
    13501 ttcatcccga atatgctgga taacatgcag cgcgggagaa tctggcggca aactgaatcc
    13561 ctcaccttcc ggctcaaaga acagcgtttc cagcccagcc ttacgatctg ctcctttggc
    13621 aacgccaagt agcagcggat gatttttatc ccatgagaca tccagttcgg cgaagacatt
    13681 tttcgcctgc gcaagctggc ctttgccgcc gtcgataagg atcacatccg ggatcttact
    13741 gtcgtcgatg gctttaccat aacgccgacg cagcacctga ttcatcgccg cataatcatc
    13801 gcccggcgtg atgccagtaa tgttatagcg ccgatactcc gcacgcagcg ggccgttagc
    13861 atcaaacacc acacaggaag cgacggtttg ttcacccatg gtatggctga tgtcaaagca
    13921 ctccatccgc ttcacttccg gcaatttcaa cacgctggca agtgctgtaa gccgctggtg
    13981 aacggtagat tgctgcgaaa gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag
    14041 tttcaggtaa cgcgccctat cgccacgagg tttggtttga acattaatct tgcgtcccgc
    14101 cagttctgaa agggaatcgg cgagcagcgt tttatcgcta agattaaaat cgagcaggat
    14161 ctcacccggt aaggtgcgca tctggctgcc ttgtaaatag aactgtccta cgaaggtttc
    14221 caccacctcg ctcagttccg taccgccagg cactttcggg aaatagctgc ggctgccaag
    14281 cactttgccc tgacgaatga acaatacgtg gacacaagcc atgcccgcat cgaacgccac
    14341 accaataacg tcaaggtcgt cgccggtatt ggacacaaac tgtttttcgg tgacgcgtcg
    14401 caccgcctga atttggtcgc gaatacgtgc cgcttcttca aactccagat tctggctggc
    14461 agtttccata cggctaatca gttgcgtaag cacctgatca tctttgccag acaaaaacag
    14521 gcgcacatac tcgacctgct gagcgtattc ttcttcactc accagtcctt caacgcacgg
    14581 tcccagacag cgccctatct gatattgcag acatggacgc gagcgattgc gataaacact
    14641 attttcgcac tggcgaatgg ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc
    14701 atagccattc gggaacgggc cgaaatattc acctttggca tgctttgcac cacgatgcat
    14761 cgccagacgc gggtgggtat cgccactcag gaagataaaa ggatatgatt tatcatcgcg
    14821 tagcaaaacg ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca gcaacagcgc
    14881 ttcggtttct gtatgagtaa ccgttacatc aatttgctgg atctgggcga ccagcgcttc
    14941 ggttttgcgc gaagcgaggt tgctacggaa atagctggaa agccgttttt tcaggtcttt
    15001 cgctttgccg acatagataa ccgtaccacc agcatcgtac atgcgataaa cgccaggctg
    15061 gctggttacg gtttttaaaa acgcttttgc gtcaaactga tcactcactg acttgataat
    15121 gtctccgcat tacacagacc atggcgaatt gccaggtgag tcagctcaac atcgccatga
    15181 atgtttagtt tactgaacat tcgatagcgg tagctgttca ccgttttcgg actgagattg
    15241 agctgttctg agatctcatt gaccttctgg cccttcgtga tcatcagcat aatctgcaat
    15301 tcacgttcag acaaactggc aaatgggctt tctgtttttt ctggttcgat ctggcttaat
    15361 gccatttgtt gagcgatgtc agaagcaatg taacgctgcc cagaatagac agaacggatc
    15421 gcactcacga cttcctgcgg agccgcgcct ttgctgaggt agcccgcagc accggcctgc
    15481 atgactttcg ctggtaaagg gttttctgta tggacggtaa gcatgatgat tttgacatca
    15541 gctgtggaac gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg catactcatg
    15601 tccattagca ccacgtcaac ggcatttgcc cggcaccact taacggcgtc ttcaccgcac
    15661 gatgcctcac cgacgacttt tatacccttt atatcttcca gaatgcgtcg tatccctgcg
    15721 cgcaccagtt cgtggtcatc aacaagtaga acgttgatca aaggaatatc tccagaaata
    15781 gggataacgc tactgatagt tagtcattcg tatattagcg gtttttattg caactttgaa
    15841 acgttaaaaa tggtgggtat tcgatttttc tctcgttttt gttcattcga ttgtccacag
    15901 ttatggtcac gcccgtcacc aacggcttac cggatcgttt cacagtaagc cctgcgacgt
    15961 aatgtgacaa aaattacctt tattcagcaa aaatgaaaat cagcccgctg aatatgtaac
    16021 attaattaac ccgaacaata cgggcaaaaa acattcatga taaaaatatt tattggtcat
    16081 tatattaacg catttaatag caccgttgac gacacgctca aaaaacaacc actgctattt
    16141 ttagcaaagc ttatggtata ctccgccgcc ttaacatttt tcaccgcaaa ttttcattgc
    16201 aacatgacga ggaaaataaa tgagtacgcc tgatttttct actgccgaga ataatcaaga
    16261 actggcaaat gaagtctcct gcctgaaagc gatgctgacg ctgatgctgc aggcgatggg
    16321 acaagctgac gcgggccgcg tgatgttaaa gatggaaaaa cagcttgcgc tgatcgaaga
    16381 cgaaacccag gctgcagtat tttccaaaac ggttaagcaa attaaacaag cctaccgtca
    16441 gtgatataaa accggctgat agcgtgcctt tcagccggtt tttgcatctg gcacgcagga
    16501 cagaaaagag atcaaattaa gccagtagcg gccgcgtaac aggcaacctg ggtcttattt
    16561 ggtgcattaa tttttttctg catgtttttc tggtggaaat tgaccgtatt ctccgagatt
    16621 gacaaaatca tcgctatctc tgctgatgtc ttcccttccg ccgtccacct cagaatttct
    16681 ttttcgcgct tgctgaaatt catctctggt gtcatcacta tttcatcatt taaccgcatc
    16741 agagccatca gactttcgcg caccagtaac tgcattttta attgcagctc atcactaaga
    16801 atgggtattt cgcgcgcgct gcaacgggaa aaggacaaaa agcccagccc ccggtttggc
    16861 agcattaaat actgagtgac accgcggcgt aaaccatgtg cgcgcgcggc ttcccataac
    16921 ggctgtgctt cgctgaataa gtcatcattc cacattaaat ggccctgact aaagttttca
    16981 gggttcagca ccggatcaat tgcgagaaag ttttttgcct gataataact aacccacgcc
    17041 tcagggtaat tggtgtaaaa agccacttta ggtcgcgtga atggtaccgg gtggcgaaca
    17101 cataacgaat agtaatcgta ctccagcagc tgagcctgaa gctctatttc atggtagacc
    17161 tcttctgcgg cctccatcct ctgaaaacgc aacagcatcg tgcgacgcca gctgaaaaaa
    17221 tccgtatcct gcatagtaaa ccgcaacgcc cctgagagtg agtatcattt ataatgataa
    17281 ttcaaactat cacataaata agatttatat ataatttata ttattcaggc aatgaattac
    17341 ttttgcaagc catcacattc tcttatgtta ttaatgagtt atgctgattt gttaagcagt
    17401 tttatcaggc ttgaaatggc gttccgcccc gactgtcgaa tcgtcggggc tgattttttc
    17461 ttattattgc agcagaaact tctcgaggaa ctggcgggtg cgaggctgct cgggatcggc
    17521 aaataacgct tttgcggccc cctgctcgac tatccgcccc tggtccataa aaatcgcccg
    17581 gtccgcaaca tcccgggcaa agctcatttc gtgtgtcaca atcaccatcg tgcgcttttc
    17641 ctgcgccagc tgacggatgg tgttcaggac ttcgcccacc agctctggat ccagcgccga
    17701 cgttggctcg tcaaacaaaa tcacttcagg acgcattgcc agagcacgcg caatcgcaac
    17761 acgctgctgt tgaccgccag acaaacgacg tggatagctg gtttctttac cggccagccc
    17821 aacttttgcc agtagctcgc gagcgcgcgc cgtagcctct tcttttggtt cgcctttgac
    17881 gatcaccggc ccttcaataa tgttttccag caccgtacga tgcggaaaca aattaaagtt
    17941 ctggaagaca aacccgacgt gctgacgcaa ctggcgaatc agcgattttt gctgacttaa
    18001 tgaacgagca gtatcaatag tgatatcgcc aacggtgatc gttcccgctt ccggttgttc
    18061 cagcagattt atgctacgta gcaacgtggt tttgccggaa ccactcggac cgataattgc
    18121 caccacttcg ccaggcttta cctcaaggtc gataccgtgc agcaccgtct gaccgtgaaa
    18181 ttttttcacc aggttcttaa cttcaatggc actcattttg gttctctctc ctggcgatta
    18241 agttgattct caaaatggtt ctgcaacgtc gataatactg tcgccatgat ccagtagatg
    18301 agcgaagccg ccaggtacat ggtgaacact tccagcgtgc gcgaggtaat caactgcgcc
    18361 tgacggaaca gctccggcac ctggattgtc gcggccagcg acgtatcttt taccagactg
    18421 ataaagctgt ttgacagcgg cggcaacgcc acccgcgccg cctgtggcaa aatcgcgcga
    18481 cgcatagtct gccatggcgt cataccaata ctggcagctg cttcccactg acctttatca
    18541 atagaagaaa tggcggcgcg aagcgtttcg gcagcatagg cggcagtgtt gagcgacaga
    18601 ccaatcattg ctgacggaat gggatctaat tcgataccaa actgcggcag gccgtaatag
    18661 atcataaaca gttgtgcgat gagcggcgta ccacgaaaaa tagagatata aaaacgcgcc
    18721 agccagcgca cgggccagat tggcgacagg cgcatcagcg cgagaataaa cccgagcagt
    18781 aagccaaaaa acatgccgcc gatactgagt tgcagcgtat acccagcccc tttcagcagg
    18841 aacggcaagg aatcaataac cagttgtata ctttcttgca tgagcgtttc cggagctaaa
    18901 cgtggggatg ataggcgaac agcgcaggcg cgccgccggt atgaataaac agaatcggcc
    18961 cttcatcttt gaagcgtttc tgactgatac cgtcaatcag ccccgccatc gcttttccgg
    19021 tatacacagg atcaagcaga atgccttcca gccgcgccag caatttcact gcttccatgc
    19081 cttcatcgtt cggcacgccg tagccaggtg caaaatagtc atcccagagt aaaatttccg
    19141 ctgatgcggt cagctccagt tctttcgcaa tcgcctgttg taggttaacc actttcggca
    19201 attgatcggc aacggaacgc gacacggtca cgccaatcag ttcgctttca ggcatcaggt
    19261 gttccagccc aacagccagt ccggcgtgag ttccggcact gcccgatgcg actaccaccg
    19321 acgaaatatt aaccgcccct tcacactgtt gcgcgatttc cagcgcactc tccacataac
    19381 ctagcgcgcc aagagcatta gaaccgccaa ccggaatgac atacgggcga aagccttgtg
    19441 cttcgactcg cgtcgccagc tcttccagtt gggcattggg atcggtcagt gcttcgcaca
    19501 tttcaatctg ggtattgaac agatccagca acaagcgatt gccgttggtt aaatagtttt
    19561 ctgcggttgt gccaatagga ttttccagca gcgccacaca gtgcagaccg agtttcgccg
    19621 caactgcggc agtctgccgc acatggttag actggatcgc cccggcagta atcagcgtat
    19681 cggcaccatc gcgcagagca tctgccgcga gaaattccag cttacgtaat ttattgccgc
    19741 ccattgccat gggggtgacg tcatcccgtt tgatgaaaat ttcccgtcca agataatcag
    19801 aaaagcgcgg cagatattcg agcggtgttg gcgcgccgat aaactccaac cgtggaaaac
    19861 gggttaaatt atgcagttgc ataacagcct ccgatgtgtg ttgttgtgat tttcttatta
    19921 tgcacgctga aaacgcgtaa ataaaaaagg cgctagtgaa agcgcccttt tttgtcatta
    19981 tgctgattat ttggtcacat cagcaccaaa ccatttttcg gaaagagctt gcagagtgcc
    20041 gtctttttgc atttccgcaa ttgcatcatt cactgctttc agcaggtcct catttccttt
    20101 acgcagcgcc acgccagact cctgacggga gaatgcttca ccggttactg ccagcgtatc
    20161 gttggttttc ttcaccagat ccagcgccgc cagacggtca acgaggatcg catcgatacg
    20221 acctacgcgc agatcctgat atttggtcgg gtcatcatca taggtacgca catcgacgcc
    20281 ctgaacattc tgccgcagcc actcttcata gttggtgccc agaccgacac ccactttttt
    20341 gcctttcaga tcagcagccg ttttaatggt gccttcgtta ccttttttca ccagcgcctg
    20401 aataccagaa atggtgtacg gggtagagaa atcgtatttt ttcttgcgct cgtcagaaat
    20461 ggtgacctga ttaatcacca catcaatgcg tttagaatcc agcgacgcca gcataccgtc
    20521 ccatttggtc ggttttagtg acgcctcaac gccaagatgt tttgccagct gttgggcaaa
    20581 ttccacttca aaaccggtta atttgccgtc atccccctga aaactgaacg gcggataagt
    20641 tccttccagc cctaccagca gcgtgccgcg ctctttgact ttattaagca gaccttcatc
    20701 tgcaaaactt ttaacgctca tgcccgcaac cagcgccacg gccatcacac ccatcaatgc
    20761 ctgacgtccc agatgtgcta atttcatatt caccccgaat gttgttatgt ctgtttgcag
    20821 tgtagagcca ttgtttgtaa acacaaaaac aactccgcta catcttattc ttatttaata
    20881 tatatcagaa gcaggcaggc tggaggattt ctgcacaagt ccgatacagg tttggcgctg
    20941 atagtgttga tacttccgta acgcgatgcg gtaattgttg gtgctggtgg tgggcagcca
    21001 cggggcgagg atttcatcaa ggaaaccgtc ctgcagctga tcgagggaaa tattttgctc
    21061 gtgtagatga tttcccaaac ggcgcaaacg aacgacatat tcacgcaccg tacctgggct
    21121 catttcagtt tgctcaaaaa gaaattgctt aaatccgata atatcgaaaa aatcactctg
    21181 cgttttgcaa tgtaaatcac cgcaaaatcg gcacaatgcc gcccatgatt gttgttccac
    21241 ttgccagcca ttttcatcaa gcagcgtgtc caggcgggaa atctcgattt tattcactat
    21301 tttgccgtcg cgaacgaggg taatgcggtc gagtaattta cggcaatgcg cgcaatgagt
    21361 ctggctgtgt ttaaagtctt taagatagcg gcttaatggc cgtcttttca ggtgctgcac
    21421 catcattaag aactcctggt agtcaaagtt aaagtgcggc atttactgac gttataactt
    21481 acccagttta gtgcgtaacc gtttaatggc ctggctgtgt aactgactgc cccgcgattc
    21541 cccgacctcc agcactgcac caatctcttt gagattcagc tcttcctggt aatagagggt
    21601 taataccagt ttttcgcgct ccggcaacgt ttcgatggct tccatcaccc gctggcgcag
    21661 attactgtcc agcagttgtt gtagcgggtt ttcccgctga tgatcatcag taaccagttc
    21721 gatgctatcg ccgtgctctt cgcgccactc atcgtaggag aagagctggc tgttattggt
    21781 gtcgagcaac atttggcgat aatcggcaat atcgatccct aaacgttccg ctacctcagt
    21841 ttccgtggcg ttgcggccaa gttcctgctc cagttgccct attgcctgtg ccacttcacg
    21901 agcgttgcgt cgcacgctgc gcggcaccca gtcacggctg cgaagttcat ccagcatagc
    21961 gccacggata cgctgcactg cgtaagttgt aaatgccgtt ccttgtaggg cgtcatagcg
    22021 ttcgacggca ttaagtaacc caatgccgcc cgcctgtagc agatcgtcaa gttccacgct
    22081 cgtgggcagt cgaacctgca ggcgcaatgc ttcgtgacgc accagcggga cataacgctg
    22141 ccacagcgag tgtttatcca ttacaccttc agcggtatag agtgaattca cgataaacag
    22201 ccctgcgtta tatgagttat cggcatgatt atccgtttct gcagggtttt taatcggacg
    22261 attagtgggt gaaatgaggg gttatttggg gggtaccggt aaattccatg cagaaaaaac
    22321 cccgccggtg gcggggtcag gcaggttaag gccattagcc ttgtaacagt gacagaactt
    22381 gctgcggcac ctggttcgct ttagacagca cggagttgcc tgcctgctga ataatctgcg
    22441 ctttcgacat gttggacact tcggtcgcat agtcggcatc ctgaatacgg gactgcgctt
    22501 cagacaggtt ggtggtggtg ttgttcaggt tggtgatagc ggaatcgaga cggttctgca
    22561 cggcacctag agaagaccgg aatttgtcga ctgatgcaat agctttatcc agaagcgcca
    22621 gcggatcctc agttgaagca ccttcaaatt gctcagtcgc agttgtggtt accttaccat
    22681 tagtatcagt aacatcactt aaattcagat cagttaacgc aagacctgtt gctccattct
    22741 taagaatgac tgccgcatca ccagcagaat agtttttgcc ttgaagtgtg acgaaacctt
    22801 gagctgcgcc gtctttatct gctgaaactt tgatcaactg atctgtttgc ttggcatcgt
    22861 caacaccgtt cgcataatca tcataagtaa catcaatggt gtttaaagtt actttacctg
    22921 aagcggcatc aacagaagca gcatagaagt ctttacctga ctgaacaaca taatcagctg
    22981 ttgccttacc atctttatca agaatgttgt gcagcgacag gcttgacgta tcggatacac
    23041 ccaatttagc tgcaattgct gcagactcag tagtagtacc aaagttaaca tcaacaggtt
    23101 tatcgccagc gtcttgcggg atagatgtga tagcattgct gacattaaga gagtttttag
    23161 aaactgagaa gccattcagc cccaaagttg aagaatcaat tttcttcaga tcgatggaga
    23221 tagtttgacc atcattcgca ccaacctgaa ttttcattga accatctttg gaaagaacat
    23281 taacgccgtt gaactgagtc tgaccggata cgcggtcaat ttcatcgaga cgggatttga
    23341 tttcgtcctg gattgaagac aggtcagaat cagagttagt accggtagtg gcctgaacgg
    23401 tcagttcacg aacacgctgc aagttgttgt tgatttcaga cagtgcgcct tcagtggtct
    23461 gcgccagaga aataccgtcg ttggcgttac gtgcggcctg agtcagacct ttgatgttag
    23521 aagtgaagcg gttagcaatc gcttggcccg cagcgtcatc tttagcgctg ttaatgcgca
    23581 gaccagaaga gaggcgctcg atagaagtcg acagcgcaga ctggttcttg ttgatattat
    23641 tttgagtgat cagcgagagg ctgttggtat taatgacttg tgccatgatt cattttcctg
    23701 ttttcaagtc tacggtttca gtattgggct tccacccttc ggcttcgtcg ccgtcaaccc
    23761 tgttatcgtc tggtcactca caacctttag aatttttttc tagcggtcga aatactcccc
    23821 ttcctcgttg ctgttcgtgt cattaagcca aaaaataaat cgtaaacttt gccccgctta
    23881 cgccgataac cccggtattc gttctatacg tcgcatgatt aaggaattta tatggctagt
    23941 atttcaacgc ttggggttgg gtcggggctg gacttaagca gcatccttga cagtctggag
    24001 gcggcagaaa aatctaccct gacgccgata tcaaaacagc aaagctctta caccgcgaaa
    24061 ctgagcgcct acgggacgct gaaaagtgca ctagagagtt tccagacagc caacacagca
    24121 ctgaataaag ccgatttatt taccgccact tcaaccacca gcagttcatc ggctttcagc
    24181 gccaccacca cgggcagcgc gattgccggg aaatacacca ttagcgttag ccaactggcg
    24241 caggcacaaa cgctgaccac aaaaattagt caaaaagaca gtaaagctgc catcgccacc
    24301 tgcgatagcg tactgaccat ccagcaaggc ggcggtaaag atccggtcac gattgatatc
    24361 agcgcggcta actcgtcact ttccggcatt cgcgatgcca ttaacaacgc caaagctggt
    24421 gtcagcgcca gtatcatcaa tgtgggaaac ggcgaatatc gtctgtccat caccgctaac
    24481 gatacgggca acgataacgc tatgaaactt agcgtcagcg gcgacagcgc cctggaaagt
    24541 tttatgggtt acaacggaac gtcgggtgat agcagcaatg gcatgattga aagcgtcacc
    24601 gcgcaaaacg ccaaactgac agtcaataac gttgagattg aaaacagcag taacactatc
    24661 agcgacgcgc tggaagatat cacactcaac ctgaatgacg tcaccaccgg taatcagaca
    24721 ctgaccatca gtaaagacac atccaaagct gaaaatgtag tcaaagcatg ggtggatgcc
    24781 tataacacat tgcaggatac attcagtagc ttaaccaaat acaccgctgt cgatgccgga
    24841 gcagaaagtc aggactccag taatggagca ttacttggtg actccacact gcgtaccatc
    24901 cagactcagc ttaaaacctt gctcgccaat actcacagca gttcaaacta taaaacgctg
    24961 gcacagattg gcattaccag cgatgccagt accggcaagc tggaaatcgc gaccgacaaa
    25021 ctgcaaacgg cactaaaaaa agatgccgca ggcatcggtg aaatgtttat aggtgacggt
    25081 aaaagtacag gggtaactac cggcattagt agcaacctga ccagttggct ctcttccacc
    25141 gggataatcc aggccgcaaa agatggcgtc agcaaaacgc tgaataattt gaccgaccag
    25201 tacaacgcag ccagtgagcg tatcgatacc ttaatgacgc gctataaagc ccagttcacc
    25261 caattagatg tgttaatgaa ctcgcttaac tccaccagta gctatctgac acagcagttc
    25321 gacacctcaa atagcaactc gaaataatct tcaggagaaa ctatgtacgc ggcaaaaggc
    25381 acccaggcct atgcacaaat tggcgtcgaa agcgccgtaa tgagcgccag ccagcaacag
    25441 ctggtcacca tgctatttga tggagtgctg agcgcactgg ttagagcgcg cctgtttatg
    25501 caggacaaca atcagcaagg caaaggcgtc tctttgtcaa aagcgatcaa catcattgag
    25561 aacgggctgc gggtgagtct tgatgaagag agcaaagacg aactaaccca aaacttgatt
    25621 gctctttata gctatatggt caggcgcttg ctgcaagcca atttacgcaa cgatgtctcc
    25681 gcagtcgaag aagtggaagc attaatgcgc aatattgccg atgcctggaa agagtcttta
    25741 ctcacccctt ctttgattca ggacccagtc tgatgaacaa tgcaccgcat ttgtatttcg
    25801 cctggcaaca actcgtcgaa aaaagccagc tcatgttacg cctggcgaca gaagaacaat
    25861 gggacgaact catcgccagc gaaatggcgt atgtgaatgc ggtgcaggag atcgcacacc
    25921 tgactgaaga ggttgccccg tccaccacga tgcaggagca gctccgcccg atgctgcgcc
    25981 tgattctcga caacgaaagc aaggtaaagc agttattaca gattcggatg gatgaactgg
    26041 cgaaactggt cggtcagtca tcggtgcaaa aatcggtgtt aagtgcctat ggcgatcagg
    26101 gtggctttgt gctggctccg caggataacc tcttttgaat cttaaagagc caatggctcg
    26161 cgaataatct gattacggct acgcttctaa tgttcccctt gaatggagtc gaagaatgcg
    26221 taatcccacg ctgttacaat gttttcactg gtattacccg gaaggcggta agctctggcc
    26281 tgaactggcc gagcgcgccg acggttttaa tgatattggt atcaatatgg tctggttgcc
    26341 gcccgcctat aaaggcgcat cgggcgggta ttcggtcggc tacgactcct atgatttatt
    26401 tgatttaggc gagtttgatc agaaaggcag catccctact aaatatggcg ataaagcaca
    26461 actgctggcc gccattgacg ccctgaaacg taatgacatt gcggtgctgt tggacgtggt
    26521 agtcaaccac aaaatgggcg cggatgaaaa agaagctatt cgcgtgcagc gtgtaaatgc
    26581 tgatgaccgt acgcaaattg acgaagaaat cattgagtgt gaaggctgga cgcgttacac
    26641 cttccccgcc cgtgccgggc aatactcgca gtttatctgg gatttcaaat gttttagcgg
    26701 tatcgaccat atcgaaaacc ctgatgaaga tggcatattt aaaattgtta acgactacac
    26761 cggcgaaggc tggaacgatc aggttgatga tgaattaggt aatttcgatt atctgatggg
    26821 cgagaatatc gattttcgca atcatgccgt gacggaagag attaaatact gggcgcgctg
    26881 ggtgatggaa caaacgcaat gcgacggttt tcgtcttgat gcggtcaaac atattccggc
    26941 gtggttttat aaagagtgga tcgaacacgt acaggaagtt gcgccaaagc cgctgtttat
    27001 tgtggcagag tactggtcgc atgaagtcga taaactgcaa acgtatattg atcaggtgga
    27061 aggcaaaacc atgctgttcg atgcgccgct gcagatgaaa ttccatgaag catcgcgcat
    27121 ggggcgcgac tacgacatga cgcagatttt cacgggtaca ttagtggaag ccgatccttt
    27181 ccacgccgtg acgctcgttg ccaatcacga cacccaacca ttgcaagccc tcgaagcacc
    27241 ggtcgaaccg tggtttaaac cgctggcgta tgctctaatt ttgttgcggg aaaatggcgt
    27301 tccttcggtg ttctatccgg acctctacgg tgcgcattac gaagatgtcg gtggtgacgg
    27361 gcaaacctat ccgatagata tgccaataat cgaacagctt gatgagttaa ttctcgcccg
    27421 tcagcgtttc gcccacggtg tacagacgtt atttttcgac catccgaact gcattgcctt
    27481 tagccgcagt ggcactgacg aatatcccgg ctgcgtggtg gtcatgtcga acggggatga
    27541 tggcgaaaaa accattcatc tgggagagaa ttacggcaat aaaacctggc gtgattttct
    27601 ggggaatcgg caagagagcg tagtgaccga cgaaaacggc gaagcgacct tcttttgcaa
    27661 cggcggcagc gtcagcgtgt gggttatcga agaggtgatt taaattcatc cccggcggca
    27721 agccggggag atttcattac ggcagtggcg tcggtaacgg cgctttatcc agcgcggcgg
    27781 cacactctgc cgtcgggcga tccacacgct ccatcgtcat accatcgtac tcaatggtgt
    27841 taccgtcacg ctcaacctcg tacagctcac gttttacggt aacgttagtg agatcatcag
    27901 acatcaatgt caacttgccc ggcacggcaa tcacccgctg ccactgacgg caatccaggg
    27961 tatcgccctc tttagtgaca atcaggctgc ctatcgcttc cggactgacc agcgcacgtt
    28021 gcggcccttt ggtctgccag tagcccgcca gccagtccgg cgcgggggtt ttcacgacat
    28081 tgttatagtt ttcgacttcg gcacagcccg ccagcaacat gagcgcgctt gcaattgcga
    28141 gttttttcat cattcgtcct gcatgagaag aaaagaggat tgtggcatta aagccctgat
    28201 gtcgccagcc tttccaggtt cagagagaaa tttgatctaa ctattatttt tgtgaattat
    28261 taccccgaaa ataaatttgc atgatgtatg attcgcgatt cttgtttcaa accttctgag
    28321 ttcagaggct acattcatgt catggcagca attcaaacac gcctggttga ttaaattctg
    28381 ggcacccatc cctgcggtca tcgcggcggg tattctctcc acttactatt ttggcattac
    28441 tggcaccttt tgggctgtca cgggtgaatt tacccgttgg ggcggtcagc tcctgcagct
    28501 gtttggcgtc catgctgaag agtggggtta ttttaaaatt atccatctgg aaggatcgcc
    28561 attaacccgc atcgacggga tgatgatcct cggcatgttt ggcggctgct ttgccgcagc
    28621 gctgtgggcc aacaatgtca aactgcgcat gccgcgcagc cgtatccgca ttatgcaggc
    28681 cattattggc ggcattatcg ccggttttgg cgcgcgtctg gcaatgggct gtaacctggc
    28741 ggcgttcttt accggcattc ctcagttctc gctgcatgcc tggttctttg ccatcgccac
    28801 tgccattggt tcatggtttg gcgcgcgctt taccctgctg cccatcttcc gtattcccgt
    28861 gaaaatgcag aaagtttctg ccgcctcacc gctgacgcaa aaaccggatc aggcgcggcg
    28921 tcgttttcgt ctcgggatgc tggtcttttt cggcatgctg ggctgggcgc tgctcacggc
    28981 gatgaaccaa cccaaactgg ggctggcaat gctgtttggc gtcggctttg gtttactgat
    29041 tgaacgtgcg caaatctgct ttacttcagc gttccgcgat atgtggatca ccggacgtac
    29101 ccatatggcg aaagcaatca ttattggtat ggcggtgagt gccatcggga tcttcagtta
    29161 cgtacagtta ggcgttgaac ccaaaatcat gtgggcggga ccaaacgcgg taattggtgg
    29221 tttactgttt ggttttggca tcgtgctggc aggcggctgc gaaaccggct ggatgtaccg
    29281 cgcggtagaa ggccaggtgc actactggtg ggtcggtctg ggcaacgtga tcggctcaac
    29341 gattctggcg tattactggg atgatttcgc tccggcgctg gccaccgact gggacaaaat
    29401 caacctgctg aaaacctttg gcccgatggg tggcctgctg gtgacatatt tgctgttgtt
    29461 tactgcgctg atgttgatta tcggctggga aaaacgcttc ttccgccgtg cggcaccgca
    29521 gactgctaag gagatcgcat gaaaaatatc gttcctgatt accgccttga tatggtgggt
    29581 gaaccctgcc cttatccggc agtcgcaact cttgaggcga tgccgcagtt aaaaaaaggg
    29641 gaaatcctgg aagtggtgag cgactgtcca cagtcgatca ataatattcc actggatgcg
    29701 cgtaatcacg gctatacggt gctggatatt cagcaagacg ggccgaccat tcgttattta
    29761 attcagaagt aatccctcat tcccgctgtg gcccacagcg ggaacatcgt tgcctctgac
    29821 acatagattt tacccgcccc ctctcctatt cttttcagta tgatctcagg aggtttctat
    29881 gtgtggacgc tttgcccaat cccaaacgcg tgaagattac cttgcgcttc tcgcggaaga
    29941 tattgaacgc gatattccct acgatcccga acccattggc agatacaacg tcgcgccggg
    30001 aaccaaagtt cttctgctca gtgaacgtga tgaacacctt catctggatc cggttttctg
    30061 gggatatgct cccggatggt gggataaacc gccgctgatt aacgcccgcg tagaaactgc
    30121 ggccaccagt cgtatgttta aaccgctctg gcaacatggt cgggcaatct gttttgccga
    30181 tggctggttt gagtggaaaa aagaaggcga caaaaaacag ccttatttta tctatcgcgc
    30241 tgatggacaa cctgttttta tggccgcgat aggtagcaca ccgtttgagc gcggtgacga
    30301 agccgaagga tttttaattg tcactgcagc agcagaccag ggtctggtag atattcatga
    30361 ccgccgcccg ctggttctgt cgccagaagc tgcgcgggaa tggatgcggc aagacattgg
    30421 cggtaaagaa gcctcagaaa tcgcggccag tggctgtgtg ccagcaaacc agttctcctg
    30481 gcacccggta tcgcgcgcgg tgggtaatat caaaaaccag ggagcggagt taattcaacc
    30541 ggtttgataa gccaggagac tattgatcaa taatactgcg tcataagaaa tctctattag
    30601 acaaagtaga cgctccttcc acatgcacca caagaacagt gacacaatta cgcctgatta
    30661 tttccctagc ccggaatatg aataaaaaca tcaacaaaga ttaaattatt caaacgttac
    30721 tgttgcgtct gcggtcgtaa caacatccga cgtggtgatg tgtatcaaat gaggcctctg
    30781 atgatcagag ggtttatcgc gtatacggat cgtggatggc agaaaacttt caggatcagg
    30841 tatccatttt aaaccagaaa tagggtgagt ttgccccatc catgccccac gcgggcagat
    30901 gggatgtaat taacaacagg ttacaaaacc tacacctgca tggacatcac ttcctgatac
    30961 gccgccacca gcttattacg cacctgaatc cccatttgca tagaaactga ggctttttgc
    31021 atatcggtca tcacatcgtt taacgccacg ccgggttcac cgagggtgaa tttttctgcc
    31081 tgcgtgcggg cagctgtttg tgtatcgcta atgcgatcga gcgcggcgtg cagctgcccg
    31141 gcaaaactaa tggtcggttg cggcagtgaa tcctgcgcac gcgcactcat cgccgtagcc
    31201 tgtaactggc tgataacccc ttcaatcccc tgtatcgctg acattttcgt ctcctggata
    31261 atttctggta gcaaagccta ccagtaagtc aataagacaa aggcgctaaa tagcaacaaa
    31321 aaaacgggtt tattggcgga tagaaaaaaa cgaaagcaca aataatggca gcgtcaattt
    31381 ttcgagtttg ctgacccggg agtgagtctt gttccacttt gccaataacg ccgtccataa
    31441 tcagccacga ggtgcgcgat gaatgcgact gcagcccaga caaaatctct tgagtggctt
    31501 aatcgcctgc gtgcgaatcc gaaaattcca ttgattgttg ccggttccgc ggcagtggcg
    31561 gtcatggtcg cactgatcct gtgggcgaaa gcccccgact accgcacatt attcagcaat
    31621 ctttccgatc aggatggtgg cgcaattgtc agccaactga cgcaaatgaa tattccttac
    31681 cgcttcagcg aagccagcgg cgctattgaa gttccggcag ataaagttca cgagctgcgt
    31741 ctgcgcctgg cgcaacaagg tttgcccaaa ggcggcgcgg tcggtttcga actactcgat
    31801 caggaaaagt ttggtatcag ccagttcagc gaacaggtga attatcagcg ggcgctggaa
    31861 ggcgagcttt ctcgtaccat cgaaactatc ggcccggtaa aaggggcgcg cgtacatctg
    31921 gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat ccccttctgc atcggtgacg
    31981 gtaaatctgt tacccggccg cgcactcgat gaagggcaaa ttagcgccat tgtacatctg
    32041 gtttccagcg cggttgctgg tctgccgccg gggaacgtca cgctggtgga tcagggtgga
    32101 catctgttaa cccagtccaa taccagcggg cgcgatctta atgacgctca gttgaaatat
    32161 gccagcgatg tcgaaggccg gattcagcgg cgtattgaag cgatcctgtc gccaattgtt
    32221 ggtaacggta atattcacgc ccaggtcacg gcgcaactgg acttcgccag taaagaacaa
    32281 acggaagaac agtatcgccc taacggtgat gaatctcatg cggcgcttcg ttcacgccag
    32341 cttaatgaga gcgagcaaag cggttccggt tatccgggcg gcgtaccggg ggcgttgtcg
    32401 aatcaaccgg cacctgcgaa taacgcgcca atcagcacgc ctccggcaaa tcaaaataac
    32461 cgccagcagc aggcgagcac caccagcaat agcgggccgc gtagcacaca gcggaatgaa
    32521 accagtaact acgaagtcga tcgcaccatt cgtcatacca aaatgaacgt gggcgatgtg
    32581 caacgtctgt cagtcgcggt agtggtgaat tacaaaacct tgccagacgg taaaccattg
    32641 cctctcagca acgaacagat gaagcaaatt gaagatctga cccgcgaggc gatgggcttt
    32701 tctgaaaaac gcggtgactc gctcaatgtc gttaactcgc cgttcaatag cagtgacgaa
    32761 agcggcggag aactgccgtt ctggcaacag caagcgttta tcgatcagtt gctggctgcc
    32821 ggtcgctggt tgctggtact gctggtggcg tggctgctgt ggcggaaagc ggtacgcccg
    32881 cagttaacac gtcgcgccga ggcgatgaaa gctgtacagc aacaggcgca ggcccgtgag
    32941 gaagaggaag atgcggtgga agtccgcctg agcaaagacg aacaactcca acaacgacgc
    33001 gctaaccaac gtctgggggc agaagtcatg agccagcgta tccgtgaaat gtctgataac
    33061 gatccgcgcg tggtggcgct ggtcattcgc cagtggataa ataacgatca tgagtaacct
    33121 gacaggcacc gataaaagcg tcatcctgct gatgaccatt ggcgaagacc gggcggcaga
    33181 ggtgttcaag cacctctccc agcgcgaagt gcaaaccctg agcgctgcaa tggcgaacgt
    33241 cacgcagatc tccaacaagc agctaaccga tgtgctggcg gagtttgagc aagaagctga
    33301 acagtttgcc gcactgaata tcaacgccaa cgattatctg cgttcggtat tggtcaaagc
    33361 tctgggtgag gaacgtgccg ccagcctgct ggaagatatt ctcgaaactc gcgataccgc
    33421 cagcggtatt gaaacgctca actttatgga gccgcagagt gccgccgatc tgattcgcga
    33481 tgagcatccg caaattatcg ccaccattct ggtgcatctg aagcgcgccc aggccgccga
    33541 tattctggcg ttgttcgatg aacgtctgcg ccacgacgtg atgttgcgta tcgccacctt
    33601 tggcggcgtg cagccagccg cgctggcgga actgaccgaa gtactgaatg gcttgctcga
    33661 cggtcagaat ctcaagcgca gcaaaatggg cggcgtgaga acggcagccg aaattatcaa
    33721 cctgatgaaa actcagcagg aagaagccgt tattaccgcc gtgcgcgaat tcgacggcga
    33781 gctggcgcaa aaaattatcg acgagatgtt cctgttcgaa aatctggtgg atgtcgacga
    33841 tcgcagcatt cagcgtctgt tgcaggaagt ggattccgaa tcgctgttga tcgcgctgaa
    33901 aggggccgag cagccactgc gcgagaaatt cctgcgcaat atgtcgcagc gtgccgccga
    33961 tattctgcgc gacgatctcg ccaaccgtgg tccggtgcgt ctgtcgcagg tggaaaacga
    34021 acagaaagcg attctgctga ttgtgcgccg ccttgccgaa actggcgaga tggtaattgg
    34081 cagcggcgag gatacctatg tctgataatc tgccgtggaa aacctggacg ccggacgatc
    34141 tcgcgccacc acaggcagag tttgtgccca tggtcgagcc ggaagaaacc atcattgaag
    34201 aggccgaacc cagccttgag cagcaactgg cgcaactgca aatgcaggcc catgagcaag
    34261 gttatcagga ggggattgcc gaaggtcgcc agcaaggtca tgagcagggc tatcaggaag
    34321 gactggcccg ggggctggag caaggtctgg cagaggcgaa gtctcaacaa gcgccaattc
    34381 atgcccggat gcagcaactg gtcagcgaat ttcaaactac ccttgatgcg cttgatagtg
    34441 tgattgcttc gcgcctgatg cagatggcgc tggaggcggc acgtcaggtg atcggtcaga
    34501 cgccaacagt ggataactcg gcactgatca aacagatcca acagttgttg cagcaagaac
    34561 cgttattcag cggtaaacca cagctgcgcg tgcacccgga tgatctgcaa cgtgtggatg
    34621 atatgctcgg cgcaacctta agtttgcatg gctggcgctt gcggggcgat cccacactcc
    34681 atcctggcgg ctgtaaagtc tccgccgatg aaggcgatct cgacgccagt gtcgccactc
    34741 gctggcaaga actctgccgt ctggcagcac caggagtggt gtaatgagca cgcgcctgac
    34801 tcgctggcta accacgctgg ataactttga agccaaaatg gcgcagttgc ctgcggtacg
    34861 tcgctatggg cgattaaccc gcgctaccgg gctggtgctg gaagccaccg gattacaatt
    34921 gccgctcggc gcaacctgtg tcattgaaca ccagaacggc actgaaacgc acgaagtaga
    34981 aagtgaagtc gttggcttta acggtcaacg gctgttttta atgccgctgg aggaagtcga
    35041 aggtgtcctg cccggcgcgc gtgtttatgc caaaaacatt tcggcagaag ggctgcaaag
    35101 cggcaagcag ttaccgctcg gtccggcgtt attaggtcgt gttctggacg gcagcggtaa
    35161 accgctcgat ggcctcccct cccccgatac gacggaaacc ggtgcgctga ttaccccgcc
    35221 atttaacccg ttgcaacgta caccgattga acatgtgctg gacaccgacg tgcgcccaat
    35281 caatgccctg cttaccgttg ggcgtgggca gcgtatggga ctgtttgccg ggtctggcgt
    35341 tggtaaaagt gtgctgctgg ggatgatggc ccgttacacc cgcgccgatg tcattgtcgt
    35401 gggtttgatt ggtgaacgtg ggcgcgaagt aaaagatttt attgagaata tcctcggtgc
    35461 cgagggacgt gcacgctccg tggtgattgc cgctccggcg gatgtctctc cgcttctgcg
    35521 aatgcaaggt gccgcctatg ccacgcgaat tgccgaagat tttcgcgatc gtggtcagca
    35581 tgtgttgctg attatggact ccctcacccg ctacgcgatg gcccagcgtg aaattgcgct
    35641 ggcgattggc gaaccccccg ccaccaaagg ttatccaccg tcggtgtttg ccaaattacc
    35701 ggcactggtc gagcgtgccg gaaatggcat tagcggcggc ggctcgatta ccgcgtttta
    35761 taccgtgctc actgagggcg atgaccagca ggaccccatt gccgactccg cgcgggcaat
    35821 cctcgacggt cacattgtgc tctctcgccg actggcggaa gccgggcact atccggctat
    35881 cgatattgaa gcgtcgatca gccgcgcaat gacggcgttg atcagtgagc aacattacgc
    35941 gcgagtgcgc accttcaaac agctgttatc gagttttcag cgtaaccgcg acctggttag
    36001 cgtcggcgcg tatgccaaag gcagcgatcc gatgctcgac aaagccatcg ccctgtggcc
    36061 gcagctggag ggctatttgc aacaaggcat ttttgaacgc gcggactggg aagcgtctct
    36121 ccaggggctg gagcgtattt tcccgacagt gtcataaccc aggagataac ggcagatggc
    36181 agaacatggc gcgctggcga ccctgaaaga tctggcagaa aaagaggtag aggatgccgc
    36241 gcgcctgctg ggtgaaatgc gtcgcggatg tcagcaggcg gaagaacagc tcaaaatgct
    36301 gatcgattat cagaatgaat atcgcaataa cctcaacagc gatatgagtg ccgggatgac
    36361 cagcaaccgc tggatcaact atcagcagtt tatccagacg ttggaaaaag ccattactca
    36421 gcatcgccag caacttaatc agtggacgca gaaagttgac attgccctga acagctggcg
    36481 agaaaagaaa caacgtttgc aggcctggca gacactgcag gaacggcaat ccacggcggc
    36541 actgcttgca gaaaaccgcc tcgatcagaa aaagatggat gagttcgccc agcgcgccgc
    36601 catgaggaaa cctgaatgat tcgcttagcg cccttgatta ccgccgacgt tgacaccacc
    36661 acattgcctg gcggcaaagc cagcgatgct gcacaagatt ttctcgcgtt gttgagcgaa
    36721 gcattagcag gcgagacaac taccgacaaa tcggcccccc agttgctggt ggcaacagat
    36781 aagcccacga caaaaggcga gccgctgatc agcgatattc ttgccgacgc gcaacaagcg
    36841 gatttactga tccccgtgga tgaaacactg cctgtcatca acgacgaaca atccacatca
    36901 acaccgttaa ccaccgctca gacgatgacg ttggctgcgg tggctgacaa aaatacgaca
    36961 aaagacgaaa aagcggatga tctgaatgaa gacgtcaccg caagcctgag cgcccttttt
    37021 gcgatgttgc cgggttttga caatacgccc aaagtgactg atgtgccgtc aaccgtgtta
    37081 ccggcagaga aaccaacgct cttcacaaaa ctgacttctg cgcaactcac aacagcacag
    37141 cctgatgatg cccccggcac gccagctcag ccattaacac cgctggtagc agaagcccag
    37201 agtaaagcgg aagtcatcag cacaccgtca ccggtgaccg ctgccgccag cccgctaatt
    37261 actccacacc agacacagcc actgcccacc gtcgccgcgc ctgttttgag tgcaccgctg
    37321 ggttctcacg aatggcaaca atcattaagc cagcatattt cgctgttcac ccgccagggg
    37381 caacaaagtg cagagttgcg tctgcacccg caggatttag gtgaagtgca aatctccctc
    37441 aaagtggatg ataaccaggc acaaatccag atggtttcac cgcatcagca tgtacgcgcc
    37501 gcgctggaag cagcgcttcc ggtactgcgt acgcagctgg ccgaaagtgg cattcagtta
    37561 gggcaaagca acatcagtgg cgaaagcttt agtggtcagc agcaggccgc ttcccaacaa
    37621 cagcaaagcc aacgcacagt aaaccatgaa cctctggcgg gggaagacga cgatacgctt
    37681 ctggttcccg tctctataca agggcgcgta acaggcaaca gcggcgttga tattttcgcc
    37741 taacgtcaga ggtagcaccg taatccgcgt cttttccccg ctttgttgcg ctcaagacgc
    37801 aggataatta gccgataagc agtagcgaca caggaagacc gcaacacatg actgattacg
    37861 cgataagcaa gaaaagcaag cgatcgcttt ggatcccgat tctggtattc attaccctcg
    37921 cggcctgtgc cagcgcaggt tacagctact ggcattcgca tcaggttgcc gctgacgaca
    37981 aagcgcagca acgcgtcgta ccctccccgg tcttctacgc gctggatacc ttcacggtca
    38041 atttgggcga tgcggatcgc gtgctttata tcggcataac cctgcgcctg aaagatgaag
    38101 ctacccgctc gcggctgagt gagtatttgc cggaagtccg tagccgtttg ctgttactgt
    38161 tttcgcgtca ggatgctgcc gtactggcga cagaagaagg caagaaaaac ctgattgccg
    38221 agattaaaac cacgctttcc cccccgcttg ttgccgggca accgaaacag gatgtcaccg
    38281 acgtgctgta taccgctttt attctgcgat aacgacatgg gcgatagtat tctttctcaa
    38341 gctgaaattg atgcgctgtt gaatggtgac agcgaagtca aagacgaacc gacagccagt
    38401 gttagcggcg aaagtgacat tcgtccgtac gatccgaata cccaacgacg ggttgtgcgc
    38461 gaacgtttgc aggcgctgga aatcattaat gagcgctttg cccgccattt tcgtatgggg
    38521 ctgttcaacc tgctgcgtcg tagcccggat ataaccgtcg gggccatccg cattcagccg
    38581 taccatgaat ttgcccgcaa cctgccggtg ccgaccaacc tgaaccttat ccatctgaaa
    38641 ccgctgcgcg gcacagggct ggtggtgttt tcaccgagtc tggtgtttat cgccgtggat
    38701 aacctgtttg gcggcgatgg acgcttcccg accaaagtgg aaggtcgcga gtttacccat
    38761 accgaacagc gcgtcatcaa ccgcatgtta aagctggcgc ttgaaggcta tagcgacgcc
    38821 tggaaggcga ttaatccgct ggaagttgag tacgtgcgtt cggaaatgca ggtgaaattt
    38881 accaatatca ccacctcgcc gaacgacatt gtggttaaca cgccgttcca tgtggagatt
    38941 ggcaacctga ccggcgaatt taatatctgc ctgcctttca gcatgatcga gccgctacgg
    39001 gaattgttgg ttaacccgcc gctggaaaac tcgcgtaatg aagatcagaa ctggcgcgat
    39061 aacctggtgc gccaggtgca gcattcacag ctggagctgg tcgccaactt tgccgatatc
    39121 tcgctacgcc tgtcgcagat tttaaaactg aaacccggtg acgtcctgcc gatagaaaaa
    39181 cccgaccgca tcatcgccca tgttgacggc gtcccggtgc tgaccagtca gtacggcact
    39241 ctcaacggtc agtatgcgtt acggatagaa catttgatta acccgatttt aaattctctg
    39301 aacgaggaac agcccaaatg agtgacatga ataatccggc cgatgacaac aacggcgcaa
    39361 tggacgatct gtgggctgaa gcgttgagcg aacaaaaatc aaccagtagc aaaagcgctg
    39421 ccgacgcggt gttccagcaa tttggcggtg gtgatgtcag cggaacgttg caggatatcg
    39481 acctgattat ggatattccg gtcaagctga ccgtcgagct gggccgcaca cggatgacca
    39541 tcaaagagct gttgcgtctg acgcaagggt cagtcgtggc gctggacggt ctggcgggcg
    39601 aaccgctgga tattctgatc aacggttatt taatcgccca gggcgaagtg gtggtcgttg
    39661 ccgataaata tggcgtgcgg atcaccgata tcattactcc gtctgagcga atgcgccgcc
    39721 tgagccgtta gtgatgaata accacgctac tgtgcaatct tccgcgccgg tttctgctgc
    39781 gccactgctg caggtgagcg gcgcactcat cgccattatt gccctgatcc tcgctgctgc
    39841 ctggctggta aaacggttgg gatttgcccc gaaactaact ggcgttaacg gtctgaaaat
    39901 tagcgccagt gcttcgctgg gcgcgcgtga acgggttgtg gtggtcgatg tggaagatgc
    39961 acggctggtg ctcggcgtta ccgcagggca aatcaatctg ctgcataaac ttcccccttc
    40021 agcaccaacg gaagagatac cgcagaccga ttttcagtcg gtcatgaaaa acttgcttaa
    40081 gcgtagcggg agatcctgat gcgtcgtttg ttgtctgtcg cacctgtcct tctctggctg
    40141 gttacgcccc tcgccttcgc gcaactgccg ggtatcacca gccagccgct gcctggcggt
    40201 ggacaaagct ggtcgctccc ggtgcagacg ctggtgttca tcacctcgtt gacgttcatt
    40261 ccagcgattt tactgatgat gaccagtttc acccgcatca ttattgtttt tggtttattg
    40321 cgtaacgcgc tgggaacacc ctcagcgcca cctaaccagg tattgctggg gctggcgctg
    40381 tttttgacct tttttattat gtcgccggtg atcgataaaa tttatgtcaa tgcgtaccag
    40441 ccattcagcg aagagaaaat atcaatgcag gaggcgctgg aaaaaggggc gcagccgctg
    40501 cgtgagttta tgctgcgtca gacccgtgag gcggatttag ggttgtttgc cagactggcg
    40561 aatactggcc cgttgcaggg acctgaagcc gtgccgatgc gcattttgct cccggcctac
    40621 gtgaccagcg agttgaaaac cgcatttcag ataggcttca cgattttcat cccttttttg
    40681 attatcgacc tggtgattgc cagcgtgttg atggcattgg ggatgatgat ggtccccccc
    40741 gccaccattg ctctgccctt taaactgatg ctgtttgtac tggtggatgg ctggcaattg
    40801 ctggtcggtt cgctggcgca gagcttttac agctagagag gcaaaatgac acctgaatcg
    40861 gtcatgatga tggggactga agcgatgaaa gtcgcgctgg cactggctgc cccgctgttg
    40921 ctggtggcgc tggtcacggg ccttatcatc agtattttgc aggccgccac gcagatcaac
    40981 gaaatgacgc tgtcgtttat tccaaaaatc atcgccgtat ttattgccat tattattgcc
    41041 ggaccgtgga tgctcaatct gttgctggat tacgtccgca ccttgttcac taacctgccg
    41101 tatatcatcg ggtagcccgt acaatgatgc aggtgacaag cgatcaatgg ctttcctggc
    41161 taagtctgta cttctggccg ttactgcgcg tgctggcgct catttctacc gcgccaattc
    41221 tgagcgaacg cagcgtgccg aaacgggtaa aactgggtct ggcaatgatg atcacgttcg
    41281 ctattgcccc atcattacct gccaacgatg ttcctgtttt ttcgttcttt gctctgtggc
    41341 tggccgtgca gcagatcctg atcggcattg cgcttggttt taccatgcaa tttgcctttg
    41401 ccgctgtgcg aaccgctggc gaaattatcg gtctgcaaat ggggctgtca tttgcgacgt
    41461 ttgtcgatcc ggccagccat ctcaatatgc ctgttttagc gcgtatcatg gatatgctgg
    41521 cgttactact gttcctgaca tttaacggtc atttatggtt gatttcactg ctggtcgata
    41581 cctttcacac cctgccaatt ggtggcgaac cgttgaacag caatgcgttt ctggcactca
    41641 ccaaagcagg gagtttgatt ttccttaacg ggctgatgct ggcgttaccg ctcattactc
    41701 tgctgctgac actgaatctg gcattaggtt tacttaatcg tatggccccg caattatcca
    41761 tttttgttat tggatttcca ttaactctga ctgtcggtat ctctttaatg gcggcattaa
    41821 tgccgttaat tgcacctttt tgcgaacatt tattcagtga gatgtttaat ttgctggctg
    41881 atattattag tgaattgcca ttaatataat tccgtaacgt ttatcatgtc atcctaagga
    41941 ttatccgaaa aataatacct acgaacatct tccaggatac tcctgcggcg aaatatttgt
    42001 tttaagctca ctcacatatc gcaacattta ctttacttta agacaattcc aggcaaatta
    42061 tacaacactt tacgggatag taagtccgcc tgaaaaatcg cgagagtggc gcattaggtg
    42121 acccatgttg ttccgtttag tcatgatgaa atattcaggt aaggggaatt atcgttacgc
    42181 attgagtgag ggtatgccat gtcaacgatt attatggatt tatgtagtta cacccgacta
    42241 ggtttaaccg ggtatctgtt gagtagaggg gttaaaaaaa gagaaatcaa cgacattgaa
    42301 accgttgatg accttgccat agcttgtgat tcacagcgcc cttcagtggt gtttattaat
    42361 gaggactgtt tcatccatga tgcttctaac agtcagcaca tcaagcacat cattaatcaa
    42421 catcccaata cgttatttat cgtttttatg gcaattgcca atgttcattt tgatgaatat
    42481 ctattggtca gaaaaaattt attgataagt tctaaatcga ttaaaccgga atctctcgac
    42541 gatatccttg gcgatattct gaaaaaagag acaacgataa cctctttttt aaatatgccg
    42601 acgttatcat tgagccgaac cgaatcgagt atgttgcgaa tgtggatggc aggtcaggga
    42661 accattcaaa tctctgacca aatgaatatc aaagccaaga ccgtttcatc gcataaaggt
    42721 aatattaaac gtaagatcaa aacgcataat aaacaggtta tctaccatgt cgtccgactg
    42781 acggataatg tgactaatgg tatttttgtc aacatgcgct aacacattct gactggtggt
    42841 ttcccaccag tcaggctgaa taagattact ctgctttctc cacaaagata ccgtcctgat
    42901 gccctgcttc attaaagaac cagatgccga gcgggtagtc ttccagcgaa accaggtaca
    42961 ttgtgccttc actaaactcc tcaactgcca gtaccacgcc aggacgacgc ggaccgccat
    43021 ccgttttgac tgttacccga tcattcacct tcatcgtttt cctcctgtgg ctttgtgcca
    43081 gtgtagaaca atttcgtttt tctggcagcg ccaggcgcgc gcgagtgctg attttctcga
    43141 cggtctatac ttaagagatg ccaggcggac ttaacgactg gcggcaacaa cagagtaacg
    43201 gttgcgagga aagatgatga aaaccgcaaa agagtacagc gataccgcaa aacgtgaggt
    43261 cagcgtcgat gtcgatgccc tgctggcggc gatcaatgaa attagcgaaa gcgaagttca
    43321 tcgcagccag aacgattctg aacacgttag cgtcgatgga cgtgaatatc atacatggcg
    43381 tgaattggcg gatgccttcg aactggatat tcatgacttc agcgtctctg aagtgaatcg
    43441 ttgaatgcac aataaaaaaa tcccgaccct gagggggtcg ggatgaaact tgcttaagca
    43501 agaagcactt aaaaaattcg ttacaccagg aaatctgatg tgttcatcac cttatccgca
    43561 atttttttcg ctgacaagaa aatattcgca atatgaatga ttaacgctac ttatgaatag
    43621 aaatgtgacg tgcgtcaccc cttggtggct ctggcgtcaa cgggatcgct cccgaaaaaa
    43681 tcatctttga ttatttgtgc tgttttgaga atcatcctgg taaggggaga actgcgcgac
    43741 catgttttca attcaacaac cactactggt ttttagcgat cttgatggca ccctgctgga
    43801 cagtcatagt tatgactggc aaccggctgc cccctggctc agccgtttac gcgaagcgaa
    43861 tgttcccgtc attctctgta gcagtaaaac ttcagcggaa atgctgtact tgcaaaaaac
    43921 gttggggcta caagggttac cactgattgc cgaaaatggc gcagtgatcc agcttgctga
    43981 acaatggcag gatatagacg gttttccacg catcatctca ggtattagcc atggcgaaat
    44041 cagccaggtt ttaaatacgc tacgcgagaa agaacatttt aaattcacga ctttcgatga
    44101 tgtcgacgat gcaaccatcg ccgaatggac gggattaagc cgtagccagg cggcgctgac
    44161 gcagctgcat gaggcgtcgg taacgctaat ctggcgcgac agtgacgagc gtatggcaca
    44221 atttaccgct cgtctgaacg aactgggctt acagtttatg cagggtgcgc gcttctggca
    44281 cgtcctggat gcctctgccg gaaaagatca ggccgccaac tggattatcg cgacctatca
    44341 acaattatca ggcaaacgcc caaccacact tggcctgggc gatgggccaa acgatgcgcc
    44401 cttactggag gtaatggatt acgcggtgat tgtgaaaggg ctaaaccgtg aaggggtgca
    44461 tctgcatgat gaggatccga cccgcgtctg gcgaacgcag cgtgaaggac cggaaggctg
    44521 gcgtgaaggg ctggaccatt ttttctccgc tcgttaagcg ttatcgctcg cgcatacccg
    44581 attacgcccg gcctgtttag cgagataaag ccgacggtca gccagtgact ggagttgttc
    44641 aaaatcataa tcaccggttt cctcgctgct acttaccccc aacgaggcac tgatgcgtat
    44701 cgtcgtactc ttagcaatca acatctcttt ttcatttaac ttcaggcgaa tacgttctgc
    44761 gacttccgca gcctccgtca gactcgcgcc tggcagaatc acacaaaact cctcaccacc
    44821 gacccgcccg gcaacgtcct gcgcacgcaa ggaactgcta attaatccgg cagcatgaga
    44881 aagaacacgg tcgcccgcct gatgaccaaa gcggtcatta atcgctttaa aatggtcaag
    44941 gtcgacctga atgacagaaa aaggatgttg gtgcgtctga cacaatttcg cgagcggacg
    45001 ggctttttca aacagtgcgc cacgattata taaacgggtt aaggtgtcgt gccacgcctg
    45061 ccactgcaac gagctttgca gaacatacat gttgctaacc atccggcgaa tcacatacca
    45121 ggagatgagt aacatggtgg taaagagcgc ccacagcagg gttaatgcaa tgctgatact
    45181 gccgaaatcg ccgcgcacgc cttcgcttag cgtatggacg cgcaccagca caccgtcgaa
    45241 atggtccaga cgttcccagc taacatagcg actgtccata cgaatgccgc cccgcgtgtc
    45301 atgttccatc gcctgcgcca gcaaggccag ttcacgagga tcaaaaatat tccctgttgg
    45361 atggtcagga ttggaagagg tcaaaaatct cagcttactg tcatagagct gatactcacc
    45421 atcgaggttt ttatcgatgg cgtttcttaa aaattgctgc atagtacgta cgggaatact
    45481 catccccagc acgccatacc agtagttatt actgtctacc ggaacactga cggtaacctg
    45541 cggttcagta ttgctggcgt gttccggttg cgaagtgaac cagcgtaccg cgcggtgacg
    45601 attttctcgt tgtgaatggc cgataaacca gggttgggtg acatagccgt aataacgcgt
    45661 tggaacattg cgcgtaaaca aggtcggctg cgtcgaaacg taaaatccgg cacgcgagac
    45721 atacatcgcc tgttcaacca tcgacgagga gttgtgcgcc agtcgcagca agtaaccaac
    45781 ttccagtgca gcggtaattt cattgtcgag gctttcattt tcgcgagaca ggagatttcc
    45841 ctcgctgact aatgcatccg atacgccatt gactggcagg gtgcgtcgtc ggttgagttc
    45901 gattttccag gcgtgctcgt cgcgatgctg ttcgaactcg gttacagcat cacgcagaga
    45961 ggtgaaatcc agtggcgcta cgagagcttc gcgcatgcca ttacgcaaaa agatcagttt
    46021 gtcgacgtta tactgcaaat gcttatcgag cgcgttggct acgttttcca gatgattacg
    46081 ctggctggag atataggcat cttccagcac taccacttca cgccaggtca gcaaggtgga
    46141 aaaaagcaat accacgataa agcagagatt aacgacatga ccaggcccca ggcggcgtgc
    46201 gagttttttc aaccagctct ggttttccat ttttgtctcg tgctgcaccc tgacgactcc
    46261 caacctgctt ttttatgatt ctggcataac ctggcgatag cgtaccgcaa aacagcgata
    46321 attttataat ggtggcatga taagacgcga ctatcattca ttgcaggcat tggcacacca
    46381 ggccggaagt gatccggcct ggtgttaatg ataccctttc aaacttcttc cgcagaacga
    46441 ctcccctccc ccggaacaag ctcggtttca tgaaaagctt cccgcttgac cccgtaaccg
    46501 tcataccaac gacactcaac cataccgctg gagtatccgg tgacaatcat ccgcgggccg
    46561 ccctctttaa ccgtaacttc ctcactaacc ataaagctca tactcgcctc cttttttcga
    46621 gtgaaacttg ttcaccttag ttgaagatgg cgaattttgc ataatagcct gtgcgagtat
    46681 taatgtgcct gaccgcgtat tttcgctacg gctttcactc ccagcaccac gatgccgccg
    46741 atgataaatc caagaatcag atttaaaaca gtcggtaata tcatcgccac cactgcactt
    46801 tgctgcccgg cgaaatgttc aatggcgtga tgcagcggcg caataccatg taccacaatc
    46861 ccgccgccga cgaggaacat cgccagcgtg ccgacaatcg ataacgcttt catcagccag
    46921 ggcgcaatga tcaataaccc tttacctaat gcctgcatca gcgcgctgga tttttccgcc
    46981 agccaatacc ccaggtcatc aatcttaacg ataaccccta ccagaccgta gacgcccacg
    47041 gtcactacca gcgcgatgcc tgaaagcacc agcacctgat taagcaacgg cgcttcggcc
    47101 acaatcccca gcgtgatggc gacgatttcg gcagacaata taaaatcggt acgaatcgcc
    47161 ccttttattt tgtccttttc aaacttcagc ggatcctgcg ccgccagctt ctccagacgc
    47221 tgctggctct gcgccggatc ttctttatgt ttacgcgcct ccagcatatg cagcactttc
    47281 tctactcctt caaagcagag aaacgcgcca ccaatcatca acagaggcgt aatcgcccac
    47341 gggataaacg cactgatgat cagcgccagc ggcaccagaa tcactttatt aatcagcgat
    47401 cctttcgcca cgccccagac cacgggaagt tcccggttgg cccgcacgcc tgaaacttgt
    47461 tgcgcattga gcgataagtc atcccctaat acaccggcgg ttttcttcgc cgccagtttg
    47521 cccatcacgg agatatcgtc cagtagtgtg gcgatatcat cgagcaacgt cagtaaacta
    47581 ctcccggcca aaagcattcc ctctcatttt tattgttgaa gggtgtaagt atgaagcaaa
    47641 aacggcagct ctgatacttg cttcacaggt caacattttt ttaacaatca agtgcaatta
    47701 tatctctcgc cagcagaatg attttcacgt ttactatgaa ccaccttttt atttccaccg
    47761 tgagggatta tgcgtttccg ccagttgtta ccgctttttg gcgcgctgtt tgcgttgtat
    47821 atcatttggg gctcaaccta ttttgtcatt cggattggcg tggaaagctg gcctccgtta
    47881 atgatggcgg gccttcgatt cctggcagcc ggtattttat tgctggcatt tttgctactg
    47941 cgcggacaca agctcccccc gctacgtccg ctgctcaatg ccgcgctgat tggcctgtta
    48001 ttgctggctg tcggtaatgg catggtgacg gttgccgaac atcaaaatgt tccttccggc
    48061 atcgccgccg tagtggttgc aaccgtgccc ctctttaccc tgtgcttcag ccgcctgttt
    48121 ggcattaaaa cgcgcaaact ggaatgggtg ggtattgcca ttgggcttgc cggaatcatc
    48181 atgctcaata gcggtggaaa tttaagcggc aatccgtggg gcgcgattct gattttaatc
    48241 ggctcgatta gctgggcgtt tggctcggtt tatggctcgc gcattacctt acctgtaggg
    48301 atgatggcgg gtgcgattga gatgctggcg gcaggcgtgg tgttaatgat cgcgtcgatg
    48361 attgcaggtg aaaaactgac ggcgctccct tccctttcag gcttccttgc ggtcggctat
    48421 ctggcgctgt ttggttcgat tatcgccatc aacgcttata tgtatttaat ccgtaatgtc
    48481 agtccggctc tcgccaccag ctacgcctac gttaacccgg tggtcgcggt cttgctgggt
    48541 acgggactgg gcggagagac gctgtcgaag attgaatggc tggcgctcgg cgtaattgtc
    48601 ttcgcggtgg tactggtcac gttgggaaaa tatctcttcc cggcaaaacc cgtagttgcg
    48661 ccagttattc aggacgcatc aagcgagtaa atgaatcccc tgcgtgtcga tctgcgcgct
    48721 ggcaccttcg ccgcagatcc actcttccag acgctcggta agcgcttcat ccgtcagctt
    48781 ctcgcgccca cgtaatgcac actcccagac aatcagtacg cgccagccga gttcctgcaa
    48841 acgactgata tcgcggcgat cgcgttcaac atttttacct atcttctcca gccagaactc
    48901 ggttcgagtc gcaggcactt taaacagata gcagtgatga tgatgccaga agcagccatg
    48961 ggtaaatatc acgcagcgat attcatcaac gacaaaatcc gggcgtccgg gcagactggc
    49021 gtcctgaacg cgaaacgcca ggccctgccc ggttaacaga ctggcgaggc gcttctcaat
    49081 cgccgtatca cgcgtggcaa tcgcgcgcat atttttgcta cgagtggcct tatcgtgaac
    49141 gtcggccatg ttgtgcctct tgctgacgca acgccaccgc ctgtttgatt tttggctcaa
    49201 gcagttttgc caccgcggca aagaccggca cgaccaccga gttaccgaac tggcgatagg
    49261 cctgagtgtc cgaaaccgga atgcggaatt tcgcttctcc cggcgcttca aaacccatta
    49321 agcgcgcgca ttcccgaggc gttaaccgac gtggacgatg ttgctgattc agcggatcgt
    49381 caaaatcttt ctcacccgtg gccatatccc agccgcgatc gattaaaatt tccgcgccat
    49441 ctttgtaata acgcgcagac agcgtacgcg tgacgctttg cggattgttc ggataaacca
    49501 ttccataacc gaagccgtta ccgcgcgcct gatgtttttt cgcatatcga tagaggtact
    49561 tccacagcac cggcgtcagg atatatttcg cctcgaccat cgggtccaac agctgcgcca
    49621 gcgtcactcg ctgtgcaggg aaacattcgc tgatatcacg cagagtaaaa tcggctttca
    49681 gattaagatc gcgacgaaaa cccaccagca cgatgcgttc acggtgctgc ggcagaaaat
    49741 gtttaccatc gatgattttc ggatcgtccg gcccgttatc ttctgcatca gccacgtcat
    49801 agcccagttc gtccagcgtc tgcatgatga tgcggaacgt tttaccctgg tcgtgacttt
    49861 tcaggttttt gacgttttcg agcacaaaca tcgccggacg acgcgcgtcg ataatgcgca
    49921 ccacatcaaa aaacagcgtg ccctgggtat cgcaggcaaa accgtgcgcc cgcccgagcg
    49981 agttcttttt cgatacgcca gccagcgaaa atggctgaca agggaaaccg gccagtaaaa
    50041 catcgtgttc aggaatgtgt tgacgaatat gttccgccgc cgcctcatca ctcacgcctt
    50101 ctttatggct gagggtgatg tcgcggatat cttcattaaa atgatgcgtc gccggatcgc
    50161 aataatggtt ggctttataa gtgcgtaccg catgtttgtt ccattcgctg gtaaacacgc
    50221 attgtccgcc aatcgattca aaaccgcgac ggatgccgcc aattccggca aatagatcga
    50281 taaagcgaaa cgcataatgc ggatgatgtg ccggtggttt gggtaacaac gtttgcagat
    50341 gggcgaactc tttctcactt aaacggtgcc atgccgagtc attcgccagc gcacgtttta
    50401 aaattgccgc gctccagtga ttctcaccca caccattaag ctgcgccacc agcgttttaa
    50461 catcataaat ttgcagcagt ttctccagca ttgcctgtgc ggcattcccg gtgctgtatg
    50521 aatcggttac tgatatattt tcctgcatag atttaaccgg ccatctgaga tggccgacag
    50581 gttaacataa ttacagccat ttagggccac gcgcttatcc cgcgggtgaa aacgcatcta
    50641 tcaccttgtg atcgacacct tcattctccc ccgccagttc ggcactgagc ttcgccataa
    50701 actcgactaa aaaatgcgcg ttgtgctgcg ccagctgctt gcctcttgcg gtttgcatgg
    50761 tttgcggtag cttcagcaac ttagtctgga aatgatccag cgcgtagcgt ttatcatcaa
    50821 gcgggcgatg ctgtgcgaac ggatcttcac catcaaacag cgccacgccc aatgcccctg
    50881 aaacggcaaa cacgtgcgcc aggccaatcg cccccagggc ttccagccga tccgcatcct
    50941 ggacgatttt agcttccgtt gttaaggggg cgatttgcgc gctgaaactg tgagcggcaa
    51001 tggcatgaca aacggcctcg attttctccg ccggaaattg ctcgaactct tcacggagca
    51061 ggcgacgcgt ctcttctgcc gccaggattg aagaacgctg cctttgcgga tgatttttcg
    51121 ccaggctcac aatatcgtga caataacagg cggttaaaat caccagcata tcgacatcgt
    51181 catctgccgc cagcttctgt gccgtagccc agacgcggcg aaaatgacac acgtcatggg
    51241 ccgcgtcctg gtgctgatga tggtttttca accagttttc gaactgtgcc tgccagtgtt
    51301 gtaagtccat acgcgctccg ttgccaaaga gtcgccacct tagcagtttt tattcatagc
    51361 gtctgctacg tgcgggacga ggtcgggtgt accaggcaat gctgcctaac aatgccccga
    51421 ccagagccat aaataaaaat ccgaccagtg cccctaacca ctttcccgcg gtagagccta
    51481 aatcgctttc aaatcccgac acgggggcat cggggatccg gctcatcacc cagatgtaac
    51541 gcgccattgc ccagacgaaa tagccacacc atgtatagaa agcccacaaa gccagtttgc
    51601 cgccagggct gcgagagagt ctctcttcca tctttgtcga accgtattcc attttgagat
    51661 aacccggaaa attcagactt agtactatta tgtgataaaa gtcacatttt tccacattgg
    51721 atgagccagt caagactcaa tcattttttg aacaaataga taaattttgc gcaacggcaa
    51781 taattactgg aaaaacagca ataaagattt atttatatcc ttggtaatag ttttattaat
    51841 tcacccaaac aatatattag cactgaatac aattatacct tttttgattt ctgtgtcatg
    51901 ataaccccgt tattagcctt ttatcgtctt gtttatattt
//
LOCUS       DAZKHC020000024        49997 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZKHC020000024 DAZKHC020000000
VERSION     DAZKHC020000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 49997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.024"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(97..384)
                     /gene="yebO"
                     /locus_tag="ACHOSM_RS08340"
                     /old_locus_tag="pgaptmp_001668"
     CDS             complement(97..384)
                     /gene="yebO"
                     /locus_tag="ACHOSM_RS08340"
                     /old_locus_tag="pgaptmp_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HGU4558215.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(459..602)
                     /gene="mgrB"
                     /locus_tag="ACHOSM_RS08345"
                     /old_locus_tag="pgaptmp_001669"
     CDS             complement(459..602)
                     /gene="mgrB"
                     /locus_tag="ACHOSM_RS08345"
                     /old_locus_tag="pgaptmp_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HGU4558216.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            761..1000
                     /gene="yobH"
                     /locus_tag="ACHOSM_RS08350"
                     /old_locus_tag="pgaptmp_001670"
     CDS             761..1000
                     /gene="yobH"
                     /locus_tag="ACHOSM_RS08350"
                     /old_locus_tag="pgaptmp_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HGU4558217.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1143..1934)
                     /gene="kdgR"
                     /locus_tag="ACHOSM_RS08355"
                     /old_locus_tag="pgaptmp_001671"
     CDS             complement(1143..1934)
                     /gene="kdgR"
                     /locus_tag="ACHOSM_RS08355"
                     /old_locus_tag="pgaptmp_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HGU4558218.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2111..3484
                     /gene="yebQ"
                     /locus_tag="ACHOSM_RS08360"
                     /old_locus_tag="pgaptmp_001672"
     CDS             2111..3484
                     /gene="yebQ"
                     /locus_tag="ACHOSM_RS08360"
                     /old_locus_tag="pgaptmp_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4558219.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3530..4411)
                     /gene="htpX"
                     /locus_tag="ACHOSM_RS08365"
                     /old_locus_tag="pgaptmp_001673"
     CDS             complement(3530..4411)
                     /gene="htpX"
                     /locus_tag="ACHOSM_RS08365"
                     /old_locus_tag="pgaptmp_001673"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HGU4558220.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4603..6651)
                     /gene="prc"
                     /locus_tag="ACHOSM_RS08370"
                     /old_locus_tag="pgaptmp_001674"
     CDS             complement(4603..6651)
                     /gene="prc"
                     /locus_tag="ACHOSM_RS08370"
                     /old_locus_tag="pgaptmp_001674"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HGU4558221.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNVLWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6671..7369)
                     /gene="proQ"
                     /locus_tag="ACHOSM_RS08375"
                     /old_locus_tag="pgaptmp_001675"
     CDS             complement(6671..7369)
                     /gene="proQ"
                     /locus_tag="ACHOSM_RS08375"
                     /old_locus_tag="pgaptmp_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HGU4558222.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7466..7963)
                     /gene="msrC"
                     /locus_tag="ACHOSM_RS08380"
                     /old_locus_tag="pgaptmp_001676"
     CDS             complement(7466..7963)
                     /gene="msrC"
                     /locus_tag="ACHOSM_RS08380"
                     /old_locus_tag="pgaptmp_001676"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HGU4558223.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8093..9376
                     /gene="yebS"
                     /locus_tag="ACHOSM_RS08385"
                     /old_locus_tag="pgaptmp_001677"
     CDS             8093..9376
                     /gene="yebS"
                     /locus_tag="ACHOSM_RS08385"
                     /old_locus_tag="pgaptmp_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HGU4558224.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9345..11978
                     /gene="yebT"
                     /locus_tag="ACHOSM_RS08390"
                     /old_locus_tag="pgaptmp_001678"
     CDS             9345..11978
                     /gene="yebT"
                     /locus_tag="ACHOSM_RS08390"
                     /old_locus_tag="pgaptmp_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HGU4558225.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12058..13497
                     /gene="rsmF"
                     /locus_tag="ACHOSM_RS08395"
                     /old_locus_tag="pgaptmp_001679"
     CDS             12058..13497
                     /gene="rsmF"
                     /locus_tag="ACHOSM_RS08395"
                     /old_locus_tag="pgaptmp_001679"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HGU4558226.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFLGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPCELVRDGKLFTGNA"
     gene            13615..13851
                     /gene="yebV"
                     /locus_tag="ACHOSM_RS08400"
                     /old_locus_tag="pgaptmp_001680"
     CDS             13615..13851
                     /gene="yebV"
                     /locus_tag="ACHOSM_RS08400"
                     /old_locus_tag="pgaptmp_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HGU4558227.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13956..14147
                     /gene="yebW"
                     /locus_tag="ACHOSM_RS08405"
                     /old_locus_tag="pgaptmp_001681"
     CDS             13956..14147
                     /gene="yebW"
                     /locus_tag="ACHOSM_RS08405"
                     /old_locus_tag="pgaptmp_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HGU4558228.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14148..14804)
                     /gene="pphA"
                     /locus_tag="ACHOSM_RS08410"
                     /old_locus_tag="pgaptmp_001682"
     CDS             complement(14148..14804)
                     /gene="pphA"
                     /locus_tag="ACHOSM_RS08410"
                     /old_locus_tag="pgaptmp_001682"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU4558229.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHIPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15200..15541)
                     /gene="yebY"
                     /locus_tag="ACHOSM_RS08415"
                     /old_locus_tag="pgaptmp_001683"
     CDS             complement(15200..15541)
                     /gene="yebY"
                     /locus_tag="ACHOSM_RS08415"
                     /old_locus_tag="pgaptmp_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HGU4558230.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15554..16426)
                     /gene="copD"
                     /locus_tag="ACHOSM_RS08420"
                     /old_locus_tag="pgaptmp_001684"
     CDS             complement(15554..16426)
                     /gene="copD"
                     /locus_tag="ACHOSM_RS08420"
                     /old_locus_tag="pgaptmp_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HGU4558231.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16430..16804)
                     /gene="yobA"
                     /locus_tag="ACHOSM_RS08425"
                     /old_locus_tag="pgaptmp_001685"
     CDS             complement(16430..16804)
                     /gene="yobA"
                     /locus_tag="ACHOSM_RS08425"
                     /old_locus_tag="pgaptmp_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HGU4558232.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16943..17173
                     /gene="holE"
                     /locus_tag="ACHOSM_RS08430"
                     /old_locus_tag="pgaptmp_001686"
     CDS             16943..17173
                     /gene="holE"
                     /locus_tag="ACHOSM_RS08430"
                     /old_locus_tag="pgaptmp_001686"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HGU4558233.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17275..17931
                     /gene="yobB"
                     /locus_tag="ACHOSM_RS08435"
                     /old_locus_tag="pgaptmp_001687"
     CDS             17275..17931
                     /gene="yobB"
                     /locus_tag="ACHOSM_RS08435"
                     /old_locus_tag="pgaptmp_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HGU4558234.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17955..18617
                     /gene="exoX"
                     /locus_tag="ACHOSM_RS08440"
                     /old_locus_tag="pgaptmp_001688"
     CDS             17955..18617
                     /gene="exoX"
                     /locus_tag="ACHOSM_RS08440"
                     /old_locus_tag="pgaptmp_001688"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HGU4558235.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18614..20674)
                     /gene="ptrB"
                     /locus_tag="ACHOSM_RS08445"
                     /old_locus_tag="pgaptmp_001689"
     CDS             complement(18614..20674)
                     /gene="ptrB"
                     /locus_tag="ACHOSM_RS08445"
                     /old_locus_tag="pgaptmp_001689"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HGU4558236.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20883..21542)
                     /gene="yebE"
                     /locus_tag="ACHOSM_RS08450"
                     /old_locus_tag="pgaptmp_001690"
     CDS             complement(20883..21542)
                     /gene="yebE"
                     /locus_tag="ACHOSM_RS08450"
                     /old_locus_tag="pgaptmp_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGU4558237.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21869..22225)
                     /gene="yebF"
                     /locus_tag="ACHOSM_RS08455"
                     /old_locus_tag="pgaptmp_001691"
     CDS             complement(21869..22225)
                     /gene="yebF"
                     /locus_tag="ACHOSM_RS08455"
                     /old_locus_tag="pgaptmp_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HGU4558238.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22292..22582)
                     /gene="yebG"
                     /locus_tag="ACHOSM_RS08460"
                     /old_locus_tag="pgaptmp_001692"
     CDS             complement(22292..22582)
                     /gene="yebG"
                     /locus_tag="ACHOSM_RS08460"
                     /old_locus_tag="pgaptmp_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HGU4558239.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22716..23894
                     /gene="purT"
                     /locus_tag="ACHOSM_RS08465"
                     /old_locus_tag="pgaptmp_001693"
     CDS             22716..23894
                     /gene="purT"
                     /locus_tag="ACHOSM_RS08465"
                     /old_locus_tag="pgaptmp_001693"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HGU4558240.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23950..24591)
                     /gene="kdgA"
                     /locus_tag="ACHOSM_RS08470"
                     /old_locus_tag="pgaptmp_001694"
                     /gene_synonym="eda"
     CDS             complement(23950..24591)
                     /gene="kdgA"
                     /locus_tag="ACHOSM_RS08470"
                     /old_locus_tag="pgaptmp_001694"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGU4558241.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24628..26439)
                     /gene="edd"
                     /locus_tag="ACHOSM_RS08475"
                     /old_locus_tag="pgaptmp_001695"
     CDS             complement(24628..26439)
                     /gene="edd"
                     /locus_tag="ACHOSM_RS08475"
                     /old_locus_tag="pgaptmp_001695"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HGU4558242.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26674..28149)
                     /gene="zwf"
                     /locus_tag="ACHOSM_RS08480"
                     /old_locus_tag="pgaptmp_001696"
     CDS             complement(26674..28149)
                     /gene="zwf"
                     /locus_tag="ACHOSM_RS08480"
                     /old_locus_tag="pgaptmp_001696"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGU4558243.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28487..29356
                     /gene="hexR"
                     /locus_tag="ACHOSM_RS08485"
                     /old_locus_tag="pgaptmp_001697"
                     /gene_synonym="yebK"
     CDS             28487..29356
                     /gene="hexR"
                     /locus_tag="ACHOSM_RS08485"
                     /old_locus_tag="pgaptmp_001697"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HGU4558244.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29484..30926
                     /gene="pyk"
                     /locus_tag="ACHOSM_RS08490"
                     /old_locus_tag="pgaptmp_001698"
     CDS             29484..30926
                     /gene="pyk"
                     /locus_tag="ACHOSM_RS08490"
                     /old_locus_tag="pgaptmp_001698"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGU4558245.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31059..32030)
                     /gene="lpxM"
                     /locus_tag="ACHOSM_RS08495"
                     /old_locus_tag="pgaptmp_001699"
                     /gene_synonym="msbB"
     CDS             complement(31059..32030)
                     /gene="lpxM"
                     /locus_tag="ACHOSM_RS08495"
                     /old_locus_tag="pgaptmp_001699"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGU4558246.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32150..33472)
                     /gene="mepM"
                     /locus_tag="ACHOSM_RS08500"
                     /old_locus_tag="pgaptmp_001700"
     CDS             complement(32150..33472)
                     /gene="mepM"
                     /locus_tag="ACHOSM_RS08500"
                     /old_locus_tag="pgaptmp_001700"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HGU4558247.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33488..34420)
                     /gene="znuA"
                     /locus_tag="ACHOSM_RS08505"
                     /old_locus_tag="pgaptmp_001701"
     CDS             complement(33488..34420)
                     /gene="znuA"
                     /locus_tag="ACHOSM_RS08505"
                     /old_locus_tag="pgaptmp_001701"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HGU4558248.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDEDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34499..35254
                     /gene="znuC"
                     /locus_tag="ACHOSM_RS08510"
                     /old_locus_tag="pgaptmp_001702"
     CDS             34499..35254
                     /gene="znuC"
                     /locus_tag="ACHOSM_RS08510"
                     /old_locus_tag="pgaptmp_001702"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HGU4558249.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35251..36036
                     /gene="znuB"
                     /locus_tag="ACHOSM_RS08515"
                     /old_locus_tag="pgaptmp_001703"
     CDS             35251..36036
                     /gene="znuB"
                     /locus_tag="ACHOSM_RS08515"
                     /old_locus_tag="pgaptmp_001703"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HGU4558250.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(36185..37195)
                     /gene="ruvB"
                     /locus_tag="ACHOSM_RS08520"
                     /old_locus_tag="pgaptmp_001704"
     CDS             complement(36185..37195)
                     /gene="ruvB"
                     /locus_tag="ACHOSM_RS08520"
                     /old_locus_tag="pgaptmp_001704"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGU4558251.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37204..37815)
                     /gene="ruvA"
                     /locus_tag="ACHOSM_RS08525"
                     /old_locus_tag="pgaptmp_001705"
     CDS             complement(37204..37815)
                     /gene="ruvA"
                     /locus_tag="ACHOSM_RS08525"
                     /old_locus_tag="pgaptmp_001705"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGU4558252.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(37954..38019)
                     /gene="yobI"
                     /locus_tag="ACHOSM_RS08530"
                     /old_locus_tag="pgaptmp_001706"
     CDS             complement(37954..38019)
                     /gene="yobI"
                     /locus_tag="ACHOSM_RS08530"
                     /old_locus_tag="pgaptmp_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HGU4558253.1"
                     /translation="MYIFITHFFNEYVILKYLLPI"
     gene            38090..38692
                     /gene="yebB"
                     /locus_tag="ACHOSM_RS08535"
                     /old_locus_tag="pgaptmp_001707"
     CDS             38090..38692
                     /gene="yebB"
                     /locus_tag="ACHOSM_RS08535"
                     /old_locus_tag="pgaptmp_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HGU4558254.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQITAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(38694..39215)
                     /gene="ruvC"
                     /locus_tag="ACHOSM_RS08540"
                     /old_locus_tag="pgaptmp_001708"
     CDS             complement(38694..39215)
                     /gene="ruvC"
                     /locus_tag="ACHOSM_RS08540"
                     /old_locus_tag="pgaptmp_001708"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HGU4558255.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(39250..39990)
                     /gene="yebC"
                     /locus_tag="ACHOSM_RS08545"
                     /old_locus_tag="pgaptmp_001709"
     CDS             complement(39250..39990)
                     /gene="yebC"
                     /locus_tag="ACHOSM_RS08545"
                     /old_locus_tag="pgaptmp_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4558256.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(40019..40471)
                     /gene="nudB"
                     /locus_tag="ACHOSM_RS08550"
                     /old_locus_tag="pgaptmp_001710"
     CDS             complement(40019..40471)
                     /gene="nudB"
                     /locus_tag="ACHOSM_RS08550"
                     /old_locus_tag="pgaptmp_001710"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HGU4558257.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(40589..42361)
                     /gene="aspS"
                     /locus_tag="ACHOSM_RS08555"
                     /old_locus_tag="pgaptmp_001711"
     CDS             complement(40589..42361)
                     /gene="aspS"
                     /locus_tag="ACHOSM_RS08555"
                     /old_locus_tag="pgaptmp_001711"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGU4558258.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            42671..43237
                     /gene="yecD"
                     /locus_tag="ACHOSM_RS08560"
                     /old_locus_tag="pgaptmp_001712"
     CDS             42671..43237
                     /gene="yecD"
                     /locus_tag="ACHOSM_RS08560"
                     /old_locus_tag="pgaptmp_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU4558259.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            43234..44052
                     /gene="yecE"
                     /locus_tag="ACHOSM_RS08565"
                     /old_locus_tag="pgaptmp_001713"
     CDS             43234..44052
                     /gene="yecE"
                     /locus_tag="ACHOSM_RS08565"
                     /old_locus_tag="pgaptmp_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HGU4558260.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            44105..44500
                     /gene="yecN"
                     /locus_tag="ACHOSM_RS08570"
                     /old_locus_tag="pgaptmp_001714"
     CDS             44105..44500
                     /gene="yecN"
                     /locus_tag="ACHOSM_RS08570"
                     /old_locus_tag="pgaptmp_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HGU4558261.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            44541..45284
                     /gene="cmoA"
                     /locus_tag="ACHOSM_RS08575"
                     /old_locus_tag="pgaptmp_001715"
     CDS             44541..45284
                     /gene="cmoA"
                     /locus_tag="ACHOSM_RS08575"
                     /old_locus_tag="pgaptmp_001715"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HGU4558262.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            45281..46252
                     /gene="cmoB"
                     /locus_tag="ACHOSM_RS08580"
                     /old_locus_tag="pgaptmp_001716"
     CDS             45281..46252
                     /gene="cmoB"
                     /locus_tag="ACHOSM_RS08580"
                     /old_locus_tag="pgaptmp_001716"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HGU4558263.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQPHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(46417..48846)
                     /gene="torZ"
                     /locus_tag="ACHOSM_RS08585"
                     /old_locus_tag="pgaptmp_001717"
     CDS             complement(46417..48846)
                     /gene="torZ"
                     /locus_tag="ACHOSM_RS08585"
                     /old_locus_tag="pgaptmp_001717"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HGU4558264.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAVKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRNKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(48871..49971)
                     /gene="torY"
                     /locus_tag="ACHOSM_RS08590"
                     /old_locus_tag="pgaptmp_001718"
     CDS             complement(48871..49971)
                     /gene="torY"
                     /locus_tag="ACHOSM_RS08590"
                     /old_locus_tag="pgaptmp_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HGU4558265.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGFLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWASIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT        11847 a        12985 c        12290 g        12875 t
ORIGIN      
        1 agttttaatt tgaagcagtt gcaccatggc agtgcgtata atcaggatga attgagtaac
       61 ccttcaacga cggggttact caagattgag gaaggattat cgttcggcga ccagacgaat
      121 aatatcttcg tcttcaaccg atttttgact ctcaacggtc tttttatctg ctttctcagg
      181 ttcgtttgct tcgcacaaac gacgtaacag tgcattttga cgtttttgct gatccagcaa
      241 cgcctcaagc agttcaatct gttcgttagt ccgagaactg gcacgattga taaaaaacca
      301 caagatgagc ccgataagaa gaaccaccac cgatacaacc aaagacgcaa tgttcatcac
      361 gcctgaattt acaacttcgt tcatttcacc acctctgagt agaggcgcta ttctaccact
      421 gctggagagg aagaaaatct agtgctgaaa aaatgatatc accacgggat aaactggtta
      481 atggcacaaa ttccgctgaa aaattgtaca tcctgatcgc acatcatgtt gaatacctgc
      541 gcccaaagca gcaagcaagc caacaccacg acaaccagaa cgacccatcg aaactttttc
      601 actccaatct ccgcttcacc taccttatgt tattaaacta gcatgcgtta gttaaacagc
      661 gcctaactat gtcgatattc gtgcttttac ggaatgagta gcttgcttca gcgcatattg
      721 ccgctacgat taagcgaaca taaaaagaga agaggttgta atgcgattca tcattcgcac
      781 agttatgctg atagcactgg tatggatagg attattactc agcggatatg gcgttttgat
      841 tggaagcaaa gagaatgccg caggattagg tttgcaatgt acctatctga ctgccagagg
      901 caccagcacg gtacaatact tgcacactaa aagtggtttc ttggggataa cagattgccc
      961 actgttacgt aaaagcaata tcgtggtcga taatggttaa gggtaataaa taaccagatt
     1021 ctcactcatg agtaaaatat tcttatagca agcgcgttta atataacgca ccttcaactt
     1081 tatttttctc atgctaaata aagaacctgt ctgtttgata ttccgacagg ttcttactct
     1141 catcagaacg gatagtcgtg ataacccatt tgggcagaaa ttttgcgcgc tgcggtatgc
     1201 aacattgcga catattcctg taaacgctct tcagagaaac gcaacgtcgg gaaggagatg
     1261 ctcaaaccgg caatgaccac gccaaagcga tcaaataccg gtaccgcaat gcatcgcagc
     1321 ccttcttcct gctcttcgtt atcttcgcca tacccctgct cgcgcacctg gtccagaacg
     1381 ggtaataacg cttctgtact ggtgatggtc cgctcggtac tgcgtttata ctccacgccc
     1441 tcaagaattt gcttcacttc atcgcgatcg cgccatgcca gcagtacctt accaatcgcg
     1501 gtgctgtaca gcggattacg acgcccaatc cgtgaataca tgcgcaaatt gtacatagag
     1561 tcaattttgt gaatgtaaac aatactgtct tcgtccagtg cgccgaggtg gatagtttct
     1621 ttggtcaggc gggagatctc acgcatctgg atatctgcgc tacgaattaa atcgacgttt
     1681 tgtaacgcgc gagcgcccag ttcaaacaat ttcagggtca aggaatattt ctccgactcc
     1741 ccttcctgcg ccacataacc taaggttttc atggtctgta aaaagcgata aacggtgctt
     1801 tttgacatca tgacgcgctg cgacagctcg gttatcccta tttcgcgctc ttcacccagc
     1861 gcctgcaaaa tgccaaaaac ttttagcacg gaagatacag aatcaggctg tttatccaga
     1921 tctgcgttag ccatttatca cctcattgcg agtgttttat aaaaatcaga actgtttttt
     1981 attataattt cgcaccgggg tggtcgcaat ccatcttttg ccggttagtt acaattctgc
     2041 gacatccacc gtgaatatca gtgctagaat catacccctg ttgattattc accaaagata
     2101 taaaattcct atgccaaaag ttcaggccga cggcctgcca ttgccccagc gatacggtgc
     2161 gatattaacc attgtgattg gtatttcgat ggctgtcctt gacggcgcaa tcgccaacgt
     2221 cgccctgcca acaatcgcca cggaccttca tgccacgcca gccagttcca tctgggtagt
     2281 gaacgcctat caaatcgcca ttgtcatctc cctgctctcg ttttcgtttc tgggcgatat
     2341 gtttggctat cgacgtattt ataaatgcgg tctggtcgtt tttctgttgt cttcactgtt
     2401 ctgcgccctt tctgattcgc tgcaaatgct cacccttgcg cgtgtcatac aaggtttcgg
     2461 cggtgcagcg ttgatgagcg ttaataccgc acttatccgc ctgatctatc cacaacgttt
     2521 tctggggaga gggatgggca taaactcgtt tattgttgcc gtctcttctg ctgccgggcc
     2581 gacaattgct gcagcaatcc tctccatcgc atcctggaaa tggttatttt taatcaacgt
     2641 accgttaggt attatcgccc tgcttctggc gatgcgtttt ctgccaccca atggttctcg
     2701 cgccagtaaa ccccgtttcg acctgcccag cgccgtgatg aacgcgttaa ccttcggcct
     2761 gcttatcact gcgttgagtg gtttcgctca ggggcaatcg ctgacgttaa ttgctgcgga
     2821 actggtggta atggttgttg ttggtatttt ctttattcgc cgccagcttt ctcttcccgt
     2881 accgctgcta ccggtggatt tactgcgtat cccgctgttt tcactttcta tttgcacatc
     2941 tgtttgctct ttctgcgcac aaatgctggc aatggtttcc ctgccctttt acctgcaaac
     3001 cgtgctcggg cgtagtgaag tcgaaacagg tttacttctg acaccgtggc cgttagcaac
     3061 gatggtgatg gctccgttgg caggctattt gattgaacgc gtacatgcag gattgctggg
     3121 tgctctaggg ttgttcatca tggctgcggg gcttttttcc ctggttctgc tgcccgcgtc
     3181 acctgcggat atcaatatta tctggccgat gatcttatgt ggcgctggat ttggcttgtt
     3241 ccagtcaccc aataaccaca ccattattac ctccgctcct cgcgaacgta gcggtggagc
     3301 cagtggcatg ttaggaacgg ctcgtctact gggtcagagt agcggcgcgg cactggtggc
     3361 gctgatgcta aatcagttcg gtgataatgg tacgcacgtt tcgctgatgg ctgcggctat
     3421 tctggcagtg attgctgcct gtgtcagtgg tttacgtatc actcagccac gatccagggc
     3481 ataaaaaaag cgcgtcgatc aggacgcgct ttttagtatt tacttcatat tacttcaggt
     3541 attcacccgt acgcagagct tcaattcgtt tatccagcgg cgggtgagtc atgaacaact
     3601 cactgagcga tttcgactta ccgttaatgc agaaagccat catgctggtt gcttcttgcg
     3661 gttcatagct ggttttcagg cgctgcagcg cggcaatcat tttctcgcga ccaaccagtt
     3721 ttgccgaacc ggcatcagca tggaattcac gatgacgcga gaaccacatg gtgataatgc
     3781 tcgccagaat accaaacacc agttccagaa ccgttgcaac cgcaaagtag atcagcgggt
     3841 tgccgttgct ctcttcacct tcatcacgat ttccgcccat aaaacccgcg gcaagctgcg
     3901 ccagaatacg ggaaataaag ataacgaagg tgttcaccac gccctgaatc agcgtcatgg
     3961 tgaccatatc accattggcg atgtggctga tttcgtgagc aattaccgcc tcggcttcat
     4021 ccgggctcat gttctgcagc aaaccggtgc tgacagcaac cagagaggca tcacggcgcg
     4081 caccggttgc aaaagcgttg atgtccggcg catggtagat agccacttgc ggcatagcga
     4141 tccctgcctg acgagcctgg gttgctacag tattgaccag ccaacgttcc ctttcgttac
     4201 gcggttgctc gatcacttcc ccgccaacag atcgtaatgc catccatttg gacatcagaa
     4261 gcgaaacgaa ggaaccacca aaaccgaaca gcaaggccat gatcatcagc ccctgaacgc
     4321 tgctcgactg tatccctgtc aggctcagta ccagcccgaa aacgaccatt acggccaggt
     4381 tcgttagcag gaagagcgcg attcgcatca taattttctt tttacctcag tttaacaaaa
     4441 cgcaatatgc gatacccaca tcgtatgggt tacgcgacta ttttcaagtc tggatagtgc
     4501 gtaagtcacc agaaagacac aactttacat tttgtagcat ctgatttacg gcatcttgcc
     4561 gctgttaaaa aatcaggcac aatttcttgt gcctgattga tattacttga cgggagcggg
     4621 ttgttccgcg ggtctggctt tttcaagctt cgccagatcg agtgcgatat tcaccgtctc
     4681 atccagataa ggatccggct cctggtaatc tttcggtaga tcatccagct tcttcaactc
     4741 cggtttacct tcgcgtttaa agcgttcgtt caaacgcgcc agacgcgtcg catcatcttc
     4801 attattctct ttctcacgca cagcgtagtt cagagaaacg atattgcgct tgtccttcat
     4861 agcgttgaag cgcgcgatat ccttcatgat gttctggaac tcaggatctt tcgcgatacg
     4921 cgcattatgt tccttcagca gctccggccc aaaggccgtt aaatcgcctg atttcacata
     4981 agtcgcggca tcaatgctat cccacggcag cgcgttatct tcgaatttct cacccgtttc
     5041 cgtttcttca ttacccgtcg gcatgatgat gtctggcgtt acgcctttac gttgcgtact
     5101 gccgccgtta acgcgataga atttctggat cgtgtactgc acagaaccca gcgctggcca
     5161 ttcaggacgt aacatctgat cgtaaatacg gttcaatgaa cggtattgct gaacggtgcc
     5221 tttaccaaac gtcggttcac ccacaaccag cgcacgaccg taatcctgca ttgccgcggc
     5281 aaagatttct gaagccgaag cactgaaacg gtcaaccagt accaccagcg ggcctttata
     5341 gaacacctgc ccgtcggtat cgctgtcttc acgaaccttg ccgttgttat cgcggacctg
     5401 aacaatggga cccgcaggaa taaacagacc tgagagcgat accgcttcgg tcaacgcccc
     5461 accgccattg ctacgcaggt cgatgatcac actgctgaca ttctgttttt ccagtttctg
     5521 cagttgcact ttgacatcgt ctgtcaaacc cacatagaag cccggaatat ccagcacgcc
     5581 gactttctct ttaccgacgg tcttcaccga cattttaacc gcgcggtctt cgagacgaat
     5641 acgttcacgg gtcaatgtta cagtgcgggt cttggtccct ttaccagcag gtaaaatttc
     5701 cagacgaact ttactgccct tcggcccttt aattaaggca accacatcat caagacgcca
     5761 gccaatcacg tcaaccatcg gcttgcctgt ttgaccaaca ccgacaattt tgtcaccaac
     5821 gctgatagct ttactcttcg ctgccggacc acctgccacc atcgaattga taacggtgta
     5881 gtcatcatcc atttgcagca ctgcgccaat accttccagc gacaaactca tttcagtgtt
     5941 gaactgttcg gtattacgcg gggaaagata gttggtatgc gggtcgattt cacgcgcaaa
     6001 cgccgtcatt gccagcgaga aaacatcttc gctgttggtt tgcgccagac gacgaatggc
     6061 aaatttgtag cggcgtgtca gggtttcacg aatttcttta tccgtttttc ctgccagctt
     6121 caggcttaac tcgtcgaatt tgactttact gtcccacagc acgttcaact cagcctcgtt
     6181 tttcggccag ggcgctttgc tgcggtcaag gttataagtg tcgttgccgg tgaaatccat
     6241 cggcttttcc agtaccgaca aagcgtactg gtaacgctca aaacggcgct tttgcgccag
     6301 attgtagaga tcgtagaaaa cgtcgagttt gcctgaacgc agttcatcgc ctaactcggt
     6361 tttctttttc gcgaactgtt caacatcgct tgccaacagc acgttgtggc tgtaatcgag
     6421 cagattcagg tagcggtcaa agattttggc cgaaaatgcc tgatcgaggt cgaactggcg
     6481 ataatgagaa cgggtgaagc gcgacgttac gcgctcactc accgtcgcat gctgcgtctc
     6541 ttcctttaat accggaattt gatcagcacg cgtgatatct tctacagcga aggtctggcc
     6601 tgctattgca agcaggccag ctaacgcggt aagcctaaaa aacatgttca tgcctggccc
     6661 ggcctccgtt tcagaacacc aggtgttctg cgcgcacaat caaagacata cccgaattca
     6721 gctggacgcg gacgccgtct ttggtgattt ctaatacggt ggcatccatc gcgttttgac
     6781 ccgctttcac cttcagggct tgtccgacag tcagagctga aatgtcagaa actggggtgt
     6841 gctgttcttc gcgaggtgct tttgctgttt ttggcgcttt ctctaccggc ttttgcgcac
     6901 gaggtttacg ttcagcgcct tctttgcggc gtggcgtggt cggacgtggc ttgcgttcgc
     6961 gacgcggtgc gtcttctttc tcaccagcag ctgcggcagc ttcgcgtttt ttcgcttgct
     7021 gttcagcacg ctgtgcctga acacgcgctt tcgcttcttc aagctgcttg cgagcatgct
     7081 ctacatgttg ctcatccagc tcaccgcatg ggttgccgtc aagatcgaca cgcgttgcgc
     7141 cgggtttaac accgtaaaga taacgccagc tcgaagtgta gagacgtaaa gcggatcgta
     7201 attgcgtttt gctcaggttc atttccccgg caacacgatc gaccaaatcc tgaaaaatac
     7261 cgattttcag cggacgcgct tcaccttccg cactgaaaca gtggggaaaa cgttcggcca
     7321 gaaacgcgat tacttcttta ctgctattca acttaggttg attttccatg aaatttcctg
     7381 attacaacgg acgtagccaa caagcgcagg catgaacagg cgtcattata atgacgctac
     7441 cagtaaatgc tacgttatcc gttgattatc ctgcgacgct cgcaaagaat tttttgtaat
     7501 ccgtcgttgc aagcactttt tcaagctgtg ccacaagctg acgtaagcct tgctcgtcct
     7561 cgtctgtaaa gcgaccgaag acggtactat cgatgtcaag aacaccaata atctgatttt
     7621 tcaccaccag cggcagaaca atttcagaat tactcgccgc atcacaggca atatgcccgt
     7681 caaacgcgtg aacatcctcg atacgctgca cttgattgcg ggcaaccgca gtgccgcaca
     7741 ccccgcgccc gacgggtatc cggacacagg caattttacc ctgaaatggt ccgagtacca
     7801 gtgtatcgtc ctcaagcaaa taaaaacctg cccagtttac gtcagtgaga cgctcgtata
     7861 acaacgcact ggtgttcgca agcgttgcca gaaaactggt ttctcccgcc atcagcgcgt
     7921 taaagtcgcg atttaaatcc gcgtaaaatt ctgttttgtt cattatataa tcacctgttt
     7981 gtcttacctg gaactgccag cctattaaaa taagcattaa atgcgttaat gctcaagatc
     8041 attcccatca tgggttaaga ttaatgttaa ttcttattac atttggcacg tcatggctct
     8101 taacacacca caaattacgc cgacaaaaaa gataacagtg aggtcaatcg gcgaggaact
     8161 gccgcgtggt gattaccaac gttgcccgca atgtgacatg ctgtttagcc tgcccgagat
     8221 aaattctcat cagagtgcct attgtccgcg ctgtcaggca aaaattcgcg acgggcgcga
     8281 ctggtcgcta acgcgcctgg cggcaatggc tttcaccatg ctgttgctga tgccgtttgc
     8341 ctggggcgaa ccgctgttgc atatctggct gttaggtatt cgtatcgacg caaacgtcat
     8401 gcaaggcatc tggcaaatga ccaaacaggg cgatacgata acgggggcga tggtcttttt
     8461 ctgcgttatt ggtgcccccc tcattctggt gtcctccata gcatatttat ggtttggtaa
     8521 ccgactggga atgaatttac gtccggtact gctgatgctt gagcgtctta aagagtgggt
     8581 aatgctggat atctacctgg tcggcattgg cgttgcttct ataaaggtac aggattatgc
     8641 ccatatccag gcgggtgtcg gcttgttctc ttttgtggcg ttggtgattt taacgacggt
     8701 gacgttgtca catcttaatg ttgaggagct gtgggagcga tattatccgc agcgccccgc
     8761 tacgcgtagg gacgagaaac ttcgtgtctg tcttgggtgc cattttaccg gctatcctga
     8821 tcagcgtggt cgctgcccgc gttgccatat cccgctacgc ctgcgtcgtc gtcatagttt
     8881 gcaaaaatgc tgggcggcgc tgttagcgtc aatcgttttg ttgttaccag ccaacctatt
     8941 gcctatttct atcatttatc tgaatggtgg ccggcaggaa gatacgattc tttccgggat
     9001 tatgtcgctg gcaagtagca acattgcggt cgccggaatc gtgtttatcg ccagtattct
     9061 ggtaccgttt actaaagtga tcgtcatgtt cactttactg ttgagcattc attttaaatg
     9121 ccagcaaggt ttacgcacac gcattctgtt actgcggatg gtgacctgga ttggacgctg
     9181 gtcgatgctc gacctgtttg tcatatcttt aaccatgtcg ctgattaatc gcgatcagat
     9241 cctcgctttt actatgggac cggctgcgtt ttattttggc gcagccgtaa ttttgactat
     9301 tcttgctgtg gaatggctgg acagccgctt actttgggat gcacatgagt caggaaacgc
     9361 ccgcttcgac gactgaagcg cagattaaaa ataaacgccg tatctcacca ttctggctgc
     9421 tgcctttcat cgcgctaatg attgccggtt ggctgatttg ggacagttat caggaccggg
     9481 gtaataccgt caccatcgac tttatgtcgg cggatggtat tgtcccgggc cgtacgcctg
     9541 ttcgttatca gggcgttgaa gtcggaacag tgcaggatat cagcctcagc gacgatcttc
     9601 gtaagattga agtcaaggtc agcatcaagt ccgatatgaa agatgcgctg cgcgaagaga
     9661 ctcaattctg gctggtgacg ccaaaagcat cgttggcagg tgtctccggg ctggacgccc
     9721 tcgtcggtgg gaactatatc ggcatgatgc cgggtaaagg taaagagcag gatcactttg
     9781 tcgcactcga tacccaaccg aaatatcggc tggacaatgg cgatctgatg atccacctgc
     9841 aagcccccga tctcggttcg ctgaacagcg gttcattggt ctatttccgc aagatcccgg
     9901 tgggaaaagt ctacgactat gccatcaatc ccaacaaaca aggcgtggtg attgatgtcc
     9961 tgatcgagcg gcgttttacc gacctggtga aaaaaggtag ccgtttctgg aacgtttccg
    10021 gcgttgatgc caacgtcagt atcagtggcg cgaaggtgaa actggaaagt ctggcggcac
    10081 tggttaacgg tgcgattgcc ttcgattcac ctgaagagtc gaaacctgcc gaggcggaag
    10141 atacctttgg tctgtatgaa gatctggccc acagccagcg tggcgtaata ataaaactgg
    10201 aactgccgag tggggccgga ttgaccgccg actcgacgcc gttaatgtat caggggctgg
    10261 aagtcggaca gctaactaaa ctggatttaa atcctggtgg taaagtcact ggggaaatga
    10321 cagttgatcc cagcgtcgtt accctgcttc gtgaaaatac ccgcatcgaa ttacgtaatc
    10381 cgaaattatc ccttagcgat gccaatctca gcgccctgct gactggcaaa accttcgagt
    10441 tggtacccgg cgatggcgag ccacgcaaag agttcgttgt tgtgccaggc gaaaaagcac
    10501 tgctgcatga acctgatgtt ctgacgctga ccctgaccgc gccggaaagt tacggtattg
    10561 atgctggtca gccgctcatt cttcacggcg tgcaggtagg ccaggttatc gatcgtaaac
    10621 tcaccagcaa aggcgtcacc tttaccgtcg ccatcgagcc tcagcatcga gaactggtaa
    10681 aaggcgatag caaatttgtc gtcaacagcc gtgtcgacgt gaaggtgggg ctggatggcg
    10741 ttgagtttct cggtgccagc gcctcagaat ggattaacgg cgggatacgt attctgccgg
    10801 gcgataaagg cgagatgaaa gccagctatc cactgtatgc caatctggaa aaagcgctgg
    10861 agaacagcct tagcgattta cccaccacaa ccgtgagttt gagtgcagag acgctgccgg
    10921 atgtgcaggc aggatcggta gtgctctacc gtaaatttga agttggtgaa gttattaccg
    10981 tgcgtccgcg agctaacgcg tttgatatcg atctgcatat taagccggag tatcgcaacc
    11041 ttctgaccag caatagcgtg ttctgggcag aaggcggggc gaaagttcag ctgaatggta
    11101 gtggcctgac cgtacaggca tccccgctct ccagagcatt aaaaggggct attagcttcg
    11161 ataacctcag tggtgccagc gccagtcagc gtaaaggcga caaacgtatt ctgtatgctt
    11221 ccgaaacagc ggcccgtgcg gttggtgggc agattacgct tcacgctttc gatgccggaa
    11281 aactggcggt cgggatgcca attcgctatc tcggtattga tatcgggcaa atccagacgc
    11341 tggatctgat taccgcgcgc aatgaagtac aggcaaaggc ggtgctctat ccggaatatg
    11401 tccagacctt tgctcgcggt ggtacgcgct tctcagtggt cacaccgcaa atttcggcag
    11461 ctggcgttga gcatcttgat actatcctcc agccgtatat caacgtcgaa ccaggccggg
    11521 gcaatcctcg ccgcgacttt gaattacaag aggccaccat tactgattcg cgttacctgg
    11581 atggcttaag cattattgtt gaagcgccgg aagccggttc gttaggtatt ggtacgcctg
    11641 tgctgttccg tggtctggaa gtcggtacgg ttacaggaat gacgctgggg acattgtcag
    11701 atcgcgtgat gattgcgatg cgcatcagta aacgctatca acacctggtg cgtaacaatt
    11761 ccgtcttctg gttggcatcg ggttacagtc tggactttgg tctgacgggc ggcgtagtga
    11821 aaaccggcac ctttaaccag tttatccgtg gcggcatcgc cttcgccacg cctccgggta
    11881 cgccactggc accgaaagcc caggaaggca aacacttcct gttgcaggaa agtgaaccga
    11941 aagagtggcg tgaatgggga actgcgcttc ccaaataatg cccactgctc cggcgtgcct
    12001 gcgccggagc gtttatgcta aactgcgcgc ctgttttttt gccagtggta catgctcgtg
    12061 gcccaacaca ccgtttattt cccggacgcc tttctgacac aaatgcgcga agcgatgcct
    12121 tcgacgctct catttgatga ttttcttgcc gcctgtcagc gcccgttgcg ccgcagcatt
    12181 cgcgttaata cgctgaaaat ctccgttgct gatttcctgc aattaaccgc tccttatggc
    12241 tggacgctta cgccaattcc gtggtgtgaa gaaggtttct ggattgaacg cgacaatgaa
    12301 gatgcattgc cattgggtag taccgccgag catttaagcg gcctgtttta tattcaggaa
    12361 gccagttcaa tgttgcccgt tgccgccttg tttgctgacg ataatgcacc acagcgggtg
    12421 atggatgtcg cagctgcgcc aggctccaaa acgacgcaaa ttgccgcgcg gatgaataac
    12481 gaaggggcaa tccttgccaa tgagttttcc gccagtcggg taaaagtgtt acatgccaat
    12541 atcagccgct gtggcatcag taatgttgcg ctcacacatt ttgatggccg cgtgtttggt
    12601 gcggcagtgc cagaaatgtt cgatgccatt ttgctggacg ctccctgctc tggcgaaggc
    12661 gtggtgcgta aagatcccga tgcgctaaaa aactggtcac cagaaagcaa tcaggaaatc
    12721 gcagctacac aacgggagct tatcgacagc gcctttcatg cattacgtcc tggtggtacg
    12781 ctggtttact cgacctgtac cttaaaccag gaagaaaacg aagccgtttg cctgtggctg
    12841 aaagagactt accccgacgc agtagagttt ttaccacttg gcgatctctt ccttggtgca
    12901 aacaaagcgc tgaccgaaga aggctttttg catgttttcc cacaaattta cgactgcgaa
    12961 ggcttcttcg ttgctcgtct gcgtaaaact caggcgattc ccgccttacc cgcccccaaa
    13021 tacaaagtcg gtaattttcc gttcagcccg gtgaaagatc gcgaagctgg acaaattcgt
    13081 caggcggctg caggtgttgg cttaaactgg gatgaaaacc tgcgcctctg gcagcgtgac
    13141 aaagaactgt ggttgttccc ggtgggcatt gaagccctga tcggtaaagt ccgattttct
    13201 cgcttgggga ttaaacttgc cgaaacgcac aacaaaggtt atcgctggca gcatgaagca
    13261 gttattgccc ttgccacccc cgacaatgtg aacgcttttg aactgacacc gcaggaagcg
    13321 gaggagtggt atcgcgggcg cgatgtttac ccgcaagccg cgccagtggc ggatgacgtg
    13381 ttggttactt tccagcatca accgattggt ttagccaaac ggattggttc gcgattgaaa
    13441 aacagctatc cgtgtgaact ggtgcgcgat gggaaacttt ttaccggtaa cgcctgacag
    13501 cgcacaaaaa aagcgcactt tttgactggc acattcggct gcctcaacta ggctgaaaaa
    13561 tggtgcgatc ggactggtcg taccacaacc ggcagctaaa tggagagcac gaagatgaaa
    13621 accagtgtgc gcataggcgc ttttgaaatc gacgacggcg aattacacgg tgaatcgccg
    13681 ggtgatcgaa cgttaaccat tccttgtaaa tctgaccccg atttatgtat gcaactcgat
    13741 gcctgggatg ctgaaaccag tatccctgcc ctgctaaatg gcgaacactc tgtcctttac
    13801 cgtacccgtt acgatcaaca atctgatgcc tggattatgc gtcttgcctg atccaaaaag
    13861 aacccgtcgg catggcgggt tatttgtcct ggttattccc ccgttgtaaa atctctccta
    13921 aacttaacgg tacggcacca cacttcgggg atgaaatgtt cgcgctggta ctttttgttt
    13981 gctacctgga tggcggttgt gaagatattg ttgtggatgt ctacaacacg gaacagcagt
    14041 gtctttattc tatgagcgat caacggatcc gccatggcgg ttgttttccg attgaggatt
    14101 ttatagatgg tttctggcga cctgcacagg agtacggtga tttttaatta ttgcaattgc
    14161 acaagagtca gttcgccccc aaagacagca ccggtatcaa tataatgcag gttgccaata
    14221 tccacgcgat ggcgcaacgg tatatgacca aaccagaaat gatcagcacc tgtaattccc
    14281 tgcccttttt ggcgttcacc taatcgcgag cggctccaca agacctgatg caaatcaacg
    14341 tccttttgcc attcataaac atcatctgga taatcggcat gagcaataac atgtttgccg
    14401 gtacgactgt gtacttcaag aataaagggc aaatgctgac atttttccag cgccgttttc
    14461 gcttgtttct gttgattatc tgccagcgca ataaaccagt cgccgccatt catcaaccac
    14521 aaagacatct gctgggatgc cagcgcatcc atcgccatct gttcatgatt gcctcttacc
    14581 gcacaaaccc aatgttgttc cagtaactgc agacaacgta aactttgcgg cccacgatcg
    14641 ataacgtctc ccactgagat aagtaaatct cgccacggat caaaacgaca atgccataat
    14701 ttgcggcgca actgctcaag acaaccgtgt atatcgccag aaagccagat atgtcgccat
    14761 tgatgacccg caattctctg ataaacgggc gcaggctgtt tcatcaatat tttcctcccg
    14821 cgctaaagat cacataattt taacaagaat gttaaaaaac gctggactca gacagtagag
    14881 tgtgtgttat ggttgactat aaagtcagcg aaggaaatgc ttctggcttt taacagataa
    14941 aaagagaccg aacacgattc ctgtattcgg tccagggaaa tggctcttgg gagagagccg
    15001 tgcgctaaaa gttggcatta atgcaggctt agttgccttg ccctttaaga atagatgacg
    15061 acgccaggtt ttccagtttg cgtgcaaaat ggtcaataaa aagcgcggtg gtcatcagct
    15121 taaatgttaa aaaccgcccg ttctggtgaa agaactgagg cggttttttt attggaaatc
    15181 aaaaggctat tttaggtaat taacagagtt tttcagctcg ttctataaac ggtgccagac
    15241 tcattttttc gccgggattg ttaggatcat caatctgaat caccgaaatg ggttgggctt
    15301 tagtcttccc actggcaact tccttttgtg cgatatcgtt taaaggatac tgcacgaggg
    15361 tactcggatt aataacatac aaagcattac ccggtcggca agtcagcatc acctcttcgc
    15421 ggttaaatgc ccatttgtct ttacccactt caaaacgact gacggtaatc acctgcggtg
    15481 ccgccagcgc cgctgcagaa ctggtgagta acagaaacgc cagaatactt tttttcatca
    15541 ttttcgtaat ccatcaaaaa ggttcgagag tcgcaagcag gctgatgatc accagcacta
    15601 ccgcacctat cgcccattcg agtttagtca tccactcaaa ccagggtgcc gcacgatctt
    15661 catcctgcct catgcgcggt acaagaacat aacgattagc cagcgcaatg accaccataa
    15721 tcatcaccag gatggctttg agcaataaga gttggcccca gtaagtcgta agcgtggggg
    15781 gaaatccggt aatcaacaat gcattaagca cgccgcttgc cagtacgccg atcaccgcaa
    15841 aatgcccgca ccaggaaaaa cgcatcagcg cctgaatggc ctggtgtcgc caacggcctt
    15901 tgatgagttg catacaccag agcaccggca gtaatccccc aaaccacgcc gccgcacaaa
    15961 tcaggtgaat cgcgtgatta gtctgatgga ttttcgccgt taccccttca ttcagtgtcg
    16021 catgcccgat tcccgccagc agaataaatt gcgcggtagt gagcataaac agcaaacgtg
    16081 gcatattccg cggttgcata agggcgacga tcaacgttac gagggcgaga acaatttgcc
    16141 ataaccagac gccaccaaac tgcgtttgca aaacagcctg ccaaatgttt ggcgaaaata
    16201 catctgccca tcccgttccc atcaacccgc cctgaacggc gagcattgca gtggcactga
    16261 tcaaactcca gacggcagcg tgctgttgta aacgcagaaa acgcttcgcc agcaagcgac
    16321 gaatcgttaa gggggccagc caggcaccgt acattgcgaa gccaaagacc agcatcagcg
    16381 aggtgaaatg gataaatcgc agcgcgatcc aggtaaacgc cagcatgatt tatttcacgc
    16441 taaaggtgta gtgtcctttc gttttatgcc cgtccaccga aacaacatgc cagtcaacgg
    16501 tgtaggttcc gggtttcagc gaatcggcca gtggaacaat cagttgcttt tggtcctgct
    16561 cattccgctt cgctggtaat gttttaatat tttcgttttt tggccccgtg atttttgcac
    16621 cactgaatcc tgtttcaaca ccttccgaga agtttaaagt gattgcctgc ggtgcagctg
    16681 tcacttgcgc gtttgccgca ggatactgat gcgttaaatg cgcatgtgcc cagacggaag
    16741 gtgtcaccaa cgaagtggtc aggattgcca gcgcgtagcg aagggagcgt gcagttgaag
    16801 ccatattatc tattcctttt tgtaataact tttttacaga gcataacctt gtctaatgtc
    16861 tgagtcgagg atcatcaatt ccggcttgcc atcctggctc actcttagta acttttgccc
    16921 gcgaatgatg aggagattaa gaatgctgaa gaatctggct aaactggatc aaacagaaat
    16981 ggataaagtg aatgtcgatt tggcggcggc cggggtggca tttaaagaac gctacaatat
    17041 gccggtgatc gctgaagcgg ttgaacgtga acagcctgaa catttgcgca gctggtttcg
    17101 cgagcggctt attgcccacc gtttggcttc ggtcaatctg tcacgtttac cttacgagcc
    17161 caaacttaaa taaaacttat acagggttac actttcttac ataacgcctg ctaaattatg
    17221 agtattttct aaaccgcacc cataatttgc agtcattttg aaaaggaagt cattatgtcg
    17281 ttctggaaag ttgcagcagc gcaatatgag cctcgtaaaa cctcactcac cgaacaggta
    17341 gctcatcatc tggagtttgt gagggccgcc gccaggcaac aatgtcagct gttagttttt
    17401 ccatcgcttt ctttactggg gtgtgattat tcgcgacgtg cccttcctgc cccacccgat
    17461 ctctcactgt tagacccgct ttgttatgcc gcaacgacct ggcgaatgac catcattgcc
    17521 ggccttcccg ttgaatataa cgatcgcttt attcgtggaa ttgcggtgtt cgcaccgtgg
    17581 cgaaaaacgc ctgggatcta ccatcagagt catggtgcat gtctgggccg tcgttccaga
    17641 accatcacgg tggtcgatga acaaccggaa ggtatggata tggacccgac ctgttcactg
    17701 tttacgacag ggcaatgtct gggagaaccc gacctgctgg cgtcggcccg tcgcttacaa
    17761 tttttttcac atcagtactc gattgctgtg ctgatggcaa atgcccgtgg taacagtgca
    17821 ctatgggatg aatatggtcg tctcatcgtt cgcgccgatc gtggttcatt attgttagtc
    17881 ggtcagcgtt catcacaggg ttggcaaggc gatatcattc cattacgcta ggctttttcg
    17941 gcctggagca tgccatgttg cgcattatcg atacagaaac ctgcggtttg cagggaggga
    18001 tcgttgagat tgcctctgtt gatgtcattg acggaaaaat cgtcaacccc atgagccacc
    18061 tggtgcgccc cgatcgtcct attagtccac aagcgatggc gattcatcgc atcaccgaag
    18121 ccatggtcgc cgataaaccg tggattgaag atgtgatccc acactattac ggcagtgaat
    18181 ggtatgtcgc gcacaacgcc agctttgacc gccgcgtact gcctgagatg cccggtgagt
    18241 ggatttgcac catgaaactg gcccgtcgct tgtggccagg aatcaagtac agcaatatgg
    18301 cgttatataa aacacggaag ctcaatgtac agacgccgcc gggcctgcat catcaccgcg
    18361 cgttgtatga ctgttatatc accgccgcgt tgcttatcga tattatgaac acctccggct
    18421 ggacggcaga acagatggcc gatatcaccg gacgtccgtc gttgatgacg accttcacct
    18481 ttggcaaata ccgtggcaaa gcggtttccg acgttgccga acgcgatccg ggctatctgc
    18541 gctggttatt taataacctg gacagcatga gcccggagct gcgtttaaca ctgaaacatt
    18601 atctggaaaa tacttaatcc gcaggcgtag cgggtaatgt tccctgcgcc agcgcgacca
    18661 gaaaagcata ttccatcgct acgccttcgt acgatttaaa gcggccagat ttaccgccat
    18721 ggcctgagtc catgtcggta cagagcaata aaagatggtc atcggttttc agctcgcgca
    18781 atttagcgac ccattttgcc ggttcccaat attgcacctg agaatcgtgt aaaccggtcg
    18841 ttaccagtaa atgcggataa gcctgtgcgg tgacgttgtc atacgggctg tagcttttca
    18901 tgtactcgta atattgcgga tcctgcgggt taccccactc ttcaaactca ccagtggtaa
    18961 gaggaattga ttcatcaagc atcgttgtta caacatcaac aaacggtacc tgggcgataa
    19021 cgccgtggaa taattccggg cgttgattaa ttgcaacgcc catcaacatg ccccccgcac
    19081 tcccgcccat cgcataacaa agcgaaggag agccatagcc cagttttaac aatgcatcgc
    19141 aggcatcaag ataatcatta aacgtatttt tcttcttcag aaattttccg tcttcgtacc
    19201 attgttgccc cagctcacca ccgccgcgaa catggacaat agcgtagaca aagccacgat
    19261 ctaacaaact caagcggcta aaactgaaat cggcatcaat acttgcgccg taagaaccat
    19321 agccatacac cagcaacggg ttgtgtcctt tgcgaaaatg tttgcgatgg tagaccaacg
    19381 aaaccggaac ttcgacgcca tcacgggcga ctatccacag gtgttcactg cggtaattcg
    19441 ccgcatcaaa accaggaact tccgtttgtt ttaatacacg acgctcaccg gtatccatat
    19501 ccagttcaaa caaagtgtct ggtgtggtca tggaagaata accataacgc aatcgcgcgg
    19561 tttcaggttc tggattgtag gcaatccagg tcacataggc tggatcatca aaggcaatac
    19621 caatgacttc ccgggtcttg cggttaattt ggcgcaaact ggttaacccg cgctgacgct
    19681 cttcaaccac cagccagtcg gtaaacagcg taaacccttc cagcatgatg ttttcgcgtg
    19741 gcggaattaa ctcttcccac tgttgctcat cacgcatacg ggtacggtat aagccaaagt
    19801 ttttgccgtg gcggttggaa cgcagataaa aacgatgctg gtagtgatca aggctgtatt
    19861 cgtgatcttt gcggcgcggc agaaaaacaa acggctcggc atcggccatt tccgcgtcca
    19921 gcaggcgaac ttcactggtg gtggcgctgg ccaaatgaat gactacatag tgcttcgagg
    19981 tcgttttatg caggctgacg taataggtat cgtctttttc ttcgtagatc agtttatctt
    20041 gcgatgctgg cgtaccgatg gcgtgacgcc agacctgata aggcagcagc gtcaccggat
    20101 gcttgcgaac atagtagaaa gtccaggagt catttgccca gacaaagctg ggttcaacgt
    20161 tatccagcag ttccgggtac cagttaccag tttccagatt acgaaaacga atgccgtact
    20221 ggcgtcggga aagaaaatct tctgccagcg ccataatggt gttatcgggc gtaatcgcca
    20281 ttccgcccat cgaataaaac tcactatgag ctgcgcgctt attggcatcg agcaatgttt
    20341 cccactcatc ccactcttca ctgaatgccg attgacgctg gtagatagca tattcacagc
    20401 ctggttcata aatatgccga tagcggtagc catttttgat gtagggcgca gaaacttctc
    20461 gttgcggaat gcggtcgatg atttccttta agatgcgatc ctgcaaggct tgttgtgagg
    20521 ccatcacccg atgaccgtaa ctattttctt gttgcaggta gtccaggact tctggctgag
    20581 aacgcgtatc gtcccgcagc cagtagtaat tatcgatgcg cgtatcgcca tgaagcgtca
    20641 tggcgtgggg aatgcgggcg gcttttggta gcatgttatt gttctttctg gttgaaacat
    20701 cttataaggg tggcaaaact caccggggat gcaagcgaaa cagggtcgtc attgcttaga
    20761 tgatgacagg taatggcgcg gatatcgaat gttatgcaga cagagaaaat cagcctgttc
    20821 aaatggctgt gcggttctgg atagcccgaa atagtcaact tcaggctatc cagagagcgg
    20881 aattattccg ccaaagtgcg tttttgctgt tcgagatcgc gttcaatgcc gtcacgaaca
    20941 tcctggggga ttttcagcgc gtcacccagt gcattcaggt aactgcgttc cataaaatgg
    21001 tcaatatcaa tagccgcgca actcaggaaa tagatttcca gcgcctcttc ttcattgcgg
    21061 accccggtag ccaggcgttg tggatccagc ggttgttcga ttgcctgctc aatgagtaca
    21121 cgcccctgct cttccacgcc ggcttcacgc aattgctggt cgattgccgc acgttctttg
    21181 gcatcaatat gaccatcact tttagcggca aagaccagcg caaggatcaa acgttccgta
    21241 cgctcatcca gcggcgtact ttgcgcgcca aactgcggtt cgtcctgatg tgccgcgcga
    21301 attttatctt tgtatttatt ccacagcacc gtacccgcta ccgctccgcc gccaaccagt
    21361 aacgcgttgg tgccatattt cgtaagaagt ttacgtgctg atttatttgc gaccagcagc
    21421 ccagccagtc cgcctaatgc gcccggcact aaaagtttgc ccaatccctg atccgcagac
    21481 gaggaggtag aagaactgct ttgcccaagc agggattgca gttgatttaa ccagttagcc
    21541 atatttgctc ctcaataacc atttttagtg cctgtagcat agcggatgag gatgtcagaa
    21601 aatgtatatc gagacaaaag atgcgcaaat aagcggggcg gtaggccgtt attcaaagaa
    21661 attcgcgcca ggtaaggtat ttactgtata tcaggtgata agggtatttt aggtgaaagt
    21721 agtttgtcgt tttgagtggc attgtttgat gctgattgcc cgatgcgacg ctgttgcgtc
    21781 ttctcaggcc tacgattgtg gtgcgttctg gggcggacaa ggcactcgcg ccgcatccgc
    21841 caacggtcaa tcgaattatc agaagccatt aacgccgctg atattccgcc attcccgctt
    21901 tgcagtccac gctgacctgg taatgaatat cggcactttt accacgcacg gttagcggta
    21961 ctgaccattt atcatcttta ccctgaatgt cctgcaaact gacccaagcc acgggatcgg
    22021 cctgaccgac aattttttga tcatctgccc aacgcgccac gcgattttgt tgataatcac
    22081 gttttacgct cgcggcaatt ccggcagcat ccagaccttc acactttggg aaagtgaccg
    22141 acttgctggt ttcattattg gcagcgaaaa ctgatgcgca ggcagaaacc aacaacagcc
    22201 ctaaaaacgc ccctcttttt ttcatgtttt tctccatagc acaatgattc aggagaaagc
    22261 atggtacaaa ttgtcaggag cgcaagttgc ttcaggccgc gtgtgaggca tcttcctctt
    22321 cggattcagc accgacgacc tgtggggacg gtaatttgcc ggttttcaga atggtgctca
    22381 gcacatcttt ttgttctgcc agccatagcg aaagggcttc acgttgctcg tcttccattt
    22441 gcactggaga atttgtcagc caggtgtcaa gcagatccgc cgtatcaagc attttgtcat
    22501 aagcatcggc ttcctttttg ctggtaaacg acattttctc ttcgccctca cgaatgacta
    22561 cgtatttaac ttcaaccgcc atttgcagcc tctcataata actgtgactt tatacagtat
    22621 atttcttttc ggttgagaaa tcaacatcag caataaagac acacgcaaac gttttcgttt
    22681 atactgcgcg cggaattaat caggggatat tcgttatgac gttattaggc actgcgctgc
    22741 gtccggcagc aactcgcgtg atgttattag gctccggtga actgggtaaa gaagtggcaa
    22801 tcgagtgtca gcgtctcggc gtagaggtga ttgccgtcga tcgctatgcc gacgcaccag
    22861 ccatgcatgt cgcgcatcgc tcccatgtca ttaatatgct tgatggtgat gcattacgcc
    22921 gtgtggttga actggaaaaa ccacattata tcgtgccgga gatcgaagct attgccaccg
    22981 atatgctgat ccaacttgaa gaggaaggac tgaatgttgt cccctgcgct cgcgcaacga
    23041 aattaacgat gaatcgcgag ggtatccgtc gcctggcggc agaagagctg cagctgccca
    23101 cttccactta tcgttttgcc gatagcgaaa gccttttccg cgaggcggtt gctgacattg
    23161 gctatccctg cattgtaaaa ccggtgatga gctcttccgg caaggggcag acgtttattc
    23221 gttctgcaga gcaacttgct caggcatgga agtacgctca gcaaggcggt cgcgccggag
    23281 cgggccgcgt aattgttgaa ggcgtcgtta agtttgactt cgaaattacc ttgctaaccg
    23341 tcagcgcggt ggatggcgtc catttctgtg caccggtagg tcatcgccag gaagatggcg
    23401 actaccgtga atcctggcaa ccacagcaaa tgagcccgct tgcccttgaa cgtgcgcagg
    23461 agattgcccg taaagtggtg ctggcactgg gcggttatgg tctgtttggt gtcgagctgt
    23521 ttgtctgtgg tgatgaggtg attttcagtg aggtctcccc tcgtccacat gataccggga
    23581 tggtgacgtt aatttctcaa gatctttcag agtttgccct gcatgtacgt gccttcctcg
    23641 gacttccggt tggcgggatc cgtcagtatg gtcctgcagc ttctgccgtt attctgccac
    23701 aactgaccag tcagaatgtc acgtttgata atgtgcagaa tgccgtaggc gcagatttgc
    23761 agattcgttt atttggtaag ccggaaattg atggcagccg tcgtctgggt gttgcgctgg
    23821 cgaccgctga gagtgttgtt gacgccattg aacgcgcgaa gcacgccgcc ggacaggtaa
    23881 aagtacaggg ttaaacccgg caaaaaaacg ctacaaaaat gcccgatcgt agatcgggcg
    23941 ttttgacttt tacagcttag cgccttctac agcttcacgc gccagcttag taatgcggtc
    24001 gtaatcgccc gcttccagcg catctgccgg aaccagccag gaaccaccga tgcacagcac
    24061 gcttttcagc gccaggtagt cacggtagtt agccggagaa ataccacccg tcgggcagaa
    24121 acggacctgg gagaacggac ccgcgatcgc ctgcagggct ttcacgccgc cgttagcttc
    24181 agccgggaag aatttgaact ctttcaaacc gtagtccata cccagcatca gttcggaaac
    24241 agtgctgatc cccggaatta gaggaatagt cccttcggta gcagctttca gcagcggctc
    24301 ggtcagaccc gggctaattg cgaactgtgc acccgcttca gtgacttctg ccagctgctg
    24361 tggattcagc accgtaccgg cacccacaat cgcttcaggc acttctttgg cgatagcacg
    24421 gatagcgtca actgcacact cggtacgcag agtcacttcc agaacgcgca ccccaccagc
    24481 aaccaacgct tttgccatcg gcactgcgtg ttccagtttt tttaccacga taaccggtac
    24541 aaccgggccg gtggtcagga ttgattctgc acttgttttc cagtttttca tcagagtttt
    24601 ctctcgcctg attacaaatt tgtcgtctta aaaagtgata caggttgcgc cctgttcggc
    24661 accggacagt ttttcacgca aggcgctgaa taattcacgt cctgtcccca cgcgtgacgc
    24721 gctcaggtca ggaatgtgcg gttcgcgagc agccagttcc gcttcgtcta ccagcagcgt
    24781 cagttcgcct gtctgtccat tcacacgaat gatgtccccg tcgcgcactt ttgccagcag
    24841 cccgccatcg taggcttctg gtgttacgtg gatagctgac ggcactttac ctgaagcgcc
    24901 ggagagtcgt ccatcggtaa ctaacgcaat tttgaaacac cggtccaata atacaccaag
    24961 tggcggcatg agtttatgta attctggcat tccgttcgct tttggcccct gatgacggac
    25021 aacaacgaca caatcgcggt ccagcaaacc cgcttcaaag gccggcataa cgtcatgctg
    25081 gctttcaaaa acaaccgctg gcgcttcaat cacctggttc tcaaccggca cggcagaggt
    25141 tttcataacc gcacggccca ggttaccgct taacactttt gtcccaccat gatgagagaa
    25201 aggttgttcg aaggaagcga tcacattgct gtcgagtgat ttttccgccc cttcccgcca
    25261 gtccagttca ccattattca gccatggttc aagggtataa cgagacagac caaagcctgc
    25321 caccgtattg acatcttcat gcagcaggcc tgctttgagc agttcacgca ccagaaccgg
    25381 tacgccacct gccgcctgga agtggttaat atcggccgga ccgttcgggt agagacgtgc
    25441 catcagcggt acaacatcag aaaggtcaga gaagtcatcc cagttaatct gaataccggc
    25501 cgcgcgcgcc atcgccacca ggtgcatggt gtggttagtg gaaccaccgg tcgccagcag
    25561 tgcaacgata ccgttcacca ccactttctc atcgatcatc ttaccgatcg gcatccattc
    25621 attaccatta ccggtcatgc gtgtaacctg acgcgcagcg gcagcggtca aagcatcgcg
    25681 cagcggagaa tccggatgaa caaaagaaga gcctggcaac tgcatcccca taaactccac
    25741 caccatctgg ttggtgttgg cagtaccgta gaaagtacat gttcccggcg catgataaga
    25801 cgcggcttct gactccagta aggccatgcg gtccacttta ccttcggcat aaagctggcg
    25861 aatacgcact ttttctttat ttggcaaacc gcttgccatc ggtccagacg gcacaaacac
    25921 cgcaggcaaa tgaccaaacg acagggctgc catcgtcaga cccgggacaa tcttgtcgca
    25981 caccccgagg aacagagcac catcaaacat gttatgggac agcccgaccg ccgcagacat
    26041 cgctatcact tcgcggctta gcagcgacaa ttccattcca tcctgcccct gggtgacacc
    26101 atcacacatc gccggaacac cgcccgcaac ctgaccaacc gcattcgctt catgcagggc
    26161 tttacgaatg atttctggat agtgttcata aggctggtga gcggagagca tgtcgttata
    26221 ggaggtgatg atggcgatat tgttacgcaa catgcttttc aaagaggctt tgtcttctgg
    26281 ctggcaggca gcgaaaccgt gtgccaggtt accgcatgcc aactgcgaac gatgaacggt
    26341 cgacgttttc gcttgttcta tccgggcgag ataagcagag cgagtctcgc gcgaacgttc
    26401 aatgattcga tttgttacgc gtaacaattg tggattcata aaggctcctg aaattgagtt
    26461 gtcagagcag gatgattcac aacgcgtttc attcagagga tttatgagtg aaacgcctgt
    26521 aaccggagct catagggcaa aaacgtttca gtcagtgtaa taaaaaaagc ctcgtgggtg
    26581 aatccgcacg aggcctgaaa gtgtaaaaat tgttctacaa tctgtgcaag atcatgttac
    26641 cggtaaaata accataaagg ataagcgcag atattactca aactcattcc aggaacgacc
    26701 atcacgggta atcatcgcca ccgaggcaac gggtccccag gttccggcct gatacggttt
    26761 cggcgcatca ttgtccatcg cccacgcctc agtaatggag tctacccatt tccaggcttc
    26821 ttccacttcg tcgcgacgta caaacagtgc ctgaatacca cgcatggttt ccagcagcaa
    26881 acgttcatag gcatccgcca gatgcgtctg attaaaggtt tctgaatagc tcagatccag
    26941 cttggtgatt tgcaggttat gtttgtggtc aaggccagga actttattca gtacctggat
    27001 atccacgcct tcatcaggtt gcagacggat agtcagttta ttctgcggca gatcctgcca
    27061 cgactcttta aacagattca gttcaggtgt tttgaaatag accacgactt cagaacattt
    27121 ggtcggcaga cgtttaccag tacgcaggta gaatggcaca ccggcccagc gccagttatc
    27181 aatgtcgacg cggatcgcca cgaaggtttc tgtattgctg ctcttgttcg cgccctcttc
    27241 ttccagatat cccggcactt ttttgccctg ggcgaagccc gcagtatatt gcccgcgtac
    27301 ggttttttca cgtacgttgg agcggtcaat gcggcgcaga gacttcagta ctttaacttt
    27361 ttcatcgcgg atgctgtctg cactcaaatc cgacggcgga gacatcgcaa tcatgcagag
    27421 aatttgtagc aggtggtttt ggatcatatc gcgcatctga cctgctttat caaaatagcc
    27481 ccagcgccct tcgatcccca cttcttctgc cacggtaatc tcaacatgat caatggtgcg
    27541 attgtcccag ttattcacaa acagggagtt agcaaaacgc agcgccaaca ggttcagcac
    27601 cgtttcttta ccaagatagt ggtcgatacg gtaaacctgg cactcctcga agtattcgcc
    27661 aacctgatca ttgatttcct gcgaggtcgc cagcgacgtc cccagcggtt tctccatgac
    27721 tacgcgtgcc ggtttagcat tcagttttgc ctcgccaagc cctttgcaaa ttgcgccaaa
    27781 agtgctgggc ggcatggcaa agtagttaat ggtgatacga tttttttgat ccagcatcgc
    27841 gccgagacgg ctgaatgcag cagtgtcatt gacatcgaga ttacaaaaat ccagacgtgc
    27901 actcagggtg tcccataaac cttcatcaat ggtttctttc atgaaagttt cgagcgcctc
    27961 gcggacaact ttggtatatg ccgctttatc ccagtcagca cgccctacgc cgataatccg
    28021 ggtgtccggg ttgagctgac cggctttttc cagttgatac agggaaggca gcaatttacg
    28081 acgcgcaagg tcgcctttcg cgccgaaaat gaccaggtca caggcctggg ctgtttgcgt
    28141 taccgccatg tcattctcct taagttaaat aacccggtac ttaagccagg gtatacttgt
    28201 aattttctta cggtgcactg tactgctttt acgagcttgc gaaaactgta aacgcttatc
    28261 cacccgtgcg attacggtaa aagcgcgtaa agtgcggcaa aactgataaa aaaatcatcg
    28321 ttttccattg ggtaaaaatc tgacactgat catgttatga aaaaaaataa caactttttt
    28381 atctgctttt gtcattaaca cagcacacag gcgtaatatc tgacaaaact gcatttcgat
    28441 ttctttcagt gcggaaatcg tcattacccg tgagtctctt tacatcatga atatgctgga
    28501 aaaaatccag tctcagctgg aacatttgag caaatcggag cgcaaagttg ccgaggtcat
    28561 tctggcttcg cccgataacg cgatccattc gagtattgct gctctggcgc tggaagccaa
    28621 tgttagcgaa ccgacggtga atcgtttctg tcgcagcatg gacacgcgcg gttttcctga
    28681 ttttaaactt catctggcac aaagtctggc gaatggcact ccctatgtta atcgcaacgt
    28741 caatgaagat gacagcgttg aatcatacac ggggaaaata tttgagtccg caatggcaac
    28801 gctcgatcat gtccgtcatt cactggataa atctgccatc aaccgcgccg tcgacttgct
    28861 cactcaggca aaaaaaatcg cctttttcgg attaggctct tcagccgccg ttgcccacga
    28921 tgcgatgaat aagttctttc gttttaatgt tccggtggtg tactccgatg atatcgtgct
    28981 gcaacgcatg agttgtatga attgtagcga cggagacgtg gtggtgctga tttctcacac
    29041 tggaagaaca aaaaatctgg tcgagctggc gcagctggca cgcgaaaacg acgctatggt
    29101 gattgccctc acctctgcgg gtaccccgct cgcccgggaa gcaacgctgg caattaccct
    29161 cgacgtaccg gaagatactg acatttatat gcccatggtt tctcgacttg cacagctgac
    29221 cgtgatagat gtgctggcga caggatttac tttgcgacgc ggtgcaaaat tcagagataa
    29281 cttgaagcgg gtcaaggaag cgctgaagga atcgcgtttt gataagcagt tacttaattt
    29341 aagtgacgat cgctaaaagc gactgtcact gttctaatct tatacgacat ccgaatgaga
    29401 ttaatttatc gccatcgcgg cgttatttca ttcggatttc atgttcaagc aacacctggt
    29461 tgtttcagtc aacggagtat tacatgtcca gaaggcttcg cagaacaaaa atcgttacca
    29521 cgttaggccc agcaacagat cgcgataata atcttgaaaa agttatcgcg gcgggtgcca
    29581 acgttgtacg tatgaacttt tctcacggct cgcctgaaga tcacaaaatg cgcgcggata
    29641 aagttcgtga gattgccgca aaactggggc gtcatgtggc tattctgggt gacctccagg
    29701 ggcccaaaat ccgtgtatcc acctttaaag aaggcaaagt tttcctcaat attggggata
    29761 aattcctgct cgacgccaac ctgggtaaag gtgaaggcga caaagaaaaa gtcggtatcg
    29821 actacaaagg cctgcctgct gacgtcgtgc ctggtgacat cctgctgctg gacgatggtc
    29881 gcgtccagtt aaaagtactg gaagttcagg gcatgaaagt gttcaccgaa gtcaccgtcg
    29941 gtggtcccct ctccaacaat aaaggtatca acaaacttgg cggcggtttg tcggctgaag
    30001 cgctgaccga aaaagacaaa gcagacatta agactgcggc gttgattggc gtagattacc
    30061 tggctgtctc cttcccacgc tgtggcgaag atctgaacta tgcccgtcgc ctggcacgcg
    30121 atgcaggatg tgatgcgaaa attgttgcca aggttgaacg tgcggaagcc gtttgcagcc
    30181 aggatgcaat ggatgacatc atcctcgcct ctgacgtggt aatggttgca cgtggcgacc
    30241 tcggtgtgga aattggcgac ccggaactgg tcggcattca gaaagcgttg atccgtcgtg
    30301 cgcgtcagct aaaccgagcg gtaatcacgg cgacccagat gatggagtca atgattacta
    30361 acccgatgcc gacgcgtgca gaagtcatgg acgtagcaaa cgccgttctg gatggtactg
    30421 acgctgtgat gctgtctgca gaaactgccg ctgggcagta tccgtcagaa actgttgcag
    30481 ccatggcgcg cgtttgcctg ggtgcggaaa aaatcccgag catcaacgtt tctaaacacc
    30541 gtctggacgt tcagttcgac aatgtggaag aagctattgc catgtcagca atgtacgcag
    30601 ctaaccacct gaaaggcgtt acggcgatca ttaccatgac cgaatcgggt cgtaccgcgc
    30661 tgatgacctc ccgtatcagc tctggtctac caattttcgc catgtcgcgc catgaacgta
    30721 cgctgaacct gactgctctc tatcgtggcg ttacgccggt gcactttgat agcgctaatg
    30781 acggcgtagc agctgccagc gaagcggtta atctgctgcg cgataaaggt tacttgatgt
    30841 ctggtgacct ggtgattgtc acccagggcg acgtgatgag taccgtgggt tctactaata
    30901 ccacgcgtat tttaacggta gagtaagtac gttgccggat acggcgaaaa cgccacatcc
    30961 ggcctacagt tcattgatag ttcaacagat ttcgaatatt ctgaagcaaa cttggactta
    31021 tcatcaagcg aaggcctctc ctcgcgagag gctgtttttt atttgatggg ataaagatct
    31081 ttgcgcttat acggctggat ttcgcccggt ttgcgagttt tcagcaattt tagtatccag
    31141 gtgtattgtt ctggtcgcgg accaacaaaa atctcgactt cttcattcat ccgccgcgca
    31201 atcgtatgat catccgcctc taacagatca tccatcggtg ggcgcacctg aatcgtcaga
    31261 cgatgcgtct tgccatcata aatcggaaac agcggtacaa cgcgcgcacg gcacactttc
    31321 atcaaacgac caatcgcggg caacgtcgct ttataggtgg caaagaaatc cacaaattcg
    31381 ctgtgctctg ggccatgatc ctgatcgggt aaataatatc cccagtaccc ctgacgtacc
    31441 gactggatga atggtttaat accgtcattt ctcgcatgca gacgaccgcc aaagcgacga
    31501 cgcaccgtgt tccagacata atcaaaaacc gggttgccct gattatggaa catcgctgcc
    31561 attttctgcc cctgcgaggc catcagcatg gcaggaatat cgacggccca accgtgcggc
    31621 accagaaaga taactttctc gttattacgc cgcatctctt cgatgatctc cagcccttgc
    31681 cagtcaacgc gcggctgaat tttctccggc ccgcgtattg ccaactcagc catcattgcc
    31741 atcgcttgcg gcgcggtggc aaacatctca tcaacaatcg cttcgcgttc agcttcacta
    31801 cgttctggaa agcagagcga cagattgatt aacgcacgac ggcgtgagct ttttccaagt
    31861 cgtccggcaa aacgtcccag ccgtgccaga atgggatcac ggaactttgg cggcgttaaa
    31921 gcgatacccg ccatcgctgc tacgcccagc catgctcccc agtagcgcgg atggcgaaag
    31981 gatttatcaa actcaggaat gtattcgcta ttattttttt tcgtttccat gcttttccag
    32041 tttcggataa ggcaaaaatc aatctggtga tagtgtagcg gcgcaacttg ccccgcacca
    32101 aataaaaaaa gccggtactg actgcgtacc ggctgcgaat ggatgttaat taatcaaacc
    32161 gtagctgcgg cacaatctct ttggcctgcg ccaggaattc gcgacgatcg gagccggtca
    32221 gcccttcggt acgcggcagt tttgccgtca gcgggtttac ggcctgctgg tttatccata
    32281 cttcatagtg cagatgcggc ccggttgaac gtccggtatt accggaaagc gcgatacggt
    32341 cgccacgttt caccttctgt cccggtttca ccagaatctt gcgcaagtgc atataacgcg
    32401 tggtgtagct gcgaccatga cgaatagcta cataataacc tgctgcgcca ctgcgtttgg
    32461 caaccaccac ttcaccgtca cccactgaaa gcactggcgt accttgtggc atggcgaaat
    32521 caacacctct gtgtggtgca acgcgaccgg tcaccggatt agtacgacgc gggttaaagt
    32581 tagacgagat acggaactgt ttcgccgtcg ggaatcgcaa gaatcctttc gccagaccag
    32641 taccgttacg atcgtagaat ttaccatcct cagcgcggat tgcgtaataa tctttacctt
    32701 ctgaacgcaa acgtacgccc agcagctggc tttgctcacg tttaccgtca agcatttctc
    32761 gagacattaa taccgcaaat tcatcgcctt ttttcagttt gcggaaatcc atttgccact
    32821 gcatggcttt aatcactgcg ctcacttcgg cgctggttaa accggcgttt ctggcactgg
    32881 caacaaagct ccccccgacg gtacctttca gcagattgtt aacccactct ccttgctgca
    32941 tttcgctggt cattttaaaa ccgttagcgg cagtacggtc ataggttcgg gtttcacgac
    33001 gagacacttc ccaggtgagg cgctgcagtt cgccgtccgc ggttaatgtc caggagagtt
    33061 gttgaccgat tttcaggtta cgcaattctt tgtcggccgc agccagttgg gtgatatcac
    33121 ccatatcaat accatactga ttgagaatgc tgcttagcgt atcgccagtg gaaacaacat
    33181 attcatgcac gcccgcttca ccggcaattt tgtcatccag ttcgtcctgg ggaatggctt
    33241 catcttcttg tgcagcttga tcaatcggct cactggcttc aggtaagagc gaacgaattt
    33301 cgttctgttc cagctcaatg gttttgacaa ttggcgtggc atcgcggtga taaacatagg
    33361 gccgccagac agcgacggcc agagtaagaa cggtgagcga ccccaacata acgcggtgtg
    33421 gtcgcggtaa attattaaac gccagggcga cagagcgggc tatctgttgc acgtaatcac
    33481 ttcctcatta atctcctttc aggcagctcg catactggtt ggctaattga ctcaggaatt
    33541 ctgaatagct tgttttaccc agtttgatat tcgtccccag gggatccaac gttcccatac
    33601 gaacggatgt ccctcgagcg acgctctcaa cgaccgctgg cctgaactgt ggctcagcaa
    33661 aaacgcaggt tgctttttgc tcaaccaact gtgttcttat ttcatgtaaa cgctgcgcgc
    33721 caggttgaat ctcagggtta acggtaaaat gaccaagcgg tgtcagtccg aactgttttt
    33781 cgaaatagcc gtaagcatcg tgaaaaacga aataaccttt ccccttgagc ggcgcgagct
    33841 cgttaccaac ctgcgtttcg gttgaggcta attgtgcctc aaaatccttc aggttggcgt
    33901 caagtttggc tcgactttgc ggcataagtt ccactaattt tccatggatt gcaaccgctg
    33961 tagcccgcgc tatctctggg gaaagccaaa gatgcatgtt gaaatcgccg tgatggtgat
    34021 cttcgtcact tttttccgcg tggtcgtgat cctcatcatc gccgtgaata cttttcatca
    34081 gtagcggttt cacatcttca agctgcgcaa tcgttacctg cttcgcttct ggtaatttgc
    34141 ttaccggttt ttgcataaac gcttccatct ccgggccaac ccaaacgact aagtccgcgt
    34201 tctgtaagcg ttttacatcc gatggacgca gtgaataatc atgttctgaa gcgccgtcag
    34261 gaagtaaaac ctccgtttct gttaccccat cagcaatggc agaagcgatg aacccaacgg
    34321 gtttaagcga agcgacaacg gcggcatctg cggcctgtgt tgcacctccc cagagagcgg
    34381 cggataatgc tgcgaaaaga agcgtttttt tatgtaacat aatgcgacca atcatcgtaa
    34441 tgaatatgat aagtgtgata ttataacatt tcatgactac tgcaagacta aaattaacat
    34501 gacaagtctg gtttccctgg aaaatgtctc ggtttctttt ggccaacgcc gcgtcctctc
    34561 tgatgtgtcg ctggaactta aacctggaaa aattttgact ttacttgggc caaatggcgc
    34621 aggtaagtcg acactggtac gggtagtgct cgggctggta acacccgatg aaggggttat
    34681 caagcgcaac ggaaaactgc gcatcggcta tgtaccgcag aagctgtatc tcgacaccac
    34741 gttgccactg accgtaaatc gttttttacg cttacgccct ggtacacata aagaagatat
    34801 tttgcctgca ctgaaacgag tccaggccgg gcacctgatt aacgcaccga tgcaaaagct
    34861 ctctggtggc gaaacgcagc gtgtactgtt agcgcgagca ttgttaaatc gcccgcaatt
    34921 attagtgctg gatgaaccca ctcaaggcgt ggatgtaaat ggccaggtgg cgttatatga
    34981 ccttattgac caactgcgtc gtgaactgga ttgtggcgtt ttaatggttt ctcacgatct
    35041 gcatctggtg atggcaaaaa ccgatgaagt gctgtgcctg aatcaccaca tttgttgttc
    35101 cggcacaccg gaagttgttt ccctgcatcc ggagtttatt tcaatgtttg gtcctcgtgg
    35161 tgctgaacaa ctgggtatct atcgccatca tcataatcat cgtcacgatt tacagggacg
    35221 aattgttttg cgtcggggaa atgatcgctc atgattgaat tattatttcc cggttggtta
    35281 gccgggatca tgctcgcctg tgccgcgggt ccgctgggtt cgtttgtagt ctggcgtcgt
    35341 atgtcttatt tcggtgatac gctggctcat gcctcattac ttggtgtcgc gtttggtttg
    35401 ttgctggacg tgaatccatt ctatgcggtg attgccgtta cgctgctgct ggcgggcggt
    35461 ctggtatggc tggagaagcg tccacagctg gcgatcgaca cgttattagg gattatggcg
    35521 cacagtgccc tgtcgctggg cctggtggtc gttagtctga tgtctaacat tcgtgttgat
    35581 ttgatggctt acctgttcgg tgatttactg gcagtgacgc cagaagatct catctctatt
    35641 gcaattggcg tggtcatcgt ggtggctatt ttgttctggc aatggcgcaa tttgctgtcg
    35701 atgacgatta gcccggatct ggcgtttgtt gatggtgtga aattacagcg agtgaaattg
    35761 ttgttgatgc tggtgacggc attgacgatt ggtgtagcga tgaaatttgt cggcgcgttg
    35821 attattactt cgctgctgat tattcctgca gctactgcac gtcgctttgc ccgtacgccg
    35881 gaacagatgg ctggtgtcgc tgttttggtg gggatggtgg cagtaactgg cggtttaacc
    35941 ttttccgcat tttacgatac gccggcgggt ccgtcggtgg tgctatgtgc ggcactgtta
    36001 tttattatca gtatgatgaa aaagcaggcc agctaatcta tcgctgaaca catttgtcgg
    36061 atgcggcgcg agcgcctttt ccgacctacg gttcgctatc tctggtatat gcctgataag
    36121 acgcgaatag cgtcgcatca ggcacactgc caatgtcgga tgcggtgcga gcgaccaatc
    36181 cgacttacgg catttctggc ggcgttatgc caaagtgatt ccacgcccgc gtcgtcgcca
    36241 tacgcccgcg cggtgtacgc tgcaaaaagc cttgctgaat caaataaggt tccagcacat
    36301 cctcaatggt ttcacgttct tcgccaatag ccgccgccag gttatccaga cctaccggcc
    36361 caccaaagaa cttatcgatt accgccagca acaatttgcg gtccatataa tcgaaacctt
    36421 cagcatcgac attcaacata tccagcgcct gagcagcgat atctgccgag atggtgccat
    36481 cgtgcttcac ttcggcgaaa tcacgcactc gacgcagcag acggttggca atgcgcggcg
    36541 taccgcgagc gcgacgagca acttccagcg cgccgtcatc actcatctca agccccataa
    36601 agcgtgcgct gcgactgacg atatattgca gatccggcac ctgataaaac tccagacgtt
    36661 gcacaatacc aaaacggtcg cgcaacggtg atgtcagcga acctgcgcgc gtggttgcac
    36721 caatcagggt aaacggcggc aaatcaattt taatggagcg tgccgccgga ccttcaccaa
    36781 tcatgatatc cagttggtag tcttccattg ccgggtacag cacttcttca acaactggcg
    36841 ataaacggtg gatctcatca ataaacagca cgtcatgcgg ttcaaggtta gtgagcatcg
    36901 cagccaaatc gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa ttaacgccca
    36961 tttcattggc gacaatgttg gcaagcgtag ttttacccaa ccccggagga ccaaaaatca
    37021 acaaatgatc gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgtg
    37081 aacgaacctg cggctgacca acatactctt ccagtaattt agggcgaatg gcgcgatctg
    37141 ctacatcttc cggcaaagtg gtaccggcag aaatcagacg gtctgcttca atcatccttt
    37201 acctcataac gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg cgtcagggcg
    37261 agcgattttg ctcaccatgc ggcttgcttc ttgtggttta tagcccagcg ccaccagcgc
    37321 ggcaaccgct tcttgttcag catcgtcggt cgccgggcta gcaggagacg tgagtaccag
    37381 gtcggcggct ggcgtaaaga gatcgccatg caaaccttta aatcggtctt tcatttccac
    37441 aatcaagcgt tcggcggttt ttttgccaat acccggcagt ttcaccagtg cccccacttc
    37501 ttcacgctca acggcattaa cgaactgctg cgctgacatt ccggagagga tcgccagcgc
    37561 caacttcggg ccgacgccgt tggttttgat caactctttg aacaatgtgc gctcttgttt
    37621 attgttaaaa ccgtacagca gttgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat
    37681 cgcttcctga cccgcttcag ggagttcata aaaacaggtc atcggcatat gcacttcata
    37741 gcctacgccg cccacttcaa ttaacaccag cgggggttgt ttttcaatga tgatgcctct
    37801 gagtctgcct atcacatgac gctcctgcgt aatgaatcaa agataatgct gtatgataaa
    37861 aaaatgctgg atagatatcc agcgaaggat gaagaaaact tgcgaggtgt ctcgatgaac
    37921 tgaaaaatgg cacagtataa tttattctac agattatatt ggaagcaaat attttaatat
    37981 tacatattca ttgaagaaat gcgtaataaa aatatacatt gcgcctcctg aaaaaataaa
    38041 ttttttatgc tattacgtat attcgtatct atttcaatgg aatgacaacg tgaatattaa
    38101 ttaccctgct gaatatgaaa ttggagatat cgtctttaca tgtataggtg ctgccttatt
    38161 tggtcaaata acagctgcat caaattgctg gagtaatcac gtcgggatca ttatcggtca
    38221 taacggtgaa gacttcctgg ttgcagaaag ccgggttccc ctttcaacca tcaccacgct
    38281 atctcgtttt attaaacgtt cgtctaatca acgctatgct ataaagcgat tagacgctgg
    38341 gctgacagaa caacaaaaac aacgaattgt tgaacaggtt ccctcccggc tacgcaaact
    38401 ttaccacacc ggttttaaat acgaatcttc gcgccagttc tgttcaaaat ttgtttttga
    38461 tatttataaa gaagcgctat gtattccggt gggtgaaata gagacgtttg gagaattgtt
    38521 aaatagcaat ccgaatgcaa aactcacttt ctggaaattc tggttcctgg gttctattcc
    38581 gtgggagcgt aaaaccgtca cgccagccag tttgtggcat catccaggtt tggtgttgat
    38641 tcatgcggag ggagtggaaa cgcctcagcc ggaactgacc gaggcggtat aacttaacgc
    38701 agtcgccctc tcgccaggtt cagccgcgat tcgctcatct gcatcgcatt ctgactaacg
    38761 tggcagtggg tgatggcaat cgccagcgca tcggcggcat ccgcctgtgg attagcgggc
    38821 agtttcagca aggtgcggac catatgctgc acctggcttt tttcggcact accaatacct
    38881 accaccgttt gctttacctg acgtgccgcg tattcaaata ctggcaactc ctgattcacc
    38941 gccgccacaa tcgccacgcc gcgcgcctgg cccagtttca gggctgagtc agcgttcttt
    39001 gccataaaga cttgttcaat ggcgaaataa tcaggctgga actgggtgat gatttccgtc
    39061 acgcccgcat agatgagttt cagacgagac ggtaaatcat ccactttggt gcgtatgcat
    39121 ccgctaccca ggtaggacag ttgcctacct acctggcgga tgacgccgta gccggtcacg
    39181 cgcgaacccg gatcaatgcc gagaataata gccatcacgc gtctccgttt tgctgtttag
    39241 caggcctcat cagagagtcg ctgcgacctc atcagagatt tcaccgttat ggtaaacttc
    39301 ctgcacgtcg tcgcaatctt ccagcatatc gatcagacgc atcagtttcg gtgcggtttc
    39361 tgcatccata tcagctttgg tagacgggat catggaaact tccgcgctgt ctgctttcag
    39421 acctgccgct tccagagcgt cgcgcacttt acccatttct tcccatgcag tgtagacatc
    39481 aatcgcgccg tcatcatagg tcacaacgtc ttcagcacct gcttccaatg ctgcttccat
    39541 gatggtgtct tcatcgcctt tctcgaagga gatcacgcct tttttgctga acagataggc
    39601 tacggaacca tcagtaccga ggttaccgcc acatttgctg aatgcatgac gcacttcagc
    39661 aacggtacgg ttgcggttgt cagacagaca ttcaatcatg attgccgtgc cgccaggacc
    39721 gtaaccttcg tagatgatgg tttccatgtt tgcatcatca tcaccgccca caccacgtgc
    39781 aattgcgcgg ttcagtgtgt cgcgggtcat gttgttagac agtgctttat caattgctgc
    39841 acgcagacgc gggttagcgt ccggatcgcc accgcccagc ttagccgcgg ttaccagctc
    39901 acgaatgatt ttagtgaaga ttttaccgcg cttagcatcc tgcgcagctt tacgatgtct
    39961 ggtgttggcc catttactat gacctgccat aaaaatatct ccagatagcc ctgcctgttc
    40021 aggcagcgtt aattacaaac tgttcaatcg cctgccggtt gctccaggac ttagtgagcg
    40081 ccgccgcagc aggcgcatca agccacttgt aagccagatg ttcagtgaaa acgatctgcc
    40141 gctcgtgcgg aagcgcaaga cagaaccatg attccgtatt acgcgtcacg cccggcgcat
    40201 agcgatgacg taaatgtgaa aaaatttcaa actctaccgt gcgctgacag tcaattaagg
    40261 tcagttgttc agcgacaaca tcaatggtga cctcttcctt tacttcgcgc atggcagctt
    40321 gcggcgcggt ttcaccctct tccacgctgc cggttaccga ctgccagaaa tcggggtcat
    40381 cacgccgctg caacatcagc acccgtttcg tatcttgtgc gtagatgacc actaagatcg
    40441 aaacgggacg cttatacact ttatccttca cgctgcctct tcgttgactg ccttcgctca
    40501 tcccattcac atagttatct atgctcatgg gagttcactc agttgccgcc tcgatgcaac
    40561 gcgaatgatt tcgtgtattt gagtcatatc agttattctc agccttcttc acaacctgaa
    40621 tgctcagctc agccagtgca gtcgggttag caaagctcgg tgcttcagtc atcagacacg
    40681 ctgccgccgt ggttttcggg aaggcgataa cgtcacggat attgtcggtg ccggtcagca
    40741 gcatggtcag acggtcaaga ccgaatgcca gacctgcgtg cggcggagta ccgtatttca
    40801 gggcgtcgag caggaagccg aatttctcgc gctgttcctc ttcgttgata cccagaatac
    40861 caaacaccgt ctgctgcata tcaccattat ggatacgtac tgaaccaccg cccacttcgt
    40921 aaccattgat gaccatatcg taagcgttcg ccaccgcatt ttccggtgca gctttcagct
    40981 ctgcagccgt catgtctttc ggtgaggtga acggatggtg cattgccgtc aggccgcctt
    41041 caccgtcgtc ttcaaacatc gggaagtcga taacccacag cggtgcccat ttgctttcgt
    41101 cggtcagacc aaggtcttta cccactttca ggcgcagtgc gcccatcgcg tcggcaacaa
    41161 ttttcttgtt gtcggcaccg aagaaaatca tatcgccatc ttgcgcggca gtacgatcca
    41221 ggatggcttc gatgatttca gcattaagga acttcgctac cgggctgttg ataccttcca
    41281 gacctttcgc gcgttcgtta actttgatgt aagccagacc tttcgcgccg tagattttaa
    41341 cgaagttacc gtattcgtcg atctgcttac gggtcagcga tgcgccaccc ggaacacgca
    41401 gagcggcaac gcgacctttc ggatcgttcg ccggacctgc aaatactgca aactcaacag
    41461 atttcagcag atcagcaacg tcggtcagtt ccatcgggtt acgcagatcc ggtttatcag
    41521 aaccataacg gcgttcggct tccgcaaagg tcattaccgg gaaatcgccc agatccacgc
    41581 ccttcacttc cagccacaga tggcgcacca gcgcttccat cacttcacgc acttgcggcg
    41641 cggtcatgaa agaagtttcc acatcgatct gagtaaattc aggctgacga tcagcgcgca
    41701 ggtcttcgtc acggaagcat ttaacgatct gatagtagcg gtcaaagccg gacatcatca
    41761 gcagctgttt gaacaactgc ggggattgtg gcagcgcgta gaatttacct ttgtgcacac
    41821 gagaaggcac caggtagtca cgcgcgcctt ccggcgtggc tttggtcagc atcggagttt
    41881 cgatgtcgag gaagccgtgg tcatccataa aacggcgcac caggctggtg attttagcgc
    41941 gggttttcag gcgctgagcc atttccgggc gacgcaggtc gaggtagcgg tatttcagac
    42001 gcgcttcttc ggtgttgacg tggttagagt caagcggcag aacatctgcg cggttgatga
    42061 tagtcagcga ggacgccagc acttcgattt cgccggtcgc catatcgcgg ttaatatttt
    42121 tttcgtcacg cgcacgtacg gtgcccgtga cctgaatgca gaactcatta cgcagttcag
    42181 aggccagctt taacgcgtcc gcacgatccg gatcgaaaaa tacctgcacg ataccttcgc
    42241 ggtcgcgcat atcgatgaag atcaggctac caagatcacg acgacggttg acccaaccac
    42301 acagagtcac ctgctgcccc acgtgggaca aacggagctg tccacaatat tctgtacgca
    42361 tgagatatcc cttaacttag ctgccggcgg atgccccctg ctgcgcaggt gaccaagtcg
    42421 cagcgttagc tgtatgtcac aactgaatga aaaaaggcgg ctattatact ggaaattctg
    42481 ccgcaccgta agagcctggc ccgcgctgga acgcctcgtt accactttat atcgggcctg
    42541 aaatcagact ctacgccagt ttgctataaa ggtgttgccc gaactcataa aaattaacaa
    42601 aatttgtcgt tccgccatcg gctaatcgca ttaaggtgtg aggcacgatt ttgttttttc
    42661 aggagtcatt atgcttgaac ttaatgctaa aaccactgcg ctggtggtga tcgatttaca
    42721 agaaggcatc ttaccttttg ccggtggtcc acatactgcc gatgaggtag ttaatcgcgc
    42781 cgggaagctg gcggcgaaat ttcgcgccag cggtcagccc gtgtttctgg tgcgagttgg
    42841 ctggtctgcc gattacgccg aagcattaaa acagccggtt gatgccccct cccccgctaa
    42901 agtgttgccc gaaaactggt ggcaacatcc agctgcatta ggtgcaactg acagtgatat
    42961 cgaaatcatc aaacgtcagt ggggtgcgtt ttacggtacg gatctggagt tgcaattacg
    43021 ccgccgaggt atcgatacaa tagtgttatg tgggatctcg accaatatcg gtgttgaatc
    43081 caccgcccgc aatgcctggg aacttggctt taatctggtg attgccgaag acgcctgtag
    43141 tgccgccagc gccgagcaac acaataacag cattaatcat atctacccgc gcatcgcccg
    43201 tgtgcgtagc gtggaagaga tccttcacgc gttatgattt acatcggtct accgcaatgg
    43261 tcgcatccta aatgggtgcg gttggggatc accagccttg aagagtatgc ccgccacttt
    43321 aactgcgtgg agggcaacac cacgctttac gccctgccga aacccgaggt tgtcctgcgc
    43381 tggcgtgaac agaccacaga tgacttccgc ttctgtttta agtttccggc gaccatttcg
    43441 catcaggcag cattacgaca ttgcgatgat ttagtgactg aatttttgac ccgcatgtca
    43501 ccgttggctc cgcgcattgg gcaatactgg ctgcaactgc ctgccacatt cggcccacgg
    43561 gagctgcctg cgctttggca ttttctcgat tctctccctg gcgaatttaa ttatggtgtg
    43621 gaagtccgcc atccacagtt tttcgccaaa ggggaagagg aacaaacgct taatcgcggt
    43681 ttacatcagc gcggcgttaa tcgggtgatt ttagacagcc gcccggttca tgcagcacgt
    43741 ccacacagtg aagctattcg cgacgctcaa cgaaaaaaac ctaaagttcc ggtacatgct
    43801 gtactgacgg cgacaaatcc gctgatccgt tttatcggta gtgatgatat gacgcaaaac
    43861 cgggaattat ttcaggtctg gttacaaaaa ttagcgcagt ggcatcagac cactacgcct
    43921 tatctttttt tacatacgcc agatattgcc caggccccgg aactggtaca taccctgtgg
    43981 gaagacttac gtaaaacgct tccagagatc ggagcagttc cggctattcc acagcaatct
    44041 tctcttttct gaatttgcca cctatcatag acaggtgcca tcggccattt taaagggagt
    44101 ttgtatggta agcgcgctgt atgccgtttt aagtgcgttg ttattaatga agttctcttt
    44161 tgatgtcgtt cgcctgcgaa tgcagtaccg cgttgcctat ggtgacggtg gttttagcga
    44221 actgcaaagc gctattcgca ttcatggtaa cgcggtggaa tatattccca tcgcgattgt
    44281 gctgatgctg tttatggaaa tgaatggcgc agaaacctgg atggtgcata tttgcggcat
    44341 cgttttgctt gctggtcgtc tgatgcatta ttacggtttt catcaccgtc tgtttcgctg
    44401 gcgacgttcc ggcatgagcg ccacctggtg tgcgctgttg ctgatggtgc tggcgaatct
    44461 ttggtatatg ccctgggagt tggttttctc cctgcgttag cgcacaatac gccactttct
    44521 ttttcccgga tttttacgtt atgtctcacc gcgacacgct attttctgcc cctatcgcca
    44581 gactgggcga ctggaccttt gatgaacggg tagctgaagt cttcccggat atgatccagc
    44641 gttccgttcc cggctattcc aatattattt ccatgattgg tatgttagcc gagcgcttcg
    44701 ttcaacctgg tacgcaggtt tacgatctgg gttgttctct gggcgcggcg acgctctcgg
    44761 tgcgtcgcaa cattcatcat gataattgca aaattattgc catcgacaac tccccggcga
    44821 tgattgaacg ctgccgtcgt catattgacg cctataaagc ccctacgcca gtagacgtca
    44881 ttgaaggtga tattcgcgat atcgccattg aaaacgcatc gatggtggtg ctgaatttta
    44941 ccctgcaatt cctggaacct tccgagcgcc aggcattact ggataaaatt tatcaaggcc
    45001 tgaacccggg cggcgcgctg gtgctttcgg aaaaattcag tttcgaagat gccaaagttg
    45061 gtgaactgtt gttcaacatg caccacgact ttaaacgtgc caacggttac agcgaactgg
    45121 agatcagcca gaagcgcagc atgctggaaa acgtgatgct gaccgattcc gtggaaaccc
    45181 ataaagcacg cctgcataaa gccggttttg agcatagcga gctgtggttc cagtgcttta
    45241 actttggttc actggtggca ttaaaagcag aggacgctgc atgatcgact ttggtaactt
    45301 ttattctctg attgccaaaa atcatctttc acactggctc gaaacgctgc ccgcgcagat
    45361 tgctaactgg cagcgcgagc agccgcacgg gttgtttaag cagtggtcaa atgcggtgga
    45421 gtttctacct gaaattaaac cgtatcgtct tgatttattg catagcgtta cggcagaaag
    45481 cgaagagcca ctgagcgcag ggcaaattaa acgcattgaa acgctgatgc gcaacctgat
    45541 gccgtggcgc aaagggccgt tctcactgta tggcgtcaac atcgataccg aatggcgttc
    45601 cgactggaaa tgggatcgtg ttctgcccca tctttctgat ttaaccggac ggaccattct
    45661 ggatgtcggc tgcggcagcg gttatcacat gtggcgcatg attggcgcag gcgcgcacct
    45721 cgcggtgggg atcgacccta cgcaactgtt tctgtgccag tttgaagcgg tgcgtaaact
    45781 actgggtaat gatcagcgcg cgcatttgtt accgttaggt attgaacaac ttccggcact
    45841 gaaagccttt gataccgtct tttcgatggg cgtgctttat catcgtcgtt caccgctgga
    45901 gcatctctgg cagttgaaag atcaactggt caatgaaggt gaactggtgc ttgaaacgct
    45961 ggttattgat ggtgatgaga acacggtgct ggtgccgggc gatcgttacg cgcaaatgcg
    46021 taatgtctat ttcatccctt ccgcgctggc gctgaaaaac tggctgaaga agtgtggttt
    46081 tgtagatatc cgcattgcgg atgtgagcgt taccaccaca gaagagcagc gacgcaccga
    46141 atggatggtt accgaatcgc tggccgattt cctcgaccca catgatccgg gtaaaacggt
    46201 ggaaggttat ccggcaccta aacgcgcggt gctgattgcg cgcaagccgt aaaggtctgg
    46261 tattactgcc ggatgcggcg tgaacgcctt atccggccta caaaaccttg ctaattcaat
    46321 atattgcagg gactatgtag gcctgataag catagcgcat caggcagctt tatgtttgca
    46381 taacctcagc gcccgtttcc gggcgctttt cacgtcttat gcctgaacag ctggctgatc
    46441 aaacgccgtt aacttcggtg cgttacccgt atatttttcg atatacacca gcgcagagtt
    46501 accggcacag gcatttgcca gctgcgagct ggggatatcc gccgttaaca cgttcgcact
    46561 gccgttttta cacaagccat tttccagatc tggccatgca ccttcatgca cgcataccac
    46621 gccttttttg atcccgtcag tcaccaccgc gcctgtcaaa atctgaccac gtttgttcca
    46681 gacacgcacc agatcgccat tcgcaatacc aaagcgtgca gcatcttcgg tgtgaatagt
    46741 gatcggttca cgatcggcaa ccgcatattt attacggagt tccgcatagt taagctgact
    46801 gtgtaaacgg tgtgccggat gtgcggtcag aagctgtaac tgcttctcgt cggcggtacc
    46861 cttccactca tcaggcgcaa gccaagtcgg gtgtgccggg caatccttat agccaaattt
    46921 ttccagcgtt ttggagtaaa tctcaatttt gccgcttggc gtacccagcg catttttcac
    46981 cggatcggcg cggaaatcac cataacgaac atactgttcg ttcttctcgc tgcggcgcat
    47041 ttcgatcagt ttattttgct gccagaaggc attaaacatt ggcatagtga cgcgttgcgc
    47101 acgggcacct ttctgagcgg catcatagaa aaatttcagc cacgccattt catctttacc
    47161 ttcggtatag atctcttttc cgccaggttt cagtaattca gcaagatcgg caaacacgtc
    47221 aaaatcgtta cgcgcttcaa attgcggagc aacagcctgc ttcattggca caatatgctg
    47281 gttgctgtaa tcaccggtca tcgtcaagtc attgcgctca aacgatgtag tgatcggtaa
    47341 tacgatatct gcatgtttaa cagctgcggt ccagtagcat tcagaaacga cgatcatctc
    47401 cggtttctgc catgctttga tcagacgatt agtatcctgg tgatgggtaa agttaccgcc
    47461 acccgcccac cagatcattt tgatattcgg atacgtttgt tctttaccgt tatgctgata
    47521 tttgccgcca ggattttcca gcgcatcgac gatacgggca acaggaatcg ccgtcattcc
    47581 gccatcatca gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt ccggcagcac
    47641 gccgccaaca cgcgtcgggt tgccaccatt ggagtaatga taagagaaac caaaaccgcc
    47701 gcccggtgta ccaatctgcc ctaacatcgc cgccagcgtc accagcatcc agtgtttctg
    47761 ttcaccatat tgctggcgct gaattcccca gcctgccatc agcattgtgc gattcgccgc
    47821 catcagttca gcgagtttga ctatctgcgc ttccggtacg ccagtaattt ccgccgccca
    47881 ggccgcgctc ttcggagtat tatcgctctt acctgtcaga tattcttcaa actgcggata
    47941 cccggtggtg tatttttcaa ggaacacttt atcgtgtttg ccttgtgtca tcagggtatg
    48001 cgcaatccct aacatcagtg ccacgtcggt gcccatattc ggcgcgatcc aggtggcatt
    48061 atcgccaaag aattcgatgg tttcggagcg gataggatca atggcaatca ctggtttgcc
    48121 agattttttc agctgatgga agtattccag cccttgctca tcggtactgc tccaggcaat
    48181 ttttaaggta ttcagcgggt tcatccccca cagcaccaca acctggctgt tttccagaat
    48241 cagcggccag gaggtctgct gttcatacac ctctacagaa ccgaccacat gcggcatgat
    48301 cacctgtgcc gccccggttg aataatcgcc gctatgcccg gaataaccgc ccgccaggtt
    48361 catataacgt tgcagtaagg tttgcgcttt atgcaacacg ccagaagagc gccagccgta
    48421 tgatccggca aaaatagccg atggtccgtt agctttgcga atgcgatcgt gttgctcatg
    48481 aatcagtttt aatgcttgct cccagctcac ctgtacatag gtatcttcgc cacgaccttt
    48541 cgccggttgc agtggattat cgagatagct ttttctcacc atcggatgct gaatacgcgc
    48601 cgtggtgtgt acctgatccg ccgccgtaga ctgtaaggag ttcggtatgg ttttcgccag
    48661 cgcgcctgtc gaagaaacaa tcttgccgtc cttcacttct acgttcatcg ctccccaacg
    48721 tcccgcggtg aggattttac cgcccttctc ttctgcccat gcgggtagcg gtgctgccga
    48781 tgtcaccacc agcgctccag cggcaatacc gctgtgttta ataaattcac gtcttgttaa
    48841 tgtcataact tcctccctga tcaacgagga tcactgtttc tcggtaatat ctttggcgtt
    48901 gtactggaaa taccgcgtta aaatgtccag ttcgttttcg ctcatgctgg ttcgtgcccc
    48961 cattcctttg gcaatggacg cccacgcatt gacggtgtaa tggtcggcgg caataggggc
    49021 atgacaacca gcgcaatagg tatcgtcaag tttttcagcg tattgccata gcggtttacg
    49081 gtccgctaat gcgggatcgg taagcgcacc ctgtaaagac gcctgacgcc attgattgcc
    49141 gtattcgtca gcctgccatt ccccgtttac cgtgagcgcc ttgatacctt cttcacttaa
    49201 tgtggctagc gccagccgtt gacctgccgc caggtagagc gtgttttcac tgccctgcat
    49261 ttgataaccc tgcaacagaa cgatcggctg tttgccactg gcatcaacga cggtgagatc
    49321 ggtaccagga ttcacggtag ccagctcgcc tatgcgagaa gttttgaaag gataaatatg
    49381 tgcgccatta gtcactgaag tagcggcctg actttccagc tcatgcgccg cgttgtcatc
    49441 catttttatt tctggcggaa aatgggcaat gcctttatga caatcgatac aggtttcgcc
    49501 gcccttttgt gctttgttat gcattttctg cgcagattca ctttgcgagg caatatccat
    49561 ggcatcaaaa gaatggcaac tacggcacgt tgcagagtca gttgctttta attctttcca
    49621 tactgtttcg gccatttcct ggcgatgagc ttcgaactta tcgtcactgt ctattttgcc
    49681 gctaacaaat tcatgataaa tatctttaga tgcttttaat ttagcgaata aataatccat
    49741 ccctgacttt ggaatatggc aatcggcaca ttccgcacgt atccctttct ggttcgaaaa
    49801 gtggacagtt ccctgatatt cctcaaaagg tttactcatc gagtggcagg aaaggcaaaa
    49861 tgctgtatcc gacgttttat gtaaggcttt ttgcgccagc aagaacccgc caccaccaac
    49921 cacaaccgct atcagcaaaa acaataaccc aatgcgtttt ttccctcgca tagattcctc
    49981 taattaaatt ccagaac
//
LOCUS       DAZKHC020000025        47818 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZKHC020000025 DAZKHC020000000
VERSION     DAZKHC020000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 47818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.025"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            73..225
                     /gene="mokC"
                     /locus_tag="ACHOSM_RS08595"
                     /old_locus_tag="pgaptmp_001719"
     CDS             73..225
                     /gene="mokC"
                     /locus_tag="ACHOSM_RS08595"
                     /old_locus_tag="pgaptmp_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HGU4558266.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(329..1459)
                     /gene="dnaJ"
                     /locus_tag="ACHOSM_RS08600"
                     /old_locus_tag="pgaptmp_001720"
     CDS             complement(329..1459)
                     /gene="dnaJ"
                     /locus_tag="ACHOSM_RS08600"
                     /old_locus_tag="pgaptmp_001720"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGU4558267.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGVQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1548..3464)
                     /gene="dnaK"
                     /locus_tag="ACHOSM_RS08605"
                     /old_locus_tag="pgaptmp_001721"
     CDS             complement(1548..3464)
                     /gene="dnaK"
                     /locus_tag="ACHOSM_RS08605"
                     /old_locus_tag="pgaptmp_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGU4558268.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3841..4245
                     /gene="yaaI"
                     /locus_tag="ACHOSM_RS08610"
                     /old_locus_tag="pgaptmp_001722"
     CDS             3841..4245
                     /gene="yaaI"
                     /locus_tag="ACHOSM_RS08610"
                     /old_locus_tag="pgaptmp_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HGU4558269.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4271..4984
                     /gene="msyB"
                     /locus_tag="ACHOSM_RS08615"
                     /old_locus_tag="pgaptmp_001723"
     CDS             4271..4984
                     /gene="msyB"
                     /locus_tag="ACHOSM_RS08615"
                     /old_locus_tag="pgaptmp_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU4558270.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLYVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5133..5699
                     /gene="satP"
                     /locus_tag="ACHOSM_RS08620"
                     /old_locus_tag="pgaptmp_001724"
     CDS             5133..5699
                     /gene="satP"
                     /locus_tag="ACHOSM_RS08620"
                     /old_locus_tag="pgaptmp_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HGU4558271.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5734..6321)
                     /gene="mog"
                     /locus_tag="ACHOSM_RS08625"
                     /old_locus_tag="pgaptmp_001725"
     CDS             complement(5734..6321)
                     /gene="mog"
                     /locus_tag="ACHOSM_RS08625"
                     /old_locus_tag="pgaptmp_001725"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HGU4558272.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6436..7389)
                     /gene="tal"
                     /locus_tag="ACHOSM_RS08630"
                     /old_locus_tag="pgaptmp_001726"
     CDS             complement(6436..7389)
                     /gene="tal"
                     /locus_tag="ACHOSM_RS08630"
                     /old_locus_tag="pgaptmp_001726"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU4558273.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7668..9098
                     /gene="yaaJ"
                     /locus_tag="ACHOSM_RS08635"
                     /old_locus_tag="pgaptmp_001727"
     CDS             7668..9098
                     /gene="yaaJ"
                     /locus_tag="ACHOSM_RS08635"
                     /old_locus_tag="pgaptmp_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGU4558274.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9168..9944
                     /gene="yaaA"
                     /locus_tag="ACHOSM_RS08640"
                     /old_locus_tag="pgaptmp_001728"
     CDS             9168..9944
                     /gene="yaaA"
                     /locus_tag="ACHOSM_RS08640"
                     /old_locus_tag="pgaptmp_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGU4558275.1"
                     /translation="MLILISPAKTLDYQSPLTTMRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10097..10393)
                     /gene="yaaX"
                     /locus_tag="ACHOSM_RS08645"
                     /old_locus_tag="pgaptmp_001729"
     CDS             complement(10097..10393)
                     /gene="yaaX"
                     /locus_tag="ACHOSM_RS08645"
                     /old_locus_tag="pgaptmp_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HGU4558276.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10607..11893)
                     /gene="thrC"
                     /locus_tag="ACHOSM_RS08650"
                     /old_locus_tag="pgaptmp_001730"
     CDS             complement(10607..11893)
                     /gene="thrC"
                     /locus_tag="ACHOSM_RS08650"
                     /old_locus_tag="pgaptmp_001730"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGU4558277.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11894..12826)
                     /gene="thrB"
                     /locus_tag="ACHOSM_RS08655"
                     /old_locus_tag="pgaptmp_001731"
     CDS             complement(11894..12826)
                     /gene="thrB"
                     /locus_tag="ACHOSM_RS08655"
                     /old_locus_tag="pgaptmp_001731"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGU4558278.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPLAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12828..15290)
                     /gene="thrA"
                     /locus_tag="ACHOSM_RS08660"
                     /old_locus_tag="pgaptmp_001732"
     CDS             complement(12828..15290)
                     /gene="thrA"
                     /locus_tag="ACHOSM_RS08660"
                     /old_locus_tag="pgaptmp_001732"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HGU4558279.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TEQLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAVEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDELFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15316..15435)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15371..15436)
                     /gene="thrL"
                     /locus_tag="ACHOSM_RS08665"
                     /old_locus_tag="pgaptmp_001733"
     CDS             complement(15371..15436)
                     /gene="thrL"
                     /locus_tag="ACHOSM_RS08665"
                     /old_locus_tag="pgaptmp_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HGU4558280.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15650..16336)
                     /gene="yjtD"
                     /locus_tag="ACHOSM_RS08670"
                     /old_locus_tag="pgaptmp_001734"
     CDS             complement(15650..16336)
                     /gene="yjtD"
                     /locus_tag="ACHOSM_RS08670"
                     /old_locus_tag="pgaptmp_001734"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU4558281.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16736..16876)
                     /gene="yjjY"
                     /locus_tag="ACHOSM_RS08675"
                     /old_locus_tag="pgaptmp_001735"
     CDS             complement(16736..16876)
                     /gene="yjjY"
                     /locus_tag="ACHOSM_RS08675"
                     /old_locus_tag="pgaptmp_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HGU4558282.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            16972..17688
                     /gene="arcA"
                     /locus_tag="ACHOSM_RS08680"
                     /old_locus_tag="pgaptmp_001736"
     CDS             16972..17688
                     /gene="arcA"
                     /locus_tag="ACHOSM_RS08680"
                     /old_locus_tag="pgaptmp_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HGU4558283.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17748..19100)
                     /gene="creD"
                     /locus_tag="ACHOSM_RS08685"
                     /old_locus_tag="pgaptmp_001737"
     CDS             complement(17748..19100)
                     /gene="creD"
                     /locus_tag="ACHOSM_RS08685"
                     /old_locus_tag="pgaptmp_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HGU4558284.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(19158..20582)
                     /gene="creC"
                     /locus_tag="ACHOSM_RS08690"
                     /old_locus_tag="pgaptmp_001738"
     CDS             complement(19158..20582)
                     /gene="creC"
                     /locus_tag="ACHOSM_RS08690"
                     /old_locus_tag="pgaptmp_001738"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HGU4558285.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20582..21271)
                     /gene="creB"
                     /locus_tag="ACHOSM_RS08695"
                     /old_locus_tag="pgaptmp_001739"
     CDS             complement(20582..21271)
                     /gene="creB"
                     /locus_tag="ACHOSM_RS08695"
                     /old_locus_tag="pgaptmp_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HGU4558286.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21284..21757)
                     /gene="creA"
                     /locus_tag="ACHOSM_RS08700"
                     /old_locus_tag="pgaptmp_001740"
     CDS             complement(21284..21757)
                     /gene="creA"
                     /locus_tag="ACHOSM_RS08700"
                     /old_locus_tag="pgaptmp_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HGU4558287.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            21968..22837
                     /gene="robA"
                     /locus_tag="ACHOSM_RS08705"
                     /old_locus_tag="pgaptmp_001741"
     CDS             21968..22837
                     /gene="robA"
                     /locus_tag="ACHOSM_RS08705"
                     /old_locus_tag="pgaptmp_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HGU4558288.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22834..23481)
                     /gene="gpmB"
                     /locus_tag="ACHOSM_RS08710"
                     /old_locus_tag="pgaptmp_001742"
     CDS             complement(22834..23481)
                     /gene="gpmB"
                     /locus_tag="ACHOSM_RS08710"
                     /old_locus_tag="pgaptmp_001742"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HGU4558289.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23533..24045
                     /gene="yjjX"
                     /locus_tag="ACHOSM_RS08715"
                     /old_locus_tag="pgaptmp_001743"
     CDS             23533..24045
                     /gene="yjjX"
                     /locus_tag="ACHOSM_RS08715"
                     /old_locus_tag="pgaptmp_001743"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HGU4558290.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLHPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(24192..24518)
                     /gene="trpR"
                     /locus_tag="ACHOSM_RS08720"
                     /old_locus_tag="pgaptmp_001744"
     CDS             complement(24192..24518)
                     /gene="trpR"
                     /locus_tag="ACHOSM_RS08720"
                     /old_locus_tag="pgaptmp_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HGU4558291.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24608..26545)
                     /gene="sltY"
                     /locus_tag="ACHOSM_RS08725"
                     /old_locus_tag="pgaptmp_001745"
     CDS             complement(24608..26545)
                     /gene="sltY"
                     /locus_tag="ACHOSM_RS08725"
                     /old_locus_tag="pgaptmp_001745"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HGU4558292.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPDKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNLNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSLGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26756..28423
                     /gene="ettA"
                     /locus_tag="ACHOSM_RS08730"
                     /old_locus_tag="pgaptmp_001746"
     CDS             26756..28423
                     /gene="ettA"
                     /locus_tag="ACHOSM_RS08730"
                     /old_locus_tag="pgaptmp_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HGU4558293.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28584..28691)
                     /locus_tag="ACHOSM_RS08735"
                     /old_locus_tag="pgaptmp_001747"
                     /pseudo
     CDS             complement(28584..28691)
                     /locus_tag="ACHOSM_RS08735"
                     /old_locus_tag="pgaptmp_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28730..29962)
                     /gene="nadR"
                     /locus_tag="ACHOSM_RS08740"
                     /old_locus_tag="pgaptmp_001748"
     CDS             complement(28730..29962)
                     /gene="nadR"
                     /locus_tag="ACHOSM_RS08740"
                     /old_locus_tag="pgaptmp_001748"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HGU4558294.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(29983..31365)
                     /gene="radA"
                     /locus_tag="ACHOSM_RS08745"
                     /old_locus_tag="pgaptmp_001749"
     CDS             complement(29983..31365)
                     /gene="radA"
                     /locus_tag="ACHOSM_RS08745"
                     /old_locus_tag="pgaptmp_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGU4558295.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAAEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31414..32382)
                     /gene="serB"
                     /locus_tag="ACHOSM_RS08750"
                     /old_locus_tag="pgaptmp_001750"
     CDS             complement(31414..32382)
                     /gene="serB"
                     /locus_tag="ACHOSM_RS08750"
                     /old_locus_tag="pgaptmp_001750"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HGU4558296.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32488..33132
                     /gene="ytjB"
                     /locus_tag="ACHOSM_RS08755"
                     /old_locus_tag="pgaptmp_001751"
     CDS             32488..33132
                     /gene="ytjB"
                     /locus_tag="ACHOSM_RS08755"
                     /old_locus_tag="pgaptmp_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HGU4558297.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33160..34176
                     /gene="lplA"
                     /locus_tag="ACHOSM_RS08760"
                     /old_locus_tag="pgaptmp_001752"
     CDS             33160..34176
                     /gene="lplA"
                     /locus_tag="ACHOSM_RS08760"
                     /old_locus_tag="pgaptmp_001752"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HGU4558298.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34208..34471
                     /locus_tag="ACHOSM_RS08765"
                     /old_locus_tag="pgaptmp_001753"
                     /pseudo
     CDS             34208..34471
                     /locus_tag="ACHOSM_RS08765"
                     /old_locus_tag="pgaptmp_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(34632..35351)
                     /gene="deoD"
                     /locus_tag="ACHOSM_RS08770"
                     /old_locus_tag="pgaptmp_001754"
     CDS             complement(34632..35351)
                     /gene="deoD"
                     /locus_tag="ACHOSM_RS08770"
                     /old_locus_tag="pgaptmp_001754"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGU4558299.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35431..36654)
                     /gene="deoB"
                     /locus_tag="ACHOSM_RS08775"
                     /old_locus_tag="pgaptmp_001755"
     CDS             complement(35431..36654)
                     /gene="deoB"
                     /locus_tag="ACHOSM_RS08775"
                     /old_locus_tag="pgaptmp_001755"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGU4558300.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(36706..38028)
                     /gene="deoA"
                     /locus_tag="ACHOSM_RS08780"
                     /old_locus_tag="pgaptmp_001756"
     CDS             complement(36706..38028)
                     /gene="deoA"
                     /locus_tag="ACHOSM_RS08780"
                     /old_locus_tag="pgaptmp_001756"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HGU4558301.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38155..38934)
                     /gene="deoC"
                     /locus_tag="ACHOSM_RS08785"
                     /old_locus_tag="pgaptmp_001757"
     CDS             complement(38155..38934)
                     /gene="deoC"
                     /locus_tag="ACHOSM_RS08785"
                     /old_locus_tag="pgaptmp_001757"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGU4558302.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39192..40742
                     /gene="yjjI"
                     /locus_tag="ACHOSM_RS08790"
                     /old_locus_tag="pgaptmp_001758"
     CDS             39192..40742
                     /gene="yjjI"
                     /locus_tag="ACHOSM_RS08790"
                     /old_locus_tag="pgaptmp_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HGU4558303.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDESDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADADLKQVSPNLTFIYDPEITPDDLLLKVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            40714..41577
                     /gene="yjjW"
                     /locus_tag="ACHOSM_RS08795"
                     /old_locus_tag="pgaptmp_001759"
     CDS             40714..41577
                     /gene="yjjW"
                     /locus_tag="ACHOSM_RS08795"
                     /old_locus_tag="pgaptmp_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HGU4558304.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(41690..42472)
                     /gene="yjjV"
                     /locus_tag="ACHOSM_RS08800"
                     /old_locus_tag="pgaptmp_001760"
     CDS             complement(41690..42472)
                     /gene="yjjV"
                     /locus_tag="ACHOSM_RS08800"
                     /old_locus_tag="pgaptmp_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4558305.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     KPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFSVRG"
     gene            complement(42469..43542)
                     /gene="yjjU"
                     /locus_tag="ACHOSM_RS08805"
                     /old_locus_tag="pgaptmp_001761"
     CDS             complement(42469..43542)
                     /gene="yjjU"
                     /locus_tag="ACHOSM_RS08805"
                     /old_locus_tag="pgaptmp_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HGU4558306.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(43664..43825)
                     /gene="ytjA"
                     /locus_tag="ACHOSM_RS08810"
                     /old_locus_tag="pgaptmp_001762"
     CDS             complement(43664..43825)
                     /gene="ytjA"
                     /locus_tag="ACHOSM_RS08810"
                     /old_locus_tag="pgaptmp_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HGU4558307.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(43952..44557)
                     /gene="osmY"
                     /locus_tag="ACHOSM_RS08815"
                     /old_locus_tag="pgaptmp_001763"
     CDS             complement(43952..44557)
                     /gene="osmY"
                     /locus_tag="ACHOSM_RS08815"
                     /old_locus_tag="pgaptmp_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HGU4558308.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(44950..46536)
                     /gene="prfC"
                     /locus_tag="ACHOSM_RS08820"
                     /old_locus_tag="pgaptmp_001764"
     CDS             complement(44950..46536)
                     /gene="prfC"
                     /locus_tag="ACHOSM_RS08820"
                     /old_locus_tag="pgaptmp_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGU4558309.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVS
                     RLKSEYNVEAVYESVNVATARWVECVDAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            46756..47004
                     /locus_tag="ACHOSM_RS08825"
                     /old_locus_tag="pgaptmp_001765"
     CDS             46756..47004
                     /locus_tag="ACHOSM_RS08825"
                     /old_locus_tag="pgaptmp_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4558310.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            47431..47544
                     /locus_tag="ACHOSM_RS08830"
                     /old_locus_tag="pgaptmp_001766"
     CDS             47431..47544
                     /locus_tag="ACHOSM_RS08830"
                     /old_locus_tag="pgaptmp_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HGU4558311.1"
                     /translation="MNSILIITSLLIIFSIFGHALIKLGIGISNNPDKTDV"
     gene            47613..>47818
                     /gene="ydfK"
                     /locus_tag="ACHOSM_RS08835"
                     /old_locus_tag="pgaptmp_001767"
     CDS             47613..>47818
                     /gene="ydfK"
                     /locus_tag="ACHOSM_RS08835"
                     /old_locus_tag="pgaptmp_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HGU4558312.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLK"
BASE COUNT        11372 a        12899 c        11922 g        11625 t
ORIGIN      
        1 cccgaggccc cctatatgct gaacacatgt agagtgcctc ttactgaccg taaggtcaag
       61 gagaagagag caatgaagca gcataaggcg atgattgtcg ccctgatcgt catctgtatc
      121 accgccgtag tggcggcgct ggtaacgaga aaagacctct gtgaggttca catccgaact
      181 ggccagacgg aggttgctgt tttcacggct tacgaatccg agtaagagca acggcgggga
      241 gtgatccccg ccacttttca ggtgctcgca tatcttcaac gcaccctaat tttacccaag
      301 cctgcccacg ggcaggcttt tggggaggtt agcgggtcag gtcgtcaaaa aacttcttca
      361 caccatcaaa gaagctcttt gagcgcgggc tgttgtgctc accggttggg ccaccgaagc
      421 tttcttgcag ctcttgcagc agctgtttct gcttctcgtt caggcctacc ggtgtttcga
      481 cgacaacgcg gcacagcaaa tcaccctgta cgccaccgcg gacagacttg acgcctttac
      541 cgcgcatacg gaaaagctta ccggtctggg tttcgccagg cactttcagt ttgacgcgac
      601 catcaagggt cggtacttcg atttcgccac ccagcgccgc catagcgaag ttgatcggga
      661 cttcgcaata gaggttgttg ccttcacgct cgaaaatcgg gtgctgttta acctgaacct
      721 gaacgtacag atcgcctgcc ggtgcgccat gctcgcccgc ttcaccttcg cccgcaagac
      781 ggatgcggtc tccagtgtcc acccctgccg ggattttaac ggacagcgtt ttgctgcgct
      841 caacacgacc atgaccatga catttgttgc acggatcttt gatcagcgta ccgcggccct
      901 gacagtgtgg acaggtctgc tgtacagcga agaatccctg gcgcatctgc acctgaccag
      961 aaccatgaca ggtcggacag gtctgcggct gtgtacctgg ttttgcaccg ctaccgtggc
     1021 aaacgtcaca ctcttccaga gtcggaatgc ggatctcttt ggtcacgcca cgtacagctt
     1081 cttcgagggt gagctccatg ttatagcgta aatcagcacc gcgcgccgca cgttgacgac
     1141 cacgtccgcc gccaaaaata tcgccgaaaa cgtcaccaaa aatatcgctg aagtctgcgc
     1201 cgccgccaaa accgccgccg cccatgccgc cttgctcaaa cgcagcatga ccatactgat
     1261 cgtatgccgc acgtttttgc gagtcggtca gaacttcata agcttccttg atctctttaa
     1321 atttcgcctc ggcctctttg tcaccctggt tacggtccgg gtggtatttc atggccaggc
     1381 gtttgtaggc ctttttgatt tcacgctctt ccgctgtttt ggaaacgcct aaaatctcgt
     1441 aataatcttg cttagccatc ttttttaaat tgcccctaga tgaatgcacg ggcggagagg
     1501 aaattcccct tcgcccgtgt cagtataatt acccgtttat agggcgatta ttttttgtct
     1561 ttgacttctt caaattcagc gtcgacaaca tcgtcatctt tcgcgttgtt tgcagaagca
     1621 tcagcaccgg cagtctgctg ctgggcatgt tgctgctggg cgatttccat cagtttctgg
     1681 gaaacctgtg ccagctcctg cattttcgct tcgatagcgg ctttgtcttc acctttcaga
     1741 gcagtttcca gtgcagtcag tgcagactcg atagcagttt tgtcgtcagc cggcagtttg
     1801 tcgcctgctt cttcaacctg cttacgggtg ctgtgcagca gatggtcgcc ctggttgcga
     1861 gtctgtacca gctcttcaaa cttacggtca gcttcggcgt tagcttctgc gtcgcgtacc
     1921 attttctgga tttcatcttc gttcagacca gaagaagcct tgatggtgat cttctgctct
     1981 ttaccgctgt ttttatcttt cgcggaaacg tgcaggatac cgtcagcatc gatatcgaag
     2041 gtaacttcga tctgcggcat gccgcgcggt gccgggttga taccatccag gttgaactga
     2101 cccagagatt tgttatcagc cgcacgttta cgttcaccct gcagcacatg gatggttacc
     2161 gcagactggt tgtcttcagc ggtagagaac acctggctgt gcttggtcgg gatagtggtg
     2221 tttttcgcga tcagcgtcgt catcacaccg cccatggttt cgatacccag agacagcggg
     2281 gtaacgtcca gcagcagtac gtctttcacg tcaccagtca gaacaccccc ctgaacagca
     2341 gcaccgattg ctacagcttc gtccgggtta acgtctttac gcggctcttt accaaagaac
     2401 tcagcaactt tcttctgaac cattggcata cgagtctgac caccaacgag gataacgtcg
     2461 tcgatatcag atacggacag gccagcgtcc tgcagtgcaa ctttcagcgg ctcaatggaa
     2521 cggtttacca gatcttcaac caggctttcc agtttcgcac gagtcacttt gatgttcatg
     2581 tgtttcggac cggtcgcatc tgcagtgatg tacggcaggt taacgtcggt ctgctgagcg
     2641 gaagacagtt cgattttcgc tttttctgcc gcttctttca ggcgctgcat tgccagcgga
     2701 tcgttgcgca ggtcaatgcc ctgatctttc ttgaattctt caaccaggta gttgatcaga
     2761 cggctgtcga agtcttcacc acccaggtgg gtatcaccgt tggtagccag aacttcgaag
     2821 gttttttcgc cgtcaacttc gtcgatttcg ataatagaaa tatcgaaagt accaccaccc
     2881 aggtcataaa ccgcgatagt acggttgcca gtacctttgt ccagaccgta agccagcgca
     2941 gctgcggtcg gttcgttgat gatacgtttt acttccagac cagcgatacg gcctgcgtct
     3001 ttggttgcct gacgctgagc atcgttaaag tatgccggta cggtgataac agcttcagtt
     3061 accggttcac ccaagtaatc ttcagcggtt ttcttcattt ttttcagcac ttcagcagaa
     3121 atctgcggcg gtgccatttt ctggccttta acttcgaccc atgcgtcgcc gttatcagca
     3181 gcaataattt tgaacggcat gatggaaaca tcacgctgta cttcttcgtc ctggaagcgg
     3241 cggccaatca ggcgtttaat cgcaaacagg gtgttttgcg ggttcgtcac tgcctgacgt
     3301 ttagccggct gaccaaccag agtttcacca tcctgggtat aggcaataat agaaggcgtg
     3361 gtgcgatcgc cttcggcgtt ctccagcacg cgaggagtgg tgccatccat aatcgctaca
     3421 caagagttgg tagtacccag gtcgatacca attattttac ccatctaaac gtctccacta
     3481 tatattcggt catcatgtgg ttgtgagtct gtaatagggg cgaaacgtct ggtttcaact
     3541 gcccaatttc atttttttgc agactcactc actgcggttg actactaaat ggggtcgtaa
     3601 cccacgtcat caagggggag atgcaaaaat tttttgtgct tttgcgcaga aatttcgcga
     3661 tatggcagga caagacgtaa aattgacagc aattttattc atataaagaa tgaatcgcag
     3721 ccaaggcatt catcaaaaaa ttgtaataaa aagaaaagat tacgtacctg aatcttctct
     3781 ttatcagcag taaactagtg ggtattcatc cctctacctc ttcccactaa gagaatcctt
     3841 atgaaatccg tttttacgat ttccgccagc ctggcgatta gcctgatgct gtgctgcacg
     3901 gcgcaggcaa acgaccataa actcctcggc gtcattgcaa tgccgcgtaa cgaaaccaac
     3961 gatctggcgc tgaaacttcc tgtttgtcgc attgtgaaac gcatacaact ctctgccgac
     4021 catggcgatt tacagttaag cggtgcatcg gtttatttca aagccgcccg tagcgccagt
     4081 cagagtctga atattccttc agaaataaaa gaagggcaaa ccactgactg gatcaacatt
     4141 aacagtgata acgacaataa acgctgcgtc tcaaaaatca ccttttccgg tcatacggtg
     4201 aactcatcgg atatggccac gctgaaaatt atcggcgacg actaacgcca gaatatagaa
     4261 accacaaaaa atgaatgtta attacctgaa tgattcagat ctggattttc ttcagcattg
     4321 tagtgaggaa cagttggcaa atttcgcccg attgctcacc cataatgaaa aaggcaaaac
     4381 tcgcctctcc agcgtactga tgcgcaacga actgtttaaa tcgatggaag ggcatcccga
     4441 gcaacatcgc cgcaactggc agctgattgc tggagaatta cagcattttg gtggcgatag
     4501 tatcgccaac aaactgcgcg gacacggtaa attgtatcgg gccattttgc tctatgtttc
     4561 aaaacgattg aagctgaaag ccgacaaaga gatgtctacg tttgagattg agcaacagtt
     4621 actggaacaa tttctgcgta acacctggaa gaatatggac gaggaacata agcaggagtt
     4681 tctgcacgcg gtcgatgcga gggtgaatga gctggaagag ctgctgccgc tgctgatgaa
     4741 agacaaatta ttggcaaaag gcgtgtcgca tttgctttcc agccaactga cccgcatttt
     4801 acgcacccac gcagcaatga gcgtacttgg gcatggtttg ctgcgcggcg cggggctggg
     4861 aggccctgta ggtgcggcac taaatggggt taaagcggtc agcggcagcg cctatcgcgt
     4921 gacgattcca gccgtactgc aaatcgcctg cctgcgccgg atggttagcg ccactcaggt
     4981 ctgataccgg aaaccgggga aaattttcat tttcccctca aaagatcgta gacactgccc
     5041 cactggctga ttattatgcc gcgccctgaa aacactacag ttattcaggg aaattatttc
     5101 accattcatt cgatgatgat ttttgaggaa ttatgggcaa cactaagttg gctaatccgg
     5161 caccgctggg cctgatgggc ttcggcatga ccaccattct gcttaacctg cacaacgtgg
     5221 gttatttcgc tctggacggt attattcttg ccatgggcat tttctacggc ggcatcgcgc
     5281 aaatttttgc tggtctgctg gagtacaaaa aaggcaacac tttcggttta accgcattca
     5341 cctcttacgg ttctttctgg ctgacgctgg ttgcgattct gctgatgccg aaactgggtc
     5401 tgaccgatgc gccaaatgca cagttccttg gtgtctacct gggtctgtgg ggcgtattta
     5461 cgctgtttat gttcttcggc acgctgaaag gcgcacgcgt tctgcaattc gttttcttta
     5521 gcctgaccgt gctgtttgcc ctgctggcga tcggtaacat tgccggtaac gccgcaatca
     5581 tccactttgc cggctggatt gggctgatct gcggtgccag cgcaatctat ctggcgatgg
     5641 gtgaagtact gaacgagcag tttggtcgca ccgttctgcc gattggtgaa tcccactaat
     5701 tgttttgaga tccccccgct cggggggatt tttttattcg ctaacgtcgc gtcttgcact
     5761 cttcggtctg aatgctgcaa ccacttccgg tgccgtttca acgtatggcc cttccagcaa
     5821 ctgaatgcag tacggtacgc tggcaaaaat accgtgtacc acaacgttac cctcagcgtc
     5881 cttcacacct tccagcgtct ctttaataga cttcggctga ccgggtaagt taaggatcag
     5941 cgcctgtttg cgaatcacac caacctgacg cgaaaggatc gcagttggta caaaatgcag
     6001 gcttatctgg cgcatctgtt caccaaagcc aggcatctcg cggtccgcta ctgccagcgt
     6061 cgcatcgggc gttacgtcac gacgcgccgg gccagttccg cccgtggtga gcaccagatg
     6121 gcaactcatt tcatccacta gctcacacaa cgtttgctcg atgatcgcct gctcatcggg
     6181 gattaagcgg gtttccagtt caaacggcgt ggttagcgcc gatgtcagcc attcttccag
     6241 cgcagggatg cctttatcct gataaacgcc gctggatgcg cgatcagaga tggaaactaa
     6301 gccaatacgt aaagtattca tcaagatttt tccgtttaaa cagtctcgtt aaacagaatg
     6361 atacactgcg aagggagtga cagacaggct tcagaagagg tagcgtgacc gacttcccgg
     6421 tcacgctaag aatgattaca gcagatcgcc aatcattttt tccagttttt cctggtcaat
     6481 agcaaactta cggatacctt ccgccagttt atctactgcc attggatctt ggttgtgctg
     6541 ccacaggaac tcggactcag tgatacgcgc cggacgcgct ttcacttcgc cggtgtaaga
     6601 cagtttacgt tcgatagccc cttcgctctc agccagctct ttcagcagtg taggtgcgat
     6661 ggtaagacgg tcgcagcctg ccagttccag aatttcgccg atgttacgga agcttgcgcc
     6721 cataaccacg gtttcataac cgtgctcttt gtagtactgg tagatttcag atacagaaac
     6781 cacgcccgga tcttctgccg gagcgtactc tttcttatca gtattcgctt tgtaccagtc
     6841 aagaatacgg ccaacaaatg gcgagatcag gaacacacct gcttccgcac aagcacgagc
     6901 ctgagcgaag gagaacagca gggtcaggtt acagttgatg ccttcttttt ccagctgttc
     6961 tgcagcacgg ataccctgcc aggtagaagc cagtttgatc agaatacgat cgttgctaat
     7021 accagcatcg ttgtagagtt tgatcaggcg ttttgctttc gcaattgacg cttcggtgtc
     7081 ataggaaaga cgcgcatcaa cttcagttga gatacggccc ggaaccagtt tcaggatttc
     7141 cagaccaata tttactgcca gtttgtcggt cgcgtccacg atctgctgcg cgcgatcgtt
     7201 gctctgctgt ttcgcccagg cgacagcatc atcaatcaac ttacggtatt ccggaatctg
     7261 cgctgcgtta agaatgagag aagggttggt tgtggcatcc tgcggttgat acagcttcat
     7321 tgccgcgatg tccccagtgt cggccactac ggtggtgtac tgacgaaggg aggtcaattt
     7381 gtccgtcatg atagtatttc tctttaaaca gcttgttagg gggatgtaac cggtctgccc
     7441 tgatgatatc acgacgcaac gtcagcgcaa ccgccgacaa gacggttatc gccaggcgag
     7501 actgtttcgg atttctgaca ctgcgttgat atcgcccgcc atttttatac aaaacctcat
     7561 gtatgctacg cagaagttat caagtacctc gtagcgtata tacttcttaa acaattggta
     7621 acgtttacac aggaaagtca tcgcgaccgg caataagagg gatatgcatg ccagattttt
     7681 tctcctttat taacagcgtc ctttggggat cggtaatgat ttacctgctc ttcggcgcag
     7741 gttgttggtt cacttttcgc accggatttg tgcagtttcg ctacatccgc cagtttggca
     7801 aaagtcttaa aaatagcatt catccacagc caggcggttt aacctcattt cagtcattgt
     7861 gtaccagtct tgcggcgcgc gtgggtagcg gcaacctggc cggcgttgcg ctggcaatta
     7921 ccgccggtgg acctggagcc gtcttttgga tgtgggttgc cgcgtttatc ggcatggcga
     7981 cctcgtttgc cgaatgttct ctggcacaac tttataaaga acgtgacgtc aatggacagt
     8041 ttcgtggcgg accggcatgg tatatggcgc gcgggctggg gatgcgttgg atgggcgttc
     8101 tgttcgccgt ctttttgctc atcgcctatg gcataatttt cagcggagtt caggcgaacg
     8161 ccgttgcccg cgccctaagt ttttcttttg attttcctcc gctggtgaca ggcattattc
     8221 tcgctgtctt tgctctgctg gcaatcactc gcggtcttca tggcgtcgcc cggctcatgc
     8281 agggttttgt cccgttgatg gcgataatct gggtactgac cagcctggtg atttgcgtaa
     8341 tgaatatcgg gcaacttccc cacgtcattt ggtctatttt tgagagtgct tttggctggc
     8401 aggaagcggc aggcggcgcg gcgggatata ccttaagcca ggcgattact aacggttttc
     8461 agcgcagtat gttttccaat gaggcgggaa tgggttctac accaaacgca gcagcggcag
     8521 ctgcgtcctg gcctccgcat ccggcagcgc aagggattgt ccagatgatt ggcattttta
     8581 tcgacaccct ggtcatctgt acggcaagcg ccatgctgat attactggcg ggtaacggca
     8641 caacctacat gccgctggaa ggtattcagc ttatccagaa ggcgatgcgg gtgctaatgg
     8701 gttcctgggg tgctgagttt gttaccctcg tggttattct gtttgccttc agctccatcg
     8761 ttgccaacta catttatgcc gaaaacaatc tcttcttttt acgcctgaac aaccctaaag
     8821 cgatctggtg tttgcggatc tgcaccttcg caacggtcat cggcggcacc ttgctaagtc
     8881 ttccgctgat gtggcaactg gcagatatca taatggcctg catggctatt accaatttga
     8941 ccgccatttt actgctctcg cctgtggttc ataccattgc cagtgattat ctacgccagc
     9001 gtaaactcgg cgtgcgcccg gtgtttgatc cgttgcgtta tccggatatc ggtcgccagc
     9061 tttctccgga cgcgtgggat gatgtttcgc aggagtaatc acaactatcg atcaactcat
     9121 tctctttttt tgctaaagtc ggcataaatt tcctgcaagg actggatatg ctgattctta
     9181 tttcacctgc gaaaacgctt gattaccaaa gcccgttgac caccatgcgc tatacgctgc
     9241 cggagctgtt agacaattcc cagcagttga tccatgaagc gcggaaactg acgcctccgc
     9301 agattagcac gctgatgcgc atcagcgaca aactggcggg tatcaacgcc gctcgctttc
     9361 atgactggca gccagatttc acgccggaga atgcccgcca ggcgattctg gcgtttaaag
     9421 gtgatgtcta caccggcttg caggccgaaa ccttcagcga agacgatttc gattttgccc
     9481 aacagcattt gcgaatgctt tctggcttgt atggcgtact gcgcccgctc gatttaatgc
     9541 agccttatcg tctggaaatg gggatccgtc ttgagaatgc ccgagggaaa gatctgtatc
     9601 aattctgggg agatatcatc accaacaagc tgaacgaggc gctcgcagca caaggcgata
     9661 atgtggtgat taatctggcg tcagatgagt attttaaatc ggtgaagccg aagaaattga
     9721 atgccgagat tatcaagccg gtgttcctcg atgagaagaa cggcaaattt aagataatca
     9781 gcttctacgc taagaaagca cgcggtctga tgagtcgttt tattattgaa aatcggctga
     9841 ccaaaccaga gcaactgact ggttttaata gcgaaggtta cttctttgat gaagcttcct
     9901 ccagcaatgg cgaactggtg tttaaacgct acgagcagcg ttaagtttgc agtcaatgcc
     9961 ggatgcggcg tgaacgcctt atccggccta caaaagcatg cagattcaat atattgcaga
    10021 attcacgtag gcctgataag acgcgccagc gtcgcatcag gcgctgaatg ccggattgtt
    10081 acccggcatt tgtcatttag cggtgatgtt tgcctggacc atgaccgccg tgatgatcat
    10141 gaggagcttt cttatggtgg cgcggcggtg gcggcggtcc gtgtgggtgc cagcgattgc
    10201 ctcgccattc ataatgttgt ttccaccagc cgtggtcgcg ccagtgacct ccatcccagt
    10261 aatagccacg attatcacga tcgcctatct gtaattttac tgacgggact aacgtaattt
    10321 ccgcagcctg tgctgccatg ggagcgacca gaaccaggga aagtgcgagt acgatagatt
    10381 gcatcttttt cacaggttac tccttttatc attgcccgtt atgggtcgat atccggagat
    10441 tacgcgatga gaacgacggt tgttattctc cgcaatccta atctctaagt taccgcattt
    10501 attgagaatt tctccttttt ccctgtcgtt tttctccata atttcttcat aaaaaagccg
    10561 ggctgcataa aagcaaaccc ggcctgattg agataatgaa tagattttac tgatgattca
    10621 tcatcaattt acgcaacgca gcaaaatcgg cgggcagatt atgtgaaagc aagggtaaat
    10681 cagcacgttc tgccagctct tttggcagat ccaacgtttc accgagaatc gcttccacgc
    10741 tctctttaaa tttcgccgga tgcgcggtgc cgaggaacaa gccatattcg cctggattca
    10801 actggtcacg cagcgcacga tacgctacgg cagcgtgcgg ctccgaagtg tagcccagtt
    10861 cttttaactc acgcattgtc tgttgcgtgg tttcatcatc cacggctgca taacccagct
    10921 ctttcagttg ccagattttg cggcggaaca actcttccac acgcggccag ttgttcggct
    10981 gactcacgtc catcgcgttg gataacgtcg cctgagtcgc tttgggtgac cactgaccgt
    11041 cgtgcaggaa acgtggcacg gtatcgttca cgttggtcgc agcaataaaa cgtttcaccg
    11101 gcagaccgag tgacttcgcc agcagacccg ccgtcaaatc gccgaagttt ccgcttggca
    11161 ccgagacaac cagctggttg cgcgcctcct gcggcagctg cgcaacagct tcaaagtagt
    11221 agcaaatctg cgccagcaaa cggctgatgt taatcgagtt agccgagttt aaccccagcg
    11281 ccactttcag ttcttcatca tcaaacgcct gcttcaccag cgcctgacag gcatcgaaat
    11341 cgccgtcgat ggcaacagtt tcgatattgc cgcccaatgt acagaacagt ttttcttgca
    11401 gtggactgat tttgcctcgt ggatagagga taaccacttt cacattcggt aaaccgtaga
    11461 aagcatgagc cactgccgct ccggtatcac cggaggtcgc ggtcagaatg gtcactggct
    11521 tatcgcccgc aatatgggtc agcatttgtg ccataaagcg accgccgaaa tctttaaatg
    11581 ccagcgttgg cccgtggaac aattccagac aaccgacatc gctttcaaca ttggcgaccg
    11641 gagccgggaa ggcaaacgcc gcgcgcacgc gctcttccag gatttcctgc gggatttcat
    11701 caccaataaa cgccgagagg atcttcgcac tgcgggtgac aaaatccagc ttcagcatct
    11761 catcaatttc agtcaggctg aattccggca ggtcgtgcgg aaaaaacagc ccctgatttt
    11821 tgcccaaccc ctgggttacg gcttgcgcaa agctgacctg ctcgttgtga tctttcagat
    11881 tgtagagttt catttagttt tccagtactc gtgcgcccgc cgtatccagc cggcaaatat
    11941 gaacaaaacc ttcctgattt tgcaggtagt tcttacccaa ccagtcggca acgcgctggg
    12001 cggtatccgg cttgtcacat agagcgaaca aggtcgggcc ggagccggag ataccgctcg
    12061 ctaccgcgcc gatttccgcg accgcctgcc gcgcctgccg gaagccaggc agtaaccgtt
    12121 cacggtaggg ttcagcgata acatctttca tcagcttcgc ggcaagctca ggctgacggg
    12181 aatagcaggc gtgaatgaag ccagccagat gtcgcccgtg cgcaatgcaa tcctggcggc
    12241 gatactgcgc cggtaaaata gcccgggctt ctgccgtcga gactttaatc cccggatacg
    12301 ccagcaccca cagccactca tcaaaccctg gcacttgctg gctgatgatg tcgttttctt
    12361 cgatcatcaa ctgcatacca ccaagaaaac acggtgccac gttgtcgtaa tgaatgctgc
    12421 cggagatacg tccttccagc tcgcccatca aagccagcaa acgagtgtca ttaagcggct
    12481 tgccgcagtg ttcattcatc gccatcagcg ccgcgactac cgaacaggcg ctggagccta
    12541 agcccgaacc gatcggcata ttcttttcca gagtcatcgc cagtggaatt tgcttgccaa
    12601 gctcctggca aaaacgctcc cagcactgat aaacgatatt ttcccgtggt tctgacggca
    12661 gcttatcggc aaagcgtccg aggttgttga gactgaatgt ctcagccgcc tcaaccgtga
    12721 ctacatctcc gagcaatgca ccatcaacgg gtgtcaccgc cgccccgagc acatcaaacc
    12781 cgacgctcat attggcactg gaagccgggg cataaacttt aaccatgtca gactcctaac
    12841 ttccatgaga gggtacgtag cagatcggca aagacacctg cagcggtaac gtcattgccc
    12901 gcaccatatc cgcgcagcac caacggcagc ggctgataat agtggctata gaaggccagg
    12961 gcgttttcgc catttttcac tttgaacagc ggatcattac catccacttc ggcaatcttc
    13021 acgcggcaga cgccatcttc atcaatattg ccaacatagc gcaaaacttt tccttcatca
    13081 cgggccttcg ccacgcgcgc ggcaaagagc tcgtcgagct gtgacagatt cgccataaaa
    13141 gcggcaacat caccctcggc gttaaactca gcaggcagca caggttcaat ttcaatatcc
    13201 gccagctcca gttcacgtcc cgtttcacga gcgagaatca atagtttacg cgccacatcc
    13261 ataccagaaa gatcatctcg cgggtccggt tcggtataac ccatttcccg tgccagcgtg
    13321 gtcgcctcgg agaaactcat gccttcgtct aacttgccga agatataaga aagcgaacct
    13381 gaaagaatgc cggagaactt catcaattca tcaccagcat tgagcagatt ttgcaggttc
    13441 tcaataaccg gtaatccagc cccaacgtta gtgtcataga ggaatttacg ccgcgatttt
    13501 tccaccgcat gacgcaacag atggtagtaa tccatcgacg aggtgttggc ctttttgttc
    13561 ggcgtgacaa cgtggaaacc ttcgcgcaag aagtcggcat attgatccgc cactgcctgg
    13621 ctggaggtgc agtcaacaat gaccgggttc agcagatgat attctttcac gaggcgaatt
    13681 aagcgcccga gattaaacgg ctctttggct tgcgccagtt cttcctgcca gttttccaga
    13741 tttaggccat gcacattggt gagcagtgcc ttcgagttgg caacaccgca gacacgtaag
    13801 tcgatatgtt tattcttcag ccagctttgt tgacgcttca gttgctccag cagcgcaccg
    13861 ccaacgccac cgacgccaat cacaaacact tcgataacct gatcggtatt gaacagcatc
    13921 tgatgagtaa cgcgcacgcc agtggtcgca tcatcgttat ttaccacgac agagattgag
    13981 cgttcagaag atccctgagc aatggcgaca atgttgatat tggcgcgggc cagcgcggca
    14041 aagaatttcg ccgagatccc acgcaaggtg cgcataccat cacctaccac cgagataatg
    14101 gccagctgtt ccgtcaccgc cagcggctcc agtaagcctt ctttcagttc caggtagaac
    14161 tcttcctgca ttgcccgttc agcccgcaca cagtcgcttt gcggaacgca gaaactgata
    14221 ctgtattcgg aagatgattg cgtaatcagc accacggaaa tacgggcgcg tgacatcgcg
    14281 gcaaagacgc gcgccgccat gccgaccatt cctttcatcc ccgggccgga aacgctgaac
    14341 attgccatgt tattcagatt ggaaatgccc ttgaccggta attcgtcttc atcacgactg
    14401 gcaccaatga gcgtacctgg tgcttgagga tttccggtat ttttaatcag gcaagggatc
    14461 tggaactggg cgatgggggt aatggtgcgg gggtgaagaa ctttagcgcc gaagtaggaa
    14521 agctccatcg cttcctggta ggacatcgac ttcaacaacc tcgcatcggg cacctgacgc
    14581 gggtcgcagg tatagacccc gtcaacgtcc gtccaaatct cgcaacaatc ggcgcgtaaa
    14641 caggcagcca gcaccgccgc ggagtagtcg gaaccgttgc gtccaagtac caccagttcg
    14701 cctttttcat taccggcggt gaaacctgcc atcaacacca tgtgatcagc cggaatgcgg
    14761 cttgccgcaa tacggcgggt ggactcagca atatcgacgg tagattcgag gtaatgcccc
    14821 actgccagca gtttttcgac cggatcgata acggtaacgt tgtgaccacg cgcttctaac
    14881 acgccggcca taatggcgat cgacattttc tcgccacggc aaatcagcgc agcgttgatg
    14941 ctatccgggc actgccccaa caaactaatg ccatgcagga catgttttat ttgggcaaat
    15001 tcctgatcga cgaaagtttt caattgcgcc agcgggaacc ccggctgggc ggcggcgagt
    15061 cccgtcaaaa gttcggcaaa aatacgttcg gcatcgctga tattgggtaa agcatcctgg
    15121 ccgctaatgg ttttttcaat catcgccacc aggtggttgg ttattttggc gggggcagag
    15181 aggacggtgg ccacctgccc ctgcctggca ttgctttcca gaatatcggc aacccgcaga
    15241 aaacgttctg catttgccac tgatgtaccg ccgaacttca acactcgcat ggttgttacc
    15301 tcgttacctt tggtcgaaaa aaaagcccgc actgtcaggt gcgggctttt ttctgtgttt
    15361 cctgtacgcg tcagcccgca ccgttacctg tggtaatggt gatggtggtg gtaatggtgg
    15421 tgctaatgcg tttcatggat gttgtgtact ctgtaatttt tatctgtctg tgcgctatgc
    15481 ctatattggt taaagtattt agtgacctaa gtcaataaaa ttttaattta ctcacggcag
    15541 gtaaccagtt cagaagctgc tatcagacac tcttttttta atccacacag agacatattg
    15601 cccgttgcag tcagaatgaa aagctgaaaa atacttacta aggcgttttt tatttggtga
    15661 tatttttttc aatatcatgc agcaaacggt gcaacattgc cgtgtctcgt tgctctaaaa
    15721 gccccaggcg ttgttgtaac cagtcgacca gttttatgtc atctgccact gccagagtcg
    15781 tcagcaatgc catgactcgt tcgcgtaaag cttgcagttg atgttggtct gccgttgtgt
    15841 cacttttcgt cggttgttgt attaatgttg ctaattgata gcaatagacc atcactgcct
    15901 gccccagatt gagcgaagga taatccgcca ccatcggcac accagtaaga acgtcagcca
    15961 acgctaactc ttcgttagtc agcccggaat cttcgcgacc aaacaccagc gcggcatggc
    16021 tcatccatga agatttttcc tctaacagcg gcaccagttc tactggcgtg gcgtagtaat
    16081 gatatttcgc ccgactgcgc gcagtggtgg cgacagtgaa atcgacatcg tgtaacgatt
    16141 caaccagtgt cgggaaaact ttaatattat caataatatc accagatcca tgtgcgaccc
    16201 agcgagtggc tggctccaga tgtgcctgac tatcgacaat ccgcagttcg ctaaaaccca
    16261 tcgttttcat tgcccgcgcc gctgccccaa tattttctgc tctggcgggt gcgaccagaa
    16321 taatcgttat acgcatattg ccactcttct tgaccaaatg accgcgaaca gggtgatcac
    16381 tgtcaactta ttacgcggtg cgaatttaca aatccttaac gtaagtcgca gaaaaagccc
    16441 tttacttagc ttaaaaaagg ctaaactatt tcctgactgt actaacggtt gaattgttaa
    16501 aaaatgctac atatccttct gtttacttag gataatttta taaaaaataa atctcgacaa
    16561 ttggattcac catgtttatt agttgtatga tgcaactagt tggattatta aaataatgtg
    16621 acgaaagcta gcatttagat acgatgattt catcaaactg ttaacgtgct acaattgaac
    16681 ttgatatatg tcaacgaagc gtagttttat tgggtgtccg gcccctctta gcctgttatg
    16741 ttgctgttaa aatggttagg atgacagccg tttttgacac tgtcgggtcc tgagggaaag
    16801 tacccacgac caagctaatg atgttgttga cgttgatgga aagtgcatca agaacgcaat
    16861 tacgtacttt agtcatgtta cgccgatcat gttaatttgc agcatgcatc aggcaggtca
    16921 gggacttttg tacttcctgt ttcgatttag ttggcaattt aggtagcaaa catgcagacc
    16981 ccgcacattc ttatcgttga agacgagttg gtaacacgca acacgttgaa aagtattttc
    17041 gaagcggaag gctatgatgt tttcgaagcg acagatggcg cggaaatgca tcagatcctc
    17101 tctgaatatg acatcaacct ggtgatcatg gatatcaatc tgccgggtaa gaacggtctt
    17161 ctgttagcgc gtgaactgcg cgagcaggcg aatgttgcgt tgatgttcct gactggccgt
    17221 gacaacgaag tcgataaaat tctcggcctc gaaatcggtg cagatgacta catcaccaaa
    17281 ccgttcaacc cgcgtgaact gacgattcgt gcacgcaacc tgctgtcccg taccatgaat
    17341 ctgggtactg tcagcgaaga acgtcgtagc gttgaaagct acaagttcaa tggttgggaa
    17401 ctggacatca acagccgttc gttgatcggc cctgatggcg agcagtacaa gctgccgcgc
    17461 agcgagttcc gcgccatgct tcacttctgt gaaaacccag gcaaaattca gtctcgtgct
    17521 gaactgctga agaaaatgac cggccgtgag ctgaaaccac acgaccgtac tgtagacgtg
    17581 acgatccgcc gtattcgtaa acatttcgaa tctacgccgg atacgccgga aatcatcgcc
    17641 accatccacg gtgaaggtta tcgcttctgt ggtgatctgg aagattaatc ggctttacca
    17701 ccgtcaaaaa aaacggcgct ttttagcgcc gtttttattt ttcaacctta tttccagata
    17761 cgtaattgat cgtccattgt aacttcttta ctggctttca ttttcggcag tgaaaacgca
    17821 taccagtcga tattacgagt cacaaacatc atgccggcca gcgccaccac cagcacactg
    17881 gttcccaaca acagcgcgct atcggcagag ttgagcagtc cccacatcac accatccagc
    17941 aacaacagcg cgagggtaaa caacatgctg ttgcgccagc ctttcaatac cgcctgcaaa
    18001 taaataccgt tcattaacgc cccaatcaga ctggcgatta tccatgccac ggtaaaaccg
    18061 atatgttcag aaagcgccag caagagcaaa taaaacatca ccaatgaaag ccccaccagc
    18121 aaatattgca ttgggtgtaa acgttgcgcg gtgagcgttt caaaaacaaa gaacgccata
    18181 aaagtcagtg caatcagcag aatggcgtac ttagtcgccc ggtccgttaa ttggtattga
    18241 tcggctggcg tcgtcactgc gacgctaaac gcggggaagt tttcccagcc ggtatcattg
    18301 cctgaagcaa aacgctcacc gagattatta gcaaaccagc tgctttgcca ttgcgcctga
    18361 aaacctgact cgctaacttc ccgtttggct ggtagaaaat cacctaaaaa actgggatgc
    18421 ggccagttgc tggttaaggt catttcgcta ttacgcccgg caggcaccac agaaagatcg
    18481 ccggtaccgc ttaaattcag tgccatattc agctgcaggt tctgcttccg ccagtcccct
    18541 tcgggtaaag ggatatgcac gccctgcccg ccttgctcta acccggtacc gggttcaatg
    18601 gtcagtgccg ttccgttaac ttcaggcgct ttcaccacac caataccacg cgcatccccg
    18661 acgctaatca caataaaggg cttgcctaag atgatatttg gcgcgttgag ctcgctaaga
    18721 cgcgaaacat cgaaatcggc ttttagcgtt aaatcactgt gccagacctg accggtataa
    18781 atccctatct tgcgttcttc cacgttctga ttgccatcaa ccatcaatga ctcaggtaac
    18841 caaaaatgga taaaacttcg tttccgctcc acggttttat cctcttcctg caccgtataa
    18901 agctcggtca caggaatagc gataagcggc ccaacgagtt tttgcggccc gctggtactt
    18961 tggcgaatcg catcttccac atcgctacgg taatcggcac gttcgacaat cacttgccga
    19021 atcagcatta tcggaatcaa caacagcaat actgcaccaa acaaggtggt cattttccag
    19081 aacagggggg atttcaacat agtcttctcc tttgcaatgg cagcaggata cgaaggctat
    19141 gtgggaagaa tttgaagcta tgtgaagtga cggtgaagtc gaagcgaggc cagcacgcca
    19201 ccttcctgca cgttgcgcag cgtgacttcg ccgttaaaca aacgggcgac ctcactgacg
    19261 aacgccaacc ccagaccgct gcttttttgc ccatttgcac gcggcaaaga gtaaaagcgt
    19321 tcaaaaatac gtgacagcgc gtagtcagga ataccactac cggtatccag caccttaagg
    19381 gcgacgtgtt cctgatccac ttcggcgctt agcgttatgc gaccgctctc gggggtaaaa
    19441 tcgatggcgt tatccagtaa attccccagc gcctgctcca gtaacgccgg ttcagcagca
    19501 acattaacct cagtgggcat aacatgtaga gtgatgtttt tttctgccaa ctgcacggtg
    19561 cgcgcttcgc tgacgcggcg aaataatgcc gccacatcaa caacagtcag aacgacttcc
    19621 tgacgattct ccagtcttgc ctggcgtagt aacgtttcta ccaatgcctg catacgcgca
    19681 ttttgcgtca gaatgttgtc ggtaaaacga gccaccactt ccggtggcgg accttcgcgt
    19741 aaaatttccg ccgcgccacg aatcgccgcc agtgggcttt ttagctcatg ggttaacgca
    19801 taaacatact gctcaatata gtttttccct tccagcttca cgcgcatact ttccagcgcc
    19861 tgcgcaagct tacgcaactc gctactgccg agttcaggaa gaggaacggg cttattgtca
    19921 gtgacggaat cggcatagcg agtgagccgg gcaatggagc ggttgatcca ccaaaccatg
    19981 cctgcgccaa tcaccagtgc aatccccagc aaaatggcgc tggcccataa aattcgccgc
    20041 tcgctacgct taatgaccgg agccatcgcc gcgttcggtt tgcctacgct caaaacgcca
    20101 ataagccgcg agccgtccat aatcggcgcg gcaacataca tcaccgaact ttcgggatcg
    20161 gcaggatttt gcaacgtgct gcgcgcacca tactgaccac gcaacgttag ccagacgtca
    20221 ttccagcgcg aataatcctg cccaacggct ttatttgcag aatcgaacaa tactttaccc
    20281 tgcgaatcgg tcatatagac atgatattca ttgcgcactt tgttaatgcc accgatattg
    20341 gcgcgaaacg ggcgatgttg tagctgatta aacgcctgcg ccagttgccc atgcgttggg
    20401 tccccagaga gcaaatcggg acgcgccagc tccgccagca acgttgcggt gtcgatcaac
    20461 gtcccctccg ttgctcttcg cacgcccggt ttaacttctt tgacaaaaat agccagtacg
    20521 aaccaggccg ccaccgccac cagtaaaaaa tagcccagca gcaaccgcat gccgatacgc
    20581 attacaggcc cctcaggcta tatcccatgc cgcgatgagt attaatcggt gaaagatcgg
    20641 ggttgatggc gcgcagcttg gcacgcaacg ttttaatgtg ggtatcaacg gtgcgatcgt
    20701 aggtgtcctg cgcatcttcc cacacgctat ccatcagttg ctggcgggac cagacgcggc
    20761 ctggcgactt gagtaacgtc ttcagcaata aaaactcata ccgcgtcagc gttaatggcg
    20821 tgtcaaacca gctgatctgc gccgcgggtt cattcaattc aaaatgacca atacggatga
    20881 cgggagacgg cgacgagaac ttcttcaccc gacgcagtaa ggtgcgcacc ctggcgcaca
    20941 cttcgcgggg tgaaaacggt ttagccacat agtcgtcagc gccaatttcc agcccaagca
    21001 ggcgatcgac ctcttcactt cgggccgtca ggaacagtac aggtaacgcc ggatggagcg
    21061 ccagtaactg gcggcacaat tcaaagccgc tgatatcagg caaaccaacg tcgagaatca
    21121 tgacgtcggg aaccagctgg cgagctttat ccagcaccgg caagcctcgc tcaaagacct
    21181 cgacggcaaa accttcctgc tgcaacatgt agaccagcgt gtcggctatc ccttgctcat
    21241 cttccactaa ccagaccgtt tcccgttgca taaatcgcct ctgttattgc cgccacggca
    21301 tgacaggaac cgcgctaatc gcgtttttcg gcgaaccttc tacaactttg tcggagtaag
    21361 ccagataagc gagcgcgttg cgtttggcgt cataaaagcg cacgacctgt aacgacttaa
    21421 agaccaggga cgtgcgtttt ttgaatacta cctcgccctg agctttgccg tttttaatac
    21481 gatccgacag ttcaatcggc ccgacttgct gacaagaaat ggccgcatcg gaggtatctt
    21541 ccgctaggcc caatccccct ttaatacccc cggttttcgc ccggctcaca taacaggtga
    21601 catttttcac atcgggatcg tcaaaggctt ccacaacaat tttgtgatcc gggccgatca
    21661 ttttaaatac ggtgtcgacc gaaccaatct cttctgcatg agccaatggc cccagcatta
    21721 tcaggcttaa agacaggatc aaatgcttgt atttcatatt gttaccattt ttatctaata
    21781 cacgagcaat tagttcgtca cggtaaataa catattgatt aagaacaaaa tctcaattct
    21841 tttatttccg tcaagcccta aaacatactc tactaagaaa aaaacactga atgctaaaac
    21901 agcaaaaaat gctattatcc aattacctga tgtcaggtgc tcgttgttga aaggatgagg
    21961 atattttatg gatcaggccg gcattattcg cgacctttta atctggctgg aaggtcatct
    22021 ggatcagccc ctgtcgctcg acaatgtagc ggcgaaagca ggttattcca agtggcactt
    22081 acagagaatg tttaaagatg tcactggcca tgctattggc gcgtatattc gtgctcgtcg
    22141 tttgtcgaaa tcggcggtcg cactacgcct gactgcgcgt ccgattctgg acatcgcgct
    22201 gcaataccgc ttcgactctc aacagacatt tacccgcgca ttcaagaagc agtttgccca
    22261 gactcctgca ctttaccgcc gttctcctga atggagcgcc tttggtattc gcccgccgct
    22321 acgtctgggt gaattcacta tgccagagca caaatttgtc accctggaag atacgccgct
    22381 gattggtgtt acccagagct actcctgttc gctggagcaa atctctgatt tccgccatga
    22441 aatgcgttat cagttctggc acgattttct cggcaacgcg ccgaccattc cgccagtgct
    22501 ctacggcctg aatgaaacgc gtccgagtca ggataaagac gacgaacaag aagtattcta
    22561 taccaccgcg ttagcccagg atcaggcaga tggctatgta ctgacggggc atccggtgat
    22621 gctgcagggc ggcgaatatg tgatgtttac ctatgaaggt ctgggaaccg gcgtgcagga
    22681 gtttatcctg acggtatacg gaacgtgcat gccaatgctc aatctgacgc gccgtaaagg
    22741 tcaggatatt gagcgatact acccggcaga agatgccaaa gcgggagatc gcccaattaa
    22801 tctgcgctgt gaactgctga ttccgatccg tcgttaacgc tgcaactcat ctaatgcagg
    22861 ggcgtccaga tgcgagatgt cccctgcggt ttcaacaacc cagccagacg ccagccacag
    22921 gctttcctga taatccacgc gcgaaataga acagttacgc agacgtaagc ggcgctctgc
    22981 ccatgctggt aatccgagaa tcgtactcac cagacatccc agtgcaatac catggctcac
    23041 cagcagcggt cggcttccct gcggtaagtc ccggcaagat tccagtgccg cattgacgcg
    23101 atcgctgagc tcctgcattg actcgccttc aggaatacgc ccgtcaacgg tgccattgac
    23161 cagctgccga cgccagttct cttcttcttc ggtcagagaa tcgatatgtc ttttttccag
    23221 cacacccatg tttaattcac gcaggcgaga atcaaagatg atgtcacagc cgcaggcctg
    23281 ggcgatgatt tccgccgtac gccgggtgcg tcctaaatcg ctactgatga tatgcgtaat
    23341 gccaagctct ttggcacggg ttgccacctg catcgcctgt tgctcacctt tggcggtcag
    23401 agggctgtca gactggccct gaatacgtcg ctcggcgttc cactgcgttt caccgtggcg
    23461 gactaggtat acctgtaaca tgcttttttt ccgtatactg ctgtaacgtt aattatctga
    23521 gtaatgctga ttatgcacca agttgtctgt gcgaccacca atcccgctaa aattcaggcc
    23581 attctgcagg catttcatga gatcttcggc gaaggatcct gccatattgc atccgttgcc
    23641 gtcgagagcg gtgtaccgga acaacccttt ggcagtgagg aaacgcgcgc tggcgcacga
    23701 aatcgggtag caaatgcccg ccgtttacat ccagaggctg atttttgggt ggcgattgaa
    23761 gcaggcattg atggcgacag caccttcagt tgggtggtga ttgaaaacgc cagccagcgc
    23821 ggcgaggcgc gttcggctac tttgccgtta ccggcggtga ttctggaaaa agtgcgtgaa
    23881 ggcgaggcgc ttggtcccgt gatgtcgcgt tataccggta ttgatgagat tggtcgtaag
    23941 gaaggggcga tcggcgtgtt taccgccggg aaactcactc gcgccagcgt ctatcaccag
    24001 gcggtgatcc tggcgctgag tccgtttcac aatgcggtgt attaagcgcc tgatgcgacg
    24061 ctgccgcgtc ttatcatgcc taccaaacat attgatattg ctggtatttg taggacggat
    24121 aaggcgttca cgccgcatcc ggcattcggt gcatgatgcc tgatgcgcca cgtcttatca
    24181 ggcctacaaa atcaatcgct tttcagcaac acctcttcca gccactggcg cagctcaacg
    24241 ggcgcggctt tcaggctgtt agatccacgc gtaatcgtcg cgatgcccgc gccgagttca
    24301 ttttttaact cacgctgact catttcgcca cgcaacagct cttcgacaat acgcacgcga
    24361 gtccccaacg cttcgcgctc atctggcgtc agcatcaggt ttaacaacgg taaatggaga
    24421 tcgttttggt aggcattctt aagcaggtcg acaaaacgta accactcctg gtgacgctgt
    24481 tctgccatcg ctgctgaata gggtgattgt tgggccataa tatgtcgcca ttgttagcgg
    24541 gggaagcaaa atgcctcccc cggttgtact cgctaaagag tacgatagca tatcataaac
    24601 gtgcggatca gtaacgacgt ccccattccg tggcgctcat caacgtcggt ttatccccca
    24661 tgaaatagcg gtagtaagcg tcataagcca gcacgttctt cacataaccg cgcgtctcgg
    24721 agaacggaat actctcgaca aatgccacag cgtcgatacg cccggcgctg ttgccaagcc
    24781 aggttcgcac ccgtccaggt ccggcgttat aagctgctga ggagaaaata cggttattgc
    24841 caaactgctg ataaacatat tgcaggtaac tggtgccaat gttgatattc gtttccggat
    24901 ccagcaattg cccaaggcta ctataaccgg gaatagagaa catcttcacc gtatgggtcg
    24961 ctgtaccagg cataatctgc atcaggccgc tggcccctac cggtgatttc acttttggat
    25021 tccaggcgct ctcctgacgg gcaatcgcca tcgcatagct ttgcgggatc tccttcccac
    25081 tggtgtagcg tttgaaaaga tcgttgtaag ccagcgggaa tcgctcttcc agatgatccc
    25141 aaagcttccc ggcgattgtt gcctgaacgc taagatccca ccattggtta ttgaaagcat
    25201 accgagccag ttgagcctgc tctgtttttg acttgctctt caccagattg gcccactcgc
    25261 tacgcgcggt attatcgaga ttccagtaca tcaactcgcg cacgcgcgcc atctccggcc
    25321 cctgagtcag ggtgctgtca acattctgcg gcgctttatc aatcttcagc tcatactctt
    25381 caccgatgcg ttgtgctgca accatcgggt agaaaccacg ctgttgcatg agttgatgca
    25441 aaatctcttt cgcttcagct tcacgtccgc gttccagcaa taaatccgcc tgccagtaac
    25501 gccattcatc tttctctttt gcttccatcg gcagacgcgc cagccaggta ttcaggccgc
    25561 gacgatcgcc ggtgccaagc gccattcgca cacggcgttc aatcaacgac gtggattgcg
    25621 agcgcataat ggcgtcatcg cgccatttcg cctgctcgtc ggtgacatcg ttgcccatca
    25681 aacgccaggc gacgatatcg cgcagctcct gaatctgatc ttcattaagc tgctgcgcct
    25741 gggcaagcga cgggatcatc agccgtgcat tttcagcatc ctgccgcgcc acactggcaa
    25801 atgccaccgc cgccatttga cgggtaaaat cggtcgcgcc ggttgtacgc gcgaaggtca
    25861 gtaccgtatt aaggttgttc gccagtgaaa tgattgccga ggcgatagtc tggtaatcgg
    25921 caggcatctg ccctgccagc actgttacca ggcctgtgtt acccgctttc atcgccagac
    25981 ggatacgctc taaatacgcc agcggatctt gtttacctga cgcacgccag acgctaaata
    26041 acttgtcaca ggcgttaggc tggctcttgc cggttagcca cagctctttc gccccttgcc
    26101 aggcttcttc actctgcccg gtgttccatt tcgcatagta gtaattacat tgcgcttcgg
    26161 tagttccggg cttgtccggg ctaaaggcta acaagccacg ccagtcttca cgccgcgcca
    26221 gttcattgac gaaacgagat tgcagcgtgc gagcgggagg aagcgtgggg ttagcgcgaa
    26281 caaagttagt gaccgtcacc gccggttgat tcatcagatc atcggtgatc tggcggtatt
    26341 ccaggtaggg ataaagcgga taatccttca gtccaggcat catttgttcg accacatcca
    26401 tctgtcgatt atcccaggcc tgcttgattt gcgcgtaacg gctacgctgc tcatccagtg
    26461 aatcggctcg cgccacgctg ctgaccgtca gcagacagac accggcagcc aacagccgcc
    26521 aggtaacttg tttggctttt tccacaagcg catcctctaa gtgtaaatac atcaatgcag
    26581 cgtcatgccg cgtaatagat ttatgctaac cagtcattgc cgtttacgcc acgttacgga
    26641 cactttttta cttttactgc gaaggtgatc gaccaactgg gattaggcgg ggaaaaaggc
    26701 tacacttcgc ctttgaaaac tcatcatcac gataaacata gaggcgaagt ccaacgtggc
    26761 tcaattcgtt tataccatgc atcgtgtcgg caaagttgtt ccgccgaaac gtcatatttt
    26821 gaaaaacatc tctctgagtt tcttccctgg ggcaaaaatt ggtgtcctgg gtctgaacgg
    26881 cgcgggtaag tctaccctgc tgcgcattat ggcgggcatt gataaagaca tcgaaggtga
    26941 agcacgtccg cagccagaca tcaagattgg ttatctgccg caggaaccgc agctgaaccc
    27001 ggaacacacc gtgcgtgagt ccattgaaga agcggtttct gaagtggtta acgccctgaa
    27061 acgcctggat gaagtgtatg cgctgtacgc cgatccggat gccgattttg acaagctggc
    27121 cgctgaacaa ggccgtctgg aagagatcat tcaggctcac gacggtcata acctgaacgt
    27181 acagctggag cgtgcggcgg atgcgctacg tctgccggac tgggacgcga aaatcgctaa
    27241 cctctccggt ggtgagcgtc gtcgcgtagc gttgtgccgc ctgctgctgg aaaaaccaga
    27301 catgctgctg ctcgacgaac cgaccaacca cctggatgcc gaatccgtgg cctggctgga
    27361 acgcttcctg cacgacttcg agggcaccgt ggtggcgatt acccacgacc gttacttcct
    27421 cgataacgtt gcaggctgga tcctcgaact tgaccgcggt gaaggtattc cgtgggaagg
    27481 caactactcc tcctggctgg agcagaaaga tcagcgcctg gcgcaggaag cttcacaaga
    27541 agcggcgcgt cgtaagtcga tcgagaaaga gctggagtgg gtacgtcagg gaactaaagg
    27601 ccgccagtcg aaaggtaaag cacgtctggc acgctttgaa gagctgaaca gcaccgaata
    27661 tcagaaacgt aacgaaacca acgaactgtt tattccacct ggaccgcgtc tgggcgataa
    27721 agtgctggaa gtcagcaacc tgcgtaaatc ctacggtgat cgcctgctga ttgatgacct
    27781 gagcttctcg atcccgaaag gggcaatcgt cgggatcatc ggtccgaacg gcgcgggtaa
    27841 atcgaccctg ttccgtatga tctctggtca ggaacagccg gacagcggca ccatcacttt
    27901 aggtgaaacg gtgaaactgg catcggttga tcagttccgt gactcaatgg ataacagcaa
    27961 aaccgtttgg gaagaagttt ccggcgggct ggatattatg aagatcggca acaccgagat
    28021 gccaagccgc gcctacgttg gccgctttaa ctttaaaggg gttgatcagg gtaaacgcgt
    28081 tggtgaactt tccggtggtg agcgcggtcg tctgcatctg gcgaagctgc tgcaggttgg
    28141 cggcaacatg ctgctgctcg acgaaccaac caacgacctg gatatcgaaa ccctgcgcgc
    28201 gctggaaaac gccctgctgg agttcccggg ctgtgcgatg gttatctcgc acgaccgttg
    28261 gttcctcgac cgtatcgcca cgcacatcct ggactaccag gatgaaggta aagttgagtt
    28321 cttcgaaggt aactttactg agtacgaaga gtacaagaaa cgcacgctgg gcgcagacgc
    28381 actggagccg aagcgtatca agtacaagcg tattgcgaag taatgcgtaa aatgccggat
    28441 gcggcgtgaa cgccttatcc ggcctacaaa ccaagaaatt tcaatacgtt gtgaatctcc
    28501 aggcctgata agacgcggta agcgtcgcat caggcattga gcaccgaatg ccggatacgg
    28561 cgtgaacgcc ttttccggcc tacaacagcc ttagtatccg caatttcgta gcgggtcacc
    28621 cgtgctgatt cttttaaacc ttgtttaatc tcgacagcct cttccagaga ggttttcaac
    28681 tcatcaaaaa acttatttta tacctcgcct ttgagccgtt tcatcgcggt tatctctgct
    28741 cccccatcat ctcccgcacc agctccacgc agcgcaggaa acggctgtca taatcgtcct
    28801 cttcaacccg cacgaattcg atattgttct cttccagcat ctccaccagc aagttctgga
    28861 actctttgcg atccaccgaa ctgccgaggc tgcgtaaacc atccgccacc cacggcgtgt
    28921 tgttctccag caggatcacc agatcgaaac ggtattcatc aatcaacgcc tgtacgaacg
    28981 gatgctcacg cccttcgtac tttttgcaga acgcctgggt agtgacaaaa tcagtgtcaa
    29041 taaacgccac tttattggca tatttcactg caaaatcaat gtattgtgcg tggcccagcg
    29101 cgattttatc gtaatcggaa tactgcaatg cgatctcatc accgccgagg tgtgaaaaga
    29161 cataatcgcg accatattcc cacgcactgg tggtattgaa gatattggca agtttgttta
    29221 ccagggtgga tttaccgctc gactcgccac caaggatcgc cacggtacgt acaaagaacg
    29281 gcttcacttc cgtaggaata tattcccagt agcggaacgg gttttcgcgg atctgcgcac
    29341 cgctgatact cataaaggta cgtttcggat cgaccagcac cgtatcgatc cccagatgtt
    29401 ccatatactg tggcgcatcg gcttcttccg aggtgtagat caggtccggc tgaatcccct
    29461 tttcagccat aaactttttg atgccgttgc tccacacatc ccagccgtgc ggatacggct
    29521 ccatgccctc ttcgttgaaa gcatgaatgc gaatattttt ctgatattta aaagtttgta
    29581 ataaccagcg cagacgatcc ggcaccgtgg gctgctgcga catggcgctg tcttcaaaca
    29641 acgcgcggtc gcgggtatcg tcaaaaccca taatgatatg cagctcgtca acctggctac
    29701 aggcgcgctg gataaggtag atatgtccgg tatgcagcgg gtagaactta ccgaatacga
    29761 caccaatggt tttcttctgt cggggaaatt caagccccaa aaaacggtgc aacgcctcca
    29821 gcttttgcgc gctggggctt ttaattttgg cattcagtaa ctggcttaaa taccctttgg
    29881 tcataccgct ggcatcagct acctgctgta gcgtgcagcc ctgttgcttg atggcagttt
    29941 tcaggtaatc aaatgacgac atatctccct ccgtatctct cattataagt cgtcgaacac
    30001 gctaagcgcg tcggagagtt ttttaacgcc aaaaatctgc atcccttccg ccgctttttt
    30061 cggtacgtta gccgccggaa caatcgcccg gcgaaaaccg tgtttcgccg cttctgaaat
    30121 tcgttcctga ccgctgggca ccgggcggat ctcccctgcc agcccgactt caccaaacac
    30181 caccagatcc tgtggcagcg gtctgtcacg caggctggaa accatcgcca gcagtaacgc
    30241 taaatcggca ctggtttcgg ttaccttcac gccgccgacc acgttcacaa atacatcctg
    30301 atcggccatt tgcagaccac cgtgacggtg caagacagcc agcaggattg ccagacggtt
    30361 ttgttccagc cccactgcca cgcggcgcgg attcgccatc atcgagtgat cgaccagcgc
    30421 ctgaatctcc accagcaacg gacgcgttcc ttcccacacc accatcacgg agctgccgga
    30481 ggtcacttca tctccgcgac ttaagaaaat tgccgaaggg ttgctgactt cacgcagccc
    30541 ctgttcggtc atcgcgaaga cgcccagctc attcaccgcg ccgaagcggt ttttatggct
    30601 gcgcaaggtg cgaaaacggg agtcggcatc accatccaga agcaccgaac agtcgataca
    30661 gtgttccagc actttagggc cagccagcga gccatctttg gttacgtgcc caaccatgac
    30721 aatcgccaca ccgcgcgttt tggcgaagcg cgtcagataa gccgccgttt cacgcacctg
    30781 cgccacgcta ccaggcgatg attgtacatc cgccatatgc atcacctgga tcgagtcaat
    30841 taccatcagt ttcggttgct cttcttcggc aatcaggcag atctgttcga tgctggtttc
    30901 cgacaacata ttgagattgt cagtcggcag gccaaggcga tgagcgcgca ttgccacctg
    30961 ttgcagcgac tcttcgccag tgacatacag cgttttcatc tgctgggcca gtttgcacag
    31021 cgtttgcagt agcagcgtgg atttccccgc acctgggtta ccgccaatca gaatggcgct
    31081 tcctggcacc acgccgccgc ctagtacgcg gtcgaactct ttaaatccgg tggaaaaacg
    31141 cggcagctct tcaaggctga tatcggagag tttctggact tttgccaccc cggcgctacc
    31201 ggcatagccg ctgagacgct cgttacgcgc caccgttggc gacgcagcaa gacgcacctc
    31261 ggtgatggtg ttccaggcat gacaggcact gcactgcccc tgccagcgcg gataatcggc
    31321 cccgcattca ttacaaacaa aggcgcgttt tggagctttt gccacggtgt acctcgttaa
    31381 tgctgtgccg cccgcaggat ggcgggcgag caattacttc tgattcaggc tgccagagag
    31441 gatgcagaat acccccatca ggtcagcgtg acggatggtg acttccgcct tttcattcac
    31501 ttttggcttg gcatggtaag caatccccag ccctgctgct ttgatcatcg gcaggtcatt
    31561 agctccatcg ccaatcgcca cggtctgcgc cagcgggatt tcatactcct gcgcgaggcg
    31621 agtcaaagtt ttcgctttgt actgcgcgtc tacgatgtcg ccgatcacat tgccggtaaa
    31681 tttaccgtcc atgatctcca gttcattggc taccacggcg gtcaggcgca gcttgtcgcg
    31741 cagatattca gcaaagaaag taaagccgcc ggaggcaatc gccactttcc agcccagcgt
    31801 ttccagcttg agcaccagtt gcgttaagcc tggcatcagc ggcagatttt cacgcacctg
    31861 ttgcagaata ttggcgtcag cgcctttcag cgtcgccaca cggctgcgca ggctggcggt
    31921 aaaatcgagt tcgccgcgca tcgcccgttc ggttacttcc gccaccatct cgcccgttcc
    31981 ggccagtttg gcaatttcat caatacattc aatctggatg gcggtggaat ccatgtccat
    32041 caccagcaaa cccggcgtgc gcaggtgcgg gattttcccc agcggggcga catccagttg
    32101 cgcttcatgg gccaggcgcg tagcccgtgc ggtgagtgaa cctgccagac gaatcacctg
    32161 ataatcttcc acgcaccagg cggcaacaat caccatcgcc gcacccagtt tgctctggta
    32221 ttgggtcaga cgctgtttat ccagcccacg accatacagc agccagccgc tacgacctgc
    32281 gtggtaatcc agtggcatca cttcatcacc acttaatgaa agaggcagac ccggccataa
    32341 agagacatct tcaggcaggt cgcaccaggt aatgttaggc attaaggctc ctgtaaaatc
    32401 gttcgaagca gggaaaataa cgcatgaggc taccttgtat ccattgcttc tggcaacatt
    32461 aagtctcaaa ttttcaaagg gtggaagatg gctcgcacaa aactgaaatt ccggctgcat
    32521 cgggcagtga ttgtcctgtt ctgtcttgcc ttgttagtgg cactgatgca gggggcgtca
    32581 tggtttagtc aaaaccacca gcgacagcgt aatccacagc tggaagaact ggcccgcacc
    32641 ctggcgcgtc aggtgacgct gaacgttgca ccgctgatgc gtaccgactc accagatgaa
    32701 aaacgcattc aggcgatcct cgatcagtta acggatgaaa gccgcatcct cgacgcgggt
    32761 gtgtatgacg aacaaggcga tcttatcgcc cgttcaggcg aaagcgtcga agtgcgcgac
    32821 cggctggcgc tcgacggtaa aaaagcaggc ggctatttta accagcagat tgtcgagcca
    32881 attgcgggta aaaacggacc gctcggctat ctgcgcctga cactcgacac ccatacgctc
    32941 gccaccgaag cccaacaggt ggataacacc actaacattt tacgcctgat gttgctgctc
    33001 tcactggcaa tcggcgtagt gctgacccgc acgctgctac agggtaaacg cacccgctgg
    33061 cagcaatcgc ccttcctgtt aaccgccagc aaaccggtgc cggaagagga agaaagcgag
    33121 aaaaaagagt gacccattac tacaagaaag gaaatcgtta tgtccacatt acgcctgctc
    33181 atctctgact cttacgaccc gtggtttaac ctggcggtgg aagagtgtat ttttcgccaa
    33241 atgcccgcca cgcagcgcgt tctgtttctc tggcgcaatg ccgacacggt agtgattggt
    33301 cgcgcgcaga acccgtggaa agagtgtaat acccggcgga tggaagaaga taacgtccgc
    33361 ctggcgcggc gcagtagcgg tggcggcgcg gtgttccacg atctcggcaa tacctgcttt
    33421 acctttatgg ctggcaagcc ggagtacgat aaaaccatct ctacgtcgat tgtgctcaat
    33481 gcgctgaacg cgctcggcgt cagcgccgaa gcgtccggac gtaacgatct ggtggtgaaa
    33541 acagtcgaag gcgaccgcaa agtctcaggc tcggcctatc gcgaaaccaa agatcgtggc
    33601 ttccaccacg gcactttgct gctcaatgcc gaccttagcc gcctggcaaa ctatctcaat
    33661 ccggataaaa agaaactggc ggcgaaaggc attacctcag tgcgttcccg cgtgaccaac
    33721 ctcaccgagc tgctgccggg gatcacccat gagcaggttt gcgaggccat aaccaaggcc
    33781 tttttcgccc attatggcga gcgtgtagaa gcggaaatca tctccccgga caaaacgcca
    33841 gacttgccaa acttcgccga aacctttgcc cgtcagagta gctgggaatg gaacttcggt
    33901 caggctccgg cattctcgca tctgctggat gaacgcttta gctggggcgg cgtggaactg
    33961 catttcgacg ttgaaaaagg ccatatcacc cgcgcccagg tgtttaccga cagcctcaac
    34021 cccgcgccgc tggaagccct cgccgggcga ctgcaaggct gcctgtaccg cgcggatatg
    34081 ctgcaacaag agtgcgaagc gctgttggtt gacttcccgg accaggaaaa agagctacgg
    34141 gagttgtcga cgtggatagc gggggcggta aggtaactcg ctgattaaaa taccaaagga
    34201 agggaatatg atcgatctgg aaaaccagga gcgggagatt atcaatttaa tgttaagcca
    34261 gcgtatttcc tggctagccg ccgtgcgtat caggcataaa ttatcgcttg ctgaagtaag
    34321 taaaatgtta ggtatctcga ttaattctct aaaatagatc gaaaagacgg aacggtttag
    34381 ctcaaacatc aaaagcaaaa tggcagaaat ttatggttgt ccaccagagt tgctgatatg
    34441 cccttcctgg atgacggctg aacataaatg aatagattcg tgaatagata cttgaataga
    34501 ttttattttc tatccatatg aaaaataata gtaaatctat aaatcagttg ctattgatga
    34561 atagatacta aaaagccgga gcagtctccc actccggctt cacacgacaa tcgcctttca
    34621 gcgaaacaca attactcttt atcgcccagc agaacggatt ccagtgcgat tttgatcatg
    34681 tcgttgaagg tggtctgacg ctcagcggca gtggtctgct cgtgagtgcg gatgtggtca
    34741 gacacggtac agatggtcag ggctttcgcg ccaaattctg cagcgacgcc gtagatacca
    34801 gccgcttcca tttccacgcc gaggatgccg tatttttcca tcacgtcgaa catttcgccg
    34861 tccggagagt agaacaggtc agcggagaac aggttaccaa cgcgagcatc aatgcccagt
    34921 gctttagccg cgtctaccgc gttacgcacc atgtcaaagt cagcgatagc ggcaaagtca
    34981 tgatctttaa aacggatgcg gttaactttg gaatcggtgc aggcacccat accgataacg
    35041 acgtcgcgca gttttacgtg cggcagaact gcgccacagg aacccacgcg gataattttc
    35101 ttcacgccga aatcggtgat cagttctttg gtgtagatgg agcaggacgg gatacccata
    35161 ccgtgaccca ttacggaaat tttgcggcct ttgtaagtac cggtgaagcc cagcataccg
    35221 cgaacgttgt tcacttcacg ggcatcttca aggaaagttt cagcaatata cttcgcacgc
    35281 agcgggtcgc ctggcatcaa aactacgtca gcgaaatcgc ccatttctgc attaatgtgt
    35341 ggggtagcca ttgttttatc cttatcaaca tgttttcacc ctctccctgt gggagagggc
    35401 cggggtgagg gcatcaagcc gcacccatta tcagaacatg gctttgccat attccatatc
    35461 agaagtacca aaatattttg ccagagtctg gccgatatcc gcgaaggttt cacggtgacc
    35521 cagtgaaccc ggttttactt tcgggccgta aaccagtacc ggaatgtgtt cacgcgtgtg
    35581 gtcagtaccg gtccaggtcg gatcacaacc gtggtcagca gtgaggatca ggatgtcgtc
    35641 atcgcgcagc aaagacatca gctccggcag acggcggtcg aacagctcca gacccgcagc
    35701 ataaccggcg acgtcgcgac ggtggcccca ggaagagtcg aagtcaacga agttggtgaa
    35761 gacgatggtg ttatcacccg cttctttcat ctctttgatg gtggcgtcaa atagcgcgtc
    35821 caggccagtt gctttcactt tcttggtgat accgcagttg gcgtagatgt ctgcaatttt
    35881 accgacagaa accacctgac cgtgtttttc atcaaccagt ttctgcagca cggtcggtgc
    35941 tggcggctca acagccaggt cgtgacggtt accggtacgc tggaagttac cggctttgtc
    36001 accgataaac ggacgagcga taacacgacc gatgttgtag ccgccgttgg tcagctcttc
    36061 acgggcgatt tcgcacagtt catagagttt atccagaccg aaagtttctt catggcaggc
    36121 aatctggaac acggagtcag cggaggtata gaaaatcggc ttgccggttt tcatgtgctc
    36181 ttcgcccagt tgatccagaa tgaccgtacc ggaagagtgg cagttaccga ggtaacccgg
    36241 cagattagcg cgttcgacca gtttatccag cagctcttgc gggaagctgt tttcgtgatc
    36301 ggagaaatat ccccactcaa acagaaccgg gacaccggcg atttcccagt gaccagacgg
    36361 ggtatcttta ccggatgaca tttcgtgcgc ccatgcgtac gcgccgataa cttcagcgtt
    36421 gccgtccatt cccgccggaa tgaaaccggt agaaccttcg tgtgctttcg ccagccccag
    36481 acgggtcaga tttggcagat tgagcgggcc tttacgaccg ttatcagctt cgcctttggc
    36541 acaagcttct gcgatatgac ccagggtgtc agccccgacg tcaccaaagc gttctgcatc
    36601 ttctgtagcg ccgatgccga atgagtccag caccataata aatgcacgtt tcatatgttc
    36661 tccgtactca gtgcttcaaa tttaaatgat cagatcagta taccgttatt cgctgatacg
    36721 gcgatagaca gttggtgtgc tttccggtgc tttatcggca agtttaattg ccgctttcac
    36781 cgctttcgcc gcttcctgcc agctgttttc gtctttcgcg tggataaccg ccagcggacg
    36841 ctgaccgtct acctggtcgc ccaaacgcgc catatcagta aagccgacgc tgtaatcaat
    36901 ggtgtcagat gcctgacgac gaccgccgcc cattgcaacc actgccatcc ccagcgcgcg
    36961 ggtatccatt tcactgacaa aaccttcggt atcagcatag actgctttcg tcagcatcgc
    37021 tgtcggcaga tacttcgcgt agttctcaac gaagtcggtc gggccttttt gtgccgctac
    37081 catacgacca aagacttctg ccgctttacc gttgtccagc accgcctgca atttcgcgcg
    37141 cgcttcggcg tcatctttcg ccagtttgcc ggagatcagc atctccacgc acagcgccat
    37201 cgtgacatca aacagacgcg ggttacgata ttcacccgtc aggaactgca ccgcttcacg
    37261 aacttcaacc gcgttacctg cactggaggc cagtacctga ttcatgtcgg tgagcagcgc
    37321 ggtggtgcgc acgccagcgc cgttagccac gccaacaatc gcttcggcaa gggcttcaga
    37381 gagttcgtag gtcggcataa acgcgccgct acccactttc acgtccatca ccagcgcatc
    37441 cagaccttcc gccagtttct tcgccaggat cgaggcagtg atcagcggga tggagtccac
    37501 ggttgcggta atatcgcggg tcgcgtagaa acgtttatct gccggagcca gtgagctggt
    37561 ctggccgata atcgccacgc cgacgtcttt aataatttcg cggaaacggt tgtcatccgg
    37621 gaaaatgtcg aagccaggga tggattccag tttgtcgagc gtaccgccag tatgaccgag
    37681 gccgcgacca gagatcatcg gaatatagcc gccgcaggct gcgaccatcg gccccaacat
    37741 cagcgaagtc acatcgccga cgccgccggt ggagtgttta tcaacaatcg ggccattcag
    37801 atgcaggctt ttccagtcga gaacggttcc tgaatctcgc atcgccatgg tcagcgagac
    37861 acgctcaggc attgtcatat cgtggaagaa aatggtcatc gcgagggcgg caatctgccc
    37921 ttcggagata gtgttgtcgc gaataccgtt gataaagaaa cgaatttctt catcgcttag
    37981 cgcatgacca tcacgttttt tacgaataat ttcttgtgcg agaaacaagg taacctcccg
    38041 aggaaaagag tatgaggcgg cttttgccgg atgcgtctac gccttatccg gcctacgggt
    38101 agatacgtct cgtaggcctg ataagcgcag cgcatcaggc gtaaagcatc ttacttagta
    38161 gctgctggcg ctcttaccgt cgccgtggcc cagcgctttc agcaggcttg ccagcaggct
    38221 ggaagcacca aagcggtagt gacgcgcatc agcccagtca gcaccgaaca gttcatctgc
    38281 gatggcgaga tatttctgcg catcttccgc agtacgcacg ccgcccgccg gtttgaaacc
    38341 aacggttttt tctacgccca tatcacggat cacttccatc atgatgcgcg cgctttccgg
    38401 cgtcgcgttc acagccactt taccggtaga ggttttgatg aagtccgcac ccgctttgat
    38461 ggagatttca gacgctttac ggatcagcgc ttcgtctttc agttcgccgg tttcgatgat
    38521 cactttcagc agtacattcg ctgccgcgca agcctcttta caggctttca ccaggtcaaa
    38581 accaacctgc tcgttacccg ccatcagcgc gcggtacggg aacacaacgt caacttcatc
    38641 agcaccgtag gcgattgccg cacgggtttc tgccagcgcg atttcgatgt catcgttacc
    38701 gtgtgggaag ttggttaccg tagcgattcg gatttccggg gtgccctgct ctttcagcgt
    38761 tttgcgagca atcgggataa agcgaggata gatacagata gcggcggtat tgccgaccgg
    38821 agttttggcc tgatgacaca gagcaattac tttctcgtcg gtgtcgtcgt cattcagggt
    38881 ggtcaggtcc atcaatttca gtgcacgcag gctgcttgct ttcagatcag tcatttcatt
    38941 ctcctggctt gtcgccaata aaattcacct tgcgagtttg ttagtattct aacatctact
    39001 ccgcaacaca cttcgatata catcacaatt aaggaaatct acttacaagt ttgcatcact
    39061 gtaatgcgat ctggttcaaa taattcactt tcaaatgaat gcgtcagtgg tggcaaacgc
    39121 atcaggatca aagtgaacat cacgaaactt cttacaatgg cgcatcacct tcggcataaa
    39181 aggaaaagaa gatgcccact tctcatgaaa atgcactgca acaacgttgc cagcaaattg
    39241 tcaccagccc agtgcttagc ccggagcaga agcgccattt tctggctctg gaagcagaaa
    39301 acaatctgcc ttatccgcag cttcctgccg aagcccgccg cgcgctggat gagggtgtaa
    39361 tctgcgatat gtttgaaggt catgcgccgt acaaaccgcg ctatgtctta cccgattacg
    39421 cccgttttct ggcgaacggt tccgaatggc tggagctgga aggcgcgaaa gatcttgatg
    39481 acgcactctc tctgctgacc attctttacc accacgtacc gtcggtcaca tcgatgccgg
    39541 tctacctggg gcaactggat gcgttgttgc aaccatatgt tagaattcta acacaagacg
    39601 agagcgatgt tcgaataaaa cgtttctggc gttacctcga cagaaccctg ccagacgcct
    39661 ttatgcacgc caatatcggc ccgtctgatt cgcccattac ccgcgcgatc ttacgtgcag
    39721 acgcagattt gaagcaggtt tcaccgaacc tgacctttat ctacgatcct gaaatcaccc
    39781 ctgatgacct gctgctgaaa gtggcgaaga acatctgtga atgtagcaaa ccccacatcg
    39841 ccaacggtcc ggtgcatgat aaaattttca caaaaggggg ctacgggatt gtgagctgtt
    39901 acaactcact gccgctggcg ggtggtggca gcacgctggt acgccttaac ctgaaagcca
    39961 ttgccgagcg cagcgaatcg ctggatgact tctttacgcg cactctaccg cactactgcc
    40021 agcagcagat cgccatcatc gatgcgcggt gtgaattcct ctatcaacaa tcacacttct
    40081 ttgagaatag cttcctggtg aaagaagggc tgattaaccc tgaacgtttt gtgccaatgt
    40141 ttggcatgta tgggctggcg gaagcggtta acttgctgtg tgagaaagaa gggattgccg
    40201 cgcgctacgg taaagaagcc gccgcaaatg aagtgggtta tcgcatcagc gcgcaactgg
    40261 cggagtttgt cgccaatacc cccgtgaaat atggctggca aaaacgcgcc atgttacacg
    40321 cacagtcggg gatcagttcc gatatcggca ccacgccggg cgcgcgtttg ccgtatggcg
    40381 atgagccaga tccgatcacc catctgcaaa ctgtcgcccc gcatcatgct tattattatt
    40441 ccggcatcag cgacattctg acgctcgacg aaaccatcaa acgtaacccg caggcactgg
    40501 tacagctttg cctcggtgcc tttaaagccg gaatgcgtga atttaccgcc aatgtcagcg
    40561 gtaacgatct ggttcgcgtt accggttata tggtgcgttt gtcggactta gaaaaatatc
    40621 gcgccgaagg ttcacgcacc aacaccacct ggctgggcga agaagccgca cgcaacactc
    40681 gtattctgga acgccagccg cgcgtgataa gccatgaaca gcagatgcgc tttagtcagt
    40741 aagattatcc ccttctcctg cgttgacggg ccaggcagtc gtctggctct gtttttgcag
    40801 ggctgcaatc tgcgctgcaa aaactgtcac aatccgtgga cgatgggacg ctgcaatgat
    40861 tgcggagagt gcgtgccaca gtgtccgcat caggcgttgc agattgttga cggcaaagtg
    40921 ctgtggaacg ctgtggtctg cgagcagtgt gatacctgcc tgaagatgtg tccgcaacat
    40981 gccacgccaa tggcgcaatc catgagcgtg gacgaagtgc ttagccatgt ccgcaaagcg
    41041 gtgctgttta ttgaagggat aacggtaagc ggcggcgaag ccacgaccca actgccgttt
    41101 gtagtggcgc tgtttactgc tatcaaaaac gatccgcaac tgcgccacct cacctgcctg
    41161 gtggacagta acggtatgtt gagcgaaacc ggctgggaaa aattactccc ggtgtgcgac
    41221 ggcgcaatgc tcgatctcaa agcgtggggg agcgaatgtc atcagcaatt aaccggacgc
    41281 gataatcagc agattaagcg cagcatctgt ttgctggcag agcgcggtaa gctggcggaa
    41341 ctgcgtttgc tggtaattcc agaccaggtg gattatttgc atcacatcga tgagctggcg
    41401 acgtttatca agagacttgg cgatgttccg gttcgcctga atgcgtttca tgcccacggc
    41461 gtgtatggcg aggcgcaaag ctgggcgagc gccacgccgg aagacgttga gccgttggct
    41521 gatgcgttaa aggtgcgcgg ggtgagccgg ttgatatttc cggcgctcta tttgtgacat
    41581 gccggatacg gcctacggtt cggcacaaac ctgtaggcat gataagacgc gttaagcgtc
    41641 gcatcaggca ttatgcgccg actgccggat gctgcctacg aagggtaaac tacccacgaa
    41701 cgctaaacac cgcatacgta ttattaagca acacttcggc aatctcatct tctggttccg
    41761 gacgtaactc gcaaagcacg tcaaacaccc gcgcagcctg ttccgggcgg ttaggcttcc
    41821 cctgaaagcc gttgagcggc atatccggcg cgtcggtttc cagcaacaac gatgccagcg
    41881 gtaattttgc gatgacatcg cgggttttac tggcgcgtgg ataggtgata gtaccgccta
    41941 cgccgatttt gtagcccagc tgtacaaacc gctcggcctg ttgcaggctg ccggaaaaac
    42001 cgtgaaccac gccagtgcgc gataaatcgt ggcgtttaag atgcatcgcc agtttgtcgt
    42061 gtgtgcgccg tgaatgcagg atcaccggca gatcgtagcg tttcgccagt ttcagctgtt
    42121 cgtcgagtaa ccactgctgc ctctcaagtt gcggatcgtc gccgaagaga tccagaccga
    42181 cctcccccac tgccaccacc ttcgccggac gcctttccag cgcctgctgt agctgctcaa
    42241 gagacacatc gctatgtttt tccaacatac ctggatgcaa gcccaatgcg gcatacagcg
    42301 gttgatattt ttccgctaac gcctgcacac gggcaaaatt ttccgcctct gtcgccggaa
    42361 caatgatctt gcctacgccc gcttgtgccg cgcgttgcag gctggcctct tcatcgccac
    42421 taaacggcgg gaaatcaaaa tggcagtggg tgtcgataaa acggcaaatc aagccagatc
    42481 ctcattgtta aatgtcgggt cgttcgcctg cggagcgtca atcacttcgg caaccagcgt
    42541 atcgttggcg actggcgcag gcggaataac gatcgattcc ggcgtcacca ctggcaccag
    42601 atggcgggta agcggcgctt tttcagttaa tagcttgcca accgtggcga ggaaataacg
    42661 accgcataaa cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc
    42721 gatactatgt aatggcttcg gcggataaat ttcgaatatc cgcagcttgc ccggtggttt
    42781 ctcaataaat tgctgaatgt cacgatagct ggtttcatga tgctgcacca gattgaccag
    42841 cggctgcagg ctactgtcac ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta
    42901 gtacatttgt gacggcacag tgcgaatgac gaccaacgtt ttagcgccct gccttgccgc
    42961 ctctttaacc ggaatcgcat cactgatccc gccatccagg tagttaatgc cttccagcga
    43021 cactccgcta cgataaaagc caggtatcgc actggaggcg cgaatcacat ccagccagtt
    43081 ttgtttggtt ggtaaaaagt aattaggcgc gtagtcatcc tgacgacagg cgcacatata
    43141 aaacgatttg ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg gcatctggct
    43201 tgcggtggcc tccaccagcc agtcgagatc gataagattt cctccacgga caaagcgcaa
    43261 tggatcgaaa aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg
    43321 ctgattgcag ataaacgccg agaggttctg cgccccggca gatgtgccga gataaagatc
    43381 gaaaggatta aactgcgcgc gcataaactc atccagcacg ccagccgtga aaattccacg
    43441 ctgtccgccg ccttcgcaca ccagagctat tcgtccaggc tggaacggcc ttagcgacaa
    43501 cggcgcaata ttaccaagcg ttacaggtat tcgctgcccc acctctgcct tcctgttttt
    43561 gttgtgaaag aatgacaaag taacgcaatt tattattcgc taaaaccgca aaagccagtc
    43621 cgctggactg gctttggata aaattttctt aatggtctga aatctagggg cgttttcggc
    43681 ccatgaacaa actcaccagg aacagaataa tcccgacgac aaagacaatt ttagctgcgc
    43741 ctgcagcggt accggccaga ccaccaaacc caagtgcggc ggcgattaac gcgataacca
    43801 gaaatatgat gccccaacga aacataagcg tctcctttac catagttaat gtcaccgctt
    43861 gatatgagcg tgaaaatcac tcaatgcgat atttttagtg tggtgcacat cgcgcctccc
    43921 gacaaagttc gggaggacga attacgacga attacttagt tttcagatca tttttaacgc
    43981 ttttcacacc atctaccgct ttggcgatac tttcagcacg gtcgctctgt gcctgggaat
    44041 cgacggtacc ggagagctga accacgccgt cggtggtttc aactttcaca tgacgggaag
    44101 ggacgatatc gtccgccagc agtttggctt tgatttcact ggtggtggcg gtgtcacccg
    44161 cgtagccctt cacagagcct tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg
    44221 tcaccccttc aacgcctttc gccactttca ctgcctcttc ggcctgagcc tggctttcaa
    44281 cgaaaccgct cagggtcacg actttttgat cggttttgac agagatatcg gtgctcttga
    44341 tattgtcatg atccaccagc gccgccttca ctttcgcggt gatggcgcta tcatccatga
    44401 aattaccgac tttattcata gagctatcga ctttttgccc tgcgctttca ttggtagtct
    44461 gtgcgttgtt ttccgcgtag gcagagccgg tcgcgacggc agaggtcaac attacagcca
    44521 gcagagtttt cgaaatcttc agtcttgtca tagtcatcga tttattcctg tatgtttgct
    44581 cgtaatttga gcctggcaac acgaggttgc attgctgaat agggagagac ttcaccctct
    44641 acagaagtca atggtcgcca tcacaaaagc gatgggtgat gaataacgac cattacagcc
    44701 tctgaatcag ttattaatat cggtagaatg acaatcgacg gctttagata ctgatatcta
    44761 cgcattgaac ggtatttaac gccgtcagaa atgtcattac tttgttaaat atagatcaca
    44821 attttgaaac cgctcgggat atcagcgaaa acataagcaa aagtgaatgt tttaagaaca
    44881 ttccgtaagc ggctaataag gaagggaaat tgacagggcg cagcggctac cgcgccctgg
    44941 aggcaagaat taatgctcgc gggtctggtg gaactgaacg tccggataac gttcctgcgc
    45001 caggcgcagg ttgaccatgc tggtagcgat gtaagcgagg ttatcgccgc catcaagcgc
    45061 cagttggctt tcgttcttac gcttgaactc ttcgaatttc ttcgcgtcca cacattctac
    45121 ccagcgggca gtggcaacgt tgactgattc atatactgct tcaacgttgt attcgctctt
    45181 caggcgcgat accaccacat caaactgcag cacaccaaca gcaccaacga tcaaatcgtt
    45241 gttggagatc ggacggaaca cctgcaccgc gccctcttcg gaaagctgta ccagcccttt
    45301 gagcagctgt ttttgcttca gcggatcttt caggcggata cgacggaaca gttctggtgc
    45361 gaagttcgga ataccggtga acttcatcat ctcaccctgg gtaaaggtgt cgccgatctg
    45421 aatagtgccg tggttgtgca ggccgaggat atcgcccgga tacgcttctt caacgtgcga
    45481 acggtcgccc gccataaagg tcagcgcgtc ggagataacc acatctttcg cagtacgcac
    45541 ctggcgcagc ttcatgcctt tttcatattt accggatact acgcgcataa acgccacgcg
    45601 gtcgcggtgt ttcgggtcca tgttggcctg aattttaaat acgaagccgg taaatttgtc
    45661 ttcgctcgcc tctacggtac gggtatcagt ctgacgcggc atcggcgcag gggcccactc
    45721 caccaggcca tccagcatat gatcaacgcc gaagttaccc agcgcagtac cgaagaatac
    45781 cggagtgatt tcgcccgcaa ggaacagttc tttgtcgaac tcgttagacg cgcctttcac
    45841 cagttccagt tcgtcacgta gctgctgtgc cagatcttca ccaaccgcag cgtcgagatc
    45901 cgggttattc agccctttaa caatgcggac ttcctgaatg gtgtggcctt taccgctctg
    45961 ataaagatag gtttcgtctt tataaaggtg gtaaacgcct ttaaacagct tgccgcagcc
    46021 aatcggccag gtgatcggtg cgcagccgat tttcagctcg ttctcaactt catcgagcag
    46081 ttccatcggg tcgcggatat cacggtcaag tttgttcata aaggtgagga tcggcgtgtc
    46141 gcgcagacgg gtaacttcca tcagcttgcg ggtacgatct tcaacacctt ttgcggcgtc
    46201 gataaccatc aggcagcagt ccaccgccgt cagggtacga taggtatctt ccgagaagtc
    46261 ttcgtgcccc ggggtgtcga gcaggttaac caggcaatcg tgatacggaa actgcatcac
    46321 agacgtagta atggagatcc cacgctgctt ttccatttcc atccagtccg acttagcgtg
    46381 ctggttggaa ccacggcctt ttactgtacc agcggtctga atggcctgtc cgaacagcag
    46441 caccttctcg gtgatggtag ttttaccggc gtccgggtga gaaataattg caaaggttct
    46501 tcttttcgct acctctgcgg ccaaaaggct tgtcataatt gttgtctctt tgattatgta
    46561 caatcatatg tacaaagaaa aattcatgcc gctgattgta cattttggag gtatcagagc
    46621 atagcagaac atgatcagta ggtgtatttg tagccaaaga acatttgtac atgcagcaat
    46681 ctttgttcta ttgacactgt ttattcatac agttaaaaat aacactgtat acaaacacag
    46741 tgtagaggga tttttatgcg tattgagatc tgcatagcca aagagaaaat gactaaaatg
    46801 ccaaacggtg ctgtggatgc gttaaaggaa gaattaactc gacgcatcag taagcgttat
    46861 gacgatgtag aggtgatcgt aaaagccacc agcaacgatg gcctttctgt tacgcgcacc
    46921 gccgataaag attcagctaa aacttttgtt caggaaactc tgaaagatac ctgggagtct
    46981 gctgacgagt ggtttgttca ctaattaaca cgtaaaatcg gtaacggccg gaaatcattc
    47041 aatactcgca ctatcggaag ttcaccagcc aaccgcagca cgttcttgca tacgacgtgt
    47101 ctgcggtttt tcaaccattc agatacagca ctctcatcac attcattctt ccgcattaaa
    47161 gtatatatga ccatatcacc ttaatatttt tcttcttcag ataaaaattg ttatctatgt
    47221 atacttttaa accgaatccg tgtagagtct ctacctaagt tagttcgcag ctaccccttc
    47281 agtttgcgcc attaacctcc tgacttttgc cgctatctgt aagcattcct gtatacctga
    47341 aactacaggg ttcatctacg aactttaaga acacccagga taaaaattgt caactatatt
    47401 atatataaca ccatattaat tcgaggctat atgaacagca tactgataat cacttcacta
    47461 cttatcatat tcagcatttt tggtcatgct ctaataaaat tagggattgg catatccaat
    47521 aacccggaca aaacagatgt ataagtcaac atatcctgaa tcagacatac aatattgcaa
    47581 tgaaaaccaa taatatttta aggaatatct tcatgaaatc aaaagacacc ctaaagtggt
    47641 tccctgcgca gcttcctgaa gtcagaatta tcctgggaga tgctgtcgtg gaagtagcaa
    47701 aacagggaag acctatcaat accagaacat tgcttgatta cattgaagga aacataaaga
    47761 aaaagtcatg gctggataac aaagaattat tacaaacagc gatatcagtt cttaaaga
//
LOCUS       DAZKHC020000026        47726 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZKHC020000026 DAZKHC020000000
VERSION     DAZKHC020000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 47726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.026"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            102..734
                     /gene="comR"
                     /locus_tag="ACHOSM_RS08840"
                     /old_locus_tag="pgaptmp_001768"
     CDS             102..734
                     /gene="comR"
                     /locus_tag="ACHOSM_RS08840"
                     /old_locus_tag="pgaptmp_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HGU4558313.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     ISTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(796..1335)
                     /locus_tag="ACHOSM_RS08845"
                     /old_locus_tag="pgaptmp_001769"
     CDS             complement(796..1335)
                     /locus_tag="ACHOSM_RS08845"
                     /old_locus_tag="pgaptmp_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU4558314.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1562..2866)
                     /gene="ndh"
                     /locus_tag="ACHOSM_RS08850"
                     /old_locus_tag="pgaptmp_001770"
     CDS             complement(1562..2866)
                     /gene="ndh"
                     /locus_tag="ACHOSM_RS08850"
                     /old_locus_tag="pgaptmp_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HGU4558315.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3274..3816)
                     /gene="ycfP"
                     /locus_tag="ACHOSM_RS08855"
                     /old_locus_tag="pgaptmp_001771"
     CDS             complement(3274..3816)
                     /gene="ycfP"
                     /locus_tag="ACHOSM_RS08855"
                     /old_locus_tag="pgaptmp_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HGU4558316.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3839..4864)
                     /gene="nagZ"
                     /locus_tag="ACHOSM_RS08860"
                     /old_locus_tag="pgaptmp_001772"
     CDS             complement(3839..4864)
                     /gene="nagZ"
                     /locus_tag="ACHOSM_RS08860"
                     /old_locus_tag="pgaptmp_001772"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGU4558317.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4875..5699)
                     /gene="thiK"
                     /locus_tag="ACHOSM_RS08865"
                     /old_locus_tag="pgaptmp_001773"
     CDS             complement(4875..5699)
                     /gene="thiK"
                     /locus_tag="ACHOSM_RS08865"
                     /old_locus_tag="pgaptmp_001773"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HGU4558318.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5680..6321)
                     /gene="lpoB"
                     /locus_tag="ACHOSM_RS08870"
                     /old_locus_tag="pgaptmp_001774"
     CDS             complement(5680..6321)
                     /gene="lpoB"
                     /locus_tag="ACHOSM_RS08870"
                     /old_locus_tag="pgaptmp_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HGU4558319.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6335..6712)
                     /locus_tag="ACHOSM_RS08875"
                     /old_locus_tag="pgaptmp_001775"
     CDS             complement(6335..6712)
                     /locus_tag="ACHOSM_RS08875"
                     /old_locus_tag="pgaptmp_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HGU4558320.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLVAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPGSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6715..7074)
                     /gene="hinT"
                     /locus_tag="ACHOSM_RS08880"
                     /old_locus_tag="pgaptmp_001776"
     CDS             complement(6715..7074)
                     /gene="hinT"
                     /locus_tag="ACHOSM_RS08880"
                     /old_locus_tag="pgaptmp_001776"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HGU4558321.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7408..9597
                     /gene="fhuE"
                     /locus_tag="ACHOSM_RS08885"
                     /old_locus_tag="pgaptmp_001777"
     CDS             7408..9597
                     /gene="fhuE"
                     /locus_tag="ACHOSM_RS08885"
                     /old_locus_tag="pgaptmp_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HGU4558322.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQHIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGTPRNFSITSTYQF"
     gene            complement(9657..11090)
                     /gene="ptsG"
                     /locus_tag="ACHOSM_RS08890"
                     /old_locus_tag="pgaptmp_001778"
     CDS             complement(9657..11090)
                     /gene="ptsG"
                     /locus_tag="ACHOSM_RS08890"
                     /old_locus_tag="pgaptmp_001778"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HGU4558323.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SYVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11385..12182)
                     /locus_tag="ACHOSM_RS08895"
                     /old_locus_tag="pgaptmp_001779"
     CDS             complement(11385..12182)
                     /locus_tag="ACHOSM_RS08895"
                     /old_locus_tag="pgaptmp_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4558324.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12193..13197)
                     /gene="holB"
                     /locus_tag="ACHOSM_RS08900"
                     /old_locus_tag="pgaptmp_001780"
     CDS             complement(12193..13197)
                     /gene="holB"
                     /locus_tag="ACHOSM_RS08900"
                     /old_locus_tag="pgaptmp_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGU4558325.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13194..13835)
                     /gene="tmk"
                     /locus_tag="ACHOSM_RS08905"
                     /old_locus_tag="pgaptmp_001781"
     CDS             complement(13194..13835)
                     /gene="tmk"
                     /locus_tag="ACHOSM_RS08905"
                     /old_locus_tag="pgaptmp_001781"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4558326.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13825..14847)
                     /gene="yceG"
                     /locus_tag="ACHOSM_RS08910"
                     /old_locus_tag="pgaptmp_001782"
     CDS             complement(13825..14847)
                     /gene="yceG"
                     /locus_tag="ACHOSM_RS08910"
                     /old_locus_tag="pgaptmp_001782"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HGU4558327.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14850..15659)
                     /gene="pabC"
                     /locus_tag="ACHOSM_RS08915"
                     /old_locus_tag="pgaptmp_001783"
     CDS             complement(14850..15659)
                     /gene="pabC"
                     /locus_tag="ACHOSM_RS08915"
                     /old_locus_tag="pgaptmp_001783"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HGU4558328.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15779..17020)
                     /gene="fabF"
                     /locus_tag="ACHOSM_RS08920"
                     /old_locus_tag="pgaptmp_001784"
     CDS             complement(15779..17020)
                     /gene="fabF"
                     /locus_tag="ACHOSM_RS08920"
                     /old_locus_tag="pgaptmp_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGU4558329.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17108..17344)
                     /gene="acpP"
                     /locus_tag="ACHOSM_RS08925"
                     /old_locus_tag="pgaptmp_001785"
     CDS             complement(17108..17344)
                     /gene="acpP"
                     /locus_tag="ACHOSM_RS08925"
                     /old_locus_tag="pgaptmp_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU4558330.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17555..18289)
                     /gene="fabG"
                     /locus_tag="ACHOSM_RS08930"
                     /old_locus_tag="pgaptmp_001786"
     CDS             complement(17555..18289)
                     /gene="fabG"
                     /locus_tag="ACHOSM_RS08930"
                     /old_locus_tag="pgaptmp_001786"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HGU4558331.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISYYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18302..19231)
                     /gene="fabD"
                     /locus_tag="ACHOSM_RS08935"
                     /old_locus_tag="pgaptmp_001787"
     CDS             complement(18302..19231)
                     /gene="fabD"
                     /locus_tag="ACHOSM_RS08935"
                     /old_locus_tag="pgaptmp_001787"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGU4558332.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19247..20200)
                     /gene="fabH"
                     /locus_tag="ACHOSM_RS08940"
                     /old_locus_tag="pgaptmp_001788"
     CDS             complement(19247..20200)
                     /gene="fabH"
                     /locus_tag="ACHOSM_RS08940"
                     /old_locus_tag="pgaptmp_001788"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGU4558333.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20268..21338)
                     /gene="plsX"
                     /locus_tag="ACHOSM_RS08945"
                     /old_locus_tag="pgaptmp_001789"
     CDS             complement(20268..21338)
                     /gene="plsX"
                     /locus_tag="ACHOSM_RS08945"
                     /old_locus_tag="pgaptmp_001789"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGU4558334.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21419..21592)
                     /gene="rpmF"
                     /locus_tag="ACHOSM_RS08950"
                     /old_locus_tag="pgaptmp_001790"
     CDS             complement(21419..21592)
                     /gene="rpmF"
                     /locus_tag="ACHOSM_RS08950"
                     /old_locus_tag="pgaptmp_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGU4558335.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21644..22165)
                     /gene="yceD"
                     /locus_tag="ACHOSM_RS08955"
                     /old_locus_tag="pgaptmp_001791"
     CDS             complement(21644..22165)
                     /gene="yceD"
                     /locus_tag="ACHOSM_RS08955"
                     /old_locus_tag="pgaptmp_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HGU4558336.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22364..22948
                     /gene="yceF"
                     /locus_tag="ACHOSM_RS08960"
                     /old_locus_tag="pgaptmp_001792"
     CDS             22364..22948
                     /gene="yceF"
                     /locus_tag="ACHOSM_RS08960"
                     /old_locus_tag="pgaptmp_001792"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HGU4558337.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23060..24019)
                     /gene="rluC"
                     /locus_tag="ACHOSM_RS08965"
                     /old_locus_tag="pgaptmp_001793"
     CDS             complement(23060..24019)
                     /gene="rluC"
                     /locus_tag="ACHOSM_RS08965"
                     /old_locus_tag="pgaptmp_001793"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HGU4558338.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24592..27777
                     /gene="rne"
                     /locus_tag="ACHOSM_RS08970"
                     /old_locus_tag="pgaptmp_001794"
     CDS             24592..27777
                     /gene="rne"
                     /locus_tag="ACHOSM_RS08970"
                     /old_locus_tag="pgaptmp_001794"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HGU4558339.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27974..28927)
                     /gene="flgL"
                     /locus_tag="ACHOSM_RS08975"
                     /old_locus_tag="pgaptmp_001795"
     CDS             complement(27974..28927)
                     /gene="flgL"
                     /locus_tag="ACHOSM_RS08975"
                     /old_locus_tag="pgaptmp_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HGU4558340.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYALARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQINK"
     gene            complement(28939..30582)
                     /gene="flgK"
                     /locus_tag="ACHOSM_RS08980"
                     /old_locus_tag="pgaptmp_001796"
     CDS             complement(28939..30582)
                     /gene="flgK"
                     /locus_tag="ACHOSM_RS08980"
                     /old_locus_tag="pgaptmp_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HGU4558341.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDVYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30648..31589)
                     /gene="flgJ"
                     /locus_tag="ACHOSM_RS08985"
                     /old_locus_tag="pgaptmp_001797"
     CDS             complement(30648..31589)
                     /gene="flgJ"
                     /locus_tag="ACHOSM_RS08985"
                     /old_locus_tag="pgaptmp_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HGU4558342.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTATSAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31589..32686)
                     /gene="flgI"
                     /locus_tag="ACHOSM_RS08990"
                     /old_locus_tag="pgaptmp_001798"
     CDS             complement(31589..32686)
                     /gene="flgI"
                     /locus_tag="ACHOSM_RS08990"
                     /old_locus_tag="pgaptmp_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HGU4558343.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSLVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQINLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32698..33396)
                     /gene="flgH"
                     /locus_tag="ACHOSM_RS08995"
                     /old_locus_tag="pgaptmp_001799"
     CDS             complement(32698..33396)
                     /gene="flgH"
                     /locus_tag="ACHOSM_RS08995"
                     /old_locus_tag="pgaptmp_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HGU4558344.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33449..34231)
                     /gene="flgG"
                     /locus_tag="ACHOSM_RS09000"
                     /old_locus_tag="pgaptmp_001800"
     CDS             complement(33449..34231)
                     /gene="flgG"
                     /locus_tag="ACHOSM_RS09000"
                     /old_locus_tag="pgaptmp_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HGU4558345.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMFPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34369..35124)
                     /gene="flgF"
                     /locus_tag="ACHOSM_RS09005"
                     /old_locus_tag="pgaptmp_001801"
     CDS             complement(34369..35124)
                     /gene="flgF"
                     /locus_tag="ACHOSM_RS09005"
                     /old_locus_tag="pgaptmp_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HGU4558346.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNVGRANQLLSMS"
     gene            complement(35144..36352)
                     /gene="flgE"
                     /locus_tag="ACHOSM_RS09010"
                     /old_locus_tag="pgaptmp_001802"
     CDS             complement(35144..36352)
                     /gene="flgE"
                     /locus_tag="ACHOSM_RS09010"
                     /old_locus_tag="pgaptmp_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HGU4558347.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(36377..37072)
                     /gene="flgD"
                     /locus_tag="ACHOSM_RS09015"
                     /old_locus_tag="pgaptmp_001803"
     CDS             complement(36377..37072)
                     /gene="flgD"
                     /locus_tag="ACHOSM_RS09015"
                     /old_locus_tag="pgaptmp_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HGU4558348.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37084..37488)
                     /gene="flgC"
                     /locus_tag="ACHOSM_RS09020"
                     /old_locus_tag="pgaptmp_001804"
     CDS             complement(37084..37488)
                     /gene="flgC"
                     /locus_tag="ACHOSM_RS09020"
                     /old_locus_tag="pgaptmp_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HGU4558349.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37492..37908)
                     /gene="flgB"
                     /locus_tag="ACHOSM_RS09025"
                     /old_locus_tag="pgaptmp_001805"
     CDS             complement(37492..37908)
                     /gene="flgB"
                     /locus_tag="ACHOSM_RS09025"
                     /old_locus_tag="pgaptmp_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HGU4558350.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38063..38722
                     /gene="flgA"
                     /locus_tag="ACHOSM_RS09030"
                     /old_locus_tag="pgaptmp_001806"
     CDS             38063..38722
                     /gene="flgA"
                     /locus_tag="ACHOSM_RS09030"
                     /old_locus_tag="pgaptmp_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HGU4558351.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTSPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQIVSGVVDADGNILIN
                     L"
     gene            38798..39091
                     /gene="flgM"
                     /locus_tag="ACHOSM_RS09035"
                     /old_locus_tag="pgaptmp_001807"
     CDS             38798..39091
                     /gene="flgM"
                     /locus_tag="ACHOSM_RS09035"
                     /old_locus_tag="pgaptmp_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HGU4558352.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39096..39512
                     /gene="flgN"
                     /locus_tag="ACHOSM_RS09040"
                     /old_locus_tag="pgaptmp_001808"
     CDS             39096..39512
                     /gene="flgN"
                     /locus_tag="ACHOSM_RS09040"
                     /old_locus_tag="pgaptmp_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HGU4558353.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39552..41087)
                     /gene="murJ"
                     /locus_tag="ACHOSM_RS09045"
                     /old_locus_tag="pgaptmp_001809"
     CDS             complement(39552..41087)
                     /gene="murJ"
                     /locus_tag="ACHOSM_RS09045"
                     /old_locus_tag="pgaptmp_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HGU4558354.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41197..42120)
                     /locus_tag="ACHOSM_RS09050"
                     /old_locus_tag="pgaptmp_001810"
     CDS             complement(41197..42120)
                     /locus_tag="ACHOSM_RS09050"
                     /old_locus_tag="pgaptmp_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4558355.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDGTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42122..42769)
                     /locus_tag="ACHOSM_RS09055"
                     /old_locus_tag="pgaptmp_001811"
     CDS             complement(42122..42769)
                     /locus_tag="ACHOSM_RS09055"
                     /old_locus_tag="pgaptmp_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HGU4558356.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42780..43364)
                     /gene="rimJ"
                     /locus_tag="ACHOSM_RS09060"
                     /old_locus_tag="pgaptmp_001812"
     CDS             complement(42780..43364)
                     /gene="rimJ"
                     /locus_tag="ACHOSM_RS09060"
                     /old_locus_tag="pgaptmp_001812"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HGU4558357.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43600..44807
                     /gene="mdtH"
                     /locus_tag="ACHOSM_RS09065"
                     /old_locus_tag="pgaptmp_001813"
                     /pseudo
     CDS             43600..44807
                     /gene="mdtH"
                     /locus_tag="ACHOSM_RS09065"
                     /old_locus_tag="pgaptmp_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
     gene            44871..45518
                     /gene="grxB"
                     /locus_tag="ACHOSM_RS09070"
                     /old_locus_tag="pgaptmp_001814"
     CDS             44871..45518
                     /gene="grxB"
                     /locus_tag="ACHOSM_RS09070"
                     /old_locus_tag="pgaptmp_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HGU4558358.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45652..46215
                     /locus_tag="ACHOSM_RS09075"
                     /old_locus_tag="pgaptmp_001815"
     CDS             45652..46215
                     /locus_tag="ACHOSM_RS09075"
                     /old_locus_tag="pgaptmp_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4558359.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTDL"
     gene            46318..47364
                     /gene="pyrC"
                     /locus_tag="ACHOSM_RS09080"
                     /old_locus_tag="pgaptmp_001816"
     CDS             46318..47364
                     /gene="pyrC"
                     /locus_tag="ACHOSM_RS09080"
                     /old_locus_tag="pgaptmp_001816"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGU4558360.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEATVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREDQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47438..47683
                     /gene="dinI"
                     /locus_tag="ACHOSM_RS09085"
                     /old_locus_tag="pgaptmp_001817"
     CDS             47438..47683
                     /gene="dinI"
                     /locus_tag="ACHOSM_RS09085"
                     /old_locus_tag="pgaptmp_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HGU4558361.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11524 a        12906 c        11873 g        11423 t
ORIGIN      
        1 ttaaatagaa agcaattatt tttatggggt ctggactggt gaacggaggt gattattttt
       61 ataataacct tacattcaca gtcaccaggt acaacggcat catggcaact gactcaacac
      121 aatgtgtaaa aaaaagccgt ggccgcccaa aagtgttcga cagggatgcc gcgcttgata
      181 aggccatgaa attgttctgg caacacggtt atgaagcgac ttctcttgcg gacctcgtcg
      241 aagcgaccgg agccaaagcg cccacgctat acgcggaatt taccaacaaa gaggggttat
      301 ttcgtgccgt tctcgaccgc tatatcgatc gttttgccgc taagcatgaa gcacagctgt
      361 tttgtgaaga gaaaagcgtg gagtctgcgc tggctgacta ttttgctgcc atcgccaact
      421 gctttaccag caaagacacc ccggcaggct gcttcatgat caacaactgc accaccctct
      481 ccccagattc aggagatatc gccaatacgt tgaaatcacg ccatgcgatg caagagcgca
      541 ctttgcagca gtttttatgt caacgacaag cgcgcgggga aatctcgacc cactgtgacg
      601 tgacacatct ggcagaattc cttaattgta ttattcaggg gatgtcgatc agcgcacgcg
      661 aaggtgcatc gctggaaaaa ctgatgcaga ttgccggaac gactttgcgt ttatggcccg
      721 aactggtgaa ataagtcgca aaaaaagccc ctcagcctga gcgaatgagg ggccaaattg
      781 cagagtacag ccttgttata ctttgttatt caaaatcagt tgcccgttac tatctagcgg
      841 gatctgcgta cccggatcgc ggtccatgcg gattttgccc tgctgatcgc caatcttata
      901 ggtcacatca taaccgagca ttttttctga cttgtcatac accgttttac aacgctgttg
      961 cgtagtcgtg taagtatcgc tttcctggag agagccctgg atctggttac cggcatatcc
     1021 accacccagc gcccccacaa cagtggcgac atctttacca cgaccaccac caaactgatg
     1081 ccctatcacg ccgccagcaa cagcgccgag caccgacccg gtaatgcgat tttcatcctg
     1141 caccggtcga cgatgggtca ctgtgacgtt gcgacactcc tgacgcggtg ttttaaccgt
     1201 ttccttgatt ggggttgcag aaacaacctg agcgtattgc gggccccgtt caaacacgtt
     1261 cagactggcc actgccgcta cgcccagcgc agctgcgaca ccaatcccga tacccgccaa
     1321 cattgattta ttcacgggaa cacctccttc tttgcagtta tgccaatcat gcaagcgaag
     1381 gagaaaagca gctatcagac agaagatgaa aagcgcggga atggctggaa atacttaagg
     1441 attcggagga gtcttaagga atgcctgatg cgacgcttaa cgcgtcttat caggcctacc
     1501 gctcgtacca tttgtccagg gcggatgagg cgtttatgcc acatccgcca gtgtacgtcg
     1561 attaatgcaa cttcaaacgc ggacggataa cgcggttaat actccccacc agcatcatta
     1621 atccggtttt aaagtaacca tgcagcgcaa tctgatgcat tcggtatagc gagatatata
     1681 caaagcgcgc aattcgtcct tcaatcatca ttgagccgcg cgtcaggtta cccatcaggc
     1741 taccaacggt ggagaagttc gacagcgata ccagcgaacc atgatcttta tactgataat
     1801 ttttcagcgg cttaccgttc atctgcgcca gaatgttgtt cattgcgcaa gtcgccatct
     1861 ggtgtgcagc ctgagcacgc ggcggaacaa agcccccttc cggacgcggg catgacgcgc
     1921 agtcgccaat agcgtaaatg tctggatcgc gagtggtttg cagcgtcggt tccaccacca
     1981 gctggttgat acggttagtt tcaagaccac cgatatcttt caggaagtct ggcgctttga
     2041 tcccggctgc ccataccatc agatcagcct caatatattc gccatcttta gtgtgcaggc
     2101 cgccttcatc agcactggtg accatggttt gcgtcagcac gcgaacgcca agtttcgtta
     2161 gctcgttgtg ggccgcagca gagatacgtg gcggtaatgc aggcaaaata cgttctcccg
     2221 cttctaccag cgttacgttc agggcttcgt tggtcaggcc tttgtaaccg tagctgtgca
     2281 gttgcttgac cgcgttgtgc aattcagcgg agagttctac acccgtcgcg ccgccgccga
     2341 caatcgcaat gttcactttg ccatttgcgc ccaggttggc agagtatttc aggaacagat
     2401 tcagcatctc ctggtggaag cgacgcgcct ggtgcgggtt atcgaggaaa atgcagttct
     2461 ctttgacgcc tggcgtattg aaatcgttag aggtgctacc cagcgccatg accagggtgt
     2521 cataggcgat tttacgttcc ggaaccagca gttcaccttt ctcgtcgcgt agttctgcaa
     2581 tagtgattgt tttcgcttca cgatcaatat caatgacgga acccaactgg aactggaaac
     2641 catgattgcg cgcatgggcc agatagctca acgcatcgac gccttcatca agcgagccag
     2701 tcgccacttc gtgcagcagc ggtttccaca ggtggctgtg gttacgatcg accagcgtaa
     2761 ttttggcttt tttcttacgt cccagcttat gccccagctg tgttgccatt tccagcccac
     2821 cagcaccgcc gccgacaatc acaatctttt tcaatggcgt agtcaacgtg acccccttaa
     2881 atttattaac caattgttaa ttaatggtta ttaacatagc tcttaattaa caacaggtta
     2941 cagcaatgaa aatgttcttc gctattgaga ataacatgaa tggtgcattg gtcataccaa
     3001 aattgatgtt aatcaagttt tgttgctgaa aagataggcg actttaccgg gtgagatcaa
     3061 aaaatagagt ttattgttta ttatttgagc aaattcggaa gagtaatata gggtgaagcg
     3121 tatatcgagg gcgtttggtc gtcctgataa gatgcgcagc atcgcatcag gcattgatgc
     3181 tcgactgtcg gatgcggcgt ggacgcctta tccgacttac agaattcgga gcgcatcagg
     3241 taccggcaag gttacctgat gcgacgaggg catttacccg agggttttga acgctttaat
     3301 gcgctgtaaa tgcggggaga tattcttgaa tttgtgcgtc tgctcttcgt cccagacaat
     3361 ctcgtaataa tgatgcaact cttcagatgt ccgctggctg ttaagtgctt catcattacg
     3421 cgacaaaata accagacaac gatcgcgatt cttctcgcgg aagttggtca cacacttagt
     3481 ggcaatatcg gcatactctt ccgggcgatc aatcttgcct tccatgttct cataagggaa
     3541 caaattaggg ttgaagatca cctggcggat gtcgcaaaga aaaccaatcc gttccgccca
     3601 gtatccgccc aggccaacgc cgcaaattag cggacgctcg tcaacgttca gttgcaacat
     3661 tttgtccact tctttaagca gatgctgcat atcatgtttc ggatgccgcg tactgtagct
     3721 tatcaagcgt acatccgggt caataaactg caattgtaag actttctcgt ggttacccgg
     3781 actgttagag tcaaaaccgt gtaaatagat aatcatcgct tcctcacata agccagggtt
     3841 agtgacctgc tttctcttcc tgccagcgtt cgtgtaactg attcagacgg gtgctgatcg
     3901 ctttccagcg agccgagtcc atcagttcct gtcgcgaaaa tgaaccttta tgatacaaac
     3961 gtgtaacacg ttctgccttg atcggtgaca gattatctaa cacgctgacg gcccctttac
     4021 gattattgca gaccaggatc atatcgcaac ccgcatccag tgatgcctgc ccgcgttcgg
     4081 cataactgcc cataatcgcg gcaccttcca tcgataaatc gtcagagaaa atcacgccgt
     4141 caaaacccag ttcctgacgc aaaacggttt tcagccagta gggagaaccg ctcgccggac
     4201 gcggatcaac atcactgtag atcacatgcg caggcataat ggcgtcgagt ttattttcgc
     4261 ggattaagga actgaagacc gacatatctt tagcgcgaat ctccgcttgt ggacgtggat
     4321 cgcacggtgt ttctttgtgt gagtctgccg ttactgcacc gtgtcctggg aagtgtttcc
     4381 cggtcgtttt cattccggct tcatgcatac catcaataaa ccggctggca attgccaggg
     4441 ctttttgtgg atcggcatga taagaacgct cgccaatcgc cgcgctgata tgcccgacat
     4501 ccagcactgg cgcaaagctg atatcaatat ccatagcgat catttcgctg gccatcagcc
     4561 aacccgcctc ttgcgccagt ttgccgccct cttccattcc tgacagcgca gcgaatgatt
     4621 gcgccgctgg caagcgggta aaaccttcac gaaaacgctg cacgcgtcca ccttcctgat
     4681 ctaccgccac caccagatga ttgcgcgaag ctgcgcggat ctggcgcacc agttcacgta
     4741 actgggcagg atcatgatag ttacgcgtaa agagaatcag ccctcccacc agcggatgcg
     4801 caagtatttc acgctcttcc gcgtccagtt cgtaaccttt gacatccaac attactggac
     4861 ccacactgct ctccttattg ttttattaat agctgccgcc aggtgtcatc ggccagcctg
     4921 ataaattgtt gatcgccggt ttgtcgccag cggtactcaa accaccctgc tttgagcatc
     4981 agcagccagg gaaaccatcg cctgacctga cgccataatt gcgccggata aatcttcgcg
     5041 cgagtggcat agtcattgac caattgccgg tgctgttcag tattttccac ccacaccgcc
     5101 gccagttcca gcgcgatatc accatctccg gcatactccc agtcgatgag ttttaaccct
     5161 gacgcgctat gcactaaatt tccggcgtgg acatccatat gcaatggact taagcgtaaa
     5221 ggccgtggtt cccgcgcttt gcgcagacgt tttaacattc gcagccaacc cactgtccgc
     5281 cgcgccggat cgctttgctg ccagtacagt tccagtaacg gcaacagcgt tattcgccag
     5341 ccaaaacgtg gttgttgatg tagataatac agcaagcctg ccagttcgtt ggtatccggc
     5401 aaatacgttt ttaccgcgcc gggcagatag tcgactacca tccagtcacg gagatataaa
     5461 tgcggcttcg gtgcaatgct tgcgggtagt tgtgataaag cccgatactg gcgcaagaac
     5521 gcggactgcg gcgcatcagg atcgtgcggc tgacgcacaa caaaacgctg gccctgatgt
     5581 tcaatgagaa aactcccgcc actaagccca ctattaaacg tcgtgacggg atgaaactgc
     5641 gggaaaaagc gcgacagcaa ttcgtcgcgc atgatgggat tattgctgcg aaacggcacc
     5701 tttacctgac cagataattt cgcccgtctg caccagcatc agctgcattt gtagggtcgg
     5761 agcgttaacg ttgccagagg cgctggagta cagcacgtaa tgagcgccga cattgcgggc
     5821 aatgcctatg gctttactac gggtacctaa actgtcctgc ggcgacaaac ctaactgttg
     5881 cttagccatc gacagctgct gggcggaaac cagggtaaat ttcccgttat tagccagcgc
     5941 atttcgcagc gtttcggtcg cttctgcggc attcagcgaa ccgttagtac ggttgttaac
     6001 gctatcaacc agcaggacgc tacccgcagt caccccgtca gccccaagca tcttactgac
     6061 catcggctgc attgcgccat tccagtcata atggcgaata tgcggcgcag gcggtgccgt
     6121 ttgatcttcg tgctcaattg ggcctggctg ctgcgggatc gtcggcaccg agggcactgt
     6181 ggggacaggc tgttgtggct cggctggttg ttccggcgct ggtttcactt cttctaccgg
     6241 tgcaggttca cgttgcccca cacacccggc gagaaacatc gccagcgcgg taatcaaggc
     6301 gtagcgactc atttttgtca tcaagattca ccccttacaa atatagataa agtctgactt
     6361 tgtgcgcccc cagaaaattg gcgctgccgt acagcgttac cgccgaatgt gcgggaatgc
     6421 taacgctgcc tggcctttcc agaggatgca tctccagccc tctggcgtca taccagtaaa
     6481 aacgataatg aacggtaacg ggttcttgcc tttcgttata aagcgttgag gatgctgaag
     6541 gttgaatatc agacgtcgaa aggacgggct tttctgcact gatgcccgca accaataaag
     6601 atgactccat caccagcgat tgctcatcat tcaccggaat ttccggatgt gaacgacagc
     6661 ccaccagcag taacaacgcc agagacacca gcccaaagca tccttttctc atcgttacag
     6721 acctttatgt gccagcattg gtcccagcgg acggccaccc aacaagtgca tatggatgtg
     6781 gtaaacctct tgtccgccat ggcggttggt attcatgatc agacgatagc catcttcggc
     6841 aataccttct tgctcagcaa tttttgccgc tacggtgatc atgcgtccca gcgcctgctc
     6901 atgctcagct gagacgtcgt tcacagtcgg aatgaggata ttcggaatga tcagaatatg
     6961 cgttggcgct tgcggcgaaa tatcgcgaaa cgccgttacc agatcatcct ggtagacgat
     7021 atcggagggg atctcacgac gaataatttt gctgaatata gtttcttctg ccacgacatt
     7081 ttcctttttc aaatagccat gcgttgcgcc atgctacgcc gcgccacact gcgagtatag
     7141 agtatggtcg agttaaacct gctctttcaa ccttaactca cgatttctta agcaaaaaat
     7201 cgttttgctg ccgtatatat cgccattatt cccatttctg cctgcgaatg cgctgaaaag
     7261 acattctctg tttggtttac acgacaaata tgaatgcgta tatttctcat ttgcatttac
     7321 aaacaaaatt attcgcacac taaatataac cgctcatcac cctcctccgg catgagcctg
     7381 acgacgacat aaaccaagag atttcaaatg ctttcaacac aatttaacag ggataatcaa
     7441 tatcaagcca gcaccaaacc atcactactt gccggttgca tagcattggc actattacct
     7501 tctgccgctt ttgccgctcc agccactgaa gaaacggtga ttgttgaggg ttcagccaca
     7561 gctccagatg atggcgaaaa tgattacagc gtaacgtcta cctctgcggg taccaaaatg
     7621 cagatgactc aacgtgatat tcctcagtcg gtcactattg ttagccagca gcggatggaa
     7681 gatcagcagt tacaaacgct gggcgaagtg atggaaaaca cgctggggat cagcaaaagt
     7741 caggcggatt ccgatcgtgc tctttattat tcccgcggat tccagatcga taactatatg
     7801 gttgatggta tccccaccta ttttgaatcg cgctggaatc tgggcgacgc actttctgat
     7861 atggcactct ttgaacgcgt agaagtagtg cgtggcgcga caggactcat gaccgggacg
     7921 ggtaatccat ctgcggcaat taatatggtt cgaaaacacg cgaccagtcg tgaatttaaa
     7981 ggcgatgtct cggcggaata cggtagctgg aacaaagaac ggtatgtggc ggatttacaa
     8041 agcccactca ccgaagacgg taaaatccgc gcgcgaattg tcggcggcta ccagaataac
     8101 gactcatggc tggaccgcta caacagtgaa aagaccttct tctccggcat tgtcgatgct
     8161 gatttaggcg atcttacgac gctttcagcc ggttacgaat atcagcacat tgatgttaat
     8221 agcccaacct ggggcggttt accgcgctgg aatactgatg gcagcagcaa cagttacgat
     8281 cgcgcacgca gtaccgcacc tgactgggcg tacaacgata aagagatcaa caaggtcttt
     8341 atgaccctga agcagcggtt tgctgatacc tggcaagcga cgctgaatgc cacccactct
     8401 gaagtcgaat ttgacagcaa aatgatgtat gtcgatgcct atgtaaacaa agcggatggt
     8461 atgctggttg ggccatacag taattatgga cctggctttg attatgtcgg cggcaccggt
     8521 tggaacagtg gcaaacgtaa agttgatgcg ctggatttgt tcgctgacgg tagttatgaa
     8581 ttgtttggtc gtcagcacaa tctaatgttt ggtggcagtt acagcaaaca aaacaatcgt
     8641 tacttcagtt catgggccaa catcttcccg gatgaaattg gcagtttcta caactttaat
     8701 ggcaatttcc cacaaaccga ctggtcacca cagagcctgg cgcaggacga taccacacat
     8761 atgaaatcgt tatatgctgc cactcgtgtc acccttgccg atccgctgca tctgatcctc
     8821 ggcgcacgtt ataccaactg gcgggttgat acgctgactt acagcatgga gaaaaaccac
     8881 accacgcctt acgctggtct ggtgtttgac atcaatgaca actggtcgac ctacgccagc
     8941 tatacctcta ttttccagcc gcaaaatgat cgtgacagtt caggcaaata tctggctcca
     9001 atcaccggta acaactacga gctgggtctg aaatcggact ggatgaatag ccgtctgacc
     9061 accacattag ccatcttccg tattgagcag gataatgtcg ctcagtccac cggtacacct
     9121 atccccggca gcaacggcga aaccgcctat aaagcggtgg atgggacagt cagtaaaggg
     9181 gtggaatttg aactcaacgg cgcaattacc gacaactggc agctgacatt tggcgcaacg
     9241 cgctatattg cagaggataa cgaaggaaac gccgttaatc ctaatctgcc acgcaccacg
     9301 gttaaaatgt tcaccagcta tcggttgcct gtcatgccag agttgacagt cggcggtggt
     9361 gttaactggc aaaatcgcgt gtataccgac accgtgacac cgtatggcac cttccgcgcc
     9421 gagcaaggta gctacgcgct ggtggatctc ttcacccgct accaggtgac gaaaaacttc
     9481 tcgttacagg ggaacgtcaa taacctgttc gacaaaacct acgataccaa cgtggaaggt
     9541 tctatcgtct acggcacacc gcgtaatttc agcattacca gcacgtatca attctgaata
     9601 acacctgtaa aaaaggcagc catctggctg ccttagtctc cccaacgtct tacggattag
     9661 tggttacgga tgtactcatc catctcggtt ttcaggttat cggatttagt accgaaaatc
     9721 gcctgaacac cagaaccagc aaccactacg cccgctgcgc ccagtttctt caggccggcc
     9781 tgatccactt tagacacatc agcaacgctg acgcgcagac gggtaataca tgcgtcgagg
     9841 ttagtaatgt tttctttacc accaaatgca gcaaccagag ccggtgccat ttcgctggta
     9901 cctgtcgctt ttgcatcttc agtcgcgtct tcacgacccg gcgttttcag atccagtgct
     9961 ttaatcagca cgcggaagat ggtgtagtaa acaatcgcat aaccgatacc gacgatcggg
    10021 aacagccaca gtttgctgct gttaccagac agaacgatga agtcgatcag accgtgcgag
    10081 aacgacgtac cgtcacgcat ccccagaaga atacagattg ggaatgccag gcctgccaga
    10141 atcgcgtgga tgatgtacag gatcggcgca acgaacatga aggagaactc gatcggctcg
    10201 gtgataccgg tcaggaacga ggtcagcgcc gcggaaatca taataccgcc cactttcgcg
    10261 cggttttctg gtttagcaga gtgccagata gcaattgcgg cagctggcag accgtacatt
    10321 ttgaacagga agccaccaga cagtttaccc gcagtcgggt cacccgccat ataacgcgga
    10381 atgtcgccgt ggaaaacctg acctgctgcg ttggtgtatt caccaatctg catctggaaa
    10441 ggtacgttcc agatgtggtg cagaccaaac ggtaccaggc aacgttcgat gaaaccgtaa
    10501 atgccaaacg caactaccgg gttctggtaa gcagcccact gagagaaggt ctggattgca
    10561 gaaccaatcg gcggccaaat gaaggacagc acaacgccag taaagatggc agccaggcca
    10621 gaaatgatcg gcacaaagcg tttaccggca aagaagccaa gatactcagg cagcttaata
    10681 cggtagaaac ggttaaacat gtacgctgcg atcgcaccgg agataatccc tccgagtacg
    10741 ccagtatccg ccaggtgttt agaggcgatt tcttcagcag gtaaatgcag taccagtggc
    10801 gcaaccacgg ccatggtttt aaccatgatg ccataggcaa caactgcggc cagcgcggat
    10861 acgccatcgt tattggtaaa gccgagggcg acaccgatcg caaaaatcag tggcatgttt
    10921 gcaaagacgg aaccgcctgc ttctgccata acatacgata caacggcggg cagccagctg
    10981 aaattcgcgg aaccgacgcc cagcagaata cctgcgatag gcagtacgga taccggcagc
    11041 atcagcgatt taccgacctt ttgcaggtta gcaaatgcat tcttaaacat aattgagagt
    11101 gctcctgagt atgggtgctt tttttacgtt ctcacgcgtg gcaagggggg agagcctcgc
    11161 cgtgtacagg gcatctaagc gccctttatt tattacacag agtaaaataa ttcagtgcca
    11221 atttgtttga cggctatcac gtttcaactt cagatcattg gagaagttgc acattttgac
    11281 aaaacatatg tcacaatatt tcaaacgcgg ttattcaccg cccaattttc ggcggtgaac
    11341 tttactcgtt ttagccatta attacgagct ttaaaaaaac tcattcaacg gatggattga
    11401 aggcgggaag cgtcgatgtg aaacagacgg gcgaagttat cggtggttac ctgcgccagt
    11461 tcttcaacgg caacaccttt caacacagcc atgtattctg caacgtcacg aaccatcgca
    11521 ggttgattct cttttcctcg atgcggtacc ggcgcaaggt aaggtgagtc agtttccacc
    11581 agtaatcgat ccagggggac ataacgcgca gcatcgcgca gttgctccgc attacggaag
    11641 gtcacaatgc cggaaaagga gatgtaaaat ccgagatcca gtaatttacc cgccgtttct
    11701 ctgtcctctg taaaacagtg tagtacgcca ccgcaatccg tcactttttc ttcgcgcaga
    11761 attgccagcg tatcggcacg ggcgtcacgg gtatggacga taaccggctt gttcagttca
    11821 cgaccaatct ggatatgatg gatgaaggac tcttgctgac gtactttagt ttccggtgtg
    11881 taataataat ccagcccggt ttcacccagc gctacaacac cctcttctgc cgccagacgg
    11941 cgtaaatctt ctacatcgta gggatcattc tggttgagtg gatgcacgcc acaagaaaat
    12001 acgacgttgt cacgttcgcc taccagatcc cgcatatgta aataacccgg taatgttgtg
    12061 gcgactgcca gacaaaattt cacatcgcgt gcggcggctt tcgccagaac gtcatccacg
    12121 tccttatgca aagattcata atccagacca tcgagatggc agtgtgagtc gactaaaaac
    12181 atgatgtctc tcttaaagat gaggaaccgg tagcacaacg cccggttgca ggtaatgctc
    12241 aatacgcagt aaaagatcgg tgatgagaag ctcgcggttg atgcctgtga cagacattaa
    12301 ctgttcacga atgtggcaaa catcccccag tatagcctgc aggcgcgagg gagaaagatg
    12361 gtttgccagt tcggcgacca ggcccggcac atcaacattg gtcacctgcg cagcgccatg
    12421 atggcgtttt agcgcatcca tcagcaacgt tgccagccag tgtaaacgcg ccggagcttg
    12481 ttcatgatta agggccgcca gcagcgaata ccagtcgccc gatggcacgc tatatgccaa
    12541 cgcctgacac aatgtttcac gagcctgcca gttatctccc tgaaacaacg ccagtgccgc
    12601 gccaggcgaa ccggcgctta agcgcaatgc ggcaagtaat gcatcctgtg acattgtcac
    12661 ttcgcgtgaa agccaggtca cggcgtactg ttctggaggc ggcgcaaggt aatgtaaccg
    12721 acaacgacta cgtaatgttg ccagtaaacg ttcaggctcg cgggtagcca ggaaaaacca
    12781 ggtttctgct ggtggctctt caagcgtttt cagcaatgcg ttagccgcgg cgtcggtcag
    12841 taaggcagca tcggttaccc agacaacttt cgcaccacct aggcgtgcgt gctcattcag
    12901 cttttcggtg acctcacgta ccgcatcaat gcccagcgta tttttccctt tttcgggagc
    12961 cagggtgtag taatcgggat gcgtgccagc ctgcatcaac tgacatccac gacagtgacc
    13021 gcaacttttg tggccctgcg gttgttggca gagtaaataa cggctcaggg cgtagattaa
    13081 agcatcatcg cccatgcccg gtaacgcctg aatgagtagc gcatggtgac ctcttccggc
    13141 ctgatagctg gctaccagtt tttcgaagtc aggtcgtaac catggatacc atctcatgcg
    13201 tccaactcct tcacccagtg ggtcacggta gtgcggattg catccatcac ggcctccagc
    13261 ggctgggtgg catcaatggt atgaatactt ttatcttgtg cagccagttc cagatagcgg
    13321 gcgcgggtgc gattaaagaa atcgaaagac tcttgctcaa tacgatccag ctcgccgcgc
    13381 gcacgcgcgc gttttaagcc aacttccggg gtaacatcga gatagagcgt taagtcgggg
    13441 cgaaaatccc cgagaacagc atcacgcagt gttgccagca tatgttggtc aataccacgt
    13501 cctccgccct gatacgcctg agtggagaga tcgtggcgat cgccaatcac ccaggtgccg
    13561 ttagccagcg caggtttgat gaccgtttct accagttgaa cgcgcgcggc ataaaacatc
    13621 agaacttcgg ctttatcggt aatgacttca tcgcctaccg atttgatatc cagcaccagg
    13681 cttcttaact tttcggcaag ttgcgtaccg ccaggttccc gagtgaaaac catgtcgcgg
    13741 atacccagtt gctcgagcgt ctcaaccacc acattacgcg cggtagtttt gcctgcgcct
    13801 tccagcccct caatgacgat atacttactg cgcatttttt tccttaagca ctttcagata
    13861 atcctgcaca gacttgttat gactggcaag attggtatta aacgtgtgac cacctttacc
    13921 atcggccaca aaatagagat acggcgtttt tgccggatgc gcagcagcct tcagcgaatc
    13981 cgcccccggc gtagctatcg cacccggcgg caaaccggta atggtatagg tgttatacgc
    14041 tgtcggcgtt tccaggtctg cacgagaaag tttgccatta taacgctctc ccatcccgta
    14101 aatcacggtt gggtcggtct gcaggcgcat accaatgcgt aaacggttga taaataccga
    14161 ggcaacctga tcgcgttcac tggcaacggc ggtttctttt tcgataattg atgccatcgt
    14221 caccagctga tttttatctt tataaggcag accgtccgca cgcccttccc aggcgctatc
    14281 gaccgctttc accattttct tgtgcgctcg cttgagtaac gcgacatcgg tggtattggc
    14341 ggtatacatc caggtgtctg gccagaacca accttcaatc cactccgggt tttccagttc
    14401 aagtgcctgc gctacggtgg cgtacttatc gtcactcagc gtgtgcttga tatacggagc
    14461 ctcacgcaat tgcttgaggt agtcgctcag acgcatccct tctaccagtc gcagcgggaa
    14521 ctgtgcttct ttaccgcttt ccagcaattt cagcatctcg cgcacggtca tctgcggtgt
    14581 aaagcggtaa gtcccggctt taaagtgaga aagatccggt tcgatacgca gcaaccattg
    14641 aaaaacccgt gggcgattga tgatcttatc ggcataaagc tgttcaccaa gcgccagacg
    14701 tccggtccct ggcttcaggg taaatatcgt ctcttctttg ataagcaatt tgctgtcggc
    14761 aagatggcga accttccaga cgcccacacc agcggcgata cccagtacca ccagcaataa
    14821 caagataatc aataacactt ttttcatgac taattcgggc gctcacaaag tggggctaaa
    14881 tattcatata acgttgctga cgaaaaggag acatcgccac aggcacgtac gggcatcact
    14941 ggcattaacg cattacaaat aaccatctca tctgcctgca acgcctcttc cagagaggct
    15001 tgcacttcga caagctgata agaggattgt gccagcaaac ggatacagaa ttgtcgcata
    15061 atgccgttaa cacctgcctg atccagtcgc ggcgtataaa ctacattgcc cttccgccag
    15121 aacaaattag ccgcacagca ttccgtaacc cacccttcgc tgtcaaggac cagcgcctca
    15181 tcagcgtttg tctgctcaag atgagagcgg atcaatactt gctcaagccg attaagatgt
    15241 ttaatacctg caagatgagg attgcgcccc agccgcaccg ggcttagcgc caacgtcatc
    15301 ccttcgttac gcaaacggtc gtaatgtgca ggataaaccg taacggagag aatccgcgtt
    15361 gccggtccac tgttcaatgt gctgtaccct cgcccgccac taccgcgact gatcacgact
    15421 ttcagcacac cattttgctg ttctgctgcc agcgttttca tctcttgttc aagctgaggc
    15481 cagaagtcac agaaaatcat caaccgctga caagcatcct gtagtcgctg gatatgcgcc
    15541 gataacaaac tgactttacc gtcgataact ctggcggtgg taaaacaacc atcaccaaac
    15601 tgcgttgccc gatcgcttac tgccagcgat tcctgcttat aaccgttaat taagaacata
    15661 ccggctcctt atcagagaat tgttagtgtg gcagcatgtt cactacggaa caagtcggaa
    15721 taaaagctaa gaaaaaaggc ccgcaagcgg accttttata tgggtggaaa atgacaactt
    15781 agatcttttt aaagatcaaa gaaccattag tgccaccgaa gccgaaggag ttacacagcg
    15841 tgtattccat tccgctgacc tgacgtgctt catgcggtac gaaatccaga tcgcaacctt
    15901 catccgggtt atccaggttg atggttggcg gaacagcctg atcgcgcagc gccaggatgg
    15961 agtagataga ttctactgca cccgccgcac ctaacaggtg accggtcata gatttcgtgg
    16021 agcttaccaa cacacggctt gcagcttcac cgaagatggt tttcaccgcc tgcgcttcag
    16081 ctttatcgcc agccggcgta gaagtaccgt gcgcgttaac gtagccaatc tgactcgctt
    16141 caatgcctgc atcacgcaga gcatttgcca ttgccagagc tgcgcctgcg ccattttctg
    16201 gcggtgacgt catatgataa gcatcgctgc tcataccaaa gccgacgagt tcagcgtaaa
    16261 ttttcgcacc gcgttttttc gcgtgttcgt actcttcaag taccagcata ccggcaccat
    16321 cgcccagtac gaagccatca cgctctttat cccacgggcg gctcgccgct tgcgggttat
    16381 cattgcgggt agataatgca cgtgccgcgc caaaaccacc aacacccagc ggcgtactgg
    16441 ctttctctgc gccacctgca accatcacgt cagcatcgcc atacgcgata atacgcgcag
    16501 catggccaat gttgtgcacg ccggaagtac aggcggtcgc gatagagatg cttgggccac
    16561 gcaggccata catgatagtc agatgacctg ccaccatgtt cacaatcgtt gacggaacga
    16621 agaatgggct gatcttacgt ggaccaccgt tcatcagaga tgtgtggttt tcttcgatca
    16681 gtccgaggcc gccaatcccg gagccaattg cggcaccaat gcgggttgcg ttctcttccg
    16741 ttatttcaag gccagaatcc tgcatggcct gaacgccagc gacaattcca tattgaatga
    16801 aggcatccat cttgcgctgt tctttgcgcg agataatgtc ctcacagtta aaatccttta
    16861 ctaagccagc aaatttcgtt gcataggcgc tagtatcgaa atggtcgatt aggctgatgc
    16921 cactctgacc ggcaagcaga gctttccagg tagactctac ggtattgccg acaggagaca
    16981 acatgcccag tccggtcaca actacacgac gcttagacac gtttgtcctc cagggaggga
    17041 aaaaatgatt ctagtgggac aaaaagataa aactcaggcg gtcgaacgac cgcctggaga
    17101 tgttcactta cgcctggtgg ccgttgatgt aatcaatggc agcctgaacg gtggtgattt
    17161 tctcagcttc ttcgtccgga atctcagtat caaactcttc ttccagagcc attaccagct
    17221 caacggtgtc aagagaatcc gcgcccaggt cttcaacgaa agaagcattg ttggtaactt
    17281 cttcctgctt aacgcccagc tgttcgccga taattttctt aacgcgttct tcgatagtgc
    17341 tcatactctt aaatttccta tcaaaactcg ctttcgcgat ggttttcgta gtgtataaaa
    17401 tgttgaaaaa tttgcaacta aatcccggca ggtcttacca cgattttacg ttattttgag
    17461 gccttacccc ccaataacgc aaatcatttt gcactaatta ttactcataa ccacgcagac
    17521 tgcgcgcaac atgagcattt tgtgcaaatc gcggtcagac catgtacatc ccgccgttca
    17581 catgcaaagt ttcacccgtg atgtaagctg cttcgtcgga tgccaggaat gcaaccgcgt
    17641 tggcgatttc ctgtgcgccg ccgaggcgac ccgcaggaac ctgcgccagg atacccgcac
    17701 gctggtcatc gctcagcgca cgtgtcatgt ccgtttcaat aaagcccgga gcaacaacgt
    17761 ttacagtaat accgcgtgac gcaacttcgc gcgccagtga tttactgaag ccgatcaagc
    17821 ccgctttcgc cgcagcgtag ttggcctgac cgccatttcc catggtacca accacagaac
    17881 cgatagtgat aatacgacca tgacgctttt tcatcatagc gcgcattacc gcttttgaca
    17941 gacggaaaac agatgaaagg ttggtttcga taatatcgtt ccactcttca tctttcattc
    18001 gcattaacag gttatcacga gtgataccgg cattattgac caggatatcc acttcaccaa
    18061 attctgcgcg aattttttcc agaacagatt cgatagatgc cgggtcggtc acattcaaca
    18121 tcagaccttt gccgttagca cctaaataat aactgatcgc ctgagcgcca ttttcactgg
    18181 tcgcagtgcc aataactttc gcgccacggg ctgcgagcgt ttcagcaatt gcgcggccaa
    18241 ttccgcggct tgcaccggtt accagtgcga tttttccttc aaaattcatg attttcctct
    18301 tttaaagctc gagcgccgct gccatcgctg aaggttcgtt cagcgccgag gcggtcaggg
    18361 tgtcgacaat gcgtttcgtc aggccagtta gcactttgcc cgggccgact tcatagagat
    18421 gttctacgcc ttgcgctgcc atgtactcaa cagacttcgt ccactgaacc gggttataca
    18481 actgacgtac cagtgcgtca cgaatggcat caccattggt ttcgcatttc acatcaacgt
    18541 tattcacaac aggaactgtt ggtgcgttaa aggtgatttt cgctaattct actgccagtt
    18601 tgtcggctgc tggtttcatc agcgcacagt gagacggtac gctcactggt aacggcagcg
    18661 cgcgttttgc gcccgcagct ttacaggcag cgccagcacg ctcaaccgcc tctttatgac
    18721 cggcaataac cacctgtccc ggagagttaa agtttaccgg agaaacgacc tgaccttctg
    18781 cagcttcttc acacgctttc gcaatagacg catcatccag accgatgatt gccgccatag
    18841 cgcccgtgcc ttctggtacg gcttcttgca tgaacttgcc gcgcatctca accagacgca
    18901 ccgcatcagc gaaatcaatc acgccagcgc aaaccagcgc ggagtattcc cccaggctgt
    18961 gaccggccat cattgccggt gctttaccgc cctgctgctg ccatacgcga tacagcgcaa
    19021 cagatgcagt caacagcgct ggctgggttt gccaggtttt attcagttct tcagctggcc
    19081 cctgctgggt cagcgcccac aggtcgtagc ccagcgccgc agaagcttca gcaaacgttt
    19141 cttcgacaat tggatagctc gccgccatat cagccagcat tccaacggtt tgagaaccct
    19201 gtccagggaa cacaaatgca aattgcgtca tgttttaatc cttatcctag aaacgaacca
    19261 gcgcggagcc ccaggtgaat ccaccgccaa aggcttcaag cagaaccaac tgccccggct
    19321 taatgcgccc gtcgcgtaca gcttcatcca gcgcgcacgg gacagaggcc gcagaggtat
    19381 taccgtggcg atccagcgtc accacgacat tgtccataga cataccgagt tttttcgccg
    19441 ttgcactgat aatacgcagg ttagcctgat gcggaaccag ccagtccagt tgagaacggt
    19501 caagattatt cgccgccagc gtctcatcaa cgatgtgcgc cagttccgtt accgcaacct
    19561 tgaagacttc gttgcccgcc atcgtcagat gaattgaatt ctctggattc acgcggtcgg
    19621 cgtttggcag cgtcagcaat tcaccataac taccgtcggc atgcagatgg gtggagatga
    19681 ttcccggctc ttcagaggca gccagcaccg cagcgcccgc gccatcgcca aaaataataa
    19741 tagtcccacg atctgttgga tcgcaggtgc gcgccagtac atcggaaccg acgaccagag
    19801 catacttcac cgccccagat ttcacgtatt gatcggctac gcttaatgca taggtgaaac
    19861 ctgcgcaggc tgctgcaacg tcaaatgccg ggcaaccttt aatgcccaac atgctttgaa
    19921 tctgacaagc tgcgctcggg aaagcgtgcg tagcagaagt cgttgccaca acgatcaggc
    19981 caatctggtc tttctcaatg cccgccatct caattgcgcg tgtcgccgct tcaaagccca
    20041 tggttgaaac ggtttcgttt ggcgcggcaa tgtggcgttc gcggataccg gtacgagtga
    20101 caatccactc gtcagaggtg tccaccattt tttccaaatc ggcgtttgtc cgcacttgtt
    20161 cgggcagata gctgccagta ccaataatct tcgtatacat gtacgctcag tcacttttcg
    20221 gttatatacc gtcacttgca aactgcgagt tcgccggcag cgtcctgcta ccgcagagtt
    20281 ccgcttttgc caccgtccag cagctcaaaa ccagctgggt atacagattc caggcgagcg
    20341 gcaattcgct gaggaacttg tcgctgcacc gcctgcactg cctgttcaat cgcgaccgca
    20401 aaagctcgct gattggctgc accatgactt tttatcaccg tgccgcgcaa tcctaacaga
    20461 caggcgccgt tatactggtc ggggttgagg tgactgaatc gcctcgtcag gctcttttgt
    20521 agccaacgct ttaataacag tagccaccac gaccgttttt tcccttcacc ctgagatttc
    20581 agcagagaaa ggaacatcct gacaacacct tccatcgtct ttaatgtgac atttcctgta
    20641 aagccgtcac aaaccagcac atctgtcttg ccagttaaca actcattggc ttcaagatag
    20701 ccgatataat tgatagaagg gattgtttta agcaccgctg aggcatcccg aatactgtcg
    20761 agacccttta cttcttcttc accaatattg agcaacgcca cgcgaggatt gggaatttcc
    20821 accacctctt cagccagaac tgagcccata atggcaaatt gcaccaacat tgtactgtca
    20881 caatcgacgt tggcccctaa gtcaaggacc accgttttgc ccttttgctg atgtggtaat
    20941 accgtcacca gcgccggacg ctcaatcccc tccaggggct tgagtaataa ttttgccagc
    21001 cccatcagtg ccccggtatt accggcactg acacaggctt gcgctcgacc ttctttcacc
    21061 agctccaggg ccacgcgcat tgaactccca cgactggcgc ggatagcttg cgaaggccgg
    21121 gcatcactgg cgataactga ctgcgcagga ataatctgca gacgcgaacg ttgttcaaag
    21181 tcagctttag caagtaatgg cgtgatggcg tcgggattgc cgactaaaag aagagtgagt
    21241 tgcgaattag aattcagtgc ctgcaatgct gcaggcactg tcacggaagg gccaaaatcc
    21301 cctcccatga catctaacgc cagggttaga cgtgtcaagg tatcgtcgcc gcccggtttg
    21361 gtctttcccc agttgcctgg ggaaaatcct cactaagctt catcacgcag acgcgtgatt
    21421 acttagcgat gaccttgcgg ccgcggtagt aaccgtcggc agtgatgtgg tgacgcaggt
    21481 gtttttcacc agaagttttg tctacagaca ggctggtgac tgcggtcagc gcgtcatggg
    21541 aacgacgcat gccacgtttg gaacgggttg gtttattctg ttgtacggcc atggacctta
    21601 ctcctcaatt acggtttatc cccgatccaa cggggagcac caattactta cgctttaagc
    21661 tggctaatac ggcaaatggg tttggctttt gcacttcttc aggcagttca ccaaagacca
    21721 tgtccgcttc ggacacttca cagtgttcag aatcatgcac cggaactacc ggcaaggcga
    21781 ggatgatttc atcttcaacc attgcaagca gatcgatttc accgaattcg ttaacctcaa
    21841 tcggttcata cgcttccggc agtgcttcag cctgttcgtc tgaacgcaca ggactaaaac
    21901 aatacgttgt gtagacctga tgagtaaacg gcttcccgca acgctgacac tcgagcgtta
    21961 ccgtcacctt cgcatcgccg tttaacactg cgagacgttg gttatcgata gcgaacgaca
    22021 tggagcattc cacatcactg tccacactga ctacggattc ggcgacgcgc tcaacctgat
    22081 caggggtata gataccctgg taatcaaggc gtttttgagc cgtacgaacc ggatcgagag
    22141 tcaggggtaa ttttaccttt tgcatagggc gcgcatatta actttgtaac gtcatagagt
    22201 caaagaaaaa ggcagcctgt ggttgccttt tgccaataat tcgcacacat tgcgggttac
    22261 tgctttattt tcgttcagtg ctgataacga acatcaacct gcaaaacgaa tcgcatagtt
    22321 taaaatggct atcatcaata cgctatatct gatggaaaaa aatatgccta aacttatttt
    22381 agcctccacc tcgccatggc gtcgcgctct tctggaaaaa ctgcaaatct cttttgaatg
    22441 tgcagcacca gaggtcgacg aaaccccacg cagcgacgaa tcaccgcgac agttggtgct
    22501 tcgactggca caagaaaaag cgcaatctct ggcgtcacgt tatccggatc atttaattat
    22561 tggttcagac caggtatgtg ttcttgatgg cgaaatcacc ggtaaaccgt taacggaaga
    22621 aaatgcccgt ctgcaattac gcaaagccag cggcaatatc gtcaccttct atactggact
    22681 ggcgttgttt aattcggcga atgggcatct acaaacagaa gtggagcctt ttgacgtcca
    22741 tttccgtcac ctgagcgagg cggagattga taattacgtg cgtaaggagc atcccctgca
    22801 ctgcgcgggt agctttaaga gtgaaggatt tggcattacg ctgtttgagc gtttagaggg
    22861 acgtgaccct aacacgctgg ttggtttacc gctaatcgcg ctgtgtcaga tgttacgtcg
    22921 ggaagggaaa aacccgctga tgggatagtt gtttgcgccg gatgcggtta cgtcgcatcc
    22981 ggcaatcggt gcaggcggcc tgatgcggcg taaacgcctt atcaggccaa ccgcccatta
    23041 tcaggcttat atgcttgtat tagcgcgcgt tacgcagctt ttgcaaacaa cgcttcaaac
    23101 cttcatccat cggcgcttcg atacgcatca cttcaccggt ccccggatgg gtaaacttca
    23161 acgctgcggc gtgcaggaac agacgattta atcccgtgcc tgcttcagtg agctgtctgt
    23221 caaattcacg gtcaccgtag cgatcgtcaa aggcaatcgg atgacccgca tactgtgtat
    23281 gcacacggat ctgatgagta cgtccggtta ccgggctaca acgcaccagg gtggcaaatg
    23341 catagcgttc ttccacttta aagcgtgttt ccgacggttt gccttcctga ctgacacgca
    23401 cgatacgttc gccgctttgc agaatatttt tcaataacgg cgcttgaacg ctcttcacat
    23461 gcgactgcca ctgaccgcgc accagcgcca ggtaatcttt ttgcattcct ttttcacgta
    23521 attgctcatg cagagaacgc aacgccgagc gttttttcgc taccagcaac acacctgaag
    23581 tgtcccggtc gagacgatga accagttcaa ggaaccgcgc ttccgggcgc aacgcccgca
    23641 aaccttcgat aacgccgaag cttaaaccac tgccgccatg taccgccgta ccggaaggtt
    23701 tattcagcac caggatatga tcatcttcat ataagatgac gtccgccagc gccgccacct
    23761 tttgcagatg tggcgaaacc gcctcttctt cccgctcagc aacgcgaacc ggtgggatac
    23821 gcacctcatc acccgcttcg agtttatatt caggcttaat acgttttttg ttcacccgca
    23881 cttcaccttt acgcaaaata cggtaaatca tactttttgg tacgcctttc aattgggtac
    23941 gcaaaaagtt atcgatacgt tgccccgctt cgtcagcggt gatagcaaca atttttacgg
    24001 atggagtctc tgttttcatg gtgggcgatt ctaattagcc aacaggattc gcgccactca
    24061 tttttctatg cttatattta gttttgcacc ttattacttc actacgtgat cactttattg
    24121 atggttatta aaccaatcac cagcaagaag tgaaaaaact gtgagtaagc gggtgataaa
    24181 tggtaaaagt catcttgcta taacaaggct tgcagtggaa taatgaggcc gtttccgtgt
    24241 ccatccttgt taaaacaaga aattttacgg aataacccat tttgcccgac cgatcatcca
    24301 cgcagcaatg gcgtaagacg tattgatctt tcaggcagtt agcgggctgc gggttgcagt
    24361 ccttaccggt agatggaaat atttctggag agtaataccc agtctgtttc tttgataatt
    24421 gcgctgtttt tccgcatgaa aaacgggcaa ccgacactct gcgcctcttt gagctgacga
    24481 taaccgtgag gttggcgacg cgactagaca cgaggccatc ggttcacacc cggaaaggcg
    24541 ttactttgcc cgcagcttag tcgtcaatgt aagaataatg agtaagttac gatgaaaaga
    24601 atgttaatca acgcaactca gcaggaagag ttgcgcgttg cccttgtaga tgggcagcgt
    24661 ctgtatgacc tggatatcga aagtccaggg cacgagcaga aaaaggcaaa catctacaaa
    24721 ggtaaaatca cccgcattga accgagtctg gaagctgctt ttgttgatta cggagctgaa
    24781 cgtcacggtt tcctcccact aaaagaaatt gcccgcgaat atttccctgc taactacagt
    24841 gctcatggtc gtcccaacat taaagatgtg ttgcgtgaag gtcaggaagt cattgttcag
    24901 atcgataaag aagagcgcgg caacaaaggc gcggcattaa ccacctttat cagtctggcg
    24961 ggtagctatc tggttctgat gccgaacaac ccgcgcgcgg gtggcatttc tcgccgtatc
    25021 gaaggcgacg accgtaccga attaaaagaa gcactggcaa gccttgaact gcctgaaggc
    25081 atggggctta tcgtgcgcac cgctggcgtc ggcaaatctg ctgaggctct gcaatgggat
    25141 ttaagcttcc gtctgaaaca ctgggaagcc atcaaaaaag ccgctgaaag ccgcccggcc
    25201 ccgttcctga ttcatcagga gagcaacgta atcgttcgcg ctttccgcga ttacttacgc
    25261 caggacatcg gcgaaatcct tatcgataac ccgaaagtgc tcgaactggc acgtcagcat
    25321 atcgctgcat taggtcgccc ggatttcagc agcaaaatca aactgtacac cggcgagatc
    25381 ccgctgttca gccactacca gatcgagtcg cagatcgagt ccgccttcca gcgtgaagtc
    25441 cgtctgccat ccggtggttc gattgttatc gacagtaccg aagcgttaac tgccatcgac
    25501 atcaactccg cacgcgcgac ccgcggcggc gatatcgaag aaaccgcgtt taacaccaac
    25561 ctcgaagctg ccgatgagat tgctcgtcag ctgcgcctgc gtgacctcgg cggcctgatt
    25621 gttatcgact tcatcgacat gacgcctgta cgccaccagc gtgcggtaga aaaccgtctg
    25681 cgtgaagcgg tgcgtcagga ccgtgcgcgt attcaaatca gccatatttc tcgctttggc
    25741 ctgctggaaa tgtcccgtca gcgcctgagc ccatcactgg gtgaatccag tcatcacgtt
    25801 tgtccgcgtt gttctggtac tggcaccgtg cgtgacaacg aatcgctgtc gctctctatt
    25861 ctgcgtctga tcgaagaaga agcgctgaaa gagaacaccc aggaagttca cgccattgtt
    25921 cctgtgccaa tcgcttctta cctgctgaat gaaaaacgtt ctgcggtgaa tgcgattgaa
    25981 acacgtcagg acggtgttcg ctgcgtgatt gtgccaaacg atcagatgga aaccccgcac
    26041 taccacgtgt tgcgcgtgcg caaaggggaa gaaacgccga ccttaagcta catgctgccg
    26101 aagctgcatg aagaagcgat ggcgctgccg tctgaagaag agttcgctga acgtaagcgt
    26161 cctgaacaac ctgcactggc aacctttgcc atgccggatg tgccgccagc gccaacccca
    26221 gctgaacctg ccgcgactgt cgtagcccca gcaccgaaag ctgcaacggc aacaccagca
    26281 gctcctgcac aacctgggct gttaagccgc ttcttcggcg cactgaaagc gctgttcagc
    26341 ggtggtgaag aagccaaacc gaccgagcaa ccaacaccga aagcagaagc aaaaccggaa
    26401 cgtcaacagg atcgtcgcaa gcctcgtcag aacaaccgcc gtgaccgtaa tgagcgccgc
    26461 gacacccgta gcgaacgtac tgaaggcagc gataatcgcg aagaaaaccg tcgtaatcgt
    26521 cgccaggcac agcagcagac tgccgagacg cgtgagagcc gtcagcaagc tgaggtaacg
    26581 gaaaaagcac gtaccaccga cgagcagcaa gcgccgcgtc gtgaacgtag ccgccgccgt
    26641 aacgatgata aacgtcaggc gcaacaagaa gcgaaggcgc tgaatgttga agagcaatct
    26701 gttcaggaaa ccgaacagga agaacgtgta cgtccggttc agccgcgtcg taaacagcgt
    26761 cagctcaatc agaaagtgcg ttacgagcaa agcgtagccg aagaagcggt agtcgcaccg
    26821 gtggttgaag aaactgccgc tgccgaacca attgttcagg aagctccagc tccacgcaca
    26881 gaactggtga aagtcccgct gccagtcgta gcgcaaactg caccagaaca gcaagaagag
    26941 aacaatgctg ataaccgtga caacggtggc atgccgcgtc gttctcgccg ctcgcctcgt
    27001 cacctgcgcg tgagtggtca gcgtcgtcgt cgctatcgtg acgagcgtta tccaacccag
    27061 tcgccaatgc cgttgaccgt agcgtgcgcg tctccggaac tggcctctgg caaagtctgg
    27121 atccgctatc caattgtacg tccgcaagat gtacaagttg aagagcagcg cgaacaggaa
    27181 gaagtacaag tgcagccgat ggtgactgag gtccctgtcg ccgccgctgt cgaacctgtt
    27241 gttagcgcgc cagttgttga agaaatggct gaagtcgtag aagcccccgt accggttgcc
    27301 gaaccgcaac cggaagtggt tgaaacgacg catcctgaag tgattgccgc cgcggtaact
    27361 gaacagccgc aggtgattac cgagtctgat gttgccgtag cccaggaagt cgcagaacac
    27421 gcagaaccgg tggttgaacc gcaggaagag acggcagata ttgaagaagt tgccgaaact
    27481 gctgaggttg tggttgctga acctgaagtt gttgctcaac ctgccgcgcc agtcgtcgct
    27541 gaagtcgcag cagaagttga aacggtagcc gcggttgaac cagagatcac cgttgagcat
    27601 aaccacgcta ccgcgccaat gacgcgcgct ccggcaccgg aatatgttcc ggaggcaccg
    27661 cgtcacagtg actggcagcg ccctactttt gccttcgaag gtaaaggtgc cgcaggtggt
    27721 catacggcaa cgcatcatgc ctctgccgct cctgcgcgtc cgcaacctgt tgagtaataa
    27781 ttagctcaaa gtaatcaagc cctggtaact gccagggctt ttttatttca tgtttgaatc
    27841 aacatattaa aattcatcaa atggcatcct tgctaaccaa caatgcaaaa taggcaaaaa
    27901 aaatgcccgc cccggcgatc ccagagcggg cagacaaaac atacccagtt tcatgatatg
    27961 ttttaaagcg agcttatttg ttgatctgga agagcgacaa tccctgcata tcggtaaatg
    28021 ctttatacga tgcctgcaat gccgtttgct gcatgatgta agatgaaata gttgcattcc
    28081 agtccacatc aaccagatcg ctcatctgct gcgtttgccc taaagcgcga tcgctaccta
    28141 aggaatccag cgactccagt tcgttcagct gcgtgcctaa ttccgcgcga acggtaagga
    28201 cattattcag cgagtttttc agtccacggt tggttttatc caatgccgca gcggcgattt
    28261 ctttatccgc ttcgctatcc gcgaccggcg ttttcagggc tgcgatggcg ctatccagca
    28321 tggcaaaaag attggtttca gaagcgctac cgtctggttc cgctaccgcg ttactggtaa
    28381 tactgtcgaa aattttgtcg cccgtatgtc ctatcaccat cgaacgcgaa gcatcgacct
    28441 gttgcttaat actttctgtt ccgccgacat aatcaccgtt agcctcacta aacggcgcag
    28501 tctctgtttt ataaccggca aaaatgtagc gcccattacc gtcagtggtg ttcgccagat
    28561 tcagcaactg gtcacgaagc ccctgaatat ccgtagccag cgaggcccgg tcatcgtcac
    28621 tcaaggtgcc attgctggcg tagacaattt tttcctgagc attctggata gcagtggtga
    28681 cctggctaag tacactctct tccagtgaca ctttttgagt ggcgaaagta cgtgccagcg
    28741 cgtactggct gttttgcgcc tgtgcctggg agagaacaac agcttgtgat gcagcaatgg
    28801 gatcgtctga agggttaacg actcgcttac ccgtcgacat ctgctcgccg tacttcatcc
    28861 attctgcctg agaattggtg atgccgcgca tgttttgctg gtacatcatc tgggtactga
    28921 aacgcatttt atctcccctt agcgaatgtt aatcagcgca tcaaaaatcg cgtttgccgt
    28981 ctgcagaacc tgcgcattcg ccaggtaata ctgctgaaaa cgttgcagat ttccgtactc
    29041 ctcatcgaga ttgacaccgg aaatcgactg ctgctgattg gaaagctgcg tcaccacatt
    29101 accttgcgtg gtgctactgg ttttcaacgt cgcggtttta ttaccgatat cactcactaa
    29161 cgaggcataa acgtcgttaa aggatttcgc accgcccacc gttttactgt tgctttgcag
    29221 atccagcagg gcctgaccgt tgcggttgtc gctatcaccc gcatcttctt cgctcgccat
    29281 cgcgattttc gcttcgtcgg tgattaatac atccatgttg acgatggcgt cacttactgg
    29341 tttcagcgtg aagctgtcgt taacggcagg cgttcctgta aacgtcaact ccagaccatc
    29401 aaatgccact ttaccgttgg catccggcgt caccgtaaaa gtggtattgc tggcaaggcg
    29461 ggtgacctgc cactgattat tatcgaacga gattttgtaa tctgtcgcca gtaccgcgga
    29521 ggcatcagtt accgtggcac cgatcgcaac gtcacctttg tttttcgtgt tttgcagaac
    29581 cgcgggctta ccgatagcaa agaaatcttc accagcatcg ccgttggcat caaatccggc
    29641 tttgtgttgg ctgttgaaag cctcggcaaa tgccagcgcc agttgtccaa gcgtattacg
    29701 cgtctggtcc agatcctgag aacggaatgt cagaatgccg cccagcgacc cggtattcag
    29761 taatttctcc gggatctcaa tattgcctgc cgtcccatca acgtaagcga cagtcgtacg
    29821 agaagggtcg gcgctggaag gaactgccgc cagttgccgc gccgtacttc cctgaaccag
    29881 tgagtaacca ttggccatcg tgatgttata agtaccgcca tcctgaacgc tgacttctac
    29941 gccaacaatc tggtttaatt cgctcaccag ttgatcgcgt tgatccagca gattgttagg
    30001 tgacgcccct gcccccacgc ctgtcaggcg cgagatttga tcgttcaggc tggcaatttg
    30061 tttagcgtaa ttgttgatct gatcaacgct ggcacctatc gcgatattga cctgtttgtc
    30121 ctggtcgcgc agatattgat cggtggtttt aaactgattc accaaccctt ctgatttccc
    30181 aatcagcgcc tggcgcgctg ccgggtcttc cgcgttactc accagcgttt gcaggctggt
    30241 gaagaaatcc tgcatctgtg ttgccagcga agaggtactg gtggagagca tattgtcgat
    30301 tttcgacatc tgctcatagc gggcggtcag gccgctactt tgcgtctgcg cagcacgtaa
    30361 ctggttggta ataaacgcat catactcacg ctgcacacca gaaacgtaga cgccattgcc
    30421 aacccagccg ccagcgccca acgtgctatt ggcctgcgcc ataatagtgg tttggcgggt
    30481 atatccggca acgttatagc tggagatatt attacttgcc gtatttaacg ccgcctgggc
    30541 cgcgttcagt ccgctcatgg cgttattgat caagctggac atggaggttc cttataagcc
    30601 ttcaattaca gagtattatc ggcagcgacc cgccggactt gagttattca gaacagatta
    30661 tcaatgttca tactgtaggt tttgctcacc ttgtcgctta tcgatttcat ctgctgaatc
    30721 atgttggtga gtttacgggc atagtgagga tcggtggcat agcccgcgtc ctgtagggcc
    30781 tgcgccccct gctccgcact cgtggcggtc gtcacggcgg cgtagcgcgg gttacgcgtt
    30841 aacagcccaa cgtaatccga caaggcttcc agatacgagc tgtagacgcg aaacttcgct
    30901 tttactttct tcgcttcgcc gttttcatat tcagtcgtgg tgatttcagt aactggccct
    30961 ttccagttgc cagaggcttt gacaccaaac aggttatagc tcggctcgcc gttttcgcgg
    31021 cggatttgcc gttgccccca accagattcc agcgccgcct gagcgaggat caaatgatgt
    31081 ggcacaccgc tttgctggct tgccagttgg gcgggcagcg agagttgcgc gaggaatgct
    31141 ttactgtcac ccggcagcga atcatcgtag ttacgtggca cggccttttg caccagctgc
    31201 gaaagcgcct gattttgata acgcaccaca gtttcgagcg ggaatttcat cggtgctgct
    31261 ggcgtggact cctctggcaa tggttgttct ggcgtcatct gtttaaccat catctctgca
    31321 agccccagac ctttgcccgc cgtcatctgt tgggcaatct gctggtcata catactggta
    31381 tacaggcgag tgtgctcgct gctgaacagg ccatcttttg gtaaagcgtc gcgcatgctt
    31441 ttcaacatca tctgcacgaa catcccttcc acctgacggg ccaccggacg gatatttgcc
    31501 gccggatctt cgcccgcttt cgcctttagt tcgttgagtg attgcgcatc ccaggccgca
    31561 cttgccagta gtttgctgtc gctgatcatc agatgatttc cagttttgcc cgcagacatc
    31621 ccgcactttg cattgattgc agtatggaca tcagatccat cggcgtagcg cccagcgcat
    31681 tgagcgcgcg caccacgtta ttgaggctgg cgctggaacg tacgctttgc agcgaaccgc
    31741 cgctctggcg taaattgatc tgcgtttgtg gagtaaccac agtctgtcca ccaccaaacg
    31801 gtgtatctgg ctggctgaca ttggcctgac gattgactgt tactgagaga tttccctgcg
    31861 ctaccgcgca gctgtcgagg gtcacttcac gattcatcac caccgaaccg gtgcgcgagt
    31921 taatcactac tttagcgtcc tgcggggtga cattaacctg catattctgg atatctgcaa
    31981 ggaaacggac cagggaactg ttgccacttg gtacgcgcac ctgaatagtc cgcgcatcta
    32041 acgcggtggc gctgccatat ccacgcacgc ggttgatggt gtcagcgatt tgctgcgcca
    32101 tgctgaaatc ttcgtcgtta agttgcaaat taagggtatt cccgacgcca aactggctgg
    32161 gcaattcacg ttcaataacc gcaccattgg tgatccgtcc accgttcagt tggttcacct
    32221 gaacactgct accgccagcg gaggctcctg cgccgccaac cagaatattg ccctgcgcca
    32281 gcgcatacac ctgactgtca acgcccttaa gcggcgtcat caacaacgta cctccacgca
    32341 agcttttggc atttcccatg gaagaaacca ccacatcgat ggtttgcccc tgacgtccaa
    32401 acggaggaag tgacgctgtc accattaccg cagcgacgtt ttttagctgc atattggtgc
    32461 ccgtcggaac ggtaattccc agctgtgaga gcatgttatt aagcgtttgt gtggtaaacg
    32521 gcgtctgggt tgtctggtca ccggtgccat ccagccccac caccagacca tagccaatca
    32581 gtgagttttg ccttaccccc tgaacactgg tgagatcgcg aatacgctca gcctgagccg
    32641 ccgtcgtaac cagtagaaga attaatgcag agagaaattt aatcaccaca gcctcactta
    32701 cattggcgac aggttaagga agaaacgctg caaccagccc atattttgcg cttcgttaat
    32761 gtagccattg cctacgtatt caatgcgcgc atccgccacc tgagtagacg gtacggtatt
    32821 gctgccgctg atagtgcgtg gattaaccac gccagagaag cgaataaatt cggtaccctg
    32881 attaatggca atctgttttt cacccaccac atgcaggttg ccgttgacca gtacctggtc
    32941 aaccgtcacc gtcaacgtgc cgctaaaggt attgctggca ttggccccgc cctttccgtt
    33001 gaacgtgtta ccaccggagg cttcgacatc ggcacgagcg ttaccaaaca gcccctgcaa
    33061 atagcgtggc acagtatcaa agccaaaatt agttttaccg tcacggctgg cattcgcaga
    33121 ggagcttttg ctggcgctga cgttctcctg caacacgatg gtcagcgtat cgccaatatt
    33181 gcgtggtcga cgatcttcaa acagcggttg atagccatag ttaatcggct gagcagactg
    33241 gaaaatagaa ccgttggcga cgggcgtcgg accgggaacc ggttgtgcac tggtcgcccc
    33301 ctgcaccagc ggcgtggagg gtatccaggc gcaaccggtt agtgaaagca ccaacaagct
    33361 ggaaatggca taagtatgcg cagcgttttt ttgcatggct atcatcttca aaaatcagta
    33421 aaccggtgaa cctgccaccg gttaagcctt agagttgcgt cagtttttgc agcatctgat
    33481 cggtggtgga caccgcttta ctgttgattt cgtaagcgcg ttgcacctga atcatattga
    33541 ccagttcttc cgccacgttg acgttagacg tttcaacata cccttgatac agcagtcccg
    33601 cgccgttcag gcccggcgtg ctttcgttcg gtgcaccaga ggattgcgtt tcggtgtaga
    33661 ggttttcgcc aatgctctcc agcccggtgt cattcataaa ggtggtgaga ttgagctgcc
    33721 caacctgaac cggagctgcc tggccttgtt gggttacgct gaccacgcca tcacgaccga
    33781 tggtgatact taacgcattc gccggaatgg tgatcgctgg ctgcacctga aaaccaccag
    33841 ccgtcaccag ctgcccgttc tgatccacct ggaaagagcc gtcacgggta taggctgatg
    33901 aaccatctgg gaacatcacc tggaaaaagc cctgcccttt aatcgcgaca tctttgctgt
    33961 tgttggtctg cgacaggttt ccctggctgt gtaagcgttc agtggcgacc gggcgtaccc
    34021 ccgtgccgat ttgtaatccg gagggtaagg tggtttgttc ggaagactgt gcccccggct
    34081 ggcgaatggt ttgataaagc agatcttcaa acaccgcgcg ctgacgctta aaaccgttag
    34141 tactgacgtt tgccaggttg ttggcaatga cgtccatatt ggtttgctgg gcgtcaaggc
    34201 ccgttttggc gatccataat gaactgatca taaaatgtcc tgtagtggat tagtgacaag
    34261 cagagcagaa gcacgcgaca gtgtgaattt cccctcaccc taaccctctc cccaaagggg
    34321 cgagggaata gtccgcgcgc ttttattgca atgtcatcaa tcgttcaatt aactcatcga
    34381 cagcagttgg ttggcacggc ctacgttatc atcgacgctg ctgatcacct tcatctgcat
    34441 ttcaaaacgc cgcgcgctgg caatcatgtc gctcattgcc gcaacggcat tgacgttact
    34501 gccttccaga acccccgaca tcacacgcaa ggttggatct gcctgcagta ccggccctcg
    34561 cgtggcctgg gtttctgcgc ttaaacgaaa aatgccgtcg tcaccgcgct gcacttcgct
    34621 gcccgtggct ttcaccagtt tcagacgccc tactggcgca actgtatttg ccggatcgcc
    34681 cggattgagc gccgagattg tgccatcggc agcaatagtg atttccgctc cttccggcac
    34741 agcaattggc cccgcctcgc ctatcacctg atgcccctga attgtcagtt gcccggtggg
    34801 atcaacctga atgctgccat tacgcgtata cccttcgctg ccgtcagcgg tctgcacggc
    34861 cagccagcca tcctgctgca acgcaacgtc cagcgggcgc gaggtgtaat ccatcttgcc
    34921 gggcgtcatg tctgcacccg gtgttgacgc cgttaccagc gtgcgcgtgg gcagtgaaag
    34981 cccttccacc ggcactgcgc gcaaggcgtt taactgcgcg cgaaaaccgg gcgtagaggc
    35041 attggccaga ttactggcgg ttaccgcctg ttgattcagc gtctggctgg ctgctcccat
    35101 cgcagtataa attgcgtgat ccattgagct atcccgtcag cgattagcgt aagttaacca
    35161 gcgtgttgag gatctggtcc tgggttttga tggtctgggc gttagactga tagttacgct
    35221 gggcaacgat catattgacc agttctttac tgagatcgac gttggacgct tccagcgcac
    35281 cgttggtcag ggtgccaaag tttcccgtcc cggctgtccc caacagcgcc acgccagaag
    35341 attgcgtcgc agaccagacg ttgtcgcctt cggatgccag accttcgttg ttggcaaagt
    35401 tcgccagtac aatctgcccc agcagttggg tttgttcgtt ggaatagttg ccgacaaccg
    35461 taccgtcatc attgatttga taactcacca gatcgcccgg tttgtagccg ttctgggtgg
    35521 ttgccacaat attgttagcg ccggtatttt gctgcatgga gttgaggaag ctcagactaa
    35581 acgtggcggg ttctgcaccg ttaattgcgc cggttgcgat attattcgcc atcgcaccat
    35641 ccactaatgt gccattagca ttaaattcca gtgttgtcgc tgtcttcgca atgctgtttg
    35701 gatcactgct atcctgggtg tagacctgcc agttattatc cccggtcttc acaaagtaga
    35761 cgctcatgtc atgagcatta ccctgactgt cgaaaacagt caccgaacct tttttgttat
    35821 agctatccgc attgctggcg ctgaatggcg taacagtagg aagcggatcg ctggaattca
    35881 ggttgatctg catcgatgcc gtggtggtag ttttcgctgc catcagggta ttcgggatcg
    35941 aaatattagt cggattcgcc ccttgctgaa tagtcggcgg cgtaccggtt gccgggtaac
    36001 ccgtcagctg taaaccttgc atattcacca ggttacggtt ttcatccagc ttaaattgtc
    36061 cgttacggct gtagaacacc gaaccgttgc tgtctaccag acggaaaaaa ccgttctggc
    36121 tgatagcaac gtccagacct cgcccggtgt tggtggtcgt gccatcggta aagtcctgag
    36181 tgataccggc aacttttacc cccagtccca ctttcgaacc ggcaaacata tcggcaaaag
    36241 aggccgtgcc tgatttaaag ccgtaggtgg cggagttggc gatattgttg ccaataacat
    36301 cgaggttggt ggcggcagcg tttaatccgc taaccgcttg agaaaaggcc atgactgact
    36361 cctgaaatgt gaaggcttaa attatctgcc gtacttcgtc gagggtggtg gtgccgtaag
    36421 tgccgagatc cagcgtatta ccgttgttgc cgcggatcac accctgcacc agagcaaact
    36481 gcagcggctg ggcaaccagt tgtgtaccac cgttactggc gctaatcgct acattgtaag
    36541 aaccgttcgg cgcagtgctg ccatcagtca acgtaccgtc ccaggtgaaa ctgtgaactc
    36601 cggcggtcag ttcaccaata tcaatggtgc gcacaaccgc gccattttta tcggtgatgg
    36661 tggccgtaac tttgtctgcc gcctgttgca gctcaacacc aaacggcgtg gtcgtggtca
    36721 cagccccttc ttcactgccg gttcccgcaa gaacagtggt gccggggatc atcacgccgt
    36781 gaccgatcag gttactggcc tgtaacgact ggctgttatc aatctgtccg gaaatagatc
    36841 cgagcgtggt attgagtttt tcaatcccgc tgaccgtgct gatttgtgcc aattgcgacg
    36901 tcagctcgtt gttttccatt ggattggtcg ggtcctggtt tttcagctgc gccaccagca
    36961 aagtcagaaa actgctttgt aaatctgcgg cgttgctgcc cgtgagcgaa ctactgctgg
    37021 tggtactgac gccggtattt gtcggatcgg tggtggttac cgcaatggac atagctttct
    37081 cctttattga ccgagcgtaa gggttttcag catcatgctt ttcaccgtgt tgagcacttc
    37141 aacattggcc tgatagctgc gtgacgccga catggtgtta accatctctc cgacaacatc
    37201 aacgttcggc atttttacgt agccctttgc atctgccagc ggattacccg gttcataaac
    37261 cagtttgtcc ggggcctgac tttctataac atcggcaacc tttacgccgc ctgtcgcagc
    37321 acctggtgca gcgttaacct ggaataccac ctgttttgcc cgatatggct gtccatcggg
    37381 accggtcacg ctatcagcat tcgccagatt actggccgcc acgttcaggc gctgggactg
    37441 ggcagttaac gccgacccgg cgatatcaaa aatattcagc agtgccatcc gttaatttcc
    37501 gctctgtaaa acgttcatca tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg
    37561 gtattgcagg ctgttatcgg caaactgggt gcgttcgcga tccatatcga cggtattacc
    37621 gtcaagcgaa ggctggtccg gaatacggta ttgcagttct gcggtaggag gcgtcagcgc
    37681 ctgcgccgga atgtgttgcg ttgaggtcat cgtcagtgca accacactgg ttgcatcccg
    37741 tccacgttgc atgacttttt taagttcact ggcaaaatcg atatcgcgcg cctgataacc
    37801 aggggtatcg gcattggcga tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt
    37861 gagcgcctct tgttgaaaac gtaaggcggc gtcgagctta tcgagcatat ctcctccgca
    37921 ggtatcaaaa ttctgccatc agcttaaatg cctttacgcg cgcgttatcg gcggaataaa
    37981 cgcaaaatgg gtcgctattt atgccgttga tggtcattgc ggacaggtac aattcacgtt
    38041 gtagaaatgg ctgggggcga aaatgctgac aataaaacgt agcgtggcga tcatcgcgat
    38101 actgttcagt ccgttaagta cggcgagcaa tctcacatcg caattgcaca acttttttag
    38161 cgcccaactc gcgggggtaa gtgatgaggt tcgtgtttct attcgtacat cgcccaatct
    38221 actaccgcca tgcgagcagc cattgctttc gatgagcaat aattcccgcc tgtggggcaa
    38281 tgtgaatgtg ttggcacgct gcggtaacga caaacgatat ttacaggtta atgtgcaggc
    38341 cacaggaaat tatgtggttg ccgcgatgcc cattgtgcgg ggaggaaagc tggaagctgg
    38401 caatgtcaaa ctaaaacgcg gacggctgga taccctgcca ccgcgtacgg tgctggatat
    38461 caatcaactt gttgatgccg ttagcctgcg cgatctatca cccgatcaac ctatccagtt
    38521 aacccagttt cgccaggcat ggcgggtaaa agcgggacaa cgcgtcaacg tgatcgccag
    38581 cggtgatggg tttagcgcca acgcagaagg tcaggcgctg aacaatgcag ccgtcgcaca
    38641 gaatgcgcgg gtgcgcatgg tatcgggaca gatagtcagc ggcgttgttg atgcagatgg
    38701 gaatattctt ataaacctgt aagctgttaa agattacccg tcccttgccg ataaataagc
    38761 aacacatgat aaaagcgccc tcaatgagga ataaaccatg agtattgatc gcacttcgcc
    38821 tctgaagcct gtaagcaccg ttcaaccgcg cgaaaccact gatgcgccgg taacgaacag
    38881 ccgggcggca aaaacaaccg cctccaccag caccagtgtg acgttaagcg acgcgcaagc
    38941 aaaactgatg caacccggca gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact
    39001 ggcgattcgt aacggtgaac taaaaatgga caccggcaaa attgccgatg cgctgatcaa
    39061 cgaagcgcag caagacttgc agagtaactg atcgtatgac acgtcttgca gagatcctcg
    39121 accagatgtc cgctgtgctt aacgatctca aaacggtaat ggatcaagag cagcaacatc
    39181 tctctatggg gcagatcaac ggcagccagt tgcaatggat tacagaacaa aaaagctcac
    39241 tgctggcgac gctggattac ctcgaacagt tacgcaagaa agaacccaat acagcaaata
    39301 gcgttgatat tagtcaacgc tggcaggaaa ttactgtgaa aacgcagcaa ctacgccaaa
    39361 tgaatcaaca taacggctgg ttactggaag gacagattga gcgcaatcaa caggcgctgg
    39421 aaatgttgaa accgcatcag gaaccgacgc tatatggggc gaacggtcag acctcaacaa
    39481 cccatcgcgg cggtaaaaag atttcgatct gaatattatc tccggcctgc actgcaggcc
    39541 ggaatgcatt gttacaccgt ccggcgggca aattctttaa ctttgaagcc cagtaccgcc
    39601 agtgcagcga agtacgcggc aatccccgcc agcacgaccg ccattaaacg cagtaagcgc
    39661 cagggcatgg tacccaatga ccactccggc atgatatgta acatacctaa aagcacgcca
    39721 gacatcacca gtaccgccac caccagacgc aacagaaacg ccatccagcc gggttgcggg
    39781 gtaaagattt tctgcttacg caactgccag taaagcagcg aagcattcag acacgccgcc
    39841 agaccaatag aaagtgacag cccggcatgt ttcaacggac cgataaacgc caggttcatc
    39901 aattgcgtta aaatcagcgt aacgatggca attttcacgg gcgttttaat gtcctggcgg
    39961 gaataaaagc caggtgccaa cactttcact acaatcaggc caatcaaacc caccgagtag
    40021 gcaattaacg cccgctgggt catcagcgca tcaaacgcgg taaatttacc gtactggaac
    40081 agcgaaacgg ttaacggacc tgaaagaatg cccaacgcaa ccgcactcgg cagcgccaac
    40141 aggaaacaaa gacgcaaccc ccagtccatc aaacggttgt attcatcatg attgccactg
    40201 gcaaaacttt tcgacagcga cggcagcaaa atggtgccaa gcgccacgcc cagcacaccg
    40261 gacggaaact ccattaagcg gtcggcgtaa tacatccaag acaccgaacc ggaagcaaga
    40321 aacgaggcaa aaatggtgtt gatgattaag gagatctggc tcacagagac gccaaggatc
    40381 gccggtccca tctgtttcac cacgcgcatt gctccggcat cgtggaagtt aatgcgcggc
    40441 aggaccagca tgccgatctt cttcaggtgc ggtagctgat acaccagctg caggatgccg
    40501 ccgaccgtaa cagcccacgc cagcgccagc accggtgggt taaagtacgg tgcggcaaac
    40561 agcgcgaaac caatcatgct gatgttaagc agtgttggcg caaacgccgg aatcgagaag
    40621 cggttccacg tattcagaat cgctcccacc agcgacgcca gggagatcag caagatatag
    40681 ggaaaggtaa tctttagtag ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag
    40741 cctggcgcgg tcaccatgat cacccacggt gcggcgagca tgccagcgac cgtcacaacc
    40801 gccagcgcaa gtgtcagcag gccagaaaca taagagacaa agacccgcgt ggcgtcttca
    40861 ccctgcttac ttttatattc cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg
    40921 gcaaagatac ggcgtaacaa gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc
    40981 atccctgcgc caaagattct ggcgacaatt gcgtctcgtg cgaagccaag cacacgcgaa
    41041 aacatggtca tcgagctgac ggcggccagc gattttaata aattcatcgg tgttctaatc
    41101 cagacccacg ggcaaaacgc ctgcaaggca ggcgctgaaa gaggcgaata gtctacctgg
    41161 attatggtga attgctaccg ccagatgtta cagggtttat tcactcatcg catcgcgcca
    41221 gatcttgtca acgatacgtt gcgccagcac tgcctgttcg ccggcggttt gcggaactgt
    41281 ctgattttgc acacattcaa tgaagtgccg cgcacagccg acaaatccac gttgctcaag
    41341 cgtgctctgc caaccaggaa tcggtttatg cactacgccc tgcccgcgct cctcacgcca
    41401 ttcgcgcata tccgtaatgt cgatgagcgc accgtcagtc acggcctgca cggtttcacg
    41461 ctgacttccg gcacggcgat gcatacaggt ggtgatctgc aaaggaccag ccgaaaaatg
    41521 gtgctcggca aacagcattt cgccagcgtc gttagtcagt agcgtaccgc catccagaga
    41581 ggctttgcca cccgacaacc acagcgcggt atccaccaca tgcagataat catccagcaa
    41641 cgtgaaataa agatcgtgtg gcccgacgct attgctacga tgtttatcca ttcttaacga
    41701 ggctgcggtg gcgagttgcg tttttaactc tccgtagagt ggtgcgaaac gacggttaaa
    41761 accgaccatc aacgtcagtt ttttccgcgc cgccagttcc accagccgtt cagcatcgcg
    41821 cagattttct gccagcggtt tatcgacaca gacatgtacc cccgcattga gtaacgtact
    41881 gaccacgtca aagtggctgg cggtgctgga atgcacaaaa accgcatcgc aactggcggc
    41941 aaggctggat aacgaatcgg cataaggaat gcgccagctt tcacaaattg gcagggcttt
    42001 cgcgcgcgta ggcgaccagg ctccttgtaa cgtcccgtca gacgctgccg ccagcaccgg
    42061 taaccacgct ttttgcgcaa tgccacctaa tcccactacg ccgatacgta attttttcac
    42121 tttaatctcc cagatgggcc agcaacgaat caagacgctg tttcagttct gccacttcga
    42181 tttccagggc ttcgacgcgg gcctgtaaat caccatcaac cgcgttcgac atatccgtca
    42241 ccgccggctg atcttcaacc tcaccactga aaagatgcat gtagcggctt tcgcgtttac
    42301 ccggttcgcg ggccagacgc accacaaaag gaccatcttc gcgatttgcc agttgttcca
    42361 gcgtcgactc cacttccgcc atatcgctga attcatacat tcgcgcggcg cggctgcgca
    42421 gttcacctgg cgtctgggca ccacgcaata acaacgtggt gattaacgcc acttccgctg
    42481 cgctcagttt cagatcgcca aattctgaat tacaaaaacg ttgctcatat ttggtgaccc
    42541 gattaccaaa accgctcact gtgcgtagat aatgacgttt gaccagatta tccagctgtt
    42601 cctgcacttc agattcactc agattcatga ccggttcacg gttcgttttc tgattacagg
    42661 ccgtgactac accattgact gagagcgggt attgctccgg cgtggtcacc tgcttttcca
    42721 gcaagcaacc aatcacgcga gcttcaaggg cagtaagttg atatttcatg agattctcct
    42781 tagcggccgg gcgtccagtc tggggtagtt aatgccgtca gtacgtgatc gcgccattgt
    42841 ccatcaatca acagatagtc tttcgcatag ccttcttttt caaaacccag tcgcgccagt
    42901 aaatcaccgc tgcgtttatt gtgcggcata taattagcca taatgcgatg aatatgttgg
    42961 gtgcgctgca tataacgaat ggctgcggtc agggcttcaa acatgagtcc tttgccctgc
    43021 catttttgcc caatcgaata accgagatag caggcatgaa aagagccacg aacaacattg
    43081 gaaaaattgg caacgccaat aatctctttt tcgtccgggt cgaataagcc aaagtagaaa
    43141 gctgaacctt gtttatgaaa ttcgttaatc atccccagcc tggcctgcca gcctgatgga
    43201 taacagtggc tttcgtcgcg cactggctcc cagggcttga ggaaatggcg attctctgcg
    43261 taataatccg caagacgcca ggcatcacga tcatgcacca gacgcacgac cagtcggtct
    43321 gtggttaagc gcacttttgg cacgttactg cgatagccaa acattctata cctactcctt
    43381 cccgtaacgt ctttaatacg ctgtaagtat tactatacct gcgcaaccag accctgtgaa
    43441 aagtccaaaa tgccattttc tgcattattc acatatttga taaaaactct ctatcaaagc
    43501 gacgttttga taaaaaaata ttgtccccga agtgatggga aaagtcttag cggcaatgca
    43561 gaatagaaag tgctcatctt ttatttcccg ggaggggaaa tgtcccgcgt atcgcaggcg
    43621 aggaacctgg gtaaatattt cctgctcatc gataatatgc tggtcgtgct ggggttcttt
    43681 gttgtcttcc cgctgatctc tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg
    43741 gtcggtattg ctctcggtct acgccagttt attcagcaag gtctgggtat tttcggcggc
    43801 gcaattgccg accgctttgg tgccaaaccg atgattgtta ccggtatgct gatgcgcgcc
    43861 gccggattcg ccacaatggg tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg
    43921 ctctcgggac tcggcggcac gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta
    43981 atccgtccac aacaacgtgg tcgttttttc tcgctgttga tgatgcagga cagtgccggt
    44041 gcggtcattg gcgcattgtt ggggagctgg ctgttgcaat acgactttcg cctggtctgc
    44101 gccacagggg ccgttttgtt tgtgctgtgt gcggcgttca atgcgtggtt gttaccggca
    44161 tggaaactct ccaccgtacg cacgcccgtt cgcgaaggca tgacccgcgt gatgcgtgac
    44221 aagcgttttg tcacctatgt cctgacgctg gcgggttact acatgctggc tgtacaagtg
    44281 atgctgatgc tgccaattat ggtcaacgac gtggctggcg cgccctctgc cgttaaatgg
    44341 atgtatgcca ttgaagcgtg tctgtcgtta acgttgctct accctatcgc ccgctggagt
    44401 gaaagcattt tcgtctggaa caccggttga tggctgggct gttgataatg tcattaagca
    44461 tgatgccggt gggcatggtc agcggcctgc aacaactttt caccctgatt tgtctgtttt
    44521 atatcgggtc gatcattgcc gagcctgcgc gtgaaacctt aagtgcttcg ctggcggacg
    44581 caagagctcg cggcagctat atggggttta gccgtctggg tctggcgatt ggcggcgcta
    44641 ttggttatat cggtggtggc tggctgtttg acctgggcaa atcggcgcac cagccagagc
    44701 ttccgtggat gatgctggga attattggca tcttcacttt ccttgcgctg ggttggcagt
    44761 ttagtcagaa acgcgccgcg cgtcgtttgc ttgaacgcga cgcctgattt gatactcccc
    44821 cctgactcct ccatactgaa agtgaactga cagccaaatg gaggagtcac gtgaagctat
    44881 acatttacga tcactgccct tactgcctca aagcccgcat gattttcggc ctgaaaaata
    44941 tccccgtcga attacatgtt ctgctcaacg acgacgcaga aacacccacc cggatggtcg
    45001 gtcaaaaaca ggttcccatt ctgcaaaaag atgacagccg ctatatgcca gaaagcatgg
    45061 atatcgttca ctatgtcgat aaactcgacg gcaaaccgtt actgaccggc aaacgttccc
    45121 ctgccattga agagtggctg cgcaaggtca atggctacgc caacaaactg ctgttgccgc
    45181 gttttgccaa atcggcattt gatgagtttt ctactcccgc cgcgcgcaaa tatttcgtcg
    45241 acaagaaaga ggccagcgcg ggtaattttg ccgacctgct ggcccactct gacggtctga
    45301 ttaagaatat cagcgatgat ttgcgtgcgc tggacaaact gatcgtcaaa ccgaacgccg
    45361 tgaatggcga actttcggaa gatgatattc agctatttcc gctactgcgt aatctgacgc
    45421 tggtagccgg aatcaactgg ccaagccgcg ttgctgatta ccgcgataat atggcgaaac
    45481 agacacaaat caatttgtta tcatcaatgg cgatttaatc ctgacctgcc gcggcagcct
    45541 gtattcgcgg gttgccgccg ttttcagttt tttctgctgg cgagtttatc gctggtgaca
    45601 catgctgcgt tgtgtcagga tattgacgca gatttcatgt cgaggactac catgaacaag
    45661 tttttatttg ccgctgcatt gatcgtcagt ggcctgctcg ttggctgtaa tcaactcacc
    45721 caatacacca tcaccgaaca agaaattaac cagtcgcttg cgaaacataa taatttctca
    45781 aaagatatcg gtttacccgg cgtggctgac gcccatattg ttctgacaaa cttgaccagc
    45841 caaattggtc gcgaagagcc gaataaggtt accctaaccg gagacgccaa tctggacatg
    45901 aactccctgt tcggtagtca gaaagcgacc atgaaactga agctgaaagc gctgcccgtg
    45961 tttgataaag aaaaaggtgc gatcttcctg aaagagatgg aagtggtcga tgcgacggta
    46021 caaccggaaa aaatgcaaac ggtgatgcaa acgttgcttc cctatttgaa ccaggcatta
    46081 cgcaattact ttaaccagca acctgcttac gtcctgcgcg aagatggcag ccagggcgaa
    46141 gcaatggcga aaaaactggc gaaaggcatt gaagtgaagc caggcgaaat tgtcattccg
    46201 tttacggatt tatgacgagg gcgcattcgc gccctttatt tttcgtgcaa aggaaaacgt
    46261 ttccgcttat cctttgtgtc cggcaaaaac atcccttcag ccggagcata gagattaatg
    46321 actgcaccat cccaggtatt aaagatccgc cgcccagacg actggcacct tcacctccgc
    46381 gatggcgaca tgttaaaaac tgtcgtgccg tataccagcg aaatttatgg acgggctatt
    46441 gtaatgccca atctggctcc gcccgtgacc actgttgagg ctaccgtggc gtatcgccag
    46501 cgtattcttg atgccgtacc tgccgggcac aatttcaccc cattgatgac ctgttattta
    46561 acagattcgc tggatcctaa tgagctggag cgcggattta acgaaggcgt gttcaccgct
    46621 gcaaaacttt atccggcaaa cgcaaccact aactccagcc acggcgtgac gtcaattgac
    46681 gcaatcatgc cggtacttga gcgcatggaa aaaatcggta tgccgctact ggtgcatggt
    46741 gaagtgacac atgcagatat cgacattttt gatcgtgaag cgcgctttat agaaagcgtg
    46801 atggaacctc tgcgccagcg cctgactgcg ctgaaagtcg tttttgagca catcaccacc
    46861 aaagatgctg ccgactatgt ccgtgacgga aatgaacggc tggctgccac catcactccg
    46921 cagcatctga tgtttaaccg caaccatatg ctggttggtg gcgtgcgtcc gcacctgtat
    46981 tgtttaccca tcctcaaacg caatattcac caacaggcat tgcgtgaact ggtcgccagc
    47041 ggttttaatc gagtattcct cggtacggat tctgcgccgc atgcacgtca tcgcaaagag
    47101 agtagctgcg gctgcgcggg ctgcttcaat gccccaaccg cgctgggcag ttacgctacc
    47161 gtctttgaag agatgaatgc tctgcagcac tttgaagcat tctgttctgt aaacggcccg
    47221 cagttctacg ggttgccggt caacgacaca ttcatcgaac tggtacgtga agatcaacag
    47281 gttgctgaaa gcatcgcact gactgatgac accctggtgc cattcctcgc tggggaaacg
    47341 gtacgctggt ccgttaaaca ataaaaatcc tagctttctg ttgtcattag gttattttgc
    47401 ctgtataaat aaccagtata ttcaacaggg ggctattatg cgaattgaag tcaccatagc
    47461 gaaaacttct ccattgcctg ctggggctat tgacgccctg gctggcgaac tttcccgccg
    47521 tattcagtat gcgtttcctg ataatgaagg ccacgtatcg gtacgttatg ccgcagcgaa
    47581 taatttatcg gttattggcg caacaaaaga agataaacag cgcattagcg aaattctcca
    47641 ggaaacgtgg gaaagcgccg atgactggtt tgtcagcgaa taatatgcag tgatttttgt
    47701 ttttgccggg tcgccccggc tttttt
//
LOCUS       DAZKHC020000027        45756 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZKHC020000027 DAZKHC020000000
VERSION     DAZKHC020000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 45756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.027"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(5..838)
                     /gene="fdhD"
                     /locus_tag="ACHOSM_RS09090"
                     /old_locus_tag="pgaptmp_001818"
     CDS             complement(5..838)
                     /gene="fdhD"
                     /locus_tag="ACHOSM_RS09090"
                     /old_locus_tag="pgaptmp_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HGU4558362.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            1032..4082
                     /gene="fdnG"
                     /locus_tag="ACHOSM_RS09095"
                     /old_locus_tag="pgaptmp_001819"
     CDS             1032..4082
                     /gene="fdnG"
                     /locus_tag="ACHOSM_RS09095"
                     /old_locus_tag="pgaptmp_001819"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1617..1619,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU4558363.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVPVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4095..4997
                     /gene="fdoH"
                     /locus_tag="ACHOSM_RS09100"
                     /old_locus_tag="pgaptmp_001820"
     CDS             4095..4997
                     /gene="fdoH"
                     /locus_tag="ACHOSM_RS09100"
                     /old_locus_tag="pgaptmp_001820"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HGU4558364.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPHDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            4994..5629
                     /gene="fdoI"
                     /locus_tag="ACHOSM_RS09105"
                     /old_locus_tag="pgaptmp_001821"
     CDS             4994..5629
                     /gene="fdoI"
                     /locus_tag="ACHOSM_RS09105"
                     /old_locus_tag="pgaptmp_001821"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU4558365.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5626..6555
                     /gene="fdhE"
                     /locus_tag="ACHOSM_RS09110"
                     /old_locus_tag="pgaptmp_001822"
     CDS             5626..6555
                     /gene="fdhE"
                     /locus_tag="ACHOSM_RS09110"
                     /old_locus_tag="pgaptmp_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HGU4558366.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(6885..7127)
                     /gene="yiiF"
                     /locus_tag="ACHOSM_RS09115"
                     /old_locus_tag="pgaptmp_001823"
     CDS             complement(6885..7127)
                     /gene="yiiF"
                     /locus_tag="ACHOSM_RS09115"
                     /old_locus_tag="pgaptmp_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HGU4558367.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(7367..7564)
                     /gene="yiiE"
                     /locus_tag="ACHOSM_RS09120"
                     /old_locus_tag="pgaptmp_001824"
     CDS             complement(7367..7564)
                     /gene="yiiE"
                     /locus_tag="ACHOSM_RS09120"
                     /old_locus_tag="pgaptmp_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGU4558368.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDD"
     gene            complement(7941..8057)
                     /locus_tag="ACHOSM_RS09125"
                     /old_locus_tag="pgaptmp_001825"
                     /pseudo
     CDS             complement(7941..8057)
                     /locus_tag="ACHOSM_RS09125"
                     /old_locus_tag="pgaptmp_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8421..9362)
                     /gene="fabY"
                     /locus_tag="ACHOSM_RS09130"
                     /old_locus_tag="pgaptmp_001826"
                     /gene_synonym="yiiD"
     CDS             complement(8421..9362)
                     /gene="fabY"
                     /locus_tag="ACHOSM_RS09130"
                     /old_locus_tag="pgaptmp_001826"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HGU4558369.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(9407..9844)
                     /gene="dtd"
                     /locus_tag="ACHOSM_RS09135"
                     /old_locus_tag="pgaptmp_001827"
     CDS             complement(9407..9844)
                     /gene="dtd"
                     /locus_tag="ACHOSM_RS09135"
                     /old_locus_tag="pgaptmp_001827"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGU4558370.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(9841..10713)
                     /gene="yihY"
                     /locus_tag="ACHOSM_RS09140"
                     /old_locus_tag="pgaptmp_001828"
     CDS             complement(9841..10713)
                     /gene="yihY"
                     /locus_tag="ACHOSM_RS09140"
                     /old_locus_tag="pgaptmp_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HGU4558371.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(10707..11306)
                     /gene="yihX"
                     /locus_tag="ACHOSM_RS09145"
                     /old_locus_tag="pgaptmp_001829"
     CDS             complement(10707..11306)
                     /gene="yihX"
                     /locus_tag="ACHOSM_RS09145"
                     /old_locus_tag="pgaptmp_001829"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HGU4558372.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(11405..12190)
                     /gene="csqR"
                     /locus_tag="ACHOSM_RS09150"
                     /old_locus_tag="pgaptmp_001830"
     CDS             complement(11405..12190)
                     /gene="csqR"
                     /locus_tag="ACHOSM_RS09150"
                     /old_locus_tag="pgaptmp_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4558373.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(12224..13120)
                     /gene="yihV"
                     /locus_tag="ACHOSM_RS09155"
                     /old_locus_tag="pgaptmp_001831"
     CDS             complement(12224..13120)
                     /gene="yihV"
                     /locus_tag="ACHOSM_RS09155"
                     /old_locus_tag="pgaptmp_001831"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HGU4558374.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            13288..14184
                     /gene="yihU"
                     /locus_tag="ACHOSM_RS09160"
                     /old_locus_tag="pgaptmp_001832"
     CDS             13288..14184
                     /gene="yihU"
                     /locus_tag="ACHOSM_RS09160"
                     /old_locus_tag="pgaptmp_001832"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HGU4558375.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            14208..15086
                     /gene="yihT"
                     /locus_tag="ACHOSM_RS09165"
                     /old_locus_tag="pgaptmp_001833"
     CDS             14208..15086
                     /gene="yihT"
                     /locus_tag="ACHOSM_RS09165"
                     /old_locus_tag="pgaptmp_001833"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HGU4558376.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            15103..16207
                     /locus_tag="ACHOSM_RS09170"
                     /old_locus_tag="pgaptmp_001834"
                     /pseudo
     CDS             15103..16207
                     /locus_tag="ACHOSM_RS09170"
                     /old_locus_tag="pgaptmp_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
     gene            16345..17247
                     /gene="yihR"
                     /locus_tag="ACHOSM_RS09175"
                     /old_locus_tag="pgaptmp_001835"
     CDS             16345..17247
                     /gene="yihR"
                     /locus_tag="ACHOSM_RS09175"
                     /old_locus_tag="pgaptmp_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HGU4558377.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            17446..19482
                     /gene="yihQ"
                     /locus_tag="ACHOSM_RS09180"
                     /old_locus_tag="pgaptmp_001836"
     CDS             17446..19482
                     /gene="yihQ"
                     /locus_tag="ACHOSM_RS09180"
                     /old_locus_tag="pgaptmp_001836"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HGU4558378.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFNAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            19528..20913
                     /gene="yihP"
                     /locus_tag="ACHOSM_RS09185"
                     /old_locus_tag="pgaptmp_001837"
     CDS             19528..20913
                     /gene="yihP"
                     /locus_tag="ACHOSM_RS09185"
                     /old_locus_tag="pgaptmp_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4558379.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDITGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLTAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSEFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            20956..22359
                     /gene="yihO"
                     /locus_tag="ACHOSM_RS09190"
                     /old_locus_tag="pgaptmp_001838"
     CDS             20956..22359
                     /gene="yihO"
                     /locus_tag="ACHOSM_RS09190"
                     /old_locus_tag="pgaptmp_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4558380.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            22427..23119
                     /gene="ompL"
                     /locus_tag="ACHOSM_RS09195"
                     /old_locus_tag="pgaptmp_001839"
     CDS             22427..23119
                     /gene="ompL"
                     /locus_tag="ACHOSM_RS09195"
                     /old_locus_tag="pgaptmp_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HGU4558381.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(23210..24475)
                     /gene="yihN"
                     /locus_tag="ACHOSM_RS09200"
                     /old_locus_tag="pgaptmp_001840"
     CDS             complement(23210..24475)
                     /gene="yihN"
                     /locus_tag="ACHOSM_RS09200"
                     /old_locus_tag="pgaptmp_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4558382.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(24577..25557)
                     /gene="yihM"
                     /locus_tag="ACHOSM_RS09205"
                     /old_locus_tag="pgaptmp_001841"
     CDS             complement(24577..25557)
                     /gene="yihM"
                     /locus_tag="ACHOSM_RS09205"
                     /old_locus_tag="pgaptmp_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGU4558383.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(25565..26275)
                     /gene="yihL"
                     /locus_tag="ACHOSM_RS09210"
                     /old_locus_tag="pgaptmp_001842"
     CDS             complement(25565..26275)
                     /gene="yihL"
                     /locus_tag="ACHOSM_RS09210"
                     /old_locus_tag="pgaptmp_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4558384.1"
                     /translation="MTGNQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KSRTSDIPQTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(26492..28315)
                     /gene="typA"
                     /locus_tag="ACHOSM_RS09215"
                     /old_locus_tag="pgaptmp_001843"
     CDS             complement(26492..28315)
                     /gene="typA"
                     /locus_tag="ACHOSM_RS09215"
                     /old_locus_tag="pgaptmp_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HGU4558385.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            28688..30097
                     /gene="glnA"
                     /locus_tag="ACHOSM_RS09220"
                     /old_locus_tag="pgaptmp_001844"
     CDS             28688..30097
                     /gene="glnA"
                     /locus_tag="ACHOSM_RS09220"
                     /old_locus_tag="pgaptmp_001844"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HGU4558386.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALFYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            30271..31320
                     /gene="glnL"
                     /locus_tag="ACHOSM_RS09225"
                     /old_locus_tag="pgaptmp_001845"
     CDS             30271..31320
                     /gene="glnL"
                     /locus_tag="ACHOSM_RS09225"
                     /old_locus_tag="pgaptmp_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HGU4558387.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            31332..32741
                     /gene="glnG"
                     /locus_tag="ACHOSM_RS09230"
                     /old_locus_tag="pgaptmp_001846"
     CDS             31332..32741
                     /gene="glnG"
                     /locus_tag="ACHOSM_RS09230"
                     /old_locus_tag="pgaptmp_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HGU4558388.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            32853..32963
                     /gene="yshB"
                     /locus_tag="ACHOSM_RS09235"
                     /old_locus_tag="pgaptmp_001847"
     CDS             32853..32963
                     /gene="yshB"
                     /locus_tag="ACHOSM_RS09235"
                     /old_locus_tag="pgaptmp_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HGU4558389.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(33153..34526)
                     /gene="hemN"
                     /locus_tag="ACHOSM_RS09240"
                     /old_locus_tag="pgaptmp_001848"
     CDS             complement(33153..34526)
                     /gene="hemN"
                     /locus_tag="ACHOSM_RS09240"
                     /old_locus_tag="pgaptmp_001848"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HGU4558390.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(34715..35224)
                     /gene="yihI"
                     /locus_tag="ACHOSM_RS09245"
                     /old_locus_tag="pgaptmp_001849"
     CDS             complement(34715..35224)
                     /gene="yihI"
                     /locus_tag="ACHOSM_RS09245"
                     /old_locus_tag="pgaptmp_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HGU4558391.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(35343..35509)
                     /locus_tag="ACHOSM_RS09250"
                     /old_locus_tag="pgaptmp_001850"
                     /pseudo
     CDS             complement(35343..35509)
                     /locus_tag="ACHOSM_RS09250"
                     /old_locus_tag="pgaptmp_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            35806..36438
                     /gene="yihA"
                     /locus_tag="ACHOSM_RS09255"
                     /old_locus_tag="pgaptmp_001851"
     CDS             35806..36438
                     /gene="yihA"
                     /locus_tag="ACHOSM_RS09255"
                     /old_locus_tag="pgaptmp_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGU4558392.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(36820..39606)
                     /gene="polA"
                     /locus_tag="ACHOSM_RS09260"
                     /old_locus_tag="pgaptmp_001852"
     CDS             complement(36820..39606)
                     /gene="polA"
                     /locus_tag="ACHOSM_RS09260"
                     /old_locus_tag="pgaptmp_001852"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGU4558393.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGNELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            39970..40902
                     /gene="yihG"
                     /locus_tag="ACHOSM_RS09265"
                     /old_locus_tag="pgaptmp_001853"
     CDS             39970..40902
                     /gene="yihG"
                     /locus_tag="ACHOSM_RS09265"
                     /old_locus_tag="pgaptmp_001853"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGU4558394.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLNQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(40943..42373)
                     /gene="yihF"
                     /locus_tag="ACHOSM_RS09270"
                     /old_locus_tag="pgaptmp_001854"
     CDS             complement(40943..42373)
                     /gene="yihF"
                     /locus_tag="ACHOSM_RS09270"
                     /old_locus_tag="pgaptmp_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGU4558395.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(42528..43154)
                     /gene="dsbA"
                     /locus_tag="ACHOSM_RS09275"
                     /old_locus_tag="pgaptmp_001855"
     CDS             complement(42528..43154)
                     /gene="dsbA"
                     /locus_tag="ACHOSM_RS09275"
                     /old_locus_tag="pgaptmp_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HGU4558396.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(43171..44157)
                     /gene="srkA"
                     /locus_tag="ACHOSM_RS09280"
                     /old_locus_tag="pgaptmp_001856"
     CDS             complement(43171..44157)
                     /gene="srkA"
                     /locus_tag="ACHOSM_RS09280"
                     /old_locus_tag="pgaptmp_001856"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HGU4558397.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(44234..44503)
                     /gene="yihD"
                     /locus_tag="ACHOSM_RS09285"
                     /old_locus_tag="pgaptmp_001857"
     CDS             complement(44234..44503)
                     /gene="yihD"
                     /locus_tag="ACHOSM_RS09285"
                     /old_locus_tag="pgaptmp_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HGU4558398.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            44573..45157
                     /gene="mobA"
                     /locus_tag="ACHOSM_RS09290"
                     /old_locus_tag="pgaptmp_001858"
     CDS             44573..45157
                     /gene="mobA"
                     /locus_tag="ACHOSM_RS09290"
                     /old_locus_tag="pgaptmp_001858"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HGU4558399.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     AQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDTFVNVNTPEELAQWQEKR"
     gene            45139..45666
                     /gene="mobB"
                     /locus_tag="ACHOSM_RS09295"
                     /old_locus_tag="pgaptmp_001859"
     CDS             45139..45666
                     /gene="mobB"
                     /locus_tag="ACHOSM_RS09295"
                     /old_locus_tag="pgaptmp_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HGU4558400.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT        11267 a        11683 c        11252 g        11554 t
ORIGIN      
        1 ttaattaatt gctcaaacgc tgcggatggg tataaaccgt tgcccgaccc ggtttacaaa
       61 aacctaccag tgtcagatta cagcgctcag ccacttctac agcaagcgtg gtcgcggcag
      121 acaccgcaaa caaaatctct acgccgcaca tcgccgactt ttgcaccatt tcataactgg
      181 cacgactgga aaccagtacc gcaccttgct gccagctttc cccttcttgt gaccggcggc
      241 ctaacagttt gtccagcgcc acatggcgac ccacgtcttc atgcccgccg accagttcgc
      301 cagatggcaa catccaggca gcggcgtgag tacaaccagt cagttgcccc actggctgaa
      361 aatcgttgag atgacgtaat gcatcatcca gtttgttgag atcaaacgtc tgggtgaacg
      421 gtagcggctg caccggtttt ccgatgtcat taagttgctc cacgccgcat acgccacagc
      481 ccgtacgtcc cgccagcgcc cggcggcgct ccttcaaccc cataaagcgg cggctggaaa
      541 gctcaatttg tacttcaaga ccattacagg aaggaacgac atccatgccg aagatatcgc
      601 gcggactttc tattatcccc tcggaaagcg aaaaaccgag cgcaaagtac tcaaggtctt
      661 tgggcgacgc catcatcacc acatgcgaaa tgccgttgta gaccagcgca acgggaactt
      721 cttccgcgac ctcatctaac cgtgggtgtt gtaaatcatc acgccgccat aactcaattt
      781 gacgaacacc tgtgatattt gtcacatttt cgatttcttt ttgctgtgtt tttttcactt
      841 tctcttaacc agataagaac acacacacca acattatggt attctgttac aaacccttcc
      901 tggatggagg gaaattgagc caattctgga cctttgcggc cccttccgca aagaaaaata
      961 actcccactc cctgcacacg cagcaagcga atgtaaatgg gacgtgacaa tgtcgaaaca
     1021 aggagcaatc catgcaggtc agcagaaggc agttctttaa gatctgcgct ggcggtatgg
     1081 caggcaccac ggcagcggca ctgggttttg cacccagcgt agcgctcgcg gaaacccggc
     1141 agtataaact gctgcgcacc cgcgaaaccc gtaatacctg cacctattgt tccgtaggct
     1201 gtgggctgtt gatgtacagc ctcggtgacg gagcaaaaaa cgccaaagca tctatcttcc
     1261 atatcgaagg cgatccggat cacccggtca accgcggtgc actttgtccg aaaggcgctg
     1321 gcctggtgga tttcatccac tccgaaagcc gtctgaagtt tccggaatac cgtgcgccag
     1381 gttctgataa atggcagcaa atcagttggg aagaggcgtt tgatcgcatc gccaaactga
     1441 tgaaagaaga ccgcgatgct aactacattg cgcaaaacgc cgaaggcgtg cctgttaacc
     1501 gctggctctc caccgggatg ctgtgtgctt ccgcgtcgag taacgaaacc ggctatttaa
     1561 cgcaaaaatt ctcccgcgcg ctgggtatgc tcgcggtcga caaccaggcg cgtgtctgac
     1621 acggaccaac ggtagcaagt cttgctccaa catttggtcg cggtgcgatg accaaccact
     1681 gggtcgacat caagaacgcc aacctcgtcg tggtgatggg cggtaacgcc gctgaagctc
     1741 acccggtcgg gttccgctgg gcgatggaag ccaaaattca caacggcgcg aagctgattg
     1801 tgatcgatcc tcgctttacg cgtacggcgg cggtagctga ctactatgcc cctattcgtt
     1861 ccggtactga cattgctttc ctgtcaggcg tattgctgta cctgctgaac aatgaaaaat
     1921 tcaaccgcga atacaccgaa gcctatacca acgccagcct gatagtgcgt gaggattacg
     1981 gctttgaaga tggcctgttc accggctacg acgcggaaaa acgcaagtac gataaatcct
     2041 cctggactta tgaactggac gaaaacggct tcgccaaacg cgataccacg ctgcaacatc
     2101 cgcgctgcgt gtggaacttg ctgaaacagc acgtttcccg ctacacgcca gatgtggttg
     2161 aaaacatctg tggtacgcca aaagacgcgt tcctgaaagt ctgcgaatac atcgcagaaa
     2221 ccagtgctca cgataaaact gcctcgttcc tgtatgccct cggctggacg caacactccg
     2281 ttggtgcgca aaacattcgt acgatggcga tgatccagct gctgctcggc aatatgggga
     2341 tggccggcgg cggcgttaac gccctgcgcg gtcactccaa tattcagggg ctgacggacc
     2401 tggggctgct gtcgcagagt ctgccaggtt acatgacgct gccaagcgag aagcagaccg
     2461 atctgcaaac ctaccttacc gccaacacgc caaaaccgct gctggaaggc caggtaaact
     2521 actggggcaa ctacccgaaa ttcttcgtct ctatgatgaa ggccttcttt ggtgataaag
     2581 cgacggcgga aaatagctgg ggctttgact ggttgccgaa gtgggataaa ggctacgacg
     2641 tcctgcaata cttcgagatg atgaaagaag gcaaggtcaa tggctatatc tgccagggct
     2701 ttaaccctgt tgcctcattc ccgaacaaaa acaaagtgat cggttgtctg tcgaaactga
     2761 agttcctcgt caccatcgac ccgctgaaca ctgaaacctc taacttctgg cagaaccacg
     2821 gtgagctgaa cgaagttgac tcgtcgaaga tccagaccga agtgttccgt ctgccatcga
     2881 cctgcttcgc agaagagaac ggttcaatcg ttaactcagg tcgctggttg cagtggcact
     2941 ggaaaggtgc ggacgccccg gggattgcgc tgactgatgg cgagatcctc tccggtatct
     3001 tcctgcgctt gcgcaagatg tatgccgaac agggtggcgc gaacccggac caggtgctga
     3061 acatgacctg gaactacgcc attccgcatg agccgaagtc ggaagaagta gcgatggaga
     3121 gcaacggtaa ggcgctggcc gatattaccg atccggcaac cggtgcggtt atcgtcaaga
     3181 aaggccaaca acttagctcg ttcgcccaat tgcgcgatga cggtacaacc tcctgtggct
     3241 gctggatttt cgccggtagc tggacgccgg aaggcaacca gatggcgcgt cgtgataacg
     3301 ccgatccgtc tggcctcggt aacacgctgg gctgggcatg ggcatggccg cttaaccgcc
     3361 gcattctgta taaccgcgcc tccgcagatc cgcagggtaa cccgtgggat ccgaagcgtc
     3421 agttgctgaa atgggacggc actaagtgga ccggctggga tattccggac tacagcgcag
     3481 cgcctccggg tagcggcgtc gggccgttta tcatgcagca ggaaggcatg gggcgtctgt
     3541 ttgccctcga taagatggcg gaaggtccgt tcccggaaca ctacgagccg tttgaaacgc
     3601 cgctgggaac taacccgctg catccaaacg ttatctcgaa cccggctgca cgtatcttta
     3661 aagacgacgc cgaagcattg ggtaaagccg ataagttccc gtatgtcgga accacctatc
     3721 gtctgaccga gcatttccac tactggacca aacacgcgct gttgaacgcg attttgcaac
     3781 cagagcagtt tgtggaaatc ggggagtcgc tggcgaataa acttggcatt gcccagggcg
     3841 ataccgtgaa agtctcctcc aaccgtggct atatcaaggc caaagcggtg gtgaccaaac
     3901 gtattcgtac gctgaaagcg aacggcaaag atatcgatac catcggtatt ccgattcact
     3961 ggggttatga aggcgtagct aaaaaaggct ttattgccaa tacgttaacg ccattcgttg
     4021 gtgatgcgaa cacgcagacg ccggagttta agtccttcct tgtgaatgtg gaaaaggtgt
     4081 aacggagacg acatatggct tatcaatctc aagatatcat tcgtcgttcc gcgactaacg
     4141 gtctgacccc cgcgcctcag gcgcgggact tccaggaaga agtggcgaaa ctcatcgacg
     4201 tcaccacctg tatcggctgt aaagcctgtc aggtggcgtg ttcagagtgg aacgacattc
     4261 gcgataccgt cggcaataat attggggtgt acgacaaccc ccatgattta agcgccaaat
     4321 cgtggacggt gatgcgcttc tcggaagtgg agcagaacga caaactggaa tggctgatcc
     4381 gcaaagacgg ctgtatgcac tgttccgatc ccggctgcct gaaagcgtgc ccggcggaag
     4441 gggcaattat tcagtatgcc aacggtatcg tcgacttcca gtccgagcag tgcattggct
     4501 gcggttattg cattgcgggc tgtccgttcg acattccgcg cctcaacccg gaagacaacc
     4561 gcgtctacaa atgtacgctg tgcgttgacc gcgtggtggt tgggcaagaa ccagcctgcg
     4621 tgaagacctg cccaacgggc gcgattcact tcggtacgaa agagtcgatg aaaacgctgg
     4681 cgagcgagcg cgtggcggag ctgaaaaccc gtggttacga caatgcgggc ctgtacgatc
     4741 cggcaggcgt cggtggtaca cacgtcatgt acgtgctgca ccatgctgac aagccaaatc
     4801 tgtatcatgg cttgccggag aacccggaaa tcagcgaaac cgtgaaattc tggaaaggca
     4861 tctggaaacc gctcgcggct gttggctttg cggctacctt tgcggccagt atcttccact
     4921 acgtgggtgt cggtccgaac cgtgcggatg aggaagagaa taatctgcac gaagagaaag
     4981 acgaggagcg caaatgaaac gacgtgacac catcgtgcgc tacaccgcgc cggaacgtat
     5041 caaccactgg atcaccgcct tctgcttcat cctggcggcg gtgagcgggc tgggcttttt
     5101 gttcccgtcc ttcaactggt tgatgcaaat catgggcaca ccgcagctgg cgcgaattct
     5161 gcacccgttt gtcggcgtgg ttatgtttgc ctcgttcatc atcatgtttt tccgttactg
     5221 gcatcacaac ctaatcaatc gggatgatat cttttgggcg aagaatattc gtaagatcgt
     5281 cgtcaacgag gaagtaggtg acaccgggcg ttataacttc ggtcagaaat gcgttttctg
     5341 ggcggcgatt attttcctgg ttctgctgct ggtgagcggc gtgattatct ggcgtcctta
     5401 ttttgcgcct gctttctcaa tcccggtgat ccgattcgcg ttaatgctgc attcatttgc
     5461 cgcagtggcg ttaattgtgg ttatcatggt gcatatctac gccgcccttt gggtgaaagg
     5521 cacgattacc gccatggtgg aaggatgggt taccagcgcc tgggcgaaga aacatcaccc
     5581 gcgctggtac cgtgaggtcc gcaagacaac ggaaaagaaa gctgaatgag tattcgcata
     5641 atcccgcaag atgagctggg ttcgagcgag aaacgtacgg cggatatgat tccgccgtta
     5701 ttgttccctc ggctcaagaa tttatacaac cgccgcgccg agcgtctgcg cgagctggca
     5761 gaaaataatc cgctgggtga ttacctgcgc tttgctgcgc ttatcgccca cgcccaggaa
     5821 gtggtgctgt acgaccatcc gctggagatg gatctgaccg cgcgcattaa agaagccagc
     5881 gcacagggca agcccccgct ggatattcac gttctgccgc gtgataagca ctggcaaaag
     5941 ctgctgatgg cgctgattgc tgagctgaaa cctgaaatga gcggcccggc gctggcagtg
     6001 attgagaatc tggagaaggc atcgactcag gagctggaag atatggccag cgcactgttt
     6061 gcctctgatt tctcatccgt cagcagcgat aaagcgccgt ttatctgggc tgcactgtcg
     6121 ctctactggg cgcagatggc caatctgatc cccggcaaag cccgcgctga atacggcgag
     6181 caacgtcaat attgcccggt ttgtggttct atgccggtgt ccagcatggt gcaaattggc
     6241 accactcagg gtctgcgtta cctgcactgc aacctgtgtg aaaccgaatg gcacgtagtg
     6301 cgcgtaaaat gcagcaactg cgagcagagc ggcaaactgc attactggtc gctggatgac
     6361 gaacaggccg cgattaaagc cgaaagctgc gatgactgtg gcacttacct gaagattctt
     6421 tatcaggaaa aagaaccgaa agttgaagcc gtggcagatg acctcgcctc tctggtactg
     6481 gacgcgcgaa tggagcaaga aggctacgcc cgcagttcca ttaacccgtt cctgtttccg
     6541 ggtgaagggg agtaatctct gacgttttcc gggtggcgct ggtcgcccgg attacagctc
     6601 atagggtgtg acgacactat ctctcgtatt ccgcgttacc cattcaagat catgccgcaa
     6661 ttgtctgttt gcctcgttct tccctccaac acatcgataa ggttatgggt atgtcactga
     6721 tgacaagcgt atgattgcct gatgcgctgc gcttatcagg cctaccaata aacttcaata
     6781 tattgaattt gcatgacttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac
     6841 aacgcgcacg ttgtcagcaa tctgattgac atcatttttt cccattacca ttcctcgcgc
     6901 agcttacgct gatattcgac accatcttcc tcacaccctt gccagatgcc aggaacactg
     6961 gttcttgctg tttgcgattg atttatcagg tattgatcta ccgcttccct taataggtcc
     7021 gcacgaggaa gattacgctg cacctcgaga tcatcaagtt gcttaatcac ctcattcgat
     7081 aaatcgagta aaattctgcc catatctata cctgccagag agttcataaa tatctcgcca
     7141 atatcatttt tgaatctatg gagagaaaag tacccttgcc gaatctttaa agaaagctca
     7201 tttacgcatc attttttatt tttgcgaggc gttttccaga tacgatgtaa cagttgcagt
     7261 tattataagt tcaagtaaaa cagccttgta ttaataccag aataagggca tctgtttttt
     7321 tatattcaag aatgaaaaat ttttttcatt ccttatgctc cttacactaa tcatcaacac
     7381 caactacctc ccccttttgc ctgcctccct ttgctttacg caccggaaat tgctgccgct
     7441 ccagatattg ctcaaccgct tcgcgtaaaa ttgcagatcg tgaaacctta cgccagcccc
     7501 gaagtttgtt cagccgttta attgcctctt ctgataaatg aagaaaaacc gtattcatcg
     7561 ccattaccac caccttacct atattactca ttaatgtatg tgcgaaaatt acgccggaaa
     7621 atcatttaaa aaaagcggat ttttgcatgt tgttttgttt ttgcgaggcg ttttccagaa
     7681 ataataagag tgttgcagtt gtttcattta ttactcaaaa atggtttagt tatcgcccag
     7741 gatgaattat gggtgctatg aatatattat attcagagat atttatttgc ataatctgaa
     7801 tggtgtagcc gaaaaacgtc agcatcctaa cagcaccagg atttaaggtg aaattaatct
     7861 ttcatcacag accggtcagt actgccacca accggtcttt acttgctcat cgttataatt
     7921 ttgttggtaa tagttcggga aagttgtttt tctaaattaa caacagaata actttcttgt
     7981 ttaatgagat taatattggc aaccattata gcctccttcc cacggtttaa gagcaaacat
     8041 acaacataat gattcataac atacagagca gtaaatgaat gatactgaca atgatgccag
     8101 cccattatct ttgcataatg tttgtgagct atagatttta aaaatattta tgaaaaaagt
     8161 atggcaccag agggggtttt gactatcaaa aatcgctcaa acctgaacta agatgatgag
     8221 ttaagcatta tctgcgataa ttgccactca gctctacaaa taatatttaa atgctcctga
     8281 acagtgatgt agcaagacac gtatggtgcc aacgaagaat aaaaatttat agagttctta
     8341 ttcaatatta ccggcataga cagggaatga tgtaaatgac cggaatgaat gagagaggca
     8401 gcaccgcccc tctccatcag ctactcttct tcgttcccgc cctcttcata cgggccaaat
     8461 ggcttcgcgg gcagaacgat atacgtgcct tcaaacactg cacccggcgt ctcgtcgcca
     8521 aagatttcaa cttgcatctg cacccgtgct tttcgtccgc gcgccagacg gtcgagatcg
     8581 ccgcttaagg caccaaggtc ggctaccgca tgaggtttac cgctaatcgg cttgctgtag
     8641 cggatatgcg catccgcaag aataatcgtt ccgccgaggt ggcgttcacg cagcatcagc
     8701 cagataagtc cccaaccggt gagcgtcgcc agcgagaata aactcccggc aaacaacgta
     8761 tggtgcggat tctgattacc ggtttctggc atggtggtga taaatttttg cccggtatat
     8821 tgctgaatgc gcacgcccat tttttcgctt aacgggatat gttcgtacca cgcctgttgc
     8881 agctgcgcgc accagtcacc gcgatgcaga atgtcatcca gagtggcaac gggtttaatc
     8941 atcaaaaaat ggcgaatcgg cgtggtagtt ggggtggtga tctccccctg attaatgaat
     9001 cccagcttgg cgaaaaactc caccgcgtct tcacgggcgc tacaggtcac gcgcttaacg
     9061 ccttcctgac gcgccaccga ctccagggtc atcgccatca gcgtgcctaa ccctttgtcc
     9121 tgcacgtccg ggtgaacggc cataaagcga atggacgctt cattgtcggc attaatatac
     9181 agtcggccta ccgccaccag attaccctgc tcgtcgacga ccatctggtg atgcgccatc
     9241 gcatcccacg cgtcgcgttc cgaacctttt ggttgatgca ggggcttacg caacatttcc
     9301 cagcgaaact gatagtaacg ctctaactct tcttctgttt gtggaacccg aaggtgatac
     9361 atagcgatac tctctcttgt ttcccgtgac aaccctggaa gctggctcat acctgcaacc
     9421 agaatgttac ggggccatca ttgaccagcg atacctgcat atcggcagcg aagcgtcctg
     9481 tttgcgtgtt catctcttgc tgacggcagc gttcgacgaa atagtcatat aacgcctctg
     9541 cgcgatccgg tgatgcgcct ttggagaaac ttgggcgcat cccccgttcg gtatctgcgg
     9601 cgagggtaaa ctgggaaacc accagcacac tgccgcccgc ctgttgcacg ttgaggttca
     9661 tcttgccttc ggcatcgcta aagatgcggt agccgagcac acgctcgcac agacggtttg
     9721 ctttctgttc gtcgtcatcc ttttcgacac ccaataacac caaaagtccc gcgccaattt
     9781 cgcccgtcac ttctccctcc acggtgacgc tggcacgggt tacgcgttga attaatgcaa
     9841 tcatggttcg tcgtcttctt cttgttcagc tgcttgtttt agtttgcggt attccccgag
     9901 agtgacagta atttccgcgc caagcaagac gatacaccat gtccagtaga cccaaacaaa
     9961 gagaatgggg atcaccgcca acacgccgta aatgagctga tatgacggga acatggtgat
    10021 ataaagcgcg aaacctttct ttcctgcttc gaacaggagc gcggcgacaa acgcgccgac
    10081 aatcgcgtcg cggttaggta cgcggatggt aggaacaatg ctgtacagca accagaagga
    10141 gatccacgac aacagcagcg gaaaaatacg cagcacgtta tcgatgacag tattgagatc
    10201 gctcgcccag cgcagggaga gcaaatagga actgatcgcc agactggccc ctgccagcag
    10261 cggccccagc gttaaaatca tccagtacac ggcgaacgag taaatttttg gtcgcgctcg
    10321 tttactgcgc cagatggtat tcaacgcgct atcgatggag tacatcagca ataacgcggt
    10381 gacgatcagc ccgcacgccc caacggcggt catcttgttg gaattggcaa caaattgttc
    10441 gatataccgc tgaataacat cgccagtagc aggcagaaag ttggcaaaaa taaagtgacg
    10501 taactgaatg ctgacgtcgg aaaacatggg aaaagcggcg aaaagcgcaa aaacaacggc
    10561 aaccagcggc actaatgaga gcaacgacac ataggcaagg ttacctgcca gggttgtcat
    10621 gttgtcctca tcaatgcgtt gccagagtag ttttagccag gcccatagtg gacgggtacg
    10681 gtgcctggct ttgtcctgaa tggtttttag cataacacct tcgcgaaata gtccgggatg
    10741 gtggttttat ctttcaccag aatactggta atgcccagct gattggctcc ttctatatta
    10801 tcggcgttat cgtcgaaaaa gaccgtatcg ctgggtgaaa aaccttccgc ctgcaaaaca
    10861 tgctggtaaa ttcgtgcttc aggtttgcgc atccccagat cttgcgacag atagatatgg
    10921 tcagcagcat cacgaatttc cgggtattct tccggccaga aggtggtatg caggcggttg
    10981 gtattggaaa gcaccaccac gcgatgcccc tgctcacgca gtttatgcat gatggcgatc
    11041 acttccgggc gcagcgcaac aaacaccgcc tgccagccgt gagagaactg ctcgtagctt
    11101 agcggtagag ccatctcatg acacagcgcc tctgcgaacg cttcgtcgct aatttcccca
    11161 cgctcatgct gatgaaacgc ctcccccata tgaaaactct tcttaagcga tgccagcgga
    11221 atacgcgtta aatcgctcca ggctcccagc acacggttaa agtcgatatc gacaatcaca
    11281 ttacctaaat caaagatata gagcattttt gcctcctttc gcgccacaag aaattaactg
    11341 tagcgggaaa ggatgggttt ggctatgtgg caaggtatga agccaggtta acggtcacaa
    11401 aaatctacgc gtcttcctgg ggaagaatga tttcaatttg gctgtcttgt aagcgtgatt
    11461 ttagcgcagc gggcggcagc tcgtcggtaa agagcgcagt gacctgtgcc acgttaccaa
    11521 tttcaaccgc cgccgaagca tgatacttag tgtgatcggc gaccagcaga atatttctcg
    11581 cgtgcgccat catcgttttc accacgttag cttcgtttac atcaaactcc atcaacgcgc
    11641 catcgctctc aatcgcccca acgcttgtta ccagataatc agcacgaaaa tcagccacaa
    11701 aggacgccgc tgaagggcca atgatcccgc tattatgaga gcgcaacgta ccgccgggca
    11761 ccatcacttc aaagcgcggg ttgtggtaaa gaatatgcgc cacacgcagg ctgttggtga
    11821 ttatccgcaa atgattatgg ttaagtaacg cccgggcaac atgctcaaca gtcgtaccaa
    11881 tggtgataaa tattgttgaa ccatcaggaa tatagtctgc caccgcttcg gcaatcgctt
    11941 ttttttcctc ggtttgcgaa acctcacgct gctcgaacgc cgtattaacg acgctggaag
    12001 cccgacccgc gccaccgtga tggcgcgtaa tcaggccttg ctcgcttaat ttacgaatat
    12061 cccggcggac cgtctgcgtg gagacatcca gcagatttgc cagctcatca atattcatat
    12121 acccacgctc ggcgatcagc atgaggagtt ggtcgtgccg cgggttaccg gttagttcgg
    12181 taaggctcat gaaaattcct cgaaaaacca tcaccctggc attttataca aaaagtgaca
    12241 aaaaagatcg ggtttgatca cagtcaggga tcccggctcg tccaccggga cgtgtgcatt
    12301 ttaacgccgc tacaccgctg gcgaagcgga ctgactccgc taaatcccca cttgttgcca
    12361 gcgccaccgc caaagcgccg tgaaaaacat cacccgcacc tgtggtatct accacatcaa
    12421 ctttgaaggc cggttgatgc tgacgcccac cattttccag ccagtcgcag cctgcgctac
    12481 cctgggtcac atagacatga ccatttgtga gcgtttgtgc ctgttttagc gcactggcca
    12541 tctctttcac gcccgttaag cgcgccagac ccggttctga aaaggccgcg tgatcgctta
    12601 atgccaccag ctcactgata tcctgcggcg taatgtcccc gtccagaacg gtcatcacac
    12661 ccgcctgacg ggccagggtg aaggcttttt tagcgccgtc gtgccagcgt acatctgcca
    12721 gcacaacatc ccactgagag aaatcaattt cctccaacca ctctgcgtca ggcagcagat
    12781 ccgggctggg gtagttaatg attatccgct cgcctttggt atccaccatg atggcggatt
    12841 gcgaagattt cgcctggtta taccgtttgg tgtaacgggt gttaacgccc caggattcca
    12901 gctctgccag caggctgttg cccgtgtcgt catcacctac gcgaccaata aaatcgacct
    12961 gcgcccccag ccttgccgcc gcaaccgctg ccgtcgccgc tggcccgcca ccaacttccg
    13021 tataatttct cgccacgtat ttaccgctct ccgtcggtaa cccttccacg taatagatgc
    13081 gatccatcac ggttatacct acacaagcaa cacgaatcat ggtcattcct taaacatttt
    13141 gggatgcgtt cattttaatt tcaccaattg tttaaaaagt gacggctgtc aattttttga
    13201 cgataaatta caaatacgat caaaaacaga caatcaaagc atgtcataaa tgacaaaaag
    13261 tgacatcact gtattcagga gagggttatg gcagcaatcg cgtttatcgg tttaggacaa
    13321 atgggttcgc caatggcgag caatttattg cagcaagggc atcaacttcg cgtctttgat
    13381 gtgaacgccg aagctgtgcg gcatctggta gacaaaggtg cgactcccgc cgccaacccg
    13441 gcgcaggccg ctaaagatgc cgaatttatc attaccatgt tgccgaatgg cgatctggtg
    13501 cgcaacgtgt tgttcggtga aaacggcgtt tgcgaaggct tatctaccga tgcgctggtc
    13561 attgatatgt ccaccatcca tccgctgcaa accgataaat tgattgccga tatgcaagcc
    13621 aaaggcttca gcatgatgga tgttccggta ggccgtactt ctgcaaatgc cattaccggt
    13681 actctgttac tgctggctgg cggcaccgct gaacaagttg aacgtgccac accgatcctg
    13741 atggcgatgg gcagtgagtt gatcaacgct ggcggtccgg gcatggggat ccgcgttaag
    13801 ctcatcaaca actacatgag catcgcgctc aatgcgcttt cggcagaagc cgccgttttg
    13861 tgcgaagccc tgaatcttcc cttcgatgtt gccgtcaaag tgatgagcgg taccgccgcc
    13921 ggtaaaggtc acttcaccac ttcctggcca aacaaagtcc tcagcggaga tctttctccc
    13981 gccttcatga tcgatcttgc ccataaggat cttggcatcg cccttgatgt cgccaaccag
    14041 ctgcatgtgc caatgccgct gggggccgcc tcacgggagg tttatagcca ggcgcgcgca
    14101 gcgggtcgcg gtcgccagga ctggtccgcc attctggaac aggtccgtgt cagtgccggg
    14161 atgactgcca aagtaaaaat gtaacgactg gataaaggaa taaatgaatg aataagtaca
    14221 ccatcaacga cattacgcgc gcatcgggcg gttttgccat gctggcggtc gatcagcgcg
    14281 aagccatgcg catgatgttt gccgcggctg gggcacccgc tccggtagcc gatagcgttt
    14341 taactgattt caaagttaac gctgcaaagg ccctctcgcc ttatgcctcg gcgattctgg
    14401 tagatcaaca attctgctac cgccaggtgg ttgagcaaaa cgcgattgcc aaaagttgcg
    14461 ccatgattgt cgccgccgat gagttcattc ctggcaacgg tattccggtc gatagcgtgg
    14521 ttattgaccg caaaatcaat ccgctacaga tcaaacaaga cggtggcaaa gccttaaaac
    14581 tgctggtgct gtggcgtagt gatgaagatg cgcagcaacg tctggatatg gtgaaagagt
    14641 tcaacgaact gtgccactca cacggtctgg taagcatcat tgagccagtc gtccgcccac
    14701 cgcgtcgtgg cgataaattc gatcgcgaac aagcgatcat cgatgccgcc aaagagctgg
    14761 gcgacagtgg cgctgacctc tacaaagttg aaatgccgct ttatggcaaa ggtccgcaac
    14821 aagagcttct ctgtgcttca caacgtctga atgaccatat caatatgcca tgggtgatcc
    14881 tctcttccgg tgtcgacgaa aaactgttcc cgcgtgccgt acgcgtggca atgacggcgg
    14941 gcgcatcggg attcctggca ggccgtgcag tctgggcatc ggtcgtcggt ttaccagaca
    15001 acgagctgat gctgcgtgac gtttgcgcac cgaaattaca acaacttggc gatatcgtcg
    15061 acgaaatgat ggctaaacgc cgctaagatg aggataaaag gaatgaaatg gtttaacacc
    15121 ctaagccaca accgttggct ggaacaggaa accgaccgca tctttgattt tggtaaaaat
    15181 tccgtagtgc cgactggttt tggctggtta ggcaataaag ggcaaatcaa agaagagatg
    15241 ggcacccatc tgtggatcac cgctcgtatg ttgcacgttt attccgttgc tgcggcgatg
    15301 ggtcgacctg gcgcttactc gttggttgat cacggtatca aagccatgaa cggcgcactg
    15361 cgtgataaaa aatatggcgg ctggtatgcc tgcgtgaatg acgagggcgt ggtggatgcc
    15421 tccaaacagg gctatcaaca cttctttgct ctgctgggtg ccgccagcgc cgtcacaacg
    15481 ggccacccgg aagcgcgcaa gctgctcgat tacaccattg aaattatcga gaaatatttc
    15541 tggagcgaag aagagcagat gtgcttggaa tcctgggacg aagccttcag caaaaccgaa
    15601 gcattatcgc gttaacgaac acttcgatac ccagtggaat ccgctgccgg attacaacaa
    15661 agataacccg gcgcatcgct tccgcgcgtt cggaggtaca ccaggccact ggatcgaatg
    15721 gggccgttta atgctgcaca tccatgctgc cctggaagcc cgttgcgaac aaccaccagc
    15781 atggctgcta gaagatgcca aaggtctgtt taacgccacc gtgcgcgatg cctgggcacc
    15841 cgatggcgcg gacgggattg tttataccgt tgactgggaa ggaaaaccgg tggtccgcga
    15901 acgtgtacgt tggcctatcg tcgaagcaat gggtacggcc tacgcgctct acaccgtcac
    15961 tggcgatcgc cagtatgaaa cctggtatca aacatggtgg gagtactgca ttaagtacct
    16021 gatggactat gaaaatggtt cctggtggca ggagctggat gcggacaata aggtcaccac
    16081 caaagtctgg gacggcaaac aggatattta tcacctgctg cattgcctgg tgatcccgcg
    16141 tatcccgtta gcgcccggca tggctccagc ggttgcagcg ggtctgctgg atattaatgc
    16201 gaaataactg atctgaaaat agggtgcggg ttttcccctc accctaaccc tctccccaaa
    16261 ggggcgaggg gaccgtccgg tgcagttttt gggtttgaca tcagcctcac gggaggctgt
    16321 atgtcgttaa ttaaggtgcc ttgtatgcaa atcaccaata tgcattgcag tggacagacc
    16381 gtttcacttg ccgcggggga ttatcacgcg accattgtga ccgtcggtgc tggtctggct
    16441 gaattaacct ttcagggttg tcatttagtc atcccgcaca aacccgaaga gatgccgctg
    16501 gcgcatctgg gtaaagtgct tattccgtgg ccgaaccgga tcgccaacgg ctgttaccgc
    16561 tatcaggggc aggaatatca gttacccatt aatgaacata gttcaaaagc cgctattcac
    16621 gggctgcttg cctggcgaga ctggcaaatt agtgaattaa ccgccaccag cgttaccctg
    16681 acagcttttc tgccgcccag ttacggctat ccgtttatgc tggcttcaca agttgtttat
    16741 tcgctcaacg cacacacggg tctttccgtt gagatcgcaa gccagaatat cggcactgtc
    16801 gcagcgcctt atggcgtggg tattcatcct tatttaacct gcaacttaac atctgttgat
    16861 gaatatctgt tccagcttcc ggcaaaccag gtttatgccg tcgatgagca cgcgaatccg
    16921 acaacgcttc accacgttga tgagctggat ttaaatttca cgcaggcgaa aaaaatagcc
    16981 gcgacaaaaa tcgaccacac ctttaaaacc gctaatgatt tgtgggaaat gaccattacc
    17041 catccacagc aggcgctttc ggtaagcctt tgttccgatc aaccctgggt gcaggtttat
    17101 agcggtgaaa aattacagcg tcagggactg gcagttgaac ctatgagttg cccgcctaac
    17161 gcctttaatt caggtatcga cttattatta ctggaaccag gaaaaccgca ccgcctgttt
    17221 tttaatattt acggacagag aaaataactt gtattgatta gcagtcaaaa tcattgaaga
    17281 acatttcact cgctaaaggc aacattatgt atcttcaatt tattaagtta aaaacagctc
    17341 agcttctgta aagtttttat atatcagttt tatgagaatc attttacata agcctgtaat
    17401 aacaggaagg caacaatctc gttctcaact ctatggagat tagttatgga tacgccacgt
    17461 ccacagttat tagattttca atttcatcag aataacgaca gttttaccct acattttcaa
    17521 caacgtctta ttttaaccca tagcaaagat aatccttgtt tatggattgg ctcaggtata
    17581 gcggatatcg atatgttccg cggtaatttc agcattaaag ataaactaca ggagaaaatt
    17641 gcgcttaccg acgccatcgt cagccagtca ccggatggtt ggttaattca tttcagccgt
    17701 ggttctgaca ttagcgccac gctgaatatc tctgccgacg atcagggccg tttattgctg
    17761 gaactacaaa acgacaacct taaccacaac cgtatctggc tgcgccttgc cgctcaacca
    17821 gaggaccata tctacggctg cggcgaacag ttttcctact tcgatctgcg cggcaaaccg
    17881 ttcccgctat ggaccagtga acaaggcgtt ggtcgcaaca aacaaaccta tgtcacctgg
    17941 caggccgact gcaaagaaaa tgcgggcggc gactattact ggactttctt cccacagcct
    18001 acgtttgtca gcacacagaa gtattactgc catgttgata acagttgcta tatgaacttc
    18061 gactttaatg ccccggaata ccatgaactg gcgctgtggg aagacaaagc aacgctgcgt
    18121 tttgaatgtg ctgacacata catttccctg ctggaaaaat taaccgccct gctgggacgc
    18181 cagccagaac tgcccgactg gatttatgac ggagtaacgc tcggcattca gggcgggacg
    18241 gaagtgtgcc agaagaaact ggacaccatg cgtaacgcgg gcgtgaaggt caacggcatc
    18301 tgggcgcagg actggtccgg tattcgtatg acctcttttg gcaaacgcgt gatgtggaac
    18361 tggaagtgga acagcgaaaa ctacccgcaa ctggattcac gcattaagca gtggaatcag
    18421 gagggcgtgc agttcctggc ctatatcaac ccgtatgttg ccagcgataa agatctctgc
    18481 gaagaagcgg cacaacacgg ctatctagca aaagatgcct ctggcggtga ctatctggtg
    18541 gagtttggcg agttttacgg cggcgttgtc gatctcacta atccagaagc ctacgcctgg
    18601 ttcaaggaag tgatcaaaaa gaacatgatt gaactcggct gcggcggctg gatggctgac
    18661 ttcggcgagt atctgcccac cgacacgtac ctgcataacg gcgtcagtgc cgaaattatg
    18721 cataacgcct ggcctgcgct gtgggcgaag tgtaactacg aagcccttga agaaacgggc
    18781 aagctcggcg agatcctttt ctttatgcgc gccggttcta ccggtagcca gaaatactcc
    18841 accatgatgt gggcgggcga ccagaacgtc gactggagtc tcgacgatgg cctggcgtcg
    18901 gttgtcccgg cggcgctgtc gctggcaatg accggacatg gcctgcacca cagcgacatt
    18961 ggcggttaca ccaccctgtt tgagatgaag cgcagcaaag agctgctgct gcgctggtgc
    19021 gatttcagcg ccttcacgcc gatgatgcgc acccacgaag gtaaccgtcc tggcgacaac
    19081 tggcagtttg acggcgacgc agaaactatc gcccattttg cccgtatgac ctccgtcttc
    19141 accaccctga aaccttacct gaaagaggct gtcgcgctga atgcgaagtc cggcctgccg
    19201 gttatgcgcc cgctgttcct gcattacgaa gacgatgcgc acacttacac cctgaaatat
    19261 cagtacctgt taggtcgcga cattctggtc gctccggtgc atgaagaagg ccgtagcgac
    19321 tggacgctct atctgccgga ggataactgg gtccacgcct ggacgggtga agcgttccgg
    19381 ggcggggaag ttaccgttaa tgcgcccatc ggcaagccgc cggtctttta tcgcgccgat
    19441 agcgaatggg cggcactgtt cgcgtcgtta aaaagcatct aatcacatcc gcccgtgggg
    19501 aaactcatgg gcaaagggag aacatcgatg agtcacatca caacggaaga tccagcaact
    19561 ttacgcctgc cctttaaaga gaaactctct tacggtattg gcgacctggc ctctaacatc
    19621 ctgctggata tcggtacgct ttatcttttg aagttttata ccgacgttct ggggctgcca
    19681 ggcacctatg gcggcattat ctttttgatt tcaaaattct ttactgcgtt taccgatatg
    19741 ggtaccggca ttatgttgga ttcccgacgc aagatcggtc caaaaggtaa gttccgtcct
    19801 tttattctgt atgcgtcatt cccggtcacc ttactggcga tcgccaactt tgtcggcaca
    19861 ccgtttgata tcaccggtaa aacggtgatg gccactattc tgtttatgct ttacggactg
    19921 tttttcagca tgatgaactg ctcctacggc gcaatggtcc cggcaatcac caaaaacccc
    19981 aacgaacgcg cctcactgac ggcatggcgt cagggcggcg ctacgctggg cctgctgctg
    20041 tgcacggtgg gattcgtgcc agttatgaat cttatcgaag gtaatcagaa acttggctat
    20101 atcttcgccg ccacgctgtt ttcactgttc ggcctgctgt ttatgtggat ctgctactca
    20161 ggcgtgaaag agcgttatgt cgaaacccaa ccaaccaatc cggcgcaaaa gcctggcctg
    20221 ttgcaatctt tccgcgcaat tgccggtaac cgcccactgt tcattctgtg tattgccaac
    20281 ctctgcacct taggggcgtt taacgtcaag ctcgccattc aggtctatta cacccagtac
    20341 gtacttaacg atcccatcct gttgtcgtat atgggatttt tcagcatggg ctgtattttc
    20401 atcggcgtgt tcctgatgcc tggcgcagtc agacgttttg gtaagaagaa ggtctatatc
    20461 ggcggcctgc tgatttgggt gctgggcgat ctgctcaact atttcttcgg cggcggctcg
    20521 gtcagctttg tggcgttctc ctgcctggcg ttcttcggct cagagtttgt taacagcctg
    20581 aactgggcgc tggtttccga caccgtcgag tacggcgagt ggcgcaccgg cgtgcgttcg
    20641 gaaggcacgg tctacaccgg cttcaccttc tttcgcaaag tgtctcaggc gctggctggt
    20701 ttcttccccg gctggatgct gacgcaaatt ggctatgtgc cgaacgtcgc acaggctgac
    20761 cacaccattg aagggttacg ccagttgatc ttcatctacc caagcgcact ggcggtagtc
    20821 accattgtgg cgatgggttg cttctacagc ctgaacgaga agatgtatgt ccgcattgtg
    20881 gaagagatag aagcccgtaa acgcacggcg taataataat aaacgacgcc ctgcggggcg
    20941 ttataaggag tgattatgtc tgaccataat ccactgacat taaaactgaa tctgcgggaa
    21001 aaaatcgcct atggtatggg cgacgtcggt tcgaatttaa tgctctgcat cggtactctg
    21061 tatctcctca aattttatac cgatgaatta gggatgcctg cttactatgg cgggattatc
    21121 tttctggtgg cgaagttttt taccgcgttt accgatatgc tcaccggatt tttactcgac
    21181 tcgcggaaaa atattgggcc taaaggcaaa tttcgtccct ttattttata tgcagcagtt
    21241 ccggcggcgt taattgcaac gctccagttt atcgccacca ccttttgttt gccggttaaa
    21301 acgacgattg ccaccgcgct gtttatgatg tttggacttt catacagtct gatgaactgc
    21361 tcgtatggtg cgatgatccc ggcaattacc aaaaacccga atgagcgcgc gcagctcgcg
    21421 gcttaccgcc agggtggtgc aaccataggg ctattgattt gtaccgtggc gtttattccg
    21481 ttgcagtcgc ttttttctga ctcaaccgtc ggttatgcct gtgcggcact tatgttctcc
    21541 attggcggct ttatttttat gatgctgtgc tacagaggcg tcaaagagca ttatgtggac
    21601 acagcgccaa ccggacataa agccagtatt ctcaaatctt tttgcgcgat atttcgtaat
    21661 ccaccattgc tggttttatg cattgctaac ctgtgtactc tggcggcatt taatatcaaa
    21721 ctggcgattc aggtctatta cacccagtat gtgctgaatg atattaattt attgtcgtgg
    21781 atgggattct tcagtatggg atgcatcctc gtcggcgtat tactggtgcc agtaactgta
    21841 aaatgttttg gtaaaaaaca ggtttattta gctggcatgg tgctatgggc ggtgggtgat
    21901 atactgaatt atttctgggg aagtaactct ttcactttcg tcatgttctc ttgtgtcgcc
    21961 ttttttggca cggcctttgt aaacagcctg aactgggcac tggtaccaga taccgtagat
    22021 tacggtgaat ggaaaaccgg tattcgtgcc gaaggttctg tttataccgg ttataccttc
    22081 tttcgtaaaa tttctgccgc acttgctggc ttcttgccag gcattatgct gacgcaaatt
    22141 ggttatgttc ccaacatcgc gcaaagcgat gcgacattac agggtttgcg tcagctcatt
    22201 tttatctggc cttgtgcact ggcaattatt gctgcattga cgatgggatt cttttacaca
    22261 ctcaatgaaa aacggtttgc attgattatt gaggaaatca accaacgcaa aaataaagag
    22321 atggcgacag aagaaaaaac ggcttccgta acgttataaa tgtcaatagc cgctatttcc
    22381 atcctggtgg gtggcggcct ccctacgttt aaaaaatgga cttattatga aaaagattaa
    22441 tgcaataatt ttattatctt ctctgacctc tgcctcggta tttgctggcg catacgtcga
    22501 aaacagggag gcatataatc ttgcctccga tcagggcgag gttatgctac gtgtgggtta
    22561 taacttcgat atgggcgcgg gtattatgtt aaccaatacc tacacctttc agcgagaaga
    22621 tgaactaaaa catggctata atgaaattga aggttggtat cctttattca aaccaaccga
    22681 taaattaacc atccagcccg gtggcttaat taatgataag agtatcggtt cgggtggtgc
    22741 agtgtatctg gacgtcaact ataaatttgt accatggttt aatctgacag tacgaaatcg
    22801 ctataaccat aataactata gttcaacaga tttgagcggg gaactggata ataatgacac
    22861 ctatgaaatt ggcacctact ggaattttaa aatcaccgat aaattttcct atacatttga
    22921 gccacattat ttcatacgcg taaatgactt taatagcagc aatggaaaag atcatcactg
    22981 ggaaatcact aataccttcc gttatcgcat taatgaacac tggctgcctt atttcgaatt
    23041 acgttggtta gatcgtaatg tcgaaccgta ccaccgcgag caaaaccaga ttcgtatcgg
    23101 gacgaagtat ttcttctgat gtgaaactgc cggatgcggc gtaaacgcct tatccggcct
    23161 acggttgata cggttttcgt tggccggatt aagcacatcc ggccattaat caagcttcag
    23221 ccgccagcat ctctttctta cgttggctaa ttcgcttaac cagcattacg gaaaccaccg
    23281 caccacagaa tgcgaagcag gccataatgc caaacacgat tttgtagcca ataatccccg
    23341 gatttaaatc cagaatgtag ccatacagac tgaagcagaa catcgccgga gcgtaaccaa
    23401 taaagctacc caacgccatc gccgcgcctg ttttattttc agcaattttt gcttcgccga
    23461 taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc agcgtacatg
    23521 ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt acgagcgcag
    23581 cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg atcttatctg
    23641 aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta atgatgccgt
    23701 aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga atgaagaatg
    23761 tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc cagatagttt
    23821 tgtctttcag caccgaggtc atactggtgt tttgcgatgc cccgtcttct tttttcacct
    23881 caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg cctacggcaa
    23941 tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca ctgccaaacc
    24001 aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca cgccctgttt
    24061 cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt accgacttga
    24121 gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag acaaagagga
    24181 tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc gcaatgagtg
    24241 agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac agaaaaaagc
    24301 ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca ttggtcaaat
    24361 ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat ttataaattg
    24421 taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg agcataacat
    24481 cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac cattcatata
    24541 gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa gatggtttgc
    24601 ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa ttgcatcttc
    24661 tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg tttcactcat
    24721 ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg ccggcgcata
    24781 gtaatcaact tcttcttcca ggccatatgc cgtcacctgc ggctcatcat caccaaggca
    24841 gataaggtgc gttaataacg ctttgaacgg catatcaccc tgacctgaaa tacaggcttt
    24901 atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga cctgggtaat
    24961 atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga tcatgttcgc
    25021 aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg acaccaactc
    25081 atgacttttt aaatcttcat gctgctcgat cgtaaaagtc aggccgctgt cctgatacgt
    25141 ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca aattaccttc
    25201 ataacgtgga taaaaacgta cggacgatgc cccagttttc aacgcgatgg cgacggcttc
    25261 gtcaatagat gccttatctg aggcgctggt ttcaatatga atatcaaggt tcagacggcg
    25321 ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag aaaaacgctc
    25381 gccatccaga acatggattt tgacgccacg tagattattt tcacttgcga tatcaagtaa
    25441 atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag catgtaaata
    25501 aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg tcaccataaa
    25561 acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca atatctatct
    25621 ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct ggcatacccg
    25681 cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg aaaatcaatt
    25741 tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat gagtggtttt
    25801 caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg tgatagaaaa
    25861 ctttatgtcc ttccagcgcc ccccagccgg taatacgata gagttcggtt gatggcgtca
    25921 ctgcaatcag tggcgcaagc gtctggggaa tgtcgctggt acggcttttc tcggtaaacc
    25981 cccaggaagg ttcccgccct tgctcaattg cggcccgctg aaagctcgcc gacagctccg
    26041 ggctgtaatt aaatcgcggc tgagtcacga accacccttt acgatcctta cggaagattt
    26101 tggattctgc ctcgaggttc agcaacgcct ggcgcagcgt catacgttta atgcccagca
    26161 gctctcccag ctctctttct gaagggagtt ttccacccgg cgtagtgatg ccatctttta
    26221 accaccgatc cagtttctct tttgcatttt ctacggtgga ttgatttccc gtcattgttt
    26281 aatccttttg gtttaaacca gatgaggcat cttacgctta cgcaaaaaaa gaaagcaatt
    26341 aacggcgtat gactttgcag ataattgtga caaagctctc tattgacgta accacagact
    26401 tataagggag atactcgcat tgctgagtgc tcacgacagg caaaaaaaaa cctgccatcg
    26461 ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg cggttggcgc
    26521 gacgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc ggggtcactt
    26581 ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg cggataggcg
    26641 gaaccagaac aacggcttcg tcagtaccgg aagcacgcat gttggtcagt ttcttaccgg
    26701 tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc tgaccttcgt
    26761 aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg aacagcgcga
    26821 acgcgaccgc tttaccctga ccgttagaga tcagtacgcc gttctgacgc tgacccactt
    26881 cacccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca gtaccggaag
    26941 tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc acgtagtcga
    27001 gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta cgttcgccca
    27061 gcgcctgcat tacagaaccc tgatgctgtt cttcaacgtc cagagtcacg ttttcatatg
    27121 gctcttgttt acgaccgtcg atttcacgga agataacttt cggacgggat accgccagtt
    27181 cgaaaccttc acgacgcatg ttttcgatca gaacagacag gtgtagttcg ccacgaccag
    27241 aaacgcggaa cgcatcagcg tcttcggttt cttctacgcg cagcgcaacg ttgtgtacca
    27301 gttctttgtt cagacgatcc aggatctgac gagacgttac gaacttacct tctttaccgc
    27361 agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca acggagagtg
    27421 ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc agttcgccaa
    27481 ggcccgtgat cgcaacgata tcgccagctt ccgccaggtc ggtttcgata cgttccagac
    27541 cgaggtggcc cagcacttta ccgactttcg cgttgcgggt tttgccttcg ctatcgatga
    27601 tagtgacctg ctggttcggc ttcactttac cgcgcttgat gcggccaatg ccgataacgc
    27661 caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg tcaaggtcaa
    27721 cgtccggcgc aggaacgtgg tcaacaatcg cctggtacag cggggtcatg tcttccgcca
    27781 tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg atcgggaagt
    27841 ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga tccacaaccc
    27901 aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc ttcaggccgt
    27961 aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca aatgcgtcaa
    28021 ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca ccgaagtcgg
    28081 cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata gcggtgtttt
    28141 tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc acgcgctctt
    28201 gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct accagggtgg
    28261 ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg atcacaactt
    28321 tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact acaaaacagg
    28381 atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcga tttcacggag
    28441 cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt tccattgaag
    28501 cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg ttaagcatga
    28561 taacgccttt taggggcaat ttaaaagttg gcacagtttt cgctttatct tttttacggc
    28621 gacacggcca aaataattgc agatttcgtt accacgacga ccatgaccaa tccaggagag
    28681 ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag tgaagtttgt
    28741 tgatttgcgc ttcaccgata ctaaaggtaa agaacagcac gtcactatcc ctgctcatca
    28801 ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga ttggcggctg
    28861 gaaaggcatt aacgagtccg acatggtgct gatgccagac gcatccaccg cagtgattga
    28921 cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac ctggcaccct
    28981 gcaaggctat gaccgtgacc cgcgctccat tgcgaagcgc gccgaagatt acctgcgttc
    29041 cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc tgttcgatga
    29101 catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata tcgaaggcgc
    29161 atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg cagtgaaagg
    29221 cggttacttc ccggttccac cggtagactc ggctcaggat attcgttctg aaatgtgtct
    29281 ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag cgactgctgg
    29341 tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg aaattcagat
    29401 ctacaaatat gttgtgcaca acgtagcgca ccgcttcggt aaaaccgcga cctttatgcc
    29461 aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc tgtctaaaaa
    29521 cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg cgctgttcta
    29581 cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc cgaccaccaa
    29641 ctcttataag cgtctggtcc cgggctatga agcaccggta atgctggctt actctgcgcg
    29701 taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac gtcgtatcga
    29761 agtacgtttc ccggatccgg cagctaaccc gtacctgtgc tttgctgccc tgctgatggc
    29821 cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca aaaacctgta
    29881 tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc tggaagaagc
    29941 actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt tcactgacga
    30001 agcaattgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc gtatgactcc
    30061 gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt actacccggc
    30121 gcaacaacgc cgggatttag ttgccgtgga aactttcagc ccatctctgc atgggctttt
    30181 ttctccgtca attctctgat gcttcgcgct ttttatccgt aaaaagctat aatgcactaa
    30241 aatggtgcaa cctgttcagg agactgcttt atggcaacag gcacgcagcc cgatgctggg
    30301 cagatcctca actcgctgat taacagtatt ttgttaattg atgacaatct ggcgatccat
    30361 tacgccaacc ccgccgcgca acaactgctc gcccaaagct cccgcaaatt gtttggtaca
    30421 ccgttaccgg aactgttgag ctacttctca ttaaatatcg agctgatgca agaaagtctg
    30481 gaggcggggc aaggttttac cgataacgaa gtgacgctgg tcatcgacgg gcgctcgcat
    30541 atcctttctg tgacggccca gcgtatgccg gacggcatga tcctgctgga gatggctccg
    30601 atggataacc agcgccgctt aagtcaggaa cagctacagc acgcccagca ggttgctgcc
    30661 cgtgatttag tgcgcggcct ggcacatgag attaaaaatc cgcttggcgg tttacgtggc
    30721 gcggcgcagc tactcagcaa agcgttacct gacccatcac tactcgaata taccaaagtg
    30781 attatcgaac aggcggaccg gctgcgaaat ctggtcgacc gtctgttggg gccgcagctg
    30841 cccggtacgc gcgttaccga aagtattcac aaagtagctg aacgcgtggt aacgctggtg
    30901 tcgatggaac tgccggacaa cgtgcggttg attcgtgatt acgatcccag cctgccggaa
    30961 ctggcgcacg acccggatca aattgaacag gtcttgctga atattgtgcg caatgcgcta
    31021 caggcgctgg ggccggaggg tggtgaaatc attctgcgta cccgcaccgc gtttcaactg
    31081 accttacacg gcgagcgcta ccggctggcg gcgcggattg atgtggaaga taacgggccg
    31141 ggcattccgc ctcatttgca ggatacgctg ttttacccga tggtcagcgg ccgcgaaggt
    31201 ggcaccgggc ttggcttatc catcgctcgt aatttgattg atcagcattc aggcaaaatt
    31261 gaatttacca gttggccagg gcataccgag ttctcggttt acctgcctat caggaaataa
    31321 aggtgacgtt tatgcaacga gggatagtct gggtagtcga tgacgatagt tccatccgtt
    31381 gggtgcttga acgtgcgctc gctggggcag gtttaacctg tacgacgttt gagaacggcg
    31441 cagaagtgct ggaggcgctg gcgagcaaaa cgccggatgt gctgctttca gatatccgta
    31501 tgccgggaat ggacgggctg gcgctgctca agcagattaa acagcgccat ccgatgcttc
    31561 cggtcatcat tatgaccgca cattccgatc tggatgctgc cgtcagcgcc tatcaacaag
    31621 gggcgttcga ttatctgccc aaaccgtttg atatcgacga agccgtggcg ctggttgagc
    31681 gcgccatcag tcattaccag gaacagcagc agccgcgtaa tgttcagctt aacggcccaa
    31741 cgaccgatat catcggcgaa gcgccagcca tgcaggacgt gttccgtatt atcggtcggc
    31801 tttcgcgttc ttctattagc gtgctgatta acggcgaatc cggcaccggt aaagaactgg
    31861 tcgctcatgc cctgcatcgc cacagtccgc gcgccaaagc gccgtttatc gcgctgaata
    31921 tggcagctat cccaaaagat ttgatcgaat cagaactgtt tggccacgag aaaggcgcgt
    31981 ttactggcgc gaataccatt cgtcaggggc gttttgaaca ggccgatggc ggtacattat
    32041 tcctcgacga aattggtgat atgccgctgg atgtgcagac gcgtttgctg cgcgtgctgg
    32101 cagacggtca gttttaccgc gttggcggct atgcgccggt gaaagtggat gtgcggatta
    32161 tcgctgccac tcaccagaat ctcgaacagc gagtgcagga aggtaagttc cgtgaggatc
    32221 tgttccaccg cctgaacgtt atccgcgttc atctgccgcc gctgcgcgaa cgtcgggaag
    32281 atattccccg tctggcgcgc cattttttac aggttgccgc gcgcgaactg ggcgtagaag
    32341 cgaagttact gcatccggaa accgaagctg ctctgacgcg tctggcgtgg ccaggcaacg
    32401 tgcgccagct ggaaaacacc tgccgctggc taacggtgat ggccgccggg caggaagtgt
    32461 tgattcagga tttgcccggc gaactgtttg aatcaacggt tgcggagagt acttcgcaaa
    32521 tgcaaccgga cagctgggcg acgcttcttg cgcagtgggc agacagagcg ctgcgttccg
    32581 gtcatcaaaa tctgctttcc gaagcgcagc cagagctgga gcggacgtta ctgacgaccg
    32641 cgttgcgaca tacgcagggg cataaacagg aagcggcgcg gctactcggc tggggccgca
    32701 acaccctgac gcgtaagtta aaagagctgg ggatggagtg attcacagct tgtgtgtaaa
    32761 gattgattat tgagcgcaaa ttgctggtat tttacgcttt actgttccga taagttcagt
    32821 atgatcttgc ccggaaaacg gggagagtca ttatgctgga atcaataatt aatctggtat
    32881 cgagtggcgc agttgacagc cacacaccgc aaactgccgt tgctgccgtg ctgtgtgccg
    32941 caatgattgg gctgtttagt tgagtttcag attgctgatg aagtgctctt tgttcatgcc
    33001 ggatgcggcg tgaacgcctt atccggccta caaaagcaat attttcaaca ggttttgtag
    33061 gcctgataag cgtagcgcat caggcaatct tgttctcagc acattgatgg cgctttcgtt
    33121 ttctactttg taaacgaagc gccattcact atttaaatca cccgtgagaa ctgctgcatc
    33181 cgcgctttct ggcgcagata ggtatcaaag cacatgcaaa tgttgcggat cagcaagcga
    33241 cctttcgccg tcacctgtat tcccttctca tccacatcca ccagcccatc ttttgctaac
    33301 ggggcgagca gcttgagatc ttccgcaaag taatcagcga agtgcaaatc ccactgtttc
    33361 tcaatagggg cgtaatccag acggaagttg cagatgagcg acttaatcac atcgcggcga
    33421 atacagtcat cacgcgttag cgcaatacca cgccacagcg cattgccttg ttcatccact
    33481 tgctgatagt actgcttcaa ctctttctgg ttctgcgcgt agcagtcgcc aatcatgctg
    33541 atggcggaaa cgcccatccc cagcagatcg gtatcgccct gagtggtgta gccctggaag
    33601 ttacgatgca gcacgccttc acgctgggcc accgccagct cgtcatccgg acgggcaaag
    33661 tgatccatac cgataaactg atagcccgat tgcgtcagga aggcgatggt ttcctgcagg
    33721 atatcgagtt tttgctgcgg actcggcagg tcagcatctt tgattttgcg ctgagcagca
    33781 aaaatggtcg gcagatgcgc gtagttaaag acactcagac gatcggggtt cagctccgcc
    33841 acacgtttca gggtaaaggc gaaactctcc ggcgtctgtt tcggcaggcc gtaaatcagg
    33901 tcgatgttgg tggaggtaaa accaatctca cgcgcatggt taagcagtgc aaagatgaac
    33961 tcttcatcct gctcgcggtt aaccagacgt tgcacttctt tgttgaagtc ctgcacgccc
    34021 atgctcaggc gattaaagcc ttcggcgcgt aaatgatcga gtacatccag ttcgatttcc
    34081 cgcggatcga cttcgatcga aatctccgca tcggcattga actggaagtt ttcgcgcagc
    34141 agcttcatca ggcggctgat ttgcgcttta ttcagatacg tcggcgttcc gccgccccag
    34201 tgcaattggc tgacgtgacg cccggcgaac agcggtgcac gatggacgat ttcttgctcc
    34261 agcgcgtcca gatactgatc ggccttgtgc tgctggcgag taacaatctt attgcaaccg
    34321 cagaagtaac aaagtttatg gcagaacggg atatgtacgt agagagataa tggacgctca
    34381 ggatagcgcg ccacggcttg taaaaacgcc tgttcgccga agtcttctga aaactccagc
    34441 gcggtcgggt acgaggtgta tcgtggcccg gaatagttat atttctggat cagggccaga
    34501 tcccagtcga tttgctgtac agacatgctc actccttccg atggcgtctc tggcggctac
    34561 ggcgtaccgg cggatgcccg tttcgggtca tcagccggtt gcgcagccac ttttgtcgcc
    34621 gcgacaaccg ctgtagttta acgaataacc acaccagata acatataact ggaagggtta
    34681 tcagcagaac gggaaggccc acggcggcaa tccgttagtt gccccgcagc agacgcatca
    34741 tgtcttcttg cttctcgtct tcttcctctt cttcgtcatc gtcataagag aggccgagtt
    34801 tctgcatcaa ctcatcaata cgatccagtt tagcatccac ccaggattgc tcttcggcac
    34861 tcagggtttc gcctgcttcc agacgttcca gcagcgcatc cagacgctca tccgtttcca
    34921 gtaattccaa ctccgcctgc ggtgaaagca taggtttctc actcttcggt ttgtgctgtt
    34981 tggtgacttt ttcagtcacg cccaatggaa taggggtttt actgccaata cgtggatctt
    35041 ttggtgcgtt ctggcctttg ctgcctgacg tggtgtttcc gcccgctgcg cggctgcccg
    35101 gcgcatgacc acgacgtttt ttctgacgct tgcgatcacg agcttcctga tccagctcct
    35161 cgcgtgtttt tcgacgcgct tttgcatgac ctttgctgcg tgagtttgaa gatgatggtt
    35221 tcataatggt tcgtctttta gccgttttat tcagtataga tttgcggcgg aatctaacag
    35281 aaagcaagca aagaaaaaag gcgacagatt actctgtcgc cttttttcct gactcataac
    35341 ccttaacggg tcttacaatc cttgcaggcc gccaacacac cctgtttttc ctttagccgg
    35401 aacaatccgt gttgattcca tttccgttta attacgtctc cggacgtttg tcttcctgac
    35461 aatcctctgt cttcgcctcc tggcgctcct gaccctcatc ctgagtcatt gttcctgtta
    35521 gcgtcctcgc tctatgggtg ctactttacg cctttgcttt aaacaaacaa tcaaccgcca
    35581 tcagtatttg cacattttca gagttttata taaaagcaat tcactgattt atatagctta
    35641 tttgacatgc atcgcagaat tttctctgct atttcgcata aagactatcg gcaatgtctc
    35701 acaaagcgta tggcttttac ttacaacgct cacgccgaaa aatccgcctt ttattatggt
    35761 tcaggtataa tcgccacaaa gctttgactg acggagacga ccgctttgac taatttgaat
    35821 tatcaacaga cgcattttgt gatgagtgcg cctgatattc gccacctacc ttccgatacc
    35881 ggaattgaag tggcttttgc aggccgttcc aacgcaggta aatccagcgc gctgaacacg
    35941 ctgactaacc agaaaagcct ggctcgtacc tcaaaaaccc cagggcgcac ccagcttatc
    36001 aacctgtttg aagtggctga cggcaagcgt ctggttgact tgcctgggta cggttatgcg
    36061 gaagtcccgg aagagatgaa gcgcaaatgg cagcgtgcgc tcggcgaata cctcgaaaaa
    36121 cgtcagagcc tgcaaggtct ggtggtgcta atggatattc gccatccgct gaaagatttg
    36181 gatcagcaga tgattgagtg ggcggtagac agcaatatcg ccgttctggt gctgctgacc
    36241 aaagcggaca aactggcaag cggcgcacgt aaagcgcaat tgaatatggt gcgtgaagct
    36301 gtactggcgt ttaacggtga tgtgcaggtt gaaacgtttt cttcgttgaa gaaacaaggc
    36361 gtggacaagc tgcggcagaa actggatacc tggtttagcg agatgcagcc tgtagaagaa
    36421 acgcaggacg gcgaataatt ttcttgcctt aatgcttgtg ccggatgtgg cgtatccggc
    36481 ccgtaaattc aatagattat aattctaccg ttggcctgat aaacatagcg catcaggcat
    36541 tacggatctt ttctttcgcc caataaaaaa cgccccagtc attactgact ggggcggcta
    36601 aaatattcag ccaaatccga ttacgtgaag taaaaggtct gaaagataga acatcttacc
    36661 tctgtaccct acgcgactaa ctttactctt ttttgttcag ttcagaaagc actttttgta
    36721 attttttttc agttgttgca taggaatttc taatagccat cacaaaacgt gacagcttat
    36781 gttgcttact tacgaaaaaa gggcatgttc aggcgaatct tagtgcgcct gatcccagtt
    36841 ttcgccactc cccacttcca ccagcaacgg cacatccaga cgggtacagt tttccatcag
    36901 ttgatgaatc tgcttcgcga cggcatcaac atcatcttta tgaacttcaa ataccagttc
    36961 atcgtgtacc tgcatgatca tacgtacacg cggttgctca gcctgtaacc acgcatcaac
    37021 ggcaatcatc gcccgtttga taatgtcggc ggcggttccc tgcattggcg cgttaatggc
    37081 tgcacgttca gccgctgcac gacgagcacc attgctggat ttgatatccg gcagatacag
    37141 acggcgtccg tccagcgttt caacgtagcc ctgctctttc gcctgagcac gggtgcgttc
    37201 catatactcc agcacgccag ggtagcgttc gaagtaaagg tccatgtact tctgcgcttc
    37261 tttacgtgga atgttcaatt gccgcgccag accgaaagca ctcatgccat aaatcagacc
    37321 aaagttgatc gctttcgcgc tacggcgttg ctcgctggtg acggtttcca gtggcaaacc
    37381 aaacacttct gccgccgttg cccggtggat atcttttcct tccgcgaatg cggtcagcaa
    37441 gcctttgtca cgcgaaagat gcgccataat gcgcagttca atctgcgagt agtccgctga
    37501 gacaatcaca taatcctctg gcgcaataaa cgcctggcgg atacgacgac cttcttcgtt
    37561 acgcaccgga atgttttgca ggttaggatc ggttgacgat aaacgtcccg ttgcagttac
    37621 tgcctggtga taagaggtat gcacacgccc ggttttcggg ttgatcatca gcggcagctt
    37681 gtcggtgtag gtcgatttca gcttcgccag accacgatac tccagaatca cttttggcaa
    37741 cggatagtcc agcgccagtt cttccagtac ctcttccgac gttgacggcg cgccacccgg
    37801 cgttttcttc agcggtttaa tgccctgttt ttcaaagaga atggtttgta actgcttggt
    37861 ggaagaaagg ttaaattcct cacctgcaat ttcatgcgct ttcttttcca gctcagccag
    37921 acgaagggtg agctcttcag aatgattgtg cagcactttc ggatcgatct tcacaccgtt
    37981 acgttcaatg cgtgaaagca ccggcaccag cggcatttcg atattctcga agacgttcaa
    38041 cggccctttg tgtttttgca gatccggcca cattttcaga tgcaactgca aggtgacatc
    38101 tgcatcttcg gcggcgtaac gtccggcttc ttcgagggca atctggttaa aggtcagttg
    38161 atttttgcct ttaccagcaa tctcttcaaa agtgatggtt ttgtgcttca accaacgttc
    38221 cgcgaggctg tccatatcgt gacgcccggc aacgctattg agaatgtagg actccagcat
    38281 ggtatcaaac gcaatcccac gcagttcatt gccgtagttc gccagaatac cgcgatcgta
    38341 tttcaggttt tgcccgacct tcagcgcctt ttcatcttcc agcagcggtt ttagcaactc
    38401 gagtgcacgc tcgcgagaga tttgatcggg cgcatcaaga taatcatgag caaccggaat
    38461 atatgccgct acgcctggct cgatagcaaa agaaagcccg accaggttag cagagatgtt
    38521 atcaaggctg tcggtttcgg tatcaaatgc aaataccggc gctttttcca gcttcgcaat
    38581 ccacgctttc agtgtttctt catcaaggat ggtgacgtag ttgtcataag aaatcaccgt
    38641 tgccgtcact tctggtgctt cgtctgcaac actggtttcc tgtggcttcg cggctggttt
    38701 tgcccctttg gcctgtaacc atttgcccgc ttcgacatca gcagtccagc gtttgaactc
    38761 atactttttg aacagcccca acaactcttc cgctgccggt tgctgcactt ccagttgttc
    38821 acaggtcagc tccagttcaa cgtcggtttt aatcgtcgcc agctggtatg agagataagc
    38881 aacttctttg ttttgctcga gcttcgctgc cattgttttc gcgccacgga agctcaaccc
    38941 agcaattttt tctggctcgg catacagcgt atccagcccg cccagacctt gcagcaatgc
    39001 ctgcgcggtt ttttcaccaa cgcccggtac gccagggatg ttatcggagg agtcacccat
    39061 cagcgccagg aaatcgatga tcagttctgg cggcacgccg tacttattca ccacctcttc
    39121 cggtccgagg atggtattcg tcatggtatt gataagcgta atatttggcg tcaccagctg
    39181 cgccatatct ttatcgccag tgctgatcag caccggacgc ccggcttttt cggcttcgcg
    39241 cgccagagta ccgataacgt cgtccgcttc tacgccagaa accgccagca gcggcagtcc
    39301 catcgcttta accatcgcgt gcagaggttc gatttgtgct cgcagatcgt ccggcattgg
    39361 cgggcgatgt gatttgtaat gttcaaacag ttcatcacga aaggtttttc ccttggcgtc
    39421 aaagaccacc gctgcatgcg tcggtttata ttgcatgatc agactgcgca gcatgttgag
    39481 gacaccatac atcgcaccgg tcggctcgcc tgcgctgtta gtcagcgggg gaaacgcgtg
    39541 atatgcgcga taaagataag atgaaccatc tacaaggata agtggatttt gggggatctg
    39601 aaccataatg tccgtgcctg tttatcagat tatgggtaaa ggatgccaca gacaggatga
    39661 aaacatgagt ttttcaccgc ttttcgtgaa aatcttttgt agatcttctg gatcgctcgc
    39721 aaatcgtcat gtggataact ttgtgcataa attatttaca tcgcttttaa tttattatca
    39781 tgctttttac agcatgatgt ttgtctttgt ttttcatgca gttaaaaata aagccatccc
    39841 taataattga gataatggga ttttttctaa tgtggataga tatgaattat ttttctcctt
    39901 aaggatcatc cgttatttgg gtcgttttct gctaaaatct gcgccttgcg cttctttagc
    39961 gcactgtcta tggctaacct tttgaataaa ttcattatga cgagaatact cgctgcgata
    40021 acccttttgc tgagtatcgt attgaccatt ttggtcacta ttttttgttc cgtcccgatc
    40081 atcattgccg ggattgtaaa actattgcta cctgtgccag tcatttggcg aaaggtttcg
    40141 cgtttttgtg attttatgat gtattgctgg tgtgaaggtc tggcggtatt actgcatctg
    40201 aacccacacc tgcaatggga agttcacggg ctggaagggt taagtaagaa gaactggtat
    40261 ctgcttattt gtaaccaccg tagttgggca gatattgtcg tactgtgcgt gctgtttcgt
    40321 aagcacattc cgatgaataa atatttcctc aaccaacagc tggcctgggt gccatttctt
    40381 ggcctggcgt gctgggcgct ggatatgccg tttatgaagc gttattcccg cgcttattta
    40441 ttacgtcatc cggagcgacg tggtaaagat gttgaaacca ctcggcgttc ttgtgaaaag
    40501 tttcgcctgc atcccaccac cattgtcaat ttcgttgaag gctcccgttt cacgcaagaa
    40561 aaacatcagc aaacccactc cacttttcaa aacttgttgc caccaaaggc ggcaggcatt
    40621 gcgatggcgc taaatgtact gggtaaacaa ttcgataaac tgcttaatgt tacgctgtgt
    40681 tatccggaca ataaccgtca gccgttcttc gatatgttaa gcggtaaact gacgcgaatt
    40741 gtcgttcatg tggatttaca gcctattgcc gatgagttac atggcgacta catcaatgac
    40801 aaaagcttta agcgccattt tcagcaatgg ctgaactcac tgtggcagga aaaagaccga
    40861 cttctgacgt cgttgatgtc atcacagcgg caggacaagt aaattttgct cgcctctgaa
    40921 tgagcagagg cgagtgagta ttttaatgaa caaaacgtcc ggcacgagac ataaattctt
    40981 cttcgctcat ctcctgtccg ttaaaaacaa ctttacccgg tgtataacgc agttgcagcg
    41041 aggcggtatt gttgtcaatc gtgattaact gaaacatctg acctaaggtc atcatcccgc
    41101 tgatttgttt atcagcccgc ttttgcgctt tttccgcatc cattccttca gataaattaa
    41161 gctgttttgc ggtttctgtg acgacattaa gcggtaactt cacatcaaaa ttgagcgatt
    41221 taatatcttt gtttgtggat gatgaagatt tggctgggtc ggcaatactg atatccagat
    41281 tggcatttag ttcgccgagt gcgttcttcc attttaccgg ttgtttaatg gtcggctcac
    41341 ttttttgtaa taacggtaaa tactctttga acaatgcagc attcacttct tgcagagcaa
    41401 cttcatcgtt tgctaactca gggtgtgcag caagctgctt ctgcattgca atgttatact
    41461 ggatgataaa ttggcgcacc gctgagggat caatagattc agcaatcaat gaccactcac
    41521 cactaccgag ttgctgattc ccttttgtta acttatcaag ttcgtaggtc agattgacat
    41581 tgatataatc tttgtcttta acgcgatcca gcatggtttt tgcaacgaaa gcatcgattt
    41641 gggcaacgtc ttccccgtga ttgataatgt taatctgatt aaatttcgac tcgctttcac
    41701 caaccggaaa tttcttttct tctaatcgcg ccagagaatc aatggtaaaa ctttttgccg
    41761 ttactttgtt cattggtgat aattgcagaa caatatcttc gactttgcct tcaatatcca
    41821 ggttagacaa tgaactacca tcaccattaa aagtaaactg gccctcacca aaagaaaact
    41881 ttccatactc tgcaggggca acatctaatg tcgtagagaa tgagccacca aacgcgaatc
    41941 gcaatgtcgc ttccacgggc gatttatttt tcgcggcgat aaatagcggt tgtgtcagtt
    42001 cattattcac taagttcact tttgccgctg ccagtgctgg gatgacatta ccatgcatta
    42061 acattgtgat gggcaacgga ccgtgctcaa catccacatc aaatacaact ttctggcctg
    42121 gcttgatatt gagatcgggt gcaccgttat cgaatgtcat ttgcatttga aaacgagaat
    42181 taaaaaaacc tttgtcaaaa tttttataac ttaacgtcat atcgtaggca tgttcacctt
    42241 tctttttagc atcgttaaaa tctttaataa acttttcgac accaggctga atttgcgtac
    42301 ctgtgtacca tgtgccacca ccccagatta cggctaacgc aacaataaca cctgtagctg
    42361 acttacgtat catataaata ttccctgata aaacagcaaa actttaaata tccgcttatg
    42421 ctgaaataat gtccagcggc aggacgcatt atcagaatgt atttatattt attaaataat
    42481 aaaaaaaacc cgtgaatatt cacgggcctt atgtaattta cattgaatta ttttttctcg
    42541 gacagatatt tcactgtatc agcatactgc tgaacaaaaa catccatatt gctggtatcc
    42601 ataccctgcg gattcagctg atatttaccg ttaacaaaca tcgccggaac gccacgcaat
    42661 tgcacgtcag ctgcagcttt ttcctgctga gcgaccagag atttgaccac gaagctgttc
    42721 cacgccgcgt cgtactcttc acctttaata cctgcgttga taaatacatc gcggatatca
    42781 gatgctgaac gaatggtctg ggttttctgt acgccttcaa acagcggaac agtgactttg
    42841 tcttccacgc ccagcgccat cgccacagcc catgcctgag tcagatcttt gcccaggtca
    42901 ccacccatga agttgacgtg gtatttagtc atcttcacgc cttccggcag ttttttcttc
    42961 acattatcag aaatatgcag aacttcttca aactgatagc agtgcggaca gaagaaagag
    43021 aaaaactcca gcacttgcgg cgcgccagct accggttttt ccagggtagt gtactgttta
    43081 ccatcttcat actgcgccgc cgatgcgcta aacgctaaaa ctaaaccagc cagcgccagc
    43141 caaatctttt tcatgatcta ctctctccga ttaatacata ggtgttaatt gtaaaggggg
    43201 ttcttgtaga acttttgcct gttctataaa agtcgccgtt tgtcgcagcc agtaatcttc
    43261 cccggttaac cacgggaaat ttttcgggaa cgcgggatca gcccaacgcc gcattagcca
    43321 ggcaagataa taaaccaaac gcatggcgcg taaaggttca atcagtccga tttcagcggt
    43381 gtcgaactcg ctaaattctt cataagcttc aataatagtt tccagttgca tccgctgctc
    43441 ggctttatcg ccattgagca acatccacaa atcctgaata gctggaccat tacgtgcatc
    43501 atccagatca acaaacattg gaccatcgcg ccagagaata ttcccggcgt ggcagtctcc
    43561 atgtagccgc agaacggtga aatcttcccg ccagtgtgct gtaacggcgg cgatcagctc
    43621 atctgtcgct ttcaggaatg ccgctttcaa cccggaaggt atcagtgtag cgtcctcaaa
    43681 cagcttgcgt ggctcaatga gatattcatt cagaccgatg gtcgggcgat ggataaaaag
    43741 ctgtttgtgc cccgtctggt gcatacgccc taaataacgc ccaaccgctt ccgtctgatc
    43801 gatattatca gcttcgaact ggcgaccacc gacgcttgga aaaacagcga aataaaatcc
    43861 ctgatgattc aataaagtct gaccgttaaa ggccacaggt gctgcgaccg gaacttcatc
    43921 atttaccagc tgcaacgcaa attgatgttc ttcgaggatt tgatcggctg tccaacgttc
    43981 agggcgataa aatttgacga caaaacgtcg acgctcttcg tcctgaaatt gatagacacg
    44041 gttttcatag ctgttaagcg gggtaagacc ggaatccacc cggatccctt gctcaaacag
    44101 agcgtccatg atggtatccg ggtgtagtgt ctggaaagta aaagcgctgt tgttcatccc
    44161 atcatccgga aaacattacg aatgatgaag gatatcattt cacgatgatt ttggtggcgc
    44221 cgcttacaag cttttactct ttaattacgc cgcgagcgcg taacagcgcc gttttgaagt
    44281 cttcttcata atctttctgc aaacccggga tcaccgcatc tttcgcggaa tcacgcattt
    44341 tcaggtgata gatcagaatg tcatccgtca aatccgccag ttcgccgtca aaacctgact
    44401 ctttcgccag tttctgcaaa aattgcaaca ggttaaggtc tggctctttt tgccaagctg
    44461 gctggaggag ttcaataact tcattcagac gtttacattt catagtgatg ctccttactc
    44521 ttgagacaga cacgttagca gggtcaatcc cacaataaaa gaggcgatat cggtgaatct
    44581 gatgacgacg ataacaggcg ttgtgctggc aggcggtaaa gccagacgaa tgggcggcgt
    44641 agataaagga ttactggaat taaatggcaa accgctgtgg caacatgtcg ctgacgcgct
    44701 tatggcgcag ctctctcacg tcgtggttaa tgctaatcgt catcaggaaa tctatcaggc
    44761 aagcggtctg aaagtgattg aagattcact ggcggattac ccaggccctc tggcaggaat
    44821 gctttcagta atgcagcagg aagcaggcga gtggtttttg ttttgtccgt gcgatactcc
    44881 ttatattccc gaggatttag cagcccggct taatcatcag cgcaaagatg cgcctgtcgt
    44941 gtgggtccat gacggtgaac gcgatcaccc gactattgct ctggtaaacc gcgctattga
    45001 gcctttatta ctggaatatc tgcaagcagg agaacgccgg gtaatggcat ttatgcgtct
    45061 ggctggcggt catgcggttg atttcagcga tcataaagat acatttgtta acgtgaatac
    45121 gccagaggag cttgcccaat ggcaggaaaa acgatgatac cgttactcgc ctttgccgcg
    45181 tggagtggta ccggaaaaac tacgcttttg aaaaaactga tcccggcatt atgcgccaga
    45241 gggatccgtc cagggctgat taagcatacg caccatgata tggatgttga taagccaggc
    45301 aaagatagct atgagctgcg caaggctggc gcggcacaaa ccatcgttgc cagccagcaa
    45361 cgatgggcct tgatgacgga aacaccagac gaagaagagc tggatctaca gtttctcgca
    45421 agtcgaatgg atacctcaaa gctggatttg attctggtcg aagggtttaa gcatgaagag
    45481 atcgcaaaga ttgtgctgtt tcgcgatgga gccggacatc gacctgaaga attagtgata
    45541 gacaggcatg ttattgctgt agccagtgat gtgccgctta atcttgatgt cgcattactg
    45601 gatattaatg atgttgaggg gctggctgat tttgtggtgg agtggatgca aaagcagaac
    45661 ggataagcaa gagtaggctg gataagacgc gccagcgtcg catccggcat ttttctgcac
    45721 cagcgcaaaa aggcccagtc ttccgaccgg gccttc
//
LOCUS       DAZKHC020000028        44527 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZKHC020000028 DAZKHC020000000
VERSION     DAZKHC020000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 44527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.028"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            15..74
                     /gene="ibsE"
                     /locus_tag="ACHOSM_RS09300"
                     /old_locus_tag="pgaptmp_001860"
     CDS             15..74
                     /gene="ibsE"
                     /locus_tag="ACHOSM_RS09300"
                     /old_locus_tag="pgaptmp_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HGU4558401.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(194..1627)
                     /gene="hldE"
                     /locus_tag="ACHOSM_RS09305"
                     /old_locus_tag="pgaptmp_001861"
     CDS             complement(194..1627)
                     /gene="hldE"
                     /locus_tag="ACHOSM_RS09305"
                     /old_locus_tag="pgaptmp_001861"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HGU4558402.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIVSLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNT
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1675..4515)
                     /gene="glnE"
                     /locus_tag="ACHOSM_RS09310"
                     /old_locus_tag="pgaptmp_001862"
     CDS             complement(1675..4515)
                     /gene="glnE"
                     /locus_tag="ACHOSM_RS09310"
                     /old_locus_tag="pgaptmp_001862"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HGU4558403.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGSRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4538..5839)
                     /gene="ygiF"
                     /locus_tag="ACHOSM_RS09315"
                     /old_locus_tag="pgaptmp_001863"
     CDS             complement(4538..5839)
                     /gene="ygiF"
                     /locus_tag="ACHOSM_RS09315"
                     /old_locus_tag="pgaptmp_001863"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HGU4558404.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6081..6701
                     /gene="ygiM"
                     /locus_tag="ACHOSM_RS09320"
                     /old_locus_tag="pgaptmp_001864"
     CDS             6081..6701
                     /gene="ygiM"
                     /locus_tag="ACHOSM_RS09320"
                     /old_locus_tag="pgaptmp_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HGU4558405.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            6765..8003
                     /gene="cca"
                     /locus_tag="ACHOSM_RS09325"
                     /old_locus_tag="pgaptmp_001865"
     CDS             6765..8003
                     /gene="cca"
                     /locus_tag="ACHOSM_RS09325"
                     /old_locus_tag="pgaptmp_001865"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HGU4558406.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8166..8987)
                     /gene="bacA"
                     /locus_tag="ACHOSM_RS09330"
                     /old_locus_tag="pgaptmp_001866"
     CDS             complement(8166..8987)
                     /gene="bacA"
                     /locus_tag="ACHOSM_RS09330"
                     /old_locus_tag="pgaptmp_001866"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU4558407.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9077..9445)
                     /gene="folB"
                     /locus_tag="ACHOSM_RS09335"
                     /old_locus_tag="pgaptmp_001867"
     CDS             complement(9077..9445)
                     /gene="folB"
                     /locus_tag="ACHOSM_RS09335"
                     /old_locus_tag="pgaptmp_001867"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HGU4558408.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGASFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9550..10167
                     /gene="plsY"
                     /locus_tag="ACHOSM_RS09340"
                     /old_locus_tag="pgaptmp_001868"
     CDS             9550..10167
                     /gene="plsY"
                     /locus_tag="ACHOSM_RS09340"
                     /old_locus_tag="pgaptmp_001868"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGU4558409.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10180..11112)
                     /gene="ttdR"
                     /locus_tag="ACHOSM_RS09345"
                     /old_locus_tag="pgaptmp_001869"
                     /gene_synonym="dan"
     CDS             complement(10180..11112)
                     /gene="ttdR"
                     /locus_tag="ACHOSM_RS09345"
                     /old_locus_tag="pgaptmp_001869"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HGU4558410.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQVL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            11319..12230
                     /gene="ttdA"
                     /locus_tag="ACHOSM_RS09350"
                     /old_locus_tag="pgaptmp_001870"
     CDS             11319..12230
                     /gene="ttdA"
                     /locus_tag="ACHOSM_RS09350"
                     /old_locus_tag="pgaptmp_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HGU4558411.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            12227..12832
                     /gene="ttdB"
                     /locus_tag="ACHOSM_RS09355"
                     /old_locus_tag="pgaptmp_001871"
     CDS             12227..12832
                     /gene="ttdB"
                     /locus_tag="ACHOSM_RS09355"
                     /old_locus_tag="pgaptmp_001871"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HGU4558412.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRNPIVEEICEHVHYIK"
     gene            12880..14343
                     /gene="ttdT"
                     /locus_tag="ACHOSM_RS09360"
                     /old_locus_tag="pgaptmp_001872"
                     /pseudo
     CDS             12880..14343
                     /gene="ttdT"
                     /locus_tag="ACHOSM_RS09360"
                     /old_locus_tag="pgaptmp_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
     gene            complement(14386..15399)
                     /gene="tsaD"
                     /locus_tag="ACHOSM_RS09365"
                     /old_locus_tag="pgaptmp_001873"
     CDS             complement(14386..15399)
                     /gene="tsaD"
                     /locus_tag="ACHOSM_RS09365"
                     /old_locus_tag="pgaptmp_001873"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGU4558413.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGSFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15637..15852
                     /gene="rpsU"
                     /locus_tag="ACHOSM_RS09370"
                     /old_locus_tag="pgaptmp_001874"
     CDS             15637..15852
                     /gene="rpsU"
                     /locus_tag="ACHOSM_RS09370"
                     /old_locus_tag="pgaptmp_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGU4558414.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15963..17708
                     /gene="dnaG"
                     /locus_tag="ACHOSM_RS09375"
                     /old_locus_tag="pgaptmp_001875"
     CDS             15963..17708
                     /gene="dnaG"
                     /locus_tag="ACHOSM_RS09375"
                     /old_locus_tag="pgaptmp_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGU4558415.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINNAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAENGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17903..19744
                     /gene="rpoD"
                     /locus_tag="ACHOSM_RS09380"
                     /old_locus_tag="pgaptmp_001876"
     CDS             17903..19744
                     /gene="rpoD"
                     /locus_tag="ACHOSM_RS09380"
                     /old_locus_tag="pgaptmp_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGU4558416.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(19822..20328)
                     /gene="mug"
                     /locus_tag="ACHOSM_RS09385"
                     /old_locus_tag="pgaptmp_001877"
     CDS             complement(19822..20328)
                     /gene="mug"
                     /locus_tag="ACHOSM_RS09385"
                     /old_locus_tag="pgaptmp_001877"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HGU4558417.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKRAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            20453..20528
                     /locus_tag="ACHOSM_RS09390"
                     /old_locus_tag="pgaptmp_001878"
     tRNA            20453..20528
                     /locus_tag="ACHOSM_RS09390"
                     /old_locus_tag="pgaptmp_001878"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20486..20488,aa:Ile,seq:cat)
     gene            complement(20582..21346)
                     /gene="nfeF"
                     /locus_tag="ACHOSM_RS09395"
                     /old_locus_tag="pgaptmp_001879"
                     /gene_synonym="yqjH"
     CDS             complement(20582..21346)
                     /gene="nfeF"
                     /locus_tag="ACHOSM_RS09395"
                     /old_locus_tag="pgaptmp_001879"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HGU4558418.1"
                     /translation="MNNSLRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAADARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            21623..22246
                     /gene="nfeR"
                     /locus_tag="ACHOSM_RS09400"
                     /old_locus_tag="pgaptmp_001880"
                     /gene_synonym="yqjI"
     CDS             21623..22246
                     /gene="nfeR"
                     /locus_tag="ACHOSM_RS09400"
                     /old_locus_tag="pgaptmp_001880"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HGU4558419.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(22400..23920)
                     /gene="aer"
                     /locus_tag="ACHOSM_RS09405"
                     /old_locus_tag="pgaptmp_001881"
     CDS             complement(22400..23920)
                     /gene="aer"
                     /locus_tag="ACHOSM_RS09405"
                     /old_locus_tag="pgaptmp_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HGU4558420.1"
                     /translation="MSSHLYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            24338..25717
                     /gene="ygjG"
                     /locus_tag="ACHOSM_RS09410"
                     /old_locus_tag="pgaptmp_001882"
     CDS             24338..25717
                     /gene="ygjG"
                     /locus_tag="ACHOSM_RS09410"
                     /old_locus_tag="pgaptmp_001882"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HGU4558421.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(25759..26091)
                     /gene="ygjH"
                     /locus_tag="ACHOSM_RS09415"
                     /old_locus_tag="pgaptmp_001883"
     CDS             complement(25759..26091)
                     /gene="ygjH"
                     /locus_tag="ACHOSM_RS09415"
                     /old_locus_tag="pgaptmp_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HGU4558422.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            26310..27293
                     /gene="ebgR"
                     /locus_tag="ACHOSM_RS09420"
                     /old_locus_tag="pgaptmp_001884"
     CDS             26310..27293
                     /gene="ebgR"
                     /locus_tag="ACHOSM_RS09420"
                     /old_locus_tag="pgaptmp_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HGU4558423.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQEQEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            27477..30569
                     /gene="ebgA"
                     /locus_tag="ACHOSM_RS09425"
                     /old_locus_tag="pgaptmp_001885"
     CDS             27477..30569
                     /gene="ebgA"
                     /locus_tag="ACHOSM_RS09425"
                     /old_locus_tag="pgaptmp_001885"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HGU4558424.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            30566..31015
                     /gene="ebgC"
                     /locus_tag="ACHOSM_RS09430"
                     /old_locus_tag="pgaptmp_001886"
     CDS             30566..31015
                     /gene="ebgC"
                     /locus_tag="ACHOSM_RS09430"
                     /old_locus_tag="pgaptmp_001886"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HGU4558425.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            31078..32511
                     /gene="ygjI"
                     /locus_tag="ACHOSM_RS09435"
                     /old_locus_tag="pgaptmp_001887"
     CDS             31078..32511
                     /gene="ygjI"
                     /locus_tag="ACHOSM_RS09435"
                     /old_locus_tag="pgaptmp_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4558426.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            32645..33715
                     /gene="ygjJ"
                     /locus_tag="ACHOSM_RS09440"
                     /old_locus_tag="pgaptmp_001888"
     CDS             32645..33715
                     /gene="ygjJ"
                     /locus_tag="ACHOSM_RS09440"
                     /old_locus_tag="pgaptmp_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HGU4558427.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLIPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTNAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            33732..36083
                     /gene="ygjK"
                     /locus_tag="ACHOSM_RS09445"
                     /old_locus_tag="pgaptmp_001889"
     CDS             33732..36083
                     /gene="ygjK"
                     /locus_tag="ACHOSM_RS09445"
                     /old_locus_tag="pgaptmp_001889"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGU4558428.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            36409..38427
                     /gene="fadH"
                     /locus_tag="ACHOSM_RS09450"
                     /old_locus_tag="pgaptmp_001890"
     CDS             36409..38427
                     /gene="fadH"
                     /locus_tag="ACHOSM_RS09450"
                     /old_locus_tag="pgaptmp_001890"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HGU4558429.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPQVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     TAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(38472..38888)
                     /gene="higA"
                     /locus_tag="ACHOSM_RS09455"
                     /old_locus_tag="pgaptmp_001891"
     CDS             complement(38472..38888)
                     /gene="higA"
                     /locus_tag="ACHOSM_RS09455"
                     /old_locus_tag="pgaptmp_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HGU4558430.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(38885..39199)
                     /gene="higB"
                     /locus_tag="ACHOSM_RS09460"
                     /old_locus_tag="pgaptmp_001892"
     CDS             complement(38885..39199)
                     /gene="higB"
                     /locus_tag="ACHOSM_RS09460"
                     /old_locus_tag="pgaptmp_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HGU4558431.1"
                     /translation="MHLITQKALKDAAEKYPQHKTEFVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(39484..40620)
                     /gene="rlmG"
                     /locus_tag="ACHOSM_RS09465"
                     /old_locus_tag="pgaptmp_001893"
     CDS             complement(39484..40620)
                     /gene="rlmG"
                     /locus_tag="ACHOSM_RS09465"
                     /old_locus_tag="pgaptmp_001893"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HGU4558432.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            40705..41208
                     /gene="ygjP"
                     /locus_tag="ACHOSM_RS09470"
                     /old_locus_tag="pgaptmp_001894"
     CDS             40705..41208
                     /gene="ygjP"
                     /locus_tag="ACHOSM_RS09470"
                     /old_locus_tag="pgaptmp_001894"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HGU4558433.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            41285..41977
                     /gene="ygjQ"
                     /locus_tag="ACHOSM_RS09475"
                     /old_locus_tag="pgaptmp_001895"
     CDS             41285..41977
                     /gene="ygjQ"
                     /locus_tag="ACHOSM_RS09475"
                     /old_locus_tag="pgaptmp_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HGU4558434.1"
                     /translation="MLRAFARFLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            42056..43042
                     /gene="ygjR"
                     /locus_tag="ACHOSM_RS09480"
                     /old_locus_tag="pgaptmp_001896"
     CDS             42056..43042
                     /gene="ygjR"
                     /locus_tag="ACHOSM_RS09480"
                     /old_locus_tag="pgaptmp_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4558435.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            43325..44290
                     /gene="alx"
                     /locus_tag="ACHOSM_RS09485"
                     /old_locus_tag="pgaptmp_001897"
     CDS             43325..44290
                     /gene="alx"
                     /locus_tag="ACHOSM_RS09485"
                     /old_locus_tag="pgaptmp_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HGU4558436.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT        10589 a        12085 c        11515 g        10338 t
ORIGIN      
        1 agaaggaaag ggttatgatg aagttcgtca tcatactggt tgtgttgtta ctgttaagtt
       61 tcccgactta ctaacaactg atcagagggg ggagaaatcc tcccttaccc ttgttccttt
      121 actttaggtt gaaaaaacaa cagcgtcaat aggcctgcct gctacgaagc gagatctgtg
      181 aaccgctttc cggttagcct tttttatcct gttggatctt cttgatgatg ttagtggtcg
      241 agcaaccgtc ttcgaagttg agcaccagca cttcgccgcc gttggcccag acttctttac
      301 tcccggcgat ctcttctggt ttatagtcgc cgcccttcac cagtaaatct ggcaggatcc
      361 cggcgatcaa gcgctgcggc gtgtcttctt caaacgacac cacccagtcg accgcttcca
      421 gtgcgcccag cacaatcata cgctgttcga gcgtgttaac ggggcgggaa tcccctttca
      481 gccgtttggt ggaggcatcg ctgttcacag cgacaatcag acggtcaccc agtttgcgag
      541 catttgccag ataggagacg tgcccggcgt gcaggatgtc aaagacaccg ttggtcatta
      601 ccaccttttc gccgcgttta cgcgctgccg ctacggccag cttcagttcc tcttcggtca
      661 tcacgccaaa gccggtatct gcgcgcccac gcactgcatt ttccagctcg atcggcgaaa
      721 ccgtggaggt tcccagtttg ccgaccacca cgccagccgc cgcattggca aagaagcagg
      781 cttcttccag cgaattaccc gctgccagcg ttgccgccag gacgccaatc accgtgtcgc
      841 ccgcaccggt aacgtcatac acttcctgcg cctgggttgg catatgcagc ggcgctttac
      901 ccggttgcag cagcgacata ccctgttcgg aacgggtcac taacagagcc gagagttcgt
      961 aatcggcaat cagtttcatg ccgcgctcaa caatctcttc ttcggtctta catttaccga
     1021 caacagcttc aaattccgag agattcggcg ttaacagcgt agcgccgcgg tagcgctcaa
     1081 aatcggtacc ttttggatca atcagcaccg gaacgcccgc tttacgcgcc agttggatca
     1141 tctgctgtac gcttgccagc gcacctttgg cgtagtcaga aagcaccagc gcgccaatcg
     1201 aactcagcgc ctgattaatc cgctcgtgca gcggctgcgg atcaacgcct tcgaaacctt
     1261 cttcgaaatc cagacggatc agctgttggt tgcgggaaag tacccgtaat ttggtgatgg
     1321 tcggatgcgt cggtacagaa acgaagtcgc atttgacgtt gacgtcggcc agagatttac
     1381 tcagcgcgcg cgctgcatcg tcaatgcccg tcaacccgac caggcgtgca ttagcaccga
     1441 gagaaacgat attcatcgcc acgttagccg cgccgcccgg acgttcttcg atggtattca
     1501 ctttaaccac gggcaccggc gcttccggcg agatacggct ggtgggaccg taccagtaac
     1561 gatccagcat tacatcacca accaccatca ctcctgcacg ttcaaactct ggcagcgtta
     1621 ctttcattcc tgtctcctga gagattcaaa atttgcgcgc gataatacca tacttcattc
     1681 ttccaccagc cacttctgcc agcttgcccg caccagatca cgctctgcgg tgaagcaatc
     1741 ctccgacaca tggcccggca attcctgtaa tgccagatga tgaagttcat cgcgcagcgt
     1801 cgtgtaagca cgggtcagcg ccatcgcttc ctgctcttcc ataatgtcgt tttgcgccag
     1861 cagctccaga atacgcacgt tgtctgacca gcgcgttaac ttcggttttt catgagcgta
     1921 gcgcaacacc agatattggg taataaattc gatatcggta attccgcctt catcggcttt
     1981 gatatcaaag cgattacgat gtttattgcc gagatgagcg cgcattttct cgcgcatttc
     2041 ccgcacttcc gtttgcagag ttttaccttc acgcggcagc gtcataatct cgcgacgcac
     2101 tgcgtcaaag tgcgcggtga gctgcggatc gccgtacact acacgcgcac gcaccagcgc
     2161 ctgatgctcc cacgtccagg cctcgttttt ctgataatca gcaaatgctt ctgcggatgt
     2221 caccagcatt cccgccgccc cggacggacg cagtcgtgca tccacttcat acaaaatgcc
     2281 ggaagaggtt cgcgtactga acagatgcat aatgcgttgc gccagacgca gataaaactg
     2341 tcgcccgtcg atttcccgct cgccgtcagt catcgcatcc attgggcaat catggaggaa
     2401 gatcaggtca agatcggaac tgtagcctaa ctcccagccg cccagcttgc cgtagccgac
     2461 caccgcaaaa ccacggcctt cgcgatcgtt gaggtgattt ggcttaccgt agcgggccac
     2521 catttgaacc cacgcctgct gaacgacggc atctatcata gcctctgcca gccaggttaa
     2581 gtgatcgctc actttcatca ccggtagcgt accagcgata tccgctgcgg cgatgcgtaa
     2641 cagctgcgcc tgtttgaact gacgcagcgc ctcaagctgt tgctcttcgt catcttccgg
     2701 cacgcgcagc aaatactggc gcaactcatc gcggtaggca tcggtcgccg tcggctggta
     2761 aagggtgttt ggatcgagca attcatccag caataatgga taacgcgcca gttggctggc
     2821 aatcatcggc gacgcggcac acagagaaat caaatgttta agcgccgcgg ggaattcact
     2881 gagcaattct aaataggtgg tgcgggtaac aatccccacc agcaaggcgg taatgcgcga
     2941 cagcgtaacg gcagcgtctt cacgcgcaca gacatcactt agcagatgcg gcatcagatg
     3001 gtcgagcacc tgacgtcctc gcgacccgat ggtgcgctta tccagctctt tgcggaaatc
     3061 ggcaatcagc gttagcacct gtttgcgatc atcctcgcta agatgcgcca gcactggcgt
     3121 agtgtcatct tcctgcaacg catcctgcca cagctcacgc cactgttccg acagcgactc
     3181 ttcctgagtt tcactttcat cgtcgccaat caattcatta aacacccggc gcacattggt
     3241 catatgtgcg gtcagcgccc cggtcagttg cggccagtca gcaaagtcca tcgcccacgc
     3301 cagccgcgca cggttaagct catcagaagg aagcgtctgg gtttgttcgt cgttaatgct
     3361 ttgcagcagg ttttccagac gccgcaggaa cagatacgcc actcgcaatt gttcagcatc
     3421 gttttcagaa agcagatgca gcgcggcaat gacgctgagc gttggcagta aagagcgcga
     3481 ctgcagcgac ggttcgcgcc caccgcgaat gagctggaac acctgaacga taaattcaat
     3541 ttcgcgaatg ccgcctgcgc cgagtttgat attgtcggtc aaaccacgtc gacgcacttc
     3601 acgggcaatc atccctttca tgttgcgcag cgactgaatc acgctgaaat cgatgtaacg
     3661 acggaaaaca aacgggcgca gcatcgcacg caactcgtta gcatagacgc cttcgctatc
     3721 gcccataatc cgcgccttga ccatcgcgta acgctcccag tcgcgcccct gctcctggta
     3781 ataatcctcc aacgcggcaa agctcagcac cagcgggcca ctttcgccaa acggacgcag
     3841 ccgcatatcc acgcgataga cgaagccatc ctgcgttggt tgatccaaca ctttaatcag
     3901 ccgctgcccc atgcgggtaa aaaactgcgc gttatccagt tcccggcgtc cgccctgcgt
     3961 acaaccatgt tccggccagg caaaaatcag atcgatatct gaggagaaat tcagttcccc
     4021 accgcccagc ttacccatgc ctaaaatcag cagcggttgc gcttcgccct gcgcattgca
     4081 cggcgttccc cactcgcggc agcaagcgtc atacagccag tcacgcgcgg cgacaatcag
     4141 cgtctccgcc agatggctga gctgctgcaa tatgctctct tcagtaacca aagccagcgt
     4201 ttgcgcccag gcgatgcgca ccataatgcg ccgccggaat agccgcagct cgcgcattaa
     4261 cccggcttcg tcactcacat tgctgagcgc ctcctgcaac catgccgcgt aatgctgcca
     4321 ttcatcagcc tgcggcgatt ggctttccag ttccgtcagc cactctggat gcgcaatcac
     4381 gctgtcctgc acaaaatcac tgaaagtaag tactgacttc gcctgtgcgc taagtgattt
     4441 ctcggctaaa ggctctggca gccgctcaac aacggtctgc cagtactgct gtaacggtga
     4501 agagagcggc ttcataagcg attttatcct tgcctgatta acgttttccg ctgtgcaacc
     4561 agaacggttc ctgattgttt gcctcattac ggaaatgttc aatttcaatg cgttgcccgg
     4621 tcgcaatagc gtgacgcagc ccctgccagt tctccagcca ggcttgcgcg acgacaggat
     4681 catagtaacc cgccagcaac agtattgagt caatatcacg cgtcagacgt ggcaactggt
     4741 cacggtagcg atcgcccaac ggctggcaga aaacgctttt cagttcagcg gcatggcggg
     4801 aaagatggat atcggcaaag cgtttgaagg aatcgctcat tttactctgc gctttggcat
     4861 ctaaaaacgg ctgccaggct ttgcttacca gccattcggt caacgccagc ttcgccattg
     4921 ccgttttggt agagtagacc gccgtcacgg cagaaaccgc agaagcgatg gtcgcttcgc
     4981 attgagtcag caaatcacgt aagtgagtgc tcgctttgcg cggcacaata ccaccgaaca
     5041 gcatcagtgt atgacggacc agaccaatgg ctgccagcac ctgttctttc gccgcatcgt
     5101 tgccgcgtac ccacagttct tcatgatact gccattgcgc taacgccagc tcgagcgccg
     5161 cttccagccc ctgttccaca tcggcttttg ccgcaacatg caaaatggtg gtcggtttga
     5221 tttcacgcgc cggattgccc tgcgccagat gatagccacg cgccgctttg ctcaggctgc
     5281 cctggcgtaa tccggtttgc gataccagtt ggttcgccag tttcagcacc gcgcgcgtgt
     5341 cgccgctaag cagttccagt tccagctcac agataggttc agcaaattcc cccgctttca
     5401 cctccccctg atcgagagcg atttcaattc ggctgtcatc gaccgccacc agccattttt
     5461 cgcgataaaa atcggtgctg aacagcggtt gtacgcggga agcgagatcg gcgggcaatt
     5521 cgccgttcgg ccagacttcc gtcggtaact gcgccaggtc gagcgtcggt tcgctcaacg
     5581 ccacgttata ttccgggcgc tggtgtaagc cgcctgtcac tcttcctgca actttcatgg
     5641 tcatctcata gcgaccgttt tcgccacgaa tacgtaagcc catatcgtgc ccacgcagcc
     5701 agttatccgg cgtttcgtag taaatattca gcaactgcac ggggtcatgg tgctcgccgc
     5761 ccagcgtatt gagatggtca cgcaacgcct caacggcact gtgattaaca ataaacttta
     5821 attcgatttc ctgagccatg gcctttactt atcggttatg tcacataagt gacgatgaac
     5881 ggcgaactta atgcgatctt ttttgtcagt agatagtatt ttgcgccaaa ttgccatgca
     5941 acgggcaatt tgacgggcgt aaaagtttga agcagtggca agacctgaca cagaggattc
     6001 cgattgatgt cgaatgcttt gcgtagcgac actgatgcca ctactatcgt tccactttcc
     6061 atgacaataa cgacagcctg atgccaaaat tacgcctgat cggattaact ttactcgcac
     6121 ttagcgccac tgccgtctca cacgctgaag aaacgcgcta tgtttctgat gaactgaata
     6181 cctgggtccg tagcggtccg ggagatcatt atcgcctcgt gggcacggtt aacgccggcg
     6241 aggaagtgac cttattacaa actgacgcca acaccaatta tgcccaggtg aaagacagct
     6301 ctggccgtac cgcctggatc ccgttgaaac aacttagcac tgagccaagc ctgcgctccc
     6361 gtgtgccaga tctggaaaat caggtcaaaa ccctgaccga taaactcacc aatatcgata
     6421 acacctggaa tcagcgcacc gcagaaatgc agcaaaaagt ggcgcagagc gacagcgtga
     6481 tcaacggatt aaaagaagaa aatcagaaat tgaaaaacga gctgattgtc gcgcagaaaa
     6541 aggtcgatgc cgccagcgta cagttggatg acaaacagcg caccatcatc atgcaatggt
     6601 ttatgtatgg cggtggcgtg ctggggcttg gcttgctgct cggtctggta ctgccacacc
     6661 tgatcccaac gcgcaaacgc aaagatcgct ggatgaacta aatcgccttc ttcgttgcac
     6721 tgtcttatta ttaggaattg tgtatttgac gaagggatga tggcgtgaag atttatctgg
     6781 tcggtggtgc tgttcgggat gcattgttag ggctaccggt caaagacaga gattgggtgg
     6841 tggtcggcag tacgccacag gagatgctcg acgcgggcta ccagcaggta ggccgcgatt
     6901 ttcctgtgtt tctgcatccg caaacgcatg aagagtatgc gctggcacgt accgaacgga
     6961 aatccggttc cggttacacc ggttttactt gctatgccgc accggatgtc acgctggaag
     7021 atgatcttaa gcgtcgcgat ctgaccatta atgcgctggc ccaggacgat aacggcgaga
     7081 ttatcgaccc gtacaacggt ctgggcgatc tgcaaaatcg tctgttgcgc catgtttccc
     7141 ccgcttttgg cgaagatccg ttacgcgttc tgcgcgtggc gcgttttgct gcgcgttatg
     7201 cccacctcgg ttttcgtatt gccgatgaaa ctctgacgct gatgcgcgag atgacccatg
     7261 cgggtgaact ggaacacctg acgcctgaac gggtatggaa agagacggaa agcgccctca
     7321 ccacccgcaa tccccaggtg ttcttccagg tactgcgcga ttgcggtgca cttcgcgttt
     7381 tattcccgga aattgacgcc ctgtttggcg ttccggcccc tgccaggtgg catccggaaa
     7441 tcgacacggg tattcatacc ttaatgacgc tctcaatggc ggcgatgctg agtccgcagg
     7501 ttgatgtccg tttcgcaact ttatgccacg atctcggtaa aggactgacg ccgccagaac
     7561 tttggccgcg tcatcatggg catggcccgg cgggtgttaa gttagtggaa caactatgcc
     7621 agcgtctgcg cgtgccaaat gaaattcgcg atttagccag actggtggct gagtttcacg
     7681 atctcatcca caccttccca atgctgaacc caaaaaccat cgtcaaatta tttgattcca
     7741 tcgatgcctg gcgtaaaccg cagcgtgtcg agcaactggc gctgaccagc gaggctgacg
     7801 tacgcggcag aaccggtttt gaatcagcgg actacccgca aggccgttgg ttacgcgaag
     7861 cctgggaagt ggcgcagtca gtgccgacaa aagcggtcgt tgaagcggga tttaaaggtg
     7921 tggagattcg cgaggagctg acccgacggc ggattgcggc ggtagccagt tggaaggaac
     7981 aacgttgccc aaagcctgac tgagtataaa actaaacgtt aacacgaacc atcccctcgc
     8041 cccttcgggg agagggttag ggtgagggga acagaccagc accggtgcga acaatcaccc
     8101 ccaccccggc cctctcccta cagagcgagg gagaaaacca ggcctgctcc cgaacctttt
     8161 ctcaatcaaa agaacacgac gtacaccgca gccgccacaa taaagcgata aatggcgaac
     8221 gggataaacg aaatgcgctt aatcaattgc aggaaggttt taatcgctat cagcgccacc
     8281 acaaaagcgg tgataaaccc aacggcaaac atcgggatat cgccggttgt caggaagccc
     8341 cagcttttgt agagatcgag cgccgttgcg cccatcatca tcggtaccgc cagcaggaac
     8401 gagaactcgg aagcagcgta acggctcacg cccatcagca tcccacctga aatggtggcc
     8461 ccggaacggg agaaacccgg ccacagcgcc agacactgga aacagccaat cataaacgcc
     8521 tgacgatagg tcatatcatc aagacccggc gcacgcggct ctttcggctt caggcattcg
     8581 gcggcaatca gcaacaaacc gccaacgacc agtgcataca tcacatttat cgggttaaac
     8641 aatgacttaa tcgtgtcgtg gaacaacagc cccagtacca ccgccggaat catccccagc
     8701 aaaatgtgga tcagcgttaa acgacctttg ctttcacctt cgtgctgcaa cgggcggcca
     8761 aagtggatgc caatcaggcc aaacagacgc cgccagaaca tcactactac cgccagaatt
     8821 gatcctaact ggatcacaac ttcaaaggtt ttcgccgtgt cgccctcaaa ccccaacaag
     8881 tgaccgacaa taatcatatg gcccgtgctg gataccggca gaaattctgt caatccttcg
     8941 accacaccca atattgccgc tatcagcagc gagtgcatat cgctcatcaa taaaccccta
     9001 aattattaaa atgtaccgct tgtccgaact actgcgtatg accaggttat aaccgtttgg
     9061 tttaacagct gtaaaattaa ttattttctt tcagattatt gccacgctca atgattacgc
     9121 caacattcgc cgcccgcgcc actgcgcctg gcttgctgag tttgatacgc acccacggcg
     9181 agttgaagcg tgctaacagc agctccgcca cctcttcagc cacgcgttcc accagcgcaa
     9241 aactcgcccc ctcgacgtgg ctgaccaccg tttctgcaat gtcagcgtaa ctgaggcaat
     9301 ccgccacatc atcacttttc gccgctttac ggttatccca tgccatttcg atatcgaaca
     9361 ctaacttctg ttcgatggtc tgttcccagt cgtaaacacc aatagtggtg attaccgaaa
     9421 gttgctctat aaatacaata tccatcacgt cctgcctgct ttttggctaa ccggatacca
     9481 cttccggcga aatgtgcgta ttatccacag attcatcgtt gaacacgaat tttcaaaacg
     9541 gaacagctta tgagtgcaat cgcgcctgga atgatcctca tcgcgtacct ctgcggctcc
     9601 atttccagtg ccattctggt ttgccgcttg tgtgggctgc ccgatccgcg aaccagcggc
     9661 tccggcaatc caggcgcaac caatgtgtta cgtatcggtg gcaagggagc agccgtagca
     9721 gtactgattt tcgacgttct gaaaggaatg ttgcccgtct ggggcgcgta tgaattaggt
     9781 gtcagcccct tctggctagg cttaattgcc atcgccgcct gtcttggaca catctggccc
     9841 gttttcttcg gatttaaagg aggaaaaggc gttgctaccg cttttggtgc catcgcaccc
     9901 attggctggg atctcaccgg agtaatggcg ggaacctggt tactgaccgt gctattgagc
     9961 ggatactcgt cgctgggagc gattgtcagt gcactgattg ctccgtttta tgtctggtgg
    10021 tttaagccac aattcacctt cccggtttcg atgctctctt gcctgatcct gctgcgtcat
    10081 catgacaaca tccagcgtct gtggcgtcgt caggagacaa aaatctggac gaaattcaaa
    10141 agaaagcgcg aaaaggatcc cgagtgattt ctggtggatc tacatgacct gatagccttc
    10201 atcgggcttg cccagccgtt gctggcacca tgccgccaga aattccacgc agacacgtaa
    10261 tttcatgctg cgatagagcg gctcccggta aacagcccag atattggcgc tttgtgcata
    10321 ctccggcaat acttgcacca gtttgccact ctccagaaac ggcaacacat cccactcgga
    10381 acgcagcata atccctttgc cctccagcgc ccattgcaac actatctcgc cgctattgga
    10441 ggaaagatgc ccgctaacct tcaccgattt tttctcctga ccgttcccca actcccatat
    10501 tccgtgggtc atatcgcgct ctttggtcac cagacagtca tgacgactta attcctgtaa
    10561 tgattgtggc tgcggatatt tttgcagata ctcaggtgct gcgcataata ttcttttatt
    10621 tttcgttaac agatgcgcaa tataataatc aggaatttcg tcattaatac gaatatccag
    10681 atcaatatta tcctgcacta aatcaatttg ccgatcgaac aattcaaaat gcacctgtaa
    10741 ctcaggataa ttgcgcataa gttcggtaat ggctggcgca atatggctgc gcccaaaacc
    10801 aaaactgcaa ccaatacgaa tcatcccctc cgggcgcgtt ttgatttgcg tgacgtcatc
    10861 gaccagtcgc tgatattggg tgaggatttc cagcgcatgt tcgtaacaac gttggccact
    10921 ttcagtcagc gccacgccgc gagcggagcg gttcagcagc gttgttgcca gcgtattttc
    10981 aaggacttgg atccgcttgg tgacaaatgc aggcgtttgc ccaagcgtcg ccgctgcagc
    11041 actaaaactc ccgctgtgga cgatttctac cagtacctgc aagtctttgg ctaaaggcca
    11101 gctattcagc atcgttaatt atccgcagtt gtgataagcg cagtgtattt cgcaaaacat
    11161 tgccattccc tgctctggcg caatattcat tcttattttc gcgttgattc acgaactgtt
    11221 aattacatat tcacggtggc aaaaaatata aaaccacatt tttagtggta gtttgtggcg
    11281 gtgaattttt ccagacaaat acaaaaactg gagttgccat gatgagcgaa agtaataagc
    11341 aacaggcagt gaataagttg acagagattg tcgctaactt taccgccatg atttctaccc
    11401 gaatgcctga tgacgtggtg gataaactaa aacagctaaa ggatgccgaa acgtcgtcga
    11461 tggggaaaat tatctaccat acgatgttcg acaacatgca aaaagcgatt gacctgaatc
    11521 gtcctgcctg tcaggacacc ggggagatta tgttcttcgt taaagtcggt tcccgcttcc
    11581 cactgcttgg cgagctgcaa agcatactca aacaagccgt ggaagaggca accgtcaaag
    11641 cgccactacg tcacaatgcg gtagaaattt ttgacgaagt aaacaccggc aaaaataccg
    11701 gtagcggcgt accgtgggtc acctgggaca tcatccccga caatgacgat gcggaaatcg
    11761 aagtttacat ggcaggcggc ggctgcacgc tacctggccg ctcgaaagtg ttaatgccgt
    11821 cagaaggcta cgaaggcgtg gtgaaattcg tcttcgaaaa tatctccacc ctcgccgtaa
    11881 acgcctgtcc accggtactg gtgggcgtgg gcatcgccac ctcggtggaa accgccgccg
    11941 tactctcgcg taaagccatt ttgcgcccga ttggcagccg ccatcccaat ccaaaagcgg
    12001 cagaactgga gctacgcctg gaagaaggac tcaaccgtct ggggattggt ccacaagggc
    12061 tgaccggcaa cagttcagtg atgggcgtac atatcgaatc tgccgcccgc catccgtcaa
    12121 ccatcggcgt tgctgtctct accggctgct gggcgcatcg tcgcggcacg ctgctggttc
    12181 atgccgatct cacctttgaa aatctgtctc acacccggag cgcgttatga aaaagatcct
    12241 gacaaccccg atcaaagctg aagatctgca agatattcgc gtcggcgatg tgatctacct
    12301 gaccggtacg ctggtgacct gccgcgacgt ttgtcaccgc cgtttgatcg aactgaaacg
    12361 tccgatccct tacgatctca acggcaaagc gattttccac gctggcccca tcgtgcgcaa
    12421 aaacggcgac aaatgggaga tggtctccgt cggcccgaca accagtatgc gtatggaaag
    12481 ttttgaacgt gaatttattg agcagaccgg cgtgaaactg gtggttggca aaggtggtat
    12541 ggggccgctg accgaagaag gctgccagaa attcaaggcg ctacatgtga ttttcccggc
    12601 aggctgcgcg gtgctggcgg caacccaggt agaagagatt gaagaagtgc actggacaga
    12661 actcgggatg ccggagtcac tgtgggtctg ccgggtcaaa gagttcggcc cgctgattgt
    12721 ctctattgat acccacggca acaacctgat agccgaaaac aaaaagctgt tcgccgaacg
    12781 ccgcaatccc atcgtggaag agatctgcga gcacgtccat tacatcaaat aaccctcccg
    12841 gagaggctca cccctctcct ttttcgcagg cataacacga tgaaaccttc cactgaatgg
    12901 tggcgatatc ttgcgccgct ggcggtcatc gccattattg ctctacttcc ggttcccgca
    12961 gggctggaga accatacctg gctctacttt gccgttttta ctggcgtgat cgttggactg
    13021 atcctcgaac ccgtgccggg tgccgtggtg gcgatggtgg gtatctccat catcgccata
    13081 ctctctccct ggctgctgtt cagccccgaa cagctcgccc agccaggctt taagttcacc
    13141 gccaaatccc tctcctgggc agtttctggt ttttctaatt cggttatctg gctcattttc
    13201 gccgccttta tgtttggcac aggctatgaa aaaaccgggc ttggacgccg cattgcgctg
    13261 attctggtga aaaagatggg gcatcgcacg ctgtttctcg gctatgcggt gatgttctcc
    13321 gagctgatcc tggcacctgt aacaccgtcc aactcggcgc gtggtgcggg tattatctat
    13381 cccatcatcc gtaacctgcc accgctctat caatcacaac caaacgacag cagttcgcgc
    13441 agcattggct cgtacatcat gtggatgggg attgttgccg actgcgtgac cagcgccatt
    13501 ttcctgacgg cgatggcacc taacttgctg ttaattggac tgatgaaaag cgcatctcac
    13561 gccacgctga gttggggcga ctggttcctc gggatgttgc cgctcagcat tttactggtt
    13621 ctgctggttc cctggctggc ttacgtgctg tacccgccgg tactgaagtc tggtgatcag
    13681 gtgccgcgct gggcagagac ggaactgcag gcaatgggcc cgctctgttc gcgtgaaaaa
    13741 cggatgctgg ggctgatggt aggcgcgctg gtgctgtgaa ttttcggcgg tgattatatc
    13801 gatgccgcga tggtcggtta cagcgtggtg gcgctgatgc tgcttctgcg cattatcagt
    13861 tgggacgaca ttgtcagtaa taaagccgcg tggaacgttt tcttctggct ggcctcgctt
    13921 atcaccctcg ccaccggact caacaacacc ggttttatta gctggtttgg caaactgtta
    13981 gcaggcagct taagtggtta ttcgccgacg atggtgatgg tgacgttgat tgtggtgttt
    14041 tatctactgc gctacttttt cgccagcgcc acggcgtata cctccgccct cgcaccgatg
    14101 atgattgccg ctgcgctggc gatgccggaa atcccgctgc cggtgttctg cctgatggtt
    14161 ggcgcggcaa ttggtctggg gagcattctt acaccatacg ccaccggacc cagtccgatt
    14221 tactacggta gtggttatct gccaacggtg gattactggc gactgggggc gatttttgga
    14281 ctgatattcc tcgtgttgct ggtgattacc ggcttgctgt ggatgcccgt ggtgttgctt
    14341 taagtgagga cggtgccgga aattgctctc cggcaccgga tagttttacg cagccggtaa
    14401 ctccgccagt ggccagcgcg gacgcacgct aacgccgaga tccgccgtcg cgcctgcttt
    14461 aaaccgcacc attccggcat aggcgatcat cgcaccgtta tcagtacaaa attccggacg
    14521 cgcgtagaac acttcgccgc ggcgtttttt catcatttcc gccagcttcg cccgtaacgt
    14581 gcggttagca ctcacgccgc ctgccatgac cagtcgctta aagcccgtct gatccagcgc
    14641 acgcttacac ttaatcatca gcgtatcgac caccgcatct tcaaaggcgc gggcgatatc
    14701 agcacgcgtc tggtcgtcgg tgccgttgtc acgaatggta tttgccgcga aggttttcaa
    14761 accagagaag ctgaaatcca accccggacg gtcggtcatc ggacgtggaa agacaaagct
    14821 cccggcagta ccctgagccg ccattttcga cagtaacggt ccgccaggat aatccagccc
    14881 cagcagcttc gcggttttat caaacgcttc accggcggca tcatcaatag actcgccgag
    14941 cagctcgtac tgaccaatgc cagtcacgct gattaactgc gtatgaccgc cggacaccag
    15001 cagcgcgaca aacggaaatt ccggcgggtt atcttccagc atcggcgcta acagatgccc
    15061 ttccatatgg tgtaccggga ttgccggaac gttccaggca aacgccagag aacgccccac
    15121 ggttgcgcca accagcagtg cgccgactaa tccagggcct gcggtatagg ccacagcatc
    15181 aatgtctttt gccgttaaac cagactcctt tagcgccgcc tggatcaacg gtacggtttt
    15241 gcgcacgtga tcgcgggagg ccagttcagg cacgacgccg ccgtagtcag cgtgcaattt
    15301 cacctgacta tacaattggt tggctaacaa acctttttca tcgtcgtaaa tggcgatgcc
    15361 ggtttcatcg caggaagttt caatacccag tacacgcatg acttgtttta cctcgcttta
    15421 ttaccgcgca gtgtaggacc aatgcgggtt gatgtaaaac tttgttcgcc cctggagaaa
    15481 gcctcgtgta tactcctcac ccttataaaa gtccctttca aaaaaggccg cggtgcttta
    15541 caaagcagca gcaattgcag taaaattccg caccattttg aaataagctg gcgttgatgc
    15601 cagcggcaaa ccgaattaat caaaggtgag aggcacatgc cggtaattaa agtacgtgaa
    15661 aacgagccgt tcgacgtagc tctgcgtcgc ttcaagcgtt cctgcgaaaa agcaggtgtt
    15721 ctggcggaag ttcgtcgtcg tgagttctat gaaaaaccga ctaccgaacg taagcgcgct
    15781 aaagcttctg cagtgaaacg tcacgcgaag aaactggctc gcgaaaacgc acgccgcact
    15841 cgtctgtact aattccccga gagcgttgct ctccgatcag accgagttgt agttgtaagg
    15901 ccgtgcttcc gaaaggaatg cgcggcttat tttcgtttat gaattgctaa aaatcggggc
    15961 ctatggctgg acgaatccca cgcgtattca ttaatgatct gctggcacgc actgacatcg
    16021 tcgatctgat cgatgcccgt gtgaagctga aaaagcaggg caagaatttc cacgcgtgtt
    16081 gtccattcca caacgagaaa accccgtcat tcaccgttaa cggtgagaaa cagttttacc
    16141 actgctttgg atgtggcgcg cacggcaacg cgatcgactt cctgatgaac tacgacaagc
    16201 tcgagttcgt tgaaacggtc gaagagctgg cagcaatgca caatcttgaa gtgccatttg
    16261 aagcaggcag cggccccagc cagatcgagc gccatcagag gcaaacgctt tatcagttga
    16321 tggacggtct gaatacgttt taccaacaat ctttacaaca acctgttgcc acgtctgcgc
    16381 gccagtatct ggaaaaacgc ggattaagcc acgaggttat cgctcgcttt gcgattggtt
    16441 ttgcgccccc cggctgggac aacgtcctga agcggtttgg cggcaatcca gaaaatcgcc
    16501 agtcattgat tgatgcgggg atgttggtca ctaacgatca gggacgcagt tacgatcgtt
    16561 tccgcgagcg ggtgatgttc cccattcgcg ataaacgcgg tcgggtgatt ggttttggcg
    16621 ggcgcgtgct gggcaacgat acccccaaat acctgaactc gccggaaaca gacattttcc
    16681 ataaaggccg ccagctttac ggtctttatg aagcgcagca ggataacgct gaacccaatc
    16741 gtctgcttgt ggtcgaaggc tatatggacg tggtggcgct ggcgcaatac ggcattaata
    16801 acgccgttgc gtcgttaggt acgtcaacca ccgccgatca catacaactg ttgttccgcg
    16861 cgaccaacaa tgtcatttgc tgttatgacg gcgaccgtgc aggccgcgat gccgcatggc
    16921 gagcgctgga aacggcgctg ccttacatga cagacggccg tcagctacgc tttatgtttt
    16981 tgccggatgg cgaagaccct gacacgctgg tacgaaaaga aggtaaagaa gcgtttgaag
    17041 cgcggatgga gcaggcgatg ccactctccg catttctgtt taacagtctg atgccgcaag
    17101 ttgatctgag tacccctgac gggcgcgcac gtttgagtac gctggcactg ccattgatat
    17161 cgcaagtgcc gggcgaaacg ctgcgaatat atcttcgtca ggaattaggc aacaaattag
    17221 gcatacttga tgacagccag cttgaacgat taatgccaaa agcggcagag aacggcgttt
    17281 ctcgccctgt tccgcagcta aaacgcacga ccatgcgtat acttataggg ttgctggtgc
    17341 aaaatccaga attagcgacg ttggtcccgc cgcttgagaa tctggatgaa aataagctcc
    17401 ctggacttgg cttattcaga gaactggtca acacttgtct ctcccagcca ggtctgacca
    17461 ccgggcaact tttagagcac tatcgtggta caaataatgc tgccaccctt gaaaaactgt
    17521 cgatgtggga cgatatagca gataagaata ttgctgagca aaccttcacc gactcactca
    17581 accatatgtt tgattcgctg cttgaactgc gccaggaaga gttaatcgct cgtgagcgca
    17641 cgcatggttt aagcaacgaa gaacgcctgg agctctggac attaaaccag gagctggcga
    17701 aaaagtgatt taacggctta agtgccgaag agcgatcggg aagcccccga cagccgcact
    17761 gagaggcagc ggcaaatata taagtacgcc ctcgtaatta tcgttggcgg taaacaaccg
    17821 ttggatttca gcgttaacgg ctgaaggaca tcgggtcaat cgcccaacac caacctcatg
    17881 aaataagtgt ggataccgtc ttatggagca aaacccgcag tcacagctga aacttcttgt
    17941 cacccgtggt aaggagcaag gctatctgac ctatgccgag gtcaatgacc atctgccgga
    18001 agatatcgtc gattcagatc agatcgaaga catcatccaa atgatcaacg acatgggcat
    18061 tcaggtgatg gaagaagcac cggatgccga tgatctgatg ctggctgaaa acaccgcgga
    18121 cgaagatgct gccgaagccg ccgcgcaggt gctttccagc gtggaatctg aaatcgggcg
    18181 cacgactgac ccggtacgca tgtacatgcg tgaaatgggc accgttgaac tgttgacccg
    18241 cgaaggcgaa attgacatcg ctaagcgtat tgaagacggg atcaaccagg ttcaatgctc
    18301 cgttgctgaa tatccggaag cgatcaccta tctgctggaa cagtacgatc gtgttgaagc
    18361 agaagaagcg cgtctgtccg atctgatcac cggctttgtt gacccgaacg cagaagaaga
    18421 tctggcacct accgccactc acgtcggttc tgagctttcc caggaagatc tggacgatga
    18481 cgaagatgaa gacgaagaag atggcgatga cgacagcgcc gatgatgaca acagcatcga
    18541 cccggaactg gctcgcgaaa aatttgcgga actacgcgct cagtacgttg taacgcgtga
    18601 caccatcaaa gcgaaaggtc gcagtcacgc tgccgctcag gaagagatcc tgaaactgtc
    18661 tgaagtattc aaacagttcc gcctggtgcc gaagcagttt gactacctgg tcaacagcat
    18721 gcgcgtcatg atggaccgcg ttcgtacgca agaacgtctg atcatgaagc tctgcgttga
    18781 gcagtgcaaa atgccgaaga aaaacttcat tacactgttt accggcaacg aaaccagcga
    18841 tacctggttc aacgcggcaa ttgcgatgaa caagccgtgg tcggaaaaac tgcacgatgt
    18901 ctctgaagaa gtgcatcgcg ccctgcagaa actgcagcag attgaagaag aaaccggcct
    18961 gaccatcgag caggttaaag atatcaaccg tcgtatgtcc atcggtgaag cgaaagcccg
    19021 ccgtgcgaag aaagagatgg ttgaagcgaa cttacgtctg gttatttcta tcgccaagaa
    19081 atacaccaac cgtggcttgc agttccttga cctgattcag gaaggcaaca tcggtctgat
    19141 gaaagcggtt gataaattcg aataccgccg tggttacaag ttctccacct acgcaacctg
    19201 gtggatccgt caggcgatca cccgttctat cgcggatcag gcgcgcacca tccgtattcc
    19261 ggtgcatatg attgagacta tcaacaaact caaccgtatt tctcgccaga tgctgcaaga
    19321 gatgggccgt gaaccgacgc cggaagaact ggctgaacgt atgctgatgc cggaagacaa
    19381 gatccgcaaa gtgctgaaga tcgccaaaga gccaatctcc atggaaacgc cgatcggtga
    19441 tgatgaagat tcgcatctgg gggatttcat cgaggatacc accctcgagc tgccgctgga
    19501 ttctgcgacc accgaaagcc tgcgtgcggc aacgcacgac gtgctggctg gcctgaccgc
    19561 gcgtgaagcg aaagttctgc gtatgcgttt cggtatcgat atgaacaccg accacacgct
    19621 ggaagaagtg ggtaaacagt tcgacgttac ccgcgaacgt atccgtcaga tcgaagcgaa
    19681 ggcgctgcgc aaactgcgtc acccgagccg ttctgaagtg ttgcgtagct tcctggacga
    19741 ttaatcggta ggccggatca ggcgttacgc cgcacccggc actagtccct ctgcacaaac
    19801 gccacctttt cggtggcgtt tttatcgccc acgcactacc agcgcctggt ccagctcgcg
    19861 atacgcttca accagtttct ctagtgaaac gcgacttaaa ccgctgggat ttggcagcac
    19921 ccaaatctgc gtcgaaccaa tggtgagcgt ttgtttcccc cactgtgcac cgcgctggct
    19981 gaatccctgt tcatatgctc gtttgcccag aatcgccaac gcctgcggct gataatcttc
    20041 aattttttca atcagcttac gcccacctgc gtgcagctcc tgctttgaaa cttcattggc
    20101 ttgcaccgtt ggacggtcta ccagtttggt gacgccacaa cgataatcca gcagatgctg
    20161 tgcctcctgc ggcttcaact gacggtcggt aaacccggcc tgatatatca ccttccagaa
    20221 gcgatttgct ggatgagcaa agggaaaacc agtcccggcg gatgaaagcc cagggttgat
    20281 accgcaaaac acgacccgta accctggagc caaaatatcc tcaaccatat ttactccttt
    20341 ctaacatcac caggaaaagt ataacggatt gaaaatacat tgtttataaa aacagcaggc
    20401 gcacggtaat ggctggattg cgacacggag ttactttata atccgctacc atggcccctt
    20461 agctcagtgg ttagagcagg cgactcataa tcgcttggtc gctggttcaa gtccagcagg
    20521 ggccaccaga tatagcaaag gctgacgaga aatcgtcagc ctttttcttt ttatatatca
    20581 gttactttgc atgccagtaa gccgctgcac gtacccgctg tgggtcatac tgttccgctt
    20641 caaagcggcg gcttaaattc ttaacgactt taccttcgcc ggttatccag atgaagtaat
    20701 catcggcagg gatttgcatc tgcgccagac gcgcatctgc cgcctgctca tcatgtgcca
    20761 gccattcgat attaaaacca tcaaggtgcg cgagataatc ctgacaggcg ttatcccgca
    20821 cgctaaccag cgcactaact tgcggtttaa cggcaagttt gctcaacgtt tccaggcggc
    20881 ggcgcagtgc gggcattccg gattcatcgc agacatacag ctgatacgcg taatcttccg
    20941 gcaccaccag cgaaccgcgc ggacctgcca ccgtaagttt atcgcccggt tgcgcctgca
    21001 tcgcccagcc gctggcgacc ccaccgtcgt gaataaagaa atcgatcgcc agttcatggc
    21061 gtagttcgtc atacagcggc gtatagtcac gcgacggtgg gcgtggtcct tccggccaga
    21121 cgatgccctc ttccgttacc gttggcggca caaagtgagc gtcaggttga ggaaagaaga
    21181 gtttgctgtg atcgtcaaag ccgcgcgatg taaaaccgtc cagcgcctcg ccgccgagga
    21241 caatgcgctg aaaaccggcg ctgatgcgct caacgcgtaa cacagtcagt tcacggaagc
    21301 gcagatcatt gcgaacgcgc tgcgggtagc ggagggagtt attcattgtt atcgccttct
    21361 tgatagtaat cagatatatc taaataattt tcgtaaatga taatgattgt taatcatgat
    21421 aaatgcaagc gatttgtaga actgatatat ctgaagtatg tgaaggcatt cagtgccata
    21481 gcaatgctgc cggatgcggc gtgaacgtct tatccggcct agatgtgagg tgcttccacg
    21541 tttaaaaatc atttttgtac ttgcagtaat gctcacatca gatataaatt agatatatct
    21601 aatcaagtaa aaggaggctg atatgagcca tcatcacgaa gggtgttgta aacatgaagg
    21661 ccagccacgc catgagggct gctgcaaagg tgagaagtca gaacacgagc actgcggaca
    21721 cggtcaccag catgaacacg gtcaatgctg cggtggtcgc cacggtcgcg gcggcggtcg
    21781 tcggcaacgt ttctttggtc acggtgaatt gcgtctggtg attctggata ttctctcgcg
    21841 cgatgacagc cacggttacg aattgattaa agcgattgag aacctaaccc aggggaatta
    21901 caccccaagc ccgggcgtca tctacccaac gctggatttt ctgcaggagc agtcgctgat
    21961 taccatccgc gaagaggaag gtggtaagaa gcagattgcg ctgaccgaac aaggcgcgca
    22021 gtggctggaa gaaaaccgcg aacaggtgga gatgattgaa gaacgcatca aagcgcgttg
    22081 cgttggcgcg gcgctgcgcc agaacccgca aatgaagcgg gcgctggata attttaaagc
    22141 ggtgctggat ttacgcgtca accagagcga tatcagtgat gcacaaataa aaaagatcat
    22201 tgcggtgatc gaccgcgccg cttttgatat tacgcaactg gattaatcgc cgcatccgcc
    22261 agtggcgcgg tgcaattgcc ggatgcgacg cttgacgcgc cttatccggc ctacacccgc
    22321 tacacacccc gcaggcctga taagatgcgc cagcatcgca tcaggcattg tgcaccaacc
    22381 gccggatccg gcataccgat taatgcagca cagtcaccgc gtcttccagt cggctggcgc
    22441 ggtgtttcac catcgccgac acctgcgcac tctcttccac cagctcggca tttttctggg
    22501 tgatcaggtt aagctcatcc actgcacggg tcaggctgga aagcccatcg gcctgttcca
    22561 gcgttgaatg gctaatctgg gcgatcaact gggtgacgtt tttcacctgt gccacaatat
    22621 cttccatcgt ccgtccggcg gcgtgtacct gctgcgaacc ggattgcacc ttatcagcac
    22681 tggcatcaat cagcttgcga atatcgttgg cagcattagc gctgcggctg gctaaatgac
    22741 gcacttcccc tgccaccact gcaaaacctt tgccctgttc gccggcacgc gccgcttcca
    22801 ccgccgcatt cagggccaga atattggtct gaaacgcaat atcgttaatc agcgaagtaa
    22861 tggtgccaat gcgctgggta ctgtcggcaa tatcgtccat tgtcttgatc accgtagtca
    22921 tcgcctcccc gccctgcact gccgcattac tggcggtgat agaaagttta tcggcagccg
    22981 acgccgtggc agagttctgt ttcaccgacg ccgccatttg gttcatggtc gccaccgttt
    23041 gctgaacgtt atcaactgtc tgctgggtat gttcgttcag ttcatcggtg cctttcgcca
    23101 gcgtctcact gccatttctg acactggaca cctggcttga gacatcgtta attagccaac
    23161 ggcacatcag gccaagctgc cctaccgcgc gtaatgtcag ccccagctca tcgctgcgat
    23221 tcaggtgctc aacactatta cgctctccgg tcgccacctt cagtgcctga cgggcgacat
    23281 tttctatcgg gcgcacaatc tgccattcaa aacaagcgct tgccaacaat accactaaca
    23341 cacagaggaa atacgtcacc accggggcag caacaaacca aagcatggcc gccagcaaga
    23401 taaacatcag ggtcatcacg ccacgcgccc gccagcgaag cggtaatgaa ggcagtttac
    23461 ccagccagcc tttacgcacc accaggcctt tatgaatacg cttactggta cgtccggagt
    23521 tcaacgcttt gtacagcggc tccaccgccg cgatctcttc atccgtcgcc cgggtacgaa
    23581 tcgacatata gccactgatt tttccctcgc gcaccatcgg taccgcattg gcccgcaccc
    23641 aataatggtc gccatttttg cggcgatttt tcacgatgcc gctccagggc tccccttttt
    23701 tcagggtgaa ccacatatcc gcaaacgccg cttttggcat atccgggtga cgcaccatgt
    23761 tgtgcggctg cccttgtaac tcttgcaagg taaagccgct cacctgcaca aaagtgtcat
    23821 tagcatgagt gatatagctt tgcagatcgg tagtggacat cagagtggta tcgtccgcca
    23881 gcggggtatt ttgctgggtg acatacagat gagaagacat gttcgcgtcc tgtgcaggtt
    23941 atatggttgt taacttcttg tcagagttta tgtcggcccc gctgcggtta tctttaaccg
    24001 attaatttga tttagatcgc aatttgcgat taaagcacaa atctaattct ttgatttaaa
    24061 atactttcac tctgttacta tacgaaaacg ttaattatct tgcccaaaaa tcaggcaatt
    24121 attgccctga aaacgtgcat ttgcgcagca atcatcaaat ccatacccga caaaaaccgt
    24181 gcaaaataac aacaaatgtt aacagatagc attaaatatt gcgcaaatga taaccgcatt
    24241 tgtgtttatc ccgattttcg cgatcgcagc cggagtggcg caatccctgc aatacttaaa
    24301 tcggtatcat gtgatacgcg agcctccgga gcatattttg aacaggttac cttcgagcgc
    24361 atcggcttta gcgtgcagcg cccacgccct gaatctcatt gagaagcgaa cgctggatca
    24421 tgaggagatg aaagcactta accgagaggt gattgaatac ttcaaagagc atgtcaatcc
    24481 ggggttttta gagtatcgca aatctgttac cgccggcggg gattacggag ccgtagagtg
    24541 gcaagcggga agtttaaata cgcttgtcga cacccaggga caggagttta tcgactgcct
    24601 gggaggtttt ggaattttca acgtggggca ccgtaatcca gttgtggttt ccgccgtaca
    24661 gaatcaactt gcgaaacaac cgctgcacag ccaggagctg ctcgatccgt tacgggcgat
    24721 gttggcgaaa acccttgctg cgctaacgcc cggtaaactg aaatacagct tcttctgtaa
    24781 tagcggcacc gagtccgttg aagcagcgct gaagctggcg aaagcttacc agtcaccgcg
    24841 cggcaagttt acttttattg ccaccagcgg cgcgttccac ggtaaatcac ttggcgcgct
    24901 gtcggccacg gcgaaatcga ccttccgcaa accgtttatg ccgttactgc cgggcttccg
    24961 tcatgtgccg tttggcaata tcgaagccat gcgcacggct cttaacgagt gcaaaaaaac
    25021 cggtgatgat gtggctgcgg tgatcctcga accgattcag ggtgaaggtg gcgtaattct
    25081 gccgccgccg ggctatctca ccgccgtacg taagctatgc gatgagttcg gcgcactgat
    25141 gatcctcgat gaagtacaaa cgggcatggg gcgcacgggc aagatgttcg cctgcgagca
    25201 tgagaacgta cagccggata tcctctgcct tgccaaagcg ctcggcggcg gcgtgatgcc
    25261 gattggcgcg accatcgcca ctgaagaggt gttttcagtt ctgttcgaca acccattcct
    25321 gcataccacc acctttggcg gcaacccgct ggcctgtgcg gcggcgctgg cgaccatcaa
    25381 tgtgttgctg gagcagaact taccggctca ggctgatcaa aaaggcgata tgttgctgga
    25441 cggtttccgt caactggcgc gggaatatcc cgatctggta caggaagcgc gtggtaaagg
    25501 gatgttgatg gcgattgagt ttgttgataa cgaaatcggc tataactttg ccagcgagat
    25561 gttccgccag cgcgtactgg tggccggaac gctcaataac gccaaaacga tccgcattga
    25621 accgccactg acactgacca ttgaacagtg tgaactggtg atcaaagcgg cgcgtaaggc
    25681 gctggcggcc atgcgagtaa gtgtcgaaga agcgtaatat caaatcggat ggcgatacga
    25741 cgtcgccatc cgatcttttt atacgacgcg tacgcccgca ggcatcatcc gctccggcgt
    25801 taacagcacg ctttcactgc cgtcatcggt ttccgcacac aacagcatgc attccgacgt
    25861 ttcaccacgc attttcgctt tttgcagatt gcataagacc accaccgttt ttcccatcag
    25921 ctcctcttcg ctgtagtacg gcaccaggct ggtcacggtt tgcagtgttt tttcccccac
    25981 atcaacctgt acgatgtaca gcttgtcggc gttttcatgg cgtttcactt ccacaatctt
    26041 tccgacgcgc atttccagac gtgcaaaatc agcgtaagcc acggtttcca ttgcttcctc
    26101 ccttcaagta aaattttact aaactatagc aaaagttttt ctcagtcctg taggctaaaa
    26161 atggagaatg caggcgtgat cacattcgta agccgctgtg ttaccgttac agcgtcaaag
    26221 aaacgcgctt tatttactga aaacaggtga ccccataagc acttcctcta caatgggggc
    26281 gcacatcagg gaaagtaaaa aaggtaaaca tggcaacact aaaagacatc gcaatcgaag
    26341 ctggcgtatc cctggcgaca gtatccaggg tcttaaatga cgatccgaca ttgaatgtga
    26401 aagaagagac gaaacatcgc attctcgaga tcgccgaaaa gctggagtac aagaccagta
    26461 gtgcccgtaa actccagaca ggcgcagtca accaacacca tattctggct atctacagct
    26521 accagcagga gcaggagatc aacgatcctt actatctggc gatccgccac ggcattgaaa
    26581 ctcagtgcga aaagctggcg atcgaactga ccaactgtta tgaacacaac ggattaccag
    26641 acattaaaaa cgtcaccggt attttaattg tcggcaaacc cacgcccgcc ctgcgcgccg
    26701 ctgccagcgc gttgaccgac aatatctgtt ttatcgactt tcacgaaccc ggcagcggtt
    26761 acgatgcggt ggatatcgat ctggcacgca tcagtaaaga aatcatcgac ttctatatca
    26821 accagggcgt taatcgtatt ggttttattg gcggtgaaga tgagcctggc aaggcggata
    26881 ttcgtgaggt cgcctttgcg gaatatggcc gactgaaaca agtggtacgc gaagaggata
    26941 tctggcgcgg cggtttttcc agttcgtcgg gttatgaact ggcaaaacaa atgctggcgc
    27001 gggaagacta tccgaaggca ctgtttgttg cttccgattc cattgctatc ggcgtactgc
    27061 gggcaattca tgaacgtggc ctgaacatcc cacaggatat ttcgcttttc agcgttaacg
    27121 atatccccac cgcgcgattt acctttccgc cgctctccac cgtgcgcatc cattccgaga
    27181 tgatgggaag tcagggcgtt aacctggtgt atgaaaaagc ccgcgatggt cgcgcgctgc
    27241 cgctgctagt cttcgttccc agcaaattaa aactgcgcgg cacgacccgt taaatcccct
    27301 tacacactgt ccggcaatcg tttttgccgg acagtgctgc cgtttatttt cgtgatccag
    27361 ttaaagtaaa tgcatttacc tgctactttt tagtaaaaat tttactaaac tccccagcaa
    27421 ttacacaaac taccatcacc atgaatggtt ccgatttctc tctaccggga ggccctatga
    27481 atcgctggga aaacattcag ctcacccacg aaaaccgact tgcgccgcgt gcgtactttt
    27541 tttcatatga ttctgttgcg caagcgcgta cctttgcccg cgaaaccagc agcctgtttc
    27601 tgcccttaag cggtcagtgg aatttccact tttttgacca tccgctgcaa gtaccagaag
    27661 ccttcacctc tgagttaatg gctgactggg ggcatattac cgtccccgcc atgtggcaaa
    27721 tggaaggtca cggcaaactg caatataccg acgaaggttt tccgttcccc atcgatgtgc
    27781 cgtttgtccc cagcgataac ccaaccggtg cctatcaacg tattttcacc ctcagcgacg
    27841 gctggcaggg taaacagacg ctgattaaat ttgacggcgt cgaaacctat tttgaagtct
    27901 atgttaacgg tcagtatgtg ggtttcagca agggcagtcg cctgaccgca gagtttgaca
    27961 tcagcgcgat ggttaaaacc ggcgacaacc tgttgtgtgt gcgcgtgatg cagtgggcgg
    28021 actctaccta cgtggaagac caggatatgt ggtggtcagc ggggatcttc cgcgatgttt
    28081 atctggtcgg aaaacaccta acgcatatta acgatttcac tgtgcgtacc gactttgacg
    28141 aagcctattg cgatgccacg ctttcctgcg aagtggtgct ggaaaatctc gccgcctccc
    28201 ctgtcgtcac gacgctggaa tataccctgt ttgatggcga acgcgtggtg cacagcagcg
    28261 ccattgatca tttggcaatt gaaaaactga ccagcgccag ctttgctttt actgtcgaac
    28321 agccgcagca atggtcagca gaatcccctt atctttacca tctggtcatg acgctgaaag
    28381 acgccaacgg caacgttctg gaagtggtgc cacaacgcgt tggcttccgt gatatcaaag
    28441 tgcgcgacgg tctgttctgg atcaataacc gttatgtgat gctgcacggc gtcaaccgtc
    28501 acgacaacga tcatcgcaaa ggccgcgccg ttggaatgga tcgcgtcgag aaagatctcc
    28561 agttgatgaa gcagcacaat atcaactccg tgcgtaccgc tcactacccg aacgatccgc
    28621 gtttttacga actgtgtgat atctacggcc tgtttgtgat ggcggaaacc gacgtcgaat
    28681 cgcacggctt tgctaatgtc ggcgatatca gccgtattac cgacgatccg cagtgggaaa
    28741 aagtctacgt cgagcgcatt gttcgccata ttcacgcgca gaaaaaccat ccgtcgatca
    28801 tcatctggtc gctgggcaat gaatccggct atggctgtaa catccgcgcg atgtaccacg
    28861 cagcgaaggc gctggatgac acgcgactgg tgcattacga agaagatcgc gatgctgaag
    28921 tggtcgatat tatttccacc atgtacaccc gcgtgccgct gatgaatgag tttggtgaat
    28981 acccgcatcc gaagccgcgc atcatctgtg aatatgctca tgcgatgggg aacggaccag
    29041 gcgggctgac ggagtaccag aacgtcttct ataagcacga ttgcattcag ggacattatg
    29101 tctgggaatg gtgcgaccac gggatccagg cgcaggatga caacggcaac gtctggtata
    29161 aattcggcgg cgactacggc gactatccca acaactataa cttctgtctt gatggtttga
    29221 tctattccga tcagacgccg ggaccgggcc tgaaagagta caaacaggtt atcgcgccgg
    29281 taaaaatcca cgcgctggat ctgactcgcg gcgagctgaa agtcgaaaat aaactgtggt
    29341 ttaccacgct tgatgactac accctgcacg cagaggtgcg cgtcgaaggt gaaacgctcg
    29401 cgacgcagca gattaaactg cgcgacgttg cgccgaacag cgaagccccc ttgcagatca
    29461 cgctgccgca gctggacgcc cgcgaagcgt tcctcaacat tacggtgacc aaagattccc
    29521 gcacccgcta cagcgaagcc gggcattcta tcgccactta tcagttcccg ctgaaggaaa
    29581 acaccgcgca accagtgcct ttcgcaccaa ataatgcgcg tccgctgacg ctggaagacg
    29641 atcgtttgag ctgcaccgtt cgcggctaca acttcgcgat caccttctca aaaatgagtg
    29701 gcaaaccgac atcctggcag gtgaatggcg agtcgctgct gacccgcgag ccaaagatca
    29761 acttcttcaa gccaatgatc gacaaccaca agcaggagta cgaagggctg tggcaaccga
    29821 atcatttgca gatcatgcag gaacatctgc gcgactttgc cgtagaacag agcgatggtg
    29881 aagtgttgat catcagccgc acagttattg ccccgccggt gtttgacttc gggatgcgct
    29941 gcacctacat ctggcgcatc gctgccgatg gccaggttaa cgtggcgctt tccggcgagc
    30001 gttacggcga ctatccgcac atcattccgt gcatcggttt caccatgggg attaacggcg
    30061 aatacgatca ggtggcgtat tacggtcgtg gaccgggcga aaactacgcc gacagccagc
    30121 aggctaacat catcgatatc tggcgcagca ccgtcgatgc catgttcgag aactatccct
    30181 tcccgcagaa caacggcaac cgtcagcatg tccgctggac ggcactgact aaccgccacg
    30241 gcaacggtct gctggtggtt ccgcagcgcc caattaactt cagcgcctgg cactataccc
    30301 aggaaaacat ccacgctgcc cagcactgta acgagctgca gcgcagtgat gacatcaccc
    30361 tgaacctcga ccaccagcta cttggcctcg gctccaactc ctggggcagc gaggtgctgg
    30421 actcctggcg cgtctggttc cgtgacttca gctacggctt tacgttgctg ccggtttctg
    30481 gcggagaagc taccgcgcaa agcctggcgt cgtatgagtt cggcgcaggg ttcttttcca
    30541 cgaatttgca cagcgagaat aagcaatgag gatcatcgat aacttagaac agttccgcca
    30601 gatttacgcc tctggcaaga agtggcaacg ctgcgttgaa gcgattgaaa atatcgacaa
    30661 cattcagcct ggcgtcgccc actccatcgg tgactcattg acttaccgcg tggagacaga
    30721 ctccgcgacc gatgcgctat ttaccgggca tcgacgctat tttgaagtgc attactacct
    30781 gcaagggcag caaaaaattg aatatgcgcc gaaagagaca ttacaggtag tggaatatta
    30841 tcgtgatgaa actgaccgtg aatatttaaa aggctgcgga gaaaccgttg aggtccacga
    30901 agggcaaatc gttatttgcg atatccatga agcgtatcgg tttatctgca ataacgcggt
    30961 caaaaaagtg gttctcaaag tcaccatcga agatggttat ttccataata aataacaact
    31021 acggcggcaa aaggagtttg ccgccaccgc taccctactc attttcggag atgtgttatg
    31081 tctgatacca aacgtaatac aatcggcaaa ttcggcttgc tctcgctgac ttttgccgcc
    31141 gtttacagct ttaacaacgt tatcaataat aatattgagc ttggactggc ctcggcaccg
    31201 atgtttttcc tcgcgacgat tttttatttt attcccttct gtctgatcat cgcagaattt
    31261 gtttcgttaa ataaaaactc agaagccggt gtctacgcgt gggtaaaaag ttcgctgggc
    31321 ggacgttggg catttattac tgcctacact tactggttcg taaatctgtt ctttttcacc
    31381 tcgctgttgc cgcgcgttat tgcttatgct tcgtatgcct tcctcggcta cgaatatatt
    31441 atgacgccgg ttgccaccac cgttatcagt atggtgctgt tcgccttctc cacctgggtt
    31501 tccaccaacg gggcgaaaat gctggggcca attacctccg tcacttcaac gctgatgctg
    31561 ctgttaacgc tctcctacat tttactggca ggtacggcac tggttggcgg cgtacagcct
    31621 gccgatccga tcaccgttga cgcgatgatc ccgaacttca actgggcgtt cctcggcgtt
    31681 accacctgga tctttatggc cgcaggtggc gcggagtccg tcgcggtgta cgttaacgac
    31741 gtcaaaggcg gttcgaaatc gttcgttaaa gtgatcatcc tcgccgggat ttttatcggc
    31801 gtactgtatt ccgtctcctc ggtgctgatt aacgtcttcg tcagcagcaa agagttgaaa
    31861 tttaccggcg gatcggtgca agtattccac ggcatggcgg cgtattttgg tctgccggaa
    31921 gcgttgatga atcgctttgt cggtctggtg tcctttaccg cgatgttcgg ctccctgctg
    31981 atgtggaccg caacaccggt gaaaattttc ttctccgaaa tcccggaagg catctttggt
    32041 aagaaaaccg tcgaacttaa cgaaaacggc gttccggcgc gcgcggcgtg gatccagttc
    32101 ctgatcgtca tcccgctgat gattatcccg atgctcggtt ccaataccgt gcaggatctg
    32161 atgaatacta ttattaatat gaccgccgca gcgtccatgc ttccgccgtt attcatcatg
    32221 ctggcttacc tgaatttacg cgccaaatta gatcacctgc cacgcgattt ccgtatgggc
    32281 tcccgccgca ccggtattat cgttgtttca atgctgattg cgatatttgc cgtagggttt
    32341 gtcgcttcga cattcccgac tggcgcgaat attctgacca tcatttttta taacgtcggc
    32401 ggtattgtta tcttccttgg ctttgcgtgg tggaaataca gtaaatatat aaagggatta
    32461 acggctgaag agcgccatat tgaagcgacg ccagccagca atgttgatta agcagaataa
    32521 taaaaaatga gcaggcattt tccctcttcc tggatgagga gagggaaaat agtttctgcc
    32581 tttatatttt tataattaca acgataaaag gctgtacttt ttctttagct catggattaa
    32641 cacaatgaaa ttaatcactg caccatgcag agcattactt gctctgccgt tttgctacgc
    32701 cttttctgcg gcaggagaag aagcacgtcc ggcagaacat gacgacacaa aaacacctgc
    32761 aattacctcg acatcttctc cttcatttcg tttttacggc gaattagggg ttggtggata
    32821 tatggattta gagggtgaga ataaacataa atacagcgac ggtacctata ttgaaggtgg
    32881 cctggagatg aagtacggct cctggttcgg cctgatttac ggcgaaggct ggaccgtgca
    32941 ggccgaccac gacggcaatg cctgggtgcc agaccatagc tggggtggtt tcgagggcgg
    33001 aattaaccgt ttctatggcg gttatcgtac caatgatggc accgaaatca tgctcagtct
    33061 gcgtcaggat tcctcgctgg atgacctgca atggtggggc gatttcaccc ccgatctggg
    33121 ctacgtcatt cccaataccc gcgacattat gactgcgctg aaggtacaga acttaagcgg
    33181 caactttcgt tatagcgtca ccgcgactcc tgccggacat catgatgaaa gcaaagcctg
    33241 gctacatttt ggcaaatacg atcgctatga cgacaaatac acctatccgg caatgatgaa
    33301 cggttacatc cagtatgacc ttgccgaagg catcacctgg atgaacggtc tggaaatcac
    33361 cgacggcaca ggacagctct atctcacggg cctgctaatt cctaactttg ccgctcgcgc
    33421 ctggcaccat accggacgcg ccgacgggct ggacgtaccg ggaagtgaaa gtgggatgat
    33481 ggtgagcgcc atgtatgaag cgttaaaggg cgtttatctc tccaccgctt acaccaacgc
    33541 caaacatcgc cctgaccacg ctgacgatga aaccacctct ttcatgcagt ttggtatctg
    33601 gtacgaatac ggcggcggac gtttcgccac ggcttttgat agccgcttct acatgaaaaa
    33661 tgcctctcac gatcccagcg accaaatctt cctgatgcaa tatttctact ggtaataagg
    33721 actgtaattt catgaaaata aaaactattt taacgccagt aacctgcgct ctgctgataa
    33781 gtttttccgc ccatgccgct aacgccgaca attataaaaa cgtgattaac cgtactggcg
    33841 cgccgcagta catgaaggat tacgattacg acgatcacca gcgttttaat ccgtttttcg
    33901 atctcggagc ctggcatggt catctgttgc cagacggccc taacaccatg ggcggctttc
    33961 cgggcgttgc gctgctgacg gaagagtaca tcaactttat ggccagcaat ttcgaccgcc
    34021 tgaccgtctg gcaggacggc aagaaagtcg actttacgct ggaggcatac agtattcccg
    34081 gtgcgctggt gcaaaaactg acagcaaaag atgtgcaagt cgaaatgact ctgcgcttcg
    34141 ccacgccgcg cacgtcacta ctggaaacca aaatcaccag caataaaccg ctggatctgg
    34201 tgtgggacgg cgaactgctg gaaaaactgg aagcgaaaga agggaaaccg ctttccgata
    34261 aaaccattgc tggcgaatac cctgactatc agcgcaaaat cagcgccact cgtgatggcc
    34321 tgaaagtcac ctttggcaaa gtgcgcgcca cctgggatct gctgacctcc ggcgaatcgg
    34381 aatatcaggt gcataaatcc ctgccggtgc agactgaaat caacggtaat cgctttacca
    34441 gtaaggcgca tatcaacggt tcgaccacgc tctacaccac ctattcccat ctgctgaccg
    34501 ctcaggaagt tagcaaagag caaatgcaga tccgcgatat tctggcgcgt ccggcgtttt
    34561 atctcaccgc ctcgcagcaa cgctgggaag aatacctgaa gaaagggtta accaatccgg
    34621 atgcgacgcc ggaacagacg cgcgtcgcgg tgaaagcgat cgaaacgctc aacggtaact
    34681 ggcgctcacc gggcggtgcg gtgaaattta acaccgttac accgtcggtg accgggcgct
    34741 ggttctccgg caatcagacc tggccgtggg atacctggaa gcaggcgttt gcgatggcgc
    34801 atttcaatcc ggacatcgcc aaagagaata tccgcgcggt cttctcctgg cagatccagc
    34861 ctggcgaccg cgtgcgtccg caggatgtgg gctttgtccc cgacctgata gcgtggaacc
    34921 ttagccccga gcgtggtggc gatggcggca actggaacga acgtaatacc aaacccagcc
    34981 ttgccgcctg gtcggtgatg gaagtgtaca acgtcaccca ggataaagcc tggttggcag
    35041 agatgtaccc gaaactggtg gcctatcacg actggtggtt acgtaaccgc gatcacaacg
    35101 gcaacggcgt gccggaatat ggcgcgaccc gcgacaaagc ccacaacact gagagcggcg
    35161 agatgctgtt tacggtgaag aaaggcaaca aagaagagac gcagtctggg ctgaacaact
    35221 acgcccgcgt ggtggagaaa ggccagtacg acagtctgga aattccggca caggtcgctg
    35281 cgtcgtggga atcggggcgt gatgatgccg ccgtctttgg ttttatcgac aaagaacagt
    35341 tggataaata tgtcgctaat ggcggcaaac gtagcgactg gacggtgaaa ttcgccgaaa
    35401 accgcagtca ggacggaacg ttgctgggct actcgctatt gcaggagtcg gtggatcagg
    35461 ccagctatat gtacagcgat aaccattatc tggcggagat ggcaaccatc ctcggtaagc
    35521 cagaagaggc caaacgctat cgccagttgg cacagcagct cgcggactac atcaacacct
    35581 gtatgttcga cccgactacc cagttctact atgacgtacg tattgaagat aaaccactgg
    35641 cgaacggctg cgcgggcaaa ccgattgttg agcgcggtaa agggccggaa ggctggtcgc
    35701 cgctgtttaa cggtgcggca acgcaggcca atgccgacgc ggtggtgaag gtgatgctcg
    35761 atcctaaaga gttcaacacc tttgtcccgc tgggaacggc ggcgttaacc aatccggctt
    35821 ttggcgctga tatctactgg cgcgggcgcg tatgggtgga tcagttctgg tttggtctga
    35881 aagggatgga gcgctacggt tatcgtgatg atgccctgaa gctggcggat acgttcttcc
    35941 ggcacgccaa aggattaacc gccgatggcc caattcagga gaattacaac ccgctgactg
    36001 gcgcgcagca aggcgcacca aatttctcct ggagtgccgc gcatttgtat atgttgtata
    36061 acgatttttt ccgtaagcaa taatttgcaa ctgctggcgg atgcggcgta aacgccttat
    36121 ccgccctaca tgtgcaatcc tcctggccgg ataaaacgcg gcaagcgttg catccggcaa
    36181 ctgcaccgcg ccactggcgg atgcggcgtg aacgccttat ccgccctaca tgtgcaatcc
    36241 cccaggccgg ataaaacacg gcaagcgtcg catccggcgt taccaccggg cgtattcttt
    36301 ttgaatccca tcacaaaccc cgcactcccc ttttcccttt tctccggcga cggctaaatt
    36361 agaactcatc cgaccacata acaattattt tacatactgg acaattttat gagctacccg
    36421 tcgctgttcg ccccgctgga tttaggtttt accacgttaa aaaaccgcgt gttgatgggc
    36481 tcaatgcaca ccgggctgga ggaatacccg gacggtgccg agcggctggc agcgttttat
    36541 gccgaacgcg cccgtcacgg cgtggcgctg attgtcagcg gcggcattgc accagattta
    36601 acaggcgttg gcatggaagg tggcgcaatg ctcaacgacg ccagccagat cccacaccat
    36661 cgcaccatta ccgaagcggt acatcaggaa ggcggcaaaa tagccctgca aattttgcat
    36721 accgggcgct acagctacca accgcatctg gtcgctccct ccgcattgca ggcccccatc
    36781 aaccgtttcg ttcctcacga actcacccat gaagagatcc tgcaactgat cgacgatttc
    36841 gcccactgcg cgcaactggc acgggaggca ggatacgacg gtgtagaggt aatggggtcc
    36901 gaagggtatt tgatcaacga atttctgacg ctgcgcacca atcagcgtag tgaccagtgg
    36961 ggcggcgatt accgcaaccg gatgcgattt gccgtagaag tagtgcgtgc ggtgcgcgaa
    37021 cgcgtcggca acgacttcat tattatctac cgactgtcga tgctcgacct ggtcgaagac
    37081 ggcgggactt ttgccgaaac ggtagagctg gcgcaggcca ttgaagcggc gggcgcgacc
    37141 attatcaaca ccggcattgg ctggcatgaa gcacgtattc cgaccattgc cacgcccgtg
    37201 ccgcgcggcg catttagctg ggtcacgcgc aaactgaaag gccacgtctc gctgccgcag
    37261 gtaaccacca accggattaa cgatccgcag gttgccgacg atattctctc gcgcggcgat
    37321 gccgatatgg tatcgatggc acgaccgttt cttgctgatg cggaactgct gtcaaaagcg
    37381 caatcggggc gagccgatga gatcaacacc tgcattggct gcaatcaggc ctgcctcgat
    37441 cagatcttcg ttggcaaagt cacctcgtgc ctggtgaatc ctcgcgcctg ccacgaaacc
    37501 aaaatgccaa tccttcctgc cgtgcagaaa aaaaatctgg cggtggtcgg tgcgggacct
    37561 gctgggctgg cgtttgccat taacacggcg gcgcgtgggc atcaggtaac attgtttgat
    37621 gcccacagcg agatcggcgg gcagtttaat atcgccaaac agatccccgg caaagaggag
    37681 ttttacgaaa cgctgcgcta ttaccatcgg atgatcgaag tgacgggcgt gacgctgaaa
    37741 ctcaatcaca ccgtgacggc ggatcagtta caggcgttcg atgaaacgat cctcgccagt
    37801 gggatcgtgc cgcgcgttcc gcctatcgac gggatcgatc atccgaaggt attgagttat
    37861 ctcgatgtac tgcgcgacaa agcgccggta ggcaacaagg ttgccatcat cggttgtggc
    37921 gggattggtt ttgatacggc gatgtattta agtcagccgg gcgaatccac cagccagaat
    37981 atcgccaggt tctgtaatga gtgggggatc gacagcagcc tacaacaggc tggtggctta
    38041 agcccgcagg gaatgcagat cccgcgtagc ccacggcaaa ttgtgatgct ccagcgcaaa
    38101 gccagcaaac cgggacaggg gttaggcaaa accaccggct ggatccatcg caccaccctg
    38161 ctctcgcggg gtgtgaaaat gatcccaggc gtaagttatc agaagattga cgatgacggg
    38221 ctgcatgtgg tgatcaacgg cgaaacgcag gtattagcag tggacaatgt ggtgatctgc
    38281 gcagggcaag agccaaaccg cgcgctggcg caaccgctga ttgatagcgg gaaaactgtg
    38341 catttaattg gcggctgcga tgtggctatg gagctggacg cacgacgggc aattgcccag
    38401 ggaacacggc tggcgctgga gatttaaatc gttatttgaa gcgccggatg caacgcatcc
    38461 ggcacgtcgc gttaatcaat aaacaaggcg ggagaaatgc cgaatcgcgt tgccaatttt
    38521 ttagcgtgtt ccagcgttaa tttcctcttc ccgctcaaaa cgcgtgacac catagattta
    38581 ctgccaattt ccggcagatc ggaaagggtt aaaccatatt gatccataag ggtacgaatc
    38641 acggctatac cgccaggcat ggcttgagcc atggcattaa attccgcaaa ttcgggcgct
    38701 gattcttccc acgcggttat tttggcacac accagatcca gcaaggggtt ttcaggatcg
    38761 ttgagcagca gatgatctac cagttccagc gcctgggtgt attgttcctc gttctgaata
    38821 cccgccagaa aaggtgccac agcagttagc ttttctcctg cttgcaagat gtcggcaatc
    38881 gcaatcattt tttcccctta gtacgatgaa cagcggtaaa gaaatcgtat tctttatgcg
    38941 tcataacttc acgtatgtag cacttttgcg attcaaaaaa gaccattgct acaacacgta
    39001 attcattgcc cccaacattg aaaacataat gcttatccag atatttgaag ttatccagag
    39061 atgggaatac tgcttttaat gactcaggtt ttttgaaata tcccttagca atcgtgttcc
    39121 ccagagccac aaactccgtt ttatgttgcg ggtatttttc cgcagcatct ttcaatgctt
    39181 tttgagttat caggtgcatt cttcatcacg tccgttgaca aattggcaat atgataacat
    39241 ccgttgccag attggcaacg gatgaattta tctgtgtgac ggaagaataa acgtagaaaa
    39301 acgctaaaca agagaatgta gaggatggtg agagggattt tttgttttat ggaagcgcga
    39361 ttccagaact gggcagacat tgccggatgc gacgcttgcc gcgtcttatc cggcctggga
    39421 gtaacgtgct gaacgtaggc cggataaggc gttaacgccg catccggcag gtaactccca
    39481 atcttaccga cgacgcccca gcttcactgc tttcagcacc acaaatttat tattcgtggc
    39541 gatggtggtg cagttgccga aaatcttctt cagtttatgg aagtaatcca ggtgacggtt
    39601 ggcaacaata tacagctcgc cgttgatttt caggcagcgg cgggcatggt ggaacatctc
    39661 ccaggcgacg ttatcggtca gcgcatgttg ttggtgaaac ggcgggttgc agagcacagc
    39721 attaaagcgg aaaggctcca cgccggagag cgcgttgttg atcataaact cgctgcgatc
    39781 caacgcctct ggcatgttgg tttcaacgtt caaacggctg gaagcaaccg ccatcggcga
    39841 ttcatcgaca aacaccactt tcgcctgcgg gtttttatca agcagcgtca gaccaataac
    39901 gccattaccg caaccgagat cgacaatctc cccttcgaga ttctctggca gatgttgcat
    39961 aaagaagcgc gcgccgatat caagcccggt gcgggagaag acattcgcat ggttatggat
    40021 agtccagtca gtaccttcca gcttccagct aacggtctgc ggcgcatcgg ccagcggcgg
    40081 ttcattgaac gtgcaattaa tcaggcgcgc ttttttccat gccagcgtgg tggtggtcgg
    40141 gccgagcact ttttcgaaca gttccagcgt ggaagtgtga atgtcacggg ctttggcacc
    40201 ggcaataata cgtgtatccg acgtgaccac tttgcgcagc gcacgcagtt gttgttccag
    40261 caatgccagt gttttcggca ctttgatcag caccacaccc ggctgttgcg ggtagtcggc
    40321 ggtgctgtcg agaaacttca cgctcgactc atcaatcccg ttgaggcgta aattctcgcg
    40381 cgtcgccagt tcactgatgt atgagtcgcc aatgctgtac ggcttatgtt ccgccagcgc
    40441 gcagcttaac gcaccaaagg catcattcag gatcaacacc gggccgcgga tttctgtgtc
    40501 gtccaactgt tgcagcaaat attcatccgc cgcttcccac gcctgtagcg ggttaacgtc
    40561 atccgtcgcc ggaaaacgtt gtagtgtcag tgaacggaaa ccgttgtcta agtggctcat
    40621 cggccctcct gaatgataaa atttcgacgt tgcccctgaa aagggtgcgt gagtataccc
    40681 tttttctctt tttcgtgggc ggttatgagc aatcttactt atcttcaggg ttaccccgag
    40741 cagctacttt cccaggtgcg aacactgatt aacgaacagc gtctgggcga tgtgctggca
    40801 aaacgctatc cgggaacaca cgactacgcc accgataaag ccctctggca atatactcag
    40861 gatctgaaaa atcagtttct gcgtaatgcc ccgccgatca ataaagtgat gtatgacaat
    40921 aagatccatg tgctaaaaaa cgcgctcggg ttacataccg ctgtttctcg tgtgcagggc
    40981 ggcaagctga aagcaaaagc ggagatccgc gtcgctaccg tgtttcgcaa cgcgccggaa
    41041 ccgtttttgc gcatgatcgt ggtgcacgag ctggcgcacc tgaaggagaa agagcataac
    41101 aaagcgttct atcaattgtg ctgccatatg gagccgcagt accaccagct tgagttcgac
    41161 acccggctgt ggctaacgca gttgtcgctt gggcaggaca aaatctgaaa atgatgtcag
    41221 gcgttatctt ttctttcttt agcgcctaaa atcgacctcc cccctttcgt taaggataac
    41281 gaccatgctg cgcgcatttg cccgctttct tctccgtatt tgtttttctc gccggactct
    41341 aaagattgcc tgcttactgt tacttgtggc aggagcgacc atttttatcg ccgatcgagt
    41401 aatggtcaac gccagcaagc agctgacctg gagcgatgtc aacgcagttc cggcgcgtaa
    41461 cgtgggctta ttgctggggg ccaggccggg taatcgctac tttacccgac gtattgatac
    41521 cgcagcagcg ttgtaccacg ccggaaaagt gaaatggcta ctggtaagtg gcgataacgg
    41581 acgtaaaaat tacgatgaag catcaggtat gcagaaggct ttgatcgcta aaggcgtacc
    41641 ggcgaaagtg atcttctgcg actatgccgg attctcaacg ctggattcgg tagtgcgtgc
    41701 caaaaaggtt tttggtgaaa accatatcac cattatctcg caagaatttc ataaccaacg
    41761 cgccatctgg ctggcaaaac agtacagcat cgacgctatc ggttttaacg cgcctgacct
    41821 caatatgaag cacggttttt atactcaact gcgggagaaa ctcgctcgtg tcagcgccgt
    41881 gattgacgcc aaaatcctcc atcgccagcc gaaatatctc gggccatcgg tcatgattgg
    41941 gccgtttagt gagcatggct gtccggcaaa agaataatgc gtatctgcgc acgtcgaaga
    42001 tgaaaaaggc gtgctacatt gacgacagaa tccctttatg gagtatccac gcgttatgat
    42061 acgtttcgct gtgattggta cgaactggat cactcgccag ttcgtcgagg ccgcccatga
    42121 gagcggtaaa tacaagttaa ccgccgtata ttcccgcagc cttgaacagg cccagcactt
    42181 cgccaatgat ttttctgtcg agcatctgtt tacctcgctg gaagcgatgg cggaaagcga
    42241 tgccattgac gcggtgtata ttgccagccc gaatgccctg cacttttccc agacacaact
    42301 tttccttagc cataaaattc atgtgatttg cgagaaacca ctggcgtcga atctggcgga
    42361 agtggatgcc gccattgcct gtgcgcggga aaatcaggtg gtgctgtttg aggcatttaa
    42421 aaccgcctgc ctgccgaact ttcatttgct gcgccaggcg ctgccgaaag tcggcaaatt
    42481 gcgtaaagtc tttttcaact attgccagta ttcctcgcgc tatcaacgtt acctggacgg
    42541 tgaaaatccc aacaccttta atccgtcatt ctctaacggt tcaattatgg atatcggctt
    42601 ttactgcctg gcgtcggcgg tggcgttatt tggtgagccg aaaagcgtgc aggcaaccgc
    42661 cagtttgctg gcaagcggtg tagatgccca cggcgtggtg gtgatggatt acggtgattt
    42721 cagcgtcacc ttgcagcact caaaagtcag tgattctgtc ctggcgagcg agattcaggg
    42781 cgaagcagga tcgctggtga ttgaaaaact gtctgaatgc cagaaagtgt gcttcgtgcc
    42841 gcgtggcagc caaatgcagg atctcaccca gccgcagcat attaatacca tgctctacga
    42901 agcagagctg ttcgctaccc tggtggatga gcatctggtg gatcatccgg ggctggcggt
    42961 cagtcgcatc accgccaaac tgctgaccga gatccgccgc cagactgggg tgatttttcc
    43021 ggcagatagc gtaaaactat aattgccaaa gtaaaacagt gtaaaaggta tgtaacagac
    43081 cattgactgg ctgaatggtc tgtcatactt tgttacctgc aaaggggagt aacttcattg
    43141 ccggtcgatc gtcattacga tgtgtgaaaa accacatccg gtcaccgggc aacccgaaag
    43201 gaatacgcag acgtattcct tttttgttgt aagtgagacc ttgccggaag gcgaggtcta
    43261 tgcataaaaa gcagcggctg acgtcttccg acgttggccg tttttttatg tgtaaggaac
    43321 ttctatgaat actgtcggca cgccgttgct atggggcgga ttcgctgttg ttgtcgccat
    43381 tatgctggct atcgacctgt tgttgcaggg gcgtcgtggg gcacatgcca tgaccatgaa
    43441 acaggctgcg gcctggtcgc tggtctgggt gacgctgtcg ttactgttta acgccgcttt
    43501 ctggtggtat ctggtgcaaa ccgaaggtcg cgccgttgcc gatccacagg cactggcctt
    43561 tctcacaggt tatctgattg agaaatcgct ggcggtcgat aacgtctttg tctggctgat
    43621 gttgttcagc tatttctctg ttccggcggc attacaacgc cgcgtgctgg tgtatggcgt
    43681 gctcggggcg attgttctgc gtaccatcat gatcttcact ggcagctggc taatttcgca
    43741 gttcgactgg atcctgtata tcttcggtgc cttcctgctg tttaccggcg tgaagatggc
    43801 gctcgcccat gaagatgaat caggcattgg cgacaagccg ctggtgcgct ggctacgcgg
    43861 tcatttgcgc atgaccgaca ccatcgacaa cgagcatttc tttgtgcgta agaatggatt
    43921 gctgtacgcc acaccgctga tgctggtact gattctggtg gaattgagcg acgtgatttt
    43981 cgccgtggat agcattccgg ctatcttcgc cgtgaccact gacccgttca ttgtgctgac
    44041 ctcaaacctg tttgcgatcc tcggcctgcg tgcgatgtat ttcctgctgg cgggcgtagc
    44101 agagcgtttc tcgatgctca aatatggcct ggcggtgatc ctggtgttta tcggtatcaa
    44161 gatgctgatt gtcgacttct accatattcc aatcgccgtc tcgctgggcg tggtgtttgg
    44221 cattctggtg atgacgttta ttatcaacgc ctgggtgaat tatcggcatg ataagcagcg
    44281 gggagaatga ttcgctgtat attaagccat tcctagagat aactcattcc agacaaaggt
    44341 gagctattaa ttatgtaatc ggcttgtagc tctttacaaa aagaccgcac gggtgttggt
    44401 ggcgtcaaaa tcgccgaagc gttccctgga ggccggggcg acgcgcatgg atgcgcggcg
    44461 agggcgcatc ttacagggac gttcccttgc gcccgacccg gtagccgcaa gggataagct
    44521 gagggaa
//
LOCUS       DAZKHC020000029        44303 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZKHC020000029 DAZKHC020000000
VERSION     DAZKHC020000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 44303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.029"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(236..1465)
                     /gene="yqeG"
                     /locus_tag="ACHOSM_RS09490"
                     /old_locus_tag="pgaptmp_001898"
     CDS             complement(236..1465)
                     /gene="yqeG"
                     /locus_tag="ACHOSM_RS09490"
                     /old_locus_tag="pgaptmp_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4558437.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATELVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            1720..2901
                     /gene="yqeF"
                     /locus_tag="ACHOSM_RS09495"
                     /old_locus_tag="pgaptmp_001899"
     CDS             1720..2901
                     /gene="yqeF"
                     /locus_tag="ACHOSM_RS09495"
                     /old_locus_tag="pgaptmp_001899"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGU4558438.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVIDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3188..4024
                     /gene="kduI"
                     /locus_tag="ACHOSM_RS09500"
                     /old_locus_tag="pgaptmp_001900"
     CDS             3188..4024
                     /gene="kduI"
                     /locus_tag="ACHOSM_RS09500"
                     /old_locus_tag="pgaptmp_001900"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HGU4558439.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            4054..4815
                     /gene="kduD"
                     /locus_tag="ACHOSM_RS09505"
                     /old_locus_tag="pgaptmp_001901"
     CDS             4054..4815
                     /gene="kduD"
                     /locus_tag="ACHOSM_RS09505"
                     /old_locus_tag="pgaptmp_001901"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HGU4558440.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            5130..6548
                     /gene="araE"
                     /locus_tag="ACHOSM_RS09510"
                     /old_locus_tag="pgaptmp_001902"
     CDS             5130..6548
                     /gene="araE"
                     /locus_tag="ACHOSM_RS09510"
                     /old_locus_tag="pgaptmp_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HGU4558441.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGI"
     gene            6677..7369
                     /gene="ygeA"
                     /locus_tag="ACHOSM_RS09515"
                     /old_locus_tag="pgaptmp_001903"
     CDS             6677..7369
                     /gene="ygeA"
                     /locus_tag="ACHOSM_RS09515"
                     /old_locus_tag="pgaptmp_001903"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HGU4558442.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERRVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(7356..8291)
                     /gene="lysR"
                     /locus_tag="ACHOSM_RS09520"
                     /old_locus_tag="pgaptmp_001904"
     CDS             complement(7356..8291)
                     /gene="lysR"
                     /locus_tag="ACHOSM_RS09520"
                     /old_locus_tag="pgaptmp_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HGU4558443.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            8413..9675
                     /gene="lysA"
                     /locus_tag="ACHOSM_RS09525"
                     /old_locus_tag="pgaptmp_001905"
     CDS             8413..9675
                     /gene="lysA"
                     /locus_tag="ACHOSM_RS09525"
                     /old_locus_tag="pgaptmp_001905"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGU4558444.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            complement(9682..10713)
                     /gene="galR"
                     /locus_tag="ACHOSM_RS09530"
                     /old_locus_tag="pgaptmp_001906"
     CDS             complement(9682..10713)
                     /gene="galR"
                     /locus_tag="ACHOSM_RS09530"
                     /old_locus_tag="pgaptmp_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HGU4558445.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDDDLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            11299..13458
                     /gene="aas"
                     /locus_tag="ACHOSM_RS09535"
                     /old_locus_tag="pgaptmp_001907"
     CDS             11299..13458
                     /gene="aas"
                     /locus_tag="ACHOSM_RS09535"
                     /old_locus_tag="pgaptmp_001907"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HGU4558446.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            13451..14644
                     /gene="lplT"
                     /locus_tag="ACHOSM_RS09540"
                     /old_locus_tag="pgaptmp_001908"
     CDS             13451..14644
                     /gene="lplT"
                     /locus_tag="ACHOSM_RS09540"
                     /old_locus_tag="pgaptmp_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HGU4558447.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(14676..15716)
                     /gene="tas"
                     /locus_tag="ACHOSM_RS09545"
                     /old_locus_tag="pgaptmp_001909"
     CDS             complement(14676..15716)
                     /gene="tas"
                     /locus_tag="ACHOSM_RS09545"
                     /old_locus_tag="pgaptmp_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HGU4558448.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(15824..16042)
                     /gene="ygdR"
                     /locus_tag="ACHOSM_RS09550"
                     /old_locus_tag="pgaptmp_001910"
     CDS             complement(15824..16042)
                     /gene="ygdR"
                     /locus_tag="ACHOSM_RS09550"
                     /old_locus_tag="pgaptmp_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HGU4558449.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            complement(16180..16893)
                     /gene="ygdQ"
                     /locus_tag="ACHOSM_RS09555"
                     /old_locus_tag="pgaptmp_001911"
     CDS             complement(16180..16893)
                     /gene="ygdQ"
                     /locus_tag="ACHOSM_RS09555"
                     /old_locus_tag="pgaptmp_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU4558450.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            complement(16962..17651)
                     /gene="mutH"
                     /locus_tag="ACHOSM_RS09560"
                     /old_locus_tag="pgaptmp_001912"
     CDS             complement(16962..17651)
                     /gene="mutH"
                     /locus_tag="ACHOSM_RS09560"
                     /old_locus_tag="pgaptmp_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HGU4558451.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            17837..17983
                     /gene="ygdT"
                     /locus_tag="ACHOSM_RS09565"
                     /old_locus_tag="pgaptmp_001913"
     CDS             17837..17983
                     /gene="ygdT"
                     /locus_tag="ACHOSM_RS09565"
                     /old_locus_tag="pgaptmp_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HGU4558452.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            18336..18866
                     /gene="rppH"
                     /locus_tag="ACHOSM_RS09570"
                     /old_locus_tag="pgaptmp_001914"
     CDS             18336..18866
                     /gene="rppH"
                     /locus_tag="ACHOSM_RS09570"
                     /old_locus_tag="pgaptmp_001914"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HGU4558453.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            18879..21125
                     /gene="ptsP"
                     /locus_tag="ACHOSM_RS09575"
                     /old_locus_tag="pgaptmp_001915"
     CDS             18879..21125
                     /gene="ptsP"
                     /locus_tag="ACHOSM_RS09575"
                     /old_locus_tag="pgaptmp_001915"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4558454.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            21276..22151
                     /gene="lgt"
                     /locus_tag="ACHOSM_RS09580"
                     /old_locus_tag="pgaptmp_001916"
     CDS             21276..22151
                     /gene="lgt"
                     /locus_tag="ACHOSM_RS09580"
                     /old_locus_tag="pgaptmp_001916"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGU4558455.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            22158..22952
                     /gene="thyA"
                     /locus_tag="ACHOSM_RS09585"
                     /old_locus_tag="pgaptmp_001917"
     CDS             22158..22952
                     /gene="thyA"
                     /locus_tag="ACHOSM_RS09585"
                     /old_locus_tag="pgaptmp_001917"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGU4558456.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            23137..23607
                     /gene="ppdA"
                     /locus_tag="ACHOSM_RS09590"
                     /old_locus_tag="pgaptmp_001918"
     CDS             23137..23607
                     /gene="ppdA"
                     /locus_tag="ACHOSM_RS09590"
                     /old_locus_tag="pgaptmp_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU4558457.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            23598..24161
                     /gene="ppdB"
                     /locus_tag="ACHOSM_RS09595"
                     /old_locus_tag="pgaptmp_001919"
     CDS             23598..24161
                     /gene="ppdB"
                     /locus_tag="ACHOSM_RS09595"
                     /old_locus_tag="pgaptmp_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU4558458.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            24158..24565
                     /gene="ygdB"
                     /locus_tag="ACHOSM_RS09600"
                     /old_locus_tag="pgaptmp_001920"
     CDS             24158..24565
                     /gene="ygdB"
                     /locus_tag="ACHOSM_RS09600"
                     /old_locus_tag="pgaptmp_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HGU4558459.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            24550..24873
                     /gene="ppdC"
                     /locus_tag="ACHOSM_RS09605"
                     /old_locus_tag="pgaptmp_001921"
     CDS             24550..24873
                     /gene="ppdC"
                     /locus_tag="ACHOSM_RS09605"
                     /old_locus_tag="pgaptmp_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGU4558460.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            24886..28254
                     /gene="recC"
                     /locus_tag="ACHOSM_RS09610"
                     /old_locus_tag="pgaptmp_001922"
     CDS             24886..28254
                     /gene="recC"
                     /locus_tag="ACHOSM_RS09610"
                     /old_locus_tag="pgaptmp_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HGU4558461.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGLSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMMRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            28430..31318
                     /gene="ptrA"
                     /locus_tag="ACHOSM_RS09615"
                     /old_locus_tag="pgaptmp_001923"
     CDS             28430..31318
                     /gene="ptrA"
                     /locus_tag="ACHOSM_RS09615"
                     /old_locus_tag="pgaptmp_001923"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HGU4558462.1"
                     /translation="MPRSTWFKALLLFVALWVPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISSKEPHNKTAYFVDAPYQVDKISEQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FNYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEALATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            31311..34853
                     /gene="recB"
                     /locus_tag="ACHOSM_RS09620"
                     /old_locus_tag="pgaptmp_001924"
     CDS             31311..34853
                     /gene="recB"
                     /locus_tag="ACHOSM_RS09620"
                     /old_locus_tag="pgaptmp_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HGU4558463.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVCRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            34853..36679
                     /gene="recD"
                     /locus_tag="ACHOSM_RS09625"
                     /old_locus_tag="pgaptmp_001925"
     CDS             34853..36679
                     /gene="recD"
                     /locus_tag="ACHOSM_RS09625"
                     /old_locus_tag="pgaptmp_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HGU4558464.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMVL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPTGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(36741..38072)
                     /gene="argA"
                     /locus_tag="ACHOSM_RS09630"
                     /old_locus_tag="pgaptmp_001926"
     CDS             complement(36741..38072)
                     /gene="argA"
                     /locus_tag="ACHOSM_RS09630"
                     /old_locus_tag="pgaptmp_001926"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HGU4558465.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDN
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERITAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            38304..39557
                     /gene="amiC"
                     /locus_tag="ACHOSM_RS09635"
                     /old_locus_tag="pgaptmp_001927"
     CDS             38304..39557
                     /gene="amiC"
                     /locus_tag="ACHOSM_RS09635"
                     /old_locus_tag="pgaptmp_001927"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HGU4558466.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            complement(39633..39709)
                     /locus_tag="ACHOSM_RS09640"
                     /old_locus_tag="pgaptmp_001928"
     tRNA            complement(39633..39709)
                     /locus_tag="ACHOSM_RS09640"
                     /old_locus_tag="pgaptmp_001928"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39673..39675),aa:Met,seq:cat)
     gene            39918..41015
                     /gene="mltA"
                     /locus_tag="ACHOSM_RS09645"
                     /old_locus_tag="pgaptmp_001929"
     CDS             39918..41015
                     /gene="mltA"
                     /locus_tag="ACHOSM_RS09645"
                     /old_locus_tag="pgaptmp_001929"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HGU4558467.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            41092..41898
                     /gene="tcdA"
                     /locus_tag="ACHOSM_RS09650"
                     /old_locus_tag="pgaptmp_001930"
     CDS             41092..41898
                     /gene="tcdA"
                     /locus_tag="ACHOSM_RS09650"
                     /old_locus_tag="pgaptmp_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HGU4558468.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(41949..42392)
                     /gene="csdE"
                     /locus_tag="ACHOSM_RS09655"
                     /old_locus_tag="pgaptmp_001931"
     CDS             complement(41949..42392)
                     /gene="csdE"
                     /locus_tag="ACHOSM_RS09655"
                     /old_locus_tag="pgaptmp_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HGU4558469.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(42392..43597)
                     /gene="csdA"
                     /locus_tag="ACHOSM_RS09660"
                     /old_locus_tag="pgaptmp_001932"
     CDS             complement(42392..43597)
                     /gene="csdA"
                     /locus_tag="ACHOSM_RS09660"
                     /old_locus_tag="pgaptmp_001932"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HGU4558470.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLVRAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFYFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            43789..44016
                     /gene="ygdI"
                     /locus_tag="ACHOSM_RS09665"
                     /old_locus_tag="pgaptmp_001933"
     CDS             43789..44016
                     /gene="ygdI"
                     /locus_tag="ACHOSM_RS09665"
                     /old_locus_tag="pgaptmp_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HGU4558471.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
BASE COUNT        10548 a        10834 c        12022 g        10899 t
ORIGIN      
        1 tatgagaata tctatatcgc aaattgtttt tgaatgacca gaagatatct tgcgaatcta
       61 agttatgcat cttaaatagt cttatttaag atgttcagat ctctttgtgt gcttaactaa
      121 ctgataatac taggatatga cgagagaagt gatagagcgg aataataaca aatgcacatt
      181 atgaaaactg gaatggttca aaattgtagc aattttgaac cattcacaat ggatattagc
      241 taaagaacat taccgatacg cacaggatac ccacgatcag cgtaatcaga tttccgatgg
      301 agcgccaggg tttaagcgcc gggatcagat acgttgacag cgtaggcatg atgaaaagta
      361 tcatggcaat gagcgggccg ctgatcgcat aaatcatcga aatcgcgttc gggttaatgc
      421 aacaaacaat gaaggtaatc agcgatacca acataattga tagtgcgcgg ttaaatgcac
      481 gacttttctt tacaccaacc tgctgtagtg ttgttttgac gagctctgtg gcaccttcaa
      541 taacgccaaa gtacgtaccc aggaatgatt tcgacatcgc aactactgcg acaataatcc
      601 cggaaattga caaccatgct ggggcgttcg gcagcataga aagcgccgat aaaatggtga
      661 ccccttcttc tttagccgct tcaatatacg aaggtggaat agaaagcagg cagctaaaga
      721 caaagaacag tacacttatg cagatgatga gataagcgac tttcataatt tttttgcatt
      781 tatccatagc gtgttcgcca tatttttcac gtctgtctat ggcaaacgta gaaataatgg
      841 gcgtatggct aaaggcgaaa accatcacgg gaatcgatat ccatatctgg tgaagggtat
      901 tctgattgaa ctctacctga gttgttaata gatcaggttg ccaactaccg acaaggtaaa
      961 tggaaagaaa taagaaatag gcaatcaatg ggaataccaa aaatcccatt acccgaatag
     1021 tggcatgacg tcccatcaga aaaatgagat tcaggattaa tacaacaccc agactcacca
     1081 acatacggat gcgaagatca atcaccatat gctttgccag ctgttctgta agtgagttgg
     1141 taattgccac tgcgtatatc aacacgacga caaaaaaggc gatgaagtac agcgtggtaa
     1201 tcagattacc aatcttcttg ccatagtagt gtgttactgc gcccgttatc ccttcacctg
     1261 ctgatgtttt cgatgagagg atgaactggc ataaggcttt atgtggccaa tatgttaaag
     1321 gccaggcgac cagagcagta ataaacagga ccactgcccc tgccgaacct aactgaatag
     1381 gaaggaagag cgtgcctgca ccaacggctg tgccgtagag cgcgaaactc cacagagttt
     1441 cttcttttga ccaaatatta gacattatct gaacgtatca tctacaaatt aaacaaaatg
     1501 tagggcaatt taccataaac taggttaagt atgggagatt aacgccctat ctacctctct
     1561 attggtgggt tagtggttgc aaaccttacg tgccagttat gtctctgact gctactcagg
     1621 catatttcct ttgtgataaa aatcactttt tttaaacaat taagataact gtaattaaca
     1681 atcatttact attgcactgt taatttgtgc gaggttgtga tgaaagacgt tgtgattgtc
     1741 ggggcgttac ggacacctat cggctgcttt cgtggtgcgt tagcgggtca ttccgccgtg
     1801 gaacttggta gcctggtcgt gaaagcgtta atagaacgta ccggcgtgcc tgcatatgcg
     1861 gtggatgaag tgattcttgg ccaggtgttg actgcagggg cagggcagaa tccggcaagg
     1921 caatcggcta ttaaaggtgg tcttcctaat agcgtttctg caatcacgat taatgacgtt
     1981 tgcggttccg ggcttaaggc actgcatctg gctactcagg cgatacagtg tggcgaggct
     2041 gatattgtca tcgccggtgg ccaggaaaac atgagccgcg caccacatgt tctgactgat
     2101 agccgcaccg gtgcacagct tggcaatagc cagttggttg acagtctggt gcatgatggg
     2161 ttgtgggatg ccttcaatga ttatcatatt ggtgtcaccg ccgaaaatct ggctcgcgaa
     2221 tatggcatca gccgtcagtt gcaggatgct tacgcactta gctcgcaaca aaaagcgcga
     2281 gcggcgattg acgccggacg atttaaagat gagatcgtcc cggtaatgac ccaaagtaat
     2341 ggtcagacgt tggttattga taccgatgaa cagccacgca ctgacgccag tgcagaaggc
     2401 ttagcccgtt taaatccttc atttgatagt ctcggttctg tgacagcggg taatgcatca
     2461 tccataaacg atggcgcagc tgcggtaatg atgatgagcg aagccaaagc acgagcgttg
     2521 aatttacccg tgctggcccg catccgcgca tttgccagcg ttggtgtaga tccggcattg
     2581 atgggaattg cgccggtgta tgcgacccgc cgttgcctgg agcgtgtagg ctggcagttg
     2641 gctgatgtcg atcttatcga ggctaatgaa gcgtttgctg cacaggcgct ttcggttggc
     2701 aagatgcttg agtgggatga gcgtcgggtc aatgtcaatg gtggcgcgat cgcactcggt
     2761 cacccgatag gcgcttccgg ttgccgaatc ctggtttctc tggttcatga aatggtgaaa
     2821 cgtaatgccc gcaaagggct ggcaacgctt tgtatcggcg ggggccaggg cgtggcattg
     2881 accattgaac gtgacgagta gctctgcatt tcctccttac cgataaatat ctgccgtcat
     2941 taatctgttg aataatgatg gctggcaagt cgacatacat tttctgggct ccctcatttt
     3001 tccattctca tgacatattc gtgatcgaca ctgcactttt tttaatcctt aaattaaaaa
     3061 tgaaacattg ttttattttt aatttaaaac cctagcccat gggcatatca tccctttata
     3121 gagaaaacga aacagtgttt cactatcgtt ttctattttc acgcttcact gattatcgga
     3181 ggttgatgtg gacgtaagac agagcatcca cagtgcgcac gcaaaaacgc tggataccca
     3241 agggctgcgc aatgaatttt tggttgaaaa ggtatttgtc gccgatgagt acaccatggt
     3301 ttacagccat attgaccgaa ttattgttgg cggcattatg ccgataacta aaacggtttc
     3361 cgttggcggg gaagttggta aacaactcgg cgtaagctat ttccttgaac gtcgcgagtt
     3421 aggtgttatc aatattggcg gtgccggtac gattactgtc gatggccaat gctatgaaat
     3481 cggtcaccgc gacgccctgt atgtgggtaa aggtgcaaaa gaagttgtct ttgccagtat
     3541 tgataccgcc actccggcaa agttttatta caattgcgca cccgcgcata cgacgtatcc
     3601 caccaaaaaa gtcacaccgg acgaagtatc tccagtcacg ttaggcgata acctcaccag
     3661 taaccgtcgc acaattaaca aatattttgt cccggatgtg ctggaaacct gccaattgag
     3721 tatggggctg acggagctgg ctccaggtaa cttgtggaac accatgccgt gtcacactca
     3781 cgagcgccgg atggaagttt atttctattt caatatggat gatgacgcct gcgttttcca
     3841 catgatgggg cagccgcaag aaacgcgtca tattgtaatg cataacgagc aggcggtgat
     3901 ctccccgagc tggtcgatcc attccggcgt cggaaccaaa gcttatacct ttatctgggg
     3961 catggtcggt gaaaaccagg tctttgatga tatggaccat gtggccgtta aagatttgcg
     4021 ctagttgtgg gcataaacga ataaggtatt gttatgattt taagtgcatt ttctctcgaa
     4081 ggtaaagttg cggtcgtcac tggttgtgat actgggctgg gccaggggat ggcgttgggg
     4141 ctggcgcaag cgggctgtga cattgttggc attaacatcg ttgaaccgac tgaaaccatc
     4201 aagcaggtca cggcgctggg gcgtcgtttt ttaagcctga ccgccgatct gcgaaagatt
     4261 gatggcattc cagcactgct ggatcgcgcg gtagcggagt ttggtcatat tgatatcctg
     4321 gtgaataacg ccggattgat tcgccgcgaa gacgctctcg agttcagcga aaaagactgg
     4381 gacgatgtca tgaacctgaa tatcaagagc gtattcttca tgtctcaggc agcggcgaaa
     4441 cactttatcg cgcaaggcaa tggcggcaag attatcaata tcgcgtcaat gctctccttc
     4501 cagggcggga tccgtgtgcc ttcttatacc gcatcaaaaa gcggcgtgat gggtgtgacg
     4561 cgattgatgg cgaacgaatg ggctaaacac aacattaatg ttaatgcgat agccccgggt
     4621 tacatggcga ccaacaatac tcaacaacta cgggcagatg aacaacgtag cgcggaaatc
     4681 ctcgaccgca ttccagctgg ccgttgggga ctgccgagtg acctgatggg gccggtagtg
     4741 ttccttgcct ccagcgcttc agattatgtg aatggttata ccattgccgt ggatggcggt
     4801 tggctggcgc gttaattcat tcttcttact tttatgaccc tgccgcatgg cagggttttt
     4861 tatatctgta gatcatcata atccatatca tggttatgaa ataatccata tcaattatca
     4921 attaatgaac tttatgaatt ttatctgctg taaaattagg tggttaataa taatctcaat
     4981 aattcaactt aatttgaaaa ttggaatatc catcacataa cgacatgtcg tagcaattta
     5041 atccatattt atgctgtttc cgacctgaca cctgtgtgag ttgttcacgt attttttcac
     5101 tatgtcttac tctctgctgg caggaaaaaa tggttactat caatacggaa tctgctttaa
     5161 cgccacgttc tttgcgggat acgcggcgta tgaatatgtt tgtttcggta gctgctgcgg
     5221 tcgcaggatt gttatttggt cttgatatcg gcgtaatcgc cggagcgttg ccgttcatta
     5281 ccgatcactt tgtgctgacc agtcgtttgc aggaatgggt ggttagtagc atgatgctcg
     5341 gtgcagcaat tggtgcgctg tttaatggtt ggctgtcgtt ccgcctgggg cgtaaataca
     5401 gcctgatggc gggggccatc ctgtttgtac tcggttctat agggtccgct tttgcgacca
     5461 gcgtagagat gttaatcgcc gctcgtgtgg tgctgggcat tgctgtcggg atcgcgtctt
     5521 acaccgctcc tctgtatctt tctgaaatgg caagtgaaaa cgttcgcggt aagatgatca
     5581 gtatgtacca gttgatggtc acactcggca tcgtgctggc gtttttatcc gatacagcgt
     5641 tcagttatag cggtaactgg cgcgcaatgt tgggggttct tgctttacca gcagttctgc
     5701 tgattattct ggtggtcttc ctgccaaata gcccgcgctg gctggcggaa aaggggcgtc
     5761 atattgaggc ggaagaagta ttgcgtatgc tgcgcgatac gtcggaaaaa gcgcgagaag
     5821 aactcaacga aattcgtgaa agcctgaagt taaaacaggg cggttgggca ctgtttaaga
     5881 tcaaccgtaa cgtccgtcgt gctgtgtttc tcggtatgtt gttgcaggcg atgcagcagt
     5941 ttaccggtat gaacatcatc atgtactacg cgccgcgtat cttcaaaatg gcgggcttta
     6001 cgaccacaga acaacagatg attgcgactc tggtcgtagg gctgaccttt atgttcgcca
     6061 cctttattgc ggtgtttacg gtagataaag cagggcgtaa accggctctg aaaattggtt
     6121 tcagcgtgat ggcgttagga actctggtgc tgggctattg cctgatgcag tttgataacg
     6181 gtacggcttc cagtggcttg tcctggctct ctgttggcat gacgatgatg tgtattgccg
     6241 gttatgcgat gagcgccgcg ccagtggtgt ggatcctgtg ctctgaaatt cagccgctga
     6301 aatgccgcga tttcggtatt acctgttcga ccaccacgaa ctgggtgtcg aatatgatta
     6361 tcggcgcgac cttcctgacg ctgctcgaca gcattggcgc tgccggtacg ttctggctct
     6421 acactgcgct gaacattgcg tttgtgggca tcactttctg gctcattccg gaaaccaaaa
     6481 atgtcacgct ggaacatatc gaacgcaaac tgatggcagg cgagaagttg agaaatatcg
     6541 gcatctgatt tcacgggccg gatgtgctgt acatccggcc cttttttcgt taatagagat
     6601 cgggcacttg gccgttgagg cgtttgtctc gttccttatt cagccttgtt gcggtaacac
     6661 acatcaggag agaggaatga aaacaattgg tttgctggga ggaatgagct gggaatccac
     6721 cattccttac tatcgtttga taaatgaagg cattaaacag cggcttggtg ggcttcactc
     6781 tgcgcaagtg ctgctacata gcgtcgattt tcatgaaata gaagagtgcc agcgtcgcgg
     6841 ggaatgggat aaaaccgggg acattctggc tgaggcggcg cttggcttac agcgggcggg
     6901 cgcagaaggt attgtgttat gtaccaatac gatgcataaa gtggcggatg ccattgagtc
     6961 acgttgcact ctgcctttct tacacattgc ggatgccacc ggacgtgcaa ttaccggggc
     7021 cggaatgact cgtgtggcgc tgctgggtac gcgttacacc atggaacagg atttttatcg
     7081 cgggcggctg acggaacaat tttccatcaa ttgtcttatt cctgaagcgg atgaacgggc
     7141 gaaaattaat cagattattt ttgaagaact gtgtctgggg caatttaccg aagcgtcacg
     7201 cgcttattat gcgcaagtga ttgctcgcct tgcagaacag ggcgcacagg gcgtcatttt
     7261 tggctgcaca gaaattggtt tactggtgcc agaagagcgc agggttttgc ctgtgtttga
     7321 taccgcggcg atccatgccg aggatgctgt cgcttttatg ctgtcgtagc tgacgacaaa
     7381 atagcgtcaa gagaagtgac cagtttcggt aaccccgctt gtaaatgccc actaaacgcc
     7441 tgaaccagcg ctgatgacgg gcggtgcagg gggcggatca ggctgacggt gaacggaacc
     7501 gcaatactga accgccgcac cactaaaccg cttgccgcat aatctagtgc ggtgagcggg
     7561 ttaaccaccg aaacacctac ccccgcccgt accattgcgc agactgacgc ggcgctgtgg
     7621 gtttctacga tcatgcgtcg tttaacctga tgttcagtaa atagctgatc cagcaactgg
     7681 cgatagctgt cagtacggga aaggctgatg tagttctcac cctgaaaatc atccggcgtt
     7741 aatacctttt ttaccgccag cggatgaccc ggaggtaaca cacacacttc atctaaagag
     7801 agtaattcgg tacgttctgt tcccgcaggc gtatggagcg tttcagtgag tcctaaatca
     7861 tgacgctggg ccgagagcca ctcttcaagt agcggtgatt cctggggcac gatatttaag
     7921 ctgacatcgg gataacgtgc cagaaagggt tgcaggagct gcggtaaaaa agattgcgaa
     7981 aagaccggca ggcaggcaat agacagttct ccctggcgaa actcgcgcag actttctgcg
     8041 gcgctgacaa tgcgatccag tccgtaccag gatcgttgca cttcttcaaa cagacgcagt
     8101 ccttgcacgg taggatgtaa tcgtccacgt acgcgctcaa acaatttcag cccgatcacc
     8161 ttctcaaagc gcgccagttc gcggctgacg gttggctgtg aggtgtgtag caggtgtgct
     8221 gcctcagtca ggcttccggc ggtcattacc gcatgaaaaa tttcaatatg acgtaagtta
     8281 acggcggcca ttagcgctct ctcgcaatcc ggtaatccat atcatttttg catagactcg
     8341 acataaatcg atatttttta ttctttttat gatgtggcgt aatcataaaa aagcactcat
     8401 ctggagtttg ttatgccaca ttcactgttc agcaccgata ccgatctcac cgccgaaaat
     8461 ctgctgcgtt tgcccgctga atttggctgc ccggtgtggg tctacgatgc gcaaattatt
     8521 cgtcggcaga ttgcagcgct gaaacagttt gatgtggtgc gctttgcaca gaaagcctgt
     8581 tccaatattc atattttgcg attaatgcgt gagcagggcg tgaaagtgga ttccgtctcg
     8641 ttaggcgaaa tagagcgtgc gttggcggcg ggttacaatc cgcaaacgca ccccgatgat
     8701 attgttttta cggcagatgt tatcgatcag gcgacgcttg aacgcgtcag tgaattgcaa
     8761 attccggtga atgcgggttc tgttgatatg ctcgaccaac tgggccaggt ttcgccaggg
     8821 catcgggtat ggctgcgcgt taacccgggg tttggtcacg gacatagcca aaaaaccaat
     8881 accggtggcg aaaacagcaa gcacggtatc tggtacaccg atctgcccgc cgcactggac
     8941 gtgatacaac gtcatcatct gcagctggtc ggcattcaca tgcacattgg ttctggcgtt
     9001 gattatgccc atctggaaca ggtgtgtggt gctatggtac gtcaggtcat cgaattcggt
     9061 caggatttac aggctatttc tgcgggcggt gggctttcta ttccttatca acagggtgaa
     9121 gaggcggttg ataccgaaca ttattatggt ctgtggaatg ccgcgcgtga gcaaatcgct
     9181 cgccatttgg gccaccctgt gaaactggaa attgaaccgg gtcgctttct ggtagcgcag
     9241 tctggcgtgt taattactca ggtgcggagc gtcaaacaaa tgggtagccg ccactttgtg
     9301 ctggttgatg ccgggttcaa cgatctgatg cgcccggcaa tgtacggtag ttaccaccat
     9361 atcagtgccc tggcagctga tggtcgttct ctggaacacg cgccaacggt ggaaaccgtc
     9421 gtcgccgggc cattatgtga atcgggcgat gtctttaccc agcaggaagg gggaaatgtt
     9481 gaaacccgcg ccttgccgga agtgaaggcg ggtgattatc tggtactgca tgatacaggg
     9541 gcatatggcg catcaatgtc atccaactac aacagccgtc cgctgttacc agaagttctg
     9601 tttgataatg gtcaggcgcg gttgattcgc cgtcgccaga ccatcgaaga attactggcg
     9661 ctggaattgc tttaactgcg gttagtcgct ggttgcatga tgacttgcct ccagcgacgg
     9721 agttgacact gaatggcgac gtaccagcgt cggactaaag acattagtaa tttccgggag
     9781 agggcgatta tccgccagcg ccaaagccag ttcggcagcc tgggtcgcca tcgtcacgat
     9841 tgggtaacgc acggtggtca ggcgcgggcg cacatagcgt gacaccagca catcatcaaa
     9901 gccaattaac gaaatctcac ccggtacatc aataccatta tcattgagaa cgcccatcgc
     9961 acccgccgcc attgaatcgt tataacaggc taccgcagtg aaatttcttc ctcgtcccaa
    10021 aagctcggtc attgcctgtt cgccgccgct ttcgtctggt tcgccaaatg tcaccagccg
    10081 gtcattggcc gcaataccac tttcagcaag ggcatcgtaa tacccttgca gacgatcttc
    10141 ggcgtcagaa atagagtggt tagagcacag ataaccaatg cgggtatgac cttgctgaat
    10201 taaatgacgc gttgccagcc aggcaccgta acgatcgtca agagcaatac aacggttttc
    10261 aaagccaggc aggatacggt tgatcagcac cataccgggc atttgtttca ttaatgaggc
    10321 taaatcgtca tccgggatca ttttggcatg gacgaccaac gcagcacagc gatggcggat
    10381 cagttgctca atagcctgac gctctttttg ttcgttgtgg taaccgttgc caatcaataa
    10441 gaaattaccg gtgtgataag ccacctgttc gaccgctttc accattgcac cgaaaaacgg
    10501 atcggaaaca tcaccaacga tcagaccgac cgtttcagtg gtctgctgcg ccagcgcacg
    10561 ggcgttggcg ttcgggtgat agctaagaga ctccattgca ctatgcacag ccagccggga
    10621 agcttcgctg gctttaggtg aattattaat gacgcgggaa acggtggcga ctgaaacgcc
    10681 tgccagtcgg gctacatcct ttatggtcgc catgaaaata ccttagtggg taaacgctta
    10741 cattctttca gtgttacgga aaacgcccgt tggttcaagg ggtggcgtgt tttcatcgtg
    10801 ggaatgtgag ccaaacgcaa ataagtaaga caaaaaaaga gattgcaaac ctttggttac
    10861 actttgcgaa acgctgttgc gattgaccgc tggtggcgtt tggctttagg ttgctaaagt
    10921 ggtgatccca gaggtattga taggtgaagt caacttcggg ttgagcacat gaattacacc
    10981 agcctgcgca gatgcgcagg ttttttttgg ccggtcatca atctgtaaca gtaaccgaca
    11041 atttacacac ctcgttgcat ttcccttcat tcctttgcgt tttctcgctg gcgaagagtc
    11101 gtcgtgcaga ccacaatcaa gatcccagag gtattgattg gtgagattat tcggtacgct
    11161 cttcgtaccc tgtctcttgc accaacctgc gcggatgcgc aggttttttt tcgcacctaa
    11221 tttactgtcg ctcgcgttct ttatctcgtg taatctccct ccattcgctt ttactgaatc
    11281 agagcaaagg gagttggaat gctttttagc ttttttcgaa atttgtgccg tgttttgtat
    11341 cgcgttcgcg ttacgggtga cacccaggca ctgaagggcg agcgcgttct aattacgcct
    11401 aatcacgtct cttttattga tggcattttg cttggactgt ttttacctgt gcgtccagtg
    11461 tttgccgttt acacctcaat aagccaacag tggtatatgc gttggctgaa atcatttatc
    11521 gactttgttc ctctcgaccc gacgcaacct atggctatta aacatctggt acgtctggtg
    11581 gaacagggcc gaccagtggt gattttccct gaaggacgca tcaccactac aggctcgctg
    11641 atgaaaatct acgatggcgc gggttttgtc gcggcgaagt ctggtgcaac ggttattcct
    11701 gtgcgtattg aaggggcgga acttacgcac ttcagccgcc tgaaaggtct ggttaaacgt
    11761 cgcttgttcc cgcaaattac tctgcatatt ttgccaccaa cgcaggtgga gatgccggat
    11821 gcgccgcgtg cccgtgaccg tcgcaaaatc gctggcgaaa tgctgcatca aataatgatg
    11881 gaagcgcgaa tggcggtgcg cccgcgtgaa acgctgtacg aatctttact gagtgcaatg
    11941 taccgcttcg gagccgggaa gaaatgtgtc gaagacgtca actttacccc agactcctat
    12001 cgcaaattgc ttacgaaaac gctgtttgtt ggacgcatcc ttgaaaaata cagtgttgaa
    12061 ggcgaacgca tcggcttaat gctgcccaat gcaggcatca gtgcggcagt gatttttggg
    12121 gccatcgccc gtcgccgcat tcccgcaatg atgaactaca ctgccggggt gaaagggctg
    12181 accagtgcta ttacggcggc tgaaatcaaa accatcttca cttcccgcca gtttctcgat
    12241 aaaggcaaac tctggcatct gccggagcaa cttactcagg tgcgctgggt ctatctggaa
    12301 gatttaaaag cagatgtcac cactgccgac aaagtatgga tcttcgcgca tctgctgatg
    12361 ccacgtctgg cacaggtaaa acagcagccg gaagaagagg cgctgatcct ctttacctcc
    12421 ggttctgaag gccatccgaa aggcgtcgtc catagccata aaagcattct ggcgaacgtc
    12481 gagcagatta aaacgattgc cgacttcacc accaacgatc gctttatgtc ggcgttaccg
    12541 ctgtttcact cctttgggct gacggtaggc ctgtttacgc cactgctcac aggtgcagaa
    12601 gtgtttcttt atccaagccc gctgcattac cgtattgtgc cggagttggt gtatgaccgc
    12661 agttgcaccg tgttgttcgg cacctcgact ttcctcggtc actacgcgcg tttcgccaac
    12721 ccgtatgact tctatcgtct acgctatgtg gtggcaggcg cagaaaaatt acaagaaagt
    12781 accaaacagc tttggcagga taaatttggc ctgcgcatcc ttgaaggcta cggcgtgacc
    12841 gaatgcgcgc ctgtcgtttc tatcaacgta ccgatggcgg cgaaacccgg tacggtaggg
    12901 cgtattctac caggaatgga tgcgcgcctg ttgtcggtcc ctggtatcga agagggcgga
    12961 cgcctgcaac tgaaagggcc gaacataatg aacggctatc tgcgggtgga gaagccaggt
    13021 gtactggaag tgcccaccgc cgagaatgtt cgcggcgaaa tggagcgcgg ctggtatgac
    13081 actggcgata ttgtgcgttt tgacgagcag ggctttgtgc agattcaggg ccgcgcaaaa
    13141 cgctttgcca aaattgcagg cgaaatggtg tcgctggaaa tggtggaaca actggcactt
    13201 ggtgtttcgc cagataaagt ccatgccact gcgattaaga gcgatgccag caaaggcgag
    13261 gcactggtgc ttttcaccac agataacgaa ctgacgcgcg ataagttgca acagtatgcc
    13321 cgcgagcacg gcgtgccgga gcttgctgta ccgcgcgata ttcgctatct gaaacagatg
    13381 ccattacttg gcagcggcaa acctgacttt gtcacgttga aaagctgggt agacgaagcg
    13441 gaacaacacg atgagtgagt cagtgcacac taacacttcg ttgtggtcga aggggatgaa
    13501 agcggttatc gtggcgcagt ttctctctgc gtttggcgat aatgccctac tgtttgccac
    13561 tctggcgtta ctgaaagcgc agttctatcc ggagtggagc cagcccatcc tgcaaatggt
    13621 gtttgtaggt gcttacattc tttttgcgcc gtttgtcggg caggtggcgg atagcttcgc
    13681 caaaggccgg gtgatgatgt ttgccaacgg cctgaagctg ctgggcgcag ccagtatctg
    13741 ctttggtatc aatccgtttc tcggctatac gctggtgggt gttggtgctg cagcctattc
    13801 accggcgaaa tacggtattc tcggcgaatt aaccacgggt agtaagttag tgaaagctaa
    13861 cggtttaatg gaagcttcta ccatagcggc gattttgctc ggttccgtag ccggtggtgt
    13921 gctggctgac tggcatgtcc tcgtcgccct ggccgcatgc gcactggcct acggtggtgc
    13981 ggtcgttgcc aatatctaca ttcccaaact ggcggcggcg cgtccggggc agtcctggaa
    14041 tctcatcaac atgacccgca gtttcctgaa tgcctgcacc tcgctatggc gcaatggtga
    14101 aacgcgtttt tcgctggtgg gcaccagttt attctgggga gcgggtgtca cgctgcgttt
    14161 cctgttggtg ctgtgggtac cggtggcgct gggcattacc gataacgcta cgcccaccta
    14221 tctcaacgcg atggtagcga ttggtatcgt ggttggcgca ggtgcggcag cgaagttagt
    14281 tacgctggaa accgtgtcac gctgtatgcc agccgggatt ttgattggcg tggtggtact
    14341 gattttttcc ctgcaacacg agctgctgcc agcctatgcc ttgttgatgc tgattggcgt
    14401 gatggggggc ttttttgtcg ttccgctcaa tgcgttgcta caggagcggg gtaaaaaaag
    14461 cgtcggggcg gggaatgcga ttgcagtaca aaaccttggc gaaaacagcg ccatgttgtt
    14521 gatgctgggc atttactcgc tggcggtaat gataggcatc ccggtcgtgc ccattggcat
    14581 tggcttcggt gcgttgtttg cgctggcaat aacggcgctg tggatctggc agcgccgtca
    14641 ttaatattta acgccggttt taaccggcgt taatcttatg gtgccggata agtataaacc
    14701 tgatgcaccg cttcaatttc agctaatacg tcttcgctta actccagatg caaactttcg
    14761 atgttagttt tcagctgatc catcgtggtt gcgcccagca gagtgctggc aacaaacggt
    14821 tgacggcgta caaacgcgag cgccatctga gcagggtcca ggccatgacg tctggcgata
    14881 tcaacatacg ccgcgacggc tttttgcgtt tgctcaccgc tatagcgggt gaaccgacta
    14941 aagagcgtat tacgtgcgcc agcgggtttt gcaccattga gatatttccc ggtcagcgtg
    15001 ccgaaaccca ggcacgaata ggccagcagt tcgacccctt catactggct gacttctgcc
    15061 agacctactt caaaactgcg gtttaacaga ctgtaagggt tctgaatggt gacaatacgc
    15121 ggcagatcgt gtttgtccgc cagatgcagg tagcgcatta cgccaaatgc agtttcgttc
    15181 gacacgccga tataacgaat ttttcccgcg cgttggtact ctgccagtgc gtccagcgta
    15241 tccagcagcg aaaccgcagg cgcagaatcc gtccagctat aaccgagttt gccgaagcag
    15301 ttggtcgggc gctgcggcca gtgcacctga taaagatcga ggtaatcagt ctgtaggcgc
    15361 ttgaggctgt catgcagcgc ttcgcggata ttcttccgat ccagcgcctg atccgggcgg
    15421 atgcccttgt cattattgcg cgacggtccg ctcactttgg aggcgataat taacttttcg
    15481 cggctgccat gtttcgccag ccagttgccg acgtaggttt cggttaaccc ttgcgtttcg
    15541 gggcgcggag gtactgggta catttcggca acgtcgataa ggttaatgcc ctgagcgacg
    15601 gcatagtcga gttgtgcgtg ggcgtcggct tcgctgttct gttcaccaaa cgtcatcgtg
    15661 ccaagcccca gcgtgctgac ttccagcgaa ctgtggggta tacggtgata ttgcatagcc
    15721 gtttcctttt tataatcacg acataaggaa tataaaaatg gcagagggaa gggaaaaggg
    15781 aaagagaaaa atcttaaggc cagccggatg ctgaccttat ttgttaacgt tcaataattt
    15841 gcgaaacatc atcacggtta atttgcatcg cgttaccttg ctgatcgtga taactcacca
    15901 gcccggtatc atcatcaatt tcaggttttc catcggtcaa aatcatacgg ccatctttgg
    15961 tcgccatgac gtaatcactg gaacacccgg aaacagcaaa cgccagtccg actgcagaaa
    16021 ttattactgc ccattttttc atccggttat cctcatttgg cctacctatg ataatagtct
    16081 gtttataaac cgacgttata tatcttgaaa gcaggaaaat cttaaactgt gagggtaaaa
    16141 ggagtgagta cgccctcccg caggagagcg tacggagtat cagagcggat tctttttgtt
    16201 gcgaatcaag ttgaggcttt caaccgcaat agagaagaac atcgcgaagt agatgtaacc
    16261 tttcggtacg tggatgtcga aactttccag aatcagggta aagcccacca gaatcaggaa
    16321 agagagcgcc agcattttta ccgaaggatg gcgttcgaca aaatcaccaa tcgagcgcgc
    16381 ggcgaacatc atcacgccta cggcaatcac cacggctgcc atcataataa acaggtgatc
    16441 tgacagaccc acagccgtaa tcaccgagtc gaggctaaag ataatatcca gcagcataat
    16501 ctgcacgata gcgccgagga atgatgaaac gcgtgttttc agcccttctt cttcaccttc
    16561 aatggattcg tggatttcct tgctggcttt ccagataagg aacaagccac ccagaagcag
    16621 aatcaaatca cgggcagaaa tttcctgact gaatattgta aaaagcggat ttgtcaggcg
    16681 cgtaacccag gcgattgatg ccagcagcgc cagacgcata accatggctc ccgccaaccc
    16741 cagacggcgc gcatgagcac gttgtgctgt gggaagcttt gccaccacca gagaaaggaa
    16801 aataatattg tcgatcccaa gaacgatctc cagcagcgtc agcgtaccga gcgcaagcca
    16861 ggcgttagga tcggttatcc atgcaaataa catctaaaaa gccctgccaa aaaaatgaag
    16921 cggtaattat atgcccggaa agcggcaggt caaagcgatg gctactggat cagaaaatga
    16981 cgggccagta gtgcactggt gaaattcttc ttcaaataaa agccgcgtgg cagcgtcaga
    17041 atccgttcgc cccgggcacc aatggcttcg gtaagcgctt tcgcattcgc tgctttcggt
    17101 cttatctgta aatattcccc gtgacgagcg gtgatccgct caacctgacc gagaacaatc
    17161 atatccatta attcttccca gtcttcgcgt agctgtcggt cttcctcttc attcgggctc
    17221 cacagcaacg gggatcctac gcgacgctgc gccagcggga tgctgcgctc gccttcaacc
    17281 ggtatccaca gtacgcgttt gagcttgtgg cgcacatggc tggtttccca ggtcaccccg
    17341 ctattacccg ttaacggggc aacacaaacg aatgttgttt ccagcggacg accaagacta
    17401 tccacaggga tagttttaag ttccacgccc agagcagcaa aatcttgctc aggtttactc
    17461 cctgcgctgg cacctagcca gatctccagt aacacgccaa tccagccctt atcgcgtttt
    17521 aaattctctg gcgtaaccag cccggcaagt gccgccagtt ctcccaatgt ataaccagaa
    17581 agttgctgtg cttgcgctaa caactgttct tcagtttcgg gaggagagag cagtgggcga
    17641 ggttgggaca tgtcatgata ccttggttaa aaaacaaaca gcaattatgt gccgaatatt
    17701 cgcagtgtaa ctttttccac atgtgttgtt caattctctg atttatattg ctttttaggc
    17761 tgccggtaat cgagttgcgc aacactgcaa taggttttcc aaatctggtc actgaaaata
    17821 aacaggatct tacaccatgt tatccacaga aagctgggat aactgtgaaa aacctccact
    17881 actgtttcca tttacagcct tgacgtgcga cgaaactcca gtttttagtg gttcagtgct
    17941 taacttagtg gcacattctg tggataaata cggcattggt tgatctttcg ccaacccgta
    18001 aatgagaggt gtgacctggg tcacgaatta ctctttttca ttactcaaat tacatgtaat
    18061 acattgacta ataatgaata ttttaaaatg tctatatcgg gcttattcag aatgctccgg
    18121 gttttcccgt agtaattccg tagttcttca caactctatc cacagaaaag gtgaataaaa
    18181 acggctatcc accccttcct ctgtttataa ctctgcacat aactgtgagt tattcaattg
    18241 ttattagtgt gtaatgccct gcagagagtg gtttaccgtc ttcctggagt atgaaacaat
    18301 cattcgtata taaagcttta ttttgaggta gtccggtgat tgatgacgat ggctaccgcc
    18361 caaacgtagg tatcgtgatt tgtaatcgcc aggggcaggt aatgtgggcc cggcgatttg
    18421 gtcagcactc ctggcaattt ccgcaaggcg gaatcaaccc cggagaatcc gcagagcagg
    18481 cgatgtaccg tgaattgttt gaagaagtag gattaagccg caaagacgtt cgaatccttg
    18541 cttcaacgcg taactggttg cgctacaaat taccgaaacg tttggtgcgt tgggacacga
    18601 agccggtttg tatcggccaa aaacaaaaat ggtttctctt gcagctggta agcggcgatg
    18661 cagaaatcaa tatgcaaacc agcagtacac cagagtttga cggctggcga tgggtaagtt
    18721 actggtatcc ggtcagacag gtggtgtcat ttaaacgtga tgtctaccgt agggtaatga
    18781 aagagttcgc gagtgtggtg atgtcactgc aggaaaatac gccaaaacca caaaacgcat
    18841 ctgcttatcg acgtaaaaga ggttaagtca cgccaattat gctcactcgc ctgcgcgaaa
    18901 tagtcgaaaa ggtagccagc gcaccacgcc tgaatgaggc gttaaatatt ctggttaccg
    18961 acatctgtct tgcgatggat accgaggtct gttcggtcta cctggccgat catgatcgac
    19021 gttgttacta cctgatggcg acccgggggc tgaaaaaacc acgcggtcgc actgtaacgc
    19081 tcgcgtttga tgaagggatc gtcggcctgg ttggcaggct ggcggaaccg ataaaccttg
    19141 cagatgcgca aaagcacccc agcttcaaat acatcccctc cgtaaaagaa gaacgtttcc
    19201 gcgcgttttt aggcgtacca attattcaac gtcgccagtt gcttggtgta ctggtggtac
    19261 agcaacgaga gttgcgccag tatgacgaaa gtgaagaatc cttcttggtg acgcttgcca
    19321 cccagatggc agctattctt tctcagtcgc agttgactgc cttgtttggg caatatcgcc
    19381 agacgcgaat ccgcgcatta ccggcagcac ctggtgtggc gattgccgaa ggctggcagg
    19441 atgccacgtt acctttaatg gaacaggtgt atcaggcatc aacgctggat ccggctctgg
    19501 aacgcgaacg attgaccggg gcgctggaag aagcggcaaa cgagtttcgc cgctacagca
    19561 aacgctttgc cgccggagca caaaaagaaa cggcggctat tttcgatctt tactcgcacc
    19621 tgctttcgga cacccggctg cgtcgcgaat tgtttgccga ggttgataaa ggctcggtgg
    19681 cagagtgggc ggtaaaaacg gtcattgaaa aatttgccga acagtttgcc gcgctaagcg
    19741 ataactatct caaagagcgg gctggcgatt tacgtgcgct gggtcagcga ttgctgtttc
    19801 atcttgatga cgctaatcaa gggccgaacg cctggccgga acgtttcatt ctggtggcag
    19861 atgaactgtc agcgacaact cttgctgagc tgccccagga tcgcttagtc ggtgttgtcg
    19921 tgcgagatgg cgcagccaac tcccatgctg cgatcatggt acgtgcgctg gggatcccta
    19981 ccgtgatggg cgcggatatt cagccttcgg tgctgcatcg taggacgctg atcgttgatg
    20041 gctatcgcgg tgaattgctg gtcgatccgg agccggtact gctgcaagaa tatcagcggc
    20101 taattagtga agagattgag cttagccgtc tggcggaaga tgacgtcaat ttacccgcgc
    20161 agttaaaaag cggtgagcgt ataaaagtca tgctcaatgc tggtttaagc ccggaacatg
    20221 aagaaaaact gggcagccgt attgatggca tcggtcttta tcgcactgaa atcccattca
    20281 tgctgcaaag tggtttcccg tcggaagaag aacaggtggc gcagtatcag gggatgctgc
    20341 aaatgttcaa tgataaaccc gtcaccttgc gtacgctgga tgtcggagca gataagcagc
    20401 tgccttacat gccgatcagc gaagagaatc catgcctggg ttggcgtggg attcgcatta
    20461 cgctcgatca gccggagatc ttcttgatcc aggtgcgggc gatgctgcgt gctaatgccg
    20521 ctacgggcaa cctgaatatt ctgttgccga tggtcacaag cctcgatgaa gttgatgaag
    20581 cacgccgcct gattgaacgt gccggacgtg aagtcgagga gatgatcggt tacgaaattc
    20641 ccaaaccacg tatcggcatc atgctggaag tgccgtcaat ggtatttatg ctgccgcatc
    20701 tggcaaagcg ggtcgatttc atctctgttg gcaccaacga tctgactcaa tacattctgg
    20761 ccgttgatcg caacaatacc cgggtggcga acatttatga cagtcttcat cctgcaatgt
    20821 tacgagctct ggcgatgatc gcccgggaag cggaaataca tggaatcgat ctccgtttgt
    20881 gcggtgaaat ggcgggcgat cccatgtgcg tggcaatcct cattgggctt gggtatcgcc
    20941 atctgtctat gaacggacgt tctgtagcgc gggtaaaata cctgctgcgg cgcattgatt
    21001 atgccgaagc agaaaatctt gcgcagcgta gtctggaagc gcaactggcg accgaagttc
    21061 gccatcaggt tgcagccttt atggagcgtc gcggcatggg cgggctgatt cgcggagggt
    21121 tatagcgcgg atcatataca tatcttttaa cggtatccgg caaccagcca ggtccccttg
    21181 tgctattatt cgcacctttg gagcgcctga aacctgcggc gcgcatttca atcgctgttc
    21241 tctttcagcg aaataacaag aacttgtggt gacagatgac cagtagctat ctgcattttc
    21301 cggagtttga tccggtcatt ttctcaatag gacccgtggc gcttcactgg tacggcctga
    21361 tgtatctggt gggtttcatt tttgcaatgt ggctggcaac acgacgggcg aatcgtccgg
    21421 gcagcggctg gaccaaaaat gaagttgaaa acttactcta tgcgggcttc ctcggcgtct
    21481 tcctcggggg acgtattggt tatgttctgt tctacaattt cccgcagttt atggccgatc
    21541 cgctgtatct gttccgtgtc tgggacggcg gcatgtcttt ccacggcggc ctgattggcg
    21601 ttatcgtggt gatgattatc tttgcccgcc gtactaaacg ttccttcttc caggtttctg
    21661 attttatcgc accactcatt ccgtttggtc ttggtgccgg gcgtctgggc aactttatta
    21721 acggtgaatt gtggggccgc gttgacccga acttcccgtt tgccatgctg ttcccaggct
    21781 cccgtacaga agatattttg ctgctgcaaa ccaacccgca gtggcaatcc attttcgaca
    21841 cttacggtgt gctgccgcgc cacccatcac agctttacga gctgctgctg gaaggtgtgg
    21901 tgctgtttat tatcctcaac ctgtatattc gtaaaccgcg cccaatggga gctgtctctg
    21961 gcttgttcct gattggctac ggcgcgtttc gcatcattgt tgagtttttc cgccagcccg
    22021 acgcgcagtt taccggtgcc tgggtgcagt acatcagcat ggggcaaatt ctttccatcc
    22081 cgatgattgt cgcgggtgtg atcatgatgg tctgggcata tcgtcgcagc ccacagcaac
    22141 acgtttcctg aggaaccatg aaacagtatt tagaactgat gcaaaaagtg ctcgacgaag
    22201 gcacacagaa aaacgaccgt accggaaccg gaacgctttc catttttggt catcagatgc
    22261 gttttaacct gcaggatgga ttcccgctgg tgacaactaa acgttgccac ctgcgttcca
    22321 tcatccacga actgctgtgg tttctgcagg gcgacactaa cattgcttat ctacacgaaa
    22381 acaatgtcac catctgggac gaatgggccg atgaaaacgg cgacctcggg ccagtgtatg
    22441 gtaaacagtg gcgcgcctgg ccaacgccgg atggtcgtca tattgaccag atcactacgg
    22501 tactgaacca gctgaaaaac gacccggatt cgcgccgcat tattgtttca gcgtggaacg
    22561 taggcgaact ggataaaatg gcgctggcac cgtgccatgc gttcttccag ttctatgtgg
    22621 cagacggcaa actctcttgc cagctttatc agcgctcctg tgacgttttc ctcggcctgc
    22681 cgtttaacat tgccagctac gcgttactgg tgcatatgat ggcgcagcag tgtgatctgg
    22741 aagtgggcga ttttgtctgg acggggggcg acacgcacct gtacagcaat catatggatc
    22801 aaactcatct gcaattaagc cgtgaaccgc gtccgctgcc gaagttgatt atcaaacgta
    22861 aacccgaatc catcttcgac taccgtttcg aagattttga gattgaaggc tacgatccgc
    22921 atccgggcat taaagcgccg gtggctatct aattacgaaa catcctgcca gagccgacgc
    22981 tagtgtgcgt cggttttttt taccctccat taaattcttc gagacacctt cccgaaattt
    23041 tgcaacgtcc tgcaacggcg taaatagtcc ggaagatgcg ccgaagaaat agaaactggc
    23101 cctcgtcttt ttttctcctc gctccatact gccggcatga aaacacaacg tggttatacg
    23161 ctgattgaaa cgctggtcgc gatgctgatt ctggtcatgc taagcgcaag tggactctat
    23221 ggctggcaat actggcagca gtcgcaacgg ctttggcaaa ccgccagcca ggcgcgggac
    23281 tatttgctct atttacgtga agatgccaac tggcataacc gcgaccacag tatcagtgtt
    23341 atcagagagg ggacgttatg gtgccttgtg agttccgttg ctggggcgaa tacctgtcat
    23401 ggcagttcac cattggtctt tgtgccacgc tggcccgaag tcgaaatgag cgacctgaca
    23461 ccttcgcttg ctttctttgg cctgcgcaat accgcatggg ccgggcatat tcgcttcaaa
    23521 aactcaacgg gcgagtggtg gctggtggtt tcgccgtggg gaagactccg gctttgtcag
    23581 caaggagaaa cagaaggatg cctgtaaaag agcaaggttt ttctctgctg gaagtgttga
    23641 ttgctatggc gatcagtagc gtattgttgc tgggggctgc acgctttctg cctgcgttac
    23701 agcgtgaaag tttaacgagc acccgtaagc tggcgctgga agatgaaatc tggctgcggg
    23761 tatttaccgt cgcgaagcat ctccagaggg cgggttattg tcatggcagc tgtaccggcg
    23821 aagggctgga aattgtcgga cagggtgact gtatcattgt gcagtgggat gcgaacagta
    23881 acggtatctg ggatcgcgaa ccggtaaaag agtctgacca gattggattt cgtctgaagg
    23941 agcatgtgct ggaaacgcta cgcggtgcga catcctgtga aggtaagggc tgggataaag
    24001 tcactaatcc ggatgccatc attatcgaca cttttcaggt cgtacgtcag gatgtcagcg
    24061 gcttctcgcc ggtgttgacg gttaatatgc gtgctgccag taagtctgaa ccgcaaaccg
    24121 tggtggatgc cagctatagc gtgacaggat tcaacctgtg aaccgcgaaa aaggtgtttc
    24181 gtcactggct ctggtcctga tgctgctggt tttgggtagc ttgctattgc aaggaatgag
    24241 tcagcaggat cgcagttttg cctctcgcgt gagcatggaa agtcagtcat tgcgccgcca
    24301 ggccatcgtt cagtcggcgc tggcgtgggg gaaaatgcac tcctggcaga cgcagcccgc
    24361 agttcagtgc tcgcagtacg ctggaaccga tgcccgggtt tgtttgcgtc tactggcaga
    24421 taatgaagcc ttattggttg ccggttatga aggcgtttcg ttgtggcgaa caggcgaagt
    24481 cattgatgga aacattgttt tttcgccacg cggctggagc gatttttgtc cgctgaaaga
    24541 gagggcgtta tgtcagcttc cctgaagaat caacaaggct ttagcctgcc ggaggtaatg
    24601 gttgcgatgg tgctgatggt gatgattgtc actgcgttat cgggtatcca gcgaacatta
    24661 atgaacagtc tcgccagcag aaaccagtac caacagctct ggcggcatgg ttggcagcaa
    24721 acgcaactgc gcgcgatttc gccacctgcc aactggcagg tcaaccgaat gcagacatcg
    24781 caggcgggat gtgtcagcat cagcgttacg ctagtttcac ccgggggcag agaaggcgag
    24841 atgacccgcc tgcattgccc gaatcgtcag tagtcaggag ccgctatgtt aagggtctac
    24901 cattccaatc gtctggacgt gctggaagcg ttgatggagt ttattgtcga acgcgaacgg
    24961 ctggacgatc ctttcgaacc agagatgatt ctggtgcaaa gtaccggtat ggcgcagtgg
    25021 ctgcaaatga ccctgtcgca aaagtttggt attgcggcaa acattgattt tccgctgcca
    25081 gcgagcttta tctgggatat gttcgtccgg gtgttaccgg aaatccccaa agagagcgcc
    25141 tttaacaaac agagcatgag ctggaaactg atgactctgc tgccgcaatt gctggagcgc
    25201 gaagacttta ccctgttgcg gcattatctg actgacgata gcgacaagcg aaaactgttc
    25261 cagctttcct caaaagcggc ggacctgttt gaccagtatc tggtctatcg tccggactgg
    25321 ctggcacagt gggaaacagg acatttggtt gaagggctgg gagaagcaca ggcctggcaa
    25381 gccccgttgt ggaaggcgct ggtggaatat acccatcaac tcgggcaacc gcgctggcac
    25441 cgtgccaatc tctatcagcg ctttatcgaa acgctggagt ccgcgacgac ctgcccgccg
    25501 gggttacctt cgcgcgtctt tatatgcggt atttccgcgt taccgcctgt ttatctccag
    25561 gcgctacagg cgctgggtaa acatattgaa atccatctcc tgtttaccaa cccctgccgt
    25621 tattactggg gcgatattaa agatcctgct tatctggcga aactgctgac ccgtcagcgt
    25681 cgacacagtt ttgaagatcg cgaattgccg ctatttcgtg atagcgaaaa tgccgggcag
    25741 ctctttaaca gcgatggtga acaggatgtc ggcaacccgc tgctggcttc atggggtaag
    25801 cttgggcgcg actacattta tctcctttct gacctggaga gcagccagga gctggacgcc
    25861 tttgtcgatg tcacgccaga taacctgctg cataacattc agtctgacat tctggaactg
    25921 gaaaaccgtg ccgttgctgg tgtgaacatc gaagagtttt cccgtagcga taacaaacgc
    25981 ccgcttgatc cactggatag cagtatcacc ttccacgttt gccatagccc gcagcgtgaa
    26041 gttgaagttt tgcacgatcg cctgctggcg atgctggaag aagacccgac acttactccg
    26101 cgcgacatca tcgtgatggt ggctgatatc gacagctaca gtccgtttat tcaggctgtg
    26161 tttggtagcg cacctgcgga tcgttacctg ccttacgcca tttccgaccg tcgggcgcgg
    26221 cagtcgcatc cggtactgga agcgtttatc agcctgttat cgttgccaga cagccgcttt
    26281 gtgtcggaag acgtgctggc attactggat gtgccggtgc tggcggcgcg gtttgacatc
    26341 accgaagaag ggctgcgtta tttacgccag tgggtcaacg aatccggcat tcgttggggg
    26401 atagatgacg acaacgttcg cgagctggaa ctccccgcca ccggacaaca cacctggcga
    26461 tttggcctga cgcgtatgtt gctgggctac gcgatggaga gcgcgcaggg cgagtggcaa
    26521 tcggttctac cttatgatga atcgagcggc ttaattgcag aactggtggg gcatctggct
    26581 tcactgctaa tgcagctaaa catctggcgt cgcgggctgg cacaggagcg tccgctagaa
    26641 gagtggttgc cggtttgtcg cgatatgctc aacgccttct tcctgccgga tgcggaaacc
    26701 gaagcggcga tgacgctgat cgaacaacaa tggcaggcga ttatcgccga aggtttaggt
    26761 gcgcagtatg gcgacgcggt gccgctgtca ctattgcgtg atgaactggc acagcgtctg
    26821 gatcaagaac gtatcagcca gcgttttctc gccggaccgg ttaacatttg tactctgatg
    26881 ccaatgcgtt caattccgtt caaagtggtt tgcctgctgg gaatgaacga cggcgtttat
    26941 ccacgtcagc ttgcgccatt gggctttgac ctgatgagcc agaaaccgaa gcgtggcgac
    27001 cgtagccgtc gcgatgacga ccgctacctg ttcctggaag cgttaatttc cgcgcagcaa
    27061 aaactctata tcagctatat cgggctttcc attcaggata acagtgaacg tttcccgtcg
    27121 gtactggtgc aggaactgat cgactacatc gggcaaagtc attatctacc gggcgatgaa
    27181 gcgctcaact gtgatgaaag cgaggcaagg gtaaaagcgc atcttacttg cctccatacc
    27241 cggatgccgt ttgatccaca aaactaccag ccaggcgaac gacaaagcta tgctcgtgaa
    27301 tggctacctg cggccagcca ggctggtaaa gcacattctg aatttgttca gccgctgccg
    27361 tttaccttac cggaaaccgt gccgctggaa acgctacaac gattctgggc acatccggtg
    27421 cgggcatttt tccagatgcg tttgcaggtg aacttccgta ccgaagacag cgaaatcccc
    27481 gacaccgagc catttattct ggaagggctt agccgttatc aaatcaatca gcagttattg
    27541 aatgcactgg ttgagcagga tgatgccgaa cgcttgttcc gtcgcttccg ggcggcaggg
    27601 gatttaccgt atggcgcttt tggtgaaatt ttctgggaaa ctcagtgcca ggagatgcag
    27661 cagcttgccg acagagtcat tgcctgtcgc cagccggggc agagtatgga aattgatctc
    27721 gcctgcaacg gtgtgcagat aactggctgg ttgccgcagg tgcagccgga tggcctgttg
    27781 cgctggcgtc cctctttatt aagtgtggcg cagggaatgc aactttggct ggaacacctt
    27841 gtctactgtg ccagcggtgg taatggtgaa agtcgccttt ttctacgcaa agacggcgag
    27901 tggcgttttc cgccgcttgc agccgaacag gctttgcatt acctctcaca actgattgag
    27961 gggtatcgtg aagggatgtc cgcgccattg ctggtgttac ctgaaagtgg cggcgcgtgg
    28021 ctaaaaacct gttatgacgc gcaaaacgat gccatgctgg atgacgattc cacgttgcaa
    28081 aaagcccgta cgaaattcct tcaggcttac gaaggcaaca tgatgatgcg tggcgaaggt
    28141 gatgatatct ggtatcaaag gctctggcgg caattaacac cagagacaat ggaggccatc
    28201 gttgaacagt cgcaacgttt cctgttaccg ctgtttcgct ttaatcagtc atgagtgctg
    28261 tataaaaatt gcgcaatcta tccgcttact ttatgatgcg caccagtcac ggactgatgg
    28321 ttatataaac ataggctgac tcgtgcagca caagattaaa ttctggcaga tgattttcgt
    28381 taacctgttg aatctggcca gaaaataaag ttgattatga ggtccgtgaa tgccccgcag
    28441 cacctggttc aaagcattat tgttgtttgt tgccctttgg gtacccttaa gtcaggcaga
    28501 aacgggatgg cagccgattc aggaaaccat ccgtaaaagt gataaagata accgccagta
    28561 tcaggctata cgtctggata acggtatggt ggtcttgctg gtttctgatc cgcaggcagt
    28621 taaatcgcta tcggcgctgg tggtgcccgt tgggtcgctg gaagatcccg aggcgtacca
    28681 ggggctggca cattaccttg aacatatgag tctgatgggg tcgaaaaagt acccgcaggc
    28741 tgacagtctg gccgaatatc tcaaaatgca cggtggcagc cacaatgcca gcactgcgcc
    28801 gtatcgcacg gctttctatc tggaagttga gaacgacgcc ttgcccggtg cggtagaccg
    28861 cctggccgat gctattgcag aacccttgct cgacaagaaa tacgccgaac gtgagcgtaa
    28921 tgcagtgaac gctgaattaa ccatggcgcg tacgcgtgac gggatgcgca tggcacaggt
    28981 cagcgcagaa accattaacc cggcacaccc cggttcaaag ttttctggtg gtaacctcga
    29041 aactttaagc gataaacctg gtaatccggt gcagcaggcg ctgaaagatt tccacgagaa
    29101 gtactattcc gccaatctga tgaaggcggt tatttacagc aataaaccgc tgccggagtt
    29161 ggcaaaaatg gcggcggaca cctttggtcg cgtgccgaac aaagagagca aaaaaccgga
    29221 aatcaccgtg ccggtagtca ccgacgcgca aaagggcatt atcattcatt acgtcccggc
    29281 actgccgcgt aaagttctgc gcgttgagtt tcgcatcgat aacaactcag cgaagttccg
    29341 tagtaaaacg gatgaattga ttacctatct gattggtaat cgcagcccag gtacactttc
    29401 cgactggctg caaaagcagg gattagttga gggcattagc gccaactccg atcctatcgt
    29461 caacggcaac agcggcgtat tagcgatctc tgcgtcttta accgataaag gcctggctaa
    29521 tcgcgatcag gttgtggcgg caatttttag ctatctcaat ctgttacgtg aaaaaggcat
    29581 tgataaacaa tacttcgatg aactggcgaa tgtgctggat atcgacttcc gttatccgtc
    29641 gatcacccgt gatatggatt acgtcgaatg gctggcagat accatgattc gcgttcctgt
    29701 tgagcatacg ctggatgcag tcaatattgc cgatcggtac gatgctaaag cagtaaagga
    29761 acgtctggcg atgatgacgc cgcagaatgc gcgtatctgg tatatcagct cgaaagagcc
    29821 gcacaataaa acggcttatt ttgtcgatgc gccgtatcag gtcgataaaa tcagcgaaca
    29881 aactttcgct gactggcaga aaaaagctgc cgacattgcg ctctctttgc cagagcttaa
    29941 cccttatatt cctgacgatt tctcgctgat taagtcagag aagaaatacg accatccaga
    30001 gctgattgtt gatgagtcga atctgcgagt agtgtatgcg ccaagccgtt attttgccag
    30061 cgagcccaaa gccgatgtca gcctgatttt gcgtaatccg aaagccatgg acagcgcccg
    30121 caatcaggtg atgtttgcgc tcaatgatta tctcgcaggg ctggcgcttg atcagttaag
    30181 caaccaggcg tcggttggtg gcataagttt ttccaccaac gctaacaacg gccttatggt
    30241 taatgctaat ggttacaccc agcgtctgcc gcagctgttc caggcattgc tcgagggcta
    30301 ctttaactat accgctacgg aagatcagct tgagcaggcg aagtcctggt ataaccagat
    30361 gatggattcc gcagaaaagg gcaaagcgtt cgagcaggcg attatgcccg cgcagatgct
    30421 ctcgcaagtg ccgtacttct cgcgagatga acggcgcaaa attttgccct ccattacgtt
    30481 gaaagaggtg ctggcctatc gcgacgcctt aaaatcaggg gctcgaccag agtttatggt
    30541 tattggcaac atgaccgaag ccctggcaac aacgctggca cgcgatgtgc aaaaacagtt
    30601 gggcgctgat ggttcggagt ggtgtcgtaa caaagatgtc gtggtcgata aaaaacaatc
    30661 cgtcatcttt gaaaaagccg gtaacagcac cgactccgca ctggcagcgg tatttgtacc
    30721 gactggctac gatgaataca ccagctcagc ctatagctca ctgttggggc agatcgtaca
    30781 gccgtggttc tacaatcagt tgcgtaccga agaacagttg ggctatgccg tgtttgcgtt
    30841 tccaatgagc gtggggcgtc agtggggcat gggcttcctt ttgcaaagca atgataaaca
    30901 gccttcattc ttgtgggagc gttacaaggc gtttttccca accgcagagg caaaattgcg
    30961 ggcgatgaag ccagaagagt ttgcgcaaat ccagcaggcg gtaattaccc agatgctgca
    31021 ggcaccgcaa acgctcggcg aagaagcatc gaagttaagt aaagatttcg atcgcggcaa
    31081 tatgcgcttc gattcgcgtg ataaaatcgt ggcccagata aaactgctga cgccgcaaaa
    31141 acttgctgat ttcttccatc aggcggtggt cgagccgcaa ggcatggcta ttctgtcgca
    31201 gatttccggc agccagaacg ggaaagccga atatgtacac cctgaaggct ggaaagtgtg
    31261 ggagaacgtc agcgcgttgc agcaaacaat gcccctgatg agtgaaaaga atgagtgatg
    31321 tcgccgagac actagatcct ttgcgcttgc ccttacaggg tgagcgcctg attgaagcct
    31381 ctgccggcac aggcaaaacc tttacgattg cggcgctcta tttgcgcctg ttacttggac
    31441 taggcggttc cgccgccttt ccccgcccgc tgaccgttga agaactgctg gtggtcacct
    31501 ttaccgaggc tgccacggca gaattgcgtg gtcgtatccg tagcaatatc cacgagttgc
    31561 gcatcgcctg tctgcgtgaa accaccgaca atccactgta cgaacgcctg ctggaagaga
    31621 tcgacgataa agcgcaagcc gcgcagtggt tgttgttagc cgaacggcag atggatgaag
    31681 cggcagtctt tactattcac ggtttttgcc agcgcatgct caacctgaat gcctttgaat
    31741 ccggtatgct gtttgagcag cagctgattg aagatgagtc tctgctacgc taccaggcct
    31801 gcgccgattt ctggcgtcgc cactgctacc cgctgccgcg tgaaattgcc ctggtggtct
    31861 ttgaaacctg gaaagggccg caggcgttgc tgcgcgatat taatcgttat ctgcaaggcg
    31921 aagcaccggt tatcaaagca ccgccgcccg atgatgaaac gctggcttcc cgccacgcgc
    31981 aaattgtggc gcgtattgat acggtaaaac agcagtggcg cgacgcagtg ggtgaactgg
    32041 atgcgctgat cgaatcttct ggtattgatc gacgcaagtt taaccgtagc aatcaggcta
    32101 aatggatcga gaagatcagc gcctgggcag aagaagagac caacagttat cagttaccgg
    32161 agtcgctgga aaaattctct cagcgtttct tagaagatcg cacgaaagcc gggggggaaa
    32221 ccccgcgaca tccactgttt gaggcgatcg atcaactgct tgcagaacca ttgtcgatcc
    32281 gtgatctggt gataacccgc gcattggctg agatccgcga aacagtagcg cgtgaaaaac
    32341 gccgccgtgg cgaattgggt tttgatgaca tgttaagtcg gctcgattcc gcgctgcgta
    32401 gcgaaagcgg cgaggtgttg gcagcggcga tccgtacgcg attcccggtg gcaatgattg
    32461 atgaatttca ggataccgac ccccagcagt accgaatttt tcgccgtatc tggcaccatc
    32521 agccggaaac cgcattgttg ctgattggcg acccgaaaca ggccatatat gcattccggg
    32581 gtgcggatat cttcacttat atgaaggcgc gtagcgaagt tcacgcccac tacactttag
    32641 acaccaactg gcgttccgca ccaggaatgg tgaacagtgt gaacaagctt ttcagccaga
    32701 ctgatgacgc gttcatgttt cgcgaaatac cgtttattcc agtgaaatca gccgggaaaa
    32761 atcaggcgtt acgttttgtg tttaaaggtg aaacgcagcc tgcgatgaaa atgtggctga
    32821 tggaaggcga aagctgcggc gttggcgact atcaaagtac catggcgcag gtatgtgctg
    32881 cgcaaatccg cgactggcta caagccggac agcggggcga agcgttgctg atgaacggcg
    32941 acgacgcgcg tccggtgcgt gcttcggaca tcagtgtgct ggtgcgcagc cgccaggagg
    33001 ccgcccaggt gcgcgacgcc ttaacgttgc tggaaatccc ttccgtttac ctttcgaacc
    33061 gcgacagtgt ttttgaaact ctggaagcgc aggaaatgct ttggttgttg caggcggtga
    33121 tgacgcccga acgtgagaac accctgcgta gtgcgctggc aacgtcaatg atggggctga
    33181 acgcgctgga tatcgaaacg ctgaacaatg acgaacatgc gtgggatgcg gtagtcgaag
    33241 agttcgatgg ttatcggcaa atctggcgca aacgtggcgt tatgccgatg ctgcgggcgc
    33301 tgatgtcggc gcgtaacatt gcagaaaact tgctggcaac ggcaggcggt gagcggcgtc
    33361 ttaccgatat cttgcatatc agcgaactgc ttcaagaagc cggaacgcag ctggaaagtg
    33421 aacatgcgct ggtacgctgg ttatcgcaac atatcctcga gccagacagt aatgcctcca
    33481 gccaacaaat gcgcctcgaa agtgataaac atctggtgca gattgtcacg atccataaat
    33541 cgaaagggct ggaatatccg ctggtctggt tgccatttat taccaatttc cgcgtccagg
    33601 atcaggcgtt ttatcacgat cgccactcgt ttgaggcagt tttggatctt aatgctgcgc
    33661 cagaaagcgt cgacctcgcg gaggtcgaac gtctggcgga agatctacgt ttgctttacg
    33721 tggcgctgac gcgttcggtt tggcattgca gtctcggcgt tgcaccgctg gtgtgccgtc
    33781 gtggcgataa aaaaggtgac accgacgtcc accaaagtgc gctcgggcgt ttgctgcaaa
    33841 aaggggaacc gcaagatgcg gcagggcttc gcacctgtat tgaagcgtta tgcgatgatg
    33901 atattgcctg gcaaacggca caaactggtg ataaccagcc ctggcaggtt aatgatgctt
    33961 taactgcaga actgaatgcg aggacactac aacgattgcc cggcgataac tggcgtgtca
    34021 ccagctactc cggcttgcag cagcgtggtc acggtatcgc tcaggatctg atgccacggc
    34081 tggatgttga tgccgcaggc gtggtcagcg tcgttgaaga accgacgtta acaccgcatc
    34141 agttcccgcg cggtgcgtca ccggggacat tcttgcacag tttgtttgaa gacctcgatt
    34201 ttacccagcc ggttgacccg aactgggtac aggaaaaact ggaactcggc ggctttgaat
    34261 cgcagtggga accggtgttg accgagtgga tcacggctgt cctccaggca cctctcaatg
    34321 aaacgggtgt tagcctgagt cagctttccg accgcgataa acaggtggag atggagtttt
    34381 acctgccgat tagcgaaccg ctcatcgcca gccagcttga tgcactaatt cgccagtttg
    34441 acccgctatc cgctggctgc ccgccgctgg agttcatgca ggtacgcggc atgttaaaag
    34501 gctttatcga cctggtattc cgccacgaag ggcgttatta cctgctcgac tataaatcca
    34561 actggctggg tgaagacagt tcggcttaca cccaacaggc tatggcagca gcgatgcagg
    34621 cacaccgcta tgatctgcaa tatcagcttt ataccctggc gctgcatcgt tatctgcgcc
    34681 atcgcattgc tgattacgac tatgatcgtc actttggcgg cgttatctat ctgttcctgc
    34741 gtggcgttga taaagaacat ccgcaacaag ggatttacac aacccgaccc aacgccgggt
    34801 tgattgccct gatggatgag atgtttgccg gtatgaccct ggaggaggcg taatgaaatt
    34861 gcaaaagcaa ttactggaag ctgtggagca caaacagcta cgcccgctgg atgtgcaatt
    34921 tgccctgacc gtggcgggag atgaacatcc tgccgtcacc ctcgcggcgg cactgttaag
    34981 tcatgatgcc ggagagggac acgtctgttt gccgctttca cgactggaaa ataacgaggc
    35041 gtcgcatccg ctgttggcga cctgtgtcag tgaaatcggt gagctacaaa attgggaaga
    35101 atgcttgctg gcttctcaag cggtcagcag gggagatgaa cccacgccga tggtcctctg
    35161 tggcgatcgt ctttatttga atcgcatgtg gtgtaacgag cggacggtag cgcgcttctt
    35221 caatgaggtg aatcatgcca ttgaggttga tgaagctcta ctggcgcaaa ccctggacaa
    35281 actttttcca gtaagcgatg aaattaactg gcaaaaagtt gcagcggcag tggcgctgac
    35341 gcggcggatc tcggtgattt ccggcggccc aggcaccggt aaaacgacca ccgtggcgaa
    35401 gttgctggca gcgttaattc aaatggccga cggcgaacgc tgccgtatcc gtctggctgc
    35461 accaacgggt aaagctgccg cgcgcttaac cgaatctctc ggcaaggctt tgcgacagtt
    35521 accgctgacc gatgaacaaa agaaacgcat tccggaagat gccagcactt tgcaccgatt
    35581 gctgggcgcg cagccgggta gccagcgttt acgtcatcat gccggtaacc cgctgcatct
    35641 tgatgtgctg gtggtagatg aagcgtcaat gatcgatctg cctatgatgt cgagactgat
    35701 cgacgccttg ccagatcatg cgcgagtgat ctttctcggc gatcgtgatc aactggcctc
    35761 ggttgaggct ggggctgtgc tgggcgatat ctgcgcttat gccaacgcgg gctttaccgc
    35821 cgagcgtgcc aggcagctaa gccgcctgac ggggactcac gttccgacag gaaccggcac
    35881 agaagcggca tctttgcgcg acagtctctg cctgctgcaa aaaagctatc gtttcggcag
    35941 cgattctggc attggtcagt tagctgcggc gatcaaccgt ggtgataaaa cggcagtgaa
    36001 aaccgttttt cagcaggatt ttactgatat cgaaaaacga cttttacaga gcggcgaaga
    36061 ttatattgcg atgcttgagg aagctcttgc gggttatgga cgttatctgg atctgctgca
    36121 agcgcgtgcc gagccggatt taatcattca ggcgttcaat gagtaccaac ttttgtgcgc
    36181 cctgcgggaa gggccgtttg gcgtggctgg actgaatgag cgaattgagc agtttatgca
    36241 acagaagcgc aaaattcatc gtcatccgca ctctcgttgg tacgaaggtc gaccggtgat
    36301 gattgcccgt aatgacagcg cgcttgggtt gtttaatggc gatatcggta ttgcgctgga
    36361 tcgcgggcag gggacgcgcg tctggtttgc gatgccggac ggcaatatta agtctgtgca
    36421 accgagtcgc ttgccagagc acgaaactac gtgggcgatg acggtacata aatcgcaggg
    36481 atcggagttc gaccatgcgg cgttgatttt accgagccaa cgcacgccgg tagtaacgcg
    36541 agagctggta tacaccgcgg tgacccgcgc gcgtcgccgt ctgtcgctgt atgccgatga
    36601 gcgtatatta agtgcggcaa tcgccactcg tactgagcgg cgcagtggtc tggcggcgtt
    36661 gtttagttcg cgggaataaa cgtaattgcc ggatgcgacg cacgagtgtt acgcatgtcg
    36721 catccgacaa ttttcatcgc ttaccctaaa tccgccatca acactttgga tttacgctgg
    36781 tagttgtaca actgcttttt gctctcgggc agtaaatcaa tatccaccgg ggtaaatcca
    36841 cgttcctgga accagtgaat actgcgcgtg gtcagcacaa acaatttgct taagccgctc
    36901 tgcttcgcct gagcggtaat gcgttccagc agaacctcgc cccgtgatga gctgcggtaa
    36961 tccgggtgaa ctgccacaca ggccatttcc ccaatcttct cttccgggaa cggatagagc
    37021 gcggcgcagg caatagtcgt gttatcgcgc tgaataatgg tgaatttgtc gatttccatc
    37081 tccagctgct cgcgagaacg gcgcaccaga ataccttgct gctccagcgg gcgaatcaac
    37141 tccagaatac cgccaatatc gttgattgtt gcgcgacgaa tctgctcagc gctttccatc
    37201 acaatctgcg taccgatacc gtcgcgtgag aacaactctt gcaacagcgc gccatcttcc
    37261 tgataactga ttaaatgaca gcgacgcacg ccgctgcggc aggctttcac tgcgccacgc
    37321 aaaaagcgca ccgtaccgga gttgtaatcg cctttctctt cctgggcttc tacccgcgct
    37381 tgcgcttcgt tagggaaaag ttctgagaca atatcaccgt tgtcattagt gacgccctgt
    37441 gaagagcaaa aaccaatcat cttctcagct ttcagtttga tggccagttg agtggcaatc
    37501 tcttccgagg tcagattaaa gctctcgcca gtgactgaaa cagcgaccgg ccccattagc
    37561 actattgcac cgctgtccag ttgacgatgg atcgcgtctt catcaatccg ccggatacgc
    37621 ccgctatggc agtaatccac gccgtcatcg acgcccagcg gctgggcaat aataaaattg
    37681 ccactgacga cgttgatatg cgcgccctgc agcggcgtgt tattgagact catcgacagg
    37741 cgagcagtaa tatccagttg caatgttccc gcagcctgct tcaccagttc cagtgttttg
    37801 gcgtcggtca cacgtatatt cttgtgatac agcggttcgt ggtgatgcgc agccagattt
    37861 gcgtcgatct gcggacgtgc gccatagacc accaccagac ggatgccgag gctgtgcaat
    37921 aacccgatat cattaacgat actggagaaa ttctcatgct caatggcttc accgccgagc
    37981 atgatgacaa acgtttttcc ccggtgggta ttgatatagg gaaccgaatg gcggaatccc
    38041 tcgaccaact cggttttacg ttcctttacc acggcacacc tctttgcatg attattcgaa
    38101 attagtgtat ttttattctg ttttttcgca gggtgcaagt gtaaatttta tgcgagagcg
    38161 acttttttat cagtaatgcc gtgaatataa aaagaatgtt taccgtttat tagatgacag
    38221 attatgcgtc tttcgctaaa gtttccggtc aaattagtcg tttacttgtt acacagctta
    38281 gatttctttt gctcgggaga ggcatgtcag gatccaacac tgcaatcagc cgtcgtcgtt
    38341 tactgcaagg cgcgggtgcc atgtggctat tgagcgtaag tcaggtcagt ctggctgcgg
    38401 tcagccaggt cgtggcggtg cgcgtctggc ctgcgtccag ctacacccgt gtgacggtag
    38461 aatcaaatcg tcagctgaaa tataagcagt tcgcgttgag taacccggaa cgcgtggtgg
    38521 tggatatcga agatgtaaac ctgaactcgg tactcaaggg gatggcggca caaattcgtg
    38581 cagacgaccc gttcatcaag tcggcgcgcg tcgggcaatt tgacccgcaa accgtacgga
    38641 tggtttttga attaaagcaa aacgtaaaac cgcagctgtt tgcccttgcg ccggtcgccg
    38701 ggtttaaaga gcgtctggtg atggacctct atccggccaa tgcacaggat atgcaggacc
    38761 cgctgctggc gctgctggag gattacaaca aaggcgacct cgaaaagcag gtgccgccag
    38821 cgcaaagtgg tccacaacca ggtaaagcag ggcgcgatcg tccgattgtc attatgcttg
    38881 accctggcca cggtggcgaa gactccggtg cggtggggaa atacaaaacg cgcgaaaaag
    38941 acgtggtatt gcaaatagct cgccgtttgc gctctctgat cgagaaagag ggcaatatga
    39001 aggtgtacat gacgcgcaat gaagacatct tcattccgtt gcaagtgcgc gtagcaaaag
    39061 cccagaaaca gcgtgctgac ctgtttgtct ctatccatgc cgacgccttt accagtcgtc
    39121 agccgagcgg ttcctctgtg tttgcgctct caaccaaagg tgcaaccagt actgcggcaa
    39181 aatatctggc acaaacccag aacgcctcgg acttgattgg tggcgtgagc aaaagcggtg
    39241 accgctatgt cgaccacacc atgttcgata tggtgcagtc gctgaccatt gccgacagcc
    39301 tgaagtttgg taaagcggta ctgaataagc tcggtaaaat caacaagctg cataaaaatc
    39361 aagttgaaca ggccgggttt gccgtactaa aggcaccaga tattccctcc attctggtcg
    39421 aaacggcgtt tatcagtaac gttgaggaag agcgtaaact gaaaacggcg actttccagc
    39481 aggaagttgc ggagtctatt cttgcgggga ttaaagcgta ttttgccgat ggggcaacgc
    39541 tggcgagaag gggatgataa aaaggcgctg aatggcgctt tttttatggg agaccagaaa
    39601 caaaaaaaac acccgttagg gtgttcaata attggttgcg ggggccggat ttgaaccgac
    39661 gaccttcggg ttatgagccc gacgagctac caggctgctc caccccgcgt ccgtggatgc
    39721 gcactatact ccgctcgcgt tttcatgcaa cctttttttt accccgatca cggatttggc
    39781 atgattttga acaaaaacaa atcactaaag cctatttttt gtgcaaagtt tgcgtcaggg
    39841 agaggctgct catttcatta aacgcggcgg tttgttatct tcgttgcgcc ttatttttta
    39901 acctgaagaa gagaacaatg aaaggacgtt gggtaaagta ccttcttatg ggcacggttg
    39961 tggcaatgct tgccgcctgc tcttccaaac caaccgatcg cggacagcaa tataaagacg
    40021 ggaaatttac ccagcctttc tctctggtga accagccaga tgccgttggc gcgccgatta
    40081 acgccggtga ttttgccgag caaattaacc atatccgtaa ttcgtcaccg cgtctgtatg
    40141 gcaaccagag taatgtttat aacgcggtgc aagagtggct gcgcgcaggc ggtgataccc
    40201 gcaatatgcg ccagttcggc attgatgcct ggcagatgga aggtgccgac aactatggta
    40261 acgtgcagtt tacaggctat tacacgccgg taattcaggc gcgccatacc cgccagggcg
    40321 agttccagta tcctatttac cgtatgccgc caaaacgtgg tcgtctgccg tctcgtgcgg
    40381 agatctacgc aggggcgctg agtgataaat atattctcgc ttacagcaac tccctgatgg
    40441 ataacttcat tatggatgtg cagggtagcg ggtatatcga ctttggtgat ggcagtccgc
    40501 ttaacttttt cagctatgca gggaaaaacg gtcatgccta tcgcagcatt ggtaaggtgc
    40561 tgatcgaccg tggcgaagtg aaaaaagaag atatgtcgat gcaggcgatt cgtcattggg
    40621 gcgaaacaca cagtgaagcc gaggttcgcg agctgctgga acagaacccg tctttcgttt
    40681 tctttaaacc gcaatctttt gcaccggtga aaggggcaag tgcggtgccg ctggttggcc
    40741 gtgcttctgt tgcctccgat cgttcaatta ttccgccagg tactaccttg ctggcagaag
    40801 tgccgctgct ggataataac ggcaaattta acggtcagta cgaattgcgt ctgatggtgg
    40861 cgctggatgt cggtggcgca atcaaaggcc aacacttcga tatctatcaa gggatcgggc
    40921 cggaagccgg acatcgcgca ggttggtaca accactatgg acgtgtctgg gtgctgaaaa
    40981 ccgccccggg cgcaggtaac gtctttagcg gctgatgtgt tattctgtat accgttttta
    41041 cggatacgac agggtgaggg ataacctcac cctttttaat tctgaggtgc tatgtctgtg
    41101 gtaattagtg atgcatggcg tcagcgtttt ggtggcacag cgcgtctgta tggtgaaaaa
    41161 gcgttgcaac tgtttgctga cgcgcatatt tgtgtggttg gtatcggtgg tgtcggttcc
    41221 tgggcggcgg aagcgctggc gcgcacgggg attggcgcaa tcacgcttat cgatatggat
    41281 gatgtgtgcg tcaccaatac caatcggcaa attcacgctc tgcgcgataa cgtcgggctg
    41341 gcaaaagcgg aagtcatggc ggagcgtatt cgccagatta acccggagtg ccgtgtaacg
    41401 gtggtggatg atttcgtgac gccggataac gtagcgcagt atatgagcgt gggttattcg
    41461 tacgtgattg atgccattga tagtgtacgg cccaaagcgg cgctgattgc ttattgtcgg
    41521 cgcaataaaa tcccgctggt cacgaccggt ggcgcgggtg ggcagattga cccgacgcag
    41581 attcaggtta ccgatctggc gaaaacgatt caggatccgc tggcggcgaa gttgcgcgag
    41641 cgcctgaaaa gcgattttgg cgtagtgaaa aacagtaaag gtaagctcgg cgtcgattgc
    41701 gtgttttcta ctgaagcgct ggtgtacccg cagtcagacg gtactgtgtg tgcgatgaaa
    41761 gccacggcag aagggccgaa gcggatggat tgtgcatctg gatttggcgc ggcaacgatg
    41821 gtgaccgcca cctttgggtt tgttgcggtt tctcatgcgc tgaagaagat gatggcgaaa
    41881 gcggcgcgtc agggttaaac aatcaggtcg gatgcgacgc gaacgtctta tccgacctgg
    41941 ttctgtgctt aaacctgctt cgccgcagcg ataatcgcct cgcttaacgc attcaacccc
    42001 tggctgcgtg aggcgctaag ctgtgcacgc aatcccagct catcaaacaa tgccagcggc
    42061 gactgtgcct gcaattcggc ggcggttttc ccctcaacgg cagtcaacaa caccgccaac
    42121 aggccgcgca caatgcgccc ttcgctgtcg ccaaagaagt gcattttacc gttttcagcc
    42181 acggtatatc ccagccagac gcggttttcg catccggcaa tctctttagc ctgcgctttt
    42241 aactcgtctg gcaaagccgg aagctgtttc cccagcatga tcaactggcg atatttatct
    42301 tcccattgcg acaacggagc gaaggtattg cgtaacgttt ctgcggttac ggttgtgccg
    42361 aacggatgtc cggcgaattg cgggtttgtc attaatccac caataattcc agcgcgcggt
    42421 caacggcatt caccagcgca tccacatcac tctttgtatt atatggcgca aatgaggcgc
    42481 gcagtgtgcc ggttacgcct aattctgcca gtagcggctg agcgcaatgt tgcccggccc
    42541 gcaaggcaat accgtactcc gccagtagcg tcaccatatc gctgtgatga acgccagcaa
    42601 aataaaaggc cagcaggctg gaatcctggc agcggaatga acgaaagcct ggacgtttcg
    42661 ccagcgcttc ttctgcaagc gttgctaagc tacggctcca gctttcggcc tggttgatat
    42721 cgtaatctgc cagccattcc agcgccgcgc ttaatcctat gacgccagcg acatttggcg
    42781 ttcctgcttc cagtttccac ggcgcagatt gagtggtgaa accgtcaaaa ctcacttcgt
    42841 gaatcatttt gccgccaccc agccagggcg acatcgcttc cagcagttct gatttgccat
    42901 acagcacgcc gatacccgtc gggccataca gtttgtgacc agagaaagca tagaaatcga
    42961 tatccagttg ctgaacatct gcggggaaat gcaccgcccc ctgagcacca tcaaccatca
    43021 ccaccatccc ggctgaatga gcaaaggtaa tcgctcgcac cagatccggg caaccgccag
    43081 taacgttcga catctgaccc aacgccagaa tccgactacg gggagtaatc agttctggca
    43141 acaaatcgac atccggcagt cgctgcgcat taagcggcaa tttcaccact ttggctccag
    43201 tttgttgggc gaccatcagc caggggacga ggttagcgtg gtgttctgcc acgctgacaa
    43261 taatctcatc gcccggttgc agacgcggac gcgcatagca ttgtgccacc atgttgatgg
    43321 attcagtggt gccgcgcgtc cagacgatag ttttatcatc cggtgcattc agtaattgcg
    43381 ccactttctc tcgtgcagct tcataacgcg cggtcaggcg ttgggcttcg gcaaactggc
    43441 tgcgatggac gtttccggcg ctcagactgt aaaactgttg ggtggcttca accacggctt
    43501 caggtttaag cgcggtcgcg gcgctgtcga gatagacgcc cgcatcctgt agtgcgggaa
    43561 actgggcgcg aaactgcgcg ggattaaaaa cgttcatggt actcctcggc ttgatgcacc
    43621 gatcgtgacg caaaattcac tttgatacaa ggagagtgct aaccatcaga attatctgga
    43681 tatcctggcg aagccgcgta agtaacatat gctgaatagt gttaggcgtt aattttagcc
    43741 tgttaatcaa tactattaag atttattaat cgctttaagg agaagatgat gaaaaagact
    43801 gccgcaatta tttctgcctg tatgctgact tttgccctga gcgcctgttc cggttcgaac
    43861 tatgtgatgc acaccaatga cggacgtacc atcgtctctg acggcaaacc acagactgat
    43921 aacgataccg gtatgatttc gtataaagac gctaatggca acaaacagca gatcaaccgt
    43981 actgacgtga aagagatggt cgaactggat cagtaattcg cgatcgcaag gtaaaaaaaa
    44041 gcaccgcaat taggcggtgc tacattaatc actatggaca gacagggtaa atgtacagga
    44101 agtgaaaaaa ggtagctttg ctaccatggt ctgaatcgca gaccaattgc aaacacaaca
    44161 acacaacatc acaaccgtaa gccaaaagtt caccagaaca cgcattccga taaaactttt
    44221 cgttccggct caggaagtgc cgccactata ggtatttgct ggtagaagct caacggacaa
    44281 tttataatgg ctcagattaa aaa
//
LOCUS       DAZKHC020000030        44203 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZKHC020000030 DAZKHC020000000
VERSION     DAZKHC020000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 44203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.030"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..447
                     /locus_tag="ACHOSM_RS09670"
                     /old_locus_tag="pgaptmp_001934"
     CDS             <1..447
                     /locus_tag="ACHOSM_RS09670"
                     /old_locus_tag="pgaptmp_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4558472.1"
                     /translation="EVIATPEASKNLKAAWNARKKELKLTQELAAELLGFESQGTVSQ
                     YLNGKIPVNTDAALKFAALLKVKPEDIREDLKDLMNYVRSSDTYDDSFSGKGWRLVNE
                     EQAELLNLFEILPASEKAKLLNQLRGLNKLYEEAFENMLALKKRNQ"
     gene            complement(790..1020)
                     /locus_tag="ACHOSM_RS09675"
                     /old_locus_tag="pgaptmp_001935"
     CDS             complement(790..1020)
                     /locus_tag="ACHOSM_RS09675"
                     /old_locus_tag="pgaptmp_001935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4558473.1"
                     /translation="MLENQTLKVACPDCGSEMLKRPDDFDFDTNFVGVSCAECGREIT
                     KDDVIKQGKNVVQKQVNDMLRNTLKGSGWKFK"
     gene            1461..1682
                     /gene="kilR"
                     /locus_tag="ACHOSM_RS09680"
                     /old_locus_tag="pgaptmp_001936"
     CDS             1461..1682
                     /gene="kilR"
                     /locus_tag="ACHOSM_RS09680"
                     /old_locus_tag="pgaptmp_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HGU4558474.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITDRMIKVFLGRDGLPVKAESW"
     gene            1682..1852
                     /gene="ydaE"
                     /locus_tag="ACHOSM_RS09685"
                     /old_locus_tag="pgaptmp_001937"
     CDS             1682..1852
                     /gene="ydaE"
                     /locus_tag="ACHOSM_RS09685"
                     /old_locus_tag="pgaptmp_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HGU4558475.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     SCAEKDQMAHEL"
     gene            1927..2202
                     /gene="racC"
                     /locus_tag="ACHOSM_RS09690"
                     /old_locus_tag="pgaptmp_001938"
     CDS             1927..2202
                     /gene="racC"
                     /locus_tag="ACHOSM_RS09690"
                     /old_locus_tag="pgaptmp_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein RacC"
                     /protein_id="HGU4558476.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            2304..4904
                     /gene="recE"
                     /locus_tag="ACHOSM_RS09695"
                     /old_locus_tag="pgaptmp_001939"
     CDS             2304..4904
                     /gene="recE"
                     /locus_tag="ACHOSM_RS09695"
                     /old_locus_tag="pgaptmp_001939"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HGU4558477.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSIDAKYQELRAELHEARKNIPSK
                     DPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGVSKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNAGKEEE
                     KAFLMECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETG
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQQEYHRNLRTLSDCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            4897..5706
                     /gene="recT"
                     /locus_tag="ACHOSM_RS09700"
                     /old_locus_tag="pgaptmp_001940"
     CDS             4897..5706
                     /gene="recT"
                     /locus_tag="ACHOSM_RS09700"
                     /old_locus_tag="pgaptmp_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HGU4558478.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNSDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSLSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            5763..5957
                     /gene="ralR"
                     /locus_tag="ACHOSM_RS09705"
                     /old_locus_tag="pgaptmp_001941"
     CDS             5763..5957
                     /gene="ralR"
                     /locus_tag="ACHOSM_RS09705"
                     /old_locus_tag="pgaptmp_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HGU4558479.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKVISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            5950..6138
                     /locus_tag="ACHOSM_RS09710"
                     /old_locus_tag="pgaptmp_001942"
     CDS             5950..6138
                     /locus_tag="ACHOSM_RS09710"
                     /old_locus_tag="pgaptmp_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HGU4558480.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            6238..6453
                     /gene="xisR"
                     /locus_tag="ACHOSM_RS09715"
                     /old_locus_tag="pgaptmp_001943"
     CDS             6238..6453
                     /gene="xisR"
                     /locus_tag="ACHOSM_RS09715"
                     /old_locus_tag="pgaptmp_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HGU4558481.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            6455..7690
                     /gene="intR"
                     /locus_tag="ACHOSM_RS09720"
                     /old_locus_tag="pgaptmp_001944"
     CDS             6455..7690
                     /gene="intR"
                     /locus_tag="ACHOSM_RS09720"
                     /old_locus_tag="pgaptmp_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4558482.1"
                     /translation="MSKLPTGVEIRGRYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTLISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNMWKFAVYSGLRHGELAALAWEDVDLEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQIAMLNARLS"
     gene            7742..8677
                     /gene="ttcA"
                     /locus_tag="ACHOSM_RS09725"
                     /old_locus_tag="pgaptmp_001945"
     CDS             7742..8677
                     /gene="ttcA"
                     /locus_tag="ACHOSM_RS09725"
                     /old_locus_tag="pgaptmp_001945"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HGU4558483.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFGAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(8806..10179)
                     /gene="dbpA"
                     /locus_tag="ACHOSM_RS09730"
                     /old_locus_tag="pgaptmp_001946"
     CDS             complement(8806..10179)
                     /gene="dbpA"
                     /locus_tag="ACHOSM_RS09730"
                     /old_locus_tag="pgaptmp_001946"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HGU4558484.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAMDDVIRFAPASRQTLLFSATWPEAITVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(10209..10382)
                     /gene="ynaL"
                     /locus_tag="ACHOSM_RS09735"
                     /old_locus_tag="pgaptmp_001947"
     CDS             complement(10209..10382)
                     /gene="ynaL"
                     /locus_tag="ACHOSM_RS09735"
                     /old_locus_tag="pgaptmp_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HGU4558485.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(10657..11640)
                     /gene="zntB"
                     /locus_tag="ACHOSM_RS09740"
                     /old_locus_tag="pgaptmp_001948"
     CDS             complement(10657..11640)
                     /gene="zntB"
                     /locus_tag="ACHOSM_RS09740"
                     /old_locus_tag="pgaptmp_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HGU4558486.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(11654..11701)
                     /gene="ydaN"
                     /locus_tag="ACHOSM_RS09745"
                     /old_locus_tag="pgaptmp_001949"
     CDS             complement(11654..11701)
                     /gene="ydaN"
                     /locus_tag="ACHOSM_RS09745"
                     /old_locus_tag="pgaptmp_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HGU4558487.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            11895..13127
                     /gene="dgcM"
                     /locus_tag="ACHOSM_RS09750"
                     /old_locus_tag="pgaptmp_001950"
     CDS             11895..13127
                     /gene="dgcM"
                     /locus_tag="ACHOSM_RS09750"
                     /old_locus_tag="pgaptmp_001950"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HGU4558488.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHMTEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(13148..13711)
                     /gene="smrA"
                     /locus_tag="ACHOSM_RS09755"
                     /old_locus_tag="pgaptmp_001951"
     CDS             complement(13148..13711)
                     /gene="smrA"
                     /locus_tag="ACHOSM_RS09755"
                     /old_locus_tag="pgaptmp_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HGU4558489.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(13906..15419)
                     /locus_tag="ACHOSM_RS09760"
                     /old_locus_tag="pgaptmp_001952"
                     /pseudo
     CDS             complement(13906..15419)
                     /locus_tag="ACHOSM_RS09760"
                     /old_locus_tag="pgaptmp_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231236.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
     gene            complement(15416..16444)
                     /locus_tag="ACHOSM_RS09765"
                     /old_locus_tag="pgaptmp_001953"
     CDS             complement(15416..16444)
                     /locus_tag="ACHOSM_RS09765"
                     /old_locus_tag="pgaptmp_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4558490.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATIAMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            16541..17356
                     /locus_tag="ACHOSM_RS09770"
                     /old_locus_tag="pgaptmp_001954"
     CDS             16541..17356
                     /locus_tag="ACHOSM_RS09770"
                     /old_locus_tag="pgaptmp_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4558491.1"
                     /translation="MTIILQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            complement(17529..17745)
                     /locus_tag="ACHOSM_RS09775"
                     /old_locus_tag="pgaptmp_001955"
                     /pseudo
     CDS             complement(17529..17745)
                     /locus_tag="ACHOSM_RS09775"
                     /old_locus_tag="pgaptmp_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            17745..17888
                     /locus_tag="ACHOSM_RS09780"
                     /old_locus_tag="pgaptmp_001956"
                     /pseudo
     CDS             17745..17888
                     /locus_tag="ACHOSM_RS09780"
                     /old_locus_tag="pgaptmp_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            17899..18414
                     /gene="ogt"
                     /locus_tag="ACHOSM_RS09785"
                     /old_locus_tag="pgaptmp_001957"
     CDS             17899..18414
                     /gene="ogt"
                     /locus_tag="ACHOSM_RS09785"
                     /old_locus_tag="pgaptmp_001957"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGU4558492.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGLNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            18609..19361
                     /gene="fnr"
                     /locus_tag="ACHOSM_RS09790"
                     /old_locus_tag="pgaptmp_001958"
     CDS             18609..19361
                     /gene="fnr"
                     /locus_tag="ACHOSM_RS09790"
                     /old_locus_tag="pgaptmp_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HGU4558493.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            19513..20463
                     /gene="uspE"
                     /locus_tag="ACHOSM_RS09795"
                     /old_locus_tag="pgaptmp_001959"
     CDS             19513..20463
                     /gene="uspE"
                     /locus_tag="ACHOSM_RS09795"
                     /old_locus_tag="pgaptmp_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HGU4558494.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(20513..20770)
                     /gene="ynaJ"
                     /locus_tag="ACHOSM_RS09800"
                     /old_locus_tag="pgaptmp_001960"
     CDS             complement(20513..20770)
                     /gene="ynaJ"
                     /locus_tag="ACHOSM_RS09800"
                     /old_locus_tag="pgaptmp_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HGU4558495.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            21014..22045
                     /gene="ynaI"
                     /locus_tag="ACHOSM_RS09805"
                     /old_locus_tag="pgaptmp_001961"
     CDS             21014..22045
                     /gene="ynaI"
                     /locus_tag="ACHOSM_RS09805"
                     /old_locus_tag="pgaptmp_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HGU4558496.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(22096..23709)
                     /gene="mppA"
                     /locus_tag="ACHOSM_RS09810"
                     /old_locus_tag="pgaptmp_001962"
     CDS             complement(22096..23709)
                     /gene="mppA"
                     /locus_tag="ACHOSM_RS09810"
                     /old_locus_tag="pgaptmp_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HGU4558497.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(24046..24945)
                     /gene="pgrR"
                     /locus_tag="ACHOSM_RS09815"
                     /old_locus_tag="pgaptmp_001963"
     CDS             complement(24046..24945)
                     /gene="pgrR"
                     /locus_tag="ACHOSM_RS09815"
                     /old_locus_tag="pgaptmp_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HGU4558498.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            25083..26003
                     /locus_tag="ACHOSM_RS09820"
                     /old_locus_tag="pgaptmp_001964"
     CDS             25083..26003
                     /locus_tag="ACHOSM_RS09820"
                     /old_locus_tag="pgaptmp_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4558499.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            26003..26935
                     /locus_tag="ACHOSM_RS09825"
                     /old_locus_tag="pgaptmp_001965"
     CDS             26003..26935
                     /locus_tag="ACHOSM_RS09825"
                     /old_locus_tag="pgaptmp_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4558500.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            26953..27591
                     /locus_tag="ACHOSM_RS09830"
                     /old_locus_tag="pgaptmp_001966"
     CDS             26953..27591
                     /locus_tag="ACHOSM_RS09830"
                     /old_locus_tag="pgaptmp_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4558501.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTEEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            27900..28628
                     /gene="mpaA"
                     /locus_tag="ACHOSM_RS09835"
                     /old_locus_tag="pgaptmp_001967"
     CDS             27900..28628
                     /gene="mpaA"
                     /locus_tag="ACHOSM_RS09835"
                     /old_locus_tag="pgaptmp_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HGU4558502.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(28603..29568)
                     /gene="ycjG"
                     /locus_tag="ACHOSM_RS09840"
                     /old_locus_tag="pgaptmp_001968"
     CDS             complement(28603..29568)
                     /gene="ycjG"
                     /locus_tag="ACHOSM_RS09840"
                     /old_locus_tag="pgaptmp_001968"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HGU4558503.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            29687..30190
                     /gene="tpx"
                     /locus_tag="ACHOSM_RS09845"
                     /old_locus_tag="pgaptmp_001969"
     CDS             29687..30190
                     /gene="tpx"
                     /locus_tag="ACHOSM_RS09845"
                     /old_locus_tag="pgaptmp_001969"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGU4558504.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLR"
     gene            30347..32389
                     /locus_tag="ACHOSM_RS09850"
                     /old_locus_tag="pgaptmp_001970"
     CDS             30347..32389
                     /locus_tag="ACHOSM_RS09850"
                     /old_locus_tag="pgaptmp_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4558505.1"
                     /translation="MDDKQITFWLNDNGCSADIPAIAEALTNHAEWLLELAPDPIEEG
                     SSCLPPAAAAGIFLGAAAMVHCGETSGAETWLEAAITDYHFFNPNGHSSWRGSTPVFT
                     AISRYPALRMVLFNAACAMEDWNKTSTVLESLFHASYVTEDDPAAPNFTPYALKAFIA
                     DNHPLGPAHYDEIWLLAKQAWLINAGVLDERTCNTWMQYTRHLRHLIDNEQFADALAF
                     VRSKKEPLNHIHTYSDFYLYAIGLFSSTGKLSEALTWVKQLIRNNDSHFYDLFVSTGK
                     ERRIKPELTTLLNNLLHSAEFQALQDKYLTGEYGVVHSGPFMSVYEKVLGGKSRKRCA
                     ISRKLISPGEVVYKYRHVDTVEYIAAKAAFQASELNNIAHRHENNSYQWQDFAARWPR
                     RGSLSPPDIARYLFERQEGKCFDAAEFIQLIGEPFVFPMRFIWVAGLSFELHQYPDAY
                     FVNDNMAGEFVNLCWMAMKCGHAGDIFQQLAQEPHDVADPIYAMLATFDRADCRSAAA
                     AHFGQPEIAEIMALAFSSRLSLDSVLTIAEFGKNQPRFSHALATALLRYNLHIYSNYM
                     PQVNWFLQGLEHYALAKGGQLLNFFVHIPEHIPVLATMLEHGVLVRGIGEGAYDGYHN
                     SANSFHHAAVMHCLAHAPEKVRYWMDPPWIEHYLLKAPLRQTARYVEAWHKKFGIK"
     gene            complement(32427..33968)
                     /gene="tyrR"
                     /locus_tag="ACHOSM_RS09855"
                     /old_locus_tag="pgaptmp_001971"
     CDS             complement(32427..33968)
                     /gene="tyrR"
                     /locus_tag="ACHOSM_RS09855"
                     /old_locus_tag="pgaptmp_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HGU4558506.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKGEE"
     gene            complement(34116..35177)
                     /gene="ycjF"
                     /locus_tag="ACHOSM_RS09860"
                     /old_locus_tag="pgaptmp_001972"
     CDS             complement(34116..35177)
                     /gene="ycjF"
                     /locus_tag="ACHOSM_RS09860"
                     /old_locus_tag="pgaptmp_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HGU4558507.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(35174..36571)
                     /gene="ycjX"
                     /locus_tag="ACHOSM_RS09865"
                     /old_locus_tag="pgaptmp_001973"
     CDS             complement(35174..36571)
                     /gene="ycjX"
                     /locus_tag="ACHOSM_RS09865"
                     /old_locus_tag="pgaptmp_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HGU4558508.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            36726..37724
                     /gene="ycjW"
                     /locus_tag="ACHOSM_RS09870"
                     /old_locus_tag="pgaptmp_001974"
     CDS             36726..37724
                     /gene="ycjW"
                     /locus_tag="ACHOSM_RS09870"
                     /old_locus_tag="pgaptmp_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4558509.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCERKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(37835..38740)
                     /gene="ompG"
                     /locus_tag="ACHOSM_RS09875"
                     /old_locus_tag="pgaptmp_001975"
     CDS             complement(37835..38740)
                     /gene="ompG"
                     /locus_tag="ACHOSM_RS09875"
                     /old_locus_tag="pgaptmp_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HGU4558510.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     HLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(38785..39867)
                     /locus_tag="ACHOSM_RS09880"
                     /old_locus_tag="pgaptmp_001976"
     CDS             complement(38785..39867)
                     /locus_tag="ACHOSM_RS09880"
                     /old_locus_tag="pgaptmp_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4558511.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKTRNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(39881..40540)
                     /gene="pgmB"
                     /locus_tag="ACHOSM_RS09885"
                     /old_locus_tag="pgaptmp_001977"
     CDS             complement(39881..40540)
                     /gene="pgmB"
                     /locus_tag="ACHOSM_RS09885"
                     /old_locus_tag="pgaptmp_001977"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HGU4558512.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPLTESLTWPRLSAFWQN
                     V"
     gene            complement(40537..40800)
                     /locus_tag="ACHOSM_RS09890"
                     /old_locus_tag="pgaptmp_001978"
                     /pseudo
     CDS             complement(40537..40800)
                     /locus_tag="ACHOSM_RS09890"
                     /old_locus_tag="pgaptmp_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
     gene            complement(41158..41769)
                     /locus_tag="ACHOSM_RS09895"
                     /old_locus_tag="pgaptmp_001979"
     CDS             complement(41158..41769)
                     /locus_tag="ACHOSM_RS09895"
                     /old_locus_tag="pgaptmp_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4558513.1"
                     /translation="MDKAEKTVSSAERFIDMEGINRFSERLAMAMGNMTNVELATLCG
                     LSEATVRNYRKGKNFPPLDKLALIAKACNCSLEWLATGKNEDKCGKIENNCDSGSGID
                     QALSSLSPQEKQLILTFIRREGTNNLVRLAAMNTSTISQDAVESIIDALPLRPVLKNA
                     IKIGMAGSEETDKEILRVIEKYNSSNNATQESGAVTVKKNVVG"
     gene            41927..42193
                     /locus_tag="ACHOSM_RS09900"
                     /old_locus_tag="pgaptmp_001980"
     CDS             41927..42193
                     /locus_tag="ACHOSM_RS09900"
                     /old_locus_tag="pgaptmp_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4558514.1"
                     /translation="MRVSRQASNNLFVREGCDWSAKAVYAALENKGTDLPELERELGL
                     KSGSMRNVFYRQCRKYEAAIAEKIGVEPAVIWPSRYQVDAREVA"
     gene            42204..>44203
                     /locus_tag="ACHOSM_RS09905"
                     /old_locus_tag="pgaptmp_001981"
     CDS             42204..>44203
                     /locus_tag="ACHOSM_RS09905"
                     /old_locus_tag="pgaptmp_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HGU4558515.1"
                     /translation="MWVTAKECVGLPDLPMMEHNIRHRLTKAAAGNAAMLRKRKGTKA
                     IEFNIDCLPSGAQQVLRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVMKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPVKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVA"
BASE COUNT        11990 a        10847 c        10369 g        10997 t
ORIGIN      
        1 gaagtgattg caactccaga agcgagcaag aatttaaaag ccgcatggaa tgcaagaaaa
       61 aaagagctga agctgactca agagttggcg gctgagttgt tgggattcga atctcaaggc
      121 accgttagcc agtatctgaa cggcaagata ccggtaaata ccgacgctgc gctaaaattt
      181 gcggctctgt taaaggtaaa accagaggac attcgagaag accttaaaga cttaatgaat
      241 tatgtaagat catcagatac ttatgatgat agcttttcag gcaaaggatg gaggctggtc
      301 aatgaagaac aggcagagtt acttaacctc ttcgagattc tacctgcgtc agaaaaagcc
      361 aaactcctta accagctacg tggactaaac aagctctacg aggaagcctt cgagaacatg
      421 ctggcactaa agaaacgtaa ccagtagcca ccgctcacta ccccacccac aacaaaaaaa
      481 aaccgacgtc ttagtcggtt ttttttgtgc cataacttct gcaaatcagc tgtataacta
      541 atatttttcc cttgaaaaaa catttacata gttacaaaat caaaaatata atacgccttg
      601 ctgttgactt aaaatatacg tgtcactaat atttctatcg agaacagcac ggcgctgtag
      661 gttttagttc cgccacccgg cgttaaggga aaagatttga ggtgatgtat gacgggagtg
      721 aaaatagcgc ctatcaaact taccatagac gctacagaag taacggaaca ctttagtgcg
      781 atacatgggt tatttaaact tccacccgct gcctttgagg gtgttccgga gcatgtcatt
      841 gacttgtttc tgaactacat tttttccttg cttaataaca tcgtccttag tgatttctcg
      901 accacattcg gcacaactaa cgccaacgaa attagtgtca aagtcaaaat catcgggccg
      961 cttgagcatc tcactgccgc aatcagggca ggcaaccttc aaggtctgat tttcgagcat
     1021 agaaaaatcc ttccatctgt tgggggatat tcagattaac ctaattttcg ctgttgggga
     1081 ataacgaaaa ccaccgaagc ccggaggtgg tgaaataaaa ccgggcacaa cacgaaggcg
     1141 catttccgat atccataaag agtcggtctt gtctgttaaa tttaaatggt gggagtgcgc
     1201 ctccggttgt aaataacgac attgctgtgt gtagtcctgg cggcatcagt tttttcttga
     1261 agttcggctg atgtccgccc ttttttaagt gaattttgtg atgcggtgaa tgcggctaag
     1321 cgcacgtggc acagttaaaa gtcatgttag tccttattgg tttgggtggg aaagccgact
     1381 gtaattgtta actggttgca gtcacctgga ggcaccaggc accgcatcaa caaagttcat
     1441 ttgtaaaaat ggagataatt atgattgcac atcacttcgg aactgatgaa ataccacgtc
     1501 agtgtgtgac tcctggcgat tatgttcttc atgaaggccg gacatatatt gcctcggcaa
     1561 acaatattaa aaagcgaaaa ctatatattc gtaacctgac cacaaaaaca tgcattactg
     1621 accgcatgat taaagtcttc ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt
     1681 gatgactaag aaaataaaat gtgcttacca cctttgcaaa aaagacgttg aagaaagcaa
     1741 agctattgaa agaatgcttc acttcatgca cgggatttta tcaaaagacg aaccgagaaa
     1801 atattgcagt gaatcttgtg ccgaaaaaga ccagatggca catgaacttt aattaattga
     1861 ctattcgaaa ctgaatttat gccagaaatg gcaggtattc gctcaacctt aattaaggag
     1921 aaaaacatga ttaccaatta tgaagccact gttgtaacta ccgatgacat tgttcacgag
     1981 gtgaatctgg aaggaaagcg cattggctac gtaattaaaa cagaaaataa agaaacccca
     2041 ttcactgtgg ttgatatcga tggtccatca ggcaacgtaa aaacacttga tgaaggtgtc
     2101 aaaaaaatgt gcctggtgca tatcggaaag aatctgcccg cagaaaaaaa agccgaattt
     2161 ctggcaactc taattgcaat gaaattaaaa ggtgaaatct gaaagaaata gcctgcgtat
     2221 ggcgcaggct atgaacagtg tgtatccggc aagatcattc actgaacaaa acgaatttta
     2281 atctgagttg aggttaaaaa acaatgagca caaaaccact cttcctgtta cggaaagcga
     2341 aaaaatcatc cggtgaacct gacgtcgtcc tgtgggcaag caacgatttt gaatcgacct
     2401 gtgccactct ggactacctg atcgttaagt caggtaaaaa actgagcagc tattttaaag
     2461 ctgttgccac gaattttcct gtcgttaatg acctgcccgc tgaaggtgag atcgatttta
     2521 cctggagtga acgctatcaa ctcagcaaag actccatgac atgggaacta aaaccgggag
     2581 cagcaccaga caacgctcac tatcaaggca ataccaacgt caacggcgaa gacatgactg
     2641 agattgagga gaatatgcta ctcccaattt ctggccagga actgcccatt cgttggcttg
     2701 ctcaacacgg cagcgaaaaa ccggtaacgc acgtttcacg cgacggactc caggcattac
     2761 acattgctcg ggctgaagaa ctaccggctg ttactgccct ggctgtttcc cacaaaacca
     2821 gcctgctcga cccgctggaa attcgcgaac tccacaaact ggttcgtgac actgacaaag
     2881 ttttccctaa tcctggtaat tcaaacctgg gactgataac tgcttttttc gaagcatacc
     2941 tgaacgctga ctacaccgat cgaggactgc tgacaaaaga gtggatgaag ggtaatcgtg
     3001 tttcacacat cactcgcacg gcttccggtg ctaatgctgg cggcggaaac ctcaccgatc
     3061 gcggcgaagg tttcgtacac gatctgacgt cactggcgcg cgacgtagcc actggcgtac
     3121 tggcccgttc aatggatctg gacatctata accttcatcc ggcacacgct aaacgcattg
     3181 aggaaattat cgctgaaaat aaaccgccct tttctgtttt ccgcgacaaa ttcatcacca
     3241 tgcctggcgg gctggattat tcccgcgcca tcgtggttgc gtccgtaaaa gaagcaccaa
     3301 ttgggatcga ggtcatcccc gcgcacgtca ctgaatatct gaacaaagta ctgactgaaa
     3361 ccgatcatgc caaccctgat ccggaaatcg tggatattgc ctgcggtcgc tcctctgccc
     3421 caatgccgca gcgagtaaca gaagaaggaa aacaggatga tgaagaaaaa ccgcaaccat
     3481 ctggaacaac ggcagttgaa cagggagagg ctgaaacaat ggaaccggac gcaactgaac
     3541 atcatcagga cacgcagccg ctggatgctc agtcacaggt aaattctatt gatgcgaaat
     3601 atcaggaact gcgggcagaa ctccatgaag cccggaaaaa cattccatca aaagatcctg
     3661 tcgatgccga taaattgctt gctgcatcac gtggtgaatt tgttgacgga attagcgacc
     3721 cgaacgatcc gaaatgggta aaggggatcc agactcgcga ttgtgtgtac cagaaccagc
     3781 cagaaacgga aaaaaccagc ccggatatga atcaacctga gccagtagtg caacaggaac
     3841 cggaaatagc ctgcaatgcc tgcggccaga ctggcgggga taactgccct gactgtggtg
     3901 cggtgatggg cgacgcaaca taccaggaaa cattcgatga agagagtcag gttgaagcta
     3961 aggaaaatga tccggaggaa atggaaggcg ctgaacatcc gcacaatgag aatgctggca
     4021 gcgatccgca tcgcgattgc agtgatgaaa ctggcgaagt cgcagatccc gtaatcgtag
     4081 aagacataga gccaggtatt tattacggaa tttcgaatga gaattaccac gcgggtcccg
     4141 gtgtcagtaa gtctcagctc gatgacattg ctgatactcc ggcactatat ttgtggcgta
     4201 aaaatgcccc cgttgacacc acaaagacaa aaacgctcga tttaggaacc gctttccact
     4261 gccgggtact tgaaccggaa gaattcagta accgctttat cgtagcacct gaatttaacc
     4321 gccgtacaaa cgccggaaaa gaagaagaga aagcgtttct gatggaatgc gcaagcacag
     4381 gaaaaacagt tatcactgcg gaagaaggcc ggaaaattga actcatgtat caaagcgtta
     4441 tggctttgcc gctggggcaa tggcttgttg aaagcgccgg acacgctgaa tcatcaattt
     4501 actgggaaga tcctgaaaca ggaattttgt gtcggtgccg tccggacaaa attatccctg
     4561 aatttcactg gatcatggac gtgaaaacta cggcggatat tcaacgattc aaaaccgctt
     4621 attacgacta ccgctatcac gttcaggatg cattctacag tgacggttat gaagcacagt
     4681 ttggagtgca gccaactttc gtttttctgg ttgccagcac aactattgaa tgcggacgtt
     4741 atccggttga aattttcatg atgggcgaag aagcaaaact ggcaggtcaa caggaatatc
     4801 accgcaatct gcgaaccctg tctgactgcc tgaataccga tgaatggcca gctattaaga
     4861 cattatcact gccccgctgg gctaaggaat atgcaaatga ctaagcaacc accaatcgca
     4921 aaagccgatc tgcaaaaaac tcagggaaac cgtgcaccag cagcagttaa aaatagcgac
     4981 gtgattagtt ttattaacca gccatcaatg aaagagcaac tggcagcagc tcttccacgc
     5041 catatgacgg ctgaacgtat gatccgtatc gccaccacag aaattcgtaa agttccggcg
     5101 ttaggaaact gtgacactat gagttttgtc agtgcgatcg tacagtgttc acagctcgga
     5161 cttgagccag gtagcgccct cggtcatgca tatttactgc cttttggtaa taaaaacgaa
     5221 aagagcggta aaaagaacgt tcagctaatc attggctatc gcggcatgat tgatctggct
     5281 cgccgttctg gtcaaatcgc cagcctgtca gcccgtgttg tccgtgaagg tgacgagttt
     5341 agcttcgaat ttggccttga tgaaaagtta atacaccgcc cgggagaaaa cgaagatgcc
     5401 ccggttaccc acgtctatgc tgtcgcaaga ctgaaagacg gaggtactca gtttgaagtt
     5461 atgacgcgca aacagattga gctggtgcgc agcctgagta aagctggtaa taacgggccg
     5521 tgggtaactc actgggaaga aatggcaaag aaaacggcta ttcgtcgcct gttcaaatat
     5581 ctgcccgtat caattgagat ccagcgtgca gtatcaatgg atgaaaagga accactgaca
     5641 atcgatcctg cagattcctc tgtattaacc ggggaataca gtgtaatcga taattcagag
     5701 gaataattca gcctggcggt gtaatgcacc gccaacttga aatatttttt atgagaaaaa
     5761 ttatgagata tgacaatgtt aaaccatgtc cattttgtgg ttgtccatca gtaacggtga
     5821 aagtcatttc aggatattac cgagcgaagt gtaacggatg cgaatcccga accggttatg
     5881 gtggaagtga aaaagaagca ctcgaaagat ggaataaacg aaccactgga aataataacg
     5941 gaggtgttca tgtataaaat taccgccact attgaaaagg aaggtggcac tcctactaac
     6001 tggacaagat attcaaaatc taaactaacg aaatcagaat gcgaaaaaat gctctcaggt
     6061 aaaaaagaag caggcgtttc cagagagcag aaagtaaaac tgataaattt taattgcgag
     6121 aaacttcagt cctcgtgaat tgcattgtat tcaaattaaa acttcatagc tgattattaa
     6181 taatcaacat cgggcgtcaa tttcagtcta acattggcgc ctgccagagg tgatgcgatg
     6241 gcacaagtaa tctttaatga agagtggatg gttgaatacg gcctgatgct tcgcactggt
     6301 ctgggggcca gacaaattga agcataccgc cagaactgtt gggtggaggg cttccacttc
     6361 aaacgagtat ctcctttagg taagccagac agcaaacgag ggattatctg gtacaactat
     6421 ccaaagataa atcagtttat caaagactca tgatatgtct aaattaccaa caggtgtcga
     6481 gattagaggt agatacattc gcatctggtt catgtttcga ggaaaacgat gtcgggaaac
     6541 attaaaaggc tgggagatta caaacagtaa tattaaaaag gccggaaatt taagagcgct
     6601 gatagttcat gaaataaact ccggtgaatt tgagtattta agacgttttc cccagtccag
     6661 cactggggca aaaatggtga caacgagagt cataaaaacg ttcggagagc tttgtgatat
     6721 ctggacaaaa attaaagaga cagagttaac aacaaacaca atgaagaaaa cgaaatcaca
     6781 attaaaaaca ctcagaataa taatttgtga aagtaccctg atatcacata ttcgttatag
     6841 cgatatctta aactaccgga atgaactgct gcatggagaa acgctttacc tggataatcc
     6901 aagatccaac aaaaaaggaa gaaccgtgcg cacagttgat aactatatcg ccctgctctg
     6961 ttcgctgtta cgttttgcgt atcagtcggg atttatatca accaaaccat ttgaaggagt
     7021 aaaaaaatta cagcgaaaca gaataaagcc tgatccgtta tctaaaacag aattcaatgc
     7081 attaatggaa agtgaaaaag gacagagcca gaacatgtgg aaatttgcag tttactcagg
     7141 acttcgtcac ggggaactgg cagctctggc gtgggaggat gtggatctcg aaaagggaat
     7201 agtgaatgtc agaagaaacc tgacgatact tgatatgttc ggtcccccaa aaacaaatgc
     7261 cgggatccgg acagtaacac tactgcagcc tgctcttgaa gcactgaagg agcaatacaa
     7321 actgaccggg catcatcgca aaagcgaaat caccttttat catcgggagt acggcagaac
     7381 cgaaaagcaa aaactgcatt ttgttttcat gcccagggtg tgtaacggaa aacaaaaacc
     7441 ttattactcg gtaagcagtt tgggggcaag gtggaatgca gcagtaaaac gtgctggtat
     7501 tcgccgccgt aatccgtacc atacgcggca tacttttgcc tgctggctgt tgacggcagg
     7561 agcgaacccg gcatttatag ccagccaaat ggggcatgaa actgcgcaga tggtgtatga
     7621 aatttacggt atgtggattg atgacatgaa cgacgaacag atagccatgt tgaatgcgcg
     7681 gttatcatag ttgcaaagtt tgcccccaat ttgccccatt tagtaccaga gaactgaaat
     7741 aatgcaagaa aatcaacaaa ttacaaagaa agaacaatac aacctgaaca aattacaaaa
     7801 acgtctgcgt cgtaacgtgg gcgaagccat tgctgacttc aatatgattg aagaaggcga
     7861 tcgcatcatg gtttgcctct cagggggtaa agacagctat accatgctgg agattctgcg
     7921 caatttgcag caaagcgcgc caatcaattt ttcgctggtg gctgttaacc tcgatcaaaa
     7981 gcaaccgggc ttcccagaac acgttctgcc cgagtatctt gaaaagctgg gcgttgagta
     8041 caagattgtc gaagagaaca cttacggtat cgtgaaagag aagattccag agggcaaaac
     8101 cacttgctca ctgtgttctc gccttcgtcg cggtatcctt tatcgtaccg caacggaact
     8161 gggggcgacg aagatcgcgc tgggtcacca tcgtgacgat atcctgcaga cgttgttctt
     8221 aaatatgttc tacggcggca agatgaaagg tatgcctccg aaactgatga gcgatgatgg
     8281 caaacatatc gttattcgtc cgctggccta ctgccgcgag aaagatattc agcgatttgc
     8341 cgatgcaaaa gcgttcccga ttattccgtg caacctgtgc ggttcacagc ctaacctgca
     8401 acgtcaggtg attgctgaca tgttgcgtga ctgggataaa cgttatccag ggcgtatcga
     8461 gacgatgttc ggcgcgatgc agaatgtggt gccgtcgcat ctgtgcgata ctaacctgtt
     8521 cgatttcaaa ggcatcaccc acggttctga agtggttaac gggggtgatc tggcgtttga
     8581 tcgcgaagag atcccactac aaccggcggg ctggcagcca gaagaagatg aaaatcagtt
     8641 ggatgagtta cggctgaatg tggttgaagt gaaataacca agatggcgcc cgatgcgcaa
     8701 gcgtatcggg ctactcttat ggaggccgga taagacgcgg ccagcgtcgc atccggcaat
     8761 cccgaataag atgtttactc ttgcacccgg caattcaaca tttcattatt ttaataaccg
     8821 cacccggcac gtttttcctt taatcttccc gccctgtaac tgtttccatg ctttatgagc
     8881 aacagcctga cggaccgcga catagacatg cgccggatgc acggcgattt tgccaatatc
     8941 tgcgccatca agcccgatat ctcctgtcag tgcacctaat acatcacccg ggcgcatttt
     9001 ggcttttttc ccgccatcga tacacaacgt tgccatttct gcttccagcg gcacaatgga
     9061 actattagct ggcggcgttt gccagttaag ttttatctgc aacatgtcag aaatgatatt
     9121 ggcccgctgt gcttcttccg gagcacagaa actgatcgcc agaccgctat ttcctgcacg
     9181 agctgtacga ccgatgcgat gtacatgaac ttcagggtcc cacgccagct caaagttcac
     9241 caccagctca agcgatttaa tatccagacc acgcgcagca acatcagtcg caaccagtac
     9301 acgggcgcta ccgttagcaa aacgtaccag agtctgatcg cgatcgcgtt gctccagatc
     9361 gccgtgtaac gacaatgcac tttgccctac ttcattcagc gcgtcgcaga cagcctggca
     9421 atcttttttg gtattgcaaa acaccacaca agaggatggc tgatgcaagc ttaataaccg
     9481 ttgcaacaga ggaattttgc ctttactgga tgtctcataa aattgttgtt caataggtgg
     9541 caaagcatct gttgagtcaa tttcaatcgc caaaggatcg cgttgcactc gtccgctgat
     9601 tacggtgatg gcttccggcc aggttgccga aaacagaagc gtctgtcgag atgcaggcgc
     9661 aaaacggatg acatcatcca tggcatcgct aaatcccata tccagcatgc ggtcggcctc
     9721 atccatcacc aaagtattca gcgcatccag tgataccgtg cctttttgca agtgatccag
     9781 caaacgaccc ggcgttgcca cgataatatg cggcgcatgt tgcaacgaat cacgctgcat
     9841 accgaacggt tgaccaccgc acaacgtcaa aattttggta tttggcagaa aacgcgccag
     9901 ccgacgcaat tcacctgcca cctgatccgc cagttcacgc gtaggacaca gcactaaagc
     9961 ctgggtttga aatagcgacg catcaatttg ctgtaacaag ccgaggccaa aagccgccgt
    10021 tttgccgctg ccggttttcg cctgcacgcg aacatctttt ccggcaagga tcgccggaag
    10081 cgcggcggcc tgcaccggcg tcatggttaa ataacccaac tcattaaggt tcgtgagttg
    10141 ggcgggaggc aaaacattca gggtagaaaa agcggtcaca atctattctc gtggtcatcg
    10201 acgcaaagtt agcaggcgcg tatcctcgca gatctacgct cacgatgcga caatttaatc
    10261 ggttcttcat caggtggtgg gtcaggcatg ggttgcgggc gagggatcgg atcgggcact
    10321 ggaacaggat cgccaggaat cggttcaggg acaggaattt gcaaataaat aagtgtcgtc
    10381 atatttccct ccggtcattg ggtggactct taaagggtag acgctgataa ataacaggca
    10441 aaaaaaagcc gactcatcaa agtcggcgtc gtacgaatca attgtgctat gcagtaattc
    10501 aaaaaaggaa gtaagacaat atggagcgca acgcccatcg cttgacgttg cattcacctg
    10561 caagggagat attgccctga atgggtagag agtttattga cttcgctcaa actttgcggc
    10621 gtttttgtat acagacagcc ggaaaaattg cttttgttac aaccatttac tacggtgcaa
    10681 ccataaagca acaccaccaa taagaacaac taacagaata caaaaaattg aaaatccgaa
    10741 ttgccacccg ccgccaggga tcccaccaag gttgacgcca aataacccgg tcagaaaggt
    10801 actgggtaaa aagaccattg ccatcaacga cattgtatag gtacgacgag ctaaattttc
    10861 ctgcatcacc tgagcgattt catccgccat cacgccagtc cgtgctatac aggcgtcgat
    10921 ttcgtcaagg ccgcgcccaa ggcgatcggc aatatcctgc atccgacggc gttggtcatc
    10981 gctcatccac ggcaaacgtt cactggcaag acgagcataa acatcacgtt gcggtgccat
    11041 atagcgacgc atcacaatta attgtttgcg cagcagagcc aggaatccac gcggtggaat
    11101 ttgctgatca aggagattat cttcaaggtc gataatttta tcgtgcagct gctcgataaa
    11161 ttcgctggaa tgatcggtca acgcatcgca cacatccacc agccaccctc cgcaatcggt
    11221 cggacccgtg ccctcttcca gatcgctcac cacatcgtcc agtgccagca ctttgcgttg
    11281 tcgggtcgaa acaattaacc gcccgtccat atatacacgc atggcgacca gttgatcggg
    11341 gcgttcatcg gtgctgccgt ttatacagcg caatgtaatc agcgtgcctt caccgagacg
    11401 gctgactcgg ggccgcgtgc tctcgcccgc cagcgcatca cgtacgttat tgggaagcag
    11461 cggtgttgtc gccagccatt gggcgctatc atggtgtaca taattaaggt ggagccagca
    11521 gggatgcgct tcatcaatca catctgtatt ttccagcggt ttaacgccgc ctctaccatc
    11581 cagcatccag gcaaatactg catccgggac attaacgtcc gatcccttaa tcgcttccac
    11641 agtgcctcca tcatcaacgc attattttgt agtctagcct tctggccctg ttacgcaaca
    11701 tctcatcacc ccattaccct gaaatgatta ataaaattct gtctaaattg aatacaaaaa
    11761 gcaaaatgct tttccgtata caaaccgtgt gaagtgttaa atagcgtcta tcattatcag
    11821 aattatctga tcatatgacg tggctttttt gcgatcggat agcaacaaaa attgataaaa
    11881 ataacaggat ctcaatgatt acgcacaact tcaataccct ggacttactc accagtcctg
    11941 tctggatcgt ttcgcccttt gaggaacagt taatttatgc caatagcgcg gcgcgactgt
    12001 tgatgcaaga cctcacgttt agtcagctac gaaccggtcc ctattcagtc tcctcacaaa
    12061 aagaactgcc gaaatacctc tccgatctgc aaaaccaaca cgatattatc gaaatcctca
    12121 ctgttcagcg taaagaagag gaaacagcat taagctgtcg gcttgttttg cgaaagctga
    12181 cagaagcaga accggtgatt attttcgaag gtatcgaagc gccggcaacg ctgggtttaa
    12241 aagccagtcg ctcggcaaat tatcagcgca aaaaacaagg tttttatgcg cgcttttttc
    12301 tgactaactc tgcaccaatg ttgttgattg acccctcacg agatggccaa atcgtcgatg
    12361 ctaacctcgc cgcgctcaat ttctatggtt ataaccatga aacgatgtgc cagaaacata
    12421 cctgggaaat aaatatgctc gggcgtcgcg tcatgcctat catgcatgaa atttcgcatt
    12481 tacccggtgg tcataaacct ctgaatttta ttcataagct ggcggatggt tcgactcgtc
    12541 atgtgcagac ctacgccggg ccgattgaaa tttatggcga caagctcatg ttatgtattg
    12601 tgcatgatat tactgagcaa aaacggctgg aggagcagct ggaacatgct gcccaccatg
    12661 acgcgatgac cggattactg aatcggcgac agttttatca catgaccgag ccaggccaaa
    12721 tgcagcatct cgccatcgcc caggattaca gcttgttgct catcgacacc gatcgtttta
    12781 aacacattaa cgatctctat gggcattcta aaggtgatga ggtgttatgc gccctcgccc
    12841 gcaccctcga aagttgcgct cgcaaaggcg atttggtgtt tcgttgggga ggcgaagagt
    12901 ttgtcttatt gctaccaaga accccactgg ataccgcgct ttcgctggct gaaactatcc
    12961 gcgtaagcgt ggcaaaagtg agtatttcgg gcttaccacg ctttaccgtc agcattggtg
    13021 tggcgcatca cgaaggaaat gaaagcatcg atgaactgtt taaacgcgtt gatgatgctt
    13081 tgtatcgggc gaaaaatgat ggacgcaacc gcgtgctggc ggcataagcc gcggatgcgt
    13141 ctcgagatca acgactgcgc ttagcgtggc gctcccagtt ttcttgcttc gcctgcgccg
    13201 ttttacgtag tgcgacgtaa cacgccccgc tgccgccatg atgcggtagc gcggtgcaat
    13261 atgcctgaac atcatcaaat tcggtcagcc agcgcgccac atagctgcgg acaatattgg
    13321 catgcgattt atcatcccgt cctttgccat gaataatcag cacgttacgc aaaccatcag
    13381 ccatggcttg ttgaatgaaa ctgaacatca ttttgcggca ctcttccacc ggctggcgca
    13441 aaagattcag gctcgcctgt tgcggatatt taccactgcg cagcttatcc agcaccccat
    13501 gttgcaaccc ttcccgccga aactccagcg gctgacttag tgggatgatg tcgagaaatc
    13561 cggtggtgag gaaattatca agctgcagcg tgtcgatacg ctgcggcgca cgttggttac
    13621 gcgttggatg ccagtggaca tcggtagcac gtttcagcgg ctggacatct tccatggcgt
    13681 caagaaacag cgatttgtcg tcaaggttca tgtaacctcc tatgcacctt cacacgtcgt
    13741 gattcagacg ggttaattgt tcatacttac tgcatcgttc ccttcacaga ttgaaacaat
    13801 actctacttc atacctgtga gtatctgcgc aggcggggca ttttatacta gcctgttagc
    13861 agtatcgaac agtttagccc gagtttaatt cagcacaggc tccgatcagg atgatttatt
    13921 catagcagga accaacaaaa atgcgataaa taacagacca ctgattagca gaaaggtatc
    13981 cgaaaatgcc atagtttgcg cctccctcag catcaattga ctcaataatt tggttgaagc
    14041 cagtatttcc gatgtctggt cacttccact gcgattaaaa aataaagcac ttctggaaat
    14101 gaagtcattc accgtatggg gaacagaaac cattttttca cccattcgcg aaaaatgaaa
    14161 attagttcga ttattgagaa tagagccaca cagggcaata ccaatcgcgc ccccaagatt
    14221 acgtgttaaa ttaaatacac cagaggctaa cttcagccgt tcttttggaa ttccccccag
    14281 cgttaatgtt acgatggggg ccatcgcaaa ctgctgcgaa atccctctaa ttgcctgtgg
    14341 aaacagtaat tcctgccatc cccattcgtg ggttataggg gtaaaaagat acattgaaaa
    14401 cacaaatccg cccagccccg ccatcaacag ccatcgcaga tttatttttt tacttaacca
    14461 gaagtaaaag ggaacagaaa ataactggaa aatacctgtt gtgcaaacgg caaaacctat
    14521 ctcttcggca tttaatccgc gaatctgccc caaaaacaca ggtattaaat atacagtaga
    14581 gaatatccca acccctccag aaaaagaaaa aaaacatccc agagtgaaat ttttatcctt
    14641 aaaagcatga agatccatta tcggattgga tattgtaagt gtacgcgcag caaataaaat
    14701 aaaactcacc aatgccagaa ctgatgtcag caaaatagta ttatcatcca gccatcccca
    14761 gcgcgctcct tcttcaaggg tatattccag acatcccaga gtcatggcca acaggataat
    14821 actcggataa tcagcaactt cagtaaagag agatcagatt tatcaaaatt aaccagaaaa
    14881 ggaatactca aaactaaata tatgccaggt aatatattga tataaaatag ccatcgccag
    14941 tcgaggttat cagtgatcca gccccccaga gttggcccta gcgtcggtga cagtgaggcc
    15001 agcgcactga ctaccgcagc agccaacccc aactctttac cctggtaata aataaacgcc
    15061 gtcgtgaaaa ctaaagggat catggatgcg ccagcagctc cctgtaaagc acgaaacaaa
    15121 atcatgatct gaatattcca tgcaagtcca cacgcgatac tcattaaagt aaatattccc
    15181 gcggatagag taaaaagcca acgagtggaa aatacccgtg acagccaccc agagagcgga
    15241 ataacaataa tctcagcaat taaatagctg gtctgaagcc aggccatttc gtctttacct
    15301 gcggacaaac caccacctat ttcatttagg gatgacgata caatttgaat atcaagctga
    15361 gcaataaata atcccataca catactggcg aaagcaatat gtttacgcat agacatcatc
    15421 tctcatcaac actgacggtg acggacagtc ccgggcgaag aagttgcttt aattcctttg
    15481 cgtcgtcaaa tgcaatccgt accgggacac gctgaacaat tttggtgaaa ttgccagtgg
    15541 cattttctat cggaatcaag ctgaaactgg ctcctgtcgc tggggaaagg ctttcaatgt
    15601 gaccgtgaaa cacttcccct ttgagaatat cagcacggat ctcagccttc attcctggct
    15661 tcattcccgc tatttggttt tctttataat ttgcatcaac ccacaactca ctgactggta
    15721 ctagtgaaac taacgatgtg ccgccttcca cccaactgcc tgtatgggcg ctgcggtttg
    15781 cgacaatgcc atcaatcggc gcacgcacaa gggtatattc cagatccagt agtgcctgag
    15841 tcaaagcggc ctgaacttct tcgatactcg cacgtacgtt ttcttcctga gcagataaaa
    15901 ctgccagccg ttgcttctcc accaggaaat cgttcgcggc tttgcgctcc tttgctgcga
    15961 cctgctggta gtcaaacctg gcgttatcaa taatttgttg cgatattgcc gctgattgtg
    16021 cgagtttttc ataacgttca gtatctcgca ggcgcttttg ctcctcatgt tttactgcct
    16081 gccatgtttc actggcactt tgaattatcg actgctgcat cgcaatagtg gcttgaatgc
    16141 cttcgagatt ggctttgcta ctttttattt ttgcctcaag cctggcgaca tttgcacgat
    16201 aatcccgatc atcgagacgc aggataatat cgcctttttt tacgctctga ttatcgcgca
    16261 cctctatagc agaaatataa ccagaaactt tacttgccac cgtggtgata ttaccaccaa
    16321 tgtaagcatc atccgtcgtc tgtataaatc ttcctttgct tacccaccag ataaaaaaga
    16381 caacgccagt caaaagaata ccgatcgcta ccaccccaat aagctgtttt ttagaaatta
    16441 tcatcactct ttacaccgta tatatccgga aacttgcata cgtcatattt aaaatttaac
    16501 atggcttatt tgatgatttc tgacaaaata cctactgtaa atgacaatta tactgcaacc
    16561 caaagcatca ccagggcatc acattatcgg actggattcg gaacatctga atggaggtac
    16621 agttccctgg cataaacatc tctatgccca gttgctttac ccggctgaag gagtcgttcg
    16681 tgtatgggct ggagaaagcg tctggctagt ccatgccagc agcgcgcttt ggctgccccc
    16741 acaaatgccg cataagtttg ttgcgacagg caacgtactt ttaaaaaccg tactggtttc
    16801 agaagcggaa agtgaaacgc tgggaacagt ttgcttcatg acgggtattt cacccctgtt
    16861 aagggaatta ctaattgcaa ttaaccaact tccaccgtcg caaagtacca cagataaaca
    16921 acagttacgt ttttcagccc tcgagacact gatacttcag gaaataaaaa tgggggtgaa
    16981 aatgtcactt gaactccctt ggccaaatga tgagcggcta cagcagcttt gtgagaattt
    17041 gctaaacaac caagggtatc tgccaacact ggataatcta gcggataaaa ttaacgtcag
    17101 tagccgtaca ctcatgcgct tatttgttaa agagacggga ttaacatttc gccactgggt
    17161 tcagcaaatg cacgttattt ctgcagtgac gcttcttgat gatggatatt cgttgaccaa
    17221 aattgcccat cgtctgggct atgcctcagc tgaatcgttc ggcaatatgt ttaagcgtcg
    17281 aacgggctac tcgcccggta aatttactcg tcgattaacg atgcatgatt atgcaatcac
    17341 aagacaaatg atttaactta tattttcgtg aaatctgtca ctgaagaaaa ttggcaacgg
    17401 atgggtaaaa ccgttataac actgtcaccg gtcacaagga ccccaggccg gatttaagac
    17461 gaggatgcac tgctgtgtgt actgtagagt ctggcggatg tcgacaggct ctattttttt
    17521 attcattttt agctctgtaa ataaccacat tctcgccggg attggggaat gagtgacgag
    17581 ttcagtggtg tctggcggct atcacgccac agaatcaaat aacagatagc ctacggtaaa
    17641 atttcactga ctggaattat cccaccctcg atccatgtga tggcttgccc tcatttcttc
    17701 aggcagaaaa atcaaaaaat cgctttatgc tatcagactg cgacaaacca gacgcgaaac
    17761 tgggtactta ctattcgtta gtcttgccct atccgcttat ctttttggtg gtatggctgc
    17821 agatgttgct ggcgtggtat cttgttggtc tgccgatagg tccggggatt tacccacgtt
    17881 tgtcttaaga gagaacggat gctgagatta cttgaagaaa aaattgccac gccactgggt
    17941 ccactgtggg tgatttgcga tgagcaattt cgcctgcggg cggttgaatg ggaagagtac
    18001 agcgaacgca tggtgcagct gctggacatc cattatcgca aagaaggcta tgagcgcatt
    18061 tctgccacca acccaggcgg tttaagcgac aagcttcgtg attattttgc cggtaatctt
    18121 agcattattg atacgcttcc cactgccaca ggggggacgc catttcagcg cgaagtctgg
    18181 aaaactctac gcactatccc ctgcgggcag gtaatgcatt acggccaact ggctgaacaa
    18241 ttgggccgcc ctggcgcggc gcgtgccgtt ggtgcggcaa acggattgaa tcccatcagc
    18301 atcgtcgtac cttgccatcg ggttattggc cgaaacggca ccatgaccgg atatgcaggc
    18361 ggagttcagc gaaaagagtg gttattgcgc cacgaaggtt atcttttgct gtaaacatta
    18421 aacaatttgt gccagcttgt tcacactttt atgtaaagtt acccttaaca acttaagggt
    18481 tttcaaatag atagacatat atttacatct aatatcggaa ttctctgctg ttaaggtttg
    18541 cttagactta cttgctccct aaaaagatgt taaaattgac aaatatcaat tacggcttga
    18601 gcagacctat gatcccggaa aagcgaatta tacggcgcat tcagtctggc ggttgtgcta
    18661 tccattgcca ggattgcagc atcagccagc tttgcatccc gttcacactc aacgaacatg
    18721 agcttgatca gcttgataat atcattgagc ggaagaagcc tattcagaaa ggccagacgc
    18781 tgtttaaggc tggtgatgaa cttaaatcgc tttatgccat ccgctccggt acgattaaaa
    18841 gttataccat cactgagcaa ggcgacgagc aaatcactgg tttccattta gctggcgacc
    18901 tggtgggatt tgacgccatc ggcagcggcc atcacccgag cttcgctcag gcgctggaaa
    18961 cctcgatggt atgtgaaatc ccgttcgaaa cgctggacga tttgtccggt aaaatgccga
    19021 atctgcgtca gcagatgatg cgtctgatga gcggtgaaat caaaggcgat caggacatga
    19081 tcctgctgtt gtcgaagaaa aatgccgagg aacgtctggc tgcattcatc tacaacctgt
    19141 cccgtcgttt tgcccaacgc ggcttctccc ctcgtgaatt ccgcctgacg atgactcgtg
    19201 gcgatatcgg taactatctg ggcctgacgg tagaaaccat cagccgtctg ctgggtcgct
    19261 tccagaaaag cggcatgctg gcagtcaaag gtaaatacat caccatcgaa aataacgatg
    19321 cgctggccca gcttgctggt catacgcgta atgttgcctg atttttccgc ataactcact
    19381 atccttctgt catatcatta aatttttctg atttattgat ctggcagaag gttcatcact
    19441 gtttcattca ccagatatgg gttaatcttt taattacaaa ctgcgttgac agttgttgta
    19501 aggagaccct gtatggctat gtatcagaac atgctcgttg ttatcgatcc taaccaggac
    19561 gaccaaccag cattgcggcg agctgtttat ttacatcaac ggattggtgg caaaattaaa
    19621 gcctttttgc cgatctatga cttctcatac gaaatgacca ccctgctctc cccggacgaa
    19681 cgtaccgcta tgcgtcaggg cgtcatcagc cagcgtacag cctggatcca cgagcaggca
    19741 aaatattatc tcaatgctgg cgttcccatt gaaataaaag tggtctggca taaccgtcct
    19801 ttcgaagcca tcattcagga agtgatcagc ggcgggcacg atttggtgct aaaaatggcg
    19861 caccaacatg accgtctgga agcggtgatt tttacgccaa cggactggca tctgttacgc
    19921 aaatgcccaa gcccggtgtg gatggtgaaa gaccagccgt ggccggaagg aggcaaggcg
    19981 ctggtggcgg tgaatctcgc cagtgaagag ccgtatcata atgcgctcaa tgaaaaactg
    20041 gtcaaagaga cgatcgaact ggcagaacaa gtcaaccata ccgaagttca tctggttggc
    20101 gcttatccgg taacgccaat taatatcgcg attgaactgc cggaatttga tccgagcgtt
    20161 tataacgatg ccattcgtgg gcaacatttg ctggcaatga aagctctgcg gcagaaattc
    20221 ggcattaatg aaaacatgac gcacgtagaa aaaggtctgc cagaagaggt gattcctgat
    20281 ttggcggagc acttacaggc ggggattgtg gttctgggca cggtaggacg caccggtatt
    20341 tcagcagcat tcctcggcaa cacggcggaa caggtgattg atcatcttcg ctgcgatctg
    20401 ttggtcatta aacctgacca gtatcagaca cccgttgaac tggatgacga agaagacgat
    20461 taacgactct tcaataactg acaaggcccg agttatcggg cctatttttt acttactgct
    20521 cttcgccgcc aaggaaataa attcccaacg ggattgccag caacacagta aagaccaggc
    20581 tataaacaaa aatcatcgat gactgaatca catacattga tgtcgtccac tcagacagcg
    20641 gaatattgta ctgttctata acgccgccga tagtcgcgcg agtcacaacc gacgccgcaa
    20701 taatgaaaac cgccaacaaa ccaaagagaa atttcttccc tttcgctgac ttcagtttcg
    20761 ccataatcat gatgtaatcc ttgtgcagac aaatttcttg ttacctaaac gtaacaatac
    20821 catgacattt caccgttgtc tgctctgaaa acctcacctc ttagctggat ttacaatcaa
    20881 cgcattgatt agttagctaa tgttgctact tccttccctg actattgatg atctgaatga
    20941 attgtcgcgg taaaatagcc ccgccagggg tttccggcat gacgacaatt atctggagga
    21001 gcctgataaa acaatgatcg ctgaactgtt tacaaataat gcgcttaatc tggtcattat
    21061 tttcggtagc tgcgcagcat tgattctgat gagcttttgg tttcgccgcg gaaatcgtaa
    21121 aagaaaaggt tttttattcc atgcggtgca atttttaatc tacaccataa ttatcagtgc
    21181 tgttggtagc attattaatt atgttataga aaactataaa ctcaaattta tcaccccagg
    21241 cgttatcgat tttatctgta cgtccctgat tgcggttatt ctgacgatta agctattttt
    21301 gctgattaat cagtttgaaa aacagcagat taaaaaaggt cgcgatatca ccagtgcgcg
    21361 gattatgtcg cgcattatca aaatcaccat tattgtggtg cttgttctgc tttatggcga
    21421 acatttcggc atgagccttt ctggcttgct gacctttggt ggtattggtg gtctggctgt
    21481 cggtatggcc ggtaaagata ttctgagtaa tttcttttcc gggatcatgc tctatttcga
    21541 ccgtcctttc agtattggcg actggatccg ttcaccggac agaaatatcg aaggcacagt
    21601 agcggaaatt ggctggcgaa ttaccaaaat tacgaccttt gataatcgtc cattgtacgt
    21661 accgaactcg ctgttttcgt cgatcagcgt agaaaaccca ggacgaatga ccaaccgccg
    21721 cattaccacg accattggtt tacgttatga ggatgcggca aaagtgggcg ttattgtcga
    21781 agccgtacgt gagatgctga aaaatcaccc ggccatcgac cagcgacaaa ccttactggt
    21841 ttatttcaac cagtttgccg actcgtcatt gaatattatg gtttattgct ttaccaaaac
    21901 cacggtatgg gctgaatggc ttgctgcaca gcaagacgtt tatttgaaga ttatcgatat
    21961 tgtgcagtca cacggcgcgg actttgcctt ccccagccag acgctgtata tggataacat
    22021 tacaccgccg gaacaggggc gctaaatcag acatcactgg cgttatccac aagggtaacg
    22081 ccagttccca tcacatcaat gcttcacaat atacatagtc cggctgtacg ccacatcttc
    22141 cgggttatta atgggataac ctttcagcca cggcttgatt aatcgtccat tggtatattg
    22201 ataaattggt gcaatcggtg cttgctccat gaggattttt tctgccgcgt tgtaatcggc
    22261 attacgcgct ttaacggtat tttccgtcga tgcctgggcc agaactttgt cgtatgccgg
    22321 attgttaaag cgtgaaatat ttcctgaatg cgttgacgtt aataatgtca ggaaagtgga
    22381 gggttcatta taatccccca cccatgaggc acggataaca tcaaaattgc cggtgttacg
    22441 gctatcgata taggttttcc attcctgatt ttgcaattta acatctacgc caaggttctt
    22501 tttccacatc gatgctacag caatcgcaat tttttgatgg ttttctgaag tgttatacaa
    22561 aagcgtcagc ttcagcggtt tttgcggacc ataaccagct gcgctcaaca aagtttttgc
    22621 ctgcgcattc agttcttcct gactcatttg ttcaaacggc gaaggttccg gcgtaaatcc
    22681 ggcggtaaca tctggtgtaa aatgccacgc tggcttttcg cccgttccca acactttttc
    22741 ggtcatcagg cggcgatcta tcgtcatact taatgccagg cgaacgcgct gatctgccgt
    22801 cggccctttt tgcgtgttaa acgcataata ataggtcccg agctgtggcg gcgtataaac
    22861 ctgccccgga atatccttca acagcttctg atacatattt ttcgggaagg attcggtgat
    22921 atcaatatcc cccgcgaggt aacgcttagt ggctgcggat tcctgattaa ttggcaggaa
    22981 ggtcactttt tgcagtaccg ttttggcgtt atcccaataa tgggtattcg gtaccacgac
    23041 cagtttttca ttgactacgc gatctttaag aacataagcg ccattgccga tcagatttcc
    23101 gggtttcgtc cactctttac cgctttctac gttggctttt tgcaccggga agaaggcaaa
    23161 gttagcggtt aaattcacaa accacggcaa cggtttatca agctgaattt tcaaagtatg
    23221 ggcatcaact gcggtgacgc caagctggtc tggcgtagct ttaccatcaa taatcgcctg
    23281 tgcgttgttg attcccgcca gcgcggcaaa ccatgcaaat ggcgatagcg ttttcgggtc
    23341 caccagacgt tgccagctgt agacaaaatc ttgcgccgtt accggtgtgc catccgccca
    23401 ttttgcgtta tcgcgcaggg taaaagtcca gatacggttg tcattacttt tccactgagt
    23461 cgcaacgccg gggacaatct cccctttctc gttctgattc accagtcctt caaacagatc
    23521 gcgaatgacc tgaatctctg gcaggcccac ggctttagcg ggatccagcg acgcaggctc
    23581 atctttaatg tggcgcacca gctcctgctt ctctgccagt actgtgccgc tcggaacttc
    23641 tgcagcatac gaaagagaaa tgctgctgac caacagcgca caacacgtga ctgaaacaga
    23701 gtgcttcata agtttccctc taaccgacat taaaattgat gcgtaattat ttgtttcgcc
    23761 ttaaagaaat gcaaacaact tccatcaaaa aagtgacaca ggaagcataa aatgttttat
    23821 gtatcaatat attaaatcag atttagtgta aataatcgac gccaaaatgc ggcaaagcac
    23881 atagaacacc gaacactacg ttcaggcttc gcacgttcaa accagttttc ggtatcaaaa
    23941 tcagcatctg tcgccattgt acgaagaata aaaaataacc aaaacgttga aaggaactga
    24001 gaatacgtta agagagaaat taaattaatg tatcagcaag tagtgctaaa cagcaccttt
    24061 atacttcagc ctatctataa ataatgagaa tgccgagcca gcatgtcgac ggtgtggata
    24121 gtacaggtga taaccgggta aatctggtgt gaatttatcc aaaacacgta tcagtttttt
    24181 ttctttaata gcccgctcaa cctgatcata aggtagatac gccaatccat gcccatcaat
    24241 tgcagcatca atgatcaggt ctatcgtatt cagtaaaagc tgaccttcca tgcgaacacg
    24301 aacttcacgt ccaccgcgta ttaatctcca gcgatttgct gtacccgatg tgggaagata
    24361 caaattaatt gcctgatgat ctattaattg tgacactgac gttggaacac ttcggcgaga
    24421 aaaataatcc ggtgagccaa caatagccat tggtatatct ggcccgattc gaatggcgat
    24481 catatcttta tccatctccc cccccagacg gacgcctgca tcaaaacgtt cagaaacgac
    24541 atcggtcaaa ccataatcaa tggtgagctg aatatcaatt tcaggatgcg atttcaggaa
    24601 tgtgcgcatt gctggtaaca atatcgtttt tgctgcatgt tctacagtag taatacgtat
    24661 tgtcccggat gggcggttct gcagctcgct cagggatgcc agggctgaat ctatgtcatg
    24721 caacataggg ccaagaacag acaaaagatg ctcgcccgct tcagttggaa caacgctgcg
    24781 cgtggttcgc gtcagaagcc gcaatcccaa tcgttcttct atacgacgca ctatctggct
    24841 taaagctgac tgcgccatgc tcaggcgggc tgctgcacga gtgaagctac gctcctctgc
    24901 aacgacgaca aacgccatca gatcagcgat ttcttcacgc ttcattcagt atctccatgc
    24961 aatctattaa tcatttaaat gataggtcta atcatgattt tgggtctaat caacttaatg
    25021 atcaatatct atagtgatat caccaaccgt taaaacgaac cgacaactcc atgaggaacg
    25081 gaatgatgaa taataaagtc agcttcacta acagcaataa tccaaccatc tctttgtctg
    25141 cagtgatcta tttccctcca aaatttgatg aaacccgtca gtatcaggca attgtggtat
    25201 ctcacccagg tgggggcgtt aaagaacaaa cggccggaac ctatgccaaa aaactggcgg
    25261 aaaagggatt tgtcactatt gcttatgacg catcttatca aggtgaaagt ggcggcgagc
    25321 cgcgtcagtt agaaaaccca tacattcgta ctgaagacat cagtgccgtg attgattacc
    25381 tgacaacgct ttcttatgtc gacaataccc gaattggtgc gatgggaatt tgtgccggtg
    25441 caggatatac agccaatgct gcaattcagg atcgccgcat caaggccatc ggtaccgtta
    25501 gtgcggtcaa tattggttcg atgttccgta atggttggga aaacaatgtg aaatctattg
    25561 atgccctgcc atacgttgaa gcgggttcaa acgcccgaac cagcgatatt agcagcggag
    25621 aatatgccgt tatgccactg gcaccaatga aagagtccga tgcgccgaac gaagaactgc
    25681 gtcaggcctg ggagtattac cacacccctc gcgcgcagta tccaacagca ccgggttacg
    25741 ctactttgcg cagccttaac cagattatta cctatgatgc ttaccatatg gcggaagtgt
    25801 acctgactca gccgatgcag attgtggcgg gcagccaggc agggagcaaa tggatgagtg
    25861 acgacctgta cgatcgggca tcaagccaag ataaacgcta tcacattgtt gaaggcgcaa
    25921 accatatgga tctttatgac ggcaaagcct atgtggctga agcaatttca gtattagctc
    25981 cgttctttga ggaaactctg taatgaaaca tattcaaatc cgcaatagtg atatggcctg
    26041 gcatattgct gccaatattc agtttccacc aaattttgat gagtcaaaac agtatccggc
    26101 gattatcagc gtccatcctt ttggcagttg taaggagcag acgtctggca atatctatgg
    26161 caaagcattg gctgaaaaag gctatctcgt tctcgcttat gacgccagtt tccagggaga
    26221 atctggtggg gagcctcgct ggatagaaga tccgacacag cgcgtagagg atattagccg
    26281 tgttatcgat tatgcagtaa ctctgccata tgttaatgct gaacgtattg gcgtgctggg
    26341 agtgtgtggg ggtggtggct atgccattaa tgccgcgctg acagaaaaac gaattaaagc
    26401 agtagtgagt attacgggtg ttaatatcgg gcgtcttttt cgggaaggtt tcagcaatta
    26461 cgatccgatt ggcgcattga atgcaatggc gtcgcaacga accaaagagg ctcgtggtgg
    26521 agaattacag atcaacgaat tacttcccgc cagcctggat gcagcaaaag cccatggact
    26581 gaccgaacgc gatgtttatg aagcaactga ctactataaa acaccacgcg ggcaacaacc
    26641 gggaggtgcg acaaaaatgt tgttctcaca tgcacagaaa acgctggcct gggatgcctt
    26701 cgcatttact gaagtgttac tgactcagcc ggtgatggta gtagttggtg aaaaagtcgg
    26761 tgccttcggt gcttatcgtg atggactgga agtctatggc cgggcgatgg tttcgcagga
    26821 tcgtcagtta gtttcattac ccgatttctc acattatgaa ctgtatgaca aaccggaagc
    26881 agttcaggag gcgctggaaa aagttattcc ttttttcaac acccatctgg gataagtttc
    26941 atgagaaata ttatgaaaaa tgtactgatt cttggtgctg gcggtcagat agcccgccat
    27001 gtgattaatc aactggcaga taaacaaacc attaaacaaa ctttatttgc ccgtcagcca
    27061 gcaaaaatcc acaaacccta tccaactaat agcaagatta ttatgggaga tgtactgaat
    27121 catgcagcac tgaagcaggc catgcaggga caggacgttg tgtatgccaa ccttacggag
    27181 gaagatttag atattcaggc aaattcagtg atcgctgcga tgaaagcctg tgatgttaaa
    27241 cgtctgatct ttgttctttc cctcggtatt tatgatgaag ttccaggaaa atttggcgaa
    27301 tggaataatg cagttattgg tgagccccta aaaccattcc gcagggctgc ggatgcaatt
    27361 gaggcttctg gactggaata tacaattctt cgccctgcct ggctgactga tgaagatatt
    27421 attgattatg agcttacctc acgcaatgaa ccattcaaag gtacgatcgt atcacgtaag
    27481 agtgtcgcag cattaatcac agatatcatc gataaacctg agaaacacat tggtgagaat
    27541 ataggtatca atcaacccgt gactgatggt gacaagccat tattcatgta aatcaattac
    27601 atagttacca tcgtaatgta ctatgtgcag tacgatttat taaacttatt aaggtgaact
    27661 gatcaatccg atgcgattaa catgtgtaga accagtaaga ataaaatacg ccatgtatat
    27721 aacattacga cttacatcta agtcagcata attctaatat ctgcacatca ataaaaatgc
    27781 aaacaacttc cggcaaaaaa gtgatacaag atcgatgaca tagcgttgaa gaaaaggatt
    27841 tgataatccg caaaatttgc acaacactgc ctgtaatact tcctgaacca gagacgatca
    27901 tgaccgtaac ccgcccacgc gccgaacgcg gcgcatttcc gcccggaaca gaacattacg
    27961 gacgttcatt attgggcgcg ccgttaatct ggtttcctgc ccctgcagcc agccgcgaaa
    28021 gtggtttgat tctggctggc actcacggtg atgaaaactc ttcggtcgtc acgctctcct
    28081 gtgctctgcg gacgttgaca ccttctttgc gccgccatca tgtggtgctg tgtgtgaatc
    28141 ctgacggctg ccagctgggg ttacgggcca atgctaatgg tgtggattta aacagaaact
    28201 ttccggcggc gaactggaag gaaggtgaaa cggtttatcg ctggaacagc gccgctgaag
    28261 aacgtgatgt cgtattactg acaggtgaca aacctggctc agaacctgaa acccaggcgc
    28321 tgtgccagct tatacaccgc attcaacctg cctgggtggt ctcattccac gatccactgg
    28381 cctgtattga agatcccaga cacagcgaat taggcgaatg gctggcccag gcgtttgaat
    28441 tgcctctggt aaccagcgtc ggttatgaaa cgcctggctc tttcggcagt tggtgtgcag
    28501 atctcaactt gcattgcatc accgcagagt ttccgcccat ctcctccgac gaagccagcg
    28561 aaaaatatct ctttgcgatg gcaaacttgc tgcgctggca tcctaaagat gcaattcgcc
    28621 cgtcgtgaac tgaagcgccg gttccacatc taccgccagc caggtcggtc cgtcaagatc
    28681 ggcgaaactg acctgcggca ccagcggtaa agcggcgcta atggcacgag aggtacacaa
    28741 catgcagccc agcatcagac tgaaaccttg tgcacgcgct tcagtcgcca gcgccagcgc
    28801 ttccgtcaga ccaccggttt tatcgagctt aatgttaacc atctcatagc gccctttcag
    28861 cgccttcaga ttgctacgtg tatgacaact ctcatcagca caaatcggca acggatgaat
    28921 aaaattctcc agcgccgcat cgtcctgcgc aggaaggggt tgttcaagca tcgcaacgcc
    28981 taaatccgcc aatagctggc aacgcgccgc caacccttct gcacgccagg attcatttgc
    29041 atcaacgatc agcgtcgcat cgggcacagc tgtgcgaatt gccaccatcc gctcactaat
    29101 aagatggtta tccagcttca ctttcagtaa tttcgcgcct gcctgccaga gtgttgatgc
    29161 actattggcc atctgatcag gcgtaccgat gactaccgtc tgtgcagtga taactgtctc
    29221 gggaagcgtt atgccgatca aatcagccag cgattgctgc tgttttcgcg cggccagatc
    29281 ccacaatgca caatccagcg cattacgtgc tgcgccggca gggagaattt tttgcaactc
    29341 ctcccgtgtc agccctttct ctaattgcga cacgacgctc ataatttgcg ccattaccga
    29401 ggcatcactt tctccataac gcggatacgg cgtgcattcg ccggtgcctt taataccctc
    29461 ttcttccagt tcaaccacca ccacgcgcgc ttcactgcga cttccccggg caatcacaaa
    29521 cggggtatgt aagggccagg cttcctcaaa taccttaacg gttctcatct ttactccttg
    29581 ttgtcgcggt ggtctgcgga ttaagcgcaa atagcgtttg ctgtgttatt gacagttagc
    29641 ataaactagg tgtgacgtta actatatgta aacaggaaag ataatcatgt cacaaaccgt
    29701 tcatttccag ggcaacccgg ttacagtcgc caattccatc ccgcaggcgg gtagcaaagc
    29761 gcagactttt actctcgtgg caaaagatct gtctgacgtc accctcggtc agtttgcggg
    29821 taaacgcaaa gtgctgaaca ttttcccgag tattgatacc ggtgtttgcg ccgcatcagt
    29881 acgtaagttt aaccaactgg caaccgagat cgacaacacc gttgtgctat gtatttctgc
    29941 cgatctgccg ttcgcccagt ctcgtttctg cggcgcagaa ggtctgaaca acgttatcac
    30001 cctctccact ttccgtaacg ctgaatttct gcaagcctac ggtgtggcaa ttgctgatgg
    30061 cccactgaaa ggtctggcag cacgtgccgt tgtggttatt gacgaaaatg acaatgtgat
    30121 tttcagccag ctggtggatg aaatcaccac cgagccggat tacgaagcag ctctggctgt
    30181 actgcgctaa acaactgtgc cgttcactga atcaccaccg gtgaacggca gtttttgatc
    30241 taccagtccc actattttcc agaattcctg ccactttgcc ccctaaccgt tgtgcattaa
    30301 gattgctctt tgccattatt ggtccagcgg agtgattatt attattatgg acgataaaca
    30361 aattacgttc tggcttaacg acaatggctg ttcagcggat attcccgcca ttgccgaggc
    30421 gctaaccaac catgcagaat ggcttttaga gctagcgcca gatcccattg aagaaggtag
    30481 ttcatgttta ccgccggcag ctgcagccgg tatttttctt ggcgcagcgg cgatggttca
    30541 ctgcggcgag accagtggtg cagaaacctg gctcgaagca gccattactg attaccattt
    30601 ttttaatcca aacggacata gttcatggcg aggcagtacg cctgttttta cagcaatatc
    30661 gcgctacccg gcattaagaa tggtgttatt taacgccgct tgcgcaatgg aagactggaa
    30721 taaaacatcc actgttcttg aatcgctttt ccatgccagc tatgtaacgg aagatgaccc
    30781 cgccgcccct aattttaccc cttatgcgct gaaagcattt atcgcggata accacccgtt
    30841 aggacctgcg cattatgatg aaatctggtt gctggcaaaa caagcgtggt taattaatgc
    30901 gggcgttctg gatgagcgaa cctgcaatac ctggatgcaa tatacccgcc atttgcgcca
    30961 tttgatcgac aacgaacagt ttgccgatgc actcgcgttt gtacgcagta aaaaagaacc
    31021 attaaatcat atccatacct actcagattt ttatctctat gcgattggtt tattttcttc
    31081 tactggtaag ctaagtgaag cgctaacctg ggtaaaacag ttaatccgca acaatgatag
    31141 tcatttctat gatcttttcg tttcaactgg caaagagcgc cgaataaagc ctgaacttac
    31201 taccttgttg aataatctct tgcatagcgc agagtttcag gcgttgcagg ataaatatct
    31261 caccggcgaa tacggtgttg tgcattccgg tcccttcatg tcagtttatg aaaaggttct
    31321 tggcggtaag tcccgaaaac gctgcgctat tagtcgaaaa ttaatctcgc ccggcgaagt
    31381 ggtttataaa tatcgtcatg tggatactgt ggaatatatt gcagcgaaag cggcctttca
    31441 ggcatctgag ctaaataata tcgcccaccg acatgaaaat aatagttatc agtggcagga
    31501 ttttgccgcc cgatggcctc gtcggggttc actttctccc ccggatattg cccgttattt
    31561 gtttgagcgc caggaaggaa aatgctttga tgccgcagag tttattcaac tgattggaga
    31621 accgtttgtc tttccgatgc gtttcatatg ggttgccggg ttgtcctttg aattacatca
    31681 gtacccggat gcatatttcg tgaatgacaa tatggcgggt gaatttgtta atctctgctg
    31741 gatggcgatg aaatgcggac atgctggcga catttttcaa caactggcgc aggagccgca
    31801 tgacgttgcc gaccctattt acgccatgct cgccacattt gaccgtgctg attgccgtag
    31861 cgccgccgcc gcgcactttg gacaaccgga gattgcggaa ataatggcgc tggctttcag
    31921 ttcgaggtta tcgctcgaca gcgtccttac catcgctgag tttggtaaaa atcagccgcg
    31981 cttttcacat gcgttggcaa cggccctcct gcgttacaac ctgcacatct actcaaacta
    32041 tatgccgcag gttaactggt tccttcaggg actggaacac tatgctttgg caaaaggcgg
    32101 tcaactttta aatttctttg tgcatattcc agaacatatt cccgttctgg cgaccatgct
    32161 ggaacatggc gtattagtac gggggatcgg tgaaggtgcc tatgatggtt atcataattc
    32221 agcaaattca tttcatcatg ccgccgtgat gcactgcctt gcacacgcgc cggaaaaagt
    32281 ccgctactgg atggaccccc cctggattga gcactattta ctgaaggcgc cactgcgtca
    32341 aacggcgcgc tatgtggaag cctggcataa aaagtttggc atcaaatgaa cgttgccgct
    32401 gcgcctgaac ggcgcagcca gcataatcac tcttcgcctt tcttctggct taagccatat
    32461 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
    32521 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
    32581 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
    32641 ggcaacaaaa tatcctgtgg acgaagctca taaccgtcca gttgtgtcag tgcgcgatag
    32701 atagcgttct ttaactgccg cacatttccc ggccacgcat atcgcgtaag tacagtattc
    32761 aggtcagcgg ccagtttcgg acgcggcacg ccctgctcgt cggcaaagcg ggcgacgaac
    32821 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
    32881 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
    32941 agattcttct gcgtagcgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
    33001 acccgacgga aagtgccatc attaagaaaa cgcagtaatt tcgcctgcat ccgtggtgac
    33061 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
    33121 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
    33181 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
    33241 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
    33301 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
    33361 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
    33421 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
    33481 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
    33541 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
    33601 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
    33661 tccactttgc ttttcatatc gacagagagc acaggttcgg gcaacgcctc cagtaacgcg
    33721 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
    33781 cctgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
    33841 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
    33901 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
    33961 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
    34021 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
    34081 ggcgtaaaga tatccggtca gcacccgata ttgcattatt tttcgctggg cgttttgcct
    34141 ttttgcagcg tttctttcac ctgaccgata agctgacgac ggaaatcccc gaggcgaggt
    34201 ttgtcatcgt caatccacgg cagcgggcgg caaagctcca tagctttaat cccgagtcgt
    34261 gccgtcagaa gtccagcacc aattccctga gctgcgcggg tagacaaacg agcagcgaga
    34321 tcttgcgaca tccagtccat ccccacttcg cgcaccagtt cgctggctcc ggcaaaagcg
    34381 atattcagca ataccagctt aaacagacgc aaacggctgt aataccccag ttcaatgcca
    34441 tacagcgtgg cgatgcgatt aattaaacgc agattgcgcc aggcgataaa cgccatatcg
    34501 accaacgcca gcgggctgac cgcaatcatc aacgttgatt ccgccgccga acggctgatt
    34561 tcgcgccgcg cctgggcatc taaaactggc tggaccaaat gcgcatacag gcttaccact
    34621 tcacggtcgt tttgcgtttc atggattgag gcataccagc gttgcagcgc cggatgcgac
    34681 tgatcaatac ccgcctgctg cgccagtttt tcgcaaaatg cgcggccttt gcccgtgccg
    34741 tggctatgca ataaatcacg cgcttcgtcg cgttcatggg cgcgctgtcg caagcgccat
    34801 aagcgccgcc actctgttac cacagaacct acgccagcgc cgataatcaa tgccccagcg
    34861 gcacatccac ccagcgccac ccagtcctga gtttgccagg cattcattgt ccactgtaca
    34921 ccctgcccga caacgcttgc gccaaacaga gccagcccgc ccatcaccat tttgcgccac
    34981 aggctgcgtt tcggacgtaa cgctgcatcc attaccgctt caacttgccc ctcttcttca
    35041 ggcgcttcat cgagcgtggc cggggcaaaa ttttgcgcct gattttcgtc aaaggtctgc
    35101 tgcgccctga attttggatt ctgatcgacc tccagcggac cgtcgaaatc aatacgtggt
    35161 tttaacggtt cggtcatcgc aatttatctc ctattaaaaa ttccagcgcg gcatcaagac
    35221 gaatatgcgg cagcggtttg tcgacatcca tcacctgcgg gcgaaacgct tcaaactgga
    35281 acccttgctt gtcccagaac gcctgaccgg gcaaacgcgc aggaacttca ccggggtaaa
    35341 cggtgagcgg cgcaccatcg ctgagccggt taccgcgcaa tgccggaatt ttctcgccgt
    35401 tgacgtcaat aatgccgctg gtggtcgcct gcaccgaggc cagccccagg caatccatac
    35461 taatcccttc aaacgccgca ttttgccagg catcctgaat cagttgttgc agtaatgaaa
    35521 ccatattggc gtgctgatcg accgttacat ggtccgcttt cgttgcggca aacagcagtt
    35581 tgtcgataac cggcgagaat aagcggcgaa agagcgtgcg ctgcccgtag tggaaacttt
    35641 gcatcagctg cgtcagtgcc agacgcatat cattaaatgc ctgtggccca ctgttgagag
    35701 gttgcaggca atcaaccagc acaatctggc ggtcaaagcg cagaaaatga ttcttataga
    35761 accccttcac caccttctcg cagtaataat taaaccgctc gcgcagcatt ccggcattgg
    35821 tgtgcttatc ggcctgcgcc agtttggact cgccccaggt atcgacatcc ggccacggga
    35881 agaattgcag cgcgggcgca cctgccatat ctcccggcaa gacaaagcgc cctggctgaa
    35941 taaagtgcag cccctgctgt ttacagtggt ggagataatc ggtccacgcg gcggcaatgt
    36001 ctgccagccg gttttcgtcg gcaggtgcta gcgggtccag cccttcgcac atcattcgcc
    36061 atttgaccga ccattcgccg cgctgaccat tgagtaagcc cgtcatctgg cgtgaccagc
    36121 ttaaatagtc ctgcgccagc atcggcaggt cgagcaacca ttcgccggga taatccacaa
    36181 tctccagata cagcgtggag gtgtccttga agtggcgtag cagcgaatcg ttcgatttaa
    36241 aacgtagcgc caggcggatt tcactgacgc cgcgcgttgg cgtcggccag gcgggaggat
    36301 cgccatataa ctgcgccagt ccttcatcat atgtgaagcg cggaatgcca aagtcacgct
    36361 gaggaatgcg ttttacgccc agcaggcgct cttcacgcac cgcgcttaac agcggcaagc
    36421 gtgctccggc gtgaatattg agcaactgat tgaccatcgc agtgataaac gctgttttgc
    36481 cgctgcggct tagtccggtt acggcgaggc gcagatgtct gtcgacaccc cgattcacca
    36541 gcgcattaag ttcattttta agtcgcttca ttgctgtcct gtgctgctct gaagaccaga
    36601 aaaaaatcat tcgtaaggat atgaatgtgg gggccaggcc attgattatc aactgcccgt
    36661 gttgtcatcg acgggcgaac tccataagat gtgcagcatt cacgttcgga cggagttcct
    36721 gacctatgtc ccctactatt tatgatattg ccagggttgc aggcgtatca aaatccaccg
    36781 tatcacgcgt gctgaataag caaactaata tctccccgga agcgcgcgaa aaagtgttac
    36841 gggccattga agaattacag tatcagccaa acaagctggc ccgcgcgctg acctcttcgg
    36901 gttttgatgc cattatggtg atttctaccc gttcgaccaa aactacggcg ggtaatccgt
    36961 ttttctctga agttttgcat gccatcaccg ccaaagctga agaggaaggt ttcgacgtga
    37021 tattgcagac gtcgcacaat ccggcagaag acttacaaaa atgtgaacgc aaaattaagc
    37081 agaaaatgat taaaggcatt attatgctaa gttctccagc ggatgagtca ttttttgccc
    37141 aacttgataa atacgatatt cctgtcgtgg tgattggcaa agttgaaggt caatatgccc
    37201 atgtttattc tgtcgatacc gataattttg gcgacagcat tgcgttgacc gatgcattaa
    37261 ttgaaagtgg gcatcaaaat attgcctgcc tgcatgctcc gcttgatgtc catgtttcag
    37321 tggatcgggt aaatggttat aagcagagcc tggctgcgca taatattgca gtgcgtgatg
    37381 aatggattgt tgacggtggt tatacccatg aaacagcact gcaagccgcg agagaattat
    37441 taagccagtc accgttgcct gaggcggtgt ttgccactga cagtctgaaa ttaatgagta
    37501 tttatcgtgc ggcggcagag aaaaatattg ctattccgca gcagttagcg gtggtgggtt
    37561 atagtaatga aacgctgtcg tttattttaa cacctgcacc gggcggcatc gatgttccga
    37621 cgcaggagtt aggtcaacaa agctgcgagt tattattccg cttaatttct ggaaaaccgt
    37681 caccacagaa tattaccgtt gccacgcata tgacgctgaa ataaatacca gctaccttgc
    37741 ctgaatgccg gatgcgatgc tagcgcatct tatccggcct acatttatag atcggataaa
    37801 gtactcgcgc cgcatccggc aatttagccc attatcagaa cgagtaattt acgccgacac
    37861 ctgcataatg gaatttatca ctgtcgcctt cgtcgtgatc ctgccactca aacgcgtatt
    37921 ccagcgaaac ggaaagtccg ttctggaaat cataaccgta aaataaacct acacggttaa
    37981 aatcatggcc ttcacgttca atatcatcct gccagtccca gttactccag cgatccagcc
    38041 caatgcgcgt atacggcgtc accgagtggt tgccgagcgt cagcggcaaa taggcgcgaa
    38101 tttcttgcgt ggaaaactca ccgttattgc ggctgtcgtc catattgaag ccgcgctcga
    38161 gataatagtt cactcgcaag gcaaccgttt cgttgaaggt atattgcaga cctgtttccg
    38221 tttcgacacg ggtatcagcg taaccggtag tgttcagatg gttggcaaat ttatacatcg
    38281 acaaccaacc gttgaaacgt aaatcgtcag tcagtttcac atcccagtct ggcgcgattt
    38341 tccagcgctg catattcgcc gtgtctttac ccggttcatc aacgtagtga taaccataat
    38401 tacggaaacc gccggtcagg ccgaaactga aatcatcgtt ttcgaggaac tgataatgca
    38461 cctccagctc cgggcgatca aaccacgttc cacgtttacc cgcgctataa tctaccggcc
    38521 cttcctgata ataggccaga gcaattctcc acggcccgtt ggcggcatta aaatagactg
    38581 aaggctccgc cagcccatcc atatcttcgc cataaccctc gacgttttct atttcgtaca
    38641 tcgcgccgat attaaagtgc cagtcgttct tttcctcggc ctgtgcgcag gccattcccg
    38701 cacacatcac cagtgcggtg cagggtaata actttttcat tattgttcct tgtattattc
    38761 tgggggaatg ccttccccct gtatttagcg aattgctatt tccgtttctg catcaaagaa
    38821 atgacatttc gtcatatcaa aatgaatagt gatattttct cctgcatgat aatcatttaa
    38881 cgcaccagca cggaccacta actcgtgccc cccaaccgtg gtgtagagca taaattccgc
    38941 accggttaat tctgccacgc taattttggc ggaaatgtta ttttcctctt gcgcgtccgg
    39001 atgaatatct tccggacgta ttcccatcac gatgggctta tgcaaacttc cctgtgtttt
    39061 cagaaccgct aatttctctt cgggaatggt taatttaagc gtttccgtaa cgaatttatc
    39121 gccatcgatc gtgccacgaa taaaattcat cgccggtgat ccaataaatc cggcaacaaa
    39181 catattcgct ggttggttat aaaccgtttt cggcgcacca acttgctgaa caatcccatc
    39241 cttcataatc acaatccgcg tcgccatggt catcgcttcg gtctgatcgt gggtcacgta
    39301 gatcatggtg gtattcagct tctggtgtag cttgctgatc tctgcgcgca tttgcacgcg
    39361 cagcttggca tcaaggttag agagcggttc atccattaaa aacacgcccg cttcgcgcac
    39421 aatcgcccgc ccgagcgcca cacgctggcg ttgcccgccg gaaagcgcgc ccggcttgcg
    39481 tttcaggtac tcacgcaggc cgagaatttg cgccgcccag ttcacccgct catcaatcac
    39541 ctctttggcg attttttgca tcttcaggcc aaacgccatg ttgtcgtaaa ccgtcatatg
    39601 cggatacaac gcgtagttct ggaacaccat cgctatattg cgtgttttgg ctggaacgtc
    39661 attcattcgt ttgccgtcga tcaacagatc gccgccgctg atctcctcaa gcccggcaat
    39721 catgcgcagg gtggtcgact taccgcagcc cgacgggccg acaaacacga tgaactcttt
    39781 atcggcaatt tccaggttga agtccttcac cacatgcacc tggttatcgt agattttttg
    39841 aatatgttgt aacgaaagct gagccatgtt gattcctttg ctatacgttt tgccagaagg
    39901 ccgataaccg cggccaagtg agtgattccg ttaaaggcaa cagtaattgc gccccggtta
    39961 agcccgcgcc gatccccacc gagcgcatac cgctggcgtt aatggcgtca atgcccgcct
    40021 gcgcatcttc aatgccgata catgcctgcg gcggcacgcc cagccctgca caggcggcga
    40081 gaaagatttc cgggtccggt ttcgagtttt taagttggga agcatccgcg cagaaggtga
    40141 aaaactcgcg cagctccagc gccgctaaaa tcgtcggcgc attcagggag acagaagcca
    40201 gcccaaccgg gatctgctgt gcacggagat ctgccagcaa agagcgaatg ccgggtagaa
    40261 cagcgttgac cgtcaactcg cgcagtgagt ggacatagag cagatttttg cgatacgcca
    40321 gttgcgccct ctcctgcgag ttaaagtcgc cctctttgcc cccgtgttgc agaatgcgcc
    40381 gcagagactc atcgcggctg atccctttta gggattcgtt aaactgcgca tcaatgctga
    40441 tgccaatttc agcggcaatc tgctgccacg cctggaaatg cagatgcgcg gtatcggtga
    40501 ttacaccatc cagatcgaaa attacccctt gcagtttcat tcatcctcct gatgtgtggt
    40561 agcggtccca ttgaagggca aaataaaatc acccaaacag aaaacagatt cttcagccac
    40621 ggatataagc tgaccgttca aacgcagtga aacgggcgca gaagttcgaa tcgcaatacg
    40681 ctgcgcgtca agagtgacct gtaattcgca gccctgccag aacagaggga aagacaactg
    40741 ttgccactgc tcaggtaacg ccggattgag atgcaattca ccgtcacgca cgctcacccc
    40801 gtgtaaccag gtgaatcatt tgataccgac aagggtttta gtgaaaggag gtgaaatcac
    40861 ctgtaagatg ctgaaataaa acaggtgatg tatacttcgg acggaaaact ctttttgata
    40921 ccgactgctc aacaaatagt caaaagatca aaaaacggcg acacatttga tactgctaat
    40981 ttacccttaa aatcttcttc aaaacatctt caaactgcca aaatgctcac ataatcgatc
    41041 taactataat cagtatgata atcatcatga attactattc aatagcgtga ttatcttggg
    41101 atgagtcata tgatctctat atgtatttac agcaccacct catgatagcg atggcgctta
    41161 accaacaaca tttttcttta ctgtcactgc ccctgattcc tgggtggcgt tattgctcga
    41221 gttgtacttc tcaataacgc gcaaaatctc tttgtctgtt tcttcacttc ccgccattcc
    41281 tatctttatt gcatttttta gtacgggacg taatggcaag gcatcaatga tgctttctac
    41341 cgcatcctgg ctgattgtag atgtattcat tgctgccagc ctgacaaggt tgttagtacc
    41401 ttctcgcctg atgaatgtaa ggattaattg cttctcttgg gggcttagag agctaagcgc
    41461 ctgatcaata cccgaacctg aatcgcaatt gttttcgatt tttccgcatt tatcttcgtt
    41521 cttgcctgtg gccaaccatt cgagtgaaca attacaggct ttagctatta aggccagttt
    41581 atccaaagga gggaagtttt tccccttcct gtaatttctt actgtggctt cagatagtcc
    41641 acaaagggtc gccagttcga cgtttgtcat gtttcccata gccatagcta agcgttctga
    41701 aaatcggttt attccttcca tatcaataaa ccgttccgca ctggatacgg ttttttctgc
    41761 cttatccata taacccccta taaaacaaag aaattatcga catcgcattt aatttcgata
    41821 tgctcgcaaa aaaaccgttg atatcgtatt attttgcgat tatgatttac ctaaatcgat
    41881 aaccggtttt gattatcggt tcaggtaaac atgagaggat cacacaatga gggtatccag
    41941 acaagcatcg aataatttgt ttgttcgaga ggggtgtgac tggtctgcta aagcggtata
    42001 tgcagccctt gaaaacaaag gcacagacct ccctgaactg gaaagggagt taggattaaa
    42061 aagcggatca atgcgcaatg tgttttaccg ccagtgccgt aaatacgaag ccgccatcgc
    42121 cgaaaaaata ggggttgaac ctgctgtaat ctggcccagc agataccagg tggacgccag
    42181 agaagtggct tgatggggga tgtatgtggg taacagcaaa agaatgtgtt ggtcttcctg
    42241 atttaccaat gatggaacat aacattcgcc atcgactcac aaaagcagct gcgggaaatg
    42301 cagccatgtt acgcaaacgt aaggggacaa aagcaatcga atttaatatc gattgtttgc
    42361 cttctggcgc gcaacaagta ttgcgtgaac gttatgccct tcaactgatg acgcaaaaag
    42421 ctgatgaatc accagctccg gtggtgacaa aggccagacg ctcgcctgcc gtggttgatg
    42481 cggtggaggc atatcgcgga tcaccacaac tgatggttga acgccttaat gccctgactg
    42541 aaaaccagcg ccaggtggct gatgcacgaa tcgcgatcgt cagcgaagtg atgaaagtcg
    42601 cgcaacaacc cggtttcagc tgtgcgaaag ctatccggtt tatcgttgac aacctggcac
    42661 gttcgcaact ggacgagcgc attgtggcaa tggttgagac ggcgaacgcc aaaaagggaa
    42721 acagccgtgc gttgagtgaa ataacgctga aacgctggat tgcggccttt aacaaggcgc
    42781 agaacgccgc tgaacgcctg cttttactgg caccgggtaa gcgccaggaa ataaaagccg
    42841 aagatattaa ctggctgccc gaatttctgg cgcagtatcg ccagtcaaac ggccgaccaa
    42901 tgaccgaggc ttacgaggat tttgtcgctg aatggcagca ccggcacgct gatgagcctt
    42961 atatgctcga tatcatgccg tcttatgaca ccattcgccg cgcaatgaag aaactgccgg
    43021 aagtggtgaa acaaaaaggc cgggtgaccg gcagtgaata ccgccagctt gagggattca
    43081 cgcgccgcga ctggtccaga atgccggtga attatgtctg gattggtgac ggtcacggca
    43141 tgaagctgaa atgcgcacac ccggttcacg gtcggccatt tgcaccggaa gtgacctttg
    43201 ttatcgacgg tggcacgcgc tttgtggtgg gctggagcct tgacctggct gaaaatgttt
    43261 tcgccgtagc cggtgccata cagcacggca ttcgccatca cggcaaaccg tttctgtatt
    43321 actcggataa cggctccggg gaaaccgccg acatcctgga taaggaggtt gtggggatac
    43381 tgccgcgact ggggattaat cacccgaccg ggattgccgg taatccgcag ggacggggca
    43441 ttatcgaacg gcttaaccgc acattaccga tgcgcatagc ccgtaaatac cgcacctatt
    43501 tcgggaaagg tgcagatcgc gagacgttac gcaaaaccaa ccgcgattta cgctcggcat
    43561 ttactgccct gcaacagggc aaacggctga acgcccggca gcagtcagcg atgcgtgatt
    43621 taccgtcctg gtctgaactg attgatgcca ttcgtgacgg tgttgagtgg tacaacaacc
    43681 ggccgcacga tgaattaccg gtgaagccga acggcaagca ttacagcccg gcggagttca
    43741 gaaaaaaacg cctggcggaa gaggacacgg aaattgaatg gctgtccgat gttgagctgc
    43801 gggatatgtt ccggccgatg gtggaacgcc ctgtaagacg ctgtgaaata cgctggctga
    43861 ataatattta ctacgcgccc gagctgcgtg acgagcatgg tcgtaaggtg cttatcagct
    43921 atgacattca tgatgccgaa cgaattaccg tacgtcgcct ggatggcagc gtgatttgcg
    43981 aggcggtatg ggacggtaat aaacgggaag ccttcccggt tagcgcggaa tactacaaac
    44041 agcagcaacg ccttaaaggt atgcgtaaac gcgcagagga aaaaatccgt gatgccgagg
    44101 atgaggttgt caacgtgctg gagcacaagc cgcaggagcc ctggctggaa aacatatacc
    44161 gccctgtggg taatacggtg accgttcagc aaccggtcgc tga
//
LOCUS       DAZKHC020000031        42119 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZKHC020000031 DAZKHC020000000
VERSION     DAZKHC020000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 42119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.031"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(122..1303)
                     /gene="cynX"
                     /locus_tag="ACHOSM_RS09910"
                     /old_locus_tag="pgaptmp_001982"
     CDS             complement(122..1303)
                     /gene="cynX"
                     /locus_tag="ACHOSM_RS09910"
                     /old_locus_tag="pgaptmp_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HGU4558516.1"
                     /translation="MRDHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSAQLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTITTPVRVVFTPRAWTLGLYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMVRHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHTLCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(1309..1779)
                     /gene="cynS"
                     /locus_tag="ACHOSM_RS09915"
                     /old_locus_tag="pgaptmp_001983"
     CDS             complement(1309..1779)
                     /gene="cynS"
                     /locus_tag="ACHOSM_RS09915"
                     /old_locus_tag="pgaptmp_001983"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HGU4558517.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(1808..2194)
                     /gene="cynT"
                     /locus_tag="ACHOSM_RS09920"
                     /old_locus_tag="pgaptmp_001984"
                     /pseudo
     CDS             complement(1808..2194)
                     /gene="cynT"
                     /locus_tag="ACHOSM_RS09920"
                     /old_locus_tag="pgaptmp_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
     gene            complement(2197..3186)
                     /locus_tag="ACHOSM_RS09925"
                     /old_locus_tag="pgaptmp_001985"
                     /pseudo
     CDS             complement(2197..3186)
                     /locus_tag="ACHOSM_RS09925"
                     /old_locus_tag="pgaptmp_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            complement(3226..4677)
                     /gene="prpD"
                     /locus_tag="ACHOSM_RS09930"
                     /old_locus_tag="pgaptmp_001986"
     CDS             complement(3226..4677)
                     /gene="prpD"
                     /locus_tag="ACHOSM_RS09930"
                     /old_locus_tag="pgaptmp_001986"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HGU4558518.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(4711..5880)
                     /gene="prpC"
                     /locus_tag="ACHOSM_RS09935"
                     /old_locus_tag="pgaptmp_001987"
     CDS             complement(4711..5880)
                     /gene="prpC"
                     /locus_tag="ACHOSM_RS09935"
                     /old_locus_tag="pgaptmp_001987"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HGU4558519.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(5925..6815)
                     /gene="prpB"
                     /locus_tag="ACHOSM_RS09940"
                     /old_locus_tag="pgaptmp_001988"
     CDS             complement(5925..6815)
                     /gene="prpB"
                     /locus_tag="ACHOSM_RS09940"
                     /old_locus_tag="pgaptmp_001988"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HGU4558520.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSARVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            7054..8640
                     /gene="prpR"
                     /locus_tag="ACHOSM_RS09945"
                     /old_locus_tag="pgaptmp_001989"
     CDS             7054..8640
                     /gene="prpR"
                     /locus_tag="ACHOSM_RS09945"
                     /old_locus_tag="pgaptmp_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HGU4558521.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLASERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYNVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGRFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(8738..9013)
                     /gene="yahO"
                     /locus_tag="ACHOSM_RS09950"
                     /old_locus_tag="pgaptmp_001990"
     CDS             complement(8738..9013)
                     /gene="yahO"
                     /locus_tag="ACHOSM_RS09950"
                     /old_locus_tag="pgaptmp_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HGU4558522.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            9160..9831
                     /gene="yahN"
                     /locus_tag="ACHOSM_RS09955"
                     /old_locus_tag="pgaptmp_001991"
     CDS             9160..9831
                     /gene="yahN"
                     /locus_tag="ACHOSM_RS09955"
                     /old_locus_tag="pgaptmp_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HGU4558523.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMCRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(9848..10093)
                     /gene="yahM"
                     /locus_tag="ACHOSM_RS09960"
                     /old_locus_tag="pgaptmp_001992"
     CDS             complement(9848..10093)
                     /gene="yahM"
                     /locus_tag="ACHOSM_RS09960"
                     /old_locus_tag="pgaptmp_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HGU4558524.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRGRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(10505..11299)
                     /gene="yahL"
                     /locus_tag="ACHOSM_RS09965"
                     /old_locus_tag="pgaptmp_001993"
     CDS             complement(10505..11299)
                     /gene="yahL"
                     /locus_tag="ACHOSM_RS09965"
                     /old_locus_tag="pgaptmp_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HGU4558525.1"
                     /translation="MISLKALHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKKYRDDARLVQELGDRYYPVNKHVKIRYTL"
     gene            complement(11542..12591)
                     /gene="yahK"
                     /locus_tag="ACHOSM_RS09970"
                     /old_locus_tag="pgaptmp_001994"
     CDS             complement(11542..12591)
                     /gene="yahK"
                     /locus_tag="ACHOSM_RS09970"
                     /old_locus_tag="pgaptmp_001994"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HGU4558526.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDLVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGLGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(12678..12752)
                     /locus_tag="ACHOSM_RS09975"
                     /old_locus_tag="pgaptmp_001995"
                     /pseudo
     CDS             complement(12678..12752)
                     /locus_tag="ACHOSM_RS09975"
                     /old_locus_tag="pgaptmp_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12968..14350)
                     /gene="yahJ"
                     /locus_tag="ACHOSM_RS09980"
                     /old_locus_tag="pgaptmp_001996"
     CDS             complement(12968..14350)
                     /gene="yahJ"
                     /locus_tag="ACHOSM_RS09980"
                     /old_locus_tag="pgaptmp_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGU4558527.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(14360..15310)
                     /gene="yahI"
                     /locus_tag="ACHOSM_RS09985"
                     /old_locus_tag="pgaptmp_001997"
     CDS             complement(14360..15310)
                     /gene="yahI"
                     /locus_tag="ACHOSM_RS09985"
                     /old_locus_tag="pgaptmp_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HGU4558528.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLAFLEQGGKEVIITTPECLPAALRGETGTHLIKT"
     gene            complement(15453..16871)
                     /gene="yahG"
                     /locus_tag="ACHOSM_RS09990"
                     /old_locus_tag="pgaptmp_001998"
     CDS             complement(15453..16871)
                     /gene="yahG"
                     /locus_tag="ACHOSM_RS09990"
                     /old_locus_tag="pgaptmp_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU4558529.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAADLAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(16871..18439)
                     /gene="fdrA"
                     /locus_tag="ACHOSM_RS09995"
                     /old_locus_tag="pgaptmp_001999"
     CDS             complement(16871..18439)
                     /gene="fdrA"
                     /locus_tag="ACHOSM_RS09995"
                     /old_locus_tag="pgaptmp_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU4558530.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLAEIEELFNSKAQSAKRKAQSG
                     SHEARYATIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDEL
                     ALKQLAHEKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHE
                     FGGGVSQLIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLE
                     RARACRKPVVVCFLGRVETPVDEQGLQFARGTKEAALKAVMLSGVKQENLDLHTLNQP
                     LIADVRARLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRS
                     IKHTFLDFGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPV
                     GSTIEAIKEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNT
                     GLLAREFICKGEEA"
     gene            complement(18429..19292)
                     /gene="yahE"
                     /locus_tag="ACHOSM_RS10000"
                     /old_locus_tag="pgaptmp_002000"
     CDS             complement(18429..19292)
                     /gene="yahE"
                     /locus_tag="ACHOSM_RS10000"
                     /old_locus_tag="pgaptmp_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU4558531.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMMGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEIIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(19332..19937)
                     /gene="yahD"
                     /locus_tag="ACHOSM_RS10005"
                     /old_locus_tag="pgaptmp_002001"
     CDS             complement(19332..19937)
                     /gene="yahD"
                     /locus_tag="ACHOSM_RS10005"
                     /old_locus_tag="pgaptmp_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HGU4558532.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            20195..20692
                     /gene="yahC"
                     /locus_tag="ACHOSM_RS10010"
                     /old_locus_tag="pgaptmp_002002"
     CDS             20195..20692
                     /gene="yahC"
                     /locus_tag="ACHOSM_RS10010"
                     /old_locus_tag="pgaptmp_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGU4558533.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            20784..21716
                     /gene="yahB"
                     /locus_tag="ACHOSM_RS10015"
                     /old_locus_tag="pgaptmp_002003"
     CDS             20784..21716
                     /gene="yahB"
                     /locus_tag="ACHOSM_RS10015"
                     /old_locus_tag="pgaptmp_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4558534.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(21758..22846)
                     /gene="pdeL"
                     /locus_tag="ACHOSM_RS10020"
                     /old_locus_tag="pgaptmp_002004"
     CDS             complement(21758..22846)
                     /gene="pdeL"
                     /locus_tag="ACHOSM_RS10020"
                     /old_locus_tag="pgaptmp_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4558535.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(22988..26971)
                     /gene="ehaA"
                     /locus_tag="ACHOSM_RS10025"
                     /old_locus_tag="pgaptmp_002005"
     CDS             complement(22988..26971)
                     /gene="ehaA"
                     /locus_tag="ACHOSM_RS10025"
                     /old_locus_tag="pgaptmp_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HGU4558536.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLAFGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAST
                     SDKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALIGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNAWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            complement(27544..29577)
                     /gene="betT"
                     /locus_tag="ACHOSM_RS10030"
                     /old_locus_tag="pgaptmp_002006"
     CDS             complement(27544..29577)
                     /gene="betT"
                     /locus_tag="ACHOSM_RS10030"
                     /old_locus_tag="pgaptmp_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HGU4558537.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            29706..30293
                     /gene="betI"
                     /locus_tag="ACHOSM_RS10035"
                     /old_locus_tag="pgaptmp_002007"
     CDS             29706..30293
                     /gene="betI"
                     /locus_tag="ACHOSM_RS10035"
                     /old_locus_tag="pgaptmp_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HGU4558538.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            30307..31779
                     /gene="betB"
                     /locus_tag="ACHOSM_RS10040"
                     /old_locus_tag="pgaptmp_002008"
     CDS             30307..31779
                     /gene="betB"
                     /locus_tag="ACHOSM_RS10040"
                     /old_locus_tag="pgaptmp_002008"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HGU4558539.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDVVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            31793..33463
                     /gene="betA"
                     /locus_tag="ACHOSM_RS10045"
                     /old_locus_tag="pgaptmp_002009"
     CDS             31793..33463
                     /gene="betA"
                     /locus_tag="ACHOSM_RS10045"
                     /old_locus_tag="pgaptmp_002009"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HGU4558540.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGESDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            33676..34344
                     /gene="ykgH"
                     /locus_tag="ACHOSM_RS10050"
                     /old_locus_tag="pgaptmp_002010"
     CDS             33676..34344
                     /gene="ykgH"
                     /locus_tag="ACHOSM_RS10050"
                     /old_locus_tag="pgaptmp_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HGU4558541.1"
                     /translation="MSEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYINK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQIPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(34587..35282)
                     /gene="ykgG"
                     /locus_tag="ACHOSM_RS10055"
                     /old_locus_tag="pgaptmp_002011"
     CDS             complement(34587..35282)
                     /gene="ykgG"
                     /locus_tag="ACHOSM_RS10055"
                     /old_locus_tag="pgaptmp_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HGU4558542.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(35275..36702)
                     /gene="ykgF"
                     /locus_tag="ACHOSM_RS10060"
                     /old_locus_tag="pgaptmp_002012"
     CDS             complement(35275..36702)
                     /gene="ykgF"
                     /locus_tag="ACHOSM_RS10060"
                     /old_locus_tag="pgaptmp_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HGU4558543.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHATSWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(36713..37432)
                     /gene="ykgE"
                     /locus_tag="ACHOSM_RS10065"
                     /old_locus_tag="pgaptmp_002013"
     CDS             complement(36713..37432)
                     /gene="ykgE"
                     /locus_tag="ACHOSM_RS10065"
                     /old_locus_tag="pgaptmp_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HGU4558544.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYINEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPMYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(37951..38805)
                     /gene="rclR"
                     /locus_tag="ACHOSM_RS10070"
                     /old_locus_tag="pgaptmp_002014"
     CDS             complement(37951..38805)
                     /gene="rclR"
                     /locus_tag="ACHOSM_RS10070"
                     /old_locus_tag="pgaptmp_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HGU4558545.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGMDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSRETLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            39031..40350
                     /gene="rclA"
                     /locus_tag="ACHOSM_RS10075"
                     /old_locus_tag="pgaptmp_002015"
                     /pseudo
     CDS             39031..40350
                     /gene="rclA"
                     /locus_tag="ACHOSM_RS10075"
                     /old_locus_tag="pgaptmp_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
     gene            40465..40701
                     /gene="rclB"
                     /locus_tag="ACHOSM_RS10080"
                     /old_locus_tag="pgaptmp_002016"
     CDS             40465..40701
                     /gene="rclB"
                     /locus_tag="ACHOSM_RS10080"
                     /old_locus_tag="pgaptmp_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HGU4558546.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            40713..41306
                     /gene="rclC"
                     /locus_tag="ACHOSM_RS10085"
                     /old_locus_tag="pgaptmp_002017"
     CDS             40713..41306
                     /gene="rclC"
                     /locus_tag="ACHOSM_RS10085"
                     /old_locus_tag="pgaptmp_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HGU4558547.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(41466..>42119)
                     /locus_tag="ACHOSM_RS10090"
                     /old_locus_tag="pgaptmp_002018"
     CDS             complement(41466..>42119)
                     /locus_tag="ACHOSM_RS10090"
                     /old_locus_tag="pgaptmp_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGU4558548.1"
                     /translation="LFITSKLWVQDMLNQDTAAAGIEASLKKSGLEYFDLYLLHQAMR
                     DYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMVNQVELHPYFAQPEA
                     LATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQVILRWNIQRGVVVIP
                     KSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNPEFVRGCLAVKIHD"
BASE COUNT        10283 a        11128 c        10243 g        10465 t
ORIGIN      
        1 agttgaatcg tcagtttaaa ttataaaaat tgcctgatgc gctgcgctta tcaggcctac
       61 aagttcagtg atctacgtta gccgcatccg gcatgaacaa agcgcaggaa caagcgtcgc
      121 atcatgcctc tttgacccat agctgcggaa aacgtgctgg tgcaaagcgc agggttatga
      181 tcatcagccc aacgacgcac agcgtatgaa atgcccagtc catcaggtaa ttgccgctga
      241 tactacgcag cacaccagaa aaccacgggg caagcccggc gatgataaaa ccgatcccct
      301 gcataaacgc caccagcttg ccagcaatag ccggttgcgc agagtgatcg agcgccagca
      361 gcaaacagag cggaaacgcg ccgcccagac ctaacccaca caccatcgcc cacaataccg
      421 gcaattgcag cggcaaccag ataaagccgc agaaacccac cagttgtaac accagcgcca
      481 gcattaacag tttgcgccga tcctgatggc gaaccatagc aggcatcagc aatgcccccg
      541 cagcttgccc aagcgtcatc aatgccagta aggaaccgct gtactgcgcg ctggcaccaa
      601 tctcaatata gaaggcgggt aaccaggcta tcaggctggc gtaaccaccg ttaatcagac
      661 cgaagtatag gcccagcgtc caggcgcggg gagtaaatac cacgcgaacc ggagtggtta
      721 ttgtcttgtg ggaagaggcg acctcgcggg cgctttgcca ccaccaggca aagagcgcaa
      781 caacggcagg cagcgcccac caggcgagtg tttgatacca ggtttcgcta tgttgaacta
      841 accagggcgt cagggcggca ccaagtccac cgccgcccat cagagccgcg gaccacagcc
      901 ccatcaccag tggcgtgcgc tgctgaaacc gccgtttaat caccgaaggc atcactgcct
      961 gaatgatgcc gatccccacc ccaccaagca gtgcgctgct aagcagctgc gcactttggg
     1021 ggtaaagctc acgcatcaat gcaccgacgg caatcagcaa cagactgatg gcgacactgc
     1081 gacgttcgct gacatgctga tgaagccagc ttccggccag cgccagcccg cccatggtga
     1141 ccaccggaag agcggtcaac agggcagcca cgctaaagct cattccgctc gcctggcgca
     1201 attgcggtag cagtggcccg acggaggtga gcagtggtcg catattaaga ccaatcagca
     1261 ccagaaccag cagcatcgtg aacgtctttt gatggtcgcg catggctgtc agaacggttt
     1321 ggtcggcaga tatttaccat ctaaggtgat gaccgcacgt tcgccacctt ccgggtccgc
     1381 cactttctta acgtcgagtt tgaagttaat cgcgctgata atgccatcgc caaacttctc
     1441 atgaaccaac gctttcaggg ttgtgccgta cacctgcaac atttcataga aacgatacat
     1501 cgttgggtcg gttggaatac ggtcatcaat gcagccacgc agtggaatca tctgcaacag
     1561 tagaatggcg tcttcgtcga gatccagctt cgccccgacc tggcgggcgg cgtcggcagg
     1621 aagcgcctgc tgacccagca aagccgcggt cacaaaggct tctgccagcc cggtgccgtc
     1681 ggcaatctcg gcaaatgaga gatctttttt agctttgctg agcaaaatgg catcggcaag
     1741 atcaagacga atattgcggt taatttgcga ctgaatcatg gtggatctcc tgataaatta
     1801 taaaaagtta cgctgcggtc ggttggcgta gcggtatggc acaaacgcga ggattagcgg
     1861 ccagtggcac aaactggcgg gttgcgccgt caaaagctgc gatgctgccg ctttcaatgt
     1921 cgtagaccca gccgtgcagg gcgatccgcc cctcttcgag cgccaggcgc accgatggat
     1981 gagtttgcaa attagccaac tgagcaatga cgttttcacg taccatcgca gcagcttttg
     2041 acggtaaatc gggatgcggg cgcgcctcat taacgacgcg ggctgaatcg gcataacgca
     2101 gccagtggga gacggcaggc atatggtcca tgcactgaca gctggcaatg gcggtcatcg
     2161 cgccacagtt ggaatgaccg caaatcacaa tgtcaacatc cggctaacct gatatttctc
     2221 gacgattttc caccacacgc cgcagtccgg ccaggtcggc aatccttcgt aaacgatagt
     2281 cgccatcccc gccagcagcg gcgcgtaaac gatatacgaa tgccccacca cccagccgat
     2341 atccgatgcg caaaagaaca cgctgcccgc tttgccgcca aaaatggtgt ccatcgaggt
     2401 cgccagcgcc accgcatatc cgccgacgtc acgctgcacg cctttaggtt tgccggtcgt
     2461 gccggaagtg tagagaatgc aggaggtttc gttggattcc agccacgcca ccggtacccg
     2521 cgcgccgatg tgttgatgac gcaacgacgc gaaatcgaca tcccgcccgc tgacgcgcgc
     2581 cattttcgcc agcccgcgat ccaccagcaa aacatggcgt ggctggtgct gcgcctgact
     2641 tatcgcatcg tcgagcaatt ttttataggg aatgattttg ccaccgcgcg ctccggcatc
     2701 agccgagaca atcagcaccg gtttagcgtc atcaattcgc gccgccacgc tgtgcgaggc
     2761 aaaaccacca aataccaccg aatgaatagc accaatgcgc gcgcaggcca gcagagtaat
     2821 atgcgcttcg gcaatcatcg gcatatacac cagcacccga tcgccgcgct gcacgcccag
     2881 tgagcgcaac attgaggcca ccgcgttcac ttcgtcatgc agctggcgaa aggtaaaggt
     2941 gcgctcttct tctgtttccg aagagacggc aatcaatgcc agtgcctctg gctgtttctc
     3001 cagccagcgg tcgatagcgt tgtggcacaa attggttcgg ccttcacaaa accaacgggc
     3061 aaacggcgga ttgctgtggt cgagcgtttg cgtaaagggc gtctgccagt caatacgccg
     3121 ggcctgctcg gcccagaact gctccggttc gttaatcgaa cgctgataaa attcgctaaa
     3181 agacataatg ctctcctgtt gaacttacgc cttaccgccg tttacttaaa tgacgtacag
     3241 gtcgagatac tcattgaccg gcatctgttc caggcgagtt ctgtcgagag aaacctccag
     3301 aatgcgctgc tgttggcgag tcgggaactg gcgcgcgaga ttgattttga atttatcgac
     3361 cagtttcgga ataccatcct gacggcggcg agcatgacca atggggtact ccaccaccac
     3421 ttcttcaaat cgtgtgccgt cggtgaactc aagggtaatg gcattggcga tggcgcgttt
     3481 ttccgggtcg tggtagtcag cggtaaatgc cggatcttca aagcaattga tcttctcgcg
     3541 cagggcgtca atgcgtttat cttgcgcaac gttgtcctcg taatctgccg ccgttaagcg
     3601 cccgaatagc agcgggatcg ccaccatgta ctgaatgcag tgatcgcggt ctgccgggtt
     3661 attgagcggc ccttttttgt cgatgatgcg aatacaggct tcgtgggtgc gaatggtcac
     3721 tttttcgata tccgccgccg ttttgcctgc tgcctgcatc tgttcataga gcgtcatcgc
     3781 tgcttcaact gccgtctggg agtggaactc cgccgggaag gagattttga acagcacatt
     3841 ttccataacg taggaaccgt acgggcgctg gaagcggaac gattcacctt taaaggagac
     3901 gtcgtagaag ccccacaccg gcgcagtcag ggctgacggg taacccattt cgcccgtttt
     3961 cgccatcagt gccagacgta ccgcgcggga agtggcatcg cccgccgccc aggatttacg
     4021 cgtgccggtg ttcggcgcat ggcgataggt gcgcagcgac tgaccgtcca cccacgccag
     4081 cgaaacggcg ttgagaattt cctcgcgggt caggccgagc atttcggcga ccacggcggt
     4141 ggaagccact ttcactaaca gaacgtggtc gaggccgacg cggttaaagg agttttccag
     4201 cgcgatgcag ccctgaattt catgggcttt gatcattgcg gtcagcacct gtttcatggt
     4261 caacggcgct ttgccgctgg cgaccgcgtt gcgcgaaagc cagtctgccg ttgccagaat
     4321 gccgccgagg ttgtcggaag gatgccccca ctccgccgcc agccaggtat cgttgaaatc
     4381 gagccagcgg atcatcgcgc caatgttaaa tgccgcctgg acggggtcga gctgaaactg
     4441 agttccgggg acgcgcacgc cgttgggtac gacggtgcca ggaacaattg gccccagcag
     4501 ttttttacag gccgggtatt cgagagcttc aagaccgcag ccgagcgtgt cgagcaggca
     4561 gtaatgtgcg gtgtcgtagg ccactttaga gctgatttcg tagttcatca cgtaatcgac
     4621 gatatcaacg atttcacgat caaattccgg gcggatgttg ttgatttgag ctgacattgg
     4681 gtacgtttcc ttattgttat tcgtagaggt ttactggcgc ttatccagcg cgacaaactg
     4741 gcggtcttcc ggtccaacat aattggcgga aggacggata attttgttgt cctgacgttg
     4801 ttcaatgatg tgcgccgccc agccggtgac acgggcgatg acaaacagtg gtgtgaacat
     4861 ctcggtggga acgcccatca tgttgtagga aacagcagag aaccagtcga gattggggaa
     4921 catctttttg ctctcccaca tcaccgtttc caggcgatcg gcgatgttgt acatcttcaa
     4981 cgagccgcct tcctgcgaga gctgcttcgc cacacgtttg atcacctggt ggcgcgggtc
     5041 ggcgatggta taaaccggat gaccaaaacc aatgaccact tctttgtttt ccacccgctt
     5101 gcggatatcg gcttcggctt cgtccggcgt ttcgtagcgt tgctggatct ccagcgacac
     5161 ttcattcgct ccgccgtgtt tcggcccgcg cagtgcgcca atcgcgccaa taatcgcgga
     5221 atacatatca gagccagtgc ccgcaatcac ccgactggta aaggtggagg cgttaaactc
     5281 gtgttcggcg tacagcacta gcgaaatatg catcgccttt tcccagcttt gcgatggctt
     5341 ttcgccgtgc agcagatgca ggaagtgacc accgatggag tcgtcatcgg tttccggctg
     5401 gatgcgttcg ccgttgtgac tgtagtgata ccaataaagg agaatcgagc taagtgatgc
     5461 cagcagtttg tcggcaatat cccgcgcgcc agagacagtg tgcccctctt tttctggcag
     5521 tgtgcagccg agcgcggaaa cgccggtgcg catgacatcc atcgggtgcg acgccgccgg
     5581 taaggcttcc agcacggtac gcacgttagc cggtaaacca cgcagggctt tcagtttcgt
     5641 tttgtaagcg gcgagttcgt cacgggtcgg cagtttgccg tggatcagca gatgagccac
     5701 ttcttcaaat tcgcaatgtt ccgccagatc aagaatatcg tagccacggt aatgcaggtc
     5761 attgccactt ttacccacgg tgcagagcgc cgtattgccc gccggaacgc cggaaagtgc
     5821 caccgatttt ttcggtttaa tgacatgggt actgttttgc aggatcgttg tgtcgctcat
     5881 attgtcctcg tcatttgtag ggtcgagata tttttcgttg tgttttattt cacctggcta
     5941 cgggcaaaca ggtcgtcgag cttctcttcg tactggtagt agttgatgct ttcgtacagc
     6001 tcgttgcggg tctgcatggt gtcgatgacg cttttctgcg tcccttcctg acgcaggacg
     6061 ttgtagacat gttcagcggc gcggttcatg gcgcggaacg ctgaaagcgg gtacagcgcc
     6121 attgcgacac gggcactgcg caattcatcg gtggtaaaca gcggtgttgc gccaaattcg
     6181 gtaatgttgg cgaggatcgg cacctgcacc gcatcggcaa actggcggta catggcgagt
     6241 tcggtaatcg cctccgggaa caacatctcg gcacccgctt caacataggc ctgtgcacgc
     6301 tcgatcgccg catccagccc ctctaccgcc agcgcatcgg tgcgcgccat gatcacaaaa
     6361 tcaggatcgg ttttcgcatc caccgccgcg cggatccgat ccaccatctc ctctttcgag
     6421 acgatcgctt tgttcgggcg atgaccgcag cgtttcgcac caacctgatc ttcaatatgc
     6481 aatcctgccg caccggcttt aatcatcgat ttcaccgtac gcgccacgtt aaaggccgaa
     6541 gaaccaaaac cgatatccgc atccaccagc agcggcagcg aacaaacgtc ggtgatacgg
     6601 cgaatgtcgg tcagcacatc atcaagggta gaaataccga gatcgggcag ccccagcgaa
     6661 cctgccgcca cgccaccgcc ggagagataa attgcctgat atccggcacg ctgcgccaac
     6721 agcgcatgat tagcgttgat ggtgccaaca atctgcaatg gattttcttt agacagtgca
     6781 gcgcgaaacg ctttacctgg agagtgtaga gacatagccc atcctttgtt atcaacttgt
     6841 tatttgcgtt gataaagaca aagcaagggg tgtgccacaa ttgctttaat aaaattaaat
     6901 ctttaatttc aatttgttat aaataaagtc atgaactaat ccgcaaatat gcgtttcagt
     6961 taacgtttca ggcaatgttt caccgcgttt cattgcaaca attatgaaac aagactaaac
     7021 ccaatattcg gtttcttaac tttgcggtgc gctatggcac atccaccacg gcttaatgac
     7081 gacaaaccgg ttatctggac ggtatctgta acgcgcctgt tcgagctgtt tcgcgatatc
     7141 agcctcgagt ttgatcacct ggcgaatatc acccctattc agcttggctt tgaaaaagcg
     7201 gtgacctaca tccgcaagaa actggcaagc gaacgctgtg acgccatcat cgcggctggc
     7261 tctaacggcg cgtacctgaa aagccgcctg tcagtgccag ttattttgat taaaccgagc
     7321 ggctacaatg tgttacaggc actggcaaaa gccggaaaac tcacctcttc tatcggcgtt
     7381 gtcacctatc aggaaaccat tccagcgctg gtggcgtttc aaaaaacctt taatctgcgc
     7441 ctcgatcaac gtagctacat taccgaagaa gacgcgcgcg ggcagattaa tgagctgaaa
     7501 gctaacggca ccgaagcggt ggtcggcgcg gggctgatta ccgatctggc agaagaagcc
     7561 ggaatgactg gaatttttat ctattccgcc gccaccgtgc gccaggcgtt cagcgatgcg
     7621 ctggatatga cgcgcatgtc gttacgccat aacactcacg atgctacccg taacgccctg
     7681 cgtactcgtt acgtgctagg cgatatgctc ggtcaatcac cacagatgga acaggtgcgg
     7741 cagactattt tgctgtatgc ccgctccagt gcggcggtgt tgattgaggg ggaaacgggg
     7801 acgggcaaag agctggcggc ccaggcgatt catcgggaat attttgcccg ccacgatgcg
     7861 cgacagggca aaaagtcgca tccgtttgtt gccgtcaact gcggggcgat tgccgaatcg
     7921 ctgctggaag cggagctgtt tggctatgag gaaggggcgt ttaccggctc gcgacgcgga
     7981 ggtcgcgccg ggctgttcga aattgcccac ggcggtacgc tgtttctgga tgagattggc
     8041 gaaatgccgc tacctttgca gactcgcctg ttacgggtgc tggaagaaaa agaggtcacc
     8101 cgcgtcggcg ggcatcagcc tgttccggta gatgtacggg tcattagcgc cactcactgc
     8161 aatctggaag aagatatgcg gcaaggacgt tttcgccgtg atctgtttta tcggctgagt
     8221 attttgcgtc tgcaattgcc accactgcgc gagcgggtgg cggatattct gccgctggcg
     8281 gaaagctttt taaaagtgtc tctggcggcg ctctccgccc cgttttctgc cgcattacgc
     8341 cagggattac aggcaagcga aaccgtgctg gtgcactacg actggccggg caatattcgt
     8401 gaactgcgca atatgatgga gcgactggcg ctatttttaa gtgtggaacc gacgccggat
     8461 ttaacgccgc aatttttgca gctgctactg ccggaactgg cgcgcgagtc ggcgaaaact
     8521 cccgctccac gcttactgac accacaacag gcactggaga aatttaatgg cgataaaaca
     8581 gcagcggcaa attatttagg catcagccgg acgacgttct ggcggcggct gaaaagctga
     8641 actgccgaat gcagcgcgaa cgctttatcc ggcataaatt tcaatagatg atcaccgata
     8701 aacttacgca tcggagatgt ttacatagga ttcagaatta cttcttctta taaatatttg
     8761 ccgtgccgtg gatcttattg tcagtttgac cggaggtcag taccaacaca tcagcccctt
     8821 tttcatccgc ttttttgatc aaatcttctt ttgcatctgc agtcgacatt tcattgctgg
     8881 ttgaaatatc acctattttt tcatactgcg attcaacttt ttcaaattcc gctttggtca
     8941 tcaattcagc ggcatagaca ttggtaacgg ctaacgctaa cgcaccgact aacattttag
     9001 agattatttt catattacgg ctccagagtt gttatcacac tatcacccac ccacattatt
     9061 gggttgatta ataattagtt cagtcagggc ataaacgcca gtaaccggaa ataagacact
     9121 ttgtgatctc tctcgcaccc gattattttt ggtagaaata tgatgaagtt acttcactta
     9181 tttatggatg aaatcactat ggatcctttg cacgccgttt acctgaccgt aggactgttc
     9241 gtgattactt tttttaatcc gggagccaat ctctttgtgg tagtacaaac cagcctggct
     9301 tccggtcgac gcgcaggagt gctgaccggg ctgggcgtgg cgctgggcga tgcattttat
     9361 tccgggttgg gtttgtttgg tcttgcaacg ctaattacgc agtgtgagga gattttttcg
     9421 cttatcagaa tcgtcggcgg cgcttatctc ttatggtttg cgtggtgcag catgtgccgc
     9481 cagtcaacac cgcaaatgag cacactacaa caaccgatta gcgccccctg gtatgtcttt
     9541 tttcgccgcg gattaattac cgatctctct aacccgcaaa ccgttttatt ttttattagt
     9601 attttctcag taacattaaa tgccgaaaca ccaacatggg cacgtttaat ggcctgggcg
     9661 gggattgtgc tcgcatcaat tatctggcga gtttttctta gtcaggcgtt ttctttgccc
     9721 gctgtgcgtc gtgcttatgg gcgtatgcaa cgcgttgcca gtcgggttat tggtgcaatt
     9781 attggtgtat tcgcgctacg cctgatttac gaaggggtga cgcagcggtg agcagataat
     9841 ttactctcta cgtaatcaac ctgatttgcc gaatcccggc catcggcgat gatagccccc
     9901 gtaatgcgtg gtccttattt ccgataagtt tacttgagtc tttagcttcc catttcagcc
     9961 cactctcccc tgtgtgggct tttttcattg gtctgccccg tcctgaatag ccaaacaatg
    10021 gcaatgttcc actttgcagg gatgataata aggggatctg attcagaaga gttttgaaaa
    10081 gttggaccgc catcgaggcc tcgaaccccg taccatacaa caatcctggt tgtcgtctgc
    10141 tcttcctgat gagctaatgg cggtttttga tatgagcctt acagcatatt ttctgtcagg
    10201 cttgagtgcg atgcgtaaga tacagaaaag accccacaca aacatgatgg aattatgatt
    10261 tttttcatat gaatgtcaac ccacacattc acaaaacatc tgcatgagat cattttttat
    10321 acaccgcatg gctgcgaaac cactgctggt gcttttttaa gcgaagtgtc gccgatcaca
    10381 acaaaatatc ggcgaaaagg cggtggaatg ccatttggca tacggatata cagatgaaat
    10441 gattacctgc tttgcgattc actctctggt ggaataacaa cttgcaggtg gaaccctttc
    10501 tgaactaaag agtgtagcgg attttaacgt gcttatttac aggataatat ctgtcaccta
    10561 attcctgcac taatcgtgca tcatctctgt atttttttac gtccagaaca gagagcaata
    10621 acatcaccag tgattcagga aaaacagggt agatattttt taagtgtaat aataatccat
    10681 gcgcaagcgc ctgcttaatg ggcgcgtctg tctccggaat agacgacatt tcgcgaagcc
    10741 atttttccac atgcatacac tctttccctt tagggaaaag tctcagcaaa ggcatatggt
    10801 gttcaagaag aatacatttg caatctgttc gcactgaatt gataaactgc actaatttct
    10861 taagcccggt aaagcgtcca gcctgcacat ttgctttaat atcaagaaac gcatcatcct
    10921 ggtcatatac ccaaactgtc cctttttgat tttcaagtat gcgaaaaaca aacttaaccg
    10981 ggtgatcagg gtaatccatc cagtcataaa atcctgggaa atcattaaac tgcacattta
    11041 tggtgagtgc gttatcgtct gaccactcac agtctcgtac ggcagccatg agatgaatga
    11101 gtttgtcgtc atcgactaaa tcatcacgac aatcataagg ataatcataa tattgtttaa
    11161 tcaaaacctt gaatatatcg acacaggaaa ccagggagct tttgatatcc tctggtaact
    11221 gattgttttt aaccgtatta agtatgcttt gttgcgtata tggcatcaaa ttattgtgca
    11281 gagcttttaa tgatatcatc gcgatctctc ttaaataaaa ataaacgtcg cagataataa
    11341 agcgctgatt aaaccaggca ctatcagaaa tcgctcataa ctaacgtgtg aagtattgtg
    11401 tactggaggg agttaattta acaatttgaa tttacgttat aggttttact gctactttga
    11461 tattatttcg ctctccttaa ctaaaaagtt aagtggagag cgaagttaat atgttaaacc
    11521 acagcgtatt tattaatttt ttcagtctgt tagtgtgcga ttatcgataa caaaacgata
    11581 tttcacatca ccacgcagca ttcgctcata ggcttcatta atttgatcgg cccgaatcat
    11641 ctctatatca gccacgatgc catgttcggc gcaaaaatcg agcatctcct gagtttctgg
    11701 aatgccgcca atcatagaac cggctatcgc acggcgtttc atgatcaggt tgaaaacttc
    11761 cggcgattta tgcggtgtcg caggcgcacc aaccagcgtc atggtgccat cacgcttcag
    11821 caaggtggta aaatcgtcga gattatgtgg cgcagctact gtattcaaaa tgaaatcgaa
    11881 actcttcaga tgagccgcca tctcatcggc attgcgtgag ttaacaactt catcggcccc
    11941 cagggctttt gccgcttcgc gttttgcctc agaagtggta aatgccacca catgcgcccc
    12001 catcgcgtgg gccagcttga tccccatatg tcccagaccg ccgatgccga ccacgcccac
    12061 ttttttaccc agcccggcct gccagtgacg tagcggcgaa tacgtggtga tccctgcaca
    12121 caacaaagga gccaccgccg ccagctgctc ttgcgggtga cgaatacgca gaacatatcg
    12181 ctcatgaacg acgatctgtt gtgagtagcc gcccagagta tggcccggtt cgtccggcgt
    12241 cggcgagtta taggtgccgg tcatgtgatc acagtagttt tccagcccgt cttcacactc
    12301 ttcgcaatgt ttacaactgt cgacaatgca gccgacaccg accagatcgc ccggcgcatg
    12361 tttttctacc agatcaccaa cggctaccac acgccccaca atttcatgac ccggtacgca
    12421 ggggtaaacc gtccccgccc actcggaacg gacctggtgg agatcggaat ggcagacgcc
    12481 acagtaagcg atttcgattt tgacatcatt cggtcccggt tcacgccgtg tgatatccat
    12541 cggttcaagt ggttgtttag cggaatatgc accaacagct ttgatcttca ttgtgtttac
    12601 tcctgattag ctatgtgtat ttggcgatgc ctgatctgtc atcgcggcaa aacgcgtcca
    12661 ttgaatatag ccaatattta aaccacccga tccagcccca acatgccgcg aacctgaatg
    12721 aaacagagca gcaacgttcc ccataaggcg agcgcctata agtgtgatat ttgctatccg
    12781 taaaagacta aaatcggcaa aactgatgat cttttcacgt agacaaatag ttatctacac
    12841 aacgtcaata agggcatatt cctcaaagat aatgtccatt tctgccgaca ctatcatatt
    12901 gaggtcgccc cactccctgg ggcttaaatc tcttacaatt caccgttgca ggcgtttcaa
    12961 atgcgcatca acctgccaca ctcccccaca ccagttgccc tttatggaac gttgcggttc
    13021 tcggcgagat acgcgccacc gcctcggcgg aacaggaggc gtccaccagc acaaagctgg
    13081 catcatcctg cgcttttggc catacacgct cgcctttttc attcagcggc aatacatcgc
    13141 cagtggctag aaacagcgaa cgggagaggt tctgttcgtt aggacgaata tagagctgcg
    13201 cgtagagatt ggctttttcc agcatgtcgc ccagaccata aggcgaccag tggtcgataa
    13261 cgctgtcagt gccagtcatc acttttacgc ctttgtcgtg caactgtttg agcggcatat
    13321 gcagcgtgcc aatcggcacc gtcgaggcga tagaaatttg ctgcgccgcc atccgattcg
    13381 ccagcgcatc gacctgttgt tcgttgagcg tagccagcgc aaaggcgtgg ctgatggtca
    13441 gcttaccttt cagttgcggc gttttctcta ccgtttcaac catataattg atggctgcca
    13501 cgcccgacgg agtggtttcg tgcaggtgaa tatcgacacc tttgtcgtag tccagcgcaa
    13561 tctgaaacat ggtgtcgagg gatttttcca tcgcgccatc aacactggtc gggtccagcc
    13621 cgccgacgta atgcgcaccc gcctgcatcg cttcgcgcat taagggttcc gatttcgaca
    13681 gcagcaaacc gtgctgcggg aaggcgacga tttcacactc gaagcccgcc tgacgtcgcg
    13741 ccagcaccgc ctgcaaattc tgcagatttt tcaggccgga aaccggttcg atattgcagt
    13801 ggctgcgggc aatggtggtg cctttcgact gcaatagatc gatcagtttt tctgcccgtt
    13861 cctgcgtgta cggttgcagt tccggcagca ttttctgctc aagcttgatc atgtcctgga
    13921 tggtggtgcc tgccggacga ttgagcgagc gccacggccc gccgtagaag gttttgtcga
    13981 gatgaatatg catatcgcga gtggtgggca gcatcagctt accgccagca tcatagtgcg
    14041 gcagcgtggc gtccggatgc tgcttgttct cgcgcagggc aacaattttg ccgttttgaa
    14101 tctccacggt ctggcgcgcc gtgcgggtct gtaccgccac accgttttcg tagtcaaaac
    14161 cggtttccag tagcacgttg tcgagatagt aatgcgggtc agtgattttc atggtgttat
    14221 tcgcgtcgca gtgggttgtg ccagagactg ccgcatgggc gagcggcaca gaggtaccaa
    14281 acagcgcggc agcggtgacc atcttaccgc tctggctcag aaattcacgg cggctattgt
    14341 tttctttcat cttacgtcct tacgttttaa taaggtgggt gcccgtttca ccacgcagcg
    14401 ccgcaggcag gcattccggc gtggtgataa tcacttcttt gccgccctgt tccaggaacg
    14461 ccaggctggc gatgattttt ggcaacatgc tgcccggcgg gaaatgccct tcctgcatat
    14521 agcgggtcat ggtggcaata tccacccgat ccagcgcctg ctgctgcggt ttgccaaagt
    14581 ggatacagac tttttcgacg ccggtggtga tcaccagaat gtcggcgtga atttcacggg
    14641 ccagcagcgc ggtagagaga tctttgtcga taaccgcgtc cacgctttgg taatcccccg
    14701 cttcagtacg cactaccgga attccgccgc cgcccgcacc aattacgaca aaaccttgtt
    14761 ggatcagcgc cttaatggca ggtgcttcga caatacgttt cggttccggc gaggcgacca
    14821 cgcggcgata gccccgtccg gcatcttcaa caaaacacca gtcagggttt gccttttgta
    14881 gttcgtcacg ctggctttca ctgaagaatg caccaatggg cttggtggga tgggcaaaac
    14941 ctggatcgtt tttatccact tccacctgag ttaccacggt gacggctttt ttctcgccgt
    15001 gacgcgccag ccggttattc agcgcctgtt ggatcagata gccgatgccg ccctgcgtat
    15061 ctgccacaca gttcgccagc ggcgttaagg gcagcccttc gcgctcgtgg gcaatctccg
    15121 cacggcgtaa atccagcccg acctgcggcc cgttaccgtg ggtcagcaca atgtcgtaat
    15181 cggaagccag catttccagc accgtatcgg ccacggcttt caccgcctcc gcctgatgct
    15241 caatcgactg gctggcgtta tctttgataa tgctgttgcc accaatggca acgaccacaa
    15301 gctctttcat gttgttatgt ccgctacaga gggtgatggg gttatgacca acgccagact
    15361 gcctgatgcg ctacgcttat caggcctaca attctcgggc catccgtagg ccggataagg
    15421 cgttcacgcc gcatccggca tgaacatgta ggtcagacgc cgtaacgttc gcaccagcca
    15481 agaatggctt tctcgaagca ggcaaaaggt ggatgcacaa tgcccgcgcc aatcatgccg
    15541 acgcccgcat ctttatgggc gatagcagtg ttgatcaccg gcagaatgcc gctgctaccc
    15601 acgcgggtga tgtcgattgc cgacggcacg cccataaaac cgagcagcgg aatggtgacg
    15661 ttggggtttt caccgagagt gatttcacgc atctggcgag agaaatcgat agcctcttcc
    15721 accgtgccgc ccaccagcgc aacgattgca ggcgcggtcg ccatagcaaa tccgccaata
    15781 ccgtaggttt cagtgatggc gctgtcgccg atatccagcc ccgaatcttc cggcttataa
    15841 ccggcaaaca tcgggccgat gacctgctgc gccggaccgg taaaccattg ccccggtaat
    15901 ccgctgaccc gcaggccgaa ctcgacgccg ttacgcgcca tggtggtgac cacggtgctg
    15961 tattcgatgc cgtgcgccgc atccatcgcc gctttacaca tcgccatcca cgtcgggccg
    16021 gagaagtagt cgctgctggc gacaaactca aacacttcgc gctgtttctc cacggaataa
    16081 cctgcctgaa taatccccgg cgtcagcgcc tgaatcagca acgtcgtccc ggcgttattg
    16141 cggttatggc actcatcgcc catatgcagc gcctgcgcca gcattaagcg cagatcgatc
    16201 tcaccgataa ttttcatcgc gtcgcgcagc attggcccct gcacatcgcg catccagttc
    16261 aggcggtcga tcacgctctg atcgttggca cccatacgca aaatcttcgc catctgctcg
    16321 ctcatattgg tgtaagcgat gttgccgtag gttttgtttt tcacgatgtg cataaacatc
    16381 gaggccgagg tgacgcccgc catcgatccc acacagtcgt gttcgtggca cggcgagaag
    16441 gtgatctccc cggaggccgc caggtcagcc gcctcgtcga gatctttcgc cagtccttcg
    16501 aacaccagcg ctccggtgac cgcgcctttc atcgcgccgc acattttttc ccaggtgatc
    16561 ggcggcccgg cgtgaagaat ggttttcggc gtcatgcccg gcaccacgtt aatcgcctga
    16621 tcaaaaccaa tcagcaccgg atgcgactgg ataatgcgct ccagcgcctg ctggttagcg
    16681 gcggcgattt tgtccgccag cggcgaatcg gcaatgttat ccagcgcctg caccacctgc
    16741 atattgccct gccccggcgg cgtccagtcg agttgggtca cttctacatg ctgctttttc
    16801 aggtcatcgc taaacatggc gatgccgacg ttaataacgt tcaatggttg gctaaacagt
    16861 gactggctca tcaggcttcc tcccctttgc agataaattc acgcgccagc aatccggtat
    16921 tggtgctgct gctcgccaga atcactccgg catcaagcaa catctggctt tgttgttcta
    16981 atgatggcgt atcgagatcg gtgcccagca cataggcgag aatgatcaac tcgcgtcctt
    17041 cggcggcggc gatcgctttc gcttctttga tcgcctcgat ggtggagccg accggatctt
    17101 catgcgatcc aaatccgagc acaaaatcca tcacgatcac cgccacttct ggatcgcgcg
    17161 cttcttcgat caagcgactg atgcggttgg tggggtcaat cattggatgc ggcttgccgt
    17221 tggtgaagtc gtcatcgcca aagtcgagga aggtgtgttt gatgctgcgg ttgatatctt
    17281 tcaggcggaa ttccggatcg ggctgaatgt tgctgtagac atcgccatgt ttttccatca
    17341 ccgcgaacat ggtttcgtcg cacaacgtgc cgccgcagaa caggccacga atgtatttct
    17401 gctgcggttg cagacgcgca cgcacatccg caatcaacgg ctggttaagc gtatgcaggt
    17461 cgagattttc ctgtttcacg ccggagagca tcaccgcttt tagcgccgcc tctttggtgc
    17521 cgcgggcaaa ctgtagcccc tgctcatcca ccggcgtttc aacgcgaccg aggaagcaga
    17581 ccaccaccgg cttgcggcag gcacgtgcgc gttccagcac tttgcgggcc accgcaggtg
    17641 cgggaggttt tgaaatcagc gcgataattt cagtttgcgg atcgttttcc agcatcccga
    17701 tggcgtcgag catcatcagg ccgccgattt tctcgctcag gtcgcgcccg ccggtgccaa
    17761 tcagttgtga aacgccgcca ccaaattcat gaatgcggac gctcagctcc tggctgccgg
    17821 tgccggatgc gccaacaata ccgatgttgc cgcgacgcac ggcgttacca aaacagagcg
    17881 ccgcgccgtt gataatcgcc gtgccacagt ctggccccat catcagcagc cctttttcgt
    17941 gggccagttg cttgagcgcc agttcatctt caactgagac gttatcggaa aacagcatca
    18001 cgttgagatc gttttgcagc gcctgacgcg cttcgcgagc ggcaaacaga ccgttgaccg
    18061 aaatcaccgc caggttactt tccgggatat gctttttggc gctggcaata gtggcgtaac
    18121 gcgcctcgtg cgagccgctt tgcgctttgc gctttgcgct ttgcgctttg ctattgaaca
    18181 gttcttcaat ctccgccagc aactgctcat tgtccgcacc cgatttacca ttgatgacaa
    18241 tcatcaggtc gccgtttttc gcctgttcca gctccggcgt cagcagtcct aaatttttca
    18301 gcacaccttt gttcatttcg gtcgccatcg ccacaaatgc ctgctcgacg ccgtcgagtt
    18361 tatttgcacg cgtggagata gacatcagcg agacagaatc aaaataggta ttcggtttaa
    18421 tgactatttt aactgacata attccctccg taggtttggc tcagcgcaca accatcggcc
    18481 atgccataca gcatgtcgca gccggaactg gagccaatat gtttaattat ttctattgcc
    18541 tgagttgaat tcccagggac gccataaata atgttgtgaa taaagcgatg aatattttcc
    18601 cggcagcgtt gttgtaatgc ggcttccagc gttatggcac ttaataacgt ggtgttattt
    18661 ctgccgcgta gcagaccgcg ataaaattcc tctttgtatt tttctgccgg atgcccggga
    18721 ataaataaaa taagcgccag acctgttaaa taatcatcgg ctgatggcgt aaggccaatc
    18781 cctaacccca tcatactggc gaccgctgcg gcgatatttt gcttatcgtt tactgtctga
    18841 ataactgccc gtcgtttaat atgtaattct ttatttaacg cctgataaaa cggattatcg
    18901 ccgcgataat taaacagggt ttcattttcc cccaggtgct gatgaataat gtcgtgccac
    18961 tgtagccagg cgataaggtg aaaattcaca gcggtcattt gcaaggttgg ggacagccaa
    19021 cgccgacaac gactcatctc tatatgaaga tgttgaccaa ccgtaatacc ttgatcgtca
    19081 aactgaacct tatcaccatg ccggaacaga tcatcaaagt gagtgagtgc caaccgacaa
    19141 ctgtttggcg cattgtcgta ctcttcacaa agcagcgtca gcaactgctg gcgagccggg
    19201 agtgcgatat ttaccgctct ggcaaagacc tgttcaacct gtccttgccc ccgctgcgcc
    19261 agaaaatccg catccgcagt tagcgcccac acacaccgtc tttctggtgt gttgatagca
    19321 agcctcccgg tttatgcacc tgcggcaatc agtaactgcg caatctcttc aaagccccgt
    19381 tcccgcgcca gttccagtgg cgttttgcca tatttatcgg tcagatgcgg gctggcaccg
    19441 tgttccagca ataactgcac aatcgcctgc tgtttaatac caccatcatt aagcacaatc
    19501 gcttccagta gcggcgtcca gcctacatgg ttggtctggt taacgttaat ctccgtatgc
    19561 gccaaaagct cttttacaat acttaaatgg cctttttcac aggcaggcgt caggccgaca
    19621 ccgccaaaac gggttacgca attaagatcg ggtttagccg gtaaaataat tcgtagtagc
    19681 gttaaatcat cgttcagaca gctaattaaa aaaggattta aacaggtatg atcttgctta
    19741 ttaatatccg ctccggcatc aattaatgcc tgaacgcaag catattgctg atataaactt
    19801 gccagcgtaa ttgccgtttt cccctgacga tcgcaggtat taatatcgac acccagcgca
    19861 aggcaggttt ttactttatc gatatcaccc tgttgtgcag ccaataaata gtcggcaggt
    19921 agatttttta tactcattca cattcccctg gtttttgtgg ctgttttcga tgaattgaga
    19981 atagcaacga taatctgata aaagaacccg cttaaattta attcgccgta tctcgccttt
    20041 ttgttgaata atattcaaca atcttttcgc agtgtgcggt agtgcaatat ttgtttcttt
    20101 gacccgatac cctttcactc aatcaggttt tatttctcta tagtctgcac atgtaagagt
    20161 agctttcgtc catgtctaac cagaggggaa caagatgaac ggactcacgg caacgggtgt
    20221 caccgtcggt atttgtgcag ggctttggca gttagtgtct tcacatgtcg ggctctctca
    20281 aggatgggaa ttattaggaa ccattggctt cgtcgccttt tgcagttttt atgccgcagg
    20341 cggcggtaaa tcggggttta tcagaagtct ggcggttaat tattccggga tggtgtgggc
    20401 gtttttcgcc gcgctgactg ctggttggct ggcctctgta agtggactgt ccgcgttctg
    20461 ggcgagcgta attactaccg tacccttctc ggcggtagtg gtctggcaag ggcgtttctg
    20521 gttactgtcc tttattcccg gtggctttct cggcatgacg ctgttttttg ccagcgggat
    20581 gaactggacg gtgacgttac tgggttttct ggcgggtaac tgcgtgggga taatttcaga
    20641 atatggtgga caaaaattaa gcgaagcgac gactaaacgc gacgggtatt aaaagttatt
    20701 caaaaatgat tatctatctt taaccgatac ctctacagtc atagctggag aaattcatag
    20761 tagataatgg cgagaatggt ttaatgaact caatttttac cgaagagaat ctgttggcat
    20821 ttactaccgc tgcgcgtttt ggcagcttca gtaaagccgc agaagagttg ggtttaacca
    20881 cttccgccat tagctacacc atcaagcgta tggagacggg gctggatgtg gtgctgttca
    20941 ctcgcagtac ccgcagcatt gagttaacgg agtccgggcg ttattttttc cgcaaagcta
    21001 ccgacctgct gaatgatttt tatgccatca aacgcagtat cgatactatt tcgcagggca
    21061 tagaagcgcg tgtgcgcatt tgtattaatc agctgcttta tacgcccaaa cacaccgcac
    21121 gcttgttaca ggtgctgaaa aagcagtttc ctacctgcca gattaccgtc accaccgaag
    21181 tgtataacgg cgtctgggat gcgattatta ataatcaggc caatatcgcc atcggcgcac
    21241 ccgatacact actggacggc ggcggtattg attacactga aattggcgcg attcgctggg
    21301 cttttgctat cgccccggat cacccgttag cctttgtgcc ggaacccatc gccgaaagcc
    21361 aactgcgtct ctaccctaat attatggtgg aggacaccgc gcatacgatt aataaaaaag
    21421 tgggctggtt gctgcacggg caggagtcaa ttctggtgcc agatttcaac accaaatgtc
    21481 agtgccagat tttaggtgaa ggaattggtt ttttgccgga ttacatggtc cgtgaggcga
    21541 tgacgcaatc cctgctggtt acccgacaaa tccataatcc gcgccaggat tcgcgcatgt
    21601 tactggcgac tcagcattct gctacgggtc aggtgacgca gtggattaaa aaacaatttg
    21661 cgccaaatgg catattaacg gggatctatc aggatttact gcatcgggag aattaatcgc
    21721 tgccaacctc ccgatgcgct gtgaatagcg gtttacatca accacctgct ttcattaccc
    21781 attcagagat aaatttatta cccggtactg gcggagagta caaatagccc tgcaaaaagt
    21841 gaacgccttt accgatcatt aaatccgcct gctcctgggt ttctacccct tccgccacga
    21901 tactcagacc aggcttacgc gctagttcga caatattgtc cacaatatga ccggagattt
    21961 cgtcgacact cgccatttgc acaaatgact tatcgatctt aataaaatcg accgggaacg
    22021 cctgcaagta acgataggtc gcataacccg taccaaagtc atccagcgca aaggtaatgt
    22081 tgtgctgatg aaggctgtca aatatcgctc tggcttctgg cgttaccgga atagggttac
    22141 gttccgttag ctcgagaacc agcttgattt tatcgttacc taatttctta accaggttca
    22201 gacactcttt ttcaaatccc gctgccaaaa aacaacccgc cgagacgttg atgccaatat
    22261 ggaaattgtc cggcagcaaa tgttttaccg gcatcagaat atccgcagtc tgtttcatca
    22321 gttggcgggt cattatgaca ataagaccgg atgactccgc cagaggaata aactgatccg
    22381 gtgggataat tcccgtttgt ggatgttccc agcggacaag tacctcacag cccgtcagta
    22441 ccccagtctg cgcgcagaaa accggttgga tccacggttt gaattcgtgg ttttccaacg
    22501 ccagactgat ggcttcaggc gtgacgatat ggtgacaacg gttatcaggg atataaaaat
    22561 tatttttatt tcccgtgcca ctgatgactt gcggatgata ttgaaaaaag atatcccgcc
    22621 agaaggtaat atcactctgg atgcctagtt tttcatacag ctgcttcttt tgatgtgaaa
    22681 ttgtctttgc actgcgattt ctgtactgtg agatttctgt aaccgacatt ccctccatta
    22741 gcagcttgag taaaagccgt tctttttcgg acacgatacc aggcaacgtc aaattcaccg
    22801 ttgccccgaa ctcttccaaa aaaacactca aatcatgcga agccattgaa cacaccttga
    22861 ttaaatcttg tgcgccagct aaatctgcga gtcttcactc tgactgataa agctggctgc
    22921 aagaaaatga cacagagagc cgaattatct cccgttcggc tctctctcct tttactcatt
    22981 attcagatca gaattgccac ttaatgccaa ccattgcaga ggtgtcatta tatcccctat
    23041 cggcaacctg aacgcccaca ttcccccaga cattcaggtt ggcatttagc tgtccttcca
    23101 ccccggtttt tatctcagca atatttcggg ctccatcctg agtgacggac acgccatcca
    23161 tactggtgct gaaatcctta ctgttatgca gccagttcac ttctacaaac ggctggaact
    23221 cgcgggattt accgtcatcc attttatggt ggctcttcag ccaggttttt acgccaagtc
    23281 gggtttgaac attgccatca ccgttgctat gaacgagggt tccgttgctt tcgcggtgct
    23341 tatcggcttt gactcccatc caggtaacct gtgcttgcgg ctgaacatac catgcattac
    23401 gcgttccctg gctgccatta aattcagcta atttgtgttt gtatccagct tccagtgaag
    23461 cggtaaatcc ttttgattta taggattcac tttgtaagtc atcccctttc actgtgttat
    23521 caaaccagct gtactgcgcc caactgtcga gatacgcgcc attacgcgac tcgtcatcgg
    23581 cataccaggt ggcatagagg ccggtgctat atccgttcac actcgctttt gcacgataac
    23641 cggttcgcga ggaaatggtt ttgctgtcgc tgttgccata tcccgccatg accccaacat
    23701 gccagctgtc gctgccgttt tggctccact gcgcgacatc gcctcccagt tgcagaacat
    23761 agcgattgct ttgggttttc agctggccgc tgccatcacg ccatttatta tgaccacctt
    23821 catggcgcat ccacatagtg gtttgtttct gctcacccgt caccatgtcg gtatagtacg
    23881 tattacccag acgctcatgc agacgcgtgg tgaacattgt attcgctgct gcaaggttcg
    23941 caatgtagct acccgcctcc ggtcgcaggt cattatccac attcagatcc ggacccggcg
    24001 taggtgtcgg gttagggttc ggctctggat tcgggtttgg ctccggattc ggcatcgggt
    24061 ctggctccgg ctgcagttca ggagaatcac tgccgctggt cagataccag ttaccactat
    24121 ttgccccttg tccacgcgcg agagtgtagt catacgcccc cgcaacgata cgaccagcct
    24181 gaacaaattc gccctcagac ttaccgtcta catggataag ttcgataccg ttaagggttt
    24241 tcgcacctgt accgccagcg ttattcaccg taacggcagt cgtgccgcta gtatcgccct
    24301 cgacaaccaa tttatcggtt actgagtcat cgccattcag taccgtattc attactagtt
    24361 gaccgttatt accgtggtag ttccccttca ccactaacac attgccagct tgctgtcctg
    24421 acattccggt catcagcgta ccgttgttgg tcagattgcc gcccagggtg taattcacag
    24481 acgcagccgc tcttttttca gcgctattat tcaacgccag ggtaccgttg ttctcaacgt
    24541 ttgcgctacc caatccctgc gcatgactta ccaggacatt agccgaatta gcaatgttca
    24601 cattcgctgt caggttgctg ttatcgcccg cgatgtccag cgtgccgccc tgaatattca
    24661 gctctccgct ccccgttaac gaaccggttg aagtgccccc gttggtaacc gtcagactgc
    24721 cgccatttaa gctcagcagt gaatcggcgc tgccattgag cgtgcccacg gtctggctgt
    24781 gaccattcat atccagttca gtctctgccg ccagacgcag ttcatgggta ttacccaaca
    24841 cgttatcgtt attcaacaaa agcgtcccac tgcgcagatc ggtaacaccg gtatagtcgt
    24901 tgtctttgtt agaaagcgat acggtctgac ccttctggct gctgaatgcc aggtcaccac
    24961 tgccggtaat ctttgcgctg agatcggcgg cattctcgct tttaccgtta gagctcagca
    25021 ccagagcatc gctgtcggta gcgtgtaaat caagctgggt caggccgtaa ccgatataca
    25081 gaccgtcatt gtttacccca ctcgtcagac gatagtcgta agtgccgacg gccgcttttt
    25141 gcccattctg ctgaatatca cgttgggcac tgctggagag gatttgccca tttttatcct
    25201 gcaattgcag attgcccgca tcgcccagaa cttccgcacc ttgcgcatca accagcttaa
    25261 tggcgctgtt accatcatcg acttcagtga gcgataacgc agagtcaata tcacgagata
    25321 ctgagcggac aacgtcactg tcagaaacct gaatcacacc ctcgccgcgc aaatccagcg
    25381 ttttactaac gttgaccgtc ccttctgtca tctgtgcacc tgctgtcagg gcaccaaaat
    25441 caacggttcc gccggagaat cccaaagtgc taatggagtg agcgccctta cccactgtta
    25501 cctggcttcc ctgacccgca gtgatcccgc ctctctgcag aaactcagca ttcttttcca
    25561 ggttaaatgt cgtgttctgg aacgccagtg aaccctggaa ccagtctgta taagcaaact
    25621 catcgaagga gaattcgtga ttccccatgt ttatttcaac aatacctcta ccttgaagat
    25681 cgttatcaaa tacccaatct ttattggcat tgatttgcag tttgccatca acatccaggt
    25741 ccgcacggcc gatgttcttg tcttcagaaa taaccgcgac agcaccagaa gcgacatcaa
    25801 tatatccgct gaatgcatag ttattgccga tcagtgccac tgtcccactg tcgatactta
    25861 ttgtaccatt gccggaaatc ttattggcaa aggtcatgct atcgtcagcc ttcaggtgta
    25921 atttcccgcg gttgttgatc tctgctgtac ctatattttg ggtgatccct aaagcgccat
    25981 tttgctcaat tttgacttta ccactaaagg cagagttatc accggttaag taagtcgtgc
    26041 cattgagcgc attgatatag cccaggccct cgctgccact gacaatatcc gcggaaatag
    26101 tcgcgtcttt atccgtgtgg tttaaggtga ttttcttatc ggatgtcgaa gcccacacaa
    26161 actcaatttt ttcggcatca ataatccctg ccgccttcgc agcacttcct tcctgtgcac
    26221 ctaacgctaa ctcagagttg gtgcttaaac taattttcgg tgcggatatt ttacctccat
    26281 ctgatacgac taatgttgta tcagaacgtt ggtcgtaata acccaggcta atatgttcac
    26341 gagcattaac ttcaccaccg ttattaaccg acaggaactg attaccgcca tatccgccgt
    26401 aaacgcccat aaagaaggtc cctgcattta cctgactacc ttcgccgctt acattcccct
    26461 gcgtataagt cagacgactt gtgcccttaa catgaatttt gccgccattg gagacattaa
    26521 aatagccttg atcatgtctc atgcctgagt ttgactcata caaatcatca gcatataact
    26581 cgctgccagc attggagaca ttcaggaacc ccgtaccttt cagcaaaaag ttaagtgggc
    26641 cattgatagt tacctttcct ccggtaatat ctaacccacc accaaaagcg agagtcagac
    26701 gcggacggga agtatctgtc gcattgatta ccagattggc attgtcagtc accgtaatac
    26761 ggtaatcgat gctctggttc tctttgccaa aatcaatagt tttatcctgg tttacgatga
    26821 catcatcggc gaataccaga gatgaatata aacttatcgc gctaattaaa acagcccggg
    26881 acttttttcc ttttactctg ctccccagct ccgagcagac gctccaacag ttacgggacc
    26941 tgttccactt tagccgatat attttattca tcacaatctc cagcaataca tccaaataaa
    27001 accaagattg cataaacagc aacgcattaa ataccattct ggttactgtt catcgcatat
    27061 aaaaattcaa aaattttgca tagcattggc aaccacatat ctgagtatgt agttcagagg
    27121 gtaataaaat agcaagttat tacttaaaac ttattttaag ttaacactct taactttatc
    27181 attacattcc tcccagtctc taatacctgc gcattatatg tatccaggaa ctcaaatcaa
    27241 ctcaaaatat aaacaaaagc aagaggaata catttaatat acagccgata aaagcggtaa
    27301 ctcttattag gtatcaaaat ataagaatcg tcttattaat aaatatataa cactatagga
    27361 ctgaatccat tcagctaata caaaataaaa tcgttaatta ataaaatata aaatcatgaa
    27421 gtgatacaag aggaattgca ggatatgaca tgacaatcat atataatata tgcataattt
    27481 tttacagcga gtcaatgatt cccgcgccat atgcagacat ggcgcggttt tatgcaataa
    27541 caatcacgcg tccgggaaca tcacgctatg gcccggcgct tcacgatgga gatggataaa
    27601 gttaaggtgc cgctcgtact gatcgagaat atcggtgatc acctgctctt tgctgtagtc
    27661 catcaggtcg ttgccctggc tgccttctaa caggaaagtt tccagccggt agtaggtcga
    27721 tttaccgcta cgtgcgcggt aggtaaagcc cggcaccgaa tattgctgcg gccaaatctg
    27781 atagacaaag ttttgctctt cgcccatatg caccaacaaa tccagatgcc ccaactgttg
    27841 tccctcttcc ggcggcaggc tttttagctc cacgtacgcg ccgcgtaacc gcagctcctg
    27901 cgccacttct tccattgccg ggtaacagac cgtctccatc atctgtttag tgtaacgcgt
    27961 gcccggataa ttcatcaggc gcgagagacg ttttttccag ctcaggcgat cctgaagccc
    28021 cagcggtcgc ggcgcggtat cacggttggc actttcacgg cggtaatctt ctaccttcag
    28081 agatttatac aaccccgcca tcacgaagaa aatcacaaag ctgaacggca gccccatgat
    28141 taccgtggtg ttttgcagcg cggatatccc gttggtcatc agcatgccaa gcgtcagcag
    28201 accaatcgcc accgaccaga agacgcgcag ccagccgggg gcgtcgctgt tgatatcttt
    28261 aagctgcgag gtgaaattcc ccagcaccag cgcaccggag tccgccgagg tcacataaaa
    28321 cagcaggcca gtaatggtgg cgacggaggc gctaaaggta aacgccggat actgcgccag
    28381 caggctgtag aagccgcgct ccggatggac catcgcttcc tcggcaaatg ccgcgccgcc
    28441 gtggatgatt tcatacagcg cgctattgcc gaacaccgag agccataaca gcgtgaaggt
    28501 aaacggaata atcaacgtgc ccagcacgaa ctggcgaatg gtacgcccac gcgagatacg
    28561 cgccaggaac aagccgacaa acggcgacca tgccacccac catgcccaga agaagagcgt
    28621 ccagttattc atccactcaa ctggacggtc gaaggcaaaa ctgttgagcg tcatgcccat
    28681 aaagcgattc acatagtcgc caacattcag caccagcgca ttaagcagga acgaggtgtc
    28741 gcccataaac aatacgaaca ggatcaatcc cagcgccagc gcaacgttaa gttccgataa
    28801 cacacgaatc cccttatcga caccggaggt gacagagatc gtggcgatta tcaccgacaa
    28861 ggcgatcagt gccgcttttg ccgccatcga atcgggaata tcaaacagta cgctcaagcc
    28921 atagttaagc tgcaccacac cgataccgag cgtagtggca ataccgaaga tggtgccgat
    28981 caccgctgca atatccactg agtgccctat cggcccgtta atccgtttac cgaagatcgg
    29041 gtacagcgcc gagcggatgg tgagcggcaa attataacga tagctaaagt atccgagcgc
    29101 catgcccatc agcgcataca tcgaccagcc ggttaagccg tagtgaaaca gcgtccagac
    29161 catcgcctga cgcgcggcct caatcgtctg tcccgcgcct tccggcggct gcatatactg
    29221 cgttaccggt tcggctacgg agaagaacat caggtcgata ccgatcccgg cagcaaacag
    29281 catcgccgcc caactcagca ggctgaattc cggtttggat tgttctggcc cgagcttcac
    29341 cgaaccaaaa cgcgaacaag cgatacagac cacaaagaca atatagagcg ttgccgccag
    29401 cagatagtac caaccgaagg ttttagacac ccagtccagc gtgcggccaa tccacagggc
    29461 cgagaagtcg cgaaacagga tcgttgtcag ggaaaacaac aaaatcagtc cggcggaggt
    29521 gtaaaacacc accggattga ttttgtcctt ttccctgctg tgtgaaaggt ctgtcatcca
    29581 gtatccccac tgttattgtt accatttaaa atcaaattcg taacaattaa gacacatttt
    29641 atattgaacg tccaatcaat aaccgcttta atagataaac aacgctgatg aatggagtgg
    29701 cgaaaatgcc caaattgggg atgcagtcga tccggcgcag acaactgatc gacgccacac
    29761 tggaagcaat aaatgaagtg ggcatgcacg atgcaacgat cgcgcagatc gcccgccgtg
    29821 caggcgtttc tacggggatc atcagccact atttcaggga caaaaatggt ctgctggaag
    29881 caaccatgcg cgacatcacc agtcagttac gtgacgcggt tttgaatcga ttacatgcac
    29941 ttccgcaggg cagtgcagag cagcgattac aggcgattgt tgccggaaac ttcgatgaaa
    30001 ctcaggtgag cagtgcggcg atgaaagcct ggctggcgtt ctgggccagc agtatgcatc
    30061 agccgatgct ctatcgttta cagcaggtca gcagccgccg cttgctgtcg aatctggtga
    30121 gcgagtttcg ccgcgaattg ccgcgcgaac aggcacagga agcgggctac ggccttgccg
    30181 cgctgattga cggattatgg ctgcgcgcgg cactaagcgg caaaccgctg gataaacctc
    30241 tcgcacactc gctgacccgt cactttatta ctcagcattt acccaccgat taaccgagga
    30301 gacgtgatgt cccgaatggc agaacagcag ctttatatac atggtggtta tacctccgcc
    30361 accagcggtc gcaccttcga gaccattaac ccggccaacg gtaacgtgct ggcgaccgtg
    30421 caggccgccg ggcgtgagga tgtcgatcgc gccgtgaaga gtgcccaaca ggggcaaaaa
    30481 atctgggcgg cgatgaccgc tatggagcgc tcgcgtattc tgcgtcgggc cgttgatatt
    30541 ctgcgtgaac gcaatgacga actcgcaaaa ctggaaaccc tcgataccgg aaaagcatat
    30601 tcggaaacct caaccgtcga tgtcgttacc ggtgcggacg tgctggagta ctacgccggg
    30661 ctgatcccgg cgctggaagg cagccagatc ccgttgcgtg agacgtcatt tgtttatacc
    30721 cgccgcgaac cgctgggtgt ggtggcgggg attggcgcat ggaactaccc gattcagatt
    30781 gccctgtgga aatccgcccc ggcgctggcg gcaggcaacg caatgatttt caaaccgagc
    30841 gaagtcaccc cgcttaccgc gttaaagctg gctgaaattt acagcgaagc gggcctgccg
    30901 gacggcgtat ttaacgtgtt gccgggggtg ggcgcggaga ccgggcaata tctgaccgag
    30961 catccgggca ttgccaaagt gtcatttacc ggcggtgtcg ccagcggcaa aaaagtgatg
    31021 gctaactcgg cggcctcttc cctgaaagaa gtgaccatgg aactgggcgg taaatcaccg
    31081 ctgatcgttt tcgatgatgc ggatctcgat ctcgccgccg atatcgccat gatggcaaac
    31141 ttcttcagct ccggtcaggt gtgtaccaat ggcacccgcg tcttcgttcc ggcgaaatgc
    31201 aaagccgcat ttgagcagaa aattctggcg cgcgttgagc gcattcgcgc gggcgacgtt
    31261 ttcgatccgc aaactaactt cggcccgctg gtcagcttcc cgcatcgcga taacgtgctg
    31321 cgctatatcg ccaaaggcaa agaggaaggc gcgcgcgtac tgtgcggcgg cgatgtactg
    31381 aaaggcgatg gcttcgataa cggcgcatgg gttgcaccga ccgtgttcac cgattgccgc
    31441 gacgaaatga ccatcgtgcg cgaagagatc ttcggtccgg tgatgtcgct tctcacttat
    31501 gaatctgagg acgaagtgat tcgccgcgcc aatgataccg actacggcct ggcggcgggt
    31561 atcgtgacgg cggacctgaa ccgcgcgcat cgcgtcattc atcagctgga agcgggtatt
    31621 tgctggatca acacctgggg cgaatccccg gcagagatgc ccgttggcgg ctacaaacac
    31681 tccggcattg gtcgcgagaa cggcgtgatg acgctccaga gttacaccca ggtgaagtcc
    31741 atccaggttg agatggctaa attccagtcc atattctaac caggaggttt atttgcaatt
    31801 tgactacatc attattggtg ccggctcagc cggcaacgtt ctcgctaccc gtctgactga
    31861 agatccgaat acctccgtgc tgctgcttga agcgggcggc ccggactatc gctttgactt
    31921 ccgcacccag atgcccgcag cgctggcgtt cccactacag ggcaaacgct acaactgggc
    31981 ctatgaaaca gagcctgaac cgtttatgaa taaccgccgc atggagtgcg gacgcggtaa
    32041 aggcctgggt ggatcgtcgc tgatcaacgg catgtgctac atccgtggca atgcgctgga
    32101 tctcgataac tgggcgcaag aacccggtct ggagaactgg agttatctcg actgcctgcc
    32161 ctactaccgc aaggccgaga ctcgcgatgt gggcgagagc gactaccacg gtggcgatgg
    32221 cccggtgagc gtcactacct ccaaacccgg cgtcaatccg ctgtttgaag cgatgattga
    32281 agcgggcgtg caggcgggct acccgcgcac ggacgatctc aacggttatc agcaggaagg
    32341 ttttggtccg atggatcgca ccgtcacgcc gcagggccgt cgcgccagta ccgcgcgcgg
    32401 ttatctcgat caggccaaat cgcgccctaa cctgaccatt cgtactcacg ctatgaccga
    32461 tcacatcatt tttgacggca aacgcgcggt gggcgtcgaa tggctggaag gcgacagcac
    32521 catcccaacc cgcgcaacgg ccaacaaaga agtgctgtta tgtgcaggcg cgattgcctc
    32581 accgcagatc ctgcaacgct ccggcgtcgg caacgctgaa ctgctggcgg agtttgatat
    32641 tccgctggtg catgaattac ccggcgtcgg cgaaaatctt caggatcatc tggagatgta
    32701 tctgcaatat gagtgcaaag aaccggtttc cctctaccct gccctgcagt ggtggaacca
    32761 gccgaaaatc ggtgcggagt ggctgtttgg cggcactggc gttggtgcca gcaaccactt
    32821 tgaagcaggc ggatttattc gcagccgaga ggaatttgcg tggccgaata ttcagtacca
    32881 tttcctgcca gtagcgatta actataacgg ctcgaatgcg gtgaaggagc acggtttcca
    32941 gtgccacgtc ggctcaatgc gctcgccaag ccgtgggcat gtgcggatta aatcccgcga
    33001 cccgcaccag catccggcaa ttctgtttaa ctacatgtca cacgagcagg actggcagga
    33061 gttccgcgac gcaattcgca tcacccggga gatcatgcat caaccggcgc tggatcagta
    33121 tcgtggccgc gaaatcagcc ccggcacgga atgtcagacg gatgagcagc tcgatgagtt
    33181 cgtgcgtaat cacgccgaaa ccgccttcca tccgtgcggt acctgcaaaa tgggttacga
    33241 cgagatgtcc gtggttgacg gcgaaggccg cgtgcatggg ctggaaggcc tgcgcgtggt
    33301 ggatgcgtca attatgccgc agattatcac cgggaatttg aacgccacga caattatgat
    33361 tggcgagaaa atagcggata tgattcgtgg acaggaagcg ctgccgagga gcacggcggg
    33421 atattttgtg gcaaatggga tgccggtgag agcgaaaaaa tgagtcgtga tgtgaactaa
    33481 cgcaggaacc gatcagattg agagttaccg ttccagagag ggggaccgaa tccttatata
    33541 aacactgagg taactctcat gcttcatatc cagtattcat gatgctggtt ttttgctgat
    33601 atttaccccg aacattcctg atacagacta ttaatacaac cttatctgcg gcagatttta
    33661 ttttctggat atattatgag tgaacaaatc aaacaggata tcgatctgat tgagatttta
    33721 ttttatctga agaaaaagat tcgtgttatc ctttttatta tagctatctg tatggcaatg
    33781 gtgcttttat ttctgtatat caataaagat aatataaaag tgagttacag cctaaaaata
    33841 aaccagacaa caccaggcat acttgttaac tgtgatagca ataataactt tgcctgtcag
    33901 accacaatga ctgaagatgt tatacagcga attactacat tttttcacac cagcccagat
    33961 gtcaagaaca gagaaataaa gctggaatgg tcaggagata agagagattt accaactgct
    34021 gaagcggaaa tatctcgcgt gcaggcgtct attatcaaat ggtatgcgtc agaataccat
    34081 aatggcaggc aagttctcga tgagatacaa ataccttcag caattaacag tgagctttat
    34141 acaaaaatga tatacctgac caggaactgg tcattgtatc cgaacggtga tggctgtgta
    34201 actataagct caccagaaat aaaaaataaa taccctgctg ccatttgcct ggctctggga
    34261 ttttttctaa gcattgtaat ttctgtaatg ttttgccttg tcaaaaaaat ggtagatgaa
    34321 taccaacaaa actctgggca gtaataaaca gcattctgat agagagtgcc tgtataaagc
    34381 tgaagtgaca atgaccggga agggaacccg ttttcaggaa tggatgcctg tctttttcac
    34441 tgtaaatatc ctggcattac taatgccagg atatttacag aagagcagca taaccatgac
    34501 ggaacggggt taagtcaggt tgtccttaac gctattttca acctcgtatg ccttcttcag
    34561 gtttatgttc agatctcata tctctctcag caatcctcaa taatcagata caccgctttc
    34621 accgggccat gaaccccgac gactttgata agctcaatat ccgccgttga actagggccg
    34681 ctaatgatgt taatgcagga cggcattcgt tcaccggcct gcgctttctg atgcaatttt
    34741 tctgcgagtt gcgctacacg cggcaggatt gtgcttttac gcaggataaa aagagaagat
    34801 tccgggagca ggctcaatga acgcccgcgc ccggcggcgg aaaaaagaac tacgcctccc
    34861 gattcggtta aaccatattc agcatacacg acacccactt tagcctgctc ggcctgcgag
    34921 atattctcgg cacctttcgc cggatcccaa acaacggcat tgcattcctg ctgcaaacgt
    34981 tcactaatcc ccaattcctc cagcctcgtg tcaccgctaa tcacgaccga ctgatctccc
    35041 agctctttac acagacgtat tgcagcttct gccgccttcg cctcgctggt cagctcacag
    35101 cgcgtcaaca tcacatcgct ggcaaactga ataaacgcgt cacagcgctg ctgttggtta
    35161 agttgggtaa gccgctcgtt agcatagttg ttaagcggcg catcttctgc ttgcggttca
    35221 agtcgcaacg ggcgacccag tgcctgagca acgttattca aaaattcgct ccgattatcc
    35281 attctttttc tcctgcgcct gatgtttctt aaaccaacta cggaaactct ctccgtcagc
    35341 ttcaggaaga tcgcgtgctt ccatccagtc gctaatcgcg ccaaatttga gtggtgtttt
    35401 gccgccattg ataaaccagc ttgtcgcatg agcaccggcc atcatcccga ctttccacaa
    35461 tcctggatga ctattggcat aagcgaacat ttttatcgcc cgttgctctg cttttgcggt
    35521 gatccctttt tcagccatca cccgacgatg acgcaaaatc agttttgaca gcggaatacg
    35581 caccggacac acgctgtcac aagcagtgca taaagagcag gcgtagggta aatctttaaa
    35641 atctttatag ccgccaagta gcggagaaat caccgcacca attggccctg gataaataga
    35701 gccatatcca tgaccgccaa tatggcgata tgccggacaa gtattcatac aagccccgca
    35761 gcgaatacag cgcagcacat cccgaaattc agaggccagc acctcagaac gcccgttatc
    35821 gacaataacc agatgaaact cttcaggacc atcaacgtgc cccgcttcgc gcggtcctgt
    35881 cagccaggtg ttgtatcccg tcaaacgtgc accaacggca ctgcgcgcca gcatggtgat
    35941 caatacatct acctcggcaa acgtgggggc aatacgctcc attcccatca ctgcaatatg
    36001 cgttttaggc agcgtggtac acattcgcgc attaccttca ttggtcacca ggcataccga
    36061 acctgtctct gccaccgcga aattacagcc ggtaatacct atttcagcac tgaggaaatc
    36121 ttcgcggatt ttttgccgga tgaataaggt catcgcttca ggcgtttccg gcccctcata
    36181 gcccagacgt tcgtgtagca ctcgacggat ctgatggcga tctttatgaa ttgccgggac
    36241 cacaacatga gatggcggat cttgatccag ctggagaata tattcaccca gatcggtttc
    36301 aatcacctga atgccagcat cctgcaacac atgattgaca ccaatctctt cggtcaccat
    36361 cgatttagat ttcaccacct tccgggcatt tttgcgttgg gcaacctgta aaatgtagcg
    36421 ggtagcgtct tctttggttt ttgcaaacca gacgtgaccg ccgttttgcg tcactttttc
    36481 tgagagctgg tacagataag cgtcgagatt actcagaaca tgatcacgta tctgggcggc
    36541 ccgatcgcgc cactcctccc agtgccccaa ttcatcgacc attttttgcc gatttgcccc
    36601 aatacgctgc tgcgcgtttg ccaccgcttt gcgcatgatc ggatcttcaa tttgctgacg
    36661 gatgcgtgtc ttaaaatctg tattactggt tttgatcgac atctttatat cctcagcggc
    36721 tcatcaacac ttcagcaata tgcatcactt tgactttctg cccttcccgt tgtaatcgcc
    36781 cactgatgtt cagcaggcaa ctcacgtcag caccaattaa atactcaggg cggacttcca
    36841 tcaggtgcac aaccttttct ttcaccatct cgccggatat ttcggccatt ttgaccgaga
    36901 acgtgccgcc aaatccgcag caggtatcct gttcagcaaa ggtcaacagc tccagtccac
    36961 gcacattttt cagcagcgta agtggctcgt ccttcactcc cagcttacgg gccaggctac
    37021 aagatgggtg atacacagct ctcccttgca aactggcacc tacatcgact acccctaatt
    37081 tattaacaat aaaagaggtg agatcctgca tacgcgcggc aaccttttcg gcacgtgatg
    37141 cccattcagg ttcatccgcc agatacatcg ggtaactttt tacggcatag gtgcaggagc
    37201 cagccggtga aataatggga tcatcgttat cctccagtgc ggcgatcaga tttttcatcc
    37261 ctggaatcgc ttcattgata taaccactat tgatcgcagg ctgaccgcag catccctgtt
    37321 tctccgggaa atttacgcga cagccgagtt tttccagtag cagcacggag tctcgtgcca
    37381 ttcttgattt cagggcgtca ccaatacagg tgacaaagaa attgacattc acaactaata
    37441 ctccattact tcatgcccat ttatgcccga tggttccccc cttccggggg agcaatcatc
    37501 aataaatatg gcgttctttt ttaattggct agcaacatac cacccctcat ttgtatgaca
    37561 aatgataatc tttcatccaa acttcaaatg caataactaa ttttatttct ttcggcatcg
    37621 ctcacatttt atggggaaat cgacgatcat ttttggcata agaaatccat ttaaaaatgg
    37681 ccacatccct tatacctatt cagagctact tattcagggg ttattgagat atacactgat
    37741 ggtgtacaaa caaaatcacc gacaataatc acgtatcaag taatagtcgg gattttattt
    37801 aattgaagca atttagtttg tatcttttat ttaattacta agccagacca cttcaaaaaa
    37861 acatataaaa aagttggaaa tataaatagc tgaaaggctc acaacacccg gaatgcggat
    37921 tatctttgct ccgattcctg gcggttttat tcagggtgca agctgtctga ccctttcccg
    37981 atattctccc ggggtacaac caaactcgcg gacaaacgcc ttgtgaaaag atgattcact
    38041 ggcatagcct actgactcag cgatcaccac aacagggagc gtttcccggg aaaacatctg
    38101 ggccgctatt tgtagacgca actttgttaa tacagccagc ggcgtggttc cggaaacatc
    38161 acggaaaagc tgggcaaaac ttgcccggga catgtgggcg atgctggcca gcgattcgac
    38221 ggtccaggcg tgtcctggca tttccagcat ttgctgtatt accgcaccaa gacgtggatg
    38281 cagaagcaaa ctgagaatgt ttttctctgt attaacctgt gcaatccact cacgcaccgc
    38341 gagggtaaag aatgtagcgc agatctggct acacagagca tccattcccg gcattgccga
    38401 tctggattcc tgttgtaaaa acgggatcgc ctccctcagc cagttatatt ccacgctgtg
    38461 gttaaccggt gctaaaaaca gcgtttccgg caaagacgtt aaaaaataac gcgccgaatg
    38521 ttgcaaccga agagtgccgc agacaataca ggtcgattca ttatcgacat gactcaggcg
    38581 atgagctgaa ttttgtggta gcaggaccac atttcccggg cgtaatgtaa aaatctctcc
    38641 cgtcggcatt tccagcttcg ccgctccttg cgttaacgca tgccaacgaa taaccgataa
    38701 ttccccggca ccatgtggaa gctgccagtc acttcctaac acgcaattct tatcgatcgt
    38761 tccttgtgga gcgttaagca tcaacaaacg gctaagggca tccatatcag actcctgagt
    38821 ggtttttgat tacaaacacc aacaatctta gtaaataact aatctaatac catcaaatgg
    38881 ttaatcatga tttttgtttt gtgattctga ttaagaaaaa gaaaatacca gacgataaga
    38941 caaaatatca agcgttgtga agaaatggta tttgctcttt tgcgtctata gtcatgatgt
    39001 caaatgaacg cgtttcgaca ggaaatcatc atgaataaat atcaggcagt tattattggt
    39061 tttggcaagg ctggaaaaac attagccgtc acgctggcaa aagcaggttg gcgagtggct
    39121 ctcatcgaac aatcaaatgc aatgtatggc aggacctgta ttaatatcgg ctgcatccca
    39181 accaaaacat tggttcatga cgcacagcag cacacagatt ttgtccgtgc catacagcgt
    39241 aaaaatgaag tggttaattt tttacgtaat aagaattttc ataatcttgc ggatatgccc
    39301 aatatcgacg tgatcgacgg ctaggcggag tttatcaata atcatagcct gcgtgttcat
    39361 cggcctgagg gaaatctgga aattcatggc gagaaaattt ttattaatac cggtgcacaa
    39421 accgtggttc cgccaattcc tggaattacc accacgccta gtgtatatga cagcaccgga
    39481 ttacttaatc taaaagaatt gcctgggcat ttaggtattt tgggcggcgg atatattggc
    39541 gttgagttcg cctctatgtt cgctaatttt ggcagcaagg taaccatttt agaagcagct
    39601 tcgctgtttt tgcctcggga agatcgggat attgctgata atatcgcgac gattttacgc
    39661 gatcagggcg tcgatattat cctcaatgcc catgtggagc gaatcagtca ccatgaaaat
    39721 caagtgcaag tgcatagcga gcacgcccaa ctggcggtgg atgcactgtt aatagcttcc
    39781 ggtcgtcaac cggctaccgc ttcgttacat ccagaaaatg ccggtatcgc agtaaacgag
    39841 cgcggggcaa ttgtcgttga caagcaatta cataccaccg cagacaatat ttgggcgatg
    39901 ggagatgtta ccggcgggct gcaatttact tatatttcac tggatgatta ccgcattgtg
    39961 cgtgatgagt tgctgggaga aggcagacgt agtactgatg atcggaaaaa tgtgccttat
    40021 tccgtattca tgacaccgcc actgtccagg gttggtatga cagaagaaca ggccagagag
    40081 agtggtgctg atattcaggt ggtgacattg cctgtagctg caattccgcg tgccagagtg
    40141 atgaatgata ctcgtggggt attaaaagcg attgttgata ataaaaccca acgtatatta
    40201 ggggcatcac tgctgtgtgt tgactcccac gagatgatca atatagtgaa aatggtgatg
    40261 gatgccgggc tgccttatag catattacgc gatcagatat ttactcatcc gtcgatgagc
    40321 gaatcactca atgatctatt ttcattagtc aatgaaactc aaaatcagac gccagaacaa
    40381 atattctggc gtctcagaga aaagaatctt attaattccc tgttactcta taacttctta
    40441 atcacttcat tgatggtatt ttatatgttt aaaaaatctg ttttatttgc aacactatta
    40501 tctggcgtta tggcattttc caccaatgca gatgataaaa taattctgaa acatatcagc
    40561 gtctcgtcag tatcagcatc accgacagtt ctggaggatg ccattgctga tatagccaga
    40621 aaatataatg cttcatcctg gaaagtcaca tcgatgcgaa ttgataataa ttcaaccgca
    40681 acagcagtat tgtataaata aggatgttca caatggaaaa atacctgcac ctgttaagtc
    40741 ggggagataa aattggcctg acattgattc gtctgagtat tgcaattgtt tttatgtgga
    40801 ttgggttatt aaagtttgtc ccttacgagg cagacagcat tacaccattc gtcgcaaaca
    40861 gtccactaat gtcgttcttt tatgaacacc cggaagacta taaacagtat ctgactcacg
    40921 aaggcgaata caaaccagaa gcaagggcat ggcaaacggc caataatacc tatggttttt
    40981 ccaacggtct tggcgtcgtg gaggtgatta ttgctctgct ggttttggct aatcctgtca
    41041 atcgctggtt aggtttattg ggaggactga tggcatttac cacgccgctg gtaacactct
    41101 catttttaat caccaccccg gaggcatggg tacccgcatt gggtgacgct catcatggtt
    41161 tcccttattt atccggtgct ggtcgcctgg tattgaaaga tactctgatg ctggcaggtg
    41221 ctgtcatgat aatggcagat tcggcgcggg aaattcttaa acaacgcagt aatgaatcca
    41281 gttcaacgtt aaaaactgaa tattgataat ccgaacactg tctgttgttc actttttcag
    41341 gcgtcaggcc ccctttctga tgatgaagcc tgacgccata ttaccggaga tatgacagag
    41401 tgaagatcaa tatcactccc ctgttaaaag attcttgtcg tctcaggtaa aatatatgat
    41461 taatatcaat catgtatttt tacagcaaga caaccacgaa caaattcagg attaaaatgt
    41521 tttaccgact ccccaacata acccaaatca agcgaactaa tttgtgccat ttctttctct
    41581 gtcagtgaga aatcccagat agcaaaattt tcttcgatac gctgttgatg tgtcgatttc
    41641 ggaataacga ccactccccg ttgaatattc caacgcagaa tgacttgaga aatcgatttt
    41701 tgatgggcat ctgcaatact ctgaagcaga ttattttcaa agggtttatg tcgtccacca
    41761 cctaacggag cccatgcttc aggctgcacg ttataatact tcatggttgc cagcgcctct
    41821 ggttgggcaa aatagggatg caactcgacc tggttgacca tcggtttaac tcttaccgtt
    41881 tcacaaaagt tcgccagaac atgaggatag aaattggaaa ccccaattgc ttttaatttg
    41941 ccctcttcat aggcatcttc gagtgcacgc cacgcactga aataatcgcg catagcctgg
    42001 tgcaataaat agaggtcgaa gtactctagt cccgattttt ttaatgatgc ttcaatacct
    42061 gctgctgccg tatcctgatt caacatatcc tgcacccaca atttagacgt tataaataa
//
LOCUS       DAZKHC020000032        41420 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZKHC020000032 DAZKHC020000000
VERSION     DAZKHC020000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 41420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.032"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            31..738
                     /locus_tag="ACHOSM_RS10095"
                     /old_locus_tag="pgaptmp_002019"
     CDS             31..738
                     /locus_tag="ACHOSM_RS10095"
                     /old_locus_tag="pgaptmp_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4558549.1"
                     /translation="MDMESQKLLFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
     gene            735..1442
                     /locus_tag="ACHOSM_RS10100"
                     /old_locus_tag="pgaptmp_002020"
                     /pseudo
     CDS             735..1442
                     /locus_tag="ACHOSM_RS10100"
                     /old_locus_tag="pgaptmp_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415179.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
     gene            1440..1649
                     /locus_tag="ACHOSM_RS10105"
                     /old_locus_tag="pgaptmp_002021"
                     /pseudo
     CDS             1440..1649
                     /locus_tag="ACHOSM_RS10105"
                     /old_locus_tag="pgaptmp_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            1646..3097
                     /gene="djlC"
                     /locus_tag="ACHOSM_RS10110"
                     /old_locus_tag="pgaptmp_002022"
                     /pseudo
     CDS             1646..3097
                     /gene="djlC"
                     /locus_tag="ACHOSM_RS10110"
                     /old_locus_tag="pgaptmp_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(3157..4827)
                     /gene="hscC"
                     /locus_tag="ACHOSM_RS10115"
                     /old_locus_tag="pgaptmp_002023"
     CDS             complement(3157..4827)
                     /gene="hscC"
                     /locus_tag="ACHOSM_RS10115"
                     /old_locus_tag="pgaptmp_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HGU4558550.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(4911..5846)
                     /gene="rihA"
                     /locus_tag="ACHOSM_RS10120"
                     /old_locus_tag="pgaptmp_002024"
     CDS             complement(4911..5846)
                     /gene="rihA"
                     /locus_tag="ACHOSM_RS10120"
                     /old_locus_tag="pgaptmp_002024"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HGU4558551.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAALDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(5964..6689)
                     /gene="gltL"
                     /locus_tag="ACHOSM_RS10125"
                     /old_locus_tag="pgaptmp_002025"
     CDS             complement(5964..6689)
                     /gene="gltL"
                     /locus_tag="ACHOSM_RS10125"
                     /old_locus_tag="pgaptmp_002025"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HGU4558552.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVINDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(6689..7363)
                     /gene="gltK"
                     /locus_tag="ACHOSM_RS10130"
                     /old_locus_tag="pgaptmp_002026"
     CDS             complement(6689..7363)
                     /gene="gltK"
                     /locus_tag="ACHOSM_RS10130"
                     /old_locus_tag="pgaptmp_002026"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HGU4558553.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSQKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(7363..8103)
                     /gene="gltJ"
                     /locus_tag="ACHOSM_RS10135"
                     /old_locus_tag="pgaptmp_002027"
     CDS             complement(7363..8103)
                     /gene="gltJ"
                     /locus_tag="ACHOSM_RS10135"
                     /old_locus_tag="pgaptmp_002027"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HGU4558554.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLSRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(8273..9181)
                     /gene="gltI"
                     /locus_tag="ACHOSM_RS10140"
                     /old_locus_tag="pgaptmp_002028"
     CDS             complement(8273..9181)
                     /gene="gltI"
                     /locus_tag="ACHOSM_RS10140"
                     /old_locus_tag="pgaptmp_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HGU4558555.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PYNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            9636..11513
                     /locus_tag="ACHOSM_RS10145"
                     /old_locus_tag="pgaptmp_002029"
                     /pseudo
     CDS             9636..11513
                     /locus_tag="ACHOSM_RS10145"
                     /old_locus_tag="pgaptmp_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
     gene            complement(11591..13129)
                     /gene="lnt"
                     /locus_tag="ACHOSM_RS10150"
                     /old_locus_tag="pgaptmp_002030"
     CDS             complement(11591..13129)
                     /gene="lnt"
                     /locus_tag="ACHOSM_RS10150"
                     /old_locus_tag="pgaptmp_002030"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HGU4558556.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QTLTVKRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSTLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(13154..14032)
                     /gene="corC"
                     /locus_tag="ACHOSM_RS10155"
                     /old_locus_tag="pgaptmp_002031"
     CDS             complement(13154..14032)
                     /gene="corC"
                     /locus_tag="ACHOSM_RS10155"
                     /old_locus_tag="pgaptmp_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HGU4558557.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(14122..14589)
                     /gene="ybeY"
                     /locus_tag="ACHOSM_RS10160"
                     /old_locus_tag="pgaptmp_002032"
     CDS             complement(14122..14589)
                     /gene="ybeY"
                     /locus_tag="ACHOSM_RS10160"
                     /old_locus_tag="pgaptmp_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGU4558558.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(14586..15626)
                     /gene="ybeZ"
                     /locus_tag="ACHOSM_RS10165"
                     /old_locus_tag="pgaptmp_002033"
     CDS             complement(14586..15626)
                     /gene="ybeZ"
                     /locus_tag="ACHOSM_RS10165"
                     /old_locus_tag="pgaptmp_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGU4558559.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(15779..17203)
                     /gene="miaB"
                     /locus_tag="ACHOSM_RS10170"
                     /old_locus_tag="pgaptmp_002034"
     CDS             complement(15779..17203)
                     /gene="miaB"
                     /locus_tag="ACHOSM_RS10170"
                     /old_locus_tag="pgaptmp_002034"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HGU4558560.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            17349..18524
                     /gene="ubiF"
                     /locus_tag="ACHOSM_RS10175"
                     /old_locus_tag="pgaptmp_002035"
     CDS             17349..18524
                     /gene="ubiF"
                     /locus_tag="ACHOSM_RS10175"
                     /old_locus_tag="pgaptmp_002035"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HGU4558561.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARNYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(18678..18752)
                     /locus_tag="ACHOSM_RS10180"
                     /old_locus_tag="pgaptmp_002036"
     tRNA            complement(18678..18752)
                     /locus_tag="ACHOSM_RS10180"
                     /old_locus_tag="pgaptmp_002036"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18718..18720),aa:Gln,seq:ctg)
     gene            complement(18790..18864)
                     /locus_tag="ACHOSM_RS10185"
                     /old_locus_tag="pgaptmp_002037"
     tRNA            complement(18790..18864)
                     /locus_tag="ACHOSM_RS10185"
                     /old_locus_tag="pgaptmp_002037"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18830..18832),aa:Gln,seq:ctg)
     gene            complement(18913..18989)
                     /locus_tag="ACHOSM_RS10190"
                     /old_locus_tag="pgaptmp_002038"
     tRNA            complement(18913..18989)
                     /locus_tag="ACHOSM_RS10190"
                     /old_locus_tag="pgaptmp_002038"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18953..18955),aa:Met,seq:cat)
     gene            complement(19005..19079)
                     /locus_tag="ACHOSM_RS10195"
                     /old_locus_tag="pgaptmp_002039"
     tRNA            complement(19005..19079)
                     /locus_tag="ACHOSM_RS10195"
                     /old_locus_tag="pgaptmp_002039"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19045..19047),aa:Gln,seq:ttg)
     gene            complement(19114..19188)
                     /locus_tag="ACHOSM_RS10200"
                     /old_locus_tag="pgaptmp_002040"
     tRNA            complement(19114..19188)
                     /locus_tag="ACHOSM_RS10200"
                     /old_locus_tag="pgaptmp_002040"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19154..19156),aa:Gln,seq:ttg)
     gene            complement(19212..19296)
                     /locus_tag="ACHOSM_RS10205"
                     /old_locus_tag="pgaptmp_002041"
     tRNA            complement(19212..19296)
                     /locus_tag="ACHOSM_RS10205"
                     /old_locus_tag="pgaptmp_002041"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19260..19262),aa:Leu,seq:tag)
     gene            complement(19305..19381)
                     /locus_tag="ACHOSM_RS10210"
                     /old_locus_tag="pgaptmp_002042"
     tRNA            complement(19305..19381)
                     /locus_tag="ACHOSM_RS10210"
                     /old_locus_tag="pgaptmp_002042"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19345..19347),aa:Met,seq:cat)
     gene            complement(19761..21425)
                     /gene="asnB"
                     /locus_tag="ACHOSM_RS10215"
                     /old_locus_tag="pgaptmp_002043"
     CDS             complement(19761..21425)
                     /gene="asnB"
                     /locus_tag="ACHOSM_RS10215"
                     /old_locus_tag="pgaptmp_002043"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HGU4558562.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21672..21770
                     /locus_tag="ACHOSM_RS10220"
                     /old_locus_tag="pgaptmp_002044"
                     /pseudo
     CDS             21672..21770
                     /locus_tag="ACHOSM_RS10220"
                     /old_locus_tag="pgaptmp_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21821..22573)
                     /gene="nagD"
                     /locus_tag="ACHOSM_RS10225"
                     /old_locus_tag="pgaptmp_002045"
     CDS             complement(21821..22573)
                     /gene="nagD"
                     /locus_tag="ACHOSM_RS10225"
                     /old_locus_tag="pgaptmp_002045"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HGU4558563.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(22621..23841)
                     /gene="nagC"
                     /locus_tag="ACHOSM_RS10230"
                     /old_locus_tag="pgaptmp_002046"
                     /gene_synonym="nagR"
     CDS             complement(22621..23841)
                     /gene="nagC"
                     /locus_tag="ACHOSM_RS10230"
                     /old_locus_tag="pgaptmp_002046"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HGU4558564.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            complement(23850..24998)
                     /gene="nagA"
                     /locus_tag="ACHOSM_RS10235"
                     /old_locus_tag="pgaptmp_002047"
     CDS             complement(23850..24998)
                     /gene="nagA"
                     /locus_tag="ACHOSM_RS10235"
                     /old_locus_tag="pgaptmp_002047"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU4558565.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            complement(25058..25858)
                     /gene="nagB"
                     /locus_tag="ACHOSM_RS10240"
                     /old_locus_tag="pgaptmp_002048"
     CDS             complement(25058..25858)
                     /gene="nagB"
                     /locus_tag="ACHOSM_RS10240"
                     /old_locus_tag="pgaptmp_002048"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGU4558566.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            26191..28137
                     /gene="nagE"
                     /locus_tag="ACHOSM_RS10245"
                     /old_locus_tag="pgaptmp_002049"
     CDS             26191..28137
                     /gene="nagE"
                     /locus_tag="ACHOSM_RS10245"
                     /old_locus_tag="pgaptmp_002049"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HGU4558567.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGVEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            28341..30005
                     /gene="glnS"
                     /locus_tag="ACHOSM_RS10250"
                     /old_locus_tag="pgaptmp_002050"
     CDS             28341..30005
                     /gene="glnS"
                     /locus_tag="ACHOSM_RS10250"
                     /old_locus_tag="pgaptmp_002050"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HGU4558568.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            30451..31857
                     /gene="chiP"
                     /locus_tag="ACHOSM_RS10255"
                     /old_locus_tag="pgaptmp_002051"
     CDS             30451..31857
                     /gene="chiP"
                     /locus_tag="ACHOSM_RS10255"
                     /old_locus_tag="pgaptmp_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HGU4558569.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            31907..32233
                     /gene="chiQ"
                     /locus_tag="ACHOSM_RS10260"
                     /old_locus_tag="pgaptmp_002052"
     CDS             31907..32233
                     /gene="chiQ"
                     /locus_tag="ACHOSM_RS10260"
                     /old_locus_tag="pgaptmp_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HGU4558570.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(32318..32764)
                     /gene="fur"
                     /locus_tag="ACHOSM_RS10265"
                     /old_locus_tag="pgaptmp_002053"
     CDS             complement(32318..32764)
                     /gene="fur"
                     /locus_tag="ACHOSM_RS10265"
                     /old_locus_tag="pgaptmp_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HGU4558571.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(32757..32843)
                     /gene="uof"
                     /locus_tag="ACHOSM_RS10270"
                     /old_locus_tag="pgaptmp_002054"
     CDS             complement(32757..32843)
                     /gene="uof"
                     /locus_tag="ACHOSM_RS10270"
                     /old_locus_tag="pgaptmp_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HGU4558572.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            complement(33053..33583)
                     /gene="fldA"
                     /locus_tag="ACHOSM_RS10275"
                     /old_locus_tag="pgaptmp_002055"
     CDS             complement(33053..33583)
                     /gene="fldA"
                     /locus_tag="ACHOSM_RS10275"
                     /old_locus_tag="pgaptmp_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HGU4558573.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            complement(33723..34016)
                     /gene="ybfE"
                     /locus_tag="ACHOSM_RS10280"
                     /old_locus_tag="pgaptmp_002056"
     CDS             complement(33723..34016)
                     /gene="ybfE"
                     /locus_tag="ACHOSM_RS10280"
                     /old_locus_tag="pgaptmp_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HGU4558574.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            complement(34156..34920)
                     /gene="ybfF"
                     /locus_tag="ACHOSM_RS10285"
                     /old_locus_tag="pgaptmp_002057"
     CDS             complement(34156..34920)
                     /gene="ybfF"
                     /locus_tag="ACHOSM_RS10285"
                     /old_locus_tag="pgaptmp_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4558575.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNLPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            35105..35650
                     /gene="seqA"
                     /locus_tag="ACHOSM_RS10290"
                     /old_locus_tag="pgaptmp_002058"
     CDS             35105..35650
                     /gene="seqA"
                     /locus_tag="ACHOSM_RS10290"
                     /old_locus_tag="pgaptmp_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HGU4558576.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            35676..37316
                     /gene="pgm"
                     /locus_tag="ACHOSM_RS10295"
                     /old_locus_tag="pgaptmp_002059"
     CDS             35676..37316
                     /gene="pgm"
                     /locus_tag="ACHOSM_RS10295"
                     /old_locus_tag="pgaptmp_002059"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HGU4558577.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(37372..38691)
                     /gene="potE"
                     /locus_tag="ACHOSM_RS10300"
                     /old_locus_tag="pgaptmp_002060"
     CDS             complement(37372..38691)
                     /gene="potE"
                     /locus_tag="ACHOSM_RS10300"
                     /old_locus_tag="pgaptmp_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HGU4558578.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(38688..40886)
                     /gene="speF"
                     /locus_tag="ACHOSM_RS10305"
                     /old_locus_tag="pgaptmp_002061"
     CDS             complement(38688..40886)
                     /gene="speF"
                     /locus_tag="ACHOSM_RS10305"
                     /old_locus_tag="pgaptmp_002061"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HGU4558579.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERIPAEYLSRISGVFEHGEARKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            complement(41196..41300)
                     /gene="speFL"
                     /locus_tag="ACHOSM_RS10310"
                     /old_locus_tag="pgaptmp_002062"
     CDS             complement(41196..41300)
                     /gene="speFL"
                     /locus_tag="ACHOSM_RS10310"
                     /old_locus_tag="pgaptmp_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HGU4558580.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            complement(41279..41413)
                     /locus_tag="ACHOSM_RS10315"
                     /old_locus_tag="pgaptmp_002063"
     CDS             complement(41279..41413)
                     /locus_tag="ACHOSM_RS10315"
                     /old_locus_tag="pgaptmp_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGU4558581.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
BASE COUNT        10244 a        10412 c        10571 g        10193 t
ORIGIN      
        1 tatttagtga atatttaagg ataaattatc atggacatgg agtcgcaaaa attactgttt
       61 gctctgtcaa ctccgatgga aatacgcaat gaatgctgtt taccttcgca ttcatcaccc
      121 aaaatgtatt taggtacacg cttttttgac ctttcttcct cctggggaat tgatgaccgt
      181 gatgacctgc tgcgcacaat tcaccgaatg atcgataacg gtcatgccgc tcggttggca
      241 gggttttatc accgctggtt tcgttattct ccatgtgaat ggcgtgacta tcttgctgaa
      301 ttaaatgagc aaggtcaggc ttatgcgcaa tttgtcgcca gcaccgccga atgctgtggc
      361 gaagggggaa tcaaagcctg ggactatgtc cgaatgggtt ttttaagccg aatgggcgta
      421 cttaataact ggttgagtga agaagaaagt ctatggatcc agtcgcgcat tcatctacgg
      481 gctctacgtt attatagtaa ctggcggcaa tactttgccg gttatacctt tggtcggcaa
      541 tattggcagt ctcccgaaga tgataatctg cctttattac gcgaattctt agcgcgtaaa
      601 gaatacgacg attccggcaa tgatatgttt tatcaattat ttgccagtga cgatgcatat
      661 tacgcgacct taccctggca accactggct gactacccca catgcccgga aacgcttaag
      721 gatatgagcg acctatgaaa aattgctgga agattctcga aatagaggaa acgactgacg
      781 tcgatattat ccgccgcgct tatctggcgc tgttatcgtc ctttcatcca gaaaccgatc
      841 cgcagggttt taaacaactt cgtcaggcgt atgaggatgc gctacggatt gcgcagtccc
      901 ctgctaaatc tgtttggcaa ccagaagaac atgaagttgc agaacatgaa attctgctcg
      961 cctttcgtgc gttactctcc tctgatagcg aacgttttct gccctctgcc tggcagcgat
     1021 tcattcagca attaaattat tgctcgatgg aagatattga tgaattacgc tggtcgctgt
     1081 gcacaatcgc catgaacact gcccatttat ccttcgagtg cgtggtgtta ttagcagaaa
     1141 gattgcggtg gttgcaggag gaaaacgtcg gggaaataga cgaagaagaa ctggaatcct
     1201 ttttatatgc cattgcgaag gggaatgttt ttaacttcca gaccattctg catctgcccg
     1261 ttgccgtaca aaatgacacc attgattttt accaaatgtt cgctcggatt tggtcatcgc
     1321 atccagaatg gctgacattg tatttagcgc aacatcgcgc agtgattatc cccgatgatg
     1381 caaatttgca cagaaattta ctccgctggt atagcgcagg tcgtcaggat atccccgaac
     1441 tagggcgtct ctactggcaa tcttctcagt gcgaagacaa cgcttcgcta cgcgaggcgc
     1501 taaccctgta caaatacgat agcgccggga gccggatgtt cgaagagctg gtggctggaa
     1561 gcgatcgttt ctatgccacg cttccctggc aaccattaac tgtccagcct gaatgcccgg
     1621 taacgcttaa ggatgtgagc gacctatgaa aacatgttgg caaatcctcg aaattgaaag
     1681 cacgacgcaa atagacatta tccgccaggc ttatcttgct cgcttaccgt tatgtcatcc
     1741 cgaaaccgat ccgcaggggt ttaaagcatt acgccaggcc tatgaagagg cgctgcgact
     1801 ggcggtaaat cctgtcgagg aagcagatga tgaagaaaaa gatgccaccg ctgaacatga
     1861 aatactacgt gcattcagga cattactgga ttcagaaagt gatcgttttc agtcctccgc
     1921 ctggcagaaa tttattcagc aattaaatac ctagaacatg gaggatgtcg atcaattacg
     1981 ctggccgctg tgtgcaatcg ctatagaagc gcgatatctg tcattaaatt gtgcttcttt
     2041 gttggcagag cgtttgaact ggcattcgtt taatgacagc gaaggaatgg atgaggaaga
     2101 aagggaggct tttcttgagg ccattcaggc tggtgattgt ttcgatttcc ttagccttct
     2161 ggaatatccc gtcgcgttac agaaccagac tgttgaatat tacttcgcgc tggaacgttg
     2221 ctgccgttac catcctgact atgtcactgc gtttttggcg atggaaggac cgtggttcat
     2281 tcctgatgat gcaaagttac atcgcaaact gttgcgctgg tacagctcgg tgcaaacagg
     2341 tatggtggaa ctcattcctg tagccaaaca gtggcaagcg gaagaaccag aaagcgaaga
     2401 tgcccggtat taccagtgtg cacaacgttt gtactgcggc gagggggaaa gcctgcttgc
     2461 cgatctcggc gcgtactggg aaagttaccc atctacacaa gctgataatt tgttgttgca
     2521 gtggagcaag cgtcattgcc cggattattt cgcgttatta gtgatggtta tcgaagcgcg
     2581 gagcatggta gatgcgcaag gtcaaccgct gaaatatgtt cctggtgaga gcgcccggac
     2641 gcggctgtta tgggcggaaa ttttacatag cggaaaatta tcgccgttag gtcaatcgtt
     2701 tattgagtcg ttattcttca aacgcaaagc atgggcgtgg tggaaatcga gagtcggtag
     2761 cgagacagaa gaagattcac cgttactgga tttgtatcgg gtagcggaac aggtagtact
     2821 tgaagcgttt ccgaaacaag agatgctggc ccgtcttaat acaaggctgg aaggcggaga
     2881 tgctcatcca ttagaggcca ttatcacccg gatgctgttg acgaaagtga aactcgagcc
     2941 ggaggatgaa gatgtcgatg agccaacacc tgaaaaccat gaagaaatac atgatgagga
     3001 tgaaaaacca cagagcatta ccagcattat caaaatcagt ttaacggtgc tggttatagg
     3061 ttatgctctc ggcaaaatcg cgatgttgtt tagttgacga tgtgtttttt gcgccgatgc
     3121 cggatgcagc atttacgccg catccggcag ccgtgattat gaggtctcaa tggctaaata
     3181 aacgctcgcc cgccgcctga cctcatcgac gcgggccata tcgtttgact gcaacgccgc
     3241 atcaaaatcg gtgatcaccc ggccaatctc ttctcgctcg tcacccagcg cccgcgccca
     3301 caactcttcc agtttggcct taaaggtgcg gttgatgagc atatcgcgcg gataaatttt
     3361 caatgcggat aaccgcgtcc gactctcttc aatctgctgc gcgctcaatg ttaccgggct
     3421 gtggttaatc actctggact taacgctgcc gtcttccaga agcacgtcaa cttcaagcaa
     3481 cccgttgata tcataactaa agcgaatatc aatcgactga taagccccgg ttttcttcaa
     3541 cggcacatcg aaggattcca ccagaatgtt gtttttaact ttgtggtttt ctccctgata
     3601 gacgttaacc gtaattgaat cctgttccgg gtgcatggtt gaataagttt ctacacgcga
     3661 cacgggcaca gtggtgtttc gttcaataat cggcgagaaa atgccggaaa cgccctggcg
     3721 gttaacttca acgcccaacg agtaagggca aatatcagtg aggatcacct cttcaatatc
     3781 ttcactgcgt aagcggcagg cggcctggat tgctgcgccc agcgcgacaa tggtgctcgg
     3841 atcgtaactt tgatacggta atttgccaaa cagacgcacg gcgattcgct gcaccagcgg
     3901 catttgtgac gcgccgccaa ccagcaccag actgtcgatt tgactcggct tcagacgtgc
     3961 atcgcgcaac gcctgttcaa tcggcactcg caagcgattg agcagcggca accacaaatc
     4021 ttccagttcg ttctcgtaaa attcgcactc ccgcgtttct tcctgatact gccagcgaat
     4081 gtgcaatggc gattgattgc tacattttgc cgcttccaca caggcgtaca aggctgccag
     4141 ttcactctcg ttaagcgtgg tcctggcgac atccgcgcgt tttaaaacct catcgaccag
     4201 catatgggta aaatcttcgc caccaagaaa gttgtcgcca gcggaggcgt gaacttcaat
     4261 caccggcgtg gcgtactcaa gcaccgtaac gtcaaacgtg ccgccaccga gatcaaacac
     4321 cagcgaacgg gtattttgtt gggtatgcag gccatacgcc atcgcagctg ctgtgggttc
     4381 attaattaag cgtaccgcat ttaacccggc taactccgct gctaaacggg tatgcttgcg
     4441 ttgttcatcg ctgaaataag ccggaacgga gatcaccaca tctttaatcg gacgttgcag
     4501 aaattcttcg gcatcttctt ttaatgagcg taataccaaa gaggacagtt ctggcgcgtt
     4561 aaatgtgtcg ctgcctaacc gccagttggt attactgccc attgcacgtt taaataacgc
     4621 tgccgtttta tccggatgcg aagtacgccg tgatacagcc ggttttccga ctaaaatatg
     4681 attattttca tccatgctaa ttatggatgg cgttaaatat tcaccaaact tatttggaat
     4741 taattgcgcg gcaccgtctt tccagacggc aattaaacta ttggtagtgc cgagatcgat
     4801 accaatggcg agttctgcat tatccattgc atttatatct tgaaagaaaa agagataaac
     4861 agattaagac cacccaggta acgcgtggtg atcttaatca atgacgtgtg ttaagcgtaa
     4921 aatttcagac gatcagccag taaatcaaca aagccctgac gatcaacatc gaccattaca
     4981 gtggcattcg gtttattgcc agtcagataa taataatcaa caaccgtcat accctggcta
     5041 tatttcccct gtgtttccac gccaacccag cgctcaacag tggtaaacaa ctcgggtttc
     5101 aacagccagg cgatggtgca tgggtcatgc agcggtgcac cgacaaagcc ccatttttcg
     5161 tctttatgat attcgaggaa gaaatccagc agttcggcaa caatggttga aacagggttg
     5221 ccaatcgcgc ggaaacgctc ggtgtcttca acgtggattt gtgctttgtg agtaacatcc
     5281 agagcggcca tcaccactgg gatccctgac tggaagacaa tttctgccgc ttccgggtca
     5341 acgtaaatat taaattcagc cgcaggcgtc cagttaccaa gccccattgc gccgcccata
     5401 atcacgatac gggcaatttt gctatgcagt tccggatggc tattgagcag caaggcaacg
     5461 ttagtttgcg gtccggtaga cacaatcgtg acaggttccg cactttcgcg cagcgttttc
     5521 gccatcagct ctaccgccgt acagttttgc ggtgcgaacg tcggttccgg taatgccggg
     5581 ccatccagac cgctttcgcc gtgcacattg tccgcgataa tcaactcacg cattaacggt
     5641 tttaccgcgc cgcctgctac cggaatatcg gtgcgattaa gcaaggtcag catacgcaga
     5701 acattgcgta aggttttttc tggtgtctgg tttccggcgg aagacgtaat tgctttgaca
     5761 tcaagctctg gtgaggcgag ggcgagaact attgcgatag cgtcgtcatg acctgggtcg
     5821 caatctaaca gaattggcag tgccattgtt gctccttgtt gtgtgcttct ttgcgacaag
     5881 ggtaacgcca ggatgtaaca gatacgaggg gcgaaacgat aaagcgtgag atggcgcgca
     5941 attgggtatg cgcgccagag tgattaatgc aggattttcg cgaggaagtc ttttgcgcgg
     6001 tccgatttcg gatcatcgaa gaaagcgtct ttcggcgagt cttcgacaat tttaccctca
     6061 tccataaaga tcacccgatt cgccactttg cgggcaaaac ccatttcgtg ggtcaccacc
     6121 atcatggtca ttccttcgtt tgccagttcc accatcacgt ccagcacttc gttgatcatc
     6181 tccggatcca gcgccgatgt cggttcgtcg aacagcatcg caataggatc catacacaac
     6241 gcgcgagcga ttgccacacg ctgctgctga ccgccggaaa gctgcgccgg aaatttattg
     6301 gcatgtgcgg aaagtcccac gcgctccagc aatttcaatg ctttttcacg agcgggcgct
     6361 ttatcgcgtt taagcacttt cacctgcgcc agggtcaggt tttcgataat cgacagatga
     6421 gggaacagct cgaaatgctg gaataccatc ccgacgcggg aacgcagctt tgccagatcg
     6481 gttttcttgt cattaatcac gataccatcg acggtgattt caccttgctg cactggttcg
     6541 aggccgttga cggttttaat cagcgttgat ttgccggaac cagacgggcc gcaaaccacc
     6601 accacttcgc cttttttcac ttcggttgag cagtcggtca gcacctgaaa gtgaccatac
     6661 cattttgaaa catttttcag ggtaatcatt atgctgtcct tcttttcaag tagctgacca
     6721 acaacgacgc gctaagacta ataacgaaat aaacaaatcc ggcaaacagg atcatctcaa
     6781 cctgcgtacc atcacgctca ccaatggttg aggcggtacg gaagaaatcg gccagagata
     6841 acacatacac cagtgaggta tcctggaaca gtacgatgcc ctgagtgagc agaagcggca
     6901 ccatcgcgcg gaacgcctgc ggcagaataa tcagtttcat cgactgccag tgagtcattc
     6961 ccaacgccag cgcggcgctc gactgaccac gagaaatact ttgaatacca gcacggataa
     7021 tctctgaata gtaggccgct tcaaacatcg aaaacgccac catcgccgaa attaaacgga
     7081 tatcattttt ttgtgataat cccagcacgt tttgcagaaa acctggcacg atcaggtaaa
     7141 accacagcaa aaccataact aaaggaatcg agcggaatac gttaacgtag gctttggcaa
     7201 accacgctac gggcgcaaag ctggacaagc gcatcaccgc cagcatggtg ccccacaaaa
     7261 taccaatcac gactgccgtg acggtgattt tcagcgtgat caccagcccg tcgagcagat
     7321 atggcaggga agggacaatg gaactccagt caaactcgta cattatttgc cccccatgtt
     7381 gccaggcagg cgaactttac gttcaaccag cgtcattacc agcatgataa ccgcgttaat
     7441 caacacatac gccagcgtaa tagcggtaaa cgactcccag gcgtgggctg agtaatcaag
     7501 caatttaccc gcctgcgccg ccatatccac cagaccaata gtcgaggcga tggcggagtt
     7561 tttcaccagg ttcatcatct cggaagtcat cggcgggacg ataacgcgat aagcattagg
     7621 cagcagtacg tatcgataag cctgcggtag cgtcaggccc atcgccagcg cggcattttt
     7681 ttgcccacgc gacaacgact gaatggcggc gcgtacctgt tcgcaaacac gggcggcggt
     7741 aaacagccct aggcagagca tggatgaaag gaaaaactga atattgggat ccagctcggc
     7801 cttaaaccac atgccgattt tctccggcaa cagctccggg atcaccagat accaggtaaa
     7861 gaattgcaca atcaacggca cgttgcggaa cagttcgaca tacaacgtac ccagaccgga
     7921 gagaaaacgg tttggaacgg tacgtaaaat gccgaaaaat gaaccgacga ggaaagcgat
     7981 aatccaggcg cagatcgaca aagcgatcgt cacctgaaaa ccactccaga tccaaccaag
     8041 ataggtggtg ttgccgaacg gggcttgttg taaaaaaata ccccagttcc agtctataga
     8101 cataaatcta ctccagaaaa aagagggtag cagcgttaac tgctaccctc gaagattgtt
     8161 acccagcgta gtgcggtttt caggcccgat ggggaacgac catcaggcgt atagtctgtc
     8221 cgtgctacgt aacaatcgag agggctggaa tttccgcccc tggttcttgt aattagttca
     8281 gtgccttgtc attcggttct ttgaacagtg ctttcatttc gtctgacagt tcgaaattca
     8341 tgttcaggtt tttcggcgga atgggatttt tgaaccattt atcaaaccat ttttccgctt
     8401 caccggaggt ctgcacctgc gcgatggtgt catccatcag ctttttgaac tgcggatcgt
     8461 ctttacgcaa catgcaaccg taggcttcct gagactgcgg cttgccgacg atttcccagt
     8521 tgtatggttt cttcgctttc gcacgttcac cggccagcag ggcatcatcc atcataaagg
     8581 caacggcacg accgctttct agggtgcgga aagagtcacc gtgatctttg gcgctgatga
     8641 tgcgcatatt cattttttgc tcttcattca gtttgttgag caaaacttca gaggtagtgc
     8701 cggaagtgac gactacggct ttgtctttca ggtcggcaaa atctttgata tcgccgccct
     8761 ttttggtcag caggcgcgta ccgaccacga aaatagtatc agagaaagcc gcctgttttt
     8821 ggcgttcgac gttgttggtg gtagaaccac attcaaaatc gaaagtgccg ttttgcagca
     8881 gtggaatacg gttttgtgag gtaatcggaa tcagttttac ctgcaagtcc ggtttgttga
     8941 gtttcttttt cactgcttca acaatggcgt tggagtaatc ctgcgagtaa cccaccactt
     9001 tttgctgatt gtcgtaataa gagaaaggta ctgaagattc acggtgaccg acgacaatca
     9061 caccgttttt ggcgattttg tccagcgtac tgcctgctgc cggggcggcg tcatctgcct
     9121 gtgccagtcc tgcggaaagc gccagggcga ggattgctgt ggcaggttta cgtaattgca
     9181 tatccaactc ctttatcttc tgcgttaaga acgcatggat acccgttgtg agtgtttgtg
     9241 ttgttatcgt ctgcaacttt attgtgcagt gttgtgcctg ttaggtaaca tttagtttgg
     9301 ctaaatgtaa agatattgct gttttattgt ttgtttttgc gagatgcgcc gcaccattcc
     9361 gaagcaaaat tcttaaaccg cacctttttg gtgctaccgc tgatttgtgg tggtgcaacc
     9421 tggttgcgtc gcggctgatt tcatcgctta cacagtaaat aaagcaactt acatgccaaa
     9481 aaatacttag gagtgaatta attacgattg taatttcagg ctgaaaaagt catatttcgg
     9541 acagagttat cttttcggct tgggacgttt gccgggatga taactgcaca cgggaaaaat
     9601 gctttttacc tgtctattca aggaagaatc accttatgtc tgcatcttcg gttaagccgt
     9661 taaatgttca attacccgca ataaccctta tcctttttgc gctctgtgtt gggatatttt
     9721 gttacctcgc acaatggatg agttatgaag aagtcgatca atccgcactc atccatctcg
     9781 gtgctaacgt tgcttctctc acgttgtcgg gtgaatcctg gcgcttattg agcagtgtct
     9841 ttctgcacaa cagtgtttct catttgctga tgaatatgtt tgcactcctg gtggtggggg
     9901 gagtggtgga acgtattctg gggaaatggc gactcctgat tgtctggcta ttctctggcg
     9961 tctttggtgg gctaatcagc gcctgttatg cgttacgcga aagtgagcag atagtcatca
    10021 gcgttggggc atccggggca attatgggaa tagctggcgc tgcgatagca acacagcttg
    10081 cttcaggtgc gggcacacac cataaaaacc agcggcgagt atttcctctg ttgggtatgg
    10141 tggcgctgac actgttgtac ggtgcccggc aaacaggaat agataacgct tgccacattg
    10201 gcggcctgat tgcgggtggc gcgttgggtt ggctgagcgc gcgtttatct gggcaaaacc
    10261 gactcgttac ggaaggtggg attattgttg cgggcagtct tcttctgacc ggggctatct
    10321 agcttgcgca gcagcagatg gatgagtcgg ttttacaggt caggcaaagc ctgcgtgaag
    10381 agttttatcc gcaggagatt gaacaagagc gacgacaaaa aaaacaacag ttagcggagg
    10441 aacgcaacgc cctcagggaa acattatccg ctccggtaag tcgtgaacag gccagtggtg
    10501 atttactcgc tgagattgcc gatatccatg atatggcgat cagtcgggat ggtaatacgt
    10561 tgtatgccgc aattgaaaac accaacagca ttgttgtttt cgacctcgga caaaagagaa
    10621 tcctgcatac ctttacagcg cccatagcga aagaaaagtc agtcaaacat tgtggtggct
    10681 gtaaagatca gggcgtcaga tcgctggcat taagcccgga tgaaaagttg ctttatgcga
    10741 cttcatttga agcgaatgcg ttatcggtca ttaacgtggc gacgggggag attattcagt
    10801 cgattaccac cggtgcacat cctgacagcc ttatcctctc gcgtgatggc acaaaagcct
    10861 gggtgatgaa tcgcaccagt aatagtgtgt cagcgattga tctggtgact tatcagcatg
    10921 tggaggatat cccgctggag aaatacgacg ggacggggac gagtaataaa cctggcgcct
    10981 gggttatggc actttccccg gatgaaaaaa cattgttgat acccggtatg gtcagaggtg
    11041 gcattgtacg catcaatacc atcacgcatc agaaagaaga ctttcccgca ggtgatgcgc
    11101 gtggaacgat atcggcgatg cgttttcgac ctgaaaacgg ggatgtaatt tttgccgaca
    11161 gccaggggat ttcacgtata agagctgagg atcaacaagc cagcattatg acgcaatggt
    11221 gtagcaggag cgtttattcc gttgaggata ttagcccgga cggtcagtat ttagcgttgg
    11281 tgtcatatgg cctgcaaggt tatgtcatcc tgctcaatat taatgccggg cagattattg
    11341 gcgtttatcc tgccagctac gttaatcacc ttcgttttac agcggatggt agaaaaatat
    11401 ttgttatggc gaagaacggg ttgatccaac tggacaggac gcgctcgctt gatccgcagg
    11461 caattattcg tcatccccaa tatggcaatg tggcttgtat ccctgaaccg taagaaaaag
    11521 gggcatgata accatgcccc aaagctcaat gagaaagtac gttttgctca tgcaggatac
    11581 atcaggcaca tcaacgttta cgtgctcgca gactcatcag cacagcggca aaaccaaaca
    11641 gcgccgtcag cacccacagc ggccagttgc cggtacgtgc atacggtgtg agtccggtgg
    11701 tcggtgtcac attagtggtc aacacctcgc gggtgaactg cgggatcatc gcctgaatct
    11761 caccctgcgg gccaatcacc gccgtaatgc cgttgttggt gctgcgcaac agtgggcgcg
    11821 ccagctccag agcacgcatt cgcgccatct ggaagtgctg ccatggacca atagacttac
    11881 caaaccatgc atcgttggag atagtcagca ggtagtcggt atccgggcgg aagttatcac
    11941 gcacttgctc gccgagaatg atctcgtagc aaatagccgc agtaagctca ataccatttg
    12001 ccgacagcgg cggctggata tatggcccac ggctgaacga tgacatcggc agatcaaaga
    12061 acggtgctaa cggacgcaga atcgactcca gcgggacaaa ctcgccaaac ggcaccagat
    12121 ggtttttgtt atagcgatcg gctgattcgt agctgtacgg cgcaccttta cccagcgtga
    12181 tgatggtgtt gtaggtatcg tagcggttct gcttattgag acgcgcgtca acaattccgg
    12241 ttacaagcgt actcccttta tcgcgcaact caccgtccag tgctttgagg aacggttgct
    12301 gattaatttc cagatcggtt atcgccgact ccggccagat aatcaacgat gatttgccca
    12361 tcagcggtgc cgttgcgttg tagtaaatct tcagcgtatt aagaagctgg ccttcgtccc
    12421 atttcagcga ttgcggaata tcgccctgaa ccatcgaaac ctgaatggtt ttctccggtt
    12481 gtggggtaaa ccactggatg taacgcagcg ggaagggaag ggcaaacagc acgacggcca
    12541 ccaccagcgg acgccagttg cgtttgacca acgccagtgc cagcaggcca ctaaccatca
    12601 tcagcaggaa gttaatggct tccacgccca ttatcggtgc cagccctttt aacgggccat
    12661 caatctggct atagccgaac tgtaaccacg ggaagcctgt cagtacccaa ccgcgcagaa
    12721 actcggtcac ttgccagaga gcaggggcgg caatcgccac gcgcagccag gtggttttcg
    12781 gccacagacg cgacagcacg ccagcaaaca gtccggtata cagcgacaaa tacgccgcca
    12841 gcagcaccac caggaagatg ttaaccgggc caggcattcc gccaaaggtc gcgatgctga
    12901 catagaccca gttaataccg ctgccaaaga ggccaaatcc ccagcaaaag ccaatagcgg
    12961 cagactggag tggacggcgt ttaacggtca gcgtctgaag ccccatcagc gaaataatcg
    13021 ccgcaggcca gacgtcgtaa ggagagaagg ccagcgttcc gcaggcaccg aataataacg
    13081 ccagcagcag gcgaatgcgc tggcgttcaa ttaatgaggc aaaagccatg tagttatcta
    13141 tccagtttcg gttttattca tccagcttcg gctggggtga gtcatccggg attttgacat
    13201 gaacctgaat aatacgccga ctgtcggcca tcgccacttt gaactggtaa ccgtcgatgt
    13261 cgatggtttc accacgcgcc ggaagatgcc caaacgcctg catcaccaga ccaccgatag
    13321 tgtcgacttc ttcatcgcta aagtgggtgc cgaacgcttc gttgaagtct tcaatggaag
    13381 ccagtgcgcg cacggtccag gtatgacgac tcagctgacg gaagtcgata tcatcttctt
    13441 cgtcatattc gtcttcaatc tcaccaacaa tcagttccag gatgtcttca atggtcacca
    13501 gaccggaaac cccaccgaat tcgtcaatga cgatcgccat gtggtaacgc tgagagcgaa
    13561 actctttcag catccggtct acgcgcttac tttcaggaac gacaaccgcc tgacgtaaca
    13621 ctttgtccat gctgaaggct tcagcatcgc tgcgcataaa cggcagcaag tctttcgcca
    13681 tcagaatccc ttcaatgtga tctttgtctt cgctaatcac cgggaaacgt gagtgggcgg
    13741 actcgatgat gacatcaagg cattcgtcca gcgtctggtt gcgtttcagg gtaatcatct
    13801 gggagcgggg gatcatgatg tcgcgaacgc gttggtctgc gatgtccatc accccttcga
    13861 gcatatcgcg cgtatcttcg tcgataaggt cgttctgccc ggaatcacgg atcagcgcca
    13921 gcagttcgtc acggtttttc ggttcaccgt ggaaaagttg gctgagtaac agggagaaaa
    13981 atcccttctt gttgcttatc gtgtcactac tgtgtgaatt gtcgtcgctc atggcgtcgt
    14041 atgggttctc atgttagtta atcaaaacgc cgtcgttaat caccaacggc ggggacgtct
    14101 gccagtcaaa tgcctggcaa attattcttt ctcggcaatg tacggatcct catagcccag
    14161 agcaagcata atctctgttt cgagggcttc catttcttct gcttcgtcat cttcgatgtg
    14221 atcgtaacct aacaaatgca gactgccgtg caccaccata tgcgcccagt gcgcctccag
    14281 tggtttgcct tgctcctgag cttccttctc aaccacctga cggcagataa ccagatcgcc
    14341 cagtagcgac atttccatgc caggcggcac ttcaaacggg aaggagagca cgttggtcgg
    14401 cttatcctta ccgcgatagg tcagattcag actgtggctt tcggcggtat cgaccacgcg
    14461 aatagtcact tccgattctt cctgaaactg cgggatcacc gcattcagcc atgtttgaaa
    14521 ctggctctct tccggtaacc cggaattatc ttcacatgcc agttgtaaat cgaggatcac
    14581 ctgactcatt tttgttcctg ttcttcgcgc ttgcgttctg ccgccagcgc cgcttttcgt
    14641 ttttgttcgg cttcttccca ggcttcatag gcgttaacga tacgcgccac cacagggtga
    14701 cgaaccacgt cttcgctgtg gaagaagtta aagctgatct cttcgacatc ggccagcact
    14761 tcgatggcgt gacgtaagcc tgatttagta ttacgcggca ggtcgatctg tgtgacgtcg
    14821 ccggtgataa ccgcttttga gttaaaaccg atacgggtca ggaacatctt catctgttcg
    14881 atggtggtgt tctggctctc atcgagaatg ataaacgcgt cgttcagcgt acgaccacgc
    14941 atataggcca gcggcgcgac ttcaataacg ttgcgctcaa tcagtttctc aactttctca
    15001 aagcccagca tttcaaacag cgcgtcgtac agtgggcgca gatacgggtc tactttctgg
    15061 cttaaatcgc caggcaggaa gcccagtttc tcaccggctt cgaccgccgg acgagtcagc
    15121 agaatacggc gaatctcctg acgctccagg gcatcaactg ccgcagccac tgccaggtag
    15181 gttttacccg tacccgccgg gccaacgccg aaggtaatgt catggtcgag aatattggcg
    15241 atgtactgcg cctggtttgg cgtgcgcggc ttaattacgc cgcgtttggt tttgatattg
    15301 accgctttgc cgtactccgg cacgctctcc gcactttgct ccagtactcg cgcttcttta
    15361 atcgcaaggt ggatctgttc cggttcgata tcctgaatct gaccgcgcat cggggctgta
    15421 tcgacataca ggctacgcag aatgtctgcc gcagcggtga cgcaaatcgg acggccggtc
    15481 agtttaaagt ggttgtcgcg gcgattgatc tcgatgccga gacggcgttc gagctgcttg
    15541 atgttgtcat caaacgggcc gcacaggctc aacagacgcg cgttgtctgc tggctccagg
    15601 gtgatttcgc gagtgtctat gttcaaaccg ttcctcttat ctgtatgccg ccggaagctg
    15661 aacattcacc ggcctataag gaaattattc acgccacagg aaaaaggcgc aagcattgcg
    15721 ataaagatgg ggataaagag agaaaaaaca aggcccaccg gaacggcggg cctgagaatt
    15781 acggctgata ataacccacg ccaaggtcgt tttctttgcg ggtacgggca atcaccgatt
    15841 ccggtgtttc tgccacgcgc agacccattt catcttcagt acgcaccact ttaccgcgca
    15901 gagagttcgg gtagacgtcg gtaatttcta catcgacgaa tttaccgatc atatccggtg
    15961 tgccctcgaa gttgaccacg cggttatttt cggtacgccc ggaaagctcc atgatgctct
    16021 tacgcgaagt cccttctacc agaatacgct gggtggtgcc gagcatccgg cggctccatg
    16081 ccatcgcttg ctgattgata cgttcttgca gaatatacag acgttgcttc ttctcttctt
    16141 ccggaacatc atcaaccata tcggcggctg gtgtacccgg acgtgcagag aagataaagc
    16201 tgtagctcat gtcgaaattg acgtcggcaa tcagcttcat cgttttctcg aagtcttcgg
    16261 tggtttcgcc agggaagcca acaatgaagt cagaactgat ctgaatatcc ggacgcgccg
    16321 cacgcagttt acggatgatc gctttgtact ccagcgccgt gtgagtacgg cccatcaggt
    16381 tcagaatgcg atcggaaccg ctttgtaccg gcagatgcag gaagctcacc agctccggcg
    16441 tgtcgcgata cacttcgatg atatcgtcgg tgaattcgat cggatggctg gtggtaaagc
    16501 gaatacgatc gatcccgtcg atcgcagcaa ccagacgcag cagatcggca aacgatccgg
    16561 tggagccgtc gtagttttca ccacgccagg cgttcacgtt ctgaccgagc aggttgactt
    16621 cacgcacgcc ctgagccgca agctgggcaa tctcaaacag aatatcgtca gacggacggc
    16681 ttacctcttc accacgggtg taaggcacca cgcagtaggt gcaatattta ttgcagcctt
    16741 ccatgatgga gacaaacgcg gtcggccctt cggcgcgcgg ttccggtaga cggtcaaact
    16801 tctcgatttc cgggaagctg atatctacaa ccgggctgcg gtcgccgcgc acggagttga
    16861 tcatctccgg cagacggtgc agcgtttgcg gcccaaaaat aatatcgaca tagtgggcgc
    16921 gctggcgaat gtgctcgcct tcttgcgatg ccacgcagcc accgacgccg ataatcaggt
    16981 ctggattctt ctcttttaac agtttccagc gacccaactg atggaagact ttttcctgag
    17041 ccttctcgcg gattgagcag gtgttcagca gcagcacatc cgcttcttcc gccacgtcgg
    17101 tcagttgata gccgtgggtg gcatccagca gatcggccat cttcgatgaa tcgtactcgt
    17161 tcatctgaca gccccaggtt ttaatatgga gttttttggt catcgacttg ctcttgcgaa
    17221 atagtagcca ggaatgcagg gcgcatagtg taatgctttg ctgccgttgt gaccagtatg
    17281 agcgttatca gcccttgggg gtaaaaatcc tgtaaactta aagcatattg ctaacaggat
    17341 gattgaccat gacaaatcaa ccaacggaaa ttgccattgt cggcggagga atggtcggcg
    17401 gcgcactggc gctggggctg gcacagcacg gattttcggt aacggtgatc gagcacgcag
    17461 aacctgcacc gtttgtcgct gatagccaac cggacgtgcg gatctcggcg atcagcgccg
    17521 catcggtatc attgcttaaa gggttagggg tctgggatgc agtacaggct atgcgttgcc
    17581 atccttaccg cagactggaa acgtgggagt gggaaacggc gcatgtgatg tttgacgccg
    17641 ctgaacttaa gctgccgctg cttggctata tggtggaaaa caccgtcctg caacaggcgc
    17701 tgtggcaggc gctggaagcg catccgaaag taacgttacg tgtgccaggc tcgctgattg
    17761 cattacatcg agataatgat cttcaggagc tggaactgaa gggcggtgaa acgatccgcg
    17821 cgaaactggt gattggtgcc gacggcgcaa attctcaggt gcggcagatg gcgggaattg
    17881 gcgttcatgc ctggcagtat gcgcagtcgt gcatgttgat tagcgtacag tgtgagaacg
    17941 atcccggcga cagcacctgg cagcaattta ccccggacgg accgcgtgcg tttctgccgt
    18001 tgtttgataa ctgggcatcg ctggtgtggt atgactctcc ggcgcgtatt cgccagttgc
    18061 agaatatgaa tatggcgcaa ctccaggcgg aaatcgcgaa gcatttcccg tcgcgtctgg
    18121 gttacgttac accgcttgcc gctggtgcgt ttccgctgac gcgccgacat gcgttgcagt
    18181 atgtgcagcc ggggcttgcg ctggtgggcg atgccgcgca taccatccat ccgctggcgg
    18241 ggcagggggt gaatcttggt tatcgtgatg tcgatgccct gattgatgtt ctggtgaatg
    18301 cccgcaacta cggtgaagcg tgggccagtt atcctgtcct caagcgttac cagatgcggc
    18361 gcatggcgga taacttcatt atgcaaagtg gtatggatct gttttatgcc ggattcagta
    18421 ataatctgcc gccgctgcgt tttgtgcgta atctcgggtt aatggcggca gagcgtgctg
    18481 gcgtgttgaa acgtcaggcg ctgaaatatg cgttagggtt gtagccttac aacattgccg
    18541 gatgcgtgcc aaccgtaggt cggataagac gcggcagcgt cgcatccgac attgaaggat
    18601 aagacgtgtc aacatcgcat tcgacattga atgaacgcag aaaagcaaaa agctcgccga
    18661 agcgagcttt tttaatgtgg ctggggtacg aggattcgaa cctcggaatg ccggaatcag
    18721 aatccggtgc cttaccgctt ggcgataccc caactgggtg cacttacgat gtaagcgtct
    18781 tgaataaatt ggctggggta cgaggattcg aacctcggaa tgccggaatc agaatccggt
    18841 gccttaccgc ttggcgatac cccaacaaat tggtttttga atttgccgaa cgtattcgat
    18901 acattcagaa tttggtggct acgacgggat tcgaacctgt gaccccatca ttatgagtga
    18961 tgtgctctaa ccaactgagc tacgtagcca gattgtttct tcgatggctg gggtacctgg
    19021 attcgaacca gggaatgccg gtatcaaaaa ccggtgcctt accgcttggc gataccccaa
    19081 taaccgggcg gtgaaccgct tactcgaaga agatggctgg ggtacctgga ttcgaaccag
    19141 ggaatgccgg tatcaaaaac cggtgcctta ccgcttggcg ataccccatc cgtacaacgc
    19201 tttctggtga atggtgcggg aggcgagact tgaactcgca caccttgcgg cgccagaacc
    19261 taaatctggt gcgtctacca atttcgccac tcccgcaaaa aagatggtgg ctacgacggg
    19321 attcgaacct gtgaccccat cattatgagt gatgtgctct aaccaactga gctacgtagc
    19381 catctttttt tcgcgatacc ttatcggcgt tgcggggcgc attatgcgta tagagccttg
    19441 cagcgtcaac ctctttttca aggaaaattg ctcgaaagtg actgtttggt taggttgcga
    19501 acagcatggc gctatattcg tcaattattg tttactttgt gttttttccc caccctacag
    19561 ccattcttct gtcgtacagg atgaaattcg gaattcaaca atagtggtgg gaaattaatc
    19621 tatgaaatac tggcctacag tgatgagttg tcaaacagtg atgtggcaaa cccggaacat
    19681 ttccttactg catatcagaa ttaacaagca cctcaataac tgaaacagcc ccggatttca
    19741 ccggggctgt ttcgcatttc ttacttatac gccgactggt gaacgccaac cgcacgacca
    19801 gacggatcgt ccattttctt gaacgcctca tcccactcaa tcgctttagc ggaagaacac
    19861 gcgacggaag gaccgcccgg tacgcactca gcggcgctcg gaagtgggaa cagttcttca
    19921 aagatctccc ggtacagata cgcttctttg gacgtcggcg tgttgtacgg gaagcggaag
    19981 cgggcagttt ccagctgttg atcagaaacc tgctgcgccg ccacttcttt cagggtgtcg
    20041 atccaactgt aaccgacgcc atcggagaac tgctctttct gccgccaggc cacgcttgca
    20101 ggcagatacg cttcaaaaca ttcacgcagg atgtgttttt ccattttgcc gttaccgcac
    20161 attttatcct gtgggttaat acgcatcgcc acatcaagga attttttgtc gaggaacgga
    20221 acgcgtgctt ccacgcccca ggctgacatc gctttgttgg cgcgcgcgca gtcatacata
    20281 tgcagggcca gcagtttacg caccgtctct tcatgcagtt ctttagcgtt gggcgcttta
    20341 tggaagtaaa gataaccgcc aaacacttca tcagaacctt caccggacag caccatttta
    20401 atgcccatcg ccttgatctt acgcgacatt aaatacatcg gtgttgaagc gcgaatagtg
    20461 gtcacatcat aagtttcgat gtggtaaatc acgtcgcgga tggcatccag accttcctgt
    20521 acagtgaagt gaatttcgtg atgcaccgtg cccagatggt ttgccacttc ctgggctgct
    20581 ttaagatccg gtgaacccgg cagacctaca gcaaaggagt gtaactgcgg ccaccaggct
    20641 tcagagcgtt cctgatcttc cacgcgacgg gctgcgtatt tcttggtgat agcggaaata
    20701 attgaggaat ccagaccacc agaaagcagc acaccgtaag gcacatcaga catcagatgg
    20761 cttttaacgg aatcttccag agcctgacgc agctcgtttt tgtcggtcac gttatctttc
    20821 actgcatcaa agtcgaacca gtcgcgatga taataagaac ggatttcgcc gtcctggctc
    20881 cacaaatagc tccccgccgg gaactcttta atcgtgcggc aaactggcac cagcgatttc
    20941 atttctgagg ccacatacag ctgaccgtgt tcgtcatagc ccatatacag tgggatgatc
    21001 cccagatggt cgcgaccaat caggtaggca tctttttcgc tgtcgtacag ggcaaaggca
    21061 aacatgccct gcaagtcgtc gagaaattcc ggcccttttt cctgatacag cgcgaggatc
    21121 acttcacagt cagaccctgt ctggaactga taacgatcgc catattcagc gcgcaatgcc
    21181 tggtggttgt agatttcacc gtttaccgcc agtacgtggg ttttttgttg gttgtagaga
    21241 ggttgcgccc ccgcgttaac gtcaacaatt gacagacgtt cgtgagcgag aatggcgtta
    21301 tcgctggcat aaataccgga ccagtccggg ccacgatgac gcatcaggcg tgacagctcg
    21361 agtgctttct tacgcagctc aactgcgtct gttttgatat cgaatacgcc aaaaattgaa
    21421 cacataacct tctccgttaa cctggtattt gttgcttgtt gtgtttgctt gtttaaaaaa
    21481 atgccgcaaa gcagcactgt gcgcaagcga tttggcggtg aaaaaaaaaa aacgtaatgg
    21541 tgattgtcga ttggtgaaaa aaggtctggt gtgaggatgt atttattgat gaatcgataa
    21601 tttttagcgg gttttattga atgtttatat tttacggggg ccaaattgct gacaaagtgc
    21661 aatttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag catgaaaatt
    21721 caatatattg caggagctgc gtaggcctga taagcgtagc gcatcaggca gtttggcgtt
    21781 tgtcatcaga gccaaccacg tccgcagacg tggttgctat tcagataacg tcgatttcag
    21841 cgaccgacgg gtaaatccag cttgggcgga acggcatact gtcgatatcg tcgagcgatg
    21901 aaacaccaga aagcaccaga atcgtttcca gacctgcctg gaagcccgcc agaatatcgg
    21961 tacgcaggtt atcgccgaca atcaccgttt cttccgaatg cgcctgcatt ttgtttaatg
    22021 ccgcgcggat gatccacgga ctcggtttac caacatagaa cggtttacgc ccggagattt
    22081 tttcgatgcc tgcacacaac gcgccacacg cgggataaaa accgcgcccg tgggtgtccg
    22141 gattggtggc gataaaacgt gcaccgttag cgacgaaata ggctgcttta tgcatcatgt
    22201 cccagttgta ggaacgcgtt tcgccaacaa tcacgaaatc agggttcaca tcggtaatag
    22261 tgaaaccggc tttgtacagt tcatgaatca gtgcgccttc gcccaccaca tacgctttct
    22321 tgccttcctg gcgacgcagg aaatcggcag tcgccatcgc agaggtataa aacacgctgt
    22381 caggtacatc gacacctgcg gtggcaaagc ggttcgccag atcttgccca gtctgcgaag
    22441 gatagttggt cagcaacacc agcggcaggc ctttatccat aatcccgtgc aaaaattccg
    22501 ctgcacccgg tacggcgacg ttatcgtgca tcagcacgcc gtcgatatcg caaattacat
    22561 ttttaatggt catggactac ccagaatatt gacaacaata agcgccacta taaaagcaca
    22621 ttaattttcc agcaaatgct ggagcaaaat accgttgagc atggcgcgtt ttaccagcgc
    22681 aaaagcgccg attgccgagc ggtgatccag ctcagaacgt accaccggca gattagtgcg
    22741 aaacgccttc agcgcctggg tattaatgca gctttcaata gcagggagca gcactttatc
    22801 ggcttcggtg atttcaccgg caataacaat tttttgcgga ttaaataagt tgatagcaat
    22861 ggcgatggtt ttacccagat gacgaccgac atactcaatt acttccgacg ccagactatc
    22921 gcctttgttc gcggctttgc agatagtttt gatggtgcag tcgtccagcg gcacgcggct
    22981 ctggtagccc tgctttaaca gattcaacac ccgttgttca atggcagcgt tggcggcgat
    23041 agtttccagg cagccaaagt tgccgcagtg gcagcgttca cccagcggtt cgacctgaat
    23101 atggccaatt tcaccgacgt taccgttgcg gccaataaaa atgcgcccgt tagagataat
    23161 cccggccccg gttccgcgat ggacacgcac cagaatggag tcttcgcaat cctgacttgc
    23221 accgaagtag tgctccgcca gcgccagact acggatatcg tgaccaacga aacaggtcac
    23281 tttaaaacgt tcttccagag cttctaccag cccccagttt tctacctgaa tatgcggcat
    23341 gtaatgaatt ttgccgctgt ccgggtcaac aagccctggc aggatcaccg aaatcgcgat
    23401 cagctcgcgc agtttgcgct ggtagctatc aataaactga gcaatggcat tcaacagggc
    23461 atgttccagc gtttgctggg tacgttccgg cagcgggtaa tgttcttctg ccagcacttt
    23521 gctgctgaga tcaaacaggg tgatggtggc gtcgtgacga ccaagccgta cgccgattgc
    23581 gtggaaattg cgggtttcgg tgacgatgga gatagcgcgg cggcccccgg tggaggcctg
    23641 ctgatcaact tctttgatca gcccgcgttc gataagctga cgcgtaattt tggttacgct
    23701 ggcgggggca agctggcttt gctcggcaat ctgaatccgc gagattggcc cgtactggtc
    23761 aatcaggcga taaaccgccg cgctgttaag ctgttttacg agatcaacat tacctatctg
    23821 agcttgtccg cctggtgtca tactttctct tattgagtta cgacctcgtt accgttaacg
    23881 atggtcttgg tgattttaaa atcaggtgtg aatgcggtca ggttggctac tttacctgcg
    23941 gcgagtgtgc cgagacgttt ctcaacgcca atcgcacgcg ccggatagag cgtcgccata
    24001 cgcagcactt catccagtgc gataccgcaa tgttcgacca gattacgcac gccttcaatc
    24061 atggttaagg atgaaccgct taacgtaccg ttctcatcca cacaaagtcc gttacggtag
    24121 tatattgttt tacccgcaaa aatgaactgt tcaatgttgg cacctgctgg cgcggtggcg
    24181 tcggtaacca gacacagttt gtcgccttta aggcgtttag cgttacgaat gttggcgtaa
    24241 tcaacatgca ggccatcagc gataataccg caataaatgt cagcttcgtc gaggatcgcg
    24301 cccgccaggc ccggttcacg accggtaata tacggcatcg cgttgtacag atgggtggca
    24361 aaggtaatcc ccgcgcggaa accggctttc gcttctttca acgtcgcgtt ggagtgaccg
    24421 gcagaaacca caatcccggc atttgccagt ttgctgatga cttccgcagg aaccatttcc
    24481 ggtgccaggg tcactttggt aatgacgtcg gcgttttcac acaggaaatc gaccagcgcg
    24541 gcatcaggct tacgcacaaa attcggatta tgggtgcctt tttttaccag attcagccac
    24601 ggaccttcca gatgcagacc taacgcctga ttcggatgtc ttgccaggta ctcgcgcata
    24661 acgcgcacgc cctgtttcat cagctcatcg ctggtggtga taagcgtcgg cagatagtta
    24721 gtacagcctg atttctcatt ggctttctgc atgatttcca gcgtttccac gctgaccgct
    24781 tcagcggtgt cgttaaactg tacgccgccg cagccgttta actgcacatc gataaaaccg
    24841 ggggagagaa tggccccgtt cagtgaacgt tgttcgatct ctggcggcag ttccgctacc
    24901 ggacagacgc ttttaatcag gccatcagcg ataacaaccg cgtggtcatc aagaaattcg
    24961 tggccggtaa agatccggcc ctgggttaat gcatacattc tgacccccga ttttaaaaaa
    25021 tattgccctg agcaaggagc cagggcaggg ataacaatta cagacctttg atattttctg
    25081 cttctaattc attgaaatat cttaaagtct taactttcag ctccatggtg gaaggttcat
    25141 cgcacaccat gatcgctttt ggatgcagtt gcagacagct gatggtccac atgtggttca
    25201 cgcaaccttc aactgcagcc tgcagtgcca gtgctttctg gctacccagc accagaatca
    25261 tcacttcttc ggcatccagc aatgtaccaa caccgacagt cagggcatat tttggcacct
    25321 gattgacatc gttatcaaag aaacgagagt tcgcgacgcg agtgtcatga gtcagggttt
    25381 tgatacgagt acgagaagcc agagaagacg ccggttcgtt aaatgcaata tggccgtcgt
    25441 tacctacacc gcccataaac agatgaattt ttccgtaaga acggattttt tcttcatact
    25501 ggcggcattc ggcgtcgata tccggggcgt tgccgttgag aaggttgatg ttttctgctg
    25561 gaatatcaac gtgatcgaag aaattacggt gcataaagct gtagtagctt tccggatgct
    25621 ctttcggcag accgacatat tcgtccatgt tgaaggtgac aacgtgctta aagctgacct
    25681 ggcctgcttt atgcatttcg actaacgctt tataggtggt catcggcgtg ccgccagttg
    25741 gcaggcccag aacaaacgga cgatcggcag tcggtttgaa cgcattgata cgattgacga
    25801 tatggcgagc agcccatttg ccgacctgtt cagcggtagt cagggggatc agtctcatta
    25861 ttcacctcaa taagtaaaat gtaagccgtt ggcggattag gcatctttaa gcgtaacctg
    25921 gatttgcgca gacaggcgtc aatccgacct gattttttga atgataaaat aagttttctg
    25981 gtttagccag taaaagggag tgatgataac gatatttggt gacaaaactc acaaaagaca
    26041 cgcgtttaat ttgcgatacg aattaaattt tcacacactc tgtagcagat gatctaacaa
    26101 tctgattaca gaacatcggc agtacaattt gcagcaaaat aaaaatacgg ctttaaacga
    26161 gccaaatagg gttctcgtag ggggaataag atgaatattt taggtttttt ccagcgactc
    26221 ggtagggcgt tacagctccc tatcgcggtg ctgccggtgg cggcactgtt gctgcgattc
    26281 ggtcagccag atttacttaa cgttgcgttt attgcccagg cgggcggtgc gatttttgat
    26341 aacctcgcat taatcttcgc catcggtgtg gcatccagct ggtcgaaaga cagcgcaggt
    26401 gcggcggcgc tggcgggtgc ggtaggttac tttgtgttaa ccaaagcgat ggtgaccatc
    26461 aacccagaaa taaacatggg tgtactggcg ggtatcatta ccggtctggt tggtggcgca
    26521 gcctataacc gttggtccga tattaaactg ccggacttcc tgagcttctt cggcggcaaa
    26581 cgctttgtgc cgattgccac cggcttcttc tgcctggtgc tggcggccat ttttggttac
    26641 gtctggccgc cggtacagca cgctatccat gcaggaggcg agtggatcgt ttctgcgggc
    26701 gcgctgggtt ccggtatctt tggtttcatc aaccgtctgc tgatcccaac cggtctgcat
    26761 caggtgctga acaccatcgc ctggttccag attggtgaat tcaccaacgc ggcgggtacg
    26821 gttttccacg gtgatatcaa ccgcttctat gccggtgacg gcaccgcggg gatgttcatg
    26881 tccggcttct tcccgattat gatgttcggt ctgccgggtg cggcgctggc gatgtacttc
    26941 gcagcaccga aagagcgtcg tccgatggtt ggcgggatgc tgctttctgt tgctgttact
    27001 gcgttcctga ccggtgtgac tgagccgctg gaattcctgt tcatgttcct tgcaccgctg
    27061 ctgtacctcc tgcacgcact gctgaccggt atcagcctgt ttgtggcaac actgctgggt
    27121 atccatgctg gcttctcttt ctctgcgggg gctatcgact acgcgttgat gtataacctg
    27181 ccggccgcca gccagaacgt ctggatgctg ctggttatgg gggttgtctt cttcgctatc
    27241 tacttcgtgg tgttcagttt ggttatccgc atgttcaacc tgaaaacgcc gggtcgtgaa
    27301 gataaagaag acgagatcgt tactgaagaa gccaacagca acactgaaga aggcctcaat
    27361 caactggcga ccaactatat tgctgcggtt ggcggcactg acaacctgaa agcaattgac
    27421 gcctgtatca cccgtctgcg cctgaccgtg gctgactctg cccgcgtcaa cgatacgatg
    27481 tgtaaacgtc tgggtgcttc tggtgtagtg aaactgaaca aacagactat tcaggtgatt
    27541 gttggcgcga aagcagaatc catcggcgat gcgatgaaga aagtcgttgc ccgtggtccg
    27601 gtagccgctg cgtcagctga agcaactccg gcaactgccg cgcctgtagc aaaaccgcag
    27661 gctgtaccaa acgcggtatc tatcgcggag ctggtatcgc cgattaccgg tgatgtcgtg
    27721 gcactggatc aggttcctga cgaagcattc gccagcaaag cggtgggtga cggtgtggcg
    27781 gtgaaaccga cagataaaat cgtcgtatca ccagccgcag ggacaatcgt gaaaatcttc
    27841 aacaccaacc acgcgttctg cctggaaacc gaaaaaggcg tggagatcgt cgtccatatg
    27901 ggtatcgaca ccgtagcgct ggaaggtaaa ggctttaaac gtctggtgga agagggtgcg
    27961 caggtaagcg cagggcaacc gattctggaa atggatctgg attacctgaa cgctaacgcc
    28021 cgctcgatga ttagcccggt ggtttgcagc aatatcgacg atttcagtgg cttgatcatt
    28081 aaagctcagg gccatgttgt ggcgggtcaa acaccgctgt atgaaatcaa aaagtaatct
    28141 gctttatgcc tgatgcgacg cttgagcgtc gcatccaaca atgacaagcg gtggagatct
    28201 tctctgccgc tttttttttt catcaatcat ccccataatc cttgttagat tatcaatttt
    28261 aaaaaactaa cagttgtcag cctgtcccgc ttataagatc atacgccgtt atacgttgtt
    28321 tacgctttga ggaatccacg atgagtgagg cagaagcccg cccgactaac tttatccgtc
    28381 agatcatcga tgaagatctg gccagtggta agcacaccac agtacacacc cgtttcccgc
    28441 cggagccgaa tggctatctg catattggcc atgcgaaatc tatctgcctg aacttcggga
    28501 tcgcccagga ctataaaggc cagtgcaacc tgcgtttcga cgacactaac ccggtaaaag
    28561 aagatatcga gtatgttgag tcgatcaaaa acgacgtaga gtggttaggt tttcactggt
    28621 ctggtaacgt ccgttactcc tccgattatt ttgatcagct ccacgcctat gcgatcgaac
    28681 tgatcaataa aggcctggcg tacgttgatg aactgacgcc ggaacagatc cgcgaatacc
    28741 gcggcaccct gacgcaaccg ggtaaaaaca gcccgtaccg cgaccgcagc gttgaagaga
    28801 acctggcgct gttcgaaaaa atgcgtgccg gtggttttga agaaggtaaa gcctgcctgc
    28861 gtgcgaaaat cgacatggct tcaccgttta tcgtgatgcg cgatccggtg ctgtaccgca
    28921 ttaaatttgc tgaacaccac cagactggca acaagtggtg catctacccg atgtacgact
    28981 tcacccactg catcagcgat gccctggaag gtattacgca ctctctgtgt acgcttgagt
    29041 tccaggacaa ccgtcgcctg tacgactggg tgctggacaa catcaccatt cctgttcacc
    29101 cgcgccagta cgaattctcg cgcctgaatc tggaatacac tgtgatgtcc aagcgtaagc
    29161 tgaacctgct ggtgaccgac aagcacgttg aaggctggga tgacccgcgt atgccgacca
    29221 tttccggtct gcgtcgtcgt ggttacactg ctgcttctat tcgtgagttc tgcaaacgca
    29281 tcggcgtgac caagcaggac aacaccattg agatggcgtc gctggaatcc tgcatccgtg
    29341 aagatctcaa cgaaaacgcc ccgcgcgcaa tggcggttat cgatccggtg aaactggtta
    29401 tcgaaaacta tcagggcgaa ggcgaaatgg ttaccatgcc gaaccatccg aacaaaccgg
    29461 aaatgggtag ccgccaggtg ccgtttagcg gtgagatttg gatcgatcgc gccgatttcc
    29521 gcgaagaagc taacaagcag tacaaacgtc tggtgctggg taaagaagtg cgtctgcgta
    29581 atgcttacgt cattaaggca gaacgcgtgg agaaagatgc cgaaggcaat atcaccacca
    29641 tcttctgtac ttatgacgcc gacaccttaa gcaaagatcc ggcagatggt cgtaaagtca
    29701 aaggtgttat tcactgggtg agcgcggcac atgcgctgcc ggttgaaatc cgtctgtacg
    29761 accggctgtt cagcgtgcct aacccaggtg ctgcggatga tttcctgtcg gtgattaacc
    29821 cggaatcgct ggtgatcaaa cagggtttcg ctgaaccgtc tctgaaagac gcggtagcgg
    29881 gtaaagcatt ccagtttgag cgagaaggtt acttctgcct cgacagccgc cattctacgg
    29941 cggaaaaacc ggtatttaac cgcaccgttg ggctgcgtga tacctgggcg aaagtaggcg
    30001 agtaatttta agttctacca ggccgggtga ggcatttacg ccgcatccgg caagaaatag
    30061 caaacaatcc caaacgccgc gctctgcggc gttttttctg cttttcttcg cgaattaatt
    30121 ccgcttcgca atttatccat aaaataaatt taaaatcaca aaacataatt aaataaaatg
    30181 taaccgcttt catcttgctg gaatttcacg cttttattct tctgcaagcc tttcaaccgc
    30241 aaacttaagc cttgtaacaa aaatcatcaa aatatgtgcg gttgctcatg ttcttacatt
    30301 ctggttacag aaagagattg ataattcgcg tcgcgaaaaa tagtctgttc ctgtagtcag
    30361 cgagactttt ctcaacgcta cttttttaat ttttattttt tcgctgttca cctttggtgc
    30421 agcaatttat atgtcaaaga ggattaaccc atgcgtacgt ttagtggcaa acgtagtacg
    30481 ctggcgctgg ctatcgccgg tgttacagca atgtcgggct ttatggcaat gccggaggct
    30541 cgcgccgaag gattcatcga cgattcaacc ttaaccggcg gtatatatta ctggcagcgt
    30601 gaacgcgacc gtaaagatgt taccgacggc gacaaataca aaaccaacct ttctcactcc
    30661 acctggaatg ccaacctcga ttttcagtcc ggttatgctg ctgatatgtt cggccttgat
    30721 attgctgcgt ttacggcgat tgaaatggcg gaaaacggcg acagctccca cccgaacgaa
    30781 atcgcgtttt caaaaagtaa taaagcctat gacgaagact ggtccggcga caaaagcggt
    30841 ataagcctgt ataaagctgc ggccaaattt aaatacggtc cggtttgggc gagggcaggt
    30901 tacattcagc caactggtca aacgctgtta gcgccgcact ggagctttat gccaggtact
    30961 tatcaggggg cggaagccgg ggcgaatttt gattacggcg atgctggtgc gttgagtttc
    31021 tcctacatgt ggaccaacga atacaaagcg ccgtggcatc tggaaatgga tgagttttat
    31081 cagaacgata aaaccaccaa agttgattat ctgcactccc ttggggcgaa atacgacttc
    31141 aaaaataact tcgtactgga agcggcattt ggtcaggcgg aagggtatat cgatcaatat
    31201 tttgccaaag ccagctacaa atttgatatc gccggtagcc cgttaaccac cagctaccag
    31261 ttctatggta cgcgcgataa agttgacgat cgcagcgtca acgaccttta tgacggcacc
    31321 gcctggctgc aagcgttgac ctttggttac cgggcggctg acgtagtgga tttgcgcctc
    31381 gaaggcacct gggttaaggc tgacggtcag cagggatact tcctgcaacg tatgactcca
    31441 acctacgctt cctccaacgg ccgcctggat atctggtggg ataaccgttc cgacttcaac
    31501 gctaacggcg aaaaagcggt cttcttcggt gcgatgtatg acctgaaaaa ctggaatctt
    31561 ccaggcttcg ccatcggcgc ttcctacgtt tacgcatggg atgctaaacc tgcgacctgg
    31621 cagagcaatc cggatgcgta ctacgacaaa aaccggacta ttgaagagtc tgcatacagc
    31681 ctggatgcgg tctacaccat tcaggacggt cgcgccaaag gcacgatgtt caaactgcac
    31741 ttcaccgaat acgacaacca ctccgacatc ccaagctggg gcggtggtta cggcaacatc
    31801 ttccaggatg agcgtgacgt gaaatttatg gtaatcgcac cattcaccat cttctgatgc
    31861 ccgacgcggc aggttttgtg cctgccgcac gtttgaggaa tttgctatga aaaaactgat
    31921 tctcatcgcc ataatggcat cggggctggt agcttgtgcg caatcaaccg cgccacagga
    31981 agacagtcgt ctgaaagagg catacagcgc ctgtatcaac accgcacaag gttcgccaga
    32041 aaaaattgaa gcctgccaga gcgtgttaaa cgtgctgaag aaagagaaac aacatcagca
    32101 ttttgccgac caggaaagtg tccgcgtact ggattatcag cagtgtctgc gtgcaacgca
    32161 aaccggaaat gatcaggcgg tgaaagccga ttgtgataaa gtttggcagg aaattcgcag
    32221 taataacaaa taagtgagag ctgtaactct cgcttttctt atttcccttg cataaaaaag
    32281 ccaacccgca ggttggcttt ttctcattca ggctggctta tttgccttcg tgcgcatgtt
    32341 catcttcgcg gcaatcgcct tcggcacagt gaccgtaaag atagagactg tggttagtca
    32401 ggcgaatgcc atgttttgcg gcaatttcac gctgacgcgc ttcgatggaa tcatcactaa
    32461 attcgataac cttgccgcag tcgaggcaga tcaggtgatc gtggtgatgt tgctgtgtca
    32521 gttcaaatac ggatttaccg ccttcaaaat tgtggcgggt gacgatacca gcgtcgtcaa
    32581 actggttcag tacgcgatat accgtagcca gaccaatttc ttcacccata tcgatcagac
    32641 gtttgtataa atcttccgca ctgacgtgat ggttgtccgg ctcctgaaga acttccagga
    32701 tttttaaacg aggaagcgtt actttcaggc cagctttctt tagggcggta ttgttatcag
    32761 tcatgcggaa tctgtcctgt tactaagcgg ttcacttcat tagaagaagt gacagaattt
    32821 gctcttgaga taatgcgtat cattatagaa ttgccacgcc taaatgaaaa ccacaagtcc
    32881 ctggcaaata ttgttaataa aaacgtggca cagcttacat ttacaacggc aaggccactt
    32941 gaacaccggg acattgtaca ggtacaacag caaaagttac aaatttgtag caattatttt
    33001 gattggcatt atctattaat gcggcgtaga catgagtcta cgccgcatca catcaggcat
    33061 tgagaatttc gtcgagatgc aactcttcag aaatctgttt aacccatttt tctacacgtt
    33121 cagcggtcag ttccggctga cggtcttcgt cgatagccag accgacaaag tggtcgtcat
    33181 cagccagacc ttttgatgct tcgaaatgat agcccgcagt tggccagtga ccaacgatcg
    33241 ttgcaccgcg cggttcaatg atgtcgcgga tggtgcccaa tgcgtcgcag aaatattcgg
    33301 cgtaatcttc ctggtcacca caaccaaaca gcgcaaccag tttgccgttg aaatcaatct
    33361 cttcgagagt cgggaagaag tcatcccagt cacactgcgc ttcgccataa taccaggttg
    33421 ggatgcccag cagcagaatg tcataagctt ccagatcttc tttgctgctt tttgcaatgt
    33481 catggacatc ggcaacgtct ttaccaagct gtttttgaat catttttgcg atattttcgg
    33541 tattaccggt gtcgctgccg aaaaagatgc cagtgatagc catgagtgaa ataacctctt
    33601 gaaacttatt gaaatggggg tggaaaattg cccacggata aaggcaatca tagcagaaca
    33661 ggcagtcttg cggaatcagc aaacgagcag gactgcacac tgtgctacat gaaagtggaa
    33721 atttaaacga tgccctgact acgcagcgcc gccagttgct gcattaacat ctcttcgatc
    33781 agttcgctac ggctcatatt gcgcgactcc gccagctcgt tcagcgcctc gacagcttcc
    33841 gcgttcagct tcagttcgac acgcttaagg ccacgtactt tgtcgcgttt tagctggttg
    33901 cgtttattaa tacgcagctg ttcatcgcgc gaaagcggat tagttttcgg tcgtcccggt
    33961 cgacgctcgt gcgcgaacag atctaatgtc gtacggtccg tttgttcttt ggccatgatc
    34021 ttggtgactt cgggggaaac aatcagccag gcctctgccc ggatggatag cgcgccataa
    34081 tacatcagcg cgatgagtca cgccaacgcc cacgcgcgga aagcgacgcg gacgctgggt
    34141 ttttaatcag ttgcgttaat cattgagata gcgacggata gcgcgtaata ccgcatccgg
    34201 tttttcagca tggacccagt gacccgcgcc tgcaatcaca tgcgcccgtg cctgtggaaa
    34261 ttgtgccagt aaatcatcac ggtactgctc gctaacatac ggagaattgc cgccagggat
    34321 aaacagggca gggtgatccc atgccgggat tttctcccaa cccacaatat gcggatactg
    34381 atcccacaat accggcaagt taaagcgcca ctccccgtca acaaaagatt tcaacagaaa
    34441 ctgaatcacc ccttcttcat taagatgctg gcgcattatt gctgctgctt gctggcgagt
    34501 ttgtgcgtcc gattcactga ccgcgttgat agccgcgaaa atctcatcat gacggcgtac
    34561 gtgatagtcg accggtgcga tatcgatcgc caccagttta tcgatgcgat cgcgggccag
    34621 tgccgtaagt gccattaccg ctttaccgcc catggagtga ccgataaatg ttgctttgtc
    34681 gatctgctgt gcatccaggg tatcaacaag atcctgcgcc atcgccgggt aattcattac
    34741 cggatctctc ggtgaaagac cgtggttacg catatcaacc tggatgatat tgtgatcgtt
    34801 taccagatcg cgagccagta cgccaaggtt gtcgaggcta ccaaacagac catggacaag
    34861 aacgatggga gaattattgt gctggttttg tgcagtttgc gcgcggatat tcaatttcat
    34921 ggcaaagttc tttttttcgc gttgtcgggt tagggtatta tgttgaccat tgtgccacag
    34981 ggctgcaaca aataaggttt attccgagtt tttctgcaag ccaggcttga cgctatccgc
    35041 tgccgggatt tattcatata ctcctggcga cttgtattca gctaagacac tgcactggat
    35101 taagatgaaa acgattgaag ttgatgatga actctacagc tatattgcca gccacactaa
    35161 gcatatcggc gagagcgcat ccgacatttt acggcgtatg ttgaaatttt ccgccgcatc
    35221 acagcctgct gctccggtga cgaaagaggt tcgcgttgcg tcacctgcta tcgtcgaagc
    35281 gaagccggtc aaaacgatta aagacaaggt tcgcgcaatg cgtgaacttc tgctttcgga
    35341 cgaatacgca gagcaaaagc gagcggtcaa tcgctttatg ctgctgttgt ctacactata
    35401 ttctcttgac gcccaggcgt ttgccgaagc aacggaatcg ttgcacggtc gtacacgcgt
    35461 ttactttgcg gcagatgaac aaacgctgct gaaaaatggt aatcagacca agccgaaaca
    35521 tgtgccaggc acgccgtatt gggtgatcac caacaccaac accggccgta aatgcagcat
    35581 gatcgaacac atcatgcagt cgatgcaatt cccggcggaa ttgattgaga aggtttgcgg
    35641 aactatctaa aacgttgcag acaaaggaca aagcaatggc aatccacaat cgtgcaggcc
    35701 aacctgcaca acagagtgat ttgattaacg tcgcccaact gacggcgcaa tattatgtac
    35761 tgaaaccaga agcagggaat gcggagcacg cggtgaaatt cggtacttcc ggtcaccgtg
    35821 gcagtgcagc gcgccacagc tttaacgagc cgcacattct ggcgatcgct caggcaattg
    35881 ctgaagaacg tgcgaaaaac ggcatcactg gcccttgcta tgtgggtaaa gatactcacg
    35941 ccctgtccga gcctgcgttc atttccgtac tggaagtgct ggcagcgaac ggcgttgatg
    36001 tcattgtgca ggaaaacaat ggcttcactc caacgcctgc cgtttccaat gccatcctgg
    36061 ttcacaataa aaaaggtggc ccgctggcag acggtatcgt gattacaccg tcccataacc
    36121 cgccggaaga tggtggtatc aagtacaatc cgccaaatgg tggcccggct gataccaacg
    36181 tcaccaaagt ggtggaagac agggccaacg cactgctggc cgatggcctg aaaggcgtga
    36241 agcgtatctc cctcgacgaa gcgatggcat ccggtcatgt gaaagagcag gatctggtgc
    36301 agccgtttgt ggaaggtctg gccgatatcg ttgatatggc ggcgattcag aaagcgggcc
    36361 tgacgctggg cgttgatccg ctgggcggtt ccggtatcga atactggaag cgtattggcg
    36421 agtattacaa cctcaacctg accattgtta acgatcaggt cgatcaaacc ttccgcttta
    36481 tgcaccttga taaagacggc gcgatccgta tggactgctc ctccgagtgt gcgatggcgg
    36541 gcctgctggc actgcgtgat aagttcgatc tggcgtttgc taacgacccg gattatgacc
    36601 gtcacggtat cgtcactccg gcaggtttga tgaatccgaa ccactacctg gcggtggcga
    36661 tcaactacct gttccagcat cgtccgcagt ggggcaaaga tgttgctgtc ggtaaaacgc
    36721 tggtttcttc tgcgatgatc gaccgtgtgg tcaatgactt gggccgcaag ctggtagaag
    36781 tcccggtagg tttcaaatgg tttgttgatg gtctgttcga cggcagcttc ggctttggcg
    36841 gcgaagagag cgcaggggct tccttcctgc gtttcgacgg cacgccgtgg tccaccgaca
    36901 aagacggcat catcatgtgt ctgctggcgg cggaaatcac cgctgtcacc ggtaagaacc
    36961 cgcaggaaca ctacaacgaa ctggcaaaac gctttggtgc gccgagctac aaccgtttgc
    37021 aggcagctgc gacttccgca caaaaagcgg cgctgtctaa gctgtctccg gaaatggtga
    37081 gcgccagcac cctggcaggc gacccgatca ccgcgcgcct gacggcggca ccgggcaatg
    37141 gtgcttctat cggcggtctg aaagtgatga ctgacaacgg ctggttcgcc gcgcgtccgt
    37201 caggcacgga agacgcatat aagatctact gcgaaagctt cctcggtgaa gaacatcgca
    37261 agcagattga gaaagaagcg gttgagattg ttagcgaagt tctgaaaaac gcgtaaacac
    37321 atttaataaa aaaagggcgg tcgcaagatc gccctttttt actttgcttt tttaaccgtg
    37381 tttatttttc agttcaaagc gtggtgatac cagaccatac agtgtccaac caaggaaggt
    37441 cacgatggaa ccgtacagca tggcttcttc cccggatgag tacagcgcat aaaagctata
    37501 catcgcgcca acaaaagcaa caaagtttgc aactttcgct tttgaggggg gcacattagc
    37561 gaccttctga ataatgacta atgccgccat tgacagaata tacggaatga tattggtcac
    37621 cacggccagg ttaaccagca cgttgaactg actgtttagc gacgggctaa tcgtcatcag
    37681 cgccaacccg ctctggataa tcacaatggt caacattccc tgcaccggtg catccacttt
    37741 ggttacacgg gagaaaattt tagggaagta gccttcatca gatgaagatt taaacacctg
    37801 ggcaatggtg aactgccagc caagcagcga accgcagcag gacatcacca tcagcgccat
    37861 aatgacttta cccacttctg gtgtgaacat ctgtgcgaag gccaggccaa atggtgccgt
    37921 tgaatttgcc agctccatat taggcacaat ccctgcaatc acgttggtgg agacgatata
    37981 aatcaccgcc gcacctaacg taccgccgag taccgcgatt ggcacattac gttccgggtt
    38041 ttccactaca tcagtattcg cacacgcaga ctccagacca agaaaagccc acagcgtcat
    38101 agcgatggaa gaacctactg cactgaagaa cggtgcatga tgcggattcc aggaatcaac
    38161 gtaaagcgtc gggctaaacc agaaccagcc gataatgcac aggcctacca ccggaataat
    38221 gaccccccac acggtaatgc tactgatttg cccggtaatg cgcgcaccac caaagttagc
    38281 cacggtacaa atccacagca ccccgatggt cgcaagacca atctgcactg gcgacaaact
    38341 cgcgccgagc agttcggtgc cgtaaccaac cgccgaaata gcaatcgcga cgttagcaat
    38401 cagcaatgag acgccgtagg tatagttcgc cataaagtta ccggatttac cgaaagcata
    38461 ctcggcataa ccgcccatac cacctgattt acggctgaac ataccgcatt ttgcgaatgc
    38521 ccatgccagt gccattgagc ctacggctgt caccagccag gagataattg aaattgtccc
    38581 aacttcggca agctttgtcg gcagcatgat gataccggag cccatcatgt tgaccatcgt
    38641 cagtatggtt aactgaacga cgcccatttt gttcgattta gcctgactca taatttttcc
    38701 cctttcaaca gggtgctttg cgcatcacga ggcttgatga catagcacca aacttgctta
    38761 cgaccatcat gttcttcgat atagacaccc tgcagctctg gtgcaaaacc tggcagcagg
    38821 ttgatccctt cttccagagc gctgaagtaa cgcagaacag caccacccca gatttcaccc
    38881 ggaacaacac acagcacacc cggaggataa ggaagcgcac cttcggcagc gatacggcct
    38941 tctgcatccg gcagacgaac cagctccact tcaccgcgta aataggcgta gttggcttct
    39001 tgtggattca tgcttacgcg tgggaagtgt tccttacgga acatctcttt ttgcagttgt
    39061 ttcacgttgt gacgggcata caaatcatgc atttcctgac acaactgacg cagggtataa
    39121 ccggcgtagc gctcttcatg ctgtttgtag atggaaggca gcacttctgc taatggcgcg
    39181 tcggactcaa gcagtttttc gaagcgtacc agcagggcaa caagttgctg aagtttggcc
    39241 atatcttctg ccggagtcag caggaagagg atggagttaa gatcgcattt ttccggcact
    39301 acgccatttt cgcgcaggaa gttagcaaga atcgtcgcgg gtacaccgaa cgcttcatat
    39361 tcgccgttac gtgcatcaat acctggggtt gtcagcaaca gtttgcaagg atcgacaaag
    39421 tattggttct ctgcgtagcc ttcaaaagaa tgccagtgct cacctggtac aaactggaag
    39481 aagcgcagat caaccgcaat ttgcgctgtt tcatacgact gccagggttt accatccacc
    39541 agttcaggta cgaacggacg aatatgctga cagttatcga ggatcagctt gcgggcatta
    39601 atgccattta ccacacaatc catccacata ttacgaccgc tgacaccttc atgcattttg
    39661 gcgttgatat ccagtgcggc aaacagcgga tagaacgggc tggtggaggc atgcatcata
    39721 aaggcgttgt tcatgcgttt gtgcggtaca taacgctgtt gccctttgat gtggctgtct
    39781 tttttatgga tttgtgaagt ttgagaaaaa ccagcctgct gtttatgtac ggattgcgta
    39841 accagaatac ccggatcgtt ctcattaaga tccagcaaca gcggcgaaca gtcagccatc
    39901 atcgggataa attgctcata gccaacccat gcggagtcaa acagaatgta gtcacacaga
    39961 tgaccaattt tatccaccac ctggcgggcg ttataaatcg taccatcgta ggtgcctaac
    40021 tgaatcacag cgaggcggaa aggacgagcc tcttttgccc gctgcggtgc gacttccgcg
    40081 atcagctcac gcaggtaact ttcttcaaaa cagtgcgcat caatgccacc gataaagcca
    40141 tacgggttac gtgccgtttc cagataaacc ggtgttgcac cagcctgtag caacgctccg
    40201 tggtggttcg atttgtggtt attgcggtca aacagcacca gatcacccgg tgttagcagg
    40261 gcgtttaaaa ccactttgtt agaagatgaa gtgccattta aaacgaagta ggttttatcg
    40321 gcattaaaca cttttgctgc atgttgctgt gcaatgcatg gcgcgccttc gtgaatcagc
    40381 agatcgccca tcgctacgtc ggcgttgcac aagtcggcac ggaacagcgc ctcaccaaag
    40441 tattccacaa actgatttcc cgccggatgg cgacggaaaa attcgccgcc ctgatgaccg
    40501 gggcagtcaa atgcgctgtt accttgattg acataatcga ccagtgcgcg gaagaaaggt
    40561 gggcgcagtt gagtttcata atggctggca gcggtttcta actgacgacc ataaaattct
    40621 ttacgtgctt cgccatgttc gaagacacca gaaatacgtg aaagatattc tgctggtata
    40681 cgttcttcat tttccgttgc aataaaaaca ggaatgtcat agccagtagc gtctatttca
    40741 tcgagttttc cacgttcaac atcactgact gataaaataa tggcggcaac gtcaatataa
    40801 ttactttcgt ctatattcac gcatgcacga tgagtggaaa aacaatccgg gcaagaacga
    40861 ctaacggcaa tttttaattc tgacattttg ttcatctctt tatttcaggt aatagcaggt
    40921 cctcaatttc tcaaaggaga aatcgatcac gccggaaaaa agcaaagctc caccgctgat
    40981 aagtaaatca gtagtttgct ttttgcagat gcaataaccc agaccagccc ggtaactggc
    41041 tatactcgtc atacttcaag ttgcatatgc tgcgactcga attatgtaga gtttaaaagg
    41101 gttagcagta tatgagcacc gagggataca ggtattaacc tgtaatgctt aaagtcaggg
    41161 agacctgtac gctggggtgc cctttagtca gaagactagc gggcattaaa gaaatgaaag
    41221 tcaaaactat tacgatgggc aaacttcatg atatgggttg tccgccttat atgaggcatt
    41281 aagcggttat tattttccat ttacctttac ctcttgctgt gtcagttgaa ggtagagtta
    41341 ttctatccat gaaatggcta taatctgtga agaagatcaa caaaaaacga tcatacccca
    41401 gataataaat cattaattat
//
LOCUS       DAZKHC020000033        40840 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZKHC020000033 DAZKHC020000000
VERSION     DAZKHC020000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 40840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.033"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1938
                     /gene="yegI"
                     /locus_tag="ACHOSM_RS10320"
                     /old_locus_tag="pgaptmp_002064"
     CDS             <1..1938
                     /gene="yegI"
                     /locus_tag="ACHOSM_RS10320"
                     /old_locus_tag="pgaptmp_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HGU4558582.1"
                     /translation="NMKVFTSTGELTTLGRELGKGGEGAVYDINEFVDSVAKIYHTPP
                     PALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYSP
                     AHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDSD
                     SFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGRH
                     PYSGVPLISDTGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQA
                     FTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIYF
                     IDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYII
                     LLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFNRAK
                     MDYDHLVSQSQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQK
                     FLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWKA
                     SCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMPL
                     MEPLRQAAEKLAQAQADLSRC"
     gene            complement(1951..3303)
                     /gene="yegD"
                     /locus_tag="ACHOSM_RS10325"
                     /old_locus_tag="pgaptmp_002065"
     CDS             complement(1951..3303)
                     /gene="yegD"
                     /locus_tag="ACHOSM_RS10325"
                     /old_locus_tag="pgaptmp_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4558583.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQVQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            3437..4294
                     /gene="alkA"
                     /locus_tag="ACHOSM_RS10330"
                     /old_locus_tag="pgaptmp_002066"
     CDS             3437..4294
                     /gene="alkA"
                     /locus_tag="ACHOSM_RS10330"
                     /old_locus_tag="pgaptmp_002066"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HGU4558584.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDMARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGCW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            complement(4333..7649)
                     /gene="dgcE"
                     /locus_tag="ACHOSM_RS10335"
                     /old_locus_tag="pgaptmp_002067"
                     /pseudo
     CDS             complement(4333..7649)
                     /gene="dgcE"
                     /locus_tag="ACHOSM_RS10335"
                     /old_locus_tag="pgaptmp_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            7967..8608
                     /gene="udk"
                     /locus_tag="ACHOSM_RS10340"
                     /old_locus_tag="pgaptmp_002068"
     CDS             7967..8608
                     /gene="udk"
                     /locus_tag="ACHOSM_RS10340"
                     /old_locus_tag="pgaptmp_002068"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGU4558585.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            8700..9281
                     /gene="dcd"
                     /locus_tag="ACHOSM_RS10345"
                     /old_locus_tag="pgaptmp_002069"
     CDS             8700..9281
                     /gene="dcd"
                     /locus_tag="ACHOSM_RS10345"
                     /old_locus_tag="pgaptmp_002069"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HGU4558586.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            9303..11156
                     /gene="asmA"
                     /locus_tag="ACHOSM_RS10350"
                     /old_locus_tag="pgaptmp_002070"
     CDS             9303..11156
                     /gene="asmA"
                     /locus_tag="ACHOSM_RS10350"
                     /old_locus_tag="pgaptmp_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HGU4558587.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIHGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAIERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKGTPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11607..13190)
                     /gene="yegH"
                     /locus_tag="ACHOSM_RS10355"
                     /old_locus_tag="pgaptmp_002071"
     CDS             complement(11607..13190)
                     /gene="yegH"
                     /locus_tag="ACHOSM_RS10355"
                     /old_locus_tag="pgaptmp_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU4558588.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(13391..13540)
                     /locus_tag="ACHOSM_RS10360"
                     /old_locus_tag="pgaptmp_002072"
     CDS             complement(13391..13540)
                     /locus_tag="ACHOSM_RS10360"
                     /old_locus_tag="pgaptmp_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4558589.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            13849..14988
                     /gene="wza"
                     /locus_tag="ACHOSM_RS10365"
                     /old_locus_tag="pgaptmp_002073"
     CDS             13849..14988
                     /gene="wza"
                     /locus_tag="ACHOSM_RS10365"
                     /old_locus_tag="pgaptmp_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HGU4558590.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            14994..15437
                     /gene="wzb"
                     /locus_tag="ACHOSM_RS10370"
                     /old_locus_tag="pgaptmp_002074"
     CDS             14994..15437
                     /gene="wzb"
                     /locus_tag="ACHOSM_RS10370"
                     /old_locus_tag="pgaptmp_002074"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HGU4558591.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            15440..17602
                     /gene="wzc"
                     /locus_tag="ACHOSM_RS10375"
                     /old_locus_tag="pgaptmp_002075"
     CDS             15440..17602
                     /gene="wzc"
                     /locus_tag="ACHOSM_RS10375"
                     /old_locus_tag="pgaptmp_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HGU4558592.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            17780..18619
                     /gene="wcaA"
                     /locus_tag="ACHOSM_RS10380"
                     /old_locus_tag="pgaptmp_002076"
     CDS             17780..18619
                     /gene="wcaA"
                     /locus_tag="ACHOSM_RS10380"
                     /old_locus_tag="pgaptmp_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HGU4558593.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            18622..19110
                     /gene="wcaB"
                     /locus_tag="ACHOSM_RS10385"
                     /old_locus_tag="pgaptmp_002077"
     CDS             18622..19110
                     /gene="wcaB"
                     /locus_tag="ACHOSM_RS10385"
                     /old_locus_tag="pgaptmp_002077"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HGU4558594.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            19107..20324
                     /gene="wcaC"
                     /locus_tag="ACHOSM_RS10390"
                     /old_locus_tag="pgaptmp_002078"
     CDS             19107..20324
                     /gene="wcaC"
                     /locus_tag="ACHOSM_RS10390"
                     /old_locus_tag="pgaptmp_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HGU4558595.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLDNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATETILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAGFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            20299..21516
                     /gene="wcaD"
                     /locus_tag="ACHOSM_RS10395"
                     /old_locus_tag="pgaptmp_002079"
     CDS             20299..21516
                     /gene="wcaD"
                     /locus_tag="ACHOSM_RS10395"
                     /old_locus_tag="pgaptmp_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HGU4558596.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIAAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGVILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLALWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            21527..22273
                     /gene="wcaE"
                     /locus_tag="ACHOSM_RS10400"
                     /old_locus_tag="pgaptmp_002080"
     CDS             21527..22273
                     /gene="wcaE"
                     /locus_tag="ACHOSM_RS10400"
                     /old_locus_tag="pgaptmp_002080"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HGU4558597.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            22289..22837
                     /gene="wcaF"
                     /locus_tag="ACHOSM_RS10405"
                     /old_locus_tag="pgaptmp_002081"
     CDS             22289..22837
                     /gene="wcaF"
                     /locus_tag="ACHOSM_RS10405"
                     /old_locus_tag="pgaptmp_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HGU4558598.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            22863..23984
                     /gene="gmd"
                     /locus_tag="ACHOSM_RS10410"
                     /old_locus_tag="pgaptmp_002082"
     CDS             22863..23984
                     /gene="gmd"
                     /locus_tag="ACHOSM_RS10410"
                     /old_locus_tag="pgaptmp_002082"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGU4558599.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            23987..24952
                     /gene="fcl"
                     /locus_tag="ACHOSM_RS10415"
                     /old_locus_tag="pgaptmp_002083"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             23987..24952
                     /gene="fcl"
                     /locus_tag="ACHOSM_RS10415"
                     /old_locus_tag="pgaptmp_002083"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HGU4558600.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            24955..25434
                     /gene="gmm"
                     /locus_tag="ACHOSM_RS10420"
                     /old_locus_tag="pgaptmp_002084"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             24955..25434
                     /gene="gmm"
                     /locus_tag="ACHOSM_RS10420"
                     /old_locus_tag="pgaptmp_002084"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HGU4558601.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFTVAEEELLLPDEQHDDYRWLTSDALLASDNVHANSRAYFLAEKRTGVPGL"
     gene            25431..26654
                     /gene="wcaI"
                     /locus_tag="ACHOSM_RS10425"
                     /old_locus_tag="pgaptmp_002085"
     CDS             25431..26654
                     /gene="wcaI"
                     /locus_tag="ACHOSM_RS10425"
                     /old_locus_tag="pgaptmp_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HGU4558602.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCAPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKAA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVAEADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            26657..28093
                     /gene="cpsB"
                     /locus_tag="ACHOSM_RS10430"
                     /old_locus_tag="pgaptmp_002086"
     CDS             26657..28093
                     /gene="cpsB"
                     /locus_tag="ACHOSM_RS10430"
                     /old_locus_tag="pgaptmp_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HGU4558603.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSSVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            28286..29656
                     /gene="cpsG"
                     /locus_tag="ACHOSM_RS10435"
                     /old_locus_tag="pgaptmp_002087"
                     /gene_synonym="manB"
     CDS             28286..29656
                     /gene="cpsG"
                     /locus_tag="ACHOSM_RS10435"
                     /old_locus_tag="pgaptmp_002087"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HGU4558604.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLLDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LALLNE"
     gene            29711..31105
                     /gene="wcaJ"
                     /locus_tag="ACHOSM_RS10440"
                     /old_locus_tag="pgaptmp_002088"
     CDS             29711..31105
                     /gene="wcaJ"
                     /locus_tag="ACHOSM_RS10440"
                     /old_locus_tag="pgaptmp_002088"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HGU4558605.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKVSSPGPVIFRQTRYGMDGKPMKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            31107..32585
                     /gene="wzxC"
                     /locus_tag="ACHOSM_RS10445"
                     /old_locus_tag="pgaptmp_002089"
     CDS             31107..32585
                     /gene="wzxC"
                     /locus_tag="ACHOSM_RS10445"
                     /old_locus_tag="pgaptmp_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HGU4558606.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIITLADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTV
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            32657..33937
                     /gene="wcaK"
                     /locus_tag="ACHOSM_RS10450"
                     /old_locus_tag="pgaptmp_002090"
     CDS             32657..33937
                     /gene="wcaK"
                     /locus_tag="ACHOSM_RS10450"
                     /old_locus_tag="pgaptmp_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HGU4558607.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVNDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IELSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGRLQAMVADTLGQ
                     LPALNTRLNDAVSRERQTGMQMVKSVLERIGEAK"
     gene            33934..35154
                     /gene="wcaL"
                     /locus_tag="ACHOSM_RS10455"
                     /old_locus_tag="pgaptmp_002091"
     CDS             33934..35154
                     /gene="wcaL"
                     /locus_tag="ACHOSM_RS10455"
                     /old_locus_tag="pgaptmp_002091"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HGU4558608.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGTESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVIKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            35165..36559
                     /gene="wcaM"
                     /locus_tag="ACHOSM_RS10460"
                     /old_locus_tag="pgaptmp_002092"
     CDS             35165..36559
                     /gene="wcaM"
                     /locus_tag="ACHOSM_RS10460"
                     /old_locus_tag="pgaptmp_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HGU4558609.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNAHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEKGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHIIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNTVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            36734..37627
                     /gene="galF"
                     /locus_tag="ACHOSM_RS10465"
                     /old_locus_tag="pgaptmp_002093"
     CDS             36734..37627
                     /gene="galF"
                     /locus_tag="ACHOSM_RS10465"
                     /old_locus_tag="pgaptmp_002093"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HGU4558610.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            38000..39085
                     /gene="rfbB"
                     /locus_tag="ACHOSM_RS10470"
                     /old_locus_tag="pgaptmp_002094"
     CDS             38000..39085
                     /gene="rfbB"
                     /locus_tag="ACHOSM_RS10470"
                     /old_locus_tag="pgaptmp_002094"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGU4558611.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDATAMARIFALHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVENVKSGAYQSWIAQNYEGRQ"
     gene            39085..39984
                     /gene="rfbD"
                     /locus_tag="ACHOSM_RS10475"
                     /old_locus_tag="pgaptmp_002095"
     CDS             39085..39984
                     /gene="rfbD"
                     /locus_tag="ACHOSM_RS10475"
                     /old_locus_tag="pgaptmp_002095"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HGU4558612.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPD
                     GVAETIRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFSGNGDMPWLETDTTAPLNVYGETKLAGEKALQEYCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPDVAGLYHLVAS
                     GTTTWYDYAALVFEEARKAGIPLSLNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            40042..>40840
                     /gene="rfbA"
                     /locus_tag="ACHOSM_RS10480"
                     /old_locus_tag="pgaptmp_002096"
     CDS             40042..>40840
                     /gene="rfbA"
                     /locus_tag="ACHOSM_RS10480"
                     /old_locus_tag="pgaptmp_002096"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGU4558613.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQASPDGLAQAFIIGEEFIGG
                     DDCALILGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIGE"
BASE COUNT        10094 a        10196 c        10913 g         9637 t
ORIGIN      
        1 aatatgaaag tatttacatc gacaggtgaa ttgaccactc ttggccgtga actgggcaaa
       61 ggcggtgaag gcgcggttta tgatatcaat gagtttgtcg atagcgtcgc caagatttat
      121 cacacgccgc cacccgcctt aaaacaggac aaacttgcct ttatggctgc gacagctgac
      181 gcgcagttgt tgaattatgt cgcctggccg caggcaacgc ttcacggtgg acgaggcgga
      241 aaagttatcg gttttatgat gccaaaagtt tctggtaaag aaccgattca tatgatctat
      301 agcccggcac atcgtcgcca gagttaccct cattgtgcgt gggattttct actctatgtt
      361 gcgcgcaata ttgcttcatc ttttgctacg gttcacgagc acgggcacgt cgtgggtgac
      421 gtaaaccaga acagttttat ggtaggtcgc gacagcaaag tggtactgat tgatagcgac
      481 tcctttcaga ttaacgccaa tggcacactg catttatgcg aagtcggcgt gtcgcatttt
      541 acgccgccag agctacaaac cttgtcgtca tttgtcggct ttgaacgcac agaaaatcac
      601 gataattttg gccttgcgtt gctgattttt cacgtcttgt ttggtggccg gcatccttat
      661 tctggtgtgc cgcttatctc tgatacgggt aatgcgctgg agacggatat tacccatttc
      721 cgttatgcct acgcgtcaga taatcagcga cgtggtttaa aaccgccgcc acgatcgatt
      781 ccgctatcga tgttaccgag tgatgttgaa gccatgtttc agcaggcatt cacggaaagt
      841 ggcgtggcaa ccgggcgtcc gacggctaaa gcgtgggtag cggcacttga ttctctgcgc
      901 caacagttaa agaaatgtcc cgtttcggca atgcatgttt atcccgctca tttgaccgac
      961 tgcccgtggt gtacgctgga taatcaaggc gttatctatt ttattgatct cggcgaagag
     1021 gtcattacca ccggcgggga ttttgtgctg gcaaaagtct gggcgatggt gatggcgtca
     1081 gtagcaccgc cagcattgca actgccatta cccgatcatt tccaaccgac tggcaggcca
     1141 cttcctttag gcctgttacg gcgcgaatac atcattctgc ttgagatcgc actgtcagca
     1201 ttatcgctgc tgctttgcgg ccttcaggca gaaccgcgtt atattatttt ggttcctgtg
     1261 ctggcggcta tctggattat tggcagtctg acaagcaaag cttataaagc agaaatccag
     1321 caacgccgtg aggcatttaa tcgcgcgaaa atggactatg accatttagt cagccagagc
     1381 caacagttgg gcgggctgga aggttttatc gccaaacggg cgatgctcga aaaaatgaag
     1441 gacgaaattc tcgggttacc ggaagaagag aaacgcgctc tggcagcact tcatgacact
     1501 gcaagggaac ggcagaagca gaagtttctg gagggatttt ttattgatgt tgcctctatt
     1561 cccggcgttg gccctgcgcg taaagcggcg ttacggtctt ttggtattga aacagccgca
     1621 gatgttaccc gtcgtggcgt taagcaagtg aaagggtttg gcgaccatct gacccaggcg
     1681 gttatcgact ggaaagcgag ctgtgaacgc cgttttgtgt tcaggccgaa tgaagcgata
     1741 acgccagcag acagacaagc ggtaatggcg aaaatgaccg ccaaacgaca tcggctggaa
     1801 tcggcgttga cagtcggcgc gacagagttg cagcgattcc gccttcatgc tccagcacgg
     1861 accatgccgt tgatggaacc gttacgtcag gcagcagaaa aactggctca ggcgcaggca
     1921 gatttaagcc gctgctgagc catttttcaa ttaacgaaac accacttccg cccagcgtgc
     1981 cagcccggcg gtgacggagc caaagtcatc gccgcctgca atcggaatgc ccggcaactg
     2041 ttctgccagc gcttttttaa tcagcggaga acgggcgcta ccgccggtca ggtagataac
     2101 gtccggtttt tcctgggcgt tatccagggc cagttgcacc tgttccagaa tccgcgccag
     2161 tggctggctg agggcgcttt ccagccctcg ctggctaatc agcgtagcca gttcattgct
     2221 gataaacggc agtgaggcgc gggtttccgc tacgcttgaa agagcaattt tgcactcttc
     2281 tgcgctacgc accaggcgat agcttaaacg ctgacgccag actttctgta acagggccac
     2341 tttttccggt tcgcgggcat cgcgtaccag atcgttaagc agacgaccgt tggcactact
     2401 gtagaaatca ctctgcgcag gtacgtcgtt gatggcaacc gcattccacc acggcaggat
     2461 cggcagggcg atgccttttt cggtttcgcc acccataccc agcagtggca tcaggttttt
     2521 aaacgccagg gcgatatcca gatcgttacc gccaatacga caaccactgt gacccagcag
     2581 gctggcttca cgatcgagac gcgaacgcca ctgcggtccc atcagcagca atgaacagtc
     2641 agtcgtacca ccgccgatat ccaccaccag cacccgtttt tcttcctgca aggtggcttc
     2701 gtaatccagc ccagccgcga ccggctcgta ctggaatacc acgtccctga atccggcacg
     2761 cttcgccgcg cgttccagaa tcccttgcgc ctgggcgttt gcttcatcgc cgcccagccc
     2821 ctggaagttg atcgggcgac caatcaccgc ctgagtaatc gcttctggca gctgcacctg
     2881 cgcctgctgg cgaatgtgca acatcattgc gcagaccaga tcctcaaaca gcgctacctg
     2941 ctgcggtttt aagccgctgg caccgaggaa cgattttggt gatttcacaa accacacttc
     3001 ttctggatca tcaatgtact gtgccagtga ggaaagaccg aactgcacgc ttttcgccgt
     3061 aacatcgata tcttcttcgc ggttataacg aatcgcccga cgtaacagcg cctgcgtttc
     3121 atcgtcgtct gccggaacat catgatggcg gtacagccat tcacttactg cttcacgcgt
     3181 tggcgcgcaa agcattgaag gcagcagcgt gctgtcgttt tccattttca gtaattgcgg
     3241 tttaccgtca cgcatgaccg ccactgaaca gtttgctgta ccgtaatcaa aaccaataaa
     3301 cacgaattaa tccccatgcc ggtgaagaag gggcgtgact ttagcgaaat gttgccgtcg
     3361 cgacaaccgg aatatgaaag caaagcgtag cgtctgaata acgtttatgc tgaaagcgga
     3421 tgaataagga gatgcgatgt ataccctgaa ctggcagccg ccgtatgact ggtcgtggat
     3481 gttgggattt ctcgccgccc gtgcagtgag tggtgtggaa acggtcgctg acgattatta
     3541 tgcccgtagt ctggcggtgg gcgaatatcg cggcgtggtg actgctattc cggatatggc
     3601 ccgtcatact ctgcacataa atttaagtgc aggtttagaa cctgttgccg ctgagtgtct
     3661 ggcgaaaatg agccgcctgc ttgatctgca atgtaacccg cagattgtta acggtgcgtt
     3721 gggcaagtta ggcgcggcgc ggcccggatt gcgtttaccc ggctgtgttg atgcttttga
     3781 gcagggtgtg cgggcgattt taggccaact ggtgagcgtg gcgatggcgg caaaattgac
     3841 tgccaaagtg gtacagcttt atggcgaacg gctggatgat tttccggagt atatctgctt
     3901 cccgacgcct cagcggctgg cagcagccga cccgcaggca ttaaaagcgt taggtatgcc
     3961 gttgaaacgg gcagaggcgc tgattcatct ggcaaatgcg gcgctggagg gcaccttacc
     4021 aatgacaata ccgggcgatg tggagcaggc gatgaaaacg ctgcaaactt ttccgggtat
     4081 cgggtgctgg acggcgaatt atttcgcttt gcgtggctgg caggcgaaag atgtttttct
     4141 gccggatgat tatctgatta aacagcgatt tccggggatg acaccggcac aaatccgccg
     4201 ttatgccgag cgctggaagc cctggcgttc ttatgcgctg ttgcatatct ggtatacgga
     4261 aggctggcaa ccagacggaa cggacgaatt gtaggcctga tacgtcgcat caggcaatgg
     4321 gcaaacgctc cctcagttaa tcgcgaaata actgctattc accagcaaat ccagcggttg
     4381 ggcatcggca atcacatcac cataaatcag atcgacgccg atgccagaaa gcgtatccat
     4441 cactaagggt aaaacgactg gcccggcgat ggttttcatc ccgagtcgct gagcgtgccc
     4501 ctgaataatc gtaatcagca tctcatccat caaattaccc tgcacgttgg cgcataactc
     4561 accatcaagt agcaggtaat ctgccatatt cgctttcagc gagttgaaga tttgcagatc
     4621 gcggcccacc tggctgagca ctatccgaca tcccgccagc cgcagttttt gcacgctttc
     4681 tgcgtgatct aaaatcgcgt ctgccggaat aatcagatgt aataaccgtg gtggtagagg
     4741 gctattttcc agctgctcaa gcagatcatt caccagcgtg gcgctactca aaccggcaac
     4801 ggaaaggggg agggcgatgc ttatgccttt actggcaacc gcttttgcgg ccagctggaa
     4861 aaattcgtgg aatacccgtc ggtcaagagc atggctaagt gccggatcgc tgaagctacg
     4921 acgaaatttt tgttcatcaa taatctcgcc ttcgcaactc cagagtttaa gcgaaatcag
     4981 ccacaaatta cgcgcttccg ggatccgcgg cgaagcgaca ccgtgggcca tcatcatcaa
     5041 ctgattctct ttaatcatcc gccactgttc atcaagcgac atcgccgccc gttcgctatg
     5101 tgcggcagct tgctgtggtt cgtaaaccgt cacccggccc cgtccaccat ttttggaggc
     5161 ataacaggcg atatcagcct gcgacatcac ttctgccgcc tgatgattgt tgtcatcaat
     5221 caaggtaatc ccggcactgg cacctacccg atgcacacgg ccttcccata taaagtgata
     5281 gtcattcacg gcactgataa tgcgtgtagc gataaaacgc gcgctttcaa cattacagtc
     5341 tggtagcagc agaccaaatt catccccacc gagtcgcgcc agcacgtcgc tggaacgcag
     5401 catactcagc atcaacgacg ccagttcgcg cagcaaagcg tcgcccgccg catgcccggc
     5461 gctgtcattc accgctttaa agcgatcaag atcgataaac accagggcat gtcgctgatg
     5521 tgtactgttt accgtttgca gcagcatacg cagttgcttc tcaaaactgg cgcgattggc
     5581 gagatgcgtc agtgcatcat gggaggcgct gtagctcagc tggcgcagca ttttgcgtga
     5641 ttcggtaacg tcctgaatca ccagaaccga accaatattg ctgccgtcca gagtacttaa
     5701 cggcgtaata ctgtaatgca cgtcgtagct gccgccgctc cggcagtgca acaccacatc
     5761 ttgttcaatc gcggaacgtg aggtgtcggc actgtaaatg ttctccatca ggggaccatt
     5821 gtcgccaaag gtaatatgca acaccgtcag gagcggaaca cctaatgctt cttcctgcgt
     5881 ccagccgctc atcttctccg ccactggatt cataaaggta attttcattg ccatatcaat
     5941 acagaccacg gcttcgccga tggagtccag cgttatgtgc aggcgctctt tttcctgaaa
     6001 caatgcctcg ttaagctgtt taacctcagt catatccata ttaatgccga gcaggcgttc
     6061 gacttcgcct tctttattca gtacccggtt ggcgagggcg cggatatggc gaataccgtc
     6121 ttttacggta atgcgaaatt ccagtttaaa gggcgagcgt gattgcaacg aatcacgaat
     6181 cactttttcg gcatgctggc gatcttccgg cagcacgcac tcgtaccaca cctgccagtt
     6241 cggtttgata tgtggaggaa tttcatacag ctcgaacatc cgcttatccc agctaaaaat
     6301 attcggcttc aactcccact cccagatgcc aatcccgccc gcttcgttag ccagcgtgat
     6361 gcgttccatc agttgctgat tcacctgttc ggtgcgtttt agctcgttaa tgtcttcaat
     6421 ctgagcgata aaatagagcg gcgtgccatc cgtgtggcgc accagtgaga cggcaagcaa
     6481 cgcccagaca acatcgccat tacggttgta gtagcgtttt tccattgaat aggtgtttat
     6541 ttcaccgctt atcagctttt caacctgttg gagatcttta ttgagatcct ccggccaggt
     6601 cagttgctga aaggtgagtc cgcgcagctc ttcctgactg tacccgagaa actggcagag
     6661 cgctttgttg gtttgcagcc attgtccctc ggtgcccact aacgccatac cgatagcgga
     6721 atattccatc gcgttccgaa agcgggtttc gctttcggaa atgtgtttgc gttccgcacg
     6781 aaaggcatac atcaccatgg tcatgatgtt ggcgggcagc aggatcagca aaaacggtag
     6841 ccacggcata tggctcatca ggtacgtacg cggcgtagca agcagggagg gatccgcggc
     6901 catcatcagc gacaccatca tcaccgtggt aaggaagatc aaaaaggcct ccattcgtgg
     6961 cagacgcacg gcgctccaca tcaacagcac aataatgaaa gtaaaaggcc acggcagata
     7021 cagcatcgaa agccagctta acgtcagtgt gatggctaac gtgagcagcg actcaaaaag
     7081 caaccgtggg ttgcgatggc gcagcagata gtgtggttta aataacaatc ccagcggcac
     7141 cagtgccaga gcgccgatgg attctgacag tacccatatc aaaaatgccc tgagaggatc
     7201 gtctccggcc gtcagcagga caaccagaac ccccccaaca gaggcggaac aatggcgctg
     7261 ccgagtgcca gacgcagcca gtcagccaga ttttgcaagg ggttatacca cggcagcaat
     7321 ttacgtagca gcactgcccc gaccacggct tcaacaatat tgatggtcgt ccaggtcatg
     7381 ttcagcgagc tggtggaaaa aagcaggatg gatgcggcga tatttcccag cgaacagctc
     7441 agcgcaattc ccggccacat gcgcccggca tggcgataaa acgccaccat catgatggac
     7501 gtcgggaacc acagtggggc gagttgggtg ccaaactgcg aaagctcaag cgagaaaagg
     7561 gtaaagataa acgagactaa acctaaactg accaggtgaa gcagcgggtg gggcagggca
     7621 attaatacat gctgtgattg tttgctcatt accgctttat ccgcaagacc aggtcgccag
     7681 tattcgtttc tgtgtttatg ctagtacaaa agttttacat tttatatgcg aattgctcat
     7741 aaagtgacgt aaaggcggat aatttgcgca actgcgtata acatttttta ccttacataa
     7801 aactgatcaa cgtaatttgc caccgggaat tctttgtcaa caattaactc tgagcgcgcg
     7861 gcaacgctat tcgactggta tcagacggat gaaatcccta taattgccgc gtttggcgct
     7921 tcgtcgcccc cttcctaaca tccaggttaa tcaggtcgct aaatatatga ctgatcagtc
     7981 tcatcagtgc gtcattatcg gtatcgctgg cgcatcggct tccggcaaga gtcttattgc
     8041 cagtaccctt tatcgtgaat tacgtgagca agtcggtgat gaacacatcg gcgtaattcc
     8101 cgaagactgc tattacaaag atcaaagcca tctgtcgatg gaagaacgcg ttaagaccaa
     8161 ctacgaccat cccagcgcga tggatcacag cctgttgctt gagcatttac aagcgttgaa
     8221 acgcggctcg gcaattgacc tgccggttta cagctatgtt gaacatacgc gtatgaaaga
     8281 aacggtgacg gttgagccga agaaggtcat cattctcgaa ggcattttgt tgctgacgga
     8341 tgcgcgtttg cgtgacgaac ttaacttctc cattttcgtt gataccccgc tggatatctg
     8401 cctgatgcgc cgcatcaagc gtgacgttaa cgagcgtggg cgttcaatgg attccgtgat
     8461 ggcgcaatat caaaaaaccg tgcgcccgat gttcctgcaa ttcattgagc cttctaaaca
     8521 atatgcggac attatcgtgc cgcgcggcgg gaaaaaccgc atcgcgatcg atatattgaa
     8581 agcgaaaata agtcagttct ttgaataagc ttgataaatt gtgtaccgtt cagtgataac
     8641 ctggtatgcc cttgacgtaa ttggcgttaa gggagtgatg cgcgaaagga gaaaatgcca
     8701 tgcgtctgtg tgaccgagat attgaagcct ggcttgatga aggccgtttg tcgatcaacc
     8761 cacgtccgcc agtggagcgt attaacggcg cgacggtgga tgtacgcctg ggcaataaat
     8821 ttcgtacctt ccgtggtcac acggcagcgt ttatcgatct gagcggtccc aaagatgaag
     8881 tgagcgccgc gcttgaccgc gtgatgagcg atgagatcgt tctcgacgag ggcgaggcgt
     8941 tctatcttca cccaggagag ctggcgctgg cggtgacgct ggagtcggtg acgctgccag
     9001 ccgatctggt gggctggctg gacgggcgtt cctctttggc gcgtctgggg ttgatggtgc
     9061 acgtcaccgc gcaccgtatc gatccgggct ggtctggttg cattgtgctg gagttctaca
     9121 actccggtaa gctgccgctg gcgctgcgtc cgggcatgtt aattggtgcg ctgagctttg
     9181 agccgctttc cggcccggcg gcgcgacctt acaaccgccg tgaagatgcg aaatatcgca
     9241 accagcaggg cgcggtagcc agccgaatcg ataaagacta attcaggccc attgaggata
     9301 ccatgagacg atttctgacg acgctgatga tactcctggt cgtgctggtg gccgggttat
     9361 ctgcgttagt gttgctggtg aatccgaatg atttccgcga ctatatggtc aagcaagttg
     9421 ctgcacgtag cggttatcaa ttgcagctcg acgggccact gcgttggcac gtctggccgc
     9481 agcttagtat cctctccggg cgaatgtctc tcaccgccca gggcgcaagc cagccactgg
     9541 ttcgcgccga caacatgcgt ctggacgtgg cgcttttacc actactgagt catcaactga
     9601 gcgttaagca ggtgatgcta aaaggggcag tgatccaact gacgccgcag acggaagcgg
     9661 tgcgcagtga agacgctccg gttgcaccgc gcgacaatac cttgccggat ctgtcagacg
     9721 atcgcggatg gtcgtttgat atatccagtc ttaaggtggc ggacagcgtg ctggtgttcc
     9781 agcatgaaga tgacgagcag gtgacaatcc gcaatattcg cctgcaaatg gaacaagatc
     9841 cccaacatcg tggttcattt gagttctccg ggcgggttaa tcgcgatcag cgcgatctca
     9901 cgatatccct taacggtacg gtagatgctt ctgattatcc gcatgattta acggcggcta
     9961 ttgaacaaat taactggcag ttgcagggtg cagatttacc aaaacaaggt attcatgggc
    10021 aggggagttt ccaggcccag tggcaggagt cacataaacg cctttcattt aaccaaatca
    10081 gcttgaccgc caatgacagc acgctgagcg ggcaagcaca ggtcacgctg acagagaaac
    10141 ctgaatggca gctgagactg caattcccgc aactgaatct tgacaacctc atcccgctta
    10201 atgaaaccgc gaatggtgaa aacggtgccg cgcagcaggg gcagagccaa tcaacgttgc
    10261 cgcgtccggt catttcttcg cgtattgatg aaccggccta tcagggactg caaggcttta
    10321 cggctgatat tttgttgcag gccagtaacg tgcgctggcg cggaatgaat tttacagatg
    10381 ttgccacgca aatgaccaac aagtcgggtt tgctggaaat tactcaactg cagggcaaac
    10441 ttaacggtgg acaggtttca ctgccgggca cgctggacgc gacatcaata aatccgcgga
    10501 taaacttcca gccacggctg gaaaacgttg agattggtac cattctgaag gcgtttaact
    10561 atccgatttc gttgaccgga aaaatgtcac tggctggtga tttctccggt gctgacatag
    10621 atgccgacgc attccgccac aactggcaag gacaggcaca tgtcgaaatg accgacacgc
    10681 gcatggaagg gatgaacttc cagcagatga ttcagcaagc gatagagcgt aatggcggtg
    10741 atgtgaaggc cgctgaaaac tttgataacg tgacgcggct tgaccgcttt accaccgatt
    10801 tgacgttgaa ggatggcgtc gtgacgttaa acgacatgca aggtcaatcg cctgtgctgg
    10861 cgctgacagg ggaaggcatg ttgaatctgg cagatcaaac ctgcgatacc cagtttgata
    10921 ttcgggtcgt gggtggctgg aacggggaaa gtaaactgat tgatttcctg aaaggaacgc
    10981 cagtaccgct gcgggtttat ggcaactggc agcaactcaa ttacagtctg caagtggatc
    11041 agttactgcg caaacatcta caggacgaag cgaaacgtcg cctgaatgac tgggccgagc
    11101 ggaataaaga ttcccgcaat ggcaaagatg tgaagaagtt gctggagaag atgtagatta
    11161 ccaggtgacc agtcctgtag gcctgataag acgcgtaaag cgtcgcatca ggcgttcgtg
    11221 gcggcatcat tatattctga atatgtaaga tcgaaggctg ttccgttcac acttgatgtt
    11281 cctcgttact atggaacgct caagccggtg aacgtgccag ggtggctcaa tctccaccac
    11341 cctggcaacc cgggctcccg gcggtaaatc gccgctggca cggttccctc atcttattcc
    11401 tttcggctat cgggtacggg cgcgaggtaa catatatgga cgcgccctca gcccacatcc
    11461 atgtgggctg ccccggccta caggaaacga ttcggcgatt tacagccgga cccacacccc
    11521 cgctgtgatg tccgtttacc acagacaacg cttcattgct gaagttataa ggcgttacgc
    11581 agcattgggt aaaatgaccg ataccatcac acctcgtact ccaactcgcc atccttatgc
    11641 agcggaataa tctgcacctt ctgcacgcga tggctttcta cctgcaacgt tttaagcaaa
    11701 taatccccca cctgaacttc ttcgccaggt tttggaatac gctgtaaata ttccatcaac
    11761 agcccggcaa tggtgtgata ttcacgtttt tcatccagcg gcagcggtac atattgcacc
    11821 agatcttcca gcggcatatg accattcgcc gtccaggaac cgtccgcatt cttctgaata
    11881 tcatggcggg cgtcgatctc ttccacttcg ttcggtaagt taccggcaat ggtttcagtg
    11941 acgtcactta atgtcacaat cccttccacc gagccaaact catccaccac aaaagcaaag
    12001 tgcgtgcggg cattacggaa ctgctccagg gcaggtaaca acggcaaggt ttccgggaac
    12061 accagcggct ggcgaatcaa cacccgcagg ttgagcggtt cgccgcgcag agactgttgt
    12121 agcaggtcga taacgtgaac aacaccgagc aaatcttctg cgtcatcacc gtcggtaacc
    12181 accagccgcg tatgctgatt tcgctccagc aactggcgga tttcttcttc cggcgcgttg
    12241 agatcgatat gctcaatatc gtggcgcgac gtcataatgc tgctgacggt acgctggtta
    12301 agattaagta cccgctcaat catccgccgt tcctgcggat taaagatctg ctggtttcca
    12361 tgatccacca gcatggacgc ggtttcggcg tccagttccg catcttcttt ttgcccgcta
    12421 agcaggcgca ttaccgcttc ggtagtccgc tggcgcagcg tctggttagc ggaaagaaaa
    12481 cgtcgccggt taaagatagc caactgattg agcgcctcga tcatcaccga gaagccaatg
    12541 gcggcgtaca ggtagccttt cggaatgacg aagccgaaac cttctgccac caggctaaag
    12601 ccaatcatca acaggaagct taagcagaga ataacgatgg tcgggtgact gttaacaaat
    12661 tgcgttaacg gcttgctggc catcaacatc aggctgatag cgataaccac ggcggccatc
    12721 atgaccagta aatgatcgac catcccgacg gcggtaatca ctgagtcgag tgagaagatg
    12781 gcgtccagta ccacaatttg tgtcaccacc ccccagaact tcgcaccttt gcgttgtgtg
    12841 ggattattgc tgtctttccc ttccagccgt tcgttcagct ccatcgtggc tttgaacaac
    12901 aggaagaaac caccaaacag catgattaag tcgcgggcgc taaaggtaaa cgagcggaag
    12961 ctgaacagcg gttgagtcag ggtgaccagc caggagattg acgccagcag taacaggcgc
    13021 attaacatcg ccagcagcag cccggtaacc cgtgcgcggt cgcgctgctt cggcggtagt
    13081 ttttcggcga ggatggcaat aaagaccagg ttatcaatgc cgaggaccag ttcgatcaca
    13141 atcagcgtga ttaacccggc ccagatagac ggatcggcaa tccattccat agtaagtaca
    13201 aaacctattc gttgtatgac aaatgtcaca attcgatcat ggataaatcg gggcaaaaat
    13261 gcaatgttgc agcactattt atctcatatt atttgagggt aataacctca cattatccct
    13321 gaattaaaag tggtaataat aaaacattat ttaagaaata tcgcatgtgg agacgcaata
    13381 ttaaatattg tcaacctaaa gaaactccta aaaaccatat tgaatgacac ttaatataat
    13441 tcttaaaaat agccaattgc cgaattgtta ccttgcctgc tattccgtta gctgtaacac
    13501 ttcctcctgc attattggag agccaatatt caattaccat aatgtcacct tatattcgga
    13561 agttaatttc cgcaataacc ctttctcatc aacgactgta tgttgagagg gttttaaaaa
    13621 ttaagctaaa tcagtgtatt ggtagctaaa aagccagggg cggtagcgtg tctggatgcc
    13681 tgaaagacca gtctgaatta ttctgtcaat agcctgcgga tgaaatcaat ttttttagga
    13741 ctgatgccag ttaaattttt attcaattaa ctgcaaggta aatgtgcatg gcgacattgc
    13801 tattaatagt gcacaggata attactctgc caaagtgata aataaacaat gatgaaatcc
    13861 aaaatgaaat tgatgccatt attggtgtca gtaaccttga taagcggttg cacagtactt
    13921 ccgggcagca atatgtcgac gatgggcaaa gacgtcatca aacagcagga cgctgatttc
    13981 gatctcgaca aaatggtgaa tgtttatccg ctgactccgc gcctgattga ccaattacgc
    14041 ccacgcccga atgtagcgcg ccccaatatg acgctggaaa gtgagatcgc gaattaccag
    14101 tatcgcgtcg ggccggggga cgttcttaat gtcaccgtct gggatcaccc ggaactcacc
    14161 acgccagccg gtcagtaccg cagctccagc gacaccggca actgggtaca gcctgacggc
    14221 acaatgtttt atccgtatat cggcaaggtt cacgtagtgg ggaaaacgct cgctgaaatc
    14281 cgcagtgata ttaccgggcg cttagcaacg tacatcgctg acccgcaggt ggacgttaat
    14341 atcgccgcct tccgctcaca aaaggcctat atctcaggtc aggtgaataa atccggtcaa
    14401 caggcgatca ccaacgtacc actgaccatt ctcgacgcca tcaacgccgc aggtggcctg
    14461 accgacaccg ctgactggcg caacgtggtg ctaacacaca atggtcgtga agaacgcatt
    14521 tctttgcagg cgctgatgca aaacggcgac ctcaatcaga accgactgct ttatcccggc
    14581 gatattctct acgtgccgcg taatgatgat ctgaaagtat ttgtgatggg tgaagtgaag
    14641 aaacagagca ccctgaaaat ggactttagc ggcatgaccc tgactgaagc tctgggcaat
    14701 gccgaaggta tcgacatgac cacctccaac gccagcggca tctttgtcat tcgtccgctg
    14761 aaaggcgagg gtgggcgtaa cggcaagatt gccaatatct accagctgga tatgtccgat
    14821 gcgacgtcgc tggtgatggc gacagaattc cgcctgcaac cttatgacgt ggtgtatgtc
    14881 accaccgccc cggtttcccg ctggaaccgt ctgatcaatc agttgctgcc aactattagc
    14941 ggtgtccgtt acatgacgga tacagccagc gacattcata actggtaatc gtcatgttta
    15001 acaacatatt agttgtctgt gtcggcaata tttgccgttc cccgacggcg gaacgcttac
    15061 tgcaacgtta tcacccggaa ctgaaagtgg agtccgctgg actcggcgcg ctggtcggta
    15121 agggcgctga tcctaccgct atcagcgtcg ccgcagaaca tcaactgtct ctggaaggcc
    15181 actgtgcacg acaaatcagc cgccgcctgt gtcgcaacta tgacctgatt ttgaccatgg
    15241 aaaagcgcca tatcgaacgc ttatgcgaga tggcacctga gatgcgcggc aaagtgatgc
    15301 tgtttggtca ctgggataac gaatgtgaaa tccccgatcc gtatcgcaaa agccgggaaa
    15361 cgtttgcagc ggtgtacaca ttacttgaac ggtctgcccg ccagtgggcg caggcattga
    15421 acgcagagca ggtataagaa tgacagaaaa agtaaaacaa catgccgctc cggtaacggg
    15481 cagtgatgaa atcgatattg gtcgcctggt cggcaccgtc attgaagcgc gctggtgggt
    15541 gattggcatc accgctgtat ttgccctctg tgccgtagtt tacaccttct tcgccacgcc
    15601 gatttatagt gccgacgcac tggtacaaat cgagcaaaac agcggcaatt cgttagtgca
    15661 ggacattggt tcggcattag ccaacaaacc gcctgcatcg gacgccgaga tccagttgat
    15721 tcgttcgcgt ctggtgcttg gtaaaacggt ggacgatcta gacctcgata ttgcggtgag
    15781 caaaaacacg ttcccgattt tcggtgcggg ttgggatcgc ctgatgggac gtcagaacga
    15841 gacggtgaaa gtgaccacct ttaaccgccc gaaagagatg gcggaccagg tgtttacgct
    15901 taatgtgctg gatgacaaaa actacactct gagcagtgat ggcggcttta gcgcccgtgg
    15961 gcaagcgggc cagatgctga aaaaagaagg cgtcacgctg atggttgaag ctattcacgc
    16021 cagcccgggc agtgagttta ctgtcaccaa atactccacg ctggggatga tcaaccaact
    16081 gcaaaacagc ctgacggtaa cggagaacgg caaagatgct ggcgtactga gcctgactta
    16141 taccggtgaa gatcgcgaac agatccgcga cattcttaac agcatcgccc gtaactatca
    16201 ggaacaaaat attgagcgca aatcggcgga agcgtcgaaa agcctcgctt tcctcgcgca
    16261 acagttaccg gaagtacgta gccgcctcga tgttgccgaa aacaaactga atgccttccg
    16321 tcaggataaa gattctgttg atctgccgct ggaagcgaaa gcggtgctcg attcgatggt
    16381 gaacatcgac gcgcagttga acgaactgac ctttaaagag gcggaaatct ccaagctgta
    16441 caccaaagtt cacccggcgt accgcacgct gctggagaaa cgtcaggcgc tggaagaaga
    16501 gaaagccaaa cttaacggtc gcgtaacggc gatgccgaaa acccagcagg aaattgtccg
    16561 tctgacccgc gatgtcgagt ctggtcagca ggtctatatg caactgctga ataaagagca
    16621 ggagctgaaa atcaccgaag ccagcaccgt cggcgatgtg cgcattgttg acccggcaat
    16681 cacccagcct ggcgtgctaa aaccgaagaa agggctgatt atcctcggcg cgattatcct
    16741 tggcctgatg ctctctatcg tgggtgtgct gctgcgctcg ttgtttaatc gcggcatcga
    16801 aagcccgcag gtgctggaag aacacggtat cagcgtctat gccagcatcc cgctgtcgga
    16861 gtggcagaaa gcgcgcgata gcgtcaaaac catcaaaggg gttaaacgct ataagcagag
    16921 ccagctactg gcggtgggga atccaaccga tctggcgatt gaagccatcc gcagtctgcg
    16981 taccagtctg cacttcgcga tgatgcaggc gcagaacaat gtgttgatga tgacaggggt
    17041 tagcccgtca atcggtaaaa cctttgtctg cgccaacctg gcggcggtga tcagtcagac
    17101 caataaacgg gtgttgttga tcgactgcga tatgcgcaaa ggctacaccc acgagctgtt
    17161 gggcactaat aacgtcaatg gcctgtcgga aattctgatt ggtcagggcg atattactac
    17221 cgctgctaaa ccgacctcta ttgccaaatt tgacctgatc ccgcgcggtc aggtaccgcc
    17281 aaatccttct gaactgttga tgagcgaacg ctttgccgaa ctggtgaact gggcgagtaa
    17341 aaactatgac ctggtgttga ttgatacgcc gccgattctg gcagtgaccg atgcggcaat
    17401 tgttggtcgt catgttggaa ccacgttaat ggtggcgcgt tatgcggtca acacattgaa
    17461 agaagtggaa accagtctga gccgctttga gcaaaacggt attccggtga aaggggtgat
    17521 tctgaactcc atcttccgcc gcgccagcgc gtatcaggat tatggctatt acgaatacga
    17581 atataagtcg gatgcgaaat aacgacatcc gctgtcggat gcgatgctga cgcatcttat
    17641 ccgacctacg ctcacgccgc atccggcaag caaaccagct ccgtaggccg gataaggcgc
    17701 tcacgccgca tccggcaaca gaacccgtaa acaagcttat aaaccggcag aacatctcga
    17761 cactccggga gaacaaccta tgaaagacaa tccgctgatc tcaatctata tgccgacctg
    17821 gaaccgccaa caactggcga ttcgggcgat aaaatcggtc ttgcgccagg actacagcaa
    17881 ctgggagatg atcatcgtgg atgattgctc cacctcctgg gaacagctgc aacagtacgt
    17941 caccgccctc aacgatccgc gtatcaccta cattcacaac gacattaaca gcggggcgtg
    18001 cgcggtacgt aaccaggcga ttatgctggc gcaaggggaa tacatcaccg ggatcgatga
    18061 tgatgatgaa tggacgccca accgcctgag cgtcttcctc gcccataaac agcaactggt
    18121 cacacacgcc tttttgtacg ctaacgacta cgtttgccag ggagaagttt attcccaacc
    18181 ggcaagctta ccactgtatc ccaaatcgcc gtactcacgc cgcctgttct ataaacgcaa
    18241 tatcattggc aatcaggtct ttacctgggc atggcgtttc aaagagtgtt tgttcgatac
    18301 cgaactgaaa gcagcacagg attacgacat cttcctgcgg atggtggtgg agtacggcga
    18361 accgtggaaa gtggaagagg cgacgcagat cctgcatatc aatcacggcg aaatgcagat
    18421 cacctcgtca ccgaaaaaat tctccggata cttccacttt taccgcaagc acaaagacaa
    18481 attcgaccgc gccagtaaaa aatatcagct gtttaccctc taccagatcc gcaataaacg
    18541 tatgacctgg cgcaccttgt tgacgctgct gtcggtgcgt aacggcaaac gtctggctga
    18601 tggtattcgg gggcgctaaa gatgctggaa gatctgcgcg ccaacagctg gagtttacgc
    18661 ccgtgctgca tggtacttgc ctatcgcgtc gctcattttt gttcggtgtg gcgcaaaaag
    18721 aacgtcctca acaatctgtg ggcggccccg ctgctggtgc tgtatcgcat tatcaccgaa
    18781 tgctttttcg gttatgaaat ccaggccgcc gcgaccattg gccgccgctt tactatccat
    18841 cacggttacg ccgtagtgat caataaaaac gtggtggcgg gggatgattt caccattcgc
    18901 cacggcgtca ctatcggcaa tcggggtgcc gataacatgg catgtccaca cattggcaac
    18961 ggcgtcgaac tcggtgccaa cgttattatt cttggtgata tcacgcttgg taacaatgtc
    19021 accgtgggcg cgggcagcgt ggtgctcgac tctgtcccgg acaacgcgct ggtggtggga
    19081 gaaaaagcgc gagtgaaggt aattaaatga atattttgca atttaatgtg cgactggcgg
    19141 aaggcggggc agcaggtgtg gcgttagatc ttcaccagcg tgcgctgcaa caggggctgg
    19201 cgtcacattt tgtctacggt tacggcaaag gcggcaaaga gagcgtcagc catcagaact
    19261 atccgcaggt catcaaacat acgccgcgga tgaccgcaat ggcgaatatt gccctgtttc
    19321 gtctgtttaa tcgcgatctg tttggcaatt tcaatgagtt atatcgcacc attacccgca
    19381 caccgggtcc ggtggtcctg cattttcatg tgctgcacag ctactggcta aatcttaaga
    19441 gcgtggtgcg cttttgcgaa aaggtgaaaa accacaaacc ggatgtcact ctggtctgga
    19501 cgctgcacga tcactggagc gttaccggac gctgcgcctt taccgacggt tgtgaaggct
    19561 ggaaaacggg ctgccagaaa tgcccgacct tagataacta tccgccggtg aagattgatc
    19621 gggcgcacca gctggtggcg ggcaaacgcc agttattccg tgagatgctg gcgctgggct
    19681 gtcagtttat ttcccccagc cagcatgtgg ctgacgcttt caatagcctg tacggtccag
    19741 ggcgttgccg gattatcaat aatggcattg atatggcaac agaaacgatt ctggcggatc
    19801 tgcctccggt gcgcgaaacc cagggtaagc cgaaaatcgc ggtggtggcg catgacctgc
    19861 gttacgacgg caaaactaac cagcaactgg tgcgtgagat gatggcgctg ggcgacaaaa
    19921 tcgaactgca tacctttggt aagttctcgc cgttcaccgc tggcaacgtg gttaatcacg
    19981 gctttgaaac tgacaagcgc aagttgatga gcgcgctcaa tcagatggat gcgctggtgt
    20041 tcagttctcg cgtcgataac tacccgctga ttttgtgtga ggcgctatcg attggcgtgc
    20101 cggtgattgc cacccatagc gacgcggcgc gggaagtgtt gcaaaaatcc ggcggtaaaa
    20161 ccgtcagcga agaagaggtg ctgcaactag tgcaattaag caaaccggaa atcgcgcagg
    20221 cgatatttgg taccacgttg gccgggttca gccagcgcag ccgcgccgcc tacagtggac
    20281 aacagatgct ggaggagtat gtcaacttct atcagaatct gtagctacct gctgctgccg
    20341 ctgatctacc tgctggttaa cgtcaaaatc gcccagcttg gtgaaagttt ccccatcact
    20401 atcgtcactt ttttacctgt cttgttactg ctgtttttag aacgcatcag cgttaaaaaa
    20461 ttgatgattg ccttagggat tgctgcggga ctcacggcgt ttaactacct gtttggtcag
    20521 tcgctggatg ccagtaaata cgtcacttca accatgctgt ttgtctatat tgtgatcatt
    20581 attggcatgg tgtggagtat tcgttttaaa acaatttcgc cacacaacca tcgcaagata
    20641 ttacgtttct tttatctggt ggtcgggctg gtggtggcgc tggcggcagt ggagatggcg
    20701 caaattatcc tcaccggtgg cagcagtatt atggagtcga tttcgaaata tctgatttac
    20761 agcaacagct atgtgctgaa tttcattaaa ttcggcggca agcgcacgac agcactttat
    20821 ttcgaaccgg catttttcgc tctggcatta atctcaattt ggctcagcat caaacagttt
    20881 ggtatcaaaa cgcctaaaac cgatgctatg attctcgcag gggtaatatt atccggatcg
    20941 ttttcagggg ttatgacctt tatcctgttt tatttgctgg agtgggcatt tcaatatctg
    21001 aataaagagg cgattaagaa aaagttaccg ttggcattga tttctctggc tgtattcctg
    21061 gttggtgtgg taatcgcgtt tccttatatt tccacccgtc tgggcgattt aggtacggaa
    21121 ggatcgtcat cgtattatcg tattgtcggt ccgctggtga tggtcggtta ttctttgacc
    21181 catattgacg gtgtagtcag atttggctca ctttatgaat atgtcgcatc attcggaata
    21241 tttaacggtg cggatgtcgg aaaaaccata gacaatggtt tgtatctgct gattatttat
    21301 ttttcctggt tcgcagtgtt tttagcgctg tggtacatgg ggaaagtgat aaaaatgatg
    21361 atcaacgctt ttggtgataa ccgcaatttt cgcgtgcaat tatatctttt tactccggtg
    21421 tcgctgtttt ttaccggttc gatatttagc ccggaatatg catttttaat cgtctgtccg
    21481 tttattttgc gaaaagcgtt aaatattacg aggtaagaat aagaacatgt tgcttagcat
    21541 aatcactgtc gcgtttcgta acctcgaagg gatagtcaaa acacatgcct cgctggcgca
    21601 tctggcgcag gcggaagata tcagcttcga atggattgtt gtcgatggcg gttccaatga
    21661 cggcacccgt gagtatctgg aaaatctcaa tggtatctat aacctacgct ttgtcagcga
    21721 gccagataac ggtatctacg acgccatgaa caaaggcatt acgatggcac aaggcaagtt
    21781 cgcgttgttt ctcaattcgg gcgatatttt tcatcaggat gccgcaaatt ttgtccgtaa
    21841 gttaaaaatg caaaaagata acatgatgat caccggcgat gcgctgctgg attttggcga
    21901 cgggcataaa attaaacgta gcgccaaacc gggctggtat atttatcaca gcctgcccgc
    21961 cagtcatcag gcgatatttt tccctgtatc cggcttgaaa aaatggcgtt atgacctgga
    22021 atataaagtt tcttccgact acgcgctggc agccaaaatg tataaagccg gttatgcatt
    22081 taaaaaactc aatggcctgg tgtctgaatt ttccatgggt ggggtatcta ccaccaataa
    22141 tatggaattg tgtgctgacg cgaaaaaagt ccaacgacaa atattacatg tgcctggctt
    22201 ttgggctgaa ttatcctggc atttacgcca acgtactacc tcaaagacga aagccttata
    22261 taacaaagtc tgaatataag gaaaaccaat gcaagattta agcggtttct cggtgccgaa
    22321 agggttccgg ggcggcaacg ctattaaagt gcaattatgg tgggcagtac aggcaacaat
    22381 atttgcctgg tcgccacaag tattgtatcg ctggcgggct tttttattac gtttattcgg
    22441 agcaaaaata ggaaaaaacg tagttattcg tccgtcagta aaaattacct atccgtggaa
    22501 attaacctta ggtgattacg cgtgggtcgg cgatgacgtc aatttatata ccctcggtga
    22561 aataaccatt ggcgcacatt cggtgatatc gcaaaaaagt tatttatgca ccggtagcca
    22621 cgaccatgca agtcaacatt tcaccattaa cgccacgcct attgtgattg gcgagaaatg
    22681 ctggctggca accgatgtct ttgttgcccc aggcgtcaca atcggcgacg gcaccgtcgt
    22741 gggtgcacga agcagtgttt ttaaatcgct tccggcaaat gtggtttgcc gggggaatcc
    22801 cgcagtggtg atacgcgaac gcgttgaaac tgaataaatt caaaaataca gaggaataag
    22861 acatgtcaaa agtcgctctc atcaccggtg taaccggaca agacggttct tacctggcag
    22921 agtttctgct ggaaaaaggt tacgaggtgc atggtattaa gcgtcgtgca tcgtccttca
    22981 acaccgagcg cgtggatcac atttatcagg atccgcacac ctgcaacccg aaattccatc
    23041 tgcattatgg cgacctgagt gatacctcca acctgacgcg cattttgcgt gaagtgcagc
    23101 cggatgaagt gtacaacctg ggcgcaatga gtcacgttgc ggtttctttt gagtcaccgg
    23161 aatataccgc agacgtcgat gcgatgggta cgctgcgcct gctggaggcg atccgcttcc
    23221 tcggtctgga aaagaaaacc cgtttctatc aggcttccac ctctgaactg tacggtctgg
    23281 tgcaggaaat tccacagaaa gaaaccacgc cgttctaccc gcgatctccg tatgcggtcg
    23341 ccaaactgta tgcctactgg atcaccgtta actaccgcga atcctacggc atgtacgcct
    23401 gtaacggtat tctcttcaac catgaatccc cgcgccgcgg cgaaaccttc gttacccgca
    23461 aaatcacccg cgcaatcgcc aacatcgccc aggggctgga gtcgtgcctg tacctcggca
    23521 atatggattc cctgcgtgac tggggccacg ccaaagacta cgtaaaaatg cagtggatga
    23581 tgctgcaaca ggaacagccg gaagatttcg ttatcgcgac cggcgttcag tactccgtgc
    23641 gtcagttcgt ggaaatggca gcggcacagc tgggcatcaa actgcgcttt gaaggcacgg
    23701 gtgttgaaga gaagggcatt gtggtttccg tcaccgggca tgacgcgccg ggcgttaaac
    23761 cgggtgatgt gattatcgcc gttgatccgc gttacttccg tccggctgaa gttgaaacgc
    23821 tgctcggcga cccgaccaaa gcgcacgaaa aactgggctg gaaaccggaa atcaccctca
    23881 gagagatggt gtctgaaatg gtggctaatg acctcgaagc ggcgaaaaaa cactctctgc
    23941 tgaaatctca cggctacgac gtggcgatcg cgctggagtc ataagcatga gtaaacaacg
    24001 catttttatc gctggccatc gtgggatggt cggttctgcc atcaagcggc agctcgaaca
    24061 gcgcggtgat gtggagctgg tattacgtac ccgcgacgag ctgaacctgc tggacagccg
    24121 cgccgtgcat gatttctttg ccagcgaacg cattgaccag gtctatctgg cggcggcgaa
    24181 agtgggcggc attgttgcca acaacaccta tccggcggat ttcatctacc agaacatgat
    24241 gattgagagc aacatcattc acgccgcgca tcagaacgac gtgaacaaac tgctgtttct
    24301 cggatcgtcc tgcatctacc cgaaactggc aaaacagccg atggcagaaa gcgagttatt
    24361 gcagggcacg ctggagccga ccaacgagcc ttacgccatt gccaaaatcg ccgggatcaa
    24421 actgtgcgaa tcatacaacc gccagtatgg acgcgattac cgctctgtca tgccgaccaa
    24481 cctgtacggg ccgcatgaca acttccaccc gagtaactcg catgtgatcc cagcattgct
    24541 gcgtcgcttc cacgaggcga cggcacagaa tgcgccggac gtggtggtat ggggcagcgg
    24601 tacaccgatg cgtgaattcc tgcacgtcga tgacatggcg gcggcgagca ttcatgtcat
    24661 ggagctggcg catgaagtct ggctggagaa cacccagccg atgctgtcgc acattaacgt
    24721 tggtacgggc gttgactgca ctatccgcga gctggcgcaa accatcgcca aagtggtggg
    24781 ttacaaaggt cgggtggttt ttgatgccag caaaccggat ggtacgccgc gcaaactgct
    24841 ggatgtgacg cgcctgcatc agcttggctg gtatcacgaa atctcactgg aagcggggct
    24901 tgccagcact taccagtggt tccttgagaa tcaagaccgc tttcgggggt aatgatgttt
    24961 ttacgtcagg aagactttgc cacggtagtg cgctccaccc cgcttgtctc tctcgacttt
    25021 attgtcgaga acagtcgcgg cgagtttctg cttggcaaaa gaaccaaccg cccggcgcag
    25081 ggttactggt ttgtgccggg agggcgcgtg cagaaagacg aaacgctgga ggccgcattt
    25141 gagcggctga cgatggcgga actggggctg cgtctgccga taacagcagg ccagttttac
    25201 ggtgtctggc agcactttta tgacgataac ttctctggca cggatttcac cactcactat
    25261 gtggtgctcg gttttcgctt cacggtagcg gaagaagagc tgttactgcc ggatgagcag
    25321 catgacgatt accgctggct gacgtcggac gcgctgctcg ccagtgataa tgttcatgct
    25381 aacagccgcg cctattttct cgctgagaag cgtaccggag tacccggatt atgaaaatac
    25441 tggtctacgg cattaactac tcgccggagt taaccggcat cggcaaatac accggcgaga
    25501 tggtggaatg gctggcggca caaggtcatg aggtgcgggt cattaccgca ccgccttact
    25561 acccgcaatg gcaggtgggc gagaactatt ccgcctggcg ctacaaacga gaagaggggg
    25621 ccgccacggt gtggcgctgc ccgctgtatg tgccaaaaca gccgagcacc ctgaaacgcc
    25681 tgttgcatct gggcagtttt gccgtcagca gtttctttcc gctgatggcg caacgtcgct
    25741 ggaagccgga tcgcattatt ggcgtggtgc caacgctgtt ttgcgcgccg ggaatgcgcc
    25801 tgctggcgaa actctctggt gcgcgtaccg tgctgcatat tcaggattac gaagtggacg
    25861 ccatgctggg gctgggcctt gccggaaaag gcaaaggcag caaagcggca cagctggcaa
    25921 cggcgttcga acgtagcgga ctgcataacg tcgataacgt ttccacgatt tcgcgttcga
    25981 tgatgaataa agccatcgaa aaaggcgtgg cggcggataa cgtcatcttc ttccccaact
    26041 ggtcggaaat tgcccgtttt cagcatgttg cagaggccga tgttgatgcc cttcgtaacc
    26101 agcttggcct gccggataac aaaaaaatca ttctttactc cggcaatatt ggtgaaaagc
    26161 aggggctgga aaacgttatt gaagctgccg atctcctgcg cgatgaaccg ctgatttttg
    26221 ccattgtcgg gcagggcggc ggcaaagcgc ggctggaaaa aatggcgcag cagcgtggac
    26281 tgcgcaacat gcaatttttc ccgctgcaat cgtatgacgc tttacccgca ctgctgaaga
    26341 tgggcgattg ccatctggtg gtgcaaaaac gcggcgcggc agatgccgta ttgccgtcga
    26401 aactgaccaa tattctggcg gtaggcggta acgcggtgat taccgcagaa gcccacacag
    26461 aactggggca gctttgcgaa acctttccgg gcattgcggt ttgcgtagaa ccggaatcgg
    26521 tcgaggcgct ggtggcgggg atccgtcagg cgctcctgct gcccaaacac aacacggtgg
    26581 cacgtgaata tgccgaacgc acgctcgata aagagaacgt gttacgtcaa tttataaatg
    26641 atattcgggg ataattatgg cgcagtcgaa actctatcca gttgtgatgg caggtggctc
    26701 cggtagccgc ttatggccgc tttcccgcgt actttacccc aagcagtttt tatgcctgaa
    26761 aggcgatctc accatgctgc aaaccaccat ctgccgcctg aacggcgtgg agtgcgaaag
    26821 cccggtggtg atttgcaatg agcagcaccg ctttattgtc gcggaacagc tgcgtcaact
    26881 gaacaaactc accgagaaca ttattctcga accggcaggg cgtaacactg cacctgccat
    26941 tgcactggcg gcgctggcgg caaaacgtca tagcccggag agcgacccgt taatgctggt
    27001 gctggcggcg gatcatgtga ttgccgatga agacgcgttc cgtgccgccg tgcgtaatgc
    27061 catgccgtat gccgaagcgg gcaagctggt gaccttcggc attgtgccgg atctacctga
    27121 aaccggatat ggctatattc gtcgcggtga agtgtcggcg ggtgagcagg atacggtggc
    27181 ctttgaagtg gcgcagtttg tcgaaaaacc gaatctggaa accgcccagg cctatgtggc
    27241 aagcggcgaa tattactgga acagcggcat gttcctgttc cgcgccggac gctatctcga
    27301 agaactgaaa aaatatcgcc cggatattct cgatgcctgt gaaaaagcga tgagctccgt
    27361 cgatccggat cttgatttta ttcgtgtgga tgaagaagcg tttctcgcct gcccggaaga
    27421 gtcggtggat tacgcggtca tggaacgtac ggcagatgct gttgtggtgc cgatggatgc
    27481 gggctggagc gatgttggtt cctggtcttc attatgggag atcagcgccc acaccgccga
    27541 gggcaacgtt tgccacggcg atgtgattaa tcacaaaact gaaaacagct atgtgtatgc
    27601 cgaatctggc ctggtcacca ccgtcggggt gaaagatctg gtagtggtgc agaccaaaga
    27661 tgcggtgctg attgccgacc gtaacgcggt acaggatgtg aaaaaagtgg tcgagcagat
    27721 caaagccgat ggtcgccatg agcatcgggt gcatcgcgaa gtgtatcgtc cgtggggcaa
    27781 atatgactct atcgacgcgg gcgaccgcta ccaggtgaaa cgcatcaccg tgaaaccggg
    27841 cgagggtttg tcggtacaga tgcaccatca ccgcgcggaa cactgggtag tggtcgcggg
    27901 aacggcaaaa gtcaccattg atggtgatat caaactgctt ggtgaaaacg agtccattta
    27961 tattccgctg ggggcgacgc actgcctgga aaacccgggg aaaattccgc tcgatttaat
    28021 tgaagtgcgc tccggctctt atctcgaaga ggatgatgtg gtgcgcttcg cggatcgcta
    28081 cggacgggtg taaacgtcgc atcaggcgct gtttgtcgga tgcggcgtga acgccttatc
    28141 cgacctacgg ttcgggtttg tagtcctgat aagacgcggc agcgtcgcat caggcaatga
    28201 atgcgaaacc gagatgtaaa taacgacaaa aacaaaattg gccgtttcgg tcagggccaa
    28261 ctattgcctg aaaaagggta acgacatgaa aaaattaacc tgctttaaag cctatgatat
    28321 tcgcgggaaa ttaggcgaag aactgaatga agatatcgcc tggcgcattg gtcgcgccta
    28381 tggcgaattt ctcaaaccga aaaccattgt gttaggcggt gatgtccgcc tcaccagcga
    28441 aaccttaaaa ctggcgctgg cgaaaggttt actggatgcg ggcgtcgacg tgctggatat
    28501 tggtatgtcc ggcaccgaag agatctattt cgccacgttc catctcggcg tggatggcgg
    28561 cattgaagtt actgccagcc ataatccgat ggattataac ggcatgaagc tggtgcgcga
    28621 aggggctcgc ccaatcagcg gtgataccgg actgcgcgac gtccagcgtc tggcagaagc
    28681 caacgacttt cctcccgtcg atgaaaccaa acgcggtcgc tatcagcaaa tcaacctgcg
    28741 tgacgcttac gttgatcacc tgttcggtta tatcaacgtc aaaaacctca cgccgctcaa
    28801 gctggtgatc aactccggga acggcgcagc gggtccagta gtggacgcca ttgaagcccg
    28861 ctttaaagcc ctcggcgcgc ccgtggaatt aatcaaagta cacaacacgc cggacggcaa
    28921 tttccccaac ggtattccta acccgctgct gcctgaatgc cgcgacgaca cccgcaatgc
    28981 ggtcatcaaa cacggcgcgg atatgggcat tgcctttgat ggcgattttg accgctgttt
    29041 tctgtttgac gaaaaagggc agtttatcga gggctactac attgtcggcc tgctggcaga
    29101 agcgttcctc gaaaaaaatc ccggcgcgaa gatcatccac gatccgcgtc tctcctggaa
    29161 caccgttgat gtagtgacgg ccgcaggcgg cacaccggtg atgtcgaaaa ccggacacgc
    29221 ctttattaaa gaacgtatgc gcaaggaaga cgccatctac ggtggcgaaa tgagcgccca
    29281 ccactatttc cgtgatttcg cttactgcga cagcggcatg atcccgtggc tgctggtcgc
    29341 cgaactggtg tgcctgaaag agaaaacgct gggcgaactg gtacgcgacc ggatggcggc
    29401 gtttccggca agcggtgaga tcaacagcaa actggcgcaa cccgttgagg cgattaaccg
    29461 cgtggaacag cattttagcc gtgaggcgct ggcggtggat cgcaccgatg gcatcagcat
    29521 gacctttgcc gactggcgct ttaacctgcg cacctccaac accgaaccgg tggtgcgcct
    29581 gaatgtggaa tcgcgcggtg atgtgccgct gatggaagcg cgaacgcgaa ctctgctggc
    29641 gttgctgaac gagtaatgtc ggatcttccc ttaccccatt gcgggtaagg ggctaataac
    29701 aggaacaacg atgacaaatc taaaaaagcg cgagcgagcg aaaaccaatg catcgttaat
    29761 ctctatggtg caacgctttt cagatatcac catcatgttt gccggactat ggctggtttg
    29821 cgaagtgagc ggactgtcat tcctctacat gcacctgttg gtggcactga ttacgctggt
    29881 ggtgttccag atgttgggcg gcatcaccga tttttatcgc tcatggcgcg gtgttcgggc
    29941 agcgacagaa tttgccctgt tgctacaaaa ctggacctta agcgtgattt tcagcgccgg
    30001 actggtggcg ttcaacaatg atttcgacac gcaactgaaa atctggctgg cgtggtatgg
    30061 gctgaccagc atcggactgg tggtttgccg ttcatgtatt cgcattgggg cgggctggct
    30121 gcgtaatcat ggctataaca agcgcatggt cgcagtggcg ggggatttag ccgccggaca
    30181 aatgctgatg gagagcttcc gtaatcagcc gtggttaggg tttgaagtgg tgggtgtcta
    30241 tcacgaccca aaaccgggcg gcgtttctaa cgactgggcg ggcaatctgc aacagctggt
    30301 agaggatgca aaagcgggca agattcataa cgtctatatc gcgatgcaaa tgtgcgacgg
    30361 cgcacgcgtg aaaaaactgg tccatcaact ggcggacacc acctgttcgg tgttgctgat
    30421 ccccgatgtc tttaccttca acattctcca ttcacgtctt gaagagatga acggcgttcc
    30481 ggtggtgccg ctttacgaca cgccgctttc cggggttaac cgcctgctca aacgtgcgga
    30541 agacattgtg ctggcgacgc ttatcctgct gctgatctcc ccggtgctgt gctgcattgc
    30601 gctggcggta aaagtcagtt caccagggcc ggttattttc cgccagactc gctacggcat
    30661 ggatggcaag ccgatgaaag tgtggaagtt ccgttccatg aaagtgatgg agaacgacaa
    30721 agtggtgacc caggcgacgc agaacgatcc gcgcgtcact aaagtgggga actttctgcg
    30781 ccgcacctcg ctggatgaac tgccgcagtt tatcaatgtg ctgaccgggg ggatgtcgat
    30841 tgtgggtcca cgtccgcacg cggtggcgca taacgaacag tatcgccagc tcattgaagg
    30901 ctacatgctg cgccataaag tgaaaccggg cattaccggc tgggcgcaga ttaacggctg
    30961 gcgcggcgaa accgacacgc tggagaaaat ggaaaaacgc gtcgagttcg accttgagta
    31021 catccgcgaa tggagcgtct ggttcgatat caaaatcgtt ttcctgacgg tattcaaagg
    31081 cttcgttaac aaagcggcat attgatatga gcttacgtga aaaaaccatc agcggcgcga
    31141 agtggtcggc gattgctacg gtgatcatca tcggcctcgg gctggtacag atgaccgtgc
    31201 tggcgcggat tatcgacaac caccagttcg gtttgcttac cgtgtcgctg gtgatcatca
    31261 cgctggcaga tacgctttct gacttcggta tcgctaactc gattattcag cgaaaagaaa
    31321 tcagccacct tgaactcacc acgctgtact ggctgaacgt cgggctgggg cttgtggtgt
    31381 gcgtggcggt gtttttgttg agtgatgcca ttggcgacgt gctgaataac ccggacctgg
    31441 caccgttgat taaaacattg tcgctggcgt ttgtggtgat cccccacggg caacagttcc
    31501 gcgcgttgat gcagaaggag ctggagttca acaaaatcgg catgatcgaa accagcgcgg
    31561 tactggcggg cttcactttt acggtggtta gcgcccattt ctggccgctg gcgatgaccg
    31621 cgatcctcgg ttatctggtc aatagtgcgg tgagaacgct gctatttggc tactttggtc
    31681 gaaaaattta tcgccccggt ctgcatttct cgctggcgtc agttgcgccg aacttacgct
    31741 ttggtgcctg gctgacggtg gacagcatca tcaactatct caacaccaac ctttcaacgc
    31801 tggtgctggc gcgtattctt ggcgcgggcg tggcaggggg atacaacctg gcgtacaacg
    31861 tggccgtcgt accgccgatg aagctgaacc caatcatcac ccgcgtgtta tttccggcat
    31921 tcgccaaaat tcaggacgac accgaaaagc tgcgcgtgaa cttctacaag ctgctgtcgg
    31981 tggtggggat tatcaacttt ccggcgctgc tcgggctgat ggtggtgtcg aataactttg
    32041 taccgctggt ctttggtgag aagtggaaca gtattattcc ggtgctgcaa ttgctgtgtg
    32101 tggtggggct actgcgctcc gtgggtaacc cgattggttc gctgctgatg gcgaaagcgc
    32161 gggtcgatat cagctttaaa ttcaacgtat tcaaaacatt tctgtttatt ccggcgattg
    32221 ttattggtgg gcagatggcg ggcgcaatcg gcgtcacgct cggtttcctg ctggtgcaga
    32281 ttatcaacac cattctgagt tacttcgtga tgattaaacc ggttcttggt tccagttatc
    32341 gccagtacat cctgagtttg tggctgccgt tttatctctc gttgccgacg ctggtggtca
    32401 gttatgcgct gggcattgtg ctgaaagggc aactggcgct ggggatgctg ctggcggtgc
    32461 aaatagccgc gggcgtgctg gcgtttgtgg tgatgattgt gctgtcgcgc catccgctgg
    32521 tggtggaagt gaagcgtcag ttttgtcgca gcgaaaaaat gaaaatgctt ttacgggcgg
    32581 ggtgaatggt tgttttaggc tccggcaata aaaacaacaa cataacctca cctggcatca
    32641 aatttatgag gaaaaaatga aattacttat tctgggcaac cacacttgcg gcaatcgtgg
    32701 cgacagcgcc atcttgcgcg gcttacttga tgccatcaac atcctcaatc cacaagccga
    32761 agtggacgtg atgagccgct atccggtcag ttcttcctgg ctgctcaacc gcccggtaat
    32821 gggcgatccg ctgttcctgc aaatgaaaca acacaacagc gcggcgggcg ttgtcgggcg
    32881 cgttaaaaaa gtcctccgtc gccgctatca acaccaggta ttgctctcac gcgtcaacga
    32941 cactggcaag ctgcgtaata tcgccatcgc ccagggattc accgacttcg tgcgcctgct
    33001 gtcaggttac gacgccatta ttcaggtcgg cggatcgttt tttgtcgatc tctacggcgt
    33061 gccgcagttt gaacacgcgc tttgcacttt catggcgaaa aagccgctgt ttatgattgg
    33121 tcacagcgtc ggtcccttcc aggatgagca atttaaccaa ctggcgaact atgtctttgg
    33181 tcactgcgac gcgctgatcc tgcgcgaatc ggtcagcctt gatctgatga aacgcagcaa
    33241 tatcaccacc gcaaaagtgg aacatggcgt cgataccgca tggctggtcg atcaccacac
    33301 cgaagatttc accgccagct atgccgttca acactggctg gacgttgccg cacaacagaa
    33361 aacagtggca attaccctgc gcgaactggc accgttcgac aaacgtctcg gcaccaccca
    33421 acaagcgtat gaaaaagcct ttgccggggt ggtcaatcgc attctcgatg aagggtatca
    33481 ggtgattgag ctctccacct gtacgggtat cgacagctat aacaaagacg accgcatggt
    33541 ggcgctcaac ctgcgccagc acatcagcga tcctgcccgt taccacgtag tgatggatga
    33601 actcaacgat ctggaaatgg gcaaaattct gggggcctgt gaactcaccg tcggtacgcg
    33661 cctgcactcc gccattatct caatgaactt tgccaccccg gcgattgcca tcaactatga
    33721 acataaatcc gccgggatca tgcagcagct gggactaccg gagatggcga ttgatatccg
    33781 tcatttactg gacggtagac tgcaagcgat ggttgcggat accttaggcc agcttccggc
    33841 actgaacacg cgacttaacg acgccgtcag tcgcgaacgt cagacgggaa tgcagatggt
    33901 gaaatctgtg cttgaacgca tcggggaggc gaaatgaagg tcggcttctt tttactgaaa
    33961 tttccgctgt cgtcggaaac cttcgtcctc aaccaaatta ccgcgtttat tgatatgggg
    34021 tttgaggtgg agattgtcgc gctgcaaaaa ggcgacacac aaaacaccca cgcggcatgg
    34081 acgaaataca accttgccgc cagaacccgc tggttacagg acgaacctac gggcaaagtg
    34141 gcgaaactgc gccaccgtgc cagccagacc ttacgcggaa ttcatcgtaa aaatacctgg
    34201 caggcgctca acctcaaacg ctatggtacc gagtcgcgga acctgatttt gtctgccatt
    34261 tgcggccagg tcgcaacacc attttatgcc gatgtcttta tcgcacattt tggccctgcg
    34321 ggggtaaccg cagcaaaact acgggaactg ggtgtcattc gcggcaaaat cgccactatc
    34381 ttccacggta ttgatatctc cagtcgggag gtgctcaacc actacactcc cgaatatcaa
    34441 caactgtttc gccgtggcga cctgatgtta ccgattagcg atctgtgggc cggaaggctg
    34501 caaaaaatgg gctgcccgag ggaaaaaatt gccgtatcgc gcatgggtgt agacatgacg
    34561 cgctttagcc cgcgtccggt gaaagcgccc gcaacaccgc tggagattat ttccgtcgca
    34621 cgcttaaccg agaaaaaagg tctgcatgtg gcgattgaag cctgccgtca gttgaaagag
    34681 cagggcgtgg catttcgcta tcgcatcctc ggcattggcc cgtgggaacg acgcctgcgc
    34741 actctcatcg aacaatatca actggaagat gtggtagaga tgccgggctt taaaccgagc
    34801 cacgaagtga aagcgatgct cgacgacgcg gatgtcttcc tgttgccatc ggtaacgggt
    34861 gcggacggcg atatggaagg cattccggtg gcgctgatgg aggcgatggc ggtcggcatt
    34921 ccggtggttt ctactctgca tagcggaata ccggaactgg tggaggctga caaatccggc
    34981 tggctggtgc ctgagaacga tgctcgcgca ctggcgcaac gactggcggc gtttagccaa
    35041 ctggacaccg acgaattggc tccggttatc aaacgtgcgc gcgaaaaagt cgaacacgat
    35101 tttaaccagc aggtgattaa tcgagaactc gccagcttgt tgcaggcttt atagaggaga
    35161 acgtatgcca tttaaaaaac tctcccgacg caccttcctg acggcaagct cggcgcttgc
    35221 cttccttcat acccctttcg ctcgcgcact tcctgcccga caaagcgtta acattaacga
    35281 ctacaacgct cacgactgga tcgcctcatt taaacaagcc ttcagcgaag ggcaaacagt
    35341 cgtcgtacct gctggattcg tttgtgacaa tatcaacacc ggcatcttca ttcctcccgg
    35401 taaaacgtta cacatcctcg gaagcctgcg cggcaacggc agagggcgat ttgtcttaca
    35461 ggacggcagc caggtgacag gggagaaggg cggcagtatg cataacatca ccctggatgt
    35521 gcgtggttct gattgcacca tcaaagggct ggcgatgagc ggctttggcc cggtaacgca
    35581 gatttatatc ggcggcaaaa acaaacgggt catgcgcaac ctgaccatcg ataacctgac
    35641 cgttagccac gctaattacg ccatcttacg ccagggattt cataaccaga ttatcggtgc
    35701 caacatcacc aactgtaagt tcagcgactt acaaggcgac gccattgaat ggaacgtggc
    35761 aattaacgac agtgatattt tgatctccga ccacatcatc gagcgcatca actgtactaa
    35821 cggcaaaatc aactggggca ttggcatagg tcttgcggga agtacttatg ataacaacta
    35881 cccggaagac caggcagtga aaaactttgt cgtggcgaat atcacgggat cggattgtcg
    35941 gcaactgatc catgttgaaa atggtaaaca ttttgttatt cgtaatatca aagcccgcaa
    36001 tatcacgccg gatttcagta agaaagcagg tattgataac gcgacggttg ctatttacgg
    36061 ttgtgacaat ttcgtgattg ataatattga aatgattaat agcgccggga tgttaatcgg
    36121 ttacggggta attaaaggca aatatctctc gataccgcaa aatttccgag tgaataatat
    36181 tcaactggat aatacccacc ttgcttacaa attgcgcggt atccaaatct ccgccgggaa
    36241 taccgtctcc tttgtggcgc tgactaacat tgagatgaag cgtgcgtcat tggagttaca
    36301 caacaagccg caacaccttt ttatgcgtaa tatcaaggtg atgcaggaat cctcagttgg
    36361 accagcattg agtatgaact ttgacatgcg caaagacgtt cgcggcgtgt ttatggcgaa
    36421 aaaagaaaca ctactgtctc ttgcaaatgt tcatgcggtg aatgaaagag ggcaaagctc
    36481 cgtcgatatc gacaggatta atcaccatat tgttaatgtg gaaaagatta actttagatt
    36541 gccggaacgg agggagtaga tttgcgacca ttcctggaaa aatggagcca tacttaggaa
    36601 caatgctact gcaatccaca acgaagcggc gtaacatcac cagtaattca gtaatcaatt
    36661 cagggtaatt gatgctggcg aaaaaaatcg aacaagctat aattcagcaa ccattttaca
    36721 ggtggatgaa ataatgacga atttaaaagc agttattcct gtagcgggtc ttgggatgca
    36781 tatgttgcct gccactaagg cgatacccaa agagatgcta ccgatcgtcg acaagccaat
    36841 gattcagtac attgttgacg agattgtggc tgcagggatc aaagaaatcc tcctggtaac
    36901 tcacgcgtcc aagaacgcgg tcgaaaacca cttcgacaca tcttatgaat tagaatctct
    36961 ccttgaactg cgcgtgaagc gtcaactgct ggcggaagta cagtcaattt gcccgccggg
    37021 cgtgacaatt atgaacgtgc gtcagggcga acctttaggt ttaggccact ccattttatg
    37081 tgcacgaccc gccattggtg acaatccatt tgtcgtggtg ctgccagacg ttgtgatcga
    37141 cgatgccagc gccgacccgc tgcgctacaa ccttgcagcc atgattgcgc gcttcaacga
    37201 aacgggccgt agccaggtac tggcaaaacg tatgccgggc gatctctctg aatactccgt
    37261 cattcagacc aaagagccgc tggaccgtga aggcaaagtc agccgcattg ttgaatttat
    37321 cgaaaaaccg gatcagccgc agacgctgga ctcagacatc atggccgtgg gccgttatgt
    37381 gctttctgcc gatatttggc cggaactgga acgtactcag cctggtgcat gggggcgtat
    37441 tcagctgact gatgccatcg ctgaactggc gaaaaaacag tccgttgatg ccatgctgat
    37501 gacaggtgac agctacgact gcggtaaaaa aatgggttat atgcaggcat ttgtgaagta
    37561 tggactacgc aacctcaaag aaggggcgaa gttccgtaaa gggattgaga agctgttaag
    37621 cgaataatga aaatctgacc ggatgtaacg gttgataaga aaattataac ggcagtgaag
    37681 attcgtggcg aaagtaattt gttgcgaata ttcctgccgt tgttttatat aaacaatcag
    37741 aataacaacg agttagcaat aggattttcg tcaaagtttt ccaggatttt ccttgtttcc
    37801 agagcggatt ggtaagacaa ttagcgtttg aatttttcgg gtttagcgcg agtgggtaac
    37861 gctcgtcaca tcgtaggcat gcatgcagtg ctctggtagc tgtaaagcca ggggcggtag
    37921 cgtgtgttaa tacctctatt aatcaaactg agagccgctt atttcacagc atgctctgaa
    37981 gtaatacgga ataaattaag tgaaaatact tgttactggt ggcgcaggat ttattggttc
    38041 tgctgtagtt cgtcacatta taaataatac gcaggatagt gttgttaatg tcgataaatt
    38101 aacgtacgcc ggaaacctgg aatcacttgc tgatgtttct gattctgaac gctatgtttt
    38161 tgaacatgcg gatatttgcg atgctactgc aatggcgcgg atttttgctc tgcatcagcc
    38221 ggatgcagtg atgcacctgg ctgctgaaag ccatgtggat cgttcaatta caggccctgc
    38281 ggcatttatt gaaaccaata ttgttggtac ttatgtcctt ttggaagcgg ctcgcaatta
    38341 ctggtctgct cttgatggcg acaagaaaaa tagcttccgt tttcatcata tttctactga
    38401 cgaagtttat ggcgatctgc ctcatcctga cgaagtaaat aataaagaag aattaccctt
    38461 atttactgag acaacagctt acgcaccaag cagcccttat tctgcatcaa aagcgtccag
    38521 cgatcattta gtccgcgcgt ggaaacgtac ctatggttta ccgaccattg tgactaattg
    38581 ctctaacaat tatggtcctt atcattttcc ggaaaaattg attccattgg ttattctgaa
    38641 tgctctggaa ggtaaagcat tacctattta cggcaaaggg gatcaaattc gcgactggtt
    38701 gtatgttgaa gatcatgcgc gtgcgttata taccgtcgta accgaaggta aagcgggtga
    38761 aacttataac attggtggac acaacgaaaa gaaaaacata gatgtagtgc tcactatttg
    38821 tgatttgctg gatgagattg taccgaaaga gaaatcttat cgtgagcaaa tcacttatgt
    38881 tgccgatcgt ccgggacacg atcgccgtta tgccattgat gctgagaaga ttggtcgcga
    38941 attgggatgg aaaccacagg aaacgtttga gagcgggatt cggaagacag tggaatggta
    39001 cctgtccaat acaaaatggg tcgaaaatgt gaaaagtggt gcctatcagt catggattgc
    39061 acagaactat gagggccgcc agtaatgaat atcctccttt ttggcaaaac agggcaggta
    39121 ggttgggaac tacagcgtgc tctggcacct ttgggtaatt tgattgctct tgatgttcac
    39181 tctactgatt attgcggtga ttttagtaat cctgacggtg tagctgaaac cataagaagc
    39241 attcggcctg atattattgt caacgcagcc gctcacaccg cagtagacaa agcagaatca
    39301 gaaccggagt ttgcacaatt acttaacgca acaagtgtcg aagcgattgc gaaagcagca
    39361 aatgaagttg gagcttgggt tatccattac tcgactgatt acgtcttctc tggaaatggc
    39421 gatatgccat ggctggagac ggatacaacc gcaccactaa atgtttacgg tgaaaccaag
    39481 ttagccggag aaaaagcgtt acaggaatat tgcgcaaagc atcttatttt ccggaccagc
    39541 tgggtctatg caggaaaagg aaataacttc gccaaaacga tgttacgtct ggcaaaagag
    39601 cgtgaagaat tagcggttat taacgatcag tttggtgcgc caacaggtgc tgaactgctg
    39661 gctgattgta cagcacatgc cattcgtgtc gcactgaata aaccggatgt cgcaggcttg
    39721 taccatttgg tagccagtgg taccacaacc tggtacgatt atgctgcgct ggtttttgaa
    39781 gaggcgcgca aagcaggcat tcccctttca ctcaacaagc tcaacgcagt accaacaact
    39841 gcctacccta caccagctcg tcgtccacat aactctcgcc ttaatacaga aaaatttcag
    39901 cagaactttg cgcttgtctt gcctgactgg caggttggtg tgaaacgaat gctcaacgaa
    39961 ttatttacga ctacagcaat ttaatagttt ttgcatcttg ttcgtgatgg tggagcaaga
    40021 tgaattaaaa ggaatgatga aatgaaaacg cgtaagggta ttattttagc gggtggttct
    40081 ggtactcgtc tttatcctgt gactatggca gtcagtaaac agctgctacc gatttatgat
    40141 aaaccgatga tctattatcc gctctctaca ctgatgttgg cgggtattcg cgatattttg
    40201 attatcagta cgccacagga tactcctcgt tttcaacaac tgctgggtga cggtagccag
    40261 tgggggctga atcttcagta caaagtgcaa gcgagtccgg atggtcttgc gcaggcattt
    40321 attatcggtg aagagtttat tggtggtgat gattgtgctt tgattcttgg tgataatatc
    40381 ttttacggtc acgatctgcc gaagttaatg gatgccgctg ttaacaaaga aagtggtgca
    40441 acggtatttg cctatcacgt aaatgatcct gaacgctacg gtgtcgttga gtttgataaa
    40501 aacggtacgg caataagcct ggaagaaaaa ccgctacaac caaaaagtaa ttatgcggta
    40561 accggacttt atttctatga caatgatgtt gtagaaatgg cgaaaaacct taagccttct
    40621 gcccgtggcg aactggaaat taccgatatt aaccgtattt atatggagca gggacgtttg
    40681 tctgtcgcta tgatggggcg tggttatgcc tggttggata ctggtacaca tcaaagtctt
    40741 attgaagcaa gtaacttcat tgccaccatt gaagagcgtc agggattaaa ggtatcttgc
    40801 ccggaagaga ttgcttaccg taaagggttt attggagaaa
//
LOCUS       DAZKHC020000034        40426 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZKHC020000034 DAZKHC020000000
VERSION     DAZKHC020000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 40426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.034"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..270)
                     /locus_tag="ACHOSM_RS10485"
                     /old_locus_tag="pgaptmp_002097"
     CDS             complement(<1..270)
                     /locus_tag="ACHOSM_RS10485"
                     /old_locus_tag="pgaptmp_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HGU4558614.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVG"
     gene            complement(430..642)
                     /gene="rpmE"
                     /locus_tag="ACHOSM_RS10490"
                     /old_locus_tag="pgaptmp_002098"
     CDS             complement(430..642)
                     /gene="rpmE"
                     /locus_tag="ACHOSM_RS10490"
                     /old_locus_tag="pgaptmp_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HGU4558615.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            846..3044
                     /gene="priA"
                     /locus_tag="ACHOSM_RS10495"
                     /old_locus_tag="pgaptmp_002099"
     CDS             846..3044
                     /gene="priA"
                     /locus_tag="ACHOSM_RS10495"
                     /old_locus_tag="pgaptmp_002099"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGU4558616.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3200..4225
                     /gene="cytR"
                     /locus_tag="ACHOSM_RS10500"
                     /old_locus_tag="pgaptmp_002100"
     CDS             3200..4225
                     /gene="cytR"
                     /locus_tag="ACHOSM_RS10500"
                     /old_locus_tag="pgaptmp_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HGU4558617.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4317..5276
                     /gene="ftsN"
                     /locus_tag="ACHOSM_RS10505"
                     /old_locus_tag="pgaptmp_002101"
     CDS             4317..5276
                     /gene="ftsN"
                     /locus_tag="ACHOSM_RS10505"
                     /old_locus_tag="pgaptmp_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HGU4558618.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQEQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5369..5899
                     /gene="hslV"
                     /locus_tag="ACHOSM_RS10510"
                     /old_locus_tag="pgaptmp_002102"
     CDS             5369..5899
                     /gene="hslV"
                     /locus_tag="ACHOSM_RS10510"
                     /old_locus_tag="pgaptmp_002102"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HGU4558619.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5909..7240
                     /gene="hslU"
                     /locus_tag="ACHOSM_RS10515"
                     /old_locus_tag="pgaptmp_002103"
     CDS             5909..7240
                     /gene="hslU"
                     /locus_tag="ACHOSM_RS10515"
                     /old_locus_tag="pgaptmp_002103"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HGU4558620.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAETAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7307..8233
                     /gene="menA"
                     /locus_tag="ACHOSM_RS10520"
                     /old_locus_tag="pgaptmp_002104"
     CDS             7307..8233
                     /gene="menA"
                     /locus_tag="ACHOSM_RS10520"
                     /old_locus_tag="pgaptmp_002104"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HGU4558621.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8326..8811
                     /gene="rraA"
                     /locus_tag="ACHOSM_RS10525"
                     /old_locus_tag="pgaptmp_002105"
     CDS             8326..8811
                     /gene="rraA"
                     /locus_tag="ACHOSM_RS10525"
                     /old_locus_tag="pgaptmp_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HGU4558622.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGTAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8896..9141)
                     /gene="zapB"
                     /locus_tag="ACHOSM_RS10530"
                     /old_locus_tag="pgaptmp_002106"
     CDS             complement(8896..9141)
                     /gene="zapB"
                     /locus_tag="ACHOSM_RS10530"
                     /old_locus_tag="pgaptmp_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HGU4558623.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9567..10412
                     /gene="glpF"
                     /locus_tag="ACHOSM_RS10535"
                     /old_locus_tag="pgaptmp_002107"
     CDS             9567..10412
                     /gene="glpF"
                     /locus_tag="ACHOSM_RS10535"
                     /old_locus_tag="pgaptmp_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HGU4558624.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNSVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10435..11943
                     /gene="glpK"
                     /locus_tag="ACHOSM_RS10540"
                     /old_locus_tag="pgaptmp_002108"
     CDS             10435..11943
                     /gene="glpK"
                     /locus_tag="ACHOSM_RS10540"
                     /old_locus_tag="pgaptmp_002108"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGU4558625.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNAGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12078..13088
                     /gene="glpX"
                     /locus_tag="ACHOSM_RS10545"
                     /old_locus_tag="pgaptmp_002109"
     CDS             12078..13088
                     /gene="glpX"
                     /locus_tag="ACHOSM_RS10545"
                     /old_locus_tag="pgaptmp_002109"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU4558626.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMDIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13185..13931
                     /gene="fpr"
                     /locus_tag="ACHOSM_RS10550"
                     /old_locus_tag="pgaptmp_002110"
     CDS             13185..13931
                     /gene="fpr"
                     /locus_tag="ACHOSM_RS10550"
                     /old_locus_tag="pgaptmp_002110"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HGU4558627.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13936..14364)
                     /gene="uspD"
                     /locus_tag="ACHOSM_RS10555"
                     /old_locus_tag="pgaptmp_002111"
     CDS             complement(13936..14364)
                     /gene="uspD"
                     /locus_tag="ACHOSM_RS10555"
                     /old_locus_tag="pgaptmp_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HGU4558628.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14391..14690)
                     /gene="yiiS"
                     /locus_tag="ACHOSM_RS10560"
                     /old_locus_tag="pgaptmp_002112"
     CDS             complement(14391..14690)
                     /gene="yiiS"
                     /locus_tag="ACHOSM_RS10560"
                     /old_locus_tag="pgaptmp_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HGU4558629.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAATASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14902..15342)
                     /gene="yiiR"
                     /locus_tag="ACHOSM_RS10565"
                     /old_locus_tag="pgaptmp_002113"
     CDS             complement(14902..15342)
                     /gene="yiiR"
                     /locus_tag="ACHOSM_RS10565"
                     /old_locus_tag="pgaptmp_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4558630.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15443..16042
                     /gene="yiiQ"
                     /locus_tag="ACHOSM_RS10570"
                     /old_locus_tag="pgaptmp_002114"
     CDS             15443..16042
                     /gene="yiiQ"
                     /locus_tag="ACHOSM_RS10570"
                     /old_locus_tag="pgaptmp_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HGU4558631.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPFMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16150..16917
                     /gene="tpiA"
                     /locus_tag="ACHOSM_RS10575"
                     /old_locus_tag="pgaptmp_002115"
     CDS             16150..16917
                     /gene="tpiA"
                     /locus_tag="ACHOSM_RS10575"
                     /old_locus_tag="pgaptmp_002115"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGU4558632.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16972..17727)
                     /gene="cdh"
                     /locus_tag="ACHOSM_RS10580"
                     /old_locus_tag="pgaptmp_002116"
     CDS             complement(16972..17727)
                     /gene="cdh"
                     /locus_tag="ACHOSM_RS10580"
                     /old_locus_tag="pgaptmp_002116"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HGU4558633.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17834..18823)
                     /gene="sbp"
                     /locus_tag="ACHOSM_RS10585"
                     /old_locus_tag="pgaptmp_002117"
     CDS             complement(17834..18823)
                     /gene="sbp"
                     /locus_tag="ACHOSM_RS10585"
                     /old_locus_tag="pgaptmp_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HGU4558634.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19144..20106)
                     /gene="pfkA"
                     /locus_tag="ACHOSM_RS10590"
                     /old_locus_tag="pgaptmp_002118"
     CDS             complement(19144..20106)
                     /gene="pfkA"
                     /locus_tag="ACHOSM_RS10590"
                     /old_locus_tag="pgaptmp_002118"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGU4558635.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20287..21189)
                     /gene="fieF"
                     /locus_tag="ACHOSM_RS10595"
                     /old_locus_tag="pgaptmp_002119"
     CDS             complement(20287..21189)
                     /gene="fieF"
                     /locus_tag="ACHOSM_RS10595"
                     /old_locus_tag="pgaptmp_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HGU4558636.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21338..21838)
                     /gene="cpxP"
                     /locus_tag="ACHOSM_RS10600"
                     /old_locus_tag="pgaptmp_002120"
     CDS             complement(21338..21838)
                     /gene="cpxP"
                     /locus_tag="ACHOSM_RS10600"
                     /old_locus_tag="pgaptmp_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HGU4558637.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21988..22686
                     /gene="cpxR"
                     /locus_tag="ACHOSM_RS10605"
                     /old_locus_tag="pgaptmp_002121"
     CDS             21988..22686
                     /gene="cpxR"
                     /locus_tag="ACHOSM_RS10605"
                     /old_locus_tag="pgaptmp_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HGU4558638.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22683..24056
                     /gene="cpxA"
                     /locus_tag="ACHOSM_RS10610"
                     /old_locus_tag="pgaptmp_002122"
     CDS             22683..24056
                     /gene="cpxA"
                     /locus_tag="ACHOSM_RS10610"
                     /old_locus_tag="pgaptmp_002122"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HGU4558639.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24162..24836)
                     /gene="yiiM"
                     /locus_tag="ACHOSM_RS10615"
                     /old_locus_tag="pgaptmp_002123"
     CDS             complement(24162..24836)
                     /gene="yiiM"
                     /locus_tag="ACHOSM_RS10615"
                     /old_locus_tag="pgaptmp_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HGU4558640.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24985..25968)
                     /gene="kdgT"
                     /locus_tag="ACHOSM_RS10620"
                     /old_locus_tag="pgaptmp_002124"
     CDS             complement(24985..25968)
                     /gene="kdgT"
                     /locus_tag="ACHOSM_RS10620"
                     /old_locus_tag="pgaptmp_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HGU4558641.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFVSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGSLLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26228..26848)
                     /gene="sodA"
                     /locus_tag="ACHOSM_RS10625"
                     /old_locus_tag="pgaptmp_002125"
     CDS             complement(26228..26848)
                     /gene="sodA"
                     /locus_tag="ACHOSM_RS10625"
                     /old_locus_tag="pgaptmp_002125"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HGU4558642.1"
                     /translation="MSYTLPSLPYAYDALEPHFDEQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27133..28167
                     /gene="rhaT"
                     /locus_tag="ACHOSM_RS10630"
                     /old_locus_tag="pgaptmp_002126"
     CDS             27133..28167
                     /gene="rhaT"
                     /locus_tag="ACHOSM_RS10630"
                     /old_locus_tag="pgaptmp_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HGU4558643.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSTLGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28164..29012)
                     /gene="rhaR"
                     /locus_tag="ACHOSM_RS10635"
                     /old_locus_tag="pgaptmp_002127"
     CDS             complement(28164..29012)
                     /gene="rhaR"
                     /locus_tag="ACHOSM_RS10635"
                     /old_locus_tag="pgaptmp_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HGU4558644.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(29086..29922)
                     /gene="rhaS"
                     /locus_tag="ACHOSM_RS10640"
                     /old_locus_tag="pgaptmp_002128"
     CDS             complement(29086..29922)
                     /gene="rhaS"
                     /locus_tag="ACHOSM_RS10640"
                     /old_locus_tag="pgaptmp_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HGU4558645.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30210..31679
                     /gene="rhaB"
                     /locus_tag="ACHOSM_RS10645"
                     /old_locus_tag="pgaptmp_002129"
     CDS             30210..31679
                     /gene="rhaB"
                     /locus_tag="ACHOSM_RS10645"
                     /old_locus_tag="pgaptmp_002129"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HGU4558646.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWDVDSLESAIRLGLNMVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTLFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31676..32935
                     /gene="rhaA"
                     /locus_tag="ACHOSM_RS10650"
                     /old_locus_tag="pgaptmp_002130"
     CDS             31676..32935
                     /gene="rhaA"
                     /locus_tag="ACHOSM_RS10650"
                     /old_locus_tag="pgaptmp_002130"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HGU4558647.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEDSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYASSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPTGSEWLESVRAYEKAILSQRG"
     gene            33177..34001
                     /gene="rhaD"
                     /locus_tag="ACHOSM_RS10655"
                     /old_locus_tag="pgaptmp_002131"
     CDS             33177..34001
                     /gene="rhaD"
                     /locus_tag="ACHOSM_RS10655"
                     /old_locus_tag="pgaptmp_002131"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HGU4558648.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            34011..34325
                     /gene="rhaM"
                     /locus_tag="ACHOSM_RS10660"
                     /old_locus_tag="pgaptmp_002132"
     CDS             34011..34325
                     /gene="rhaM"
                     /locus_tag="ACHOSM_RS10660"
                     /old_locus_tag="pgaptmp_002132"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HGU4558649.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34626..35072
                     /gene="frvA"
                     /locus_tag="ACHOSM_RS10665"
                     /old_locus_tag="pgaptmp_002133"
     CDS             34626..35072
                     /gene="frvA"
                     /locus_tag="ACHOSM_RS10665"
                     /old_locus_tag="pgaptmp_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HGU4558650.1"
                     /translation="MAVLTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSRQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQQQQGDTDQVLALLNQTLSS"
     gene            35083..36534
                     /gene="frvB"
                     /locus_tag="ACHOSM_RS10670"
                     /old_locus_tag="pgaptmp_002134"
     CDS             35083..36534
                     /gene="frvB"
                     /locus_tag="ACHOSM_RS10670"
                     /old_locus_tag="pgaptmp_002134"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HGU4558651.1"
                     /translation="MDSSLRIVAITNCPAGIAHTYMAAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36524..37594
                     /gene="frvX"
                     /locus_tag="ACHOSM_RS10675"
                     /old_locus_tag="pgaptmp_002135"
     CDS             36524..37594
                     /gene="frvX"
                     /locus_tag="ACHOSM_RS10675"
                     /old_locus_tag="pgaptmp_002135"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4558652.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37594..39342
                     /gene="frvR"
                     /locus_tag="ACHOSM_RS10680"
                     /old_locus_tag="pgaptmp_002136"
     CDS             37594..39342
                     /gene="frvR"
                     /locus_tag="ACHOSM_RS10680"
                     /old_locus_tag="pgaptmp_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HGU4558653.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEDKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTRLVENI
                     TGHLLRCLAAPVWIAEHRQSTMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVLNHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(39392..40373)
                     /gene="yiiG"
                     /locus_tag="ACHOSM_RS10685"
                     /old_locus_tag="pgaptmp_002137"
                     /pseudo
     CDS             complement(39392..40373)
                     /gene="yiiG"
                     /locus_tag="ACHOSM_RS10685"
                     /old_locus_tag="pgaptmp_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
BASE COUNT         9815 a        10488 c        10467 g         9656 t
ORIGIN      
        1 ccccaccggc gcgggcgttt ttgtccggta actgctgtag gtacgggaga ggatgaacgg
       61 cagcggggcg ggcagggcga tgtcggtttc accgggaagg accttcgcac cgagcagcgg
      121 attgaccgga tggccggacg tcaccccgcc ggggcacacc gaacaggcca cgccggtggg
      181 ggcaccaatg cgcaccccgg ctgaaccctg gacaatgcta ccgccatact gcgtcatgtc
      241 gccctgacgc gccgccggtt ttccgctcat cgtaaaatcc ttttgaaata aataagaacc
      301 agaagcctag cgccagtcaa caacaggaaa tgataatgag ttaacacccc ggaatttttc
      361 agaatttata aaaggcaaat tacaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga
      421 aatccggtct tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc
      481 accggtagca acatcacgct gtttgccagt gaagaacggg tggcacttgc tgcacacgtc
      541 gaggttcagg tcatgaccaa cggtggagcg gattttcatt acgttaccgc aagagcagct
      601 agcagtaatt tcttcgtatt tcgggtgaat atcttttttc atgggaaaac ctcggtttaa
      661 ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc gatgttaaaa gtatagcttc
      721 aatacgatca tttcgtacga aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac
      781 tgatccgcac tcttcttacg gcaatgtgta tactaaccca ccaaatttca agtcaggatg
      841 atgctatgcc cgttgcccac gttgccttgc ccgttccgct tcctcgtacc tttgactatc
      901 tgctgccaga aggcatgacg gttaaagctg ggtgtcgcgt gcgcgtgccg tttggcaaac
      961 agcaggagcg catcgggatt gtggtatcag ttagcgatgc cagcgaactg ccgctcaatg
     1021 agctaaaagc ggtagtcgaa gtgctggata gtgagccggt gtttactcac tccgtctggc
     1081 gattgctgct atgggcggca gattactatc atcatccgat tggcgatgtg ctgtttcatg
     1141 ccttgccgat tttactacgc caggggcggc ctgcggcgaa cgcgccgatg tggtactggt
     1201 ttgccactga acaaggtcag gcggtggatc tgaacagcct gaaacgctca cccaagcaac
     1261 aacaggcgct ggcagcgtta cggcaaggca aaatctggcg cgaccaggtt gccacgctcg
     1321 aatttaatga tgccgcgttg caggcgctgc gcaaaaaagg tctgtgtgat ttagcaagtg
     1381 aaacaccaga gtttagcgac tggcgaacga actatgccgt ttctggtgag cggttgcgat
     1441 tgaataccga acaggccacc gccgttggcg caattcatag cgcggcagat actttttctg
     1501 cctggctgct ggcgggcgtt accggttccg gtaaaacgga ggtttatctc agtgtactgg
     1561 aaaacgtgct cgctcagggc aaacaggcgc tggtgatggt gccggaaatc ggcctgacac
     1621 cgcaaactat cgcccgtttt cgtgaacgtt ttaatgcccc cgtggaagtt ctgcattccg
     1681 gcctgaacga cagcgagcgg ctttcggcgt ggttgaaagc gaaaaatggc gaggcggcga
     1741 ttgtgatcgg cacccgctcc gcgctgttta cgccgtttaa aaatctcggc gtgattgtca
     1801 ttgatgaaga gcacgacagc tcctacaagc agcaggaagg ctggcgctat catgcccgcg
     1861 acctggcggt gtatcgtgcg catagcgagc aaatcccgat tattcttggc tccgcaacgc
     1921 ccgcgctgga aacgttatgc aacgtccagc agaaaaaata ccgcctgctg cgcctgaccc
     1981 gtcgggcagg gaatgcgcgt ccggcaattc aacatgtgct ggatttaaaa ggtcagaagg
     2041 tgcaggcagg tctggctccg gcgttaatca cccgtatgcg ccagcattta caggccaaca
     2101 atcaggttat cctctttctt aatcgtcggg gttttgcgcc tgcgctgctg tgccacgact
     2161 gcggctggat agccgaatgc ccacgttgcg atcactacta cacgctgcat caggcgcaac
     2221 agcatttgcg ctgtcaccac tgtgacagtc agcgtccggt gccgcgccag tgcccttcct
     2281 gcggttccac gcacctggtc cccgtggggc tgggcaccga acagcttgaa cagacgcttg
     2341 cgccgatgtt ccccggcgtg cccatttcac gtatcgaccg cgataccacc agccgcaaag
     2401 gggcgctgga acagcaactg gcggaagtgc atcgcggcgg cgcgaggatc ttgattggca
     2461 cgcaaatgct ggcgaaaggt caccactttc cggatgtgac gctggtggcg ttactggatg
     2521 tggacggcgc gctgttttct gccgattttc gctcggcaga acgtttcgcc cagctttaca
     2581 cccaggtcgc cggtcgtgcc gggcgtgcgg gtaaacaggg cgaagtggtg ctgcaaacgc
     2641 accatccgga acatcctctg ttgcaaacgt tgctctataa aggctacgac gcctttgccg
     2701 aacaggcgct ggctgagcga cgcatgatgc agctgccgcc gtggaccagc catgtgattg
     2761 tgcgtgcgga agatcataac aatcagcatg cgccgctgtt cctgcaacaa ctgcgtaatc
     2821 tgatcctttc cagcccgctg gcagacgaca aactgtgggt tctcggcccg gttccggctc
     2881 tggcacctaa gcgtggtggc cgctggcgct ggcagatctt gttgcagcac ccttcccgcg
     2941 tgcgcttgca acacatcatt agtggtacgc tggcgcttat taatacaata ccggattccc
     3001 gcaaggtgaa atgggtgttg gatgtcgatc ccatcgaagg ctaatgcgca aacattgcga
     3061 ggcggatcga aaaattcaat attcatcaca cttttcatga aaattctgta accgttttca
     3121 cgcgctatct gctaaaaatg ttgccgatgt gaagtaaaca tggatgtagt acgcctgacg
     3181 tgccaggcga ggagtgagtg tgaaagcgaa gaagcaggaa actgccgcga ccatgaaaga
     3241 cgttgccctc aaggcaaaag tctctacagc gactgtctcc cgagcattaa tgaatcccga
     3301 taaagtctcc caggccaccc gtaatcgggt tgaaaaagcg gcccgggaag tgggttattt
     3361 accgcaacct atggggcgca acgtcaagcg taatgaatcc cgcaccattc tggtgattgt
     3421 cccggatatc tgcgatccct tctttagcga aattattcgc ggtatcgaag ttacggcggc
     3481 aaatcacgga tatctggtgc tgattggcga ctgtgcgcat caaaatcagc aggaaaaaac
     3541 ctttatcgat ttgatcatca ccaagcaaat tgatggcatg ttgctgctgg gctcaaggct
     3601 gccgtttgat gccagcattg aggaacagcg taatctgccg ccgatggtga tggcgaacga
     3661 atttgcaccg gaactggagc tgcctaccgt tcatatcgac aatctgaccg ccgcatttga
     3721 tgcagtaaat tatttatatg agcaagggca taaacggatt ggctgtatag ccggtcccga
     3781 agagatgcca ctgtgtcact accgcctgca aggctatgtt caggcgctgc gtcgctgcgg
     3841 cattatggtt gatccgcaat acatcgcccg tggcgacttc accttcgaag ccggaagcaa
     3901 agcgatgcag cagctgcttg atcttccaca accgcctact gctgtcttct gccatagcga
     3961 tgtgatggcg ctcggcgcac tttctcaggc aaaacgccag gggctgaaag tcccggaaga
     4021 cctttccata atcggttttg ataacatcga cctgacgcaa ttttgtgatc cgccgctgac
     4081 aaccatcgcg cagccgcgtt acgaaatcgg tcgggaagct atgctgttat tgcttgatca
     4141 aatgcagggg caacacgttg gcagtggctc tcgtttaatg gactgcgaac ttatcatccg
     4201 gggatcaaca cgcgcgttac cttaaagtaa acctttaaga ctgccgtgac tggtcaaagg
     4261 cccgccgctt aagtaacatg gcgggctgac gaacgaataa atacagcgaa acgatagtgg
     4321 cacaacgaga ttatgtacgc cgcagccaac cggcaccttc gcggcgaaaa aagagcacct
     4381 cacggaaaaa gcaacgaaat ctgcctgcgg tttctcccgc tatggtcgct attgctgccg
     4441 ccgttcttgt gacctttatc ggtggtctgt acttcattac gcatcacaag aaagaagagt
     4501 ctgagacgct gcaaagccag aaagtgaccg gtaacggact accaccaaaa ccagaagaac
     4561 gctggcgcta cattaaagag ctggaaagtc gccagccggg tgtgcgtgcg cccacagaac
     4621 cttctgccgg tggtgaagtg aaaacgccgg agcaactgac accagaacaa cgtcagcttc
     4681 ttgaacaaat gcaggctgat atgcgccagc agccaacgca gctggttgaa gttccgtgga
     4741 atgaacagac gccggaacag cgtcagcaaa cgctacaacg ccagcgtcag gcgcagcaat
     4801 tagcggagca gcaacgcctg gctcagcagt cgcgtacgac tgaacaaagc tggcagcagc
     4861 agacgcgtac gtcgcaagcc gcgccggtac aggagcaacc gcgccagtct aaaccggctt
     4921 ctactcagca gccgtaccag gatctgctgc aaacccctgc gcacacgacc gcgcagtcga
     4981 agccgcagca agctgcgccg gttgctcgtg ccgctgacgc gccaaaaccg acggcggaga
     5041 aaaaagacga acgccgctgg atggtgcagt gcggttcgtt cagaggcgcg gaacaggcag
     5101 agacggtacg tgctcagctg gcgttcgaag gatttgactc gaaaatcacc accaacaatg
     5161 gctggaatcg tgtggtcatt ggcccggtga aaggcaaaga gaacgcagac agcaccctca
     5221 atcggttgaa gatggcgggt catacaaact gcattcggct cgccgccggg ggttgaaacc
     5281 ctcaaaatcc cccccatcta taattgcatt atgccccgta cttttgtacg gggtttgtac
     5341 tctgtattcg taaccaaggg gtcagctcgt gacaactata gtaagcgtac gccgtaacgg
     5401 ccatgtggtc atcgctggtg atggtcaggc cacgttgggc aataccgtaa tgaaaggcaa
     5461 cgtgaaaaag gtccgccgtc tgtacaacga caaagtcatc gcgggctttg cgggcggtac
     5521 tgcggatgct tttacgctgt tcgaactgtt tgaacgtaaa ctggaaatgc atcagggcca
     5581 tctggtcaaa gccgccgttg agctggcgaa agactggcgt accgatcgca tgctgcgcaa
     5641 acttgaagca ctgctggcag tcgcggatga aacagcatcg cttatcatca ccggtaacgg
     5701 tgacgtggtg cagccagaaa acgatcttat tgctatcggc tccggcggcc cttacgccca
     5761 ggctgcggcg cgcgcgctgt tagaaaacac tgaacttagc gcccgtgaaa ttgctgaaaa
     5821 ggcgttggat attgcaggcg acatttgcat ctataccaac catttccaca ccatcgaaga
     5881 attaagctac aaagcgtaag gatctcccat gtctgaaatg accccacgcg aaatcgtcag
     5941 cgaattggat aagcacatca tcggccagga caacgccaag cgttctgtgg cgattgctct
     6001 gcgtaaccgc tggcgtcgca tgcagctcaa cgaagagctg cgccatgaag tgaccccgaa
     6061 aaatatcctg atgatcggcc cgaccggtgt cggtaaaact gaaatcgccc gtcgtctggc
     6121 taagctggcg aatgcgccgt tcatcaaagt tgaagcgacc aaattcaccg aagtgggcta
     6181 tgtcggtaag gaagtggatt ctattattcg cgatctgacc gatgccgccg tgaaaatggt
     6241 acgcgtccag gctatcgaga aaaaccgtta tcgcgctgaa gaactggcag aagaacgtat
     6301 tctcgacgtg ctgatcccac ctgctaaaaa caactgggga cagaccgaac agcagcagga
     6361 accgtccgct gctcgtcagg cattccgcaa aaaactgcgt gaaggccagc ttgatgacaa
     6421 agaaatcgag atcgatcttg ccgcagcacc gatgggcgtt gaaattatgg ctcctccagg
     6481 catggaagag atgaccagcc agctgcagtc catgttccag aacctgggcg gccagaagca
     6541 aaaagcgcgt aagctgaaaa tcaaagacgc catgaagctg ctgattgaag aagaagcggc
     6601 gaaactggtg aacccggaag agctgaagca agacgctatc gacgctgttg agcagcacgg
     6661 gatcgtgttt atcgacgaaa tcgacaaaat ctgtaagcgc ggcgagtctt ccggtccgga
     6721 tgtttctcgt gaaggcgttc agcgtgacct gctgccgctg gtagaaggtt gcaccgtttc
     6781 caccaaacac gggatggtca aaactgacca cattctgttt atcgcttctg gcgcgttcca
     6841 gattgcgaaa ccgtctgacc tgatcccgga actgcaaggt cggctgccaa tccgcgttga
     6901 actgcaggca ctgaccacca gcgacttcga gcgtattctg accgagccga atgcctctat
     6961 caccgtgcag tacaaagcac tgatggcgac tgaaggcgta aatatcgagt ttaccgactc
     7021 cggtattaag cgcatcgcgg aaacggcatg gcaggtgaac gaatctaccg aaaacatcgg
     7081 tgctcgtcgt ttacacactg ttctggagcg tttaatggaa gagatttcct acgacgccag
     7141 cgatttaagc ggtcaaaata tcactattga cgcagattat gtgagcaaac atctggatgc
     7201 gttggtggca gatgaagatc tgagccgttt tatcctataa tcgcgttcaa tcattttcat
     7261 cattgtttga tggggctgaa aggccccatt tttattggcg cgtattatga ctgaacaaca
     7321 aattagccga actcaggcgt ggctggaaag tttacgacct aaaaccctcc ccctcgcctt
     7381 tgctgcaatt atcgtcggga cggcgctggc atggtggcaa ggtcacttcg atccgctggt
     7441 cgccctgctg gcactaatta ccgccgggct attacagatc ctttctaacc tcgccaatga
     7501 ttacggcgat gcggtaaaag gcagcgataa acctgaccgc attgggccgc tacgcggcat
     7561 gcaaaaaggg gtcattaccc agcaagagat gaaacgggcg ctcattatta ccgtcgtgct
     7621 catctgtctc tccgggctgg cactggttgc agtggcatgc catacgctgg ccgattttgt
     7681 cggtttcctg attcttggcg ggttgtcgat cattgccgct atcacctaca ccgtgggcaa
     7741 tcgtccttat ggttatatcg gtctgggtga tatttccgta ctggttttct ttggctggtt
     7801 gagtgtcatg gggagctggt atttacaggc tcatacattg attccggcac tgatccttcc
     7861 ggcgaccgca tgcggcctgc tggcaacggc agtactgaat attaataacc tgcgtgatat
     7921 caatagcgac cgcgaaaatg gcaaaaacac gctggtggtg cgcttaggtg aagtgaacgc
     7981 gcgtcgttat catgcctgcc tgctgatggg ctcgctggtg tgtctggcgc tgtttaatct
     8041 cttttcgctg catagcctgt ggggctggct gttcctgctg gcggcaccat tactggtgaa
     8101 gcaagcccgt tatgtgatgc gggaaatgga cccggtggcg atgcgaccaa tgctggaacg
     8161 tactgtcaag ggagcgttac tgactaacct gctgtttgtt ttagggatat tcctaagcca
     8221 gtgggcagca taactgacaa atatcaatta acaattgatg attttgccaa cagcccacat
     8281 agcgcgatat actgaaaatt ctcgcagcaa ctgaatgtta agcctatgaa atacgatact
     8341 tccgagcttt gtgacatcta tcaagaagat gttaacgtcg tggaaccgct gttctccaac
     8401 tttggcggac gggcgtcgtt tggcggacaa ataatcacgg taaaatgttt cgaggacaac
     8461 gggttgctgt acgatctgct cgaacagaat ggccgtggtc gtgttcttgt cgtcgatggc
     8521 ggtggttctg ttcgtcgcgc actggtcgat gctgaactgg cgcgtctggc agtacaaaat
     8581 gaatgggaag gtctggtcat ttacggcgcg gtgcgtcagg tagatgacct ggaagagttg
     8641 gatatcggca tccaggcgat ggcggcaatt ccggttggta ccgctggcga aggcattggc
     8701 gaaagcgatg tccgcgtcaa ttttggcggt gtcaccttct tctccggcga ccatctttat
     8761 gccgacaata ccgggattat tctttcagaa gatccgctgg atattgaatg ataagaaagg
     8821 caccgcaagg tgcctttttt ctgcgttacc tgttggccta cacagtaaag aaattacgcg
     8881 aaagatgaag cgtaatcaga cctcttccat gcgacccagc agggcctgca gacgttcctg
     8941 ccagccgttc tgctgttctt tcagatggtt gttctcacgc tccagctctt cgcgctgatg
     9001 ctgggcattt tgaacttcct gcgacagtga gttgtttttt tctttcagct cttcgatttc
     9061 catctgcaac agagtgatgg tatcaatcgc ctgctgtact tttgcttcca gtttctcaaa
     9121 cacttctaat gacattgtca tacctctcct gaattgcaag gcgttgatgg ataaaaaatc
     9181 ctcgtcccga ttaccggtga cgccttaata aatacgagcg cactttagtt agctccgatt
     9241 gtatgaagcc gcgccatcgc tgtccagcgg cacgccttgc agattacggt ttgccacact
     9301 tttcatcctt ctcctggtga cataatccac atcaatcgaa aatgttaata aatttgttgc
     9361 gcgaatgatc taacaaacat gcatcatgta caatcagatg gaataaatgg cgcgataacg
     9421 ctcattttat gacgaagcac acacatttta agttcgatat ttctcgtttt tgctcgttaa
     9481 cgataagttt acagcatgcc tacaagcatc gtggaggtcc gtgactttca cgcatacaac
     9541 aaacattaac tcttcaggat ccgattatga gtcaaacatc aaccttgaaa ggccagtgca
     9601 ttgctgaatt cctcggtacc gggttgttga ttttcttcgg tgtgggttgc gttgcagcac
     9661 taaaagtcgc tggtgcgtct tttggtcagt gggaaatcag tgtcatttgg ggactggggg
     9721 tggcaatggc catctacctg accgcagggg tttccggcgc gcatcttaat cccgctgtta
     9781 ccattgcatt gtggctgttt gcctgtttcg acaagcgcaa agttattcct tttatcgttt
     9841 cacaagttgc cggcgctttc tgcgctgcgg ctttagttta cgggctttac tacaatttat
     9901 ttttcgactt cgagcagact catcacattg ttcgcggcag cgttgaaagt gttgatctgg
     9961 ctggcacttt ctctacttac cctaatcctc atatcaattt tgtgcaggct ttcgcagttg
    10021 agatggtgat taccgctatt ctgatggggc tgatcctggc gttaacggac gatggcaaca
    10081 gtgtaccacg cggccctttg gctcccttgc tgattggtct actgattgcg gtcattggcg
    10141 catctatggg cccattgacg ggttttgcca tgaacccagc gcgtgacttc ggtccgaaag
    10201 tctttgcctg gctggcgggc tggggcaatg tcgcctttac cggcggcaga gacattcctt
    10261 acttcctggt gccgcttttt ggccctatcg ttggcgcgat tgtaggtgca tttgcctacc
    10321 gcaaactgat tggtcgccat ttgccttgcg atatctgtgt tgtggaagaa aaggaaacca
    10381 caactccttc agaacaaaaa gcttcgctgt aatatgacta cgggacaatt aaacatgact
    10441 gaaaaaaaat atatcgttgc gctcgaccag ggcaccacca gctcccgcgc ggtcgtaatg
    10501 gatcacgatg ccaatatcat tagcgtgtcg cagcgcgaat ttgagcaaat ctacccaaaa
    10561 ccaggttggg tagaacacga cccaatggaa atctgggcca cccaaagctc cacgctggta
    10621 gaagtgctgg cgaaagccga tatcagttcc gatcaaattg cagctatcgg tattacgaac
    10681 cagcgtgaaa ccactattgt ctgggaaaaa gaaaccggca agcctatcta taacgccatt
    10741 gtctggcagt gccgtcgtac cgcagaaatc tgcgagcatt taaaacgtga cggtttagaa
    10801 gattatatcc gcagcaatgc cggtctggtg attgacccgt acttttctgg caccaaagtg
    10861 aagtggatcc tcgaccatgt ggaaggctct cgcgagcgtg cacgtcgtgg tgaattgctg
    10921 tttggtacgg ttgatacgtg gcttatctgg aaaatgactc agggccgtgt ccatgtgacc
    10981 gattacacca acgcctctcg taccatgttg ttcaacatcc ataccctgga ctgggacgac
    11041 aaaatgctgg aagtgctgga tattccgcgc gagatgctgc cagaagtgcg tcgttcttcc
    11101 gaagtatacg gtcagactaa cattggcggc aaaggcggca cgcgtattcc aatctccggg
    11161 atcgccggtg accagcaggc cgcgctgttt ggtcagttgt gcgtgaaaga agggatggcg
    11221 aagaacacct atggcactgg ctgctttatg ctgatgaaca ctggcgagaa agcggtgaaa
    11281 tcagaaaacg gcctgctgac caccatcgcc tgcggcccga ctggcgaagt gaattatgcg
    11341 ttggaaggtg cggtgtttat ggcaggcgca tccattcagt ggctgcgcga tgagatgaag
    11401 ttgattaacg acgcctacga ttccgaatat ttcgccacca aagtgcaaaa caccaatggt
    11461 gtgtatgtgg ttccggcatt taccgggctg ggtgcgccgt actgggaccc gtatgcgcgc
    11521 ggggcgattt tcggtctgac tcgtggggtg aacgctaacc acattatacg cgcgacgctg
    11581 gagtctattg cttatcagac gcgtgacgtg ctggaagcga tgcaggccga ctctggtatc
    11641 cgtctgcacg ccctgcgcgt ggatggtggc gcagtagcaa acaatttcct gatgcagttc
    11701 cagtccgata ttctcggcac ccgcgttgag cgcccggaag tgcgcgaagt caccgcattg
    11761 ggtgcggcct atctcgcagg cctggcggtt ggcttctggc agaacctcga cgagctgcaa
    11821 gagaaagcgg tgattgagcg cgagttccgt ccaggcatcg aaaccactga gcgtaattac
    11881 cgttacgcag gctggaaaaa agcggttaaa cgcgcgatgg cgtgggaaga acacgacgaa
    11941 taatgtaaat gccgaatgaa gcgtttatgc cgcatccggt agtcccgaaa cgtgcggggg
    12001 caaccccgca cacatcaata atccctccct tcccctgtgc tacacttcgc gccattcctt
    12061 actgcttaga gtttgctatg agacgagaac ttgccatcga attttcccgc gtcaccgaat
    12121 cagcggcgct ggctggctac aaatggttag gacgcggcga taaaaacacc gcggacggcg
    12181 cggcggtaaa cgccatgcgt attatgctca accaggtcaa cattgacggc accatcgtca
    12241 ttggtgaagg tgaaatcgac gaagcaccga tgctctacat tggtgaaaaa gtcggtactg
    12301 gtcgcggcga cgcggtagat attgctgttg atccgattga aggcacgcgc atgacggcga
    12361 tgggccaggc taacgcgctg gcggtgctgg cagtaggcga taaaggctgc ttcctcaatg
    12421 cgccggatat gtatatggag aagctgattg tcgggccggg agccaaaggc accattgatc
    12481 tgaacctgcc gctggcggat aacctgcgca atgtagcggc ggcgctcggc aaaccgttga
    12541 gcgaactgac ggtaacgatt ctggctaaac cacgccacga tgccgttatc gctgaaatgc
    12601 agcaactcgg cgtacgcgta tttgctattc cggacggcga cgttgcggcc tcaattctca
    12661 cctgtatgcc agacagcgaa gttgacgtgc tgtacggtat tggtggcgcg ccggaaggcg
    12721 tggtttctgc ggcggtgatc cgcgcattag atggcgacat gaacggtcgt ctgctggcgc
    12781 gtcatgacgt caaaggcgac aacgaagaga atcgtcgcat tggcgagcag gagctggcac
    12841 gctgcaaagc gatggacatc gaagccggta aagtattgcg tcttggcgat atggcgcgca
    12901 gcgataacgt catcttctct gccaccggta ttaccaaagg cgatctgctg gaaggtatta
    12961 gccgcaaagg caatatcgcg actaccgaaa cgctgctgat ccgcggcaag tcacgcacca
    13021 ttcgccgcat tcagtccatc cactatctgg atcgcaaaga cccggaaatg caggtgcaca
    13081 ttctctgagt gatttgatcg attgagcctt ccagtccttc gggactggaa tttttttgtt
    13141 cggagaacga agataaggca agtcaatcaa aacaggagaa aaacatggct gattgggtaa
    13201 caggcaaagt cactaaagtg cagaactgga ccgacgccct gtttagtctc accgttcacg
    13261 cccccgtgct tccgtttacc gccgggcaat tcaccaagct tggccttgaa atcgacggcg
    13321 aacgcgtcca gcgcgcctac tcctatgtta actcgcccga taatcccgat ctggagtttt
    13381 acctggtcac cgtccccgat ggcaaattaa gcccacgatt ggcggcactg aaaccgggcg
    13441 atgaagtgca ggtggttagc gaagcagctg gcttctttgt tctggatgaa gtaccggact
    13501 gcgaaacgct atggatgctg gcaaccggta cagcgattgg cccttattta tcgattctgc
    13561 aactaggcaa agatttagat cgcttcaaaa atctggtcct ggttcacgcc gcacgttatg
    13621 ccgccgactt aagctatttg ccactgatgc aagaactgga aaaacgctac gaagggaaac
    13681 tgcgcattca gacggtggtc agtcgggaaa cggcagcggg gtcgctcacc gggcggatac
    13741 cggcgttaat tgaaagtggg gaactggaaa gcgcgattgg cctgccgatg aataaagaaa
    13801 ccagccatgt gatgctgtgc ggcaatccac agatggtgcg cgatacacaa cagttgctga
    13861 aagagacccg gcagatgacg aaacatttac gtcgccgacc gggccatatg acagcggagc
    13921 attactggta agcggttact tatcgataaa cggcacgatg agcaaatccg cactcatctt
    13981 attgatcatc ccgcgatatg ccggcatcaa acggttgata aatgagtgat gatgaccaca
    14041 gacaaggagg tcgcactgct ctttttgcat aatttccagc agtgtttccg gcatttctcc
    14101 gcgttcaata cgcagttttg tcttcggcca ttgaatattt ttcgtcagtt tatacagctt
    14161 gttatccgac ttattcttca acaattgaag aatatcttct gttgcaggga agtagatacc
    14221 cggatacaac tcgcttaagc catcatcaat atgaattaac gtcaggtgag cgtcattatg
    14281 tctggcgagc tccagagctt tattcaccag taaggcatct tcttcattcc cggaaattgc
    14341 cacgccaatg tgtttataag ccatgtttaa ctccttctaa agcaactcca tcaagctaac
    14401 gaacgcagtg ataactcaaa aataatcatc tcggcctggc atgagaaagt gaaggccgca
    14461 tcaagctcaa cttgcccctc cagatcctta atcttgtagg ccacgctggc cccttcatct
    14521 gcagaggatg cagtggcggc aagctctttc agtttggtca taaaagactc tgcttcttcg
    14581 cgggtggcaa aaaagcgcga aaacttactg gtacagttgt cgttatcaat caccgtaccg
    14641 atatcaatcg cacatccttt agtactgcat ttatctacga catctttcat agggcacctc
    14701 tgtatattcg cctctctgtc gattacgact taagaataat gcgccgttaa gggaagaaca
    14761 aacaagtaat gatcttatgg attaccattt tttttgatat tgcttaaggt gaagagttca
    14821 gaaggatttt ccgggtattg tcatttgcat cgtggaaata acaatcccgg aaaaaaatag
    14881 cagtaagaga tatattaaag gttaattact tgatttattg tcggctttat acttcacatc
    14941 ctgagtatct ttaccatatt tattttcgcc ttgtgtgcca acaaacgcac caagatcgat
    15001 aagcatcatc accagaatca acgtcgggac aaaacgcccc accgcccatt gccagacacc
    15061 cggtaaaatc gcccagttac ccgccagcag catccacgcc acaatcatca gaaatgccca
    15121 tgcgccggaa cgcccgcgat catgcaagcg cttaacagtt actgccgctg ttggccaaag
    15181 caagcacaca aggcaaaacg ccgcggtctg aatatcgagt aaatttttac ccgccagtga
    15241 gaaaagcacc agcatgcctg cgaaccacag gcctatccaa atccagaaat cacggcgtcc
    15301 aatacgccct ttaaatgaga ataaccattg ctgtatggtc atgtaagttc cttgatggtt
    15361 gtcttttcca ggattctacc cttttgacaa gggcgacaga tatcgtttta atcggagcca
    15421 gtcataacaa aaggtactgt taatgaagcc agggtgtacg ctgttttttc tcttatgttc
    15481 tgcattaacc gttacaacaa cggcgcatgc acaaacacca gatacggcaa cgaccgcgcc
    15541 ttatctgctg gctggagccc ctactttcga tctctccatc agccagtttc gagaagactt
    15601 taacagccag aatcctagcc tgccactgaa cgaatttcgt gccatcgaca gcagtcccga
    15661 caaagccaat ctcactcgtg ctgcaagtaa aattaacgag aacttgtatg cttctacagc
    15721 gctggagcgc ggtaccttaa aaatcaaaag cattcaaatg acctggctac ccatccaggg
    15781 gccagagcaa aaagcagcga aagcgaaagc tcaggaatac atggcagcag tgatccgcac
    15841 actcacccca tttatgacca aaacacaaag ccagaaaaaa ctgcagtcgc tactaacggc
    15901 ggggaaaaac aaacgttatt acaccgagac agaaggtgca ctgcgttatg ttgtcgcgga
    15961 caacggcgaa aaggggctga ccttcgctgt tgaaccgatt aagctggcgc tatctgaatc
    16021 gcttgaaggt ttgaataaat gacaaaaagc aaagcctttg tgccgatgaa tctctatact
    16081 gtttcacaga cctgctgccc tgcggggcgg ccatcttcct ttattcgctt ataagcgtgg
    16141 agaattaaaa tgcgacatcc tttagtgatg ggtaactgga aactgaacgg cagccgccac
    16201 atggttcacg agctggtttc taacctgcgt aaagagctgg caggtgttgc tggctgtgcg
    16261 gttgcaatcg caccaccgga aatgtacatc gatatggcga agcgcgaagc tgaaggcagc
    16321 cacatcatgc tgggtgcgca aaacgtggac ctgaacctgt ccggcgcatt caccggtgaa
    16381 acttctgccg ctatgctgaa agacatcggc gctcagtaca tcatcatcgg ccactctgaa
    16441 cgtcgtactt accacaaaga gtctgacgaa ctgatcgcga aaaaattcgc ggtgctgaaa
    16501 gagcagggcc tgactccggt tctgtgcatc ggtgaaaccg aagctgaaaa cgaagcgggc
    16561 aaaactgaag aagtttgcgc acgtcagatc gacgcggtac tgaaaactca gggtgctgcg
    16621 gcattcgaag gtgcggttat cgcttacgaa cctgtatggg caatcggtac tggcaaatct
    16681 gcaactccgg ctcaggcaca ggctgttcac aaattcatcc gtgaccacat cgctaaagtt
    16741 gacgctaaca tcgctgaaca agtgatcatt cagtacggcg gctctgtaaa cgcgtctaac
    16801 gctgcagaac tgtttgctca gccagatatc gacggcgcgc tggttggcgg tgcttctctg
    16861 aaagctgacg ctttcgcagt gatcgttaaa gctgcagaag cggctaaaca ggcttaagtc
    16921 tgacaggtgc cggatttcat atccggcact tactttcctt aactcttcgc cttaacgcaa
    16981 aatctcacac tgatgatcct gaatttcctc ggctgaagca cggttaagcg tcagtagatt
    17041 tcgttgtgtc gccagcaata caaatgagtt atcactctgc cgtaccatcg ccagcccgta
    17101 gcttcccata tgttcccgcg cctcaggtac ttcttctgcc agcatcataa atgggctgcg
    17161 ttgtaccagt tcgctttccg ttacccgacg cgccaggtat tcatgcccgc gcaaaccacc
    17221 tggcagtggc aaccagcggc tgctgatgtt cgccagattg ttatccagtt gttcgcgcac
    17281 atcaggacga atacaagaga tatgaatatg aaaatggttt tgcgtacgcc cggtgcggga
    17341 gttgatcgcc aaagaaaccg cgcgatcggg aaccggctgg ccgtattttt tgctcataaa
    17401 atcacgcgcc tgccaggcca accaaaagaa gttcggcgtt gaaggatcgg tcaacaaagg
    17461 actttcagta ccgttaatac gatacgttgg catcaacaga tattgcagtg gaccattaag
    17521 atcttttaaa accacgtatc cggcattggg tttgacttcc gcacatggcg aaggattttg
    17581 attttgctgc tgattgggca aacattcctc aaggacaatc ttacgtaatg tatccgactc
    17641 ttcaccggtt aatttccagt aaccaatacc ggcagccaca acggcgataa ctatcatcac
    17701 caaaaaaaga agacccgctt ttttcatctt tttttccctg tacctcaaag agatgcaagg
    17761 gtaacgcaaa atcgtgacaa ataaaaaacc cggtcgcgag aaaccaccgg gtttgataat
    17821 tatcctgggg aaatcagcgt ttgctgatct gatcgaacgt accgccgtta gcaaaatgct
    17881 ctttttgcgc tttcgtccag ccgccgaact cttcatcaat ggtgaataac ttcagctttg
    17941 gaaacgcatt ttcgtacttt ttcgctactt cagcgtcgcg cggacggtag tagtttttcg
    18001 cggcaatttc ctgaccttct ggcgagtaga gatatttcag gtaggcttcc gccacctctt
    18061 tggtgccttt tttctcgacc actttatcga ccaccgacac ggttggctct gcgaggatag
    18121 actcactcgg cgtgacgatt tcgaatttat ctttccccag ttcattcgct gccagcagag
    18181 cttcgttttc ccaggcgatc agtacatcgc caatcccgcg ctcgacaaag gtgttagtgg
    18241 agccgcgcgc gccagaatcc agaacttcga cgtttttata cagtgcccga acaaaatcct
    18301 gtgcttttgc ctgatcgttg ttgttgtgat gcagcgcgta gccccaggca gccaggtagt
    18361 tccagcgtgc gccaccagag cttttcggat taggcgtgat caccgaaaca cccggtttaa
    18421 tcagatcgtt ccagtcatgg atctgcttcg gattgccctt acgcaccagg aaaacaatgg
    18481 tggaagtgta cggtgcggag ttatccggca gacgtttgat ccactcttta tcaatccgcc
    18541 cgcgttccgc aatcgcgtcc acgtcatagg ccagagccag cgtgacaaca tcagcttcaa
    18601 taccgttgat taccgacgtc gcttgtttac ctgaaccacc gtgtgactga cgaatcacca
    18661 cgttatcacc agtttgctgt ttccagtggg cgctgaatgc cttgttgtac tgttcgtaca
    18721 attcgcgcgt tggatcatat gaaacgttaa gaagctgaat atcctttgcc ataacgctgg
    18781 ttgccgccag caaaaatgtt aaccctacgc cccacttgtt catcgcccga ctctcttatg
    18841 ttgtgttgtg atgagcaaag cgtgccagaa ggttaaccaa acattaaaga ataaaaaaaa
    18901 gattggctat aacttgcggg tatatgttga gggattaaaa aggcggaaga aatccgcctc
    18961 atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc ttatccggcc
    19021 tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata agcgaagcgc
    19081 atcaggcatt tttgcttctg tcatcggttt caggctaaag ggatctgcct ttttccgaaa
    19141 tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac gcttcatgtt
    19201 ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac ctacacaacg
    19261 accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg aagccagaat
    19321 acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag ttgcgcgggt
    19381 ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac acatatgttc
    19441 ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga tttcgtttac
    19501 caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc caccggcaat
    19561 ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca cggaaatacg
    19621 ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg tgctcagcgc
    19681 agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag tgcccggcag
    19741 accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg aaccgtcacc
    19801 gccgataacc accagcgcgt cgataccacg ttttttcagg ttttcgatag ccacggcgcg
    19861 gatgttctcg tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac cgccacggtt
    19921 gatcatgtca gaaacgctgt aacggtctag ctgtaccata cggtcttcat acagacccag
    19981 atagccgtca taaatgccca ttacttccag accttctgtc agcgcagaac gaacaacccc
    20041 gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac cgattttctt
    20101 aatcatgact acctctgaac tttggaatgc aaaataaaat ctgttgccgg aagtcttctt
    20161 gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc tgctgaattg
    20221 attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg ctctctttta
    20281 tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac aggggtcctg
    20341 atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta cctgatccgc
    20401 caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa tctgaataaa
    20461 gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac ccggccagga
    20521 agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat ccagtaatga
    20581 ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc cgattcccaa
    20641 tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg ccagcagaat
    20701 tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca ccgcctggct
    20761 ttgcgtccgc cgcaccaccc aacgctgaaa cgagacaagg ataatcgtac aaattagcgc
    20821 cacaattgtc acgataaccc cgacgcctgg atctgtcatc ggtgttggag atatcagatg
    20881 ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac tttgcgccag
    20941 cgccgcgagg gactcagctt taccgtgacc aaacgagtga ttatcgtcgg caggttgcag
    21001 ggaatatcgc accaccaata aattcgtcaa cgacgcgccg atatccacca gcgaatccac
    21061 cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt taatcagcaa
    21121 tagcagcgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc gtccataaga
    21181 ttgattcata aatactcccg ctatcaactg acgccagtat aacggaagca aatcatctgc
    21241 aatgcattaa gcagcaggca aattgaggat aaaaaaaacc cccacatcat gtgggggaag
    21301 acagggatgg tgtctatggc aaggaaaaca gggtttacta ctgggaacgt gagttgctac
    21361 tactcaatag cttcaacgat gaactttttt gccattgcgt cacgtcacgc aactgctcca
    21421 ttcgttgttg atgtttctcg tttaaaaccg cttgctgctc cggcgttaac aggcgataca
    21481 tttggttgcg gacttttgcc atctcaacct gacgagcaat ttgctcattc gccatttttt
    21541 ctgcctgtgc gcgcacagcg ttttcatcaa aattttctgc ggtgacaagg cgatgcattg
    21601 tctccagttc gctaacatta acaggaggct gttcgtgccg ggcctgttgc ataagatctc
    21661 gcatctgctg acgctgatgt tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac
    21721 tgcgctgcgt aagttcttca cccggatgcc agttatcgcc tgaaccgact tcagcagcgt
    21781 ggcttaatga actgactgcc agcgttgagg ccatgacggc agcggtaact atgcgcatca
    21841 tttgctccca aaatctttct gtcgcgattc aacgatagag agtttacgat tcaggctgca
    21901 aacatgcgtc agggggtgta aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt
    21961 aatttctgcc tcggaggtat ttaaacaatg aataaaatcc tgttagttga tgatgaccga
    22021 gagctgactt ccctattaaa ggagctgctc gagatggaag gcttcaacgt gattgttgcc
    22081 cacgatgggg aacaggcgct tgatcttctg gacgacagca ttgatttact tttgcttgac
    22141 gtaatgatgc cgaagaaaaa tggtatcgac acattaaaag cacttcgcca gacacaccag
    22201 acgcctgtca ttatgttgac ggcgcgcggc agtgaacttg atcgcgttct cggccttgag
    22261 ctgggcgcag atgactatct cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt
    22321 cgcgcgatcc tgcgccgttc gcactggagc gagcaacagc aaaacaacga caacggttca
    22381 ccgacactgg aagttgatgc cttagtgctg aatccaggcc gtcaggaagc cagcttcgac
    22441 gggcaaacgc tggagttaac cggtactgag tttaccctgc tctatttgct ggcacagcat
    22501 ctgggtcagg tggtttcccg tgaacattta agccaggaag tgttgggcaa acgcctgacg
    22561 cctttcgacc gcgctattga tatgcacatt tccaacctgc gtcgtaaact gccggatcgt
    22621 aaagatggtc acccgtggtt taaaaccttg cgtggtcgcg gctatctgat ggtttctgct
    22681 tcatgatagg cagcttaacc gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg
    22741 tgttgatgtt ggttttgatg ttacccaagc tcgattcacg ccagatgacc gagcttctgg
    22801 atagcgaaca gcgtcagggg ctgatgattg agcagcatgt cgaagcggag ctggcgaacg
    22861 atccgcccaa cgatttaatg tggtggcggc gtctgttccg ggcgattgat aagtgggcac
    22921 cgccaggaca gcgtttgtta ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca
    22981 gcgaaatgca gatcattcgt aactttattg gtcaggccga taacgccgat catccgcaga
    23041 agaaaaagta tggccgcgtg gaactggtcg gtccgttctc cgtacgtgat ggcgaagata
    23101 attaccaact ttatctgatt cgtccggcca gcagttctca atccgatttc attaacttac
    23161 tgtttgaccg cccgctatta ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt
    23221 tgtggttggc ctggagtctg gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag
    23281 ttgcccaggg aaacttacgc cagcacccgg aactggaagc ggggccacag gaattccttg
    23341 ccgcaggtgc cagttttaac cagatggtca ccgcgctgga gcgtatgatg acctcccagc
    23401 agcgtctgct ttctgatatc tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg
    23461 gtacggcgtt actgcgccgt cgtagcggtg aaagcaagga actggagcgt attgaaaccg
    23521 aagcgcaacg tctggacagc atgatcaacg atctgttggt gatgtcacgt aatcagcaaa
    23581 aaaacgcgct ggttagcgaa accatcaaag ccaaccagtt gtggagtgaa gtgctggata
    23641 acgcggcgtt cgaagccgag caaatgggca agtcgttgac agttaacttc ccgcctgggc
    23701 cgtggccgct gtacggcaat ccgaacgccc tggaaagtgc gctggaaaac attgttcgta
    23761 atgctctgcg ttattcccat acgaagattg aagtgggctt tgcggtagat aaagacggta
    23821 tcaccattac ggtggacgac gatggtcctg gcgttagccc ggaagatcgc gaacagattt
    23881 tccgtccgtt ctatcggacc gatgaagcac gcgatcgtga atctggcggt acaggattgg
    23941 ggctggcgat tgttgaaacc gccattcagc agcatcgtgg ctgggtgaag gcagaagaca
    24001 gcccgctggg cggtttacgg ctggtgattt ggttgccgct gtataagcgg agttaaactc
    24061 cgcatttgta ggcaggataa ggcgtttacg ccgcatccgg catttgagca ggatgcctga
    24121 tgcgacgctg atagcgtctt atcaggccta cactccgggg gttattttcc ccacaaccgg
    24181 cgggaaaaat cttcgatctt gccgctcagt cggcgctttt gcatcgtccg cgtccagctt
    24241 ttcgataacc cggcggcgga gagtaaacgg tgatattgat cgtcatcaaa cggcatatgc
    24301 catgcgatgg cggcagcctc ctgcacggtc acatcgctga cacgggaaac caactccagc
    24361 ggcgcgtccg cagacacttt ccctggcgct atcacgctat acaaccaccc cactttaccc
    24421 gtgttttgca tcaactgcgc tatatcgctg atatcaaaat ggtaattgag tttatagcaa
    24481 ggcgagcgcg gctggctgac ctgaattaat gcctctcccc agcggaaaat atcgcccata
    24541 taaacattac tttccgtgag accgtcggtt gagagatttt cgccaaacgc aggcgcaaca
    24601 aacaactccg cctgttccgg aaattcccgc gcccagtaga gatagtgctc acgaggataa
    24661 tgacacagcg ctctgtctgg cccgccgtga acctttttct ccgcctgctc gtcaccttcc
    24721 agccccagct ccgtcagcat caactcacca tcaacctgga ttttggcaat tgcgctgggt
    24781 ttgccttcgg gataagcctg aatcttgcct gtgtatacat caaccggata tcgcatctgt
    24841 tccctctcca cgagaaattt ccccattaga ccatatttat tttatgtcag gcgtgctgtt
    24901 ttcgcggtaa gtatccaggc aggaactcaa tagcaaaccg gaagaacgtt agttcatccg
    24961 gctgtggaga ttaagaaagc aggtttattt caccgtacct aaaatttcga ttttcgctgc
    25021 tctggctttg actttacgtg accagataga ggtaagaatt ggcaccagaa tcgaagtcac
    25081 aatgaccgcc gtcgctacca gcgaagttgc tgccggagcc atcggtttaa acgcaggcac
    25141 catttctgca atcagcacag gtgtcgctac cgcggcccct gcggaactgg aagcggcaat
    25201 tccggcagtg ccatcgccac cgccaatcaa tttatcggcg ataatcaacg gaataccggt
    25261 cacgataatt actgccacac ccaacaggct ccccagcaaa ccagtctggg caatcacagt
    25321 caaatcaatg gtattgccca gcgcgaaggc aaagaatgga atcagcgttt gcaccgcttt
    25381 gctgacaaat tctcgtaatt cagggtcaag gttccccagg gcaaagccca ccaggaacgg
    25441 taatactgcg ccgacgaaaa catgcggttc aaacgaggca atccctgcag tgcccagaat
    25501 aatcatcgtc atgagcggcc cggactccaa cgacatcaac acaaatgccc cagcttcttc
    25561 ttttgtgccg tactgctgca tgatggaagc gtaaagtccg ccattggtca tatccatcgc
    25621 cgccaccagc gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg
    25681 aataatgcgc gaggcaattg ccgcaaccac ccacgcgacg gcaatttttg ttaccaccag
    25741 cgtaccggat ttacgcaata ccgttcccgt cgcgcttaat tttattgacg cccccatgca
    25801 aaaaaaccac accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc
    25861 aaaatatttc cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac
    25921 gagcatcatc ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg
    25981 tcggcatatt tttaagggct gatgcgtcat ttctcaatgg agcatcagtg ttgatggaaa
    26041 cgaagataga cattcattta atcaataaaa gtgatcaaaa ccacaccaat tgaaacactg
    26101 ttttattttt atcggatcat tgtttttatt ttgaaattga tcacaaaaaa accaccgccg
    26161 ttggcgatgg ttcatttttt aagctgatat gcggcgtaaa cgcctcattg cagctggcgg
    26221 caaatgatta ttttttcgcc gcgaaacgcg ccgcagcttc gtcccagttc accacgttcc
    26281 agaactcttt gatgtagtcc gggcggcggt tctggaattt caggtagtaa gcatgttccc
    26341 acacatccaa gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg
    26401 gagaatcctg gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg
    26461 cccagccgga accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa
    26521 cggagccgaa gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt
    26581 tcagaccttt ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg
    26641 ttttcttgtc tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg
    26701 caaattctgg caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt
    26761 tggtgtggtg gatttccatg gtctgctcat cgaagtgcgg ttccagggca tcgtaagcat
    26821 acggcaggga tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg
    26881 ttaatgccgc gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat
    26941 caataccgta cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa
    27001 atcacccact taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt
    27061 taatcgacag cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat
    27121 gaggataaga agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg
    27181 gccagtgcag cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc
    27241 atgtggtcag tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg
    27301 ttactaccgg atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt
    27361 tttctgttcg gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat
    27421 ctcggcatgt cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg
    27481 atgacgccaa ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg
    27541 acgttgctcg gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag
    27601 ttgaaagagc gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg
    27661 ctggcggtga tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa
    27721 ccgatgcatg aagccgctgc cgcacttggc gtcgatccac tgtatgtcgc tctgccaagc
    27781 tatgtcatca tcatgggcgg cggcgcgatc atcaacctcg gtttctgttt tattcgtctg
    27841 gcaaaagtga aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt
    27901 cacaatgtgt tactctcgac actgggcggg ttgatgtggt atctgcaatt ctttttctat
    27961 gcttggggcc acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg
    28021 agtttctatg tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaatgca
    28081 ggacgccgtc cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac
    28141 atcgtcggca tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg
    28201 ggcgtcatcc cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca
    28261 cattcggtcg aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga
    28321 cacactctga cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc
    28381 tgacgcaaaa cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga
    28441 cttttcaggc tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt
    28501 ggcggcaacg aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac
    28561 agcaactcag ccatttcgtt agcaaacggc acatgctgac tactttcatg ctcaagctga
    28621 ccgataacct gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct
    28681 gcgctggcgc taaatcccgg aatcgccccc tgccagtcaa gattcagctt cagccgctcc
    28741 gggcaataaa taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg
    28801 tcagcatgaa tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca
    28861 tgcagaccat taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca
    28921 aagacatctt gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa
    28981 tcatctttga gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt
    29041 tgattattcg caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaaacc
    29101 atcccgtccc tgacgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga
    29161 aaagtggtta ctgtcgctga atccacagcg ataggcgatg tcagtaacgc tggcctcact
    29221 gtggcgtagc agatgtcggg ccttcatcag tcgcaggcgg ttcaggtaac gctgaggcgt
    29281 cagtcccgtt tgctgcttaa gctgccgatg tagcgttcgc agtgaaagag aaaattgatc
    29341 cgccacggca tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa
    29401 gttgagacgt gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag
    29461 cagtaattgc ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc
    29521 ctgctgttcc atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg
    29581 ccagtgagac ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag
    29641 aaactgaaat cgatccggcg agcgatatag cacattggtc agacacagat tatcggtatg
    29701 ttcatacaga tgccgatcat ggtcgcgcac aaaacagacc gtgccaccgg tgatggtata
    29761 gggctgccca ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa
    29821 atcatgatga tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc
    29881 gttaccagac ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg
    29941 tcaacacggc gaaatagtaa tcacgaggtc aggttcttac cttaaatttt caacgggaaa
    30001 ccacgtaaaa aacgtcgatt tttcaagata cagcatgaat tttcaggaaa tgcggtgagc
    30061 atcacaccac cacaattcag caatttgtga acatcatcac gttcatcttt ccctggttgc
    30121 caatggccca ttttcctgtc agtagcgaga aggtcgcgaa ttcaggcgct ttttagactg
    30181 gtcgtaatga aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc
    30241 tcggcgcatc cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga
    30301 cgctgcgcga aatccatcgt tttaaaaatg ggctgcatag ccagaacggt tatgtcacct
    30361 gggatgtgga tagcctggaa agtgccattc gccttggatt aaacatggtg tgtgaggaag
    30421 ggattcgtat cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc
    30481 aacagggtca gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctaa
    30541 tggcgcaggc acagcaacaa ctcggcaaac gcgatattta tcaacgtagc ggcatccagt
    30601 ttctgccctt caatacgctt tatcagttgc gtgcgctgac ggagcaacaa cctgaactta
    30661 ttccacacat tgctcacgct ctgctgatgc cggattactt cagttatcgc ctgaccggca
    30721 agatgaactg ggagtacacc aatgccacca ccacacaact ggtcaatatc aatagcgacg
    30781 actgggacga gtcgctactg gcgtggagcg gggccaacaa agcctggttt ggtcgcccga
    30841 cgcatccggg taatgtcatt gggcactgga tttgcccgca gggtaatgag attccggtgg
    30901 tcgccgttgc cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac
    30961 gcgccgctta tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgctat
    31021 ttaccaatga cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct
    31081 atcgggtgct gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggaacggc
    31141 aaatcaacga tctcccggcg cttatcgccg cgacacaggc acttccggcc tgccgcttca
    31201 tcatcaatcc caatgacgat cgctttatta atcctgaggc gatgtgcagc gaaattcagg
    31261 ctgcgtgtcg ggaaacggcg caaccgatcc cggaaagtga tgctgaactg gcgcgctgta
    31321 ttttcgacag tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg
    31381 gtgaagattt ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc
    31441 agctatgcgc cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcgacgc
    31501 tcggcaatat cggcatccag ttaatgacgc tggatgaact caacaatgtg gatgatttcc
    31561 gtcaggtcgt cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa
    31621 ttgcccacta tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac
    31681 cactcaactg gaacaggcct gggagctggc gaaacagcgt ttcgcggcag tagggattga
    31741 tgtcgaggag gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg
    31801 cgatgatgtt tccggttttg aaaacccgga agattcgctg accggtggga ttcaggctac
    31861 tggtaattat ccgggcaaag cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc
    31921 tatgcggctg attccggggc caaaacggct taatttacat gccatctatc tggaatcaga
    31981 cacgccagtc tcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc
    32041 gaaagccaat cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc
    32101 cgatggcttt acgctttccc atgccgacga cagcattcgc cagttctgga ttgatcactg
    32161 caaagccagc cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat
    32221 gaacatctgg atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca
    32281 gcgtctgctg gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat
    32341 cgacgccgtt gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa
    32401 tgagttttac atggggtatg cctccagccg ccagactgcg ctgtgcctgg acgccgggca
    32461 cttccacccg actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtgccgca
    32521 gttgctgctg cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga
    32581 tgatgaaacc caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca
    32641 tatcggcctt gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac
    32701 acgcaatatg aaaaaagccc tgctgcgtgc gttgctggaa cctaccgccg agctgcgcaa
    32761 gttggaagcg gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt
    32821 gccgtggcag gcggtctggg aaatgtattg ccaacgtcac gataccccaa cgggtagcga
    32881 atggctggag agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact
    32941 gccggatgcg gcacgagcgc cttatccggc ctacgggtcg aatgtccatg cgggctgtca
    33001 ccctcacccc ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa
    33061 cggagctgaa cactgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgag
    33121 gggaacaagc agcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc
    33181 aaaacattac ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctggc
    33241 tgaaaggctg ggacgagcgc aacggcggta acctgacgct gcgcctggat gacgccgata
    33301 tcgcgcctta taaagacaac ttccatgcac aaccgcgtta tatcccgctc agccaaccca
    33361 tgcctttact ggcaaataca ccgtttattg tcaccggctc cggcaaattc ttccgtaacg
    33421 tccagcttga tcctgcagct aacttaggcg tcgtaaaagt cgacagcgac ggcgcgggct
    33481 accatattct ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact
    33541 ttctctccca ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact
    33601 gccacgccac caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta
    33661 ctcgccaact gtgggaaggc agcaccgagt gtctggtggt attcccggat ggcgttggca
    33721 ttctgccgtg gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc
    33781 aaaaacattc gctggtgttg tggcccttcc acggcgtctt cggcagcggg tcgacgttgg
    33841 atgaaacatt cggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt
    33901 attcgatggg cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc
    33961 gcttcggcgt tacaccactc gccagtgcgc tggcgctgta aggagcaaac atgatccgca
    34021 aagcctttgt catgcaggtt aaccccgacg cccacgaaga gtatcagcgt cggcataacc
    34081 ccatctggcc ggaactggaa gcagtgttga aatctcacgg tgtgcataac tacgccattt
    34141 acctcgacaa agcgcgtaat ctgctgtttg ccatggtaga gattgaatct gaagaacggt
    34201 ggaatgcggt agccagtact gatgtttgcc aacgttggtg gaaatatatg accgatgtta
    34261 tgcccgctaa cccggataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc
    34321 cgtaattgcc caatgtgccg gagatgcctc cggcacaata ttttaaattt cgatttgaaa
    34381 aattaaacat attacatctc ccggtaagtt atatttccct gatacattgt gagtaaatca
    34441 caaaaataat gaataaccca ttaatgattc atgtggttta tttaaataac cattatgtgc
    34501 attactccgc aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc
    34561 gcctctcatc acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc
    34621 gattaatggc agttcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt
    34681 attccgttct gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac
    34741 gccagttcct gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg
    34801 gtgtcgccgt gccgcacggt aaaagcgcct gcgttaaaca accgttcgta ttattcgccc
    34861 gcaaagcgca ggctattgac tggaaagcca gcgatggtga agacgtcaat tgctggatct
    34921 gcctcggcgt gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc
    34981 gcaaaattat tcacaaggaa tttattcatc aacagcaaca gggcgatacc gaccaggtgc
    35041 ttgccttgtt aaatcaaacc ctcagctcat aaggaagtgg cgatggattc atccttacgt
    35101 attgtcgcga tcaccaactg ccccgccggg atcgctcaca cctacatggc ggcggaagcc
    35161 ctggaacaga aagcccgttc tctcggtcat accataaaag tggaaactca agggtccagt
    35221 ggcgttgaaa accgcttatc cagcgaagag attgccgctg ccgattacgt cattctcgct
    35281 accgggcgtg gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag
    35341 attgccatct cccaggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac
    35401 tcgcagcttt ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc
    35461 agcgtgatga gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt
    35521 ggtatcctgg tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg
    35581 tcgaaaggcg cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc
    35641 tttatgatcc ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc
    35701 gctccggcgt ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg
    35761 ggtgcgggat tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg
    35821 ttccgcaaag tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg
    35881 ttcgttactc tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc
    35941 gacctcatgg gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg
    36001 gcagcgtttc tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc
    36061 gcctggttct tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc
    36121 ggcgttgtcg cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa
    36181 ctgtttaccc gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc
    36241 gcaaccgaac cggctatacc ttacgctctg gcagctccgt tgccgatgat taccgccaat
    36301 acgctggcgg gtggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca
    36361 cctggtttgg gtatctttga cccgctgatc ggcctgatgt cgccggtggg ttcgttttat
    36421 ctggtgctgg cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggattg
    36481 tggttacgcc gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact
    36541 gcaacagttg tgcgaagcca gcgccgtcag cggcgatgaa caggaagttc gcgacattct
    36601 gataaacacg ctggaacctt gcgttaatga gatcaccttt gatggtctgg gcagctttgt
    36661 tgcccgtaag gggaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg
    36721 ctttatggtc acccacatcg acgagagcgg ttttctgcgt ttcaccacca ttggtggctg
    36781 gtggaatcag tcgatgctca accaccgggt aaccatacgc acacacaagg gagtgaaaat
    36841 ccctggtgtg attggttccg tcgcgcctca tgcgttaacg gaaaagcaaa ggcaacagcc
    36901 gctgtcattt gatgagatgt tcattgatat tggcgcaaac agtcgcgatg aggtggaaaa
    36961 acgcggtgtg gcgatgggcg attttattag cccggaagcc aattttgcct gctggggcga
    37021 agataaagtg gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga tggctgaact
    37081 attgcagacg gtgaataatc ccgaaattac gctgtatggc gtcggcagcg tggaagaaga
    37141 agttgggcta cgcggggcac aaacctcggc ggaacacatt aaaccggatg tagtgatcgt
    37201 gctggatacc gctgtcgcag gcgatgttcc gggcattgat aacattaaat acccgctgaa
    37261 actgggccag gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact
    37321 ggtagcagcg ttaaaaagct gtgccgcaca taacgattta ccgctgcaat tttccaccat
    37381 gaaaaccggt gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt
    37441 tgcgctgtgc ctgccaaccc gctatctgca cgctaacagc ggtatgattt caaaagccga
    37501 ttacgatgct ctgctcacgt tgatacggga ttttctgacc accttaaccg cggagaaagt
    37561 caacgcgttt agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt
    37621 gtcgatctgc tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt
    37681 tcaggcagga ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc
    37741 cgcatttccg ccagtggtag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt
    37801 tttcagttgc tgcaaaagca cgataacgac gatcggctgc tggcactgtt attactgaat
    37861 acctttactc cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtcgca
    37921 gagcgtctgc cccggttaaa acagcgttat gaacgcgctt gttgcctggc cagccgcccc
    37981 ggtttaggcc atttcattga tgagacagaa gataagcgcg ttatcttgct ggcgaacttg
    38041 ctgcgcaaag atccgttttt aattccgctg gcgggcgtaa cacgagacaa ccttcagcat
    38101 ttatccacgg cctgcgacaa ccaacaccgc tggccgctca tgcagggtga ttatctctcc
    38161 agcctgattc tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaatat
    38221 cccggtgacg agataaaaca aatcgttgaa caaagcggca tgtttcttgg tgataacgct
    38281 gtaagaaccc tgacaggttt gatagagaaa cagcatcagc aagcgcagat aatttctgcc
    38341 gataatgtgc agcggttgct gcaaagggtg ccgggcatcg cgtcattgaa tattattgat
    38401 acgcggctgg ttgagaacat taccgggcat ttattacgtt gccttgccgc accagtgtgg
    38461 attgctgagc accgccaaag caccatgaat aacctgaaag cagcctggcc tgcggccttt
    38521 gatatgagtc tgcactttat tacgctactg cgtgaacagc tcgatattcc ccttttcgac
    38581 agcgatctga tcggtttgta ttttgcctgt gcgctggaac ggcatcaaaa cgaacgccag
    38641 ccgatcattt tgctctcgga ccagaacgcg attgccacta ttaatcagct tgccattgag
    38701 cgcgatgttt tacattgtcg ggtgattatt gcccgtagct taagcgaact tgttgccatt
    38761 cgcgaagaga ttgagccgtt attgatcatt aacaacagcc attatttact ggatgacgcg
    38821 gtaaataatt acatcaccgt aaaaaatatc attacggctg ccggtatcga acaaataaaa
    38881 cattttctgg cgacggcatt tattcgccaa cagccggagc gttttttctc tgcccccgga
    38941 agttttcatt attcgaatgt acgcggtgaa agctggcaac atattacccg gcaaatttgt
    39001 gcgcaattag tcgcacaaca ccatattacc gccgatgaag cacaacgcat catcgcccgc
    39061 gaaggcgaag gtgaaaacct gattgttaat cgcctcgcca tcccacattg ctggagcgaa
    39121 caggagcgac gttttcgtgg attttttatt accctcgccc aaccagttga ggtgaataac
    39181 gaagtcctta accatgtctt gatcgcctgc gccgccgccg atgcgcgtca tgagctaaaa
    39241 atatttagct atctggcaag catattgtgt cagcatccgg cggagattat tgccgggtta
    39301 acaggatatg aggcatttat ggagttactt cacaaggggt gaactgtgtc ggatgcgccg
    39361 catccgacac tgcatttatc atcaaaaacg tttaacgcag gctattatag tcatcaacca
    39421 tttcgttgta ctcgcgtaat gctctcggga aagagtcttc gaccatccag cttgagtttg
    39481 cgtcctgaag ttgctcttta tcccagtcag agtacgggac tttatctctg atgcggcgaa
    39541 cgtatttttt agcctcgagt tgatactggc ctgccgaatt aataaacgag aaattcatgc
    39601 cgcctttatc cgcttctttg gcctgcgcca ctagcgtttc cagttcagac actttcttca
    39661 ttgccgcatc aacatcaaac ttctcttgcg atatcaggtt attaatttgt ttcgctgaaa
    39721 tcatgactgc cagagagtag tagtggaatg ttttcccttc tctttcttca atatttttca
    39781 gttccgcaag ctggcgctta tcgttaattt cctgaatcgc cgcatgataa gattctgcta
    39841 caggttcaaa ggtttccaga ttttttaaga atgtctggtg cagcgttttc cctttcgcaa
    39901 acgcatcgtc tttataattt tcctgggtgt aatatttatc catttcgtta atggtattac
    39961 ccaacgccac ggcagcatcg atataactca ccgccacgcc atcaattggt tgcagcgcag
    40021 gcgttaacgc cacagcactt tttacgcctt tttcacactc agcgaggttg gattcactaa
    40081 tgccgtagat gccataaaca gtacgctctt caccggtagg cccctgttta aaatctttca
    40141 gccagactgc tctgtacagg gatctgtaac ttgttatagc acttgatata gacatccatt
    40201 ttttcgtcga aaaccgcttg ttcatcgacc accggttgcg caggtgtttc aggtttagct
    40261 tctgcttttg caacgggagc ttcaggagct ggtgcggcag gttgactatt ggcaggcggg
    40321 ggcgtttctg tttcggcttt tttatcatca caacccgtca gaccgagggc catgatggcg
    40381 actataatag cggaagataa taaattcctt ttcatttaat tgcctt
//
LOCUS       DAZKHC020000035        40395 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZKHC020000035 DAZKHC020000000
VERSION     DAZKHC020000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 40395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.035"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            235..321
                     /gene="leuL"
                     /locus_tag="ACHOSM_RS10690"
                     /old_locus_tag="pgaptmp_002138"
     CDS             235..321
                     /gene="leuL"
                     /locus_tag="ACHOSM_RS10690"
                     /old_locus_tag="pgaptmp_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HGU4558654.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            414..1985
                     /gene="leuA"
                     /locus_tag="ACHOSM_RS10695"
                     /old_locus_tag="pgaptmp_002139"
     CDS             414..1985
                     /gene="leuA"
                     /locus_tag="ACHOSM_RS10695"
                     /old_locus_tag="pgaptmp_002139"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HGU4558655.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINNQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            1985..3076
                     /gene="leuB"
                     /locus_tag="ACHOSM_RS10700"
                     /old_locus_tag="pgaptmp_002140"
     CDS             1985..3076
                     /gene="leuB"
                     /locus_tag="ACHOSM_RS10700"
                     /old_locus_tag="pgaptmp_002140"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGU4558656.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3079..4479
                     /gene="leuC"
                     /locus_tag="ACHOSM_RS10705"
                     /old_locus_tag="pgaptmp_002141"
     CDS             3079..4479
                     /gene="leuC"
                     /locus_tag="ACHOSM_RS10705"
                     /old_locus_tag="pgaptmp_002141"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HGU4558657.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4490..5095
                     /gene="leuD"
                     /locus_tag="ACHOSM_RS10710"
                     /old_locus_tag="pgaptmp_002142"
     CDS             4490..5095
                     /gene="leuD"
                     /locus_tag="ACHOSM_RS10710"
                     /old_locus_tag="pgaptmp_002142"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGU4558658.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5144..6322)
                     /gene="setA"
                     /locus_tag="ACHOSM_RS10715"
                     /old_locus_tag="pgaptmp_002143"
     CDS             complement(5144..6322)
                     /gene="setA"
                     /locus_tag="ACHOSM_RS10715"
                     /old_locus_tag="pgaptmp_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HGU4558659.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKCSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6424..6555)
                     /gene="sgrT"
                     /locus_tag="ACHOSM_RS10720"
                     /old_locus_tag="pgaptmp_002144"
     CDS             complement(6424..6555)
                     /gene="sgrT"
                     /locus_tag="ACHOSM_RS10720"
                     /old_locus_tag="pgaptmp_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HGU4558660.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6644..8299
                     /gene="sgrR"
                     /locus_tag="ACHOSM_RS10725"
                     /old_locus_tag="pgaptmp_002145"
     CDS             6644..8299
                     /gene="sgrR"
                     /locus_tag="ACHOSM_RS10725"
                     /old_locus_tag="pgaptmp_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HGU4558661.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANKQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8333..8432
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8463..9446
                     /gene="thiB"
                     /locus_tag="ACHOSM_RS10730"
                     /old_locus_tag="pgaptmp_002146"
     CDS             8463..9446
                     /gene="thiB"
                     /locus_tag="ACHOSM_RS10730"
                     /old_locus_tag="pgaptmp_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HGU4558662.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9422..11032
                     /gene="thiP"
                     /locus_tag="ACHOSM_RS10735"
                     /old_locus_tag="pgaptmp_002147"
     CDS             9422..11032
                     /gene="thiP"
                     /locus_tag="ACHOSM_RS10735"
                     /old_locus_tag="pgaptmp_002147"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HGU4558663.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVEQRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11016..11714
                     /gene="thiQ"
                     /locus_tag="ACHOSM_RS10740"
                     /old_locus_tag="pgaptmp_002148"
     CDS             11016..11714
                     /gene="thiQ"
                     /locus_tag="ACHOSM_RS10740"
                     /old_locus_tag="pgaptmp_002148"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HGU4558664.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQHKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(11784..12548)
                     /gene="yabI"
                     /locus_tag="ACHOSM_RS10745"
                     /old_locus_tag="pgaptmp_002149"
     CDS             complement(11784..12548)
                     /gene="yabI"
                     /locus_tag="ACHOSM_RS10745"
                     /old_locus_tag="pgaptmp_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4558665.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12634..13512)
                     /gene="araC"
                     /locus_tag="ACHOSM_RS10750"
                     /old_locus_tag="pgaptmp_002150"
     CDS             complement(12634..13512)
                     /gene="araC"
                     /locus_tag="ACHOSM_RS10750"
                     /old_locus_tag="pgaptmp_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HGU4558666.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGGVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13851..15551
                     /gene="araB"
                     /locus_tag="ACHOSM_RS10755"
                     /old_locus_tag="pgaptmp_002151"
     CDS             13851..15551
                     /gene="araB"
                     /locus_tag="ACHOSM_RS10755"
                     /old_locus_tag="pgaptmp_002151"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HGU4558667.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15562..17064
                     /gene="araA"
                     /locus_tag="ACHOSM_RS10760"
                     /old_locus_tag="pgaptmp_002152"
     CDS             15562..17064
                     /gene="araA"
                     /locus_tag="ACHOSM_RS10760"
                     /old_locus_tag="pgaptmp_002152"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HGU4558668.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHNIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17264..17959
                     /gene="araD"
                     /locus_tag="ACHOSM_RS10765"
                     /old_locus_tag="pgaptmp_002153"
     CDS             17264..17959
                     /gene="araD"
                     /locus_tag="ACHOSM_RS10765"
                     /old_locus_tag="pgaptmp_002153"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGU4558669.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCHQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18034..20385
                     /gene="polB"
                     /locus_tag="ACHOSM_RS10770"
                     /old_locus_tag="pgaptmp_002154"
     CDS             18034..20385
                     /gene="polB"
                     /locus_tag="ACHOSM_RS10770"
                     /old_locus_tag="pgaptmp_002154"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HGU4558670.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRLPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20549..23455
                     /gene="rapA"
                     /locus_tag="ACHOSM_RS10775"
                     /old_locus_tag="pgaptmp_002155"
     CDS             20549..23455
                     /gene="rapA"
                     /locus_tag="ACHOSM_RS10775"
                     /old_locus_tag="pgaptmp_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HGU4558671.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23467..24126
                     /gene="rluA"
                     /locus_tag="ACHOSM_RS10780"
                     /old_locus_tag="pgaptmp_002156"
     CDS             23467..24126
                     /gene="rluA"
                     /locus_tag="ACHOSM_RS10780"
                     /old_locus_tag="pgaptmp_002156"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HGU4558672.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPHLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24243..25058)
                     /gene="djlA"
                     /locus_tag="ACHOSM_RS10785"
                     /old_locus_tag="pgaptmp_002157"
     CDS             complement(24243..25058)
                     /gene="djlA"
                     /locus_tag="ACHOSM_RS10785"
                     /old_locus_tag="pgaptmp_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HGU4558673.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLLMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25313..27667
                     /gene="lptD"
                     /locus_tag="ACHOSM_RS10790"
                     /old_locus_tag="pgaptmp_002158"
     CDS             25313..27667
                     /gene="lptD"
                     /locus_tag="ACHOSM_RS10790"
                     /old_locus_tag="pgaptmp_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HGU4558674.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27720..29006
                     /gene="surA"
                     /locus_tag="ACHOSM_RS10795"
                     /old_locus_tag="pgaptmp_002159"
     CDS             27720..29006
                     /gene="surA"
                     /locus_tag="ACHOSM_RS10795"
                     /old_locus_tag="pgaptmp_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HGU4558675.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29006..29995
                     /gene="pdxA"
                     /locus_tag="ACHOSM_RS10800"
                     /old_locus_tag="pgaptmp_002160"
     CDS             29006..29995
                     /gene="pdxA"
                     /locus_tag="ACHOSM_RS10800"
                     /old_locus_tag="pgaptmp_002160"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HGU4558676.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSIITALNLAIKMIVNTQ"
     gene            29992..30813
                     /gene="rsmA"
                     /locus_tag="ACHOSM_RS10805"
                     /old_locus_tag="pgaptmp_002161"
     CDS             29992..30813
                     /gene="rsmA"
                     /locus_tag="ACHOSM_RS10805"
                     /old_locus_tag="pgaptmp_002161"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGU4558677.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30816..31193
                     /gene="apaG"
                     /locus_tag="ACHOSM_RS10810"
                     /old_locus_tag="pgaptmp_002162"
     CDS             30816..31193
                     /gene="apaG"
                     /locus_tag="ACHOSM_RS10810"
                     /old_locus_tag="pgaptmp_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HGU4558678.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31200..32042
                     /gene="apaH"
                     /locus_tag="ACHOSM_RS10815"
                     /old_locus_tag="pgaptmp_002163"
     CDS             31200..32042
                     /gene="apaH"
                     /locus_tag="ACHOSM_RS10815"
                     /old_locus_tag="pgaptmp_002163"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HGU4558679.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32120..32599)
                     /gene="folA"
                     /locus_tag="ACHOSM_RS10820"
                     /old_locus_tag="pgaptmp_002164"
     CDS             complement(32120..32599)
                     /gene="folA"
                     /locus_tag="ACHOSM_RS10820"
                     /old_locus_tag="pgaptmp_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HGU4558680.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32790..34652)
                     /gene="kefC"
                     /locus_tag="ACHOSM_RS10825"
                     /old_locus_tag="pgaptmp_002165"
     CDS             complement(32790..34652)
                     /gene="kefC"
                     /locus_tag="ACHOSM_RS10825"
                     /old_locus_tag="pgaptmp_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HGU4558681.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFAVSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34645..35175)
                     /gene="kefF"
                     /locus_tag="ACHOSM_RS10830"
                     /old_locus_tag="pgaptmp_002166"
     CDS             complement(34645..35175)
                     /gene="kefF"
                     /locus_tag="ACHOSM_RS10830"
                     /old_locus_tag="pgaptmp_002166"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HGU4558682.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35283..36614)
                     /gene="yaaU"
                     /locus_tag="ACHOSM_RS10835"
                     /old_locus_tag="pgaptmp_002167"
     CDS             complement(35283..36614)
                     /gene="yaaU"
                     /locus_tag="ACHOSM_RS10835"
                     /old_locus_tag="pgaptmp_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4558683.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EAVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGSGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(36673..36960)
                     /gene="fixX"
                     /locus_tag="ACHOSM_RS10840"
                     /old_locus_tag="pgaptmp_002168"
     CDS             complement(36673..36960)
                     /gene="fixX"
                     /locus_tag="ACHOSM_RS10840"
                     /old_locus_tag="pgaptmp_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HGU4558684.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(36957..38243)
                     /gene="fixC"
                     /locus_tag="ACHOSM_RS10845"
                     /old_locus_tag="pgaptmp_002169"
     CDS             complement(36957..38243)
                     /gene="fixC"
                     /locus_tag="ACHOSM_RS10845"
                     /old_locus_tag="pgaptmp_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4558685.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38294..39235)
                     /gene="fixB"
                     /locus_tag="ACHOSM_RS10850"
                     /old_locus_tag="pgaptmp_002170"
     CDS             complement(38294..39235)
                     /gene="fixB"
                     /locus_tag="ACHOSM_RS10850"
                     /old_locus_tag="pgaptmp_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGU4558686.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39250..40020)
                     /gene="fixA"
                     /locus_tag="ACHOSM_RS10855"
                     /old_locus_tag="pgaptmp_002171"
     CDS             complement(39250..40020)
                     /gene="fixA"
                     /locus_tag="ACHOSM_RS10855"
                     /old_locus_tag="pgaptmp_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HGU4558687.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9734 a        11139 c        10614 g         8908 t
ORIGIN      
        1 cctttttcct cgttgattag atgcaaaaat ttattctgaa atatgtcaac ctatgaaaag
       61 tgtcggtcgt taaacagaaa ttaatatcgc ttgctttaac caccgcagca caattagcta
      121 attttacgga tgcagaactc gcgctggcgg gacgttttta ttgcgtcagg gttgacatcc
      181 gtttttgtat ccagtaactc taaaagcata tcgcattcat ctggagctga tttaatgact
      241 cacatcgttc gctttatcgg tctactacta ctaaacgcat cttctttgcg cggtagacga
      301 gtgagcggca tccagcatta agccagcacg cagtcaaaca aaaaacccgc gccattgcgc
      361 gggttttttt atgcccgaag cgaggcgctc taaaagagac aaggacccaa accatgagcc
      421 agcaagtcat tattttcgat accacattgc gcgacggtga acaggcgtta caggcaagct
      481 tgagtgtgaa agaaaaactg caaattgcgc tggcccttga gcgtatgggt gttgacgtga
      541 tggaagtcgg tttccccgtc tcttcgccgg gcgattttga atcagtgcaa accatcgccc
      601 gccaggttaa aaacagccgc gtatgcgcgt tagctcgctg cgtggagaaa gatatcgacg
      661 tggcggccga atccctgaaa gtcgccgaag ccttccgtat tcataccttt attgccactt
      721 cgccaatgca catcgccacc aagctgcgca gcacgctgga tgaagtgatc gaacgcgcta
      781 tctatatggt gaaacgcgcc cgtaattaca ccgatgatgt tgaattttct tgcgaagatg
      841 ccgggcgtac ccccattgcc gatctggcgc gagtggtcga agcggcgatt aatgccggtg
      901 ccaccaccat caacattccg gacaccgtgg gctacaccat gccgtttgag ttcgccggaa
      961 tcatcagcgg tctgtatgaa cgcgtaccta acatcgacaa agccattatt tccgtacata
     1021 cccacgacga tttaggcctg gcggtcggca actcactggc ggcggtacat gccggtgcac
     1081 gccaggtgga aggtgcaatg aacgggatcg gcgagcgtgc cggtaactgt tcgctggaag
     1141 aagtcatcat ggcgattaaa gttcgtaagg atattctcaa cgtccacacc gccattaata
     1201 accaggagat atggcgcacc agccagttag ttagccagat ttgtaatatg ccgatcccgg
     1261 caaacaaagc cattgttggc agcggcgcat tcgctcactc ctccggtatc caccaggatg
     1321 gcgtgctgaa aaaccgcgaa aactacgaaa tcatgacacc agaatctatt ggtctgaacc
     1381 aaatccagct gaatctgacc tctcgttcgg ggcgtgcggc ggtgaaacat cgcatggatg
     1441 agatggggta taaagaaagt gaatataatt tagacaattt gtacgatgct ttcctgaagc
     1501 tggcggacaa aaaaggtcag gtgtttgatt acgatctgga ggcgctggcc ttcatcggta
     1561 agcagcaaga agagccggag catttccgtc tggattactt cagcgtgcag tctggctcta
     1621 acgatatcgc caccgccgcc gtcaaactgg cctgtggcga agaagtcaaa gcagaagccg
     1681 ccaacggtaa cggtccggtc gatgccgtct atcaggcaat taaccgcatc actgactata
     1741 acgtcgaact ggtgaaatac agcctgaccg ccaaaggtca cggtaaagat gcgctgggtc
     1801 aggtggatat tgtcgccaac tacaacggtc gccgcttcca cggcgtcggc ctggccaccg
     1861 atattgtcga gtcctccgcc aaagccatgg tgcacgtact gaacaatatc tggcgtgccg
     1921 cagaagtcga aaaagagttg caacgcaaag ctcaacacaa cgaaaacaac aaggaaaccg
     1981 tgtgatgtcg aagaattacc atattgccgt attgccgggg gacggtattg gtccggaagt
     2041 gatgacccag gcgctgaaag tgctggatgc cgtgcgcaac cgctttgcga tgcgcatcac
     2101 caccagccat tacgatgtag gcggcgcagc cattgataac cacgggcaac cactgccgcc
     2161 tgcgacggtt gaaggttgtg agcaagccga tgccgtgctg tttggctcgg taggcggtcc
     2221 gaaatgggaa catttaccac cagaccagca accagaacgc ggcgcgctgt tgcctttgcg
     2281 taagcacttc aaattattca gcaacctgcg tccggcaaaa ctgtatcagg ggctggaagc
     2341 attctgtccg ctgcgtgctg acattgccgc taacggcttc gacatcctgt gcgtgcgcga
     2401 actgaccggc ggcatctatt tcggtcagcc aaaaggccgc gaaggtagcg gacagtatga
     2461 aaaagcgttt gataccgagg tgtatcaccg ttttgagatc gaacgtatcg cccgcatcgc
     2521 gtttgaatct gcccgcaagc gtcgccacaa agtcacctca atcgacaaag ccaacgtgct
     2581 gcaatcctct attttatggc gggagatcgt taacgagatc gccacggaat acccggatgt
     2641 cgaactggcg catatgtaca tcgacaacgc caccatgcag ctgattaaag atccatcaca
     2701 gtttgacgtc ctgctgtgct ccaacctgtt tggcgacatt ctgtctgacg agtgcgcaat
     2761 gatcactggc tcgatgggga tgttgccttc cgccagcctg aacgagcaag gttttggtct
     2821 gtatgaaccg gcaggcggct cagcaccaga tatcgcaggc aaaaacatcg ccaacccgat
     2881 tgcgcaaatt ctgtcgctgg cactgctgct gcgctacagc ctggatgccg atgatgcggc
     2941 ttgcgccatt gaacgcgcca ttaaccgcgc attagaagaa ggcattcgca ccggggattt
     3001 agcccgtggc gctgccgccg ttagtaccga tgaaatgggc gatatcattg cccgctatgt
     3061 ggcagaaggg gtgtaatcat ggctaaaacg ttatacgaaa aattattcga cgctcacgta
     3121 gtgtacgaag ccgaaaacga aaccccgctg ttatatatcg accgccacct ggtgcatgaa
     3181 gtgacctcac cgcaggcgtt cgatggtctg cgcgcccacg gtcgcccggt acgtcagccg
     3241 ggcaaaacct tcgccactat ggatcacaac gtctctaccc agacgaaaga cattaatgcc
     3301 tgtggtgaaa tggcgcgcat ccagatgcag gagctgatta aaaactgcaa agaatttggc
     3361 gtcgaactgt atgacctgaa tcacccgtat caggggatcg tccacgtaat ggggccggaa
     3421 cagggcgtca ccttgccggg gatgaccatt gtctgcggcg actcgcatac cgccacccac
     3481 ggcgcgtttg gcgcactggc ctttggtatc ggcacttccg aagttgaaca cgtactggca
     3541 acgcaaaccc tgaaacaggg ccgcgcaaaa accatgaaaa ttgaagtcca gggcaaagcc
     3601 gcgccgggca ttaccgcaaa agatatcgtg ctggcaatta tcggtaaaac cggtagcgca
     3661 ggcggcaccg ggcatgtggt ggagttttgc ggcgaagcaa tccgtgattt aagcatggaa
     3721 ggtcgtatga ccctgtgcaa tatggcaatc gaaatgggcg caaaagccgg tctggttgca
     3781 ccggacgaaa ccacctttaa ctatgtcaaa ggccgtttgc atgcgccgaa aggcaaagat
     3841 ttcgacgacg ccgttgccta ctggaaaacc ctgcaaaccg acgaaggcgc aactttcgat
     3901 accgttgtca ctctgcaagc agaagaaatt tcaccgcagg tcacctgggg caccaatcca
     3961 ggccaggtga tttccgtgaa cgacaatatt cccgatccgg cttcgtttgc cgatccggtt
     4021 gaacgcgcgt cggcagaaaa agcgctggcc tatatggggc tgaaaccggg cattccgctg
     4081 accgaagtgg ctatcgacaa agtgtttatc ggttcctgta ccaactcgcg cattgaagat
     4141 ttacgcgcgg cagcggagat cgccaaaggg cgaaaagtcg cgccaggcgt gcaggcactg
     4201 gtggttcccg gctctggccc ggtaaaagcc caggcggaag cggaaggtct ggataaaatc
     4261 tttattgaag ccggttttga atggcgcttg cctggctgct caatgtgtct ggcgatgaac
     4321 aacgaccgtc tgaatccggg cgaacgttgt gcatccacca gcaaccgtaa ctttgaaggc
     4381 cgccaggggc gcggcgggcg cacgcatctg gtcagcccgg caatggctgc cgctgctgct
     4441 gtgaccggac atttcgccga cattcgcaac attaaataag gagcacatca tggcagagaa
     4501 atttatcaaa cacacaggcc tggtggttcc gctggatgcc gccaatgtcg ataccgatgc
     4561 aatcatcccg aaacagtttt tgcagaaagt gacccgtacg ggttttggcg cgcatctgtt
     4621 taacgactgg cgttttctcg atgaaaaagg ccaacagcca aacccggact tcgtgctgaa
     4681 cttcccgcag tatcagggcg cttccatttt gctggcacga gaaaacttcg gctgtggctc
     4741 ctcgcgtgag cacgcgccct gggcattgac cgactacggt tttaaagtgg tgattgcgcc
     4801 gagttttgct gacatcttct acggcaatag ctttaacaac cagctgctgc cggtgaaatt
     4861 aagcgatgca gaagtggacg aactgtttgc gctggtgaaa gccaatccgg ggatccattt
     4921 cgacgtggat ctggaagcgc aagaggtgaa agcgggagag aaaacctatc gctttaccat
     4981 cgatgccttc cgccgccact gcatgatgaa cggtctggac agtattgggc ttaccttgca
     5041 gcacgacgac gccattgccg cttatgaagc aaaacaacct gcgtttatga attaatcccc
     5101 ttgcccggtc aaatgaccgg gctttccgct atcgtccacg tcatcaaacg tctttaacct
     5161 ttgcggttaa aaataatgcg acaacagaaa taaccgcaat tacccagtag acagcaaagt
     5221 gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc ccggtagaaa
     5281 tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa tcctgaaacc
     5341 atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt tgcagcgtca
     5401 tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact cctgccgcca
     5461 ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca gccagaatca
     5521 ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt ttgtctggca
     5581 atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg ttgcaggtcc
     5641 acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag ccaccttgca
     5701 ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc ataaatgcaa
     5761 tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc gtaaagccgt
     5821 aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga gaaagctgcg
     5881 cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca tattcccgcg
     5941 ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc acaccacagg
     6001 tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg atagccatca
     6061 aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacat tttgccaacc
     6121 agaggcttac gccgatccct gcaatagcat tcaccgtata aaagaggccg atccagaaag
     6181 gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt agcgccccgg
     6241 ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc atacggcgag
     6301 ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata aaaaaaacca
     6361 gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca tgatgcaggc
     6421 aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca tggttaatcg
     6481 ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa aatagtgctg
     6541 ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg acttaatata
     6601 gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat ctgctcgtct
     6661 gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg acacaacgct
     6721 caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc tgctcaacac
     6781 catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta aacgctcgcg
     6841 tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag acctgctgga
     6901 gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg tgcggcaaat
     6961 gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc gcgtgctcta
     7021 ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg aaacccatat
     7081 tgcccggcaa atcttcagtt cgctaacgcg cataaatgag gaaaatgggg aactggaagc
     7141 agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct ttttgcgtcc
     7201 aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg cctctttaaa
     7261 acgaatcaat acgctgccgc tctattcgca tattgctgac attgtgtcgc cgacgccctg
     7321 gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttattgc tggggcaagt
     7381 tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca gccatcccat
     7441 cggcaccggt ccgtatgcgg tgatccgcaa cagcaccaat caactgaaaa ttcaggcatt
     7501 cgatgacttc ttcggttacc gggcattaat cgacgaagtt aacgtctggg ttctgccgga
     7561 aattgccgac gagccagccg gaggtctgat gctaaaaggt ccacagggcg aggaaaaaga
     7621 gattgaaagc cgcctggagg aaggttgcta ctatttactg ttcgatagcc gcacccatcg
     7681 cggggcgaat aagcaagtca gggactgggt aagctatgtg ctttctccaa ctaatctggt
     7741 ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac tgctcccccg
     7801 ttggcaccat gcccgcacca taaagagcga aaaaccggct ggtctggaaa gcctcaccct
     7861 gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc agcagattct
     7921 ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt ggcatgaagg
     7981 agagatcgag agcgatatct ggcttaatag cgccaacttt acgctgccgc tggatttttc
     8041 gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga tcgactggca
     8101 agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt gccagcaact
     8161 ggtcgccagt aaagcaatgg tgccgcttat ccaccactgg ctgatcattc agggacaacg
     8221 cagtatgcgc ggcctgcgta tgaataccct cggctggttc gattttaaat cagcgtggtt
     8281 tgcgccgccg gatccatgat tgctgctaga taacaaaatc actacactaa cgccgttctc
     8341 aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg atccggataa
     8401 cgccggcgaa gggatttgag gctccttctc aagtcctttg ccactctttt ttgaggtgca
     8461 aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt ttcgctaaac
     8521 ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct ggtccaaaga
     8581 tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg ctggaagatg
     8641 gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc gatgtggtgc
     8701 tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt gccaaaagcg
     8761 gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact ttcgtcccgt
     8821 ttgattacgg ctatttcgcc ttcgtctatg acaagaacaa actgaaaaac ccgccgcaaa
     8881 gccttaaaga attggttgag agcgatcaaa actggcgggt gatttatcag gatccgcgca
     8941 ccagtacgcc ggggctgggc ctgctgttgt ggatgcaaaa agtctatggc gatgacgccc
     9001 cacaagcctg gcaaaaactg gcgaagaaaa ccgtcacggt caccaaaggc tggagcgaag
     9061 cctacggcct gtttttaaaa ggtgaaagcg atctggtact gagttacacc acctctccgg
     9121 cttatcacat tctcgaagag aaaaaagata actacgccgc cgcgaacttt agcgaaggtc
     9181 actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg gagctggcgc
     9241 aaaaattcct ccagtttatg gtttctccgg cttttcagaa tgcgatccca accggtaact
     9301 ggatgtatcc ggtggcaaat gtcacgctgc ctgccgggtt tgaacaattg accaaaccgg
     9361 ccaccacgct ggagttcaca ccagctgaag tggcggcaca acgtcaggca tggattagcg
     9421 aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg taagcgccgc
     9481 cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga acgcgccgca
     9541 gggtaactgg gtggcagtct ggcaggacag ctacctgtgg catgtggtgc gcttctcctt
     9601 ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc tcgcccgcgc
     9661 cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg caatgacctt
     9721 gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc gccagggctg
     9781 gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct acggcctgca
     9841 aggtattttg ctggcgcacg tattttttaa tctgccgatg gcgagccgct tattactcca
     9901 ggcactggaa aacattcccg gcgaacaacg tcagcttgcc gcccagcttg ggatgcgcgg
     9961 ctggcatttt ttccgcttcg tcgaatggcc gtggttacgg cgacaaatcc cgccggttgc
    10021 tgcacttatc tttatgctct gtttcgccag cttcgccacc gtgctatcac tgggcggcgg
    10081 tccgcaggcg accactatcg agctggctat ttatcaggcg ctgagttacg actacgatcc
    10141 tgcccgcgcg gcgatgctgg cgctgatcca gatggtgtgt tgccttgggc tggtactgtt
    10201 gagtcagcga ttgagtaagg ccattgcgcc aggcaccacg ctgctgcaag gctggcgcga
    10261 cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaattgtgc tggcgctgct
    10321 gctgttgctg ccaccgttac tggcggtgat cgtcgatggc gtaaatcgcc agttgccgga
    10381 agtgctggca caaccggtgc tgtggcaggc gctgtggacc tcgttgcgta ttgcgctggc
    10441 ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc gcgaactgcg
    10501 ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt tgatcctcgc
    10561 catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca ctatcggcct
    10621 gccgcaatct gcggacggca ttgtgatttt caccaatgcg ttaatggcga tcccttatgc
    10681 gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagta tgttgtgtca
    10741 gtcgctgggc attgaaggct ggtcgcgctt aaaagtggtg gagcagcgcg ccctgaagcg
    10801 tccgctggcg caggcgctag cttttgcctg cgtgctgtcg attggtgatt ttggcgtggt
    10861 ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc agcaaattgg
    10921 ctcctatcgc agccaggacg gcgcggtcac cgcattaatt ctgctgctac tctgttttct
    10981 gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact gatatcacct
    11041 ggctttatca ccatttgccg atgcgtttta gcttaacggt agaacgcggc gagcaggtgg
    11101 caatcctcgg gccaagcggc gcgggtaaaa gtaccctgct gaatttgatt gccggttttc
    11161 tgacgccagc cagcggttcg ctgactatcg atggcgttga tcacaccacg acgccgccgt
    11221 cacgccgtcc ggtgtcgatg ctgtttcagg agaacaacct gttcagccac ctgacggtcg
    11281 cgcagaacat cgggctgggg ctaaatccgg ggttgaaact gaacgcggca cagcaggaga
    11341 aaatgcacgc gattgcccgt cagatgggga ttgataattt aatggcgcgg ttaccgggcg
    11401 agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta cgcgagcagc
    11461 cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc caggagatgt
    11521 tgacgctggt gagcacgagc tgccagcagc acaaaatgac gttattgatg gtgtcgcaca
    11581 gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac gggcgcatcg
    11641 cctggcaggg taaaaccgat gagttgttaa gcggtaaggc gagtgcttcg gcgctgttgg
    11701 gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga agggaacaaa
    11761 ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat atcgatatac
    11821 accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat ggcagaaatc
    11881 aacggcgtca gccacaacaa acggccgcgc gacaaataat gacttaaacg atcggttgct
    11941 ttaccgctgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa taccgccgtc
    12001 gccagcagca accatttaaa ctcgccgctc tgcattccgg ctggaatatc gatcgccgcg
    12061 cccgccagaa tcccaggcag gaagtaaaac ggtggccaca gcaggcagcc gataatattc
    12121 ggcgtaatga attttgccac cggcagatcc agcattcccg ccaccattgg caccagcgga
    12181 cgcgtcgggc caacaaagcg accgaccaga atggtgaaca tgctgtgttg atgcaacgca
    12241 tgttcagttt tatcgagtaa tgctttgttt ttcttcagaa atgaccagcg atgcaacggc
    12301 tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcagaca accgacaatc
    12361 cctgccagcc aggcgtgcca gaaacttaac tcgccgctgc caatcagcgc tcccagcccc
    12421 gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc cagaaaggcc
    12481 accaacacca ccgccatcaa tgaatacacg gtagattggg taataaagtg ttccagcaat
    12541 gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc tatgctactc
    12601 cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg ctacatcatt
    12661 cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa
    12721 tactcgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg
    12781 catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct cgcgccagct
    12841 taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca
    12901 aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta
    12961 ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc
    13021 catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc
    13081 ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc
    13141 ttcatccggg cgaaagaaac ccgtattggc aaatattgac ggccagttaa gccattcatg
    13201 ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg
    13261 acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac
    13321 aaattctcgt ccctgatttt tcaccccccc ctgaccgcga atggtgagat tgagaatata
    13381 acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg
    13441 cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg gatcattttg
    13501 cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt gtccatattg
    13561 catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc aaccggtaac
    13621 cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa
    13681 caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg gcgtcacact
    13741 ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac gctttttatc
    13801 gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg atggcgattg
    13861 caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggac tgcgccagcg
    13921 gtgaagagat cgccaccagc gtagaatggt atccccgttg gcagaaaggg caattttgtg
    13981 atgccccgaa taaccagttc cgtcatcatc cgcgtgacta cattgagtca atggaagcgg
    14041 cactgaaaac cgtgcttgca gagcttagcg tcgaacagcg cgcagctgtg gtcgggattg
    14101 gcgttgacag taccggctcg acgcccgcac cgattgatgc cgacggtaac gtgctggcgc
    14161 tgcgcccgga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa gaccacactg
    14221 cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac gttgactact
    14281 cccgctacat tggtggtatt tattccagcg aatggttctg ggcaaaaatc ctgcatgtga
    14341 ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg tgcgactggg
    14401 tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga cgttgcagcg
    14461 ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc agtttctttg
    14521 atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact gacacctgga
    14581 ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc ggcctgcctg
    14641 aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt ggcgcaggcg
    14701 cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt ctgattgccg
    14761 acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt gatggcagcg
    14821 tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat atctacgcct
    14881 ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat ccggaactga
    14941 aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa gcatgggcca
    15001 aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt cgtcgcacgc
    15061 caaacgctaa ccaacgcctg aaaggggtga ttaccgatct taacctcgct accgacgctc
    15121 cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca atcatggagt
    15181 gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc atcgcgcgga
    15241 aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg caaattgttg
    15301 cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc gcgaaagtgc
    15361 acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa accctgcaac
    15421 cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag caatgggcga
    15481 tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc caggccgttg
    15541 cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg gtttgtcatt
    15601 ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca tgccgagcac
    15661 gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt gaaaccgctg
    15721 ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga cgatcgttgc
    15781 gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat caacggcctg
    15841 accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc gctgccgtgg
    15901 gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg tcgcgagttc
    15961 ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca ctggcaggat
    16021 aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa acaggatacc
    16081 cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt caccgatggt
    16141 gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc ggttggcgat
    16201 ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt cgatgagtac
    16261 gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg acagaacgtg
    16321 ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg tggcttccac
    16381 gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg tctggccgta
    16441 cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa aaccgccgcc
    16501 ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc ctttatggag
    16561 gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca tatgctggaa
    16621 gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca tctcggtatt
    16681 ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc agcgattgtc
    16741 gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat cgacacggtg
    16801 aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa agcgcaaccg
    16861 gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca taccgtcttc
    16921 agccatgcgc tgaacctcaa cgatatgcgt cagttcgccg agatgcacaa cattgaaatc
    16981 acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg ctggaacgaa
    17041 gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaacgcct gatgcgacgc
    17101 tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc aaagcgcatc
    17161 cggcattcaa cgcctgatgc gacgctggcg cgtcttatca ggcctacgcg ctgcgatttt
    17221 gtagaccgga taagcgaagc gcatccggca cgaaggagtc aacatgttag aagatctcaa
    17281 acgccaggta ttagaagcca acctggcgct gccaaaacac aacctggtca cgctcacatg
    17341 gggcaacgtc agcgccgttg atcgcgagcg cggcgtcttt gtgatcaaac cttccggcgt
    17401 cgattacagc gtcatgaccg ctgacgatat ggtcgtggtt agcatcgaaa ccggtgaagt
    17461 ggttgaaggt acgaaaaagc cctcctccga cacgccaact caccggctgc tctatcaggc
    17521 attcccctcc attggcggca ttgtgcatac gcactcgcgc cacgccacca tctgggcgca
    17581 ggcgggtcag tcgattccag caaccggcac cacccacgcc gactatttct acggcaccat
    17641 tccctgcacc cgcaaaatga ccgacgcaga aatcaacggc gaatatgagt gggaaaccgg
    17701 taacgtcatc gtagaaacct ttgaaaaaca gggtatcgat gcagcgcaaa tgcccggcgt
    17761 tctggtccat tcccacggcc cgtttgcatg gggcaaaaat gccgaagatg cggtgcataa
    17821 cgccatcgtg ctggaagagg tcgcttatat ggggatattc tgccatcagt tagcgccgca
    17881 gttaccggat atgcagcaaa cgctgctgga taaacactat ctgcgtaagc atggcgcgaa
    17941 ggcatattac gggcagtaat gactgtataa aaccacagcc aatcaaacga aaccaggcta
    18001 tactcaagcc tggttttttg atggattttc agcgtggcgc aggcaggttt tatcttaacc
    18061 cgacactggc gggacacccc gcaagggaca gaagtctcct tctggctggc gacggacaac
    18121 gggccgttgc aggttacgct tgcaccgcaa gagtccgtgg cgtttattcc cgccgatcag
    18181 gttccccgcg ctcagcatat tttgcagggt gaacaaggct ttcgcctgac accgctggcg
    18241 ttaaaggatt ttcaccgcca gccggtgtat ggcctttact gtcgcgccca tcgccaattg
    18301 atgaattacg aaaagcgcct gcgtgaaggt ggcgttaccg tctacgaggc cgatgtgcgc
    18361 ctgccagaac gctatctgat ggagcggttt atcacctcac cggtgtgggt cgagggtgat
    18421 atgcacaatg gcactatcgt taatgcccgt ctgaaaccgc atcccgacta tcgtccgccg
    18481 ctcaagtggg tttctataga tattgaaacc acccgccacg gtgagctgta ctgcatcggc
    18541 ctggaaggct gcgggcagcg catcgtttat atgctggggc cggagaatgg cgacgcctcc
    18601 tcgcttgatt tcgaactgga atacgtcgcc agccgcccgc agttgctgga aaaactcaac
    18661 gcctggtttg ccaactacga tcctgatgtg atcatcggtt ggaacgtggt gcagttcgat
    18721 ctgcgaatgc tgcaaaaaca tgccgagcgt taccgtcttc cgctgcgtct tgggcgcgat
    18781 aatagcgagc tggagtggcg cgagcacggc tttaaaaacg gcgtcttttt tgcccaggct
    18841 aaaggtcggc taattatcga cggtatcgag gcgctgaaat ccgcgttctg gaatttctct
    18901 tcattctcgc tggaaactgt cgctcaggag ctattaggcg aaggaaaatc tatcgataac
    18961 ccgtgggatc gaatggacga aattgaccgc cgtttcgccg aagataaacc tgcgctggca
    19021 acttataacc tgaaagattg cgagctggtg acgcagatct tccacaaaac tgaaatcatg
    19081 ccatttttac tcgaacgggc aacggtgaac ggcctgccgg tggaccgaca cggcggttcg
    19141 gtggcggcat ttggtcatct ctattttccg cgaatgcatc gcgctggtta tgtcgcgcct
    19201 aatctcggcg aagtgccgcc gcacgccagc cctggcggct acgtgatgga ttcacggcca
    19261 gggctttatg attcagtgct ggtgctggac tataaaagcc tgtacccgtc gatcatccgc
    19321 acctttctga ttgatcccgt cgggctggtg gaaggcatgg cgcagcctga tccagagcac
    19381 agtaccgaag gttttctcga tgcctggttc tcgcgagaaa aacattgcct gccggagatt
    19441 gtgactaaca tctggcacgg gcgcgatgaa gccaaacgcc agggtaacaa accgctgtcg
    19501 caggcgctga aaatcatcat gaatgccttt tatggcgtgc tcggcaccac cgcctgccgc
    19561 ttcttcgatc cgcggctggc atcgtcgatc accatgcgtg gtcatcagat catgcggcaa
    19621 accaaagcgt tgattgaagc acagggctac gacgttatct acggcgatac cgactcaacg
    19681 tttgtctggc tgaaaggcgc acattcggaa gaagaagcgg cgaaaatcgg tcgtgcactg
    19741 gtgcagcacg ttaacgcctg gtgggcggaa acgctgcaaa aacaacggct gaccagcgca
    19801 ttagaactgg agtatgaaac ccatttctgc cgttttctga tgccaaccat tcgcggagcc
    19861 gataccggca gtaaaaagcg ctacgccggg ctgattcagg agggcgataa acagcggatg
    19921 gtgtttaaag ggctggaaac cgtgcgcacc gactggacgc cgctggccca gcagtttcag
    19981 caggagctat acctgcgcat cttccgcaac gagccatatc aggaatatgt acgcgaaacc
    20041 atcgacaaac tgatggcggg tgaactggat gcgcgactgg tttaccgtaa acgccttcgc
    20101 cgtccgctga gcgagtatca gcgtaatgtg ccgcctcatg tacgcgccgc tcgccttgcc
    20161 gatgaagaaa accaaaagcg tggtcgcccc ttgcaatatc agaatcgcgg caccattaag
    20221 tacgtatgga ccaccaacgg cccggagccg ctggactacc aacgttcacc actggattac
    20281 gaacactatc tgacccgcca gctacaaccc gtggcggagg gaatactccc ttttattgag
    20341 gataattttg ctacacttat gaccgggcaa cttgggctat tttgagcaaa aaaagagttc
    20401 gccagatacc attttgatgc gtgacgaatg ctttgccatc cagtaccata gcgccctttc
    20461 cattcctgga cctgaataac accactacct cataagcacg gtagcgggtg gttattgcct
    20521 gcaattaaag atatagagcc gaacacatat gccttttaca cttggtcaac gctggatcag
    20581 cgatacagaa agcgaattgg gacttggaac cgttgtcgcg gtggatgcgc gaactgtcac
    20641 tttacttttc ccatctactg gtgaaaaccg tctgtacgca cgcagtgatt cccccgtgac
    20701 ccgcgtgatg ttcaaccctg gtgataccat taccagccat gacggctggc agatgcaagt
    20761 cgaagaagta aaagaagaaa atggcttgct gacctatatc ggtactcgcc tggatactga
    20821 agagtccggc gtagccctgc gtgaagtttt ccttgatagc aaactggtgt tcagcaaacc
    20881 gcaggaccgc ctgtttgccg ggcagattga ccgtatggac cgctttgcgc tgcgttatcg
    20941 cgcgcgtaag tattccagcg aacagttccg tatgccgtac agcggcctgc gcggtcagcg
    21001 taccagcctg atcccgcacc agctcaacat cgctcatgat gttggccgcc gccacgcgcc
    21061 gcgcgtcctg ctggctgacg aagtgggttt agggaaaacc attgaagccg ggatgatcct
    21121 gcatcagcaa ctgctctctg gcgctgctga acgtgtgctg attatcgtcc cggaaacctt
    21181 acagcatcag tggctggtag aaatgctgcg ccgtttcaac ctgcgctttg ctctgtttga
    21241 tgatgagcgt tatgccgaag ctcagcacga tgcttacaac ccgttcgaca ccgaacagct
    21301 ggtgatttgc tcgctggatt ttgcccgtcg tagcaaacag cgcctggaac atctctgtga
    21361 agccgaatgg gacctgctgg tggtcgatga agcgcatcac ctggtgtgga gcgaagatgc
    21421 gccaagccgt gaatatcagg ccattgaaca actggcagag cacgtgccgg gcgttctgct
    21481 gctgaccgcg accccggaac agctggggat ggaaagccac ttcgcccgtc tgcgtctgct
    21541 ggacccgaac cgtttccacg attttgcgca gttcgttgaa gagcagaaaa attatcgtcc
    21601 ggttgcggac gccgttgcca tgctgctggc aggtaacaaa ctgagcaatg acgaactgaa
    21661 catgctcggc gagatgatcg gcgagcagga tatcgagccg ctgttgcagg cagcaaacag
    21721 cgacagcgaa gatgcccaga gcgcccgtca ggagctggtt tcgatgctga tggatcgcca
    21781 cggcaccagc cgcgtgctgt tccgtaacac gcgtaacggt gtgaaaggat tcccgaaacg
    21841 cgagctgcac accattaaac tgccgctacc gacgcagtat cagacggcta ttaaagtctc
    21901 cggcattatg ggcgcacgta aaagtgcgga agatcgtgct cgcgatatgc tctacccgga
    21961 gcgtatttat caggaatttg aaggtgataa cgccacctgg tggaacttcg atccgcgcgt
    22021 tgagtggctg atgggctacc tgaccagcca tcgctctcag aaagtgctgg tgatctgcgc
    22081 caaagctgcc actgcgctgc aactggagca ggtactgcgc gaacgtgaag gtattcgcgc
    22141 tgcggtgttc cacgaaggta tgtcgattat cgaacgtgac cgcgctgccg cctggtttgc
    22201 cgaagaagac accggcgcac aggtactgct gtgctcagaa atcggttctg aaggacgtaa
    22261 cttccagttc gccagccaca tggtgatgtt tgacctgcca ttcaacccgg atctactgga
    22321 gcagcgtatt ggtcgtctgg atcgtatcgg ccaggcgcac gatattcaga tccatgtgcc
    22381 ttatctggag aaaaccgctc agtcggtgct ggtgcgctgg tatcacgaag gtctggatgc
    22441 atttgagcac acctgcccga ccggacgcac tatttacgat agcgtataca acgatctgat
    22501 taactatctg gcttcaccgg atcaaaccga aggctttgac gatctgatca aaaactgccg
    22561 cgagcaacat gaagcgctga aagcacagct ggaacagggt cgtgaccgcc tgctggaaat
    22621 ccactccaac ggtggcgaaa aagcccaggc actggcagaa agcattgaag agcaggatga
    22681 cgataccaac ctgatcgcct tcgccatgaa cctgttcgat attatcggta tcaatcagga
    22741 cgatcgcggc gacaacatga tcgtgctgac gccgtccgat catatgctgg tgccggactt
    22801 ccctggcctg tcggaagatg gcatcaccat cacctttgat cgtgaagtgg cactggcgcg
    22861 tgaagatgca cagtttatta cctgggagca tccgctgatc cgcaacggtc tggatctgat
    22921 cctttctggc gataccggta gcagcacgat ttcactgtta aaaaacaaag cgttgccggt
    22981 aggtacgctg ttggtggaac tgatttatgt ggttgaagcc caggctccga agcagttgca
    23041 gctcaaccgc ttcctgccac cgacgccggt acgtatgctg ctggataaaa acggcaacaa
    23101 cctggcggcg caggtagagt ttgaaacctt taaccgccag cttaacgcgg ttaaccgtca
    23161 caccggcagc aaactggtta acgccgtgca gcaggatgtt cacgctatcc ttcaactggg
    23221 tgaagcgcag atcgagaaat ctgcccgtgc attgattgat gcagcgcgta acgaagccga
    23281 cgaaaaactg tctgccgagc tgtctcgtct ggaagctctg cgtgcagtga acccgaacat
    23341 tcgtgacgac gaactgaccg ccattgagag caaccgtcag caggtaatgg aaagcctgga
    23401 tcaggcaggt tggcgtctgg atgccctgcg tttgatcgtt gtaacgcatc agtaacggag
    23461 ccgaaaatgg ggatggaaaa ctacaatcca ccgcaggaac cctggttggt tatcctgtat
    23521 caggatgacc atattatggt ggtcaacaag ccgagcggtt tgttgtcagt gccgggtcgt
    23581 ctggaagagc acaaagacag cgtgatgacg cgcattcagc gtgattatcc gcaggcagaa
    23641 tcggtgcatc gtctggatat ggctaccagc ggcgtgattg tagtggcgct gaccaaagcc
    23701 gcggagcggg agttaaaacg ccagttccgc gagcgcgagc cgaaaaagca gtatgtggcc
    23761 cgcgtctggg ggcatccatc ccccgcagaa ggtctggtgg atctgccgct gatttgcgac
    23821 tggccaaacc gcccgaaaca gaaagtctgt tacgaaacgg gtaaacctgc gcagacggaa
    23881 tatgaagtgg tggagtatgc ggcggataac acggcaagag tggtgttaaa accgattacc
    23941 gggcgttcgc atcaattgcg tgtgcatatg ctggcgctgg ggcatccgat tctcggcgat
    24001 cgtttttatg catcaccaga agcgagagcg atggcaccgc atttgttgct acatgcagag
    24061 atgttgacga ttacccatcc ggcgtatggc aatagtatga cgtttaaagc gccagcggat
    24121 ttttaatcgt cacgctggcg cagttcggta ggcggataag gcgttcacgc cgcatcaggc
    24181 aattgtgcgc cgattgcctg atgcgacgcg ggccgcgtct tatcaggcct acagtttaaa
    24241 acttatttaa accctttctg ctgcttaatt agttcatagg cctgctgaat ttcctgcgct
    24301 ttctgcttcg ccatctccat catctcaggc ggcaaacctt tcgccaccag cttatcggga
    24361 tggtgttcac tcatcagctt acggtaggca cgtttgatgg tggtcgcatc atccgtcggc
    24421 ttcacgccca gcacattaca ggcatcttcc agcgtcgggc cacgctgcgc ttgctgccag
    24481 ttaccaccgc cagattgctg ctgataaccg ccgccaaact gtgcaccgcc ctgcatcatg
    24541 agcaaaaact ggtcaaactg ggcgcgggag atccctaatt cttcagcaat gacatacagc
    24601 accgcccgtt cattcgggtg cagtgaacca tcagcaaacg ccgcctgaat ctggatctcc
    24661 agaaacatac gaattaagtc aaaacgacca aagcagacac tgcgaaactg gcgcatcttt
    24721 tcgcgcagcg ggtaattatc tgattttccc acccggaacg cgttttgcgc cgcagtacgg
    24781 gaagcgccat gaaggttcat tcggtccatc aactggctgg cgatatgaat atcagcctcc
    24841 gtgacgcgac ctttggattt ggttaaatgc cccatcactt caaaagtggt ggcaaaaaac
    24901 agcgcctgac gctcacgctg gttggcgaac cacgccattt tacggctacg ggctttatca
    24961 aacatatgac caattaacag gcctaacact acgccccaaa agccgccgcc catcagtaag
    25021 gccacggcca cgccaatgat ttttccccaa tactgcatat attccccaaa tcgacacacg
    25081 gatatcaggg ctatctccca caatataaag gtgcttttac cgttttccgg ctgcggtcaa
    25141 ttgtgggaca tcgcctataa tttgcattat catacctgtc attcaatgcc gtgcctaaca
    25201 ccactgacgc tattcggaca ggattaacac tagcgtagag atgacgagta cgttagtctc
    25261 tgaccgtttg tcacgcgcaa cgttaccgat gatggaacaa taaaatcaac gtatgaaaaa
    25321 acgtatcccc actctcctgg ccaccatgat tgccaccgcc ctttatagtc aacagggact
    25381 ggcagccgac ctcgcctcac agtgcatgtt gggcgtgcca agctatgacc gtcctctggt
    25441 acagggcgat accaatgact tacccgtgac tatcaatgct gaccacgcga aaggggacta
    25501 cccggatgac gccgtgttta ctggcagcgt ggatatcatg cagggtaaca gccgtctgca
    25561 ggccgacgaa gtgcagctcc atcaaaaaga ggcaccagga caaccggagc cggtacgtac
    25621 cgttgatgcg ctcggtaatg tccattacga cgataaccag gtgatcctca aagggccgaa
    25681 aggctgggcg aatctgaaca ccaaagatac caacgtctgg gaaggtgatt accagatggt
    25741 gggtcgccag ggtcgcggta aagcggacct gatgaaacaa cgtggcgaaa accgctatac
    25801 cattctggat aacggtagct ttacctcctg tctgccgggt tctgacacct ggagcgtggt
    25861 aggtagcgaa attattcatg accgcgaaga acaagttgcg gagatctgga acgcccgctt
    25921 taaggtgggt ccggtaccga tcttttatag cccctatttg cagttgccgg tgggtgacaa
    25981 acgtcgctct ggtttcttga tcccgaacgc caagtacacc accaccaact actttgagtt
    26041 ctacctgcca tattactgga acatcgcgcc aaatatggat gccaccatca cgccgcatta
    26101 tatgcatcgt cgtggcaaca tcatgtggga gaacgaattc cgctacctct cccaggcggg
    26161 cgctggcttg atggaactgg actatctgcc ttcagataaa gtctatgaag atgaacaccc
    26221 gaacgatgac agttcacgtc gttggttatt ctactggaac cactccgggg tcatggatca
    26281 agtgtggcgt ttcaacgtcg actacaccaa ggtcagcgat cctagctact tcaatgattt
    26341 cgataacaag tacggttcca gtactgacgg ctacgcaacg caaaaattca gcgttggcta
    26401 tgcggtgcaa aacttcaatg ccaccgtttc aaccaagcag ttccaggttt tcagcgaaca
    26461 gaacaccagt agctactcgg cagagccgca gttagacgtt aattactacc agaatgatgt
    26521 tggtccgttt gatacgcgta tttacggcca ggcagtgcac tttgttaaca ccagagacga
    26581 catgcctgaa gcaacccgtg ttcacctgga accgaccatc aatttgccgc tctctaataa
    26641 ctggggcagc atcaataccg aagcgaagtt gctggcaacc cattatcagc aaaccaatct
    26701 tgactggtat aactccagaa acacgaccaa gctggacgaa tccgttaacc gcgtaatgcc
    26761 gcaattcaaa gttgacggca aaatggtctt tgaacgcgat atggaaatgc tggctccggg
    26821 ttatacccaa acgctggaac cgcgcgcgca gtatttgtac gtgccgtatc gcgatcagag
    26881 cgacatctat aactacgact cctctctgct gcaatctgac tactctggcc tgttccggga
    26941 ccggacttac ggcggtcttg accgtattgc ctccgctaac caggtgacga ccggtgtcac
    27001 atctcgcata tatgatgatg ctgccgttga acgttttaat atttccgttg gtcaaatcta
    27061 ctatttcacg gagtctcgca ctggcgatga caacataaca tgggagaatg acgacaaaac
    27121 gggctcactg gtgtgggcag gcgatactta ctggcgtatc tccgagcgtt ggggattgcg
    27181 tggcgggatt cagtacgata cacgtctgga taacgtagcg accagtaact ccagcattga
    27241 ataccgtcgg gatgaagacc gtctggtaca gctgaattac cgttacgcca gcccggaata
    27301 tattcaggct acgctgccta agtactattc cactgctgag caatataaga atggtatttc
    27361 gcaggtaggt gccgtcgcca gctggccaat tgccgatcgt tggtcaattg ttggggccta
    27421 ctactacgac accaatgcga acaagcaagc cgactctatg ttaggtgtgc aatacagctc
    27481 ctgctgctat gcaattcgcg tcggttacga gcggaagctg aacggttggg ataacgataa
    27541 acaacatgcg gtatatgaca acgcaatcgg ctttaacatc gaacttcgcg gcctgagctc
    27601 caactacggt ctgggtacgc aagagatgct gcgttcgaac attctgccgt atcaaaacac
    27661 tttgtgattt gttgatttac cacgtaatcc gcagtgcggt taattgaaat ggaaaaagta
    27721 tgaagaactg gaaaacgctg cttctcggta tcgccatgat cgcgaatacc agtttcgctg
    27781 ccccccaggt agtcgataaa gtcgcagccg tcgtcaataa cggcgtcgtg ctggaaagcg
    27841 acgttgatgg attaatgcag tcggtaaaac tgaacgctgc tcaggcaagg cagcaacttc
    27901 ctgatgacgc gacgctgcgc caccaaatca tggaacgttt gatcatggat caaatcatcc
    27961 tgcagatggg gcagaaaatg ggagtgaaaa tctccgatga gcagctggat caggcgattg
    28021 ctaacatcgc gaaacagaac aacatgacgc tggatcagat gcgcagccgt ctggcttacg
    28081 atggactgaa ctacaacacc tatcgtaacc agatccgcaa agagatgatt atctctgaag
    28141 tgcgtaacaa cgaggtgcgt cgtcgcatca ccatcctgcc gcaggaagtc gaatccctgg
    28201 cgcagcaggt gggtaaccaa aacgacgcca gcactgagct gaacctgagc cacatcctga
    28261 tcccgctgcc ggaaaacccg acctctgatc aggtgaacga agcggaaagc caggcgcgcg
    28321 ccattgtcga tcaggcgcgt aacggcgctg atttcggtaa gctggcgatt gctcattctg
    28381 ccgaccagca ggcgctgaac ggcggccaga tgggctgggg ccgtattcag gagttgcccg
    28441 ggatcttcgc ccaggcatta agcaccgcga agaaaggcga cattgttggc ccgattcgtt
    28501 ccggcgttgg cttccatatt ctgaaagtta acgacctgcg cggcgaaagc aaaaatatct
    28561 cggtgaccga agttcatgct cgccatattc tgctgaaacc gtcgccgatc atgactgacg
    28621 aacaggcccg tgtgaaactg gaacagattg ctgctgatat caagagtggt aaaacgactt
    28681 ttgctgccgc agcgaaagag ttctctcagg atccaggctc tgctaaccag ggcggtgatc
    28741 tcggctgggc tacaccagat attttcgatc cggccttccg tgacgccctg actcgcctga
    28801 acaaaggtca aatgagtgca ccggttcact cttcattcgg ctggcattta atcgaactgc
    28861 tggatacccg taatgtcgat aaaaccgacg ctgcgcagaa agatcgtgca taccgcatgc
    28921 tgatgaaccg taagttctcg gaagaagcag caagctggat gcaggaacaa cgtgccagcg
    28981 cctacgttaa aatcctgagc aactaatggt taaaacccaa cgtgttgtga tcactcccgg
    29041 cgagcccgcc gggattggcc cggacttagt tgtccagctt gcacagcgtg agtggccggt
    29101 cgaactggtt gtttgtgccg atgccactct ccttaccgac cgggcagcga tgctcggttt
    29161 gccgctcacc ctccgccctt attcccccaa ctcccctgca caaccgcaaa ctacgggcac
    29221 attaacgcta cttcctgtcg cgctacgtga atctgtcact gcggggcagt tagcgattga
    29281 aaatgggcat tacgtggtgg agacgctggc gcgagcgtgt gatggctgtc tgaacggtga
    29341 atttgctgcg ctgatcacag gccccgtgca taaaggcgtc attaacgacg caggcattcc
    29401 gtttaccggt cataccgagt ttttcgaaga gcgttcgcag gcgaaaaaag tggtgatgat
    29461 gctggcgacc gaagaacttc gcgtggcgct ggcaacgacg catttaccgc tgcgcgatat
    29521 cgcagatgct atcacccctg cacttttgca cgaagtgatt gctattttgc atcacgattt
    29581 gcgtacaaaa tttggtattg ccgaaccgcg cattctggtc tgcgggctga atccgcacgc
    29641 gggcgaaggc ggtcatatgg gtacggaaga gatagacacc attattccgg tgctcgacga
    29701 gctgcgggcg caggggatga aactcaacgg gccgctgcct gccgataccc tgtttcagcc
    29761 gaaatatctc gataacgccg acgccgtgct ggcgatgtac cacgatcagg gtcttcccgt
    29821 gctaaaatac cagggcttcg ggcgcggtgt gaacattacg ctgggcctgc cctttattcg
    29881 cacatcagtg gaccacggca ccgcgcttga actggcggga cgtggcgaag ccgatgtcgg
    29941 cagtattatt acggcgctta atctcgccat caaaatgatt gttaacaccc aatgaataat
    30001 cgagtccacc agggccactt agcccgtaaa cgcttcgggc aaaactttct caacgatcag
    30061 ttcgtgatcg acagcattgt ttctgccatt aacccgcaaa agggccaggc aatggtcgaa
    30121 atcggccccg gtctggcggc attgaccgag ccggtcggcg aacgtctgga ccagctgacg
    30181 gtcatcgaac ttgaccgcga tctggcggca cgtctgcaaa cgcatccatt cttaggcccg
    30241 aaactgacga tttatcagca ggatgcgatg acctttaact ttggcgaact ggccgagaaa
    30301 atgggtcagc cgctgcgtgt tttcggcaac ctgccttata acatctccac gccgttgatg
    30361 ttccatctgt ttagctatac tgatgccatt gccgacatgc actttatgtt gcaaaaagag
    30421 gtggtgaatc gtctggttgc aggaccgaac agcaaagcgt atggtcgatt aagcgtcatg
    30481 gcgcaatact attgcaacgt gatcccggta ctggaagttc cgccgtcagc ctttacacca
    30541 ccacccaaag tggattccgc cgtcgtgcgc ctggttcctc atgcaacgat gcctcacccg
    30601 gttaaagatg ttcgtgtgtt gagccgcatc accaccgaag cctttaacca gcgtcgtaaa
    30661 accattcgta acagcctcgg caacctgttt agcgtcgagg tgttaacggg aatggggatc
    30721 gacccggcga tgcgagcgga aaatatctct gtcgcgcaat attgccagat ggcgaactat
    30781 ctggcggaga acgcgccttt gcaggagagt taacgatgat caattcgccc cgagtgtgta
    30841 ttcaggttca aagcgtctac attgaggcac aatcttcacc tgataatgaa cgttacgttt
    30901 ttgcttatac cgtaaccata cgcaatctgg ggcgagcgcc agtgcagttg ttggggcgtt
    30961 actggctgat caccaatggc aatggccgtg aaaccgaagt ccagggcgaa ggagtggttg
    31021 gcgtccagcc acttatcgcg cctggcgaag agtaccagta caccagcggt gcaatcattg
    31081 aaaccccgct gggcaccatg cagggtcact acgaaatgat cgatgaaaat ggcgtccctt
    31141 tcagcatcga cattcccgta ttccgactcg ccgttcccac actcattcat taaaagaata
    31201 tggcgacata ccttattggc gacgttcatg gttgttacga tgaactgatc gcattgctgc
    31261 ataaagtaga atttacccct gggaaagata ccctctggct gacgggcgat ctggtcgcgc
    31321 gcggcccggg ttcgctggat gttctgcgct atgtgaaatc cttaggcgac agcgtacgtc
    31381 tggtgctggg caatcacgat ctgcatctgc tggcggtatt tgccgggatc agccgcaata
    31441 aaccgaaaga tcgcctgaca ccgctgctgg aagcgccgga tgccgacgag ctgcttaact
    31501 ggctgcgtcg ccagcctttg ctgcaaatcg acgaagagaa aaagttggtg atggcccacg
    31561 cagggatcac gccgcagtgg gatctacaga ccgccaaaga gtgcgcgcgc gatgtagaag
    31621 cggtgctatc gagtgactcc tatcccttct ttctcgacgc gatgtacggc gatatgccaa
    31681 ataactggtc accggaattg cgggggctgg gaagactgcg ttttatcacc aacgccttta
    31741 cccgtatgcg tttttgcttc ccgaacggtc aactggatat gtacagcaaa gaatcgccgg
    31801 aagaggcccc tgccccactg aaaccgtggt ttgcgattcc tggccctgtc gctgaagaat
    31861 acagcatcgc ctttggtcac tgggcatcgc tggagggcaa aggtacgccg gaaggtatat
    31921 acgcgctgga taccggctgc tgctggggtg gtacattaac ctgcctgcgc tgggaagata
    31981 aacagtattt tgtccagccg tcgaaccggc ataaggattt gggcgaagcg gcggcgtctt
    32041 aaacacagcc tgatatagga aggccggata agacgcgacc ggcgtcgcat ccggcactag
    32101 ccgtaaattc tatacaaaat taccgccgct ccagaatctc aaagcaatag ctgtgagagt
    32161 tctgcgcatc agcatcgtgg aattcgctga ataccgattc ccagtcatcc ggctcgtaat
    32221 ccgggaaatg ggtgtcgcct tccacttctg cgtcgatatg cgtcagatac agtttttgcg
    32281 cttttggcaa gaactgttca taaacgcgac cgccgccaat caccatgatt tctggtacgt
    32341 caccacacgc cgcgatggct tcatccaccg acttcaccca cgttacgcga tcgtccgtac
    32401 ccggttgact gctgaggata atatttttgc gtcctggcaa cggacgaccg attgattccc
    32461 aggtatggcg gcccataatc acgggtttat ttaaggtgtt gcgtttaaac caggcgagat
    32521 cggcaggcag gttccacggc atggcgtttt ccatgccgat aacgcgatct accgctaacg
    32581 ccgcaatcag actgatcatt gagatttccc gataaaaaaa ttgtcgccac tatacgtaaa
    32641 gcgtaaaccg tcgtcgactg gtgcgaggat gatgttgagg aaaattttat attctgctgg
    32701 cgagtccacg ctctctccct ggactcgccg cattacaatg aaacaaaaac aaacagttag
    32761 ctgtaaagtg tggtttacgt cactctttat taggatgagg gtttcgtttc cggttcatcc
    32821 gccatgttgc cggtatgttt accttcttcg gttccctgcc agccatgtcg ttgaattaat
    32881 gacagatgtt cgcggtcctc ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag
    32941 accgccgcgc gggctttggt gtcgttctca accattgcca tctcttccac catctgaata
    33001 ttaaagcggc ggaacacatc ggcacgttct cgcgcttcat acggccccag acctaaactt
    33061 tccagtgcca gacgcccggt tttcagcgca ccttcgaagg tttcacgctc cggcttttca
    33121 acgcctgcct gacgcaaacg aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc
    33181 aaatgcggga aatgttcttt caccatctct gtcagttgca ggttggtttg cggatcgtcg
    33241 atggcgttaa tcagcacttc cgctttcgcc gctccggcag attccagtaa atccatccgc
    33301 gtggcatcgc cataaaacac tttcatacca aatttacgca aggtttcgat atggtccgga
    33361 tcgtgatcga gcaccaccat tttcaccccg ctggagagca gtaaacgtcc ggtaatctgc
    33421 ccaaaacgac cgaatccggc gataatcacg cgcggctgtt cttcgtcgat ctcatcggct
    33481 tcacgcgctt cctcagtaga agattgctca aggcgattga ggatcaccag cagaatcggc
    33541 gttgctgcca tcgacagcgc caccgccagg gtcagcgatt tcgcccactc cggctccagc
    33601 acattcgcca tttgcgccgc accaaatacc acaaaggcaa actcactgcc ctgccctaac
    33661 agcaccgcaa accagcgacg ctgtttattt ggcacctgca acggtcgggc aatcagccac
    33721 agcatggcga ttttgatgat gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt
    33781 tcaagcagcg tgccaaagtc tatcgacatg ccaacaccga tgaaaaacag ccccagcagc
    33841 aaacctttaa atggttcgat atcactctcc agcgcatgac ggtattccga gcttgccagc
    33901 agtacgcccg ccagaaacgc gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa
    33961 ccaaagccaa acacgaggaa taacgccacg gcactaaaca cttcccgcaa gccagagcgg
    34021 gcaacaaaac gcagcgccgg acgcgtgaca tagcgcccca gcagtaccac cagcaccagc
    34081 gcgcccgcca cttttaacgc cgagacagca aatgcgccca tcgtcgtcga ggcactgctc
    34141 gctgccagta gcgggatcat cgccaccagc gggattgccg cgatatcctg gaacagcagc
    34201 accgcaaagg cactgcggcc catttgcgtc accatcagat tgcgttcatt catcgcctgc
    34261 atggcaatcg ccgttgatga gagcgccagc gtcatgccga tcagttccgc gacctgccag
    34321 cgcagcccaa gtaacatgca gaacagcccc agcaggccac cgcaaatcac catctgcaat
    34381 gcgccgccgc cgaacactgc cgcacgcagc ttccacagcc tttgtggatc gagttcgagg
    34441 ccgataataa acagcatcag caccacccca atctcggcaa agtgcagaat agattcggca
    34501 tcggtcacca gtcgcagccc ccacgggcca ataatgcagc cggcgatcag gtagccaagt
    34561 accgatccca gaccaagacg taccgcaatg ggtacaatca gcgctgccga accgagataa
    34621 atcagcgcct gaatcagcgt atggctatcc atgatgggcc tcctgccatt ccagcagacg
    34681 ttgcttatag tgacgcgcct gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg
    34741 catggcaaac ggtggcagcc agttcagccc gcagtagatt gccgtcgcct gtagcggctg
    34801 cgacagcaca tcaaagcccg gatgcgcacc aatttcaaaa tggctttccc cgccgccggt
    34861 cgtcaccgcc cacagcaaat gtttgccatg cagcgccgtg ccgccgtgac cgtaagccca
    34921 gccgtgcgag aaaactttat cgatccaaag tttgaggagc ggtggaatgc tgtaccactg
    34981 catcgggtgc tgccagacga tcagatcggc gcgagacagc gcctcctgct cggcggcaat
    35041 atcgatattg aagtcaggat agagttgata aagagagcga atttcgacgc cttccagcgt
    35101 ccttgcctgt tcaagcatcc gtttattcgc atgggaatga tgcggatacg gatgcgcata
    35161 aattataaga atcataggta gcccgcgtga ttacgccttt gttttcccac aaagtgtagt
    35221 catatattca cgcggcttat agtcaataat attgattcag aaaagagaga aatctgaaca
    35281 atttacccca ttctctgccc gcggatcgtc atattgctgg tttgcgccag tgacatccct
    35341 cgagtctccg gggcaaacgc tacggaaatc aacaagccaa acagcgagat acccgccccc
    35401 attagcatcg tgttactgat accgtaatta ttgataaaga tcggcagtgc ccaagtcgaa
    35461 acaatggtgc caatacggct taaggacata atcacgccca cggcagaggc gcggatatct
    35521 gtcgggaaga gttcattagg atagagccac tgcaaattac ccgacccgcc agagaaaaag
    35581 gcatacaccg caaaggccat cactaccagc cagatcccca tatccgggat cagccccaaa
    35641 accgccagcg ccagcgtcat catggcaaag ctgccaatca acaatggacg ccgtccggca
    35701 gtgtttaacc acagcatcgg cggaatacag ccgagcataa agaacaggct aatcaccacg
    35761 ttccccagcg ccgcgttttt gccaacaccc aatcccaaca aaccaacgat ttgcgggcca
    35821 aaggtgtaaa tggcgaacat tgggatcacc tggcaggtcc agatggcggc aacaaacaga
    35881 acaaaaggaa aatggcggcg attaaacaga tcgcgaaaac gggtttgctg cggctgctct
    35941 tcatcgaaag ccaccgcttc gccaaacagt tttatcatca tctcttcgca ctcttttact
    36001 cgccctttgc gtaataacca gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc
    36061 aacaaacagg ggatcgccgc gctacccagc atccagcgcc agccgccttc cacatcataa
    36121 agccagtagc cgaccagatc ggcgcaggtc gcgccgacat accacatggc ggcgataaag
    36181 ctgatggaaa atgcccgctg acgggtactg gagaactcgg tgatcattga ggtggcgatg
    36241 ggataatctg caccgatgac aatgccgata agtacccgca tcaccaacag ttcgacgggg
    36301 gatgaaacaa acatcgtcgc caccgatatc acgccgatgg cgatgatatc aatgaggaac
    36361 attttgcgcc gtccgacttt atcggagata tagccaaaca gcgatgtgcc aacgaacagc
    36421 ccggcgagcg ttcccgcgcc cagcaagcca atccagtcag cgtccagttt cagcgctggg
    36481 gtcagttgct ccagcgccac gccaatcatt accagtacat aaccatccag aaacggacca
    36541 ccgcttcccc acagcaaaat gcggcggtga atagaggaga atttgagatc gtcaaagttt
    36601 ctggacggtt gcatgggtta ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt
    36661 atcaaaccaa catcagccgt aacggaactc cacaccaaag gtgccgcgcg ggtattccca
    36721 ctgttccagc gccgacccca gccccagaat gcgacaggtg ccgcactcca gacatccggc
    36781 gtaatcgaag cgcacactgc cgtcatcctg cttcttgtat agacctgcgg ggcacgcttt
    36841 caccagcagc tccagggcct gtttatcggc atcggccttc acaacaatgt gtggatgctc
    36901 ttcatcgaca ttgaatttat tgacgcccag tttgacgtcc acattgacgg gagaagtcat
    36961 aaaacggtca ctcctttcat gccatccttg atcagattga tgaagcccac tttcttgccg
    37021 tggcggagga ttttcttgcg catcagttcc ggcgcactgc cgtcaatggt gaacaggtca
    37081 cgcgccacgc ccaccgccag ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag
    37141 gccggtagtt tctggtacat acgcatatcg cgcagcgggc cactctcaag atgctgacga
    37201 tattccgcga gtttttgctt actgaaatcg tcgcttttca tcgctgaaag cacggttttt
    37261 gctgcagctt ccccggcggc aatcgccaga tccataccgc gaatggtaaa accgaggttc
    37321 atacacattc cggcggcatc accggcaatc aatacgccgt caccaaccaa ctccggcagc
    37381 atgttgatgc ctgcttccgg cactacgtga gcggaatatt ccaccagctt gccgcccgcg
    37441 atcagcggtg ccactgccgg atgctgtttg aaatcttcca gcatttgcgg caccgatttt
    37501 tttgcgtcat gcagatgatg cagaccacaa accagcccca gcgacagggt attttcattg
    37561 gtataaagga agccgccgcc catcaggcca tcggtgggtg atcccgcaaa cagacaagcc
    37621 gcgccctgat taccctgcaa ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc
    37681 agttccttca cgccaaccgc cacatccgtc ggtttaacgc gttttgccat ccccagcttt
    37741 tcggcaagga tggagttcac tccatcagca aggatcaccg ttttcgcttc aatcacatcg
    37801 ccatcggctt ctacaccgac gactttgcca tcgcgctgta cgaggttatc gacgcggatc
    37861 ccggtaatta actgcgcgcc cgcttcttcg gcctgctcca tcagccaggc atcaaattta
    37921 ctgcgcaaaa cggagtaaga acgctgggat ggcgaggttt cgtcaccatt gcagtagtcc
    37981 atagtcatcg ctgacttttc cgtcataaac gcgagttttt catgggtgat caggcgttct
    38041 acgggggcgg agtcggcgaa accaggaata atgtgttcca ggctgtgggc atagagacgc
    38101 ccgccggtga cgttcttggc acctgcggaa ttgccacgct cgataactaa cacttgcgcc
    38161 ccttcgcggg cgagcaccag tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg
    38221 gcgtcaaaga tatcttcgga cataagagct ccctgtcagc ggcagggcca aaatgcagcc
    38281 ctgccagagt ggatcaacgc gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt
    38341 caccaacaat gccgtaatcc gcgtactgga agatcggcgc atttttatct ttattgatgg
    38401 cgaaaatggt ttgcgatgca ttagcaccaa ccatgtgctg gatctgcccg gagatcccca
    38461 ccgccaggta cagttcaggt ttcagcatca ggttggagat accgacatag cgttcgtgct
    38521 ccatccattt ttcgttttcc gccaccggac gagaacaggc caactccgca cctatcgcct
    38581 tgcaaagctg ttctgccagc gcaatgttct ctttgctgcc aataccgcga ccgacgctga
    38641 ccaccagacg ggctttgtcg agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc
    38701 gggtaatcgc cacagccgga gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg
    38761 cgtctggctg agccgcatcg aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg
    38821 caatgcgttc ttcgccaatc gccagaccac cgtaaaccat gtgtttcact gtcgctttac
    38881 cgtcctgtac gctgacggtg ctggcatcgt tagacaccgc cgctttaagg cgataaccca
    38941 gttttgccgc cagtaatttg ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc
    39001 cgtgctggcg aatagtgtca gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg
    39061 gtttgccgtt taatttccag acatgattag cgccgagctg gattgcctgt acgccgtcgg
    39121 catcattgag gacaaaggtg ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt
    39181 ccggcagacg agaaggggta tcgctgaata cccagacttg agaaaacgtg ttcatagcat
    39241 cccctgtaat taaatgactt tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc
    39301 gtcgccttca atcacgatgc gctgacgttc gcgctgtttc ggcgcagcaa cctgttgttc
    39361 agaccaggcg tccacggcgt taaaaccgat atccgccgcc gaccatacct ggactggctt
    39421 tttcgccgcg ccgagaatgg ctttcatcga aggaatttgt ggggagttga tatcagtgga
    39481 aacagcaaca accgcaggca gcggaatgct taaggtttcg gtttcatctt ccagttcgcg
    39541 ctcaacggtg agggtatctg ccgtcaggga gataattttg ctgacgccgt taactgccgg
    39601 aatattgagg atttcgccca ccagcagacc aacctgctgg gcataaaggt cggaagaacc
    39661 atcgccacag aggatcaaat caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc
    39721 cgtttgttgc ggcagtgcct gctcgaactg gtcatcaatc accacaatca gttcatccgg
    39781 gccgcgcgat agcacatctt tacgcccttt ggcgttggtc agggctttac cgcccacact
    39841 taaggccgtc acctgcgcct ctgctgcctg ttgctttagc tggcaagccg cttcaatagc
    39901 gttcagatcg tattggctta ttttggcatc ggctttgctg aagtctaatg aaccatcagc
    39961 attattgacc gcaatatcct gttcatcagg cacgcactta tagcaagtaa taatcttcat
    40021 tgcatctcca gaaatcatga aggaaacgtc gttcatatcg gtggtgttaa ttacagatat
    40081 cacgcccttt aatttggaaa taaaacttta atcaccaata ttgaaaatgt cacacgcaga
    40141 taaatccaac tttcaatatt gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa
    40201 aagaaatttt caatattgct ttatggatca ccaatattga aagtcagatg ctattttttt
    40261 actcacaaca caacataaca aactgattaa taatcaaaat taacgagaaa agcgccctct
    40321 attcacccga aacaaaaatg tgataacaat cacagaatgc agcttattga atacccatta
    40381 tgagttagcc attaa
//
LOCUS       DAZKHC020000036        39611 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZKHC020000036 DAZKHC020000000
VERSION     DAZKHC020000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.036"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(44..901)
                     /gene="murI"
                     /locus_tag="ACHOSM_RS10860"
                     /old_locus_tag="pgaptmp_002172"
     CDS             complement(44..901)
                     /gene="murI"
                     /locus_tag="ACHOSM_RS10860"
                     /old_locus_tag="pgaptmp_002172"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGU4558688.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPGAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(846..2690)
                     /gene="btuB"
                     /locus_tag="ACHOSM_RS10865"
                     /old_locus_tag="pgaptmp_002173"
     CDS             complement(846..2690)
                     /gene="btuB"
                     /locus_tag="ACHOSM_RS10865"
                     /old_locus_tag="pgaptmp_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HGU4558689.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNQGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2755..2945)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3059..4159
                     /gene="trmA"
                     /locus_tag="ACHOSM_RS10870"
                     /old_locus_tag="pgaptmp_002174"
     CDS             3059..4159
                     /gene="trmA"
                     /locus_tag="ACHOSM_RS10870"
                     /old_locus_tag="pgaptmp_002174"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HGU4558690.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4199..4558)
                     /gene="yijD"
                     /locus_tag="ACHOSM_RS10875"
                     /old_locus_tag="pgaptmp_002175"
     CDS             complement(4199..4558)
                     /gene="yijD"
                     /locus_tag="ACHOSM_RS10875"
                     /old_locus_tag="pgaptmp_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HGU4558691.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4558..5208)
                     /gene="fabR"
                     /locus_tag="ACHOSM_RS10880"
                     /old_locus_tag="pgaptmp_002176"
     CDS             complement(4558..5208)
                     /gene="fabR"
                     /locus_tag="ACHOSM_RS10880"
                     /old_locus_tag="pgaptmp_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HGU4558692.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5539..6939
                     /gene="sthA"
                     /locus_tag="ACHOSM_RS10885"
                     /old_locus_tag="pgaptmp_002177"
     CDS             5539..6939
                     /gene="sthA"
                     /locus_tag="ACHOSM_RS10885"
                     /old_locus_tag="pgaptmp_002177"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HGU4558693.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(6922..7839)
                     /gene="oxyR"
                     /locus_tag="ACHOSM_RS10890"
                     /old_locus_tag="pgaptmp_002178"
     CDS             complement(6922..7839)
                     /gene="oxyR"
                     /locus_tag="ACHOSM_RS10890"
                     /old_locus_tag="pgaptmp_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HGU4558694.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8105..9478)
                     /gene="argH"
                     /locus_tag="ACHOSM_RS10895"
                     /old_locus_tag="pgaptmp_002179"
     CDS             complement(8105..9478)
                     /gene="argH"
                     /locus_tag="ACHOSM_RS10895"
                     /old_locus_tag="pgaptmp_002179"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGU4558695.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQVAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9539..10315)
                     /gene="argB"
                     /locus_tag="ACHOSM_RS10900"
                     /old_locus_tag="pgaptmp_002180"
     CDS             complement(9539..10315)
                     /gene="argB"
                     /locus_tag="ACHOSM_RS10900"
                     /old_locus_tag="pgaptmp_002180"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HGU4558696.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10323..11327)
                     /gene="argC"
                     /locus_tag="ACHOSM_RS10905"
                     /old_locus_tag="pgaptmp_002181"
     CDS             complement(10323..11327)
                     /gene="argC"
                     /locus_tag="ACHOSM_RS10905"
                     /old_locus_tag="pgaptmp_002181"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HGU4558697.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11481..12632
                     /gene="argE"
                     /locus_tag="ACHOSM_RS10910"
                     /old_locus_tag="pgaptmp_002182"
     CDS             11481..12632
                     /gene="argE"
                     /locus_tag="ACHOSM_RS10910"
                     /old_locus_tag="pgaptmp_002182"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HGU4558698.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            12984..15635
                     /gene="ppc"
                     /locus_tag="ACHOSM_RS10915"
                     /old_locus_tag="pgaptmp_002183"
     CDS             12984..15635
                     /gene="ppc"
                     /locus_tag="ACHOSM_RS10915"
                     /old_locus_tag="pgaptmp_002183"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGU4558699.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEETIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     EDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            15818..17551
                     /gene="eptC"
                     /locus_tag="ACHOSM_RS10920"
                     /old_locus_tag="pgaptmp_002184"
     CDS             15818..17551
                     /gene="eptC"
                     /locus_tag="ACHOSM_RS10920"
                     /old_locus_tag="pgaptmp_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU4558700.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17766..18617
                     /gene="yijO"
                     /locus_tag="ACHOSM_RS10925"
                     /old_locus_tag="pgaptmp_002185"
     CDS             17766..18617
                     /gene="yijO"
                     /locus_tag="ACHOSM_RS10925"
                     /old_locus_tag="pgaptmp_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4558701.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18604..18945)
                     /gene="frwD"
                     /locus_tag="ACHOSM_RS10930"
                     /old_locus_tag="pgaptmp_002186"
     CDS             complement(18604..18945)
                     /gene="frwD"
                     /locus_tag="ACHOSM_RS10930"
                     /old_locus_tag="pgaptmp_002186"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU4558702.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18947..19825)
                     /gene="pflC"
                     /locus_tag="ACHOSM_RS10935"
                     /old_locus_tag="pgaptmp_002187"
     CDS             complement(18947..19825)
                     /gene="pflC"
                     /locus_tag="ACHOSM_RS10935"
                     /old_locus_tag="pgaptmp_002187"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HGU4558703.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETARDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(19791..22088)
                     /gene="pflD"
                     /locus_tag="ACHOSM_RS10940"
                     /old_locus_tag="pgaptmp_002188"
     CDS             complement(19791..22088)
                     /gene="pflD"
                     /locus_tag="ACHOSM_RS10940"
                     /old_locus_tag="pgaptmp_002188"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU4558704.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTNSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAERLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22139..22459)
                     /gene="frwB"
                     /locus_tag="ACHOSM_RS10945"
                     /old_locus_tag="pgaptmp_002189"
     CDS             complement(22139..22459)
                     /gene="frwB"
                     /locus_tag="ACHOSM_RS10945"
                     /old_locus_tag="pgaptmp_002189"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU4558705.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22474..23553)
                     /gene="frwC"
                     /locus_tag="ACHOSM_RS10950"
                     /old_locus_tag="pgaptmp_002190"
     CDS             complement(22474..23553)
                     /gene="frwC"
                     /locus_tag="ACHOSM_RS10950"
                     /old_locus_tag="pgaptmp_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HGU4558706.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     SSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            23862..26363
                     /gene="ptsP"
                     /locus_tag="ACHOSM_RS10955"
                     /old_locus_tag="pgaptmp_002191"
     CDS             23862..26363
                     /gene="ptsP"
                     /locus_tag="ACHOSM_RS10955"
                     /old_locus_tag="pgaptmp_002191"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4558707.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26375..27037
                     /gene="fsa"
                     /locus_tag="ACHOSM_RS10960"
                     /old_locus_tag="pgaptmp_002192"
     CDS             26375..27037
                     /gene="fsa"
                     /locus_tag="ACHOSM_RS10960"
                     /old_locus_tag="pgaptmp_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU4558708.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            27048..28151
                     /gene="gldA"
                     /locus_tag="ACHOSM_RS10965"
                     /old_locus_tag="pgaptmp_002193"
     CDS             27048..28151
                     /gene="gldA"
                     /locus_tag="ACHOSM_RS10965"
                     /old_locus_tag="pgaptmp_002193"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HGU4558709.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            28425..29042
                     /gene="yijF"
                     /locus_tag="ACHOSM_RS10970"
                     /old_locus_tag="pgaptmp_002194"
     CDS             28425..29042
                     /gene="yijF"
                     /locus_tag="ACHOSM_RS10970"
                     /old_locus_tag="pgaptmp_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HGU4558710.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(29069..29974)
                     /gene="yijE"
                     /locus_tag="ACHOSM_RS10975"
                     /old_locus_tag="pgaptmp_002195"
     CDS             complement(29069..29974)
                     /gene="yijE"
                     /locus_tag="ACHOSM_RS10975"
                     /old_locus_tag="pgaptmp_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HGU4558711.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(30068..32248)
                     /gene="katG"
                     /locus_tag="ACHOSM_RS10980"
                     /old_locus_tag="pgaptmp_002196"
     CDS             complement(30068..32248)
                     /gene="katG"
                     /locus_tag="ACHOSM_RS10980"
                     /old_locus_tag="pgaptmp_002196"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HGU4558712.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32577..33467)
                     /gene="metF"
                     /locus_tag="ACHOSM_RS10985"
                     /old_locus_tag="pgaptmp_002197"
     CDS             complement(32577..33467)
                     /gene="metF"
                     /locus_tag="ACHOSM_RS10985"
                     /old_locus_tag="pgaptmp_002197"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HGU4558713.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            33543..33677
                     /locus_tag="ACHOSM_RS10990"
                     /old_locus_tag="pgaptmp_002198"
     CDS             33543..33677
                     /locus_tag="ACHOSM_RS10990"
                     /old_locus_tag="pgaptmp_002198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4558714.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(33816..36248)
                     /gene="metL"
                     /locus_tag="ACHOSM_RS10995"
                     /old_locus_tag="pgaptmp_002199"
     CDS             complement(33816..36248)
                     /gene="metL"
                     /locus_tag="ACHOSM_RS10995"
                     /old_locus_tag="pgaptmp_002199"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HGU4558715.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPTHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36251..37411)
                     /gene="metB"
                     /locus_tag="ACHOSM_RS11000"
                     /old_locus_tag="pgaptmp_002200"
     CDS             complement(36251..37411)
                     /gene="metB"
                     /locus_tag="ACHOSM_RS11000"
                     /old_locus_tag="pgaptmp_002200"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGU4558716.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKEPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37688..38005
                     /gene="metJ"
                     /locus_tag="ACHOSM_RS11005"
                     /old_locus_tag="pgaptmp_002201"
     CDS             37688..38005
                     /gene="metJ"
                     /locus_tag="ACHOSM_RS11005"
                     /old_locus_tag="pgaptmp_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HGU4558717.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38065..38673
                     /gene="yiiX"
                     /locus_tag="ACHOSM_RS11010"
                     /old_locus_tag="pgaptmp_002202"
     CDS             38065..38673
                     /gene="yiiX"
                     /locus_tag="ACHOSM_RS11010"
                     /old_locus_tag="pgaptmp_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HGU4558718.1"
                     /translation="MKYRLLPLSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            38818..39493
                     /locus_tag="ACHOSM_RS11015"
                     /old_locus_tag="pgaptmp_002203"
                     /pseudo
     CDS             38818..39493
                     /locus_tag="ACHOSM_RS11015"
                     /old_locus_tag="pgaptmp_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         9681 a        10968 c         9955 g         9007 t
ORIGIN      
        1 ggaaatttaa aataattttc tgaccgcgca acattcaacc aaatcagcct aaaactgcca
       61 gtttttcgag cgtttcgaag ccgtaacgct gtaaaacggg caataattgt tcagctcctg
      121 gcgtcattgc catacaaaag gcaatattcg catcggcaga ttttgcatcc ggggcttcat
      181 gttctaacaa ccaggccgtt cggcgagcaa tcgctgcgcc agaatccacc agccgggttc
      241 cctctggcag cacttgtaac agttcttctt gtagtagagg gaaatgggtg caacccaata
      301 caacggtatc tggcggctct ttcattctta accacgggcg taggatacgt tttagtgcat
      361 ccagagaaac atcttcgcca tgtagcttcg cttcagccaa ctcaaccatc tctgccgagc
      421 ccagcatttc tatctggcat tcattagcga aacgcgcgat cagctcatga gtataagaac
      481 gtttaactgt tccgcgggtt gccagtaatc cgacaatgcc atttgccgtc agacgtgcag
      541 caggtttaat cgccggcacg acaccaacaa ccgggaagtc gaacttttcg cgtaatgcag
      601 gaagtgaaac ggtactggca gtgttgcaag cgaccacagc cagcgcaagg ggataacgtt
      661 cttgcaccgc ggtgacaatt gccaccactc gctcaacaat aaacgcttcg cttttttcgc
      721 catacgggaa agcgacgtta tcgaaagcat aaatgtaatg gagatccggt aagagatgcc
      781 ggatctcgtc atagaccgac aacccaccga cgccggagtc aaacaccagc acggtgggac
      841 gtggttcaga aggtgtagct gccagacaag gtgtattccc gtcctgcagt ttggtagcca
      901 tagactgtct cataatcttt gtcgaacagg ttggctattt taccacgaac tgtcaggtga
      961 gaggtgaccg gatacgcaac cgcaagatcc cacaagctca caccgcccat tttaacggtt
     1021 tgataaggat aagatgagta atccttatca tagcgagtgc ctaaatactg ataagtaata
     1081 ccccagtcga agtcatacaa ctgccagtcg agctggtatt tcacctgctg tttagcacgg
     1141 cgtaacaacg gcgtgtcggt aatcgcattg cgcgcatcga cataatcata actcacagta
     1201 tgcgtcagtg gtccggtatc aaaattggcg gtcgcctcga cgcccttaat ccgcgctttc
     1261 ccttcgttgt aatatttcag ggtgtgatca tcataatcga tcaagtcact gacatcgtta
     1321 cgatatccgg aaatacgcca gttcacccca gcggttaagc cttcaaacgc gccttcccac
     1381 tgtttgcttt tctccgggtc cagattcgga tttccgtaga agccatacag ttgcccctga
     1441 tttggtgcct tataagatgt cccgtaggaa gcaatgaagc gataaccttc gatgaattcc
     1501 caaccggcgc tggtttgcca ggttccatga cgaccaaact gtgagttatc gtcactgcgt
     1561 gctgcgcctt caaaggtaaa atcgccgact tgttgcagcc cggtcagata gatgccggta
     1621 ttacgttgat catatccatc ctcaacataa cctgtacccg gcgtcgtagt ctgtttctgc
     1681 cagtcgacac ccgcaccaat actaccgtga ccaacgatga cattgtttgc ccactggacg
     1741 gtgtattgct tcatctcatc gagcgtcgcc gacgaatcat aacgaccata atggggatcg
     1801 tagttgtaat ctttgctatg gctatagctg gtaatgagtt gtgatttaat cagttcgccg
     1861 ttatagcgca gcccggcgtc ccaactttgg ctatagagtt tacgggtatc gagcaacggt
     1921 gaaccgggag aataatacgc gtcataattg gtacggttat catagccata gccgcgcaca
     1981 aagccgctcc aggcatcagt aaagttatgc tccagcgcgc cataaagcgt tttacttaaa
     2041 aaaccatcgt tatctgtctg cgcttgcgtt ccggtattac cataggcaac aacatcataa
     2101 ccatgagtat gggcataatc gcccaacagc gttacccgtg tcttatcccc cagttgttgc
     2161 tgcgtagaga catcatagtt ctgataacta ttgcttcccc accctgctga aatttccgtt
     2221 ccgggttcat cgcgcgtcgt gatgatattc accaccccgc ctattgcatc ggaaccataa
     2281 acagcggagc gcggcccacg gatatattca acacgctgga caagcgcaat agggaactgg
     2341 ctaaggtcgg cagaaccact cacccccgcc agattcaggc gtacgccatc aattaacacc
     2401 aacacatgac tggcatttgt accgcgaata aaaatagatg agagctgacc tgaaccgccg
     2461 ttttgggtga tatcgacgcc cggaagacgg cgcagcacat cattgaccga ggtcgactgc
     2521 cagcggtcga tatcctgacg ggtcacaacg gtggttggtg caagcacagt gctgcgcggc
     2581 tgttcaaaac ggttagcagt aacgacgaga gtatccgggc tggtatcctg tgcccaagcg
     2641 gaaaatgccg tgacggaaca cgccgtcagc agcgaagctt ttttaatcat tgtaaagcat
     2701 ccacaataga agaaggatgc cgcaggtttc atcaatatta cgcgatgatg agaaccagat
     2761 gcgacgttgg ccggcaggtc ttcgggcttg gaggggtatc taagatacta agagatgatg
     2821 acttcccacc ggatggcagt gtccgcataa cgcaatcatc gcacctttcc ttaccgctgc
     2881 gcgtcagctc cagattcgca ctggattccc tattaactca caggaccggc aagtggatgc
     2941 tacaggttgt aacaagttac tgtccagacg tagctcacaa ataggaattc atcaagatct
     3001 ggacatctga tgagcaatcc ctacaatcgc cgcgtacttt aatttttcag gatacatcat
     3061 gacccccgaa caccttccaa cagaacagta tgaagcgcag ttagccgaaa aagtggtacg
     3121 tttgcaaagt atgatggcac cgttttctga cctggttccg gaagtgtttc gctcgccggt
     3181 cagtcattac cggatgcgcg cggagttccg catctggcac gatggcgatg acctgtatca
     3241 catcattttc gatcaacaaa ccaaaagccg catccgcgtg gatagcttcc ccgccgccag
     3301 tgaacttatc aaccagttga tgacggcgat gattgcaggt gtgcgtaata atcccgttct
     3361 gcgccacaag ttgttccaga ttgattacct cactacgctg agtaatcagg cggtggtttc
     3421 cctgctatac cataagaagc tggatgatga gtggcgtcag gaagcggagg ccctgcgcga
     3481 tgcactgcgc gcgcagaatc tgaatgtgca tctgattggt cgggcaacga aaaccaaaat
     3541 cgagctggat caggattaca tcgatgaacg tctgccggtc gcagggaaag agatgatcta
     3601 ccgtcaggta gaaaacagct ttacccagcc gaacgcggcg atgaatattc agatgctgga
     3661 atgggcgctg gacgtaacca aaggctcaaa aggcgattta ctggagctgt actgcggcaa
     3721 cggtaacttt tcattagcgc tggcgcgcaa ttttgatcgg gtattagcca ccgaaatcgc
     3781 taagccgtcg gttgctgctg cgcaatacaa catcgcagct aaccatattg ataacgtaca
     3841 aattattcgt atggcggcag aagaatttac tcaggcgatg aatggtgtgc gcgagtttaa
     3901 ccgcctgcaa gggatcgact taaagagtta tcagtgcgaa accatttttg tcgacccacc
     3961 gcgcagcggt ctggacagtg aaaccgagaa aatggtgcag gcgtatccgc gtattttgta
     4021 catctcctgt aacccggaaa cgttatgcaa gaatctggaa acattaagcc agacgcacaa
     4081 ggtcgaacgt ctggctctgt ttgatcagtt cccctacacg caccatatgg agtgcggcgt
     4141 attactgacc gcgaagtaaa acccatgccg gatgcgccag catccggcat aataccgatt
     4201 actcagcaac ttcctgctta cggttacgca tcttcgcgcc aatccagaac accataatga
     4261 cggagagtac tgccgggaag aagttagagc cgatatccgg atattccgca cgaactaccg
     4321 tgctgtacag cagtacgccg agaataaaac aggcagctgc cagacccggc aagcctaccg
     4381 gcatagtgcg attaagataa cgttgatgca ggcagtaaac cgtcagcacc agggaaataa
     4441 tcgggaatac agaaaatggc acaatggagc taaacagcgc tgcgaaagta ccattaatcg
     4501 ataagccagc aactaacgcc agcagcagcg tacctctatc ttgatttgct tgtttcatta
     4561 ctcgtccttc acatttcccg gaataattgc ggttttctct tgttcacggc gataccagta
     4621 ataagcccct ttcgaaatca ttcgcagttg cagtaccagt cgctcttcta attgccgacg
     4681 ttgttcgacg ccgacgtcca acgcctcggc acccgcactg aagacaattg tcaccattgc
     4741 ttcggcttgc gcttcagtaa acgcacgcgg catatggttt tcgagttcca gatagtccgc
     4801 aagttccgca atgaagtgct gaatttcacg cgcaacggcg gcacgaaacg cagcggaggt
     4861 gccggagcgt tcccgcaata ataaccggaa ggcgttagga ttattaccga tgaactccat
     4921 aaatgtggag accgaggtgc ggatcacact cccgcctttg gcgatacgct gacgcgcctg
     4981 gcgcatgagt tggcgtagca ttaaaccgct ctcatcaacc atggtcagac ccagttcgtc
     5041 tacgtcgcgg aaatgccgat aaaaagaggt gggagcaatg cccgcttcac gcgccacttc
     5101 acgcaaactc aggctggcga agctgcgttc agcacttaat tggctaaatg cggcttccac
     5161 cagcgaacgg cgggtttttt ctttttgttg cgctcttacg cccatcacga tgtctgaatc
     5221 cttgccaaaa gtactgctgg cactatacca gagaatgaac atactggatt gctccagtat
     5281 tgtgaatgaa cggtaacgca ataaaacata tttactgcaa ttctttggcc ggttctttta
     5341 cgtacagcgg aaacctgccg cttaaacgga gagtatcgtc gataaaaatc caataaaacg
     5401 tcagggcaaa agtaagaaac agacaaagca aaggccgctc aggatatagc cagataaatg
     5461 acggggatca attggcttac ccgcgataaa atgttaccat tctgttgctt ttatgtataa
     5521 gaacaggtaa accctaccat gccacattcc tacgattacg atgccatagt aataggttcc
     5581 ggccccggcg gcgaaggcgc tgcaatgggc ctggttaagc aaggtgcgcg cgtcgcagtt
     5641 atcgagcgtt atcaaaatgt tggcggcggt tgcacccact ggggcaccat cccgtcgaaa
     5701 gctctccgtc acgccgtcag ccgcattata gaattcaatc aaaacccact ttacagcgac
     5761 cattcccgac tgctccgctc ttcttttgcc gatatcctta accatgccga taacgtgatt
     5821 aatcaacaaa cgcgcatgcg tcagggattt tacgaacgta atcactgtga aatattgcag
     5881 ggaaacgctc gctttgttga cgagcatacg ttggcgctgg attgcccgga cggcagcgtt
     5941 gaaacactaa ccgctgaaaa atttgttatt gcctgcggct ctcgtccata tcatccaaca
     6001 gatgttgatt tcacccatcc acgcatttac gacagcgact caattcttag catgcaccac
     6061 gaaccgcgcc atgtacttat ctatggtgct ggagtgatcg gctgtgaata tgcgtcgatc
     6121 ttccgcggta tggatgtaaa agtggatctg atcaacaccc gcgatcgcct gctggcattt
     6181 ctcgatcaag agatgtcaga ttctctctcc tatcacttct ggaacagtgg cgtagtgatt
     6241 cgtcacaacg aagagtacga gaagatcgaa ggctgtgacg atggtgtgat catgcatttg
     6301 aagtcgggta aaaaactgaa agctgactgc ctgctctatg ccaacggtcg caccggtaat
     6361 accgattcgc tggcgttaca gaacattggg ctggaaactg acagtcgcgg acagctgaag
     6421 gtcaacagca tgtatcagac cgcacagccg cacgtttacg cggtgggcga cgtgattggt
     6481 tatccgagcc tggcatcagc ggcctatgac caggggcgca ttgccgcgca ggcgctggta
     6541 aaaggcgaag ccaccgcgca tctgattgaa gatatcccta ccggcattta caccatcccg
     6601 gaaatcagct ctgtgggcaa aaccgaacag cagctgaccg cgatgaaagt gccatatgaa
     6661 gtgggccgcg cccagtttaa acatctggca cgcgcacaaa tcgtcggcat gaacgtgggc
     6721 acgctgaaaa ttttgttcca tcgggaaaca aaagagattc tgggtattca ctgctttggc
     6781 gagcgcgctg ccgaaattat tcatatcggt caggcgatta tggaacagaa aggtggcggc
     6841 aacactattg agtacttcgt caacaccacc tttaactacc cgaccatggc ggaagcctat
     6901 cgggtagctg cgttaaacgg tttaaaccgc ctgttttaac actttatcga aatggccatc
     6961 cattcttgct cggatggcct ctgccagctg ctcatagcgg ctgcgcagcg gtgagccagg
     7021 acgataaacc aggccaatag tgcggcgtgg ttccggctta atgcacggca gataaacaac
     7081 cccatcgcgt ttgcgctccg gcggcacagc cagcgctggc agtaaagtga tcccgctacc
     7141 tgccgccacc atgttgcgca gcgtctccag gctggtcgcg cggaagtgtg tatcttcatc
     7201 cgccccggct tcaaaacaga aacccattgc ctgatcgcgc aaacagtgac catcttccag
     7261 catcagcagt ttttcccctg ccagatcggc catcggtacg cattcgcggt tcgcccacgg
     7321 gtgatcttca tagatagcca gcaacattgg ctcatcaaac aacggcactt caatgaatgc
     7381 ttcgctctct ttcaccagcg cgaggatcac gcaatcgagt ttgccgctgt ccagttgcgc
     7441 cagtaactgg tgggtctgtg cttcatgcag atacatttcc agctttggaa aggtctggtg
     7501 cagcataggg ataatatgcg gtagcaggta cggtccaact gtgggaatca aaccaatgtg
     7561 cagcggcccg gacatcgtct cgccctgctg gcttgccatc tctttaagga ctttcacctc
     7621 acgcagtacg gtacgcgcct gatctaccag cagcattccc gcctgggtga acaacacttt
     7681 acggctggtc cgctccagca acatcacgcc caactcatct tccagcttac gaatttgccc
     7741 gctaagcgtc ggctggctaa cgtggcagga atctgccgca cgccgaaaat ggcggtgttc
     7801 ggccaatgcc accaggtact caagatcacg aatattcatt atccatcctc cattgccacg
     7861 atagttcatg gcgataggta gaatagcaat gaacgattat ccctatcaag cattctgact
     7921 gataattgct cacagaaacg gagcggcacc tcttttaacc cttgaagtca ctgcccgttt
     7981 cgagagtttc tcaactcgaa taactaaagc caacgtgaac ttttgcggat ctccaggatc
     8041 cgcttttttt gccataaaaa agcccggcga taagccaggc tcaaatttat acatataaat
     8101 gttcttaccc caaccgcgcc tgcgcaaaag caatcgcctg cgccacctgc tgcggtgaga
     8161 cgccgccttt tgccgcacgc ttgtcgaggc acgattgcag cgacagaatc ggatagacat
     8221 cttcgtcaat cacctgactg aatttctgca actcactgag cggcagatct tccagcggtt
     8281 tgccctgacg aatggcttcc accaccgctt caccaacaat atggtgcgcc tcgcggaacg
     8341 gtacgccttt cgccaccaga taatccgcca gttcggtggc gttggcgtaa ccctgctgag
     8401 ccgctacctg gcaacgtgga cgtttcacct gaatgccgtc cagcaccagc gccgccatat
     8461 gcaggcagtc cagccaggta tcgagcgcgt cgaacagacc ttctttgtct tcctgcatat
     8521 ctttgttgta agccagcggc aaacctttca gcgtcatcat catgccggtt aacgcgccct
     8581 gcacccgacc gcatttaccg cgaatcagct ccagcgcatc cgggtttttc ttctgcggca
     8641 ttaatgatga accggaagtc acgcggtccg aaagctccac aaaccccgct tcgccggtgt
     8701 taaagaaaat cagatcttca gcaaaacgcg acagatgcac catgccgata gcggcagcag
     8761 aaagcagttc caacacatgg tcacggtcag aaacgctgtc cagactgtta cgggtcgccg
     8821 aagcaaagcc cagccagcct gctaactgtt cacggtcgat ttcataggcc gttcccgcca
     8881 gcgcgccaca gcctagcggg ctgacatcca gacgcttaag tgcatcctgc aagcggcttt
     8941 catcacgcgc cagcatctca acataggcca ggcaccagtg cgcgaacgtc accggctggg
     9001 cgcgttgcag gtgagtgtaa cctggcatca ccgcgtcctg attgttttgt gcggtttcca
     9061 ccagcgccga ttgcagctgc cggttagccg tcagcaactc gccaacggta tctttgcacc
     9121 acagtttcag gtctgtcgct acctgatcat tacggctacg cccggtatgc agctttttgc
     9181 ctaactggcc cactttgtcg atcagtttgc cttccaccca gctatggata tcttcggcgt
     9241 cgctttcaag gatttgttgt ggcctggcgc gaacatcttc cagcaacacg ttcagcgcct
     9301 cttccagttg cgcctgctct tctgcggtta acacgccgac cgttaccagg gctttggacc
     9361 aggccacaga gccaacaata tcctgctccg ccagacggta atcaaagcgc agtgagtcgt
     9421 tgaattgttt gaaccgttga tctgctgcct gggtaaaacg cccgccccaa agtgccataa
     9481 ctctgtttcc ttattttgaa attcaatgcc ggaaagctgc gcctccggcc aacaaaactt
     9541 aagctaaaat ccgcgtaccc atcggcatac cgttaaacag tgccggaagc tgttccgcat
     9601 gacgccagga ggcgatatct accggacggc ccaacgtgcg cgcagcatcc agcgccgcgt
     9661 tcactttcac tatcatgccg tcagtaataa tgccctgctc aatcagttgt tctgctttcg
     9721 cggcggtcat ttcggcaatg cgttgccctt tgccgtcgag aatgccgctg acatcagaga
     9781 gcaaaatcag atccgcgccc agcgttgccg ccagcgccgt cgccgcctgg tcggcattga
     9841 cgttcatcag ttgcccttcg tctgttacgc caatggagct gaccaccggc agatagccgt
     9901 tctccagcaa ggtgttgata agcttaggcg aacctggctg cgccagtcca acatgaccta
     9961 actcttcatc aagctgggtc actttgacgc tgtcgccgtc accgagaaac aaacctacgg
    10021 ccgcaatctg atgtttcttc gcccatgcca gcagggtttt atttgccgtt cctgccagtg
    10081 ctccggtgat aatgtctatc tgatccgcag gcgtcacccg caggccattt ttctttttca
    10141 ccggcagatt cagccctttc atcagctcat ccaccacgca accgccgccg tgcacaatca
    10201 ccagcggacg ctgatgtgac tcacgataat tcaccagtgc gctaaacaga cgttccagcg
    10261 cctcttcact atccagcagt acgccgccca gtttgataat taatggattc atcattgcac
    10321 ccttaaataa gagactgcgt ttccgcatag ccgaaacgaa tattggcgca ctgtaccgct
    10381 tgtgccgccg cgcctttcag taagttgtct tcggtcgcca caataatcag atgctcgccc
    10441 tgaacggcaa acccgatatc gcaaaatggc agcccaacga catttttcag cgccggaacg
    10501 cctttgtcat acagccgcac cagcggttta tgggcatacg cctgctgtaa cacttgcgcg
    10561 acttgcgcct gggtcacacc cgatttcagg cggcaggtaa tggtttcgag aatgccgcgc
    10621 gggaaattgc ccagatgtgg ggtgaagata acgtcagcac cgaggtgtgt ggcgatctct
    10681 ggttgatggc gatgagtaaa gacgccatac ggttgcaggc taacttcaca aaaactattt
    10741 gaaatcgctg ccttacgtcc tgcaccgctc acgccgctgg tggcgttgat caccggccac
    10801 tgattgaggt caagaagatc ggcatcaatc aacggtttca gcgccagctg tgccgccgtc
    10861 ggataacagc ctggcaccgc aatcaaattc gcttctttta atttattacc gcaccactcc
    10921 gccagaccgt aggctgcctg ttccagcagt tccgggtatt gatgggtaaa gccgtaatat
    10981 ttttcataga aggtggcgtc gttaacacga aacgcgccgg aaaggtcgaa caccacgcag
    11041 cccgcttcaa gaaactgcgg cgctaaatcg tggctaactt catgggcggt ggcgagaaac
    11101 actacgtcca cccctgggct aaactcgctg atatccgaca tcggctgcaa cggcagctca
    11161 acgatgcctt ttagctgcgg atgcaaatcg gagattaact ttcccgcatc attgctttgc
    11221 gctgaaacag tcaaagcggt tatgttcata tgcggatggc gatttacata ggtcactagc
    11281 tctgcgccag cgtagccgct ggcacccaca atcagcgtat tcaacatcgg ggctattcac
    11341 cttcttatgt ctggttgcca ggttaaacgt aaaacattca ccttacggct ggtgggtttt
    11401 attacgctca atgttagtgt gtttttattc ataaatactg catgaatatt gatactatca
    11461 tgaccagagg tgtgtcaaca atgaaaaaca aattaccgcc atttatcgag atttaccgtg
    11521 ctctgattgc cacaccttca ataagcgcca cggaagaggc actcgatcaa agcaatgcag
    11581 atttaatcac tctgctggcg gactggttta aagatttagg cttcaatgtg gaagtgcagc
    11641 ctgtgccagg aactcgcaac aaattcaata tgctggcaag tatcggacag ggggctggcg
    11701 gcttgttgct ggcggggcat accgatacgg tgccatttga tgacggtcgc tggacgcgcg
    11761 atccgtttac actgacggag catgacggca agctttacgg cttaggcacc gccgacatga
    11821 aaggcttttt tgcgtttatc cttgatacgc tacgcgatgt cgacgtcacg aaactgaaaa
    11881 aaccgctcta cattctggcg actgctgatg aagaaaccag tatggccgga gcgcgttatt
    11941 ttgccgaaac taccgccctg cgcccggatt gcgccatcat tggcgaaccg acgtcactac
    12001 aaccggtacg cgcacataaa ggtcatatct ctaacgccat ccgtattcag ggccagtcgg
    12061 ggcactccag cgatccagca cgcggagtta acgctatcga actaatgcac gacgccatcg
    12121 ggcatatttt gcaattgcgc gataacctga aagaacgtta tcactacgaa gcatttaccg
    12181 tgccataccc tacgctcaac ctcgggcata ttcacggtgg cgacgcttct aaccgtattt
    12241 gcgcttgctg tgagttgcat atggatattc gtccgctgcc tggcatgaca ctcaatgaac
    12301 ttaatggttt gctcaacgat gcattggctc cggtgagcga acgctggccg ggtcgtctga
    12361 cggtcgacga gctgcatccg ccgatccctg gctatgaatg cccaccgaat catcaactgg
    12421 ttgaagtggt tgagaaattg ctcggagcaa aaaccgaagt ggtgaattac tgtaccgaag
    12481 cgccgtttat tcaaacgtta tgcccgacgc tggtgctcgg tccgggttcc atcaatcaag
    12541 ctcatcagcc tgatgaatat ctggagacgc ggtttattaa acccacccgt gagttgatta
    12601 tccaggtaat tcaccatttt tgctggcatt aaaacgtagg ccggataagg cgctcgcgcc
    12661 gcatccggca ccgttgccaa actccggtgc cgcaataatg tcggatgcga tgcttgcgca
    12721 tcttatccga cctacacctt tggtgttatg tatggcgatt ttttaacatt tccataagtt
    12781 acgcttattt aaagcgtcgt gaatttaatg gcgtaaattc ctgctattta ttcgtttgct
    12841 gaagcgattt cgcagcattt gacgtcaccg cttttacgtg gctttataaa agacgacgaa
    12901 aagcaaagcc cgagcatatt cgcgccaatg cgacgtgaag gatacagggc tatcaaacga
    12961 taagatgggg tgtctggggt aatatgaacg aacaatattc cgcattgcgt agtaatgtca
    13021 gtatgctcgg caaagtgctg ggagaaacca tcaaggatgc gttgggagaa cacattcttg
    13081 aacgcgtaga aactatccgt aagttgtcga aatcttcacg cgctggcaat gatgctaacc
    13141 gccaggagtt gctcaccacc ttacaaaatt tgtcgaacga cgagctgctg cccgttgcgc
    13201 gtgcgtttag tcagttcctg aacctggcca acaccgccga gcaataccac agcatttcgc
    13261 cgaaaggcga agctgccagc aacccggaag tgatcgcccg caccctgcgc aaactgaaaa
    13321 accagccgga actgagcgaa gaaaccatca aaaaagcagt ggaatcgctg tcgctggaac
    13381 tggtcctcac ggctcaccca accgaaatta cccgtcgtac actgatccac aaaatggtgg
    13441 aagtgaacgc ctgcttaaaa cagctcgata acaaagatat cgctgactac gaacacaacc
    13501 agctgatgcg tcgcctgcgc cagttaatcg cccagtcatg gcatactgat gaaatccgta
    13561 agctgcgtcc aagcccggta gatgaagcca aatggggctt tgccgtagta gaaaacagcc
    13621 tgtggcaagg cgtaccgaat tacctgcgcg aactgaacga acaactggaa gagaacctcg
    13681 gctacaaact acccgtcgaa tttgttccgg tccgttttac ttcgtggatg ggcggtgacc
    13741 gcgacggcaa cccgaacgtc actgccgata tcacccgcca cgtcctgcta ctcagtcgct
    13801 ggaaagccac cgatctgttc ctgaaagata ttcaggttct ggtttctgaa ctgtcgatgg
    13861 ttgaagcgac ccctgaactg ctggcgctgg ttggcgaaga aggtgccgca gaaccgtatc
    13921 gctatctgat gaaaaacctg cgttctcgcc tgatggcgac acaggcatgg ctggaagcgc
    13981 gcctgaaagg cgaagaactg ccaaaaccag aagggctgct gacacaaaac gaagaactgt
    14041 gggaaccgct ctacgcttgc taccagtcac ttcaggcgtg tggcatgggt attatcgcca
    14101 acggcgatct gctcgacacc ctgcgccgcg tgaaatgttt cggcgtaccg ctggtccgta
    14161 ttgatatccg tcaggagagc acccgccata ccgaagcgct gggcgagctg acccgctacc
    14221 tcggtatcgg cgactacgaa agctggtcag aggccgacaa acaggcgttc ctgatccgcg
    14281 aactgaactc caaacgtccg cttctgccgc gcaactggca accaagcgcc gaaacgcgcg
    14341 aagtgctcga tacctgccag gtgattgccg aagcgccgca aggctccatt gccgcctacg
    14401 tgatctcgat ggcgaaaacg ccgtccgacg tactggctgt ccacctgctg ctgaaagaag
    14461 cgggtatcgg gtttgcgatg ccggttgctc cgctgtttga aaccctcgaa gacctgaaca
    14521 acgccaacga tgtcatgacc cagctgctca atatcgactg gtatcgcggc ctgattcagg
    14581 gcaaacagat ggtgatgatt ggctattccg actcagcaaa agatgcgggc gtgatggcag
    14641 cgtcctgggc gcaatatcag gcacaggatg cattaatcaa aacctgcgaa aaagcgggta
    14701 ttgagctgac gttgttccac ggtcgcggcg gttccattgg tcgcggcggc gcacctgccc
    14761 atgcggcact gctgtcacaa ccgccaggaa gcctgaaagg cggcctgcgc gtaaccgaac
    14821 agggcgagat gatccgcttt aaatatggtc tgccagaaat caccgtcagc agcctgtcgc
    14881 tttataccgg ggcgattctg gaagccaacc tgctgccacc gccggagccg aaagagagct
    14941 ggcgtcgcat tatggatgaa ctgtcagtca tctcctgcga tgtctaccgc ggctacgtac
    15001 gtgaaaacaa agattttgtg ccttacttcc gctccgctac gccggaacaa gaactgggca
    15061 aactgccgtt gggttcacgt ccggcgaaac gtcgcccaac cggcggcgtc gagtcactac
    15121 gcgccattcc gtggatcttc gcctggacgc aaaaccgtct gatgctcccc gcctggctgg
    15181 gtgcaggtac ggcgctgcaa aaagtggtcg aagacggcaa acagagcgag ctggaggcta
    15241 tgtgccgcga ttggccattc ttctcgacgc gtctcggcat gctggagatg gtcttcgcca
    15301 aagcagacct gtggctggcg gaatactatg accaacgcct ggtagacaaa gcactgtggc
    15361 cgttaggtaa agagttacgc aacctgcaag aagaagacat caaagtggtg ctggcgattg
    15421 ccaacgattc ccatctgatg gccgatctgc cgtggattgc agagtctatt cagctacgga
    15481 atatttacac cgacccgctg aacgtattgc aggccgagtt gctgcaccgc tcccgccagg
    15541 cagaaaaaga aggccaggaa ccggatcctc gcgtcgaaca agcgttaatg gtcactattg
    15601 ccgggattgc ggcaggtatg cgtaataccg gctaatcttc ctcttctgca aaccctcgtg
    15661 cttttgcgcg agggttttct gaaatacttc tgttctaaca ccctcgtttt caatatattt
    15721 ctgtctgcat tttattcaaa ttctgaatat accttcagat atccttaagg aattgtcgtt
    15781 acattcggcg atattttttt caagacaggt tcttactatg cattccacag aagtccaggc
    15841 taaacccctt tttagctgga aagccctggg ttgggcactg ctctactttt ggtttttctc
    15901 tactctgcta caggccatta tttacatcag tggttatagt ggcaccaacg gcattcgcga
    15961 ctcgctgtta ttcagctcgc tgtggttgat cccggtattc ctctttccga agcggattaa
    16021 aattattgcc gcagtaatcg gcgtggtgct atgggcggcc tctctggcgg cgctatgcta
    16081 ctacgtcatc tacgggcagg agttctcgca gagcgttctg tttgtgatgt tcgaaaccaa
    16141 caccaacgaa gccagcgagt atttaagcca gtatttcagc ctgaaaattg tgcttatcgc
    16201 gctggcctat acggcggtgg cagttctgct gtggacacgc ctgcgcccgg tctatattcc
    16261 aaagccgtgg cgttatgttg tctcttttgc cctgctttat ggcttgattc tgcatccgat
    16321 cgccatgaat acgtttatca aaaacaagcc gtttgagaaa acgttggata acctggcctc
    16381 gcgtatggag cctgccgcac cgtggcaatt cctgaccggc tattatcagt atcgtcagca
    16441 actaaactcg ctaacaaagt tactgaatga aaataatgcc ttgccgccac tggctaattt
    16501 caaagatgaa tcgggtaacg aaccgcgcac tttagtgctg gtgattggcg agtcgaccca
    16561 gcgcggacgc atgagtctgt acggttatcc gcgtgaaacc acgccggagc tggatgcgct
    16621 gcataaaacc gatccgaatc tgaccgtgtt taataacgta gttacgtctc gtccgtacac
    16681 cattgaaatc ctgcaacagg cgctgacctt tgccaatgaa aagaacccgg atctgtatct
    16741 gacgcagccg tcgctgatga acatgatgaa acaggcgggt tataaaacct tctggatcac
    16801 caaccagcag acgatgaccg cccgcaatac catgctgacg gtattttcgc gccagaccga
    16861 caagcagtac tacatgaacc agcaacgtac gcagagtgcg cgtgaatacg acaccaacgt
    16921 gctgaagccg ttccaggatg tgctgaatga ccctgcgccg aagaaactga tcatcgttca
    16981 tctgctgggt acgcatatca aatacaaata ccgctacccg gaaaatcagg gcaagtttga
    17041 tggcaatacc gatcatgttc cgccaggatt aagcgcagaa gagctggaat catataacga
    17101 ttatgacaac gctaacctgt ataacgatca tgtggttgcc agcctgatta aagactttaa
    17161 agcggcagac ccgaacggat tcctggttta cttctctgac cacggtgaag aggtttacga
    17221 cacgccgccg cacaaaactc aggggcgtaa cgaagacaac ccgacgcgcc acatgtacac
    17281 cattccgttc ctgctgtgga cgtcggaaaa atggcaagcg actcatcccc gtgatttctc
    17341 acaggatgtc gatcgtaaat acagccttgc ggaactgatc cacacctggt cagatttagc
    17401 gggcttatct tacgacggtt acgatccaac ccgttcagtg gtgaacccgc agttcaaaga
    17461 aactacccgc tggattggta acccgtacaa gaaaaacgcg ctgatcgatt acgacacact
    17521 gccgtatggc gatcaggtgg gtaatcagta attatcagcc aggcgctatt tgccggatgc
    17581 gacgcttgac gcgtcttatc cggcctacag atcgcggcgg taggctggat aaggcgttta
    17641 cgccacatcc ggcaatacaa ctgccgcaat gtttacatcg aactcccctc aggccacact
    17701 tttcaggtgg ccttttttaa taatgccagt tactgttttc ccgtctacat acctaaggga
    17761 ataacatgta tcacgacgtc agctatctgc tctcccgcct gatcaacggc ccgctgtccc
    17821 tgcgccagat ttactttgcc agcagtaacg gcccggtgcc cgatcttgcg tatcaggtcg
    17881 attttccacg gctggaaatt gtgctggaag gtgagtttgt tgataccggc gctggagcaa
    17941 cgttagttcc cggcgatgtg ctgtacgttg ccgctggtgg ctggaatttt ccacaatggc
    18001 aagcccccgc taccaccttt agcgtgctgt ttggcaaaca gcaactcggc ttcagcgtcg
    18061 tgcaatggga tggcaaacaa tatcaaaatc tggcgaagca acacgtcgcc cgacgcggcc
    18121 cacgtatagg ttcttttctg ctacaaacgc tcaatgaaat gcaaatgcag ccgcaggagc
    18181 agcaaacggc aaggcttatc gtagccagcc tgcttagcca ctgccgcgat ttgctcggca
    18241 gccaaatcca gaccgcctca cgcagccagg cactatttga agctattcgc gattatatcg
    18301 acgaacgcta tgcctccgcg cttacccgcg aatctgtcgc acaggcattc tatatttcac
    18361 caaactatct ctcgcacctg tttcaaaaaa cgggggccat tggttttaac gaatacctga
    18421 atcacacgcg actggagcac gctaagacgt tattgaaagg ctatgacctg aaggtaaaag
    18481 aagtggcgca cgcctgcggc tttgtcgaca gcaactactt ctgccgtctg tttcgtaaaa
    18541 acaccgaacg ctcgccgtcg gaatatcgcc ggcagtacca cagccaactg acagaaaaac
    18601 cgactactcc agaataagat gggtttgctg cggcgcagaa agcactttgc gcaccgcgct
    18661 cattacccgc tgcggctcac gcaggaatgc gtagatgctg cattgcacat agcggcaatg
    18721 ttcaaatcgc tcggcaccgg caagctcgat atccgtaatc aacagagcaa catccgcccg
    18781 acggatatcc tcgtccgcta aacgattctc cgttcccagc gcgccctgag tttcaatgct
    18841 gacgccccac ttctctaact ggcacaactt ttccagccgt tccgccgcca tataagtatg
    18901 cgccacgccg cttacgcagg cggttactgc caccaggtat gccattttaa cctcccacgg
    18961 taacctgaaa tccggcccgt tctgccattt cgcgcatcgt tgtcacatcg gctgacgacg
    19021 gcgcaggcac ctctttcatc gaccatgttt tccccagcag gcggtatttc ggttcgccgt
    19081 actgatgaaa cggtaacaga tggatctgcc tgattttcag cgggatcagt acatccagcg
    19141 cctgctgcat attctcccgg ctgagcgtga aaccagggat caacggtaaa cgcgggatca
    19201 cgttgacacc ctcactcacc agcaaacgca gattctccag cacgcgtggc aggttcatct
    19261 tcaccacatc ccgcgcctga gtcgcgtcca taatttttaa atcgaacagc acttcatcgc
    19321 acaatttcgc cagtggtaat agcttgctgg ctggcgcgtc tctggcagtt tcaatggcgc
    19381 atgacacgcc ccacaaccgc agccgctgta aaaaacgggt agcaaactcc gcctgcatta
    19441 acacttcgcc gccagaaagc gtgacgccgc cgccggacgt gcgaaagaaa atgtcatctt
    19501 tcatcacttc ccgttccagc gcgtcaaggc tgatatcgcg gccaatccgt tcaaacgccc
    19561 cggaggggca ttcatccgca tcacgcaaac atttcgcaca gtgcagacat ttcgcctctc
    19621 tgcgtaccgt ctggattttg ccggagatcg actccggatt agcacaccac gggcaaagat
    19681 gcggacagcc tttaaaaaag accaccgtac gaatgccctc gccgtcattc agtgaataac
    19741 gctgaatgtt gaaaatgcgc gccacatcat cgcggcgcgt ttccacaacg ttacagctga
    19801 tgcgctgtcc ggcggatgat gtcatcctgg atctccttcg acagttcgac aaagaaggcg
    19861 ctgtatccgg caacgcgcac caccagcccg gcataatctt gtgggcgctg ttgcgcttcc
    19921 cgcaacgtgt cggcgttcac cacgttaaac tgaatatgct gcaacttaag ctgggtaaac
    19981 gcccgtaaga agtcggccag tttgcgtaat cccgcttcac cttccagggt cgccggagtg
    20041 aatttcacgt tcagcaacgt accgttagac agcagcgtat tatcgagctt actgactgac
    20101 ttcagtaccg ctgttggccc ttgtgcgtcc tggcccagca taggtgacaa gccgccgtct
    20161 gccagttgtt ccccggcaaa acgaccgtct ggcgtcgcgc caaccaccga tcccaacggg
    20221 acgtgagcag aaacggtata cgatcccggc gtgaagtaac cgccgcgcgg gttctggtat
    20281 ttttccactt ctttgcagta gtggcgtaac cgttcggcgc tgatgttatc cacctcgtcg
    20341 atatcgttac cgtatttctc gaagcggtta attaagcgag cacggacttt ttcgccttct
    20401 ggcgttgcaa agttggcttt taataccgac agcaattcgt caaaacttaa acgctgttgc
    20461 tcaaaaacca tccctttcaa cgcatggaga gaatcgctca ggttggcgat accgatcccc
    20521 tgtacgccgg agaagttata acgcgcgccg ccatcggtaa tatcgcgacc tttttccaga
    20581 caatcgctga taaaagacga gagcagcggt acaggtgccc agtcgcgatg gccgatatca
    20641 caaatattgc tgccctcaac catcagggtg atgtagtggc tgatcttggc gcggatctgc
    20701 tccagtaaac cttcataagt cagcgcagca ttgccttcat tttcatgcag gcagatttcc
    20761 atcactttca gcagattaaa catcgcgatg tcatgcaagc cgtaggttct gccgggaata
    20821 gataattcca cacagcccac tacggaatag tcgcgcgcat cttccagcga aacgccacgg
    20881 ttgaggaacg ctggtaccac cacttcatcg ttaaagattt gcggaatacc ggtgccgagg
    20941 cgaatggttt cggcggtttt catcaggaac ggcgtgtcga tcaaggcgtt agtgcgcacg
    21001 ccgaggttcg gttgcggtaa ttgcacgctt tgataggcgt caaggcaaag gaacgaaagc
    21061 acgttcaccg cgctacgtcc gttctcggtt aacccgccga gcagtgcggt atagccggtc
    21121 gggaaacctg cgaaataacg cgcgctgttg gtggagcgca acagcacgat gtcgttgcat
    21181 ttcacccata aagattcgag cagttctttc aggaacgccg gatcttcgcc ctgggttaaa
    21241 gatgcctgat agaacggcaa catatactgg tcgaagcgcc ctaaagacag cgaactggcg
    21301 ttggattcgt attgcagaat gatgttcatg taccagaata actggcacgc ctgccagaac
    21361 gtctgcggct tatgttcggc gttatggcga gagatctccg caatagtcag cagttcttcg
    21421 cgacgctgtg catctgtgca gtttgccgcc atcgtttccg ccagttcggc gtaacgcaaa
    21481 atgtgtttct gcgaggcttc cagcagtaac aacgcggcct gataaaagtg attctccggc
    21541 tgttgctgac aatgttgctg catctgtgcc accagctcac ccagaccgtg attcaacagg
    21601 cgtgggtaat caataataat gtgcccctgg cctttatccg tctggttgat gctgaaaatc
    21661 tgcgtgttgg tcgcggcttt tacttcatct gtcatctgcc cgttgatgaa atctttcatc
    21721 gaacgttttt cccagtacgg gaacaactct tcacggtaga tacgtttatc ttcttcgctg
    21781 atagcaaagc ggtcctgcgg acgcgtcggg aattgatcca gctctttcag cagccagtaa
    21841 gggtccattt ccggcgacat aatcccggcg cgcggtttta cggtgcggtt accggcaatc
    21901 agttcttcat cacgaatcga aatttcaaca tgttcaagga tatacgctgt tgctttcgcc
    21961 cggcgcaata tcaccggttc gccttcggtt tgccgatggc tggcggtata aagcagcgcc
    22021 cgctccagcg agatttcacg ggtattggca aacagtgcag ttttgaggcg agagatacga
    22081 ttcgtcatga ggaactcctg tgggaaagcg aacccgccct tgcgagcggg agaagaactt
    22141 aagcggtttg cgccaggtga gcttcaattt tgctcataat ggcgtcagca cgttttactg
    22201 catcgctgat gttgacgcgc acgatggtct tgccagcaaa acgctcttca aacttgatgc
    22261 cgatatcttt ggtcagaatc accatatcag cgctcgccac atcttccgca gtcagttcat
    22321 tttccagacc aattgacccc tgggtttcta ctttcacttc ccagcctttc gctttcgcgg
    22381 cactttccag cgcctctgct gccatatagg tatgggcaac acctgaagga catgcggtta
    22441 ctgcaataat tttcgtcatg gctcgttcct caattaatta atttcaaaat ccaaatccag
    22501 gtcgtcttct ttttcatcag tcgaagaccc atttttacgc gccagacttt tcagcacgtt
    22561 cacacaaaca gccgtcacca ctgctccgac agccactgct gcgatataac ccagcttgcc
    22621 ttcaaccacc ggcagcacaa tcagaccacc ccagcctgca tagcactgcg caccgaccag
    22681 cgccgcagtt actgcaccac aaactgaacc gaccatgatg gaagaaatga cacgcagcgg
    22741 atcggcagcg gcgaaaggaa tcgccccttc agtaacgcca acacagccca tcaccagtgc
    22801 cgctttgccg gtttcgcgct cttctgcgga gaaattttta cgaccaatca gcgtcgccag
    22861 ccccatcccc agcggcggga tacaaatacc cacggcagcg atggccacca cggtataaac
    22921 accctgagca acgcaaatca gcatgaaggc ataggccact ttgttaaccg gaccgcccat
    22981 atcgaacgcc agcatcagac ccatgatcac cgccagcata acaatgctgc cctgctgcat
    23041 cccctgaagc cactgagtca ggctgttggt caacgcccct acaggctcgc ccaagcccca
    23101 catcatgatg cctgcggtaa tcagtgtgcc gacgatagga ataatgaaga taggcatcac
    23161 cgagcgcaga actttatgca ccggaatttt cttcaggtaa tgcaccacga tgccgccgat
    23221 aatcccggcg atcagtgcac caaagaagcc cgcaccaaag ctgttaccaa cccaggcacc
    23281 gatagcgcac ggagccagcg cagaacgctc tgcaatggaa taaccgatgt acgctgcgag
    23341 gaaaggcacc atcagcgtca agcccgcaac gccgatatca aacagttttt tcagatttgg
    23401 atcggctacg gcatccggca ctgcgccttt gccatacaac atgacggaaa ccgccagcaa
    23461 aataccgccc gataccacga agggaatcat gtgtgaaacg cccgtcatta aatgctgacg
    23521 ggtatttttc aggatctgca ccaactcatt cataagctgc tcccgggctt gcgaaagccc
    23581 atgtccatat ttttgatgga ggaaacaata ggcaatcgca ggcggagcaa acagtggata
    23641 aaaaggccat ttactggact tttgtaatat cagtacaaaa atgcgatccg cctcataact
    23701 tgcgataaac cggaagcatg ccgcaaaaca gcgtcttgtg agacgcgtcg caaattgacg
    23761 gttacattac atttgtccag cttttggcag atttgtcaca tggcaggccg ttgccgtgcg
    23821 ctcttatcct gtaaacagaa aaccgtaccg ggagagaggc aatggccctg attgtggaat
    23881 ttatttgtga gctacctaac ggcgtacatg cgcgtccggc aagccacgtt gaaacgctgt
    23941 gtaatacttt ttcatcacaa attgagtggc ataacctgcg cactgaccgc aagggcaacg
    24001 ccaaaagcgc ccttgcgctg attggcaccg atacgctggc gggcgataac tgccagttac
    24061 tgatttccgg ggccgacgaa caggaagcgc accagcgttt aagccaatgg ctgcgcgatg
    24121 aattccccca ctgcgatgcg ccgctggcgg aagttaaatc tgacgaactg gaaccactgc
    24181 cggtttcact gaccaatctg aatccgcaaa ttatccgcgc ccgcaccgtg tgcagcggta
    24241 gtgcaggcgg cattctgacg ccgatctctt ctttagatct caatgcgctg ggtaatcttc
    24301 ccgcagccaa aggcgttgac gccgagcaat ccgcactgga aaacggcctg acgctggtac
    24361 tgaaaaacat tgagtttcgt ctgctggata gcgacggtgc caccagcgcg attctggaag
    24421 cgcaccgatc cctggctggc gatacttccc tgcgcgaaca tttactggca ggcgtcagcg
    24481 ccggattaag ctgcgccgaa gcgattgtca ccagtgcgaa tcacttttgc gaagagttcg
    24541 cccgttccag cagcagctac ctgcaagaac gtgccctgga cgtacgcgac gtctgcttcc
    24601 agttactcca gcaaatctac ggtgagcaac gcttcccggc accgggcaaa ctgacgcagc
    24661 ccgccatttg tatggctgat gaactgactc ccagccagtt cctcgaactg gataaaaatc
    24721 acctcaaagg attgttactg aaaagcggcg gcaccacctc acatacggtg atccttgccc
    24781 gttcgttcaa cattcctacg ctggttggtg tggatattga tgccctcact ccgtggcagc
    24841 atcaaacgat ttatatcgac ggcaacgctg gggcgattgt ggttgagcca ggagaagccg
    24901 tagctcgtta ttatcagcaa gaagcccgcg tacaggacgc cctgcgtgag caacagcgtg
    24961 tctggctgac ccaacaagcc cgtaccgctg acggtatccg cattgaaatt gccgctaaca
    25021 tcgctcactc cgtggaagcg caggccgcat tcggcaatgg tgcggaaggc gttggtttgt
    25081 tccgcactga aatgctctat atggatcgca ccagtgcacc gggcgaaagc gagctgtaca
    25141 acattttttg tcaggcgctg gagtctgcca acggacgcag cattattgtg cgcactatgg
    25201 acattggcgg cgacaaaccc gttgattatc taaacattcc cgcagaggca aacccgttcc
    25261 tcggttatcg cgccgtgcgt atttatgaag aatacgcgtc gttatttacc acacaactac
    25321 gctcgatcct gcgcgcctcc gctcacggca gcctgaaaat catgatcccg atgatctcct
    25381 caatggaaga gatcttatgg gtgaaagaaa aactggcaga agccaaacag caactacgta
    25441 acgaacacat tccgtttgat gagaagatcc agctcggcat tatgctggaa gtgccgtcgg
    25501 tgatgttcat catcgatcaa tgctgcgaag agattgattt ctttagtatt ggtagtaatg
    25561 acctgacgca atatctgctg gcagtggatc gcgataacgc taaggttact cgtcactaca
    25621 acagcctgaa tccggcattc ttgcgggcgc tcgattacgc cgtgcaggcg gtgcatcgcc
    25681 agggtaaatg gattggtctg tgcggtgagc tgggagcgaa aggttccgtg ctgccgttgc
    25741 tggtcggctt agggctggat gaactcagca tgagcgcacc atcaattccg gcggcgaaag
    25801 cgcggatggc gcaacttgat agccgtgagt gccgcaagtt gctcaaccag gcaatggcct
    25861 gccgtacttc gctggaagtg gaacacctgc tggcgcaatt ccgcatgacc caacaagacg
    25921 caccgctggt caccgccgag tgcatcacac tggaaagcga ctggcgcagc aaagaagaag
    25981 tgctcaaagg catgaccgat aacctgctgc tggcagggcg ctgccgctat ccgcgtaaac
    26041 tggaagccga cttgtgggcg cgcgaggccg ttttctctac cggtctgggc tttagttttg
    26101 ccattccaca cagcaaatca gaacacattg agcaatccac catcagcgtt gcgcgtctgc
    26161 aagcgccggt gcgctggggt gatgatgaag cgcaattcat catcatgtta accctgaaca
    26221 aacacgctgc gggcgatcag cacatgcgca ttttctcgcg cctcgctcgc cgcatcatgc
    26281 acgaagaatt ccgtaacgcg ctggttaacg ccgcctctgc cgacgctatc gccagcctgc
    26341 tgcaacatga actggaactg taaaaggaaa catcatggaa ctgtatctgg acaccgctaa
    26401 cgtcgcagaa gtcgaacgtc tggcacgcat attccccatt gccggggtga caactaaccc
    26461 gagcattatc gctgccagca aggagtccat atgggaagtg ctgccgcgtc tgcaaaaagc
    26521 gattggtgat gagggcattc tgtttgctca gaccatgagc cgcgacgcgc aggggatggt
    26581 ggaagaagcg aagcgcctgc gcgacgctat tccgggtatt gtggtgaaaa tcccggtgac
    26641 ttccgaaggt ctggcagcaa ttaaaatact gaaaaaagag ggtattacta cgcttggcac
    26701 cgcagtgtac agtgctgcac aagggttact ggccgcgctg gcaggggcaa aatacgttgc
    26761 tccgtatgtt aaccgcgtag atgcccaggg cggagacggc attcgtacgg ttcaggagct
    26821 gcaagcgctg ttagaaatgc acgcgccaga aagcatggtg ctggcagcca gctttaaaac
    26881 gccgcgtcag gcgctggact gtttactggc aggatgtgaa tccatcaccc tgcccttaga
    26941 tgtagcgcaa caaatgctca acacccctgc ggtagagtca gctatagaga agttcgaaca
    27001 cgactggaat gccgcatttg gcactactca tctctaaagg agcaattatg gaccgcatta
    27061 ttcaatcacc gggtaaatac atccagggcg ctgatgtgat taatcgtctg ggcgaatacc
    27121 tgaagccgct ggcagaacgc tggttagtgg tgggtgacaa atttgtttta ggttttgctc
    27181 aatccactgt cgagaaaagc tttaaagatg ctggactggt agtagaaatt gcgccgtttg
    27241 gcggtgaatg ttcgcaaaat gagatcgatc gtctgcgtgg catcgcggag actgcgcagt
    27301 gtggcgcaat tctcggtatc ggtggcggaa aaaccctcga tactgccaaa gcactggcac
    27361 atttcatggg tgttccggta gcgatcgcac cgactatcgc ctctaccgat gcaccgtgca
    27421 gcgcattgtc tgttatctac accgatgagg gtgagtttga ccgctatcta ctgttgccaa
    27481 ataacccgaa tatggtcatt gtcgacacca aaatcgtcgc tggcgctcct gcacgtctgt
    27541 tagcggcggg tatcggcgat gcgctggcaa cctggtttga agcacgcgcc tgttcccgta
    27601 gcggcgctac cactatggcg ggcggcaagt gcacccaggc ggcgctggca ctggcagaac
    27661 tgtgctacaa cactctgctg gaagaaggcg aaaaagcgat gcttgccgcc gaacagcatg
    27721 tagtaacccc tgcgctggaa cgcgtgattg aagcgaacac ctatttaagc ggtgttggtt
    27781 ttgaaagcgg cggactggcg gcggctcatg cggtgcataa cggcctgacc gctatcccgg
    27841 acgcgcatca ctattatcac ggtgaaaaag tggcattcgg tacgctgacg cagctggtac
    27901 tggaaaacgc gccggttgag gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt
    27961 tgccaataac tctcgctcaa ctggatatta aagaagatgt cccggcgaaa atgcgaattg
    28021 tggcagaagc ggcatgtgca gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc
    28081 cagatcaggt ttacgccgct ctgctggtag ccgaccagta cggtcagcgt ttcctgcaag
    28141 agtgggaata acctactcca aactcccggc ttgtccggga gtttgaacgc aaaattgcct
    28201 gatgcgctac gcttatcagg cctacgcaat ctctgcaata tattgaattt gcgtgctttt
    28261 taggccagat aaggcgttca cgccgcatcc ggcactctgt gccagtaatc tgaaactcct
    28321 acgttgttca ggagtttttt atccgcttct ggacattttc tctacagaac aatcgttagc
    28381 ccggaaggtc gaatctgtga ctcttatttt cacggacgaa ccagatgaaa gcttcactgg
    28441 cgctgctcag cctgctaacg gcatttacca gccactcctt aaaatctccc gccgttccac
    28501 caaccgtggt acaaatccag gccaatacca accttgccat cgcagacggt gccagacagc
    28561 aaatcggcag cacgctattt tacgatccgg cgtatatgca gcttacctat cctggcggtg
    28621 atgtcccgca aaaacgcggg gtatgttccg atgtggtgat ccgtgcattg cgcagccaga
    28681 aggtcgattt gcagaaactg gtgcacgaag atatggcgaa gaattttgcc gagtacccgc
    28741 aaaaatggca gttaaagcgc ccggacagca acatcgatca ccgtcgggtg cctaatctgg
    28801 aaacctggtt tacccgccac gataaaacac gccccatcag caagaacccc agtgactatc
    28861 aagcgggcga tattgtctcc tggcgattag ataacgggct ggcacatatt ggggtggttt
    28921 cagatggctt cgcccgcgac ggtacgccgc tggtgataca taacattggc gcaggtgcgc
    28981 aggaagaaga tgtgctgttt agctggcgca tggtggggca ttatcgttat tttgttaaat
    29041 aagcgatcgc cccacatgaa gaaaaaattt agatcctttt tacactaacg gcttcttttt
    29101 tcttacggct caccagcgcc agtgccatca caatcagcac aataccgcta ccttcaacgg
    29161 cccccggatt ctcgccgagc agccaccagg aaaagagtac gccgcaaacg ggaacggcca
    29221 gtgtgcttaa gctggcaata ctggcaggca agtttttcaa tacaaacaac cataagctcc
    29281 acgccagtgc cgtcgccaga atcgcactgt aggccagcgc ccagaacacg gtgggctgcc
    29341 agtcaatttc acgttgcggc accagtaaag cgaccacact catcaccagc gccgcataga
    29401 gcatctgcca ggatgttaac gacaataaat ccacgcgcgg atgacgggca tacagacgtt
    29461 tagcaacaat cgcgctcgcc ccccaactga cgccggagag gattgccagc atggcacttt
    29521 tcatcgaaga gaaatcgagt tgccacggct gcaacaccaa aaataagccg aaagcggcaa
    29581 tcagtatcgc gaaatattgc ccacgtcgca ggcgttcacc gagaaacaac gcggcgaaaa
    29641 tcaccaccca gaacggcatg gtatagctta ggatcgccac cttccccgct ccgccgctga
    29701 ccagcgccca ctgcgccagg ccaaccatcc cgcaggtttg taacagggca atggctaagg
    29761 tgtatttaaa cggtgtcggg cgcattccgc gaccacgtaa taaaaggacg atgaataaaa
    29821 cgagagcgcc gaaaatgcag cgtaaggcgg taaagtcgaa ggcaccgatg tagctggtga
    29881 cttgcttcat gaaaatccag ctataactcc agataagtgt gagcacaacc aggccactga
    29941 ttgccagtgg gttgctcttt cctgcggcag acatgagaga tccagtgtgt agttttcctg
    30001 tggacactat acgccggtaa agaaacttca gcgactgcct gaaaggcagt cgctgaagaa
    30061 ggtcagatta cagcaggtcg aaacggtcga ggttcatcac tttcacccat gccgccacga
    30121 agtctttaac aaacttctcg tgagcatcgc tgctggcgta aacttcagcc accgcacgca
    30181 ggacagagtt agaaccaaat accagatccg cacggctggc tgtgtatttc acttcgccgg
    30241 tttcgcggtc acgtccttcg aacagctctt tcgattcgtc agtcgctttc cactcgtaac
    30301 gcatatccag caagttcacg aagaagtcat tgctcaatac gccaacgcgg tcagtgaaga
    30361 cgccgttttt gctgccatcg aagttggcac ccagtacacg catgccgccc accagcgcag
    30421 tcatttccgg cgcggtcagc gtcagttgct gtgctttgtc gatcagcagt gactcggtgg
    30481 tggaaacgtc cagacgagcg cgatagttac ggaaaccgtc agcaattggc tccagcagct
    30541 caaacatctc aatgtcagtc tgatcctgac gcgcatcaac gcgacccggc gcaaacggta
    30601 catgaatgct caaacctgcg gcgcttgcgg ctttctcaac accaaccaca ccagccagca
    30661 cgatgatatc cgccagcgag gctttaccag actctttctg gattttctcc agaacaggca
    30721 gagcacgaac ggctgcggcg ttcacatccc agtcgcgctg cggcattaat gccagacgcg
    30781 caccgttggc accaccgcgt ttgtcgccac cacggaaggt agaagcagat gcccaggcca
    30841 ccgataccag ctcactaaca gacagaccag aatccgcaat cgcgaatttc agatcgataa
    30901 tgtcctgctc ggtcgggttg tagatcggct gcggcagcgg atcttgccag atcagatctt
    30961 ctttcggcac ttccggcccg atgtagcgag atttcggccc catatccctg tgcgtcagtt
    31021 tgaaccaggc acgggcaaag gcttcgttga acgcctgcgg atcgttgagg aaacgacgag
    31081 agatcttctc gaactcagga tcaaaacgca gcgtcaggtc ggtcaccagc attgtcggtt
    31141 tacgtttctt cgacggatcg aacggatccg ggataatttc cggtgcgtct accgcttcga
    31201 actggattgc tccagccgga ctgcgggtct gtacccactc atacttgaac agattctcga
    31261 agaaatagtt gctccactgg gtcggcgtct gggtccagac tacttccaga ccagaggtaa
    31321 tggcatctgc gccaacgccg ctgccgtaag tgctcgccca gcctaaacct tgttcttcaa
    31381 tcggtgcagc ttctggatca ggacctacgt ttgatgtcgg accggcaccg tgggttttac
    31441 ccagcgtatg accacccgca atcagcgcca cggtttcttc gtcgttcatg cccatgttgc
    31501 cgaaggtcgc gcggatagct gctgccgcag aaagcggttc gccgctgtga tccgggcctt
    31561 ccgggttaac gtaaatcagg cccatctcgg ttgcgcccag cggtgctttt gccagcgctt
    31621 ccggatgacg gtgagtcagc caggcttttt catcacccca gttaacgtcc agatccggtt
    31681 cccaaacgtc ttcacgaccg gcaccaaaac cgaaggtacg gaagccggag ttttccagcg
    31741 ccacgttccc cgcgaggata aacaggtctg cccaggagat tttctgacca tatttctgtt
    31801 tgattggcca caacaggcga cgcgctttat cgaggcttac gttatccggc caggagttca
    31861 gcggtgcaaa acgttgctga ccacgacccg cgccgccgcg tccgtcgatg gaacggtaag
    31921 tacccgcgcc gtgccaggcc atacgaataa ataagccagc gtagctgccc cagtcggcag
    31981 gccaccacgg ctgagattct gtcagcaggg ctttcagatc ttttttcagg ccgtagtaat
    32041 ctaatttgct gaattctttg cggtagtcaa agtcctcacc cagcgggtta gaacgattag
    32101 aatgttggtt taacaggtca acacggagtt gatttggcca ccagtcgcga gtggttgtgc
    32161 ccgcccccgc gctctggtcg tgaccgccct gatggaacgg gcatttgcca gtggctgtcg
    32221 tgttatggat atcgtctgac gtgctcatca atgtgctccc ctctacagtg ttaccgttac
    32281 gatacacagc gttagagaga agttataatt gaattaatcc acggatgcga tagttgagat
    32341 cttacatatt gttggttaaa gagatgtaga tcaaattgat cgtaattaag cttttttgat
    32401 tatgtccgaa ttcggacaaa tgctttataa aaaagggttt taggttcata tccttcaagt
    32461 aatgagggag aaaacaaggc tgataacctt attttcccgc cctcatttcg aggcagcatt
    32521 ttgtgctctg tttaaaattt gtgatcactg tgtgattttc acaaaagcca cactatttat
    32581 aaaccaggtc gaacacccag cgtatggcaa atcgcgtaac tcatttcagc gcggttaagc
    32641 gtatagaagt ggaaatcttt cactccttca cggcttaaaa tcttcaccat atccatggca
    32701 atattcgctc ccaccagttt gcgggtttcg gcatcgtcgt ccagcccgtc aaacatttgt
    32761 gccatccacg ccggaatacg cacgttggtc atatcggcaa atttcttcgc ttgtttaaag
    32821 ttcgagaccg gcaaaattcc cggaataatt tccacatcaa tacccgccga tacgcagcgg
    32881 tcacgaaaac gcaggtagct ttcgacatca aagaagaact gagtaatcgc gcggttggct
    32941 ccggcatcga ctttgcgttt caggttgagc aaatccgcct gagcgctttt tgcttccggg
    33001 tgaacctccg gatacgccgc cacggagata tcgaaatctg ccacttcttt taacagcgtc
    33061 accaggtcag aagcatacat ttccggctta ccacttcccg gcggcagatc gccacgcagc
    33121 gccacgatat gacgaatacc gttattccag tagtcgcgtg caatggtgcg cagctcgtcg
    33181 ggcgtcgcat caatgcaagt aagatgcggt gccgcttcca gaccagtgcg atctttaatg
    33241 cctttaataa tgctgtgcgt acggtcgcgc tcgccggagt tcgcgccata ggtcaccgat
    33301 acaaacttcg gtttcaggct gctaaggcga tcgatggagt tccacagggt ctgctccatt
    33361 tcactggtac gcggcgggaa aaactcgaac gaaacgttaa tctgcccctg gacttctgcc
    33421 aggctctgat tcagggcatc ccgctggctg gcgtgaaaaa agctcatacc ttacctcatc
    33481 aatcgcttgt agttatatgt tgtgttgtgc acatctatcc atttagacgt ccagatgtaa
    33541 agatgaagga aaagccgaag ggcgtcaacc gaaaaatgac caatgatggt gaggaaaaat
    33601 cagggaagat gaaaaaactt caggatggta agaaaaagaa aatccggtat gcaaagcaaa
    33661 ccggactgca aatataaact aaccatgtac ttattgcttc ttgccggatt cgtcgtaaac
    33721 gcttcatccg gcctaatcct atgataattc aatatatttc attagaaacc atagacccga
    33781 aaacatgagt accgggcatt attaaatttc tgaaattaca acaactgtgc cagccggttg
    33841 atatccgact gaatcgcccc ggcggtgacg tcgcgcccag cgccaggccc acggatcacc
    33901 agcggattat cgcggtacca gcggctttcg atggcaaaga cgttatcgca tggcagcagt
    33961 gatgccagcg gatggtcttc acgcaccgct tccacgccca cacgcgcttt gccattggca
    34021 tcgaaacgcg ccacgtagcg cagcaccagc cccatttcgc gggccgcttc cagccgttgc
    34081 accatctgct cgttaagttc atcgccattt tcaaagaaat ggtcgatgct gccgccttcg
    34141 caatgggtag gcaccagcga ctccacgcgt acctgatccg gttcgatgtt gtaacctgct
    34201 tcacgcgcca gaatcaccag cttgcgcatc acgtctttgc cagagagatc gtcacgcggg
    34261 tcaggttcgg ttaagccctg ctgccacgcc tgatccacca gctcggtaaa cggcacgcta
    34321 ccatcgaatt gcaggaacaa ccaggagagc gtgccggaga agatcccgct gatcgacaaa
    34381 atagtatcgc cgctgtcgat cagatcgcgc acggtgtggt tgatcggcaa gcccgcaccg
    34441 acggtggcat tgtacagcca gtgacgcccg gttttttcga aggcgtcgtg gatctggcga
    34501 tatttattgc tgtcgctggc tcccgccagt ttgttggcgc tgataacgtg gaaaccgtgg
    34561 ctggcgaaat caagatactg atcagcaagc tgctggctgg cggtaacgtc cagcaccact
    34621 aaatcatcat acggatgggc gcgcatccac aggaacaacg actcttcatc ctgctcaacc
    34681 gcttcatcgt tgaagaaggc taacgcgcgg ctggcgtcca gcccgtcata gctcaacagg
    34741 ctgcggcggc tgtccaccac acctgccagc acaaactcaa agccggtacg tgccgaaagc
    34801 gtgctctgct cacgggcgaa cagttccagc caacgggaac cgatattgcc cttaccgaac
    34861 aataccaggc cgatgcgttt ttctgcgcgg aagacggact gatgcagccc ctgaatcagg
    34921 ctttcggtcg ggccggtgcg cagtactgcc accaggctga tgccgtcatc ggactgccag
    34981 gtaaattcga ccggctggcc tttcagttgc tgccagaagc ggtggcaatg cagcgggtta
    35041 cgggtgacgc ctgcaccgac catcgccacc agcgccagcc cctgacgcag gcgcagttcg
    35101 ccaggtaatc ccgcttcgtc gaggattttc agcgcactgt cggccacttc tgaggtgtag
    35161 caaaattgca gcaactggcg atcgttatgt acgccaaccg ccagcgggcg tacctgcgcg
    35221 cgtttcagga tttggtcgat ctctttatgc gccagtttga aatcctgact ggcgggcacc
    35281 tgaaactcaa tcaaacagac atcatcgtgg ctggtgacaa tacgcgcacc agtaccggag
    35341 gccagcacgc gttcaatgcg cgtggaacct tgatccggcg tgtagctaca gcgcagttgc
    35401 aggtcgattt cgctgccaga aaccggctgt aaagtacggg cgtgaagaac gggagccgcc
    35461 aggcgcgcca gttcgctggc ctcatccaga cgcagcaacg gcagcaggca ggcatctttc
    35521 actttacgcg ggtcggcact gtataccccg gcgacgtcgc tccagatggt tacgcgagaa
    35581 acacccgcca gcgcaccgat ttgtgtcgcg gaatagtcgg aaccgttacg ccccagcagc
    35641 accgtttcac cggcgttgtt gcggctgata aatccggtca ccaccagacg tttgcccgga
    35701 tgttgcacca gcagctgttg cagcaacggg taagaaagcc cttcatcaac ctgcggttgt
    35761 gcggcgcgtt cagcgcgtaa aaactcgcgg gcatcaagcc aggccgctgg cagcccttgt
    35821 tgattaagta ccgcagacat caggcgtgcc gaccatactt ccccgtggcc caccacttcc
    35881 gcatacactg cgtcgttaat accgctgtcg agcagcgccg ccaggcgctc aaggtcgctg
    35941 acaaaagcgc taatgaggct atcggcttct tcagcgggta gcagaccgct aatcagatcg
    36001 cactgatagc gacgcagcgt ttgttgaacc tgatgcgcag agagacgatc ggtctggctt
    36061 agtttcaacc agttaatcaa ctggttagtg gtgctaccgg cggcggaaac caccatcata
    36121 tcgtcaggct gagagtactc cgccataatg cccgcgacac gcaaataaca cttcacatca
    36181 gccagactac tgccaccaaa tttatgcagc tgacgacctt tcgcccctgc ctgcgcaatc
    36241 acactcattt ttaccccttg tttgcagccc ggaagccatt ttccaagtcg gcaattaaat
    36301 cttcgccatc ttcaataccg gtggagatac gcagcagcgt ctcggaaatc ccggcggcag
    36361 cacgcgcttc tggtgccatg cctgcatgtg tcatggttgc ggcgtgagag attaaacttt
    36421 ccactccccc taatgattcc gccagcgtaa acaacgacag cccgcccagg aaacgacgca
    36481 gcgtctgctc atcgccatcc agttcaaaac tcaacattgc gccaaagcct ttttgctgac
    36541 gcgcggcaat ttcatgcccc tgattttccg gcagcgacgg gtgatacagt tttttcacca
    36601 acggctgggt ttgcagatat ttcacaatcg cctgcgcgtt gcgctgcgcc agctccatac
    36661 gcggcaccag cgttcgcaac ccgcgtagca gcagatagct gtcaaacgcg ccgcccgtca
    36721 cgccaatatt gtttgcccac caggcaagtt cagtgacaac gtccggttct ttagcaatca
    36781 ccacgccagc caccacgtca gagtgaccgt tcagatattt cgtgcatgaa tgcaacacca
    36841 gatcggcacc taatgccagc ggattttgta atgccgggct taagaaggtg ttatccacca
    36901 cgctcaccgc cccgacttcc cttgccagat ggcagatttt cgcaatatcc acgacgcgta
    36961 acaatggatt acttgggctt tctaccagta ccagtttggg tttttctgcc agcgctgccc
    37021 gtaatgcctg ttcatcgcct tgatcaacaa acaacacgcg atagcaaccg cgtttcgcca
    37081 gactgtcgaa caggcgatag ctaccgccgt agcagtcgtg cggcgcaacc agcagatcgc
    37141 caggtttcaa aaagacggtc gttaccaggt gaatcgcgga catgccggta ttagtaagta
    37201 ctgcaccagc accgccttcc agttctgcca gcgcacgctg aaccacatcg cgcgttgggt
    37261 tgccgcgacg cgagtaatca tgcgcgcgcg gttcattaaa tccggtaaag ttataggtgc
    37321 tggaaagatg gatcggtggg acaacgcaac catactgttc gtcgtcattt aacccgctac
    37381 gcactgcgat ggtggcctgt ttacgcgtca tgtgatgaag ttccctgggc tttgtcggtg
    37441 aaatgtcagg caccagagta aacattgtgt taatggacgt caatacatct ggacatctaa
    37501 acttctttgc gtatagattg agcaaatccc aaatagccgt taaaattata tgcattatca
    37561 cgccgacagg tgcattacac gatgtcacgg taacgcctgt acggtaaact atgcgggttt
    37621 acggtcagta cctacattaa ttgtgtggtc tgatctcaat ttattgacga agaggattaa
    37681 gtatctcatg gctgaatgga gcggcgaata tatcagccca tacgctgagc acggcaagaa
    37741 gagtgaacaa gtcaaaaaga ttacggtttc cattcctctt aaggtgttaa aaatcctcac
    37801 cgatgaacgc acgcgtcgtc aggtgaacaa cctgcgtcac gctaccaaca gcgagctgct
    37861 gtgcgaagcg tttctgcatg cctttaccgg gcaacctttg ccggatgatg ccgatctgcg
    37921 taaagagcgc agcgacgaaa tcccggaagc ggcaaaagag atcatgcgtg agatggggat
    37981 taacccggag acgtgggaat actaaagcgg aaaagagcag cgcggagtta acctgccgct
    38041 ttttttatac agggacgttt aacgatgaaa tacaggctac tgcccctcag cctgctggtt
    38101 tctgtacctg cctttgcctg gcagccgcaa accggcgaca tcatctttca aatctctcgc
    38161 tcatcgcaaa gtaaagcgat ccaactggcg acccattccg attacagcca caccggtatg
    38221 ctggtgatac gcaacaaaaa gccctacgtt tttgaagcgg tcggcccggt gaaatacacc
    38281 ccgctcaagc agtggatcgc ccatggtgaa aagggcaaat acgtcgttcg ccgcgttgaa
    38341 ggcggactga gtgttgaaca acagcaaaaa ctggcgcaaa cggcaaaacg ttatctcggt
    38401 aaaccgtatg atttcagctt ctcatggagc gacgatcgcc agtactgttc ggaagtggtg
    38461 tggaaggttt accagaacgc gctgggaatg cgcgtgggcg agcagcagaa gttgaaagag
    38521 ttcgatctca gcaacccgct ggttcaggca aagctcaaag aacgctacgg caaaaatatc
    38581 ccgcttgagg aaacggtggt ctcaccacaa gccgttttcg atgcgccaca actcaccact
    38641 gtcgccaaag agtggccgct gttttcgtgg taatggatga tgaaaaaaat aattctcccc
    38701 gtagtctgtt tgttgctttg tagttgcacg acctttccgc aagctgtcgc tccggtaaac
    38761 ggtgatgcct ccaattagtt gaattaagga tataatactc gcttttaagg ttgtttcatg
    38821 gtcacagtta accttcattg cccccgttgc cagtccgttc aggtttaccg tcacgggcag
    38881 aacctaaaaa gacatgagcg attccgcggc cgtgactgtc accgggtatt tcagctcagt
    38941 tactgttacg agccccgtaa accgggagtt aaagaccaaa tcgctgaaat gactttcaac
    39001 ggtgccgggg ttcgcgatac tgcccgtacg ctgaaaatcg gcattaatac agtcatccga
    39061 actttaaaaa actctcgcca cgaagaataa cgtcatcccc ggtcgctcat gctgatgtgg
    39121 cactcatctg caaacttagt cagcaatgga gctttgtcgg cagtaaagcg cgacagcact
    39181 ggctctggta cgtgtacaac acgaaaactg gtggtgttct ggcctacact ttcggccccc
    39241 ggactgacga aacctgtcgt gaattgcggg cgctgcttac ccttttacca tcggcatgtt
    39301 gaccactgac gaatggggta gttacgctag agagttaccg aaggagaagc atctgactgg
    39361 taagattttt actcagcaca ttgagcgtaa caatctgacc ctgagaaccc gaatcaaacg
    39421 tctggcccgc agaaccatct gtttctcgcg ctctgtagaa cttcatgaaa aagtcatcgg
    39481 tgcttttatc gaaaatatat gttctactaa ttggagtcat cacccttctg atgagctttc
    39541 cttggatgat taccagactg aatacacagc caaagatttt tacctcattc tgctcttttt
    39601 tctcaaatga c
//
LOCUS       DAZKHC020000037        39300 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZKHC020000037 DAZKHC020000000
VERSION     DAZKHC020000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 39300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.037"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            135..797
                     /gene="acrS"
                     /locus_tag="ACHOSM_RS11020"
                     /old_locus_tag="pgaptmp_002204"
     CDS             135..797
                     /gene="acrS"
                     /locus_tag="ACHOSM_RS11020"
                     /old_locus_tag="pgaptmp_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HGU4558719.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(800..979)
                     /locus_tag="ACHOSM_RS11025"
                     /old_locus_tag="pgaptmp_002205"
     CDS             complement(800..979)
                     /locus_tag="ACHOSM_RS11025"
                     /old_locus_tag="pgaptmp_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HGU4558720.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(1063..1947)
                     /gene="yhdJ"
                     /locus_tag="ACHOSM_RS11030"
                     /old_locus_tag="pgaptmp_002206"
     CDS             complement(1063..1947)
                     /gene="yhdJ"
                     /locus_tag="ACHOSM_RS11030"
                     /old_locus_tag="pgaptmp_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU4558721.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(2033..2329)
                     /gene="fis"
                     /locus_tag="ACHOSM_RS11035"
                     /old_locus_tag="pgaptmp_002207"
     CDS             complement(2033..2329)
                     /gene="fis"
                     /locus_tag="ACHOSM_RS11035"
                     /old_locus_tag="pgaptmp_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HGU4558722.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2355..3320)
                     /gene="dusB"
                     /locus_tag="ACHOSM_RS11040"
                     /old_locus_tag="pgaptmp_002208"
     CDS             complement(2355..3320)
                     /gene="dusB"
                     /locus_tag="ACHOSM_RS11040"
                     /old_locus_tag="pgaptmp_002208"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGU4558723.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3649..4530)
                     /gene="prmA"
                     /locus_tag="ACHOSM_RS11045"
                     /old_locus_tag="pgaptmp_002209"
     CDS             complement(3649..4530)
                     /gene="prmA"
                     /locus_tag="ACHOSM_RS11045"
                     /old_locus_tag="pgaptmp_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGU4558724.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4542..5993)
                     /gene="panF"
                     /locus_tag="ACHOSM_RS11050"
                     /old_locus_tag="pgaptmp_002210"
     CDS             complement(4542..5993)
                     /gene="panF"
                     /locus_tag="ACHOSM_RS11050"
                     /old_locus_tag="pgaptmp_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HGU4558725.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5983..6225)
                     /locus_tag="ACHOSM_RS11055"
                     /old_locus_tag="pgaptmp_002211"
     CDS             complement(5983..6225)
                     /locus_tag="ACHOSM_RS11055"
                     /old_locus_tag="pgaptmp_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HGU4558726.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6334..7683)
                     /gene="accC"
                     /locus_tag="ACHOSM_RS11060"
                     /old_locus_tag="pgaptmp_002212"
     CDS             complement(6334..7683)
                     /gene="accC"
                     /locus_tag="ACHOSM_RS11060"
                     /old_locus_tag="pgaptmp_002212"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGU4558727.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7694..8164)
                     /gene="accB"
                     /locus_tag="ACHOSM_RS11065"
                     /old_locus_tag="pgaptmp_002213"
     CDS             complement(7694..8164)
                     /gene="accB"
                     /locus_tag="ACHOSM_RS11065"
                     /old_locus_tag="pgaptmp_002213"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGU4558728.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9030..9860)
                     /locus_tag="ACHOSM_RS11070"
                     /old_locus_tag="pgaptmp_002214"
     CDS             complement(9030..9860)
                     /locus_tag="ACHOSM_RS11070"
                     /old_locus_tag="pgaptmp_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4558729.1"
                     /translation="MTSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLSEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTKQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(9897..10871)
                     /locus_tag="ACHOSM_RS11075"
                     /old_locus_tag="pgaptmp_002215"
     CDS             complement(9897..10871)
                     /locus_tag="ACHOSM_RS11075"
                     /old_locus_tag="pgaptmp_002215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HGU4558730.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            11023..12963
                     /gene="csrD"
                     /locus_tag="ACHOSM_RS11080"
                     /old_locus_tag="pgaptmp_002216"
     CDS             11023..12963
                     /gene="csrD"
                     /locus_tag="ACHOSM_RS11080"
                     /old_locus_tag="pgaptmp_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HGU4558731.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13268..14311
                     /gene="mreB"
                     /locus_tag="ACHOSM_RS11085"
                     /old_locus_tag="pgaptmp_002217"
     CDS             13268..14311
                     /gene="mreB"
                     /locus_tag="ACHOSM_RS11085"
                     /old_locus_tag="pgaptmp_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HGU4558732.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            14377..15480
                     /gene="mreC"
                     /locus_tag="ACHOSM_RS11090"
                     /old_locus_tag="pgaptmp_002218"
     CDS             14377..15480
                     /gene="mreC"
                     /locus_tag="ACHOSM_RS11090"
                     /old_locus_tag="pgaptmp_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGU4558733.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            15480..15968
                     /gene="mreD"
                     /locus_tag="ACHOSM_RS11095"
                     /old_locus_tag="pgaptmp_002219"
     CDS             15480..15968
                     /gene="mreD"
                     /locus_tag="ACHOSM_RS11095"
                     /old_locus_tag="pgaptmp_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGU4558734.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15977..16570
                     /gene="yhdE"
                     /locus_tag="ACHOSM_RS11100"
                     /old_locus_tag="pgaptmp_002220"
     CDS             15977..16570
                     /gene="yhdE"
                     /locus_tag="ACHOSM_RS11100"
                     /old_locus_tag="pgaptmp_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HGU4558735.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            16560..18029
                     /gene="rng"
                     /locus_tag="ACHOSM_RS11105"
                     /old_locus_tag="pgaptmp_002221"
     CDS             16560..18029
                     /gene="rng"
                     /locus_tag="ACHOSM_RS11105"
                     /old_locus_tag="pgaptmp_002221"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HGU4558736.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            18097..21897
                     /gene="yhdP"
                     /locus_tag="ACHOSM_RS11110"
                     /old_locus_tag="pgaptmp_002222"
     CDS             18097..21897
                     /gene="yhdP"
                     /locus_tag="ACHOSM_RS11110"
                     /old_locus_tag="pgaptmp_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HGU4558737.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNLGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            22052..23497
                     /gene="tldD"
                     /locus_tag="ACHOSM_RS11115"
                     /old_locus_tag="pgaptmp_002223"
     CDS             22052..23497
                     /gene="tldD"
                     /locus_tag="ACHOSM_RS11115"
                     /old_locus_tag="pgaptmp_002223"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HGU4558738.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(23625..24554)
                     /gene="aaeR"
                     /locus_tag="ACHOSM_RS11120"
                     /old_locus_tag="pgaptmp_002224"
     CDS             complement(23625..24554)
                     /gene="aaeR"
                     /locus_tag="ACHOSM_RS11120"
                     /old_locus_tag="pgaptmp_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HGU4558739.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            24737..24940
                     /gene="aaeX"
                     /locus_tag="ACHOSM_RS11125"
                     /old_locus_tag="pgaptmp_002225"
     CDS             24737..24940
                     /gene="aaeX"
                     /locus_tag="ACHOSM_RS11125"
                     /old_locus_tag="pgaptmp_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HGU4558740.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24948..25880
                     /gene="aaeA"
                     /locus_tag="ACHOSM_RS11130"
                     /old_locus_tag="pgaptmp_002226"
     CDS             24948..25880
                     /gene="aaeA"
                     /locus_tag="ACHOSM_RS11130"
                     /old_locus_tag="pgaptmp_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HGU4558741.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25886..27853
                     /gene="aaeB"
                     /locus_tag="ACHOSM_RS11135"
                     /old_locus_tag="pgaptmp_002227"
     CDS             25886..27853
                     /gene="aaeB"
                     /locus_tag="ACHOSM_RS11135"
                     /old_locus_tag="pgaptmp_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HGU4558742.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGALFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27945..28217
                     /locus_tag="ACHOSM_RS11140"
                     /old_locus_tag="pgaptmp_002228"
     CDS             27945..28217
                     /locus_tag="ACHOSM_RS11140"
                     /old_locus_tag="pgaptmp_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HGU4558743.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(28273..28536)
                     /gene="yhcN"
                     /locus_tag="ACHOSM_RS11145"
                     /old_locus_tag="pgaptmp_002229"
     CDS             complement(28273..28536)
                     /gene="yhcN"
                     /locus_tag="ACHOSM_RS11145"
                     /old_locus_tag="pgaptmp_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HGU4558744.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(28900..29370)
                     /gene="argR"
                     /locus_tag="ACHOSM_RS11150"
                     /old_locus_tag="pgaptmp_002230"
     CDS             complement(28900..29370)
                     /gene="argR"
                     /locus_tag="ACHOSM_RS11150"
                     /old_locus_tag="pgaptmp_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HGU4558745.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            29805..30743
                     /gene="mdh"
                     /locus_tag="ACHOSM_RS11155"
                     /old_locus_tag="pgaptmp_002231"
     CDS             29805..30743
                     /gene="mdh"
                     /locus_tag="ACHOSM_RS11155"
                     /old_locus_tag="pgaptmp_002231"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU4558746.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(30805..31872)
                     /gene="degS"
                     /locus_tag="ACHOSM_RS11160"
                     /old_locus_tag="pgaptmp_002232"
     CDS             complement(30805..31872)
                     /gene="degS"
                     /locus_tag="ACHOSM_RS11160"
                     /old_locus_tag="pgaptmp_002232"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HGU4558747.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(31962..33329)
                     /gene="degQ"
                     /locus_tag="ACHOSM_RS11165"
                     /old_locus_tag="pgaptmp_002233"
     CDS             complement(31962..33329)
                     /gene="degQ"
                     /locus_tag="ACHOSM_RS11165"
                     /old_locus_tag="pgaptmp_002233"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HGU4558748.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVSSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(33483..33881)
                     /gene="zapG"
                     /locus_tag="ACHOSM_RS11170"
                     /old_locus_tag="pgaptmp_002234"
     CDS             complement(33483..33881)
                     /gene="zapG"
                     /locus_tag="ACHOSM_RS11170"
                     /old_locus_tag="pgaptmp_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HGU4558749.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            34075..35202
                     /gene="zapE"
                     /locus_tag="ACHOSM_RS11175"
                     /old_locus_tag="pgaptmp_002235"
     CDS             34075..35202
                     /gene="zapE"
                     /locus_tag="ACHOSM_RS11175"
                     /old_locus_tag="pgaptmp_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HGU4558750.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRRRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGAENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            35421..35849
                     /gene="rplM"
                     /locus_tag="ACHOSM_RS11180"
                     /old_locus_tag="pgaptmp_002236"
     CDS             35421..35849
                     /gene="rplM"
                     /locus_tag="ACHOSM_RS11180"
                     /old_locus_tag="pgaptmp_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGU4558751.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            35865..36257
                     /gene="rpsI"
                     /locus_tag="ACHOSM_RS11185"
                     /old_locus_tag="pgaptmp_002237"
     CDS             35865..36257
                     /gene="rpsI"
                     /locus_tag="ACHOSM_RS11185"
                     /old_locus_tag="pgaptmp_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGU4558752.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            36651..37289
                     /gene="sspA"
                     /locus_tag="ACHOSM_RS11190"
                     /old_locus_tag="pgaptmp_002238"
     CDS             36651..37289
                     /gene="sspA"
                     /locus_tag="ACHOSM_RS11190"
                     /old_locus_tag="pgaptmp_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HGU4558753.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            37295..37792
                     /gene="sspB"
                     /locus_tag="ACHOSM_RS11195"
                     /old_locus_tag="pgaptmp_002239"
     CDS             37295..37792
                     /gene="sspB"
                     /locus_tag="ACHOSM_RS11195"
                     /old_locus_tag="pgaptmp_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HGU4558754.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(37836..39203)
                     /gene="dcuC"
                     /locus_tag="ACHOSM_RS11200"
                     /old_locus_tag="pgaptmp_002240"
     CDS             complement(37836..39203)
                     /gene="dcuC"
                     /locus_tag="ACHOSM_RS11200"
                     /old_locus_tag="pgaptmp_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU4558755.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMYVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9566 a         9796 c        10273 g         9665 t
ORIGIN      
        1 aatatatatt atttatttga cgatttatag caactcaata ttagcctctc gtataaatac
       61 acattaggtg atagattaac cttcgctatt ttctcactct gtgtcgaata tatttatttc
      121 ctgaataatt aatcatggca aaaagaacca aagccgaagc tctgaagacc cggcaagaac
      181 tgattgaaac tgccatcgcc cagtttgcgc agcatggcgt aagcaagacg acactcaacg
      241 acattgccga cgctgctaac gttacgcgtg gcgctatcta ctggcacttc gaaaacaaga
      301 ctcaactgtt taatgagatg tggttgcaac agccttcatt gcgagagtta atccaggatc
      361 acttgacggc tggattagag catgacccgt ttcaacaatt gcgtgaaaaa ttgattgtcg
      421 gcttgcaata tattgccaaa attccccgcc agcaggcgtt gctgaaaatc ttatatcaca
      481 aatgtgaatt taatgatgag atgctggccg agggagtgat acgcgaaaag atgggcttta
      541 atccgcagac tctccgcgaa gtattgcagg cgtgtcagca acaaggttgt gtagcaaata
      601 acctcgattt agatgttgtg atgattatta ttgatggtgc cttcagcgga attgttcaaa
      661 actggttaat gaatatggcg ggttatgatc tttataaaca agcacccgcg ctggtcgata
      721 acgtattaag aatgttcatg ccagatgaaa acataacgaa attaattcat caaacgaatg
      781 aattaagtgt catgtaaatt cacattctgt cctgaaaatt cagttcgtca cagttaacca
      841 gttttagtgg attaactgag ttcatattac ggcatttgtc tgtttctgtt gtcagcagtt
      901 caatccactg catcagtaaa gtatcaaaca gaaaaacgaa gacagcgaaa acgacgagcc
      961 accaatactt gcgaatcatt acctgagcct gtgcatgttt aaccaagtct ggcaggaaat
     1021 atacacgaaa tgaataatct gaaaaccagg cttacacttc ctttactttg caatgagatc
     1081 ggggtcaact tcgcttaacc ggcttcgttt tgacaggttg ccggtctttc tttttttcac
     1141 tttcgccagt tcttccgcag agtaatgcga cgcgacatcc agccgtcgaa gccccatttt
     1201 gatgtactcg ctgttgatct caataccaat gaattttcgc ccgctggaga cggctacggc
     1261 accggtagta aagctaccgg caaacgggtc gagaacgata tcgcctgggt tggaagaggc
     1321 gagaataatg cgtttcagta aggcttccgg tttttgcgtc gggtggttct catattcatc
     1381 catcaaataa cgcacgcgcg gaaaatccca gacgttaccc ggtacttttt gatgattgta
     1441 tggctgtgga ggatttttgc gataatcgat caacgcgcgc tgcgatccgg ttttggcttc
     1501 taccagaata gcatcaccgt tgaatgtgta gttctttgcg tctttcacca tcatcaggat
     1561 gggttcgtac atggagccgt agtgtttttt cgcctgtact ccagaactgt catatgacca
     1621 gacgatgcga cttttgatgg taaaaagctt gcggcactgg agatcgataa agggcatgtt
     1681 ttccgtgctg ttcatgatgt acatgctgcc ctgctttttc agaacgcggt ggcactctgc
     1741 aatcacttca aacagccagt cgataaacag atcttctttc caggcttcga tcagaccatc
     1801 aaaattttta ccgatgttat atggtgggtc ggcaaagatc agatcgacac tttcggcggg
     1861 gatcttttta agttcggcaa gcgcatcacc gtgaataatg gtcttagctt cattaccaaa
     1921 ccgggtcggt tcacatcctg ttctcatggt cactcccttt gtgacaccta taaaaaaggc
     1981 gcttccccat gccgagtagc gcctttttaa tcaagcattt agctaacctg aattagttca
     2041 tgccgtattt tttcaatttt ttacgcagcg taccacggtt gatgcccatc atcagcgcag
     2101 cacgggtctg gttaccacgg gtgtattgca tcaccatgtc caacaggggc tgttctactt
     2161 cagccagtac cagctcatag aggtcattca catcctgacc attcagttga gcaaaatagt
     2221 tcttcagtgc ctgtttaacc gagtcacgca ggggtttttg ggttacctga tcctgagagt
     2281 taacggtaga aacggtcagt acgtcagaat ttacgcgttg ttcgaacata gttctgtcag
     2341 ctctttattt ctgtttacgc aaaattttcg aagtatgcct ccaacgcctc cagctgttcg
     2401 ctggcatcct caatggcgtt gaatgtgcgc cgaaactggt catttggagc gtgttcctgg
     2461 agataccagg aaacgtgttt acgtgcaatt cggtaccctt ttgccggacc ataaaagtca
     2521 tgcagttccc gaacgtgcgc gcaaagcaag cgcttaacct ctgccaaagg cagcgggggc
     2581 agcaactccc cagtgtccag ataatgctgg atttcccgaa agatccaggg tcttccctga
     2641 gctgcgcggc ctatcatcag ggcatccgcc cctgtatagt cgagcacagc tctggcttta
     2701 agcgggtcag taatgtcgcc attcgcgata accggaatgg aaactttctg cttaactgcc
     2761 cgaatactgt cgtactcagc ttctccattg aacaaacagg cgcgtgtacg gccatgaatg
     2821 gtcagagcct gaatgccaca gtcttcagcc agttgggcaa tctcttcgca gttacggtgt
     2881 tccggtgccc agccggtgcg aatcttcagg gtaacaggaa cgtccactgc attgacgacc
     2941 tcggtaagga tcgatttaac gacatccggg tactgcaaga gggctgaacc tgcgagcttg
     3001 cgattcactt ttttagccgg gcaacccata ttgatatcaa taatctgggc accgctttcc
     3061 acgttaatac gtgctgcatc tgccatttct ttcggatcgc taccagcaat ttgcacggtg
     3121 cgaataccgg gttcatcaat gtgcaccatc cgtaaacgag atttgtcgct ttcccaaacc
     3181 tgtgggttag aagacatcat ctcggatact gtcaatccgg ctcccatctc gtagcacaac
     3241 gtccgaaaag gtctgtctgt aatgccagcc atgggcgctg cgatcaggcg atttctgagc
     3301 tgatattgtc cgatgcgcat gagttaagaa atgaccatac tgtgactgca aggcggcgta
     3361 tattacgcat tttttgcacg agatgaaagg ccaaactttg accaatcctc tgagatggat
     3421 caaagaattg catttaaaat gagcgtggtg cgataattac tcataaaaat catcatatta
     3481 gaaaatagtg actaaaattt acactcaaag aaatttgagt aagttctcaa tttttcttta
     3541 tgaatgaaaa ttttggcacg caaatttgcg taaataatcg gcaaatttac gtgccttttg
     3601 tgagcttgct cgcacttcgc cccgcgtcac cctacggcga tgcgaaggtt aattcttacg
     3661 accggtaata cggcaccact cttctttttc cacgaccggg tccagtgcga agctatcggc
     3721 ataagcttca caaacgctct ctgcctggct tgccagaata ccggaaaggc ccagcaaacc
     3781 gcctgaaacc ggcaggacgc tgattaacgg tgccagttca cgtaatgggc ctgcaaggat
     3841 gttcgcgacc accacgtcgg ctttcatttc ttctggctga tctttcggta agtagagttc
     3901 cagacggtca gaaacgccat tacgttcggc gttgtcgcgg ctagcctgaa tcgcctgcgg
     3961 atcgatatca ataccaatgg cttttgctgc acccagtttc agcgccgcga tagccagaat
     4021 gccggaaccg cagccaaagt cgatgactgt tttaccagtt aaatcgaggc tgtcgagcca
     4081 ttgcaggcac agagaggtag ttggatgggt acccgtacca aacgccagcc ctgggtctaa
     4141 catcacgttg acggcgtttt cgtccggcac atcacgccag cttgggcaga tccacagtcg
     4201 ttcaccaaag cgcatcgggt ggaaattatc catccattcg cgctcccagt ctttatcttc
     4261 cagttgttcg attttatgcg cgaagcctgc gccgagcagc ggatggtttt ccagaatcgc
     4321 caccacgtcg ttcatatcgg tttcagcgtc gaacagacca atcacatcgg tgtcgcccca
     4381 caggcgcgtt tcgcccggca gcggctcaaa tactggcgta tcgtgggtat cctgaaaagt
     4441 gatagaaacg gcacccgctt ccatcagcgc atcgctaaga tcttcagcgt tcgcgccggt
     4501 ggtgtttagt ttcagttgga tccaaggcat ggcaaaactc tttatttatc agtagtcaaa
     4561 acggtagctt gcgggacgga tgtaccgaaa cggtttccga ccaggaaagc cagcaaactt
     4621 agtagtaacg agggcacgat agggtggaag cccaggtact gaatattcag cgtcgcgagt
     4681 acggcataca gcacgccgcc aacgatcatc gcacttagcg cgcctttggc gttggcgcgt
     4741 tcccagtaaa gacccagcac cagcggccac aggaaaacgg cttccagccc accgaaggcc
     4801 agcaaattca gccagatgat catttctggc ggcttccagg cggcaagcag cagcaacgcg
     4861 ccgagaacta acgtaattac cgccgacatc cgcttcagac gcgtctcgtt ttgcatttga
     4921 tccggacgga tattcagata gagatcttta atgatcgtag cggaactttg cagcaactgg
     4981 gcattgatcg tcgacatgat cgcagccata ggtgcagcca ggaagattcc ggcagcaaac
     5041 ggtggcagca cttttaccat taacgtcggg atcaccagat ccggtacggt gagatcgggg
     5101 atcactgcac gacctaacgc tccggccagg tgcataccga acatcaggat tgcgactaca
     5161 atcgtaccga tgatgatccc ccggtgtact gctttgctgt ctttataaga gatacagcgc
     5221 accgcagtat gcggcaggcc aatcacgcca aagcacacca gtacccagaa cgacgtcata
     5281 aaggcaggcg acagaatatc gtcagcgcct tgtggcgtaa ccagttgcgg atcgatggtt
     5341 tgcaaggtct gtactgcgtt gcttaagccg ccagcggcat gtaccacgcc aataagcagc
     5401 acaacggtgc caatcagcat cacaagccct tgcatggtgt cgttcagcac gctggcgcga
     5461 aagccaccaa aggcggtata taacgcaatg ctgataccaa aaatcagcag cccggtttca
     5521 taaggaatac ccgccgcggt ttccagcagg cgcgcaccgc cgataaactg cacggtcatc
     5581 gcaccaacga acgcaaccag caaactcaaa ctcgccagcc acaccagaag acgactctgg
     5641 tagcgggcaa acagcatatc gttcagcgtc actgcattgt agcggcgcgc aagaatcgca
     5701 aacttcttgc cgagaatacc gagtgaaagc cagactgcag gaagctgaat catcgccagc
     5761 aatacccagc ccaggccgta tttataagca gctcctggcc cgccgataaa cgaactggca
     5821 ctgatatagg tcgcggtgag cgtcatcgcc agcacaatac cgcccataga gcggctgccg
     5881 aggaaatact cattaaggaa ggtgccggtg ctccgtttac gcatcgcata aacagagata
     5941 ccgaacacca ccaccagata ggcgaccagc ggtagaatta cttcaagctg catcgtcatc
     6001 ctccagtggg atatcgcgat agataaattt caccatcgcc cagcacagtc caataaacag
     6061 cagcggcgtc aggatgcagg ccatctcaaa ccagcgcgga aagccggtaa aaccgggggc
     6121 aacgccaggt aagtaagcgg ctactaacca taccgccaga tacaaaaggg tcagccccag
     6181 cgcccagcgc gcctctttat gggcctgaac aaaacgagtg tccatttttt gtccctgatg
     6241 ggtgaagaaa gcgaggattg taccttatgg gggttgtcga tccccagtaa taaaaaaggc
     6301 cggaaaatcc ggccttttga cgcttttgca gtcttatttt tcctgaagac cgagtttttt
     6361 ctccagatag tggatgttag tgccaccatg ctggaagttc tcgtcattca tgatgcggat
     6421 ctgcagatca acgttggttt tgataccgtc gatgatcagc tcctgcagcg cattcttcat
     6481 gcgggcaatc gccacgtcac ggttttcacc gtagcaaatc agcttaccga tcattgagtc
     6541 atagtacggc ggtacggtgt agcccgcgta gatatgagac tcccaacgta cgccaaaacc
     6601 gccaggtgcg tggaaacggg tgattttgcc tgggcttggc aggaaggtgt tcggatcttc
     6661 ggcgttgata cgacattcca ccgcatggcc gcgaacgtga acttcttctt gcttgatcga
     6721 cagcggttga ccggcagcga tacgcagctg ttctttgatc aggtcaacgc cggtgatcat
     6781 ttctgtaacc gggtgttcta cctgaatacg ggtgttcatt tcgatgaaat agaactcgcc
     6841 gttttcgaac aggaactcga aagtacctgc accgcgatag ccgatatcaa cacacgcttt
     6901 agcgcaacgt tcgccgatgt agcgacgcag ttccggggta atgcccggtg ctggtgcttc
     6961 ttcgaccact ttctggtggc ggcgctgcat ggagcagtca cgttccgcca gatagatagc
     7021 gttgccctga ccgtcagcca gtacctgaat ctcgacgtgg cgaggatttt ccaggtattt
     7081 ctccatgtaa accatatcgt tgctgaaagc agctttcgct tccgcacggg tcatggagat
     7141 ggattgtgcc agttcagcgt cgccgcgcac tacgcgcata ccgcgaccgc cgccgccgcc
     7201 ggaggctttg ataatcaccg gataaccaat gcgtttagca atggcgcggt ttttatccat
     7261 atcgtcgccc agcgggccgt cagaacccgg tacgcaagga acgcccgctt ttttcatcgc
     7321 cgcgattgcg gatactttgt cgcccatcag gcgaatggtt tctgctttcg ggccgatgaa
     7381 gataaagccg gagcgttcaa cctgctcggc aaagttggcg ttctcggaga ggaagccgta
     7441 acccggatgg attgctactg cgccggtgat ttcagcggcg ctgatgattg ccgggatgtt
     7501 cagataactt tttactgacg gagcagggcc aatacagacc gtttcatctg ccagtaatac
     7561 gtgttttaga tcgcgatccg cgctggagtg cacagcgaca gtcttgatgc ccagttcttt
     7621 acaggcacga agaatacgca atgcaatctc gccgcggttg gcaataacaa ttttatccag
     7681 catgttcgcc tcgttactcg atgacgacca gcggctcgtc aaattctacc ggttgtccac
     7741 tttcgaccag aattgctttc acggtaccgg atttgtccgc ttcgatctgg ttcatcattt
     7801 tcatggcttc aacgatgcac agggtatcgc ccacgttgac tttctgaccc acttcgatga
     7861 acgcttttgc gtccgggctt ggggtgcggt agaaagtacc aaccataggg gagcgtacga
     7921 tgtgaccact gatttccgct gctgctggcg cttccatgga aggaacggtc gccggagcgg
     7981 ctgcgttaga ttgagctggc tgctgcatca ttggtgcagc gtaagcttgt tgcatcacag
     8041 ggaaacttgc ggcaggagct gcacggctaa tgcgtactga ctcttcgcct tcagaaattt
     8101 ccagttcgga gatgcctgat tcttcaacca gctcgatcag ttttttaatc ttacgaatat
     8161 ccatgagtgg gttccgtact ctttgtttag tgtgattgtg acaggcgttt caccgccgtc
     8221 tgtaaagcgt atgccccgac agctgtatgc atagcgataa attccagcag gccgggacgc
     8281 cggtctattt tgccccagag tgtcaatgtt agacttgacg gacattgtgc agcgatctgc
     8341 tgctatcctc cggcaaaaaa caaaatatac cttcgttgcg ccaatgtcac cttttccgca
     8401 gcgcaaaaac tgcgtcaggg aggacgaggt cgcacattat aacgatttcg tagcaattgg
     8461 cagctaaata ctggtcttat cagggaagat aatcaacagc taacatgtaa ataaccttca
     8521 acaccgtgta atttgcaaca agccgcacaa ttcacgaaat tagcgccatc agcgacggaa
     8581 cttcttataa cgtaaggtga aaagctgtaa agccagccct gcgaagatga ggggctgcaa
     8641 tgagataatc ttcacagacc acaaataatg tagggatgcc aggatcgcga caagatagac
     8701 gacattacgc agcggttgct ggggattacc caattttcac tgcattgtct ggatagatgt
     8761 gaatgctaaa gcacgcaaaa ttaaccgatt gacaatgctc agcattaaat aggttcgagt
     8821 gattaatttg tagtcaaaca aaatcagatt attcccccca ttttgcagaa ggtaaactgt
     8881 tttgtcaggc ggcagtgcgg ctatctgatg ctttcagaat ttgccacctg acaataaaaa
     8941 agccgatttg gtagcaaaat cggcttcggt gagtttgcag ttttcatcac atactccgac
     9001 aagatttttc aagcatgacg gaggttgcgt taatgcaagg tttctaccca aggataaaag
     9061 tgaccgttac ggcggatgaa tatctgtgtt gtcagaggat ggtcatcctc acggaagcat
     9121 tgttttgtta gctgtcgtaa tgcactgaga tttgctggct gatcattgtg ggtgaaaaac
     9181 agttttcctc tggagggaac ggtcactaac aggtcgccgc attcaggatc gggtgattgc
     9241 acccaaagat cgtcaatgag catcagactg ggctcataag taccccctgc ggagataacg
     9301 gcaacttttc catcgtttgc gcctgtatca acgatttcag ccgtaacttc cggcaatatt
     9361 tgcttcaaat tactgatggc ttgttcgcgg tcaatggtca cttcgtagcg catcagatag
     9421 cgcatggttt cgctaccatc ttcagcaaac aagataatta actcatcgtt aaagtcttcc
     9481 caggcaaaat ccagcgaggc gttgaggacc tctggatcag actcatactg gctaagctgt
     9541 tggcgaacgg tatccagcca ggctttttct ttaatcaccg gaacaataca gcgcccggtc
     9601 ggcagcattt ctgaaagctg tttcagcata tctgcagcca cttcttgcag cgggtctggt
     9661 gcggcttgat aacgtttgta ggcatcatca agggtgattt ccaccgtttg gtgacccgta
     9721 tcaatgcgca tcaatttgtg gtcagtcgaa atcatgactt tccagtttgg atgatgttgt
     9781 acaacttcct gaccaaggtg ttcgacgaaa gctgtccggc taaccgcttt tcccgttaac
     9841 cgattgaata atgatgtcaa aatgagttcc ctctcttatt attccttgct aaatggttag
     9901 ttaaccttca ccagcgtgcg accctggatc tggttattaa tgatggcctc ggcaaagttc
     9961 ggtgcctctg acagagatat ctcttttgcc gcctgggtat agaatgattc cggtaaatcg
    10021 gcgaccagtc gctgccaggc ttgtgcgcgg cgttctggtg gcgtcattac tgaatccacc
    10081 ccttgcaaac ggacattacg cagaataaat ggcatgaccg tggttggcag agtaaaacca
    10141 cccgccagac cacaggccgc cacgcagccg ccgtaattca tttgcgccag cacttttgcc
    10201 agcactttgt cgccaacagt gtcaattgcc ccagcccaga cttgtttttc cagaggacgg
    10261 gattcggcaa actcatcacg agggagaata cgactggcac ctaaactttt cagatattca
    10321 tgggtacttt cgcgaccgga aacggcaacg acctgataac ccaacttatg cagcagcgcc
    10381 acggcggtac tgccgacgcc accactggca cccgtcacga caatctcccc gtcctgcggg
    10441 cgaacaccgg catcttccag cgccatcaca cacagcatgg cggtaaaacc ggcagtaccg
    10501 ataatcattg ctttacgcgc gtccagccct tgcggcatgg caaccagcca gtcacctttc
    10561 actcgcgcct gctccgccag cccaccccag tggttttcac caacgcccca gccagtgagt
    10621 aacacctcct gaccggcatg aaaacgcgga tcttcgctgg tgcgtacagt tccggcaaaa
    10681 tcgatcccag gaatcatcgg aaaattacgg atgatttttc ccttaccggt aatcgccagc
    10741 gcatctttat agttcaggct cgaccagtga acatcaaccg tgacatcgcc ctccggcagg
    10801 cgactttcgt caagtgtctg taccgatgcg agagttttgc cgtcctgctg ttctaaaagt
    10861 aacgcctgca taagtggtcc tcatgtgcat gatggattgg aaaataattt ctgaagacta
    10921 tactcgctaa tggaaaagca aagccgatga agcgcaataa attgcgagat aaatctgatt
    10981 tgctagtatg cccgcttcct cactatcgga gttaacacaa ggatgagatt aacgacgaaa
    11041 ttttcggcct ttgttacgct gctcaccggg ttaacaattt ttgtgacttt gctgggctgt
    11101 tcgctaagtt tctacaacgc aattcagtat aagtttagtc atcgcgttca ggcggtggcg
    11161 acggcgattg atacccacct tgtgtcgaat gacttcagca cattaaggcc acaaattacc
    11221 gaattaatga tgtcggcaga tatcgttcgt gtagacctac tccatggtga taaacaggtt
    11281 tataccctgg ccagaaatgg tagttatcgt ccggttggct ccagcgatct gtttcgcgaa
    11341 ctgagtgttc cgttgataaa gcatccgggg atgtcgctgc gtctggttta tcaggatccg
    11401 atgggcaact atttccattc tttaatgact accgcgccgc tcacgggggc gattggcttt
    11461 atcattgtta tgctcttcct ggcggtacgc tggttacaac ggcaacttgc cgggcaagaa
    11521 ttgctggaaa cccgggctac tcgtatctta aacggtgagc gtggctctaa tgtgttggga
    11581 accatctatg aatggccgcc cagaaccagc agtgcgctag atacgctgct tcgtgaaatt
    11641 cagaacgcac gcgaacaaca cagccgtctt gatacgctga tccgctctta tgccgcccag
    11701 gacgtgaaaa ccggcctcaa taaccgactc tttttcgata atcagttagc aacgttactg
    11761 gaagatcagg agaaagtagg tacccacggg atcgtgatga tgattcgtct gccggatttc
    11821 aatatgttga gcgatacttg ggggcacagc caggttgaag aacagttctt cactctgacg
    11881 aatctgctgt cgacatttat gatgcgctac cctggcgcac tgctggcgcg ttaccaccgc
    11941 agtgattttg ctgcgctgtt accgcaccgg acgttaaaag aggcagagag catcgccggt
    12001 cagttaatca aagccgtcga taccttgccg aacaataaaa tgctcgatcg cgacgatatg
    12061 atccacattg gtatctgcgc ctggcgtagt ggtcaggata ccgagcaggt aatggaacat
    12121 gcagagtctg ccacgcgtaa tgcgggattg cagggcggca atagctgggc tatttacgat
    12181 gactcgttgc ctgaaaaagg acgcggtaat gttcgctggc gtacgcttat cgagcaaatg
    12241 ctcagtcgcg gcggcccgcg cctttatcaa aaaccggcgg ttactcgcga aggtcaggtt
    12301 catcatcgcg aactcatgtg ccgcatcttc gatggtaatg aagaggttag ctcggcggag
    12361 tatatgccga tggtcttgca gtttggctta tcggaagagt atgaccgtct gcaaatcagc
    12421 cgtcttattc cactattgcg ttactggcca gaggaaaatc tggcgattca ggttaccgtt
    12481 gagtcgctga ttcgcccgcg ttttcagcgt tggctgcgcg atacgttaat gcaatgtgaa
    12541 aaatcacaac gaaaacgcat aattattgaa cttgcagagg ccgatgtagg tcaacatatc
    12601 agtcgtttac aacctgttat tcgtttagtg aatgctttag gggtacgggt agccgtcaac
    12661 caggctggtt tgacgctggt aagcaccagt tggatcaaag aacttaatgt tgagttactc
    12721 aagctccatc cggggctggt cagaaacatt gagaagcgaa cggagaacca gctgctggtt
    12781 caaagcctgg tggaagcctg ctccgggacc agcacccaag tttacgccac cggtgtgcgt
    12841 tcgcgaagcg agtggcagac cctgattcag cgcggtgtta caggcgggca aggggatttt
    12901 ttcgcgtcct cacagccact tgatactaac gtgaaaaaat attcacaaag atactcggtt
    12961 taacctgccg tttaatccgt tttcacgtag aataacgcgc gctgcgtctc atgggagtgt
    13021 gcttgtctgc tcgccagatt gttgcagcac atatgcagat gaatgacctt acgcggttgc
    13081 aaacaggcga ggaatgctgc tgatgcatta agcctttctg gactcaggca gagatttgta
    13141 acaaaggaaa cgaactgcac taattttcac cgtagcagat gatttttgcg ccttgtcgct
    13201 gctgcgtgtg gttggtaaag taagcggatt ttcttttccg ccccagcttt caggattatc
    13261 ccttagtatg ttgaaaaaat ttcgtggcat gttttccaat gacttgtcca ttgacctggg
    13321 tactgcgaat accctcattt atgtaaaagg acaaggcatc gtattgaatg agccttccgt
    13381 ggtggccatt cgtcaggatc gtgccggttc accgaaaagc gtagctgcag taggtcatga
    13441 cgcgaagcag atgctgggcc gtacgccggg caatattgct gccattcgcc caatgaaaga
    13501 cggcgttatc gccgacttct tcgtgactga aaaaatgctc cagcacttca tcaaacaagt
    13561 gcacagcaac agctttatgc gtccaagccc gcgcgttctg gtttgtgtac cggttggcgc
    13621 gacccaggtt gaacgccgcg caattcgtga atccgcgcag ggcgctggtg cccgtgaagt
    13681 cttcctgatt gaagaaccga tggctgccgc aattggtgct ggcctgccgg tttctgaagc
    13741 gaccggttct atggtggttg atatcggtgg tggtaccact gaagttgctg ttatctcctt
    13801 gaacggcgtg gtttactcct cttccgtgcg cattggtggt gatcgtttcg acgaagctat
    13861 catcaactat gtgcgtcgta attacggttc tctgatcggt gaagccaccg cagaacgtat
    13921 caagcacgaa atcggttcgg cttacccggg cgatgaagtc cgtgaaatcg aagttcgtgg
    13981 ccgtaacctg gcagaaggtg ttccacgcgg ttttaccctg aactccaacg aaatcctcga
    14041 agcactgcag gaaccgctga ccggtattgt gagcgcggta atggttgcac tggaacagtg
    14101 cccgccggaa ctggcttccg acatctccga gcgcggcatg gtgctcaccg gtggtggcgc
    14161 actgctgcgt aaccttgacc gtttgttaat ggaagaaacc ggcattccag tcgttgttgc
    14221 tgaagacccg ctgacctgtg tggcgcgcgg tggcggcaaa gcgctggaaa tgatcgacat
    14281 gcacggcggc gacctgttca gcgaagagta atcggatgca ggcaggggaa atgtctgttt
    14341 accctgcctg gtctgatacg agaatacgca taacttatga agccaatttt tagccgtggc
    14401 ccgtcgctac agattcgcct tattctggcg gtgctggtgg cgctcggcat tattattgcc
    14461 gacagccgcc tggggacgtt cagtcaaatc cgtacttata tggataccgc cgtcagtcct
    14521 ttctactttg tttccaatgc tcctcgtgaa ttgctggatg gcgtatcgca gacgctggcc
    14581 tcgcgtgacc aactagaact tgaaaaccgg gcgttacgtc aggaactgtt gctgaaaaac
    14641 agtgaactgc tgatgcttgg acaatacaaa caggagaacg cgcgtctgcg cgagctgctg
    14701 ggttccccgc tgcgtcagga tgagcagaaa atggtgactc aggttatctc cacggttaac
    14761 gatccttata gcgatcaagt tgttatcgat aaaggtagcg ttaatggcgt ttatgaaggc
    14821 cagccggtca tcagcgacaa aggtgttgtt ggtcaggtgg tggccgtcgc taaactgacc
    14881 agtcgcgtgc tgctgatttg tgatgcgacc cacgcgctgc caatccaggt gctgcgcaac
    14941 gatatccgcg taattgcagc cggtaacggt tgtacggatg atttgcagct tgagcatctg
    15001 ccagcgaata cggatattcg tgttggtgat gtgctggtga cctccggtct gggcggtcgt
    15061 ttcccggaag gctatccggt cgcggttgtc tcttccgtaa aactcgatac ccagcgcgct
    15121 tatactgtga ttcaggcgcg tccgactgca ggactgcaac gtttgcgtta tctgctgctg
    15181 ctgtggggcg cggatcgtaa cggcgctaac ccgatgacgc cggaagaggt gcatcgtgtt
    15241 gctaatgaac gtctgatgca gatgatgccg caggtattgc cttcgccaga cgcgatgggg
    15301 ccaaagttac ctgaaccggc aacggggatc gctcagccga ctccgcagca accgacgaca
    15361 ggaaatgcag ctactgcgcc tgctgcgccc acacagcctg ctgctaatcg ctctccacaa
    15421 agggctacgc cgccgcaaag tggtgctcaa ccgcctgcgc gtgcgccggg agggcaatag
    15481 tggcgagcta tcgtagccag ggacgttggg taatctggct ctctttcctc attgcgctgt
    15541 tgctgcaaat catgccctgg ccggataacc tgattgtttt ccggccaaac tgggtgttac
    15601 tcatcttgtt gtattggatc ctggccttgc ctcatcgcgt aaatgtgggc acaggttttg
    15661 tgatgggtgc catactggat ctgatcagcg gctcgacgct tggcgtacgc gtattggcga
    15721 tgagcatcat tgcttacctg gtggcgctga aataccagct tttccgcaac ctcgcattat
    15781 ggcagcaggc gctggtcgtc atgttgcttt cgctggtggt ggatattatt gttttctggg
    15841 cagagttttt agtgattaac gtctctttca gaccggaagt gttctggagt agtgtagtca
    15901 atggggtgct ctggccgtgg attttcttgc tgatgcgtaa agtccgtcag cagtttgcag
    15961 tgcaataaag gtttctatga cttctctgta tttagcttcc ggttctccgc gtcgtcagga
    16021 gttacttgcg caacttggcg tgacctttga acgtattgtt acgggcattg aggagcagcg
    16081 tcagccgcag gagagcgcgc agcagtatgt tgtgcgtctg gcgcgcgaga aagcacaggc
    16141 gggtgtcgcg caaacggcgc aggatctccc ggtgctgggt gcggatacta tcgttatcct
    16201 gaatggagaa gtgctggaga aaccgcgcga cgcagagcat gcggcgcaga tgttgcgcaa
    16261 attatcgggt cagacccatc aggtgatgac ggcagtggcg ttggccgaca gccagcacat
    16321 tctcgattgc ctggtggtca ccgatgtgac tttcagaacg ttaacagacg aagacatcgc
    16381 gggctatgtc gccagcggtg aaccgttaga taaagcaggt gcatacggta ttcaggggct
    16441 gggtggctgt tttgtcagga agataaatgg cagctatcac gccgtagtcg gcttaccgct
    16501 ggttgaaacg tatgaattat taagtaattt taacgcactg cgtgagaaaa gggataaaca
    16561 tgacggctga attgttagta aatgtaacgc cttcggaaac gcgagtggcg tatattgatg
    16621 gcggtattct gcaggaaatt catattgaac gtgaggcgcg acgcggaata gtaggcaata
    16681 tctacaaggg tcgtgtaagt cgtgtacttc cgggtatgca ggcggctttt gtagatattg
    16741 ggctggataa agccgcgttt cttcatgcat ctgacatcat gccgcacacc gaatgtgtgg
    16801 cgggtgaaga acaaaagcaa ttcacggtgc gcgacatctc ggaactggtt cgtcaggggc
    16861 aagatctgat ggtgcaggtg gtgaaagatc cgcttggcac taagggggcg cgcctgacca
    16921 ccgatatcac gctgccttct cgctatctgg tgtttatgcc aggggcttct cacgttgggg
    16981 tttcccaacg tattgaaagc gaatcagaac gtgaacgcct gaaaaaagtg gtcgcagagt
    17041 attgcgacga gcagggcggg tttatcatcc gtaccgcagc ggaaggggtt ggcgaggctg
    17101 aactggcctc cgatgccgct tatctgaaac gcgtctggac caaagttatg gagcgtaaaa
    17161 aacgcccgca gacccgttat cagctgtacg gcgaactggc gctggcgcag cgtgttctgc
    17221 gtgatttcgc cgacgccgaa ctggaccgca ttcgcgttga ctcacgcctg acttacgaag
    17281 cattgctgga gtttacctcg gagtacattc ccgagatgac cagcaagctg gagcattaca
    17341 ccggacgcca gccgattttc gatctctttg atgtcgaaaa cgaaatccag cgagcgctgg
    17401 aacgcaaagt agaactgaaa tccggtggtt atctgattat cgaccagacc gaagcgatga
    17461 ccaccgtgga catcaatacc ggagcgtttg tcggtcatcg caatctggac gacaccattt
    17521 tcaataccaa tattgaagcg acgcaggcta tcgctcgcca gttacggttg cgtaatctgg
    17581 gtgggattat cattattgat ttcatcgata tgaataatga agatcaccgc cgccgcgtgc
    17641 tgcactcgct ggagcaggcg ttgagcaaag accgggtgaa aaccagcgtt aatggttttt
    17701 cggcgctggg gctggtggag atgacgcgta aacgcacccg cgaaagcatt gagcacgtac
    17761 tgtgtaacga atgcccaacc tgccacggtc gcggaacggt gaaaaccgtg gaaacggtat
    17821 gctatgaaat catgcgcgag attgttcgtg tccaccatgc ttacgactcc gaccgtttcc
    17881 tggtctatgc ttctccggca gtagctgaag ccttgaaagg cgaagagtca cactcgctgg
    17941 cggaagtgga aattttcgtt ggcaaacagg ttaaagtaca aattgaaccg ctctataacc
    18001 aggagcagtt tgacgtcgta atgatgtaaa cagatgctgg gccgccatcc ggcaaagggt
    18061 ttttgagtca catttttagc agacaaggag tgacgggtga ggcgattgcc ggggatttta
    18121 ctgcttactg gagccgcgct cgttgtgatc gctgccctgc tggttagcgg cctgcgtatt
    18181 gctttaccgc atcttgacgc ctggcgtccg gaaatcctca acaaaataga atccgcgact
    18241 ggcatgccgg tagaagccag tcagctctca gccagctggc agaattttgg cccgacgctt
    18301 gaagcacacg acatccgtgc agaactaaaa gatggcggcg aattttcggt taaacgcgtt
    18361 actctggcgc tggatgtctg gcagagcttg ttacatatgc gctggcagtt tcgcgacctc
    18421 actttctggc agctgcgctt tcgcaccaac actcctatca ccagcggtgg tggtaatgat
    18481 agcctggaag ccagtcacat cagcgatctg tttcttcgtc aatttgacca tttcgatctc
    18541 cgcgacagtg aagtcagttt tctgacgcca tccggtcagc gcgccgagct ggcgatccca
    18601 caactcacct ggctgaacga tccacgtcga caccgtgcgg aaggcctggt aagcctctcc
    18661 agccttaccg gacagcacgg cgtgatgcag gtgcgcatgg atttgcgcga tgatgagggg
    18721 ttgttaagca atggtcgcgt ctggctacag gcggatgaca tcgacctgaa gccgtggctc
    18781 ggtaaatgga tgcaggacaa tattgctctg gaaacggcac agttctccct tgaaggctgg
    18841 atgacgatcg acaaaggcga tgtaaccggc ggtgacgtct ggctgaaaca gggcggtgcc
    18901 agctggttgg gcgagaagca aacgcatacg ctgtcggtgg ataatctgac cgcgcatatt
    18961 acgcgtgaaa atccgggctg gcagttctct attcccgata cacggatcac gatggacggc
    19021 aaaccctggc cgagcggagc attgacgctg gcctggatac cggaacagga cgttggcggc
    19081 aaagacaata aacgcagtga cgaactccgg attcgcgcca gtaatctgga gctggcaggc
    19141 ctggagggca tacgcccgct ggccgcgaaa ctttcacctg cactgggtga tgtttggcgc
    19201 tccacacaac cgagcggcaa gattaacact ctggcgctgg atatcccgct tcaggcggca
    19261 gacaagaccc gttttcaggc atcgtggagc gatctggcct ggaagcaatg gaaattatta
    19321 ccgggtgcgg aacacttctc cgggacgctt tccggcagcg ttgaaaatgg tttgcttacc
    19381 gcgtcgatga agcaggcaaa gatgccttac gaaacggtat tccgtgcgcc actggaaatc
    19441 gccgacggcc aggcaactat aagctggctg aacaatgaca aaggtttcca gctggatggg
    19501 cgtaatattg acgttaaagc caaagccgtc catgcgcgcg gcggttttcg ttacctgcaa
    19561 cctgctaacg atgaaccctg gctgggtatt ctggctggca tcagtaccga tgatggttca
    19621 caagcctggc gctatttccc ggaaaacttg atgggtaaag acctggttga ttacttaagt
    19681 ggcgcgattc agggcggtga agcggataac gcgacgctgg tttatggtgg caatccgcaa
    19741 ctcttcccct ataaacacaa cgaaggtcag tttgaagtgc tggtgccgct gcgcaacgcg
    19801 aagtttgcct tccagccgga ctggcctgca ttaactaacc ttgatattga actggacttt
    19861 attaacgacg gtttatggat gaaaaccgat ggcgttaatc tgggcggcgt gcgcgcgagt
    19921 aatctcaccg cagtgatccc tgactactca aaagaaaaac tgctgattga cgctgacatt
    19981 aaaggtccgg gtaaagccgt tggcccttac tttgatgaga caccgctgaa agattctctg
    20041 ggtgcgaccc tgcaagaact ccagctcgac ggcgatgtga atgctcgctt acatcttgat
    20101 atcccgctga acggcgaact ggtaaccgcg aaaggtgaag tgacgctgcg taataacagt
    20161 ctgtttatca aaccactcga cagcaccctg aaaaatttga gcggtaaatt cagctttatc
    20221 aatggcgatc tgcaaagtga accactgaca gcaagctggt ttaatcagcc gttgaacgtg
    20281 gatttttcca ccaaagaagg ggcaaaagcc taccaggtag cggtaaacct caacggtaac
    20341 tggcaaccgg cgaaaaccgg cgttctgcct gaagcggtga acgaagcatt gagtggcagc
    20401 gtggcgtggg atggtaaagt gggcattgag ctgccttatc atgctggcgc gacctataac
    20461 gtagagctga acggcgatct gaataatgtg agcagtcact taccttcacc gttagccaaa
    20521 cctgcgggtg aaccactagc ggtaaacgtt aaggttgatg gcaatctcaa cagctttgag
    20581 ttaaccggac aggctggtgc ggataaccat ttcaatagcc gctggttgct tggtcaaaag
    20641 ctgacgctcg atcgtgctat ttgggcggca gacagtaaaa cgctcccgcc gttgccggaa
    20701 caaagtggcg ttgaactcaa tatgccgccg atgaatggtg ccgagtggct ggccctgttc
    20761 cagaaaggcg ctgcggagag tgtcggtggg gcagcgagtt tcccacaaca cataacgtta
    20821 cgtacgccta tgttgtcact gggaaatcag caatggaata acctgagtat tgtttcgcaa
    20881 ccgacggcaa atggcaccct ggttgaagcg caggggcgtg aaatcaatgc cacgctggcg
    20941 atgcgtaata acgcgccgtg gctggcgaat atcaaatatc tttattacaa cccgagcgtg
    21001 gcgaaaactc gtggtgattc aacgccgtca tcacctttcc cgacaacgga gcgcattaac
    21061 ttccgtggct ggccggacgc acaaatacga tgcgcagagt gctggttctg ggggcaaaaa
    21121 ttcggtcgca ttgacagtga tctcaccatt tctggcgata cgttaacgct gaccaatgga
    21181 ctgattgata ctggtttctc gcggcttact gccgatggtg aatgggttaa taatctgggg
    21241 aatgaacgta cctcgctgaa aggaaaactg cgcgggcaga aaattgatgc cgccgcagaa
    21301 ttttttggtg tcacgacgcc aatacgccag tcgtcattta atgtggatta cgatttacac
    21361 tggcgcaaag caccctggca gccagatgaa gcgacgttga atggcatcat tcatactcaa
    21421 ctgggtaaag gcgaaattac cgaaatcaat accggacatg ccgggcaatt gctgcgctta
    21481 ttgagcgtag atgccctgat gcgtaagctg cgttttgatt tcagagacac ttttggcgaa
    21541 gggttctatt ttgactccat tcgcagcacc gcgtggatta aagacggcgt tatgcacacc
    21601 gacgacacgc tggtggatgg cctggaagcg gacatcgcta tgaaagggtc ggtaaatctg
    21661 gtacgtcgcg acctgaatat ggaagcggtt gttgcaccag agatttctgc gacggtgggc
    21721 gtggctgcgg cttttgcggt taaccccatt gttggcgcgg cagtgtttgc cgccagtaaa
    21781 gtgctggggc cgctgtggag caaagtctcc attttgcgct atcacatttc gggtccgctg
    21841 gacgatccgc aaatcaacga agtgttgcgc caaccgcgta aagaaaaagc gcaatgattt
    21901 gacgagggcg cgtaattgcc ccaatctcat atgataatcg ttgccaaagg ccaacgagcc
    21961 agaacataac cgtaggccgg acagggcgtt cacgccgcat ccggcagccg taaaaatcct
    22021 ctactgcagt aactaacgag tagcaaaaac gatgagtctt aacctggtaa gtgaacaatt
    22081 gctagcggcg aacggcctga aacatcagga cttgttcgcg atcctcggtc aactggccga
    22141 acgtcgcctt gattatggcg atctctattt tcagtcgagc tatcacgaat cctgggtttt
    22201 agaagaccgc attattaaag atggttctta caacatcgat cagggcgttg gcgtgcgtgc
    22261 aatcagcggt gaaaaaaccg gatttgctta cgctgaccaa atcagcctgc tggcgctgga
    22321 acagagtgcg caagcggcgc gcaccatcgt ccgtgatagt ggtgatggca aagtacagac
    22381 gctgggcgcg gtagagcata gcccgttgta tacctcggta gatccgctgc aaagcatgag
    22441 ccgtgaagag aagctggata tcctgcgtcg cgtcgataag gttgctcgcg aagcggacaa
    22501 gcgcgtacag gaagtgactg ccagcctcag tggtgtctat gaattaattc tggttgcggc
    22561 caccgacggc acgctagcgg cggatgtccg tccgctggtg cgtctttccg tgagcgttct
    22621 ggtcgaagaa gatggcaaac gcgaacgcgg tgccagtggc ggcggcggtc gttttggtta
    22681 tgagttcttc cttgccgatc ttgacggcga agtccgtgcg gatgcatggg caaaagaagc
    22741 agtgcgtatg gcgctggtca atctttctgc cgttgctgca ccagcgggca ccatgccggt
    22801 agtacttggc gcaggttggc cgggcgtgct gttgcatgaa gcggtaggtc acggtctgga
    22861 aggcgacttc aaccgccgcg gcacttcagt atttagtggg caggtcgggg agctggtggc
    22921 ttcagaactg tgtaccgtgg ttgatgacgg cacgatggtc gatcgccgtg gttcggtggc
    22981 gattgatgac gaaggtacgc caggccagta caacgtgctg attgagaacg gcattctgaa
    23041 aggctacatg caggataaac tcaacgcgcg tttgatgggg atgacgccga ctggcaacgg
    23101 tcgccgtgaa tcctacgccc atctgcccat gccgcgtatg accaacacct atatgctgcc
    23161 gggtaaatcg accccgcagg aaattattga atccgttgag tacggtatct atgcaccgaa
    23221 ctttggtggc ggtcaggtgg atatcacctc cggcaaattc gttttctcca cttcagaagc
    23281 atatctgatt gaaaacggta aagtaacgaa gccggtgaaa ggcgcaacgt tgattggttc
    23341 cggtatcgaa accatgcagc agatttcgat ggttggcaac gacctgaaac tggataacgg
    23401 cgtgggtgtc tgcggtaaag aagggcaaag tttgccggtt ggcgtgggcc agccaacgct
    23461 gaaagttgat aacctgactg ttggcggtac tgcgtaataa tttcattatt ttcagaagga
    23521 taattaatct ttctacgtgc acgaacggtc ccctcgctcc tctggggtta gagtgagggg
    23581 aacccgttgg cacaggtttg tacaacgtaa cagtacaata tgaattactt ctctttcccg
    23641 cgcccgtgca tctcctgaaa caatttaccg acctcaacaa aataatccgt cagcgagttg
    23701 atcacgacct gtaccttcag cggcagctta tctttttcgg tatataacgc ataaaccggg
    23761 cgtggatctg actgataacg cggcagcagg atctccagct ccccacgatt gatctcgttg
    23821 atcacccaca tcagcggcac gtaggcgatc ccggcacccg ccgttagcca gcgcaccagc
    23881 gtcatcggat cattagtcac aaatctccct tgcgggatca ggcgagtcga gatcccttcc
    23941 ggtgcaatca gttcaaattc attgtcgggc cgcacgctgt attcaagcca tgaatggcta
    24001 cttaaatcgg cgggtttttc cggtatgccg tattgtgtga gatagctttt cgcggcgcac
    24061 accaccattg gcatcgcgcc aagacggcgg gaaaacaggc tggaatcctg caacgcgccg
    24121 acgcggatca ccacatccag accgtcggca atcaggtcgg gggctggaat tccggtaacc
    24181 agattgacgc tcaaacctgg gtattctttc agcattttgg ctgtcagccc ggcgagaaca
    24241 ttttgtgcca tagttgaaga acagccaatg cgtagcgtcc cgatgggggt gttattgaag
    24301 gcatacagtt gctcatgaac atcctgcact tcatgaagca tacgacggca gccctggtag
    24361 taaattctac cggcttcggt caggccaatg ctgcgtgtgc tacggtttaa cagctttacc
    24421 tgcaactcat cttccagttt tgacaccgtc tgactgatgg acgaaacgct catctgtagc
    24481 tgtctggcgg cggcggtaaa agagccaaat tcaactactt tggcaaacac cgacatgcgt
    24541 tttagtcgtt ccattattca ctctgactta aaagtgattt agatcacata atatagataa
    24601 cagcataaca gttacgctaa tatattaaat atcaacctac agcaatgttg ctctcgcccg
    24661 gctttccatg cccttctctg cggcgacaga tgctgaaaat aataacgcct gctctctcta
    24721 tacaaccaag gtcaacatga gtctgtttcc cgttatcgtg gtgtttgggc tgtccttccc
    24781 accgatattt tttgaattgc ttttatcact ggcgattttc tggctggtgc gccgggtact
    24841 tgtgccaaca ggtatctacg actttgtctg gcatccggcg ttgttcaaca ccgcgctcta
    24901 ttgctgcttg ttttatttga tatcgcgact gttcgtttga ggttgaagtg aaaacactaa
    24961 taagaaaatt ctcccgtacg gccatcacgg tcgtattagt cattctggcc ttcatcgcaa
    25021 tttttaatgc ctgggtctat tacaccgaat ccccctggac gcgtgacgcg cgctttagcg
    25081 ctgacgtcgt tgcgatcgcg ccggacgttt ctggactcat tacccaggtg aatgttcatg
    25141 ataaccagct ggtgaaaaaa ggacagatac tgttcaccat cgaccagccg cgctatcaaa
    25201 aggcgcttga ggaagcgcaa gccgatgttg cttattatca ggtactggca caggagaaac
    25261 gccaggaggc cggacgtcgt aaccgtctcg gtgtgcaggc gatgtctcgc gaagagatcg
    25321 accaggctaa caacgtacta caaacggttc tgcatcagtt agcgaaagcg caggcgaccc
    25381 gcgatctggc aaaactggat cttgaacgca cggtgatccg cgcgccagca gatggctggg
    25441 tgaccaacct caacgtctat accggtgagt ttattactcg aggatcaacg gcggttgcgc
    25501 tggtgaaaca gaactccttc tatgtactgg cctatatgga agaaactaag ctggaagggg
    25561 tgcgtccggg gtatcgtgca gagatcacgc cgcttggcag taacaaagtg ctgaaaggga
    25621 ctgttgatag tgttgccgca ggggtcacca acgccagcag cacgcgtgac gacaaaggga
    25681 tggcgactat agactctaac cttgaatggg tgcgtcttgc gcaacgtgtt ccggttcgta
    25741 ttcgtctcga caaccagcaa gagaacatct ggcctgcggg caccactgct acagtggtgg
    25801 tcactggcaa acaagatcgc gacgaaagcc aggattcgtt cttccgtaaa atggcccatc
    25861 gcctgcgtga gtttggttaa tcacgatggg tattttctcc attgctaacc aacatattcg
    25921 ctttgcggta aaactggcga ccgccattgt actggcgctg tttgttggct ttcacttcca
    25981 gctggaaacg ccacgctggg cggtactgac agcggcgatt gttgccgccg gtccggcctt
    26041 tgctgcggga ggtgaaccgt attctggcgc tattcgctat cgtggctttt tgcgcatcat
    26101 cggcacattt attggctgta ttgccggact ggtgatcatc attgcgatga tccgcgcacc
    26161 attattgatg attctggtgt gctgtatctg ggcaggtttt tgtacctgga tatcctcgct
    26221 ggtacgaata gaaaactcgt acgcgtgggg gctggccggt tataccgcgc tgatcattgt
    26281 gatcaccatt cagccggaac cattgcttac gccgcagttt gccgtcgaac gttgtagcga
    26341 gatcgttatc ggtattgtgt gtgcgattat ggcggatttg ctcttttctc cgcgatcgat
    26401 caaacaagaa gtagatcgag agctggaaag tttgctggtc gcgcaatatc aattaatgca
    26461 actctgtatc aagcatggcg atggtgaagt tgtcgataaa gcctggggcg acctggtgcg
    26521 acgcaccacg gcactacaag gcatgcgcag caacctgaat atggaatctt cccgctgggc
    26581 gcgggccaat cgacgtttaa aagcgatcaa tacgctatcg ctgacgctga ttacccaatc
    26641 ctgcgaaact tatcttattc agaatacgcg cccggaattg atcactgata ctttccgcga
    26701 attttttgac acgccggtag aaaccgcgca ggacgtccac aagcagctca aacgcctgcg
    26761 gagagttatc gcctggaccg gggaacggga aacgcctgtc accatttata gctgggtcgc
    26821 ggcggcaacg cgttatcagc ttctcaagcg cggcgttatc agtaacacaa aaatcaacgc
    26881 caccgaagaa gagatcctgc aaggcgaacc ggaagtcaaa gtagagtcag ccgaacgtca
    26941 tcatgcaatg gttaacttct ggcgaaccac actttcctgc attctgggcg cgcttttctg
    27001 gctgtggacg ggctggactt ccggcagtgg tgcaatggtg atgattgcgg tagtgacgtc
    27061 actggcaatg cgtttgccga atccacgcat ggtggcgatc gactttatct acgggacgct
    27121 ggcagcgctg ccgttagggc tgctttactt tttggtgatt atccctaata cccaacagag
    27181 catgttgctg ctgtgtatta gcctggcagt gctgggattc ttcctcggta tagaagtaca
    27241 gaaacggcga ctgggctcga tgggggcact ggccagcacc ataaatatta tcgtgctgga
    27301 taacccgatg actttccatt tcagtcagtt tctcgacagc gcattagggc aaatcgtcgg
    27361 ctgtgtgctc gcgttcaccg ttattttgct ggtgcgggat aaatcgcgcg acaggactgg
    27421 acgtgtactg cttaatcagt ttgtttctgc cgctgtttcc gcgatgacta ccaatgtggc
    27481 acgtcgtaaa gagaaccacc tcccggcact ttatcagcag ctgtttttgc tgatgaataa
    27541 gttcccaggg gatttgccga aatttcgcct ggcgctgacg atgattatcg cgcaccagcg
    27601 cctgcgtgat gcgccgatcc cggttaacga ggatttatcg gcgtttcacc gacaaatgcg
    27661 ccgcacagca gaccatgtga tatctgcccg tagcgatgat aaacgtcgtc ggtactttgg
    27721 ccagttgctg gaagaactgg aaatctacca ggaaaagcta cgcatctggc aagcgccacc
    27781 gcaggtgacg gaaccggttc atcggctggc ggggatgctc cataagtatc aacatgcgtt
    27841 gaccgatagt taagtcaaaa ccgacgccaa aagcgtcggt tttttcatgg ctatacttag
    27901 cgatgcacgg cagaactcgc cgcgaaacgt gacggtggca acagatgaat atttatacct
    27961 ttgattttga tgagattgag agtcaggagg atttttatcg tgactttagc caaacctttg
    28021 gtctggctaa agataaggta cgcgatctcg actcactatg ggatgtgtta atgaacgatg
    28081 tcctgccgct accacttgag attgaatttg ttcatctggg agagaaaacg cgtcgccgtt
    28141 ttggcgcgtt aatattgctg tttgatgagg cagaggaaga gctggaaggg catttgcgtt
    28201 ttaatgttcg tcattagcgc aaaaaaaagc ccccgaaccg ggggcaatat cgtcggacaa
    28261 gacgatgagg gtttatttgt acagttcagc cgtagcgtgc caggtgtcac cgctacgagc
    28321 ttcagtaatc tggtaggccg ttgcgccttt ctcttccgct tttttgttca gcatttcacg
    28381 catatccatt ggcgaagacg ccacaccact tacggatacg gtcccgattg cttcacgatt
    28441 ttgtgcttgt gcagcatcaa tggagtcggc agcgaatgca ccgaaagaga gaacagaaag
    28501 tacgcttaat gcagctacag tggttttgat tttcatgatt tttacctcga cataatcttt
    28561 tagctgggcc tttgtttcgt gacccatatc acaaaatcaa gtatacacta attattgaac
    28621 taattaatac cacactaact gtttcagttg atactaagtg ttaaaaatgt gaattttaat
    28681 aacaaaaaag tataaaaaca cggttattta ttattaatta tagtattttc aattagataa
    28741 tgaattttgc ataatgtgct ttttttaccg tatgtattca ttttgtgaat gatgtgtcgc
    28801 ggtaaaacgc actattcgtt aaatgtatcg caggagaaag aaggggaatg agagggataa
    28861 gcaacatttt ccccgccgcc agaagcgacg gggcagagat taaagctcct ggtcgaacag
    28921 ctctaaaatc gcttcgtaca ggtctttgac ggtgaaaccg ttagcagggg tggtaaagat
    28981 ggtgtcatcg ccagcgatgg tgcccagaat accttctgct ttgcccagtg agtccagcag
    29041 gcgagcaatt aactgcgccg cgccagggct ggtatgaatc acgacaactg catcgttgta
    29101 gtcgatatcc agcaccagat tcttcaatgg actggaggta gttggtacac ccagttcagc
    29161 tggcaggcag taaaccattt ccattttggc attgcgtgta cgtacagcac caaacttggt
    29221 caacatccgc gagactttag actgattaat attgtcaaag ccttgctcct gcaacgcggc
    29281 gacgatttcg ccctgggagc taaatttctc ttctttaagt aatgctttaa atgctttaac
    29341 tagttcttct tgcttagccg agcttcgcat aagtcacccg atatggtggt tgatacaaca
    29401 ttattgtgca tacagatgaa tttttatgca aacagtcagc cctgaagaag gctgaaataa
    29461 tgttatgaaa gagcgggatt ttatcaaatt tcgttattga gaaacatgcc tgcgtcacgg
    29521 catgcaaatt ctgcttaaaa gtaaattaat tgttatcaaa ttgatgttgt tttggctgaa
    29581 cggtagggta tattgtcacc acctgttgga atgttgcgct aatgcataag cgactgttaa
    29641 ttacgtaagt taggttcctg attacggcaa ttaaatgcat aaacgctaaa cttgcgtgac
    29701 tacacattct tgagatgtgg tcattgtaaa cggcaatttt gtggattaag gtcgcggcag
    29761 cggagcaaca tatcttagtt tatcaatata ataaggagtt taggatgaaa gtcgcagtcc
    29821 tcggcgctgc tggcggtatt ggccaggcgc ttgcactact gttaaaaacc caactgcctt
    29881 caggttcaga actctctctg tatgatatcg ctccagtgac tcccggtgtg gctgtcgatc
    29941 tgagccatat ccctactgct gtgaaaatca aaggtttttc tggtgaagat gcgactccgg
    30001 cgctggaagg cgcagatgtc gttcttatct ctgcaggtgt agcgcgtaaa ccgggtatgg
    30061 atcgttccga cctgtttaac gttaacgccg gcatcgtgaa aaacctggta cagcaagttg
    30121 cgaaaacctg cccgaaagcg tgcattggta ttatcactaa cccggttaac accacagttg
    30181 cgattgctgc tgaagtgctg aaaaaagccg gtgtttatga caaaaacaaa ctgttcggcg
    30241 ttaccacgct ggatatcatt cgttccaaca cttttgttgc ggaactgaaa ggcaaacagc
    30301 caggcgaagt tgaagtgccg gttattggcg gtcactctgg tgttaccatt ctgccgctgc
    30361 tgtcacaggt tcctggcgtt agttttaccg agcaggaagt ggctgatctg accaaacgta
    30421 tccagaacgc gggtactgag gtggttgaag cgaaagccgg tggcgggtct gcaaccctgt
    30481 ctatgggcca ggcagctgca cgttttggtc tgtctctggt tcgtgcactg cagggcgaac
    30541 aaggcgttgt cgaatgtgcc tacgttgaag gcgacggtca gtacgcccgt ttcttctctc
    30601 aaccgctgct gctgggtaaa aacggcgtgg aagagcgtaa atctatcggt accctgagcg
    30661 catttgaaca gaacgcgctg gaaggtatgc tggatacgct caagaaagat atcgccctgg
    30721 gcgaagagtt cgttaataag taattgatta gcgaataata aaaaaccgga gcacagactc
    30781 cggtttttgt tttgagcact cgacttaatt ggttgccgga tattcctgaa tggtgacctg
    30841 cagcgttaac tgcttatcat cacgcatcac tacaaccggg atcaccgaac cagggcgaat
    30901 ttccgccacc tgatccatcg tctccagagc agagatggcc ggtttgttat ccaccgaaat
    30961 aatcagatcg ttgacctgaa tacccgcatt cgccgccggg ccgtcaggtg acacttcatt
    31021 aaccacgatc ccttgcagtt gatctatacc accgccctgc gcgtgcagtg gtgcgatctc
    31081 gcgtccgccg ataccaatgt agccgcggat cacgcgacca tcgcggatca gcttatccat
    31141 aattttggtt gctaactgga aaggaatcgc aaagccgata ccttccggcg tttcgccatc
    31201 gttactctta tcaaacgaca gcgtgttaat gcccatcagt tctcccagcg agttcaccag
    31261 cgcgccgcca gagttaccgt ggttaatgga agcatcggtt tgtaggaagt tttgccgccc
    31321 ggtcgggttc agaccgattc gacccgtggc actaataatc ccctgggtaa tggtctgccc
    31381 gaggttgtac gggttaccga tcgccagtac tacgtcgcca atgtgcggta cgcgacgtgc
    31441 attaattgga atggtaggta aaccgccagt ggcattaatt ttaagtaccg ccagatcggt
    31501 tagagagtca gatcccacca gcaatgcttc aaatacacgt ccatcctgta aggcgacgat
    31561 gatctgatcg gcgtcgttga tgacgtgttt attggtgatg atataaccgc gttgatccat
    31621 gattacaccg gatcccaggg tgcggatctc aagctggttg tgagagttgg tgttcaaacc
    31681 acggttgtaa acgttaacca ccgctggcgc ggcgcggcga accgccagat tatagctggc
    31741 aggcgtctca tcggtactgt caaattgcgg agtggaaagc gggttaaggc tgcgcagcga
    31801 aggcatggca accagcagaa tagcgccgac aattaatcca atcgcaacgg aacgtaagag
    31861 cttcacaaac atgatggagg cgtcattaaa aaagggaacg gcagcagcat accacgagtt
    31921 aaccggacat cacacgtcag cctgatgccc ggtttacgac attaacgcat cagcagatag
    31981 atgctttcat tgccgcgtac aatttgcagg gcaatgatgg ccggttttgc cgccagcact
    32041 ttacgcattt cagcaatcga gctcacccga tcgcggttga cgccaatgat cacatcgtct
    32101 ttttgcaagc cagcctgagc agctgggctt cctttgacaa cttcatctat cttaatacct
    32161 ttgccgccat cttttagctg accatcgctc aacgttgcac cttccagcgc tggcgtgatc
    32221 atttcagcgc tggccgacga agaggtgctg gtatcaagcg tcacttctac ttccagtggt
    32281 ttgccgttac gcagcaggcc aagcttcact ttcgtgcccg gctctgtagt cgcgatacga
    32341 gagcgcaact cagcaaagct attcagcggt ttgccgttga ggctggtaat aatatcgccc
    32401 gctttgacgc ccgcttttgc cgagccagaa cctggcaaca cttcgctgac aaacgcgcca
    32461 cgctgcacgt caaggttgaa ggctttggcg atatcggcac tcatctcggt gcctttgatg
    32521 cctaacaaac cgcgtttgat ttcaccaaag tcgataagct gctgcgccag tgttcgcgcc
    32581 atattactgg ggatggcaaa tccaatcccg acgctcccgc cgccaggcgc aaggattgca
    32641 gtgttgatgc caattaactc accgttaagg tttaacagtg caccgccgga gttaccgcgg
    32701 ttaatggaag catctgtctg gataaagttt tccagacctt caagattcaa cccactgcgg
    32761 cctaatgcgg aaacaatgcc agaggtggcg gtttgcccaa ggccaaatgg gttaccgacc
    32821 gctacggcaa aatcaccgac gcgcaattta tcggagtcgg caatagcgat ttgcgttaat
    32881 ttgctcgggt tttgaatttg taacagggcg atatcgctct ggtcatcgct accaatcagt
    32941 tttgcatcaa actcgcgccc atcattgagc tgaatactga ttttctgtgc ctgattaatc
    33001 acatggttgt tggtcagcac atagccttta ctggcgttga tgatgacacc ggaacctaaa
    33061 ccttcgaagg gttgtgcagg ttgatccggt aaatcatcac caaaaaactt tttgaattct
    33121 tccgggattt tctgtccctg actggccgtt ccttccaccc gtacgctcac cactgccgga
    33181 agcacttttt ccagcattgg agccagactg gggagagggg cctgatcggc aacttggcct
    33241 ggaatcgacg cgacggcctg aaatgacgcc gagagagtta acccgacact taacgctaat
    33301 gcactcaaca gctgggtttg ttttttcatt attcctgctc tcgtacctga atgataagaa
    33361 aagagattca aaacagtttg atgttattga attttcagac ggctaacaat gagacaccag
    33421 attaaatgat agtcggggag ggagagaaga ggagggcgca atggctgcgc ccgaaaaata
    33481 aattagtcgc gcttcgcgcc agtacgcagc aggccggatg cgccttcaga atagtcgcga
    33541 ggcatctgca ccggtgcctg atcgttgctg gcttcagact ctgccagacg attacggaac
    33601 gggtttgctt cagcagacag ttccggcagc aggctgctgg agcttttcgc catgtgctga
    33661 tacagctggc gatagtcgtg cgccatggta tccagtaatt ccgcgctgcg ggcaaagtgg
    33721 ctaaccagct cttcgcgata ctcgtccagt tcagctttat tcttttccag ttcgtactgc
    33781 aacgcctgtt gctggcgtag tttacgatta ccaaaacgca tggccacagc accaataatg
    33841 atgccgacga ctaacccaat tagcgcatat tcccaggtca tgaacatctc ccgttgtctt
    33901 ttgtttccgt agggtgttgg cttcaggctc catgcctgcg gctgattatg ccactataac
    33961 cgttaattcc acagaagtgg aatcccgact gcatatcgcg tagtgtagaa cggccttttt
    34021 ttcgtcaacc gtgaacaacg gcgcaccgat tattcaagga ataacaataa gatcatgcaa
    34081 agcgttaccc caacatcgca atacctgaag gcgctcaatg aaggcagcca tcaacccgac
    34141 gacgttcaaa aagaggccgt cagccgcctg gaaattattt atcaagaact catcaatagc
    34201 aggccaccag cccccaggac gagtgggcta atggcgcggg tcggtaagct gtggggtaaa
    34261 cgcgaagaca caaagcatac gccagtgcgt ggcttatata tgtggggcgg tgtaggacgc
    34321 gggaaaacct ggctgatgga ccttttctat caaagcctgc cgggagagcg gaaacagcgc
    34381 ctgcactttc accgttttat gctgcgggtg cacgaagagc taactgcctt acaggggcag
    34441 accgatccgc tggaaattat tgccgatcgc tttaaagccg aaactgacgt gctctgtttt
    34501 gacgaatttt ttgtttctga tattaccgac gccatgctac ttggcggtct gatgaaagcc
    34561 ctgttcgccc gcggtattac cctggtagcg acgtcaaata ttccgccgga cgaactttat
    34621 cgaaatggct tgcaacgtgc gcgttttctg cctgcaatcg atgccattaa acagcattgt
    34681 gatgtaatga acgtggacgc tggtgttgat tatcgacggc gtacactcac tcaggcgcat
    34741 ctgtggcttt cgccactcca cgatgaaacc cgggcacaaa tggataaact atggttggcg
    34801 ctggcgggag cgaaacgcga aaattcaccg acgttagaaa tcaaccatcg accgctggcg
    34861 acaatgggcg ctgagaatca gacgcttgct gtctctttta ccaccttgtg tgtcgatgcc
    34921 cgcagccagc atgactatat tgcgctctca cgcctctttc atacggtcat gttgtttgat
    34981 gtaccagtta tgacgcggtt gatggagagc gaagcgcggc gctttattgc gctggtggat
    35041 gagttttacg agcgccatgt caaattagtg gtgagtgcag aagtgccgct gtatgaaatt
    35101 tatcagggcg atcggctgaa gtttgagttc cagcgttgcc tgtcacgtct gcaagagatg
    35161 caaagcgaag agtatctgaa gcgcgagcat ttggcgggtt aaaacctgtc acaaatcaca
    35221 aaaaggggtc gatctttgac cccgacttct ctataatcct gcgaccccac gttacaagaa
    35281 agtttttttc ccaaaacttt ttgtgtgctg gcataggcta ttcgaagggg taggtttgcc
    35341 ggactttgtc gtgtgaacct caacaattga agacgtttgg gtgttcacca acgtgtaact
    35401 atttattggg taagctttta atgaaaactt ttacagctaa accagaaacc gtaaaacgcg
    35461 actggtatgt tgttgacgcg accggtaaaa ctctgggccg tctggctact gaactggctc
    35521 gtcgcctgcg cggtaagcac aaagcggaat acactccgca cgtagatacc ggtgattaca
    35581 tcatcgttct gaacgctgac aaagttgctg taaccggcaa caagcgtact gacaaagtgt
    35641 actatcacca caccggccac atcggtggta tcaaacaagc gacctttgaa gagatgattg
    35701 ctcgccgtcc tgagcgtgtg attgaaatcg cggttaaagg catgttgcca aaaggcccgc
    35761 tgggtcgtgc tatgttccgt aaactgaaag tttacgcggg taacgagcac aaccacgcgg
    35821 cacagcaacc gcaagttctt gacatctaat cgggattata ggcaatggct gaaaatcaat
    35881 actacggcac tggtcgccgc aaaagttccg cagctcgcgt tttcatcaaa ccgggcaacg
    35941 gtaaaatcgt aatcaaccaa cgttctctgg aacagtactt cggtcgtgaa actgcccgca
    36001 tggtagttcg tcagccgctg gaactggtcg acatggttga gaaactggac ctgtacatca
    36061 ccgttaaagg tggtggtatc tctggtcagg ctggtgcgat ccgtcacggt atcacccgcg
    36121 ctctgatgga atacgacgag tccctgcgtt ctgaactgcg taaagctggc ttcgttactc
    36181 gtgacgctcg tcaggttgaa cgtaagaaag tcggtctgcg taaagcacgt cgtcgtccgc
    36241 agttctccaa acgttaattg gcttctgctc cggcagaaaa caatttgcga aaaacccgct
    36301 tcggcgggtt tttttatggc taaattttga atcagcgtaa aaaatggaaa gttgcttttt
    36361 gctaccgtct gacagacagg caaaacaaaa accacatcgc caataagggg ctaagtcaac
    36421 tatttcagac taaagcgcat ctctttttcc ccatttccgg catcgactca ccacaaaggt
    36481 cgcaaaatct ggtaaactat catccaattt tctgcccaaa tgtcgggtat tgctcatttt
    36541 ttgtttgatt ttcgaacaaa gagagtcgtt ccttactggg taacacaact tctgactggc
    36601 cacctggtgg ctggtagcag taaaaattct gactatacct ggaggttttc atggctgtcg
    36661 ctgccaacaa acgttcggta atgacgctgt tttccggtcc tactgacatc tatagccatc
    36721 aggtccgcat tgtgctggct gagaaaggtg taagtttcga gatcgaacac gtggaaaagg
    36781 acaatccgcc tcaggatctg attgacctca acccgaatca gagcgttccg accctggtgg
    36841 atcgtgagct gaccctgtgg gaatctcgca tcattatgga atatctggat gagcgtttcc
    36901 cgcatccgcc actgatgcct gtttacccgg tagctcgcgg tgaaagccgt ctgtacatgc
    36961 atcgcatcga aaaagactgg tacacgctga tgaacaccat catcaacggt tcagcttctg
    37021 aagcagatgc cgcacgtaag caactgcgcg aagaactgct ggcgattgcg ccggtcttcg
    37081 gtcagaagcc gtacttcctg agcgatgagt tcagcctggt cgattgctat cttgctccgc
    37141 tgctgtggcg tctgccgcaa ctgggcatcg agttcagcgg cccgggtgcg aaagagctga
    37201 aaggctatat gacccgcgtc tttgagcgtg actctttcct tgcttcttta actgaagcag
    37261 aacgtgaaat gcgtctgggc cggagttaat ctgtatggat ttgtcacagc taacaccacg
    37321 tcgtccctat ctgctgcgtg cattctatga gtggttgctg gataaccagc tcacgccgca
    37381 cctggtggtg gatgtgacgc tccctggcgt gcaggttcct atggaatatg cgcgtgacgg
    37441 gcaaatcgta ctcaacattg cgccgcgtgc tgtcggcaat ctggaactgg cgaatgatga
    37501 ggtgcgcttt aacgcgcgct ttggcggcat tccgcgtcag gtttctgtgc cgctggctgc
    37561 cgtgctggct atctacgccc gtgaaaatgg cgcaggcaca atgtttgagc ctgaagctgc
    37621 ctacgatgaa gataccagca tcatgaatga tgaagaggca tcggcagaca acgaaaccgt
    37681 tatgtcggtt attgatggcg acaagccaga tcacgatgat gacactcatc ctgacgatga
    37741 acctccgcag ccaccacgcg gtggtcgacc ggcattacgt gttgtgaagt aatacaaaaa
    37801 caggccctgg cggcctgttt tgtcttttta atggcttaat aaaatagcgt gattgtggca
    37861 atcatattca ccacgaaacc gactgccatg gggattgctg tccgcttcac caccgcaaaa
    37921 ggcgaaacgc ctgcaattcc tgacgttacg accaccaccg cagtaatcgg cgaaaccgcg
    37981 cgcgctagcg tcgtggcaaa atgcatcggc ataatcatta caaccgctgg tacatgtagt
    38041 ccggctgcga tattcggaat aagactggca aatgacataa acggcgcatt gcctgagccc
    38101 atcacaatgg cacaaatagc aatgactagc gccataataa tcatcacgcc aataccgccc
    38161 agaccagaat gctccgcacc cctgataacc gcatcaacag tgccaatcgt cgttaagcct
    38221 ttcgcaaata tttcacccgc gaccaccagc gttaccacgt tggcaaactg cgtacccatg
    38281 ccgtcaaaaa acgcctgcac atcgtccatt gtttcgcgca agttatgctt gcggaagaac
    38341 tcaacataca tcgtcaccgt caaacttagt aacatcacca ccaccagatg cagttctgac
    38401 ttcatcagcc cgacgtgggc gaggaacagc gagccgagca tcaggattaa cggcatcaca
    38461 ggtaaaatgg cgtaatagag cggcgggaca ttatcgagag ttttttgctc tgcctgttcg
    38521 tgattgatat ttttatcttt tttgtcaaaa gcgcgttgca cgaaaaagtg ggagatcgcc
    38581 accgagataa tgacgcaaga ggcgaccgga agctggtagt ggaagaagta tgtggcaatt
    38641 ttcattcccg ctacctgggc tgcaaaaatg gagttcgttt ccagaatccc ccactcaatg
    38701 gacatcgtgg ttgcgataac cgccaccgca gagaggcgac ttactcccag actcaccagc
    38761 gtcggaaata aggtgaccat cagcaacata cccagaccgg aggcgctggt aataaactgc
    38821 gccatgattt ggccgatgac gtaagttgcc gacagaataa tatacggcga gcgaatgagt
    38881 tttaacgggc ggcttagcaa gctcaccatc gcgcgactgg cccctgtgcg ctccatgtag
    38941 cgggcataac cgcccaccgc cataatcgac agccccaatc cggcaatgcg gttggacagc
    39001 atgcgcagga tttcattata aatatcgacg atcaagtagc cgctgctttt ggcgggatcg
    39061 agtacaccac cgaaccctaa ccagacaccg cacatcatca ggaagatacc tgcggcagcc
    39121 agcaccacct gaggtttgta gtttttcagg atcaaatagc ccatcgtaat taatacgatg
    39181 acagatataa ttatgccgaa cattgttgaa ctccgtgtca aaagaaaacg gtcaatccca
    39241 taaacggcag attgaaaaca atgatgttat attttttgca agtctattta tggtgcggat
//
LOCUS       DAZKHC020000038        37380 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZKHC020000038 DAZKHC020000000
VERSION     DAZKHC020000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 37380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.038"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..304)
                     /locus_tag="ACHOSM_RS11205"
                     /old_locus_tag="pgaptmp_002241"
     CDS             complement(<1..304)
                     /locus_tag="ACHOSM_RS11205"
                     /old_locus_tag="pgaptmp_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4558756.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSELMK"
     gene            complement(427..879)
                     /gene="tabA"
                     /locus_tag="ACHOSM_RS11210"
                     /old_locus_tag="pgaptmp_002242"
     CDS             complement(427..879)
                     /gene="tabA"
                     /locus_tag="ACHOSM_RS11210"
                     /old_locus_tag="pgaptmp_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HGU4558757.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(1024..1617)
                     /gene="bdcR"
                     /locus_tag="ACHOSM_RS11215"
                     /old_locus_tag="pgaptmp_002243"
     CDS             complement(1024..1617)
                     /gene="bdcR"
                     /locus_tag="ACHOSM_RS11215"
                     /old_locus_tag="pgaptmp_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU4558758.1"
                     /translation="MITKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAELTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCASAALAGEAIKTLLKE"
     gene            1688..2401
                     /gene="bdcA"
                     /locus_tag="ACHOSM_RS11220"
                     /old_locus_tag="pgaptmp_002244"
     CDS             1688..2401
                     /gene="bdcA"
                     /locus_tag="ACHOSM_RS11220"
                     /old_locus_tag="pgaptmp_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4558759.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNANDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2532..2927
                     /gene="yjgH"
                     /locus_tag="ACHOSM_RS11225"
                     /old_locus_tag="pgaptmp_002245"
     CDS             2532..2927
                     /gene="yjgH"
                     /locus_tag="ACHOSM_RS11225"
                     /old_locus_tag="pgaptmp_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU4558760.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGS
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            3208..3342
                     /gene="pyrL"
                     /locus_tag="ACHOSM_RS11230"
                     /old_locus_tag="pgaptmp_002246"
     CDS             3208..3342
                     /gene="pyrL"
                     /locus_tag="ACHOSM_RS11230"
                     /old_locus_tag="pgaptmp_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HGU4558761.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3346..4281
                     /gene="pyrB"
                     /locus_tag="ACHOSM_RS11235"
                     /old_locus_tag="pgaptmp_002247"
     CDS             3346..4281
                     /gene="pyrB"
                     /locus_tag="ACHOSM_RS11235"
                     /old_locus_tag="pgaptmp_002247"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HGU4558762.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4294..4755
                     /gene="pyrI"
                     /locus_tag="ACHOSM_RS11240"
                     /old_locus_tag="pgaptmp_002248"
     CDS             4294..4755
                     /gene="pyrI"
                     /locus_tag="ACHOSM_RS11240"
                     /old_locus_tag="pgaptmp_002248"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HGU4558763.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4828..5214
                     /gene="ridA"
                     /locus_tag="ACHOSM_RS11245"
                     /old_locus_tag="pgaptmp_002249"
     CDS             4828..5214
                     /gene="ridA"
                     /locus_tag="ACHOSM_RS11245"
                     /old_locus_tag="pgaptmp_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HGU4558764.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5420..8116)
                     /gene="mgtA"
                     /locus_tag="ACHOSM_RS11250"
                     /old_locus_tag="pgaptmp_002250"
     CDS             complement(5420..8116)
                     /gene="mgtA"
                     /locus_tag="ACHOSM_RS11250"
                     /old_locus_tag="pgaptmp_002250"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HGU4558765.1"
                     /translation="MFKEIFTRLTRHLPSRLVHRDPLPGVQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCYEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(8257..8310)
                     /gene="mgtL"
                     /locus_tag="ACHOSM_RS11255"
                     /old_locus_tag="pgaptmp_002251"
     CDS             complement(8257..8310)
                     /gene="mgtL"
                     /locus_tag="ACHOSM_RS11255"
                     /old_locus_tag="pgaptmp_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HGU4558766.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8495..9442
                     /gene="treR"
                     /locus_tag="ACHOSM_RS11260"
                     /old_locus_tag="pgaptmp_002252"
     CDS             8495..9442
                     /gene="treR"
                     /locus_tag="ACHOSM_RS11260"
                     /old_locus_tag="pgaptmp_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HGU4558767.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SSQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPANLS"
     gene            9561..10982
                     /gene="treB"
                     /locus_tag="ACHOSM_RS11265"
                     /old_locus_tag="pgaptmp_002253"
     CDS             9561..10982
                     /gene="treB"
                     /locus_tag="ACHOSM_RS11265"
                     /old_locus_tag="pgaptmp_002253"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HGU4558768.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLLTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11032..12687
                     /gene="treC"
                     /locus_tag="ACHOSM_RS11270"
                     /old_locus_tag="pgaptmp_002254"
     CDS             11032..12687
                     /gene="treC"
                     /locus_tag="ACHOSM_RS11270"
                     /old_locus_tag="pgaptmp_002254"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGU4558769.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRISDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGETWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            13081..15219
                     /gene="nrdD"
                     /locus_tag="ACHOSM_RS11275"
                     /old_locus_tag="pgaptmp_002255"
     CDS             13081..15219
                     /gene="nrdD"
                     /locus_tag="ACHOSM_RS11275"
                     /old_locus_tag="pgaptmp_002255"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGU4558770.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15377..15841
                     /gene="nrdG"
                     /locus_tag="ACHOSM_RS11280"
                     /old_locus_tag="pgaptmp_002256"
     CDS             15377..15841
                     /gene="nrdG"
                     /locus_tag="ACHOSM_RS11280"
                     /old_locus_tag="pgaptmp_002256"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HGU4558771.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15886..16272)
                     /gene="cybC"
                     /locus_tag="ACHOSM_RS11285"
                     /old_locus_tag="pgaptmp_002257"
     CDS             complement(15886..16272)
                     /gene="cybC"
                     /locus_tag="ACHOSM_RS11285"
                     /old_locus_tag="pgaptmp_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGU4558772.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16455..17807)
                     /gene="pmbA"
                     /locus_tag="ACHOSM_RS11290"
                     /old_locus_tag="pgaptmp_002258"
     CDS             complement(16455..17807)
                     /gene="pmbA"
                     /locus_tag="ACHOSM_RS11290"
                     /old_locus_tag="pgaptmp_002258"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HGU4558773.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDEPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17901..18452
                     /gene="yjgA"
                     /locus_tag="ACHOSM_RS11295"
                     /old_locus_tag="pgaptmp_002259"
     CDS             17901..18452
                     /gene="yjgA"
                     /locus_tag="ACHOSM_RS11295"
                     /old_locus_tag="pgaptmp_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HGU4558774.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(18608..19981)
                     /gene="mpl"
                     /locus_tag="ACHOSM_RS11300"
                     /old_locus_tag="pgaptmp_002260"
     CDS             complement(18608..19981)
                     /gene="mpl"
                     /locus_tag="ACHOSM_RS11300"
                     /old_locus_tag="pgaptmp_002260"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HGU4558775.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20157..21155
                     /gene="fbp"
                     /locus_tag="ACHOSM_RS11305"
                     /old_locus_tag="pgaptmp_002261"
     CDS             20157..21155
                     /gene="fbp"
                     /locus_tag="ACHOSM_RS11305"
                     /old_locus_tag="pgaptmp_002261"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HGU4558776.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(21188..22185)
                     /gene="yjfF"
                     /locus_tag="ACHOSM_RS11310"
                     /old_locus_tag="pgaptmp_002262"
                     /pseudo
     CDS             complement(21188..22185)
                     /gene="yjfF"
                     /locus_tag="ACHOSM_RS11310"
                     /old_locus_tag="pgaptmp_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
     gene            complement(22172..23194)
                     /gene="ytfT"
                     /locus_tag="ACHOSM_RS11315"
                     /old_locus_tag="pgaptmp_002263"
     CDS             complement(22172..23194)
                     /gene="ytfT"
                     /locus_tag="ACHOSM_RS11315"
                     /old_locus_tag="pgaptmp_002263"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HGU4558777.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGAGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(23208..24710)
                     /gene="ytfR"
                     /locus_tag="ACHOSM_RS11320"
                     /old_locus_tag="pgaptmp_002264"
     CDS             complement(23208..24710)
                     /gene="ytfR"
                     /locus_tag="ACHOSM_RS11320"
                     /old_locus_tag="pgaptmp_002264"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HGU4558778.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKLQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRLQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(24850..25806)
                     /gene="ytfQ"
                     /locus_tag="ACHOSM_RS11325"
                     /old_locus_tag="pgaptmp_002265"
     CDS             complement(24850..25806)
                     /gene="ytfQ"
                     /locus_tag="ACHOSM_RS11325"
                     /old_locus_tag="pgaptmp_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HGU4558779.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26116..26646
                     /gene="ppa"
                     /locus_tag="ACHOSM_RS11330"
                     /old_locus_tag="pgaptmp_002266"
     CDS             26116..26646
                     /gene="ppa"
                     /locus_tag="ACHOSM_RS11330"
                     /old_locus_tag="pgaptmp_002266"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HGU4558780.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27025..27366)
                     /gene="ytfP"
                     /locus_tag="ACHOSM_RS11335"
                     /old_locus_tag="pgaptmp_002267"
     CDS             complement(27025..27366)
                     /gene="ytfP"
                     /locus_tag="ACHOSM_RS11335"
                     /old_locus_tag="pgaptmp_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HGU4558781.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(27369..31157)
                     /gene="tamB"
                     /locus_tag="ACHOSM_RS11340"
                     /old_locus_tag="pgaptmp_002268"
     CDS             complement(27369..31157)
                     /gene="tamB"
                     /locus_tag="ACHOSM_RS11340"
                     /old_locus_tag="pgaptmp_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HGU4558782.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAEVAQEEVVEPKIENPQPEEKPLGETLKDL
                     FSRPVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALD
                     IDSNQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNL
                     SGPVDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTL
                     SMRTAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGE
                     LTLNGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTL
                     KGNSYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGL
                     VKVRGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPD
                     VNINLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPV
                     GPWSLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLA
                     MLKPFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAF
                     QTLNLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMIN
                     PIFTRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFN
                     GMRSTLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDV
                     VFEATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPIN
                     SNLIVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVR
                     KGELLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAA
                     LSYLLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDS
                     SQVVVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF
                     "
     gene            complement(31154..32887)
                     /gene="tamA"
                     /locus_tag="ACHOSM_RS11345"
                     /old_locus_tag="pgaptmp_002269"
     CDS             complement(31154..32887)
                     /gene="tamA"
                     /locus_tag="ACHOSM_RS11345"
                     /old_locus_tag="pgaptmp_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HGU4558783.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGITLGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            33093..33731
                     /gene="msrA"
                     /locus_tag="ACHOSM_RS11350"
                     /old_locus_tag="pgaptmp_002270"
     CDS             33093..33731
                     /gene="msrA"
                     /locus_tag="ACHOSM_RS11350"
                     /old_locus_tag="pgaptmp_002270"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HGU4558784.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PAVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            34054..35409
                     /gene="paeA"
                     /locus_tag="ACHOSM_RS11355"
                     /old_locus_tag="pgaptmp_002271"
     CDS             34054..35409
                     /gene="paeA"
                     /locus_tag="ACHOSM_RS11355"
                     /old_locus_tag="pgaptmp_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGU4558785.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKDKDKEESV
                     A"
     gene            complement(35471..35677)
                     /gene="ytfK"
                     /locus_tag="ACHOSM_RS11360"
                     /old_locus_tag="pgaptmp_002272"
     CDS             complement(35471..35677)
                     /gene="ytfK"
                     /locus_tag="ACHOSM_RS11360"
                     /old_locus_tag="pgaptmp_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HGU4558786.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            36002..36556
                     /gene="ytfJ"
                     /locus_tag="ACHOSM_RS11365"
                     /old_locus_tag="pgaptmp_002273"
     CDS             36002..36556
                     /gene="ytfJ"
                     /locus_tag="ACHOSM_RS11365"
                     /old_locus_tag="pgaptmp_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGU4558787.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNVTLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(36646..>37380)
                     /gene="ytfI"
                     /locus_tag="ACHOSM_RS11370"
                     /old_locus_tag="pgaptmp_002274"
     CDS             complement(36646..>37380)
                     /gene="ytfI"
                     /locus_tag="ACHOSM_RS11370"
                     /old_locus_tag="pgaptmp_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HGU4558788.1"
                     /translation="ATQIDKDSYQVLSSKNETVKAMDKFLISFSLKDNGAEYTMTLRG
                     SGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQA
                     IGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFSIVIESLGF
                     ALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAVDSD
                     ITEEEICEKYKSTIEAFKKEQLALPV"
BASE COUNT         9066 a        10029 c         9257 g         9028 t
ORIGIN      
        1 acttcattaa ctcactgcca tcattatttt tgatataaga tgaggactca atatgagcca
       61 tcatatttct tcccgcttcg ttggagcgtt tgctaaaatc cacataaccc agggtaaata
      121 tttttaccaa aaactccaaa aatgctctgg gagaaagaat attgcttctg ctattctgcc
      181 ctccggcaat tcgggcaaaa tcattcattc ccacatacca ggtatctaag tcttcatttt
      241 tttgtttaca attgtcggct gatgtaatgt tttgagaaaa attccaacct gatattttgc
      301 tcatgacgga ctccttgagt taataagaac gtctaagcat tacaaatttt actcacaaaa
      361 aaatatcaaa aaacgctctt ttcacaaact cgccgtaaac ctggagttat ttacggcgaa
      421 aagtcatcac gccatcagca tcttcactac tgctttgcga acctgggctg gtgcacccac
      481 tgcgcacagc ggtttatgca cttcccccgg ataaaacaca acaaaatcac cttcattaag
      541 gataactgtt ttctcatcaa cgccttccgg caaaaatgcg atgtctttat cagctaacca
      601 gtcggtatcc ggcgtgcctg caggttgcgt gctgaaggtc atgccttcct gaccttttaa
      661 cacaatctga atgtcgagat agcgggcatg gtactccgca cggcgggctt cgtacggctc
      721 ggtcatatct tccgagataa gataaaacag acgattgcct tcgatatcgt gcttgccctt
      781 tggcgtttct gccgtaacgt gtgctttgat atgctcaatc gcctggcgta actcctgggg
      841 tagccacggc tgaagattat gaatatttcc gatgatcatc ttttccattc cttaattaaa
      901 actacgtttc atttctatcg ttatacgaaa agatgatcaa ttccgctagc cttaacgcgt
      961 gaattattgt cttatcacaa cttatcggca ggcgcgttcc cgcctttcgg atcaaggcca
     1021 gcatcactcc ttgagaagag tttttatcgc ttcccccgcc agggcagctg atgcacagag
     1081 ttgttcgatg gagtgcccct cccgtgcctt cgcagaaagc cctgacatca cggtactcat
     1141 aaaatcagtc acacattgtg cactttgtgg atgccgcctg gcgatatagt cataaatggc
     1201 cgtttctgcg gcgtgataat attgaacggc aatatcacgc gcttgtggat catgactatg
     1261 aatgccttca agaaccatac agccagcgca gccaccgttt tggctatatc tgcgcgccgc
     1321 ttcttttaat acctcagcca ggcactcccc taccggacga tcatcacgaa gaatatcggc
     1381 aagcggaata gcttccgtac cgacatattc attgagtaca cggctaaata acccagcctt
     1441 attgccaaaa gccgcgtaga ggctcggggg gttaatacca agataatcag taagttcagc
     1501 aacactgaca gcatcgaaac ctttttggtg aaaaagcact tttgccgcag agactgcctg
     1561 ctcaggagcg aaacgtctgg gacgacctgg aacgcgagat tgttttttag tgatcattac
     1621 aaaacttgtt gacagaaagt taaaacagtt ttgtaatgca tgttacataa taaatcaagg
     1681 agtccttatg ggcgctttta caggtaagac agttctcatc ctcggtggta gccgtggaat
     1741 cggtgccgct atcgtacgtc gtttcgtcac cgatggggcc aatgtacgat tcacctatgc
     1801 ggggtcgaaa gatgccgctg aacgcctggc acaagagacg ggagcgacag cagtattcac
     1861 agatagtgct gacagagacg ctgtcattga tgtcgttcgt aagagcggcg cattggatat
     1921 cctggtggta aatgcaggta ttggcgtctt tggcgatgcc ctggaattaa atgccaacga
     1981 tattgatcgc cttttcaaaa tcaatattca tgctccttac catgcctctg ttgaagccgc
     2041 ccggcagatg cccgaaggcg ggcgcatctt aatcatcggc tccgtgaatg gcgatcgtat
     2101 gcctgttgca ggcatggctg cttatgccgc cagtaaatct gccctacaag gcatggcgcg
     2161 cgggctggcc cgtgattttg gaccgcgtgg gatcaccatc aacgtcgtcc agccagggcc
     2221 aattgatacc gacgctaatc ccgccaacgg gccaatgcgc gatatgttgc atggttttat
     2281 ggccatcaaa agacatgggc aaccggaaga ggtcgctggt atggtcgcat ggttagcagg
     2341 gccagaagcc agttttgtta ccggcgcgat gcataccatt gatggcgcgt ttggcgcata
     2401 accgactacg ctcaattaag cccagccatt tcccatgatg tctgggtttt gtttactcac
     2461 gtcgtccgct aaaagcggct cccggtaaat ataaatcttc tgttcatgtc gtaaatcttg
     2521 aggataacca aatggtagaa agaaccgctg ttttccctgc tggccgacac tcactgtatg
     2581 cagaacatcg ttattctgcg gctattcgtt ccggcgattt gctgtttgtt tccgggcaag
     2641 taggaagtcg agaggacgga tcaccagaac ccgattttca gcaacaagtc agactggcat
     2701 ttgataattt gcatgcgacc ctggcagctg cgggatgcac ttttgacgat atcattgatg
     2761 ttacgagctt tcataccgat ccagaaaaac aatttgaaga catcatgacg gtgaaaaatg
     2821 aaatatttag cgcgccacct tatccaacct ggacggcggt gggcgttaca tggctggcag
     2881 gctttgattt tgaaattaaa gtgatagcgc gcatccctga gctataagca atagtgttag
     2941 ccgttcgttt tcacactccg ccctataagt cggatgaatg gaataaaatg catatctgat
     3001 tgcgtgaaag tgaaaaagaa aaaagcagga aatgtctgca attattgata ccgaacgaca
     3061 gttcccctcc agaatcacag taaataaaaa tgcatatatt ttgactttag attcaaataa
     3121 accgtttgcg ctgacaaaat attgcatcaa atgcttgcac cgcttctgac gatgagtata
     3181 atgccggaca atttgccggg aggatgtatg gttcagtgtg ttcgacattt tgtcttaccg
     3241 cgtctgaaaa aagacgctgg cctgccgttt ttcttcccgt tgatcaccca ttcccagccc
     3301 ctcaatcgag gggctttttt ttgcccaggc gtcaggagat aaaagatggc taatccgcta
     3361 tatcagaaac atatcatttc cataaacgac cttagtcgcg atgaccttaa tctggtgctg
     3421 gcgacagcgg cgaaactgaa agcaaacccg caaccagagc tgttgaagca caaagtcatt
     3481 gccagctgtt tcttcgaagc ctctacccgt acccgcctct ctttcgaaac ttccatgcac
     3541 cgcttgggtg ccagcgtggt gggcttttcc gacagcgcca atacatcact gggtaaaaag
     3601 ggcgaaacgc tggccgatac catttcggtt atcagcactt acgttgatgc aatagtgatg
     3661 cgtcatccgc aggaaggtgc ggcgcgcctg gccaccgagt tttccggcaa tgtaccggta
     3721 ctgaatgccg gtgatggctc caaccaacat ccgacgcaaa ccttgctgga cttattcacc
     3781 attcaggaaa cccaggggcg tctggacaat ctccacgtcg caatggttgg tgacctgaaa
     3841 tatggccgca ccgttcactc cctgactcag gcgttagcga agttcgacgg caaccgtttt
     3901 tacttcattg cgccggacgc gctggcaatg ccgcaataca ttctggatat gcttgatgaa
     3961 aaagggatcg catggagtct gcacagctct attgaagaag tgatggcgga agtagacatc
     4021 ctgtatatga cccgcgtgca aaaagagcgc ctggacccat ccgaatacgc caacgtgaaa
     4081 gcgcagtttg ttcttcgcgc cagcgatctc cacaacgcca aagccaatat gaaagtgctg
     4141 catccgctgc cgcgtgttga tgagattgcc accgatgtgg ataaaacgcc acacgcctgg
     4201 tacttccagc aggcaggcaa tgggattttc gctcgccagg cgttactggc actggttctg
     4261 aatcgcgatc tggtactgta aggggaaata gagatgacac acgataataa attgcaggtt
     4321 gaagctatta aacgcggcac ggtaattgac catatccccg cccagatcgg ttttaagctg
     4381 ttgagtctgt tcaagctgac cgaaacagat cagcgcatca ccattggtct gaacctgcct
     4441 tctggcgaga tgggccgcaa agatctgatc aaaatcgaaa ataccttttt gagtgaagac
     4501 caggtagatc aactggcatt gtatgcgccg caagccacgg ttaaccgtat cgacaactat
     4561 gaagtggtgg gtaaatcgcg ccccagtctg ccagagcgca tcgacaatgt gctggtctgc
     4621 ccgaacagca actgtatcag ccatgccgaa ccggtttctt ccagctttgc cgtgcgaaaa
     4681 cgcgccaatg atatcgcgct caaatgcaaa tactgtgaaa aagagttttc ccataatgtg
     4741 gtgctggcca attaattgcg gttggtaata aaagtctggc tccctataat gagccagact
     4801 ttttaccgct gtaataaagg agaaatcatg agcaaaacta tcgcgacgga aaatgcaccg
     4861 gcagctatcg gtccttacgt acagggcgtt gatctgggca atatgatcat cacctccggt
     4921 cagatcccgg taaatccgaa aacgggcgaa gtaccggcag acgtcgctgc acaggcacgt
     4981 cagtcgctgg ataacgtaaa agcgatcgtc gaagccgctg gtctgaaagt gggcgacatc
     5041 gttaaaacta ccgtgtttgt aaaagatctg aacgacttcg caaccgtaaa cgccacttac
     5101 gaagccttct tcaccgaaca caacgccacc ttcccggcac gttcttgcgt tgaagttgcc
     5161 cgtctgccga aagacgtgaa gattgagatc gaagcgatcg ctgttcgtcg ctaatcttga
     5221 tggaaatccg ggctatcatg cccggattaa gtctgatgac aaacgcaaaa tcgcctgatg
     5281 cgctacgctt atcaggccta cgtgattcct gcaatttatt gaatttgttg gccggataag
     5341 gcatttacgc cgcatccggc atgaacaaag cttactttgt ctgcaatctg aatcggggct
     5401 atcgtgccca gtttattctt tattgccagc cgtaacgacg gctatagaac cctttcacca
     5461 actgggttaa tgtcatatac cctgccagaa tcgcaaccag ccacgggaaa tagcttaacg
     5521 gcagcgcctg taattgcaga taactggcca gcggtgaaaa cggcaatgcg atcccgacaa
     5581 tcatcacgat cacggtcatg atcattaacg gccacgatgc acagctctga ataaacggca
     5641 cacggcgggt gcggatcata tgcacaatca gcgtttgcga cagtaagccc accacaaacc
     5701 atcccgactg gaacagcgtt tgcgtttccg gcgtgttggc atggaatacc caccacatca
     5761 ggcaaaacgt cagaatatcg aagatcgagc tgatcggtcc gaagaagatc ataaagcgcc
     5821 ccagatccgc cggattccaa cgctgcggct tttgaatttg ctcgtcgtcg acgttatcaa
     5881 acgggatcgc cacctgtgac acatcgtaca gcaggttctg aataagcaag tgtaacggca
     5941 acatcggcag gaagggcaag aaagcactcg ccaccagcac gctgaacaca ttaccgaagt
     6001 tagaactcgc cgtcattttg atgtatttca gcatgttggc gaaagtgcga cgtccctcaa
     6061 taaccccctc ttccagcacc atcaggcttt tttccagcag gatgatatca gccgcttcac
     6121 gggcaatatc taccgcgccg tccacagaaa tgccgatatc cgcagcgcgt aaggcgggcg
     6181 catcattaat accatcgccc ataaagccaa ccacatgccc ttcgcgcttc agtaaggtca
     6241 caatgcgttc tttatgcatc ggcgtcaggc gggcaaacag cgtggtgcgc tgtgcaagat
     6301 ttgccagttc gtcgtcagat agcgtttcaa tatcactacc aatgaccacc tctcccgcgt
     6361 ccagccccac ttcatagcac actttcgctg caactaactc actgtcgcca gtaaggattt
     6421 ttacggtaat tccactcgct tttaatgcct tcagtgccgg agcggttgtc tcttttggcg
     6481 gatcaaggaa agcaatatat ccttcgagga tcaggtcgga ttcatccgcc cgctggtaat
     6541 ctccttcacg cgctggcagg tatttcgtcg ccaccgcaac cacgcgcagc ccctgacgat
     6601 tcagcgtatc agtaacccgc ttaatcttac gcagcatgat gtcatcgagc ggcacaatct
     6661 cgccattgtg acgcacctgc gaacacacat tgaggatttc ctgcaatgca cctttgcaaa
     6721 ccagctgatg gtgctcagta ttttctgcca ctaccaccga catccggcga cgctcgaaat
     6781 caaacggaat ctcatcgatt ttttgccaac gactggccag cgagcgcgct gactcttcat
     6841 ccgtaccttc gagcaccgct gtatcgagca ggtttttaag tccggtctga taatgactgt
     6901 tcaaccacgc gctatgcagc acgcgttcgc tggttttacc ggaaatatcg gtatgattct
     6961 ccagcacaat tttatcctgt gtcagggtgc cggttttatc agtgcacaga atatccattg
     7021 cgccaaagtt ctgaatagca tccagatgtt tgacgatcac tttctgtttc gacagcttta
     7081 ctgcgccgcg cgccagcgtc gaggtaacaa tcatcggcaa catttccggc gttaggccta
     7141 ccgcgaccga aagcgcaaac agcgccgctt cccaccagtc gcctttggtg taaccattga
     7201 ttaacagcac caccggtgcc atcaccagca taaagcgaat cagcagcata ctgacgcggc
     7261 tgatcccttg ctgaaaggca ttcggctcgc tttcctgctc actaacacgc cccgccagtt
     7321 gaccaaacca ggtattggca cctgtagcaa tcaccattgc ttgtgccgtg ccgctcacca
     7381 cggtggtgcc cataaaacac agcgtgtcgc actccagcgg attgctgtgc tccggctggc
     7441 gagtggtagc tgctttttcc acgggcagag actcaccggt taacgacgcc tgagcgacga
     7501 acagatcccg cgcctgcaag atacgtaaat ctgccgggat catatctccc gccgccagtt
     7561 ttataatatc gccgggcacc agctggtcga tcgggatctc cagccagcca ttttcgcctt
     7621 tgtcgttaat tacgcgcagc accgtcgcgg tattgctgac catcgctttc agggcatctg
     7681 ccgctttagt ggaacgtgct tcctgaataa agttcagcaa cgttgaaata gcgaccatta
     7741 gcgcgataac gcccgcggca aataaatctt ccgtggcgta agaaatagcg ccgagaatgg
     7801 tgagtaaaat attaaagggg ttgcgatagc agacccataa atgtacccac cacggcgacg
     7861 gctgttgtgc gggtaattta ttttcaccat gttgttcgcg ggcagattcc acttccgcct
     7921 gatttaaccc ttccggatgg gtgtcgaacg ttttccacaa ttcttcttcg ggcatcaccg
     7981 ccattttcag gcaatgcgca cttaaggacg gcgggaccac cgtattcact gtctgctgca
     8041 cgccaggcaa tggatcacga tgaaccagac gggaaggtaa atggcgagtg agccgggtaa
     8101 aaatttcttt aaacataagg agtccctccg cactgtctga aacagtacag aaatgtcttc
     8161 aggcgtggcg aagccttacc tgaaggtaat aaagctgagc agggacgtta aaacgtcacg
     8221 gccttacgca tccggtaacg ccgtgaaaag acgggcttac cggaaaagtt ttaatctccg
     8281 tcgagggaga ggcgtgggat caggttccat ataacctccg gtaagtgaaa aaagcgccgc
     8341 aaataataag gagaatccaa taatattcgt agcatctatg gcgatataat acctgctggc
     8401 aactaaacca acgataaacc agactttacc cattgctgaa tgcacgggta aagttaggct
     8461 caaataatta aacaacacgt tacaggacaa caggatgcaa aatcggctga ccatcaaaga
     8521 catcgcgcgc ttaagcggcg tggggaaatc tacagtttcc cgggtgctga ataacgaaag
     8581 cggcgtgagc cagcgcaccc gcgagcgtgt tgaagcagtg atgaatcagc atggattttc
     8641 cccttcccgc tctgcgcgcg ctatgcgtgg gcaaagcgat aaagtggtcg ccatcattgt
     8701 tacccgtctg gattcgttgt cagaaaatct cgccgttcaa accatgctac cagcgttcta
     8761 tgaacaaggt tacgacccaa tcatgatgga aagtcagttt tcctcacaat tagttgccga
     8821 acatttgggg gtgctgaaac ggcgtaatat cgacggcgta gtgttgttcg gttttactgg
     8881 cataacagaa gaaatgttag cccactggca gtcatcgctg gttctgctgg cgcgtgacgc
     8941 aaaaggcttt gcttcggtct gttatgacga cgaaggggca atcaagatcc tgatgcaacg
     9001 gctgtatgac caggggcatc gtaatatcag ttatctcggc gtgccgcaca gtgacgtgac
     9061 aaccggtaag cgacgtcacg aagcctacct ggcgttctgc aaagcgcata aactgcatcc
     9121 cgttgccgcc ctgccagggc ttgctatgaa gcaaggctat gagaacgtag caaaagtgat
     9181 tacgcctgaa actaccgcct tactgtgcgc aaccgacacg ctggcacttg gcgcaagtaa
     9241 atacctgcaa gagcaacgca tcgacacctt gcaactggcg agcgtcggta atacgccgtt
     9301 aatgaaattc cttcatccgg agatcgtcac cgtagatcct ggttacgccg aagctggacg
     9361 ccaggcggct tgccagttga tcgcgcaggt aaccggacgc agcgaaccgc aacaaatcat
     9421 catccccgcc aacctgtcct gatcgtttcc tgaacgataa attgtgatct tcgctgcgtt
     9481 tcgggaacgt tcccgttttt aaatttttcc gcgcaatata ttctgcagcc aaccaaaaat
     9541 gtcatctgcc atggggcttt atgatgagca aaataaacca aacggatatc gatcggttga
     9601 ttgaactggt cggcgggcgc ggcaatattg cgacggtgag ccactgtatt actcgcctgc
     9661 gctttgtcct caaccaaccg gccaatgcca gaccgaaaga aattgagcaa ctccccatgg
     9721 tgaaaggctg tttcaccaat gccgggcaat ttcaggtggt gattggcacc aacgtgggtg
     9781 attactatca agcactgata gcgtcaaccg gacaggcgca ggttgataaa gagcaggtaa
     9841 aaaaagccgc ccggcagaat atgaaatggc atgagcagtt gatctctcat ttcgcggaga
     9901 tcttcttccc gttgctgccc gcgttgatca gcggcggttt gatcctcggt tttcgcaatg
     9961 tgatcggcga tttgcccatg agcaacggtc aaacgctggc gcaaatgtac ccttccctgc
    10021 aaacgatcta cgattttctg tggttgatcg gtgaagcgat cttcttctac ctgccggtcg
    10081 ggatttgctg gtcagcggtg aaaaaaatgg gcggtacacc gatcctcggt atcgtgcttg
    10141 gcgtaacgct ggtttctcca cagctgatga atgcttatct gctcgggcag cagctgccgg
    10201 aagtgtggga ctttggcatg ttcagcatcg ccaaagtggg ctatcaggca caggtgatcc
    10261 cggcactgtt agccgggttg gcactgggcg ttattgaaac tcgccttaaa cgcatcgtgc
    10321 cagattacct ctatctggtg gtggtgcccg tctgttcgct gatcctcgcg gtgttcctcg
    10381 cccatgcgct gattggtcca tttggtcgca tgattggcga tggcgttgcc tttgcggtac
    10441 gtcacctgct gaccggcagc tttgctccga ttggcgcagc attgtttggc ttcctgtacg
    10501 cgccgctggt gatcaccggc gtacaccaga ccacgcttgc tattgatttg cagatgattc
    10561 aaagcatggg tggcacgcca gtgtggccgc tgattgcgct gtcgaatatc gctcagggct
    10621 ccgccgtaat aggcattatc atttccagcc gcaagcacaa tgagcgcgag atctccgtgc
    10681 ctgccgctat ctccgcctgg cttggggtca ctgagcctgc aatgtacggc atcaacctga
    10741 aatatcgctt cccgatgctg tgcgcgatga ttggttctgg tctggcagga ttgctatgcg
    10801 gcctgaacgg cgttatggcg aatggtatcg gcgtaggcgg cctgccggga attctctcga
    10861 ttcaaccgag ctactggcag gtgtttgcgc tggcaatggc tatcgccatc atcatcccga
    10921 ttgtactcac ctcgtttatc taccagcgga aataccgcct gggcacgctg gacattgttt
    10981 aattttcttc ggggcgcaat tgcgccccct cgcatttgca ggaataacgt aatgactaat
    11041 cttccccact ggtggcaaaa cggcgttatc taccagattt atccaaagag ttttcaggac
    11101 accacgggta gcggcaccgg cgatttacgt ggcgttatcc aacgcctgga ctatctgcat
    11161 aaactgggcg ttgatgccat ctggctaacc cccttttatg tctctcccca ggtcgataac
    11221 ggttacgatg tagcgaacta tacggcgatt gatcccacct acggcacgct ggacgatttt
    11281 gacgaactgg tgacgcaggc aaaatcgcgc gggattcgta tcattctcga tatggtgttt
    11341 aaccatacct ctacccaaca tgcctggttt cgcgaggcgc tgaacaaaga aagcccttac
    11401 cgccagtttt atatctggcg cgatggcgaa ccagaaacgc caccgaacaa ctggcgttca
    11461 aaatttggcg gtagtgcgtg gcgctggcat gcggaaagcg aacagtacta tttgcatctc
    11521 tttgcaccag aacaggcgga tctcaactgg gagaatccag cggtacgcgc agagctgaaa
    11581 aaagtctgtg aattctgggc cgatcgtggg gtcgacgggt tgcgcctgga tgtggtgaat
    11641 ctgatctcca aagacccgcg tttccctgaa gacctggatg gcgacgggcg tcgcttctac
    11701 accgacgggc cacgagcaca cgagtttttg cacgagatga accgcgatgt gtttacgcca
    11761 cgcgggttaa tgaccgtagg tgaaatgtcc tccaccagcc ttgagcattg ccagcgatac
    11821 gcggcactga caggcagtga attgtcgatg acctttaatt ttcatcacct gaaggtcgat
    11881 tatcccggtg gtgaaaaatg gacgctggct aaacctgact ttgtggcgtt gaaaacattg
    11941 ttccgccact ggcaacaagg aatgcacaac gtggcatgga atgccttgtt ctggtgtaac
    12001 cacgatcagc cgcgcattgt ttctcgcttt ggtgatgaag gtgaataccg cgtgcctgcg
    12061 gcaaaaatgc tggcgatggt gctgcatggc atgcagggaa cgccgtatat ctaccagggc
    12121 gaagagattg gcatgaccaa cccgcatttc acgcgcattt ctgactatcg cgacgtagag
    12181 agcctcaata tgtttgccga gctgcgcaac gatgggcgtg atgccgacga gttattggca
    12241 atcctcgcca gtaaatcccg tgacaacagc cgcacgccca tgcaatggag caacggcgat
    12301 aatgccgggt ttacggctgg cgaaacgtgg attggcctgg gcgataacta tcaacaaatc
    12361 aacgtagaag ccgcgctggc cgatgactcc tcggtgtttt acacctacca aaagttaatc
    12421 gcactgcgta agcaagaagc catcctgaca tggggcaatt accaggatct gctgccaaac
    12481 agccctgtat tgtggtgcta tcgccgtgaa tggaaggggc aaaccttgct ggtcattgcc
    12541 aaccttagcc gtgagatcca accctggcag ccagggcaaa tgcgcggcaa ctggcaactt
    12601 gtgatgcata actacgaaga agcctcacca caaccctgtg ccatgacttt acggcctttt
    12661 gaggctgtct ggtggttaca gaagtaaatc ttccctaagc cccggtaatg ccggtcattc
    12721 cggggttttg tctgtcagta tgttgttttt gttgattttt caaccagaaa attcattaaa
    12781 aaatttacat atcgctgtag cgcccgtcat ccgtacgctc tgctttttac tttgagctac
    12841 atcaaaaaaa gttaaaacat ccttgatgca aagcactata tatagacttt aaaatgcgcc
    12901 ccaacccaat atgttgtatt aatcgactat aattgctact acagctcccc acgaaaaagg
    12961 tgcggcgttg tggataagcg gatggcgatt gcggaaagca ccggaaaacg aaacgaaaaa
    13021 accggaaaac gcctttccca atttctgtgg ataacctgtt cttaaaaata tggagcgatc
    13081 atgacaccgc atgtgatgaa acgagacggc tgcaaagtgc cgtttaaatc agagcgcatc
    13141 aaagaagcga ttctgcgtgc agctaaagca gcggaagtcg atgatgccga ttattgcgcc
    13201 actgttgccg cggttgtcag cgagcagatg cagggccgca accaggtgga tatcaacgag
    13261 atccagaccg cagttgaaaa ccagctgatg tcgggtccat acaaacaact ggctcgtgct
    13321 tacatcgagt accgtcacga tcgcgacatt gaacgtgaaa aacgcggtcg cctgaaccag
    13381 gagatccgtg gtctggtcga gcagaccaac gcctcgttac tcaacgaaaa cgccaacaaa
    13441 gacagcaagg tgattccaac ccagcgcgac ctgctggccg ggatcgtggc taaacactat
    13501 gcacgccagc acctgctgcc gcgtgacgtg gtgcaggcac atgagcgtgg cgatattcac
    13561 tatcacgatc tcgattactc gccgttcttc ccgatgttca actgcatgtt gatcgacctg
    13621 aaaggcatgc tgacccaggg ctttaaaatg gggaacgccg agattgaacc gccgaagtcg
    13681 atctctacgg caaccgcggt aactgcgcag attattgctc aggtcgccag ccatatttat
    13741 ggcggcacca ccattaaccg tatcgatgaa gtgctggcgc cgtttgttac cgccagctac
    13801 aacaaacatc gcaagaccgc agaagagtgg aacatcccgg acgccgaagg ctacgctaac
    13861 tctcgtacca tcaaagagtg ctacgatgcc ttccagtcgc tggagtacga agtaaacacc
    13921 ctgcacaccg ccaacggtca gacgccgttt gtaacctttg gttttggtct gggcaccagc
    13981 tgggaatcgc gcctgattca ggaatcgatc ctgcgtaacc gtatcgcagg cctcggtaaa
    14041 aaccgtaaaa ctgcggtgtt cccgaaactg gtgtttgcga ttcgcgatgg cctgaaccat
    14101 aaaaaaggcg atccgaacta cgacatcaaa cagctggcgc tggagtgcgc aagcaagcgc
    14161 atgtatccgg atatcctgaa ctacgatcag gtagtgaaag tcaccggttc gtttaaaacc
    14221 ccgatgggct gccgcagctt cctcggcgtg tgggaaaatg aaaacggcga gcagatccac
    14281 gatggtcgta acaacctcgg cgtgatcagc ctgaacctgc cgcgtattgc tctggaagca
    14341 aaaggcgatg aagccacctt ctggaagctg ctggatgaac gtctggtgct ggcacgtaag
    14401 gcactgatga cccgtatcgc tcgtctcgaa ggcgtgaaag cgcgcgtcgc cccgatcctc
    14461 tatatggaag gtgcttgtgg cgtacgtctg aatgctgacg atgatgtttc tgaaatcttc
    14521 aaaaacggtc gtgcgtctat ttcgctgggt tacatcggca tccacgaaac cattaacgcg
    14581 ctgttcggcg gcgagcatgt ttacgacaac gagcagcttc gcgcgaaagg tatcgccatt
    14641 gttgaacgtc tgcgtcaggc agtggatcag tggaaagaag aaacgggtta tggtttcagt
    14701 ctctacagca cgccgagtga aaacctgtgc gaccgcttct gccgtctcga tactgctgag
    14761 tttggcgtgg tgccgggcgt gaccgataaa ggttactaca ccaacagttt ccaccttgat
    14821 gtggagaaga aggtgaaccc gtacgacaag atcgactttg aagcgcctta cccgccgctg
    14881 gcgaacggtg gtttcatttg ctacggcgag tatccaaaca ttcagcacaa cctgaaagcg
    14941 ctggaagatg tctgggatta cagctatcag catgtaccgt attacggcac caatacaccg
    15001 attgatgagt gctacgagtg tggctttacc ggtgagttcg agtgcaccag caaaggcttc
    15061 acttgcccga aatgtggtaa ccatgacgcc tcccgtgtgt cggtaactcg ccgcgtgtgc
    15121 ggatatttag gtagcccgga tgcacgtcca tttaacgccg gtaagcagga agaagttaag
    15181 cgccgcgtta aacatttggg aaatgggcaa ataggttaat cttcactcta cgttaagcaa
    15241 atgccggatg cggcgtaaac gccttatccg gcctacattt ggcaactgct ccaggcctga
    15301 taagacgcga cagcgtcgca tcaggcgttg attaccggat gcatcctata cgcggactga
    15361 taagccaggc gaaacaatga attatcatca gtactatcct gtcgacatcg tcaacggccc
    15421 cggcactcgc tgcaccctgt ttgtctccgg gtgtgttcat gaatgccccg gttgctataa
    15481 caaaagcaca tggcgggtaa attccggtca gacatttact aaagcaatgg aagaccagat
    15541 tattaacgat ctgaatgaca ctcgtatcaa acgccagggt atttccctct ccggcggcga
    15601 tccgctgcat ccgcaaaacg tgccggatat tctgaaactg gtaaaacgca tccgcgccga
    15661 gtgtccgggt aaagacatct gggtgtggac aggctataaa ctcgacgaac tcaacgctgc
    15721 gcaaatgcag gttgttgatt tgattaacgt actggtcgac ggcaaatttg tgcaggattt
    15781 aaaagaccct tccctgatct ggcgcggcag cagcaatcag gtggtgcatc atttgcgatg
    15841 ataagttccc tgttacctga gtgcaggcaa cagggaaatg aggaattaac gatacttctg
    15901 gtgataggcg ttgcgggtcg ttttcagttg ctctgcggca gcctgcgctt cttttacttt
    15961 accttcattt gccagcttca gcgcgtcgtc aatctgaccg accagaatgt cgaaaccgtg
    16021 gcggaaatct ttcatttccg ggctgtccgg tgatttatct tcgagcttcg gcggcgttgc
    16081 tttttgcgca tccagcgctg cggcgcgcat cttcgttaac gcgtctttga cttgcgccgc
    16141 gttatccgct ttttcgacca cttttaaatt gtcgttgagg gtttccatat tgtcttcaag
    16201 atcagcggca aacgacgcag aactgaatac caacgaggag actgcaagaa tagctaacag
    16261 gcttttacgc attgctcact tcctttttta tttacacttc atccttcaca ctgcctcatc
    16321 gacggctacg ctcattcgcc cgggtcacgt actgcgagta cgctccccgg ccctcattca
    16381 ctttccgtct cgatgcagtg cgaatgattt tgtgtaattt tttagtttat agcgcggcag
    16441 gtcgcgccag ttttttactg cccggcgatt ttcatctccg gcaacagcac agaaccacac
    16501 tgtatattac tgcgtgtttc aatatcgtta ccgacggtga caatattgcg ccacatatct
    16561 tttaaattac ctgcgatggt gatttcacta acgggatact gaatttcgcc attctctacc
    16621 cagaagcccg ctgcaccacg ggaataatca ccggtaatgg cactcacgcc ctggcccatc
    16681 agctccgtca ccaccagccc ggtgcccatc tctttgagca tttgttcgaa gcttagccct
    16741 tgtccggcaa tccgccagtt gtgaataccg cccgcatgtc cggtgctttt cagccccagt
    16801 ttccgcgccg agtagctggt cagcagccac tgagtcagga tgccatcttt aataatatcg
    16861 cgacgctcgg tgcgcacacc ttcgctgtcg aatggcgtcg acgccagccc tttcagcaga
    16921 tgcggatgct cttcaatggt cagccagtcc ggcagaattt gtttacccag cgaatccagc
    16981 agaaaggtag atttacggta aaccgagcca cccgctatcg cccccaccag atggccaaaa
    17041 agcccggttg ccacttcatt ggcaaaaatg actggcgctt tcatggtgga gagtttacgc
    17101 ggtgacagac gcgataaagt acggcgagcg caatcggccc cgacccactc tggcgtctgc
    17161 aaatcgctca tcgcacgacc aatggtgtag gcgtaatcac gctccatatc gccattttct
    17221 tcggcaatta cacagctgga gagcgaatga cgcgttgagc agtaaccctg caacatgccg
    17281 tggctgttgc caaaaacttt gacaccgtag tggctgttaa agctgccacc ttcggtattg
    17341 gtgatgcgtt tgtccgcctg caatgccgcc tgttctgcgc gggccgccag ttcaatggct
    17401 tcatccgggg aaacttccgc agggtggaac aagtcgagat ctggttcgtc aaaggccagc
    17461 agctctttgt ctgccacgcc ggcacaggga tctggcgagg tataacgggc aatatccagc
    17521 gccgcctgta cagtacgggc aatggcctgc gggcttaaat cggtggatga tgcgctacct
    17581 ttgcggttct ggtgataaac agtgatcccc agcgcgccat cgctattgaa ttcaacattc
    17641 tccacttcac cataacgcgt gcttacgcta atgccggtgg tcttgctgac ggcaacttcc
    17701 gcaccgtccg atttgcctga ggccaactcc agcgcagtgg aaactgcttc ttccagaatc
    17761 ttgcgctgcg cttcaacttg agagattact ttcattgcaa gtgccataat gtagagagtt
    17821 tctctgaagt ctaacagaga accgtttttc agtgcgcatc ttaactggta acattagcct
    17881 ctttttttaa ggagcctgag atgactaagc agcccgaaga ctggctcgac gacgttcccg
    17941 gtgatgacat cgaagacgaa gacgatgaaa ttatctgggt cagtaaaagt gaaattaaac
    18001 gtgatgccga ggagctaaaa cgccttggcg cggaaattgt tgatctgggg aaaaacgcgc
    18061 tggataagat cccgttagat gcggatctgc gcgcggctat tgagctcgcc cagcgtatta
    18121 agatggaagg tcgccgccgc cagttgcagc tcatcggtaa gatgctgcgc cagcgcgacg
    18181 tagagcctat tcgtcaggcg ctggataagc tgaaaaaccg tcacaaccag caggtggtgc
    18241 tgttccataa actggaaaac ctgcgcgatc gtctgatcga tcaaggtgat gacgccatcg
    18301 ctgaggtatt aaatctgtgg cccgatgccg atcgtcagca actgcgtact ctgatccgca
    18361 acgcgaagaa agagaaagaa gggaataagc cgccgaaatc cgcacgccag attttccagt
    18421 atctgcgcga actggcggag aatgaagggt aattttcgtt gttgatgtga gtatggtttg
    18481 ccggatgcgg tgcacctgta agcctgataa gacgcatcaa gcgtcgcatc aggcactatg
    18541 ctcaaatgcc ggatgcggcg tgaacgcctt atccggccta cgcacactat ctcaggctga
    18601 ggccgaatta ctgcgcagct tccgccttct tcgccagacc atccagcagt ttctgatgga
    18661 tcccaccaaa accgccgttg ctcatcacca gaatatggtc gccaggctga gcggttttca
    18721 ccaccatatc tgccagcgta tccacatcgc cactccagtg tgcaggctga acgcaggctt
    18781 ctgccacttc tgccacctgc cacggaatat gcgccggttg cagcaggaag acttcatcgg
    18841 cacgacctaa tgaaggtgcc aaatcgtctt tgcagatccc cattttcatg gtattcgagc
    18901 gcggctccag cacagcaata atgcgcgccg taccaccaac tttgccacgc agcgccgcca
    18961 gcgttgccag aatcgccgtc gggtgatggg caaaatcgtc atataccgtg acgccattcg
    19021 cttcaccacg caactccaga cggcgacgag cattaataaa cgaacccagc gcgttagcgg
    19081 catctgccgg cgctacacca acatggcgag ccgctgcaat cgccatcagg ccattgtgca
    19141 tattatgttc gcctaccagc gaccatttca cttcgcccac tttttcgcca tccagcaaaa
    19201 cttcccattc ggaagcatcg gtggtcagct ttttcgcctg ccagtgaccc tgctcaccca
    19261 ccagctcctg ctcgctccag cagcccatcg ccatggtctg tttcaggttg atgtcatttt
    19321 ccggccagat aatacggccc tgccccggaa cgatacgcac cagatggtgg aactgtttct
    19381 ggatcgcttt caggtcgtca aagatatcgg cgtgatcgaa ctcaaggttg ttgaggatca
    19441 gcgtacgcgg gcagtaatgg acaaatttag agcgtttgtc gaagaaggcg cagtcgtact
    19501 catccgcttc gataacaaag aagtcgcttt cgcccagacg cgccgaaacc tcaaagttcc
    19561 ccggcacacc gccgattaca aagcccggtt tgtaaccaca ctgttccaga atccaggtcg
    19621 ccattcccgc ggtggtggtt ttgccgtgtg taccggcaac ggccagcacc cagcggtcgc
    19681 gcagcacaaa atcgtgcagc cactgtggac ctgacatata agggatgttt ttttccagta
    19741 ccgcttccac acacggattt ccacgggtca tggcgttgcc aataatcacc agatccggct
    19801 gcgggtcgag ctggctggca tcgtacccct gaatcagctc aatgccttgc ttctcaagta
    19861 aggtgctcat cggcggatac acattggcgt ccgaacccgt tacttcatgg cctaactggc
    19921 gcgccagcat cgccagaccg cccataaacg tgccacaaat tcctaaaata tgaatgcgca
    19981 tgcgtcacta tcctttttta atctgccgct cattttacgc atatgtcggg caagtgagaa
    20041 acgcatttca ggataatccg taatttgctg gcgcgattca cctgagcgca acattccgat
    20101 tatttgttaa gattgttacg gtcgctttac tccataaaca ttgcagggaa agttttatga
    20161 aaacgttagg tgaatttatt gtcgaaaagc agcacgagtt ttctcatgct accggtgagc
    20221 tcactgcttt gctgtcggca ataaaactgg gcgccaagat tatccatcgc gatatcaaca
    20281 aagcaggact ggttgatatc ctgggtgcca gcggtgctga gaacgtgcag ggcgaggttc
    20341 agcaaaaact cgacttgttc gctaatgaaa aactgaaagc cgcactgaaa gcacgcgata
    20401 tcgttgcggg gattgcctct gaagaagaag atgagattgt cgtctttgaa ggctgtgaac
    20461 acgcaaaata cgtggtgctg atggaccccc tggatggctc gtccaacatc gatgttaacg
    20521 tctctgtcgg taccattttc tccatctacc gccgcgttac gcctgttggc acgccggtaa
    20581 cggaagaaga tttcctccag cctggtaaca aacaggttgc ggcaggttac gtggtatacg
    20641 gctcctctac catgctggtt tacaccaccg gatgcggtgt tcacgccttt acttacgatc
    20701 cttcgctggg cgttttctgc ctgtgccagg aacggatgcg cttcccggag aaaggcaaaa
    20761 cctactccat caacgaagga aactacatta agtttccgaa cggggtgaag aagtacatta
    20821 aattctgcca ggaagaagat aaatccacca accgcccgta tacctcacgt tatatcggtt
    20881 cactggttgc ggatttccac cgtaacctgc tgaaaggcgg tatttatctc tacccaagca
    20941 ccgccagcca cccggacggc aaactgcgtt tgctttatga gtgcaacccg atggcattcc
    21001 tggcggaaca agcgggcggt aaagcgagcg atggcaaaga gcgtattctg gatatcatcc
    21061 cggaaactct gcaccagcgc cgttcgttct ttgtcggcaa cgaccatatg gttgaagatg
    21121 tcgaacgctt tatccgtgag ttcccggacg cgtaatctcc tgcgcctctc cacacggaga
    21181 ggcgttttta ttgctgcgca atgttgactc ttgtcactgg cgaactctga cgattctccc
    21241 acagcaccgt taatccacgc tgtaatgcta taaaaataaa caacaaaata ccgatggcga
    21301 ttttcgtcca ccaggaactc agcgtgccat caaagtttat gtaagtctga atcagtccct
    21361 gaatcgccac gccaaaaagc gtccctaata ccgttccaac gccaccgctc aaaagcgtac
    21421 cgccaattac cactctgagg cgatagcatc cagttccaca cctacgcccg ccagcgcata
    21481 tccggcctgg gtataaatcg agaagacaat ccccgccagc gttgccagtc cggtggagag
    21541 catataaatg cgaatagtgg tgctgcgggt agaaatcccc atcaggtttg ccgacgttgc
    21601 gttgccgcca atggcgtata cctgattacc aaaacgggta cgatgcgcga ggaatatgcc
    21661 gataaccacc accgccagca tcagcagtcc catcgcactt aagcgaccgc cgccagggat
    21721 tttccacgca aggcttgaga gcgtgtcata aatgggatgg tttatcggaa tcgactcttc
    21781 cgaaacgaga tagctgacgc cgcgcaaaaa gaacatcccc gcaagcgtaa tgataaatgc
    21841 cgggatcttc agggcgtcga tcagaagccc cataaatgcg ccgaaggcac agcccatcac
    21901 cagcactagc ggaaacgcca gcagcggcga gaggccgaaa tcgccaacca cttttgccag
    21961 aaacacgcca gtaaaggcga tcaccgaacc gacggagaga tcgataccgc cagagaggat
    22021 cacaaagatc atgccaacgg cgattatccc aagaaaggcg ttatcggtca ggatattgca
    22081 gatcaccctt gtggaagcaa aaccgggaaa ctgggtcagg cagtagagat agcccagcac
    22141 aaagacgccg atggtgatca tcagcggcaa gttacgtttt atcatggcta cgtactcctt
    22201 taatcagact gataaagcgt tgcgactgaa caatcagcac acaaagcacc accaccgctt
    22261 tcaccacctg gttcatctcc ggcggaaagc ccgaaagcaa aattccggtg ttcatcccct
    22321 gaataatcag cgcccccact accgagagca gcaggttaaa gcgcccgccc atcagcgatc
    22381 cgccgccaat caccaccgcg agaatggcgt ccagctccag ccataatccg gcgttgttgg
    22441 catcggcacc gcgaatatcc gccgccacga taatgcccgc aatcgccgca catagcccgc
    22501 tcaacacgta ggtgagcatg acgatgattc gcgtgtttac cccggcattt tttgccgccc
    22561 gaatgttgat accaacggct tcgataaaca tccccagcgc cgttttgcgg gtcaacagcc
    22621 agaacaggag aagcgtcagc accgcgataa tgaccggcgt tggcaggaac aacagcgatc
    22681 cgctgccaaa ccatgagaga tccggcgagt taaacgtgac gatctgcccg gcggtgatca
    22741 gttgcgccac gccgcgcccg gcgaccatca ggatcagggt ggcgacaaac ggctgaattt
    22801 tgaggatcgc taccagtatg ccgttccaca atcccgccag gatgccggcg cccagggcgc
    22861 ttaacaaaac aatcggcagg ctgaatcccg cgaccgtcat cgcagccgtt gtggctccgg
    22921 cgatagccat caccgccccc accgagagat caatcccacc tgtcgcgatc accagcgtca
    22981 taccgatagc caacagcgca acgggagctg cacggttaag aatgtcgata gggctaccga
    23041 acaaacgccc atcctgaagc accacctgcc agaaatgcgg ggctaccagg ctatcgacca
    23101 gcagcaccag caacagtgcc gccagctgcg gcatccccgt cggccagtga aaacgccttt
    23161 ttggcggcgt agtgtcaggg agagattgag gcatcacact gttctcctta cgccgcaatg
    23221 gcgttcatga tcgccggaac ggaaagctct gccagcggga tctccgccac ctgtttgcga
    23281 tcgcgcatga tgatcacccg gtcggcatag cccaccagtt cttccagttc ggaggagatc
    23341 accagcagcg ccagaccatc ggcgcatagc gtttcaatca ggcggatgat ctcggcgtgg
    23401 gcaccaacat caatgccacg ggttggctca tcaaggatca gaaattgcag tcgggtcagt
    23461 agccaacgtg aaagcagcac tttttgctga ttgccaccgg agagaaattc aatcggttgt
    23521 tcagttgaag gtgtgcgaat gccaagctgg cggataaagc gttcggcaat ctcttgctgc
    23581 tctttgcggg aaatgggtcg tagccagccg cgctgggcct ggagagcgag gatgatattt
    23641 tcccgcaccg aggcggcggc gatgatgcca tcggttttcc tgtcttccgg gcagaatccg
    23701 atgcccagta ctgaagcctg atgtggcgat cgcagggttt gcagtttgcc tttgatcaac
    23761 gccgtgccgc tgtcagcagg tttgataccg aagatcactt cggcggtttc ggtacgtccg
    23821 gatcccagca agccagccag accgacgatc tcgccggggc gcacttcgag atcaaacggt
    23881 gctatcgttc cttttttgcc gtaatttttg aacgcggcaa cgggtttgtc gctcaacaat
    23941 gttcgcccgg cacgctgtag cgcgtgggta tccagctcgc gccccagcat catttttacc
    24001 agttcgatct gcggtagctc gcgcgtttcc cgacagccta cgaaactgcc gttgcgtaag
    24061 acagtgatcc gatcgctgac ctgatagacc tgatcgagaa agtgagtgac aaagatcagg
    24121 ctgacgccgc gatcgcgcaa ctgacgcatc aggccaaata atagctccac ttcctgggtg
    24181 tcgaggctgg cggtgggttc atcgaggatc agcactttgg cggagagatc gatagcccgg
    24241 caaatggcga cgatttgctg catcgccacc gaaaaacggt taagcggttc gcgcacgtcg
    24301 agggagaaac cgtaagatgc catcagttcg gtggcgcgct tttccatctc tttacggcgt
    24361 agaaggccga aacgtttggg ttcgcgacct atgaatagat tatcagcaac cgacatattg
    24421 ggtagcaggt tgacttcctg atagacggtg ccgataccga gttgttgtgc atgggcggta
    24481 tttttcggtg agatagcctg gccttccagc cagatggtgc cgcgatcggc gtggtataca
    24541 ccagttaatg ctttgattag cgttgatttt cccgccccgt tttcaccaag cagcgccatg
    24601 atttcgccac ggcgcaggct gaaatcaacg ttgtctaacg ctttgacgcc ggggaaaaat
    24661 ttacttaatc cttcggtgcg gaggatctcc tggtgttggt cggtcgtcat ggtttccccc
    24721 ggtgtaaagc gtgctcccag taggcatgat aagacgcggt aagcgtcgca tcaggcttca
    24781 gtgcaccatc gccggatgcg gcgcgagcgc cttatccggc ctacgaatcg gcatcaggca
    24841 ttgataccct caatacccca tatttttctt cttctctaac tcttcttttg cggtatcagg
    24901 cagataaagg gtggatttgg tcagcgttag cttttcaggc atggtgccgt cttttttgta
    24961 tttctccagc gcgtcgaagg cgggacctgc catattcggc gtcagttcaa cactggcgtt
    25021 cgcttcgcca tcaatcatcg ctttgtagat atccggtacg ccatcgatag aacctgtcag
    25081 aatatctttg cccggtttca ggcccgcttc tttaattgcc tgaattgcgc caatcaccat
    25141 gtcatcgtta tgggcgtaaa ccatgcagat gtttttgccg ttgttttccg ctttgataaa
    25201 gctttccatg acttctttgc ctttactgcg ggtaaagtca cctgactgcg agcggataat
    25261 tttgatattt ggcgcattct taatggcttc ggcaaagcct ttcttgcggt caatggcgac
    25321 gctggcccca acggtgccct gcagctccac cacgttgcat ggtttgccat tcacttcttt
    25381 taccagccag tcaccgatca acttaccttc gaggatgttg tcggcagtaa cggtggtcat
    25441 atagagagat ttgtctttca catcgatgga acggtcgagc aagaagaccg ggatttcggc
    25501 atctttcgcc tcttttaata ccggttccca acctgtcgcg accaccggag cgataaagat
    25561 cgcatccacc ccttgtgcaa cgaaggaacg taccgcttta atctggtttt cctgcttttg
    25621 ctgaccatcg gcaattttca acgtaattcc gcgtttttcg gcctcacttt tcgccacatt
    25681 ggtttctgca gcgcgccagc cggattccga tccgacctgc gaaaatccta cggttaatgg
    25741 agcggccaac gccatagacg acatggctgc cgagactgca gagactacaa gtaagcgttt
    25801 ccacataggg ttgtcctcgt cggggtaatt attattggtg aaaagatgtt cgcgaaaaaa
    25861 ctatagacaa ttcgttatgt aacggattgc gttacatcac acttcaaaga agtaaataaa
    25921 acgttaatca caagtttgta atcgctttca tctcactatg aaaaatgcgg ctacggttat
    25981 ggattttcct gctctgtata ccgtcttaaa attggcgaaa aaggaaaatg aagacgaaaa
    26041 caagcgaaga cattcggcgc gagttggcta taatactcgg cacttgtttg ccacatattt
    26101 ttaaaggaaa cagacatgag cttactcaac gtccctgcgg gtaaagatct gccggaagac
    26161 atctacgtag ttattgagat cccggctaac gcagatccga tcaaatacga aatcgacaaa
    26221 gagagcggcg cactgttcgt tgaccgcttc atgtccaccg cgatgttcta tccgtgcaac
    26281 tacggttaca tcaaccacac cctgtctctg gacggtgacc cggttgacgt actggtcccg
    26341 actccgtacc cgctgcagcc tggttctgtg atccgttgcc gtccggttgg cgttctgaaa
    26401 atgaccgacg aagccggtga agatgcgaaa ctggttgcgg ttccgcacag caagctgagc
    26461 aaagaatacg atcacattaa agacgtaaac gatctgccag agctgctgaa agcgcagatc
    26521 gctcacttct tcgagcacta caaagacctc gaaaaaggca agtgggtgaa agttgaaggt
    26581 tgggaaaacg cagaagccgc taaagctgaa atcgttgctt ccttcgagcg cgcaaagaat
    26641 aaataagttc ttctagcgca ataaccctga acgccgggct tcggttagta agggtttttt
    26701 tatgcccgcg ataaataaac tctctattcc accatcatta ttctcagcgg ttgcaaggct
    26761 tgaacggtaa gaacaagcaa acccgaccac cattttgctg ttcatagcca cttgctggaa
    26821 gttagccgac ctcactcata ctcaccgttc aaagcgtgcc cccgttttac ccctgcacat
    26881 ttgtgaacgc cagcagagtt ttttatgcga ttgcagatat ggaaagcggc tcggagagaa
    26941 aagcaaaaga tggaaaagtt acaaaaaaaa tcgcgagata catcgcataa aacaacgcca
    27001 cccgaaggtg gcgtgctttt attattactt atccctgtct aaccagtcgc cgctttcaat
    27061 tagctttaat ccatcgacgg gtcgttgata aacgtacatc catgcactcc cgtacggcgt
    27121 ctgaatcaac tggcgcgcgt attcaccgcc cctggtgcgc aaggcatcaa gttcggccag
    27181 cgtggcgttg tcaatacgat aaacttcacc gtgtaccgtt ccgttccccg gaactgcgcc
    27241 tggatagtgc cccaggctat acaactggta gttatcgata ctgaaatcgc ccagtaactg
    27301 ggcattggtc atccagtgac tgttgccttg tttgtggcgt aaactgccgt agacaaatat
    27361 tcgcattgct aaaactcgaa ctgatagagc aaatccagtg cctggtctac accagacacg
    27421 gcttccagat atagcttagg cattaggcga taacgtaacg tgagtgttgc tatagagtca
    27481 aatataccca cgccgtattt cacttgcaga cctggcaata catagccgct gaccactacc
    27541 tgggaggagt cgcctactcc ctgggtgtcg agcgctaaat tgcttacgcc aaacgtctcg
    27601 ccgattttac ccacaatctg gccactttgc gcaaccccca gaccaatcag catcgaggtc
    27661 attgccgcac tgtcgctctg atcgctctcc agcccttgtc cacgtagcaa ataagacaat
    27721 gcagcttgtt gcgacatcgc cgggtcagag aagatctccg ctttcggttc gtccgccaga
    27781 ccagtgacgc gaactccggc gattacgtcg tcttctgtag catccgggtt acgaatagct
    27841 tcaatattaa gatacggttg atccggcgga ccagagaaca gtaactcacc cttacgcaca
    27901 atcagatcct gaccataggc atggaagcgc ccttcaggga tgttgatctg cccgttcagg
    27961 cccagccctt gtttgtcctg tacgacgtta agatcgcccg tcagccgcgc tttcaggcca
    28021 aaggcgtcaa tgcgcacatt gttgccaacg tggacaatca ggttactgtt aatcggaatc
    28081 gacgccgttt tcggctcttc cggttgcagg ttatcgttaa gcatcaccac atcgctggag
    28141 acgcctactg cgctttccgg cagatcgtgc accacgatgc gcgcccacgg gacatccacg
    28201 cgaccatcga gggtaaacag gtttggtgta gcctcgaata caacatctgg cgatacatcc
    28261 attcgtacca tcggcggcac ggtgatccgc actttactgc ctttcgccgt tactcgtgcc
    28321 cgccagtttt caatttggct ccagtcggca tcaccgttca ggtagatttc accctgctgg
    28381 gtccgtactg taccggcaag cgtcgagcgc ataccgttaa agttgaccgc aagctggctc
    28441 ggctgcatat caaacggcat aaagttgccg tcgatatcca cacccgtaac ctgaagctga
    28501 ccaaacaact gcgggctttg cacatcacca cccagacgca agttggcact caccatcccc
    28561 gctgcttttt ccccacgggt aaagatgggg tttatcatcg caaggttgaa gttacggata
    28621 ttgacgttgc caccaagatt acggcggcct tgcggatcgg tcacctgcac ctgtccatca
    28681 aactggccgt tattggtcag gcggatggtc cagcccaatt cggcacggtt gttacgcaat
    28741 tccgccgtca gattcagtgt ctgaaacgcc accggcagcg ccgcatcgtt gacggtttgc
    28801 gttacctgca cgttacgccc cgaaagggtg atactgccct gcggcagccc ctctttcgtg
    28861 gtgtcccagg caacatccgc tttacccgtg aagataccgc tggcctgagt ggtttctggc
    28921 ataaacggtt tcagcatggc gaggtcgaag cggttgagat tcaccaccgc acgcccttcg
    28981 gcccccgcat cgatagtttg cggcacgcac agttccgcat tcgggttaag ccagcagtgt
    29041 ggcccgatgc tgattttttg ctccttattg cggtaatcca gcgcaatatc gcgggtcagc
    29101 gaccacgggc cgaccggcgt ctggaagcgg gtattgctaa gagttccctt ccagcgttct
    29161 tctttgcgat caaaacttcc tgccagatta agctgcccgg agacaggctc gccctgaatc
    29221 cgcaactgta gctcgtgctg cttttcgctg cctttggcat tcaaggtgac gaggttgata
    29281 tttacatccg gctgcgaaat ttgctcaacg cgtacgtcga gtttcccggc gatctgatcg
    29341 gtggatttaa tgtcgccttc cacgcgaacc tgcgccacgg aaagttcctg ccagcgcagg
    29401 ccgcgcgcgg tgatatctgc cagtagttgt ggcgcttcca ccgtgccgcg tactttcacc
    29461 agccctttcg ctgtaccgcc aagccccggc agcgcgttat ccagccccgg cgcgttgatg
    29521 gtggcatcaa gattgagatc ttttaccccc agctcgcctt tcacttcggc actgtttggc
    29581 ccgagttcca gatgaagccc agggatcatc cactgcatat aactgttgcc tttcagcgtg
    29641 ccgtcaacgt tcactttgtt ctgtttgacg ttaccggtca gcttcagttc tggcacctcc
    29701 atctgccagg tgccgccgta caggctaccg cgggttttaa tcaagccatt gagtttcgac
    29761 ggccactccg ggaactcttt ggcggtgtta atgccattaa gcgttagctc accgcgccaa
    29821 ctaatggcct gctgccagtc gagcaacgcc ttgagttcag ttttcccttc cagcgccgcg
    29881 acggtgagtt tgtcgagatt gacctgctgt tcattacctt tggcatcaag ggtaatggtg
    29941 gctggcggga tctcctgtcc cttcactgcc gtacgcattg agagcgtgta atcggtcatt
    30001 ttgccggtaa gtttcagttt cagatcatcc gcctgatact gcttctcacc agtgaacggc
    30061 cagtaaagct gtttgctgtt cacttccacg ttgagtggca atccggcttc cgccagtcgc
    30121 gtctgggcgc gtaaatccat atccaccgga ccggaaaggt taacgcctat ctccagctgt
    30181 tcgcgcagcg cgccgcccac tttcagcttc accttttcac ctttcaacgg ctccacgttc
    30241 agtgtgctgt tgagggtgat atccaccggc cagttgtctg acagctgcgc cgtgccgctg
    30301 gcgttgacga tcccctggtt ggaatcgata tccagggcgt ccagtttagt attgccgtca
    30361 atgctgctca ctttcagcag catggtgcgc acggtgatgt ccgtgtcgcc cgtcacgcgc
    30421 agctgctcgc ccttaaactc ttcaatgttc aggttaagcg gcaaatgcac gtcggtcatt
    30481 tccggcaata ccgggcgaga aaagagatct ttcagcgttt cgccgagcgg cttttcctcc
    30541 ggctgcggat tttcaatttt cggttcgacc acttcttcct gcgccacttc cgccacttcc
    30601 gccactttcg gcagagcaat cagcaggcct ttcagcgacg tcggtttcag ggtcagggtt
    30661 ttctcctgcc agttcaggcc ggaggtgaag tccatcaccg acaccgtggt gtcatcaatc
    30721 ttgatgttga cgttgtccag cgccacccgt gtcagggtga tgggatacgg cgtggagaga
    30781 tccagcggac cgctatcttc ctcttcttca acctgttcag aaggaggcat ttttttactg
    30841 tcgatgttga cctgaatgtc tttcagcgcc aggtcattaa tacaaacgct actgttccac
    30901 aggcactcaa gaccgaccgc cagatgcaga ttacccgctt ttaccgccac gcctggctgc
    30961 tcataacgaa cgtcagacaa ggtgagatcg cgccagccgc cggtgacctt gccaatatcc
    31021 agtcctggca cccagcgatc cgccgcttta aatacaagat gcaggccgct ggtggtgccc
    31081 accagaaacg ccaccgatcc cagcaacagt aagataacga taaccacgcc gaggctgatt
    31141 tttttccata aactcataat tctggcccca gaccgatgta aaactgtaac ccgtgttcgt
    31201 ctttatccgc gaccggtacg gcaaaatcga gtttgattgg cccgaccggc gattcccagc
    31261 gcacgccgac cccggtaccg gttttaaagt cgctgcggcg aatatcgctt accgcttcgc
    31321 cactatcgac aaacaccgcg ccccaccatt ttccggtcac gttgtactgg tattccagcg
    31381 atccggttat caacttcgag gcccctttca ggtcaccgtt ggcgtatttc ggggcgatag
    31441 atttgtattt gtagccgcga atactgcgat cgcccccggc gaagaaacgc agatccggcg
    31501 gtactttgtc gaaatcaccg gtttcaatcc agcccagcgt gccgcgtgta acgaaacgat
    31561 ggcgatcgta cagtgtgcgg atccagacgt tctgcgcctg gaaaacggag aaatcgacat
    31621 ctgaacccca ggccgtgttg gagtagtcga tagagtagcg ttgcgagtcg ccccaggttg
    31681 gcatcaggcc accacgagaa cgcgtgcggc taatcatcac cccaggataa aacagcatcg
    31741 tggtgttggt aatttcaccc tgagtaaagt ggtcgagact ccagcgcagg ttaatggcac
    31801 gctgccagcc gctggagaga tcccagtagc gagaagccac cagcgtagtg gagtcagact
    31861 cggtatcgtt caggtcagtg cgcttaaaac cgccctgcac caaataatat tgttccagtg
    31921 gattcttcag cagcggcatt ttatagctga agtcgagggt ctgttccggc gcggaaatac
    31981 tggtactggt ggtcagactg tgaccataag agttcatcca cggctttttc cacgtcgctt
    32041 tcacgcgcgg tcccacgtcc gtagagtaac cgaccccggt ttcgatggtg ttttctgttc
    32101 gcggcgaaac cacgcccgtc aatggtaata ctttcgtttc gcgcgcttta tcaaattgtg
    32161 gagccactac gaccgagtta aaccagccgg tagcagaaag tcgacggttc agttccgcca
    32221 gatctttcga ttcgtactca tcgccctctt taaacggcac cagattttgc aggtattcat
    32281 cgcggatttg tgatccttca aaggtcacat gcccaaagcg gtaacgttcg ccactgttat
    32341 aatcaatatc ccagaaggct ttatgcaggc cgagcgtaat gcccagctgc gctttggtaa
    32401 attcgctatc gaaataacct ttacgcaacg caatgctggt taaggacttt ttgaaatttt
    32461 cataatcgcc ctggttcagt accgtgccaa tagccgggcg agtatcgagc aatttcaaat
    32521 agtctttatc ggtccgcgcg ccgccgcgca ataccacatc ggtgccgcca attaacaccg
    32581 gcacgcctgg cgtgactttg gcgatcaata cctgccgccc tttctttggc ggtggacgga
    32641 gatcaaattc aatggtcggc tggtaataac ccagcgcttt cagaccttca cggatggcat
    32701 catcgacgcg tgcgcgaaag cgacggtctg gcgtcacttc atcactttca atcgtagaaa
    32761 gctgcgcacg aacgttcttt tccagctgtc ccgataaccc ctcgacctgt agacggacgt
    32821 tcgcggcgac ggcagatccg cttaagcaga gtaagcttac acagcataac tgtcggatat
    32881 agcgcacatt ttctcctgaa tatccttttt tcctgcccct ggaacgccgt taaacggctt
    32941 aacaaaaatc cagtaatatg gattaaaaag cagactaaac cccaaatatt tcttatgttt
    33001 tactttagac ctattcacgg tggttattgt gtgcaaatac gcctcttgtt acaaccttaa
    33061 ccccaatgac cgattttcgg gagagcgaca ccatgagttt atttgataaa aagcatctgg
    33121 tttcccccgc cgatgccctg cctggacgta acaccccgat gcccgtagcc acgctgcatg
    33181 cggtcaacgg tcactcaatg accaatgtac ctgacggaat ggaaattgcc atttttgcga
    33241 tgggttgttt ctggggtgtg gagcgtctgt tctggcagtt acccggcgtt tacagcaccg
    33301 ccgcaggcta taccggcggc tatacgccaa atccgactta tcgggaagtg tgctccggtg
    33361 atacgggtca tgccgaagcg gtacgcattg tttacgatcc tgccgtcatc agctatgagc
    33421 agttgctaca ggtattttgg gagaatcacg atcccgccca gggaatgcgt cagggcaatg
    33481 accacggcac gcagtatcgt tcagcgattt atccgctgac cccagaacag gatgccgcag
    33541 ctcgcgccag tctggaacgt tttcaggcgg cgatgcttgc cgccgatgat gatcgtcaca
    33601 tcaccacgga aatcgctaac gccacaccgt tttattatgc cgaagatgac caccagcaat
    33661 atctgcataa aaacccgtat ggttactgtg gaattggcgg aattggcgtc tgtctgccac
    33721 cggaagcata gcgttacggg tacaaatgta gattgttgat aaagtgcgct ttatttatgc
    33781 cagatgcggc gtaaacgcct taccaggcct acaaaatcgt ccaaattcaa tatattgcaa
    33841 ggactgcgta ggcctgattg gcatagcgca tcaggcagta ttgcatttat cagcagtctg
    33901 aattttaacc ctctggcgac tttacagtac cttacgctat actagccact gaaaatgccg
    33961 gttcactttc ttcgaatcgg ctttcaatgt gtatttcaca caaattaatc aacttcccct
    34021 tccgaggatc tggcctgaaa ggtcggataa gatatgttaa acagtatttt agtcatactc
    34081 tgcttgatcg ctgtaagtgc gttcttctcg atgtccgaga tctcgcttgc cgcctcacgc
    34141 aaaatcaaac ttaaactgct ggctgatgaa ggcaatataa atgcccaacg cgttctgaat
    34201 atgcaggaaa atcccggcat gttctttacc gtggtccaaa tcggtctgaa cgcagtggcg
    34261 attctcggcg gtatcgtcgg tgatgcggca ttttctccag cttttcacag cctgttctcc
    34321 cgctatatgt cggcagagct ctctgagcaa ctgagcttta ttctctcttt ctcgttagtg
    34381 actggcatgt ttatcctgtt tgcggattta accccgaaac gcatcggtat gattgcgcca
    34441 gaagcggtgg ctttgcgtat catcaacccg atgcgcttct gcctgtacgt ttgcaccccg
    34501 ctggtgtggt tcttcaacgg cctggcgaac atgatcttcc gtattttcaa actgccaatg
    34561 gtacgtaaag atgacatcac ttctgatgac atctacgcgg tagtggaagc cggtgcgctg
    34621 gcgggcgtgt tacgtaaaca ggaacacgag ctgattgaaa acgtctttga gctggaatcc
    34681 cgtaccgttc cgtcttcaat gacaccgcgt gaaaacgtga tttggtttga tctccacgaa
    34741 gatgagcaaa gcctgaagaa taaggtggcg gaacatccgc actctaagtt cctcgtctgt
    34801 aatgaagata ttgaccacat catcggttat gtcgattcta aagacctgct gaaccgcgtg
    34861 ctggctaacc aaagcctggc actgaacagc ggcgtacaaa ttcgcaacac gctgattgtg
    34921 ccggatacgt taaccctttc agaggcgttg gaaagtttta aaaccgcagg tgaagacttc
    34981 gcggtgatca tgaacgagta cgcgctggtg gtggggatca tcaccctcaa cgacgtgatg
    35041 accacgctga tgggcgatct ggtcggtcag gggctggaag agcagattgt cgcccgtgat
    35101 gagaactcat ggctgattga cggcggcact ccgattgacg acgtcatgcg cgtgctggat
    35161 attgacgagt tcccgcagtc gggcaactac gaaaccatcg gcggctttat gatgtttatg
    35221 ctgcgtaaga tcccgaaacg caccgattcg gtgaaattcg ccggctacaa atttgaagtg
    35281 gtggatatcg ataactaccg catcgaccag ctgctggtga cccggatcga cagcaaggcc
    35341 accgcccttt cgccaaaact gcctgacgct aaagataaag ataaagataa agaagaaagc
    35401 gtcgcgtaac ccccagaaac atcaacggct cctgaatcag gagccgtctt attactgcat
    35461 agcactttgg ttaagccatc tctgtttgca gacgcataac ctgacggtta acttcggaca
    35521 tcactgacaa atgcagctta tccttcactt ttgggataag aatcttaccc ttatcaaatt
    35581 caaaagcgcc aacgtccttg atgtataacc gtccacggaa caggatcttt acgtacttcg
    35641 ccacctgaag tgggttgtac cgttggaaaa ttttcattgt tatctcctgc tgagtattac
    35701 gcctgtgcgg tgccacaatc ggcccaacta ttatgaggcg caaattttaa tgcttagtga
    35761 ctatagacta tccgggcaat gtttccaccg cgtataactt tttttaccta aaggttacaa
    35821 ttattcagaa ttatcttttt accgaagcgc ggtcttcagt ataagcattc atttttcata
    35881 tgatttgtgc gcttgaccgc aaactggcat cacacttgcg ggaaattcga taaatagcac
    35941 atatgattaa aactcagacc caagtggtcg gatcacctgc atatcataag aaggaaacac
    36001 catgacccta cgcaagattc tggcactcac ctgcctgctg ttgccgatga tggcttccgc
    36061 acatcagttc gaaaccggtc agcgagtgcc gccgattggc attaccgatc ggggcgagtt
    36121 ggtgcttgat aaagatcagt ttagctacaa aacctggaac agcgcgcagt tagtgggaaa
    36181 agtgcgagta ctgcaacata ttgctggtcg cacctctgca aaagagaaaa acgtgacgct
    36241 gattgaagcg attaaatcag caaagttacc gcacgatcgt taccagacca ccaccattgt
    36301 taacaccgac gacgcaattc cgggttccgg catgtttgtg cgcagcagtc tggagagtaa
    36361 taaaaagctt tatccctggt cgcagtttat tgtcgatagc aatggcgtcg cgcgcggtgc
    36421 ctggcagctg gatgaagaaa gttccgctgt agtggtgctg gataaggatg gtcgcgtgca
    36481 atgggccaaa gacggggcgc tcacacagga agaggtgcag caagtgatgg acctgctgca
    36541 aaagttgctc aaataatggc aataacataa attctgcaaa aataattttc ccctatcgtt
    36601 atctatggga taggggaatt aggtctctga gctcttacac cgaactcata ctggtaacgc
    36661 tagttgctct tttttaaacg cctcaatggt tgatttatat ttctcgcaaa tctcctcttc
    36721 ggtaatatca ctatctacgg ccagattcgc acttactatc gcatccactt gttcggctaa
    36781 ttcgacttgt tggcaacctg cattaaccag aaaattataa ataagagagt aatcttcatc
    36841 ctccaaaaca acagaggaac cattacgaaa acgttcatcc cctccataga ttttaagcgc
    36901 aaagcccaaa gattctatga ctatggaaaa attatcatca attatttttt cgtaaatata
    36961 ccatccatcg cattctttaa ttccacctcc tggtatttgg agttcaaatt taccccgttt
    37021 taatttgctt actatatcct ccaataaggc ataacctaaa gacacgtcac caatcgcctg
    37081 gtgaatataa cctttaatcc ttttcggtat gtcagactgt tttttctgca gaatatccca
    37141 ggcatacaat cttctgtttt gttctaacgg aaattcacgg tttttgaaat ccatgaatga
    37201 gttatactca tttattgtta tggggatttc ttcgtattca aatccactgc ctcgcaatgt
    37261 cattgtatat tcggcaccgt tatctttaag gctgaaacta ataagaaact tatccatggc
    37321 tttaacagtt tcattttttg atgaaagcac ttgataacta tctttatcaa tctgagttgc
//
LOCUS       DAZKHC020000039        37214 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZKHC020000039 DAZKHC020000000
VERSION     DAZKHC020000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 37214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.039"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(241..1833)
                     /gene="lsrK"
                     /locus_tag="ACHOSM_RS11375"
                     /old_locus_tag="pgaptmp_002275"
     CDS             complement(241..1833)
                     /gene="lsrK"
                     /locus_tag="ACHOSM_RS11375"
                     /old_locus_tag="pgaptmp_002275"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HGU4558789.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     RQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSIAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1912..2865)
                     /gene="lsrR"
                     /locus_tag="ACHOSM_RS11380"
                     /old_locus_tag="pgaptmp_002276"
     CDS             complement(1912..2865)
                     /gene="lsrR"
                     /locus_tag="ACHOSM_RS11380"
                     /old_locus_tag="pgaptmp_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HGU4558790.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIAHTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3114..4649
                     /gene="lsrA"
                     /locus_tag="ACHOSM_RS11385"
                     /old_locus_tag="pgaptmp_002277"
     CDS             3114..4649
                     /gene="lsrA"
                     /locus_tag="ACHOSM_RS11385"
                     /old_locus_tag="pgaptmp_002277"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HGU4558791.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYVRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNHPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4643..5671
                     /gene="lsrC"
                     /locus_tag="ACHOSM_RS11390"
                     /old_locus_tag="pgaptmp_002278"
     CDS             4643..5671
                     /gene="lsrC"
                     /locus_tag="ACHOSM_RS11390"
                     /old_locus_tag="pgaptmp_002278"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HGU4558792.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQAARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5671..6663
                     /gene="lsrD"
                     /locus_tag="ACHOSM_RS11395"
                     /old_locus_tag="pgaptmp_002279"
     CDS             5671..6663
                     /gene="lsrD"
                     /locus_tag="ACHOSM_RS11395"
                     /old_locus_tag="pgaptmp_002279"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HGU4558793.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6675..7697
                     /gene="lsrB"
                     /locus_tag="ACHOSM_RS11400"
                     /old_locus_tag="pgaptmp_002280"
     CDS             6675..7697
                     /gene="lsrB"
                     /locus_tag="ACHOSM_RS11400"
                     /old_locus_tag="pgaptmp_002280"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HGU4558794.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAIQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7724..8599
                     /gene="lsrF"
                     /locus_tag="ACHOSM_RS11405"
                     /old_locus_tag="pgaptmp_002281"
     CDS             7724..8599
                     /gene="lsrF"
                     /locus_tag="ACHOSM_RS11405"
                     /old_locus_tag="pgaptmp_002281"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HGU4558795.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8623..8913
                     /gene="lsrG"
                     /locus_tag="ACHOSM_RS11410"
                     /old_locus_tag="pgaptmp_002282"
     CDS             8623..8913
                     /gene="lsrG"
                     /locus_tag="ACHOSM_RS11410"
                     /old_locus_tag="pgaptmp_002282"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HGU4558796.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8970..9728
                     /gene="tam"
                     /locus_tag="ACHOSM_RS11415"
                     /old_locus_tag="pgaptmp_002283"
     CDS             8970..9728
                     /gene="tam"
                     /locus_tag="ACHOSM_RS11415"
                     /old_locus_tag="pgaptmp_002283"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HGU4558797.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALRDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9732..10646)
                     /gene="yneE"
                     /locus_tag="ACHOSM_RS11420"
                     /old_locus_tag="pgaptmp_002284"
     CDS             complement(9732..10646)
                     /gene="yneE"
                     /locus_tag="ACHOSM_RS11420"
                     /old_locus_tag="pgaptmp_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HGU4558798.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10853..12304)
                     /gene="uxaB"
                     /locus_tag="ACHOSM_RS11425"
                     /old_locus_tag="pgaptmp_002285"
     CDS             complement(10853..12304)
                     /gene="uxaB"
                     /locus_tag="ACHOSM_RS11425"
                     /old_locus_tag="pgaptmp_002285"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HGU4558799.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQEDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRLLC"
     gene            complement(12531..13948)
                     /locus_tag="ACHOSM_RS11430"
                     /old_locus_tag="pgaptmp_002286"
                     /pseudo
     CDS             complement(12531..13948)
                     /locus_tag="ACHOSM_RS11430"
                     /old_locus_tag="pgaptmp_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(14087..14446)
                     /gene="yneG"
                     /locus_tag="ACHOSM_RS11435"
                     /old_locus_tag="pgaptmp_002287"
     CDS             complement(14087..14446)
                     /gene="yneG"
                     /locus_tag="ACHOSM_RS11435"
                     /old_locus_tag="pgaptmp_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HGU4558800.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14446..15372)
                     /gene="glsB"
                     /locus_tag="ACHOSM_RS11440"
                     /old_locus_tag="pgaptmp_002288"
     CDS             complement(14446..15372)
                     /gene="glsB"
                     /locus_tag="ACHOSM_RS11440"
                     /old_locus_tag="pgaptmp_002288"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HGU4558801.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15436..16824)
                     /gene="sad"
                     /locus_tag="ACHOSM_RS11445"
                     /old_locus_tag="pgaptmp_002289"
     CDS             complement(15436..16824)
                     /gene="sad"
                     /locus_tag="ACHOSM_RS11445"
                     /old_locus_tag="pgaptmp_002289"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HGU4558802.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMVQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16925..17806
                     /gene="yneJ"
                     /locus_tag="ACHOSM_RS11450"
                     /old_locus_tag="pgaptmp_002290"
     CDS             16925..17806
                     /gene="yneJ"
                     /locus_tag="ACHOSM_RS11450"
                     /old_locus_tag="pgaptmp_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4558803.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17883..18998
                     /gene="yneK"
                     /locus_tag="ACHOSM_RS11455"
                     /old_locus_tag="pgaptmp_002291"
     CDS             17883..18998
                     /gene="yneK"
                     /locus_tag="ACHOSM_RS11455"
                     /old_locus_tag="pgaptmp_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HGU4558804.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLNPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMINPGVPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSK
                     VHRWFVNKDPNLNTAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            19148..20338
                     /gene="ydeA"
                     /locus_tag="ACHOSM_RS11460"
                     /old_locus_tag="pgaptmp_002292"
     CDS             19148..20338
                     /gene="ydeA"
                     /locus_tag="ACHOSM_RS11460"
                     /old_locus_tag="pgaptmp_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HGU4558805.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20363..21028)
                     /gene="marC"
                     /locus_tag="ACHOSM_RS11465"
                     /old_locus_tag="pgaptmp_002293"
     CDS             complement(20363..21028)
                     /gene="marC"
                     /locus_tag="ACHOSM_RS11465"
                     /old_locus_tag="pgaptmp_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HGU4558806.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            21240..21674
                     /gene="marR"
                     /locus_tag="ACHOSM_RS11470"
                     /old_locus_tag="pgaptmp_002294"
     CDS             21240..21674
                     /gene="marR"
                     /locus_tag="ACHOSM_RS11470"
                     /old_locus_tag="pgaptmp_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HGU4558807.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVKLKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21694..22077
                     /gene="marA"
                     /locus_tag="ACHOSM_RS11475"
                     /old_locus_tag="pgaptmp_002295"
     CDS             21694..22077
                     /gene="marA"
                     /locus_tag="ACHOSM_RS11475"
                     /old_locus_tag="pgaptmp_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HGU4558808.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMSNMQGESRFLHPLNHYNS"
     gene            22109..22327
                     /gene="marB"
                     /locus_tag="ACHOSM_RS11480"
                     /old_locus_tag="pgaptmp_002296"
     CDS             22109..22327
                     /gene="marB"
                     /locus_tag="ACHOSM_RS11480"
                     /old_locus_tag="pgaptmp_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HGU4558809.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(22358..23257)
                     /gene="eamA"
                     /locus_tag="ACHOSM_RS11485"
                     /old_locus_tag="pgaptmp_002297"
     CDS             complement(22358..23257)
                     /gene="eamA"
                     /locus_tag="ACHOSM_RS11485"
                     /old_locus_tag="pgaptmp_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HGU4558810.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            23452..24639
                     /gene="ydeE"
                     /locus_tag="ACHOSM_RS11490"
                     /old_locus_tag="pgaptmp_002298"
     CDS             23452..24639
                     /gene="ydeE"
                     /locus_tag="ACHOSM_RS11490"
                     /old_locus_tag="pgaptmp_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HGU4558811.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSARSLGWLGAAINPLVSGVVLTNLPPSSLFIILALVIIAAWVLMLKGIRARPWRQP
                     ALC"
     gene            24766..24861
                     /gene="mgtS"
                     /locus_tag="ACHOSM_RS11495"
                     /old_locus_tag="pgaptmp_002299"
     CDS             24766..24861
                     /gene="mgtS"
                     /locus_tag="ACHOSM_RS11495"
                     /old_locus_tag="pgaptmp_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HGU4558812.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            24861..24965
                     /gene="mgtT"
                     /locus_tag="ACHOSM_RS11500"
                     /old_locus_tag="pgaptmp_002300"
     CDS             24861..24965
                     /gene="mgtT"
                     /locus_tag="ACHOSM_RS11500"
                     /old_locus_tag="pgaptmp_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HGU4558813.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(25080..25970)
                     /gene="dgcZ"
                     /locus_tag="ACHOSM_RS11505"
                     /old_locus_tag="pgaptmp_002301"
     CDS             complement(25080..25970)
                     /gene="dgcZ"
                     /locus_tag="ACHOSM_RS11505"
                     /old_locus_tag="pgaptmp_002301"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HGU4558814.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELILAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMFLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(26225..26617)
                     /gene="ydeI"
                     /locus_tag="ACHOSM_RS11510"
                     /old_locus_tag="pgaptmp_002302"
     CDS             complement(26225..26617)
                     /gene="ydeI"
                     /locus_tag="ACHOSM_RS11510"
                     /old_locus_tag="pgaptmp_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HGU4558815.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            26893..27411
                     /gene="ydeJ"
                     /locus_tag="ACHOSM_RS11515"
                     /old_locus_tag="pgaptmp_002303"
     CDS             26893..27411
                     /gene="ydeJ"
                     /locus_tag="ACHOSM_RS11515"
                     /old_locus_tag="pgaptmp_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HGU4558816.1"
                     /translation="MNINRDKIVQLVDTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(27455..29500)
                     /gene="dcp"
                     /locus_tag="ACHOSM_RS11520"
                     /old_locus_tag="pgaptmp_002304"
     CDS             complement(27455..29500)
                     /gene="dcp"
                     /locus_tag="ACHOSM_RS11520"
                     /old_locus_tag="pgaptmp_002304"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HGU4558817.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSKDLEHLYRQWRGKAPQIMPMLQHRGLNI"
     gene            29637..30383
                     /gene="ydfG"
                     /locus_tag="ACHOSM_RS11525"
                     /old_locus_tag="pgaptmp_002305"
     CDS             29637..30383
                     /gene="ydfG"
                     /locus_tag="ACHOSM_RS11525"
                     /old_locus_tag="pgaptmp_002305"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HGU4558818.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            30472..31158
                     /gene="rspR"
                     /locus_tag="ACHOSM_RS11530"
                     /old_locus_tag="pgaptmp_002306"
                     /gene_synonym="ydfH"
     CDS             30472..31158
                     /gene="rspR"
                     /locus_tag="ACHOSM_RS11530"
                     /old_locus_tag="pgaptmp_002306"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HGU4558819.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            31336..31539
                     /gene="ydfZ"
                     /locus_tag="ACHOSM_RS11535"
                     /old_locus_tag="pgaptmp_002307"
     CDS             31336..31539
                     /gene="ydfZ"
                     /locus_tag="ACHOSM_RS11535"
                     /old_locus_tag="pgaptmp_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HGU4558820.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(31623..33035)
                     /gene="ydfI"
                     /locus_tag="ACHOSM_RS11540"
                     /old_locus_tag="pgaptmp_002308"
     CDS             complement(31623..33035)
                     /gene="ydfI"
                     /locus_tag="ACHOSM_RS11540"
                     /old_locus_tag="pgaptmp_002308"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGU4558821.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLS"
     gene            complement(33124..34407)
                     /gene="ydfJ"
                     /locus_tag="ACHOSM_RS11545"
                     /old_locus_tag="pgaptmp_002309"
                     /pseudo
     CDS             complement(33124..34407)
                     /gene="ydfJ"
                     /locus_tag="ACHOSM_RS11545"
                     /old_locus_tag="pgaptmp_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            34539..34781
                     /locus_tag="ACHOSM_RS11550"
                     /old_locus_tag="pgaptmp_002310"
     CDS             34539..34781
                     /locus_tag="ACHOSM_RS11550"
                     /old_locus_tag="pgaptmp_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4558822.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(34890..35047)
                     /locus_tag="ACHOSM_RS11555"
                     /old_locus_tag="pgaptmp_002311"
                     /pseudo
     CDS             complement(34890..35047)
                     /locus_tag="ACHOSM_RS11555"
                     /old_locus_tag="pgaptmp_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            35376..36026
                     /gene="nleG7"
                     /locus_tag="ACHOSM_RS11560"
                     /old_locus_tag="pgaptmp_002312"
     CDS             35376..36026
                     /gene="nleG7"
                     /locus_tag="ACHOSM_RS11560"
                     /old_locus_tag="pgaptmp_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HGU4558823.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGVFVKNALNSNICTL
                     YDKSAFMNLTREHLPHPLSREKIVKEMIIERNMCYFDTISQHFIIMDADQQKQHCK"
     gene            complement(36251..37126)
                     /locus_tag="ACHOSM_RS11565"
                     /old_locus_tag="pgaptmp_002313"
     CDS             complement(36251..37126)
                     /locus_tag="ACHOSM_RS11565"
                     /old_locus_tag="pgaptmp_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HGU4558824.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTPGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARALFGQ
                     KRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDIE"
BASE COUNT         9703 a         9272 c         8674 g         9565 t
ORIGIN      
        1 aaagaacttt attaaaactc attttcattc atttatgcat ttaggaatat tcgctataaa
       61 ataagggtta tgcttaatgc tttaagaaaa atagcaattt tcccttgaat atagtactcg
      121 tgatggaagg atgaatctgc aagctggcat ttaacaagcc agctctaaaa gaagggaaat
      181 aagaataact atactcaaaa actaacagcc acggtcatca tgatgtggct gtcaatgaaa
      241 ctataaccca ggcgctttcc ataacgacgt cgtcagtcca tgatcaacca gccccagctg
      301 atcctgataa actgcctgcc acttatcgcg tgaatcctga taaagttcat gcttttccgg
      361 gtctggtgtg tgcgtccgtt cccagcgaac caggcgttct ccggtttctg ctattgatga
      421 aaaaattccg gcaccgacgc cagctgcaat ggcacatcct aatgcggtgg cttctttgac
      481 taccggaata ttgacgggta atcccgagac atcagcgaga atttgactcc ataatttccc
      541 ttttgaacct ccgccagcaa aaaccagcga ggtgggatga atattcgaga aatcagcaat
      601 ttgctgcaag ttacacgctg atacaatcgc cgcattttct tccagcgcac gaaacaatgt
      661 cgctttgtta catttatccg ggtcaatgga caagttaata aaggaaggtg cggcgtgata
      721 ccaggtttta aagcgcattc tgtcggagaa gatcggcatt acgccccacg acccaggcgg
      781 cacccgactg gccatctctt ccagcagcgt ataggtgtcg atgcctaaac gttcggcaat
      841 cagtttttct tcggcacaga aagcatcgcg gaaccagcgc atggtgagtc cggtaaaaaa
      901 gcttatagat tcagcttgta ccatgccagg aataacatga ggattaacgc gcacgttcat
      961 ttctgggtct gtcaccggcg cggctaaatt tacaacttgc tgccggaatg tgccgccaag
     1021 aaccgcggtt tgtgccggac gcacaacgcc taacccaagg caaccaagct gcacgtcgcc
     1081 tcctccaacg accaccggag tgcccgcctt cagaccgcag agttccgccg cttgtgaact
     1141 taccacgccc agcaatgtgc cggtttcttt gacaggagaa agaatatcgg cacgcaggcc
     1201 agccatatcc agcaatgcag gtttccagtc acgggtggtt agatcaagaa gtcccgtggt
     1261 gccagcgtta gagggatcca ccgccagttc gccgctaagc atataggcca gccagtcgct
     1321 gatcatggtg atggttgatg cctgacggta aatatcggaa cgatggtgcg ccagccaaag
     1381 taatctgggg atggcactta aagccagtgt ttgtccggtc gcgcgataaa cttcgttttc
     1441 aaaggtattg ttgtgcagtt ctttaagttc gctaacttcg cgcgctgctc tggcatccac
     1501 attggcgcag gcccagatcg gtgttccttc attgttatat aaaacaatgc cttcacgcat
     1561 cgaacatgcc gaaacggcag cgatatactc cgggtctatg ccggcgttgt gcagcgcctg
     1621 gcgcatacac tcacacgcca gttgccagtt tttgttgaga tcaaattcca tagaaccagg
     1681 aacgtccggt actgccaggt gccgccactc cgcctgtccc actgctattt gattgccttc
     1741 caggtcgaat atcacagccc gaatacttcc ggtacctgca tccagcgcca tcaggtaata
     1801 ctttgattct gaaggggtaa agagtcgagc cattagatta tcctcggctt attcgttctg
     1861 tctgttcctc tatacgttct ccatcattcc cggtaataag gtcatgcaaa tttaactacg
     1921 taaaatcgcc gctgctgtgt cctgatcggt aaccagtgcg ttgatataac cgcctttcat
     1981 tgcagcggca attgcttcgg ctttattttc tccccctgcc acgccaaccc ggacgggtat
     2041 ggtcttcagc gcgcttaaag gtaagccaat cagttcgtta tgtattttaa tatccgtgac
     2101 aacgtcacct tttgcatcaa aaaagtatcc taaaatgtcg ccaaccgccc cttttcgacc
     2161 aatcattaac tgttcgccct ggctgatata accggagcga atgatcgtcg cgtcgtcctg
     2221 ttgactgacg gccccaatgc cgacaatcgc cacatcggct gcttgcgcgg ctaacagaac
     2281 atctttgacg caattttcat tttttagcgt atgggcaatg tcagcggagg atgcccgcaa
     2341 cggagccgga ataatattca cactgcacgc cgcgttaagc tgcccgatac ccgtcatata
     2401 agaaccgacg ccaccggaga gcgtgaccag gcgaatttgc tgtgacgaaa taaaaccact
     2461 taagcgttgc agcgtattca tggttgcctc gccaaaacca atcgccagca tctgttgtgg
     2521 ttgaagtaaa ctcatcaaca tatgcgccgc gcctatcccc agtcgcccgc cgacatcagc
     2581 atccgcaagc ccagggatca cccggacatg ttgcagcgaa aactgacgac gtaattgtgt
     2641 ttcatattcc agacagcctt caaagcgaga attaatctgc acgcgaataa tgccggactg
     2701 atgccctttc tccagtaatc gcgacacttt caaacgggtc aggccgagac gatcgctgat
     2761 ctcactctgg gtcagcccgt cgtggtagta aaaccacgcg atccgcgcga cctgttcttc
     2821 ttcacacatt ccctgttctg aaattgccga atcgttgatt gtcataattc attcttcact
     2881 ttgaacatat ttaaatcttt aatgcaattg ttcagttctt gcgcatttat atctgtgatg
     2941 gcaaccacag tttgactcta cgagcatgaa caaacgcaac cgtgaaaatc aaaatcgcat
     3001 aaattgtgat ctattcgtcg gaaatatgtg caatgcccac ctaaggttat gaacaaatta
     3061 aaagcagaaa tacatttgtt caaaactcac ctgcaaaact gaacggggga aatatgcaaa
     3121 cgagtgatac ccgcgcgtta ccgcttcttt gcgcccgctc ggtttataaa cagtattcag
     3181 gggtcaatgt cctgaaaggc atcgatttta cgttgcatca gggggaggtc cacgccctgc
     3241 tcggcggcaa tggtgccggt aaatcgacgt taatgaagat tattgccggt attacccctg
     3301 ctgatagcgg tacgctggag attgggggca acaactacgt cagattaacg ccagttcatg
     3361 ctcatcagct gggtatttat ctcgttcccc aggaaccgct gcttttccca agcctgtcga
     3421 taaaagaaaa catcctgttt gggctggcaa aaaaacagct ctccatgcag aaaatgaaga
     3481 acttgctggc ggcgctgggc tgccagtttg atctgcatag tctggcagga tcgctggatg
     3541 tcgccgatcg ccaaatggtg gaaatcctcc gcgggctgat gcgcgactcg cggattctga
     3601 tcctcgatga acctaccgcc tcgcttaccc ctgcggaaac cgaacgcttg tttagtcgct
     3661 tgcaagagct gcttgctact ggcgtgggta ttgtttttat ctcgcataag ctgccggaaa
     3721 ttcgccagat tgccgatcga attagcgtga tgcgcgacgg aaccatcgcc ttaagcggca
     3781 aaaccagcga actgtctacc gacgacatta ttcaggccat tacgccagcg gtacgggaaa
     3841 aatcgctctc tgccagccaa aaattatggc tggagttacc tggcaaccgc ccacaacatg
     3901 ccgccggaac gtcggtgctg acactagaaa atctgaccgg cgaaggtttc aggaatgtca
     3961 gcctgacgct caatgccgga gaaattctgg gcctggctgg gctggtggga gccggacgca
     4021 cagaactggc cgagacgctc tatggtctgc gtactttgcg tggcggacgc attatgctga
     4081 atggtaaaga gatcaataaa ttatccactg gagaacgttt actgcgcggt ctggtttatc
     4141 tgccggaaga tcgccagtca tccggactga atctcgatgc ttcgctggcc tggaacgtct
     4201 gcgcccttac tcataacctt cgtggattct gggcgaaaac cgcgaaagat aatgccaccc
     4261 tggaacgtta tcgtcgggcg ctgaatatta aatttaacca cccggaacaa gctgcacgga
     4321 cattatccgg tggcaaccag caaaaaatcc tcattgccaa atgcctggaa gcttcgccgc
     4381 aagcattgat tgtcgatgag ccgacgcgcg gcgtggatgt ctcggcccgt aatgatatct
     4441 accagctgtt gcgcagcatc gccgcacaga atgtggcagt gctgcttatc tcctctgatc
     4501 tggaagagat cgaactgatg gcagatcgtg tgtatgtgat gcatcagggc gaaattaccc
     4561 actctgcact gaccgggcgc gatattaatg tcgagactat tatgcgcgtt gccttcggcg
     4621 atagtcagcg tcaggaggcg tcatgctgaa gtttattcag aacaaccgtg aaatcacggc
     4681 actgctggcg gtggtgctgc tgtttgtatt acccggtttt ctcgaccgcc agtatttaag
     4741 tgtgcaaacg ctgaccatgg tttatagcag cgcgcaaatc ctgatcctgc tggcaatggg
     4801 cgcgacgctg gtaatgctta cgcgcaatat tgatgtttca gtgggttcga ttaccggaat
     4861 gtgcgcagtg ctgttgggga tgttactgaa cgcggggtat tcactacctg ttgcttgtgt
     4921 cgcgacttta ctgcttggtt tgctcgcggg atttttcaac ggtgtcctgg tcgcgtggct
     4981 aaaaatccct gccattgttg ccacccttgg cacgttaggg ttgtacagag gcatcatgtt
     5041 gctgtggact ggcggtaaat ggattgaagg gttacccgcc gaactgaaac agctctccgc
     5101 cccgctgctg cttggcgttt ccgcgattgg ttggttgacg ataattctgg tggcatttat
     5161 ggcctggctg ctggcaaaga cggcgtttgg ccgcagtttt tatgccacgg gcgataattt
     5221 acaggccgct cgtcaactgg gtgttcgtac ggaagccatt cgcattgtgg cattttcgtt
     5281 gaacggctgc atggcggcac tggcgggaat tgtgtttgct tcgcagattg gttttatccc
     5341 caaccagacc ggtaccgggc tggagatgaa agcaattgca gcctgcgtgc tgggcggcat
     5401 tagtttgctc ggtggttccg gtgcgatcat tggtgcggta ctcggcgcat ggttcctgac
     5461 gcagatcgat agcgtactgg tgctgttgcg cattccggca tggtggaatg attttatcgc
     5521 gggtctggtt ctgctggcgg tgctggtgtt tgatggacgc ctgcgttgtg cgctggaacg
     5581 taatctacgg cggcaaaaat atgcccgctt tatgacgcca ccgccatccg ttaaacccgc
     5641 ttcgtcaggt aaaaaacggg aggccgcata atgcgtattc gctacggttg ggaactggct
     5701 cttgccgcac tgctcgttat tgagattgtc gcatttggtg caattaaccc gcgaatgtta
     5761 gatctcaata tgttgctgtt cagcaccagt gactttatct gcattggcat tgtcgcccta
     5821 ccgctgacga tggtgattgt cagtggcggg atcgatattt cgtttggttc gaccatcggc
     5881 ctctgcgcca ttgcattggg cgtactgttt caaagtggtg tgccgatgcc gctggcgata
     5941 ctcctgacct tactgctcgg cgcattgtgc gggctgatca acgccggatt aattatctat
     6001 accaaagtta acccgctggt gattacgctt ggcacgctgt atctgtttgc cggaagcgct
     6061 ctgctgcttt ccggtatggc cggagcgacg gggtacgaag gtattggtgg attcccgatg
     6121 gcgtttacag atttcgctaa cctggatgtg ctgggactcc ccgttccgct gattatcttc
     6181 ctgatatgtc tcctcgtttt ctggctctgg ctgcataaaa cccatgccgg acgtaatgtg
     6241 tttttgattg ggcaaagccc gcgcgtggcg ctttatagcg cgattccagt taaccgtacc
     6301 ttatgtgcgc tctatgccat gacgggtctg gcatctgcgg tcgccgctgt gctgctggta
     6361 tcgtattttg gttcagcacg ttccgatctc ggtgcgtcat ttctgatgcc cgccatcacc
     6421 gccgtggtgc tcggcggtgc caatatttat ggtggttccg gttccattat cggcaccgcc
     6481 attgcggttt tattagtggg atatttgcaa caaggtttgc aaatggcagg agtgccaaat
     6541 caggtgtcca gcgccctttc cggtgcgcta cttatcgtcg ttgtcgtagg tcgttccgtt
     6601 agcctgcatc gccagcaaat taaagagtgg ctggcgcgtc gggccaataa cccattgcca
     6661 taaaggatat cttcatgaca cttcatcgct ttaagaaaat cgccttactt agcgctcttg
     6721 gcattgccgc aatctctatg aatgtgcagg ccgcagagcg tattgcattt attcccaaac
     6781 tggttggcgt gggatttttt accagcggtg gcaacggcgc acaacaagcg ggtaaagagc
     6841 tgggcgttga tgtgacctac gacgggccga cagaacccag tgtttctggt caggtacagt
     6901 tgattaataa cttcgtcaat caaggttata acgccattat cgtttctgcg gtttcgcctg
     6961 atggcttgtg tccggcactg aaacgcgcca tacaacgtgg tgtgagagtg ctgacctggg
     7021 actctgatac taaaccggag tgccgctctt actacattaa tcagggaacg cccgcccagt
     7081 tgggaggtat gttggtggat atggcggcgc gtcaggtgaa taaagacaaa gccaaagtcg
     7141 cgtttttcta ctcaagcccc accgttacgg accaaaacca gtgggtgaaa gaagcgaaag
     7201 cgaaaatcgc caaagagcat cctggctggg aaattgtcac tacgcagttt ggctataacg
     7261 atgccactaa atcattacaa accgcagaag gaatattaaa agcgtatagc gatctcgacg
     7321 ccattatcgc ccccgacgcc aacgccctgc ccgctgccgc acaagccgca gaaaacctga
     7381 aaaatgacaa agttgcgatt gtcggattca gtacgccaaa cgtgatgcgt ccatatgtgg
     7441 aacgcggcac ggtgaaagaa tttggcctgt gggatgtggt tcagcaaggc aaaatttcag
     7501 tgtatgtcgc ggatgcatta ttgaaaaaag gatcaatgaa aacgggcgac aagctggata
     7561 tccagggcgt aggtcaggtg gaagtctcgc caaacagcgt tcagggctat gactacgaag
     7621 cggatggtaa tggcatcgta ctgttaccgg agcgcgtgat attcaacaaa gagaatatcg
     7681 gcaaatacga tttctgatgt gcattactta accggagtaa gttatggcag atttagatga
     7741 tattaaagat ggtaaagatt ttcgtaccga tcaaccgcaa caaaatatcc cttttatcct
     7801 gaaaggttgc ggtgcgctgg attggggaat gcagtcacgc ttatcgcgga tatttaatcc
     7861 gaaaacgggt aaaaccgtga tgctggcctt tgaccatggt tattttcagg ggccgactac
     7921 cggacttgaa cgcattgata taaatatcgc cccgctgttt gaacatgccg atgtattaat
     7981 gtgtacgcgc ggcattttgc gcagcgtagt tcccccggcg accaataagc cggtggtact
     8041 gcgggcgtca ggtgcgaact ctattctggc ggaattaagt aatgaagccg tggcgttatc
     8101 gatggatgat gccgtgcgcc tgaacagttg cgcggtggcg gcgcaggttt atatcggcag
     8161 cgaatatgaa catcagtcga tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt
     8221 gggaatgccg accatggccg tgactggcgt gggcaaagac atggtgcgcg atcagcgtta
     8281 tttctcgctc gcgactcgaa tcgccgctga aatgggggcg caaattatca aaacctatta
     8341 tgtcgaaaaa ggttttgaac ggattgttgc cggatgtccg gtacccattg ttattgctgg
     8401 cggtaaaaaa ttaccggagc gcgaggcgct ggaaatgtgc tggcaggcta tcgatcaggg
     8461 cgcttctggt gtggatatgg ggcgtaatat tttccagtct gaccatccgg tggcgatgat
     8521 gaaagccgta caggcggtgg ttcaccataa cgaaacggct gatcgggcat atgaactcta
     8581 tctgagtgaa aaacagtaac tgcggatcta aggagaagaa ttatgcacgt cacactggtt
     8641 gaaattaacg ttcatgaaga caaggttgac gagtttatcg aagtttttcg ccagaaccac
     8701 ctaggctctg tacaggaaga aggcaatttg cgcttcgatg tcttacagga cccggaagtg
     8761 aattcgcgct tttatatcta cgaagcctat aaagatgaag acgcagtggc gttccataaa
     8821 accacccccc actacaaaac ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt
     8881 aaaaaacgtc tgttcaatgg gttgatgccg tgaggcgaat tcatcagttt tatctacaat
     8941 tggggtaacg cgctgacggg agtaaataaa tgtctgactg gaacccctct ttatatctgc
     9001 acttttccgc tgaacgatcg cgtccggcgg tggagctgct tgccagagtg ccgctggaga
     9061 atatcaaata cgttgccgat cttggctgtg gcccaggtaa cagcaccgcc ctgctgcatc
     9121 aacgttggcc tgcggccagg ataacaggca tcgactcgtc tccggcgatg attgctgaag
     9181 cgcgcagtgc tttgcgagac tgccagttcg tggaagcaga catccgcaac tggcaaccgg
     9241 aacaggcact cgatctgatt tttgctaatg cctcactgca atggctgccc gaccactacg
     9301 aattgtttcc tcacttggtt tctttactta atccgcaggg cgtgctggca gtacagatgc
     9361 cagataactg gctggagccg acccatgtgc tcatgcgcga agttgcctgg gaacaaaact
     9421 acccggatcg cgggcgtgag ccgttggctg gcgttcatgc ttactacgat attttgagcg
     9481 aagccggatg tgaggtcgat atctggcgaa ccacctacta tcaccagatg ccgtcgcacc
     9541 aggcgattat cgattgggtg accgccactg gattacgtcc gtggttacag gatctgaccg
     9601 agagcgaaca gcagcttttt cttacgcgct accatcagat gctggaagag cagtatccac
     9661 tgcaagagaa cggacagata ctgctggcat ttccgcgtct gtttattgtt gcccgccgta
     9721 cggagtaatt atcacgtcag ctggtaatga cgatcgggaa gaatttttgc tggaatttct
     9781 gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt tgcagatggc atccagcggt
     9841 aaatcattgt tttcagtacc gaacggatct tccagttctt ccgccagaca atccagcgaa
     9901 ataaaagtgt aggaaatcag cacagagata aaaggcgtca tgtaatgcag gtccacgacc
     9961 agcgcgaacg gcagcatgat acaaaacaga taaacggtac gatgcaaaat cagggtgtag
    10021 gcaaagggaa ttggcgtgta ggcaatgcgc tcgcagcctg ccagaaccgc tgaaatatca
    10081 ttaagccgat cgttgaggct aataaacagg atatctgaaa gctgtccatt gcggcgctga
    10141 accgccaacc actcccccat tattaacaag atacggttgg ctggagagtt cgaagccagt
    10201 acaggctgaa gatcttcagt cttgagataa tgagccagca cttccgcctg tggctgtttg
    10261 cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca gccgggcaaa ctcccttaca
    10321 cttgccgaat ccggcaatgt cgtttttacc tcgcgcagta acgaccgtga ggcaatcatc
    10381 aactgccccc aaagttttcg cgcttcaacg taacgggcgt acccggcatt attacgaaaa
    10441 ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg gtgcgagggt gaatttgatg
    10501 cccagatgcg tgtaccaggg cagcatgaaa ataacagcga tagaaaagag aaaattgagt
    10561 agtaagcgcg atgatatctt ggataatact gagccgtgcc agacaaaaat acggcgcagc
    10621 cagtgttgtt gtggacgaac aatcatggtt atcttcaggc gtgaaaaagt cgccctatta
    10681 aacgtgatta cgatcacatt ctcaagacgc tacttacaaa ttacctacaa gcttacaaca
    10741 ttaacaggcg ctgcatgtgg catctgttaa tgataaaggc acataggatg tagtaactaa
    10801 tatagtgaaa taaaaaaccc ggtcgaaacc gggttcagaa gtaacggtgt tattagcaca
    10861 acaggcgtac agcttcgcgc atcccttttt cgagaatcgc atccaggtca ttagccacct
    10921 gctcgaccag accaggcact tgcgtcaggt cctgctccca gtggtctttt tctgccaaca
    10981 caatcgctac cagttcctgg gtaccgataa cacggtcacg atgctggctc cagagttgct
    11041 gataacgttc cagccagtgt gcatcctcct gtaccggata tgtttctccg ttacgctcac
    11101 cgcgatagaa cgcaatcaat gccgctaatg cgaaagtaag gcgcgccgga agtgtgccgt
    11161 ttgccttctg ccctgccagc agctgcggca ggatgcgggt gcggaacttg gtcataccgt
    11221 tgagcgcgat agacagcagc tgatgcttaa tgtacgggtt acggaaacgc ccggtgactg
    11281 cactggcgaa agattccagt tcatcacgag gcaaatccag taccggaata atttcttcgt
    11341 agatggcttt ttcaacgaat gcgcaaattt cagcatcgtt catcgcttca cctacggtat
    11401 ccagccctgc ctggaacgcc accggcacca gcgcggtgtg cgcaccgttg aggattgcca
    11461 ctttgcgttc tttatacggt ttaatatcgt caacaatcag cacgttgagc ggatatttgt
    11521 ccaggcgcag ctcagtcgct aaggatttcg gcccctgaat cacaaacagg taaaagtgtt
    11581 cagcggtgtc gaggaaacca tcgtgataac ccagttcttc ttccagttta gccacttcat
    11641 cgcgcggata accggtaacg atacggtcca ccagcgtaga acagaagctg ttagcctgat
    11701 ccagccattg aataaatgct tctggcagtg cccactcttg cgcatagcgc agcaccagtt
    11761 cacgcaacgc gtcgccattg tagtcaatca actcacacgg aatgatgatc caacctttat
    11821 ccagcgcacc gttgaaatgg ctaaagcgtt cgaacagcag acgggtcagt tttgccggat
    11881 agcttaccgc tggcgcgtca tcgaatttat cgcccgcatg gtagctgata cccgcttcgg
    11941 tggtgttaga gaaaacaaag cgcatttccg ggttgtgcgc cagtttcagg aattcatcgt
    12001 attcactgta gacgctgatt tcacgattaa ccgagcgaat cagacgcgcg tcgctgaccg
    12061 cttccccctt ctcattcagg ccacggataa tggtggtgta cagaccatcc tgcgtgctca
    12121 gtgacggcgg gaatgaagtt tcaatcggac gaacaacgac cacgccagaa ttcagatcgg
    12181 tgtgctcatt caggagatcg atttgccagt caacaaaggc gcgcaggaag ttaccttcac
    12241 caaactgaat gatacgttct ggatactgtg caccgggaaa atcgcgacga tttagtgttt
    12301 tcacaatggg ctcccttctg attggtcata caacctgttt taattggtac gacaggttag
    12361 caaactttaa tacgccgacc ccctgttttg atcaactcct gatgattaat gagcagtttt
    12421 atgagaaaag tgtggcgcgg atcatggttt aatcgaggaa atacaccttt tcctggatca
    12481 taaagtggtg gaacacattg cattcaaatc gcgcggaatg aataaagatg tcagacaact
    12541 tcctcaccgt aacgcatagt gctggtacgg ttgcgcccat ctttcttcga gcgatacaga
    12601 cacgtatcgg ctcgaccatc aatttattaa aatcatcggt cagcattcgg tacgatgcgc
    12661 gaccactacc gacgccaata cttaccgtga gataaagcgt tttttgttgc caggtgaatg
    12721 gttgcagttc aacgccttta cggatttttt ccgccattag cagaccatcg acaggattca
    12781 ccgacggcac tgcaacagca aattcttcgc cgcccatgcg cgccaccagc cccttatcac
    12841 cgacaatctt ctgaatatgc cgggcaaaca cgcttaacac tttatcgcca cattcatgtc
    12901 catagttatc gttaatgctt ttgaaatagt cgatatcaag cagcatgacc gtcagatgtt
    12961 gtgtctgttt cagcgatgga cttttcagcg cctcataaag accggaccgt gagtaaacct
    13021 gagtcagaaa atcaaagtcg gctcgcagcg caacttgctt cattagcgaa ttgatcgctg
    13081 ccacgctaaa agaaaccata attgggcata tcgccatcgt ggcaataccg agacgtgcgg
    13141 agaacatttc cggaatggag aacggcgaac cgaccgagat atcaatcacc gaatttgcca
    13201 ccagtacgat ttccaccgca ccggtgacaa aggtcaacag acatgttacc tgcggcgtat
    13261 agcgcactgc acaccagatt aatgcaggca gcgggaacgc cagactcccc gctccgccaa
    13321 tgactaccga ggcaataacc gacacaataa gcgcgatggc gggcatcatc tgttctgctt
    13381 taaagcgcgg cagcactcca ggaatagcca acgtcagcat acaaggcacg atcaacacgc
    13441 ccgttgagaa ttgctcactg aaccagtccg caagcaaagg ccagaaatcc agactatcaa
    13501 tactgaccga accaatcgcc ccgacaatag cgcataataa tgcggcaatc agacagtaat
    13561 taaatagccg taaggcactt actggctcat acttattttt ccccagacgc ttatcccgca
    13621 cgaccagtaa ggcgacggta acaataaaca tcatattgga gaaattgata accagtgaaa
    13681 ccagtcccca ttcggtggtt atggcatcat aaaccagcat cgccacataa ctgatcgcat
    13741 agtaatgcag tcgattaagc cagacatagc gggcaaacac ccccgccatt acgccattca
    13801 gcggccagaa aagagaaagt tcgtgaacca gccgtaactc tgcaccaata aaatagaaca
    13861 gcgtggttag cgcaaaaata gcgatgctat tacgtagcag atgaccttcc tggaacaaac
    13921 ggaaagttga aatggggtgt acatgcatta attatctgtc catacaattg cgggtggcaa
    13981 ttatctcaac gtaataagta aattctcata aaggatagca tgttcgctaa cagcggcggc
    14041 aaataaaggt accctgggaa tgctatttta tcgttttccg caattctcat ggctgattca
    14101 tctgtacaac cagccagtga tagattaccg ccacgatata acgttgttgt tcttcgctta
    14161 acgaaacacc ctgcggaatg ttatgccact tagccagaca accgcgacag caggtcgcgg
    14221 tggcgtgctg agcgataaac accggatgcc cgcgcatggg ggtttgctta ccgtcattag
    14281 ccggtaatgc cggagcaaga cgtttagcga caaaatccgc cgcatgttgt tcgatgactg
    14341 gcgcgccttt ctccaggcaa tactgacgct ctttcatgcc cagacgaaag cgagagcgaa
    14401 attttgaacg ggataaacgc gcgaagagtg gatcgagaga ctgcattaat aaaccgaacg
    14461 ccctaactgt ttcgtcaatt gttcaaggac ggcgataccc gcaagcgagt tacctgcatc
    14521 atccagttcc ggactccaga cagcgatggc catttcatgc ggaacaatcg ccacaatacc
    14581 gccaccaacg ccagatttcg ccggtagccc cacccgccag gcaaactccc ccgcgttctg
    14641 gtacatacca ctggtcgcca tcagcgcgtt aatttgccgc gcctgcattg gcgtcaccac
    14701 aggttcatca atatgaatag ctttcccctg attagccaga aagacaaacg tccgggccag
    14761 ctctacacag ctcattttca gagcgcagta atgaaagtag ttttgcagaa cggttgtcac
    14821 gtcatgatgg aaattgccaa acgacttcat cagccaggcg atagccgcat ttcgcgcgga
    14881 atgttcaaat tcggaacgcg ctaccaccgt atcgtatgaa atatcagaca caccgcttaa
    14941 gccgcgcacg acttccagca tacgttgccg tggtgcgctt aatcgccctt gcaacatatc
    15001 gcaaaccacc agcgcaccag cattaataaa cggattacgc ggaataccct gctccatttc
    15061 cagttgcact aaagaattga acggtgatcc agacggatct ttgccgacgc gttgccagat
    15121 ttcctcttcg gagtaatgac gcatggcgac aacgagactc agcactttgg aaatagactg
    15181 aatggaaaaa cgttcttgcg cgtctccggc ctgaaaaagc tgtccgtcaa cggtacagat
    15241 agcaatgccc aatcgggaac cgtctactgt agccagcgcc ggaatataat ccgcgacttt
    15301 accctgacca atgagcggcc gcacttgccg caagatgttt tctaaaattg cattatccat
    15361 ggcgactgcc actttctact cctggaccgc aggtctgaaa agacctgcga gtatatcaga
    15421 gctgaatatg tcgcgtcaga tccggtcttt ccacaccgtc tggatattac agaattcgtg
    15481 taagccgaaa tgggaaagct cacgaccaaa gccactcttt ttcacgccac caaaggccac
    15541 tcgcgcgtcg ctggcacaat aaccattgat aaacacccca ccgcattcca gacgtgccgc
    15601 catctgtctg gcctgtgttt cgtcggtggt aaaaatggtc gctgaaaggc cgaactcact
    15661 atcattagcc agttccagcg catgttctgc atctttcgca acggtgattg ccgcaacagg
    15721 gccaaacatt tcttcccgaa acgcggtcat ttctggggta acattcgcca gaaccgttgg
    15781 cggatagtag ttacctgccc cagccatctt ttccccgccc agtaacaaac gcgcaccctg
    15841 cgccagggtt ttctccacct gatgatgcag ctcatcacgt aaatcaaaac gagccattgg
    15901 tccgagagcg ttctcttcat cacggggatc gcccattttc aaggctgccg cagctgccac
    15961 aaaacgttcg gtaaatgcag aagcaattcc ctcttcgata ataaagcgtt ttgccgctgc
    16021 acaaacctgt ccggtattct gataacgtcc ggctaccgcc gctttaaccg ccagttccag
    16081 atcggcatcg ttaagcacaa taaatggatc cgaaccgccc agttccagta cgcatttttt
    16141 cagtgccgct ccagcctgtg cgccaatagc cgctcccgca cgaacacttc cggtcaccgt
    16201 gacagcagca atgcgcgaat ctttaatcat ttgactgaca ccgtcgttgt cggcattcag
    16261 ccagccatat acgccttgcg ggattcccgc atctttaaac acctgggcaa tgagctgagc
    16321 acagcccatc acattcggcg catgtttaag taagtagccg ttacctgcaa gaatgatggg
    16381 aaccgcgcca cgcatcacct gccataacgg aaagttccac ggcataatcg ccagaatcgt
    16441 acccaacggt cgatactcaa taactgcctg ctgattttcc accagcgtag gttccgcctt
    16501 cagcattgcc ggaccatgtt ctgcatacca gtcacacaaa ttcgccgatt tcgccacttc
    16561 agcgcgcgcc tgattgattg gtttgcccat ttcacgggtg atcatttgca ccatttcttc
    16621 gctacgggcg cgcagagcct taccgatacc acgcagtttt tcagcacgat aatctatatt
    16681 tgtctcgcgc cagtcgcgaa agcctgctgc cgccagctga agtgcgtttt cgatatcgtt
    16741 agcgccagcc cacggcagca cagaaagttg ttcacccgtg gcaggattta tcgaaattgc
    16801 atgagttgcc ggagtaatgg tcatcggggt atctccttta tgagtcatgg tatgaagata
    16861 cgcagattta ctcttgcttt aaaatgaata atattaagcc acttattcac gaatcgagaa
    16921 tattatggat ctgacccaac tggagatgtt caacgcggtt gccgaagctg gcagcataac
    16981 ccaggcagca gcaatagtgc atcgcgtgcc gtcgaatctc actacccgtc tacgccagct
    17041 ggaaacagaa ctgggggtag atctgtttat tcgcgagaat cagcgtttac gtctctctcc
    17101 tgccgggcat aactttttac gctatagcca acaaattctc gcgttagtgg atgaagcgcg
    17161 gagcgttgtc gctggcgatg agccgcaagg tttattttct cttggttcgc tggaaagcac
    17221 cgcagcagtg cgcattccgg ccacgctggc ggaattcaac catcgttatc ccaaaattca
    17281 gttttcactt tccaccggcc cttccggcac tatgctggaa ggagtgctgg agggaaaact
    17341 gaatgcggca tttattgatg ggcccattaa ccatactgcc attgacggga taccggtata
    17401 ccgcgaggag ctgatgatcg tcacgccaca aggtcatgcg ccagtaatcc gcgccagtca
    17461 ggttaacggc agtaacattt atgccttccg cgccaattgt tcgtatcgtc gccacttcga
    17521 gagctggttt catgctgacg gtgccgctcc gggaactatt cacgagatgg agtcgtatca
    17581 cgggatgttg gcctgtgtgg tcgcaggagc aggcattgcg cttattccac gctctatgct
    17641 ggaaagtatg ccggggcatc accaggttga agcgtggccg ttagctgagc aatggcgttg
    17701 gttaacaacc tggctggtct ggcgtcgtgg tgcgaaaacg cgtccgcttg aggcatttat
    17761 tcaactactg gatgtgcctg actcggcaaa acagggttac caatgagcta ttttgatagt
    17821 tcttgcggct aatatgctct atatagtgat gttccgatga cttatgacta tatggggcaa
    17881 atatggttac gccagtaagc atcagcaatt acatatctct tcctgatgat tttcccgttc
    17941 gtaatattgc accacaggta aaagaagttt taaaggactt tattgatgcg ctaagtacaa
    18001 taatatgtga tgaagagtgg cgcacgtctt taaacatcaa ctcagccaca aaaaagatat
    18061 ttaataacct tgataactta tcatatattc agaggacttc tttccggggt aacgacacgc
    18121 tatacaatga aaaggttcag tttaaactca cttatcccgt caagaatgga agacacaaag
    18181 aaaatattga atttcaggta gtaataaatt taaaccctat ttatttagat aattttcgcc
    18241 atgatggaga aattaatatt ttttgcgctc ccaacccaaa gcctgtcact atggggcgcg
    18301 tatttcagac cggcgtcgag cgtgtgctct ttctgtttct gaatgatttt attgaacaat
    18361 ttccgatgat caaccctggc gttcccatca aaagagcgca tacaccacat attgaacccc
    18421 tgcctccgga tcaccatacc gctgcagatt acttacgcca gtttgatttg cttgtcctga
    18481 attttatctc tcgcggtaat tttgtcatac tcccccgatt atggaataac tctaaggttc
    18541 acagatggtt cgtcaataaa gatcctaact tgaacaccgc cattctcgac ataacggaca
    18601 gcgaattaaa agaggattta ttgcaaagcc tgatggattc attaggttct aacaaacatg
    18661 tactacccga agtctgcatc tgctttttat cccttttagc agaacaagaa tctcctcatt
    18721 ttcaggactt gtttttattt ttcgccaata tgttattgca ctatcaccaa tttatgaatc
    18781 ccaatgaaag tgatttgaat gacgtgttga tgccagcatc attaagtgat gataaaatta
    18841 tcaaacatat ggcgcgcagg accctcaaac tttttgtaaa aaatgaaacg ccgccaaaag
    18901 ttactcacga agacctggtg aaaaacaggc ctcgctcccc tgtcagacca cctatacccg
    18961 caacctccaa aacgccagac ctccctgaac gtcattaaac cgtgatgtta ccgactctct
    19021 gacgcgtgaa agaatcagcg tcagagaaac ggaaaacgcg atccagatca caaatgcatt
    19081 gtattcacat cattaaccgt tttaagatca tttcatcact ttttcgcaac ttacccgata
    19141 atctgttatg acaacaaaca ctgtttcccg caaagtggcg tggctacggg tcgttacgct
    19201 ggcagtcgcc gccttcatct tcaacaccac cgaatttgtc cctgttggcc tgctctctga
    19261 cattgcgcaa agttttcaca tgcaaaccgc tcaggtcggc attatgttga ccatttatgc
    19321 atgggtagtg gcgctaatgt cattgccttt tatgttaatg accagccagg ttgaacggcg
    19381 caaattactg atctgcctgt ttgtggtgtt tattgccagc catgtactgt cgtttttatc
    19441 gtggagcttt accgttctgg tgatcagtcg cattggtgtg gcttttgcgc atgcaatttt
    19501 ctggtcgatt acggcgtctc tggcgatccg tatggctccg gccgggaagc gagcacaggc
    19561 attgagttta attgccaccg gtacagcact ggcgatggtc ttaggtttac ctctcgggcg
    19621 cattgtgggc cagtatttcg gttggcgaat gaccttcttc gcgattggta ttggggcgct
    19681 tatcaccctt ttgtgcctga ttaagttact tcccttactg cccagtgagc attccggttc
    19741 attgaaaagc ctcccgctat tgttccgccg cccggcattg atgagcattt atttgttaac
    19801 tgtggtggtt gtcaccgccc attacacggc atacagctat atcgagcctt ttgtgcaaaa
    19861 cattgcggga ttcagcgcca actttgccac ggcattactg ttattactcg gtggtgcggg
    19921 cattattggc agcgtaattt tcggtaaact gggtaatcag tatgcgtctg cgttggtaag
    19981 tacggcgatt gcgctgttgc tggtgtgtct ggcactgctg ctacctgcgg cgaacagtga
    20041 aatacacctc ggggtgctga gtattttctg ggggatcgcg atgatgatca tcgggcttgg
    20101 tatgcaggtt aaagtgctgg cgctggcacc agatgctacc gacgtcgcga tggcgctatt
    20161 ctccggcata tttaatattg gaatcggggc gggtgcgttg gtaggtaatc aggtgagttt
    20221 gcactggtca atgtcgatga ttggttatgt gggcgcggtg cctgcttttg ccgcattaat
    20281 atggtcaatc attatatttc gccgctggcc agtgacactc gaagaacaga cgcaatagtt
    20341 gaaaggccca ttcgggcctt ttttaatggt acgttttaat gatttccagg atgccgttaa
    20401 taataaactg cacacccata cataccagca ggaatcccat cagacgggag atcgcttcaa
    20461 tgccaccctt gcccaccagc cgcataattg cgccggagct gcgtaggctt ccccacaaaa
    20521 taaccgccac caggaaaaag atcagcggcg gcgcaaccat cagtacccaa tcagcgaagg
    20581 ttgaactctg acgcactgtg gacgccgagc taataatcat cgctatggtt cccggaccag
    20641 cggtacttgg cattgctagc ggcacaaagg caatattggc actgggttca tcttccagct
    20701 cttccgactt gcttttcgcc tccggtgaat caatcgcttt ctgttgcgga aagagcatcc
    20761 gaaaaccgat aaacgcgacg attaagccgc ctgcaattcg cagaccggga atcgaaatgc
    20821 caaatgtatc catcaccagt tgcccggcgt aatacgccac catcatgatg gcaaatacgt
    20881 acaccgaggc catcaacgac tgacgattac gttcggcact gttcatgttg cctgccaggc
    20941 caagaaataa cgcgacagtt gttaatgggt tagctaacgg cagtaacacc accagcccca
    21001 ggccaattgc tttaaacaaa tctaacattg gtggttgtta tcctgtgtat ctgggttatc
    21061 agcgaaaagt ataaggggta aacaaggata aagtgtcact ctttagctaa ccttgcatcg
    21121 cattgaccaa aacttgaacc gatttagcaa aacgtggcat cggtcaattc atttatttga
    21181 cttatacttg cctgggcaat attatcccct gcaactaatt acttgccagg gcaactaatg
    21241 tgaaaagtac cagcgatctg ttcaatgaaa ttattccatt gggtcgctta atccatatgg
    21301 ttaatcagaa gaaagatcgt ctgcttaacg agtatctgtc tccgctggat attaccgcgg
    21361 cacagtttaa ggtgctctgc tctatccgct gcgcggcgtg tattactccg gttaaactga
    21421 aaaaagtgtt gtctgtcgac cttggagcac tgacccgtat gctggatcgc ctggtctgta
    21481 aaggctgggt tgaaaggttg ccgaacccga atgataagcg cggcgtactg gtaaaactta
    21541 ccaccagcgg cgcggcaata tgcgaacaat gccatcaatt agttggccag gacctgcatc
    21601 aagaattaac aaaaaacctg acggcggacg aagtggcaac acttgagcat ttgcttaaga
    21661 aagtcctgcc gtaaacaaaa aagaggtatg acgatgtcca gacgcaatac tgacgctatt
    21721 accattcata gcattttgga ctggatcgag gacaacctgg aatcgccact gtcactggag
    21781 aaagtgtcag agcgttcagg ttactctaaa tggcacctgc aacggatgtt taaaaaagaa
    21841 accggtcatt cattaggcca atacatccgc agccgtaaga tgacggaaat cgcgcaaaag
    21901 ctgaaggaaa gtaacgagcc gatactctat ctggcagaac gatatggctt cgagtcgcaa
    21961 caaactctga cccgaacctt caaaaattac tttgatgttc cgccgcataa ataccggatg
    22021 agcaatatgc agggcgaatc gcgcttttta catccattaa atcattacaa cagctagttg
    22081 ataacgtgac aacgtcactg aggcaatcat gaaaccactt tcatccgcaa tagcagctgc
    22141 gcttattctc ttttccgcgc agggcgttgc ggaacaaacc acgcagccag ttgttacttc
    22201 ttgtgccaat gtcgtggttg ttcccccatc gcaggaacaa ccaccgtttg atttaaatca
    22261 catgggtact ggcagtgata agtcggatgc gctcggcgtg ccctattata atcaacacgc
    22321 tatgtagttt gttctggccc cgacatctcg gggcttatta acttcccacc tttaccgctt
    22381 tacgccaccg caagccaaat acattgatat acagcccggt cataatgagc accgcaccta
    22441 aaaattgcag acccgttaag cgttcatcca acaatagtgc cgcacttgcc agtccgacta
    22501 cgggcaccag taacgataac ggtgcaaccc gccaggtttc atagcgtccc agtaacgtcc
    22561 cccagatccc ataaccaaca attgtcgcca caaacgccag atacatcagc gacaagatgg
    22621 tggtcatatc aatagtaacc agactgtgaa tcatggatgc ggaaccatcg agaatcagcg
    22681 aggcaacaaa gaagggaatg attgggatta aagcgctcca gattaccagc gacatcaccg
    22741 ccggacgcgt tgagtgcgac atgatctttt tattgaagat gttgccacac gcccaactaa
    22801 atgctgcagc cagggtcaac ataaagccga gcatcgccac atgctgaccg ttcagactat
    22861 cttcgattaa caccagtacg ccaaaaatcg ctaaggcgat ccccgccaat tgtttgccat
    22921 gcaatcgctc cccgaaagta aacgcgccaa gcacgatagt aaaaaacgcc tgtgcctgta
    22981 acaccagcga agccagtcca gcaggcatac caaagttaat ggcgcagaac agaaaggcaa
    23041 actgcgcaaa actgatggtt aatccatacc ccaagagcaa attcagtggt actttcggtc
    23101 gtgcaacaaa aaagatggcc ggaaaagcga ccagcataaa gcgcaaaccg gccagcatca
    23161 gcggtggcat gttatgaagc cccactttga tgaccacaaa atttagcccc catacgacca
    23221 ctaccaggag cgccaacaca ccatcttttc gcgacattct accgcctctg aatttcatct
    23281 tttgtaagca atcaacttag ctgaatttac ttttctttaa cagttgattc gttagtcgcc
    23341 ggttacgacg gcattaatgc gcaaataagt cgctatactt cggatttttg ccatgctatt
    23401 tctttacatc tctaaaacaa aacataacga aacgcactgc cggacagaca aatgaactta
    23461 tccctacgac gctctaccag cgcccttctt gcctcgtcgt tgttattaac catcggacgc
    23521 ggcgctacgc tgccatttat gaccatttac ttgagtcgcc agtacagcct gagtgtcgat
    23581 ctaatcggtt atgcgatgac aattgcgctc actattggcg tcgtttttag cctcggtttt
    23641 ggtatcctgg cggataagtt cgacaagaaa cgctatatgt tactggcaat taccgccttc
    23701 gccagcggtt ttattgccat tcctttagtg aataacgtga cgctggtggt gctctttttt
    23761 gcccttatta actgcgccta ttccgttttt gctaccgtgc tgaaagcctg gtttgccgac
    23821 aatctttcgt ccaccagcaa aacgaaaatc ttctcaatca actacaccat gctcaacatt
    23881 ggctggacca tcggtccgcc gctcggcacg ctgttggtga tgcagagcat caatctgccc
    23941 ttttggctgg cagctatctg ttccgcgttt cccatgcttt tcattcaaat ttgggtaaag
    24001 cgcagcgaga aaatcatcgc cacggaaaca ggcagtgtct ggtcgccgaa agttttatta
    24061 caagataagg cactgttgtg gtttacctgc tctggctttc tggcttcttt tgttagcggt
    24121 gcctttgcct cgtgcatttc acaatatgtg atggtgattg ctgatgggga ttttgccgaa
    24181 aaggtggtcg cggttgttct tccggttaat gctgccatgg tagttacgtt gcaatattcc
    24241 gtgggccgcc gccttaaccc ggctaacatc cgcgcgctga tgacagcagg caccctctgt
    24301 ttcgtcatcg gtctggtcgg ttttattttt tccggcaaca gcctgctatt gtggggtatg
    24361 tcagctgcgg tatttactgt cggtgaaatc atttatgcgc cgggcgagta tatgttgatt
    24421 gaccatattg cgccgccagg aatgaaagcc agctattttt ccgcccggtc tttaggctgg
    24481 cttggtgccg cgattaaccc attagtgagt ggcgtagtgc taaccaacct gccgccttcc
    24541 tcgctgttta tcatcttagc gttggtgatt attgctgcgt gggtactgat gttaaaaggg
    24601 attcgtgcaa gaccgtggag gcagcccgcg ctttgttgat ttaagtcgaa cacaataaaa
    24661 atttaattca gccttcgttt aggttacctc tgctaatatg tttctcattg agatgaaaat
    24721 taaggtaagc gaggaaacac accacaccat aaacggaggc aaataatgct gggtaatatg
    24781 aatgttttta tggccgtact gggaataatt ttattttctg gttttctggc cgcgtatttc
    24841 agccacaaat gggatgacta atgaacggag ataatccctc acctaaccgg ccccttgtta
    24901 cagttgtgta caaggggcct gatttttatg acggcgaaaa aaaaccgcca gtaaaccggc
    24961 ggtgaatgct tgcatggata gatttgtgtt ttgcttttac gctaacaggc attttcctgc
    25021 actgataacg gatcgttgac acagtagcat cagttttctc aatgaatgtt aaacggagct
    25081 taaactcggt taatcacatt ttgttcgtca ataaacatgc agcgatttct tccggtttgc
    25141 ttaccctcat acattgcccg gtctgctctt ccaatgacca catccagagg ctcttcagga
    25201 aatgcgcgac tcacacctgc tgtcacggta atgttgatat gcccttcaga atgtgtgatg
    25261 gcatggttat cgactaactg gcaaattctg acacctgcac gacatgcttc ttcatcatta
    25321 gccgctttaa caataatgat aaattcttcg cccccgtagc gataaaccgt ttcgtaatca
    25381 cgcgtccaac tggctaagta agttgccagg gtgcgtaata ctacatcgcc gattaaatgc
    25441 ccgtaggtat cattaaccaa tttaaatcgg tcaatatcca agaacattaa ataaagattc
    25501 agaggatcag tgttgcgtaa ctgatgatca aaggattcat caagaacccg acgccccggc
    25561 aatcccgtca aaacatccat attgctacgg atcgtcagca aataaatttt gtaatcggtt
    25621 aatgccgcag taaaagaaag caacccctcc tgaaaggcgt cgaaatgcgc gtcctgccag
    25681 tgattttcaa caatagccag tattaattcc cgaccacagt tatgcatatg ttgatgggca
    25741 gaatccatta gccgaacgta aggtaattca tcgttatcga gtggccccag atgatcaatc
    25801 caccgaccaa actggcacag tccataagaa tggttatccg ttatttctgg cttactggca
    25861 tctctcgcga ccacgctgtg aaacatactc accagccact ggtagtgggc atcgatagcc
    25921 ttattgagat ttaacaagat ggcatcaatt tccgttgtct tcttgatcat tgccactcct
    25981 ttttcacagt tccttgtgcg cgctattcta acgagagaaa agcaaaatta cgtcaatatt
    26041 ttcatagaaa tccgaagtta tgagtcatct ctgagataac attgtgattt aaaacaaaat
    26101 cagcggataa aaaagtgttt aattctgtaa atcacctctg cattatcgta aataaaagga
    26161 tgacaaatag cataacccaa taccctaatg gcccagtagt tcaggccatc aggctaattt
    26221 atttttattt ctgcaaatga gtgacccgaa cgacggccgg cgcgcttttc ttatccagac
    26281 tgccgctaat gttgatcatc tggtccggct gaacttctcg tccatcaaag acggccgcag
    26341 gaataacgac attaatttca ccgctcttat cgcgaaaaac gtaacggtcc tctcctttgt
    26401 gagaaatcaa attaccgcgt agtgaaaccg aagcgccatc gtgcatgttt tttgcgaaat
    26461 caacggtcat tttttttgca tcatcggttc cgcgatagcc atcttctatt gcatgaggcg
    26521 gcggtggcgc tgcatcctgt tttaaaccgc cctggtcatc tgccaacgca taaggcatga
    26581 caagaaaact tgctaataca atggcctgaa atttcatact gactccttaa ttgcgtttgg
    26641 tttgacttat taagtctggt tgctattttt ataattgcca aataagaata ttgccaattg
    26701 ttataaggca tttaaaatca gccaactagc tgtcaaatat acagagaatt taactcacta
    26761 aagttaagaa gattgaaaag tcttaaacat attttcagaa taatcggatt tatatgtttg
    26821 aaaattatta tattggacga gcatacagaa aaagcaaatc acctttacat ataaaagcgt
    26881 ggacaaaaaa cagtgaacat taatagagat aaaattgtac aacttgtaga taccgatact
    26941 attgaaaacc tgacatccgc gttgagtcaa agacttatcg cggatcaatt acgcttaact
    27001 accgccgaat catgcaccgg cggtaagttg gctagcgccc tgtgtgcagc tgaagataca
    27061 cccaaatttt acggtgcagg ctttgttact ttcaccgatc aggcaaagat gaaaatcctc
    27121 agcgtaagcc agcaatctct tgaacgatat tctgcggtga gtgagaaagt ggcagcagaa
    27181 atggcaaccg gagccataga gcgtgcggat gctgatgtaa gtattgccat taccggctac
    27241 ggcggaccgg agggcggtga agatggtacg ccagcgggta ccgtctggtt cgcgtggcat
    27301 attaaaggcc agaactacac tgcggttatg cattttgctg gcgactgcga aacggtatta
    27361 gctttagcgg tgaggtttgc cctcgcccag ctgctgcaat tactgctata accaggctgg
    27421 cctggcgata tctcaggcca gccattggtg gtgtttatat gttcaagcca cgatgttgca
    27481 gcatcggcat aatctgagga gccttaccgc gccattgtcg atacaggtgt tccagatctt
    27541 tgctgttacc tctggaaagg atcgcctcgc gaaaacgcag cccattttca cgcgttaatc
    27601 cgccctgctc aacaaaccac tggtaaccat catcggccaa catttgcgtc cacagataag
    27661 cgtaataacc tgcagcatat ccgccaccaa aaatatgggc gaaataactg ctgcgatagc
    27721 gtggcggtat agcaggaaga tccatatttt ccgccaccag cgcccgcaat tcaaaatcat
    27781 cgacatcctg cattgcttcg ttttcttcca ggcaatgcca gcgcatatcg agaagtgcgg
    27841 cgctaagcag ttcgctcatc tcataccctt tgttgaacag gctggcatta cgcattttct
    27901 gttgcagttc gtcaggcatt gctgccccgc tctgataatg ccgggcgtag cgagcgaata
    27961 cctgcggatg cgttgcccag tgttcgttga tttgcgacgg aaattcgaca aaatcacgcg
    28021 gcgtgttggt gccggaaagc gtggcataac gctggcgggc aaaaaggccg tgcagcgtat
    28081 gaccaaattc atggaataag gttatgacat catcccagag taacaacgca ggctcaccgg
    28141 cagcgggttt ctgataattg caaacgttat aaattaccgg atgcgtttca ttaagcgttg
    28201 attgctcaac aaaattgccc atccatgcac cgccgctttt tgaatcacgg gcgaagaaat
    28261 caccgtaaaa taacgccagt cccacgccat tatgatcaaa aatttcccac acacgaacgt
    28321 caggatggta gacaggaata tcaaaacgtt cgacaaactt aataccgaag agctgattcg
    28381 cggtccagaa tacaccttca ttcaacaccg tgtttaattc aaaatatggc ttgagctgcg
    28441 cctcatcaag atcaaatttc tcccgccgta cctgttcggc ataaaatgcc cagtcccacg
    28501 gctgcgcgct aaacccgcct tgctgcttat cgataaccgc ctgtatggag gctaattcat
    28561 cgctagcacg ttgacgcgcc gctggaacaa tttcccgcat aaagttgagt gctgcttctg
    28621 gcgtttttgc catctgatcg gcgattttcc atgcggcata atgaggaaaa ccaagcagtt
    28681 tcgcctgctg tgcgcgaatc tctaccagac gttgaatgat agcgcgggta tcattggcat
    28741 catttttttc cgctcgcgtc cagcccgcaa taaacagttt ttcacgcgtc gcacgatcgc
    28801 gcatctcggc aagcgccggt tgttgggtgg tattcagcag cggaatcagc catttgttat
    28861 ccagaccttt ctcgcgagcc gcctctgccg ccagcgcaat ctcttgctca ctcattcctg
    28921 ccagctgcgc gatatcgttc acaaccagac cgccggattt atttgctgcc agtaatcgct
    28981 ggttaaactg gctggtcagg gtcgcagctt ctgtattcag tacttttaat tttgctttat
    29041 cagcttgcgc aagtttggct ccggcaagga caaaacgttg atgaatcacc tccaccaggc
    29101 ggatggattc actatcaagc cccagagatt cacggcgctg ccagacagca tctacccgcg
    29161 cgaataattc accgttcaga tagatatcat tagccagttc cgccagttca gcggaaaact
    29221 gctcgtcaag acgctgtaat tcatcattgg tatgcgccgc agtcatcgca aaaaagacac
    29281 tggtaacgcg ggtaagtaat tctccgcttt gttccagcgc cagaatagta ttgttgaaat
    29341 caggtgtttg cgggttaagc gcaatagcgg caatttctgc ccgtttttgc tgcattccct
    29401 catcgaatgc cgggcgatag tgatgattgg caatttgatc aaaatgggga gccagatacg
    29461 gcagtgtgct ttgcacaagg aaaggattca ttgttgtcat tttcttctcc tgaacgcgag
    29521 gtgttccata gcgtaggctt actgataacg gagtgcaatc ttgcaaacca gtattacccg
    29581 ctcttaagca tcacgtgcta tgttattgac acacaaaagc gttgaggaac agtgagatga
    29641 tcgttttagt aactggagca acggcaggtt ttggtgaatg cattactcgt cgttttattc
    29701 aacaagggca taaagttatc gccactggcc gtcgccagga gcggttacag gagttaaaag
    29761 acgaactggg agataatctg tatatcgccc aactggacgt tcgcaaccgc gccgctattg
    29821 aagagatgct ggcatcgctc cctgccgagt ggtgcaatat tgatatcctg gtaaataatg
    29881 ccggcctggc gttgggcatg gagcctgcgc ataaagccag cgttgaagac tgggaaacga
    29941 tgattgatac caacaacaaa ggcctggtat atatgacgcg cgccgtctta ccgggtatgg
    30001 ttgaacgtaa tcatggtcat attattaaca ttggctcaac ggcaggtagc tggccttatg
    30061 ccggtggtaa cgtttacggt gcgacgaaag cgtttgttcg tcagtttagc ctgaatctgc
    30121 gtacggacct gcatggtacg gcggtgcgcg tcaccgacat cgaaccgggt ctggtgggtg
    30181 gcaccgagtt ttccaatgtc cgctttaaag gcgatgacgg taaagcggaa aaaacctatc
    30241 aaaataccgt tgcattgacg ccagaagatg tcagcgaagc cgtttggtgg gtgtcaacgc
    30301 tgcctgctca cgtcaatatc aataccctgg aaatgatgcc ggttacccaa agctatgccg
    30361 gactgaatgt ccaccgtcag taatttttat acccggcgta actgccgggt tattgcttgt
    30421 cacaaaaaag tggtagactc atgcagttaa ctcactcaca agcaagaacg aatgaccgtc
    30481 gaaacgcaac ttaatcccac acagcctgtc aatcagcaaa tttatcgtat ccttcgtcgc
    30541 gacattgtcc attgcctgat tgctccaggc acaccgttgt cggaaaaaga agtttctgtt
    30601 cgtttcaatg tgtcacgcca gccggttcgt gaagccttta ttaaactggc ggaaaacggc
    30661 ctgattcaaa ttcgtccaca gcgtggcagc tacgtcaaca aaatttccat ggcccaggtg
    30721 cgcaacggca gttttatccg tcaggccatt gagtgcgcgg tggcgcgtcg ggcggcgagc
    30781 atgattaccg aaagccagtg ctaccaactg gaacaaaatc ttcaccagca acgcattgcc
    30841 attgagcgca agcaactgga tgattttttt gaacttgatg acaacttcca tcaactcctg
    30901 acgcagattg ccgactgtca actggcgtgg gataccattg agaacctgaa agcgaccgtt
    30961 gatcgcgtgc gctatatgag tttcgaccat gtttctccac cagaaatgct gttacgccag
    31021 catcttgata ttttctctgc cctgcaaaaa cgtgatggcg atgcggtaga acgtgcaatg
    31081 acgcaacatt tgcaggaaat cagcgaatcc gtgcgccaga tccgccagga aaacagcgac
    31141 tggtttagcg aagagtaatt catttactct catcccatcc gggatgagag gcttttcccc
    31201 ccgccttacg gctcatgcat gcatcaaaaa agatgtgagc ttgatcaaaa acaaaaaata
    31261 tttcactcga caggagtatt tatattgcgc ccgttacgtg ggcttcgact gtaaatcaga
    31321 aaggagaaaa cacctatgac gacctacgat cgtaaccgta acgcaatcac cactggcagc
    31381 cgtgttatgg ttagcggcac cggtcacact ggcaagatcc tgtcgattga tactgaaggt
    31441 ctgaccgctg agcaaatccg ccgcggaaaa accgtagttg ttgaaggttg tgaagagaaa
    31501 ctggcacctc tggacctgat tcgtctcggc atgaactaag tgtgtgaatg ccgccgatgg
    31561 cggcattgct ttttttactt cacggaatat ttcgccacgg tcgctttcgc gccatgcgct
    31621 aatcaagaca agtacgcttc cgtcactttt gcagtaaaca agctattgtc tggcaaatca
    31681 tcaccaaaga ttgccttaat cgccagcaat gactggacgc gcgctttccc ttcggcacta
    31741 ctttgtacag ccttctgaat aacaggtaac agtgggtcac tgatttctat cggatttccc
    31801 tgttcatcaa caccaccgac ataacgcatc caacccgcga cgcccagcgc cagcagatcg
    31861 aacttgctgt catgcgccag atgccagcga acagaatcca acatccgctg tggcaatttc
    31921 tggctaccat ccatcgcaat ctgccaggtt cgatgacgta acgccgggtt gctatagcgt
    31981 tcaattaatc ggttagcgta atcttgcaaa tcaacgccct gcactttcag cgtcggcgct
    32041 tgttcctgca acatcaagcc atacgccgca taacgataat gttcatcttc catgcagtca
    32101 ttaatgtgct gatatccagc aagataaccc aaatacgcca ggaatgaatg actgccgttg
    32161 agcatgcgca acttcatctc ttcataaggc agcacatcac taaccagttc ggctcccgct
    32221 ttttcccatt ctggacgtcc ggcaacaaag ttatcttcta ttacccactg gcggaaaggt
    32281 tcacaggcaa cgcccgcagg atcgcccaca ccggtaagtt gttcgatttt cgccagcgta
    32341 tcctctgtca ctgcgggcac aatacggtcc accattgttg atgggaaagt cacgttatct
    32401 tcgatccatt gtgccagttt tacatcaaca gctttcgcgt atgaagtgac aacgtcacgc
    32461 ataacatgac cgttttctgg catgttgtca catgacatga cggtaaatgc gggaagtcct
    32521 gccgctttac ggcgggccag cgcctcaaca atcacccctg ttgctgtttt cggctggtgg
    32581 ggattttgca cgtcggcagc gaccatcggg tgatcgagca ttaactgtcc ggtcgccgga
    32641 gagtggaaat accctttttc ggtgattgtc agagagacaa tcgcgatttg cggttcacac
    32701 atcgcagcca acacggtttc taagccatcc atctgtacgt gcaaggcttt tttaacgacg
    32761 ccaacgacgc gagccgtcca cgcatcggcc gacatttccg caacggtata aagattatct
    32821 tgctgttgta aatcggcaat ttgctgttcg ccgccgatta agttgacctc ataatatccc
    32881 cagtcactga aatgttctgt agcaagaata tcggcataca cgccctgatg cgcacggtga
    32941 aatgcaccaa agcctaaatg aataattctt ggggccaggt tattacgatc ataaacaggg
    33001 agtgtcgctt ttgctgataa caaattattt cccataacaa ttccttaaat ataaatatgg
    33061 caagctatat gttttgttat atgaataaaa atcccctctc cggtaagaga agggattgag
    33121 gatttacaga cttctggaag gttgcgcagc tcttacaaca cgcggttgat cttccgcagc
    33181 gtcttccagc gcacttaaat cacggtcttt cacctctggc attttcagcg cagagattaa
    33241 accaattact gaatatgcca tgatcataat ggcgatcgga taccaggatt ccgtcatggt
    33301 gcagaaaata cccgccagga taggaccaaa accggaagcg ataagaccac caatttcttt
    33361 agaaatagcc atccgggtaa agcggttttt acagccgaac atttctgcca tggtaatgtt
    33421 ttccagagca aataatccca gcaccgcaca gttatgaatc acaatcagtg caaccataat
    33481 ggtgctcggg gcatagcttt tatctacaat gatagaaagc attggccatg ccagcacaat
    33541 cgcggaggta ttcataataa tatacgggat ccggcgacca attttatcgg ataaccaacc
    33601 aaggaacgga atggtcataa agccgagaat cgaactaatc atcaatgcat ctgttggaat
    33661 tgctttgtta aacaataacg tctgcactaa atagcctgca aggaaagtct gaattaaccc
    33721 ggagttaccc gcctgaccaa aacgcagccc tgttgccagc cagaaggatt tgctctggaa
    33781 catgctacca gcaggtgcag gttttgctgt gggttggttg ctgtcattaa ccttctcaaa
    33841 gaccgggctt tctttcagat tcatacgtaa ccagatagca aagaccatca cgacaacgct
    33901 cgccaggaac ggtatacgcc atccccacgc cagcagttcc tctttactga gaatgaagaa
    33961 cataaaggcc cagattgccg ttgcgctcaa ggttccgcag ttagttccca tagctacaaa
    34021 tgaggagata attccgcgct taccttttgg tgcatattcc gccagcatcg taccggcacc
    34081 ggaaatttcc gcacctgcac ccaacccctg aataatacgc agcgtcacca gcaagatggg
    34141 ggtaaaaaca ccaatctgtg cataggtcgg taacacacca attaaggtgg tacagatccc
    34201 catcatggtg atggtaataa agagcacttt tttacgccct attctgtcgc ccattttgcc
    34261 gaaaataaat gctccgacaa tacgcgccac ataacctgca ccgtaggttc ccattgccaa
    34321 aattaacgcc attgccgttg atgattcagg aaaaaatatt tcatgaaaca ctaacgctgc
    34381 gccgagcgaa tataactgga aatccataat tcatagatgt tttttactat tctgtgggtt
    34441 tttgggtgtt ttctaagttt tttcagatgg ttgtattttt tctaaaaatc cctaatctcg
    34501 attttgctgt ttatttgagg cctttttatg tcccatatat gccccacaga taccccgcag
    34561 ccaaaatcaa caaaatgcca aaaggttctg ttcctgccct gcaacaagaa atgctgcgac
    34621 gtgtcagtaa acgttatgac gatgtagaag tgatcatcaa atccaccagc aacgatggcc
    34681 tttcagttac tcgcaccgcc gataaagatt ctgcaaaaac ttttgttcag gagacgctga
    34741 aagatacctg ggagtctgct gacgagtggt ttgttcgcta attaacgagt aaaatcagta
    34801 acagctggaa atcattcaat actcacacta tctaaaagtt caccagccaa cagcggtaag
    34861 ttctttcatc cgacgtgccg cggatcctat tattctagta tgaaatccct aaccgatttc
    34921 aaattctcca gactgctcag attctctttt atcccacgct ggcgctgata aatctcgtca
    34981 ttgcaatcga cctgcgctat cgctgccgca gttcttccag tttctgcatc gataactcca
    35041 tcttctgagc aataaacctc acctatgcaa tattttatgt caattatttc aattgcatta
    35101 atatcttgaa tggattacat agagttaatg tatccagtac tccctattct cttgcatata
    35161 atacatgttg caacttacat ctcagcgcta tgaaaaaaca ccacctctct ctttatgaaa
    35221 ttctggattt accaagcgct aatttatcat ttcaatctac ctttaagcat tgcatttatc
    35281 tccctacgag gtcatacttt aggaagttaa atatgaatga taatatacct acagcgcgaa
    35341 atcacaaaca atcgacttgt attacagaaa aaaacatgcc tatcttttta aacttcacag
    35401 caggcagtat cttacctgag aatgagctag catctttacg ttatattgtg cagcaaaatc
    35461 aaaatgatac tgtaatcata aaagaacgtt ataaaatgga tatccgttat atcgaatcag
    35521 tcaatggttt tacagtaaat cctgtatgca gtaatcattt ctccatattt atggcgagac
    35581 aaaacactat tgctcgcaac ctggaacagc agatcaacaa cggacgaagt tttgcacaaa
    35641 tatctcagga ttttatgctt caattatctt caaatatagg atggaaaaaa ggggccgaaa
    35701 acgcccttaa aaataaaatc cattctcatt catttgttgt aaatcctgat gaattctctt
    35761 gcgacacaca atttcttaag tgcccaataa cattatgcgt tccagaaaaa ggggtttttg
    35821 tcaagaacgc actgaactcc aacatatgca ctctttatga taagtctgcg ttcatgaatc
    35881 tcacaagaga acatctaccc caccctctca gcagggaaaa gatagtaaaa gaaatgatta
    35941 ttgaaaggaa tatgtgttat tttgacacca taagtcagca tttcataatt atggatgcag
    36001 accaacagaa acagcattgt aaataaaatg taataattac atactattag tgattctcat
    36061 gcatcgtaag cggctcgcca gaaccgtatt gatatttact gagcgctcag atcaactttc
    36121 catggcaaca gatcgcgtac ccggtttgcc ggccagtcct ggatatgctc ccgccgacaa
    36181 tattaatggc caggaaatgt ttaaaacaat gattctgttt cctaaccatc aagtgtatat
    36241 gctggctcaa ttactcaata tctatgggtt gagttctacc caaagtggct ctgtagtcgc
    36301 ttcttcttac agtatgttca ggtgttgcag gcacagttgt ggagcgtaga tgcgttgttg
    36361 gtttaccatg ctctggtaca taaaaaacgc cagtgccatg gtaacctgat atagaaacgt
    36421 taattccgcg acgttcaaac tcggcataga cgtgttctgc taaagacctc ttttgaccaa
    36481 ataatgccct ggccaaccag ccgttattca tatttgcgga tttttctatt tcatcaggag
    36541 agaagtcttt gttttttatt atgttggctg agttacagga tgttaatctg atatcatcta
    36601 tttcatgaag attatgttga acaatgcggt cgacaatatc tgacggtgat aaaaattcat
    36661 ctccgcactt aagaagaggt agaccagcag aaccatgccc agacagataa attttgttgt
    36721 attttcctgg tgttaaatca tctggcagga tggttttcat ctctgtcgct gtaatactta
    36781 tagctgcagc agcaacaaca tcgctattac ttgattgtag atggctttta tttgctccag
    36841 gatatgtaaa ctccattttc cttttatcaa aatcttgttt tgtagcatca ttcaggaata
    36901 ataaatcata cggttttttc cccgttgtga ctcttgaaac attttggcca agaatattaa
    36961 gagtatagtc attaacggat ttcattccta aaaaaacaag taatccttct ttctcttgcg
    37021 ctttttcgat tgttctccct gctaaagtta ttgggatgga ttgagattgt tttgtcggaa
    37081 ctgatacgct ggcgctaata ggtagtaggg ttcttatgct aaacatacaa cctctttctt
    37141 aattttcgaa cctgtttagg attctgtgta aattcaaaat aaacctgtct gaacattcct
    37201 aacaaacatc cacc
//
LOCUS       DAZKHC020000040        36851 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZKHC020000040 DAZKHC020000000
VERSION     DAZKHC020000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 36851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.040"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..842
                     /gene="ygcG"
                     /locus_tag="ACHOSM_RS11570"
                     /old_locus_tag="pgaptmp_002314"
     CDS             <1..842
                     /gene="ygcG"
                     /locus_tag="ACHOSM_RS11570"
                     /old_locus_tag="pgaptmp_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU4558825.1"
                     /translation="CSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTHKLESIFNNT
                     QVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSDRTVRIQVGYGL
                     EEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQYPTNPSESESAS
                     SSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDLFLFSDKIFSIA
                     VFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSGGGGSSGGGGAS
                     GRW"
     gene            complement(902..2200)
                     /gene="eno"
                     /locus_tag="ACHOSM_RS11575"
                     /old_locus_tag="pgaptmp_002315"
     CDS             complement(902..2200)
                     /gene="eno"
                     /locus_tag="ACHOSM_RS11575"
                     /old_locus_tag="pgaptmp_002315"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HGU4558826.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIALALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2288..3925)
                     /gene="pyrG"
                     /locus_tag="ACHOSM_RS11580"
                     /old_locus_tag="pgaptmp_002316"
     CDS             complement(2288..3925)
                     /gene="pyrG"
                     /locus_tag="ACHOSM_RS11580"
                     /old_locus_tag="pgaptmp_002316"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HGU4558827.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4153..4944)
                     /gene="mazG"
                     /locus_tag="ACHOSM_RS11585"
                     /old_locus_tag="pgaptmp_002317"
     CDS             complement(4153..4944)
                     /gene="mazG"
                     /locus_tag="ACHOSM_RS11585"
                     /old_locus_tag="pgaptmp_002317"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HGU4558828.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5015..5350)
                     /gene="mazF"
                     /locus_tag="ACHOSM_RS11590"
                     /old_locus_tag="pgaptmp_002318"
     CDS             complement(5015..5350)
                     /gene="mazF"
                     /locus_tag="ACHOSM_RS11590"
                     /old_locus_tag="pgaptmp_002318"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HGU4558829.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5350..5598)
                     /gene="mazE"
                     /locus_tag="ACHOSM_RS11595"
                     /old_locus_tag="pgaptmp_002319"
     CDS             complement(5350..5598)
                     /gene="mazE"
                     /locus_tag="ACHOSM_RS11595"
                     /old_locus_tag="pgaptmp_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HGU4558830.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5676..7910)
                     /gene="relA"
                     /locus_tag="ACHOSM_RS11600"
                     /old_locus_tag="pgaptmp_002320"
     CDS             complement(5676..7910)
                     /gene="relA"
                     /locus_tag="ACHOSM_RS11600"
                     /old_locus_tag="pgaptmp_002320"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HGU4558831.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7958..9259)
                     /gene="rlmD"
                     /locus_tag="ACHOSM_RS11605"
                     /old_locus_tag="pgaptmp_002321"
     CDS             complement(7958..9259)
                     /gene="rlmD"
                     /locus_tag="ACHOSM_RS11605"
                     /old_locus_tag="pgaptmp_002321"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGU4558832.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9316..12072
                     /gene="barA"
                     /locus_tag="ACHOSM_RS11610"
                     /old_locus_tag="pgaptmp_002322"
     CDS             9316..12072
                     /gene="barA"
                     /locus_tag="ACHOSM_RS11610"
                     /old_locus_tag="pgaptmp_002322"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HGU4558833.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPSSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12304..13644)
                     /gene="gudD"
                     /locus_tag="ACHOSM_RS11615"
                     /old_locus_tag="pgaptmp_002323"
     CDS             complement(12304..13644)
                     /gene="gudD"
                     /locus_tag="ACHOSM_RS11615"
                     /old_locus_tag="pgaptmp_002323"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HGU4558834.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13665..15005)
                     /gene="gudX"
                     /locus_tag="ACHOSM_RS11620"
                     /old_locus_tag="pgaptmp_002324"
     CDS             complement(13665..15005)
                     /gene="gudX"
                     /locus_tag="ACHOSM_RS11620"
                     /old_locus_tag="pgaptmp_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HGU4558835.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPSEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15007..16359)
                     /gene="gudP"
                     /locus_tag="ACHOSM_RS11625"
                     /old_locus_tag="pgaptmp_002325"
     CDS             complement(15007..16359)
                     /gene="gudP"
                     /locus_tag="ACHOSM_RS11625"
                     /old_locus_tag="pgaptmp_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HGU4558836.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16794..17243)
                     /gene="yqcA"
                     /locus_tag="ACHOSM_RS11630"
                     /old_locus_tag="pgaptmp_002326"
     CDS             complement(16794..17243)
                     /gene="yqcA"
                     /locus_tag="ACHOSM_RS11630"
                     /old_locus_tag="pgaptmp_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGU4558837.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17261..18043)
                     /gene="truC"
                     /locus_tag="ACHOSM_RS11635"
                     /old_locus_tag="pgaptmp_002327"
     CDS             complement(17261..18043)
                     /gene="truC"
                     /locus_tag="ACHOSM_RS11635"
                     /old_locus_tag="pgaptmp_002327"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HGU4558838.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18043..18372)
                     /gene="yqcC"
                     /locus_tag="ACHOSM_RS11640"
                     /old_locus_tag="pgaptmp_002328"
     CDS             complement(18043..18372)
                     /gene="yqcC"
                     /locus_tag="ACHOSM_RS11640"
                     /old_locus_tag="pgaptmp_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HGU4558839.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18994..19539)
                     /gene="syd"
                     /locus_tag="ACHOSM_RS11645"
                     /old_locus_tag="pgaptmp_002329"
     CDS             complement(18994..19539)
                     /gene="syd"
                     /locus_tag="ACHOSM_RS11645"
                     /old_locus_tag="pgaptmp_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HGU4558840.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19607..20455
                     /gene="queF"
                     /locus_tag="ACHOSM_RS11650"
                     /old_locus_tag="pgaptmp_002330"
     CDS             19607..20455
                     /gene="queF"
                     /locus_tag="ACHOSM_RS11650"
                     /old_locus_tag="pgaptmp_002330"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HGU4558841.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSLQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20567..21931
                     /gene="ppnN"
                     /locus_tag="ACHOSM_RS11655"
                     /old_locus_tag="pgaptmp_002331"
     CDS             20567..21931
                     /gene="ppnN"
                     /locus_tag="ACHOSM_RS11655"
                     /old_locus_tag="pgaptmp_002331"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HGU4558842.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            22488..23777
                     /gene="sdaC"
                     /locus_tag="ACHOSM_RS11660"
                     /old_locus_tag="pgaptmp_002332"
     CDS             22488..23777
                     /gene="sdaC"
                     /locus_tag="ACHOSM_RS11660"
                     /old_locus_tag="pgaptmp_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HGU4558843.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            23835..25202
                     /gene="sdaB"
                     /locus_tag="ACHOSM_RS11665"
                     /old_locus_tag="pgaptmp_002333"
     CDS             23835..25202
                     /gene="sdaB"
                     /locus_tag="ACHOSM_RS11665"
                     /old_locus_tag="pgaptmp_002333"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HGU4558844.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDAVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            25314..26069
                     /gene="xni"
                     /locus_tag="ACHOSM_RS11670"
                     /old_locus_tag="pgaptmp_002334"
     CDS             25314..26069
                     /gene="xni"
                     /locus_tag="ACHOSM_RS11670"
                     /old_locus_tag="pgaptmp_002334"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HGU4558845.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     EPHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(26124..27272)
                     /gene="fucO"
                     /locus_tag="ACHOSM_RS11675"
                     /old_locus_tag="pgaptmp_002335"
     CDS             complement(26124..27272)
                     /gene="fucO"
                     /locus_tag="ACHOSM_RS11675"
                     /old_locus_tag="pgaptmp_002335"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HGU4558846.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVHSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSQEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(27300..27947)
                     /gene="fucA"
                     /locus_tag="ACHOSM_RS11680"
                     /old_locus_tag="pgaptmp_002336"
     CDS             complement(27300..27947)
                     /gene="fucA"
                     /locus_tag="ACHOSM_RS11680"
                     /old_locus_tag="pgaptmp_002336"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGU4558847.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            28494..29810
                     /gene="fucP"
                     /locus_tag="ACHOSM_RS11685"
                     /old_locus_tag="pgaptmp_002337"
     CDS             28494..29810
                     /gene="fucP"
                     /locus_tag="ACHOSM_RS11685"
                     /old_locus_tag="pgaptmp_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HGU4558848.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            29843..31618
                     /gene="fucI"
                     /locus_tag="ACHOSM_RS11690"
                     /old_locus_tag="pgaptmp_002338"
     CDS             29843..31618
                     /gene="fucI"
                     /locus_tag="ACHOSM_RS11690"
                     /old_locus_tag="pgaptmp_002338"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGU4558849.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            31727..33145
                     /gene="fucK"
                     /locus_tag="ACHOSM_RS11695"
                     /old_locus_tag="pgaptmp_002339"
     CDS             31727..33145
                     /gene="fucK"
                     /locus_tag="ACHOSM_RS11695"
                     /old_locus_tag="pgaptmp_002339"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HGU4558850.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQILEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            33147..33569
                     /gene="fucU"
                     /locus_tag="ACHOSM_RS11700"
                     /old_locus_tag="pgaptmp_002340"
     CDS             33147..33569
                     /gene="fucU"
                     /locus_tag="ACHOSM_RS11700"
                     /old_locus_tag="pgaptmp_002340"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HGU4558851.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            33627..34358
                     /gene="fucR"
                     /locus_tag="ACHOSM_RS11705"
                     /old_locus_tag="pgaptmp_002341"
     CDS             33627..34358
                     /gene="fucR"
                     /locus_tag="ACHOSM_RS11705"
                     /old_locus_tag="pgaptmp_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HGU4558852.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(34402..35502)
                     /gene="rlmM"
                     /locus_tag="ACHOSM_RS11710"
                     /old_locus_tag="pgaptmp_002342"
     CDS             complement(34402..35502)
                     /gene="rlmM"
                     /locus_tag="ACHOSM_RS11710"
                     /old_locus_tag="pgaptmp_002342"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HGU4558853.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(35495..35890)
                     /gene="ygdD"
                     /locus_tag="ACHOSM_RS11715"
                     /old_locus_tag="pgaptmp_002343"
     CDS             complement(35495..35890)
                     /gene="ygdD"
                     /locus_tag="ACHOSM_RS11715"
                     /old_locus_tag="pgaptmp_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HGU4558854.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35909..36826)
                     /gene="gcvA"
                     /locus_tag="ACHOSM_RS11720"
                     /old_locus_tag="pgaptmp_002344"
     CDS             complement(35909..36826)
                     /gene="gcvA"
                     /locus_tag="ACHOSM_RS11720"
                     /old_locus_tag="pgaptmp_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HGU4558855.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
BASE COUNT         9038 a         9759 c         8863 g         9191 t
ORIGIN      
        1 tatgttcctt tgttgcagcc caaccaacta ttgtcccaca attacagcaa caagttacgg
       61 atcttacgag tagcttaaac tcacaagaaa agaaagaact gacgcacaag ttagaatcta
      121 ttttcaataa tacccaagtg caaattgctg tattaattgt tcccacaacc aaagacgaga
      181 caattgaaca atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta
      241 atgatgggat actgatcatt gttgcctggt cagatcgtac tgtccgcatc caggtaggtt
      301 atgggctgga agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga
      361 tacccgcatt taaacaacaa aaatttgctc agggattaga gctagctata aacgctttga
      421 ataaccaact cacttcacaa caccaatatc cgactaatcc ttcagaaagt gaatcagcgt
      481 cttccagtga tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt
      541 tctggttttt tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt
      601 cagcaatcta tcttttagat ttattcctgt tctccgataa aattttttcc attgctgtat
      661 tttccttctt ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga
      721 aaagagcatc aggtaaaagt tatcattcag acaacagcgg ttccggtgga ggctcaagtg
      781 gcggcggatt tagcggcggt ggcggttctt ctggaggtgg cggcgcatcc ggccgctggt
      841 aactcaacca taaaaaatgc cagcccggag gctggcattt ttaaatcaga taaagtcagt
      901 cttatgcctg gcctttgatc tctttacgac cgttgtacgg tgctttttcg cccagagctt
      961 cttcgatacg aatcagctgg ttgtatttag caacacggtc agaacggctc atagaaccag
     1021 ttttgatctg gcctgcagca gtaccaacag ccaggtcagc gatggtagcg tcttcagttt
     1081 cgccagaacg gtgagagata actgcagtgt agccagcatc tttcgccatc ttgattgcag
     1141 ccagagtttc ggtcagagaa ccgatctggt tgaatttgat caggatggag ttagcgatac
     1201 ctttttcgat accttctttc aggatcttgg tgttggttac gaacaggtcg tcaccaacca
     1261 gctggatttt gtcgcccaga actttggtct ggtatgcgaa accgtcccag tcagattcgt
     1321 ccagaccgtc ttcgatagaa acgatcgggt actgtttggt cagttcttcc aggaagtgag
     1381 tgaattcttc agaggtgaac gctttgttgc cttcgccagc cagaacgtat ttaccatctt
     1441 tgtagaattc agaagctgcg cagtccatcg ccagagtgat gtctttgccc agttcataac
     1501 cagcagcttt aacagcttca gcgataacag ccagagcttc agcgttggaa cccaggttcg
     1561 gcgcatagcc accttcgtca ccaacagcag tgttcatgcc tttcgctttc agaacttttg
     1621 ccaggtgatg gaaaacttca gaacccatgc ggatggcttc tttcacagtt ttcgcgccaa
     1681 ccggctgaat catgaattcc tggatatcaa cgttgttgtc agcgtgctca ccaccgttga
     1741 tgatgttcat catcggaacc ggcatagagt atttgcccgg agtaccgttc agttcagcga
     1801 tgtgctcgta cagcggcata cctttagcag ctgcagcagc tttggcgtta gccagagata
     1861 cagccaggat tgcgttcgcg ccgaatttgg atttgttttc ggtgccgtcc aggtcgatca
     1921 tgatcttgtc aatgccagcc tgatctttag catctttgcc aatcagcgcc agagcgatcg
     1981 ggccgtttac cgcagcaaca gctttggtta cgcctttacc caggaaacgg gatttgtcgc
     2041 catcgcgcag ttccagagct tcacgggaac cagtagaagc acctgacgga gcagctgcca
     2101 taccgacgaa accaccctcc agatgtactt cggcttcaac agtcgggtta ccacgggagt
     2161 cgatgatttc acgaccgatg atttttacga ttttggacat taggttttcc tcaagtcact
     2221 agttaaactg aaactccaga caaacaacgc gtacccaggg tacgcgttgc cgttctaact
     2281 tttttactta cttcgcctga cgtttctgga actcgctggc ggctttcaca aagcctgcaa
     2341 acagcgggtg accatcacgt ggagtagaag taaactccgg atggaactgg caagccacga
     2401 accacgggtg attcggaact tcgatgatct cgaccaactg atcatccccg gaacggcccg
     2461 caacgcgcag acctgcatct tcaatctgtt tcaacagcat gttgttgact tcgtaacggt
     2521 gacgatgacg ctcaacaatt gtcggcgcat tgtacagctg gcgaaccagg ctatcgtcaa
     2581 ccaactggca ctgctgtgcg ccgagacgca tggtaccgcc gagatcgctc ttctcgctac
     2641 gaacttcaac gttgccgttt tcatcgcgcc actcggtaat cagcgccaca accgggtact
     2701 tacagtctgg cacaaattcc gtagagttgg cgttctccat gttggcaaca tggcgagcgt
     2761 aatcaattaa cgccacctgc atacccaggc aaatgcccag ataaggaata ttgttctcac
     2821 gcgcaaaacg cgcggtcgta atcatgcctt ctacgccacg atagccgaaa ccgccaggta
     2881 cgaggattgc gtccagacct ttaaggattt caacgccgcg cgtttcaaca tcttgtgaat
     2941 cgatcagttt gatgttgacg ctgacacgat tcttcagccc accgtgtttc agtgcttcga
     3001 tcactgattt ataagcatcc ggcagttcaa tgtacttgcc gaccataccg atggtgactt
     3061 cacttaccgg gttcgcttct tcgaagataa cctgttccca ttcggacaga ttcgcttccg
     3121 ggcagtttaa gctgaatcgt ttacaaatat aatcgtccag cccctgagat ttcaacaggc
     3181 ccggaatttt atagatggaa tcgacgtctt tcagagaaat aaccgctttt tccggaacat
     3241 tacagaacaa tgcaatcttc gcacgttcgt tcgccggaac agcgcgatct gaacgacaaa
     3301 tcaggatgtc aggctggata ccgatggaga gcagctcttt tacagagtgc tgagtcggtt
     3361 tggttttgac ttcaccagac gctgccatgt acggcaccag cgtcaggtgc ataaacagag
     3421 tgtgctcacg gccaatttca acagccatct ggcgaatcgc ttcgaggaac ggcaaggatt
     3481 cgatatcacc tactgtaccg ccgatttcta ccagtactac gtcatgacct tcgccacctt
     3541 ccagcacgcg ctctttgatt gcgttagtga tgtgcggaat aacctgcacg gttgcgccga
     3601 ggtagtcacc gcggcgttct ttacgcagaa cgtcagagta gatacgaccc gtggtgaagt
     3661 tgttgcggcg gctcattttg gtacgaatga aacgctcgta gtgccccagg tccaggtcgg
     3721 tttcagcgcc gtcttcagta acgaacactt ccccgtgttg gattgggctc atagtacctg
     3781 gatcgacgtt gatgtacgga tccagtttca tgatggtcac attgaggcca cgggcttcaa
     3841 gaatggctgc gagggaggct gcggcaatgc ctttacccag agaggatacg accccgccgg
     3901 tcacaaaaat atagttcgtt gtcatgctga acctgagaag ttaggttgaa aagacgatgg
     3961 aataaccagg acgggaaagc agtatacccg aacatgacct gtgccacaaa ctttcattat
     4021 ccctcctctt cgccagcaca ctattgaaat caggagtgag aaaatagccc ctttggggta
     4081 aatgtttttg acgcaaatca agcgcttgtc atttaaaaaa tgacacaaat ggcgcttgac
     4141 cgcgtaattc ccttagagat caatttcctg ccgttttacc tgttgccaga cttcttccat
     4201 tgtttcgagg tcaacacctg tcatttccag tccacgcgcg gcaacaatac gctccacttc
     4261 gcgaaaacga cgctcaaatt tttcgttcgc tttttgcaat gcgatttccg cttttgtccc
     4321 taaatgacga gccagattga ccgtggcaaa cagcaggtcc cccatttcct cctccagttt
     4381 agcctggtcg acaacagcct gccgcgcttc gtacatcacc tcgtcgatct cttcgtagac
     4441 tttatcgact accggaccaa gcgtcgtcca atcgaagcca acgttggcgc aacgtttctg
     4501 gattttttgc gcacgcatta aagccggtaa actacgagga atatcgtcca gcgccgaatg
     4561 ctgcgctttc tgcgcgcgct cttcggtttt gatttgctcc caacgggcaa gcacttcact
     4621 actgttttcg gcagaactat cagcaaaaac atgcggatgg cgacgctcta atttatcgct
     4681 aatagcagcg caaatatcat taaagtcaaa gcgcccttct tcctgagcca tttgcgcgta
     4741 aaacaccacc tggaatagca gatcgcccag ttcaccgcga agatcgtcaa aatcttcacg
     4801 ggcgatggcg tccagcactt cgtaggtttc ttcaagggtg taaggcgcaa tggtggcaaa
     4861 tgtctgctct ttatcccacg ggcagccgtt ttccggatcg cgcaggcgct gcataatagt
     4921 gagcaaacgg tcgatttgat tcattgaatt gtcctgaaaa ttgcgggtct gtcaggtgga
     4981 aacctgtgac cagaatagaa gtgagttagt aacactaccc aatcagtacg ttaattttgg
     5041 ctttaatgag ttgtaattcc tctggggcaa ctgttccttt cttcgttgct cctcttgccc
     5101 gccaggcgat actttttacc tgatcagcta acgctacgcc atcacgttcc tgaccggata
     5161 aaacaacttc gaacggatat ccttttgatt gcgttgtaca aggaacacac agacacatac
     5221 ctgttttgtt gttgtacatg aaaggactca ggacaacagc tggacgatgt ccagcttgct
     5281 cgctaccttt tgtcgggtca aaatcaaccc aaatcagatc gcccatatcg ggtacgtatc
     5341 ggcttaccat taccagactt ccttatcttt cggctctccc cagtcgatat tctcgtggag
     5401 gttttccggc gtgatgtcgt tgaccagttc agcaagcgta aatacgggct ctttacgcac
     5461 tggctcaata attaatttgc catccaccag gtcaatcttc acttcatcat caatattcag
     5521 attgagcgcc tgcattaacg tagccgggat ccgcaccgcc ggtgaatttc cccaacgctt
     5581 tacgctactg tggatcataa ccctttcctc aaaccgctat catatgtaga tacagtatat
     5641 atcaatctac attgtagata cgagcaaatt tcagcctaac tcccgtgcaa ccgacgcgcg
     5701 tcgataacat ccggcacctg gttgagttta cccagcacgc ggcccagcac ttgcaggttg
     5761 taaatctcaa tggtcatgtc gatggtcgcc agttgctgtt tggtgtcgct acggctggca
     5821 acgccaagta cgttcacctt ctcgttggcg agaatggtcg tgatatcacg taacaaccca
     5881 ctacgatcat tagccaccac gcggaccacc agcgaatatc cggcggagta gctctcgccc
     5941 cataccgcgt caacaatgcg ttctggcgca tgggagcgca gttccgccag ttgttcgcaa
     6001 tcggcgcggt gtactgaaat accgcgcccc tgggtaatga agccgacaat ctcatctcca
     6061 ggaatcggct ggcagcagcg cgcgatgtgg tgcatcaggt taccgacacc ttcaaccacc
     6121 acacgaccgt tatctttact gcggttttgc ggcgtgtagc ttttttgctg aagttgcttc
     6181 agcgcggcgg cgtcctgctc ttcggcactc ggcttattaa attgcgattg caggaagttc
     6241 accatctgat tgagacggat atccccgcca ccaatcgccg ccagcaactc gtcgacatca
     6301 ttgaagttgt aacgcggcag cagatgtttt tctgcttctt tcaggctgat ccccagatgt
     6361 tccagctcgt cgtcaaggat ttgccgccca gccagaatgt ttttgtcacg gtcctgttta
     6421 cggaaccagg cgtgaatttt cgaacgccca cggctggttg tgacgtaacc gaggtttggg
     6481 tttaaccagt cacggctggg gttcggctgt ttctgggtga taatttcaat ctggtcgccc
     6541 atctgcagct ggtaggtgaa cggcacaatg cgcccgccaa tttttgcccc gatgcagcgg
     6601 tgcccgacat cactgtggat gtggtaagcg aagtccagcg gcgttgatcc cgcaggcaaa
     6661 tcaacgacgt cacctttcgg cgtaaagacg tacacccggt cgtcaaagac ctggctgcgt
     6721 acttcgtcga gcatttcgcc ggaatcagcc atctcttcct gccacgcaat cagtttacgc
     6781 agccaggcaa ttcggtcttc atgtcccgaa cgtgcgccgc cagctgccgc gccttcttta
     6841 tatttccagt gcgcagcaac acccaactct gcatcttcat gcatctgttt ggtgcggatt
     6901 tggatctcaa cggttttgcc acccggcccc agaaccacgg tatgaataga ctgataaccg
     6961 tttggtttcg ggttagcgac gtaatcgtca aactcatccg gcaggtggcg atagtgagtg
     7021 tgcactatcc ccagtgcggc ataacaatcc tgtaaacgct cggcgacaat acgtaccgca
     7081 cgcacatcaa acagctcatc gaaggcgagg ttctttttct gcattttgcg ccagatgctg
     7141 tagatgtgtt tcggtcgacc atacacttca gctttaacgc cttcagcttt catctcagcg
     7201 cgcagatggc cgacaaactc ttcgatatag tgttcgcggt cgagacggcg ttcatgcagc
     7261 agttttgcga tgcgtttgta ttcggtcggg tggagataac ggaagcagta atcttccagc
     7321 tcccatttca gttgtccaat cccaagacgg ttcgccaacg gcgcgtagat attggtgcac
     7381 tcttttgccg ccagtacgcg ttcatcttcc ggcgcgtctt ttacttcgcg cagatgagca
     7441 atacgctctg ccagtttgat gaccacgcag cggaaatcat cgaccatcgc caataacatc
     7501 cggcgaacgt tatcgacctg ttcggaggaa acagaatcgg tatgcgtcgc tttcagctgg
     7561 cggatcgccg ccatatcacg cacgccgtga ataaggttaa cgaccgactt accgacgctc
     7621 tcacgcagca catcttcgct aactacgttg gcatccgcca gagggaaaag cagcgccgcc
     7681 cgcagcgtgt caatgtccat acttaatgtc gagaggatct ccaccatctc aacaccacgc
     7741 cacaataaca gactggcatc cggatgcccc tgcgtctgtt gcagacaata cgcccaggtt
     7801 tcggctaagc actcacacga cttctggctg gtaataccca gacttgcgat ccatttttcc
     7861 ggatcaaatt caccagcctt attgatatgt gcacttctta ccgcaaccat cgtcctctcc
     7921 tttagggacc agacctgccg aaattggcaa atcgtaacta tttaacgcgc gagaaaagta
     7981 ccatcgattc cagatgtccc gtgtgtggga acatatccag catcgccagt cgcgcaatgg
     8041 tatatcctgc ttttaataac gcttcgctat cccgagccag cgttgcagga ttacaggata
     8101 cataaactat acgaataggt tccagtttta taatttgctg cataacacct gcggcacctg
     8161 ctcgcgcggg gtccagcaac actttatcga agccgttttt cgcccacggc tgctttgtga
     8221 catcttcttc aagattttca tgataaaacg tcacattctg taagccatta agacgcgcat
     8281 tctgctggcc tttttccacc agcgccggaa caccttctac acccaccaca ctggcagctt
     8341 gtgtcgccaa tggcagtgta aagttgccca taccgcagaa cagatccagt acgcgatctt
     8401 caggttgcac atccagccat tccaacgcac gcgctaccat tttttggttc acacccgcat
     8461 tgacctgaat aaaatcgcgc gggctaaaag ttaagcgcaa cccgtttgag tcataccagg
     8521 gcatctcacc agagacggtt tcgagtatct cactatcggg ggcgagatac agatccaggc
     8581 cttcagaatg cgaaaagcgt tccagttttt cgcgatcagc cgaacttagc ggtgcggtat
     8641 ggcgcaaaat catcagcgtg ccgctggttg cctgtaccag ttcaacatga ccaagatggc
     8701 gtatagcttg taagctgccc aggcatgccc tgactttggg cagcaatgct tcaagttggg
     8761 gcactaaaat ggggcattgt ttaacgtcga caatgtcact ggagcccgct ttgcgaaagc
     8821 ccatctgaag ttgctgtgtt ttcggtaagt agttcaaact taaacgcgcg cgacggcgat
     8881 agccccaggg aacatcggcg atcacttcag agacttcgtg tttcattaat cgggcgagtg
     8941 ccgcactttt gcttcgctgc tgtaaatcca cgctggcgtg ttgttgctga cagccaccgc
     9001 atacgccaaa atgagggcag cgtggcgttt cgcgttccgg gctatcgctc aaccggcgca
     9061 cgactttagc gcgggcgtac tgttttttat cttcagtaac agtaacttcc gcgttttcct
     9121 gtggcagtaa tccaggaata aataacgctt tgccgttatg tcgcgccacg ccctgaccaa
     9181 aagagtcgag gtcgttgact gaaacggtta tgatctgacg cgtcgtcgtg cgtcgttttg
     9241 cagagtagaa ttgcgccatt ggcgagactt tctcaattta acagtgtgac cttaattgtc
     9301 ccataacgga actccatgac caactacagc ctgcgcgcac gcatgatgat tctgatcctg
     9361 gcaccgaccg tccttattgg tttattgctg agtatctttt tcgtcgtgca tcgctataac
     9421 gacttgcagc gtcaactgga agatgccggt gccagcatta ttgagccgct tgcagtttct
     9481 actgaatatg gcatgagcct gcaaaatcgc gaatctatcg gtcagttaat aagcgtactg
     9541 catcgtcgcc attccgatat tgttcgcgcg atttcggttt atgatgaaaa taaccgactc
     9601 tttgtcacct ccaattttca tcttgatccc tcatcaatgc agctcggcag caacgtgccg
     9661 tttcctcgcc agctcactgt cactcgtgac ggcgatatta tgatcctccg cacgccgatt
     9721 atttctgaga gttactcccc cgacgaatcg cccagtagcg atgccaaaaa tagtcaaaat
     9781 atgttgggat atattgcgct tgagctggat cttaaatcgg ttcgcttgca gcaatataaa
     9841 gagatcttta tttccagcgt gatgatgctg ttttgtatcg gtattgcgct tatttttggc
     9901 tggcgcttaa tgcgcgatgt aaccggtccg attcgcaaca tggtgaatac cgtcgaccgc
     9961 atccgtcgcg ggcaactcga cagccgggtg gaaggattta tgctcggcga gctggatatg
    10021 ctgaaaaacg gtatcaactc gatggcaatg tcgctggctg cttatcacga agagatgcag
    10081 cacaatatcg accaggcgac gtccgatctg cgtgaaacgc tggagcagat ggaaattcag
    10141 aacgttgagt tggatctggc gaaaaagcgc gcccaggaag cggcgcgtat taaatccgag
    10201 tttctggcaa atatgtcaca cgagctgcgt acaccactga atggtgttat tggctttacc
    10261 cgcctgacgc tgaaaacaga attaacacca acgcagcgcg atcacctgaa tacgattgaa
    10321 cgttcggcaa ataatttgct ggcaattatt aatgatgttc tcgacttctc gaaactggaa
    10381 gcaggtaagc tgattctgga aagtattcca ttcccactac gcagcacgct ggatgaagtc
    10441 gttactctgc tggcacattc ttctcacgat aaagggttgg aactgacgct caatattaaa
    10501 agcgacgtgc ctgataacgt gatcggcgac ccactgcgat tacagcaaat catcactaac
    10561 ctggtgggga atgcaattaa attcaccgag aatggcaaca ttgatattct ggtagaaaaa
    10621 cgtgcgctga gtaataccaa agtgcagatt gaagtgcaga ttcgggatac cggcattggt
    10681 attcctgaac gcgatcaatc gcgcttattc caggccttcc gacaggctga tgccagtatt
    10741 tcccgccgtc atggtggcac cggtctgggg ctggtgatta cacaaaaact ggttaatgaa
    10801 atgggcggcg atatttcgtt ccatagccag ccgaatcgcg gttcaacttt ctggttccac
    10861 attaatctcg atctgaaccc gaacattatt atcgaagggc catccaccca gtgcctcgcc
    10921 ggtaagcgcc tggcctatgt cgaaccaaac tccgcagcag cgcaatgcac gctggatatt
    10981 ttaagtgaaa cgccgctgga agtggtttat agcccaacgt tctccgcgct gcctcccgcg
    11041 cattacgaca tgatgttgtt aggcatcgcg gtgaccttcc gcgagccgct aacaatgcaa
    11101 catgagcgat tagcgaaagc ggtatcgatg accgatttcc tgatgctggc acttccttgc
    11161 catgcacaag tcaatgcaga aaaactcaag caagatggta tcggcgcgtg tctgctgaaa
    11221 ccattaacac ctacgcgcct gttgcctgcc ctgacggaat tttgtcatca caaacaaaac
    11281 acgcttttgc ctgtaaccga tgaaagcaag ctggcaatga cagtcatggc agttgatgac
    11341 aaccccgcta acctgaaact tatcggcgca ttgctggaag atatggtgca acatgtggaa
    11401 ctttgcgata gcgggcatca ggcggttgaa cgggcgaaac agatgccgtt cgatttgatc
    11461 ttaatggata ttcaaatgcc tgacatggat ggcattcggg cctgcgagct catccaccag
    11521 ctcccccatc agcaacaaac gccggttatc gcggtaacgg cgcatgcaat ggccgggcaa
    11581 aaagagaagc tgcttggcgc agggatgagc gattatctgg cgaaaccgat tgaagaagag
    11641 cgattgcata atttgttgtt gcgctacaag cctagcagcg gtatttcctc tcgcgtcgtg
    11701 acgcccgaag tcaacgaaat tgtggtgaac ccgaatgcga ccctcgactg gcaactggca
    11761 ctacgccagg cagcaggaaa aaccgattta gcgcgcgata tgctgcaaat gttactcgat
    11821 ttcctgcctg aagttcgcaa caaagttgag gaacagctgg ttggagaaaa cccggaaggc
    11881 ctggttgatt tgattcataa actgcatggc agttgcggct atagcggtgt gccgcgtatg
    11941 aagaatctct gccaactgat cgaacaacag ctacgtagcg gtactaaaga agaagatttg
    12001 gagccggagc tgctggaact gttggacgag atggataatg tagcgcgcga agccagcaaa
    12061 attctcgggt aacggacgtc ttgtcgagta gcaatcggct ttttatgacg ctggtttcag
    12121 attggtgaca aagtgcctgt gtttatgccg gatgcggcgt taacgcctta tccggcctac
    12181 aaaaccaatt aaattcaatg aattaaaaaa tgatgtagac ctgatacata gcacaacagg
    12241 ctattttgcg tttagcatca gtctcaagcc ggctccagat agagccggtt ttggttttct
    12301 gtcttaacgc accatgcacg ggcgcttgtt atcgaacgtc cagccaggaa tcagatactg
    12361 cattcccatc gcatcgtcac gcgcgccaag cccgtgtttc tgatacagct catgggcttt
    12421 catcacttga tccatatcga tttctacacc cagccccggt ttttccggca cctgaaccag
    12481 accgcctttg atctcaaacg gttctttggt caagcgctga ttgccttcct gccaaatcca
    12541 gtgcgtatca atcgcggtga ttttacccgg tgcagcggcg gcaacatggg taaacatcgc
    12601 cagggaaata tcgaagtggt tgtttgagtg tgaaccccag gtcaggccaa attcatggca
    12661 catttgcgcc acacgtaccg aaccttgcat cgtccagaaa tgcggatccg ccagcgggat
    12721 atcaacggat tgcagggaga gcgtatggcc catctgccgc cagtcggtgg cgatcatatt
    12781 ggttgcggtc ggcaggccag ttgcgcgacg gaactctgcc atcacttcac gcccggagaa
    12841 accttgctcc gcaccacacg gatcttctgc ataagccagc gaccctttca ggtatttacc
    12901 aattttaatc gcttcgttca gcgaccaggc accgttagga tcgagcgtaa tacgcgcctg
    12961 cgggaagcgt tgcgccagtg ccacaataga ctcggcctct tcttccccgg ccagtacacc
    13021 gcctttcagt ttgaaatcgt tgaagccata tttttcatac gccgcttccg ccaggcgcac
    13081 caccgcatcc ggcgtcatcg cttcttcatg acgcaggcga taccagtcgc atgagtcatc
    13141 cggctggctt tgatacggca gcggtgtggc tttgcgatca ccgacgaaga acagataacc
    13201 gagcatttcg acttcgctac gctgttgacc atcgcccagc agggatgcca cgtttacccc
    13261 cagatgctgc cccagcagat ccagcattgc cgcttctatc ccggtaacta catgaatagt
    13321 ggtacgcaaa tcaaatgtct gcaaaccgcg cccaccagca tcacgatcgg caaaagtatt
    13381 acgcaccagc gtcagaacgt ttttgtattc acccagcgtt ttacctacca ccagcggaat
    13441 cgcatcttcc agcgttttac ggattttctc gccgccggga atttccccta cgccagtgtg
    13501 accagaatta tctttgataa tcacaatatt acgcgtaaag aacggtgcgt gtgcaccact
    13561 cagattcatc agcatactgt catgacccgc caccgggatg acctgcattt cagtaacaac
    13621 aggcgtcgta aattgagaac tcatatttat gtcctttttc agaatcaatg acggccgaaa
    13681 acgggacgtt tacggtcaaa ggtccagccg gggatcaggt actgcatcgg acctgcgtcg
    13741 ttacgcgcac cgccaggcag acgtttatag gcctcatgtg ccttttgtac ctgttcccag
    13801 tccagttcca cgcccagacc gggcgcatca ggaacggcaa tttttccgtt tttaatctcc
    13861 agcggatttt tggtcaggcg acaatcgccc tcctgccaaa tccagtgggt atcgatagcg
    13921 gtaggattac ccggtgccgc cgcgcccaca tgggtaaaca tcgccagaga gatatcgaaa
    13981 tggttattag aatggcagcc ccaggtcagc ccccagtcgt cgcaaagctg cgccacacgg
    14041 actgcaccgg aaagcgtcca gaagtgcgga tcggcaagtg gaatatctac cgcattgagc
    14101 atcaccgcat gacccatttc gcgccagttg gtggcgatca tgttagtcgc gacgggcaag
    14161 ccggtcgccc gccgaaactc cgccataact tcacgaccgg agaaaccctg ttctgcgccg
    14221 catggatctt cggcataggt aagaacatca ttcagccctt tgcatagaga aatggcttca
    14281 tcaagtagcc aggcaccgtt gggatcaacg gtaatccgcg catcagggaa gcgtttcttc
    14341 aatgcacgaa cagtgtcgat ttcttgctcg ctaggtaaca ccccgccctt aagtttgaaa
    14401 tctttaaagc catagcgatc ctgtgaggct tccgccagac gcacaacggc ttcgctgttc
    14461 atcgctttct gatggcgcaa ctgataccac tcatggttgc ccggcgtatt ttccagataa
    14521 ggaagatcgg ttttggtccg atcaccgata taaaacagat aaccgaggac ggtaatagtc
    14581 tcgcgttgct tgcctggccc caacagttcg cagaccggaa cattcagcgc cttacctagc
    14641 aggtcaagca aggcggcttc cagcgccgcc acggcattaa cgcgcaattc aaaagtccag
    14701 gcacctttgc cgaaggtatc aaaatcggct gcctgattac ctttatgcac ctgctgaacc
    14761 actttattca ggcgcgcaac ttcctggccc agaaccatcg gaatagcatc gaccagtgtt
    14821 tgataaatca cctctccgcc cggcgcttca ccaatgccgg tatgcccggc gttatcggtg
    14881 agtaccacaa tattgcgggt gaaatatgcg ttatgtgccc caccaatatt aagcaacatg
    14941 ctatcgtgcc cggcgaccgg aatgactttc atatcagtaa taacaggact ggattgtgtc
    15001 gtcatcttat tgccccgcga caggtttcag ctcgatacgc ttgatatcgc ccaccagcac
    15061 caggtagctc agtaccgcga ttaaggcatg aacaccaaca taaatcagcg ccccattaaa
    15121 ggagccagtc gtgccaacga tataaccaat tgcgattggc gtgacgatgc cagaaatgtt
    15181 accgaacatg ttgaacaggc caccggaaag accgctgatc tcttttggcg cggtatctgc
    15241 cattactgcc caacccagcg ccccgatgcc cttaccgaag aaggccagcg ccataaaacc
    15301 gatgatcatc cactcaacgt tgacgtagtt gcagaacacc atcaccatcg acagcaacat
    15361 gcccattacg atcggtgttt tacgcgcaat gttcagcgat cccgtgcggc gcatcagcca
    15421 gtcggaaata atcccaccca gcacaccgcc gataaaaccg caaaccgccg gaacggaagc
    15481 cacaaagccc gctttcagaa tcgacatccc acgcgcctgc accagataaa ccggaaacca
    15541 ggtaatgaaa aagtaagtca gggcgttgat acagtactga ccgatataaa cgccgatcat
    15601 catccgtgac ccgagcagtt gtttgatctg cccccacttc acgctgaacg gcactttaac
    15661 tttggtgttt tgctgatcca tattgatcag cgcgccaccc gcggcgatgt actccagctc
    15721 tttctgattt acccccggat gttgatttgg ctcgtggatg actttcaacc agataaagct
    15781 gatgacaatc cccagaccgc ccataaagaa gaagacgtgt gaccagccca cttcatgcgt
    15841 cagccagccc ataatcggcg cgaagatcac cgttgcgaag tattgagcgg agttaaaaat
    15901 cgacaccgcc gttccccttt cctgcgccgg aaaccaggcc gcaacaatgc gactgttgcc
    15961 ggggaaagat ggcgcttcag caagcccgac caggaagcgc agagtaaaca gggcaacgat
    16021 aatgccgaat ccactaaaga tatcgacgaa gccttgcagc aaggtaaaca tcgaccagat
    16081 aaagatcgac cagaagtaga cgcgttttga accaaaacga tccagcaacc aaccaccagg
    16141 gatctgcccg ataacataag cccatgagaa ggcagagaac acatagccca ttcccacggg
    16201 atcaaggccg atatctttgg ccatttccga accggcaata gaaagcgtag cgcggtcgcc
    16261 gtagttgaag gatgtgacga taaacaacat caccactatc cagtaacgag cattcgtgcg
    16321 tttttccaca ctgctcgcag cctgacttaa agaactcatt gttgcactcc tgaaaattcg
    16381 cgttagccac gctcactctg gactgcgaca tagccaggaa atcagaggtg acgtagggtg
    16441 ttttttgccg tttttatagg tcgttcgccg aatacggcgc gtgtttatat ctggcaatag
    16501 cagtataaaa agcgcgccat agcggctcac cgtgcaacaa cacaacatta atgcgttcaa
    16561 tgaggcccga ttttggcatt agccctggac ggtggaatcc acttcacgga aatgaaaaca
    16621 agaacaagaa aggaagggtt aaaacgaaga aataaagaag agtatgaaat ggatcgcttg
    16681 actccaggca aacgccagta aaaatccgcg ctatgaagca gtttttactg gcatttgcct
    16741 gaaaagattc gattcagcac cgctaaaacg acatttaccg ctcgctgaac atatcaggac
    16801 aacagcgtgc cccactgttc gacccacgga tttgattccg tttccggttc cgggttctca
    16861 ctggcgtcaa tcaacagtat ttcgccaacc cgctgagcgc tctgttcctg caacaaggca
    16921 tcgaactgtt tgccgccatt gcagaaattt acataactgc tgtcgccgag cgcaatcacg
    16981 ccataacgca gattcggctg gaagcccaga ctatctttga ttccctgaaa gagcggcaca
    17041 atgctatcag gaaggtcgcc ctgcccggtc gtggacgtaa ccaccagaac atacttatcc
    17101 tgataaggca gccagtcgct taattcagga tcttcaaata ccgttgcttt gtggccctgt
    17161 gcggtcagaa ttgcttctgc ttcttccgcc actaacagcg aatttccgta catggtgccg
    17221 acaaaaatac caatttccgc cataccgttt tctccctgga ttaggaactt atctctccat
    17281 cctgacccga tggcgcactg aactcaaccc tttcattttc aggaagcaga ccgcgccagc
    17341 caaattgtga taacgcctgc atccaggtgt cgtccaggcc cgcgtgaata gtcagcggct
    17401 cgccagtaaa aggatgcgtc aatgacaact gactggcatg tagcattaac cgctggaggc
    17461 caaaatgctc agcaccgctg cgattctggc gtaaatcgcc atgtttgcta tcgccaataa
    17521 tcggatgacg caaatgggca agatgtcggc gaagctgatg tttgcgtccg gttttcggct
    17581 ccagttccac caggccgtag cgcgtggtcg ggtaacgtcc ggtcgctacc ggcatttcta
    17641 cggtcgccag accgcgataa tgcgtcactg ctggctgcgg gcctttatct tcgcgggcaa
    17701 atttatcagc gattttgtcc agttcttcca ccagtggata atccagcacc gcttcttcca
    17761 tcaaccagcc gcgcacaatc gcatggtaac gtttctggat ttggtgctgt tcaaactgtt
    17821 gtgccagcag ccgtccggcc tcgctggata atcccatcaa caacacacca gaagtgggtc
    17881 ggtccagacg atgagcagta aaaacatgct gtcctatctg gtcacgcacg gtttgcatga
    17941 ccactacttt ctcgtcgcga tccagccagc tacggtgaac cagccagccg gagggtttat
    18001 ttaccgcaac cagccattca tcctgataga gtatttccag cattagctcg catcatccgc
    18061 aaaaagagca tccagttttt ccaactcagc cagaataagc gcgcgttgcg gatggtccgt
    18121 cgccagcgcc atttcataat agggtgcaac agcaaaagcg cccggtaacg gctggttgtt
    18181 atttaacaaa tcgtgcattc gcgggatcag cacccactgc aaccactcca gtggttccat
    18241 ggtgtccata aagaacggtt gggtactatt aaattgatgc ggctggggtt catcgtttcg
    18301 ccagtgctga tgttcacgca gtaacgcttc aagcgcctgc aactggagac gaacgcggtc
    18361 atgagtggtc ataaagcaac ctcaataaga aaaactgccg cgaaggatag caggaataaa
    18421 aaaagggagc actgtattca cagcgctccc ggttcgtttc gcagcattcc agctactttt
    18481 gttgctccct gctcatcctt gacaactttt cctctggcct tgcggccaat cgttcatcct
    18541 gaactattgc ttcctgctca caccaccccg atgtgatact tcatcctgaa gtgtccctgg
    18601 ccttcctgac ccaccgaatc atcctgaccg gttctcattc tccatcctgg aggtgtcctt
    18661 taacgcgtcc tgcgtcatcc tcttcgcttc atccagaagc ctttccctga aacaccatcc
    18721 tggtgtgtcc tgcagaagtg tcatcatcct gatgttcact tcgttgtctg actccctgtc
    18781 gacgaagata gaatcgtctt tttcgcgaag tcttacaagg cgcttacaga caatgcctta
    18841 aggaaaattt catatgaaag ttaagaacta acaatcaaca ttttgatttt aaatgaaaat
    18901 aacgattctt atctttttaa gtattcgctt atttcctggc gatctcctac agagtctgta
    18961 agagatctct cacaaccaga tggggtattt ggattacaga agaggcttaa gttgattaag
    19021 gaattccgca agatttgagg ctaaatgcgt ccgtttacgt gttcccagcg tctctttgca
    19081 cacttcaccg gagagattac acaccgagat gacctctaac tcttcttcca gcgtcgcgat
    19141 aaatagcgtg ggcggcagtt ttagcctttt ctgagtcacc agatgaccaa tcagattttc
    19201 ctgcacccga cggaagtcgt cttcactcca ggtttgcagc aatgtcagtt tgatatcgcc
    19261 aaactgcgcg tgcatatccc cggcaaactg agtcgtataa aaggtatgaa ttgtgggttg
    19321 tatcacaata tcaaaagcgc gttcaaccgc gtttacattt tgttccccgg taaacggctg
    19381 gggttgccag tatacggcat cttcggtagt tgaaataatg cacggtgaag gaacaccgta
    19441 cagttcctca cttaacggcc agcttttatg ctcttcatgc cacgcatcgc agtagcgtgc
    19501 agtaaaatct ttcagggctt gtgcggtcaa atcgtccact aatttctctc ttcacttaaa
    19561 ccagatacac ttgtcgttta gtttatctgg tttatgacgg tgaaacatgt cttcttatgc
    19621 aaaccatcag gcacttgcgg gcctgactct tggaaaatca accgattacc gggataccta
    19681 tgacgccagc ctactgcaag gcgttccacg cagcctgaat cgcgacccgc tgggtctgaa
    19741 agcggataac ctgccttttc acggtacgga tatctggacg ctgtatgaac tttcctggct
    19801 gaatgcgaaa ggtttgccgc aggtcgctgt cggtcatgtt gaacttgatt acaccagcgt
    19861 aaatctgatt gagtcgaaga gttttaagct ctatctcaac agttttaacc agacgcgttt
    19921 taataactgg gatgaggtgc gccagacgct ggagcgcgac ttaagcactt gcgctcaggg
    19981 taagattagc gtggcgttat atcgtcttga tgaactggaa ggccagccga taggtcattt
    20041 taatggcact tgcattgatg accaggatat cactatcgat aactatgaat tcactactga
    20101 ctatctggag aatgccacct gtggtgaaaa agtagtggaa gagacgcttg tcagccacct
    20161 gctgaaatca aactgcctga tcacccatca accagattgg ggttcgctcc aaattcagta
    20221 tcgtggacgc caaattgaca gagaaaaact gctgcgttac ctggtctcat tccgtcatca
    20281 caacgagttc cacgaacagt gcgtggaacg catctttaat gacctgttac gcttctgcca
    20341 gccagaaaaa ttgagcgttt acgcacgtta tacccgtcgt ggcggtctgg acattaaccc
    20401 gtggcgcagt aatagcgatt ttgtcccatc gaccacaaga ctggttcggc aataaatttt
    20461 ttctcaattt tgcgtgctgg attcacgcag aaggttgtga aaggtcatca ggcagggcta
    20521 ttgtaatcaa agggaatgac gatattcgtc ccataaggag tttttcttga ttacacatat
    20581 tagcccgctt ggctccatgg atatgttgtc gcagctggaa gtggatatgc ttaaacgcac
    20641 cgccagcagc gacctctatc aactgtttcg caactgttca cttgccgtac tgaactccgg
    20701 tagtttgacc gataacagca aagaattgct gtctcgtttt gaaaatttcg atattaacgt
    20761 cttgcgccgt gaacgcggcg taaagctgga actgattaat cccccggaag aggcttttgt
    20821 cgatgggcga attattcgcg ctttgcaggc caacttgttc gcggtcctgc gtgacattct
    20881 cttcgtttac gggcaaatcc ataacaccgt tcgttttccc aacctgaatc tcgacaactc
    20941 cgtccacatc actaacctgg tcttttccat cttgcgtaac gctcgcgcgc tgcatgtggg
    21001 tgaagcgcca aatatggtgg tctgctgggg cggtcactca attaacgaaa acgagtattt
    21061 gtatgcccgt cgcgtcggaa accagctggg cctgcgtgag ctgaatatct gcaccggctg
    21121 tggtccggga gcgatggaag cgccgatgaa aggtgctgcg gtcggacacg cgcagcagcg
    21181 ttacaaagac agtcgtttta ttggtatgac agagccgtcg attatcgccg ctgaaccgcc
    21241 taacccgctg gtcaacgaat tgatcatcat gccagatatc gaaaaacgtc tggaagcgtt
    21301 tgtccgtatc gctcacggta tcattatctt ccctggcggt gtgggtacgg cagaagagtt
    21361 gctgtatttg ctgggaattt taatgaaccc ggccaacaaa gatcaggttt taccattgat
    21421 cctcaccggc ccgaaagaga gcgccgacta cttccgcgta ctggacgagt ttgtcgtgca
    21481 tacgctgggt gaaaacgcgc gccgccatta ccgcatcatc attgatgacg ccgctgaagt
    21541 cgctcgtcag atgaaaaaat cgatgccgct ggtgaaagaa aatcgccgcg acacaggcga
    21601 tgcctacagc tttaactggt caatgcgcat tgcgccagat ttgcaaatgc cgtttgagcc
    21661 gtctcacgag aatatggcta atctgaagct ttacccggat caacccgttg aagtgctagc
    21721 tgccgacctg cgccgtgcgt tctccggtat tgtggcgggt aacgtaaaag aagtcggtat
    21781 tcgcgccatt gaagagtttg gtccttacaa aatcaacggc gataaagaga ttatgcgtcg
    21841 tatggacgac ctgctacagg gttttgttgc ccagcatcgt atgaagttgc caggctcagc
    21901 ctacatccct tgctacgaaa tctgcacgta atctcccctc ccccggtgac gtttgttgcc
    21961 gggttttctc gttttttgtc acttactcat caactcattt catttgttat atgaatgttt
    22021 cttaccaccc tcacgcgaca aatatcatca cagttaatat gtcatacaat ttatgttgca
    22081 acgcaaacgt tcccctattt tcataaaccg tattttatcg ctatgaaaaa gaaattatcg
    22141 ccatgattaa ctaaaagtat tgattttttc agttcaacct acatatattg cgcgccccgg
    22201 aagaagtcag atgtcgttta atgggcaaat attgccctta aattctattt tacttttgat
    22261 ttacagagta aagcgttggg ataatctatc ttccaagtag attattgtat ttgagatcaa
    22321 gatcactgat agatacataa cttgtgtgta tctttccgcc ctcaaattat tacggcggta
    22381 aatgattaag ccatcgccga tagacagatt tcatttttac ggtcaggcac cttcccgggc
    22441 tgaactggct aaaagctgaa ttatttgcat tcctccagga gaaatagatg gaaacgactc
    22501 aaaccagcac gattgcgtcg aaagactctc gtagtgcctg gcgcaagaca gacaccatgt
    22561 ggatgctggg cctttacggc acggcaatcg gcgcgggcgt gctgttcctg ccaatcaacg
    22621 ccggtgttgg cggtatgatc ccgctgatca tcatggctat ccttgcgttc ccgatgacat
    22681 tttttgcaca ccgcggcctg actcgcttcg tactgtctgg taaaaacccg ggcgaagaca
    22741 tcaccgaggt tgtagaagaa cactttggta ttggcgcagg taaactgatt accctgctct
    22801 atttcttcgc tatctacccg atcctgctgg tttatagcgt agcaatcacc aatacagttg
    22861 aaagcttcat gtctcaccag ctgggtatga caccaccgcc gcgtgcgatt ctgtcgctga
    22921 tcctgatcgt gggtatgatg accatcgttc gcttcggtga gcagatgatc gttaaagcga
    22981 tgagtattct ggtattcccg tttgttggcg tactgatgct gctggctctg tacctgatcc
    23041 cgcagtggaa cggcgcagca ctggaaacgc tgtctctgga cactgcatct gcaaccggga
    23101 acggtctgtg gatgaccctg tggctggcaa ttccggtaat ggtgttctcg ttcaaccact
    23161 ctccgatcat ctcttctttc gccgttgcga agcgtgaaga gtacggcgat atggcagaac
    23221 agaaatgctc gaagatcctt gcattcgcac acatcatgat ggtgctgacc gtaatgttct
    23281 tcgtcttcag ctgcgtactg agcctgactc cggcagacct ggctgcagct aaagagcaga
    23341 acatctcgat tctgtcttac ctggctaacc actttaacgc accggttatc gcgtggatgg
    23401 ctccgattat cgcgattatc gctatcacca aatccttcct cggccactac ctgggcgcac
    23461 gtgaaggctt caacggtatg gtgattaaat ctctgcgtgg taaaggtaag tctatcgaaa
    23521 tcaacaagct gaaccgtatc actgcgctgt tcatgctggt aacgacctgg attgttgcca
    23581 ccctgaaccc gagcatcctg ggtatgattg aaaccctggg cggcccaatc atcgcgatga
    23641 tcctgttcct gatgccgatg tacgcaattc agaaagtacc ggcaatgcgt aagtacagcg
    23701 gtcacatcag caacgtattc gttgtcgtga tgggtctgat tgcaatctcc gcaatcttct
    23761 actctctgtt cagctaagtc ctttcgcgcc gctttcgggc ggcgcgtcct ccgttttaac
    23821 gcgatgtatt tcctatgatt agcgtattcg atattttcaa aatcggcatt ggcccttcca
    23881 gttctcatac cgttggacca atgaaagcgg gtaaacaatt taccgacgat ctgattgccc
    23941 gtaacctgct taaagacgtg acccgcgtgg tggttgacgt gtacggctcg ctctctctga
    24001 ctggtaaagg ccaccacact gatatcgcca ttattatggg cctagcgggt aacctgccag
    24061 atgccgtgga tatcgattcc atccccggtt ttattcagga tgtgaatact catggtcgcc
    24121 tgatgctggc aaacggtcag catgaagtgg agttcccggt tgatcagtgc atgaacttcc
    24181 acgccgacaa cctttctctg catgaaaacg gtatgcgcat taccgcgctg gcgggcgata
    24241 aagtcgttta cagccagact tactactcta ttggcggtgg ctttatcgtt gatgaagagc
    24301 attttggcca gcaggatagc gcaccggttg aagttcctta tccgtacagt tcagcagccg
    24361 atctgcaaaa acattgtcag gaaaccgggc tgtcactctc tggcctgatg atgaaaaacg
    24421 agctggcgct gcacagcaaa gaagagctgg aacagcacct ggcgaacgtc tgggaagtca
    24481 tgcgcggcgg tattgagcgc ggtatttcta ccgaaggcgt gttgcctggc aaactgcgcg
    24541 ttccacgccg tgctgcggca ctacgccgga tgctggtcag ccaggataaa accaccactg
    24601 acccgatggc ggtagttgac tggatcaaca tgtttgcact ggcagtgaac gaagagaacg
    24661 ctgctggcgg acgcgtggtg actgcgccga ctaacggtgc gtgcgggatt atcccggctg
    24721 tgctggcgta ctacgataag tttatccgcg aagtgaacgc taactcactg gctcgttacc
    24781 tgctggtagc cagtgccatt ggttctcttt ataagatgaa cgcgtcaatt tctggtgctg
    24841 aagtcggctg ccagggtgaa gttggtgtgg cgtgctcaat ggcggcggct ggtctggcag
    24901 aactgttagg tgcaagcccg gcgcaggtgt gcatcgcggc ggaaatcgcc atggagcaca
    24961 acctcggtct gacgtgtgac ccggtcgccg gacaggtcca ggtgccatgc atcgagcgta
    25021 acgccattgc ggcagtaaaa gcggtgaacg ccgcacgtat ggcgctacgc cgtaccagcg
    25081 agccgcgcgt ctgcctcgat aaagttatcg aaaccatgta cgaaacaggt aaagatatga
    25141 acgccaagta ccgcgaaacc tctcgcggcg gcctggcaat gaagatcgtt gcctgcgatt
    25201 aatcgctctc cagaggcctc gttttgcgag gcctcttccc gatttctcat ccagccgtag
    25261 cctgttccgg catcgaatgt tacccttatc gcctgatctt taagggggtt atcgtggctg
    25321 ttcatttgct tattgtcgat gcactgaatc ttattcgtcg cattcatgcc gttcagggat
    25381 cgccctgtgt cgaaacctgc cagcatgcgc tcgatcagct cattatgcac agccagccaa
    25441 cccacgcggt cgccgttttt gatgatgaaa accgcagtag cggctggcgt catcagcgtt
    25501 taccagatta caaagcgggt cgaccgccaa tgccggaaga gttgcacgac gagatgcctg
    25561 cattacgcgc cgcctttgag caacgcggcg tcccgtgctg gtcaaccagc ggcaacgaag
    25621 ccgatgactt agccgccacg ctggcggtca aagtgacaca ggccgggcat caggcaacga
    25681 ttgtttcgac agataaaggc tactgtcagt tactttcacc gacattacgt attcgtgatt
    25741 acttccagaa acgttggctg gatgcgccat ttatcgataa agaatttggc gttcaaccgc
    25801 agcagttgcc cgattactgg ggacttgcgg ggatcagcag ttcaaaggta ccgggtgttg
    25861 cgggaatcgg accaaaaagc gccacgcagc tgctggtcga gtttcagagt ctggaaggga
    25921 tatatgagaa tctggatgcg gttgccgaaa agtggcgcaa aaaattagaa ccccataaag
    25981 agatggcgtt tctgtgccgc gatattgccc gcttacaaac cgatttgcat atcgacggca
    26041 atttacagca attgcggttg gtacggtaac ggcgagccgg atacgccgca aacgtcgtat
    26101 ccggcattat cacatcagcg catttaccag gcggtatggt aaagctctac aatatcctca
    26161 agcgttgctt cacgcgggtt gccaccggta caaacatcat ccagtgccgc ctgcgccagt
    26221 gccggaatgt cttccttgcg tacaccaaca tcacgcaaat gtggcggaat accgacatca
    26281 cggttgagag caaacaccgc ttcaacagcg gcattacgcg cctcttcctg gctcatacct
    26341 tccactttca cgcccataac gcgcgcgata tcgcggtact tctcaccggt aaagtcagcg
    26401 ttatagcgca tgacatgcgg taacaggatg gcgttcgcaa caccgtgtgg agtgttgtaa
    26461 aacgcgccca gtggatgcgc cataccatgc accaacccta acccaacatt cgagaagccc
    26521 atacccgcaa catactgccc gagcgccatt tcttctccgg catccttatc accagcaacc
    26581 gatcctcgca gcgccccagc aatgatttca atcgctttaa tgtgcagtgc atcggttagc
    26641 gcccacgcgc cacgggtaat atacccctca atagcatgag tgagcgcatc gacacccgtc
    26701 gcagctttca gcgctggagg cataccatcc atcatgtcag cgtcaataaa cgccacctgc
    26761 gggatatcat gcggatcaac gcaaacaaac ttgcgccgtt tctcttcgtc agtgatcacg
    26821 tagttaatgg tcacttctgc cgcagtacct gctgtggtag gaattgccag aatcggtaca
    26881 ctgggtttat tggtcgggga aagcccttcc aggctatgca catcggcaaa ctccgggttg
    26941 ttgctgataa tgccaatcgc tttacaggta tcctgtggag aaccaccacc aatagcgatc
    27001 aggtaatccg cgccgctatt ctggaataca ccgagccctt ctttgacgac agtaattgtt
    27061 gggttgggca ctacgccgtc gtaaatcgcc catgccagcc ctgcagcatc catcttatcg
    27121 gtcactttcg ccaccacgcc gcattgcacc agcgttttat cggtgacgat cagcgccttc
    27181 tgataaccac ggcgtttcac ctcatcggtt aaagccccaa cagcaccccg accaaaccat
    27241 gccgtttcgt tcagaatcat tctgttagcc atcatccttc tccttgttgc tttacgaaat
    27301 tactcttcaa tccgtaaccc ataggttttg aatttctcca gcactacggc aatctcttca
    27361 tcgctcagca ctggcaccgg gtccgtaatc gccagggtcg tcaggtaaag ttgcgccagc
    27421 acttcaactt catgcgccag ccataacgct ttttccagat tcacctcaca agcgataagc
    27481 ccatgatgtt gtaacaaagt tgccttacga tttttgagag ccagcgcaac atgttcagaa
    27541 agttcgcgtg ttccaaaggt cgcataaggc gcgcaaggaa tagaattacc acctgccgcc
    27601 gcaatcatgt agtgaatagc ggggatcggt cggttaagaa tggaaactgc tgtgcaatga
    27661 acggcatgat tgtgaacaac cgcgttggca tccggtctgc tttgataggc cgccatatgg
    27721 aaacgccatt cgcttgaggg gagctttcct tcctcatgtt taccgttgcc atcaataaag
    27781 acaatatgcg actccgtcag tttttcatat ggaatgcctg taggcgtaat cagcatccca
    27841 tcctgataac gtacactgac gttccccgct gtcccctggt tcagtcccag gcgggtcatt
    27901 tccaggcaag tgtcaataat ctgacgagca agtttatttc gttccattag ctacctctct
    27961 ctgattcaaa acagggcaat aatgttgttc ctttcacact attgaattag ccgtttaatt
    28021 acccaccatc ttcttcctga ttaaccagaa agaaattcac aagcttatat tttgtgacct
    28081 ggttcaacta atcacagtaa ataactgcaa gttctctttt tataacccca ttaaaaatga
    28141 ccgctcttaa aaatatttat caaaacggtc atttttctat tcactcaagc taggaatgcc
    28201 cgttttcggc ataaacaatt aatacggtca tctggtttgt gttttttatg atttattttc
    28261 tgaaacgggc atgaaatttc gattattaaa gtgatggtag tcacataaag tcaccttcca
    28321 gctaataagt gtgaccgccg tcatattaca gagcgttttt tatttgaaaa tgaatccatg
    28381 agttcatttc agacaggcaa atattcactg atatgaagcc cgaactcgct ggttttgcac
    28441 ttttgaaaac ataaccgatt acgtgcttaa gcttctgaac ctaagaggat gctatgggaa
    28501 acacatcaat acaaacgcag agttaccgtg cggtagataa agatgcaggg caaagcagaa
    28561 gttacattat tccattcgcg ctgctgtgct cactgttttt tctttgggcg gtagccaata
    28621 accttaacga cattttatta cctcaattcc agcaggcttt tacgctgaca aatttccagg
    28681 ctggcctgat ccaatcggcc ttttactttg gttatttcat tatcccaatc cctgctggga
    28741 tattgatgaa aaaactcagt tataaagcag gaattattac cggattattt ttatatgcct
    28801 tgggcgctgc attattctgg cccgctgcag aaataatgaa ctacacctta tttttagttg
    28861 gcctatttat tattgcagcc ggattaggtt gtctggaaac tgccgcaaac ccttttgtta
    28921 cggtattagg gccggaaagc agtggtcact tccgcttaaa tcttgcgcaa acatttaact
    28981 cgtttggcgc aattatcgcg gttgtctttg ggcaaagtct tattttgtct aacgtgccac
    29041 atcaatcgca agacgttctc gataaaatgt ctccagagca attgagtgcg tataaacaca
    29101 gcctggtatt atcggtacag acaccttata tgatcatcgt ggctatcgtg ttactggtcg
    29161 ccctgctgat catgctgacg aaattcccgg cattgcagag tgataatcac agtgacgcca
    29221 aacaaggatc gttctccgca tcgctttctc gtctggcgcg tattcgtcac tggcgctggg
    29281 cggtattagc gcaattctgc tatgtcggcg cacaaacggc ctgctggagc tatttgattc
    29341 gctacgctgt agaagaaatt ccaggtatga ctgcaggctt tgccgctaac tatttaaccg
    29401 gaaccatggt gtgcttcttt attggtcgtt tcaccggtac ctggctcatc agtcgcttcg
    29461 caccacacaa agtcctggcc gcctacgcat taatcgctat ggcactgtgc ctgatctcag
    29521 ccttcgctgg cggtcatgtg ggcttaatag ccctgacttt atgcagcgcc tttatgtcga
    29581 ttcagtaccc aacaatcttc tcgctgggca ttaagaatct cggccaggac accaaatacg
    29641 gttcgtcctt catcgttatg accatcattg gcggcggtat tgtcactccg gtcatgggtt
    29701 ttgtcagtga cgcggcgggc aacatcccca ctgctgaact gatccccgca ctctgcttcg
    29761 cggtcatctt tatctttgcc cgtttccgtt ctcaaacggc aactaactga acatattttc
    29821 cgaataaagt gaggaatctg taatgaaaaa aatcagctta ccgaaaattg gtatccgccc
    29881 ggttattgac ggtcgtcgca tgggtgttcg tgagtcgctt gaagaacaaa caatgaatat
    29941 ggcgaaagct acggccgcac tgctgaccga gaaactgcgc catgcctgcg gagctgccgt
    30001 cgagtgtgtc atttccgata cctgtatcgc gggtatggct gaagccgctg cttgcgaaga
    30061 aaaattcagc agtcagaatg taggcctcac cattacggta acgccttgct ggtgctatgg
    30121 cagtgaaacc atcgacatgg atccaacccg cccgaaagcc atttggggct ttaacggcac
    30181 tgaacgtccc ggcgctgttt acctggcagc ggctctggca gctcacagcc agaaaggcat
    30241 cccggcattc tccatttacg gtcatgacgt tcaggatgcc gatgacacat cgattcctgc
    30301 cgatgttgaa gaaaaactgc tgcgctttgc ccgcgccggt ttggccgtcg ccagcatgaa
    30361 aggtaaaagc tatctgtcgc tgggcggcgt ttcgatgggt atcgccggtt ctattgttga
    30421 tcacaacttc tttgaatcct ggctgggaat gaaagtccag gcggtggata tgaccgaact
    30481 gcgtcgccgt atcgatcaga agatttacga cgaagccgaa ctggaaatgg cactggcctg
    30541 ggctgataaa aacttccgct atggcgaaga tgaaaataac aaacagtatc aacgcaatgc
    30601 cgagcaaagt cgcgcagttc tgcgcgaaag tttactgatg gcgatgtgta tccgcgacat
    30661 gatgcaaggc aacagcaaac tggccgatat tggtcgcgtg gaagaatcac ttggctacaa
    30721 cgccatcgct gcgggcttcc aggggcaacg tcactggacc gatcaatatc ccaatggtga
    30781 caccgccgaa gcgatcctca acagttcatt tgactggaat ggcgtgcgcg aaccctttgt
    30841 cgtggcgacc gaaaacgaca gtcttaacgg cgtggcaatg ctaatgggtc accagctcac
    30901 cggcaccgct caggtatttg ccgatgtgcg tacctactgg tcaccagaag caattgagcg
    30961 tgtaacgggg cataaactgg acggactggc agaacacggc atcatccatt tgatcaactc
    31021 aggttctgct gcgctggacg gttcctgtaa acaacgcgac agcgaaggta acccgacgat
    31081 gaagccgcac tgggaaatct ctcagcaaga ggctgacgct tgcctcgccg ctaccgaatg
    31141 gtgcccggcg atccacgaat acttccgtgg cggcggttac tcttcccgct tccttaccga
    31201 aggcggcgtc ccgttcacca tgactcgtgt caacatcatc aaaggcctgg gaccggtact
    31261 gcaaatcgcg gaaggctgga gcgtggaatt gccgaaggat gtgcatgaca tcctcaacaa
    31321 acgcaccaac tcaacctggc caaccacctg gtttgcaccg cgcctcactg gtaaagggcc
    31381 gtttacggat gtgtattcgg taatggcgaa ctggggtgcc aaccacggag ttctgaccat
    31441 cggccacgtt ggcgcagact ttatcactct tgcctccatg ctgcgtatcc cggtatgtat
    31501 gcacaacgtt gaagagacca aagtgtatcg tccttctgcc tgggctgcgc acggcatgga
    31561 tattgaaggc caggattacc gcgcttgcca gaactacggt ccgttgtaca agcgttaata
    31621 cattactccc tgatgtgatg cccggtcgct ccggctaccg ggcctgaaca agcaagagtg
    31681 gttagccgga taaacaatgt tatccggcta tattgcagga gcgattatga aacaagaagt
    31741 tatcctggta ctcgactgtg gcgcgaccaa tgtcagggcc atcgcggtta atcggcaggg
    31801 aaaaattgtt gcccgcgcct caacgcctaa tgccagcgat atcgcgatgg aaaacaacac
    31861 ctggcaccag tggtctttag acgccatttt gcaacgcttt gctgattgct gtcggcaaat
    31921 caatagtgaa ctgactgaat gccacatccg cggtatcgcc gtcaccacct ttggtgtgga
    31981 tggcgctctg gtagataagc aaggcaatct gctctatccg atcattagct ggaaatgtcc
    32041 gcgaacagca gcggttatgg acaatattga acagttgatc tccgcacagc ggttgcaggc
    32101 tatttctggc gtcggagcct ttagtttcaa tacgttatat aagttggtgt ggttgaaaga
    32161 aaatcatcca caactgctgg aacgcgcgca cgcctggctc tttatttcgt cgctgattaa
    32221 ccaccgttta accggcgaat tcactactga tatcacgatg gccggaacca gccagatgct
    32281 ggatatccag caacgcgatt tcagtccgca aattttacaa gccaccggta ttccacgccg
    32341 actcttccct cgtctggtgg aagcgggtga acagattggt acgctacaga acagcgccgc
    32401 agcaatgctc ggcttacccg ttggcatacc ggtgatttcc gcaggtcacg atacccagtt
    32461 cgcccttttt ggcgctggtg ccgaccaaaa tgaacccgtg ctctcttccg gtacatggga
    32521 aattttaatg gtccgtagcg cccaggttga tacttcgctg ttaagtcagt acgccggttc
    32581 tacctgcgaa ctggatagcc aggcagggtt gtataaccca ggtatgcaat ggctggcatc
    32641 cggcgtgctg gaatgggtga gaaaactgtt ctggacggct gaaacaccct ggcaaatgtt
    32701 gattgaagaa gctcgtctga tcgcacctgg cgcggatggt gtgaaaatgc agtgtgattt
    32761 attgtcgtgt cagaacgctg gctggcaagg agtgacgctt aataccacgc gggggcattt
    32821 ctatcgcgcg gcgctggaag ggttaactgc gcaattacag cgcaatctac agatcctgga
    32881 aaaaatcggg cactttaaag cctctgaatt attgttagtc ggtggaggaa gtcgcaacac
    32941 attgtggaat cagattaaag ccaatatgct tgatattccg gtaaaagttc tcgacgatgc
    33001 cgaaacgacc gtcgcaggag ctgcgctgtt cggttggtat ggcgtagggg aatttaacag
    33061 cccggaagaa gcccgtgcac agattcatta tcagtaccgt tatttctacc cgcaaactga
    33121 acctgaattt atagaggaag tgtgaaatgc tgaaaacaat ttcgccgtta atttctcccg
    33181 aactattgaa agtgctggca gagatgggac atggagatga aattattttt tccgatgctc
    33241 actttcccgc ccattcgatg ggaccgcagg tgatccgcgc tgatggcctg ttggtgagcg
    33301 acttgctcca ggcgattatc ccattatttg agctggacag ttatgccccg ccgctggtga
    33361 tgatggcagc ggtagaaggt gacactctcg atcctgaagt agaacgacgt taccgtaatg
    33421 cgctttcact acaagccccg tgtcctgaca tcatccgcat caatcgtttt gcgttttatg
    33481 aacgggcgca aaaagccttt gcgatcgtta tcacaggcga acgagcgaag tacgggaata
    33541 ttcttttaaa aaaaggggta acaccgtaat ctcataccgg tacgcccgca tgacgcgggc
    33601 ggttatcgaa tgatggggtg aaaaatatga aagcggcacg ccagcaagcg atagtcgacc
    33661 tgctgctgaa ccataccagc ctgaccacgg aagctctctc tgaacagcta aaggtcagta
    33721 aagaaaccat tcgtcgcgat ctcaatgaat tacagacgca gggtaaaatt ctgcgcaatc
    33781 atggacgcgc taaatatatc caccgtcaaa atcaagacag tggcgatccc tttcacatca
    33841 ggctgaaaag ccattatgcg cataaagcag atatcgcgcg cgaggcgctc gcgtggattg
    33901 aagaagggat ggtgatagcc ttagacgcca gttcaacttg ctggtatctg gcacgccagt
    33961 tgcctgacat caacattcag gtcttcacca atagccatcc gatttgccat gaactcggta
    34021 aacgcgaacg cattcaactg atcagttccg gcggcacact tgagcgcaaa tatggctgtt
    34081 acgtcaatcc ctcgctgatt tcccaactta aatcgctgga aatcgatctg tttatttttt
    34141 cttgtgaagg gatcgatagc agcggcgcac tgtgggactc caatgccatc aacgctgatt
    34201 acaaatcgat gctattaaaa cgtgccgcgc aatcgttgtt attgattgat aaaagtaaat
    34261 ttaatcgttc aggggaagcc cgcatcgggc atctggatga ggtaacgcac attatttctg
    34321 atgagcgcca ggttgcaact tctttggtaa cagcctgacg gaaagggtag caggccggaa
    34381 acgacgcccc ggccttgcct gttatcgctc gtcgcgacga ccacccaccg cagcccagat
    34441 gcggcggacg tgcaccgtca cttcttcgcg atcgtgatac aactgccgtg cctgaatctg
    34501 agcatttatg ccatgttcat caagctgtgc ctgaatatac gccagattgt gtgacacttc
    34561 ttcgtagcgt tttttcatcg gcagtttgag gttgaaaatg gtttcacggc accagccatt
    34621 aaccagccac tgcgccatca atgccgcaac tttcgccggt ttttcaacca tatcgcatac
    34681 catccaggag atattgctgc gcgtcggacg gaatttgaaa ccgtcttccc gcagccacgt
    34741 cacctgtccg gtatccatca gactttgcgc catcgggccg ttgtcgacgg aataaaccca
    34801 catgttgcgc ttcaccagtt ggtaggtcca gccgccaggg caagcgccta aatccaccgc
    34861 ccacatcccg ttcgccaggc gttcatccca ctcatccgca ggaataaaca catgaaatgc
    34921 ctcttccagt ttgagcgtgg aacgactcgg cgcatctgcc ggaaatttca ggcgcggaat
    34981 gcccatatag aacggcgaat tattgttgct gtatgagtaa ccggtatagc agcagcctgg
    35041 tgcaataaag aatacatgca caaccggacg cttcggcgtt tcatagttcg ccagcacccc
    35101 agcatcgcgc aaggcagcgc gtagcggaac agtaaattta cggcagaatt tcagcaactc
    35161 tttgctttcg ttggtatcgg caacttcaac acgcagttca ccgcccttct ctactacgcc
    35221 ctgcaacatg tcgacaatgg gggtaatacg atcttctggc ggcaaatgct gcaagagttc
    35281 ccccaccaca aaccactggc gggcaaaaat taatgaactg aacggcagct cacggattaa
    35341 cttatcgcca tcatcaggtt gataacattc ataaatgaca taacccgcat tctctttcac
    35401 gcgggcaaaa ccgaaaattt cccgctggcc ggctttatcg gtaatttctg cggcgcactc
    35461 tttttcaaag cccggacggc acagcaatac aaccttattc atgacttacg cccttgcgct
    35521 ttaaacggat agcaccaact aacattaacg cccagcccgc gaggaagctc acgccgccaa
    35581 ccggtgtgac aaatgcccac aaacgcagat gggacagcgc caggcaataa aggctgccgc
    35641 tgaacaacac cgtgcctaac gcgaggaaaa cgctactcca gtaaaaccag atactgatgc
    35701 gacgctgcat tgccaccgcc agacctaaga tcgccagcgt atgaaacgcc tggtattcga
    35761 ggccggtctg gatccagccc atctcaacgg cccccatggt tttacttaac acatgcgcgc
    35821 caaaagcgcc cagagccaca aaaatgaagc cgctaatggc ggcgaaaatc agcataaaac
    35881 ggctggtcat ggtcgtaccc tacgtaaatt attgttcata acgaaagcgg aatttttctt
    35941 gttcagcagc ggctttcgcc aggatccatt ggcgaaaggc ggctatttta cccagttctg
    36001 cctggctgtc atgacaaacc agataaaaag cgtttttact gaccagaaca tcattaaacg
    36061 ggcaaacaag acgtccggcc tcgatttcag attgcgccat cacgttattt gccagcgcca
    36121 ctccctgccc gtgaatagcc gcttgcagca ccatggcgct atgactaaaa attggccctt
    36181 gctgaacgtt gatatgattt aaccccaact gtcgggtata tgtctgccag tcacggcgtg
    36241 acgcatcatg taataacgta tgtttagcca gatcttccgg ggtcttcaag ggtttttcgc
    36301 ctgtcagcag tagcggcgaa cacaccggca ataaatattc ggcgtacagt ttttccaccc
    36361 gtagccccgg ccagttgccc cgaccataaa atatcgccac atcaacatca tccgccagct
    36421 tatcttcctg acgatcaacc gcctggattc gaacgtcaat tcccggataa gctgaattaa
    36481 agctggaaag tcgcggaacc aaccaatgaa tggcgaaact ggggagtaaa ctgaccgtca
    36541 acgccccctt ggcgctacgg gcctggagtt tacgcgtcgc ttcggttaat tgcgaaaata
    36601 tctctttgat atcgaggaaa tagctttgcc cttcctcggt cagcaggagt gaacgattac
    36661 ggcggcggaa cagttttagc cccagaaaat cctcaagaga cttgatttga tgacttactg
    36721 cggcctgggt cacaaaaagc tcttctgctg cgcgagtgaa acttaaatgg cgtgctgcgg
    36781 catcaaaaac tcgtaaggca tttagcggtg gtaatcgttt agacatggct tttaaacttt
    36841 gatgttaaat g
//
LOCUS       DAZKHC020000041        35385 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZKHC020000041 DAZKHC020000000
VERSION     DAZKHC020000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.041"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(183..914)
                     /gene="rlmB"
                     /locus_tag="ACHOSM_RS11725"
                     /old_locus_tag="pgaptmp_002345"
     CDS             complement(183..914)
                     /gene="rlmB"
                     /locus_tag="ACHOSM_RS11725"
                     /old_locus_tag="pgaptmp_002345"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGU4558856.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1094..3535)
                     /gene="rnr"
                     /locus_tag="ACHOSM_RS11730"
                     /old_locus_tag="pgaptmp_002346"
     CDS             complement(1094..3535)
                     /gene="rnr"
                     /locus_tag="ACHOSM_RS11730"
                     /old_locus_tag="pgaptmp_002346"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGU4558857.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3574..3999)
                     /gene="nsrR"
                     /locus_tag="ACHOSM_RS11735"
                     /old_locus_tag="pgaptmp_002347"
     CDS             complement(3574..3999)
                     /gene="nsrR"
                     /locus_tag="ACHOSM_RS11735"
                     /old_locus_tag="pgaptmp_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HGU4558858.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4204..5502)
                     /gene="purA"
                     /locus_tag="ACHOSM_RS11740"
                     /old_locus_tag="pgaptmp_002348"
     CDS             complement(4204..5502)
                     /gene="purA"
                     /locus_tag="ACHOSM_RS11740"
                     /old_locus_tag="pgaptmp_002348"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGU4558859.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5606..5803)
                     /gene="yjeT"
                     /locus_tag="ACHOSM_RS11745"
                     /old_locus_tag="pgaptmp_002349"
     CDS             complement(5606..5803)
                     /gene="yjeT"
                     /locus_tag="ACHOSM_RS11745"
                     /old_locus_tag="pgaptmp_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HGU4558860.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5885..6889)
                     /gene="hflC"
                     /locus_tag="ACHOSM_RS11750"
                     /old_locus_tag="pgaptmp_002350"
     CDS             complement(5885..6889)
                     /gene="hflC"
                     /locus_tag="ACHOSM_RS11750"
                     /old_locus_tag="pgaptmp_002350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HGU4558861.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6892..8151)
                     /gene="hflK"
                     /locus_tag="ACHOSM_RS11755"
                     /old_locus_tag="pgaptmp_002351"
     CDS             complement(6892..8151)
                     /gene="hflK"
                     /locus_tag="ACHOSM_RS11755"
                     /old_locus_tag="pgaptmp_002351"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HGU4558862.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8237..9517)
                     /gene="hflX"
                     /locus_tag="ACHOSM_RS11760"
                     /old_locus_tag="pgaptmp_002352"
     CDS             complement(8237..9517)
                     /gene="hflX"
                     /locus_tag="ACHOSM_RS11760"
                     /old_locus_tag="pgaptmp_002352"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HGU4558863.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9593..9901)
                     /gene="hfq"
                     /locus_tag="ACHOSM_RS11765"
                     /old_locus_tag="pgaptmp_002353"
     CDS             complement(9593..9901)
                     /gene="hfq"
                     /locus_tag="ACHOSM_RS11765"
                     /old_locus_tag="pgaptmp_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HGU4558864.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9987..10937)
                     /gene="miaA"
                     /locus_tag="ACHOSM_RS11770"
                     /old_locus_tag="pgaptmp_002354"
     CDS             complement(9987..10937)
                     /gene="miaA"
                     /locus_tag="ACHOSM_RS11770"
                     /old_locus_tag="pgaptmp_002354"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGU4558865.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10930..12777)
                     /gene="mutL"
                     /locus_tag="ACHOSM_RS11775"
                     /old_locus_tag="pgaptmp_002355"
     CDS             complement(10930..12777)
                     /gene="mutL"
                     /locus_tag="ACHOSM_RS11775"
                     /old_locus_tag="pgaptmp_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGU4558866.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12787..14121)
                     /gene="amiB"
                     /locus_tag="ACHOSM_RS11780"
                     /old_locus_tag="pgaptmp_002356"
     CDS             complement(12787..14121)
                     /gene="amiB"
                     /locus_tag="ACHOSM_RS11780"
                     /old_locus_tag="pgaptmp_002356"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HGU4558867.1"
                     /translation="MMYRIRNWLVATLLLCTPVGAATLSDIQVSNGNQQARITLSFIG
                     DPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDLT
                     ENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNPF
                     KTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVT
                     IAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRS
                     ATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFG
                     HSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEER
                     LLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14140..14601)
                     /gene="tsaE"
                     /locus_tag="ACHOSM_RS11785"
                     /old_locus_tag="pgaptmp_002357"
     CDS             complement(14140..14601)
                     /gene="tsaE"
                     /locus_tag="ACHOSM_RS11785"
                     /old_locus_tag="pgaptmp_002357"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGU4558868.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14573..16120)
                     /gene="nnr"
                     /locus_tag="ACHOSM_RS11790"
                     /old_locus_tag="pgaptmp_002358"
     CDS             complement(14573..16120)
                     /gene="nnr"
                     /locus_tag="ACHOSM_RS11790"
                     /old_locus_tag="pgaptmp_002358"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HGU4558869.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16119..17258
                     /gene="queG"
                     /locus_tag="ACHOSM_RS11795"
                     /old_locus_tag="pgaptmp_002359"
     CDS             16119..17258
                     /gene="queG"
                     /locus_tag="ACHOSM_RS11795"
                     /old_locus_tag="pgaptmp_002359"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HGU4558870.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEECGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17241..17294)
                     /locus_tag="ACHOSM_RS11800"
                     /old_locus_tag="pgaptmp_002360"
     CDS             complement(17241..17294)
                     /locus_tag="ACHOSM_RS11800"
                     /old_locus_tag="pgaptmp_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4558871.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17528..17603)
                     /locus_tag="ACHOSM_RS11805"
                     /old_locus_tag="pgaptmp_002361"
     tRNA            complement(17528..17603)
                     /locus_tag="ACHOSM_RS11805"
                     /old_locus_tag="pgaptmp_002361"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17568..17570),aa:Gly,seq:gcc)
     gene            complement(17639..17714)
                     /locus_tag="ACHOSM_RS11810"
                     /old_locus_tag="pgaptmp_002362"
     tRNA            complement(17639..17714)
                     /locus_tag="ACHOSM_RS11810"
                     /old_locus_tag="pgaptmp_002362"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17679..17681),aa:Gly,seq:gcc)
     gene            complement(17872..17947)
                     /locus_tag="ACHOSM_RS11815"
                     /old_locus_tag="pgaptmp_002363"
     tRNA            complement(17872..17947)
                     /locus_tag="ACHOSM_RS11815"
                     /old_locus_tag="pgaptmp_002363"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17912..17914),aa:Gly,seq:gcc)
     gene            complement(18160..18705)
                     /gene="orn"
                     /locus_tag="ACHOSM_RS11820"
                     /old_locus_tag="pgaptmp_002364"
     CDS             complement(18160..18705)
                     /gene="orn"
                     /locus_tag="ACHOSM_RS11820"
                     /old_locus_tag="pgaptmp_002364"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HGU4558872.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18800..19852
                     /gene="rsgA"
                     /locus_tag="ACHOSM_RS11825"
                     /old_locus_tag="pgaptmp_002365"
     CDS             18800..19852
                     /gene="rsgA"
                     /locus_tag="ACHOSM_RS11825"
                     /old_locus_tag="pgaptmp_002365"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HGU4558873.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19949..20917
                     /gene="asd"
                     /locus_tag="ACHOSM_RS11830"
                     /old_locus_tag="pgaptmp_002366"
     CDS             19949..20917
                     /gene="asd"
                     /locus_tag="ACHOSM_RS11830"
                     /old_locus_tag="pgaptmp_002366"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HGU4558874.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAAGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20939..24262
                     /gene="mscM"
                     /locus_tag="ACHOSM_RS11835"
                     /old_locus_tag="pgaptmp_002367"
     CDS             20939..24262
                     /gene="mscM"
                     /locus_tag="ACHOSM_RS11835"
                     /old_locus_tag="pgaptmp_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HGU4558875.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADAQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WIVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24413..25915)
                     /gene="yjeM"
                     /locus_tag="ACHOSM_RS11840"
                     /old_locus_tag="pgaptmp_002368"
     CDS             complement(24413..25915)
                     /gene="yjeM"
                     /locus_tag="ACHOSM_RS11840"
                     /old_locus_tag="pgaptmp_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HGU4558876.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(26134..27111)
                     /gene="epmA"
                     /locus_tag="ACHOSM_RS11845"
                     /old_locus_tag="pgaptmp_002369"
     CDS             complement(26134..27111)
                     /gene="epmA"
                     /locus_tag="ACHOSM_RS11845"
                     /old_locus_tag="pgaptmp_002369"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HGU4558877.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27436..29244
                     /gene="frdA"
                     /locus_tag="ACHOSM_RS11850"
                     /old_locus_tag="pgaptmp_002370"
     CDS             27436..29244
                     /gene="frdA"
                     /locus_tag="ACHOSM_RS11850"
                     /old_locus_tag="pgaptmp_002370"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGU4558878.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29237..29971
                     /gene="frdB"
                     /locus_tag="ACHOSM_RS11855"
                     /old_locus_tag="pgaptmp_002371"
     CDS             29237..29971
                     /gene="frdB"
                     /locus_tag="ACHOSM_RS11855"
                     /old_locus_tag="pgaptmp_002371"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGU4558879.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDQGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            29982..30377
                     /gene="frdC"
                     /locus_tag="ACHOSM_RS11860"
                     /old_locus_tag="pgaptmp_002372"
     CDS             29982..30377
                     /gene="frdC"
                     /locus_tag="ACHOSM_RS11860"
                     /old_locus_tag="pgaptmp_002372"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGU4558880.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30388..30747
                     /gene="frdD"
                     /locus_tag="ACHOSM_RS11865"
                     /old_locus_tag="pgaptmp_002373"
     CDS             30388..30747
                     /gene="frdD"
                     /locus_tag="ACHOSM_RS11865"
                     /old_locus_tag="pgaptmp_002373"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU4558881.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            30810..31943
                     /gene="ampC"
                     /locus_tag="ACHOSM_RS11870"
                     /old_locus_tag="pgaptmp_002374"
     CDS             30810..31943
                     /gene="ampC"
                     /locus_tag="ACHOSM_RS11870"
                     /old_locus_tag="pgaptmp_002374"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU4558882.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            32032..32565
                     /gene="blc"
                     /locus_tag="ACHOSM_RS11875"
                     /old_locus_tag="pgaptmp_002375"
     CDS             32032..32565
                     /gene="blc"
                     /locus_tag="ACHOSM_RS11875"
                     /old_locus_tag="pgaptmp_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU4558883.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32562..32879)
                     /gene="sugE"
                     /locus_tag="ACHOSM_RS11880"
                     /old_locus_tag="pgaptmp_002376"
     CDS             complement(32562..32879)
                     /gene="sugE"
                     /locus_tag="ACHOSM_RS11880"
                     /old_locus_tag="pgaptmp_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU4558884.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(33061..33207)
                     /gene="ecnB"
                     /locus_tag="ACHOSM_RS11885"
                     /old_locus_tag="pgaptmp_002377"
     CDS             complement(33061..33207)
                     /gene="ecnB"
                     /locus_tag="ACHOSM_RS11885"
                     /old_locus_tag="pgaptmp_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGU4558885.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33318..33443)
                     /gene="ecnA"
                     /locus_tag="ACHOSM_RS11890"
                     /old_locus_tag="pgaptmp_002378"
     CDS             complement(33318..33443)
                     /gene="ecnA"
                     /locus_tag="ACHOSM_RS11890"
                     /old_locus_tag="pgaptmp_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGU4558886.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33495..34061)
                     /gene="efp"
                     /locus_tag="ACHOSM_RS11895"
                     /old_locus_tag="pgaptmp_002379"
     CDS             complement(33495..34061)
                     /gene="efp"
                     /locus_tag="ACHOSM_RS11895"
                     /old_locus_tag="pgaptmp_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGU4558887.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            34103..35131
                     /gene="epmB"
                     /locus_tag="ACHOSM_RS11900"
                     /old_locus_tag="pgaptmp_002380"
     CDS             34103..35131
                     /gene="epmB"
                     /locus_tag="ACHOSM_RS11900"
                     /old_locus_tag="pgaptmp_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGU4558888.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8427 a         9686 c         8970 g         8302 t
ORIGIN      
        1 ttttgttcag gtacagtttg cagcgccgtc gccagcgcca acggattcca tgtcatatat
       61 tattccatat agatttagtt taaatattaa taaaatgaat atttgcagta cgtaattatc
      121 ttaccagcta tagacaaaaa aaaaccatcc aaatctggat ggcttttcat aattcagacg
      181 aactagctgc gctgacgaac cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc
      241 agggaagaaa cacttcctgc cattgggatg ctgatcaact catcgcaatg ttcacgggtc
      301 aggcgacgca taccttcacc ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg
      361 ctctgataga gtgtatgatc cgcctcgcct gccgtaccga cgatccagat attctcttcc
      421 tgcaacatac gcatggtgcg cgcaaggtta gtcacccgaa tcagtggaac gctttctgcc
      481 gcgccgcagg ctactttttt cgccgtagcg ttgagctgtg cggagcgatc tttcggcaca
      541 atcaccgcgt gaacgccagc agcgtccgcg ctacgcaggc acgcgccgag gttgtgcgga
      601 tcggttacac cgtcgaggat cagcaggaac ggttgatcga gcgaagcgat cagatccggc
      661 agatcgtttt cctgatactg acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg
      721 gcaccgtcgc ttttctcgtc gagatattgg cggtttgcca actggataac cacgccctga
      781 gactcaaggg cgtgaatcag cggtaacaga cgtttatctt cacggccttt taaaataaag
      841 acttcctgaa aacgttcagg ggcgcgctcc agcagggcct gcactgcgtg gatgccgtaa
      901 atcatttcgc tcattaatgt actcgttgtt tacgttttgc ctgatgcact acgtttatcc
      961 ggcctacatg atctctgcaa tatattgaat ttgcgtgatt atgtaggccg gataaggcgt
     1021 tcacgccgca tccggcatga acaaagcgca ttttgtcagc aatctaaccc tcttctttta
     1081 aagagggtat tgatcactct gccacttttt tcttcgccgc acgcttcgct ttggtcgctg
     1141 cggctatttt ctgcgttttc gccgatggct ttttcgcttt tcttgcgtct ttcttcgccg
     1201 ctttcggctt cgtttttttc tcaccgcgga aggcgctgtc tggctcaaag tttacctttt
     1261 taccgacctg acgacgcttg ccgccttttt tgcctgcatc gccttttttc gctttctcgc
     1321 gcgccgtttt accgacgttg cgcggtgcgc gttcactgga gatcagacta aagtcgattt
     1381 tgcgctcgtc catattaacc gcttcgacgc gaacttccac gcgatcgccc aggcgataag
     1441 tctggccgct ggattccccc atcaggcgtt gccctacctg gtcaaagcga tagtagtcat
     1501 tgtccagcga agagacatgg accagaccat caatgaacaa gtcgtccaga cggacgaaga
     1561 agccaaagcc agtgacgctg gaaattacgc ctttaaagac gttacctacc tggtcgagca
     1621 tgaagtcaca cttcagccag tcagcgacat cgcgcgttgc ttcgtcggca cgacgttccg
     1681 ccatcgaaca gtgctgaccc agttgcaaca tctcttccat cgaataatgg tagccgccgg
     1741 tttcagtggt gttgccctga tgcccctgct ctttcgccag cagatattta atggcgcggt
     1801 gcagcgtcag gtcaggataa cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg
     1861 ccagaccgaa gtgaccacgg ttttctggat cgtaaatcgc ctgtttcatc gagcgtagca
     1921 gcatggtttg cagcatttct gcatcaggac ggtcggcaac cgactccagc agttccgcgt
     1981 agtcacgcgg ttccggcttg ttaccacccg gcagctccag ccccagctcc gccagcactg
     2041 aacggaaaga ggtaatcgct tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt
     2101 ctttcgcttt ctcaacgaaa cgcgccgccg agatattcgc cagaatcatg cactcttcaa
     2161 ttaatttgtg cgcgtcgtta cgctgggtct gttcgatacg ttcaatacgg cgttcagcgt
     2221 tgaaaatgaa cttcgcttct tcgctctcaa atgagatccc accgcgttct tcacgggctt
     2281 tatccagcac tttatagagg ttatgcaact cttcgagatg cttaaccagc ggggcgtact
     2341 gctcgcgcag atcctgatcg ccctgcagaa tatgccagac tttggtgtag gtcagacgcg
     2401 cgtgagagct catcaccgct tcgtagaatt tgtagcccgt caggcggcct ttcgacgaaa
     2461 ccgtcatctc gcacaccata cacaggcggt ctacctgcgg gttgagcgaa cacaggccgt
     2521 tagagagcac ttccggcagc atcgggataa cctgcgaagg gaagtacacc gacgtgccac
     2581 ggttacgcgc ttctctgtcc agcggcgttg gcggacgcac atagtagctg acgtcggcaa
     2641 tcgcgaccca taaacgccag ccgccgccgc gttttttctc gcagtaaact gcatcgtcaa
     2701 agtcacgggc gtcttcgcca tcaatggtga ccagcggtaa atcgcgcaaa tcgacacggc
     2761 ccgcttttgc ttcttccggc acttcttctt tcagcccggc aacctgttgc tcaacagcct
     2821 gcggccagat atacggaatt tcatgggtac gcagagcgat atcaaccgcc atgccggtgc
     2881 ccatattgtc gcccagcact tcgacgattt tacccaccgc tttggtgcgg cgagtcggac
     2941 gctgagtcag ttcgactacg accacaaagc ccatccgcgc gcccatgatc tgatcgggcg
     3001 ggattaagat atcgaagctc agacggctat cgtcaggaac cacaaagccg acgcccgctt
     3061 cggtaaagta gcgaccaaca atctggctgg tttttggcac cagtacgcgg acaatacgcg
     3121 cttcacgacg acctttacgg tcagcaccca gcggctgcgc cagcacctga tcgccatgaa
     3181 tgcaggtttt catctgctcg ctggagagat acaaatcatc tttacgccct tcaacccgca
     3241 gaaagccgta gccatcacgg tggccaataa cggtaccttt caccaggtcg aggcgttccg
     3301 gcagcgcata gcactgacga cgagtgaaga ccagttgacc atcgcgctcc atcgcgcgca
     3361 ggcggcgacg caggccttca agctgctctt cgccttcaat gtgcagttct accgccagct
     3421 catcacggct ggccggtttt tcacgtttgg ttaaatgttc gaggataaat tcccgactag
     3481 ggatgggatt cgcgtatttt tcagcttcgc gttcctggaa aggatcttgt gacatctcgg
     3541 ttcctccgtt gtcatctctg atgaagattt tcgtcactcc accagcaata atttataaag
     3601 cggttgattc tcttcaacca aatcggcaag cgtgtagtta tccagttccg taagaaaact
     3661 ttgcacggcc ttagaaagcg cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc
     3721 actgctgcaa ttcaccagcg ataagggctc cagctcgcgc accacatcac caatacgtat
     3781 cgcactcgcc ggtttaccca ggcgaatgcc gccatttttt ccacgaacag cagtcacgta
     3841 gccggcacga ctaagttgat tgattatttt gaccatatga ttacgggaga cgccgtagac
     3901 gtcagtcact tcagaaatac tggtcatccg cccttctggc aatgacgcca tgtagatcag
     3961 cgcacgtaat ccgtaatcag tgaaactcgt taactgcaca tcaacctcaa aagggaaatc
     4021 gggaaaaaat acatttatat tgatgataaa ccagccacaa gctgtgtcgc taatttattt
     4081 cagttcgggg aaggaaaaaa gcgaggattt aacactgtgt cggatagcgt aatggcttaa
     4141 ccgacacagc aatgtatcac actgttagcc cggcaggcaa aatatctgcc aggcgtacca
     4201 gaattacgcg tcgaacgggt cgcgcagaat catggtttca gtacgatccg gaccggtaga
     4261 gatgatatcg atcggcacac cggtcagctc ttcaatacgc ttgatgtagt tcagcgccgc
     4321 ctgcggcagg ccgctacgat ctttcacgcc gaaagtggat tcagaccagc ccggcatggt
     4381 ttcgtaaatc ggctctacac ctttccagtc gtcagctgcc agcggagtag tagtcacttc
     4441 acgaccatcc ggcatacggt aagccacgca gagtttcacc tctttcaggc catccagaac
     4501 gtccagttta gtcaggcaga agccagacag ggagttcaac tgtaccgcac gacgaacggc
     4561 aacggtgtcc agccagccgg tacgacgacg acgacccgta gttgcgccga attcgttacc
     4621 ttgcttgcag aggaactcgc cagtttcatc aaacagttca gtcgggaacg gacctgcacc
     4681 cacacgagtg gagtaagctt tgaggatacc cagaacgtaa tcaacataac gcgggcccag
     4741 gccggaaccg gtcgccacgc caccagcagt ggtgttggaa gaagttacgt acggataagt
     4801 accgtggtcg atatccagca gtgtaccctg cgcaccttca aacatgacga aatcgccacg
     4861 ctgacgcgcc tggtcgagca ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc
     4921 aacagccatc gtatcatcca gaactttctg gtaatcaacc gcttcagctt tgtagtagtt
     4981 aaccaactgg aagttgtgat attccatcac ttctttcagt ttttcagcga aggtttcttt
     5041 gtcgaaaagg tcgccaacac gcagaccgcg acgtgccact ttatcttcat aagcaggccc
     5101 gataccacga ccggtagtgc cgatcgcttt cgcgccacgc gctttctcac gtgcgttatc
     5161 cagcgcaacg tgataatcaa ggatcagcgg acatgcttca gacagcagca gacgctcacg
     5221 aacggggatg ccacggtctt ccagttcttt catctctttc atcagcgcgg ccggagacag
     5281 cacaacaccg ttaccgatga tgctggttac attctcgcgg agaatacctg atggaataag
     5341 atggagaacg gttttttcac cgttgattac gagagtatgg cctgcgttgt gaccgccctg
     5401 gtagcgtaca acatatttag cccgttcagt cagaagatcg acgatcttac ctttaccttc
     5461 gtcaccccat tgggtgccca gtacgacgac gttgttaccc atttttcaaa atcaccgttt
     5521 gcttaaaaat ggattctacc atcgcttttt cagagttaca gcactttttg cactcaaaaa
     5581 tgacctcgat cagtctgttt tttgttcagc caatcgtttt cctcaacatg tagtagacca
     5641 caacgcccgc aaccacaagt ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg
     5701 tcatcgcaga gatcatcttc ttccatgcct tcgggtaaag catcggccct aaaccttcca
     5761 gtaccaaaac cagggcaagc gccagccaga ttgtcgaatt cattcattat ccttatagaa
     5821 aaagaaaacc accgacatcc ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt
     5881 tatattaacg cgttgcggaa gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc
     5941 tcatgaccat cacgtcctga ttgccagaga agctgttctc ataagcacgc aggctacgga
     6001 tgaatgcgta gaagtccgga tctttactga atgcatcagc aaacagtttg gctgcttcgg
     6061 catcgccttc accacgcata atgcggccct gacgctcagc ttctgccagc gttctggtca
     6121 cttcatagtc ggcagtcgcg cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac
     6181 gacgcgctac cgcttcacgc tcggcgcgca tacggttgta gatcgcttca gacacttcgg
     6241 tcggcaggtt gatctgcttg atacgcacat cgacaacttc aatacccagc gccgccatac
     6301 tgttcgggtt gatgaccgga actttgccct tcgtctcagc cgttacgcgc tctgccgctt
     6361 cggcaatggc gttatctgct gccggggtag taacttcatc ttctgtaccc gcagaaccgg
     6421 agttcagcgc gtcacgtact tcgagggtca gacgaccacg ggaatcggtg acgatatctt
     6481 tcacgtccag gcgaccaatt tcagaacgca gacggtcaga gaacttacgt ttcaacagca
     6541 cttccgcttg cgaaatgtcg ccaccacccg ttgccaggta gtaacggctg aaatcgctga
     6601 tgcgccattt gatgtaagag tcgacgatca ggtctttctt ctctttggtc acaaagcggt
     6661 cggcctggtt gtccatggtc tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg
     6721 gtatcttgaa atgcagaccc ggctcataaa ccagaggttt gttgtcatcg tcacgcagta
     6781 ccttaccaaa acgcagcgta ataccgcgct caccttcttt gacgacaaag acagacatgt
     6841 aaagcactac cagcacgatg atgataatcg cgataactga cttacgcatc gttattcccc
     6901 ctgacgctgg tagtcgttac gctgcgcgtt ggcgcggcgt tggtccataa tatcgccctg
     6961 actggtggac gacgtgttgc ttgctccact ggttgtggag gaagaggctg gcggcaaacg
     7021 cagcagattg ctggcaccgt tatcgctctt cgccgcaggg gcgttaccac ctttcagcat
     7081 ctggtctaac ggcagaacca tcaggttgcc acctttatcg ttaaccagca ctttgcgggt
     7141 gttacccaac actttttcca tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc
     7201 ggctttatat tccggcagaa gtttagcaaa gcgcgccact tcaccctgag cttccaggat
     7261 ggtctgggcc ttgtacgcac gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg
     7321 cggctgaact tcgttggtat acgcttctgc ttcacgaatg tattgctgtt cgttttcacg
     7381 cgcggcaatc gcatcgtcaa acgccgcttt tacttcttcc ggcggacgag cagcctggaa
     7441 gttgacgtcc agcagcgtga tacccatgtc atacggacgg atagtctctt ccagttcgcg
     7501 ctgagtatcg ctacgaatca cggtacgacc ttccgtcaga atgcggtcca tggtgtattt
     7561 accgataacc ccgcgcaggg cgctgtcggt agcctgacgc agactgtcat ccgggctggt
     7621 cacgctatac agatattttt ccggattggt aacgcggtac tgcacgttca tctcaacgcg
     7681 cactacgttc tcgtccgacg tcaacatcac accggaagcg gccagttcac gcacggcttc
     7741 cacgtttacc ggtttgactt cgtcgataaa cgtcggtttc cagttcagac ccggctcaac
     7801 cagatggctg aatttaccaa agcgtgttac cacgccgcgt tcggcttctt taatggtata
     7861 gaaaccactg gccgcccaga taatgacaat cgctgccgct gcgatggtaa cgacacgacc
     7921 gccaagctgc gggcgcgggc cttgcgatga actgccaccg ccagatccgg tgcctttacc
     7981 gccgcccaga ccaccgagct ttttgctcag tttgcggaag atatcatcta aatcaggtgg
     8041 cccttgatcg cgaccgcctt tgtttccatt tccctcagag ttgccgccag gtttgctgct
     8101 tccccacggg tcgcggtctt gtccgttatt accgggctga ttccacgcca tgtttgtgct
     8161 ccatatttgt tatgcggtga tccctgttgt ctttcgacgc cagaggatat gactccacgc
     8221 ttcagacgct acgccgttag atcaggtaat cgatcaacgc cggttcttgt ttacagaggc
     8281 gacgccagtc aacgatcggc atacgaactt gcagacttac gctgccgtcc tcctccatcc
     8341 actctttttc tattgcctga agctgataaa aacgacttct cagacgtcct tcctgcggcg
     8401 gcaaacgcaa tgtatgctgc gccacctcgc cggaaagccg ctccgtcaaa gcctgaaaaa
     8461 gctgtggtat ccccgctccg gtctgtgcgg aaagccagac acggatcggt ttgttctctt
     8521 cgtcccgatc aatacgcggt tcgaaatctt ccagcatatc gatcttgttc atcaccagca
     8581 gggttgggat ctcgtgagcg tcgatctctt caagaaccgt attcaccgct tcgatgtttt
     8641 cttgtacacg tacatccgcc gcatcaatga cgtgcagcag taatgtggct tgccgcgtct
     8701 cttgtaacgt ggctttaaat gctgccacca gatcgtgcgg caggtggcga ataaacccta
     8761 cggtatctgc aagtacggtt tcaccgacat ccgcgacgtc aatacgccgc aacgtcgggt
     8821 cgagggtggc aaacaactgg tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa
     8881 gggtagattt accggcgttg gtatatccca ccagcgaaac agtaggaacg tcggctttga
     8941 tacgcgattg ccgcccctgc tcacgctgct tttcaactct ttccaggcgc gactgtatct
     9001 gcacgatgcg attacgcaac aaacgacggt cggtttcgag ctgggtttca cccggaccac
     9061 gcaaacctat cccgcctttc tgtctttcaa ggtgggtcca gccacgcacc aggcgcgtag
     9121 ccagatggcg cagctgcgcc agctcaacct gcaacttacc ctcatgggta cgcgcacgtt
     9181 gggcgaaaat atctaaaata aggccggtgc ggtcgataac acgacactcg cacaaacgct
     9241 ccaggttacg ctcttgcgcc gggctcaggg catggtcaaa aagaacgacc gaagcacccg
     9301 tcgctttgac agcttccgca atttcaactg ctttaccttc acctacaaaa tactttgggt
     9361 gcggcgcttt acggctaccg gtaatcacct gcaatgcttc gacaccggcg gaagagacca
     9421 gagattcaaa ctcctggagg tcttccatat ctttgtcttg cgtaaaatag atgtgtacca
     9481 gtaccgcctg ctcaccagca tcataacggt caaacaagcg tataaccctc taaatagatc
     9541 agcggggaac gcaggatcgc tggctccccg tgtaaaaaaa cagcccgaaa ccttattcgg
     9601 tttcttcgct gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tggtagttac
     9661 tgctggtacc gccaccggcg ttgttactgt gatgagaaac cgggcgagac gggacaacag
     9721 tagaaatcgc atgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact
     9781 gatcaaaaga ctcgatttgc ccttgcagct taataccatt caccaaataa atagaaactg
     9841 gaacacgttc ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca
     9901 ttctctcttt tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc
     9961 gcacgatacg tctcaattgt acacattcag cctgcgatag caccaacaac ctgtaatact
    10021 tcgtcacgcg cctgttctgg tttttcactg tcaagccagt gaaccccttc ccaaccacgc
    10081 agccaggtta tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt
    10141 tcatcgtatg agatttcgcc ttcaaggtaa gaccacatct ggcgataacc cacgcaacga
    10201 atggaaggca agtccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca
    10261 aaacctgaag ccaacatctg atgaaaacgc tgctcaatgc gttgatggag cagttcacgg
    10321 ctcgccgggg cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc
    10381 gtcagttccg ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt
    10441 tgtggatcat ttggatgaat ccttgctgcc gcaaccggat ctacctcctg aagttgacga
    10501 tgcaatgact cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg cacttccgga
    10561 tctgccgacg gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg
    10621 cccactaaca gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg
    10681 cggcgaaaat cagcagccga gtaagcctgc gacggatcgc gaatatccag caatcggtgc
    10741 ggcgcggcga gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa
    10801 ataagggcag aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct
    10861 aacgccgttt taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc
    10921 ttactgatat cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa
    10981 cagaccaccc ggcggcgttt tcacaagttg cggacataac cgttccacgt ccgccagcag
    11041 ggttatggcc tgtgccattg accactgcgc atgttcgctc atcagatttc gtgcaatcca
    11101 ctgcgcaata ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta tcagttcagg
    11161 aatcaagatt tgtaaatttt gttggcgtaa gggtaaaggc acggccctga tggtcacatg
    11221 ctgtgcatct gactggaaat caatacccaa ttccgccaag gcagactgcg ctttttctaa
    11281 tgccgatttt tcttcgccag aaacttttag ccgcaacgga ataagcagcg gctgagcgca
    11341 aactggcgct tcacccggcg tcagttgtac ctgacgcagc caacgttctg ccactggcaa
    11401 ggctaaaagt gaaatgttgc cgtcgcgctc cagcaacgca cagtcggaat ggacgatagt
    11461 cagtacccga ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc
    11521 ttttaatttt tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg
    11581 tttctggtag cctggctgcg cattcggcca gggggcagcc ggacgactgc ctgatgctgg
    11641 cgcaggagtg tagcgcggag ctaccggctc acgagctgcc ggttctgcaa agtgattgcg
    11701 ccccgccgcc acgcggtttt ccggaatgga acgcggtgca ggttggggtt catcgtccag
    11761 cggtagcggc gtttccagtt gctgttgtag cacgctcagc acgccctgat agataaaatc
    11821 atgcaccaga cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgca cgttgacgtc
    11881 cacctgatgc ggatcgatct ccagatacag cacaaatgcc ggttgctgat cggcccccag
    11941 tttgtcttcg caggcctggc ggatcgcgtg attgatcagg cgatcgcgca tcatgcgacc
    12001 gttcacgtag caatactgaa tttctgccag tgcgggcgtg gtgtgatttg gatcggccac
    12061 ccagccgcgt agcgtgagat cgccgtgttg ccattcaatc gccagcgctt gttcaagaaa
    12121 agcggtgccg caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg
    12181 gtactgacgc acaattttac cgttatgcga caggttgatc gtgacgtcga aacgcgccag
    12241 cgcaatgcgg cggatgatct catcaatgtg gttaaattcg gttttctcgg tgcgcaggaa
    12301 tttgcgccgc gccggggtgt tgtagaacag atccagcacc tccagcgtcg tccccacagg
    12361 atgcgccgcc ggttttaccg tcacgttcat atcgcgccct tcggcatagg cctgccaggc
    12421 ttcctgctgt tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc
    12481 cagcgcctca ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat
    12541 tttactggtg gcatgacgag ccagcgccag cgccagctca tcttttttga taccgcagcc
    12601 gttatcacga atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgcgt
    12661 cgcacctgca tcgaggctgt tttccaccag ttctttgact accgacgcag gtcgctcgac
    12721 cacctcacct gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa
    12781 tcgtccttag tttggcagcg tgcgatctgg cgttgtcacc gtactggccg tttgtgccgt
    12841 tgccccctgc ggcgcagatt gcatcggatg cgcaaggaaa taattgcgca ggcctttgta
    12901 aatggcttct gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt cttcactgtt
    12961 attgctgata aaaccggttt cgaccagtac tgacgggata tccggcgaac gcagaacgcc
    13021 tagactagcg tgttctggcc gacgtttatg aatttcgcca atgcgttgca actgactgat
    13081 catactggtc gctacatcat accctacccg ctgggaatga ccgaactgta aatccagcac
    13141 cgcctggctt aaataggggt cagactgact gttcgccagc acatcacccg ccccacccag
    13201 cagctccgac tgtttctcgt gctgctccag ccagctggcc atttcgctgt tggcgcgacg
    13261 gttagagagc acccatacgg aagcgccagt cgcactgcgg ttcggtgcgg catcagcgtg
    13321 aatcgacacg aggaaattgg cgttttgctt acgtgccaca tcactgcgcc ccatcaccga
    13381 gataaagtaa tccccgtcac gggttaaaac gcccttaaac atcggatcgt cattgagcaa
    13441 agtacgcaat ttacgcgcga tggcgatggt gacatttttc tcccgcgtac caccggggcc
    13501 gatagcgcca gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc
    13561 agtgttagcc gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga taacacccgt
    13621 agtgcggtta ctttccgttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac
    13681 aaccgcaggc gtttcaacgc gtttcgcaac cacgggcggc ggaggaggag gtggcggcgc
    13741 atcggcgtta atcgtaaaga cgacagtgta attgctgcca ttctgccgct tcaccgcttc
    13801 ggttttaccg ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt
    13861 tccagagcga atcgccttca ccagattatt gccgctgaac aacaacggca gtccctgaat
    13921 cacgcccgtt tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata
    13981 atcaggatcg ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg
    14041 aatatcagag agcgtcgcgg cacccaccgg cgtgcacagc agcagcgtcg ctaccaacca
    14101 atttctgatg cgatacatca tcccgccacc tttcaaaggt taaccggcta aacgcgccag
    14161 caacaattca cccgcagagg aaaccgcact cacgcgcgcc tcacggcctt gtgcctgata
    14221 atcaatgtgt atttcgacat ccgggtcagg aagaacacct gtaccttgtt gtggccactc
    14281 caccaggcag atggcatcgt tggcaaaata atcgcggatc cccataaact ccagctcctc
    14341 gggatcggca aggcggtaca aatcaaagtg ataaaccatt aaattgtcga gcgtataggg
    14401 ttcgaccagc gtataagtgg ggcttttgac attaccctga tgacccagag cctgtaaaaa
    14461 gccccggcta aaggtggttt tacctgcgcc taaatcgcca tacagataga ttacggttgc
    14521 gccatcgcag gctttcgcta cccgctcgcc caggtctaat gttgcctgct catcagggag
    14581 cggaattact cgattcatca tggtttttat cagtcacttc cgggttaaca atacgctgta
    14641 gcgtggaaaa gagatcggtt gccagcatcc cgcgcgttcc aaaacgcgcc gccagtacgt
    14701 cagctgccgc accgtgcgcg acacaacccg cacaggcggc atcatacggc gacagttttt
    14761 gcccaagcaa tgcgccaata ataccagaga gcacatcgcc catgccgccg ctcgccatgc
    14821 ctgcatttcc ggcatcaata atgcctaaag cgtcaggatg ggcggcgacc acggttccgg
    14881 cacctttcag caccgctacg ccgccataac gttgtaccag acgtttggcg caatgtaagc
    14941 ggtcactttc aatttcagcg acggaacagc ctaacaaccg tgcggcctcg ccaggatgcg
    15001 gcgtgatcac gcgattgtga cgcttatcgg gattgattgc cagcaggttc aatgcatcgg
    15061 catcccacaa catcggtttg cgaaaattct caactttttg cagtgctttt ttcccccact
    15121 cttgctggcc cagaccggga ccaatcacca ccacatcggc ccattccagg ctttcggtaa
    15181 gagagtccat cgtcagttca tgcaccatca attccggtcg tgcagtcagc agcggcgcaa
    15241 tgttttcact gcgggtcagt actcggacta aaccagcacc agcacgcagc gccgcttccc
    15301 ccgtcatacg aatagccccc gccgtgccgt gatcgccacc gataattacc agccgcccgt
    15361 gatcgccttt atgcgaagtc gggcgacgcg gttttagcca gtgagaaagt tgttctgctg
    15421 aaaaccgctg aattttcgtc tcctgacctg ccagccaact atccagcccc agtgagtcaa
    15481 aatgcagttg tccggtaaca tcccgcgctt ttccagtgag caagcctggt ttcagcgcaa
    15541 taaaagtgat ggtgtgatcg gcgttgatca ccgcgcctgg cgtagcgcca gtttcagcca
    15601 gcaggccgga agggatatca accgccacaa tcggcgcagg atgggaatta gcgtggtcga
    15661 ttaactggct aatggattcg cggggcgctt gccgcaaacc ggtaccgagc agcgcatcaa
    15721 caatcagatc taccgattcg ggccagacaa tattcgaagc atggatctcg ccacccgcgt
    15781 ttaaccatgc ttcgcgtgcc agcgcggcct cttccggcaa cggtttgtcg ctctcctggg
    15841 ccaacaacgt gacctcaatg ccgaccgctt tggccagtcg cgcgaccacg tagccatcgc
    15901 cgccgttatt accatgaccg cacagcacca gccagtggcg ggcgtcagga tacgccgaac
    15961 gacacacctg gaatgcggcc tcgccagcgc gaagcatcag ctcatagagt gtgagcccca
    16021 gcacatctgc cgcctcgcgt tctccgcggc ggatatcgtc ggcgtaccag acggtgtgtg
    16081 gtatacttac ggggtttttc ttcattgtat ggtccgtcat gtcagagccc ctcgatctca
    16141 atcagttagc gcaaaaaatt aaacagtggg ggctggaact gggctttcag caggtaggta
    16201 ttaccgatac cgatctcagc gagtccgagc ccaaactgca agcatggctg gacaaacaat
    16261 accacggcga aatggactgg atggcacgtc acggtatgct gcgcgctcgc cctcatgagt
    16321 tattgcccgg tacgctgcgc gtgatcagcg tgcggatgaa ttaccttcct gctaacgccg
    16381 catttgccag cacgctgaaa aaccccaaac tcggctatgt tagccgttat gcgctgggcc
    16441 gtgactatca caaacttctg cgcaaccgac tcaaaaagct gggcgagatg attcagcaac
    16501 attgtgtttc gctgaatttt agaccgtttg tcgattctgc gcctattctc gagcgcccgt
    16561 tagctgaaaa agctgggctc ggctggacag gtaagcactc acttatcctc aatcgcgagg
    16621 ccggttcgtt cttcttttta ggcgaattgc tggtcgatat tccgctgccc gtggatcaac
    16681 cagtcgagga agaatgcggc aaatgcgtgg cctgtatgac gatttgcccg accggtgcca
    16741 tcgtcgagcc atataccgtc gatgctcgcc gctgtatctc ttatctcacc atcgaacttg
    16801 aaggggcgat cccggaagag ttgcgaccgt taatgggaaa ccgtatttac ggttgcgatg
    16861 actgccagct tatctgcccg tggaatcgct attcacaact caccacagaa gaggatttca
    16921 gcccgcgtaa gccgctacac gcaccggaac tcattgagtt attcgcctgg agcgaagaga
    16981 agtttttaaa agtcacggaa ggatcggcga ttcgtcgtat tggtcacctg cgttggctgc
    17041 gtaatatcgc cgtagcatta ggcaatgccc cttgggatga aacgattttg acagcgctgg
    17101 aaagtcgtaa aggtgagcac ccacttctcg atgagcacat agcgtgggcg attgcgcagc
    17161 aaatagagag acgaaatgcg tgcatagtcg aagtgcaatt gccgaaaaaa cagcgtctgg
    17221 ttcgggtgat tgaaaaaggg ttaccgcgtg acgcctgatt catccacagc ttgtgaataa
    17281 aaataaaaac gcattgcaat tcaagacgaa taaaatcgtc aagtgatcga ctcgacaaaa
    17341 cggaatctta atttttaact tatctatcaa taggttaaaa aaatactatt caccgtgcga
    17401 agtttctttg aaattaagat tacagcctga ggctgtggat aactctgttt acaagagttt
    17461 ttcagagaca acgaaaacca gcaataacgc atcgcatcgc tgtggatatt ttattgagag
    17521 aagaatttgg agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc
    17581 tctaccaact gagctattcc cgcttgggta gtctgtgcct tacagcactt tcaaattttg
    17641 gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg ctctaccaac
    17701 tgagctattc ccgcttaatc ttcgtctttc gaaccgctac tgccttggct gccttcactc
    17761 accttagtca catacttttg taagttccca aggattcgtt cagttgccgc cttgtcgcaa
    17821 ttcgaaatac tgcgattggg tggtctgtgc tttgcagcac ttttcaaatt ttggagcggg
    17881 aaacgagact cgaactcgcg accccgacct tggcaaggtc gtgctctacc aactgagcta
    17941 ttcccgcgta ctacttaatt ttgctttcgt aattttcgca tttctgcgtc gttacgggag
    18001 gcgcattata cgagaaatct ttccctctgc aacccccccc aaaaaagttt tttttgcgtt
    18061 tttatctcaa gtgctgaaat tatcagcaag acgttcaatt tagcgtcaaa atcacgccgc
    18121 cgcaagtgga ttccggcgct tacgtgacca ggaaaaattt tacagcttga taaaatgctc
    18181 gcggtagtaa gccagctccg ccaccgattc acggatatca tccatcgcct gatgcgtccc
    18241 ctgcttggta aaaccatcca gaatttccgg cttccagcgg cgcgccagct ctttcagggt
    18301 gctgacatcg agataacggt agtggaagta ggcttccagc tccggcatgt atttaaacag
    18361 gaaacgacgg tcctgtccga tgctgttacc gcaaatcggc gattttcccg caggcaccca
    18421 ctgttttaag aattcgagcg ttgccagttc agcttcccga tcgcccatcg tgctcgcttt
    18481 cacgcgctct accagcccgc tggcggtatg ggtgcgcacg ttccagtcat ccatcagcgc
    18541 cagctgttca tcggactgat gtactgcaat ggtcggccct tctgccagaa tattcagatt
    18601 ggcatcggtc accagcgtgg caatctcaat aatgcgatcg cgctcgggat ccagaccggt
    18661 catctcaaga tcgatccaaa tcaggttgtt ttcattggca ctcatgctat tttccaccca
    18721 ttcacagtga ctatattgat ccacaattgc gtgtatcata gatgttttgc ccatcagggg
    18781 cgaccaggag tcagtacgat tgagtaaaaa taaactctcc aaaggccagc agcgccgcgt
    18841 gaacgccaat caccagcgtc gtcttaaaac gtctaaggag aagcccgact acgacgacaa
    18901 tctgtttggc gagcctgatg aaggtatcgt catcagccgc tttggtatgc acgctgatgt
    18961 ggaatccgcc gatggcgacg ttcaccgctg caatattcgc cgtaccatcc gttcgctggt
    19021 aaccggcgac cgcgtagtct ggcgtccggg taaaccggcg gcggaaggcg taaatgtcaa
    19081 agggatcgtg gaagcggtgc atgagcgtac ctcggtgttg acgcgtccgg atttctacga
    19141 cggcgtaaaa cctattgccg ccaacatcga ccagattgtc attgtctccg ccattttgcc
    19201 ggagctgtcg ctcaatatta tcgaccgtta cctagtggcc tgcgaaacct tgcagattga
    19261 gccgattatt gtgctcaaca agatagacct gctggacgac gaaggcatgg cgttcgtcaa
    19321 cgagcagatg gatatctacc gcaatatcgg ttatcgcgta ttgatggttt ccagccatac
    19381 tcaggatggg ctaaaaccgc tggaagaggc gttgaccggg cgcatcagca tttttgccgg
    19441 gcagtctggc gtcggcaaat ccagcctgct gaacgcgttg ctggggctgc aaaaagagat
    19501 cctgaccaac gatgtctctg acaactcggg gcttggtcag cacaccacga ccgccgctcg
    19561 gctgtatcac ttcccgcacg gcggtgatgt gattgattcc cctggtgtgc gtgagttcgg
    19621 cctctggcac ctggagccgg aacaaatcac tcagggcttt gtcgaattcc atgactattt
    19681 aggtctgtgt aaatatcgcg attgcaaaca cgataccgat ccgggctgcg ctatccggga
    19741 agcggttgag gaagggaaaa tcgcggaaac ccgtttcgaa aactatcacc gtattctgga
    19801 aagcatggcg caggtaaaaa cgcgtaaaaa cttttctgat acggatgact gacaattaag
    19861 ctaaccgtcg ttagaatcgt cccctttttt tcaggatccg gcatgttggc cggatcagga
    19921 acgacaaaac aatggcctgg aggctacctt gttaaattca tttaaacttt cgctacagta
    19981 cattctgccg aaactatggc ttactcgcct ggcgggttgg ggcgcaagca agcgggcagg
    20041 atggctgaca aaactggtta tcgatctgtt cgttaaatac tacaaggtcg acatgaaaga
    20101 ggcgcaaaag ccggacaccg ccagctaccg cacctttaac gaattctttg tccgtccgct
    20161 gcgtgacgaa gtacgcccaa tcgataccga tccgaatgta ctggtcatgc ctgccgatgg
    20221 cgttatcagc cagttgggta aaatcgaaga agataaaatc ctgcaagcca aaggccacaa
    20281 ctacagcctc gaagccctgc tggcaggcaa ctatctgatg gcggacctgt tccgcaacgg
    20341 tacgtttgtg accacttacc tctccccgcg tgactaccat cgcgtacaca tgccgtgcaa
    20401 cggtattctg cgtgagatga tctacgtgcc gggcgatctc ttctccgtta accatctcac
    20461 cgctcagaac gtgccgaatc tgtttgcccg taacgaacgc gtgatttgcc ttttcgatac
    20521 cgaatttggc ccaatggcgc agattctggt cggagcgacg attgttggca gcattgagac
    20581 ggtctgggcg ggtaccatta cgccgccgcg cgaaggtatc atcaagcgct ggacctggcc
    20641 tgccggggaa aacgacggtt ctgtggcact gctgaaaggc caggaaatgg gtcgctttaa
    20701 actcggttcc accgttatca acctgtttgc agcgggtaaa gtgaatctgg ttgagcaact
    20761 ggaaagcctg tctgtcacga aaattggcca gccgctggca gtatctaccg aaacctttgt
    20821 tacgccagac gctgaacccg ccccacttcc tgctgaagag atcgaagcag aacacgacgc
    20881 cagcccattg gttgacgata aaaaagacca ggtctaatcc atcaaaggaa acgctgacgt
    20941 gcgcctgatt atcacttttc tgatggcctg gtgcctcagt tggggggcgt acgccgcgac
    21001 ggcccccgat agcaaacaaa tcactcagga actggagcag gcaaaagcgg cgaaacccgc
    21061 acagccggaa gtcgtagagg cgctccagtc tgccttaaat gcgcttgagg aacgaaaagg
    21121 ttcccttgag cgcatcaaac aatatcagca agttatcgat aattatccga aactctccgc
    21181 tactctgcgc gcacaattaa acaacatgcg tgacgagccg cgcagcgtgt cgccgggaat
    21241 gtctaccgac gcgctgaatc aggaaattct ccaggtcagc agccagttgc tggataaaag
    21301 ccgtcaggcc cagcaagagc aggagcgcgc ccgcgagatt gccgattcgc tgaatcaact
    21361 gccgcaacag caaaccgacg cccgccgtca gttaaatgag atcgagcgcc gcctgggaac
    21421 gcttaccggc aatactccgc tcaatcaggc acaaaatttc gcgttgcagt ctgactctgc
    21481 acgtcttaag gcgctcgttg atgaactgga gctggcgcag ctgtctgcca ataaccgcca
    21541 ggaattagcg cgcttacgct cagagctggc ggaaaaagag agccagcaac tggatgcgta
    21601 tttgcaggcc ttgcgtaatc aattaaacag ccaacgtcag cttgaggcgg agcgggcgct
    21661 ggaaagtacc gaattgctgg cagaaaacag cgccgatttg ccgaaagata tcgtcgcgca
    21721 attcaaaatt aaccgcgaac tatcggcggc tttgaatcaa caggcgcagc ggatggatct
    21781 cgttgcctcg caacagcgtc aggctgccag ccagacgtta caggtccggc aggcgttgaa
    21841 tacgctgcgt gaacagtcgc aatggctggg atcgtccaat ctgctcggcg aagcgctgcg
    21901 ggcgcaggtg gcacggctgc cagaaatgcc aaaaccacaa cagcttgata ccgaaatggc
    21961 gcagttgcgt gtgcaacggt tacgttatga ggatctgctt aataaacagc cgctgctacg
    22021 gcaaatccat caggccgacg ctcagccgct gaccgccgag caaaaccgta ttctggaagc
    22081 acaactgcgc actcagcgtg agttgctgaa ctcattgttg cagggtggcg acacgctact
    22141 attggaactg accaagctga aagtctccaa cgggcaactg gaggatgcgc tgaaagaggt
    22201 gaacgaagca acgcaccgct atctgttctg gacctctgac gtgcgcccga tgaccatcgc
    22261 ctggccgctg gaaatcgccc aggatctgcg tcgtctcatt tcgctggaca ccttcagtca
    22321 gttgggcaaa gccagtgtga tgatgctgac cagcaaagag acaattttgc cgctgtttgg
    22381 cgcgttgatt ctggtcggtt gcagtattta ctcgcgccgc tatttcaccc gttttcttga
    22441 acgttcggcg gcgaaagttg gcaaagtgac tcaggatcac ttctggctga cgttgcgcac
    22501 tcttttctgg tcgattctcg tcgcgtcacc gttaccggtg ctgtggatga cgctgggtta
    22561 cggcttgcgc gaggcgtggc cttatccgct ggcggtcgcg attggcgatg gtgtaacggc
    22621 caccgtgccg ctgctgtgga tagtgatgat ttgcgccacc tttgcccgcc cgaacggctt
    22681 gtttatcgct cattttggct ggccgcgcga acgtgtttcc cgtgggatgc gctactacct
    22741 gatgagcatc gggcttattg tgccgctgat tatggcgctg atgatgttcg ataacctcga
    22801 cgaccgtgaa ttctccggtt cgctgggacg gctttgcttt atcctcattt gcggtgcgct
    22861 ggcggtggtc accctcagcc tgaaaaaggc cgggatccca ctgtatctca acaaagaggg
    22921 cagcggcgac aacattacca accatatgct gtggaacatg atgattggcg cgccgttggt
    22981 tgccattctg gcgtcggctg tgggttatct ggcaacggca caggcgctgt tagcgaggct
    23041 tgaaacctcg gttgccatct ggttcctgct actggtggtt tatcacgtta tccgccgctg
    23101 gatgctgatc cagcgccgca ggctggcgtt tgaccgggcg aagcatcgcc gggcagagat
    23161 gttagcgcaa cgtgcgcgtg gcgaagagga agcgcatcat cacagtagcc cggaaggagc
    23221 aattgaagtc gatgaaagcg aagtcgatct cgatgcaatc agtgcgcaat ccttgcggct
    23281 ggtgcgctca attttgatgt tgatcgccct gctttctgtc attgtgctgt ggtcagaaat
    23341 ccattccgct ttcggcttcc tcgaaaatat ttcgctgtgg gatgtcacct ccacggtaca
    23401 gggcgtagaa agtctggagc caattaccct cggtgcggtg ctgattgcca ttctggtgtt
    23461 tatcatcacc acgcagctgg tgcgcaacct gcccgcgctg ctggaactgg cgattttgca
    23521 gcacctggat ttaacgccgg gtacgggcta cgccatcacc accatcacca aatatctgct
    23581 gatgctgatt ggcgggctgg tcggcttctc gatgattggt attgagtggt cgaaattgca
    23641 gtggctggtc gccgcgctcg gtgttggtct cggttttggt ttgcaggaaa ttttcgccaa
    23701 ctttatctct ggtctgatta tcctgttcga aaaaccgatt cgcattggcg atacggtgac
    23761 aattcgcgat ctcaccggta gcgtgacgaa aattaacacc cgcgccacca ccatcagcga
    23821 ctgggaccgt aaagagataa tcgtgccgaa caaggcgttt attaccgagc agtttatcaa
    23881 ctggtcgctc tctgactcgg tcacgcgcgt agtgttgacg attccggccc ctgctgatgc
    23941 caacagtgaa gaagtgacgg aaatcctgct caccgcagcg cgtcgctgct cgctggtgat
    24001 cgacaacccg gcaccggaag tcttcctggt ggatctgcaa caagggattc agattttcga
    24061 gctgcgtatt tatgccgctg agatgggtca ccgtatgccg ctacgccatg agatccacca
    24121 gctgattctg gctggcttcc atgcccacgg tatcgatatg ccattcccgc ccttccagat
    24181 gcgtctggaa agcctcaacg gtaaacaaac ggggagaacg ctgacgtctg cgggcaaagg
    24241 tcgtcaggcg ggaagtttgt aatgcatgaa gctgccaaat gatgtcaacg tggctcattt
    24301 agcagcttaa catcatcttc agttatcacc ggccctggag agtatcatcc atacgcatta
    24361 cttcctgaca actcatcgcc ggagagacga tgagttgcag gatttcatat gatcagtcga
    24421 gagctaactc aggtttattc gccatgcgac tgcaatagtt ctggtaaatc gccatcgcta
    24481 acagcgagaa gaagacaggg ccgccaatca tccacaatgt gctgtcccag tctccggctt
    24541 caaccacagg ctgaataatg gtgaagacgt tcgcaaatgt caccaccagt acgacaacca
    24601 ccgtcgcaat cattgccgac atacgcgttt tgaaaatcac aaatggtctg tcgagatcct
    24661 gacgtgcttt aaagaatggg aaagccagcg cgaggaacag gtaaggaagc gtcatagaca
    24721 cgttcgccat cagcgtcagc ttgttaaaga acgcggatgc ggtaccgcca ccaaacgaaa
    24781 ccagcaggat gaagacagta accaacccgc actgcatcca catggcgata gacggcatcc
    24841 ccatcgcatt caggcgcgtc atcggttccg gccacaacgc tttcggcgtc ccctggatga
    24901 tggctttcag cggtgaatag caaagcgtaa agaacgcacc ggtataagcg aggaacatcg
    24961 aaagtccggt aatacgcgca aaccatacgc ccagcgacaa tgacgcttca ggtgacaaat
    25021 gcagtgcgtt acccagcgtc accccgaggc tcttcatcag cacataggta atattgccaa
    25081 ggttaacgga accattactt aatacctgct gccagtttgt gctgacgccc cataaaaata
    25141 ttgccagcga ataaccgatt gaaataacaa tagcggcaaa aacaatacct ttggcaaagt
    25201 tcttttctgg attttccgtt ttatcgacca gaccaccaac cgcttcaatt ccgccatagg
    25261 caaaaatagc aaataccaca aacgatagca ttgccagacc ggactgataa cccgggttcg
    25321 gtgatgcaag gaaattaata tcctgcgcga aatgcccacc atttaataac aaaatagtaa
    25381 tgcttactaa cagcaatact aaattcagac acattactgc aataccgccc accgcagtaa
    25441 tgcgggcaat tttattaatc ccctttgaag caacgacggt gaccagaatc atccatgcta
    25501 ctgccagcag accaaccacc tgcgtaggct ccagtccggc aatacgccag tgctgggtca
    25561 tgtcgctacc atagaggaat gttgagaacg gtacccaaac tttcgcggag gtactcacca
    25621 tccagatgat ataagaggaa aaccacataa acgtaccaat gaaggcaaaa cgtggtccga
    25681 cactattatt catccaggaa tagataccac cttcttcttt gcgataagca gctcccattt
    25741 cagccatcat taaggcgaat ggaataaaga ataacaatgc agaaaatata taaaagggaa
    25801 tcgcactata acccattaag taataagccg atgggctatt ggcaaatcca aatacggaag
    25861 taaagatcat caatatgagt cctatcagac tcatcttttt tatcgtgtga ggcatgaaac
    25921 catccttcat ttcagcgcgt gtataacacg tgcccactga actgcactgc gcaacattga
    25981 gcaggcagcg taaaaaccaa tacagaattg atacctggat gatatcaaat atccagtctt
    26041 aaattgtggc ctttaacgga aaaatgaaaa catatgctac agaatggcgc ttatcacgcc
    26101 attcttcgct gttaattcag taatttttca gaattatgcc cggtcaacgc taaaggcgat
    26161 gacttcagcc agtgtctccg cgcccagcgc caacatcacc agacgatcaa cacctaatgc
    26221 cacgccggaa cagtcaggca taccgacttt caaggcttca atcagattct ggtcaatggg
    26281 gtgctgcggc aaaccgcgcg ccgcgcgctt acggttatct tgttcaaagc gttgttgctg
    26341 ctcacgggca tccgttaatt catggaaacc attcgccagc tcaatacctt tataataaac
    26401 ctcaaagcgt tcagcgaccc gatgatcttc ggtactgatt tgcgccagtg atgcctggct
    26461 ggctggaaag tggtacacaa aggtcggttt ttctttacca atatttggct ctacgccaaa
    26521 ggtaaacagc aattgtagca acgtgtcgcg gtcttcttcg gtatcagcaa cgttactcaa
    26581 atccagtttc gctgcgactt cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc
    26641 cagataacgc aagaaagctt gttgataaga aaggctttct gctgccgggc agtccagcac
    26701 ctgttgtaag agatcgtcca cctcgttcat caaccggtac atatcatagt gcggtcgata
    26761 ccactcaagc atagtgaact cagggttgtg ataacgcccc atctcttcat tacggaagct
    26821 gcggcacagc tggaataccg gcccacaacc ggcaaccagc aggcgtttca tatggtattc
    26881 cgggctggtc attaaccaga gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt
    26941 ctcaaacggg accaaatgaa tatcggttac cgtcgcctgg ctcatacaag gcgtctccac
    27001 ctccagcact ccacgatcgg caaagaaacg acggatctcc gccataatcg ccgcgcgttt
    27061 taataagtta ggaatggatg cgctcggctg ccaggatgcc gtttcgctca tagttaaatc
    27121 tccagttttt gacaagggca cgaagtctac tcgcaacgcg acggcgagac aaattttacg
    27181 caggaatcaa acagcggtgg gcagtgacta aaaaaagcac gatctgatgg tttagtaatt
    27241 aaattaatca tcttcagtga taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc
    27301 aaatttcaga cttatccatc agactatact gttgtaccta taaaggagca gtggaatagc
    27361 gttcgcagac cgtaactttc aggcacttac cctgaagtac ggggctgtgg gataaaaaca
    27421 atctggagga atgtcgtgca aacctttcaa gccgatcttg ccattgtagg cgccggtggc
    27481 gcgggattac gtgctgcaat tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta
    27541 atctcaaaag tatacccgat gcgtagccat accgttgctg cagaaggggg ctccgccgct
    27601 gtcgcgcagg atcatgacag cttcgaatat cactttcacg atacagtagc gggtggcgac
    27661 tggttgtgtg agcaggatgt cgtggattat ttcgtccacc actgcccaac cgaaatgacc
    27721 caactggaac tgtggggatg cccatggagc cgtcgcccgg atggtagcgt caacgtacgt
    27781 cgcttcggcg gcatgaaaat cgagcgcacc tggttcgccg ccgataagac cggcttccat
    27841 atgctgcaca cgctgttcca gacctctctg caattcccgc agatccagcg ttttgacgaa
    27901 catttcgtgc tggatattct ggttgatgat ggtcatgttc gcggcctggt agcaatgaac
    27961 atgatggaag gcacgctggt gcagatccgt gctaacgcgg tcgttatggc tactggcggt
    28021 gcgggtcgcg tttatcgtta caacaccaac ggcggcatcg ttacaggtga cggtatgggt
    28081 atggcgctaa gccacggcgt tccgctgcgt gacatggaat tcgttcagta tcacccaacc
    28141 ggtctgccag gttccggtat cctgatgacc gaaggttgcc gtggcgaagg cggtattctg
    28201 gtcaacaaaa atggctaccg ttatctgcaa gattacggca tgggcccgga aactccgctg
    28261 ggcgagccga aaaacaaata tatggaactg ggtccacgcg acaaagtttc tcaggccttc
    28321 tggcacgaat ggcgtaaagg caacaccatc tccacgccgc gtggcgatgt ggtttatctc
    28381 gacctgcgtc acctcggcga gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg
    28441 gcgaaagcgt acgttggcgt cgatccggtt aaagaaccga ttccggtacg tccgaccgca
    28501 cactacacca tgggcggtat cgaaaccgat cagaactgtg aaacccgcat taaaggtctg
    28561 ttcgccgtgg gtgaatgttc ctctgttggt ctgcacggtg caaaccgtct gggttctaac
    28621 tccctggcgg aactggtggt cttcggccgt ctggccggtg aacaagcgac agagcgtgca
    28681 gcaactgccg gtaatggcaa cgaagcggca attgaagcgc aggcagctgg cgttgaacaa
    28741 cgtctgaaag atctggttaa ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa
    28801 atgggtctgg ctatggaaga aggttgcggt atctaccgta cgccggaact gatgcagaaa
    28861 accatcgaca agctggcaga gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc
    28921 tccagcgtgt tcaacaccga cctgctctac actattgaac tgggccacgg tctgaacgtt
    28981 gctgaatgta tggcgcactc cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt
    29041 ctggacgaag gttgcaccga gcgtgacgac gtcaacttcc tcaaacacac cctcgccttc
    29101 cgcgatgctg atggcacgac tcgcctggag tacagcgacg tgaagattac tacgctgccg
    29161 ccagctaaac gcgtttacgg tggcgaagcg gatgcagccg ataaggcgga agcagccaat
    29221 aagaaggaga aggcgaatgg ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa
    29281 cccggaagtc gataccgcac cgcatagcgc attctatgaa gtgccttatg acgcaactac
    29341 ctcattactg gatgcgctgg gctacatcaa agacaacctg gcaccggacc tgagttaccg
    29401 ctggtcctgc cgtatggcga tttgtggttc ctgcggcatg atggttaaca acgtgccaaa
    29461 actggcatgt aaaaccttcc tgcgtgatta caccgacggt atgaaggttg aagcgttagc
    29521 taacttcccg attgaacgcg atctggtggt cgatatgacc cacttcatcg aaagtctgga
    29581 agcgatcaaa ccgtacatca tcggcaactc ccgcaccgcg gatcagggta ctaacatcca
    29641 gaccccggcg cagatggcaa agtatcacca gttctccggt tgcatcaact gtggtctgtg
    29701 ctacgccgcg tgcccgcagt ttggcctgaa cccagagttc atcggtccgg ctgccattac
    29761 gctggcgcat cgttataacg aagatagccg cgaccagggt aagaaggagc gtatggcgca
    29821 gttgaacagc cagaacggcg tatggagctg tactttcgtg ggctattgct ccgaagtctg
    29881 cccgaaacac gtcgatccgg ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga
    29941 ctttcttatc gcgaccctga aaccacgcta aggagtgcaa catgacgact aaacgtaaac
    30001 cgtatgtacg gccaatgacg tccacctggt ggaaaaaatt gccgttttat cgcttttaca
    30061 tgctgcgcga aggcacggcg gttccggctg tgtggttcag cattgaactg attttcgggc
    30121 tgtttgccct gaaaaatggc ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc
    30181 cggttatcgt gatcattaac ctgatcactc tggcggcagc tctgctgcac accaaaacct
    30241 ggtttgaact ggcaccgaaa gcggccaata tcattgtaaa agacgaaaaa atgggaccag
    30301 agccaattat caaaagtctc tgggcggtaa ctgtggttgc caccatcgta atcctgtttg
    30361 ttgccctgta ctggtaagga gcctgagatg attaatccaa atccaaagcg ttctgacgaa
    30421 ccggtattct ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg
    30481 atgatcctgc tggtgggtat tctgctgcca ctggggctgt ttccgggtga tgcgctgagc
    30541 tacgagcgcg ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg
    30601 atcgttctgc cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa
    30661 attcacgtgc ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc
    30721 acgctgattg gtatcgttac aatctaacgt atcgccaatg taaatccggc ccgcctatgg
    30781 cgggccgttt tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa
    30841 ttaccgcctc ttgctccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca
    30901 caattacccc gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc
    30961 agggtaaacc ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca
    31021 cacagcaaac gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg
    31081 gcgacgctat tgctcgaggg gaaatcaagt taagcgatcc cacaacaaaa tactggcctg
    31141 aacttaccgc taaacagtgg aatgggatca cactattaca tctcgcaacc tacactgctg
    31201 gcggcctgcc attgcaggtg ccggatgagg tgaaatcctc aagcgacttg ctgcgcttct
    31261 atcaaaactg gcagcctgca tgggcgccag gaacacaacg tctgtatgcc aactccagta
    31321 tcggtttgtt cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc
    31381 aaacacgtgt cttccagcca ctcaaactca accatacgtg gattaatgta ccgcccgcag
    31441 aagaaaagaa ttacgcctgg ggatatcgcg aaggcaaggc agttcatgtt tcgccagggg
    31501 cgttagatgc tgaaacttat ggtgtgaagt cgaccattga agatatggcc tgctgggtac
    31561 gaagcaatat gaatccccgt gatatcaacg acaaaacact tcagcaaggg atacaactgg
    31621 cacaatctcg ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg
    31681 actggccggt aaatcctgac agcatcatta acggcagtgg caataaaatt gcactggcag
    31741 cacaccccgt aaaagcgatt acgcccccaa ctcctgcagt acgcgcatca tgggtacata
    31801 aaacaggggc aaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg
    31861 gtatagtgat gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctggc
    31921 agattctcaa cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg
    31981 cttttccttg ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg
    32041 ctccctctcg ttgccgcagc gacagctgca tttctggtcg ttgcctgcag ttctcctacg
    32101 ccgccgcgtg gcgtaacagt agtgaataat ttcgacgcca aacgttatct cggtacctgg
    32161 tatgagattg cccgttttga tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca
    32221 tacagcctgc gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga
    32281 ggaatgtggc agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg
    32341 ctgaaagtgt cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg
    32401 gaataccgcc atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc
    32461 acgccaacca tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg
    32521 tttgatgtca gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc
    32581 agaccaataa tccccaatac gattaacgcc agactcgcca ggcgcatcgg gttagcggac
    32641 tcaccgagca gcacaatgcc ggtgatagcc gcgccgactg cgccaatacc cgtccacacg
    32701 gcataagccg tccctactgg taacgatttc atcgcccagg caagtagcgc catactgaca
    32761 atcatcgccg tcacggtaat aacactcggc gtcaaacgac taaagccgtg ggtatatttc
    32821 aggccaacgg cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata
    32881 tcaggctccg gagaattggg gccgtccccg gtgaaaagac gcgtttgcag gtcgtcctgc
    32941 aaagctgatg gttgaacgga tattttgcca ggtggcaggg gaatttcaac tattacctta
    33001 gtgactatcg cagaaaaaac aaacggcacg acatacctgt cgtacagtta ttgcttattg
    33061 ctattgctgc gctttcgttg ctgcgccaga aatcgcgtta ccgccatcag aaatgtcttc
    33121 accaacgcca cgcgtggtgt tgcaggcagt taatactgtt gaaagcacca gaacagaaaa
    33181 gatcgctgca attgtcttct tcaccataac gtcttccttt taggtaatgt tatttatgtt
    33241 tgcctatagc aaattaataa tagacaacat cgccaaaaat gacggaaatc tgatgatttt
    33301 tcggaagaga agaaaaatta gctggcagcg cgagagatgg agttgccgag atgtttgatg
    33361 tcttcgccga aaccgcgagc ggtgttacag cccgtgagca gcgtgctggc aagcaaaaca
    33421 agaacgataa ggcgtttcat catctctgac ctgaataagt gatggtgcag cctgcaggcc
    33481 gcaccacaac cgcattactt cacgcgagag acgtattcac cagagcgggt atccactttg
    33541 atgacttcgc cgatttgtac gaacagcgga accttaacca cagcgccagt agacagggta
    33601 gccggtttgc cgccagtacc tgcggtatca cctttcaggc ccggatcggt atcaacgatt
    33661 tccagttcaa cgaagttcgg cggagtaacg gagatcggct gaccattcca cagagttacg
    33721 atacactctg cctgatccag cagccattta gcgttatcgc caattgcttt tgcatcagca
    33781 gacagctgct cgaaagtttc gttgttcatg aagtgccaga actcaccgtc gttgtacagg
    33841 taagtcaggt tcatatcgac aacatcagcg ccttcagcgg aatcagtaga tttgaaggtt
    33901 ttttctacgc gagtaccggt cagcagacga cgcagtttaa cgcgagcaaa tgcctggcct
    33961 ttacccggtt ttacgaattc actcgcttca accgcgtaag gttcgccgtc taacatgatt
    34021 ttaagaccag cacgaaaatc gttgctatag tacgttgcca taaggccctc tgaaatttgt
    34081 taattggtag ctaagccaca aaatggcgca tattgtaacc ctaaataccc catccagaga
    34141 agattggtta acgcaacttg ccgatgttgt gaccgatcct gatgaacttc tgcgtctttt
    34201 gaatatagac gcggacgaaa aactgttggc cggacgcagc gccaaaaagc tgtttgccct
    34261 gcgtgtgccc cgctcattta tcgatcgcat ggagaaaggc aatccggacg atcctatttt
    34321 gcgtcaggta cttacctcgc aagatgagtt tgtcgtcgcg tccggattct ccaccgaccc
    34381 gctggaagaa cagcacagcg tagtgcctgg tttgttgcat aaataccaca accgggcgct
    34441 tttgctggtc aaaggcggct gcgcggtaaa ttgccgctat tgcttccgtc gccacttccc
    34501 ctatgccgaa aatcagggca acaagcgtaa ctggcagact gctcttgagt atgttgctgc
    34561 gcatccggaa ctggacgaga tgattttctc cggcggcgat ccgctgatgg cgaaagatca
    34621 cgagctggac tggttgctca cacaactgga agccatcccg catatcaaac gtctgcggat
    34681 tcacagccgt ctgccgattg tgatcccggc acgtatcacc gaggcgctgg ttgaacgctt
    34741 tgcccgttct acgctgcaaa tcttgctggt gaatcacatc aatcatgcca atgaggtaga
    34801 tgaaacgttc cgtcaggcga tggctaaatt gcgccgtgtc ggtgtcaccc tgcttaacca
    34861 gagcgttctg ttacgtggtg tgaacgataa cgcacaaacg ctggcaaacc tgagtaatgc
    34921 gttgttcgat gccggcgtaa tgccgtatta cctgcatgtg ctcgataaag tacagggcgc
    34981 ggcgcatttt atggtgagtg atgacgaagc acggcagatt atgcgtgagt tgctgacact
    35041 ggtgtcgggt tatctggtgc cgaaactggc gcgagaaatc ggcggcgaac ccagcaaaac
    35101 gccgctggat ctccagctac gccagcagta actgattaaa cagggaagtt aaacattaaa
    35161 attgcctgat gcgctacgct tatcaggcct acatcgtgca ttgaaattga atggatttgc
    35221 aatgaactgt aggccggata aggcgtttac gccgcatccg gcaagagcaa caaacgcaag
    35281 tacagcaacc tgtcattaaa ctgagaaatt taatctttgc ccgttgcata acctttaatt
    35341 attcatatgc attgctgttg ttacttttta tatagctaac accaa
//
LOCUS       DAZKHC020000042        35193 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZKHC020000042 DAZKHC020000000
VERSION     DAZKHC020000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.042"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            861..1685
                     /gene="dinD"
                     /locus_tag="ACHOSM_RS11905"
                     /old_locus_tag="pgaptmp_002381"
     CDS             861..1685
                     /gene="dinD"
                     /locus_tag="ACHOSM_RS11905"
                     /old_locus_tag="pgaptmp_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HGU4558889.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKNQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            1977..2594
                     /gene="yicG"
                     /locus_tag="ACHOSM_RS11910"
                     /old_locus_tag="pgaptmp_002382"
     CDS             1977..2594
                     /gene="yicG"
                     /locus_tag="ACHOSM_RS11910"
                     /old_locus_tag="pgaptmp_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HGU4558890.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(2591..4273)
                     /gene="ligB"
                     /locus_tag="ACHOSM_RS11915"
                     /old_locus_tag="pgaptmp_002383"
     CDS             complement(2591..4273)
                     /gene="ligB"
                     /locus_tag="ACHOSM_RS11915"
                     /old_locus_tag="pgaptmp_002383"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HGU4558891.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKIGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            4531..5154
                     /gene="gmk"
                     /locus_tag="ACHOSM_RS11920"
                     /old_locus_tag="pgaptmp_002384"
     CDS             4531..5154
                     /gene="gmk"
                     /locus_tag="ACHOSM_RS11920"
                     /old_locus_tag="pgaptmp_002384"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGU4558892.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            5209..5484
                     /gene="rpoZ"
                     /locus_tag="ACHOSM_RS11925"
                     /old_locus_tag="pgaptmp_002385"
     CDS             5209..5484
                     /gene="rpoZ"
                     /locus_tag="ACHOSM_RS11925"
                     /old_locus_tag="pgaptmp_002385"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGU4558893.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            5503..7611
                     /gene="spoT"
                     /locus_tag="ACHOSM_RS11930"
                     /old_locus_tag="pgaptmp_002386"
     CDS             5503..7611
                     /gene="spoT"
                     /locus_tag="ACHOSM_RS11930"
                     /old_locus_tag="pgaptmp_002386"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HGU4558894.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            7618..8307
                     /gene="trmH"
                     /locus_tag="ACHOSM_RS11935"
                     /old_locus_tag="pgaptmp_002387"
     CDS             7618..8307
                     /gene="trmH"
                     /locus_tag="ACHOSM_RS11935"
                     /old_locus_tag="pgaptmp_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HGU4558895.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITEEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            8313..10394
                     /gene="recG"
                     /locus_tag="ACHOSM_RS11940"
                     /old_locus_tag="pgaptmp_002388"
     CDS             8313..10394
                     /gene="recG"
                     /locus_tag="ACHOSM_RS11940"
                     /old_locus_tag="pgaptmp_002388"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGU4558896.1"
                     /translation="MKGRLLDAVPLNSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQVMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(10379..11245)
                     /locus_tag="ACHOSM_RS11945"
                     /old_locus_tag="pgaptmp_002389"
     CDS             complement(10379..11245)
                     /locus_tag="ACHOSM_RS11945"
                     /old_locus_tag="pgaptmp_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4558897.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASLRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(11248..12453)
                     /gene="gltS"
                     /locus_tag="ACHOSM_RS11950"
                     /old_locus_tag="pgaptmp_002390"
     CDS             complement(11248..12453)
                     /gene="gltS"
                     /locus_tag="ACHOSM_RS11950"
                     /old_locus_tag="pgaptmp_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HGU4558898.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            12733..14124
                     /gene="xanP"
                     /locus_tag="ACHOSM_RS11955"
                     /old_locus_tag="pgaptmp_002391"
     CDS             12733..14124
                     /gene="xanP"
                     /locus_tag="ACHOSM_RS11955"
                     /old_locus_tag="pgaptmp_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HGU4558899.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            14245..15954
                     /gene="yicH"
                     /locus_tag="ACHOSM_RS11960"
                     /old_locus_tag="pgaptmp_002392"
     CDS             14245..15954
                     /gene="yicH"
                     /locus_tag="ACHOSM_RS11960"
                     /old_locus_tag="pgaptmp_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU4558900.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISTWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGDVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDIRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(16007..18325)
                     /gene="yicI"
                     /locus_tag="ACHOSM_RS11965"
                     /old_locus_tag="pgaptmp_002393"
     CDS             complement(16007..18325)
                     /gene="yicI"
                     /locus_tag="ACHOSM_RS11965"
                     /old_locus_tag="pgaptmp_002393"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HGU4558901.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAVRTGNTITVTGAGETKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(18335..19717)
                     /gene="yicJ"
                     /locus_tag="ACHOSM_RS11970"
                     /old_locus_tag="pgaptmp_002394"
     CDS             complement(18335..19717)
                     /gene="yicJ"
                     /locus_tag="ACHOSM_RS11970"
                     /old_locus_tag="pgaptmp_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HGU4558902.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            20010..20104
                     /locus_tag="ACHOSM_RS11975"
                     /old_locus_tag="pgaptmp_002395"
     tRNA            20010..20104
                     /locus_tag="ACHOSM_RS11975"
                     /old_locus_tag="pgaptmp_002395"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20044..20046,aa:Sec,seq:tca)
     gene            20404..21585
                     /locus_tag="ACHOSM_RS11980"
                     /old_locus_tag="pgaptmp_002396"
     CDS             20404..21585
                     /locus_tag="ACHOSM_RS11980"
                     /old_locus_tag="pgaptmp_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4558903.1"
                     /translation="MALTDIKVKTAKPKEKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGMAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLACALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIALFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEIQLAHVDKNAIRGTYNHAQYLDGRREMMQWYADYIDSLSEQ
                     I"
     gene            21690..22142
                     /locus_tag="ACHOSM_RS11985"
                     /old_locus_tag="pgaptmp_002397"
                     /pseudo
     CDS             21690..22142
                     /locus_tag="ACHOSM_RS11985"
                     /old_locus_tag="pgaptmp_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            23002..23106
                     /locus_tag="ACHOSM_RS11990"
                     /old_locus_tag="pgaptmp_002398"
                     /pseudo
     CDS             23002..23106
                     /locus_tag="ACHOSM_RS11990"
                     /old_locus_tag="pgaptmp_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014657360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
     gene            23543..24073
                     /locus_tag="ACHOSM_RS11995"
                     /old_locus_tag="pgaptmp_002399"
     CDS             23543..24073
                     /locus_tag="ACHOSM_RS11995"
                     /old_locus_tag="pgaptmp_002399"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4558904.1"
                     /translation="MVALLVGHGAFTLYPALSQCDNAAERLTRWLTYQLPRVKQAHPG
                     CPIHQWLNSIGAQVIVEKWQSPEQWPNLYWLPLPDVDGNAQGGLALLPAHLLPADFLS
                     VYVDGTIQTRTLQANTPANTCIFWCITPLSKTGKRATLHPKQQNRAFCLPSSPEQMGK
                     SCTGLLLSGLRKDNGK"
     gene            complement(24262..27000)
                     /locus_tag="ACHOSM_RS12000"
                     /old_locus_tag="pgaptmp_002400"
     CDS             complement(24262..27000)
                     /locus_tag="ACHOSM_RS12000"
                     /old_locus_tag="pgaptmp_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017244827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGU4558905.1"
                     /translation="MTMDLSSLKPDQLRAINNIIGRLSLRQPQQDSLRALAVAISASV
                     HKLLNPKRDVPELLETLKAQFPKLSDFERDFPSLCFALATGVGKTRLMGAFITYLYQV
                     HGIRNYFVLAPNLTIYEKLISDFSPASPKYVFKGITDFVLAPPEVITGDNYESRGAQV
                     MGDLFGSVRINVFNIAKINTEVRGGKAPRIKRLAETLGESYFEYLSNLPDLVLLMDES
                     HRYRADAGMRALNELNPLFALELTATPFTESSRGPVHFKNVVIDYPLACAMDDGFVKE
                     PAVVTQRNFDASQYDKDELETIKLMDGIRLHEQTKVDLITYAHENDAKLVKPFMLVIA
                     RDTTHAAELLTKLETVFDGRYRGKVIQVDSSKKEEETIQALLNVEHVDEPTEIVIHVN
                     MLKEGWDVTNLYTIVPLRAANARTLIEQSIGRGLRLPYGKRTGVEAVDTLSIVAHDKF
                     QEIVDEANDPNNPLRLKTRVLDRPEDDVTKQSVQVKSNLEQQLTGSGVTATGADGNRE
                     VQEPKATYTTPNQQTVARAALDVIKQYQTRPTDAPTSKALLTTEVKAQITEQVKAQLT
                     QEQRELFTEESVDIAAIVEQVTQLTVDNTIDIPRIIVSPSGEVRTGYHPFDLDIAAIK
                     GLKPIDRTLIQQSLQDNHQTIRGEGDSGNYEPVKQNYILKKLFDYDDIPYDEQAELLF
                     DLATQAVNTLAAENDDDVVENILQNQSDAIAKLIHAQLNNHFFEEATEYVVDVRSGFS
                     TLRDCAYTIAQDQPVQSYRDTVRDVSRIKQMLFGGFSRCLYPLQKFDSDTERRFAVIL
                     ERDCEKWFKPAKGQFKMYYKRGSEHPEYVPDFVAETADYVLMIETKSTQHQNQDGSWN
                     EEVTEKARSGVKWCSHASEYLAQHGGKPWKYLLIPHDAVKEANSLAYFTQQFAKAE"
     gene            complement(27022..28725)
                     /locus_tag="ACHOSM_RS12005"
                     /old_locus_tag="pgaptmp_002401"
     CDS             complement(27022..28725)
                     /locus_tag="ACHOSM_RS12005"
                     /old_locus_tag="pgaptmp_002401"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGU4558906.1"
                     /translation="MSKTKLELTWIGKNERKKLEPRILLEDPSKSYHASQKVTENDIF
                     DNKLIFGDNLLALKALEQEYAGKVKCVYIDPPFNTGSAFEHYDDGLEHSIWLGLMRDR
                     LEIIRNLLSDDGVIFVHLDDCEMAYLKVLMDEIFGRANQLNTISMTTNDPSGFKATGA
                     TVFSTVNFLLVYAKDRTQKPLNKIYIPKGYDTGYNKYLLNPDDHYKSWTWCGIADAFA
                     KANGYKDTKEAKKKLPDTFDDELAQFAIDNAERVFQSVAIGGGAKIKRKETIEKSKKD
                     RDKVYVHPNEDVEGFYIANGRQMVFYSNRLLEIDGEMQPAELITDVWTDISWNGIANE
                     GGVSFKNGKKPEALLKRIFEMCTREGDLVFDSFGGSGTTAAVAHKMKRRWISVELGDH
                     CHTHIIPRLQKVIDGEDQGGISKSVNWQGGGGFRYYKLAPTLIVKDKFGLEVINKEYN
                     PEMLAEAVCKLEGFTYAPSEVNWWDHGYSTETDHIYVTTQSLSVDKLEALSEEVGSDR
                     TLLVMCGAFRCEPGRFTNLTLKKLPKAILDKCQFGQDDYSLNVANLTDDASADDDAAQ
                     D"
     gene            complement(28783..30621)
                     /locus_tag="ACHOSM_RS12010"
                     /old_locus_tag="pgaptmp_002402"
     CDS             complement(28783..30621)
                     /locus_tag="ACHOSM_RS12010"
                     /old_locus_tag="pgaptmp_002402"
                     /inference="COORDINATES: protein motif:HMM:NF022062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HGU4558907.1"
                     /translation="MAALQPQFLTPLKELLQQRFVPHLPPLLGQGGREDKPKKQISRA
                     FSAFVLQQKFDLDVVTAAKAVVDDYEDHGIDAIYYHEADQTLYLVQSKMKEDAQFQLG
                     EAQAFIEGVKLLLNKQFHRFNQNVQNLQANIEAALDECESIQLLVAYTGNGITIQAQN
                     YLQPAIQELIDEGEEQIAPEYQEYTATDVEQALRSEHAVDTVNERVAVYKFRVQEQPR
                     KVVFGIAKLTDLIALHTTHDRKLYEKNIRYFIGAGRRGVNRAIKDTLLHEPENFIYLN
                     NGITLVGNAVKPKGNIRGHTGSKHVAVDGLSVVNGAQTIASAAQFMRENPNADISQAQ
                     VMVTIINTGNDAFHKQVTKARNLQNPVDLANFAALDDTQERLRQEMKMFGVEYLYRPQ
                     QSAAAGIRSITIDTLAKALACMQGDVRVPYQLKVEPSKFTNQESAEYQAVFSTDLQGA
                     VAINAVNCYLAIQVLCTNAERSSPSPEKLVYRHFTYCITTLLMAQFEGAITAPEITEQ
                     AAFKRLISHAFDELRQQFFDSFTALVLGSAPHAYFKRLGDTARLMQTVWISHLNLAES
                     PAVVAKQERLQANDPHNQNLFSYLAEQAQRRQGQNTQASNCQAQPA"
     gene            complement(30639..31568)
                     /locus_tag="ACHOSM_RS12015"
                     /old_locus_tag="pgaptmp_002403"
     CDS             complement(30639..31568)
                     /locus_tag="ACHOSM_RS12015"
                     /old_locus_tag="pgaptmp_002403"
                     /inference="COORDINATES: protein motif:HMM:NF019371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HGU4558908.1"
                     /translation="MSRKPVPKVAFTKPPTSFSDQVRLLQQRGLVISDVPKAEFYLAQ
                     LNYYRFAAYCLPYEQDHASHTFITGTQFDQVLSLYVFDRELRLLVLDAIERFEVSLRT
                     QIAYQLSQKYQTAHPHLKPELFGDLLEYASSLGKLTGEVKCSREEFIKHLTQKYQEPL
                     PPIWACVELLTMGQLSCWYSNIKLRADRQVISKSYGLDEKTLTSFCEHLSLVRNLSAH
                     HSRLWNRDFIKTTVLPRHGEPALIQSLQNLPDSDRRLRKIYNTLVMLGYLMDVISGEH
                     HWKQRLIQLINHHQIDIDRMGFPADWQARPIWQ"
     gene            complement(31726..34620)
                     /locus_tag="ACHOSM_RS12020"
                     /old_locus_tag="pgaptmp_002404"
     CDS             complement(31726..34620)
                     /locus_tag="ACHOSM_RS12020"
                     /old_locus_tag="pgaptmp_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001568064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HGU4558909.1"
                     /translation="MVITDYQAKYFAQELTIRHAENGVDKLSQSLFDASVDLNPHQIH
                     AALFALNNPLSKGVVLADEVGLGKTIEAGLVLSQYWAERKRRILIICPASLRRQWAQE
                     LGDKFNLPTQVLDAKTWRISVNNGQTNPLANHCVSIMSYHYAARMETQLMLEPWDIVV
                     IDEAHKLRNAHRSSNKMGQTLRRALDGSKKLLLTATPLQNSLMELYGLSTIIDDQIFG
                     DERSFKQHFVNNESALGHLKKRLTGFIQRTLRKDVLEYVRYTQRQTLTVPFTPSNAEM
                     ALYESISALLEKDESYALPKSQRHLTGLILRKLLASSSHAVLNTLETIKRRLVDLKVQ
                     AKSDLDIISELVESDDLGEEYSEYVEETQSNTPESDIDFELLNAEIAELEHYIEQAKS
                     ITTDSKTQALLTALNQGFAKMEEMGAPRKVIIFTESKRTQEYLARFLEANGFADKLVT
                     FSGSNNHPEATKVYQQWLAENQGSDKVSGSPQIDRRTALIDYFRHHAEVMIATEAAAE
                     GVNLQFCSLLINYDLPWNPQRVEQRIGRCHRYGQQFDVVVINFLNEANKADQRVLELL
                     TEKFHLFDGVFGASDDVLGSIESGIDFEKRIQNIYETCRQPAEIEAAFDQLQKELEAD
                     INQKMRETQQLLLENFDEDIHDLLKIQLDAAQQRLDKVGRWFWQLSTHELAAIADIDP
                     SNHSFTLNQSINQAPTGSYQLVRRGQQSGSIETGKLANAHVYRITHPLGEWVISNALQ
                     RELPIASVIFDYSNHQTKISMLEPLVGLSGVLSLQKFSVESLERSEDHLLFAAQDEHG
                     HPLPSEVVQKLMQLSACVQSEVSEQELAALYPGYQYALQHALQQSRLGIEKQVNDRNL
                     SFFEQEVNKLDSWADDLKEGLEQSIKELDKQIKQVRREAKIAPTLEEKLALQKQQRQL
                     ESQRNKSRRELFDKQDEVDEKREQLIESLEGKLNKKTSQETLFTIGWTVQ"
     gene            complement(34607..34807)
                     /locus_tag="ACHOSM_RS12025"
                     /old_locus_tag="pgaptmp_002405"
     CDS             complement(34607..34807)
                     /locus_tag="ACHOSM_RS12025"
                     /old_locus_tag="pgaptmp_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4558910.1"
                     /translation="MTDTVLTIKEVAEYLKVNERTIYRLAAVHKIPAFKVGNAWRFKQ
                     SDLEEWISTQAAQGKGVLDGNN"
BASE COUNT         8976 a         8804 c         8362 g         9051 t
ORIGIN      
        1 aggaacgacc tcgctaatca gttaccagcg ctcacgctca aatatccaca ggaacggggc
       61 taaatcgcat tctggcgcaa tgttcactat cggggcaagc attcaccgga aaacgtcata
      121 caggcttaca ggcggcttaa aattctatgg gcggataaca gggcgggatc gggaggctta
      181 cgaaaatttc gtaacccccc tacacaaaaa gtgcaggtta ctacccgaaa agtgtagtgc
      241 cattgctggg ggctaaaaaa aaccgcagtg tgataacact acggcacgcc accaaagaaa
      301 cataacaagg aatcagcaat gagtaatacc tgtaagcctt cggggtgatt gtaacacatg
      361 agcaatcttt tactggaagc tgtttttcac tggatcacgt atcgttatgg ggattcccat
      421 atcggagagc atccaccagc ttcataacgg ataaccgaaa aatgataacg gttaacctgg
      481 aaaaaattcc ggtgaatatt tgccctgaca gttcccatat cggcggcaca cctgaaaaaa
      541 ccgtgtacac aaggatgtac acaataaaaa tcgttattac agaaatccca gtaatgacaa
      601 gcctttgatg cagatactca atgagcagat tcaagagtaa ctcgtaacac taattcttac
      661 ttttcagcct tcacaaatgc cgcccactca aacagagcgg catttttctt ccccgcaact
      721 tcaattctgt tttgcgtgcc tgctccagat tttgcgatgt ttttttgccc agcacactga
      781 gaacgtgaga tactcacaac tgtatataaa tacagttaca gatttacttt ctttgcaatc
      841 gatatcacat ggagtgggca atgaacgaac atcatcaacc ttttgaagaa atacggcatt
      901 atggcactga agggcaagaa ttttggtcag ctagagaatt ggcaccgtta ctggattatc
      961 gggactggcg caattttcaa aaggtactgg cccgcgcgac tcaagcgtgc gaagccagca
     1021 atcaagctgc atctgaccat ttcgttgaaa ccaccaaaat ggttgtatta ggttcagggg
     1081 ctcaacgaga acttgaagat gttcatctct cccgctatgc ctgttacctg gtagtacaaa
     1141 acggcgaccc tgcgaaaccg gttattgcgg cagggcaaac ttattttgct atccagaccc
     1201 gacggcagga gctggctgac gatgaagctt tcaggcaact tcgtgaagac gaaaagcgtc
     1261 tttttctgag aaatgaattg aaagaacata acaaacaatt ggttgaggca gcgcagcaag
     1321 ctggggtagc gacagctatc gatttcgcca tatttcagaa tcatggttac cgggggctgt
     1381 atggtggatt agatcagaaa gctattcatc agcggaaggg gctgaaaaag aatcagaaga
     1441 tcctggatca tatgggttca acagaactgg cggctaatct ctttcgagct acccaaacag
     1501 aagaaaaact caagcgggat ggcgtgaatt caaaacagca agcaaacacc acacactttg
     1561 acgtgggtcg caaggtgagg caaaccattc aggaacttgg cggaaccatg cctgaggagt
     1621 tgccgacccc gcaggtcagc atcaagcagt tggaaaacag tgtaaaaatt acagagaaga
     1681 aatagcagaa actaaaaact ctttttctta attgagatac ccgatgcgta agccaaagtc
     1741 caggtgcagt gaacacaatg gctaaacatt gcacctttct tttccacccc tcaattttaa
     1801 cctatttttt cttatgcatt ttctcagaca agaagtcaga ataatgcatc tacgctacag
     1861 aaaatagcga tttcacataa ctacaagtta tcaatttcct cttaccttaa aaaatctcaa
     1921 tcgtgacaat gcgcacaaat cgctaccctg ccagacagat ttttagggag agaaccatgc
     1981 tgttacacat tttgtatttg gttggcatca ctgccgaagc catgacgggg gcgctggcgg
     2041 ccggacgacg gcgcatggat acatttggcg taattattat tgcgaccgcc accgcaattg
     2101 gcggagggtc agtgcgcgat attctgctgg gccactatcc gctcggctgg gtcaaacacc
     2161 cggaatatgt gattatcgtc gctaccgccg cagtactcac cactatcgtt gctcctgtaa
     2221 tgccttacct gcgcaaagtg tttctggtgc tcgatgcgct cggactggtg gtcttttcta
     2281 tcatcggcgc acaggttgca ctggatatgg ggcacggccc aattattgcc gttgtcgcgg
     2341 cggtaaccac cggcgtgttt ggcggcgttt tgcgcgatat gttctgtaaa cgcatcccac
     2401 tggtattcca gaaagagttg tatgctggtg tctcatttgc ctccgccgtg ctgtacatcg
     2461 cgctgcaaca ctatgtttct aaccatgatg tggtgattat ctccaccctg gtattcggct
     2521 tttttgcccg cttactggcg ctacgtctta agctgggatt accggtcttt tactacagcc
     2581 acgaaggtca ctaaggttca aaacctgtga tctgctgggc agccagccaa ctgcccagct
     2641 tcttgatttg cgcattttcc ttccattcaa taacctgtct ggcgcgccca gatccagtcc
     2701 ccggcagctg ctgccagaac tgctccgtgc taaataaaag ttgcgaccag gaccgttcat
     2761 cactggcatt aagcgccgcc cgggttagcg gtattcccat tgccatcacc cagcgagtaa
     2821 aaggctgctt acgagccaga ttaaactgat gccataactg cgcactttta cttttcgcga
     2881 tccccggcgt gttctgtaat tgctctggcg ttaataaaag ccaggaaaag atatgctcaa
     2941 agcgatgagt ctgatgcagc gcgcgccaac cggcctcacc aatgccatcc agcccaagaa
     3001 cctgttttga ccccagccag actaagcgtg aaatgaactg ttcctgacaa acatcagaag
     3061 caaagtagca ggtcaacgag ttaaagcggt tttctggcgg tgtcggtttt gtacgttctg
     3121 caccgcgcca caccacatca tcaatgcgag gaataccctg accggcaagg ctgacgagaa
     3181 tctgatcacc aggcgcaata tcccactcct gccagcgcct gacggaacca atattcaccc
     3241 gctggacttt tttatcatcc agcatgacag gtgcgagtga cgcaaccacc gatattttac
     3301 cgatcttacc caccgcaaac tgaatcgcct tcacttcggc aacctgagct acaggttgat
     3361 atttccaggc caccagccac tctgcctggc ccggtagcca atggcgggat tctggctctt
     3421 tcgccgctcg tacaactacg ccatcggtga cgaagggtaa ttccgctttc caccactcat
     3481 tgcgtacgcg cgcaacttca tcagcatttt tcaccgcacg ggtatacgtc tgcgttagag
     3541 taaaacctgc ggtagccagc tcttttaaac gatcagacat taactgcggt ccatccggcc
     3601 atgcccagac aaaaacgccc agagaattca gcgtgtcgct atcgtcctgg cgcatcatca
     3661 agccagcaac ttttgcgcgg gcatttattc cccccatttg ttgttggata tgcccctcgc
     3721 gctggagaaa tatttccccc tgaagcgtac tgttggctaa aggcccgcta acggtttgcg
     3781 gcacagcgga aattaagcta actttctgcg tccagtcctc gcctttcagg ccattaccgc
     3841 gactgattgc tttgttcagt ttcccgtccc gataaaccag ggttaccgca acgccatcaa
     3901 cttttggctg cacccaaaga tcgctacgtt ctcgcatcca cagacttaat gcgtttttat
     3961 ccaccatttt gcgtacaccc gtatgggcaa ccggatgcat aaccgcacca tttaaaggcg
     4021 gcatcatcac gtcgcggggc tcgctcccaa agcagcgttg ccactgcgta agacgggcgc
     4081 ttaactgatc gtaaacaccg tcttccacct cactttttcc ttccttccag tagtcatcgt
     4141 cccactgttt tatttgctgt tgcaggcggg aaatttcttc ctgtgctctg gctggcgacc
     4201 aggccggaca gaccgcccac acagatgatt gccagcacaa gatacttatt aatatcgcca
     4261 tccatacttt catcatcacc tccgctgtag gtagtcaggc agatatacaa cgtgatgaaa
     4321 gcaaagccga gtggcaaaaa cggagtctgc gaggacgctt cctgaaaatc gtctttattg
     4381 cagtgaatga caggcaaatg cggaagcagc tacgcaaaac gcaacaactt tgcgcaaaaa
     4441 gtgtgagcaa gggctacgtc acatggccgc gccgtgtata ataagctcgt atgtaggctt
     4501 tatttcgcta atcacatacg aaagatactc atggctcaag gcacgcttta tattgtttct
     4561 gcccccagtg gcgcgggtaa atccagcctg attcaggctt tattaaaaac ccaaccgttg
     4621 tatgacaccc aggtttctgt ttcacacacc acacgccaac cgcgtcctgg tgaagtccac
     4681 ggtgaacatt atttctttgt taatcatgat gaatttaaag aaatgattag cagagatgcg
     4741 ttcctcgaac acgcagaagt ttttggtaat tactatggca cttcgcgtga ggccattgag
     4801 caagtactgg cgaccggtgt cgatgttttt ctcgatatcg actggcaggg cgcgcagcaa
     4861 attcgccaga agatgccgca cgcgcggagt atctttattt taccgccgtc caaaattgaa
     4921 ctggaccgcc gtctacgcgg tcgcggtcag gacagcgaag aggtcattgc aaagcgtatg
     4981 gcgcaagctg ttgcagaaat gagccattac gccgaatatg attatctgat tgtgaatgat
     5041 gacttcgata ccgcgttgac cgatttgaag accattattc gcgccgaacg tctgcgcatg
     5101 agccgccaaa agcagcgtca tgacgcttta atcagcaaat tgttggcaga ctgaacctga
     5161 tttcagtatc atgcccagtc atttcttcac ctgtggagct ttttaagtat ggcacgcgta
     5221 actgttcagg acgctgtaga gaaaattggt aaccgttttg acctggtact ggtcgccgcg
     5281 cgtcgcgctc gtcagatgca ggtaggcgga aaggatccgc tggtaccgga agaaaacgat
     5341 aaaaccactg taatcgcgct gcgcgaaatc gaagaaggtc tgatcaacaa ccagatcctc
     5401 gacgttcgcg aacgccagga acagcaagag caggaagccg ctgaattaca agccgttacc
     5461 gctattgctg aaggtcgtcg ttaatcacaa agcgggtcgc ccttgtatct gtttgaaagc
     5521 ctgaatcaac tgattcaaac ctacctgccg gaagaccaaa tcaagcgtct gcggcaggcg
     5581 tatctcgttg cacgtgatgc tcacgagggg caaacacgtt caagcggtga accctatatc
     5641 acgcacccgg tagcggttgc ctgcattctg gccgagatga aactcgacta tgaaacgctg
     5701 atggcggcgc tgctgcatga cgtgattgaa gatactcccg ccacctacca ggatatggaa
     5761 cagctttttg gtaaaagcgt cgccgagctg gtagaggggg tgtcgaaact tgataaactc
     5821 aagttccgcg ataagaaaga ggcgcaggcc gaaaactttc gcaagatgat tatggcgatg
     5881 gtgcaggata tccgcgtcat cctcatcaaa cttgccgacc gtacccacaa catgcgcacg
     5941 ctgggctcac ttcgcccgga caaacgtcgc cgcatcgccc gtgaaactct cgaaatttac
     6001 agcccgctgg cgcaccgttt aggtatccac cacattaaaa ccgaactcga agagctgggt
     6061 tttgaggcgc tgtatcccaa ccgttatcgc gtaatcaaag aagtggtgaa agccgcgcgc
     6121 ggcaaccgta aagagatgat ccagaagatt ctttctgaaa tcgaagggcg tttgcaggaa
     6181 gcgggaatac cgtgccgcgt cagtggtcgc gaaaagcatc tttattcgat ttactgcaaa
     6241 atggtgctca aagagcagcg ttttcactca atcatggaca tctacgcttt ccgcgtgatc
     6301 gtcaatgatt ctgacacctg ttatcgcgtg ctgggccaga tgcacagcct gtacaagccg
     6361 cgtccgggcc gcgtgaaaga ctatatcgcc attccaaaag cgaacggcta tcagtctttg
     6421 cacacctcga tgatcggccc gcacggtgtg ccggttgagg tccagatccg taccgaagat
     6481 atggaccaga tggcggagat gggtgttgcc gcgcactggg cttataaaga gcacggcgaa
     6541 accagtacta ccgcacaaat ccgcgcccag cgctggatgc aaagcctgct ggagctgcaa
     6601 cagagtgccg gtagctcgtt tgaatttatc gagagcgtta aatccgatct cttcccggat
     6661 gagatttacg ttttcacacc ggaagggcgc attgtcgagc tgcctgccgg tgcaacgccc
     6721 gtcgacttcg cttatgcggt gcataccgat atcggtcatg cctgcgtggg cgcacgcgtc
     6781 gaccgccagc cttacccgct gtcgcagccg cttactagcg gtcaaaccgt tgaaatcatt
     6841 accgcaccgg gcgctcgccc gaatgccgcg tggctgaact ttgtcgtcag ctcgaaagcg
     6901 cgcgccaaaa ttcgtcagtt gctgaaaaac ctcaagcgtg atgattccgt tagtctgggc
     6961 cgtcgtctgc tcaaccatgc gttgggtggc agccgtaagc tcaatgaaat cccgcaggaa
     7021 aatattcagc gcgagctgga tcgtatgaag ctggcaacgc ttgacgatct gctggcagaa
     7081 atcggacttg gtaacgcaat gagcgtggtg gtcgcgaaaa atctgcaaca tggggacgcc
     7141 tccattccac cggcaaccca aagtcacgga catctgccca ttaaaggtgc cgatggcgtg
     7201 ctgatcacct ttgcgaaatg ctgccgccct attcctggcg acccgattat cgcccacgtc
     7261 agccccggta aaggtctggt gatccaccat gaatcctgcc gtaacatccg tggctaccag
     7321 aaagagccag agaagtttat ggctgtggaa tgggataaag agacggcgca ggagttcatc
     7381 accgaaatca aggtggagat gttcaatcat cagggcgcgc tggcaaacct gacggcggca
     7441 attaacacca cgacttcgaa tattcaaagt ttgaatacgg aagagaaaga tggtcgcgtc
     7501 tacagcgcct ttattcgtct gaccgctcgt gaccgtgtgc atctggcgaa tatcatgcgc
     7561 aaaatccgcg tgatgccaga cgtgattaaa gtcacccgaa accgaaatta atgttttatg
     7621 aacccaacac gttatgcacg catctgcgaa atgctcgcca ggcggcagcc tgatctgacc
     7681 gtctgcatgg agcaggtcca caaacctcat aacgtttctg cgattattcg taccgcagat
     7741 gccgttggcg tacatgaagt tcacgccgtc tggcctggta gccgcatgcg taccatggcc
     7801 tctgcggcgg cgggtagcaa cagttgggtg caggtgaaaa cgcaccgcac cattggcgat
     7861 gccgtcgctc atctcaaagg ccagggcatg caaattctgg caacccatct ttctgataac
     7921 gctgtcgatt ttcgcgaaat tgattacact cgcccgacct gcattttgat gggtcaggag
     7981 aaaacgggca tcactgagga agcgttggcc ctggcggatc aggacatcat cattccgatg
     8041 atcggcatgg tgcagtcgct gaatgtttcc gttgcctcag ccctcattct ttacgaagcc
     8101 cagcgtcagc ggcaaaatgc aggcatgtac ctgcgtgaaa acagcatgtt gccggaagca
     8161 gagcaacaac gcctgttgtt tgaaggcggc tatccggtgc tggcgaaagt cgcaaaacgc
     8221 aaaggcctgc cttatcccca cgtcaatcag caaggcgaga tcgaagctga tgccgactgg
     8281 tgggctacta tgcaggctgc agggtaagtg ccatgaaagg tcgcctgtta gatgctgtcc
     8341 cactcaattc cctaacgggc gttggcgcag cacttagtaa caaactggcg aaaatcaacc
     8401 tgcataccgt gcaggatcta ctcttacacc ttcccctgcg ctacgaagat cgcacccatc
     8461 tctaccccat cggagaacta ctgccgggcg tttatgccac ggtggaaggc gaagtgctga
     8521 actgcaatat ctccttcggc ggtcggcgga tgatgacctg ccagatcagc gacggttccg
     8581 gcatcctcac catgcgcttt ttcaatttca gcgcggcaat gaaaaatagc ctggcggcgg
     8641 gccgccgtgt actggcttat ggcgaagcaa agcgcggtaa atatggtgcg gagatgatcc
     8701 acccggaata ccgcgtgcag ggcgatctca gcacgccaga attacaggaa acgctcacgc
     8761 cggtttatcc aacaacggaa ggcgtaaagc aggccacgct gcgtaaatta accgaccagg
     8821 cgctggatct gctcgacacc tgcgccattg aagaactcct gccgccggaa ctgtcacaag
     8881 taatgatgac gctaccggaa gcgttgcgca ctttgcaccg cccgccaccg acgctacagc
     8941 ttagcgatct ggaaaccggg cagcatccgg cgcaacgtcg tctgattctg gaagaactgc
     9001 tggcgcacaa cctcagcatg ttagccttac gtgccggagc acagcgtttt catgcccagc
     9061 cgctgagcgc caatgacacg ctgaaaaata aactcctcgc cgccttaccg ttcaagccaa
     9121 cgggcgcaca ggcacgcgta gtggcggaga tcgagcgcga tatggcgctg gatgtgccga
     9181 tgatgcgtct ggtgcagggc gatgtaggtt ccggtaaaac gctggtcgcc gccctcgccg
     9241 cgttgcgtgc gattgcccac ggcaaacagg tagcattgat ggcaccaacc gaattactcg
     9301 ccgagcagca cgccaataac ttccgcaact ggtttgcacc gctcggtatc gaagtgggct
     9361 ggctcgccgg taagcagaaa ggtaaagcac ggctggcaca gcaggaagcc atcgccagcg
     9421 gtcaggtgca gatgattgtc ggtacacacg ccatcttcca ggaacaggtg cagtttaacg
     9481 gcctggcgct ggtgattatc gacgaacagc atcgttttgg cgtacatcag cgtctggcat
     9541 tgtgggagaa aggccagcag cagggcttcc atccgcatca gttgatcatg accgccacgc
     9601 cgatcccccg cacgctggca atgactgcgt atgccgatct cgatacctcg gtgatagatg
     9661 agctgccgcc aggccgcacg ccagtgacta cggtcgctat tcctgatacc cgccgtaccg
     9721 acatcattga ccgcgtgcgc cacgcctgca taactgaagg tcgtcaggca tactgggttt
     9781 gtacgttgat tgaagagtcg gaattgctgg aagcgcaggc ggcggaagct acctgggaag
     9841 agttgaaact ggcgctacca gagttgaacg ttggcctggt acacgggcgg atgaaacctg
     9901 ccgagaaaca ggcggtgatg gcgtcgttta aacaaggtga gctacacctg ctggttgcca
     9961 caaccgttat tgaagtcggc gttgatgtgc ctaacgccag tctgatgatt atcgaaaacc
    10021 cggagcgtct gggtctggcg cagttacacc agcttcgcgg gcgtgtaggt cgtggtgcgg
    10081 tggcttctca ctgcgtgctg ctctacaaaa cgccgctctc caaaaccgcc caaattcggc
    10141 tgcaagtgct gcgcgacagc aacgacggtt ttgtgattgc acagaaagac ttagagattc
    10201 gcggaccggg tgaactgtta ggtacgcgtc agacgggtaa tgctgagttt aaagtggcgg
    10261 atttactgcg cgatcaggcg atgatcccgg aagttcagcg cctggcgcga catattcacg
    10321 aacgttaccc acaacaagca aaagccctga tagaacgctg gatgccggag acggaacgtt
    10381 actccaacgc atgaccagac tcatcacaag acaagtcacg cagtaaatct tcaatctgga
    10441 tagtgaggat atccattgcc tcatacgcat cctgtgcaat tcgcgccgcc attgcgcctt
    10501 ccggttcacc acgcagaatt tcagccgctg cctgtacgcc agccacaatc atttcaggtg
    10561 ttaacatttg gcgaagaaaa gcgcgattat cagaggacca gccatcttta tggcctaatg
    10621 gatgccccca gcctatccat tcttgcgacc ataacggatt ggcttctatg ggataaatta
    10681 gatgcgcggc atactgaata gcacacaccg cttttcctgg cgtatccagc gcccaccagc
    10741 gcgaccagag agaacgaatt aacggcgcgg ccccacccag agtattgaac accgccagcc
    10801 agcaaagaac gttattgaaa ccacgctcgt tatccatacg ctgcaacgcg gtgtccagta
    10861 aaaattgccg tacctgctgg cgtagggaag cattcatcat ctcctgccag agatagggcc
    10921 gacacaatgc cttcattaag gattcttcga agtgatcacc ataaccgcca gctaaaacgg
    10981 tttcatgcca ttgcgccagg cagagcggaa acagatagcg aaacaactct atttgcatcg
    11041 gttcgttata aacgagattg agtacatagt aagcactcaa atcccattcg ctgatctgct
    11101 gccactcttt agccgccagc gccttgagtt cggcatctgc gtaatcaaat tccgtttccc
    11161 atacacagag cactggcggc tcatcaccaa aaatatggcg acagtgttca cgtagcgttt
    11221 ccggttcctt ccgacgcttt ttcattttta accggcaaaa atcggcaaca ttaaatacag
    11281 cttaatcacc agcgcattga cgatatcgat aaagaacgca ccgaccatcg gcaccaccaa
    11341 aaacgccatg tgcgacgggc caaagcgttc agtgatcgcc tgcatgttgg cgattgccgt
    11401 tggcgttgca ccgaggccaa aaccacagtg acccgcagcc agcactgccg catcgtagtt
    11461 tttgcccatc atgcgccagg taacgaagat ggcatacaac gccatgaaga tggtctgtac
    11521 caccagaatc gccagcatcg gcagcgccag cgaagccagc tcccacagtt tcagccccat
    11581 caacgccatc gccaggaaca acgacaagct tacgttaccc agcacggata ccgcacgctc
    11641 aaagacgcgg taaaagccca ttattgacag accgttgctc agaatcacgc caacaaacag
    11701 tacacagacg aaggtcggca gttcaaaagc agtgccagcc aaaagttgcg caacaatttt
    11761 ccccaccgtc aggcagatag caatcagcgc gatagtttca atcagcacca acgaggtgat
    11821 catgcgtccc acatccggct tttcaaacgc cgtcgggact tcctggtcat ccggaatacc
    11881 gttcggcgtg gtggagtgct tcaccagata gcgcgccacc ggaccgccaa tcaagccgcc
    11941 cagcaccaga ccgaacgttg cacaggccat cgccacttcc gtcgcattgg tgaagccata
    12001 acgttcaatg aacaatttac tccacgcagc gcccgtaccg tgaccgccgg aaagcgtaat
    12061 agaaccggcc aacagcccca tcagcggatc aagccctaac aggctagcca taccaatgcc
    12121 aatggcattt tgcatcacca acagaccaac aaccacaatc aagaagatgc caaccacacg
    12181 cccaccggca cgcaaactgg caatgttggc gttcaggcca atggtggcga agaaagccag
    12241 cattaacgga tcgcgcaggg acatatcaaa gttgacttcc cagcccatgc tttttttcag
    12301 tactagtagc gccagcgcca ccaacaaacc acccgcaaca ggttccggta tggtgtattt
    12361 cttcaaaaag gagacggaat ggaccaactt acgcccgagc agcaacgtca gcgttgcggc
    12421 aacaagcgtt gctaaagtat cgagatgaaa catagttact cctttgtatc cgcatcactc
    12481 tttcatactc gtcatacttc atcgcgcagt ggcaacctca tgaactatga cgggtatcga
    12541 gttatgcttt cctgggtcaa attctcagct gttaatgaac aaatggcatg gattttaagc
    12601 agaaaaatac caaaagttat ataaaaaagt gcatttatct catttaaata tttagatgag
    12661 gatgtggcaa acgtttgctt ttctctttcg gtcagctaaa atgcccgctt tgctaccacg
    12721 ggattgtttt cgatgtctgt ttccaccctc gagtcagaaa atgcgcaacc ggttgcgcag
    12781 actcaaaaca gcgaactgat ttaccgtctt gaagatcgtc cgccgcttcc tcaaaccctg
    12841 tttgccgcct gtcagcattt gctggcgatg ttcgttgcgg tgatcacgcc agcgctatta
    12901 atctgccagg cgctgggttt gccggcacaa gacacgcaac acattattag tatgtcgctg
    12961 tttgcctccg gtgtggcatc gattattcaa attaaggcct ggggtccggt tggctccggg
    13021 ctgttgtcta ttcagggcac cagcttcaac tttgttgccc cgctgattat gggcggtacc
    13081 gcgctgaaaa ccggtggtgc tgatgttcct accatgatgg cggctttgtt cggcacgttg
    13141 atgctggcaa gttgcaccga gatggtgatc tcccgcgttc tgcatctggc gcgccgcatt
    13201 attacgccgc tggtttctgg cgttgtggtg atgattatcg gcctgtcgct aattcaggtt
    13261 gggttaacgt ccattggcgg cggttacgca gccatgagcg ataacacctt cggcgcaccg
    13321 aaaaatctgc tgctggcagg cgtggtctta gccttaatta tcctgcttaa ccgtcaacgt
    13381 aacccttact tacgcgtggc ctcactggtg attgcgatgg cggccggata tgcgctggcg
    13441 tggtttatgg gcatgttgcc agaaagcaac gaaccgatga cgcaagaact gattatggtg
    13501 ccaacgccac tctattacgg tcttggcatt gaatggagtc tgctgctgcc gctgatgctg
    13561 gtctttatga tcacttcgct ggaaaccatt ggcgatatca cggcgacctc tgacgtttcc
    13621 gaacagccag tgtccggtcc gctgtacatg aaacgcctga aaggcggcgt gctggcaaac
    13681 ggcctgaact cgtttgtttc ggcggtattt aacaccttcc cgaactcctg cttcgggcag
    13741 aacaacggag tgatccagtt aaccggtgtt gccagccgct atgtcggttt tgtcgtcgcg
    13801 ctgatgttga tcgtgctggg tctgttcccg gcagtgagcg gttttgtaca acacattcct
    13861 gaaccggttc tgggcggcgc aacgcttgta atgtttggca ccatcgccgc ctccggtgtg
    13921 cgtatcgttt ctcgtgagcc gctgaaccgt cgggcgattc tgattatcgc gctgtcgctg
    13981 gcggttggtc tgggcgtgtc tcagcagccg ctgattttgc agtttgcccc tgaatggctg
    14041 aaaaacctgc tctcctccgg gatcgccgcg ggcggtatta ctgccatcgt gctgaatctg
    14101 attttcccac cagaaaaaca gtaattcact ggcggcggta agataattac cgccgcagtc
    14161 tcacctcttt caccattccc gccttgagga ttgcgcgtaa atcgtgcata actcccttat
    14221 gtgcatttca cgggatggaa gaccatgaaa tttattggga agctgcttct ctacattctc
    14281 atcgctctgt tagtggtgat cgctggcctc tattttcttc tgcaaacccg ctggggagca
    14341 gaacatatca gcacatgggt ttccgagaat agcgactatc atctggcctt cggggcgatg
    14401 gatcaccgtt tttccgcgcc atctcatatc gtgctggaga acgtcacgtt tggtcgtgat
    14461 ggtcagcccg cgaccctggt ggcaaaaagt gtcgacattg cgctaagcag tcggcaactg
    14521 accgaaccac gccatgtcga taccatcctg ctggaaaacg ggacgctgaa tctcaccgac
    14581 cagaccgcgc cgctaccgtt caaagccgat cgtctgcaac tgcgtgatat ggcgtttaat
    14641 agcccgaata gcgaatggaa actgagcgcg cagcgggtaa atggcgacgt ggttccgtgg
    14701 tcaccagaag ccggtaaagt gctgggtacg aaggcgcaga ttcagtttag tgccggatcg
    14761 ctttcgctca atgatgttcc tgccaccaat gtactgattg aaggcagtat tgataacgat
    14821 cgcgttacgc tgactaacct gggtgccgac atcgcccgcg ggacattaac cggaaacgcg
    14881 cagcgtaacg ccgacggcag ctggcaagtg gaaaacctgc gcatggcgga tatacgtcta
    14941 caaagcgaaa aatcgctaac cgacttcttt gcgccattac gctctgtccc gtcgttgcag
    15001 attggtcgcc tggaagtgat cgatgctcgt ttgcaaggtc cggactgggc ggtgaccgac
    15061 ctcgatctca gcttgcgcaa catgaccttc agtaaagatg actggcagac acaagaaggc
    15121 aaactgtcga tgaacgctag cgagttcatt tatggttcgc tgcatttatt tgacccgatt
    15181 ataaacgcgg aattttcccc gcagggcgta gcgctgcgcc agttcaccag ccgctgggaa
    15241 gggggtatgg tcagaacgtc agggaactgg ctacgtgacg ggaaaacgtt gatccttgat
    15301 gatgcggcaa ttgccgggct ggaatatacc ttgccgaaaa actggcaaca gttgtggatg
    15361 gaaacgacac ccggttggtt aaacagcctg caactgaaga gatttagcgc cagccgcaat
    15421 ctgatcattg atatcgaccc tgacttcccg tggcagctca ccgcgctcga tggttacggt
    15481 gccaacctga cgctggttac cgatcataaa tggggcgtct ggagtggctc ggcgaatctg
    15541 aatgccgccg ccgcgacgtt taatcgtgtt gatatccgtc gtccgtcgct ggcgctaacc
    15601 gccaacagca gcacggtgaa tatcagcgaa ctgagtgcat ttactgaaaa aggcattctg
    15661 gaagccaccg ccagtgtttc acaaacgcca caacgtcaga cacatatcag cctgaatgga
    15721 cgcggtgtgc cggtgaatat tttgcaacag tggggatggc ctgaattacc gttgactggc
    15781 gacggcaata ttcagcttac cgccagtggc gatattcagg ccaatgtccc gttgaaacct
    15841 acggttagcg ggcaattgca tgccgtgaac gccgcaaagc agcaagtgac tcaaaccatg
    15901 aatgctggca tcgtttccag cggtgaagtt acatcgacgg agccggtgcg gtaaacatca
    15961 atctcgccgg gtacgactca gacgtacccg gcattccgtc aatagattac aacgtaattg
    16021 tcagcgcatt cccttgaggc gtcaccacca gcccctgctc actttcagcc tgcgaaccac
    16081 cttgcagacc atttactttc acaacattgc gcaagcacag tgtccagttc ttcgtctcgc
    16141 ccgcaccagt cacagtaatc gtgttgccag tacgtactgc ttttaaagta aagatcaccg
    16201 atccgtcagc agcgggcact tcacagacgg cttcatgccc gtcttgcaga ttaaagaggt
    16261 ggaatgccgt gccttcgtgc cacgcgtaat cgggacgttg atcgttgttg cccagcgcca
    16321 gtagagtgtt atcacgcaca taaacgggca gactcaggaa gccgtgctgc tgtttatgcc
    16381 agcgactacc gtcgagttca tcgttgtgcc acaggtgtgt ccagcgacct tccggcaggt
    16441 agaactgcac atcgcccgct tcagtgaaca ccggcgcaac catcacgttg tcgcctaaca
    16501 tgtattgacg gtcaaggtaa tcacaagccg gatcgtccgg gaactccatc atcatggccc
    16561 gcatcatcgg cgtaccccgc gcgttcgcac gcgcagcttc acgatacaga tacggcatca
    16621 tgcggcattt cagttgcgtg aagaagcgca ccacatcaca ggactcatca tcgtaggccc
    16681 acggcacacg ataagatttg ctaccgtgta aacggctatg gctggagagc aaaccaaacg
    16741 cgcaccagcg tttgtaaacg tgcgccggag cggtattttc aaagccgccg atatcgtggc
    16801 tccagaagcc aaaacctgaa aggccaatag acaaaccacc gcgcaggctt tccgccattg
    16861 attcgtagtt agcgtaacaa tcgccacccc agtgtaccgg gaatttctgc gcaccgacgg
    16921 aggccgagcg ggcaaacaag acagcttctt cctcaccaac ggtgtccttg agcacgttcc
    16981 acaccagttc gttgtagatg tacgcataat ggttatgcat tttctgcgga tcggaaccgt
    17041 caaaccactg aacatcagtt gggatacgtt cgccaaagtc ggtcttaaag caatcaacgc
    17101 ccatcgcgac cagacctttc agtttgtcgg cgtaccattt gcaggcatcc ggattggtaa
    17161 agtcataaat cgccagacct ggctgccatt tatcccactg ccatagcgaa ccgtccgggc
    17221 gtttgagtaa atagcctttc tcttgtaact ctttaaagac gggggatttt tgaccgatat
    17281 aggggttaat ccagacgcag attttcagcc ctttcgcttt caggcggcgg atcatccctt
    17341 ccgggtcagg gaaagtcagc gggtcccact caaaatcgca ccactggaag gctttcatcc
    17401 agaaacagtc aaagtggaaa acatgcagcg gcagattgcg ttccgccata ccatcgataa
    17461 agctgtttac cgtcgcttcg tcgtagttgg tggtaaatga agtggttagc cacaggccga
    17521 aggaccacgc gggcggcagc gccggacgac cagtaaagcg ggtataacga tcaagtaccg
    17581 ctttcggcgt cgggccgtcg ataacaaagt attcgagata ttcactctca acgctgaact
    17641 gcactttgga gactttctcc gatcccactt caaaagagac acactgggga tgattgacca
    17701 gtaccccata accacggtta gtcatgtaga acgggatatt tttatacgcc tgttcagtac
    17761 ttgtgccgcc gtcccggttc caggtctcta ccgtctggcc attgcgcacc agggcagtaa
    17821 agcgctctcc cagaccgtaa actgtttcgc caacgccaag atcaagccgc tcaaacatat
    17881 aattgcgttg attattcgtg tcctgcacgt agccattatt tttcacctga ctaccggtaa
    17941 tacgttcgcc gttgcgcaga aaatccagtg accagaactc acctttgctg acacgcgcgc
    18001 ttaagttgcc acttttaaac tcagcataac gttctgtgtt ttcgattgtg accttcacgt
    18061 cctgcaaaat attgagcgga taatgaggac cgttattcag cgccccctga aaatgctcaa
    18121 tccgcacacc gacaatacct tcctgtgggg agaaaaagcg cagcgtaaat aaaggcgtat
    18181 caagctgcca ggtacgttca cgcacatcac ggggggcagc atagaccacc atttcattat
    18241 cctgctgttc aacctcgaac acctgaagcg ggtgaatcaa attgaggcca ggttgaatca
    18301 accagtttcc atcgctaatt ttcattgccg ttccttagtt ctgcacttct tgagaggtga
    18361 attgttgctg gcaacggcgt ttaccctgag ccagctgttc cataacggtt ttcagattgt
    18421 gcgtcgtgag tgagtagtag cgtttagcga taatcgcgct cagcaaataa cagatcgccg
    18481 gaacaatcgt gaatagcgca ataatgatgc taatcgtggc gctgttctgc gctttttctg
    18541 ccgcatcata tccgccataa gccagcatcc agccgataag agcgccgccg aaggccaacc
    18601 ccagtttgag cacaaacagc gtgccagcaa aactgatccc ggtcaggcgt ttaccattgc
    18661 accactcgcc gtagtcgacg gtatcggaca tcattaccca ctggataggt gtcaccagtt
    18721 gatgcaacac accaatcacg aagatgaaga caaacatagt gatgctggcc tgcatgggaa
    18781 caaagaacat cgcgaggcta atcactgcca gcagggcgtt cgtccaccag aagatagtga
    18841 ctttacattt ccagtcggtc agaggttttg ccagtgcgga accaatcagg ttacccacgc
    18901 aataagtggt gagaaaagcg acaaacactt ccggcgtgcc caaaatccat gtgacgtaat
    18961 acatcatcgc cccaccgcgt acgcacaccg ccaggatatt gaaaatggtt agtaaaccga
    19021 caatccgcca ctggtcgttt tgccagatat cacgtaaatc ttcccgcata gacgttgttg
    19081 taggtggtgc ttcaacgcgt tctttagtgg tgaagaaaca aaatgccagc atcatgaatg
    19141 ccaccacgga aaggaccgcg ataccgccct ggaaaccgag tggtttatta tcaccgccaa
    19201 ttaaattaac cagtggcatc atcagaacag tagaaagcat gcctcccgcg gtcgccagca
    19261 caaaacgcca ggattgcagc gagatacgct gagtcgggtc attggtgatt acaccaccca
    19321 atgcgcagta agggatattg acgacggtat aaagtaaggt aagtagggtg taagtaattg
    19381 ctgcatagat cattttgccg ttcatactga gatctggcgt gctataggcc agtacacaga
    19441 cgatcccgaa tggcagtgcg ccaaacagta cccacggacg aaatttaccc cagcgagagc
    19501 gcgttcgatc ggccaacaac cccatgcaag gatcggaaat cgcatccagt gcacgagcga
    19561 ccaaaaacat ggttccgaca aatccggcag gaatgccaaa aatatcggta taaaagaaca
    19621 tcatatataa cattacgtta tcgaaaataa tgtggctggc ggcgtctccc atgccataac
    19681 caattttctc tttaacggac aacacttcac tcttcatctg cttttccttg atgctatagg
    19741 ggtgctgaga ccgataacgc attttgtaaa ccatccctgt gagagcgaat attccgatat
    19801 ctggttatta atttatgatt cttgttttat gtgatcgtgg tagcgttaat tccgctcata
    19861 tatcattgta aaatatgggt tttatatgaa ccataatgct ttcgtgataa tacgctgcgt
    19921 gtattaggcg gaaaaaactg atctggggga tgtagaaact caaggaagta gctataatgc
    19981 gccccgcctc catgtagcaa tcgaggcgcg gaagatcgtc gtctccggtg aggcggctgg
    20041 acttcaaatc cagttggggc cgccagcggt cccgggcagg ttcgactcct gtgatcttcc
    20101 gccaattaac atcttctgaa ctctcccgat gtcaatcccc ccttctaatt ccttgtatat
    20161 caggattctt ttgtatccag aagtcaaccg acttcactcc gcatcaagtt gcttttgggg
    20221 gtactttagg gggtattatt tcctattgaa atatcagcac ccccataatt ggtaaaatat
    20281 gatatataac aacggctttc aacaacatct ttgttataca aggggggaca ttgggggtat
    20341 caggttaatt actggcagaa atccccctca ggaacatgaa ttcattaaaa gccggaggat
    20401 gtcatggcat taaccgatat taaagtgaaa accgcaaaac caaaagaaaa gccctataaa
    20461 cttgctgatg gcggcggtat gtatctactg attaacacta atggttctaa atactggcgt
    20521 atgaagtacc gatttgctgg taaagaaaag atgctttcta ttggcgtata ccccgatgta
    20581 acgctagccg acgctcgtga aaaacgcagc gaagctagga aaatcctcgc tgctggaggc
    20641 gatccaggga tggcaaaaaa agaggaaaaa atcgcgcagc aaatgagttt gaaaaatacg
    20701 ttcgaggcca ttgctcgcga atggcatcag tcaaaagccg atcggtggtc attacgctat
    20761 cgtgatgaaa tcattgatac ctttgaaaaa gacatttttc cctacattgg caaacgccct
    20821 atcgccgaaa tcaaaccaat ggagttactg gaagcactgc gtaaaatgga aaaacgtgga
    20881 gctctggaga aaatgcgcaa agtccgccag cgctgtggtg aggtgttccg ctatgcaatt
    20941 gttactggtc gggcagacta caaccctgct cccgatcttg cctgtgcttt agctacaccg
    21001 aaaaaagtac attttccctt tcttactgct aatgaacttc cccacttcct caacgatctg
    21061 gctggttata ccggaagtat catcactaag acagctactc agatcattat gttaaccggt
    21121 gtacgaacac aagaattacg tttcgcacgc tgggaggata tcgattttga gacaaaatta
    21181 tgggagatcc cagcagaagt aatgaagatg aagcgacctc atattgtgcc gctctctgag
    21241 caggtcattg cgctatttaa gcaacttgaa ccaatctcaa aacatcatcc tctggtcttt
    21301 atcggtagga acgatcctcg caagccaatt agtaaggaaa gcattaacca agtcattgaa
    21361 ttgctgggat ataagggaag actcacaggg cacggtttca gacacaccat gagtacaatt
    21421 ctgcacgaac agggttttaa ttctgcctgg attgaaattc aattagctca tgtggataaa
    21481 aacgctatca gaggtaccta taatcatgcc cagtatctgg atggtcgccg tgaaatgatg
    21541 cagtggtatg ctgactacat tgattcactt tctgagcaga tctgatgaat gaaggttcag
    21601 tgacacaaaa ataatgaata accatccggc cccaaaagcc gtatggtttt atttatgttt
    21661 tcttttttat ttgctcacac cgcctgaact ctcattgggg aaccaagatg cgaacaccac
    21721 atcttgtgcc actcagcaaa caggctgtag ccatactgac agaacttcag acatgggctg
    21781 gtgaaaatgg tctgatcttt acaggagcac atgacccgcg taaaccaatc agtgaaaata
    21841 cagtcaataa agccttacgt gtgatgggct atgatacgaa gactgaaatt tgtggacacg
    21901 gttttagaac gatggcgtgt agctcattag ttgaatcagg tttatggtcg aaggatgcag
    21961 tagaaaggca aatgagccac caagaacgta actcagtgag ggctgcttat attcataagg
    22021 cagaacatat agatcaacgc cgattaatgg tgcagtggtg ggcggattat ttggatgcta
    22081 atcgtgaaca aacgatttcg ccattcgact ttgcaaagtt gaataatcca atgaatcact
    22141 aaaactttag ccatgtcata taggagatcc attttcaata cgaggagtcg aatactacgg
    22201 tagtatgccg actatatcga ttcattgaga aatggtggaa atattcttca tgggaatttt
    22261 aatcgccaaa aagcatagtc attaatcact atatcgtaat atgtcttatt caatatctat
    22321 acaccactaa aagcccctgc cctcacggac tgaggctttt ttgcttttta atcaaaataa
    22381 aaggaaatca tgatggaaaa ttacatttgt tgccctgtat gtgactcaac ccgtattcgt
    22441 gaacgtcata tagggaaaag acaggagctg ttgctggtgg cgtatcaggc gcactagctg
    22501 gagcgtctat cggctctgct gttcctgttg tagggacgat tgctggtgct gcggtaggcg
    22561 ctctatttgg acgtttagta gctggagcta ctgctggggc tgtgagtggt tcagctttag
    22621 atggcgtggt atttgatcgt tatgaatgta tcgagtgtga atacacgttt gattgatagc
    22681 cataagttaa cggaaacccc tgatggggtt tcatctctta ggagtctgtg agtctgaact
    22741 gaccctagtt tattggacag aggctttttt cgttttaacc cgcgcaaatt gagatcatga
    22801 tagaaagcat aacatgttat cgggattgta aggcaactca ttatcctgct ctaaaccatc
    22861 attaatcatt tttaaagcga gttgcctgtt tttcagctga ttggcttttt atatcaatat
    22921 catcttctaa agggaaatag atccctttat caaaatatcg ccattaatta aatcacaata
    22981 catcaataat aggacatgaa tatgcgtata actgcgacgg cttccccatg ccgaaaatcg
    23041 gggatgattt ctgtttttat tacgaactta ggaaaatata acgaaggtga actggttggt
    23101 gagtggcgat tcaaaacagg gaaattagcc cattcactgt ttggcttctc gattgaaatc
    23161 caacgctgct aagccttgcc agtactggat aaaagcaagg tttggcactt tttgcgcctt
    23221 aaaccaactt cgagcccaaa gtacaaaccg tttgattttt taaacccaaa atgcaaacga
    23281 tcgtttggcg tctcgatttt tacacttggc gaatgccgcc aaacagcacc gtattggact
    23341 atgtatatca aatagttagc cgcgcacagg tcatcataaa aaaccgattt gactggttag
    23401 tttcatggcc atttctttca tctatttcat caccgagtct gcacccgacg actgaaaata
    23461 gacggcaaga cgaaccaaca aggtgataag tgacgtgtta tgggcaagtg gcaacgattg
    23521 ggatagagaa tggatacagc aggtggtcgc tttactggtt gggcatggtg cgtttactct
    23581 ctacccagca ttaagccagt gcgataatgc ggccgaacgc ttaacccgct ggttgaccta
    23641 tcagttacca cgcgttaaac aagcgcaccc aggctgcccg attcaccaat ggctaaatag
    23701 cattggcgca caagtgatcg tagaaaaatg gcaaagcccc gagcaatggc caaacctgta
    23761 ttggctacct ttacctgatg ttgatggcaa cgcgcaaggc gggttagctt tgttaccagc
    23821 gcatttatta ccggcagatt ttttatcggt atatgtggat ggcacgattc aaaccagaac
    23881 attgcaagca aacacaccgg ccaacacctg tattttttgg tgcatcacac ctttatctaa
    23941 aacaggaaag cgcgctacct tgcaccccaa acagcaaaac cgagcttttt gcctgcccag
    24001 ctcgccggag caaatgggta aatcgtgcac aggtttatta ctgagcggtt taagaaaaga
    24061 caatggaaaa tgaaacggca acgtgatcag ccaccagatc aacctagcgc tgctgcacca
    24121 tctttgtaga ccggagaatg atgtttggat gaaagcgagt tttggtaccc tcatcattga
    24181 gggtaccaac agcaaaatac tcggcgaatg ctattgtcta agcacagcgc caagccttag
    24241 cggcacgaat agttgttaac cttactcagc tttagcaaac tgctgggtga agtaagccaa
    24301 tgaattagct tcttttacgg catcatgcgg aatgagtaag tatttccaag gtttgccacc
    24361 gtgctgtgca aggtattcac tggcatgact acaccatttc acaccactgc gggctttttc
    24421 ggtgacttcc tcattccaac tgccgtcttg gttttgatgt tgagtacttt tagtttcaat
    24481 catcaataca tagtcggcgg tttcagcaac gaaatccggc acgtattcag gatgctcgct
    24541 accgcgtttg tagtacatct tgaattggcc tttggcaggc ttaaaccact tctcgcagtc
    24601 gcgttctaaa atcaccgcaa aacggcgctc agtgtctgaa tcaaactttt gcaatgggta
    24661 tagacaacgg ctaaagccgc caaagagcat ttgtttgatg cgactgacat cgcgtactgt
    24721 atcacggtag ctttgcaccg gctggtcttg cgctatggtg taagcgcagt cacgtaaggt
    24781 actaaagcca ctgcgtacat cgacgacgta ctcggttgct tcttcaaaga agtggttgtt
    24841 cagttgcgca tgaattaact tcgcaatcgc atcactctgg ttttgcagaa tgttctcaac
    24901 cacatcgtcg tcgttttcag cggccagtgt attgacagct tgagtggcta ggtcaaacaa
    24961 aagttcagct tgctcgtcgt aaggaatatc gtcgtaatcg aacagcttct tcaaaatata
    25021 gttttgtttc accggctcgt agttgcccga atcgccttcg ccacgaatgg tttggtgatt
    25081 gtcttgcagg ctttgttgaa tcagagttcg gtcgataggt tttaaacctt taatggcagc
    25141 aatgtccaaa tcgaacgggt gatagccggt acgtacttca cctgatggtg aaacaataat
    25201 acgcggaata tctatggtgt tatccaccgt gagctgggtg acttgctcaa caatcgccgc
    25261 aatatccacc gattcttcag taaatagctc ccgttgctct tgggttaact gagcttttac
    25321 ttgttcggtg atctgtgctt ttacctcagt cgttaacaat gctttgctgg tcggcgcatc
    25381 ggtagggcgt gtttgatatt gcttgatcac gtccaatgca gcgcgggcaa cggtttgttg
    25441 gttaggcgtg gtataagtgg ctttaggttc ttgtacctca cggttaccgt ctgcaccggt
    25501 tgcggttaca cctgaaccag tcagttgctg ctctaggttc gatttaacct gcacactttg
    25561 tttggttaca tcgtcttctg ggcgatctaa tacgcgtgtt ttcaggcgca gtgggttatt
    25621 cgggtcgtta gcctcatcaa caatttcttg gaatttgtcg tgcgcaacaa tgctcagcgt
    25681 atcgacggct tcaacacctg tacgtttgcc ataaggtaaa cgcaaaccac ggccaattga
    25741 ctgttcaatt agggttctgg cgttggctgc acgtagtggc acaatggtgt acaggttagt
    25801 aacgtcccaa ccttctttca gcatgttaac gtgaatgacg atctcggttg gctcatcgac
    25861 atgctcaaca ttgagcagtg cttgaatagt ttcttcttct tttttactgc tatccacttg
    25921 aatgactttg ccacggtagc ggccgtcaaa cactgtttca agtttggtta aaagctctgc
    25981 tgcatgcgtg gtatcacggg caatcaccaa cataaaaggc ttaactaact tagcatcgtt
    26041 ttcatgtgca taagtgatta aatccacctt ggtttgctca tgtaaacgaa taccgtccat
    26101 taacttaatg gtttctagtt cgtctttatc atattggctg gcatcaaagt tacgctgagt
    26161 aacaacggca ggctctttaa caaaaccatc atccatggca caggccaatg ggtaatcaat
    26221 caccacattt ttaaagtgca cagggccacg acttgattcg gtaaatggtg tggccgttag
    26281 ctctaatgcg aatagtggat taagctcatt gagcgcacgc ataccggcat cggcacgata
    26341 gcgatgcgat tcgtccatca gcagcactaa gtcaggtaag ttcgatagat actcaaagta
    26401 gctttcgcct agggtttccg ctaaacgctt aatacgcggg gctttgcctc cacgtacttc
    26461 ggtgttaatt ttcgcgatgt taaacacatt gatccgaacc gaaccaaata gatcgcccat
    26521 gacttgtgcg ccgcggctct catagttatc accggtgatc acttctggcg gtgctagcac
    26581 aaaatcggta atgcctttaa acacgtactt cggtgaggcg ggcgagaaat cgctgatcag
    26641 cttttcataa atagtcaggt taggtgccaa cacaaaataa ttgcgaatgc catgtacctg
    26701 atacaaatag gtaataaacg cccccatcaa acgggtttta cctacacctg ttgccaaggc
    26761 aaaacacagt gaaggaaagt cacgttcaaa atcgctgagc ttaggaaact gcgcttttaa
    26821 ggtttctaat agctccggca catcgcgttt tgggttcagt agtttgtgca cagaagcact
    26881 aatcgccact gccaacgcac gcagcgaatc ttgctgcggc tggcgtaagc ttaaacggcc
    26941 aataatatta ttaatggcac gtaattggtc gggttttaag cttgataaat ccatggtcat
    27001 tataattatc cttattaacc gttaatcttg cgctgcatcg tcatcggcag acgcatcgtc
    27061 agttaggttt gccacgttta acgaataatc gtcctgacca aattggcact tgtctaaaat
    27121 ggcttttggc agttttttca aggttaaatt ggtaaaacga cctggctcgc agcgaaatgc
    27181 accacacata accagtaaag ttcggtcact gccaacttct tctgacagcg cttctaattt
    27241 atctaccgat aagctttgtg tggtgacata aatatgatcc gtctcggttg aataaccgtg
    27301 atcccaccaa ttcacttccg atggcgcata ggtaaaacct tccagcttac aaacggcttc
    27361 ggcgagcatt tctgggttgt actctttatt gatcacttcc aagccaaact tatctttcac
    27421 aattaaggtc ggggcaagtt tgtaatagcg gaagccaccg ccaccttgcc agtttacaga
    27481 cttagaaata ccaccttgat cttcgccatc aatcactttt tgcaagcgcg ggataatatg
    27541 ggtatgacaa tgatcaccta gctcgaccga gatccaacgt cttttcatct tgtgagctac
    27601 agcagcagta gttcccgaac caccgaatga atcaaagact aaatcgcctt ctcgcgtaca
    27661 catttcgaaa atccgcttca aaagcgcttc gggttttttt ccatttttga aactaacgcc
    27721 gccttcattt gctattccat tccaactaat gtcagtccaa acatcagtta ttagttcagc
    27781 aggttgcatc tcaccgtcaa tttccaaaag cctattggag taaaagacca tttgcctacc
    27841 atttgcgata taaaagcctt cgacatcctc atttggatgt acataaacct tatccctatc
    27901 tttctttgac ttctcgatag tttcttttct ctttatttta gcaccaccac caattgcaac
    27961 ggattgaaat acacgttctg cattatcaat tgcgaattgt gctaattcat catcaaatgt
    28021 atcaggcagt tttttcttgg cttcttttgt atctttatag ccattggcct ttgcaaacgc
    28081 atcggcaata ccgcaccacg tccaactttt ataatggtca tctgggttta atagatattt
    28141 gttatatccc gtatcatatc ctttaggtat atagattttg ttaagcggtt tctgggtgcg
    28201 atcttttgcg tatacaagca aaaagtttac tgtggagaaa actgtggctc ctgttgcctt
    28261 aaagcctgaa gggtcatttg tggtcatcga tatggtattt aactgatttg cacgaccaaa
    28321 aatctcgtcc atcaaaactt ttaaataagc catttcgcag tcatctaagt gaacgaatat
    28381 tactccatca tcacttagta gattacgaat tatttctaac ctatccctca ttaagcccag
    28441 ccaaatggaa tgctccagac catcatcata gtgctcaaaa gcacttccgg tattgaatgg
    28501 tgggtctatg taaacacact tcactttgcc tgcatattct tgctctagcg ccttcaatgc
    28561 caataagtta tcgccaaaaa tcagtttatt atcaaaaata tcgttttcgg tcactttttg
    28621 gctggcgtga taagacttgc tcgggtcttc taacaaaatg cgcggctcta actttttgcg
    28681 ctcgtttttg cctatccagg tcagttctaa tttggttttg ctcatcattt ctgtgctctt
    28741 atcttgtgtt cgttacttag cgttattatt ctacagcaat cgctacgcag gttgtgcttg
    28801 gcaattactc gcttgggtgt tttgcccttg tcggcgttgc gcttgctcgg ctaagtaact
    28861 gaataaattc tggttgtgtg ggtcgttcgc ttgaaggcgt tcttgttttg ctacaactgc
    28921 cgggctttct gccaagttta aatggctaat ccacaccgtt tgcattaaac gtgcggtatc
    28981 gcccaagcgt ttgaagtatg cgtgcggcgc actgcctaat actaatgccg taaaactatc
    29041 aaaaaattgc tgacgaagtt catcaaaagc gtggctgatt aatcttttaa atgccgcttg
    29101 ctcggtaatc tcaggagccg taatagcgcc ttcaaactgc gccattaata gcgtggttat
    29161 gcagtaagta aaatgtcgat ataccagttt ttctgggctt ggactactgc gctctgcgtt
    29221 tgtacataag acttgaatag ccagatagca gttcaccgcg ttaatggcga cggccccctg
    29281 taaatctgta ctaaaaacgg cttggtactc ggcactttct tggttagtga atttgcttgg
    29341 ctctactttt agctgataag gcacgcgtac atcaccttgc atacaggcca atgctttggc
    29401 caaggtgtcg atagtaatgc tgcgaatacc cgctgctgcg ctttgttgtg ggcggtagag
    29461 atattcaaca ccaaacattt tcatctcttg gcgcaagcgt tcttgggtat catccagcgc
    29521 ggcaaagttg gctaaatcga ccgggttttg caggttacgc gctttggtta cttgtttgtg
    29581 aaatgcatcg ttacccgtgt tgataatggt taccatcact tgagcttggc tgatgtctgc
    29641 atttgggttt tcacgcataa actgtgcagc actagcaatg gtttgtgcac cgttaacgac
    29701 ggacaggcca tcgactgcaa catgcttgct acctgtgtgg cctctaatat tcccttttgg
    29761 ttttactgca ttgccaacca gagtaatgcc attgttaaga taaataaaat tttccggctc
    29821 atgtaaaagg gtgtctttaa tcgccctatt taccccacgt ctgccagcac caataaaata
    29881 gcgaatattt ttctcgtaaa gtttacggtc atgagtagtg tgcaaggcaa ttaaatcggt
    29941 tagcttggcg ataccaaata cgacttttcg tggttgttct tgcactcgga acttatatac
    30001 agcgacacgc tcgtttaccg tatcaaccgc atgttcactg cgaagagcct gctcaacatc
    30061 ggtggcggtg tattcttgat attcaggtgc gatttgctct tcaccttcat caatcagctc
    30121 ttggatcgct ggttgtaggt agttttgtgc ttgtatggta atgccattac ctgtgtatgc
    30181 gacaagcagt tgaatgcttt cgcactcgtc taatgcggct tcaatatttg cttgaaggtt
    30241 ttgaacattt tgattaaagc ggtgaaattg cttgtttaaa agtaacttta caccctcaat
    30301 aaaagcctgt gcttcgccca attggaattg cgcatcttcc ttcatttttg actgtactaa
    30361 gtacagagtc tgatcagctt cgtgataata aattgcatca ataccatggt cttcatagtc
    30421 gtccaccaca gcttttgctg cggtcactac gtctaaatcg aatttttgct gtagtacgaa
    30481 tgcgctaaaa gccctggaaa tttgcttttt aggtttatct tctcgcccac cttgcccaag
    30541 caatggcggt aaatgcggca caaaacgctg ctgtagcagt tcttttaatg gggtaagaaa
    30601 ttgtggttgt aatgctgcca agtgagttgt ccttattttt attgccaaat tggccgcgct
    30661 tgccagtcgg ctggaaagcc catgcggtca atgtctattt ggtgatgatt tatcaattgg
    30721 atgagtcgtt gcttccagtg atgttcacca ctgatcacat ccatgaggta gcccaacatc
    30781 accaaggtgt tataaatttt tcgtaaacgc cggtcgctat cgggcaggtt ttgcaagctt
    30841 tgaatcagtg ccggttcacc atggcgtggc agtaccgtgg ttttaataaa atcacggttc
    30901 catagcctag aatgatgggc acttaaattg cgcactagcg acaaatgctc acagaatgag
    30961 gtaagagttt tttcatctag gccgtagctt ttgcttatta cctggcggtc ggcacgcagc
    31021 ttgatattgc tgtaccaaca ggaaagttgc cccatggtta acagctcaac acaagcccaa
    31081 atagggggta gtggctcttg atatttttgc gtaaggtgct tgatgaactc ttcacgactg
    31141 cactttacct cacccgtgag tttgccaagg ctactggcgt attctaataa atcaccaaag
    31201 agctctggtt ttaaatgagg gtgcgcggtt tggtattttt ggctcaactg atacgcaatt
    31261 tgtgtgcgta atgacacctc aaaacgctcg atggcatcta gcactaacaa acgtagttca
    31321 cggtcgaaaa catataggct taggacttgg tcaaactgag tacctgtgat aaacgtatga
    31381 ctggcatgat cttgctcata gggcaaacaa taagccgcaa aacgatagta gttcagctgt
    31441 gctaagtaaa actcagcttt cgggacatca ctgatcacta ggccacgttg ttgcaacaag
    31501 cgtacttgat cgctaaagct tgtagggggc ttggtaaaag cgactttagg gactggtttg
    31561 cggctcattt gacctcccat gcaacagaag atcgaatttt gcgggcccca gaaacgagta
    31621 acccctcaca gggcgctgat ctgacgggta gcgggagggg tgttgttgca gtgatcatag
    31681 ccccaaactg aaaaaagtca atgctggcgt tgaaattgat tgtcatcatt gaaccgtcca
    31741 cccaatggta aatagggtct cttggctggt ttttttattc agtttgcctt ctaagctttc
    31801 aatcagctgt tcgcgctttt cgtccacttc atcttgttta tcaaacagtt cacggcggct
    31861 tttattgcgc tgactttcta actggcgctg ctgtttttgc agcgcaagtt tctcttccag
    31921 ggttggagct attttggctt cgcggcgtac ttgcttgatt tgcttatcaa gctcttttat
    31981 agattgttca agcccttctt ttaaatcgtc tgcccaagaa tctaacttgt ttacctcttg
    32041 ctcgaagaag ctgagattac gatcgttaac ttgcttttcg atgcctagtc tcgactgctg
    32101 taacgcatgc tgcagcgcat attgatagcc aggatataag gcggcaagtt cttgctcact
    32161 tacttcactt tgcacacagg cactaagctg catcagcttt tgcacgactt cacttggtag
    32221 cggatgacca tgttcatctt gcgcggcgaa caataaatgg tcttcgctac gctccaatga
    32281 ttcgacacta aatttttgta ggcttagtac acctgacaaa cccactaagg gttctagcat
    32341 actgattttt gtttggtggt tgctgtaatc aaaaattacg ctggcaatgg gtaattcacg
    32401 ttgaagcgcg ttgctgatca cccattcacc cagcgggtgg gtgatacgat acacatgggc
    32461 attggctagt ttaccggttt caatactgcc gctttgctgt cctcggcgta caagttggta
    32521 gctacctgta ggagcctgat tgatactttg attaagcgta aaactatggt tgcttgggtc
    32581 aatgtctgcg atagctgcaa gttcatgcgt actgagttgc cagaaccaac ggcctacttt
    32641 atcgaggcgt tgctgcgcgg catctagctg aattttgagt aagtcgtgaa tatcttcatc
    32701 gaaattttcc agcagcagtt gctgggtctc gcgcattttt tggttgatgt cggcttctag
    32761 ctctttttgc aattgatcaa aagctgcttc tatttccgcc ggttgccgac aagtctcgta
    32821 tatattttgg atgcgttttt caaaatcaat accagattca atgctgccta gcacatcgtc
    32881 cgatgcacca aacacgccat cgaacagatg gaatttttca gtcagtaact ctaaaacacg
    32941 ctgatcggct ttgttggctt cgttgaggaa gttgatcacc acaacatcaa attgctgccc
    33001 atagcggtga caacggccaa tacgttgctc tactcgttgt gggttccatg gtaagtcata
    33061 gttaataagt aacgaacaga actgtaagtt aaccccttct gcagccgctt cggttgcgat
    33121 cataacttcg gcatgatggc ggaagtaatc aattagggca gtacggcggt caatttgcgg
    33181 tgaaccactc accttatcac taccttggtt ttcagctaac cactgctgat aaactttggt
    33241 tgcttctgga tggttattgc tgccactaaa ggtgactaat ttatcagcaa agccattcgc
    33301 ctcgaggaaa cgagctaggt attcttgcgt gcgcttcgat tcggtaaaaa taatgacttt
    33361 acgtggcgcc cccatttcct ccattttggc aaacccttga ttaagcgccg ttagcaaggc
    33421 ttgggtttta ctgtcggtgg taatggattt agcctgctcg atataatgct caagctcggc
    33481 tatttcagca ttgagtaact caaagtcaat atcactctca ggggtattgc tttgtgtttc
    33541 ttctacatac tcgctgtact cttctcctaa atcatcactc tcaacaagct cactgataat
    33601 atcgaggtcc gatttagcct gtactttcaa gtcaaccaat cggcgcttta tagtttcgag
    33661 tgtgttgagc actgcgtgcg aactagatgc taatagcttt ctcagtataa ggccggttaa
    33721 atgacgttgg ctcttgggta gtgcatagct ctcgtccttt tccaataagg cactaatact
    33781 ttcgtaaagt gccatctcag cattagaggg tgtaaacggt acggtgagtg tttgacgttg
    33841 ggtatagcgg acgtattcga gtacatcttt gcgcaaggta cgctgaataa acccggtaag
    33901 tcgtttcttt aaatgcccta gcgcactttc gttattgaca aagtgttgtt tgaagcttcg
    33961 ctcatcgccg aagatttgat cgtcgataat ggttgaaagt ccatacaatt ccatgagcga
    34021 gttttgtaag ggtgttgcag taagtaatag ctttttactg ccatctaatg cgcggcgtag
    34081 cgtttgaccc attttattgc tgctacgatg tgcattacgt agcttgtgcg cttcgtcgat
    34141 gacgacaata tcccatggct ctagcattag ctgcgtttcc attctcgcag catagtgata
    34201 ggacataata gaaacgcagt gattagctag cgggttagtt tgaccattgt taacactaat
    34261 gcgccacgtt tttgcatcaa gcacttgcgt tggtaaatta aacttatcgc ccaattcctg
    34321 cgcccactgg cgtcgcaatg acgccgggca gatgatcaat atgcggcgtt tacgctctgc
    34381 ccaatactgt gacagtacta agcctgcttc aatagttttc cctaagccca cttcatcagc
    34441 gagtacaaca cctttgctta acgggttgtt gagcgcaaaa agtgccgcat gaatttggtg
    34501 cggattgaga tcaacactgg catcaaaaag agattgagac aatttatcaa cgccattttc
    34561 agcgtggcga atggttaatt cctgcgcaaa gtattttgct tgataatcag ttattaccat
    34621 ccaacacccc ttttccttga gctgcttgcg tactgatcca ttcttcaaga tcggattgtt
    34681 taaagcgcca agcattgcca actttaaaag caggaatttt atgtaccgct gctagacggt
    34741 aaatagttct ctcatttacc ttcaaatact cagctacttc tttaattgtc agaacggtat
    34801 cagtcatttt cactcggcca ctttgaaaag tgcgtcaatt atctaatgcg agtgtcaaaa
    34861 tagtacaaat aagtacatga atcaatctta ttcggcactt gccggagctc cttggcttta
    34921 atcctcatca attttaggta cagttttcag gctcggggat aactacaccc tgaaccgtga
    34981 cagcgatgaa gactggtgtc ctcgaacgca tccgcgatat tcacgccagt atgcgccgtg
    35041 tttatttgca ggtgatttgc ccccctgctg actctcagcc aactgtatcg ccattaaggt
    35101 gaagatgttg ttttacacgc ttctgcacac ccttttttat acgactattc tattccttta
    35161 acagctggca aaaaaaactg acctataaac ctc
//
LOCUS       DAZKHC020000043        34524 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZKHC020000043 DAZKHC020000000
VERSION     DAZKHC020000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.043"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..2336
                     /gene="ynfE"
                     /locus_tag="ACHOSM_RS12030"
                     /old_locus_tag="pgaptmp_002406"
                     /pseudo
     CDS             1..2336
                     /gene="ynfE"
                     /locus_tag="ACHOSM_RS12030"
                     /old_locus_tag="pgaptmp_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
     gene            2397..4820
                     /gene="ynfF"
                     /locus_tag="ACHOSM_RS12035"
                     /old_locus_tag="pgaptmp_002407"
     CDS             2397..4820
                     /gene="ynfF"
                     /locus_tag="ACHOSM_RS12035"
                     /old_locus_tag="pgaptmp_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HGU4558911.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNYGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQAATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            4831..5448
                     /gene="ynfG"
                     /locus_tag="ACHOSM_RS12040"
                     /old_locus_tag="pgaptmp_002408"
     CDS             4831..5448
                     /gene="ynfG"
                     /locus_tag="ACHOSM_RS12040"
                     /old_locus_tag="pgaptmp_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HGU4558912.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            5450..6304
                     /gene="ynfH"
                     /locus_tag="ACHOSM_RS12045"
                     /old_locus_tag="pgaptmp_002409"
     CDS             5450..6304
                     /gene="ynfH"
                     /locus_tag="ACHOSM_RS12045"
                     /old_locus_tag="pgaptmp_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HGU4558913.1"
                     /translation="MGNGWHEWPLVIFTVLGQCFVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARITFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            6347..6961
                     /gene="dmsD"
                     /locus_tag="ACHOSM_RS12050"
                     /old_locus_tag="pgaptmp_002410"
     CDS             6347..6961
                     /gene="dmsD"
                     /locus_tag="ACHOSM_RS12050"
                     /old_locus_tag="pgaptmp_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HGU4558914.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRKTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            7120..8412
                     /gene="clcB"
                     /locus_tag="ACHOSM_RS12055"
                     /old_locus_tag="pgaptmp_002411"
                     /pseudo
     CDS             7120..8412
                     /gene="clcB"
                     /locus_tag="ACHOSM_RS12055"
                     /old_locus_tag="pgaptmp_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
     gene            complement(8365..9060)
                     /gene="bioD"
                     /locus_tag="ACHOSM_RS12060"
                     /old_locus_tag="pgaptmp_002412"
     CDS             complement(8365..9060)
                     /gene="bioD"
                     /locus_tag="ACHOSM_RS12060"
                     /old_locus_tag="pgaptmp_002412"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU4558915.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(9185..10405)
                     /gene="mlc"
                     /locus_tag="ACHOSM_RS12065"
                     /old_locus_tag="pgaptmp_002413"
                     /gene_synonym="dgsA"
     CDS             complement(9185..10405)
                     /gene="mlc"
                     /locus_tag="ACHOSM_RS12065"
                     /old_locus_tag="pgaptmp_002413"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HGU4558916.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(10540..11433)
                     /gene="ynfL"
                     /locus_tag="ACHOSM_RS12070"
                     /old_locus_tag="pgaptmp_002414"
     CDS             complement(10540..11433)
                     /gene="ynfL"
                     /locus_tag="ACHOSM_RS12070"
                     /old_locus_tag="pgaptmp_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4558917.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            11540..12793
                     /gene="ynfM"
                     /locus_tag="ACHOSM_RS12075"
                     /old_locus_tag="pgaptmp_002415"
     CDS             11540..12793
                     /gene="ynfM"
                     /locus_tag="ACHOSM_RS12075"
                     /old_locus_tag="pgaptmp_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4558918.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(12842..12961)
                     /locus_tag="ACHOSM_RS12080"
                     /old_locus_tag="pgaptmp_002416"
     CDS             complement(12842..12961)
                     /locus_tag="ACHOSM_RS12080"
                     /old_locus_tag="pgaptmp_002416"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HGU4558919.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            13217..13525
                     /gene="asr"
                     /locus_tag="ACHOSM_RS12085"
                     /old_locus_tag="pgaptmp_002417"
     CDS             13217..13525
                     /gene="asr"
                     /locus_tag="ACHOSM_RS12085"
                     /old_locus_tag="pgaptmp_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HGU4558920.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            13618..13701
                     /locus_tag="ACHOSM_RS12090"
                     /old_locus_tag="pgaptmp_002418"
     CDS             13618..13701
                     /locus_tag="ACHOSM_RS12090"
                     /old_locus_tag="pgaptmp_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4558921.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            13801..14622
                     /gene="ydgD"
                     /locus_tag="ACHOSM_RS12095"
                     /old_locus_tag="pgaptmp_002419"
     CDS             13801..14622
                     /gene="ydgD"
                     /locus_tag="ACHOSM_RS12095"
                     /old_locus_tag="pgaptmp_002419"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HGU4558922.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDDVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPIFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(14661..14990)
                     /gene="mdtI"
                     /locus_tag="ACHOSM_RS12100"
                     /old_locus_tag="pgaptmp_002420"
     CDS             complement(14661..14990)
                     /gene="mdtI"
                     /locus_tag="ACHOSM_RS12100"
                     /old_locus_tag="pgaptmp_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HGU4558923.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(14977..15342)
                     /gene="mdtJ"
                     /locus_tag="ACHOSM_RS12105"
                     /old_locus_tag="pgaptmp_002421"
     CDS             complement(14977..15342)
                     /gene="mdtJ"
                     /locus_tag="ACHOSM_RS12105"
                     /old_locus_tag="pgaptmp_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HGU4558924.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            15754..16788
                     /gene="tqsA"
                     /locus_tag="ACHOSM_RS12110"
                     /old_locus_tag="pgaptmp_002422"
     CDS             15754..16788
                     /gene="tqsA"
                     /locus_tag="ACHOSM_RS12110"
                     /old_locus_tag="pgaptmp_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HGU4558925.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(16813..18201)
                     /gene="pntB"
                     /locus_tag="ACHOSM_RS12115"
                     /old_locus_tag="pgaptmp_002423"
     CDS             complement(16813..18201)
                     /gene="pntB"
                     /locus_tag="ACHOSM_RS12115"
                     /old_locus_tag="pgaptmp_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HGU4558926.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(18212..19744)
                     /gene="pntA"
                     /locus_tag="ACHOSM_RS12120"
                     /old_locus_tag="pgaptmp_002424"
     CDS             complement(18212..19744)
                     /gene="pntA"
                     /locus_tag="ACHOSM_RS12120"
                     /old_locus_tag="pgaptmp_002424"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HGU4558927.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            20268..21212
                     /gene="ydgH"
                     /locus_tag="ACHOSM_RS12125"
                     /old_locus_tag="pgaptmp_002425"
     CDS             20268..21212
                     /gene="ydgH"
                     /locus_tag="ACHOSM_RS12125"
                     /old_locus_tag="pgaptmp_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HGU4558928.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            21398..22780
                     /gene="ydgI"
                     /locus_tag="ACHOSM_RS12130"
                     /old_locus_tag="pgaptmp_002426"
     CDS             21398..22780
                     /gene="ydgI"
                     /locus_tag="ACHOSM_RS12130"
                     /old_locus_tag="pgaptmp_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4558929.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            22817..23539
                     /gene="folM"
                     /locus_tag="ACHOSM_RS12135"
                     /old_locus_tag="pgaptmp_002427"
     CDS             22817..23539
                     /gene="folM"
                     /locus_tag="ACHOSM_RS12135"
                     /old_locus_tag="pgaptmp_002427"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HGU4558930.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(23536..23871)
                     /gene="ydgC"
                     /locus_tag="ACHOSM_RS12140"
                     /old_locus_tag="pgaptmp_002428"
     CDS             complement(23536..23871)
                     /gene="ydgC"
                     /locus_tag="ACHOSM_RS12140"
                     /old_locus_tag="pgaptmp_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HGU4558931.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            24000..24719
                     /gene="rstA"
                     /locus_tag="ACHOSM_RS12145"
                     /old_locus_tag="pgaptmp_002429"
     CDS             24000..24719
                     /gene="rstA"
                     /locus_tag="ACHOSM_RS12145"
                     /old_locus_tag="pgaptmp_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HGU4558932.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLSKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            24723..26024
                     /gene="rstB"
                     /locus_tag="ACHOSM_RS12150"
                     /old_locus_tag="pgaptmp_002430"
     CDS             24723..26024
                     /gene="rstB"
                     /locus_tag="ACHOSM_RS12150"
                     /old_locus_tag="pgaptmp_002430"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HGU4558933.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            26100..27029
                     /gene="tus"
                     /locus_tag="ACHOSM_RS12155"
                     /old_locus_tag="pgaptmp_002431"
     CDS             26100..27029
                     /gene="tus"
                     /locus_tag="ACHOSM_RS12155"
                     /old_locus_tag="pgaptmp_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HGU4558934.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(27026..28429)
                     /gene="fumC"
                     /locus_tag="ACHOSM_RS12160"
                     /old_locus_tag="pgaptmp_002432"
     CDS             complement(27026..28429)
                     /gene="fumC"
                     /locus_tag="ACHOSM_RS12160"
                     /old_locus_tag="pgaptmp_002432"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HGU4558935.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNCERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(28572..30218)
                     /gene="fumB"
                     /locus_tag="ACHOSM_RS12165"
                     /old_locus_tag="pgaptmp_002433"
     CDS             complement(28572..30218)
                     /gene="fumB"
                     /locus_tag="ACHOSM_RS12165"
                     /old_locus_tag="pgaptmp_002433"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU4558936.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPETSENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            30417..31592
                     /gene="manA"
                     /locus_tag="ACHOSM_RS12170"
                     /old_locus_tag="pgaptmp_002434"
     CDS             30417..31592
                     /gene="manA"
                     /locus_tag="ACHOSM_RS12170"
                     /old_locus_tag="pgaptmp_002434"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HGU4558937.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAARLSELFASLLNMQGEEKSRALAILKSALGSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFITANESPVTVKGHGRLARVYNKL"
     gene            31693..33201
                     /gene="ydgA"
                     /locus_tag="ACHOSM_RS12175"
                     /old_locus_tag="pgaptmp_002435"
     CDS             31693..33201
                     /gene="ydgA"
                     /locus_tag="ACHOSM_RS12175"
                     /old_locus_tag="pgaptmp_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGU4558938.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETQLEEMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(33246..34511)
                     /gene="uidC"
                     /locus_tag="ACHOSM_RS12180"
                     /old_locus_tag="pgaptmp_002436"
     CDS             complement(33246..34511)
                     /gene="uidC"
                     /locus_tag="ACHOSM_RS12180"
                     /old_locus_tag="pgaptmp_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HGU4558939.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
BASE COUNT         8477 a         8918 c         8504 g         8625 t
ORIGIN      
        1 tttctttgcc gtttaccctg cgcagtgcag cagcagcggt acaacaggcc cgcgaaaaag
       61 tggtctgggg tgcctgttcc gtcaactgtg gtagccgctg tgcacttcgt ctacatgtta
      121 aagataatga agtgacctgg gtggaaactg acaataccgg cagcgatgag tacggcaacc
      181 atcaggtacg cgcctgtttg cgcggtcgct ccatccgccg gcgtattaat catcccgatc
      241 gcttgaatta cccaatgaaa cgcgtgggta aacgcggtga aggcaaattc gaacggatta
      301 gctgggatga agccctggat actatcgcca gtagcctgaa aaaaaccgtc gaacaatatg
      361 gcaatgaggc tgtatatatt cagtactctt cggggattgt tggcggcaat atgacccgct
      421 cttcgccatc agcctcggcg gtcaaacgcc tgatgaactg ctacggcggt tcactcaatc
      481 agtatggctc ctacagcacc gcgcaaattt cctgtgccat gccctacacc tacggcagta
      541 atgatggcaa cagcaccacg gatattgaaa acagcaagct ggtcgtgatg tttggtaaca
      601 acccggcaga aacccgcatg agcggcggtg gcatcactta tcttcttgaa aaagcgcgcg
      661 agaaatcgaa cgccaaaatg attgttatcg atccgcgata taccgatacg gctgctggtc
      721 gtgaagatga atggcttcct attcgcccgg gcaccgatgc cgcgctggtt gcgggtattg
      781 cctgggtatt gattaacgaa aatctcgttg atcaaccttt tctcgataaa tactgcgtcg
      841 gctatgacga aaaaacctta cccgcagatg cacccaaaaa tggtcactat aaagcctata
      901 ttcttggtga aggtgatgat aacacagcga aaacgccgca gtgggcttcg caaattaccg
      961 gtatcccggt agaccgtatc atcaaactgg cgcgtgaaat tggcacagca aaacccgctt
     1021 atatctgcca gggctggggg ccgcaacgcc aggcaaacgg cgaactgact gcacgcgcta
     1081 ttgctatgtt acctattttg acgggcaatg tcggcatcag cggcggaaat agtggcgcgc
     1141 gtgaatcgac ctataccatt accatagaac gcctgccggt gctggataat ccggtcaaaa
     1201 cgtcaatctc ctgcttcagc tggacagatg ctatcgatca tggtccgcaa atgacggcaa
     1261 tccgcggcgg cgtccgaggc aaagataaac tggatgtgcc cattaagttc atctggaact
     1321 acgcgggaaa taccctcgtt aatcagcatt ctgacatcaa caaaacgcat gaaattctgc
     1381 aggacgaatc gaaatgcgaa atgattgtgg tcatcgaaaa ctttatgacc tcatcggcaa
     1441 aatatgccga cattctgctg ccagacctga tgaccgttga gcaggaagat attattccta
     1501 acgactacgc cggtaacatg ggatatctca ttttcctcca gcctgtcacc agcgaaaaat
     1561 tcgaacgcaa accgatttac tggatcctga gtgaagtcgc gaaacgtctt ggacctgacg
     1621 tctatcaaaa gttcacagaa ggtcgcacgc aggaacaatg gttacaacat ctgtacgcca
     1681 aaatgcttgc caaagatccg gcgttaccgt cttacgacga actgaaaaaa atgggtatct
     1741 ataagcgtaa agatcccaat ggccattttg tcgcctacaa agcatttcgt gacgaccccg
     1801 aggcaaatcc acttaaaacg ccttccggta agattgaaat ttattccagc aggctggcgg
     1861 aaattgcccg tacctaggaa ctggaaaaag atgaagtgat aagcccattg ccagtttatg
     1921 cctcaacctt tgaaggctgg aactcccctg aacgtagaac cttcccactg caactgtttg
     1981 gtttccatta caaatcccgt actcactcga cctacggcaa tattgatctc ctgaaggctg
     2041 cctgccgtca ggaggtgtgg atcaacccta tagatgcgca gaaacgtggg attgccaacg
     2101 gcgatatggt gcgggtgttt aaccatcgtg gcgaagttcg gctaccagcc aaagtaacac
     2161 cacgtattct ccctggagtt agcgctatgg gccagggagc ctggcacgag gccaatatgt
     2221 ctggcgataa aatcgaccat ggcggctgtg tgaatacgct aaccactctg cgcccttcac
     2281 cactggcgaa gggaaacccg cagcacacta atctggtcga gatcgaaaaa atataaccca
     2341 cgacaaccat aaattctggc atgacatttt gtttgaaaag caataagtga gtaatgatga
     2401 aaatccatac cacagaggcg ctcatgaagg ctgaaatcag ccgtagaagt ctgatgaaaa
     2461 cctccgcact tggcagtctt gcgctggcaa gcagtgcttt cactctgcca ttttcccaaa
     2521 tggtccgggc ggcagaggct ccggtagaag agaaagcggt ctggagttcc tgcaccgtta
     2581 actacgggag ccgctgtctg ttacgtttgc atgtgaaaga tgacaccgtg tactgggtgg
     2641 agtctgatac gacaggtgac gacgtctacg gtaatcatca ggttcgagcg tgtttacgcg
     2701 ggcgctctat ccgccgacgg atgaatcatc ctgataggtt gaaatatccc atgaagcgcg
     2761 tcggcaagcg cggtgaaggt aaatttgaac ggataagttg ggacgaagcc ctggatacca
     2821 tcagtgataa tcttcggcgg atcctgaaag attacggcaa tgaggctgta catgtcctgt
     2881 acggaacagg cgtagatggc ggaaacatca ccaactcaaa cgtcccgtac cgtctgatga
     2941 actcttgcgg tggttttctc agtcgctatg gcagctacag taccgcacag atcagtgccg
     3001 caatgagtta tatgttcggt gccaatgatg gcaacagccc ggatgatatc gccaatacga
     3061 aactggtcgt tatgttcgga aataacccgg cagaaacgcg gatgagcggc ggtggtgtca
     3121 cttactacgt cgagcaagcc cgcgaacgtt caaacgcacg catgatcgtc atcgatccac
     3181 gttataacga cactgctgcc gggcgtgaag atgaatggct gcccattcgc cctggcaccg
     3241 atggcgcact ggcctgtgcg attgcctggg tactgattac tgaaaacatg gtcgatcagc
     3301 catttctcga caaatattgt gttggttacg atgaaaaaac gctgcccgcc aacgcaccac
     3361 gtaacgcgca ttataaagcc tatattctgg gcgaagggcc tgacggcata gctaaaacgc
     3421 cggaatgggc agcaaaaatc accagcatcc cggcagaaaa aattatccag ttggcgcgag
     3481 agatcggttc agcaaaacct gcttatattt gtcagggttg ggggccacaa cgacattcca
     3541 acggcgagca aacgtcccgc gctattgcca tgctttccgt tctcaccggt aacgtcggca
     3601 taaacggcgg caactcaggt gtacgcgaag gtagctggga tctgggggta gaatggttcc
     3661 cgatgcttga gaatcctgtt aaaacgcaga tttccgtctt tacatggaca gatgccatcg
     3721 accatggtac ggaaatgacc gcgacccgtg atggtgttcg tggaaaagaa aaactggatg
     3781 tccccatcaa gtttttatgg tgctacgcca gtaacacatt gatcaatcaa catggcgaca
     3841 tcaatcacac ccatgaggtg cttcaggatg acagcaagtg cgagatgatt gttggcattg
     3901 accacttcat gacggcctcg gctaagtatt gcgatatcct gttgcccgac ctgatgccaa
     3961 cagagcaaga agaccttatc tctcatgaat ctgcagggaa tatgggctat gtgatcctcg
     4021 cccaagccgc aacctcagca aaatttgaac gcaaacccat ctactggatg ctgagtgaag
     4081 tcgccaaacg cttaggacca gacgtttatc aaacctttac tgaaggtcgc agtcagcatg
     4141 aatggatcaa atatctccat gcgaaaacga aggaacgtaa ccctgagatg cccgactacg
     4201 aggagatgaa aacgaccggg atctttaaga aaaaatgccc ggaagaacac tacgtcgctt
     4261 tccgcgcatt ccgtgaagat ccacaggcaa acccgttgaa aacaccttcg gggaaaatcg
     4321 aaatttattc tgaacgactg gcgaagattg cagatacctg ggaattgaaa aaagatgaaa
     4381 ttattcatcc ccttcctgcg tataccccag gttttgatgg ctgggacgat cccctgcgga
     4441 aaacctatcc actgcagtta acgggcttcc attacaaagc gcgtacccac tccagctacg
     4501 gcaatattga tgtgttacag caggcctgcc cacaagaggt gtggatcaac cccattgatg
     4561 ctcaggcacg cggtatccgt catggcgata ccgtgcgggt atttaacaat aatggagaaa
     4621 tgctgattgc cgcaaaagtg actccgcgta ttctgcctgg cgttaccgcc atcgggcaag
     4681 gtgcgtggct taaagcggat atgtttggtg accgggtcga tcacggcggc agtatcaata
     4741 ttctgacctc tcaccgccct tcaccgctgg caaagggaaa cccgtcgcac agcaatcttg
     4801 tccagatcga aaaggtttaa ggagtagccc atgaccacac aatatggatt ttttatcgat
     4861 tccagccgct gtactggctg taaaacctgc gaactggcgt gcaaagattt taaagatctt
     4921 ggcccggaag tcagtttccg ccgtatttat gaatacgctg gcggcgactg gcaggaagat
     4981 aatggcgtct ggcaccagaa cgtgttcgct tattatctct ccatctcctg taaccattgc
     5041 gatgaccccg catgtacaaa agtctgcccc agcggcgcaa tgcataagcg tgaagatggc
     5101 tttgtggtgg ttgatgaaga tgtttgtatc ggctgccgct actgccacat ggcttgtccg
     5161 tacggcgcac cacagtacaa tgctgaaaaa gggcacatga cgaagtgcga tggttgttat
     5221 tcgcgcgtcg ccgaggggaa acaacccata tgcgtcgaat cctgcccgct gcgtgcactt
     5281 gagttcggtc caattgaaga gctgcgccag aagcacggca ctctggctgc cgtcgcgcca
     5341 ctgccgcgcg cgcatttcac aaaacccaat atcgttatca aacctaacgc caacagccgc
     5401 ccgaccggcg ataccactgg ttatctggct aatccggagg aagtgtaaaa tgggaaatgg
     5461 atggcatgaa tggccactgg ttatctttac cgtattgggt caatgctttg taggcgcgct
     5521 gattgttagc ggaattggct ggttcgccgc caaaaatgac gcagaccgtc agcgtattgt
     5581 ccgtggcatg tttttcctct ggctgttgat gggcatcggg tttattgcct ctgtcatgca
     5641 cctgggatcg ccactgcgag cgtttaactc gctgaatcga atcggagctt ctggtctgag
     5701 taacgagatt gctgccggtt ccatattttt tgccgttggc ggactatggt ggctggtggc
     5761 ggtcatcggt aaaatgccgc aagcgttggg gaaactctgg ttacttgtca gcatggcgct
     5821 cggcgtcatt ttcgtctgga tgatgacctg cgtgtatcaa atcgacaccg tgccaacctg
     5881 gcataacggt tacactacgc tggctttctt cctgacggta ttgttgagcg gtccaatact
     5941 tgctgccgca attctgcggg cagctcgcat tacatttaat accacaccat ttgccatcat
     6001 tagcgttctg gcgctgatcg cttgtgccgg ggtgattgtg ctgcaaggtt tgtctctggc
     6061 gtctattcac tcatccgtgc aacaagccag cgcactggta ccagattacg cctccttaca
     6121 ggtctggcgt gtggtattgt tatgcgcagg tcttggatgc tggctttgcc cgctcattcg
     6181 tcgccgtgaa cctcacgttg ccggacttat tctggggcta atcctcatac ttggcggtga
     6241 gatgatcggg cgtgtgctct tttatggcct gcacatgaca gtaggaatgg ctattgcagg
     6301 ttaacaaatt ggtgcggggc aacccgcaca tttcaggatg ttaggaatga cccatttttc
     6361 acagcaagat aatttttctg tcgcggcgcg cgtgttgggc gcgctgtttt attacgctcc
     6421 agagagtgcg gaagccgcac ctctggttgc ggtactcacc agtgacggct gggaaactca
     6481 gtggcctcta ccagaagcgt cattagcgcc gctggtgacg gcttttcaga cacagtgtga
     6541 agagactcac gcccaggcct ggcagcgttt gtttgtcggc ccgtgggcac tgccgtctcc
     6601 gccgtggggg tcggtctggc tggatcgcga atctgtgctg tttggcgatt caacattggc
     6661 acttcgtcag tggatgcgcg agaaaggcat tcagtttgaa atgaagcaaa acgaaccgga
     6721 agatcatttt ggttcactgc tgttgatggc tgcgtggttg gcggagaatg gtcgcaagac
     6781 agaatgcgaa gaattgctgg catggcacct ttttccgtgg tcaacacgtt ttcttgatgt
     6841 ttttatcgaa aaagcagaac accctttcta ccgtgcactg ggtgaactgg ctcgcttaac
     6901 gctggcgcaa tggcaatcac aactgttaat tcctgtcgcg gttaaaccgc tgtttcgata
     6961 ggatcaccgt aatattgccg gatggtgacg attacgcgca atccggcaat aataggttac
     7021 agcgtcacgt ttttttatct cttaaagcac gcactgcttt tgcggctggc ctcttttgcc
     7081 gcaaaatagt cgcccgtgtt tcattgccca tttctgctca tgcatcatct acacatctat
     7141 ccggatctgc gcactatgtt ccgccgtctg cttatcgcaa cagtcgtcgg tattctcgcg
     7201 gcctttgccg ttgccgggtt tcgtcatgcg atgctgctac tggagtggtt gttcctcaat
     7261 aatgactccg gcagtctggt caatgcagcg acaaaccttt ccccctagcg acggttgcta
     7321 actccggcgc tcggcggact ggcggcgggt ttgttgctga tgggctggca gaaatttacc
     7381 caacaacgcc ctcatgcgcc gaccgattac atggaagcgt tgcaaaccga tggacagttc
     7441 gattacgcag caagcctggt taaatcgctt gcctcgctgc tggtagtaac cagcggcagt
     7501 gcaattggtc gcgaaggtgc gatgattctt ttagctgccc ttgccgcctc ctgttttgcc
     7561 caacgtttta cgccacgcca ggagtggaaa ttatggatcg cctgtggggc cgcggcggga
     7621 atggctgcgg cctatcgtgc cccgcttgct ggcagtttat ttatagccga agtgctgttt
     7681 ggcactatga tgttggcctc tctcggcccg gtgattattt ccgccgtcgt ggcattgctg
     7741 gttagcaatc tgattaatca tagcgacgcg ttactctaca acgtacaact ctcagtgacg
     7801 gttcaggctc gtgactatgc gctgattatc agtacaggtg tgctggcagg tctgtgcgga
     7861 ccactgttgt taacgttaat gaacgcctgt catcgtggat ttgtgagtct caaacttgcg
     7921 ccgccctggc aactggcact aggcgggttg atcgtgggtc tgctttccct gttcacacct
     7981 gcagtgtggg gcaacggcta tagcaccgta caatcctttt taaccgcccc accactgtta
     8041 atgatcattg ccgggatctt cctttgtaaa ctgtgtgccg tgctggcgag tagcggttcc
     8101 ggcgcacccg gtggggtctt tacaccgacg ctatttatcg gtcttgccat tggcatgttg
     8161 tatggtcgta gcctgggatt atggttccct gatggcgaag aaattacact tttactcgga
     8221 ttgaccggga tggcgacact gttggcggcg accacgcacg cgccgattat gtcgacgttg
     8281 atgatatgtg aaatgaccgg ggagtatcag ctactccccg gtttattgat tgcctgcgta
     8341 attgcgtcgg taatttcgcg gacgttacac cgtgactcta tctaccgcca gcacactgcg
     8401 cagcatagct aagcggatgt attgccccag ttcacgctgt tcggcgcgcg gcagataagg
     8461 cagttcacca atgagcggtg ccggaagttt tttacccagc acatcaatga tttccgcata
     8521 atgcgccagt cctgggttga ttcggttagc cacccagcca atgagcggca gcccgtcgtt
     8581 ggcgatcgcc tgagctgtta gcagtgcatg gttaatgcaa ccttcctgaa taccgacaac
     8641 catcaacacc ggcagttgtt cctgcactac ccattcagag agtggacgca aatcattcat
     8701 cagactgcgc cagccgccag tcccttctac cacgacatga tcgacttttt cggtcaggtt
     8761 tgccaggccg tttgaaatga gggtgtaatt gattgggcaa ctgtgcgcca cgctactttc
     8821 ttcttcgctt aacgcgatag gattaactgc ttcataaggc agttcgatgg ttgaaacact
     8881 ctgcaacacc agggcatctt tattacgcag cccttcgggt gtctctttgc tccccttcgc
     8941 tacgggttta tatcccgcaa ccgtttttcc ctgggaggct aacgcttgta gcaatgcgcg
     9001 ggaaaccacc gttttcccta cagaagtgtc tgtaccggta ataaagaaac gcttcagcat
     9061 cactaactcc accgttatgc ttcacaaata taaaccaaga aaataattaa ccttgaaagt
     9121 ctaagttatg ctttcctggc ccaaattgag atagcgcaaa ttttggtaga acagttaaaa
     9181 aatgttaacc ctgcaacaga cgaatcaaca aagaaccgtt atacatcgcg tcttttacca
     9241 gcgcagcccc tgccatcgta ccctggttag aaaactgagt actctcaacg ctgatgtgct
     9301 gactatacgc aggaagggcc tgctgacgga tgctgtctga gatgaccggg aagaggatat
     9361 ctgccgcttt acttaacggt gagccaatca gtattttttg tgggttaaat aaattcacca
     9421 tgatggcaag aatgcgccct acatgcgcgc ccaccccggt aatgatgtct tttgccagca
     9481 gatcgccgcg caatgccgcc tgacacaatg agtccacggt taacggttgt ccatgtaaca
     9541 tcgagctcat ggattgatta agacgcagct gtgccagctc aagaatactg tccacactgg
     9601 cgatggtttc gaggcagccg tgattcccgc aataacagcg tttcccatac gggtcgacct
     9661 gtgtgtggcc tatttccacg agactactgc tgcctgcgtg tagcagatga ccatcggtaa
     9721 tgacgcccgc ccccacgttg tgatcgataa ccacctgaat cacatcgcgc gccccgcgtg
     9781 aggcaccaaa caaggcctct gccatcgtcc atgcgctaat atcatgctga atataaaccg
     9841 gtacgccggt atgctgctcc agcgcctcgc cgagcggcat ctcttttaca tcctcgtaga
     9901 acggcatgcg atgtacaata ccattttccg tatcaataat tcccggcagg gttatggcaa
     9961 tcgaagttag acgctcaagt tttttctggt ggcggataaa aaactgatcg atatgggaaa
    10021 tgatacgatc cagcaatggc gagtcatctt ttaacgccag ttcctgcgac tcttccacca
    10081 ccagtttgct gctcagatcg cgcagagcaa ggaaaatctc cccgcgacta atgcgcagag
    10141 aaagatagtg ccaggcttca gtttcaacca ccagccccac cgccggacgg ccacggttcc
    10201 ccgcttcttt gatttccagc tcttgcacca ggtgtgcttc gagcatctca cggacaattt
    10261 tagtgatact ggcaggagcc agctgcgcca gacgggaaag atcgatacgc gagactggac
    10321 caagctgatc aatcaggcga taaaccgcgc ccgcgttggt ctgctttatt tgatcaatgt
    10381 gcccaggctg gttttcagca accaccgcat actccctata ttttcgcgct ccgaaataat
    10441 ctgtaggcta tggtgaagca cttcaatacg tgtcgtcaaa tttttactta ggcatgtgat
    10501 taacagcaca tttttcgggc tttttcgctg aaatttccct cacctgagag cattcagcag
    10561 atgaatacga aagttacgcg cagccggact ttgttcatga tgtttcggcc agaccaacca
    10621 catttcagaa accgcatcct cttcagcaat cggcacccag cgcatttcgt tgagctgaac
    10681 acgtttaaat gaagcaggca aaattgaaac acccagaccg gcggaaacca gaccgatgat
    10741 ggtcattgcc tcgcccacct cctgagtgat gacgggcgtc aaatggtaac gtcgcatcag
    10801 cccgagaata tcgtcataca gccctgtccc gacgtgcgga tcaaaaaaga cgaagggttc
    10861 tttcgccagt tcagccagcg ttacattcgg gttatttgcc aggggatgat cgtgcgggat
    10921 catcgccata agcggttcat ggacgatgac tgcgtgttca agcgtctccg gtaacgctgt
    10981 gttacgcagc aatcccatat ccagcgttcc ttcaatgagc ggagcgattt gctcacgagt
    11041 gttcatttcg cgggtttgta aatggacatc gggataatca cggcgaaaca gcgataacgt
    11101 atcggacacc gcccgaataa aaggagcgga cgaagtaaaa ccaatgcgca actcccccgc
    11161 ttccccctga tgcagtcttt cagcgcgagc ggcagcgtca tctaccatag acaggatttg
    11221 ccgactatct gcaagaaact gttttcctgc tgccgtcagc aatacactgc gattagttcg
    11281 tgccagcagt cgggcaccaa tttgttgctc cagtgcctga atctgctgac ttagcggtgg
    11341 ttgcgaaata ttcaggcggg cagcggcgcg cccgaaatgc agctcttccg caacagcaac
    11401 aaagtaacgc agatgacgaa gttcaatatt catatttaaa acatcttatt tgagattatt
    11461 aatatattag acagaacaat tcgattttcc taacctatgt ataagcctga tccacaggca
    11521 tatttagcaa ggatttcaag tgagccgtac tacaactgtt gatggcgctc cggcaagcga
    11581 cactgacaag caaagcattt ctcagccaaa tcaatttatt aaacgcggta cgccgcaatt
    11641 tatgcgcgtc accctggcgc tgttctctgc cggactggca acatttgcac ttctctattg
    11701 tgtgcagcct atccttccgg tgctttcgca ggagtttggc ttaacgcccg cgaacagtag
    11761 tatttcactg tccatttcca cggcgatgtt ggctattggt ttgctgttta ctggccccct
    11821 atccgatgcc attggtcgca aaccagtgat ggtcacggcg ctactgttgg cctccatttg
    11881 tacgttactt tcgacaatga tgaccagctg gcacggcatt ttgattatgc gcgccttgat
    11941 tgggctttcg ttaagtggcg tggcagctgt tggcatgact tatcttagcg aggaaatcca
    12001 ccccagtttc gtggcctttt cgatggggtt gtatatcagc ggcaactcaa ttggcggcat
    12061 gagcggacgc ttaattagtg gtgtcttcac ggactttttc aactggcgaa ttgctctggc
    12121 ggcaatcggt tgtttcgcgc tggcctcggc gttgatgttc tggaaaatcc ttccggaatc
    12181 acgccatttt cgcccgactt cgctgcgccc taagacgtta tttatcaact ttcgtctgca
    12241 ctggcgtgac cggggattac cgttattgtt cgcagaaggc tttttgctga tggggtcgtt
    12301 cgtcacgctg tttaattaca tcggctatcg gttgatgctt tccccctggc atgtcagtca
    12361 ggccgtggtt ggcttattat cgctggctta tttgaccggt acatggagct cacccaaagc
    12421 cggaaccatg accacccgct acgggcgcgg tccggtgatg ttgttttcga cgggggttat
    12481 gctgtttggt ttactgatga ccttattcag ctcgctgtgg ctgatctttg ccggaatgtt
    12541 actcttctca gcaggattct tcgcagccca ctcagttgcc agcagctgga ttggcccccg
    12601 cgcaaaacgc gctaaagggc aggcctcctc gctgtatctg ttcagttact atctggggtc
    12661 gagtattgcc gggacgctgg gtggtgtttt ctggcataac tatggctgga acggcgtcgg
    12721 cgcatttatt gctctgatgc tggtcattgc tctgctggtc gggacgcgtt tgcatcgtcg
    12781 tctgcacgcc tgaaaaataa gcccggactg cggtaactac ccgtccggac ttattgcttg
    12841 ctcaaaccaa cgttaatagc catcctaaaa tagacgaagc gccagccaat tcccgcagcg
    12901 cgtctagcgt catcaggatt ataagtaccc aaataaacgg attcattttg ctgtgtgtca
    12961 tttattactg atgcgcagtt attctaccgc tttgtaagta gtaaaatagt taacccgatc
    13021 aagactacta ttattggtag ctaaatttcc cttaagtcac aatacgttat tatcaatgct
    13081 gtaatttatt cagcgtttgt acatatcgtt acacgctgaa accaaccact cacggaagtc
    13141 tgccattccc agggatatag ttatttcaac ggccccgcag tggggttaaa tgaaaaaaca
    13201 aattgagggt atgacaatga aaaaagtatt agctctggtt gttgccgctg ctatgggtct
    13261 gtcttctgcc gcctttgctg cagagactgc gaccacacct gctccgactg cgacgaccac
    13321 caaagcagcg ccggcgaaaa cgacacatca taaaaaacag cataaagcag cacctgccca
    13381 gaaagcgcag gcggctaaaa agcatcataa aaatacgaaa gctgaacaga aagcccctga
    13441 acaaaaagcg caggcagcga agaaacacgc cgggaaacac agccatcagc aaccggcaaa
    13501 acctgctgca caacccgcag cgtaagttta caacagtaat gctggcgcgc cccctcgcgc
    13561 ctgaaaatta cggtgctaag cgggtaacgt ttagcaccgc ctttagcgga gggtagtatg
    13621 gtgggccgtt atcgctttga gttcattctt atcatcctta ttttatgcgc actgattgcc
    13681 gcccgttttt atctttcctg actgtagtta tctgatttta ctcccacttt catcccgtcc
    13741 cgtctatagt atttatgagg gtttgctttt aataatcata attacccacc agagtgtgat
    13801 atgcgtacaa ccattgctgt agtgttgggt gcaattagtt tgacgtctgc ttttgtgttt
    13861 gcagataaac cagacgttgc caaatcggca aacgatgatg tcagcaccct gttttttggt
    13921 catgatgatc gtgtgccagt gaatgacacg acccaatcac cgtgggatgc ggttgggcaa
    13981 ctggaaacgg ccagcggcaa tttatgtacg gcgacgctga ttgcacccaa tctggcatta
    14041 acggcaggac actgtttatt gacgccgcct aagggtaaag cggataaggc ggtagcgctg
    14101 cgttttgtgt caaataaagg tctttggcgc tatgacatcc acgacataga aggccgcgtt
    14161 gatccgacac tgggaaagcg gttaaaagca gatggggatg gttggattgt tcctcccgca
    14221 gctgcaccat gggacttcgg cttgattgtg ctgcgtaatc ccccttctgg cattacaccg
    14281 ttgccgatat ttgagggaga taaagccgcg cttactgccg cattaaaatc ggctggtcgt
    14341 aaagtgactc aggcgggata tcctgaagat catctcgata cgttatacag tcatcaaaac
    14401 tgtgaagtga ctggctgggc gcaaacgtcg gtgatgtcac atcagtgcga taccttgccg
    14461 ggtgacagcg gttcgcctct gatgttgcat accgatgacg gctggcaatt aattggggtg
    14521 caaagttcgg ctcctgccgc gaaagatcgc tggcgcgccg ataaccgggc catttctgtt
    14581 accggttttc gcgacaagct ggatcaactg tcgcaaaaat aatgttcaaa cgctgcccga
    14641 cagtgcgggc agcgtgttca tcaggcaagt ttcaccatga tcattccagc caacagcaag
    14701 accaggccaa tccagccttt acgatttaac cgttgaccaa acaagatcca acctgcggct
    14761 aacgtggcgg caataccaaa cccgccccac aatgcataag cgacagacaa gtcgatccct
    14821 tttacggcct gagaaagcgc actaaaggca gccagcaccg ccgccaggga gagcaagcca
    14881 aatattttgc gacgaaagcc gtcagaaaat ttcaaaaaga cgttagcaac gatttccagc
    14941 acgattgcca atgccagcca ggcggcgtga acccattcaa actgcgccat ggttcacctc
    15001 cagttcaggt ttacgcgctt tacgggtacc tgatttgatc aacacaatcc cggcgaccag
    15061 ggtggttaac ccggcaattt tcatcagcga taaactttcg tcgaataaca aaacgctaaa
    15121 caaggtaata aataaaatac cgataccttc ccacagcgca taagctacgc ctaaggcgat
    15181 ctttttaacg gcgaaagaga gaaatatata cgacagagaa atcatcacca gcattaaaat
    15241 aaagccgcca tttccctcac tgacgctcgc ccatttcatt gacagcgtac cggtaatttc
    15301 tgtagcaata gccaggccta ataaaatcca ataaatatac attgtccttc tcctgcaaga
    15361 gaattatttt aattttcgct taattcagcg aaaccaaagt aaagtggtca agccccgcgc
    15421 atagggcaag cttaggcaga agaaaggact aaagcgcgtt gcgccagtgc tgctcaccta
    15481 cgagcaagat agtggatgag gtacgaagat gggtaaatgt tgaaaataac gtcctgaaca
    15541 aattgtccat aatattacaa ttatccgcag tgttgcttct cgtcatcgcg gatgataatt
    15601 gtcctcggta gttgaacacg cctgatttgt atcatagctt aagaattaac tcaaaatatt
    15661 ttcacttctt tacctgagcg gtttgatttt cgttatgatg acggagcgaa aaagacatta
    15721 ttattagcaa aggaagaaaa aacggggaca agcatggcaa agccgatcat cacgctcaat
    15781 ggcctaaaaa tcgtcattat gttgggaatg ctggtcatta ttctctgcgg tatccgtttt
    15841 gccgccgaga tcatcgtgcc gtttattctc gcattattta ttgctgttat tcttaacccg
    15901 ctggtgcaac acatggtccg ctggcgagtg ccgcgtgtac tggcggtgtc gattttgatg
    15961 accatcatcg tgatggcgat ggtgttgcta ttagcttatc tgggttccgc gctcaacgag
    16021 ttgacgcgga cgttaccgca atatcgcaac tctattatga cgccgctgca agctcttgag
    16081 ccgttgttgc aacgcgtagg gattgacgtc tcagttgacc agctggcgca ttacattgat
    16141 ccgaacgcgg cgatgacgtt gctcaccaac ttattgacgc agttatctaa tgccatgtca
    16201 tcaatatttt tattgctgct gacggtgctg tttatgctgc tcgaagtgcc acaattgccc
    16261 ggaaaatttc agcaaatgat ggcgcgtccg gttgaaggga tggcggcgat tcaacgtgca
    16321 attgacagcg tttctcatta tctggtgctg aaaacagcca tcagcatcat caccggcctg
    16381 gtcgcctggg cgatgctcgc cgcactcgat gttcgcttcg cttttgtctg gggattgctg
    16441 gcctttgcgc ttaattacat ccctaatatt ggttcagttc tcgcggcaat cccccctatc
    16501 gctcaggtac tggtgtttaa tggcttctac gaagcgttgc tggtgctggc gggatatctg
    16561 ctgattaatc tggtcttcgg caatattctg gagccgcgca tcatgggacg tgggctgggg
    16621 ctttccacat tggtggtatt tttgtcgttg attttttggg gatggttgtt aggaccggtg
    16681 ggtatgctgc tttccgtgcc gttaacaatt attgtcaaaa ttgcgcttga acaaacagcg
    16741 ggaggtcaaa gcatcgccgt tctgttaagc gatctcaata aagagtgacg gcctccgcag
    16801 aggccgtcag ggttacagag ctttcaggat tgcatccacg ctggctttgg cgtcaccaaa
    16861 cagcatgtgg gtgttttcct tgaagaacag cgggttttgc acaccagcat agccagtgtt
    16921 catcgaacgt ttaaagacaa tcacgttctg cgctttccac acttccagca caggcatacc
    16981 agcaatagga ctcttcggat catcctgcgc cgccgggtta accgtatcgt tagcaccaat
    17041 caccagtacg gtatcggtat cagcaaagtc atcgttgatc tcgtccattt ccagcacgat
    17101 gtcatacggt acttttgctt cagccagcaa tacgttcata tgtccaggca aacgccccgc
    17161 aaccgggtgg ataccgaaac gcacgttgat accacgagcg cgcagtttct cggtaatttc
    17221 agcgacagga tattgcgcct gcgcgactgc catgccgtac cccggagtaa tgatcactga
    17281 atgggagttt ttcagcagtt ccgctgtctc ttctgcggtg atttcgcggt gctcgcccac
    17341 ttcctgatca tcgccagtag aagagccgtc ggtgccgaaa ccacccgcaa taacgctgat
    17401 aaaggaacgg ttcatcgcct tacacataat gtaagaaagg atagcccccg aagaaccgac
    17461 cagcgcaccg gtcacaatca gcaggtcgtt gctgagcata aagcccgcag ccgcagccgc
    17521 ccagccggag tacgagttca gcatcgacac caccactggc atatctgcac caccgatgga
    17581 ggcgactaaa tgccagccga ataccagcgc aatggcggtc attattagca atgccagcac
    17641 ttgcaggccg acgctgtccg tgcgaacaaa tacaatcagc agcaggaagg aaacgaccag
    17701 agccgccagg ttcattttgt gacggtttgg cagcatcaac ggtttagacg aaatcttgcc
    17761 acacagtttg ccgaacgcca ccaccgaacc cgtgaacgtt accgccccga tgaagatacc
    17821 gaggaacact tccgtcaggt gaatattgac cagaatcggt gccattcccg cgtcatgatg
    17881 cagatagctg ttaaagccaa ccagcactgc cgccagaccc acgaagctgt gcaggatcgc
    17941 caccagttct ggcatttcgg tcatttcaac tttcttcgcc agacggatac caattgcccc
    18001 accaatgacc atcgcaagca agatccagcc aacattaccc gtatccggtc caaaaatggt
    18061 tgcgattaac gcaatcgcca tcccggcgat accgaagttg ttaccctggc gagacgtttc
    18121 atgtttcgaa agaccggcca gactgaagat aaacaggatc gcggcaacaa tgtatgcagc
    18181 tgtaactaat cctccagaca tatgttaccc cttaattttt gcggaacatt ttcagcatgc
    18241 gctgagtcac ggtgaagcca ccgaaaatat taatgctggc tataagcacc gcgataaaac
    18301 taaggaagct aacccagccg ccctggccaa tctgcaacag tgctccgaca acaataatcc
    18361 ctgaaatcgc gttggtgacc gacatcaacg gtgtatgcag cgcgtgcgat acattccaca
    18421 ccacgtaata accgacaacg caggccagcg cgaaaacggt gaagtgccca agaaactctt
    18481 tcggcgcaac gcttgccagc caaccgaaaa ggatgattgc cagcgccatc aacgcgtatt
    18541 tacgccacgg tgagcaggca catttttcct cagttttcac ttccggtgcc gctttttgtg
    18601 ccgcctgcgg ctgagctgat acctgaatcg gcggtgccgg ccaggtaatt tcgcccgcac
    18661 ggatcacggt cacgccgcga atcaccacat catcaaaatc aacagtgata ttgccgtctt
    18721 tctctttgca caacagtttc agcagattaa cgaggttagt accgtaaagc tgcgaggatt
    18781 gcgtcggcag acggcccgga agatcggtat aaccaatcac tttgacacca ttttccgtag
    18841 tgaagatttc acccggcacg gtgtattcac agttgccacc gttttgggct gccaggtcga
    18901 caatcacact gcccgccttc atggagtcaa ccatttcacg ggtaattagc ttcggcgctg
    18961 gtttgcctgg aataagcgcg gtggtgacaa tgatatcgac ctcttttgcc tgggcggcaa
    19021 agagttccat ttccgcttta atgaacgcgt ccgacatcac tttggcatag ccatcgccgc
    19081 tgcccgcttc ctctttaaaa tccagctcga ggaattccgc gcccatactt tgaacttgtt
    19141 ctttcacttc cggacgggtg tcgaatgcac gcacaatcgc gccgagactg tttgctgcgc
    19201 caatggcggc cagacctgca acacccgcac caatcaccat cacttttgcc ggtggcactt
    19261 tcccggccgc agtaatttgc ccggtaaaga agcgcccaaa ttcatgtgcc gcttcaacaa
    19321 tggcgcgata accggcgatg ttcgccatcg agcttagtgc gtccagcgat tgtgcgcgtg
    19381 agatacgcgg cacagaatcc atcgccatca cggtcacgtt acgttccgca agtttttgca
    19441 ttaattccgg attctgcgca ggccagataa aactcaccag cgttgtccca ggattcagta
    19501 acgcaatttc atcatctaac ggcgcattga ccttcagaat gatctctgac tgccagacgc
    19561 tattcccttc tacaatttca gcgcccgctt gcacaaacgc tttatcgtca aaacttgcca
    19621 gttgacccgc gccgctctct accgcgacgg taaaacccag tttcagcagc tgttccactg
    19681 tttttggcgt tgctgcaaca cgggtttcat tggttaaccg ttctcttggt atgccaattc
    19741 gcatgatatt cccttccatc ggttttattg atgatggttt gcctgtgtca ggagccacac
    19801 aagccgctca tgtacgagct aaatgttact ccgttaaaat aaattagtaa caaacgccct
    19861 ataacgtact gaaaattatg cctgtgatct agcgccaaaa atcagtcttt cggcgtgaac
    19921 tcgcaaaata ttaacgattc agccgtgata gtgggataaa caccttagaa cgccggataa
    19981 agactgataa ttgtcttcga cggtcgggta aaacgagaca tcgccccggc acgaatcact
    20041 acttaacatt aaattaactt atacaattca gttgcttcag tagtaatgat gctgatacgg
    20101 ctgtttttta agcatagacg gtcatttgag caggattaaa attggcttaa ggaatgtgat
    20161 atgaaaaatg acgcagacag ttacaccgtt taaatgcaat aatcagccac gtttctcgtt
    20221 aataacaata ccagtacctg gtttgcgcaa ggcgaaggat tatttttatg aagcttaaga
    20281 acaccctcct ggcgtcggca ctgctttctg ctacggcatt ctccgttaac gcagcaacag
    20341 aactgacacc ggagcaagcg gcagcggtta aaccttttga ccgtgtagtg gttaccggtc
    20401 gttttaatgc tattggcgaa gcggtgaaag ccgtttctcg tcgcgcagat aaagaaggtg
    20461 ccgcctcttt ttatgttgtc gacacttctg attttggtaa cagcggtaac tggcgtgtgg
    20521 tcgctgacct ctataaagcc gatgctgaaa aagcagaaga aacaagtaat cgcgtaatta
    20581 acggtgttgt cgaactgccg aaagatcagg ctgttctgat tgaaccgttt gacacggtca
    20641 ccgtccaggg cttctatcgt agccagccag aagtcaatga tgccatcacc aaagcggcaa
    20701 aagcgaaagg tgcctactct ttctacatcg ttcgtcaaat cgatgccaac cagggcggca
    20761 accagcgtat tactgcattc atctataaaa aagatgctaa gaaacgtatc gtccagagcc
    20821 cggatgtgat cccggcagat tccgaagcag gacgtgcagc tctggctgcc ggtggcgaag
    20881 ccgcgaagaa agttgagatc ccgggtgttg cgactaccgc atcaccaagt tctgaagtcg
    20941 gtcgcttctt tgaaacccag tcatcaaaag gcgggcgtta caccgtcacg ctcccggatg
    21001 gcactaaagt cgaagaactg aacaaagcga cagcagcgat gatggtcccg ttcgacagca
    21061 tcaaattctc tggcaactac ggcaacatga ccgaagtctc ctatcaggtt gcgaaacgtg
    21121 ccgcgaagaa aggtgctaag tattaccaca tcacccgcca gtggcaggaa cgtggtaata
    21181 acctgaccgt cagcgcagat ctgtataaat aactattcag ttactcctat aaggcggctt
    21241 gatgccgcct ttcgcatttt tagtcacaaa tttccccgcg accattgcat gcagacacat
    21301 ctcttcgtaa aatcccgcgc ttcagtggca aactccattt ttatgcgttt tgccctaata
    21361 tttattcttc ttatcacgtt ttaatcactg gatatcgatg gaaaagaaac tgggactgag
    21421 cgcactcacc gcgctggtat taagctcaat gctgggcgcg ggtgttttca gtctgccgca
    21481 aaatatggcg gcagttgcca gcccggcagc actgctcatc ggctggggta ttactggcgc
    21541 tggcatttta ttgctggcct ttgccatgct gatcctcacg cgcattcgtc ccgaacttga
    21601 cggcggtatc tttacctatg cccgcgaagg gtttggcgag ctaatcggct tttgttccgc
    21661 atggggatac tggctgtgcg cagtcatcgc caacgtctcc tatctggtga tcgttttttc
    21721 cgcgttaagc ttttttacgg acacgccgga attgcgcctg ttcggcgatg gtaatacctg
    21781 gcaatcgata gtcggtgcat cggctttatt gtggattgtt cactttttga tcctgcgcgg
    21841 ggtgcaaacc gctgccagca ttaacctggt ggcgacactg gcaaaattgt tgccgctggg
    21901 tctgtttgtt gtgctggcga tgatgatgtt caaactggat accttcaagc tcgacttcac
    21961 cggacttgca cttggcgtac ccgtgtggga acaggtgaaa aacaccatgc tgatcaccct
    22021 gtgggttttc attggtgtgg aaggtgccgt ggttgtttct gcgcgtgcgc gtaataaacg
    22081 tgatgttggc aaagcgacac tgctggcggt tctctccgct ctgggcgttt acttgctggt
    22141 aacgctgctt tcgctgggcg tggtggcacg tcctgaactg gctgaaattc gtaacccgtc
    22201 aatggccggt ctgatggtgg aaatgatggg accatggggc gaaatcatca tcgctgccgg
    22261 tttgatcgtt tccgtttgcg gcgcgtactt aagctggacc atcatggcgg cagaagttcc
    22321 gttcctggct gccactcata aagcattccc acgcattttt gcgcgccaga acgcacaagc
    22381 tgcgccatcc gcctcgctgt ggttgaccaa catctgcgtg caaatttgtc tggtactcat
    22441 ctggctgacc ggttccgatt acaacacgtt gctgaccatc gcctcagaaa tgattctggt
    22501 gccctatttc ctggtcggcg cgttcttgct gaaaatcgca acccgtccac tgcataaagc
    22561 ggtaggtgtc ggtgcctgca tttatggctt atggttattg tatgcctctg gtccaatgca
    22621 cttgctgtta tccgttgttc tgtatgcgcc ggggctgctg gttttcctgt atgcgcgtaa
    22681 aacgcataca catgataacg tactgaaccg ccaggaaatg gtgctaattg gcatgctgtt
    22741 aattgcctca gttccggcga cctggatgct ggtgggataa cgttcatccc atcgttggtt
    22801 agcaaaggag ataacgatgg gtaaagccca gcccttgcca atattaatta ctggcggtgg
    22861 tcgtcgcatc ggcctcgccc tcgcatggca tttcattaat caaaagcaac cggtgattgt
    22921 cagctatcgg acacactatc cagccattga tggactgatt aatgcaggtg cgcagtgtat
    22981 tcaggctgat ttttcgacca acgacggtgt gatggcgttt gccgatgaag tactaaaaag
    23041 cacccatggt ctgcgtgcta ttttgcataa cgccagtgcg tggatggcgg aaaaaccggg
    23101 tgcgccactg gccgacgtac tggcttgcat gatgcagatc cacgttaata ccccatacct
    23161 gctcaaccat gcgctggaaa gattactgcg tgggcacgga cacgccgcca gcgatatcat
    23221 tcactttacc gattatgtgg tggagcgcgg tagcgacaag catattgcgt atgctgcaag
    23281 caaagcggca ctggataata tgacccgctc gtttgcccgc aagctggcac cggaagtgaa
    23341 agtgaattct attgctccat cgctgatcct gtttaatgaa catgatgatg ccgaatatcg
    23401 acaacaggcg ctgaataaat cactgatgaa aaccgcgcct ggcgagaaag aagtgatcga
    23461 cctggtcgat tacttactta ccagttgctt tgtcaccgga cgcagtttcc cacttgatgg
    23521 cggtcgtcat ctgcgttaat gcagctttat ccagcatata atcaacaccc atgcgcttat
    23581 cccccaacac gcaacagaac caacaaaagc ggcgggcaag cgcatcattc cggtgaaata
    23641 ccacagcgac accaggtaaa caaaataggg aataatcgac cacatactaa aaatgatggt
    23701 tgcgcgtaag gcttcaatgc cgcgttcgct ggcaacaata taatgcgcga taagcgcaaa
    23761 ggtcgggaaa agtggaatca gcccggcgat ataataattt ttcgtttttg ctaaaacacc
    23821 aatcaacagc actaccagcg caccgagcgc ggctttgatt accagcccca tttttttacc
    23881 ttaacacttc cataacaagt catcagtaga atacctgatg aaaacttgtt tagaaacgat
    23941 tgatagtaag taaaaacagc gcggtgtatt gtgacgtttt tatatctacc gtgaatgtta
    24001 tgaacactat cgtatttgtg gaagatgatg cggaagtcgg ttcactgatt gccgcgtacc
    24061 tggcaaaaca tgatatgcag gttaccgtag agccgcgcgg cgaccaggcc gaagaaacca
    24121 ttttgcgaga aaatccggat ttggtgttac tcgacatcat gctaccaggc aaggacggca
    24181 tgaccatttg tcgtgattta cgcgcaaagt ggtctggacc gattgttctt ctaacctctc
    24241 tcgatagcga tatgaaccac atcctggcac tggaaatggg tgcctgcgac tatattctca
    24301 aaacgacgcc ccctgctgtt ttgctagcgc gtttacgttt gcatttgcgt cagaatgagc
    24361 aagccacact gagcaaaggt cttcaggaaa cgtctctgac tccctacaaa gccctgcatt
    24421 tcggcacgtt gaccatcgat cccatcaacc gcgtagtcac cctggctaac actgaaatct
    24481 cgctctcgac agctgatttc gaattattgt gggaattagc tacccatgcc gggcaaatca
    24541 tggaccgcga tgcattgctg aaaaatttac gcggcgtcag ttatgacgga ctggatcgta
    24601 gcgtggacgt ggctatttcg cggttaagaa aaaaactgct cgataacgcc gcagaacctt
    24661 atcgcattaa aactgtgcgt aacaaaggct atctttttgc gcctcatgca tgggaataag
    24721 cgatgaaaaa actgtttatc cagttttacc tgttattgtt tgtctgcttc cttgtgatgt
    24781 ctctgctggt tgggttggtg tacaaattta ccgccgaacg cgcgggcaaa cagtcgctgg
    24841 atgatttgat gaacagttcg ctgtatctga tgcgcagcga attgcgtgag atccccccac
    24901 acgactgggg taagacgctg aaagagatgg atttaaatct ctctttcgat ctgcgtgtcg
    24961 agccactgag taaataccat cttgatgata tttccatgca ccgactgcgt ggcggcgaaa
    25021 ttgtcgccct ggacgatcag tacacgtttt tgcagcgtat cccgcgcagc cactacgtgc
    25081 tggcagttgg tcctgttcct tatctttatt atctccatca gatgcgattg ctggatatcg
    25141 ccctgatcgc ttttattgct atttccctcg cctttccggt gtttatctgg atgcgtccgc
    25201 actggcagga tatgttaaaa ctggaagcag cggcgcaacg atttggcgat gggcatctca
    25261 atgagcgtat ccactttgat gagggttcga gctttgaacg acttggtatc gcatttaacc
    25321 agatggcgga caatatcaac gccttgattg ccagcaaaaa acagcttatt gacggtatcg
    25381 ctcacgaact gcgaacaccg ttagtgcgcc tgcgttatcg actggagatg agcgataacc
    25441 tgagcgccgc cgaatcccag gcgttgaatc gtgatatcag tcaacttgaa gctttaattg
    25501 aagagctgct gacttatgcc cgactcgatc gcccacaaaa cgagcttcat cttagcgaac
    25561 cagacctgcc gttgtggctg tcaacgcatc tggcagatat tcaggcagta acacccgata
    25621 aaacggtacg gataaaaacg ctcgtgcaag gccattatgc ggcgttggat atgcgcttaa
    25681 tggagcgcgt gctggataat ttgctcaata acgccctgcg gtactgccat tcaacggttg
    25741 aaaccagcct gctactgtcg gggaatagag cgacattaat tgttgaggat gatggcccag
    25801 ggattgcccc agaaaaccgc gaacatatct ttgaaccttt tgttcgcctc gatcccagcc
    25861 gggatcgctc aaccggcggc tgcgggctgg ggctggcaat tgtccactct atagcactgg
    25921 caatgggcgg tacggttaat tgtgacacca gcgaactggg tggtgctcgc ttctcgttta
    25981 gctggccgtt atggcataac atcccgcaat ttacctctgc ctgacactac gcgcacgatg
    26041 gtcaagtcac cacgactgtg ctataaaata agtatgttgt aactaaagtg gttaatatta
    26101 tggcgcgtta cgatctcgta gaccgactca acactacctt tcgccagatg gaacaagagc
    26161 tggctacatt tgccgctcat cttgagcaac acaagctatt ggttgcccgc gtgttctctt
    26221 tgccggaggt aaaaaaagag gatgagcata atccgcttaa tcgtattgag gtaaaacaac
    26281 atctcggcaa cgacgcgcag tcgctggcgt tgcgtcattt ccgccattta tttattcaac
    26341 aacagtccga aaatcgcagc agcaaggccg ctgtccgtct gcctggcgtg ttgtgttacc
    26401 aggtcgataa cctttcgcaa gcagcgttgg tcagtcatat tcagcacatc aataaactca
    26461 agaccacgtt cgagcatatc gtcacggttg aatcagaact ccccaccgcg gcacgttttg
    26521 aatgggtgca tcgtcatttg ccggggctga tcacccttaa tgcttaccgc acgctcaccg
    26581 ttctgcacga ccccgccact ttacgctttg gttgggctaa taaacatatc attaaaaatt
    26641 tgcatcgtga tgaagtcctg gcacagctgg aaaaaagcct gaaatcacca cgcagtgtcg
    26701 caccgtggac gcgcgaggag tggcaaagaa aactggagcg agagtatcag gatatcgctg
    26761 ccctgccaca gaacgcgaag ttaaaaatca aacgtccggt gaaggtgcag ccgattgccc
    26821 gagtctggta caaaggagat caaaaacaag tccaacacgc ctgccctaca ccactgattg
    26881 cactgattaa tcgggataat ggcgcgggcg tgccggacgt tggtgagttg ttaaattacg
    26941 atgccgacaa tgtgcagcac cgttataaac cacaggcgca gccgcttcgt ttgatcattc
    27001 cacggctgca cctgtatgtt gcagattaac gcccggcttt catactgccg accatctgtt
    27061 ctggccgtac ccagctgtca aactcggctt cgctaagata ccccagcgca agggccgcag
    27121 cttttaaggt cagcccttct ttatgcgctt ttttggcgat ctccgcggct ttgtcataac
    27181 caatgtgggt gttaagcgca gtcaccagca tcagcgattc attgagtaat tgattgattc
    27241 gctcacaatt cggttcaata cccactgcgc agtgtttgtt aaaactttcc atgccatctg
    27301 ccagcaagcg caccgattgc aggaaattgt ggatcaccat tggacggaag acgttcagtt
    27361 caaagttacc ggaagcgccc cccatgttga tcgccacgtc gttccccatc acctgacagc
    27421 agagcatggt taatgcctca cactgtgttg gattcacttt ccccggcatg attgagctgc
    27481 ccggctcatt ttccgggatt gagatttcac caattccgca gcgcgggcca gaggccagcc
    27541 agcggacatc attggcgatt ttcatcagtg acgcagccaa ccctttcaac gcgccgtgcg
    27601 cctgaaccag ggcatcacag gtcgccagcg cttcaaattt gttcggcgcg gtaacaaacg
    27661 gtgcacaggt aatgactgcc agttcatctg ctacgcgacg cgcatactcc ggatgggtat
    27721 ttagtccagt acccaccgct gtaccgccaa gagccagttc cgctacgtga ggcaggctgt
    27781 attcgatatg tttgagatta tgctcgagca tcgctaccca gccggaaatc tcctgcccca
    27841 gtgttagcgg cgtggcgtcc tgcaagtggg ttcgaccgat tttgacgata tcggcaaatg
    27901 cacgcgattt ttcactcagc gtctgtgtca gggttttaag ctgcggaatg agttgcttgc
    27961 gcagtgccag cagcgccgca acgtgcatcg ccgtcggaaa gacatcgttg gaactttggc
    28021 ttttgttcac gtcgtcgtta gggtgaactt tacgttccat cccgcgcaca ccgccgagta
    28081 attcactggc ccggttagcc agcacttcgt tcatgttcat attactttgc gtgccggagc
    28141 cggtctgcca gatagccagc gggaattcat cgtcatgctg tcctgcgagt acttcatccg
    28201 ccgcctgccg aatggcgctc gctttctctt cagacaacaa gcctaaatct tcattaactt
    28261 ttgccgctgc acgcttggtt agcgccagcg catgaatcag tgaggtgggc attttctccg
    28321 tcgaaatgcg gaaatgctcc agcgagcgtt gagtttgtgc gccccacagc ttatctgccg
    28381 ggacatcaat cgcccccatc gaatcttttt cgctgcgtac tgtattcatg gcctgctcct
    28441 cacctgatta attttttctt tctgttttgc tttcgttaaa caacttttag ctcactcatt
    28501 atttactatt tgataacaaa tgtttggtct ttcgtgccat gtaaaaaaac cgccccgaag
    28561 ggcggctctg tttatttcac acagcgagtg cactgtgtga gttgtatctg ctggaagaag
    28621 tcatttcctt tatcatccac aaggataaac gccgggaaat cttccacttc aattttccag
    28681 atggcttcca ttcccagttc cggatattca acacattcca ggctcttaat acttccctgc
    28741 gccaatacag cggccggacc accgatactg ccaaggtaga agccgccgtg ttttttacag
    28801 gcatccgtca cctgctggct gcggttgcct ttcgccagca tgatcatact tccgccctgc
    28861 gcttgcagtt gatcgacata agaatccatc cgtccggcgg tcgttgggcc aagagaaccg
    28921 gaggcataac cttccggcgt tttggccgga cccgcgtagt aaatcggatg atctttaatg
    28981 tactgcggca gcccttcacc gttatccatc cgctctttca gtttggcgtg agcaatatca
    29041 cgaccgacga taatcgtgcc gttaagcgat aagcgtgtag agacgggata ctgcgacaac
    29101 tgtgcgagga tctctttcat cggacggtta aggtcaacgc gcaccgcttc gccttctccc
    29161 gctttgcgca gctcttccgg gatatatttg cctggattat gttccagttt ttcgatccag
    29221 atcccctgac ggttgatctt cgctttgata ttacggtcag cagagcagga aacacccata
    29281 ccgaccgggc aggatgcgcc gtgacgtggc aggcgaatca cgcggatgtc gtgagcgaag
    29341 tatttaccac caaactgcgc acccagaccg agattttgcg cttcgatcag caattctttt
    29401 tccagttcca catcgcggaa cgcctgaccg tgctcattcc cttccgttgg cagttcatca
    29461 tagtatttcg cggaagccag tttcaccgtt ttaaggttcg tttctgcaga agttccacca
    29521 ataacgaacg caatatgata cggaggacag gccgccgtac ccagcgtgcg catcttctca
    29581 accaggtaat ttttcagttt ccccggcgtc agtaacgctt tggtttcctg atagagatac
    29641 gtcttgtttg ccgaaccacc acctttggcg atacagagga atttgtattc gtcgccatca
    29701 acggcataaa gatcgatctg cgctggcaga ttggtgccgg tattcacttc tttatacata
    29761 tccagcggcg cgttttgcga gtagcgcaga ttatcttcga tataagtgtt atagacaccg
    29821 cgcgccagcg ccgcttcatc accaccaccg gtccatacac gctgcccttt tttaccaaca
    29881 ataatcgcgg tgccggtatc ctgacaggtt ggcagaacgc ctttcgccgc gatgtcggag
    29941 ttacgcagga attgcagcgc cacatattta tcattttcgc tggtctccgg gtcacgcaga
    30001 atgtcggcca cctgttgttg gtgcgccgga cgcagcataa acgacgcatc atgaaacgcc
    30061 tgacgcgcca acagagttaa cgcttcgggt gcgactttca aaatctcctg cccttcaaat
    30121 tcagatacgc taacgtgttc gctggttagc aggtaatact cagtatcatc ttttttgagt
    30181 ggaaaaggag cctgataatg aaagggtttg tttgacattg ttctctcact tactgcctgg
    30241 tttggttatg ctctgggcgg gtgttccgtt gccctgttaa aagcgagtaa caatatccta
    30301 cacacttttt taacaaaaac tgagactagt acgacttttt gcggctccag gttacttccc
    30361 gtaggattct tgctttaata gcgggattaa tttccacatt aaaacaggga ttgatcatgc
    30421 aaaaactcat taactcagtg caaaactatg cctggggcag caaaacggcg ttgactgaac
    30481 tttatggtat ggaaaatccg tccagccagc cgatggccga gctgtggatg ggcgcacatc
    30541 cgaaaagcag ttcacgggtg cagaatgccg ccggagatat cgtttcactg cgtgatgtga
    30601 ttgagagtaa taaatcgact ctgctcggag aggccgttgc caaacgcttt ggcgaactgc
    30661 ctttcctgtt caaagtatta tgcgcagcac agccgctctc cattcaggtt catccaaaca
    30721 aacgcaattc tgaaatcggt tttgccaaag aaaatgccgc aggtattccg atggatgccg
    30781 ccgagcgtaa ctataaagat cctaaccaca agccggagct ggtttttgcg ctgacgcctt
    30841 tccttgcgat gaacgcgttt cgtgagtttt ccgagattgt ctccctgctc cagccggtcg
    30901 caggtgcaca tccggcgatt gctcactttt tacaacaacc tgatgccgca cggttaagcg
    30961 aactgttcgc cagcctgttg aatatgcagg gtgaagaaaa atcccgcgcg ctggcgattt
    31021 taaaatcggc ccttggcagc cagcagggtg aaccgtggca aacgattcgt ttaatttctg
    31081 aattttaccc ggaagacagc ggactgttct ccccgctatt gctaaatgtg gtgaaattga
    31141 accctggcga agcgatgttc ctgttcgctg aaacaccgca cgcttacctg caaggcgtgg
    31201 cgctggaagt gatggcgaac tccgataacg tgctgcgtgc gggtctgacg cctaaataca
    31261 ttgatattcc ggaactggtt gccaatgtga agttcgaagc caaaccggct aaccagttgt
    31321 tgacccagcc tgtgaaacaa ggtgcagaac tggacttccc gattccagtg gatgattttg
    31381 ccttctcgct gcatgacctt agtgataaag aaaccaccat tagccagcag agtgccgcca
    31441 ttttgttctg cgtcgaaggc gatgcaacgt tgtggaaagg ttctcagcag ttacagctta
    31501 aaccgggtga atcagcgttt attaccgcca acgaatcacc ggtgactgtc aaaggccacg
    31561 gccgtttagc gcgtgtttac aacaagctgt aagagcttac tgaaaaaatt aacatctctt
    31621 gctaagctta ttaaaggctt ataacacctt caggcggcca gtccgcctga tttcatttta
    31681 tggataatca ttatgaataa atcgctggta gcggtaggcg tcattgttgc gctaggcgta
    31741 gtctggacag gcggcgcatg gtatacaggc aagaagattg aaacccaact cgaagagatg
    31801 gtcgcgcagg cgaacgcgca actcaaactg acagctcctg aatccaacct ggaagtgagt
    31861 tatcaaaact atcatcgcgg cgtattcagc agccagttgc aactgttggt gaaacccatt
    31921 gccgggaaag aaaatccgtg gattaaaagc ggtcagagcg tcatcttcaa cgaatcggtt
    31981 gatcatggtc ccttcccgct tgcccagctt aaaaaactga acctgatccc gtcgatggca
    32041 tcaattcaaa ccacgctggt taataacgaa gtaagcaaac cactgtttga tatggcaaaa
    32101 ggtgaaacgc cttttgagat taactcgcgc attggttaca gcggtgattc cagttccgat
    32161 atttcgctca agccactgaa ttacgagcaa aaggatgaaa aagtcgcctt tagcggcggc
    32221 gagttccagt taaatgctga cagagacggc aaagccatct ctctttccgg ggaggcgcaa
    32281 agtggtcgga tagacgcagt taacgaatac aaccagaaag tgcagttgac ctttaataat
    32341 ctgaaaaccg acggttccag cacgctggca agttttggtg agcgcgtagg aaaccaaaaa
    32401 ctgtcactgg aaaaaatgac catttcagtg gaaggcaaag aactggcact gctggaaggc
    32461 atggagatca gcggtaaatc ggatctggtc aatgacggta aaacgatcaa tagccaactg
    32521 gattactcgc taaacagcct gaaggtacag aatcaggatc tgggcagcgg caagctgact
    32581 ttaaaagtcg gccagattga tggtgaagcc tggcatcagt ttagccagca atataacgcg
    32641 caaactcagg cgctgctggc acagccagaa attgccaaca atcccgaact ttatcaggag
    32701 aaagtgacgg aagccttctt tagcgccctg ccgctgatgt tgaaaggcga tccggtgatt
    32761 actatcgcgc cgctaagctg gaaaaacagt cagggtgaaa gtgcgctgaa tctgtcgctg
    32821 ttcctgaaag atccggcaac gactaaagaa gcgccgcaaa cgctggcgca ggaagtagat
    32881 cgttcggtta aatctctgga tgcgaaactg accattccgg tggatatggc aactgagttt
    32941 atgactcagg tagcgaagct ggaaggttat caggaagatc aagcgaaaaa actggcgaaa
    33001 cagcaagttg aaggtgcatc agcaatgggg cagatgttcc gtctgaccac cttgcaggac
    33061 aataccatca ccaccagcct gcaatatact aacggtcaga taacgttaaa cgggcagaaa
    33121 atgccactgg aagatttcgt tggtatgttt gcaatgccgg cattaaatgt tccggtcgta
    33181 cccgctattc cgcagcagta attcacagcc aaaacgccgg agatttttct ctccggcgtt
    33241 attttttact tcagcataaa gtcatactga atactcattt tcacatccca gtaattcagg
    33301 cgatcgccca gcgtactgcc atcgcgcttt tcccgccctt tatcggcagc aagaaagaaa
    33361 ttcatccctt gtaacggccc ttcgctgacc cgatagttca taccacctaa gatacccgtg
    33421 ccttcaatcc gcacatctcg tttactggat tcatagccgc gagtcacaat cggtgcaaat
    33481 gtcagtgtaa attgcggcgt taaacgatag ttgacgccca attgccagga ctgcattcct
    33541 tcacggttac gatcaagact taatgaaaag ggatagccaa tgtcggtatc caccacggca
    33601 ccattcattg catggcgcaa ataaccacca ctgccaaata cgcttcccca cggcgcattc
    33661 caggtcaatt gtccactaac caacgccgtt tcattgggct ggctggtatt acgacttaaa
    33721 ccttcgagct gcgcgtaaaa gccgagcaac tcccctttca acgtataatt cgggtcgagc
    33781 ggtatgcttt tctcgagaat actttgtatc tgattggaaa catctttctg taccgatgcc
    33841 cccagagaat agcgactggt atcatcatgc caactggcgg ctaaaaaaga acgtgcgcga
    33901 tgtttatcgt attttcccgt ctggctgtta tacacccctt catcacgaat accggttgtt
    33961 gaatcagtcc ataaaaatac tcgtgaacgc cacattcccc acagatcaat atttggtgtt
    34021 aaagcccaat aacctaatgc tccttcagaa actgtcggta atgtacgttg cgaactgcca
    34081 gccattaacg gaatacgata aggtaagcta ccataactat aatccgtccc gaagcgacct
    34141 aatttaagca ggctattttc actgggtttt atttttactg cccccagggc aaagccaaaa
    34201 cttttatcac catcaagata ccaccgggta ctcatatcag cacgagcacc taatttataa
    34261 acataatatg ccccgccttc aacgccaata atattggaat aataaccact attgaaatcg
    34321 agcaatcctc cctgtaccca tgcgtaaata tctcttcccg cgccagcact gggcttatcc
    34381 gccctgcgca attcgttttt cagtctgatg cgtagtcccg cttcatcgtc agccagttgc
    34441 gccgcataag cagaggtaag gccagaggca gccgtcaggc aaataacggc catggccact
    34501 atttttctca tagttgcacc tttg
//
LOCUS       DAZKHC020000044        34227 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZKHC020000044 DAZKHC020000000
VERSION     DAZKHC020000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.044"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..198
                     /locus_tag="ACHOSM_RS12185"
                     /old_locus_tag="pgaptmp_002437"
                     /pseudo
     CDS             61..198
                     /locus_tag="ACHOSM_RS12185"
                     /old_locus_tag="pgaptmp_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(306..1541)
                     /gene="aslB"
                     /locus_tag="ACHOSM_RS12190"
                     /old_locus_tag="pgaptmp_002438"
     CDS             complement(306..1541)
                     /gene="aslB"
                     /locus_tag="ACHOSM_RS12190"
                     /old_locus_tag="pgaptmp_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU4558940.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1688..1764)
                     /locus_tag="ACHOSM_RS12195"
                     /old_locus_tag="pgaptmp_002439"
     tRNA            complement(1688..1764)
                     /locus_tag="ACHOSM_RS12195"
                     /old_locus_tag="pgaptmp_002439"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1728..1730),aa:Pro,seq:tgg)
     gene            complement(1807..1893)
                     /locus_tag="ACHOSM_RS12200"
                     /old_locus_tag="pgaptmp_002440"
     tRNA            complement(1807..1893)
                     /locus_tag="ACHOSM_RS12200"
                     /old_locus_tag="pgaptmp_002440"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1857..1859),aa:Leu,seq:cag)
     gene            complement(1914..1989)
                     /locus_tag="ACHOSM_RS12205"
                     /old_locus_tag="pgaptmp_002441"
     tRNA            complement(1914..1989)
                     /locus_tag="ACHOSM_RS12205"
                     /old_locus_tag="pgaptmp_002441"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1954..1956),aa:His,seq:gtg)
     gene            complement(2048..2124)
                     /locus_tag="ACHOSM_RS12210"
                     /old_locus_tag="pgaptmp_002442"
     tRNA            complement(2048..2124)
                     /locus_tag="ACHOSM_RS12210"
                     /old_locus_tag="pgaptmp_002442"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2088..2090),aa:Arg,seq:ccg)
     gene            complement(2227..3612)
                     /gene="yifK"
                     /locus_tag="ACHOSM_RS12215"
                     /old_locus_tag="pgaptmp_002443"
     CDS             complement(2227..3612)
                     /gene="yifK"
                     /locus_tag="ACHOSM_RS12215"
                     /old_locus_tag="pgaptmp_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4558941.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3803..4543)
                     /gene="wecG"
                     /locus_tag="ACHOSM_RS12220"
                     /old_locus_tag="pgaptmp_002444"
     CDS             complement(3803..4543)
                     /gene="wecG"
                     /locus_tag="ACHOSM_RS12220"
                     /old_locus_tag="pgaptmp_002444"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HGU4558942.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4546..5898)
                     /gene="wzyE"
                     /locus_tag="ACHOSM_RS12225"
                     /old_locus_tag="pgaptmp_002445"
     CDS             complement(4546..5898)
                     /gene="wzyE"
                     /locus_tag="ACHOSM_RS12225"
                     /old_locus_tag="pgaptmp_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HGU4558943.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5895..6974)
                     /gene="rffT"
                     /locus_tag="ACHOSM_RS12230"
                     /old_locus_tag="pgaptmp_002446"
                     /gene_synonym="wecF"
     CDS             complement(5895..6974)
                     /gene="rffT"
                     /locus_tag="ACHOSM_RS12230"
                     /old_locus_tag="pgaptmp_002446"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HGU4558944.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(6971..8221)
                     /gene="wzxE"
                     /locus_tag="ACHOSM_RS12235"
                     /old_locus_tag="pgaptmp_002447"
     CDS             complement(6971..8221)
                     /gene="wzxE"
                     /locus_tag="ACHOSM_RS12235"
                     /old_locus_tag="pgaptmp_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HGU4558945.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVIKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8223..9353)
                     /gene="rffA"
                     /locus_tag="ACHOSM_RS12240"
                     /old_locus_tag="pgaptmp_002448"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8223..9353)
                     /gene="rffA"
                     /locus_tag="ACHOSM_RS12240"
                     /old_locus_tag="pgaptmp_002448"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HGU4558946.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9358..10032)
                     /gene="rffC"
                     /locus_tag="ACHOSM_RS12245"
                     /old_locus_tag="pgaptmp_002449"
                     /gene_synonym="wecD"
     CDS             complement(9358..10032)
                     /gene="rffC"
                     /locus_tag="ACHOSM_RS12245"
                     /old_locus_tag="pgaptmp_002449"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HGU4558947.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRISPEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10010..10891)
                     /gene="rfbA"
                     /locus_tag="ACHOSM_RS12250"
                     /old_locus_tag="pgaptmp_002450"
     CDS             complement(10010..10891)
                     /gene="rfbA"
                     /locus_tag="ACHOSM_RS12250"
                     /old_locus_tag="pgaptmp_002450"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGU4558948.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10910..11977)
                     /gene="rffG"
                     /locus_tag="ACHOSM_RS12255"
                     /old_locus_tag="pgaptmp_002451"
     CDS             complement(10910..11977)
                     /gene="rffG"
                     /locus_tag="ACHOSM_RS12255"
                     /old_locus_tag="pgaptmp_002451"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGU4558949.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTAHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIAHELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11977..13239)
                     /gene="wecC"
                     /locus_tag="ACHOSM_RS12260"
                     /old_locus_tag="pgaptmp_002452"
     CDS             complement(11977..13239)
                     /gene="wecC"
                     /locus_tag="ACHOSM_RS12260"
                     /old_locus_tag="pgaptmp_002452"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HGU4558950.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13236..14366)
                     /gene="wecB"
                     /locus_tag="ACHOSM_RS12265"
                     /old_locus_tag="pgaptmp_002453"
     CDS             complement(13236..14366)
                     /gene="wecB"
                     /locus_tag="ACHOSM_RS12265"
                     /old_locus_tag="pgaptmp_002453"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGU4558951.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14422..15468)
                     /gene="wzzE"
                     /locus_tag="ACHOSM_RS12270"
                     /old_locus_tag="pgaptmp_002454"
     CDS             complement(14422..15468)
                     /gene="wzzE"
                     /locus_tag="ACHOSM_RS12270"
                     /old_locus_tag="pgaptmp_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HGU4558952.1"
                     /translation="MTQPMPGKAAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRSMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15480..16583)
                     /gene="wecA"
                     /locus_tag="ACHOSM_RS12275"
                     /old_locus_tag="pgaptmp_002455"
     CDS             complement(15480..16583)
                     /gene="wecA"
                     /locus_tag="ACHOSM_RS12275"
                     /old_locus_tag="pgaptmp_002455"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HGU4558953.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16823..18082)
                     /gene="rho"
                     /locus_tag="ACHOSM_RS12280"
                     /old_locus_tag="pgaptmp_002456"
     CDS             complement(16823..18082)
                     /gene="rho"
                     /locus_tag="ACHOSM_RS12280"
                     /old_locus_tag="pgaptmp_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HGU4558954.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18167..18268)
                     /gene="rhoL"
                     /locus_tag="ACHOSM_RS12285"
                     /old_locus_tag="pgaptmp_002457"
     CDS             complement(18167..18268)
                     /gene="rhoL"
                     /locus_tag="ACHOSM_RS12285"
                     /old_locus_tag="pgaptmp_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HGU4558955.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18409..18738)
                     /gene="trxA"
                     /locus_tag="ACHOSM_RS12290"
                     /old_locus_tag="pgaptmp_002458"
     CDS             complement(18409..18738)
                     /gene="trxA"
                     /locus_tag="ACHOSM_RS12290"
                     /old_locus_tag="pgaptmp_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HGU4558956.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18869..20134
                     /gene="rhlB"
                     /locus_tag="ACHOSM_RS12295"
                     /old_locus_tag="pgaptmp_002459"
     CDS             18869..20134
                     /gene="rhlB"
                     /locus_tag="ACHOSM_RS12295"
                     /old_locus_tag="pgaptmp_002459"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HGU4558957.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20268..21752
                     /gene="gppA"
                     /locus_tag="ACHOSM_RS12300"
                     /old_locus_tag="pgaptmp_002460"
     CDS             20268..21752
                     /gene="gppA"
                     /locus_tag="ACHOSM_RS12300"
                     /old_locus_tag="pgaptmp_002460"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HGU4558958.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21799..23820)
                     /gene="rep"
                     /locus_tag="ACHOSM_RS12305"
                     /old_locus_tag="pgaptmp_002461"
     CDS             complement(21799..23820)
                     /gene="rep"
                     /locus_tag="ACHOSM_RS12305"
                     /old_locus_tag="pgaptmp_002461"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HGU4558959.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24037..24486
                     /locus_tag="ACHOSM_RS12310"
                     /old_locus_tag="pgaptmp_002462"
     CDS             24037..24486
                     /locus_tag="ACHOSM_RS12310"
                     /old_locus_tag="pgaptmp_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGU4558960.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24685..24966
                     /gene="ppiC"
                     /locus_tag="ACHOSM_RS12315"
                     /old_locus_tag="pgaptmp_002463"
     CDS             24685..24966
                     /gene="ppiC"
                     /locus_tag="ACHOSM_RS12315"
                     /old_locus_tag="pgaptmp_002463"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HGU4558961.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25053..26528)
                     /gene="ilvC"
                     /locus_tag="ACHOSM_RS12320"
                     /old_locus_tag="pgaptmp_002464"
     CDS             complement(25053..26528)
                     /gene="ilvC"
                     /locus_tag="ACHOSM_RS12320"
                     /old_locus_tag="pgaptmp_002464"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGU4558962.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26678..27571
                     /gene="ilvY"
                     /locus_tag="ACHOSM_RS12325"
                     /old_locus_tag="pgaptmp_002465"
     CDS             26678..27571
                     /gene="ilvY"
                     /locus_tag="ACHOSM_RS12325"
                     /old_locus_tag="pgaptmp_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HGU4558963.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QALFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(27623..29167)
                     /gene="ilvA"
                     /locus_tag="ACHOSM_RS12330"
                     /old_locus_tag="pgaptmp_002466"
     CDS             complement(27623..29167)
                     /gene="ilvA"
                     /locus_tag="ACHOSM_RS12330"
                     /old_locus_tag="pgaptmp_002466"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HGU4558964.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29170..31020)
                     /gene="ilvD"
                     /locus_tag="ACHOSM_RS12335"
                     /old_locus_tag="pgaptmp_002467"
     CDS             complement(29170..31020)
                     /gene="ilvD"
                     /locus_tag="ACHOSM_RS12335"
                     /old_locus_tag="pgaptmp_002467"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGU4558965.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31085..32014)
                     /gene="ilvE"
                     /locus_tag="ACHOSM_RS12340"
                     /old_locus_tag="pgaptmp_002468"
     CDS             complement(31085..32014)
                     /gene="ilvE"
                     /locus_tag="ACHOSM_RS12340"
                     /old_locus_tag="pgaptmp_002468"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HGU4558966.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDGLMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32034..32297)
                     /gene="ilvM"
                     /locus_tag="ACHOSM_RS12345"
                     /old_locus_tag="pgaptmp_002469"
     CDS             complement(32034..32297)
                     /gene="ilvM"
                     /locus_tag="ACHOSM_RS12345"
                     /old_locus_tag="pgaptmp_002469"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HGU4558967.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32294..33940)
                     /gene="ilvG"
                     /locus_tag="ACHOSM_RS12350"
                     /old_locus_tag="pgaptmp_002470"
     CDS             complement(32294..33940)
                     /gene="ilvG"
                     /locus_tag="ACHOSM_RS12350"
                     /old_locus_tag="pgaptmp_002470"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HGU4558968.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEILEKLS"
     gene            complement(33943..33993)
                     /gene="ilvX"
                     /locus_tag="ACHOSM_RS12355"
                     /old_locus_tag="pgaptmp_002471"
     CDS             complement(33943..33993)
                     /gene="ilvX"
                     /locus_tag="ACHOSM_RS12355"
                     /old_locus_tag="pgaptmp_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HGU4558969.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34080..34178)
                     /gene="ilvL"
                     /locus_tag="ACHOSM_RS12360"
                     /old_locus_tag="pgaptmp_002472"
     CDS             complement(34080..34178)
                     /gene="ilvL"
                     /locus_tag="ACHOSM_RS12360"
                     /old_locus_tag="pgaptmp_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HGU4558970.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8273 a         9459 c         8659 g         7836 t
ORIGIN      
        1 tctcaattat caatgcttaa ttacgtcatc attttgatga catgaaaccg gagcatttgc
       61 atggaatttt cgttttcacc caaacttctt gttgttgctg tcgccgccgc tcttcctctc
      121 atggccagcg cagcagatac cccgtcaact gccaccgcac gcaaaggctt tgccggatac
      181 gatcacccaa accagtatcc acagaacgct gcaatttatt gaatttgcac gatcatgtag
      241 gccggataag gcgtttacgc cgcatccggc aatcaaccgc aggcggccac aaaatggccg
      301 cttttttact tattcaccac cagcaaatgt gcctgcataa tgtcactggc cgggcgaccg
      361 tgcgccagca aatcagccat tgctttaaga tatggcggta gatggcggaa ataacgctga
      421 tacccggcac acaaataatt cagccccggt ttgccgctgg catcgagcat gaagcggtgt
      481 ttcgggcagc ctccccagca cgcttttaac acgttacaac tgcgacactg cgccggtaat
      541 tgcttaaatt tatcttcacc aaacacctgc tgttgcgggg aatcgatcat ttctgcaatt
      601 gtttgctgat gcatattccc cagccgatat tgcggataaa catagtgatc gcaggcgtaa
      661 acgtcgccgt tgtgctcaac aatcaccgag cgcccacagg ttggctgatg atggcaaacc
      721 gcacccggcg caccgacaaa attggcaaac gcccattcga tattcatcac gaaaatcttg
      781 ccgacgtcgc gtttgatcca gtggtcgaat atcgccacca gaaattcacc aaattcatcg
      841 gggcgcaccg accattccgt cagttccccc tgaatatcac caggcgcatg caatttcagt
      901 ccatcgcggg cagctgtttc atcagccaga cgctcgacca ccggaataaa ctggatgaat
      961 tcgactcccg catcgcacaa aaaatcataa acctgcaacg gttgctgcgc gctggtgcgg
     1021 ttgacgcaga ccagcacgtt atagtcgaca tgatgttttt gcagcagcgt cagggcacgc
     1081 atcaccagct tatgcgttgg tctgccacct ttggtcacgc gatattgatt gtggatctca
     1141 gccggaccgt ccagcgataa cccaacaaga aaatgatttt ctgccagaaa tgcacaccat
     1201 ttatcatcga gcagcacgcc gttagtctgg aagctgttac ttatcttcct gccagcaccg
     1261 tatttcgcct gtaactttac ggcacggcgg taaaaatcca gcccgagtaa cgttggttcg
     1321 ccgccctgcc aggtaaaagc cacttcgttt tgcggttcgc tggcagcgat atagtgacgg
     1381 acatacgctt ccagcgtgtc atcgtccata tgcgtgactg gcttttcgcg gtaaagggat
     1441 tgtttttcga gataaaaaca gtagtcacag ttcagattac aatcggaacc actcggtttc
     1501 gccatcacat gaaaagcacg cgttggaacc tgttgcagca tggtcactcc ttgcctgaag
     1561 tcatccttca atacgttaag ggcgaaatca taccgtgggg caatgagtta ttacgggcgt
     1621 ttattgcgaa gggagggtga cgaaatgcac agaaaacaaa aaaccccgcc gaagcggggt
     1681 tcaaaattgg tcggcgagag aggattcgaa cctccgaccc actggtccca aaccagttgc
     1741 gctaccaagc tgcgctactc gccgaaatac tgctttttga atttttagtt caattcttta
     1801 aagtcgtggt gcgagggggg ggacttgaac ccccacgtcc gtaaggacac taacacctga
     1861 agctagcgcg tctaccaatt ccgccacctt cgcattgtca caacttctaa taatggggtg
     1921 gctaatggga ttcgaaccca cgacaactgg aatcacaatc cagggctcta ccaactgagc
     1981 tatagccacc actacaaatc ttgttacgcg gtattactac caccgcagct caagcgccgg
     2041 gactaaatgg cgcgcccgac aggattcgaa cctgagacct ctgcctccgg agggcagcgc
     2101 tctatccagc tgagctacgg gcgcttagcg ccgttgcggg ggtggataat acggacttcc
     2161 cacccctctg tctagtccct ttttaaataa attgcgcgtt tggttacggt ttgtgcgttt
     2221 tgctgcttat tcctccagtt tatgcgcttt cccgtggcga ttaaggccaa aaactttata
     2281 aatcgccgtc accgccagca taaagatgat gccaacaaac agcgacatac gcgtatcttc
     2341 attaaagtac atgccgatca aaacgcaaat caggaatgcc attgttacgt agttggccca
     2401 cgggaacagg attgagcgga acggatggct ggcaatcgcc gctttatgtg cacgccgaaa
     2461 acgcagctgg cttatcaata tcacaaacca tggcaccatc cccggaagca cgctggcact
     2521 gtagacgtag acaaacacac gctgcggatt gggaatgatg tagttcaggc atgagccaat
     2581 tagcagaata gcaatagata ctgccacacc cgcaactggt acgccgtgac gggaaacttt
     2641 cgccatcgcc gccggtaact gacggttttt cgccagtgcg tagagcatac gtccgcaact
     2701 gtacatgccg ctgttacagc cagagagcgc agccgtcagc accacaaagt tgataatgcc
     2761 cgccgctgcg gtaataccga ttttggcaaa agtcagtacg aacgggctgc cgttgctgcc
     2821 aatttcattc cacgggaaga tggtgacgat aacgaaaatc gcgcctacgt agaaaatcag
     2881 gatccgccac agcactttgc ctacggcact gcgcagcgtc acctgcggat tcttcgcttc
     2941 accggcagta atgccaatca gctccacgcc ctggtaggac gccaccacaa tacacagcgc
     3001 ggtcaggaac cctttccagc cacccgcaaa gaaaccgcca tgctctgtga gattgctaaa
     3061 accaatcgac tgcccgccat tgccaaagcc aaagaaaatc acgcccaggc caatgacaat
     3121 catcacgata atcgtggtga ctttgatcat cgcgaaccag aactcgattt cgccgtacaa
     3181 ccgcaccgcc gccagattcg ccaacgccac cagcgccact gcgatcaatg cgggtatcca
     3241 ctgcgccatc tccgggaacc agaactggac ataaacgcca atggcggtga tttctgagat
     3301 ccccacggcc atccacataa accagtaaga ccaggcggtg agataaccaa aaaacgggct
     3361 catataacga tgcgcataaa cggcgaacga accggtaacc ggttcgagga acaacatttc
     3421 gcccattgaa cgcatgatga aaaagacgaa tagcccggcg atgatatagg ccaacaatac
     3481 ggatggcccg gcccatttca aggtactggc ggcccccata aacaggccga cgccaatggt
     3541 gccccccagg gcgatgagtt cgatatgtcg agcttccagc ccacgctgta gctctggttt
     3601 gttatctgcc ataaatcctc gtgttgtgtt tgcatgcttt ccggtgttac cggttatcgt
     3661 tatgggtaca tcgagtgttg cgaatgtttt cgtaattcag gagaaatggc aaataaagca
     3721 ttaaaaattt gtatgctttg tgtaataaaa aagcagacag gcgacggagt gaccactccg
     3781 tcgctttaca aagagaggaa aatcagaggt tgccggtgta gtgccagcgt aaataacgca
     3841 gcaaacgaag ctgacgctta atgcggctcg gctgcgaaag caggcggtag agccactcca
     3901 gtcccagcgt ttgccagatt ttcggtgcgc gttttacgtg accggtgaaa acatcgtaag
     3961 tcccgccaac gcccatatac agcgcatctg gatgcaccag acggcagtcg cgcatgaaga
     4021 tctcctgctt tggcgatccc atcgcaacgg tgacgatttg cgctccgctg gcatgaatgc
     4081 gttcaaacag cgcctgacgc tgctcgggtt taaaataacc atcctgactg ccaacgatat
     4141 tcacattcca ttggttgcgc agtttagctt cagtttgcgc cagcacttca ggtttaccgc
     4201 ccacaagaaa taccggcgtc ccttctttgc ctgcgcgcgc catcagctct tcccagagat
     4261 cggcaccggc aacgcgggaa acctgcgcct gcgggtactt tttacgtact gaacgtacaa
     4321 cgctgatgcc atccgcatat ttaaattcgg cagcgttaat taactccctg acctcggcgt
     4381 tatcttcaat ggtcagcatt ttttcggcat taatggcaac cagcgttccc tgcttaagct
     4441 gcccgtcagc aaacagataa tcgagggcgt gctgcatatc acgccaacca atcaactgta
     4501 agccacgcag cgtataggtc ggtgccgtgg tgttgttatt cattgttatc cttcaacctg
     4561 cgtccggagc gatgattttg tacgtttatg aatgagtccg gcgctttcaa aaagccagta
     4621 caacagtttt gcgatcatca gacatgcgcc gaagaccacg ataaaaaaga ctacgcgtga
     4681 gacaaacgaa tccagccctt cacgtgccag cacgatcata ttgaagatcg ccccaaagca
     4741 gaaactgtgc aatatcgcag ccttatagcg attaggctcg cggttgccca gctcatacag
     4801 ccagtcgaac catttgatga tcagaccaac cacgatcgcc ccgagcggga tgaacaacgc
     4861 gccgcccata acaaccagtg agcctataag cgtaggcgag atcgccagtc cggagtggtt
     4921 attcagcact tcccaggtaa agtagttggc tgagttcagc accatactcg ggcgacccgg
     4981 ccacagccag gaagggataa agacatagaa atcgcggaca attggagcca ggccctggaa
     5041 gtcgatgttg tcgtagttct gcaacagcaa cgccagattc tcccacggcg agaaggtgtc
     5101 gcgagtgaga tagagaaacg tatagaacgc ttcatcgccg ctcacattca ttccatagcg
     5161 ttttagcgcc agccagaaca tgcccacaat ccccagcacg cccgccgccg ccagcatcca
     5221 caacgaaatc cagccgcgaa taatgccaat aaacaggaag atagcgaatg cgatgatgat
     5281 attggcgcga gtgccgccga caatcatata agtcagcaag ccaaaggcga ccgtgctgac
     5341 gaggaaaaac agccacgctt tgctatcctg gcgcagaaag tagaccacca gcatcgccgg
     5401 gatgaaaaag taaaagaagc gttttaacgc cacgccggag acttcactgg agaagatctg
     5461 actgtaggag ttaagccgga acagcaaaaa gccgttgtgc atgaagaaga tgccgacgct
     5521 taccagcgcg atgcccatca ggatcaccca cgtaagattg gtctccacgc ggttcatggt
     5581 aaacagcgga cggcgcggta catcagcaac gcgtttgcgt aggcgggttt tgtaggtgac
     5641 atagtaaacc gcgtagaagc agcccgcaga aagcaacgcc tgcaacaaga tttctggcgg
     5701 cgcgacacca acatcaaagc gaaataccag cacgctggtc agcgggaagc cgaagaaaaa
     5761 ggtgagcaaa aacagcaatg aaaagaagac attgaagtta aagcgcacac ggcgaaactc
     5821 aaaccaggtc agcgtggcaa taaacagcgt gcagagcagc cagaccacaa acaggccact
     5881 gaattgcagc agactcatgc gccctccccg gcggcaatcg ccaacgcccg ctgccagcct
     5941 tgtagatagt tagggctaaa gaaggcaatg gtgtttttat ccaccgacgc caactggcgc
     6001 tgcgcttcac gcacaatatc ctcgttgaga tcgtcggtag taaacagcac tggcaaatgc
     6061 tgttccgtca tatcctgcca gaacggattt tcccggttaa gcacacaagg aatgcccgcc
     6121 tgaatcagta agcacagcgt accaatgccc tgctggcgag caaaaataaa gtaaccaaga
     6181 tcgcactgac gaagtagcgc cagataggcg tcaaattcca gtttttcgct caaaatttgc
     6241 agattttctt cgctgaataa ctccagcccc gcctgacgaa cttcctcaat gtacgcttcg
     6301 ttattaggcg gatatcccat cggcaccacc acttttaccg tatcgccaaa ttgctgatga
     6361 acggcacgta aagcggcgac atgctcattg ctgaggtcgc cggagttccc caccagaatg
     6421 gtcatttttc cttcacgttg ccgatcgttc gccatcgtat tgagagaagg gtccatccgc
     6481 gtcgggaagt acagcagttc tccccgcacc tttgggtgcg ttttggcaaa aaagctcaaa
     6541 tcgccgcggg tggcaaatac acagccgact cgcttttgcg ccaggcgacg tagtgggtaa
     6601 aaaagcttat atctcaagcc actggaaagc tcatacaggt ctgcccccca gatatgccag
     6661 aaaaactggc tgggcttaat gccaccactc agcagagcca gccacagtgt gggattgaac
     6721 tgaccgtgga aaaaaaatcg ctgctgacgg ttagcttttg ctttcgcgat gaccgcttcc
     6781 gccagcgatt ttttcccagg gaaaaattgc acagaaagcg ccggacagct atcacttaag
     6841 ccgtcgtcct tgccaacaac cataaactcg cgcgcgtgct cgctcgtcgc agccagcgcg
     6901 tcattgaaaa accgcaaaac ggttcggtta tggtgaggga tatccgatcc cagtacgtga
     6961 atcagtacag tcatgcccgc ctacgccaga gtaaaaacac gccacaacaa agagaaaaat
     7021 agacgatata agttgccata tatgcctgcg ccgcgcccag tgcaccatgc gcagggatta
     7081 gccagtgggc aaataccatc aataaagtga actggctgat ttccgccaga atataaaacc
     7141 gcaatgacgc tttggcgatc accagataac caaagacata agcgcccact tttaacacat
     7201 cacccactaa ctgccaggca aagagatcgc gcatagcggt aaatttattc gacaacagca
     7261 gccagatagc aaaatcacgc agcagccaga cggtaaaact cgccgccgcc actgccggta
     7321 agacgaattt tagcgattta atcacttccc gggtgatatc gcgcttttcc gttagccgcg
     7381 acaacgtggg cagcaaatat acgctgaacg atgccgtaat aaattgcagg taggcatcgg
     7441 aaatactgct caccccttgc cagatcccca cctcatccca gctatactgc gccgccagca
     7501 gtttacgcat catgatgtaa gcaacaggca aggtcaccga cgtaatcaac gccatgagcg
     7561 taaatttgct caactgccct gccagaccgt tatcccagct gggtttcaga tagcttaacg
     7621 ggatgacacc acgtttgatc aacatgatgg cggcaggaat caccaccagc gcgggaatca
     7681 gcgccagacc cagcaacgcc ccttcataac cgcccaaacg gtaactgacg tagtacgcga
     7741 gaacgccaat caagctgccg acaatcagcg ataacgcatt acctgcggca tcgcgaaagc
     7801 ctttcatcag cgccagtaac aggttgcccc aggcgatccc catttgcacc agcgccacta
     7861 aacgcaccag cccctgatag tcggtattac caaacaatcc ctggctgatt ggcgcagctg
     7921 ccagcacaaa aaccagcgcc atcagcgtag agaaaccaag taccatcgct gatgaagtgc
     7981 cgaccacgcg gcgcagctgt tgcggattat catggtactg ggcaacgtat ttggttacac
     8041 cgttaaagat gccagctccg gcaagcacgc cgagcacggt aatcaactgg cggaaatttg
     8101 ccgccagccc aagccccgcc ggaccaaatg acacagccag caacttaccg accagtaacc
     8161 cggcaccaat cttgaccagt gtactggccg ccgtccacaa ggacgctttt gccaacgaca
     8221 tatcaggaga agtagttcaa caaagtcgca attaccgtac gctgattgac gggcgacagg
     8281 ttgtagaaca gcggcaggcg cagcaggcgc tcgctctctt tggtggtgta gcgatcttca
     8341 ccgtggaact caccaaagcg ttcccccgca gggcaaccgt gcagcggaat gtaatggaac
     8401 accgccatga tttccgcttc tttcagaaag ttaatcaacg cgctccggtc atcaatatcc
     8461 cgcagtttaa tgtagaacat atgcgcgttc tgcacgcagc catcgggaat cgacggcagc
     8521 tcgatacgcc cggctttcgc cagaggcgct aacgcatcgt agtagttttg ccacagcgcc
     8581 agacgttgct ggttgatacg atccgctgct tccagttgcg cccacaggta cgcagcttgc
     8641 agatcggaca tcaaatagct ggagccaata tcgcgccagg tatatttatc gacctgacca
     8701 cggaagaact ggctgcggtt agtgcccttt tcacggatga tctcggctcg ttcgattaac
     8761 gctttatcgt taatcagcgt cgcgccgcct tcaccacccg ccgtatagtt tttggtttca
     8821 tggaagctaa agcagccaat atgaccaatg gttcccagtg cacgcccttt gtaagtggac
     8881 atcacgccct gagcggcatc ttctaccaca aacagattat gctttttcgc caacgccata
     8941 atggtgtcca tttcgcaggc cacacccgcg taatggaccg gcacgataac gcgcgttttg
     9001 tcggtgatcg ccgcttcaat cagcgtttcg tcgatgttca tggtgtccgg gcgaacatcc
     9061 acaaaaacga tttttgcgcc acgcagcaca aaggcattgg cggtggagac aaaggtgtag
     9121 ctcggcatga tcacttcatc gccaggctgg atatcgagca gcagcgccgc catctccagc
     9181 gaagcggtgc aggacggcgt cagtaacact ttggcgctgc caaaacgttg ctccagccac
     9241 tgctggcagc gacgggtaaa accgccatcg ccacacagtt tgccgctacc cattgccgac
     9301 tgcatatagt cgagttcggt tcccaccacc ggcggtgcgt taaatggaat catgtgatca
     9361 cctgtataac cagtacgcgg tgctttctac attcgcacca ctttgtatgt atcgtttaag
     9421 cgcggcggtg ttgcccattt gggtcgccac ccgcaaagtt gttttaccgc gagcatacgc
     9481 ccagtttagc gccgtttgca tcagctcagc acctgcaccg cgtccagcca gcaggccaat
     9541 tcgcgcatct gtcgcattga gttcccgtaa agagacataa ccgcgaatat cgccggacgc
     9601 cgcacgtaaa atcagacatt gatgatcaaa ggtgccgcgc acggcatttt caatccactg
     9661 tgcataaaag cgaccgctgg cgtcaggcgc ataccacggc gcacgaaaac ggctttgcgc
     9721 aaatgcggcg ctggctaact gacgtaatgc gggaatatcg gtctcttgtg ccactacagc
     9781 accactatca ctgacattgt tcacgggtag cgccaaatca acttcacctt ctaccaggga
     9841 gaatcccagc tgttgcaggg catccagttc acccgtattt gatgccgcaa ttttggcctg
     9901 cacccgtgac cacggcgcta acacgtctgg cgtcaggagc ggtgcttcag gactaatgcg
     9961 cacgatggcg ctgttaacac caaagaaggc gttttcccag gttagtggct caatactggc
    10021 gcggacgggc acgaagtaac tccagcagat attggccgta gccagttttc gctaatgaac
    10081 tggcagcacg cttcacaccc tcgtcatcga gccagccgtt acgccaggcg atctcttcca
    10141 ggcaggcaat cttaaagccc tggcgttttt ccaccgtctg cacaaaggta ctggcttcaa
    10201 tcaggctgtc gtgagtgcca gtgtccagcc aggcaaatcc gcgcccgagc agttcaacgg
    10261 tcaggttgcc cgcctcgagg tacatctggt tgatggaggt aatctccagt tcaccacgct
    10321 ccgacggctt cacctgcttt gcgtactcca cgactttact gtcgtagaaa taaagcccgg
    10381 tcaccgccca gtttgatttc ggctgttttg gcttttcttc cagcgagata gcgcggaaat
    10441 tgtcgtcaaa ctccacgacg ccaaagcgtt ccgggtccat cacctgatag ccaaataccg
    10501 tcgccccttc cgtgcgcgcc gcaacatgac gcagcttcgg actgaagccc tgaccgaaga
    10561 agatgttatc gcccagcacc agacaagaag gttcaccatt aaggaaggtt tcaccgatga
    10621 taaaggcctg cgccagaccg tccgggctgg gctgttcggc atattccagc tggataccga
    10681 actcactacc atcgcccagc aggcgctgga aataaccttt atcttccggc gtagtgatga
    10741 tgagaatttc gcggataccg gccagcatca gcaccgacag cggatagtaa atcattggct
    10801 tatcgtaaat cggcaacagt tgcttcgata cgccgcgcgt aatcggatgc aatcgggtgc
    10861 cggaaccgcc cgccaggata atacctttca tgtttgcctc cgctgaaaat tagcctttca
    10921 gacctaaacg ctcgccctga tagctgccgt cctgcacctg cttccaccag ctttcattag
    10981 ccagatacca ctgcaccgtt ttgcgcatcc cactttcaaa ggtttcctgc ggcagccagc
    11041 caagttcatg ggcaattttc gaagcatcaa tggcataacg cagatcatgc cccggacggt
    11101 cagcgacaaa ggtgatcagg tcacgatagt gcgccacgcc gtgcggcttg ttcggagcca
    11161 gttcttccag cagctcgcaa atggtttcca caacatcgag attcttacgc tcgttgtgac
    11221 caccgatatt ataggtttca ccgactttcc cggtggtcgc cacgcaatac agcgcgcggg
    11281 cgtgatcttc aacatacagc cagtcacgga tttgctgccc gttgccatat accggcagcg
    11341 gtttacccgc cagcgcgttg aggatcatca gcgggatcag tttttccgga aagtggtaag
    11401 gaccgtagtt attcgagcag ttggtgataa gcgtcggcag accgtaggtc cgcagccagg
    11461 cgcgcaccag atggtcgctg ctggcttttg acgcggaata agggctgctc ggcgcatacg
    11521 gcgtggtttc ggtgaagaaa tcatccgtcg agtgcaggtc accatatact tcgtcggtgg
    11581 agatatgatg aaaacggaac gctgattttt tatcttccgt cagcgcattc cagtaagccc
    11641 gcgccgcttc aagcaatgta taagtcccga caatgttggt ttcaataaac gctgccgggc
    11701 cgtcaataga acggtcaaca tggctttcgg ctgccagatg catgacacag tctggctgat
    11761 gcgcagtgaa tacgcgcgcc agttctgccc gatcgcagat atcaactttc tcaaaggcaa
    11821 agcgctcgct ttgcgcgacc ggtgccagcg acatcaggtt tccggcgtag gtcagcttat
    11881 cgaccactac caccgcatcg ctcgtttcgt tgatgatata acgcaccagc gccgagccaa
    11941 taaacccggc accacctgtt atcagaatct ttctcatcag cgccagactc ctttggcatc
    12001 gacgacatac tgctgatgga cattgtcgcc attgataact ttaaactgac tatgatcgac
    12061 cagcatcacc agcacgtctg ccgttgccag cgcctcgtca agctgcgcca gagtacaaag
    12121 cccggtcagt tttttcggca actggtggat gttaggctca acaaccagag tttcgccgct
    12181 atgccactgg gcgatcagtt cagcgatttc catcgccggg ctttcgcgca ggtcatcaat
    12241 attcggttta aacgccagac caaagcaggc gattttcagt tcactggcgc gtttatcggt
    12301 agccgccagg caatcagcca ccgccgcttt cacctgatcg ataacccaga acggtttgtg
    12361 atcgttcact tcgcgcgcgg tacggataag ccgcgcctgc tgggggttct gtgccacgat
    12421 aaaccacgga tcaacagcaa tgcagtgacc gcccacgcca gggccaggct gaagaatatt
    12481 aacgcgaggg tgacgattcg ccaggcgaat cagttcccag acgttaatcc cctgatcggc
    12541 acaaatcagc gacaattcat tagcaaaagc gatattcaca tcgcggaagc tgttttcggt
    12601 gagcttacac atttccgccg tccgcgagtt agtgacgaca cactcacctt cgaggaaaat
    12661 tttgtacagt tcgctggcgc gggtcgaaca aaccggcgtc ataccaccaa tcacgcgatc
    12721 gtttttaatc agctcgacca taacctggcc cggtaacacg cgttccgggc agtaagcaat
    12781 gttgacgtcc gcctgctcgc ccacctgctg tgggaaagtg agatccggac gcatctctgc
    12841 taaccattct gccatcttct cggttgaccc caccggcgag gtggattcaa ggatcaccag
    12901 cgcgcctttt ttcagcactg gcgcaatgga gcgagcagcc gattcaacgt aggtcatatc
    12961 tggctcatga tcgcctttaa acggcgtggg tacagcaatc agccaggcat ccgcttcaac
    13021 tggcgtcgtg ctcgctcgta aaaaaccgcc ttctacggca gtttttacta cgctcgccaa
    13081 atcaggctcg acgatatgga tttcgccacg attgatggta tcaaccgcgt gttggttgat
    13141 atcgacacca atcacctgtt tttgccgtga ggcaaacgct gctgctgtag gcagcccgat
    13201 gtaacccagt ccgataacag aaatggtcgc aaaactcata gtgatatccg attatttttt
    13261 aacgcttcca gaatgcgaga gcatgcctga ccatcaccat acgggttatg ggcgcggctc
    13321 atagtttgat attcgttttc gtcttttaaa agacgcgtca cttcctcgac aattcgctgc
    13381 ttatccgtgc ctaccagacg caccgtaccc gccgtcaccg cttccggacg ctcagtggta
    13441 tcgcgcatca ccagcaccgg tttccccagc gaaggcgctt cttcctgaat gccgcctgag
    13501 tcggtcaaaa tcagccaggc gtggttcatc agccagacaa acggtaaata ctcctgggga
    13561 tcgatcagaa tgacattttt cacatgcccc agaatgcgat tgaccggctc tctgacgttc
    13621 gggttgagat gcaccggata gacaatctgg atgtcctggt gcgtggtggc aatgtctgcc
    13681 aacgcgtggc agatttcttc aaaaccacga ccgaaactct cacgcctgtg accggtcacc
    13741 agaatcatct ttttatcggg gtcgataaac gggtaatttg ccgccagttc tgaacgcagc
    13801 gtgtcgctgc tcatcacctg atcacgcacc cataacagtg catcaatgac tgtattacca
    13861 gtaatgaaga ttcggctatc cgcaacgttt tcacgcagca agttttgccg ggaagtttcg
    13921 gttggagaga agtgatacat cgccagatgc ccggtcaatg tacggttagc ctcttccggc
    13981 cacggcgaat agagatcgcc cgtgcgtaaa ccagcctcaa cgtgaccaac aggaatacgc
    14041 tgataaaacg ccgccaggct ggttgccagc gtcgttgttg tgtcgccgtg aaccagcacg
    14101 acgtctggtt tgaactcggc aagaataggt tttagccctt ccagaatccg acaggttatc
    14161 tctgtcaggc cctgtcctgg ctgcattatg ttgagatcgt agtcaggtac aatggaaaag
    14221 agtttcagca cctgatcgag catctcccga tgctgcgcag tgacgcaaac tttagcctca
    14281 aaaaaaggat cttttgccaa cgcatgcacc aacggagcca tcttgatggc ttccgggcgc
    14341 gtaccaaata cagtcagtac tttcacatcg attctcttcg aataagcggc gagcgccttt
    14401 gcgctcgccg cagcagtgtt gctatttcga gcaacggcgg gttaatgcga caccagcccc
    14461 gatcagcccc ccgacaatgc cccacataat catcaggaag gcacgacgtg ggctatcgcg
    14521 ttttaccggt tcttccggcg tacgcaaata gcgataggtc tgaaaacgcg gatccagagt
    14581 tggaccaaca ttcagggtgt ttaacatggc ccgattctga tcatagtcga gatcaaaggc
    14641 cggaccgacg gcctgtaaat tttccagtcg agcctggagc attgaacgcc caagcaggaa
    14701 catttctgaa tcaggtaatt cctcggcagg cacatctgtc gcactgcgcg aaatattatg
    14761 ctgctcagca attttcagcg cctgctcaat gctgttcatc cggcggtcgt agatggcttt
    14821 cgccacctct tcctgacgct tcacctgagc tttcatctgg atggtgcgtg ccgcccatgc
    14881 gcctttcagc tcatcattca gatggctggc tgcacgctgg ctggcaaaag caacatactg
    14941 acgtaacagg ttattagcgt caggcgcggt ttcggcaata agcttcacgc tgtcattgac
    15001 cgcgcgggta aagtctccgg ggataaactg gatgttgtta atcatttcat ccagcaacgc
    15061 cgcatcggct ttgctgttgc ccaccatccg ctgtttgtaa tagtcggttt gcagccagaa
    15121 ctctctgcgg gtatcccacg aggccagttg cataacaaac tctttatagg cttcgtccat
    15181 gaccgatggt tgatcggcag aagccatgtt tgaacggaca tccaggttac gcaaaaattg
    15241 ctgctgcgag taatatcccc ccagcatatt caccgttgga cgatcggtaa tcgccgtcga
    15301 gctccactcc tgacgagcaa aaaaagtata cgccagcgcg attaacgcaa acgccagccc
    15361 catgccaata atccatagct tcccagccca caaggtacga aacaacccac gaatatccag
    15421 ttcattttca gcgtcttcgg ccgctttccc aggcattggt tgtgtcatca catcctcatt
    15481 tatttggtta aattggggct gccaccacga tttctacgca gtctgcgttt tacgcgctta
    15541 ataaagcgag caactttcca ggcacgctta atgcaatatc catagaggaa gaatgctagc
    15601 aaaaagagca ccagcatgac ccactccggg acaaaatgag aatattctgc cagcacgcca
    15661 atggaagcga gcagtgctgc ggcaagggta atcagcacaa acgcctgacg ggaagtaaac
    15721 ccggcacgca tgatcaaatg gtgaatatgc tgacggtcag gagagaatgg gctcatgcct
    15781 ttacgcaggc gacggtacat aatcgccacc atatccatta gcggaatggc gattatccac
    15841 aaagcggtaa ccgggctgat gggatgggtt ttgccctggg tcgtttcgag caggatccag
    15901 ataacggtaa aaccaatcag cgtactgccc gcatcaccca taaagacttt gtagcggcga
    15961 cccaggatac caaggttaag catgatgtat ggcaggatgg cggcgatcat cgcaaagcac
    16021 cagattgcga ggctggtttg cccgtcgaac cacaaaatca taccgattgc tgcaaacgag
    16081 acgcaggaca acccgcccag caagccatca atgccatcaa ccatgttgaa cgcattaatg
    16141 gccgcccaga cggcaaatag cgtcaggaag taaccaaacg gtccgagcac catctcccag
    16201 gagccaaaga tataacccag gctactgaga taaagtttgc cgaacaccat cataacaatg
    16261 ccaacagcgg cctgtatggt ggcacggatt tttacgctga tatcaaaacg gtcatccagc
    16321 gcgccaatga aaacaagcac accggcacaa gcgagataga gagatgcatg cggaatatag
    16381 taatcgacaa ttccgaacgt gaagcaaatc cctgcgtaaa ccgaaatccc cccaacgaga
    16441 ggtatcaatc cctggtgacg tttgcggaag tttggtttat ccactaaacc gacttttttt
    16501 gccaccttac gggcaaaaaa cagaaacagt gtcgtgaata aaaaaatact gatgagatca
    16561 gtactcactg tcagtaaatt cacaatgcat gctctcagag aaaattatta gcagaagtat
    16621 aaccacgaag acctttattc agaagggaaa gtccgttccg aacccaccag tcctgtgcaa
    16681 aaattaacca tttgatatgc aaattgcata acatgccagc aaattccagg aattttccta
    16741 acatcgccag cgcggcatta agattacaga tataaaagca aaacgccacg taaacacgtg
    16801 gcgtttttgg cataagacaa atttatgagc gtttcatcat ttcgaagaaa tcgtcattgg
    16861 tcttggtcat ggccagttta ttaatgagga attccattgc atcgatttcg cccatcgggt
    16921 gaatgatttt gcgcaggatc cacattttct gcagttcttc ctgagtcgtg agcagctctt
    16981 ctttacgggt accggaacgg ttgtagtcga tagccgggaa gacgcgtttt tcagcgatct
    17041 tacgagagag gtgcagttcc atgttgcctg tacctttaaa ctcttcgtaa ataacttcgt
    17101 ccattttaga accggtatcg ataagcgccg tcgcgataat ggtcaggctg ccgccctctt
    17161 ccacgttacg cgccgcaccg aagaagcgtt tcggacgatg aagggcattg gcgtccacac
    17221 caccggtcaa cactttacct gacgccggaa caacagtgtt gtaagcgcgc gccagacgag
    17281 tgatggagtc gagcagaatg ataacgtctt tcttgtgctc aaccaggcgt ttggccttct
    17341 cgatcaccat ttccgcaacc tgaacgtggc gagatgcggg ttcgtcaaag gtagaagcaa
    17401 caacttcacc ttttaccaga cgctgcatct cggttacttc ttccggacgt tcgtcgatca
    17461 gcagaaccat cagcacacaa tccgggtggt tgtaagcaat gctctgagca atgttctgca
    17521 gcagcatagt tttaccggct ttcggcggtg ccacaatcag accacgctga ccacgaccga
    17581 taggtgatgc cagatccagt acgcgagcgg ttaaatcttc cgtagaaccg ttaccacgtt
    17641 ccatacgcag acgagagttt gcgtgcagcg gggttaagtt ctcaaagagg attttgttgc
    17701 gggcgttttc aggtttgtcg aagttaactt cgttaacttt cagcagcgca aaatagcgtt
    17761 caccttcttt cggcgggcga atcttaccag agatggtatc accagtgcgg aggttgaaac
    17821 ggcggatttg gctaggggaa acgtagatgt catcaggacc ggcgaggtag gagctgtctg
    17881 cggaacggag gaaaccaaat ccatcctgca atatctccag tacgccatca ccaaagatat
    17941 cttcgccact ctttgcgtgc tgcttcagga tggcaaaaat aatgtcctgc ttacgcatac
    18001 gagccaggtt ttccagcccc atattttcgc cgagagtgat cagctcagaa accggcgtat
    18061 tctttaattc ggtaagattc atagtggtgt gagttcttaa acttggggta aatctcgaac
    18121 ttaatgttgt gaatggtatg gcaggatcat ccatgcctgt ttacggtcat cgactcatgt
    18181 cttttcgctg cctggtcaca ggagagtacg cagaactgaa acgacaagac ggattgagtg
    18241 acgagccaga aatctgttca cttcgcattt aagataaaac gggaagcgtt ggaaattaca
    18301 agattcaaac ttaataaggt atgtttaata cgaagtcaac actaagttag catgactcac
    18361 gccgggcgtc cagtttttag cgacggggca cccgaacatg aaattccctt acgccaggtt
    18421 agcgtcgagg aactctttca actgaccttt agacagtgca cccactttgg ttgccgccac
    18481 ttcaccgttt ttgaacagca gcagagtcgg gataccacgg atgccatatt tcggcgcagt
    18541 gccagggttt tgatcgatgt tcagttttgc aacggtcagt ttgccctgat attcgtcagc
    18601 gatttcatcc agaatcgggg cgatcatttt gcacggaccg caccactctg cccagaaatc
    18661 gacgaggatc gccccgtccg ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg
    18721 aataatttta tcgctcatat ataactccac aggaataagc ctggcgtgtt ggtttcgttg
    18781 ttggtgtaac attaaccaac taaaggttga ctttatttca ccggatacgc tttcgtaaag
    18841 caatagtaag ctgatattct accacactat gagcaaaaca catttaacag aacagaagtt
    18901 ttccgacttc gccctgcatc cgaaggttgt agaagccctt gaaaaaaaag ggtttcataa
    18961 ctgtacgccc attcaggcac tggcccttcc gctgacgctg gcgggtcgtg acgtagccgg
    19021 gcaggcgcaa accggtaccg ggaaaacgat ggcgtttctt acgtcaacgt ttcattatct
    19081 tctctctcat cctgcgattg ccgatcgcaa ggtgaatcaa ccacgtgcct taattatggc
    19141 accgacgcgt gaacttgccg tgcagatcca tgccgacgca gaaccgctgg cggaagctac
    19201 cggcctgaag ctgggtctgg cttacggtgg tgatggctac gacaaacagc tgaaagtgct
    19261 ggaaagcggc gttgacattc tgattggcac tacggggcgt ttaattgact acgccaagca
    19321 gaaccacatt aacctcggtg ccattcaggt ggtggtactg gacgaagccg atcgcatgta
    19381 cgatctgggc tttattaaag atatccgctg gctgttccgc cgtatgccgc ctgcaaacca
    19441 gcgcctcaac atgctgttct ccgccacgct ttcgtaccgg gtacgtgaac tggcgttcga
    19501 gcagatgaac aatgccgaat atattgaagt ggaaccggaa cagaaaacgg gccaccgtat
    19561 taaagaagag cttttctacc cttctaacga agaaaaaatg cgtttgctgc aaacgctgat
    19621 cgaagaagag tggccagaca gagcgattat tttcgccaat accaaacacc gttgtgaaga
    19681 gatctggggc cacctggcgg cagatggtca tcgtgtcggt ttgttgacag gcgatgtcgc
    19741 gcagaaaaaa cgtctgcgta ttcttgatga atttacccgt ggcgatctgg atattctggt
    19801 tgccaccgac gttgccgcgc gtggtttgca tattccggca gtgacgcacg tctttaacta
    19861 cgatttaccc gatgactgtg aagattacgt tcaccgtatt ggtcgtacag gtcgcgcagg
    19921 cgcaagcggt cactctatca gcctggcgtg tgaagagtat gcattgaatt tgcctgctat
    19981 tgagacctat attggtcact caattccggt aagcaaatac aatccggacg cattgatgac
    20041 cgatctgcca aaaccgctgc gcctcacgcg cccacgtaca ggcaatggtc cgcgtcgtac
    20101 tggcgctccg cgtaatcgtc gtcgttcagg ttaaataaaa tgtcatactc aaaatcattc
    20161 aaactgtatc aagatggcaa gagaatgaat cccgatgagc atactgtagt aagtgattca
    20221 ggtgaaagaa tgcagccaac acagagacag attgaaggat gaagagtatg ggttccacct
    20281 cgtcgctgta tgcagccatt gatctcggtt cgaatagttt tcatatgctg gttgtgcgcg
    20341 aggtggctgg aagcatccag acgctgacgc gaataaaacg caaagtgcgt ctggctgctg
    20401 gcctgaatag cgaaaatgcc ctgtctaatg aagcaatgga gcgcggttgg caatgtctgc
    20461 gcctgtttgc tgaacgtctg caagatatcc ctccctcgca aattcgcgtt gtcgctacgg
    20521 cgacattacg tcttgccgtc aatgcgggtg attttattgc caaagcgcag gaaatcctcg
    20581 gttgtccggt acaggtgatc agcggtgaag aggaagcacg tctgatttat cagggcgttg
    20641 ctcacacaac tggcggtgcc gatcagcgcc tggtggtgga tataggcggt gccagtactg
    20701 aactggtaac cggcacgggt gcacaaacca cctcgttgtt cagcctgtcg atgggctgcg
    20761 tcacctggct ggaacgctat tttgccgatc gtaatctggg gcaggaaaat tttgatgctg
    20821 cagaaaaagc ggcacgcgaa gtgttacgtc cggttgccga tgaattacgg tatcacggct
    20881 ggaaagtgtg cgttggtgct tccggcaccg tgcaggcgtt acaggaaatc atgatggcac
    20941 aggggatgga tgaacgcatt accctggaaa agttgcagca attgaaacag cgagccattc
    21001 attgcggtcg gctggaggaa ctggagattg acgggctgac gctggaacgt gcgttagtgt
    21061 tcccgagtgg tctggcgatc ctgatcgcca tttttaccga actgaatatt cagtgtatga
    21121 ccctggcggg cggtgcgctg cgtgaaggcc tggtctacgg catgttgcat ctggccgtcg
    21181 agcaggatat tcgcagccgt acgctgcgta atattcagcg ccgctttatg atcgatattg
    21241 atcaggcgca gcgcgtagcc aaagttgcgg ctaacttctt cgatcaggtg gaaaaggaat
    21301 ggcatcttga agcaataagc cgcgatttgc tcatcagcgc ctgtcagctt catgaaatcg
    21361 gcctgagcgt tgactttaaa caagcgccgc aacacgctgc ttatctggtg cgtaatctgg
    21421 atcttcccgg ttttaccccc gcacagaaaa aattgctggc gacgctactg ctcaaccaga
    21481 ctaatccggt cgatctctca tcgctgcatc agcaaaatgc cgtaccgccg cgcgtcgcag
    21541 aacaactctg ccgtttactc cgcctggcca tcatttttgc cagccgtcgc cgtgacgatc
    21601 tcgtgccaga gatgacatta caggctaacc atgaactgtt gaccttgacg cttccgcaag
    21661 gttggctaac ccaacatccg ctgggtaaag agattattgc tcaggaaagc cagtggcaga
    21721 gctatgtcca ctggccgctg gaagtgcatt aatgagtaag tgccggatgc gatgctgacg
    21781 catcttttcc ggccttgatt atttccctcg ttttgccgcc atcatcgctt tcagattcgc
    21841 cagatggctt tgccctttct gcatccgttc ttcggcgctg accactttgc gctcctgttc
    21901 ccaaatcaga tcatcctgcg gcagctccag caaaaagcgg ctcggctccg ggcgcaccag
    21961 ctcgccgtac tgacggcgtt ctttacacag cgtaaaggtc aattccttct gggcgcgggt
    22021 aatgccgaca taggccagcc gccgctcctc atcgatatta tcttcatcga tgctgctctg
    22081 gtgcggcaaa aacccttctt ccataccgac catgtagaca taaggaaact ccagcccttt
    22141 tgacgcgtgc agagtcatca gttgcacctg atccagctct tcttcactct caccacgctc
    22201 catcatgtcg cgcaaagtaa agcgcgtcac cacctgggtg agcgtcatcg gctcatccag
    22261 ttcgctgcct tccagcatct ccgtcatcca gctaaacagt tggttgacgt tcttcatgcg
    22321 catttcggcg gctttcgggc tgggcgatgt ttcgtacagc caggattcat aatccatgcc
    22381 gtggatcaga tcgcgcaccg cggcaatcgg ctcccgctcc gccagacgct ggatttctgc
    22441 caaccagtga gtaaagcggg tcaatgcttc ataaccacgt ccgctaagcg tctgactcag
    22501 gcccatatca aagctggcgg taaacatgct tttattgcgc gtcatcgccc actcacccag
    22561 ctttttcagc gtcgccgggc caatctctct cttcggcgtg ttaacgatac gcagaaatgc
    22621 gctgtcatcg tccgggttag tcagcacgcg cagataagcc agcaagtcct tgatttcagg
    22681 acgagagaaa aacgacgtac caccagaaat tttgtacggg atgcggtttt gcatcaggaa
    22741 tttttcaaac actcgcgact gatggttacc gcgataaaga atagcgtaat ctttgtactg
    22801 cgttttattg acgaagtgat gcgcaatcag ttcaccagtg acgcgctcgg cttcatgttc
    22861 ttcgttattc gcgcttaaaa ctttaagctc tgtgccgtaa cccagttcgg agaacagacg
    22921 cttctcaaag acgtgcgggt tattggcgat caggatgttc gccgctttca aaatacgtcc
    22981 ggaagagcgg tagttctgct caagcttaat caccttcagc gccggaaaat cctgactcag
    23041 cagcaccagg ttttgtggac gtgcgccgcg ccaggagtag atcgactggt cgtcatcgcc
    23101 caccacggta aagcgcgcgc ggctgcccac cagcagtttc accagttcat actggctggt
    23161 gttggtgtcc tgatactcat ccaccagcag atagcgaatt ttgttctgcc agcgctcgcg
    23221 gacttcttca ttgcgttgca gcagcaacgt cggcaataaa atcagatcat cgaagtcgag
    23281 aacattgcac gctttcaggt gtgcatcata cagcccataa caatgggcaa aaatacgatc
    23341 ccgctcgccc tttgcctctg ccgccgcctg cgccggagtt ttgagatcgt tcttccagtt
    23401 ggagatggtc gaaatcagct gttgcaggag aactttgtca tcttcaatca gcccctcggt
    23461 cagctcttta agcaaagcaa gctgatcggt atcgtcaaac aacgagaagt tcgctttcat
    23521 cccaagcgcc gcatactcgc gtttgatgat atccaacccc agcgtgtgaa aagtggagat
    23581 catcagccca cgcgcctctt tgcgacccag cgtctgcccg acacgctctt tcatctcgcg
    23641 cgccgcctta ttggtaaagg tcaccgccgc gatatgtcgc gcctgataac cgcaaccgcg
    23701 gatcagatgg gcgattttat tggtgatcac acgagtttta ccggaacccg cgcccgccag
    23761 caccaggcag gggccggtaa cgaattcgac agcttgttgt tggccggggt ttagacgcat
    23821 aggtgtattg ctcaatcttc gaacgggggg aggattgtag cagaatcgca tttgaatgtg
    23881 accgcgtgta agccgctaac cggttggctg cggatgttta aatcagagag ggaagaccga
    23941 aaccggagtt catcgtgaat ccattagact tagaaaatat cgggtcgtaa cgttgtgtta
    24001 ttactttttc agagtttact aaggagaggt gtactcgtgg cgattaattt cagtcctaag
    24061 gttggtgaaa tactggaatg caactttggt aactatcctg ttagccagaa tggacagttc
    24121 agcaccacgt attacgatgg ccgcattcct cctgaaatga taaaaaacag gttagttgtc
    24181 gtgctcaacg gaaaaattaa tggcaacgca tgtatcgtcg ttccattatc aaccactcgc
    24241 gatcatgaca agttaaatcg tggaatgcat gttgaaatag ccagtaatgt tatcaatgac
    24301 ctgcaattct ttgatcagca gattcgttgg gccaaagccg acctggtaca acaagtcagt
    24361 cgaaaccgat taaacagagc aaggacttac agagggtatc ttaatcaatg tttgccacat
    24421 gagttggttg cagacataca gagagcggtc atcaagtcaa tcaatgccat atcgctcata
    24481 aattgacaat aaaatcacca tccccgataa tgactctgta gcccatagag gccctaagtt
    24541 ttctcccctt gagggacctg cgaagcccgg attttccggg cttttgccat atatgtacca
    24601 gccccttcct gactgacaat tacgttagca tctgcggatc atgctaaaat cgccgcctga
    24661 caattattca ctcaaggcac gatcatggca aaaacagcag cagcactgca tatccttgta
    24721 aaagaagaga aactggctct ggatcttctc gagcagatta agaacggtgc cgatttcggc
    24781 aagctggcga agaaacactc catttgccca tcaggcaaac gcggcggtga tttaggtgaa
    24841 ttccgccagg gtcagatggt tccggcgttc gataaagttg tgttctcttg tccggtactg
    24901 gagccaaccg gcccgctgca cacccagttc ggttaccaca tcattaaggt gctgtaccgt
    24961 aactaatagc aaggccttct ccaggagaag gccttgagtg ttttctccct ctccctgtgg
    25021 gagagggtcg gggtgagggc atcagcgcgc acttaacccg caactgcaat acgtttcata
    25081 tccgtcatat agccgcgcag tttcttacct acctgctcaa tcgcatggca gcgaatcgct
    25141 tcgttcacat cacgcagttg cgcgttatct accgcacctt ccggaatagc tttacccaga
    25201 tcgcccggtt gcagctctgc cataaacggt ttcagcagcg gcacacaagc gtaagagaac
    25261 agatagttac cgtactcggc ggtatcagag ataaccacgt tcatttcgta cagacgctta
    25321 cgggcgatgg tgttggcaat cagcggcagt tcgtgcagtg attcatagta agcagattct
    25381 tcgatgatgc cggaatcaac catggtttca aacgccagct caacgcccgc tttcaccatc
    25441 gcgatcatca gtacgccttt atcgaagtac tcctgctcgc cgattttgcc ttcatactgc
    25501 ggcgcggttt cgaaggcggt tttgccggtc tcttcacgcc aggtcagcag tttcttatca
    25561 tcgttggccc agtccgccat cataccggag gagaattcgc cggagatgat gtcgtccata
    25621 tgtttctgga acaggggtgc catgatctct ttcagctgtt cagacagcgc gtaagcacgc
    25681 agtttcgccg ggttagagag acggtccatc atcagggtga tgccgccctg cttcagcgct
    25741 tcggtgatgg tttcccaacc gaactgaatt agtttttctg cgtatgccgg atcggtgccc
    25801 tcttccacca gcttgtcgaa gcacagcagc gagccagcct gcaacatacc gcacaggatg
    25861 gtttgctcac ccatcaggtc agatttcact tccgcaacga aggacgattc cagcacgccc
    25921 gcacggtgac caccggttgc tgccgcccat gctttagcga tcgccatacc ttcgcctttc
    25981 ggatcgtttt ccgggtgaac ggcaatcagc gtcggtacgc cgaatccacg tttgtactct
    26041 tcacgcactt cggtgccagg gcatttcggc gcaaccatga ctacggtgat atctttacgg
    26101 atctgctcgc ccacttcgac gatgttgaaa ccgtgcgagt aacccagcgc cgcgccgtct
    26161 ttcatcagtg gctgtacggt gcgcactaca tcagagtgct gcttgtccgg cgtcaggtta
    26221 accaccagat ccgcctgcgg gatcagttct tcgtaagtac caactttaaa accgttttcg
    26281 gtcgctttac gccaggacgc gcgcttctcg gcaatcgctt ctttacgcag agcgtaggag
    26341 atatcgagac cagaatcacg catgttcagg ccctggttca gcccctgtgc gccacagccg
    26401 acgataacca cttttttacc ctgaaggtag ctcgcgccgt cggcgaattc gtcgcgcccc
    26461 ataaagcgac atttgcccag ctgtgccagc tgctggcgca ggttcagtgt attgaagtag
    26521 ttagccatgg tgattcctcg tgatgttgtg cttcttattg ttcggttcgc gtttgcgaga
    26581 ttgaattcac tatatgacag gaaatttatt gcggaaattg atatattcac aacgtcacat
    26641 tgcaatttct gcaacgtcaa cattgagggc tgcgccattg gatttacgcg atctgaaaac
    26701 cttcctgcat ctggcggaaa gccgccattt tggccgcagc gcgcgggcga tgcacgttag
    26761 cccgtccacg ctctcacggc agattcagcg cctggaagac gatctcggtc aggcattatt
    26821 tgtgcgcgat aaccggacgg tgacgctgac cgaagcgggc gaagagttac gcgttttcgc
    26881 ccagcaaaca ttgttgcagt atcagcaact gcgccacacc atcgatcagc aagggccgtc
    26941 gctctctggc gagttacata ttttctgctc ggtgaccgcc gcttacagcc atctaccgcc
    27001 gattctcgac cgcttccgcg cggaacaccc gtcggtggag attaaactta ctactggtga
    27061 tgcggcagat gcgatggaaa aggtggtcac tggtgaagcg gatctggcga ttgcgggtaa
    27121 accggaaacc ttgcccggcg cagtggcgtt ttcgatgctg gagaatctgg cagtagtgct
    27181 gattgccccc gcgctgccct gcccggtgcg taatcaggtg tcggtagaga agccggactg
    27241 gtcaacggtg ccgtttatca tggccgatca ggggccggta cgccgccgca ttgaactgtg
    27301 gtttcggcgt aacaaaatca gtaacccgat gatttacgcc acggttggcg ggcatgaagc
    27361 gatggtatcg atggtggcgc tcggctgtgg cgtggcgctg ttgccggaag tagtgctgga
    27421 aaacagcccc gaaccggtgc gtaaccgcgt gatgatttta gagcgcagcg atgaaaaaac
    27481 gccgtttgaa ctgggcgtgt gtgcacaaaa aaagcggctg catgagccgc tgattgaggc
    27541 gttctggagg attttgccga accacaaatg acgttgtcgc gcgggtaggc ctgataagcg
    27601 aagcgctatc aggcattttt ccctaacccg ccaaaaagaa cctgaacgcc gggttattgg
    27661 tttcgtcgtg gcaatcgtag cccagctcat tcagccgggt ttcgaaatcc ggttcatggt
    27721 cgccaagttc gaacgccgcc agtacgcgcc cgtagtcggt gccatggctg cgatagtgga
    27781 acaaagaaat gttccagtgc gtacccagcg tgttgaggaa gcgcagcagc gcgcccggtg
    27841 attccgggaa ttcgaagctg tagaggcgtt cctgcaacgg atgtgacgga cgcccgccga
    27901 ccatatagcg cacatgcaac ttcgccattt cgtcgtcgga gagatccacc acgctgtagc
    27961 cgccgtcgtt gagcatctgc aaaatttctt tgcgctcttc gaggccgcgg ctcaggcgca
    28021 cgccgacaaa gatgcaggcg tttttggcat cggcaaaacg gtagttgaac tcggtgaccg
    28081 atcgcccgcc aagcagttgg cagaatttga ggaagctgcc tttttcttcc ggaatggtca
    28141 ccgccaacaa cgcttcacgc tgttcgccca gttcgcagcg ttccgagacg tagcgcaggc
    28201 cgtggaagtt cacgttggca ccggaaagaa tatgcgccag ccgttcgccg cgaatgttgt
    28261 gctgggcgat atattttttc attcccgcca gcgccagcgc gccagagggt tccgccaccg
    28321 cacgcacatc ttcgaataaa tccttcatcg ccgcacagat cgcatcgcta tcgacggtga
    28381 tgatgtcgtc gagatactcc tggcataaac ggaaggtttc gtcaccgatg cgttttaccg
    28441 cgacaccttc ggcgaacagt cccacgcgtg gcagatcaac cggatgaccc gcatccagcg
    28501 ctgctttcag gcaggcggag tcttccgctt ctacggcgat cactttgatt tgcggcatca
    28561 gttgtttgat cagcaccgct acgcccgccg ccagaccgcc gccgccgact ggcacaaata
    28621 cgcggtcgag atgggcgtcc tgctggagca gttccagcgc cagcgtgcct tgcccggcaa
    28681 tcaccatcgg atggtcgaac ggcggcaccc aggtgaaccc ctgctgctgt gacagttcga
    28741 tcgctttagc tttcgcttcg tcaaagttcg cgccatgaag cagcacttcg ccgccgaatc
    28801 cacgtaccgc atcgactttg atgtcggcgg tggcggttgg cataacgatc agggccttca
    28861 cgcctaaccg cgcagaagaa aacgcgacgc cctgcgcgtg gttacccgca gaagcagtga
    28921 tcacgccgtg cgctttctgt tcttccgtca ggcccgccat catggcgtat gcgccgcgca
    28981 gcttaaagct atgaactggc tgacgatctt cgcgcttcac cagaatgacg ttatcaagac
    29041 gagacgagag cttttccatt ttttgtagcg gcgtgacctg cgccgcttcg tagaccggcg
    29101 cgcgcagcac cgctcttaaa tattcggcac cttccggagc accggacagg ggttgtgagt
    29161 cagccatcat taacccccca gtttcgattt atcgcgcacc gcacctttgt cggcgctggt
    29221 cgccaggctg gcgtaggcac gcagggcaaa ggaaacctga cgttcacggt ttttcggcgt
    29281 ccaggctttg tcaccccgag cttcctgcgc ttcacgacgc gccgccagtt cggcatcgct
    29341 tacctgtaac tgaatgccac ggttcgggat gtcgatagcg atcaggtcac catcttcaat
    29401 caggccaatg ctgccgccgc ttgccgcttc cggtgagacg tggccgatgg aaagaccaga
    29461 ggtgccgcca gagaaacgac cgtcggtgat cagcgcacag gctttaccga gacccattga
    29521 tttcaggaag ctggttgggt agagcatttc ctgcatgcca gggccgcctt tcgggccttc
    29581 atagcggatc accaccacat cgcccgcgac aactttgcca ccgaggatcg cttctaccgc
    29641 gtcgtcctgg ctttcgtaca ctttcgccgg gccggtgaat ttgaggatgc tgtcatcgac
    29701 gcccgcggtt ttaacgatgc agccgttttc tgcgaaatta ccgtagagca ccgccaggcc
    29761 gccgtctttg ctgtaggcgt gttccagcga gcggatacag ccattggcgc gatcgtcatc
    29821 gagagaatcc caacggcaat cctgcgagaa tgcctgtgtg gtacgaatgc ctgcaggacc
    29881 tgcgcggaac atatttttta ccgcgtcatc ctgggtcagc ataacgtcgt attgttccag
    29941 cgtttgcggc aacgtcaggc caagtacgtt tttcacatca cggttcagta accccgcgcg
    30001 atccagttcg ccgagaatac cgataacacc accagcacgg tgaacatctt ccatatggta
    30061 tttctgggtg ctcggcgcaa ctttacacag ctgtggaacc ttgcgggaaa gcttatcgat
    30121 atcactcatg gtgaagtcga tttccgcttc ctgcgccgcc gccagcaggt gaagtacggt
    30181 gttagtcgat ccacccatcg cgatatccag cgtcatggcg ttttcaaacg ccgccttact
    30241 ggcgatatta cgcggcagtg cactttcgtc gttttgctcg taataacgtt tggtcaattc
    30301 aacaatgcgt ttaccagcat taaggaacag ctgcttacgg tcggcgtggg ttgccagcag
    30361 cgagccgttg cccggctgcg acaggcccag cgcttcggtc aggcagttca ttgagttagc
    30421 ggtaaacatc ccggagcagg aaccgcaggt cggacacgcg gaacgttcaa cctgatcgct
    30481 ctgggagtca gatactttcg ggtctgcgcc ctggatcatc gcatcaacca gatcgagctt
    30541 gatgatctga tcggaaagtt tggttttccc ggcctccatc gggccgccgg aaacaaagat
    30601 caccggaata ttcaggcgca gggaagccat cagcatcccc ggggtgattt tgtcgcagtt
    30661 agagatgcag accatggcgt cggcgcagtg ggcgttgacc atatactcaa cggaatcagc
    30721 gatcagttcg cgagatggca gtgaataaag catccccccg tggcccatgg caatcccatc
    30781 atccaccgca atggtgttga actctttggc aacgccgcca gccgcttcaa tttgttcggc
    30841 gaccagttta ccgagatcgc gcagatggac gtgacccggt acaaattggg tgaacgagtt
    30901 cacaaccgcg ataatcggct taccgaaatc ggcgtcggtc attccggtgg cgcgccacag
    30961 cgcacgagca cccgccatat tacgaccatg agtggtggtg gcggaacggt acttaggcat
    31021 actttattta ctcccagtgt ctgtctcgta aatgggacgg tgcgtgccgt cccatttttt
    31081 gtatttattg attaacttga tctaaccagc cccatttatc ttcggtttcg ccagtgaaga
    31141 ggccgaagaa ggcttgctga atgcgtttgg taaccgggcc acaacggcct tcgccaacct
    31201 gaataccgtc tacgctgcgc actggcgtga tttctgccgc cgtaccggac ataaacactt
    31261 catccgccag gtacagggat tcgcgcgaca gcacctgctc acgtacttca attcccagct
    31321 ctttcgccag tttgatgatg gcatcacggg taatacccgg cagcgcggag gaggtgaacg
    31381 gtggggtgaa cagcacacca tctttcactt caaacaggtt ttcgcctgcg ccttcagaga
    31441 tataaccgtt cacatccagc gcgatacctt cctgataacc gtggcggcgc gcttcgctac
    31501 ccaccagcag ggaagagaga tagttaccac cggcttttgc cgcagttggg atggtgtttg
    31561 gtgctgcgcg gttccaagag gaaaccatcg catcgatccc ctgctccagc gcttctgcgc
    31621 ccagatacgc tccccacggg aaagcggcga taatcacatc ggttgagtat cccgctggcg
    31681 ggtttacacc catgccaaca tcaccaacga agatcagcgg acggatatag gcgctggtga
    31741 gattattttt gcggatcacg tcacgacaag cttccatcag cccatcaatg ctctgcgaaa
    31801 ccgggaagcg atagattttg gcggagtcat gcagacgctg catatgctca cgatggcgga
    31861 atacaaccgg tcctttgtgc gagtcgtagc aacggatgcc ttcaaaaacc gaagtgccat
    31921 agtgcagcgc gtgcgacatc acatgcacct tcgcgtcttc ccagcgaacc atctccccat
    31981 tgaaccaaat gtaatcagct ttcttcgtgg tcatttttat attccttttg cgctcaggcg
    32041 cggatttgtt gtgatgtggt tgtgctctgg cagatggcaa cgtgtgcgac gtccaccagt
    32101 ttatttaact gactaaacag taagtcgacc gaccgtgggc tggcaacggt caattcgata
    32161 tttatatttt gtgcatcgct ggcggcggcc atattcattg agcagacgtg gaaaccacga
    32221 tgacgcacca cgcgtaaaac acgttctaag gtttctggat tgaagcgagc cgatacattg
    32281 acctgatgtt gcatcatgat aatttctcca atatttctga attactggcg ccaggcggca
    32341 ccagcggcca gacgttctca agttcgtcga ttgagacatg aagcaggtat ggcccatcac
    32401 tgttcagcat ggtgtcgagt gccgcttcaa cctggtcttt acgggtgatg tgttggccag
    32461 ggatgccgaa ggcgctggct aacatgagga aatcggggtt atcagtaagg gtggtttcgc
    32521 tgtatcgttc ctgaaaaaac agttgctgcc attgtcgaac catccctaac cgttggttat
    32581 cgagtaagac gattttcaac ggtaactgct tgcgttttac ggtgcccagc tcttgcacat
    32641 tcatcatgaa agagccgtca ccggagatac agacaacggt atcgttcggt cgcgcgactt
    32701 gtgcgccaac cgccgccggt aaaccaaaac ccatggtacc taagccgctg gaggtgatga
    32761 aattttccgg gcgagtgtgg gcgatgtgct gcgcagccca catctggtgc tgccccacat
    32821 ctgtggtcac gacgcaatcc gcaggtttac gatccgacag ttgttttaac aacaacggcg
    32881 cgtagatagc gtcaccggga tggtcgtaac gccaggaatg ttcatcacgc agctgcgcgc
    32941 agtgttgctg ccagtcattg atatttaacg gctgctgtaa tgctggtaac agagcattta
    33001 aatcaccttg taatgccaca tgtgcctgac gcagcttgtt catttctgcc gggtcgatat
    33061 ccatatggat aacactggcg tgtggcgcga aggtgttcag tttgccggtc acccggtcat
    33121 caaaacgtgc acccacggcg atcagcaagt cgcactcctg caccgcgaag ttcgccgctt
    33181 tggtgccgtg cattcccagc atgcccagat agtacggata atctgcttca actgcgccca
    33241 gccctttcag cgtgcaggtg gcaggcattt ttgtggtagc gagaaattct cgtaaagcag
    33301 gaactgcctg cgccataccc acgccaccac caacgtacag catcggtttt tgcgcttttg
    33361 ccagcatctg gcgcgcttgc tcaacttcgg catgggggaa agtcacttcg ttttcaacgg
    33421 tggtgaacca cggttccaga tcgccgctgg ctaactggat atcttttggg atatcgacca
    33481 gaaccggacc aggacgacct gagctggcaa cgtcgaatgc ttcagccatg atgcgcggca
    33541 actcttccag cgactgcacc agaaagctgt gcttggtaca ggctaacgac aatcccagga
    33601 catccacttc ctgaaatgcg tcggtgccga taaacggtgc ggacacctga ccagtgatgg
    33661 caacgacagg gatggaatct aacagtgcgt ccgcaagccc ggttatcagg ttggttgcgc
    33721 ccggaccaga cgtggcgata catacgccag ttttaccggt agcacgggca taaccgatag
    33781 ccgccattgc cgcaccctgc tcatgtcggc acagcaagtg ctccacgccg ccgtcataca
    33841 atgcatcgta aaccggcata attgcgccac ccggataacc gaaaacggtg ttcacaccct
    33901 gtgcccgcaa cgcatgtacc acccactgtg cgccattcat agttagttcc ccgtcctgaa
    33961 tcttgagaaa cagaattttg tgctgttatt cattgtctgc tcctcggtta tgtttttaag
    34021 gtcaaaaaaa acccccggac ctttcggtgc gggggtctta gttcgttaag gcttgatttc
    34081 taagcctttc ctcgtccaag tgcagccccg cacggtggga taataatcac caccacgcta
    34141 atcacgacca ggctaatcac tcgtagaagg gctgtcattt tgtcttttct tgcatcttgt
    34201 tcgaaggaat gcctaaagag ttaccat
//
LOCUS       DAZKHC020000045        34005 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZKHC020000045 DAZKHC020000000
VERSION     DAZKHC020000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.045"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..2141)
                     /gene="tynA"
                     /locus_tag="ACHOSM_RS12365"
                     /old_locus_tag="pgaptmp_002473"
                     /pseudo
     CDS             complement(1..2141)
                     /gene="tynA"
                     /locus_tag="ACHOSM_RS12365"
                     /old_locus_tag="pgaptmp_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
     gene            complement(2389..4434)
                     /gene="paaZ"
                     /locus_tag="ACHOSM_RS12370"
                     /old_locus_tag="pgaptmp_002474"
     CDS             complement(2389..4434)
                     /gene="paaZ"
                     /locus_tag="ACHOSM_RS12370"
                     /old_locus_tag="pgaptmp_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HGU4558971.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVSSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLLHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            4719..5648
                     /gene="paaA"
                     /locus_tag="ACHOSM_RS12375"
                     /old_locus_tag="pgaptmp_002475"
     CDS             4719..5648
                     /gene="paaA"
                     /locus_tag="ACHOSM_RS12375"
                     /old_locus_tag="pgaptmp_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HGU4558972.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWNTRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            5660..5947
                     /gene="paaB"
                     /locus_tag="ACHOSM_RS12380"
                     /old_locus_tag="pgaptmp_002476"
     CDS             5660..5947
                     /gene="paaB"
                     /locus_tag="ACHOSM_RS12380"
                     /old_locus_tag="pgaptmp_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HGU4558973.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            5956..6702
                     /gene="paaC"
                     /locus_tag="ACHOSM_RS12385"
                     /old_locus_tag="pgaptmp_002477"
     CDS             5956..6702
                     /gene="paaC"
                     /locus_tag="ACHOSM_RS12385"
                     /old_locus_tag="pgaptmp_002477"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HGU4558974.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESREPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIVVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            6717..7214
                     /gene="paaD"
                     /locus_tag="ACHOSM_RS12390"
                     /old_locus_tag="pgaptmp_002478"
     CDS             6717..7214
                     /gene="paaD"
                     /locus_tag="ACHOSM_RS12390"
                     /old_locus_tag="pgaptmp_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HGU4558975.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMSTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLREYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            7222..8292
                     /gene="paaE"
                     /locus_tag="ACHOSM_RS12395"
                     /old_locus_tag="pgaptmp_002479"
     CDS             7222..8292
                     /gene="paaE"
                     /locus_tag="ACHOSM_RS12395"
                     /old_locus_tag="pgaptmp_002479"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HGU4558976.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDDAETALKALGMPEKAIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            8289..9056
                     /gene="paaF"
                     /locus_tag="ACHOSM_RS12400"
                     /old_locus_tag="pgaptmp_002480"
     CDS             8289..9056
                     /gene="paaF"
                     /locus_tag="ACHOSM_RS12400"
                     /old_locus_tag="pgaptmp_002480"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HGU4558977.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLTQLVNELEAAAID
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            9056..9844
                     /gene="paaG"
                     /locus_tag="ACHOSM_RS12405"
                     /old_locus_tag="pgaptmp_002481"
     CDS             9056..9844
                     /gene="paaG"
                     /locus_tag="ACHOSM_RS12405"
                     /old_locus_tag="pgaptmp_002481"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HGU4558978.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            9846..11273
                     /gene="paaH"
                     /locus_tag="ACHOSM_RS12410"
                     /old_locus_tag="pgaptmp_002482"
     CDS             9846..11273
                     /gene="paaH"
                     /locus_tag="ACHOSM_RS12410"
                     /old_locus_tag="pgaptmp_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HGU4558979.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPATVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            11263..11685
                     /gene="paaI"
                     /locus_tag="ACHOSM_RS12415"
                     /old_locus_tag="pgaptmp_002483"
     CDS             11263..11685
                     /gene="paaI"
                     /locus_tag="ACHOSM_RS12415"
                     /old_locus_tag="pgaptmp_002483"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HGU4558980.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRLGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            11685..12890
                     /gene="paaJ"
                     /locus_tag="ACHOSM_RS12420"
                     /old_locus_tag="pgaptmp_002484"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             11685..12890
                     /gene="paaJ"
                     /locus_tag="ACHOSM_RS12420"
                     /old_locus_tag="pgaptmp_002484"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HGU4558981.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGVAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKAASAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQAVGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            12917..14230
                     /gene="paaK"
                     /locus_tag="ACHOSM_RS12425"
                     /old_locus_tag="pgaptmp_002485"
     CDS             12917..14230
                     /gene="paaK"
                     /locus_tag="ACHOSM_RS12425"
                     /old_locus_tag="pgaptmp_002485"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HGU4558982.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGDTVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            14331..15281
                     /gene="paaX"
                     /locus_tag="ACHOSM_RS12430"
                     /old_locus_tag="pgaptmp_002486"
     CDS             14331..15281
                     /gene="paaX"
                     /locus_tag="ACHOSM_RS12430"
                     /old_locus_tag="pgaptmp_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HGU4558983.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIQQEALCQFIR"
     gene            15263..15853
                     /gene="paaY"
                     /locus_tag="ACHOSM_RS12435"
                     /old_locus_tag="pgaptmp_002487"
     CDS             15263..15853
                     /gene="paaY"
                     /locus_tag="ACHOSM_RS12435"
                     /old_locus_tag="pgaptmp_002487"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HGU4558984.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     LMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            16085..16945
                     /gene="pdxI"
                     /locus_tag="ACHOSM_RS12440"
                     /old_locus_tag="pgaptmp_002488"
     CDS             16085..16945
                     /gene="pdxI"
                     /locus_tag="ACHOSM_RS12440"
                     /old_locus_tag="pgaptmp_002488"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HGU4558985.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVTHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            17009..19315
                     /gene="ydbD"
                     /locus_tag="ACHOSM_RS12445"
                     /old_locus_tag="pgaptmp_002489"
     CDS             17009..19315
                     /gene="ydbD"
                     /locus_tag="ACHOSM_RS12445"
                     /old_locus_tag="pgaptmp_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HGU4558986.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVITLAKRDWDMIIQELYKDAWLLPEP
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIHVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNDKTRAIREQAMRKLDELY"
     gene            19486..20091
                     /gene="ynbA"
                     /locus_tag="ACHOSM_RS12450"
                     /old_locus_tag="pgaptmp_002490"
     CDS             19486..20091
                     /gene="ynbA"
                     /locus_tag="ACHOSM_RS12450"
                     /old_locus_tag="pgaptmp_002490"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU4558987.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHGTANHITLAALALSLLTGM
                     LLMLMSQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLFMSAER"
     gene            20091..20987
                     /gene="ynbB"
                     /locus_tag="ACHOSM_RS12455"
                     /old_locus_tag="pgaptmp_002491"
     CDS             20091..20987
                     /gene="ynbB"
                     /locus_tag="ACHOSM_RS12455"
                     /old_locus_tag="pgaptmp_002491"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU4558988.1"
                     /translation="MLEKSLVTLFALLILATLINHFLLWRLPERKGGEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLAGFLILATWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLQGLMGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDVGVKDSGKILPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            21003..22760
                     /gene="ynbC"
                     /locus_tag="ACHOSM_RS12460"
                     /old_locus_tag="pgaptmp_002492"
     CDS             21003..22760
                     /gene="ynbC"
                     /locus_tag="ACHOSM_RS12460"
                     /old_locus_tag="pgaptmp_002492"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HGU4558989.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGVKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKSSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            22750..24066
                     /gene="ynbD"
                     /locus_tag="ACHOSM_RS12465"
                     /old_locus_tag="pgaptmp_002493"
     CDS             22750..24066
                     /gene="ynbD"
                     /locus_tag="ACHOSM_RS12465"
                     /old_locus_tag="pgaptmp_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HGU4558990.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(24117..24722)
                     /gene="azoR"
                     /locus_tag="ACHOSM_RS12470"
                     /old_locus_tag="pgaptmp_002494"
     CDS             complement(24117..24722)
                     /gene="azoR"
                     /locus_tag="ACHOSM_RS12470"
                     /old_locus_tag="pgaptmp_002494"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HGU4558991.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            24923..28825
                     /gene="hrpA"
                     /locus_tag="ACHOSM_RS12475"
                     /old_locus_tag="pgaptmp_002495"
     CDS             24923..28825
                     /gene="hrpA"
                     /locus_tag="ACHOSM_RS12475"
                     /old_locus_tag="pgaptmp_002495"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HGU4558992.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARMVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARLSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            29097..29897
                     /gene="ydcF"
                     /locus_tag="ACHOSM_RS12480"
                     /old_locus_tag="pgaptmp_002496"
     CDS             29097..29897
                     /gene="ydcF"
                     /locus_tag="ACHOSM_RS12480"
                     /old_locus_tag="pgaptmp_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HGU4558993.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIVLLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            30094..31533
                     /gene="aldA"
                     /locus_tag="ACHOSM_RS12485"
                     /old_locus_tag="pgaptmp_002497"
     CDS             30094..31533
                     /gene="aldA"
                     /locus_tag="ACHOSM_RS12485"
                     /old_locus_tag="pgaptmp_002497"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGU4558994.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEWKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(31575..32576)
                     /gene="gap"
                     /locus_tag="ACHOSM_RS12490"
                     /old_locus_tag="pgaptmp_002498"
     CDS             complement(31575..32576)
                     /gene="gap"
                     /locus_tag="ACHOSM_RS12490"
                     /old_locus_tag="pgaptmp_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU4558995.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVLILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            32765..33295
                     /gene="cybB"
                     /locus_tag="ACHOSM_RS12495"
                     /old_locus_tag="pgaptmp_002499"
     CDS             32765..33295
                     /gene="cybB"
                     /locus_tag="ACHOSM_RS12495"
                     /old_locus_tag="pgaptmp_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HGU4558996.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            33540..33713
                     /locus_tag="ACHOSM_RS12500"
                     /old_locus_tag="pgaptmp_002500"
     CDS             33540..33713
                     /locus_tag="ACHOSM_RS12500"
                     /old_locus_tag="pgaptmp_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4558997.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            33739..33807
                     /locus_tag="ACHOSM_RS12505"
                     /old_locus_tag="pgaptmp_002501"
     CDS             33739..33807
                     /locus_tag="ACHOSM_RS12505"
                     /old_locus_tag="pgaptmp_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4558998.1"
                     /translation="MNPLVRCAGKIPALALTWTCRP"
     gene            complement(33785..33934)
                     /gene="hokB"
                     /locus_tag="ACHOSM_RS12510"
                     /old_locus_tag="pgaptmp_002502"
     CDS             complement(33785..33934)
                     /gene="hokB"
                     /locus_tag="ACHOSM_RS12510"
                     /old_locus_tag="pgaptmp_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HGU4558999.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT         8260 a         8067 c         9097 g         8581 t
ORIGIN      
        1 ttcggcgcgg gccacatgtg tggtgccggt ggtcatccag acaacggcgt cggtgttgtc
       61 cagcgactcg ttatccttac tgtattgtcc aagaccggtg tcatgagtag aacggttcgg
      121 atatttgcct tccgggaaac gctcgccagg atgataacgc gttacccaaa gctgcttgtc
      181 cataaagctt aaacgatgat agatccactc gtccggcgcg aactgggcac cttttgctac
      241 cgggtgagta ccacctgcat aaggaataat ttgataggaa actggattgc ccatgcggtt
      301 ctctttgttc gggttactca acagacgaat cgtgcccgga tcaaatttct gtgcggcatt
      361 ctgttcattg ccgatgttgt actgattaac ttgcatggta ctggtgcgtg ggccaccggc
      421 agtattcggt tttaccactg ggtccatcgc caccaggctg ttattctcgc catctacatc
      481 cagatcgagg cggaaattat aaatatgttg gtgtgtagta cccacgatat tgtgatcgat
      541 aagcgtgccg tagcgcgtgt catctttcgc cgtctcatcg tgcatggttt tcgctttaac
      601 acctttcacc gcttcgatgc ccgtagcacc ggcatcaatg ccaatagtgc cgttttcatg
      661 gaagatccag tcaaaaatgt agtcatagtt acccactgta ctgatccagc gcaccactaa
      721 ctcccggcgt tcggtactga cgttgggctg gcccatttcc tgatgcttat actcaggccc
      781 ggcataacgt tcaaataccg cgatagcgcg agggatctcc atcggcacgc cagtgtagtc
      841 ggcgatggtt tcattaagga gcactgcgtt agacggggca tctttaccac gagcaattgg
      901 tgaggttagc gtgcccatac cgtagtcacc agagtccaga tacgctttaa agtaccagcc
      961 aatatcagga tcaccgtaag gcacaatcat gccgccgaga gaaccttcgt acatgacttt
     1021 gcgtttggtg ccattgtcgt tataagtcac ggtggagatc atcggcccga cgcgagagtt
     1081 catgctgagg tgaaaatccc agttccgcca gtgaatcata tcgccagtaa tggtgtaatt
     1141 tttaccttca ggctcaatga tttgcatagg cttaactgcc ggagcaacgc ggtcacggcc
     1201 atcaaatggg cgtgcggtca ttggcaccgg aactaccgga ccttcttcaa tcttaacgat
     1261 ttttttctgt tctaaatcaa cgaccgccac caggttttcg atgggatgtg cccagtagtt
     1321 gccatcaccg acatcaagat agctgatgac tttgagcaac cgggcatctt gtttcaggcc
     1381 atctttacca tcgaaataac ctacggtcag cggcgtggta atcacttttt tcgcatcagt
     1441 aataccgcgt ttcttcacgg cagcggcaaa ttcttcactg ttgttaataa tgttctgcac
     1501 actggcgaaa tcatccagca acaccgtacc gtgggcgtct ttaatgggtt gccaggagag
     1561 cagtttgttg ttttgcagat ccaccaccgc ttcgatgata tgtttgccgt cgagcataat
     1621 gacgtcggct ttgcgcggct ggtcaaccgg tttgttttcc agcgcaaacg cccagacagc
     1681 ttctttatct ggcggtagca gggagatctc agtaaaacgg gtattgggtt tgaagtccgc
     1741 ggaagcttta acaatttcaa cggcctgttt aatttcgtcc gcagttagcg cattaagtgg
     1801 gtgagggcgc ttttctacct gaaaggtttg atccagcccg gactggaaaa catcgttaat
     1861 aaaggtgtca gaaacccagg ctttattgtc tttcatcact accggtactt gcagtgccag
     1921 aggctgacca ttaacaattg ctgtttgcgc accaggcttc actttcacgt acgcgccatc
     1981 tttaatcagg gtaaagatct gggcgtagtc gtcacactgc acatcggcac catatgcgct
     2041 tcaccaccgt gggcaaatac cggcgcttgc caggcgaaac ttaaggcgac tgccaacgcc
     2101 agggctgttt tacgggcaga atacagagag gggcttccca ttattaacct cgtcagatgt
     2161 tgtgttcttg ttagcaaccg cgctctgtgg gcggtttagt caggttcaca ttatcagcac
     2221 tgatgtaaag gggattgcct gcacctgcca ggttgtttgg caggtgtgcc agcttttcat
     2281 acagtgggtg ccctgaaaat agatgtacac atcatgcata atgtgacaac gtcacaaaat
     2341 ttggtgaaat aaaagggcaa ctattcgccg ttgcccttca ttcaccgatt aatcgacaaa
     2401 atcaccgtgc tgcctggcca ccagcgtcag aattgaatac agcgccaccg gggtttgatg
     2461 ctgattgaat acctctaccg cccattccac cacacctgtt ggtttttctt ctgcgctacg
     2521 ctgttttttc agcgtcttgc gcttacaggt gagacgcacc tggatggtat cgcctggctt
     2581 tacgggttcg ataaaacgca agctttccag cccgtagtta gcaatgaccg gaccgacacc
     2641 ggcatcgaca aacagacctg cagccgcaga aagcacaaaa tacccatgca caacccgctc
     2701 accgaaaata gattcggcag cagcaatctt atccatatgt gcatagaaat gatcgccgct
     2761 gaggcaagca aagttaacaa tatcggcctc tgtcattgtg cggcggggag tcagcaggct
     2821 gtcgcctggt tgtagctcct caaaatattt gcggaacgga tgaatacgat cttcttcgac
     2881 tttcgcacca cgcacccact gtttactgat agctgcaagc atcgtcggac taccctgaac
     2941 agcggttcgc tgcatgtaat gtttcaccgc tcgtaaacca cctaattctt caccgcctcc
     3001 tgcgcgacca ggcccaccat gtaacagttg tggcagtggg gagccatgcc cggtggattc
     3061 ttttgccgac tcttcattga ggatctgaat tcgcccatgc gtacgtgccg cgtcggcaat
     3121 aaactgacgc gcaatttgcg gatcagccgt caccagcgtt cccgcaaggc taccgccgcc
     3181 tgcacaagcc agttgcagag catgttgctg gttttgtgct ggcattagcg ttgcgacagg
     3241 gccaaaggct tctgttgcat gtaccgccgg tgtttcatcc ggctgcggac agtacaataa
     3301 ggttggcggg aagaaggcac ccgcagcaga taaatccgcc tgaccaccga ggcgaatctc
     3361 gcatcctgca gccagcaata tgttcacttt ttcctgcaca tcggcacgct gctcagcatt
     3421 taccagtgcg cccattttca cgccttcctg agcaggatca ccgaccacga ctttctgtaa
     3481 tcgcgcaacc agagcatcac tgacagcatt aaccaatgcc tgcggcacaa taatccgccg
     3541 gattgccgta catttttgcc cggcttttgt ggtcatctca cgcacaactt cacgaataaa
     3601 cagcgcaaac tccggttgat ccggggtgac atcttcgccc agtacgcagc agttcaggga
     3661 atcagcttcc atagtgaagg ggatagattt ggcgacgata tttggctgaa ctcgcagcat
     3721 ctgtccggtc gccgctgacc ccgtgaaagt caccacatcc tggctgtcca gatgatccaa
     3781 caagtcgcca gcactaccgc agatcagact aattgcacct tcgggaacaa gaccactatc
     3841 gacaattgat ttcaccatcg cctgagtcag ttgggccgtc gcggtagctg gtttgatgat
     3901 ggctggcatt ccgcccagcc acgttggtgc cagcttttcc agcattcccc agcaggggaa
     3961 gttaaaggcg ttaatatgca ctgccacgcc tgactttgag gtcagtacat ggcgcgcggc
     4021 aaatccacct tctttcgata aggggatcaa ttcatcttcc ggccacagcg tatcgtcagg
     4081 cagctcccgg ctaccgaggc tggcgtaagt aaataacgtc ccaatgccac cttcaatatc
     4141 aacccaactg tctgcccgcg ttgcgcctgt ttgcgcagaa agagcataga agcgctcttt
     4201 ttcactcagc agatgtttag cgaccgcttt aagcatcgcc gcacgttcga taaaggtcat
     4261 agcgcgaagg gctggggcac ctttttcaat ggcaaactgg cgggcagccg ccatatcaag
     4321 accttcactg ctcacttccc ataacgcctc gccgctaata gcgtggtgaa tcaaacggct
     4381 acggccccgg ccagactgcc aggtaccgga taagaaactg gctaactgct gcatcgctac
     4441 tctccagatg tttcacattt ctgttgctaa tagttaaatc gcgaatcata aaaagcaagg
     4501 gatcttttaa cgaaatgtta actatgcgat ctgtatagca actgcggaaa acattaatgc
     4561 actgataaat aatgatttat aaaaatgggg tgcgaaatcc gtcacagttc aagcatacaa
     4621 aatttgtgat tttagttaac cattgtgtaa cttttataaa acaatgtgat tcgtgttttt
     4681 aattaattca cgaaaactgg aatcgtaaag gtgatgacgt gacccaagaa gaacgctttg
     4741 agcaacggat agcccaggaa acggctatcg agccacagga ctggatgccc gatgcttacc
     4801 gaaagacact gatccgccag attgggcagc atgcgcactc cgagattgtt ggtatgttgc
     4861 ctgaaggtaa ctggaacact cgcgcaccaa ccttgcggcg taaagccatt ctgttggcca
     4921 aagtgcagga tgaagccggt catggtctgt atctctatag cgccgccgaa acactgggct
     4981 gcgccagaga agacatctac caaaaaatgc tcgacggacg gatgaaatat tcctccatct
     5041 ttaattatcc gacattgagt tgggctgata tcggcgttat cggctggctg gtggatggcg
     5101 cagcgatcgt caatcaggtg gcgttatgcc gcacttctta tggcccgtat gccagagcaa
     5161 tggtgaaaat ctgcaaagaa gagagttttc accagcgtca ggggtttgaa gcctgcatgg
     5221 cactggcgca gggcagcgaa gctcaaaagc agatgttgca agacgccatt aaccgtttct
     5281 ggtggccagc cttaatgatg ttcgggccaa acgatgataa ctcgccaaac agcgccagaa
     5341 gtctcgcctg gaaaatcaaa cgtttcacca acgacgaact ccgccagcgt ttcgtggaca
     5401 acaccgttcc acaggttgaa atgctcggta tgaccgttcc tgacccggat ctgcattttg
     5461 acactgaaag cggtcactac cgctttggtg agatcgactg gcaggagttt aacgaagtaa
     5521 ttaacggtcg cggaatttgt aatcaggaga ggctcgacgc caaacgtaaa gcctgggaag
     5581 aaggtacctg ggtacgggaa gcagcgctgg cccatgcaca aaaacaactt gcccgtaagg
     5641 tcgcataagg agattcaaaa tgagtaatgt ttactggccg ttatacgaag ttttcgtgcg
     5701 tggcaaacag ggcttatcac accgccatgt tggcagttta catgctgccg atgagcggat
     5761 ggcactggaa aatgcccgtg atgcttacac ccgtcgtagc gaaggatgtt caatttgggt
     5821 ggtgaaggcg agtgaaattg ttgcctcgca accggaagaa cgcggtgaat tttttgatcc
     5881 ggctgaaagc aaggtctatc gccatccaac gttttacacc atccctgatg gcattgagca
     5941 catgtgaggt cggaaatgaa tcagttaacg gcttacacct tgcgcctggg cgataactgt
     6001 ctggtgctct cccagcggtt gggggaatgg tgcggtcacg caccggaact ggaaatcgat
     6061 ctcgcactgg caaacattgg cctcgattta ttaggtcagg cacgtaactt cttatcgtat
     6121 gccgctgaat tagcgggaga aggtgatgaa gataccctgg ccttcacccg agacgagcgc
     6181 cagttcagca acttattgtt ggttgaacaa ccaaacggca attttgccga caccattgca
     6241 cgccagtatt tcatcgatgc atggcatgtg gcgctcttta cccgtctgat ggaaagccgc
     6301 gagccgcaac tggcggcgat ttctgccaaa gcaattaaag aagcgcgtta tcacctgcgt
     6361 tttagtcgtg gctggctgga gcgactgggc aatggtactg acgtatcagg gcaaaagatg
     6421 cagcaggcca tcgacaagtt gtggcgtttt accgccgaac tgttcgatgc cgacgagatt
     6481 gatattgcac tgagtgaaga gggtattgtg gttgatccac gcactttacg cgcagcgtgg
     6541 gaagctgaag tttttgccgg gatcaacgaa gccacattga acgtaccgca agagcaggcg
     6601 tatcgcactg gcggtaaaaa aggactgcat accgaacacc ttggacccat gctggcagaa
     6661 atgcagtatc tccagcgtgt cttgcccggt cagcaatggt aaccgaggag atgggtatgc
     6721 aacgtctggc caccattgca ccgccgcagg tgcatgagat atgggcgcta ctcagccaga
     6781 tcccggaccc ggagatcccg gtgctgacca ttaccgattt aggcatggtg cgtaatgtga
     6841 cacagatggg agaaggatgg gtgatcggct ttacgccgac atattccggt tgtccggcaa
     6901 cggaacattt gattggcgcg atacgtgagg caatgtcaac acacgggttt acccccgttc
     6961 aggttgtgct gcaacttgac ccggcatgga ccaccgactg gatgaccccc gatgcccgtg
     7021 agcgtctgcg agagtatggc attagcccgc ccgccggaca cagttgccat gcccatttgc
     7081 cgccagaagt acgttgcccg cgctgcgcca gcgtccatac cacacttatc agtgaatttg
     7141 gttccacggc ctgcaaagca ttgtaccgct gcgatagttg ccgcgaacct ttcgattatt
     7201 tcaaatgtat ttgaggatgc catgacaacg tttcattcct taacagtggc aaaagtcgag
     7261 ccggaaaccc gtgatgcggt gaccattacc tttgcggtgc cccagccttt gcaggaggcg
     7321 tatcgctttc gccccggtca acatttgacc ttaaaagcca gctttgatgg tgaagaatta
     7381 cgccgttgtt actccatttg ccgcagctac ctgcctggcg aaattagtgt ggcggtaaaa
     7441 gccattgaag gcggacgttt ctcccgctat gcccgcgaac acatccgcca gggaatgacg
     7501 ctggaagtca tggtgccgca ggggcatttc ggctatcagc cgcaggccga acgccagggc
     7561 cgctatctgg ctattgcagc aggatcaggt attacgccaa tgctggcgat tattgccacc
     7621 actttacaaa ccgagcctga aagtcagttc accctgatct acggtaaccg taccagccag
     7681 agcatgatgt ttcgccaggc actggcagac ctgaaagaca aatatcctca gcgtttacag
     7741 ttgttgtgca ttttcagtca ggaaacactc gacagcgatc tgcttcacgg gcgtattgac
     7801 ggtgaaaaat tacagtcact tggggcctcg ctcattaatt ttcgtcttta tgatgaagcg
     7861 tttatttgtg gtccggcggc gatgatggat gacgcggaaa ccgctttaaa agcactggga
     7921 atgccagaaa aagcgattca tctggagcgg tttaatacgc ctggcacgcg cgtcaaacgt
     7981 agcgttaacg tgcaaagtga cggacaaaaa gtgactgtac gtcaggatgg gcgggatcgg
     8041 gaaatcgtgc ttaatgccga cgatgaaagc attctcgatg cggcattgcg ccagggggcg
     8101 gatctgccct atgcctgcaa aggcggcgtc tgtgcgacct gcaaatgcaa agtgctgcgt
     8161 ggcaaagtgg cgatggaaac caattacagt ctggaaccgg atgaactggc tgcaggctat
     8221 gtgttgagtt gccaggcact gccgctgacc agcgatgtgg tggttgactt tgacgcgaag
     8281 gggatggcat gagcgaactg atcgtcagcc gtcagcaacg agtattgttg ctgaccctta
     8341 accgtcctgc cgcacgtaat gcgctaaata atgcccttct gacgcaactg gtaaatgaac
     8401 tggaagctgc ggctatcgat agcagtattt cggtctgtgt gattaccggt aatgcacgct
     8461 tttttgccgc cggggcagat cttaacgaaa tggcagaaaa agatctcgcg gccaccttaa
     8521 acgatacacg tccgcagcta tgggcgcgat tgcaggcctt caacaaacct ctcatcgcag
     8581 ccgtcaacgg ttacgcgctt ggtgcgggtt gcgaactggc attgttgtgc gatgtggtgg
     8641 ttgccggaga gaacgcgcgt tttggtttgc cggaaatcac tctcggcatc atgccaggcg
     8701 caggaggaac gcaacgttta atccgtagtg tcggtaaatc gttagccagc aaaatggtgc
     8761 tgagcggaga aagtatcacc gctcagcaag cacagcaggc cgggctggtt agcgacgtct
     8821 tccccagcga tttaaccctc gaatacgcct tacagcttgc gtcgaaaatg gcacgtcact
     8881 cgccgctggc cttacaagcg gcaaagcaag cgctgcgcca gtcgcaggaa gtggctttgc
     8941 aagccggact tgcccaggag cgacagttat tcaccttgct ggcggcaaca gaagatcgtc
     9001 atgaaggcat ctccgctttc ttacaaaaac gcacgcccga ctttaaagga cgctaatgat
     9061 ggaattcatc ctcagtcatg tagaaaaggg cgtgatgaca ctaacgctca accgcccgga
     9121 acgcctgaac agttttaatg atgagatgca cgcacaactg gcagagtgtc tgaaacaggt
     9181 cgagcgcgac gacactatcc gttgcctgtt acttacaggt gccgggcgcg gattttgtgc
     9241 tggtcaggat cttaacgatc gtaacgttga tcccacaggc cccgcaccgg atttagggat
     9301 gtcagttgaa cgtttctata acccactggt acgtcgcctg gcaaaactgc caaaaccggt
     9361 gatctgtgca gtcaatggcg tggcggcagg ggctggcgca acactggcac tgggatgcga
     9421 catcgttatt gctgcccgtt cagcaaaatt cgtcatggcg tttagtaagt taggcttagt
     9481 acccgattgc ggtggaacct ggttactgcc acgcgttgct gggcgagcgc gcgccatggg
     9541 gctggcactg ctggggaatc aactgagtgc tgaacaggcg cacgaatggg ggatgatctg
     9601 gcaggttgtt gatgatgaaa cgctggcaga taccgcgcaa cagctggcac ggcatctggc
     9661 gacacaaccg acatttggtc ttggacttat caagcaagcg ataaatagcg ctgaaaccaa
     9721 tacgctcgat acgcaactgg atctggaacg cgactatcag cgacttgccg gacggagcgc
     9781 agattatcgt gaaggtgtca gtgcgttcct ggctaaacgc tcaccgcagt tcacggggaa
     9841 atagcatgat gataaatgtg caaactgtgg cagtgattgg gagcggcacc atgggggctg
     9901 gcattgctga agttgccgcc agtcatggac atcaggtttt actgtatgac atttctgctg
     9961 aagcgctgac ccgcgcaatc gacgggatac acgcgcggct aaattcacgc gtgacgcggg
    10021 gaaaactgac tgctgaaacc tgtgaacgca cattgaaacg cctgatcccg gtgaccgata
    10081 ttcacgcgct ggcagctgcg gacctggtca ttgaagcggc gtctgaacgt ctggaagtca
    10141 aaaaagcgct ctttgcacag ctggcggaag tttgcccgcc acaaacgcta ttgaccacta
    10201 acacttcgtc aatctctata accgcgattg ctgcggaggt aaaaaatcct gaacgtgttg
    10261 cggggctgca tttttttaac ccggcaacgg tgatgaagtt ggtggaggtg gtcagtgggc
    10321 tggcaacggc agcggaagtt gttgagcagt tgtgtgaact aacgttgagt tggggtaagc
    10381 agcctgtgcg ctgtcattcg acccctggat ttatcgttaa ccgtgtggcg cgtccttatt
    10441 attccgaggc ctggcgggca ctggaagagc aggttgctgc accagaagtg attgacgctg
    10501 cacttcgcga tggcgctggt ttcccgatgg ggccgctgga attaaccgat ctgattggtc
    10561 aggacgtcaa ttttgctgtc acctgttcgg tgtttaacgc tttctggcag gagcgtcgtt
    10621 ttttaccttc gctggtgcaa caggaactgg tgattggtgg acggttgggc aagaaaagtg
    10681 ggctgggcgt gtacgactgg cgcgcggaac gtgaggcagt tgttggcctg gaagcggtaa
    10741 gcgacagttt tagcccaatg aaagtagaaa agaaaagtga cggtgtcacg gaaattgacg
    10801 atgttttatt gattgagaca caaggcgaga cggcacaggc gctggcaata cgactggcac
    10861 gcccggtggt agtggtcgac aaaatggcgg gcaaggtggt gaccattgct gcggcagcgg
    10921 tgaacccgga ctcagcgacc cgcaaggcca tttattacct gcaacagcag ggcaaaacag
    10981 tgctgcaaat tgcagattac ccaggaatgc tgatttggcg aacggtagca atgatcatca
    11041 atgaagccct tgatgcgctg caaaaaggcg tggcctctga acaggatatc gataccgcca
    11101 tgcgtcttgg ggtgaattat ccatatggcc cgcttgcctg gggagcgcaa cttggctggc
    11161 agcgaatatt aaggctcctg gaaaatctac aacatcacta tggcgaagaa cgctatcgcc
    11221 catgttcatt gctgcgccaa cgagcgcttc tggagagcgg ttatgagtca taaggcctgg
    11281 caaaatgccc atgcaatgta tgagaacgat gcctgcgcca aagcgcttgg catcgacatt
    11341 atctcaatgg atgaaggctt tgctgtagtg accatgaccg tcactgcaca aatgcttaac
    11401 ggtcatcaaa gttgccacgg cgggcagcta ttttcactgg ctgatactgc ctttgcctac
    11461 gcctgcaata gccaggggct ggcagccgtc gcttctgcct gcacgattga ttttttacgt
    11521 ctaggctttg ccggagacac cttaactgcg actgcgcagg ttcgtcatca gggcaagcaa
    11581 accggtgttt acgacatcga aattgttaac caacaacaaa aaacggttgc gctgtttcgc
    11641 ggtaaatctc accgcatcgg cggcaccatt acaggagaag cctgatgcgt gaagccttta
    11701 tctgtgacgg aattcgtaca ccaattggcc gctacggcgg ggcattatca ggtgttcggg
    11761 ctgatgatct ggctgctatc cctttgcggg aactgctggt gcgaaacccg cgtctcgatg
    11821 cggaatgtat cgatgatgtg atcctcggct gtgctaatca ggcgggagaa gataaccgta
    11881 acgtagcccg gatggcgact ttactggcgg ggctgccgca gagtgtttcc ggcacaacca
    11941 ttaaccgctt gtgtggttcc gggctggacg cactgggggt cgccgcacgg gcgattaaag
    12001 cgggcgatgg cgatttgctg atcgccggtg gcgtggagtc aatgtcacgg gcaccgtttg
    12061 ttatgggcaa ggcagccagt gcattttctc gtcaggctga gatgtttgat accactattg
    12121 gctggcgatt tgtgaacccg ctcatggctc agcaatttgg aactgacagc atgccggaaa
    12181 cggcagagaa tgtagctgaa ctgttaaaaa tctcgcgaga agatcaagat agttttgcgc
    12241 tacgcagtca gcaacgtacg gcaaaagcgc aatcctcagg cattctggct gaggagattg
    12301 ttccggttgt gttgaaaaac aagaaaggcg ttgtaacaga aatacaacat gatgagcatc
    12361 tgcgcccgga aacgacgctg gaacagttac gtgggttaaa agcaccgttt cgtgccaatg
    12421 gggtgattac cgcaggcaat gcctccgggg tgaatgacgg agccgctgcg ttgattattg
    12481 ccagtgaaca gatggcagca gcgcaaggac tgacgccgcg ggcgcgtatc gtagccatgg
    12541 caaccgccgg ggtggaaccg cgtctgatgg ggcttggtcc ggtgcctgca actcgccggg
    12601 tgctggaacg cgcggggctg agtattcacg atatggacgt gattgaactg aacgaagcgt
    12661 tcgcggccca ggcggtgggt gtactacgcg aattggggct gcctgatgat gccccacatg
    12721 ttaaccccaa cggaggcgct atcgccttag gccatccgtt gggaatgagt ggtgcccgcc
    12781 tggcactggc tgccagccat gagctgcatc ggcgtaacgg tcgttacgca ttatgcacca
    12841 tgtgcatcgg tgtcggtcag ggcatcgcca tgattctgga gcgtgtttga gcatatcaac
    12901 ctgcgagtac cctacaatga taaccaatac aaagcttgac ccgattgaaa ccgcgtctgt
    12961 tgatgagtta caggcgttgc aaacacagcg tctgaaatgg acgctcaaac acgcgtatga
    13021 aaatgtcccg atgtatcggc gcaaattcga cgcagcaggc gtacatcctg atgatttcag
    13081 ggaactttca gacctgcgta aatttccctg taccaccaaa caggatctgc gcgacaacta
    13141 tccctttgac acctttgccg tgccgatgga acaagtggtg cgcattcatg cttcttcggg
    13201 aaccacaggt aaaccgacag ttgtcggtta tacgcaaaac gatattgata actgggccaa
    13261 tattgtagcg cgttctttgc gtgcggcagg gggctcgccc aaagacaaaa ttcatgttgc
    13321 ctatggttac ggcctgttta ctggtgggct gggtgcgcac tatggtgccg aacgtctggg
    13381 cgatacggtg atcccgatgt ctggcgggca gacggaaaaa caagcgcaac tgatccgcga
    13441 ttttcaacca gatatgatca tggttacgcc atcttattgc cttaacctga ttgaagagct
    13501 ggagcggcag ttgggcggtg atgccagcgg ttgctcgctg cgggttggcg tgtttggtgc
    13561 tgagccgtgg acacaggcca tgcgtaaaga gattgaacgg cgtctgggga tcaccgcact
    13621 ggatatttat ggcctgtcag aagtgatggg gccgggggtg gcgatggagt gtctggaaac
    13681 taccgacggt ccaactattt gggaagatca tttctatcct gaaattgtta atcctcatga
    13741 cggcacaccg cttgccgatg gtgaacatgg cgaactgtta ttcaccacgc tgaccaaaga
    13801 agcattgccg gtcattcgtt accgcacgcg tgatttaacc cgactgttac caggaacggc
    13861 gcggactatg cgccgcatgg atcgcatcag cggacgcagc gatgacatgt tgattattcg
    13921 cggtgtaaat gtctttccgt cacaactgga agaagagatt gtcaaattcg aacatttatc
    13981 gccgcattac caactggagg tgaaccgccg ggggcatctt gattcacttt ctgtgaaagt
    14041 ggagttgaaa gaaagtagcc tgacattgac gcatgagcag cgttgccagg tatgccacca
    14101 gctgcgccat cggattaagt cgatggtggg gatctctacc gatgtgatga tcgttaactg
    14161 tggcagtatc ccgcgttcag aaggcaaggc gtgtcgggtg tttgatctgc gcaatattgt
    14221 tggtgcctga tgtgtcgtct ggcccgggtg gggtaaagcg ccagggccag aagtcgatac
    14281 gacctgtgct atgattcata aatcacaaca ataacaacag actgaatcga atgagtaaac
    14341 ttgatacttt tatccaacat gctgtaaacg ctgttcctgt cagtggcaca tctttgatct
    14401 cctctctgta tggtgattct ctttcccatc gtggtggtga aatctggctg ggtagtctgg
    14461 ctgctttgct ggaagggctg ggatttggtg agcgtttcgt gcgcactgct ttgtttcgtc
    14521 ttaataaaga aggctggctg gatgtttccc gcatcgggcg acgcagtttc tatagcctca
    14581 gtgataaagg cttgcgcctg acgcgacggg cagaaagtaa aatttatcgc gcagagcaac
    14641 ccgcatggga tggtaaatgg ctcctgttgc tctcggaagg tttagataaa gcgacgctgg
    14701 ctgatgtcaa aaagcagttg atctggcaag gttttggtgc actggcgccc agcctgatgg
    14761 catcgccgtc gcaaaaactg gccgatgtac agaccctttt gcatgaagcg ggtgtggcgg
    14821 ataacgtgat ttgttttgaa gcgcaaatac cactggcgct ttctcgtgca gcactgcgtg
    14881 ccagagtaga agagtgctgg catttaactg aacaaaatgc catgtacgaa acctttattc
    14941 agtcattccg cccgctggtg ccgctgttaa aagaggcggc agacgagtta accccggagc
    15001 gggcatttca tattcagctt ttactgatcc atttttatcg ccgtgtcgtc cttaaagacc
    15061 cattgttgcc ggaggagttg cttccggcac actgggcagg gcatacggcg cgtcagctgt
    15121 gtatcaacat ttatcagcgc gtagcgcctg ctgctttagc gttcgttagt gaaaaaggtg
    15181 aaacctcggt cggtgaactg cctgcgccgg gaagcctgta ttttcaacgt tttggcggct
    15241 tgaatattca acaggaggcg ttatgccaat ttatcagata gacggtctga ctccggttgt
    15301 gccagaagag agttttgttc atccgacagc ggtattgatc ggcgatgtta ttctcggcaa
    15361 gggtgtttac gttgggccaa atgccagcct gcgtggcgat tttggtcgta tcgtggtgaa
    15421 agatggcgcg aacattcagg ataattgcgt tatgcacggt tttcccgagc aggatactgt
    15481 cgtggaagaa gatggacata ttggtcatag cgctatcctt cacggctgca ttatccgccg
    15541 caatgcatta gtgggaatga acgcggtatt gatggacggc gcggtgattg gcgagaacag
    15601 cattgttggc gcatccgcat ttgtgaaagc caaagcagaa atgcctgcca attacctgat
    15661 tgtcggcagc ccggcgaaag cgattcgtga actcagtgag caggagctgg catggaaaaa
    15721 gcagggtacg catgagtatc aggtgctggt gactcgttgt aagcagacgt tgcatcaagt
    15781 tgagccattg cgggaagttg aaccgaacag gaaacgtctg gtatttgatg agaatctgcg
    15841 accgaaacag taacgtccag atactgaaca ttctaatttt tagcatgtga tacctaaacc
    15901 gcaacgctga tacaggttgc ggttttttta ttgccggatg cggcgcttta cgcgttttgt
    15961 tttggtgtct ttcaggacaa tagagcaact catccagtaa tcttgtttac accttcgcat
    16021 tatttatctc ttttcatttc tatactgatt tttcttaatc cgttttatta cagggcaggg
    16081 tgcgatgagc agcaatacat ttactctcgg tacaaaatcc gttaaccgtc ttggttatgg
    16141 cgcgatgcaa ctggcaggtc ctggggtttt tggcccccca agagatcgcc acgtcgctat
    16201 aactgtgctg cgtgaggcgc tggcattggg cgtcactcac attgatacca gcgactttta
    16261 tggtccgcac gtcaccaatc agattatccg cgaagcgctt tatccttact ctgacgacct
    16321 gacaattgtc actaaaattg gtgcgcggcg tggagaggac gcttcctggt tgcccgcctt
    16381 ttctccggca gagttgcaaa aagcggtgca cgataatcta cgtaatctcg gcctggacgt
    16441 actggatgtg gttaacctgc gcgttatgat gggagatggt catggcccag cggaaggatc
    16501 gattgaggcc agcctgaccg tgctggcaga gatgcaacaa caaggcctgg taaaacatat
    16561 tggcctgagc aacgtcacac cgacgcaggt tgcagaggcg cgcaagattg ccgaaattgt
    16621 ctgtgtgcaa aacgaataca acatcgcgca ccgtgctgat gatgcaatga ttgatgcttt
    16681 ggcccacgat ggcattgcct acgtgccgtt cttcccgctc gggggcttta caccgctgca
    16741 atcctctacg ctgtcggatg ttgctgcgag cctgggtgca acacccatgc aggtggcgct
    16801 ggcgtggctg ttacaacgtt caccgaatat tttgctgatc ccagggacgt cttctgttgc
    16861 gcatttacgg gagaatatgg ctgctgaaaa attgcatctt tctgaggaag tgttgtctac
    16921 gttggatggt atttcgcgag aataacgaat atacaaaagg gaaagatgca tttccctttt
    16981 gtttcttttt taatggcatg gagtgcatat gtttaaggcc aggatttgcg gctggatagg
    17041 tttacttccc ctgtttatgc tgagtcttcc agtacaggcg gaacttcgat gtgttgcaaa
    17101 tacagttgat attgaaccat ttttttctgc ggctaccgct gaagataaac aacaagttga
    17161 acaagctatc aacagtagcg tgaatcttgt ccccttcggt ttatctgcat cggactggaa
    17221 agtgcatcgt ggcgatttag tggtagaagg taatatagag agtaatcaaa aattgattgt
    17281 tcttggtaat ttgacagtca aaggtaatat ttccactttc tctctttcta atccatgggt
    17341 tattctcggt aacgtgacgg caacgaatat agttacggat tcaccactgt taattaccgg
    17401 ttcgataaat gcaagtggac tggtatttat cgactcatat tacgataatc cgtctacgat
    17461 taaggggagt attaatgcgc gtgggatatt tatcaatgac ataattgcac ctgttgttgc
    17521 gtcttcgaca aatagtgaat tcatggtccg tgcgagtgac aaaaatgaca ctgaaaatgt
    17581 caaaaaagcg ctgatgataa taaatcctga tgcatattat tggggtctaa taaatgatga
    17641 agatgctctg aaagaaattt ttaagcgaag caatattcgc atggcaggga atgtctgtaa
    17701 tcagatgaaa aaagaagcgc tgtttcgccc taagccttct cctgagttag tgcaggaatt
    17761 gcaaatgctg gatgaaggca acgttgctgc atttgaagga cgagatattg cgacatttga
    17821 tcttgccata atgcgaactc tcccaaggct taaaggaatt tcagctaacc tacgcaaaca
    17881 acttattaat agcaatgatg aacaaacgat tgaaagtatg gcgagatata tgcctgacaa
    17941 tgagattctg gagttgaccg atcagcaatt gggttaccag cctgttgttc tggggttgct
    18001 cgaccgtgaa ccgctctctg tcgaaataat gacgcgaatg agtcgtctac ctgatggtgt
    18061 cggtccgttg aatcttgcgc tacgtgaaaa tctccctctg gatatcgtaa ttactctggc
    18121 gaaaagagat tgggatatga tcattcagga gctttataaa gatgcgtggt tattgcctga
    18181 acccattatt gatggctata tccgcagtga tgattcctct attcgtcagg tcggtgctgg
    18241 aggacaactc acctacaatc aggcaatgca gttggcgaac gattcatcaa acaatgttgt
    18301 cacaagctta gcgttcaagc tggcagagat gaaacaccat ggtcaattat tgcggatgac
    18361 gccacaagag agtgacaaag ttgcagccta tttataccaa aaattcgaga atgacgatga
    18421 tctaatacat gtgttatttt tagcattgcc agataactta cagtttaatt ttgttaaaag
    18481 gatggagaaa aaatcccctg cctacttttg ctgtcgggat atgcaggtaa ttcactctga
    18541 cgctgctcta caacggttat tgacacgttt caacgatcct gaggggtgga gtaatctggc
    18601 gaaaaatcag tatctgagca catcgatgaa acaaaaaatt tggcaacgtg ctttatcgca
    18661 tcggaaaaat aatccgaaag cagattcagc tgcgtatgaa accagcgctg atatgatttt
    18721 gtctgagctt atcagccacg gtgaagttga tgatcagatg ctattaaatg ccaccgccct
    18781 gatacgtttg gaagactggg actttttaga gagtgcatta gttagttggg ataatttacc
    18841 cgctgttgtc ctcaaggaat tacagcaaaa tacgccacgc aatgatattt gggcgaagtt
    18901 ttttctgagg caggaaaaca gctcccgtgc gcaggttgat gaagcattac gcgtctatta
    18961 tgcactcgac cccgatgcgt tagcacaact ggatgtactg gcaaaacaac cggatcgtat
    19021 atggtggagt acactggcga aaagcaatct cacatttttc aagttcggcg cacttaacaa
    19081 ccgccacaca ccgcctgcag tactggcggc agaaattgat cccgagtggt ggattgtggc
    19141 gatgaataat ccccgttttc cagttgatgt attaaaggcg aggctgaaac gtgatccttt
    19201 gctggcgtta gagcttgtta accctgaact ggatttagtc cgccagctgg cgcttaacga
    19261 taagacgcgc gcaatacggg aacaagcgat gagaaaactt gatgagttgt attgatccac
    19321 aacgtaatgt ttcttaacca tttgattaat tggggataat cattcctggc agtgagtccc
    19381 caataccttg atatattctg aatttttaat gaagcggcgt gttgcgatat ctccgtcagg
    19441 ggaattgatg caccatagcg caaaccgaat tatcaaggat tgataatgac gctctaccag
    19501 ataaaaccgc tctttcagtc gctgttaagg ccgacgatgt tttggcttta taagcaccac
    19561 ggtacagcga atcacatcac tcttgctgca ctggcgcttt ctcttcttac cggtatgctg
    19621 ttgatgttga tgtcacaacc catcctcttt atgctattgc ccatcgtgct ttttatccgt
    19681 atggcgctca atgcgcttga tggcatgttg gcgcgtgagt gcaaccagca aacacgtctg
    19741 ggggcgattt tgaatgagac tggcgatgtt atttccgata ttgcgctcta tttaccgttt
    19801 ttatttttac cggaaagtaa cgcatcactc gtaatactca tgttattttg caccatattg
    19861 actgagtttt gcggtttact cgcgcagacg attaacggta ttcgcagtta tgccggtcca
    19921 ttcggcaaaa gcgatcgtgc gttaatattt ggtttgtggg ggctggccgt tgccatttat
    19981 ccacagtgga tgcaatggaa taatctttta tggagcattg cttcaatact gcttctctgg
    20041 actgcgatta atcgttgtcg gagtgtgctt tttatgagtg ctgaaagata atgctggaaa
    20101 aatctctggt aacacttttc gcgttgttaa ttttagcaac gctgataaac cattttctgc
    20161 tatggcggtt accggagaga aaagggggtg aggttacatt acgtattcgt acctggtggg
    20221 gcatcgtcat ttgtttttca ctggtgattt caggtccacg ctggatgacg ttgacgtttt
    20281 ttgcgctgat aagttttctg gcattgaaag aatattgtac gcttatatcc gtacattttt
    20341 cgcggtggtt atattgggtt attcctctta actatttgct tatcggtttt aattgctttg
    20401 agctgtttct gttatttata cctttggctg ggtttctgat attagccacc tggcgagttt
    20461 ttgtgggtga cccctccggt tttctgcata ccgtgagcgc cattttttgg ggctggataa
    20521 tgaccgtttt cgccttgagt catgccgcct ggttattaat gttgccaacc acaaatatcc
    20581 agggcggggc gttactggtg ttatttcttt tggcattaac cgagtcaaac gatattgcac
    20641 agtatttatg gggaaaatcc tgcggcagaa gaaaagtggt ccccaaagtc agcccgggaa
    20701 aaacactgca aggtctgatg ggtggcgtaa tcaccaccat gattgcgtca ctgattattg
    20761 gaccgttgct gacaccgcta aatacattac aggcattatt agcgggtttg ttaattggta
    20821 ttagtggttt ttgcggcgat gtagtgatgt cagccatcaa acgagatgtt ggtgttaaag
    20881 atagtggaaa aatattacca ggacatggcg gactccttga caggattgac tcattaattt
    20941 tcaccgctcc ggtatttttt tattttatac gctactgctg ttactgaagg aaaatagaga
    21001 aaatggaaaa ttcacgcatc cctggggaac atttttttac caccagtgat aatacagcgt
    21061 tgttttatcg gcactggccc gctttacagc ccggggtgaa aaaggtcatc gtcttatttc
    21121 atcgcgggca tgaacattct ggtcgtctac aacatctcgt tgatgaactg gcgatgccag
    21181 atactgcttt ttatgcctgg gatgcccgag ggcatggaaa aagttcgggg ccgcgtggtt
    21241 atagcccatc tcttgcgcgt tcagtgcggg atgtcgatga atttgtccgt tttgctgcca
    21301 gcgacagcca ggttggactg gaagaggtgg ttgtgatcgc gcaaagcgtc ggcgcagtgc
    21361 tggttgccac atgggttcat gattatgcgc ctgcaattcg cgggctggtg ctggcttctc
    21421 cggcctttaa ggttaaattg tatgtgccgc tggcacgtcc tgcgctgacg ctatggcatc
    21481 gtctgcgtgg tctgtttttt attaattcct atgtgaaagg acgctatttg acccacgatc
    21541 ggcaacgggt ggcgagtttc aataatgatc cgctgatcac acgggcgatt gccgtcaata
    21601 tcttgctcga tctctataaa acgtctgaac gtattattag agatgcggcg gcgattacgc
    21661 tccccacgca acttctgata tcaggcgatg actatgtggt gcatcgccaa ccgcagattg
    21721 atttttatca gagattacgt agccctctga aagagctgca tctgctgcca ggcttttatc
    21781 acgacacgtt gggtgaagag aacagggcgc aggcatttga aaaaatgcaa agctttatta
    21841 gtcgtttata tgctaacaag tcacaaaaat ttgattatca gcatgaagac cgcactggac
    21901 catcagcgga tcgctggcgc ctcctttcag gtggacccgt gccattatcg ccggttgatt
    21961 tggcgtatcg ctttatgcgt aaagcgatga aattgttcgg gacgcactct gcgggcctgc
    22021 atctcggaat gagcactggc tttgattcag gcagttcgct ggattatgtc tatcaaaatc
    22081 aaccgcaagg cagtaacgca ttcgggcgct taatcgacaa aatctacctg aacagtgttg
    22141 gctggcgcgg tattcgccag cgcaaaaccc atttacaaat actgattaaa caagccgttg
    22201 ccgatctcca cgccaaaggt ttagccgtcc gcgtggttga cattgccgca gggcatgggc
    22261 gctatgtact ggatgcactg gcaaacgagc ctgccgtaag cgatatttta ttacgtgatt
    22321 acagtgagtt aaatgttgca caggggcaag agatgattgc ccaacgggga atgtctgggc
    22381 gggtgcgttt tgaacagggc gatgcgttta acccggagga actcagcgcg ttaactccgc
    22441 ggcctacgct ggcgattgtc tctggactgt atgagctgtt tcccgaaaat gagcaggtaa
    22501 aaaactcact cgcaggtctt gccaatgcca tcgaaccggg cggcattctc atctacaccg
    22561 ggcagccgtg gcaccctcaa ctggagatga ttgccggggt gttaaccagt cataaagatg
    22621 gtaaaccgtg ggtaatgcgc gtgcgttcgc aaggggagat ggattcactc gtgcgtgatg
    22681 ccggatttga taaatgcaca caacggattg atgagtgggg tatttttacg gtttcgatgg
    22741 cggtgcgtcg tgataactga acgtcggaac gtattgctac aaggcgctgg ctggttattg
    22801 ttgctggccc cgtttttctt cttcacctat ggatctctta atcagttcac cgcggttcag
    22861 gaccttaaca gccatgatat ccccagtcag gtattcggtt gggaaacggc gatccctttt
    22921 cttccctgga ctattgttcc ttactggagt ctggatcttt tatatggatt ttcgctgttc
    22981 gtttgtagca cgacattcga acagcgccga cttgtccacc ggcttattct ggcaacggta
    23041 atggcctgct gcggtttttt gctctatccg ctgaagttta gttttatccg tcctgaagtg
    23101 agtggggtga cgggatggct attttcgcaa cttgaactgt ttgatctgcc ttataaccag
    23161 tctccttcgc tgcatattat tctctgctgg ctactttggc gtcactttcg tcagcatctg
    23221 gctgagaggt ggcgtaaagt ctgtggcgga tggtttttac tcatcgctat ttctactctg
    23281 acgacctggc agcatcattt tattgatgtc atcacagggc tggcggtagg tatgttgatt
    23341 gactggatgg tgcccgtcga tcgtcgttgg aattatcaga aacctgatca acgtcgaatc
    23401 aaaatagcac tgccatatgt cgtaggcgcg ggctcgtgca ttgtgttgat ggagctaatg
    23461 gtgatgattc agttatggtg gtcagtctgg ttatgttggc cagtattatc gctactcatt
    23521 attggccgtg ggtacggtgg gcttggcgcg ataacaacag ggaaagatag tcaggggaaa
    23581 ctcccgcccg ccgtttactg gctgacattg ccctggcgca tcgggatgtg gctgtctatg
    23641 cgttggtttt gtcgtcgcct agagccggtg agcaaaatta ctgctggtgt ttatttaggg
    23701 gcgtttccac gacatattcc ggcacagaat gcggttctgg acgtcacctt tgaattccct
    23761 cggggacgag cgacaaaaga tcgactctat ttctgtgtac cgatgctgga tctggtggtt
    23821 ccggaagagg gggagctccg acaggccgtg gcgatgctgg aaacattacg cgaagagcaa
    23881 ggcagcgttc tggtccattg cgcgttggga ttatcgcgca gtgcgctggt agtggcggca
    23941 tggttgttat gttacggaca ctgtaaaacc gttgatgaag cgattagtta tattcgagcc
    24001 agacgttcgc atattgtgct taaggaagat cacaaagcga tgctgaaatt atgggaaaac
    24061 aggtaagtgg attgagatgt ggactgaact acagtccaca tcaagatcgt gtccggttat
    24121 gcagcaacaa tgctgtcgat ggccgctttc gcgtcagact gcgctttcgc tgccatttcc
    24181 ggaccgtatg cgatcccttc ggcgaagaca aatttcacat cggtaatgcc gataaagccg
    24241 aggaacgtgg acagatacgg cgtcaccagg tccgttggtc catctttgtg gatcccaccg
    24301 cggctggtaa taacgatggc ttttttaccc gttaccagac cttccggacc gttctcggta
    24361 tagcggaaag taacgcctgc gcgtgcaacc aggtcaaaat agtttttcaa ctgagttgag
    24421 atgttgaagt tatacatcgg tgccgcaata acgataacgt cgtgggcttt cagctcggca
    24481 atcagctcat cggaaagcgc cagagcttcc tgctgacgcg gagtcagcgg cgcatcgctc
    24541 ggacgcagag cgccaaccag ttcgccatcc agtaccggaa tcggatttgc agccaggtcg
    24601 cgaacggtga tttcatcagc ggagtgcttt tcgcgccatt gttcaacaaa ataatcggac
    24661 aactgattag actgagagta ccctgccagg atgctggatt taagaactaa taccttgctc
    24721 atggtgtttc cttatagatg tttgaatggg cgatgccccg ttgcttgctg acactttatt
    24781 cacaatcctg ccacagagat agcgcaataa atcgaagcct atgttcgaat ttattgaaca
    24841 acgcatagaa agccgcgatg tggtactcta tatctatcat ttaaaagaaa attaatcagg
    24901 cagactactg cccactaacg ttatgacaga acaacaaaaa ttgaccttta ctgctttgca
    24961 gcagcggctg gattcgttga tgctgcgcga cagactgcgt ttttctcgcc gtctgcatgg
    25021 tgttaagaag gttaaaaatc ctgatgcaca acaggccatt ttccaggaga tggcgaaaga
    25081 gattgaccag gcggcaggga aagtcctgct gcgtgaagcg gcacgaccgg aaattactta
    25141 tcctgacaat ttaccggtta gtcagaaaaa acaggacatt ctcgaagcga ttcgtgatca
    25201 ccaggtggtg atcgtcgccg gggaaacggg ttctggtaaa acgactcagt taccgaaaat
    25261 ctgtatggag ctggggcgcg ggattaaagg gctgatcggc catacccagc cgcgtcgcct
    25321 ggcggcaaga atggtggcga accgtattgc ggaagagctg aaaacggagc cgggcggttg
    25381 catcggttac aaagtgcgtt tcagcgatca cgtaagtgat aacacgatgg tcaagctgat
    25441 gaccgacggt atcctgctgg cggagatcca gcaagaccgc ctgctgatgc agtacgacac
    25501 catcattatt gacgaagcgc acgaacgcag cctgaatatc gattttctgc tcggctattt
    25561 gaaagagttg ctgccgcggc gtcctgacct aaaaatcatt atcacttccg cgactatcga
    25621 cccggaacgc ttttcgcgcc actttaataa tgcgccgatt atcgaagtct ccggtcggac
    25681 ctatccggtg gaagtgcgct atcgaccgat tgttgaagaa gccgatgaca ccgagcgcga
    25741 tcagttgcag gcgatttttg acgccgtaga cgaactgagt caggaaagcc ctggcgacat
    25801 tctgatcttt atgagtggtg agcgggaaat tcgcgatacc gccgatgcgc tgaacaagct
    25861 gaacttacgc cataccgaaa tcttgccgct ttatgcgcgg ctttcgaata gcgaacagaa
    25921 ccgcgtgttc cagtcgcaca gcgggcgacg cattgtgctg gcgaccaacg tcgcggaaac
    25981 ctcgctgact gtaccgggga ttaaatacgt tatcgacccc ggtacggcgc gtatcagccg
    26041 ctacagctat cgcaccaaag tgcagcgttt gccgattgaa cccatttcac aggcgtctgc
    26101 caatcagcgt aaaggccgtt gtggtcgtgt gtccgaaggg atctgtattc gtctctattc
    26161 cgaagacgat ttcctctcgc gcccggagtt taccgatccg gagattctgc gtaccaacct
    26221 ggcctcggtt attttgcaga tgaccgcgct ggggctgggc gatatcgctg cgttcccgtt
    26281 tgtcgaagca ccggataaac gcaatatcca ggatggcgtg cgtctgctcg aagaactggg
    26341 cgcgatcacc acggatgaac aggccagcgc ctataaactg acgccgctcg gtcgccagct
    26401 ctcgcaactg ccggtcgatc ctcgtctggc gcgtatggtg ctggaagcgc agaaacatgg
    26461 ctgcgtgcgt gaggcgatga ttatcacgtc cgcgctctcc attcaggacc cgcgtgagcg
    26521 gccgatggac aaacagcagg catcggacga aaaacatcgt cgcttccacg acaaagaatc
    26581 cgacttcctc gcgtttgtaa atctgtggaa ttatcttggc gagcagcaaa aggcgctttc
    26641 ttcaaacgcc ttccgtcgcc tgtgtcgtac cgattatctc aactatctgc gcgtgcgcga
    26701 atggcaggat atctacaccc agttgcgtca ggtggtgaaa gaacttggca ttccggttaa
    26761 cagcgaaccg gcggagtatc gcgaaattca cattgcgttg ctgacgggtt tactttccca
    26821 tatcggcatg aaagatgccg ataaacaaga atataccggc gcacgtaacg cgcgtttatc
    26881 catcttcccc ggttctggtt tattcaaaaa accgccgaaa tgggtaatgg tggcggaact
    26941 ggtagaaacc agccgcctgt gggggcgcat tgctgcgcgt atcgacccgg aatgggtgga
    27001 gcctgtcgct cagcatttga ttaaacgtac ctacagcgaa ccgcactggg aacgggcgca
    27061 gggcgcggtg atggcaacgg aaaaagtcac tgtttatggt ttgccgattg ttgccgcgcg
    27121 caaggtcaac tacagccaga tcgatccggc gttatgtcgt gaactcttta ttcgccacgc
    27181 tctggtggaa ggtgactggc agacgcgtca cgcattcttc cgtgaaaacc tgaaactgcg
    27241 ggccgaagtg gaagagctgg aacacaaatc acgtcgccgc gatattctgg ttgatgacga
    27301 aacgctgttt gagttctacg accagcgcat cagccacgat gtaatctccg ctcgtcactt
    27361 cgatagctgg tggaaaaaag tcagccgcga aacgcctgat ttgctcaact ttgaaaagag
    27421 catgttgatc aaagaagggg cggaaaaaat cagcaagctg gattacccga acttctggca
    27481 tcagggcaat ctcaagctgc gtttgagcta tcagtttgag ccaggcgcgg acgcggacgg
    27541 tgtgaccgta catattccgc tgccgttact taaccaggtt gaggaaagcg ggtttgaatg
    27601 gcagatcccc ggtctgcgcc gcgaattggt gattgctctg attaaatcgt tgccgaaacc
    27661 ggtacgccgt aattttgtgc ctgcaccaaa ctacgccgaa gcgtttttag gccgcgtcac
    27721 accgctggag ttaccgttgc tcgacagcct tgagcgcgag ttacggcgga tgaccggcgt
    27781 taccgttgac cgcgaagact ggcactggga tcaggtgccc gatcacctga aaatcacctt
    27841 ccgcgtggtg gatgacaaaa acaagaagct aaaagaaggg cgctcgctac aagatctgaa
    27901 agatgcgctg aaaggcaaag tgcaggaaac gctgtctgcg gtggcggatg acggtatcga
    27961 gcagagcgga ctgcatatct ggagttttgg tcagctgccg gaaagctacg aacagaagcg
    28021 tggcaactac aaagtgaagg cgtggccagc gctagtggat gaacgcgaca gtgtggcgat
    28081 caaactgttt gataatccgc tggagcaaaa acaggcaatg tggaacggtc ttcgccgtct
    28141 actgctgctg aatattccct cgccgatcaa gtatttacat gagaagttac caaacaaagc
    28201 caagctgggg ctgtacttta acccgtatgg caaagtgctg gagctgatcg acgactgtat
    28261 ctcctgcggt gtggataaat tgatcgacgc caatggtggc ccggtctgga cggaagaagg
    28321 ctttgctgcg ctgcatgaaa aagtgcgtgc cgaactgaac gacacggtgg tggatattgc
    28381 gaagcaggtc gagcaaatcc ttacggcagt gttcaatatc aacaaacgtc tgaaggggcg
    28441 ggtggatatg acgatggcgc tggggctttc tgacattaaa gcgcagatgg gcgggctggt
    28501 atatcgcggt tttgtcactg gtaacggctt caaacggctg ggcgacacgc tgcgatattt
    28561 gcaggcgatt gaaaaacggc tggaaaaact ggcggttgat ccacatcgcg accgtgctca
    28621 gatgctgaaa gtcgaaaacg tccagcaggc gtggcagcaa tggatcaaca aactgccgcc
    28681 tgcacgtcgt gaggatgaag acgtgaaaga gatccgttgg atgatagaag agttgcgcgt
    28741 tagttacttc gctcaacaac ttggtacgcc ttatccgatt tcagataagc gtattttgca
    28801 ggcgatggag cagattagcg gttaaccctg ctatttgcct gataaagaaa aaacccggta
    28861 agcatttagc gccgggtttt ttattaattc taaaacggta agggtaaaaa ttcaggaatt
    28921 cagaaaaata caattctctg ctgcaagatg aataatgttt atctacagca tttccttaaa
    28981 agatatgtca ggcttgcgga gtggcggtta aggaaatacg acttcctcct ttcagagtgc
    29041 tccgcttctc actattatct cacgcagtat tcttaaggga acgataagga ggaaccatga
    29101 acattacccc gtttccgacg ctttcgccgg caactataga tgccataaat gttatcggac
    29161 agtggctggc gcaggatgat ttctccggtg aggtgccgta tcaggccgat tgcgtgatcc
    29221 ttgcaggcaa tgcggttatg ccgactatcg acgcggcatg taagattgct cgcgatcagc
    29281 aaattccttt actgattagt ggtggtatcg gtcactcgac aacttttttg tatagcgcca
    29341 tcgcacagca tccgcactac aacactatcc gcaccactgg cagagcagaa gcgaccatcc
    29401 tggcggatat cgctcatcag ttctggcaca ttccgcatga aaaaatctgg attgaagacc
    29461 agtcaacaaa ctgcggtgaa aacgcacgct ttagcatcgt gctattgaat caggccgtag
    29521 aacgagttca tacggctatc gttgttcagg accccaccat gcagcggcgc acgatggcga
    29581 cgttccgccg tatgactggg gacaatcccg atgcaccacg ctggttaagt tatcccggat
    29641 tcgttcctca gttaggaaat aacgcagaca gtgtaatctt tattaatcag ttacaaggat
    29701 tatggccggt tgagcgttat ctctcactac tcactggcga gctgccgcgt ttacgcgatg
    29761 atagcgatgg ctacggtccc cgcgggcgag attttatcgt tcacgttgat tttccggcag
    29821 aagtcatcca tgcatggcaa acgcttaaac acgatgcggt gctcatcgag gcgatggaaa
    29881 gtcgctcgtt acgttaaaaa ttgcccgttt gtgaaccact tgtttgcaaa cgggcatgac
    29941 tcctgacttt tatttctgcc ttttattcct tttacacttg tttttatgaa gcccttcaca
    30001 gaattgtcct ttcacgatgc cgtctctctg atgattgatg ttaattaaca atgcattcac
    30061 cgaaaacaaa catataaatc acaggagtcg cccatgtcag tacccgttca acatcctatg
    30121 tatatcgatg gacagtttgt tacctggcgt ggagacgcat ggattgatgt ggtaaaccct
    30181 gctacagagg ctgtcatttc ccgcataccc gatggtcagg ccgaggatgc ccgtaaggca
    30241 atcgatgcag cagaacgtgc acaaccagaa tgggaagcgt tgcctgctat tgaacgcgcc
    30301 agttggttgc gcaaaatctc cgccgggatc cgcgaacgcg ccagtgaaat cagtgcgctg
    30361 attgttgaag aagggggcaa gatccagcag ctggctgaag tcgaagtggc ttttactgcc
    30421 gactatatcg attacatggc ggagtgggca cggcgttacg agggcgagat tattcaaagc
    30481 gatcgtccag gagaaaatat tcttttgttt aaacgtgcgc ttggtgtgac taccggcatt
    30541 ctgccgtgga acttcccgtt cttcctcatt gcccgcaaaa tggctcccgc tcttttgacc
    30601 ggtaatacca tcgtcattaa acctagtgaa tttacgccaa acaatgcgat tgcattcgcc
    30661 aaaatcgtcg atgaaatagg ccttccgcgc ggcgtgttta accttgtact ggggcgtggt
    30721 gaaaccgttg ggcaagaact ggcgggtaac ccaaaggtcg caatggtcag tatgacaggc
    30781 agcgtctctg caggtgagaa gatcatggcg actgcggcga aaaacatcac caaagtgtgt
    30841 ctggaattgg ggggtaaagc accagctatc gtaatggacg atgccgatct tgaactggca
    30901 gtcaaagcca tcgttgattc acgcgtcatt aatagtgggc aagtgtgtaa ctgtgcagaa
    30961 cgtgtttatg tacagaaagg catttatgat cagttcgtca atcggctggg tgaagcgatg
    31021 caggcggttc aatttggtaa ccccgctgaa cgcaacgaca ttgcgatggg gccattgatt
    31081 aacgccgcgg cgctggaaag ggttgagcaa aaagtggcgc gcgcagtaga agaaggggcg
    31141 agagtggcgt tgggtggcaa agcggtagag tggaaaggat attattatcc gccgacattg
    31201 ctgctggatg ttcgccagga aatgtcgatt atgcatgagg aaacctttgg cccggttctg
    31261 ccggtggtcg catttgacac gctggaagag gctatctcaa tggctaatga cagtgattac
    31321 ggcctgacct catcaatcta tacccaaaat ctgaacgtcg cgatgaaagc cattaaaggg
    31381 ctgaagtttg gtgaaactta catcaaccgt gaaaacttcg aagctatgca aggcttccac
    31441 gccggatggc gtaaatccgg tattggcggc gcagatggta aacatggcct gcatgaatat
    31501 ctgcagaccc aggtggttta tttacagtcg taatgagtaa aagaggcgga ggttttttcc
    31561 tccgcctgtg cgcgtcagag tttagcgaat ttttcgaggg tgcgaataag ctgcgtgacg
    31621 aagccatatt cgttatcgta ccaggcgacc gttttcacca gttgtaaatc gcccacggcg
    31681 gtaatttccg tttgcgtggc atcaaacacc gaaccgaaat ggctgccaat gatatcggaa
    31741 gagactattt cttcatcggt ataaccaaat gactcgttat tggtggttgc ttgtttaagt
    31801 gcgttattca cctcttcggc agtcactttt tttccgagaa tcaataccag ttcagtgacc
    31861 gaacctgttt tcaccggcac gcgttgcgca tgacctttca gtttgccgct cagttccggg
    31921 atcaccagac caatggcttt tgccgccccc gtagtgtggg gaatgatatt ttctgccgct
    31981 gcgcgtgaag cacgtaaatc tttaccacgt gggccatcca ccagtgactg ggtgccagta
    32041 taggcatgaa tggtcgtcat cgtgccgact tctatcccga aactgtcatg caaggctttg
    32101 gccatcggcg caagacagtt agtggtgcat gacgccacgg aaacaatggt gtcgttgcca
    32161 tccagagtgt cgtcattgac gttataaacg atagttttca tttcaccggc aggggcggaa
    32221 atcaacacct tcttcgcacc agcatcaaga tgcgcctgcg atttctcggc ggaggtataa
    32281 aagccagtac attcgacaat gatttctgca cctttcgctt tccacggaat atttttagcc
    32341 tctttttcgg cgtaaaccgc gatacttttc ccatcaacga taagtgaatc ttccgtaaaa
    32401 tcaacgctcc aggggaatgg tccgtagttt gaatcatgtt tcagcaggta ggcgagaatt
    32461 tttggggaag tgagatcatt aatagcgaca acgtctatgt tgcttttgac ttcaagtaat
    32521 cgacgcaaca ccagtcgacc gatacgacca aaaccgttaa taccaacttt actcatggtt
    32581 ttctcctgtc aggaacattc ggatgaaaat tgatcctttc caggcttaga ccaggattgt
    32641 gggatgggca atctccattc ttacagtgaa acgtaacgta ctgaaaacgg gtgaacaata
    32701 tttaatgaaa ttttgagaaa agcccgttat gttaacggaa aattatgtta aagcaggaaa
    32761 tgttatggaa aataaatact caaggttaca aatcagcatt cactggctgg tctttttgct
    32821 ggttatcgca gcgtattgcg caatggagtt tcgtggtttc ttcccacgta gcgatcggcc
    32881 actcatcaac atgattcatg tttcctgcgg catctcaatt ctcgtgctga tggtcgttcg
    32941 tcttttgtta aggctgaaat acccaacccc gccgattata cctaagccaa aaccgatgat
    33001 gacgggactg gcgcatttgg gacatttggt gatttatctc ctgtttattg cgctgccagt
    33061 gattggtttg gtgatgatgt ataaccgggg caacccgtgg tttgcgtttg gtttgacgat
    33121 gccttacgct tcagaggcca atttcgaacg ggttgatagc ttaaagtcgt ggcatgaaac
    33181 gctggcgaat ctgggatatt ttgtcatcgg gttgcacgct gcggctgcac tggcacacca
    33241 ctatttctgg aaggacaaca cacttctacg catgatgccg cgtaaacgtt cctgaaggat
    33301 atttaaagaa aacgcctgta ctaaaaccga cccgtggtac aggcgaagaa tacgggtcta
    33361 catcggaagc gcctattata tttatttgta tgacaaataa aaccataatc cttgcccata
    33421 cgtccatctg gcttattttt aatcaattca cccgatcttt gatctcatca acggtatcaa
    33481 aataaaccag cgtataacgt tcaaatcgaa cataagattc gataaaccat ggaggttata
    33541 tgaaaaaact ggcacttatt ttgtttatgg gaacgcttgt ttccttttat gccgatgccg
    33601 ggcgcaaacc ctgttctggt tcgaaagggg ggatctcaca ctgtacggca ggcggcaaat
    33661 ttgtctgtaa tgatggttct attagtgcat cgaaaaaaac atgcactaac tgaagtgtaa
    33721 aaggggtgcc atgagaaaat gaatccgcta gtcagatgcg cggggaaaat ccccgcgctt
    33781 gcccttacct ggacgtgcag gccatgagcg cagccacctc cttatcaccg tcccggaacc
    33841 gcagttcgta gagcgtttgt cgggtcagga gtgtgaatgt cagaatcgta atgcagataa
    33901 tgagcagaca cactaccaga gggttgtgct tcatagcctt tctccttgcc ggatggcggg
    33961 taagaggcta agatctgaat tgctaggttc attcgttggc ctcgg
//
LOCUS       DAZKHC020000046        33949 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZKHC020000046 DAZKHC020000000
VERSION     DAZKHC020000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 33949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.046"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            163..1221
                     /gene="citC"
                     /locus_tag="ACHOSM_RS12515"
                     /old_locus_tag="pgaptmp_002503"
     CDS             163..1221
                     /gene="citC"
                     /locus_tag="ACHOSM_RS12515"
                     /old_locus_tag="pgaptmp_002503"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HGU4559000.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1236..1532
                     /gene="citD"
                     /locus_tag="ACHOSM_RS12520"
                     /old_locus_tag="pgaptmp_002504"
     CDS             1236..1532
                     /gene="citD"
                     /locus_tag="ACHOSM_RS12520"
                     /old_locus_tag="pgaptmp_002504"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HGU4559001.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1529..2437
                     /gene="citE"
                     /locus_tag="ACHOSM_RS12525"
                     /old_locus_tag="pgaptmp_002505"
     CDS             1529..2437
                     /gene="citE"
                     /locus_tag="ACHOSM_RS12525"
                     /old_locus_tag="pgaptmp_002505"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HGU4559002.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2448..3980
                     /gene="citF"
                     /locus_tag="ACHOSM_RS12530"
                     /old_locus_tag="pgaptmp_002506"
     CDS             2448..3980
                     /gene="citF"
                     /locus_tag="ACHOSM_RS12530"
                     /old_locus_tag="pgaptmp_002506"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HGU4559003.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRHSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3984..4535
                     /gene="citX"
                     /locus_tag="ACHOSM_RS12535"
                     /old_locus_tag="pgaptmp_002507"
     CDS             3984..4535
                     /gene="citX"
                     /locus_tag="ACHOSM_RS12535"
                     /old_locus_tag="pgaptmp_002507"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HGU4559004.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRSCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4510..5388
                     /gene="citG"
                     /locus_tag="ACHOSM_RS12540"
                     /old_locus_tag="pgaptmp_002508"
     CDS             4510..5388
                     /gene="citG"
                     /locus_tag="ACHOSM_RS12540"
                     /old_locus_tag="pgaptmp_002508"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HGU4559005.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5439..6902
                     /gene="citT"
                     /locus_tag="ACHOSM_RS12545"
                     /old_locus_tag="pgaptmp_002509"
     CDS             5439..6902
                     /gene="citT"
                     /locus_tag="ACHOSM_RS12545"
                     /old_locus_tag="pgaptmp_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HGU4559006.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7016..7822
                     /gene="rna"
                     /locus_tag="ACHOSM_RS12550"
                     /old_locus_tag="pgaptmp_002510"
     CDS             7016..7822
                     /gene="rna"
                     /locus_tag="ACHOSM_RS12550"
                     /old_locus_tag="pgaptmp_002510"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HGU4559007.1"
                     /translation="MKAFWRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8052..8462
                     /gene="rnk"
                     /locus_tag="ACHOSM_RS12555"
                     /old_locus_tag="pgaptmp_002511"
     CDS             8052..8462
                     /gene="rnk"
                     /locus_tag="ACHOSM_RS12555"
                     /old_locus_tag="pgaptmp_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HGU4559008.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8600..8689
                     /gene="yldA"
                     /locus_tag="ACHOSM_RS12560"
                     /old_locus_tag="pgaptmp_002512"
     CDS             8600..8689
                     /gene="yldA"
                     /locus_tag="ACHOSM_RS12560"
                     /old_locus_tag="pgaptmp_002512"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HGU4559009.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8693..9931)
                     /gene="ybdR"
                     /locus_tag="ACHOSM_RS12565"
                     /old_locus_tag="pgaptmp_002513"
     CDS             complement(8693..9931)
                     /gene="ybdR"
                     /locus_tag="ACHOSM_RS12565"
                     /old_locus_tag="pgaptmp_002513"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HGU4559010.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILLTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10152..10580
                     /gene="uspG"
                     /locus_tag="ACHOSM_RS12570"
                     /old_locus_tag="pgaptmp_002514"
     CDS             10152..10580
                     /gene="uspG"
                     /locus_tag="ACHOSM_RS12570"
                     /old_locus_tag="pgaptmp_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HGU4559011.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10701..12266)
                     /gene="ahpF"
                     /locus_tag="ACHOSM_RS12575"
                     /old_locus_tag="pgaptmp_002515"
     CDS             complement(10701..12266)
                     /gene="ahpF"
                     /locus_tag="ACHOSM_RS12575"
                     /old_locus_tag="pgaptmp_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HGU4559012.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12437..13000)
                     /gene="ahpC"
                     /locus_tag="ACHOSM_RS12580"
                     /old_locus_tag="pgaptmp_002516"
     CDS             complement(12437..13000)
                     /gene="ahpC"
                     /locus_tag="ACHOSM_RS12580"
                     /old_locus_tag="pgaptmp_002516"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HGU4559013.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13372..14118
                     /gene="dsbG"
                     /locus_tag="ACHOSM_RS12585"
                     /old_locus_tag="pgaptmp_002517"
     CDS             13372..14118
                     /gene="dsbG"
                     /locus_tag="ACHOSM_RS12585"
                     /old_locus_tag="pgaptmp_002517"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HGU4559014.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14328..15230
                     /gene="ybdO"
                     /locus_tag="ACHOSM_RS12590"
                     /old_locus_tag="pgaptmp_002518"
     CDS             14328..15230
                     /gene="ybdO"
                     /locus_tag="ACHOSM_RS12590"
                     /old_locus_tag="pgaptmp_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4559015.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHNILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15377..16597
                     /gene="ybdN"
                     /locus_tag="ACHOSM_RS12595"
                     /old_locus_tag="pgaptmp_002519"
     CDS             15377..16597
                     /gene="ybdN"
                     /locus_tag="ACHOSM_RS12595"
                     /old_locus_tag="pgaptmp_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU4559016.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPCVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            16570..17199
                     /gene="ybdM"
                     /locus_tag="ACHOSM_RS12600"
                     /old_locus_tag="pgaptmp_002520"
     CDS             16570..17199
                     /gene="ybdM"
                     /locus_tag="ACHOSM_RS12600"
                     /old_locus_tag="pgaptmp_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGU4559017.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNGAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(17200..18360)
                     /gene="ybdL"
                     /locus_tag="ACHOSM_RS12605"
                     /old_locus_tag="pgaptmp_002521"
     CDS             complement(17200..18360)
                     /gene="ybdL"
                     /locus_tag="ACHOSM_RS12605"
                     /old_locus_tag="pgaptmp_002521"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HGU4559018.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVHALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSSLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            18469..19557
                     /gene="hcxA"
                     /locus_tag="ACHOSM_RS12610"
                     /old_locus_tag="pgaptmp_002522"
     CDS             18469..19557
                     /gene="hcxA"
                     /locus_tag="ACHOSM_RS12610"
                     /old_locus_tag="pgaptmp_002522"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HGU4559019.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGS
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELDVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(19567..19764)
                     /gene="ybdD"
                     /locus_tag="ACHOSM_RS12615"
                     /old_locus_tag="pgaptmp_002523"
     CDS             complement(19567..19764)
                     /gene="ybdD"
                     /locus_tag="ACHOSM_RS12615"
                     /old_locus_tag="pgaptmp_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU4559020.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(19940..22045)
                     /gene="cstA"
                     /locus_tag="ACHOSM_RS12620"
                     /old_locus_tag="pgaptmp_002524"
     CDS             complement(19940..22045)
                     /gene="cstA"
                     /locus_tag="ACHOSM_RS12620"
                     /old_locus_tag="pgaptmp_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HGU4559021.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVLTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(22226..22639)
                     /gene="entH"
                     /locus_tag="ACHOSM_RS12625"
                     /old_locus_tag="pgaptmp_002525"
     CDS             complement(22226..22639)
                     /gene="entH"
                     /locus_tag="ACHOSM_RS12625"
                     /old_locus_tag="pgaptmp_002525"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HGU4559022.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(22642..23388)
                     /gene="entA"
                     /locus_tag="ACHOSM_RS12630"
                     /old_locus_tag="pgaptmp_002526"
     CDS             complement(22642..23388)
                     /gene="entA"
                     /locus_tag="ACHOSM_RS12630"
                     /old_locus_tag="pgaptmp_002526"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HGU4559023.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(23388..24245)
                     /gene="entB"
                     /locus_tag="ACHOSM_RS12635"
                     /old_locus_tag="pgaptmp_002527"
     CDS             complement(23388..24245)
                     /gene="entB"
                     /locus_tag="ACHOSM_RS12635"
                     /old_locus_tag="pgaptmp_002527"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HGU4559024.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFNRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(24259..25869)
                     /gene="entE"
                     /locus_tag="ACHOSM_RS12640"
                     /old_locus_tag="pgaptmp_002528"
     CDS             complement(24259..25869)
                     /gene="entE"
                     /locus_tag="ACHOSM_RS12640"
                     /old_locus_tag="pgaptmp_002528"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HGU4559025.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLYTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGALGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCLLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(25879..27054)
                     /gene="entC"
                     /locus_tag="ACHOSM_RS12645"
                     /old_locus_tag="pgaptmp_002529"
     CDS             complement(25879..27054)
                     /gene="entC"
                     /locus_tag="ACHOSM_RS12645"
                     /old_locus_tag="pgaptmp_002529"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HGU4559026.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            27429..28394
                     /gene="fepB"
                     /locus_tag="ACHOSM_RS12650"
                     /old_locus_tag="pgaptmp_002530"
     CDS             27429..28394
                     /gene="fepB"
                     /locus_tag="ACHOSM_RS12650"
                     /old_locus_tag="pgaptmp_002530"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU4559027.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLDRLNSLFKKD"
     gene            complement(28475..29725)
                     /gene="entS"
                     /locus_tag="ACHOSM_RS12655"
                     /old_locus_tag="pgaptmp_002531"
     CDS             complement(28475..29725)
                     /gene="entS"
                     /locus_tag="ACHOSM_RS12655"
                     /old_locus_tag="pgaptmp_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HGU4559028.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            29836..30840
                     /gene="fepD"
                     /locus_tag="ACHOSM_RS12660"
                     /old_locus_tag="pgaptmp_002532"
     CDS             29836..30840
                     /gene="fepD"
                     /locus_tag="ACHOSM_RS12660"
                     /old_locus_tag="pgaptmp_002532"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HGU4559029.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTAAALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            30837..31829
                     /gene="fepG"
                     /locus_tag="ACHOSM_RS12665"
                     /old_locus_tag="pgaptmp_002533"
     CDS             30837..31829
                     /gene="fepG"
                     /locus_tag="ACHOSM_RS12665"
                     /old_locus_tag="pgaptmp_002533"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HGU4559030.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     SDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHISRRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            31826..32641
                     /gene="fepC"
                     /locus_tag="ACHOSM_RS12670"
                     /old_locus_tag="pgaptmp_002534"
     CDS             31826..32641
                     /gene="fepC"
                     /locus_tag="ACHOSM_RS12670"
                     /old_locus_tag="pgaptmp_002534"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU4559031.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(32638..33771)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOSM_RS12675"
                     /old_locus_tag="pgaptmp_002535"
     CDS             complement(32638..33771)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOSM_RS12675"
                     /old_locus_tag="pgaptmp_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HGU4559032.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMAARKQDAMMADHLV"
BASE COUNT         8145 a         9262 c         8559 g         7983 t
ORIGIN      
        1 tcacgccgaa atactcctta ggatgtatag cgaaaggaga aaaagatata cctcgatcac
       61 ccccctttct cccaagtgaa aataaaaggt tatcagtttg caacattgaa caacattcgt
      121 tgcaaatcga taacaacatg caccttcagg atactattta ttatgttcgg caatgatatt
      181 ttcacccgcg taaaacgttc agaaaataaa aaaatggcgg aaatcgccca attcctgcat
      241 gaaaatgatt tgagcgttga caccacagtc gaagtattta ttaccgtaac ccgcgatgaa
      301 aagcttatcg cgtgcggtgg aattgccgga aatattatta aatgcgttgc tatcagtgaa
      361 tccgttcgcg gtgaaggact ggcgctgaca ttagccactg aactgataaa cctcgcctat
      421 gagcggcaca gcacgcatct gtttatttat accaaaaccg aatacgaggc gctgttccgc
      481 cagtgcggtt tttccacgct gaccagcgta cccggcgtga tggtgctgat ggaaaacagc
      541 gccacgcgac tgaaacgcta tgccgaatcg ctgaaaaaat ttcgtcatcc agggaacaag
      601 attggctgca ttgtgatgaa cgccaatccc tttacgaatg gtcaccgtta tctgattcaa
      661 caagctgcgg cacagtgcga ctggttgcat ctgtttttag tcaaagaaga ttcttcacgc
      721 ttcccctatg aagaccggct ggatctggtg ttaaaaggca ccgccgatat tccacgcctg
      781 actgtgcatc gcggctccga atacatcatc tcccgcgcta cgttcccttg ctacttcatt
      841 aaagaacaga gcgtcattaa ccattgttac accgaaattg atctgaagat tttccgtcag
      901 tacctcgctc ccgcactggg tgtaactcac cgctttgtcg gtactgaacc cttttgtcgc
      961 gttaccgccc agtacaacca ggatatgcgc tactggctgg aaacgccgac tatctccgca
     1021 ccgcccatcg aactggttga aattgagcgg ctgcgttacc aggagatgcc gatatccgct
     1081 tcccgggtac gtcaactgct ggcgaaaaac gatctcacgg ctatcgcgcc gctggtccct
     1141 gcagtcacgc tgcattattt gcagaacctg cttgagcact cccgccagga cgcggcagct
     1201 cgtcaaaaga cccccgcatg agaaacaggt gaaaaatgaa aataaaccag cccgccgttg
     1261 caggcaccct tgagtctggg gatgtgatga tacgcatcgc cccactcgat acgcaggata
     1321 tcgacctgca aatcaacagc agcgttgaga aacagtttgg cgatgcaatt cgcaccacca
     1381 ttctggacgt tctcgcccgc tacaacgtgc gcggcgtaca gctgaatgtc gatgacaaag
     1441 gcgcactgga ctgcatttta cgtgcccgac tggaagccct gctggcacgc gccagcggta
     1501 tcccggctct gccatgggag gattgccaat gatttccgct tcgctgcaac aacgtaaaac
     1561 tcgcacccgc cgcagcatgt tgtttgtgcc tggtgccaat gccgcgatgg tcagcaactc
     1621 cttcatttac ccggctgatg ccctgatgtt tgacctcgaa gactccgtgg cattacgtga
     1681 aaaagacacc gcccgccgca tggtttatca cgcgctgcaa catccgctgt atcgcgatat
     1741 tgaaaccatt gtgcgtgtca acgcgctgga ttccgaatgg ggtgttaacg acctggaagc
     1801 cgtcgttcgc ggtggtgcgg acgttgtgcg tctgccgaaa accgataccg ctcaggatgt
     1861 tctggatatt gaaaaagaga tcctgcgtat cgaaaaagcc tgtggtcgtg aacccggcag
     1921 caccggcctg ctggcggcga ttgaatctcc gctgggcatt acccgcgcag tggaaatcgc
     1981 tcacgcttcc gagcgtttga tcggtatcgc cctcggtgca gaagactatg tgcgcaacct
     2041 gcgtacagaa cgctccccgg aaggaactga actgctgttc gcacgctgtt ccattttgca
     2101 ggccgcgcgc tctgcgggta ttcaggcgtt cgataccgtc tattccgacg ctaacaacga
     2161 agccggattc ctgcaagaag ccgcccacat caaacagctg ggctttgacg gcaaatcgct
     2221 gatcaacccg cgtcagattg atctgctgca caacctctac gcaccgaccc agaaagaagt
     2281 ggatcacgcc cgccgcgtcg tagaagccgc tgaagccgcc gctcgcgaag gcctcggcgt
     2341 ggtttccctg aacggcaaga tggtggacgg tccggttatc gatcgcgccc gtctggtgct
     2401 ctcccgtgca gaactttccg gcatccgcga agaataaggc aatcaaaatg acgcagaaaa
     2461 ttgaacaatc tcaacgacaa gaacgggtag cggcctggaa tcgtcgcgct gaatgcgatc
     2521 ttgccgcttt ccagaactca ccaaagcaaa cctaccaggc tgaaaaagcg cgcgatcgca
     2581 aactgtgcgc caacctggaa gaagcgattc gtcactctgg tttacaggac ggcatgacgg
     2641 tttccttcca ccacgctttc cgtggcggtg acctgaccgt caatatggtg atggacgtca
     2701 tcgcgaagat gggctttaaa aacctgactc tggcctccag ctccctgagt gattgccatg
     2761 cgccgctggt agaacacatt cgccagggcg tggttacccg catttatacc tctggcctgc
     2821 gcggtccact ggcggaagaa atctcccgtg gtctgctggc ggaaccggtg caaatccact
     2881 ctcacggcgg acgtgtgcat ctggtacaga gcggcgaact gaatatcgac gtggctttcc
     2941 tcggcgtccc gtcctgtgat gaattcggta atgccaacgg ctacaccggt aaagcctgct
     3001 gtggctccct cggctatgca atggttgatg ccgacaacgc aaaacaggtc gtgatgctta
     3061 ccgaagaact gctgccttat ccgcataatc cggcaagcat tgagcaagat caggttgatt
     3121 tgatcgtcaa agttgatcgc gttggcgatg ctgcaaaaat cggcgctggt gcgacccgta
     3181 tgaccactaa cccgcgcgaa ctgcttattg cccgtagcgc tgcggatgtg attgtcaact
     3241 ctggctactt caaagaaggt ttctccatgc aaaccggcac cggcggcgct tcgctggcgg
     3301 taacccgttt cctggaagac aaaatgcgta gccgcgatat tcgcgccgac ttcgccctcg
     3361 gcggtattac tgcaacgatg gttgatctgc acgaaaaagg tctgatccgc aaactgctgg
     3421 atgtgcagag ctttgacagc cacgctgcgc aatcgctggc ccgtaacccc aatcacatcg
     3481 aaatcagcgc caaccagtac gctaactggg gttcgaaagg tgcatcggtt gatcgtctcg
     3541 acgtggtggt actgagcgcg ctggaaattg acacccagtt caacgttaac gtgctgaccg
     3601 gctctgacgg cgtactgcgt ggtgcttccg gtggtcactg cgataccgcg attgcctctg
     3661 cgctttccat catcgtcgcg ccgctggtac gcggtcgtat tccgactctg gtggataacg
     3721 tgctgacctg catcaccccg ggctccagtg tcgatattct ggtcacagac cacggtatcg
     3781 cagttaaccc ggcacgtccg gaactggcag aacgtctgca ggaagcgggc attaaagtgg
     3841 tttccattga gtggctgcgc gaacgtgcgc gtctgctgac cggtgaacca cagccgattg
     3901 aattcaccga ccgcgtcgtt gccgttgtgc gttaccgcga tggctcggtg atcgatgttg
     3961 tgcatcaggt gaaggaataa gccatgcacc tgcttcctga actcgccagc caccatgcgg
     4021 tgtcaattcc cgagctgctc gtcagccggg atgaaaggca agcacggcaa cacgtctggc
     4081 tcaagcgcca tcctgttcca ctggtctcct ttaccgtggt tgcgcctggg ccgattaaag
     4141 acagcgaggt cacacgccga atttttaatc atggcgtgac agccttgcgt gccttagccg
     4201 caaaacaggg ctggcaaatt caggagcagg ctgcactggt ttccgccagc gggccggagg
     4261 gcatgttgag cattgccgcc ccggctcgcg acctcaagct cgccaccatt gagcttgaac
     4321 atagtcatcc tctcgggcgg ttatgggata tcgatgtcct gacgcccgaa ggcgaaattc
     4381 tctcccgccg cgactattca ctgccgcctc gcagctgcct gttgtgcgag caaagcgcag
     4441 ccgtctgcgc gcgtggaaaa acccatcaac tgaccgattt actcaaccgc atggaggcac
     4501 tgctgaacga tgtcgatgcc tgcaacgtca actaaaacca caaagcttgc gacgtcatta
     4561 atcgatgagt acgccctgct gggctggcgc gccatgctga ctgaagtcaa tctgtcaccg
     4621 aaaccaggcc tcgtggatcg gattaactgc ggtgcgcata aagatatggc gctggaagat
     4681 ttccaccgca gcgcgctggc gattcagggc tggctacccc gtttcattga atttggtgcc
     4741 tgtagtgcgg aaatggcacc agaagcggta ctccacggat tacgcccaat tggtatggct
     4801 tgcgaaggtg atatgttccg cgccactgcg ggcgtaaaca cgcataaagg cagcattttt
     4861 tctttagggc tgctatgtgc ggcaattggc cgtttgcttc aactcaacca accggtaacg
     4921 ccaacaaccg tttgttctac ggcggcaagt ttctgccgtg gcctgaccga tcgagaactg
     4981 cgtaccaata attcacaact gacggcaggt caacggttgt accagcagct tggccttacc
     5041 ggcgcacgcg gtgaagccga agcgggttat ccactggtga tcaatcacgc cttgccgcat
     5101 tacctcactc tgctggatca ggggttagat cctgaactgg cattgctcga taccttgctc
     5161 ctactgatgg cgatcaacgg cgataccaac gttgcatcgc gcggtggcga ggggggcctg
     5221 cgctggctac agcgcgaggc gcaaacatta ttgcaaaaag ggggcattcg aacccccgcc
     5281 gatctcgatt atctccggca gttcgacagg gagtgtatcg aacgaaatct cagtccaggc
     5341 ggcagtgccg acctgctgat ccttacctgg tttttagcac agatttaatt atttaagcac
     5401 ttgataaatt tggaaatatc aattttcgga gaacccgtat gtctttagca aaagataata
     5461 tatggaaact attggcccca ctggtggtga tgggtgtcat gtttcttatc cctgtccccg
     5521 acggtatgcc accacaggca tggcattact tcgctgtgtt tgtggcaatg attgtcggca
     5581 tgatcctcga gccaattccg gcaacagcga tcagttttat tgcggttact atttgcgtta
     5641 ttggcagtaa ttacctgctc tttgatgcca aagaattagc tgacccagcg tttaatgcgc
     5701 aaaaacaggc gctgaaatgg ggtctggctg gtttttccag caccacggta tggctggtat
     5761 ttggcgcatt tatttttgca ttagggtatg aagtttccgg gttaggtcgt cgcattgccc
     5821 ttttcctggt gaaattcatg ggcaaacgca cgctgacgtt gggttatgcg attgtcatta
     5881 tcgacattct gctggcaccg tttacaccgt ccaacaccgc gcgtaccggg ggtacggttt
     5941 ttccggtcat taaaaacctg ccgccgctgt ttaaatcatt ccccaacgat ccgtccgcgc
     6001 gtcgtattgg cggctatttg atgtggatga tggtcattag taccagtctg agttcgtcca
     6061 tgtttgtcac cggtgcggca ccaaacgtgc tgggtctgga gttcgtcagc aaaattgccg
     6121 gtatccagat tagctggttg cagtggttcc tctgcttcct gccggttggg gttatcttgc
     6181 ttatcattgc gccgtggctt tcctacgtac tgtacaaacc ggaaatcaca cacagtgaag
     6241 aagtggcaac ctgggcgggt gatgaactga aaaccatggg tgcgctgaca cgcagagagt
     6301 ggacactgat tggccttgta ttgctcagct taggtttgtg ggtttttggc agtgaagtca
     6361 ttaatgctac tgcagttggt ctgctggcag tttcgctaat gctggccctg cacgttgtgc
     6421 cttggaaaga tattacccgc tataacagcg catggaacac gctggtcaac ctggcaactc
     6481 tggttgtgat ggctaacggc ctgactcgtt ctggttttat tgactggttc gccggcacca
     6541 tgagtacgca cctggaagga ttctcaccaa acgcaacggt gattgtactg gttctggtgt
     6601 tctactttgc acactacctg tttgccagcc tgtctgcgca caccgcaacc atgctgccgg
     6661 ttattctggc cgttggtaaa ggtattccgg gcgtaccaat ggaacaactg tgtatcctgc
     6721 tggtgctgtc tatcggtatc atgggctgtc tgacgccgta tgcaaccggt cctggggtga
     6781 ttatttacgg ctgtggctat gtgaaatcaa aagattactg gcgtcttggc gcaatcttcg
     6841 gggtgattta catctctatg ttgctgttgg ttggctggcc gattctcgcc atgtggaact
     6901 aatacgttcg ccaaacgctg aataaatcat cacgcccgcc aggtaagccc acctggcggg
     6961 cttttttatg atttaataga tagtattcag tgtatgtatg agttccacac ccattatgaa
     7021 agcattctgg cgtaacgccg cgttgctcgc ggtttctctg cttcccttat cttctgccaa
     7081 cgccgtagcg ttgcaggcaa aacagtatgg cgattttgat cgctatgtcc tggccctctc
     7141 ctggcaaacc ggattttgcc agagtcaaca cgatcgaaat cgtaacgaac gagatgaatg
     7201 tcgcctgcaa accgaaacga ccaacaaagc tgattttctg accgtacatg gtctgtggcc
     7261 aggattgcct aaatcggttg ctgcccgtgg tgttgatgaa cgccgctgga tgcgcttcgg
     7321 ttgcgctact cgcccaatcc cgaatctacc agaagcgcac gccagccgga tgtgttcatc
     7381 gccggaaacc gggttatcac tggaaacggc cgctaaacta agtgaagtca tgccaggagc
     7441 tggcggacgt tcctgcctgg aacgctacga atatgccaaa cacggtgcct gctttggttt
     7501 tgatccggac gcatacttcg gtacgatggt acgcctgaat caagaaatta aagagagcga
     7561 agccggaaaa ttccttgcgg ataattacgg taaaacagtg agccgccgtg actttgacgc
     7621 cgcctttgcc aaaagctggg gaaaagagaa cgtgaaagca gttaagctaa cgtgccaggg
     7681 taaccctgcg tatttgactg aaattcagat ctcgatcaaa gctgacgcca tcaacgctcc
     7741 gctttctgca aactcattct tgccacaacc acacccaggc aactgtggca aaacctttgt
     7801 gattgataaa gtgggttatt aatcatctga tttacaaaac acgctaattc gtcttccata
     7861 aaccgtgacg attaagaatt atacataatg cgctgcattg aggaattccc tgaatgtgac
     7921 gcaaatcact tcaaagagtg agcaacagtc agtatcatca tgtgagttaa accctcgccg
     7981 cctgacggtg agggttttct tttgggtatg tttatctgcg acactcgcag taccgacgac
     8041 atggagtaaa aatgtccaga ccaactatca tcattaacga cctggatgcc gaacgcatcg
     8101 atattctgct ggagcaaccc gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag
     8161 agttggatcg cgcccaaatg tgttcgccag aagagatgcc acacgacgtg gtgacaatga
     8221 acagccgggt taaattccgc aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt
     8281 atccggcaaa aatgaccgat agcaatactc agctttccgt tatggctccg gtaggtgccg
     8341 cactgctggg gctgcgcgtt ggcgattcca ttcactggga acttccgggc ggcgttgcaa
     8401 cccaccttga agtgctggaa ctcgaatacc agccagaagc tgctggcgac tacctgcttt
     8461 aatcccatct gcacaagcga taactcgctt tgcccagagg atgcactcgc atcctcttta
     8521 cgcaaaatca gcaatttttc ctggttctca ctgttcactt ttgctgcaaa attattccgt
     8581 ttcgtttgtg ggaataacca tggcagaagc attttacatc ctgataggat ttctgattat
     8641 ggcggcgatt atcgtcatgg ctgtccttta cctcgaaaac cactcctgac gatcatattg
     8701 ttccccccgg catcgcattc accagacctg aaaccgcctt ctgcgccgcc tctgcgcttt
     8761 gtgcaccggg taccagaatc accttacggc actcctcttc acgtttttcg aaaatctcat
     8821 atccccgggc agcctcttca aacggcatat agtgggtaac aatttcttct ggtttcagta
     8881 atcctttctc aattaacggt aataattctc ccagccatgc gtgaacgtgg gtctgtccca
     8941 ttttaaacgt caacccttta tcaaaggcgt cgccaaacag gaaaccgtga ataaatccag
     9001 cgtagacgcc cggtacgcta acaatgccgc cacgcctgac cgccgcaata cactggcgca
     9061 acgctttacc gctgctgccc tccagtttca ggttagtcag caccgtttcc gtggtgctgc
     9121 ctttcgcttc aaaaccgacg gcgtctatta ctgcatccac gccccggtga cctgccgttt
     9181 gttcaataat tgactgtgcc ggatcgctat cttcatcgaa attaatcggg atcgcgccgt
     9241 agcggtcagc ggcgaaacgc aagcggtagg gatgatgatc aacaacaaaa atctgttccg
     9301 caccgagcaa ccgtgcacag gcgattgtca acaatcccac aggaccggca ccatagactg
     9361 caacgcttga accttgttgg atctgcgcat tttttgctgc ctgccacgcc gttagcagaa
     9421 tatcagaaag gaaaagggct ttatcatctg aaagcaaagg cggtacttta aacggcccca
     9481 cattcccttt agggacgcgg acatattccg cctgcccacc aggaacgccg ccatacaggt
     9541 gactataacc aaacaatgcc gcgggtgctg gtatctgttt tttattgagc gcagcgcctt
     9601 tacccgcatt ggtattttcg caggcggcat attgctgtaa tcgacagaaa aaacagtcgc
     9661 cacaagcaat gacgaacgga attaccaccc ggtcgccttt ttgcaaattt tttacgtcct
     9721 tcccggtttc aactacttcc cccataaatt catgaccaaa aatatcgcca tgtttaacct
     9781 ggggtatttt gcctcgataa agatggaggt cagagccaca gatcgccgtt gccgtaatac
     9841 gcagaataat atcatctgcc tgttcaatgc ccggatcggg aacattttct acctgaacgt
     9901 gatgtgggcc gtgataagtc aatgctttca tgcaacctcc agagacagtt caaaaaagag
     9961 tcagtcttta agggtagcta tcacagcggg aaaacgggcg gtaccgcagg ttttgggaga
    10021 attcctgatg ttcagtgaga atatattcat cacagaaacc gttcgtaagg aaaaagaacc
    10081 agacaaatat cagggataaa tattataatc atggcgaatg atttgttcat gattaacagg
    10141 gagaaagggt tatgtataag acaatcatta tgccagttga tgtatttgaa atggaattga
    10201 gcgacaaagc tgttcgccac gctgaattcc tcgcccagga tgacggagtt attcatctac
    10261 ttcacgtact acccgggtca gccagcctga gcctgcaccg ttttgccgct gatgtgcgtc
    10321 gttttgaaga gcatctgcaa catgaagcag aagaacgtct gcaaacgatg gtcagccact
    10381 tcaccatcga tccttcccgc attaaacaac atgtccgttt tggtagcgtg cgggatgaag
    10441 tcaatgagtt ggcagaagaa ctgggggctg atgttgtagt tattggttct cgcaacccat
    10501 cgatttcgac ccatctgtta ggttctaacg cctcgagcgt gatccgccac gccaatctgc
    10561 ctgtactggt cgttcgctaa cagataaaag tatttatgcc accctgcctg gaacatcccg
    10621 gcagggcttt ttaatgccat aaaaaagccg ccaggtttga ccccggcggc taagcaattg
    10681 caggtgaatc ttacttcttc ttatgcagtt ttggtgcgaa tcaggtagtc aaaagcactc
    10741 agagaggctt tggcaccttc gccagtggcg atgatgatct gcttgtacgg aaccgtcgta
    10801 cagtcacccg ctgcgaatac gcctttgacg ttggtttcgc atttcgcatc aatgataatc
    10861 tcacccatgc ggttacgttc gactgcgcct tcgagccagt tggtgttcgg cagcagacca
    10921 atctggacga aaataccggc cagttcgatg ttgtgaatat cgccgctgac gcgatcacga
    10981 tattccagac ctacgacttt gctaccgtcg cctttcactt ccgtggtttg tgcattcaga
    11041 ataatgtcga cgtttttcag gctgcgcagt ttgtcctgca gcacctggtc ggctttcatt
    11101 tctggcgcaa attccagcag agttacgtgc tcaacgatac ccgccaggtc gattgccgct
    11161 tccacgccgg agttaccgcc gccgataacc gctacgcgtt tgcctttaaa cagcgggccg
    11221 tcgcagtgcg ggcagtaggt cacgccttta gtgcgatact gatcttcgcc cggaacgttc
    11281 atgttgcgcc attttgcacc ggtcgccacg ataatgctgc gtgctttcag taccgcgcca
    11341 gaagctgttt caatctggtg tagaccacct tcaaccgctg ccgggatcag tttgctggcg
    11401 ctctggctgt cgatcacatc aacgtcgtat tcatcaacgt gaactttcag cgcacctgcc
    11461 agtttctggc cttcggtctt cggtacagaa atgtagtttt cgatatcaac ggtatcgagg
    11521 atctgaccac caaaacgttc gcccatcaga ccggtacgga tgcctttacg tgcggagtaa
    11581 attgctgccg ctgcacccgc cgggccggaa ccgacgatta atacgtcata agcatcacgc
    11641 ttgttcagct cttctgccgc acgtttttcc gcgcccgtat caattttggc aacgatttca
    11701 gtcaacgtca tgcggccctg accaaactct ttcccgttta cgaacactgc cggaacgccc
    11761 atcacgttgc gatcggtgat ttcgttctgg aaggtgccgc cgtcaattgc agtgtgcttg
    11821 atgcgcgggt tcagtacgct catcaggttc agcgcctgca ccacgtccgg gcagttatgg
    11881 caagagagcg agtaataggt ttcgaattca aaatcaccgt caatatggcg aatctgctcc
    11941 agcagagact gcgcttcttt cgacggatga ccaccggtcc acagcaacgc cagtaccagc
    12001 gaggtgaact cgtggcccag cggggagcct gcaaaacgtg gcccctggtt ggaacctggg
    12061 ttggtgatca ggaaagacgg cttacgcacc ggcaagctgt tatcttcttt aaaggtgact
    12121 ttgtctgaca gttctgcgat ttcagccaac agttccttga tttctgccga tttagcgctg
    12181 tcatccagcg tggcaattaa ctcaacaggc ttggtcaatt tctcaaggta agccttgagt
    12241 tgagttttca tatttgtgtc gagcatgttt atctcctggg ctttaaacat catcatgcaa
    12301 gcggcctctg cggctacctg gatgcagctt acatcatggt gcggggaaga aaatcgggtg
    12361 ccgcagcacc cgaagaatta ggtgaatttc cgaagtattt cgcgccggag ttggtagcgc
    12421 gaaagacgaa ggaaatttag attttaccaa ccaggtccag agacggagcc agagttgctt
    12481 caccttcttt ccatttagcc gggcaaactt cacctgggtg agaagctacg tactgtgctg
    12541 ctttgatttt acgcagcagg tcagacgcat cacggccaat gccttcagcg gtaacttcga
    12601 ttgcctggat gataccctgc gggtcaacaa cgaaggtcgc acggtcagcc agaccttcat
    12661 cttcacgcat gttgtcgaag ttacgggtca gggcgccagt cgggtcgccg atcatcgcat
    12721 atttgatttt agcgatggtt tcagagctgc tgtgccatgc tttgtgggtg aagtgagtat
    12781 cggtagatac tgcgtatacg tctacgccca gtttctgcag ttcttcgtag tggtcagcaa
    12841 cgtcacccag ttcggtcggg catacgaaag taaagtcagc cgggtagaag aagaagacgc
    12901 tccagcggcc ttcggtatct ttttcggtga tttcgatgaa ttcgccgttt ttgaatgcct
    12961 ggtttttaaa aggtttaatt ttggtgttaa tcaaggacat ctatacttcc tccgtgtttt
    13021 cgatgagatg taaggtaacc aatttttgcc gattcggcta atgcgtttcc attatcaaat
    13081 cgataagttt taccttacaa ccttcgtaag acaactttgt gaactttgca ccgccagcgg
    13141 cgataaaaag taaaatggcc ccctttccag gtgaccgtgt aatctgaact accctttggt
    13201 atcttcagcg cctgcctgca ggctgaggtg ttgcccttac aaatgcaaca acgacatgga
    13261 ttacaacacc ctcataaaca aagggcaatc acctgatcta agctcttacc tatgacagtg
    13321 ataggttatg ccttttactc gacttttgca ctgactgaaa aggacaaatt aatgttaaaa
    13381 aagatacttt tactggctct gcttcctgca atcgccttcg cagaggaact tccttcccca
    13441 gtaaaagcga ttgaaaaaca gggcattaca atcatcaaaa cgttcgatgc ccccggagga
    13501 atgaaaggtt atctcggaaa gtatcaggat atgggcgtca ccatctacct gactcccgat
    13561 ggtaagcacg ctatctctgg ttacatgtac aacgagaaag gtgaaaacct gagtaacaca
    13621 cttatcgaaa aagaaattta cgcaccagcc ggacgcgaaa tgtggcaacg gatggaacaa
    13681 tcccactggc tcctcgacgg taaaaaagat gcgccggtca ttgtctacgt cttcgccgat
    13741 ccgttctgcc catattgtaa acagttctgg cagcaggcgc gcccgtgggt agattctggc
    13801 aaagtgcaat taagaacatt gttggttggg gttatcaagc cagaaagccc ggcgacagca
    13861 gcggcaattc ttgcctccaa agatcccgca aaaacctggc aacaatatga agcctctggt
    13921 ggcaagctta agctaaacgt gcctgcaaac gtaagtacag agcaaatgaa agtgttaagt
    13981 gacaatgaga aactgatgga cgatctgggg gcaaatgtca cgccggctat ctattacatg
    14041 agtaaggaaa atacgctgca acaggccgtg gggttgcccg atcagaaaac gcttaatatc
    14101 attatgggga ataaataaaa gataactcat ggatggttga aatatatctc tgcttttcaa
    14161 ccatcctaaa aaagcatgaa ttgctcgctc tgccctaagt ggctgtgcta ttttagaaaa
    14221 acttaatcct gagttaagca aaaaaacata atcaataaaa atacatacgc aaaaaacatt
    14281 gattaagtga atataccatg gaagaaaaat ataaaccgga gtagtgtatg gccaatctct
    14341 acgacttgaa aaagttcgac ttaaatcttc ttgtcatatt tgagtgtatt taccagcatt
    14401 taagcattag caaagctgct gaatcactat atatcacccc atcagccgtg agccagtcgt
    14461 tacaacgctt acgtgcgcaa tttaatgacc ctttattcat tcgctcagga aaaggtatcg
    14521 cgccgacgac aacagggttg aatctgcatc accatcttga gaaaaacctc aaaggccttg
    14581 aacaaacaat taatatagtc aataagtccg aactaaagaa aaactttatt atctacggcc
    14641 ctcagctcat ttcttgctct aataatagta tgctgatccg ctgcctacgg caagatgcct
    14701 ctgtcgagat tgagtgccat aatatcctca tgtcagcaga gaatgctgaa gaactgttgg
    14761 ttcaccgcaa agccgatctg gtgatcaccc aaatgccagt gatcagccgt tccgtcattt
    14821 gcatgccatt gcatacaatt cgcaatactc taatatgtag taataagcat ccgcgaatta
    14881 ctgacaatag cacttatgaa caaattatgg cagaagagtt cacccaatta atatccaaat
    14941 cagcaggagt tgatgatatt caaatggaaa tagacgagaa gtttatgaat cgcaaaattt
    15001 catttcgtgg ttcctctttg ctgacaataa ttaatagcat tgcagttaca gatttactgg
    15061 gaatagtccc ctatgaactt tataactccc accgtgactt tcttaattta aaagagataa
    15121 aactagaaca tccgttacct tcaataaagc tttacatttc ctataataaa tcttctttaa
    15181 ataatttggt gttctcccga tttattgacc gtctaaatga gagtttttga tatataacta
    15241 cagacagcta ctataacttt catctattta ttcacagcgc gaatgaaaaa taaaacactc
    15301 tgctttacag tgtttacttt ataattaaca gtcttatctc actgttcacc ctgtaacgtt
    15361 cagagagcgt cttcagatgt ctatttataa aattcctctt ccgctcaata ttctggaagc
    15421 tgcacgagag cgtattacct ggacactcaa caccctaccc tgcgtttgtg tctcattttc
    15481 tggcggcaaa gactctggtt taatgctgca tctgacagcc gaacttgccc gacaaatggg
    15541 caaaaaaatc tgcgttttgt ttatcgactg ggaggcgcaa ttctcttgca ccattaacta
    15601 tgttcagtcc ctgcgcgagt tgtacaccga tgtcatcgaa gagttttact gggttgcgct
    15661 cccgcttacg acgcaaaatt ccctttcaca ataccaaccc gaatggcagt gctgggaacc
    15721 tgatgtcgaa tgggtgcgtc agcccccgca agatgcgata accgatcctg actttttctg
    15781 cttttaccag ccaggcatga ccttcgaaca atttgtacgt gagtttgccg aatggttttc
    15841 acaaaaacgt ccggcggcga tgatgatcgg catccgtgcg gatgagtcct acaaccgttt
    15901 tgtcgccatc gccagtttaa ataaacaacg ttttgccgac gataaaccat ggactacggc
    15961 cgcaccaggc ggtcatagct ggtacattta ccccatttac gactggaaag tggctgatat
    16021 ctggacctgg tatgcaaatc atcaaaatct ctgcaaccca ctgtataacc taatgtatca
    16081 ggcaggcgtt cctctgcgtc atatgcgaat ttgcgaacct tttggcccgg agcaacgaca
    16141 aggattatgg ctctatcacg ttatcgaacc ggatcgctgg gctgctatgt gcgcacgagt
    16201 cagcggggta aaaagtggcg gcatttacgc cggacatgat aatcatttct atgggcatcg
    16261 gaaaatcctc aagccagaac atttagactg gcaagaatat gcattattgc tgctcaatag
    16321 catgccggaa aaaacagctg agcattaccg caataaaatc gccatttatt tgcactggta
    16381 tcagaaaaaa ggcatcgagg ttccacaaac ccagcaaggg gacattggcg cgaaagatat
    16441 cccctcctgg cggcggatat gcaaagtttt actcaataac gattactggt gtcgggcatt
    16501 atcatttagt cctacgaaag cgaagaacta tcagcgttat aacgaacgga taaaaggaaa
    16561 acgtcaggaa tgggggatac tatgcaacaa cgattaacgc aggatttaac gcaatttctc
    16621 gccagcttgc cggaagatga tcgcatcaaa gcgattaatg aaatccgcat ggcgatccat
    16681 caggttagcc cctttcgtga agagccagtg gattgcgttc tgtgggttaa aaacagccaa
    16741 cttatgccta atgattacaa cccaaacaac ggggcaccac ccgaaaaaaa actgctgcaa
    16801 aaatccattg aaatagatgg ttttacccaa cccattgtgg tcacacatac ggataaaaat
    16861 gccatggaaa tcgtggatgg ttttcaccgc catgaaattg gtaaaggctc atcatcatta
    16921 aaattacgct taaaaggtta tttgccggtg acctgtctgg aaggtacgcg taatcaacgc
    16981 atcgccgcga ctattcgtca taatcgcgct cgtggacgcc atcaaatcac cgccatgtcg
    17041 gaaatcgtcc gtgaacttag ccagttggga tgggacgata ataaaattgg caaagagctg
    17101 ggaatggaca gcgacgaagt gctgcgcctg aagcaaatta acggcctgca agagctgttt
    17161 gccgaccgtc aatattcccg cgcctggacg gttaaataac taaagctggc gcaggcgttc
    17221 agctgctgcc agcaacgtcg attccttctt ggcaaaacag agacgaatca gtttatgggg
    17281 gaagggatcg gcgcaaaata ccgacagcgg gatcgccgct acaccgtgct cccgcgtcag
    17341 ccactggcaa aactcaacgt catccagcga agaaaccgcg ctgtaatcca ccagcaaaaa
    17401 gtatgtacct tcgcacggta aaatctccag ccggctttca tttaaagcat tcaccagaat
    17461 atcgcgcttc tggcgataaa agtccggtaa cgcaagataa tgctcaggtt ctgcacgtag
    17521 catatccgca attgccagct gtgccggggt atttaccgaa aaggtcagat actgatgtac
    17581 cttgcgaatt tcggcactga tgggcgctgg cgcaacgcaa taaccgactt tccagccggt
    17641 catatgatag gtcttgccaa acgatgacac cgccaccgcc cgctcacgca actgcggatg
    17701 cgccagcaca ctggcatggc cctgttgtga aaagttgatg tgctcgtaga cttcatcgct
    17761 aatgacaaaa atctcgtgcc cggcgatcgc ctgccacaaa gcggcgaaat cagcctgctg
    17821 ccagacagtt gcactggggt tatgcggagt gttgaggatc accagtctgg tgcgctcgct
    17881 taataatgcg gcaaattcct gccagtcaac gcgaaaatgc ggtggttgca gtgccatacg
    17941 cttcactatt cccccagaaa gcgcgatggc gggggcgtaa ctgtcatagc tgggatcaaa
    18001 acaaatcact tcatcgccat tgcgcaccag tgcggtaatc gccgcgtata acgcttccgt
    18061 cgcccctgcc gttacggtga tatcgctatc ggcatctggt tgatagccat acaaacgttc
    18121 cgttttctga gcaatcgcct cgcgcaaggc atgcacgccg gtcatgggcg cgtattggtt
    18181 tgccccctgg gcaacgtggt acgccagccg ctcctgtaaa tagcgcggac catcaaaatc
    18241 aggaaagcct tgcgacaggt taatcgcctg gtgttgctgc gccagcgcgc tcatctgggt
    18301 gaaaatagtg gtgccaagtt gtggaagttt gctttgtgga atcagagggt tatttgtcat
    18361 ttttattgta cctgacgaag ttgcgtgttg aaggcactat aacacgatgc tagtatttgg
    18421 caatcaagac gtttagatgt ctaaataaaa caataaggac aacacaacat gcctcacaat
    18481 cctatccgcg tggtcgtcgg cccggctaac tacttttcac atccaggaag tttcaatcac
    18541 ctgaacgatt ttttcactga tgaacaactt tctcgcgcgg tgtggatcta cggcaaacgc
    18601 gccattgctg cggcgcaaac caaacttccg ccagcgtttg gacttccagg ggcaaagcat
    18661 attttgtttc gtggtcattg cagcgaaagc gatgtacagc aactggcggc tgagtccggt
    18721 gacgatcgca gcgtggtgat tggcgtcggc ggcggcgcac tgctcgacac cgcgaaagcc
    18781 ctcgcccgcc gtctcggtct gccatttgtt gccgttccga cgatcgccgc tacctgcgcc
    18841 gcctggacac cgctctccgt ctggtataat gatgccggac aggcgctgca ttatgagatt
    18901 ttcgacgacg ccaattttat ggtgctggtg gaaccggaga ttatcctcaa tgcaccgcaa
    18961 caatatctgc tggcggggat cggtgacacg ctggcgaaat ggtatgaagc ggtggtgctg
    19021 gctccgcaac cagaaacgtt gccgctaacc gtgcggctgg ggatcaataa tgcgcaggcc
    19081 attcgcgacg tcttgttaaa cagtagcgaa caggcacttg ccgatcagca aaatcagcag
    19141 ttaacgcaat cattttgcga tgtggtggat gcgattatcg ccggaggtgg aatggtcggt
    19201 ggtctgggcg atcgttttac gcgtgtggcg gcggcccatg ccgtacataa cggtctgact
    19261 gtcctgccac aaaccgagaa atttctccac ggctccaaag tcgcctacgg aattctggtg
    19321 caaagcgctt tgctgggtca ggatgatgtg ctcgcgcaat taactggagc gtatcagcgt
    19381 tttcatctgc cgactacgct ggcggagctg gacgtggata tcaataatca ggcggagatc
    19441 gacaaagtga ttgcccacac cttacgtccg gtggagtcca ttcattacct gccggtcacg
    19501 ctgacaccag atacgttacg tgcagcgttc gaaaaagtgg aatcgtttaa agcctgacat
    19561 gcaaatttag cagcagcgcg cgccgccttt tccaccgtag cgagcgtcct gccgctcacg
    19621 gaaaaactct tcgtaggtca tcggcgtttg atcgggatgg ttaacccgca tatgttcgac
    19681 atagttgtcg taatcaggca taccaatcat cagcttcgcc gcctgaccta aatattttcc
    19741 ggcttttgcc agtgaatcaa acatcttgac ctccgctaac catgttgctg aaagagtgcc
    19801 ggatgcggcg tgaacgcctt atccggccta caaatgctat caacgcgctc ggtcagtccc
    19861 ctcgccccac cggggagagg gtgaggggga accctcggcg ccatgccaac attcgccaac
    19921 attccccttc actctgactt tagtgcgcgc ctttcgcctg cgccacgatc tcctcgacat
    19981 tttccggcat cggctcatac ggcgtttctt tcgccgttgg cttcggatct ttcaatgccg
    20041 caagtgccgt cttaatcgag aacagcgcca gaaccaccac gaccaccata aagaagatgg
    20101 ttaacccggc atccagacgg ttgttgaaca ccagttgcgc cagttgtgac tcagtatact
    20161 gcgacggaat attgccgctg tcgatcattg cctggaactt gttagcaatg gccaggaagc
    20221 cgactttcgc atccgggcta aacgctttct gccagcctgc tgtcagggta caaatcaaca
    20281 gccaggcggt tggcaccagc gccacccagg cgtaacgttg acgtttcatc ttgaacaaca
    20341 ctacggcaca gagcatcagc gccatccctg caagcatctg gttggcaata ccaaacagcg
    20401 gccacaaggt attaataccg cccagcggat cgaccacgcc ctgatgcagg aagtagcccc
    20461 acgccagcac gcacagcgct gttgccagca ggttagcagg cagtgaatcg gtccgtttca
    20521 ggcccggaga caccacgccc agcagatcct gcaacataaa gcgcgcagca cgcgtacctg
    20581 catccaccgc cgtcagaata aacagtgctt caaacagaat ggcgaagtga taccagaacg
    20641 ccacatccat catgccgccc agcgcgccgt gcagaatgta ggccatcccc accgccaacg
    20701 tcggcgcacc gcccgcacgg gaaatgatcg actgttcacc cacttcgctg gcaatctggt
    20761 tgagcgtatc tggtgtaatg ctaaagcccc agctactcac cacctgcgcg gcagaagcga
    20821 ctacatccgc cgtccctgcc ggagccagca ccgccattgg gctgttcatg gcaaaataca
    20881 cgcccggatc gatgatacag gcagaaacca gcgccataat cgccacgaag gattccatca
    20941 acatcccacc gtagccgata aagcacgcct gcccttcgtt cgccagcatc ttcggcgtgg
    21001 tgccggaaga gatcagcgca tggaagccag acaccgcacc acaggcgatg gtgataaaca
    21061 ggaacgggaa caggttaccg gtccataccg ggccagtgcc atcgacaaat ttggtcagcg
    21121 caggcatggt cagcgtcggg cgcataatca aaatgcctac cgccagaccg acgatagtcc
    21181 cgattttcag gaaggtcgag agatagtcac gcggggccag cagcaaccac accggcagca
    21241 ccgccgccac aaaaccgtag cccaccagca tccaggtcag ctgcacgccg gtaaagtcaa
    21301 agtacggtgc ccaggtcgga ctttctgcca cccagccgcc agagataatg gcgaaaatca
    21361 ggaacaccag gccaatcact gacacttcgc caatgcggcc cggacgcaga tagcgcaggt
    21421 agattcccat aaaaatcgcc agcggaatgg tgaacgcaac ggtatacgtt ccccacgggc
    21481 tatgagtcag ggctttcacc acgatcatcg ccagtactgc aaggataatg accatgatca
    21541 taaagcaggc taccagcgca atcaccccgg cggttggccc catctcttct ttgaccagct
    21601 cacccagcga gcgaccgtca cggcgcgtag aaacaaacag caccatgaaa tcctgcaccg
    21661 caccggcgag aaccaccccg gcgagcagcc agatcatccc cggcaggtag cccatttgcg
    21721 ctgccagcac cggccccacc agcggccctg ctccggcaat ggccgcaaaa tggtgaccga
    21781 acagcacttt cttgtccgtc agcacatagt ccagcccgtc gttatggcgc accgctggcg
    21841 tcatacgcgt cgggtcaacc gccagcacat ttttggcgat atacagccca taaaaacggt
    21901 aagcgatcag atagatacag accgacgcca ccacaatcca cagcgcgttg atctgttccc
    21961 cacgatttaa agcaatgtat cccagagcaa atgctcccat tacagagagc actgtccaga
    22021 cgaggtattt ccctgatttg ttcatagttg ttatccgtgt gcgtgtccat agagatgtta
    22081 cattttgttt ctataacatc tttgtacgat ttaacaacac ggtaaccatg taaatcggag
    22141 ttgaaaccaa tatttaactt aacaatgtta actcgatcac tccgttaacc gagttttcct
    22201 gcaatctcat ttaattctgt cgggctcatc ccaaaactgc cgtacccagc cgacaagtgc
    22261 agcaacgccg cccctgttca tcgaaaacga cgatttccca gctctgattt tgccgcccaa
    22321 gatgcagcgg ctggcagacg ccgcgtacct tgccttcaga caccgggcga tgatgggtgg
    22381 cgttaagttc cgtgcccacc acacactgcc cgtcacgggt catcataaat ccggccatcg
    22441 atcccagcgt ttcagccagc gccgccgacg cgccgccatg cagcaggcca aacggctgat
    22501 gagtacgggt atcaaccggc atttcggctt ccagcacatc atctcccaga cgggtataca
    22561 caattcccag atgcgccacc attgtgttat cgctggtggc gttcagttcg tcgagcgtta
    22621 aatggcgttt ccagatcatg cttatgcccc cagcgttgag ccgccatcga ccacaatatc
    22681 ctgtagggtg atatggctgg cgaggtcaga ggcgaggaac aaaatcgtgt tggcgatctc
    22741 ttgtggacgg gcgattttcc ccagcggaat gccgagttta aactgctcgc caaagccgcg
    22801 aatacgctgt tcttcggcgt catcgctcac ccacagcgtg cgttgcatat cggtgtcggt
    22861 ggagccaggc gaaaccacat tacagcgcac gccgctaacc gccagttcca gcccgacgct
    22921 caacgccagg cttttcagcg ccgctttcga tgcgccataa gcactcatac caatacgcgg
    22981 cgtgtgtgcg gcgtcggacg ccacagtgac aatcgccccg ccccgctgac ggcgaaactg
    23041 gttcatggtt tgctgaaaca ggttaaacgc accgccgacg ttaaccgcaa aagtctgctg
    23101 ccagtcctct ttactgagct gatcggtcgc gcccatgcgc aaaattcccg ccgcattgac
    23161 cagcacgtcc agtcgctccg tttcagctaa cagtcgctga catacctgcg cgacctgcgc
    23221 agcatcggca acatccatca cttcggtcgc aaagggatat tgctcctgag cgaacgcttg
    23281 atcaaaaccg gtaactttcg ctcccgcttc aacaaacgcc agcgccgtgg cgtagccgat
    23341 acctttacct gcgccggtta cccagacatt tttaccgctg aaatccatta tttcacctcg
    23401 cgggagagta gcttccacca ggcgtcgatg gtcgggtttt tcgccagcat gacaaagtcg
    23461 atatcgccat gcactttgcg ccagcgcgcc gccagcgcca tcatgcgcac cgaatccaga
    23521 ccgtagtcga tcaggttgtc gtcatcgaac ggttcatcgg actcgtccag caacggcagg
    23581 ataacctcac gcagcgccgc tttgctggcg gggacaggtg ctggcagtaa ttcttcagtc
    23641 atcaccaccc ggccagaccg cccggccaca tatttcagcg acatcaaatg ctcgtcacgg
    23701 ttgaaatcgg ccagcgcatc cgccaccata aacggtttaa tatcgcgcat aaatgcgtcg
    23761 gttgcggtgg tcatacagcc aatgtgggca tataccccgg taataatcag ctggttacgt
    23821 ccactctctt tgagcatttg ctccagcgga gaacgatgaa acgcgctgta gcgccacttc
    23881 accagcaccg tgtcgtcggc atctggcgtc aggcgatcca ccaccttttg ctgttccggc
    23941 gaacgggtca ggcccggccc ccacatatca ttcaacagcg cccgatcttc atcgctctgc
    24001 tctttcggct gggcggtgta ataaaccggg atattgtgct gtttgcagta gtcgcgcagc
    24061 gcagcaatat tcgcgatcac ctgctccatc atcgggcagt tctcgcccca gaagctgaca
    24121 aaatagtcct gcatatcatg gattaacaac gcggcacgtt gcggttcaaa ggcccagtca
    24181 actttattct gcggaatatc gtgagactcc ggcagtgcgt aagcctgtaa ttttggaata
    24241 gccatcgtgt tctctccttc aggctgatgc gcgtgacgcc agccactgac gtaattgttt
    24301 tttatcgact ttcccgaccg ccgtcagcgg aagtgaatcc acacactcca cgcgatccgg
    24361 taatttaaat tcggcaatac cctgttcacg caggaaacga cgcacctgca ccgcgcgcag
    24421 cggctctttt accaccagat aagcacagct tttttcgccc atcagctcat cttccatgct
    24481 caccagtgcg gcatagatca ccgccggatg gcgcagcagc aggttttcga tctcttcggc
    24541 agcgatcttc tcgccgccac ggttgatctg atctttctcg cgcccctgca cggtgatgta
    24601 accctctgga tcaatagaga tcagatcgcc ggaacagtaa aaaccgttgg catcaaaggc
    24661 actggcattg tgctgcggac ttttgtaata accgcggaag gtgtacggcc cgcgcgtcat
    24721 caggcgtcca acttcccctt gcggcagtgg atttccttca gcatcagcaa cccatacttc
    24781 atcgtccgga cacattgggt aaccctgggt atggataatt ttctccgcgc tatcatcaag
    24841 tcgggtgtag ttcaccagcc cttccgccat gccaaacacc tgctgcaaca ggcagccaat
    24901 ctcagcggga atacgcgccg caagggtggc tgaaagacgt gcgccgccga cctgtaacag
    24961 tttcagcgag gcaagctgcg cccggctttc accttcggtc agcgcctgca accacaggct
    25021 gactgccggt ggcaccagcg cggtgacgtt cacctgatgt ttttcaatca atgggaagca
    25081 aagcgtggcg ctgggatcgg ccgccagaac aaccgttcct ccggcaagaa agacgcccag
    25141 cgctcccggc gaactcatgg cgtagttatg agccgccggg atcgcgcaca ggtagcgtgt
    25201 ctgttgtgtg aactgacaaa tctcgacgct acgacgcacg ctgtagtagt agtcgttatg
    25261 agtgcgcggg atcagtttcg gtgtgccggt ggtgccgccg gaaagctgga aataggccac
    25321 ttcatcagca ggtgatggcg tggcagtaaa atcctcagcc ggatggttaa tcgcatcctg
    25381 caagttatgc tcaccgctgt cgttgtgcag ttgcaccacg cgaatggagg aatgttctgt
    25441 gacgaaagta tagaggaaat catccccgct aaacagcgca tgttggcgat cggcaatcag
    25501 caatgcgggt tcaatctggc tggcataggc gttcagttca ctacgctgat ggctgaacag
    25561 cgccagcacc ggcgcaacgc ccagtttcag cagcgcgaaa aaggtaatat acaattcagc
    25621 gacgttaccc agttgtacca gcgcggtttc accaggttta atgccctgac ggcgtaaact
    25681 acacgcgagg ttatccgccg cctgattcag ctcccggtaa ctcaactgtc gctcgccgtc
    25741 gataaccgcg atgctgtcac tcgcagcgtg gcgagtcaga atgtcggtca gcggcaaatc
    25801 ctgccagtag cctttttccc gatagcgacg ggcaaactct tccggccagc gggtgaatgg
    25861 aatgctcatc ctcgctcctt aatgcaatcc aaaaacgttc aacatggtag aaagtttgac
    25921 gcctgtttcg cgccactcac ccaacggtga cgacgcaggc acaatccccg ctccggcaaa
    25981 cagacgcacc tgattttccc gcagcttcgc gcagcggatg gtcaccaccc attcgccgtt
    26041 accttcgctg tcacaccaac ccacaatgcc gccaaacagt tcgcggtcga atggctccag
    26101 ttcagcaata acctgggtcg cggcctgatg cgggaaaccg ctcagcgcgg gggttggatg
    26161 cagcagacag gccagagtca gtgcgttttc ttgcgaattc gctttacctt caaagggagt
    26221 tgcgagatgc cacagcgtcg gcgtggtaat caattgtgga gaggagggaa cgtgtaactc
    26281 actactgcgt tcgcgcagta cctctttcat cgcctgagtc accagttcat gttcatggcg
    26341 atctttttct gacgccagca gacgattacc cgcttcgcga tcgagcactt catccggctg
    26401 acgacgcgcg gaaccggcta acggaatgga gctaaaacgc tcgccgtctt tacgtagcag
    26461 cagttccggg ctggccccca gcaggacgcc accatcagcc agcgggacat ggaagttgta
    26521 actaaccggg ttttgcgcaa tcaaccgttc cagcaatacg ccactatcaa tggcggcgtc
    26581 agtggtgata tcaatcaatc gtgacaacac cactttgtcg acctgcggcg tggcggtaag
    26641 tgcggcggcg cgggcaacca tctgttcaaa cgtggtttgt tcaggaattg cctggcgttc
    26701 caccacgttc agcgactggc tgcgggtaaa acggcgggct gaggtctgtt tttcctggcg
    26761 ggagaacgac tgccaggatt cgggaatata cagcgacgaa ggctgacgtg gatcgaaggg
    26821 aatcgccccg accatcaccg gatttttgat gccctgcgct ttggcatcgg caaacagcgc
    26881 ggcgagtttt tgctggaagg gactgtcggg cgaatccccg ttcacagccg gttcatcgaa
    26941 gcgggcgaaa catcctgacg tcgtaaaact gcggtacggc gacataaaga aaaagcgatt
    27001 gggcgcaagt gttgccatgg tctgctgtac ttcctcagcc agtgacgtat ccatatcatc
    27061 ctccacaaaa tgataaaggc tttaataatg attatcattt atattttcgg tcgctaacct
    27121 aaaagcaaac gcgcactatg tcaactcttg aggtaacgca atctgcaact cggggttgca
    27181 tctggttgtg gttacaatga aaatgagaag cattattgat ggattcgcat aagcgcaatg
    27241 tgatgtcctg cgccgttctg ccccctctcc cttccagggt tagggctggg gtgagggtta
    27301 atgttcgcac cagtgctggc tgttcccctc accctaaccc tctccccaaa ggggcgaggg
    27361 gacggattgt gcgctttgtc gaatttgtca ttacgccctt aaccttatta ataacaggaa
    27421 gctgacttgt gagactcgcc ccgctctacc gcaacgccct tctattaaca ggacttttgc
    27481 tttcaggaat agccgtagtt caggccgccg actggccgcg tcagattact gacagccggg
    27541 gcactcatac cctggaaagc cagccgcagc gtattgtttc caccagcgtc accctgaccg
    27601 gatcactgct ggcgattgat gctccggtga tcgccagcgg cgcgaccacg ccgaataacc
    27661 gcgtcgcaga tgaccagggc tttttacgcc agtggagcaa ggtggcgaaa gaacgcaaac
    27721 tgcaacggct ctatatcggc gaaccgagcg ccgaagccgt tgccgcgcaa atgccggatc
    27781 tgattttaat tagcgcaacc ggcggggatt cggcgctggc gctgtatgat cagctttcca
    27841 ccatcgcccc gacattaatc atcaattacg acgacaaaag ctggcagtcg ctgttaacgc
    27901 agcttggcga aattaccggg catgagaaac aagcggcaga gcggattgca cagtttgata
    27961 agcaactggc agcggcgaaa gagcaaatca aattaccgcc gcagccggtc actgccattg
    28021 tctataccgc cgctgcacac agtgccaatc tctggacgcc ggaatcagca caagggcaga
    28081 tgctggaaca actcggcttt acgctggcga agttgcccgc agggttaaac gccagccaaa
    28141 gccagggcaa acgccatgac atcattcagc ttggtgggga aaatctggct gcggggttaa
    28201 acggtgaatc gctattcctg ttcgctggtg atcagaaaga tgccgatgct atttatgcta
    28261 atccgctgct cgcgcacctg cctgcagtac aaaacaagca ggtttatgcg ctgggaaccg
    28321 agacgttccg cctggattac tacagcgcca tgcaagtgct ggacagactt aattcactgt
    28381 ttaaaaaaga ttgataaaac atacaattag agcactaaca atcccctcgc ccctttgggg
    28441 agagggttag ggtgagggga aaatgcacct ttagctaact gtcagacgct gtcacctgcg
    28501 gcggcgtctg gcgaaaacgt cgcaactcca ccagcaccag caacaacaac acgccgataa
    28561 tcaacaaacc aaaaccgctc gcgcttgcag aagcaaccgg tgtcatcatc gctcccagac
    28621 cgcctaacag cgccgcacct atggcatcgc ccgtcacgtt ttgcgccgtc cacaaaccgt
    28681 taatccgccc taacatcgct tccggggttt gcgtttgcag cattgtgtat tgcagcaacg
    28741 agctgaccgc actcaaccag ccgaacagcg ccagacaaac cacgcctaaa atccacatcg
    28801 gcatcaggcc aaacagacca atggcgagga acgatcccag cgtggagagc agcatcaata
    28861 accctggtcg cgcgctatgt gccagcttcc cactggttaa cgcgccaata gccgcaccga
    28921 gcgggatcgc cgcatagaga aaaccaatct gtgccgccga catctgccag ttgtcagcca
    28981 gcgccggata cagtacccgc accgcgctcg ccatcgtcaa taaaccaccc agcaacgcaa
    29041 tccctcctac cagcgggctg gcgagcagaa aacgaaatcc tgccagtaat gatttcaacg
    29101 gatgctcgcg cggctgcggt ggcggtggca acgccggaag gcttaacaac ggtagcaagg
    29161 taataaacgt gcccgccgcc gccagcccgt agttccaggc tacgccaccg gtcgccagca
    29221 ataaaccgcc aatcatgggc gaaatcaccg accccagacg cacggtcaac atggtgatcg
    29281 ccccggcctg cattaagttt tcacgcccta ccagtgcagg tgttgccgcc agtagcgccg
    29341 taacaccaag tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg
    29401 acggctccgg cagcagcgcg ttcaggcaca gtccaatgaa gccaatgcca caggtgccgc
    29461 gcgccagcaa aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca
    29521 ggccaacaaa catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat
    29581 gcgtcatcat ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg
    29641 agataaaacg agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt
    29701 taagcagcca agattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta
    29761 ttcttaggct gcacatgcta acatatccaa ataagatcga taacgataat taatttcatt
    29821 atcatggaag ttcgtatgtc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga
    29881 ttgctgttat tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc
    29941 cccgcttccg tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc
    30001 gtgctcgacg cgcgactgcc gcgcaccctt gccggtttac tggcaggcgg cgcgcttggc
    30061 cttgccgggg cgttgatgca aaccctcacc cgaaatccac ttgccgaccc cggcttgctt
    30121 ggcgtgaacg ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca
    30181 tccgcgcagg aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt
    30241 gcctttaccg gcagtcaggg cggcgggcag ttaagtccgg tgcgtttaac cctggcgggc
    30301 gtggcgctgg cggcggtgct ggaagggctg accagcggca tcgccctgct taatcctgac
    30361 gtctacgatc aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc
    30421 ttaaaagtgg tgctgatccc ggtgctgatc gccggagcaa ccgcgctatt actgagccgc
    30481 gcgctgaaca gtttaagcct cggcagcgac accgcggcgg cgctgggcag tcgcgtggcg
    30541 cgcacacagt tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta
    30601 gttggcccga ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt
    30661 gccgatcatc gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt
    30721 gccgatatca tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg
    30781 tttattggtg caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga
    30841 tttacgtctc tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg
    30901 caggtatctg gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg
    30961 cgttgatgag cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac
    31021 gcgtgctgat ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt
    31081 cgctgatgcg taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga
    31141 gcggcgtgct ggtggcgatg gtgctgtttg gtcaggacct gacggctatc gcgctggcag
    31201 caatggtggg cggcattgtc acttcgctgt tggtctggct gctcgcctgg cgtaacggta
    31261 tcgacacctt tcggttgatt attatcggca tcggcgttcg cgccatgctg gtggccttta
    31321 atacctggct gttgctgaaa gcgtctttag aaacggcgct aacagcgggt ttgtggaatg
    31381 ccggatcgct caatggcctg acgtgggcaa aaacttcgcc ttccgcaccc atcattatat
    31441 tgatgctcat tgccgccgcc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg
    31501 ataccgcctg cgcgctgggc gtcagcgtcg aacgttcgcg tctgttaatg atgctggttg
    31561 cagtggtgct taccgctgcg gcaacagcgc ttgccgggcc gatttccttt attgctttag
    31621 tcgcgccgca catttcccga cgcattagcg gcaccgctcg ctgggggcta acccaggcgg
    31681 cgctatgcgg ggcgctgtta ctgctggcgg ccgatctctg cgcccaacaa ctgtttatgc
    31741 cgtatcaact tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct
    31801 tgttaattca ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca
    31861 gttaaccctg ggatatggca aatataccgt tgcggagaat ctgacagtag aaatacctga
    31921 tggtcacttc acggcaatta tcgggccaaa tggctgcggt aaatccactt tactgcgtac
    31981 cttaagccgc ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacatcca
    32041 acattacgcc agtaaagagg ttgcacgccg gattggtctg ttggcgcaaa atgctaccac
    32101 gccgggcgat atcaccgtgc aggagctggt ggcgcgtggg cgttacccgc atcaaccgct
    32161 gtttacccgc tggcgcaaag aagatgaaga agcggtaacg aaagcgatgc aggccacggg
    32221 aataactcat ctggcggatc aaagcgtgga taccctttct ggcggacaac gccagcgcgc
    32281 gtggatcgcg atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac
    32341 ctggctggat atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga
    32401 gaaaggctat accttggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag
    32461 ccatttgatt gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt
    32521 cactgctgaa ctgattgagc gtatttatgg tctgcgatgc atgatcattg acgatccggt
    32581 agccggaacg ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta
    32641 aactaagtgg tctgccatca tggcatcctg ttttctggct gccatcgcat agcgcaataa
    32701 cacgctacca caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt
    32761 acccggaccg tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa
    32821 attcacatcg ttgatatttg cttttgttaa ctgctcaaca agatactgac gattacgtaa
    32881 ttcaccgttc agttccgcaa cgtcagtgac cgctttttca atttccagtt tgcgttcaat
    32941 accgtctgcg ccgagagaaa tagagaaatc ggggtcatct ttaacggcct gaccattact
    33001 gtatacgggc tttttaattc ccgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg
    33061 aatatttgca tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc
    33121 aaactgggtt ttgatttcca gtttattacg gacgttttct agcgactctt tcaccaccaa
    33181 cgcagagata taatcgatat acccgctcaa aacggtctgc gcctcttcac tggttggcgc
    33241 ggtaaaactt agcgtccagg aggtatacag tgacggttca tcttttttct tactggcatt
    33301 gtcatcaacc gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc
    33361 atcgattttc gcctctttta attggtccat cacataaggt gatgaacgca ggtactcttc
    33421 cagcaagcta accgactgaa acttcttgat aaacaggtta aatgcttctg tacgatcaat
    33481 tttgatatcc agatccagca ctcgaagctt agtgaatgtt ttctccagct cttgccactg
    33541 aacaggttct ggaggcgtga caaccgccgc gctggtccat ttttgcggca ggatgaaaga
    33601 gatcagcaag cctgcgcagg caaacgcaaa aatgaccgcc atgaccgttt ttttggcccg
    33661 ccataaaact gcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg
    33721 atactcagga aaatgagcgt cacttccctg tttaatattc agtgatgaca tggggtataa
    33781 cctgaaaatg agtccaatag gggaaatctc cacggcgctc actttaccag ttggtgataa
    33841 ttatccgaag ctgaagtttg taaattcctt ccactgaatg caatattcac tctatttaag
    33901 agaaatattc atcaaaccta taaatcaaac aaataaaaaa cccataatt
//
LOCUS       DAZKHC020000047        33091 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZKHC020000047 DAZKHC020000000
VERSION     DAZKHC020000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 33091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.047"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /locus_tag="ACHOSM_RS12680"
                     /old_locus_tag="pgaptmp_002536"
     CDS             <1..198
                     /locus_tag="ACHOSM_RS12680"
                     /old_locus_tag="pgaptmp_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU4559033.1"
                     /translation="ENGVFMRNSRGAEICSLYDKDALVQLVETGGAHPLSREPITESM
                     IMRKDECHFDSKKEAFVASDA"
     gene            complement(326..1384)
                     /gene="espW"
                     /locus_tag="ACHOSM_RS12685"
                     /old_locus_tag="pgaptmp_002537"
     CDS             complement(326..1384)
                     /gene="espW"
                     /locus_tag="ACHOSM_RS12685"
                     /old_locus_tag="pgaptmp_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HGU4559034.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMTNSVSRRIVHKEYGISL
                     KSVPVWLATAKTPLALLNGRHTRSHSFIIAGTPGMGSRSGAQYYAINSDDKRSRIDID
                     SLFLKKLNNARNQNKFPIDVKETVIKLQGQKFTCIEDFHKKYNETRLKANTNIQQKQI
                     ADEVKSLTYLIPSEKKEMWIYKNNGKDNAKPNLGERDVRMFENISPDDTDKITGRKFS
                     ELGEYLYSGNVIKLSQLSIRYLPNINSISLIETKQSLLLHRLYSDEVLQRNGTLIPTP
                     LHEEKSIPADNIKTMLNNIPTYKMLPPFTETQGNCSSGAATFLRKSGAEEKDILACSP
                     RNYGLHHNIKTWDPLVRN"
     gene            complement(1459..2109)
                     /locus_tag="ACHOSM_RS12690"
                     /old_locus_tag="pgaptmp_002538"
     CDS             complement(1459..2109)
                     /locus_tag="ACHOSM_RS12690"
                     /old_locus_tag="pgaptmp_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU4559035.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPIPGGLWDHLGARESHHLADSLARQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
     gene            2292..2882
                     /locus_tag="ACHOSM_RS12695"
                     /old_locus_tag="pgaptmp_002539"
     CDS             2292..2882
                     /locus_tag="ACHOSM_RS12695"
                     /old_locus_tag="pgaptmp_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bfpT-regulated chaperone"
                     /protein_id="HGU4559036.1"
                     /translation="MPMNTTGTSFSSFGISCHRENSFRNSFRGKNDEVIKCSMGERTI
                     RFSVRKFSGNILDTVSRQSTKDINGWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFSLVSQEYQLTLDVKAAQSSINHVIMGNASFGKKIDALCDSMSRDVKNRTADSI
                     ANLLADKFYQKHIEPDIDIVKLRNEIPDYLRCAIQA"
     gene            complement(3156..4019)
                     /gene="potB"
                     /locus_tag="ACHOSM_RS12700"
                     /old_locus_tag="pgaptmp_002540"
     CDS             complement(3156..4019)
                     /gene="potB"
                     /locus_tag="ACHOSM_RS12700"
                     /old_locus_tag="pgaptmp_002540"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HGU4559037.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDD"
     gene            complement(4003..5139)
                     /gene="potA"
                     /locus_tag="ACHOSM_RS12705"
                     /old_locus_tag="pgaptmp_002541"
     CDS             complement(4003..5139)
                     /gene="potA"
                     /locus_tag="ACHOSM_RS12705"
                     /old_locus_tag="pgaptmp_002541"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HGU4559038.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            5389..6615
                     /gene="pepT"
                     /locus_tag="ACHOSM_RS12710"
                     /old_locus_tag="pgaptmp_002542"
     CDS             5389..6615
                     /gene="pepT"
                     /locus_tag="ACHOSM_RS12710"
                     /old_locus_tag="pgaptmp_002542"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGU4559039.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(6664..7785)
                     /gene="roxA"
                     /locus_tag="ACHOSM_RS12715"
                     /old_locus_tag="pgaptmp_002543"
                     /gene_synonym="ycfD"
     CDS             complement(6664..7785)
                     /gene="roxA"
                     /locus_tag="ACHOSM_RS12715"
                     /old_locus_tag="pgaptmp_002543"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HGU4559040.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPVLD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            8034..9263
                     /locus_tag="ACHOSM_RS12720"
                     /old_locus_tag="pgaptmp_002544"
     CDS             8034..9263
                     /locus_tag="ACHOSM_RS12720"
                     /old_locus_tag="pgaptmp_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4559041.1"
                     /translation="MSRSLNKLSDTLLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYDPLKKRLNNHVIQHIGPMPLDKCELRHWLACF
                     DRVAKKAPVSAGFLLQTCKQALKFCRKRRYAISNVLDDMSVADVGKKPDISERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGCRTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTEWLGI
                     LAGTHENVTTLPVARRK"
     gene            9628..9816
                     /locus_tag="ACHOSM_RS12725"
                     /old_locus_tag="pgaptmp_002545"
     CDS             9628..9816
                     /locus_tag="ACHOSM_RS12725"
                     /old_locus_tag="pgaptmp_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4559042.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            9874..10446
                     /locus_tag="ACHOSM_RS12730"
                     /old_locus_tag="pgaptmp_002546"
                     /pseudo
     CDS             9874..10446
                     /locus_tag="ACHOSM_RS12730"
                     /old_locus_tag="pgaptmp_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="icd-like protein"
     gene            complement(10533..10637)
                     /locus_tag="ACHOSM_RS12735"
                     /old_locus_tag="pgaptmp_002547"
                     /pseudo
     CDS             complement(10533..10637)
                     /locus_tag="ACHOSM_RS12735"
                     /old_locus_tag="pgaptmp_002547"
                     /inference="COORDINATES: protein motif:HMM:NF041551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10828..11031
                     /locus_tag="ACHOSM_RS12740"
                     /old_locus_tag="pgaptmp_002548"
     CDS             10828..11031
                     /locus_tag="ACHOSM_RS12740"
                     /old_locus_tag="pgaptmp_002548"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4559043.1"
                     /translation="MRNHTTHPQGRDPHNLNKYIWRFIALSTAQPRVITIEASSEQEA
                     RQQSPAGCVMVFAARIRQGVFHA"
     gene            11024..11344
                     /locus_tag="ACHOSM_RS12745"
                     /old_locus_tag="pgaptmp_002549"
     CDS             11024..11344
                     /locus_tag="ACHOSM_RS12745"
                     /old_locus_tag="pgaptmp_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559044.1"
                     /translation="MPDMTNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     NLMWYASENEEYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENAADALNAIKKANTD
                     SNRG"
     gene            11351..11650
                     /locus_tag="ACHOSM_RS12750"
                     /old_locus_tag="pgaptmp_002550"
     CDS             11351..11650
                     /locus_tag="ACHOSM_RS12750"
                     /old_locus_tag="pgaptmp_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4559045.1"
                     /translation="MRTYLSGLTASGYAHPKIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFHSSLNVEIFISRFRREIPTSRNNHVSRVDVDKKLQELKNMIASWREQK"
     gene            11647..13464
                     /locus_tag="ACHOSM_RS12755"
                     /old_locus_tag="pgaptmp_002551"
     CDS             11647..13464
                     /locus_tag="ACHOSM_RS12755"
                     /old_locus_tag="pgaptmp_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HGU4559046.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVWLGEQQLAELDNLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERGELAEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVIWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDP
                     MLAEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCGYLM
                     ASVMCDGLLVGNAEIVPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGKDMPGAMAEYG
                     REYMKRKTKHGLRSNVTLTEESEDWMPSCVSVTNDDSKN"
     gene            13752..13997
                     /locus_tag="ACHOSM_RS12760"
                     /old_locus_tag="pgaptmp_002552"
     CDS             13752..13997
                     /locus_tag="ACHOSM_RS12760"
                     /old_locus_tag="pgaptmp_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559047.1"
                     /translation="MTAKHTKKSQSHALDLTEHWLRVSIKIIDRNAGEGYAKAHPELI
                     SAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            13994..14404
                     /locus_tag="ACHOSM_RS12765"
                     /old_locus_tag="pgaptmp_002553"
     CDS             13994..14404
                     /locus_tag="ACHOSM_RS12765"
                     /old_locus_tag="pgaptmp_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4559048.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVPPPCSQADDGT
                     ATMWLSVLAFGRQAEALARHRKGELLSVAGNMQISQWTGQNGETLQGYQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDGIPF"
     gene            14415..14687
                     /locus_tag="ACHOSM_RS12770"
                     /old_locus_tag="pgaptmp_002554"
     CDS             14415..14687
                     /locus_tag="ACHOSM_RS12770"
                     /old_locus_tag="pgaptmp_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4559049.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECCRKAQRPPEPPDNYGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            14644..14772
                     /locus_tag="ACHOSM_RS12775"
                     /old_locus_tag="pgaptmp_002555"
     CDS             14644..14772
                     /locus_tag="ACHOSM_RS12775"
                     /old_locus_tag="pgaptmp_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGU4559050.1"
                     /translation="MVLVMKYGAITRTANHTAGEIRLFLHQLKRYGALPGFRHGLA"
     gene            14813..15037
                     /locus_tag="ACHOSM_RS12780"
                     /old_locus_tag="pgaptmp_002556"
     CDS             14813..15037
                     /locus_tag="ACHOSM_RS12780"
                     /old_locus_tag="pgaptmp_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559051.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDAMIEFLKGIRD"
     gene            15325..16431
                     /locus_tag="ACHOSM_RS12785"
                     /old_locus_tag="pgaptmp_002557"
     CDS             15325..16431
                     /locus_tag="ACHOSM_RS12785"
                     /old_locus_tag="pgaptmp_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU4559052.1"
                     /translation="MRDMLENAEKENRSLNDAEGAKFDELRAKAESLDKDISRLEAIA
                     DEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGYTVIPELNTEIMRMLADE
                     STMRRICTVKKISSNEFKQLVSAGGAVVNHGEEGKAREQTSTPQINEVSIKLYPVYAY
                     PRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGDKKAKGFLSVPRAEKNDK
                     ERDFGTLQVIKPVDDLAWTSADPLIDLKFALRKKYRKNAVWVVNSTTAAKLQKVKNAN
                     GDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRGYYIVDHETGVRTRPDNL
                     TEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            16487..17044
                     /locus_tag="ACHOSM_RS12790"
                     /old_locus_tag="pgaptmp_002558"
     CDS             16487..17044
                     /locus_tag="ACHOSM_RS12790"
                     /old_locus_tag="pgaptmp_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGU4559053.1"
                     /translation="MKSMEIRSSEITTSASNTLTGYVVRWDNLSELLWGEFYEKFQRG
                     AFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDDTGLRFELTPPDTSTGRDVIE
                     LVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGVELARR
                     SLYEQHPEKMPRADNRRWWADLAGV"
     gene            17046..18257
                     /locus_tag="ACHOSM_RS12795"
                     /old_locus_tag="pgaptmp_002559"
     CDS             17046..18257
                     /locus_tag="ACHOSM_RS12795"
                     /old_locus_tag="pgaptmp_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4559054.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGAKNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEYI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVVSPVPGI
                     RYQVEFDSEDLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            18254..18592
                     /locus_tag="ACHOSM_RS12800"
                     /old_locus_tag="pgaptmp_002560"
     CDS             18254..18592
                     /locus_tag="ACHOSM_RS12800"
                     /old_locus_tag="pgaptmp_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU4559055.1"
                     /translation="MNIGRLRDRVTIQTLKQTRAMTGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVNTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            18589..18885
                     /locus_tag="ACHOSM_RS12805"
                     /old_locus_tag="pgaptmp_002561"
     CDS             18589..18885
                     /locus_tag="ACHOSM_RS12805"
                     /old_locus_tag="pgaptmp_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU4559056.1"
                     /translation="MTEELITLEEVKLHCRIDGDEEDQLISGYIAASLEACQIHIGRR
                     FDDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTHVPMTIGALWTAYRDVGVY"
     gene            18885..19325
                     /locus_tag="ACHOSM_RS12810"
                     /old_locus_tag="pgaptmp_002562"
     CDS             18885..19325
                     /locus_tag="ACHOSM_RS12810"
                     /old_locus_tag="pgaptmp_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HGU4559057.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRSARQQQDSRRGSSRER
                     GYTRQWEKYRAMYLSKNPLCAHCLEKGIYTPAVVVDHIIPIDGGNDVLFWPEWNHQPL
                     CQACHNQKTKWLDPSTKNKRAAGGFREEEERAANRNNWMYGADE"
     gene            19309..19491
                     /locus_tag="ACHOSM_RS12815"
                     /old_locus_tag="pgaptmp_002563"
     CDS             19309..19491
                     /locus_tag="ACHOSM_RS12815"
                     /old_locus_tag="pgaptmp_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559058.1"
                     /translation="MALMNEREQNRLIRGLIRQRDAWKAQETGHKDKASGRAERITAT
                     RLNDRDREVMECFRNR"
     gene            19614..19970
                     /locus_tag="ACHOSM_RS12820"
                     /old_locus_tag="pgaptmp_002564"
     CDS             19614..19970
                     /locus_tag="ACHOSM_RS12820"
                     /old_locus_tag="pgaptmp_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HGU4559059.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            19954..21615
                     /locus_tag="ACHOSM_RS12825"
                     /old_locus_tag="pgaptmp_002565"
     CDS             19954..21615
                     /locus_tag="ACHOSM_RS12825"
                     /old_locus_tag="pgaptmp_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4559060.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLESPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLTGKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKNIMLVSRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPGVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            21629..21910
                     /locus_tag="ACHOSM_RS12830"
                     /old_locus_tag="pgaptmp_002566"
     CDS             21629..21910
                     /locus_tag="ACHOSM_RS12830"
                     /old_locus_tag="pgaptmp_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559061.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDVDMRDIRIRVLEMLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            22191..22349
                     /locus_tag="ACHOSM_RS12835"
                     /old_locus_tag="pgaptmp_002567"
     CDS             22191..22349
                     /locus_tag="ACHOSM_RS12835"
                     /old_locus_tag="pgaptmp_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559062.1"
                     /translation="MVDGGVADLQLDHETTETTRSLGSKSNTQILGLTRDTQIWGIGG
                     NHNDFRYG"
     gene            complement(22693..24153)
                     /gene="phoQ"
                     /locus_tag="ACHOSM_RS12840"
                     /old_locus_tag="pgaptmp_002568"
     CDS             complement(22693..24153)
                     /gene="phoQ"
                     /locus_tag="ACHOSM_RS12840"
                     /old_locus_tag="pgaptmp_002568"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HGU4559063.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(24153..24824)
                     /gene="phoP"
                     /locus_tag="ACHOSM_RS12845"
                     /old_locus_tag="pgaptmp_002569"
     CDS             complement(24153..24824)
                     /gene="phoP"
                     /locus_tag="ACHOSM_RS12845"
                     /old_locus_tag="pgaptmp_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HGU4559064.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(24993..26363)
                     /gene="purB"
                     /locus_tag="ACHOSM_RS12850"
                     /old_locus_tag="pgaptmp_002570"
     CDS             complement(24993..26363)
                     /gene="purB"
                     /locus_tag="ACHOSM_RS12850"
                     /old_locus_tag="pgaptmp_002570"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGU4559065.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(26367..27008)
                     /gene="hflD"
                     /locus_tag="ACHOSM_RS12855"
                     /old_locus_tag="pgaptmp_002571"
     CDS             complement(26367..27008)
                     /gene="hflD"
                     /locus_tag="ACHOSM_RS12855"
                     /old_locus_tag="pgaptmp_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HGU4559066.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(27044..28150)
                     /gene="mnmA"
                     /locus_tag="ACHOSM_RS12860"
                     /old_locus_tag="pgaptmp_002572"
                     /gene_synonym="trmU"
     CDS             complement(27044..28150)
                     /gene="mnmA"
                     /locus_tag="ACHOSM_RS12860"
                     /old_locus_tag="pgaptmp_002572"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGU4559067.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(28204..28665)
                     /gene="nudJ"
                     /locus_tag="ACHOSM_RS12865"
                     /old_locus_tag="pgaptmp_002573"
     CDS             complement(28204..28665)
                     /gene="nudJ"
                     /locus_tag="ACHOSM_RS12865"
                     /old_locus_tag="pgaptmp_002573"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HGU4559068.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(28675..29328)
                     /gene="rluE"
                     /locus_tag="ACHOSM_RS12870"
                     /old_locus_tag="pgaptmp_002574"
     CDS             complement(28675..29328)
                     /gene="rluE"
                     /locus_tag="ACHOSM_RS12870"
                     /old_locus_tag="pgaptmp_002574"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HGU4559069.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            29500..30750
                     /gene="icd"
                     /locus_tag="ACHOSM_RS12875"
                     /old_locus_tag="pgaptmp_002575"
     CDS             29500..30750
                     /gene="icd"
                     /locus_tag="ACHOSM_RS12875"
                     /old_locus_tag="pgaptmp_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HGU4559070.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(30864..32006)
                     /locus_tag="ACHOSM_RS12880"
                     /old_locus_tag="pgaptmp_002576"
     CDS             complement(30864..32006)
                     /locus_tag="ACHOSM_RS12880"
                     /old_locus_tag="pgaptmp_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGU4559071.1"
                     /translation="MAARPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYISIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLSEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLGDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(31996..32232)
                     /locus_tag="ACHOSM_RS12885"
                     /old_locus_tag="pgaptmp_002577"
     CDS             complement(31996..32232)
                     /locus_tag="ACHOSM_RS12885"
                     /old_locus_tag="pgaptmp_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4559072.1"
                     /translation="MSRLITLQDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            complement(32372..32611)
                     /locus_tag="ACHOSM_RS12890"
                     /old_locus_tag="pgaptmp_002578"
     CDS             complement(32372..32611)
                     /locus_tag="ACHOSM_RS12890"
                     /old_locus_tag="pgaptmp_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4559073.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRAIRRK"
     gene            complement(32659..32877)
                     /locus_tag="ACHOSM_RS12895"
                     /old_locus_tag="pgaptmp_002579"
     CDS             complement(32659..32877)
                     /locus_tag="ACHOSM_RS12895"
                     /old_locus_tag="pgaptmp_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU4559074.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWEL"
     gene            complement(32976..>33091)
                     /locus_tag="ACHOSM_RS12900"
                     /old_locus_tag="pgaptmp_002580"
     CDS             complement(32976..>33091)
                     /locus_tag="ACHOSM_RS12900"
                     /old_locus_tag="pgaptmp_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU4559075.1"
                     /translation="ICRSVLKKHRETVTLMGFTPTHLSLAIGMLNGVFKER"
BASE COUNT         8665 a         7682 c         8481 g         8263 t
ORIGIN      
        1 gagaatggag tgttcatgag aaactcacga ggtgctgaga tatgctctct atatgataag
       61 gatgcgttag tgcaacttgt tgaaactggt ggagctcatc ctctgagtcg agaacctata
      121 acagaatcaa tgatcatgag aaaagacgaa tgtcactttg attcaaaaaa agaagccttt
      181 gttgcaagtg atgcttaatt ttttctgttg gtgtgttttt atattaatag tttattataa
      241 tagtgccatg taaggatata ttgcctgaac aattattcag gcaatatttt ccttgcttta
      301 tatgaaatat ataatatttg gatccttaat ttctaaccaa ggggtcccat gtttttatgt
      361 tatgatgcag cccataattt cgggggctac atgcaagaat atctttttct tcggcgcctg
      421 atttgcgtaa aaacgtggct gcgccagaag aacaattacc ttgtgtttct gtgaatggcg
      481 gtaacatttt gtaagtcggt atgttgttga gcattgtttt tatattgtca gctggaattg
      541 atttttcttc atgtagtggt gtcgggataa gtgttccatt tctctgaagt acttcatctg
      601 aatataatcg atgtagcaac aaactctgtt ttgtctctat taatgagatt gagttgatat
      661 tgggtaagta acgaattgat aactgactga gttttattac atttcctgaa taaagatact
      721 cacctaactc tgaaaatttc cttcctgtta tcttatctgt atcatcagga ctaatatttt
      781 cgaacattct tacgtctcgc tctcctaagt ttggttttgc attatctttt ccattgtttt
      841 tatatatcca catctctttt ttttctgaag gaatcaaata tgtaagtgat tttacttcat
      901 ctgcaatttg tttttgttgg atattggtat tggcctttag cctagtttca ttatactttt
      961 tatgaaaatc ttcaatgcat gtaaattttt ggccttgtag ttttataacc gtttctttta
     1021 catcgattgg aaatttattt tgatttcgcg cattatttaa cttttttaaa aataaagagt
     1081 caatgtctat gcgggagcgt ttatcatcac tgtttatggc atagtattgg gctccgctcc
     1141 tgcttcccat tcctggagtc cctgcaataa taaatgagtg acttcttgta tgtctgccat
     1201 tgagtagagc aaggggagtt ttagctgtag ccaaccatac aggaacggat tttaaagata
     1261 taccatattc tttatgaaca attcttctgg agacggagtt cgtcattgtt gtttcatttg
     1321 aaagttgttg atgttcactg aacagatggt aacatgatga tacaactgat gatattttgg
     1381 gcataaataa tctcctcgct atatgtttgt tatatggtga ttaacttatt gtatgcttct
     1441 tatattgtgt ttttattttt aaatactgtt ttgttgaagg gggtatatgg gtaatgcgta
     1501 aaaacattgt tttgttatat taaaatgaca cttgtctttg cttataatca tttctggagc
     1561 aaaggggtca cggctgaggg ggtggagagc gttacgcagg ataagttcag tcaatgcttc
     1621 ctgatcatat aatgcacata tttctgaatt tcgagcattt ctgacaaaaa cacctgtctc
     1681 aggaatatgt agcgttatcg gacaattaag atgctcctca gtacaggaaa agtcttgtaa
     1741 actaacggga aatgcattcg aatttattcg atctagtagt gtttttatga cgctttcctg
     1801 cacaagcggg gcagcttgcc tctgctccag atataagctg tatgcctgca gaaaagtgtt
     1861 acctccgtta agttgtcttg ccaggctgtc tgcgagatga tgagactctc ttgccccaag
     1921 atggtcccaa agtccaccag gaataggctc tacactaaaa ccatccataa actggatata
     1981 atgaagcctc atattacggc ctcctatagt aatagtgtca ttctgattgc ttcttgcaat
     2041 atgtcgtaaa gcctccagct cattttccgg caatacactt ccattagaaa aatttaatat
     2101 gactggcata ttattatcct tttcaaccca ttttgtgaac tatattggta acaagagtca
     2161 acataccgct catgatatgt gaataaaaca atacaatttt taatagaata ttattgcatg
     2221 catgtttttt ctggctacaa taaacacaag tatgcaatat tgtaaacact tttttataaa
     2281 aaggaattat aatgccgatg aatactacag gtacgtcttt cagctctttt ggtataagtt
     2341 gccatagaga aaactccttc aggaactctt tccgagggaa gaatgatgag gtcataaaat
     2401 gttcaatggg agaacggacg attcgctttt ctgttcgcaa atttagcggc aatatactgg
     2461 atacagtgag taggcagagt actaaagata ttaatgggtg gataaaagat gaacggatag
     2521 tatatccctc aagggtgatc aaccaggaga ttgataatta ctgttttcaa aaaaatgcaa
     2581 aaatttccac tgaagagagg caaagggtct tttctcttgt gagtcaggaa tatcaactaa
     2641 ctcttgatgt taaggcagca caaagttcta ttaatcatgt gataatgggg aatgcttctt
     2701 ttggcaagaa aatagatgct ctttgtgata gtatgagccg ggatgtaaaa aatcgtacgg
     2761 cagatagcat agcaaacttg cttgcagaca agttttacca gaaacatatc gagccggata
     2821 ttgatattgt aaaactacga aatgagatcc cagattattt gaggtgtgct atacaggcat
     2881 aagtctctgg gagacgacta ggtcagtatc attggttttg ctctggtaaa aagagacgcg
     2941 gaaaaatgta caacaggcgc catacgtcat gcatggatta ggattgttca tttttatacc
     3001 atttgttgtt ttttatagcg atttgctaaa agggtgaaga aaaaacgttc tccaaaatta
     3061 gattgcaact gtttgatttt atggatagag gcatgcagtt tactcgacag taaaaagcat
     3121 gccaaatttg ctattaacct tttgaatata aaacactaat cgtctatttc tgttgccttc
     3181 ttattcagca gacgagaagc gcgccagtaa accagcaaca tcaggcccat tacgatagtc
     3241 agcgtaatgc tggtggctgc accaaacggc cagtcacgaa tattgaggaa ctggaccttg
     3301 atgacgttac cgatcagcag gtttttcgca ccgcccatca ggtcggatac atagaacagg
     3361 cccatcgctg gcagcatcac cagcagacat ccggcaataa ttcccggcat cgtcagtgga
     3421 ataatgatac ggataaaagt ctgtaacttg ctggcaccga gatcgcgcgc cgcctcaagc
     3481 aacggcttat ccagtttttc gatactggag tacagcggca tcaccataaa cggtagcaga
     3541 atgtaaacca gaccgataat caccgcactg ggcgtgaaca tgatacgaat tggtgtgtcg
     3601 ataacgccca gccagagcaa aaactcgttg agatagcctt tggtgctgag gaaaattttc
     3661 agcccgtaga tgcgaatcag tgagttggtc cagaagggaa caatcagcag aaacagcagc
     3721 agcggacgca ccttgtgtgg cagcttcgcc agaaaccagg caaacgggta gcccagcacc
     3781 aggcaggcga gggtggcgat cagcgccata ttcagcgagt gcaatagcac ttcaaaatag
     3841 agcggatcga gcagacgcgt gtagttatcc agcgtaaaga ccattttgac gaaactggcg
     3901 tcgtcgcggg tcaaaaagct ggtgccaatg atcatcaggt tgggcagaaa gacaaacaac
     3961 acaagccaac cgacaatagt gacaatcact acattctgga acttacttgt gttcttcatc
     4021 agccagtacg acctcccagc tttctaccca attaatggcc attttttggt cgagagagtg
     4081 gtcaaagtca gggtcgtctt cattgaagaa ttcgctgacc atcaccatct tgccattttc
     4141 cagttcaaca accgactcca gcgtcatgcc tttgtagtta cgctcacgaa cgtaaccaat
     4201 cagcccttca gcgtggttgt cgtcgttaat ctcttcaaca cgtaagtctt ccgggcgcag
     4261 cagaacatgc agtttttgcc ccggttcaac ggcgaagtta acgtagatat tacattcgcg
     4321 gccttcaacg ttggcgcgta cgcgctgctc gtcgagacgt tcgatgacag tggcgttaaa
     4381 catattgatt tcgccaatga agccggcaac aaacaggttt ttcggctctt cgtaaatttc
     4441 gcgcggtgta ccatcttgct caatacgacc ctcgcgcatc accacaatcc tgtctgacat
     4501 ggtgagcgct tcttcctgat cgtgagtgac aaagacgaag gtaataccaa gcttacgctg
     4561 taacgctttc agctcgttct gcatttgctt acgcagtttg taatccagcg ctgagagcga
     4621 ctcatccagc aacaacagac gaggcttgtt aaccaccgcg cgagcaatgg cgacgcgttg
     4681 ctgttgacca ccagagagct gatgcggttt acgttgagcg aaggtttcca gttgcaccat
     4741 ccgcagggct tccatcacgc ggggcgtaat ttcagcagcg ggggtttttt gcatgcgcaa
     4801 cccaaaggcc acattttcga acacggtcat gtgggggaaa agtgcgtagc tttggaaaac
     4861 agtgttcaca tagcggtttt ccgccggaac gtgggtgatg tcctcgttat ccagcatgat
     4921 gcgtccggaa tcaacagttt ccagacctgc aatcaggcga agaacggttg ttttaccgca
     4981 gccagaaggg ccaagcagcg tgaggaactc gccattgttg atagtcagat ccagctgggg
     5041 aatgacctct ttaccatcaa agcatttgcg aattcccgcc aattgcacca gcggtgaaag
     5101 cgaactcggt tgtttattca attttttact ctgtcccatg taaacgcaac ggatggctta
     5161 ccgatgcggg gtttgtggtt aaccaccttg gtgactctta atgagggcgg taattctacg
     5221 gcaaaccgct tgaatcgcca atctttgttg tgaattactg gcttagcttt atattcatta
     5281 aggtaatgct gataaatatt cccgcttgca ggggtaaaag tgacctgacg caatatttgt
     5341 cttttcttgc ttcttaataa tgttgtcaca aaaagtgagg gtgactacat ggataaacta
     5401 cttgagcgat ttttgaacta cgtgtctctg gatacccaat caaaagcagg ggtgagacag
     5461 gttcccagca cggaaggcca atggaagtta ttgcatctgc tgaaagagca gctcgaagaa
     5521 atggggctta tcaatgtgac cttaagtgag aagggcactt tgatggcgac gttaccggct
     5581 aacgtccctg gcgatatccc ggcgattggc tttatttctc atgtggatac ctcaccggat
     5641 tgcagcggca aaaatgttaa tccgcaaatt gttgaaaact atcgcggtgg cgatattgcg
     5701 ctgggtatcg gcgatgaagt tttatcaccg gttatgttcc cggtgctgca tcagctactg
     5761 ggtcagacgt tgattaccac cgatggtaaa accttgttag gtgccgatga caaagcaggt
     5821 attgcagaaa tcatgaccgc gctggcggta ttgcaacaga aaaacattcc gcatggtgat
     5881 attcgcgtcg cctttacccc ggatgaagaa gtgggcaaag gggcgaaaca ttttgatgtt
     5941 gatgccttcg atgcccgctg ggcttacact gttgacggtg gtggcgtagg cgaactggag
     6001 tttgaaaact tcaacgccgc atcggtcaat atcaaaattg tcggtaacaa tgttcatccg
     6061 ggcacggcga aaggagtgat ggtaaatgcg ctgtcgctgg cggcacgtat tcatgcggaa
     6121 gttccggcgg atgaaagccc ggaaatgaca gaaggctatg aaggtttcta tcacctggcg
     6181 agcatgaaag gcaccgttga acgggccgat atgcactaca tcatccgtga tttcgaccgt
     6241 aaacagtttg aagcgcgtaa acgtaaaatg atggagatcg ccaaaaaagt gggcaaaggg
     6301 ttacatcctg attgctacat tgaactggtg attgaagaca gttactacaa tatgcgcgag
     6361 aaagtggttg agcatccgca tattctcgat atcgcccagc aggcgatgcg tgactgcgat
     6421 attgaaccgg aactgaaacc gatccgcggt ggtaccgacg gcgcgcagtt gtcgtttatg
     6481 ggattaccgt gcccgaactt gttcactggc ggttacaact atcatggtaa gcatgagttt
     6541 gtgactctgg aaggtatgga aaaagcggtg caggtgatcg tccgtattgc cgagttaacg
     6601 gcgcaacgga agtaagcgaa aagggatgcg gcatgtgatg ccgcatccgg cttaaatcca
     6661 aacttaccct tcgaagaacc aatacccgct attgaccagc gccgcgagca tcgcgaggaa
     6721 tgacggatct tccagcgcat cgccaaaatt ctccgcagtc agcgcaatgt tgctggcgag
     6781 tgcatccagt accggacggt gcggggaatc gatcttctca ccattggcat acacgtcgtc
     6841 gccaatgcgc aatacgcgca gaccacccag gcgcaccagc acatcacctt gtttcagcgc
     6901 atcgtagatt tcatctggct gataaggcgg ttccggcggc gcgatatcca gttcatgacg
     6961 tgactgggat ataaactcgc caaaccattg cttaaagtgt tccggctggt tgatcaattc
     7021 gagcatcatc tcacgcagtt tatccatctc ttgaggcaga acatccgcag gatgagcgcg
     7081 aggtggaaca tcaggatcgc tgtagtagtt gccgcccagt tcacgttgca gcacataatc
     7141 ggcaaatcca ctaatcagtt cccgcgtatt tggcgcgcga aagcccacgg aatagttcat
     7201 cgcattttcc agcgcgtagc cttcatgcgg gaatcctggc ggaatataaa gaatatcacc
     7261 aggctccagc tcttcatcga tgatggcttc gaacggatcg acctgtaaca gatccggatg
     7321 tgggcagtgc tgtttcattt gcagcttttc gcccactcgc cagcgacgac gtccggtacc
     7381 ctgaatgata aacacgtcgt actgatcgag atgcgggccg acgccgccgc caggtacaga
     7441 aaaagaaatc atcagatcat caatacgcca gtccggtagt tcacggaacg gtcgcatcag
     7501 cgcggcggtc ggctcatgcc agtggttcac tgcctgtacc agtaatgacc agttggtttc
     7561 accgagatga tcgtagcttt cgaacgggcc gtggctgacc tgccatttgc catcctggtg
     7621 actgaccagt cgactgtcaa cttcgctttc catcgccaga cccgccaact cgtctggaga
     7681 gatcgggtca ataaaattat taaagccgcg ttttaacacc accgggcgtt tctgccagtg
     7741 acgttcaaga aaatcgggcc agttaagagt gagttggtat tccatgttaa gcatccacag
     7801 gctggtatct gcaaccgatt ataacggatg cttaacgtaa tgcgtgaagt atggacatat
     7861 tttatcaatt agtgtttttt gccgtttcat aatgtttcag gttgtgcatt atgtttttga
     7921 ttataaacag atttgtgatt tttcttgttc cagcttgttt cacgttgtag ccctttgtgc
     7981 aacgatcggt gtagttaatg gtgtagttaa ttgtgatgac cggattcaaa attatgagca
     8041 gatcacttaa caaactgagc gatacactgc taaggaaaat caacggcaca cccgcccaaa
     8101 aaacagcctt tcttaatgac ggtggaaacc tgagcgtcag gcattcaacc agtggccttt
     8161 taacctggta tttcacttac agggccggaa cgggaagggg ggcaccaccg gaacgcatta
     8221 agctgggaaa ttatcctgat ctgagcctga aatcagccag ggaaaaagcc gcccagtgtc
     8281 gcgcatggct ggctgagggg aaaaatccac gtcatgagat gaattacacc gtacaggaag
     8341 cgttaaagcc ggtaacggtt ggcgatgcgc tcacctactg gcttgagtcg tacgcaaagg
     8401 aaaaccgtgt ggattatgac ccactgaaaa agcggctaaa taatcacgta atacagcaca
     8461 ttggccctat gccgctggat aaatgcgagc tacggcactg gctggcctgt tttgatcggg
     8521 tggcaaagaa agcccctgtt tccgccgggt tcctgctaca gacatgcaag caggcgctta
     8581 agttctgcag gaagcggcgc tatgcaatca gcaacgttct tgatgatatg agtgtggcgg
     8641 acgttgggaa aaaaccggat ataagtgagc gtgtcttaag taacaaagaa cttggcgaat
     8701 tattgcaggc actggacaaa aaaatatttt ccccttacta cgtcgcacta atccgcctcc
     8761 tgattgtttt cggatgccgg acagtagaac tgaggttatc ggagatcggc gagtgggatt
     8821 ttaccgaaat gctctggacc gtgccgaaag aacacagcaa aacgaaggtg gcaatattcc
     8881 ggcccatacc ggaagcaata ctgccgttcg tcacgcagct ggtggagcag aacaggcaca
     8941 cgggcttatt gctgggggaa gtgaaacagg aaacaagcgt gtcgcagtac ggcagattag
     9001 cgcacaggag gctaaatcac cctcactggt cactacatga catccggcgc acctttacaa
     9061 cgatgctgaa cgatttaggc gtggatcctc acgtcgtgga gcagcttaca ggccaccaga
     9121 tgccaggaat gcagcgagtt tataatcatt cccgttatct ggatgctaaa cgcaatgcgc
     9181 tggatatgtg gacggagtgg ttagggatac tggcgggaac acatgaaaac gtaaccacgc
     9241 taccagtagc cagaagaaaa taattttttt cgtgtttttt cagtatgcgc atactggata
     9301 tgcgaacaga tacaacgtgc aacaatgagc aacaatgtgg aacaactacg aacaagaggc
     9361 gaaaaagcgt acggatttat aagtggctga tttttaatgt gttactggtt ttttatacac
     9421 tagcgaatca ctctttaaag agcgagaaaa aaggcatgaa acaatatttt atgcctttgt
     9481 attaacatga atttaaaata ttttattctt actgtttcat tttctgtaaa tcatacatcc
     9541 tcagtatttg ccattcatca ctctagcggg aacaatacga cacaataaga cacctgatga
     9601 ctctttaaga aacgaaaggg ggcaatagtg ttaagcactg atcggtttat acgtgaaaaa
     9661 gaatgcgaaa aactaaccgg ccttagccgt acgtgtcgct accgcctgga aaaggccgga
     9721 caattcccat cacgtcgtaa acttggcggt cgttccgttg gctggtcttt atccgaggtt
     9781 ctggcctgga aggatagctg caaggcagtt cattaatcac gctggcggca cgcagccgcc
     9841 acacatcaat catctgaaca cagagctata accatgaaga ttgaatatac gccagaacgt
     9901 gggcggggat tcgttcgccc tggtgaaact gaaaaacaac aaaattgggg tttttcaggc
     9961 ataaaaaaag cggccccgaa atggagccgc ctttctgaac agataacccg ctgcgccgtt
    10021 tgtgtgtgtg attccaaaca taagaacggc aatgatagcc gctatcaggc tggtgggcaa
    10081 tgcaatcagt ctggttcagt tcgttgccat acctgcaatg agcgcgtttc cctgtactct
    10141 ttaaggaatt gctcaagggc aaaagcacat ggcgcgaatc tttctgattc atgctctatc
    10201 tttctgcgcc gtcttttccg tgccggtgat aatgttttgg tcaattcttt atcggtcatt
    10261 gtgttgtcct gcatagcaat gcgccgtaat accttacacc acggcgctga tggtgattac
    10321 tctggttctt tggtcttgcg gcgctggagt tcttcacgtg cgacagtgac gagctgcccg
    10381 atctcctcgg cggctttgat tccgagtttt tccacctgcg ccagtgcatc gagcgaagaa
    10441 accaggggat tttctccact tccttctgcc tggcgccggg cgatctcacc gcgtacagcc
    10501 gtaactataa aattcgcttt agtttcccct ggctgtaggg atgcctcaat gccactcact
    10561 acttcatgtg gaaatctggc attcagttgc tgtgacttat tgttaacaga actcctggac
    10621 atgtttgcac ctcccattaa taacgggatg cagtgtaata caaaagtgaa taccaaaaaa
    10681 gacttgaaat ggtattcacc taaatgtaat gtgaatacca caagtgctga tacatgcaat
    10741 tcagcaattg aaacgacgaa gccccgcact gttggagcag taacggggct tctaaccacc
    10801 aacgataacg agagtatcga ggtagctatg agaaatcata ccacacaccc gcaagggcgg
    10861 gacccgcaca acctgaataa atacatctgg cgttttatcg ccctgagtac ggcacaaccg
    10921 cgcgtgatta ccattgaggc cagcagcgaa caggaagcac gccagcaatc cccggctggc
    10981 tgcgtgatgg tattcgccgc ccgtattcgt cagggggtgt tccatgcctg acatgaccaa
    11041 ttaccagtac ctgattaatc cgcattttaa ctgtgagcat gatattgcta aaaaggttta
    11101 ttccgctgct gatggggcta ctgacaatat atcaatggct gttgcctcaa ttggtaacct
    11161 gatgtggtat gcgtcagaaa atgaggaata tgacgaaaag gccatgcgca ttgacatggg
    11221 gaatattggc ttgttactgg caatgcttgg gcagtttgat atttcgttac ggtgcaccat
    11281 tgaaaatgcc gcagatgcat taaatgccat aaagaaagcg aatactgatt caaatcgggg
    11341 ataaataatc atgagaacat atttatctgg cttgactgcc agcggttatg cacaccccaa
    11401 aattatcccc ggcgctattt atctggataa gaacggtaac agagtaacgg taaaagaact
    11461 gatgtttgac cgtgtgtatt ttattcgtga tggctattca tttcatagtt cgctgaacgt
    11521 ggagatcttt attagcagat tccggcggga aatcccgact tccagaaata accatgtgtc
    11581 acgtgtggat gtggataaaa aactacagga actgaaaaac atgattgcct cgtggagaga
    11641 gcagaaatga aaaaagcgcc aaatttaaaa caccagccac gtgacaaaat gacggaagtc
    11701 atcatttttg cgggtagtga tgcgtgggca catgcgaagc agtggcagga acaggacggg
    11761 cgactggctg gcgataacgt gccacctgtc tggcttggag agcaacaact tgccgaactg
    11821 gacaacctgc aaatcgtacc ggacggacgc tatcgcgtgc gtctctacca ggcggggtta
    11881 ttgcgtcctg ggcttgttaa taccatcggg cagaaactgg cagcggcagg tgtcagggat
    11941 gctgattatt atcctgaagg aatgcacagc cagaaacggg agaactggcg cgaatatctg
    12001 gaacgtgagc ggggagagct ggcggaaaag aaaaaggtag ttgaactgcc tgtaaagaaa
    12061 aaagagcggg taaaagacga taacgcttca tcactggcgc ttaaccagat gggagcaagt
    12121 caacgcggcg aagttctcct ggcacattat ggcggtgaac tggcgattca tgccgactct
    12181 gacactgttc accattacaa cggcgttata tgggagccgg ttcaggataa agaattacag
    12241 cgagctatgg cacagatttt cattgatgcg gagatcagct attcgcagaa cgccattaaa
    12301 tcggcggtcg ataccatgaa gttaagtttg cctgtaatgg ggaatacagc ccgtaacctg
    12361 attggattca gtaacggggt atttgatacc agaacaggta attttcggga gcataacaaa
    12421 aacgactggt tgttaattgc cagtgaatta cctttcagcc caccagcaga gggggaaacg
    12481 ctggcaacac atgcgccgaa tttctggaag tggttacgcc gttcggtggc tgagaatgac
    12541 cgcaaggcgg atcgcgtact ggctgcatta ttcatggtgc tggcgaaccg gtacgactgg
    12601 cagttattca ttgaggtaac aggtccaggg ggaagtggta aaagcgtgat ggcggagatt
    12661 tgcaccatgc tggcgggtaa ggccaacaca gtatcggcaa gcatgaaggc gctggaagat
    12721 gcaagggaac gcgcgttagt ggttggcttt tcgctgatta tcatgccgga tatgacccgc
    12781 tacgctggtg atggggcagg gattaaggct attacaggcg gtgacaaggt ggcaattgac
    12841 ccgaaacaca aagcccccta ctcaacgcgt attccggcag tagtgctggc ggttaacaat
    12901 aacgccatgt cattcagtga ccgcagcggg gggatctcac gtcgtcgggt gatattcaat
    12961 ttttcggaag ttgtaccgga gaacgaacgc gatccaatgc tggcggaaaa aatagaaggt
    13021 gagctggcgg tagtgattcg ccatctgctt acacggtttg ctgaccagga cgaagccaga
    13081 cgcctgttat atgagcagca gaaatctgaa gaagcactgg cgataaagcg agagggggat
    13141 tcgctggtgg acttctgcgg ctatctcatg gcgtcggtaa tgtgtgatgg cctgttagtg
    13201 ggtaatgctg aaattgtacc attcagccca cgcaggtatc tctatcatgc ctatctggct
    13261 tatatgcggg cacatgggtt tggtaaacct gtaacactga cgcgcttcgg taaagatatg
    13321 ccgggggcaa tggcggaata tggcagggag tatatgaaac ggaaaacgaa gcacggtttg
    13381 cgttcaaacg tgacactgac ggaggaatca gaagactgga tgccatcatg tgtatcggtc
    13441 actaatgacg atagcaaaaa ttaaacttat ggaataactg ttcaccactg ttcaccctgt
    13501 cataaatatc ttttatatca gtatattata gggtgaacag ttatttatga actgttcacc
    13561 aaactattca ctgttcacct ttttgattgt ttattgagct tcaagggtga acagtggtga
    13621 acagttggtg aatagttttt gtgaaactgt tcacccatta acattatgaa ttaaaagata
    13681 aaatatcaaa aggtgaacag gtgaagggtt aaaacgcaaa aattttaatt tactgctgtg
    13741 agataaagcc tatgacagcg aagcacacaa aaaaatcaca atcgcacgcc cttgatttga
    13801 cggaacactg gttaagggtg tcgataaaaa tcatcgaccg caacgccggg gaaggatatg
    13861 cgaaagcaca tcccgaacta ataagcgcat tcatgaccac ggcggcggca aactttgcca
    13921 cgctgacgga acgggagatt gccgaagcgg aacaggtaac aaccatcaac gttaaaaccg
    13981 gagaggtgga atcatgacag cacagatagc cgcttacggg cggctggtgg acgacccgca
    14041 ggtaaaacag accagcaagg gcacaccgat gacgctggcg cgtatggcgg tcccccctcc
    14101 gtgcagccag gcagatgacg gaacggcgac gatgtggtta tccgtcctgg catttggcag
    14161 acaggccgaa gcactggcaa ggcaccgcaa gggtgaactc ctgagcgtgg cgggtaacat
    14221 gcagatcagc cagtggaccg gacagaacgg ggaaacactg cagggttatc aggttattgc
    14281 agacagcgta atcagtgccc gcgcggcacg tcctggcggg aacagacgca aaaccacagg
    14341 cacacagggt aatcagccac cagcgggagg cgatgaccct tacggtgacg gtattccgtt
    14401 ctgagggggt gacgatggta catgaccgca tagcggaaga actcgaggcg aaaggctttt
    14461 accggagggc ggcggcgcga tggggtgaag tcatgcagct ggtggagaca gacaaggaac
    14521 ggcatcacat cacgatgcga cggctggaat gttgcaggaa ggcacagagg ccaccggagc
    14581 caccggataa ttacggagac ctgaaaaagg cagtcgatcg cacttatgcc gaaatgggga
    14641 tagatggtgc tggtgatgaa atatggcgca attaccagga cagctaatca cacagccgga
    14701 gaaatccggc tttttttgca ccagttgaaa cggtatggcg cattaccggg ttttcgtcac
    14761 ggtctggcat agttactatc tgaaacaaac agacacaaca gaggaaaaaa caatgccgat
    14821 gaaatttgat gagatattaa aacagcgtga taaataccat gctgacaaca tggagacgat
    14881 gagcatcaat gattaccgcg cattcctgga gacgggcgca ctgattgaaa aggatcagca
    14941 tggttttgtt agatgtgccc tatccggtga aatgctggcg gtaaatcctg aacagataga
    15001 tgcaatgata gaatttctga aagggatcag ggactgagca cgcatacagc cggagcaatc
    15061 cggctttttg tcattttttg taaattattt gttcgtggtt gttccacgtt gttcactgac
    15121 aggatcggca tattttaccc gaactgaatc atgattattc tcgcccgtgg tgccaggacg
    15181 ctggggccat ttttcccgcc tgttaatgtg ttcaccaata ttcattcatt accaggcggg
    15241 aaagcaatcg gtgcgtttac tgatttcctt atgaaaaagc gttgagtttt tgccgcgtcc
    15301 tggagttcct tacttaaaaa tcagatgcgc gacatgctgg agaacgcgga aaaagaaaac
    15361 cgcagcctta acgatgcaga gggcgcaaaa tttgacgaat tacgcgctaa agctgaatcc
    15421 ctcgataaag acatttcccg ccttgaagcc attgcagacg aagagcgcag caagccaggt
    15481 aaaagcagcc agaccactga cccggcagaa ctccgcaact acatcctgac aggtgaaacc
    15541 cgcgcattaa gtacaggtgt ccccgctgat ggtggttata ccgttatccc tgaactgaac
    15601 accgaaatca tgcgaatgct ggcggatgag tccaccatgc gccgcatctg taccgtgaaa
    15661 aaaatcagca gcaacgagtt taagcagctt gtttccgctg gcggtgcggt cgttaaccac
    15721 ggtgaagagg gtaaggcacg cgaacagacc agcaccccgc agattaacga ggtgagcatt
    15781 aagctgtatc cggtctatgc gtacccgcgc accacacagg aaatcgtgga tttttccgat
    15841 gtggacatcc tttcatggct gacgggcgag attggcgaca ccttcacgga aaccgaagaa
    15901 agtgatctgg ttgtgggcga cggtgacaaa aaagcaaaag gttttttatc cgtaccccgt
    15961 gcagagaaga acgacaaaga acgtgatttc ggaacgttgc aggttatcaa gcccgttgat
    16021 gatcttgcct ggacatctgc ggacccgctg atcgatctga aatttgcatt acgtaaaaaa
    16081 taccgcaaga acgcggtatg ggtggttaac tccacgacgg cggcaaaact ccagaaggtg
    16141 aagaatgcga acggggatta tatatggcgt gaccgtttac aggcgggtga tcctgacaca
    16201 ttgctgggcc ttccggtcga atatctggag tttatgccgg ataacgttat tgccctgggc
    16261 gactttaaac gcggttacta catcgttgat cacgaaacag gtgttcgcac cagaccggac
    16321 aacctgacag aaccaggatt catcaaaatt ttcacgcaaa aatatttagg cggtggcgtg
    16381 gtggattcga acgcgatcaa gattctggaa ctgccacagg acgacgatta acagcataca
    16441 gaaggggcta aaaagcccct ttagtgtttt atgggtgaaa aaaattatga agagtatgga
    16501 aatccggtca tcggaaatca ccaccagcgc cagcaacacg cttacaggct acgttgttcg
    16561 ctgggataac ctttcggaac tgttatgggg ggagttttac gaaaaattcc agcggggggc
    16621 gtttactgag tggcttgcgg cgggtaatga cgttcgcggc ctgtatgagc atgaccacag
    16681 catgttactg gggcgcaccc gttccggcac gctgaaactg gaagaggacg acacagggtt
    16741 acgctttgaa cttaccccac cggataccag cacagggcgt gacgttatcg aactggttaa
    16801 acgtggtgat atatccggca tgagctttgg ctttcgttcc cgtaaggatg tatgggatac
    16861 cacaacagat ccatgcgtgc gcaccgtgct ggtggcggaa ctgtacgaaa ttaccgttac
    16921 atccgtaccg gcttaccccg attccggcgt ggagctggcc cgccgttccc tgtatgagca
    16981 gcaccccgaa aaaatgccgc gtgcggataa tcgccgctgg tgggcggatt tagcgggggt
    17041 gtgatatgtg gcctttcaga agaaaaaaag agcagcgcag catgacgctt gatgaattta
    17101 tggcgctggc tggcacatcg aacacggggg cgggtgagta cgtatcatcg gggacagcgg
    17161 aatcactccc cgccgtcatg aacgccgtca cggtcatctc tgaggcggtg gctactatgc
    17221 catgctacct gtacctggta cgcaatgaga aggggaagga ggcccgcgag tggcttgatt
    17281 ctcatccggt cgatcatatc ctcaacgagc gcccgaacgc atggcaaacc ccctaccagt
    17341 ttaagcgaat gatgatccgc cactgcctgt taaacggtaa tgcttacgcg gtgattcagt
    17401 gggggcgtga tggttttccg gcggctttac atccttaccc gccgcagtcg gtgaacgtgg
    17461 agcagacagg agaacacaac tggcgctatt gcatcactga cgcctacacc ggaaacaccc
    17521 gcaactattt accgtgggaa gtacttcacc ttcgttactc cacggatgac ggctttatgg
    17581 ggcgctcacc tgtaaccatc tgccgcgaat cgctggggct tgggctggcc caacaacgcc
    17641 acggcgcgag cgtgatgcgt gatggcatga tggcggcagg ggttatcacg tcaggcgaat
    17701 ggctggacgg cgtgaaaggc aaacaggcat tagccgcact ggaacgctat aaaggggcca
    17761 aaaacgccgg aaaaacgccc atccttgaag ggggcatgag ttaccagcag ctgggcatga
    17821 gtaaccagga cgccgaatgg ctggcctccc gtcgcttcac cattgaatac atcgcccgaa
    17881 tgttcaacgt ctcgccgatt tttctgcagg aatacagcaa cagcacctac agcaatttca
    17941 gcgaggcaag ccgcgcattt ctcaccatga cgatgcgccc gtggctggcg aactttgagc
    18001 agcagataaa aaacgccttg ctggtggtct cgcctgtacc tggtatccgg tatcaggtgg
    18061 agtttgacag cgaggacctg ttacgggcca cacctggcga acgctttgcc acctatgagc
    18121 gaggcatcaa atccggtgtt atgtgcccga acgaagcccg cgaacgagaa gggttgtctc
    18181 cgcgtgatgg tggtgatgag ttcagccagg catggaaaca ggaagtaaaa atcagcgagg
    18241 gagaaaaacc ggaatgaaca tagggcgact gcgtgacagg gtaacgattc agaccctgaa
    18301 acagaccaga gccatgacgg gcgaaatact cgaaacgtgg gaggacggtc acacactctg
    18361 ggcaagcgtg aacatggtca gcagcaagga ggccatttca tcgggtgcag agctggcgat
    18421 tggtaccgta aggatctgga tacggtaccg gaaggacatc aacgccacca gccggataaa
    18481 ggtcaatacg gggccgctgg cggggcgtgt actgaatatc atcgggcagc cgctgccgga
    18541 tgccgccagg acacgccttg aaattctttg tcgtgagggc gcggaaaaat gacagaagaa
    18601 cttatcaccc tagaagaagt gaaactccat tgccgcatcg atggcgacga ggaagaccag
    18661 ttaatcagcg gatacattgc cgcatcgctt gaggcgtgcc agatacacat aggcaggcgc
    18721 tttgatgacg ggctggagtt cacgccagcc ataaagattg gctgcatgat gtttatcgct
    18781 cacctgtatg agaatcgcca gattgtcgcg gataacgcaa aaacacacgt acccatgacg
    18841 attggcgcac tctggacggc ttaccgtgat gtgggggtgt actgatgccg tggcaaccat
    18901 taagacgatg tacagaacca ggctgcaaca ggcgcgtgaa gtccggcaag tgtgaggagc
    18961 acagacgcag cgcacgccag cagcaggaca gccggagagg cagcagcagg gagcgagggt
    19021 atacgaggca gtgggagaaa taccgcgcca tgtatctgag taaaaacccg ttatgtgcgc
    19081 attgcctgga aaaaggaata tacacgcccg ccgtggtggt ggatcacatt atcccaatcg
    19141 atggcggtaa tgatgttctc ttctggcctg agtggaatca tcagccattg tgccaggcgt
    19201 gtcataacca gaaaacgaaa tggcttgatc cgtccacaaa aaacaagcgt gctgcgggtg
    19261 gattccgtga ggaggaagaa cgggccgcta accgcaataa ctggatgtat ggcgctgatg
    19321 aatgagcggg aacaaaaccg ccttatccgt ggacttataa ggcagcgtga cgcatggaag
    19381 gcacaggaaa cagggcataa agataaagca tcaggacgcg cagaacgcat cacagcgacg
    19441 cgattaaacg accgtgaccg cgaggttatg gaatgtttcc gcaatcgctg atgaggcagc
    19501 cggacggggt ggggggagtt ttcaggacga acccgacccc gcccagaacc gaacgcctcc
    19561 tcaaattttt atgcacggga attttttgaa aaataatctg acgaaaaata agcatggcaa
    19621 gaccaccgaa agcccccgcc tacctggatg atatcgccgt gaagcagtgg cgggaaaaat
    19681 cgcggcagct tgcggagcgg ggagacctga cccccgccga ctggagcaat ctggaactgt
    19741 attgcgtcaa ctactccatt taccggaaag ccgttgcaga ccttgcggcg cgcgggttca
    19801 gcattgttaa cagtcagggc ggcgagagca gaaaccccgc attaagcgca aaatccgacg
    19861 ctgaaagggt gatgataaaa atggcctcct tgcttggttt tgacccgata agccgccgta
    19921 aaaatccacc ggaaacagaa gaagaggacg agcttgaccg cctggaataa gtacgcagaa
    19981 gacgtaaaaa cgggcaaaat tccggcctgt aaacggctga aacaggccgt taaacggtac
    20041 ttttcggacc ttgaaagccc cctttacacg ttcgatcgtg aggttgtgga gcggtttatt
    20101 gccttttcca gggtgtgccc gcacgtaaaa gggccgatgc gtggcagacc cattgagctg
    20161 gagccgtggc agcagttcgc ctttgcgtgc atcctcggct ttaaggttaa ggccaccgga
    20221 cggcgcaaat acacgagcgc ctttatcgaa gtgccgcgca aaaatgcgaa atccacgacc
    20281 gccgcgattc tggctaactg gtttctgatt atggagaacg gtcagcagga tatctacacc
    20341 gcagcggtga gccgtgacca ggcgcggatc gtctttgatg atgcgcgtca gatgtgcctt
    20401 ttatcccgac cgttacgcag gcgggtgaat attcaggcgc ataaggtgat acacccgaaa
    20461 agcaacagcc tgttaaagcc gctgacagga aaagcggcaa ccatcgaggg aacaaacccc
    20521 agcctttcca tcgtggacga ataccacttg caccccgata acggcgttta ttccgcgctt
    20581 gagctgggga tgggcgcacg tcccgaaggg ctgttatttg ccattaccac atcaggcagc
    20641 aacgtcgttt cagcctgtaa gcagcactat gattattgct gccagatcct ggacggcgaa
    20701 gaggtcaacg attcaatttt tgtactgatt tacgaactgg acgacgaaaa cgaggttgat
    20761 gatccggcga tgtggataaa ggctaaccct aacatcgatg tttccgtgga tcgtgaaaaa
    20821 ctggcctcaa ccatccagaa agcgcggggt attccgtcgc agtgggtgga gatgatgaca
    20881 aagcgattta atatctggtg tcaggggact accccgtgga tgggtaatgg tgcatgggct
    20941 gagtgtgccg gaacgttcac ggaggaagat ttacacggtc aggagtgtta cgcggggctg
    21001 gacttatcat caaccagcga tatttccagc gtgtgctatg cctttccggt cggtaaaaat
    21061 atcatgctgg tttcccgtca ttatctgccg gagttccagc tacagaaccc cgccaataaa
    21121 aaccgcgctg tctatcgcca gtgggctaaa gcgggctgga tacgcacaac gccaggtgac
    21181 tgcattgatt acgaccggat cagagatgac atcatgcagg acgcggagaa atttaatatc
    21241 aggctggtgg gctttgatac gtggaacgcc acgcatctca ggacgcaatt acagggggct
    21301 ggttttgagg tggagccgtt cccgcaaacc tacctcagat tcagtccggc ggcgaaatcg
    21361 ttcgaagttt ttgttaaccg cagggtgatt gtgcatcgtg gcgatccggt gttgtcctgg
    21421 tcgatgagta acgtcgtgat gcagagtgac gcgaatgcca atatcaagcc gaacaagaaa
    21481 aaatcaccga acaagataga cccgggcgtg gcggcactga tggcgtttgg tacattccag
    21541 gcagagcatg aggattttgc ttttgatata agcgacagcc accgccagaa actggaagaa
    21601 tttagcgggg tgtaatgagg tcagcagcat gacagaagcc gaaatactgc gattaatccg
    21661 ccgcgtctct ggaatcagcc agcaggctga cgaacagacc acgcagccgg acagcgtgac
    21721 agccgaaaat tatgcgcgtg ttgttgctga ggtgatgcgc cgtgatggta tccagcttaa
    21781 tgatgtggat atgcgcgaca tacggatccg cgttctggaa atgctggcct atcgtcgccg
    21841 cgtggagatg tatcgggaga aggaaaaaat aacgtaccac tggaagaagc cggagcgctt
    21901 gcggcggtaa ctggttgata ttcccgacgg cccaaaaatg gggggctaca tccctcgctt
    21961 ctgaggactg ttaacaagct gcctgaaaga cagtcacaaa ctacgaaatt ttcgcagttt
    22021 aattaatggt cagcttaatc gctaacccgc tgatattttc ggaaacctca atttgaggaa
    22081 gtcggcgcgg taactcgctg aacttaaagc aaagctcaaa attgagctgg ctgaataatc
    22141 attatgatta cgcagatgat taaggaatga ccgaaggcgg aaattcgcct gtggttgatg
    22201 ggggagttgc agatctgcaa ctcgaccatg aaactacgga aactacccgt agtttgggta
    22261 gtaagagtaa cacccagatt ttggggctta ctcgtgatac ccaaatttgg ggtatcggtg
    22321 gcaaccataa cgactttcgt tacggttgat gcttttaccc cattggggga aaggtattac
    22381 gataatcata acacctaccg aacaggtaaa acccaccagc ctgattaaca gttaaccgga
    22441 aaaaaaacca ggtggccccg ttatggggat ccccatatcg acattaacgc ccctcatgaa
    22501 ttggtgtgct tccccctgga aagattaccc gcctgtatat ttcttgtgtc tatttgttcc
    22561 acgttgtttc atacagtgca ccgaacggtg tagttaatgg tgtagttaat ttgctgtttt
    22621 tagcactttt tgtagtgtga gttttttaat taaattaatg ggttactttg ctgtggtgat
    22681 aaaaggacat atttattcat ctttcggcgc agaatgctgg cgaccaaaaa tcacctccat
    22741 ccgcgcaccg cccagcatgc tctctccggc gacgatttta ccctcatatt gctcggtgat
    22801 ttcgcgggct accgccagcc ctacaccttg cccagggcgt aaagtatcaa cccgttgacc
    22861 acggtcgaaa atgacctctc gcttgcttaa tggaataccg gggccatcat cctcgaccac
    22921 aatatagaga tgctcgtcgg tttgccttgc agaaatttcg acaaactcga ggcaatattt
    22981 acaggcatta tccagcacgt tgcccatcac ctcgacaaaa tcgttctgct caccgacaaa
    23041 gctgatctct ggcgaaatat cgagagagat attgacccct ttgcgttgat acactttgtt
    23101 cagcgctgag gtgagattgt ccagcagtgg ggcgaccgga tgcagctcgc ggctgagcaa
    23161 tgtcccgccg cgcatactgg cacgatgcag gtagtagcca atttgctgtg aaatgcggct
    23221 gatttgctcc agcattaccg gctcagcatc actgacgctc atcttttcac tacgcagaga
    23281 acgcagcgta ctttgcagca ccgccagtgg cgttttcaga ctatgggtca ggtcggtgag
    23341 cgtcgtacgg tatttgtcgt aacgttcgcg ttcacttttt aacaatcggt tcaggtttcg
    23401 taccagactg gtcagttctc gcgttgtggc tggattgagc aattcgcggt tatgttcttc
    23461 cagttcgcgg acttcttttg ccagggcttc gatggggcgt aaactccacc aggcggcgac
    23521 ccacagcagc gggatcacta acagcagatt ggctgagagc acatagataa accagctcca
    23581 gaccatatag gaacttttta gctccaccgg aatggtatcc accaccacaa tggttaattt
    23641 tggcatccgc gatgttgccg ggtagacgtt taccgccacc gagtgggtca tctccgcgtc
    23701 gtcatcatct tcccgcactt cctgcaactg ttgctgtatc gaatgatctc cactcagcaa
    23761 gaggctggta tcgttaacat ccgcttcaat ttcatgaaaa ccattcgatt tcagccagtc
    23821 aggctggatc atcttcatca gccagggcac gtcacgttgc gcccataaaa gctgcccgtt
    23881 ctcatcataa attagcgtca tggtggggct ttgcttgtcg atattttcgg gtaactcgac
    23941 atgcaactta ttgttttccc acttcgcaag ggtatagaac agattgctct cgccacgtaa
    24001 cagccgaaac gtagttttat cgaaactgac gctataaccg atcagcgcga ccattccgta
    24061 ggcaagcgaa agcaccagta ctaccgctgc cgttgccaac agaaaacgta cccgcagcga
    24121 gagcgggaaa aaaagacgca gtaatttttt catcagcgca attcgaacag gtagccctgg
    24181 ccgcgaacgg tggtaatgac ttcttgcgga tattgtgcct gaattttttt gcgcagacgt
    24241 cccatcagta catcaatggt atggctttcc cgcagctccg catccggata gagttgtagc
    24301 attaacgaat ctttgctgac cactttgcca ttattgcgta tcaacgtttc cataatggtg
    24361 tattcgaacg cggtcagttt gatcacttcg tcattaatag ataattcacg gcgagagaga
    24421 tcaacctgaa acggggggag cgaaatgacc tgtgaagcca gaccgctatt acgccgcatt
    24481 aatgcctgca ttcgcgccat cacctcttca atatgaaacg gtttagtcac gtaatcatca
    24541 gcaccggcac ttaatacttc gactttgtcc tgccagcttt cacgggcggt taataccaga
    24601 atcggcagtg aaacatcgtt gctacgccag cggcgaatca gtgacagacc gtcctcgtca
    24661 ggcaatccga gatcgacaat cgcaatatcc ggtaaatgtt cattgagata ataatcggct
    24721 tctttggcat cttctgcgtc atcgacctga tgaccagcat cctgaatctg aactttaagg
    24781 tggtgacgta acaacgcatt gtcttcaaca accagtacgc gcatttttat ttctccctgt
    24841 cttaattatt aaaatagtgt aacgcgatta tgtgtttatg ggggtaaaca ttaaataaac
    24901 cagcggggag gggaggtaaa gtgaaaaaag aaaaagtgga taatcttaat aagcaggccg
    24961 gacagcatcg ccatccggca ctgatttgag gcttatttca gctcatcaac catcgtgata
    25021 gctcgaccaa tatagttagc cggcgtcatc gctttcaggc gggctttctc ttcttctggc
    25081 aacgccagac catcgataaa ctgcttcatg ccttcggcgt caacgcgctt accgcgagta
    25141 agctctttca gcttctcgta cggtttttcg atgccatagc gacgcataac tgtctggatt
    25201 ggttctgcca gcacttccca gttgtgatcc agctcatcca gcagatggtc acggttcact
    25261 tccagtttgc tcacgccttt cagggtggat tgatacgcaa tcagcgcata accgataccc
    25321 acgccgaggt tacgcagcac ggtggagtcg gtcaggtcac gctgccagcg ggaaaccggc
    25381 agtttgcttg ccagatgctg caataccgcg ttggaaaggc ccagattccc ttcggagttt
    25441 tcgaagtcga tcgggttaac tttatgcggc atggtggaag aaccaatctc accagcaatg
    25501 gttttctgtt tgaagtggtt aagggcgata taaccccaga cgtcacggtc aaagtcgatc
    25561 agaatggtgt tgaagcgcgc aacgcaatca aacagttcgg caatgtagtc gtgcggttca
    25621 atctgggtag tgtacgggtt ccactgaata cccagcgagg tgacgaactc ttcgctgaac
    25681 tgatgccagt caacttccgg gtaagcggcg atgtgggcgt tatagttacc gaccgcaccg
    25741 ttgattttgc cgaggatctc cacctggtta agctggcggt actggcgctc catacggtag
    25801 gcgacgtttg ccatctcttt accgatggtt gacggcgtgg ctggctgacc gtgggtacga
    25861 gacagcagcg ggatatcgcg gtactgaacg gcgagatctt taatgccatc aatcagttga
    25921 cgccagtacg gcaggatcac ttcatcacgc gcggttttca gcattaatgc gtgggagagg
    25981 ttattgatat cttccgaagt acaggcaaag tggatgaatt cagaaaccgc gtgcagttcc
    26041 gggatctccg ccactttttc tttcaggaaa tactcaaccg ctttaacgtc gtggttagtg
    26101 gtacgctcga tggttttgat gcgtgcggca tcttcttcgc tgaaattggc gacaattgca
    26161 tcaaggtaac cgattgcgtc ggcagcaaaa gcaggaactt ccttgatcgc tgcgtgcgcg
    26221 gccagttttt gcagccaacg tacttcaact tgtacacgga atttcagcaa accatattcg
    26281 ctgaaaatcc cgcgcagcgc gctgacttta tcgccgtagc gtccatcgac aggggaaacg
    26341 gcggtcagtg aggataattc catagatcac aactccgggg ttaaatgagc aagaatttgt
    26401 tttgcctgag tggtcaggcg attacgagaa aacatcagtt gcagacgtcc gccgccgacc
    26461 tggtgccaga gcacggcggc gcgaatgcct gccagcaggg ttgcgcgaac tttcgcctgc
    26521 acttgtgggc tttgcagtac agcaggggaa ccggtgacct gaatgcgcgg gccaagcggg
    26581 ctgatcacat caacatagat agcagccatc gcgctcatca gcgtttcgga ctgtaaatcg
    26641 aagtgttcga gctggcgttg caggccgttg atccggttgc ccagagtgtc gagcgcgcct
    26701 ttcgctgagg agagtttgcg ctcaagcacc atcaagctga gtgtgtagcg ggttaattcg
    26761 gcgtttaagc cctggcgact gctggcattg agcacgccga gcagggtttc cagcccgacg
    26821 cgcaggttgg cttcgctacc gccaaaaacc gccagcgtcg agctggggtt catatcaata
    26881 atactgttga gtgagacgtg tagcgcatcg gcatcacaat gcccctggtg agcgagttgt
    26941 tgcaccaggc gtgccgactg acaaataccg gccagggcga gggtgatgtc atagtaattc
    27001 tttgccacgt tcactgcttc cttgttttaa gtaaagataa taatcagacc ggcagcggca
    27061 gacgctgctc aataataccg ccaccgaggc acacttcacc gttatagaag acggcagact
    27121 ggcccggcgt cacggcggca accggttcat cgaaaatcac ttcaatgcga tcatcgtcca
    27181 gcgccttgac ggtgcaaggg atatcggtct ggcgatagcg ggtttttacc gtgcaacgca
    27241 tagtgccggt gaatggttcg cgatcgaccc agtgcaactg ctgggcaatc aacccgacag
    27301 acatcagccg cgggtgttca tggccctgag cgacaaccag aatgttgttt tcgacgtctt
    27361 tgtccaccac ataccacggt tcttcggtgc cctctttggt gccaccgata cccagacctt
    27421 tacgctgacc gagagtgtga tacatcagcc cctggtgctc accaatttca tcgccatcga
    27481 cggtaatgat tttgcccggt tgcgccggga gataacggcc caggaactcg cggaatttac
    27541 gttcgccaat gaagcaaatg ccggtagagt cttttttctt cgcggtgacc agaccaagat
    27601 cttcagcaat cttacgcacc tgcggttttt ccagttcgcc gaccgggaac aggctttgcg
    27661 caatctgctc atggctgagc gtataaagga agtagctctg gtctttattg ctgtccagac
    27721 cacgcagcag gcggctcttg ccatcgacat cggcccgacg tacgtaatga ccggtagcga
    27781 tataatcggc acctaaatct tcggcggcaa attcgaggaa agctttaaat ttgatctctt
    27841 tgttgcacag aatatccgga ttcggcgtgc gaccggcttt atattcggca aggaacagtt
    27901 cgaagacatt gtcccagtac tcggcagcaa agttaacggt gtgcagttca atgccgagct
    27961 tgtcgcagac agcctgggca tcagccagat ccgctgccgc tgtgcaatat tcctcaccgt
    28021 cgtcttcttc ccagttcttc ataaacaggc cttcaacctg atatccctgt tgttgcaaca
    28081 gccaggcaga aacggaggaa tcgacaccgc cggacatgcc gacgattact ttttttgcgg
    28141 tttcagacat tggatcactc acgacattga acttcaaggc ggcgtattct atcacgcagc
    28201 cttttagatg acaccctttg taaaaggcca gttaaaatcg ccaatcatct ccagcggata
    28261 gcgttgcccg ctttgataac aacggatact ttccgccacc agcggcgaac gaagatttga
    28321 cgcctttaaa atttcttcgg cgctgaccca acgacagcag tcgatatcgc tgtcatgagg
    28381 ctgggtcggg catatttgct caagctcaat ggcaaagagg aaacgcaaaa acggcgtttt
    28441 atctggcgca atccactgat gcatacgaat aaagtgttgc ggttgcgcgc tgatgccggt
    28501 ttcttcccat agctcacggg cggcagcttc cactaaggtt tcatcggctt ccagatgccc
    28561 ggcaggttgg ttccataacg ctttaccatt aatcgtctct tcaacgacta aaaatttacc
    28621 ctctgcgtgc actacgcaag caacggtaac gtgcggttta aacattgtgc atccttattc
    28681 tgttgcttct cgccattcgc cattggcaag gttatccaaa gtgtaatcac ccatcgcata
    28741 acgaatcagt cgcagcgtgg ggaaaccaac atgggcggtc atgcggcgca cctggcgatt
    28801 acgtccttca tataaggtga tcttcagcca gctggtggga atacttttgc gttcacgaat
    28861 tggtggattc cgcggccata accacgctgg ttcgtcaacc agctccgcgc cggcgggcag
    28921 ggtagggcca tcatttaagg ttacgccatt gcgcaaggct tcaagtgcgt cttgtgtggg
    28981 aataccttcc acctgcacat aatagatttt cccggtgcgt ttgcccggct gggttaaacg
    29041 cgcctgcaac gcgccgttat tggtcagcac cagtaaccct tcgctatcgc ggtcaagacg
    29101 acctgctgca taaacgccct gaaccgggat gaattctttt aatgttttgc gtccggcttc
    29161 atcggtgaac tgcggaagaa catcgtaggg tttattgaac aggatcacac gcgtgggctg
    29221 gttttcaggt ttacgcctgg tagaacgttg cgagctgaat cgcttaacct ggtgatttct
    29281 aaaagaagtt ttttgcatgg tattttcaga gattatgaat tgccgcatta tagcctaata
    29341 acgcgcatct ttcatgacgg caaacaatag ggtagtattg acaagccaat tacaaatcat
    29401 taacaaaaaa ttgctctaaa gcatccgtat cgcaggacgc aaacgcatat gcaacgtggt
    29461 ggcagacgag caaaccagta gcgctcgaag gagaggtgaa tggaaagtaa agtagttgtt
    29521 ccggcacaag gcaagaagat caccctgcaa aacggcaaac tcaacgttcc tgaaaatccg
    29581 attatccctt acattgaagg tgatggaatc ggtgtagatg taaccccagc catgctgaaa
    29641 gtggtcgacg ctgcagtcga gaaagcctat aaaggcgagc gtaaaatctc ctggatggaa
    29701 atttacaccg gtgaaaaatc cacacaggtt tatggtcagg atgtctggct gcctgctgaa
    29761 acccttgatc tgattcgtga atatcgcgtt gccattaaag gtccgctgac cactcctgtt
    29821 ggtggcggta ttcgctctct gaacgttgcc ctgcgccagg aactggatct ctacatctgc
    29881 ctgcgtccgg tacgttacta tcagggcact ccaagcccgg ttaaacaccc tgaactgacc
    29941 gatatggtta tcttccgtga aaactcggaa gacatttatg cgggtatcga atggaaagca
    30001 gactctgccg acgccgagaa agtgattaaa ttcctgcgtg aagagatggg ggtgaagaaa
    30061 attcgcttcc cggaacattg tggtatcggt attaagccgt gttcggaaga aggcaccaaa
    30121 cgtctggttc gtgcagcgat cgaatacgca attgctaacg atcgtgactc tgtgactctg
    30181 gtgcacaaag gcaacatcat gaagttcacc gaaggcgcgt ttaaagactg gggctaccag
    30241 ctggcgcgtg aagagtttgg cggtgaactg atcgacggcg gcccgtggct gaaagttaaa
    30301 aacccgaaca ccggcaaaga gatcgtcatt aaagacgtga ttgctgatgc attcctgcaa
    30361 cagatcctgc tgcgtccggc tgaatatgat gttatcgcct gtatgaacct gaacggtgac
    30421 tacatttctg acgctctggc agcgcaggtt ggcggtatcg gtatcgcccc tggagcaaac
    30481 atcggtgacg aatgcgccct gtttgaagcc acccacggta ctgcgccgaa atacgccggt
    30541 caggacaaag taaaccctgg ctctattatt ctctccgctg agatgatgtt acgccatatg
    30601 ggttggactg aagcggctga cctgattgtt aaaggtatgg aaggcgcaat caatgccaag
    30661 accgtaacct atgacttcga acgtctgatg gaaggcgcta aactgctgaa atgttcagag
    30721 tttggtgacg cgatcatcaa gaatatgtaa tcactacatg tgttaaatat tgaaacgggc
    30781 gtataacacg cccgttgttt tatgatgatt taaaatcttc cccaaaactt tccccaaaac
    30841 ccttccccaa aactggctat tttctatgct gttttgatat ctacgataat ccagtcttta
    30901 ccacgatcat cattgtatcg gtcggtcatt tttctggatt tatgacctaa caacttttgc
    30961 gtatccagac cctgttcccg atataaccgt tctgacagag atcgctgctc atgaaatgtg
    31021 ggcgcagttc cttgctccca ttttatgcca catttttccc tggccttttt aaaagccgtt
    31081 gtaagagtat ttgcagacac ctggtctcct ctgtttgctt gagaggtagt gtgacggtaa
    31141 tggaccagat atttactaac aacagcatcc ctgcactgag atataacttc acgaagggta
    31201 atattcagag catcgcattt caggttaagc ggaatagcaa gttttgaacc ggttttttcc
    31261 tgagtaatgt gcaacatgtc gtcccagata tcagagaatt tcaaattgca gatatcgcct
    31321 aaacgttgtc cagtgacaag agcaagtagc atcccgcatt ttaaataggg ctgccgtctg
    31381 cttacgctgt caaatattgc ctgccattcg gacagtgata atctttggcg gtttactcga
    31441 tttcgcggtt gttttgttgc ctgcgctggg ttaaatcctg gcggaacatg tcctgcgtgt
    31501 tgtgcttctt tgaagacgtc gatcaacacc attctcacga cttgcgccat cctgttatga
    31561 ccttcagcct ttacagcatc aattatttcg gcaatatcaa gtgcggtaat atccttgagg
    31621 tgttgcattc cacaatgctc acggaagaga cgaatgggtt tgcctttttg ccgataggag
    31681 ttgggtctta gttcattatg ttgcagcctg tcctcctgga tagaaatata tttatcaagc
    31741 cattctgtca ccgtaatgtc tgagcgcctg cctttcattc tttccagacg ctcattgacg
    31801 cttaatattt gtcgggtacg ttgttcagca ataatggtat ttgcttcagt agcaacctgt
    31861 tttgcttcat tctcatcagt tcctaaacta tgaaaacgac cggatagtgg atgtttgtat
    31921 tgccaatata cctttccggt tcgcttatct aatttgcaat ataaattggg tatagagatt
    31981 ttgtgagatc ggggtctagc agccatcagc gattatccgt tggagttttg ggtttgcgtt
    32041 gattgggagt tgcggttctg caagcgttcc tacaaaacgg gaatttcggt caatcatcca
    32101 gtagcgacca acttttatag cgggtggggc catcattttc ccttgcgcgt attttttcag
    32161 aactcgctca cttggtgcta agtccccaaa ttcttcttta gcccagtcct gtaaagtgat
    32221 tagtcgagac atttgtcctc ctcttagctg ctgagggagt ttgtgaccga tatatctgac
    32281 atgatattaa gcttatggca ggtacatctc ttgactggtc atagagataa atttaatgct
    32341 gagaaatgca gtattgaatt tatcaatttt tctatttcct gcgtatggca cgtaacttct
    32401 taatgtgttc tgccgtttcg atctcttctg ctatccgatc tgcatcagct ttattcacag
    32461 gttcaaagtc atgattaaag cggaacatgc tggcgataca tgttctgcct tttcggatgt
    32521 agtgaacttt gttgtgggta gaacgcagga ttttgcaggg agtgccgtgg tgatcgacgt
    32581 accaggtgtt aggaaaaatg attctgaaca tttttacacc tcaattggac gatgttgaaa
    32641 tttgctgctt tgaggccatc acagttccca ttgtttgttc ttaagttcga tctcctcctg
    32701 gcaacttgca caagtccgac aaccctgaac ggccaggcgt cttcgttcat ctatcggatc
    32761 gccacactca caacaatgag tggcagatat agcctggtgg ttcaggcggc gcatttttat
    32821 tgctgtgttg cgctgtaatt cttcaatttc tgatgctgaa tcaatgatgt ctgccatctt
    32881 tcattaatcc ctgaattgtt ggttaatacg cttgagggtg aatgcgaata ataaaaaagg
    32941 agcctgtagc tccatgatga ttttgttttt catgttcacc gttccttaaa gacgccgttt
    33001 aacatgccga tcgccaggct taaatgagtc ggtgtgaatc ccatcagcgt taccgtttcg
    33061 cggtgcttct tcagtacgct acggcaaatg t
//
LOCUS       DAZKHC020000048        32413 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZKHC020000048 DAZKHC020000000
VERSION     DAZKHC020000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.048"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(65..1825)
                     /gene="ycaO"
                     /locus_tag="ACHOSM_RS12905"
                     /old_locus_tag="pgaptmp_002581"
     CDS             complement(65..1825)
                     /gene="ycaO"
                     /locus_tag="ACHOSM_RS12905"
                     /old_locus_tag="pgaptmp_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HGU4559076.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1955..2647
                     /locus_tag="ACHOSM_RS12910"
                     /old_locus_tag="pgaptmp_002582"
     CDS             1955..2647
                     /locus_tag="ACHOSM_RS12910"
                     /old_locus_tag="pgaptmp_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGU4559077.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLSGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2846..3934
                     /gene="serC"
                     /locus_tag="ACHOSM_RS12915"
                     /old_locus_tag="pgaptmp_002583"
     CDS             2846..3934
                     /gene="serC"
                     /locus_tag="ACHOSM_RS12915"
                     /old_locus_tag="pgaptmp_002583"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HGU4559078.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAIKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4005..5288
                     /gene="aroA"
                     /locus_tag="ACHOSM_RS12920"
                     /old_locus_tag="pgaptmp_002584"
     CDS             4005..5288
                     /gene="aroA"
                     /locus_tag="ACHOSM_RS12920"
                     /old_locus_tag="pgaptmp_002584"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGU4559079.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5457..6221
                     /gene="ycaL"
                     /locus_tag="ACHOSM_RS12925"
                     /old_locus_tag="pgaptmp_002585"
     CDS             5457..6221
                     /gene="ycaL"
                     /locus_tag="ACHOSM_RS12925"
                     /old_locus_tag="pgaptmp_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HGU4559080.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVVSKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6394..7077
                     /gene="cmk"
                     /locus_tag="ACHOSM_RS12930"
                     /old_locus_tag="pgaptmp_002586"
     CDS             6394..7077
                     /gene="cmk"
                     /locus_tag="ACHOSM_RS12930"
                     /old_locus_tag="pgaptmp_002586"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGU4559081.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7188..8861
                     /gene="rpsA"
                     /locus_tag="ACHOSM_RS12935"
                     /old_locus_tag="pgaptmp_002587"
     CDS             7188..8861
                     /gene="rpsA"
                     /locus_tag="ACHOSM_RS12935"
                     /old_locus_tag="pgaptmp_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGU4559082.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9021..9305
                     /gene="ihfB"
                     /locus_tag="ACHOSM_RS12940"
                     /old_locus_tag="pgaptmp_002588"
     CDS             9021..9305
                     /gene="ihfB"
                     /locus_tag="ACHOSM_RS12940"
                     /old_locus_tag="pgaptmp_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HGU4559083.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9512..11776
                     /locus_tag="ACHOSM_RS12945"
                     /old_locus_tag="pgaptmp_002589"
     CDS             9512..11776
                     /locus_tag="ACHOSM_RS12945"
                     /old_locus_tag="pgaptmp_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HGU4559084.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11813..13561
                     /gene="msbA"
                     /locus_tag="ACHOSM_RS12950"
                     /old_locus_tag="pgaptmp_002590"
     CDS             11813..13561
                     /gene="msbA"
                     /locus_tag="ACHOSM_RS12950"
                     /old_locus_tag="pgaptmp_002590"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HGU4559085.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13558..14544
                     /gene="lpxK"
                     /locus_tag="ACHOSM_RS12955"
                     /old_locus_tag="pgaptmp_002591"
     CDS             13558..14544
                     /gene="lpxK"
                     /locus_tag="ACHOSM_RS12955"
                     /old_locus_tag="pgaptmp_002591"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HGU4559086.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14581..15813
                     /gene="ycaQ"
                     /locus_tag="ACHOSM_RS12960"
                     /old_locus_tag="pgaptmp_002592"
     CDS             14581..15813
                     /gene="ycaQ"
                     /locus_tag="ACHOSM_RS12960"
                     /old_locus_tag="pgaptmp_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HGU4559087.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            15865..16047
                     /gene="ycaR"
                     /locus_tag="ACHOSM_RS12965"
                     /old_locus_tag="pgaptmp_002593"
     CDS             15865..16047
                     /gene="ycaR"
                     /locus_tag="ACHOSM_RS12965"
                     /old_locus_tag="pgaptmp_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HGU4559088.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16044..16790
                     /gene="kdsB"
                     /locus_tag="ACHOSM_RS12970"
                     /old_locus_tag="pgaptmp_002594"
     CDS             16044..16790
                     /gene="kdsB"
                     /locus_tag="ACHOSM_RS12970"
                     /old_locus_tag="pgaptmp_002594"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HGU4559089.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            16944..17837
                     /locus_tag="ACHOSM_RS12975"
                     /old_locus_tag="pgaptmp_002595"
     CDS             16944..17837
                     /locus_tag="ACHOSM_RS12975"
                     /old_locus_tag="pgaptmp_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HGU4559090.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17814..18593)
                     /gene="elyC"
                     /locus_tag="ACHOSM_RS12980"
                     /old_locus_tag="pgaptmp_002596"
     CDS             complement(17814..18593)
                     /gene="elyC"
                     /locus_tag="ACHOSM_RS12980"
                     /old_locus_tag="pgaptmp_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HGU4559091.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            18729..19514
                     /gene="cmoM"
                     /locus_tag="ACHOSM_RS12985"
                     /old_locus_tag="pgaptmp_002597"
     CDS             18729..19514
                     /gene="cmoM"
                     /locus_tag="ACHOSM_RS12985"
                     /old_locus_tag="pgaptmp_002597"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HGU4559092.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19511..20833
                     /gene="mukF"
                     /locus_tag="ACHOSM_RS12990"
                     /old_locus_tag="pgaptmp_002598"
     CDS             19511..20833
                     /gene="mukF"
                     /locus_tag="ACHOSM_RS12990"
                     /old_locus_tag="pgaptmp_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HGU4559093.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20814..21518
                     /gene="mukE"
                     /locus_tag="ACHOSM_RS12995"
                     /old_locus_tag="pgaptmp_002599"
     CDS             20814..21518
                     /gene="mukE"
                     /locus_tag="ACHOSM_RS12995"
                     /old_locus_tag="pgaptmp_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HGU4559094.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21518..25978
                     /gene="mukB"
                     /locus_tag="ACHOSM_RS13000"
                     /old_locus_tag="pgaptmp_002600"
     CDS             21518..25978
                     /gene="mukB"
                     /locus_tag="ACHOSM_RS13000"
                     /old_locus_tag="pgaptmp_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HGU4559095.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAESFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26239..28086
                     /gene="ldtD"
                     /locus_tag="ACHOSM_RS13005"
                     /old_locus_tag="pgaptmp_002601"
     CDS             26239..28086
                     /gene="ldtD"
                     /locus_tag="ACHOSM_RS13005"
                     /old_locus_tag="pgaptmp_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4559096.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAELAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGLKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28267..28815
                     /locus_tag="ACHOSM_RS13010"
                     /old_locus_tag="pgaptmp_002602"
     CDS             28267..28815
                     /locus_tag="ACHOSM_RS13010"
                     /old_locus_tag="pgaptmp_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HGU4559097.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILILNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28842..29489
                     /gene="gloC"
                     /locus_tag="ACHOSM_RS13015"
                     /old_locus_tag="pgaptmp_002603"
     CDS             28842..29489
                     /gene="gloC"
                     /locus_tag="ACHOSM_RS13015"
                     /old_locus_tag="pgaptmp_002603"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HGU4559098.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29711..30901)
                     /gene="aspC"
                     /locus_tag="ACHOSM_RS13020"
                     /old_locus_tag="pgaptmp_002604"
     CDS             complement(29711..30901)
                     /gene="aspC"
                     /locus_tag="ACHOSM_RS13020"
                     /old_locus_tag="pgaptmp_002604"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HGU4559099.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31086..32174)
                     /gene="ompF"
                     /locus_tag="ACHOSM_RS13025"
                     /old_locus_tag="pgaptmp_002605"
     CDS             complement(31086..32174)
                     /gene="ompF"
                     /locus_tag="ACHOSM_RS13025"
                     /old_locus_tag="pgaptmp_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HGU4559100.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7896 a         7878 c         8723 g         7916 t
ORIGIN      
        1 aaaaaacgcc ccgtaatata actcagacta atcattaagc ctacattgcg taggctattt
       61 gattttattt tgcccagaat gctgctttgg cgcgctgcag cttttcgtag gccttcaaca
      121 acgactgatg tgcagcaaac gcgtgcagat cgctatctac tggttgcaag ccataaaacg
      181 ccgcttcgcc gctcattgcc gcactggcgg cttccacggc atctgcgccg tacatgcgaa
      241 caaacgcatt cagatattgc agcggctggc gatcttcttc ctgcgccagt aataacaacg
      301 tttgcaggca gcgataatag ttggcgcgtt ccgggctgaa taccgatgag ttaaactcca
      361 tcgtccattc ggtccagacc agagcctgtt ccagatcgcc accagccagc gccagcatgg
      421 cttttaattc accgatacgc agggtgtacc aaccgttatc cgacccggtc gccagaccca
      481 gcagctcacg cacgcgggta aagtcatcaa aaccttcttc atccagttgc tcgatgaggt
      541 tcaggtaatc ttctttttcc cactcgctgc ctggtagcga aagaatcgtt tcacgtaaat
      601 ggctgcccat actgttattc gcgagccaca gatcttcagc cggataaata tcggacatgc
      661 caggcacgat aatacggcaa gcataaacgc ccagatgctc gtaatcggca atataaactt
      721 ctttatcttc tttgttgaag atagccatca gcgtagcaaa ctcttcttcc gtggtgccgg
      781 agaaattcca gtccacaaac ggataatcgg catcctgctt gaacaggtcc caggagatta
      841 aaccgctgga atcgataaag tgcgtttcga ggttggtatg ttcagcgact tcttcatcat
      901 cgaaggttgg cggagtaaac acatccaaat ctttcaggcc acgaccttgc agcagctcgg
      961 tcacggtacg ttccagtgct acgccaaaat caggatgcgc accgaaagag gcaaagcagg
     1021 taccgttagc aggattgaac agtaccacgc aaatcaccgg atactggccg ccaagcgaac
     1081 cgtcataagc gaagattggg aaaccctccg cttccagcgt ttcgatggct tcaactactg
     1141 ctgggtaacg cgccagcacg tctgccggga tctctggcag gctgatgctt tcagcaataa
     1201 tgcggttttt cacgtagcgt tcgaaaactt cagacaatcc ctgaacgcgt gcttcgttgc
     1261 gggtattacc tgcggacata ccgttggaga cgtacaggtt accaatgata ttcatcggaa
     1321 tataaacggt ctgattgtcg gactgacgcg taaacggcag gccgcaaata ccacgatctt
     1381 cgttaccgga ttgtaggtca atcagcatgc tgccagtcag ttcattctcc ggatcataaa
     1441 acgcgcgcag acggtcatcg agtagtcctt ctggcacatc gtcattttcg gtcagtggga
     1501 accatttttc gttgggataa tgcacgaacg gaccgttggc gatggtttcg cccagccaga
     1561 aatcagcaaa aaagtagttg gttgagagac gctcgaaata ttcaccaagt gcagaagcca
     1621 gcgccgcttt tttggttgcg cctttaccgt tggtaaaaca cagtgcgcac tctttgtcgc
     1681 gaatgtgtac agaccagacg ttaggcacag gattcagcca ggaggcctct tcaatctgaa
     1741 agccgaggtc tgaaagtttt tgctggaagc gagcgatgga atcttccaga gcggcatctt
     1801 tgccggggat aaatgtttgc gtcatgaaaa tcactttagt cgtacggaaa gcgcgcaata
     1861 atacgggttt tatctcaaag gcgctatcac cgccgccatg ccgatgaact gttgactatg
     1921 cttttagcgg ataaaccacg ataagagcat aaaaatgaag gcgttcgatc tccaccgtat
     1981 ggcatttgat aaagtgcctt ttgatttcct tggcgaagtt gcactacgta gtctttatac
     2041 ctttgtactg gtttttttgt tcctcaaaat gaccggaaga cgcggtgtgc gacagatgtc
     2101 gctgtttgaa gtgttaatca ttctcacgct gggttcagcg gcgggagatg tggcgtttta
     2161 tgatgatgtg ccgatggtgc cggtacttat cgtctttatc actctggcgt tgttataccg
     2221 cctggtaatg tggttgatgg cgcacagtga aaaactggaa gatctactgg aaggtaagcc
     2281 ggttgttatt attgaagatg gcgagctggc ctggtcgaaa ctcaataact ccaacatgac
     2341 ggaatttgag ttctttatgg agctacgatt gagtggcgtg gagcagctgg ggcaggtacg
     2401 tctggcgatt ctcgaaacca acgggcaaat cagtgtctat ttctttgaag atgacaaggt
     2461 gaaaccgggt ttacttattt tacccagtga ttgtacgcag cgttacaaag tggtgccgga
     2521 gtcggcggac tatgcctgta ttcgttgtag tgaaatcatt catatgaaag cgggggaaaa
     2581 acaattatgt ccgcgctgtg caaatccaga atggacgaag gcaagtcggg caaaacgggt
     2641 gacctgacag taaaaacatc ggctttttgc taataatccg agagattctt ttgtgtgatg
     2701 caagccacat ttttgccctc aacggtttta ctcattgcga tgtgtgtcac tgaatgataa
     2761 aaccgatagc cacaggaata atgtattacc tgtggtcgca atcgattgac cgcgggttaa
     2821 tagcaacgca acgtggtgag gggaaatggc tcaaatcttc aattttagtt ctggtccggc
     2881 aatgctaccg gcagaggtgc ttaaacaggc tcaacaggaa ctgcgcgact ggaacggtct
     2941 tggtacgtcg gtgatggaag tgagtcaccg tggcaaagag ttcattcagg ttgcagagga
     3001 agccgagaag gattttcgcg atcttcttaa tgtcccctcc aactacaagg tattattctg
     3061 ccatggcggt ggtcgcggtc agtttgctgc ggtaccgctg aatattctcg gtgataaaac
     3121 caccgcagat tatgttgatg ccggttactg ggcggcaagt gccattaaag aagcgaaaaa
     3181 atactgcacg cctaatgtct ttgacgccaa agtgactgtt gatggtctgc gcgcgattaa
     3241 gccaatgcgt gaatggcaac tctctgataa tgctgcttat atgcattact gcccgaatga
     3301 aaccatcgac ggtatcgcca tcgacgaaac gccagacttc ggcaaagatg tggtggtcgc
     3361 cgccgacttc tcttcaacca ttctttcccg tccgattgac gtcagccgtt atggcgtgat
     3421 ttacgctggc gcgcagaaaa atatcggccc ggctggcctg acaatcgtca tcgttcgtga
     3481 agatttgctg ggcaaagcga atatcgcgtg tccgtcgatt ctggattatt ccatcctcaa
     3541 cgataacggc tccatgttta acacgccgcc gacatttgcc tggtatctat ctggtctggt
     3601 ctttaaatgg ctgaaagcga acggcggtgt agctgaaatg gataaaatca atcagcaaaa
     3661 agcagaactg ctatatgggg tgattgataa cagcgatttc taccgcaatg acgtggcgaa
     3721 agctaaccgt tcgcggatga acgtgccgtt ccagttggcg gacagtgcgc ttgacaaatt
     3781 gttccttgaa gagtcttttg ctgctggcct tcatgcactg aaaggtcacc gtgtggtcgg
     3841 cggaatgcgc gcttctattt ataacgccat gccgctggaa ggcgttaaag cgctgacaga
     3901 cttcatggtt gagttcgaac gccgtcacgg ttaatgccga aattttgctt aatccccaca
     3961 gccagcctgt ggggttttta tttctgttgt agagagttga gttcatggaa tccctgacgt
     4021 tacaacccat cgctcgtgtc gatggcacta ttaatctgcc cggttccaag agcgtttcta
     4081 accgcgcttt attgctggcg gcattagcac acggcaaaac agtattaacc aatctgctgg
     4141 atagcgatga cgtgcgccat atgctgaatg cattaacagc gttaggggta agctatacgc
     4201 tttcagccga tcgtacgcgt tgcgaaatta tcggtaacgg cggtccatta cacgcagaag
     4261 gtgccctgga gttgttcctc ggtaacgccg gaacggcaat gcgtccgctg gcggcagctc
     4321 tttgtctggg tagcaatgat attgtgctga ccggtgagcc gcgtatgaaa gaacgcccga
     4381 ttggtcatct ggtggatgcg ctgcgcctgg gcggggcgaa gatcacttac ctggaacaag
     4441 aaaattatcc gccgttgcgt ttacagggcg gctttactgg cggcaacgtt gacgttgatg
     4501 gctccgtttc cagccaattc ctcaccgcac tgttaatgac tgcgcctctt gcgccggaag
     4561 atacggtgat tcgtattaaa ggcgatctgg tttctaaacc ttatatcgac atcacactca
     4621 atctgatgaa gacgtttggt gttgaaattg aaaatcagca ctatcaacaa tttgtcgtaa
     4681 aaggcgggca gtcttatcag tctccgggta cttatttggt cgaaggcgat gcatcttcgg
     4741 cttcttactt tctggcagca gcagcaatca aaggcggcac tgtaaaagtg accggtattg
     4801 gacgtaacag tatgcagggt gatattcgct ttgctgatgt gctggaaaaa atgggcgcga
     4861 ccatttgctg gggcgatgat tatatttcct gcacgcgtgg tgaactgaac gctattgata
     4921 tggatatgaa ccatattccc gatgcggcga tgaccattgc cacggcggcg ttatttgcaa
     4981 aaggcaccac cacgctgcgc aatatctata actggcgtgt taaagaaacc gatcgcctgt
     5041 ttgcgatggc aacagaactg cgtaaagtcg gtgcggaagt agaagagggg cacgattaca
     5101 ttcgtatcac tccaccggaa aaactgaact ttgccgagat cgcgacatac aatgatcacc
     5161 ggatggcgat gtgtttctcg ctggtggcgt tgtcagatac accagtgacg attcttgatc
     5221 ccaaatgtac ggccaaaaca tttccggatt atttcgagca gttggcgcgg attagccagg
     5281 cagcctgaat gaacaacggg caataaatag ccaaatcttt ctttatcaaa acgtcggcac
     5341 attgtcggcg ttttttttcg gaccttgtga gtcattttga ttaatggtag cgtcgcttgt
     5401 caatgtaagt tgttgataca taatatttat atatgattaa tcaacggatg attcacatga
     5461 agaatactaa attactgctg gcgattgcga cctctgcagc attactgaca gggtgtcaaa
     5521 atacccacgg tattgatacc aatatggcta tcagctccgg tttaaatgcc tataaagcag
     5581 caacattaag cgatgccgat gcaaaagcga ttgccaatca gggctgtgcc gaaatggaca
     5641 gcggcaatca agtcgtaagt aaatccagca agtacggtaa acgtctagca aaaatcgcca
     5701 aagcattggg taacaatatt aatggcacgc cggtcaacta taaggtttat atgaccagcg
     5761 acgtcaacgc atgggcgatg gcgaacggct gtgtccgtgt ctacagtggc ctgatggaca
     5821 tgatgaatga caacgaaatt gaaggtgttc tgggccatga actgggccac gtcgcgttgg
     5881 gtcactcgct ggctgaaatg aaagcttctt atgcgatcgt tgccgcacgc gatgccattt
     5941 cagctaccag cggtgtggct tcccagcttt cccgctcaca attgggtgat atcgcagaag
     6001 gcgctattaa tgctaaatac tcccgtgata aagagtccga agcagatgat ttctcctttg
     6061 atctgttgaa gaaacgtggc atcagcaccc aggggctggt tggcagcttt gaaaaactgg
     6121 ctagtctgga tggcggtcgc acccagtcca tgtttgactc tcacccacca tcaacagagc
     6181 gtgcgcaaca catccgtgat cgtatcgcct ctggtaagta aatcattgtc atctttcggg
     6241 ctggtcttct gccagcccgc tataattgcg caataaatcc ccatctgaat acagacaaaa
     6301 ctggtttttg cacacaacgt taacgatttg tggcgtcggc gcgtataatg cgcgcggtta
     6361 tgttaacggt acgcctgttt taaggagata aagatgacgg caattgcccc ggttattacc
     6421 attgatggcc caagcggtgc agggaaaggc accttgtgta aggctatggc ggaagcattg
     6481 caatggcatc tgctggattc gggtgcaatt tatcgcgtac tggcattggc ggcattacat
     6541 caccatgttg atgttgcgtc agaagatgcg ctggtaccgc tggcatccca tctggatgta
     6601 cgttttgtgt caaccaatgg caatctggaa gtgatcctcg aaggggaaga tgtcagcggc
     6661 gaaattcgta ctcaggaagt ggcgaatgca gcttcacaag tcgcggcatt cccacgcgtt
     6721 cgtgaagcat tattgcgtcg ccaacgcgcg tttcgcgaat taccaggcct gattgccgat
     6781 ggccgcgaca tgggaacggt ggtattccct gatgcgccag tgaaaatttt ccttgacgcc
     6841 tcctcggaag aacgtgcgca tcgccgcatg ctacagttgc aggagaaggg ctttagtgtt
     6901 aactttgagc gccttttggc cgagatcaaa gaacgcgacg accgcgatcg taaccgagcg
     6961 gtagcgccac tggttccggc agccgatgct ttagtgttgg attccaccac cttaagcatt
     7021 gagcaagtga ttgaaaaagc gctacaatac gcgcgccaga aattggctct cgcataagcg
     7081 accgaatttg cagtaccccc gttgcaatgg aatgacagcg ggtatgttaa acaaccccat
     7141 ccggcatgga gccaggtgga cgttaaatat aaacctgaag attaaacatg actgaatctt
     7201 ttgctcaact ctttgaagag tccttaaaag aaatcgaaac ccgcccgggt tctatcgttc
     7261 gtggcgttgt tgttgctatc gacaaagacg tagtactggt tgacgctggt ctgaaatctg
     7321 agtccgccat cccggctgag cagttcaaaa acgcccaggg cgagctggaa atccaggtag
     7381 gtgacgaagt tgacgttgct ctggacgcag tagaagacgg cttcggtgaa actctgctgt
     7441 cccgtgagaa agctaaacgt cacgaagcct ggatcacgct ggaaaaagct tacgaagatg
     7501 ctgaaactgt taccggtgtt atcaacggca aagttaaggg cggcttcact gttgagctga
     7561 acggtattcg tgcgttcctg ccaggttctc tggtagacgt tcgtccggtg cgtgacactc
     7621 tgcacctgga aggcaaagag cttgaattta aagtaatcaa gctggatcag aagcgcaaca
     7681 acgttgttgt ttctcgtcgt gccgttatcg aatccgaaaa cagcgcagag cgcgatcagc
     7741 tgctggaaaa cctgcaggaa ggcatggaag ttaaaggtat cgttaagaac ctcactgact
     7801 acggtgcatt cgttgatctg ggcggcgttg acggcctgct gcacatcact gacatggcat
     7861 ggaaacgcgt taagcatccg agcgaaatcg tcaacgtggg cgacgaaatc actgttaaag
     7921 tgctgaagtt cgaccgcgaa cgtacccgtg tatccctggg cctgaaacag ttgggcgaag
     7981 atccgtgggt agctatcgct aaacgttatc cggaaggtac caaactgact ggtcgcgtga
     8041 ccaacctgac cgactacggc tgcttcgttg aaatcgaaga aggcgttgaa ggcctggtac
     8101 acgtttccga aatggattgg accaacaaaa acatccaccc gtccaaagtt gttaacgttg
     8161 gcgatgtagt ggaagttatg gttctggata tcgacgaaga acgtcgtcgt atctccctgg
     8221 gtctgaaaca gtgcaaagct aacccgtggc agcagttcgc ggaaacccac aacaagggcg
     8281 accgtgttga aggtaaaatc aagtctatca ctgacttcgg tatcttcatc ggcctggacg
     8341 gcggcatcga cggcctggtt cacctgtctg acatctcctg gaacgttgca ggcgaagaag
     8401 cagttcgtga atacaaaaaa ggcgacgaaa tcgctgcagt tgttctgcag gttgacgcag
     8461 aacgtgaacg tatctccctg ggcgttaaac agctcgcaga agatccgttc aacaactggg
     8521 ttgctctgaa caagaaaggc gctatcgtaa ccggtaaagt aactgcagtt gacgctaaag
     8581 gcgcaaccgt agaactggct gacggcgttg aaggttacct gcgtgcttct gaagcatccc
     8641 gtgaccgcgt tgaagacgcc accctggttc tgagcgttgg cgacgaagtt gaagctaaat
     8701 tcaccggcgt tgatcgtaaa aaccgcgcaa tcagcctgtc tgttcgtgcg aaagacgaag
     8761 ctgacgagaa agatgcaatc gcaactgtta acaaacagga agatgcaaac ttctccaaca
     8821 acgcaatggc tgaagctttc aaagcagcta aaggcgagta attctctgac tcttcgggat
     8881 ttttattccg aagtttgttg agtttacttg acagattgca ggtttcgtcc tgtaatcaag
     8941 cactaagggc ggctacggcc gcccttaatc aatgcagcaa cagcagccgc ttaatttgcc
     9001 tttaaggaac cggaggaatc atgaccaagt cagaattgat agaaagactt gccacccagc
     9061 aatcgcacat tcccgccaag acggttgaag atgcagtaaa agagatgctg gagcatatgg
     9121 cctcgactct tgcgcagggc gagcgtattg aaatccgcgg tttcggcagt ttctctttgc
     9181 actaccgcgc accacgtacc ggacgtaatc cgaagactgg cgataaagta gaactggaag
     9241 gaaaatacgt tcctcacttt aaacctggta aagaactgcg cgatcgcgcc aatatttacg
     9301 gttaagtttt tactcaaact tgaacgcgag aaaagcacct gtcgggtgct tttttcaatt
     9361 ctctaatcag gaactggaag ctgcctcgca gagttttgag cagttttcac cctttcgtta
     9421 aattcttctg aatgtgcctc ggggaacgca aaattcccac cagacaaccg ctcaacaaag
     9481 ttgcacactt tccataaaca gggaggggtg catgaaaata acgacagtcg gcgtatgcat
     9541 aatttgcgga atttttccgt tgctgatttt gccccaattg cctgggacat taacccttgc
     9601 gtttctgact ctcttcgcct gcgtactggc atttatccct gttaaaaccg tccgttatat
     9661 cgcgctgacg ttgctgtttt tcgtttgggg catattatca gcaaagcaaa ttttgtgggc
     9721 aggagaaacc ttaactggcg cgacgcagga tgcaattgtt gagatcactg caactgacgg
     9781 catgaccact cattacggtc aaattactta tctacaaggt cgacgtatat tccctgcgcc
     9841 aggccttgtg ctgtatggcg aatatcttcc gcaagcggtt tgtgctggac aacaatggtc
     9901 aatgaaactc aaagttcgtg cggttcatgg ccaacttaat gatggcggct ttgatagcca
     9961 gcgttatgcc attgcccagc atcaaccgct caccggccgc tttctgcagg caagtgttat
    10021 tgaaccgaat tgtagcctgc gtgcacagta tctggcgtca ctacaaacaa cgctgcaacc
    10081 ctatccgtgg aatgcggtta ttcttggttt aggtatgggg gaacggttat ccgtccccaa
    10141 agaaatcaaa aatatcatgc gcgatactgg aacggcgcat ttaatggcga tatcgggatt
    10201 gcacatcgct tttgcggcgt tgctggctgc cggactcatt cgcagtggac aaatttttct
    10261 gcctgggcgc tggatccact ggcaaatacc attaattggt gggatctgct gtgctgcttt
    10321 ttatgcctgg ttgacgggaa tgcaacctcc tgcattgcgt accgtggtgg cgcttgctac
    10381 gtggggaatg cttaagttaa gtgggcgaca gtggagtggc tgggatgtat ggatatgttg
    10441 tctggcggca attttgctga tggatcctgt tgccattctc tcgcaaagtt tatggctctc
    10501 tgccgctgcg gtcgcggcac tgattttttg gtatcagtgg tttccctgtc ctgagtggca
    10561 actgccgccg gtattgcgtg cagttgtttc cctcatccat ctgcaactgg gtatcacact
    10621 tctgcttatg cccgtgcaaa tcgtcatatt tcatggcatt agtctgacct cgtttattgc
    10681 aaatctatta gcaattccct tggtgacatt tatcacggtt ccgttgatcc tcgccgcgat
    10741 ggttgtgcat ttaagcgggc cgttaatcct ggagcaaggg ttatggtttc ttgccgaccg
    10801 gtctttggct ttacttttct gggggttaaa gagtttgcca gaagggtgga tcaacattgc
    10861 tgaacgttgg caatggctat cattttcccc atggttctta ctggtggtat ggcgattaaa
    10921 cgcctggcga acgttgccag caatgtgtgt ggctgtaggc ttgctgatgt gctggccgct
    10981 gtggcaaaaa cctcgacctg acgagtggca ggtgtacatg cttgatgtcg ggcaagggct
    11041 ggcaatggtg atagccagaa acggcaaagc gattctctat gacacaggac tggcctggcc
    11101 cgaaggggat agtgggcaac aactgattat cccctggctc cactggcata atcttgaacc
    11161 ggaaggcgtt attctgagtc atgaacatct ggatcaccgg ggagggctgg actcaatatt
    11221 gcacacatgg ccgatgttat ggatcagaag tccgttaaac tgggaacatc atcagccctg
    11281 tgtgcgtggc gaagcgtggc aatggcaagg attgcgtttc agcgtgcact ggcctttaca
    11341 agctagcaac gataaaggaa ataaccattc ctgtgtggtt aaggttgatg acgggacgaa
    11401 tagcattctt ctaaccggtg atattgaagt ccccgctgaa caaaagatgc taagccgtta
    11461 ctggcagcaa gtgcagacaa cattgcttca ggtacctcac catggcagta atacctcatc
    11521 atcgttgcca ttaattcagc gagtgaatgg aaaagtggca ctcgcatcgg catcgcgcta
    11581 taacgcatgg cgattgccct ctaataaagt taagcatcgc tatcaacagc aaggatatca
    11641 atggcttgat actccacatc agggtcaagt gacggtcaat ttttcagcgc aaggctggcg
    11701 gattagcagc ctcagagagc aaattttacc tcgttggtat catcagtggt ttggcgtgcc
    11761 agtggataac gggtagaata tgcggctatt tcaacaaatg ctggtttttt gaatgcataa
    11821 cgacaaagat ctctctacgt ggcagacgtt ccgccgactg tggccaacca ttgcgccttt
    11881 taaagcgggt ctgatcgtgg cgggcgtagc gttaatcctc aacgcagcca gcgatacctt
    11941 catgttatcg ctccttaagc cacttcttga tgatggcttt ggtaaaacag atcgctccgt
    12001 gctggtgtgg atgccgctgg tggtgatcgg gctgatgatt ttacgtggta tcaccagcta
    12061 tgtctccagc tactgtatct cctgggtatc aggaaaggtg gtaatgacca tgcgtcgccg
    12121 cctgtttggt cacatgatgg gaatgccggt ttcatttttt gacaaacagt caacggggac
    12181 gctgttgtca cgtatcacct acgattccga acaggttgct tcttcttctt ccggcgcact
    12241 gataacggtt gtgcgtgaag gtgcgtcaat catcggcctg ttcatcatga tgttctatta
    12301 cagttggcaa ctgtcgatca ttttgattgt gctggcaccg attgtttcga ttgcgattcg
    12361 cgtagtatcg aagcgttttc gcaacatcag taaaaacatg cagaacacca tggggcaggt
    12421 gaccaccagc gccgaacaaa tgctgaaggg ccacaaagaa gtattgattt tcggtggtca
    12481 ggaagtggaa acgaaacgct ttgataaagt cagcaaccga atgcgtcttc aggggatgaa
    12541 aatggtttca gcctcttcca tctctgatcc gatcattcag ctgatcgcct ctttggcgct
    12601 ggcgtttgtt ctgtatgcgg cgagcttccc aagtgtcatg gatagcctga ctgccggtac
    12661 gattaccgtt gttttctctt caatgattgc actgatgcgt ccgctgaaat cactgaccaa
    12721 cgttaacgcc cagttccagc gcggtatggc ggcttgtcag acgctgttta ccattctgga
    12781 cagtgagcag gagaaagacg aaggtaagcg cgtgatcgag cgtgcgactg gcgacgtgga
    12841 attccgcaat gtcaccttta cttatccggg acgtgacgta cctgcattgc gtaacatcaa
    12901 cctgaaaatt ccggcaggga agacggttgc tctggttgga cgctctggtt caggtaaatc
    12961 aaccatcgcc agcctgatca ctcgttttta cgatattgat gaaggcgaaa tcctgatgga
    13021 tggtcacgat ctgcgcgagt ataccctggc gtcgttacgt aaccaggttg ctctggtgtc
    13081 gcagaatgtc catctgttta acgatacggt tgctaacaac attgcttacg cacggactga
    13141 acagtacagc cgtgagcaaa ttgaagaagc ggcgcgtatg gcctacgcca tggacttcat
    13201 caataagatg gataacggtc tcgatacagt gattggtgaa aacggcgtgc tgctctctgg
    13261 cggtcagcgt cagcgtattg ctatcgctcg agccttgctg cgtgatagcc cgattctgat
    13321 tctggatgaa gctacctcgg ctctggatac cgaatccgaa cgtgcgattc aggcggcact
    13381 ggatgagttg cagaaaaacc gtacctctct ggtgattgcc caccgcttgt ctaccattga
    13441 aaaggcagac gaaatcgtgg tcgtcgagga tggtgtcatt gtggaacgcg gtacgcataa
    13501 cgatttgctt gagcaccgcg gcgtttacgc gcaacttcac aaaatgcagt ttggccaatg
    13561 atcgaaaaaa tctggtctgg tgaatcccct ttgtggcggc tattgctgcc actctcttgg
    13621 ttgtatggcc tggtgagtgg cgcgatccgt ctttgctata aactaaaact gaagcgcgcc
    13681 tggcgtgccc ccgtaccggt tgtcgtggtt ggtaatctca ccgcaggcgg caacggaaaa
    13741 accccggtcg ttgtctggct ggtggaacag ttgcaacagc gcggtattcg cgtgggggtc
    13801 gtatcgcggg gatatggtgg taaggctgaa tcttatccgc tgttattgtc ggcagatacc
    13861 acaacagcac aggcgggtga tgaacctgtg ttgatttatc aacgcactga tgcgcctgtt
    13921 gcggtttctc ccgttcgttc tgatgcggta aaagccattc tggcgcaaca ccctgatgtg
    13981 cagatcatcg taaccgacga cggtttacag cattaccgtc tggcgcgtga tgtggaaatt
    14041 gtcgttattg atggtgtgcg tcgctttggc aatggctggt ggttgccggc ggggccaatg
    14101 cgtgagcgag cggggcgctt aaagtcggtt gatgcggtaa tcgtcaacgg cggtgtccct
    14161 cgcagcggtg aaatccccat gcatctgctg ccgggtcagg cggtgaattt acgtaccggt
    14221 acgcgttgtg acgttgctca gcttgaacat gtggtggcga tggcagggat tgggcatccg
    14281 ccgcgctttt ttgccacgct gaagatgtgc ggcgtacaac cggaaaaatg tgtaccgctg
    14341 gccgatcatc agtctttgaa ccatgcggat gtcagcgcgt tggtaagcac cgggcaaacg
    14401 ctggtaatga ctgaaaaaga tgcggtaaaa tgccgggcct ttgcagaaga aaattggtgg
    14461 tatttgcccg ttgacgcaca gctttcaggc gatgaaccag cgaaactgct tgcgcaacta
    14521 acctcgctgg cttctggcaa ctagttacgc cgcggcagcg ttcgattgat ggagtcatga
    14581 atgtcgctgc cgcacctctc ccttgctgat gcgcgtaatc ttcaccttgc cgcgcaaggc
    14641 ctgttaaaca aaccccgccg tcgagcgtcg ttggaggata ttccggcaac gatctcccgc
    14701 atgtccttgc tgcaaatcga taccatcaat attgttgccc gtagtccata tctggtgctt
    14761 ttcagtcgtc tgggaaatta tcctgcccag tggctggatg agtctctggc gcgtggcgaa
    14821 ttaatggaat actgggcgca tgaagcctgc tttatgccgc gtagcgactt tcgtcttatt
    14881 cgccaccgca tgctggcacc tgaaaaaatg ggctggaaat acaaagacgc ctggatgcag
    14941 gaacatgagg cggaaattgc acagttaatt cagcatattc atgataaggg gccggtacgt
    15001 tcagccgatt ttgagcatcc tcgtaaaggt gcaagcggct ggtgggaatg gaagccgcat
    15061 aaacggcatc tggaaggttt atttactgcc ggaaaggtga tggtgattga acggcgcaac
    15121 ttccagcgcg tttatgattt aacccaccgt gtcatgcctg actgggatga tgagcgcgat
    15181 ctcgtttcgc aaacagaagc agaaatcatc atgctggata acagtgcgcg tagcctggga
    15241 atattccgcg aacagtggct ggcagattac tatcggctga aacgtccggc actggcggcg
    15301 tggcgcgaag cgagggctga acagcagcaa atcattgctg tgcatgttga aaaattgggc
    15361 aatctttggc tgcatgctga tttgctgccg ctactcgagc gtgcgctggc cggaaagctc
    15421 actgcaacgc acagcgcggt actttcgcct tttgatcctg ttgtctggga tcgcaaacgc
    15481 gcagagcagc tttttgattt tagctaccgg ctggagtgct ataccccagc gccgaaacgc
    15541 cagtatggct attttgttct gccattatta catcgtgggc aattagttgg gcgaatggat
    15601 gccaaaatgc atcgccagac aggcatcctt gaagttatct ctctgtggtt acaggaaggc
    15661 attaaaccaa cgacaatgct gcaaaaaggg ttacgtcagg cgattactga tttcgcgagc
    15721 tggcagcagg caacgcgggt gacattagga cgctgccccc aaggtctctt tactgattgc
    15781 cgcactggct gggaaataga ccccgtcgca taaatgatta tgataagatt tatgcattca
    15841 taacccgatc catctggagg aactatggat catcgtttgc ttgaaatcat tgcctgcccg
    15901 gtttgcaacg gaaaactttg gtataaccag gaaaaacaag agcttatttg caaactggat
    15961 aacctggctt tcccgctccg cgatggcatt ccagtgttgc tggaaacgga agcccgcgtg
    16021 ctgactgctg atgagagtaa atcatgagtt ttgtggtcat tattcccgcg cgctacgcgt
    16081 ccacgcgtct gcccggtaaa ccattggttg atattaacgg caaacccatg attgttcatg
    16141 ttcttgaacg cgcgcgtgaa tcaggtgccg agcgcatcat cgtggcaacc gatcatgagg
    16201 atgttgcccg cgccgttgaa gccgctggcg gtgaagtatg tatgacgcgc gccgatcatc
    16261 agtcaggaac agaacgtctg gcggaagttg tcgaaaaatg cgcattcagc gacgacacgg
    16321 tgatcgttaa tgtgcagggt gatgaaccga tgatccctgc gacaatcatt cgtcaggttg
    16381 ctgataacct cgctcagcgt caggtgggta tggcgactct ggcggtgcca atccacaatg
    16441 cggaagaagc gtttaacccg aatgcggtga aagtggttct cgacgctgaa gggtatgcac
    16501 tgtacttctc acgcgccacc attccttggg atcgtgatcg ttttgcagaa ggccttgaaa
    16561 ccgttggcga taacttcctg cgtcatcttg gtatttatgg ctaccgtgca ggctttatcc
    16621 gtcgttacgt caactggcag gcaagtccgt tagaacacat cgaaatgtta gagcagcttc
    16681 gtgttctgtg gtacggcgaa aaaatccatg ttgctgttgc tcaggaagtt cctggcacag
    16741 gtgtggatac ccctgaagat cttgagcgcg ttcgcgctga aatgcgctaa tttcacttca
    16801 cgacacttca gccaattttg ggaggagtgt cgtaccgtta cgattttcct caatttttct
    16861 tttcaacaat tgatctcatt caggtgacat cttttatatt ggcgctcatt atgaaagcag
    16921 tagcttttat gagggtaatc tgaatggaac agctgcgtgc cgaattaagc catttactgg
    16981 gcgaaaaact cagtcgtatt gagtgcgtca atgaaaaagc ggatacggcg ttgtgggctt
    17041 tgtatgacag ccagggaaac ccaatgccgt taatggcaag aagctttagt acgcccggaa
    17101 aagcccgaca actggcatgg aaaaccacca tgctggcaag aagtgggact gtccgtatgc
    17161 cgactattta tggtgtgatg acgcatgaag aacaccccgg ccctgatgtc ctgctactgg
    17221 agcggatgcg tggtgtttcg gtggaggcac cagcccgaac accagaacgc tgggaacagc
    17281 tcaaagacca aatcgttgaa gccttactgg cctggcaccg tcaggacagt cgcggttgcg
    17341 tcggcgcggt cgacaatact caggaaaatt tctggccctc atggtaccgg caacatgttg
    17401 aagtgctatg gaccacgctc aatcagttca ataacaccgg tctgacgatg caggataagc
    17461 ggatcctgtt tcgcactcgc gaatgtctcc cggcattatt tgaaggcttt aacgacaatt
    17521 gtgtgttgat tcacggtaac ttctgtttac gcagcatgct gaaagattcg cgcagcgatc
    17581 agttactggc gatggtcggg ccgggactaa tgctttgggc accgcgagaa tacgaactgt
    17641 tccgactaat ggataattct ctggcggaag atttgctctg gagttacctg caacgcgcgc
    17701 cagtggcgga gtcgttcatc tggcggcgtt ggttgtatgt gttatgggat gaagttgcgc
    17761 aactggttaa taccggacga tttagtcggc gcaacttcga tctggcatca aaatcactct
    17821 tgccgtggct cgcctgacgg acctttcagc cactgccaga tgcggccaag cgtttcatag
    17881 ccaacacgat cgctatgcat cagccagacc ggagaaggga ttgcccgttc ccacggatta
    17941 agcggcgagt cgatggccaa ctgattcgcc ggggctggta gcggatttaa cccttcctgc
    18001 tgaaaaaaga tcatcgcccg cggcagatgt gatgcggagg tcaccagcag gaaaggggcg
    18061 tcaccaatcg cctgtttcac tgccgcagct tcttcttcgg tatcttttgg caaatccagg
    18121 gtgataattt gctcgcgcgg cacgcccagc gattgcgcaa ctctggcacc tacttccgct
    18181 gtactcaccg tattggtttt tgctacgcct cccgtgaaga tcagttttga tcccggattt
    18241 tcgcgccata agcgaatacc ttcattcagg cgtggcagac tgttattgat taaattagag
    18301 ctcggtgccc actgcgggtt ccaggtataa ccacctccga gcaccacgat atagtccact
    18361 ttttgcgaat tattccatgt cggataggtg ctttcgatgg gacgcagcag gcggtcggct
    18421 accggttgca aactcaataa caagagtgcc agccacccaa tgctaataaa aatcttcgcg
    18481 gttttttgaa aacggctaaa ccacagcagc gccaggccag cgccaataat taacaacatc
    18541 agcggaagtg gcagcaacat attgccaatc acttttttca gtgtaaaaag catcctttct
    18601 ggttcctttt ttaaccatat agcaagggat cgccagcgat attacaccag acgggttcat
    18661 tctcgccgtg gctgtgacaa aatagcgtct ttatcagaaa acgtcttagc caaccggtgg
    18721 agaaagcaat gcaggatcgc aattttgatg atattgcgga aaagttttcc cgtaacattt
    18781 acggcaccac caaagggcag cttcgacagg ctattctgtg gcaggatctc gatcgcgtgc
    18841 tggcggaaat gggtccgcaa aaactgcgtg tgctggatgc gggcggtgga gaagggcaga
    18901 ccgcaatcaa aatggccgag cgcgggcatc aggtcatttt atgcgatctt tctgcgcaga
    18961 tgatcgaccg cgcaaaacag gcggcagaag caaaaggtgt gagcgacaac atgcaattta
    19021 tacattgcgc cgctcaggat gttgcttcgc atttggaaac gcccgttgat ctgatattgt
    19081 tccatgcggt gctcgagtgg gtggccgatc cccgcagtgt attgcagacc ctgtggtcag
    19141 tgttgcgtcc aggcggcgtg ttgtcgttaa tgttctacaa tgcgcatggt ttgttgatgc
    19201 ataacatggt cgccgggaat tttgattacg tgcaggcggg aatgccgaaa aagaaaaaac
    19261 ggacgctttc gccagattat ccacgcgacc cggcgcaggt ttatctgtgg ctggaagaag
    19321 ctggttggca aattatgggt aagacaggcg ttcgcgtgtt tcatgattat ctgcgcgaga
    19381 agcaccagca gcgcgactgc tatgaagcat tacttgaatt agaaacgcgt tattgccgtc
    19441 aggaaccgta tattaccctg gggcgttata ttcatgtcac cgcgcgcaaa ccgcagagca
    19501 aggataaagt atgagtgaat tttcccagac agtccccgaa ctggttgcct gggccagaaa
    19561 aaatgacttc tccatctcgc tgccggtaga ccgactctct tttctgctgg cggttgccac
    19621 gctgaacggc gagcgtctgg atggtgagat gagtgaaggc gagctggtgg atgcattccg
    19681 ccatgtgagt gatgcgtttg agcaaaccag cgaaaccatc ggcgtgcgcg ccaataacgc
    19741 gatcaacgac atggtgcgtc aacgtctgct gaaccgcttt accagcgagc aggcggaagg
    19801 gaacgcaatt taccgtctga cgccgctcgg catcggcatt actgactact acatccgtca
    19861 gcgcgagttt tctacgctgc gtctttctat gcagttgtcg attgtggcgg gtgagctcaa
    19921 acgcgcagca gatgccgccg aagagggcgg tgatgaattt cactggcacc gtaatgtcta
    19981 tgcgccactg aaatattcgg tagcagaaat tttcgacagt atcgacctga cgcaacgtct
    20041 gatggacgaa cagcagcagc aggtgaagga cgatatcgcc cagttgctga acaaagactg
    20101 gcgggcggcg atttccagct gtgaattgtt gctttcggaa acttccggaa cgctgcgtga
    20161 attgcaggat acgctggaag cggcaggcga caaattgcag gctaatctgt tgcgcattca
    20221 ggatgcgacg atgacccatg acgatctgca tttcgtcgat cgtctggtgt tcgatctgca
    20281 gagcaaactc gatcgtatta tcagttgggg ccagcaatcc atcgacttgt ggattggcta
    20341 cgaccgccac gtacacaaat ttattcgtac cgcgatcgat atggataaaa accgcgtctt
    20401 tgctcagcgg ttacgtcagt cggtacaaac ctattttgat gagccgtggg cgctaactta
    20461 tgccaatgcc gatcgtctgc tggatatgcg tgacgaagag atggcactgc gcgatgaaga
    20521 agtgactggg gaacttcctg aggatctgga atacgaagag tttaacgaga tccgcgaaca
    20581 gctggcggcg atcatcgaag aacaacttgc cgtgtacaaa accagacaag tgccgctgga
    20641 tcttggtctg gtggtacgcg aatatctgtc acagtatccg cgtgcacgtc actttgacgt
    20701 tgcgcgcatt gttattgatc aggcggtacg tcttggcgta gcgcaagcag atttcaccgg
    20761 actgccagcg aaatggcagc cgattaatga ttacggagcc aaggtacagg cgcatgtcat
    20821 cgacaaatat tgaacaagtg atgccggtta agctggcgca ggcgctggcg aatccgttat
    20881 ttccggcgct ggacagcgcc ttacgttcag gccgccatat tggcctcgac gaactggata
    20941 atcatgcatt cctgatggat tttcaggaat atctggaaga gttttacgcg cgttataacg
    21001 ttgagcttat tcgcgcaccg gaagggttct tctatttacg cccacgttcc accactctta
    21061 tcccgcgttc cgtcttgtcg gaactggata tgatggtcgg gaaaatcctc tgttatctct
    21121 atctcagccc ggaacggctg gcgaatgagg ggattttcac ccagcaggaa ctgtacgacg
    21181 aactgctcac cctggccgat gaagcaaaac tgctgaaact ggtgaacaac cgttcaaccg
    21241 gttcagacgt tgaccgtcag aagttgcagg agaaagtacg ttcttcgctc aaccgtctgc
    21301 gtcgtttagg catggtgtgg tttatgggcc acgacagcag caagtttcgc attaccgaat
    21361 cggtgttccg cttcggagcc gatgtgcgtg ctggcgacga tccccgtgaa gcacagcgtc
    21421 gcctgattcg tgatggcgaa gcaatgccga ttgaaaatca tctgcaactc aacgatgaaa
    21481 ccgaagagaa tcagccagat agcggagagg aagaataatg attgaacgcg gtaaatttcg
    21541 ctcactgacg ctgattaact ggaacggctt ttttgcccga acttttgacc ttgacgagct
    21601 ggtcacgacg ctttctggcg gtaacggggc gggtaaatcc accaccatgg cggcgttcgt
    21661 tacggcgctg atccccgacc tgaccctgct gcatttccgt aacactacgg aagccggggc
    21721 caccagcggt tcgcgcgata aaggtctgca cggtaagctg aaagcgggtg tctgttattc
    21781 gatgctcgac accattaact cgcgccacca gcgcgtggtg gtcggtgtgc gtctgcaaca
    21841 ggttgccgga cgcgatcgta aagtggatat caaaccattt gccattcagg gactgccgat
    21901 gtcggtgcag ccgacacagc tggtgaccga aaccctgaac gaacgccagg cgcgcgtgct
    21961 gccgcttaac gagctgaaag acaagctcga ggcgatggaa ggcgtgcagt ttaaacagtt
    22021 caactccatt accgattacc actcgctgat gttcgatctg ggcatcatcg cgcgtcgtct
    22081 gcgctccgca tctgaccgta gcaaattcta tcgtctgatc gaagcttcgc tgtatggcgg
    22141 gatctccagt gccattaccc gttctctgcg cgactacctg ttgccggaaa acagcggcgt
    22201 gcgtaaagcg ttccaggaca tggaagcggc gctgcgtgaa aaccgcatga cgctggaagc
    22261 gattcgtgtc acccagtcgg accgtgacct gtttaagcat ctgatcagcg aagctaccaa
    22321 ctacgtggcg gcggactaca tgcgtcacgc caacgagcgc cgtgtccatc tcgacaaagc
    22381 cctggagttt cgtcgcgagt tacatacttc gcgtcagcaa ctggcggctg agcagtacaa
    22441 acacgtcgat atggcgcgtg agctggcaga gcacaacggt gccgaaggcg atctggaagc
    22501 ggattatcag gcggccagcg atcacctgaa cctggtgcaa accgcactgc gtcagcagga
    22561 gaagatcgaa cgctacgaag cggatctcga tgagctgcag atccgtctcg aagagcaaaa
    22621 cgaagtggtg gcagaagcca tcgaacgcca ggaagagaac gaggctcgcg cggaagctgc
    22681 cgaactggaa gtggacgagc tgaaaagcca gcttgctgac taccagcagg cgctggacgt
    22741 ccagcaaacg cgcgcgatcc agtataacca ggcgattgct gcgcttaatc gcgccaaaga
    22801 actgtgccat ctgccggact taaccgccga cagcgccgcc gaatggctgg aaaccttcca
    22861 ggcgaaagag ctggaagcga ctgaaaaaat gctctctctt gagcagaaaa tgagcatggc
    22921 gcaaaccgcg cacagccagt ttgagcaggc ttatcagctg gtggtggcaa tcaacggccc
    22981 gctggcgcgt aacgaggctt gggatgtcgc ccgcgaactg ctgcgcgaag gggtcgatca
    23041 gcgtcacttg gcagagcagg ttcaaccgtt gcggatgcgc ttaagtgaac tggaacagcg
    23101 tctgcgcgag cagcaagaag ccgaacgcct gctggcagac ttctgcaaac gtcagggcaa
    23161 gaattttgat attgatgaac tggaagctct gcatcaggag ctggaagcgc gcattgcctc
    23221 tctttccgat agcgtgtcta acgcccgtga agagcgcatg gcactgcgcc aggaacagga
    23281 acagctgcag tctcgcattc agagtttgat gcagcgtgcg ccggtttggc tggcagcgca
    23341 aaacagtctc aaccagttga gcgagcagtg cggtgaagag tttacctcca gccaggacgt
    23401 caccgaatat ctgcaacagt tgctggagcg tgagcgagag gcgattgttg aacgcgatga
    23461 agtgggcgcg cgcaaaaacg ctgtcgatga agagatcgaa cgtttaagcc agcctggcgg
    23521 ctctgaagat cagcgtctga acgcgctggc ggagagtttt ggcggcgtgc tgctgtcaga
    23581 aatttatgac gacgttagcc tggaagatgc gccgtacttc tcagcgctgt atggcccgtc
    23641 acgccacgcc atcgtggtgc cagatctgtc acaggtaact gaacacctgg aaggcttgac
    23701 cgattgcccg gaagatctct atttgatcga aggggatccg cagtcattcg atgacagcgt
    23761 gttcagcgtt gatgagctgg aaaaagcggt agtggtgaaa atcgccgatc gtcagtggcg
    23821 ttattcacgt ttcccggaag tgccgctgtt tggtcgtgct gcgcgtgaaa gccgtattga
    23881 aagcctccat gccgagcgtg aagtgctttc cgaacgcttc gccacgctct cctttgatgt
    23941 acagaaaacc cagcgtctgc atcaggcgtt cagccgcttt atcggcagtc atctggcggt
    24001 ggcgtttgag tctgacccgg aagcagaaat ccgtcaactg aacagccgtc gcgtcgaact
    24061 ggagcgggcg ttgagtaatc atgaaaatga taaccagcag cagcgtattc agtttgagca
    24121 ggcgaaagaa ggcgttacgg cgctgaaccg cattctgccg cgtctcaacc tgttggctga
    24181 tgacagcctg gcggatcgcg tcgatgaaat ccgcgaacgt ctggatgaag cccaggaagc
    24241 cgcgcgtttt gttcagcagt ttggcaatca actggcgaaa ctggaaccga tcgtttcggt
    24301 attgcagagc gacccggaac agttcgaaca gctaaaagaa gattacgcgt actctcagca
    24361 gatgcagcgc gatgcccgtc agcaggcgtt tgccctgacg gaagtggtgc agcgtcgtgc
    24421 gcactttagc tattctgact cggcagaaat gctgagcggt aatagcgatc tcaacgaaaa
    24481 gctgcgtgaa cgtctggaac aggcggaagc ggagcgtact cgcgctcgtg aagcgttgcg
    24541 tggtcacgca gcgcagttga gtcagtacaa ccaggtgctg gcttcgctga aaagttctta
    24601 cgacaccaaa aaagagctac tcaacgatct gcaacgtgaa ttgcaggata tcggcgtgcg
    24661 cgccgatagc ggggcagaag agcgggcgcg aattcgccgt gacgagctgc atgcgcaact
    24721 gagtaataac cggtcccgcc gcaaccagct ggaaaaagca ctgactttct gcgaagcgga
    24781 gatggacaat ctgacccgca aactgcgcaa gctggagcgg gattactttg agatgcgcga
    24841 gcaggtagtg accgccaaag cgggctggtg tgcagtgatg cgcatggtga aagataacgg
    24901 cgttgaacgg cgcttacacc gtcgcgagct ggcttatctc tccgctgatg atttgcgctc
    24961 tatgtcggat aaggcattgg gtgcgctgcg tctggcggtg gcggataacg aacatctgcg
    25021 cgacgtgctg cgcatgtcgg aagatccgaa acgtccggag cgtaaaattc agttcttcgt
    25081 ggcggtttat cagcatctgc gtgaacgtat tcgtcaggat attattcgta ccgatgatcc
    25141 ggtggaagct atcgaacaga tggagatcga acttagccgt ctgaccgaag aattaacctc
    25201 ccgtgaacag aaactggcga tcagttcccg cagcgtggcg aacatcattc gcaaaaccat
    25261 tcagcgcgag cagaaccgta tccgtatgct caaccagggg ttgcagaacg tatcgtttgg
    25321 tcaggtgaac agcgtgcgcc tcaacgtgaa cgtgcgtgaa acgcacgcca tgctgctgga
    25381 tgtgctctct gaacagcacg agcagcatca ggatctgttt aacagcaacc gtctgacctt
    25441 ctcggaagcg ttggcgaaat tgtatcagcg tcttaatccg cagattgata tggggcagcg
    25501 cacgccgcag accattggcg aagaactgct ggattaccgc aactatctgg aaatggaagt
    25561 tgaggttaat cgtggttccg atggttggtt gcgcgcagag tctggtgcat tgtcgaccgg
    25621 tgaggcgatt ggtaccggta tgtcgattct ggtgatggtg gtacaaagct gggaagatga
    25681 atctcgccgc ctgcgcggta aagatatctc tccttgccgc ctgctgttcc tcgatgaagc
    25741 agcgcgactg gatgctcgtt ctatcgccac gctgtttgaa ttgtgtgaac gtttgcaaat
    25801 gcagctcatc atcgcagcgc cggaaaatat cagcccggag aaaggcacca cctataaact
    25861 ggtgcgtaaa gtcttccaga ataccgaaca cgttcacgtc gtcggcctgc gaggatttgc
    25921 gccgcaactc cctgaaacgc ttccaggaag tgacgaagcg ccttctcagg cgagttaaaa
    25981 ttaaggcggc agcaatgccg ccttttcttt ttccgaaaac tccgtttctg cactaaaaaa
    26041 gtggcacatt acggcgcgct tttctttaaa cttctttaca ttaggttatg taaaaacgta
    26101 tcggcgttta tatactgaag ataagcctga tgagtaacag gcttgctcgt catactttcg
    26161 tgagtattgg cgttgtacag gcaagtcgta aaataacagc ctggctattc agagtatgat
    26221 aaaaacaggg ggcaagggat gttgcttaat atgatgtgtg gtcgtcggct gtcggcaatc
    26281 agtttgtgcc tggccgtaac attcgctcca ctgttcaatg cgcaggccga tgagcctgaa
    26341 gtaatccctg gcgacagccc ggtggctgtc agtgaacagg gcgaggcact gccgcaggcg
    26401 caagccacgg caataatggc ggggatccag ccattgcctg aaggtgcggc agaaaaagcc
    26461 cgcacgcaaa tcgaatctca attacccgca ggttacaagc cggtttatct taaccagctt
    26521 caactgttgt atgccgcacg cgatatgcaa cccatgtggg aaaaccgtga tgctgttaaa
    26581 gccttccagc aacagctggc agagttggcg attgccggtt tccagccgca gtttaataaa
    26641 tgggtagagt tactgaccga tcctggtgtt aacgggatgg cacgcgacgt agtgctctct
    26701 gatgcgatga tgggctatct ccatttcatt gcaaatattc cggtcaaagg cactcgctgg
    26761 ctatatagca gtaaacctta tgcacttgca acgccgccgc tctcggtgat taaccaatgg
    26821 cagctggcgc tggataaagg tcaattgcct acgtttgttg caggactggc accgcagcat
    26881 ccgcaatatg cggcgatgca tgaatcgtta ctggccttac tcagtgacac caaaccgtgg
    26941 ccccaactga ccggaaaagc aacgttgcgc ccagggcagt ggagtaacga cgtaccggcg
    27001 ttgcgcgaaa tattgcaacg cacaggcatg ttggacgggg ggctgaaaat tactctacct
    27061 ggcgatgaca cgccaactga cgcggtagtc agcccatccg ctgttactgt tgaaacagca
    27121 gaaactaagc cgatggacaa gcaaacgacg tctcgtagta aacctgcgcc tgccgttcgc
    27181 gccgcctacg ataatgaact ggtggaagcc gttaaacgtt ttcaggcatg gcaaggattg
    27241 ggggcagatg gtgctattgg cccggcaaca cgtgactggt taaacgtaac gcccgcccag
    27301 cgtgctggcg tgttggctct caacatccag cgattgcgct tgctgccaac agagctttct
    27361 accgggatca tggttaacat tccggcctat tcgctggtct actatcagaa cggcaatcag
    27421 gtgctggatt cgcgagtcat tgtcggtcgc cccgatcgca aaacgccgat gatgagcagt
    27481 gcccttaaca acgtagtggt aaacccgccg tggaacgtac ctccaactct ggcacgcaaa
    27541 gatattctgc caaaagtgcg caacgatccg ggatatctcg aaagccatgg ctatacggtg
    27601 atgcgcggct ggaacagcag agaagcgatt gacccatggc aggttgactg gtctacaatc
    27661 acggcctcga atttaccgtt ccgcttccag caggctccag gcccacggaa ctcgctgggg
    27721 cgctataaat tcaatatgcc gagttcagag gccatttatt tgcatgacac gccgaaccac
    27781 aatctgttca agcgtgatac acgcgcattg agctcaggct gtgtacgagt gaataaagct
    27841 tccgatctgg cgaatatgct gttgcaggat gcaggctgga atgacaaacg tatttctgat
    27901 gcgctgaagc agggtgatac acgttacgtc aatattcggc agtcgattcc ggtgaatctc
    27961 tactacctga cggcctttgt tggtgcagat ggtcgtaccc agtatcgtac agatatttac
    28021 aattatgatc tgcctgcgcg atccagctcg caaatcgtat cgaaagcgga acaattaatc
    28081 aggtaaatga agtagttcgg ggaaatcagt tgtcgtaata attagcatga ttgggggcga
    28141 ttctctgcag cccccgtcac tgctggggtt gagtcatctt gacgtctgct ttacgggcgg
    28201 ttaaggtgcc tcttgtgcgc cagaagtgca tataaacgat aacattgacc tgtagacttg
    28261 attatcatgg acaaattcga cgctaatcgc cgcaaattgc tggcgcttgg tggcgttgca
    28321 ctcggtgccg ccatcctgcc gacccctgcg tttgcaacac tctctacccc acgcccgcgc
    28381 attttgatac tcaataatct tcataccgga gagtcaatca aagcggagtt tttcgatggc
    28441 agaggctata ttcaggaaga attggcaaaa cttaaccatt ttttccgcga ttaccgcgcg
    28501 aacaaaataa agtccatcga cccaggatta ttcgaccagt tgtatcgcct gcaagggttg
    28561 ttaggcacgc gcaaaccggt gcaactcatt tccggttatc gttctattga taccaacaat
    28621 gaactacgcg cccgcagccg tggagtagcg aagaaaagct atcacactaa aggccaggcg
    28681 atggatttcc atattgaagg tatcgcgtta agcaatattc gcaaagccgc gttatctatg
    28741 cgcgcaggtg gtgtaggata ttatcctcgt agtaactttg tgcatattga taccgggcca
    28801 gcacggcact ggtagtaatt gcttaacgaa acaggggcag tatgaactat cgtattattc
    28861 cggtcaccgc attctcccag aactgttcat taatctggtg tgaacaaacc cgtctggccg
    28921 cactggtcga tcctggcggc gatgcggaaa aaatcaaaca ggaagttgat gacagcggcc
    28981 tgacactgat gcagatcctg ctgacgcatg gtcatctgga ccacgttggc gcagcggcgg
    29041 agctggcgca acattacggc gtgccggttt ttggcccgga aaaagaagat gagttctggc
    29101 tgcaaggctt gcctgcgcaa agtcgtatgt ttggtctgga agagtgccag ccgctgacgc
    29161 cagatcgttg gctgaacgaa ggcgatacca tcagcatagg gaatgtgact ttacaggtgt
    29221 tacattgccc tgggcatacg ccgggtcatg tcgtgttttt tgatgatcgg gcaaagctgc
    29281 tgatttctgg cgatgttatt ttcaaaggcg gagtagggcg cagtgacttc ccgcgtggcg
    29341 atcataatca actgatttct tcaatcaaag ataaattgct gccactgggg gatgacgtga
    29401 catttattcc gggtcacgga ccattatcca cacttggtta tgaacgcctg cataatccct
    29461 tcctgcaaga cgaaatgccc gtctggtaag gcacataaaa aagcccgctt ttaatgcggg
    29521 cctggatttc tggcaaagtg cgctttgttt atgccggatg cggcacgagc gccttatccg
    29581 gcctacaaaa tcgtgcaaat tcaaaatatt gcaggggacg cgtaggcctg ataagcgtag
    29641 cgcatcaggc aatgttgcgt ttgtcatcag tctcagcccg cttttcagcg ggcttcattg
    29701 tttttaatgc ttacagcact gccacaatcg cttcgcacag cggagccatg ttatctggtg
    29761 tcatcccggc cacatttacg cgaccagaag caaccgcata tacgccaaac tcttcgcgca
    29821 gacgcagcac ttgttctttt gtcaggccac tgaaggagaa catgccgttc tgtttgatga
    29881 taaagctgaa gtcgcggttt gcgccttttt cctgcagcgt attgacgaac aactgacgca
    29941 tacgctgaat acgctggcgc atatcagtca gctcttgttc ccaaatcgca cgtaacgcat
    30001 cgttgctcag gatggtggca acaacagaag cgccgtgtgc tggtgggtta gagtagttag
    30061 cgcgaatcgc cgctttcatt tggctgaatg cgcgatcaac ggtttcactg tcggcagcaa
    30121 ccagagtaca agcgccaaca cgctcgttgt acaggccaaa gtttttagag taggaactgg
    30181 caacaatcag ctctttatgc atagccgcga aagcgcgcag tccttcagca tcttcttcca
    30241 gaccacgggc aaaaccctgg taagcgaagt caaacagcgg taaccagcct ttctcaacgg
    30301 agagttgtgc cagtgtctgc cattgttcca gcgtagggtc gataccggtt gggttatggc
    30361 agcagccatg gaacagcact acgtcgccag cctgagcttc attcaggctg ttaatcagtg
    30421 catcgaagtc aagagtgtga ttttccgcat cataataagc gtattcacga acctccagac
    30481 ctgcagagtt aaagacgctc ttatggttcg gccagcttgg gttgctcacc cacactcgct
    30541 taacgctggt attttttgcc aggaaatcgg cagctacgcg tagtgcgcca gtgccccccg
    30601 gagtctgtgc cgtgcgagca cgtttgtcat tgatcagggc gctaccttta ccaaacagca
    30661 gttcctgagt gcagcgacca aattcaggga tgccgtcaat gccgaggtaa tttttggtgg
    30721 tttcattttc gagcagatac tgttcagcct ttttcacgct ggtcagtacc ggggttttgc
    30781 ccgtctcatc tttatagaca ccaatcccga ggttaatttt gccgggacgt tcatcggcac
    30841 gaaacagatc ggccaggccc agaatcgggt cggcaggagc ggcggtaatg ttctcaaaca
    30901 tgacgaggtt ccattatggt tacagaaggg aagtccgcta tcagggtaac gggagattta
    30961 caaaattcca actattactg atgaaaacgc aggctgtttt tgcaagacgt gagattgctc
    31021 tggaaggtat aaaaaaaaca ggaccaaagt cctgtttttt cggcatttaa caaagaggtg
    31081 tgctattaga actggtaaac gatacccaca gcaacggtgt cgtctgaacc tacgcccagt
    31141 ttgttgtcag aatcgatctg gttgatgatg tagtcaacat aggtggacat gtttttgttg
    31201 aagtagtagg ttgcgcccac ttcaaagtag ttcaccagat caacatcacc gataccttct
    31261 acgtctttcg ctttagattt ggtgtaagcg atggacggac gcagaccgaa atcgaactgg
    31321 tattgcgcaa ctaacagaac gtcttgcgtt ttgttggcga agccgctggt gtttgtaaat
    31381 ttattagtga tcggcgtagc gttacgggtt tcaccgtagt tcgctgccag gtagatgttg
    31441 ttcgcgtcgt acttcagacc agtagcccac tgttcagctt ttttaccgtt gccaagaggt
    31501 tgagcttctt gcaggttggt acggtcagct gcaccataag caccaacgat accaaagcct
    31561 tcgtattcgt agctgataga accgccaaca ccgtcgccgt tagaacggcg tgcagtgtca
    31621 cgctcgtttt tacccaggta ctgaacagcg aagttcaggc catcaaccag accaaagaag
    31681 ttggagttac gataggtagc aacgccgcca acacgaccaa cgaagaagtc atcgctgtat
    31741 gcagtatcac caccaaattc tggcagcata tcggtgtaac ccagtgcatc ataaaccaca
    31801 ccgtagttac ggccgtaatc gaaagaacca acgtcagcgt atttaagacc cgcgaatgcc
    31861 agacgcgttt tgttaccagt ttgagcgtca gcgccttcag agttgttacc ctggaagtta
    31921 tattcccact gaccataacc ggtcagatcg gaattgattt gagtttcccc tttaaaacca
    31981 agacgggcat aggtcatgtc gccattgcca ccgtaactgt tttcaccgtt acccttggaa
    32041 aaataatgca gaccaacagc tttaccgtac agatctactt tgttgccatc tttgttatag
    32101 atttctgcag cgtttgcagt acctgctact aacagagcag ggacgatcac tgccagaata
    32161 ttgcgcttca tcattattta ttaccctcat ggtttttttt atgacacctg ccactgccgt
    32221 caataagttc tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt
    32281 gtctgcaggc atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctacaaagat
    32341 aaagatttag tttcaaaaag aaaaatatgt aaccaaaagt aaaaattaag gaactttgtg
    32401 aacaccgtca tat
//
LOCUS       DAZKHC020000049        31952 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZKHC020000049 DAZKHC020000000
VERSION     DAZKHC020000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.049"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            43..342
                     /locus_tag="ACHOSM_RS13030"
                     /old_locus_tag="pgaptmp_002606"
     CDS             43..342
                     /locus_tag="ACHOSM_RS13030"
                     /old_locus_tag="pgaptmp_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559101.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSYFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(389..1276)
                     /gene="rarD"
                     /locus_tag="ACHOSM_RS13035"
                     /old_locus_tag="pgaptmp_002607"
     CDS             complement(389..1276)
                     /gene="rarD"
                     /locus_tag="ACHOSM_RS13035"
                     /old_locus_tag="pgaptmp_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HGU4559102.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1328..1795)
                     /gene="yigI"
                     /locus_tag="ACHOSM_RS13040"
                     /old_locus_tag="pgaptmp_002608"
     CDS             complement(1328..1795)
                     /gene="yigI"
                     /locus_tag="ACHOSM_RS13040"
                     /old_locus_tag="pgaptmp_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HGU4559103.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1960..2829
                     /gene="pldA"
                     /locus_tag="ACHOSM_RS13045"
                     /old_locus_tag="pgaptmp_002609"
     CDS             1960..2829
                     /gene="pldA"
                     /locus_tag="ACHOSM_RS13045"
                     /old_locus_tag="pgaptmp_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HGU4559104.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2962..4791
                     /gene="recQ"
                     /locus_tag="ACHOSM_RS13050"
                     /old_locus_tag="pgaptmp_002610"
     CDS             2962..4791
                     /gene="recQ"
                     /locus_tag="ACHOSM_RS13050"
                     /old_locus_tag="pgaptmp_002610"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HGU4559105.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4855..5475
                     /gene="rhtC"
                     /locus_tag="ACHOSM_RS13055"
                     /old_locus_tag="pgaptmp_002611"
     CDS             4855..5475
                     /gene="rhtC"
                     /locus_tag="ACHOSM_RS13055"
                     /old_locus_tag="pgaptmp_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HGU4559106.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5537..6157)
                     /gene="rhtB"
                     /locus_tag="ACHOSM_RS13060"
                     /old_locus_tag="pgaptmp_002612"
     CDS             complement(5537..6157)
                     /gene="rhtB"
                     /locus_tag="ACHOSM_RS13060"
                     /old_locus_tag="pgaptmp_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HGU4559107.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALIPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6268..7290
                     /gene="pldB"
                     /locus_tag="ACHOSM_RS13065"
                     /old_locus_tag="pgaptmp_002613"
     CDS             6268..7290
                     /gene="pldB"
                     /locus_tag="ACHOSM_RS13065"
                     /old_locus_tag="pgaptmp_002613"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HGU4559108.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLEFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7298..8098
                     /gene="yigL"
                     /locus_tag="ACHOSM_RS13070"
                     /old_locus_tag="pgaptmp_002614"
     CDS             7298..8098
                     /gene="yigL"
                     /locus_tag="ACHOSM_RS13070"
                     /old_locus_tag="pgaptmp_002614"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HGU4559109.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8174..9073
                     /gene="bioP"
                     /locus_tag="ACHOSM_RS13075"
                     /old_locus_tag="pgaptmp_002615"
     CDS             8174..9073
                     /gene="bioP"
                     /locus_tag="ACHOSM_RS13075"
                     /old_locus_tag="pgaptmp_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HGU4559110.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVSLASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8961..9914)
                     /gene="metR"
                     /locus_tag="ACHOSM_RS13080"
                     /old_locus_tag="pgaptmp_002616"
     CDS             complement(8961..9914)
                     /gene="metR"
                     /locus_tag="ACHOSM_RS13080"
                     /old_locus_tag="pgaptmp_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HGU4559111.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10032..12293
                     /gene="metE"
                     /locus_tag="ACHOSM_RS13085"
                     /old_locus_tag="pgaptmp_002617"
     CDS             10032..12293
                     /gene="metE"
                     /locus_tag="ACHOSM_RS13085"
                     /old_locus_tag="pgaptmp_002617"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGU4559112.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLSTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12332..13147)
                     /gene="ysgA"
                     /locus_tag="ACHOSM_RS13090"
                     /old_locus_tag="pgaptmp_002618"
     CDS             complement(12332..13147)
                     /gene="ysgA"
                     /locus_tag="ACHOSM_RS13090"
                     /old_locus_tag="pgaptmp_002618"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HGU4559113.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLAGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13409..14170
                     /gene="udp"
                     /locus_tag="ACHOSM_RS13095"
                     /old_locus_tag="pgaptmp_002619"
     CDS             13409..14170
                     /gene="udp"
                     /locus_tag="ACHOSM_RS13095"
                     /old_locus_tag="pgaptmp_002619"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HGU4559114.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14311..15738
                     /gene="rmuC"
                     /locus_tag="ACHOSM_RS13100"
                     /old_locus_tag="pgaptmp_002620"
     CDS             14311..15738
                     /gene="rmuC"
                     /locus_tag="ACHOSM_RS13100"
                     /old_locus_tag="pgaptmp_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGU4559115.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKISSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15833..16588
                     /gene="ubiE"
                     /locus_tag="ACHOSM_RS13105"
                     /old_locus_tag="pgaptmp_002621"
     CDS             15833..16588
                     /gene="ubiE"
                     /locus_tag="ACHOSM_RS13105"
                     /old_locus_tag="pgaptmp_002621"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HGU4559116.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16602..17207
                     /gene="ubiJ"
                     /locus_tag="ACHOSM_RS13110"
                     /old_locus_tag="pgaptmp_002622"
     CDS             16602..17207
                     /gene="ubiJ"
                     /locus_tag="ACHOSM_RS13110"
                     /old_locus_tag="pgaptmp_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HGU4559117.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17204..18844
                     /gene="ubiB"
                     /locus_tag="ACHOSM_RS13115"
                     /old_locus_tag="pgaptmp_002623"
     CDS             17204..18844
                     /gene="ubiB"
                     /locus_tag="ACHOSM_RS13115"
                     /old_locus_tag="pgaptmp_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HGU4559118.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18923..19192
                     /gene="tatA"
                     /locus_tag="ACHOSM_RS13120"
                     /old_locus_tag="pgaptmp_002624"
     CDS             18923..19192
                     /gene="tatA"
                     /locus_tag="ACHOSM_RS13120"
                     /old_locus_tag="pgaptmp_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HGU4559119.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19196..19711
                     /gene="tatB"
                     /locus_tag="ACHOSM_RS13125"
                     /old_locus_tag="pgaptmp_002625"
     CDS             19196..19711
                     /gene="tatB"
                     /locus_tag="ACHOSM_RS13125"
                     /old_locus_tag="pgaptmp_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HGU4559120.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19714..20490
                     /gene="tatC"
                     /locus_tag="ACHOSM_RS13130"
                     /old_locus_tag="pgaptmp_002626"
     CDS             19714..20490
                     /gene="tatC"
                     /locus_tag="ACHOSM_RS13130"
                     /old_locus_tag="pgaptmp_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HGU4559121.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20532..21314
                     /gene="tatD"
                     /locus_tag="ACHOSM_RS13135"
                     /old_locus_tag="pgaptmp_002627"
     CDS             20532..21314
                     /gene="tatD"
                     /locus_tag="ACHOSM_RS13135"
                     /old_locus_tag="pgaptmp_002627"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HGU4559122.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21311..21799)
                     /gene="rfaH"
                     /locus_tag="ACHOSM_RS13140"
                     /old_locus_tag="pgaptmp_002628"
     CDS             complement(21311..21799)
                     /gene="rfaH"
                     /locus_tag="ACHOSM_RS13140"
                     /old_locus_tag="pgaptmp_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HGU4559123.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21966..23459
                     /gene="ubiD"
                     /locus_tag="ACHOSM_RS13145"
                     /old_locus_tag="pgaptmp_002629"
     CDS             21966..23459
                     /gene="ubiD"
                     /locus_tag="ACHOSM_RS13145"
                     /old_locus_tag="pgaptmp_002629"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HGU4559124.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23505..24206
                     /gene="fre"
                     /locus_tag="ACHOSM_RS13150"
                     /old_locus_tag="pgaptmp_002630"
     CDS             23505..24206
                     /gene="fre"
                     /locus_tag="ACHOSM_RS13150"
                     /old_locus_tag="pgaptmp_002630"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HGU4559125.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24398..25561)
                     /gene="fadA"
                     /locus_tag="ACHOSM_RS13155"
                     /old_locus_tag="pgaptmp_002631"
     CDS             complement(24398..25561)
                     /gene="fadA"
                     /locus_tag="ACHOSM_RS13155"
                     /old_locus_tag="pgaptmp_002631"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HGU4559126.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25571..27760)
                     /gene="fadB"
                     /locus_tag="ACHOSM_RS13160"
                     /old_locus_tag="pgaptmp_002632"
     CDS             complement(25571..27760)
                     /gene="fadB"
                     /locus_tag="ACHOSM_RS13160"
                     /old_locus_tag="pgaptmp_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HGU4559127.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKLKKEEDTAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27950..29281
                     /gene="pepQ"
                     /locus_tag="ACHOSM_RS13165"
                     /old_locus_tag="pgaptmp_002633"
     CDS             27950..29281
                     /gene="pepQ"
                     /locus_tag="ACHOSM_RS13165"
                     /old_locus_tag="pgaptmp_002633"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HGU4559128.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29281..29895
                     /gene="yigZ"
                     /locus_tag="ACHOSM_RS13170"
                     /old_locus_tag="pgaptmp_002634"
     CDS             29281..29895
                     /gene="yigZ"
                     /locus_tag="ACHOSM_RS13170"
                     /old_locus_tag="pgaptmp_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HGU4559129.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGVVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29934..31385
                     /gene="trkH"
                     /locus_tag="ACHOSM_RS13175"
                     /old_locus_tag="pgaptmp_002635"
     CDS             29934..31385
                     /gene="trkH"
                     /locus_tag="ACHOSM_RS13175"
                     /old_locus_tag="pgaptmp_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HGU4559130.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31397..31942
                     /gene="hemG"
                     /locus_tag="ACHOSM_RS13180"
                     /old_locus_tag="pgaptmp_002636"
     CDS             31397..31942
                     /gene="hemG"
                     /locus_tag="ACHOSM_RS13180"
                     /old_locus_tag="pgaptmp_002636"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HGU4559131.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7409 a         8111 c         8743 g         7689 t
ORIGIN      
        1 atttttattc tataaacaat atcttgggtg aatggattct atatgggaaa tggtaacttt
       61 tggcagttgt tcttgggtaa agaaccttac agcaaaaaat cttattttgt taaggcatgg
      121 ttattgtggt gggctattac catccttgtt ctatttattt taagcctgac tacgacatta
      181 aatttaagaa gtataggtat agggttaagt tttgggtttt gtcaaatcct tggtagatat
      241 acgatgtctc taggatggag taagtactgg ggagttttgg gggtatttcc tgtaacaaat
      301 attgttcttt ttcttatctt aatttttttc aaaaaaagat aaataagtca gtgggtaggc
      361 tctttaaacg gcctacccat gttaattctt aactttttct acgctgcgta taaatcgcat
      421 ccatcacaaa aattgccagc gccacccaaa taaaggcgaa agtcaccatc ttatcggcac
      481 ccggtttttc accataaaac gtcacagcca gcaggaacat cagcgtcggg ccaatgtact
      541 ggaaaaagcc taacgttgag agacgcaagc gcgtggcagc ggcggtaaaa cacaacagcg
      601 gtacggtagt gacaataccg gcggcgatca gcagtaaatt cagcgacatc gggttttgcc
      661 ccatatggct ggttgagctg tcggcaatag caaacaggta aattgccgcc acgggcagca
      721 gccacatggt ttcgattaac atgccggttt gcgcttcaac ggcaatcttc ttgcgtacca
      781 gaccgtagaa ggcaaaacta aatgccagcc ccagcgcgat aataggtagc gaaccaaaag
      841 tccacagctg gaccaacacg ccacatatcg ccagaatcac cgccagccat tgcatccggc
      901 ggaatcgctc gccgaggaaa atcatcccca gcacaatgtt caccagcggg ttaataaagt
      961 aaccaaggct cgcttccagc atatggtgat tgttcaccgc ccagataaac agtagccagt
     1021 tgccaccaat cagcacggca gagactgcca gcataaaaat tttctgtggc gtctgaatca
     1081 gcgtttttaa ataggaccac tggcggcaaa tgctcatcag caccaccata aagaaaaacg
     1141 accagatcac gcgatgcgtc aggatttcat cggcaggcac gtagtaaatc aacttgaagt
     1201 acgctggcgc tataccccaa ataaaataag cggcaagagc gagtaatacg ccctgccgcg
     1261 tttgttttgc atccatcggg aatactcatt tttaattggt aacagcagtt tacctgcttt
     1321 tatgtcttca acctaccata taggtggcgg tggcactggc aatataaagc tgttcttcat
     1381 tgtgtaattc aacgcgggcg acggcgactt tattgcctgc acgcaacagg ctactggtag
     1441 cagtaaaacg ctcgcccctg cctgggcgca gataatcaac gcgaagatca atggtcccca
     1501 tccgcgatag ccgctggcgt agttcatctt cactgatggt ttcgtggcgg gttaaggtac
     1561 ttcccacgca caccagaccg gcggcgacat ccagcgccga cgcaatgacc ccgccgtgca
     1621 aaatgctttg cgcccagttg cccaccatca ttggctgatt tttaaaggcc agctgtgcga
     1681 actctttttc gtaacgctcc agttccatcc ccaatgcgcg gttaaatggc atgtgataaa
     1741 caaacatctc acccactaat ttcagggctt gttcagcggt cagtacggca gacatatcat
     1801 ccttacactt cattggttaa tgaaatgttg attttatgct tctttgttgt tggtttctac
     1861 tttaggaagg gataactaac ggctatggag ttaagtatgt aaaatagccc gcagaaaaat
     1921 attcacctta tcaataattc gttacggaga acacgaccga tgcggactct gcagggctgg
     1981 ttgttgccgg tgtttatgtt gcctatggca gtatatgcac aagaggcaac ggtgaaagag
     2041 gtgcatgacg cgccagcggt gcgtggcagt attatcgcca atatgctgca ggagcatgac
     2101 aatccgttca cgctctatcc ttatgacacc aactacctca tttacaccca aaccagcgat
     2161 ctgaataaag aagcgattgc cagttacgac tgggcggaaa atgcgcgtaa ggatgaagta
     2221 aagtttcagt tgagcctggc atttccgctg tggcgtggga ttttaggccc gaactcggtg
     2281 ttgggtgcgt cttatacgca aaaatcctgg tggcaactgt ccaatagcga agagtcttca
     2341 ccgtttcgtg aaaccaacta cgaaccgcaa ttgttcctcg gttttgccac cgattaccgt
     2401 tttgcaggtt ggacgctgcg cgatgtggag atggggtata accacgactc taacgggcgt
     2461 tccgacccga cctcccgcag ctggaaccgc ctttatactc gcctgatggc agaaaacggt
     2521 aactggctgg tagaagtgaa gccgtggtat gtggtgggta atactgacga taacccggat
     2581 atcaccaaat atatgggtta ctaccagctt aaaatcggct atcacctcgg tgatgcggtg
     2641 ctcagtgcga aaggacagta caactggaac accggctacg gcggcgcgga gttaggctta
     2701 agttacccga tcaccaaaca tgtgcgcctt tatactcagg tttacagcgg ctatggcgaa
     2761 tcgctcatcg actataactt caaccagacc cgtgtcggtg tgggggttat gctaaacgat
     2821 ttgttttgat gaacggttga gtggttggca aatctggaat ccagcatcca ggattaccct
     2881 ctcagagact aaaagcattg cagtttctcg cgcaggcgct gaaaatagcg cctgttttta
     2941 tttcaggcaa tcggggtgaa tgtggcgcag gcggaagtgt tgaatctgga gtccggagct
     3001 aaacaggttt tacaagaaac ctttggctac caacagtttc gccccggcca ggaagaaatt
     3061 atcgacactg tgctttccgg tcgcgattgc ctggtcgtca tgcccaccgg tggcggaaaa
     3121 tccctttgct atcaaattcc tgccttattg ctaaacggcc ttaccgtggt tgtttcaccg
     3181 ctgatttcgt tgatgaaaga tcaggtggat caactgcaag ccaacggcgt ggcggcggcg
     3241 tgccttaact cgacgcaaac ccgcgaacag caacttgaag tgatgacagg ctgccgcacc
     3301 gggcaaattc gtctgcttta tatcgccccg gaacgcctga tgctggataa ctttcttgag
     3361 catctggcgc actggaatcc ggtgttatta gccgttgatg aagcgcactg tatctcccaa
     3421 tggggccacg atttccgccc ggaatatgcc gcgctcggtc agttgcgcca gcggttcccg
     3481 acgctgccgt ttatggcgct gaccgccaca gccgacgaca ccacgcgcca ggatatcgtg
     3541 cgcctgctgg ggctgaacga tccgctgatt caaatcagca gctttgaccg tccgaatatt
     3601 cgctacatgc tgatggagaa gttcaaaccg ctcgatcagt tgatgcgcta cgtgcaggaa
     3661 cagcgcggta agtccggcat tatctactgc aacagccggg cgaaagtaga agacaccgct
     3721 gcgcgcctgc aaagcaaggg tattagcgcg gcggcctatc atgccgggct ggaaaataat
     3781 gtccgcgccg acgtgcagga gaaattccag cgcgatgacc tgcaaattgt ggtggcgacg
     3841 gtggcgttcg gcatgggcat caataaacca aacgttcgct tcgtggtcca ctttgatatt
     3901 ccgcgcaata tcgaatccta ttatcaggaa accggtcgcg ccgggcgtga tggcctgccc
     3961 gcggaagcga tgctgtttta cgatccggcc gatatggcgt ggctgcgccg ttgtctggaa
     4021 gagaagccgc aggggcagtt gcaggatatc gaacgtcata aactcaatgc gatgggcgct
     4081 tttgccgaag cgcaaacttg ccgtcgtctg gtactgctga actattttgg cgaagggcgt
     4141 caggagccgt gcgggaactg cgatatctgc ctcgatccgc cgaaacagta cgacggttca
     4201 accgatgctc agattgccct ttccaccatt ggtcgtgtga atcagcgttt tggtatgggc
     4261 tatgtggtgg aagtgattcg cggcgctaat aaccagcgta tccgcgacta tggtcatgac
     4321 aaactaaaag tctacggtat gggccgtgat aaaagtcatg aacattgggt gagcgtgatc
     4381 cgccagctga ttcacctcgg tctggtgacg caaaatattg cccagcattc tgccctacaa
     4441 ctgacagagg ctgcgcgtcc ggtgctgcgc ggcgaatcct ctttgcaact tgccgtgccg
     4501 cgtatcgtgg cgctcaaacc gaaagcgatg cagaaatcgt tcggcggcaa ctatgatcgc
     4561 aaactgttcg ccaaattacg caaactgcgt aaatcgatag ccgatgaaag taatgtcccg
     4621 ccgtacgtgg tgtttaacga cgcaaccttg attgagatgg ctgaacagat gccgatcacc
     4681 gccagcgaaa tgctcagcgt taacggcgtt gggatgcgca agctggaacg ctttggcaaa
     4741 ccgtttatgg cgctgattcg tgcgcatgtt gatggcgatg acgaagagta gtcagcagca
     4801 taaaaaagtg ccagtatgaa gactccgtaa acgtttcccc cgcgagtcaa atgtatgttg
     4861 atgttatttc tcaccgtcgc catggtgcac attgtggcgc ttatgagccc cggtcccgat
     4921 ttcttttttg tctctcagac cgctgtcagt cgttcccgta aagaagcgat gatgggcgtg
     4981 ctgggcatta cctgcggcgt aatggtttgg gctgggattg cgctgcttgg cctgcatttg
     5041 attatcgaaa aaatggcctg gctgcatacg ctgattatgg tgggcggtgg cctgtatctc
     5101 tgctggatgg gttaccagat gctacgcggt gcactgaaaa aagaggcggt ttctgcacct
     5161 gcgccacagg tcgagctggc gaaaagtggg cgcagtttcc tgaaaggttt actgaccaat
     5221 ctcgctaatc cgaaagcgat tatctacttt ggctcggtgt tctcattgtt tgtcggtgat
     5281 aacgttggca ctaccgcgcg ctggggcatt tttgcgctga tcattgtcga aacactggcg
     5341 tggtttacag tcgttgccag cctgtttgcc ctgccgcaaa tgcgccgtgg ttatcaacgt
     5401 ctggcgaagt ggattgatgg ttttgccggg gcgttatttg ccggatttgg cattcatttg
     5461 attatttcgc ggtgatgcca gacgcgtctt cagagtaagt cggataaggc gtttacgccg
     5521 catccgacat tatttttcac gcatgcctcg ccgatgctaa cagcgctccc accagcataa
     5581 acaacgagcc gaaaatctta ttcagcgcct tcatctgctt tggtccttta atccatagag
     5641 caatccgttg agcaagggtg gcgtaaccga tcatcacaat aatatcgact acaatagtgg
     5701 tgacgccgag cacgatatac tgcatcagtt gcggctgctg tggcatgatg aattgcggaa
     5761 ttagcgccgc cagaaacaca atacttttgg gattggtgag attcacaaaa actgcgcgct
     5821 ggaacaaatg tcgacgcgat tgagtagagg ccagcgattt aaggtcaatt gcaccagcag
     5881 cgcgccactg ctggattccc agccaaatca agtaagccgc gcctgcccac ttcaacactt
     5941 caaacgcaat cactgagcgg gaaaatagcg tccccaaccc cacgccaacc agcacaatat
     6001 gaatcgccag tccggtctga agcccagcaa tagacgccac cgcgccgcga taaccgtggt
     6061 tgagcgaggt ggtcatagtg ttgattgcac cagagcctgg cgacaggctt aaaatgatcg
     6121 atgtcagcag gtaggcaaac caccattcta aggtcatgat gaactcccgg tgtgtctatt
     6181 tttgtgccac aatacgctac tgtcgcagcg ttgtgtcagg cacgctaaaa aaaacgattt
     6241 tacgtggttt aagaggcaga ttacccgatg tttcagcagc aaaaagactg ggaaacaaga
     6301 gaaaacgcgt ttgctgcttt taccatggga ccgctgactg atttctggcg tcagcgtgat
     6361 gaagcagagt ttactggtgt ggatgacatt ccggtgcgct ttgtccgttt tcgcgcacag
     6421 caccatgacc gggtggtagt catctgcccg gggcgtattg agagctacgt aaaatatgcg
     6481 gaactggcct acgacctgtt ccatttggag tttgatgtct taatcatcga ccatcgcggg
     6541 cagggacgtt ccggtcgcct gttagccgat ccgcatctcg ggcatgttaa tcgctttaat
     6601 gattatgttg atgatctggc ggcattctgg cagcaggagg ttcagcccgg tccgtggcgt
     6661 aaacgctata tactggcaca ttcgatgggc ggtgcgatct ccacattatt tctgcaacgc
     6721 catccaggtg tatgtgacgc cattgcgcta actgcgccaa tgtttgggat cgtgattcgt
     6781 atgccgtcat ttatggcacg gcagatcctc aactgggccg aagcgcatcc acgtttccgt
     6841 gatggctatg caataggcac cgggcgctgg cgcgcgttgc cgtttgctat caacgtactg
     6901 acccacagca gacagcgata tcgacgtaac ttacgcttct atgctgatga cccaacgatt
     6961 cgcgtcggtg ggccgaccta ccattgggta cgcgaaagta ttctggctgg cgaacaggtg
     7021 ttagccggtg cgggtgatga cgccacgcca acgcttctct tgcaggctga agaggaacgc
     7081 gtggtggata accgcatgca tgaccgtttt tgtgaactcc gcaccgccgc gggccatcct
     7141 gtcgaaggag gacggccgtt ggtaattaaa ggtgcttacc atgagatcct ttttgaaaag
     7201 gacgcaatgc gctcagtcgc gctccacgcc atcgttgatt ttttcaacag gcataactca
     7261 cccagcggaa accgctctac agaggtttaa atttcttatg taccaggttg ttgcgtctga
     7321 tttagatggc acgttacttt ctcccgacca tacgttatcc ccttacgcca aagaaactct
     7381 gaagctgctc accgcgcgcg gcatcaactt tgtgtttgcg accggtcgtc accacgttga
     7441 tgtggggcaa attcgcgata atctggagat caagtcgtac atgatcacct ccaacggtgc
     7501 gcgcgttcac gatctggatg gcaatctgat ttttgctcat aacctggatc gcgacattgc
     7561 cagcgatctg tttggcgtag tcaacgacaa tccggacatc attactaacg tttatcgcga
     7621 cgacgaatgg tttatgaatc gccatcgccc ggaagagatg cgctttttta aagaagcggt
     7681 gttcaaatat gcgctgtatg agcctggatt actggagccg gaaggcgtca gcaaagtgtt
     7741 cttcacctgc gattcccatg aacaactgct gccgctggag caggcgatta acgctcgttg
     7801 gggcgatcgc gtcaacgtta gtttctctac cttaacctgt ctggaagtga tggcgggcgg
     7861 cgtttcaaaa ggccatgcgc tggaagcggt ggcgaagaaa ctgggctaca gcctgaagga
     7921 ttgtattgcg tttggtgacg ggatgaacga tgccgaaatg ctgtcgatgg cggggaaagg
     7981 ctgcattatg ggcagtgcgc accagcgtct gaaagacctt catcccgagc tggaagtgat
     8041 tggtactaat gccgacgacg cggtgccgca ttatctgcgt aaactctatt tatcgtaatc
     8101 gttctttatt tggtcagttg tcaacctgat acttcgctac aatggatact cgttaatcaa
     8161 agagttttcc attgtggcgc tacttatcat caccacgatt ctgtgggcct tctcctttag
     8221 cttttatggc gagtaccttg cggggcacgt cgatagctat tttgcggtgc tggtgcgcgt
     8281 tggcctggcg gcactcgttt ttctgccgtt tctgcgtacc cgtggcaata gcctgaaaac
     8341 agtcggcctg tatatgctgg tgggcgcgat gcagctcggc gtgatgtata tgctgagttt
     8401 ccgcgcttat ctctacctga cggtttccga actgctgctg ttcaccgtac tgacgccgct
     8461 ctacatcacg ctgatttatg acatcatgag taagcgccgt ctgcgctggg gatatgcttt
     8521 tagtgccttg ttggcggtga ttggtgccgg gattattcgc tatgatcagg tcaccgacca
     8581 tttctggact ggcttgttgc tggtgcaact ctccaatatc acttttgcca tcggcatggt
     8641 gggttacaaa cgcctgatgg aaactcgccc gatgccgcag cataacgcct ttgcgtggtt
     8701 ctatcttggc gcgttcctgg tggcggtaat tgcatggttc ttgctgggaa atgcgcagaa
     8761 aatgccgcaa accacgttgc aatggggcat tctggtgttt cttggcgtgg tggcttccgg
     8821 gattggctac tttatgtgga actacggcgc gacgcaggtg gacgccggaa cgctgggcat
     8881 tatgaataat atgcacgttc cggcagggct gctggttaac ctggctatct ggcaccaaca
     8941 gccgcactgg ccaacgttta ttacaggcgc gctggtgagc ctggcctcac tgtgggtgca
     9001 tcgtaagtgg gtcgctccgc gctcttcaca aacggcagat gatcgcaggc gtgattgcgc
     9061 gctgagcgaa taaacgcttc cgtaactggc tgacgctgct cgccatcgcg cacggcggcg
     9121 tacagtcggc tccacaagcc ttcgcccagg gttttggtca ccaccagacc ctggcgctca
     9181 aaactctcta ctacccaatg cggtagcgcg gcaataccca tccgcgcggc aaccatctga
     9241 atcaacaata aggtgttatc gacgcttttc agtgacgggc tgacgcctgc cggctgaaga
     9301 aaatgccgcc agacatccag tcgactacgc tgcaccggat aaattaatag cgtctcgctg
     9361 gcgagatctt ccggtgtaat acgcgttttt gccgccagtg gatggtcagg tgctaacacc
     9421 aggcgtactt cataatcgaa catcggcgaa taatgcaggc cactgcgcgg caggatatcg
     9481 gaggtcatca ccagatccag ctcgccctgt tgcaacgcgg gctgcgggtc aaacgtcacg
     9541 cccgatttaa aatccatctc tacctgcggc cagttcttat ggaaattttc taacgcgggt
     9601 gtcagccact gaatacagct atggcactca atggcaatgc gcagacgcgt ctgctgcggt
     9661 tcattgcagg cttgtagtgc ctggctgatt tgcggcagca cctggtttgc cagttgcaac
     9721 aggatttctc cctgcggtgt aaagcgtagc ggctggctct tacgcacaaa tagccggaag
     9781 ccaaggcgtt gttccagatc gctaaactgg tgagacaggg cggattgcgt ctgatgcaac
     9841 gtcgccgcag cggctgcgag cgagccgcag ttccgcaacg cttgtagcgt tttcaggtgt
     9901 tttacttcga tcatgaaagt ccttcacttc ggcatgaata atttgcgctt gaggaatata
     9961 cagtaaccgc caattatgga tgtgtaaaca tctggacggc taaatactaa aaattccata
    10021 aggggcatat aatgacaatt cttaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc
    10081 gcgagctgaa aaaagcgcaa gaaagttatt gggcggggaa ctccacgcgt gaagaactgc
    10141 tgacagtagg gcgtgaactg cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg
    10201 acctgctgcc ggtgggcgat tttgcctggt acgatcatgt actgagcacc agtttgctgc
    10261 tgggtaatgt tccgccacgt catcagaaca aagacggttc ggtagatatc gacaccctgt
    10321 tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca
    10381 aatggtttaa caccaactat cactacatgg tgccggagtt cgttaaaggc caacagttca
    10441 aactgacctg gacgcagctg ctggaggaag tggacgaggc gctggcgctg ggccacaacg
    10501 ttaagcctgt gctgctgggg ccggttactt acctgtggct ggggaaagtg aaaggtgaac
    10561 agtttgaccg ccttagcctg ctgaacgaca ttctgccggt ttatcagcaa gtgctggcag
    10621 aactggcgaa acgcggcatc gagtgggtac agattgatga acccgcgctg gtactggaac
    10681 taccacaggc gtggctggac gcatacaaac ccgcttacga cgcgctccag ggacaggtga
    10741 aactgctgct gaccacctat tttgaaggcg taacgccaaa tctcgacacg attactgcgc
    10801 tgcctgttca gggtctgcat gttgacctcg tacatggtaa agatgacgtt gctgaactgc
    10861 acaagcgcct gccttctgac tggttgctgt ctgcgggtct gatcaatggt cgtaacgtct
    10921 ggcgcgccga tcttaccgag aaatatgcgc aaattaagga cattgtcggc aaacgtgatt
    10981 tgtgggtggc atcttcctgc tcgttgctgc acagccccat cgacctgagc gtggaaacgc
    11041 gtcttgatgc agaagtgaaa agctggtttg ccttcgccct acaaaaatgc catgaactgg
    11101 cactgctgcg cgatgcgctg aacagtggtg acacggcagc tctggcagag tggagcgccc
    11161 caattcaggc gcgtcgtcac tctactcgtg tacataatcc ggcggtagaa aagcgtctgg
    11221 cggcgatcac cgcccaggac agccagcgtg cgaatgtcta tgaagtgcgt gctgaagccc
    11281 agcgtgcacg ttttaaactg ccagcgtggc cgaccaccac gattggttcc ttcccgcaaa
    11341 ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg caatctcgac gccaataact
    11401 accgcacggg cattgcggaa catatcaggc aggccattgt tgaacaggaa cgtttgggac
    11461 tggatgtgct ggtacatggc gaggccgagc gtaatgacat ggtggaatac tttggcgagc
    11521 acctcgacgg gtttgtcttt acgcaaaacg gttgggtaca gagctacggt tcccgctgcg
    11581 tgaagccacc gattgttatt ggtgacgtta gtcgcccggc accgattacc gttgagtggg
    11641 cgaagtatgc gcaatctctg actgataaac cggtgaaagg gatgctgact ggcccggtga
    11701 ctattctctg ctggtcgttc ccgcgtgaag atgtcagccg tgaaaccatc gccaaacaga
    11761 ttgcgctggc gttgcgtgat gaagtggccg atctggaagc cgctggaatt ggcatcatcc
    11821 agattgacga accggcgctg cgcgaaggtt taccgctgcg tcgtagcgac tgggatgcgt
    11881 atctccagtg gggcgtagag gccttccgta tcaacgccgc cgtggcgaaa gatgacacac
    11941 aaatccacac tcacatgtgt tattgcgagt tcaacgacat catggattcg attgcggcgc
    12001 tggacgcaga cgtcatcacc atcgaaacct cgcgttccga catggagttg ctggagtcgt
    12061 ttgaagagtt tgattatcca aatgaaatcg gtccaggcgt ctatgacatt cactcgccaa
    12121 acgtaccgag cgtggaatgg attgaagccc tgctgaagaa agcggcaaaa cgcattccgg
    12181 cagagcgcct gtgggttaac ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc
    12241 gcgcggcact ggcgaacatg gtgcaggcgg cgcagaactt gcgtcggggg taaatccaaa
    12301 ccgggtggta ataccacccg gtcttttctc attacagcga cttcttccca ccatactgct
    12361 taaaccattc cagcatacgc tgccagccat ctttcgcaga ttcggcatga tagctcgggc
    12421 gataatcagc gttgaatgca tgcccggcat ccgggtacac gataatctct gctttcgcat
    12481 tagcagcccg cagcgcctgg cgcatggttt caacgctctc ctgcggaatg ctgttatcct
    12541 gaccaccata taagccgaga accggcgcgt taagatcggt tgcgatatca acaggttgtt
    12601 tcggtgaatt cagcgactta tcgcccgcca gtttgccgta ccacgccact gcggctttta
    12661 gctgtggatt atgcgcggca tacagccagg tgatacgtcc accccagcag aatccggtga
    12721 tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcgaca tgatcgagat
    12781 cggccagcac ctgcgagtca ggcacttttg ccaccagacc gctaagcaac gtgggaatat
    12841 cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcgata gccagatacc
    12901 cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca aaaatttcct
    12961 gcactacaat gaccactggc agtgggccat cgctttgctt tggtctggca tggtaagcag
    13021 gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg tcgtccgggg
    13081 tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgc aaatccagat tgttgtgttg
    13141 ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact atagtcagca
    13201 tgcaacaaat cacattgcct gaatcggctc atcttttatg cagtcctgca gaatgaagag
    13261 tgatttatgt gatttgaatc acttttggtg ggtaaattta tgcaacgcat ttgcgtcatg
    13321 gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg
    13381 actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc atctcggcct
    13441 cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc cggatcgtgt
    13501 ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc gcgaattcac
    13561 cacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgctctaccg gtatcggcgg
    13621 cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca ccttcctgcg
    13681 tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc tggttaccac
    13741 ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca ccgctggaat tcccggctgt
    13801 cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg aaatccattg gcgcgacaac
    13861 tcacgttggc gtgacagctt cttctgatac cttctaccca ggtcaggaac gttacgatac
    13921 ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc aggcgatggg
    13981 cgtaatgaac tatgaaatgg aatctgcaac cctgctgacc atgtgtgcaa gtcagggcct
    14041 gcgtgccggt atggtagcgg gtgttatcgt taaccgcacc cagcaagaga tcccgaatgc
    14101 tgagacgatg aaacaaaccg aaagccatgc ggtgaaaatc gtggtggaag cggcgcgtcg
    14161 tctgctgtaa ttctcttctc ctgtctgaag gccgacgcgt tcggcctttt gtatttttgc
    14221 gtagcgcctc gcaggaaatg cctttccaac tggacgtttg tacagcacaa ttctattttg
    14281 tgcgggtaag ttgttgcgtc aggaggcgtg gtggatttct caatcatggt ttacgcagtt
    14341 attgcgttgg tgggtgtggc aattggctgg ctgtttgcca gttatcaaca tgcgcagcaa
    14401 aaagccgagc aattagctga acgtgaagag atggtcgcgg agttaagcgc ggcaaaacaa
    14461 caaattaccc aaagcgagca ctggcgtgca gagtgcgagt tactcaataa cgaagtgcgc
    14521 agcctgcaaa gtattaacac ctctctggag gccgatctgc gtgaagtaac cacgcggatg
    14581 gaagccgcac agcaacatgc tgacgataaa attcgccaga tgattaacag cgagcagcgc
    14641 ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg agcacagcaa tcgccgggtt
    14701 gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc cgctacgtga acaactggac
    14761 ggtttccgcc gtcaggttca ggacagcttc ggtaaagaag cacaagaacg ccataccctg
    14821 acccacgaaa ttcgcaatct ccagcaactc aacgcgcaaa tggcccagga agcgatcaac
    14881 ctgacgcgcg cgctgaaagg cgacaataaa acccagggca actggggcga ggtagtattg
    14941 acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg aatatgaaac ccaggtcagc
    15001 atcgaaaatg acgcccgctc gcggatgcag ccggatgtca tcgtgcggct gccgcaggga
    15061 aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta ttttaacgcc
    15121 gaagacgact acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat
    15181 atccgtttgc tgggacgcaa agattatcaa cagctgccgg ggctgcgaac tctggattac
    15241 gtgctgatgt ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag
    15301 ctgatcaccg aagcgttgaa aaacaatatc atgctggtta gcccgactac gctgctggtg
    15361 gcgttgcgta ctattgccaa cctgtggcgt tatgagcatc aaagccgcaa cgcccagcaa
    15421 atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga tgacatgtcc
    15481 gcgattggtc aaagtctcga caaagcgcag gataactacc gccaggcaat gaaaaaaatc
    15541 tcttcaggtc gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggagtagaa
    15601 attaaacgcg agattaatcc ggatttggct gaacaggcgg tgagccagga tgaagagtat
    15661 cgacttcggt cggttccgga gcagccgaat gatgaagctt atcaacgcga tgatgaatat
    15721 aatcagcagt cgcgctagcc cattaggggt agttaagccg ggtagaaatc tagggcatcg
    15781 acgcccaatc tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga
    15841 taagtcacaa gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga
    15901 tatggtcgcc cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat
    15961 gtcatttggt attcatcgtt tgtggaagcg attcacgatt gattgcagcg gcgtacgccg
    16021 tgggcagacc gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg
    16081 cctggtcgga gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat
    16141 gggacgcgag aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa
    16201 cgctgaggcg ctgccgttcc cggataacac ctttgattgc atcaccattt cgtttggtct
    16261 gcgtaacgtc accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg
    16321 cggccgcctg ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta
    16381 tgatgcatac tccttccatg tgctgccgcg tattggctca ctggtcgcga acgacgccga
    16441 cagctaccgt tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc
    16501 catgatgcag gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt
    16561 ggcgctgcat cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag
    16621 tgacggcagg aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc gcgctgaaaa
    16681 cagcccgctc gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc ttttcgacgt
    16741 cattgattct ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg
    16801 ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat cgccagcagc
    16861 ttgccgccct gattcgcagt ggtgagctgg aagtgcaggg cgatattcag gtggtgcaaa
    16921 acttcgttgc gctggcagat ctggcagagt tcgaccctgc ggaactgctg gccccttata
    16981 ccggtgatat cgccgctgaa ggaatcagca aagccatgcg cggaggcgca aagttcctgc
    17041 atcacggcat taagcgccag caacgttatg tggcggaagc cattactgaa gagtggcgta
    17101 tggcacccgg tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg
    17161 ctgttgatgc cctgaccaaa cggctggaaa aactggaggc taaatgacgc caggtgaagt
    17221 acggcgccta tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc
    17281 caaaatgcgt atcaccctgc cgctacggct atggcgatac tcattattct ggatgccaaa
    17341 tcggcataaa gacaaacctt taggtgagcg actacgactg gccctgcaag aactgggacc
    17401 ggtatggatc aagttcgggc aaatgttatc aacccgccgc gatctttttc cgccgcatat
    17461 tgccgatcag ctggcgttat tgcaggataa agtcgctccg tttgatggca agctggcgaa
    17521 gcagcagatt gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga
    17581 aatcaagccg ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa
    17641 tggtaaagag gtggtgatta aagtcatccg cccggatatt ttgccggtca ttaaagcgga
    17701 tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg
    17761 tctgcgccca accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg aactgaattt
    17821 gctgcgggaa tctgccaacg ccattcagct tcggcgaaat tttgaagaca gcccgatgct
    17881 ctacatcccg gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat
    17941 ttacggtatt ccggtgtctg atgttgcggc gctggagaaa aacggcacta acatgaaatt
    18001 gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca
    18061 tgccgatatg caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata
    18121 tatcggcatt gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc
    18181 agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga
    18241 ttcaggctgg gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt
    18301 ctgtgaacct atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa
    18361 tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca
    18421 gaaaaccctg ctctatgtcg aaggggtagg acgccagctt tatccgcagc tcgatttatg
    18481 gaaaacagcg aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgcgct
    18541 ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga
    18601 attggtttac gacagtttgc gccagggcaa gtatttacag cacagtgttg ataagattgc
    18661 ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg
    18721 cgctacgtta gtattaagtg gcacattctt gttggtcagc cgacctgagt gggggctgat
    18781 gcccggctgg ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg
    18841 ctgatttttt catcgctcaa ggcgggccgt gtaacgtata atgcggcttt gtttaatcat
    18901 catctaccac agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc
    18961 cgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg
    19021 tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac
    19081 cagtcaggat gctgatttta ctgcgaaaac tatcgccgat aagcaggcgg atacgaatca
    19141 ggaacaggct aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt
    19201 tgatatcggt tttagcgaac tgctattggt gttcatcatc ggcctcgtcg ttctggggcc
    19261 gcaacgactg cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt tgcgttcact
    19321 ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa ctccaggagt ttcaggacag
    19381 tctgaaaaag gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat
    19441 ggatgaacta cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatcctga
    19501 aaaggcgagc gatgaagcgc acaccatcca taacccggtg gtgaaagata atgaagctgc
    19561 gcatgagggc gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg aacagaagcc
    19621 agaaaccacg ccagagccgg tggtaaaacc tgctgcggac gctgaaccga aaaccgctgc
    19681 accttcccct tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt
    19741 atcacgcatc tgattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg
    19801 atattcctgt gtctggtcta tttcgccaat gacatctatc acctggtatc cgcgccattg
    19861 atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt
    19921 acgccgatca agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat
    19981 caggtgtggg catttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg
    20041 ctgctggttt ccagctctct gctgttttat atcggcatgg cattcgccta ctttgtggtc
    20101 tttccgctgg catttggctt ccttgccaat accgcgccgg aaggggtgca ggtatccacc
    20161 gacatcgcca gctatttaag cttcgttatg gcgctgttta tggcgtttgg tgtctccttt
    20221 gaagtgccgg tagcaattgt gctgctgtgc tggatgggga ttacctcgcc agaagactta
    20281 cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg tcgggatgtt gctgacgccg
    20341 ccggatgtct tctcgcaaac gctgttggcg atcccgatgt actgtctgtt tgaaatcggt
    20401 gtcttcttct cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct
    20461 gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata
    20521 tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc
    20581 gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa tgggctactc atcaccggca
    20641 ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattcg tcctgttggt
    20701 caacggcggg cgtacatcct cacgacagca gccagtggca agctgcgact gaagaagcga
    20761 ttattgagct ggccgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta
    20821 accgcaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgctcag ctacgcattg
    20881 ccgcagaatt aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga
    20941 cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca
    21001 cacgcgaaga gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg
    21061 tttgcgatga acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga
    21121 aattgctgat cgaaactgat gcgccgtatc tgctcccgcg cgatctcacg ccaaagccat
    21181 catcccggcg caacgagcca gcccatctgc cccatatttt gcaacgtatt gcgcactggc
    21241 gtggagaaga tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa acactgtttg
    21301 ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat
    21361 taataagatt aagcaatagc atggagcgag cctcaccatc gggttcggtg aaaatggcct
    21421 gaaagccttc gaacgcgcct tcggtaataa tcaccttatc acccggataa ggggttgccg
    21481 gatcgacaat gtctttcggt ttatataccg atagctgatg aatcaccgcc gatgggacta
    21541 tcgctggcga cgcgccaaag cgcacgaagt ggctgacacc gcgggtcgcg ttgatagtcg
    21601 tggtatgaat cacttctgga tcaaattcga caaacaggta gttgggaaac aatggctcac
    21661 tgactgcagt acgttttcca cgcacgattt tttccagggt gatcatcggt gccaggcaat
    21721 tcacagcctg tctttcgagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca
    21781 gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa
    21841 agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta
    21901 atttaacaaa tttacagcat cgcaaagatg aacgccgtat aatgggcgta gattaagagg
    21961 ctacaatgga cgccatgaaa tataacgatt tacgcgactt cttgacgctg cttgaacagc
    22021 agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg
    22081 ctgaccgcac tttgcgtgcc ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact
    22141 caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatggggc
    22201 aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg
    22261 agccgccaaa aggtttccgc gacctgtttg ataaactgcc gcagtttaag caagtattga
    22321 acatgccgac aaagcggctg cgtggtgcgc cctgccaaca aaaaatcgtc tctggcgatg
    22381 acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc gcaccgctga
    22441 ttacctgggg gctgacagtg acgcgcggcc cgcataaaga gcggcagaat ctgggcattt
    22501 atcgccagca gctgattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg
    22561 gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg
    22621 tggcgctggg tgccgatccc gccacgattc tcggtgcagt cacccccgtt ccggatacac
    22681 tttcagagta tgcgtttgcc ggattgctgc gcggtaccaa gaccgaagtg gtgaagtgta
    22741 tctccaatga ccttgaagtg cccgccagtg cggagattgt gctggaaggg tatatcgaac
    22801 aaggcgaaac tgcgccggaa gggccgtatg gcgaccacac cggttactat aacgaagtcg
    22861 atagtttccc ggtatttacc gtgacgcata ttacccagcg tgaagatgcg atttaccatt
    22921 ccacctatac cgggcgtccg ccagatgagc ccgcggtact gggcgtggcg ttgaacgaag
    22981 tgtttgtacc gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg
    23041 aaggctgttc ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc ggacacgcga
    23101 agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga
    23161 tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacca
    23221 cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg
    23281 attttgcctc gcctgtctcc gggctgggtt caaaaatggg gctggatgcc acgaataaat
    23341 ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtcg
    23401 cgcatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat
    23461 gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct
    23521 gtaaagtgac ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc atcgtgccag
    23581 acgcggcctt ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca
    23641 aacgtccgtt ctcaatggcc tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg
    23701 gcgcttctga aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagatcatc
    23761 aaatcgtggt cgatattccc cacggagaag cgtggctgcg cgatgatgaa gagcgtccga
    23821 tgattttgat tgcgggcggc accgggttct cttatgcccg ctcgattttg ctgacagcgt
    23881 tggcgcgtaa cccaaaccgt gatatcacca tttactgggg cgggcgtgaa gagcagcatc
    23941 tgtatgatct ctgcgagctt gaggcgcttt cgttaaagca ccctggtctg caagtggtgc
    24001 cggtggttga acaaccggaa gcaggctggc gtgggcgtac tggtaccgtg ttaacggcgg
    24061 tattgcagga tcatggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga
    24121 tggcgaaaat tgcccgtgac ctgttttgca gtgagcgtaa tgcgcgggaa gatcgcctgt
    24181 ttggcgatgc gtttgcattt atctgagata taaaaaaacc cgcccctgac aggcgggaag
    24241 aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg gcaattggtg
    24301 cacgacgcct gatgcgacgc tggcgcgtct tatcaggcct acattggtgc cggatcggta
    24361 gaccggataa ggcgttcacg ccgcatccgg caagtggtta aacccgctca aacaccgtcg
    24421 caataccctg acccagaccg atacacatcg tcgccagacc aaactgaacg tctttgcgtt
    24481 ccatcagatt cagcagcgtg gtgctgatac gcgcaccgga acagcccagc ggatgaccca
    24541 gcgcgatcgc accaccattg aggttgatct tctcgtcaat ctgctccatt agcccgagat
    24601 ctttaatgca cggcaggatc tgcgcggcaa aggcttcgtt catctcaaac acgccgatat
    24661 cgctggcaga aagccccgct tttttcagcg ccagcttcga ggccggaacc gggccgtaac
    24721 ccataatcga tgggtcacaa ccaacgaccg ccatcgaacg cacacgagcg cgcggcttaa
    24781 gacctaattc atgggcgcgg ctttcactca tcaccagcat ggcagctgcg ccatcggaaa
    24841 gtgcagaaga tgtgcccgcc gttaccgtac cgtttactgg atcaaacgcc ggacgcagcg
    24901 tggcgagggc ttccacggtg gtttccgggc gaatcacttc gtcgtaatta aactgcttca
    24961 ggacgccgtc ggcatcgtga ccaccagttg ggatgatttc atttttaaat gcggccgact
    25021 gcgtggcggc ccaggcgcgg gcgtgtgacc gcgcggcaaa ggcatcctgc atttcacggc
    25081 tgataccgtg catacgcgcc agcatttctg ccgttaagcc catcatgccc gccgctttgg
    25141 cgacattgcg gctcaggccg gggtgaaaat cgacgccgtg actcatcggc acatggccca
    25201 tatgctccac gccgccaacc agacatgcct gcgcatcgcc agtcatgatc attcgtgctg
    25261 cgtcatgcag tgcctgcatg gatgaaccac acaagcgatt gacggtaacc gccgggacag
    25321 agtgcggcac ttctgccagc agcgccgcgt tacgggcgat attaaaaccc tgctccagcg
    25381 tctgctgcac acaaccccag taaatatcgt cgagggccgc cgcttccagc gccgggttac
    25441 gcgccagcag gctacgcatt aaatgagcgg agagatcttc tgcacgcacg ttacgaaaag
    25501 caccgccctt cgaacggccc atcggggtgc gaattgcatc gacaatgaca acctgttcca
    25561 ttgtgactcc ttaagccgtt ttcaggtcgc caaccggacg ggctggctca accggaggat
    25621 agtacggttc gttatgacgc gctttattac gcagaccttc cggcacttca tacagcgggc
    25681 cgaggtgctg atattgctgt gccatatcga ggtattttgc gctaccgagg gtgtccagcc
    25741 agcggaacgc gccgccgtgg aacggaggga agcccaggcc gtagaccagc gccatatccg
    25801 cttccgccgg agtggcgata atgccttcct ccagacagcg caccacttcg ttgaccatcg
    25861 ggatcatcat gcgggcgata atctcttctt cgctgaaatc gcgcttcggc tggctcactt
    25921 ctgccagcag gtcttcaacg gcggtgtctt cttctttctt cagcttacct ttgctgtctt
    25981 ctttataacg ccagaaaccg aggccgttct tctgaccaaa gcggttggca tcaaacagcg
    26041 cgtcgatggc atcgcggtaa tctttctgca tccgctgcgg gaagcctgct gccatgacag
    26101 cctgagcgtg atgcgcggta tcaatgccca cgacatccag cagatatgcc ggacccatag
    26161 gccagccaaa ctgtttttcc atcactttgt cgatcttgcg gaaatccgcg ccgtcgcgca
    26221 gcagctggct gaaaccggcg aaatacggga acagcacgcg gttaacaaag aagccggggc
    26281 agtcgttaac cacaatcggc gtcttgccca tcttgctcgc ccaggcgaca actttcgcga
    26341 tggtttcgtc ggagcttttc tcgccgcgaa taatttctac caacggcatc cggtgtaccg
    26401 ggttaaaaaa gtgcattccg cagaagtttt ccgggcgttc cagcgcgttg gccagttcgc
    26461 tgataggaat ggttgaagtg ttagacgcca gcacggtatc cgggcgtact ttttgttcgg
    26521 tttctgccag tacggctttt ttcactttcg ggttttcgac aaccgcttct accacaacat
    26581 ccacgcggtc aaacccggcg tagtcgagcg ttgggtggat tgtggagatc acgccagcca
    26641 gtttcagacc atcgatcttg ccgcgctcaa gctgcttgtt cagcagtttc gcggcttcgg
    26701 tcatgccgag ggttaacgac ttgtcgttga tatctttcat gacaaccggc acgcctttcc
    26761 acgcagactg gtaagcgatg ccgccgccca taatgcctgc acccagcacc gcggcctgtt
    26821 tcggggtttc aacgtctttg gtgagtttct tcgctttgcc ttttacatat tgatcgttaa
    26881 ggaaaatgcc gaccagtgcg cgggcttcgt tggtatgcgc cagcgggaca aaacttttgt
    26941 tttccaggtt taaggcttct tcacgaccaa aacgggccgc agcttcaatg gtttttactg
    27001 cggtgatggg ggccggataa tgtttccccg ctgtttgtgc gaccatccct ttagcgatgg
    27061 tgaagctcat ggtggcttca atcttgctca gttttagtgg ttccagcttc ggctgacgtt
    27121 ttgctttcca gtcgaggtcg ccgttaatgg cctggcgtaa aaccgccttt gcgccttcaa
    27181 ccagtttttc tgctttgact acgccatcca ccagaccgat tttcagcgcc tgatccgcgc
    27241 cgacatcttt accggcggca atgatttcca gcgcactgtc agcgcccagc atacgtggca
    27301 tacgtacaga accgccaaag cccggcatga tgcccagttt ggtttccggc agaccgatgc
    27361 gcagatccgg cgtcgccaga cgataatcgg tcgccagcac gcattcgcag ccaccgccca
    27421 gcgcatagcc gttgacggca gcaatggtcg gcaccggcag atcttccagg cgattaaaca
    27481 cgctattggc aaagtgcagc cactgactta actgttcttc aggaacgagg aacagggaca
    27541 aaaattcggt gatatcagca ccgacgataa aggctgcttt gttcgaacgc agcagcagcc
    27601 cttttagatc tgattgctgt tccagcacgc cgatggcctc gccgaggctg gcgacggtcg
    27661 cagtgtcgag tttattaact gaacctgggg catcaaatac cagttcggca atgccatctt
    27721 ccagccagtc aaggtacagg gtgtcgcctt tgtaaagcat gtcagtctcc tgaatccgca
    27781 aggtgatctg gtcgtaccag atgcatcgaa gtgtgtattt tgtgttaaaa atatgcaaat
    27841 aaaagattaa agaaatgccg atctgatcac gctcggcaga aatcacgctc tggatgaacg
    27901 atgtgctaag atgcggagac ttaaggtcaa aaaaacagaa gggtaaaaaa tggaatcact
    27961 ggcctcactc tataaaaatc atatagctac cttacaggaa cggactcgcg atgcgctggc
    28021 gcgcttcaag ctggatgcgt tacttattca ctccggcgaa ctgttcaacg tttttctcga
    28081 cgatcatccc tatcctttta aagtgaaccc gcaattcaaa gcgtgggtgc cggtaactca
    28141 ggtgccaaac tgctggctgc tggtggatgg cgtgaacaag ccgaaactgt ggttctatct
    28201 gccggttgat tactggcaca acgtcgaacc gctgccgacc tctttctgga ctgaggatgt
    28261 ggaagtgatc gcgctgccga aagccgatgg cattggtagc ctgctgcctg ctgcgcgcgg
    28321 caatatcggt tatatcggtc cggtgccgga acgtgcgctg caactgggta ttgaggccag
    28381 caacatcaac ccgaaagggg tgatcgacta cctacattac tatcgctcct tcaaaaccga
    28441 gtacgaactg gcctgtatgc gtgaagcgca gaaaatggcg gtcaacggtc atcgcgcggc
    28501 agaagaagcg ttccgttctg gcatgagcga gttcgatatc aacattgcct atctgaccgc
    28561 gaccggtcat cgtgataccg acgtacctta cagcaacatt gtggcgctta acgaacacgc
    28621 tgcggtgctg cattacacca aactggacca ccaggcaccg gaagagatgc gcagcttcct
    28681 gctagatgcc ggggccgaat ataacggcta tgcggctgac ctgacccgta cctggtcggc
    28741 aaaaagtgac aacgactacg cacagctggt gaaggacgtt aatgatgaac aactggcgct
    28801 gatcgctacc atgaaagcag gcgtcagcta tgtggattac cacatccagt tccatcagcg
    28861 catcgccaaa ctgctgcgta aacatcaaat catcaccgat atgagtgaag aagcgatggt
    28921 cgaaaacgat ctcaccggac cgtttatgcc gcacggtatc ggtcatccgc tgggcctgca
    28981 ggtgcatgac gtagccggtt ttatgcagga tgatagcggt acacacctcg cggcaccggc
    29041 aaaatatccg tacctgcgct gcacccgtat tctccagccg ggtatggtgt taaccatcga
    29101 accgggtatc tacttcattg aatcgctact ggcaccgtgg cgtgaagggc agttcagcaa
    29161 gcacttcaac tggcagaaaa ttgaagcact gaaaccgttc ggcggcattc gtatcgaaga
    29221 caacgtggtg atccacgaaa acaacgtgga aaacatgacc cgggatctga aactggcgtg
    29281 atggaaagct ggttaattcc tgcggcaccg gtcacggtcg ttgaagagat caaaaagagc
    29341 cgcttcataa cactgttggc gcataccgat ggcgttgagg cggcgaaagc gtttgttgaa
    29401 tcggtgcggg cagaacaccc cgatgcccgc caccattgcg tggcgtgggt ggcgggtgcg
    29461 ccggatgatt ctcaacagct gggtttctct gacgacgggg agccggcagg aacggcaggt
    29521 aaaccgatgc tcgcccagct aatgggcagc ggcgtcgggg aaattaccgc tgtggtagtg
    29581 cgctactacg gtggcatatt gcttggcacc ggtggggtag tgaaagcgta tggcggcggc
    29641 gtgaatcagg cgctgcgcca gctaacgacc caacgcaaga cgccattaac cgaatatact
    29701 ttgcaatgtg aatatagtca gttaaccggc attgaagcgt tgctggggca gtgtgacggc
    29761 aaaattatca acagtgatta tcaggcattc gttctgctgc gggtggcgct tccggcggcg
    29821 aaagtggctg aattttccgc aaagctggcg gattttagcc gtggttcatt gcaattgtta
    29881 gcgattgaag aataatcccc acttcgtttt gcagaactaa ggaagcggca gagatgcatt
    29941 ttcgcgccat tacccgaatc gttggactac tggtcatctt attttcaggg accatgatta
    30001 tccctgggct ggtagcactc atctaccggg atggagcggg ccgcgctttt acccagacct
    30061 tttttgtcgc cctcgccatt ggttctatgc tgtggtggcc gaaccgtaaa gagaagggcg
    30121 aactgaaatc ccgtgagggg tttctgatag tggtgctgtt ctggaccgtg ctgggtagcg
    30181 tcggtgcgct cccttttatc ttctcggaaa gcccgaacct cacgattacc gatgcgtttt
    30241 ttgaatcttt ctctggcctg accaccaccg gggccactac gctggtgggg ctggattcgc
    30301 tccctcatgc catcctcttt tatcgccaga tgctgcaatg gtttggcggg atggggatca
    30361 tcgtgttagc ggttgcgata ctgcctatcc tcggcgtggg tgggatgcag ctctatcgcg
    30421 cagaaatgcc cggcccgctg aaagataaca aaatgcgccc gcgaattgcg gaaacggcga
    30481 aaaccctgtg gttgatttat gtcttgctga ccgtcgcctg tgcgctggcg ttgtggtttg
    30541 ccggaatgga tgcctttgat gccatcggcc atagctttgc gactatcgct attggcggct
    30601 tctcgacaca tgatgccagt atcggttatt tcgacagccc gactattaac actatcattg
    30661 ctatcttcct gctgatctcc ggctgtaact acggtctgca cttttcactg ttaagtgggc
    30721 gtagtctgaa ggtttattgg cgcgatccgg aatttcgcat gtttatcggc gtacagttta
    30781 cgctggtggt tatttgtaca ctcgtactgt ggtttcataa tgtctacagt tcggcgctga
    30841 tgacaattaa ccaggcgttt ttccaggtgg tatcgatggc gacaaccgcc gggtttacga
    30901 ctgacagcat tgcccgctgg ccgctctttt tgccggtact gcttttatgt tcagctttta
    30961 tcggcggttg cgccgggtca acgggcggtg gcctgaaagt gatccgcatc ctgctgctgt
    31021 ttaagcaggg gaaccgtgag ctgaaacgac tggtgcatcc gaacgccgtc tatagcatta
    31081 agctggggaa tcgcgcactg ccggaacgta tcctcgaagc cgtttgggga tttttctccg
    31141 cctatgcatt ggtgtttatt gtcagtatgc tggcgattat cgccacgggc gtggatgact
    31201 tttctgcctt tgcctcagtt gttgcgacat tgaataacct ggggccaggg cttggcgtgg
    31261 ttgctgataa ctttaccagt atgaacccgg tggctaaatg gatcctgatt gccaacatgc
    31321 tgtttggtcg tctcgaggtc tttacattgc tggtgctctt taccccgact ttctggcgtg
    31381 aatgatggag taatacgtga aaacattaat tcttttctca acaagggacg gacaaacgcg
    31441 cgagattgcc tcctacctgg cttcggaact gaaagaactg gggatccaga cggatgtcgc
    31501 caatgtgcac cgcattgaag aaccacaatg ggaaaattat gaccgtgtgg tcattggtgc
    31561 ttctattcgc tatggtcact accactctgc gttccaggaa tttgtcaaaa aacatgcgac
    31621 gcgactgaat tcgatgccga gcgcctttta ttccgtaaac ctggtggcgc gcaaaccgga
    31681 gaagcgtact ccacagacca acagctacgc gcggaagttt ctgatgaact cgcaatggcg
    31741 tcccgatcgc tgcgcggtca ttgccggggc gctgcgttac ccacgttatc gctggtacga
    31801 ccgttttatg atcaagctga ttatgaagat gtcaggcggt gaaacggata cgcgcaaaga
    31861 agttgtctat accgattggg agcaggtggc gaatttcgcc cgagaaatcg ctcatttaac
    31921 cgacaaaccg acgctgaaat aagcacaaag aa
//
LOCUS       DAZKHC020000050        31009 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZKHC020000050 DAZKHC020000000
VERSION     DAZKHC020000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.050"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            248..631
                     /gene="secE"
                     /locus_tag="ACHOSM_RS13185"
                     /old_locus_tag="pgaptmp_002637"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="ACHOSM_RS13185"
                     /old_locus_tag="pgaptmp_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGU4559132.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="ACHOSM_RS13190"
                     /old_locus_tag="pgaptmp_002638"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="ACHOSM_RS13190"
                     /old_locus_tag="pgaptmp_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGU4559133.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="ACHOSM_RS13195"
                     /old_locus_tag="pgaptmp_002639"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="ACHOSM_RS13195"
                     /old_locus_tag="pgaptmp_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGU4559134.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="ACHOSM_RS13200"
                     /old_locus_tag="pgaptmp_002640"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="ACHOSM_RS13200"
                     /old_locus_tag="pgaptmp_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGU4559135.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHOSM_RS13205"
                     /old_locus_tag="pgaptmp_002641"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHOSM_RS13205"
                     /old_locus_tag="pgaptmp_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGU4559136.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="ACHOSM_RS13210"
                     /old_locus_tag="pgaptmp_002642"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="ACHOSM_RS13210"
                     /old_locus_tag="pgaptmp_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGU4559137.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHOSM_RS13215"
                     /old_locus_tag="pgaptmp_002643"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHOSM_RS13215"
                     /old_locus_tag="pgaptmp_002643"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGU4559138.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHOSM_RS13220"
                     /old_locus_tag="pgaptmp_002644"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHOSM_RS13220"
                     /old_locus_tag="pgaptmp_002644"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGU4559139.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12676..13131
                     /locus_tag="ACHOSM_RS13225"
                     /old_locus_tag="pgaptmp_002645"
     CDS             12676..13131
                     /locus_tag="ACHOSM_RS13225"
                     /old_locus_tag="pgaptmp_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HGU4559140.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPHF"
     gene            complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="ACHOSM_RS13230"
                     /old_locus_tag="pgaptmp_002646"
     CDS             complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="ACHOSM_RS13230"
                     /old_locus_tag="pgaptmp_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HGU4559141.1"
                     /translation="MKTFSDRWRQLDWDDIRMRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14756..15526)
                     /gene="thiG"
                     /locus_tag="ACHOSM_RS13235"
                     /old_locus_tag="pgaptmp_002647"
     CDS             complement(14756..15526)
                     /gene="thiG"
                     /locus_tag="ACHOSM_RS13235"
                     /old_locus_tag="pgaptmp_002647"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HGU4559142.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="ACHOSM_RS13240"
                     /old_locus_tag="pgaptmp_002648"
     CDS             complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="ACHOSM_RS13240"
                     /old_locus_tag="pgaptmp_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HGU4559143.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="ACHOSM_RS13245"
                     /old_locus_tag="pgaptmp_002649"
     CDS             complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="ACHOSM_RS13245"
                     /old_locus_tag="pgaptmp_002649"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HGU4559144.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="ACHOSM_RS13250"
                     /old_locus_tag="pgaptmp_002650"
     CDS             complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="ACHOSM_RS13250"
                     /old_locus_tag="pgaptmp_002650"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGU4559145.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="ACHOSM_RS13255"
                     /old_locus_tag="pgaptmp_002651"
     CDS             complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="ACHOSM_RS13255"
                     /old_locus_tag="pgaptmp_002651"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HGU4559146.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19071..19171)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="ACHOSM_RS13260"
                     /old_locus_tag="pgaptmp_002652"
     CDS             complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="ACHOSM_RS13260"
                     /old_locus_tag="pgaptmp_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HGU4559147.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19795..20568
                     /gene="nudC"
                     /locus_tag="ACHOSM_RS13265"
                     /old_locus_tag="pgaptmp_002653"
     CDS             19795..20568
                     /gene="nudC"
                     /locus_tag="ACHOSM_RS13265"
                     /old_locus_tag="pgaptmp_002653"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HGU4559148.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20608..21672
                     /gene="hemE"
                     /locus_tag="ACHOSM_RS13270"
                     /old_locus_tag="pgaptmp_002654"
     CDS             20608..21672
                     /gene="hemE"
                     /locus_tag="ACHOSM_RS13270"
                     /old_locus_tag="pgaptmp_002654"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HGU4559149.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21829..22500
                     /gene="nfi"
                     /locus_tag="ACHOSM_RS13275"
                     /old_locus_tag="pgaptmp_002655"
     CDS             21829..22500
                     /gene="nfi"
                     /locus_tag="ACHOSM_RS13275"
                     /old_locus_tag="pgaptmp_002655"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HGU4559150.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22543..23133
                     /gene="yjaG"
                     /locus_tag="ACHOSM_RS13280"
                     /old_locus_tag="pgaptmp_002656"
     CDS             22543..23133
                     /gene="yjaG"
                     /locus_tag="ACHOSM_RS13280"
                     /old_locus_tag="pgaptmp_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HGU4559151.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23320..23592
                     /gene="hupA"
                     /locus_tag="ACHOSM_RS13285"
                     /old_locus_tag="pgaptmp_002657"
     CDS             23320..23592
                     /gene="hupA"
                     /locus_tag="ACHOSM_RS13285"
                     /old_locus_tag="pgaptmp_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HGU4559152.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23605..24300
                     /gene="yjaH"
                     /locus_tag="ACHOSM_RS13290"
                     /old_locus_tag="pgaptmp_002658"
     CDS             23605..24300
                     /gene="yjaH"
                     /locus_tag="ACHOSM_RS13290"
                     /old_locus_tag="pgaptmp_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HGU4559153.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGVVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24302..24727)
                     /gene="zraP"
                     /locus_tag="ACHOSM_RS13295"
                     /old_locus_tag="pgaptmp_002659"
     CDS             complement(24302..24727)
                     /gene="zraP"
                     /locus_tag="ACHOSM_RS13295"
                     /old_locus_tag="pgaptmp_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HGU4559154.1"
                     /translation="MKRNTKIALVMMVLSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            complement(24730..24858)
                     /locus_tag="ACHOSM_RS13300"
                     /old_locus_tag="pgaptmp_002660"
     CDS             complement(24730..24858)
                     /locus_tag="ACHOSM_RS13300"
                     /old_locus_tag="pgaptmp_002660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559155.1"
                     /translation="MTRYPQQASHFYSFIMPDYPSYQRFIVGTEDAILEVRQPLED"
     gene            24965..26341
                     /gene="zraS"
                     /locus_tag="ACHOSM_RS13305"
                     /old_locus_tag="pgaptmp_002661"
     CDS             24965..26341
                     /gene="zraS"
                     /locus_tag="ACHOSM_RS13305"
                     /old_locus_tag="pgaptmp_002661"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HGU4559156.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26338..27663
                     /gene="zraR"
                     /locus_tag="ACHOSM_RS13310"
                     /old_locus_tag="pgaptmp_002662"
     CDS             26338..27663
                     /gene="zraR"
                     /locus_tag="ACHOSM_RS13310"
                     /old_locus_tag="pgaptmp_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HGU4559157.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27660..28949)
                     /gene="purD"
                     /locus_tag="ACHOSM_RS13315"
                     /old_locus_tag="pgaptmp_002663"
     CDS             complement(27660..28949)
                     /gene="purD"
                     /locus_tag="ACHOSM_RS13315"
                     /old_locus_tag="pgaptmp_002663"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGU4559158.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWNERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28961..30550)
                     /gene="purH"
                     /locus_tag="ACHOSM_RS13320"
                     /old_locus_tag="pgaptmp_002664"
     CDS             complement(28961..30550)
                     /gene="purH"
                     /locus_tag="ACHOSM_RS13320"
                     /old_locus_tag="pgaptmp_002664"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGU4559159.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7326 a         8032 c         8461 g         7190 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct tgacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 acgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacgccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgtcgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caagaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gccgaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgtt ctgggtctgt actatatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgcaaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgtgatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atggggggag tttagggaaa gagcatttgt
    12541 cagaatattt aaggaatttc tgaatactca taatcaatgt agagatgact aatatcctga
    12601 aactgactga actaattgag tcaaactcgg caaggattcg atactattcc tgtgtaactt
    12661 tcttaaggaa cgagaatgaa acaggaagtg gaaaagtggc gaccttttgg acatccggat
    12721 ggtgatattc gtgatttatc atttcttgat gctcatcagg ctgtctacgt tcagcatcat
    12781 gagggcaaag agcctttaga gtatcgcttt tgggttacct actctcttca ctgcttcaca
    12841 aaagattatg aacatcagac gaacgaagaa aaacaatcgt taatgtacca cgcgcctaaa
    12901 gaatctcgtc ccttctgcca gcaccgttat aacttagcgc gcacacactt aaaaagaact
    12961 attttggcgc tgccagaaag caacgttatt catgccgggt atggtagcta tgccgtgatt
    13021 gaggtggact tagacggagg agataaggca ttttactttg ttgcgttcag ggctttcagg
    13081 gaaaagaaaa aactccgttt gcatgtaact agcgcttatc cccatttctg aaaaacagaa
    13141 aggtaaatca gtgaaatttt tcaccattgc ctacaactta ttgagaaata agcagcttcc
    13201 tcagccctca aaataacaaa acccacctta aggtgggttt cgccagagaa ttatctctgg
    13261 tattcagaac gccattaccg gactttgcct tgaccttgcg ataatcgcag gttgcgggat
    13321 gtctgaattt cttcagtctg ctgcatcctg gaagatgaga acatgtgttc ttattttcgt
    13381 ctctatcata gttgagtatt tactctctta caatcagatc tctttcattg ctcaacaggc
    13441 gatggcttca gactttgcat tacggaattt ttaagaaagg cagggcgaaa cgaggaagaa
    13501 gcttttcgcc ctggacaaca ttcctgctga cggcactacc ataaaaaaac acaacgcttt
    13561 caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg ttcttacatt
    13621 ccgtctcata gtctttgcga ggcccgtccc agatagctgt cccagtcttt ccataccggc
    13681 tgcaaaccct gagcggttaa cgcggcagca accgcttccg gtctgcgatc gtcgtgcggt
    13741 gagaactgtt ccaactcggg gtgattatcg gcatagccac ctggctgcgt tttcgagaag
    13801 gcgctgacgt tattgatcgc cagcggaata acgcgatcgc gaaaccacgg tgattcccgc
    13861 gtggagagtg acagttcaat ctccggtgca agcagtcgga aggcgcagat ggtttgcact
    13921 aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacacgg gcgcaggcgc
    13981 ggaaaggaga cagagtaacg gctttgccag taatgctgtt gcagccatag caaatgttcg
    14041 gcaaccatat agcagtcaac gcgccagttg tcggaaaggc caattagcgc gccgaggcct
    14101 atcttatcaa tccccgcacg ccccagccga tccggcgttt ccagccgcca gaagaagtcc
    14161 tgttttttgc ctttcagatg atggcgggca taagtcgcct cgtgatatgt ctcctgataa
    14221 accatcacgc catccagacc aagctgcttt aactcggcgt attccgtctc cgccagcggt
    14281 tgcacttcca tttgtagtga agagaactgt tcacgcaggg cggggagatg acgacgaaag
    14341 taatccatcc ccactttcgc ctgatgttca ccagtgacta atagcagatg ttcaaagccc
    14401 atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt tttgcgcttg
    14461 atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag attggaaaga
    14521 taaagcggga cgtagaaact aactgtgttg ccaaatcgct gacgggtcag acgctgcgcc
    14581 cgttgggcca gttgttccag atagccactg gcggctggcg ataacagcgc catcatatcg
    14641 tcgcgggtga attgcgaggc atttagcgcc cgctctacgt caacagccgt tttgccgttg
    14701 atacgcatgc ggatgtcatc ccagtccagt tgtcgccagc gatcgctgaa ggttttcatg
    14761 ccgatgcctc cagaaatcca gtcagcgggc tggtggcatg agcaaaatga ctgcggctgc
    14821 ccggtccgga ctgacgtgcc agcaggcctg cttctaccgc cagacgaaat gccttcgcca
    14881 tgttgacggg atcgtccgct acggcaatcg ccgtattcac taacaccgcg tcggccccca
    14941 tttccagggc ctgcgcggca tggctgggaa cgccgatgcc agcatcgaca accaccggaa
    15001 ctgtggcctg ctggataata atctccagca tggcacgggt ttccagtccc tgattcgagc
    15061 caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga cgtttacaca
    15121 ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc agcatttcgg
    15181 cggctttcag agtttcgatg ggatcgggca acagccagcg ggcgtcaggg tgaatctcta
    15241 attttaacca gtttgtgcct aacgcttcac gtgccagatg ggcggcgaaa atggcttctt
    15301 ccgctgtttt cgccccggaa gtatttggca gcagggtcac acccgccgcg ataagcggtt
    15361 cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt catcgccagt gtcaccagct
    15421 ggctgccgga agcgcggatc gcctccacca tcagttgtga agaagcgaat ttccctgtgc
    15481 cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca accccctgca
    15541 ataacctgaa aaagcaggat ctggtcgcca tcctgcacga tatgttgcgt ccactgctca
    15601 cgcgggacga tttgctgatt gatcgccaga gccgcgcccg cttgtcgttg gtcgagttgc
    15661 tccagtagtt cgtgaacagt ttgcccggcg gcgcactgca tcggttgatc gttaaacagg
    15721 atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg ccaggctgcg
    15781 ccactggctc gatttaccgt cgaacagtcg gagttctccc gcaggtgtct ctataccgct
    15841 taataactta atggcttcca gtgcctgcaa agtgcccata accccgacca ccgggccaac
    15901 cacgcccgcc gtgcggcagt tgcgttctgg ctcctggtta tctggccaca ggcagcggta
    15961 acacccctgc tcccagggcg gcgtcagtac catcaactga ccgccaaatc cgaccgcgct
    16021 ggcggtgata agcggcgtgt tgagtaccac gcaggcggca ttaatctcct ggcgagtcgc
    16081 catattgtcg gtacagtcga gcaccacatc ggcccgtgca accgcatctt ttaacgcctc
    16141 acccgttaac cgttgttgta atgctgtcag ttgaatgtcg ggattcaact gtgtcagtcg
    16201 ctgttggctg acctgcgatt tcgggctgtt gatatcttca gtggtaaaga ggatttgtcg
    16261 ttgcagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc cgacgccagc
    16321 gcccgccagg tatagcgcag caggtgtacc cagcccgcca agaccgataa tcagcacctg
    16381 gctgtcgagc agtttttgct gcccgtcaag agcgatatcg tcgagcagga tttggcggct
    16441 ataacgcata aaatcacggt cattcatcgc caactcctgc aatttccagc aactgtgccg
    16501 ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgac gacggcgata ctgccgacac
    16561 ccgttgctat caccgcaggc gcgcgtgcca gactgatacc gccaatcgcc acggtgggat
    16621 aatccgccag tcgctcaaca tgccgtgcca gctgttccag cccctgcggt gcagaaggca
    16681 tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg cgtgctgcca
    16741 gcgcgacgtc gatttccata tcgtcatgtg tcgaaacacc cagccgcagg cctgccgcgc
    16801 ggatggcatt gagatcggtg gcttgcaaat cttcctgccc caaatggacg ccatacgcct
    16861 gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa tcgcgcgtta tagcggcggc
    16921 ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgccga tctttgatgc
    16981 gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt acgctgtcca
    17041 ccactgggta cagtcctaaa cgaaaaggta caggaggaaa atcaggctga tacatcacgc
    17101 ttcctcctta cgcaggtaga tttctccgcc tctggcacgg aagttctccg acatatccgc
    17161 cattcccatt tcaatagttt gcgtggcggc gtaatcacgc acttcctggc tgattttcat
    17221 cgagcagaat ttcggcccac acatggagca aaaatgggcg actttacctg actcttgcgg
    17281 cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat taaactggtc
    17341 ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt gcgcgcccgg
    17401 atgccctttc gccaggtcag cggcgtgggc ggcaatctta taggtgataa gcccctgctt
    17461 aacatcttct ttattaggca gacccagatg ctcttttggc gttacgtaac agagcatcgc
    17521 gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt catagcccgg
    17581 cgcaatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc agtgctctaa
    17641 ctcctcggtc atattgcggc ggatcatctg catcggcacg tggcctgggc cttcaatcat
    17701 cacctgcacg tcatattccc aggcaatttt ggtcagttcg cccagcgtat gcagctcggc
    17761 aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg cgcagaccgt cgcccagcga
    17821 cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt gatagaggaa
    17881 attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc gagagacgat
    17941 accggtcagg cgtttcgcgg tcatcggcac atagcgcagc agtacgcccg catggatagt
    18001 gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg cttcccaggt
    18061 aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca ctgtaccgat
    18121 cggcaccggg ctgttacgca aaatccactc gcgggtttcg tgaatatagc gaccggtgga
    18181 gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt ccacttcttc
    18241 ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta ccaggaaatt
    18301 gcgaccaata atcatcggct ccgattccgg atgattaatg ttggccggga taatcgcacg
    18361 tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca gatgtgcgcc
    18421 aaagctcatt cccggatgct ggtggcgtaa aacctcgcta cggatgcgct cgcggcccat
    18481 attctcgcgg atggcgatga attccatttc cggcgtgatg atgccctggc gggcgtagtg
    18541 cagttgggtg acacggcgtc ctgctttggc gcgttttggt gttagtacgc cgctaaaacg
    18601 cagttcgtcg aggccatcat ctgccagccg cgctttagtg taatcggaac tgcgcacggt
    18661 aagttcttcg gtatcgccgc gcgcatcgat ccacggctgg cgtagttttg ccagcccttg
    18721 ctgcacgtta atggcaatct gcggatcacc atacgggccg gaggtgtcgt agaccggaat
    18781 cgcttcgttt tcttcgtact gcggctgttc tttgctaccg ccaattagcg tcgggctaag
    18841 ctggatctca cgcatcggca cgcgcacgcc ggggtgtgtg ccagtgatat aaatgcgttt
    18901 tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggccc gggcgcgttg
    18961 ttcgcggcgg gtcagttttg ttgcagacat agctcattcc aaaaagttaa ggacgtggct
    19021 tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat agcagattaa
    19081 ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga ataaacggtc
    19141 tcagccagtt aaggcactcc gacaagaaat tagcccccac aaacggggcg ttgaatgtaa
    19201 attacgcgtt aacagcgcag aactcaagca ggatgtttga cgcgggcggc gtcaagcacc
    19261 agcagaatca gcttatcttc cagcacaaag cgcgcttcca gcgcctcgcc gatgtcagat
    19321 aaaacctgtt gaaactcaag gtaattatca tgatcgatgg cggtttccag gctggaatcg
    19381 tagtaatcca taatctgttg ggtattggct tcgagttgcg gccaaatctt cgcggcgcgc
    19441 gcgagttgcc cgttgccttc cagcttatga agaatgcgct cataaatact gaaatgtccg
    19501 gcagacaagt aatcgaccag gctctgacaa aaatcatcaa gggctttttc gtttagcctc
    19561 atgtacgatt ctttgccagg cttaatgcca accagattgt agtaagccac gagcagatgc
    19621 ttacgtacat gtagccagcg atcaaccagt ttgttacttc ctctgacgcg ctccgtcagg
    19681 ttatcgagct ggttaagcat gattgactcc gcaagtttgt attcaaaaac tgctcagtga
    19741 gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag gtagtgcaag agctatggat
    19801 cgtataattg aaaaattaga tcacggctgg tgggtcgtca gccatgaaca aaaattatgg
    19861 ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt tcgatcttgt gggtcagcgc
    19921 gcactacaaa tcggcgaatg gcagggggaa cctgtttggt tagtacaaca gcagcggcgt
    19981 cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg ttgggctgtt tcaactggcc
    20041 ggacgaggcg tacaactggc ggagttttac cgatcgcata aatactgtgg ttactgcggg
    20101 catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt gcagccattg ccgtgagcgt
    20161 tactacccgc aaatcgcccc ctgcattatt gttgccatcc gtcgcgatga ttcgatcctc
    20221 ctcgcccagc atacccgcca tcgtaacggt gtccatacag tacttgccgg attcgtcgaa
    20281 gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga tggaagagag cggaattaaa
    20341 gttaaaaact tgcgttacgt gacttctcag ccgtggccgt ttcctcagtc tttaatgacc
    20401 gcgtttatgg cggaatatga cagcggcgac atcgtgatcg acccgaaaga attgctcgag
    20461 gcgaactggt atcgctatga cgatttgccg ttactcccgc cgcccggcac cgtagcgcgc
    20521 cgtctgatag aagatactgt ggcgatgtgt cgggcagagt atgagtgatg atacactgac
    20581 cgcctgacgc actaaggaac agccaaaatg accgaactta aaaacgatcg ttatctgcgg
    20641 gcgctgctgc gccagcccgt tgatgtcact ccagtatgga tgatgcgcca ggcgggtcgc
    20701 tatctaccgg aatataaagc cacgcgcgcc caggcgggcg attttatgtc gctgtgcaaa
    20761 aacgccgagc tggcgtgcga agtgactttg caaccgctgc gtcgctaccc gctggatgcg
    20821 gcgatcctct tttccgatat cctcaccgtg ccggacgcga tggggttagg gctctatttt
    20881 gaagccggag aaggtccgcg ttttacctcg ccagtcacct gcaaagccga cgtcgataaa
    20941 ctgccaattc cggacccgga agatgagctg ggttacgtga tgaacgcggt gcgtaccatt
    21001 cgtcgcgaac tgaaaggcga agtgccgctg attggttttt ccggcagccc gtggacgctg
    21061 gcaacctaca tggtggaagg cggcagcagc aaagccttca ccgtgatcaa aaaaatgatg
    21121 tatgccgatc cgcaggcgct gcacgctctg ctcgataaac tggcgaaaag cgtcactttg
    21181 tatctgaatg cgcagattaa agccggtgct caggcagtga tgattttcga cacctggggc
    21241 ggcgtgctta ccgggcgcga ttatcaacag ttctcgctct attacatgca taaaattgtt
    21301 gatggtttac tgcgtgaaaa cgacggtcgc cgcgtaccgg tcacgctgtt taccaaaggc
    21361 ggcggacagt ggctggaagc catggcagaa accggttgcg atgcgctggg cctcgactgg
    21421 acaacggaca tcgccgatgc gcgccgccgt gtgggcaata aagtcgcgtt gcagggtaat
    21481 atggatccgt cgatgctgta cgcgccgcct gcccgcattg aagaagaagt agcgactata
    21541 cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc ttggtcacgg cattcatcag
    21601 gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag tgcatcgact gtctgaacag
    21661 tatcaccgct aagagcctgg tccatcaggc tattttattt gccattttga ggccgggcag
    21721 tgctcaaaat cctcacgtac tacgtgtacg ctccggtttt tccgcgctgt ccggcctcaa
    21781 actgccttca acaattacgc ctgttgaacc aggctctaag gagcgattat ggatctcgcg
    21841 tcattacgcg ctcaacaaat cgaactggct tcttctgtga tccgcgagga tcgactcgat
    21901 aaagatccac cggatctgat cgccggagcc gatgtcgggt ttgagcaggg cggagaagtg
    21961 acgcgagcgg cgatggtgct gctgaaatat ccctcgcttg agctggtgga gtataaagtt
    22021 gcccgcatcg ccaccaccat gccttacatt ccaggttttc tttccttccg cgaatatcct
    22081 gcgctgctgg cagcgtggga gatgctgtcg caaaagccgg atttagtgtt tgtcgatggt
    22141 catgggatct cgcatcctcg ccgtcttggc gtcgccagcc attttggttt gatggtggat
    22201 gtgccaacga tcggcgtggc gaaaaaacgg ctctgcggta aattcgaacc gctctccagc
    22261 gaaccgggcg cgctggcccc actgatggat aaaggcgagc agctggcctg ggtctggcgc
    22321 agcaaagcgc gctgtaaccc gttgtttatc gctaccggcc atcgggtcag cgtggacagc
    22381 gcgctggcgt gggtacaacg ctgcatgaaa ggctatcgtc tgccggagcc aacgcgctgg
    22441 gcggacgcgg tggcctcgga acgtccggcg ttcgtgcgct atacagcaaa tcagccctaa
    22501 ttcaggtaaa ctgcggataa tttccgtatt tgagaactca acatgttaca aaacccaatt
    22561 catctgcgtc tggagcgcct ggaaagctgg cagcatgtca ctttcatggc ttgcttatgc
    22621 gaacgcatgt accccaatta cgccatgttc tgccagcaaa ccggttttgg tgatgggcaa
    22681 atttaccgtc gtattctcga tctcatctgg gaaacgctga ccgttaaaga cgcaaaagta
    22741 aatttcgaca gccaactgga gaaatttgaa gaagcgattc cttcagccga cgatttcgat
    22801 ctgtacggcg tttatccggc aatcgatgcc tgcgtggcgt taagtgaact ggtccattcg
    22861 cgtttgagtg gtgaaacgct cgaacacgcg gtggaagtga gtaagacctc catcacgacc
    22921 gttgcgatgc tggaaatgac tcaggctggt cgcgaaatga gcgatgaaga gcttaaagaa
    22981 aacccagctg tagagcaaga atgggacatt cagtgggaaa tattccgact tttagctgag
    23041 tgcgaagaac gcgatatcga gctgataaaa ggccttaggg cagacctgcg tgaggcgggt
    23101 gagagcaata ttggtataat ttttcagcaa taagaccaga aaacgtgatt taacgcctga
    23161 tttgtcgtac ctggagtctt ccctttcgcc ccccgtctgg tctacatttg gggggcgaaa
    23221 aaaagtggct atcggtgcgt gtatgcagga gagtgctatt ctggcatttc cgtcgcactc
    23281 gatgcttagc aagcgataaa cacattgtaa ggataactta tgaacaagac tcaactgatt
    23341 gatgtaattg cagagaaagc agaactgtcc aaaacccagg ctaaagctgc tctggagtcc
    23401 actctggctg caattactga gtctctgaaa gaaggcgatg ctgtacaact ggttggtttc
    23461 ggtaccttca aagtgaacca ccgcgctgag cgtactggcc gcaacccgca gaccggtaaa
    23521 gaaatcaaaa ttgccgcagc taacgtaccg gcatttgttt ctggcaaggc actgaaagac
    23581 gcagttaagt aagattgcgt ggcagtgaac agttttaacg aaggggtggt ttcacccctt
    23641 ttgtctttct ggcgtcgatc attgatgctg gctggcgctc tgcttctcac tgcctgtagt
    23701 cataactctt cacttcctcc ctttaccgcc agtggatttg ctgaagacca gggcgtggta
    23761 cgcatctggc gaaaagacag cggcgataat gtgcatctgc ttgccgtgtt tagcccgtgg
    23821 cgcagtggcg ataccacgac gcgagagtat cgctggcagg gcgataacct cacgctcatc
    23881 aatatcaatg tttacagcaa accgccggtg aatattcgcg cgcgttttga cgatcgcggt
    23941 gatctgagct ttatgcaacg tgaatccgat ggggaaaagc agcagctttc taacgaccaa
    24001 atcgatttat accgttatcg tgctgatcag atccgccaga ttagcgatgc cttacgtcag
    24061 gggagagtcg tgctgcgcca ggggcgctgg catgcgatgg aacagaccgt gaccacctgc
    24121 gaagggcaaa ccattaaacc tgatttagat tcgcaggcga tagcgcatat cgagcgccgc
    24181 cagagccgct cttctgttga tgtcagcgtg gcatggctgg aagcgcccga aggttcgcaa
    24241 ttactgttag tggcaaactc tgatttctgt cgctggcaac ccaacgagaa aacgttctga
    24301 tttaccagtg gcccataccc atatgaccgc caccaccgca gccgccgtag cccatgccca
    24361 ttccggctcc gcgcggaata cccgcttcag ccatcgcgat atctcgtttc acccgtaact
    24421 catctaacga ctgacgcaaa ttctccatct ctttggcgac cgcgttaatt ttgctgctat
    24481 ccggtgggtt cgcggctaac agggcattgt attcataacg cttcgtcacc agttgctgtt
    24541 gcagtgcgct gctttgagcg taaaagtcat tatggatttt ctgccacgct gtctgttgtt
    24601 cgctggtcaa aggcgcggca ttttgctgcc acataccgtg tccgccgtga gcaaatgcag
    24661 atgtcgatcc catcgccatt gctgaaagca ccatcattac cagggcaatt ttcgtgttcc
    24721 gtttcatggt taatcctcca gtggttgtct tacttcgagt attgcatctt ccgtgccaac
    24781 gatgaaacgc tgatatgacg ggtaatctgg catgataaac gagtaaaaat gactcgcctg
    24841 ctgcgggtag cgagtcattt ttactcattg aaacttaagc ctttgtgtta cagcgcaggg
    24901 taagcgctga taaaagatgg catgatttct gctgtcagaa agggatgagc aggcaaagaa
    24961 gaagatgcgt tttatgcaac gttctaaaga ctccttagct aaatggttaa gcgcgatcct
    25021 ccccgtggtc attgttgggc tggtagggtt gtttgcggtg acggtgattc gcgattatgg
    25081 gcgcgagact gccgccgcca gacaaacgct gctggaaaaa ggcagtgtac ttattcgcgc
    25141 tcttgaatcc ggctcgcgcg tcggcatggg gatgcgcatg catcatgcgc agcagcaggc
    25201 cttactggaa gaaatggccg ggcagcctgg agtacgttgg tttgcggtca cggatgaaca
    25261 aggaacaatc gtgatgcata gcaactccgg catggtggga aaacagcttt attcccctca
    25321 ggaaatgcag cagttacatc cgggagatga agaagcgtgg cggcggatcg atagcgcaga
    25381 cggcgagcct gttctggaaa tttatcgcca gtttcaaccg atgtttgctg ctggaatgca
    25441 ccggatgcgc catatgcagc agtatgccgc gacaccacaa gcaattttca tcgctttcga
    25501 cgccagtaat attgtgagtg ccgaagatcg tgagcagaga aacaccctga ttattctctt
    25561 cgccctggcg acggtcttgc tggcaagcgt attgtcattc ttctggtatc gccgctatct
    25621 gcgctcgcgc cagcttctac aagatgaaat gaagcgcaaa gagaagctgg tggcgctggg
    25681 gcatcttgcg gcaggcgttg cccacgaaat ccgtaaccca ctttcctcga ttaaaggact
    25741 ggcgaaatac tttgccgagc gcgcgcctgc agggggagaa gcgcatcaac tggcgcaggt
    25801 gatggcgaaa gaggccgacc gtttaaaccg cgtggtaagc gagttgctgg aactggttaa
    25861 gccaacgcat ctggctttgc aggcggtgga tctcaacacg ctgattaacc actcattaca
    25921 gctggtaagc caggatgcaa acagccggga gatccagtta cgctttaccg ccaacgacac
    25981 attaccggaa attcaggccg atccggacag gctgactcag gtcctgttga atctctatct
    26041 caatgctatt caggcgattg gtcagcatgg cgtgattagc gtgacggtca gcgaaagcgg
    26101 cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga attgcggcag atcagcttga
    26161 agccatcttc actccgtact tcaccaccaa agccgaaggc accggattgg ggctggcggt
    26221 cgtgcataat attgttgaac aacacggtgg tacaattcag gtcgcaagcc aggagggaaa
    26281 aggctcaacg ttcaccctct ggcttccggt caatattacg cgtaaggacc cacaaggatg
    26341 acgcacgata atatcgatat tctggtggtg gatgatgaca ttagccactg cactattttg
    26401 caggctttac tgcgcggctg gggctataac gtcgcgctgg cgaacagcgg gcgacaggcg
    26461 ctggagcagg tgcgggaaca ggtttttgat cttgtgcttt gcgatgtgcg aatggcggag
    26521 atggacggca tcgccacgct gaaagagatc aaagcgttaa acccggcaat tccggtgctg
    26581 attatgactg cgtactccag cgtcgagacg gcggtagagg cactgaaaac tggggcgctg
    26641 gattatctca tcaagccgct ggatttcgat aacctacagg cgacgctgga aaaagcgctc
    26701 gcgcatacgc acagtattga tgctgaaaca cctgcggtga ctgccagcca gttcggtatg
    26761 gtcggtaaaa gcccggcgat gcaacacctg ctcagtgaaa tcgccctcgt cgcgccatcg
    26821 gaagccacgg tactgatcca cggcgattcc ggcaccggta aagagctggt cgccagggcg
    26881 attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa cgctcaactg tgcggcactc
    26941 aacgaatcct tactggaatc tgaattgttc ggtcacgaaa aaggggcgtt tactggagcc
    27001 gataaacgcc gggaagggcg ctttgttgag gcggacggcg gcacgctgtt tctcgatgaa
    27061 attggcgata tctcgccgat gatgcaggtg cgtctgctgc gtgcgattca ggagcgcgaa
    27121 gttcagcgtg tcggtagcaa ccagactatc tcggttgatg tccggctgat tgcggcgacc
    27181 catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc gccaggatct ctactatcgc
    27241 ctgaatgtgg tggcgattga agtaccatcg ctgcggcaac ggcgggaaga tatccctctg
    27301 ctggctggcc attttctgca gcgctttgcc gagcgtaatc gcaaggcggt aaaaggtttt
    27361 acgccccagg cgatggatct gttgattcat tacgactggc cgggaaatat tcgtgagctg
    27421 gaaaacgcgg tggaacgggc agtggtgctg ctgaccgggg aatatatttc cgaacgcgag
    27481 ctgccgctgg cgattgccag tacgccgatc ccgctggggc aaagtcagga tattcagccg
    27541 ctggtggaag tggaaaaaga ggtgattctg gcggcactgg agaaaacggg cggcaacaaa
    27601 actgaagccg cccgtcagtt agggatcacg cgtaaaacgt tattggcaaa actgtcgcgt
    27661 tagttctgct cgcgttcgat agcacgccag ccgatatctt tccggcagaa gcagtcgtcc
    27721 cagtggatat cggtcattaa ggcataggcg cgtttctgcg cttctgccac ggtatgaccc
    27781 agcgcggtga cgcacagtac gcgtccgccg ttggtcacta cctgctcgtc atccgccagt
    27841 tttgtgcccg cgtggaacac tttgccgcct gccacttctt ccagcggcag gccgtggatc
    27901 acgtcaccgg tgcggtaatc acccggatat ccacccgcag ccatcaccac gccgagagaa
    27961 gcgcgttcat tccactcgga cgttttctcg tccagtttgc tttcacaggc cgccaggcag
    28021 agctcaacca gatcggactt catgcgcagc ataatcggct gggtttccgg atcgccaaag
    28081 cggcagttaa attcaataac cttcggattg ccctgtttgt cgatcatcag gcccgcgtag
    28141 agaaaaccgg tgtaggtgtt gccttccgcc gccatgcctt tcacggttgg ccagataata
    28201 cgttccatgg tgcgctgatg aacgtcatcg gttactaccg gcgcggggga gtaagcgccc
    28261 atcccgccgg tgtttggtcc ggtatctttg tcgcctacgc gtttgtgatc ctggctggta
    28321 gccatcggca gcacatgctc gccgtccacc atcacgataa agctcgcttc ttcgccatcg
    28381 aggaactctt cgataacgat gcgatgaccc gcgtcgccaa aagcgttgcc cgccagcata
    28441 tcgtgaacag ccgcttccgc ttcttccagc gtcatcgcca cgataacgcc tttcccggca
    28501 gccagaccgt ccgctttaat gacgattggc gcgcctttct cacgcagata cgccagcgca
    28561 ggttctacct cggtgaagtt ctggtattcc gccgtaggga tcttatggcg ggccaggaaa
    28621 tctttggtaa acgcttttga gccttccagt tgggccgcac ctgcggttgg gccgaagatt
    28681 ttcagcccgg cggcgcggaa ggtatcgacc acgcctttca ccagaggcgc ttccgggccg
    28741 acgatggtca gatcaatctt ttcgttttgt gcgaaatcca acagcgccgg gatatcggtc
    28801 acgccaatag caacgttttg cagcgcgggt tccagcgcag tgcctgcatt acccggagca
    28861 acaaaaacag tctcaaccag cggcgactgg gccgctttcc aggccagcgc gtgctcgcgc
    28921 ccgccgttac caatcactaa tactttcatc tattgctcca ttaatggcgg aagtggcgca
    28981 tgtcggtgaa gagcatcgca ataccgtgct cgtcggcggc ggcaatcact tcgtcatcac
    29041 ggatggaacc gccaggctgg attacgcagg tcacgcccgc agcggcggcg gcatcaatac
    29101 cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga acctttcact tccaggcctt
    29161 catcggcagc tttaataccg gcgattttcg cggagtacac gcggctcatc tggcccgcgc
    29221 caataccgat ggtcatattg tttttggcat agacgatagc attggatttc acgaacttcg
    29281 ccactttcca gcagaacagc gcatcgcgca gttcctgttc ggtcggctga cgttgggtga
    29341 cgacgcgcag ttcttccgca ccgaccatcc ccaggtcacg atcctgaacc agcagaccgc
    29401 cgttcacgcg tttgaaatca agacccggaa cacgctcgcc ccactgaccg caggtcagaa
    29461 cgcgtacgtt ctgcttggcg gcggtgattt tcagggcttc ttcgctggcg gaaggcgcaa
    29521 taatcacttc gacaaactga cgagaaatga tggcctgtgc ggtttccgca tctagctcgc
    29581 ggttaaaggc aatgatgccg ccgaatgcgg aggttgggtc ggttttgtac gcgcgatcgt
    29641 aagcatcaag aatggaattg ccgatagcta caccgcaagg gttggcgtgc ttcacaatca
    29701 cacatgccgg ctcggcgaac tctttcacgc actccagcgc cgcatcggta tcggcgatgt
    29761 tgttataaga gagggcttta ccctgaacct gggttgcggt agcaacggag gcttctttca
    29821 cattctcttc tatatagaag gcagcctgct ggtggctgtt ctcgccgtaa cgcatatcct
    29881 gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg accggcggct tctttgcttt
    29941 caccgtggta cgccggaacc atgctgccga agtagttggc aatcatgctg tcgtaggcgg
    30001 cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt tgcaagcgtc agcgatcctt
    30061 cgttgtcatc catctcttta ataatggcgt cgtagtcgct gctcttcacc acgattgcga
    30121 catctttatg gttcttagcg gcggagcgca ccatggtcgg gccgccgata tcgatgttct
    30181 caaccgcatc ttccagcgag cagccttcac gagcaacagt ctgggcgaac ggatacaggt
    30241 taacaaccac catatcgata ggctggatct gatgttcttc cataatggca tcgtcctggc
    30301 cgcgacggcc cagaatgcca ccatgtactt tcggatgcag ggtcttcacg cgtccatcca
    30361 tcatctccgg gaaaccggtg taatcggaaa cttcggttac cggcagacct ttttctgcta
    30421 acagacgggc agtgccccct gtagacagta gctccacacc gcgtgcggaa agtgcctggg
    30481 cgaattcgac gataccggct ttgtcagaaa cactgagcag agcgcggcgg actggacgac
    30541 gttgttgcat ggtaaatccc ctggatttga ctattacaga gagcgttagc tgaatttttc
    30601 gcgaaaaact cagctaacgc ccctaacggg gcatccttat ttttcgcccg cattgtaacg
    30661 aaaacgtttg cgcaacgctc gcgaattttt ctctttcaat ggtgatcaca attttgacag
    30721 tggttaccgt gggaaaaata cagaaattac attgatgatt gtggataact ctgtgcgtaa
    30781 aaaggtataa agcggggttt tgctggggaa tgcagcagtc agtcattttt ctgcaatttt
    30841 tctattgcgg cctgcggaga actccctata atgcgcctcc atcgacacgg cggatgtgaa
    30901 tcacttcaca caaacagccg gttcggttga aaagaaaaat cctgaaattc agggttgact
    30961 ctgaaagagg aaagcgtaat atacgccacc tcgcgacagt gcgctaaag
//
LOCUS       DAZKHC020000051        29838 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZKHC020000051 DAZKHC020000000
VERSION     DAZKHC020000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.051"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(21..1850)
                     /gene="glmS"
                     /locus_tag="ACHOSM_RS13325"
                     /old_locus_tag="pgaptmp_002665"
     CDS             complement(21..1850)
                     /gene="glmS"
                     /locus_tag="ACHOSM_RS13325"
                     /old_locus_tag="pgaptmp_002665"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGU4559160.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2012..3382)
                     /gene="glmU"
                     /locus_tag="ACHOSM_RS13330"
                     /old_locus_tag="pgaptmp_002666"
     CDS             complement(2012..3382)
                     /gene="glmU"
                     /locus_tag="ACHOSM_RS13330"
                     /old_locus_tag="pgaptmp_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGU4559161.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGCEIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3733..4152)
                     /gene="atpC"
                     /locus_tag="ACHOSM_RS13335"
                     /old_locus_tag="pgaptmp_002667"
     CDS             complement(3733..4152)
                     /gene="atpC"
                     /locus_tag="ACHOSM_RS13335"
                     /old_locus_tag="pgaptmp_002667"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGU4559162.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4173..5555)
                     /gene="atpD"
                     /locus_tag="ACHOSM_RS13340"
                     /old_locus_tag="pgaptmp_002668"
     CDS             complement(4173..5555)
                     /gene="atpD"
                     /locus_tag="ACHOSM_RS13340"
                     /old_locus_tag="pgaptmp_002668"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGU4559163.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5582..6445)
                     /gene="atpG"
                     /locus_tag="ACHOSM_RS13345"
                     /old_locus_tag="pgaptmp_002669"
     CDS             complement(5582..6445)
                     /gene="atpG"
                     /locus_tag="ACHOSM_RS13345"
                     /old_locus_tag="pgaptmp_002669"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGU4559164.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6496..8037)
                     /gene="atpA"
                     /locus_tag="ACHOSM_RS13350"
                     /old_locus_tag="pgaptmp_002670"
     CDS             complement(6496..8037)
                     /gene="atpA"
                     /locus_tag="ACHOSM_RS13350"
                     /old_locus_tag="pgaptmp_002670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGU4559165.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8050..8583)
                     /gene="atpH"
                     /locus_tag="ACHOSM_RS13355"
                     /old_locus_tag="pgaptmp_002671"
     CDS             complement(8050..8583)
                     /gene="atpH"
                     /locus_tag="ACHOSM_RS13355"
                     /old_locus_tag="pgaptmp_002671"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGU4559166.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8598..9068)
                     /gene="atpF"
                     /locus_tag="ACHOSM_RS13360"
                     /old_locus_tag="pgaptmp_002672"
     CDS             complement(8598..9068)
                     /gene="atpF"
                     /locus_tag="ACHOSM_RS13360"
                     /old_locus_tag="pgaptmp_002672"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGU4559167.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9130..9369)
                     /gene="atpE"
                     /locus_tag="ACHOSM_RS13365"
                     /old_locus_tag="pgaptmp_002673"
     CDS             complement(9130..9369)
                     /gene="atpE"
                     /locus_tag="ACHOSM_RS13365"
                     /old_locus_tag="pgaptmp_002673"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGU4559168.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9416..10231)
                     /gene="atpB"
                     /locus_tag="ACHOSM_RS13370"
                     /old_locus_tag="pgaptmp_002674"
     CDS             complement(9416..10231)
                     /gene="atpB"
                     /locus_tag="ACHOSM_RS13370"
                     /old_locus_tag="pgaptmp_002674"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGU4559169.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10240..10620)
                     /gene="atpI"
                     /locus_tag="ACHOSM_RS13375"
                     /old_locus_tag="pgaptmp_002675"
     CDS             complement(10240..10620)
                     /gene="atpI"
                     /locus_tag="ACHOSM_RS13375"
                     /old_locus_tag="pgaptmp_002675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HGU4559170.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11237..11860)
                     /gene="rsmG"
                     /locus_tag="ACHOSM_RS13380"
                     /old_locus_tag="pgaptmp_002676"
     CDS             complement(11237..11860)
                     /gene="rsmG"
                     /locus_tag="ACHOSM_RS13380"
                     /old_locus_tag="pgaptmp_002676"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGU4559171.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11924..13813)
                     /gene="mnmG"
                     /locus_tag="ACHOSM_RS13385"
                     /old_locus_tag="pgaptmp_002677"
     CDS             complement(11924..13813)
                     /gene="mnmG"
                     /locus_tag="ACHOSM_RS13385"
                     /old_locus_tag="pgaptmp_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGU4559172.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14192..14635)
                     /gene="mioC"
                     /locus_tag="ACHOSM_RS13390"
                     /old_locus_tag="pgaptmp_002678"
     CDS             complement(14192..14635)
                     /gene="mioC"
                     /locus_tag="ACHOSM_RS13390"
                     /old_locus_tag="pgaptmp_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HGU4559173.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14725..15183)
                     /gene="asnC"
                     /locus_tag="ACHOSM_RS13395"
                     /old_locus_tag="pgaptmp_002679"
     CDS             complement(14725..15183)
                     /gene="asnC"
                     /locus_tag="ACHOSM_RS13395"
                     /old_locus_tag="pgaptmp_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HGU4559174.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15335..16327
                     /gene="asnA"
                     /locus_tag="ACHOSM_RS13400"
                     /old_locus_tag="pgaptmp_002680"
     CDS             15335..16327
                     /gene="asnA"
                     /locus_tag="ACHOSM_RS13400"
                     /old_locus_tag="pgaptmp_002680"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGU4559175.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16332..17783)
                     /gene="viaA"
                     /locus_tag="ACHOSM_RS13405"
                     /old_locus_tag="pgaptmp_002681"
     CDS             complement(16332..17783)
                     /gene="viaA"
                     /locus_tag="ACHOSM_RS13405"
                     /old_locus_tag="pgaptmp_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HGU4559176.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIMQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGHLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSHLLRRWRR"
     gene            complement(17777..19273)
                     /gene="ravA"
                     /locus_tag="ACHOSM_RS13410"
                     /old_locus_tag="pgaptmp_002682"
     CDS             complement(17777..19273)
                     /gene="ravA"
                     /locus_tag="ACHOSM_RS13410"
                     /old_locus_tag="pgaptmp_002682"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HGU4559177.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSSRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19496..21364
                     /gene="kup"
                     /locus_tag="ACHOSM_RS13415"
                     /old_locus_tag="pgaptmp_002683"
     CDS             19496..21364
                     /gene="kup"
                     /locus_tag="ACHOSM_RS13415"
                     /old_locus_tag="pgaptmp_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HGU4559178.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWSLIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21531..21950
                     /gene="rbsD"
                     /locus_tag="ACHOSM_RS13420"
                     /old_locus_tag="pgaptmp_002684"
     CDS             21531..21950
                     /gene="rbsD"
                     /locus_tag="ACHOSM_RS13420"
                     /old_locus_tag="pgaptmp_002684"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HGU4559179.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            21958..23463
                     /gene="rbsA"
                     /locus_tag="ACHOSM_RS13425"
                     /old_locus_tag="pgaptmp_002685"
     CDS             21958..23463
                     /gene="rbsA"
                     /locus_tag="ACHOSM_RS13425"
                     /old_locus_tag="pgaptmp_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HGU4559180.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23468..24433
                     /gene="rbsC"
                     /locus_tag="ACHOSM_RS13430"
                     /old_locus_tag="pgaptmp_002686"
     CDS             23468..24433
                     /gene="rbsC"
                     /locus_tag="ACHOSM_RS13430"
                     /old_locus_tag="pgaptmp_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGU4559181.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24458..25348
                     /gene="rbsB"
                     /locus_tag="ACHOSM_RS13435"
                     /old_locus_tag="pgaptmp_002687"
     CDS             24458..25348
                     /gene="rbsB"
                     /locus_tag="ACHOSM_RS13435"
                     /old_locus_tag="pgaptmp_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HGU4559182.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25474..26403
                     /gene="rbsK"
                     /locus_tag="ACHOSM_RS13440"
                     /old_locus_tag="pgaptmp_002688"
     CDS             25474..26403
                     /gene="rbsK"
                     /locus_tag="ACHOSM_RS13440"
                     /old_locus_tag="pgaptmp_002688"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGU4559183.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26407..27399
                     /gene="rbsR"
                     /locus_tag="ACHOSM_RS13445"
                     /old_locus_tag="pgaptmp_002689"
     CDS             26407..27399
                     /gene="rbsR"
                     /locus_tag="ACHOSM_RS13445"
                     /old_locus_tag="pgaptmp_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HGU4559184.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27365..28792)
                     /gene="mdtD"
                     /locus_tag="ACHOSM_RS13450"
                     /old_locus_tag="pgaptmp_002690"
     CDS             complement(27365..28792)
                     /gene="mdtD"
                     /locus_tag="ACHOSM_RS13450"
                     /old_locus_tag="pgaptmp_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HGU4559185.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPISGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(28815..29507)
                     /gene="yieP"
                     /locus_tag="ACHOSM_RS13455"
                     /old_locus_tag="pgaptmp_002691"
     CDS             complement(28815..29507)
                     /gene="yieP"
                     /locus_tag="ACHOSM_RS13455"
                     /old_locus_tag="pgaptmp_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HGU4559186.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7182 a         7653 c         7625 g         7378 t
ORIGIN      
        1 ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt acgcggctgg
       61 tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag cggaacggtg
      121 tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg catgttatcg
      181 ctacttacaa aacccgcatc ctgatcggcg aagacataca actgaccgcc acgcgcgcga
      241 acttcttcaa tgttggattt cagtttttcc aacagttcgt tgttcggtgc aacgacgata
      301 accggcatat ccgcgtcaat cagcgccagc gggccgtgtt tcagctcgcc tgcagcgtag
      361 gcttcagcgt gaatgtaaga gatctctttc agcttcaatg cgccttccag cgcgattggg
      421 tactgatcgc cacggcccag gaacagcgcg tgatgtttgt cagagaaatc ttctgccaga
      481 gcttcaatgc gtttgtcctg agacagcatc tgctcaatac ggctcggcag cgcctgcaga
      541 ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga cagcttcgcc
      601 accagcatca acagcacagt taactgagtg gtgaatgctt tagtggatgc cacgccgatt
      661 tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga agaacccgga
      721 acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg caggcccgcc
      781 agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt acgacgcacg
      841 gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat acctgccagc
      901 gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca ggcgaggatc
      961 tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc gcttaaatca
     1021 acctgaccgt ggctgatgcg cccggtaagg gtgtttttta tcgcgttcgg ctgttcgtag
     1081 atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata ttgcagattg
     1141 gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt taccgaacgg
     1201 cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt caccggcaac
     1261 agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaggccaat caccagcgga
     1321 ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat cactgtaccg
     1381 tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt cccgccttgt
     1441 ttcagctccc agttcaccag atgggcaatt acttcggtgt ctgtttcaga aacgaaggta
     1501 tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat gccgttatgc
     1561 accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg ttcaccgtgg
     1621 gtcgcccagc gagtgtgagc aataccggtg ccgccatgca gaggatgttc ttccgctgcc
     1681 tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc ttccgcatca
     1741 acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa accttcaaga
     1801 aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat agtttttgat
     1861 tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc cccgagcctt
     1921 gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat gttatctcct
     1981 catcccagat gaccggatta cccggccaga attacttttt ctttaccgga cgacgccagc
     2041 cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg acattacgcg
     2101 tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg gccaccagct
     2161 gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta ttcgcaccat
     2221 cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt tccgcatcgc
     2281 ccaggtaagt cagatgcccg gctttcgagc ctttacccag acgcgctttt ttcatctcaa
     2341 cgaagttacc aacgtgtgca ccttccagca actcagcacc aggacgcaga cgggcaaacg
     2401 ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac ggactgattt
     2461 cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt ttcacgcgat
     2521 gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca tcgcgcccgt
     2581 gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg cctgctaaca
     2641 gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt tgcaggcggt
     2701 tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt tcacaccctt
     2761 cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca ttattgttgg
     2821 tcagcttcgc cagccagcgt ttcatatctg cgccgttagc gatcagaatg ccggtgttga
     2881 tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg ccggtaactt
     2941 tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc accgtcagca
     3001 gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg acagagatca
     3061 gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa ggtgcggcct
     3121 gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta aggttgtcgt
     3181 ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaggtga acgtgcgctg
     3241 cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg gcaagggtat
     3301 gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca aggatcacta
     3361 cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga atttaaaccg
     3421 cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat aaattgttca
     3481 ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg ccttttgtct
     3541 gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg ctcttatttc
     3601 gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta tgtgaacgct
     3661 cttcaggacg gatcacgcgt aaaaaaaagc cagcctgttt ccagactggc ttttgtgctt
     3721 ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct gcgcgatcgc
     3781 tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc tgctaatgtg
     3841 ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct ggccgcgaat
     3901 tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc cgccagacag
     3961 atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag gcttaatggc
     4021 ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac ccgttacctg
     4081 gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt ccaggtggta
     4141 agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt ttccacagct
     4201 tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta ttcgccttcc
     4261 atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc cggagaaccg
     4321 gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt acgagcacgc
     4381 gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaggatggc gatgatgtct
     4441 ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc gtagtgttcc
     4501 tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc aacggccggg
     4561 tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag gtgcgcaaag
     4621 gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc ctgtacggag
     4681 gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat ctcttccgcc
     4741 agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga tacttccgta
     4801 ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg accttcgtca
     4861 cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt tcccggcggc
     4921 tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc ggtcatttcg
     4981 tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac agagtaaccg
     5041 gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt acctacaccc
     5101 gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag gtcgataact
     5161 ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga aggtgctgcg
     5221 cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac cggttcaccc
     5281 agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat cgggtgttcg
     5341 aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc gatggtacgc
     5401 acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt accattttgc
     5461 acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac gtcaactacg
     5521 gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg aaataacctg
     5581 tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg ctggcctgac
     5641 gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg tcggtcgcgg
     5701 ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc acgccctgat
     5761 aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc ggatcgggtt
     5821 cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc ggtaacggca
     5881 gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg ctgacaatgt
     5941 aaagcttgtc cagacggcct tcgtcgtagg cctgcaacat cacttttacc ggaccgatca
     6001 gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca ttgccgccca
     6061 cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat tgaacgcctt
     6121 tgtcggtcca ggtcttcatt tccgccagca gttttttgaa caggttaatg ttcaaaccac
     6181 cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg tcgcggtctt
     6241 ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc actttgcgca
     6301 tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc attttggaag
     6361 cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt gcgatcttac
     6421 tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc ctgccctaag
     6481 gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga tgcctttcag
     6541 cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca tcaacggagc
     6601 gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt tcgacagttc
     6661 aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct gcgcaacgga
     6721 catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt ggtcaagctg
     6781 cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca gttcacgata
     6841 ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg tctgtgctgc
     6901 accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac cggcgttgaa
     6961 caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg tcggaacgaa
     7021 cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag aaccggtttt
     7081 ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac gtgcagcacg
     7141 ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac gtcctggcgg
     7201 acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca ggtcatcgta
     7261 aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc aaccggcata
     7321 cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa cgatggtgtt
     7381 agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg acgctttctg
     7441 gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga tggcatcgat
     7501 agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct gaccacgacc
     7561 gattgggatc atggagtcaa cggctttata accggtctgt accggctgat ctacggactg
     7621 acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt gatccagcgg
     7681 acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca ggccacggcc
     7741 aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg caaggtcagc
     7801 gtacggaccc ataacaaccg cacctacaga gtcgcgctcg aggttcagtg cgatagcgta
     7861 acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggctaggc cgtgaatgcg
     7921 gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac tcacaacatt
     7981 gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca gttgcatgct
     8041 ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc gtacgctgcc
     8101 atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact tatcgatttt
     8161 gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa ttttcgcgag
     8221 ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag cctcactcac
     8281 ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac gaccattttc
     8341 agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac aaactgcgat
     8401 aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg ccatttgttc
     8461 gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta cactttggtg
     8521 ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa taaattcaga
     8581 catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc gctgttagca
     8641 gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag gatagcaact
     8701 tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc ctgcgcctgg
     8761 gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag aatctgcgag
     8821 cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt tttcagctgg
     8881 tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc ggaagcaagg
     8941 ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg ccatacgtac
     9001 ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat tgttgcgtta
     9061 agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt taaataaaag
     9121 caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag cgatcatcgg
     9181 gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag gaatcagatc
     9241 aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac cgatcgcagc
     9301 accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat ccatattcag
     9361 gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg gtaaattaat
     9421 gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg aaggcttgca
     9481 gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac tgtgaccacc
     9541 acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg ttaccgaaca
     9601 gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg attaagttga
     9661 caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg aagccgccga
     9721 tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccaat gccatagaca
     9781 gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc agtacatgtt
     9841 cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc aggaataccc
     9901 agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg tacatgtctt
     9961 tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac ttacctggca
    10021 caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac agacccagca
    10081 ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg gggttttgtg
    10141 gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg tgtcctatgt
    10201 aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt tgttaattac
    10261 agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca gcggcaagaa
    10321 taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca gaactttgaa
    10381 agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat gcgcctggtg
    10441 acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc ccccgcttat
    10501 tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg ctatcaccac
    10561 cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg acacagacat
    10621 cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa ctttctttaa
    10681 ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg tgatttcaaa
    10741 caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat acaattgcga
    10801 cttttctgct aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa cactggtgat
    10861 aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agcatattta tttacttggc
    10921 aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg ttttaataaa
    10981 tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta acagccaatg
    11041 atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt attttctgct
    11101 taccaaaaaa agccacgtta tcttgttgat gcaaaagagt gaacgtggcg ttaaatgtaa
    11161 ccagttatat cagtagaaaa tctggttgtt gttaacagtc taaccggtca attttttatg
    11221 atttttttga taaaaattaa attttatttg ctttaatcac caccagatga cgttcgccat
    11281 ccagggctgg aacctgaagt ttaaccactg attcgacctg atattcttcg ggcaacaaag
    11341 cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc tcaccaggaa
    11401 gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta attacgccat
    11461 caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggttca atattctcca
    11521 gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg ctatccaaca
    11581 gagtgaaatg ggcttcagga cgcacgatag agagtggaat gccaggcagt ccaggaccgg
    11641 tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg ctatcgagaa
    11701 tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac gctttgttcc
    11761 atttatgcag catattcacg taggcaataa gctggttttt ctggtgatcg gtaagcgaaa
    11821 tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc tgttcttgat
    11881 gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg ctacgacgca
    11941 gcataccctg ttttttcagc cacaccagca gaatggagat ggccgcaggc gtgacgccag
    12001 aaatacgtga agcctggccg atagaggccg gtttgtgatc gttaagtttg gcgatcactt
    12061 cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg gtgttctcgt
    12121 tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg tatttaacct
    12181 gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac ggcgtcagcg
    12241 tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca ctggcttcac
    12301 gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc gacggggtta
    12361 cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc tcgttaaagc
    12421 gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc aaacgcagat
    12481 ccgcattatc ttcgcgtagc atcagacgat attctgcgcg cgaagtaaac atacgatacg
    12541 gttctttggt tcctaaagtg cacaggtcat caactagtac gccgagatac gcctgagaac
    12601 gtgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga ccggccagca
    12661 aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca gcaaagaaca
    12721 gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgaggatcg aagaagtcat
    12781 actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc atagagcgga
    12841 cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga taaatttcat
    12901 tagaggtcag tccttccggt tcaaggaaga tctgatgctg atttctgtcg gcgaagcgca
    12961 tgactttgtc ttcgatcgac gggcagtagc gtgggccgac accttcgatc acccctgcat
    13021 acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg ttggtatgag
    13081 tgatataaca cggcacctgc tggggatgct gggacgcatt gcccataaac gagaataccg
    13141 gcattgggtt atcgccatgc tgttgcgcca gcacactaaa gtcgatagtt cgagcatcaa
    13201 tacgcggtgg tgtcccggtt ttcagacgac caacgcgcag cggcagttca cgcaaacggc
    13261 gagaaagcgg aatggacggc ggatcaccag cacggccacc gctgtaatta tccagaccga
    13321 tatgaatttt accgtcgagg aacgtcccaa cggtgagcac gacggctttg gcacggaact
    13381 tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata agatcttcaa
    13441 ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt accgcctgac
    13501 ggtagagcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct ttgcttgcgt
    13561 ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga ccgccgagtg
    13621 catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg ttgcagctca
    13681 tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc atacgcgccg
    13741 cggccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg tcaaaaggat
    13801 ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt gaagcccggg
    13861 ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg gtattaaaaa
    13921 gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat cgcactgccc
    13981 tgtggataac aaggatccgg cttttaagat caacaacctg gaaaggatca ttaactgtga
    14041 atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat acacaactca
    14101 aaaactgaac aaccgttgtt ccttggataa ctaccggttg atccaagctt cctgacagag
    14161 ttatccacag tagatcgcac gatctgtata cttatttgag taaattaatc cacgatccca
    14221 gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc ttcagtgttt
    14281 cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg atagccccac
    14341 aaaaggtgtc atattcacga ctaccaatac cgattgcgcc aaagcggacc gcagaaagat
    14401 cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga atatctccgg
    14461 caccgtgggt ggagctgata atcagccaga tccccgaggc agataaatct tccaacagcg
    14521 gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc aggtgttctg
    14581 ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct gccataaacc
    14641 gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc tacctgtgga
    14701 aaagtatggg attaaaaaag ccgatcatgg cttgatagta cgcatgatcg ggttttgcag
    14761 gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt tgataagtac
    14821 atgctggaga gcgtcgatcg aacggcacat tacttttata aagatgctgt agtggcctgt
    14881 tgtgtaatag gcttcagtga cttcatcaag gctttccagc tttgccagcg cggaagggta
    14941 gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac cgagctgctt
    15001 cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt tctctactcg
    15061 aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt aagcggtgcg
    15121 cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca gataattttc
    15181 cataggattt tcttatgcag attgatgatt cattctattt tagctttctt ttttaatgaa
    15241 tcaaaagtgt gttaggcttt ttattgaatg attattgcat gtgtgtcggt ttttgttgct
    15301 taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca ttgccaaaca
    15361 acgccaaatt agcttcgtga aatctcactt ttctcgtcaa ctggaagaac gtctggggct
    15421 gatcgaagtc caggcaccga ttcttagccg tgtgggggat ggcacgcagg ataacttgtc
    15481 gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc agttcgaagt
    15541 ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact tcagcgcggg
    15601 cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc tttctccgtt
    15661 gcactcggtc tatgtagacc agtgggactg ggaacgcgta atgggcgacg gtgagcgtca
    15721 attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag caaccgaagc
    15781 tgcggttagc gaagagtttg gcctggcacc gttcctgccg gatcagatcc acttcgtaca
    15841 cagccaggag ttactgtctc gttatccgga tctcgatgcc aaagggcgtg agcgggcgat
    15901 agcgaaagat cttggcgcgg tattccttgt cgggattggc ggcaagctga gcgatggtca
    15961 tcgccacgac gtgcgcgcac cggattatga tgactggagc accccgtcag agctgggtca
    16021 tgcgggtctg aacggcgata ttctggtgtg gaaccctgta ctggaagatg cgtttgagct
    16081 ttcttccatg gggatccgcg tggatgccga cacgctgaag catcagctgg cgctgaccgg
    16141 tgatgaagat cgtctgcagc tggaatggca tcaggcgctg ctgcgcggtg aaatgccgca
    16201 gaccatcggc ggcggtatcg gccagtctcg tttgactatg ctgctgctgc aactgccgca
    16261 tatcggccag gttcagtgtg gagtatggcc agctgctgtt cgcgagagcg tcccttctct
    16321 gctgtaataa tttatcgccg ccagcgtctg agcaggtggc ttcgcatccc ggtatcaaag
    16381 cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga catcgccacg
    16441 gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt cacgtcgtca
    16501 ggcaaccgct gagcgataaa atcagaaatc accaccgcat cggcatcaaa ccattccctg
    16561 ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc gccacgaaac
    16621 tgctggctta aaaaacggat tgcttgttcg atgccttgtg ggcctgaaag ctcataacgg
    16681 acgatctcgg tggaaaatag cataatatag cagcgccggt tttctgcgag agcaatgcgc
    16741 atcaaggcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat tgagccggaa
    16801 gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt atgtaccacc
    16861 ggacgttcga tcactttttc acgccacgac tcaccgtgca ggcgataggt gagcaactgt
    16921 ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag tgtcgccagt
    16981 tctggcggca gaagacgtaa aatatcatcg ctttgttgca gaccatcaac ctgctcagga
    17041 accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctgcgcatc gttgcgcggt
    17101 attgatttgg cttcccgaga acgccccagc tgctctgcca ggcgtttcag ttccggctgt
    17161 tcgttaagaa attcaccgta tttcacaatc aactgatagt cgccacgttt aagctgaccg
    17221 gcgctcatat cccacagatg accagctgca gtattgttat ctgcgagaat cggttcaagt
    17281 tgtccgctca gcgtcatgcg ttcctgaact tcactcaaca gttgttcgcg ttcttcttct
    17341 aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca acgctggaga
    17401 aaaagcgtgt gtaacgctga agtgatcgtg ctgttagcat caaccaactg tcgggcttgt
    17461 tctgcccatg gagaattcag acgatgcagt aagtccagga tctgtggtag ctgcataata
    17521 aactgtggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc ggtgagttcc
    17581 ggcgggactc gggcatcttt cagccgactg cgcagcgctt cacgccagcg gggaacatca
    17641 tcagtgattg ccgccttcag gcgtgggaat ttttcaaaga agaccgccag ctgcggtgag
    17701 gccagcagtg cgatgatcat ctcttcgatc aatccctctt cgctgacggc cagcatcaca
    17761 ttaagcgtat ccagcgttag cattgttgtg cctggcgaat ctgtgcaccg acatcctgta
    17821 ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt tgctgttcgc
    17881 tgaataaagc gtgttgcttg cgccagtcgc tttcaagctc ttccagttgt tgttttattt
    17941 caccaggcag accttcagcc gatgagccgg gaagtgccag cgtactgcct tgtaaactta
    18001 catcgcgaac gacaagatgt tgggcgctat caacttccag attcagtttc tgggcaaagc
    18061 cgataccgtt cagtttaccg cgaatttcac cacctttgct cagccactgt tccagcgcgc
    18121 tacgctcaaa ggagatatga accacttcca tatcatgcag ttttaacggt ttttgcagca
    18181 gtagagtcag agtggaagca gtaacgttaa caggaagttg atactgctga cgacggctga
    18241 aaatgccgcc cagacgaatt accgttaagg ctgttttatc gctttgttgc tgctggagtt
    18301 gcaggtgacg ttgcacaatc gcgccaaggc gagtcaacat cccttgctgt tgccaggcgt
    18361 gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc gcgtcatacc
    18421 acaggcaatc tttcagcaaa atgagatcta ccggggcaac agcactgcga ctgctaaaaa
    18481 aggcgctggc ctgcaataat cggatcgctt ttttccagcg acgatccgag acataaggcg
    18541 catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat acatgatcgg
    18601 gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct gtgacctgca
    18661 aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg gagcggaaat
    18721 tcgctttatc ctgcacttta tctaaccaca gacgaatcag catgcggtca tataacgctt
    18781 ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagcaga cgcatcggga
    18841 ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg agcaaggtat
    18901 taagaattgc cgggcccgct ttccagatct catccagaaa gacaatttct gcttccggca
    18961 ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga atagaaaggg
    19021 gaccaaaaac ttcttccggc gtggagaagc gggtcatcag atattcaaac gcgcgggcat
    19081 tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct ggcgggccaa
    19141 ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg gcgtggctac
    19201 gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc gctaataaat
    19261 gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa attgcgagta
    19321 tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg gggtattttt ctttgagcca
    19381 ggttaatgtg gccgcattta ggagtacgat tttgccgtta atcgtgcata ctgtgcgctt
    19441 ttttgtgggc caagggacta agcacacatt tcatatttca acgaaagact agtctatgag
    19501 cactgataat aagcaatcat tgcccgcgat taccctcgcg gcgattggag ttgtctacgg
    19561 cgatattggt accagcccgt tatatacact tcgtgaatgt ttgtccggcc agtttggttt
    19621 tggcgttgaa cgcgatgccg tgtttggctt tttatcgctg atcttctggc tgctaatctt
    19681 tgtggtttcc attaaatatc tcaccttcgt gatgcgggca gataacgccg gtgagggggg
    19741 gatcctgacg ttgatgtcgc ttgccgggcg taatacgtcg gcgcgaacca catcaatgct
    19801 ggtgattatg gggctaatcg gcggcagctt tttctatggt gaagtcgtca taacacccgc
    19861 tatttcggtg atgtcagcca ttgaaggtct ggaaatcgtc gccccgcagc tggatacctg
    19921 gatagttccc ctctcaatta tcgttctcac tttattattt atgattcaaa aacatggcac
    19981 ggctatggtc ggtaagctgt ttgcgccgat catgctgacc tggtttttga ttctggcagg
    20041 actggggtta cgtagcatta ttgctaaccc ggaagtgctg catgcgctga atccaatgtg
    20101 ggcggtgcat ttcttccttg aatacaaaac ggtttctttt attgcattag gggcagtggt
    20161 gttgtcgatt acgggggtcg aggcgctgta tgctgatatg gggcactttg gtaagttccc
    20221 tattcgcctg gcgtggttca ccgtcgtatt accttccttg acccttaatt acttcggtca
    20281 gggagcgctg ttgttaaaga acccggaagc gattaagaac ccgttcttcc tgttggcacc
    20341 ggactggtcg ctgatcccgc tgctgatcat cgccgcactg gcgacggtaa ttgcctcgca
    20401 ggcggttatc tctggcgtct tctcattgac gcgtcaggcg gtacgtctgg gatatttgtc
    20461 gccgatgcgc attattcaca cctccgaaat ggagtcaggg caaatctata ttccgtttgt
    20521 gaactggatg ctctatgtcg cggtcgtgat tgtgattgtc agctttgagc actccagcaa
    20581 cctggcggcg gcgtacggga ttgcggtgac cggaaccatg gtgctgacgt ctattctctc
    20641 gactaccgtg gcacgtcaga actggcactg gaataagtat tttgttgcgc tgatcctgat
    20701 tgctttcctt tgtgtcgata ttccattgtt caccgctaac ctcgataaac tgctctccgg
    20761 cggctggttg ccattgagcc tcggtactgt gatgtttatc gtgatgacca cctggaagag
    20821 cgagcgtttc cgcttgctgc ggcggatgca tgaacatggt aactctctgg aagcgatgat
    20881 tgcttcgctg gagaaatcac cgcctgttcg cgtgcccggg accgcggtgt atatgtcgcg
    20941 tgcaatcaac gtcattccct ttgcgctgat gcataacctt aaacataaca aggtattgca
    21001 tgagcgggtg attctgttaa ctctgcgtac cgaagacgcg ccatatgtcc ataatgtccg
    21061 tcgggtacag attgaacaac tgtcgcccac tttctggcgc gtggtggcaa gttatggttg
    21121 gcgagaaacg ccaaacgtgg aagaagtttt ccaccgctgc ggtctggaag gattaagttg
    21181 ccggatgatg gaaacctcct tctttatgtc gcatgagtcg ttgatcctcg gcaaacgccc
    21241 gtggtatttg cgtctgcgcg gcaagctgta cttgctgctg caacgtaatg cgctgcgtgc
    21301 accagatcaa tttgaaatcc cgccaaacag ggttatcgaa ctgggtactc aggtcgaaat
    21361 ctaacgccag acgcctcctt tctccataag ggggcgtttt tgttttcatg gttaatcacc
    21421 atgtaaaacg tttcgaggtt gatcacattt ccgtaacgtc acgatggttt tcccaactca
    21481 gtcaggatta aactgcgggt cagcgaaacg tttcgctgat ggagaaaaaa atgaaaaaag
    21541 gcaccgttct taattctgat atttcatcgg tgatctcccg tctgggacat accgatacgc
    21601 tggtggtgtg tgatgctggt ttacccatcc ccaaaagtac aacgcgtatc gatatggcat
    21661 taacccaggg tgtaccttct tttatgcagg tgctgggcgt cgtcacaaat gaaatgcagg
    21721 tcgaggctgc cattatcgcg gaagagatca aacaacataa tccgcaactc cacgaaacgt
    21781 tgctcactca ccttgagcag ctgcaaaaac accagggaaa taccattgaa attcgttaca
    21841 ccacgcatga acaattcaaa caacaaaccg cagaaagtca ggcggtaatt cgcagcggag
    21901 aatgttctcc gtatgcgaat atcattctct gtgctggcgt gacgttctga ggccgtcatg
    21961 gaagcattac ttcagcttaa aggcatcgat aaagccttcc cgggcgtaaa agccctctcg
    22021 ggcgcagcgt taaatgtcta tccgggccgc gtgatggcgc tggtgggcga aaacggcgcg
    22081 ggtaaatcca ccatgatgaa agtgcttact ggtatctatg ctcgcgatgc cggcacgctt
    22141 ttatggctgg ggaaagaaac gacatttacc gggccgaaat cttcccagga agccgggatt
    22201 gggattatcc atcaggaact gaacctgatc ccgcagttga ccattgccga aaacattttc
    22261 ctcggtcgtg agtttgttaa tcgctttggc aaaattgact ggaaaaccat gtatgccgaa
    22321 gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagct ggtgggcgat
    22381 ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt tgagtcgaaa
    22441 gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga atccctgttc
    22501 cgcgtcatcc gcgagctgaa atcgcaaggc cgcggtattg tctatatctc ccaccgcatg
    22561 aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgacgggca atttattgct
    22621 gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt gggccgcaag
    22681 ctggaagatc aatatccaca cctggacaaa gcgccgggag atatccgcct gaaagtcgat
    22741 aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg cgaaattctt
    22801 ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct ctacggcgca
    22861 ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac ccgttcaccg
    22921 caggatggcc tggcaaacgg cattgtgtat atctccgaag accgtaaacg tgacggttta
    22981 gtgttgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta cttcagccgc
    23041 gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt cattcgtctg
    23101 tttaatgtga aaacaccgtc gatggaacag gcaattggtc tgctttccgg tggcaatcag
    23161 caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtgttgat ccttgatgaa
    23221 cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat taaccagttc
    23281 aaagccgatg gcttgagcat cattctggtg tcatcggaga tgccagaagt attaggcatg
    23341 agcgatcgca tcatcgtcat gcatgaaggg catctcagcg gggaatttac tcgtgagcag
    23401 gccacccagg aagtgttaat ggctgccgct gtgggcaagc ttaatcgcgt gaatcaggag
    23461 taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag cgtggctgat
    23521 ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga cgttaagccc
    23581 gaactttttc accatcaata acttattcaa tattctccag caaacctcgg tgaacgccat
    23641 tatggcagtc gggatgacgc tggtgatcct gacgtcgggc atcgacttgt cggtaggttc
    23701 tctgttggcg ctgaccggcg cagttgctgc atctatcgtc ggcattgaag tcaatgcgct
    23761 ggtggctgtc gctgccgctc tcgcgttagg tgccgcaatt ggtgcggtaa ccggggtgat
    23821 tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc ttttactgcg
    23881 cggtgtgacc atggtttata ccaacggtag cccagtgaat accggcttta ctgagaacgc
    23941 cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacac cagtctggat
    24001 catgggaatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc tggggcgtta
    24061 catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca acgtcaataa
    24121 aatcaaaatc atcgtctatt ctctttgtgg tctgctggca tcgctggccg ggatcattga
    24181 agtggcgcgt ctctcctccg cacaacccac ggcggggact ggctatgagc tggatgctat
    24241 tgctgcggtg gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca ttgttgggac
    24301 gttgatcggc gcattaattc ttggcttcct taataatgga ttgaatttgt taggtgtttc
    24361 ctcctattac cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc tggtagacaa
    24421 caaaaagcag taataacgac tacaggacat cttgaatatg aacatgaaaa aactggctac
    24481 cctggtttcc gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg caaaagacac
    24541 catcgcgctg gtggtctcca cgcttaacaa cccgttcttt gtatcgctga aagatggcgc
    24601 gcagaaagag gcggataagc ttggctataa cctggtggtg ctggactccc agaacaaccc
    24661 ggcgaaagag ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa ttctgctgat
    24721 taacccgacc gactccgacg cagtaggtaa tgctgtgaag atggctaacc aggcgaacat
    24781 cccggttatc actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc acattgcttc
    24841 tgataacgta ctgggtggca aaatcgccgg tgattacatc gcgaagaaag cgggtgaagg
    24901 tgccaaagtt atcgagctgc aaggcattgc tggtacatcc gcagcccgtg aacgtggcga
    24961 aggcttccag caggccgttg ctgctcacaa gtttaatgtt cttgccagcc agccagcaga
    25021 ttttgatcgc actaaaggtt tgaacgtaat gcagaacctg ttgaccgctc atccggatgt
    25081 tcaggctgta ttcgcgcaga atgatgaaat ggcgctgggc gcgctgcgcg cactgcaaac
    25141 tgccggtaaa tcggatgtga tggtcgtcgg atttgacggt acaccggatg gcgaaaaagc
    25201 ggtgaatgat ggcaaactag cagcgactat cgctcagcta cccgatcaga ttggcgcgaa
    25261 aggcgtcgaa accgcagata aagtgctgaa aggcgagaaa gttcaggcta agtatccggt
    25321 tgatctgaaa ctggttgtta agcagtagtt ttaatcaggt tgtatgacct gatggtgaca
    25381 taaatacgtc atcgacagat gaacgtgtaa tataaagaaa agcagggcac gcgccaccct
    25441 aacacggtgg cgcattttat ggacatcccg aatatgcaaa acgcaggcag cctcgttgtt
    25501 cttggcagca ttaatgctga ccacattctt aatcttcaat cttttcctac tccaggcgaa
    25561 accgtaaccg gtaaccacta tcaggttgca tttggcggca aaggcgcgaa tcaggctgtg
    25621 gctgctgggc gtagcggtgc gaatatcgcg tttattgcct gtacgggtga tgacagcatt
    25681 ggtgagagcg ttcgccagca gctcgccact gataacattg atattactcc ggtcagcgtg
    25741 atcaaaggcg aatcaacagg tgtggcgctg atttttgtta atggcgaagg tgagaatgtc
    25801 atcggtattc atgccggcgc taatgctgcc ctttccccgg cgctggtgga agcgcaacgt
    25861 gagcgtattg ccaacgcgtc agcattatta atgcagctgg aatcaccact cgaaagtgtg
    25921 atggcagcgg cgaaaatcgc ccatcaaaat aagactatcg ttgcgcttaa cccggctccg
    25981 gctcgcgaac ttcctgacga actgctggcg ctggtggaca ttattacgcc aaacgaaacg
    26041 gaagcagaaa agctcaccgg tattcgtgtt gaaaatgatg aagatgcagc gaaggcggcg
    26101 caggtactgc atgaaaaagg tatccgtact gtactgatta ctttaggaag tcgtggtgta
    26161 tgggctagcg tgaatggtga aggtcagcgc gttcctggat tccgggtgca ggctgtcgat
    26221 accattgctg ccggagatac ctttaacggc gcgttaatca cggcgttgct ggaagaaaaa
    26281 ccattgccag aggcgattcg gtttgcccat gctgccgctg cgattgccgt aacacgtaaa
    26341 ggcgcacaac cttccgtacc gtggcgtgaa gagatcgacg catttttaga caggcagagg
    26401 tgacgcttgg ctacaatgaa agatgttgcc cgcctggcgg gcgtttctac ctcaacagtt
    26461 tctcacgtta tcaataaaga tcgcttcgtc agtgaagcga ttaccgccaa agttgaagcg
    26521 gcgattaaag aactcaatta cgcgccatca gccctggcgc gtagcctcaa actcaatcaa
    26581 acacatacca ttggcatgtt gatcactgcc agtaccaatc ctttctattc agaactggtg
    26641 cgtggcgttg aacgcagctg cttcgaacgc ggttatagtc tcgtcctttg caataccgaa
    26701 ggcgatgaac agcggatgaa tcgcaatctg gaaacgctga tgcaaaaacg cgttgatggc
    26761 ttgctgttac tgtgcaccga aacgcatcaa ccttcgcgtg aaatcatgca acgttatccg
    26821 acagtgccta ctgtgatgat ggactgggct ccgttcgatg gcgacagcga tcttattcag
    26881 gataactcgt tgctgggcgg agacttagca acgcaatatc tgatcgataa aggtcatacc
    26941 cgtatcgcct gtattaccgg tccgctggat aaaactccgg cgcgcctgcg gttggaaggt
    27001 tatcgggcgg cgatgaaacg tgcgggtctc aacattcctg atggctatga agtcactggt
    27061 gattttgaat ttaacggcgg gtttgacgcc atgcgccaac tgttatcaca tccgctgcgt
    27121 cctcaggccg tctttaccgg aaatgacgct atggctgttg gcgtttacca ggcgttatat
    27181 caggcagagt tacaggttcc gcaggatatc gcggtgattg gctatgacga tatcgaactg
    27241 gcaagcttta tgacgccacc attaaccact atccaccaac cgaaagatga actgggggag
    27301 ctggcgattg atgtactcat ccatcggata acccagccga cccttcagca acaacgatta
    27361 caacttactc cgattctgat ggaacgcggt tcggcttaga tttacgctgt cttttgatca
    27421 aattattacc atcggttgtt ttcagaagca tgaacattgc tgctgaagca acagtaataa
    27481 tgcccatcgt gataaacgta tagtggaatt gttcgacagt cgttgtgcct tccatccctt
    27541 cataaacgcg aaggacggcc gcacttacag caacgcctaa actaatcgac agttgctgcg
    27601 tgaccgccag aacactgtta ccgctgctgg cgttgtcatc ggtcagatcg gcaagtgtga
    27661 tggtattcat cgcggtaaat tgcgtcgaca tagccatccc taatataaac aacggcaaga
    27721 tcagcatcca tatcgccatt gccggtgatt gcaaagagaa ctgagcgatc attagcccaa
    27781 taatcaccgt gatccccact aacgtatggc gatagcccag acgacgtaag acttgggtaa
    27841 ccatcgattt tgcaataatg gaacctaacg ctgtcggtgc catcatacag ccggcaataa
    27901 acgcctgata accaaatcct acctgtaaca tcaatggcat aaggaacggt acacaaccgg
    27961 tccccagacg ggttgcaata ttgcctacta taccgatcga gaaagtgcgg gttttaaata
    28021 aatctaatga aattaatggg tttggcgtgt gtcgcgcatg gagaatatag agaagcagta
    28081 acccgatgct ggtgacaatt accgtcaagg caatccagct ggcgacaatc ttttccccga
    28141 atagctctat tccgcttgag aagagaacaa ggctgaggcc aaacagcaaa aagccagtga
    28201 tatcgaatct gcgtcgtgcg gtggtgaaat tgggcatatg tttgcgcgcg taaagaaggc
    28261 ccgcaatacc tatggggata ttgattaaaa atatccagtg ccaggttgcc caggtgacca
    28321 gcacgccgcc aagaacgggg cctgaaattg gccccaccag acccggcatg gcgacaaaat
    28381 tcaatactgg aagaagttca ttacgaggat aagcgcgcag taaggccagc cgagcaacag
    28441 gcatcatcat tgcgccgcct atcccctgaa taacccggaa gacaaccagc tgtggtagcg
    28501 aattagaaag tgcgcaggcc agagaaccca atgtgaacag actcacggca agggtaaaaa
    28561 tgcgacgcgt accgaagcga tcggctagcc atccgcttac cggaataagc atcgccaccg
    28621 tcagcgtata actgatgatg gctgattgca tcgcgagagg agaacgatta aggctatgag
    28681 cgattgcggg taaggcggta ttcagaatag tggcatcaag tgcctgcatg aagaaggcca
    28741 tcgccgcgat ccacggcaaa cccgccatac tgcgcttctt tttatcgctc attcaatgtc
    28801 ctgttatcgg gttgtcactt atcaggtgag cgtagcagcg cctgacaagc tttaaatgcc
    28861 gcgtcgccat cgctttggac aatcgcatcg acaatcgcct ggtgcagatc cagctttatc
    28921 actgtgtcgc tggtaattga cgtgaagtaa gtgtgataaa ccgaatggaa tagcgaggcg
    28981 aatgaggtca aaaacggatt ggcgctcatt tcatagatat gctcatgcca ggccatatcg
    29041 acttcgatcc agcgttcacg gcgaaagttc tcttttaatg ccgccatttc ggccattaac
    29101 gtattgagat gcgccttctg ttccgcggtg ccaaccgttg cagccagtag gcaagcttgc
    29161 ggctccagac agatacgcat aaccagaaag tgatcgatga cctgatgaaa gttctcttct
    29221 gtcatccacc aggtaagcaa ttcctgatca agaaaattcc agtttgattg tggcatgacc
    29281 cgggtaccaa ttcgcggtcg cggtaaaacc atcccttttg ccgttaacgt tttgaccgct
    29341 tcgcgtaccg ctgtacgact cactccaaat tgctcgccca gctcaatctc accaggcaaa
    29401 atggtgccgg gttcatattc accttttaag atgcgttgcg ccagcttctc agccagaaca
    29461 tacgaaaggt ttttctgtgc agctaactgt tgtgcgctta aaggcattac taatcttcct
    29521 ttttcttttt attcctcctt agtatgccac caggaagtgt gattacggtt gcaaaaacgg
    29581 caaattgctt gttttatggc acattaacgg ggcttttgct gaaaaaatgc gcggtcagaa
    29641 aattatttta aatttcctct tgtcaggccg gaataactcc ctataatgcg ccaccactga
    29701 cacggaacaa cggcaaacac gtcgccgggt cagcggggtt ctcctgagaa tctcaacaga
    29761 gaaaagcaaa aaatgcttga ctctgtagct ggaaagcgta ttatgcacac cccgcgccgc
    29821 tgagaaaaag cgaagcgg
//
LOCUS       DAZKHC020000052        29721 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZKHC020000052 DAZKHC020000000
VERSION     DAZKHC020000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.052"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(252..2321)
                     /gene="glyS"
                     /locus_tag="ACHOSM_RS13460"
                     /old_locus_tag="pgaptmp_002692"
     CDS             complement(252..2321)
                     /gene="glyS"
                     /locus_tag="ACHOSM_RS13460"
                     /old_locus_tag="pgaptmp_002692"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGU4559187.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFTEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2331..3242)
                     /gene="glyQ"
                     /locus_tag="ACHOSM_RS13465"
                     /old_locus_tag="pgaptmp_002693"
     CDS             complement(2331..3242)
                     /gene="glyQ"
                     /locus_tag="ACHOSM_RS13465"
                     /old_locus_tag="pgaptmp_002693"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGU4559188.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3337..3636)
                     /gene="ysaB"
                     /locus_tag="ACHOSM_RS13470"
                     /old_locus_tag="pgaptmp_002694"
     CDS             complement(3337..3636)
                     /gene="ysaB"
                     /locus_tag="ACHOSM_RS13470"
                     /old_locus_tag="pgaptmp_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HGU4559189.1"
                     /translation="MMMNAFFPAMALVVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3811..4806
                     /gene="wecH"
                     /locus_tag="ACHOSM_RS13475"
                     /old_locus_tag="pgaptmp_002695"
     CDS             3811..4806
                     /gene="wecH"
                     /locus_tag="ACHOSM_RS13475"
                     /old_locus_tag="pgaptmp_002695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HGU4559190.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHELRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4848..5285)
                     /gene="yiaA"
                     /locus_tag="ACHOSM_RS13480"
                     /old_locus_tag="pgaptmp_002696"
     CDS             complement(4848..5285)
                     /gene="yiaA"
                     /locus_tag="ACHOSM_RS13480"
                     /old_locus_tag="pgaptmp_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HGU4559191.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5331..5672)
                     /gene="yiaB"
                     /locus_tag="ACHOSM_RS13485"
                     /old_locus_tag="pgaptmp_002697"
     CDS             complement(5331..5672)
                     /gene="yiaB"
                     /locus_tag="ACHOSM_RS13485"
                     /old_locus_tag="pgaptmp_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HGU4559192.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5841..7295)
                     /gene="xylB"
                     /locus_tag="ACHOSM_RS13490"
                     /old_locus_tag="pgaptmp_002698"
     CDS             complement(5841..7295)
                     /gene="xylB"
                     /locus_tag="ACHOSM_RS13490"
                     /old_locus_tag="pgaptmp_002698"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HGU4559193.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7367..8689)
                     /gene="xylA"
                     /locus_tag="ACHOSM_RS13495"
                     /old_locus_tag="pgaptmp_002699"
     CDS             complement(7367..8689)
                     /gene="xylA"
                     /locus_tag="ACHOSM_RS13495"
                     /old_locus_tag="pgaptmp_002699"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HGU4559194.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            9055..10047
                     /gene="xylF"
                     /locus_tag="ACHOSM_RS13500"
                     /old_locus_tag="pgaptmp_002700"
     CDS             9055..10047
                     /gene="xylF"
                     /locus_tag="ACHOSM_RS13500"
                     /old_locus_tag="pgaptmp_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4559195.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            10125..11666
                     /gene="xylG"
                     /locus_tag="ACHOSM_RS13505"
                     /old_locus_tag="pgaptmp_002701"
     CDS             10125..11666
                     /gene="xylG"
                     /locus_tag="ACHOSM_RS13505"
                     /old_locus_tag="pgaptmp_002701"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HGU4559196.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11644..12825
                     /gene="xylH"
                     /locus_tag="ACHOSM_RS13510"
                     /old_locus_tag="pgaptmp_002702"
     CDS             11644..12825
                     /gene="xylH"
                     /locus_tag="ACHOSM_RS13510"
                     /old_locus_tag="pgaptmp_002702"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HGU4559197.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12903..14081
                     /gene="xylR"
                     /locus_tag="ACHOSM_RS13515"
                     /old_locus_tag="pgaptmp_002703"
     CDS             12903..14081
                     /gene="xylR"
                     /locus_tag="ACHOSM_RS13515"
                     /old_locus_tag="pgaptmp_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HGU4559198.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14189..15013)
                     /gene="bax"
                     /locus_tag="ACHOSM_RS13520"
                     /old_locus_tag="pgaptmp_002704"
     CDS             complement(14189..15013)
                     /gene="bax"
                     /locus_tag="ACHOSM_RS13520"
                     /old_locus_tag="pgaptmp_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HGU4559199.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15333..17363
                     /gene="malS"
                     /locus_tag="ACHOSM_RS13525"
                     /old_locus_tag="pgaptmp_002705"
     CDS             15333..17363
                     /gene="malS"
                     /locus_tag="ACHOSM_RS13525"
                     /old_locus_tag="pgaptmp_002705"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU4559200.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTQPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASQVRVCPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAEQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17541..18794
                     /gene="avtA"
                     /locus_tag="ACHOSM_RS13530"
                     /old_locus_tag="pgaptmp_002706"
     CDS             17541..18794
                     /gene="avtA"
                     /locus_tag="ACHOSM_RS13530"
                     /old_locus_tag="pgaptmp_002706"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HGU4559201.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18945..19418)
                     /gene="ysaA"
                     /locus_tag="ACHOSM_RS13535"
                     /old_locus_tag="pgaptmp_002707"
     CDS             complement(18945..19418)
                     /gene="ysaA"
                     /locus_tag="ACHOSM_RS13535"
                     /old_locus_tag="pgaptmp_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU4559202.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19520..20368)
                     /gene="yiaJ"
                     /locus_tag="ACHOSM_RS13540"
                     /old_locus_tag="pgaptmp_002708"
     CDS             complement(19520..20368)
                     /gene="yiaJ"
                     /locus_tag="ACHOSM_RS13540"
                     /old_locus_tag="pgaptmp_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HGU4559203.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20569..21567
                     /gene="yiaK"
                     /locus_tag="ACHOSM_RS13545"
                     /old_locus_tag="pgaptmp_002709"
     CDS             20569..21567
                     /gene="yiaK"
                     /locus_tag="ACHOSM_RS13545"
                     /old_locus_tag="pgaptmp_002709"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HGU4559204.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWSNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21579..22046
                     /gene="yiaL"
                     /locus_tag="ACHOSM_RS13550"
                     /old_locus_tag="pgaptmp_002710"
     CDS             21579..22046
                     /gene="yiaL"
                     /locus_tag="ACHOSM_RS13550"
                     /old_locus_tag="pgaptmp_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGU4559205.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            22164..22637
                     /gene="yiaM"
                     /locus_tag="ACHOSM_RS13555"
                     /old_locus_tag="pgaptmp_002711"
     CDS             22164..22637
                     /gene="yiaM"
                     /locus_tag="ACHOSM_RS13555"
                     /old_locus_tag="pgaptmp_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HGU4559206.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            22640..23917
                     /gene="yiaN"
                     /locus_tag="ACHOSM_RS13560"
                     /old_locus_tag="pgaptmp_002712"
     CDS             22640..23917
                     /gene="yiaN"
                     /locus_tag="ACHOSM_RS13560"
                     /old_locus_tag="pgaptmp_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HGU4559207.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            23930..24916
                     /gene="yiaO"
                     /locus_tag="ACHOSM_RS13565"
                     /old_locus_tag="pgaptmp_002713"
     CDS             23930..24916
                     /gene="yiaO"
                     /locus_tag="ACHOSM_RS13565"
                     /old_locus_tag="pgaptmp_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HGU4559208.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAT
                     KKFNDLLPERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGTNPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSSEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            24920..26416
                     /gene="lyxK"
                     /locus_tag="ACHOSM_RS13570"
                     /old_locus_tag="pgaptmp_002714"
     CDS             24920..26416
                     /gene="lyxK"
                     /locus_tag="ACHOSM_RS13570"
                     /old_locus_tag="pgaptmp_002714"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HGU4559209.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            26413..27075
                     /gene="ulaD"
                     /locus_tag="ACHOSM_RS13575"
                     /old_locus_tag="pgaptmp_002715"
     CDS             26413..27075
                     /gene="ulaD"
                     /locus_tag="ACHOSM_RS13575"
                     /old_locus_tag="pgaptmp_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGU4559210.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            27068..27928
                     /gene="sgbU"
                     /locus_tag="ACHOSM_RS13580"
                     /old_locus_tag="pgaptmp_002716"
     CDS             27068..27928
                     /gene="sgbU"
                     /locus_tag="ACHOSM_RS13580"
                     /old_locus_tag="pgaptmp_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HGU4559211.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            27922..28617
                     /gene="araD"
                     /locus_tag="ACHOSM_RS13585"
                     /old_locus_tag="pgaptmp_002717"
     CDS             27922..28617
                     /gene="araD"
                     /locus_tag="ACHOSM_RS13585"
                     /old_locus_tag="pgaptmp_002717"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGU4559212.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDPPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(28649..28750)
                     /locus_tag="ACHOSM_RS13590"
                     /old_locus_tag="pgaptmp_002718"
                     /pseudo
     CDS             complement(28649..28750)
                     /locus_tag="ACHOSM_RS13590"
                     /old_locus_tag="pgaptmp_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            28754..29017
                     /locus_tag="ACHOSM_RS13595"
                     /old_locus_tag="pgaptmp_002719"
                     /pseudo
     CDS             28754..29017
                     /locus_tag="ACHOSM_RS13595"
                     /old_locus_tag="pgaptmp_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28964..29704)
                     /gene="yiaT"
                     /locus_tag="ACHOSM_RS13600"
                     /old_locus_tag="pgaptmp_002720"
     CDS             complement(28964..29704)
                     /gene="yiaT"
                     /locus_tag="ACHOSM_RS13600"
                     /old_locus_tag="pgaptmp_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HGU4559213.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
BASE COUNT         7332 a         7639 c         7441 g         7309 t
ORIGIN      
        1 ttgatttata gtcagatggg gcttttctct gtctgccttt cggtgaatac ctgagacaaa
       61 cagtctcaag cacccgtagc tattctagct taataagttt gtttcttctc cttgatataa
      121 ccctaaaaaa atctcataaa attaatatat gagataatct ttattcagca gaagattatt
      181 aaaggttgct gtattattta gcgataataa aaaaagcctg ccagatggca ggctttttaa
      241 taacggcgtt attactgcaa cagcgaaata tccgcaacgc gcaggaacag ttcgcgcagt
      301 ttctccagca tggtcagacg gttgagacgc aattctttgt catcaaccat taccatcact
      361 ttatcgaaga aagcatcaac cggttcacgc agctcagcca gttcgaccag cgcatcctgg
      421 taacgacctt ccgtaaagta cggctccagc ttgtcacgta gcaccacaac ctgcatcgcc
      481 agtttaattt cttccggctc tttcagagta gaggcattca cgcgatcgct cagcacttcg
      541 tcagatttcg ccaaaatgtt ggagacacgc ttgttcgccg ccgccagtgc agcagctgct
      601 tcaagggtac ggaagtgcga taccgctttc atgcgggcat cgaaatcagc cggacgagtc
      661 ggacgacgcg ccagtaccgc ctggatggtg tcaacggtat aaccttcgtc ctgataccag
      721 gcgcggaagc gaccgagcat aaagtcgata acatcatcaa ctacgttggc attagtcagc
      781 ttatcgccat acagacgcac cgcttcttcg gtcagcgttt gcagatcaag gttgaggttc
      841 ttctcaacga taattcgcag cacgccaagt gcggcacgac gcagcgcaaa cgggtctttg
      901 tcgcctttcg gatgctggcc gataccgaag atacccgcca gagtatccat cttgtcagca
      961 atcgccagcg cacaggctac cgggttagac ggcaggtcat caccggcaaa gcgcggctga
     1021 tactgctcat tcagcgccac cgcgacatct tccgcttcgc catcgtgacg cgcatagtgc
     1081 atccccataa cgccctgggt gtcggtgaac tcgaagacca tgttggtcat caggtcgcac
     1141 ttagacagca gacccgcacg ggtagcgtgg ttaacgtcag cgccaatctg ttcagcaatc
     1201 cagccagcca gcgcctggat gcggtcagtt ttgtcgcgca gcgtccccaa ctgttgctgg
     1261 aacaacacgg tttgcaggcg cggcaggtta tcttcaagac gttttttacg gtcggtgttg
     1321 aagaagaact cggcatccgc cagacgcgga cgaacgactt tctcgttacc ggagataatc
     1381 tgctgcggat ctttcgattc gatgttggca acgaagataa agttcggcag cagtttgccg
     1441 tcgttcgcat acaccgggaa gtatttctgg tcacctttca tggtgtaaac cagcgcttca
     1501 gccggcaccg cgaggaattt ctcttcgaat tttgcggtca gaacgaccgg ccactccacc
     1561 agcgaagcca cttcttccag caggctttcg cttaagtcag cgttaccgcc aatcttacgc
     1621 gctgcttctt cggcatcggc tttaatcttc gccttacgtt cttcgtaatc ggcgatgact
     1681 ttcccacgct cacgcagaat ttccggatac tgatcggcgt tatcgatggt gaattccggc
     1741 tcgcccataa agcggtggcc gcgaatcacg cgatcggact gaatgcccag aatggttgcc
     1801 ggaatgactt tgtcgcccag cagcagggtc acggtgtgca ccggacgcac gaagtgcacg
     1861 tcgcttgcgc cccaacgcat cagtttcggg atcggcagtt tcgccagaga agtcgcaacc
     1921 atattcggca gcagtgcttc ggtgctttcg cccttcacat gggcgcgata cagcagccat
     1981 tcgcctttat cggtagtcag acgctcagcc tggtcaacgg taataccgca accacgcgcc
     2041 caaccttctg ccgctttgct cggtttgcct tcagcgtcga acgcctgggc aatcgccggg
     2101 ccgcgttttt cgatttcacg atccggttgc gcttccgcca ggttagctac tttcagcgcc
     2161 agacgacgcg gagcagcaaa ccattgaacg gtgccgtgtg cgaggccagc gttatccagc
     2221 tccgcagtaa aattcgcagc aaaggactca gccaggctgc gcagtgcttt tggtggcagc
     2281 tcttcagtgc cgatttccac cagaaaagtt ttctcagaca tagccgcctc ttacttatct
     2341 ttgttgcaca tcgggaagcc gagggcttca cgggaagcgt agtatgcttc tgccactgct
     2401 ttggtcaggg tgcgaatgcg cagaatgtag cgctgacgct cggtgacgga gatggcttta
     2461 cgcgcatcca gcaggttgaa gctgtgggcg gctttcagaa tacgctcgta ggctggcagc
     2521 ggcagcggat tttccagcgc cagcagctgc tgcgcttctt tctcgtactg ctcaaagcag
     2581 gtgaacagga agtccacatc cgcgtattcg aagttgtaag tggactgctc cacttcgttc
     2641 tgatggaaca cgtcgccgta ggtggtttta cccagcgggc cgtcgctcca gaccaggtcg
     2701 taaacgctgt ctacgccctg aatgtacatg gccagacgtt ccagaccgta ggtgatctcg
     2761 ccggtaaccg gtttacactc cagaccacca acctgctgga agtaagtgaa ctgcgtcact
     2821 tccatgccgt ttagccacac ttcccagccc agtccccagg cacccagagt cgggttttcc
     2881 cagttatctt ccacgaaacg gatgtcgtga atagtcgggt ccatgcccag ctctttcaga
     2941 gaaccgaggt acagctcctg aatattgtcc ggcgatggct taatgaccac ctggaactga
     3001 tagtagtgct gtaaacggtt ggggttttcg ccgtagcgac catcggtcgg gcgacgagaa
     3061 ggctgaacat aagcagccgc catcggttct ggccccagcg cgcgcagaca ggtcattggg
     3121 tgagaggttc ccgcgccgac ttccatgtcc aatggttgaa caatggtgca gccctggcga
     3181 gcccagtaat cctgtaaggt caggatcaag ccctggaagg tcctggtatc aaacttttgc
     3241 atattatttc gtgctggata cgtgtggatt taaaggaagg gatcagtata cccgctggat
     3301 ggaagatata cagtacgaaa cgggaaaaag cagggcttaa cgcatggaaa gatgcaaaaa
     3361 atggccatct gcgtcaaaag aacagacaaa ctgctcttta cggaaggtat aaccgcgcat
     3421 ttcatagctt ccctggaact gttcgaaggc ggtgacgtcg attttttgct cgccggtgtt
     3481 ataacgtttt gccgcctgat ccttgcataa cgcttccata ttcaacgaac gcagaggatc
     3541 aacctttacc cgttgtgctt tctgcacggg tgacggtgta gaacaaccca ctagcaccac
     3601 aagcgccatt gccggaaaga aagcgttcat catcattaaa ttaccgtctg gtttgcaggt
     3661 gagtcttatt atttatatcg gtatagtggc tgatatctta cgaatttcct taagcctcta
     3721 taagctgtag tgataaaact cagattattc cagtgcgcca accaaatgat ttatcggtca
     3781 gacttttttt aaccagatac agagacacag atgcagccca aaatttactg gattgataac
     3841 ctgcgaggga tagcgtgttt aatggtggtg atgattcaca ccactacctg gtatgtgacc
     3901 aatgctcata gtgttagccc cgtcacatgg gatatcgcca atgttctgaa ttctgcctct
     3961 cgtgtcagcg tgccgctatt tttcatgatt tccggctatc tcttttttgg cgaacgcagc
     4021 gcccagccgc gccatttctt gcgtatcggc ttatgtctga ttttttatag cgcaatcgca
     4081 ctgctctaca ttgcgctgtt tacctccatc aatatggagt tagcgctgaa aaacctgctg
     4141 caaaagccag tgttttacca cttgtggttt ttcttcgcga ttgcggtgat ttatctggtt
     4201 tcaccgctga ttcaggtgaa gaacgtcggc ggaaaaatgt tgctggtgct aatggcggtg
     4261 attggcatta tcgctaaccc aaacacagtg ccgcagaaaa ttgacggttt tgaatggctg
     4321 ccaattaact tatatatcaa tggcgatact ttttactaca ttctgtatgg catgttgggc
     4381 cgcgctatag ggatgatgga cacacagcat aaagcactgt cgtgggtgag cgccgcgctg
     4441 tttgcgacgg gggtttttat tatctctcgc gggacattat atgaattgca gtggcgcgga
     4501 aattttgccg atacctggta tctttactgt gggccgatgg tttttatctg cgcaatcgcg
     4561 ctattgactc tggttaaaaa cacgctggat acgcgtacca ttcgcggact tggcttaatc
     4621 tcccgccatt cattgggtat atacggattc cacgccttga ttatccatga gctgcgcacc
     4681 cggggaattg agcttaaaaa ttggccaata ctggatatta tttggatctt ttgcgcgacg
     4741 ttggcagcga gtttgttact ttctatgctg gtacaacgaa tcgacagaaa cagattagtg
     4801 agttaagtaa aagcccgttc acattggact gaccgggctt acgtgaatta ttcgctgtat
     4861 tcttcctggg taacctgtgt ttcttttggc gggtttattc cggcatcacg aatattcttc
     4921 tgcaccgcta ctgcaccaaa caagcttaag aagaaagcca gtccataaaa acctttttca
     4981 ctgagtaata atgtcgcatt ccacagacct accatcagta atgcaacaga gataataaag
     5041 acagtcaggc aggtcatata ataaatggaa gtggtcggta tgccttcata cttgtcccga
     5101 acggtctttt gataagacgc agcagaaaac agtcctaaca ccagtacggc aaaataatac
     5161 cctttttcat ttaactgcat ctctgcattc catagcccta acagataggt aacgatacca
     5221 ccaacgagag ctatccatga cacaatacta aaggccggtg aataggttga tattttgttg
     5281 tccatcacag tattcctttc attcctgaat gtgtaagagc tttatgttgc tcataccgag
     5341 cgaaacaaac gcatttgacc aaacaagcag acaaaaaagg cgatggggta gatcaccatt
     5401 tcataagcat tgataggtgc atttaaaaca ccgataaaca agagaccgat agtgattaac
     5461 gcaactaact ggcagatatg ggtaaaaaaa ttatccagtt gcccgagttt ttctgcgcga
     5521 agatatgcat agttaccaaa agttgctgtc attaacacgc caagaaaata gccttttcca
     5581 cttagcaatg ggcatgagat ccatagccca accagataaa ccagcgcccc gacaacaaat
     5641 aatagttttg ccacagtttt tgaggttttc ataacgatct ccatatctac cagcgataca
     5701 ttacgagtaa ccaacgaaag acaaaactga aaaatgccat taacaaatga ttttcagaat
     5761 aaattcatac taaatattaa ttaattagtg agatatatag atgtgaatta tcccccaccc
     5821 ggtcaggcag gggataacgt ttacgccatt aatggcagaa gttgctgata gaggcgacgg
     5881 aacgtttcac gtcgtggctg ataagcggca taacgctgcg catctggtag atgcgactgt
     5941 tctaacggta gctgcggcaa caactcaatg agcgatttct ctggattcgc cgcaatctgc
     6001 gccagccttg ctgcgcccag tgctggcccc acatcccccc ccgtacggta atcgagctgc
     6061 tgaccgctga tatccgccag catctgacgc cagtactcac tacgcgcccc gccaccaatc
     6121 aacgtaacac tttgcggttt aatgccgcag gcatgcacga catccatgcc atctgccagc
     6181 gcataaccca cgccttccag cactgctcgc gccagttcat tggggccatg ttgatgagtc
     6241 aaaccaaaga aaaccccctt cgcctgggga ttattgtgtg gtgtacgctc gccggaaaga
     6301 taaggcagaa accaaactgg ctcgacactt tcatcagcct gttgagctgc agcgattaaa
     6361 gctgggacat tgcacaggcc ggttaatttc gcggcccaat ccagacacga tgctgcactc
     6421 agcattacag acattaaatg ccaacgctgc ggtagcgcat ggcaaaagct gtgtacagcg
     6481 ctttctggct tgcttaagaa cccttcgctg acagcaaaat agacccccga cgtccccagc
     6541 gataacattg cctgattagc atcaaccatt cccacaccaa ctgcaccagc tgcattgtcg
     6601 ccaccgcctg cgacaactgg caccgtcgcc ataccccacg ctttcgcaac ttcaggtaac
     6661 aaagcaccag taatttcgct gccttcgtat aatgcgggca tctggtcacg agataagtcg
     6721 caagcctgca gcatgacgtc actccagtca cgctttgcga catccagcca catggtgcca
     6781 gctgcgtcag acatatcgct ggcaaactcc cccgtcatac gcagacgcaa gtaatctttc
     6841 ggtaataata ctttgtctat ttgacggaat atctccggct catgccgctg aacccatagc
     6901 aatttaggcg cagtaaatcc gggcatcatc aggttgccgg taatcactcg tgattgcgga
     6961 actctcgctt ccagcaaagt gcactcttgc gcacagcgcc cgtcgttcca caaaatggca
     7021 gggcgcaata cccgttgttg agcatccagt aaggttgctc catgcatctg gccggcaata
     7081 cccaatgctt taacgtcctg cagagaatgc tgatcgccca gagctttcat tgcgcgatca
     7141 gttgcctgcc accactgttc cgggtcttgt tccgaccaga gtggatgcgg gcgcgaaacg
     7201 gtcagctttt ccgtttgcga agcaaccacc tcaccctgct cgttgagcaa aataactttt
     7261 acgcccgagg tgccaagatc tatcccgata tacatatcga tcgttcctta aaaaaatgcc
     7321 cggtatcgct accgataacc gggccaacgg actgcacagt tagccgttat ttgtcgaaca
     7381 gataatgatt taccagattt tccagttgct cctggcgacc actctgatgc accggagaca
     7441 aattatgttc ctgagcatat ttggctaaat ctgccagtga catttggcct ttcaggattt
     7501 gctggcccaa ttcgctattc cagccggaat aacgctgcgc gatgcgttta tccagctcgc
     7561 catcttcaat catgcgcgct gcaattttca gcgccagtgc catcgtatcc atcgcgccga
     7621 tatgaccgta aaacagatca tatttatcag tactttgacg acgtactttg gcatcgaagt
     7681 tcagaccacc ggtggtgaaa ccgcctgctt tgagaatttc atacatcacc agcgcattct
     7741 cttccacact gttcgggaac tggtcggtgt cccagcccag ttgcgcatcg ccacggttgg
     7801 cgtcgacaga accgaacagg ccaagcgcaa tggcggtggc tatttcatga tggaaagagt
     7861 gacctgccag cgtcgcgtgg ttagcttcaa tgttcagttt aatctctttt tccagaccaa
     7921 actgtttcag gaagccatag accgtcgcgg catcgtaatc atattgatgt ttagtcggtt
     7981 cttgcggttt cggttcgata agcaacgtgc cctggaagcc gattttatgt ttatgctcaa
     8041 ccaccatctg cataaagcgg cccagttgtt cacgctcctg acgcaagtcg gtatttaaca
     8101 gcgtttcgta accttcacga ccaccccaca ggacatagtt ttcaccgccc aatttatggg
     8161 ttgcttccat cgctgtaaca acttgcgttg ccgcccagct gaagacttca ggatctgggt
     8221 tcgtcgccgc acccgcgcca tagcgagggt ttgtaaagca gttagcggtt ccccacagca
     8281 gcttcacgcc gctctcttct tgcttggctg ccaggacatc aaccatttgc gcaaaattat
     8341 tgatgtactc ttttaacgac gcgccctcag gggaaacatc cacatcgtgg aagcaataaa
     8401 atggcacatg taacttgtgg aaaaactcaa atgcgacatc tgctttacgc ttcgccaacg
     8461 ccagtgcctc accaggctgc tgccacggac gattaaacgc ccccacacca aacatgtccg
     8521 ccccgttcca gcagaaggtg tgccagtagc aggcggcaaa acgcaagtgc tcttccatac
     8581 gcttacccaa caccagttcg tcgggattgt agtgacggaa tgctaacggg tttgaggatt
     8641 ttgagccttc ataacgaacg cgatcgagct ggtcaaaata ggcttgcata ttgaactcca
     8701 taatcaggta atgccgcggg tgatggatga tgtcgtaata ttgggcactc cctttcagtt
     8761 gctcaattat gttatttcac actgctattg agataattca caagtgtgcg ctcgctcgca
     8821 aaataaaatg ggatgatgaa actgggtaat tcctcgaaga taaaaatgca ataagtacaa
     8881 ttgcgcaaca aaagtaagat ctcggtcata aatcaagaaa taaaccaaaa atcgtaatcg
     8941 aaagataaaa atctgtaatt gttttcccct gtttagttgc taaaaattgg ttacgtttat
     9001 cgcggtgatt gttacttatt aaagctgtcc tctaactaca gaaggcccta caccatgaaa
     9061 ataaagaaca ttctactcac cctttgcacc tcacttctgc ttaccaacgt tgcggcacac
     9121 gccaaagaag tcaaaatagg tatggcgatt gatgatctcc gtcttgaacg ctggcaaaaa
     9181 gatcgagata tttttgtgaa aaaggcagaa tctctcggcg cgaaagtatt tgtacagtct
     9241 gcaaatggca atgaagaaac acaaatgtcg cagattgaaa acatgattaa ccggggtgtc
     9301 gatgttcttg tcattattcc gtataacggt caggtattaa gtaacgttgt aaaagaagcc
     9361 aaacaagaag gcattaaagt attagcttac gaccgtatga ttaacgatgc ggatatcgat
     9421 ttttatattt ctttcgataa cgaaaaagtc ggtgaactgc aggcaaaagc cctggtcgat
     9481 attgttccgc aaggtaatta cttcctgatg ggcggctcgc cggtagataa caacgccaag
     9541 ctgttccgcg ccggacaaat gaaagtgtta aaaccttacg ttgattccgg aaaaattaaa
     9601 gtcgttggtg accaatgggt tgatggctgg ttaccggaaa acgcattgaa aattatggaa
     9661 aacgcgctaa ccgccaataa taacaaaatt gatgctgtag ttgcctcaaa cgatgccacc
     9721 gcaggtgggg caattcaggc attaagcgcg caaggtttat cagggaaagt agcaatttcc
     9781 ggccaggatg cggatctcgc aggtattaaa cgtattgctg ccggtacgca aactatgacg
     9841 gtgtataaac ctattacatt gctggcaaat actgccgcag aaattgccgt tgaattgggc
     9901 aatggtcagg agccaaaagc agatacctca ctgaataatg gcctgaaaga tgtcccctcc
     9961 cgcctcctga caccgatcga tgtgaataaa aacaacatca aagatacggt aattaaagac
    10021 ggattccaca aagagagcga gctgtaagcg ttacgccccg gcgcggagcg ggggcgtgat
    10081 ttctctccat gccgcgtgaa ttaattggct taggtggagt cgttatgcct tatctacttg
    10141 aaatgaagaa cattaccaaa accttcggca gtgtgaaggc gattgataac gtcagcttgc
    10201 ggttgaatgc tggcgaaatc gtctcactct gtggggaaaa tgggtctggt aaatcaacgc
    10261 tgatgaaagt gctgtgtggt atttatcccc atggctccta cgaaggcgaa attatttttg
    10321 cgggagaaga gattcaggcg agtcacatcc gcgataccga acgcaaaggt atcgccatca
    10381 ttcaccagga attggccctg gtgaaagaat tgaccgtact ggaaaatatc ttcctgggta
    10441 acgaaataac ccacaatggt attatggatt atgacctgat gacgctacgc tgtcagaagc
    10501 tgctcgcaca ggtcagttta tccatttcac cagatacccg cgtgggagat ttagggcttg
    10561 ggcaacaaca actggttgaa atagccaagg cacttaataa acaggtgcgc ttgttaattc
    10621 tcgatgaacc gacagcctca ttaactgagc aggaaacgtc ggttttactg gatattattc
    10681 gcgatctaca acagcacggt atcgcctgta tttatatttc gcacaaactc aacgaagtca
    10741 aagcgatttc cgatacgatt tgcgttattc gcgacggtca gcacattggt acgcgtgatg
    10801 ctgccggaat gagtgaagac gatattatca ccatgatggt cgggcgagag ttaaccgcgc
    10861 tttaccctaa tgaaccacat accaccggag atgaaatatt acgtattgaa catctgacgg
    10921 catggcatcc ggttaatcgt catattaaaa gagttaatga tgtctcgttt tccctgaaac
    10981 gtggcgaaat actgggtatt gccggactcg ttggtgccgg acgtaccgag accattcagt
    11041 gcctgtttgg cgtgtggccc ggacaatggg aaggaaaaat ttatattgat ggcaaacagg
    11101 tagatattcg taactgtcag caagccatcg cccaggggat tgcgatggta cccgaagaca
    11161 gaaagcgcga cggcatcgtt ccggtaatgg cggttggtaa aaatattacc ctcgccgcac
    11221 tcaataaatt taccggtggc attagccagc ttgatgacgc ggcagagcaa aaatgtattc
    11281 tggaatcaat tcagcaactc aaagttaaaa cgtcgtcccc cgaccttgct attggacgtt
    11341 tgagcggcgg caatcagcaa aaagcgatcc tcgctcgctg tctgttactt aacccgcgca
    11401 tcctcattct tgatgaacct accaggggta tcgatattgg cgcgaaatac gagatctaca
    11461 aattaattaa ccaactcgtc cagcagggta ttgccgttat tgtcatctct tccgaattac
    11521 ctgaagtgct cggccttagc gatcgggtac tggtgatgca tgaagggaaa ctaaaagcca
    11581 acctgataaa tcataacctg actcaggagc aggtgatgga agccgcattg aggagcgaac
    11641 atcatgtcga aaagcaatcc gtctgaagtg aaattggccg taccgacatc cggtggtttt
    11701 tctgggctga agtcactgaa tttgcaggtc ttcgtgatga ttgcagctat catcgcaatc
    11761 atgctgttct ttacctggac caccgatggt gcctacttaa gcgcccgtaa cgtctccaac
    11821 ctgttacgcc agaccgcgat taccggcatc ctcgcggtag gaatggtgtt cgtcataatt
    11881 tctgctgaaa tcgacctttc cgtcggctca atgatggggc tattaggtgg cgtcgcggcg
    11941 atttgtgacg tctggttagg ctggcctttg ccacttacca tcattgtgac gttggttctg
    12001 ggactgcttc tcggtgcctg gaacggatgg tgggtcgcgt accgcaaagt cccttcattt
    12061 attgtcaccc tcgcgggcat gttggcattt cgcggcatac tcattggcat caccaacggc
    12121 acgactgtat cccccaccag cgccgcgatg tcacaaattg ggcaaagcta tctccccgcc
    12181 agtaccggct tcatcattgg cgcgcttggc ttaatggctt ttgttggttg gcaatggcgc
    12241 ggaagaatgc gccgtcaggc tttgggttta cagtctccgg cctctaccgc agtagtcggt
    12301 cgccaggctt taaccgctat catcgtatta ggcgcaatct ggctgttgaa tgattaccgt
    12361 ggcgttccca ctcctgttct gctgctgacg ttgctgttac tcggcggaat gtttatggca
    12421 acgcggacgg catttggacg acgcatttat gccatcggcg gcaatctgga agcagcacgt
    12481 ctctccggga ttaacgttga acgcaccaaa cttgccgtgt tcgcgattaa cggattaatg
    12541 gtagccatcg ccggattaat ccttagttct cgacttggcg ctggttcacc ttctgcggga
    12601 aatatcgccg aactggacgc aattgcagca tgcgtgattg gcggcaccag cctggctggc
    12661 ggtgtgggaa gcgttgccgg agcagtaatg ggggcattta tcatggcttc actggataac
    12721 ggcatgagta tgatggatgt accgaccttc tggcagtata tcgttaaagg tgcgattctg
    12781 ttgctggcag tatggatgga ctccgcaacc aaacgccgtt cttgattttg ataaaaattt
    12841 tctcaaagcc ggttacgtat taccggtttt gagtttttgc atgattcagc aggaaaagaa
    12901 ccatgtttac taaacgtcac cgcatcacat tactgttcaa tgccaataaa gcctatgacc
    12961 ggcaggtagt agaaggcgta ggggaatatt tacaggcgtc acaatcggaa tgggatattt
    13021 tcattgaaga agatttccgc gcccgcattg ataaaatcaa ggactggtta ggagatggcg
    13081 tcattgccga cttcgacgac aaacagatcg agcaagcgct ggctgatgtc gacgtcccca
    13141 ttgttggggt tggcggctcg tatcaccttg cagaaagtta cccacccgtt cattacattg
    13201 ccaccgataa ctatgcgctg gttgaaagcg catttttgca tttaaaagag aaaggcgtta
    13261 accgctttgc tttttatggt cttccggaat caagcggcaa acgttgggcc actgagcgcg
    13321 aatatgcatt tcgtcagctt gtcgccgaag aaaagtatcg cggagtggtt tatcaggggt
    13381 tagaaaccgc gccagagaac tggcaacacg cgcaaaatcg gctggcagac tggctacaaa
    13441 cgctgccacc gcaaaccggg attattgccg ttactgacgc ccgagcgcgg catattctgc
    13501 aagtatgtga acatctacat attcccgtac cggaaaaatt atgcgtgatt ggcatcgata
    13561 acgaagaact gacccgctat ctgtcgcgtg tcgccctttc ttcggtcgct cagggcgcgc
    13621 ggcaaatggg ctatcaggcg gcaaaactgt tgcatcgatt attagataaa gaagaaatgc
    13681 cgctacagcg aattttggtc ccaccagttc gcgtcattga acggcgctca acagattatc
    13741 gctcgctgac cgatcccgcc gttattcagg ccatgcatta cattcgtaat cacgcctgta
    13801 aagggattaa agtggatcag gtactggatg cggtcgggat ctcgcgctcc aatcttgaga
    13861 agcgttttaa agaagaggtg ggtgaaacca tccatgccat gattcatgcc gagaagctgg
    13921 agaaagcgcg cagtctgctg atttcaacca ccttgtcgat caatgagata tcgcaaatgt
    13981 gcggttatcc atcgctgcaa tatttctact ctgtttttaa aaaagcatat gacacaacgc
    14041 caaaagagta tcgcgatgta aatagcgagg tcatgttgta gcgatgatga gaattgtcgg
    14101 cgtcacatca gggaactcag cacagccacc tgatgcgagc aaaagcctta tccgaccgac
    14161 aaatcagata aggctcaagg aaatgcaatt acatatgcgc cgcgattaac cgttggttat
    14221 cctggtacat tgcgaacagg tagttattat aactcttccc tttggtcgag tagcccttca
    14281 gcttgtgaat catcgctgtg gcagtcactt cctgatccgc tttacgcagc tgcgcacgcg
    14341 atttacggaa cgaagagtaa gccgggtgcg tattcaggtt agtgacatag gcgctcaccg
    14401 attctttgac agaactaaac tgtgagtacc ctttcacttt acccggcgca ttggtacaac
    14461 gtcctttcat gcatttcatg ccgaacagat tgttattgtt gcgcgccagc ttcgacgttc
    14521 cccaaccgct ttctgctgca gccatcgtcg ccaccatact ggtagggata atgtctacgc
    14581 gttcaagcaa ggtattccac gggatttttc gcgtattacc agaccacttc accttgtagc
    14641 gtttggcgat gtctttcaga cgcgcacgct cagcaggtga ccattggccc tgatactgtt
    14701 ttgaaatcag ccagttacgt tctgcagtaa tggctgcatt ttggctggta atgtaaggca
    14761 ttacggtccg gagaaacgcc tttttccttg gtgttccgga agggtatttt cgcaaatcag
    14821 gaagtgaact actctttgca ctattgcgag aatactcttg tttactgctt acctgtttat
    14881 tactagcttt agttaagtgg gacttttgac tcgctgttgt tgtgtgcgtc ttcgctaaca
    14941 cctcactcga aaacaccaga gtgagtaaca taagaatcat cgccccatat cgtcgtatgg
    15001 gagtcaaaat catcaggtct cctggtcgga tttaatcatt ccaacacctt atatttttca
    15061 caaatttgag agttgaatct caaatcatat caaaaatagc tgtcaagagc accccaagga
    15121 atagtccaaa tctgaaacta tgtcacgtgt taacgattca gattggcgct aaatcgcaga
    15181 aaatgtgggg tttatcgcaa aattcagccg ttttttgcgc gagatcgctc acccttgctt
    15241 ctcatcctgt ggacttaccg ctcagggatg agttttgttt ggcttatcgc tggcaaactg
    15301 tctgaaatcg cagcaataag gactcatccg ccatgaaact cgccgcctgt tttctgacac
    15361 tccttcctgg cttcgccgtt gccgccagct ggacttctcc ggggttccct gcctttagtg
    15421 aacagggaac gggaacattt gtcagccacg cgcagttgcc caaaggtacg cgtccactaa
    15481 cgctaaattt tgaccaacag tgctggcagc ctgcggatgc gataaaactc aatcagatgc
    15541 tttccctgca accttgtagc aacacgcagc ctcaatggcg attgttcagg gacggcgaat
    15601 atacgctgca aatagacacc cgctccggca cgccaacatt gatgatttcc atccagaacg
    15661 ccgccgaacc ggtagcaagc caggtccgtg tatgcccgaa atgggatgga ttaccgctca
    15721 cgctggatgt cagcgccact ttcccggaag gagccgccgt acgggattat tacagccagc
    15781 aaattgcgat agtgaagaac ggtcaaataa cgttacaacc cgctgccacc agcaacggtt
    15841 tactcctgct ggaacgggca gaaactgacg cctctgcccc tttcgactgg cataacgcca
    15901 cggtttactt tgtgctgaca gatcgtttcg aaaacggcga tcccagtaat gaccagagtt
    15961 acggacgtca taaagacggt atggcggaaa ttggcacttt tcacggcggc gatttacgcg
    16021 gcctgaccaa caaactggat tacctccagc agttgggcgt taatgcttta tggataagcg
    16081 ccccatttga gcaaattcac ggctgggtcg gcggcggtac aaaaggcgat ttcccgcatt
    16141 atgcctacca cggttattac acacaggact ggacgaatct tgatgccaat atgggcaacg
    16201 aagccgatct acggacgctg gttgatagcg cacatcagcg cggtattcgt attctctttg
    16261 atgtcgtgat gaaccacacc ggctatgcca cgctggcgga tatgcaggag tatcagtttg
    16321 gcgcgttata tctttctggt gacgaagtga aaaaaacgct gggtgaacgc tggagcgact
    16381 ggaaacctgc cgccgagcaa acctggcata gctttaacga ttacattaat ttcagcgaca
    16441 aaacaggctg ggataaatgg tggggaaaaa actggatcag aacggatatc ggcgattacg
    16501 acaatcctgg attcgacgat ctcactatgt cgctggcctt tttgccggat atcaaaaccg
    16561 aatcaactac cgcttctggt ctgccggtgt tctataaaaa caaaacggat actcacgcta
    16621 aagtcatcga aggctttaca cctcgcgatt acttaaccca ctggttaagt cagtgggtcc
    16681 gcgactatgg gattgatggt tttcgggtcg ataccgccaa acatgttgag ttgcccgcct
    16741 ggcagcaact gaaaaccgaa gccagcgccg cgcttcgcga atggaaaaaa gctaaccccg
    16801 acaaagcatt agatgacaaa cctttctgga tgaccggtga agcctggggc cacggcgtga
    16861 tgcaaagtga ctactatcgc cacggcttcg atgcgatgat caatttcgat tatcaggagc
    16921 aggcggcgaa agctgtcgat tgtattgcgc agatggatac gacctggcag caaatggcgg
    16981 agaaattgca gggtttcaac gtgttgagct acctctcgtc gcatgatacc cgtctgttcc
    17041 gtgaaggggg cgacaaagca gcagagttat tactattagc gccaggcgcg gtacaaatct
    17101 tttatggcga tgaatcctcg cgtccgttcg gtcctacagg ttctgatccg ctgcaaggta
    17161 cacgttcgga tatgaactgg caggatgtta gcggtaaatc tgccgccaac gtcgcgcact
    17221 ggcagaaaat cagccagttc cgcgcccgcc atcccgcaat tggcgcgggc aaacaaacga
    17281 cactttcgct gaagcagggc tacggctttg ttcgtgagca tggcgacgat aaagtgctgg
    17341 tcatctgggc tgggcaacag tgacttttcc ggcttcccgt tcgtcagtac ctcgggaagc
    17401 cgccaaccag gataaaatgt cagccctaat cagcgttgca ggataaagca ccgctcactc
    17461 ttcaacagac cgatttgcac cccagcaaat gtagcgttat tgttaccttc cttgctacag
    17521 agttcgacag atatcccgct atgacattct ccctttttgg tgacaaattt acccgccact
    17581 ccggcattac gctgctgatg gaagatctga acgacggttt acgcacgcct ggcgcgatta
    17641 tgctcggcgg cggtaatccg gcgcagatcc cggaaatgca ggactacttc cagacgctac
    17701 tgaccgacat gctggaaagt ggcaaagcga ctgatgcact gtgcaattac gacggtccac
    17761 aggggaaaac ggagctactc acactgcttg ccggaatgct gcgcgagaag ttgggttggg
    17821 atatcgaagc acagaatatt gcactaacaa acggcagcca gagcgcgttt ttctacttat
    17881 ttaatctgtt tgccggacgc cgtgccgatg gtcgggtcaa aaaagtgctg ttcccgctgg
    17941 caccggaata cattggctac gctgacgccg gactggaaga agatctgttt gtctctgcgc
    18001 gtccgaatat tgaactgctg ccggaaggcc agtttaaata ccacgtcgat tttgagcatc
    18061 tgcatattgg cgaagaaacc gggatgattt gcgtctcccg gccgacgaat ccaacaggca
    18121 atgtgattac tgacgaagag ttgctgaagc ttgacgcgct ggcgaatcaa cacggcattc
    18181 cgctggtgat tgataacgct tatggcgtcc cgttcccggg tatcatcttc agtgaagcgc
    18241 gcccgctatg gaatccgaat atcgtgctgt gcatgagtct ttccaagctg gggctgcctg
    18301 gctcccgctg cggcattatc atcgccaatg aaaaaatcat cactgccatc accaatatga
    18361 acggcattat cagcctggca cctggcggta ttggtccggc gatgatgtgt gaaatgatta
    18421 agcgtaacga tctgctgcgc ctgtctgaaa cagtcatcaa accgttttac taccagcgtg
    18481 ttcaggaaac tatcgccatc attcgccgct atttaccgga agatcgctgc ctaattcata
    18541 aaccagaagg agccattttc ctctggctat ggtttaagga tttgcccatt acgaccgagc
    18601 aactctacca gcgcctgaaa gcacgcggcg tgctgatggt gccggggcac aacttcttcc
    18661 cgggactgga taaaccgtgg ccgcatacgc atcaatgtat gcgcatgaac tacgtaccag
    18721 agccggagaa aattgaggcg ggggtgaaga ttctggcgga agagatagaa agagcctggg
    18781 ctgaaagtca ctaaaaattg cctgatgcgc tacgcttatc aggcctacat gatttctgca
    18841 atatattgaa tttggaagaa tttgtaggcc ggataaggcg tttacgccgc atctggcata
    18901 aacaaagcgc actattccgg cgttgagaaa cgccggaaaa cgtttcaaac aggctgctgc
    18961 cgtagccgat gccgctgcac tttctcgaca tccatgcact gcaacgcctt tgtcgggcag
    19021 gcttcaacac aggccggccc cgtctcccga tgccagcaca gatcgcactt aatcgccctc
    19081 gcctttttgc gcgaagaaac gacctccatc gcaccaaacg ggcaagccag catacagctt
    19141 ttacagccaa tgcaacgtgt ttgttcaacg aaaatatgcc catgttcgcg gcttatcgcg
    19201 tcaacagggc agacattcgc gcacggtgca tcttcacact gatgacaggc tactgccgtg
    19261 gtccagctgt ggtctttaat gacacgaata cgggaaataa actcgtctgg tgacaacgca
    19321 gcgcaatcct gattctcctg atgcgacact gcgcaagcca cttcacaggt acggcaaccg
    19381 atacatttcg tcgcatccgc aataataaac cggttcatga tcactccttt gcactaaaac
    19441 agcagagtgc cagagagcgc gtccctactg ctttgatcca acgaaggaaa tgttaaagtt
    19501 ttggcacttg tccaaagcgt tatgtgatcg cgcccaggtc atccctgaca gtaaatccca
    19561 gttcattaga aatagcctgc gcggtttcac gcagtggttt caggagattt ttctctccca
    19621 cctgtttcag acgtgatgtc gaaagcgaaa tcgacacggc gtacggcacc cgcccatgaa
    19681 tatcaaacac cggaacagca atacaggaga cgccgagttc gttttcttct ctgtccatcg
    19741 ccgctccgct ttcacgaatg tgcgccagtt cgtcgaacat cgcgggcagc tcggtaatgg
    19801 tattgcgggt taacggctgg atctcatgct gatggttttc ccagtatgac ttcacgtagt
    19861 ccgggtgacc aaacgccatg tagatcttgc ccattgcgga acagtagagc ggcatatgct
    19921 ggccaatata ggcacgggtt cgcagcatcc cggttgtggg ttccagctta taaatcaaaa
    19981 tagcgtgatc gtcttcgcgg ctggagaagt taatggtttc accagtggcg atgttcagtg
    20041 cctcaagatg cggagcggcg atatgaatga tattcagcga agacagcgcc ttctgcccga
    20101 cggcaataaa tttggtagtc aggcggtaac tccctgcggc gggcgcggtg gtcacatagc
    20161 cgcaggactg taatccctgc aataagcgat ggacggtact cttatttaaa ccagccagct
    20221 ccgaaagatg cgccaacgga caaccgtttg gatagttgct caaaatctca atcagcatca
    20281 acccgcgaaa cagactctgg cttccggctg gacgctcttt ttcctgcgcc atctcgttct
    20341 ctttttttcc catcacttct ttccccattt tgtcgcgtcc tgatggtagc gcaaagtgtg
    20401 ccgtagttca cgatctcgac agataattta taaccacttg atttttatgt cttttgaaat
    20461 tcatcaatca gattgctttg ttaaaaagtg atcgatatat ttgaaatcaa gtttcgcata
    20521 ttgaaatttc aagccaaaaa agcgatcaaa aaaacaagga agcctgggat gaaagtgaca
    20581 tttgagcagt taaaagcagc ctttaatcgg gtcttaattt cacgcggcgt tgacagcgaa
    20641 acggctgacg cctgtgcaga gatgttcgcc cgcaccaccg aatccggcgt ttattctcac
    20701 ggcgttaatc gtttccctcg tttcattcaa caactggaaa acggcgatat cattcctgat
    20761 gcccaaccca aacgtataac cagcctcggc gcaattgaac agtgggatgc ccagcgttcg
    20821 atcggcaacc tgacggcgaa aaagatgatg gatcgcgcca ttgaactggc tgccgatcac
    20881 ggtattggtc tggtggcact tcgtaatgcc aaccactgga tgcgcggcgg cagctacggc
    20941 tggcaggcgg cggaaaaagg ctatattggc atttgctgga gcaactccat cgccgtaatg
    21001 ccgccgtggg gcgcaaaaga gtgtcgcatc ggcactaacc cgctgatcgt cgccattcct
    21061 tccacgccga tcaccatggt cgatatgtcg atgtcgatgt tctcttacgg catgttagaa
    21121 gttaaccgcc tggcgggccg tcagctcccg gtcgatggtg gctttgatga tgagggcaat
    21181 ttgaccaaag aacctggcgt tatcgagaag aatcgccgca ttttgccgat gggctactgg
    21241 aaaggttctg gcatgtcgat tgtgctggat atgatcgcta ctctcctttc cgacggcgca
    21301 tcggttgccg aagtcaccca ggacaacagc gacgaatacg gcatttcaca aatttttatt
    21361 gccattgaag tggacaagct tatcgacggt cccacccgcg atgccaagct gcaacgtatc
    21421 atggattacg ttactaccgc tgaacgcgct gacgaaaacc aggccatccg cttacccggc
    21481 cacgaattta ctaccctgct ggccgaaaac cgccgtaacg gtattaccgt tgatgacagc
    21541 gtgtgggcca aaatccaggc gttataagga gataagtcat gatttttgga catatcgcgc
    21601 agcccaatcc gtgccgtttg cccgccgcca ttgaaaaggc gctcgatttt ctgcgcgcta
    21661 ccgatttcaa cgccctggag ccgggcgttg tcgaaatcga cggcaaaaat atttatgcgc
    21721 aaattatcga tttaaccaca cgcgaagcgg tggaaaaccg tccggaagtg catcgtcggt
    21781 atatcgatat tcagtttctg gcgtggggtg aagagaaaat cggcattgct attgatacgg
    21841 gaaataataa agtcagcgaa tcactattag agcagcgcga tattattttt tatcatgaca
    21901 gtgaacatga atcatttata gaaatgatac cgggcagcta cgcaattttc tttccgcagg
    21961 atgttcaccg accaggttgt attctgcaaa cagcctctga gatacgaaaa attgtggtta
    22021 aagtcgcatt aacagcgctg aattaatcaa ataatcgtct acgccagaac gactgggtaa
    22081 tgttattgct ctaactatat taataattca ggttgctgca catgcaactt gaattatgac
    22141 agacatagcc tcaaggaata gctatgaaaa aaatacttga agcaatactg gcgattaatc
    22201 tcgccgtact ttcctgtatt gtgtttataa acattatttt aagatatggt tttcagacaa
    22261 gcattttatc tgtagatgaa ttgtcacgtt atttatttgt ctggttaaca tttattggcg
    22321 cgattgtggc ttttatggat aacgcccacg ttcaggtcac ctttctggtg gagaaactct
    22381 cccccgcatg gcaacgacga gtcgctctgg ttacccattc cttaatctta tttatttgtg
    22441 gcgcactggc ttggggcgca acgctaaaaa caattcagga ctggagcgat tattcaccga
    22501 tcctcggttt gcccatcggc ctgatgtatg ccgcttgtct gcccaccagc ctcgttatcg
    22561 cattctttga actgcgtcat ttatatcaac tcatcacgcg tagcaattct ctaacgtcgc
    22621 caccgcaagg agcttaatca tggctgtgct gatttttctg ggctgtctgt tgggtggtat
    22681 tgctatcggt ttgcctattg cctgggcact gttgttgtgc ggggcggcgt taatgttctg
    22741 gctggacatg tttgatgtcc agatcatggc acaaacgctg gtgaacggag ccgacagctt
    22801 ctccctgctg gcgattccgt tctttgtgct ggcgggtgaa atcatgaatg cgggcggctt
    22861 gtcaaaacgc attgttgacc tgccgatgaa actggtgggg cataaaccag gcgggctggg
    22921 ctacgtcggc gtgctggcgg cgatgattat ggcgagcctt tctggttctg ccgttgcgga
    22981 taccgccgcc gttgccgctc tactggtgcc gatgatgcgc agcgccaact accctgtcaa
    23041 ccgggcggcg gggctgattg cctctggcgg cattatcgcg ccaattattc ccccttccat
    23101 tccgtttatt atcttcggcg tttccagcgg attatccatc agtaagctgt ttatggcggg
    23161 cattgcccct ggcatgatga tgggcgcaac gctgatgctt acctggtggt ggcaggccag
    23221 tcgccttaat ttaccccgcc agcaaaaagc aacgatgcag gaaatctggc actcttttgt
    23281 ctccggtatc tgggcgctgt ttcttccagt catcattatt ggcgggtttc gctccggtct
    23341 gtttacccct accgaagccg gggccgttgc tgctttctat gcgctgtttg tcgccacagt
    23401 tatttaccgt gaaatgacct tcgccacact ttggcatgta ctcattggcg cggcgaaaac
    23461 cacctcagtg gttatgtttc tggtggcctc ggcacaagtt tccgcctggc tgattaccat
    23521 tgctgaactg ccgatgatgg tttctgattt actgcaaccg ttggtcgatt caccgcgtct
    23581 gctgtttatc gtcattatgg tggctatcct gattgtcggc atggtcatgg atttaacgcc
    23641 gacggtatta attcttaccc cggtattaat gcctttagtt aaagaggcag gaatcgatcc
    23701 gatttatttc ggtgtcatgt ttatcattaa ctgctcaatc ggcttaatta caccgcctat
    23761 cggtaatgta cttaacgtta tttcaggggt ggcaaaactc aaattcgatg atgcagtcag
    23821 aggcgttttc ccttacgtcc tggttttata ttcattatta gtcgtatttg tttttattcc
    23881 cgatctcatc atcctccctt tgaaatggat taattaaaag gaaaatatta tgaaattacg
    23941 ctctgtaacc tacgcattat tcattgctgg cctggctgca ttcagcacat cttctctggc
    24001 ggcgcaatct ttacgtttcg gttatgaaac ctcacaaacc gactcgcaac atattgcgac
    24061 gaaaaaattc aatgatttat tgccggagag aaccaaaggc gagctgaaat taaaattgtt
    24121 tccggatagc accctcggta acgcgcaggc gatgatcagc ggcgtacgtg gcggcaccat
    24181 cgatatggaa atgtccggct cgaataactt tgccgggtta tcaccagtga tgaacttgct
    24241 tgatgtccct ttcctgttcc gcgataccgc tcacgcgcat aaaacgctcg acggcaaagt
    24301 cggtgatgat ctgaaagcct cacttgaagg taaaggacta aaagtactgg cctactggga
    24361 aaacggctgg cgcgatgtca ccaactcgcg cgcaccggtt aaaacccccg ccgacctgaa
    24421 agggctgaaa attcgcacca acaatagccc gatgaatatc gccgcattca aagtctttgg
    24481 cactaacccg atcccgatgc cgtttgccga agtctatacc gggctggaaa cccgcactat
    24541 cgacgctcag gaacacccga tcaacgtcgt ctggtcagca aaatttttcg aagtgcagaa
    24601 gtacctttct ctgacgcacc acgcctattc cccgcttctg gtggtgatca acaaagcgaa
    24661 gtttgatggc ttaagctcgg agttccagca agcgctaatt tcatctgcgc aggaagcggg
    24721 taactaccag cgcaaactgg ttgccgaaga tcagcaaaaa atcatcgacg gcatgaaaga
    24781 agcgggcgtg gaagtcatca ccgatctcga ccgcaaagcc tttagcgacg cactgggtac
    24841 tcaggtccgc gacatgtttg taaaagatgt tccgcagggt gctgatctac tgaaagccgt
    24901 ggatgaggtg caataaacca tgacgcaata ctggctgggg ttagattgtg gcggtagctg
    24961 gctgaaagcc gggctgtatg accgcgaagg ccgggaggca ggcgtgcagc gcctgccgct
    25021 gtgcgcatta agcccgcagc caggttgggc agagcgcgat atggcagaac tgtggcaatg
    25081 ctgcatggct gtcattcgcg ccctgcttac ccattccggc gttagcgggg agcaaatagt
    25141 gggtatcggt atctccgcac aaggaaaagg cttgtttttg ctggataaaa acaacaagcc
    25201 gctcggtaac gccattttgt cctcggatcg ccgggcgatg gaaatcgttc gtcgctggca
    25261 ggaagatggc atcccggaaa aactctaccc gctgacccga caaaccttgt ggaccgggca
    25321 tccggtgtcg ctgttacgtt ggcttaaaga gcacgaacca gaacgctacg cgcaaattgg
    25381 ctgcgtgatg atgacgcacg actacctgcg ctggtgttta accggcgtca aaggctgtga
    25441 agagagcaat atttccgagt ccaacctcta caacatgagt cttggggaat atgacccgtg
    25501 cctcaccgac tggctgggga ttgctgaaat caatcacgcc ctgccgcctg ttgtcggatc
    25561 tgccgaaatc tgcggggaga tcaccgctca gacagccgta ctgaccggtc tgaaagcggg
    25621 tacgcccgtt gtcggcggcc tgtttgatgt ggtttccacc gcactctgcg ccgggatcga
    25681 agacgaattt acccttaatg cggtgatggg cacctgggcg gtgaccagcg gcataacccg
    25741 cggtttacgt gacggtgaag cgcatccgta tgtctatggt cgctacgtta acgatggtga
    25801 atttatcgtt cacgaagcca gccccacctc ttccggcaac ctcgaatggt ttaccgcaca
    25861 gtggggagaa atctcgtttg atgagatcaa ccaggccgtt gccagcttgc cgaaggccgg
    25921 tggcgatctc tttttcctgc cgttcctgta cggcagcaac gccggactcg agatgaccag
    25981 cggtttctac gggatgcagg ccattcacac ccgcgcacac ctgttgcagg ccatttatga
    26041 aggggtggtg ttcagccata tgacccatct caaccggatg cgcgaacgtt ttactgatgt
    26101 gcacaccctg cgcgtcactg gcggcccggc gcattccgat gtctggatgc aaatgctggc
    26161 ggacgtcagc ggtctgcgta ttgagctgcc gcaggtggaa gaaaccggct gctttggtgc
    26221 agcacttgcc gcccgcgtcg gcaccggggt ttatcgcgat ttcagcgaag cccagcgtga
    26281 tttacagcac ccggtgcgca ccctgctgcc ggatatgact gtccatcagc tttaccaaca
    26341 aaaataccaa cgctatcagc atctcattgc cgcacttcag ggctttcacg cccgcatcaa
    26401 ggagcacata ttatgagccg accacttctg caactggcac tcgaccactc atcacttgaa
    26461 gccgcacagc gcgacgtgac gcagttaaaa gacagcgtcg atattgtcga agcgggcacc
    26521 attctctgtt taaacgaagg gcttggcgcg gtgaaagcct tgcgcgaaca gtgccctgac
    26581 aaaatcatcg ttgccgactg gaaggtcgcc gacgctggtg aaacgctcgc gcaacaggca
    26641 tttggcgcag gcgctaactg gatgaccatc atctgcgccg caccgctcgc gacggtggaa
    26701 aaaggccacg cgatggcgca acgctgcggt ggtgaaattc agatcgagct gttcggtaac
    26761 tggacgctgg acgacgcccg cgactggcat cgtattggcg tgcggcaggc gatttatcat
    26821 cgcggtcgtg atgcacaggc cagcgggcaa cagtggggcg aagccgatct ggcacgcatg
    26881 aaggcgcttt ccgatatcgg ccttgagctt tccattaccg gcgggattac ccctgctgac
    26941 ctgccgctgt ttaaagatat tcgcgtgaaa gcgtttattg ccgggcgagc actggcaggc
    27001 tcagcaaatc cggcgcaggt ggctggcgat ttccatgcgc aaatcgacgc tatctgggga
    27061 ggcaagcgtg cgtaatcatc cgttagggat ttatgaaaag gcgctggcga aagatctctc
    27121 ctggccggag cggctggtgc tggcaaaaag ctgcggtttt gattttgtcg aaatgtcggt
    27181 ggatgaaacc gacgaacggc tttcacgtct tgactggagc accgcacaaa ggacttcgct
    27241 ggttgccgcg atgatcgaaa cgggtgttgg gatcccgtcg atgtgcctgt ccgcacatcg
    27301 tcgctttcct tttggtagcc gtgacgacgc ggtacgccag cgagcacggg agatcatgag
    27361 caaagcgatt cgtctggcac gcgatcttgg catacgcacc atccagctgg cgggctacga
    27421 cgtctattac gaagaccacg atgaaggcac ccggcaacgt tttgctgaag ggctggcgtg
    27481 ggcggtcgaa caggcggcgg catcacaagt catgctagcg gtggagatta tggacaccgc
    27541 gtttatgaac tccatcagta aatggaaaaa gtgggacgag atgctcgcct cgccgtggtt
    27601 caccgtctac ccggacgtcg gcaacctcag cgcctggggc aatgatgttc ccgccgaact
    27661 gaaactgggc attgaccgta tcgccgcgat ccacctgaaa gacacccagc cagttaccga
    27721 gcaaagtccc ggacagttcc gcgacgtgcc gtttggcgaa ggctgcgtcg atttcgttgg
    27781 catcttcaaa acgctgcata aactgaacta tcgcggttct ttcctgattg agatgtggac
    27841 cgaaaaagcc aaagagccgg tgctggagat tattcaggcg cggcgttgga ttgaagcgcg
    27901 tatgcaggag gctggattta tatgttagag caactgaaag ccgacgtgct ggcggcgaat
    27961 ctggcgcttc ccgctcacca tctggtgacg ttcacctggg gcaatgtcag cgcggtagac
    28021 gaaacgcggc aatggatggt aatcaaacct tccggcgtcg agtatgacgt gatgaccgcc
    28081 gacgatatgg tggtggttga gatagccagc ggcaaggtgg tggaaggtag caaaaagccc
    28141 tcgtccgaca cgccaacgca tctggcgctt taccgtcgct acgccgaaat cggtggcatt
    28201 gtgcataccc actcacgcca cgccaccatc tggtcgcagg ccgggctgga tccccccgcc
    28261 tggggcacca cgcacgctga ttatttctat ggtgccatcc cctgcacgcg acagatgacc
    28321 gcagaggaga ttaacggcga atatgaatat cagaccggcg aagtgatcat tgaaaccttc
    28381 gaagaacgtg gcaggagtcc ggcacaaatc ccggcggtgc tggtgcattc tcacggcccg
    28441 ttcgcatggg gtaaaaacgc cgccgatgcc gtgcataacg ccgtagtact cgaagaatgc
    28501 gcctatatgg gcctattctc gcgccagctt gcgccgcagc tccctgcgat gcaaaacgaa
    28561 ctgctggata agcactacct gcgtaagcat ggggccaatg cctattacgg gcagtaatcc
    28621 ctcacgccgg ggcttcatcg ccccggcact acgaattgat atgttccttg ctgtaacgcc
    28681 gcttccacgc tgctggcgtt aaaccggtgt gcttgcgaaa aatctgccgg aaatagccaa
    28741 cgtcattaaa ccgtattgca tggagcaggc cgacaacgtc gagtcactgc ataacctgtt
    28801 gctgcccgcc gatgcgccat ttacgacatt tgaaggcaag ggattgttca gccataagat
    28861 cctgattcag acgccaggtt cccggtatga acagagcaat ccagagcaac aacctttgtg
    28921 gcattacgac ggagcaccag ccgcaccgac atccaccggt gaattaaaaa cgccagctca
    28981 cgccggtgac aaaggtaaaa ctctcattac gatcaatcat cgggctgtcg gtaatctctt
    29041 ccggcagctc gctgtatcct gcgctcgcca tcaatacgac gtgctctcct atcgggtatt
    29101 ttgccgtcag gctgacatag ggcacccagg catcctgcgc ggaataactt gccagaccgc
    29161 tacgacggga ctcactctct gaaatgccat agtaatagtc actgaaattc tcgtcataat
    29221 agagaacgcc cagcgcaggt gtcagcgaca ggcgacctat ctgcattttg tggaataccg
    29281 atagctcccc cacccagccg ttgctgttat ccagaacgtc cgcagccgca gaggctttca
    29341 cggttcccca acgctcgtgg tgataccagg caacccccgc catagccgta ctatcacgct
    29401 tatcaagctg ttgcatggca taatcgtcat tatcggcagg gtcaaattcc agcggcatcc
    29461 aggatgcggt caggctaagt tcattttttt cactttgcga cagaataaaa cctaatgtgg
    29521 tctgacgaac ataaaaagaa tcaccttcat aactaatcag cggaattgcc tttgtatttt
    29581 cattataacc gcgataaggc gattcattat aagctgcacc agcaccaatg gataattcag
    29641 aagcagttgc gcttgccata aaaggcaacg caaataacgc cacaatattg cgattaatta
    29701 acatgtccgg tattccattt t
//
LOCUS       DAZKHC020000053        29554 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZKHC020000053 DAZKHC020000000
VERSION     DAZKHC020000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.053"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(129..1133)
                     /gene="argF"
                     /locus_tag="ACHOSM_RS13605"
                     /old_locus_tag="pgaptmp_002721"
     CDS             complement(129..1133)
                     /gene="argF"
                     /locus_tag="ACHOSM_RS13605"
                     /old_locus_tag="pgaptmp_002721"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGU4559214.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1295..1711
                     /gene="rraB"
                     /locus_tag="ACHOSM_RS13610"
                     /old_locus_tag="pgaptmp_002722"
     CDS             1295..1711
                     /gene="rraB"
                     /locus_tag="ACHOSM_RS13610"
                     /old_locus_tag="pgaptmp_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HGU4559215.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1757..2260)
                     /gene="yjgM"
                     /locus_tag="ACHOSM_RS13615"
                     /old_locus_tag="pgaptmp_002723"
     CDS             complement(1757..2260)
                     /gene="yjgM"
                     /locus_tag="ACHOSM_RS13615"
                     /old_locus_tag="pgaptmp_002723"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4559216.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            2453..3649
                     /gene="yjgN"
                     /locus_tag="ACHOSM_RS13620"
                     /old_locus_tag="pgaptmp_002724"
     CDS             2453..3649
                     /gene="yjgN"
                     /locus_tag="ACHOSM_RS13620"
                     /old_locus_tag="pgaptmp_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HGU4559217.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRHQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAVGMGTV
                     FFISTKMLHANSSSSVIISMVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLAGISRGVM
                     PSLPFL"
     gene            complement(3705..6560)
                     /gene="valS"
                     /locus_tag="ACHOSM_RS13625"
                     /old_locus_tag="pgaptmp_002725"
     CDS             complement(3705..6560)
                     /gene="valS"
                     /locus_tag="ACHOSM_RS13625"
                     /old_locus_tag="pgaptmp_002725"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGU4559218.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(6560..7003)
                     /gene="holC"
                     /locus_tag="ACHOSM_RS13630"
                     /old_locus_tag="pgaptmp_002726"
     CDS             complement(6560..7003)
                     /gene="holC"
                     /locus_tag="ACHOSM_RS13630"
                     /old_locus_tag="pgaptmp_002726"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HGU4559219.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(7137..8648)
                     /gene="pepA"
                     /locus_tag="ACHOSM_RS13635"
                     /old_locus_tag="pgaptmp_002727"
     CDS             complement(7137..8648)
                     /gene="pepA"
                     /locus_tag="ACHOSM_RS13635"
                     /old_locus_tag="pgaptmp_002727"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HGU4559220.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8802..8852
                     /gene="ytgA"
                     /locus_tag="ACHOSM_RS13640"
                     /old_locus_tag="pgaptmp_002728"
     CDS             8802..8852
                     /gene="ytgA"
                     /locus_tag="ACHOSM_RS13640"
                     /old_locus_tag="pgaptmp_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HGU4559221.1"
                     /translation="MSIIILATISTTQENK"
     gene            8915..10015
                     /gene="lptF"
                     /locus_tag="ACHOSM_RS13645"
                     /old_locus_tag="pgaptmp_002729"
     CDS             8915..10015
                     /gene="lptF"
                     /locus_tag="ACHOSM_RS13645"
                     /old_locus_tag="pgaptmp_002729"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGU4559222.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            10015..11097
                     /gene="lptG"
                     /locus_tag="ACHOSM_RS13650"
                     /old_locus_tag="pgaptmp_002730"
     CDS             10015..11097
                     /gene="lptG"
                     /locus_tag="ACHOSM_RS13650"
                     /old_locus_tag="pgaptmp_002730"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGU4559223.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(11228..12730)
                     /gene="yjgR"
                     /locus_tag="ACHOSM_RS13655"
                     /old_locus_tag="pgaptmp_002731"
     CDS             complement(11228..12730)
                     /gene="yjgR"
                     /locus_tag="ACHOSM_RS13655"
                     /old_locus_tag="pgaptmp_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HGU4559224.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRTFTPKDQKAV
                     KAAAQTMRVNPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(12859..13878)
                     /gene="ahr"
                     /locus_tag="ACHOSM_RS13660"
                     /old_locus_tag="pgaptmp_002732"
     CDS             complement(12859..13878)
                     /gene="ahr"
                     /locus_tag="ACHOSM_RS13660"
                     /old_locus_tag="pgaptmp_002732"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HGU4559225.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            14074..14158
                     /locus_tag="ACHOSM_RS13665"
                     /old_locus_tag="pgaptmp_002733"
     tRNA            14074..14158
                     /locus_tag="ACHOSM_RS13665"
                     /old_locus_tag="pgaptmp_002733"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14108..14110,aa:Leu,seq:caa)
     gene            14357..15542
                     /locus_tag="ACHOSM_RS13670"
                     /old_locus_tag="pgaptmp_002734"
                     /pseudo
     CDS             14357..15542
                     /locus_tag="ACHOSM_RS13670"
                     /old_locus_tag="pgaptmp_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            15576..16772
                     /locus_tag="ACHOSM_RS13675"
                     /old_locus_tag="pgaptmp_002735"
                     /pseudo
     CDS             15576..16772
                     /locus_tag="ACHOSM_RS13675"
                     /old_locus_tag="pgaptmp_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc52 family transposase"
     gene            16855..17112
                     /locus_tag="ACHOSM_RS13680"
                     /old_locus_tag="pgaptmp_002736"
     CDS             16855..17112
                     /locus_tag="ACHOSM_RS13680"
                     /old_locus_tag="pgaptmp_002736"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559226.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(17670..18437)
                     /gene="fecE"
                     /locus_tag="ACHOSM_RS13685"
                     /old_locus_tag="pgaptmp_002737"
     CDS             complement(17670..18437)
                     /gene="fecE"
                     /locus_tag="ACHOSM_RS13685"
                     /old_locus_tag="pgaptmp_002737"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HGU4559227.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWQSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(18438..19394)
                     /gene="fecD"
                     /locus_tag="ACHOSM_RS13690"
                     /old_locus_tag="pgaptmp_002738"
     CDS             complement(18438..19394)
                     /gene="fecD"
                     /locus_tag="ACHOSM_RS13690"
                     /old_locus_tag="pgaptmp_002738"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HGU4559228.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(19391..20389)
                     /gene="fecC"
                     /locus_tag="ACHOSM_RS13695"
                     /old_locus_tag="pgaptmp_002739"
     CDS             complement(19391..20389)
                     /gene="fecC"
                     /locus_tag="ACHOSM_RS13695"
                     /old_locus_tag="pgaptmp_002739"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HGU4559229.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTSIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(20386..21288)
                     /gene="fecB"
                     /locus_tag="ACHOSM_RS13700"
                     /old_locus_tag="pgaptmp_002740"
     CDS             complement(20386..21288)
                     /gene="fecB"
                     /locus_tag="ACHOSM_RS13700"
                     /old_locus_tag="pgaptmp_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HGU4559230.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASIDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(21333..23657)
                     /gene="fecA"
                     /locus_tag="ACHOSM_RS13705"
                     /old_locus_tag="pgaptmp_002741"
     CDS             complement(21333..23657)
                     /gene="fecA"
                     /locus_tag="ACHOSM_RS13705"
                     /old_locus_tag="pgaptmp_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HGU4559231.1"
                     /translation="MTPLRVFRKTTPLVNTIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGLLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(23744..24697)
                     /gene="fecR"
                     /locus_tag="ACHOSM_RS13710"
                     /old_locus_tag="pgaptmp_002742"
     CDS             complement(23744..24697)
                     /gene="fecR"
                     /locus_tag="ACHOSM_RS13710"
                     /old_locus_tag="pgaptmp_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HGU4559232.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(24694..25215)
                     /gene="fecI"
                     /locus_tag="ACHOSM_RS13715"
                     /old_locus_tag="pgaptmp_002743"
     CDS             complement(24694..25215)
                     /gene="fecI"
                     /locus_tag="ACHOSM_RS13715"
                     /old_locus_tag="pgaptmp_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HGU4559233.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            25352..25420
                     /gene="ykiE"
                     /locus_tag="ACHOSM_RS13720"
                     /old_locus_tag="pgaptmp_002744"
     CDS             25352..25420
                     /gene="ykiE"
                     /locus_tag="ACHOSM_RS13720"
                     /old_locus_tag="pgaptmp_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HGU4559234.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            25507..26204
                     /locus_tag="ACHOSM_RS13725"
                     /old_locus_tag="pgaptmp_002745"
                     /pseudo
     CDS             25507..26204
                     /locus_tag="ACHOSM_RS13725"
                     /old_locus_tag="pgaptmp_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
     gene            complement(26215..26286)
                     /locus_tag="ACHOSM_RS13730"
                     /old_locus_tag="pgaptmp_002746"
                     /pseudo
     CDS             complement(26215..26286)
                     /locus_tag="ACHOSM_RS13730"
                     /old_locus_tag="pgaptmp_002746"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26346..26495
                     /locus_tag="ACHOSM_RS13735"
                     /old_locus_tag="pgaptmp_002747"
                     /pseudo
     CDS             26346..26495
                     /locus_tag="ACHOSM_RS13735"
                     /old_locus_tag="pgaptmp_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            26522..26626
                     /locus_tag="ACHOSM_RS13740"
                     /old_locus_tag="pgaptmp_002748"
                     /pseudo
     CDS             26522..26626
                     /locus_tag="ACHOSM_RS13740"
                     /old_locus_tag="pgaptmp_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            26941..27222
                     /locus_tag="ACHOSM_RS13745"
                     /old_locus_tag="pgaptmp_002749"
     CDS             26941..27222
                     /locus_tag="ACHOSM_RS13745"
                     /old_locus_tag="pgaptmp_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HGU4559235.1"
                     /translation="MTEPWGRKMHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAV
                     NALFCEREIINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(27560..27691)
                     /locus_tag="ACHOSM_RS13750"
                     /old_locus_tag="pgaptmp_002750"
                     /pseudo
     CDS             complement(27560..27691)
                     /locus_tag="ACHOSM_RS13750"
                     /old_locus_tag="pgaptmp_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            27973..29331
                     /locus_tag="ACHOSM_RS13755"
                     /old_locus_tag="pgaptmp_002751"
     CDS             27973..29331
                     /locus_tag="ACHOSM_RS13755"
                     /old_locus_tag="pgaptmp_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HGU4559236.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
BASE COUNT         6938 a         7675 c         7799 g         7142 t
ORIGIN      
        1 ttaaatcctc cggaagattt acgtatagca ataaaaaaat tggggtggaa taattgaact
       61 atcactgcat aaaataatgt gacgccggga tgtttttgta tttcccggca tctttatagc
      121 gatatcaatt atttactgag cgtcgcgacc atcaccgctt tgatggtatg catgcggttt
      181 tccgcctgat caaatacaat gctggcggca gattcgaaga cctcatcagt cacttccatt
      241 ccgccatgta ggccaaactc ttccgccatt tttttgccaa gcgtcgtttg gtcgtcatga
      301 aacgcgggca ggcagtggag gaatttgacc tccgggttac cggtcaactg catcatcttg
      361 ctgttcactt gataatcacg cagcaatgca atgcgttcag cccatttctc ttttgcttca
      421 cccatcgaca cccatacatc ggtatagata aagtcagcac cttcaactcc cttggctaca
      481 tcttccgtta gcgtaatatt cccgccattt tgctgtgcca gagcgcggca ttccgtaacc
      541 agcgcagctt ccggccagca tgcctgtggc gcgaccagac gcaaatccag accggtaagc
      601 gccgcagctt cgagcatcga attgcccatg ttgttacgtg cgtcacctgc atagaccagc
      661 gtcatttcgt tgaatgcttt accgggtaaa tgctcctgca tggtgaggag atccgccagc
      721 aactgcgtgg ggtgaaactc attggtcagg ccgttccata ccggcacgcc agcgtattcc
      781 gccagtgttt cgacaatctc ctgaccatag ccgcgatact gaataccgtc atacatgcgg
      841 cccagcacgc gggcagtgtc tttaatcgac tctttatgac caatctgact gccgcttggg
      901 ccgagataag taacgcgagc accctggtca tatgcggcaa cttcgaaaga gcatcgggta
      961 cgggtcgagt ctttttcgaa gatgagcgcg atgtttttac cagtgagttt ggcttcttct
     1021 ttgccgcttt tcttatcggc tttcagcttc gcggctaact gcagcaggct gttgagttca
     1081 gctggcgtga aatcgagtaa tttcaggaaa tgcttatgat aaaacccgga catagatccc
     1141 tcctgtggct aacgcctcaa tgaattaaaa ttcaatttat atggatgatt attcatttgc
     1201 aagtctaaag cataaatctt tgtcacaaag gtggaggcaa tgtcagtggt gtgtgacaat
     1261 aagagtatcg gcaggacatt aagaggaatg agccatggca aacccggaac aactggaaga
     1321 acagcgtgaa gaaacacgtt tgattattga agaattactg gaagatggca gcgatccgga
     1381 cgcactgtat accatcgaac accatctttc cgcagacgat ctggaaaccc tggaaaaagc
     1441 agcagttgaa gcgtttaaac tcggttacga agtgaccgat ccagaagagc tggaagttga
     1501 agatggtgat atcgtgattt gctgcgacat cctcagcgag tgcgcattga atgccgatct
     1561 gatcgatgcc caggttgaac aactgatgac gctggcggag aaatttgacg ttgagtacga
     1621 cggttggggc acttactttg aagatcctaa cggcgaagat ggcgacgatg aagattttgt
     1681 cgatgaagac gatgacgggg ttcgccacta attaattgat taaaatgccc gatagcacat
     1741 actagcgggc atttttttag agttcgcgta gcatccgcac ttcacaatcg acatggcccg
     1801 tgcagccaag cgcatagtcg atatgctcaa agcccaaatg ctcataaagc gcaatggctt
     1861 cctttaaaaa agcggtcgtt tccagatagc agcgtttgaa gcccatctct cgtgcctgct
     1921 ccatcgccat taaggccagt tttttcgcta gccctttacc gcgaatagcc ggaagaaaat
     1981 acatcttttg cagttcgcaa atgtccgact cactcccggt taatggcgca atcccaccgc
     2041 cgccgaccac ttcaccctcg tactcaacca cccaatatgc atggccaggc tgactataca
     2101 cttgatacag ctcgtcaaga ttcggatcgg cgacggtgta gcctttatca gcggtaagac
     2161 cgtattcggc ggatacctga cgaatgacgc gggcgatagc aggattatcc tgcagcgtca
     2221 ggcgacgcat tacaggtgat tgcggcgcaa tgttattcat agtttaaccc aataaaaata
     2281 aagtaattat acatttgttt aataccactc tcttcttacg tgcgcaagcg tcttataaag
     2341 caataaagtt tttgccagcc tattactgat aagagtattg taacgtggca gaacatttta
     2401 ctgtctgtat ctataaatta tatcaatgga ttttttaatt cagggaattt ttatggctca
     2461 agttattaat gaaatggatg tgccgtccca ttcgtttgtt tttcatggta caggtgagag
     2521 atattttctt atttgtgtgg tgaatgtgtt gttaacgatt ataacgctag gtatctattt
     2581 accatgggca ttaatgaaat gtaagcgtta tctttatgct aatatggaag ttaacggaca
     2641 acgattttct tatggaatta ccggtgggaa tgtttttgtt agttgtcttg tttttgtttt
     2701 tttctatttc gcaatcttaa tgacagtgtc agcagatatg ccgcttgttg gttgtgtttt
     2761 gactttgtta ctgttggttt tgcttatatt tatggcagca aaaggactgc gtcatcaggc
     2821 cttaatgacc agtctcaacg gcgtaagatt tagttttaat tgctctatga aagggttctg
     2881 gtgggtgacc tttttcttgc cgattttaat ggccgttggg atggggactg ttttctttat
     2941 ctcgacaaag atgctacatg ccaatagttc aagtagtgtt attatatcta tggttctgat
     3001 ggcaatagtt ggtattgttt ccattggtat ttttaatggt actttatata gtctggtaat
     3061 gagttttctc tggagcaata ccagtttcgg tatacatcgt ttcaaggtga aattagatac
     3121 tacgtattgt ataaaatatg ccattctcgc atttttagct ttattgcctt ttctcgctgt
     3181 tgctggttat attatcttcg atcaaatatt aaatgcgtat gatagttctg tatatgcaaa
     3241 tgatgacatt gagaatttac agcaatttat ggaaatgcaa cgtaaaatga taatcgcgca
     3301 gttaatctat tattttggga ttgctgttag caccagttat ttaacggtgt cgttgcgaaa
     3361 tcattttatg agcaacctgt cactgaatga tgggcgtatt cgttttcgct caactttaac
     3421 gtaccacggt atgctttatc gcatgtgtgc gttggtggtg atatccggga ttacgggcgg
     3481 tctggcttat ccactgttga aaatatggat gattgactgg caggcaaaaa atacgtattt
     3541 gctgggcgat ttggatgacc ttcctttaat caataaagaa gaacaaccag ataaaggctt
     3601 cttagccggt atttctcggg gagttatgcc ttctttacca tttctgtaag agataaaaaa
     3661 ggccggagca tgctccggcc ttcgttttca tcactgtgtt ttgattacag cgcggcgata
     3721 acagcctgct gttcaatcag cttcgctttc gcttccgcat agccttccag cttctcacgc
     3781 tctttcgcga tgaccgcttc cggtgcgcgg gcgacaaagc cttcattcgc cagcttgttc
     3841 tcgatacggc tgatttcgcc ttcgatcttc gccacttctt tcgccagacg cgccagctca
     3901 tcttctttgt tgatgaggcc agccatcggg atcagcagct ctgcgccgtc gatgatcttc
     3961 gtaacggaaa ccggaccttt gtcatcggca ggcagcacgg tgatgctttc cagacgcgcc
     4021 agggtttgca ggaagccacg gttttcattt acgcgacgtt ctgcatccgc gctgcaacca
     4081 cgcagcagca gttccagcgg tttgcccggc gcgatgttca tttctgcacg gatgttacgt
     4141 acagcaacga tcgcctgttt cagccactcg gtgtcggcca gtgcggcttc atccacctga
     4201 gacgcatcgt actgcgggaa cggttgcagc atgatggtgt cggcagtgat accgcaaagt
     4261 actttcacac gctgccagat ggtttcggtg atgaatggaa tgatcggatg cgcgaggcgc
     4321 agcagtcctt ccagtacagt caccagcgta tggcgagtac cgcgcagttc ggcttctgtt
     4381 ccaccgttca ttaccggctt ggtcagctcc agataccagt cacagaactg gttccaggtg
     4441 aactcataca aaatgcctgc ggcgatatcg aagcggaagc tgtccagcgc ttcgcggtac
     4501 gctttgatgg tctggttgaa ctccgccaga atccagcggt ccgccagcga cagcgtcatt
     4561 tcaccgccgt tgaagccgca atcctgacct tctgtgttca tcagcacaaa gcggctggcg
     4621 ttccacagct tgttacagaa gttacggtag ccttccagac gcttcatatc ccagttgatg
     4681 tcacgaccgg tagaagccag cgccgccagg gtgaagcgca gcgcgtcagt accgtgcggc
     4741 tcaatgccgt tcgggaactg cttctcggtg cgcttacgga ttttgtccgc cagctgcggc
     4801 tgcatcatat tgccggtacg tttctccagc agttccggca gcgaaatacc gtcaaccata
     4861 tccagcgggt cgataacgtt acccttggat ttggacatct tctggccttc gtcgtcacga
     4921 atcaggccgg tcatgtaaac ggtgtggaac ggcacctgcg gtttgccatt ttcatctttg
     4981 atgaagtgca tggtcatcat gatcatgcgg gcaatccaga agaagatgat gtcgaaacca
     5041 gataccatca cgctggttgg gtggaactga cgcagggcgt cggtgttttc cggccagcca
     5101 agggtagaga aggtccacag cgcagaagag aaccaggtgt cgagaacgtc ttcgtcctga
     5161 cgcagggcaa cgtcggcacc gaggttattt tctttacgca cttcgtcttc gttgcggcca
     5221 acataaacgt tacccgcttc gtcgtaccat gccgggatac ggtgacccca ccacagctga
     5281 cgagagatac accagtcctg aatatcgcgc atccaggaga agtacatgtt ttcgtactgc
     5341 ttcggtacga actgaatgtc gccgttctca accgcttcaa ctgccggctt cgccaggaca
     5401 tcggcacgca cgtaccactg gtcggtcagc atcggttcga taactacgcc gccacggtcg
     5461 ccgtaaggaa cggtcaggtc gtgcggttta atttcttcca gcaggccgag tgcgtcaacc
     5521 gcagcaacga ctgctttacg tgcggcaaaa cgctccagtt tctggaactc tgccgggatt
     5581 tcgctggaat aaacgtcaga ttcgttacct ttggtatcga acacctgggc gctttcacgg
     5641 atatcaccgt caaaggtcag gatgttgatc atcggcagcg cgtgacgttt accgacttca
     5701 tagtcgttaa agtcgtgcgc cggagtgatt ttcacgcagc cggtgccttt ttccatgtcg
     5761 gcgtgttcgt cgccaacgat cggaatacga cggttaacca gcggcagaat gacatatttg
     5821 ccaatcagat ctttgtaacg cggatcttcc gggttaacag ctacaccggt atcgcccagc
     5881 agggtttccg ggcgagtggt cgcaaccacc agataatctt taccgtctgc ggttttcgcg
     5941 ccgtcagcca gcggatagcg gatgtgccac atcgaacctt tcgattcgcg attttccact
     6001 tccaggtcag agatagcggt acgcagtttc ggatcccagt ttaccaggcg tttgccacgg
     6061 taaatcaggt cttctttata cagacgaacg aaaacttctt tcaccgcatt ggacaggcct
     6121 tcgtccatgg tgaagcgttc acgctcccag tcgacggagt tgccgagacg gcgcatctga
     6181 cgggtaatcg tgccgccgga ttccgctttc cattcccaga ttttgtcgat gaaagcttcg
     6241 cggccgtagt cgtgacgggt tttaccttct tctgcggcaa tcttgcgctc aacgaccatc
     6301 tgggtagcga tcccggcgtg gtcagtaccg acctgccaca gggtgttttt gccctgcatg
     6361 cgctgatagc ggatcatggt atccatgatg gtttgctgga aggcgtgacc catatgcaaa
     6421 ctgccggtga cgttcggcgg cgggatcatg atgcagaaac tttcctggct ttcatcgcca
     6481 ttaggcttaa agtagccctg cttttcccag tgctcgtaaa gcggctgttc gatatcttgt
     6541 gggttatatg tcttttccat tatttccagg ttgccgtatt caggttgaaa ccagccacgc
     6601 ggtaggcttt atagcgttcg cgcgccagtt gtttcagaga atcttcataa ggaacgaagt
     6661 ctaccacttc tgtgaaagcg gtggcaaaat ctgcaaagct tgttcgcaga ctaatcaata
     6721 tatcgcgcgg gctgctgctg cgtttttgcg gccaggcgat ctccaccggc gcaccgccgc
     6781 gcggtccttc tcccgctaaa ttatgcggga caaagctttc tgccggacgc gcccacaggg
     6841 cttcatccag ccggtaagcc tgcttttcat cttcacaggc gatgagcacg cgcttaccgc
     6901 tgcgccaacg ttctgcggca atttcacaca ccagttgctc aacggcgctt aagccatcga
     6961 cggtggtgtc attgtccaga aggtagaacg ttgcgttttt catatatggg gcttcttgtg
     7021 gtggatttaa atattgctgc attcctcacc ctgaaagatg agcaatgaat ttctattgcg
     7081 ctcgatcggt tccctctccc ttagggagag ggttagggtg agggtaaaac gcaaacttac
     7141 tcttcgccgt taaacccagc gcggtttaac aggaactgtg ccagcaacgc taccggacga
     7201 ccggttgcgc cttttgcttt accagaacgc caggcggttc ctgcaatatc caggtgcgcc
     7261 cagttgtact tacgggtaaa gcgtgacagg aagcaacctg cggtaatcgc cccaccagga
     7321 cgaccgccaa tgttcgccat atcggcaaaa ttggactcca gttgttcctg atactcgtca
     7381 cccagcggta agcgccatgc gcggtcaccg gattgttcag acgcggcaat cagttcatgg
     7441 gccagcggat tgtggttcgc catcagacca gtaatatgat gacccagcgc gatcacgcag
     7501 gcaccggtca gcgtcgccac atcaatcacc gcttccggct caaaacgttc aacgtaagtt
     7561 aacacgtcgc acagtaccag gcggccttcg gcatcggtgt tcagcacttc aacggtttga
     7621 ccggacatgg tggttaacac atcgcccgga cgataggcac gcccgccagg catgttttcg
     7681 cagcctgcca gcacgccgat aacgttaatc ggcagttgca gctccgcgac catacgcatc
     7741 acgccgtaaa ccgccgccgc gccgcacata tcgtacttca tctcatccat gccttctgaa
     7801 ggcttgatgg agataccgcc ggagtcgaag gttaaacctt tacccaccag cacgattggg
     7861 cgagcatctt ccgacgcgtt gcctttgtac tcaatcaccg acatcagcga ttcgttctgc
     7921 gaaccctgac cgaccgccag ataagaatgc atccccagct ctttcatctg ctgttcgccg
     7981 ataacgcggg tgatgacatt cttgctgtag ctgtcagcca gctggcgcgc ctgagaggcg
     8041 agataagcgg cgttacagat atttggcggc atattaccga gatctttcgc ggcttttatc
     8101 ccggcggcaa tcgccagacc gtgctggatc gcgcgctcac cgctggtcag ttcacggcgg
     8161 gtcggcacgt tgaacaccat tttacgcagc ggacgacgcg gttcgctctt gttcgttttc
     8221 agctgatcga aactgtagag cgtctctttt gcagtctcga cagcctgacg cactttccag
     8281 tagttgttac ggcctttaac gtgcagttca gtcagaaagc agaccgcttc catcgagcca
     8341 gtatcattca gcgtattaat ggttttctga ataacctgct tgtactgacg ctcatccagc
     8401 tcacgttctt tgccgcaacc aataaggaga attcgctcgg aaagtacatt cggaacatgg
     8461 tgcagcaaca gtgtctgccc cggttttcct tccagttcgc cccgacgtag cagggcgctg
     8521 atgtacccat cgctgatttt atcgagctgt tctgcaatcg gagaaaggcg acgtggttcg
     8581 aagacgccca cgacgatgca ggcactccgc tgtttctccg ggctaccgct ttttacacta
     8641 aactccatgc actacgctcc tgaatcttaa agacaacggc ggtggctaca gatagaattg
     8701 caagctttcg taactcatgt ccgctgttgc gatgacttcg tgttaatctt aacgttatta
     8761 cggcattggc acgtcagaac aaaatctgag aggtgaatcc gttgagtata attatcttag
     8821 cgacgatttc gacgactcaa gagaataaat gacgtttaag ccatgaaaca agctaaaatc
     8881 ctgcaaaaga cgagttttta cgggcgtatt taaagtgata atcataagat atctggtgcg
     8941 ggagacgctc aaaagccagc tggcgatact cttcatcttg cttttgatct tcttctgtca
     9001 aaagttagtg aggatcctcg gcgcagcggt tgacggcgat attccggcga atctggtgct
     9061 ctcccttctc gggttgggcg tgccggaaat ggcgcagctt atcctgccat taagcctgtt
     9121 cctcgggctg ctgatgacgc tgggcaaact gtataccgaa agtgaaatta cggtaatgca
     9181 tgcctgcggc ctgagcaaag cggttctggt gaaagcggca atgatccttg cggtattcac
     9241 ggcaatcgtc gcggcggtta acgtgatgtg ggcgggaccg tggtcatcgc gtcatcagga
     9301 tgaagtgtta gcagaagcga aagcgaaccc tggcatggcg gcattggcgc aagggcaatt
     9361 ccagcaagcg actaatggca gctcggtgct gttcatcgaa agcgttgacg gcagcgattt
     9421 caaagatgtg ttcctcgcgc aaattcgacc aaaaggtaat gcacgtcctt ctgtggtggt
     9481 ggccgattcc ggacatttaa cccagctgcg cgacggctcc caggtcgtca ctctcaacca
     9541 gggaacgcgc ttcgaaggca ctgcactgtt acgcgatttc cgcattacgg acttccagga
     9601 ttatcaggcg atcattggtc accaggcggt ggcgctcgac ccgaacgata ccgaccagat
     9661 ggacatgcgc acattgtgga acactgacac cgatcgtgcc cgcgcagaac tgaactggcg
     9721 tatcacgttg gtattcaccg tgtttatgat ggcacttatg gttgtaccgt tgagcgtggt
     9781 taacccgcgt cagggacgcg tactgtcgat gctgccagcc atgctgctgt atctactttt
     9841 cttcctgatc cagacctccc tgaaatcgaa cggcggtaaa ggtaagctgg acccgacgct
     9901 gtggatgtgg accgttaacc tgatttatct ggctttggcg attgttctca acctttggga
     9961 caccgtgccg gtccgccgcc tgcgcgccag tttttcgcgt aaaggagcgg tgtgatgcaa
    10021 ccttttggcg tacttgaccg ctatatcggt aaaactattt tcaccaccat catgatgacg
    10081 ctgttcatgc tggtgtcgct gtcgggcatt atcaagtttg tcgatcagct gaaaaaagcc
    10141 gggcagggga gttacgacgc gttaggcgca ggaatgtata ccttgctgag cgtgccgaaa
    10201 gatgtgcaaa tcttcttccc gatggcggct ctgcttgggg cgttgcttgg tcttgggatg
    10261 ctggcgcagc gcagcgaact ggtagtcatg caggcttctg gttttacccg tatgcaggtg
    10321 gcgctgtcgg tgatgaaaac cgccattccg ctggtcttgc tgacgatggc gattggtgaa
    10381 tgggtcgcgc cgcagggcga gcagatggcg cgtaactacc gtgcgcaggc gatgtacggc
    10441 ggctcgttgc tctccactca gcaaggctta tgggcgaaag atggcaacaa cttcgtctac
    10501 attgagcggg ttaaaggtga cgaagagtta ggtggcatca gtatttatgc ctttaacgag
    10561 aatcgtcgtc tgcaatccgt acgttatgcc gctaccgcga agtttgaccc ggaacataaa
    10621 gtctggcgtc tgtcgcaggt tgatgaatct gatctgacca atccgaaaca gatcaccggt
    10681 tcgcagacgg tgagcggcac ctggaaaacc aacctcacgc cagacaaact gggcgtggtg
    10741 gcgctggacc cggatgcact ctccatcagt ggtttgcaca actatgtgaa gtatctgaag
    10801 tcgagcggtc aggatgccgg acgttatcag ctcaacatgt ggagcaaaat cttccagccg
    10861 ctatccgtgg cggtgatgat gctgatggcg ctgtcgttta tctttggccc actgcgtagc
    10921 gtaccgatgg gcgtgcgtgt ggtcaccggt atcagcttcg gttttgtctt ctacgtactg
    10981 gaccagatct tcggcccgct gacattggtt tacggcatcc cgccgatcat tggcgcactg
    11041 ttgccaagcg ccagcttctt cttaatcagc ctgtggctgt taatgagaaa atcgtaatct
    11101 aacaagggag cgaaagctcc ctttattatt gttgacaaag tcaaatacga gttcgaacgg
    11161 tcccctcgcc cctttgggga gagggttagg gttagggtta gggtgagggg cacagatcac
    11221 gtctgactta cctttttctc ccccccagca aactccccaa catcccgcgt acaatctgat
    11281 tcgtcacctg gcgagcggcg cttttcgcca ttgtttgcac cacaccatct ttcttcccgc
    11341 cgcgtggtcc ggtagtgcca aacaaaatat ccttcaatcc accaagaata ccgtcatcca
    11401 ccgccacctc tttccctttc acggggggat tattttgctg ctcggtactg gcctgaaagc
    11461 ctttttgcaa catctcatag gcggattccc ggtccacctc atcctcatat ttgccataca
    11521 ccggagagtg attaatcaag ccattacgct catcttccgt caccggtccc atccgcgaac
    11581 aaggcgcaat caccatcgcc cgttccacca cagaagggct tcctttcgca tccagaaaag
    11641 agatcaacgc ctcgccggtg cccagttcct gaatcgcctt ttcggtatca aatgccggat
    11701 tgacccgcat ggtttgcgcc gcagctttta ccgctttctg atctttgggc gtaaaagtcc
    11761 gcaaagcgtg ttgaacgcga ttaccgagct ggccgagcac gttatccgga atatcggacg
    11821 gattttgcga aacgaaccag acgcccacgc cttttgagcg aataagcctt atcacctgct
    11881 caatcttatc cagcagtacc tgcggtgcat cgttaaacag cagatgtgcc tcgtcgaaga
    11941 aaaacaccag ttttggtttc tccagatcgc ccgcttccgg caattgttca tacaactccg
    12001 agagcatcca caacaggctg gcggcataca gtttcggcat ctgataaagc ttctcggcgc
    12061 tgaggatatt gataacgcct ttaccgttgg catcggtgcg catccagtct ttgatatcca
    12121 gcatcggctc accaaagaag tgtgctgcgc cttgctgttc cagcgacaac agcccgcgct
    12181 ggatggcacc aaccgatgcg ctactgatat ttccgtactg attctggaaa gatttggcgt
    12241 tatcgccgat gtactgggta atcgcccgca aatctttaaa gtcgagcagt aacagcccct
    12301 gatcgtcagc aatgcggaag atgatattca gcacgccaga ttgcacatcg ttgagattca
    12361 acagccgcgc cagcaacagc ggccccagat ccgaaaccgt cgcccgcacc ggatggcctt
    12421 tctcgccaaa gatatcccac accaccaccg gattggcatg cggttgccag tcattgacgc
    12481 cgatattttt aagccttgtg agcagttttt ccgacgccgt tcctgcctgc gcgataccgg
    12541 tcagatcgcc tttcacatca gccataaaca ccggcacgcc gatttccgac aatgactctg
    12601 ccagtttttg cagcgtaacg gttttacccg tccccgttgc gccagtaatc agcccgtgac
    12661 ggttagccat tcccggcagt aaaaacagtt ctgtgtccgg cgtgcgggca attaacaggg
    12721 gttcgctcat gagatttcct ccagtttcct gcctggagta taggcaacgg ctgtgcactc
    12781 ttcaccgcta aattcagcag attgcgagac gccttccaaa gagatttaag tgccgggcga
    12841 cagcacccgg caaagggatc agaagtcggc tttcaggact actcggtagc gagctttacc
    12901 atcgcgtaca tgctgaatgg cgtcgttaat tttcgacatc gggaacagtt cggttgtcgg
    12961 cgcaaccttg ctgcgggcgg caaagcgcat cagctttcgc agctcataag gcgtgccggt
    13021 agcagaacca gagatgctgc ggtcgcccgc aattaacgta aaggccggaa cagacagcgg
    13081 cgtgagaacc gcaccgaccg tatggaaatt accgccatag gtcagcgcct caaaataagg
    13141 ctgccagtcg aggctgacgt tcacagtatt gataatgaga tcaaactgcc ccgacagggc
    13201 tttcagtgcc tgcggatcgc ggctattcac cactttatcg gcacccatcg ccagtacttc
    13261 ctgctctttc gccggattag aactaaaggc cgtcacctcg catcccattg cgtgcagaag
    13321 ttttatagcg atatgcccca gcccgccaat accaattacc ccaacgcggc tggtagcagt
    13381 gatatggtgc atcaacagtg gtttaaagac cgtgataccg ccgcacaaca gcggcccggc
    13441 ggactcgata tcaatatttt ctggcagtgg aatcacccat tgccagtccg cacgcaactt
    13501 ctcggcaaag ccaccgcgat tcataatcgt cggcaccgca ccttgctcgc agttgatctg
    13561 attaccgcta atacaggcgt cgcagtgacc acagctacgc gccgtccagc caatcccgac
    13621 acgctgaccg acctgcaaac ctttatcctg cgcggcgctc ccgagtgcca ccacgcgccc
    13681 aatcacctca tgcccggcaa ccagcggata ttgtgaaaat ccccattcgt tatcgatcat
    13741 cgacagatcg gaatgacaaa tcccgcagta atccacctgc acttcaacat cttgtggctt
    13801 cagctcaccg ggatcgtact cataaacttc cagttcgccg cccgcttctt ttgcggcata
    13861 gctttttatc atcgacattt ttgggtcctt ctctggtgtt gtttgggaag tgtagagcat
    13921 ggcagggcgg ggtgcctgga gtgtgacaaa ggttacatat cgctgagtgt aatgctgaaa
    13981 atttcagcac ttagcgaggt gggtgcaagc tggcgcttgc atggtggcgt gcgacaggta
    14041 taatccacaa cgttttccgc atacctcttc agtgccgaag tggcgaaatc ggtagacgca
    14101 gttgattcaa aatcaaccgt agaaatacgt gccggttcga gtccggcctt cggcaccatc
    14161 ggaacatcaa tagacgtcaa cggacgtctt tttttgtgcc taaaatccag tatccgcaag
    14221 gctttcctcg ctttttcact caacctaagt caacctgatt caatctacat caacttactg
    14281 atgcgggtac aactgcgggt atttcccggt tcgataatgt ttgtacccac acagaaccct
    14341 tgaaaggata ctcatcatgg ctctgagtga tgtgaaggtt cgttcggcta agcctgaagc
    14401 aaaagcctat aagctgaccg acggtgaagg catggttttg ctggttcacc ctaacggctc
    14461 caaatactgg cggctacgtt atcgctttgg cggtaaagag aagatgctgg cattggggaa
    14521 ataccccgaa gtgtcgttgg cggatgccag ggcacgccgg gatgaggctc gtaagctttt
    14581 agctaatggt gtcgatccca gtgaaaacaa gaaagctgtt aaggtagaac aggaacaaga
    14641 ggcgataacg tttgaagtag tcgccagaga ctggcacgcc agcaatcaga agtggtcggc
    14701 atcgcatagc gctcgtgttt tgaaaagtct ggaggataat ctctttactg ctatcggtaa
    14761 gcggaacatt gcggaactga agacgcggga tcttcttgta cccattaaag cagtcgagtc
    14821 atccgggcgg ctcgaagttg ccgcccgttt acagcagcgt actaccgcga ttatgcgctt
    14881 tgccgttcag agcggcttaa tcgactacaa ccccgcgcaa gagattgcag gtgcggttgc
    14941 tacggcgaaa agacagcacc gtgcagctct tgaactgaac cgtatacctg aattacttca
    15001 tcgcatagat cactattctg gcagaccgtt aactcgactg gcggttgaac tcactttatt
    15061 ggttttcatc cgttcgagcg agctgcgttt tgcccgctgg tcagaagtag attttgaaac
    15121 ggctatgtgg acaatcccag gtgagcgtga gcaactggaa ggagtcaagc attctcagcg
    15181 tggctcgaag atgcggacac cacaccttgt acctttgtcg cggcaggccc taagtatttt
    15241 gggaaagatc aaaagcatga gcggaaatcg agagctgatt tttgtgggcg atcacgatcc
    15301 acgtaagccg atgagtgaga atacggtgaa caaggctttg cgcgtaatgg ggtatgacac
    15361 gaaggtcgag gtttgtgggc atggcttcag gacgatggct tgtagttcgt tgattgagtc
    15421 gggattatgg tcgagggatg cggtagagcg gcagatgagt caccaggagc gtagctctgt
    15481 gcgagcagct tacatccata aggcggaaca tcttggggag cgtaggttga tgtttgaagt
    15541 ggcacactga atttggccac ctgaacagag gtgatatgct cacctcagaa caacacaggt
    15601 gccataatga aaaaagaaat ttcagcgcag agtttaaacg cgaatccgct caactggttg
    15661 ttgaccagaa atacacggtg gcagatgccg ccaaagctat ggatgttggc ctttccacaa
    15721 tgacgcgatg ggtgaaacaa ttacgtgatg agcggcaggg aaaaacacca aaagcctccc
    15781 ccattacccc ggaacaaatt gaaatccgtg agctcaggaa aaagctacaa cgtattgaaa
    15841 tggaaaatga aatattaaaa aggctaccgc gctcttgatg tcagactccc tgaacagttt
    15901 tcgataatcg ggaaactcag ggcgcgttat cctgtggcca ctctctgcca tgtgttcggg
    15961 gtccatcgca gcagctacaa atactggaaa aaccgtcctg aaaaaccaga cggcagacgg
    16021 gctgtattac gcagccaggt acttgaactg catggcatca gccacggctc tgccggagca
    16081 agaagcatcg ccacaatggc aacccagaga ggctaccaga tggggcgctg gcttgctggc
    16141 agactcatga aagagctggg gctggtcagt tgccagcagc cgactcaccg gtataagcgt
    16201 ggcggtcatg agcacgttgc tatcccgaat catcttgagc gacagttcgc cgtaacggaa
    16261 ccaaatcagg tgtggtgcgg tgatgtgacc tatatctgga cgggtaagcg ctgggcgtac
    16321 ctcgccgttg ttctcgacct gttcgcaaga aaaccagtgg gctgggccat gtcgttctcg
    16381 ccggacagca ggcttaccat gaaagcactg gaaatggcat gggaaacccg tggtaagccc
    16441 gtcggggtga tgttccaagc gatcaaggca gtcattatac gagcaggcag ttccggcagt
    16501 tactgtggcg ataccggatc aggcagagta tgagtcggcg tggaaactgc tgggataaca
    16561 gcccaatgga gcgcttcttc aggagtctga agaacgaatg ggtgccagcg acgggctatg
    16621 taagcttcag cgatgcagct cacgcaataa cggactatat cgttggatat tacagcgcac
    16681 taagaccgca cgaatataat ggtgggttac caccaaacga atcagaaaac cgatactgga
    16741 aaaactctaa cgcggaggcc agttttagtt gaccacaaca gactacctga agggagccgc
    16801 ggtcgcctag cagttgcagt agcaggagat catccagccg cagtacaggt cacgatgact
    16861 ctggttaatg ataccggctt tgaccccgta ttttccggct ctatcgctga atcatggcgt
    16921 cagcagccgt gcacaccatc ctattgttgt gactgggagg ctgccaccat gcttcgcgct
    16981 ttccctctgg cgaaaaaggg agaaggacgg gcccgtctgc cttcacttta tgccagcttc
    17041 ggtaagctgg gtgagacacc gactcatgaa gatatcattg ataacaatcg atccatcaac
    17101 tggcctgtat aacgtggctg ccggtgatta agaaagctgc acctacctaa gtagtagcaa
    17161 acgcacactt tttagaaaaa tcgatggtca gaaactggat tagcaattcc gttccagggt
    17221 tgcttttgat ttacgttggc gtctgatcat tgatttatcc tcaaaagccc aacctcattg
    17281 gtaatgaacc agctccgtga atgtccgctc tggcacagag cgaaattttt tgatctcccc
    17341 ccctgaaatc taaacttagt catgtcacgt ttttgggttt ctaaaatttt aacttcgcgt
    17401 ttttcgttgc cgtaagggtt atacagaaat gtccgttaag cagagttcaa aattgattgc
    17461 cgtgatcacg actggtttga aagccgcgcc caagcctgta cagctctggt ttgcgttgat
    17521 tatgaacctg tcagcctaaa gcaagcggat ggacgatgag tattggtaat ctttcagagt
    17581 ccggaaaagt tcagccccag tctgaacagg cttgctggcg ccagtccagt ttcattcagt
    17641 cgtggtttgg ttcttacggc ctgtgcaatc tacctcatta ggcacatcgg cctgccagat
    17701 accggctcgg ggtgtatttc cgcttccacg ctgaatactg ttctcagcaa tcctggggtc
    17761 atcacctctt ctggtgtgcc ttgcgccata acatgtccgt ttgccattac caccagttga
    17821 tcgcagtacc ggctagcctg attaaggtcg tgcagcacag cgaccaccgt tttcccctga
    17881 gtccggagtt cgcccatcaa ccgcatcagg tccacctggt gattgatatc aagataggtg
    17941 gttggctcat caagtaatac aacgggcgta ttctgggcca ggaccatcgc cagaaatgcg
    18001 cgctggcgct gaccgccgga aagctcggtt aaccgacgaa cggcaagatg attgatccgg
    18061 gtctggttca tggcgacatt aactcgtgca ttgtcttcag cggagagacg cccccagagt
    18121 gactgccagg gattacgacc atacgaaacc agctcctgga ctgtgatccc ctctggcgtt
    18181 aaatggtgct gaggcagcag cgaaagcctg cgggccaact ggcgtgatga gagcatattt
    18241 atgggattat cgccgagaaa tacggtgcca gactgcggca ttaaaagccg cgaaaaacag
    18301 tttaacagcg tcgatttccc gcaaccgtta ggaccgatca gggcggtgat cttccccgtt
    18361 ggcagtgaga gtgaaacgtc gttaagtacc ttgtctgtcc cgtaactgac cgtcagattt
    18421 tcagttcgta aagtcattta tcgcattctc acaagcaacc agacaaacca cggcgcaccg
    18481 ataatggcgg tcagcacgcc aaccgggagc tccagtgggg gatgaataat tctcgccagc
    18541 agatcggcaa ccaccaacag caacgcacct gtcagggccg aaacaggcag cagtctgcgg
    18601 tgacgtccac cggtgatgct acgcatcata tgcggcacca cgagaccaat aaagctaatc
    18661 gggccgcagg cggccacgcc ggtagatgtc atggcgacag ctagtaacaa agcccagaat
    18721 cgggtatggg gcaccgacac accgagcgtg gtggcgcgcg catcgccgag tgcaaggagg
    18781 tcgagatcgc ggcaaaaact caggctcagc ggcagaaata aaatcatcag cgggatggca
    18841 atcttcacaa agctccagtc acggccccat aagctgccgg tcagccacag cagggcgttg
    18901 ttcacatcct gcgggcgcga gagcatcaga taatccgtca ggctggccca gcatgcagaa
    18961 agcgccacgc cggtgagcgc cagcttcatc ggctggtggg tctttgccag catcttcagc
    19021 aatatcaacc ccgccatgcc gcccgcaaag gccagcagcg gcagcaccat cacgggcagt
    19081 gacggcataa gaagtagagc ccccacagag gccaggctgg cggcatggtt aacgccgaga
    19141 atatccggtg atgccagagg gttgcgcaca atcccctgta tcagcacgcc cgccacggcg
    19201 agggctgcac cgacaaacag tgccagcagc aagcgcggca gtcggtactc catcaataca
    19261 taataatgct cgcgtccggc ctgccagtcg gtcagcagcg cgcgccacgg cacggggatc
    19321 actcccatat ggagtgataa cagcgcacag cccgccaggg caagggtgat gaaaataacc
    19381 agcgcaattt tcatcctcgc ctcctcacaa gccagacaaa gcaagggctg ccaatcagcg
    19441 ccagcactgc gcctgcgggc agatctccgg ggaaggccag cgcgcgtgcg agtacatctg
    19501 ccagcagcat cagcgtggcc cccagcagca tgctcactgg cagtacgttg cgctgatcga
    19561 agcctgccca gaagcgcgcc agatgtggca ccagcagacc gataaacgcc accggacctg
    19621 ccacgctgac gcacgcacca accagaagca gcactaacat attgatgacc aaacgtagcc
    19681 tcgtcaggtt cactcccagc gtatgggcgg tgctgtcgct gaggttgagc aggttcagtt
    19741 gattcgccag cagcaacacg acagggactg cagtgaccac caccggcaag agctgccaga
    19801 catcctgcca gcgggcgtgg gacactccgc ctgccagcca gtaaaagatg ccgtaagcat
    19861 gatcttcggc cagcagcagg gtgatgcggg taaggcccat acaaaaggcc gacagcgcga
    19921 tacccgcgag gatcagtttg tttctgtcat gggtatgacg aaatccgcct cctgcggtca
    19981 tgaccagcag ccagctcacg ccgcccccgc atgccgcgat gaacgacaga gaatagcctg
    20041 caatcgacgt cggactcagc gcgctggtaa gcgccatagc cagcgccgcg ccgctgttaa
    20101 tgccgagcag tgaaggagag gccattgggt tgtgggtcag ggtttgcagc agcgtgcccg
    20161 cgagcgccag gcttgcgccg atcagaacgg cgaccaggct tcgtggcaaa cgaaggtttt
    20221 gcaccagcgc ttctggtagc gttggcgtgt gtccaggcag cagggcgcgg gttgcatctg
    20281 ctccggaaac aggaatggcc gagtagcaaa acagactcag ccagaaaata ataataagtg
    20341 ctgcaacggg aagcccccac agcagcaccg ggtgttttat cgcggtcatt tcacaacggt
    20401 aagcggctga tggtggaaga tttttaccgt gtcagcggca atacgctctg cagcaaaaat
    20461 accgcgcatc cgcgcccagg tgttactgtc gatcgaagca acctgctgct tctgcgcggc
    20521 ggttaacatc tgccagagcg gatcttgttg ccagcgttta acaatgctct cttcgcgata
    20581 gtgggcaacc agcagccagg caggattgac cgccagcagt tgctccaggc cgatggacgg
    20641 catggacgca cccgccatcg cagcgggaac gttcagcccc agagaggcca gcacgctgcc
    20701 ggtccaggtc tcctgagtat gcaggttgaa ttgctgttcg cgtgatgtgc caaaggccac
    20761 gcgtgtccct ttgggaagct ggctggccca ctgcgccatc ctctctttat gttgttccag
    20821 acgtgcctgc atctctcgct ttttacccac catttcgccg atgatagccg cagattgcaa
    20881 attttcagcg taggtttcgt tgcgggactt aagcagcagt accggcgcga tttgctgcaa
    20941 ggcgatgtaa acccccgcat ggcgactgct gtcggcaatg atcaggtctg gtttcagagc
    21001 ggcaatggct tccaggctcg gctgcgcgcg cgttccgacg gactgccacg gtttcaggtg
    21061 cgcacgcact tcgggcagga tgcgttttgc atcgttatcg tcggcaatac cgatcgggct
    21121 gacgtccacg gcggccagcg catcggcgaa cgagagttcc agcaccacaa tccgttgtgg
    21181 cgttttttcg agtgtaaacg tgccgtgttc gtcctgaacc atggcggcaa aggcgtggct
    21241 gatcaccagc agcaggcctg caaaaagaaa acggataaat gccaacataa tcacattcca
    21301 gctaaaagcc cggcaagtcg ggcgttaaca catcagaact tcaacgaccc ctgcatatac
    21361 agcgtgcgcg gctggcctgc atagatgcct ttgttgttgt cgtcataaga gcggatgaag
    21421 tagtcctggt cgaagatgtt tttcacaccg aatgccaggt tcagatctgc catctgcggg
    21481 ccaaagtcat acgccacgcg tgcgccccag agcatgaagc cgggaatgcg gccggtactg
    21541 ccgtcggcgc tctctttcac cgtattggcg ttatccgcaa actggctgga ctggaaatcg
    21601 ctgttcagat tgaacgtcca gtttcctggc ttgtagtcca cgcccagcgt gcctttatgt
    21661 ttcggggaga atggtaccag attgccgtag gtgtcgcctt tctcgcggat ttccgcgttc
    21721 acatacgcat agctggcgta gatggaaacg ttatcaagcg ttggcgttag cgtacccaga
    21781 tcgtaacgtg cctgcgtttc cagcccggta tggcgcgttt tgccacgtgc agtgacggtg
    21841 tcgttggtct ggttggagtc gtactgattg ttaaagttaa tcaggaacag ccccatttcc
    21901 gccgtcagcg cgccgtcgtc gtagcgggta ccgagttccc aggttcgcgc tttttccggt
    21961 tccacattgc cgctttgcac agccttgcca atctggctgt actgtacggt gccgaacgag
    22021 ccttcagtgt ttgcataaag attccagctg tcagtcaggt gatagagcac gttcaacgcc
    22081 ggaagcggtg cgttatagct cacttcttcg tgcgtgcctg tgatggcgtt gttctggtat
    22141 gactcgatat gttcgaaacg catacccggc gtgatggtcc agttgccgat gtcgattttg
    22201 tcatccagat accaggcgtg cgcctcggtg ccggaacgcg tatcgcggtc gtaagggctt
    22261 gagccggacg gcaactgccc gctgctggtg gcggtgtagt aacgcatttc atgcgttgat
    22321 tcattcaaat agcgatagcc cacgcccact tcgtgcgcgg aagggccgat cataaagatc
    22381 tggctgtagc gtggctcaat accgcgcacc cagtagttac gcggcgagag ggtgatgcgt
    22441 ttgccttgct ccaggtagcc gctgcgcagg gtttgggtgt agaacccctg aatgttgaat
    22501 ttatgctggc tgtctggctg gaactggtag cccaggctcg ccagcttgcg acgaccccag
    22561 aagcggtcat acgggcgggt ggattgccag cgatcggcgt cgtaatccgc gcgagacagg
    22621 ccaccgggca tgtcggcttc accgtcgtaa tattgcagca ggctgttgaa ggtgtgcacc
    22681 tcatccggcg catatttgct tttcagcatc aggtcgtcga tgcgggtggc gctgtgctcg
    22741 cgccagtcac tgccgcgcgt gccggagtag agcagcgcgg tgccaaaacc gttgtccgct
    22801 gtgccgccca ccatcaggtt gtgcgtctct ttcgggttgt tttgtgaaga ggttgggctg
    22861 agcagacctt ccacgcccgc ctcgatacca aagtcctgcg gaatggcacg ggtaacaaag
    22921 ttcaccacgc cgcccacgct ctgcggtccg taacgcaccg caccaccacc gcgtaccacg
    22981 tcaatggcat ccatgttgcc gagcgaaacg ggagccagtg aaagctgcgg ctgaccgtaa
    23041 ggggcgaagg ggacggggat gccgtccatc aggacggtcg agcggctggc gaggcgcggg
    23101 ttcaggcccc ggatgccaaa gttcatcgcc aggtcgtggc tgccggtgcc gttgttttcc
    23161 ggcgcgctga cgccagggat gcggttaagt acctcacgca tggtggttgc gccggttttg
    23221 gcgaaatcct cacggcggat cacgtcacgc gcgccagcat gttcaaatac gtcgttttca
    23281 cgcgcatcac ccagccagtc gccgaccacg gtcagggcat cttcttttgg tgcgggcgcg
    23341 ggctccagcg tccagctgtt atttcccagc ggttttacct gcagtccgct gccgtccagc
    23401 agttgttgca ggccgctctc gacgtcgtaa tcgccgtgca ggccgttgct ctgcttgccg
    23461 cgcgtcaggc tggcgtcaac cgagagggta aatccgctgt gtgcggcata ctgattgagc
    23521 gctttatcga gcgatcccgg tgcgatatta acctgtgcag caaaagcgga aaacgagaga
    23581 ccggccagcg gcagcaggct caggcgaatg gtgttaacca aaggtgttgt tttacgaaaa
    23641 acgcgtaacg gcgtcatacc ttccccatca tcatttttgt tgtgttcagc tatgagtcga
    23701 acgagaaggg taaaaaggac aatcgaaata agaattattt tccttacagt ggtgaaatgt
    23761 ttatccagta ccgcgtaata gactgaattt taacgggaag cgtttgcgcg ataacgttca
    23821 ggatcgcatc ggtatttttc agcgggaacg tcccgctcag gcgcagcccg gcaacggcgg
    23881 gatcgcagcg cagcacgccg ttgcggtaac gggttagcgt ggctatcacc tcacccagcg
    23941 gtttatcgct gaagctcagg atatccttcg tccagcttgt actctcgtca tccagcggtt
    24001 tcactgcgcc aaactcagag gcgctgaact gcaggctttc accagcgttc acgatgcgtt
    24061 tttgcgcggg ggcactggcg agaagcactt ccacggcgtg ctgctgcacg tcaagctgcg
    24121 tgaaattatc ctgctggcgg acggtaaatt ctgtccctaa agcagtgagc tggccctgac
    24181 gggtcaggac acggaagggg cgttgcaggg catctttcgc ggtagtaatg gcgatttcac
    24241 cgtaccagag ccggacggtg cgctgatgcg catcaaaacg cacatccgcc gcgctttggg
    24301 tattcagcgt gagcagggag ccatcttcca gttgctgacg gctgactgtg cctttggcgg
    24361 tgcggtaatc tgcccgcaga ccttcgccgg tttccgactg ccagagctgc cagcctccgc
    24421 cagcgccgag caacagcagt aatcctttca tcacgtgacg gcgggtgagg cgggtatcgt
    24481 gcaacgcccg gctggcaacg tcgccaggca caccgccaag ctggttgcgc aggttttcaa
    24541 cctgctgcca ggcccactgg ttatcctgat cctgttcata ccactgttgc cagcgcgctt
    24601 cctgttgtgg gctgacgcgc tcgccgctta gcacggcata ccagtgggaa gctgaacgca
    24661 gcgcctgacg gcgggaatcg gttaacaaag gattcataac ccatactcca gacggaacag
    24721 caggcagtgc tcgacggctt tcgccacgta ttttttcacg gagctgatgg aaacaccgag
    24781 tttgtgcgca atctcgctgt atgtcagacc atccagttgc gaaagcagaa acgcttcacg
    24841 tgttttgccg tttagcccgt ccagcatgct gtcgaggagt tgtagggtct cgagttggct
    24901 ttcgcgttcc tcaggtgaag gcgctccccc ctccggcata agcgccagca tctccagata
    24961 cgctttttcc agcgcgtttc ggcgaaacag gtccaccatc acgcgtttgg cgatagtgca
    25021 caggaaggag cgaggatcgc ggatcgtcga gagcgtttcg ctgaccatta cccgcaaaaa
    25081 agtgtcctgg gcaatgtcat ctgcatcaaa agcagactgg agtttgcgcg tcagccagct
    25141 tttcaaccag ccgtgatgtg tgccataaag cgactcgaac gttaaggaag ctgtggtagt
    25201 ggcgcggtca gacatgcgga gtgcatcaaa agttaattat cacgtagtca tattaatatg
    25261 agaatggtta tcattacaat tggaaataaa attgtttcca atagacattt ttaacatgtt
    25321 gtttttctaa gtgttataag gtaggtataa aatgggatgg agcctctgct tctggcatgt
    25381 gtcggtcaga atgactcatg atgtggtctg ctattattga catcctcact gccctaaagg
    25441 atggggattt cggtaatgct gccaacttac tgatttagtg tatgatggtg attttaaggt
    25501 gcttgcgtgg cttccatttc catcagatgt ccttcctgct ccgctactga aggcgtggtg
    25561 cgtaacggca aaagcactgc cggacatcag cgctatctct gctctccttg ccgtaaaaca
    25621 tggcaactac agttcactta caccgcctct cagcccggta agcaccagaa aatcattgat
    25681 atggccatga atggcgtcgg atgtcgcgcc agtgcacgca ttatgggcgt tggcctcaac
    25741 acggttttac gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc atacaatcgg
    25801 gcagtgatgt gattgtctgc gctgaaatgg acgaacagtg gggctacgtc ggtgctaaat
    25861 cacgtcagcg ctggctgttt tacgcgtatg acaggatacg gaggacggtt gtggcgcacg
    25921 tcttcggtga acgcactctg gccacactgg agcgtcttct gagcctgctg tcggcctttg
    25981 aggtcgtggt atagatgacg gatggctggc cgctgtatga atcacgcctg aagggaaagc
    26041 tgcacgttat cagcaagcgt tacactcagc gcattgagcg acataatctg aatctgagac
    26101 aacatctggc aaggctggga cggaagtcac tgtcgttctc aaaatcggtg gagctgcatg
    26161 acaaggtcat cgggcattat ctgaacataa aacactatca gtaagttgga gtcattacct
    26221 cctcccgaac tcaaagctat catacagata attactggtt aaccctatat agggcatttt
    26281 aaagtgttgt gatcttaccc agcaatagtg gacacgcggc taagtgagta aactctcagt
    26341 cagaggtgac tcacaagaca aaacagtttc aaccagtaaa aaaacgtaaa caccattcgc
    26401 atgaatttcg tagttaagat ctgaagcttg cagaatgcat cggtgttact gccggagtcc
    26461 gtgaacttag tctgcatgaa tcaccacagc tttacatctg tcgcagtaaa cagcaaaatc
    26521 agcaaactta cttaattata tcgagtgcga ttacaatcgc tggcgatggt acagttggtg
    26581 tggcggtctc agtccggaac aatttgaaaa tcagaatctc acttaaaacg gcatccattt
    26641 tacaaaagat ggccgtctgt gtatttgtaa tgaatacctt ctacatgttt agagtgcata
    26701 atccacgaca aggcgatatc ctaaaaatta gctagcaggc aactttgatg gcatagataa
    26761 tgtttttagt aaaaaagagt tgactgaata taatacagcc tcttcctaca taagaaggaa
    26821 tgtgctcaca aaaaaaacca ttagtctgct tcctgttctt acaataatca gtattatgta
    26881 ttttatttat atagcaaaat atgcttgtta cacctgtcat aattaggcat ggtaaaatta
    26941 ttgactgaac cctgggggag aaagatgcac agtgattatt ataatatggt ttttggtgaa
    27001 aaattggcca atattttata tgaagctaac tctcaatttt tttacgagag aaatgtcata
    27061 gaagaggcag tcaacgcttt attctgtgag cgtgaaataa taaacaacaa gaacataata
    27121 aagaagctga tgttcttttt gtcagatgtt aatcacacca agaaagatgt tgttcagagc
    27181 gcacttaata ttatcattga cattacatca ggtgatattt gagattttac ataaaagata
    27241 atctctcaag gagtcttatc tgaaaacgaa atatatagtc cctctcgttt tgtaatatcg
    27301 attggctttg ttgaatcggt tacgtacccc ggagccggtc atattttcag gtaatacaca
    27361 cgctttcaga catttcggct ttcgtcattt tggtatcatg ggcatagctt ctgctacctg
    27421 agcatcataa tcacgcagct tcagtgaagc agtgaccacc tgtttaaccc ggtgtatcgt
    27481 tatgatctta tccagcaata atggacacgc ggctaagtga accgccccgg gtttcctgga
    27541 gagtgtttta tctgtgaact caggctgcca gatcatcgtt tccgatggaa gcataataag
    27601 ctttttctgc ttctgccggt gggatatggc cctgcctttc cagcaatcgt cgattgttat
    27661 accagtccac ccacgtgagt gtggtcagct catcatactg acacgtgcat tacccctacc
    27721 gcgataatgc aaccgtacaa tatagggcgt ttcctaaacg cagccctcat cactccaatc
    27781 caacaagaca cagcattttt gatcacgaag aaagacaaat aatttccgta aatgtttccg
    27841 ttgttacaaa taacaacagt gttttacatt tctttggcct caggcccgcg catgttgcca
    27901 ttcgatgtta ttttcctgac tcgtttcttc tttatggtgt agtgtgatct tcactttatg
    27961 gatgctaaca atatgaaaag aaaaattatt cccgtgctaa tcggttgtac gctctctttc
    28021 tctgccctgg cggcacaacc tacggctgag cgctatgtcg tcagttttcc agaaggcacg
    28081 cacgtgaact acgccggcgc atttgccagt gcattcccga acggccttcc ggttgggata
    28141 ggttctggcc tgctgttcac cggtaaacag ggcgatgccc tgacattcgc aaccattacc
    28201 gatcgcggtc cgaacgcgga ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt
    28261 acgccagatt tcgcgccgct gctgatgacg attcgtgtac aaaacggcaa agcggaggcc
    28321 atcgatcccc gtcctctgca tgacgataaa ggcgcgatca acggtctgcc gctggcaagc
    28381 gacgtgattg gctccaccaa tgaagtggcc ttcagcgaca cgttacatag actgaagggg
    28441 gataatcgcg ggctggatac ggaagggatc actccggatg gtaaaggagg ttactggctg
    28501 tgcgatgagt atggcccgtt cctgattaac atcgacagta aagggaaaat cctggcaatc
    28561 cacggtccgc aggcggcgga aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc
    28621 aaatggcgtc aggcgaaccg cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt
    28681 attgtcgccg tgcaaagtac gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt
    28741 acgcgtctgg tgagcttcga tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc
    28801 gacagcgcgg cctacagcaa aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat
    28861 aaccagcaca tcctgctgat cgaacaggga agagataaaa acaacagaat gcgtaacctg
    28921 atttacgagg tcgatctcaa taaggcgagc gacctgtcag gcttcgacaa gccgggtgag
    28981 tatccggagt ttgatgatga gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa
    29041 acgcaggttg ttgatctgcg ctccttgggc tggcagcagg agaaggctga agggttggcg
    29101 ctgatcgaca gcaaaacgct ggcggtggcg aacgataacg actttggcgt gaaggtggcg
    29161 atgcaacatc cggtcgaggg caagacattc aaggattatc gggtgaacgc ggaaggaaag
    29221 ctgacgcttg acgacaagca ggttgaaacg accctcagag tgaagccact ggagaaaccg
    29281 gagtctgaca gtgaactgtg gattgtgacg ctgccggaag cgttgaaata agtttataag
    29341 gtacggacag cagtatcgct tagtttttca aattcatgta tgtgaacaat attggggttc
    29401 cggcgtagac ccccaatgaa agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt
    29461 catcccatgg tggcagccgg ttaactttcc tgctacacca gtggaaaaag gccataagca
    29521 gtccgaacaa aagtaagcgt acagcgaggg ccgt
//
LOCUS       DAZKHC020000054        29433 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZKHC020000054 DAZKHC020000000
VERSION     DAZKHC020000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.054"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..623
                     /locus_tag="ACHOSM_RS13760"
                     /old_locus_tag="pgaptmp_002752"
     CDS             <1..623
                     /locus_tag="ACHOSM_RS13760"
                     /old_locus_tag="pgaptmp_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4559237.1"
                     /translation="QRLGGQGASGNSGSFSLKILQPFVGSVVIPPMALARLFECYNIP
                     AGDSCTTTGTPVLVYYLSGTINSLGSCSVNAGETIEVDLGDVFAANFRVVGHKPLGAR
                     TAELAIPVRCNTGNAGLVNVNLSLTATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPA
                     GGTLPLSIPDDADSIARMNVYPVSTTGVPPETGRFEATATVRINFD"
     gene            635..1177
                     /gene="ycbU"
                     /locus_tag="ACHOSM_RS13765"
                     /old_locus_tag="pgaptmp_002753"
     CDS             635..1177
                     /gene="ycbU"
                     /locus_tag="ACHOSM_RS13765"
                     /old_locus_tag="pgaptmp_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4559238.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            1185..1700
                     /gene="ycbV"
                     /locus_tag="ACHOSM_RS13770"
                     /old_locus_tag="pgaptmp_002754"
     CDS             1185..1700
                     /gene="ycbV"
                     /locus_tag="ACHOSM_RS13770"
                     /old_locus_tag="pgaptmp_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4559239.1"
                     /translation="MLKRIIWILFLLGLTWGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            1666..2403
                     /gene="ycbF"
                     /locus_tag="ACHOSM_RS13775"
                     /old_locus_tag="pgaptmp_002755"
     CDS             1666..2403
                     /gene="ycbF"
                     /locus_tag="ACHOSM_RS13775"
                     /old_locus_tag="pgaptmp_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGU4559240.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGNVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVLPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            2514..3524
                     /gene="pyrD"
                     /locus_tag="ACHOSM_RS13780"
                     /old_locus_tag="pgaptmp_002756"
     CDS             2514..3524
                     /gene="pyrD"
                     /locus_tag="ACHOSM_RS13780"
                     /old_locus_tag="pgaptmp_002756"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HGU4559241.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            3698..4240
                     /gene="zapC"
                     /locus_tag="ACHOSM_RS13785"
                     /old_locus_tag="pgaptmp_002757"
     CDS             3698..4240
                     /gene="zapC"
                     /locus_tag="ACHOSM_RS13785"
                     /old_locus_tag="pgaptmp_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HGU4559242.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(4237..5346)
                     /gene="ycbX"
                     /locus_tag="ACHOSM_RS13790"
                     /old_locus_tag="pgaptmp_002758"
     CDS             complement(4237..5346)
                     /gene="ycbX"
                     /locus_tag="ACHOSM_RS13790"
                     /old_locus_tag="pgaptmp_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HGU4559243.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSTYVRFAAFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            5590..7698
                     /gene="rlmKL"
                     /locus_tag="ACHOSM_RS13795"
                     /old_locus_tag="pgaptmp_002759"
     CDS             5590..7698
                     /gene="rlmKL"
                     /locus_tag="ACHOSM_RS13795"
                     /old_locus_tag="pgaptmp_002759"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HGU4559244.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            7710..9617
                     /gene="uup"
                     /locus_tag="ACHOSM_RS13800"
                     /old_locus_tag="pgaptmp_002760"
     CDS             7710..9617
                     /gene="uup"
                     /locus_tag="ACHOSM_RS13800"
                     /old_locus_tag="pgaptmp_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4559245.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRMLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            9747..11000
                     /gene="pqiA"
                     /locus_tag="ACHOSM_RS13805"
                     /old_locus_tag="pgaptmp_002761"
     CDS             9747..11000
                     /gene="pqiA"
                     /locus_tag="ACHOSM_RS13805"
                     /old_locus_tag="pgaptmp_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HGU4559246.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            11005..12645
                     /gene="pqiB"
                     /locus_tag="ACHOSM_RS13810"
                     /old_locus_tag="pgaptmp_002762"
     CDS             11005..12645
                     /gene="pqiB"
                     /locus_tag="ACHOSM_RS13810"
                     /old_locus_tag="pgaptmp_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HGU4559247.1"
                     /translation="MESNNREAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            12642..13205
                     /gene="pqiC"
                     /locus_tag="ACHOSM_RS13815"
                     /old_locus_tag="pgaptmp_002763"
     CDS             12642..13205
                     /gene="pqiC"
                     /locus_tag="ACHOSM_RS13815"
                     /old_locus_tag="pgaptmp_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HGU4559248.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            13461..13628
                     /gene="rmf"
                     /locus_tag="ACHOSM_RS13820"
                     /old_locus_tag="pgaptmp_002764"
     CDS             13461..13628
                     /gene="rmf"
                     /locus_tag="ACHOSM_RS13820"
                     /old_locus_tag="pgaptmp_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HGU4559249.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(13698..14216)
                     /gene="fabA"
                     /locus_tag="ACHOSM_RS13825"
                     /old_locus_tag="pgaptmp_002765"
     CDS             complement(13698..14216)
                     /gene="fabA"
                     /locus_tag="ACHOSM_RS13825"
                     /old_locus_tag="pgaptmp_002765"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HGU4559250.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(14285..16045)
                     /gene="ycbZ"
                     /locus_tag="ACHOSM_RS13830"
                     /old_locus_tag="pgaptmp_002766"
     CDS             complement(14285..16045)
                     /gene="ycbZ"
                     /locus_tag="ACHOSM_RS13830"
                     /old_locus_tag="pgaptmp_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4559251.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDASQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            16231..16683
                     /gene="matP"
                     /locus_tag="ACHOSM_RS13835"
                     /old_locus_tag="pgaptmp_002767"
     CDS             16231..16683
                     /gene="matP"
                     /locus_tag="ACHOSM_RS13835"
                     /old_locus_tag="pgaptmp_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HGU4559252.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(16759..17799)
                     /gene="ompA"
                     /locus_tag="ACHOSM_RS13840"
                     /old_locus_tag="pgaptmp_002768"
     CDS             complement(16759..17799)
                     /gene="ompA"
                     /locus_tag="ACHOSM_RS13840"
                     /old_locus_tag="pgaptmp_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HGU4559253.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(18156..18665)
                     /gene="sulA"
                     /locus_tag="ACHOSM_RS13845"
                     /old_locus_tag="pgaptmp_002769"
     CDS             complement(18156..18665)
                     /gene="sulA"
                     /locus_tag="ACHOSM_RS13845"
                     /old_locus_tag="pgaptmp_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HGU4559254.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            18884..19513
                     /gene="sxy"
                     /locus_tag="ACHOSM_RS13850"
                     /old_locus_tag="pgaptmp_002770"
     CDS             18884..19513
                     /gene="sxy"
                     /locus_tag="ACHOSM_RS13850"
                     /old_locus_tag="pgaptmp_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HGU4559255.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNISFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(19476..21638)
                     /gene="yccS"
                     /locus_tag="ACHOSM_RS13855"
                     /old_locus_tag="pgaptmp_002771"
     CDS             complement(19476..21638)
                     /gene="yccS"
                     /locus_tag="ACHOSM_RS13855"
                     /old_locus_tag="pgaptmp_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HGU4559256.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(21648..22094)
                     /gene="yccF"
                     /locus_tag="ACHOSM_RS13860"
                     /old_locus_tag="pgaptmp_002772"
     CDS             complement(21648..22094)
                     /gene="yccF"
                     /locus_tag="ACHOSM_RS13860"
                     /old_locus_tag="pgaptmp_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HGU4559257.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            22217..24271
                     /gene="helD"
                     /locus_tag="ACHOSM_RS13865"
                     /old_locus_tag="pgaptmp_002773"
     CDS             22217..24271
                     /gene="helD"
                     /locus_tag="ACHOSM_RS13865"
                     /old_locus_tag="pgaptmp_002773"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HGU4559258.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(24303..24761)
                     /gene="mgsA"
                     /locus_tag="ACHOSM_RS13870"
                     /old_locus_tag="pgaptmp_002774"
     CDS             complement(24303..24761)
                     /gene="mgsA"
                     /locus_tag="ACHOSM_RS13870"
                     /old_locus_tag="pgaptmp_002774"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HGU4559259.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(24857..25519)
                     /gene="yccT"
                     /locus_tag="ACHOSM_RS13875"
                     /old_locus_tag="pgaptmp_002775"
     CDS             complement(24857..25519)
                     /gene="yccT"
                     /locus_tag="ACHOSM_RS13875"
                     /old_locus_tag="pgaptmp_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HGU4559260.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            25692..26105
                     /gene="yccU"
                     /locus_tag="ACHOSM_RS13880"
                     /old_locus_tag="pgaptmp_002776"
     CDS             25692..26105
                     /gene="yccU"
                     /locus_tag="ACHOSM_RS13880"
                     /old_locus_tag="pgaptmp_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGU4559261.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDSGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(26150..26467)
                     /gene="hspQ"
                     /locus_tag="ACHOSM_RS13885"
                     /old_locus_tag="pgaptmp_002777"
     CDS             complement(26150..26467)
                     /gene="hspQ"
                     /locus_tag="ACHOSM_RS13885"
                     /old_locus_tag="pgaptmp_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HGU4559262.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(26525..27715)
                     /gene="rlmI"
                     /locus_tag="ACHOSM_RS13890"
                     /old_locus_tag="pgaptmp_002778"
     CDS             complement(26525..27715)
                     /gene="rlmI"
                     /locus_tag="ACHOSM_RS13890"
                     /old_locus_tag="pgaptmp_002778"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HGU4559263.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            27810..28088
                     /gene="yccX"
                     /locus_tag="ACHOSM_RS13895"
                     /old_locus_tag="pgaptmp_002779"
     CDS             27810..28088
                     /gene="yccX"
                     /locus_tag="ACHOSM_RS13895"
                     /old_locus_tag="pgaptmp_002779"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGU4559264.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(28085..28414)
                     /gene="tusE"
                     /locus_tag="ACHOSM_RS13900"
                     /old_locus_tag="pgaptmp_002780"
     CDS             complement(28085..28414)
                     /gene="tusE"
                     /locus_tag="ACHOSM_RS13900"
                     /old_locus_tag="pgaptmp_002780"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HGU4559265.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(28505..29164)
                     /gene="yccA"
                     /locus_tag="ACHOSM_RS13905"
                     /old_locus_tag="pgaptmp_002781"
     CDS             complement(28505..29164)
                     /gene="yccA"
                     /locus_tag="ACHOSM_RS13905"
                     /old_locus_tag="pgaptmp_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HGU4559266.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
BASE COUNT         7503 a         7114 c         7754 g         7062 t
ORIGIN      
        1 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
       61 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
      121 gctacaacat acccgcaggt gattcctgca cgactacagg tacaccggtt ttagtgtatt
      181 acctgtctgg tactatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
      241 aggtcgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
      301 gggccagaac ggcagaactt gcaattccag tcaggtgtaa cacgggaaac gcggggttag
      361 ttaacgtcaa cctgagtctg acggcaacca cagaccccag ctatccccag gcgattaaga
      421 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gaacaacatt atttcccctg
      481 ctggtggaac attaccgctc tctattcctg atgatgcaga cagtatcgcg cgaatgaatg
      541 tctatccagt cagcacgaca ggtgtaccac cagaaaccgg gcgatttgaa gccacggcaa
      601 cggtgagaat aaattttgat taacgcgtga acgtatgaag aaaaaaacga tatttcagtg
      661 cgttatcttg ttctttagca ttcttaacat ccatgtcggg atggctgggc ctgaacaagt
      721 tagtatgcat atttatggga atgtggtcga tcagggctgt gatgtcgcca ccaaaagtgc
      781 attacaaaat attcatattg gtgattttaa tatcagtgat tttcaggctg cgaataccgt
      841 aagcactgct gctgatttga atatcgatat caccggttgt gccgctggta ttactggcgc
      901 ggacgtcctt tttagcggcg aggctgacac ccttgcgccg acactgctca aactaactga
      961 cacaggcgga agcggtggta tggcaacggg gattgccgtg caaattcttg atgcgcaaag
     1021 tcagcaagaa atcccgctca atcaggtcca gcctcttacg cccttaaaag ccggggataa
     1081 cacactcaaa tatcaacttc gttataagtc cacaaaggcg ggagcaacgg gcggtaatgc
     1141 gacggcggtt ctctattttg atctggttta ccagtgaagg agtgatgttg aaacggataa
     1201 tctggattct gttcttactg ggattaacgt ggggctgtga gctatttgcc catgatggca
     1261 cggtcaacat tagcggatcg tttcgccgta atacatgcgt gctggcacag gatagcaagc
     1321 aaatcaatgt gcagttaggc gatgtctcgc tgacacgttt ttctcatggg aattatggcc
     1381 cggagaaatc tttcatcatt aatttgcagg attgcggaac tgatgtcagc acggttgacg
     1441 tcaccttttc aggaaccccc gacggagtgc agagcgaaat gctgtccatt gaaagtggta
     1501 cggatgcagc cagcgggctg gcaattgcga ttctggatga tgcgaagata ttgattccgc
     1561 ttaaccaggc gagtaaagac tacagcctac atagcggtaa ggtgccatta actttttatg
     1621 cgcaattgcg acctgtcaat agcgatgtgc agtcgggtaa agtgaatgcc agcgcgacat
     1681 ttgtacttca ttatgactaa tacgtggaac agattggcgc tcttgatttt cgccgtttta
     1741 tcgctgctgg tggcgggtga attgcgggca ggagtggtgg tcggcggaac gcgatttatc
     1801 tttccggcag acagagaatc gatatctatt ttactgacta ataccagtca ggaatcctgg
     1861 cttattaaca gtaaaatcaa ccgcccaacg cgttgggccg ggggggaagc gtcgacagtg
     1921 ccagcaccat tactggccgc tccgccactt attctcctga agcccggtac tacaggcacg
     1981 ttgcgcttgc tgagaacgga aagcgacatc ttgcctgtgg atcgcgaaac gctatttgag
     2041 ttaagcattg ccagcgtgcc atccggcaac gttgaaaatc agagcgtaaa agtggcgatg
     2101 cgctcggtat ttaaactgtt ctggcgaccc gaaggtttgc cgggcgaccc gctggaagct
     2161 tatcaacaat tacgctggac acggaattcg cagggtgtac aactcactaa cccaacgcct
     2221 tactacatta acctgattca ggtgagcgta aatggtaaag cgttaagtaa tgcgggagtt
     2281 gtgctgccta aaagccagcg tcagacaagc tggtgtcagg caatcgcacc gtgtcatgtc
     2341 gcctggcggg cgataaatga ttacggcggg ttgagtgcaa agaaggagca aaatctgccc
     2401 tgaaacaggt tcggaaaacg tttgcgtttt ttttgccgca ggtcaattcc cttttggtcc
     2461 gaactcgcac ataatacgcc cccggtttgc acaccgggaa tccaggagag ttcatgtact
     2521 accccttcgt tcgtaaagcc cttttccagc tcgatccaga gcgcgctcat gagtttactt
     2581 ttcagcaatt acgccgtatt acaggaacgc cgtttgaagc actggtgcgg cagaaagtgc
     2641 ctgcgaaacc tgttaactgc atgggcctga cgtttaaaaa tccgcttggt ctggcagccg
     2701 gtcttgataa agacggggag tgcattgacg cgttaggcgc gatgggattt ggatcgatcg
     2761 agatcggtac cgtcacgcca cgtccacagc caggtaatga caagccgcgt ctctttcgtc
     2821 tggtagatgc cgaaggtttg atcaaccgta tgggctttaa taatcttggc gttgataacc
     2881 tcgtagagaa cgtaaaaaag gcccattatg acggcgtcct gggtattaac atcggcaaaa
     2941 ataaagatac gccagtggag cagggcaaag atgactatct gatttgtatg gaaaaaatct
     3001 atgcctatgc gggatatatc gccatcaata tttcatcgcc gaatacccca ggattacgca
     3061 cgctgcaata tggtgaagcg ctggatgatc tcttaaccgc gattaaaaat aagcaaaatg
     3121 atttgcaagc gatgcaccat aaatatgtgc cgatcgcagt gaagatcgcg ccggatcttt
     3181 ctgaagaaga attgatccag gttgccgata gtttagttcg ccataatatt gatggcgtta
     3241 ttgcaaccaa taccacactc gatcgttctc ttgttcaggg aatgaaaaat tgcgatcaaa
     3301 ccggtggctt aagtggtcgt ccgcttcagt taaaaagcac cgaaattatt cgccgcttgt
     3361 cactggaatt aaacggtcgc ttaccgatca tcggtgttgg cggcatcgac tcggttatcg
     3421 ctgcgcgtga aaagattgct gcgggtgcct cactggtgca aatttattct ggttttattt
     3481 ttaaaggtcc gccgctgatt aaagaaatcg ttacccatat ctaattattt cttcgaattc
     3541 ctctttataa gataatcagg gctttatttt cagccctggt tgttttatat tcatcgctgt
     3601 tgcttattta gacattttgt acttttattg ttgaggttat taagcgaagc gacaatggat
     3661 tgtgttgctg cggttttata gggtagggga gaggcagatg cgaattaaac cagacgataa
     3721 ctggcgttgg tattacgatg aagagcacga tcgaatgatg ctcgatttag ccaatggtat
     3781 gctatttcgc tcacgttttg cgcgcaagat gttgactcca gatgcctttt ctcccgcagg
     3841 cttttgcgtt gacgacgccg cgctctattt ctcttttgaa gaaaagtgcc gcgattttaa
     3901 tttatctaaa gaacaaaaag ccgaactggt gctgaatgca ctggtagcaa ttcgctatct
     3961 caaaccgcaa atgccgaaaa gctggcattt tgtttcccat ggtgaaatgt gggtgcccat
     4021 gccgggcgat gcggcctgcg tttggttgag cgatactcac gagcaggtca atttgctggt
     4081 cgttgaatct ggcgaaaatg ccgcactatg cctgctggca caaccctgcg ttgttattgc
     4141 gggtcgcgcg atgcagttgg gcgacgccat taaaatcatg aacgacaggc tgaaaccgca
     4201 ggttaatgtt gacagcttca gcctcgaaca ggcagtctaa cgcgccaact taagtgcagt
     4261 cttcggtaca cagctacagc aaagaatggt gccatcatcg cccattgctg attttttcag
     4321 cggcgtgact tcgccttcta aaagctgaac acggcaactt ccacaaatgc ccgcgcggca
     4381 agaataaggg atacgaattc cctgattttc taattgctcc agcaacacct gttggttatt
     4441 tccacgaaat gcctgtccct gccagtcaat atctacattt gcgtccggtt gttgcgtgat
     4501 gttggcggta tcatcagcgg cagctgcgcc gtaaattttt gccggagccg ttgccagaat
     4561 ttccacctca tcgccaacgc gaatcacgcc gctattacgg gcaattaaat tctgaccaaa
     4621 atcgacatcg ccgttatcct gggcagtgcg gaaagattgc aatgttttta atggttcgcc
     4681 tgccggatgt ttttgccctt tttctgggct gacggtggtg aaaatacagc ggctacaagg
     4741 tttaaccaca tcaaacacca catcaccaat gcgaatcact ttccagctat cttcttccca
     4801 ggctgacgcg ccggaaacca ccagattggg gcggaattgc tccattttta cactggccgg
     4861 acaacgttgt tggagatcac gtaacgaggc ttcgttagca agaaggtaag gatagccatc
     4921 agcaaatgac aggggtacag tgttgtggcg tttcacgcgc cgggtcattt gtggccccac
     4981 ccagcgtaat tgcacttcgc gggagaaaaa tccacttagc catttgttga tcgcgtctgg
     5041 cgcaattcgc gcggtaaaat gtgtgcccca aacttcggtt ggtgcgtctt gtgtggcgaa
     5101 agcagcaaaa cgaacatatg tgctactgcc atctggtgcg gtgagatgca agccatcatg
     5161 cacaggtgaa ggagtaaacc gtaccatctg gggaaactgg cgagcggtaa taaacgtacc
     5221 gtcaggttcc gtgatcataa agatgcgatc gaaggccaga ccactgacat cagccagagc
     5281 atgtgtaaga ccaatgccgc gcatcgattt aacaggatga ataaaaagcc gggttaatgt
     5341 cgccacggtg cggtcctcaa atgaaaataa gccctcaact ttatgacatg acgcgcttat
     5401 tagctataat gcgcaacaat tttcttagcg cctgtcccat taggctattt tatttgtcag
     5461 tttggccctg ggttgtgctc gaaatcctca cgtacttgtg tacgctccgg tttctccgcg
     5521 ctgtccatgt ccagactgac tgcaacaatt acgcctactg cggtaggctc ttagagtaaa
     5581 agtgacgata tgaattctct gtttgccagt acggcccgtg ggctggaaga gctgttaaaa
     5641 actgaactgg aaaacctggg ggccgttgaa tgccaggtgg ttcagggtgg ggtccatttc
     5701 aagggcgaca cacggcttgt ttaccagagc ctgatgtgga gccgcctggc ctcgcgtatt
     5761 atgttgccgc tgggcgagtg taaggtttac agcgatttag acctctatct cggtgttcag
     5821 gcgatcaact ggacagagat gtttaatcct ggcgcgacct tcgctgtcca cttcagtggt
     5881 ttgaatgaca ccatccgcaa cagtcagtac ggcgcgatga aagtgaaaga cgcgatcgtc
     5941 gatgctttca cgcggaaaaa tctgccgcgt ccaaatgttg atcgcgatgc gccggatatc
     6001 cgcgttaacg tctggctgca taaagaaacc gccagtatcg ctcttgatct gagtggtgat
     6061 ggtttacatc tgcgtggcta tcgcgatcgt gctggtattg cgccgatcaa agaaaccctg
     6121 gcagccgcta ttgtgatgcg atccggctgg cagccaggaa caccgctgct cgatccgatg
     6181 tgtggttccg gtacgttgct gattgaagcg gcgatgctgg cgaccgatcg cgcaccaggc
     6241 ttgcaccgtg ggcgttgggg ctttagcggc tgggcgcagc atgatgaagc tatctggcag
     6301 gaagtgaaag cggaagcgca aactcgcgcc cgtaaaggcc tggctgagta tagctctcat
     6361 ttttacggtt cggacagcga cgcacgggtg attcaacgtg cacgcactaa cgcccgtctt
     6421 gcggggattg gtgaactgat cacctttgag gtgaaagatg tcgcgcaact gaccaatccg
     6481 ctgccgaaag ggccgtacgg cacagtgttg agcaacccgc catacgggga acgtctggac
     6541 agcgaaccgg cgctgattgc gctgcatagc ctgctgggtc ggatcatgaa aaaccagttt
     6601 ggtggctgga atctctcttt gtttagtgcc tcgccggatc tgctaagctg cttgcagctg
     6661 cgtgcagaca aacagtacaa ggcgaaaaac ggcccgctgg actgcgtaca gaaaaattac
     6721 cacgttgccg aaagcactcc agacagcaaa ccggcgatgg tagcggaaga ctacgccaac
     6781 cgcctgcgta agaacctcaa aaaattcgag aagtgggctc gccaggaagg aattgaatgt
     6841 taccgcctgt atgacgccga tctaccggaa tataacgttg ccgttgaccg ttatgccgac
     6901 tgggtggtgg tgcaggagta tgcgccgcca aaaactattg atgctcacaa agcgcgtcag
     6961 cgtctgttcg atattatcgc tgcaaccatt tcggtactgg ggattgcgcc aaacaaactg
     7021 gtgctgaaaa cccgtgaacg ccagaagggc aaaaatcagt accagaaact gggcgagaag
     7081 ggcgagtttc ttgaagttac cgaatataac gctcacttgt tggtgaacct gacggattac
     7141 ctcgataccg gtctgtttct cgatcaccgt atcgcccgtc gtatgctcgg tcagatgagc
     7201 aaaggcaaag atttcctcaa cctgttctct tataccggca gcgccaccgt gcatgcggga
     7261 ttaggcggtg cacgcagcac caccaccgtg gatatgtcgc gtacttatct ggagtgggca
     7321 gaacgcaacc tgcgtttgaa tggcctgacc gggcgtgcgc atcgcctgat tcaggccgat
     7381 tgcctggcgt ggctgcgtga ggcaaatgaa cagttcgatc tgatctttat cgatccgcca
     7441 accttctcta actcaaaacg aatggaagat gcgtttgatg ttcagcgcga tcatctggcg
     7501 ctgatgaaag atttgaaacg tctgctgcgt gcaggtggga cgatcatgtt ctcgaacaat
     7561 aaacgtggct tccgtatgga tctcgacggc ctggcaaaac tgggactgaa agcacaagaa
     7621 attacgcaaa aaacgctctc ccaggatttc gcccgtaacc gccagatcca caactgctgg
     7681 ctgattaccg cagcctgaaa ggaatagtaa tgtcattaat cagtatgcat ggcgcatggc
     7741 tgtcgttcag cgacgcgccg cttctcgata acgcagaact gcatatcgaa gataacgaac
     7801 gtgtttgtct ggtgggccgc aacggcgcag gcaaatcgac gttaatgaaa atcctcaacc
     7861 gtgaacaagg gctggatgac ggtcgcatta tttacgagca agatttgatt gtagctcgtc
     7921 tgcaacagga tccgccgcgt aacgttgagg gtagcgttta tgatttcgtt gccgaaggca
     7981 ttgaagaaca agcggaatat ctgaaacgct atcacgatat ttcgcgcctg gtgatgaacg
     8041 acccgagcga gaaaaatctc aacgaactgg cgaaggttca ggaacagctg gatcaccaca
     8101 acctgtggca gctggaaaac cgcatcaacg aagtgctggc gcaactgggg ttagatccta
     8161 acgttgcgct gtcgtcgctt tccggcggct ggttgcgtaa agcggcatta ggacgcgcgc
     8221 tggtgagtaa tccgcgcatg ctgttgcttg atgaaccgac aaaccacctg gatattgaaa
     8281 ccatcgactg gctggaaggg tttttgaaaa ctttcaacgg gacgattatt ttcatctccc
     8341 acgaccgttc gtttatccgc aatatggcga cgcgcattgt tgatctcgat cgcggcaagc
     8401 tggtgaccta tccagggaat tacgaccagt acctgctgga aaaagaagaa gccctgcgcg
     8461 tggaagaatt acaaaatgcc gagttcgatc gcaaactggc gcaggaagag gtgtggatcc
     8521 gccaggggat caaagcacgc cgtacccgta atgaaggccg cgtacgcgcc ctgaaagcga
     8581 tgcgtcgcga gcgtggtgaa cgtcgcgaag tgatgggtac cgcaaagatg caggtggaag
     8641 aggccagccg ctccggtaag atcgttttcg aaatggaaga cgtttgctac caggttgacg
     8701 gtaagcaact ggtgaaagat ttttcggccc aggttctacg tggcgacaaa attgccctga
     8761 ttggtccgaa tgggtgcggc aaaaccacgc tgctgaaact gatgctcggt cagcttcaag
     8821 cggacagcgg gcgtattcac gttggcacca aactggaagt ggcttatttc gatcagcacc
     8881 gcgcggaact ggatcccgat aaaacggtga tggataacct tgccgaaggt aagcaagagg
     8941 tgatggttaa cggcaagcca cgccacgtat tgggctattt gcaggacttt ctgttccatc
     9001 cgaaacgggc gatgacgccg gtacgtgcgc tttctggcgg tgagcggaac cgcttgctgc
     9061 tggcgcgttt gttcctcaaa ccaagcaact tattgattct tgacgaaccg accaacgatc
     9121 ttgatgtcga aacgctggaa ctgctggaag aactgatcga cagctatcag ggcacggtat
     9181 tgctggttag ccacgatcgt caatttgtcg ataacaccgt tacagaatgt tggatcttcg
     9241 aaggcggcgg taaaattggt cgttatgtcg gcggttatca tgatgcccgt ggtcagcaag
     9301 agcagtatgt ggcgctcaaa cagcctgcgg tgaaaaaaac cgaagaagcc gccgcggcaa
     9361 aagcagaaac tgtaaaacgc agcagtagca aactaagcta taaattgcag cgcgaactgg
     9421 agcagctacc gcaattgctc gaagatctgg aggcgaagct ggaagcccta cagacgcagg
     9481 tggcggatgc ttccttcttc agtcagccac atgagcagac acaaaaagtg ctcgccgata
     9541 tggctgctgc agagcaggag ctggagcaag cctttgaacg ctgggagtat cttgaagcgt
     9601 taaaaaatgg tggctgatcg caaaatagtc gatttaaaca gagacagctg acaacggtaa
     9661 aattgcctga tgaactgggt ttatcaggca tgaaaaccaa cactttgtta gtcatctgta
     9721 aaatagcgca tcattaagga gtaccaatgt gcgaacatca tcatgccgcg aagcacatcc
     9781 tgtgctcgca gtgtgacatg ctggtggcgt taccgcgcct tgagcatggt cagaaagcgg
     9841 catgtccccg gtgtggcaca acgttaaccg tggcgtggga tgcccctcgg cagcgtccga
     9901 ccgcctatgc gttggctgca ctgttcatgc tgttgctgtc caacttgttt ccttttgtga
     9961 atatgaacgt tgcaggagtt accagtgaaa ttacattact ggaaattccc ggcgtgcttt
    10021 tttctgagga ctacgccagc ctcggcacct ttttcctgtt gtttgtgcaa ctggttcccg
    10081 cgttttgtct gataaccatt ctgttactgg tgaatcgcgc ggaattaccg gtccgtttaa
    10141 aagagcaact ggcacgggtg ctttttcaac tcaaaacctg gggaatggcg gagattttcc
    10201 tcgcgggtgt gctggtcagt ttcgttaaac tgatggctta cggcagcatt ggggtaggca
    10261 gcagctttct cccctggtgt ttattttgtg tcctgcaact gcgcgctttt cagtgcgttg
    10321 atcgtcgctg gttatgggac gacatcgccc cgatgccaga actgcgccag ccgctaaaac
    10381 caggcgtcac ggggatacgt caggggctgc gttcgtgctc ctgttgtacg gcaatccttc
    10441 ctgctgatga acccgtgtgc ccgcgttgta gtaccaaagg gtacgttcgg cgtagaaaca
    10501 gcctgcagtg gacactcgcg ctgcttgtaa cgtccatcat gctgtatctt ccggctaata
    10561 ttttgcccat catggtgacg gatttattag gctcgaagat gccgtcgacg attctcgctg
    10621 gggtcattct gttatggagc gaaggctctt atcccgtcgc tgcggtgatc tttctggcca
    10681 gtattatggt gccaacgtta aagatgatcg ccatcgcgtg gctgtgttgg gatgccaaag
    10741 ggcatggcaa gcgcgacagt gaaagaatgc atttgattta tgaagttgtt gagtttgtag
    10801 gccgctggtc gatgattgac gttttcgtta tcgcggtgct ctcggcgctg gtgcgtatgg
    10861 gaggtttaat gagtatttat ccggcaatgg gtgcattaat gtttgcttta gtcgtcataa
    10921 tgacaatgtt ttctgctatg acgtttgacc cgcgtttgtc gtgggatcgt caacctgaat
    10981 cagagcatga ggagtcctga cagtatggaa tctaataata gggaagccaa aatccagaaa
    11041 gtgaagaact ggtctcccgt gtggatattt cctatcgtca cggcgctcat tggggcctgg
    11101 gttctttttt atcattacag ccatcaggga ccggaagtga ccctgatcac cgcgaatgcg
    11161 gaaggaattg aaggtggcaa aaccaccatt aaaagccgta gcgttgacgt cggcgtggtt
    11221 gaaagcgcca cactggctga tgatttgacg cacgttgaaa tcaaagcgcg gctgaattcc
    11281 ggtatggaaa aattgctgca taaagatacc gtcttttggg tggtgaaacc gcagattggt
    11341 cgagaaggga ttagcggtct gggaaccctg ctgtctgggg tttatatcga actgcagcca
    11401 ggcgcgaaag gcagcaaaat ggataaatac gatttgctgg actcgccacc gttggccccg
    11461 cctgatgcga aaggtatccg tgtgattctc gatagcaaaa aagccgggca gctctcgcca
    11521 ggagatccgg tgctgttccg tggctatcgg gtaggttcgg ttgaaaccag caccttcgat
    11581 acacaaaaac gcaatatcag ctatcaactg ttcatcaatg caccttatga ccgactggtg
    11641 accaacaatg ttcgcttctg gaaagatagt ggcattgcgg ttgatctgac gtcagcaggg
    11701 atgcgtgtgg agatgggctc attgacaacg ctgctgagtg gcggtgtcag ctttgatgtg
    11761 ccggaaggtc tggatttagg gcagccagtg gcaccgaaaa cagctttcgt tttgtatgat
    11821 gatcagaaga gcattcagga ttcgttgtac accgatcaca ttgattatct gatgttcttt
    11881 aaagattcgg tacgcggtct gcaaccggga gctccggtag agttccgggg tattcgcctg
    11941 ggtaccgtaa gcaaagtgcc attctttgcg ccgaatatgc gtcagacatt taacgatgat
    12001 taccgtattc cggtactgat tcgtatcgag ccagagcggc tgaaaatgca gcttggcgaa
    12061 aatgcggatg ttgttgagca ccttggcgaa ttgttgaaac gtggtttacg cggatcgctg
    12121 aaaaccggaa acctggtcac tggtgcactg tatgttgatc tcgatttcta tccaaatacg
    12181 cctgcaataa ccggtattcg tgaatttaat ggttatcaga ttatcccgac cgttagcggc
    12241 ggcctggcgc aaatccagca acgactgatg gaagcgttgg ataagatcaa caaactgcca
    12301 ttgaatccga tgattgaaca ggcaaccagt acgctttctg aaagtcagcg cacaatgaaa
    12361 aacctgcaaa cgacgctgga tagcatgaac aagatcctcg ctagccagtc gatgcagcag
    12421 ttgccgacgg atatgcagtc aacgttgcgt gaattgaatc gcagcatgca gggcttccag
    12481 cctggctccg cagcctacaa caagatggtg gcggatatgc agcgccttga tcaggtgttg
    12541 cgagaactgc aaccggtgct gaaaacgctc aatgagaaga gtaacgcgct ggtatttgaa
    12601 gcgaaggaca aaaaagatcc agagccgaag agggcgaaac aatgaaaaag tggctagtga
    12661 cgattgcagc actgtggctg gccggatgca gctccggcga aattaataaa aactattacc
    12721 agttacctgt ggtgcagagc ggtacacaaa gtaccgccag ccagggcaat cgtctgttat
    12781 gggtagagca ggtcactgtt cctgactatc tggcggggaa tggtgtggtt tatcaaacca
    12841 gtgatgtgaa gtatgtgatt gccaacaaca acttgtgggc cagcccgttg gatcaacagt
    12901 tgcgcaacac cctggttgcc aacctgagta cgcaactgcc cggctgggtg gttgcctccc
    12961 agcctctggg aagcgcccag gacacgctca atgttaccgt aacggagttt aacggtcgct
    13021 atgatggcaa ggtcattgtc agtggtgagt ggctgttgaa ccaccaggga caactgatca
    13081 aacgtccgtt ccgtctggaa ggagtgcaaa ctcaggatgg ttacgatgag atggttaaag
    13141 tgctggccgg tgtctggagt caggaagccg cttctattgc acaagagata aagcgtctac
    13201 cttaattata aagatttgta aatataaccg tctccggtat gttgcctgag gcggtttttt
    13261 tgtttctaac gtgcggaaaa atttgttcct cttcacattt tttgtacaac cgacatgccc
    13321 gtgtagctca caaatatgac agtggcgtga attttgcgca ttgacggcag ttatgattcg
    13381 cggtattgct taactgtgat tgcacattta gtaatcactg ttttcttttc caccagaaac
    13441 cagtatgagg gaaacgaggc atgaagagac aaaaacgaga tcgcctggaa cgggcacatc
    13501 aacgtggtta tcaggccggc atcgccggac gctcaaaaga aatgtgtccc tatcagacgc
    13561 tgaatcaaag gtcacaatgg ctgggaggct ggcgagaagc catggcggac agggtagtaa
    13621 tggcctgatt ctgtctcttt aaaaagaaac ctccgcattg cggaggtttc gccttttgat
    13681 actctgtctg attataatca gaaggcagac gtatcctgga acagaccgac tttcaggtcg
    13741 ctggcggtat agatcagacg accatcaacc agcacttcgc catccgccag gcccataatc
    13801 agacgacggt taacaatgcg tttaaagtga atacggtagg tcactttttt cgctgtcggc
    13861 agtacctgac cagtgaattt cacttcgcca acgcccagcg cgcggccttt accttcgccg
    13921 cccagccagc cgaggtagaa ccctaccagc tgccacattg cgtccaggcc caggcatccc
    13981 ggcataaccg gatcgccaat aaagtggcat ccgaagaacc acagatccgg attgatatcc
    14041 agttctgctt caacataccc tttgtcgaag ttaccacccg tttcggtcat tttgaccaca
    14101 cggtccatca tcagcatgtt cggtgctggc aattgcgggc ctttagcgcc aaacagttca
    14161 ccgcgaccag aggcaagaag gtcttctttt gtataggatt cgcgtttatc taccatgttc
    14221 tctgtaagcc ttattttatt gaagcacgca ggatagctaa cacgtgtacg ctgaacaagt
    14281 ccgatcagtt cggaataaac cagttcagcc aacgtaatgg ccatggaaaa cggtgacgtc
    14341 cttcctgttg cgatgcttgc gcgatacgtt cctggatggt ttgcatcagc gtcgtttggc
    14401 cttcgccatc ccacaccaga tttaataata acggcagtgc gtcagtcaca tcgtctactg
    14461 cccagatggt gaatttgcct tcttctaccg ctttcaccag ttcactgtga agacttaaat
    14521 ggcgaacgtt agctgtgggg ataatgacac cttgtttccc ggttaactca cgttgctggc
    14581 aaatagcaaa gaagccttcg attttctcat ttaaaccacc gaccggctgg gcgcgaccga
    14641 actgatcgac tgaacctgtg atagcgatac tctgattcac cggcacatcg gcgagggcgc
    14701 ttatcagggc gcagagttca gccatcgagg cactatctcc atcaacttca ctgtatgact
    14761 gctcaaatgt cagcgatgct gagaagggga tctgttgctc aagctgtagt tccgacatca
    14821 ggaacgcttg catgatcatc atccctttcg catggatatt gccgccaagc tccgctttgc
    14881 gttcgatgtc ggtgaattca ccatcgccaa tatgcacaac gcagctaatg cgagaaggtt
    14941 cgccaaaagc gcgtggatga cccggaaatt caatgaccga aagggcgtta atttgcccga
    15001 tgcgttcgcc ttcggtttca atcaggattt gctcctgaag gatctcatcc tgcatacgtt
    15061 cagcgaggaa accttcgcgc cattcacgct gctgcagcat taagtttagc tgctcgccgg
    15121 agaaggtgtc gccatcacac agggaggcga cctctttaca ctggcggagg atccactgcg
    15181 ggctaagcgg aagtgtttct tgttcaccgg tgtagcgtgc tgcttcgcgg ataagtaccg
    15241 gccaggcatc cgctcccggt gcaggtaagt gattatgtct ggcggtaaat gtcacccagc
    15301 gacaccactg ggttactgac tccgcatcga caatctgcag agtatcttca aattcgctat
    15361 aaatagcctg ctctgaaagc tctggctcca tctcctggaa atcagccaat gattcgcgtt
    15421 cgcctaccag aatgactttc agcttcaatg gcatcgaagg cacagagacg gggagagggc
    15481 gcgactcatc aaacgcaacc cagtcaaaac gctcgcggtt aacgatattt ttcagccgca
    15541 tccacagcag aggttgcgcc agcagtgtac gcaaagagat gatgagaata ccgccatttg
    15601 cctgatgcac cagaccaggc tgcagggtaa tgtcgccatt aaactggcgc aggcagccaa
    15661 agagttgctc cgcttctacc cagtcggcag cgacaacttg cgttaaagtc gcaaaattat
    15721 catctgcact cactgcgtga cgtaagcgga tggagtggcc ggaaacctca tagtgaccgc
    15781 ccaccagttg gcttgcatcg ctttgtagcg tacgcgcggc attggcaata agattcagat
    15841 actcagactc ttccggggcc ttcgccagca taaaagagga ggatgctcgc gtatgcagca
    15901 actgctccag cgcaaattgc agtcgcggtt gagtatcact gaataaagga tcgttttcgt
    15961 caatcaaatg tggctgagca aatatttcct gatagctatc ggtatcagga accaggtcac
    16021 gccatgcaag tttcgtaatg gtcaaagttg atgtttttta gtctgttgtc aaagccgcga
    16081 ttataccgta accggcacta cagcacacgt agaaagcacc gacaatactc ccggcatggg
    16141 cgttaaagct cacaggatgg agattctttt cttcactggc ctaaaaagct gatattctgt
    16201 aaagagttac acggtaacat tgagatcgct atgaaatatc aacaacttga aaatcttgaa
    16261 agcggttgga aatggaagta tctggtgaag aagcaccgcg aaggggagtt aatcacccgt
    16321 tacatagaag ccagtgccgc ccaggaagcc gtcgatgtgt tgctctcgct ggaaaatgaa
    16381 cccgtgctgg taaatggctg gattgacaag catatgaatc cggaactggt caatcgcatg
    16441 aagcaaacca ttcgggccag acggaagcgc cattttaatg cggaacacca gcatacgcgc
    16501 aaaaaatcga tcgatctgga atttattgtc tggcaacgtc tggctggtct tgcgcagcgt
    16561 cgcggtaaaa cgctttctga aacgattgtt cagctgattg aagatgcgga aaacaaagag
    16621 aaatacgcga ataaaatgtc ttctctgaag caggatctgc aggcattgct gggtaaggaa
    16681 taactgacga aagtcagttc aatttactaa aggcaaaaaa aaccccgcag cagcggggtt
    16741 tttctaccag acgagaactt aagcctgcgg ctgagttaca acgtctttga tacctttaac
    16801 ttcgatctct acgcgacgat ccggagccag gcagtcgatc agtgcagcac gctgtttcac
    16861 gttgtcacag gtgttgccag taaccgggtt ggattcgccc ataccacgtg cggagatctt
    16921 gtctgccggg atacctttgg agatcaggta atcaacaaca gactgagcac ggcgctcgga
    16981 cagaccctgg ttgtaagcgt cagaaccgat gcggtcggtg taacccagaa caactacgga
    17041 accgtctttc ggatccaggt tgctcagctg gctgtacagc tgatccagag cagcctgacc
    17101 ttccggtttc agggttgctt tgttgaagtt gaacagaacg tcagacttca gagtgaagtg
    17161 cttggtctgt acttccggtg ccggagctgg agccggagca actactggag ctgcttcgcc
    17221 ctgaccgaaa cggtaggaaa cacccaggct cagcatgccg ttgtccggac gagtgccgat
    17281 ggtgtgtgcg tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg
    17341 agtgatcgcg tactcaacac cgccagcgaa gaccggagaa acgccggtgt cgtggttttt
    17401 accataaacg ttggatttag tgtctgcacg ccataccata ccacccagac gagtgtagat
    17461 gtccaggtcg tcagtgattg ggtaacccag tttagcggtc agttgaacgc cctgagcttt
    17521 gtatgcaccg ttttcaacgc tgcctttgta cggcatacga cctaaccagt cgtaacccat
    17581 ttcaaagcca acatacgggt taacctggta accaccaaaa gcaccagcgc ccagttggtt
    17641 ttcatgggtc gggccattgt tgttgatgaa accagtgtca tggtactggg accagcccag
    17701 tttagcacca gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc
    17761 agccagtgcc actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc
    17821 caaaatacgc catgaatatc tccaacgaga taacacggtt aaatccttca ccgggggatc
    17881 tgctcaatat taactctacc gatatcttcg gcttatgccg agcacccctg gcgatgtaaa
    17941 gtctacaacg tagttgaaaa cttacaagtg tgaactccgt caggcatatg aaaaaaaagt
    18001 cttgtataag gtatgtttaa tcttttttgt cagcgacaat ttacagaaga aaatcgcgga
    18061 aaccgcttca gacaagcctc cgcaaggaaa attagtcacg actgaaagca ttggctgggc
    18121 gacaaaaaaa gttccaggat taatcctaaa tttacttaat gatacaaatt agagtgaatt
    18181 tttagcccgg aaagttgtct cgtggcgtga gaggatgcgc ttaccggacg cataataaac
    18241 cccatagcgt taccttcatt tgccgcatca acaagttcag catgctcttc ttcagtcaaa
    18301 tcatctgcca accaaccgat caccacactg taattgcccg tgcgtaaagc gcgaaccatt
    18361 gactccacag tgtggcaagg ggagagctgg ctaatctgca ttactttcgt taagggtagc
    18421 ccagatgcct gaacccattc ccgactcagt ttttgttgcg gtgttaacca gagttgccag
    18481 cgcgattgct gaccgagttg ctgtaacaat ggcaacagta gaagttgcgt catcatgggc
    18541 tgatcttcgc gatagacaac ttcactgata agcccggctg tagtgttttc cgtagagaca
    18601 cgcgcaattt tacttgctgc ggatgagaac gacgaagaac gatgtgcata gcctgaagtg
    18661 tacataatca atccagcccc tgtgagttac tgtatggatg tacagtacat ccagtgacaa
    18721 caaagatcaa ccctattttc ggaaagagcc tcgcaaattt tgtcgttggt gacgggaaaa
    18781 cataaattaa tcttgcccct taagaataag ttgcctattt tcgtagttaa cggatccgtt
    18841 aatgtgaatc attcttttat gttatgattt taaaaggaat tttatgaaaa gcctctccta
    18901 taagcggatc tataaatcac aagaatacct ggcaacgttg ggcacaattg aataccgatc
    18961 attgtttggc agttacagcc tgaccgttga cgacacggtg tttgcgatgg tttctgatgg
    19021 tgagttgtat cttcgggctt gtgagcaaag tgcacagtac tgtgtaaaac atccgcctgt
    19081 ctggctgaca tataaaaagt gtggccgatc cgttaccctc aattactatc gggttgatga
    19141 aagtctatgg cgaaatcaac tgaagctggt gcgtctgtcg aaatattctc tcgatgcagc
    19201 gctgaaagag aaaagcacgc gcaatacccg ggaaagactg aaagatttgc ccaatatatc
    19261 ttttcatctg gaagcgattc tcggggaggt ggggattaag gatgtacggg cgttacgtat
    19321 acttggggca aaaatgtgtt ggttgcgact gcggcagcaa aacagtctgg tgacagaaaa
    19381 gattctgttt atgcttgaag gtgccattat cggcattcat gaagctgcgc tcccggtggc
    19441 acgccgccag gagcttgcag aatgggctga ctctcttacg ccgaaacagg agtttcctgc
    19501 ggaacttgag taatctggcg ttgcagacga ccaatctcag gcagtaatgc aatcaataat
    19561 ccaacttgtt gtacgaccag aggttctttg ctgtctgccc gtggttcaag ttgctgcatc
    19621 cgctgtttca ggctagctaa tgcctcattg acgcgttctt catcagcagg ttgatgatgt
    19681 aacgcgtcat caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt
    19741 aactgctccc ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac
    19801 agcaaccgaa acgcggcttc gcgaatttgc ggggtaacgt tcggctcgct ggacatattt
    19861 gataccaccg acgccagctc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac
    19921 gccagacggt tatcacgccc ctgatggtat tgctccagta tggcatcgag ataccgacag
    19981 ttggcctctg tggcgcgttc gagcatgcgc ggcagattgc gaaactgcca gtcaggccag
    20041 atgtagctca ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt
    20101 aacgctactt caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg
    20161 aacatcgttg catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata
    20221 accagcagca ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatg
    20281 gcgataccta ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag
    20341 tttggctggc agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt
    20401 atctgaatga tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag
    20461 agggcggatt ccggcgtgaa gtgacggcta agacgcagcc agatatcact caacccgtgc
    20521 gggctgtcat cagcgagctc attttcgtca ttattatggg gtagtgcctg ggcctgttct
    20581 gattcaattg ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt
    20641 gttttgagta aatcggcggg tgcgccgtta tcgcgcatcc gctccagcgc agcatcaata
    20701 tgcgtaaaag cgcgctcaaa atgcggatca tgttgataag gctgacgcaa caaaatacag
    20761 cgtgacagtt gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc
    20821 acgtcgctgt ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca
    20881 cgctcgtgaa tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga
    20941 ccacgatcgc cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc
    21001 agcagaccgt tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca
    21061 ggatcaaaca tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc
    21121 aggttgtcct gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg
    21181 taccagacgg caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat
    21241 gttcccaaca tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt
    21301 tgacccagac cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat
    21361 agccagggcc agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc
    21421 gtaatgatga ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct
    21481 gccaccatcc ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac
    21541 gctgtggttc cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag
    21601 gtatagcgtt tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga
    21661 cgacgtgcat tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca
    21721 accggccata gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg
    21781 attgaaatac attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag
    21841 aaaatcagcc agaaaatatt caataccgta ccgccagtat tcagcagcac atttttgcca
    21901 gccgggttca gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta
    21961 gtgatctccc agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga
    22021 gtcgccaaca gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc
    22081 aaaacggtac gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa
    22141 cgtttttttg ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt
    22201 gtggatctac agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca
    22261 cccttacgat cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga
    22321 ataccttatt cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg
    22381 cgagctggaa tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca ccgaatgggg
    22441 cgagacgcag cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat
    22501 gagcgaaatt gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg
    22561 agaaaataaa tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc
    22621 tttgtcggcg ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc
    22681 gtggcgtaaa tgtcaggcct ggttgaaaga tattgaaagc gctcggttgc agcataacca
    22741 ggcgtatacc gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc
    22801 accgctgaat ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct
    22861 ggcaggtgca ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc
    22921 gcgtggtgaa gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga
    22981 agagatggac gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt
    23041 tcatgcgctg gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa
    23101 actggaaaat gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg
    23161 cagcgaaaag aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg
    23221 gtcagtgcca gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg
    23281 cctcgatcgc tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc
    23341 cagtgcaccc gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt
    23401 attaaaagcc tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat
    23461 tcatcaggcg attgtgattc tggagaaagg tcgctttatc agcccgtgga agcatattct
    23521 ggttgatgaa tttcaggata tctcgccgca gcgggcagcg ttgttagcgg cattacgcaa
    23581 gcaaaacagt cagacgacgt tgttcgctgt tggtgatgac tggcaggcga tttaccgatt
    23641 cagcggtgcg caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgaacg
    23701 ttgtgattta gacacgactt accgttttaa cagtcgtatc ggtaaggtgg caaaccgttt
    23761 tattcagcag aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa
    23821 aaaagccgtc acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg
    23881 ttatgccaaa ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc
    23941 cagcctggaa aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca
    24001 tgccagcaaa gggcaacagg cggattacgt catcatcgtt ggcttgcagg agggaagtga
    24061 tggttttccg gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga
    24121 ggatttcccg gacgctgaag aacggcggtt aatgtacgtg gcgctgaccc gggcacgcca
    24181 tcgggtatgg gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa
    24241 tctggatgtg ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa
    24301 tattacttca gacggtccgc gagataacgc tgataatcgg ggatcagaat atcgaccgcg
    24361 tcgttgaaat gcggcgactg gattatgaag tctgccgttg ccacgttggt ggcgaccgga
    24421 atgttccata ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca
    24481 tttagtggat cccagaagaa aatcaataca tcaattttcc cttctgagat caatgcgcca
    24541 acctgctggt caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg
    24601 cgggaaatta agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt
    24661 tgatgccgtt ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca
    24721 atatgtttcc gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa
    24781 ctttcctaca gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg
    24841 cacaggcaca aaaatctcag gaagatggtt gtttttccgc ccactgcagg aaagtatttc
    24901 gcgtttgtgg gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag
    24961 actgcggtgg aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca
    25021 tcatcgtggc aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct
    25081 cgacctcata atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg
    25141 gaatcggcgt cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag
    25201 cttcccgctc attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat
    25261 taaaactgac caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa
    25321 tcgtcttctc aacgcgaaac actaactgat gcggtccatt atccagttca atgctgtccg
    25381 caccgcgcag cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg
    25441 ttgaaagccg cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg
    25501 tcacgatgcc ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatatgtc
    25561 aaaatttttc tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat
    25621 tcattcgtat tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc
    25681 tggaggaaat aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg
    25741 cgctggtggg ggcaagcgac aaacccgatc gtcccagcta tcgcgtgatg aaatatctac
    25801 tcgaccaggg gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg
    25861 gccagcaggg gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt
    25921 tccgtaattc agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa
    25981 ccctctggat gcaactgggg gtgattaatg agcaagcggc agtactggca cgggattccg
    26041 ggttaaatgt ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggcctggcca
    26101 aataaaaaat ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca
    26161 gacgcggggc ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt
    26221 caggatgttc atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg
    26281 gtaggccgtt atcgtcctcc atcactacgt gataccacgg agcggcgcga agctcgtcat
    26341 ttaccgccag ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga
    26401 ccactccgag ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg
    26461 caatcatagt cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt
    26521 caagttacat gacgcgacag gcaaaccctt tcagatatag cccttccgga taggtagcga
    26581 tcaccggatg atcggctgcc tgacggaact gctctataaa ttgtacatca cggccggcat
    26641 caattgcggc atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg
    26701 agaaagtcag gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatatctt
    26761 tatagccacg acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca
    26821 tcacgataac gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat
    26881 catcacggac aaactcagcc ttgctcagat ccagtttgtt tagctcaacg ttctgccgtg
    26941 caatatccag cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca
    27001 gtgccgatac ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa
    27061 cgtagcggcg ggtagccagg cggctgtctc gctggtccag gtagtagccc gttttgtgtc
    27121 cgtgctgaat atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg
    27181 gcaactcgcc ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa
    27241 catcgctgcg atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg
    27301 ccgcgcgctg atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac
    27361 gatcgatagt aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga
    27421 ggccatcttt ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa
    27481 aaaaagcaat gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg
    27541 aagctggtga ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg
    27601 tttcaccgag gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat
    27661 ggcgacgaag taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt
    27721 acttttctgt cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa
    27781 cgcgtccatt aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctgggttt
    27841 acgggcgagt tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg
    27901 gtttaaccgg gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcttgcggtg
    27961 aagaagggca ggtggaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc
    28021 gtgtagaacg ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta
    28081 tccgttaaat acattttacc ggttttggca ggccagcaat tttggtggct tgcttagctg
    28141 gacctttcgg aaacagtcgg tataaatagc ggctgttacc tttttcttcg ccaaatttat
    28201 tcgccatcgc ttttaccagc atacgaatcg ccggagaagt attgaattcc agatagaaat
    28261 cacgcacaaa acgcaccact tcccagtgtt ccggtgacag cgaaatccct tcgttctctg
    28321 caatcaccac tgccagcggc tcactccact ggctgctttc tttgagatag ccttcggtat
    28381 ccgtttctat ctctttacct tcgaagatca gcataattat tactacctta atcagactgg
    28441 cggcagtgta acaaaaaaca aagccccgca taaagcgagg ctatggaagt gttagcgggt
    28501 gagattaatc gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca
    28561 gcgaaacata caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaacgatgt
    28621 tgctggtttc aaacaaaata gcgccagagg agatcaaaat gaagaccgcg ctgatcgcca
    28681 gatgcagagc aggcagctgc aggaagatat tagcaaccat accaatcagc accaccacaa
    28741 tacccgccat cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg
    28801 cagagcagca gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca
    28861 ttccggcaga cagataggtg ttcagaatag gtccgaggat ataacccaga aaaccggtaa
    28921 aggcgaatgc ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca
    28981 aaccatacat acccaccagc gtcagaatca gacccggaga tggcagcatc agcaccgtac
    29041 tggcagttgc ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac
    29101 gcagcacctt atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat
    29161 ccattgagtc actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa
    29221 gggcacccag aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga
    29281 ttatcaggtc attaagcaaa tataacgccc tgagaatttc gacaggcaaa agaaaaaggg
    29341 gttagcattt agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga
    29401 accctttcgg gtcgccggtt ttcaagaccg aag
//
LOCUS       DAZKHC020000055        28995 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZKHC020000055 DAZKHC020000000
VERSION     DAZKHC020000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.055"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            224..1846
                     /gene="yhiJ"
                     /locus_tag="ACHOSM_RS13910"
                     /old_locus_tag="pgaptmp_002782"
     CDS             224..1846
                     /gene="yhiJ"
                     /locus_tag="ACHOSM_RS13910"
                     /old_locus_tag="pgaptmp_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4559267.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSKNYGCSLDNTDVNWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETIINPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GYLGIMTEGENFLCQLLTIEDEVLNVLLRENFNEQLDTNINTIISNLNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHYAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2036..2149
                     /gene="yhiY"
                     /locus_tag="ACHOSM_RS13915"
                     /old_locus_tag="pgaptmp_002783"
     CDS             2036..2149
                     /gene="yhiY"
                     /locus_tag="ACHOSM_RS13915"
                     /old_locus_tag="pgaptmp_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HGU4559268.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2212..3279
                     /gene="yhiI"
                     /locus_tag="ACHOSM_RS13920"
                     /old_locus_tag="pgaptmp_002784"
     CDS             2212..3279
                     /gene="yhiI"
                     /locus_tag="ACHOSM_RS13920"
                     /old_locus_tag="pgaptmp_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4559269.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3276..6011
                     /gene="rbbA"
                     /locus_tag="ACHOSM_RS13925"
                     /old_locus_tag="pgaptmp_002785"
     CDS             3276..6011
                     /gene="rbbA"
                     /locus_tag="ACHOSM_RS13925"
                     /old_locus_tag="pgaptmp_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HGU4559270.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVNENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYKPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPAIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQRPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6011..7135
                     /gene="yhhJ"
                     /locus_tag="ACHOSM_RS13930"
                     /old_locus_tag="pgaptmp_002786"
     CDS             6011..7135
                     /gene="yhhJ"
                     /locus_tag="ACHOSM_RS13930"
                     /old_locus_tag="pgaptmp_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4559271.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGSGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            7208..7483
                     /locus_tag="ACHOSM_RS13935"
                     /old_locus_tag="pgaptmp_002787"
     CDS             7208..7483
                     /locus_tag="ACHOSM_RS13935"
                     /old_locus_tag="pgaptmp_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGU4559272.1"
                     /translation="MRTKLQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            7480..7839
                     /locus_tag="ACHOSM_RS13940"
                     /old_locus_tag="pgaptmp_002788"
     CDS             7480..7839
                     /locus_tag="ACHOSM_RS13940"
                     /old_locus_tag="pgaptmp_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGU4559273.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7959..8360)
                     /gene="nikR"
                     /locus_tag="ACHOSM_RS13945"
                     /old_locus_tag="pgaptmp_002789"
     CDS             complement(7959..8360)
                     /gene="nikR"
                     /locus_tag="ACHOSM_RS13945"
                     /old_locus_tag="pgaptmp_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HGU4559274.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(8366..9172)
                     /gene="nikE"
                     /locus_tag="ACHOSM_RS13950"
                     /old_locus_tag="pgaptmp_002790"
     CDS             complement(8366..9172)
                     /gene="nikE"
                     /locus_tag="ACHOSM_RS13950"
                     /old_locus_tag="pgaptmp_002790"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HGU4559275.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(9169..9933)
                     /gene="nikD"
                     /locus_tag="ACHOSM_RS13955"
                     /old_locus_tag="pgaptmp_002791"
     CDS             complement(9169..9933)
                     /gene="nikD"
                     /locus_tag="ACHOSM_RS13955"
                     /old_locus_tag="pgaptmp_002791"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HGU4559276.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETYLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(9933..10766)
                     /gene="nikC"
                     /locus_tag="ACHOSM_RS13960"
                     /old_locus_tag="pgaptmp_002792"
     CDS             complement(9933..10766)
                     /gene="nikC"
                     /locus_tag="ACHOSM_RS13960"
                     /old_locus_tag="pgaptmp_002792"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HGU4559277.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(10763..11707)
                     /gene="nikB"
                     /locus_tag="ACHOSM_RS13965"
                     /old_locus_tag="pgaptmp_002793"
     CDS             complement(10763..11707)
                     /gene="nikB"
                     /locus_tag="ACHOSM_RS13965"
                     /old_locus_tag="pgaptmp_002793"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HGU4559278.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(11707..13281)
                     /gene="nikA"
                     /locus_tag="ACHOSM_RS13970"
                     /old_locus_tag="pgaptmp_002794"
     CDS             complement(11707..13281)
                     /gene="nikA"
                     /locus_tag="ACHOSM_RS13970"
                     /old_locus_tag="pgaptmp_002794"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4559279.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(13392..13979)
                     /gene="acpT"
                     /locus_tag="ACHOSM_RS13975"
                     /old_locus_tag="pgaptmp_002795"
     CDS             complement(13392..13979)
                     /gene="acpT"
                     /locus_tag="ACHOSM_RS13975"
                     /old_locus_tag="pgaptmp_002795"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HGU4559280.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(14034..15083)
                     /gene="yhhT"
                     /locus_tag="ACHOSM_RS13980"
                     /old_locus_tag="pgaptmp_002796"
     CDS             complement(14034..15083)
                     /gene="yhhT"
                     /locus_tag="ACHOSM_RS13980"
                     /old_locus_tag="pgaptmp_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU4559281.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFVRRGVKRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVILLVMTVVF
                     MLFEVRHVPYKMRFALNKPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            15214..16431
                     /gene="yhhS"
                     /locus_tag="ACHOSM_RS13985"
                     /old_locus_tag="pgaptmp_002797"
     CDS             15214..16431
                     /gene="yhhS"
                     /locus_tag="ACHOSM_RS13985"
                     /old_locus_tag="pgaptmp_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4559282.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRQTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(16435..16992)
                     /gene="dcrB"
                     /locus_tag="ACHOSM_RS13990"
                     /old_locus_tag="pgaptmp_002798"
     CDS             complement(16435..16992)
                     /gene="dcrB"
                     /locus_tag="ACHOSM_RS13990"
                     /old_locus_tag="pgaptmp_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HGU4559283.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(17065..17730)
                     /gene="yhhQ"
                     /locus_tag="ACHOSM_RS13995"
                     /old_locus_tag="pgaptmp_002799"
     CDS             complement(17065..17730)
                     /gene="yhhQ"
                     /locus_tag="ACHOSM_RS13995"
                     /old_locus_tag="pgaptmp_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HGU4559284.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            17951..18196
                     /gene="tusA"
                     /locus_tag="ACHOSM_RS14000"
                     /old_locus_tag="pgaptmp_002800"
     CDS             17951..18196
                     /gene="tusA"
                     /locus_tag="ACHOSM_RS14000"
                     /old_locus_tag="pgaptmp_002800"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HGU4559285.1"
                     /translation="MTALFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(18298..20496)
                     /gene="zntA"
                     /locus_tag="ACHOSM_RS14005"
                     /old_locus_tag="pgaptmp_002801"
     CDS             complement(18298..20496)
                     /gene="zntA"
                     /locus_tag="ACHOSM_RS14005"
                     /old_locus_tag="pgaptmp_002801"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HGU4559286.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQASRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(20570..21196)
                     /gene="yhhN"
                     /locus_tag="ACHOSM_RS14010"
                     /old_locus_tag="pgaptmp_002802"
     CDS             complement(20570..21196)
                     /gene="yhhN"
                     /locus_tag="ACHOSM_RS14010"
                     /old_locus_tag="pgaptmp_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HGU4559287.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            21337..21696
                     /gene="yhhM"
                     /locus_tag="ACHOSM_RS14015"
                     /old_locus_tag="pgaptmp_002803"
     CDS             21337..21696
                     /gene="yhhM"
                     /locus_tag="ACHOSM_RS14015"
                     /old_locus_tag="pgaptmp_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HGU4559288.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLEAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(21699..21968)
                     /gene="yhhL"
                     /locus_tag="ACHOSM_RS14020"
                     /old_locus_tag="pgaptmp_002804"
     CDS             complement(21699..21968)
                     /gene="yhhL"
                     /locus_tag="ACHOSM_RS14020"
                     /old_locus_tag="pgaptmp_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HGU4559289.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(21958..22554)
                     /gene="rsmD"
                     /locus_tag="ACHOSM_RS14025"
                     /old_locus_tag="pgaptmp_002805"
     CDS             complement(21958..22554)
                     /gene="rsmD"
                     /locus_tag="ACHOSM_RS14025"
                     /old_locus_tag="pgaptmp_002805"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HGU4559290.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            22704..24197
                     /gene="ftsY"
                     /locus_tag="ACHOSM_RS14030"
                     /old_locus_tag="pgaptmp_002806"
     CDS             22704..24197
                     /gene="ftsY"
                     /locus_tag="ACHOSM_RS14030"
                     /old_locus_tag="pgaptmp_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGU4559291.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            24200..24868
                     /gene="ftsE"
                     /locus_tag="ACHOSM_RS14035"
                     /old_locus_tag="pgaptmp_002807"
     CDS             24200..24868
                     /gene="ftsE"
                     /locus_tag="ACHOSM_RS14035"
                     /old_locus_tag="pgaptmp_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGU4559292.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            24861..25919
                     /gene="ftsX"
                     /locus_tag="ACHOSM_RS14040"
                     /old_locus_tag="pgaptmp_002808"
     CDS             24861..25919
                     /gene="ftsX"
                     /locus_tag="ACHOSM_RS14040"
                     /old_locus_tag="pgaptmp_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGU4559293.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            26164..27018
                     /gene="rpoH"
                     /locus_tag="ACHOSM_RS14045"
                     /old_locus_tag="pgaptmp_002809"
     CDS             26164..27018
                     /gene="rpoH"
                     /locus_tag="ACHOSM_RS14045"
                     /old_locus_tag="pgaptmp_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HGU4559294.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            27289..28392
                     /gene="livJ"
                     /locus_tag="ACHOSM_RS14050"
                     /old_locus_tag="pgaptmp_002810"
     CDS             27289..28392
                     /gene="livJ"
                     /locus_tag="ACHOSM_RS14050"
                     /old_locus_tag="pgaptmp_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HGU4559295.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(28591..28974)
                     /gene="panM"
                     /locus_tag="ACHOSM_RS14055"
                     /old_locus_tag="pgaptmp_002811"
     CDS             complement(28591..28974)
                     /gene="panM"
                     /locus_tag="ACHOSM_RS14055"
                     /old_locus_tag="pgaptmp_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HGU4559296.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPAVSCWWMADAGL
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6961 a         7420 c         7981 g         6633 t
ORIGIN      
        1 cagtatttca tagattttct taatcgattg tagaggcatg ttctgatgac gcctctacaa
       61 tctttaggga ctttctctgc tatgagataa gaaatttcat attatgcttt aaggctgaat
      121 gatttgtgca aaatataatt atttttccat cgaacatgag gtgtatgtca taagattaaa
      181 tgttaactat gtattcctcg cggtgaatta tatgaggtaa aagatgaaaa ttggaactgt
      241 ggcaggcact aacgacagta caacgacaat cgcaacaaat gatatggtgc aagagcatgt
      301 taccaacttt actaaagaac tgtttgggta tattgcaaat ggtataggtg atgatatatc
      361 aagtattgcc aggacaatgc tcggtgaggt tgtggaaaaa atagatgatt ggcaaataga
      421 acggttccaa caatctattc aagatgacaa aatttcattt accatacaaa cggatcattc
      481 agaaaaatat agtatgctat cgggcatgcg tgctcatatt ctacgtaggg ataataatta
      541 tcagtttatt gtgacaatta atagtaaaaa ttacggttgc tctcttgata atactgatgt
      601 taattggtgc agtattgttt atttacttaa taatatgaca gtaaatgata atgctaatga
      661 tgttgcagtt actgagtcgt acaaacccat ctggaactgg aaaatatcgc aatacaatgt
      721 atttgatatt aaattcgaaa caattataaa cccccagttc gctgacagaa tctatttctc
      781 caattgttca cctgttgatc ccacaagtac caggccgacc tattttgggg ataccgatgg
      841 ctcagttggt gcagttttgt acgcgctttt tgctacggga tatttgggaa tcatgactga
      901 aggagaaaat ttcctttgcc agttgcttac tatagaagat gaagttctca acgtattgct
      961 aagggaaaac tttaatgagc aacttgacac taatattaat acaataatca gtaacctgaa
     1021 cagaagagat aatgttttag aaagtcttca gccttattta gttattaata aagacgcagt
     1081 cacgccatgt acgtttctgg gcgaccaaac cggcgatcga tttagtaata tctgtggcga
     1141 tcaatttatc atcgatttgt taaaacgcat aatgagtatc aatgaaaacg ttcatgtatt
     1201 agctggaaat catgaaacga attgcaatgg taactatatg cagaacttca cgcgcatgaa
     1261 accacttgat gaggatacct actctgggat aaaagattat cctgtatgtt tctatgaccc
     1321 caaatacaaa ataatggcaa atcatcatgg gataactttt gatgatcagc gaaaacgtta
     1381 cattatagga ccgataacag tctctagtga tgaaatgacc aatgctctcg atccggtcga
     1441 attagcggca attatcaata aaaagcacta tgctgtaatt aatggcaaaa agtttaaaaa
     1501 tagccgggct atctcttgtc gatcctttaa tcgttatttt tctgtttcga cagattacag
     1561 acctaaactt gaagcactat tagcatgctc acaaatgtta ggtataaatc aagttgttgc
     1621 acataacggt aatggtggaa gagaatgtat aggtgaaacg gggacagttt taggtcttaa
     1681 cgcccgtgac agtaaacatg ctggaagaat gttcagcatg cataattgcc aaattaaccc
     1741 tggtgcaggg cctgagataa ccactccctg gaagtcttac cagcatgaaa aaaacagaaa
     1801 tggacttatg cctttgatca gaaggcgcac aatgttacag ctctgaacca tagacaacat
     1861 gaatattaat gttactatct ggttgagact gttgataaaa cgtaaaaagg aatgctctgt
     1921 tcctgcaact gaacaggcgt gaaacgcgat ccaacaggat ggataccgtt ttgtcagcca
     1981 gttttataga tcacacttat gagtatgcgt gtttcattta cggccaggaa tgctgatgat
     2041 gacaacatta ctacccgttt tcactaagcc ttccccgttg gcgctcaatg ctctgcgcgc
     2101 tggtcgtatt tgtcgtttcc ttcttatccc ggatggaaga atccgctgaa cgtcgttcgg
     2161 cggcagttgt aacacgcgtc gtttgcttac agggattatg gcgggccgat tatggataag
     2221 agtaagcgcc atctggcgtg gtgggttgtc ggggcactgg cggtggctgc cgtcgtggcg
     2281 tggtggctgt tgcgcccggc gggtgtgccg gaaggctttg ctgtcagcaa tgggcgcatt
     2341 gaagcgacgg aagtggatat cgccagcaaa attgccgggc gtatcgacac cattctggtg
     2401 aaagaaggcc agtttgttcg cgaaggtgaa gtgctggcga agatggatac tcgcgtgttg
     2461 caggaacagc gactggaagc catcgcgcaa atcaaagagg cacaaagcgc cgttgccgcc
     2521 gcgcaggctt tgctggagca acgacaaagc gaaactcgcg ccgcgcagtc gctggttaat
     2581 caacgccagg cagaactgga ctccgtagca aaacgtcata tgcgttcccg ttcactggcc
     2641 caacgagggg ctatttctgc gcaacagctg gatgacgacc gcgccgccgc tgagagcgcc
     2701 cgagctgcgc tggaatcggc aaaagctcag gtttctgctt ctaaagccgc tatagaggcg
     2761 gcacgtacca atatcattca ggcgcaaacc cgcgtcgaag cggcacaagc cactgaacgg
     2821 cgcattgccg cagatatcga tgacagcgaa ctgaaagccc cgcgtgacgg acgcgtgcag
     2881 tatcgggttg ccgagccagg ggaagtgctg gcggcaggcg gtcgggtgct gaatatggtc
     2941 gatctcagcg acgtctatat gaccttcttc ctgccaaccg aacaggcggg cacgctgaaa
     3001 ctgggtggtg aagcccggct gatcctcgat gccgcgccag atctgcgtat tccggcgacc
     3061 atcagttttg tcgccagtgt cgcccagttc acgccaaaaa ccgtcgaaac cagcgatgaa
     3121 cggctgaaac tgatgttccg cgtcaaagcg cgtatcccac cggaattact ccagcagcat
     3181 ctggaatatg tcaaaaccgg tttaccgggc gtagcgtggg tgcgggtgaa tgaagaactt
     3241 ccgtggcctg acgacctggt ggtgaggttg ccgcaatgac gcatctggaa ctggttcccg
     3301 tcccgcctgt cgcgcaactg gcgggcgtga gccagcatta tggaaaaacc gttgcgctga
     3361 acaatattac tctcgatatt ccggcccgct gtatggtcgg gctgattggt ccggatggcg
     3421 tcgggaagtc gagcttattg tcgttgattt ccggtgcccg cgtcattgag cagggcaatg
     3481 tgatggtgct gggcggcgat atgcgcgacc cgaggcatcg ccgcgacgtc tgcccgcgca
     3541 tcgcctggat gccgcagggg ctgggcaaaa acctctacca caccttgtcg gtgaatgaaa
     3601 acgtcgattt tttcgctcgc ctgttcggtc acgacaaagc ggagcgggaa gtgcgaatta
     3661 atgagctgct gaccagcacg gggttagcac cgtttcgcga tcgtccggca gggaaactct
     3721 ccggcgggat gaagcaaaaa cttgggctgt gctgcgcgtt aatccacgac ccggaactgt
     3781 tgatccttga tgagccaaca acgggggttg acccgctctc ccgcgcccag ttctgggatc
     3841 tgatcgacag tattcgccag cggcagagca atatgagcgt gctggtcgcc actgcctaca
     3901 tggaagaggc cgaacgcttc gactggttgg tggcgatgaa tgccggagaa gtgctggcaa
     3961 ctggaagcgc cgaagagttg cggcagcaaa cgcaaagcgc cacgctggaa gaggcgttta
     4021 taaatctgtt accgcaagcg caacgccagg cgcatcaggc ggtagtgatc ccaccgtata
     4081 aacctgaaaa tgcagagatt gccatcgaag cgcgcgatct gaccatgcgt tttggttcct
     4141 tcgttgccgt tgatcacgtc aatttccgca ttccacgcgg ggagattttt ggttttcttg
     4201 gatcgaacgg ctgcggtaaa tccaccacca tgaaaatgct caccggactg ctgcccgcca
     4261 gcgaaggtga ggcgtggctg tttgggcaac cggttgatcc aaaagatatc gatacccgcc
     4321 gtcgtgtggg ctatatgtcg caggcgtttt cgctctataa cgaactcacc gtgcggcaaa
     4381 acctggagtt acatgcccgt ttgtttcaca tcccggaagc ggaaattccc gcgcgagtgg
     4441 ctgaaatgag cgagcgtttt aagctcaacg acgttgaaga tgttctgccg gagtcattgc
     4501 cgctcggcat tcgccagcgg ctttcgctgg cggtggcggt gattcatcgc ccggagatgt
     4561 taatcctcga tgagcctact tctggtgtcg atccggtggc gagggatatg ttctggcagt
     4621 tgatggtcga tctctcgcgc caggacaaag tgactatctt catctccacc cactttatga
     4681 acgaagcgga acgttgcgac cgcatctcac tgatgcacgc cggaaaagtg cttgccagcg
     4741 gtacaccgca ggaactggtt gagaaacgcg gagccgccag tctggaagag gcatttatcg
     4801 cctatttgca ggaagcggca gggcagagca acgaagccga agcgccgccg gcgatacacg
     4861 acaccaccca cgcgccgcgt cagggattta gcctgcgccg tctgtttagc tacagccgcc
     4921 gcgaagcact ggaattgcga cgcgatccgg tccgttcgac gctggcgctg atgggaacag
     4981 tgatcctgat gctgataatg ggttacggca tcagtatgga tgtggaaaac ctgcgctttg
     5041 cggtgctcga ccgcgaccag accgtcagta gccaggcgtg gacgctcaat ctctccggtt
     5101 cccgttactt tatcgaacag cggccgctca ccagttatga cgagcttgat cgtcggatgc
     5161 gtgcgggcga tatcacggtg gcgattgaga tccctcccaa tttcgggcgt gatatcgcgc
     5221 gtggtacgcc tgtggaactc ggtgtctgga tcgacggagc gatgccgagc cgtgccgaaa
     5281 cggtaaaagg ttacgtgcag gccatgcacc agagctggtt acaggatgtg gcgagccgac
     5341 aatccacacc cgccagccaa agcgggctga tgaatattga gacgcgttat cgctataacc
     5401 cggacgtaaa aagcctgcca gcgattgttc cggcggtgat cccgcttctg ctgatgatga
     5461 tcccgtcaat gctaagcgcc cttagcgtgg tgcgggaaaa agagcttggg tcgattatca
     5521 acctttacgt gacccccacc acgcgtagtg aatttttgct tggtaaacag ttgccataca
     5581 tcgcgctggg gatgctgaac tttttcctgc tctgcggcct gtcggtgttt gtgtttggcg
     5641 taccgcataa aggcagtttc ctgacgctca ccctggcggc gctgctgtat atcatcattg
     5701 ccaccggaat ggggctgctg atctccacct ttatgaaaag ccagattgcc gccattttcg
     5761 gaacggcgat tatcacgttg atcccggcga cacagttttc cgggatgatc gatccggtag
     5821 cttcgctgga agggcctgga cgttggatcg gcgaggttta cccgaccagt cattttctga
     5881 ctatcgcccg cggaacgttc tcgaaagcgc tggatctgac tgatttgtgg caacttttta
     5941 tcccgttgct gatagccatc ccgctggtga tgggcttaag tatcctgctg ctgaaaaaac
     6001 aggagggatg atgcgccatt tacgcaatat ttttaatctg ggtatcaaag agttgcgcag
     6061 tctgctcggt gataaagcga tgctgacgct gattgtcttc tcgtttacgg tgtcggtgta
     6121 ttcgtcagcg accgttacgc ctggatcgtt gaacctcgcg ccgatcgcca ttgccgatat
     6181 ggatcaatcg cagttatcga accggatcgt taacagcttc tatcgcccgt ggtttttgcc
     6241 accggagatg atcaccgccg atgagatgga tgccggactg gacgccgggc gttatacctt
     6301 tgcgataaat attccgccta attttcagcg tgatgtcctc gccggacgcc agccagatat
     6361 tcaggtgaac gtcgatgcca cgcgcatgag ccaggcattt accggcaatg ggtatatcca
     6421 gaatattatc aacggtgaag tgaacagctt tgtcgcgcgc taccgtgata acagcgaacc
     6481 gttggtatcg ctggaaaccc ggatgcgctt taacccgaac cttgatcccg cgtggtttgg
     6541 cggggtgatg gcgatcatca acaacattac catgctggcg attgtattga ccggatcggc
     6601 gctgatccgc gagcgtgaac acggcacggt ggaacattta ctggtgatgc cgataacgcc
     6661 gtttgagatc atgatggcga agatctggtc gatggggctg gtggtgctgg tggtatcggg
     6721 attatcgctg gtgctgatgg tgaaaggtgt actgggcgta ccgattgaag gctcgatccc
     6781 gctgtttatg ctgggcgtgg cgctcagtct gtttgccacc acgtcaatcg gcatttttat
     6841 ggggacgata gcgcgttcaa tgccgcaact ggggctgctg gtgattctgg tgctgctacc
     6901 gttgcaaatg ctttctggtg gatccacgcc gcgcgaaagt atgccgcaga tggtgcagga
     6961 cattatgctg accatgccga cgacacactt tgttagcctc gcgcaggcca tcctctaccg
     7021 gggttccgga ttcgaaatcg tctggccgca gttcctgacg ctgatggcaa ttggcggcgc
     7081 gtttttcacc atcgcactgc tgcgattcag gaagacgatt gggacaatgg cgtaaggaag
     7141 ggaaacttga cttcatactg tgatgtcata ctattgatgt cataaagtga agtcataagg
     7201 acatgccatg agaaccaagt tacaggcttt acggaagaaa caaaaaaata cattggatca
     7261 aatatttaaa actcctgttc ctcaagggat caagtggtct gatatagagt cactggttaa
     7321 agcattaggc ggagaaatta aggaaggaag aggttcgcgt tgtaagttca tactaaatat
     7381 gagcgttgcg tgtttccatc ggcctcatcc gtcgccagat accgataaag gcgctgtaga
     7441 aagcgtgcgt gactggttac taagtatagg ggtaaaacca tgatcaaact gaagacgcca
     7501 aactctatgg agattgccgg gcaacctgca gttatcactt acgtgccaga actgaatgca
     7561 tttcgtggca aattcctggg cttgtcaggt tattgtgatt ttgtctctga cagcattcag
     7621 gggctgcaaa aagaagggga actttccctg cgagagtatc ttgaggattg taaagctgcg
     7681 ggtattgagc cgtatgcgcg tacagaaaag attaagacct ttacgttacg ctatccagaa
     7741 tcgctgagtg aacgccttaa taatgcggct gcgcagcaac aagtctctgt taatacctat
     7801 attattgaaa cactgaatga gcgtttgaat catttataaa ttttagcggc gtacattacg
     7861 cagaatatct gcctttacag gatctatcta tgcaactgaa cttaatttta taaattaagt
     7921 tcagttaata tcttttcttt cctcgtttca gaaataactc aatcttcctt cggcaggcac
     7981 tgcaaatgcc cgtgccgcac gccgcgctgg gcgataacgt catcggcaaa atgctgcacg
     8041 tcgcccatat cacctttcag cacggcgatt tccagacagt cgtcgtgatt gatatgcaca
     8101 tgcagcgtgg cgacggagag atcgtggtga tggtgctggg tggagacaat gcggctggct
     8161 aagtcgcgtt tttcgtgttc atacacatag gagagcaccg cgaaaccttg cgtgccgtgc
     8221 tgctgagtgg cctcctgggc cagggcgcta cgcagaatat cgcggatagc ttcggaacgg
     8281 ttgttataac cacgacgctg gctcaggctg tccagcgtct ccagtaaatc gtcatcaagc
     8341 gtgatggtga ctcgttgcat ttgcgttaaa ccttttctgt ggtgcgacgg cgcacgggga
     8401 atgcgggtaa taccgcgttt tgtagcacac gtccggcgtc agaggaaaag gttaatttat
     8461 ctcccaccac ctgggtttcg acgatttgtc cgttgtccat caccatcacc cgctgacaga
     8521 agcgttccac caggcgtaag tcgtgggtga tgaacaggca ggcggtgcca aactgttgtt
     8581 gtagcttttt cagcaggcga atgacgcccg cctgtaacac gagatcaagg ttagaaacgg
     8641 cttcatccag aatcagtagt ttcggttcga ccgccagcgc gcgagccagg cagacgcgct
     8701 ggagctggcc gccgcttaac tgcggtgggc gtttgtcgag aacgctgtca tcgagatcga
     8761 ccgccttcag catttcgctg gcgcgcgcca gttgttcgga ttttttcagt gatagcaggt
     8821 ggcgcatcgg ttcacgcagg atctcgcgca cggttttgcg cggattcacg gcgctgatgg
     8881 agtcctgaaa taccatctga atatcgcggc ggaacgcttt acgctgggcg cgattgagtt
     8941 tcgccagcgg ttcgccgcgc cagctaatat tcccttgtga gggagactct aagcctacca
     9001 gcagccgcgc gagggtgctt tttccgcagc cgctgcgtcc cagcagggcg acggtttcgc
     9061 cgcttttcag ggtcagggaa acgttattca gcaccgcctg atgttgatgt tttccgctaa
     9121 atccaccgtg ggcatagtga tgggaaaggc cgcagacgtt aagtaaggtc atgatgccag
     9181 ctccataccg tagagggcga gatgagcgga aaccaggctg cgcgtcaccg tatgtttggg
     9241 ggcgttaaac agcgtttcta catcgccctg ttcgacaatt ttaccttgtg acatcaccgc
     9301 cacgtcatcc gccagacgcg ccacaacgcc catatcatgg gtgaccagca gcattcccgg
     9361 cgcttgtttt tgcataatgc tttccagcag atcgaggatg cgcgcctgtg ctaccacgtc
     9421 gaggtcggtg gtcggttcat cggcgatgat aaacggtgat tcacacagca ccgccatcgc
     9481 aatcatcatg cgctgcaaca tgccgccgct catctcgaac gggtacagct tcagcacgcg
     9541 cgcggcgttt tccagcccca ccgcttctat ggcagcggta agcgtagcgt catcggcggg
     9601 tttccctaac gccaggtagg tttcccgcgc gtgggtgtgc atggtgtgca gtggattaaa
     9661 ggcgctgcgc gggttctgca tgatggtggc aattttgatg ccgcgcaggg cgcaaggcga
     9721 aaccggtttg ccatcggcta aaatttcccc cgccgtctgg cgaacgccag cgggcaaaat
     9781 gcccagcgtc gcggcgcagg ttaatgattt cccgctgccg ctaccgccga ctaacgccag
     9841 cacgcgcccg cgttgcaggg ttaacgatac accgtgtacc agcggctgcg cggcctgtag
     9901 cgcgatatta cgtagttcaa tctgttgcgg cattagtgtg cgtgctccgt caccagatga
     9961 gggtccagat gatcgcgcag tgcgtcaccc accaggttaa aggccatcac gctgataaac
    10021 agcgccagcc ccggccagaa catttgcagc ggctgggtcc agatatactg gcgcgcgtcg
    10081 ttaatcatca cgccccattc ggcggtcggc gcggtcacac cgaggccaag gaaagacatc
    10141 cccgcgacgt gcagcatcat atggccgata tccagcgttg ccagcaccag cagcgaaggg
    10201 atcaccgcgc ctgccagatg atcgacaaac acccgcacat ggcccgcgcc agaaagccgt
    10261 gacgccagca caaactcgcg ttggcgtagt gaaatcacca ggctgcgcac catgcgtgca
    10321 taccacgccc agtgcgacag ggcgatggcg ataattacgt tggtcagccc ggtgccgagc
    10381 acgccaacca taaagaacga cagaatcgag gtcgggaagg tcataaacat atcggcgacg
    10441 cgcatggtgg cctgatcaac gcgcccgcca atcaacccgg cgctgccgcc aataaccagc
    10501 cctaatgtca gcaccagcag caggcaggcc attaccgaac cgagcgacac gcgggtcgcc
    10561 gccatcagcc gcgagaaaat atcacgccct aagtgatcgg tgcccagcca gtgctgcgca
    10621 tccggcgaaa gcaggcgcga cggcagatca atagcctgag ggtcatacgg cagccaccac
    10681 tggctggtaa gcgcaatcag cgccagcagg gcgataatga tcagcgccag gcgtaccgac
    10741 cagcgggaag agaggaaaaa gttcacgcgt gcgctccttc atgacgacga atgcgcgggt
    10801 ctagcgcggc gttgagcaaa tcgacaatca aattacagac cacaaaaacc accaccatca
    10861 tcagcgtaaa gcactggatc accggatagt cacggttaaa aatcgccgac accgcatagc
    10921 gcccgacgcc cggccaggca aagatgtttt cgataatcat cgtcccgcca atcagttcgc
    10981 cgatgtgcat ccccacggcg gtgatcatcg gcagcgaggc attgcgcagg atgtggcgac
    11041 gttcggtctg tttgtcgttc aggccgcgca gacgcgccca ggtgacgtga cgctgaccgg
    11101 cgacgtccag catactggcg cgcagtaaac gcgcgttaat cgccagcgac ataaaggcaa
    11161 tggaaaccgc aggcaaaatg atgtgctgcc agccgccgta acccatcgcg ggtagccatt
    11221 gcagatacac cgaaaacgcc atcaccagta aaaacgccag ccagaagtta ggcatcgaca
    11281 cgccaagaaa tgcgatgaaa cgtacggcga aatccggcaa acgatcgcgg tggcgcgccg
    11341 cccagatacc gagcggtacg gaagtgagca gaattaatac cagcgccgca cctgcaagtt
    11401 ccagcgtggc gggcaggaag ttcagcatat cgtccagtac cgggcgttgg ctggcgaatg
    11461 agataccaaa gtcaagatgc agcgccttcc acaaccaggt accgtactgg acgtacagcg
    11521 gctgatccag cccgagcatg gtgcgggtag aggccagcat ctccggcgtc ggcggcaggt
    11581 tagataaacg cagataatcg agcgccgggt cgccggtacc gaggcgcagc atcagaaaaa
    11641 tgatcaccga ggcggcgagc accatcggga tcagcagcag aaagcggcgt aatacgtaac
    11701 gcaacattaa ggtttcaccg gtttaatctg ttcgaacgga atttcggtgg cgatcggcgc
    11761 gtaggggata ttacccagct ccggttttga taccaccatc atcgagatgt agctgatagg
    11821 cagataaacc gcctcgtcat gcagacgggt cagaatgtcg cgatacagcg cctgacgttg
    11881 cgtttcgtca tgggtcgcca gcacttcgcc gatctcttta tcaatcagcg gcttatcggc
    11941 taatccttgc tgtgcctgga agtcagcgtg cgacggtacg cgcattgaac tcaggaaggc
    12001 gtgtggatcg tatggcgcgc cccaggtgcg gtggaaaatc atgccaaaac gaccgtcgcg
    12061 ctgacgagca tagatactgc tctcttcttc gccaatcagc gagacatctg cgccaatctg
    12121 gcgcatatca gcctgaatga tttccgccat cgatttgctt aacgcatcgg tgccgatgaa
    12181 cgacagttca atgcgcagcg gctgaccatt tttctcgcgg atgtctttgc ccgcaggcag
    12241 cgtccaaccg gctttttcca gcaacgcttt cgctttctgc ggatcgtact ggcgcggttt
    12301 caggccgaga ttagcgtaag gcacagaagg ggcaaacagg gtgtcggcga cctgctgggt
    12361 gccatataac gcgttatcaa tcagcgattt tttgtttacc gcgtaattaa gagcttcacg
    12421 tactgccagc tcgttggtgg gggctttggc ggtattgagc gccagcatca cggtttcgat
    12481 cggctgtgac agttgggtgt ggtaagccgg attctggcta aagcgggcga aggtatcgag
    12541 cggtaataac ccttcgtttc cgtacagcag gtcgatatcg ccagtttcaa acgccaccgc
    12601 gcgggtagtc gggtccggga tgacgttaaa ggtgatcttt ttaatcgctg gcttttcgcc
    12661 ccagtagttt tcgttacgga cgaagacatc gtactgattc agtttcgatt cctgcaaaat
    12721 ccatggtccg gtgccaatcg gcgctttaat tccgttcatg gtttcatggt ttttaaactg
    12781 cgagggagcg ataaaacgga aaggacgggg cagggccagt tcttgcagga aaggatagta
    12841 ggcgcttttc agggtaattt gcagctctgt tttactgagt gctttaacat caacaatctg
    12901 gtttgccagc tccagccagg cgtgacgttg acggttatcg agcactgcgc ggaagttttc
    12961 tgccgccgcc tcggcatcga acggttcacc gttggagaat ttcacgtcat cacgcagggt
    13021 gaaggtccag gttttaccat cttctgaatg agtccagctt tttgccagcc acgggatcac
    13081 cgaaccgtct gcctgatatt tcaccaatgg ttcataaacc atgctctggg cgaacatctg
    13141 gttaggcgtg taaaggtgcg ggtttagtgg cccgacattc accggccagg cggtggtgat
    13201 ttcatctggt gcagcggcat ggacgataaa agacgcacaa gccagcagcg caaatagagt
    13261 gcggcggagt gtggagagca tggtaacccc aatggattaa aatagatggc ggaaataagt
    13321 atgacgattt taagtattcg tcatactgat tacctgttct ggatcaatag atgggcagtc
    13381 gggtgggcgg atcagttaac tgaatcgatc cattgcacac tgtcggcggt gagcgtaaag
    13441 ggggtggggg tgcagatcgc aaggcttaaa ttttcaagct gacaatggct gactgacagc
    13501 gaggagtgat aggtgctatc tacgctgacg atttgccagg cgctgccgcc acgctgttta
    13561 acgatggctt ctttgcgcgt ccagatgcgc cagaacattt ccagttgctg atcaggatgc
    13621 acggcgtcca tttcagcgtg ttccccgagg ctgaacacgg cgttcgccag ccagcgccag
    13681 ttggcgcgcg ggcgaatcac ttcgatatcg cagccgactt cgccttcatc gctcaacagc
    13741 agagcgatat cgtcaccgct atggcttaag ttgaaccata gcggcgtttc cggcgcgaat
    13801 gcaggtttgc cttgttcgcc atagatgatc tccggtagcg gggaaagcgt gtgcgaaagc
    13861 aatgcacgcc ccgccagcca gcgttcgcgt cgtggacctt gcggtgcttg ctcgcgtaaa
    13921 cccggtggca gtggagctgc gcttaaggtc gaaactttcc ccagaactat ccgatacata
    13981 tcagggccaa cgtttaatgg aaaatgaaag tgcgtatcgt atcacttgtc gcctcatccc
    14041 ggtaaccgac ttttcggtct gcctggcccc agtaaaatcg ccagtttgct accgcctttg
    14101 gtggtttcca tccagatttt acacacgctg gttaatggca ccgaaagtag catccctacc
    14161 gggccgagca gccatcccca aattaacaat gaaagaaata ccaccatggt ggacatcccc
    14221 aggcgatggc ccatcatccg tggttctaaa atattgccga tgaccatatg gacaactaaa
    14281 aacaatgcgc cgaccagaat acattcgtaa ataccattaa acagcagcac ctgaatcatt
    14341 ggcggtacgg cggaaattac cgcgccgata ttgggcacgt agttaagcaa aaacgccagt
    14401 actgcccaca tcagcgcaaa ctgcaccccc atcagctcca gccccagcca gacgatgaca
    14461 cccgtccata aactgagtag cgtctttaac gccagataat gcgaaacgcc tttaagtgcg
    14521 cggtgtaatc ccgcgatgtg aatctgtggt ttattcagcg caaaacgcat tttgtaaggg
    14581 acgtggcgca cttcaaacag cataaaaact acggtcatca ccagcagaat tacgctcgcc
    14641 attgccccgg aaagcccggt cattagcgcc gtggtgaagg taaccacttt ttccgagtcc
    14701 atccgctgca gcattcgctc cggcgacata tgcaaattaa gaaaaggcaa catctcctgc
    14761 aatttaaaaa gtttgcgcgt cagctcctta ttaaacttcg gcagcataga gataaattcg
    14821 ttaaacgatg ccgccagtac gccgaccagc gcggttagtg cgatcagcat caccaccacc
    14881 acaatcgtaa tggcaacggg gcgttttact ccccgtcgaa caaaccaggt aacgagcggg
    14941 ttgaggacga tggcaaaaaa cagcgccagt aacagctgca caatgatatc tgccgctgcg
    15001 tgaatgcccg cgaggatcac taccagcgag gccagcttga gcagaatgtg catgcccgtt
    15061 ttatcgggtt gaggggtttc cattgggctt ccttgtgact ttttgtatta agtgtagcgg
    15121 gagtcacgcc agcaatattc tgattcttac tgctgatttt cacgtcagca cgtggtaaaa
    15181 atgaaacact gttgtaaaaa tgtggtgatc ctcatgcccg aacccgtagc cgaacccgcg
    15241 ctaaacggat tgcgcctgaa tttgcgcatt gtctccattg tcatgtttaa cttcgccagc
    15301 tacctcacca tcgggttgcc gctcgctgta ttaccgggct atgtccatga tgtgatgggc
    15361 tttagcgctt tctgggcagg attggttatc agcctgcaat atttcgccac cttgctgagc
    15421 cgccctcatg ccggacgtta tgccgatttg ctgggaccca aaaagattgt cgtcttcggt
    15481 ttatgcggct gctttttgag cggtctgggg tatctgacgg caggattaac cgccagtctg
    15541 cccgtcatca gcctgttatt actttgcctg ggacgcgtga tccttgggat tgggcaaagt
    15601 tttgccggaa cgggatcgac cctgtggggc gttggcgtgg ttggctcgct gcatatcggg
    15661 cgggtgattt cgtggaacgg cattgtcact tacggggcga tggcgatggg tgcgccgtta
    15721 ggcgtcgtgt tttatcactg gggcggcttg caggcgttag cgttaatcat tatgggcgtg
    15781 gcgctggtgg ccattttgtt agcgatcccg cgtcagacgg taaaagccag taaaggcaaa
    15841 ccgctgccgt ttcgcgcggt gcttgggcgc gtctggctgt acggtatggc gctggcactg
    15901 gcttccgccg gatttggcgt catcgccacc tttatcacgc tgttttatga cgctaaaggt
    15961 tgggacggtg cggctttcgc gctgacgctg tttagctgtg cgtttgtcgg tacgcgtttg
    16021 ttattcccta acggcattaa ccgtatcggc ggcttaaacg tagcgatgat ttgctttagc
    16081 gttgagataa tcggcctgct actggttggc gtggcgacta tgccgtggat ggcgaaaatc
    16141 ggcgtcttac tggcgggggc agggttttcg ctggtgttcc cggcattggg tgtagtggcg
    16201 gtaaaagcgg ttccgcagca aaatcagggg gcggcgctgg caacttacac cgtatttatg
    16261 gatttatcgc ttggcgtgac tggaccactg gctgggctgg tgatgagctg ggcgggcgta
    16321 ccggtgattt atctggcggc ggcgggactg gtcgcaatag cgttattact gacgtggcga
    16381 ttaaaaaaac ggcctccgga acacgtccct gaggccgcct catcatctta aaacttactg
    16441 aataaccagc gtattaatga tgttttctgc ggtggtctgc gctttttgct gatcgtcagc
    16501 gggcagcgta atttgcatgg tcagcagttg attacccacg ttacccagaa taacggaaga
    16561 gtacgccgtc tggcctttcg cggagataat actgtctaac tgctgcattt tgtgaccttt
    16621 cagctcaatg gctttattgg ttaccacttg cagctgcgga tcgcggctac gttgctgatc
    16681 ttccagacgc ttcgccagca ccgccagatc ttctttcgga tcatcgccca tgatgacgat
    16741 gactgctttc tgcccggtgg cgtcggacca gacatgcatg ttattggcct gcgttcccag
    16801 cttaccgctc tggtcggtca tatccgctgg cagcgagaaa cttaacttgc catcaagcag
    16861 gttgacggga ttcccggtag cgttactttc cgcgaccgaa ccctgcgccg tagcgttagt
    16921 gtctttatca tcacaggccg caagccccat aaccagcagg ccaattccga catatttaac
    16981 cagattgcgc attgacttct tcctttcgat aaacggccat aacggctcat tcatccatct
    17041 tatcacaact ctgataacga acctttaact cgcctgcaaa gcgttgattt cggatttatc
    17101 tgccagtctt ttcaacagca tattgagtaa tacgccatac attggcagga agaaaacgat
    17161 actgattaac actttgaaac agtaatcgac cagcgcgatt tccatccagt gttcagccat
    17221 aaaggcatcc gggctacgcc agaaggcaat gaagaaaaag gccagcgtgt cgctgacgtt
    17281 accgaacagt gtggacgctg tcggtgccag ccaccagcgg cgactctgac gcaggcggtt
    17341 aaaaacgtgc acgtcgagga tctgtcccag cgcgtaggcc atgaaactgg cggtggcgat
    17401 acgggcgaca aacaggttga agtgggcgag tgcgccgaat ccctgccagg aacccatata
    17461 gaatagcgac gagatgacgt aggagatcag caacgccggg atcatcaccg caaagatgat
    17521 acgacgggcc agcggtgcgc caaaaatacg cacggtcagg tcggtagcaa ggaaaataaa
    17581 cgggaagcta aacgcgcccc aggtggtatg gaaacccaaa atggagacgg gaagctgaac
    17641 cagatagtta ctggaggtga tcaccagcag atgaaataac gataaccaga acaacgcctt
    17701 atagcgttga gtttgcgaga aaacgttcat attgtacctt tttgattaac cattggggtg
    17761 agggaaccca atacgtacga cacgtctgtt accttaacat aagaacgtgc ttaccctttt
    17821 ttcgagccgc cgcatgatac tgctttacgt tgacaatgca atggttaatt ttcacgcaat
    17881 cgttaacctg gtttgcttac ggactcacag ggcgtaaact agcgccgttt ttttatgtga
    17941 tgagaagaaa atgaccgctc tcttttccag ccctgaccac acactcgacg cacttggcct
    18001 gcgctgcccg gaacccgtaa tgatggtgcg caaaactgtg cgcaatatgc agcctggcga
    18061 aacgttgctg attatcgccg acgatccggc gacgacccgc gatattcctg ggttttgtac
    18121 ctttatggaa cacgaactgg ttgcgaaaga gacggatgga ctgccttatc gttatttgat
    18181 tcgtaaaggc ggttgatggg ggctgattgg cttcgatgcc gccttttccc ctcaccctaa
    18241 ccctctcccc agaggagcga ggggaccgat cgcgctcaat gttgcgatcg gtttgcctta
    18301 tctcctgcgc aacaatctta acgcattcgc tgtcaccagc accgtcgcac ccgtatctgc
    18361 cagcactgcc agccatagtc cggtcatccc taacagcgtg gtgacgagga agatcccttt
    18421 cagccccagc gcaatggtga tgttctggcg gatattggcg tgagtggcgc gtgccagttc
    18481 aatcatttgc accaggccgc gcaggtggtt atgggttaat gctgcgtcgg cggtttccag
    18541 cgccacgtct gtgccgctac ccatagcaat cccgatggcg gcagctttca tcgctggcgc
    18601 gtcgttaata ccatcaccga ccatcgccag gggcgcatgt tgattcagct cggtcacggc
    18661 tttgacttta tcttccggca ataggcccgc tttaaactcc agccccagct ccccggcaat
    18721 tgccgccgct gcgcgtggat tatcgccggt gaggatcact cctttaaccc ccagcgcgtt
    18781 cagttcactg atggcagttg cagcgtcggc gcgcagggta tcctgcaatg caatgatacc
    18841 cagcacgtca tcattacgta ctaccagcac aaccgtttgc ccggcgcttt ccagttcatt
    18901 aatcaaacca gcaaatgcat cagcgggatg tttcccggca gcgcatatca acacgcgctc
    18961 accgttaacc tgcgcttcaa tgccagaccc gaccagtgcc cgctgtgatt cggcggtggg
    19021 aatggcgagt tcagcaacct gtgcttctcg cacgatggct tgcgccagtg gatgcgtcgc
    19081 gccttgctcg accgctgccg ccagtgtcag cagttcagat tcactaatac ccgttgccgg
    19141 atgaatcgcg gtaacgcgcg gtttaccgac ggtcagcgta ccggttttat caaacgccac
    19201 ctgagtaaca cgacctagtt gttccagcgc cgctccgcct ttaatcaacg ccccacgacg
    19261 cgctgctgcc gccagcccgg aggtaatcgc cgcgggcgtg gagataacta acgcacacgg
    19321 gcagccaatc agcagcagcg tcaggccttt ataaatccac tcctgccagc tggcggcaaa
    19381 cagcagcggt ggcaccagcg tcaccagcag agcgacggcc ataatcgcgg gcgtatagat
    19441 acggctgaaa cggtcgataa accgctcaat gggggcgcga cgctcttcgg cttcttcgat
    19501 cagtttcaga atccggtcaa tggcgctggc acccggttct gacagcactt ccagcgttac
    19561 cagacggtct acgctggtgg ctcctgcagg aactttatcg cccgtcgcgc gctccaccgg
    19621 aatggattct ccggtcaggg cgctttcatc aaaactggca aacggtgaga gcagtttacc
    19681 gtcggcaggc aaacgcccac ctgcggcgac ttcaatcaca tcgccaggcc gcaggctgtt
    19741 aatcgccacc tcttcccgct caccgttacg caggcgcgtg gcggtttctg gtttcagcgc
    19801 cattaacgcg ctaacgccct gacgcgcgcg gctggcggcc cagccttcca gtcgttcacc
    19861 aatcaaaaac agcagcaaca ccatcgcagc ttcagccgtt gcgccaataa acagtgcacc
    19921 aatagcggct acgctcatta aggtttcaat ggcgaagtag ctgccggatt tgatcagccg
    19981 tgatgcctga cgagcaatcg ggtacagccc aaccagcgtg gtcgcgataa acgccagttg
    20041 cccgaacgga tgattgaact gctccagacc ccagctgatt gccatcatca cgattagtgt
    20101 aatcagcggc agattctctt tcaggcgcga tgcttgtggc tcgtcggcgg cctgctcatc
    20161 gcgcagggaa tagcccgctt tttgcaccgc agattcaact tgtgcacgaa tgtcattgtc
    20221 ggcatcaacc accagttttt cggtggcgaa caacacctgc acctgattca cacctgcaag
    20281 ctggcgcacg gcattttcta ccttacgcgc acaggcggcg cagtccatgc cactgacttt
    20341 ccagctatag cgggtgccgg agacgttttc agagagcgtt ggcgagctgg agcatgcgcc
    20401 gtcgcagcaa cagtcgttgg cgttctgtac cgtggttagc ggtttgaacg cagcaaattg
    20461 aggggctttc ttgccgtgat tgtcaggtgt cgacatggca tactccggtt aagttttttc
    20521 tcattaaccg aaggatacac tctggagtcg actccagagt caagttttat cagagataca
    20581 gtgagcggac gatgaggaag tgaccggcaa agtagcaggc cgcagcaatc gcgttatccg
    20641 cacggaagcg tcggcgatag tggctcccca gccagacaaa gttactgata aacagcaacg
    20701 aagcgccaac aaacgcagag agcgccggag cggtcggacg gaagaaccac agttcacctg
    20761 ccagccacac catcaccagc gtcatgccga taaacgtgca gataggccaa cgatactctt
    20821 ccagccgcgt ccagataatc gccagtaaca gcgcacccag aaccagcaac accagtggca
    20881 gaggccagaa gaaagagagc gtcatctgac tggcgaaata gatggtgtac agcaggtgcg
    20941 agaggaaaaa cgcgccgatg gcgtacatca gacgttgacg tggcaacagg gttagcgcat
    21001 cgcccagcag tgaggcgcac agccctgcca gcaccagata gctaatggcg tcgaacatcg
    21061 gcgcttgcca ggccagtaac agcaggagaa gaagggttaa cggtttaaac acccagcgtt
    21121 gccaggttgg cccacgatac gatgcatcca cagatagcca tgcggaaaga cagacagcga
    21181 taaacgacca aagcatctta gttccttgaa ttcgttattt tttcgtaagc ggaatgccta
    21241 cgctcttgct tccagtttag tggcgagggt ggacggatga caacaccata actggcaggg
    21301 tatgcttatt tccgcatttt ccgatgaggg ataaagatga gcaaaccacc tcttttcttt
    21361 attgttatca ttggcttaat tgtcgtcgcc gcatcgtttc gttttatgca acagcgacgg
    21421 gaaaaagctg ataatgatat ggctccgctc cagcaaaagc tggtggtggt gagcaacaag
    21481 cgggaaaaac cgattaacga tcgccgttcg cgccagcagg aagtgactcc ggcaggcacc
    21541 agtatgcgct atgaggcaag cttcaaaccg caaagcggag gaatggagca gacgtttcgc
    21601 ctcgaggccc agcagtacca cgccctgaca gtgggcgata aaggtacgct gagctataaa
    21661 ggaacgcgct ttgtcagctt tgtaggcgaa caataaggtt attttttaac tttaaatttc
    21721 ttctgccaga ccagcagttc aaacacgccg aaaaggaaaa tccgcacctt ttcggcggtg
    21781 gtcatctgcg ggccatcttt cggtaaagtg gatttcaata gcgccagttg cataccatgc
    21841 atcagcacgg taaaaatcag cgccacgtta acgaaaatat tcagcgggcg cgggaaggga
    21901 tgcaccagat tgaggattaa aaatccccaa acgcagagca ttaacaaacg accaatatta
    21961 atcagcatca ctttctcctt gtgcttcgcg ttgatacagc cgataagcca cctgacccgc
    22021 cactttttcc cgatgtaatg accagtttgc cggaacagtg ggcagaccgt tttcgacttc
    22081 gctttcgaca taaatcaggg cttcgtcagc cagccagccg ttatcttcca gtaaatttat
    22141 cgtctcttct aacaagccac ggcggaacgg tggatcgaca aacacgatat tatgcggtgt
    22201 acctttttgc gccaggaatg acatcgcgtt gctgttcacc acgcgtgcat tgcctgcttt
    22261 tagtgtcgcc agattcttaa ttaactgctg agaaaccgcg cgatccatct caatcaacgt
    22321 ggcccccgca gcgtagcgcg ataacgcttc cagtcccagc gcgccgctcc cggcgaagca
    22381 atccagacat tgggcgtcaa caatgaccgg agccagccag ttaaacaacg tttcgcgtac
    22441 gcggtcggtg gtggggcgca gacctgggct atcaggaacc gggagtttac ggcctcgcca
    22501 ctgcccgcca ataatgcgga tttggccgct gccggaatga ttcggttttt tcatgataaa
    22561 ttgctcaatc cgccagataa cacatacttg caggcggtga tgtgaattaa gttaagtgat
    22621 agactatttc atcatttttt tagctgctat gtacatagcg ttaacgctgt gccatgaagc
    22681 aacagcgagg agtgtagtcg caaatggcga aagaaaaaaa acgtggcttt ttttcctggc
    22741 tgggctttgg tcaaaaagag cagaccccgg aaaaagagac agaagttcag aatgaacaac
    22801 cggttgtaga agaaatcgtt caggcgcaag agcctgcgaa ggcttctgaa caagccgtcg
    22861 aagagcaacc gcaggcgcat actgaagccg aggcggaaac ttttgctgcc gacgttgtgg
    22921 aagtcactga acaggttgct gaaagtgaaa aagcgcagcc tgaagcggaa gtcgttgcac
    22981 agccggaacc ggtcgtagaa gaaacgccgg agccagtggc tatcgaacgt gaagagctgc
    23041 cgttgccgga agacgtcaac gccgaagagg tttcgccaga agagtggcag gctgaagcgg
    23101 aaaccgtaga gattgtcgaa gcggcggaag aagaagcggc taaagaagaa atcaccgacg
    23161 aagagctgga agcacaggcg ctggctgccg aagcggcaga agaggcggtg atggtggttc
    23221 ctccggtaga agagcagccg gtggaagaaa tcgctcagga gcaggaaaaa ccgaccaaag
    23281 aaggtttctt cgcgcgcctg aaacgcagcc tgttaaaaac caaagaaaat ctcggttccg
    23341 gatttatcag cctgttccgc ggtaaaaaaa tcgacgatga tctgtttgag gagctggaag
    23401 aacagctgtt gatcgccgat gtgggcgtgg aaaccacacg taaaattatc accaatctga
    23461 cggaaggcgc atcccgcaag cagcttcgtg atgccgaggc gctctatggc ctgctgaaag
    23521 aagagatggg cgagattctg gcgaaagtcg atgagccgct gaatgttgaa ggtaaaacgc
    23581 cgttcgtgat cctgatggtg ggcgtcaacg gtgtgggtaa aaccacgacg attggtaagc
    23641 tggcacgtca gtttgagcag cagggtaaat cggtgatgct ggcggcgggc gatactttcc
    23701 gtgcagcagc ggttgaacag cttcaggtct ggggtcagcg caacaatatt ccggtgattg
    23761 cccagcatac cggggcggat tccgcctctg ttatcttcga cgccattcag gcagctaaag
    23821 cgcgtaatat cgacgtcctg attgccgata ccgccggacg cctgcagaac aaatcgcacc
    23881 tgatggaaga gttgaagaaa atcgtccgcg tgatgaagaa actcgacgtt gaagcgccgc
    23941 atgaagttat gctgactatt gatgccagca ccgggcagaa cgcggtaagc caggccaaac
    24001 tgttccacga agctgttggc ttaaccggca tcacgctaac gaaactggac ggcacggcga
    24061 aaggcggggt aattttctcg gtggctgacc agtttggtat ccctatccgc tacattggtg
    24121 tcggcgaacg tattgaggat ttgcgtccgt ttaaggcgga cgactttata gaggcacttt
    24181 ttgcccgaga ggattaacaa tgattcgctt tgaacatgtc agcaaggctt atctcggtgg
    24241 gagacaggcg ctgcagggcg ttacgttcca tatgcagccg ggtgagatgg cgtttctgac
    24301 cggtcattcc ggcgcaggga aaagtaccct cctgaagctg atctgtggga ttgagcggcc
    24361 cagcgccggg aaaatctggt ttagcggcca tgacatcacg cgtctgaaaa accgtgaagt
    24421 tccgtttctg cgccgccaga ttggcatgat tttccaggat caccatctac tgatggaccg
    24481 tactgtctac gataacgtgg cgatcccgct gattatcgct ggtgccagcg gtgacgatat
    24541 tcgtcgccgg gtgtcggcgg cactggataa agtcgggcta ctggacaaag cgaagaactt
    24601 ccctattcag ctttccggcg gtgaacaaca gcgtgttggc attgcccgcg cggtggtgaa
    24661 taagcccgcg gtactgctgg cggacgaacc tactggtaac ctggacgacg cgttgtcgga
    24721 agggattcta cgtctgtttg aagagtttaa ccgcgttggg gtaaccgtat tgatggcaac
    24781 gcacgacatc aacctgattt cacggcgttc ctatcgcatg cttaccctga gcgatggtca
    24841 cttgcatgga ggcgtgggcc atgaataagc gcgatgcaat caatcatatt cggcagtttg
    24901 gcgggcgtct ggatcgcttc cgtaaatcgg tcggcggctc tggcgacggc ggtcgtaacg
    24961 cgccgaaacg cgcgaaatcc tcgccaaaac cggtaaatcg caaaaccaac gttttcaacg
    25021 aacaggtgcg ctatgccttc cacggcgcat tgcaggatct gaaaagcaaa ccgttcgcca
    25081 catttttaac ggtgatggtt atcgccatct ccctgacgct gcccagcgtc tgttatatgg
    25141 tgtacaaaaa cgttaaccag gcggcgacgc aatattatcc gtcaccgcaa atcactgttt
    25201 acttgcaaaa aacgctggac gatgacgccg ctgcgggcgt ggtggcacag ttgcaggccg
    25261 agcaaggcgt agagaaagtg aactatcttt ctcgtgaaga cgcgctgggc gagttccgta
    25321 actggtctgg ctttggcggc gcgctggata tgctggaaga aaacccgctt ccggcagtgg
    25381 cagtggtgat cccaaaactc gattttcagg ggacggagtc actgaatacg ctgcgtgatc
    25441 gtatcacgca gattaacggc attgacgaag tgcggatgga tgacagctgg tttgcccgtc
    25501 tggcggcgtt gaccgggctg gtcgggcgcg tttcggcgat gatcggcgtg ttgatggtgg
    25561 cggcagtgtt cctcgtcatc ggtaacagtg tgcgtttaag catctttgct cgccgtgact
    25621 ccattaatgt gcagaaactg attggtgcga cggatggttt catcctgcgc ccgttcctgt
    25681 atggtggcgc actgctggga ttttctggtg cattgttgtc attaattttg tcagaaattc
    25741 tggtgctgcg attgtcatcg gcggttgcgg aagtggcaca ggttttcgga acgaagtttg
    25801 atatcaatgg cttatcattc gatgaatgcc tgctattgct gctggtatgc tcgatgattg
    25861 gctgggtggc agcgtggctt gccacggtac aacatttacg ccactttacg cctgaataat
    25921 aaaagcgtgt tatactcttt ccctgcaatg ggttccgtag cagggaaaga gaccccgttg
    25981 tctcttcccg gtatttcatc tctatgtcac attttgtgcg taatttattc acaagcttgc
    26041 attgaacttg tggataaaat cacggtctga taaaacagtg aatgataacc tcgttgctct
    26101 taagctctgg cacagttgtt gctaccactg aagcgccaga agatatcgat tgagaggatt
    26161 tgaatgactg acaaaatgca aagtttagct ttagccccag ttggcaacct ggattcctac
    26221 atccgggcag ctaacgcgtg gccgatgttg tcggctgacg aggagcgggc gctggctgaa
    26281 aagctgcatt accatggcga tctggaagca gctaaaacgc tgatcctgtc tcacctgcgg
    26341 tttgttgttc atattgctcg taattatgcg ggctatggcc tgccacaggc ggatttgatt
    26401 caggaaggta acatcggcct gatgaaagca gtgcgccgtt ttaacccgga agtgggtgtg
    26461 cgcctggtct ccttcgccgt ccactggatc aaagcagaga tccacgaata cgttctgcgt
    26521 aactggcgta tcgtcaaagt tgcgaccacc aaagcgcagc gcaaactgtt cttcaacctg
    26581 cgtaaaacca agcagcgtct gggctggttt aaccaggatg aagtcgaaat ggtggcccgt
    26641 gaactgggcg taaccagcaa agacgttcgt gagatggaat cacgtatggc ggcacaggac
    26701 atgacctttg acctgtcttc cgacgacgat tccgacagcc agccgatggc accggtgctc
    26761 tatctgcagg ataaatcatc taactttgcc gacggcatcg aagatgataa ctgggaagag
    26821 caggcggcaa accgtctgac cgacgcgatg caaggtctgg acgaacgcag ccaggacatc
    26881 atccgcgcgc gctggctgga cgaagacaac aagtccacgt tgcaggaact ggctgaccgt
    26941 tacggtgttt ccgctgaacg tgtacgccag ctggaaaaga acgcgatgaa aaaactgcgc
    27001 gctgctatag aagcgtaatt tccgctatta agcagagaac cctggatgcg agtccggggt
    27061 ttttgttttt tgggcctcta caataatcaa ttccccctcc ggcaaaacgt caatccccac
    27121 gcagattgtt aataaactgt caaaatagct attccaatat cataaaaatg gggtatgttt
    27181 tagcagagta tgctgctaaa gcacgggtag tcatgcataa aacgaaataa agtgctgaat
    27241 cacaacacca caacacacgt aataaccaga agaatgggga ttctcaggat gaatataaag
    27301 ggtaaagcgt tactggcagg atgtatcgcg ctggcattca gcaatatggc tctggcagaa
    27361 gatattaaag tcgcggtcgt gggcgcaatg tccggtccgg ttgcgcagta cggtgaccag
    27421 gagtttaccg gcgcagagca ggcggttgcg gatatcaacg ctaaaggcgg cattaaaggc
    27481 aacaaactgc aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc ggttgcggtg
    27541 gcgaacaaag tcgttaacga cggcattaaa tatgtgattg gtcacctctg ttcttcatca
    27601 acgcagcctg cgtctgatat ctacgaagac gaaggcattt taatgatcac cccagcggca
    27661 accgcgccgg agctgaccgc ccgtggctat cagctgatcc tgcgcaccac cggcctggac
    27721 tccgaccagg ggccgacggc ggcgaaatat attcttgaga aagtgaaacc gcagcgtatt
    27781 gctatcgttc acgacaaaca gcaatacggc gaaggtctgg cgcgagcggt gcaggacggc
    27841 ctgaagaaag gcaatgctaa cgtggtgttc tttgatggta tcaccgccgg ggaaaaagat
    27901 ttctcaacgc tggtggcgcg tctgaaaaaa gagaatatcg acttcgttta ctacggcggt
    27961 tatcacccgg aaatggggca aatcctgcgt caggcacgcg cggcagggct gaaaactcag
    28021 tttatggggc cggaaggtgt ggctaacgtt tcgctgtcta acattgcggg cgaatcagcg
    28081 gaagggctgc tggtgaccaa gccgaagaac tacgatcagg ttccggcgaa caaacccatt
    28141 gttgacgcca tcaaagcgaa aaaacaggac ccaagcggcg cattcgtttg gaccacctac
    28201 gccgcgctgc aatctttgca ggcgggcctg aatcagtctg acgatccggc tgagatcgcc
    28261 aaatacctga aagcgaactc cgtggatacc gtgatgggtc cgctgacgtg ggatgagaaa
    28321 ggtgatctga aaggctttga gttcggcgta tttgactggc acgccaacgg cacggcgacc
    28381 gatgcgaagt aatcattaat cggcaacttt gggttgccgc caaattgcta atatcgagta
    28441 cgttgcttca tgccggatgc ggcgtaaacg ccttatccgg cctacaagat ccaaagaaat
    28501 cagtaaattg caacacacat tgtaggcctg ataagcgtag cgcatcaggc aatttctcgt
    28561 tggtcatcac ttttgaaatc aacttgacga ttaacgcttc tcccagccgc cctgttgcgc
    28621 cgtaaacccc agcgcctgca taaacgccgt catcacaccg cgatcttcca agcctgcatc
    28681 cgccatccac cagcatgaaa cggcaggatt gttacgcaaa acctcttcca gcagatattg
    28741 ccccacaccg cgacgtcggg tgacttcccg cacgcgcagg gaatccagtg ccccctcggt
    28801 gccgcttaat gttacccgca cggcagcgag caggcgctcg ttaaaacgcg cggcgtagat
    28861 acggtggtta tcgtcaacct gtaaagagga aggggaatac tccggccaga tcttttgcag
    28921 gtcaatccgg tcctggtcgc taaagttttc taatcgaatg atggtcagct tcatgggtaa
    28981 cccgtgtaaa tcaca
//
LOCUS       DAZKHC020000056        28589 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZKHC020000056 DAZKHC020000000
VERSION     DAZKHC020000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.056"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1024
                     /gene="fnlA"
                     /locus_tag="ACHOSM_RS14060"
                     /old_locus_tag="pgaptmp_002812"
     CDS             <1..1024
                     /gene="fnlA"
                     /locus_tag="ACHOSM_RS14060"
                     /old_locus_tag="pgaptmp_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     4,6-dehydratase/5-epimerase"
                     /protein_id="HGU4559297.1"
                     /translation="KTLLITGGTGSFGNAVLRRFLDTDIAEIRIFSRDEKKQDDMRKK
                     YNSDKLRFYIGDVRDFSSIRNASRGVDFIYHAAALKQVPSCEFHPMEAVKTNVLGTEN
                     VLEAAISNHVRKVVCLSTDKAVYPINAMGISKAMMEKVMVAKSRNVDSSKTVICGTRY
                     GNVMASRGSVIPLFVDLIKSGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNNGDIF
                     VQKAPAATIQTLAIALKELLNVHEHPVNVIGTRHGEKLYEALLSREEMIAAEDMGDYY
                     RVPPDLRDLNYGKYVEHGDRRISEVEDYNSHNTERLDVEGMKKLLLKLPFIRALRSGE
                     DYELDS"
     gene            1026..2132
                     /locus_tag="ACHOSM_RS14065"
                     /old_locus_tag="pgaptmp_002813"
     CDS             1026..2132
                     /locus_tag="ACHOSM_RS14065"
                     /old_locus_tag="pgaptmp_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HGU4559298.1"
                     /translation="MKILVTGAAGFIGRNLVFRLKEAGYNELITIVRNSSLADLEKGL
                     KQADFIFHLAGVNRPVKESEFEEGNSNLTQQIVDILKKNNKDTPIMLSSSIQAECDNA
                     YGKSKAAAEKIIQQYGETTNAKYYIYRLPNVFGKWCRPNYNSFIATFCHRIANDEAIT
                     INDPSAVVNLVYIDDFCSDILKLLEGANETGYRTFGPVYSVTVGEVAQLIYRFKESRQ
                     TLITEDVGNGFTRALYSTWLSYLSPEQFAYTVPSYSDDRGVFCEVLKTKNAGQFSFFT
                     AHPGITRGGHYHHSKNEKFIVIRGSACFKFENIVTGERYELNVSSDDFKIVETVPGWT
                     HDITNNGSDELVVMLWANEIFNRSEPDTIARVLS"
     gene            2129..3259
                     /gene="wecB"
                     /locus_tag="ACHOSM_RS14070"
                     /old_locus_tag="pgaptmp_002814"
     CDS             2129..3259
                     /gene="wecB"
                     /locus_tag="ACHOSM_RS14070"
                     /old_locus_tag="pgaptmp_002814"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGU4559299.1"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKLDEYCDHLIVHTGQNYDYE
                     LNEVFFKDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVLEQEKPEAMLVLGDTNSCI
                     SAIPAKRRRIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINMTYSDIAREYLLAEGV
                     PADRIIKTGSPMFEVLTHYMPQIDGSDVLSRLNLTPGNFFVVSAHREENVDTPKQLAK
                     LANILNTVAKKYDVPVVVSTHPRTRNRINENGIQFHKNILLLKPLGFHDYNHLQKNAR
                     AVLSDSGTITEESSIMNFPALNIREAHERPEGFEEGAVMMVGLESERVLQALEIIATQ
                     PRGKVRLLRQVSDYSMPNVSDKVVRIIHSYTDYVKRVVWKQY"
     gene            3259..4467
                     /locus_tag="ACHOSM_RS14075"
                     /old_locus_tag="pgaptmp_002815"
     CDS             3259..4467
                     /locus_tag="ACHOSM_RS14075"
                     /old_locus_tag="pgaptmp_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU4559300.1"
                     /translation="MKLALIIDDYLPHSTRVGAKMFHELGLELLSRGHDVTVITPDIT
                     LQAIYSVSMIDGIKVWRFKSGPLKDVGKAKRAINETLLSFRAWRAFKHLIQHDTFDGI
                     VYYSPSIFWGDLVKKIKQRCQCPSYLVLRDMFPQWVIDAGMLKAGSPIEKYFRYFEKK
                     SYQQADRIGLMSDKNLEIFRQTNKGYPCEVLRNWASMIPVSASDDYHSLRQKYDLKDK
                     VIFFYGGNIGHAQDMANLMRLARNMMRYHDAHFLFIGQGDEVDLIKSLAAEWSLTNFT
                     HLPSVNQEEFKLILSEVDVGLFSLSSRHSSHNFPGKLLGYMVQSIPILGSVNGGNDLM
                     DIINKHRAGFIHVNGEDDKLFESAQLLLSDSVLRKQLGQNANVLLKSQFSIESAAHTI
                     EVRLEAGECV"
     gene            4458..4865
                     /locus_tag="ACHOSM_RS14080"
                     /old_locus_tag="pgaptmp_002816"
     CDS             4458..4865
                     /locus_tag="ACHOSM_RS14080"
                     /old_locus_tag="pgaptmp_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HGU4559301.1"
                     /translation="MRLVDDNILDELFRTAANSERLRAHYLLHASHQEKVQRLLIAFV
                     RDSYVEPHWHELPHQWEMFVVMQGQLEVCLYEQNGEIQKKFVVGDGTGISVVEFSPGD
                     IHSVKCLSPKALMLEIKEGPFDPLKAKAFSKWL"
     gene            4977..6383
                     /gene="gndA"
                     /locus_tag="ACHOSM_RS14085"
                     /old_locus_tag="pgaptmp_002817"
     CDS             4977..6383
                     /gene="gndA"
                     /locus_tag="ACHOSM_RS14085"
                     /old_locus_tag="pgaptmp_002817"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGU4559302.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AAAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            6632..7798
                     /gene="ugd"
                     /locus_tag="ACHOSM_RS14090"
                     /old_locus_tag="pgaptmp_002818"
     CDS             6632..7798
                     /gene="ugd"
                     /locus_tag="ACHOSM_RS14090"
                     /old_locus_tag="pgaptmp_002818"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HGU4559303.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIQFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKRQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            7944..8921
                     /gene="wzzB"
                     /locus_tag="ACHOSM_RS14095"
                     /old_locus_tag="pgaptmp_002819"
     CDS             7944..8921
                     /gene="wzzB"
                     /locus_tag="ACHOSM_RS14095"
                     /old_locus_tag="pgaptmp_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HGU4559304.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIINQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLLLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9104..9715)
                     /gene="hisIE"
                     /locus_tag="ACHOSM_RS14100"
                     /old_locus_tag="pgaptmp_002820"
     CDS             complement(9104..9715)
                     /gene="hisIE"
                     /locus_tag="ACHOSM_RS14100"
                     /old_locus_tag="pgaptmp_002820"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HGU4559305.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMTPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(9709..10485)
                     /gene="hisF"
                     /locus_tag="ACHOSM_RS14105"
                     /old_locus_tag="pgaptmp_002821"
     CDS             complement(9709..10485)
                     /gene="hisF"
                     /locus_tag="ACHOSM_RS14105"
                     /old_locus_tag="pgaptmp_002821"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HGU4559306.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADQFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            complement(10467..11204)
                     /gene="hisA"
                     /locus_tag="ACHOSM_RS14110"
                     /old_locus_tag="pgaptmp_002822"
     CDS             complement(10467..11204)
                     /gene="hisA"
                     /locus_tag="ACHOSM_RS14110"
                     /old_locus_tag="pgaptmp_002822"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HGU4559307.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            complement(11204..11794)
                     /gene="hisH"
                     /locus_tag="ACHOSM_RS14115"
                     /old_locus_tag="pgaptmp_002823"
     CDS             complement(11204..11794)
                     /gene="hisH"
                     /locus_tag="ACHOSM_RS14115"
                     /old_locus_tag="pgaptmp_002823"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HGU4559308.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTVQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            complement(11794..12861)
                     /gene="hisB"
                     /locus_tag="ACHOSM_RS14120"
                     /old_locus_tag="pgaptmp_002824"
     CDS             complement(11794..12861)
                     /gene="hisB"
                     /locus_tag="ACHOSM_RS14120"
                     /old_locus_tag="pgaptmp_002824"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HGU4559309.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            complement(12861..13931)
                     /gene="hisC"
                     /locus_tag="ACHOSM_RS14125"
                     /old_locus_tag="pgaptmp_002825"
     CDS             complement(12861..13931)
                     /gene="hisC"
                     /locus_tag="ACHOSM_RS14125"
                     /old_locus_tag="pgaptmp_002825"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGU4559310.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLAHEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            complement(13928..15232)
                     /gene="hisD"
                     /locus_tag="ACHOSM_RS14130"
                     /old_locus_tag="pgaptmp_002826"
     CDS             complement(13928..15232)
                     /gene="hisD"
                     /locus_tag="ACHOSM_RS14130"
                     /old_locus_tag="pgaptmp_002826"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HGU4559311.1"
                     /translation="MSFNTIIDWNSCTTEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            complement(15238..16137)
                     /gene="hisG"
                     /locus_tag="ACHOSM_RS14135"
                     /old_locus_tag="pgaptmp_002827"
     CDS             complement(15238..16137)
                     /gene="hisG"
                     /locus_tag="ACHOSM_RS14135"
                     /old_locus_tag="pgaptmp_002827"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HGU4559312.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     misc_feature    complement(16184..16307)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(16283..16333)
                     /gene="hisL"
                     /locus_tag="ACHOSM_RS14140"
                     /old_locus_tag="pgaptmp_002828"
     CDS             complement(16283..16333)
                     /gene="hisL"
                     /locus_tag="ACHOSM_RS14140"
                     /old_locus_tag="pgaptmp_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HGU4559313.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            16616..16867
                     /gene="yefM"
                     /locus_tag="ACHOSM_RS14145"
                     /old_locus_tag="pgaptmp_002829"
     CDS             16616..16867
                     /gene="yefM"
                     /locus_tag="ACHOSM_RS14145"
                     /old_locus_tag="pgaptmp_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HGU4559314.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            16864..17118
                     /gene="yoeB"
                     /locus_tag="ACHOSM_RS14150"
                     /old_locus_tag="pgaptmp_002830"
     CDS             16864..17118
                     /gene="yoeB"
                     /locus_tag="ACHOSM_RS14150"
                     /old_locus_tag="pgaptmp_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HGU4559315.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            17201..18025
                     /gene="yeeZ"
                     /locus_tag="ACHOSM_RS14155"
                     /old_locus_tag="pgaptmp_002831"
     CDS             17201..18025
                     /gene="yeeZ"
                     /locus_tag="ACHOSM_RS14155"
                     /old_locus_tag="pgaptmp_002831"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4559316.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            18071..19000
                     /gene="yeeY"
                     /locus_tag="ACHOSM_RS14160"
                     /old_locus_tag="pgaptmp_002832"
     CDS             18071..19000
                     /gene="yeeY"
                     /locus_tag="ACHOSM_RS14160"
                     /old_locus_tag="pgaptmp_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4559317.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            19215..19277
                     /gene="yoeI"
                     /locus_tag="ACHOSM_RS14165"
                     /old_locus_tag="pgaptmp_002833"
     CDS             19215..19277
                     /gene="yoeI"
                     /locus_tag="ACHOSM_RS14165"
                     /old_locus_tag="pgaptmp_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HGU4559318.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            19267..20625
                     /gene="plaP"
                     /locus_tag="ACHOSM_RS14170"
                     /old_locus_tag="pgaptmp_002834"
     CDS             19267..20625
                     /gene="plaP"
                     /locus_tag="ACHOSM_RS14170"
                     /old_locus_tag="pgaptmp_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HGU4559319.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            20804..21862
                     /gene="tsuA"
                     /locus_tag="ACHOSM_RS14175"
                     /old_locus_tag="pgaptmp_002835"
     CDS             20804..21862
                     /gene="tsuA"
                     /locus_tag="ACHOSM_RS14175"
                     /old_locus_tag="pgaptmp_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HGU4559320.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFSYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            21876..22103
                     /gene="tsuB"
                     /locus_tag="ACHOSM_RS14180"
                     /old_locus_tag="pgaptmp_002836"
     CDS             21876..22103
                     /gene="tsuB"
                     /locus_tag="ACHOSM_RS14180"
                     /old_locus_tag="pgaptmp_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HGU4559321.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(22146..23570)
                     /gene="sbcB"
                     /locus_tag="ACHOSM_RS14185"
                     /old_locus_tag="pgaptmp_002837"
     CDS             complement(22146..23570)
                     /gene="sbcB"
                     /locus_tag="ACHOSM_RS14185"
                     /old_locus_tag="pgaptmp_002837"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HGU4559322.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGINPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENHNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPTDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            23752..24930
                     /locus_tag="ACHOSM_RS14190"
                     /old_locus_tag="pgaptmp_002838"
     CDS             23752..24930
                     /locus_tag="ACHOSM_RS14190"
                     /old_locus_tag="pgaptmp_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4559323.1"
                     /translation="MAISDTKLRSIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQIKPFLEKAMTTYNDVLFPGEDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTL
                     VTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD
                     "
     gene            complement(24911..25102)
                     /locus_tag="ACHOSM_RS14195"
                     /old_locus_tag="pgaptmp_002839"
     CDS             complement(24911..25102)
                     /locus_tag="ACHOSM_RS14195"
                     /old_locus_tag="pgaptmp_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4559324.1"
                     /translation="MTDTSLIPEKEVMNKLGVSSRQTIWNYTKRHGFPKPVRTHPKSY
                     LREAVEGWILNGGVNQKCS"
     gene            complement(25182..25526)
                     /locus_tag="ACHOSM_RS14200"
                     /old_locus_tag="pgaptmp_002840"
     CDS             complement(25182..25526)
                     /locus_tag="ACHOSM_RS14200"
                     /old_locus_tag="pgaptmp_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559325.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DVSDFRRCVALVDQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            complement(25553..25720)
                     /locus_tag="ACHOSM_RS14205"
                     /old_locus_tag="pgaptmp_002841"
     CDS             complement(25553..25720)
                     /locus_tag="ACHOSM_RS14205"
                     /old_locus_tag="pgaptmp_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559326.1"
                     /translation="MHFRVTGEWNGEPFDRVIEAEDINDCYNHWMIWAQIAHAEVTNI
                     QIEELKEHQTA"
     gene            complement(25793..26077)
                     /locus_tag="ACHOSM_RS14210"
                     /old_locus_tag="pgaptmp_002842"
     CDS             complement(25793..26077)
                     /locus_tag="ACHOSM_RS14210"
                     /old_locus_tag="pgaptmp_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGU4559327.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMLREYDRL
                     IITKGYPKRDDSSRRIDVPCDGYEIKTITHPHFGDKPVKVYAIKVNISNE"
     gene            complement(26070..26456)
                     /locus_tag="ACHOSM_RS14215"
                     /old_locus_tag="pgaptmp_002843"
     CDS             complement(26070..26456)
                     /locus_tag="ACHOSM_RS14215"
                     /old_locus_tag="pgaptmp_002843"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4559328.1"
                     /translation="MEVSIDVSTCDADLGNRYFGTVTEALELDTAKNGYILLVQDAEP
                     NFDVNGNSPATPDSWISCSERMPDTKTAVLVAVEFDRKGDWRMKWATYIPGHPDANDG
                     WIIPGASWIPSHWMPLPEPPQKVNRG"
     gene            complement(26493..26618)
                     /locus_tag="ACHOSM_RS14220"
                     /old_locus_tag="pgaptmp_002844"
                     /pseudo
     CDS             complement(26493..26618)
                     /locus_tag="ACHOSM_RS14220"
                     /old_locus_tag="pgaptmp_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26615..26824)
                     /locus_tag="ACHOSM_RS14225"
                     /old_locus_tag="pgaptmp_002845"
     CDS             complement(26615..26824)
                     /locus_tag="ACHOSM_RS14225"
                     /old_locus_tag="pgaptmp_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559329.1"
                     /translation="MAIAASYTMHLYCDCLQCTDGKYKSPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFAPGHKVLRINK"
     gene            complement(27187..27927)
                     /locus_tag="ACHOSM_RS14230"
                     /old_locus_tag="pgaptmp_002846"
     CDS             complement(27187..27927)
                     /locus_tag="ACHOSM_RS14230"
                     /old_locus_tag="pgaptmp_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4559330.1"
                     /translation="MNKIDYQALREAAEKAGEDKWQAKKINGDFFVIRHGSYTRQHGY
                     TSYQPIAEIDCKPVRDFVAKANPATVLALLDERERNQQYIKRRDQENEEIALTVGKLR
                     VELEAAENNLIDSECHVAELEEALRDKQALLEASEKRNAKLQSENAYIRNRYKELDLL
                     IGKNILVMQAAIIEWQATGDAKSGLAWIYNTLFGPGELPDESEKDAQAYFNRKYAPID
                     EKLMALHKWFWEQSEAERAAGIRIKGGE"
     gene            complement(27929..28228)
                     /locus_tag="ACHOSM_RS14235"
                     /old_locus_tag="pgaptmp_002847"
     CDS             complement(27929..28228)
                     /locus_tag="ACHOSM_RS14235"
                     /old_locus_tag="pgaptmp_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559331.1"
                     /translation="MSVYLIDKRRRGQQIPPVGIPNHTWFCVLDIDGMDSLVDTRHYC
                     DTATATPAKAKKMADLIENWTPPDGWCNGNDRDWHEKMKGYICDFLRKCNGFRVM"
     gene            complement(28225..28587)
                     /locus_tag="ACHOSM_RS14240"
                     /old_locus_tag="pgaptmp_002848"
     CDS             complement(28225..28587)
                     /locus_tag="ACHOSM_RS14240"
                     /old_locus_tag="pgaptmp_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559332.1"
                     /translation="MVRKFGELESKLETALRECRSAGITIDNLEAKCVALAAESAGMK
                     KFCKDAAFDADYEAELGMERGGFSDALNEIKTPATDAFLAEVRAQGVEMFAECAYTLE
                     HHDHAVAFAAELRKGGNQ"
BASE COUNT         7077 a         6798 c         7008 g         7706 t
ORIGIN      
        1 taaaacactt ttaattacag gcggtactgg ttcgtttgga aatgctgttt tacgtcgatt
       61 tttagacact gacattgctg agatccgtat tttcagtcgt gatgaaaaaa aacaagatga
      121 tatgcggaaa aaatataata gtgataaatt gaggttttat attggagatg ttagggactt
      181 tagcagtata cggaatgcaa gtagaggtgt tgatttcatt tatcatgcag cagctcttaa
      241 gcaagtacca tcatgtgagt ttcacccaat ggaggctgtt aaaactaatg ttttaggtac
      301 tgaaaatgtt ctcgaagctg ctatatcaaa tcatgtgcgt aaagttgttt gcttaagtac
      361 ggacaaagct gtttatccta tcaatgctat gggtatttca aaagccatga tggaaaaagt
      421 tatggtagct aaatcgcgta atgttgatag ctccaaaaca gtcatatgcg gaactcgtta
      481 tggcaacgtt atggcttcac gtggatcggt cattccatta tttgttgatc taatcaaatc
      541 tggtaaacca ttgaccatta ccgatcccaa tatgactcgt ttcatgatga cgcttgagga
      601 tgctgttgat ctggtccttt atgccttcga gcatggaaat aacggtgaca ttttcgttca
      661 gaaagcacct gcggcaacaa ttcaaacatt agccattgca cttaaggaat tgctaaatgt
      721 ccatgaacat ccagtcaatg ttattggaac tcgacacggg gaaaaacttt acgaagcatt
      781 attgagccga gaggaaatga ttgcagcgga agatatgggt gattattatc gtgttccacc
      841 agatctccgc gatttgaact atggaaaata tgtggaacat ggtgaccgtc gtatctcgga
      901 agtggaagat tataactctc ataatactga gagattagat gttgagggaa tgaaaaaatt
      961 actgctaaaa cttcctttta tccgggcact tcgttccggt gaagattatg agttggattc
     1021 ataatatgaa aattttagtt actggcgcgg cagggtttat cggtcgaaat ttggttttcc
     1081 gccttaagga agctggatat aacgaactta ttacgatagt tcgtaactct tctttggcgg
     1141 atttagaaaa gggacttaag caggcagatt tcatttttca ccttgctgga gtaaatcgtc
     1201 ctgtgaagga gagtgaattt gaagagggga atagcaatct aactcaacag attgttgata
     1261 ttttgaaaaa aaataataaa gatactccta tcatgcttag ttcttccatc caggctgaat
     1321 gtgataacgc ttatggaaag agtaaagcag ctgcggaaaa aatcattcag cagtacgggg
     1381 aaacgacaaa tgctaaatat tatatttatc gcttgccgaa tgtattcggt aagtggtgtc
     1441 gaccaaatta taactccttt atagcaactt tctgccatcg cattgcaaat gatgaagcta
     1501 ttacaattaa tgatccttca gcagttgtaa atctggtgta tatagatgat ttttgttctg
     1561 acattttaaa gctattagaa ggagcgaacg aaactggtta caggacattt ggtccagttt
     1621 attctgttac tgttggtgaa gtggcacaat taatttatcg atttaaagaa agccgccaaa
     1681 cattaatcac cgaagatgta ggtaatggat ttacacgtgc attgtactca acatggttaa
     1741 gttacctttc tcctgaacag tttgcgtata cggttccttc ttatagtgat gacagagggg
     1801 tattctgtga agtattgaaa acgaaaaacg cgggtcagtt ttcttttttt actgcgcatc
     1861 caggaattac tcggggtggt cattatcatc attccaaaaa tgagaaattt attgtcatcc
     1921 gaggaagtgc ttgtttcaaa tttgaaaata ttgtcacggg tgaacgatat gaacttaatg
     1981 tttcatcaga tgattttaaa attgttgaaa cagttccggg atggacgcat gacattacta
     2041 ataatggctc ggatgagcta gttgttatgc tttgggcaaa tgaaatattt aatcgttctg
     2101 aaccagatac tatagcgaga gttttatcgt gaaaaaattg aaagtcatgt cggttgttgg
     2161 gactcgtcca gaaattattc gactctcgcg tgtccttgca aaattagatg aatattgtga
     2221 tcaccttatt gttcatactg gtcaaaacta cgattatgaa ttgaatgagg tttttttcaa
     2281 agatttgggt gttcgcaaac ctgattattt tcttaatgcc gccggtaaaa atgcagcaga
     2341 gacaattgga caagttatca ttaaagttga tgaggttctt gaacaggaaa aaccagaagc
     2401 catgttagtt cttggtgata ctaactcctg tatttcagca ataccagcaa agcgtcgaag
     2461 aattccgatc ttccatatgg aggcggggaa tcgttgtttt gaccaacgcg taccggaaga
     2521 aactaacaga aaaatagttg accataccgc tgatatcaat atgacatata gtgatatcgc
     2581 gcgtgaatat cttctggctg aaggtgtacc agccgataga attattaaaa ccggtagccc
     2641 aatgtttgaa gtactcactc attatatgcc gcagattgat ggttccgatg tactttctcg
     2701 cctgaattta acacctggga atttctttgt ggtaagtgcc cacagagaag aaaatgttga
     2761 tactcctaaa cagcttgcga aactggcgaa tatacttaat accgtagcta aaaaatatga
     2821 tgtcccggta gtcgtttcta ctcatcctcg cactcgtaac cgcatcaacg aaaacggtat
     2881 tcaattccat aaaaatatct tgcttcttaa gccattagga tttcacgatt ataaccatct
     2941 gcaaaaaaat gcacgtgctg ttttatcgga tagtgggact attacagaag agtcctccat
     3001 tatgaacttc cctgcactca atatacgaga agcgcacgaa cgcccggaag gcttcgaaga
     3061 aggggcagta atgatggtcg gtcttgaatc tgagcgcgtc ttacaggcat tagaaattat
     3121 tgcaacacag cctcgtggaa aagtacgctt acttcgtcag gtcagtgact atagcatgcc
     3181 aaatgtttca gataaagttg tgcgtattat ccattcatac actgactacg ttaaacgggt
     3241 tgtctggaag caatactaat gaaacttgca ttaatcattg atgattattt gccccacagc
     3301 acacgtgttg gggctaaaat gtttcatgag ttaggccttg aattgctgag cagaggccat
     3361 gatgtaactg taattacgcc tgacatcaca ttacaagcaa tctattctgt tagtatgatt
     3421 gatggtataa aggtttggcg tttcaaaagt ggacctttaa aggatgtagg taaggctaaa
     3481 cgagccataa atgaaactct tttatctttt cgtgcatggc gcgcatttaa gcacctcatt
     3541 cagcatgata catttgatgg tatcgtttat tattccccct ctattttttg gggagacttg
     3601 gttaaaaaaa taaaacagcg atgccagtgc ccaagctatc tggtcctaag ggatatgttt
     3661 ccacagtggg ttattgatgc aggtatgttg aaagccggtt cgccaattga aaaatatttc
     3721 aggtattttg aaaaaaaatc atatcagcag gctgaccgga tagggttaat gtctgataag
     3781 aatcttgaga tatttcgtca gaccaataaa ggttatccgt gtgaagtttt acgtaattgg
     3841 gcctcaatga ttcctgtgtc tgccagcgat gattatcatt cacttcgtca aaaatacgat
     3901 ctaaaagata aagttatttt tttctatggc ggtaatattg ggcatgctca agatatggca
     3961 aacttaatgc gccttgcgcg taatatgatg cgttatcatg atgctcattt cctgtttatt
     4021 gggcagggtg atgaagttga cctgataaaa tctcttgctg cagaatggag tttaactaat
     4081 ttcactcatc taccttcagt gaaccaggaa gagtttaaat taattttatc tgaagttgat
     4141 gtcggcctat tctccctttc atctcgccat tcttcacata atttccccgg gaaattacta
     4201 gggtatatgg ttcaatcaat cccgatcctt gggagtgtga atggcggcaa tgatttaatg
     4261 gatataatta ataagcacag ggccggtttt attcatgtta atggtgaaga tgataaactg
     4321 tttgaatctg cacaattgct tcttagtgat tcagttttaa gaaaacagtt aggtcagaac
     4381 gctaatgtgt tgttaaagtc tcaattttcg attgaatcgg cggcacatac tatcgaagtc
     4441 cgactggagg caggagaatg cgtttagttg atgacaatat tctggatgaa ctttttcgca
     4501 ctgcagcaaa ttctgaacgt ttgcgcgctc attatttatt gcacgcatct catcaggaga
     4561 aggttcaacg tttacttatt gcatttgtac gcgacagtta tgttgaaccc cattggcatg
     4621 agttaccgca tcagtgggaa atgtttgtcg tcatgcaagg gcaattagaa gtttgtttgt
     4681 atgagcaaaa tggtgagatc caaaaaaaat ttgttgttgg agacggtacg ggaataagcg
     4741 tcgtggaatt ttccccagga gatatacata gtgtcaaatg cctgtcacca aaagccctta
     4801 tgttagagat aaaggagggg ccatttgacc cactgaaagc taaggctttt tctaagtggt
     4861 tatagggcga tacaccaccg tttattcttc tattttattc tatacatgct gggttaccat
     4921 cttagcttct tcaagccgcg caaccccgcg gtgatcaccc ctgacaggag taaacaatgt
     4981 caaagcaaca gatcggcgtc gtcggtatgg cagtgatggg acgcaacctt gcgctcaaca
     5041 tcgaaagccg tggttatacc gtctctattt tcaaccgttc ccgtgaaaag accgaagaag
     5101 tgattgccga aaatccaggc aagaaactgg ttccttacta tacggtgaaa gagtttgttg
     5161 aatctctgga aacgcctcgt cgcatcctgt taatggtgaa agcaggtgca ggcacggatg
     5221 ctgctattga ttccctcaaa ccatatctcg ataaaggtga catcatcatt gatggtggta
     5281 acaccttctt ccaggacacc attcgtcgta accgtgagct ttctgccgaa ggctttaact
     5341 tcattggtac cggtgtctcc ggtggtgaag aaggcgcgct gaaaggtcct tccattatgc
     5401 ctggtgggca gaaagaagcc tatgaactgg ttgcgccgat cctgactaaa atcgccgcag
     5461 cggctgaaga cggtgagcca tgtgttacct atatcggtgc cgatggtgca ggccattatg
     5521 tgaagatggt tcacaacggt attgaatacg gagatatgca actgattgct gaagcctatt
     5581 ctctgcttaa aggtggcctg aacctcacca acgaagaact ggcgcagacg tttaccgagt
     5641 ggaataacgg tgaactgagc agctacctga tcgacatcac caaagatatc ttcaccaaaa
     5701 aagatgaaga cggtaactac ctggttgatg tgatcctgga tgaagcggct aacaaaggta
     5761 ccggtaaatg gaccagccag agcgcactgg atctcggcga accgctgtcg ctgattaccg
     5821 agtctgtgtt tgcacgttat atctcttctc tgaaagatca gcgtgttgcc gcatctaaag
     5881 ttctctctgg cccgcaagca cagccagcag gcgacaaggc tgagttcatc gaaaaagttc
     5941 gccgtgcgct gtatctgggc aaaatcgttt cttacgccca gggcttctct cagctgcgtg
     6001 ctgcgtctga agagtacaac tgggatctga actacggcga aatcgcgaag attttccgtg
     6061 ctggctgcat cattcgtgcg cagttcctgc agaaaattac cgatgcttat gccgaaaatc
     6121 cgcagatcgc taacctgctg ctggctccgt acttcaagca aattgccgat gactaccagc
     6181 aggcgctgcg cgatgtcgtc gcttatgcag tacagaacgg tatcccggtt ccgaccttcg
     6241 ccgctgcggt tgcctattat gacagctacc gtgccgctgt tctgcctgca aacctgatcc
     6301 aggcacagcg tgactatttc ggtgcgcata cttataagcg cattgataaa gaaggtgtgt
     6361 tccataccga atggctggat taatctgatt taaccaacaa taaaattgag gcccggcgta
     6421 tattgcaccg ggcttttttt tgccaattat cttatagact aaatttcact gcttaatatt
     6481 aacttaataa atatcagcta ttcttataaa gaaaatctga attgtttttc gttgcgttga
     6541 ccatcgaaca acgtagcgct aaaactttta gctcttatca cgatgctaaa aacatcatga
     6601 tttacagtta agttaattct gagagcatga aatgaaaatc accatttccg gtactggcta
     6661 tgttggcttg tcaaatggcc ttctaattgc acaaaatcat gaggttgtgg cattagatat
     6721 tttaccgtca cgcgttgcta tgctgaatga tcggatatct cctattgttg ataaggaaat
     6781 tcagcagttt ttgcaatcag ataaaataca atttaatgcc acattagata aaaatgaagc
     6841 ctaccgggat gctgattatg tcatcatcgc cactccaacc gactatgatc ctaaaactaa
     6901 ttatttcaat acgtccagtg tagaatcagt aattaaagac gtagtagaga taaatcctta
     6961 tgcggttatg gtcatcaaat caacggttcc cgttggtttt accgcagcga tgcataagaa
     7021 atatcgcact gaaaatatta tattctcccc ggaatttctc cgtgagggta aagcccttta
     7081 cgataatctc catccttcac gtattgtcat cggtgagcgt tcagaacgcg cagaacgttt
     7141 tgctgctctg ttacaggaag gcgcgattaa gcaaaatatc ccgaccctgt ttaccgactc
     7201 cactgaagca gaagcgatta aactttttgc aaacacctac ctggcgatgc gcgtggcgta
     7261 ctttaacgaa ctggatagct atgcagaaag tttaggtctg aattcccgtc aaataatcga
     7321 aggcgtttgt ctcgacccac gtattggcaa ccattacaac aatccgtcgt ttggttatgg
     7381 tggttattgt ctgccgaaag ataccaagca gttactggcg aactaccagt ctgtgccgaa
     7441 taacctgatc tctgcaattg tcgatgctaa ccgcacgcgt aaagatttta ttgccgatgc
     7501 cattttgtca cgcaagccgc aagtggtggg tatttatcgt ctgattatga agagcggttc
     7561 agacaacttc cgcgcgtctt ccattcaggg gattatgaaa cgtatcaagg cgaaaggcgt
     7621 tgaagtgatc atttacgagc cggtgatgaa agaagactca ttcttcaact ctcgcctgga
     7681 acgcgatctc gccaccttca aacgacaagc tgacgtcatt atttccaacc gtatggcaga
     7741 agagcttaaa gatgtggcag ataaggtcta cacccgcgat ctctttggca gcgactaaca
     7801 tcctgttatc agggctattt acgccctgat tgtcttttgt tacttgcgta acaattcatt
     7861 atttttatca attatcctat agcattcatg aggattatcg ctaaactatg cggacttgga
     7921 aatttccgtc agttagggta atgatgagag tagaaaataa taatgtttct gggcaaaacc
     7981 atgacccgga acagattgat ttgattgatt tactagtgca gttgtggcgt ggcaagatga
     8041 caatcatcat ttccgtcatt gtggctattg ccctggctat tggatatttg gcagtagcga
     8101 aggagaaatg gacgtcaaca gcaattatca atcagcccga tgtggggcaa attgctggct
     8161 ataacaatgc catgaatgtt atctatggtc aggctgcacc gaaagtatcg gatttgcagg
     8221 agacgttaat tggtcgcttc agttctgcct tctctgcatt agcagaaacg ctggataatc
     8281 aggaagaacc agaaaaactt accatcgaac cttctgttaa gaaccagcaa ttactattga
     8341 ctgtttctta tgttgggcaa actgcagagg gcgcacaaat gaagttggcc caatacattc
     8401 agcaagttga cgataaagtg aatcaagagc tagaaaagga tctcaaggac aacattgctc
     8461 tgggacggaa aaacttgcag gactctttaa gaacgcagga agtggttgcg caggagcaga
     8521 aagatctgcg tatccgtcag attcaggaag cgttgcagta tgcgaatcag gcgcaggtga
     8581 caaagccaca ggttcagcag actgaagatg tgacgcaaga tacgttgttc cttttgggga
     8641 gcgaagcgct ggagtcgatg attaagcatg aggcgacccg tccgttggtg ttctcaccaa
     8701 actactatca gactcgtcaa aacctgctgg atatcgaaag cttaaaagtt gatgatcttg
     8761 atattcatgc ttaccgttat gtaatgaaac cgacgttacc tattcgtcgc gatagcccga
     8821 aaaaggcaat taccttgatt ttggctgtgc tgctgggcgg catggttggt gcggggattg
     8881 tgttggggcg taatgctcta cgtaattaca acgcgaagta atcttttcgg ttttaaagaa
     8941 aaagggcagg gtggtgacac cttgcccgtt ttttttgccg gatgcgatgc tggcgcatct
     9001 tatccagcca aaatgtgcag agcgaaatgt aggcccgata agcttgcgca tcgggcaatg
     9061 gctccggatg cgactacaac atcgcatccg gtcagtccgc aactcactga tgccgtttgc
     9121 gcaggttctc aatcaccgtc gttaaatcca aatcctgatc ctgtaacagc accagcaggt
     9181 gatacatcaa gtcagatgcc tcgttggtca gctcaaagcg gtcatgtacc gttgctgcca
     9241 gtgcggtttc caccccttct tcgcccactt tctgcgcaat gcgcttggtg ccgctggcat
     9301 acagtttggc agtgtaagag gtttccgggt cggcagattt acgttcggcg agcagttgtt
     9361 ccagttgata caggaacagc cactggtgag cggtgtcgcc gaagcagctg ctggtgcctt
     9421 tatggcaggt tggtccgata ggattcgcca gtaccagtag cgtgtcgttg tcgcagtccg
     9481 gcgcaatgct cactacgttg aggaaattgc ccgacgtttc gcctttggtc cacagtcgct
     9541 gtttagtgcg cgagaagaag gtgactttgc cgctttcgat ggttttgtct aaggcttccg
     9601 gggtcatgta gcccagcatt aacacttcgc cggataccgc gtgttgcaca atcaccggca
     9661 tcagtccgtc ggttttttcc cagtccagtt cgcgacgttg ttgttctgtt aacatatcct
     9721 gatctccacg ccctgtgttg ccaggtacgc ttttaattca ccaatattga ttatttgttt
     9781 gtggaataca gaagctgcca gcgcgccgtc aacgtcggca tcgcggaagg cttcgaggaa
     9841 gtgttccatg gtccccgcgc caccggaggc aattagcggg acgtggcaaa cttcacgtac
     9901 ttttttcagt tgttcgaggt cgtaaccgtt acgcacgccg tcctgattca tcatgttgag
     9961 gacgatttct ccggcaccgc gtttttgcac ttcctgtacc cagtcgagcg tttcccactg
    10021 agtgacgcgg gtgcggcttt catcgccggt atattgattc acatgatatt taccggtttc
    10081 agcgtcgtac caggtatcaa taccgaccac aatacactgc acgccaaatt gatccgccag
    10141 gcgagtaatt aacgtcgggt ccgccagcgc aggcgagttt atggaaattt tatccgcgcc
    10201 aaaggaaaga attttcgcgg catcttccag agacttaatc ccacctgcca cacagaacgg
    10261 aatgtcgatc acttccgcca cgcgagatac ccagctttta tctaccacac ggccatcgct
    10321 ggaagcggtg atatcgtaga acaccagttc gtctgcacct tcttcggcgt agcgttttgc
    10381 cagcggtacg atatcgccaa tgatttcatg gttgcgaaac tgtacgcctt tcaccacctg
    10441 accatcacga acgtcgagac acggaattat gcgttttgcc agcatgcgat ggcctccttc
    10501 acggtgaatt taccttccag taatgcgcga ccgacaatta cgccgcgcac gccagtgcca
    10561 cgcagggcag caacatcgtt aatgtcgcca atgccgccgg aggactgaaa tgccacttgc
    10621 ggatatctgg cgcacacttc ttcatataaa gagacgttag aacctgccag cgtgccgtcg
    10681 cgcgagatat cggtacacag cacatgtttc aggccgacgg gcagataggt ttccaccagt
    10741 tgttccagcg acacacccga attctcttgc cagccgctga ccgccacctg cttgttgcct
    10801 tgctcgtcaa tacggacatc cagcgccagc actaaggcat cggcaccgaa gcgttcaaac
    10861 cagcctttca ccatatctgg tgatttcacc gcggtggagc caaccaccac acgcgcaacg
    10921 cccgcttcca gtaacgccgc cacgtcttct tcgctacgca cgccgccgcc aacctgcacc
    10981 ggaacgttaa cgcccgccac cagggtttta atcagtggga tttgacgttt agccggatct
    11041 tttgccccgg tcagatccac cagatgcaac acttcggcac cctgcgcggc gtaatcctgt
    11101 aagcgcggca gcgggtcgtt accgtaatcg cgctgtttgc cgtaatcgcc ctgatggaga
    11161 cgcaccacgg tgccgtcgat taaatctaat gccggaataa tcatcacatc tccaggaagt
    11221 ttttcagcaa tttagcgcca gcggcaccag aacgctccgg gtggaactgc acgccgtaga
    11281 agttatcttt ttgtaccgcc gcggtgaacg gttcgccgta attacactgg gcgatagtcc
    11341 atggattgac cggcattgcg taactgtgaa caaagtaaaa gtacgcgccg tcttcaatcc
    11401 cctgaaacag gcggttgcct gcctgcgggt aaacacggtt ccagcccata tgcggcagtg
    11461 gcagaccaaa gtcggtcatt ttcggcacgt cttcgtcgat gatgcccagc aaatcgacgc
    11521 cgttgctctc ttcgctgcgc cgccccagca gttgcatccc taagcagatg cccagcaccg
    11581 gttgggtaca ggctttgatg agatcaaaca gctcgcgctc acgcacctga tccatcgctg
    11641 cttgcacggt gccaacgccg ggtaaaaaca gtttatcggc cagcaacacg acgtccgggt
    11701 cacggctgac tttgggttca taaccgtgac gcgcaatggc agacttcacc gagttcaaat
    11761 tggcgcagcc agtatcaagg atcaccacgt tcattacagc actcctttcg acgagggcag
    11821 ggtgtcgcct tccacgcgga tggcctggcg cagggtgcga ccaaaggctt tgaacaggct
    11881 ctctacacgg tggtgatcgt ttttaccttt ggttttcagg tgtagcgtca cgcccatggt
    11941 gtatgagagc gaacggaaga agtgctcgat catttcggtg ctgagatcgc ccacgcgctg
    12001 gtaggtaaac tcggctttat attccaggtg cgggcgacca gagatatcca gcgcgcagcg
    12061 ggcaaggcat tcgtccatcg gtagcacaaa accaaagcgg caaataccgc gtttgtctcc
    12121 gagggcgatt tttagcgctt cgcccagcgc caggccggta tcttcgacgg tgtggtgatc
    12181 gtcgatatag aggtcgcctt tgacgttgat ttccatgcgg aaaccgccgt gggtggcgat
    12241 ctgatccagc atgtgatcaa agaagccaac accggtgtta atcttgctgc caccttcgcg
    12301 atccagccac acctggacgt caatttgcgt ctctttggtg ttgcgcacta catgggcgta
    12361 acggtctcgt ttagtgagtt gctcgccaat catcggccag ttcaggattt cgcggtcgta
    12421 gcgtaaacca ttaatgccca tgttttccgc cagttgaatg tcggtcgcgc gatcgccaat
    12481 cacataactg ttggcacggt ccatcgcttg ctcagccaga taacgctcca ccagttttac
    12541 tttcggctta cggcagtcgc actcatcggc gggcaggtga gggcaaatca gcacttcatc
    12601 aaactgcacg ccctgtgagg tgaagatctg catcatcagg ttgtgcgggc catcgaaatc
    12661 cgcctgcggg aaactttgtg ttcccagacc atcctgatta gtgatcatca ccagcttgta
    12721 gcccgctttt tgcagcttca gcagttccgg gatcacgccc ggttcaaagg cgagtttgtc
    12781 aaaacggtcc acctgaaaat cactcggcgg ttcgctaatc agggttccat cgcgatcgat
    12841 aaaaagatac ttctgactca tcaaacttgc tccgcacgta aggcgtcaat gacgcgctgg
    12901 ctttcttcac gggttccgac ggtaattcgc aggcagccgc ttaaagaggg ttgtttattc
    12961 tgatcacgta agataatgcc ctgatcccac aaagatttaa acactgcgct ggaggcttta
    13021 aagcgcgcca gaatgtagtt ggtttcggag tcgaaaacct gctctacgca ggggatctct
    13081 ttcagtgcgg caatcaggta ttcgcgttct gcaataattt gcgtcactcg ttcgcgcata
    13141 gcgacgattc cctgcgggct taacgcctgg gccgcaatgt cggcaaccgg cgtcgagagc
    13201 gggtaggggg cgatcacttt catcagcagg ttgatgactt cttcgtgtgc cagcgtaaac
    13261 ccgcaacgaa gccccgccag agcaaaagct ttcgacagtg tgcgtaaaat agccaggtgc
    13321 ggatattccg ccagccagcc agccagcgat gcctgtgggc aaaactcgat ataggcttca
    13381 tcggcaacca caatcgcctt accgcgcgtt aactccagca gggtgcgaaa atcctgcgga
    13441 ttgatcagtt gcccggttgg gttgttgggg ctgcaaacat agaccacttt tacgccgtcc
    13501 agcttgtcgg aaatgccctg taagtccagt tgccagtttt ccagcgtcgg cactgtgcgg
    13561 cactcgacgc caatggtttc agcgctgacg ctgtacatgc cgtacgttgg cgggcagtag
    13621 aggatggcgt ctttacccgg ttcgcaaaaa gcgcgaatca gtagttcaat accttcgtcc
    13681 gcgccacggc tgaccagcac ctgttccggt tttacgcctg catattgcgc gtaattttca
    13741 atcactgctt tcggctgaca ttccgggtag cggttgagcg tttgctgagt aagctgaaac
    13801 tccacggctg tgggatattc gttggcgttc agccagacgt cgccattacc gcccagacga
    13861 cgcgccgact gatacggcgt caggttgcgg acgttttcac gcgctaaatc ggtaatagtc
    13921 acggtgctca tgcttgctcc ttaagggcgt taacacgcaa agtaacggca tttttatggg
    13981 cggtcaggcg ctcggcggcg gccagtgttt ctatggtcga agccagcgca gaaaagcctt
    14041 ctttcgacag ttcctgcacg gtcatgcgct tctggaaatc tgccagcccg aggctggaac
    14101 aggtggcggt gtaaccgtaa gtcggtagaa cgtggttggt gccggaggcg taatcacctg
    14161 ccgattccgg tgaccagtca ccaagaaata ccgaaccggc gctggtgatg ctatcgacca
    14221 gatcgcgagc gttgcgggtc tgaatgatca ggtgctccgg gccgtactgg ttggagatct
    14281 ctacgcactg tgctaaatcg ttggtcacga tcaggcggct ggcgttcagt gcctggcggg
    14341 cggtttcggc acgcggcagt tctgccaact ggcgttcgac ggcctcggca acgcgacgcg
    14401 ccatatcagc agcgggcgtc agtaaaatca cctgtgagtc cgggccgtgt tcagcctggg
    14461 agagcaaatc agaagccacg aaatccggcg tagcgccgct gtcagcaatg accaacactt
    14521 ccgacgggcc tgcgggcata tcgatagccg caccgtccag acgctggctc acctgacgtt
    14581 tcgcttcggt gacaaaggcg ttacccggcc cgaagatttt gtccactttc ggcacagatt
    14641 ccgtaccaaa cgccagtgcg gcaatggcct gtgcgccgcc gacgttaaac acgtcctgta
    14701 caccgcacag ctgcgccgcg taaaggatct catcggcaat cggcggcggt gagcacagca
    14761 ccactttttt acagcccgca atacgcgccg gagttgccag cattaatacc gttgagaaga
    14821 gcggggcgga gccgccagga atatacaacc caactgaagc gactgggcgc gtgacctgct
    14881 ggcaacgcac gcctggctgg gtttctacat ctaccggcgg cagtttttgc gcagtgtgga
    14941 aggtttcaat attttttact gccaccgcca tcgcctgttt tagctcgtcg ctaaggcgtt
    15001 cgctggcggc ggcgatctcc tcagcagaca ccttcagcgc ggtaaccgtg gttttatcaa
    15061 acttcgcgct gtattcccgc agggcgtcat cgccgcgtgc tttcacgtta tcgagaatat
    15121 cgttaacggt gcgggtaatg ctttcagaag cggagatcgc cgggcgcatt aacagctggc
    15181 gttgttgctc cgtagtacag ctattccagt caatgattgt gttaaagctc atggcgatca
    15241 ctccatcatc ttctcaatcg gcaggaccag gattgaactg gcacccagcg ctttcagttt
    15301 ttccatcgtt tcccagaaca gggtttcgct gctgaccatg tgcatcgcta cgcgttgttg
    15361 gtcacccgcc agcggcagaa tggttgggcg ttcggcacct ggcagcaggg cgatgacttc
    15421 atccagacgt tcggtcggtg cgtgcatcat gatgtatttt gattcacgcg cctggataac
    15481 accctgaata cgggtcagca gtttgtcgat cagttgctgt ttggattctt ccatttcgcc
    15541 atcgcgttgt atcaggcagg ctttcgagcg atagataact tcgacttcgc gcaggccgtt
    15601 agcttccagc gtggcaccgg tggaaaccag atcgcaaatc gcatccgcca gtccggcacg
    15661 cggggcgact tcaacagaac cgttcagtaa gcaggattta aaagagatac ctttctggtc
    15721 gagataacgc ttgagcaggt gaggataaga ggtggcgata cgtttaccgt ttaaggagag
    15781 cgggccatcc caggcttcat caaccggcgt tgccagtgaa agtcggcagc cgccgaaatc
    15841 cagacgacgc agggtaaagt agcgtggatc ttcaccctgg gcgcggcggt taagcagctc
    15901 ttcttccagc acgttttcgc cgataatccc gaggtcaacc acgccgtcca ttaccagacc
    15961 gggaatgtcg tcgtcacgca cgcgcagaat atcaatcggc atgttttctg ccatcgcgat
    16021 caggcgctgg gtgtgaagat tgattttaat gccacagcgc gccagcaatt cgcgtgagtc
    16081 atcacttaaa cggccggatt tctgcatagc tatgcgtaaa cgagtgttgt ctgtcatttt
    16141 cttattcctc tttaaacctg tctgaaccgt tatcaaccgc gcaataaaaa aacccccgga
    16201 aggtgatctt ccgggggctt tctcatgcgt tcatgcacca ctggaagatc tgaatgtctt
    16261 ccagcacaca tcgcctgaaa gactagtcag gatgatggtg atgatggtgg tgtttaaatt
    16321 gaacgcgtgt cataaaagtc tctgtgaatg tttattcaac tgatgtcttt taacctaaac
    16381 cactttcacg ttagaaagca agggcttttt tatatatttt atggcgatgg atttgtttaa
    16441 tgaatgagcg gggaaaggag ggggctaaga ggactgtcct ctaagctgac ggcaccaaaa
    16501 atagcgaagc ctggattttc gtctcactga caataccttg atctacaaac taattaataa
    16561 atagttaatt aacgcccatc attgtacaat gaactgtaca aaagaggaga ttgacatgcg
    16621 tacaattagc tacagcgaag cgcgtcagaa tttgtcggca acaatgatga aagccgttga
    16681 agatcatgcc ccgatcctta ttactcgtca gaatggagag gcttgtgttc tgatgtcact
    16741 cgaagaatac aactcgctgg aagagacggc ttatctactg cgctcccccg ctaacgcccg
    16801 gagattgatg gactcaatcg atagcctgaa atcaggcaaa ggaacagaaa aggacattat
    16861 tgagtgaaac taatctggtc tgaggaatca tgggatgatt atctgtactg gcaggaaaca
    16921 gataagcgaa ttgttaaaaa gatcaatgaa cttatcaaag atacccgcag aacgccattt
    16981 gaaggtaagg ggaagccaga acccctgaaa cataatttgt caggtttctg gtcccgacgc
    17041 attacagagg agcaccgtct ggtatacgcg gttaccgacg attcactgct cattgcagca
    17101 tgtcgttatc attattgaac agattcattc atagaatgaa ttgtcagttt tgatacgcta
    17161 gcgtagcctt atagatctaa ggtgtatcag gagataacgg atgaaaaagg tcgcaattgt
    17221 cgggttaggg tggttaggca tgccgctggc gatgtcactt tcagcgcgag gctggcaagt
    17281 caccgggagt aaaaccacac aagatggtgt cgaagcggcc cgaatgagtg gcattgatag
    17341 ctatctgctt cgcatggagc ctgagctagt ttgcgattct gacgatctgg atgccctgat
    17401 ggatgccgat gcgctggtca ttacgcttcc ggcacgtcgt agcggccccg gcgatgagtt
    17461 ctatttacaa gcggtacaag agttagtgga tagcgcgctg gcccatcgta ttccccgcat
    17521 tatttttacc agctcaacat ctgtctatgg cgacgcgcaa ggcacggtga aagaaaccac
    17581 cccgcgtaat ccagtaacca acagtggacg agtattagaa gaactcgaag actggctgca
    17641 caatttaccc ggtacttcgg tcgatattct gcgtcttgcg ggcctggtcg gaccgggacg
    17701 tcatcccgga cgcttctttg ccggaaaaac cgcgcctgat ggtgaacatg gtgttaattt
    17761 agtccattta gaagatgtta ttggcgctat cactctgttg ttacaggcac ctaaaggcgg
    17821 acacatctat aatatatgtg cgccagctca ccctgcgcgt aatgttttct atccgcagat
    17881 ggcccgttta ctggggctgg aacctccgca gttcagaaat agtctggaca gcggcaaagg
    17941 taagattatt gatggcagtc ggatttgtaa tgaactggga tttgaatacc agtatcccga
    18001 tccgctggta atgccgctgg agtaaacgat cacgccagca ggacgccgca tgcactgcaa
    18061 gggggcgtgt atgaaaccac tgctggatgt gttgatgatc ctcgatgcct tagaaaaaga
    18121 aggcagtttt gcggcggcgt cggccaaact ctacaagacg ccgtccgctc tgagttacac
    18181 cgttcacaag ctggaaagcg accttaatat tcaattgctt gatcgtagcg gacaccgcgc
    18241 taaattcacc cgcaccggaa aaatgttatt agagaaaggg cgggaagttc tgcataccgt
    18301 gcgggaactg gaaaagcagg cgatcaaact gcatgaaggc tgggaaaacg agctggtgat
    18361 tggcgtggac gacacgtttc ctttttctct gcttgcgcca ctgattgaag ctttttatca
    18421 acatcatagc gtgacgcgcc tgaagtttat caatggcgta ctcggtggtt catgggatgc
    18481 cctgactcag gggcgagcgg atattattgt tggcgcgatg catgagccac catcttccag
    18541 tgagtttggt ttttcacggt tgggcgatct ggaacaagtc tttgcggtcg cgcctcatca
    18601 tccgttagcc ctggaagaag agccgttaaa tcgtcgtatt attaagcgat atcgggcgat
    18661 tgtggtgggg gatactgcgc aggcaggcgc ttctacggcc tcacaacttc tcgacgaaca
    18721 agaagccatt accgttttcg attttaaaac caaactggag ctgcaaatta gcggcctcgg
    18781 ctgcggctat ttaccccgtt atctggcgca acgttttctc gatagtggcg cgttaatcga
    18841 gaagaaagtg gtcgcccaaa ctctctttga acccgtctgg attggctgga acgaacagac
    18901 cgcaggcctt gccagcggct ggtggcgaga tgaaatttta gcaaatagtg cgatcgccgg
    18961 tgtttatgca aaatctgatg acggaaaatc agccatttaa agaaaaatta ttatgacaag
    19021 cctctcattc tcttgtcatt tcccccccat ttaggcacaa tgcgccgctg tcaaaaaatg
    19081 actaaaaacc gacgtttcat cagcgtcggt tattttttgc ttcaaaccaa tcattcatac
    19141 caagaggccg ggcttcgtac cggatagata tttactaaaa atcgacagtt gttgtcgctg
    19201 aggaatccaa aaaaatgggg caattttttg cttacgcgac ggttatcacc gtaaaggaga
    19261 atgaccatgt cgcataacgt tactccaaac acctctcgcg tggaattgcg taaaacgctt
    19321 acgttagttc cggttgtaat gatgggtctt gcctatatgc agccgatgac gctgtttgat
    19381 acttttggta tcgtttcagg cctcacggat ggtcatgtgc cgacagccta tgcgttcgca
    19441 ttgattgcga tcctgtttac ggctctgagc tacgggaagc tggttcgccg ctatccttct
    19501 gctggctctg catacactta cgcccagaaa tccattagcc cgactgtcgg ctttatggtg
    19561 ggttggtctt ctctgctcga ctatctgttc gcgccgatga tcaacattct actggcgaaa
    19621 atctattttg aagctctggt gccttccatc ccatcgtgga tgtttgtggt ggcgctggtg
    19681 gcctttatga ccgcctttaa cctgcgtagt ctgaaatccg tagcgaactt caacactgta
    19741 atcgtcgtgt tgcaggtagt gctgatcgcg gtgattctgg gcatggttgt ttatggcgta
    19801 tttgaaggtg aaggcgctgg tacgctggcg agcactcgtc cattttggtc tggcgatgcg
    19861 catgttatcc cgatgattac cggggcgaca atcctgtgct tctcctttac cggctttgac
    19921 ggcatcagca acctgtcgga agaaaccaaa gatgcagagc gcgtgatccc gcgtgcgatt
    19981 ttcctgaccg cgctgatcgg cggcatgatc ttcatctttg caacttactt cctgcagctg
    20041 tacttcccgg atatctctcg ctttaaagat ccggatgcgt cacagcctga aatcatgctg
    20101 tacgttgcgg gtaaagcgtt ccaggttggc gcgctgatct tctccaccat taccgtactg
    20161 gcgtccggta tggcggcgca tgcaggcgta gcgcgtctga tgtacgtaat gggtcgtgac
    20221 ggcgtattcc cgaaaagctt cttcggttat gtacacccga aatggcgtac tccggcgatg
    20281 aacatcatcc tggttggcgc gattgccctg ctggcaatca acttcgacct ggtaatggca
    20341 acggcgctga ttaactttgg tgcgctggtg gcgttcacct tcgttaacct gtcggtcatc
    20401 tcgcagttct ggatccgtga gaagcgtaat aagacgctga aagatcactt ccagtatctg
    20461 ttcctgccga tgtgtggtgc gctgaccgtt ggtgcgctgt gggttaacct ggaagaaagc
    20521 tcaatggttc tgggtctgat ctgggctgct atcggtctga tttacctggc ttgcgtcacc
    20581 aagagcttcc gtaacccggt tccgcagtac gaagacgtag cataatcatc ttttctaata
    20641 atcaaccgga gtcgaatgaa tatttggctc cggttttttt attctttttt atcgaaatcc
    20701 tcattaagaa ccatttgttc taatttagag aatttggtta ttggtctgca ttccaccagt
    20761 catgaataat ttcctcatta acattcatct ggttaattaa tttatgtttt caatgatatt
    20821 aagcgggcta atttgtggtg ctctgctggg atttgtcatg cagcgtgggc gtttttgcct
    20881 gactggtggt tttcgcgata tgtatatcgc gaaaaataat cgcatgtttt acgccttgct
    20941 gattgcaatt tcggtacaaa gtgtgggggt ttttgcgtta attcaggcgg gcctactgac
    21001 ttacgaagcc ggggcgttcc cgtggcttgg tactgttata ggtgggtata tcttcgggct
    21061 gggaattgtt ctggcgggtg gatgtgccac cgggacctgg tatcgcgcgg gtgagggatt
    21121 gatcggcagt tggatcgcgc ttttctctta tatggtgatg agtgcggtga tgcgttctcc
    21181 acatgccagt ggtttaaatc aaaccttgca gcactacagt actgaacata actctattgc
    21241 tgagactttt aatttgtctg tgtggccgtt ggttgccgtt ttgctggtga taacgctctg
    21301 ggtggtgatg aaagagttga agaagccaaa actcaaagtc gcgaccttac cgccgcgccg
    21361 aaccgggatc gctcatattc tgtttgaaaa acgctggcat ccctttgtca ctgctgtact
    21421 catcggtttg attgcgcttt tagcctggcc cctgagtgaa gcaaccggac gcatgtttgg
    21481 gttaggaatc acttccccaa cggccaatat tctgcaattt ctggtcgcgg gtgacgtgaa
    21541 atacattaac tggggcgttt tcctggtgtt agggatcttc gtggggtcat ttattgcagc
    21601 caaagcgagc cgtgagttcc gcgttcgcgc agctgatgca caaacaacat tacgtagcgg
    21661 gttaggtggt gtactgatgg gcttcggtgc cagtattgca ggtggttgct ctatcggtaa
    21721 tggactggtt atgactgcaa tgatgacctg gcagggctgg attggccttg tatttatgat
    21781 tctcggagtc tggactgcgt cctggcttgt gtatgttcga ccgcagcgta aggcgcgact
    21841 ggcgactgct gcggcaaatt aagtaaggtg taaagatggc gattaaaaag ctggatgtgg
    21901 tgacgcaggt ctgtccgttt ccactcatag aagcaaaagc agcactggca gaaatggcca
    21961 gcggcgatga gttggtaatt gagtttgatt gcacccaggc aactgaagca atcccacagt
    22021 gggcggcaga agaaggacat gccatcaccg attatcagca gattggcgat gcagcgtgga
    22081 gcatcaccgt tcaaaaagcc tgactcaaca ttgtcctccg ccgtaccagc ggcggaggct
    22141 tcaaattaga cgatctcttc cgcgtactgc caaagcgctt ttaacagcgc cactttctct
    22201 ttgtcatcgg catattgttg taccagcatc tgcaattcat cagcataacc ctgcaaaaac
    22261 tctggcgtga atacctggcg gcggtgctcc agccagcgtt gctgctcggc ataatccagc
    22321 gtccccggga agttgcgtgc ccgataattg aacaacagct tttcaatccg tttatcaaca
    22381 aaagtgatat ccagtgccgg taaattacgt ggctcggttt ccagcacaat tttcattgct
    22441 gcacgatctg cgtcactgaa aaagccgtta taaagctgtg catccacgtt atctgtaggc
    22501 gtaaacggtt cggcttccgc gaatatcgcc accacttttt cacgcacttg cggattttca
    22561 cgcagaattt tcaggttatc gaggcaatgc tgacgattaa ttcccagtcg gtcggcatct
    22621 tccgggcgta gcgtattcgc ctgggccagc accggacatt tattgatatg caccagctta
    22681 accggaacgg cggcgttatc gccaagatcg gtttttgcgg tatataaacg ctcgcgcaat
    22741 gtgtcgctat ccagttccag taatggcgaa atgtctcctg ccaaatccac cataattacg
    22801 gcattgtgat tttcaggatg ccacgccagc ggtgccaccc agctggtatt gccgcgccat
    22861 gctccaaaca ttccggaaac gtgcaccagg ggtttcatct gcggaacatc aatcaacgcc
    22921 atcagtttgt gtttattacg atgggtaaag agataatcaa acaggcgtgg ctgacgcgtt
    22981 tttaccagct ttgccatcgc aatagtggcg tacacatcag ccatcgcatc gtgggcgttg
    23041 ctatgttcaa taccattcgc tttggttaaa tgctcaaggc gaaagctcgg tagaccgtca
    23101 tcattttcag gccagtttat tccttccggg cgcagggcat aacaggcacg cataacatcc
    23161 agtaaatccc agcgcgagtt atcatgctgc cagctccagg cgtaaggatc gtagaaatta
    23221 cgataaaaaa tgttgcgtgt gacttcgtcg tcgaaacgca cattgttgta gcccagaata
    23281 caggtcttcg gtacggtaaa aagcgagtga atacgggcgg caaacgcggc ttcgttttct
    23341 cctttcgccc gtgcttcctg cgggttaata ccggtaatta atacggctcc tggctggggt
    23401 aaatagtcat cagcgggctt gcagtaaaag acttcgggtt cgccgatgac attgaattcg
    23461 ctatcggtgc gaatggctgc gaactgtgca gggcgatcta acgcggggtg cgtgccaaag
    23521 gtttcgtaat cgtgaaagag gaaggtaggt tgtttatctg tgtctgtcat tgtctagcat
    23581 tgtccactgt agttaattaa ctgtctgtta taactcgaaa aacaaccatt ttttcacctg
    23641 agtatgtcta cgaacgtcaa atatagttgt ttactgtgcg ctgttgtcca tgtgatattg
    23701 tgtaccagat gtgtaccaaa ccattattcc tgagcgtacc aaatattatt tatggctata
    23761 agcgacacaa aactgcgtag tatttatggt aaaccatatt cgggcccaca agaagtggct
    23821 gatgccgatg gcctcagcgt acgaatttca ccgaaggggg tcatccagtt ccagtaccgc
    23881 tatcgctggc atggcaagcc taatcgactt gggcttggtc gatacccatc cctgtctttg
    23941 aaggatgcca gacagatcac tgctgacttg cgaaagctct atttctcagg aacggatcca
    24001 cgcacttatt ttgaagagaa ggtggagaac tccatgacgg tcgcccagtg tctcgactac
    24061 tggttcgaca actacgtctc tacaactctc agagaaaaga cccaggcact ttaccgatca
    24121 acggttatga agcgcatgca tgacgccttc cctaatcgtc cggcatcttc tatcacggtt
    24181 aagcaatggg ttgacctgct taccgaagaa gaaaaagata atccacgccg agcaaggcag
    24241 gtgctaagtc aactaagatc agcaattagt tggtgcatgc ggcgtcagtt gatagatagt
    24301 tgcgcaatta tgagcatcca gccaagggac ttcggctccc gcgctgaggt aggggatcgg
    24361 gtactgtcgt atcacgaact ggctaagatt tggcttgcta ttgaaagaag ccgtgcgtct
    24421 acgtcaaata agctccttca tcagatgctt atgctgtggg gggcgaggct ctcagagctt
    24481 aggctggcaa caaagacaga atttgacctg ctggacaacg tatggaccgt accgaaagag
    24541 catagcaaga tgggtaatgt tatccgccgt ccaatcttcg aacaaattaa gccttttctc
    24601 gaaaaggcca tgacaacgta caatgatgtt cttttccctg gagaagacat aaacaaaccg
    24661 atcagcatcg ctgcagccaa ccgattcgta aatagaataa ggggagggat ggatctaggt
    24721 tactggcgaa cacatgattt cagaagaacg cttgttacac gcctgtccga gatgaatgtc
    24781 gagcctcatg ttactgagcg aatgctcggt catgaacttg gcgggataat gtccgtatac
    24841 aataaacacg actggataga ggctcagcgc aaagcgtatg agcttcacgc tgataaattg
    24901 ttctggcaca tcaggagcat ttctgattaa cgccaccgtt aagaatccac ccttcaacag
    24961 cttcacgaag gtatgatttg gggtgggttc tgactggctt cggaaatccg tgccgtttgg
    25021 tatagttcca gattgtctga cgtgatgaaa caccgagctt gttcatcact tctttctcag
    25081 gaatcaggct ggtatcggtc atcttaattc tccaggcaaa aataaaccgc catatagcgg
    25141 ctctatcaga tatgaacagg cctcatcgag tgtgaggcgg gttagtcctt gcgtagctcg
    25201 ctgattcttc tgtaagtctc tggtgctttg ttcccgtacg tcttcatttc agacttcaac
    25261 agagcaacga gtgaatccca ttcgttgagg attcctttga atgccggaac gcgctttgca
    25321 accttgtcga atgaatctct gatttctgga atctgatcaa caagtgcaac gcatcgtctg
    25381 aaatcggata cgtcatgtgg agcgccgaag tgatgaccat agatattctt tttcagtcca
    25441 catgcgattg aggcaagagt tgcgctactg atgccgacat cgccagtcga ttgccatttc
    25501 aaaaccttca tagccaaatc tgacatttct tgtctccaat aaaaaaccgc catcatgcgg
    25561 tttggtgttc tttcagttct tcaatttgaa tattggttac ttctgcatgt gctatctgcg
    25621 cccatatcat ccagtggtta tagcagtcat tgatgtcctc tgcttcgata actctgtcga
    25681 atggctctcc attccattca cctgtgactc ggaagtgcat ttatcatctc cataaaacaa
    25741 aactcgccgt agcgagttca gataaaagaa atccccgcga gtgcgaggat tgttattcat
    25801 tgctgatatt cacctttatc gcgtatacct ttaccggttt atcgccgaag tgcggatgtg
    25861 tgattgtctt gatttcatat ccgtcacacg gaacatcaat tctgcggctg gaatcgtcgc
    25921 gcttcggata tccctttgtg ataatcaggc ggtcatattc ccggagcata attcgcttat
    25981 tccagtagtc attacacagg cgatactctt ctgttttctc tccgcgaatc atggcatcga
    26041 agtattcacc tttaacggca agttgcaggt tagccacggt taaccttctg cggcggttct
    26101 ggtagcggca tccagtgtga tggtatccac gacgcaccag gaattatcca cccatcatta
    26161 gcgtcaggat gacccgggat gtaagtcgcc catttcattc gccagtcacc tttcctgtca
    26221 aactccacgg caacaagaac ggctgttttg gtatccggca ttcgctcact acagcttatc
    26281 caactatccg gagttgccgg agagttgcca tttacatcga agtttggctc tgcgtcctga
    26341 accaggagga tgtaaccatt cttggctgta tcaagttcta acgcctcggt gacggtaccg
    26401 aaatagcgat tacctaaatc cgcatcacaa gtgcttacat caatggaaac ctccatgcct
    26461 tcgattaatt ctggcaagtt gtaagtttgg cttacaggtt ctgctcccag tgatgccagt
    26521 gcaatccgtg ccagttccat ttgttcgcca cgggtaagtc cgttttcaag cggattttta
    26581 atgaacaatt caatacgttc tttggtaata gtggtcattt gttaatcctc aaaactttat
    26641 gcccgggcgc aaaagcacgc gttttgtctt tgcttattcg ccagccatcc ttgcgcgcct
    26701 cttttgcaca gccagcccat gacgtaccta tatactcacc gaagtctggc gacttatatt
    26761 tgccatctgt acactggagg caatcacaat agagatgcat ggtgtaactt gcagcgatag
    26821 ccatatcact ctcctttagt gcgcaagtgg tttttccagc ggttttgcgc agcgctgggc
    26881 tttttgcaac aactgtgccc catcaccccg caacaccccg tcaacctcac tcgtctgtta
    26941 ctaatcctca accatcgcca gaccccaaca ccgtttctgc gagctaacag aatctttgct
    27001 ttacggtttt tcatcgtttt gctctcctgc gtctctttgc tgctcgtcgt gccgctgcaa
    27061 taccggtatg gcggcgcttt ggtgccggga tgatgttgtc agccatcagg acatgtggct
    27121 ttgcaattag cgcagaagcc caaaatcgag tcggatacgg taacaagccg atacatgcca
    27181 cacgcattac tcacctcctt tgatgcgaat gccagcggcg cgctcggctt cactttgttc
    27241 ccaaaaccac ttgtgaagcg ccataagctt ttcgtcaatc ggtgcatatt tgcgattaaa
    27301 gtaggcctga gcatctttct cagattcgtc cggcaattcg cctgggccaa acagtgtgtt
    27361 ataaatccat gctagtccgc tcttagcgtc gccagttgcc tgccattcga taatggcagc
    27421 ctgcatgacc agaatgtttt tcccgattaa taggtccagt tctttgtacc ggttgcggat
    27481 gtatgcattc tcgctttgta attttgcgtt gcgcttttct gaggcttcaa gtaacgcctg
    27541 cttatcgcgt agagcttctt ccagttcagc aacatggcat tcactatcaa taaggttgtt
    27601 ctctgctgct tcaagctcaa cacgcagctt cccaaccgta agcgcaatct cctcgttctc
    27661 ctggtcgcgg cgtttgatgt attgctggtt cctttcccgt tcatccagta gtgccagcac
    27721 ggtagccgga ttagccttgg caacaaaatc ccggactggc ttacaatcaa tctccgcaat
    27781 gggttgatac gatgtgtagc catgctgtct tgtataacta ccgtgacgaa taacgaaaaa
    27841 atcaccattt atttttttag cctgccactt atcttcaccg gctttctctg ccgcttcacg
    27901 cagtgcctga tagtcaatct tgttcatgtc acatcaccct gaatccgttg catttacgta
    27961 agaaatcgca gatatagccc ttcatttttt catgccaatc tcgatcattc ccattgcacc
    28021 aaccatcagg tggagtccag ttttctatca gatcagccat tttctttgct ttcgccggag
    28081 tagctgttgc ggtatcgcag taatgacgag tgtcaaccaa cgaatccata ccatcgatat
    28141 caagtacgca aaaccatgtg tgattcggaa ttcctacagg tggtatttgt tgcccacgtc
    28201 gacgtttatc aataagatat acactcactg gttgcctcct ttgcgaagct cagcggcgaa
    28261 ggctactgcg tgatcatgat gttcaagtgt gtatgcacac tccgcaaaca tctccacgcc
    28321 ctgcgcccgt acttcagcca gaaaagcatc ggtggctggg gttttgattt cgttaagcgc
    28381 atcactgaat ccaccacgct ccatacctag ctctgcttcg taatcggcat cgaatgcagc
    28441 gtctttgcag aacttcttca tccccgcact ctccgctgcc agcgccacgc acttggcctc
    28501 aaggttatca atcgtgattc cagcagaacg acactcccgc aacgccgttt ccagttttga
    28561 ttcaagttca ccgaacttac gcaccagat
//
LOCUS       DAZKHC020000057        28558 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZKHC020000057 DAZKHC020000000
VERSION     DAZKHC020000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.057"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..242)
                     /locus_tag="ACHOSM_RS14245"
                     /old_locus_tag="pgaptmp_002849"
     CDS             complement(<1..242)
                     /locus_tag="ACHOSM_RS14245"
                     /old_locus_tag="pgaptmp_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGU4559333.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHOSM_RS14250"
                     /old_locus_tag="pgaptmp_002850"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHOSM_RS14250"
                     /old_locus_tag="pgaptmp_002850"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HGU4559334.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="ACHOSM_RS14255"
                     /old_locus_tag="pgaptmp_002851"
     CDS             2255..3904
                     /locus_tag="ACHOSM_RS14255"
                     /old_locus_tag="pgaptmp_002851"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGU4559335.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3955..4557)
                     /locus_tag="ACHOSM_RS14260"
                     /old_locus_tag="pgaptmp_002852"
     CDS             complement(3955..4557)
                     /locus_tag="ACHOSM_RS14260"
                     /old_locus_tag="pgaptmp_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGU4559336.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            5077..5976
                     /locus_tag="ACHOSM_RS14265"
                     /old_locus_tag="pgaptmp_002853"
     CDS             5077..5976
                     /locus_tag="ACHOSM_RS14265"
                     /old_locus_tag="pgaptmp_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4559337.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLTAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     QELSRRVNLFMQWLAGVMKEYLD"
     gene            6025..7038
                     /gene="yhjD"
                     /locus_tag="ACHOSM_RS14270"
                     /old_locus_tag="pgaptmp_002854"
     CDS             6025..7038
                     /gene="yhjD"
                     /locus_tag="ACHOSM_RS14270"
                     /old_locus_tag="pgaptmp_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HGU4559338.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7449..8771
                     /locus_tag="ACHOSM_RS14275"
                     /old_locus_tag="pgaptmp_002855"
     CDS             7449..8771
                     /locus_tag="ACHOSM_RS14275"
                     /old_locus_tag="pgaptmp_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4559339.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(9005..11065)
                     /locus_tag="ACHOSM_RS14280"
                     /old_locus_tag="pgaptmp_002856"
     CDS             complement(9005..11065)
                     /locus_tag="ACHOSM_RS14280"
                     /old_locus_tag="pgaptmp_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU4559340.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIDIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQVDFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(11135..11902)
                     /gene="pdeH"
                     /locus_tag="ACHOSM_RS14285"
                     /old_locus_tag="pgaptmp_002857"
     CDS             complement(11135..11902)
                     /gene="pdeH"
                     /locus_tag="ACHOSM_RS14285"
                     /old_locus_tag="pgaptmp_002857"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HGU4559341.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            12134..13063
                     /gene="kdgK"
                     /locus_tag="ACHOSM_RS14290"
                     /old_locus_tag="pgaptmp_002858"
     CDS             12134..13063
                     /gene="kdgK"
                     /locus_tag="ACHOSM_RS14290"
                     /old_locus_tag="pgaptmp_002858"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HGU4559342.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     HAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(13159..14655)
                     /locus_tag="ACHOSM_RS14295"
                     /old_locus_tag="pgaptmp_002859"
     CDS             complement(13159..14655)
                     /locus_tag="ACHOSM_RS14295"
                     /old_locus_tag="pgaptmp_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU4559343.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14876..16162)
                     /gene="dctA"
                     /locus_tag="ACHOSM_RS14300"
                     /old_locus_tag="pgaptmp_002860"
     CDS             complement(14876..16162)
                     /gene="dctA"
                     /locus_tag="ACHOSM_RS14300"
                     /old_locus_tag="pgaptmp_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HGU4559344.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(16345..18333)
                     /gene="hmsP"
                     /locus_tag="ACHOSM_RS14305"
                     /old_locus_tag="pgaptmp_002861"
     CDS             complement(16345..18333)
                     /gene="hmsP"
                     /locus_tag="ACHOSM_RS14305"
                     /old_locus_tag="pgaptmp_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HGU4559345.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYQEEK"
     gene            complement(18415..21888)
                     /gene="bcsC"
                     /locus_tag="ACHOSM_RS14310"
                     /old_locus_tag="pgaptmp_002862"
     CDS             complement(18415..21888)
                     /gene="bcsC"
                     /locus_tag="ACHOSM_RS14310"
                     /old_locus_tag="pgaptmp_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HGU4559346.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQTTTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLENIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSQSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21870..22976)
                     /gene="bcsZ"
                     /locus_tag="ACHOSM_RS14315"
                     /old_locus_tag="pgaptmp_002863"
     CDS             complement(21870..22976)
                     /gene="bcsZ"
                     /locus_tag="ACHOSM_RS14315"
                     /old_locus_tag="pgaptmp_002863"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HGU4559347.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22983..25322)
                     /gene="bcsB"
                     /locus_tag="ACHOSM_RS14320"
                     /old_locus_tag="pgaptmp_002864"
     CDS             complement(22983..25322)
                     /gene="bcsB"
                     /locus_tag="ACHOSM_RS14320"
                     /old_locus_tag="pgaptmp_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HGU4559348.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYEL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(25333..27854)
                     /gene="bcsA"
                     /locus_tag="ACHOSM_RS14325"
                     /old_locus_tag="pgaptmp_002865"
                     /pseudo
     CDS             complement(25333..27854)
                     /gene="bcsA"
                     /locus_tag="ACHOSM_RS14325"
                     /old_locus_tag="pgaptmp_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            complement(27851..>28558)
                     /gene="bcsQ"
                     /locus_tag="ACHOSM_RS14330"
                     /old_locus_tag="pgaptmp_002866"
     CDS             complement(27851..>28558)
                     /gene="bcsQ"
                     /locus_tag="ACHOSM_RS14330"
                     /old_locus_tag="pgaptmp_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HGU4559349.1"
                     /translation="TTITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWA
                     RAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQIKASGR
                     YQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRI
                     GSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLAN
                     WCLLNYSGLKTPVGSAS"
BASE COUNT         6730 a         7879 c         7195 g         6754 t
ORIGIN      
        1 ttaatggata aatccatcag tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatcgc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtcacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccgcc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 taaacttcga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaggatt tcgacgccat ttcaatcggg ttcaacggcg gtccacctgc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gtagcgcggt ataacgcgtt ggtggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaagcctaa aaccttatta ttttcactga cagcagatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttatcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc cccgacagta aaacctttcc cgactgcgca cccaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccgg gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgctcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggta tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt gcgcagtcgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acagtggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgaagc tggctgaaga cggtgaatca gttctatctg gaacagcaca aactgatcga
     3781 aaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     3961 ggagatattt aacatcattg ccgcctgggt gcgatttttc acttccagac ggcgatacag
     4021 ggattccaga tgcgctttta ccgttccggt actgatattc agcgctctgc cgatctcctt
     4081 atttgattcg cccgccgcta acatggttaa aatctcccgc tggcgggcgc ttaacgattt
     4141 gagatcttta atgtcctttt ccggcgtcgt ccgccagtct ccaggcagaa acatcatccc
     4201 catcgccgca ctatttaccg ccaacgcaaa tgtctcgacg gttgaatcac gaggcacaat
     4261 ggccagcaca ttaaaatgga taacttcctg taaccaccgt ttattgcaat ccgtcgccgt
     4321 gattaacacc ttaacctcag gaaactgcac cacggttttt tgcagcaacc agtagcaaaa
     4381 ctcaccatcc tgatcgccat cgagcataac taaggcttca gggtaacttt ccagcttttg
     4441 ccataactcg tctgcctgac tggccccctg aatactcact cctggaatac gctgctgtaa
     4501 actgattttc attccatgaa taaatattga ctgcctgtca aacatgacta tttgcataac
     4561 tgaatctcca cctgaatacg ttaaaaagac ttaagtagtg gaagggtatt acccgcgaga
     4621 aaaaataaga attcgccatt tggcggtggc cattctacag agatgacgcg tagaaaatag
     4681 ttaccgatat aaatagttac agctaaacgc ctgaaattac atgtcgaggg cactatttaa
     4741 aacaattttg aggatttcct tatattggtg gttagtacgc atgcaattaa aaatgaaact
     4801 ccgcgaccac aagcaaaaat aacaaacggc aatgagacaa aaataagcac aaatagccaa
     4861 cacgtcctct attcacttta aagggaatcg ctgaaaaata cgctctgttt agggggattc
     4921 acctttctca gaaagctatt ccgccctttt cctgctgaga aatcgccaca ttcgtcatga
     4981 caacattgtg aaacccggca ttagatgtta gaaaaaacaa taaacaatgc gatatgcgcg
     5041 ttactgtttc gctgtgaaca acaatcggtc aaagaaatgg ataaaattca cgcaatgcag
     5101 ttgttcatca aagtcgcgga gctggaaagt ttttcccgcg cggcggattt ctttgctttg
     5161 ccaaaaggaa gtgtttcgcg ccagatacag gcactggaac atcaacttgg cacccagctt
     5221 ctccagcgca ccacgcgacg ggtcaaactc acgccagaag gcatgaccta ttatcaacga
     5281 gcaaaagatg tgttgagtaa tctcagcgaa ctggacggtc tgtttcaaca ggatgccacc
     5341 agtatcagcg gtaaattacg catcgacatc ccgtcaggaa tcgcgaaaaa cctgttactg
     5401 ccgcgtctgt cggaatttct ctatcagcat ccgggaattg agctggaact gagtagccat
     5461 aaccgtccgg tagatattct tcatgatggt tttgattgcg tgatacgcac tggcgcgtta
     5521 ccggaagatg gcgttatcgc ccgtcccctc ggcaaactaa ccatggtcaa ctgtgccagt
     5581 ccgcactatc tgacgcgctt tggttatccg caaagccccg atgatctgac ttcacacgca
     5641 atagtgcgtt acacaccgca cctgggtgta catccgttag gttttgaggt tgccagcgtt
     5701 aatggcgtcc agtggtttaa gtctggcggc atgttgacgg taaacagtag cgaaaactat
     5761 ctcaccgccg gtcttgccgg tctggggatt attcagatcc cgcgcattgc cgtgcgcgaa
     5821 gccctgcgtg ccgggcggct tattgaagtg ttgccaggct accgtgccga gccgctctcc
     5881 ctttcgctgg tttatccgca gcgtcaggag ctttcccggc gtgtaaacct gtttatgcag
     5941 tggctggctg gcgtaatgaa agagtacctg gactgaccga ctatactttt taagaatgac
     6001 cacgacagaa ggacaaaaga gcggatgacg caggaaaacg agatcaaacg tcccacccag
     6061 gatctggagc acgagccgat taagcagttg gataatagcg agaaaggcgg caaagttagc
     6121 caggcgctgg aaaccgtcac caccactgcc gaaaaagtcc aacgccaacc ggttattgcg
     6181 cacctgattc gtgcgacaga acgctttaac gatcggctgg gtaaccagtt tggtgcggct
     6241 atcacctatt tctcattttt gtcgatgatc ccgattttga tggtgtcgtt tgccgcaggg
     6301 ggctttgtgc tggcctccca tccgatgttg ctacaggata tcttcgacaa aattctgcaa
     6361 aacatcagcg atccgacgct agccgccacg ttgaaaaaca ccatcaacac cgccgttcag
     6421 cagcgtacga ctgtagggct tgtcggcctg gcggtggcac tttattccgg catcaactgg
     6481 atgggtaacc tgcgtgaagc gattcgtgcc cagtcgcgcg atgtctggga acgctcgccg
     6541 caagatcagg agaagttctg ggtaaaatat ctgcgtgatt ttatttcgct gattggtttg
     6601 ttgattgcgc tgattgtaac gctttcgatc acctcggttg ccggttcggc gcagcaaatg
     6661 attattagcg ccctgcacct gaacagcatt gagtggctga aaccgacgtg gcggttgatt
     6721 ggcctggcga tttccatctt cgccaactat ctgcttttct tctggatctt ctggcggtta
     6781 ccgcgccacc gtccgcgcaa aaaagccctg attcgcggaa catttctggc tgctattggt
     6841 tttgaagtga ttaaaatcgt gatgacctac accctgccat cgctgatgaa atccccctct
     6901 ggcgcagcat ttggttccgt gctggggctg atggcatttt tctacttctt cgcccgtttg
     6961 acgctgtttt gcgcggcgtg gattgccacc gccgaatata aagacgaccc gcgaatgccg
     7021 gggaaaacgc agccttaaaa taggccggat gcggcgtaaa cgccttatcc ggcatacgat
     7081 tttattcggc ctggctcccc gtaggccgga taagatgcgc cagcatcgca tccggctata
     7141 atgcgcacat aacctcttga aactcgtccc cagagcctct tcagccatct atttgggagc
     7201 aaacaatttc attccaactc ataaccccag catataaatc cagttggtaa cttttattta
     7261 acctgaaacc agttttatcc actatttata aaattatgtg aagcatttca tagaagaaaa
     7321 atcactggcc taaacattat cccctttttg cctggttttt gaccatttcc gcgatttgtt
     7381 acacattgaa atatcacttt tgctgtgcgt aatatggcta ttcgttagcc aaaaaataag
     7441 aaaagattat gcaagcaaca gccacaacac tcgaccacga gcaagaatac acgccgatca
     7501 actcgcgtaa taaagtcctt gtcgcctctc tcattggcac agccattgag ttcttcgact
     7561 tttacattta cgccactgcg gccgttattg tgtttccgca tatcttcttc ccgcagggcg
     7621 atcctacggc agcaacgcta cagtcgctcg ccaccttcgc catcgccttc gtcgcgcgcc
     7681 ccattggctc tgccgttttt ggtcattttg gcgatcgcgt tgggcgtaaa gcgacgctgg
     7741 tcgcctcgtt gctaacgatg gggatttcga ccgtggtgat tggtctgctg ccaggctatg
     7801 ccacgattgg tattttcgcc ccgctgctgc tggcgctggc tcgatttggt cagggtctgg
     7861 gcttaggtgg tgaatggggc ggcgcggcgc tgctggcgac tgaaaacgcc ccaccgcgca
     7921 aacgtgcatt gtacggctcc ttcccgcagc tgggcgcacc gattggcttc ttctttgcca
     7981 acggcacttt cttgctgctt tcctggctac tgaccgacga gcagtttatg agctggggct
     8041 ggcgtgtgcc atttatcttc tcggcggtgc tggtcattat cggcctgtat gttcgcgtgt
     8101 cgctgcatga gtcgccggtg tttgagaaag tcgctaaagc gaaaaaacag gtgaagatcc
     8161 cgctgggtac gctgctgacc aaacatgttc gcgtaaccgt actgggtacg ttcattatgc
     8221 tggcaaccta tacgctgttt tacatcatga cggtctactc tatgaccttt agtaccgccg
     8281 ccgcgccagt tgggcttggc ctgccgcgta acgaagtgtt gtggatgttg atgatggcag
     8341 ttattggttt tggcgtgatg gtgccagtcg ctggattact ggctgatgcc tttggtcgcc
     8401 gtaaaagcat ggtaatcatc accacgctga tcatcctgtt cgcgctgttc gcctttaacc
     8461 cactgctcgg ttctggcaac ccgattctgg tttttgcctt cctgctgctg gggttaagtc
     8521 tgatgggtct gaccttcggg ccaatgggtg cgctgttacc agagctgttt ccgacagaag
     8581 tgcgttacac cggagcatcg ttctcttaca acgtagcgtc gattctcggg gcttccgttg
     8641 cgccatatat cgcagcctgg ttgcagacta actacgggct aggtgcggtg gggttatatc
     8701 tggcggcgat ggctggcttg acgttaatcg ccctgctgct gacccatgag acgcgacatc
     8761 agtcgttgta agtatcgtgt gggcagccac cctcacccca gccctctccc tgaaagggag
     8821 agggagaaaa gcggagcgag atgttatatc gcgaacataa cagccctccc ccagaaagtg
     8881 aacggaaaag cggtactcgt tgtttcaaca cgcacaaaac agtcccctcg cccctttggg
     8941 gagagggtta gggtgagggg aacacgctgc tcccgaactc accagcaaac acaatccaac
     9001 ctcatcactt cttcatctgc gacaatatcg tccgacactg attagcctcc ccttcggaag
     9061 gtgagatcag tgccagtaac gccgccgccg gtgttaccag cgttgccagt gccgcagcaa
     9121 cagcaccacg ggcaatcagc ggtccggctt tcaccccagc ctgcggattt ttaaacgtcc
     9181 cacgcacata cagcggcgaa cgcagtgtga taatccgaat ccctttactc tccggatcaa
     9241 tagtcaaatc cagctgttcc gaagcaaaac ttgccgtgcc ggtaacatta atcaacgcgt
     9301 tctcagtatc gaaagcaaaa atctgcgggc gcgccacgcc gttggcaata ttcagattcg
     9361 ccgccgcgca gttcacccgc acctcatcgt caccaaatat cgcaccgaca atgtagttgc
     9421 cgacattcag cccaacaatc tccatcaggt tgcggctcac cagcccgtca ttcatcaaca
     9481 gtttcaggtt gccgttactg ttgcctaaaa gcgccgccac cgagttaccg ctaccgcgta
     9541 gttccgcgtc accgttcatt tcccccagcg tcttctgcat cagttccaca tcgggcatca
     9601 gttctttcag tttcagtcga cgagcctgaa tatctgcccg cccctgcatc ggctttttat
     9661 cgccttccag atggatattc gccgcaatgc tgccgcccgc catgccaaat ttcagcggtt
     9721 gcaggcgcag gtcagcattt ttgaggatga tatgagtaga aagatcgcta atcggcaggc
     9781 tactgccatg ctcaatgcgc cgccctttga agcgaacatc ggcatccata acgtcccatt
     9841 tatcggtttc gaagcggtca taaggcagca ctttgcccgc aggctgaacg cttttttcgc
     9901 ccttcttctg ttcagaccgt ttcgactttt ctgccccttt cccggaatca acgccaatca
     9961 acggtcccag gtccgccagc cgcaattgcc gcgactcgac atcaccttcc agttttggtc
    10021 gtggctttcc ggtggtgtag accagagaac cgtggatatc gctatcgcca attcgcccat
    10081 taaaaccgcg ataatcaaag accgacgatt tttcagtgtc gattttcgct accagccgac
    10141 catccgtttc aaacggcggg gtatcgggca gcagaacgcc cgtcagttca tagagatcac
    10201 ccagtgaatc gccagaaaat ttaagccgta aatcgacacc gcccatcttc attgggtcat
    10261 tcacgacgcc atcaaaagca acacgggtgt taccagagcg gaaatcaacc tgtaccggaa
    10321 acggcgtccc ttcgccacgc agcgccagca taccgcctat ttttcccgta cccgtgagcg
    10381 gttcaccgtt ataacgtccc tgcgccttca ggccaaaaac gtaatcgccc accttttctt
    10441 tatccgcttt acctttcgat ccagtaactt cgctgaacgg cagcggcttg cctaacggat
    10501 caacaaaaat ctccagatcc gctttgctta ctttgtcatc aatgtcgatc cgcccttgat
    10561 cgaaaagaat attatccagc cgaaacgacc atgccgacgg ctttgcattc gcgtctttgt
    10621 tgtcatcgtt ggcaagatta aacgtccagt tattgttctt ttcagagagg cgaatcaggc
    10681 gcgcgtcggg cttttcgagc ttgatccacg gcagccagac cgttttggtc agcagcgcca
    10741 gcggtgccag cgttgcctct actcgcggca aatgcaccat cgtgacttcg ggaatatccg
    10801 gtggattacc aagaatgatg tcttctgcat gtacatgagg ccacggcacc cagctgcgcc
    10861 agccagtttc ttgtttttgc cgctcccaca ccacgcccag atcgccacgg atagcgaacg
    10921 gacgattcaa ctccgcagag actttctggt tgatggtcgg tttgagtcga ttccagtcaa
    10981 aagttgcaat caaaatgatc gccacgacaa tcaacaacaa gaaagcccct gaaatcgcag
    11041 cggttatttt gcctgccttg ctcatcgttc gcttcctcat attcttcctg tgccagtcct
    11101 aaagatagtc cagccaggcg gaaaatgagg cagcttatag cgccagaacc gccgtattca
    11161 gcgtttctat cggtgccggg cgtgaaagaa accagccttg tgcggcgaat gcgggcgagt
    11221 tctgtacatc acgccactct tccggcgttt ctacgccctc aacaatcacc ccgcgacaat
    11281 agcgattcat tagatgtaaa agctgagaaa agagtgtgcg tccttccggc gactgacgca
    11341 gcatcacaaa cagttctcgc gcgattttga tgtagtcata acgcacttca cttagtgcag
    11401 agaaatttgc catcccggta ccaaaatcat ccagccacag cgggccaaat tcacacatcg
    11461 aggcaaaggt tgaatctttc ggcagacgga tatgctccac cagttcgaaa cgcagccagg
    11521 gaagacgctc aatttggcgc aggatttttg gttgctgacg cagggcgatg agcgtagggc
    11581 catcaatatt gaccgatgcc agcaggccgt gctctataaa gaagtcggct ttttgcgcca
    11641 gcaaatcaat ctgctctttc acaacctcca tccgatggct gacggtgatt tcagtaaaat
    11701 agcgatccgg cggcaggcgt tgcgaagggt tcaagggatg cgtgaccacc gttaatagct
    11761 ccacggccat taaccgcccg catgtttgat agatcggctg ccaggtgtaa gcacgctcac
    11821 actgcaacca aaaacgccgt tcctgcaagc tctcgatgct tgcttcaggg ttgcttattc
    11881 gctggataac ctgccttatc atcaaagatg tcctgtgttt aaaagtgatg ctgcccggac
    11941 tcgacaaaga ttatcggcgc gtaagggcag aactttaccg cctggccggg gtaaatttcc
    12001 tcactccaca acggaatctt tcaggaatac gttctggctc aaaaaaataa tggaacactg
    12061 ttttaatata gttgaccagc aaaccaccac agcgcaaact aacgctaatt ttttacagat
    12121 caggttcacg actatgtcca aaaagattgc cgtgattggc gaatgcatga ttgagctttc
    12181 cgagaaaggc gcggacgtta agcgcggttt cggcggcgat accctgaaca cttccgtcta
    12241 tatcgcccgt caggtcgatc ctgcggcatt aaccgttcat tacgtaacgg cgctgggaac
    12301 ggacagtttt agccagcaga tgctggacgc ctggcacggc gagaacgttg atacttccct
    12361 gacccaacgg atggaaaacc gtctgccggg cctttactac attgaaaccg acagcaccgg
    12421 cgagcgtacg ttctactact ggcggaacga agccgccgcc aaattctggc tggagagtga
    12481 gcagtctgcg gcgatttgcg aagagctggc gaatttcgat tatctctacc tgagcgggat
    12541 tagcctggcg atcttaagcc cgaccagccg cgaaaagctg ctttccctgc tgcgcgaatg
    12601 ccgcgccaac ggcggaaaag tgattttcga caataactat cgtccgcgcc tgtgggccag
    12661 caaagaagag acacagcagg tgtaccaaca aatgctggaa tgcacggata tcgccttcct
    12721 gacgctggac gacgaagacg cgctgtgggg tcaacagccg gtggaagacg tcattgcgcg
    12781 cacccatcac gcgggcgtga aagaagtggt ggtgaaacgc ggggcggatt cttgcctggt
    12841 gtccattgct ggcgaagggt tagtggatgt tccggcggtg aaactgccga aagaaaaagt
    12901 gatcgatacc accgcagctg gcgactcttt cagtgccggt tatctggcgg tacgtctgac
    12961 aggcggcagc gcggaagacg cggcgaaacg tgggcacctg accgcaagta ccgttattca
    13021 gtatcgcggc gcgattatcc cgcgtgaggc gatgccagcg taaacgcagc atatgcaaaa
    13081 ggctggataa agtgttaccc ctctatccag ctcatccata aatacctgat ggttgtgcta
    13141 actcatcacc atcaggcatt actgtgcagg tggaatatcc gtcacttcag gatgtacgtc
    13201 atccgtggcg acagaggtgg tcgcagcggc ggtagatggg gccatgattt gatcccaggc
    13261 cgcctgcaac gctttcatgt taaattccgg ctcgcctttc ggctgcagca gtattaacgc
    13321 catatcattc gacaactgct gacgcagatc ctgatttaac atctccacag tcaggctgtt
    13381 aaggaaatcc tgccgcagtt tctgatactg ctccggcgcg atatcgacga cctgattttg
    13441 caacgaacgc atccgctggc ccatcagaat atcggtatca gcccgtgcat aggcggcaaa
    13501 cagtttctgc agctccagtt tcttttgcgc cactaacgca ttgaactctt cttccggcag
    13561 acctttatcg cgaaccttcg ccagttcacg cgccaccaga ttaaggttgc tgttcagctt
    13621 gtcgtttggt gattcgatgt tgatggcaca ctgcgcacgc agatacagca cacggcagtc
    13681 aaatccaaga ccgatgtctt tgctgttact ggcgcttaac gcttgctgaa catgccagaa
    13741 cagcgcctcg cgggccaggt ccgcacgcca gtagcgcagc agtgcggctg attcacgaat
    13801 cggctgccac ggcgtatccc acatgataga taaccggtcc tgacgcaccg cgtcagtcat
    13861 aatactcacc gcttccgcac gcagcggaga aagcgtcggc accggagctg gcgtttcacg
    13921 tttgcctttc agttcgccaa acgttttgtt gatttggtcg acaaccgagc gcgcatccac
    13981 gtttcccacc accagtagcg tcattgcatc cggggtgtac catttctgat agaaatcttt
    14041 aattttttcc gcttcaacgg gttgtttcag cggatcggca ggatcgtgac ctaacaaggt
    14101 tgaccctttc agacgatagc gccaccagcc ctctttagta tcggcaggcc aggttgccac
    14161 catgtcctga ctttgcagcg cgtggttgat ggtttctggt gtgatggtca atttgccagt
    14221 ggcatttgcc agataagaga gcgcttcttt cagcaaatcg ttacggttat tgggcaaact
    14281 cagattaaac agcgtggtgt cataagagac aattaccggc ggcatcgggc gtttagggtc
    14341 gatcccctgc tgccacaatg aacgcgcctg tgctgcgtca aggccaccgc tttgcgttag
    14401 cgcaatacga gggatggcgt gactgtaacc gctctgttgt gtactttcgg cgagcgaacc
    14461 ggtattaacc agcaagcgaa tttcaacacg atcgctggga cgctgggggg tggtcagcac
    14521 ttgccactgt aaaccgttgg aaagcgtccc ctgttgccat gctggatcag gctggagcgc
    14581 atctgcctgc acatagccag cagtggccat catcagcaaa ccgcccgcta aaagtcgaat
    14641 ttttgtgccc tgcatgtgaa cccctgatca actatcctgg taataaaaaa actgcccgtt
    14701 gtcagtcagt ctaaatgacg ttaaaaacac ttcgtgttag accgcaagaa catgaaaatg
    14761 tcacggaaga agtgaaataa acctgaactc ccccaggtgg taagttcagg cacaggggtc
    14821 aattatgcgc aaacacccgc actcggggaa gggagtgcgg gcataagtgg tgagattaag
    14881 aggataattc gtgcgttttg ccatccggcg cacgattatt cagcacatcg tccagttttt
    14941 tgtggtccag ttctttcacc cacttagcaa cgacaatggt cgctacgccg ttaccgacca
    15001 ggttagtcag cgcacgagct tctgacataa agcggtcgat accgaggatc agcgccagac
    15061 ccgctaccgg caaatggccc accgcagaga gcgtcgccgc cagcacgata aagccactac
    15121 ccgttacccc tgccgcccct ttagaagaaa gcagcaacac gattaacagc gtgatttggt
    15181 ggacgatatc catctgactg ttagtggcct gggcgataaa caccgccgcc attgtcaggt
    15241 atatcgatgt gccatcaagg ttaaacgagt agcctgtcgg gatgaccagc cccaccaccg
    15301 atttgcggca gccgagtttc tccatcttgt cgagcatacg cggcagcgcc gactcggaag
    15361 atgaagtccc cagtacaatc agcagttctt cacggatgta gcggataaat ttgaagatac
    15421 tgaaaccagt cgctttagcg attgaaccca ataccagcac cacaaacagg atacaggtaa
    15481 tatagaaaca gataatcagc tgccccagtt gcaccagtgt gccgacgccg tatttaccga
    15541 tggtaaacgc cattgccccg aacgcaccaa taggtgccag acgcatgatc atattgatga
    15601 tgccgaagat gacctgcgag aaactttcga tcacgttaaa aatcagttgg cctttgctgc
    15661 ccagacggtg gagcgcaaaa ccaaacagta cggcaaacag cagcacctgc agaatgttac
    15721 cgctagcaaa tgcgccaatg acgctcgccg ggatgacatc cataatgaag gcgacaatgc
    15781 cctggtcttt cgcctgatcg gcgtaaaccg ctaccgcttt cgcatcaagc gttgccggat
    15841 cgacgttcat tccggcacca ggctgcacga cgttaacgat gataagacca ataatcagcg
    15901 cgatggtact gacaatttca aagtaaagca gtgcgactgc gccggtacga ccgaccgcct
    15961 tcatgctttc catgcccgca atgcccgtta cgacggtaca aaagatgaca ggagcgatga
    16021 tcatcttaat gagcttaacg aagccgtcgc caagcggttt catttgctcg cctatttcag
    16081 gatagaaatg gccaaggaga ataccaatgg ctatcgctgt caggacctga aagtaaaggc
    16141 ttttaaacag agaggttttc atagggtgtc ctttagtaaa accacaggtc ttgtaaggtt
    16201 atggggtacc tgcggcctta aaataacacc cagacaacat cacagaaatg tacctggatc
    16261 ataattgaaa caaaaaggtt aaaaagtttg agctggctcg cacaaaccag cactttttaa
    16321 agttttgtaa tcagtttggg gtagctactt ttcttcctgg taactctctt cgaagatttc
    16381 aatagggagt gggcgagcaa aaaggaagcc ctgggcaata ccaacgcccg cttttgccag
    16441 ccagtcgcgt tgtgcttcag tctccacgcc ttcggcaatc atttgtaagt tcaggctctg
    16501 cgccagcatg ataattgcag caatcatgct gctatcttcc ggcaagcctt caacaaacat
    16561 tttgtcgatt ttcagtacgt cgattggcaa cgatttcata tgctgcagct gacgcagccc
    16621 tgcgtagccc atgccgaaat catccagcgc cacccgaact ccggcattgc gcagcggacg
    16681 gaggattgcc accgcagcat gagggtcgtc aatacgtcgg ctttctgtca cttccagaat
    16741 cagtgttccc ggctgaatgc gatagcgggt taacagttcc agcatatccg ccaccatatt
    16801 tgggtgcatc agttgcagcg cagagaggtt taccgacaag ggcagcataa tgccgcgctc
    16861 ttgccaggct gcaagcaatc gacaggactc ttccagcacc cagtgaccga cggtaaccat
    16921 cagcccacag cactcaatgc gatcgattaa gccatccggc aggtcccaac tgccatccgg
    16981 ttgctggata cgcagtaaca cttccgcact gaccagttta ccgctggtca tctcgacctg
    17041 tggctgtaac caaatagcaa actgatgatt ttccagtgca ttaaggatat cgctctcttc
    17101 cgtcaaccgc ttctgggcgg cttccatctg ctgcggatca aagaactgaa tctgattctt
    17161 gcctttatgg cgagcggtaa atgccgcaga aatagcgcga ctgtaaagct gttcggcggt
    17221 gagatcgccg tagaacatcg ccacgccaat gctacagtgc ggacggagtt gaatacgttc
    17281 aatcggcagg cgctcgctca tgatagtgag cacttgctga cctaaggtga ttgcgtgcca
    17341 cggttcctgt acaccgttgg caatgacagc aaagtcataa ccgctaatct gcgcgaggat
    17401 catacgtggc gacagtaccg atttgagttt ttccaccagc gtcagcagca gaatttctcg
    17461 ttgcgcctct ttcagcacgc ccgcagtatc acgcagggtt tcacaggtga tgatcatcag
    17521 cgcggtggtt tgtttacgcg cgacaacctg ctccagcatc tccatcagca aggctttgtt
    17581 cggcaaatcc gacaccggga agcgcatcgc attttcgttc tgttcttcat aatggcgctg
    17641 cagcaattgc tggttgaggt tgtaactgcg caccaacata ccgatttcat cgtcctgatg
    17701 cagacgcggt aatgccagtt ggtgaccaac aagctcctgg gctgggatgg cgttaagttc
    17761 gcgagcaata ttgcgtaacg gatgcaaaat caggcggtta atgcaccagc tgatggcgac
    17821 ggtcaggata agcgacaaaa gtaagtaaat ggtcactaac gttgagaggg tgctcatcac
    17881 gaacttatac atacggaagg aatccgcctg tagtaccaga taggcaattg gctgcgggtt
    17941 tgccggacgt tcgagcgagt aaacgcccag cgagatttga accggtagct caaacaggcg
    18001 agtaaccatt accggcaccg ggcgctctgg aataaaactt ttacgcagcg cctggaactg
    18061 gttaggcagc actacatcgg cacggctgac cacgcctgcc ggtttaatgc tggcaagaat
    18121 agcttccgct tcgggaatat cgcctttcaa aatagctgaa gataagggtt cgcggacaga
    18181 gcgagcaatg ctttccagtt gcgtagccgt gttatagcga ttctgctgga ccagatggaa
    18241 cagcaaaacg gtgcaaaaaa taaaaacgaa caccaggaca acggctgcca ccattgccat
    18301 ctgcttgatt gttaacgagc gacttacgcg caaattgtct ccccccggaa atcccagatt
    18361 gccgcccgag tatacccgat cgcagcggca ttaagagagg cgctatctga aaacttacca
    18421 gtcggcgtaa ggtatcagcg gctgcggcgg taaatccatg tcaccctgcc atccggcggc
    18481 ggaataacgt acgtagagca ggaaatggct gggtgcgtaa tctttcgcct gctggatatc
    18541 aattgccgtg ccaacaaacc agttggaagt aacacgtcgt tcaagtaatg cccgcgccgt
    18601 gtagccgaag ccctgactgc tgccgccatc gttggattgc cgcgcagctt cttcctgcca
    18661 gtcggtcggg atcagattca tcagcggata acgcggcatg gttttggtgc gtgattgcga
    18721 ccacgagcca gacgccccca gctcccacga ccagttttcc gtgcgctccc gccacatcac
    18781 cggtatggca aacgacaggt attcctgcgg actgtagtaa ccgccctgac cgagtgagta
    18841 gccactcaga tctttgtcgt aatgccagat catgttgttc aggccgattg tgacgcggcg
    18901 attgttctgg ttaatgacct tatagtaata gcccgtcatc cagcgcacgc gccagttatc
    18961 ttcgacattt ttaccggtta actggtcgcc actaagcgat gcccagacgc cgtttgcttc
    19021 acctttatcg tagctcagac ttagccccac accgtcggca cgtacgccac cccatttttt
    19081 cccggtattg ctcggggagt ctttttgccc accaaaggcc agcaaagaac tggagatggg
    19141 ccgacggtgg gcgttaacgg tgtaacccag cggcccgata tcatcgctgt aactgatgcc
    19201 gccgaccaca tccaccacgt tgaagcccat cggcgtggta ccgatatccc agctccagac
    19261 gtcatttcgc cagccgaccg ccacgctggc accggagtcc gactggctgc ggttgccact
    19321 acagtcctgt aatgtacagg tgccccagtt gtcatcccat ttgccatcgg cattagtgga
    19381 gaaactgccg acgttcatat tgacgaaatc actgcgaaag aacatccgcc cgtcagaata
    19441 cggcgcatcc acctgcaaca tggtagtgtg cgctttcaga tcggagtaac caccggtgcc
    19501 gctcgaaccc cagtaatcgt gctcgagggt gacgttaaga tcctgctggc gatagaggtc
    19561 cgccgcatcg ctgcgcacgc cgcgtttcag ccagtcatct ttctcgtcgt tacgggtcag
    19621 tcgggtgaat gtgtcgttat cctgcggacg cgtcgtagtc acaccggatg cgaccatggc
    19681 gtctttgtag gtttccagcg cctgctttgg atcgcccgcc tgcgcttcaa acttcgcacc
    19741 gtcgcgcagc accatcgcgc tttccatcga cggtggctga gattttgcct gcgggatcaa
    19801 cttattaaac gtctgctgcg ccgctgcggt atctccaagc tgcgcctgcg ccagcgccac
    19861 gcgccgctgt gtgttcagcg aggcgttatc ggtagcgggc agtttcgcca gctggctacg
    19921 tgccgccgct gtgtcaccgg cagcaatatc cacttccgtc agccccagaa tggcatcggc
    19981 gttagttggc tcccgcgtca ggacattctg atatgcagcg cgggcggcgg tgtaatcacg
    20041 tcgctgctgc gcccagtcag ccagcgtgag gtcaatacgc gtggaaggtg gttgctgacg
    20101 cagcatcgct tccgcttcag cttctttgcc gctttctcgc aggcgattag cggtttccag
    20161 cacctgatcg ctttgcagtc gattaaccag ctcctgaata ttgctgttcc actgcgcgcg
    20221 cggcaggctg ttgatatgcg ccagcgccgc tctgtcctgg tcatgaccag agaggtacag
    20281 cccgtaagcg taaacctgct ccgggtcgtt cggcttctgc tgcgccagat tgcgcattaa
    20341 cgtatcggcc tggctgcgtt gtccggcctg ccagagatcc tgcgaaagtc ggtaagtaat
    20401 ccatacgctg ccggggtcca gcgccagtcg ttgccgctga agtgctgccg cctgcgccca
    20461 tttgccctgg ttttccagtg cctctgcctg ctgtgccaga cggtcgtttt gcaggctgcg
    20521 ttcgatatca tcaatgctac gccgctgact ggcagagagc gaggcgataa acgcttcagc
    20581 tttttctggc gattgctggc ggtaaatatt ttccagcccg cgcacggcgt tagtgttgcc
    20641 gctgtccata cgcaaggtct gctgataata acgttcggcg gcgggataat cttttcgcgc
    20701 catcgccaca tcgcccagcc ccagcactgc ataactgtcg gtgttatcga cattacgcgc
    20761 ctgctggaac aggcgttctg cccggtcagg attattggct ttcagcgcag catcgccctg
    20821 ctggatcgcc agccagtagc ggtttacttt cagcagactg ttccatttgt cgttgttgct
    20881 gctgtgcggg tccagtgcga gggctttttc cagattcgcc actgcattgg cgcgatcgcc
    20941 tttctgagaa tacgcctggc ccagcgcccc cagagcttca ctgtctttcg ggttcgcccg
    21001 caccgcctgt tgtagttcgg gaatggcttt acccgccata ccagagtcca ccgccgctaa
    21061 accttgcgca cgagcgcgga aagcaggatc ggccagctgt ttttgctgtt ctgccagttg
    21121 cgattgcgca gccgccacgc tatcgccatc actaaagatc gagagatatt ttttcagcgc
    21181 cgacacactg gcatcactga cgggcatgtc tttaatctgc ccgtaccaga ttttagaggc
    21241 cccttcgcgc ccggcgttcg attttgccat ctgttccagg acggcaaaac cttcgtcacg
    21301 gcgatcgcta ctaaacagca atagcgccag attgttttgc aggcccgtat tgcccggtgc
    21361 atccgcattg atgcgtttta actgattaat cgcttcgcca cggcgagccg gaattttcgc
    21421 caccgtactc cagtactcga cagcaatgtc accttccggt ggcgcaccgt tgaacagttt
    21481 gttgtaactc gccacagctt cttctgcatg accggtcgtc gtctgcaatc gtgcctgttg
    21541 cagtgcctga cgaccatccg gcgtggaaag tagcatcgta gtccgcgacg atttatacgc
    21601 atttgaactc ggcgctaact gcgacagccg atcgagctgt ttttgcgcgc catcaatatc
    21661 gccctgacgt aacaaagaac ggaaacgggc ggcaacgacg tccgggttat tcggatcaat
    21721 aagttccagc cgatataacg actgttgcac cagatcttca cgatgggtcg cttcgcctaa
    21781 ccgaacttgc tccagcaact gttgctgagc ggttggtgct gcctcgacca tcggcatgac
    21841 ggccagaccg agggaaagcg tgaatatgtt tagtgtgaat ttgcgcattc ctggccccag
    21901 tcaggtaata actcaccttt tgtcgagaag cggaaacggt gttgatccca gccttgtcca
    21961 aacagggtca gcacatagtt gtaataggca tcgctgccgg gaaagttatc ggccacgcgc
    22021 tggcgctgaa cggcctgcgc atcgcggttt tgtaaaaagg gcagcatggc ggcagaaaaa
    22081 ccgactggtc ctttaccctg cgctttcccc gtagccacat ccactttttc cggcggataa
    22141 ccgtttttct cagtgaatgt cgccatcggt ttaaaccggt tgagcatccg cgctttctgc
    22201 ggatcgctgt caggcatcat gcctacccac atgtaaacgc ggatggcgtc gtagctgctg
    22261 atcaatgttt tttcggcttt tagctgccag cctttgtctt tctcataacg cacccagtct
    22321 ggcgaaaagc ctttcggggc ggtttccagc aataaacgtt gattggtttc gcgcagcgta
    22381 gtccacggcg cgccaaagcg ggtgaaatac tgcgccagcg tcggcggcag gtagctgggg
    22441 ttaaaacgcc agctgttatc ctcagcaaaa cccactttgc ccggtaacaa catggaaccc
    22501 agcccaggca ccgtcaccac ttcctcccgc gcgatacgtt ttagcaacgc gctgccgatg
    22561 tcggtataac gctgctcttt ccacaaacgc cccgcctcca gcaacgacca ggccatccag
    22621 acatcaccat cggaggccga attgctgtcc agcacttccc acttactgtt ctctttcttg
    22681 ccccacagcc aggcgggcaa acgttctttt aaagaaccct gagcgagatt gttctgcgtc
    22741 cagtcgagaa tattatcgaa agctgcacgg tcgttagccg ccagggcaaa gaacataccg
    22801 taactttgcc cttcggaggt ggtgattttg cgcgcgtcgc tggggtcgat gacgcgccct
    22861 tcctgactga tgtaatcctt tttaaactgc tcccaggcag gccaggtaca ggctgcctga
    22921 acactaaagg cagccaacaa cagcatcgtc acgattccac tacgcaacac attcatcttc
    22981 aattactcgt tatccgggtt aagacgacga cgactaataa ttcgcagcag acgccacagt
    23041 acccatgcca gcaatatcac actgatagcc gccagcaccg ccagcagaat cggatggttt
    23101 gccagctcat accacacgcg ctcgaaccac ggcagatgac ctacgtaata aacgtcgcca
    23161 acacgtaggc tgttgatacc ggactcgcgg atcaccgcga ccgaaccgaa catggtggcg
    23221 cgtttgccgc tatcgttcac cgcatcgtta agcatttcat aaccgcgtgg gctatctgcc
    23281 aacagcgcaa tcacgctgcg ctggtcgtta tacggcgact ggaagccaat caccgccgcc
    23341 atcgcaccgg aagaggtcag cgttgaccgg gtttctgccg cgcgatcgct ctcgtccggc
    23401 acaatgccgg ggaacggggt ctggcgcatc ggtgttttca cccagctttc ggtcgcctgc
    23461 accaataggt cgatctgctt atcgtctttc agtttgtccg ggataccacc gatgatcatg
    23521 atgtcggcat ctttgccctg aatggtgctg ccatcatcgg tcaccgtcag attaatcgcc
    23581 gggaagcccg tctgtgcgcc gataaaacca acagtattca gcaacgtttc catctgtgct
    23641 tcgttaggcg ctttcggcat cacggtgatg gtttgcgaca gatccgccat ccggctgaat
    23701 gggaagcccg cgttagcaaa ggcgcgtaga tccggcatcg ggatgaagtg gtaatacttc
    23761 gagaagtcga tggtggagtc gtcaccaatc accacatgat tctgcaccgg ctggaaggta
    23821 atacagttat ccaccgaacc gcccggcatc gggttcatat actcaaagtc gaagcgcagc
    23881 tggttggtcg cgcccagttt cagcgccgga atagagacat ctgttttgcc atccagcaaa
    23941 ccttgtaata ccggaatccg cagcagcagg cggttcgcct cctgtttgct gctcaggttg
    24001 aaggattgca ggaactggtt attcaggctg atatccatcc gcgaactgtc tttcaccggc
    24061 ggcatggtgt agcggtaatt aatatccata tcaatgccgg tactgcgcat caggtagaga
    24121 tccggcggca ggtttagcga aacgttaatc gctgctggct caagaccgct ggattgtaac
    24181 tgttcttcat aggttttcag ttcgccaaat gtgaccggac gatcggtacg tacccagttc
    24241 ggcgcatcgt acggcttacg cggtagcagc ggtttcactt cattcactac cacgctttca
    24301 ccacggaaca gaatgttacc ctgagcgata cctttcgctg cctgcaacag gtctttgtcg
    24361 tcacgaccaa acaccaccag cagtttgacg taaggattct gcggatggtt aatcatctca
    24421 atcaccgggg cttttaccgc cggatgatcg cgcaggaagt ccggccgttt gtcgttggtt
    24481 gcaaagacaa tggcattacg atccggcagt tggttataga gcaccgggaa gttctgccca
    24541 cgccagccag aacgcgaacc aaaccacgag gcgacaatgg cagaggcttg ttgcagccca
    24601 acatccggcg cacccgcaaa gaccatcggc aaggtgttag tacggttatc gcgcgggtca
    24661 aagaatggca ccgggaagtg tgacaggtca ttcttcacat tcagggtctg ataggtcaga
    24721 tccagtccac tgctccgccc aacatccagc caaagcgtgg tgctggccgg gttttcgcac
    24781 acgtcctgat aatggccgac aaactccagc cgtacacggt tgaagtcggt aataaacagt
    24841 gggttaatgg gcatttgcgc cagcgttttt ttacccaact gttctttggt cactggcagc
    24901 acgcccatca gttcatcatt gagataaacc tttaactgcg actggacagg cagtaacgat
    24961 ggcgatgggg tgtattcgag gttgagcatc gccttcgtca ccacttcatc gctgcgcata
    25021 ccaaactcaa tgctgccgtt cgggttaatg ccacgtagca ccatgctgcc cggcggcggt
    25081 gcaatttgcg caaaggtcag cttcacatca cgcgaaggac cgttctgcgc cacgactggc
    25141 gcatcagcac cctgcacgcc cggcatcact tgccccacct gcggattttg ttccgtctgg
    25201 gcggctacag ctggctcagc attgatcagt ggttgcgttg ctggcgtcgc ctgcgtcatg
    25261 aaagagggga acgcactcat ccccatagcc actgcacaaa tccagaatag ttttcttttc
    25321 atcgcgttat catcattgtt gagccaaagc ctgatccgat ggttgtgccg tttcgctccg
    25381 ctccgggcgg cgcggaataa acgatacaac ccaggaaacc agagaagtca gcacacggaa
    25441 tatgcccttc accgaagaag gcgcaaactc cgccagatgg cggtagccac ggaagccgag
    25501 cttcagaata tccagcagac tttccagcgg cttatcttcc gggtagctgt cctgccagag
    25561 cgcccatgta tccgcacggg caaacgtaca ctgcacaaaa tcgatatgtt gctgggtggt
    25621 gagcggcatt aatttcagcc caacgtcatt acccatcacg cgcgccacct gggtcgggaa
    25681 gacgtattcc tgctgaccgc gtttaagcaa cagattcact ttctgccctt ccagaatctg
    25741 cgcctgaccg ttgatcttga tccccaaacc accgtcggag aaatcctgaa cggtacacga
    25801 gaagaggtga ccatcttcgc gggcaattgc cgcgggcatc gtcatctcca cgcggtgcga
    25861 tcggcgtacc tgtttgcttt ctaccgatac tgcaactgcg ccgccaagaa caatcaggtt
    25921 gtagaatacc cacaccatac tgacgaccac ggtgagcatc tcggttggcg ggccatagaa
    25981 gtagcgccag atgcctaccg caacgcccac caggttgagc aggacaagga agatgtaggg
    26041 ccgcgagatc acccagtcga cgtactcttc ttccaccagt ccacctttgg cggtgacgtt
    26101 aaatttgcct ttgtgcgggt taatcagcgc caccagcgtc ggtggtgcga tataccacgc
    26161 cagcaccgtt tcgtagattt cactccagaa agagtggcga tatttgccct ggatcttgga
    26221 gttggtcagg ctggcatgga tcatatgcgg cagcacgaac agggcgatca tcaacgctgg
    26281 cgcatagatg atgtaggcat gaagcagcag gaacgccagc ggcgcagtca ggaagatcag
    26341 tcgtggaatg cccgacaaga agtggaacat ggcgttgacg tagcacagcc gctgagcaaa
    26401 cttcagccct ttaccggtga gcgggttatc gagacggaag atttgcacca tcccgcgcgc
    26461 ccagcgaata cgctgaccga tatgcgccga cagactttcg gtcgccagcc ccgccgcctg
    26521 cggaatacgc atatacgcgg aggtatagcc acgacggtgc aaacgcagag aagtatgcgc
    26581 atcttcagtc acagtttcga cagcaatgcc gccaatttca tccagcggct tacggcgaat
    26641 caccgcacag gaaccgcaga agaaagtggc gtcccacata tcgttgccat cctgcaccag
    26701 accatagaac agcgtgcctt cgttcggtgt tttacgaaaa cgccccaggt tgcgttcaaa
    26761 cgggtccggc gagaagaagt ggtgtggagt ctgcatcatc gccagctgtt tctctttcag
    26821 gaaccagccc atggtcattt gcaggaacga tcgcgttggt acgtggtcgc agtcgaaaat
    26881 cgacacgaac tcgcctttgg catatttcag cgcattgttg atgttgcccg ctttcgcatg
    26941 ttcatgagtg gtacgggcga tatatttcac ccccacgttt tgcgcaaact ggcgaaactc
    27001 ttccctaccg ccgtcatcga ggatccagat gttcagcttg tctttcggcc agtcgatacc
    27061 cagcgaggcg taaatagtat ttttcaccac gttaagatct tcgttgtaag tcgggacaaa
    27121 gatatccacc gacggccaca gcgacatatc tttcggcaat ggcaccggct gaccggatcg
    27181 tcccagttca gcgtagaggt gtaacgccac cagatataac ggcaagaaac ggtcagcgac
    27241 agcacaatca acattagcgc cgagaagcgc cccggcatcc gccgtacgat cagcgctaca
    27301 ccccacagca gcatcaggaa gataaactgc gccagcgggt taaacggttg agtaacgcag
    27361 attaacgcca gaatcagcga gaaggtgacg atgataccga ggatcaaccg ccgcgccccg
    27421 gcactcaaat gaccgagctc ttttttctca tccagatgct gtgttttatg gctaacgcgc
    27481 tcaggcagct cgttcatcca ttgatggtaa cgtccacgaa tattttgcag acctgaaaat
    27541 gcccgcctgc gcggtttcgg cgtttctttg cgcgatgcac cgatcaataa ccagcatgtt
    27601 tgaatgagat aacggaccgg gtccagcgga cgcggacgcg aggcgttgat atgcggatac
    27661 aggtttttat gttctgcgcg aatacgctgc cagcgcgggt gctccagcgg aataaaaatc
    27721 caggccagga tcatccagaa acagccgagc gtcgcgctga aagccgacgc accgtgacga
    27781 cgataatcgc gataacgccc gataagccgc gcgttgaccg gcgggataag caaccaccgg
    27841 gtcaggatac tcatgatgca ctcccgactg gcgttttcag cccggagtag ttcaacaggc
    27901 accagttcgc cagcgtcagt atctcttcag ccgccagcgc atcactgcga tattcaccta
    27961 ctggttgctt agccgccagg cattcagcca tcgcttcatc acgatgaatg agcatcggca
    28021 gtaatcggcg ctggctttgc aaccaaagct ggtaaatatc gtcctgaacc tgactgccaa
    28081 tacggaagtc attaatcaaa atatgtgcgc catccggcag cgcttgctga tgcagtcgga
    28141 tatggcagtt ggcatccaca ttgacgattg ccagcgagtg atcgcacaaa ctcagcagct
    28201 ggtgggttat ctgcgaggca tcacgcggta agtcgattaa aatccactgg taacgcccgc
    28261 tggcttttat ttgctgtaag ccggagcaaa tatcgctcag ccgggtttgc cagtgctgtg
    28321 gattttcttg ttcttcaatg gataactgac caaaaggcag caaatcgagc tgcgaggtgt
    28381 agcgcaaccc agcgtcacgc cagtcctggc catccagcat cgctctggcc cagccctgac
    28441 ggtgggtaaa atcaacatta aatgacaggc gcaacaagtt gtccgggcag gcatcgacca
    28501 ccaggacatt ttctcccaac atttgtaatg accaggctaa tgcggcggtg atggttgt
//
LOCUS       DAZKHC020000058        28239 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZKHC020000058 DAZKHC020000000
VERSION     DAZKHC020000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.058"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            63..464
                     /locus_tag="ACHOSM_RS14335"
                     /old_locus_tag="pgaptmp_002867"
     CDS             63..464
                     /locus_tag="ACHOSM_RS14335"
                     /old_locus_tag="pgaptmp_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559350.1"
                     /translation="MFAIKQEINNATSLFEIESITVGYPGSDQFKQAFEIAEELGIKT
                     PDAIEYIPVAYEDEEMTKAIGEEQTLSTERKDVQRDDCIAVICSGVASEMFPDLPAMG
                     GVGYQFLYKGDTLRIYTSSLLIEEVGTENDN"
     gene            791..1651
                     /gene="repA"
                     /locus_tag="ACHOSM_RS14340"
                     /old_locus_tag="pgaptmp_002868"
     CDS             791..1651
                     /gene="repA"
                     /locus_tag="ACHOSM_RS14340"
                     /old_locus_tag="pgaptmp_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HGU4559351.1"
                     /translation="MNQSFISDILYADIESKAKELTVNSNNTVQPVALMRLGVFVPKP
                     SKSKGESKEIDATKAFSQLEIAKAEGYDDIKITGPRLDMDTDFKTWIGVIYAFSKYGL
                     SSNTIQLSFQEFAKACGFPSKRLDAKLRLTIHESLGRLRNKGIAFKRGKDAKGGYQTG
                     LLKVGRFDADLDLIELEADSKLWELFQLDYRVLLQHHALRALPKKEAAQAIYTFIESL
                     PQNPLPLSFARIRERLALQSAVGEQNRIIKKAIEQLKTIGYLDCSIEKKGRESFVIVH
                     SRNPKLKLPE"
     gene            2209..3405
                     /locus_tag="ACHOSM_RS14345"
                     /old_locus_tag="pgaptmp_002869"
     CDS             2209..3405
                     /locus_tag="ACHOSM_RS14345"
                     /old_locus_tag="pgaptmp_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4559352.1"
                     /translation="MSDSSQLHKVAQRANRMLNVLTEQVQLQKDELHANEFYQVYAKA
                     ALAKLPLLTRANVDYAVSEMEEKGYVFDKRPAGSSMKYAMSIQNIIDIYEHRGVPKYR
                     DRYSEAYVIFISNLKGGVSKTVSTVSLAHAMRAHPHLLMEDLRILVIDLDPQSSATMF
                     LSHKHSIGIVNATSAQAMLQNVSREELLEEFIVPSVVPGVDVMPASIDDAFIASDWRE
                     LCNEHLPGQNIHAVLKENVIDKLKSDYDFILVDSGPHLDAFLKNALASANILFTPLPP
                     ATVDFHSSLKYVARLPELVKLISDEGCECQLATNIGFMSKLSNKADHKYCHSLAKEVF
                     GGDMLDVVLPRLDGFERCGESFDTVISANPATYVGSADALKNARIAAEDFAKAVFDRI
                     EFIRSN"
     gene            3422..4423
                     /locus_tag="ACHOSM_RS14350"
                     /old_locus_tag="pgaptmp_002870"
     CDS             3422..4423
                     /locus_tag="ACHOSM_RS14350"
                     /old_locus_tag="pgaptmp_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HGU4559353.1"
                     /translation="MSKKNRPTIGRTLNPSILSGFDSSSASGDRVEQVFKLSTGRQAT
                     FIEEIIPPNQVESDTFVDQHNNGRDQASLTPKSLKSIRSTIKHQQFYPAIGVRRATGK
                     IEILDGSRRRASAILENVGLRVLVTDQEISVQEAQNLAKDVQTALQHSIREIGLRLMR
                     MKNDGMSQKDIAAKEGLSQAKVTRALQAASAPEELVALFPVQSELTFSDYKTLCAVGD
                     EMGNKNLEFDQLIQNISPEINDILSIEEMAEDEVKNKILRLITKEASLLTDKGSKDKS
                     VVTELWKFEDKDRFARKRVKGRAFSYEFNRLSKELQEELDRMIGHILRKSLDKKPKP"
     gene            4649..6355
                     /locus_tag="ACHOSM_RS14355"
                     /old_locus_tag="pgaptmp_002871"
     CDS             4649..6355
                     /locus_tag="ACHOSM_RS14355"
                     /old_locus_tag="pgaptmp_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559354.1"
                     /translation="MSNLREYQNRIADIAKRSKAVLGWASTAQFGTDNQFIKDDAARA
                     ASILEAARKDPVFAGISDNATAQIATAWASALADYAAAHKSMPRPEILASCHQTLENC
                     LIESTRNSMDATNKAMLESVAAEMMSVSDGVMRLPLFLAMILPVQLGAATADACTFIP
                     VTRDQSDIYEVFNVAGSSFGSYAAGDVLDMQSVGVYSQLRRRYVLVASSDGTSKTATF
                     KMEDFEGQNVPIRKGRTNIYVNRIKSVVDNGSGSLLHSFTNAAGEQITVTCSLNYNIG
                     QIALSFSKAPDKGTEIAIETEINIEAAPELIPLINHEMKKYTLFPSQFVIAAEHTVQA
                     AYEAQREFGLDLGSLQFRTLKEYLSHEQDMLRLRIMIWRTLATDTFDIALPVNQSFDV
                     WATIIRGKFQTVYRDIIERVKSSGAMGMFAGADAASFFKQLPKDFFQPAEDYIQTPYV
                     HYIGTLFGNVKVYEVPAGICKNLTTENIQFSSMDVLCYVRDENPGKAGFVTGDAVPAI
                     PFQHPTTPALVNRTTLWGSAINDMHPRNGADYFTRVTLTMAKKGGLNFISGDTIDAGD
                     SE"
     gene            6416..8005
                     /locus_tag="ACHOSM_RS14360"
                     /old_locus_tag="pgaptmp_002872"
     CDS             6416..8005
                     /locus_tag="ACHOSM_RS14360"
                     /old_locus_tag="pgaptmp_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559355.1"
                     /translation="MSQYSIQQSLGNASGVAVSPINADATLSTGVALNSSLWAGIGVF
                     ARGKPFTVLAVTESNYEDVLGEPLKPSSGSQFEPIRHVYEAIQQTSGYVVRAVPDDAK
                     FPIIMFDESGEPAYSALPYGSEIELDSGEAFAIYVDDGDPCISPTRELTIETATADSA
                     GNERFLLKLTQTTSLGVVTTLETHTVSLAEEAKDDMGRLCYLPTALEARSKYLRAVVN
                     EELISTAKVTNKKSLAFTGGTNGDQSKISTAAYLRAVKVLNNAPYMYTAVLGLGCYDN
                     AAITALGNICSDRLIDGFFDVKPTLTYTEALSAVEDTGLLGTDYVSCAVYHFPFSCKD
                     KWTQSRVVFGLSGAAYAAKARGVKKNSDVGGWHYSPAGEERAVIARASIQPLYPEDTP
                     DEEAMVKGRLNKVSVGTSGQMIIDDALTCCTQDNYLHFQHVPSLMNAISRFFVQLARQ
                     MKHSPDGITAAGLTKGMTKLLDRFVASGALVAPRDPDADGTEPYVLKVTQAEFDKWEV
                     VWACCPTGVARRIQGVPLLIK"
     gene            8015..8830
                     /locus_tag="ACHOSM_RS14365"
                     /old_locus_tag="pgaptmp_002873"
     CDS             8015..8830
                     /locus_tag="ACHOSM_RS14365"
                     /old_locus_tag="pgaptmp_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559356.1"
                     /translation="MSKNFFQSGAFLGNGLSRFALNSDPVQLMESARASAEPPTDPVI
                     NNNPEPAAQTNDNVPSAPDPEQILEGKDGKEWTVEQAHQMILEAANRSAMQNALSDAA
                     DAVFAWADSGDLTFDSLDGFVQAIAGISDDDDSEVTEEQDDAYNEAWANVADFLAACG
                     VDDDLIEALADDEDDDAAADVGASIAGLDSDDRDELEAAFVVAGTSDEMLTEAFKKVV
                     RNGEIKLIRKRLRKKRLTAAQKSALKKARRKAQTGAAKLARKKSMKLRRKRLG"
     gene            8983..9447
                     /locus_tag="ACHOSM_RS14370"
                     /old_locus_tag="pgaptmp_002874"
     CDS             8983..9447
                     /locus_tag="ACHOSM_RS14370"
                     /old_locus_tag="pgaptmp_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559357.1"
                     /translation="MNMAQNWEAPFTGMSMGSVSGALGGFVQVGTETTSVARWNSLMV
                     WEGGTPPTFTLPVTFIALNNPFIEVSGAIAALTAMISPELKAANVGGRIPERVTLNIG
                     RRINITDVAIQDLSFDLDAPRDSNGYFLKNTVNLQLTGSSIYNSSDIVRAFQ"
     gene            9459..9968
                     /locus_tag="ACHOSM_RS14375"
                     /old_locus_tag="pgaptmp_002875"
     CDS             9459..9968
                     /locus_tag="ACHOSM_RS14375"
                     /old_locus_tag="pgaptmp_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HGU4559358.1"
                     /translation="MGHNNTKGNRKFIKGRYTANAAKGERLVSSEFQLTFAGHEDISV
                     LVRTSQIPEMTREDVEDYGPNGVKFNQHGPIRNSGEIQVQCVETIEGDILQFIKDRIA
                     AKDYVDITMAATPESKSSGVNAVTKAATTIEMLDCKIYSDAIDFSTEDVTAAVRPSLR
                     IVYNWIEWD"
     gene            complement(10085..10240)
                     /locus_tag="ACHOSM_RS14380"
                     /old_locus_tag="pgaptmp_002876"
     CDS             complement(10085..10240)
                     /locus_tag="ACHOSM_RS14380"
                     /old_locus_tag="pgaptmp_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4559359.1"
                     /translation="MKQQRAILIALIVICLTVIVTALVTKKDLCEVRIRTGQTEVTVF
                     TAYESER"
     gene            complement(10422..10667)
                     /locus_tag="ACHOSM_RS14385"
                     /old_locus_tag="pgaptmp_002877"
     CDS             complement(10422..10667)
                     /locus_tag="ACHOSM_RS14385"
                     /old_locus_tag="pgaptmp_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559360.1"
                     /translation="MQSDFLQLAIAFAGYVCIGFCVYMISRKMLVDIDRKEQAEEILE
                     WIFFGAVWPLGIMFAATFLLMWIFTLPGDFYRKKARH"
     gene            complement(10718..11563)
                     /locus_tag="ACHOSM_RS14390"
                     /old_locus_tag="pgaptmp_002878"
     CDS             complement(10718..11563)
                     /locus_tag="ACHOSM_RS14390"
                     /old_locus_tag="pgaptmp_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGU4559361.1"
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCA
                     NLAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANL
                     YRFTPSFLAFAQQAKNALIESKLKISSAATKVKAVLAKTLALFNFLSTPPCQNDTPSP
                     CQDDVAIKNKKSQVKKTERSVSGGAGTTRLKNLTSWIAEAKAKADNLRLSKKRAQKHE
                     FKQKVEAAARKYAYLKNKHSPDIGGVSNFDNLPHCMTVNEALNAVLAKNKDNEQWGIP
                     AGFRG"
     gene            complement(11593..12393)
                     /locus_tag="ACHOSM_RS14395"
                     /old_locus_tag="pgaptmp_002879"
     CDS             complement(11593..12393)
                     /locus_tag="ACHOSM_RS14395"
                     /old_locus_tag="pgaptmp_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU4559362.1"
                     /translation="MQQTFNADMNISNLHQNVDPSTTLPVICGVEITTDRAGRYNLNA
                     LHRASGLGAHKAPAQWLRTLSAKQLIEELEKETMQNCIVSFEGRGGGTFAHELLAVEY
                     AGWISPAFRLKVNQTFIDYRAGRLQPAIPQSLPEALRLAADLAEQKQRLEQKMLMDAP
                     KVEFAERVATASGVLIGNYAKVLGLGQNYLFTWLRDNGILIATGERRNVPKQEYISRG
                     YFTLKETVIDTSNGSRISFTTRITGKGQQWLMKRLLDAGVLVPVAATR"
     gene            complement(12558..13595)
                     /locus_tag="ACHOSM_RS14400"
                     /old_locus_tag="pgaptmp_002880"
     CDS             complement(12558..13595)
                     /locus_tag="ACHOSM_RS14400"
                     /old_locus_tag="pgaptmp_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU4559363.1"
                     /translation="MKKPLVTRNDITEAIALHTACMPTREIPGAIANYFMITRRFYTR
                     TDKAVINKLLIAEIRDYLIEQGRLRYATVAAEMRKEAHRMTGNNLNVEKPAPVASATP
                     APAVNIIPNTGDTIDSLTLLKMVNEARKLCGEPEVRNNKFIEKILDELDGEHYTKSVV
                     EKMNKTSMLVITMTFKQALRVAARESKAVRRSLIDKLEELQQANSPTPSIPQTLPEAL
                     RLAAELAEQKMQLEQQLVAAAPKVDFADRVSVANGILIGNFAKVVGLKQNALFSWLRQ
                     NGILMAFGARKNVPRQQYINAGYFTVKEVVLDDENGYQIRLTPQLTGKGQQWLTRKLL
                     DAGLLKPVAIG"
     gene            complement(13592..13813)
                     /locus_tag="ACHOSM_RS14405"
                     /old_locus_tag="pgaptmp_002881"
     CDS             complement(13592..13813)
                     /locus_tag="ACHOSM_RS14405"
                     /old_locus_tag="pgaptmp_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4559364.1"
                     /translation="MVNANPCARQEFIWRFYSCKKPHYHFVIAATEDEARSQLPDGPC
                     IFTARFSTNSRNSLSYWNLPFSADVQGGL"
     gene            14241..14888
                     /locus_tag="ACHOSM_RS14410"
                     /old_locus_tag="pgaptmp_002882"
     CDS             14241..14888
                     /locus_tag="ACHOSM_RS14410"
                     /old_locus_tag="pgaptmp_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001228780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559365.1"
                     /translation="MRKLLLPLLFMAGTVNAASSVKEICTDYTKYLGHVYGFAVSQDE
                     SMRKKLLSDMKRLKLSEAMVQQELYKVATNENAKYQYSRLLNPDANEINRSSFDYMVK
                     ACETAPDFAIPSWGVLVASNAVNKEDVGRNGIDSIRNAPGMRHQNVQGTLEERARGPG
                     TNSPMGNLSPEELQEYNQRMEQYEKAAREQMEQQKNGSLNTFQQGLKALNLPYEW"
     gene            14950..15426
                     /locus_tag="ACHOSM_RS14415"
                     /old_locus_tag="pgaptmp_002883"
     CDS             14950..15426
                     /locus_tag="ACHOSM_RS14415"
                     /old_locus_tag="pgaptmp_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559366.1"
                     /translation="MRINRFFLILALLTPFSSMANVSKWSTGETHGVRSYAVSSKDNY
                     TLTFECDVGFNNTDPNQVGTRLLTLMKTEPGGESFDAKKEQITLKVGDDEYPISSIGS
                     SVGDSYWYGFWSDTPDMEVKTFDAYVDGKKIATFTLRKAAELFNAAPEDGCLKRAK"
     gene            15485..16051
                     /locus_tag="ACHOSM_RS14420"
                     /old_locus_tag="pgaptmp_002884"
     CDS             15485..16051
                     /locus_tag="ACHOSM_RS14420"
                     /old_locus_tag="pgaptmp_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559367.1"
                     /translation="MTPRQLLEDVKSRFTPLIADEPALLESLLRKALGTYQDRAGHIK
                     RIRITDQASKSLACPVDFLALVSVTDHTGDLVYSDVYDGNIELEDTHRAVYPLNVSYL
                     ANLRDMDLDNGDVPPEIIGLLSDYLEVLIAIPNTDRLRRISIAGKLDASNLSDENTLY
                     QRKLDLEEKMSATRAIIPGIVLFSSMLK"
     gene            16062..16673
                     /locus_tag="ACHOSM_RS14425"
                     /old_locus_tag="pgaptmp_002885"
     CDS             16062..16673
                     /locus_tag="ACHOSM_RS14425"
                     /old_locus_tag="pgaptmp_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4559368.1"
                     /translation="MGLNVASVKSYVSSALTTTLFGSGVGEREVGKLTSIIMNKMLFA
                     QGWQFSVEVDGLEGADFFAKDITYHDYSIEYETIKIGGGNILQPTERSPGQITMMVRD
                     TVDGLVLDWFKTAKGRVINPDGTGNIPSKYLLNVRIYRLLSSGLTKLENEMTVFPVTT
                     GDVTYARDQVTEFKSFPMTFALHSTFNQSSSSLASLLGFSFSL"
     gene            16688..17569
                     /locus_tag="ACHOSM_RS14430"
                     /old_locus_tag="pgaptmp_002886"
     CDS             16688..17569
                     /locus_tag="ACHOSM_RS14430"
                     /old_locus_tag="pgaptmp_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="morphogenetic protein"
                     /protein_id="HGU4559369.1"
                     /translation="MLLPLFPLPSRPTELIQFRQPNIADAMRFNSITPEEQEQQTTAY
                     LKALLAEPAKYDPLTWTAQDRITALWWIFTGSRETPVETFTYTCKHCGKEHYYDCDMN
                     ALAEDIQVLEVEPFIDDIEVSVEGVPYQWRIVPLDGWAMEMLEMRRAALPPEDDAEFK
                     EAIVDLRFWEFAYQCELYNDVSGTREEQAERRYETIKRMAIDTEFMKLAAHIRLAHEK
                     LEHGLPCYIDKGEMRLRLPPHKCPNQDTKESTEGAYTRLWVPFRATDFIPQVGIEKLS
                     DLSVQPGFVWGYTDSGR"
     gene            17651..21043
                     /locus_tag="ACHOSM_RS14435"
                     /old_locus_tag="pgaptmp_002887"
     CDS             17651..21043
                     /locus_tag="ACHOSM_RS14435"
                     /old_locus_tag="pgaptmp_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HGU4559370.1"
                     /translation="MERKNANIDDVIRTVETASAKELEELAGIREAVEDLKGGRVATV
                     DPVSRSVSALNRTIENSRPDFVANAPSVDPIVEAMKRLNLGDVSRVVQEDVALQEPQA
                     KSTTRKGKRRRKKAITEDVKAQRTEAAEHAREMFGQKGGAQKSQNQRDARGRFIGKSG
                     SKAAAEDARAERAEKARRKEDDERLNAESGLLKKLSKVAEGIGNPSETRAVDALGYAV
                     AGPLWAAGKELGGISKEVGGSLNGARKSIADVIRGNDDNSRRKGFFRRKSQNSADVVQ
                     VNTQKRTVQELQEQTSEIKEGNDKILSALDQIAKNTGKKKGGLLSKLFSLLGKGAGGV
                     ASLLMGRGMLKKAGALAFGALGAKKLVGMLRGGGKKALAHEGGDLAARAAGKLGLKAV
                     GKGALRAIPLVGTVAGGIYDAVTGWNDTEAQRRAFGLKSGQDPSFQQKAAYTLANVLD
                     MGGLVSGISSAIGDVLKSLGFEDIGNMLQSFSTESIAQAIDSGITNLETYISNLGDTI
                     STKFDDYTAKIGDAVSAWFSDTSNKLLEKLDAIKDFFTVDNLKQVFSDAIDSAIDFIK
                     NPGKHIKEAAGNIWDGVKNLPGKALDAAVDAVKNTPAAMIVSKIPNPIGEANAKEITP
                     ELKAPVNSHQETSDSKTESDAKQTNIATRVINAALDTAKDSNKTVKETANQIINANAV
                     ETGNSAVRKIDSAIGQNSSSSSSRNTTGTRNDIQKAADTYNNGNLDVKVGSLGAEGKA
                     NLDKLAPYFAELENKYGLPEGTLYAIAATESGGNPYAKSQTGALGMFQFTGIAREETG
                     LAEGESFDPVKSAEAAALLMSKYLKQANGDLNEAITAYNAGFGTINKWKKGTGDLSKE
                     NREYAIKVNTHRARYLGGEIYTPGAGAQGGAQYGVRGPLPDNAVIDQSTGLAFTPGDS
                     PFEKGGLVDKIGNAVGVNDLVNKFMNGRGMRREVVQGTLEERARGKGTATAAGNVYVD
                     TPMPVEEARPVASNSSYFDQLGAQMGIDGLFDKLRNSPGMRKNNAPEPASTSQVTTAA
                     NDLQQPTGRMQIDGQVISDLGGSGAKPTMQLADNTVSLDGETKRLFAQMTSLLARIEE
                     HTKDSAKGQGTVVKVSTPQPGVMRTVPLSIDDPLMNDYARVD"
     gene            21043..21399
                     /locus_tag="ACHOSM_RS14440"
                     /old_locus_tag="pgaptmp_002888"
     CDS             21043..21399
                     /locus_tag="ACHOSM_RS14440"
                     /old_locus_tag="pgaptmp_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559371.1"
                     /translation="MANNNEIDPLLTLELSGVKTYESQEEAWGARLYEWLNTYQGEVY
                     GDPSWGNVLPQFKHEPTNLSHVQIAVEAMLLQKLTVDLPDIPISGLSVAEGDAFDKLK
                     ISIRIRDITITQDVVL"
     gene            21396..22829
                     /locus_tag="ACHOSM_RS14445"
                     /old_locus_tag="pgaptmp_002889"
     CDS             21396..22829
                     /locus_tag="ACHOSM_RS14445"
                     /old_locus_tag="pgaptmp_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin hydrolase"
                     /protein_id="HGU4559372.1"
                     /translation="MSKTTPTKDSIRAEFEELVEKDSFWSKFVGSQFVSMLTLFITQI
                     VYRCFQYADAALAEGFISTATRRSSILAAAETNSYVGTKPTPSSGMIEITATSEDAPA
                     VIPKNMPLISDDQYPYMTMDVCRLVDGTGTVEVAQLEIQEVTYTVTAAKEFLEVVLSK
                     ALTAVCYKLEVFVTTDGKTTQWSSSTMFRLAGSKSQVYVEFYKPSEQLGVRFGDGLIG
                     QIPPEGSTITLKVWCTNGDITLVAGQNLTPVDSAANLANLISVKTTTPITAGTDAETT
                     EITRNRAQYYLAYDDQVVWGGDYTYFLVRNIPGLSWVKAWGEGQQEKLDGAYNVLNIN
                     KIFISGWHPNKSQSELEEMILAAFKKVPNELNKKFSYKEVRKLPFKITITGRISASLT
                     IENVTDELKSALETKFGRDSTFFDPNRVGKYILIKKKDVWAFIETLGYFRDFYLEFVE
                     WNESNGFYDFVYLDTENSTFNISYEEE"
     gene            22829..23665
                     /locus_tag="ACHOSM_RS14450"
                     /old_locus_tag="pgaptmp_002890"
     CDS             22829..23665
                     /locus_tag="ACHOSM_RS14450"
                     /old_locus_tag="pgaptmp_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4559373.1"
                     /translation="MQRSWFNNRLTSAKQKSLLYKSLADLIQSMMDTFVDPWLERITN
                     RKSIFSMSKEDLETRTNELGQFFTIRTSNSSSVPMLLQQRLDEIHFKGTERPINQTIY
                     REFNGISVLWDPIYAPVDLERHPYGTVLIPESTLETTGGTFGEMFLTSRGMISIPIND
                     LARTMGITGTIDQSAITEEILRKFNQFVKPLLPLHIVFDGLTLYLSVVVNEQADMITL
                     NEISDTEKAFCWFETSDTTSLTGVTSISAPITATPGGTIVKATPTFDRTRADDLLLDS
                     DA"
     gene            23744..24178
                     /locus_tag="ACHOSM_RS14455"
                     /old_locus_tag="pgaptmp_002891"
     CDS             23744..24178
                     /locus_tag="ACHOSM_RS14455"
                     /old_locus_tag="pgaptmp_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4559374.1"
                     /translation="MSDVSTNLYKSQLLDYYYQRRAESSINKGSRFLISKAVFGTSSL
                     VTKKGDGTYEIGELPKAFDLAELTSQFCTINLVPTYSGGIITVRMDLDQSQLQEGKNY
                     PFNTLVVLDNENKPIAIICVQEDSLYVGKTYTAVMAINTTTA"
     gene            24190..27039
                     /locus_tag="ACHOSM_RS14460"
                     /old_locus_tag="pgaptmp_002892"
     CDS             24190..27039
                     /locus_tag="ACHOSM_RS14460"
                     /old_locus_tag="pgaptmp_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HGU4559375.1"
                     /translation="MNDVTVVTSVTYPSPESLALVADVQYHEPYLSAALNRKFRGIVD
                     PGFYAGFLPKPGGGMNLLITSVDGDKTAGAASVDIGEFYQVTIQHRKDISLALSAGKK
                     YAIVLKGRYLLGEDTYQVNTASHIHAAEFVTRTYTDSYQLGDGELLVCTVNIPAGVSA
                     ITQEMIDTSERINRTIGIDISDSVTSSRSDVAASLLAVKKAYDLAKSKYTAQDASTTQ
                     KGLVQLSSETNSDSETMAATPKAVKSVKDLADTKAPIESPSLTGTPTAPTAAQGTNST
                     QIANTAFVKAAITALINGAPGTLDTLKEIAAAINNDPNFSTTINNALALKAPLASPAL
                     TGIPTAPTAEQGTNNTQIATTAYVRAAISALVGSSPEALDTLNELAAALGNDPNFATT
                     MTNALAGKQPLDATLTALAALATGANKLPYFTGKDTVAQTDLTSVGRDILAKTSTLAV
                     IQYLGLRELGTSGEKIPLLSTANTWSARQTFNGGITGALAGNADTATKLKTARNINGV
                     MFDGSADININTLVSRGRVTALEANAQGTSGIQLYEAYNNGYPSPYGNVLHLKGATAA
                     GEGELFIGWSGTSGAHAPVHIRSRRDTDSANWSEWAQVYTSKDSIPGVNAKGNQDTSG
                     NAATATKLQTACTINGVSFDGSTDITLTAAHVAAFARRATDTYADADGGVPWNAESGA
                     YNVTRSGDSYILVNFYTGVGSCQTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSK
                     NLPQESYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIK
                     GKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKTTSSFDYGTKTTNS
                     AGNHSHNIPVGHTGAGNGVSAGYNAALGTGTTSSAGGHAHNVYIGAHNHTIGIGAHAH
                     SVIIGPHGHTITVNATGNEENTVKNIAFNYIVRLA"
     gene            27039..27617
                     /locus_tag="ACHOSM_RS14465"
                     /old_locus_tag="pgaptmp_002893"
     CDS             27039..27617
                     /locus_tag="ACHOSM_RS14465"
                     /old_locus_tag="pgaptmp_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4559376.1"
                     /translation="MTFRMSEQSRTIKIYNLLAGTNEFIGKGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWNLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIRKAEETKNSLMQMASEHIAPLQDAVDLDIATEEE
                     ASLLTAWKTYRVLLNRVDTAVAADVEWPVAPQ"
     gene            complement(27661..28233)
                     /locus_tag="ACHOSM_RS14470"
                     /old_locus_tag="pgaptmp_002894"
     CDS             complement(27661..28233)
                     /locus_tag="ACHOSM_RS14470"
                     /old_locus_tag="pgaptmp_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGU4559377.1"
                     /translation="MLCYLIAIHFVIFGNSKALLSYWMAEVIRREKMDVWRLLREKRQ
                     RNRNFLFWWRLANEMHINGNKLHKKAAKKLNSKIINKFGCEIGLGANIGKGLTIPHHA
                     GIVVHFAVDAGENLVLRQNTTIGQIDGDIPGSRVKIGSNVDIGANCCIIGLSRKIGDN
                     VKIGAMSFINKDIPSNCTYITKKSGVVLYK"
BASE COUNT         7624 a         6402 c         6957 g         7256 t
ORIGIN      
        1 agaaatcgta atatcaacac tcatgacttt actttggcag gaagccacta aggacaaaca
       61 acatgtttgc gattaaacaa gaaataaaca acgctacttc tctgttcgaa attgagagta
      121 ttaccgttgg atatcctggt tcagatcaat ttaaacaggc gttcgagatt gctgaagagc
      181 tgggtatcaa aacgcctgat gcgattgaat atatccctgt tgcgtacgaa gatgaggaga
      241 tgacaaaggc aatcggggaa gagcaaactt tgagtacaga gcgtaaggat gtacagcggg
      301 atgactgtat cgccgtgatt tgctctgggg ttgcttctga aatgttcccc gacctccctg
      361 ccatgggagg tgttggttat caattcctct acaaaggtga tacgttacgt atctatacaa
      421 gtagtttgtt gatcgaagaa gtaggtacgg aaaacgacaa ctaattcgct tcaacccttc
      481 acagaaatcg gtcctcagtg gccggttttt cacttatcca cattatccac tggatagatc
      541 caataatcag gtccatacag atcccaatta gatccatata gatccctgat cgtcgtaggc
      601 cgcgccccgt ctggcttaga agcctttcgc gatgtgtgct ggagggaaaa tgatgtgcgc
      661 tggagggaaa aaaatgtgtg ctgacgggtt gctaatgtgt gctggcggga tataggatgt
      721 gtgctgacgg gaaagcctgg gtagttatca ccgcttataa aaactatcca cataattcgg
      781 aaaaagtaat atgaatcaat catttatctc cgatattctt tacgcagaca ttgaaagtaa
      841 ggcaaaagaa ctaacagtta attcaaacaa cactgtgcag cctgtagcgt tgatgcgctt
      901 gggggtattc gtgccgaagc catcaaagag caaaggagaa agtaaagaga ttgatgctac
      961 caaagcgttt tcccagctgg agatagctaa agccgagggt tacgatgata ttaaaatcac
     1021 cggtcctcga ctcgatatgg atactgattt caaaacgtgg atcggtgtca tctacgcgtt
     1081 cagcaaatac ggcttgtcct caaacaccat ccagttatcg tttcaggaat tcgctaaagc
     1141 ctgtggtttc ccctcaaaac gtctggatgc gaaactgcgt ttaaccattc atgaatcact
     1201 tggacgcttg cgtaacaagg gtatcgcttt taagcgcgga aaagatgcta aaggcggcta
     1261 tcagactggt ctgctgaagg tcgggcgttt tgatgctgac cttgatctga tagagctgga
     1321 ggctgattcg aagctgtggg agctgttcca gcttgattat cgcgttctgt tgcaacacca
     1381 cgccttgcgt gcccttccca agaaagaagc tgcacaagcc atttacacct tcatcgaaag
     1441 ccttccgcag aacccgttgc cactatcgtt cgcgcgaatc cgtgagcgcc tggctttgca
     1501 gtcagctgtt ggcgagcaaa accgtatcat taagaaagcg atagaacagc ttaaaacaat
     1561 cggctatctc gactgttcaa ttgagaagaa aggccgggaa agttttgtaa tcgtccattc
     1621 tcgcaatcca aagctgaaac tccccgaata agtgtgtgct ggagggaaac cgcattaaaa
     1681 agatgtgtgc tgccgggaag gcttgtccaa tttcctgttt ttgatgtgcg ctggaggggg
     1741 acgcccctca gtttgcccag tctttccctc cagcacacat ctgtccatcc gcttttccct
     1801 ccagtgcaca tgtaattctc tgtctttccc tccagcacac atatttgata ccagcgatcc
     1861 ctccacagca cataattcaa tgcgacttcc ctctatcgca catcttagac ttttattctc
     1921 cctccagcac acatcgaagc tgccgggcaa gccgttctca ccagttgata gagagtgaag
     1981 cctggctgct cgttgaagca ggaaatcacc aaaatgattc aggctacaac ctgaacatag
     2041 aagaaatccg cgtcctttat gcgtggagga tgccaaagca tgttgtgaca cacttggcaa
     2101 aggagtaaac atgcagagaa tgctatgtac aagcatctac gcatacatta ttattttatg
     2161 cagcattttt aattaaattc aaaaatacag cataaaggat gactttcgat gagtgattcc
     2221 agccagcttc acaaggttgc tcaaagagca aacagaatgc tcaatgttct gactgaacaa
     2281 gtacagttgc aaaaggatga gctacacgcg aacgagtttt accaggtcta tgcgaaagcg
     2341 gcactggcaa aattgcctct actgactcga gcgaacgttg actatgccgt aagtgaaatg
     2401 gaagaaaagg gttatgtttt cgataaacgc cctgctggct cttcaatgaa atatgcgatg
     2461 tcaattcaga acatcattga catatatgaa catcgcggag tgccaaaata ccgggatcgc
     2521 tacagcgaag cgtatgtgat tttcatctcc aatcttaaag gcggtgtgtc aaaaactgta
     2581 tcgacggttt ctctggcgca tgcaatgcgt gcccaccctc atcttctgat ggaagattta
     2641 aggattctgg ttattgacct tgatccgcaa tcttcagcaa cgatgttttt aagccataaa
     2701 cactctattg gtatcgtaaa cgcaacatct gcacaggcta tgttgcagaa tgtaagccgt
     2761 gaagagctgt tagaggagtt tattgttcct tctgttgtac ctggggttga cgttatgcct
     2821 gcgtcgattg acgatgcctt tattgcatcc gattggagag agctgtgcaa tgagcatcta
     2881 ccgggtcaga acatccatgc tgtcctgaaa gaaaatgtga ttgataagct gaagagcgat
     2941 tatgacttta tcctcgttga tagtggtcct caccttgacg ccttcctgaa aaatgctttg
     3001 gcctcggcca atatactgtt tacacctctg ccgccagcaa ctgtcgattt ccactcatcg
     3061 cttaaatacg ttgcccgcct tcctgagttg gtgaaactca tttcggatga aggctgcgag
     3121 tgccagcttg cgactaacat tggttttatg tccaagttga gtaacaaggc agatcataag
     3181 tattgccata gcctggctaa agaagtgttc ggtggggata tgctagatgt cgtcctccca
     3241 cgccttgacg gttttgaacg ttgcggcgag tcttttgaca ctgttatttc agctaacccg
     3301 gcaacgtatg ttggtagtgc tgatgcattg aagaacgcgc gaattgccgc ggaagatttt
     3361 gctaaagcag tttttgaccg tattgaattt atcagatcta actgaggagt aagaaacccc
     3421 catgtcaaag aaaaacagac caacaattgg gcgaaccctt aatccttcaa tattaagcgg
     3481 atttgatagt tcttcagcct ctggcgatcg agtcgagcag gtattcaagt tatcaactgg
     3541 tcgccaggcc acatttatcg aggagataat tcctccgaac caggtagaaa gcgatacctt
     3601 tgttgatcag cataacaacg ggcgtgatca ggcatctctt acgccaaaat cattaaaaag
     3661 tatccgaagc actattaagc atcagcaatt ttaccctgca ataggtgtta gacgggctac
     3721 agggaaaatt gaaattttgg atggttcccg gcgtcgagct tctgccatct tagagaacgt
     3781 agggttgcgg gttttagtca cggaccagga gatcagcgtt caggaagcgc aaaatttagc
     3841 gaaagacgtt cagacagcat tgcagcacag cattcgagaa ataggtctgc gtttgatgcg
     3901 aatgaaaaat gatgggatga gtcagaagga tattgcagcc aaagaagggc tgtctcaggc
     3961 gaaggtcacg cgtgctctcc aggcagcgag tgctccggaa gaattagtcg cccttttccc
     4021 tgtgcagtcg gaattaacct tttcggacta caaaacgctt tgtgctgttg gcgacgaaat
     4081 ggggaacaag aatttagagt ttgatcagct tattcaaaac atatccccgg aaataaacga
     4141 catcttatcc attgaagaaa tggccgaaga tgaagttaaa aataaaatcc tgcgcttgat
     4201 aacaaaggaa gcctcactac tcacggataa aggttctaaa gataagtccg tagttactga
     4261 attatggaaa tttgaggaca aggatcgctt tgcaaggaag cgcgtgaaag gccgtgcatt
     4321 ttcttatgag tttaatcgac tttcaaaaga gctacaggaa gaactcgaca ggatgattgg
     4381 gcatatcctt agaaagagcc tcgataaaaa gccgaagcct taaactttcg ccattcaaat
     4441 ttcactatta acctactgtt tttaaagtaa atgcctctaa aatttcaagg tgaaatcgcc
     4501 acgatttcac cttggatttt accttcctcc cctactcccg aaaaaaataa aaaaattact
     4561 tgtcacgaga aagtcaacaa gtgactttca ataaaatctc ttccgaaaag ggattcacac
     4621 aagtgccttg tgtttaagga agagtaaatt gagtaactta cgcgaatacc agaatcgtat
     4681 tgcagatatc gcaaaacgct ctaaagctgt gcttggctgg gcaagcactg cgcagttcgg
     4741 tactgataac caattcatta aagatgatgc cgcgcgtgcc gcatctatcc ttgaagctgc
     4801 acgtaaagac ccggtttttg cgggtatctc tgataatgcc accgctcaaa tcgctacagc
     4861 gtgggcaagt gcactggctg actacgccgc agcacataaa tctatgccgc gtccggaaat
     4921 tctggcctcc tgccaccaga cgctggaaaa ctgcctgatt gagtccaccc gcaatagcat
     4981 ggatgccact aataaagcga tgttggaatc cgtcgcagca gagatgatga gcgtttctga
     5041 cggtgttatg cgtctgcctt tattcctcgc gatgatcctg cctgttcagt tgggggcagc
     5101 taccgctgat gcgtgtacct tcattccggt tacgcgtgac cagtccgaca tctatgaagt
     5161 ctttaacgtg gcaggttcct cttttggttc ttatgctgct ggtgatgttc tggacatgca
     5221 atccgtcggt gtgtacagcc agttacgtcg ccgctatgtg ctggtggcaa gctccgatgg
     5281 caccagcaaa accgcaacct tcaagatgga agacttcgaa ggccagaatg taccaatccg
     5341 aaaaggtcgc actaacatct acgttaaccg tattaagtct gttgttgata acggttccgg
     5401 cagcctactt cactcgttta ctaatgctgc tggtgagcaa atcactgtta cctgctctct
     5461 gaactacaac attggtcaga ttgccctgtc gttctccaaa gcgccggata aaggcactga
     5521 gatcgcaatt gagacggaaa tcaatattga agccgctcct gagctgatcc cgctgatcaa
     5581 ccacgaaatg aagaaataca ccctgttccc aagtcagttc gttatcgcgg ctgagcacac
     5641 ggtacaggcg gcgtatgaag cacagcgtga atttggtctg gacctgggtt ccctacagtt
     5701 ccgcaccctg aaggaatacc tgtctcatga acaggatatg ctgcgtcttc gcatcatgat
     5761 ctggcgcact cttgcgaccg acacctttga catcgctctg ccggttaacc agtcctttga
     5821 tgtatgggca accatcattc gtggcaaatt ccagactgta tatcgcgaca ttattgagcg
     5881 ggttaaatct tctggtgcga tggggatgtt tgctggtgct gatgcagcat ctttcttcaa
     5941 acagttgccg aaggatttct tccagccagc cgaagactat atccagactc cgtatgttca
     6001 ctacatcggt accctgttcg gtaacgtgaa agtgtacgaa gtacctgctg gtatttgtaa
     6061 gaacttaacg acagagaaca ttcagttcag ctcgatggat gtgctgtgct acgtccgtga
     6121 tgaaaatccg ggtaaagcag gcttcgtgac tggtgatgct gtcccggcca tcccgttcca
     6181 gcatccgacc actccggcgc tggtcaaccg taccacgctg tggggttctg ctatcaacga
     6241 tatgcaccca cgcaacggcg ctgattactt cactcgtgta acgctgacaa tggccaaaaa
     6301 aggcgggctt aacttcataa gcggcgacac gattgatgcc ggtgactctg agtaatcagg
     6361 ggaagttctc cgtttaacat agcgcccccg tgcggggcgc ataacaggga aagttatgtc
     6421 tcaatattca attcaacagt cattaggtaa tgcatccggc gtcgcggtta gcccgatcaa
     6481 tgccgatgcg acgttatcta ccggtgttgc attaaatagc agcttgtggg ctggtattgg
     6541 cgtatttgcg cgtggcaagc cgtttactgt tcttgcggtt actgagtcca attacgaaga
     6601 tgttctcggc gaaccgctga agccgtcttc cggctcacag ttcgaaccaa ttcgccatgt
     6661 atacgaagct attcagcaaa cgtcaggtta tgttgttcgt gctgttccgg atgatgcgaa
     6721 gttcccgatt attatgttcg atgaatcagg cgaaccggct tacagtgcgt tgccatacgg
     6781 ttctgaaatt gaacttgata gtggcgaagc ctttgctatc tacgttgatg atggtgatcc
     6841 gtgtatttca cctacccgtg agttaaccat cgaaacggca acagcggaca gcgcgggtaa
     6901 tgaacgcttc ctcttaaaac tgacccagac gacttcgctc ggcgtggtaa cgaccctgga
     6961 gacacacact gtgtctttgg cggaagaagc gaaagatgac atgggccgct tgtgttatct
     7021 gcctacggct ctggaagccc gttctaaata tctgcgcgcg gttgttaatg aagagctgat
     7081 ttcgacggcg aaagtaacaa ataaaaaatc gctggcgttc actggtggta ccaacggtga
     7141 tcagtcgaaa atctcaaccg ctgcgtacct gcgtgcggtt aaggtgctga acaatgcgcc
     7201 gtacatgtac accgctgttc tcggcctggg ttgctatgac aatgcggcga tcaccgcgtt
     7261 aggtaatatc tgttctgatc gcctgattga tggcttcttt gatgtcaaac cgacattgac
     7321 gtatacggaa gcgctctctg ctgttgaaga taccggttta cttggtaccg attatgtaag
     7381 ctgtgctgtc tatcacttcc cgttctcctg caaagacaaa tggacccaat cccgtgtggt
     7441 cttcggtctg tctggcgcgg cgtatgcggc gaaagctcgt ggcgtcaaga aaaactctga
     7501 tgtcggcggt tggcattact caccggctgg tgaagaacgt gccgtcattg ctcgtgcgtc
     7561 aattcaaccg ctgtatccgg aagatacccc ggacgaagaa gcaatggtca agggccgtct
     7621 caataaagta tctgttggca cctctggcca gatgatcatc gacgatgctt taacttgctg
     7681 cacgcaggat aactatctgc acttccagca cgtcccatcc ctgatgaatg caatcagccg
     7741 tttctttgtc cagttagccc gacagatgaa gcatagcccg gacggtatta ctgcggctgg
     7801 cctgactaaa gggatgacca aacttttaga tcgctttgtc gcctccggcg ctctggtggc
     7861 tcctcgtgat cctgatgctg acggtacaga accgtatgtg ctgaaagtta cgcaggcgga
     7921 attcgataaa tgggaagtag tctgggcctg ctgcccgact ggcgtagccc gtcgtatcca
     7981 gggcgtaccg ctgcttatta agtaagggaa tacaatgagc aaaaactttt ttcaatccgg
     8041 ggcatttttg gggaatggac tgtctcgttt cgctttgaac tctgatcctg tgcagctgat
     8101 ggagtctgcc cgagcaagcg ccgaaccgcc aacagatccg gttattaata ataatccgga
     8161 accggcggca cagactaacg ataacgttcc atctgccccg gatcctgagc aaatcctgga
     8221 agggaaagac ggtaaagaat ggaccgtcga acaggcgcac cagatgattc tggaagctgc
     8281 aaatcgaagt gctatgcaga atgcgttgag tgatgcggcc gacgccgttt tcgcctgggc
     8341 tgatagcggt gatctgactt tcgactccct tgatggtttc gttcaggcta tcgctggtat
     8401 ctctgatgac gacgactccg aagttacaga agaacaggac gatgcctata acgaagcatg
     8461 ggcaaatgtt gctgacttcc tcgcagcatg cggtgtagat gatgacctga tcgaagcact
     8521 ggctgacgat gaagacgacg acgcagctgc tgatgttggt gcctctatcg ctggtttaga
     8581 tagcgacgac cgtgacgaac tggaagcggc gtttgttgtt gctggcactt ctgatgaaat
     8641 gctgactgaa gcatttaaga aggttgttcg taacggtgag atcaaactca tccgtaaacg
     8701 cctgcgtaaa aaacgtctga ctgcggctca aaaatcggcg ctgaaaaaag cgcgtcgaaa
     8761 agcccagacc ggcgcggcaa aacttgcccg caaaaagtca atgaaactgc gccgtaagcg
     8821 ccttggctaa aggaggaggc cggagaactc cggcctttaa cttgaatggc acctatacct
     8881 tatggggttt acagccaggc tgacggtgta tcaccatttc tgaaagttac tttaacgaac
     8941 tctcagtacc aggttaccgg atatatcagc cagggagcgg caatgaacat ggcccagaat
     9001 tgggaagcgc cgtttaccgg tatgtccatg ggatctgttt ctggtgctct gggtggtttt
     9061 gtgcaagtag gtactgaaac aacgtcggtt gcccgttgga atagcttaat ggtttgggaa
     9121 ggggggactc cgccgacgtt cacgctgcct gtaactttca ttgctttgaa caatccattc
     9181 attgaagttt caggcgctat cgccgcgttg acagccatga ttagcccgga actaaaagcg
     9241 gccaatgttg gtggtcgaat cccggagcgc gtgacgctaa acattggtcg ccggatcaac
     9301 atcaccgatg tcgccatcca ggacttaagt tttgatctcg atgcgccaag ggacagtaat
     9361 ggatatttcc tgaaaaacac cgtcaacctc cagttgaccg gttcttcgat atataacagc
     9421 tccgatattg ttcgggcgtt ccagtaaaag gattttatat ggggcacaat aacactaagg
     9481 gaaaccgtaa atttattaag ggccgctata ctgccaacgc ggccaaaggc gaacgactgg
     9541 tatcttctga attccagctc acttttgcag gccatgaaga tatcagcgta ctggttcgca
     9601 cgtcgcaaat ccctgaaatg acccgcgagg atgtggagga ctatggtccg aatggtgtga
     9661 agttcaacca gcacggacca attcgaaact ctggggaaat ccaggtccag tgcgtggaga
     9721 ctatcgaagg cgatattctt cagttcatca aggatcgcat tgcggcgaag gactatgttg
     9781 atatcacgat ggctgctacc cctgaatcca aatcttccgg ggttaacgct gtgacaaaag
     9841 ctgctacaac aattgaaatg ttggactgca aaatctacag tgatgcaatc gactttagta
     9901 ccgaagatgt gactgccgct gtgcgcccgt cacttcgtat cgtctacaac tggattgagt
     9961 gggattaaga gtcatccctt gtattttaaa gctccttcgg gagcttttta atttggagag
    10021 gaaagggtac attgaggata cctgacacac gaagagtggc ggggatctct ccccgccagg
    10081 tctcttacct ttcagattcg taggctgtga agacagtgac ctccgtctgg ccggttcgga
    10141 ttcgtacctc gcagaggtct ttcttcgtta ccagtgccgt cacaatgacg gttaaacaga
    10201 tgacgatcag agcgattaat atcgctcttt gctgcttcat agcctgcttc tccttgacct
    10261 tttggtcggt aagaggctaa tctacgtatg ctaagcatag atattgcctc agattaatgt
    10321 taagcgtctt gcaggacaca taatgttatc tggggctttc ttctatctgc ttttcgggta
    10381 atgcctgaag cagatagcct caagcacccg caacgattgt atcaatgtct ggcttttttt
    10441 ctatagaaat cacctggaag ggtgaatatc cacatcagaa gaaatgttgc agcaaacatg
    10501 atccctaatg gccagaccgc gccaaagaaa atccattcta agatctcctc tgcttgttct
    10561 ttgcggtcga tatcgacaag catttttcgg ctgatcatgt atacacagaa gccaatacaa
    10621 acatatcctg caaaagcgat cgctaactgt aaaaaatcag attgcatctc cgacctcaaa
    10681 ctgaaaacgc caggtgactc cagattagag caatctatca ccctctgaat cctgccggta
    10741 taccccattg ttcgttatct ttatttttgg ctaaaaccgc attaagagct tcgtttaccg
    10801 tcatgcaatg cggcagatta tcgaagtttg ataccccgcc aatatcagga gaatgcttgt
    10861 tcttcaggta agcatatttc cgcgctgccg cctctacttt ctgcttgaac tcatgttttt
    10921 gagcgcgttt tttggataac cgcagattgt cagcctttgc ttttgcctca gcgatccagg
    10981 aagtcaaatt tttgagcctg gtcgttccgg caccgccgga aactgatctt tctgtttttt
    11041 taacttgtga cttcttattc tttattgcca cgtcatcctg acagggggag ggggtatcat
    11101 tttgacatgg gggtgtggat aaaaaattaa ataaagccaa tgtcttagcg agaacagctt
    11161 taaccttggt tgccgctgaa gagatcttta atttgctttc aatcagcgca tttttggctt
    11221 gttgtgcgaa ggccaaaaat gatggtgtaa accggtacag gttagcgcga cgttcacggt
    11281 gatcgccgat aacaatctct acagacagaa ttcctttgtt tacagcttca cggaatgcac
    11341 gaacgacggt tgattggcta taaccagttt ctgccgcgat caggcggtga ggcttgtgaa
    11401 tgaagtattc actggttgtt gccgcgagat ttgcacattg cgacaggata tgcccggcgc
    11461 tacgggatag accggagtgt gttacaaagc aggccaattc atagccagaa aaagtaaaat
    11521 cgctcatcgt tatacagctc aggaaagtga ctttagccag cattacaatg ctggtggttc
    11581 ttactacgtt tgttagcgcg ttgccgcgac aggtaccagc acaccagcat caagcaatcg
    11641 cttcataagc cactgctgac ctttgccggt tatacgagtc gtgaaagaaa tcctgcttcc
    11701 attgcttgta tcgatcacgg tttctttaag ggtgaaatac ccacgggata tgtattcttg
    11761 tttggggacg ttcctgcgtt caccggttgc gatcagaatt ccgttatcac gcaaccaggt
    11821 gaagagatag ttttggccca ggccgagcac tttggcatag ttgccgatta gaaccccgct
    11881 ggcggtagca acgcgttcgg cgaattcgac tttaggtgca tccatcagca ttttttgctc
    11941 cagccgttgc ttttgctctg ccaggtcggc agccaaacgg agagcttcag ggagactctg
    12001 cggaatagca ggttgtaatc ttccggctcg atagtcgata aatgtctggt ttaccttcag
    12061 ccgaaacgcg ggagaaatcc agcctgcgta ctccacagcg agcaattcat gggcaaaagt
    12121 gccgccgcca cggccttcga acgaaactat gcaattctgc atagtttctt tttcaagctc
    12181 ttcgatgagc tgttttgctg acagcgttct tagccattga gctggcgctt tatgggcacc
    12241 gagtccgctc gctctgtgta gagcattaag gttgtaacgg ccagcgcggt cggtcgtaat
    12301 ttcaacacca caaataacgg gcagagtggt tgaaggatcg acattttgat gaaggtttga
    12361 tatattcata tccgcattga atgtttgttg cattttttct ccaaatttgc atcaaccttc
    12421 aatcaccagc tcgaaatggt gattatttgc acttagaaaa cgaaatttat tagagcaaat
    12481 ttttctaact cgatccagat cgggttggtc gatctgctca gaaacctgcc agtttgctgg
    12541 caggtttttt tattttgtta acctattgct actggtttta acaaaccagc atcaagtagc
    12601 ttgcgagtta accactgctg gcctttaccc gttaattggg gcgtcagccg tatctggtag
    12661 ccattttcat catccagcac cacttctttc actgtgaaat acccggcgtt gatgtactgt
    12721 tggcgcggta cgtttttgcg cgcaccaaaa gccatgagaa tgccgttctg gcgcaaccat
    12781 gagaaaaggg cgttttgctt aagtccaacg acctttgcaa agttcccgat caggattcca
    12841 ttggccactg atacccggtc ggcaaaatcg actttagggg ctgcggccac cagctgttgt
    12901 tccaactgca ttttctgttc tgctaactcg gcagccaggc gtagggcttc tggtaatgtc
    12961 tgggggatcg atggggtagg ggagtttgcc tgctgtaatt cttccagttt gtcgatcagc
    13021 gaacggcgga ccgcttttga ctcgcgcgcg gcgactcgca gggcttgttt gaaagtcatg
    13081 gttatgacaa gcattgatgt tttgttcatt ttttccacta cactttttgt gtagtgctca
    13141 ccatcaagct catcgagtat tttttcgatg aatttgttgt tccgaacctc tggttcccca
    13201 cataacttac gcgcttcatt gaccatcttt aacagtgtca ggctgtcgat tgtgtctccg
    13261 gtgttgggga tgatattcac ggctggtgct ggcgtagctg aagcaacagg tgctggtttt
    13321 tcaacattta aattattacc ggtcattcta tgtgcctcct ttctcatttc tgctgccact
    13381 gttgcgtaac gtagacgtcc ttgttcaatc aaataatccc tgatctcggc tatcagtagc
    13441 ttgttgatca cagccttatc tgttcgggta taaaaacgtc tggttatcat gaaatagttg
    13501 gcaattgcgc cggggatctc ccgtgtcggc atacaggcag tatgcagggc gatcgcttcg
    13561 gttatgtcat tacgggtgac gagaggtttt ttcataaacc cccctgaacg tcggcagaga
    13621 aggggaggtt ccagtaacta agtgaattgc gcgagttagt tgaaaaacgg gcagtaaaaa
    13681 tgcaggggcc atcaggcaat tgagagcgtg cttcgtcttc tgttgctgcg ataacgaagt
    13741 gatagtgggg ttttttacag gaatagaaac gccagatgaa ttcttggcgt gcgcaaggat
    13801 tggcattaac catagttacg gcctcacaat caggtttaac aacctgctac ccgctgctaa
    13861 acaggtggca ggacgtgacg gggttagcag actggcgatt gtgaaaccag caggccgaag
    13921 cctccccatc acgccccacc ataatttggg cgtaacgcgg ttttacggac acaaaaatac
    13981 cgcaatatcg gatatctgcg gctgtccgca caatcattca ggctgctaaa cccggtcgca
    14041 gaatttgcta cgacggcgga actataagcc tgaacgatta aaaggtcaat atgatgcgaa
    14101 aagatagcat tcgcgactta aaaatacaaa tttattagag cattatatgc ttaataaata
    14161 cacaattgga tctaataacc tctttttttt aaaggcgaaa atatgtaccc taaatgagtt
    14221 ataaggcagg tgaggttata atgagaaaac tattactacc gttattattt atggctggga
    14281 ctgttaatgc agcatcaagc gtaaaggaga tttgtaccga ttatacgaaa taccttgggc
    14341 acgtttacgg ctttgctgtc agtcaagacg aatccatgcg caagaagtta ctgtcagata
    14401 tgaaacgcct taaactttct gaagcgatgg tgcagcaaga actgtataaa gtcgcaacca
    14461 acgaaaatgc taaatatcaa tattctcgcc tattaaatcc cgacgcaaac gagatcaatc
    14521 gaagctcttt cgattatatg gtaaaggcat gcgaaaccgc tcctgatttt gctatcccta
    14581 gctggggggt gctggtggcg agcaatgccg ttaataaaga agatgttgga agaaatggca
    14641 ttgactcaat cagaaatgcc ccaggaatgc gccatcaaaa cgtgcagggt acgcttgaag
    14701 agcgggccag ggggccgggt acaaactccc caatgggaaa cctctcacca gaggaattgc
    14761 aagagtataa ccaacggatg gagcagtatg agaaagcggc acgcgaacaa atggaacaac
    14821 aaaagaacgg ctcacttaat accttccagc aaggtttaaa agcgcttaat ttaccctatg
    14881 aatggtaaat atattatgga taaccaatta aaatattgat aatcgttaat atccaaagga
    14941 gataaattaa tgcgcatcaa ccgatttttt ctaattcttg ctttgcttac tccattttca
    15001 tcaatggcaa atgtaagcaa atggtcaacc ggcgagactc atggtgttcg ttcttatgct
    15061 gtttccagca aagataatta tacgcttaca tttgagtgcg atgttggatt taataatacg
    15121 gatcccaatc aagtaggaac acgactactc actctcatga aaacagaacc tggcggtgag
    15181 tcatttgatg ctaaaaaaga acaaataacg ctgaaagttg gtgatgatga atatcctatc
    15241 agttctatcg gttcctctgt gggtgatagt tactggtatg gtttttggtc agatacccct
    15301 gatatggaag ttaaaacatt cgatgcatac gtagacggaa aaaaaatcgc aacatttacg
    15361 ctacgtaagg ccgcagagct tttcaacgcg gcacctgaag atggctgcct gaagcgtgca
    15421 aaatgacctg tcacaaatga ctactcgtag aatcggttaa cacaccagat tctacgaggt
    15481 ttcaatgaca ccacgacaat tactcgaaga cgtcaaatcc cgcttcacac ctttgattgc
    15541 ggatgaacct gccttactgg aatccctgct aagaaaagca ttgggaacct accaggatag
    15601 ggcggggcac atcaagcgga tacgcatcac cgatcaggcc agtaaatcac ttgcttgccc
    15661 agttgatttt cttgcgctcg tatcggttac agatcacacc ggcgatcttg tctactccga
    15721 tgtttacgat gggaatatcg agcttgaaga tacccatcga gcggtatacc cactgaatgt
    15781 gtcatatctg gctaatttgc gtgatatgga tctggataat ggggatgtgc cacctgaaat
    15841 cattgggtta ctttctgact atctggaagt gctaatcgcg atacctaaca ctgatcgcct
    15901 gcgaagaata tctatcgcgg ggaaactcga tgccagtaat ttatccgacg agaacacgct
    15961 gtatcagcga aagctggatc tggaagagaa aatgagcgca acaagggcaa ttatcccggg
    16021 aattgtcctt ttctcatcca tgttgaagtg agggggctga tatggggctt aatgttgctt
    16081 cagtaaagtc ttatgtatct tcggcattaa cgacgacatt atttggctcc ggcgttggtg
    16141 agcgggaagt tggtaagctg acgtcaatca tcatgaacaa aatgctgttc gcgcaaggat
    16201 ggcagttctc tgtcgaagtt gatggtctgg agggggcaga cttctttgct aaagacatta
    16261 cctaccacga ttacagcatc gaatatgaaa cgattaaaat cggcggaggg aatatccttc
    16321 agccaacgga gcgttcgcct gggcagataa caatgatggt cagggatacc gttgatggcc
    16381 tcgttttgga ctggtttaag acggcaaaag gtcgggtgat caatccggac ggtaccggga
    16441 atataccgtc taaatatttg ctcaatgtgc gtatttatcg gttgctgtcc tccggtttaa
    16501 ccaaactgga aaatgagatg acggtattcc cggtcactac cggcgatgtc acctatgcgc
    16561 gggatcaggt tacggaattt aagtcattcc caatgacctt cgcattgcac agcacgttta
    16621 accaatcctc aagttcttta gcttcccttc tgggctttag tttttctctt tgaattaagg
    16681 agcaaggatg cttttacctc ttttcccgct accatcgcgg ccaactgaat tgatccagtt
    16741 ccgtcagcca aatattgctg atgcgatgcg tttcaactcg ataacaccgg aggaacaaga
    16801 acaacagaca acggcgtatt taaaagcctt gctggctgaa cccgcgaaat atgatcccct
    16861 gacatggacg gcgcaggacc ggattaccgc gttatggtgg atatttactg gctcccgtga
    16921 aacaccggtc gagacattca cctacacctg taaacattgc ggtaaagagc attattacga
    16981 ttgcgatatg aatgctctgg ctgaagatat ccaggtcctg gaagtggaac cgttcattga
    17041 cgatattgag gtgtctgtag agggcgtgcc ttatcaatgg cgtatcgtgc cgcttgatgg
    17101 ttgggcaatg gaaatgctgg agatgcgccg tgcagcattg ccacctgaag acgacgcgga
    17161 attcaaagaa gcgatcgttg atttgcgttt ttgggaattc gcttatcagt gtgaacttta
    17221 taacgatgtt agcggtactc gtgaagagca ggctgagcgt cgttatgaaa cgatcaaacg
    17281 gatggccatt gatactgaat ttatgaagct ggcggcacac atccggctgg ctcatgaaaa
    17341 gctcgaacat ggtttaccgt gctacatcga taaaggcgaa atgcgtcttc gtctcccgcc
    17401 gcacaaatgc ccaaaccagg atacaaagga gtccacagag ggtgcgtata cccgtctgtg
    17461 ggtgcccttt cgggctaccg acttcattcc acaggtgggg attgaaaagc tatcagacct
    17521 tagtgtccaa cctggttttg tatgggggta taccgattca ggacgctgaa aggctcactg
    17581 aatcctatgc gtttttcctg ttggagaaac tggaagaaaa acttaaaccg aagcggtagg
    17641 cgataagatc atggaaagaa aaaacgccaa cattgacgat gtgataagga cggttgaaac
    17701 cgccagcgca aaagagctgg aagagcttgc aggtattcgg gaagccgttg aagatttgaa
    17761 aggggggcga gttgctacag ttgatcctgt ctctcgcagt gtgtcggcat taaatcgcac
    17821 aatcgaaaat tcccggccag actttgtggc caatgcgcca tcagtggacc ctattgttga
    17881 ggcaatgaaa cggcttaatt taggggacgt ttctcgtgta gttcaggagg atgttgctct
    17941 acaggaaccg caggcaaaat caactacgcg aaagggtaaa agacgacgca agaaggctat
    18001 aacagaagat gtaaaggcgc aacggaccga agcagccgaa cacgctcgcg aaatgttcgg
    18061 tcaaaaaggc ggtgcgcaaa aaagccaaaa ccaacgcgat gcgcgtggtc gttttattgg
    18121 aaagtcaggg agtaaggccg cagcggaaga tgcccgtgct gaacgtgctg aaaaggccag
    18181 gcgcaaagag gatgatgagc gtctaaatgc tgaatcaggt ttattaaaaa aactgtcaaa
    18241 agtagctgaa ggcataggta acccttcaga gactcgtgcc gtcgatgcgt taggttatgc
    18301 cgttgctggt ccattgtggg cagcagggaa ggagcttggc gggatatcaa aagaagttgg
    18361 tggatcgctt aatggtgcca gaaagtctat tgccgatgtg attcgtggca atgacgataa
    18421 cagccgtaga aaaggttttt ttaggcgtaa atcgcaaaat agtgccgatg tcgttcaggt
    18481 taacacccaa aaacggacgg ttcaggaact tcaggagcag accagcgaaa ttaaagaggg
    18541 caatgacaag attctcagcg cccttgatca gatagccaaa aacaccggga aaaagaaggg
    18601 aggcttgctg tccaaactat ttagcctgtt agggaagggg gccggtggcg tcgcgtcgtt
    18661 gttaatgggg cgtggcatgc tgaaaaaagc tggagcactc gcttttggcg ctctgggggc
    18721 aaagaaactt gtaggaatgc tacgcggtgg tggcaagaag gctctcgccc atgaaggcgg
    18781 agatttggct gcccgggcag ctggtaaact ggggttaaag gcagttggta aaggggcgtt
    18841 acgcgcaatt cccctagtcg gcacagtggc tggaggtatt tatgatgcgg taaccggttg
    18901 gaatgataca gaagctcaac gtcgagcgtt tgggcttaaa tcaggacaag atccatcatt
    18961 ccagcaaaaa gccgcttata cgttagccaa tgttcttgat atggggggac tggtatctgg
    19021 tattagcagt gctattggtg atgttctcaa atcacttgga tttgaggata tcggtaatat
    19081 gttgcaatca ttttcgacgg aaagtattgc ccaggccatt gatagtggga ttaccaactt
    19141 agaaacatat atttctaacc ttggcgacac catttctacc aagttcgatg attacacagc
    19201 aaagattggt gatgctgttt cagcatggtt tagcgataca tctaataagc tgcttgaaaa
    19261 gctggatgct atcaaagact tctttactgt tgataacctg aaacaggttt tcagtgatgc
    19321 aattgatagt gcaattgatt tcattaagaa cccagggaaa catattaaag aggcggctgg
    19381 taatatttgg gatggggtta aaaatttacc cggtaaagca ttagatgcag cggttgatgc
    19441 cgttaaaaat actcctgcgg caatgattgt atcaaaaata cccaatccga tcggcgaggc
    19501 taatgcaaaa gaaatcactc cagagttaaa agctccggtt aatagccacc aggagacatc
    19561 tgattctaaa actgaatccg atgctaaaca gactaatatt gctacccgcg tgataaatgc
    19621 ggcactggac acggcgaaag atagcaataa aacagttaaa gaaactgcca atcagattat
    19681 caatgcaaat gccgtagaaa cgggcaatag cgcggtgcgg aaaattgatt cagctattgg
    19741 acaaaatagc tcgtcatcat cgtcgcgtaa taccaccggc accaggaatg acattcagaa
    19801 agctgctgat acctacaata atggcaactt agatgtaaaa gtcggaagcc ttggcgctga
    19861 aggtaaggca aatctcgata agttagctcc gtattttgct gaactagaga ataaatatgg
    19921 tcttcctgaa ggcactcttt acgcgattgc tgcaactgaa tctggtggta atccatacgc
    19981 aaaatcccaa actggtgctc taggaatgtt tcagttcacg ggcattgctc gtgaagagac
    20041 tggcttagct gaaggtgaat cgtttgatcc tgtgaaatcg gcagaagctg cggctcttct
    20101 catgagcaag tatctgaagc aagccaatgg agacttaaac gaggccatca ctgcatataa
    20161 cgctgggttt ggcactatta ataagtggaa aaaaggcacc ggtgacttat cgaaggaaaa
    20221 ccgtgagtac gcgatcaagg tcaatactca tcgtgctcgc tatttaggtg gtgaaatcta
    20281 tacacctggg gcaggagcac agggtggggc gcaatatgga gtgaggggac cactgcctga
    20341 taacgccgtt atcgatcagt ctactggtct ggcgtttacc cctggtgata gcccgtttga
    20401 gaaaggcggt ctggtagaca aaatcggcaa tgctgttggc gttaacgatc tggtcaacaa
    20461 attcatgaat ggccggggta tgcgtcggga agtcgttcag ggaacgctcg aagaacgtgc
    20521 acgagggaag gggaccgcaa cagcagctgg caatgtgtat gttgatactc cgatgccagt
    20581 tgaagaggcg cgtccggtgg ccagcaactc aagttacttt gaccaactcg gcgcacaaat
    20641 ggggattgat ggactattcg ataaactccg caactcgccg gggatgcgga aaaataatgc
    20701 gcctgaacca gcctccacgt cccaggtgac gactgccgcc aacgatttgc agcaaccaac
    20761 cggtcgtatg cagatagacg gacaggttat tagtgacctt ggcggctccg gtgccaagcc
    20821 gacaatgcag ttggctgata ataccgtttc acttgatggt gaaacgaagc ggctgtttgc
    20881 gcagatgacc tcattgcttg ccaggattga agagcacacc aaagactcgg cgaaaggcca
    20941 gggaactgtc gtaaaggtca gcacgcctca gccgggcgtt atgcgcacgg taccactgtc
    21001 aattgatgat ccgttgatga atgactacgc gagagttgat tgatggccaa caataacgaa
    21061 attgatcctt tgctgacgct ggagttatcc ggcgtaaaaa cgtatgagtc ccaggaggag
    21121 gcctggggcg ctcgtttata tgagtggcta aacacttatc agggtgaggt atacggagat
    21181 ccgtcatggg gcaatgtttt accgcagttt aaacacgaac cgaccaactt gtcgcatgtt
    21241 caaattgcgg ttgaggcaat gcttttgcaa aaactgacgg tagatttacc cgacataccg
    21301 atttctggct tgtcagtagc cgagggagat gcttttgata agttgaaaat atccattcgt
    21361 atcagggata taactatcac acaggacgtg gtgctatgag taaaacaaca cctactaaag
    21421 acagtattcg tgcagagttt gaagagcttg tcgagaaaga ttcattctgg tcgaagtttg
    21481 tcggctctca atttgtctcg atgctgacat tgtttattac ccagattgtc tacaggtgct
    21541 ttcagtatgc cgatgcggcg ctggctgaag gctttatatc gaccgcgacg cggcgttctt
    21601 ctatcctggc agcggcagaa acgaatagtt acgttggtac caagccaaca ccgtcatcgg
    21661 ggatgattga gatcaccgcc acaagtgaag atgccccagc ggtaatcccc aaaaacatgc
    21721 ctttaatatc tgacgaccag tatccttaca tgactatgga tgtatgcagg ttggttgacg
    21781 gcaccggtac ggtagaagtg gcacagttgg aaatccagga ggtgacatat accgttacgg
    21841 cagccaaaga atttctggaa gtcgtgttat caaaggctct cactgctgtc tgctataagc
    21901 tggaagtatt cgtgacgacc gatggtaaga ccacgcagtg gtcttccagc actatgttcc
    21961 ggttagccgg tagtaaaagc caggtctacg ttgagtttta taaaccatcc gagcagttgg
    22021 gggttcgatt cggtgatggg ctaattgggc aaataccgcc agaaggctcg accattacgc
    22081 ttaaggtatg gtgcaccaac ggcgatataa ccctggttgc tggccaaaac ctgacgcctg
    22141 tcgattctgc ggctaattta gctaatttga tttcagttaa gacaacgaca cctataaccg
    22201 caggtaccga tgccgaaaca acggagatca cacgtaaccg tgcacaatat taccttgcct
    22261 atgatgatca ggtcgtatgg ggcggggact atacgtattt tctggtgcgt aacatcccgg
    22321 gactgtcctg ggtaaaggca tggggcgaag gccagcaaga gaaattagat ggtgcttata
    22381 atgttctgaa tatcaataag atatttattt cagggtggca tccaaataaa agccagtcag
    22441 agcttgaaga aatgatcctg gctgccttta agaaggtgcc gaatgagttg aacaagaaat
    22501 tctcgtataa agaggtcaga aaactaccat ttaagataac catcaccgga cggatatcgg
    22561 caagcctgac cattgagaat gtgaccgatg agctgaagtc ggcgctggaa acaaaatttg
    22621 ggcgcgactc aactttcttt gatccgaacc gcgtcgggaa gtacatcctg attaagaaaa
    22681 aagacgtttg ggcgtttatc gaaacgctgg gttatttccg cgacttttat ctggaatttg
    22741 tcgagtggaa tgagtccaac ggcttttacg atttcgttta tctggataca gaaaactcca
    22801 cctttaatat ttcgtatgag gaggagtgat gcagcgttcc tggtttaata accggcttac
    22861 atcagctaag caaaagtcat tgctctataa atcattggct gatttgattc agtcaatgat
    22921 ggacaccttt gttgacccat ggttggagcg aattaccaac cggaagtcta ttttctccat
    22981 gagcaaggag gatctggaga ccaggacaaa tgaacttggc cagttcttta ctatcagaac
    23041 ctcgaactca tcttccgttc cgatgttgtt acaacagcgg cttgatgaga tccactttaa
    23101 gggaactgaa cgtcctataa accagacaat ttaccgcgaa tttaacggta tttctgtttt
    23161 atgggatccg atatatgcac cggtggacct tgagcgtcat ccctatggca cagttctaat
    23221 accagaaagc acattggaga ctaccggcgg cacattcggc gagatgtttc tgacttccag
    23281 agggatgatc agtattccca taaacgacct ggcccggaca atgggtatta ctggcacgat
    23341 agatcagtcc gcaattacag aagaaattct cagaaagttt aatcagttcg taaagcctct
    23401 actgccactg catatagtgt ttgatgggct tacgctctat ttgtcggttg ttgtaaatga
    23461 acaggccgac atgatcactt tgaatgagat ttctgatacc gaaaaagcat tctgctggtt
    23521 tgaaacttcg gatacaactt cgcttactgg agttacgtcg attagcgccc cgatcaccgc
    23581 aacgcctggt ggcactattg tgaaagcgac acctacgttt gatcgcaccc gcgcagatga
    23641 tttgttgctg gatagcgacg cctgacaatc accccgtccg cagggcgggg tgacaagtta
    23701 cttctcttac aatgaggctt cacaacattg attagggaaa atcatgtctg acgtctcaac
    23761 aaacctctat aagagtcagt tgttggacta ttactatcag cggcgcgctg aatcgtccat
    23821 taacaaaggc tctcgatttt taatcagcaa ggccgttttt ggtaccagtt ctctggttac
    23881 taagaaagga gatggcactt atgagattgg agaactgcca aaggctttcg atctggcaga
    23941 actgaccagc caattttgta ccatcaacct cgtgccaacc tactcaggtg ggataattac
    24001 tgtccgaatg gaccttgatc aaagccagtt gcaggaaggg aaaaactacc cattcaacac
    24061 tctggttgtt ctggataacg agaacaagcc aatcgccatt atttgtgtcc aggaggactc
    24121 gctgtatgtg ggcaaaacat ataccgcagt tatggccata aacacgacta cagcataagg
    24181 atatgcttga tgaatgacgt tacagttgtt acatcggtta cttacccatc acccgagtcg
    24241 ttggctctgg ttgctgatgt gcaataccac gaaccatatc tgtcagccgc gctaaaccga
    24301 aaattcaggg ggattgttga cccgggattt tatgccggtt tcttacctaa gcctggcggt
    24361 gggatgaacc tgttaatcac ctctgtggat ggtgataaaa ctgctggcgc agcgtcagtg
    24421 gatattggtg aattctacca ggtaactatt cagcatcgta aggatatttc tcttgcactt
    24481 agcgcaggta agaaatatgc aattgtgctg aagggaagat acctccttgg agaggatacc
    24541 tatcaggtta ataccgcgtc acatattcat gcggctgaat ttgttaccag aacctatacc
    24601 gattcatatc agttaggaga tggggagctg cttgtttgta cggtgaatat ccctgctgga
    24661 gtatcagcca ttacccagga gatgattgat acatccgagc gtattaaccg cacgatcggc
    24721 attgatattt cagactctgt aaccagtagc agaagtgatg ttgctgcaag tttgctggca
    24781 gttaaaaaag cctacgatct ggcgaaaagc aagtatacgg cgcaggatgc aagcacaacg
    24841 caaaagggat tagttcagct cagtagcgaa actaacagcg acagcgaaac aatggcggct
    24901 acccctaaag ccgttaagtc tgtaaaagat cttgctgata ccaaagcgcc aatagaaagc
    24961 ccgagtctga caggaacgcc aaccgcgccg acggcagcgc aaggtacaaa tagcacgcag
    25021 atcgcaaata cagcctttgt taaggcagct ataacggcac ttatcaacgg tgcacctggc
    25081 acactggata cgcttaaaga aatagctgct gcgatcaata acgacccgaa tttcagcaca
    25141 actatcaaca atgctctggc tcttaaagct cctttagcaa gtcctgcatt aacgggaata
    25201 cctactgcgc ctaccgctga acagggtacg aataacacgc agattgctac gaccgcttat
    25261 gtaagagctg ccatatccgc attggttggt tcatcaccag aagctcttga taccctgaat
    25321 gagcttgccg cagcacttgg caatgacccg aactttgcga caacaatgac aaatgcgctg
    25381 gcaggcaaac agcctctgga tgcaacttta accgctctcg ctgcccttgc gactggtgca
    25441 aacaaactgc cttatttcac tggtaaggat acggtagctc agactgattt aacgtcagtc
    25501 ggtcgcgata ttctggctaa aacaagcaca ctggccgtta tccaatacct tggtttaaga
    25561 gaacttggca caagcggtga aaagatcccc ctgttgagca cggctaacac atggagtgca
    25621 cgccagactt tcaatggtgg gatcaccggg gcgctggcgg ggaacgccga caccgcgacg
    25681 aaattaaaaa cagccagaaa cattaatggc gtcatgttcg atggttcggc tgacattaat
    25741 atcaatactc tggtatctcg cggtcgcgta acggccctgg aggcgaatgc acagggaaca
    25801 tccgggattc agctgtatga ggcatacaac aatggctacc cttcccccta tggcaatgtg
    25861 cttcacctta aaggtgccac cgctgctggc gaaggtgagt tattcattgg ctggagtggc
    25921 acgagcggtg cccatgcgcc cgtacatatc cgttcgcggc gggatactga ttctgccaac
    25981 tggtctgaat gggcgcaggt ctatacgtca aaagattcaa ttcccggcgt caatgccaaa
    26041 gggaatcagg acacctctgg taatgcggct acagcgacca agttgcagac agcatgtact
    26101 atcaatggcg tctcgtttga tggttctact gatatcactt taaccgctgc gcatgttgct
    26161 gcttttgcca gaagagcaac ggatacgtat gccgatgcgg atggtggcgt tccctggaat
    26221 gccgaatcag gcgcttacaa tgtcacccgc tctggcgaca gctatattct ggttaacttc
    26281 tataccggag tcggaagttg ccagaccttg cagatgaagg cgcattacag aaatggtggt
    26341 ctgttctacc gttcttcaag agacggttat ggttttgagg aagactgggc agaagtttat
    26401 acctcgaaaa atcttccaca agaaagctac ccagtcggcg caccaatccc gtggccatca
    26461 gataccgttc cgtctggtta tgccctgatg caggggcaga cttttgacaa atctgcctac
    26521 ccgaaacttg cagccgctta tccgtcaggc gtgatccctg atatgcgtgg ctggacgatt
    26581 aagggcaagc ccgccagcgg tcgagccgta ttgtctcagg aacaggacgg cattaaatcg
    26641 cacacccaca gcgccagtgc atccagtacg gatttgggga cgaaaaccac atcgtcgttt
    26701 gattacggta ctaaaacgac cagttcattt gattacggca caaaaaccac gaatagcgct
    26761 ggaaatcatt cacacaatat acctgttggt cacactggcg cggggaatgg tgtatcagcc
    26821 ggttataacg ctgcgttagg tactggtacc acgtcgagcg caggcgggca tgctcacaat
    26881 gtatatatcg gtgcccataa ccacactatc ggcattggtg ctcatgccca ttctgtcatt
    26941 attggtcccc acggacacac catcaccgtt aacgctacgg gtaacgaaga aaacaccgta
    27001 aaaaacatcg catttaacta tattgtgagg cttgcataat gacattcaga atgagtgaac
    27061 aatcacggac cataaaaatt tataatctac tggccggaac caatgagttt attggtaaag
    27121 gtgatgcata cattccacct catacaggtc tgcctgcaaa cagtaccgat attgccccgc
    27181 cagatattcc ggctggcttc gtggccgttt tcaacagtga tgaggcatcg tggaatctcg
    27241 ttgaagacca tcggggaaaa accgtctatg acgtggcttc cggcgacgcg ttatttattt
    27301 ctgaacttgg cccattaccg gaaaatgtca cctggttatc cccggaaggg gagtttcaga
    27361 agtggaacgg cacagcctgg gtgaaagatg cagaagcaga aaaactgttc cggatccgga
    27421 aggcggaaga aacaaaaaac agcctgatgc agatggccag tgagcatatt gcgccacttc
    27481 aggatgccgt agatttggat attgcgacgg aggaagaggc atcgttactg actgcatgga
    27541 agacatatcg ggtattgttg aatcgtgttg atacagcagt agcagcggat gttgagtggc
    27601 cagtcgcccc gcaataaaaa gataaagcca tcgatagaaa tattgatggc tttatgtact
    27661 ctatttatac aatacaacac cgctcttttt agtaatatat gtgcagttcg atggtatatc
    27721 tttatttata aaagacattg cacctatttt tacattatcc ccaattttac gtgataatcc
    27781 aatgatgcaa caattagctc cgatatcaac gttgctacca atttttactc ttgaaccagg
    27841 tatgtcacca tctatctgtc caatggtcgt attctgtcgt aacaccagat tttcaccagc
    27901 atcaacagca aaatgaacaa caattccagc atgatgggga attgttaacc cttttccaat
    27961 atttgcgccc aatccaattt cacaaccaaa tttgttaatt attttactgt ttaacttttt
    28021 ggctgctttc ttatgtaatt tattaccatt aatatgcatt tcattagcca atctccacca
    28081 gaaaaggaaa ttccgattgc gctgcctctt ctctcttaat agtcgccaaa catccatttt
    28141 ttcacgcctg atgacttcag ccatccaata gctcaataga gcttttgaat tcccaaagat
    28201 gacaaagtga atagctatta agtaacataa catattgca
//
LOCUS       DAZKHC020000059        28233 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZKHC020000059 DAZKHC020000000
VERSION     DAZKHC020000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.059"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            134..1177
                     /gene="nadA"
                     /locus_tag="ACHOSM_RS14475"
                     /old_locus_tag="pgaptmp_002895"
     CDS             134..1177
                     /gene="nadA"
                     /locus_tag="ACHOSM_RS14475"
                     /old_locus_tag="pgaptmp_002895"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HGU4559378.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELQEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            1215..1934
                     /gene="pnuC"
                     /locus_tag="ACHOSM_RS14480"
                     /old_locus_tag="pgaptmp_002896"
     CDS             1215..1934
                     /gene="pnuC"
                     /locus_tag="ACHOSM_RS14480"
                     /old_locus_tag="pgaptmp_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGU4559379.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(1931..2872)
                     /gene="zitB"
                     /locus_tag="ACHOSM_RS14485"
                     /old_locus_tag="pgaptmp_002897"
     CDS             complement(1931..2872)
                     /gene="zitB"
                     /locus_tag="ACHOSM_RS14485"
                     /old_locus_tag="pgaptmp_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HGU4559380.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(2986..3366)
                     /locus_tag="ACHOSM_RS14490"
                     /old_locus_tag="pgaptmp_002898"
     CDS             complement(2986..3366)
                     /locus_tag="ACHOSM_RS14490"
                     /old_locus_tag="pgaptmp_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HGU4559381.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3682..4734
                     /gene="aroG"
                     /locus_tag="ACHOSM_RS14495"
                     /old_locus_tag="pgaptmp_002899"
     CDS             3682..4734
                     /gene="aroG"
                     /locus_tag="ACHOSM_RS14495"
                     /old_locus_tag="pgaptmp_002899"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HGU4559382.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(4892..5644)
                     /gene="gpmA"
                     /locus_tag="ACHOSM_RS14500"
                     /old_locus_tag="pgaptmp_002900"
     CDS             complement(4892..5644)
                     /gene="gpmA"
                     /locus_tag="ACHOSM_RS14500"
                     /old_locus_tag="pgaptmp_002900"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HGU4559383.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(5846..6886)
                     /gene="galM"
                     /locus_tag="ACHOSM_RS14505"
                     /old_locus_tag="pgaptmp_002901"
     CDS             complement(5846..6886)
                     /gene="galM"
                     /locus_tag="ACHOSM_RS14505"
                     /old_locus_tag="pgaptmp_002901"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HGU4559384.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(6880..8028)
                     /gene="galK"
                     /locus_tag="ACHOSM_RS14510"
                     /old_locus_tag="pgaptmp_002902"
     CDS             complement(6880..8028)
                     /gene="galK"
                     /locus_tag="ACHOSM_RS14510"
                     /old_locus_tag="pgaptmp_002902"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGU4559385.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(8032..9078)
                     /gene="galT"
                     /locus_tag="ACHOSM_RS14515"
                     /old_locus_tag="pgaptmp_002903"
     CDS             complement(8032..9078)
                     /gene="galT"
                     /locus_tag="ACHOSM_RS14515"
                     /old_locus_tag="pgaptmp_002903"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HGU4559386.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(9088..10104)
                     /gene="galE"
                     /locus_tag="ACHOSM_RS14520"
                     /old_locus_tag="pgaptmp_002904"
     CDS             complement(9088..10104)
                     /gene="galE"
                     /locus_tag="ACHOSM_RS14520"
                     /old_locus_tag="pgaptmp_002904"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGU4559387.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSSALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(10365..11837)
                     /gene="modF"
                     /locus_tag="ACHOSM_RS14525"
                     /old_locus_tag="pgaptmp_002905"
     CDS             complement(10365..11837)
                     /gene="modF"
                     /locus_tag="ACHOSM_RS14525"
                     /old_locus_tag="pgaptmp_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HGU4559388.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(11905..12693)
                     /gene="modE"
                     /locus_tag="ACHOSM_RS14530"
                     /old_locus_tag="pgaptmp_002906"
     CDS             complement(11905..12693)
                     /gene="modE"
                     /locus_tag="ACHOSM_RS14530"
                     /old_locus_tag="pgaptmp_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HGU4559389.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            12822..12971
                     /gene="acrZ"
                     /locus_tag="ACHOSM_RS14535"
                     /old_locus_tag="pgaptmp_002907"
     CDS             12822..12971
                     /gene="acrZ"
                     /locus_tag="ACHOSM_RS14535"
                     /old_locus_tag="pgaptmp_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HGU4559390.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            13136..13909
                     /gene="modA"
                     /locus_tag="ACHOSM_RS14540"
                     /old_locus_tag="pgaptmp_002908"
     CDS             13136..13909
                     /gene="modA"
                     /locus_tag="ACHOSM_RS14540"
                     /old_locus_tag="pgaptmp_002908"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4559391.1"
                     /translation="MARKWLNLFAGAALSFTVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFIIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            13909..14598
                     /gene="modB"
                     /locus_tag="ACHOSM_RS14545"
                     /old_locus_tag="pgaptmp_002909"
     CDS             13909..14598
                     /gene="modB"
                     /locus_tag="ACHOSM_RS14545"
                     /old_locus_tag="pgaptmp_002909"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HGU4559392.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            14601..15659
                     /gene="modC"
                     /locus_tag="ACHOSM_RS14550"
                     /old_locus_tag="pgaptmp_002910"
     CDS             14601..15659
                     /gene="modC"
                     /locus_tag="ACHOSM_RS14550"
                     /old_locus_tag="pgaptmp_002910"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HGU4559393.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(15660..16478)
                     /gene="ybhA"
                     /locus_tag="ACHOSM_RS14555"
                     /old_locus_tag="pgaptmp_002911"
     CDS             complement(15660..16478)
                     /gene="ybhA"
                     /locus_tag="ACHOSM_RS14555"
                     /old_locus_tag="pgaptmp_002911"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HGU4559394.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            16633..17628
                     /gene="pgl"
                     /locus_tag="ACHOSM_RS14560"
                     /old_locus_tag="pgaptmp_002912"
     CDS             16633..17628
                     /gene="pgl"
                     /locus_tag="ACHOSM_RS14560"
                     /old_locus_tag="pgaptmp_002912"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HGU4559395.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(17669..18622)
                     /locus_tag="ACHOSM_RS14565"
                     /old_locus_tag="pgaptmp_002913"
     CDS             complement(17669..18622)
                     /locus_tag="ACHOSM_RS14565"
                     /old_locus_tag="pgaptmp_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4559396.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDEKDNLWTNIVERQRHALQEIS"
     gene            18806..19858
                     /locus_tag="ACHOSM_RS14570"
                     /old_locus_tag="pgaptmp_002914"
     CDS             18806..19858
                     /locus_tag="ACHOSM_RS14570"
                     /old_locus_tag="pgaptmp_002914"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HGU4559397.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            19934..21367
                     /locus_tag="ACHOSM_RS14575"
                     /old_locus_tag="pgaptmp_002915"
     CDS             19934..21367
                     /locus_tag="ACHOSM_RS14575"
                     /old_locus_tag="pgaptmp_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HGU4559398.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSTYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            21550..23811
                     /locus_tag="ACHOSM_RS14580"
                     /old_locus_tag="pgaptmp_002916"
     CDS             21550..23811
                     /locus_tag="ACHOSM_RS14580"
                     /old_locus_tag="pgaptmp_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HGU4559399.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(24045..25328)
                     /locus_tag="ACHOSM_RS14585"
                     /old_locus_tag="pgaptmp_002917"
     CDS             complement(24045..25328)
                     /locus_tag="ACHOSM_RS14585"
                     /old_locus_tag="pgaptmp_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HGU4559400.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSRRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DHDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(25463..26533)
                     /locus_tag="ACHOSM_RS14590"
                     /old_locus_tag="pgaptmp_002918"
     CDS             complement(25463..26533)
                     /locus_tag="ACHOSM_RS14590"
                     /old_locus_tag="pgaptmp_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4559401.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTALHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(26511..26729)
                     /locus_tag="ACHOSM_RS14595"
                     /old_locus_tag="pgaptmp_002919"
     CDS             complement(26511..26729)
                     /locus_tag="ACHOSM_RS14595"
                     /old_locus_tag="pgaptmp_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4559402.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(26820..27119)
                     /locus_tag="ACHOSM_RS14600"
                     /old_locus_tag="pgaptmp_002920"
     CDS             complement(26820..27119)
                     /locus_tag="ACHOSM_RS14600"
                     /old_locus_tag="pgaptmp_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559403.1"
                     /translation="MACSTFNPLTLQKYQPDPEDLCSLCGGNHGKAAMIECKDKIHIC
                     LNCVDVLVDIKNEREDKKRSEAVRALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
     gene            complement(27356..28054)
                     /locus_tag="ACHOSM_RS14605"
                     /old_locus_tag="pgaptmp_002921"
     CDS             complement(27356..28054)
                     /locus_tag="ACHOSM_RS14605"
                     /old_locus_tag="pgaptmp_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HGU4559404.1"
                     /translation="MKNILLASLLVVSPGAFAASFDCQKASTAIEHKICDNERLSKLD
                     EQLSSAYSSALKGNPENADTLKMVQRQWVNMRGKLTDNKALELAYLIQINGLKGLGSS
                     VSVTAANDIPTSAQKHSEEQEETSKAEAKSVKNGNELTLESFRAKYVEVDGEYYSTTS
                     IPRGSSFLFTCASRIADDQVNIWKKQAAKEGKIDLFFEVENHLHTAMLNANFQKLNSD
                     PAKRGICNLINAVP"
BASE COUNT         6744 a         6947 c         7226 g         7316 t
ORIGIN      
        1 ttaggcgtaa ttcgagtgac ttttccccac cattcgacta tcttgtttag catataaaac
       61 aaattacacc gataacagcg aatattacgc taatgtcggt tttaacgtta agcctgtaaa
      121 acgagatggt aagatgagcg taatgtttga tccagacacg gcgatttatc ctttcccccc
      181 gaagccgacg ccgttaagca ttgatgaaaa agcgtattac cgcgagaaga taaaacgtct
      241 gctaaaagaa cgtaatgcgg tgatggttgc ccactactat accgatcccg aaattcaaca
      301 actggcagaa gaaaccggtg gctgtatttc tgattctctg gaaatggcgc gcttcggtgc
      361 aaagcatccc gcttctactt tgttagtcgc tggggtgaga tttatgggag aaaccgccaa
      421 aattctcagt ccggaaaaaa caattctgat gccgacactt caggctgaat gttcactgga
      481 tctcggctgc cctgttgaag aatttaacgc attttgcgat gcccatcccg atcgtactgt
      541 cgtcgtctac gccaacactt ctgctgcggt aaaagcgcgc gcagattggg tggtaacttc
      601 aagcattgcc gtcgaactta ttgatcatct tgatagtttg ggtgaaaaaa tcatctgggc
      661 acccgacaaa catctggggc gttacgtgca aaaacagacg ggtggagaca ttctatgctg
      721 gcagggtgcc tgtattgtgc atgatgaatt taagactcag gcgttaaccc gcttgcaaga
      781 agaatacccg gatgctgcca tactggtgca tccagaatca ccacaagcta ttgtcgatat
      841 ggcggatgcg gtcggttcca ccagtcaact gatcgctgct gcgaaaacat tgccacatca
      901 gaggcttatt gtggcaaccg atcggggtat tttctacaaa atgcagcagg cggtgccaga
      961 taaagagtta caggaagcac caaccgcagg tgagggtgca acctgccgca gctgcgcgca
     1021 ttgtccgtgg atggccatga atggccttca ggccatcgca gaggcattag aacaggaagg
     1081 aagcaatcac gaggttcatg ttgatgaaag gctgcgagag agggcgctgg tgccgctcaa
     1141 tcgtatgctg gattttgcgg ctacactacg tggataacga ataataaggc gtaacgttac
     1201 gctttggggg aaagatggat ttttttagtg tgcagaatat cctggtacat ataccaatag
     1261 gggcaggcgg ttatgatctc tcatggatcg aagcggtagg cacgatcgcc gggttgctgt
     1321 gtattggcct tgccagtctg gagaagatca gcaactactt ctttggcctg atcaacgtca
     1381 ccttgtttgg cattattttc tttcagattc agctgtatgc cagcctgcta ttacaggtgt
     1441 ttttctttgc cgcgaatatt tacggttggt atgcgtggtc gcgacaaacc agtcagaacg
     1501 aggcggagtt gaaaattcgc tggttgccat tgccgaaggc actcagctgg ttggcggttt
     1561 gcgttgtttc gattggtctg atgacggtat ttatcaatcc ggtgtttgca tttttgaccc
     1621 gcgtggcagt catgatcatg caagcattag gattacaggt tgtgatgcct gaactgcaac
     1681 cggacgcttt cccgttctgg gattcatgca tgatggtgtt atctatcgtg gcaatgattc
     1741 tgatgacgcg taagtatgtg gaaaactggc tgttgtgggt gattattaac gtgattagcg
     1801 tcgttatttt tgcacttcag ggcgtttacg ccatgtctct ggagtacatc atcctgacct
     1861 ttattgcgct caacggcagc cggatgtgga tcaacagcgc acgtgaaaga ggctcacgcg
     1921 cgctgtccca ttaatggtga tgatgtgaat ggccagacac cccttcattc aggtggcagt
     1981 ctggcccatg acaaggttga tattccatct gaatggtggc gtgctcaatc tgatagtgat
     2041 ccatcaggta gtgttggatc tgatccaaca aggcatcgtg atcgtgtggc gggatcacct
     2101 gcacatgcag cgttatcacc ggcttctcgc ctaccatcca tacatgtaca tggtgcacat
     2161 tgcggacttc cgggatttcc cggcacatac gacgcttcag ttcagcgata tccagcgata
     2221 ccggtgcacc ttcaagtaat tcattcacgc tatctttcaa cagtcgccac gcgctacgca
     2281 gaaccagaag cgacaccact atcgagagaa tggggtcagc aggtgtccag ccggtccaga
     2341 taataatcaa cgcggcaata atagccccaa ccgaacccaa caggtccccg agcacatgca
     2401 atgccgctgc tcgtacgttg aggtttttct cttcgctgcc gtgatgaagt aaccagaaag
     2461 aaagtatatt tgccagcaac ccagccacgg ctattgccat catcatgccg ccctcgaccg
     2521 gacgcggcgt gcggaagcgt tctatcgcct cccagacaat caaaatggta attaccacga
     2581 gggcgatggc attcacaaac gccgcgagcg tggtcagtct tagccaaccg aaagtgtggc
     2641 gaatggtggg aggacgacgt gaaaattgca cggcgagtag ggcgaaaagc agggcggcag
     2701 tatcggtcaa catatgaccc gcatcggcca gcaatgccag agaaccagaa agaaaaccac
     2761 caacgacttc taccagcata aacccggcag tcacgccgaa agcatacaac aagcgacgag
     2821 cattattatc ttctggcagg tgtgaggacg tgtgtgagtg tgagtgcgcc atgacgagtc
     2881 atcccttaat tattctcact taactttatg acatcatact gcttttagaa gtgaaaaatt
     2941 aaaagggaga gactccgctc tcccattatt ggctattttg cagggttact gcgtggtacc
     3001 gtcggttttg gtatcgacat cattattgat gccatcaccg gtttgtacct ttttattgat
     3061 atccggacat cgaccatctt tgcacatggt gttcttgtgc tcttcatctt tggtcattcc
     3121 gtcattgttc attgaagaac catccgaatg cagcattgtg ccaccagaac cggtatttac
     3181 cccgttattg tcgacgttat ttggcgcgac attttcacgg gcgtcagggg ctacctggcc
     3241 cgcatcagct gcggcgtttg cctggccgtt attagtttgc gctccgctat cggcagccag
     3301 tgcggcaccg ctggcaaggc ttagagtggc agtcagaaat aatgtggcca gttttgtcat
     3361 tttcatagga tgctcctgtt atggtcgtta tgtcggataa cctcttccaa cagtgcattt
     3421 gcaggtgaat ataaggcatt ggtttaagat ttcagccagt ttatgaaacg cagcagagaa
     3481 tcttgaaata attaacaaac aaaggagtta cagttagaaa ttgtaggaga gatctcgttt
     3541 ttcgcgacaa tctggcgttt ttcttgctaa ttccaggatt aatccgttca tagtgtaaaa
     3601 ccccgtttac acattctgac ggaagatata gattggaagt attgcattca ctaagataag
     3661 tatggcaaca ctggaacaga catgaattat cagaacgacg atttacgcat caaagaaatc
     3721 aaagagttac ttcctcctgt cgcattgctg gaaaaattcc ccgcaactga aaatgccgcg
     3781 aatacggttg cccatgcccg aaaagcgatc cataagatcc tgaaaggtaa tgatgatcgc
     3841 ctgttggtgg tgattggccc atgctcaatt catgatcctg tcgcagcaaa agagtatgcc
     3901 actcgcttgc tggcgctgcg tgaagagctg aaagatgagc tggaaatcgt aatgcgcgtc
     3961 tattttgaaa agccgcgtac cacggtgggc tggaaagggc tgattaacga tccgcatatg
     4021 gataacagct tccagatcaa cgacggtctg cgtatagccc gtaaattgct gcttgatatt
     4081 aacgacagcg gtctgccagc ggcgggtgag tttctcgata tgatcacccc acaatatctc
     4141 gctgacctga tgagctgggg cgcaattggc gcacgtacca ccgaatcgca ggtgcaccgc
     4201 gaactggcat cagggctttc ttgtccggtc ggcttcaaaa atggcaccga cggtacgatt
     4261 aaagtggcta tcgatgctat taatgccgcc ggtgcgccgc actgcttcct gtccgtaacg
     4321 aaatgggggc attcggcgat tgtgaatacc agcggtaacg gtgattgcca tatcattctg
     4381 cgcggcggta aagagcctaa ctacagcgcg aagcacgttg ctgaagtgaa agaagggctg
     4441 aacaaagcag gcctgccagc acaggtgatg atcgatttca gccatgctaa ctcgtccaaa
     4501 caattcaaaa agcagatgga tgtttgtgct gacgtttgcc agcagattgc cggtggcgaa
     4561 aaggccatta ttggcgtgat ggtggaaagc catctggtgg aaggcaatca gagcctcgag
     4621 agcggggagc cgctggccta cggtaagagc atcaccgatg cctgcatcgg ctgggaagat
     4681 accgatgctc tgttacgtca actggcgaat gcagtaaaag cgcgtcgcgg gtaaggttta
     4741 attgtcggat gcgccgtcag agtggcgtat ccgatgaatc accacaggcc tgataagacg
     4801 cgcagcgtcg catcaggcaa tgtgctccat tgttagcaac aaaaaagccg actcacttgc
     4861 agtcggcttt ctcattttaa acgaatgacg tttacttcgc tttaccctgg tttgcaaccg
     4921 ccgctgcttt cgctgcgatc tcgtcagcat tacccagata atagcgtttc agcggtttga
     4981 aattctcgtc gaactcatac accagcggca cgccagtcgg gatattcagc tcaagaatct
     5041 cttcttcgct catgttatca agatatttca ccagcgcacg cagggagtta ccgtgtgcgg
     5101 cgatgattac gcgctcaccg ctcttcatac gcggtagaat agtttcattc cagtaaggga
     5161 tcacgcggtc aatggtcagc gccaggcttt ccgtcagcgg cagttctttc tcgctcagtt
     5221 tcgcgtaacg cggatcgtga cccggataac gctcatcatc tttagtcagt tccggcggag
     5281 tcactgcaaa accacgacgc cactgtttca cctgctcgtc gccatacttt tcagcagttt
     5341 ccgctttgtt cagaccctgc aacgcaccgt agtgacgttc gttcagtttc caggatttct
     5401 caacgggcag ccatgcctga tccagttcgt ccagcacatt ccacagggta tggatagcgc
     5461 gtttcagcac agaagtgtaa gcaaagtcaa agctgtaacc ttcctctttc agcagcttac
     5521 ctgctgcttt tgcttcgctt acgcctttct cagacagatc cacgtcgtac caaccggtga
     5581 aacggttttc tttgttccac tgactttcgc catgacgaac cagaaccagc ttagttacag
     5641 ccatatactt actcctcaaa tcatctttta atgataataa ttctcattat attgccgcga
     5701 cgaagcaaca gcaatgctta cgcataacca tagcgaaaat agtggcgcag tgtaaggttg
     5761 ttgtgaatat tgagttgcaa atatgtcggt gtttgctggt gatttgaaca atatgagata
     5821 aagccctcat gacgagggct taacattact cagcaataaa ctgatattcc gtcaggctgg
     5881 aatactcttc gccaggacgc aggaagcagt ccggttgcgg ccattcaggg tggttcgggc
     5941 tgtccggcag aaactcgctt tccaacgcca gcccttgcca gtcggcgtaa ggttcggttc
     6001 cccgcgacgg tgtgccgccg aggaagttgc cggagtagaa ttgcagagcc ggagcggtgg
     6061 tgtagacctt cagctgcaat ttttcatctg ctgaccagac atgcgccgcc actttcttgc
     6121 catcgccttt ggcctgtaac aagaatgcgt gatcgtaacc tttcactttg cgctgatcgt
     6181 cgtcggcaag aaactcactg gcgatgattt tggcgctgcg gaaatcaaaa gacgttccgg
     6241 cgacagattt caggccgtcg tgcggaatgc cgccttcatc aaccggcaga tattcgtccg
     6301 ccagaatctg caacttgtga ttgcgcacgt cagactgctc gccgtcaaga ttgaaataga
     6361 cgtgattagt catattcacc gggcaaggtt tatcaactgt ggcgcgataa gtaatggaga
     6421 tacggttatc gtcggtcaga cgatattgca ccgttgcgcc gagattaccc gggaagccct
     6481 gatcgccatc atctgaactc agggcaaaca gcacctgacg atcgttctgg ttcacaatct
     6541 gccagcgacg tttgtcgaac ccttccgggc cgccgtgcag ctggttaacg ccctgacttg
     6601 gcgaaagggt cacggtttca ccgtcaaagg tataacggct attggcgatg cggttggcat
     6661 aacgaccaat agaggccccc agaaacgcgg cctgatcctg atagcattcc gggctggcac
     6721 agccgagcag cgcctcgcgg acgctgccat cggaaagcgg aatacgggct gaaagtaaag
     6781 tcgcacccca gtccatcagc gtgactacca tccctgcgtt gttacgcaaa gttaacagtc
     6841 ggtacggctg accatcgggt gccagtgcgg gagtttcgtt cagcactgtc ctgctccttg
     6901 tgatggttta caaacgtaaa aagtctcttt aatacctgtt tttgcttcat attgttcagc
     6961 gacagcttgc tgtacggcag gcaccagctc ttccgggatc aacgcgacga tacagccgcc
     7021 aaatccgccg ccggtcatgc gtacgccacc tttgtcgcca atcacagctt tgacgatttc
     7081 taccagagtg tcaatttgcg gcacggtgat ttcgaaatca tcgcgcatag aggcatgaga
     7141 ctccgccatc aactcgccca tacgtttcag gtcgccctgc tccagcgcgc tggcagcttc
     7201 aacggtgcgg gcgttttcag tcaggatatg ccgcacgcgt ttcgccacga ttgggtccag
     7261 ctcatgtgca acagcgttga actcttcaat ggtgacatcg cgcagggctg gctgctggaa
     7321 gaaacgcgca ccggtttcgc actgttcacg acgggtgttg tattcgctgc caaccagggt
     7381 acgtttgaag ttactgttga tgatgacgac agccacacct ttcggcatgg aaactgcttt
     7441 ggtccccagt gaacggcagt caatcagcaa ggcatgatct ttcttgccga gcgcggaaat
     7501 tagctgatcc atgatcccgc agttacagcc aacaaactgg ttttctgctt cctgaccgtt
     7561 aagcgcgatt tgtgcgccgt ccagcggcag atgataaagc tgctgcaata cggttccgac
     7621 cgcgacttcc agtgaagcgg aagaacttaa cccggcaccc tgcggcacat tgccgctgat
     7681 caccatgtcc acaccgccga agctgttgtt acgcagttgc agatgtttca ccacgccacg
     7741 aacgtagttc gcccattgat agttttcatg cgcgacaatg ggcgcattga gggaaaactc
     7801 gtcgagctga ttttcataat cggctgccat cacgcgaact ttacggtcat cgcgtggtgc
     7861 acagctgatc acggtttgat aatcaatcgc gcagggcaga acgaaaccgt cgttgtagtc
     7921 cgtgtgttca ccaatcaaat tcacgcggcc aggcgcctga atggtgtgag tggcagggta
     7981 gccaaatgcg ttggcaaaca gagattgtgt tttttctttc agactcattt cttacactcc
     8041 ggattcgcga aaatggatat cgctgactgc gcgcaaacgc tctgctgcct gttctgcggt
     8101 caggtctcgc tgggtctctg ccagcatttc ataaccaacc ataaatttac gtacggtggc
     8161 ggagcgcagc agaggcggat aaaagtgcgc gtgcagctgc cagtgttgat tctcttcgcc
     8221 attaaatggt gcgccgtgcc agcccataga gtaggggaag gagcactgga agaggttgtc
     8281 ataacgactg gtcagctttt tcaacgccag cgccaaatcg ctgcgctggg cgtcggtcaa
     8341 atcggtgatc cgcaaaacgt gggctttggg cagcagtagc gtttcgaacg gccaggcagc
     8401 ccagtaaggc acaacggcta accagtgttc ggtttcgaca acggtacggc taccgtctgc
     8461 cagctcgcgc tgaacataat ccaccagcat tggtgatttc tgttcggcaa aatattcttt
     8521 ttgcaggcgg tcttcgcgct cagcttcgtt aggcaggaag ctatttgccc aaatctgtcc
     8581 gtgcggatgc gggttagagc agcccatcgc cgcacctttg ttttcaaaga cctgcaccca
     8641 cgggtacgtt tttcccagct ctgcggtttg ctcctgccag gttttgacga tttccgtcaa
     8701 tgccgcaacg ctcagttctg gcaacgtttt actgtgatcc ggtgaaaagc agatcacccg
     8761 gctggtaccg cgcgcgctct ggcaacgcat caaaggatcg ttgctttctg gcgcatctgg
     8821 cgtgtcagac atcaaggccg caaagtcatt agtgaaaacg taagtcccgg tgtaatcggg
     8881 gtttttatcg cctgtcaccc gcacattacc tgcgcagagg aagcaatctg gatcgtgcgc
     8941 aggtaacacc tgtttggctg gcgtttcctg cgccccctgc caggggcgct tagcgcggtg
     9001 cggtgaaacc agaatccatt gcccggtgag cgggttgtag cggcgatgtg gatgatcaac
     9061 gggattaaat tgcgtcatgg tcgttcctta atcgggatat ccctgtggat ggcgtgactg
     9121 ccagtgccag gtgtcctgcg ccatttcatc gagtgtgcgc gttacgcgcc agttcagttc
     9181 acggtcggct ttgctggcgt ccgcccagta ggccggaagg tcgccttcgc gacgcggtgc
     9241 aaaatgatag ttaaccggtt tgccgcaggc tttgctgaag gcattaacca cgtccagcac
     9301 gctgctgcct acgccagcac cgaggttgta gatgtgtacg cctggcttgt tcgccagttt
     9361 ttccatcgcc acgacgtgac cgtccgccag atccattacg tggatgtaat cgcgtacgcc
     9421 agtaccatct tcggtcggat aatcgttacc aaaaatcgcc agcgagtcgc gacggcctac
     9481 agcaacctgg gcgatgtatg gcatcaggtt attcggaatg ccttgcggat cttcgcccat
     9541 atcgcccgac ggatgcgcgc caaccgggtt gaagtagcgc agcagggcac tgctccagtc
     9601 cggctgggct ttttgcaggt cggtgaggat ctgttctacc atcaatttgc ttttgccata
     9661 agggctttgc ggtgtgccgg tcgggaagct ttcaacgtat ggaattttgg gctgatcgcc
     9721 ataaacggtg gcggaggagc taaaaataaa gtttttgacg ttagcggcgc gcatggcgct
     9781 aatcaggcgc agagtgccgt tgacattgtt gtcgtaatat tccagcggtt tttgtaccga
     9841 ttcgccgacg gctttcagcc cggcgaagtg gatcacggtg tcgatagcgt gatcgtgcag
     9901 tatctcggtc atcaacgctt cgttacgaat atcgccttca acaaacgttg gatgtttgcc
     9961 gcctaaacgc tcgataacag gcagtacgct gcgcttactg ttacagaggt tatcaagaat
    10021 gatgacatca tgaccgtttt gcagtaattg cacgcaggta tgacttccaa tgtaaccgct
    10081 accaccggta accagaactc tcataattcg ctccattagg cttatggtat gaaataacca
    10141 tagcataaca aagatgcgaa aagtgtgaca tggaataaat tagtggaatc gtttacacaa
    10201 gaatttagcc gttttttatg cgcgattaag tgattataaa acagagggtt tatgaatgat
    10261 tgcgcttttt atctgaaaaa agaagcggtt tcatgcctgc atgcgtcgaa ccgttggccg
    10321 gagagggcgc ttaggccgcc tccggcaagg tcagcactac cgactcaata tatttttgtc
    10381 agcacatagc gatagagtcc accgtccggc acgaactcaa ggcgatgggt aatacaggca
    10441 ggcgcatctt cagcgtggtg cgaaacaaac aacaattgcg tttcaccttc gctaatcagc
    10501 acatcaacaa aacggcggat aagctggcga ttgagcggat caagcccctg tagtggttca
    10561 tcgagaataa gcaacgtcgg atgtttcacc agtgcgcgga cgatcagcgc cagacgctgc
    10621 tgtccccagg aaagactatg gaacggagcg tcagccgtac gtttatcaat gccgagaata
    10681 tccagccact gctgcaccag tttttgctgg cgatccgaaa cggcctgata aatgccaatc
    10741 gaatcaaaat agccagaaag aatcacatta cgcacggtag tgctgacccg gtaatccaga
    10801 tgcaaactac tgctgacgta accgatatgc tttttgatat cccagatggt ttcgccgctg
    10861 ccgcgacgtc gtccgaaaag cgtcaaatcg ttgctgtaac cttgcggatg atcgccagta
    10921 accaggctta ataacgtcga ttttcctgca ccatttggcc cgacaatttg ccagtgttca
    10981 cctggattca cctgccagct aaggttatta agaatggggc gatcgttata agaaaccacg
    11041 ccattgttca gcacaatgcg cggttcgttg gcgggtaagg cgtgacgtgc tgaaggttca
    11101 tccggctccg gcagttgcac accttcaagc tgttcactat gcgccagttg cgcgacgagt
    11161 gcttgttgga gcagttcctc tttagcgcca gtttccgcta acgtgcaatc cgccagcacg
    11221 ccagcaaact ggacaaactc cgggatctca tcgaagcgat tgagcaccag taccagagta
    11281 ataccggact gatgtaacga ggcgagtcgc tcagccagct gctgacgtga ggcaacatcc
    11341 aggccatcga acggctcatc aagaatcaac aagtcaggct ccgacatcag tgcctgacac
    11401 agcagggttt ttcgcgtctc gccagtggaa aggtatttaa agcgtcggtc gaggagggca
    11461 gtaataccga actgctgcgc cagttgcatg caacgcggtg catcctttac ttcatcctga
    11521 atgatctcag ccgtagtgcg tccggtgtca tcttcgccag ggccgagcat atcggtgtta
    11581 ttccgctgcc attcgtcgct gacgagtttt tgcaattgct cgaaggagag acgagtgatg
    11641 tgggaaaact ggctttgccg ttcacctttc aaaagcggaa gttcccccgc cagcgcgcgg
    11701 gccagggccg atttcccgct tccattcgaa ccgacaaacg cccaactatc acccgcgttt
    11761 aacgttagct gaggcaattg cagcgttttt gtgtcgctaa gacgaaacgt gccttgcaaa
    11821 atttgcaacg atgacatttt atatcccatt ttgtgcagcg attactgaca gggatacgtg
    11881 tttcataaca aattgtcaac acgcttagca cagcgtggcg ataatcacgc tgtcggcatt
    11941 aaagtaggcc gtgacattct gcccttgctg aagagaagtc gcttcattta ccggcactgt
    12001 ggcgcacagt gtttgcccgt cgggtagcgc cattaatact tcgcactgct ctgcgccgcg
    12061 ctcaatatga ctaataatac ccggtaattg gttgtcagcg ttttgcgcga ccgcctcgtc
    12121 ctgagtaata cctacccacg gcgcttttag caatatcaac acttctttgc cttcatccag
    12181 ccccagacgc gcgccgcttt gtgcggtaat tgcgactttc aggcgtgttt ttccgtcagc
    12241 cagtaagaca tcaacatgct gttgaacgtc atcatgatcg cgggcggtga tggtgccgaa
    12301 ccactggtta cgggcgctgg tttgcagtga aaaacgtgag atcgcggcca gcaggctgtt
    12361 cagcggcagg gcgtcatcgt cacttaacac atcaaaggct ttttgctgga tttgcgccag
    12421 taagtcatag agctgaatca gtcgctgacc atagcgggtc agtactgcgc cgccgccacc
    12481 tttaccgcct gttgcgcgct cgaccagaat atgctcactt aactgattca tctcgttaat
    12541 ggcatcccag gcgcttttat agctgatacc ggcatctttc gctccctggc taatggaacc
    12601 ggaaagcgca atgtgtttta gtagcgaaat ccggcgcggg tcggcgaata atttttgttg
    12661 gagcttaagg gtgagaagga tttcggcctg cataacaatg tcctggcaaa agtcttattg
    12721 tgacggaaaa cgaacgccac gcaaagctga ccgcacaaaa ggggagtgct tttctgtgct
    12781 tagcggttag aatagtctca tgactatatc tggagttgac catgttagag ttattaaaaa
    12841 gtctggtatt cgccgtaatc atggtacctg tcgtgatggc catcatcctg ggtctgattt
    12901 acggtcttgg tgaagtattc aacatctttt ctggtgttgg taaaaaagac cagcccggac
    12961 aaaatcattg attccctgaa tgcccgctta gtcgggcatt tctttttctc aacttcctgc
    13021 ttttcctgcc gatatttttt ctatctacct ctcaaaggtt aacaataact gctgggaaaa
    13081 ttctgaactt accgctatat tgacgcctac ataacgttaa ataaaggggt tacaaatggc
    13141 tcgtaaatgg ttaaacctgt ttgccggggc ggcgctctct ttcactgttg ctggcaatgc
    13201 gttggcggat gaagggaaaa tcacggtttt tgccgcagca tcactgacca acgcgatgca
    13261 ggatatcgct actcagtata aaaaagaaaa aggcgtggat gtggtttctt ctttcgcttc
    13321 gtcatctact ctcgcccgtc agattgaagc gggtgcgcct gcggatctgt ttatttctgc
    13381 cgatcagaaa tggatggatt atgcggttga taaaaaagcg atcgatacag ctacgcgtca
    13441 gacactgctc ggcaatagcc tggtcgttgt agcaccgaaa gccagcgagc agaaagattt
    13501 catcatcgac agcaaaacta actggacttc gctgctgaat ggcggtcgcc tcgcggttgg
    13561 cgatccggaa catgtccctg ctggcattta tgcaaaagaa gcactgcaaa aactgggcgc
    13621 atgggatacg ctctccccga aactggctcc ggcggaagat gttcgtgggg cgctggcgct
    13681 ggtcgaacgt aatgaagcgc cgctgggtat tgtctacggt tctgacgcag ttgccagcaa
    13741 aggggtaaaa gtggtcgcca ccttcccgga agattcgcat aaaaaagtgg aatatccggt
    13801 tgccgtcgtg gaagggcata acaacgcgac agtgaaagca ttttatgatt atttgaaagg
    13861 gccgcaggct gcggaaatct ttaaacgtta cggatttaca actaagtaat gatactgacc
    13921 gatccagaat ggcaggcagt tttattaagc ctgaaagttt cttccctggc tgtgctgttt
    13981 agcctgccgt ttgggatctt ttttgcctgg ttactggtgc gttgcacgtt tccgggcaaa
    14041 gctctgctcg acagcgtact gcatctaccg ctggtgttac cgcccgtggt cgtcggttac
    14101 ttattattag tttcgatggg acggcgcggg tttatcggtg aacgtctgta tgactggttt
    14161 ggcattacct tcgcctttag ctggcgcggc gcggttctcg ctgccgccgt catgtcgttt
    14221 ccgctgatgg tgcgggcaat tcgtctggcg ctggaagggg ttgatgtcaa actcgaacag
    14281 gccgcaagaa cactaggtgc cgggcgctgg cgcgttttct ttactatcac gttaccgctg
    14341 accttaccgg gaattattgt tggtacggta ctggcttttg ctcgttctct cggtgagttt
    14401 ggtgcaacca tcacctttgt gtcgaacatt cctggcgaaa cgcgaaccat tccctctgcc
    14461 atgtataccc tgatccagac ccccggcggg gaaagtggcg cggcgagact gtgcattatt
    14521 tctattgcgc tggcgatgat ctccctgttg atttcagaat ggctggccag aatcagccgt
    14581 gaacgggcgg ggcgctaatc atgctggagc tgaatttttc ccagacgttg ggcaaccatt
    14641 gcctgaccat taatgaaacg ctgcccgcca atggcatcac tgctatcttt ggcgtctccg
    14701 gtgccggaaa aacttcgctg attaacgcca tcagtggact gacgcgcccg caaaaagggc
    14761 ggattgtcct caatgggcgg gtactaaatg atgccgaaaa aggtatctgc ctgacgccgg
    14821 aaaagcgtcg cgttggctat gtttttcagg atgcgcggct gttcccgcat tacaaagtgc
    14881 gtggcaatct gcgctacggc atgtcgaaaa gtatggtcga tcagttcgat aagctggtgg
    14941 cgcttttagg cattgaaccg ttgcttgacc gtttaccagg cagcctgtcc ggaggcgaaa
    15001 aacagcgcgt ggcgattggt cgtgctttgc tgacagcacc ggaattgctg ttgctggatg
    15061 aaccgctggc gtcactggat attccgcgta aacgcgaact gttgccttat ctgcaacgtc
    15121 tgacgcggga aatcaacatc ccgatgttgt atgtcagcca ttcgctggat gagatcctcc
    15181 atctggcaga cagagtgatg gtactggaaa acggtcaggt gaaagccttt ggcgcactgg
    15241 aagaagtctg gggtagtagc gtgatgaatc cgtggctgcc gaaagagcaa caaagtagca
    15301 ttctgaaagt gacggtactt gagcatcatc cgcattacgc gatgactgcg ctggcgctgg
    15361 gcgatcagca tttgtgggtc aataagctgg acgaaccgct gcaagctgcg ctacgtatcc
    15421 gcattcaggc ttccgatgtt tctctggttt tacaaccgcc gcagcaaacc agcattcgta
    15481 acgtattgcg ggcaaaagtt gttaatagtt atgacgacaa cggccaggtg gaagtggagc
    15541 tggaagtcgg cggtaaaacg ctgtgggcgc gtatcagccc gtgggctagg gatgaactgg
    15601 cgatcaaacc tggcctgtgg ctgtacgcgc aaattaaaag tgtgtcgata accgcctgat
    15661 taaatcaggt ggctataaat gaactgggca atgctgtcgg tggtgttatc accaatcaca
    15721 atgttggcgc gcgcttttac cgcgtcatcg gcgttgccca tcgccacgcc tgtaccagcg
    15781 gcttccagca tactgatatc attaaagtta tcgccgaatg ccacgacgtt ttccatcgac
    15841 caaccttgcg cctcaaccca tttcgtcaaa cgtttacctt tgctgttgcc gccgcgtgca
    15901 atatcaacct gatcgtgcca ggaccattca cactccagtc ccagttcatg ttcgacatgc
    15961 ttaccaaaat gctgcaattg cggcaggtca tcgtgcgtca gggcgaactt ccatacggcg
    16021 ttaacttgtt gcgccgtttc agccagagaa gcgacttgtg tgaaagtcgg acgctgttcc
    16081 ggcggcaggg tttgcgccca gttagacgtg cgaatgacat gcccggtcgg gtgctcatag
    16141 accattgcat catccacata catcagaccg tgaatgtggt gttcattcag catctcaatg
    16201 agttgcaggg ctttattaac gggcattggg tccgcttcca gcacggtttt tgcatgataa
    16261 tcatacaaat aggtgccatt acagcaaata gcaggtgtat ccagcgccag cgcctgataa
    16321 aaaggatgaa tagcgacgtg atggcgacct gtgacgatga ttaattgata gcctgcttcg
    16381 cgagcgcggg ccagggcttc tatcgatgaa ggaagcaggg tctttttcgg ggtcaataag
    16441 gtgccgtcta agtcgagagc aatcacgcgt gtggtcatgg cgtattccag attaaggtta
    16501 agaattttcg tctgcgcgaa tggtacaccg ataccactat cggacaaaat tctgcgtttt
    16561 aattcagcat tcaccgccaa aagcgactaa ttttagctgt tacagtcagt tgctaaatgc
    16621 aaaggagcat tcatgaagca aacagtttat atcgccagcc ctgagagcca gcaaattcac
    16681 gtctggaacc tgaatcatga aggcgcactg acgctgacac aggttgtcga tgtgccgggg
    16741 caggtgcagc cgatggtggt cagcccggac aaacgttatc tctatgttgg tgttcgccct
    16801 gagtttcgcg tcctggcgta tcgtatcgcc ccggacgatg gcgcactgac ctttgccgca
    16861 gagtctgcgc tgccgggtag tccgacgcat atttccaccg atcaccaggg gcagtttgtc
    16921 tttgtaggtt cttacaatgc gggtaacgtg agcgtaacgc gtctggaaga tggcctgcca
    16981 gtgggcgtcg tcgatgtggt cgaggggctg gacggttgcc attccgccaa tatctcaccg
    17041 gacaaccgta cgctgtgggt tccggcatta aagcaggatc gcatttgcct gtttacggtc
    17101 agcgatgatg gtcatctggt ggcgcaggac cctgcggaag tgactaccgt tgaaggggcc
    17161 ggcccgcgtc atatggtatt ccatccaaac gaacaatatg cgtattgcgt caatgagtta
    17221 aacagctcag tggatgtctg ggaactgaaa gatccgcacg gcaatatcga atgtgtccag
    17281 acgctggata tgatgccgga aaacttctcc gacacccgtt gggcggctga tattcatatc
    17341 accccggatg gtcgccattt atacgcctgc gaccgtaccg ccagtctgat taccgttttc
    17401 agcgtttcgg aagatggcag cgtgttgagt aaagaaggct tccagccaac ggaaacccag
    17461 ccgcgcggct tcaatgttga tcacagcggc aagtatctga ttgccgctgg gcaaaaatct
    17521 caccacatct cggtatacga aattgttggc gagcaggggc tactgcatga aaaaggccgc
    17581 tatgcggtcg ggcagggacc aatgtgggtg gtggttaacg cacactaacc gctgatttaa
    17641 ccggcgcagt ctctcctgcg ccggtgtatt aacttatctc ctgtaacgca tgtctctggc
    17701 gttcgacgat attggtccac aaattgtctt tctcgtcagt ccacaaatta atcagcaagg
    17761 caaaaaagcg ttcagctgcc ggagaaagca cggcatcttt gcggcgaata atccccaatg
    17821 tccgacgtat aacgggttca accagtggga taccaataat cgaagaatag ggcgcatggg
    17881 gcattgccag accaggaagc gccgaaatcc ccagtcctgc ctccactagt cctaatgacg
    17941 tcgaaagatg acgcacttcg taaaaccagt ccagcttcca gggcttgtcg gctagctgtt
    18001 gttctatcaa caatcggttg ccgctggagg aacgtacgcc aatcattttg taacccacta
    18061 actcctgcca ttctacaagc tgctttttgg ccaatggatg gtcacgccga caggccagca
    18121 cgaatggctc gttgaccagt ggggtaaaat caatggatga atttgtgacg ttgttcatgt
    18181 ttatgccaaa gtctgattcg ttacacagca ccgattccat gcaattattc gtgccttgtt
    18241 ccagaatccg cactttaata ttgggataca gctcattaaa tttaccgatt gccagcggta
    18301 aaaaataaaa tactgcggtc ggaatacacg ctaatgtcac cataccacga tgataagcat
    18361 tcatatcacg aatattaaaa agcgtttcat cgaatttctt tattaattcc cgcgcctccg
    18421 ggagcaacct ttttcctgct tttgttaacg taaccttgcg agttgtacgc tcaaatagtt
    18481 gaatatgtaa atcttcctcc atctttttta ttcttcgcgt taatgcaggt tgcgtaatat
    18541 tgagtaattt agcggcattg ttaaatgaac tggactctgc cagtatgaca aatgccttca
    18601 tacttgataa ttcatgcttc attattactc cggaaaatgg aagcgacgat tttgggtggc
    18661 tggtcgttaa aaattttaac tgcatttagc caacttaaat taatgaaaaa atgttattaa
    18721 tcgttgagct aaagtcatta gagatgcttt gtccttaatg taaccatatc gcaataagtt
    18781 atgtttttaa attgagggca ttattatgaa aaaaataccc tgcgtgatga tgcgaggtgg
    18841 aacctcgagg ggcgcgttcc tgttagcgga acatttaccc gaagatcaaa cgcagcgcga
    18901 taaaatattg atggcaatta tgggttccgg taacgatctg gaaattgacg gtattggcgg
    18961 cggtaatcca ctgaccagta aagtcgctat tattagccgt tccagcgatc cgcgtgctga
    19021 tgtcgattat ctgtttgcgc aggtaatcgt tcatgagcaa cgtgtcgata ccacgcctaa
    19081 ctgcggcaat atgctgtctg gtgttggggc attcgccatt gaaaatggtt tgattgcagc
    19141 gacttcgcca gttactcgcg tacgtatccg caacgtcaat acgggtacgt tcatcgaagc
    19201 tgatgtgcaa acgccaaatg gtgttgtcga gtacgagggt agtgccagaa ttgacggcgt
    19261 accgggtact gccgcaccgg ttgcgctcac tttcctgaat gccgctggaa ccaaaaccgg
    19321 aaaagttttc ccgactgata atcagattga ttattttgac gatgtcccgg tgacctgtat
    19381 cgatatggcg atgccagtcg tcattattcc ggctgaatat ctgggaaaaa caggttatga
    19441 attaccggcg gaactggatg ccgacaaagc attattagcc cgcattgaat ctatccgtct
    19501 acaagcgggt aaagcaatgg gcttaggtga tgtcagtaat atggttatcc ctaaacctgt
    19561 gcttatttct ccagcgcaga aaggcggggc aattaatgtg cgttatttta tgccgcattc
    19621 ttgccatcgc gcgctggcga taaccggtgc tattgctatt tccagtagtt gtgcattgga
    19681 aggcaccgtc acccgacaaa tcgtcccttc tgtaggatat ggcaatatca atattgaaca
    19741 ccccagtggt gcgctcgacg ttcatttaag taatgaaggt caggatgcca cgacgttacg
    19801 cgcatctgtt attcggacaa ccagaaaaat attttccggt gaagtttatc ttccctgaaa
    19861 aaattcgttg tcaggataag gacaatcaat aaaggacttc tgtatgagtc atacagaaag
    19921 aacaggattt taaatgaata agaaatcgtt atggaagcta attctgatat tagcgatccc
    19981 atgtattatt ggttttatgc cagctccggc aggattaagc gaactggcgt gggtgctttt
    20041 tggtatttac ctggcggcca ttgtgggtct ggttatcaag cctttcccgg aacctgtcgt
    20101 actgttaatt gccgttgctg cctcaatggt ggtggtcggt aacttatccg acggtgcgtt
    20161 taagaccacc gccgtattaa gcggttactc ttcaggtact acctggctgg tgttctcggc
    20221 gtttacctta agcgccgcat ttgtgaccac cggtttaggt aaacgtattg cctatctgct
    20281 gattggtaaa atcggtaaca ccacgctggg tctgggttac gttacggtat tcctcgatct
    20341 ggtactggct ccggcaacac cgtctaacac cgcgcgtgcg ggcggcattg tgttaccgat
    20401 catcaacagc gtggcggtgg ctttggggtc cgaaccggaa aaaagtccgc gtcgtgtcgg
    20461 acattacctg atgatgtcca tttacatggt caccaaaacc accagctata tgttctttac
    20521 cgcaatggcg gggaacattc tggcgctgaa aatgatcaac gacattctgc acctgcaaat
    20581 tagctggggt ggatgggcgc tggccgccgg attgccgggc atcattatgc tgctggtcac
    20641 cccgctggta atttacacca tgtatccacc agaaattaaa aaggtggata acaaaaccat
    20701 cgctaaagcg ggccttgccg aactgggacc gatgaaaatc cgcgaaaaaa tgctgctcgg
    20761 tgtctttgtg ctggcgctac tgggctggat tttcagtaag tctctggggg ttgatgaatc
    20821 cactgtggca atcgttgtta tggcgaccat gctgctgctg ggtatcgtta cctgggaaga
    20881 cgtggttaaa aataaaggcg gctggaatac cttaatctgg tacggcggta ttatcggctt
    20941 aagctcctta ttatcgaaag ttaaattttt cgaatggtta gctgaagtct ttaaaaataa
    21001 cctggcattt gatggtcacg gtaacgttgc tttcttcgtt attattttcc tcagcattat
    21061 cgtgcgttat ttcttcgctt ccggtagtac ctatatcgtt gctatgttac cggtatttgc
    21121 catgctggcg aacgtctccg gcgcaccgtt aatgttaacc gcgctggcac tgttgttctc
    21181 caactcctat ggcggcatgg ttactcacta tggcggcgcg gcaggtccgg tcatctttgg
    21241 cgtgggttat aacgatatta aatcctggtg gttggtcggt gcggtactga cgatattaac
    21301 cttcctggtg catatcaccc tcggcgtgtg gtggtggaat atgctgatcg gctggaacat
    21361 gctgtaaata tactcgtcat acttcaagtt gcatgtgctg cgtttgcgtt cgctcacccc
    21421 agtcacttac ttatgtaagc tcctggggat tcactcactt gtcgccttcc tgcaactcga
    21481 attatttaga gtatatccat ttattatctt tctgcgcact tcacggtgcg cagatatctg
    21541 gagcatttga tgatcaagtt atctgaaaaa ggcgtgtttc ttgccagtaa taacgaaata
    21601 attgccgaag aacatttcac cggcgaaatt aaaaaagaag aagccaaaaa aggcactatt
    21661 gcctggtcta ttctctcttc tcataatacg tccggaaata tggataaact taaaattaag
    21721 tttgattcat tagcctctca cgatattacc tttgttggta ttgtacagac cgctaaagcg
    21781 tccggaatgg aacgtttccc gctgccgtat gtgctgacca actgccataa ctcactctgc
    21841 gccgtcggcg gcaccattaa cggtgatgac catgtttttg gtttatcggc agcgcagcgt
    21901 tatggcggta tttttgtgcc tccgcatatt gcggtcatcc atcaatatat gcgtgagatg
    21961 atggcaggcg gcggcaaaat gatcctcggg tcagacagcc acacccgtta cggtgcatta
    22021 gggacaatgg cagtcggtga gggtggcggt gagttggtaa aacagctgct taatgacacc
    22081 tgggatatcg actatccggg cgtggttgcg gtgcatctga ccggaaaacc tgcgccgtat
    22141 gtggggccac aggatgtggc gctggctatc attggcgcgg tgttcaaaaa cggttacgtc
    22201 aaaaacaaag tcatggagtt cgttggaccg ggcgttagcg cgctctctac cgatttccgt
    22261 aacagcgttg acgtgatgac cacggaaacc acctgtttaa gctccgtctg gcaaactgat
    22321 gaagaagtgc ataactggct ggcgctgcac ggtcgcggcc aggattactg ccagcttaac
    22381 cctcaaccga tggcgtacta cgatggctgc atcagcgttg atttaagcgc catcaaacca
    22441 atgattgcgc tgccgttcca cccgagcaac gtgtatgaaa tcgacacact gaaccagaac
    22501 ctgaccgaca ttctgcgtga gattgaaatt gagtccgaac gcgtggcgca cggtaaagcc
    22561 aaactctcgc tgctggataa agtggaaaat ggtcgcctga aagtgcagca ggggattatc
    22621 gcgggctgtt ctggcggtaa ctacgaaaac gtcatcgcgg cggcgaatgc actgcgcggt
    22681 caatcctgtg gcaatgacac cttctcgctg gcggtttacc cgtcatcaca gccggtgttt
    22741 atggatttag ccaaaaaagg tgtggtagca gatttgattg gcgcaggcgc aatcatcaga
    22801 accgcgttct gcggcccatg ctttggcgcg ggcgatacgc caatcaacaa cggattaagt
    22861 attcgccaca ccacgcgtaa cttcccgaac cgcgaaggct ctaagccagc taatgggcag
    22921 atgtcagcgg tggcgttgat ggacgctcgt tctatcgctg cgactgcggc aaacggtggc
    22981 tatttaacct ctgccagcga acttgattgc tgggacaacg tgccggagta cgccttcgat
    23041 gtaacgccgt ataaaaaccg tgtttatcag ggctttgtga aaggggcaac tcagcaaccg
    23101 ctgatttacg gaccgaacat taaagactgg ccggaattgg gtgcgctgac tgacaatatc
    23161 gtcctgaaag tgtgctcgaa gatcctcgac gaagtgacca ccaccgacga actgattcct
    23221 tccggtgaaa cctcttctta tcgttcaaat ccgattggtc tggcggagtt taccctgtca
    23281 cgccgcgatc ccggttatgt tggcagaagt aaagcgactg ctgagctgga aaatcagcgt
    23341 ctggcgggga atgtcagcga gctgacagag gtgtttgcgc gcattaagca gattgctggt
    23401 caggagcata ttgatccgct gcaaactgaa attggcagca tggtatatgc ggtgaaacca
    23461 ggcgatggtt ctgcgcgtga acaggcggcg agctgccagc gtgtgattgg cggtctggcg
    23521 aatattgccg aagagtacgc gactaaacgc taccgttcta acgtcatcaa ctgggggatg
    23581 ttaccgctgc agatggcgga agtgccaacc tttgaagtgg gggattacat ttacatccct
    23641 ggcattaaag cggcgctgga taatccgggt acgacgttta aaggttatgt gatccatgaa
    23701 gatgcgccgg taacggaaat tacgctctat atggaaagtc tgactgctga agagcgcgag
    23761 attatcaagg cgggtagttt gattaacttc aataaaaacc gtcagatgta aaaagcgcca
    23821 tgtgaatgta ggtcgcatcc ggtacttatt gtcggatgcg atgcttgcgc atcttatccg
    23881 acctacgaat cgcatcgaat ctgtaggcca gataaggcat ttacgcagca tccggcactt
    23941 attgtcggat gcgatgcttg cgcatcttat ccgacctaca aatcgcatcg aaccgtaggc
    24001 cggataaggc gtttacgccg catccggcaa atagttaatt gctcttactt cttcgcctct
    24061 gcaaccactt tactacccac gccgcggtta ttgtattccc acatgcggtt gtagttagtg
    24121 tcattcagat tgcgctgtat ttcgtcgtga tcatctacgc tgccggtatt acccgcaaac
    24181 ggacgattag agatcaccgc atcggcccac ggtttagccg tgttaaaacc ttcgttgatg
    24241 gcgctatcac ggatcaccac ctgaccgttg gtattggcat caacatccag cgagcggccc
    24301 agttgcgcca cgccatcacc gaaagcattg aaacggctgt ttacggcgag gaaaccgtag
    24361 taaatgttgg acagcgtagc cggtgcaaac acatacgctt cttgctgagt acgtgagttc
    24421 accacgcgga attcggtgtt atcgaacacc actgcgccgc gaccagaaac gatatccaca
    24481 tccccttcaa tgtagctgtt ggtcaccagc gtacgcggct gacgattcgt ttccagacgg
    24541 ttctgcacac cgctgttggt gacaaagaag gtgttctgac gaccgagaat gttaacgttg
    24601 ttaatctgta cctggtcacc atcagtacgc agtgccaccg ccggatggtt acctgcatct
    24661 acgctatcgc ccagcgtgtt ttcgatggtc agattttgca gttgcaggcc attgttttgt
    24721 gaccagaaga ccgcagagca gagaacaccg atactgtcgc tgcgtctgct ctggcagcta
    24781 tcgtacatat accacgctgg cttacctggc atatatttgc cgcgcgggtt gacgtcgtga
    24841 cgccagtcgg cagggctcat gccaccatca agggaaagcc caatcttcac atcaatcggt
    24901 ttttcacctg taccgtacag agtaattcca cccggagcgg cagggacata taccgttccc
    24961 tgatactcac caggcatcac ggcaatatac tggcgcttgt tggtacgctt gataattgcc
    25021 gcatctaccg ccgcctgaat cgtggtatgc gttacacctt gagtgcccgc cgggccgaca
    25081 acaaagtcag gttgcgcagg cagggtaatc ggggaaggat tccacgctgc agcacctggt
    25141 gtcagggatg caaaatagtg ttgagcatcg aaattctgcg cttcttttgc cgacagaatc
    25201 gggcgagaag aggtaccagg cgcggtttga tcagaaggac gttgatcggg cggggttgag
    25261 ctacaggcgg tcagcgtcac gccaaaagcc aatgccagcg ccagacggga aactgaaaat
    25321 gtgttcacag gttgctccgg gctatgaaat agaaaaatga atccgttgaa gcctgctttt
    25381 ttatactaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
    25441 actatcagtc aaaataaaat cattatttga tttcaatttt gtcccactcc ctgcctctgt
    25501 catcacgata ctgtgatgcc atggtgtccg acttatgccc gagaagatgt tgagcaaact
    25561 tatcgcttat ctgcttctca tagagtcttg cagacaaact gcgcaactcg tgaaaggtag
    25621 gcggatcccc ttcgaaggaa agacctgatg cttttcgtgc gcgcataaaa taccttgata
    25681 ctgtgccgga tgaaagcggt tcgcgacgag tagatgcaat tatggtttct ccgccaagaa
    25741 tctctttgca tttatcaagt gtttccttca ttgatattcc gagagcatca acatgcaatg
    25801 ctgttgggat ggcaattttt acgcctgttt tgctttgctc gacataaaga tatccatcta
    25861 cgatatcaga ccacttcatt tcgcataaat caccaactcg ttgcccggta acaacagcca
    25921 gttccattgc aagtctgagc caacatggtg atgattctgc tgcttgataa attttcaggt
    25981 attcgtcagc cgtaagtctt gatctcctta cctctgattt tgctgcgcga gtggcagcga
    26041 cagggtttgt tgttatatgg ccttcagcta ttgcctctcg gaatgcatcg ctcagtgttg
    26101 atctgattaa cttggctgac gccgccttgc cctcgtctat gtatccattg agcattgccg
    26161 caatttcttt tgtggtgatg tcttcaagtg gagcatcagg cagacccctc cttattgctt
    26221 taattttgct catgtaattt atgagtgtct tctgcttgat tcctctgctg gccaggattt
    26281 tttcgtagcg atcaagccat gaatgtaacg taacggaatt atcactgttg attctcgctg
    26341 tcagaggctt gtgtttgtgt cctgaaaata actcaatgtt ggcctgtata gcttcagtga
    26401 ttgcgattcg cctgtctcgg cctaatccaa actctttacc cgtccttggg tccctgtagc
    26461 agtaatatcc attgtttctt atataaaggt tagggggtaa atcccggcgc tcatgacttc
    26521 gccttcttcc catttctgat cctcttcaaa aggctacctg ttactggtcg atttaagtca
    26581 acctttaccg ctgattcgtg gaacagatac tctcttccat ccttaaccgg aggagggaat
    26641 atcctgcatt cgcgcaccca tcgacgaact gtttcaaggc ttcttgggcg tcgctggcga
    26701 gcgttccact cctgaagtgt caagtacatc gcaaagtctc cgcaattaca cgcaagaaaa
    26761 aaccgccatc aggcggcttg gtgttctttc agttcttcaa ttcgaatatt ggttacgtct
    26821 tattcgatgc gcactcctgg tatttcgcct tttgatattg ctaagtcata aatttgcgcg
    26881 gcactatacc catctcgcat ccatgaatct aaggcgcgaa cagcctcgct acgcttttta
    26941 tcttctctct catttttgat atcaacgagg acatcaacgc aattaaggca aatgtggatt
    27001 ttgtctttac attcgatcat ggcggctttg ccatgatttc cgccacacag tgagcataaa
    27061 tcttcagggt ctggctggta tttctgtaac gttagagggt tgaatgttga acaggccata
    27121 atcatctcca taaaacaaaa ctcgccgtag cgagttcaga taaaagaaat ccccgcaagt
    27181 gcgaggatag ttattcattg tcgatattta catttatttc aaatatcttt actgggtaat
    27241 cgccaaagtc atatgttttt tattctgttt tataagcact accaagtact gcttattaca
    27301 aataagaaaa ggctgaaaaa aatatttaag gccacaactg tggccctaaa tttacttacg
    27361 gcactgcgtt aatcagatta caaatacctc ttttggcagg gtctgaattc aacttctgaa
    27421 aattggcgtt caacatggcc gtgtgtaagt gattctcaac ctcaaagaat aggtcgattt
    27481 tgccctcttt ggctgcctgt ttcttccaaa tattcacttg gtcatcagca atacgactgg
    27541 cgcaagtgaa caagaacgaa ctgcctctag gaatggatgt cgtgctgtaa tactcaccat
    27601 ctacttctac atatttagct cggaatgact ctaaggttag ctcattgccg ttcttgaccg
    27661 acttagcttc tgccttactt gtttcttcct gctcttcaga atgtttctgc gccgacgtgg
    27721 gtatgtcatt ggccgctgtt acgctgactg aactccccaa acctttgagg ccattaattt
    27781 ggataagata agccagctcc agagccttat tatcagtgag ttttccacgc atatttaccc
    27841 actgacgttg aaccattttt agggtgtctg cgttctctgg gtttcctttg agggcactag
    27901 aataggcaga gctaagctgt tcgtctaatt ttgacagacg ttcgttatcg cagattttat
    27961 gttcgattgc tgtcgaagcc ttttggcagt caaagctggc tgcaaatgca cccggcgata
    28021 ccactaacaa tgatgccagt aggatatttt tcatattcac tccataaata caaaagcgca
    28081 atactatagc acttaaccga tcgcttatct cgcccgttcg tcattcttat ctaaccagaa
    28141 catgcgactg ccacagcaca tgtcgaggat tgctgcatgt ccagtcactg gttgcctcct
    28201 ttgcgaagct ggtcggcgaa caaacgtaca cca
//
LOCUS       DAZKHC020000060        27099 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZKHC020000060 DAZKHC020000000
VERSION     DAZKHC020000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.060"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            68..1666
                     /gene="rtcR"
                     /locus_tag="ACHOSM_RS14610"
                     /old_locus_tag="pgaptmp_002922"
     CDS             68..1666
                     /gene="rtcR"
                     /locus_tag="ACHOSM_RS14610"
                     /old_locus_tag="pgaptmp_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HGU4559405.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1648..2406)
                     /locus_tag="ACHOSM_RS14615"
                     /old_locus_tag="pgaptmp_002923"
     CDS             complement(1648..2406)
                     /locus_tag="ACHOSM_RS14615"
                     /old_locus_tag="pgaptmp_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HGU4559406.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2423..3253)
                     /gene="glpG"
                     /locus_tag="ACHOSM_RS14620"
                     /old_locus_tag="pgaptmp_002924"
     CDS             complement(2423..3253)
                     /gene="glpG"
                     /locus_tag="ACHOSM_RS14620"
                     /old_locus_tag="pgaptmp_002924"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HGU4559407.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3298..3624)
                     /gene="glpE"
                     /locus_tag="ACHOSM_RS14625"
                     /old_locus_tag="pgaptmp_002925"
     CDS             complement(3298..3624)
                     /gene="glpE"
                     /locus_tag="ACHOSM_RS14625"
                     /old_locus_tag="pgaptmp_002925"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HGU4559408.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3814..5319
                     /gene="glpD"
                     /locus_tag="ACHOSM_RS14630"
                     /old_locus_tag="pgaptmp_002926"
     CDS             3814..5319
                     /gene="glpD"
                     /locus_tag="ACHOSM_RS14630"
                     /old_locus_tag="pgaptmp_002926"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HGU4559409.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5371..5976)
                     /locus_tag="ACHOSM_RS14635"
                     /old_locus_tag="pgaptmp_002927"
     CDS             complement(5371..5976)
                     /locus_tag="ACHOSM_RS14635"
                     /old_locus_tag="pgaptmp_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559410.1"
                     /translation="MIRFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5979..6746)
                     /locus_tag="ACHOSM_RS14640"
                     /old_locus_tag="pgaptmp_002928"
     CDS             complement(5979..6746)
                     /locus_tag="ACHOSM_RS14640"
                     /old_locus_tag="pgaptmp_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559411.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERPILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6749..7459)
                     /locus_tag="ACHOSM_RS14645"
                     /old_locus_tag="pgaptmp_002929"
     CDS             complement(6749..7459)
                     /locus_tag="ACHOSM_RS14645"
                     /old_locus_tag="pgaptmp_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559412.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7475..8983)
                     /locus_tag="ACHOSM_RS14650"
                     /old_locus_tag="pgaptmp_002930"
     CDS             complement(7475..8983)
                     /locus_tag="ACHOSM_RS14650"
                     /old_locus_tag="pgaptmp_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU4559413.1"
                     /translation="MMQNRKWILSSLVMTFFGIPILTQFLATVVAMLGVGLAGIIEVC
                     NILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLIL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLE
                     SSRTPDAYNRIVKGDADIIFVAQPSGEQKKRAEESGITLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIIDTFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(9109..11556)
                     /gene="glgP"
                     /locus_tag="ACHOSM_RS14655"
                     /old_locus_tag="pgaptmp_002931"
     CDS             complement(9109..11556)
                     /gene="glgP"
                     /locus_tag="ACHOSM_RS14655"
                     /old_locus_tag="pgaptmp_002931"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HGU4559414.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11575..13008)
                     /gene="glgA"
                     /locus_tag="ACHOSM_RS14660"
                     /old_locus_tag="pgaptmp_002932"
     CDS             complement(11575..13008)
                     /gene="glgA"
                     /locus_tag="ACHOSM_RS14660"
                     /old_locus_tag="pgaptmp_002932"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGU4559415.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(13008..14303)
                     /gene="glgC"
                     /locus_tag="ACHOSM_RS14665"
                     /old_locus_tag="pgaptmp_002933"
     CDS             complement(13008..14303)
                     /gene="glgC"
                     /locus_tag="ACHOSM_RS14665"
                     /old_locus_tag="pgaptmp_002933"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGU4559416.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14321..16294)
                     /gene="glgX"
                     /locus_tag="ACHOSM_RS14670"
                     /old_locus_tag="pgaptmp_002934"
     CDS             complement(14321..16294)
                     /gene="glgX"
                     /locus_tag="ACHOSM_RS14670"
                     /old_locus_tag="pgaptmp_002934"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HGU4559417.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASELRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16291..18477)
                     /gene="glgB"
                     /locus_tag="ACHOSM_RS14675"
                     /old_locus_tag="pgaptmp_002935"
     CDS             complement(16291..18477)
                     /gene="glgB"
                     /locus_tag="ACHOSM_RS14675"
                     /old_locus_tag="pgaptmp_002935"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HGU4559418.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRISVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEECKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18750..19853)
                     /gene="asd"
                     /locus_tag="ACHOSM_RS14680"
                     /old_locus_tag="pgaptmp_002936"
     CDS             complement(18750..19853)
                     /gene="asd"
                     /locus_tag="ACHOSM_RS14680"
                     /old_locus_tag="pgaptmp_002936"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU4559419.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIQVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            20045..20638
                     /gene="yhgN"
                     /locus_tag="ACHOSM_RS14685"
                     /old_locus_tag="pgaptmp_002937"
     CDS             20045..20638
                     /gene="yhgN"
                     /locus_tag="ACHOSM_RS14685"
                     /old_locus_tag="pgaptmp_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HGU4559420.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLITIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20695..22035)
                     /gene="gntU"
                     /locus_tag="ACHOSM_RS14690"
                     /old_locus_tag="pgaptmp_002938"
     CDS             complement(20695..22035)
                     /gene="gntU"
                     /locus_tag="ACHOSM_RS14690"
                     /old_locus_tag="pgaptmp_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU4559421.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(22039..22566)
                     /gene="gntK"
                     /locus_tag="ACHOSM_RS14695"
                     /old_locus_tag="pgaptmp_002939"
     CDS             complement(22039..22566)
                     /gene="gntK"
                     /locus_tag="ACHOSM_RS14695"
                     /old_locus_tag="pgaptmp_002939"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HGU4559422.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(22705..23700)
                     /gene="gntR"
                     /locus_tag="ACHOSM_RS14700"
                     /old_locus_tag="pgaptmp_002940"
     CDS             complement(22705..23700)
                     /gene="gntR"
                     /locus_tag="ACHOSM_RS14700"
                     /old_locus_tag="pgaptmp_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HGU4559423.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(23924..24619)
                     /gene="yhhW"
                     /locus_tag="ACHOSM_RS14705"
                     /old_locus_tag="pgaptmp_002941"
     CDS             complement(23924..24619)
                     /gene="yhhW"
                     /locus_tag="ACHOSM_RS14705"
                     /old_locus_tag="pgaptmp_002941"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HGU4559424.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(24742..25779)
                     /gene="yhhX"
                     /locus_tag="ACHOSM_RS14710"
                     /old_locus_tag="pgaptmp_002942"
     CDS             complement(24742..25779)
                     /gene="yhhX"
                     /locus_tag="ACHOSM_RS14710"
                     /old_locus_tag="pgaptmp_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU4559425.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLEEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            26112..26600
                     /gene="yhhY"
                     /locus_tag="ACHOSM_RS14715"
                     /old_locus_tag="pgaptmp_002943"
     CDS             26112..26600
                     /gene="yhhY"
                     /locus_tag="ACHOSM_RS14715"
                     /old_locus_tag="pgaptmp_002943"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU4559426.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEETGKKYALRNGEYVDAYYMAR
                     VK"
     gene            26837..>27099
                     /gene="tssD"
                     /locus_tag="ACHOSM_RS14720"
                     /old_locus_tag="pgaptmp_002944"
     CDS             26837..>27099
                     /gene="tssD"
                     /locus_tag="ACHOSM_RS14720"
                     /old_locus_tag="pgaptmp_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HGU4559427.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGF"
BASE COUNT         6814 a         7194 c         6713 g         6378 t
ORIGIN      
        1 aaacggagat tttccacaaa accaattcac cgataatgaa atatcgtctt ttataaggat
       61 atctaagatg cgtaaaacag tggcttttgg ctttgtcggt accgtgctgg attatgccgg
      121 acgcggcagt cagcgctggt cgaaatggcg tccgacgctt tgtttatgcc agcaagaatc
      181 gttagtcatc gatcggctgg aattgttgca cgacacccgc tcgcgctcgc tattcgaaac
      241 gcttaaacgc gatatcgcca gcgtttcgcc agaaacagaa gtcgtgggag ttgagattga
      301 actgcataac ccgtgggatt tcgaagaggt ttacgcctgc ctgcatgatt tcgcccgtgg
      361 ttacgagttt cagccagaaa aagaagacta tttaattcac atcaccaccg gcacccacgt
      421 cgcgcagatt tgctggtttc tgctggcgga agcgcgttac ctgcccgccc ggctgataca
      481 atcttcacct ccgcgcaaaa aagaacagcc gcgcggcgca ggtgaagtga cgattatcga
      541 tctcgattta agccgttata acgccatcgc cagccgcttt gccgaggaac gccagcaaac
      601 gcttgatttt cttaagtccg gcattgccac gcgtaacccc cacttcaacc gcatgattga
      661 gcagatcgaa aaagtggcga tcaaatcccg cgcgccgatt ctgcttaacg gtccaaccgg
      721 cgcgggcaag tcatttctgg cgcgacgcat cttcgagtta aaacaggcgc ggcatcagtt
      781 tagcggcgcg tttgtggaag tgaactgcgc caccctgcgc ggcgataccg ccatgtcgac
      841 gctgtttggt catgtaaaag gcgcgtttac cggggcgcgg gaatctcgtg aaggtttatt
      901 acgcagcgcc aacggcggaa tgttgtttct tgatgagatt ggcgaactgg gcgcagacga
      961 acaagcaatg ctgctaaaag ccattgaaga gaaaaccttt tacccgtttg gcagcgatcg
     1021 ccaggtgagc agtgattttc agcttatcgc cggaacggtg cgcgatttgc gccagctggt
     1081 tgccgaaggc aaatttcgcg aagatctgta cgcgcggatc aatctctgga ccttcaccct
     1141 gccgggtcta cgccagcgcc aggaagatat tgaaccgaac cttgattatg aagtggagcg
     1201 ccacgccaca ctcactggcg acagcgtgcg ttttaacacc gaagcgcggc gcgcctggtt
     1261 ggcttttgcg acctctcccc aggcaacatg gcgcggtaac tttcgcgaac tttctgccag
     1321 cgtcacgcgg atggccacct ttgccactag cggacgcatc actctggacg tggttgaaga
     1381 tgagataaac cgtctgcgct ataactggca ggagagccgc ccctccgcgc ttacggcgtt
     1441 gctgggcgca gaggcagaaa acatcgacct ttttgacagg ctgcaactgg aacacgtgat
     1501 agctctctgt cgccaggcaa agtcgctttc cgctgccgga cgcctgcttt ttgatgtttc
     1561 gcgccagggc aaagccagcg tcaatgacgc ggatcggcta cgcaaatacc tggcgcgttt
     1621 tggtctgacg tgggaagccg tgcaggatca gcacagctcc agttgaatat gatggtccgt
     1681 cagcacctgc attacgctta ctggcggcgg ggcgtcggtg tagacggcat ctaccatgct
     1741 gatgctgccc atattgacca tcgcgttacg gccaaatttc gagtgatcga caaccagcat
     1801 aacgtggcgc gagttctcaa taatggcgcg cttggtgcga acttcgtggt aatcgaactc
     1861 cagcagcgag ccgtcgctat cgatgccgct tatccccaga atgccgaaat caaggcggaa
     1921 ctgggagata aaatcgagcg tcgcttcgcc aatgatcccg ccatcgcggc tgcgtaattc
     1981 gccaccggcg agaataatgc ggaaatcttc ttttaccatc aacgtgttag caacattgag
     2041 attgttggtg acaatacgca aattgctgtg attaagcagc gcgtgcgcca ccgcttccgg
     2101 cgtggtgccg atatcgataa acagtgtcga gccgttgggg atttgctccg ccactttgcg
     2161 ggcaatcctc tctttttctt cggtctgggt ggctttgcga tcgtgccacg gcgtgttaac
     2221 cgaactggaa ggcagcgccg caccgccatg gtgacgcagg atcaggtttt gctccgccag
     2281 ctcattgagg tcgcggcgaa tagtctgcgg gctgacggag aaatgctcta ccagctcttc
     2341 agtactgaca taaccctgct gtttaaccag ttcgataata ccgttgtgac gttgtgtttg
     2401 tttcatttat aaatccctgg aattattttc gttttcgcgc attgagcgaa tcaacaaaag
     2461 ccatcgctaa ccccacggct aacccggcga tgtgtgctcc gttcgccatc gacatcccaa
     2521 acaaatcaaa ccatccggcg acaatccaga tcagcgcaaa gataattaac ccacgttgca
     2581 ggtaaatgcc actttgcgga tcgcgttcac cacgtagcca gacgtagccc atcagcgcat
     2641 acaccacgcc agaaagcccg ccaaaccacg gcccgctgaa tttttgctgc acatagccgc
     2701 ttaacagggc gctaatgaga gtaataacaa ttagcttacc gctaccgagg cgtttttcca
     2761 ccgcaccgcc gagataccac caccagagca ggttaaagag gatatgcatc agcgagaagt
     2821 gcattaacgc gtgggtgaag taacgccaga actcaaattt cagtgttgga tcgaatggcc
     2881 aggccagcca taacatcact tcctgatcgc cgagaatttg catggcgata aacaccacca
     2941 cgcaggcgat catcaccacc caggttaccg gacctgcgcg ttcacgcaag gcagcaaaga
     3001 aaggataacg gcgataatgc agaccactgc cggtatggcc cgcctgccag ctggccgcca
     3061 gataacgcgg atctgccggg ttttcgagaa aacgcgccag ctccgcccgc acgcgctcgg
     3121 cctgggactc atccgccagc cagacatcgc tttggttatg ttgttgaatc gtaaggataa
     3181 caccctgcgt cgccatgtaa tcaacaaacg cctgcgccac gcgggggtta gcaaaagagg
     3241 taatcatcaa catcgttgct gtcgcttatt ccacacaaaa ggggacagta taaagcgtta
     3301 cgcgccgtac gccacctctg cgggaaactg acgctgccag gcttcaaaac cgccgtcaat
     3361 gctatagacc acatcgtagc cctgttgcag cagatactgc gccgcgcctt tgctgctatt
     3421 gccgtgataa cacatcacca tcaccggagt gtcaaagtcg ttatcacgca taaacgcgcc
     3481 cagcgtgtcg ttggttaaat ggaaagcctg caccgcatgt cccattgcga aactctgtgg
     3541 atcgcgaata tcgaccagca ccgcctcttt ttcctgcaac ttctggtgcg cgtcggcaac
     3601 gttaatacat tcgaacagat ccatgcgtct ctctttcttt acaaacaagt gggcaaattt
     3661 accgcacagt ttacgtcgaa gcggcagata aacgccataa tgttatacat atcactctaa
     3721 aatgtttttt caatgttacc taaagcgcga ttctttgcta atatgttcga tagcgaacat
     3781 ttatgagctt taacgaaagt gaatgagggc agcatggaaa ccaaagatct gattgtgata
     3841 ggtggcggca tcaatggtgc tggtatcgcg gcagacgccg ctggacgcgg tttatccgtg
     3901 ctgatgctgg aggcgcagga tctcgcttgt gcgacctctt ccgccagttc aaaactcatt
     3961 cacggtggcc tgcgctacct tgagcactat gaattccgcc tggtcagcga ggcgctggct
     4021 gaacgtgaag tgctgctgaa aatggctccg catatcgcct tcccgatgcg ttttcgcctg
     4081 ccacatcgtc cgcatctgcg cccggcgtgg atgattcgga ttggtctgtt tatgtacgat
     4141 catctgggta aacgcaccag cctgccggga tcaaccggtt tgcgttttgg cgcaaactca
     4201 gtgttaaaac cggaaattaa gcgcggattc gaatattctg actgttgggt agacgacgcc
     4261 cgtctggtac tcgccaacgc ccagatggtg gtgcgtaaag gcggcgaagt gcttactcgg
     4321 actcgcgcca cctctgctcg ccgcgaaaac ggcctgtgga ttgtggaagc ggaagatatc
     4381 gataccggca aaaaatatag ctggcaagcg cgcggcctgg ttaacgccac cggcccgtgg
     4441 gtgaaacagt tcttcgacga ggggatgcat ctgccttcgc cttatggcat tcgcctgatc
     4501 aaaggcagcc atattgtggt gccgcgtgtg catacccaga agcaggccta cattctgcaa
     4561 aacgaagata aacgtattgt gttcgtgatc ccgtggatgg acgaattttc catcatcggc
     4621 accaccgacg tagagtataa aggcgatccg aaagcggtga agattgaaga gagtgaaatc
     4681 aactatctgc tgaaagtgta taacgcgcac tttaaaaaac agcttagccg tgacgatatc
     4741 gtctggacct actccggtgt gcgtccgctg tgtgatgatg agtccgactc gccgcaggct
     4801 attacccgtg attacaccct tgatatccat gatgaaaacg gcaaagcacc gctgctgtcg
     4861 gtattcggcg gtaagctgac cacctaccga aaactggcag aacatgcgct ggaaaaactg
     4921 acgccgtatt atcagggtat tggcccggcg tggacgaaag agagcgtgct gccgggtggt
     4981 gccattgaag gcgaccgcga cgattacgca gctcgtctgc gccgtcgcta tccattcctg
     5041 actgaatcgc tggcgcgtca ttacgcacgc acttacggca gcaacagcga gctgctgctc
     5101 ggcaatgcgg gggcgataag cgacctcggg gaggatttcg gtcatgagtt ctacgaagcg
     5161 gaactgaaat acctggtgga tcacgaatgg gtccgccgca ccgacgatgc cctttggcgt
     5221 cgcacaaaac aaggcatgtg gctgaatgcg gatcaacaat ctcgtgtgag tcagtggctg
     5281 gtggaatata cgcagcagaa gttgtcgctg gcgtcgtaag gatgatgccc ggttgaagtg
     5341 caaccgggct atttggattc cttagactat tcattgtaaa taattgaata acggcccctg
     5401 agctaaaatc gccaaactta agcacgttac ccaatcgccc cacttcttcc tctggtttct
     5461 attttttctg caccaatggc aataatatcc caatgcaaaa ccaagctgtg cactaatcgg
     5521 gccgacaaaa tcagtccaag gctgcggcca aaaaggggaa aaattcaggg gaggccagac
     5581 aaacgtcaga aagaaatttc ggatattttc cagcaacgct gatttctctg gagaaatata
     5641 aagaatgaaa ttcgagagaa ttaaccagaa atagaccggc gaaaagacca gccagcaatg
     5701 cagtctgttt tttaattcag gaggcgttct gcgggagaat tgccaaccga tgccagccat
     5761 tacactgaaa aaaagtgcat attcaaccat taactcaagt tgtaattcct gaagagaaat
     5821 acgcacaacc aggagcggaa aggagaatat tatttctgcc agaaaagtta acgtgaatat
     5881 aagtacggct aagacaataa ccgtcggcca taccaataat ggcacgataa caaaaccgag
     5941 aattacaggg ggaatataaa gccggaatct aatcattttt attttcctgg tgattcggat
     6001 gatgcgtcat acatcgcgca taaacactca tcaattatcc agtcatcttc ccagacctga
     6061 tattctgtca ttggttcggg ctgttttagc gggttctcaa tcgcttgccg cgctctttct
     6121 ttcagtaaag gatgattatt tctagcccag gcattgattt gggtggtaat gacgttatcg
     6181 atatcgtcct ggttatctct gatataatcc tcacaatatc tgttcaataa acaggcaaga
     6241 gaaacggtcg accaaaagcc gggaactgaa ttaatggcat ccagcaatgg cgcaagagcg
     6301 atatcggcac ggaaaaacat ttccagcata tactgtaatt cgacagcttc atcgccggat
     6361 acccaatcag tcatatactg gcaggcaaat cgctgtaaaa ttggccgttc atccgcacgc
     6421 cagttttctt tgttggcaag gttgatacgg cgcagagaaa aaactacgtg aatattgtca
     6481 aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa aatagcggac ctcatcgcta
     6541 ttttgcggat caggccctgg gctactattc caggcattga ttaaggaata cgggatcgct
     6601 cgtaacgatg tactccgtaa tgccttttgt tcctgctgac tcaggcaata ttcacaacaa
     6661 accacaaatt tctgcggcac ccgataacga cgaaaggtgt gataaatctg ctcaattaat
     6721 gcctgcatgg cggggctggg ttgcatagtt accccctcgc ccgctgatcg cgtgtaaacg
     6781 gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat aagggcgcta cgcgaaaggg
     6841 aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct ttcctctgcg caattttcca
     6901 ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt atcgcgaaat ccgcgtaaac
     6961 tgttattgtt atgaaatttc caggcatctt taaagacctg aaagatatag acatcctcaa
     7021 tccaggagtc acatttttta agcgggcgct ttgctattat cacccgctca tccggattta
     7081 gcatcgccgt aaattgtacg gtgacgtcat ttgtcgtgtt caggtcacat tgaacatctg
     7141 gtacatcact gatatagggg tcaaatccaa aatcagcacc acaggcacaa agacggctaa
     7201 tggtggcggt atcgagggaa aatcccggcc atactttgct ggaggttaag ccaatattta
     7261 atgtgacatc atatcgaagc aatccctcaa gaccgtcagc accgacgaac ttttcccctt
     7321 catccagggc ctggttaata agttgctgaa caatgccttc atcccagtat gccgatttgc
     7381 agttggcaat cgtaatatcc acgcctgtga agggatcagt accggcaagg cttgcaaata
     7441 atgtattaac taattccatt tttattatcc agctttaagg tagcgtctta tacatcggga
     7501 cataacctac atcttccacc aaactctgtc cctgcggcgt taaaaaccac tccaacaatt
     7561 tttgtgtctc tgacgtcgta ttttcccgcg ttaccataaa ggtatcgatg atatagggat
     7621 atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc gttaatcgct agcagtttta
     7681 tatttttatc ggcgttcatt tgtgttgcgt aataacggaa ggtgtaacct attgcgttgt
     7741 ttgtattacg gtattcggca acgactttaa tcattccctc catcatactt gccacttctg
     7801 tttcctgcgg cgagatcatg cggacatttt tcatgacctg ggattgcatc accgtctggc
     7861 taccagagtc ttccgggcgt tgccaggtct ggatctcctg atcgttaccg ccaacagtac
     7921 gccagttggt aattgcacca ctgaagatgt cacgcacctg ttgttcagtc agggaattaa
     7981 ccggattatc cgcattgacg atgaaaacaa acgcttcacg ggcaaaaggc gtgtacagca
     8041 aagtaatgcc cgattcctca gcgcgttttt tctgctcacc ggaaggttgc gccacaaaaa
     8101 taatatcagc atcaccttta acaatcctgt tatacgcatc gggggtacga gagctctcca
     8161 gatattccct ggtgtgaaaa tcttccggta taacgcttaa agcataaaat gcagaggcat
     8221 aaattgggta cgccgccgta gcaccatcga gtcgcggcca gttttgcgta aactgaattt
     8281 gcggtttacc acgcagtggt gtcagttgat tattgagttt atcgggtcgc caggcccagg
     8341 tatcaatatt ttcgcgcaca ccgacgccgg gattgagctg tttatataaa taaccctgat
     8401 aaccagcgac aaaaccaagc atgagaagga taaacaggag ggaattacgg caacgtagcc
     8461 cggatgtacc ggttaaagga tgtctggttg gccggccgta ccccagcgaa aaactgatat
     8521 gcgcgccaac gggtataatg actatccacc agtactcacc tccgactact gagactaaca
     8581 aaacgcccag ccaggggagc ataagatcag ccaaccaacc cgatcccatc tggtgaaatt
     8641 gataatctgc cagatgcagc gcgattatcc agccaaccag gattaataaa ccgggtacta
     8701 aaaaaagaca ttgtcgccag acggctattt ctcttttttc tggtgcacta tcgcctgccc
     8761 atactcgccc cgagaaaaat agcattaatg cgcccagcgc cagcataaaa atgttgagca
     8821 gaaggtaact tgttggcgtg ataaggatat tacaaacttc aataataccg gcaagtccga
     8881 cacccagcat agcaacaacc gtcgccaaaa attgcgtcag tatggggatg ccgaaaaaag
     8941 tcattaccag cgaggacaaa atccattttc tgttttgcat tattctttcc attctttttg
     9001 aatggtgaaa ttatactccc cgagtcccct tgccccttct ggacactttt ccgaaatgat
     9061 ggcggaaaaa aacgggaccc tttggccccg ttctatttat tggtgaactt acaatctcac
     9121 cggatcgata tgccagatat gatcggcgta ctctttgata gtacggtcag aagagaagta
     9181 gcccatattg gcaatgttca gcatcgcttt tgcggtccac tcttcctgaa gctcgtagag
     9241 ttcgtcgact ttatcctgac aatcgacata gctgcgataa tccgccagaa cctgatagtg
     9301 atcgccgaag ttgatcagcg aatcaaccag atcgcgatag cgacccggat cttccggact
     9361 gaatacaccg ctgccgattt gcgtcagcac ctgatgcagc tcctcatctt tctcgtagta
     9421 ttcacgcggt ttgtagccct gacgacgcag ttcttccact tcttccgctg tgttaccaaa
     9481 aataaagata ttgtcagcac cgacatgatc cagcatctcg acattcgcac cgtccaacgt
     9541 accgatagtc agcgcaccgt taagcgcaaa cttcatgtta ctggtgccgg aagcttccgt
     9601 ccctgccagc gaaatctgtt cagacagatc tgccgccgga atgatcaact gcgccaggct
     9661 aacgctgtag ttcgggatga acacgacttt cagcttatcg ccaatctgcg gatcgttgtt
     9721 gatcactttc gctacgtcat tgatcaaatg aataatgtgc ttcgccatgt aataggccga
     9781 agccgcctta ccgccaaaaa tattcacgcg cggtacccac ttcgcatccg ggtcggcctt
     9841 gatgcggtta tagcgggtaa tcacatgcaa cacattcatc aattgacgtt tgtattcgtg
     9901 aatacgtttg atttgtacat cgaacaacgc ctttggattc accaccacat tcagctgctg
     9961 ggcgatatac tctgccagac gctttttgtt ctccagcttc gcctgatgca cagcgtgatt
    10021 aaccattggg aaatcacagt gttgttgcag ctcattaagc aggctaaggt cggtgcgcca
    10081 gttacggccc aggtgttcgt ccagcacggc tgaaagcgat gggttcgcta ccgctagcca
    10141 gcgacgcggc gtcacaccgt tggtgacgtt ggtgaaacga cccgggaaga ttttcgcaaa
    10201 gtcggcaaac aacgattgca ccatcagatt agagtgcagt tccgatacac cgttaacttt
    10261 gtggctcaca acaaccgcca gccaggccat acgcacacga cgaccgttgg attcatcaat
    10321 gatcgacgcc cgtcccagca gatcggtatc gttcggatac tgttcctgca aggttttcag
    10381 gaaatagtcg ttgatttcaa agatgatctg caggtgacgc ggcagaattt tacccagcat
    10441 atcaaccggc caggtttcca gcgcctcgct catcagcgtg tggttagtgt aggagaagac
    10501 ctgacaacac acctcaaacg cgtcgtccca gctaaattgg tgctcatcga tcagcagacg
    10561 catcatctca ggaatcgaca gtaccggatg ggtatcattg agatgaatcg cgattttatc
    10621 cgccaggtta tcgtaggttt tatgcaactg ataatggcgg cttaaaatgt cctgaatggt
    10681 cgaggaaacc agaaagtatt cctgacgcag gcgcagctca cgcccggagt aggtggagtc
    10741 atccggatac agtacgcgag atacgttctc ggagtggttt ttatcttcca ctgccgcgaa
    10801 gtagtcaccc tggttgaatt taccgaggtt aatttcgcta ctggcttgcg cactccacaa
    10861 acgcaacgtg ttggtcgcgt cggtgtcgta accagggatt atctgatcgt aagcgactcc
    10921 cagaatctct tcggtttcaa tccagcgcgt ttttttacct tcctgctgaa tgcgaccacc
    10981 aaaacggact ttatagcgcg tgttgtggcg tttgaattcc cacgggttac cgtattccag
    11041 ccagtagtct ggcgactctt tctggctacc gttaacgatg ttctgcttga acataccgta
    11101 gtcatagcgg atgccgtaac cgcgccccgg caaccctaac gtcgccagag aatcaaggaa
    11161 gcaagccgcc agacgtccca ggccaccgtt accgaggcct gggtcatttt cttcatcaat
    11221 cagctcttcg agatttaacc ccatcgcttc cagtgcgccc tgtacatctt cgtaaattcc
    11281 tagcgacaac atggcgttgg agagcgtacg gccaatcaaa aactccatcg acaggtagta
    11341 aacctgacga gtttcttgcg acaactgggc acggtttgaa cgtaaccagc gctccacgag
    11401 acgatcgcgc acagcaaata acgttgcgtt cagccattca tgtttattgg cgacgaccgg
    11461 gtcctttcca atcgtaaaca tcagcttgta agcgatagag tgcttaagag cttctacgct
    11521 aagcgtgggc gatgaatatg taaacggagc attcatatag gcgtttcctg aaaactattt
    11581 caagcgatag taaagctcac ggtacgactt cgccgcgacc tgccagctaa aatccattgc
    11641 catagcctga cgttgcacaa accgccacag tgaaggacgg gaccacagta caaaagcacg
    11701 tcgaatagcc cgtaacagcg accaggcatt actatcttca aagacaaacc cactggcgac
    11761 gccatctgca aggttctcaa gagaacagtc agaaaccgta tcagcaagcc caccggtgcg
    11821 ccgcactaac ggcagcgtac cgtacttcaa tccataaagt tgcgttaagc cgcacggttc
    11881 aaaacggctg ggcaccagaa tgacgtccgc gccgcccata atgcgatgcg aaaatgcttc
    11941 gtgatagcca atctgaacgc ccacctggcc ggggtattcc gctgccgccg caaggaaacc
    12001 ttcctgcagc accggatcgc ccgcgccgag tagcgccagc tgcccgccct gctccagaag
    12061 acccggtaag gcttccagca ccaggtcgag acctttctgg ctggtcagac ggctcaccac
    12121 tgcaaaaagc ggcactttat cgtcaacctt aagccccatt gcgatttgta actggcgctt
    12181 attttccgct ttatcttcca acgtatcgcg ggtgtaacgc gaggccaaca gtaagtccgt
    12241 ctctggactc cagattttct cgtccacgcc gttcagtacg ccggaaagac gcccttcacg
    12301 gtgacgctgt tgcaacagac cttccatacc gtaggcaaac tgcggttcgg tgatctcgcg
    12361 agcgtaggtt ggactgaccg ccgtaatgtg atcggcatag tacagaccgg ccttcaggaa
    12421 agagatttgt ccgttgaatt ccagcccatg aatattaaag aatgaccatg gcaattggat
    12481 gtcattcatg tgatgtgcat aaaacatgcc ttgataggcc aagttgtgca cagtaaacac
    12541 cgacttcgcc ggacgcccgc gcgccgccag atacgcaggc gcaaggcctg catgccagtc
    12601 gtgcgcatgc accacatcag gacgccagaa tgggtcaagc ccgctggcca tttctgcccc
    12661 aacccacccc agcagcgcaa aacgcaatac gttgtcggta taggcaaata agttggtatc
    12721 gtgatacggg cttcccggac gatcatagag atgcggcgcg tcaatcaggt atatgccaac
    12781 cccgttgtaa tgaccgaaca acagcgtgat atgtccggcg aaggtatcac gacgggatac
    12841 tacctgcgca tcggtcacgc cacggcgaat atcgggaaat gcaggcaaca gtacgcgagc
    12901 gtcaacgccg tctgcgattt gtgctgcggg taatgcccca ataacatcag ccagaccgcc
    12961 ggttttaagc agcgggaaca tctctgaaca tacatgtaaa acctgcatta tcgctcctgt
    13021 ttatgcccta acttccgtag catttcgcgc gttaccagca cgatgccttc ttctgaacga
    13081 tagaaacgac gtgcatcttc ctctgcgttt tcaccaatca ccatgccttc cgggataaca
    13141 caagcacgat cgatgacgca gcggcgcaga cggcacgagc gacctaccca tacttccggt
    13201 aacaatacgg cggaatcaat gttgcagaat gaattcacgc gaacgcgcga gaacagaacg
    13261 gactgcacca ccaccgaacc ggagatcaca caaccgccgg ataccagtga gttaagggtc
    13321 atcccgtggc taccggagcg atcctgcacg aatttcgctg gcggtaatga ttcattgtag
    13381 gtgcgaattg gccaattgcg atcgtacata tccagttccg gcaccacaga ggccagatcg
    13441 aggttcgctt tccagtaagc ttccagcgta cccacatcgc gccagtacgg ctcggcatcc
    13501 gggtcggatt gtacgcaaga gagcgggaac gggtgcgcat aggccagacc ggcttcggtg
    13561 atcttgggaa tcaaatcttt gccaaagtcg tggctggagt tctcatcacg atcgtcttct
    13621 tccagcagtt catacagata gtcggcgtca aagacgtaga tacccatact cgccagagat
    13681 ttgctcggat cgttcggcat tgacggtggg ttagcaggtt tttcaacgaa ttcgataatt
    13741 ttatcgttct catcaaccgc cataacgcca aatgcggagg cttcttcaat cggtactggc
    13801 atacaagcaa cggtgcaacg tgcgcctttt tcgacgtgat cgataagcat acgcgagtag
    13861 tcttgcttgt agatatggtc gcccgccagg atcaccacgt attccgcttt ataacggcgg
    13921 ataatgtcga ggttttgggt gaccgcatct gcggtgccgc gataccagtt ttcccctttc
    13981 attctctgct gtgctggcag cagatcgaca aactcgttca tttcttcatt gaagaatgac
    14041 cagccgcgct gaatgtgctg caccagagtg tgggactggt actgggtgat cacgcccata
    14101 cgacggatcc cagagttgat gcagttagac agcgcaaagt caataatgcg gaacttaccg
    14161 ccgaagtgta cggccggttt tgctcgctta ttggttaaat ccttcaggcg ggtaccacgt
    14221 cctcccgcca gtatcagggc aacagatttc aatggcagct ggcgcgccaa cattaagtga
    14281 tcgttcttct ctaaactaac catgactaac tcctttttta tcatctctgg aacacacaca
    14341 atccgtgtgc aggtccctgc cagacagccg taatcactgg gttatcctct ccagcgaatg
    14401 ggggaatggc gtgccactcc ccagcaggta aaacaatctc tgttacctca agcgtggcgt
    14461 taattgcgat caaaaagcga tccgagagca gaatttgcag ctgtttcggc ccgttttgcc
    14521 actcatccgt gcttaaaggt tgagcatatc gatttagcca acggacattg ccgtcgcctt
    14581 cttcccacca gcgattctcc accaaagcgg gaatgcgctt gcgcagatgg attaacgcgg
    14641 cggtaaatgc ggttaaacca ctgcttgcct gcgaccagtc caaccaggtt aattggttat
    14701 cctgacagta ggcattgtta ttgccatgct ggctgtgacc atgttcgtca ccggccagta
    14761 acatcggcgt gccctgggag agcaacaacg ttgttaacag ggcatgaatg ctgtcgcgcc
    14821 gccgttcaac caggtcaaga gtaccgccta acccttcttt accatgattg ttactgtaat
    14881 tgttgttggt cccgtcgcga ttttcttctc cgtttgcttc attgtgttta tggttgaagc
    14941 aaacgcagtc gcgaagcgta aaaccgtcat gcgcggtgac gagattaatc gcggcactcg
    15001 gcagacgacc attacgttta aaaacatcgc tggaggcagc aaaacgcccg gcaaacgccc
    15061 ccagaggcaa atcataatgt agccagaaac gacgggcagc atcgcggaaa tgatcgttcc
    15121 actcggcaaa cagcggcggg aaatttccca cctgataacc accaggagcg atatcccacg
    15181 gttcagcaat taacttcacc tgcgagagca ccgggcagtt ctggatagcg gtaaacaacg
    15241 gcgcatcctg acggaactct ggcgtacggc ccatgactgc cgccagatca aagcggaaac
    15301 catcgacgtg gcaggtttct acccaataac gcaggcaggc gctggcataa tccaccaccg
    15361 ccggatgact caaattgagc gtgttgccgc aaccggtcca gttgtgataa tcgccgtctt
    15421 ctcttatcca ataatagcta cggttatcga tcccacgcag cgagaataac gggccgtcga
    15481 ggtccagttc cgcactatgg ttgagcacga tatcaagaat gacttcgata cccgctttat
    15541 gcagtgcttt gattgcatcg cgaaactcat ccagcgccgt ttctggcgag caggcatacg
    15601 ccggatgcag cgcaaacatc gccaccgggt tgtaacccca gtagttactt agccccatgc
    15661 gttgcagacg tagttcactg gcaaactgcg ccactggcag cagttccagc gcggtaatgc
    15721 ccaattgttt caaatagttg atcatcaccg gatgcccgag ggctttataa gtgccacgga
    15781 tctcgaccgg gatctccggg tgcaagtacg ttaatccttt gacatgggct tcataaatga
    15841 tggtgctgcc ccacggcgtg cgcggcgggg catcatcttc ccagtcatag tgatcaacca
    15901 ccactacgca tttcggcgca atggcggcgt tgtcgcgata gtcaggttca ttatgaccgg
    15961 cgtgcagcag cgggttatct ttaaactccc cgtcaatttg ccgcgcgcaa ggatcaatca
    16021 acaacttcgc cgggttaaag cgatgcccct cggcgggttg ccaggggcca tgaacgcgat
    16081 aaccataacg caaacccggg cgcgcatccg gcagataacc gtgccaaatg tcgccactgt
    16141 gccctggcaa gtcatagcga tgttcctggc cattggcgtc aaagacacac agttctaccc
    16201 gctcggcatg agcggagaaa agtgtgaagt tgacgccctg accgtcgtaa tgcgcgccga
    16261 ggggagcggg tttgccaatg gcgagttgtg tcattctgcc tcccgaacca gccagatagt
    16321 ggccagcggt ggtagcgtca ggcttagtga atgctgacga ccgtggctgg caatctcatc
    16381 gctgtgtacc gtgccgccat tgcctgcatt actgccgtga tagtgcatgg aatcggtatt
    16441 gaggatttca cgccatttgc ccggctggtt tatgccgaag cgataatcat gacgcggtac
    16501 cggcgtaaag ttactggcaa cgatgatttc gttaccctct ttatcgcgac gcacaaagat
    16561 cagcaccgag cgttctttgt catccaccac cagccattca aagccgtacg ggtcaaaatc
    16621 cagttcatgc attgctttat ggtggcggta ggtgaggttc agatcgcgca ccagacgctg
    16681 gacaccgtgg tgccagttat cgccgccttc caacagatgc cagtcgaggc tggcgtcatg
    16741 gttccactcg cggccctggg caaattcgtt ccccatgaac agcagtttct tgcccgggaa
    16801 tgcccacatc cagccatagt aggcgcgcag gttagcgaat ttctgccatg cgtcgcccgg
    16861 catgcggtcg agaatcgatt ttttaccgtg gaccacttca tcatgcgaca acggcaggac
    16921 gaagttttca gtgtagttgt agagcatccc gaaggtcagt ttatcgtgat gatactgacg
    16981 ataaaccggg tcgagcttca tgtagtccag ggtgtcatgc atccagccga ggttccactt
    17041 gtaccagaag cccagaccgc ccatatcctg cggacgagaa acgccaggga aatcggtaga
    17101 ctcctcagcc attgtcaccg caccggaaac ctgctcacca agaatacggt tggtattacg
    17161 caagaattca atcgcttcaa gattctcgcg cccgccaaat tcgttcggga tccactcccc
    17221 ctctttacgg ctgtagtcgc gataaatcat tgacgccacc gcatcgacgc gcagcgcatc
    17281 aataccaaaa cgttcaatcc agtaaagcgc gttaccgaca aggaagttac tgacttcacg
    17341 gcgaccatag ttgtagatca gcgtgttcca gtcctgatga tagccttcac gcggatcgct
    17401 gtgttcatac aagttcgtgc catcaaattc ggcaagcgca aagtcatcgg tcgggaagtg
    17461 gcctggtacc cagtcgagaa tcacgttcag accagctgcg tgtgcggcat caatgaaata
    17521 acggaagtcg tcgcgagtac caaaacggcg ggttggtgca tacaggccgg ttggctgata
    17581 accccaactg ccatcgaagg gatgttcgtt aatgggcagt agttcgaggt gggtaaaacc
    17641 catccattta gcataaggca ccagttgatc ggccagctcg cggtagctca accagaaatt
    17701 gttgtcggtg tggcgacgcc aggagcccag gtgaacttca taaatagaga ttggcgcatc
    17761 aaactgattc gcttttttgc actcttcagt ctgcacaacc ttttccggta gcccgcaaat
    17821 aagagacgcg gtttccgggc gcatttgcgc ttcgaaggca taagggtcgg acttcagacg
    17881 caagttgcca ttggcatcaa tcatctcgta tttatagagc tgaccgttat gcgccccagg
    17941 gataaacagt tcccagatgc cgctctcttt acgcaggcgc atcgggtgac ggcgaccgtc
    18001 ccagtagttg aattgcccaa ccaccgagat ccgacgggcg tttggagccc agacagagaa
    18061 acgcgtacct gtgacgccat ccatagtatc tgcatgcgcg cctaaggttt catacgggcg
    18121 caggtgagta ccttcagata atagccaggc atccatttcc tggattagcg gaccaaaacg
    18181 gtaaggatca tcaatcaggt tttgctgacc atgccagaca acagccaact gatagcggaa
    18241 aaaattctta cgtcgcggaa tgacgccgct aaagaatccc cgtgagtcga gacactccag
    18301 ttttgcgagt ttgcgcccgg ttttcggttc aatcacccac acatcggtag cgtcgggtaa
    18361 aagggcacgg acttccagtc ccgcggtggt tttatgcatt cccagtacgg aaaaaggatc
    18421 cgcaaaatgg cctgcaatta gcgcgttaat cacgtctcta tcgatacgat cggacatgct
    18481 tgtcttcctg ttttattgtg tcaccccatc caattggatt tttgacttct ggttgtgaca
    18541 tttttttgac ctgaacggcg cagcactctg tgcatcctct ctgcgtcgtc ctcacttcag
    18601 gtaaggctgt gaatactcat gtattcagcc acccttaaag aatagccaat gctctattta
    18661 actcccggta aatcatgaaa catctgcgct tactcctgta ttacgcacta acaggggcgg
    18721 catcgcgccc cagatttaat gaataaagat tacgccagtt gacgaagcat ccgacgcagc
    18781 ggctccgcgg ccccccacag cagctggtcg cccacggtaa aggctgacag gaactctggt
    18841 cccatattca gcttacgcag gcggcctacc ggcgtggtca gcgtgccggt aacggcagct
    18901 ggggttagct cacgcatagt gatttcccga tcgttcggaa cgactttcgc ccacggattg
    18961 tgcgcagcca gcagttcttc gacggtcgga atagacacat cttttttcaa tttaatagtg
    19021 aatgcctggc tgtggcagcg caatgccccg acacgcacac ataaaccatc tacctgaatt
    19081 acggaagatg tgttgaggat cttgttggtt tccgcctgcc ctttccactc ttcgcggctc
    19141 tgaccgttat cgagctgttt gtcgatccac ggaatcaggc tacccgccag cggcacgcca
    19201 aagttatcca ccggcagctc accgctacgg gttaaggttg tgactttgcg ttcgatatcg
    19261 agaatagcag aggacggggt cgcgagttca tctgccacat ggccatacag atggcccatc
    19321 tgggttaata actcacgcat atgtcgcgca ccaccgccgg aagcggcctg gtaggttgca
    19381 acggacaccc aatcaacaag atcattggcg aataaaccac ccaacgacat caacatcagg
    19441 cttacggtac agttaccgcc aacaaaagtc ctgatgccat tatttaatcc gtcggtaatg
    19501 acgtcctgat tgacggggtc aagaatgatg atggcgtcat ctttcatgcg cagagacgat
    19561 gctgcgtcaa tccagtaacc ttgccatccg ctttcacgaa gctttggata gatttcgttg
    19621 gtataatcgc cgccctgaca ggtcacaatg atatcgaggg cctttagcgc ctccagatca
    19681 aaggcatcct gaagtgtgcc agtggttccg ccaaaagacg gcgctgcctg gccaagctga
    19741 gaagtagaaa agaagacagg gcgaatggcg tcgaagtcgc gctcttcaac catgcgttgc
    19801 atgagaacgg agccgaccat accgcgccag ccgataaaac caacattttt cataagcgtt
    19861 ttttcctgca aagatgtgtg ctgtataaat gtgccggtct cctcttggca catctttcac
    19921 catacaaaaa gcagccaaag tcgcaagtga aattaatcaa tgatagcgaa gccatcagta
    19981 atgcgactta tcctgctttg ttagcacgca gaaagtccgc ggcaattatc agggaatttg
    20041 agttatgaat gaaatcattt ctgcagcagt tttattgatc ctgattatgg atccgctcgg
    20101 aaacctacct attttcatgt ccgtactgaa acatactgaa ccgaaaagac ggcgggcaat
    20161 catggtgcga gagttgctta ttgctctcct ggtgatgctg gtgttcctgt ttgcgggtga
    20221 gaaaattctg gcatttctca gcctgcgagc agaaaccgtc tccatttctg gcggcatcat
    20281 tctgtttcta attaccatta agatgatttt ccccagcgct tcaggaaata gcagcgggct
    20341 tccggcaggt gaagagccat ttatcgtgcc gttggcaatt ccgttagtcg ccgggccgac
    20401 tattctcgcc acgctgatgt tgctgtctca ccagtaccca aatcagatgg gacatctggt
    20461 gattgctctg ctgctggcct ggggcggcac ctttgtcatc ctgctacagt cttcgctatt
    20521 tttacgtctg ctgggcgaga aaggggtgaa cgcacttgaa cgcctgatgg gattgattct
    20581 ggtgatgatg gcaacccaga tgttcctcga cggcattcga atgtggatga aggggtaacg
    20641 aggagagttt ttcgaaaaga acccggtagc gtcacaacta ccgggcgaac aaacttaact
    20701 caacagctgg aacgcaatca tcccaacgat ggcaccaaca gtgccgagga tggtttccat
    20761 catggtccag gttttcagcg tttcggcttc ggtcgcgccg gtaaatttac cgaacaacca
    20821 gaaaccggcg tcgttaacgt ggctgacaac aatcgaacca ccagcgatac aaatcgacag
    20881 tgccgccatt tgcgcaccgg agtagttcag ttgttcaatg accggcatca ccagccccac
    20941 cgccgttaaa caggcaacgg tggcagaccc ctgaatgatg cgcactgcag cggccagcac
    21001 gaagcaggtg atagcaatcg gcaggcccat gccggttaac gcttcgccca gtgccggacc
    21061 tacgccagag tcaaccagca cctgtttgaa cacgccgcct gcaccaatca ccagcagaat
    21121 gatccccgcc ggttgtagcg cgtgaccgca aatctccatc actttgtctt ttggcatgcc
    21181 ctgacgcatt gccaggccat aaatcgctac cagacaagca accagaatcg cggtaaatgg
    21241 atgaccaata aactcgaacc attcgtaagc agttgatcct tctggcacaa aacgagcggc
    21301 aatggttttc agccccacca gcaccagcgg caacaggatc agcgacaggc tgaatccgaa
    21361 ggacggcatt ttgccttcgc cgagatgcgg ttcgctgatg tcgtcaggaa tatgcaactc
    21421 cacgtaacgg ctgatgaaat taccccacag cggcccggca ataatcattc ccggaattgc
    21481 cgcacacaga ccaatcagga tcatccagcc aaagtcggca ttcatctgcg atgccagcag
    21541 catcggcgct ggtccaggca ccaaaaatgc cgccgctgcc gccacgcctg cgaataatgg
    21601 gattaccagc ttcaccaggt tcgtaccggt gtggcgcgcc attgagaaag caacgctaat
    21661 cagcagaact atcgccactt caaagaacag cggtagcgca cagaccaacc ccgcaaggcc
    21721 gatggcataa tgcgcgcggc tgtgaccgaa ggatttgagc attttgacgg caatctgatc
    21781 gactgcgccg gtttcatgta agatcttgcc aaacatagct cccagggcga caaccaccgc
    21841 caggaagccg agggtgcctc ccatcccttt ttccatcgtc gctgcgattt tatcgagcgg
    21901 cataccggaa aaaaggccag cccccatgga caccaccatc aaagccagga aagcgtgcat
    21961 acgcgccttc atgactaaaa acagcagcag taaaacagac cctactgctg ttaaaacaag
    22021 cgttaatgta gtcactactt atttgccttt tttaataacc tcaatggtgc ttgccacaac
    22081 accttccagc ggttgatcga tatccaccac cagtacatcg gtttcgtccg cacccggctc
    22141 ctgcagcgtt tcaaactgcg tcaccaacat ttgggtttta aagaaatggc ctttgcgcgc
    22201 tttcaagcga ctttcaatca catcaaaatc gcctttcagg tagatgaaag agagattcgg
    22261 attaccttca cgcagcaagt cgcgatagtg ctttttcagt gcagaacaga cgatcagcga
    22321 cactttattg gtgcgctgca tagcaaacgc ggcgtcgttc agcgcctgca gccacggttt
    22381 gcgatcgtca tcattcagcg gttcgccaga cgccattttt tcgatattgc gccgtggatg
    22441 gaggaaatcg ccatcaagaa acgcggcatg aagttgatgc gccacttcac tggcgaccgc
    22501 agatttgccg ctgcccgata cgcccatcaa gacgtaaatg tggtgatcat ggttagtcgt
    22561 gctcaaagtg gtgctcccac aatacaagaa ttaaaaatgt tacgggtaac tgttatcggt
    22621 aacattgtcc agccggacaa tatcagaagc aatatccatc cgtgccttaa gtgtataagt
    22681 gtgagctact tcaaatttgt aggcttaaat agatccgccc ggtgacaagg tgaaacctaa
    22741 atctaacatt ttcggtgtca cagattcgcc acgaatacgc gccagcaggc gttcagcgcc
    22801 aatactgccc atccgctcac gcggcgtcag cacgctcgct aaccgcggct ccatcacctg
    22861 gccaatgtca tgaccgtgga aaccggcaat cgccatatcg tcaggaacct tcaatcccag
    22921 acgctgacat tcaaacgccg cgccgaccgc caggtcatcg ttggtacaga acacgccatc
    22981 cagctgcgga tattcccgcc gcgcctggcg aatcagttca ataccggaag agtaagaaga
    23041 agattgctca accatcacgc tatacggcac cagacctgca tccagcatcg cctgttcgta
    23101 tcccttctgt ttgatgatag tacgttcgtc gagacgcgcg ccgagatagg caatgtggca
    23161 atgcccgcga gcaataatgg cggtggtcat ctggcgtgcc gcttcaaagt tatcaaaacc
    23221 gacggcgata tcgaggcacg gcgactggct gtccatgagt tccaccaccg ggatccccgc
    23281 cacttcaatc atctttaagg tgcgcggcgt gtgggtacgt tcggtgagga tcagtccgtc
    23341 gatattccat gagagcatgg attcgagacg ttcttgctcc atttccggtt tataaccgta
    23401 gtgagccagc atggtctgat aaccgtgcgc gtcggtgacg ctttcgattc cgcgtaatac
    23461 ttccgcgaaa acctggttgg tgagagaagg taacaggacg ccaatcgccc ggctggtagc
    23521 gttagagagg atatcgggcg cacgattggg aatatagccc agttcatcaa gagcggcggc
    23581 aatcttgccg cgtagagcga cggaaacctg ctccgggttg cgtaaaaaac ggctgaccgt
    23641 cattttggtc acgcctacac ggtcagccac atcctgaagt acgggtcttt tctttttcat
    23701 cgtcctgaag gtacaaaaga gatagattct cttagtttaa cacggacgca tcagaacctt
    23761 cccggggaaa acagtgcttg tttacaatta ttagatatac actaaatcaa ttgcatcgca
    23821 ttgtgctaac gacgcggcaa aaccccggaa gcgtgcatca gcacgcgacc ggagcgagcg
    23881 aggaagcctg caaaaaggca ggcttcaaaa atgccgtgga gctttaaacc ggcggcagat
    23941 cgaacagtaa cacttcgctg tcgctatccg catggatgga aattgcctgc tcatcccaga
    24001 ttgccagacc atcgctggtc gaggctttca cgccgttaat ggtgacattg cctttcacca
    24061 cctggatcca gacgcggcgt tcagcggcaa tctgatgcac cgactgctca tctttcagca
    24121 acgcccagcg gtacagttcc atatcctgat gcactttcaa tgaaccatct cgagcatccg
    24181 gcgagagcac cagctgtttg ccctgcacgg catcgaagcg acgctgttca taacgcggcg
    24241 taataccgtt ttcttcaggc atgatccaga tctgatacag atgcagacgc tcggtgctgc
    24301 ttgggttgta ctctgagtga cgaatacccg tacccgcact cataatctgg aactcacccg
    24361 ccggaacctg ctctttattg cccatgctgt cctgatgctc aacagtacct tccagcacgt
    24421 aggtcaaaat ttccatatct ttatgcgggt gagtgccgaa gccctgccct gcttcaatca
    24481 cgtcgtcgtt aatcacgcgc agcgcggaga agcccataaa gttcggatcg taatagttgg
    24541 caaaagagaa agtatgccag gagtccagcc agccatgatt tgcatgacca cgttcatttg
    24601 ctttgcgtaa gtagatcatt tgtattcgcc ccctgaatga tttcgatggg cttagtgtgg
    24661 acccgatctg cctgggatga tagagggtga aaattgaccc ctctgttcaa aaaaattgaa
    24721 ctattcgagg ggccatcaaa cttacttagc gagagttact gtggagggag aggcttgctc
    24781 aaatccgcgt tcaaggattt ccagattggt aagaacttca gattccttga cgtaatttgg
    24841 cgcaccgtgg gtgatggttt gatacaacgc atcataaacg cgcccgtaat cgcccatctc
    24901 cggcttcatc tcttctctga ccgtcacgcc ctcgtcattg acatactcca gcacaccgac
    24961 cgaatcatcc gctgcgaatc ccggttcgcc cggcataata ttagccttca ggctggtttc
    25021 ctgctggtcg ataccgtatt taataaacga acctttctta ccgtgaacga taaatttcgg
    25081 ataatcgatt ttcaccagat ggctggtttt gacgatggct ttcaggtcgc cataaaacag
    25141 ttgcgcttca aaggtgtcgt caggattggc tttattacgc aggctgcgga tgtcataagc
    25201 gacgtgatcc gggcgaccga acagagaaat aatctggtcc atcgtatgca caccaaggcc
    25261 atagaacgcg ccatcctgcg gcagtccagg tttggtttct gccaccgggc ggtaatagtc
    25321 aaaatggctt tccacttcga caatctcttc cagcttgccg ctttcaatcg cttttttcgc
    25381 cgtcaggaag caggaatcaa agcgacgatt ctgatatggc gtgacggtca gccctttgct
    25441 tttcgccagt gcaaacagct ctttcgcctg cgcaagtgtc ggagtgaacg gtttttcgac
    25501 cagcacattt ttcccggctt ccagcgcgcg cttcgcgtac tcgaagtggc tgtccgcatg
    25561 ggtgcagaca acaaccaact taacatcggg atcgtttagc acttcgtcga gatcgctggt
    25621 gaaatggata tgagagtaaa tgggagcctg ttcttccggc ttcgcatggc gacgaaaaat
    25681 atgcgcgaca tgccagctat ccttgcggtt aagtacatac ggcagatggt aacgggtggt
    25741 gcttttgccg aagccaataa aggcgcagtt gatgaccatg atttcgtcct ttttaaggtg
    25801 gttatttaca ccttagcgca aagcggacgt ggttcctact ggagtgcgca taacgaacac
    25861 aagcactccc gtggataaat tgagaacgaa agatcaaaaa aaaagccagc acccggctgg
    25921 ctaagtaata ctggaagcaa tgtgagcaat gtcgtgcttt caggttctcc gcgagggtct
    25981 tcctgatcgc gaggcaataa taatcattct cattcgcact tgtccaacac tttttgcaaa
    26041 aaaatgcatt taactcgcat ttgaaagtca atgatgttga aagggacatt taccccaaag
    26101 aggacaaagg aatgagtgag atagtaatac gccacgcaga aacacgggat tacgaggcca
    26161 tcaggcagat tcacgcccag ccggaggtgt attgcaacac actacaggtg cctcatcctt
    26221 ccgatcatat gtggcaggag cgactcgccg atcgtcccgg catcaagcaa ctcgtcgcct
    26281 gtattgatgg agacgtcgtg ggccatctca ccattgacgt gcaacagcgc ccacgccgca
    26341 gtcatgttgc cgattttggt atctgtgtcg actctcgttg gaagaaccgc ggcgtcgcca
    26401 gcgccctgat gcgagagatg attgaaatgt gcgacaactg gttgcgggta gatcgcattg
    26461 aactaaccgt gtttgtcgat aacgccccgg caattaaggt ctataaaaaa tacggctttg
    26521 aaattgaaga gactggtaag aagtacgcat tgcgtaatgg tgaatatgtc gatgcatatt
    26581 atatggcgcg ggtgaagtaa gatagtgccc tttttctgag atggaaaaag ggtgtcattc
    26641 aaaatcgaca taccttcctt taaggtattt atttacccaa tacatatatt gagatttaac
    26701 tcataattac ttcctaaagt gtaatatttt attttttaat atatacgcct acaatttcct
    26761 ggagtaaata aataacaatt aacaagcata atattgccat tgataaaata gcatgccata
    26821 aaaggacttt tcagggatga gtaatattgt ttacctgaca gtaacgggag aacaacaagg
    26881 aagcatctcc gcaggttgtg ggacttctga gtctacaggt aatcgttggc agagcgggca
    26941 tgaggatgaa atatttacat tctcactctt aaataatatt aataatacgg ggcttggttc
    27001 acagttccat ggtataacat tttgtaaatt aattgataaa agcactccat tatttattaa
    27061 ttccattaac aataatgaac aattatttat gggatttga
//
LOCUS       DAZKHC020000061        26247 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZKHC020000061 DAZKHC020000000
VERSION     DAZKHC020000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.061"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(102..182)
                     /gene="shoB"
                     /locus_tag="ACHOSM_RS14725"
                     /old_locus_tag="pgaptmp_002945"
     CDS             complement(102..182)
                     /gene="shoB"
                     /locus_tag="ACHOSM_RS14725"
                     /old_locus_tag="pgaptmp_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HGU4559428.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(603..983)
                     /gene="acpS"
                     /locus_tag="ACHOSM_RS14730"
                     /old_locus_tag="pgaptmp_002946"
     CDS             complement(603..983)
                     /gene="acpS"
                     /locus_tag="ACHOSM_RS14730"
                     /old_locus_tag="pgaptmp_002946"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGU4559429.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(983..1714)
                     /gene="pdxJ"
                     /locus_tag="ACHOSM_RS14735"
                     /old_locus_tag="pgaptmp_002947"
     CDS             complement(983..1714)
                     /gene="pdxJ"
                     /locus_tag="ACHOSM_RS14735"
                     /old_locus_tag="pgaptmp_002947"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HGU4559430.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQVSLFIDANEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1726..2454)
                     /gene="recO"
                     /locus_tag="ACHOSM_RS14740"
                     /old_locus_tag="pgaptmp_002948"
     CDS             complement(1726..2454)
                     /gene="recO"
                     /locus_tag="ACHOSM_RS14740"
                     /old_locus_tag="pgaptmp_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGU4559431.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGELVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2466..3371)
                     /gene="era"
                     /locus_tag="ACHOSM_RS14745"
                     /old_locus_tag="pgaptmp_002949"
     CDS             complement(2466..3371)
                     /gene="era"
                     /locus_tag="ACHOSM_RS14745"
                     /old_locus_tag="pgaptmp_002949"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGU4559432.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINALILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3368..4048)
                     /gene="rnc"
                     /locus_tag="ACHOSM_RS14750"
                     /old_locus_tag="pgaptmp_002950"
     CDS             complement(3368..4048)
                     /gene="rnc"
                     /locus_tag="ACHOSM_RS14750"
                     /old_locus_tag="pgaptmp_002950"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGU4559433.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4321..5295)
                     /gene="lepB"
                     /locus_tag="ACHOSM_RS14755"
                     /old_locus_tag="pgaptmp_002951"
     CDS             complement(4321..5295)
                     /gene="lepB"
                     /locus_tag="ACHOSM_RS14755"
                     /old_locus_tag="pgaptmp_002951"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGU4559434.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5311..7110)
                     /gene="lepA"
                     /locus_tag="ACHOSM_RS14760"
                     /old_locus_tag="pgaptmp_002952"
     CDS             complement(5311..7110)
                     /gene="lepA"
                     /locus_tag="ACHOSM_RS14760"
                     /old_locus_tag="pgaptmp_002952"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGU4559435.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7308..7787)
                     /gene="rseC"
                     /locus_tag="ACHOSM_RS14765"
                     /old_locus_tag="pgaptmp_002953"
     CDS             complement(7308..7787)
                     /gene="rseC"
                     /locus_tag="ACHOSM_RS14765"
                     /old_locus_tag="pgaptmp_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HGU4559436.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVVLPPGLVRFETSSEDASQ"
     gene            complement(7784..8740)
                     /gene="rseB"
                     /locus_tag="ACHOSM_RS14770"
                     /old_locus_tag="pgaptmp_002954"
     CDS             complement(7784..8740)
                     /gene="rseB"
                     /locus_tag="ACHOSM_RS14770"
                     /old_locus_tag="pgaptmp_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HGU4559437.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8740..9390)
                     /gene="rseA"
                     /locus_tag="ACHOSM_RS14775"
                     /old_locus_tag="pgaptmp_002955"
     CDS             complement(8740..9390)
                     /gene="rseA"
                     /locus_tag="ACHOSM_RS14775"
                     /old_locus_tag="pgaptmp_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HGU4559438.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9423..9998)
                     /gene="rpoE"
                     /locus_tag="ACHOSM_RS14780"
                     /old_locus_tag="pgaptmp_002956"
     CDS             complement(9423..9998)
                     /gene="rpoE"
                     /locus_tag="ACHOSM_RS14780"
                     /old_locus_tag="pgaptmp_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HGU4559439.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9995..10150)
                     /gene="rseD"
                     /locus_tag="ACHOSM_RS14785"
                     /old_locus_tag="pgaptmp_002957"
     CDS             complement(9995..10150)
                     /gene="rseD"
                     /locus_tag="ACHOSM_RS14785"
                     /old_locus_tag="pgaptmp_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HGU4559440.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10406..12028
                     /gene="nadB"
                     /locus_tag="ACHOSM_RS14790"
                     /old_locus_tag="pgaptmp_002958"
     CDS             10406..12028
                     /gene="nadB"
                     /locus_tag="ACHOSM_RS14790"
                     /old_locus_tag="pgaptmp_002958"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HGU4559441.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(12013..12750)
                     /gene="trmN"
                     /locus_tag="ACHOSM_RS14795"
                     /old_locus_tag="pgaptmp_002959"
     CDS             complement(12013..12750)
                     /gene="trmN"
                     /locus_tag="ACHOSM_RS14795"
                     /old_locus_tag="pgaptmp_002959"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HGU4559442.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12882..14216
                     /gene="srmB"
                     /locus_tag="ACHOSM_RS14800"
                     /old_locus_tag="pgaptmp_002960"
     CDS             12882..14216
                     /gene="srmB"
                     /locus_tag="ACHOSM_RS14800"
                     /old_locus_tag="pgaptmp_002960"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HGU4559443.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14425..15306)
                     /gene="yfiE"
                     /locus_tag="ACHOSM_RS14805"
                     /old_locus_tag="pgaptmp_002961"
     CDS             complement(14425..15306)
                     /gene="yfiE"
                     /locus_tag="ACHOSM_RS14805"
                     /old_locus_tag="pgaptmp_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4559444.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            15409..15996
                     /gene="eamB"
                     /locus_tag="ACHOSM_RS14810"
                     /old_locus_tag="pgaptmp_002962"
     CDS             15409..15996
                     /gene="eamB"
                     /locus_tag="ACHOSM_RS14810"
                     /old_locus_tag="pgaptmp_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HGU4559445.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(16052..16435)
                     /gene="grcA"
                     /locus_tag="ACHOSM_RS14815"
                     /old_locus_tag="pgaptmp_002963"
     CDS             complement(16052..16435)
                     /gene="grcA"
                     /locus_tag="ACHOSM_RS14815"
                     /old_locus_tag="pgaptmp_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HGU4559446.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16738..17427
                     /gene="ung"
                     /locus_tag="ACHOSM_RS14820"
                     /old_locus_tag="pgaptmp_002964"
     CDS             16738..17427
                     /gene="ung"
                     /locus_tag="ACHOSM_RS14820"
                     /old_locus_tag="pgaptmp_002964"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGU4559447.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQHGETPI
                     DWMPVLPAESE"
     gene            complement(17475..18512)
                     /gene="yfiF"
                     /locus_tag="ACHOSM_RS14825"
                     /old_locus_tag="pgaptmp_002965"
     CDS             complement(17475..18512)
                     /gene="yfiF"
                     /locus_tag="ACHOSM_RS14825"
                     /old_locus_tag="pgaptmp_002965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU4559448.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNLRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18719..19138
                     /gene="trxC"
                     /locus_tag="ACHOSM_RS14830"
                     /old_locus_tag="pgaptmp_002966"
     CDS             18719..19138
                     /gene="trxC"
                     /locus_tag="ACHOSM_RS14830"
                     /old_locus_tag="pgaptmp_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HGU4559449.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            19207..19905
                     /gene="tapT"
                     /locus_tag="ACHOSM_RS14835"
                     /old_locus_tag="pgaptmp_002967"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             19207..19905
                     /gene="tapT"
                     /locus_tag="ACHOSM_RS14835"
                     /old_locus_tag="pgaptmp_002967"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HGU4559450.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19937..22597
                     /gene="pat"
                     /locus_tag="ACHOSM_RS14840"
                     /old_locus_tag="pgaptmp_002968"
     CDS             19937..22597
                     /gene="pat"
                     /locus_tag="ACHOSM_RS14840"
                     /old_locus_tag="pgaptmp_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HGU4559451.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPLALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQTVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22711..24066
                     /gene="pssA"
                     /locus_tag="ACHOSM_RS14845"
                     /old_locus_tag="pgaptmp_002969"
     CDS             22711..24066
                     /gene="pssA"
                     /locus_tag="ACHOSM_RS14845"
                     /old_locus_tag="pgaptmp_002969"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HGU4559452.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYDAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            24112..24435
                     /gene="yfiM"
                     /locus_tag="ACHOSM_RS14850"
                     /old_locus_tag="pgaptmp_002970"
     CDS             24112..24435
                     /gene="yfiM"
                     /locus_tag="ACHOSM_RS14850"
                     /old_locus_tag="pgaptmp_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HGU4559453.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(24432..25730)
                     /gene="kgtP"
                     /locus_tag="ACHOSM_RS14855"
                     /old_locus_tag="pgaptmp_002971"
     CDS             complement(24432..25730)
                     /gene="kgtP"
                     /locus_tag="ACHOSM_RS14855"
                     /old_locus_tag="pgaptmp_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HGU4559454.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEERKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILQAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGIRL"
     gene            complement(26056..26171)
                     /gene="rrf"
                     /locus_tag="ACHOSM_RS14860"
                     /old_locus_tag="pgaptmp_002972"
     rRNA            complement(26056..26171)
                     /gene="rrf"
                     /locus_tag="ACHOSM_RS14860"
                     /old_locus_tag="pgaptmp_002972"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6331 a         6924 c         6551 g         6441 t
ORIGIN      
        1 actaagttaa tttatatagt atctgccaag acacttattt atagttatta aaggcgcgtc
       61 cgattggttc accggacgca ccttaagtac gtttccttgt gttataagaa cagaaggatc
      121 agctgtaaaa cagcaatgat gattttgatg acccgtttaa tcaggtatcg gcaatcagtc
      181 attcgttttt ccttaaacaa ggaatgcagc cattcagatt agcccttaca tctccccaaa
      241 actgaacgtg agagttattg agggtgcatg ctgcactcca caccagagct ttgacgacac
      301 cactcgtttc aatgggggaa ttctgtggca tggtgtaaag cacagcaaaa tcttcaataa
      361 cgaagccaat tttaatgtac ttacgaattg gcggtcacgt taatctttcc atcaatattg
      421 ctttcttcgt aaaggctcga gtttttatgc taaagattgc aagttgcttg taaaagataa
      481 gtacactgat ccataatcgc tgttgttgag ggtgcatgct gcacaaaatt aaagttaaaa
      541 aataaaaccc ccgttcctta ccagttcggg ggttttactt tttaaagaga acggtattat
      601 ttttaacttt caataattac cgtggcacaa gcatagtgcc gctcatctgc cagcgttaca
      661 tgcatattta caacgccgag cttttccgcc agttttaatg cctcacccca tagccgtagc
      721 cgtggtttgc cgagctcatc attgaatact tcaaattgat taaacgccag accattgcgg
      781 atcccggtgc caaacgcttt tgctgcggct tctttcacag caaaacgctt cgccagaaaa
      841 cgcaccggct ggtggtgcgt tttccagatt gcccattcgt tatcgcttaa tacgcggcgt
      901 gccaggcgct caccggatcg ggcgatcact gcttctatgc gagcgatctc tacgatatcc
      961 gtgcctaaac ctaatattgc cattagccac gcgcttccag catcagacgc ttcatttctg
     1021 ccaccgcatc tttcagtccg gtcatcactg cacggccaat aatggcatga ccgatattca
     1081 gttcatgcat ctcagggatg gcggcaatgg ctttcacgtt gtgataggtc agaccgtgtc
     1141 cggcgttaac tttcagaccg agactagcgg caaaggtcgc ggctttggcg atacgcacca
     1201 gctcttgcgc ctgttcggcg tcagttttgg catcagcata gcaaccggtg tggatctcga
     1261 taaacggtgc gccaacctct gccgcagctt tgatctgctc ttcattggcg tcgataaaga
     1321 gagaaacctg aatcccggca tctgtcagac gtttgcaggc atcgcgcatt ttttcacgct
     1381 gccctgcaac gtccaggccg ccttcggttg ttacttcctg acgcttttcc ggtaccaggc
     1441 agcaaaaatg tggcttcgtc tcaacggcga tcgccagcat ctcttcggtc actgccatct
     1501 ccagattcat tcgggtatcc agcgtctgcc gcaggatgcg cacatcgcgg tcagtaatgt
     1561 ggcggcgatc ttcacgtaaa tgcacggtaa tgccgtccgc tcccgcctgc tcggcaataa
     1621 acgccgcctg caccggatcc gggtaagcag taccgcgtgc gttacgcagc gttgcaatat
     1681 ggtcaatgtt gacgcctaac agtaattcag ccatgacaat cctcatcatt cataatgtgt
     1741 tttcaccgtt cgcttaggca taaactgccg gaacagttcc ctgctcttta atggtttacc
     1801 gccaagatac ggcttaagcg ccatgcgggt aaagcgtttc gcggcgcgta gtgtgtctgc
     1861 gtcaggaaat tcccgcgcgt ttaacgcttt taactgcctt ccggtgaacg ttttattgtc
     1921 gataacgacg cttgcgataa accctttttc ttcgcgataa cgatacgtca tggtgtcatc
     1981 taccagctcg ccgctacccg cgcaatgggt aaaattgacg ccatagccca gatgcccgag
     2041 cagtgccagt tcaaagcggc gcagcgcggg ttctggcgta ccagtggcac ctgcaagaga
     2101 ctgaatgcag tgcaagtaat cgaaaaagag ttcagagaag cgcgtctcgt attccagtac
     2161 gcgggagaga agttcgttga tgtacagacc gctgtaaagc gtgataccgc ttaatggcag
     2221 cgccagcgag acagcttcag cactgcgcag cgttttgact tcgccacgcc cgccgaaacg
     2281 tagcaagaga ggggtgaaag gctgtaatgc acctttcaga gtagagcgtt tagagcgtgc
     2341 gcctttggca accagacgca cgcgacccga ttcctccgtg aagacgtcca gcatcaggct
     2401 ggtttcgctc cacgggcgac tatgcaggac aaatgcgcgc tgccagcctt ccatcggagt
     2461 gactcttaaa gatcgtcaac gtaaccgaga ctgcgcagtg cgcgttcgtc gtcggcccaa
     2521 ccggatttca cttttaccca cagttcaagg tggacaggcg cttcgaacat ttcctgcatg
     2581 tctttacgcg cttcaatccc gatggttttg atcttggccc ctttgttgcc aataaccatc
     2641 ttcttctgcc cttcacgctc aacgagaatc aaagcattga tgtcataacc accgcgttcg
     2701 ttagagacga aacgttcgat ctccacggtc acggagtacg gcagttcagc gccgaggaaa
     2761 cgcatcagtt tttcgcggat gatttcagac gccataaaac gctgtgagcg atcggtgatg
     2821 taatcttccg ggaagtgatg ggtcgcttca ggcagatgct tacgcacgat tgctgcgata
     2881 gtgtcgacat tcagcccggt ttcagcagag atcggcacga tatcgaggaa gttcatctga
     2941 cttgccagga actgtaagtg cggcagcaga tcggcttttt cctgcacgtt atccactttg
     3001 ttcaccgcga ggatcaccgg cgctttgcca tcgcgcagtt tgttgagcac catttcgtcg
     3061 tccggcgtcc agcgggtgcc ttcaacgaca aaaatcacca gctcaacgtc gccaatagag
     3121 ctgctcgccg ctttgttcat caggcggtta atagcgcgtt tttcttccat atgcaggcca
     3181 ggggtatcaa cgtagatcgc ctgatacgcg ccttcagtat ggatccccac aatgcggtga
     3241 cgagttgttt gcgctttgcg ggaggtgatg gagatcttct gccccagcag tttgttcaac
     3301 aatgtggatt tgccaacgtt cggacgtccg acgatggcaa taaatccgca gtaactttta
     3361 tcgatgctca ttccagctcc agttttttca acgcctgttc ggcggcagcc tgctcagcct
     3421 tacgacggct tgaacctgtg ccaaccaccg gttcactcag gccgctgacc tggcagtgga
     3481 tagtaaattc ctgatcgtgc gcttcgccac gtacctggac taccagataa gtcggcagcg
     3541 gcagatggcg accctgcaaa tattcttgca agcgcgtttt cggatctttt tgtttatcgc
     3601 ctgggctaat ttcgtccaaa cgagtttgat accagttgag gattaatttc tcgacggttt
     3661 gaatatcact gtcgaggaat acgccaccaa ttaatgcttc gacggtatcg gcgagaattg
     3721 actcacgacg aaatccaccg cttttaagtt cacctggtcc taaacgtaag cactcgccta
     3781 actcaaattc gcgcgccagt tccgccagcg tattgccacg gaccagcgtg gcgcgcatcc
     3841 ggctcatatc gccttcatcc acacgaggga aacggtgata aagcgcattg gcgataacgt
     3901 agctcagaat agagtcgcct aaaaattcta aacgctcgtt atgtttgctg ctggcactac
     3961 gatgagttaa tgcctgctgc aacagttcct gatgattaaa agtgtagccc agcttccgtt
     4021 gaagccgatt aattacgatg gggttcatgc gataccaata aataaatgcg tcaacaattc
     4081 agcacacgaa acagacctga tatacatggc tctgctaact gcttcgctgc agtttcagtt
     4141 taccggtata tggggaccaa cgctgtttcg tgtgccgtgg caacctggag gtgccaacct
     4201 taaacttcgg gggaatattc tatacacaac gacgggggat gtcgttagcc acgggagatt
     4261 tatctcataa ataattcacg ttgtcgccat aacggcgaca acgtgaacga agatgggcta
     4321 ttaatggatg ccgccaatgc gacttaagcg cacacctgtc ggccattcgc cttcttgctt
     4381 atcgaaactc atccagatag ccgttgcccg accgaccaga ttcgcttcag gcacaaaacc
     4441 ccagtaacgg ctgtccgcgc tgttgtcgcg gttgtcgccc atcatgaagt attgtcccgg
     4501 cggaacaatc caggttgcca gttgttgccc tggctgctgg taatacatcc ccacctggtc
     4561 ctgcgcaatc ggcactgtca gaattcggtg cgtcacatca cccagtgtct ctttacgctc
     4621 ggaaagacga attccatttt ctttggtttc gtttttcggc acttcaaaga atccgctggt
     4681 cgcttcccca ccattacggc gtgagaaggt ctgaacgaaa tcgctcggtt ccacgttgga
     4741 gtaggtgacc ggtagcgcgt tttcacacgc ctggccggaa ctgcatcccg gttgaatcgt
     4801 cagctctttt gagaccggat cgtaagtgac tttatcgccc ggtaaaccca ccgcgcgctt
     4861 gatgtaatca agctttggat cttccggata tttaaagacc acgatatcgc cgcgtttcgg
     4921 atgaccggtt tcgatcagcg ttttctggta gataggatct ttaatgccat aagcaaactt
     4981 ctctaccaga ataaaatcac cgattaacag agtcggcatc atcgaacctg acgggatctg
     5041 gaacggttca taaataaacg aacgtacaat caatacgata gccagcaccg gaaaaacaga
     5101 agcaccggtt tccagccagc caggcttcgg cgcaaccttt ttcagggttg ctttatccag
     5161 tgagtcaccg gcagccgcct gcgccgctgc ctgacgttcc cgccgtttag gtgcgaaaaa
     5221 gaatttatcc acgcaccata aaatgcccgt caccagtgtg gcaatcacca gaatcagggc
     5281 aaacatattc gccatgccaa ctcctaaggg ttatttgttg tctttgccga cgtgcagaat
     5341 ggcgaggaac gcttcctgcg gcagctcgac gttaccgatc tgcttcatgc gtttcttacc
     5401 ttctttctgc ttctgcagca gctttttctt acggctgata tcgccgccat aacatttagc
     5461 cagtacgttt ttacgcagct gtttcacggt ggatcgcgca ataatgtgcg taccaatcgc
     5521 tgcctgaatg gcgatatcaa actgctggcg tgggatcaga tctttcatct tctccaccaa
     5581 ctcgcgaccg cggttttgcg aattatcacg gtgggtgatc agcgccaacg catcaacacg
     5641 ttcaccgttg attaatacgt ctacacgtac catgtcggac gcctggaagc gtttgaagtt
     5701 gtaatccaga gacgcataac cacgcgaggt agatttcagg cgatcgaaga agtcgagcac
     5761 cacttccgcc atcgggatct cgtacgtcag cgccacctga ttaccgtggt aaaccatatt
     5821 ggtctgcacg ccgcgttttt ctacgcacag cgtaataacg ttgccgagat atgcctgcgg
     5881 cagcagcatg tgacactctg caatcggctc gcgcagttcg taaatgttat ttaccgcagg
     5941 cagcttggat gggctgtcga cgtagataac ttcccttgac gtggtttcaa cttcatacac
     6001 tacggtcggc gcagtggtga tcagatccag atcgtattca cgttccagac gttcctggat
     6061 gatctccatg tgcagcaggc caaggaagcc gcagcggaaa ccaaagccca gcgcgctgga
     6121 gctttccggc tcatagaaca gtgaggcatc attcaggctg agtttaccca gcgcgtcacg
     6181 gaaggcttca tagtcgtcgg aacttaccgg gaacagaccg gcgtatacct gcggtttgac
     6241 tttcttaaag ccaggcagcg ccttttctgc cggattacgc gccagcgtta aggtatcgcc
     6301 gactggagcg ccgtggatat ctttaatcgc acataccagc cagcctactt cgccacattt
     6361 cagttcagtg cggtcaacct gtttcggcgt gaagatgccc agacggtcgg cgttataggt
     6421 ctgcccggta ctcatgactt tcactttgtc gcccttacgc agggtgccgt ttttaatacg
     6481 gataagtgaa acaacgccca ggtagttgtc gaaccatgag tcgataatta gtgcctgcaa
     6541 cgggccttcc ggatcgcctt ccggcggcgg aatgtcgcgc accagacgtt cgagaacgtc
     6601 ctgcacgcca acgccggttt tcgctgaaca gcgcaccgcg tcagtggcgt cgatgccgac
     6661 gatatcttca atttcttccg ccacgcgttc aggatcggct gccggcaggt caatcttgtt
     6721 cagtactggc accacttcga gatccatttc catggcggtg tagcagtttg ccagggtttg
     6781 cgcttccacg ccctgcccgg cgtcgaccac cagcaatgca ccttcacagg cagccagcga
     6841 acgggaaact tcataggaga agtctacgtg gcccggggtg tcgataaagt taagctgata
     6901 ggtttcgccg tcagacgctt tgtagtccag cgtcacgctt tgcgctttga tggtaatgcc
     6961 acgctcacgc tcaagatcca tggaatcgag aacctgcgcc tccatttcac ggtcagacag
     7021 gccaccgcag atctggataa tacggtcaga cagcgtcgat ttaccgtggt caatgtgagc
     7081 tatgatcgaa aagttacgta tattcttcat aaagtatgat tattgtgcct taatgcccgg
     7141 ttaaccaggc ttttagaagt cgctgttctg agcttaacgt ctgtattaat agaaacgccg
     7201 cattctacac tacaacattg aggcgaggaa atgttcatac cgtatggatt gtggtatctg
     7261 gaaacgtcct cgcatttgtt atgcaaaatg caacaaagcc agtgaaatca ctggctcgcg
     7321 tcttccgaag atgtttcaaa tcgcacaagg ccaggcggca ataccacgct taagatgatc
     7381 ggttgccatt ctgcccgggc agcaaactta cgcgagtagc cgcgcgcaat caggaacccg
     7441 ccaataccgc cgaggaccgc accacataat gctgcgacgt cagaagcaaa gagtagctga
     7501 aatagcgaag cgatgaggaa taatcccacc agcggcgaca tataaaccag taatgcggag
     7561 ctaagcaggc tgccttcggc gatccccaat tccacttttt gccccggcac taacggctca
     7621 tcacagggta cgacaatggt atgcgtggtt tgcgggccaa gtttatttaa cacgcggcta
     7681 ccgcaaccgg cgcgtgaagc gcagctgctg catgaagctt taacatcaca actgaccagc
     7741 gcctgcccgt tttgccagga gacgacggta gcccactctt tgatcattgc gctgccccga
     7801 acttaatatt ctcggcaatg cgtttcgccg tttgcggcgg cagttcaccg acaatggtga
     7861 tttcggcgtt atcacgtacg cttgtactga cggttctgcg tccggtgcgc aacatctgat
     7921 cggtgctcga tggcgtagcg cggttaacgt ttaccgagaa gctgaataat ccgtcggaat
     7981 agagacgtga ttcgataggc atgttgtcca tcgtcggtag cggacgtcga ctactggaaa
     8041 cttcgctaaa accctgtggc aaccaggttg gcgtccagct gaatttagct ttttcaccta
     8101 caggaacaga aagcaacggc ggcaaatttg ccttcgccag cgtctgcata ctgctgctga
     8161 tatcctgatt gacgttaaaa gcaatcacgc gaaattgttc cagcgtttca ccatcgcgat
     8221 caaggagatc aacccgcatc ggtaatttcg attcggtgtc catccacacg atgtagctgt
     8281 agcgtgtacc atctcgggca accacgcgaa tgacttcgca aagacgatca gcaatacgcg
     8341 tgcgcccgac ggagataaag tcgtagtaag gagaaaggcg tttgaaatcg gtatagataa
     8401 gcgatggcag agaatcaaca atgtaatcgc cattaagcgt gaacggttca agtcccggtt
     8461 caaaatagct gatttcattg ccgcgctgta ccacttcccg gcgcgggcca tccatttgca
     8521 acaattgtgc aagaggacgg ttatcgaggc gtgcatgtcg ataacgcaga gactcaacac
     8581 cctgtttatt gatgctgatg aatgacagct cgtaattcag tgactgactg gccaggttca
     8641 tctgctgtaa taacgccccg gacgcgggag tggccgaggc gttagcagag aataacaggc
     8701 tacctgtcac taatgacatg gcaaaccaaa gttgcttcat tactgcgatt gcgttcctaa
     8761 agtttgaatt cctggcacct gtacagcggc ttgctgggtt tgcgcctgct caaactgaag
     8821 ctgttcagag tggagtcggc gttgcagttc gtaatcctgc aacattgcat taatgcgacg
     8881 acgctgctcc tgtacctgct gctgttgacc attgtttgcg gtcgcttcag aaggtacccc
     8941 caggcttacc gggctggctt tacccatcat cggcagtgta ttaaataccg gcgtttcggg
     9001 ctgctgggac gtttcagatt gtccattata gtgctggacg ccaacgataa ctgcaagcga
     9061 tacgcatgct gctacgccca tttgggtaag ctgtgccgcc cacggacgta ctttctgcca
     9121 gaatggcatt ttctgccatt gatgcggcgc aggctgggct tccgggatca atgtcgccgg
     9181 ttgacgtact ggctcttctt caatggcggc catcacgcgt gaagagatat cgaaatggag
     9241 cacctcggga gtatcacccc gcattgaatc acggattaag tgatagcttt cccaggtttt
     9301 ctgcatttct gggttatgag ccagttcgtt aagcagctca ctatccagcg tttcgccatc
     9361 cattaaagcg gaaagttgtt ctttctgcat gcctaatacc cttatccagt atcccgctat
     9421 cgtcaacgcc tgataagcgg ttgaactttg ttatcaatag cttccctcgc tcggaagata
     9481 cgtgaacgca ccgtacctac cggacagtcc atgatagcgg ctatctcttc atagctcagg
     9541 ccatccagct cccgcaaggt tattgccatg cgcaaatctt ccgggaggga ctcaatagtt
     9601 cggaaaacta tctgtctcag ttcttctgac aacattaagt tctcagggtt cgaaatttct
     9661 ttcaacgcgc cgccactttc gaagttttca gcttcaatgg catccacatc actggaaggt
     9721 ggacgacgcc cctgagcaac caggtaattt ttcgctgtat ttacagcaat ccgatacagc
     9781 catgtataaa aagcgctatc tccccggaac gaatccagcg cacgataggc tttaataaaa
     9841 gcttcttgta ccacatcggg aacatcaccc gacggcacat agcgggaaac cagactcgcc
     9901 actttatgct gatagcgcac taccagtaag ttaaaggctt tctgatctcc cttctggacc
     9961 cgttcaacca ggacctggtc cgttaactgc tcgctcatcc gaggtaaagt ctccccaaac
    10021 caaatttcca cgcgctatcg aaacgccact ccattagctg caatttgagc aagcaaaggg
    10081 ttagagtgtc tcgtttttgt aaagttccgt aacgcatctg tttttgtttg tcatgctgta
    10141 gacggatcat tatctatcat tataagtcta cagaatctga acatcgcatt atctgtgtag
    10201 aaatgcccat ttaactgcct gaagagtaac ccaacggcct ttttatttca ccacctaatc
    10261 ctccaccagc cagtaacttc tctttttctc gccgccctgc atcagcgtgt ttagcaactg
    10321 taacaaatat taaaatagca ggtgtttatt cgcacaacat gatgctatgc tgaccaaacc
    10381 gtgtttagta aattaaacaa agaaaatgaa tactctccct gaacattcat gtgacgtgtt
    10441 gattattggt agcggcgcag ccggactttc actggcgcta cgcctggctg accagcatca
    10501 ggtcatcgtt ctaagtaaag gcccggtaac ggaaggttca acattttatg cccagggcgg
    10561 tattgccgcc gtgtttgatg aaactgacag cattgactcg catgtggaag acacattgat
    10621 tgccggggct ggtatttgcg atcgccatgc agttgaattt gtcgccagca atgcacgatc
    10681 ctgtgtgcaa tggttaattg accagggggt tttgtttgat acccacgttc aaccgaatgg
    10741 cgaagaaagc taccatctga cccgtgaagg tggacatagt caccgtcgta ttcttcatgc
    10801 cgccgacgcc accggtagag aagtacaatc cacgctggtg agcaaggcgc agaaccatcc
    10861 gaatattcgt gtgctggagc gcagcaacgc ggttgatctg attgtctctg acaaaattgg
    10921 cctgccgggc acgcgacggg ttgttggcgc gtgggtatgg aaccgaaata aagaaacggt
    10981 ggaaacctgc cacgcaaaag cagtggtact ggcaaccggc ggtgcgtcga aggtttatca
    11041 gtacaccacc aatccggata tttcttctgg cgatggcatt gctatggcgt ggcgcgcggg
    11101 ctgtcgggtt gccaatctcg aatttaatca gttccaccct accgcgctgt atcacccaca
    11161 ggcacgcaat ttcctgttaa cggaagcact tcgcggcgaa ggcgcttatc tcaagcgccc
    11221 ggatggcacg cgttttatgc ccgattttga tgagcgcggc gaactggccc cgcgcgatat
    11281 tgtcgcccgc gccattgacc atgaaatgaa acgcctcggc gcagattgta tgttcctcga
    11341 catcagccat aagcccgccg attttattcg ccagcatttc ccgatgattt atgaaaaatt
    11401 gctcgggctg gggattgatc tcacacaaga accggtaccg attgtgcctg ctgcacatta
    11461 tacctgcggt ggtgtaatgg ttgatgatca tgggcgtacg gacgtcgagg gcttgtatgc
    11521 cattggcgag gtgagttata ccggcttaca cggcgctaac cgcatggcct cgaattcatt
    11581 gctggagtgt ctggtctatg gctggtcggc ggcagaagat atcaccagac gtatgcctga
    11641 tgcccacggc gtcagtacgt taccgccgtg ggatgaaagc cgcgttgaga accctgacga
    11701 acgggtagta attcagcata actggcacga gctacggctg tttatgtggg attacgttgg
    11761 cattgtgcgc acaacgaagc gcctggaacg cgccctgcgg cggataacca tgctccaaca
    11821 agaaatagac gaatattacg cccatttccg cgtctcaaat aatttgctgg agctgcgtaa
    11881 tctggtacag gttgccgagt tgattgttcg ctgcgcgatg atgcgcaaag aaagccgggg
    11941 gctgcatttt acgctggatt acccggaact gctcacgcat tccggtccat cgatcctttc
    12001 ccccggcaat cattacataa acagataaaa agcctgggtc agcgccgtat acgcttcgga
    12061 atagttctgg tctggcccac gaatgactaa gcgatcgcta aagcattctc ccgcctgcgg
    12121 ggagaatgcc agcagcaccc gatgcggcag tcgcgcttcg ttttccgcca catccgtccg
    12181 caaacgtaaa tgccagccca tgcttaatgc cagttcagta aaaccattac caatctgctc
    12241 tggcagcacc acgcagaaaa atccctcttc ggtaatgcac tccgccgcac aggtcagcag
    12301 cgatgggtga tcaagcgtag tggtatagcg agcctgttcc cgttgaggtg tcgcgcactc
    12361 tactccctgc tgatagtaag gtgggttact gatgattaaa tcgaagcgta ctgtctgctg
    12421 tgtgatccac tgctgaatat ccgccgtatg gacgttaatc cgctctgccc acggggactg
    12481 gttgatattt tcctgcgcct gcgcggcagc ttcactttcc agctcaacgg catcaattat
    12541 cacgctgtca ctggttcgct gcgccagcat cagtgccagc aacccgctac ccgcgccgat
    12601 atcaaggcaa cgtttcaccc cagccaccgg tgcccatgcg cccagcaaaa tgccatccgt
    12661 tcccactttc atcgcacagc gatcgtgagc aacaaaaaac tgtttaaaag taaatccatt
    12721 acgacgaagc acggatgtag actgtgacat gaaaataaaa ccttgcagga aaaacggcga
    12781 tagcaccggg tgagaacaat acccgagaag cgatatccat acaaacagat gaagattgca
    12841 gccgtaacgt ctataatcag cgccccacac agaggtagaa catgactgta acgacttttt
    12901 ccgaacttga actcgacgaa agcctgctgg aagccctcca ggataaaggt ttcactcgcc
    12961 cgaccgccat tcaggctgcc gccattccgc ctgcgctcga tggccgtgat gtactcggtt
    13021 ctgcgccgac aggcaccggt aaaacggcgg cgtatctgct gccagcgttg cagcacctgc
    13081 tcgatttccc gcgtaagaaa tccggtccgc cgcgtatttt gatcctcacc ccaactcgcg
    13141 agctggcgat gcaggtggcc gatcatgccc gcgaactggc gaaacatacg catctggata
    13201 tcgccaccat caccggcggc gtagcctata tgaaccacgc ggaagtgttc agcgaaaatc
    13261 aggacatcgt ggtcgccacg accggacgtc tgctgcaata cataaaagaa gagaacttcg
    13321 attgccgcgc ggttgaaacg ctgatcctcg acgaagcaga ccgtatgctg gatatgggct
    13381 tcgctcagga tatcgaacat attgctggcg aaacgcgctg gcgtaaacag actctgctct
    13441 tttcggcaac gctggaaggc gatgcgattc aggactttgc cgagcgtctg ctggaagatc
    13501 cggtggaggt ttctgccaat ccgtccaccc gtgagcgcaa aaaaattcat cagtggtatt
    13561 accgcgccga tgacctggaa cataaaaccg cattgctggt gcatctgtta aaacagccgg
    13621 aagcgacccg ctcaattgtg tttgtgcgta agcgtgaacg tgtgcatgag ctggcaaact
    13681 ggctgcgcga agcgggcatc aacaactgct atctcgaagg tgagatggta caaggcaagc
    13741 gtaacgaagc gatcaagcgt ttgaccgaag gtcgcgtaaa cgtactggtt gcaaccgatg
    13801 ttgccgcgcg cggtatcgac attcctgacg tcagccacgt ctttaacttc gatatgccgc
    13861 gcagtggcga tacttatttg caccgtatcg gacgtaccgc gcgcgccggt cgtaaaggca
    13921 ccgcaatttc gctggtggaa gcccatgacc atttgctgct gggcaaggtt ggccgttaca
    13981 ttgaagagcc gattaaagct cgcgttattg atgagttacg cccgaaaacg cgtgcgccaa
    14041 gcgagaagca gaccggcaag ccatcgaaga aagtactggc aaaacgcgcc gagaagaaaa
    14101 aagctaaaga gaaagagaaa ccgcgggtga aaaaacgcca tcgcgacacc aaaaatattg
    14161 gtaagcgccg taaaccaagc ggaacgggcg tgccaccgca aacgacagaa gagtaatctc
    14221 aatgccgggt ttaagcctgg tattaaaagt gctggcaaac gcaaaactgc ctgatgcgct
    14281 acgcttatca ggcctacgtg gctcatgcaa tatattgaat ttgcacgatc ttgtaggccg
    14341 gataaggcgt tcacgccgca tccggcatga ataaagcgca ctttgtcgac aagttaaatg
    14401 ccgggtttaa tcccgacatt atttttatcc agccacaaaa ctctcttcaa cacactgaat
    14461 aaaagtgtgc atcgccgggc taaccgcttt tccagcatgg tgtgcgcaca tggccgtaat
    14521 ggtctgcgac tgttcgccaa aaggcaattc aattaattca ccgcactcca actcttttgc
    14581 caccgcaaaa cgcggcaggt agctgacccc gatatttgcc gcgacacaac gcttgatgct
    14641 ttcgatactt ataagctcaa tggtgttttc caccgtgatc cgccgctgac gcagcgtgct
    14701 ctcaaatatc tgccggaaga cacattgtgg ttcattgata ataaagctac aggcgttatg
    14761 tcttcccggc tcagtaaaat cgacatctac aatttgtggt gaagccacca gcaccagcga
    14821 ttgttcaccc aactctcgtc ggttcagggc atcatcattc cctacgcgat aaaagacgcc
    14881 gacatcggcc tcatcattca gtagcgcatc acggatcacg taacagttca gcgactgcaa
    14941 cgataaacgc acttttggtg cgcgttgccg aaaacgctgc aacacctgcg gcattcggta
    15001 agagagcaac gtttcgcccg aaacaacgcg tagttccccg tccggatctg actccttttt
    15061 ggcagcctca cgaagcgtat ccatcactcg ggtaagttca taaatgtgcg gcagcaactt
    15121 ttttccttcg cgggtaaggc acatccgtcg gccaattttc tcaaataact ggactgaaaa
    15181 ctcctgctca agctgctgaa tatgaaaagt caccgtcgat tgtgtacagc acaatttttg
    15241 cgaagctcgc aaaaaggaac cctcttccac cacggtttta agcgtaataa aacggcgcag
    15301 atccataacc tcaaacctat cgaaaatatc gaatctagaa tataaaaaca ttcatttttt
    15361 taaatgttcc gtgtcgggta ctgtctacca aaacagagga gataacaagt gacaccgacc
    15421 cttttaagtg ctttttggac ttacaccctg attaccgcta tgacgccagg accgaacaat
    15481 attctcgccc ttagctctgc tacgtcgcat ggatttcgtc aaagtacccg cgtgctggca
    15541 gggatgagtc tgggattttt gattgtgatg ttactgtgtg cgggcatttc attttcactg
    15601 gcagtgattg acccggcagc ggtacacctt ttgagttggg cgggggcggc atatattgtc
    15661 tggctggcgt ggaaaatcgc caccagccca acaaaggaag acggacttca ggcaaaacca
    15721 atcagctttt gggccagctt tgctttgcag tttgtgaacg tcaaaatcat tttgtacggt
    15781 gttacggcac tgtcgacgtt tgttctgccg caaacacagg cgctaagctg ggtagttggc
    15841 gtcagcgttt tgctggcgat gattgggacg tttggcaatg tgtgctgggc gctggcgggg
    15901 catctgtttc agcgattgtt tcgccagtat ggtcgccagt taaatatcgt gcttgccctg
    15961 ttgctggtct attgcgcggt acgcattttc tattaacgaa aaaaagcgga agaggtcgcc
    16021 ctcttccgct tagtaacttg ctacttaagc cttacaggct ttcagtaaag gtacgagcga
    16081 taacgtcgcg ctgctgttcc ggagtcagag agttaaagcg aactgcataa ccggatacac
    16141 ggatggtcag ctgcggatat ttttccggat gcttaactgc atcttccaga gtttcacgac
    16201 gcagaacgtt aacgttcagg tgttgaccac cttcaacgcg aacttctggt ttcacttcta
    16261 ctggaacttc acggtattca atgtcaccca gtttgcttac tgcaaccact tcatcttctg
    16321 cataacctgc ttttgcaacg atgcaacgcg cttcgccttt ttcgctgtcc agcagccaga
    16381 aagagttcag cagatcgtcg ttagcggctt tagtaatctg gatacctgta atcatgtgat
    16441 gcctccccgg caaaattatt tgatttgttc agcctgtcgc ggccaattgg taaaaccatt
    16501 gttgcttgag tgtatatact cctcaaacac ccttgaatct ttgatttaaa tcaataaaaa
    16561 ccacacatca agtatggtcg caaatggatt ttattgtttt acatcaactt atgcgggtgt
    16621 gaaattttac caatttacat ttttttgcac tcgtttaagt ctaaaaaatg agcatgattt
    16681 tgttctgtag aaataagcag ttaagctagg cggattgaag attcgcagga gagcgagatg
    16741 gctaacgaat taacctggca tgacgtgctg gctgaagaga agcagcaacc ctattttctt
    16801 aatacccttc agaccgtcgc cagcgagcgg cagtctggcg tcactatcta tccgccacaa
    16861 aaagatgtct ttaacgcgtt ccgctttacc gagttgggtg acgttaaagt ggtgattctc
    16921 ggccaggatc cttatcacgg accgggacag gcgcatgggc tggcattttc cgttcgcccc
    16981 ggaatcgcca ctcctccgtc actgctgaat atgtataaag agctggaaaa tactattccg
    17041 ggcttcaccc gccctaatca tggttatctt gaaagctggg cgcgtcaggg cgttctgcta
    17101 ctcaatactg tgttgacggt acgcgcaggt caggcgcatt cccacgccag cctcggctgg
    17161 gaaaccttca ccgataaagt gatcagcctg attaaccagc atcgcgaagg cgtggtgttt
    17221 ttgttgtggg gatcgcatgc gcaaaagaaa ggggcgatta tagataagca acgccatcac
    17281 gtactgaaag cgccgcatcc atcaccgctt tcggcgcatc gaggattctt tggctgtaac
    17341 cattttgtgc tggcaaatca gtggctggaa caacatggcg agacgccgat tgactggatg
    17401 ccagtattac cggcagagag tgagtaaatt tgcgggaaaa ggccggatgg cagagttgcc
    17461 acccggctga ttcatcaggc tttattctga cgccaccatt cgccaagaag aacgccagtt
    17521 gcaacagaga tattcagccc ggcaacgttg ccagtaccat caatcttcac gcgcaaatcg
    17581 ttcggatcgc gtgcggcatc cggtaacccc tcatattcct gacccagcac cagtaccatt
    17641 ttcgctggca gactggtttt gaacaacggt ttaccctgct cgctggaagt ggtcactacg
    17701 gtgtaacctg cctgacggaa atcatccagc acgttaacaa tgttgtcgcc agtaatcggc
    17761 tgaacgtgct ctgcgccgcc ttctgcggta cggatagccg cccccgactc cagcagtgcc
    17821 gcatcctgca ccacaacgcc tttcacgccg aagtgcgcgc agctgcgcat catgccaccc
    17881 aggttatgcg ggttagattc gttttccagc gccagtacac aatcctgcgc gcctgcctgg
    17941 cttacccact gctgcacggt tgtaccgtta cgctttttga tcaagaagca aacgccgccg
    18001 tgatgttccg tgcctgacgc ttttgtcaat tccgcttcat ccaccacatg gtacgctttg
    18061 cggtttgctg ccatccagcg caaggcttct ttaaaacgcg gcgttacact ctggataaac
    18121 caggcgcgaa caatcgcttc cggacggctc tggaaaagtg cctgacatgc attttcgccg
    18181 tagacgcggg tttcttccgc acgctgacga cgcaacactt ccggatcaat aaaactttta
    18241 ccactgatgc caccgtgatc ggccttttcc ggcgtctcat cacccggcgc gcgggaaacc
    18301 gtgcgccacg gcgaatcttc ccacttgcgg tcacggggct gactctgttt gtcatcgcgg
    18361 gcggggcgac ggccaccgtc agcacgagat tttcctggac gcccgccccc tttcccggta
    18421 cgcaggttgt gggtacgttt atcagaatca tcatcactgc ggacatacat cactttgacc
    18481 ttgccgcttt tacctttcat ttcatcgttc atgcttttct ccaccagcgc tgcgcgaagc
    18541 gcgcagatta cccgaagtcc gcgcggttcg ccatgatttc gtaccaaagc ctgcgactat
    18601 catacctatt gaataaaaca gattgttgtc tggaacaatg tccccgataa tatgtaacat
    18661 attagaaaca taccggcgtc gttgccgata agtctcctta ctcatcccga ggttagttat
    18721 gaataccgtt tgtacccatt gtcaggccat caatcgcatt cccgacgatc ggatcgaaga
    18781 tgcggcaaaa tgcggacgct gcggtcacga cttgtttgac ggagaggtga ttaatgcgac
    18841 cggtgaaaca ctcgacaaat tgctgaagga tgatctacct gtggtgatcg acttctgggc
    18901 accgtggtgc ggcccctgcc gtaatttcgc accaattttt gaagatgtcg cgcaagagcg
    18961 tagcggtaaa gtgcgctttg tgaaagtgaa taccgaagct gaacgtgaat tgagcagtcg
    19021 ctttggaatt cgtagtatac cgacgatcat gattttcaaa aacggtcagg ttgtcgacat
    19081 gcttaatggc gcagtaccga aagcgccgtt cgatagctgg ctgaacgaat ctctttaatc
    19141 ttaccggggc gcatcttgtg ccccgttttc tcctctgcga caatggcgtt ttttcgacgc
    19201 tctcttatga ccgaaaacgc tgttctccag ttacgcgccg agcgtattgc gcgcgcaaca
    19261 cgtccttttc ttgcccgcgg taatcgcgtt cgtcgctgcc aacgctgtct tttgccagag
    19321 aaattatgtc tctgttcgac aattacccca gcacaagcaa aaagtcgctt ctgtttgctg
    19381 atgttcgaca ccgagccaat gaagcccagt aataccgggc gtctgattgc tgatattttg
    19441 cctgataccg ttgcgtttca atggtcgcgt accgaaccct cgcaggattt gctggagttg
    19501 gtgcaaaacc cggactatca gccaatggtg gtctttcccg cttcgtatgc tgatgagcaa
    19561 cgggaagtga tcttcacacc acctgccggt aagccaccgc tgtttatcat gctcgatggt
    19621 acctggccgg aagctcgcaa gatgtttcgt aaaagtccgt atctggataa tcttcccgtc
    19681 atttccgtcg atctttcccg gctttctgcc tatcgcctgc gtgaagccca ggctgaaggc
    19741 caatattgta ctgccgaggt agccatcgca ctgttagata tggccggcga taccggggcg
    19801 gcggcagggt taggcgagca ttttacccgc tttaaaacac gctatctggc aggaaaaacg
    19861 caacatctgg gtagcatcac agcagaacag ttagaaagcg tttaaaatca ttcggtcact
    19921 tctgcgggag accggtatga gtcagcgagg actggaagca ctattgcgac caaaatcgat
    19981 tgcggtaatt ggcgcatcga tgaaacccaa tcgagcaggt tacctgatga tgcgtaacct
    20041 gctggcggga ggctttaacg gaccggtact cccggtgacg cctgcctgga aagcggtgtt
    20101 gggtgtgtta gcctggccgg atattgccag cttgcccttt acacccgacc ttgcggtttt
    20161 atgtaccaat gccagccgta atcttgctct tttggaagag ctcggcgaga aaggctgtaa
    20221 aacctgcatt attctttccg ccccggcatc gcaacacgaa gatctccgcg cctgcgccct
    20281 gcgccataac atgcgcctgc ttggaccaaa cagtctgggt ttactggctc cctggcaagg
    20341 tctgaatgcc agcttttcgc ctgtgccgat taaacgcggc aagctggcgt ttatttcaca
    20401 atcggctgcc gtctccaaca ccatcctcga ctgggcgcaa cagcgtgaga tgggcttttc
    20461 ctactttatt gcgctcggcg acagcctgga tatcgacgtt gatgaattgc ttgactatct
    20521 ggcacgcgac agtaaaacca gcgccatcct gctctatctc gaacagttaa gcgacgcgcg
    20581 acgctttgtt tcggcggccc gtagtgcctc gcgtaataaa ccgattctgg tgattaaaag
    20641 cggacgtagc ccggcggctc aacgtctgtt gaacaccacg gcaggaatgg atccggcgtg
    20701 ggatgcggct attcagcgtg ccggtttgtt gcgggtacag gacacccacg aactgttttc
    20761 ggcggtagaa acccttagcc atatgcgccc gctgcgtggc gaccggttga tgattatcag
    20821 caacggtgct gcgcctgccg cgctggcgct ggatgcctta tggtcacgca atggcaagct
    20881 ggcaacgcta agcgaagaaa cctgccagaa actgcgcgat gcactgccag gacatgtggc
    20941 aatctctaac ccgctcgatc tgcgagatga cgccagcagc gagcattatg tcaaaacgct
    21001 ggacattctg ctccacagcc aggattttga tgcgctgatg gttattcatt cgcccagcgc
    21061 cgctgctccc gcaacagaaa gtgcgcaagc attaattgaa gcggtaaagc atcatccccg
    21121 cagcaagtat gtttctctgc tgacgaactg gtgcggcgag cactcctcgc aagaggcacg
    21181 acgtttattc agcgaagccg ggctgccgac ctaccgtacc ccggaaggaa ccatcactgc
    21241 ttttatgcat atggtggagt accggcgtaa tcagaagcaa ctacgcgaaa cgccggcgtt
    21301 gcccagcaat ctgacttcca ataccgcaaa agcgcatctt ctgttgcaac aggcgattgc
    21361 cgaaggggct acgtcgctcg atacccatga agttcagccc atcctgcaag cgtatggcat
    21421 gaacacgctc cctacctgga ttgccagcga tagcaccgaa gcggtgcata ttgccgaaca
    21481 gattggttat ccgttggcgc tgaaattacg ttcgccggat attccacata aatcggaagt
    21541 tcagggcgtc atgctttacc tgcgtacagc caatgaagtc cagcaagcgg cgaacgctat
    21601 tttcgatcgc gtaaaaatgg cctggccaca ggcgcggatc cacggcctgt tggtgcaaag
    21661 tatggctaac cgtgctggcg ctcaggagtt gcgggttgtg gttgagcacg atccggtttt
    21721 tgggccgttg atcatgctgg gtgaaggcgg tgtggagtgg cgtcctgaag atcaaaccgt
    21781 cgtcgcactg ccgccgctga acatgaacct ggcccgctat ctggttattc aggggatcaa
    21841 aagtaaaaag attcgtgcgc gcagtgcgct acgcccattg gatgttgcag gcttgagcca
    21901 gcttctggtg caggtttcca acttgattgt cgattgcccg gaaattcagc gtctggatat
    21961 tcatcctttg ctggcttctg gcagtgaatt taccgcgctg gatgtcacgc tggatatcgc
    22021 gccgtttgaa ggcgataacg agagtcggct ggcagtgcgc ccttatccgc atcagctgga
    22081 agaatgggta gaattgaaaa acggtgaacg ctgcttgttc cgcccgattt tgccagaaga
    22141 tgagccacaa cttcagcaat tcatttcgcg agtcaccaaa gaagatcttt attaccgcta
    22201 ctttagcgag atcaacgaat ttacccatga agatttagcc aacatgacac agatcgacta
    22261 cgatcgggaa atggcgtttg tagcggtacg acgtattgat caaacggaag agatcctcgg
    22321 cgtcacgcgt gcgatctccg accctgataa catcgatgcc gaatttgccg tgctggttcg
    22381 ctcggatctc aaagggttag gcttaggtcg acgcttaatg gaaaagttga ttacctatac
    22441 gcgagatcac ggactgcaac gtctgaatgg tattacgatg ccaaacaatc gtggcatggt
    22501 ggcgctggcc cgcaagctcg ggtttaacgt tgatatccag ctcgaagagg ggatcgttgg
    22561 gcttacgcta aatcttgccc agcgcgagga atcatgagta aggtactgga aatgttgacc
    22621 acttaatcgg gactggtgtt attattgccc gcttatgtcg tctgcattgc acagaggacc
    22681 cttcaatgaa cagagaagaa atgcactgtg atgttgtcaa aatttaagcg taataaacat
    22741 caacaacacc ttgcccaact acccaagatt tctcaatcag ttgatgatgt cgatttcttt
    22801 tacgctcccg ccgacttccg ggagacgctg ctggaaaaaa tagccagcgc gaagcagcgc
    22861 atttgcattg tcgccctgta tctcgaacag gatgacggtg gcaaaggcat tctgaacgcg
    22921 ttgtatgatg ctaaaaggca gcgtccggaa ctggatgtgc gggtgctggt cgactggcat
    22981 cgtgcacaac gtggacgcat tggcgctgcg gcatctaaca ctaacgctga ctggtactgc
    23041 cgcatggcgc aggaaaatcc gggcgtagat gttccggttt atggcgttcc aatcaatacc
    23101 cgtgaagctc ttggtgttct gcactttaaa ggctttatca tcgacgatag cgtactttat
    23161 agcggtgcca gcctgaacga tgtttacctg catcagcacg ataaatatcg ctacgaccgt
    23221 tatcatctga tccgtaaccg taagatgtca gacattatgt ttgaatgggt tacacagaat
    23281 attatgaatg gccgcggcgt taatcgtctg gatgatgtta atcggccaaa aagcccggaa
    23341 atcaagaacg atattcgtct gttccgccag gagctgcgtg atgccgctta tcatttccag
    23401 ggcgatgccg acaacgatca gctttccgta acgccgctag tggggctggg gaaatcgagt
    23461 ctgttgaaca agaccatttt ccatcttatg ccttgtgccg agcagaaact aaccatctgt
    23521 acgccatact tcaacctgcc agcaatcctt gtgcgcaata ttatccagtt gctgcgcgaa
    23581 gggaaaaagg tcgaaattat tgttggtgat aaaaccgcga acgacttcta cattccggaa
    23641 gatgaacctt tcaagataat tggcgcattg ccttatctct atgagatcaa tctgcgtcgt
    23701 ttcctgagcc gtttgcagta ttacgtcaat actgaccagc tagtggttcg gttatggaaa
    23761 gatgacgaca acacctatca cctgaaaggg atgtgggttg atgataagtg gatgttgatc
    23821 accggtaata acctgaaccc gcgcgcctgg cgtctggatc tggaaaacgc cattttgatc
    23881 cacgatccac aactggagct tgcgccgcag cgagagaaag aactggagct gatccgcgag
    23941 cataccacca tcgttaagca ctatcgcgat ctgcaaagta ttgccgatta tccggtgaag
    24001 gttcgtaaac tcatccgccg tttgcgccgt atccgcatcg accgattaat tagccgcatc
    24061 ctgtaatcac aaccccgtcc tgtacggggt ttgttttttg gaggccacgt tttgcgtatt
    24121 ctttttgtct gttcgctatt gttactttct ggatgcagcc atatggctaa cgatagctgg
    24181 agcgggcagg ataaagctca acactttatc gcctcggcga tgctttccgc cgccggaaat
    24241 gaatattcac agcatcaggg gatgagccgg gatcgcagtg ccatgtttgg attgatgttc
    24301 tctgtcagtt tgggggcgtc aaaagagctt tgggatagcc gccccgaagg gagcggctgg
    24361 agctggaagg atttggcctg ggatgtcgcc ggtgcaagca ccggctatac cgtctggcaa
    24421 ctgacccgtc actaaagacg aatccccttc cctttgcgat gtagcatcaa agaaaccaga
    24481 aacgccacca cggccatcaa ggtcacatac cagaagaagg ctgtttccat tcctattgat
    24541 ttcagcgaca acgctacgta ctccgccgaa ccaccaaata tagcattagc gaccgcatat
    24601 gacagaccaa cgcctaatgc gcgaacctgt gccgggaaca tctcagcctg cagtattcca
    24661 ctgattgatg tataaaaact cactatcagc agggcacaca tcaccagacc aaaagcggca
    24721 taaggcgagg aaacgttttg caatgctgag agaataggaa cggtaaaaat ggctgccagc
    24781 gaaccgaaac ataacattga ggtacggcga ccaatcttat ccgacagcgc gccaatgagt
    24841 ggttgaataa gcatgaatac aaacaatgcg gcagtcataa tgccactcgc cacgttggca
    24901 tgcattcccg cagtatttac cagatacttc tgcatataag tggtgaaggt atagaaacaa
    24961 agggagcccg cagcggtaaa accgagaacc atgatgaatg cacggcgatt gcgccataat
    25021 cctttcagag atccagcttc ttttaaagcg cgcgtttctt gttgcgaagt ttcatctaat
    25081 tgacgacgta accacaacgc cacaacagct aacacagctc ctaacgcgaa aggaatacgc
    25141 catccccact ctctgagtgc agcgtcttcc atggtgtgtt gtaaaaccac gacaaccagt
    25201 agggctagca gttgtccgcc gatcaacgtc acatactgaa atgatgcgta aaaacctttg
    25261 cgctcttcaa cggcaacttc actcatatag gtggcgctgg tgccatattc tccgccaacg
    25321 gataatccct gaaataaacg agcgagaagc aataatgccg gagcccacgt acctatagtt
    25381 tcataacctg ggaggcaggc gataaccagc gatccgaaac acatcataca caccgataac
    25441 agcatcgatt ttttgcgacc atgtttatcg gctatgcggc caaatagcca accgcctatt
    25501 gggcgcatca ggaatcccgc agcaaaaaca cctgctgttt gtagtagttg agtcgtcgtg
    25561 ttcccggaag ggaagaaaat gtgggcgaag tagagtgaac agaacgagta gacatagaaa
    25621 tcgaaccact cgaccagatt acctgaagag gcccccacaa tcgcccaaat gcggcgacga
    25681 gtatcactac ttgtcagttt gctgtctgcc gttacagtac tttcagccat gccattatgt
    25741 ctcctgccgt aatccgatgc ttttgttggt cgcttttgtt tatttctttt ggtaaagaaa
    25801 atgttataca tttgttgcgt atcattatac aaccttaacc atgaatttag ttagcaggaa
    25861 aatggttatt gaggagctta aggataaatt tctggtaagg aggacacgta tggaagtggg
    25921 caagttgggg aaaccgtatc cgttgctgaa tctggcatat gtgggagtat aagacgcgca
    25981 gcgtcgcatc aggcattttt ttctgcgcca atgcaaaaag gccatcctga cggatggcct
    26041 ttcgcttaat ttgatgcctg gcagttccct actctcgcat ggggagaccc cacactacca
    26101 tcggcgctac ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta
    26161 cggccgccag gcaaattctg ttttatcaga ccgcttctgc gttctgattt aatctgtatc
    26221 aggctgaaaa tctttctctc atccgcc
//
LOCUS       DAZKHC020000062        26053 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZKHC020000062 DAZKHC020000000
VERSION     DAZKHC020000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.062"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="ACHOSM_RS14865"
                     /old_locus_tag="pgaptmp_002973"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="ACHOSM_RS14865"
                     /old_locus_tag="pgaptmp_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HGU4559455.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="ACHOSM_RS14870"
                     /old_locus_tag="pgaptmp_002974"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="ACHOSM_RS14870"
                     /old_locus_tag="pgaptmp_002974"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HGU4559456.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="ACHOSM_RS14875"
                     /old_locus_tag="pgaptmp_002975"
     CDS             complement(941..1408)
                     /locus_tag="ACHOSM_RS14875"
                     /old_locus_tag="pgaptmp_002975"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGU4559457.1"
                     /translation="MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHOSM_RS14880"
                     /old_locus_tag="pgaptmp_002976"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHOSM_RS14880"
                     /old_locus_tag="pgaptmp_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGU4559458.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="ACHOSM_RS14885"
                     /old_locus_tag="pgaptmp_002977"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="ACHOSM_RS14885"
                     /old_locus_tag="pgaptmp_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGU4559459.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="ACHOSM_RS14890"
                     /old_locus_tag="pgaptmp_002978"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="ACHOSM_RS14890"
                     /old_locus_tag="pgaptmp_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGU4559460.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="ACHOSM_RS14895"
                     /old_locus_tag="pgaptmp_002979"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="ACHOSM_RS14895"
                     /old_locus_tag="pgaptmp_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGU4559461.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="ACHOSM_RS14900"
                     /old_locus_tag="pgaptmp_002980"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="ACHOSM_RS14900"
                     /old_locus_tag="pgaptmp_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGU4559462.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHOSM_RS14905"
                     /old_locus_tag="pgaptmp_002981"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHOSM_RS14905"
                     /old_locus_tag="pgaptmp_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGU4559463.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="ACHOSM_RS14910"
                     /old_locus_tag="pgaptmp_002982"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="ACHOSM_RS14910"
                     /old_locus_tag="pgaptmp_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGU4559464.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHOSM_RS14915"
                     /old_locus_tag="pgaptmp_002983"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHOSM_RS14915"
                     /old_locus_tag="pgaptmp_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGU4559465.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="ACHOSM_RS14920"
                     /old_locus_tag="pgaptmp_002984"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="ACHOSM_RS14920"
                     /old_locus_tag="pgaptmp_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGU4559466.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHOSM_RS14925"
                     /old_locus_tag="pgaptmp_002985"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHOSM_RS14925"
                     /old_locus_tag="pgaptmp_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGU4559467.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHOSM_RS14930"
                     /old_locus_tag="pgaptmp_002986"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHOSM_RS14930"
                     /old_locus_tag="pgaptmp_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGU4559468.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="ACHOSM_RS14935"
                     /old_locus_tag="pgaptmp_002987"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="ACHOSM_RS14935"
                     /old_locus_tag="pgaptmp_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGU4559469.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="ACHOSM_RS14940"
                     /old_locus_tag="pgaptmp_002988"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="ACHOSM_RS14940"
                     /old_locus_tag="pgaptmp_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGU4559470.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKLIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="ACHOSM_RS14945"
                     /old_locus_tag="pgaptmp_002989"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="ACHOSM_RS14945"
                     /old_locus_tag="pgaptmp_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGU4559471.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHOSM_RS14950"
                     /old_locus_tag="pgaptmp_002990"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHOSM_RS14950"
                     /old_locus_tag="pgaptmp_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGU4559472.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHOSM_RS14955"
                     /old_locus_tag="pgaptmp_002991"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHOSM_RS14955"
                     /old_locus_tag="pgaptmp_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGU4559473.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="ACHOSM_RS14960"
                     /old_locus_tag="pgaptmp_002992"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="ACHOSM_RS14960"
                     /old_locus_tag="pgaptmp_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGU4559474.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="ACHOSM_RS14965"
                     /old_locus_tag="pgaptmp_002993"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="ACHOSM_RS14965"
                     /old_locus_tag="pgaptmp_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGU4559475.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHOSM_RS14970"
                     /old_locus_tag="pgaptmp_002994"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHOSM_RS14970"
                     /old_locus_tag="pgaptmp_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGU4559476.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHOSM_RS14975"
                     /old_locus_tag="pgaptmp_002995"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHOSM_RS14975"
                     /old_locus_tag="pgaptmp_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGU4559477.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="ACHOSM_RS14980"
                     /old_locus_tag="pgaptmp_002996"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="ACHOSM_RS14980"
                     /old_locus_tag="pgaptmp_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGU4559478.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="ACHOSM_RS14985"
                     /old_locus_tag="pgaptmp_002997"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="ACHOSM_RS14985"
                     /old_locus_tag="pgaptmp_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGU4559479.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHOSM_RS14990"
                     /old_locus_tag="pgaptmp_002998"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHOSM_RS14990"
                     /old_locus_tag="pgaptmp_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGU4559480.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHOSM_RS14995"
                     /old_locus_tag="pgaptmp_002999"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHOSM_RS14995"
                     /old_locus_tag="pgaptmp_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGU4559481.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHOSM_RS15000"
                     /old_locus_tag="pgaptmp_003000"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHOSM_RS15000"
                     /old_locus_tag="pgaptmp_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGU4559482.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHOSM_RS15005"
                     /old_locus_tag="pgaptmp_003001"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHOSM_RS15005"
                     /old_locus_tag="pgaptmp_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGU4559483.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHOSM_RS15010"
                     /old_locus_tag="pgaptmp_003002"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHOSM_RS15010"
                     /old_locus_tag="pgaptmp_003002"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGU4559484.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHOSM_RS15015"
                     /old_locus_tag="pgaptmp_003003"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHOSM_RS15015"
                     /old_locus_tag="pgaptmp_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGU4559485.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="ACHOSM_RS15020"
                     /old_locus_tag="pgaptmp_003004"
     CDS             15548..15916
                     /locus_tag="ACHOSM_RS15020"
                     /old_locus_tag="pgaptmp_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HGU4559486.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="ACHOSM_RS15025"
                     /old_locus_tag="pgaptmp_003005"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="ACHOSM_RS15025"
                     /old_locus_tag="pgaptmp_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HGU4559487.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="ACHOSM_RS15030"
                     /old_locus_tag="pgaptmp_003006"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="ACHOSM_RS15030"
                     /old_locus_tag="pgaptmp_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HGU4559488.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHOSM_RS15035"
                     /old_locus_tag="pgaptmp_003007"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHOSM_RS15035"
                     /old_locus_tag="pgaptmp_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGU4559489.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHOSM_RS15040"
                     /old_locus_tag="pgaptmp_003008"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHOSM_RS15040"
                     /old_locus_tag="pgaptmp_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGU4559490.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHOSM_RS15045"
                     /old_locus_tag="pgaptmp_003009"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHOSM_RS15045"
                     /old_locus_tag="pgaptmp_003009"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGU4559491.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDHILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHOSM_RS15050"
                     /old_locus_tag="pgaptmp_003010"
     CDS             complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHOSM_RS15050"
                     /old_locus_tag="pgaptmp_003010"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGU4559492.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHOSM_RS15055"
                     /old_locus_tag="pgaptmp_003011"
     CDS             complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHOSM_RS15055"
                     /old_locus_tag="pgaptmp_003011"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGU4559493.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22621
                     /gene="dprA"
                     /locus_tag="ACHOSM_RS15060"
                     /old_locus_tag="pgaptmp_003012"
     CDS             21497..22621
                     /gene="dprA"
                     /locus_tag="ACHOSM_RS15060"
                     /old_locus_tag="pgaptmp_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HGU4559494.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVIALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22593..23066
                     /gene="smg"
                     /locus_tag="ACHOSM_RS15065"
                     /old_locus_tag="pgaptmp_003013"
     CDS             22593..23066
                     /gene="smg"
                     /locus_tag="ACHOSM_RS15065"
                     /old_locus_tag="pgaptmp_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HGU4559495.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23095..23637
                     /locus_tag="ACHOSM_RS15070"
                     /old_locus_tag="pgaptmp_003014"
     CDS             23095..23637
                     /locus_tag="ACHOSM_RS15070"
                     /old_locus_tag="pgaptmp_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HGU4559496.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23642..24214
                     /gene="tsaC"
                     /locus_tag="ACHOSM_RS15075"
                     /old_locus_tag="pgaptmp_003015"
     CDS             23642..24214
                     /gene="tsaC"
                     /locus_tag="ACHOSM_RS15075"
                     /old_locus_tag="pgaptmp_003015"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HGU4559497.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24219..25037
                     /gene="aroE"
                     /locus_tag="ACHOSM_RS15080"
                     /old_locus_tag="pgaptmp_003016"
     CDS             24219..25037
                     /gene="aroE"
                     /locus_tag="ACHOSM_RS15080"
                     /old_locus_tag="pgaptmp_003016"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGU4559498.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25034..25291
                     /locus_tag="ACHOSM_RS15085"
                     /old_locus_tag="pgaptmp_003017"
     CDS             25034..25291
                     /locus_tag="ACHOSM_RS15085"
                     /old_locus_tag="pgaptmp_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HGU4559499.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25267..25821)
                     /locus_tag="ACHOSM_RS15090"
                     /old_locus_tag="pgaptmp_003018"
     CDS             complement(25267..25821)
                     /locus_tag="ACHOSM_RS15090"
                     /old_locus_tag="pgaptmp_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HGU4559500.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSAKNYVKWKDEYLDQGNQTQP"
BASE COUNT         6687 a         6185 c         6749 g         6432 t
ORIGIN      
        1 gctaaagttc tgggctaatt acgcgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tatcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagtgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gccagcaaac
      961 tatcccaacc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caacaactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacctgagag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccata aaaaaagtag gacagataaa caagcgtaca
     1381 aaatcagaaa gggaagggta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaagct cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggtaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttataaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgtgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgtttcg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtgggg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gctacggtac tttggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgta aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgccagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatttatg tggttacttg accagtgggc agagcgccat atagcagaag cgcaagcgaa
    15601 aggtgagttt gataacctgg caggtagcgg cgaaccattg atactggatg atgattctca
    15661 cgtgccaccg gaattacgtg cggggtatcg cttgctgaag aatgccggtt gcttaccgcc
    15721 agaacttgag caacggagag aagcaattca gcttctggat attctcaaag gtatccgtca
    15781 cgatgatccg caatatcaag aggttagccg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ttgagtaccg attttttacg cggcgattat gctgacaagt tgttggacaa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    15961 aagtaacacc tgacacgatt cgttattacg aaaaacagca gatgatggag catgaagtgc
    16021 gtactgaagg cgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16081 gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgca
    16141 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgctc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actgacagga ttacactcgc
    16381 gccgttaaat aaccaactgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg agtagagaaa
    16501 aataagaaag ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gtcttctgct ttcaggcgct
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgccag taatacttct
    16681 tctttagttg gtgcaggtgc ggcagctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggcc acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt taaagtcgat cccgccaatt aataagccca gaggtggcat gatgatatcg
    16921 gcaaccagtg aagatacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgct catgttattc
    17041 tccctatgaa gccgacaacc ataagtctaa caaatgttaa gccattttcc tgccaggaat
    17101 gaatttaatt aatccttgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaagggct tggctggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacgtcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttcg
    17341 tcaatgactc tgccgacaac gcgtgaggtg ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg caatgaggaa acaccaacaa tatctgcttt tcgcacatgg
    17461 ctaagcaacg cagaaatagt tgcttgttgt ggtgaaattg caatatcgat cacgctcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcaa gcatggcgga catgatattg gcctcgtcat cgttggtgac ggcaataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgctgctg attacgttcg
    17761 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcgcggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc aacaatggtc
    17941 gaaccttgtg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcattaccaa tcagcgggcc gccataatag
    18061 gctttaacta cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgcct
    18121 ggatactcaa tcagtcggta aatattatcg ataactaact gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgtacgta gtctggtgag
    18241 cggatgcgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact ggttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag taatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtctcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggcac caggcagatt ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cggtacgttg caaaaaagct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatccggat ggcgacgaat cacccccgtt gctgaacaag gcgcatctaa taaaatgtga
    18901 tcaaactgtt gctcgccaca ccattgggaa gggtaacggc catcaccttg tttcacggtc
    18961 gccttcatac caaggcgttt taaattgtcg tgaacgcgag agaggcgctg ttcgtcaata
    19021 tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcggcac aaagatccag aatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa
    19141 ccttgtgctg atgcatcctg aacggtaacc catccgtctt caaaaccagg tagcgcatga
    19201 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccaacaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttattg gcttcgacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgctt cagcaaccag gaaggatgca gataacgtgc atcactggta
    19441 ttaaactcgg ctaataactc ttcttgctga cgctggaact ggcgtaatac accgttaatc
    19501 aaccctttaa gttgcggacg cttaattgcg atagcgcctt caaccgtttc agccagcgca
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacaaac caaccataat caggtaatgc
    19621 acagtgcgct gtttgccggt catcggacgg gccattaact tattaatcag ccagtctaac
    19681 tgcgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatca
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgact
    19801 tgttcaacgg cctgggccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
    19861 atgcccggta agaccgggct tagaagagtg gactatcagg ccagacggtt gcccggaaca
    19921 aaccattccc gacgagaatt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
    19981 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttgttg
    20041 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga cgctttccag
    20101 actttaaccg gttgtccttc aatttccagc cagctcattg gccatgggtt gaaagcgcga
    20161 atgcagcgtt caagctgcgc tgccgaaagc gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttctcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gctagttgtt tcaacgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agcgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttataaag catgtcaccg
    20401 gtgtctaaac caacatccat ttgcataatg gtcacaccag tttctgcatc acccgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcact gctttaggca gaattaagcc ataggcgacg
    20581 acgaccataa catcagcctg cagatcggcg accagttgct ggctttcttg tggacgcagg
    20641 gaaacaggtt gaaaaacggg cagacctttt tcctcagcca gaactttaac cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ctagtttttc aactttctga cgaatacgtt gttgtttcag cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agcccctctg
    21001 cttccagttc aaatggttta ccgtcgcggt caagggcgcg aattttaact ttctctgcgc
    21061 gcggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat caacctgggt tgccgccagg ccaatacctt
    21241 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgaatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gcatcagcag
    21541 cttgtacggc gatgatatgg tccgtatcgc tcactggctg gcaaaacagt cgcatattga
    21601 tgcggttgta ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccacgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat
    21721 ccctgcggac agcgaatttt atcctcctca acttcaggcg acgacagatt accccggcgc
    21781 actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgccg tagtggggag
    21841 tcgggcgcat tcatggtatg gcgagcgatg ggggcgatta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacga gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 tgccttacag gtaaatggcg tcagcattgc tgtattgggg aacggactta ataccattca
    22021 tccccgccgc catgcccgac tggctaccag tttgcttgaa catggtgggg cacttgtctc
    22081 ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgttggt
    22201 gacagcacgt tgtgcgcttg agcaggggcg tgaagttatt gccttgccag gaccaatagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
    22621 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
    22681 ttaccgacgc aggatttgat cgagaagata tctacaatgc cctgctttgg ctggaaaagc
    22741 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
    22801 tgcgtattta tacccctgag gagtgtgaaa gactggatgc cagctgtcgt ggttttctgc
    22861 ttttccttga gcaaattcag gtgcttaacc ttgaaacccg tgaaatggtg atagagcgag
    22921 tgctcgcgct ggataccgca gagttcgacc tggaagacct gaaatgggta atcctgatgg
    22981 tgttgttcaa tattccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
    23041 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggcg
    23101 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg
    23161 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac
    23221 tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag
    23281 gtttgccctg catgtggcgc aaatctggta ttacgccagg gacgctttgg tatgtttatt
    23341 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
    23401 gcaattacat gcccccaatg tcggacgggc catctggtcc agcgccgctc ccgttatggc
    23461 aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaaccc
    23521 atagctggag aatgccctga gtgtcattat ccgctactca tcgaaaagaa aaccgcgcag
    23581 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa
    23641 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaaa
    23701 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga
    23761 aacagcagtg atgcgactgt tggagttaaa acagcgtccg gttgataagg ggctgatttt
    23821 aatcgcggca aattacgagc agcttaaacc ctatattgat gacaccatgc tgactgatgc
    23881 gcagcgtgaa accatttttt cccgctggcc aggtcctgtc acctttgtct ttcccgcgcc
    23941 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
    24001 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
    24061 caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
    24121 ggcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga
    24181 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
    24241 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct cagcaactga
    24301 atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga
    24361 acgctttctt tagtgctggt ggtaaaggtg cgaatgtgac ggtacctttt aaagaagagg
    24421 cttttgccag agcggatgag cttactgaac gggcagcgtt agctggtgct gttaataccc
    24481 tcaagcggtt agaagatgga cgcctgctgg gtgacaatac cgatggtata ggtttgttaa
    24541 gcgatctgga acgtctgtct tttatccgtc ctggtttacg tattctgctc atcggcgctg
    24601 gtggagcttc tcgcggcgta ctactgccac ttctttccct ggactgtgcg gtgacaatca
    24661 ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta
    24721 ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa
    24781 catccagtgg catcagtggt gatattccgg cgatcccgtc atcgcttatt catccaggca
    24841 tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc
    24901 agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg
    24961 ccttccttct ttggcatggc gttctgcctg atgtagaacc ggtcatcaaa ctattgcaac
    25021 aggaattgtc agcgtgaatc aggccatcca gtttccggat agggaagagt gggacgagaa
    25081 taaaagatgt gtatgttttc ccgctctcgt gaatggtatg caactgacat gtgcgatctc
    25141 tggcgagagt ctggcgtatc gctttactgg agatacgcca gaacagtggt tagcgagttt
    25201 tcgtcagcat cgctgggacc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
    25261 agatgatcaa ggttgggtct ggttaccctg atccagatat tcgtccttcc atttcacgta
    25321 attcttcgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt
    25381 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccaggct
    25441 acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
    25501 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt
    25561 gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac
    25621 atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc
    25681 tccacgaatc acaacgagcg gccagatccc gacatcatca gccagacgaa cgtcaccaat
    25741 cacgacgctg ctatcgtcga tcattacgcg ctggccgatt tgtggaaaaa gatcgcggta
    25801 tgggcgtaaa acatcagaca tactcacctc aacaataaat gatttcctaa tgactttggg
    25861 tgcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgaa
    25921 tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaag tacttgtgca
    25981 aaaaattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacaag
    26041 atggtcgtaa caa
//
LOCUS       DAZKHC020000063        25711 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZKHC020000063 DAZKHC020000000
VERSION     DAZKHC020000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.063"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..89)
                     /locus_tag="ACHOSM_RS15095"
                     /old_locus_tag="pgaptmp_003019"
     rRNA            complement(<1..89)
                     /locus_tag="ACHOSM_RS15095"
                     /old_locus_tag="pgaptmp_003019"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(533..3106)
                     /gene="clpB"
                     /locus_tag="ACHOSM_RS15100"
                     /old_locus_tag="pgaptmp_003020"
     CDS             complement(533..3106)
                     /gene="clpB"
                     /locus_tag="ACHOSM_RS15100"
                     /old_locus_tag="pgaptmp_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HGU4559501.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(3236..3967)
                     /gene="yfiH"
                     /locus_tag="ACHOSM_RS15105"
                     /old_locus_tag="pgaptmp_003021"
     CDS             complement(3236..3967)
                     /gene="yfiH"
                     /locus_tag="ACHOSM_RS15105"
                     /old_locus_tag="pgaptmp_003021"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HGU4559502.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEAAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3964..4944)
                     /gene="rluD"
                     /locus_tag="ACHOSM_RS15110"
                     /old_locus_tag="pgaptmp_003022"
     CDS             complement(3964..4944)
                     /gene="rluD"
                     /locus_tag="ACHOSM_RS15110"
                     /old_locus_tag="pgaptmp_003022"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HGU4559503.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEIFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            5079..5816
                     /gene="bamD"
                     /locus_tag="ACHOSM_RS15115"
                     /old_locus_tag="pgaptmp_003023"
     CDS             5079..5816
                     /gene="bamD"
                     /locus_tag="ACHOSM_RS15115"
                     /old_locus_tag="pgaptmp_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HGU4559504.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            6087..6428
                     /gene="raiA"
                     /locus_tag="ACHOSM_RS15120"
                     /old_locus_tag="pgaptmp_003024"
     CDS             6087..6428
                     /gene="raiA"
                     /locus_tag="ACHOSM_RS15120"
                     /old_locus_tag="pgaptmp_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGU4559505.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6531..6656
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6532..6579
                     /gene="pheL"
                     /locus_tag="ACHOSM_RS15125"
                     /old_locus_tag="pgaptmp_003025"
     CDS             6532..6579
                     /gene="pheL"
                     /locus_tag="ACHOSM_RS15125"
                     /old_locus_tag="pgaptmp_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HGU4559506.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6679..7839
                     /gene="pheA"
                     /locus_tag="ACHOSM_RS15130"
                     /old_locus_tag="pgaptmp_003026"
     CDS             6679..7839
                     /gene="pheA"
                     /locus_tag="ACHOSM_RS15130"
                     /old_locus_tag="pgaptmp_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HGU4559507.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7882..9003)
                     /gene="tyrA"
                     /locus_tag="ACHOSM_RS15135"
                     /old_locus_tag="pgaptmp_003027"
     CDS             complement(7882..9003)
                     /gene="tyrA"
                     /locus_tag="ACHOSM_RS15135"
                     /old_locus_tag="pgaptmp_003027"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HGU4559508.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(9014..10084)
                     /gene="aroF"
                     /locus_tag="ACHOSM_RS15140"
                     /old_locus_tag="pgaptmp_003028"
     CDS             complement(9014..10084)
                     /gene="aroF"
                     /locus_tag="ACHOSM_RS15140"
                     /old_locus_tag="pgaptmp_003028"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HGU4559509.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLHPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10294..10659
                     /gene="yfiL"
                     /locus_tag="ACHOSM_RS15145"
                     /old_locus_tag="pgaptmp_003029"
     CDS             10294..10659
                     /gene="yfiL"
                     /locus_tag="ACHOSM_RS15145"
                     /old_locus_tag="pgaptmp_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HGU4559510.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10809..11327
                     /gene="yfiR"
                     /locus_tag="ACHOSM_RS15150"
                     /old_locus_tag="pgaptmp_003030"
     CDS             10809..11327
                     /gene="yfiR"
                     /locus_tag="ACHOSM_RS15150"
                     /old_locus_tag="pgaptmp_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HGU4559511.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11317..12543
                     /gene="dgcN"
                     /locus_tag="ACHOSM_RS15155"
                     /old_locus_tag="pgaptmp_003031"
     CDS             11317..12543
                     /gene="dgcN"
                     /locus_tag="ACHOSM_RS15155"
                     /old_locus_tag="pgaptmp_003031"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HGU4559512.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRSEKLVR"
     gene            12559..13041
                     /gene="yfiB"
                     /locus_tag="ACHOSM_RS15160"
                     /old_locus_tag="pgaptmp_003032"
     CDS             12559..13041
                     /gene="yfiB"
                     /locus_tag="ACHOSM_RS15160"
                     /old_locus_tag="pgaptmp_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU4559513.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPGSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(13118..13465)
                     /gene="rplS"
                     /locus_tag="ACHOSM_RS15165"
                     /old_locus_tag="pgaptmp_003033"
     CDS             complement(13118..13465)
                     /gene="rplS"
                     /locus_tag="ACHOSM_RS15165"
                     /old_locus_tag="pgaptmp_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGU4559514.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13507..14274)
                     /gene="trmD"
                     /locus_tag="ACHOSM_RS15170"
                     /old_locus_tag="pgaptmp_003034"
     CDS             complement(13507..14274)
                     /gene="trmD"
                     /locus_tag="ACHOSM_RS15170"
                     /old_locus_tag="pgaptmp_003034"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGU4559515.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(14305..14853)
                     /gene="rimM"
                     /locus_tag="ACHOSM_RS15175"
                     /old_locus_tag="pgaptmp_003035"
     CDS             complement(14305..14853)
                     /gene="rimM"
                     /locus_tag="ACHOSM_RS15175"
                     /old_locus_tag="pgaptmp_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGU4559516.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVGTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14872..15120)
                     /gene="rpsP"
                     /locus_tag="ACHOSM_RS15180"
                     /old_locus_tag="pgaptmp_003036"
     CDS             complement(14872..15120)
                     /gene="rpsP"
                     /locus_tag="ACHOSM_RS15180"
                     /old_locus_tag="pgaptmp_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGU4559517.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15257..16618)
                     /gene="ffh"
                     /locus_tag="ACHOSM_RS15185"
                     /old_locus_tag="pgaptmp_003037"
     CDS             complement(15257..16618)
                     /gene="ffh"
                     /locus_tag="ACHOSM_RS15185"
                     /old_locus_tag="pgaptmp_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGU4559518.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16785..17576
                     /gene="ypjD"
                     /locus_tag="ACHOSM_RS15190"
                     /old_locus_tag="pgaptmp_003038"
     CDS             16785..17576
                     /gene="ypjD"
                     /locus_tag="ACHOSM_RS15190"
                     /old_locus_tag="pgaptmp_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HGU4559519.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17597..18883
                     /gene="yfjD"
                     /locus_tag="ACHOSM_RS15195"
                     /old_locus_tag="pgaptmp_003039"
     CDS             17597..18883
                     /gene="yfjD"
                     /locus_tag="ACHOSM_RS15195"
                     /old_locus_tag="pgaptmp_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HGU4559520.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18938..19531)
                     /gene="grpE"
                     /locus_tag="ACHOSM_RS15200"
                     /old_locus_tag="pgaptmp_003040"
     CDS             complement(18938..19531)
                     /gene="grpE"
                     /locus_tag="ACHOSM_RS15200"
                     /old_locus_tag="pgaptmp_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGU4559521.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIQKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19654..20532
                     /gene="nadK"
                     /locus_tag="ACHOSM_RS15205"
                     /old_locus_tag="pgaptmp_003041"
     CDS             19654..20532
                     /gene="nadK"
                     /locus_tag="ACHOSM_RS15205"
                     /old_locus_tag="pgaptmp_003041"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HGU4559522.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20618..22279
                     /gene="recN"
                     /locus_tag="ACHOSM_RS15210"
                     /old_locus_tag="pgaptmp_003042"
     CDS             20618..22279
                     /gene="recN"
                     /locus_tag="ACHOSM_RS15210"
                     /old_locus_tag="pgaptmp_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGU4559523.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22428..22769
                     /gene="bamE"
                     /locus_tag="ACHOSM_RS15215"
                     /old_locus_tag="pgaptmp_003043"
     CDS             22428..22769
                     /gene="bamE"
                     /locus_tag="ACHOSM_RS15215"
                     /old_locus_tag="pgaptmp_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HGU4559524.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22831..23121)
                     /gene="yfjF"
                     /locus_tag="ACHOSM_RS15220"
                     /old_locus_tag="pgaptmp_003044"
     CDS             complement(22831..23121)
                     /gene="yfjF"
                     /locus_tag="ACHOSM_RS15220"
                     /old_locus_tag="pgaptmp_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HGU4559525.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(23111..23548)
                     /gene="ratA"
                     /locus_tag="ACHOSM_RS15225"
                     /old_locus_tag="pgaptmp_003045"
     CDS             complement(23111..23548)
                     /gene="ratA"
                     /locus_tag="ACHOSM_RS15225"
                     /old_locus_tag="pgaptmp_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HGU4559526.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23719..24201
                     /gene="smpB"
                     /locus_tag="ACHOSM_RS15230"
                     /old_locus_tag="pgaptmp_003046"
     CDS             23719..24201
                     /gene="smpB"
                     /locus_tag="ACHOSM_RS15230"
                     /old_locus_tag="pgaptmp_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGU4559527.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPSRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24416..24778
                     /gene="ssrA"
                     /locus_tag="ACHOSM_RS15235"
                     /old_locus_tag="pgaptmp_003047"
     tmRNA           24416..24778
                     /gene="ssrA"
                     /locus_tag="ACHOSM_RS15235"
                     /old_locus_tag="pgaptmp_003047"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            25050..25298
                     /locus_tag="ACHOSM_RS15240"
                     /old_locus_tag="pgaptmp_003048"
     CDS             25050..25298
                     /locus_tag="ACHOSM_RS15240"
                     /old_locus_tag="pgaptmp_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4559528.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(25309..25428)
                     /locus_tag="ACHOSM_RS15245"
                     /old_locus_tag="pgaptmp_003049"
                     /pseudo
     CDS             complement(25309..25428)
                     /locus_tag="ACHOSM_RS15245"
                     /old_locus_tag="pgaptmp_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(25459..>25711)
                     /locus_tag="ACHOSM_RS15250"
                     /old_locus_tag="pgaptmp_003050"
     CDS             complement(25459..>25711)
                     /locus_tag="ACHOSM_RS15250"
                     /old_locus_tag="pgaptmp_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU4559529.1"
                     /translation="AQLQVPESLRTCPVTLCEPEDGVFMRNSMNSNVCMLYDKMALIH
                     LVKTRAAHPLSRESIAVSMIVGRDNCAFDPDRGNFVLKN"
BASE COUNT         6592 a         6740 c         5986 g         6393 t
ORIGIN      
        1 cagcaagctg tttcctgtta ccgttcgact tgcatgtgtt aggcctgccg ccagcgttca
       61 atctgagcca tgatcaaact cttcaattta aaagtttgat gctcaaagaa ttaaacttcg
      121 taatgaatta cgtgttcact cttgagactt ggcattcatt tttcgtcttg cgacgttaag
      181 aatccgtatc ttcgagtgcc cacacagatt gtctgataaa ttgttaaaga gcagtgccgc
      241 ttcgcttttt ctcagcggcg cggggtgtgc ataatacgct ttcccgctac agagtcaagc
      301 atttattttt gctttctctg ccggaattct caggagaacc ccgctgaccc ggcggcgtgt
      361 ttgccgttgt tccgtgtcag tggtggcgca ttatagggag ttattccggc ctgacaagcg
      421 aaaaatataa aaactttatc gttcgctcat ttttcaggca aaacatctta aatatagtct
      481 tttccgtcta acttatagac aaaaacgagc cccgaagggc tcgttttatc atttactgga
      541 cggcgacaat ccggtcttca ttaacttcca ggcgaatcac tttacccgga accaattcac
      601 cagacagtat ttgctgtgcc agcgggtttt cgatctgctg ctggatagca cgtttcaaag
      661 gacgtgcacc atagactgga tcgtaaccgt tctcgctcag cagtttcagc gcctcgtcag
      721 aaatgtggat ttcataacca cgttcttcca gacgtttgta cagacgtttc aactgaatct
      781 gcgcaatcga ggcaatgtgc tgttcaccca gcggatggaa gaccaccact tcatcaatac
      841 ggttaatgaa ttccggacgg aagttatggc ttaccacacc gagcaccagc tctttcatgt
      901 gcgcataatc cagttcaccg aagcgttcct gaatcaaatc ggaaccgagg ttagaggtca
      961 taatgacaac cgtattacgg aagtcgaccg ttctcccctg cccgtcagtc agacgaccat
     1021 catccagtac ctgcaacaga atgttgaaga catccggatg cgctttttcc acttcatcca
     1081 gcaggatgac ggaatacgga cgacgacgca cagcttcggt caggtagcca ccttcttcat
     1141 aaccgacata tcccggaggc gcaccaacca gacgagacac cgagtgtttc tccataaact
     1201 cggacatatc gatacggacc atcgcctcgt cgctatcaaa cataaagttc gccagcgcct
     1261 tacaaagctc agttttcccc acacccgttg ggccgaggaa caggaatgaa ccaatcgggc
     1321 gatttggatc cgccaacccc gcacggctac ggcgaatagc gttagatacc gcatcaaccg
     1381 cttcgttctg accaatgacg cgatggtgca gttcttgctc catacgcagc agtttttcgc
     1441 gctcgctttc catcatgcga gaaaccggaa tccccgtcca acgcgccagc acttcagcaa
     1501 tttcggcgtc ggtcacttta ttacgcaaca gacgcatagt tttgccttcg agctgcgttg
     1561 cggcttccag ttgcttttcc agttccggga ttttgccgta ttgcagttca gacatccgcg
     1621 ccaggtcccc cacacggcga gcctgttcaa tagcgatttt cgcctgttcc agttccgctt
     1681 taatggtctg cgtaccagaa agcgatgcct tctctgcttt ccactcttct tctaactcgg
     1741 agtactgacg ttctttgtcg ctcagttctt cgttgagcat atccaggcgt tttttactgg
     1801 cttcatcaga ctctttcatt aacgcctgtt gttccagttt gagctggatg atacgacgat
     1861 cgagtcggtc gagttcttct ggttttgagt caatctgcat acgaatgctg gatgctgctt
     1921 catcgatcag gtcgatggct ttatccggca gctgacggtc agcaatgtag cgatgagaca
     1981 acgtcgccgc tgcaacaatt gccgggtcag taatttgcac atggtggtgc aattcgtaac
     2041 gttctttcag gccacgcaga atcgcaatgg tatcttcaac agaaggttcg gcaacaaaca
     2101 ctttctggaa acgacgttcc agcgcagcat ctttttcaat gtactggcga tattcgtcaa
     2161 gcgtcgtggc acctacgcag tgcaattcac cacgcgccag cgccggtttc agcatgtttc
     2221 cggcgtccat tgcgccatcg gctttacccg cgccgaccat ggtatgtaat tcgtcgataa
     2281 acaggatgac gttgccttcc tgtttggcaa gatcgttaag cacgcctttt aaacgttctt
     2341 caaactcacc gcgatatttc gccccagcca ccagcgcgcc catatccagc gccagtacgc
     2401 ggcggccttt caatccttcc ggcacttcgc cgttgataat acgctgcgcc agaccttcaa
     2461 cgatggcagt tttaccgacg ccgggttcac caatcagtac cgggttattt ttagtacgac
     2521 gttgcagcac ctgaatggta cggcgaattt cttcatcacg accaatcacc ggatcgagtt
     2581 tgccctgttc ggctcgttcg gtaaggtcga tggtatattt tttcaaagcc tgacgttggt
     2641 cttcagcacc ttgatcgttc acactttcac ctccacgcat ttgttcaatc gcttgagtaa
     2701 tgttggcggt ggtcgcccct gctgctttca ggatgtcggc cagcgtgccg cgagactcaa
     2761 gtgccgccag aacgaacagt tctgacgaga taaagttatc accacgtttt tgcgccagct
     2821 tgtcgcaaag attaagaacg cgcaccagat cctgtgatgg ctggacatca ccaccagtac
     2881 cttcaacctg cggtaaacga tttaatgcct gattgatatc tgtgcgcaac tggccagcat
     2941 ttatgccagc ggatgttaat aaaggactaa ccgaaccccc ttcctgattc agcagggcgc
     3001 tcattaaatg aagtggttcg atgaattggt tgtcgtgccc gagtgcaagt gattgggcat
     3061 cggcaagagc aagctggaat ttattagtaa gacgatccag acgcataact cctcccataa
     3121 cggatcaaag ttgctactgg agattaaatg aggtcatccc tcaattattc aaggttattg
     3181 accagattaa tgtgaaaaga aaatcacgcg taccggatcg tcttgattct ttaggttata
     3241 tcagccaaat gaaacttgcc atacgaccgg tggtcttgtc gcgacgataa gagaagaaag
     3301 tctcattttc cgtatatgta caacggtcgc cgccgaaaat ttgctcaaca cccacgttcg
     3361 ccagacgctg ccgggcaagc tgataaatat ccgccagata cttatcacca tgctgaatga
     3421 aagctgcact tgctttagcg tctactgcca taaacgcctc gcgaacctcc acccccactt
     3481 cgaacgcacg tggaccaatt gccggcccta accaagcgag aatattttcc ggattatcag
     3541 caaaacagga aaccgtctct tccagcacgc ctgcgcacag tccacgccag ccagcatgag
     3601 cggcggcggc ttccgttccc gctcgattgc aaaacagcac agggaggcag tcggcagtca
     3661 tcactgcgca aaccgtgccg ggcgtattgc tataagaggc atctgctcgt tttgaggcat
     3721 aaggttcgcc agtaagctta agcacatctt tgccgtgtac ctgctcaagc cagaccggtt
     3781 tagaaggcaa attgcccgca gcaaaaagtc gcttgcgatt ctcctcaacg tgatccgggt
     3841 tatcgccaca atgggcaccg aggttgagcg agtcatacgg gggcaagctc acgccgccga
     3901 tacgagaaga gctacaggcc gcaacacctt ttggtagcgg ccactgcggg acaatcagct
     3961 tactcataac cagtccactt catccttatg ctcttcgaaa tcggcgcgca tcacctcaat
     4021 cagctccacc atatcttgtg gaataggcgc atgccattcc atttcgatgc cggagatcgg
     4081 gtgataaaga cgcagcatgg ttgcatgaag cgcctggcgg tcaaacttac gcagcatgga
     4141 gataaatgct tccgaagcac cttttggcgg acgcggacgg ccaccataaa ccggatcgcc
     4201 caccagcgga tgagtgatat gggccatatg cacgcggatc tggtgcgtac gtccagtttc
     4261 cagacgcaac cgcagacgcg tgtgcacacg gaagtgttcc atgatgcgat agtgagtcac
     4321 cgccggtttg cccatcggat gcaccgccat gtgagtacgt ttggtcgggt ggcggctgat
     4381 gggttcatct actgtaccgc cagcggtcat atgaccaatt gccaccgctt catactcacg
     4441 agtaatttca cgcctttgca aagattcgac taaacgcgtc tgagccggaa cggtttttgc
     4501 cacaaccatc aggccagtgg tgtctttatc cagacgatgg acgatgcctg cacgcggtac
     4561 atcagcaatg ggcggatagt aatgaagcaa cgcattcagt accgtgccat ccgggttacc
     4621 cgcgccagga tgtaccacca ggtcgcgcgg tttattaatg acaatgatat cttcatcttc
     4681 atagacgata tccagcggga tatcctgcgg ttcaaaacgc gcttcttctt caatctcagc
     4741 gtttatggca acctgctcgc cacccaatac tttttctttc ggcttatcac aaactttgcc
     4801 gtttaccagc acgcgctggt cgaggatcca ttcttttatt cgcgaacgtg aataatccgg
     4861 gaatatttcg gccaaagcct gatctaagcg ttgaccgagt tggttttcgg acaccgttgc
     4921 agtgagctgt actcgttgtg ccatatatac tgcttcttcg tttatcgttg gggttttacg
     4981 gctttgccgt ttaatatagt gtgctattgt agctggtctt aaccgggagc aggaacagag
     5041 aatctcccgt attacatttt gaggaaagtc aaaacgtcat gacgcgcatg aaatatctgg
     5101 tggcagccgc cacactaagc ctgtttttgg cgggttgctc ggggtcaaag gaagaagtac
     5161 ctgataatcc gccaaatgaa atttacgcga ctgcacaaca aaagctgcag gacggtaact
     5221 ggagacaggc aataacgcaa ctggaagcgt tagataatcg ctatccgttt ggtccgtatt
     5281 cgcagcaggt gcagctggat ctcatctacg cctactataa aaacgccgat ttgccgttag
     5341 cacaggctgc catcgatcgt tttattcgcc ttaacccgac ccatccgaat atcgattatg
     5401 tcatgtacat gcgtggcctg accaatatgg cgctggatga cagtgcgctg caagggttct
     5461 ttggcgttga ccgtagcgat cgcgatcctc aacatgcacg agctgcgttt agtgactttt
     5521 ccaaactggt gcgcggctat ccgaacagtc agtacactac cgatgccacc aaacgtctgg
     5581 tattcctgaa agatcgtctg gcgaaatatg aatactccgt tgccgagtac tatacagaac
     5641 gtggtgcatg ggttgccgtc gttaaccgcg tagaaggcat gttgcgcgac tacccggata
     5701 cccaggctac gcgtgatgcg ctgccactga tggaaaatgc ataccgtcag atgcagatga
     5761 atgcgcaagc tgaaaaagta gcgaaaatca tcgccgcaaa cagcagcaat acataacaga
     5821 aacctgaaac acaaaacggc agcccttgag ctgccgtttt tttattctgt tagttgtgaa
     5881 actgaagcga tttagtcact atcgatctca tcaaatatgg ctcgctttga gatattcctc
     5941 aagtaaaaaa acacctcttc ctgcgatttc tcacaaaaaa gattcgttga caaaaagtga
     6001 caaaattatg agattcccat cacacatttt gacatcagga acggtatgct gaattcacca
     6061 agacgggaag acaagaggta aaatttatga caatgaacat taccagcaaa caaatggaaa
     6121 ttactccggc catccgccaa catgtcgcag accgtctcgc caaactggaa aaatggcaaa
     6181 cacatctgat taatccacat atcattctgt ccaaagagcc acaagggttt gttgctgacg
     6241 ccacaatcaa tacacctaac ggcgttctgg ttgccagtgg taaacatgaa gatatgtaca
     6301 ctgcaattaa cgaattgatc aacaagctgg aacggcagct caataaactg cagcacaaag
     6361 gcgaagcacg tcgtgccgca acatcggtga aagacgccaa cttcgtcgaa gaagttgaag
     6421 aagagtagtc ctttatattg agtgtatcgc caacgcgcct tcgggcgcgt tttttgttga
     6481 cagcgtgaaa acagtacggg tactgtacta aagtcactta aggaaacaaa catgaaacac
     6541 acaccgtttt tcttcgcatt cttttttacc ttcccctgaa tgggaggcgt ttcgtcgtgt
     6601 gaaacagaat gcgaagacga acaataaggc ctcccaaacc ggggggcctt ttttattgat
     6661 aacaaaaaag gcaacactat gacatcggaa aatccattac tggcgctgcg agagaaaatc
     6721 agcgcgctgg atgaaaaatt attagcatta ctggcagagc ggcgcgaact ggccgtcgag
     6781 gtgggaaaag ccaaactgct ctcgcatcgc ccggtacgag atattgatcg tgaacgcgat
     6841 ttactggaaa gattaattac gctcggtaaa gcgcaccatc tggacgccca ttacattact
     6901 cgcctgttcc agctcatcat tgaagattcc gtattaactc agcaggcttt gctccagcaa
     6961 catctcaata aaattaatcc gcactcagca cgcatcgctt ttctcggccc caaaggttct
     7021 tattcccatc ttgcggcgcg ccagtatgct gcccgtcact ttgagcaatt cattgaaagt
     7081 ggctgcgcca aatttgccga tatttttaat caggtggaaa ccggccaggc cgactatgcc
     7141 gtcgtaccga ttgaaaatac cagctccggt gccataaacg acgtttacga tctgctgcaa
     7201 cataccagct tgtcgattgt tggcgagatg acgttaacta tcgaccattg tttgttggtc
     7261 tccggcacga ctgatttatc caccattaat acggtctaca gccatccgca gccattccag
     7321 caatgcagca aattccttaa tcgttatcct cactggaaga ttgaatatac cgaaagtacg
     7381 tctgcggcaa tggaaaaggt tgcacaggca aaatcaccgc atgttgctgc gttgggaagc
     7441 gaagctggcg gcactttgta cggtttgcag gtactggagc gtattgaagc gaatcagcaa
     7501 caaaacttca cccgatttgt ggtgttggca cgtaaagcca ttaacgtgtc tgaccaggtt
     7561 ccggcgaaaa cgacgttgtt aatggcgacc gggcaacaag ccggtgcgct ggttgaagcg
     7621 ttgctggtgc tgcgcaacca taatctgatt atgacccgtc tggaatcacg cccgattcac
     7681 ggtaatccat gggaagagat gttttatctg gatattcagg ccaatcttga atcagcggaa
     7741 atgcaaaaag cattgaaaga gttaggggaa attacccgtt caatgaaggt attgggctgt
     7801 tacccaagtg agaacgtagt gcctgttgat ccaacctgat gaaaaggtgc cggatgatgt
     7861 gaatcatccg gcactggatt attactggcg gttgtcattc gcctgacgca ataacacgcg
     7921 gctttcactc tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct tgcggaaact
     7981 gtcaataaac gcctgcttat cgccctgctc cagcaactca atcgcctcgc cgaaacgctt
     8041 atagtaacgt ttgattaacg ccagattacg ctctgacgac ataataatgt cggcataaag
     8101 ctgcggatcc tgagcaaaca gtcgcccgac catcgccagc tcaaggcggt aaatcggcga
     8161 agagagcgcc agaagttgct caagctgaac attttcttct gccagatgca gcccataagc
     8221 aaaagtagca aagtggcgca gagcctgaat aaacgccata ttctgatcgt gctcgacagc
     8281 gctaatacga tgcagccgag cgccccagac ctgaatttgc tccagaaacc attggtatgc
     8341 ttccggctta cgtccatcac accagaccac aacttgcttt gccaggctac cgctgtccgg
     8401 gccgaacatc gggtgtaacc ccagtaccgg gccatcgtgc gccgccagca tggcctgtaa
     8461 tggtccattt ttcactgatg ccagatcaac cagaatacaa tctttcggta aaggcggtaa
     8521 tttgccgata acttgctcag taacgtggat tggcacacta acaatcacca ttccggcatc
     8581 ggcaacaata tcagccgctc gatcccagtc atgttgctcc agaatccgca cctgataacc
     8641 cgatagtgtc agcatcttct cgaacaggcg tcccatctga ccgccaccgc cgacgataac
     8701 caccggacgc agtgacggac aaagtgtttt aaatcctttg tcgttttcac tggagtaaga
     8761 ttcacgcatc acccgacgca aaacatcctc aatcagatct ggcggtacac ccagagcttc
     8821 cgcctctgcg cgccgcgagg ccaacataga tgcctcgcgc tccggaacat aaataggcag
     8881 tccaaagcgg cttttcacct cgcccacttc agcaaccagt tccagacgct tcgctaataa
     8941 attcagcagc gctttatcga cttcatcaat ttgatcgcgt aatgcggtca attcagcaac
     9001 cataataaac ctcttaagcc acgcgagccg tcagctgccc gttcagatcc tgatgaattt
     9061 cacgcagcaa ggcatcggtc atttcccagc taatgcaggc atcggttacg gatacaccgt
     9121 atttcatttc actgcgcggt tgctcggaag actgattgcc ctcgtggata ttactttcga
     9181 tcatcagacc aataattgag cgattgccat ctttgatttg agcaaccacg gattctgcca
     9241 ccgcaggctg acggcgataa tctttattgg aattaccgtg gctgcaatct accatcagag
     9301 acgggtgcag tcccgcctgt tccatctctt tttcacattg cgcaacatcc gcagggctat
     9361 agttcggcgc tttaccaccg cgcaggatga catgcccgtc cggattcccc tgagtttgta
     9421 gcaacgcaac ctgccctgcc tggttaatgc caacaaaacg gtgcggctgg gcggcggcgc
     9481 gcatagcgtt aattgctgtt gccagactgc cgtcggtgcc gtttttaaaa ccaaccggca
     9541 tggaaagccc ggaggccatt tcacggtgag tttgcgattc cgttgtacga gcaccaattg
     9601 ctgaccagct aaacagatcg cccaggtatt gcgggctatt cggatctaac gcttccgtcg
     9661 ccagtggcag tcccatattc accagctcaa gcagcaattt acgcgcgatc tgcagcccgg
     9721 cttctacatc aaaagagcca tccatatggg gatcgttaat taaccctttc cagccgacag
     9781 tggtacgggg tttttcaaaa tagacgcgca ttaccagata gaggctatcg ctgacctctg
     9841 cggcaagggc tttaaatcga cgagcatatt ccagagcagt ttccggatca tgaatggaac
     9901 aaggaccaca tactaccagc agacgaggat cgcgcccggc gataatatct gaaatggttt
     9961 tacgcgagtc agcaatctgg gcttcttgtt gcaggctcaa tggaaaagcg gccttcagtt
    10021 gttccggagt cattaaaacc tgttcgtcgg taatatgtac gttattcagc gcgtcttttt
    10081 gcatgatggc tatcctgttt atgctcgttt gcgatagttg atcctcagcg aggatgacgt
    10141 aacgataaca cataaagtaa agttttcaat ccatatttcg tacattttta tttacacagg
    10201 caatttagtc gcgctttcaa cccttacttc tgtataaata aatttacacc tcctttgaaa
    10261 acaatccgct atgctttgaa aaaggagaaa gaaatgatga aaaagtttat cgcccccttg
    10321 ttggctttac tggttagcgg atgtcagatt gatccttata ctcacgcgcc aaccttgacc
    10381 agcaccgact ggtatgatgt tggtatggaa gatgcgatat cgggcagcgc cataaaagat
    10441 gacgatgcat ttagcgattc acaggcggat cgcggtctat accttaaagg atatgccgaa
    10501 ggacaaaaga aaacttgcca gaccgatttt acttatgccc gaggactttc cggtaaaagc
    10561 tttcctgcca gctgcaacaa tgttgaaaat gccagccaac tgcatgaagt atggcaaaaa
    10621 ggggctgatg aaaatgccag cacaatacga ttaaattaat taaaacccca caaataaatt
    10681 aaacataaga ttttactcat tgtgttttat ttctcacatt gatgacgatc gcatgaaaaa
    10741 atgatatttt aatgagcatt ctgaaaaaat atcgatgcat ttcgagcgaa gatggtgggg
    10801 atccctgaat gcgtttttct caccgactgt tcttacttct gattctcctg ctgacgggag
    10861 caccaatact cgctcaggag ccttctgacg ttgcgaaaaa cgtgcgtatg atggtttccg
    10921 gtattgtcag ttacacccgc tggcctgcgc tatcagggcc accaaagtta tgtatctttt
    10981 catcttcacg ttttagtacc gccctacaag agaatgccgc gacatctttg ccttatctgc
    11041 ctgtcatcat tcacactcaa caagaggcga tgatttcagg ctgcaatggt ttttattttg
    11101 gcaacgagtc accgacattc cagatggaat taaccgaaca atatccgtca aaggcgttat
    11161 tattaattgc cgaacaaaat accgagtgca ttattggtag cgccttttgt ctgatcatcc
    11221 acaataatga cgtcagattt gccgtaaacc tggatgcctt atcgcgtagc ggtgtaaagg
    11281 tcaacccgga tgtcttaatg ctcgcacgga agaaaaatga tggataacga taattctctt
    11341 aataagcgcc ccacgtttaa aagagcatta cgcaacatca gtatgaccag catatttatc
    11401 actatgatgc tgatctggtt gctgctttcc gtgacctcgg tgctgaccct gaaacagtac
    11461 gcgcaaaaaa acctggcact gacagcagca acaatgactt acagtctgga agcagctgtc
    11521 gtttttgccg atggccctgc agcaactgaa acactggcag cgctgggcca gcaagggcaa
    11581 ttttcaactg cagaagtacg tgataagcag caaaatattc tggcgtcctg gcattacacc
    11641 cgtaaggatc caggcgatac tttcagcaat ttcataagcc actggctctt ccctgccccc
    11701 atcattcagc cgattcgtca caatggtgaa accattggcg aagtacgctt aaccgctcgc
    11761 gatagttcaa tcagccattt tatctggttt tcgctcgccg tactgaccgg ttgtattctg
    11821 ctggcatcag gcatcgcaat taccctcacc cgccatttgc acaatggcct ggtggaagca
    11881 ctgaaaaata tcaccgatgt cgtacatgat gtgcgttcca accgcaattt ttcccgacga
    11941 gtttcggaag aacgtattgc tgagtttcac cgcttcgctc tcgacttcaa cagtctgctg
    12001 gatgaaatgg aagagtggca gcttcgttta caggccaaaa atgcgcagct tctgcgtacc
    12061 gcactacatg acccattaac cgggctggct aaccgcgcag cgtttcgtag cggcatcaat
    12121 acgttgatga acaattccga tgcccgaaaa acgtcggcgt tactatttct tgatggcgat
    12181 aatttcaaat atatcaatga tacctggggt catgcgacgg gcgatagagt cttgattgaa
    12241 atcgcaaaac ggttagctga atttggcggg ctgcgacata aagcataccg cctgggcggc
    12301 gatgaattcg ctatggtgct ctatgatgta cagtcggaat ctgaagtgca gcagatatgc
    12361 tcagcactga cacaaatctt taatctcccg tttgatcttc ataatggcca tcagaccacc
    12421 atgacattaa gcattggtta cgcgatgacc attgagcacg cctctgcgga aaaattacaa
    12481 gagcttgccg atcacaatat gtatcaggcc aaacaccagc gttccgaaaa actggtgaga
    12541 taacaaggat atatcgatat gataaagcac ctggtagcac ccctgatttt cacctcacta
    12601 atactgactg gctgccagtc ccctcaggga aagtttactc ctgagcaagt cgccgctatg
    12661 caatcttatg gatttactga atccgccggc gactggtcgc tgggcttatc agatgccatt
    12721 ctgttcgcaa aaaatgacta caaattgctc ccggaaagcc agcaacagat ccaaaccatg
    12781 gcagccaaat tggcctcgac agggctaaca catgcccgta tggatggaca caccgataac
    12841 tatggtgaag acagttacaa cgaaggctta tcattgaaac gggcgaatgt cgtggccgat
    12901 gcatgggcta ttggtggaca aattccaggc agcaatctca ccacacaggg tttaggaaaa
    12961 aaatatccca tagccagtaa caagaccgcc cagggccgcg ccgagaaccg ccgcgtcgca
    13021 gtggtgatta caacccctta aaacacgagc aaacagacaa aaaaagggcc agccaattgg
    13081 ccagcccttc ttaacaggat gtcgcttaag cgaaatctta gttaagacgc tctttgatac
    13141 gagcagcctt accagtacgc tcacgcaggt agtacagttt agctttacga acagcaccac
    13201 gacgtttgac agaaatgctg tcaactaccg gagagtgagt ctggaagaca cgctcaacgc
    13261 cttcgccgtt ggaaatttta cgaacagtga atgcagagtg cagaccgcgg ttacgaatag
    13321 cgataaccac gccctcgaat gcctgcagac gttttttgga accttcaaca acccatactt
    13381 tcacttccac ggtatcaccc ggacggaagg aaggtacgtc ctgcttcatc tgctcttgtt
    13441 caagttgctt aataatgttg ctcataattt aatctcttat cctgggtaaa ctgatatctc
    13501 gggggcttac gccatcccat catgtttatg ttgctgttgt gcgtgttccg ttttgaactc
    13561 cgccagcaac cttgcttgct cttcagtcag agccaggttt tccagaagtt caggtcttct
    13621 aagccaggta cggcccagcg actgtttcaa acgccagcga cgtatctcgg catggttgcc
    13681 cgacagtaac actggcggaa cttccatccc ttctaacacc tcaggccgcg tatagtgcgg
    13741 gcaatccagc aatccttcag caaaggaatc ttccgttgcc gaggcttcat gtcccagtac
    13801 tcccggaata aaccgggaaa cggagtcaat cagcgtcatt gctggtaact caccaccact
    13861 gagaacgtaa tcgccgattg accattcttc gtcaatttcg gtttggatca cgcgctcatc
    13921 tataccttcg tagcgaccgc acaccagaat caatttttga ttcgttgcca gttcgctgac
    13981 gcccgcttga tcaagcttgc gtccctgtgg tgacagataa atcacctttg cgccttcacc
    14041 cgccgcggct tttgctgcat gaatggcgtc ccgcaagggt tgcaccatca ttaacatccc
    14101 cggtccgccg ccgtaaggac gatcgtccac ggtacggtgc cggtcatgcg tgaagtcgcg
    14161 aggactccaa ctctggatgc tcagcaggcc atttttaact gcccggccag ttaccccgta
    14221 atcggtaatt gcgcggaaca tttcaggaaa caggctaatt atgccaatcc acatagcgcc
    14281 gtcttttacc gtttatccgg tggtttaaaa accaggatcc caatctactt cgattgaacg
    14341 agtagtgaga tcgactttct tgataacctg cccatcgagg aacggtacga gacgttcctt
    14401 gataccaaac gcatctttca ggtttgcctt aatgacgaga acgtcattag atccggtttc
    14461 catcatatcg acgactttac cgagatcgta gccttcagtg gttcctacct ggcagcccat
    14521 caggtctttc cagtagtagt ccccctcttc aagctgaggc agctgcgatg aatccacgac
    14581 aatttcacaa ttcgtcagca ggttcgccgc atcacgatcg tcaacgcctt tcagcttgat
    14641 gatcatgtcc tgattgtggt gcttccagct ttccagctgg acttgctgcc actgacccgc
    14701 cttctggata aaccagggct gatagtcaaa aatgctttcg gcgtcttcgg tggaagaaaa
    14761 cactctgagc cacccacgaa taccgtaaga cgaacccatt tttcccaaaa cgatgggatc
    14821 aacaggtgct tgcgcggtga gttgtttgct catcatgacc accgtgacag attaagctgc
    14881 tttgtttact tctttgatca gcgcagcaac gcgatcagaa atagttgcgc cctggccaac
    14941 ccagtgagcg atgcgatcca gatccaggcg agtgccttct tctttttcgc tagcgattgg
    15001 gttgaagaaa ccaacgcgct cgatgaagcg accgttgcgt gcattacggc tgtcagcgac
    15061 aacaacctgg tagaacggac gctttttagc gccgtgacgt gctaaacgaa tagttaccat
    15121 aacatcctct tgtgtgaata aaacaaccgg accccatcga ggaacggagt ccggtgtcat
    15181 attaaaagcc cgaaaatttt actgatttct ggagaaaatg caatcagcag ttaataactc
    15241 tatcgcataa ggtcatttag cgaccaggaa atcccggtgg catcatccct ttcatgcttc
    15301 tcatcatctt cgccattccg cctttcttca ttttcttcat catgcgctgc atgtcgtcga
    15361 actgcttcag aaggcggttc acgtcctgca cctgcatacc gcaaccggca gcgatacgac
    15421 gcttacgcga acctttgatg atttctggct tagcgcgctc tttcatcgtc atcgagttga
    15481 tgatggcttc catacgcacc agcactttat cgtccatctg cgacttaaca ttatccggga
    15541 tctgccccat gcccggcagc ttgcccatca ggctagccat gccgcccata tttttcatct
    15601 ggcgcagttg ctcaagaaaa tcgttgagat cgaagccgtc gccttttttc agcttgctgg
    15661 ctaatttctc tgcctgcgcg cggtcaactt tgctttcgat atcttcgatc agcgacagta
    15721 cgtcgcccat gccgagaata cgcgacgcga tgcggtccgg atggaacggc tccagcgcct
    15781 cagttttctc gccaacaccg agaaacttga tcggtttgcc agtgatgtga cgaatagaga
    15841 gcgccgcacc gccgcgggca tcgccgtcca ctttggtcaa cactacgccg gtaagcggta
    15901 acgcttcatt gaatgctttt gccgtattgg ccgcatcctg accggtcatg gcgtcaacca
    15961 caaacagggt ttcaaccggg ttaatcgacg catggacttg tttgatctcg tccatcatcg
    16021 cttcgtcaac gtgcagacga ccagcggtat ccaccagcag cacgtcgtag aatttcagtt
    16081 tggcttcttt cagcgccgcg ttaacgatat ctaccggctt ctgaccaaca tcagaaggga
    16141 agaaatcaac gcccacctgc tctgccagcg tctcaagctg tttgattgcc gccgggcgat
    16201 aaacgtcggc agaaaccacc agcactttct tcttgtgctt ctcgcgcagg aacttaccga
    16261 gtttaccaac gctggttgtt ttaccggcac cttgcaggcc cgccatcagc acgaccgcag
    16321 gcggttgcgc cgccaggttc agggtctggt tctcttcgcc catcgccgca accagttcgt
    16381 tacggacgat tttgacgaac tcctgccccg gcgtcaggct cttattaact tcatgaccaa
    16441 ccgctttctc ttttacgcga ttgataaact cacgcactac cggcagagcg acatccgcct
    16501 ccagcagcgc catgcgcact tcgcgcagcg tatcttttac gttgtcttca gtgaggcgtc
    16561 cacggccact gatattgcgc agcgtgcgcg acaaacgatc ggttaaatta tcaaacattg
    16621 tctctcgcct ggggtggaaa cggttggccg caatcgcgac acatcatcag tattttgccg
    16681 cagtataaca tgaaggcgtc tttgttgtta tgcaacggtt ggagcagcgt tcacctgacg
    16741 ctatactgct tctctttctt attgctcaaa ctgtcgacat cactatgccc gtttttgctc
    16801 tgctcgcgct tgtcgcctac tccgtcagtc ttgcgctgat tgttcccggt ctgctgcaaa
    16861 aaaacggcgg ctggcggcgc atggctatta tttctgcggt cattgcgctg gtctgccacg
    16921 caatcgctct ggaagcccgc atcctgcccg acggtgatag cggacaaaac ctcagcctgc
    16981 tgaacgttgg ttcattggtc agtttgatga tctgtacggt aatgaccatt gtggcttctc
    17041 gcaatcgtgg ctggttgctg ctacccattg tctatgcctt tgcgcttatc aacctggcgc
    17101 tggcgacctt catgcccaat gaatacatca cccatctgga agcaacgcct gggatgctgg
    17161 tgcacattgg cttatcgctc ttttcctatg ccacgctaat tatcgccgcc ctgtacgcgc
    17221 tgcaactggc gtggattgat taccaactga agaacaagaa gctggcattt aaccaggaaa
    17281 tgccgccatt gatgagtatc gagcgtaaaa tgttccacat cacgcagatt ggcgtggtgc
    17341 tgctaacgct cacgctttgc actggcctgt tctacatgca caacctattt agcatggaaa
    17401 atatcgacaa ggctgtgctc tctatcgtgg cgtggtttgt ctatattgtg ctgctgtggg
    17461 gacattacca tgaaggatgg cgtggacgcc gcgtcgtctg gtttaacgtt gcgggcgcgg
    17521 taattctgac actggcctac ttcggcagcc gaattgtcca gcagttaatc agctaaaccc
    17581 agaaaaggag tttcccctgg aacacatttc tactactacg ttgatcatta ttctgatcat
    17641 catggtggtc atttcagcct atttttccgg gtccgaaacc ggaatgatga ccctcaaccg
    17701 ctatcgtctg cgacatatgg cgaaacaggg taatcgctcg gccaaacgcg tcgaaaaatt
    17761 gctgcgtaag ccagaccgcc tgataagcct ggtgttaatc ggcaataacc tggtcaatat
    17821 tcttgcctcc gcgctcggca ctattgttgg gatgcgtttg tacggcgatg cgggcgtggc
    17881 aattgcgact ggagtgctga cttttgtcgt actggtattt gctgaggtat tgccgaaaac
    17941 cattgccgcg ctgtacccgg aaaaagtcgc ttatccgagt agttttctgc tggctccgct
    18001 gcaaattttg atgatgccgc tggtctggtt gctgaatgct atcacccgta tgctgatgcg
    18061 catgatgggt atcaaaaccg atatcgtggt tagcggctct ttgagcaaag aagagttgcg
    18121 cactatcgtg cacgaatcac gctcacaaat ttcccgtcga aatcaggata tgctgctgtc
    18181 ggtgctcgat ctggaaaaaa tgaccgttga tgacatcatg gtgccgcgca gtgaaattat
    18241 cggtattgat atcaacgatg actggaaatc gattctgcgc caactctccc actcacctca
    18301 cgggcgcatc gtgctctacc gtgattcgct ggacgacgcc atcagtatgc tgcgagtacg
    18361 tgaagcctgg cggttgatgt cggagaaaaa agagttcacc aaagaaacca tgctgcgcgc
    18421 cgcggacgag atctattttg tgccggaagg tacgccgctc agcacgcagt tggtaaagtt
    18481 tcagcgcaac aaaaagaaag tcggcctggt cgtcaacgag tatggagaca ttcaggggct
    18541 ggtgacggtt gaagatattc tggaagagat tgtcggcgat ttcactacgt cgatgtcgcc
    18601 aacacttgcc gaagaggtca cgccgcaaaa cgacggttcg gtgattatcg atggcaccgc
    18661 caacgtgcgg gaaatcaaca aagcctttaa ctggcatcta ccggaagatg atgcccgcac
    18721 ggttaatggc gtcattcttg aggcactgga agagatccct gtcgcaggca cccgcgtgcg
    18781 tattggcgag tacgatatag atattctcga cgtacaggac aatatgatta agcaggtaaa
    18841 agtttttcct gtgaaaccgc tgcgcgagag tgtggcggag taacgaaaac ggccctgcat
    18901 tcgcatgcag ggccgtgaat tattacgaaa acagaaatta cgcttttgct ttcgctacag
    18961 ttaccatcgc cgcacgaatc gtacgaccat tcagcgtata acccttctgc ataatgccca
    19021 gtacgttacc tggcgcaacg tcatcagatt ccaccattgc gatagcctga tgcacattcg
    19081 ggtccagcgg cacgttagtt tcggcgatca cttcaacacc aaacttacgc acaacatcca
    19141 gcatcgactt cagcgtcagc tcaatgcctt caaccatcgc agacatatcc gggttagctt
    19201 tatcagccac ttccagcgca cgatccaggc tatcaatcac cggcagcaat tcgttgatga
    19261 atttctccag cgcgaatttg tgggcttttt gaatatccag ttcagtacgt cgacgcaggt
    19321 tttccatttc ggcttttaca cgcaaaatgc cgtcacgttc acgggtctgg gcttcagcca
    19381 gctgagcttc gagattcgca attttttcat cgcgcggatc cacctgctca gcagaagctt
    19441 ctggctcaac tgcctcaatc tcttcgtgct gatccatgat aatttcttcc ggggcttgcc
    19501 cctcaggcgt tttctgttct ttactactca tgaatttctc cgcgtttttt tcgcattcat
    19561 ctcgctaact tcgcttatta tggggatcag tttcagggtt tcaagggaag cactcacatt
    19621 gtcatcaatc ttcgcaacaa ggacctcgga aaaatgaata atcatttcaa gtgtattggc
    19681 attgtgggac acccacggca ccccactgca ctgacaacac atgaaatgct ctaccgctgg
    19741 ctgtgcacaa aaggttacga ggtcatcgtt gagcaacaaa tcgctcacga actgcaactg
    19801 aagaatgtga aaactggcac gctcgcggag attgggcaac tagctgatct cgcggtagtc
    19861 gttggtggcg acggtaatat gctgggcgcg gcacgcacac tcgcccgtta cgatattaaa
    19921 gttattggaa tcaaccgtgg caacctgggt ttcctgactg accttgaccc cgataacgcc
    19981 cagcaacagt tagccgatgt gctggaaggc cactacatca gcgagaaacg ttttttgctg
    20041 gaagcgcaag tctgtcagca agattgccag aaacgcatca gcaccgcgat aaatgaagtg
    20101 gtgcttcatc caggcaaagt ggcgcatatg attgagttcg aagtgtatat cgacgagatc
    20161 tttgcgtttt ctcagcgatc tgatggacta attatttcga cgccaacagg ctccaccgcc
    20221 tattccctct ctgcaggcgg tcctattctg accccctctc tggatgcgat taccctggtg
    20281 cccatgttcc cgcatacgtt gtcagcacga ccactggtca taaacagcag cagcacgatc
    20341 cgtctgcgtt tttcgcatcg ccgtaacgac ctggaaatca gttgcgacag ccagatagca
    20401 ctgccgattc aggaaggtga agatgtcctg attcgtcgct gtgattacca tctgaatctg
    20461 attcatccga aagattacag ttatttcaac acattaagca ccaagctcgg ctggtcaaaa
    20521 aaattattct aattttacgc cagcctcttt actgtatata aaaccagttt atactgtaca
    20581 caataacagt aatggttttt catacaggaa aacgactatg ttggcacaac tgaccatcag
    20641 caactttgct atcgttcgtg agcttgagat tgattttcat agcggcatga ccgtaataac
    20701 tggcgagacc ggcgcgggta aatctattgc aatagatgcc ctcggtcttt gtctcggtgg
    20761 tcgcgctgaa gccgacatgg tgcgtaccgg cgctgctcgc gctgacctgt gcgcccgttt
    20821 ttctctgaaa gatacgccag cggctctgcg ctggctggaa gaaaatcagc ttgaagacgg
    20881 gcatgaatgt ttgcttcgtc gcgtgatcag cagcgatggt cgctcccgtg gtttcatcaa
    20941 cggtacagct gttcctctgt cacaactgcg cgaactgggt cagttgctga ttcagatcca
    21001 tggtcagcac gctcatcaat tactcaccaa acctgagcac caaaaattcc tgcttgatgg
    21061 ctatgccaat gaaacctctc tactgcagga aatgaccgca cgttatcagt tgtggcatca
    21121 aagctgccgt gacctcgcgc atcatcaaca gttaagtcag gaacgcgccg cccgtgcgga
    21181 actgctgcaa taccaattaa aagaacttaa cgaatttaat ccgcagcccg gagagtttga
    21241 acaaatcgac gaagagtaca aacgtctggc gaacagcggt caattgctga ccaccagcca
    21301 gaatgcattg gcattaatgg ccgacggtga agacgcaaac ctgcaaagtc agctttacac
    21361 ggctaaacaa ctggtgagcg aattgattgg catggacagc aaactgtccg gcgtacttga
    21421 tatgctggaa gaagctacca tccagattgc tgaagccagc gatgaactgc gccactactg
    21481 cgatcgtctg gatctcgatc ccaaccgact atttgaactt gaacagcgca tctcaaaaca
    21541 gatttcgctg gcacgtaaac atcacgtcag ccctgaggca ttgccacagt attaccagtc
    21601 gctactggaa gaacagcagc aactggacga tcaggccgac tcacaagaaa cgcttgcgct
    21661 ggcggtaacg aaacatcatc agcaggcact ggaaatcgcg cgcgcattac accaacaacg
    21721 ccagcaatat gcagaagaac ttgcacagct gatcaccgac agtatgcatg cgctctcaat
    21781 gccgcatggg cagtttacga tcgatgttaa atttgacgag catcacctgg gcgctgacgg
    21841 tgccgatcgt attgagtttc gggtaaccac caacccaggt cagccaatgc agcctattgc
    21901 caaagtcgca tccggtggtg aattgtcccg catcgcactg gcaatccagg tcatcacggc
    21961 gcgtaaaatg gaaaccccgg cactgatttt tgatgaagtg gatgtaggga ttagcggtcc
    22021 aacagcggca gttgtcggca aactgctgcg tcaactcggc gaatcaactc aggtgatgtg
    22081 tgttacccac ctgccacaag tcgcgggatg tggtcatcaa cactattttg tcagcaaaga
    22141 aaccgatggt gcgatgacag aaacgcatat gcaatccctg aataaaaaag cgcggttaca
    22201 agagctggcg cgcctgcttg gtggcagtga agtcacacgt aatacactgg cgaatgcgaa
    22261 agaactgctt gcagcgtaaa cttttttcct gcttcacggt cagagtaaac agcaaaacgc
    22321 cgtaagaccg gaaagcaaaa ggttttaaag tgatgaaagg tctattatca tcggcatatt
    22381 acagatgagc cacgtactgc tcgggcccga aaaggaatca aatcactatg cgctgtaaaa
    22441 cgctgactgc tgcagcagca gtactattga tgttgaccgc aggctgttcc actctggagc
    22501 gagtggttta ccgtcctgac atcaaccagg ggaactatct gaccgctaac gacgtatcca
    22561 aaatacgtgt tggcatgacg caacaacaag ttgcgtacgc attgggtaca ccgctgatgt
    22621 ccgatccatt tggtacgaat acctggttct atgtcttccg ccagcaacca ggtcatgaag
    22681 gtgtaacgca gcaaacgctg acgctgacct ttaacagtag cggtgtgttg accaatattg
    22741 ataacaaacc tgcgctgagt ggtaactaat aataaagttg ctctcaaaga cgttaaaaaa
    22801 ggtgctcaat gagcaccttt tttctgtctg ttatttattc gctgattttt ctgctcgttg
    22861 cctgcgaagc tctttcggat cggcaatgag agggcgataa atctccaccc gatcgccatc
    22921 atgcacacta tcgcttagtt ttgccggacg gctgtaaatg ccgactttgt ttttagttaa
    22981 atcgatatcg gtacgcaatt ccagcaagcc actggcgcga atagcttctt caaccgtcgc
    23041 gccctcctgc agcgtcacgc gctgcaggta ctgcttctca ggtagcgcat aagccacctc
    23101 aacggcaatt ttacctggca ctgtaaacct ctttcgcacg aaccgtaaaa gcctggacca
    23161 tattagacgc cagctcttta aacacgcgac caaaggcgag ttcaatcaac ttattggtaa
    23221 actcaaagtc gagatgaaac tcgatacgac acgcctcctg gctcagcggc gtaaacttcc
    23281 atccaccaat caatttcttg aacggcccat ccaccagatt cataagaata ctttggttac
    23341 tggtcaactg gttgcgggta gtaaacgttt tgctgatccc agccttagag acatctaccg
    23401 ccgcagtcat ctgcccagga gtggactcca gaatccgact tccggtacaa cccggcaaaa
    23461 actgaggata agactgaacg tcattcacta actgatacat ttgctccgcg ctgtagggta
    23521 ccagtgcggt ccggctaatc tgaggcataa caatttccat caacaaaaat ccaacaaata
    23581 atatcattta tcctgctgta aaaaaaacgc tatcccggcg ctgggtaaca tcgggttcat
    23641 gctaagatag agccttgtcc cccgcaggat tgatatgggg tgttttcgat ttcagattac
    23701 cgatgattca cgacgcttat gacgaagaaa aaagcacata aacctggttc agcgaccatc
    23761 gcgcttaaca agcgcgcccg tcacgaatac tttatcgaag aagagttcga agcgggactt
    23821 gccctgcaag gctgggaggt taaatccctg cgcgcaggaa aagccaatat cagcgacagc
    23881 tatgtccttc tgcgtgacgg agaagcgttc ctgtttggcg caaacatcac gccaatggca
    23941 gtggcctcca cgcatgtggt gtgcgatcct agccgtaccc gcaagttact tctcaaccag
    24001 cgcgaactgg actcattgta cggtcgcgtc aatcgagaag gctataccgt agtggcgctc
    24061 tccctgtact ggaaaaatgc ctggtgcaaa gtgaaaatcg gcgtcgccaa aggcaaaaaa
    24121 cagcacgata aacgttcaga tatcaaagaa cgcgaatggc aggtggataa agcacgtatc
    24181 atgaaaaacg cccaccgtta aacctgcact ccaattattg accagttcct caccgcgcct
    24241 ccctctccgg cggcgcgaat gaacatctta ttggctatca catccgacac aaatgttgcc
    24301 atcccattgc ttaatcgaat aaaaatcagg ctacatgggt gctaaatctt taacgataac
    24361 gccattgagg ctggtcatgg cgctcataaa tctggtatac ttacctttac acattggggc
    24421 tgattctgga ttcgacggga tttgcgaaac ccaaggtgca tgccgagggg cggttggcct
    24481 cgtaaaaagc cgcaaaaaat agtcgcaaac gacgaaaact acgctttagc agcttaataa
    24541 cctgcttaga gccctctctc cctagcctcc gctcttagga cggggatcaa gagaggtcaa
    24601 acccaaaaga gatcgcgtgg aagccctgcc tggggttgaa gcgttaaaac ttaatcaggc
    24661 tagtttgtta gtggcgtgtc cgtccgcagc tggcaagcga atgtaaagac tgactaagca
    24721 tgtagtaccg aggatgtagg aatttcggac gcgggttcaa ctcccgccag ctccaccact
    24781 ttaagaagga ctacaaccgg acagtagcaa taaatacagc cacttacgga cacaagccag
    24841 acagtagtcg gactgaaaaa agacaaaaat atacacgtga aatgcacgtg cacttctaaa
    24901 aaaccccagt tatcaccgac tggggttttt caatttgtaa ctaagggtaa caaaaacacc
    24961 cccgtttttt tcatctcact caccttgata ctgtttattt gtacagttaa aaataatact
    25021 gtatacaaac acagtataga gggactttta tgcgtattga gatctgcata gccaaagaaa
    25081 aaatgactaa aatgccaaac ggtgctgtgg atgcgttaaa ggaagaatta acccgacgca
    25141 tcagtaaacg ttatgacgat gtagaagtga tcgtaaaagc caccagcaat gatgggcttt
    25201 ctgttacacg cactgcagat aaggattctg caaaaacatt tgttcaggag actctgaaag
    25261 atacctggga gtctgctgac gagtggtttg ttcactaata aacacgtaaa tcggtaacgg
    25321 ctggaaatca ttcaatacta gcactatcga aagttcacca gccaaccgca gtacgttctt
    25381 gcatacgagg tgccgcgttt tacatgtcgt accgagaatt aatttcattt tacgaaaagt
    25441 taacccgcca ttgctatctt aattttttaa aacgaagtta cctctgtcag ggtcaaaagc
    25501 acaattatct cttcctacaa tcattgaaac tgcgattgat tccctgctca aaggatgagc
    25561 cgcccttgtt ttaacaagat gtattaatgc cattttatca tacaacatac aaacatttga
    25621 attcattgaa ttcctcatga ataccccatc ttctggttca cataatgtaa cagggcatgt
    25681 tcgtaagctc tctggcactt gcaattgtgc a
//
LOCUS       DAZKHC020000064        24910 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZKHC020000064 DAZKHC020000000
VERSION     DAZKHC020000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.064"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(124..2391)
                     /gene="adiA"
                     /locus_tag="ACHOSM_RS15255"
                     /old_locus_tag="pgaptmp_003051"
     CDS             complement(124..2391)
                     /gene="adiA"
                     /locus_tag="ACHOSM_RS15255"
                     /old_locus_tag="pgaptmp_003051"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HGU4559530.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLNPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2590..3498)
                     /gene="melR"
                     /locus_tag="ACHOSM_RS15260"
                     /old_locus_tag="pgaptmp_003052"
     CDS             complement(2590..3498)
                     /gene="melR"
                     /locus_tag="ACHOSM_RS15260"
                     /old_locus_tag="pgaptmp_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HGU4559531.1"
                     /translation="MNTDTFMCSSDEKQTPSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3781..5136
                     /gene="melA"
                     /locus_tag="ACHOSM_RS15265"
                     /old_locus_tag="pgaptmp_003053"
     CDS             3781..5136
                     /gene="melA"
                     /locus_tag="ACHOSM_RS15265"
                     /old_locus_tag="pgaptmp_003053"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGU4559532.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLATYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5239..6660
                     /gene="melB"
                     /locus_tag="ACHOSM_RS15270"
                     /old_locus_tag="pgaptmp_003054"
     CDS             5239..6660
                     /gene="melB"
                     /locus_tag="ACHOSM_RS15270"
                     /old_locus_tag="pgaptmp_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HGU4559533.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGDGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6799..7428)
                     /gene="yjdF"
                     /locus_tag="ACHOSM_RS15275"
                     /old_locus_tag="pgaptmp_003055"
     CDS             complement(6799..7428)
                     /gene="yjdF"
                     /locus_tag="ACHOSM_RS15275"
                     /old_locus_tag="pgaptmp_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HGU4559534.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7551..9197)
                     /gene="fumB"
                     /locus_tag="ACHOSM_RS15280"
                     /old_locus_tag="pgaptmp_003056"
     CDS             complement(7551..9197)
                     /gene="fumB"
                     /locus_tag="ACHOSM_RS15280"
                     /old_locus_tag="pgaptmp_003056"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU4559535.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9275..10615)
                     /gene="dcuB"
                     /locus_tag="ACHOSM_RS15285"
                     /old_locus_tag="pgaptmp_003057"
     CDS             complement(9275..10615)
                     /gene="dcuB"
                     /locus_tag="ACHOSM_RS15285"
                     /old_locus_tag="pgaptmp_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HGU4559536.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11186..11905)
                     /gene="dcuR"
                     /locus_tag="ACHOSM_RS15290"
                     /old_locus_tag="pgaptmp_003058"
     CDS             complement(11186..11905)
                     /gene="dcuR"
                     /locus_tag="ACHOSM_RS15290"
                     /old_locus_tag="pgaptmp_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HGU4559537.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11902..13533)
                     /gene="dcuS"
                     /locus_tag="ACHOSM_RS15295"
                     /old_locus_tag="pgaptmp_003059"
     CDS             complement(11902..13533)
                     /gene="dcuS"
                     /locus_tag="ACHOSM_RS15295"
                     /old_locus_tag="pgaptmp_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGU4559538.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMMRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGACILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13714..13944
                     /gene="yjdI"
                     /locus_tag="ACHOSM_RS15300"
                     /old_locus_tag="pgaptmp_003060"
     CDS             13714..13944
                     /gene="yjdI"
                     /locus_tag="ACHOSM_RS15300"
                     /old_locus_tag="pgaptmp_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HGU4559539.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGELKYRHK"
     gene            13956..14227
                     /gene="yjdJ"
                     /locus_tag="ACHOSM_RS15305"
                     /old_locus_tag="pgaptmp_003061"
                     /pseudo
     CDS             13956..14227
                     /gene="yjdJ"
                     /locus_tag="ACHOSM_RS15305"
                     /old_locus_tag="pgaptmp_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            14454..14750
                     /gene="ghoS"
                     /locus_tag="ACHOSM_RS15310"
                     /old_locus_tag="pgaptmp_003062"
     CDS             14454..14750
                     /gene="ghoS"
                     /locus_tag="ACHOSM_RS15310"
                     /old_locus_tag="pgaptmp_003062"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU4559540.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            14778..14951
                     /gene="ghoT"
                     /locus_tag="ACHOSM_RS15315"
                     /old_locus_tag="pgaptmp_003063"
     CDS             14778..14951
                     /gene="ghoT"
                     /locus_tag="ACHOSM_RS15315"
                     /old_locus_tag="pgaptmp_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HGU4559541.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(15070..16587)
                     /gene="lysS"
                     /locus_tag="ACHOSM_RS15320"
                     /old_locus_tag="pgaptmp_003064"
     CDS             complement(15070..16587)
                     /gene="lysS"
                     /locus_tag="ACHOSM_RS15320"
                     /old_locus_tag="pgaptmp_003064"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU4559542.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKELAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(16824..18281)
                     /gene="dtpC"
                     /locus_tag="ACHOSM_RS15325"
                     /old_locus_tag="pgaptmp_003065"
     CDS             complement(16824..18281)
                     /gene="dtpC"
                     /locus_tag="ACHOSM_RS15325"
                     /old_locus_tag="pgaptmp_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HGU4559543.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADHLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(18340..20487)
                     /gene="cadA"
                     /locus_tag="ACHOSM_RS15330"
                     /old_locus_tag="pgaptmp_003066"
     CDS             complement(18340..20487)
                     /gene="cadA"
                     /locus_tag="ACHOSM_RS15330"
                     /old_locus_tag="pgaptmp_003066"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HGU4559544.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(20567..21901)
                     /gene="cadB"
                     /locus_tag="ACHOSM_RS15335"
                     /old_locus_tag="pgaptmp_003067"
     CDS             complement(20567..21901)
                     /gene="cadB"
                     /locus_tag="ACHOSM_RS15335"
                     /old_locus_tag="pgaptmp_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HGU4559545.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLSSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(22267..23805)
                     /gene="cadC"
                     /locus_tag="ACHOSM_RS15340"
                     /old_locus_tag="pgaptmp_003068"
     CDS             complement(22267..23805)
                     /gene="cadC"
                     /locus_tag="ACHOSM_RS15340"
                     /old_locus_tag="pgaptmp_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HGU4559546.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(24065..24179)
                     /locus_tag="ACHOSM_RS15345"
                     /old_locus_tag="pgaptmp_003069"
                     /pseudo
     CDS             complement(24065..24179)
                     /locus_tag="ACHOSM_RS15345"
                     /old_locus_tag="pgaptmp_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219006.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(24554..>24910)
                     /locus_tag="ACHOSM_RS15350"
                     /old_locus_tag="pgaptmp_003070"
     CDS             complement(24554..>24910)
                     /locus_tag="ACHOSM_RS15350"
                     /old_locus_tag="pgaptmp_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4559547.1"
                     /translation="YKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERML
                     HLFSGKPIPDNRENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELID
                     RINDIIARYFPSVLSA"
BASE COUNT         6419 a         5861 c         6120 g         6510 t
ORIGIN      
        1 ccgcgcaaaa agtatgacat ttttgtttgc agcaattttt atgaaaacgt tgccatactc
       61 aaaaggccta aggccaggcg gcgtaatgtt atttaaacaa ttacgccttc agcggaatag
      121 tggttacgct ttcacacaca taacgtggta aataccgtca ataatttcag tcccttcagt
      181 ttcgtgttca aatccaggga aatggtggtc ccaggattgc agcgagcgta aataacttac
      241 ttgcggactg tttttatcgc cgaagttttc accagacagc agcatcggga ttcctggcgg
      301 atatgggata actgagtttg ccgcgatgcg tcctggcaga ttttcaatgg ataccagttc
      361 gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg gtgatttccg ccaccggcag
      421 gccggaatag gcttcgttca accgtgcgcc agggttgttt tctttcagcc aggcaaacat
      481 ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg tcaggatatt gttcaacaag
      541 ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag tggcgtttga aggagcaaag
      601 ggtgttaacc agagtccccc atttcccacg ggttacgccc atagagaaca ggaacataat
      661 ttggaagtca gtggtgcggg taggcacaat gccgtggcga ccaagccagg cagtgaccag
      721 cgccgccgga acaccggttt cttccagttc accatcttca cccattcccg gagcaaggat
      781 gctgacttta atcgggttga gcatactcca gttatccgga atatctttga agccgtgcca
      841 gctttcgccc ggatgcatta cccagcagtc ctgaacggtg gtcagcagtt ttgttggtgc
      901 gtcagcaaag tcataggttt tgccggtttg tgggtcggtg acgacttctt tgttccacgg
      961 tttgaagaac cagctaccgt cagcggtgaa ctctttatac agccgcgcca tcgcctgacg
     1021 aaaatcaacc gcttcgtcaa tcacttcttg tgtcagtgac aggccgctgt tgccgtccat
     1081 catcgacacc gccacgtcgt tggatgcgca gatggcatac agcggggagg tggtggcatg
     1141 catcatgtag gcctggttga agcgggagaa gttaatcgcc ccacgacctt cacgtacatg
     1201 aatataagaa gcctgtgaca gcgcattcag cagtttgtgg gtggagtggg tggcgaaaac
     1261 ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca tagtgatcgg catagatcgg
     1321 gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc agacgatcgg aggttttttc
     1381 cagcagatcc tgcgcttctt tagcgttata acacacgccg tcataggtgc agttggtcac
     1441 cacgcagtaa gacggttttt gcccagcttt gtctttagtc agcgggcttt cactgatttt
     1501 cttctgcaag gtttcaggtt gcatttcctg cggatagatt ggcccgataa tgccgtagcg
     1561 gttgcggctt ggcaccatgt agactggttt cgcgcctgtc agcatcaagc cttgttcgat
     1621 ggatttatgg caattacggt caacgaccac gacatcgtta tcggtcatgc aagcctgcat
     1681 gatggtgcgg ttagagccgg aagtaccgac gactaccgac caggagcgat cggcaccaaa
     1741 tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca gtatggtcaa gcaaagaacc
     1801 gagggaagtt cgttcgatgc ccatgtcggt gcggaacaga ttttcaccat agtagtcatg
     1861 gtagaaacgt ccggcgggtg ttttggtaaa accaacgccg ccctggtggc ctggcgctgc
     1921 ccaggaatat tcatggatgt cactatattt catcagcgcg ctgaacagtg gcggcaacag
     1981 ctgctggcgg tagcgggtca tcgcggcaac ggcgcgtccg gcgataaagt cggcggtatc
     2041 ttccagaatc caggcgaatt catcgacaag ctccagcagg tcgcgatcca ttgcggcgag
     2101 ggctttttcc cgatcgccca acaggaagac cggcacgttt tgttggcgct catgaagctt
     2161 accgatcaat tgtctgacgt tttgatgttc gtccggatgt tccatttgat agctgaacat
     2221 caggcagtca atggcttcgt ttgaagagag aatggcaaaa ccatcatcaa aggaggtgga
     2281 tttaatcacg gtaacatttt gctggcttaa agcatctgcc agacgctcaa cggcgttacc
     2341 gacccaggtg tcttgatgga gaaactcgct ttcaacaatt aatactttca tcattgctta
     2401 cccggttatg aaggaatctt cgttgcgggc aagtatcttc cggccttttc gggctgtaaa
     2461 gcgcgtgaaa aacaaacatt tttgaaatat ttagttttat aaacaatacg ttatgtgatt
     2521 attttaaatt attttcactg cttattatcg acggctaaac tatttttttg gctgatactg
     2581 atatcgtctt tagccgggaa acgtctggcg gcgctgttgg ctaagtttgc ggtattgttg
     2641 cggcgacatg ccgacatatt tgccgaacgt gctgtaaaaa cgactacttg aacgaaagcc
     2701 tgccgtcagg gcaatatcga gaatactttt atcggtatcg ctcagtaacg cgcgaacgtg
     2761 gttgatgcgc atcgcggtaa tgtactgttt catcgtcaat tgcatgaccc gctggaatat
     2821 ccccattgca tagttggcgt taagtttgac gtgctcagcc acatcgttga tggtcagcgc
     2881 ctgatcatag ttttcggcaa taaagcccag catctggcta acataaaatt gcgcatggcg
     2941 cgagacgctg tttttgtgtg tgcgcgaggt tttattgacc agaatcggtt cccagccaga
     3001 gaggctaaat cgcttgagca tcaggccaat ttcatcaatg gcgagctggc gaatttgctc
     3061 gttcggactg tttaattcct gctgccagcg gcgcacttca aacgggctaa gttgctgtgt
     3121 ggccagtgat ttgatcacca tgccgtgagt gacgtggtta atcaggtctt tatccagcgg
     3181 ccaggagaga aacagatgca tcggcagatt aaaaatcgcc atgctctgac aggttccggt
     3241 atctgttagt tggtgcggtg tacaggccca gaacagcgtg atatgaccct gattgatatt
     3301 cactttttca ttgttgatca ggtattccac atcgccatcg aaaggcacat tcacttcgac
     3361 ctgaccatgc cagtggctgg tgggcatgat atgcggtgcg cgaaactcaa tctccatccg
     3421 ctggtattcc gaatacagcg acagcgggct gggggtctgt ttttcgtcgc tgctgcacat
     3481 aaacgtatct gtattcatgg atggctctct ttcctagaat atcagaatta tggcaggagt
     3541 gagggaggat gactgcgagt gggagcacgg ttttcaccct cttcccagag aggcgagggg
     3601 agcgtccgag catcatgagg ccgaaaactc tgcttttcag gtaacttatt accataaact
     3661 cagatttact gctgcttcac gcaggatctg agtttatggg aatgctcaac ctggaagccg
     3721 gaggttttct gcagattcgc ctgccatgat gaagttattc aagcaagcca ggagatctgc
     3781 atgatgactg cacccaaaat tacatttatc ggcgctggtt cgacgatttt cgttaaaaat
     3841 attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg cgcatattgc cctgatggac
     3901 attgatccca cccgcctgga agagtcgcat attgtggtgc gtaagctgat ggattcagca
     3961 ggggccagcg gcaaaatcac ctgccacacc caacagaaag aagccttaca ggatgccgat
     4021 tttgtcgtgg tggcatttca gattggcggt tatgaacctt gcacggtgac tgatttcgag
     4081 gtctgtaagc ggcatggtct ggaacaaacc attgccgata cgttggggcc gggcggtatt
     4141 atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt gcgaggacat gacggaagtc
     4201 tgccccgatg ccacattgct caactacgtt aacccaatgg cgatgaatac ctgggcgatg
     4261 tatgcccgct atccgcatat caaacaggtc gggctgtgcc attcggtgca gggaacggcg
     4321 gaagagctgg cgcgtgatct caatatcgac ccagctacgc tgcgttaccg ttgcgcaggt
     4381 atcaatcata tggcgtttta cctggagctg gagcgcaaaa ccgccgacgg cagttacgtg
     4441 aatctctacc cggaactgct ggcgacttat gaagcagggc aggcaccgaa gccgaatatt
     4501 cacggcaata ctcgctgcca gaatattgtg cgctacgaaa tgttcaaaaa gctgggctac
     4561 ttcgtcacgg aatcgtcaga acattttgcc gaatatacgc cgtggtttat taagccaggt
     4621 cgtgaggatt tgattgagcg ttataaagta ccgctggatg agtacccgaa acgctgcgtc
     4681 gagcagctgg cgaactggca taaagagctg gaggagtata aaaacgcctc ccggattgat
     4741 attaaaccgt cacgggaata tgccagcaca atcatgaacg ctatctggac tggcgagccg
     4801 agtgtgattt acggcaacgt ccgtaacgat ggtttgattg ataacctgcc acaaggatgt
     4861 tgcgtggaag tagcctgtct ggttgatgct aatggcattc agccaaccaa agtcggtacg
     4921 ctaccttcgc atctggccgc cctgatgcaa accaacatca acgtacagac gctgctgacc
     4981 gaagctattc ttacggaaaa tcgtgaccgt gtttaccacg ccgcgatgat ggacccgcat
     5041 actgccgccg tgctgggcat tgacgaaata tatgctcttg ttgacgacct gattgccgcc
     5101 cacggcgact ggctgccagg ctggttgcac cgttaaaacg cgactaaacg ctactgcgcc
     5161 gggggattta ttccggcgca cacctctgac gataccaata acagaaggcg ggcgttggta
     5221 acagcgaccc gataccctat gagcatttca atgactacaa aactcagtta tggatttgga
     5281 gcgttcggga aggattttgc gatcggcatt gtgtatatgt acctcatgta ttactacacc
     5341 gatgtcgtcg ggctgtctgt gggtttggtc ggtactttgt ttctggtggc gaggatctgg
     5401 gatgctatta acgatccgat tatgggatgg attgtaaatg ctacgcgatc gcgatggggt
     5461 aagttcaaac cctggatcct gatcggtacg ttggcaaact ctgtaatctt atttctcctc
     5521 tttagtgcgc atctgtttga aggtactact cagattgtct ttgtttgcgt gacctacatc
     5581 ctctggggca tgacttacac cattatggat attcccttct ggtcgctggt tccaaccatc
     5641 acgctcgata aacgtgagcg tgaacaactg gttccttatc cgcgtttttt tgccagtctg
     5701 gcgggctttg ttacggcagg tgtgacgcta ccatttgtta attatgtcgg cgatggcgat
     5761 cggggatttg gctttcagat gttcacactg gtactgatcg ccttttttat tgtttcaacc
     5821 atcatcactc tgcgcaatgt gcatgaagtc ttttcgtcag acaatcaacc gtctgctgaa
     5881 ggaagccatc tgacacttaa agccatcgtt gcgctaattt ataaaaacga tcagctttca
     5941 tgcctcttgg gtatggctct tgcttataat gtagccagca acattattac cggatttgct
     6001 atctattatt tctcatatgt tatcggtgat gcggatttgt tcccctatta tctgtcgtat
     6061 gcgggagctg ctaacctagt gacgttagta ttcttcccac gcttagttaa atcattatcc
     6121 cgacgcattt tatgggccgg agcatctatt cttccggtgt taagctgtgg tgttctcctg
     6181 ttaatggcat taatgagcta tcacaacgtc gtcctcattg tgattgcggg tattttgctg
     6241 aatgtgggaa cggcgctttt ctgggtacta caggtcatca tggtggcaga taccgttgat
     6301 tacggtgaat ataaactgca cgtacgctgt gaaagtatcg cttactccgt gcagactatg
     6361 gtggtgaagg gcggttcagc ctttgcggct tttttcattg cggttgtgtt agggatgatt
     6421 ggctatgtac cgaatgttga acagtctacg caagccctat taggtatgca gtttattatg
     6481 attgctctac caactctgtt tttcatggta acgctgattc tctacttccg tttctatcgc
     6541 ctcaatggcg acacgctgcg caggatccag attcatctgc tggataaata tcgcaaagta
     6601 ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag tgagtgatgt gaaagcctga
     6661 cgtggacaat agctaacgaa aatggcctga tgcgatatgt ttatcaggcc acgtcttaat
     6721 gtaatgcttt ggatttgcaa attttgtaga ccggataagg aattcacgcc gcatccggca
     6781 tcaacaaagc gcacgttgtt atccggttat caagccaaag cgccgtagct ggcggcaatg
     6841 aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc agcgcgcaga acatatcgga
     6901 ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca tccgctccct ggcccattgc
     6961 cagcgccgcc caccactcga ttaattcata catcgcgctt atcgccagcg ccacgcagca
     7021 gaccaggaac gccaccattt tacgtccgcg cacgtacatc ccgcgcacga gaatttctcg
     7081 tgccaccagt gcaggcacca gcccctggaa aaaatgcccc agcttgtcat acggattacg
     7141 gctcaacccc agccattcct gcacctcaaa accgatgggg actttcgcgt aggtgtattg
     7201 cccgccgacc atcaggatga tggcgtgaaa gaaaatgagc gtatagagca gcggcgttaa
     7261 cggataacgt ttagcggtgg caagcagtag cggcacgaca ataatcaccg gtgtcacttc
     7321 catcagccag gtgagtttgt catgggctga aatgccggta taaatcagga ttaacgttag
     7381 cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg cgggtcatgg tgagttcgca
     7441 agtcagggaa aatactgact attcaccagc aagcatgaaa ttacaatcgc aaaagaggtg
     7501 gcccaggggg atcacctggc agcatgctgc caggcgctgg gccgaagagg ttacttagtg
     7561 cagttcgcgc actgtttgtt gacgatttgc tggaagaagt cgttaccttt gtcatcgacc
     7621 aggataaacg ccgggaaatc ttctacttcg attttccaga tagcttccat acccagctcc
     7681 ggataagcga cgcattccag atgcttgatg ctctgctgcg ccagtaccgc cgccggaccg
     7741 ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg cgtcggtaac ctgctggctg
     7801 cggttgcctt tcgccagcat gatcatgctg ccgccgtggg attgcagcag atccacatag
     7861 gagtccatac gacctgcggt ggttgggcca agtgaacctg atggataacc ggcaggggtt
     7921 ttcgccggac ccgcgtagta gatcgggtga tctttgatgt actgcggaag ttctttaccg
     7981 gcgtcaatca gctctttcag cttggcgtgt gcaatatctc gacccacaat aatggtgcca
     8041 gtgagcgaca aacgagtgga aaccggatat tgcgaaagct gggcgaggat ctctttcatc
     8101 gggcggttaa ggtcgacctt caccgcttcg ccttcaccgg cctgacgcag ttcttgtgga
     8161 atgtactgac ctggattgtg ttccagtttt tcgatccaga taccttcgcg gttgattttc
     8221 gctttaatgt tacggtcagc ggagcaggag acgcccatgc ctaccgggca ggatgcgccg
     8281 tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt atttaccgcc aaactgcgcg
     8341 ccaagaccga gtttctgggc ctcttccagc agttcctgtt ccagctggac atcgcggaac
     8401 gcctgaccat gttcgttccc ttccgtcggc agttcatcgt aatagtgagc gcttgctaat
     8461 ttgacggttt tcaggttggt ttccgcagac gtaccgccaa tcacaaacgc gatatggtac
     8521 ggcgggcagg ctgcagtacc gagggtacgc attttctcga cgaggaagtt tttcagtttg
     8581 ccgggagtca gcagggcttt ggtttcctgg tagagatacg ttttgttggc agagccgccg
     8641 cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta ccgcgtacag gtcgatttgc
     8701 gcaggcaggt tagtgccggt gttgacctct ttgtacatgt ccagcgccgc gttttgcgag
     8761 tagcgcaggt tatcttcgat ataggtgtta tagacgcctt tcgacagcgc ttcttcatca
     8821 ccgccaccgg tccacacgcg ctggcctttt ttaccgacga tgatcgcggt gccggtatcc
     8881 tggcaggtcg gcagcacgcc tttggcggcg atttcggagt ttcttaagaa ttgcagcgcc
     8941 acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa tagccgcaac ctgtttctgg
     9001 tgtgccggac ggagcatgaa agaagcgtcg tgaaaggctt gctgcgccag tagggtcagg
     9061 gcttctggtt ccactttcag gatggtttcg ccgtcgaagt cggcaacgct aacgtaatcg
     9121 gaagtgagta gatagtattc ggtattgtct ttccccatcg ggaaaggtgc ctggtagata
     9181 aagggtttgt ttgacatagc ttccagcctg taactctgta tttgttattc gaaaatggcg
     9241 tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa gaacccgtac atcgcggcga
     9301 agatccagcc gaagacgcac gatacgctca caccaatcaa ccccggcaga ataaaactgt
     9361 ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg gtcaaactga atcgctgcca
     9421 gatcgctcgg ataagtcggc aggatgtaat aaccgtagca agccggtgct gaagccacga
     9481 tgtatgccgg atcaacgccg atcgccagcg cgaccggaac aatcgccgcc agcgccgcag
     9541 cctgagagtt tacaaacttg gaaaccagca gcagaacaat ggcatacgcc cacggatact
     9601 ctttcaccat ttcacccagt acgccctgaa tttcagacat atgcgcaccg aacatggttt
     9661 ctgccatcca tgcgataccg tacaccgcca cgatggcgat cataccggaa cggaagactt
     9721 cgttttttga gatagacgcg ggattggttt tggtcaggat aataatcagc gccccggtca
     9781 gcagcataaa catctgaata accagtacca tcgacagcgg tttgccgccg aaggatggac
     9841 gcaggtccga atcagcacca agaagggcga ctacagcgat tgccccgagg aaaatccaca
     9901 ttgccagcca gttgcttttc ggcagttttt tatccagcag cgtcgcggta tcaccgtaaa
     9961 catactcacg gttttccggt acggagatga atttctggaa ctcttcgtct ttatccagat
    10021 ctttaccgcg gaaccagctg aagataccga tcgccaggat accgattaac gtcgatggaa
    10081 tggtgattgc cagcagatcg aggaactcaa gatggcgacc atcaaaggtg acattaccca
    10141 gcatcgcaac cagagacacg accgcaaccg acaccggact ggcgataatc cccatctgtg
    10201 caccgataga acttgccgcc atcggacgtt ccggacggat gttgttctta atggcgacgt
    10261 cgtagatgat cggcagaatg gtgtaaacca catgacccgt accgcaaaga atggtcagcg
    10321 tacaggtcac aaacggcgcg acaattgaga catatttcgg gttgcggcgc agcaacttct
    10381 cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg caaggtcgcc gatgccgcca
    10441 ccaccgcaat gataaccagc atgacatcaa ccggtggttt acctggctga aggtggaaga
    10501 cgaagaccag aatgaccaga ccgataccgc ctaataaacc cagcgcgata ccaccctttc
    10561 tggcaccata aaacagacat atcagtatta tgataagttg gatagtaaat aacatgtgtg
    10621 aaccctcgcg ataatcctat ttaaattttt gctgaataga tcacagtcac gttctgtttt
    10681 gtatgaactg ttttcagagc ctgactaaat atccgtttgg tcgttaccgg ctttagcaaa
    10741 tacctcacag taaatattgg ctgattaaag cagcggcaaa tttatgcact gtgataagcg
    10801 gctttttcag agagaaaaac tctctggcga tgggtttata gtttgtactg cgcagatgtt
    10861 aatgttttgt ttgctgttat cttattgata tttatcggtc tattttttgt tctcagggca
    10921 gatatcatta ggtttaaaga ttaatctgat ctacccattt gtgggtaaaa atacacataa
    10981 tgcgggtgac ataatagtta attaactttt gttagcgttt tgaaattaaa aacaccgttc
    11041 acctgaagag atattaattt ttagcgatga tggagggata attatatttg atctggcaca
    11101 agttttactg atgaaggatg taacttgtgc caggggtatt ttgcattacg gtaataatta
    11161 ttacttacag ataaccagcg atccgttatt ggcaatattg tttcagtagt gagtagtgtt
    11221 ctgcctgaat acggtaacgg taaaccggac gccccgtgac gccataatgg atactggtga
    11281 acaagatgtg gcagttgacc agccagatga ggtatttacg gcaggaaaca cgcgagatgt
    11341 taacctcgtt agccagctcg tcggttgaaa actcatagtc ctgatgcgcg tcaatccact
    11401 ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg caagcgacga ggatcctgtt
    11461 cgttggagct gctgccgtgg attagctgat caagctcggc ctggtcataa tactgatgtt
    11521 tttccagcgc cattttcttt tgccgccagc cggtgagcgc ctcttcaaag cgggaagcct
    11581 ggaagggttt gatcaggtaa tccacgacac cgtaatgcag cgaatcttta atggttgccg
    11641 catcggctgc ggaggagatg acaatcacat cacttttgca acgcgcgtta tgcaggacag
    11701 gcagtaaatc gagcccgttc tctttttgca tatagatatc gagcaatatc aggtcgatag
    11761 gcgtatcgct attgaagata atctctttgg ctttctccag cgtcgaggct gttccacagc
    11821 attgaaagcc tgggatttgt gctacgtatc ggcgattcag ctccgcgacc attgcgtcgt
    11881 catcgataat taatacattg atcatctgtt cgacctctcc ccgtcccagg gtatctggac
    11941 aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg atgctgccgc cgagattttc
    12001 tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg cttccttttg tcgagacacc
    12061 tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc ggtccatcat cgttaacttc
    12121 gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta atttcgccac cgggttccgg
    12181 ccctaatgct tccagcgcgt tttctatcag atttcccaat gtggtaatca gcgtcgcgac
    12241 ctggtcctca ctgccgctgt ctggcagctg gctttcactg tttaaaatca gcgtatggcc
    12301 taaatcggtc gcgcgattaa tcttgctgat taaaaaacca gcgataaccg gagatttgat
    12361 cttacccagc agagagccaa tctcttcctg atagttattg gctgttttga gaatgtaatc
    12421 ttccaactgc ttataactct tcagatgcaa taatccgaga atcacgtgca atttattcat
    12481 aaattcgtgg gatcgttcac gaagtgcgtc ggcatagttg accagaccgt cgagtcgctg
    12541 catcagttta cgtacttcgg ttttgtccct gaaggttgaa atggcaccga tgataacgcc
    12601 attactgcgc accggaacgg tgttgatcag taatagccgg tctttaatcg taatctcttc
    12661 gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca tctaccacct gtgaccatga
    12721 gtggctgagc gtcgacagtt tctcatcgtc ctgcgactta cggtaattca gcaattcttg
    12781 tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc acggcaacga cgccttcttt
    12841 gatagactgc aacatggcct ggcgttgctc aaacagcgtg gagatttcgt agggttccag
    12901 gccgaaaagg atttttttca gtaccttaac cagaatgcag gcgccaatca gtccgaccag
    12961 catgccaaat aataccgacc agataatgct ccagcgactg tcattgattt gttgggtcac
    13021 gcggcttaac tcaaggccga tcgccaccac gccaatttgt ttatgatttt catcgtagat
    13081 gggggtaaat acgcgtaaag cctgtgccag aaaaccgcga ttgatagcga cattttcttt
    13141 gccattcagc gctttaagga tgtcatcacc tttaaatggc tgaccaatac gctgggcttc
    13201 aggatgcgag tagcgaagac tttgcatatc ggtaacgaca ataaacagca gatcgttgcg
    13261 tttgcgcacg gcttccgcga tggcctggat tccgctctcc tgcggttttt tctgcaagcc
    13321 ctgacggatt tccggcgagt cggcgagagt acgcgccact gccagtgcct tgttggccag
    13381 cccgtctcgc atcatatcac tgatttgcga gaagtaaatc agatgcacca ccaatagcac
    13441 cgagaacagt accgcgctga ccattaagat cactgtggta ctcaatttca tcggacgttt
    13501 gcgtaacatg cggtagggca atgaatgtct catcagcttc cttgtgtgac aaatttctta
    13561 agcattatct ctgatgaggc gggtaattca aagggagtaa gaatgattgg ctatataggg
    13621 gaagagactc tggcaacgga aactgccagt gctgtatgaa gattccaggg ctatgcttat
    13681 agcgataatc atactgatga gagagggaaa gtcatggatc aggcgctact ggacgagggt
    13741 tatcgctgtt ataccggcga aaagatcgat gtctatttca acactgcgat atgtcagcat
    13801 tctggcaatt gcgtacgtgg caacggcaag ttatttaatc tcaaacgaaa gccgtggatc
    13861 atgccggatg aagtcgacgt cgtcactgtg gttaaagtga ttgatacgtg cccgagcggc
    13921 gagctgaaat accgtcataa ataagcgagg gtaaaatgga aatacgcgaa ggccacaata
    13981 aattttacat taatgacgaa cagggcaagc aaatcgctga aattgtcttt gtgccgaccg
    14041 gagagaattt agcgattatc gaacataccg atgtcgatga aagcctgaaa gggcagggga
    14101 ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg tcgggaaaac gaaaaattat
    14161 cccactatgc ccatttgcga aacacgaatt tgataaaacg cgggagtatg atgatattcg
    14221 cagttgatgg gagagtacag agtcacgata tttttcatgc tctccgcgga gtgatgcagg
    14281 agagcatctg aagggtaggg ggatgcacaa agaatgggca gagagcgcgt ttttttgtcc
    14341 caagtcatcc cctttactga gcaaaaaaaa gaatatctcc tatatgagaa tcatcaatcg
    14401 gggttaataa tttttgcgtc cccagagcat tcaatattga taggagtcat attatggaag
    14461 gtaaaaacaa gttcaatact tatgttgttt cttttgatta tccatcatct tattcctcag
    14521 tgttcttaag gttaagatca ttgatgtatg atatgaattt ctcttctatc gtggctgatg
    14581 aatatgggat accacgacaa ttgaatgaaa attccttcgc aataacgaca tcgttagccg
    14641 caagtgaaat cgaagattta atcaggctta aatgcttaga cttaccggat attgattttg
    14701 acctcaacat tatgacagtt gatgactatt tccgtcagtt ttacaagtag ccaggaagga
    14761 taagaagaac ataaaaaatg gcactattct ctaaaatatt aattttttat gtgattggtg
    14821 tgaacatatc ctttgtcatt atctggttta tctcacatga gaaaacacat attcgtttac
    14881 ttagtgcatt cctggtcgga ataacctggc caatgagtct gcctgtggca ttactttttt
    14941 ctctctttta ggagctgtcg ttgagtggac ccgctggtta tcgtgaaacg aagcgtctct
    15001 cctcaaaact ggatttgcgg aacgacgaac gaagcagtcg cctgactgct tcgttcatta
    15061 aagtgaaatt tatttctgtg ggcgcatcgc cgggaagaga ataacgtcgc gaatagtatg
    15121 actgtttgta aacagcataa tcattcggtc gataccaata cccagaccag cggttggcgg
    15181 cagaccatat tccagcgcag tcacgtaatc ttcgtcatag aacatggctt cgtcgtcacc
    15241 tgcagcttta gcgttaacct gttcctggaa acgttcagcc tgatcttctg cgtcgtttaa
    15301 ttcgctaaaa ccattaccga tttcacgacc accgatgaag aattcaaaac ggtcggtgat
    15361 ttctgggtta acatcattac ggcgtgccag cggtgacact tctgccggat attccgtaat
    15421 aaaggttggc tgaatcagat gtgcttctgc cacttcatca aagatctccg tgacaatacg
    15481 tcccagcccc cagcttttct ctaccgtaat accgatagat tcagctaatt ctttagcagc
    15541 atcaaaatta tccaggtcgg ccatatcggt ttctggacga tattttttga ttgcttcgcg
    15601 catggtgagt ttttcaaacg gtttgccgaa atcaaacaca tgctcgccat aagtgacttt
    15661 agtggtaccc agaacctctt gtgccagggt gcggaacagt gactctgtca gttcaatcaa
    15721 atcgtggtaa tccgcatacg ccatgtagag ttccatcatt gtgaactcag gattatggcg
    15781 aacagaaata ccttcattac gaaagttacg gttgatttcg aatacccgtt caaaaccgcc
    15841 tacaaccaga cgtttcagat acagctccgg cgcgatacgc aggtacatat ctaaatccag
    15901 agcgttatga tgggtaataa acgggcgagc agatgcccca cctggaatta cctgcatcat
    15961 cggggtttct acttccataa agccgcgcgc gaccatgaac tggcggatag cggccagaat
    16021 ttttgaacgg acaacaaacg tttgacggga tttatcgtta gcgatgaggt ccagataacg
    16081 ttgacgataa cggacttcct gatcctgcag accatggaat ttatctggta aaggacgtag
    16141 tgcttttgtc agcaggcgca actcagtaca gtgaatggaa agctcaccgg tttgcgtctt
    16201 aaacagcgta ccgcgggcac cgataatgtc acccagatcc cattttttaa actgatcgtt
    16261 ataaacacct tctggcaggc tatctcttgc aacgtacagt tgaatacggc caccgacatc
    16321 ctgcaacgtt acaaaggagg ctttccccat gatacgacgg gtcatcattc ggccagcaac
    16381 cgagacttca atgtttaagg attccagttc ctggttattc ttcgcatcaa actcttcgtg
    16441 caactggtca gaggtatggt cgcggcgaaa atcattggga aacgccacac cttgctgacg
    16501 tagtgccgcc agtttttcgc ggcgatttct cagttcatcg ttaaaatcaa tagcctcatt
    16561 ggctccccgt gtttcttgtt cagacatttt ggttcctcta aatccagctt tcaatttagc
    16621 gtagataaag agacagatcg gtacactgta ttatctgcct cgactattag taactcagtt
    16681 tatcttatcg aaactaacgc ttacctcaag ttgatatcac taataagtaa ccatcaaaat
    16741 ggtttaaatg acttattaat ctcgatttac tagtgtgata aactgaaagt taatttattc
    16801 ataactaatt gtttttagga cagttaatcg ttgctctcct gtatcatatt cgtcggcgtg
    16861 ctaaacagaa aacgggtagc aaaggccaat acgacgatga tcgcgacaca agccaacgtc
    16921 cactctccca tctgagaaaa gaatcgctgg taagctgcaa ttgccatacc gctaatttgc
    16981 gactctgtcg tctgctgtgc cacgacgcct gccagccagt tggcgaccgc gcctgttgcc
    17041 agcatataaa taccggttaa tacgccagac attttcagac gcgtgatttg cgcaatcgcc
    17101 accgggtcaa taaagagttc ggcaaagccc attagcgcca gcccggatat catcacgccc
    17161 attgacgctt gaccgccagc cgctgcatgt cgggcatcaa atgccaacaa cataaagcca
    17221 caagccatca gcagtaagcc aaaggcaaac ttcagccaga cgcgcaatgt tgagttgccg
    17281 cggctttctg gcgacgccag ccaggccagt acaaccccag cgagcatcac cgcaattgca
    17341 ttcaccgact ggaatagtgc tgtaggtact tcaatattga atgcctgacg attcacaaag
    17401 cgatcgataa acaagctgat ggtactgccg ccctgttgtg ccagtaccca gaacaatgtc
    17461 ccgacaaaca tcaacaatac aatttgccaa agagcacggc ggtgttcggg gaatttgatc
    17521 atcatgcggg caatgatttg tgcggcaatg aggcaaacga tcgccagcaa atatccggac
    17581 cagtcgttct ccagcagcag ggtaaaaaat actggggcta aacagagcat caccaccaac
    17641 cagctccata ccggtaaggc aaatttgacg ctagtgagcg cttttttatc catactacgc
    17701 gtggactgga aatgacgatg accgcttaag aaaatcaaca aaccgataaa catgccgcca
    17761 cccgcaaggg caaagccaac atgccatcca taccactgag cagccaggcc gcaggcgatt
    17821 ggggctgcga tagaaccgat attgcccgca gcatacagca gcgaaaaacc gccatcacgt
    17881 cgatgatcgt tctcgtcgta aagctcgcca agcaaacagc tgatgtttga tttgaataaa
    17941 ccgtagccac aaataatgat tgccagcgcc agatacaggc taaaggttga atttgtatca
    18001 atgcccagca ccacatgacc aagggtcatt aacagcgcgc cggcaatcac tgcagtgcgg
    18061 ttgccgagca ggtggtcggc aagccagccg ccgagaatag gggtaacgta aaccagagaa
    18121 gcatatgcgc tgaacaggct gatggcatgg ttatcatcaa aaccaagctg atgggtgaga
    18181 tagagaatga gtaaggcacg catgccgtaa aaactgaagt actcccagat ttggatcgcc
    18241 acgatatagt atatcgcgcg cggctgtgag ggtgttttca tgtgttctcc ttatgagcaa
    18301 aaaagggaag tggcaagcca cttcccttgt acgagctaat tattttttgc tttcttcttt
    18361 caatacctta acggtatagc ggccatcagc ctgacggtat gcaccgtgaa tatcggtttc
    18421 aaagcccgga tagtgagcgc cgatttcaca cagcatctgc aggaactcca gaaccggacg
    18481 gctttcttcg gtgatcattt cacccggcat taccagagga actcccggcg gatatggaag
    18541 gatcatattg gcgttaatac gacctaccat ttcgtcgagg taaacttctt cggtcatacc
    18601 gtgcagctct ttctggaacg cagcatacgg agtcattacc atcgtcggca gcacttcaaa
    18661 tgcgcggtac atcagatccg gcagattgtg gtgaacaatc agtttgtgga tattctgagc
    18721 cagttcctga atacgcatgt tttcatagaa ttcaggatct tcacgataca gagacggcag
    18781 catgtttttc acacgcaggt tcaggtcgaa cgcacgtttg aagtcagtca gagcacgcag
    18841 caggctcagt gctttggtct tatcgatacc gatgctgaac aggaacagca ggttatacgg
    18901 accggttttc tcaacaacga tgccatgttc gtcgaggtat ttcgccacga tgctggccgg
    18961 aataccaaag tcgctcatgg tgccgtcttt ttccatcccc ggagtcagca gggtgacttt
    19021 gatcgggtca agatacatgt gctcgttatc gatgtttttg aagccgtgcc aggtgctgtc
    19081 agaacgcagc ggccagcatt cagtcgtatc gatatgatcc ggctgccata catcaaagaa
    19141 ccagccatca gattccgttc tcagacgttt gatctcttta cggaatttga tcgcacgttc
    19201 aatggaaccg ttgatcagac gtttacctgc attgcctttc atcatcgccg cagcggtttc
    19261 agtggacgcc acgataccgt agtgcggaga agtggtggtg tgcatcatgt aggcttcgtt
    19321 aaaggtttct tcgtttacgt cacctttaac gtggatcatg gaagcctgag agaacgccgc
    19381 cagcagtttg tgagtggact gggtttcgta aatcactttc ccttctacac ggccaccgct
    19441 cataccgcat ttaccttcgt aaatcggtga gaagttggtg taaggcaccc acgcggagtc
    19501 aaagtggatg gatttcacat ccagtgtttt cttgatgaag tcggtgttgt acagcagacc
    19561 atcataggta gagttggtaa ttacagcatg taccggccag gttgcgtttg gtgtttcttt
    19621 cacgcgctta gcaatggtag cgtgctggaa ttcactctgt gggataccac caagaatacc
    19681 gtaagcgtta cgggtcgggc ggaaatagat tggcgtaaca tcgctcatca tcatcaggtg
    19741 ggtcagcgat ttgtggcagt tacggtcaat cagaatggtg ctgcctgccg gagcagagta
    19801 cataccaaca attttgttcg cagtggaagt accgttggtc accatgtagc tgcggtctgc
    19861 gttaaagacg cgagcgatat actgttctgc ttctttgtgt ggaccactgt gatccagcag
    19921 agaacccagt tcagatactg aaatggaaat atcagatttc atggtattcg gaccaaagaa
    19981 atcatagaac aggctaccta ccgggctttt ctggaatgca gtaccgccca tgtgaccagg
    20041 agtacagaaa gtatatttac cttcacgaac atatttaaac agtgctttag tcagcggagg
    20101 cagaatagtg ttgatatatt cgtcagtggt ctgcttgatc ttattagcaa tatcttcagc
    20161 agcacccagc gcatattcaa agaagctaat ctgtaaacgc aggtcattca ggcttacatc
    20221 gagagtggaa tacgtattag cgaacgcgta caacggcagg ttctcgttca ttttgctaat
    20281 ttcttcgcac agctcgagat tatatttatc ccagtcaaaa ataacgccgc acagacgcgc
    20341 attgttttcg atcagtttta ataagtcgtc acggtcgttc gggtaaacaa tctggaagtt
    20401 cagacgttca agcgcgcgat gaagttcacg gatgggttct tctttaaaat aaacccccat
    20461 gtgattcaat attgcaataa cgttcatagt catatctcca ggtaaaaaag acccctccca
    20521 acacatggga caaaatgaaa ggaggagcct cggaaaatac ttttaattaa tgtgcgttag
    20581 acgcggtgtg gttatccatt gagtggctct ggcgctcgtg cattttgcga gcgtagaaca
    20641 tcaggataat caggctgacg atgaaggtac ctgccagctc gaaggagctt gcgcccatca
    20701 gcgcgatgaa gcagaacaca caacccagta cagagcagat caggctgaca aagttgcgga
    20761 tgttaacgcc ttcaaaacga atcaggtcaa cgcaagagta gaagtacggc agcatagtca
    20821 gcagtactgc gataccggtc agttcaccga acaggtcaga tgctttacca ccggcagagt
    20881 tcatcagagt gatgaggatc atcagggcag tcattttcac tgcagccagc agcagacctt
    20941 ttttcggaat accgttgctg tcgacttcac cataaacttt cgggaagtta ccgtcgttag
    21001 cggcacgtac acctgcctgg cctaccaaca tcatccagga gcccagagaa gacaggcatg
    21061 caaaggcggt gaatgcagaa accagcggcg cagcccagtt accgaggata gttgaagcac
    21121 tgattgcaaa cggagcaccg gaagccgcca ttacagaaga cggatacata ccggaaagca
    21181 cctgagtcgc agcgatataa acaatacctg ctaaaccagt acccagcatg gttgccagcg
    21241 gaacggtacg tttcgggttt ttaaccatac cagtacttac tgctgcggat tcaacaccca
    21301 cgaaggccca caggcagagc agaatacttt taatgatcgc atgaccatca gtggtatccg
    21361 cagtattcca gttagctgca taagttgccg catcaaacca atgccagcca acaatagcag
    21421 tcatcaccac aggaataaga accagcacca gaccaatagt ggttaaacgg cttacccagg
    21481 taccgccgag catatttaca aaggtaaata cccagacgat agcaatacag gcgatacccg
    21541 ccggaacagg atcatttaat actgggaaga aggtggaaag ataagataca gcggtaatac
    21601 caatcgccag gttaccaatc cagttagcat ggtaataaag aacacctgtc tgaaaaccaa
    21661 atgcagggga aatttctccg gcataagcaa ttgggccacc ttgttgcggg ttttttgttg
    21721 ccagtcgggc atatacatac gccagcgaca ttgcaccaat aatagagata atccaacccc
    21781 agatagcaat accaccgata cttgctaggt tcgcaggtaa taatgcaata ccgctcccca
    21841 tcatattacc ggcaacaaca ccggtacagg caaatagccc gatcttcttg gcagaactca
    21901 tgctcttctc ctaatttcat ttttgaattt ggagtccggg tcatgatgta taactatttc
    21961 ctgaccagac caaactggcg ataagattac tcacgaaaaa aggattaatc ctaaagatta
    22021 ggtgaaataa cacaaaagtt tctgtaagtg agaatttgag gttttttatt aacacatcag
    22081 gatcgcaagt tgatatcatg aaaagataaa cattcaatgt ttacaatgga ttgcgtggca
    22141 ttgtctggtt aggtttatgt aataaaaatt atgcggcaaa taaattgccg caacatatta
    22201 taccaacagt aacatacaaa aactcaacaa caaatattcc cgagcatgaa tcaacccggg
    22261 agttacttat tctgaagcga gaaatttgtc gagataaggt acaacataag gaacagaagt
    22321 ctggaatata ccattttcaa tccagtaaag ggtgtttgcc cctgggcgta aattaaaagc
    22381 agtgagatat gcatcagctg cttcccggtt catccccttc atttcataaa ccttgccaag
    22441 caacacataa tttagccagg acatttcaag atcaatgcca gtatttatcg cctggtaaga
    22501 ctcatctgtt ttacctttta ccagagcact gaccgctttt atttgatata taatggacag
    22561 gttgttcaat tccggcagtg taacaatgtt gtctatttct gtgttcagtg ctgctaattg
    22621 tttttcatct aaagggtgtt gagaatggcg cacgatatca actaatgctt tttctgctct
    22681 cgcgtaggta aattctgggg atgattgaac aatctcacct aataattcac tggcacgatt
    22741 caatgatttg tcatcgccat gcagtaaata atcatgagcc tgataaaaat tagttaataa
    22801 cgcaccacga tgaggcaaaa tttgctggag cgtctcctgc attcgttgtg gccacggttg
    22861 gtttaacgct tttgataaac tctccagtaa atcattttga atcgccagct gattaccgtt
    22921 agtgatgaca taacgtttat ccagcatggt tgaaccatct gcattgtcta ccaattttat
    22981 cgacataaag cattgttgag cacggtattg gcgctgatta acaaaggcaa tagataatgt
    23041 tttaccggaa ctgctcggtt catcaatgtt gtagttgatt ttgtcatgca ccataaaggt
    23101 ggagaaggtg ttaagtgatg tcgccaccaa atcacccacg cctatcgcgt aagagagctg
    23161 atacggggaa ctccagctgt tacaactttt atttaccata ttaatgtcaa tatcgcgtgg
    23221 attgagcaaa atacgcgatt tgctcatagg aagacgtgta tcaagagttg aaaacgctac
    23281 cagtgctaca cagataccta acgacaacag gaaaaaaaac catacccaaa aggtagtgaa
    23341 tcgtttgctt ttaactgggg attgttcagg tggcgttgcg gtgttttgaa tgttaagact
    23401 gtgggaggga gaatctgtgg caggaaccgc ctctggtata gggggaggcg aagatagcat
    23461 tatttcctct ccctcttctt cgctgtacca gataaccggc accattaatt tatagccgcg
    23521 ctttggtaca gtagcgatat agacaggact atcttcatca ttatctttta atgacttacg
    23581 tagttctgag atactctgcg tcacaacatg attggtgaca atacttctct tccagacatt
    23641 atcgataagt tcatccctgc taagtacttc gccactgtgt tgagcaaaga aaaccagaag
    23701 atcgattaat ctcggctcaa gggtaagttg acgcccattg cggctaattt ggtttatgga
    23761 cggagtaaca agccattcgc caacgcgaac tacaggttgt tgcataatag aaactcattc
    23821 gaaaagggaa tgatgcaatg ataattgcca caacctattt ttaccatcta tagatgggtt
    23881 tatttacata ttattggtga atgcaagacg ttatttttac cagccataaa cttcttgatt
    23941 acatagtatt acgaaaggat tttactgaga accagaagta atattcctta ccatcaaaat
    24001 tcatcatctt tgccaaagaa aaatgttcag aaaataatcc atggaaaatt gtccggagcg
    24061 cttactattt aaatggattg ttagtctttg catgagcaag cggactgata catttctctt
    24121 tgttctcatt cagaaaatct catcagtcgg cgttcagacc ggtgttgtgc tttatggatg
    24181 cgttacgctc ctgatgacgt catttgacgt tcaacaacat cacggggccg cacgacattt
    24241 cacgtcagtt agtgctatcg ctcaggaaca aattttcccg aattgggata tgcccgcaaa
    24301 ttgctggtga tgtggggaga atctggttga gttcggtaga attgatttgg aggcagaacg
    24361 cttaaattgt ggcgtccaga aacgaaaaac ggacctccgt ggaggtccgt tgatatgaat
    24421 ttggtgccgg actcggaatc gaaaatttct tttatcatat tgatattaat gcaatttgtt
    24481 tggtttgatt aaaaatgcac cctcaattat acccgcagtc ccttttgata tcgaaaggga
    24541 atcacactcg ggatcaggct gagagcacag aaggaaagta cctggctatt atatcgttaa
    24601 ttctgtcaat cagttccagc cttttgaaag tgatgtgtgc agatcccttc ttaaagtatt
    24661 taatgatgaa catctcgtct tcatagcgct ctttaccctg aacggactgg atgtgaccat
    24721 ccagacgaat agtgatgttt tcccggttgt cggggatcgg tttgccgctg aacagatgta
    24781 gcattctttc caggtcgacg agtcggtcag tctgttgccc ggtgataaga tgaaaccccc
    24841 accggtccca cctcaccagg ttgttgacga taattttact gccaaatttg caggggcaat
    24901 tggttttgta
//
LOCUS       DAZKHC020000065        24697 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZKHC020000065 DAZKHC020000000
VERSION     DAZKHC020000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.065"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            74..226
                     /gene="hokA"
                     /locus_tag="ACHOSM_RS15355"
                     /old_locus_tag="pgaptmp_003071"
     CDS             74..226
                     /gene="hokA"
                     /locus_tag="ACHOSM_RS15355"
                     /old_locus_tag="pgaptmp_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HGU4559548.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(215..274)
                     /locus_tag="ACHOSM_RS15360"
                     /old_locus_tag="pgaptmp_003072"
     CDS             complement(215..274)
                     /locus_tag="ACHOSM_RS15360"
                     /old_locus_tag="pgaptmp_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559549.1"
                     /translation="MRNAVRKAGITSRRRLLLF"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHOSM_RS15365"
                     /old_locus_tag="pgaptmp_003073"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="ACHOSM_RS15365"
                     /old_locus_tag="pgaptmp_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HGU4559550.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /gene="yiaG"
                     /locus_tag="ACHOSM_RS15370"
                     /old_locus_tag="pgaptmp_003074"
     CDS             complement(907..1197)
                     /gene="yiaG"
                     /locus_tag="ACHOSM_RS15370"
                     /old_locus_tag="pgaptmp_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HGU4559551.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1631..2341
                     /gene="yiaF"
                     /locus_tag="ACHOSM_RS15375"
                     /old_locus_tag="pgaptmp_003075"
     CDS             1631..2341
                     /gene="yiaF"
                     /locus_tag="ACHOSM_RS15375"
                     /old_locus_tag="pgaptmp_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HGU4559552.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHOSM_RS15380"
                     /old_locus_tag="pgaptmp_003076"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="ACHOSM_RS15380"
                     /old_locus_tag="pgaptmp_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HGU4559553.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4128)
                     /gene="yiaD"
                     /locus_tag="ACHOSM_RS15385"
                     /old_locus_tag="pgaptmp_003077"
     CDS             complement(3469..4128)
                     /gene="yiaD"
                     /locus_tag="ACHOSM_RS15385"
                     /old_locus_tag="pgaptmp_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HGU4559554.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4281..6614
                     /gene="bisC"
                     /locus_tag="ACHOSM_RS15390"
                     /old_locus_tag="pgaptmp_003078"
     CDS             4281..6614
                     /gene="bisC"
                     /locus_tag="ACHOSM_RS15390"
                     /old_locus_tag="pgaptmp_003078"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HGU4559555.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAGICGVHAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGSDAVDK
                     IPVARIVEALENPGCAYQHNGMNRLFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6583..7023)
                     /gene="yiaC"
                     /locus_tag="ACHOSM_RS15395"
                     /old_locus_tag="pgaptmp_003079"
     CDS             complement(6583..7023)
                     /gene="yiaC"
                     /locus_tag="ACHOSM_RS15395"
                     /old_locus_tag="pgaptmp_003079"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU4559556.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7020..7583)
                     /gene="tag"
                     /locus_tag="ACHOSM_RS15400"
                     /old_locus_tag="pgaptmp_003080"
     CDS             complement(7020..7583)
                     /gene="tag"
                     /locus_tag="ACHOSM_RS15400"
                     /old_locus_tag="pgaptmp_003080"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGU4559557.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7741..8439
                     /gene="yhjY"
                     /locus_tag="ACHOSM_RS15405"
                     /old_locus_tag="pgaptmp_003081"
     CDS             7741..8439
                     /gene="yhjY"
                     /locus_tag="ACHOSM_RS15405"
                     /old_locus_tag="pgaptmp_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGU4559558.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSAKF"
     gene            8668..9876
                     /gene="yhjX"
                     /locus_tag="ACHOSM_RS15410"
                     /old_locus_tag="pgaptmp_003082"
     CDS             8668..9876
                     /gene="yhjX"
                     /locus_tag="ACHOSM_RS15410"
                     /old_locus_tag="pgaptmp_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4559559.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10200..11891
                     /gene="eptB"
                     /locus_tag="ACHOSM_RS15415"
                     /old_locus_tag="pgaptmp_003083"
     CDS             10200..11891
                     /gene="eptB"
                     /locus_tag="ACHOSM_RS15415"
                     /old_locus_tag="pgaptmp_003083"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HGU4559560.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            11983..12059
                     /locus_tag="ACHOSM_RS15420"
                     /old_locus_tag="pgaptmp_003084"
     tRNA            11983..12059
                     /locus_tag="ACHOSM_RS15420"
                     /old_locus_tag="pgaptmp_003084"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12017..12019,aa:Pro,seq:cgg)
     gene            12094..12227
                     /locus_tag="ACHOSM_RS15425"
                     /old_locus_tag="pgaptmp_003085"
     ncRNA           12094..12227
                     /ncRNA_class="other"
                     /locus_tag="ACHOSM_RS15425"
                     /old_locus_tag="pgaptmp_003085"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            12972..14579
                     /gene="dppA"
                     /locus_tag="ACHOSM_RS15430"
                     /old_locus_tag="pgaptmp_003086"
     CDS             12972..14579
                     /gene="dppA"
                     /locus_tag="ACHOSM_RS15430"
                     /old_locus_tag="pgaptmp_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HGU4559561.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14887..15906
                     /gene="dppB"
                     /locus_tag="ACHOSM_RS15435"
                     /old_locus_tag="pgaptmp_003087"
     CDS             14887..15906
                     /gene="dppB"
                     /locus_tag="ACHOSM_RS15435"
                     /old_locus_tag="pgaptmp_003087"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HGU4559562.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15916..16818
                     /gene="dppC"
                     /locus_tag="ACHOSM_RS15440"
                     /old_locus_tag="pgaptmp_003088"
     CDS             15916..16818
                     /gene="dppC"
                     /locus_tag="ACHOSM_RS15440"
                     /old_locus_tag="pgaptmp_003088"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HGU4559563.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16829..17812
                     /gene="dppD"
                     /locus_tag="ACHOSM_RS15445"
                     /old_locus_tag="pgaptmp_003089"
     CDS             16829..17812
                     /gene="dppD"
                     /locus_tag="ACHOSM_RS15445"
                     /old_locus_tag="pgaptmp_003089"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HGU4559564.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17809..18813
                     /gene="dppF"
                     /locus_tag="ACHOSM_RS15450"
                     /old_locus_tag="pgaptmp_003090"
     CDS             17809..18813
                     /gene="dppF"
                     /locus_tag="ACHOSM_RS15450"
                     /old_locus_tag="pgaptmp_003090"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HGU4559565.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCAQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18843..20114)
                     /gene="yhjV"
                     /locus_tag="ACHOSM_RS15455"
                     /old_locus_tag="pgaptmp_003091"
     CDS             complement(18843..20114)
                     /gene="yhjV"
                     /locus_tag="ACHOSM_RS15455"
                     /old_locus_tag="pgaptmp_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HGU4559566.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20590..20697
                     /gene="ldrD"
                     /locus_tag="ACHOSM_RS15460"
                     /old_locus_tag="pgaptmp_003092"
     CDS             20590..20697
                     /gene="ldrD"
                     /locus_tag="ACHOSM_RS15460"
                     /old_locus_tag="pgaptmp_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4559567.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(20784..22463)
                     /gene="bcsG"
                     /locus_tag="ACHOSM_RS15465"
                     /old_locus_tag="pgaptmp_003093"
     CDS             complement(20784..22463)
                     /gene="bcsG"
                     /locus_tag="ACHOSM_RS15465"
                     /old_locus_tag="pgaptmp_003093"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HGU4559568.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(22460..22651)
                     /gene="bcsF"
                     /locus_tag="ACHOSM_RS15470"
                     /old_locus_tag="pgaptmp_003094"
     CDS             complement(22460..22651)
                     /gene="bcsF"
                     /locus_tag="ACHOSM_RS15470"
                     /old_locus_tag="pgaptmp_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HGU4559569.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(22648..24219)
                     /gene="bcsE"
                     /locus_tag="ACHOSM_RS15475"
                     /old_locus_tag="pgaptmp_003095"
     CDS             complement(22648..24219)
                     /gene="bcsE"
                     /locus_tag="ACHOSM_RS15475"
                     /old_locus_tag="pgaptmp_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HGU4559570.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDYQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            24492..24680
                     /gene="bcsR"
                     /locus_tag="ACHOSM_RS15480"
                     /old_locus_tag="pgaptmp_003096"
     CDS             24492..24680
                     /gene="bcsR"
                     /locus_tag="ACHOSM_RS15480"
                     /old_locus_tag="pgaptmp_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HGU4559571.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         5997 a         6127 c         6461 g         6112 t
ORIGIN      
        1 gtgaggctac cctatgcctg aacaccataa ggttagcctc ttactcgttg gaaatcaaca
       61 cagggggctg ggaatgccgc agaaatatag attactttct ttaatagtga tttgtttcac
      121 gcttttattt ttcacctgga tgataagaga ttcactgtgt gaattgcata ttaaacaggg
      181 gagttatgag ctggcggcat ttttagcctg caatttaaaa gagtaagagt cttcggcggg
      241 aagttattcc cgcctttctt acggcgttgc gcattctcat tgcacccaaa tttatttttc
      301 acaaaaataa tactagattt tattacgcga tcgattattt atttcctgaa aaacaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacacg
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cacgcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat tccatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaac cctgtccctt ttcgtaactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgaactgata aatgccgata tatctcttac
     1381 cactcctgtt ttgtaaagat ttcgaaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattctgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ttggcacaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcgcacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttcggcaaaa
     3241 attgctgcat tttgttcgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgctcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc agggtcgcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg ttaatcctta
     4141 ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc gtgaacatga
     4201 agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc aatacatcta
     4261 acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt ctgactgccg
     4321 cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc tcgcgtggcg
     4381 cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag gttcacagca
     4441 atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg gaaaacccgc
     4501 aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg ctggatctta
     4561 ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt tttgctggtt
     4621 cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta caacgctata
     4681 tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggc gcggcacagg
     4741 cgatcatgcc gtatgtcgtg ggtggtagcg aagtttatca acagcagacc agttggccgc
     4801 tggtgctgga acatagcgat gtcgtggtgc tgtggagtgc taatccactc aatacgctga
     4861 aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca ctgcgtgaca
     4921 gcgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat ttctttggcg
     4981 ataaaatgga gtgggtggca ccgcacatgg gtaccgatgt tgcgctgatg ctggggatcg
     5041 cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcgt tgcaccacag
     5101 gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcc aaaaccgccg
     5161 aatgggcggc agggatttgt ggggttcacg cagcgaaaat tcgcgagttg gcggctcttt
     5221 tccaccaaaa cacgaccatg ctgatggctg gctggggaat gcagcgccaa cagtttggtg
     5281 agcaaaaaca ctggatgatc gtcacgctgg cggcaatgtt ggggcaaatc ggcacacccg
     5341 gcggcggttt tggtctttct tatcattttg ccaatggtgg taaccccacg cggcgttctg
     5401 cggtgctctc ttccatgcag ggtagcttgc cgggtggcag cgatgcggta gataaaatcc
     5461 ctgttgcccg tattgttgaa gcactggaaa accctggctg cgcataccaa cacaatggca
     5521 tgaatcgcct cttcccggat attcgcttta tctggtgggc gggtggcgcc aacttcaccc
     5581 atcatcagga cactaatcgc ctgatacgag cttggcaaaa accggagctg gtagttatct
     5641 ccgaatgctt ctggacggcg gcggcaaaac acgcggatat cgttctgcct gcgactacct
     5701 cttttgagcg taatgatctc accatgaccg gcgattacag taatcagcat ctggtgccga
     5761 tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt tttgccgagt
     5821 taagtgaacg ctgggagaag ggcggttatg cacgttttac ggaaggaaaa agtgagctgc
     5881 aatggctgga aacgttttat aacgttgccc gacagcgcgg ggcaagccag caggttgaat
     5941 tgccgccatt tgctgagttc tggcaagcca atcagttaat tgagatgccg gaaaacccgg
     6001 acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgcaggcg catccattaa
     6061 aaaccgccag cggtaagatt gaaatcttct cgcagcgtat tgccgattac gcttatccgg
     6121 attgcccggg gcatccaatg tggctggagc cggacgaatg gcagggcaat gccgaaccag
     6181 aacagttgca ggtactttct gcccatccgg cgcaccgcct gcacagtcag ctgaattaca
     6241 gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt catcctgacg
     6301 atgctcaggc gcgcggcata accgaagggg atatggtgcg ggtgtggaac tcccgtgggc
     6361 agatccttgc cggagcggtc attagcgagg gaattaaacc tggcgtgatt tgcattcacg
     6421 aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac ggcgcagtga
     6481 acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg ggtaatacgg
     6541 cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg tttgaaccac
     6601 cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc aatcgacaat
     6661 gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga tttttttgat aaacctccag
     6721 catcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa taccgcgtct
     6781 gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa tgctgacaaa
     6841 accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa gataggcatc
     6901 ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat gcccccaggt
     6961 tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg cttcccgaat
     7021 catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac cagcccacat
     7081 gcctgcataa aggagtaaca gattgtggtg ccgacaaact taaaaccacg ttttttcagt
     7141 gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa cgttgtggct
     7201 tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcggcaaa cggttcgccg
     7261 ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat tttccctcga
     7321 tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg cattgcggca
     7381 accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt tttgaggacg
     7441 gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa cagtttttta
     7501 ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag cgggtcctga
     7561 ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt aaaaatttca
     7621 gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct actatgcccg
     7681 atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg agtcgtgtta
     7741 atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag cgtggtaatc
     7801 agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatattgt tgatacgcaa
     7861 cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga ttacaacgat
     7921 attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt caatctggcg
     7981 gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt tcctttatat
     8041 gaacaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac cacctcgatg
     8101 tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca tgccagcgtg
     8161 agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg ggcgcaaatc
     8221 agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct gagccggatg
     8281 acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga tatgttgctc
     8341 aatcaaaata ttgccgccta tgctgcgcta actcaggcag aaaataccac taataatagc
     8401 gactatctgt atacgatggg ggttagcgcc aaattttaac gtaacagtca caattgaaac
     8461 cagtgaataa caatagttgt ggcgatagtg ggtgctaact taccaaataa taaatttggt
     8521 gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc tgatggcatt
     8581 tcatgccgtt tttccccagg cataaaatgc acttcgttat ggttgtcggc agagattttt
     8641 cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg tacccgctgg
     8701 ctgacactca tcggtactat cattacccag tttgcgctgg ggtcggttta tacctggagc
     8761 ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt cgctttctct
     8821 ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa gttgcaggaa
     8881 cgttttggcg ttaaacgcgt caccatggct tccggcattt tgctgggatt aggcttcttc
     8941 ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt gctggtcggt
     9001 ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg gttcccggag
     9061 cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtctgggtag cctgggtttc
     9121 aaatttatcg acacgcagct gctcgaaacg gtgggtctgg aaaaaacctt tgtgatttgg
     9181 ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga cgcaccaaaa
     9241 caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc agagtcgatg
     9301 cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat gagcggcctg
     9361 tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga tgtggtttcc
     9421 gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct ggtgctgggc
     9481 attctgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt gatttcgctg
     9541 gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt tgcagcgatt
     9601 gcctgcgtgg catttaactt tggcggcact attaccgtct ttccatcact ggtcagtgag
     9661 ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg tttcggtatc
     9721 ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt gaccttctac
     9781 gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacgattcg tcagccagag
     9841 cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct cagccgcgag ctactgggct
     9901 tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata aacaatgact cgtaaaattt
     9961 gggccagtta actggccctt tttactgctt tttgtaacgc taaagtctct tttcaaactt
    10021 gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc acactttttc gctttgctgt
    10081 ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac ttttcccctg
    10141 ccgggcctga aaagccgcta agcagggtgt tatcacctgt ttgtccaggg tttgtttgca
    10201 tgagatacat caaatcgatt acacagcaga agctgagctt tttgcttgca atctatattg
    10261 gcctttttat gaatggcgcg gttttttatc gccgcttcgg cagttatgcg cacgatttta
    10321 ccgtctggaa aggcgtttct gctgttgttg aactggccgc caccgtactg gtgaccttct
    10381 ttttactacg tcttctatcg ctgtttggcc gccgcagctg gcgtattctg gcgtcgctgg
    10441 tggtgctctt ttccgcaggt gccagctatt acatgacctt ccttaatgtg gtcattggtt
    10501 atggcatcat cgcttccgtc atgaccaccg atatcgacct gtcaaaagaa gttgttggtc
    10561 tgaactttat tctctggtta atcgccgtta gtgcattgcc tcttatcctt atctggaata
    10621 accgctgtcg ctacaccttg ctccgacaac tgcgaacccc agggcagcgt attcgcagcc
    10681 tggcggtcgt cgtactggcg ggtattatgg tttgggcacc gattcgtttg ctggatatcc
    10741 agcagaagaa agtggagagg gcgaccggcg ttgatttgcc gagttatggc ggtgtcgtag
    10801 cgaactctta tctgccatca aactggcttt ctgcgttggg gctgtatgcc tgggcgcggg
    10861 tcgatgaatc ttccgataat aattcattgc ttaatccggc gaagaaattc acttatcagg
    10921 caccgcaaaa cgttgatgac acttatgtcg tgtttatcat cggtgaaacc acgcgttggg
    10981 accatatggg tattttcggc tatgagcgta ataccacgcc gaaactggcc caggagaaaa
    11041 atctggcggc gttccgtggt tactcctgtg ataccgcaac caaactctca ctgcgttgca
    11101 tgtttgtacg tcaggggggc gcggaagata atccgcagcg cacattaaaa gaacagaaca
    11161 ttttcgcggt tctgaagcag ttaggattca gttctgacct ctacgctatg cagagcgaaa
    11221 tgtggttcta cagcaacacg atggcggaca acattgctta tcgtgagcag attggtgcgg
    11281 agccacgtaa tcgtggcaag ccggtagatg atatgttgct ggtagacgaa atgcagcaat
    11341 cgctagggcg caacccggat ggtaagcatc tgatcattct gcataccaaa ggttcgcatt
    11401 ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg gaagccggaa tgtattggtg
    11461 ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta tgacaactcg gtgacctatg
    11521 tggatcactt tatctccagc gtgattgatc aggttcgcga taagaaagcg attgtgttct
    11581 acgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc acgccgcgtg
    11641 aactggcacc gccggagcag ttccgcgtac cgatgatggt ctggatgtca gataaatatc
    11701 tggaaaatcc ggtcaatgcg caggcgtttg cgcagctgaa aaaagaagcc gacatgaaag
    11761 tgccacgccg tcacgtagag ctgtacgaca ccatcatggg ttgtcttggc tatacttcac
    11821 cggatggtgg aattaacgaa aacaacaact ggtgtcacat cccgcagaca aaagaggcag
    11881 cggctaacta agaggcctgc tgactttctc gccgatcaaa aggcattttg ctattaaggg
    11941 attgacgagg gcgtatctgc gcagtaagat gcgcctcgca ttcggtgatt ggcgcagcct
    12001 ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat caccgaccaa
    12061 attcgaaaag cctgctcaac gagcaggctt ttttgcatct gcagctcatg aggatgagaa
    12121 cctccggggg cagggaggtt cgactcgagc gaagcgagag aacgttgcgc cagcaacggc
    12181 ccgcagggcg agccacgaag tggcgagtaa tcctctatca ccgaccaaat tcgaaaagcc
    12241 tgctcaacga gcaggctttt ttgcatttgt agctcatgag gatgagaacc tccgggggca
    12301 gggaggttcg actcgacgta ggcctgataa gacgcgccag cgtcgcatca ggcaacggca
    12361 ccgcacttat tgtcggatgc ggcacgaccg ccttattcga cctacacacc acacatttcc
    12421 ctgccaaatt tgcgccccat atctcactat ccattaacgg atttgtgaca gaaaccattg
    12481 tcttttttta tatttgctta aatctttttt tgcgttactt atggctgacc ctggcatttt
    12541 ccgctgtgcg ttttagcgtt catggtatta atcgctcaga tatccacctt ttcctcagat
    12601 ttttttcatt aaatgcataa aacttaatca ccaaatgttg ctaaatatta agctgattgt
    12661 tctggtcatg ttggagtagc acaaatttct ctgctgcaaa tagctgtttg aagttttttt
    12721 aatctttgtt tgtggattct caccgttggt gtaaatcccg gttggctgta ttgacgtttt
    12781 cacattctgt tgacagattg taggtcacga ggggcatttt atggaggatc cgcactgtta
    12841 cactgatgtt aattagtacg gcatccccac ctcataacgt tgacccgacc gggcaaaaaa
    12901 caaaaaaggt caggcagcga caacccactg caaagggtta aaacaacaaa catcacaatt
    12961 ggagcagaat aatgcgtatt tccttgaaaa agtcagggat gctgaagctt ggtctcagcc
    13021 tggtggctat gaccgtcgca gcaagtgttc aggctaaaac tctggtttat tgctcagaag
    13081 gatctccgga agggtttaac ccgcagctgt ttacctccgg caccacctat gacgcctctt
    13141 ccgtaccgct ttataaccgc ctggttgaat ttaaaatcgg caccactgaa gtgatcccgg
    13201 gcctcgctga aaagtgggaa gtcagcgaag acggtaaaac ctataccttc catctgcgta
    13261 aaggtgtgaa gtggcacgac aataaagaat tcaaaccgac gcgtgaactg aacgccgatg
    13321 acgtggtgtt ctcgttcgac cgtcagaaaa acgcgcaaaa cccgtaccat aaagtctctg
    13381 gcggcagcta cgaatacttc gaaggcatgg gcttgccgga gctgatcagt gaagtgaaaa
    13441 aggtggacga caacacagtt cagttcgtac tgactcgccc ggaagcgccg ttcctcgctg
    13501 atctggcaat ggacttcgcc tctattctgt caaaagaata tgctgacgcg atgatgaaag
    13561 ccggtacacc ggaaaaactg gacctcaacc caatcggaac cggtccgttc cagttacagc
    13621 agtaccaaaa agattcccgt atccgctaca aagcgtttga tggctactgg ggcaccaaac
    13681 cgcagatcga tacgctggtt ttctctatta cccctgatgc ttccgtgcgt tacgcgaaat
    13741 tgcagaagaa tgaatgccag gtgatgccgt acccgaaccc ggcagacatc gctcgcatga
    13801 agcaggataa atccatcaac ctgatggaaa tgccggggct gaacgtcggc tacctctcct
    13861 ataacgtgca gaaaaaaccg ctggatgacg tgaaagttcg ccaggcactg acctacgcgg
    13921 tgaacaaaga cgcgatcatc aaagcggttt atcagggcgc gggtgtatca gcgaaaaacc
    13981 tgatcccgcc aaccatgtgg ggctataacg acgacgttca ggattacacc tacgatcctg
    14041 aaaaagcgaa agccttgctg aaagaagcgg gtctggaaaa aggtttctcc atcgacctgt
    14101 gggcgatgcc ggtacaacgt ccgtataacc cgaacgctcg ccgcatggcg gagatgattc
    14161 aggcagactg ggcgaaagtg ggcgtgcagg ccaaaatcgt cacctacgaa tggggtgagt
    14221 acctcaagcg tgcgaaagat ggcgaacatc agacggtaat gatgggctgg actggcgata
    14281 acggggaccc tgataacttc ttcgccaccc tgttcagctg cgccgcctct gaacaaggtt
    14341 ctaactactc aaaatggtgc tacaaaccgt ttgaagatct gattcaaccg gcgcgtgcta
    14401 ccgacgacca taacaaacgc gttgaactgt acaagcaagc gcaggtggtg atgcacgatc
    14461 agactccggc actgatcatc gctcactcca ccgtgtttga accggtacgc aaagaagtca
    14521 aaggctatgt ggttgatcca ttaggcaaac atcacttcga aaacgtctct atcgaataat
    14581 taaaagccat acaagactga tggcaaaggc aaaactgcct gatgtgctcc gcttatcagg
    14641 cctacgaaaa ttctgcaatg tattgaattt gcacgatttt gtaggccgga taaggcgtta
    14701 acgccgcatc cggcataaac aaagcgcact ttgtcaacaa tctgtatacc cggtggcgct
    14761 gtgcctctgc gtgcagcgcc atccggcagc aatacttcat tccctgtccg ataagggcgg
    14821 gaattgattt gtgagcaata cagacacgca gttccaggct gcgggtcact atagagaatc
    14881 cgggttatgt tgcagtttat tctccgacgt ttgggactcg tcatccccac gtttatcggt
    14941 attacccttc tcacatttgc ctttgtccac atgatcccgg gcgatccggt gatgatcatg
    15001 gcaggcgaac gtgggatctc ccctgagcgt cacgcgcagc tgctggctga actcggctta
    15061 gataaaccga tgtggcagca gtatctccat tacatttggg gcgtaatgca cggcgatcta
    15121 ggcatttcaa tgaaaagccg aattccagta tgggaagagt tcgtgccgcg cctccaggcc
    15181 acgctggaac ttggcgtctg cgcgatgatt tttgcgactg cggtcggtat tccggtcggc
    15241 gtgctggctg cggttaaacg tggttccatt ttcgatcaca cagcggttgg cctggcgctg
    15301 accggctact cgatgccgat cttctggtgg ggcatgatgc tgatcatgct ggtttcggtg
    15361 cactggaacc tgacgcccgt ctccggtcgc gtgagcgata tggtgttcct cgatgactcc
    15421 aatccgttaa ccggttttat gctaatcgac accgccatct ggggtgaaga cggcaacttt
    15481 atcgatgccg tcgcccatat gatcttaccg gccattgtgc tgggaactat tccgctggcg
    15541 gtcattgtgc gtatgacacg ctcctcgatg ctggaagtgc tgggtgaaga ttacatccgc
    15601 accgcgcgcg ccaaagggtt gacccgcatg cgggtgatta tcgtccatgc gctgcgtaac
    15661 gcgatgctgc cggtagtgac cgttatcggc ctgcaggtgg gaacattgct ggcgggggcg
    15721 attctgaccg aaaccatttt ctcgtggccc ggtctggggc gctggttgat tgacgcactg
    15781 caacgccgcg attatccggt ggtgcagggc ggcgtattgc tggtggcgac gatgattatc
    15841 ctcgtcaacc tgctggtcga tctgctgtac ggcgtggtga acccacgtat tcgtcataag
    15901 aagtaagggg acatcatgtc acaggttact gaaaataaag tgattagcgc accggtgccg
    15961 atgaccccgt tacaggagtt ctggcactat tttaaacgca ataaaggcgc ggttgtcggg
    16021 ctggtttatg tcgtcatcgt gctgtttatc gcgatctttg ccaactggat tgcaccctat
    16081 aacccggcgg aacagttccg cgatgcactg ctcgccccgc cagcctggca ggaaggcggc
    16141 agcatggcgc acttgttggg caccgatgac gtaggccgtg atgtgctgtc gcggctgatg
    16201 tacggcgcgc gcttgtcgct gctggttggc tgtctggtag ttgtgttatc gctgattatg
    16261 ggcgttattc tcggcctgat cgccggttac tttggcggcc tggtcgataa catcattatg
    16321 cgcgtggtcg atatcatgct ggcgctgcca agtctgctgc tggcgctggt gctggtggca
    16381 attttcggcc cgtcgattgg taacgccgcg ctggcactga ccttcgttgc cttgccgcac
    16441 tatgtgcgct taacccgcgc cgccgtgctg gtggaagtta accgcgatta cgtcaccgcg
    16501 tctcgcgtgg cgggtgccgg ggcgatgcgt cagatgttta ttaacatctt ccctaactgc
    16561 ctggcaccgc tgattgttca ggcgtcgctc ggtttctcta acgccattct cgatatggct
    16621 gctcttggtt tcctcggcat gggggcacag ccgccaacgc cggagtgggg caccatgctc
    16681 tccgacgtgt tgcagttcgc gcaaagcgcc tggtgggtcg tgaccttccc gggtctggcg
    16741 atcctgctga cggtgctggc atttaacctg atgggtgacg gtctgcgtga cgcgctcgat
    16801 cccaaactga agcagtaaga ggttcgagat ggcgttatta aatgtagata aattatcggt
    16861 gcatttcggc gacgaaagcg cgccgttccg cgccgtagac cgtatcagct acagcgtaaa
    16921 acagggcgaa gtggtcggga ttgtgggtga gtccggctct ggtaaatcgg tcagttcgct
    16981 ggcgattatg gggctgattg attatccggg ccgcgtaatg gcagaaaaac tggagtttaa
    17041 cggccaggat ttgcagcgta tctcagaaaa agagcgccgc aacctggtgg gtgccgaagt
    17101 ggcgatgatc ttccaggacc cgatgaccag ccttaacccg tgctacaccg tgggtttcca
    17161 gattatggaa gcgattaagg tgcatcaggg cggcaacaaa agtacccgcc gtcagcgagc
    17221 gattgacctg ctgaatcagg tcggtattcc cgatccggca tcgcgtctgg atgtttaccc
    17281 gcatcagctt tccggcggca tgagccagcg cgtgatgatc gccatggcga ttgcctgtcg
    17341 gccaaaactg ctgattgccg atgaaccgac caccgcgctg gacgtgacca ttcaggcgca
    17401 aatcatcgaa ctactgctgg agctacagca gaaagagaac atggcgctgg tgttaattac
    17461 ccatgacctg gcgctggtgg cggaagcggc acataaaatc atcgtgatgt atgcaggcca
    17521 ggtggtggaa accggtgatg cgcacgccat cttccatgcg ccgcgtcacc cgtatactca
    17581 ggcattgctg cgtgcgctgc cagaatttgc tcaggacaaa gaacgtctgg cgtcgttgcc
    17641 aggtgtcgtt cccggtaagt acgaccgccc gaacggctgc ctgcttaacc cgcgctgccc
    17701 ctatgccact gacagatgtc gcgctgaaga accggcgctg aatatgctcg ctgatgggcg
    17761 tcagtccaaa tgccattacc cacttgatga tgccgggagg ccgacactat gagtacgcaa
    17821 gaggccacct cgcaacaacc gctgttgcag gctatcgacc tgaaaaaaca ttatccggtg
    17881 aagaaaggca tgttcgcgcc ggaacgtctg gtgaaggcgc tggacggcgt ttcgtttaac
    17941 cttgaacgtg gcaaaacgct ggcagtagtg ggcgaatctg gctgcggtaa atcgaccctc
    18001 ggtcggttgc tgacgatgat cgaaacgccc accggtggtg agctgtatta ccaggggcag
    18061 gatctgctca agcacgatcc gcaggcgcag aagctgcgtc ggcagaaaat ccagattgtc
    18121 ttccagaacc cttacggttc gctgaatccg cgtaaaaaag ttgggcaaat tctcgaagag
    18181 ccgctgctta ttaacaccag cttcagcaaa gaacagcgtc gggaaaaagc cctgtcgatg
    18241 atggcgaaag tcggcctgaa aaccgagcac tatgaccgct atccgcatat gttctccggc
    18301 ggacaacgtc agcgtatcgc tattgcccgt ggtctgatgc tcgacccgga cgtggtgatt
    18361 gccgatgaac cggtttctgc gctggacgtc tcagtgcgtg cgcaggtgct gaatctgatg
    18421 atggatttgc agcaggagtt ggggctttct tatgtcttta tctcccacga cctgtcggtt
    18481 gtggagcaca ttgctgatga agtgatggtg atgtacctgg gccgctgcgt ggagaaagga
    18541 acgaaggacc aaatcttcaa taacccgcgc catccgtaca cccaggcgct actctccgcg
    18601 acgccgcgcc tgaacccgga cgatcgccgc gaacgcatca aactcaccgg tgaactgcca
    18661 agcccgctga atccaccgcc gggttgcgcc ttcaacgccc gctgtcgtcg gcgcttcggc
    18721 ccctgcgccc agttgcagcc gcagctaaaa gactacggcg gtcaactggt agcttgtttt
    18781 gctgttgatc aggatgaaaa tccacagcgt taactcacaa aaccggggcc atgcgctccg
    18841 gtttactcaa tcagcttcaa cagtggcgac aagcatagca aaatgccgta cagcagaatc
    18901 agccaggctt taaatccgcg aagtttttcc agttgtgcga ctttatagat caggaaaaac
    18961 ggaatgaggc acgacacaat gccatataac gggcttccca actgaaagaa caccagcacc
    19021 gatacacgaa acgaaaccca aatcgtcaac gtaatgacga taaaagcgca gatcgccaga
    19081 gtcagcatgc gtgagttaat tttcttggta tcaataattc ggcttaacag attgagaata
    19141 atgcctttaa tggcctcgtg gaaaccgaga taaatgccaa agaatgcggt cagtacggca
    19201 aagatattaa gcaccgtaga ggtgatatga atgatatgcc cagggatcac ctgcgcggcc
    19261 agcgccagtg ccgagatatt ttgttcaaag gcagaaacgg cttcttcgtg gctaattgag
    19321 aaggtaaacg aaaaggcaaa aaacaggatc accgcgatga gcgtgatata actaattcgg
    19381 tgggtacgca gcgcgagccg ggttgccagt actttatccg cttcccgttt acgataggca
    19441 atattcattg ggttaagtac ctgaataaat accgcagaaa agaagcaaaa tggaatggta
    19501 agcaggacat cgcggaaaaa gacggaggct tgcgggaagg cggttatatt ggcgaaattc
    19561 cagtgcggga tcatcgcaaa accgaacacg acaataatcc ctactttgac caccaccatt
    19621 ggcccagaaa tcttaaacag taatcgttca ccaccagacg caatcgctac cagtacggcg
    19681 aaaatagcga ctttataaag tagagattgt gaaagatcgg catcggttaa accgaaggtt
    19741 ttcaggtacg aggcgctgtc gaaaaccacg gagagagagt agataaaaat cccgtggata
    19801 atcatcaaaa agtagataac cccgaggaaa attccccagt tcttccccag gtaatgactg
    19861 ataatatcgg tgtagtcatt acaggaatcg ctttcagaaa gggtttttaa ataaatgtcc
    19921 tgcactaccc aggtggcagg ataagcaatg atcgctgcgg taataaatac ccaaattccc
    19981 ttcaagccaa tttgtactgg catcagcacg gttccggcac caatcgccat gcctatgcat
    20041 aataaaaccc agccgaagtc gtagcgtgta aacggcaatt tctgattgtg tttcgatagt
    20101 gtgttgtgct gcataaagtc ctgtccagaa agaagtcggc ctcgcaaaaa tgaataaacc
    20161 cagtttctct tgcgcaacag gtggagaagt cgaatacgca gtatgtaaaa aatgaggcaa
    20221 cagattcact ggggaggtcg cgaggaggcg caattctaca aaattgcgca tttcactacc
    20281 agtctggagt cagcccggat atgaactgca actattgcaa ataaagaaat taagctggaa
    20341 ggcatcgcgc aatgaggaag attgccgggt gctgacagta aaaagcaatc gtaaaatcag
    20401 cggcaagccg gaagggttcc ggtgaggcgc aatgttgcgg gggctttatc cctggtggca
    20461 ttggttgctg gagagagaaa acccccgcac gttgcaggta tgcacctgac aacaccacgg
    20521 gggccattct tgaacctgaa caactcaaga atagccgcga aacgttgtca ttacaacaca
    20581 ggcggctata tgacgctcgc agaactgggc atggccttct ggcatgattt agcggctccg
    20641 gtcattgctg gcattcttgc cagtatgatc gtgaactggc tgaacaagcg gaagtaacgt
    20701 gtcatgcggg cgtcaggctg ccgtaatggc aatttgcgcc cggaccaggc cgcaggggtg
    20761 aaactctgcg gcctttttcg tgcttactgc gggtaaggca cccagtcgcc gccgttcagg
    20821 cgaacgtacg gtttatcctg gtattgaata actactgcat ttgagttctc ggagaccggt
    20881 gctgtttgtg gcaacccact ggtgagtttt ttccagtcaa cattgtcttc ggtgaaaatc
    20941 ttgccatcaa gaacgcgaac caccagatcg gagatagcca ggaagctgct cggttggtcg
    21001 atgacaatcg gtgccccctg atgtggtgcc ttcatgccga agaatttcac cccaacgggg
    21061 acgtctgtga tagacgggct agggatatca cgtaggccag atacctgcat tctgtcgccc
    21121 ttcagcgcgc cgccgtgttc cggcaccacg accaccatca ctttacgacc cgatttctcc
    21181 agttcagtaa agaaggcgtc cagttcatca aagaatttct gcgcccgcgc tttgtaatcc
    21241 gctgttttgc tgacccccgg ataatggttg ccgtcatgca gtggaagcgt gttgtagaac
    21301 gtggcactac ggctgttttt atctttttcg gtaacgtcca gccagcggtt aagcacggcg
    21361 gtatcgtcat aaaccggcga accatcaaag cccagcaaaa taaccggcag atttgtttga
    21421 tccatcaatt cagtctgcat gccgccattt tcgcgaactt ctttcaaaaa accgccgaac
    21481 tggccgttat gtcccatcat caggtgctgg gtaaagccca gtttcgaaag gttatcaaac
    21541 agatagcagt cgttatttgc cggttgatac agattagtgt gcgaagtctg cccgcagctg
    21601 gcgcgcagta aacggatcgc cgccgggcca ctgtaggagg tggcggagtt aaagttcttg
    21661 aactcaatat cgaaatgcga ccacagtgga tgcgacatca acccggcggc ttctatatcc
    21721 gaccaggaaa gcgaacagat gttaatcacc agtagttcaa atggctgagc atcagcgggc
    21781 agcgaagacg ggaaggtcga tttacgtttc gcctccgcgt tatagaaatt attcagccag
    21841 gcgttaaggt tcgccgttgt tggcggtgca gtttgtgcgg gcatatcacc cactaccggt
    21901 gcgccacccg tcgccgcaac ggttgccgct gcgttaccac ccgtcgttgt tacagtggtc
    21961 gtcggttgtc cggctggcca caaggagaaa cttggtcccg ccagggtaag tacgttcagc
    22021 catagcagta tggcaaccac aaaaacggta atgcgaatcc attgtgacag gaataaccag
    22081 gccactaata aaacaaaaat ggccccaatc atctgccagt taataaagcg tgtgacaagg
    22141 tcgattaaat aatcggtact gaaccccgcc acctgcgaac cctggctcat tatgctttcc
    22201 gggccaggca accaagtgtc atgccagaac aaagcaaagc cgatcggcag ggcaatccag
    22261 tggcgcaagc gatgcaggct gtagcgcgga atgggcatca gcagaaacgc ggcaaacacc
    22321 aaattgagga gcggatggaa gttaagatat cccgcccaca acaggccgaa cttaaccaga
    22381 aaatagaagt tccagccgga aaggccgcgc cagtattgcc agagggaaga aggcatggcg
    22441 gtattttgcg taaattgagt cattttttgg ttgccctggc tttttccgtg cggcgtaacg
    22501 tcccggccgg tttaacataa cgaggtttag caaagaacaa acgtaaggtg tcgcgaatgc
    22561 gtcgcaaaga gtgccgcgcc agatagccca gcgggaaaaa tatcagtgcg caaacgacaa
    22621 taatttcaat gatatcgctg atggtcatca tgatgagcgc tccacagcat catctaacag
    22681 tcgcatgggt tctggtattc gtcgccagtg gcgaccatcg tgctcggcat tgatgaccgg
    22741 tttagaactt tgcgttaaag gcagcggcat gccccattgt tctggggcaa gcaagcgcat
    22801 ctgcaccagc tcggcactga tttgatcatc ttcaaaccag accatacggt ttgagaaaat
    22861 gtcgccagta ggcaatggga aaatatgatt caacgcggta tccagatcgt taatccgaca
    22921 gaatgagaga aacagcacca gccgattacc gccaatggtc atgatatcgc cggtacggtt
    22981 agggcgacac agcgtcaggg cttgttcaac gcggataccc ggtaccggac gtagggcaac
    23041 cagaacgcct ttaccgtggg caggtaatag agggttattc atcatgttgt tgacggcatt
    23101 acagaacaca tcccacttct ggaaaccacg cagtttgagc ggctgggtca ttgacagcaa
    23161 ggtagtgata tcttccggca catagcgact aaacttctgc ccttgcacgc tttcgatcat
    23221 cgtcagacaa cgggagagtg gcgcattcca cggaataacc atatttgcac cgcaggccaa
    23281 taacaaacgt tcatcggtgg cgcgcaggct agcggtattt tcccgcacga ggattttcat
    23341 cgcactaccg cgctggcgac gcagggtatg aatgctgcgg gccagtggct cgatttgcgc
    23401 attttgctgt aaagaaaaga ccaccgtcgc cgcctgagcg gtacgggctt cattgaacag
    23461 gacttcgtta ttgttgaaca gttgccagtg ttccgatagc ggcggcgcac cttccagtac
    23521 agcaacatta ctcagaatgc gtttttcgtc gctgcgtggt tggatctccg cctcttcgct
    23581 ttgaactaat gtccagatac cattttgctg ctgaacgcta agctgctgac gggcgctgac
    23641 ccctttttcg ttgcaccaga aggcaatatc cagcaaatgt tggtcaccct gaaaacgcaa
    23701 actggcaaga ccaaaaagtg aacggtattc ctcaagcaac aatgaaaatt gtttatcgtt
    23761 attatttccg ggattaatta ccaaaagcga acaatggttt aacctgctcc atttattcat
    23821 tttatccaac catgagcgaa gccgctcggc aggaatgttt tgccatgcgt tatttgcgca
    23881 aacaagaata aaaaggtaat tatggggatc aatagaacac tgcaaatcac gggtcaaata
    23941 gtatagacct ttttcatgat ttagcattga aaataatttt attttttccg gcccttgata
    24001 atcatctaat tgaaagattt tcgccggatc gctgtccatg ctaatgaccg cgacgtgtgc
    24061 ggtttcagcc tgggatgcaa ttgtttgatt cgccagactg atagcatctt catggcgatc
    24121 gacgttaaac caccagactc cgcctgctgg catatgtcgc agctcatccc ataatgatga
    24181 gataccgata gagaatacag ggtccacaat gtccctcatg tcaccatttt gtctgattat
    24241 cagtttacta gcgaaagcta agaaataaac ctaccattga aattaaaact tatcagattt
    24301 agcatgtgaa tcaatttaga aggtcacaag acgtgtgatt ttagtctttt cgtttttaac
    24361 tttttctgtt gcgtaacgga aagtcaaaaa gtgagcaaat tcccgtctcg ccgtaaacaa
    24421 taaaccggcg aaatgctcac ggttagaatt gtctcatgaa cggtacgttt atttcatagg
    24481 gatcaagcaa aatgaataac aatgaaccag atactctgcc tgatcccgcg ataggctata
    24541 tcttccagaa tgatattgtg gcgttaaagc aggcattttc actgcctgat attgattatg
    24601 ccgatatttc ccaacgcgaa cagttggccg cggcattaaa acgctggccg ttgctggcag
    24661 agtttgcgca acaaaagtag gggattggtg aatggcc
//
LOCUS       DAZKHC020000066        24451 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZKHC020000066 DAZKHC020000000
VERSION     DAZKHC020000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.066"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            66..1043
                     /gene="ybeQ"
                     /locus_tag="ACHOSM_RS15485"
                     /old_locus_tag="pgaptmp_003097"
     CDS             66..1043
                     /gene="ybeQ"
                     /locus_tag="ACHOSM_RS15485"
                     /old_locus_tag="pgaptmp_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HGU4559572.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIIGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNGQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVAWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECSDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIREIEDLL"
     gene            complement(1113..1595)
                     /gene="ybeL"
                     /locus_tag="ACHOSM_RS15490"
                     /old_locus_tag="pgaptmp_003098"
     CDS             complement(1113..1595)
                     /gene="ybeL"
                     /locus_tag="ACHOSM_RS15490"
                     /old_locus_tag="pgaptmp_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HGU4559573.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1831..4413
                     /gene="leuS"
                     /locus_tag="ACHOSM_RS15495"
                     /old_locus_tag="pgaptmp_003099"
     CDS             1831..4413
                     /gene="leuS"
                     /locus_tag="ACHOSM_RS15495"
                     /old_locus_tag="pgaptmp_003099"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGU4559574.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALIQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRGKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4428..5009
                     /gene="lptE"
                     /locus_tag="ACHOSM_RS15500"
                     /old_locus_tag="pgaptmp_003100"
     CDS             4428..5009
                     /gene="lptE"
                     /locus_tag="ACHOSM_RS15500"
                     /old_locus_tag="pgaptmp_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HGU4559575.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            5009..6040
                     /gene="holA"
                     /locus_tag="ACHOSM_RS15505"
                     /old_locus_tag="pgaptmp_003101"
     CDS             5009..6040
                     /gene="holA"
                     /locus_tag="ACHOSM_RS15505"
                     /old_locus_tag="pgaptmp_003101"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGU4559576.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6042..6683
                     /gene="nadD"
                     /locus_tag="ACHOSM_RS15510"
                     /old_locus_tag="pgaptmp_003102"
     CDS             6042..6683
                     /gene="nadD"
                     /locus_tag="ACHOSM_RS15510"
                     /old_locus_tag="pgaptmp_003102"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGU4559577.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6707..7318
                     /gene="cobC"
                     /locus_tag="ACHOSM_RS15515"
                     /old_locus_tag="pgaptmp_003103"
     CDS             6707..7318
                     /gene="cobC"
                     /locus_tag="ACHOSM_RS15515"
                     /old_locus_tag="pgaptmp_003103"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HGU4559578.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7578..7895
                     /gene="rsfS"
                     /locus_tag="ACHOSM_RS15520"
                     /old_locus_tag="pgaptmp_003104"
     CDS             7578..7895
                     /gene="rsfS"
                     /locus_tag="ACHOSM_RS15520"
                     /old_locus_tag="pgaptmp_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGU4559579.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7899..8366
                     /gene="rlmH"
                     /locus_tag="ACHOSM_RS15525"
                     /old_locus_tag="pgaptmp_003105"
     CDS             7899..8366
                     /gene="rlmH"
                     /locus_tag="ACHOSM_RS15525"
                     /old_locus_tag="pgaptmp_003105"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGU4559580.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSTLTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8397..10298
                     /gene="mrdA"
                     /locus_tag="ACHOSM_RS15530"
                     /old_locus_tag="pgaptmp_003106"
     CDS             8397..10298
                     /gene="mrdA"
                     /locus_tag="ACHOSM_RS15530"
                     /old_locus_tag="pgaptmp_003106"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HGU4559581.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10301..11413
                     /gene="mrdB"
                     /locus_tag="ACHOSM_RS15535"
                     /old_locus_tag="pgaptmp_003107"
                     /gene_synonym="rodA"
     CDS             10301..11413
                     /gene="mrdB"
                     /locus_tag="ACHOSM_RS15535"
                     /old_locus_tag="pgaptmp_003107"
                     /gene_synonym="rodA"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HGU4559582.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11424..12512
                     /gene="rlpA"
                     /locus_tag="ACHOSM_RS15540"
                     /old_locus_tag="pgaptmp_003108"
     CDS             11424..12512
                     /gene="rlpA"
                     /locus_tag="ACHOSM_RS15540"
                     /old_locus_tag="pgaptmp_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HGU4559583.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12651..13862
                     /gene="dacA"
                     /locus_tag="ACHOSM_RS15545"
                     /old_locus_tag="pgaptmp_003109"
     CDS             12651..13862
                     /gene="dacA"
                     /locus_tag="ACHOSM_RS15545"
                     /old_locus_tag="pgaptmp_003109"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HGU4559584.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13973..14236
                     /gene="ybeD"
                     /locus_tag="ACHOSM_RS15550"
                     /old_locus_tag="pgaptmp_003110"
     CDS             13973..14236
                     /gene="ybeD"
                     /locus_tag="ACHOSM_RS15550"
                     /old_locus_tag="pgaptmp_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HGU4559585.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14337..14978
                     /gene="lipB"
                     /locus_tag="ACHOSM_RS15555"
                     /old_locus_tag="pgaptmp_003111"
     CDS             14337..14978
                     /gene="lipB"
                     /locus_tag="ACHOSM_RS15555"
                     /old_locus_tag="pgaptmp_003111"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HGU4559586.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWQPEATTNNIAPRLLQNILALLNNPDFEYITA"
     gene            15237..16190
                     /gene="ybeF"
                     /locus_tag="ACHOSM_RS15560"
                     /old_locus_tag="pgaptmp_003112"
     CDS             15237..16190
                     /gene="ybeF"
                     /locus_tag="ACHOSM_RS15560"
                     /old_locus_tag="pgaptmp_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HGU4559587.1"
                     /translation="MDSNNQIEPCFSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16399..17364
                     /gene="lipA"
                     /locus_tag="ACHOSM_RS15565"
                     /old_locus_tag="pgaptmp_003113"
     CDS             16399..17364
                     /gene="lipA"
                     /locus_tag="ACHOSM_RS15565"
                     /old_locus_tag="pgaptmp_003113"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HGU4559588.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17465..17668)
                     /gene="tatE"
                     /locus_tag="ACHOSM_RS15570"
                     /old_locus_tag="pgaptmp_003114"
     CDS             complement(17465..17668)
                     /gene="tatE"
                     /locus_tag="ACHOSM_RS15570"
                     /old_locus_tag="pgaptmp_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HGU4559589.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17797..18585)
                     /locus_tag="ACHOSM_RS15575"
                     /old_locus_tag="pgaptmp_003115"
     CDS             complement(17797..18585)
                     /locus_tag="ACHOSM_RS15575"
                     /old_locus_tag="pgaptmp_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HGU4559590.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            18678..19061
                     /gene="crcB"
                     /locus_tag="ACHOSM_RS15580"
                     /old_locus_tag="pgaptmp_003116"
     CDS             18678..19061
                     /gene="crcB"
                     /locus_tag="ACHOSM_RS15580"
                     /old_locus_tag="pgaptmp_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGU4559591.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(19116..19325)
                     /gene="cspE"
                     /locus_tag="ACHOSM_RS15585"
                     /old_locus_tag="pgaptmp_003117"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19116..19325)
                     /gene="cspE"
                     /locus_tag="ACHOSM_RS15585"
                     /old_locus_tag="pgaptmp_003117"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU4559592.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(19500..20060)
                     /gene="pagP"
                     /locus_tag="ACHOSM_RS15590"
                     /old_locus_tag="pgaptmp_003118"
     CDS             complement(19500..20060)
                     /gene="pagP"
                     /locus_tag="ACHOSM_RS15590"
                     /old_locus_tag="pgaptmp_003118"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HGU4559593.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            20649..22034
                     /gene="dcuC"
                     /locus_tag="ACHOSM_RS15595"
                     /old_locus_tag="pgaptmp_003119"
     CDS             20649..22034
                     /gene="dcuC"
                     /locus_tag="ACHOSM_RS15595"
                     /old_locus_tag="pgaptmp_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU4559594.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(22075..22755)
                     /gene="dpiA"
                     /locus_tag="ACHOSM_RS15600"
                     /old_locus_tag="pgaptmp_003120"
                     /gene_synonym="citB"
     CDS             complement(22075..22755)
                     /gene="dpiA"
                     /locus_tag="ACHOSM_RS15600"
                     /old_locus_tag="pgaptmp_003120"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HGU4559595.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(22724..24382)
                     /gene="dpiB"
                     /locus_tag="ACHOSM_RS15605"
                     /old_locus_tag="pgaptmp_003121"
     CDS             complement(22724..24382)
                     /gene="dpiB"
                     /locus_tag="ACHOSM_RS15605"
                     /old_locus_tag="pgaptmp_003121"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HGU4559596.1"
                     /translation="MLQFNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT         6128 a         6179 c         6048 g         6096 t
ORIGIN      
        1 tcttacgcaa aaatggaaca cataaaaata taatatgctt tatctgcaac cataaggaaa
       61 tgataatgat ttttacgtca agttgctgcg ataatttatc aatagatgag attatcgaac
      121 gtgctgaaaa aggcgattgc gaggctcaat atattattgg gttttattat aatcgcgata
      181 gcgcaattga ctctccggac gacgaaaagg ccttttactg gctgaagctg gccgctgagc
      241 aaggtcattg tgaagcacaa tattccttag ggcaaaagta taccgaggat aaaagccgtc
      301 ataaagataa tgggcaagcc atcttttggc tgaaaaaagc tgccctacaa ggccatactt
      361 tcgcttccaa cgcccttggc tggatactgg atcgtggaga agaccccaac tataaagaag
      421 cggttgcctg gtatcagata gccgcggaga gcggaatgtc ttatgcgcaa aataatcttg
      481 ggtggatgta cagaaatggc aacggagtcg cacaagacta tacgctggca tttttctggt
      541 acaaacaagc tgcattacaa ggccatagtg acgcgcaaaa caatctggcc gatctttatg
      601 aagacggaaa aggcgttgct caaaacgaga cactcgccgc attctggtat ttgaaaagcg
      661 cacagcaggg taatcggcac gcccagtttc aaattgcatg ggattataac gctggcgaag
      721 gggtggacca ggactataag caagcgatgt actggtatct gaaggctgct gctcagggga
      781 gcgtcggcgc ttacgtcaac atcggttata tgtataaaca cggacaaggc gttgagaaag
      841 attatcaggc cgcctttgaa tggtttacga aagccgctga atgcagtgat gccactgcct
      901 ggtataacct ggccattatg tatcactacg gagaaggaag acccgtcgat ctccgacagg
      961 ctctcgacct gtatcgtaaa gttcagtcat ccggaaccag agatgtcagt caagaaattc
     1021 gtgagattga agatttactg tagagataaa aacaaggacc ggggaatttc attcggccca
     1081 tattctcttt attgctgtgc gaaatcagac tattacggct cgaacgggcg tctctggaac
     1141 tgatcatgac cacatttcgg gcatagcgtc agcacttccg gtgtgtagat cgggagatgg
     1201 aagtgacatt tctcgcagac cagatttccc agccctacca cttctccgct gtgataaacc
     1261 ccgtgatgat tgaggtcctg gaaaacttcg cgccattcaa gctgcgtttt atcggtgatg
     1321 tctgccagct cctgccacaa gctttcttta atcacccgca taaagacgct gtcagattct
     1381 tctttcaggc tctcttcata gctcatggcg aactcttcca ggtcacgtct gacagctcgc
     1441 gtcagctcat caacctcggt tcgcgttaac tcccctgttt ttattacgcg ctcgcgcgcc
     1501 tgttccacca gtgcgtcgat atcacgttcg ccattgcgca gacgttcgct cagtgacgcg
     1561 accagttcac ggtaatattg agcaaccttg ttcatcgttt catctcccgg gtcgttaact
     1621 gactataata atagacgttc tttgttcctt gctttgctaa tacgcccaca aactctgtaa
     1681 attaatgtat gaataatccg ggcatcattt caacgtcgag tgcgcgaaag gctgttttga
     1741 cgcggacgtt tgggctatgc tatgcggatc tgaaaaacca cctctaacgc tacttttgta
     1801 gccgtaatga aaacaggacc actggctgcc atgcaagagc aataccgccc ggaagagata
     1861 gaatccaaag tacagcttca ttgggatgag aagcgcacat ttgaagtaac cgaagacgag
     1921 agcaaagaga agtattactg cctgtctatg cttccctatc cttctggtcg actacacatg
     1981 ggccacgtac gtaactacac catcggtgac gtgatcgccc gttaccagcg catgctgggc
     2041 aaaaacgttc tgcagccgat cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg
     2101 gtgaaaaaca acaccgctcc ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac
     2161 cagctcaaaa tgctgggctt tggttatgac tggagccgcg agctggcaac ctgtacgccg
     2221 gaatactacc gttgggaaca gaaattcttc accgagctgt ataaaaaagg cctggtatat
     2281 aagaagactt ctgcggtcaa ctggtgcccg aacgaccaga ccgtactggc gaacgaacaa
     2341 gttatcgacg gctgctgctg gcgctgcgat accaaagttg aacgtaaaga gatcccgcaa
     2401 tggtttatca aaatcaccgc ttacgctgac gagctgctca acgatctgga taaactggat
     2461 cactggccag acaccgttaa aaccatgcag cgtaactgga tcggtcgttc cgaaggcgta
     2521 gaaatcactt tcaacgttaa cgactatgac aacacgctga ccgtttacac tacccgcccg
     2581 gacaccttta tgggttgtac ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa
     2641 gcggcggaaa ataatcctga actggcggcc tttattgacg aatgccgtaa caccaaagtt
     2701 gccgaagctg aaatggcgac gatggagaaa aaaggcgtcg atactggctt taaagctgtt
     2761 cacccattaa cgggcgaaga aattcccgtt tgggcagcaa acttcgtatt gatggagtac
     2821 ggcacgggcg cagttatggc ggttccgggt cacgaccagc gcgactacga gtttgcctct
     2881 aaatacggcc tgaacatcaa accggttatc ctggcagctg acggctctga gccagatctt
     2941 tctcagcaag ccctgactga aaaaggcgtg ctgttcaact ctggcgagtt caacggtctt
     3001 gaccatgaag cggccttcaa cgccatcgcc gataaactga ctgcgatggg cgttggcgag
     3061 cgtaaagtga actaccgcct gcgcgactgg ggtgtttccc gtcagcgtta ctggggtgcg
     3121 ccgattccga tggtgacgct ggaagacggt accgtaatgc cgaccccgga cgaccagctg
     3181 ccggtgatcc tgccggaaga tgtggtgatg gacggcatta ccagcccgat taaagcagat
     3241 ccggagtggg caaaaactac cgttaacggt atgccagcgc tgcgtgaaac cgacactttc
     3301 gacaccttta tggagtcctc ctggtactat gcgcgctaca cttgcccgga gtacaaagaa
     3361 ggtatgctgg attccgaagc ggctaactac tggctgccgg tggatatcta cattggtggt
     3421 atcgaacacg ccattatgca cctgctctac ttccgcttct tccacaaact gatgcgtgat
     3481 gcaggcatgg tgaactctga cgaaccagcg aaacagttgc tgtgtcaggg tatggtgctg
     3541 gcagatgcct tctactatgt tggcgaaaac ggcgaacgta actgggtttc cccggttgat
     3601 gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag cgaaagatgc ggcaggccat
     3661 gaactggttt ataccggcat gagcaaaatg tccaagtcga agaacaacgg tatcgacccg
     3721 caggtgatgg ttgaacgtta cggcgcggac accgttcgtc tgtttatgat gtttgcttct
     3781 ccggcggata tgactctcga atggcaggaa tccggcgtgg aaggggctaa ccgcttcctg
     3841 aaacgtgtct ggaaactggt gtacgagcac acagcaaaag gtgatgttgc ggcactgaac
     3901 gttgatgcgc tgactgaaga tcagaaagcg ctgcgtcgcg atgtgcataa aactatcgct
     3961 aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca ccgcaattgc ggcgattatg
     4021 gagctgatga acaaactggc gaaagcacca accgatggcg agcaggaccg cgccctgatt
     4081 caggaagcac tgctggccgt tgtccgtatg cttaacccgt tcaccccgca catctgcttc
     4141 acgctgtggc aggaactgaa aggcgaaggc gatatcgaca acgcgccgtg gccggttgct
     4201 gacgaaaaag cgatggtgga agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc
     4261 cgtggcaaaa tcaccgttcc ggtggacgca acggaagaac aggttcgcga acgtgctggc
     4321 caggaacatc tggtagcaaa atatcttgat ggcgttaccg tgcgtaaagt gatttacgta
     4381 ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg aggaagcgtg cgatatctgg
     4441 caacattgtt gttatctctg gcggtgttaa tcaccgccgg gtgtggctgg catctgcgtg
     4501 ataccacgca ggttccttcc actatgaagg tcatgatcct ggactcaggc gatccgaacg
     4561 ggccattaag ccgtgcggtg cgtaaccagt tacgtctgaa tggtgtcgag ttgcttgata
     4621 aagaaaccac gcgtaaggac gttccatccc tgcgtttggg taaagtgagc atcgcgaaag
     4681 ataccgcatc ggtattccgt aacggtcaaa cagcagagta tcagatgatc atgacggtga
     4741 atgcgaccgt gttgatcccc ggccgtgata tctacccgat tagcgccaaa gtcttccgtt
     4801 cgttctttga taacccgcaa atggcgttag cgaaagataa cgaacaagac atgatcgtaa
     4861 aagagatgta cgaccgtgct gccgaacagc tgattcgtaa gctgccaagc atccgtgctg
     4921 cggatattcg ttccgacgaa gaacagacgt cgaccacaac ggatactccg gcaacgcctg
     4981 cacgcgtctc caccacgctg ggtaactgat gattcggttg tacccggaac aactccgcgc
     5041 tcagctcaat gaagggctgc gcgcggcgta tcttttactt ggtaacgatc ctctgttatt
     5101 gcaggaaagc caggacgctg ttcgtcaggt agctgcggca caaggattcg aagaacacca
     5161 cactttttcc attgatccca atactgactg gaatgcgatc ttttcgttat gccaggccat
     5221 gagtctgttt gccagtcgac aaacgctatt gctattgtta ccagaaaacg gaccgaatgc
     5281 ggcgatcaat gaacaacttc tcacactcac cggacttctg catgacgacc tgctgttgat
     5341 cgtccgcggt aataaattaa gcaaagcgca agaaaatgcc gcctggttta ctgctcttgc
     5401 gaatcgcagc gtgcaggtga cctgtcagac accggagcag gctcagcttc cccgctgggt
     5461 tgctgcgcgc gcaaaacagc tcaacttaga actggatgac gcggcgaatc aggtgctctg
     5521 ctactgttat gaaggtaacc tgctggcgct ggcccaggca ctggagcgtt tatcgctact
     5581 ctggccggac ggcaaactga cattaccgcg cgttgaacag gcggtgaatg atgccgcgca
     5641 tttcacccct tttcattggg ttgatgcttt gttgatggga aaaagtaagc gcgcgttgca
     5701 tattcttcag caactgcgtc tggaaggcag cgagccggtt attttgttgc gcacattaca
     5761 acgtgaactg ttgttactgg ttaacctgaa acgccagtct gcccatactc cactgcgtgc
     5821 gttgtttgat aagcatcggg tatggcagaa ccgccggggc atgatgggcg aggcgttaaa
     5881 tcgcttaagc cagccgcagt tacgtcaggc cgtgcaactc ctgacacgaa cggaactcac
     5941 cctcaaacaa gattacggtc agtcagtgtg ggcagagctg gaagggttat ctcttctgtt
     6001 gtgccataaa cccctggcgg acgtatttat cgacggttga tatgaaatct ttacaggctc
     6061 tgtttggtgg cacctttgat ccggtgcact atggtcatct taaacccgtg gaaacgctgg
     6121 cgaatttgat tggtctgacg cgggtcacaa tcatccctaa taatgttcct ccgcatcgtc
     6181 cccagccgga agcgaacagc gtgcagcgta aacacatgct tgaattggcg attgccgaca
     6241 agccattatt tactcttgat gaacgcgagc taaagcgcaa tgccccctct tacactgcgc
     6301 aaacactgaa agagtggcgg caggaacaag gaccggacgt gccgctggcg tttattattg
     6361 gtcaggattc actgctgacc tttccgacct ggtacgaata cgaaacgata ctcgacaatg
     6421 cacatttgat cgtctgtcgg cgtccaggtt acccacttga aatggcgcaa ccgcaatacc
     6481 agcaatggct ggaagatcat ttgacacata acccggaaga tcttcacctt cagcctgccg
     6541 gtaaaattta tctggctgaa acgccgtggt ttaacatctc ggcgaccatc atccgcgaac
     6601 gtttgcaaaa cggtgaatcg tgtgaggatt tattgccgga accggtactg acttacatta
     6661 accaacaagg cttgtatcgc tgatacctgt cgttacggag ggcatgatgc gactgtggtt
     6721 aattcgtcat ggtgaaacgc aagcgaatat cgatggtctt tacagcggtc atgcgcccac
     6781 ccccctgacc gcgcgcggta ttgagcaagc gcaaaatctg catacgctgc tacatggtgt
     6841 ttcctttgat ctggttttat gcagtgaact ggaacgggca cagcataccg cgcgactggt
     6901 tctcagtgac cgccagctcc ccgtgcaaat catccctgaa ctcaacgaaa tgttttttgg
     6961 cgactgggag atgcgacatc atcgcgacct catgcaagaa gatgccgaaa actatagcgc
     7021 gtggtgcaat gactggcagc atgcaatccc cacgaacggt gaaggatttc aggcattttc
     7081 gcaacgtgtg gaacgcttta tcgcaaggct tagtgaattt cagcactatc agaatatttt
     7141 agtcgtcagc catcagggtg tactgagtct gttaatcgcc cgtttaattg gcatgcctgc
     7201 cgaggctatg tggcattttc gcgttgacca ggggtgttgg agcgccattg atatcaacca
     7261 aaaattcgcg acgctacgcg tcctcaatag ccgtgccatc ggggtcgaga atgcatgact
     7321 tttctgtttt tttacgggta agccgcaacg accattgaca gacccgggca ggctgatatt
     7381 ctccgcagcc agactttttt cgccagacac gactttgtag aaattgtttt acaaaaatgg
     7441 cgatgcaatc tgcggcgcgg ggtgggatga tagcccactt tcgaaagccg attcggcgac
     7501 aattgtcccg aaatcgcctc tggttcaggt atactgacag accattttta tctatttgat
     7561 tcacccaggg ggaaaacttg cagggtaaag cactccagga ttttgttatc gacaaaattg
     7621 atgacctcaa aggtcaggac atcatcgcct tagacgttca aggcaaatcc agcatcaccg
     7681 actgcatgat catctgtacg ggtacgtcca gccgtcatgt tatgtccatt gctgaccacg
     7741 ttgtgcagga gtctcgcgca gcgggcctgt taccgctcgg cgtagaaggt gaaaacagcg
     7801 ccgactggat tgtcgtggat ttgggcgatg tgattgtcca tgtcatgcag gaagagagcc
     7861 gtcgcctgta tgaactggaa aaactctgga gttaatgcgt gaagctgcaa cttgtcgccg
     7921 tgggaacgaa aatgccggac tgggtacaaa ccggttttac cgagtacctg cgtcgttttc
     7981 cgaaagatat gcccttcgag ctgattgaaa ttccggccgg aaaacgcggc aagaatgcgg
     8041 acatcaagcg catactcgac aaagagggtg agcagatgtt ggcggccgca ggcaaaaacc
     8101 gcattgtcac cctcgatatt ccaggcaagc cctgggatac gccgcagtta gccgctgagc
     8161 tggaacgctg gaagctggat ggtcgcgacg tcagtctact gattggcggg cctgaagggt
     8221 tgtcgcctgc ctgtaaagcg gcggcggagc agagctggtc gctgtcgacg cttaccctcc
     8281 cccatccgct ggttcgcgtg ctggtcgcag agagtctgta ccgggcgtgg agcatcacca
     8341 ccaaccatcc ttatcaccgt gagtgataag ggagctttga gtagaaaacg cagcggatga
     8401 aactacagaa ctcttttcgc gactatacgg cagagtccgc gctgtttgtg cgccgggcgc
     8461 tggtcgcctt tttggggatt ttgctgctga ccggcgtgct tatcgccaac ctgtataatc
     8521 tgcaaattgt tcgctttacc gactaccaga ctcgctctaa tgaaaaccgc attaagctgg
     8581 tgcctatcgc gcccagccgc ggcattatct acgaccgtaa tggtatccct ctggccctca
     8641 accgcactat ctaccagata gaaatgatgc cagagaaagt cgataacgtg cagcaaacgc
     8701 tggacgcttt gcgcagcgtg gttgatctga ccgatgacga tattgctgca ttccgaaaag
     8761 agcgcgcacg ttcacaccgt ttcacctcta ttccggtgaa aactaacctg accgaagtac
     8821 aagtagctcg ctttgccgtc aatcagtacc gttttccggg tgtcgaagtt aaaggctata
     8881 aacgtcgtta ctatccttac ggttcggcgt tgacccacgt catcggctat gtgtcgaaaa
     8941 tcaacgataa agacgtcgaa cgcctgaata atgacggcaa actggccaac tatgcggcaa
     9001 cgcatgatat cggtaagctg ggcattgagc gttactatga agacgtgctg cacggtcaga
     9061 ccggttatga agaggttgaa gttaacaacc gtgggcgtgt tatccgccag ttaaaagaag
     9121 taccaccgca agccggacac gatatttacc tgacgctgga tctcaaactc cagcaatata
     9181 ttgaaacgct gctggcgggt agccgcgcag ctgtggttgt caccgatccg cgtacaggtg
     9241 gggtgctggc gctggtttcc acgcctagtt atgacccaaa cctgtttgtt gacggtatct
     9301 ccagcaaaga ttattccgcc ttgttgaacg acccgaatac accgctggtg aaccgcgcca
     9361 cacagggggt ttatcctccc gcgtctacag ttaaacccta tgtggcggtt tcggcattga
     9421 gcgccggggt gatcacgcgc aatacgacgc tgtttgaccc aggctggtgg caactgccag
     9481 gttcggaaaa acgttatcgt gactggaaaa aatggggcca cgggcgtctg aatgtcacaa
     9541 gatcgctgga agaatctgcg gataccttct tctatcaggt ggcctacgat atggggatcg
     9601 atcgcctctc cgaatggatg ggtaaattcg gttatggtca ttacaccggt atcgacctgg
     9661 cggaagaacg ttccggcaac atgcctaccc gcgaatggaa acagaaacgc tttaaaaaac
     9721 cgtggtatca gggtgacacc attccggttg gtatcggtca gggttactgg acagcgaccc
     9781 caatccagat gagtaaggca ctgatgatcc tgattaatga cggtatcgtg aaggttcctc
     9841 atttgctgat gagcaccgcc gaagacggca aacaggtgcc atgggtacag ccgcatgaac
     9901 cgcccgtcgg cgatattcat tccggttact gggagctggc gaaagacggt atgtacggtg
     9961 ttgctaaccg ccctaacggt acggcgcata aatactttgc cagcgcaccg tacaaaattg
    10021 cggcgaaatc cggtaccgct caggtcttcg gtctgaaagc gaacgaaacc tataatgcgc
    10081 acaaaattgc cgagcgttta cgtgaccaca aactgatgac cgcctttgcg ccatacaaca
    10141 atccgcaagt ggctgtcgcc atgattctgg agaacggtgg tgcgggtccg gcggttggta
    10201 cactgatgcg ccagatcctc gaccacatta tgctgggtga taacaacacc gatctgcctg
    10261 cggaaaatcc agcggttgcc gcagcggagg accattaatc atgacggata atccgaataa
    10321 aaaaacattc tgggataaag tccatctcga tcccacaatg ctgctgatct tactggcatt
    10381 gctggtttac agcgccctgg ttatctggag cgccagcggt caggatattg gcatgatgga
    10441 gcgtaaaatc ggccaaatcg cgatgggtct ggtcatcatg gtggtgatgg cgcaaattcc
    10501 tccacgcgtt tatgaaggct gggcccccta tctctatatc atctgtatta ttttgctggt
    10561 ggcggtagat gctttcggtg ccatctctaa aggtgctcaa cgctggctgg acctcggtat
    10621 tgttcgtttt cagccgtcgg aaattgccaa aatagccgta ccactgatgg ttgcgcgctt
    10681 tatcaaccgc gacgtttgcc cgccatcgtt gaagaacact ggcatcgcgc tggtgctgat
    10741 atttatgccc acgctgctgg tggctgcaca gcctgacctg ggaacatcaa tcctcgttgc
    10801 gctttccggt ctgtttgtac tgttcctctc tggccttagc tggcgtctga ttggcgtcgc
    10861 agtagtgctg gtggcggcgt tcattccgat tctgtggttc ttcctgatgc atgattacca
    10921 gcgccagcgc gtaatgatgc tcctggaccc ggaatcagac ccactcggcg cgggctatca
    10981 cattattcag tctaaaattg ctattggctc cggcggatta cgcggcaaag gctggctgca
    11041 cggcactcag tcacagcttg aatttctccc cgaacgccat accgacttta tcttcgcggt
    11101 actggcggaa gagctgggat tagtgggcat tctgattctg ctcgctctct acattctgct
    11161 gatcatgcgc gggctgtgga tagccgccag agcgcaaacc acctttggtc gcgtcatggc
    11221 tggtggctta atgctgatat tattcgttta tgtcttcgta aatattggta tggtaagcgg
    11281 tattctgccg gttgtagggg ttccgctccc actggtcagt tatggaggat cggcgctaat
    11341 tgtgctgatg gctgggttcg ggattgtaat gtcaatccac acccacagga aaatgttgtc
    11401 gaaaagcgtg taagaggtgc gcaatgcgta agcagtggct cgggatctgc atcgcggcag
    11461 gaatgctcgc ggcatgtaca agcgatgatg gtcagcaaca gacggtaagt gtaccgcagc
    11521 ctgcggtatg taacggcccc atagttgaaa ttagcggggc ggacccgcgt ttcgaaccac
    11581 tgaacgcgac ggcaaatcag gattaccagc gcgacggtaa aagctacaaa atcgtgcagg
    11641 atccgtctcg atttagccag gcgggactgg cggcaatcta tgatgccgaa cccggcagta
    11701 acctgacggc ctctggcgaa gctttcgatc cgacacagct gacggcggcc catccaacgc
    11761 ttccgatccc cagctacgcc agaatcacta acctggctaa cgggcgaatg atcgtggtgc
    11821 gcattaatga tcgcggccct tacggcaacg accgcgttat ttcgctttct cgcgcggcag
    11881 ctgaccgtct taacacgtca aacaacacca aagttcgtat cgatccgatt attgtcgccc
    11941 aggatggttc gctttctggt cctggtatgg cttgtaccac agtcgccaaa cagacttacg
    12001 ccctgcctgc acctcccgat ttaagcggtg gcgcgggaac aagttcagtg tctggcccgc
    12061 agggtgacat tcttccggtc agtaattcga cgctaaaaag cgaagatccg accggcgcgc
    12121 cggtaaccag tagcggtttc ctcggcgctc caacgacctt agcgcctggt gtactggaag
    12181 gcagcgaacc gacgcctgcg ccacagcccg ttgttacagc tccgtcgaca acgcctgcaa
    12241 cctcgcctgc aatggtgaca ccgcaagccg cctcgcaaag cgccagcggc aactttatgg
    12301 tgcaagtcgg ggccgtaagc gatcaggctc gtgcgcaaca gtaccaacag caactgggac
    12361 agaagttcgg cgtccccggt cgcgtaactc aaaatggcgc ggtctggcgg atccagcttg
    12421 gcccattcgc cagcaaagcc gaagccagta ccttgcagca acgtttgcaa accgaagccc
    12481 aattacagtc atttattact accgcgcagt agcgtaaagc aggcatctga tgtgtcaatt
    12541 tctgtaaatg acgttaacaa agtcacgcgg aaagtcagat gcctgccggt agtgcatttg
    12601 ctatagtagg gcactttttt aattccatca cggatgtcgt agttcagacc atgaatacca
    12661 ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta
    12721 tctctgctgc acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga
    12781 tcgatgcgga gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga
    12841 acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg
    12901 gccaggcaat gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg
    12961 catgggccac cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca
    13021 tgcaggttcc ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt
    13081 gtgtcgccat ggccgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca
    13141 gctacgttaa cgcactgggc ctgaaaaata cccacttcca gacggtacat ggtctggatg
    13201 ctgatggtca gtacagctcc gcgcgagata tggcgctgat cggccaggca ttgatccgtg
    13261 acgtaccgaa tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc
    13321 agctgaaccg taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg
    13381 gacacactga caaagcaggt tacaaccttg ttgcttctgc gactgaaggc cagatgcgct
    13441 tgatttctgc ggtgatgggc ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac
    13501 tgctaacctg gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt
    13561 tcgcctctga accggtttgg tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag
    13621 acgtgtacct gaccattccg cgtggtcgca tgaaagatct gaaagccagc tatgtgctga
    13681 acagcagtga attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc
    13741 agcttgatgg caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa atcccggaag
    13801 gtaacttctt cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt
    13861 aaaaattgaa cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaaa
    13921 ctcccgcctt ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac
    13981 caaacttaac gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc
    14041 gttacctgag ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta
    14101 caccccaacg gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa
    14161 cgccactcat atcgagcagg ttgaaacact gtatgaagaa ctgggcaaaa tcgatattgt
    14221 ccgcatggtt ctgtaactcg cttctccgtt acccggctcc tggtcgggta actcccctct
    14281 cctcgctgtg atatactttt cccccacttt tactcattct ccacggagat gccgttttgt
    14341 atcaggataa aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccagg
    14401 ctatgcatga attcaccgat acccgcgatg atagtaccct tgatgaaatc tggctggtcg
    14461 agcactatcc ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg
    14521 gtgatattcc ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccggggc
    14581 aacaggtgat gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg
    14641 tgaccttgct tgaacaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc
    14701 ctcgggctga cgcgccaggt gtctatgtcg gggaaaagaa aatttgctca ctgggtttac
    14761 gtattcgacg cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac
    14821 catttttacg tattaatcct tgtgggtatg ccggaatgga aatggctaaa atatcacaat
    14881 ggcaacccga agcgacgact aataatattg ctccacgttt actgcaaaat attttagcgc
    14941 tactaaacaa tcctgacttc gaatatatta ccgcttaatt ccatacatca atggcccaat
    15001 ttacactggg tcattactca taatacctgc taatgcttta catctccttc gcgtttgtat
    15061 attttcttta tcacttgcag aaatgcgatg ctacgccttg ctccgtaact caccccattg
    15121 attaacctat tgaaatagtt gattatggtg agcgaaagcg ttccgttcca attagacata
    15181 agattattgt aattttttaa atattatttt tccatgaata aaattggagt aagtgcgtgg
    15241 atagtaataa tcaaattgaa ccctgtttta gcagaaagtc gtcagaaggt aaaccacaaa
    15301 tatttacaac cttacgaaat attgatctta accttctgac tatttttgaa gctgtatatg
    15361 tacataaagg gatcgttaat gcagcgaaag tgcttaatct gaccccctcg gcaatcagtc
    15421 agtctattca gaaactgcgc gttatattcc ctgacccatt gtttattcgc aaaggccagg
    15481 gtgtcactcc taccgcattt gcgatgcatc tacatgagta tatcagtcag ggccttgagt
    15541 ccattcttgg cgcgctggat atcgaaggaa gctatgataa gcaacgaacg ataactattg
    15601 cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat ctatcgggcg attaaaactc
    15661 actatccgca gcttttactg cgcaacccgc ccatcagcga cgcggaaaac caactcagtc
    15721 agtttcaaac cgatctcatc atcgataaca tgttttgcac caatcgtacg gtgcaacatc
    15781 atgttctgtt caccgacaat atggtgttaa tttgccgcga gggaaatcca ctactctctt
    15841 tagaagatga cagagagact atcgacaacg ctgcgcatgt acttctgtta ccggaagagc
    15901 aaaatttcag cggtctgcgg cagagagttc aagaaatgtt tccggaccgg caaatcaatt
    15961 tcaccagcta caacattttg acaatcgctg cactggttgc caacagtgac atgttagcga
    16021 ttattcccag ccgtttttat aacctgttta gccgctgctg gccgctggaa aaactgcctt
    16081 ttccgtcctt aaatgaggag caaatagatt tctctatcca ctacaacaaa ttcagcctgc
    16141 gtgatcccat cctgcacgga gtaatagatg ttatccgtaa tgcattttga aaaagtattg
    16201 ttacagccag atcacagcgc agaacgataa aatggcacaa tccaacaaca attttacatt
    16261 ttcgcgaccg ctttggctgc tttcaggtcc gtttcaatga tatactgcca gtcgttaatt
    16321 caaaaatagt tgataattac aacaatctat tgaattgaaa cgctttcctt cgtaattcgc
    16381 aactggaaca cgcacgctat gagtaaaccc attgtgatgg aacgcggtgt taaataccgc
    16441 gatgccgata agatggccct tatcccggtt aaaaacgtgg caacagagcg cgaagccctg
    16501 ctgcgcaagc cggaatggat gaaaatcaag cttccagcgg actctacacg tatccagggc
    16561 atcaaagccg caatgcgcaa aaatggcctg cattctgtct gcgaggaagc ctcctgccct
    16621 aacctggcgg aatgcttcaa ccacggcaca gcaacgttta tgatcctcgg cgctatttgt
    16681 acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc cggtagctcc tgatgccaat
    16741 gaaccagtga aactggcgca gaccattgcc gatatggcgc tgcgttatgt ggttatcacc
    16801 tccgttgacc gtgatgacct gcgcgatggc ggtgcccagc actttgcgga ttgcattact
    16861 gccattcggg aaaaaagccc gcaaatcaaa attgaaactc tggtgccgga tttccgtggt
    16921 cgtatggatc gtgctctgga tattctgact gcaacgccac cagatgtgtt caaccataac
    16981 ctggaaaacg taccgcgtat ttaccgtcag gtacggcctg gtgcagatta caactggtcg
    17041 ctgaagctgc tggaacgctt taaagaagcg catccggaaa tcccgaccaa gtctggtctg
    17101 atggtgggac tgggtgaaac caatgaagaa attattgagg taatgcgcga cctgcgccgt
    17161 catggtgtga cgatgttaac gctggggcaa tatttgcagc caagccgcca tcacctgccg
    17221 gttcaacgtt acgttagccc ggatgagttc gacgaaatga aagccgaagc gctggcgatg
    17281 ggctttaccc atgctgcatg cggtccgttt gtccgctctt cttaccacgc cgatttgcag
    17341 gcaaaaggga tggaagttaa gtaatcctgt aataattact tacatctgtc tgataaaaaa
    17401 cccgcttttt gaagcgggtt ttttgtatcg aacaagatat tgagggagcg tcctgctcgc
    17461 cacgtcactc tttatgagag agcttttcag cctgaagatc aacgtctgcg ccttttttcg
    17521 ccgcagcatc atcatcattc atcgccttct tgaacccttt aatggccgct ccaaggtctc
    17581 cgcccagcgt acgtaacttc ttagtcccaa acagcagaac gaccagcgcc gcaactacca
    17641 gcagtttggt aatactaatc tcacccatag ataccttctt gacatataac cggcaacaga
    17701 acgcgcctta aaaaccagag aatattaacg gtcattcgac gcgcgcacac aatagcaatc
    17761 tgtaacaagg tgaatcaagc gccgagtaaa aaaacatcat aataattgcg gcggcgcaaa
    17821 gcgacggttg tttaagacgg gcagttgcgc gcgcacctga cgcacacgtt cgggcgtcac
    17881 ttccgccata atgagtgcag gcatttctga cgctgccgca atggtgacgc caaagggatc
    17941 tataatccgg ctttgaccga tatttttgtt cccgcactcc cccgccgcca ccatataaca
    18001 ggtggtatcc agcgcacgag cggcaagcaa cgttgaccag tgatgctctt tgagcggccc
    18061 gcgaacccag gcggcaggaa gtaccaggat ttcagctccc tgtaatgcct gtgccagcgc
    18121 cagctctgga aagcgtaagt cataacaggt catcagaccg accttcatcc cctccacctc
    18181 gagtaacgga gcgatttcat taccagcatc aacacggcgt gattcctgaa tggcaaatgc
    18241 atcatagaga tgcaatttgg cataacgggc gacgatgttt cctgcctgta gcgccaccag
    18301 catattccat gcgcgccccg gcgttgaagg aacatgaatc gtcagaattg tcgtcatcat
    18361 gttacgttta ctttctcgcc gtaaacgtcc gaggaattcg ccttccagca gctgtgctga
    18421 tttaaccgat agatctgcat catgatcatc gcgcgccagc aatgcttccg gcaggacgaa
    18481 cagcgatacg tcgttttccg ccgcctgcgc catcaacgag gcacaaatct cagcgttctt
    18541 ttcccacaca gatgtaacag caaactgtcc tgcggcaacc agcattgcca cctcctgtca
    18601 gagtcgtaaa attcgccaac gacgattatt cggcgttaca cttatcactc atacaaatca
    18661 aatagcagga ttttgcagtg ttacaacttc ttttagcagt ttttattggc ggtggtacgg
    18721 gaagcgtggc gagatggctg ttaagtatgc gatttaaccc actgcatcag gcgattccgt
    18781 tggggacgct gacagcaaac ctgattgggg cattcatcat aggaatggga ttcgcatggt
    18841 tcagcaggat gacgaacatt gatccagtgt ggaaagtatt aatcaccacc ggattttgtg
    18901 gcggtctaac aaccttctca acattttcgg cagaagtggt gtttttgtta caagagggtc
    18961 gctttggctg ggcattactg aacgttttcg tcaaccttct ggggtctttt gccatgacag
    19021 cactggcttt ctggctgttt tcggcctcaa ccgcacacta aagaccaaaa aaaaacccgc
    19081 tgattaagcg ggttttgaat tcttgctgac gtatcttaca gagcgattac gtttgcagca
    19141 gaagggcctt tggcaccgtt agtgatttcg aactctacgc gctgaccttc agcaagagtt
    19201 ttaaaaccat tagtctggat tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc
    19261 ggagtaatga aaccgaatcc tttggactca ttaaaccact taacgttacc tttaatctta
    19321 gacatcaaaa ttacctttac atgaaaaata gacacaaatg ctgtgtcggt taccagtaca
    19381 ccaattgtgg tacgctttgt ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat
    19441 ttttgatgtt acttatttta gctattgatt ttaaagaagt tactaaaact tcatttgtct
    19501 caaaactgaa agcgcatcca ggcaaagtac acattgccat tgttgtaggt acccggaatg
    19561 taggtcatct gaaaagtcac tgggccataa cccacggagg ccaatggcag tagaaccggg
    19621 agagggatgt aattccagtt atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa
    19681 tgaaaatttt catccgccag cggtcgccag gtactttccc atccgtatcc ggcaatcggt
    19741 tcccatttgt tccacgagtc cttaaatgcc atggcataca ggccatgcca gtttcctttt
    19801 tcatcccaac gcgacaggcc aaaaccgcca ccccacggtc gctcgttata gcgatcggtt
    19861 ttttctttgt cgtaagcgaa acgtgcatgc caggtgatgg caggaatata taaatcataa
    19921 tgttcaggct gttgccaggt ttgtgcaata ttttctctaa acgttgtcat ccactcatct
    19981 gcgttagcaa aaactttacc aacgctgatt aactgaataa aaacaaagga aaagatagcg
    20041 acatatttac tcacgttcat ttgtgaccat aaaacattta tcaaaaatct actactagca
    20101 tagcaaagct acaattaaca taaccttaat agacccaaca taaagaataa tctgaatagc
    20161 tgctctattt atttccgctt gttcaagtta aatatttccg cgcatcgatt aaagatgatg
    20221 cgtcgttttc tgcgatggga atagtcaaaa aagaaaaacc aagtcttttt tgatgacaaa
    20281 atgcaatcaa ggaaaaatta ttttattttt taacgagata caacaatcat cttaacgaag
    20341 tatataatat atatcgtcaa cctatgctca tattagatta ttttttgagc gcacagccgc
    20401 agctaacacc attgcaatta acaaatttgc atcaatccac catcaatttg cacacattat
    20461 tatgtgataa ttgccaaccg ctaaatatgc gttttgttat ctatgtataa aaacagcaac
    20521 ttcaatgtct taatggcagt ttttcttgat tttaatcagc attcatcgcc aatttattgg
    20581 gcatattttt tccttaagct ttaggaattt tttattattt actttggggc ctggagacag
    20641 gaaaaattat gctgacattc attgaactcc ttattggggt tgtggttatt gtgggtgtag
    20701 ctcgctacat cattaaaggg tattccgcca ctggtgtgtt atttgtcggt ggcctgttat
    20761 tgctgattat cagtgccatt atggggcaca aagtgttacc gtccagccag gcttcaacag
    20821 gctacagcgc cacggatatc gttgaatacg ttaaaatatt actaatgagc cgcggcggcg
    20881 acctcggcat gatgattatg atgctgtgtg gatttgccgc ttacatgacc catatcggcg
    20941 cgaatgatat ggtggtcaag ctggcgtcaa aaccattgca gtatattaac tccccttacc
    21001 tgctgatgat tgccgcctat tttgtcgcct gtctgatgtc tctggccgtc tcttccgcaa
    21061 ccggtctggg tgttttgctg atggcaaccc tatttccggt gatggtaaac gttggtatca
    21121 gtcgtggcgc agctgctgcc atttgtgcct ccccggcggc gattattctc gcaccgactt
    21181 caggggatgt ggtgctggcg gcgcaagctt ccgaaatgtc gctgattgac ttcgccttca
    21241 aaacgacgct gcctatctca attgctgcaa ttatcggcat ggcgatcgcc cacttcttct
    21301 ggcaacgtta tctggataaa aaagagcaca tctctcatga aatgttagat gtcagtgaaa
    21361 tcaccaccac tgctcctgcg ttttatgcca ttttgccgtt cacgccgatc atcggtgtac
    21421 tgatttttga cggtaaatgg ggtccgcaat tacacatcat cactattctg gtgatttgta
    21481 tgctgattgc ctccattctg gagttcctcc gcagctttaa tacccagaaa gttttctctg
    21541 gtctggaagt ggcttatcgc gggatggcag atgcgtttgc taacgtggtg atgctgctgg
    21601 ttgccgctgg ggtattcgct caggggctta gcaccatcgg ctttattcaa agtctgattt
    21661 ctatcgctac ctcgtttggt tcggcgagta tcatcctgat gctggtattg gtgattctga
    21721 ccatgctggc ggcagtcacg accggttcag gcaatgcgcc gttttatgcg tttgttgaga
    21781 tgatcccgaa actggcgcac tcttccggca ttaacccggc gtatttgact atcccgatgc
    21841 tgcaggcgtc aaacctgggt cgtaccctat cacccgtttc tggcgtagtc gttgcggttg
    21901 ccgggatggc gaaaatctca ccatttgaag tcgtaaaacg cacctcggtg ccggtgcttg
    21961 ttgggctagt gattgttatc gttgctacag agctgatggt gccaggaacg gcagcagcgg
    22021 tcacaggcaa gtaaatagta atgccggagg caagtttcct ccggctttat catgtcaccc
    22081 actgtggtat atgcgttgtg gtctgccaac tttgccgtga acaatttcag caataatcag
    22141 atggcggctg gcgcaatatt caagataacg cctggcagtg gtgcgactga tggtcagtgc
    22201 ctgcgccacc gtttctgccg tatgttgcac accaggctct ttaaacagtt ttcgcaccgc
    22261 gtttagcgtc aagggatcaa tgccggtcgg cagttcgtct ttaggttcac cgcgagcata
    22321 ggcattaaac atctcatcaa tttgcttctg gctggcgcta tcaatacttt ccagcatatg
    22381 tttacgctgg cggaaacggg ttagcgtttg ccccagccgt tcataggcaa tgggcttaat
    22441 gagataatca aatacaccac aacgtacggc ttcagacacc gtttccatat cgctggctgc
    22501 agtggtaaac accacgtcgc cgggataatg cgcctgcacc agttcatgca ataaattaat
    22561 ccctctgccg tcaggaagat agttatcgag caagattagc cccggcttaa aacgctcgat
    22621 catcattcgg gcctgcgcca ggtttcccgc cagtaatatc tgactgaatc cgggaatgtg
    22681 acgaatatat tccgcatgca tctctgccag cggcgtttcg tcctcaacga tcaatagggt
    22741 taatggagct gtcattaggt ttcactttcg gaatatagat tgaaaataag gtaccgcagg
    22801 gatcattatc ttcgagagtg ataacaccac cgcagcgcgt tacgtagctg gcaatcaagt
    22861 acaacccaat gccatgttca ccgggctcgt cagcacgcgt actgaccccc tgctcaaata
    22921 ttttgtctcg tagagactct ggaacaccgc agccctgatc ggcgacttca atcaccacat
    22981 catcgccttc atcgctgagg aataattcaa cgatcttgtt tccttcatcg ctacgcaggc
    23041 tggcttcgaa ggcgttatca agtaaattgc ccacaatggc tgcaaactcg gtgctatcca
    23101 gtcctggcgg cagttgcgaa agctggctac cggggacaat gatcattttt agccccagtt
    23161 cccgggcgcg ctgcacttta ccaaaaagca gccccgccac ctggcgatcg gcaaacgcct
    23221 cgcgcaggct gtcaataagc tgttgctggg cctgagactc cccctgcacc atcgccagca
    23281 cgcgatcata ctctttcatc tgcaacagac cattgagcgt cgacatccaa ttgagatgct
    23341 cgtgtcgcaa tgtacgaagg ctctcgacgt attgttttat ttgcgtcagt tgcgcattga
    23401 gggtggaaat ttcgtcttta ctacgaaagc tgataatggc ccccagcaaa tcatcacctg
    23461 aacgaatagc ttcccgattg gcaataacgc tcagaccgtt aaagttagcc accacatcct
    23521 gacgtttttc atcaatctgt tcggtaaaga aatcggcggg cctgaccact tcagcaatgg
    23581 gtttacccaa ccattgccgt ccgggagagc tcagccccag catctttctt gcgttacgat
    23641 tgatggcggt aatgtaacca tgcggatcca ccgcaatcag cccttcataa accgaactaa
    23701 acagcgcctc ttgctgacgg accacgcgtg cgatttgctt tggctccatg cccatcattt
    23761 gccgacggat atgcgctgcc aggaaccacg acagcaacat cagaatccct aacagcacga
    23821 caaacacgcc tgccatcggt aataaaaact cagcccgcca gctatcgatt ttactcacca
    23881 ggtagccaat cgacaccacg ccgatgactt ttccatcgtc atcaaagatt ggcgttttgg
    23941 cgcgcatcgc catccccatt gaccctttcc cggtaatgaa gtagctctcc cctttctcca
    24001 gcgcgccctg tttggtgaac tgcataggat aaccaatttt ctccggatta ggatggtaaa
    24061 ggcggatcga gtgccggtcc ccaatcacca cataatcaaa atcggtatct ctttgtaatt
    24121 tgttagcgat ggtcgccagc cgtttgtagt cacgcgtttt caccgcagag atgacactgt
    24181 cattggaggc aataattttc gcctgattca ttgccatgtc gcgtacatga agcgttaaat
    24241 agtcctcaaa actggccgta aaatattgcg ccagggctgc aatgacaaat attgagaaca
    24301 ccagaatcag caaaaagata cgcagcggaa atgccagtct ttggaaaaat gcaaactgtt
    24361 tattctcgtt aaactgcaac attgttttcc ttgcgggttg tcccgagcct ctttgacgga
    24421 tgtaattaaa atgttgaatc aaaagacata g
//
LOCUS       DAZKHC020000067        24430 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZKHC020000067 DAZKHC020000000
VERSION     DAZKHC020000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.067"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..544
                     /locus_tag="ACHOSM_RS15610"
                     /old_locus_tag="pgaptmp_003122"
     CDS             <1..544
                     /locus_tag="ACHOSM_RS15610"
                     /old_locus_tag="pgaptmp_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HGU4559597.1"
                     /translation="QGRLNKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCD
                     VPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIR
                     NHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKM
                     RKAAMDRNYLASVLAESGLS"
     gene            complement(586..1356)
                     /gene="yafV"
                     /locus_tag="ACHOSM_RS15615"
                     /old_locus_tag="pgaptmp_003123"
     CDS             complement(586..1356)
                     /gene="yafV"
                     /locus_tag="ACHOSM_RS15615"
                     /old_locus_tag="pgaptmp_003123"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HGU4559598.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            1537..1983
                     /gene="ivy"
                     /locus_tag="ACHOSM_RS15620"
                     /old_locus_tag="pgaptmp_003124"
     CDS             1537..1983
                     /gene="ivy"
                     /locus_tag="ACHOSM_RS15620"
                     /old_locus_tag="pgaptmp_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HGU4559599.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2026..4470)
                     /gene="fadE"
                     /locus_tag="ACHOSM_RS15625"
                     /old_locus_tag="pgaptmp_003125"
     CDS             complement(2026..4470)
                     /gene="fadE"
                     /locus_tag="ACHOSM_RS15625"
                     /old_locus_tag="pgaptmp_003125"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HGU4559600.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4710..5288
                     /gene="lpcA"
                     /locus_tag="ACHOSM_RS15630"
                     /old_locus_tag="pgaptmp_003126"
     CDS             4710..5288
                     /gene="lpcA"
                     /locus_tag="ACHOSM_RS15630"
                     /old_locus_tag="pgaptmp_003126"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HGU4559601.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5494..6261
                     /gene="yafJ"
                     /locus_tag="ACHOSM_RS15635"
                     /old_locus_tag="pgaptmp_003127"
     CDS             5494..6261
                     /gene="yafJ"
                     /locus_tag="ACHOSM_RS15635"
                     /old_locus_tag="pgaptmp_003127"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HGU4559602.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKLLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6232..6972)
                     /gene="dpaA"
                     /locus_tag="ACHOSM_RS15640"
                     /old_locus_tag="pgaptmp_003128"
     CDS             complement(6232..6972)
                     /gene="dpaA"
                     /locus_tag="ACHOSM_RS15640"
                     /old_locus_tag="pgaptmp_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HGU4559603.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPLLASNYTLPEAK"
     gene            complement(7128..7406)
                     /gene="yafQ"
                     /locus_tag="ACHOSM_RS15645"
                     /old_locus_tag="pgaptmp_003129"
     CDS             complement(7128..7406)
                     /gene="yafQ"
                     /locus_tag="ACHOSM_RS15645"
                     /old_locus_tag="pgaptmp_003129"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HGU4559604.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(7409..7669)
                     /gene="dinJ"
                     /locus_tag="ACHOSM_RS15650"
                     /old_locus_tag="pgaptmp_003130"
     CDS             complement(7409..7669)
                     /gene="dinJ"
                     /locus_tag="ACHOSM_RS15650"
                     /old_locus_tag="pgaptmp_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HGU4559605.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            7855..8628
                     /gene="yafL"
                     /locus_tag="ACHOSM_RS15655"
                     /old_locus_tag="pgaptmp_003131"
     CDS             7855..8628
                     /gene="yafL"
                     /locus_tag="ACHOSM_RS15655"
                     /old_locus_tag="pgaptmp_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4559606.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLFCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKHHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            complement(8685..9041)
                     /locus_tag="ACHOSM_RS15660"
                     /old_locus_tag="pgaptmp_003132"
     CDS             complement(8685..9041)
                     /locus_tag="ACHOSM_RS15660"
                     /old_locus_tag="pgaptmp_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGU4559607.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(9034..9312)
                     /locus_tag="ACHOSM_RS15665"
                     /old_locus_tag="pgaptmp_003133"
     CDS             complement(9034..9312)
                     /locus_tag="ACHOSM_RS15665"
                     /old_locus_tag="pgaptmp_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGU4559608.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            complement(9417..10757)
                     /locus_tag="ACHOSM_RS15670"
                     /old_locus_tag="pgaptmp_003134"
                     /pseudo
     CDS             complement(9417..10757)
                     /locus_tag="ACHOSM_RS15670"
                     /old_locus_tag="pgaptmp_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            10729..11514
                     /gene="lafU"
                     /locus_tag="ACHOSM_RS15675"
                     /old_locus_tag="pgaptmp_003135"
     CDS             10729..11514
                     /gene="lafU"
                     /locus_tag="ACHOSM_RS15675"
                     /old_locus_tag="pgaptmp_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HGU4559609.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            11585..12640
                     /gene="dinB"
                     /locus_tag="ACHOSM_RS15680"
                     /old_locus_tag="pgaptmp_003136"
     CDS             11585..12640
                     /gene="dinB"
                     /locus_tag="ACHOSM_RS15680"
                     /old_locus_tag="pgaptmp_003136"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGU4559610.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            12637..13089
                     /gene="yafP"
                     /locus_tag="ACHOSM_RS15685"
                     /old_locus_tag="pgaptmp_003137"
     CDS             12637..13089
                     /gene="yafP"
                     /locus_tag="ACHOSM_RS15685"
                     /old_locus_tag="pgaptmp_003137"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4559611.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKPVGFITCVEHYIDMLFVDPEYTRREVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            13335..14473
                     /locus_tag="ACHOSM_RS15690"
                     /old_locus_tag="pgaptmp_003138"
                     /pseudo
     CDS             13335..14473
                     /locus_tag="ACHOSM_RS15690"
                     /old_locus_tag="pgaptmp_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            14470..15084
                     /gene="prfH"
                     /locus_tag="ACHOSM_RS15695"
                     /old_locus_tag="pgaptmp_003139"
     CDS             14470..15084
                     /gene="prfH"
                     /locus_tag="ACHOSM_RS15695"
                     /old_locus_tag="pgaptmp_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HGU4559612.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLEIET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(15141..16598)
                     /gene="pepD"
                     /locus_tag="ACHOSM_RS15700"
                     /old_locus_tag="pgaptmp_003140"
     CDS             complement(15141..16598)
                     /gene="pepD"
                     /locus_tag="ACHOSM_RS15700"
                     /old_locus_tag="pgaptmp_003140"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU4559613.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            16859..17317
                     /gene="gpt"
                     /locus_tag="ACHOSM_RS15705"
                     /old_locus_tag="pgaptmp_003141"
     CDS             16859..17317
                     /gene="gpt"
                     /locus_tag="ACHOSM_RS15705"
                     /old_locus_tag="pgaptmp_003141"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU4559614.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            17409..18674
                     /gene="frsA"
                     /locus_tag="ACHOSM_RS15710"
                     /old_locus_tag="pgaptmp_003142"
     CDS             17409..18674
                     /gene="frsA"
                     /locus_tag="ACHOSM_RS15710"
                     /old_locus_tag="pgaptmp_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU4559615.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYY
                     SLYERYFAPRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAA
                     FGFRFGANVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGM
                     HDASDEALRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGK
                     LLEIPFNPVYRNFDKGLQEITGWIEKRLC"
     gene            18732..19133
                     /gene="crl"
                     /locus_tag="ACHOSM_RS15715"
                     /old_locus_tag="pgaptmp_003143"
     CDS             18732..19133
                     /gene="crl"
                     /locus_tag="ACHOSM_RS15715"
                     /old_locus_tag="pgaptmp_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU4559616.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVIERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(19172..20227)
                     /gene="phoE"
                     /locus_tag="ACHOSM_RS15720"
                     /old_locus_tag="pgaptmp_003144"
     CDS             complement(19172..20227)
                     /gene="phoE"
                     /locus_tag="ACHOSM_RS15720"
                     /old_locus_tag="pgaptmp_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGU4559617.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            20515..21618
                     /gene="proB"
                     /locus_tag="ACHOSM_RS15725"
                     /old_locus_tag="pgaptmp_003145"
     CDS             20515..21618
                     /gene="proB"
                     /locus_tag="ACHOSM_RS15725"
                     /old_locus_tag="pgaptmp_003145"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGU4559618.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            21630..22883
                     /gene="proA"
                     /locus_tag="ACHOSM_RS15730"
                     /old_locus_tag="pgaptmp_003146"
     CDS             21630..22883
                     /gene="proA"
                     /locus_tag="ACHOSM_RS15730"
                     /old_locus_tag="pgaptmp_003146"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGU4559619.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAVTVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            22998..23073
                     /locus_tag="ACHOSM_RS15735"
                     /old_locus_tag="pgaptmp_003147"
     tRNA            22998..23073
                     /locus_tag="ACHOSM_RS15735"
                     /old_locus_tag="pgaptmp_003147"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23031..23033,aa:Thr,seq:cgt)
     gene            complement(23088..24206)
                     /locus_tag="ACHOSM_RS15740"
                     /old_locus_tag="pgaptmp_003148"
     CDS             complement(23088..24206)
                     /locus_tag="ACHOSM_RS15740"
                     /old_locus_tag="pgaptmp_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4559620.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKMTNRRHEE
                     NWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKV
                     PQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRV
                     AWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKM
                     RYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRY
                     AHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
     gene            complement(24128..>24430)
                     /locus_tag="ACHOSM_RS15745"
                     /old_locus_tag="pgaptmp_003149"
     CDS             complement(24128..>24430)
                     /locus_tag="ACHOSM_RS15745"
                     /old_locus_tag="pgaptmp_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4559621.1"
                     /translation="ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQACIAALQSPL
                     HTVQVSAGDGITEERKCHSSAEVKYGTPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
BASE COUNT         5938 a         6212 c         6231 g         6049 t
ORIGIN      
        1 ccaggggcgg cttaataaag cctttgagga aaaatttccg ctgaaagaat taaataatcc
       61 agagcatgac agttacgcaa tcagtgaaaa gagtcacggc agagaagaaa tccgtcttca
      121 tattgtttgc gatgtccctg atgaacttat tgatttcacg tttgaatgga aagggctgaa
      181 gaaattatgc gtggcagtct cctttcggtc cataatagca gaacaaaaga aagagccaga
      241 aatgacggtc agatattata tcagttctgc tgatttaacc gcagagaagt tcgccacagc
      301 aatccgaaac cactggcacg tggagaataa gctgcactgg cgtctggacg tggtaatgaa
      361 tgaagacgac tgcaaaataa gaagaggaaa tgcagcagaa ttattttcag ggatacggca
      421 catcgctatt aatattttga cgaatgataa ggtattcaag gcagggttaa gacgtaagat
      481 gcgaaaagca gctatggaca gaaactatct ggcgtcagtc cttgcggaga gcgggctttc
      541 gtagtcttac cccgactctc ccccagcctt aaacacaacc cccactcacc gcaacctaaa
      601 ctcatccgca tcctgccatg ccggaaactt ttctctatat tcccgcaatg ccatcatcga
      661 cagctccgca tcaatgcgcg ttgcctgatg cgcgtcggca gtagcgataa tctcgccttg
      721 tggattaatc acccggctgt caccgcgata atggcagccg ttgccatcgc tgccgacgcg
      781 attgcatccc gccacatacg cctgattctc aatcgcgcgg gccgtcagca atgcctgcca
      841 gtgcagagag cgcggagcag gccagttggc aacgtagatg gcgaggtcat aatcgttgag
      901 attgcgcgac cacaccggaa aacgtaagtc gtagcacacc aacggcaaaa tacgccagcc
      961 gcgccattcc acaatcactc gcgcattgcc cgctttataa tgtagatgct catctgccat
     1021 gcggaacaga tgacgcttat cataaaaatg taccgtgccg cccggctcaa ccagcaaaaa
     1081 gcggttaacc gaaccagact ccgtttgtaa tgcaacactg cctgcaatca gcgcattgca
     1141 ctgctgcgcc ttagctgtca tccagttcac tacgtcatct tgtgctagcg acgaagctgc
     1201 cgcttccatg gcaaagccgc tggtaaacat ctccggtaga acgatcacat cgcgcccggt
     1261 aataccttcc agttgacgat caaaatgacg caggttggca gggccatcca tccacaccag
     1321 tggttgctgc aaaagcgtaa tcttcaaacc aggcacggtg tacaactcct ttatgcgaag
     1381 ggttttataa ctttaacacc ttatcaggca gttgccttag cgcagaataa attgataaca
     1441 aatgctgata ttggaaatat ctgatttgca aattatcgtg ttatcgccag gctttagaag
     1501 gttaataaca tgggcaggat aagctcggga ggaatgatgt ttaaggcaat aacgacagtc
     1561 gccgctctgg tcatcgccac cagtgcaatg gcgcaggatg atttaaccat tagcagcctt
     1621 gcaaagggcg aaaccaccaa agctgcattt aatcagatgg tacaagggca taagctgcct
     1681 gcctgggtga tgaaaggcgg tacttatact cccgcacaaa ccgtaacgtt gggagatgag
     1741 acgtatcagg tgatgagcgc gtgcaaaccg catgactgtg gctcgcaacg tatcgctgtg
     1801 atgtggtccg agaaatctaa tcagatgacg gggctgttct cgactattga tgagaaaacg
     1861 tcgcaagaga aactcacctg gctgaatgtg aacgatgcgc tttcgattga tggtaaaacg
     1921 gtgctgttcg cggcgttgac cggcagcctg gaaaaccatc cggatggctt taattttaaa
     1981 taattagcgg ataaagaaac ggagcctttc ggctccgtta ttcaattacg cggcttcaac
     2041 tttccgcact ttctccggca actttaccgg cttcgtcgcc agctcttccg gatcaaagtc
     2101 atcaacgtta atactgcgca gacggctttc ttcagctttc accagaatag cggcttcatc
     2161 tttatcaatc agccccttcg ccagcgcgtt gtgcgccagt tcatccagac gggtaaacgg
     2221 caggttttta cccagctctt tacagatccg ctgatgaatt gggtcggcgg caatcacatc
     2281 caccagcgcc tcttccagca agccaaccgg attatgctcg ctcggcgtca ggtactgacc
     2341 gcgaccaatg cgggaacggg tggcgttcgg cacttgtaaa atcttcgcca ctttatgatc
     2401 cagcttgtca gaaggtgcca gataatgacg tccggtcggg aagatcacca cattcagcag
     2461 cccggcaacc acgcggttcg ggaagttttg cagtaaatca tccatcgcct gttcagcctg
     2521 atacagcgca tcttgtacgc cccagtgcac cagcggcagg tcggcttcat tacggccttc
     2581 gtcgtcataa cgcttcagca cggcagaggc gaggtagagc tggcttaaaa tatcccccag
     2641 acgggccgag atgcgctcgc gacgtttcag gctgccgccc agcactgcca tcgagacatc
     2701 agaaagcagg gcgaggttgg cgctcaggcg gttcaggtgc tgatagtagc gtttagtggc
     2761 atcgccggtt ggcgtgctgc tggttaaacc gcgcgtcagg cccagccaga agctgcgaac
     2821 tttgttgcta ccgacgtgac cgatatgttt gaacaacagt ttatcgaacg cgttgacgtc
     2881 attgttcttc gccgcttcca tctcttccag cacgtacgga tggcaacgaa tcgctccttg
     2941 tccgaagatc atcatgctgc gggtcagaat gttagcccct tcaacggtga tggcaatcgg
     3001 tgcgccctgg taagcacgcg ccaggaagtt gctttgcccg agcataatgc ctttgccacc
     3061 agtgatatcc atcgcatcaa taatcgactg ctgcccgcgg tgggtacagt gatacttaac
     3121 gatagccgac agcacggcag gtttttcgcc gagcataatg ccgtaggtaa tcagcgatgc
     3181 cgcagcatcc atcacgtagg cattaccggc aatacgcgcc agcggctctt caatcccttc
     3241 catcttaccg atagagattt tgaactgacg gcgaatgtga gcatacgcgc ccgttgccag
     3301 cgctaccgat ttcacgccgc cggttgagtt ggaaggcagg gtgatgccgc ggcctaccga
     3361 gaggcactcc accagcatcc gccagccttg cccggccatt ttcggcccac cgatgatgta
     3421 atcgatcggt acgaagacat ctttaccgcg cgtcggtccg ttctggaacg gtacgttcag
     3481 cgggaagtgg cgacgaccaa tttccacgcc cggcgtggtg gtcgggatca gcgcacaggt
     3541 aatacctaaa tcttctgcgc cacccagcag cttttccggg tcggagagtt taaacgccag
     3601 cccaagcaca gtcgcaatcg gtgccagcgt aatatagcgt ttgttccagg tcagacgcat
     3661 ccccagcacc tgctggccct gccattcgcc catgcagaca atcccggtgt ccggaatcgc
     3721 gcccgcatcg gaacccgctt ccgggcttgt cagtgcaaag caagggatct cctgaccacg
     3781 cgccagacgc ggcagatagt gatctttctg ctcgtcagtg ccgtaatgtt gcagcagctc
     3841 gcccgggcct aatgagttcg gcacgccgac ggtaatcgcc aggatcccgc tcacgccgga
     3901 gagtttttgc agcacgcgag actgggcata agctgagaac tccagtccgc cgtactcttt
     3961 tttgataatc atcgcgaaga agcgatgctc tttaaggtac gcccacaact ccggcggcag
     4021 atccgccagc tcatgagtaa tctggaaatc attcgccatc cggcaggctt cttctaccgg
     4081 gccgtcgaga aacgcttgct cttcggcggt caggcgcggc tgcggatagt tatgcagctt
     4141 tttccagtcc ggcttgccct ggaacaagtc gccctcccac caggtggtgc ccgcatcaat
     4201 cgcttctttc tcagtgcgcg acatcggcgg catcacctta cggaaaccgc gaaataccgg
     4261 cgcggaaatc atcgacttac gcataggcgc aaagttaaat ggcacgagga taatggccag
     4321 aggcaccagt acccacgccg accacagacc agcaacgccg agggcggctg tccaggcgag
     4381 caaaatcaga ctgctgataa ataagctcac gcggtgatag aacaacgcgc cgagcaggac
     4441 aaccgtagcg agaatactca aaatcatcat aacgaaaagc cccttacttg taggaggtct
     4501 gaccacttgt gatgatatgg ttgtagtgga tgtaaaaaca tttagcaatg tgtttacaat
     4561 ataattacaa caaagctcac attgttgctg tttttatccg cacttcaggt caaaaagtcc
     4621 tggtcatagc acctgcccgt acttctcgct tttggcggta tccggtacac tgcattttgt
     4681 ctattacatt tatgctgaag gatatcctca tgtaccagga tcttattcgt aacgaactga
     4741 acgaagcggc ggaaacgctg gctaactttt taaaagatga cgccaatatt cacgccattc
     4801 agcgcgcggc ggtcctgtta gcagacagct ttaaagccgg tggcaaagtg ctttcctgcg
     4861 gcaacggcgg ttcccattgc gacgctatgc actttgccga agagttgacc ggtcgctacc
     4921 gtgaaaaccg tccgggctac ccggcgattg ctatttctga cgttagtcat atttcctgcg
     4981 tcggtaatga tttcggtttc aatgatattt tctcccgcta cgttgaagcg gtaggtcgcg
     5041 aaggcgatgt actgctgggg atctccacct ccggtaactc tgcaaacgtg atcaaagcga
     5101 tcgcagcggc gcgtgagaag gggatgaaag tgatcaccct gaccggtaaa gacggcggca
     5161 aaatggctgg cacggcggat atcgaaattc gcgtaccgca ctttggttat gccgaccgca
     5221 ttcaggaaat tcacattaaa gtgatccata tcctgatcca gttaattgaa aaagagatgg
     5281 ttaagtaagt ctggcgtagg ccggataagg cgtttacgcc gcatccggca cttgtgcgct
     5341 gatgcctgat gcgatgctga cgcgtcttat catgcctaca aatctgtacc cgaaccgtag
     5401 gccgaataat tcgtttacgc cgcatccgac ctgaaaattc ttaaatcaat cttcgccggg
     5461 ggccatgcgc tcccgctgtt gtggaggtta cccatgtgcg aattgctcgg gatgagcgcc
     5521 aacgtcccta ccgatatctg ctttagtttc accggacttg tacagcgtgg tggtggaacc
     5581 gggccacata aagatggctg gggcattacc ttttacgaag gtaaaggctg tcgcacattt
     5641 aaagatccac aacccagctt taattccccc atcgccaaac ttgtccagga ctacccgata
     5701 aaatcctgtt cggtggtggc tcatattcgc caggctaatc ggggcgaggt ggcgctggaa
     5761 aatactcacc catttacccg cgagttatgg gggcgtaact ggacttatgc ccataacgga
     5821 caactgacgg gctacaaatt actggaaacc ggcaacttcc gcccggtagg cgaaaccgac
     5881 agcgaaaaag ccttctgctg gctgctgcat aaattaacgc agcgttaccc gcgtacaccg
     5941 ggcaacatgg cggcagtgtt taaatatatc gcctcactgg cggatgaact gcggcagaag
     6001 ggcgttttca atatgctgct ttctgacggg cgctatgtaa tggcgtattg ctcgactaat
     6061 ttacactgga tcacccgccg cgcgccgttt ggcgtggcaa cgttgctgga tcaggatgtg
     6121 gaaatcgact tcagctcgca gaccacaccg aatgatgtgg tcacggtgat tgcaacacag
     6181 ccgctgacgg gcaatgaaac ctggcaaaag attatgccag gcgaatggcg cttattttgc
     6241 ctcggggagc gtgtagtttg atgccagtag tggctgcaca acttcgtggc ttaacggctt
     6301 gctgaccacg taacggccat tgaccacaga aacggtcggt ggcttacggg tttgctcaaa
     6361 gtagtcgtag cccggtttca gttgctccca gaagtcctta aagttggaat acttatggcg
     6421 cttcatattg gcgtcggtca tgcggaacgg gtaaatactc acttgcacgc tcggctgacc
     6481 aaacaccagc gcaccagtaa cgaactggaa gatctcatca ataccctgat tggtcattgc
     6541 gtagcagccg atggaaacac aatcgccgtg gatcatcaga tatttccctt cataaccatg
     6601 cgcacggtca taggcattgg ggaaaccaat attaatcgct ttgtagtaac ggctgtctgg
     6661 ttttaactga ttacgctgga cgctataaaa cccttccggg cttttgaaat caccctgacg
     6721 ctgttttggc cctaatccgc cggaatattt acagatttta taactgtcga gcagctgata
     6781 ttgctcgccc attttgacgt agagatcgag cgtacgttct tccttgaaga tctggatgta
     6841 gacaggggat cccatcaact gctgcttata ctctttgctc actggcgtgg tcgagctact
     6901 gctgcccagc aaaccggcaa acgaaacgca cgggatcaac aacatcgcaa gaattaatgc
     6961 gattttacgc atactgctta ttccttgata aaacggttac acacgccaag acggcaaaat
     7021 ggatcccaaa tcggaatagt ctggatttgg aaggctcaca ttatcaccaa aagagtttta
     7081 cgcaagcctg tcggcgcagg gtttacaaat ttcatcggaa gcgggcttta cccaaagagc
     7141 gccgcgtgag ttccagttct ctcaaatcgt aaaagtttat cggtaagttt gtaaatcagg
     7201 atccagtccg gttcgacatg agcatcgcga taacctttcc atgaaccttg cagcgggtgg
     7261 tctttataaa cagctggaag cggtaaagta ttattgataa gaagcgtcat aagatatttc
     7321 aatttattca tatccttatg acgcttttgt gcaagtttta catcctttga aaattgtccc
     7381 gagtattcaa tatccctttg aatcatattt aaattcctaa tttatcaaat aaatcatcgg
     7441 cgtctttggc cttatgaaca tcaacgccag cttcgctgtt tttgattgat tgaatggtta
     7501 attgattagg ctcgcgtaaa tcaaacggca atgccttttc acgcgcgacc tttgtgaggg
     7561 ttatgcgaac caggtcagag atggtcagcc ccatcccggc cagtacgtcc gccgcctgat
     7621 tcttcagatc ttcatcgatt cgggcgcgaa caaatgcgtt agcagccatt ggggtatctc
     7681 ctgtgctgta tatttattca gcttgaattg tagctcaaat gagcaacagc gacaatccat
     7741 gagtaattta ataaacggat gtttttacat aattaactga aatatattta ttaattttat
     7801 aataatggtt tattaagttt gttcaggtac gggccgggat acactgcaac atcaatgcat
     7861 gatcagccat tattgagtat gtccttgccg tcgattccct cttttgtgtt gtcgggatta
     7921 ctgttgtttt gcttgccgtt ttcttcattt gccagcgcca ccacatcaca tatcactttc
     7981 agctacgccg cccgccagcg gatgcaaaat cgtgcgcgtt tattaaaaca gtaccaaacc
     8041 catctgaaaa aacaggccag ttatattgtg gaaggcaatg ccgaaagcag aagggcgcta
     8101 cgccagcaca accgcgaaca gataaaacat catccagaat ggtttcctgc gccgctcaag
     8161 gcgagtgaca gacgctggca ggcgctggcg gaaaacaacc atttcttaag cagcgaccat
     8221 ctgcataaca tcaccgaagt agcgattcac cgtctggagc aacagcttgg caagccttac
     8281 gtctggggcg gtacgcggcc cgatcaaggc ttcgactgta gcgggttagt tttttatgcc
     8341 tacaacaaga tccttgaggc gaaactccca cgcacggcta atgagatgta tcactatcgc
     8401 cgggcaacca ttgtggcgaa taacgacctg cgccggggag atttgctatt tttccatatc
     8461 cacagccgcg agattgccga ccatatgggg gtgtatctgg gcgacggtca gtttattgag
     8521 tcaccgcgca ccggggaaac cattcgggta agtcggttag ctgaaccttt ctggcaggat
     8581 cactttttag gcgcgcggcg gattttgacg gaggagacga ttttgtaaat gggtaatatt
     8641 gacgaactgg caaaaggtcg gatgccacgc ggcatccgac ctgtttacat ctgggctgaa
     8701 aactcacgtt ctaagacatc aacaatgagc tggttttttg aaatggaatg ttgtctggcg
     8761 acgatggtca gccgcgaatc aatgtgatga ggaacacgaa gcgtcaggcg tttttgctct
     8821 tcttcttcac gaaaaggggc aataccgtcc tcttcacaat ccgccatcca ctcatccagc
     8881 gcgctttcgc cttcacgata taatgcctca atacttgtgg ctacaaagtc acaataaccg
     8941 tttacattga ggaacttgcc gcgaaatgcg ttgatatcgg cttcaaatgt cactgcggct
     9001 ggttgacctt taattgtgat aatgttcgat gctttattca taatctaccc ctacacagtt
     9061 aacaaaccac tccttaagac ctgctactgc gcctttatcc atcacattct ttggatgtgg
     9121 ctcatgtgtg ctgaatttgg tcgttccaat ctgaaatttt cgtctggagc cgttgttatt
     9181 ttttattgtt ccacccacag ctttaatgag tgcaacaaca tcatcccatt taattccaga
     9241 ttttggcggc tccgtcatta catcgtcgaa tgtttttcta tgacgagaac ctagcgcgct
     9301 gaccttaatc atactatcaa tccatgacgt cattgtatgg tgtcatcgta gtctctttgc
     9361 agtgtgacgt caattaatgg tgtcaggtgc tgcaaagcat ggaaaagaat cccccatcaa
     9421 ctcaacgccc ccataatctt caactccagc tcgtccggca cttcgttata cgacagcaca
     9481 tgcagccccg gcgcaaacaa ccttgcataa cgcgccagca aagggcgcag ctgcggtggc
     9541 accagcagca ccgggtcttt ccccgccgct ttcatctgct ctttcacctg tggcatcgtg
     9601 ctctggaact ggttgagcat attcgggtcc actggcacgc tgtcgagcat cactttaccg
     9661 ccctgttgcg cctgattcac taggttagtc agcagatttt ccagctcatt attcagggta
     9721 tacaccgtca gctcctgctt gcgaacgaac ggatgggtaa tgctgcgccg cagcgccagg
     9781 cgcacatcgg ctgccagcag aatatgatct ttggtcaccg cgctactggc gaccagcacg
     9841 gtggcgatgg tgacgatatc gcgcagggaa acgccttcgg tcagcagcgc ccggtacact
     9901 ttcagcaact ggctgtaatt gagcaccgcg cttaaatcct ccgccaggcg cggtgccatc
     9961 gacgacaaac ggttatgcaa ctgcgtaata tcgtcatagt taaataaatc aggaatatag
    10021 ctgcgcacaa tcttatttac atgggtagca atcacgctgg cgctgtcgat cacctgatac
    10081 cccatattca gcgccttcgc tttctgcgca ggctggatcc aggtcaccgg catcccgtac
    10141 gccgggtcgt tccccagcac gccgtctatc tcgccgtagg tttcgctgga gggcagcgcc
    10201 atcagtttat ccgccggaat atccgcttca tcagccttaa tgccgttgat gaaaatagcg
    10261 tactggctgg gcttaaggcg gaagttttcc cgaatgcgga tctccggcag cagcacgccg
    10321 ttgccgtcgg atatcacctg tcgcacgccg cgaatacgct gggtgagcgg gttgccctgg
    10381 gctttgtcca ccagcgccac cagcccgtcg ccgatggtca tcagcacata ctgctggaag
    10441 gcggcgtcgg cgctcaggtt gtatttgaag atcccgatac agacgccgcc gatcaggttg
    10501 atcgccagaa tcatcatccc ggcgatagca tccccgcgca caaatttcga tgccccgtcc
    10561 atcgcgccgt agaaatcggc ctcgctggca acatctttac gtcgggtttg tgcctgcgcc
    10621 tgattgatca acccggcatt aagatcggcg tcaatcgcca tctgcttacc gggcatcgcg
    10681 tccagggtaa agcgggcaga aacctcggat atacgctcgg cccctttggt gacgacaata
    10741 aagttgattg tcaacagcgt cagtaaatcc gagcgtgaaa gcattatcgc cgcgctgcac
    10801 gggcagtcga tttttaacgg tggtgggctg tcgccgttaa ataaaatcag cccgtctcat
    10861 ccgccaaaac ctgccaccgt ggcggtgccg gaagaaacgg agaaaaaagc gcgcgacgtc
    10921 aatgagaaaa cggcgctgct gaagaagaaa tccgccaccg agcttggcga gctggcaacc
    10981 agtatcacca ccattgcccg tgatgcgcat atggaagcga atctggagat ggagattgtt
    11041 ccccagggat tacgcgtgct gattaaagac gaccagaatc gcaatatgtt cgaacgcggc
    11101 agcgcgcaga ttatgccgtt ctttaaaacg ctgctggtgg agctcgcgcc agttttcgac
    11161 tcgctcgata acaaaattat tattaccggg cataccgatg cgatggctta caaaaacaat
    11221 atctacaaca actggaacct ttcgggtgac cgcgcgcttt cggctcgtcg ggtgctggaa
    11281 gaggccggaa tgccggaaga taaagtgatg caggtaagcg caatggcgga ccagatgctg
    11341 ctggattcca aaaatccgca aagcgcgggc aaccggcgca ttgagattat ggtgctgacc
    11401 aaaagtgcgt ccgatacgct gtatcaatac tttggtcagc atggggataa agtggtgcag
    11461 ccgctggtgc aaaagctgga taagcagcag gtgctttcgc agcgaacgcg ttaaatgctg
    11521 aatctttacg catttctcaa actctgaaat cactgtatac tttaccagtg ttgagaggtg
    11581 agcaatgcgt aaaatcattc atgtggatat ggactgcttt ttcgcagcgg tggagatgcg
    11641 cgacaatccc gccctgcgcg atatccctat tgctattggc ggcagccgcg aacgtcgggg
    11701 ggtgatcagt accgccaatt atcccgcgcg taaatttggc gtacgtagcg ctatgccgac
    11761 agggatggcg ctcaaattat gcccgcatct caccttgctt ccggggcgct ttgacgccta
    11821 caaagaagcc tcaaatcata tccgcgaaat cttctcgcgc tacacctcgc gtattgaacc
    11881 gttgtcactg gatgaggctt atctcgacgt caccgatagc gtccattgcc acggttctgc
    11941 gaccctcatc gcccaggaaa tccgccagac gattttcaac gagctgcaac tgacggcgtc
    12001 tgcgggcgtg gcacccgtaa agtttctcgc caaaatcgcc tccgacatga ataaacccaa
    12061 cggccagttt gtgattacgc cggcagaagt tccggcattt ttacaaacct taccactggc
    12121 aaaaatcccc ggcgtcggca aagtctcggc ggcaaaactg gaagcgatgg ggctacgaac
    12181 ctgcggtgat gtacaaaagt gtgatctggt gatgctgctt aaacgctttg gcaaatttgg
    12241 ccgcattttg tgggagcgta gtcaggggat tgacgagcgc gacgttaaca gcgaacggtt
    12301 gcgaaaatcc gtcggcgtgg aacgcacgat ggcggaagat atccaccact ggtctgaatg
    12361 tgaagcgatt atcgagcggc tgtatccgga acttgaacgc cgtctggcaa aggtgaaacc
    12421 tgatttactg attgctcgcc agggggtgaa attaaagttt gatgattttc agcaaaccac
    12481 tcaggagcac gtctggccgc ggctgaataa agctgactta atcgccaccg cgcgtaaaac
    12541 ctgggatgaa cgccgcggcg ggcgcggtgt gcggctggtg gggctgcatg tgacgttgct
    12601 tgacccgcaa atggaaagac aactggtgct gggattatga ataacataca aataagagac
    12661 tatcagcctg gcgattttca gcaactatgc gctattttca ttagagcggt tacgatgact
    12721 gccagtcagc attattcacc acaacaaatt gccgcctggg cgcagattga cgaatctcgc
    12781 tggaaggaga aactcgcgaa atcacaagtg cgggttgcag tcattaatgc aaaaccggtc
    12841 ggctttatta cctgcgttga acactatatc gatatgttat ttgttgaccc tgaatacacc
    12901 cgccgtgagg ttgccagcgc tttgttaaaa cctttgatta agtctgaatc cgaacttacg
    12961 gtggacgcaa gcataaccgc aaaacccttt tttgaacgtt atggttttca gacagttaag
    13021 cagcaacgcg ttgaatgtcg gggagagtgg tttattaatt tttatatgcg ttataaacag
    13081 caacattaaa tccagcttgc aatgaaaata acgcaagcct agtatgtgcg cgttttctta
    13141 ttccacaaac tgcaaggagg taaatcatga caaatccttt atcaatgact ctttgcagac
    13201 ctttccagga ttaattcttt ttttcttgcc ctggattcgt ctgccatttc ctgatttttt
    13261 aattgttact ctcaatactt caagtcgcaa cacatgcaac ttgaagtatg acgagtctat
    13321 ttacggaatg gtatatgggc aattatattc gtcccttatc cgatgcggta tttaccatcg
    13381 catctgatga cctgtggatc gagagtttag cgatccaaca attacacacc acggcaaatt
    13441 tacccaacat gcagcgcgta gtggggatgc cagatttaca tcccggacgc ggctatccga
    13501 ttggcgcagc gttcttctct gttggtcgtt tttatccggc actggtcggc aatgatatcg
    13561 gctgcggtat ggcgctatgg caaacagata ttctcgctcg caaatacaac gccgataagt
    13621 ttgaaaagcg attatcagat ctggatgacg ttgctgaaga aagctggctg gagaaaaacc
    13681 tgccgtcagc atttgcacag catccgtggc gcagctcgct tggctctatc ggtggcggca
    13741 atcatttcgc tgaactgcaa caagttgatc aaatcatcaa cgctgaactg tttgcactgg
    13801 caggtctgga tgcacaacat ctgcaactgt tggttcatag cggctcgcgg ggggggggca
    13861 gtctatttta cagcggcata ttgcctcgtt ctcgcatcat ggtttgcctc aaggcagtga
    13921 cgacgcgcta cggtatattg cggaacatga cgatgcgctg gcgtttgcgt gtattaatcg
    13981 ccagatgatc gctttgcgca taatgcaaca agttaaggcc acgggaagtc cggttctgga
    14041 tgtggcgcat aactttgtta gcgcgtgtca aatcggcgat caacagggct ggttgcatcg
    14101 taaaggtgcc acgccggatg acaacggtct ggtgattatt cccggttcac gtggtgatta
    14161 ctcctggatt gtacagcctg ttgcgaacga aaaaacgctc cactcgctgg cgcatggggc
    14221 agggcgtaaa tgggggcgca ccgagtgtaa agggcgtctg gcagcgaaat acacggtgac
    14281 gcaactctcg cggactgaac ttggcagtcg ggtaatttgt cgcgataaac aactcatctt
    14341 tgaagaagcg ccacaagcct ataaatcggc tgaaagcgtg gtgcaatgtc tggtgcaggc
    14401 cggtttaatt attcctgtcg cgcggctgcg tccggtgcta acgctcaaaa acagtggagg
    14461 gaaaaaagga tgatcttgct acaactctcc tcagcgcagg ggccggaaga atgttgtctc
    14521 gcagtgaaaa aggcgctgga caggctaatt aaagaagctg cccgacaaga cgtcgcggta
    14581 acggtgttgg aaatagaaac gggccgctac tctgacacgc tgcgttcagc tctggtttct
    14641 ctggatggcg ataacgcatg ggcattaagc gaaagctggt gcggcactat tcagtggatt
    14701 tgtccgagtc cgtatcggcc tcatcatggg cgcaaaaact ggtttctggg cattgggcgt
    14761 tttaccgctg atgagcagga acaatcggat gcaatccgtt atgagacgtt gcgttcgtcg
    14821 gggccgggcg gtcaacatgt caataaaacc gactcggcgg tacgcgccac gcatttggca
    14881 tccggtatta gcgtgaaggt tcagtcagag cgtagtcagc atgctaacaa gcggctggca
    14941 cgattgctga ttgcctggaa gctggagcag cagcaacagg aaaatagcgc ggtgctgaaa
    15001 tcgcagcggc gaatgttcca tcaccagatt gaacgtggca acccgagacg gacatttaca
    15061 gggatggctt ttatcgaagg ataatgaagg atgaaactgc cggaaggcga ttaaacgcca
    15121 tccggcagca aatcaaataa ttacttcgcc ggaatttctt tcagcagttc agtcagcagc
    15181 gtccagtaat gacctacgct ttcgatgtga acttgctcat ccggagagtg tggaccggtg
    15241 atagttggcc cgatagaaac catgtccatt tccggatacg gttttttgaa cagaccacat
    15301 tccaggcccg cgtggataat ctggatgttc ggtgttttgt tgaacagacg ctgataggtt
    15361 tcacgtacca gatgcatcac cggagaatta gcgtccggct gccagccagg atatgcgcct
    15421 ttcgcttcgg ttttcgcgcc agctagttta cccagcgaat ccagcatgct caccacgtag
    15481 tctttaccgc tgtcgatcag tgaacggatc aggcagtgaa tttctacgtt attgtcagtc
    15541 atggtcacca caccgacgtt cagggaggtt tcgaccacac cttttgccac gtcggaattg
    15601 cggatcacac cgttcggggt ggcgttcagc agacgaataa aggtatcgcg agatttcgca
    15661 atcagggcag ctttatcgtt cgctacagag tccagcaaca aggccagatt tttctctttc
    15721 tctgccagct cgtttttcag gatctcctga taggtattca ccagagattt caggacgtcg
    15781 actttatcag ctgcgacagc aatggtcgca aaggcttcac gcgggatggc gttacgcagt
    15841 gtgccgccgt tgaaatcgat aaggcgcaga tccagttctt ccgcatgacc cgccaggaag
    15901 cgcaccagta gtttgttggc attacccagg ccaacgtgga tttcaccgcc ggagtgaccg
    15961 cctttcagac cttttaaggt taacttgaag gtttcaaaac cagctggaac cgcttcacga
    16021 tctaaatgca ggttggaggt gaagtcgata ccccccgcac aacccatgta gatttcacct
    16081 tcttcttcgg agtcggtgtt aatcagaata tcagcctgca accagttgct ctgtaagccg
    16141 aacgcaccgt ccataccggc ttcttcggtc atggtcagca gcacttccag cgggccgtga
    16201 accacgtttt cgtcagccag aactgccagt gcagaggcca taccaatgcc gttatccgca
    16261 cccagcgtgg tgccgcgcgc tttaacccat tcgccatcaa tataaggctg gataggatct
    16321 ttcgtgaagt catgcacggt gtcgttattt ttctgcggca ccatatcgag gtgggcctgt
    16381 aagacgaccg gtttacgatt ttccatacct gcggtagcag gtttacgaat caggatatta
    16441 cctacctgat cgcgttcgac atggaaacct ttctcttttg cccaaccaac aatgtattca
    16501 gcgagttgct cttcatgata ggacgggtga ggaatagaac agattttggc aaaaatatcc
    16561 cacagcggct gtggagataa ttgagacagt tcagacacgt taagtctcct tgtcgatcac
    16621 ccgcaaaaca gtattgcagg tcacagggtt agcagaaaat gttgtcaaca caagacaggc
    16681 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggcc gtgcgtaaaa
    16741 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
    16801 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
    16861 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
    16921 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctggt
    16981 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
    17041 cagctacgat cacgacaacc agcgagagct taaagtgctg aaacgcgcag aaggcgatgg
    17101 cgaaggcttc atagttattg atgacctggt ggataccggt ggtactgctg ttgcgattcg
    17161 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
    17221 ggttgatgac tatgttgttg atattccgca agatacctgg atcgaacagc cgtgggatat
    17281 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
    17341 gggcgttgtt ctttttaact tcaggcaggt tacaatagtt tccagtaagt attctggagg
    17401 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
    17461 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
    17521 ctcttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
    17581 ggcgcggcat tgacccacgt gaaatcctcg acgtccaggc gcgtattgtg atgagcgatg
    17641 ccgaacgtac cgacgatgat ttatacgata ccgtgattgg ctaccgtggt ggcaactgga
    17701 tttatgagtg ggccacccag gcgatggtgt ggcaacaaaa agcctgtgcg gaagaagatc
    17761 cgcaactcag tggtcgacac tggctgcatg cggctacgtt gtacaacatt gccgcctatc
    17821 ctcatctgaa aggagatgac ctggccgagc aagcgcaggc tttatcaaac cgcgcctatg
    17881 aagaggccgc ccagcgtctg ccgggcacga tgcggcagat ggagtttacc gtacccggcg
    17941 gtgcgcccat cacccccggc ggtgcgccca tcaccggctt tttgcatatg ccgaaaggcg
    18001 atggcccgtt cccgacagta ttaatgtgtg gtggtctgga tgcgatgcag acggactatt
    18061 acagcctgta tgaacgttat tttgcgccgc gcggcattgc gatgctgact attgatatgc
    18121 cgtcggtggg cttttcttca aaatggaagc tcacccagga ctccagtctg ttgcatcagc
    18181 acgtcttaaa ggcgctgcct aacgtaccgt gggtggatca cactcgcgtc gcggcctttg
    18241 gtttccgttt cggcgctaac gttgccgtgc gtctggcata ccttgaatcg ccgcgtctga
    18301 aagcggttgc ctgtcttggt ccggtagttc ataccctgtt gagtgatttt aagtgccagc
    18361 aacaggtgcc ggaaatgtat cttgacgttc tggccagtcg tttggggatg catgatgctt
    18421 ccgatgaagc gttgcgcgtg gagctgaatc gctattcatt aaaagtgcaa ggattgctgg
    18481 gacgtcgctg cccaacgcca atgttatcag gctactggaa gaacgatccg ttcagcccgg
    18541 aagaggactc acgcttaatc acctcatcat ctgctgacgg taaattatta gagatcccat
    18601 ttaacccggt gtatcggaat tttgacaaag gtcttcagga aatcaccggc tggatcgaaa
    18661 aacgcttgtg ttaaaaattt gctaaatttt gccaatttgg taaaacagtt gcatcacaac
    18721 aggagatagc aatgacgtta ccgagtggac acccgaagag cagattgatc aaaaaattta
    18781 ccgcactagg cccgtatatt cgtgaaggta agtgcgaaga taatcgattc tttttcgatt
    18841 gtctggctgt atgcgtcaac gtgaaaccgg caccggaagt gcgtgaattc tggggctggt
    18901 ggatggagct ggaagcgcag gaatcccgtt ttacctacag ctaccagttt ggtctgttcg
    18961 ataaagcagg cgactggaag agtgttccgg taaaagatac tgaagtgatt gaacgactgg
    19021 agcacaccct gcgtgagttt cacgagaagc tgcgtgaact gctgacgacg ctgaatctga
    19081 agctggaacc ggcggatgat tttcgtgacg agccggtgaa gttaacggcg tgagtgaaat
    19141 gtgccggatg catcacatcc ggcaataatc attaaaactg ataggtcatg ccaaccgcga
    19201 caatatcatc attattaata ttcaatttgt tatcgctatc cagttggttg attttataat
    19261 caacaaacgc tgacatattt ttgttgaaat aatatgtagc cccgacatcg atataattga
    19321 ccagatcttc atcaccgata ccttcaatat ctttcccttt cgataagaca taacccagcg
    19381 atggacgcag accaaagtca aactggtatt gagcgaccgc ttcaaagttc tgagttttat
    19441 tggcaaagcc gccagatatt ggcgtcattt tgcgtgtttc agaatagaaa gttgccagat
    19501 aaatattatt ggcatcgtat ttcagacctg ttgcccatgc ttctgcacgc ttgcctgtgc
    19561 cacggctttg caggttctgc tcgttggtgc gatctgagtt ggtataggcc ccactaatgg
    19621 cgaaatcgct gccgccaaag tcatatgtca atgacgtgcc gaagccatcg ccgttttgct
    19681 ttttaacgtc gcggttttcg tttttccctt gatattgcag ggttaagttc aggccatcga
    19741 taacgccgaa gaagtcggtg ttccgatatg tcgccagacc gctggcgcgt ttggtcataa
    19801 agttgtcggt ctgcgccgag gagtcgccac caaattccgg gaacatatcg gtccaggctt
    19861 ccacgtcata caacgcgccc aggttacgac cataatcgaa agaacccaaa tctttatatt
    19921 tcaacccggc aaaagcgaga cgcgtttttt gctgtgcagt atcactctct gctttattac
    19981 cggcaaactc tgcttcccaa cgaccataac cagtcagttg atcgttaatt tgtgtttcgc
    20041 ctttaaaacc aaaacggata taactctggt cgccatcttt actgtcgtta tcactcatat
    20101 aatgcatggc tttaactttg ccatagacat ccagtttatt accgtctttg ttatatattt
    20161 ctgcggcctg tacggatgca gatgccacaa tgcccatcac cactaatgcc agagtgctct
    20221 ttttcatttt cattcctgat tttaattaac gcgcgaatat tcagcgggag agccccgttg
    20281 aaaacaggaa agtttttaac ctgagattgt taaagatata ttacagatta ataatattct
    20341 taaaatgtgg taatttatta aatctgtaat aaaagcgtaa acaactgccg ctaggcttgc
    20401 tgatcccgcg caacaaaacg ccatgctttg ctcgcagatg gttggcaacc gacgacagtc
    20461 ctgctaaaac gttcgtttga tatcattttt cctaaaattg aatggcagag aatcatgagt
    20521 gacagccaga cgctggtggt aaaactcggc accagtgtgc taacaggcgg atcgcgccgt
    20581 ctgaaccgtg cccatatcgt tgaacttgtt cgccagtgcg cgcagttaca tgccgccggg
    20641 catcggattg ttattgtgac gtcgggcgcg atcgccgccg gacgtgagca cctgggttac
    20701 ccggaactgc cagcgactat cgcctcgaaa caactgctgg cagcggtagg gcagagtcga
    20761 ctgattcaac tgtgggaaca gctgttttcg atttatggca ttcacgtcgg gcaaatgctg
    20821 ctgacccgtg ctgatatgga agaccgtgaa cgcttcctga acgcccgcga caccctgcgt
    20881 gcgttgctcg ataacaatat cgttccggta atcaatgaga acgatgctgt cgctacggca
    20941 gagattaaag tcggcgataa cgataacctt tctgcgctgg cggcgattct ggcgggtgcc
    21001 gataaactgt tgctgctgac cgatcaaaaa ggtttgtata ccgctgaccc gcgcagcaat
    21061 ccgcaggcag aactgattaa agatgtttac ggcattgatg acgcactgcg cgcgattgcc
    21121 ggtgacagcg tttcaggcct cggaactggc ggcatgagta ccaaattgca ggccgctgac
    21181 gtggcttgcc gtgcgggtat cgacaccatt attgccgcgg gcagcaagcc gggcgttatt
    21241 ggtgatgtga tggaaggcat ttccgtcggt acgctgttcc atgcccaggc gactccgctt
    21301 gaaaaccgta aacgctggat tttcggtgcg ccgccggcgg gtgaaatcac ggtagatgaa
    21361 ggggcaactg ccgccattct ggaacgcggc agctccctgt tgccgaaagg cattaaaagc
    21421 gtgactggca atttctcgcg tggtgaagtc atccgcattt gcaacctcga aggtcgcgat
    21481 atcgcccacg gtgtcagtcg ttacaacagc gatgcattac gccgtattgc cggacaccac
    21541 tcgcaagaaa ttgatgcaat actgggatat gaatacggcc cggttgccgt tcaccgtgat
    21601 gacatgatta cccgttaagg agctggccga tgctggaaca aatgggcatt gccgcgaagc
    21661 aagcctcgta taaattagcg caactctcca gccgcgaaaa aaatcgcgtg ctggaaaaaa
    21721 tcgccgatga actggaagca caaagcgaaa tcatcctcaa cgctaacgcc caggatgttg
    21781 ctgacgcgcg agccaatggc cttagcgaag cgatgcttga ccgtctggca ctgacgcccg
    21841 cacggctgaa aggcattgcc gacgatgtac gtcaggtgtg caacctcgcc gatccggtgg
    21901 ggcaggtaat cgatggcggc gtactggaca gcggcctgcg tcttgagcgt cgtcgcgtac
    21961 cgctgggggt tattggcgtg atttatgaag cgcgcccgaa cgtgacggtt gatgtcgctt
    22021 cgctgtgcct gaaaaccggt aatgcggtga tcctgcgcgg tggcaaagaa acgtgtcgca
    22081 ctaacgctgt aacggtggcg gtgattcagg acgccctgaa atcctgcggc ttaccggcgg
    22141 gtgccgtgca ggcgattgat aatcctgacc gtgcgctggt cagtgaaatg ctgcgtatgg
    22201 ataaatacat cgacatgctg atcccgcgtg gtggcgctgg tttgcataaa ctgtgccgtg
    22261 aacagtcgac aatcccggtg atcacaggtg gtataggcgt atgccatatt tacgttgatg
    22321 aaagtgtaga gatcgctgaa gcattaaaag tgatcgtcaa cgcgaaaact cagcgtccga
    22381 gcacatgtaa tacggttgaa acgttgctgg tgaataaaaa catcgccgat agcttcctgc
    22441 ccgcattaag caaacaaatg gcggaaagcg gcgtgacatt acacgcagat gcagctgcac
    22501 tggcgcagtt gcaggcaggc cctgcgaagg tggttgctgt taaagccgaa gagtatgacg
    22561 atgagtttct gtcattagat ttgaacgtca aaatcgtcag cgatcttgac gatgccatcg
    22621 cccatattcg tgaacacggc acacaacact ccgatgcgat cctgacccgc gatatgcgca
    22681 acgcccagcg ttttgttaac gaagtggatt cgtccgctgt ttacgttaac gcctctacgc
    22741 gttttaccga cggcggccag tttggtctgg gtgcggaagt ggcggtaagc acacaaaaac
    22801 tccacgcgcg tggcccaatg gggctggaag cactgaccac ttacaagtgg atcggcattg
    22861 gtgattacac cattcgtgcg taaataaaac cgggtgatgc aaaagtagcc atttgattca
    22921 caaggccatt gacgcatcgc ccggttagtt ttaaccttgt ccaccgtgat tcacgttcgt
    22981 gaacatgtcc tttcagggcc gatatagctc agttggtaga gcagcgcatt cgtaatgcga
    23041 aggtcgtagg ttcgactcct attatcggca ccattaaaat caataagtta cacatcatta
    23101 gtaccttcct tattttttga ctgggacaaa tttgggaccg atgggttcag gatcgagtct
    23161 atttgccgtg cgtgttcggt aaggtgatta ggtgcaaggt gagcatatcg acgaaccatt
    23221 tcgatagact cccagcctcc catttcctgt aacactgaca acgggactcc ggcttgaacc
    23281 agccaacttg cccaggtgtg tctcaagtcg tgaaatctga aatcatcaat accagcccgt
    23341 ctcagcgccg ctttccaggc tgtgtttgcg tcataccgca tcttccttac tgttggcgct
    23401 ttcgttccgt ctggtttggt acagctttcc ttgtacacaa atacccaacg gtgatgattc
    23461 ccgatttgtt ttttcaatac gcgacatgca gtatcattca gcgcaacgcc aattgcgcgg
    23521 tttgatttac tctcttccgg gtttatccat gccacccggc gctgcatatc tatttgttgc
    23581 cattcaaggt tgatgatgtt cgagcgtctt aagcctgttg ccagtgcaaa ttcaacaaca
    23641 gactttaatg gctccggaca ttcatcaatc agcctttgtg cttcatgggg ctccagccag
    23701 cggatccgtt tattctttgg ttgaggcact ttaataattg gtgccttatc cagcattttc
    23761 cattcacgct ctgcggctct tagtagggcc tttataaatg aaagatgcgt agccttcgtt
    23821 gcaacggacg ctggttttgg cgtgtattct ggaacaggtt tccctttttt tctgcatgct
    23881 tctgccctga gtttccagtt ttcctcatga cgccggttcg tcattttctg cattgctgaa
    23941 taaatttttg attcagtaat gtctcttagt tgcattcctg cgaaatgttg aagccagaat
    24001 ccgatccggc ttttgtcatc gtccagcgat tttttatgtg ctttctcttc aagccacctg
    24061 acacacgctt cctcgaacgt tatatcaggt atttcaccaa gtttgctgac ccgccatgct
    24121 tcagccttta gcttgtcatg gagttctgtc gcctgccttt tgtcctttgt tccaagagac
    24181 tgtttaaatc ttttaccgtt cggcaatgtg aaactggcgt accatatttc acctctgcgg
    24241 aagagtgaca ttttctttcc tctgttatgc catcacccgc gctcacctgg acagtatgca
    24301 gcggagactg aagagccgca atgcaggctt gtcgtgttgt gaggtaagga gatttattct
    24361 tagtgggatc tttgcgtgtt gcctgaagac gccctgtgcg tatccagtta atggcagtcg
    24421 gtctggatat
//
LOCUS       DAZKHC020000068        23460 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZKHC020000068 DAZKHC020000000
VERSION     DAZKHC020000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.068"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            43..1362
                     /gene="digH"
                     /locus_tag="ACHOSM_RS15750"
                     /old_locus_tag="pgaptmp_003150"
     CDS             43..1362
                     /gene="digH"
                     /locus_tag="ACHOSM_RS15750"
                     /old_locus_tag="pgaptmp_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HGU4559622.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIT
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            1734..3116
                     /gene="dosC"
                     /locus_tag="ACHOSM_RS15755"
                     /old_locus_tag="pgaptmp_003151"
     CDS             1734..3116
                     /gene="dosC"
                     /locus_tag="ACHOSM_RS15755"
                     /old_locus_tag="pgaptmp_003151"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HGU4559623.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            3141..5540
                     /gene="dosP"
                     /locus_tag="ACHOSM_RS15760"
                     /old_locus_tag="pgaptmp_003152"
                     /gene_synonym="pdeO"
     CDS             3141..5540
                     /gene="dosP"
                     /locus_tag="ACHOSM_RS15760"
                     /old_locus_tag="pgaptmp_003152"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HGU4559624.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEAQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            5797..6375
                     /gene="ddpX"
                     /locus_tag="ACHOSM_RS15765"
                     /old_locus_tag="pgaptmp_003153"
                     /pseudo
     CDS             5797..6375
                     /gene="ddpX"
                     /locus_tag="ACHOSM_RS15765"
                     /old_locus_tag="pgaptmp_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            6392..7942
                     /gene="ddpA"
                     /locus_tag="ACHOSM_RS15770"
                     /old_locus_tag="pgaptmp_003154"
     CDS             6392..7942
                     /gene="ddpA"
                     /locus_tag="ACHOSM_RS15770"
                     /old_locus_tag="pgaptmp_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4559625.1"
                     /translation="MKRSIPFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            7944..8966
                     /gene="ddpB"
                     /locus_tag="ACHOSM_RS15775"
                     /old_locus_tag="pgaptmp_003155"
     CDS             7944..8966
                     /gene="ddpB"
                     /locus_tag="ACHOSM_RS15775"
                     /old_locus_tag="pgaptmp_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4559626.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            8963..9859
                     /gene="ddpC"
                     /locus_tag="ACHOSM_RS15780"
                     /old_locus_tag="pgaptmp_003156"
     CDS             8963..9859
                     /gene="ddpC"
                     /locus_tag="ACHOSM_RS15780"
                     /old_locus_tag="pgaptmp_003156"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HGU4559627.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            9856..10842
                     /gene="ddpD"
                     /locus_tag="ACHOSM_RS15785"
                     /old_locus_tag="pgaptmp_003157"
     CDS             9856..10842
                     /gene="ddpD"
                     /locus_tag="ACHOSM_RS15785"
                     /old_locus_tag="pgaptmp_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4559628.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTLQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADIIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            10835..11761
                     /gene="ddpF"
                     /locus_tag="ACHOSM_RS15790"
                     /old_locus_tag="pgaptmp_003158"
     CDS             10835..11761
                     /gene="ddpF"
                     /locus_tag="ACHOSM_RS15790"
                     /old_locus_tag="pgaptmp_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HGU4559629.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDNSVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(11817..12248)
                     /gene="osmC"
                     /locus_tag="ACHOSM_RS15795"
                     /old_locus_tag="pgaptmp_003159"
     CDS             complement(11817..12248)
                     /gene="osmC"
                     /locus_tag="ACHOSM_RS15795"
                     /old_locus_tag="pgaptmp_003159"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HGU4559630.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            12611..12826
                     /gene="bdm"
                     /locus_tag="ACHOSM_RS15800"
                     /old_locus_tag="pgaptmp_003160"
     CDS             12611..12826
                     /gene="bdm"
                     /locus_tag="ACHOSM_RS15800"
                     /old_locus_tag="pgaptmp_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HGU4559631.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAQHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            12928..13065
                     /gene="sra"
                     /locus_tag="ACHOSM_RS15805"
                     /old_locus_tag="pgaptmp_003161"
     CDS             12928..13065
                     /gene="sra"
                     /locus_tag="ACHOSM_RS15805"
                     /old_locus_tag="pgaptmp_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HGU4559632.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            13222..14919
                     /gene="maeA"
                     /locus_tag="ACHOSM_RS15810"
                     /old_locus_tag="pgaptmp_003162"
     CDS             13222..14919
                     /gene="maeA"
                     /locus_tag="ACHOSM_RS15810"
                     /old_locus_tag="pgaptmp_003162"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU4559633.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            15055..16065
                     /gene="adhP"
                     /locus_tag="ACHOSM_RS15815"
                     /old_locus_tag="pgaptmp_003163"
                     /pseudo
     CDS             15055..16065
                     /gene="adhP"
                     /locus_tag="ACHOSM_RS15815"
                     /old_locus_tag="pgaptmp_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            16211..16495
                     /gene="yddM"
                     /locus_tag="ACHOSM_RS15820"
                     /old_locus_tag="pgaptmp_003164"
     CDS             16211..16495
                     /gene="yddM"
                     /locus_tag="ACHOSM_RS15820"
                     /old_locus_tag="pgaptmp_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU4559634.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(16546..17199)
                     /gene="fdnI"
                     /locus_tag="ACHOSM_RS15825"
                     /old_locus_tag="pgaptmp_003165"
     CDS             complement(16546..17199)
                     /gene="fdnI"
                     /locus_tag="ACHOSM_RS15825"
                     /old_locus_tag="pgaptmp_003165"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HGU4559635.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(17192..18076)
                     /gene="fdnH"
                     /locus_tag="ACHOSM_RS15830"
                     /old_locus_tag="pgaptmp_003166"
     CDS             complement(17192..18076)
                     /gene="fdnH"
                     /locus_tag="ACHOSM_RS15830"
                     /old_locus_tag="pgaptmp_003166"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HGU4559636.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(18089..21136)
                     /gene="fdnG"
                     /locus_tag="ACHOSM_RS15835"
                     /old_locus_tag="pgaptmp_003167"
     CDS             complement(18089..21136)
                     /gene="fdnG"
                     /locus_tag="ACHOSM_RS15835"
                     /old_locus_tag="pgaptmp_003167"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(20549..20551),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU4559637.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            21368..22249
                     /gene="yddG"
                     /locus_tag="ACHOSM_RS15840"
                     /old_locus_tag="pgaptmp_003168"
     CDS             21368..22249
                     /gene="yddG"
                     /locus_tag="ACHOSM_RS15840"
                     /old_locus_tag="pgaptmp_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HGU4559638.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGVNGITVFVLLTGASLWVYYFLTQQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            22508..22783
                     /gene="yddL"
                     /locus_tag="ACHOSM_RS15845"
                     /old_locus_tag="pgaptmp_003169"
                     /pseudo
     CDS             22508..22783
                     /gene="yddL"
                     /locus_tag="ACHOSM_RS15845"
                     /old_locus_tag="pgaptmp_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            22822..>23460
                     /locus_tag="ACHOSM_RS15850"
                     /old_locus_tag="pgaptmp_003170"
     CDS             22822..>23460
                     /locus_tag="ACHOSM_RS15850"
                     /old_locus_tag="pgaptmp_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGU4559639.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNTLESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCECLQYLNLSHNQLSDIVAGN"
BASE COUNT         5888 a         5911 c         5863 g         5798 t
ORIGIN      
        1 tacgagaatg agcgcacatc tgtttaccgg aaaccagcac atatggatat ctgctcccga
       61 aacaagaaat taacgattag aagaccagcg atactggttg cactggcact tttactgtgt
      121 agttgtaaaa gcacgcctcc agagtccatg gtgacaccac cagcaggttc aaagccacca
      181 gccacgacgc aacaatcgtc acaaccgatg cgtggcatct ggctggccac ggtgtctcgc
      241 ctcgactggc caccggtttc ctcggttaac attagtaacc ccaccagccg ggcccgtgta
      301 caacaacagg cgatgatcga caaactggat catctgcaac gtctcggtat aaacacggtc
      361 tttttccagg tcaagccgga cggtaccgcc ctgtggccat cgaaaatttt gccgtggtcc
      421 gatcttatga ccggtaagat tggtgaaaat ccgggttacg atccgctgca atttatgctc
      481 gacgaagccc acaagcgtgg gatgaaagta cacgcctggt ttaaccccta tcgcgtatcg
      541 gttaatacga agcccggtac tatcagggaa ctgaatagca ctctgtctca acaaccggcg
      601 agcgtctatg tgcaacaccg tgactggatc agaacgtctg gcgatcgctt tgtcctcgac
      661 ccgggcatcc ctgaggttca ggactggatc acatcaatag tcgccgaagt ggtttcccgc
      721 tatccggtag atggcgtgca gtttgacgac tatttctata cggagtcacc gggttcacgg
      781 ctaaatgata acgaaacgta ccgtaaatac ggaggcgcat ttgcgtcaaa agcagactgg
      841 cggcgcaaca atactcagca gttaattaca aaagtatcgc acaccattaa aagcattaag
      901 ccgggagtcg aatttggcgt tagcccggca ggcgtgtggc gtaaccgatc acacgatccg
      961 ctcggttccg atacccgagg cgcggcagcc tatgacgaat cctacgctga cacccgtcga
     1021 tgggtggaac aaggattgct ggattacatt gctccccaaa tttactggcc gttctcacgg
     1081 agtgccgcgc gttatgacgt gttggcaaaa tggtgggcgg atgtcgttaa accgaccagg
     1141 acccgcctgt atatcggtat cgccttctat aaagtgggtg aaccttcaaa gatagagcca
     1201 gactggatga ttaacggcgg cgtaccggaa ctgaaaaagc agctcgatct taacgatgcg
     1261 gtaccagaaa ttagcggcac catcttgttc cgtgaggact atctgaataa accgcagact
     1321 caacaagcgg tcagctatct gcaaagtcgt tggggcagtt aaactcccat gcttactttg
     1381 taacaagccg gtgctttacc caccggcttg ttacaccttg tgaaatatcc cactagtaat
     1441 catgcttaca taagtcaaat taaccacgca attaaccaca cttagttaaa cagctataaa
     1501 tccctaataa aaatgtatga atatgaaaaa ccatacgata ttcgctgtca ccgaaaaata
     1561 attacgatgg gctatactct tctgaagaac agtagcgtag attgaaactg tcctaagtcg
     1621 cttatttctt ttcagtatat cttcatattt caggagaata tggtgcagtt agtcgatctc
     1681 gcacgttgcg ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga
     1741 tgtattttaa aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatccactca
     1801 ttcgtgctaa agccgcggaa attgccgttg cgcatgctca ttatctgagt atcgagtttt
     1861 atcgaattgt ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc
     1921 ggcagttgaa aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg
     1981 atgtcgaaag gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa
     2041 ttccggtaga aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa
     2101 tcttctcttc ggattattct gccgcagaaa aacttcaggt ctatcatttc tcgattaaca
     2161 gtattgatat cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct
     2221 caaaggaaga tgaaaactat cgtatcttct cgttgctgga aaacgccgaa gaagaaaaag
     2281 aacggcaaat agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg
     2341 attctgattt aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc
     2401 ataaaggtcg acattatttt agcggcattg ccgaagtagg ccatatctcc cgtctgattc
     2461 aggatttcga cggtattttc aatcaaacca tgcgtaacac caggaatttg aataacagaa
     2521 gtttgcgggt gaagtttcta ttacagataa gaaataccgt atcacaaatt attaccttgc
     2581 tgcgtgaatt gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat
     2641 tacttaaccg ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga
     2701 ccggtacacc gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata
     2761 cgtggggcca taacactggc gatgaaattc tgcgtaaagt ctctcaggcc ttttatgaca
     2821 acgtccgcag tagtgattat gttttccgct acgggggcga tgaatttatc attgttttga
     2881 ctgaagcttc ggaaaacgaa acgttacgta ccgcagaacg tattcgcagt cgggtggaga
     2941 aaaccaaact gaaagccgca aacggcgagg atattgccct ctcactttcc atcggtgccg
     3001 ccatgtttaa tggtcatcct gactatgagc gcctcattca aatagccgat gaagctctgt
     3061 atatcgccaa aagacgaggt agaaaccgtg ttgaactctg gaaagccagt ctttagatgc
     3121 gccaggatgc agaggtaatc atgaagctaa ccgatgcgga aaatgccgcc gatggcattt
     3181 ttttccccgc ccttgagcaa aatatgatgg gtgcggtgtt aattaacgaa aatgatgaag
     3241 tgatgttttt caaccccgcc gcagagaagc tctgggggta caaacgtgaa gaagtcattg
     3301 gcaataacat tgatatgctg attccgcggg atttgcgtcc tgcgcatcct gaatatattc
     3361 gtcataaccg tgaaggcggt aaagcgcgcg tcgaggggat gagtcgggag ctgcagctgg
     3421 agaaaaaaga cggcagtaaa atctggaccc gttttgcgct atcgaaagtg agcgccgagg
     3481 ggaaagttta ttacctggcg ctggtacggg atgccagcgt agaaatggcg caaaaagaac
     3541 agacccgaca attgattatt gccgttgacc atctcgaccg accggtgatt gtcctcgatc
     3601 cggaacgcca tattgtgcag tgcaatcgcg catttaccga aatgtttggt tactgcatta
     3661 gcgaagccag cggtatgcag cccgatacac tcctgaacat tcctgaattc cctgccgata
     3721 accgcattcg tttacaacag ttgctatgga aaaccgcccg cgatcaggac gaatttctgc
     3781 tgttgacgcg caccggtgaa aaaatctgga ttaaagcctc tatcagcccg gtttatgacg
     3841 tgctcgcgca tctgcagaac ctggtaatga ctttctcgga tatcaccgaa gaacggcaga
     3901 ttcgccagct tgaaggcaat attctcgccg ccatgtgcag cagcccgcca tttcatgaaa
     3961 tgggggaaat catttgtcgt aacatcgaat ctgtactcaa cgaatcgcat gtttcgctgt
     4021 tcgcactgcg caacgggatg ccgatacact gggcgtcatc ttcccacggt gcagaagctc
     4081 aaaatgcgca aagctggtca gcgaccattc gtcagcgtga tggcgcgcct gcggggatcc
     4141 tgcaaattaa aacctcgtca ggagcagaaa ccagcgcctt tatcgaacgc gtggcagata
     4201 tcagccagca tatggccgcg ctggcgctgg aacaggaaaa aagccgtcag catattgaac
     4261 aactcatcca atttgatccg atgaccggtc tgccaaatcg caataacctg cacaattacc
     4321 tcgatgacct ggtcgacaaa gccgtctctc cggtggtgta tctcatcggt gttgaccata
     4381 ttcaggatgt gattgatagc cttggctatg cgtgggccga tcaggcattg ctggaagtgg
     4441 tcaatcgctt tcgtgaaaaa ctcaaaccgg atcagtatct ctgtcgtatc gaaggtacgc
     4501 agtttgtcct cgtgagcctg gaaaacgacg tcagtaacat tacccagatc gccgatgagc
     4561 tacggaatgt ggtcagcaag ccgataatga ttgacgataa accattcccg cttaccttga
     4621 gtattggcat cagctacgac gtgggtaaaa accgcgatta cctactctcc actgctcaca
     4681 atgcaatgga ttatattcgc aagaatggcg gtaacggctg gcagttcttc agcccggcga
     4741 tgaacgaaat ggtaaaagag cgtttggttt taggcgcagc gctgaaagaa gcgattagca
     4801 ataaccaact gaaacttgtt taccagccgc aaatcttcgc agaaacgggt gaactgtacg
     4861 gcatcgaagc ccttgcgcgc tggcacgatc cccagcatgg tcatgtgccc ccttcacggt
     4921 ttattcctct cgcagaagag attggtgaaa tcgaaaatat tgggcgctgg gtcatcgcgg
     4981 aagcttgccg tcagttagca gaatggcgta gccagaatat tcatatcccg gcgttatccg
     5041 tgaacttgtc ggcgctgcac tttcgcagta atcagctgcc taatcaggtg tctgatgcaa
     5101 tgcaagcctg gggtattgac ggccaccagc tgacggtaga aatcacggaa agcatgatga
     5161 tggaacacga taccgaaatc tttaagcgca ttcagatcct gcgtgatatg ggcgtgggtt
     5221 tatcggtaga tgattttggc acgggctttt ccggattatc ccgcttagtc agtcttccgg
     5281 taacggaaat caaaattgac aaaagttttg tcgatcgttg tctgacggaa aaacgcatcc
     5341 ttgccttact tgaagccatt accagcattg ggcaaagcct caatttaacc gtcgtggcgg
     5401 aaggcgtcga aaccaaagag caatttgaga tgctacgcaa gatccactgt cgcgttattc
     5461 agggatattt cttttcccgc ccccttcccg ccgaagaaat tccaggctgg atgagcagcg
     5521 tgttaccgct gaaaatctga caaattcctc tcgcccgcac tcgcgggttt tcctttaagg
     5581 tgacactgtc acttaaatac tgtgatttca gctacgattc cgggagattc cttccttaac
     5641 ttcccctctt gcaccaaaac tgcactacgc tgcacaaatg ttgaacaaaa gttaaaaata
     5701 taactttaag ccagcattct ggcgcttatc ccggcatggc atgagatctg cataagcgga
     5761 aagcgcagca atttttgtct tatacaggaa ccgtttatgt cggataccac cgaactggtt
     5821 gatttagccg tgatcttccc tgatctggag atcgaattga aatatgcctg cgttgataac
     5881 atcacaggta aagctattta tcagcaagcg cgttgtctgc tacataaaga tgcgattacc
     5941 gcgctggcga aaagtatcag tatcgcccag ctgtcaggct tacaactggt gatttacgat
     6001 gcgtatcgcc cacaacaagc acaggcgatg ttgtggcaag cctgcccaga cccgcaatat
     6061 gttgttgatg tgacggtcgg ttctaatcac agccgtggca cagcgctcga cctgacgctt
     6121 cgtgatgagc acgggaacat cctcgatatg ggcgcagggt tcgatgaaat gcacgagcgc
     6181 tcccatgcct atcacccttc cgtcccgccc gccgctcagc gcaatcggct gttgctgaat
     6241 gcgattatga ctggtggcgg ttttgtcggt atctccagcg aatggtggca cttcgaatta
     6301 ccccaggcag cgagttaccc actgctcgct gatcaattca cctgttttat atcccctggc
     6361 acacagcacg tcagtcaatc aggaaactgt catgaagaga tcgataccgt ttcgtcctac
     6421 attgctcgcg ctcgtccttg ccacaacttt cccggttgcg cacgccgccg taccgaaaga
     6481 tatgctggtg attggtaagg ccgccgatcc acaaaccctc gacccggcgg taacaataga
     6541 taataacgac tggacggtga cctacccgtc ttatcagcgg ctggttcagt acaaaacgga
     6601 cggtgataaa ggctcaaccg acgttgaagg cgatctggca agtagctgga aagcgtctga
     6661 cgatcaaaaa gagtggacgt tcaccctgaa ggacaatgct aaatttgccg atggcacacc
     6721 ggtcactgcc gaagcagtaa aactctcttt tgagcggcta ctaaaaatcg gccaggggcc
     6781 agcagaagca tttcccaaag atttaaagat tgatgctccc gacgaacata cggtgaagtt
     6841 tacccttagc cagccattcg caccgttcct ctacacgctg gcgaatgacg gtgcatccat
     6901 tatcaatccg gcggtgttaa aggaacatgc agcggatgat gcccgcggct tcctcgcgca
     6961 aaataccgcc ggttccggac catttatgct gaaaagctgg caaaaaggtc agcaattagt
     7021 tctggtgcca aatccgcatt accctggcaa taaaccgaac tttaagcgag tatcggtaaa
     7081 aattattggt gaaagtgcct cccgtcgcct gcagctctcc cgtggtgata ttgacattgc
     7141 cgatgcgctg ccggtggatc aactcaacgc cctgaagcag gaaaacaaag tcaatgtggc
     7201 agagtatccg tcactgcgcg tcacctacct gtatctgaat aacagcaaag cgcctcttaa
     7261 tcaggcggat ctgcgtcggg ccatttcctg gtctaccgat tatcagggca tggttaacgg
     7321 cattctgagt ggtaacggaa aacagatgcg cggcccgatt ccggaaggca tgtggggcta
     7381 cgatgcgacg gcaatgcaat acaaccatga cgaaacgaaa gccaaagccg aatgggataa
     7441 agtgacgagc aaacccacca gcctgacgtt tctctactcc gataacgatc cgaactggga
     7501 gcctattgct ctggcgacac aatccagtct caacaagctg ggcatcaatg tgaagctgga
     7561 aaagctggcg aacgccacca tgcgcgacag agtgggtaaa ggtgattacg acattgcgat
     7621 tggcaactgg agtccggatt ttgccgaccc gtatatgttt atgaattact ggtttgagtc
     7681 agacaaaaaa ggtctgccgg gtaaccgctc gttctatgaa aacagtgagg tcgataagtt
     7741 actgcgcaat gcgcttgcga ccaccgacca gacgcagcgt acccgggact accagcaggc
     7801 acagaaaatc gtcattgatg acgctgctta tgtgtacctg ttccagaaaa actaccaact
     7861 ggcgatgaac aaagaggtga aaggctttgt gttcaatccc atgctggaac aggtcttcaa
     7921 tatcaatacc atgagtaaat aacgtgacct tctggagtat tttacgccaa cgctgctggg
     7981 ggctggtgct cgtggtggcg ggcgtctgcg tgattacgtt tattatctcg catctgatcc
     8041 ctggcgatcc ggcgcggtta ctggcgggtg accgcgccag cgatgctatc gtggaaaata
     8101 ttcgccagca actgggactg gaccagccac tgtatgtaca gttttaccgc tacgtcagcg
     8161 acctgtttca tggtgacctg ggaacatcca ttcgtaccgg gcgtccggtg ctggaagagt
     8221 tgcgtatatt tttcccggcg acgctggaac tggctttttg tgccctgctg ctggcactcc
     8281 tgattggcat cccgctgggc atactctctg cagtctggcg aaatcgctgg ctggatcatc
     8341 tggtgcgaat aatggccatt accggaatct ccacacctgc gttctggctt ggactgggcg
     8401 tcattgtgct gttttatggt catctgcaaa ttcttcccgg cggcggaagg cttgatgact
     8461 ggctggatcc accaacgcac gttaccggct tttatctgct cgatgcgctg cttgaaggca
     8521 acggtgaagt cttcttcaat gcgttgcaac atctcatctt accggcatta acgctggcgt
     8581 tcgttcacct gggaattgtc gctcgccaga tccgctcagc gatgctggaa caattgagtg
     8641 aagactacat tcgtaccgcc cgggccagcg gcttgcccgg ctggtatatc gttttatgtt
     8701 atgcgctacc caatgcgttg atcccatcga ttaccgtatt gggtctggcg ctgggcgatt
     8761 tgttgtatgg cgcagtgctc accgaaaccg tttttgcctg gcccggaatg ggtgcatggg
     8821 tagtaacatc aatacaggcg ctcgacttcc cggcagtgat gggctttgcc gtcgtggttt
     8881 catttgctta tgtgctggtc aacctggtgg tggatttgct ctatttgtgg attgatccgc
     8941 gtatcggacg tggaggtggt gaatgatgct aagcgaggaa acgtccgccg tacgcccaca
     9001 aaaacaaacg cgatttaacg gtgcaaaact ggtatggatg ctgaaaggca gtccgctcac
     9061 cgtgaccggc gcagtcatca ttgtattaat gctattgatg atgatttttt caccgtggct
     9121 tgcgacgcat gatcccaacg ccattgattt aaccgcccgc cttttgccgc cttctgcggc
     9181 gcactggttt ggcaccgatg aagtgggacg cgatctgttc agccgcgtac tggtcggcag
     9241 tcagcaatca attctcgccg gattagtcgt ggttgccatt gcgggtatga ttggttcgct
     9301 actcggatgt ctatccggtg tgcttggcgg acgcgcagac gccatcatca tgcgcatcat
     9361 ggacattatg ctgtcgattc cttcgttggt actgacaatg gcactggctg ccgctctcgg
     9421 gccgagttta tttaacgcca tgctggcgat tgctattgtg cgaattccct tttatgtgcg
     9481 cctggcgcgg gggcaagcat tagttgtacg ccagtatacc tatgttcagg cggcgaaaac
     9541 ctttggtgcg tcgcgttggc atctgatcag ctggcatatt ttacgtaact ccctgccgcc
     9601 gctgatcgtg caggcatcgc tggatatcgg tagcgcgatt ttaatggccg ccacgttggg
     9661 atttattggc ctgggtgctc aacaaccgag tgctgaatgg ggcgcgatgg tagcgaatgg
     9721 tcgcaactat gtactcgatc agtggtggta ttgcgcattt ccgggggcag cgattttgct
     9781 taccgccgtt gggtttaatc tctttggtga tggtattcgc gatctgcttg acccgaaagc
     9841 aggaggaaag cagtcatgac ccaacccgtt ctggacattc aacagctgca tttgagtttc
     9901 cccggtttta acggtgatgt tcacgcgctc aacaatgtgt ccttgcagat taaccgcggt
     9961 gaaattgtcg gtctggtggg agaatccggc tcaggtaaat cagtcaccgc aatgctgatt
    10021 atgcgtctgc tacagacggg cagttattgc gtacatcggg ggcagatttc actgctggga
    10081 gacgatgttc ttaacgctcg ggaaaaacag cttcgtcagt ggcgcggcgc acgagtggcg
    10141 atgatctttc aggaaccgat gaccgccctc aatccgacac gtcgaatagg tcttcagatg
    10201 atggacgtga tccgccatca tcaaccaata agtcgtcggg aagccagagc taaagcgatt
    10261 gccctgctgg aagagatgca aatcccggat gccgtggaag ttatgtcgcg ctatccgttt
    10321 gagctttcag gtggtatgcg ccagcgggta atgattgcgc tggcattctc ctgcgagccg
    10381 caattgatta ttgccgacga accgactacg gcgctggacg tcacgctaca gttgcaggta
    10441 ctgcgtttgc ttaaacataa agcccgcgcc agtggaactg cggtactgtt catcagccat
    10501 gatatggccg tggtgtcgca actgtgcgat agcgtttacg tgatgtatgc cggaagcgta
    10561 attgaaagcg gcgtgacggc agacattatc catcatcccc ggcatccgta taccattggt
    10621 ttgctgcaat gcgcaccaga acatggagta ccacgccagc cattacccgc cattccaggg
    10681 acggtaccaa acctcaccca tttgcctgac ggctgcgctt ttcgcgatcg ttgctatgcg
    10741 gcaggtgcac agtgtgaaaa cgtcccggcg ctgacagcgt gtggtgacaa caaccagcgc
    10801 tgcgcctgtt ggtatccgca gcaggaggtc ataagtgtct gacacgttat taacgttacg
    10861 cgacgtccat atcaatttcc cggcccgtaa aaactggctt ggtaaaacta cggaacatgt
    10921 tcatgccatt aatggtattg atttacagat ccgccgtggt gaaaccttag ggatcgtcgg
    10981 cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta atgggtatgc tgcaaccgag
    11041 ccacgggcag tacatccgtt ctggctcaca acgcattatg cagatggtgt ttcaggaccc
    11101 gctctcttcg ctcaatccgc gcttaccggt gtggcgcatc atcacagaac cgctctggat
    11161 agctaagcgt agtagtgaac aacagcggcg agcgttggca gaggagctgg ctgtgcaggt
    11221 gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg ttctccggcg ggcagcggca
    11281 acgtatcgcc attgccagag cactctcttc gcagcctgac gtgattgtgc ttgatgagcc
    11341 aacctctgcg ctggataact ccgtgcaggc gcagatcctc aatttactgg taacgctaca
    11401 ggaaaatcac gggctgactt atgtgctgat ttcacacaat gtctcggtga tacgtcatat
    11461 gagcgatcgg gtggcggtga tgtatctcgg gcagattgtg gaactagggg aagcgcagca
    11521 ggtgctgacg gcacctgcac atccatacac ccgattattg ctggattccc tccccgccat
    11581 tgataaaccg ctggaggaag aatgggcatt acgtaaaacg gatctgccgg gaaaccgcac
    11641 gttgccgcaa ggctgttttt tctacgaacg ttgccccctt gcaaccagcg gatgtgaagt
    11701 ccggcaatca ttagcaataa gggaggacgg acgtgagctc cggtgctggc gggcgctgta
    11761 gagatgctaa caacgcatca ggcattcacg cgcctgacac gtcatccggc aatgctttac
    11821 gatttcaact ggtaatccag cgtaatttcc gctttcagta cctgagagac cgggcatcct
    11881 gcttttgctt tctggatgat gccgtcaaag gtagaggcat caatacccgg caccgcaact
    11941 tcactcttca gtgcgatttt cgtaatcgca aaaccggcat ccactttatc cagcgacaca
    12001 tcggcggtgg tatcaatcga tgttggcgtg aatcccgctt cccccagcat taatgaaagc
    12061 gccattgaga aacatgcggc atgcgctgcg ccaatcagtt cttcagggtt ggttcctttt
    12121 tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt tcagcacgcc actctcggtg
    12181 gatactgttc ccttcccgcg tttgatatcg ccttcccagt gtgcctgacc tttcttatgg
    12241 attgtcattg ttgctctcct gtgggcttaa aaaggaaatt atagactacg tgagaaacag
    12301 cacgaggata agcaggatat tccgaataaa atccggcgaa aatattactt ctgcacttaa
    12361 attaaattcc cacaataaat taaattccca caatagatta ccgggaatat ataacccaaa
    12421 agatattgta tcaatattaa tcggatcata ccaatcacaa tccctatgaa tatcatggtg
    12481 ttaaaatata aaaatcatca accaggacta atcttaacaa cgaagcggca aatatttgcc
    12541 atgctgaatg tgctttataa atctgcgatc cgtagcagac accataaata cacagacacg
    12601 gagaatcact atgtttactt attatcaggc agaaaattca acagcagaac ccgctctggt
    12661 taatgccatt gagcaaggcc ttcgggcaca gcacggtgtt gtcactgaag atgacattct
    12721 catggagctg accaaatggg tggaagcttc tgataacgac atcctcagtg atatctacca
    12781 gcaaacgatc aattatgtgg tcagtggcca gcaccctacg ctttaaggtg ctatgcttga
    12841 tcggcaacct aatttagggg tttagcacgt gtttcttcgc tacggcgatg ttgtccttaa
    12901 aactagctac aggattgagg agttaaaatg aaatcgaacc gtcaggcacg tcatattctt
    12961 ggactggacc ataaaatttc taaccagcgc aaaatagtta ccgaaggtga caaatccagc
    13021 gtagtaaata acccaaccgg cagaaaacgc cctgctgaaa agtaattcat aaccatcagt
    13081 cctcaatgac gattaaacac cattgcctgc gcaatggtgt ttttgttttt atctgcttta
    13141 tacttggggc cgacgccctg gcggtaaagc aaagacgata aaagcccccc agggataaat
    13201 attcaaaaaa gagtgagtga catggaacca aaaacaaaaa aacagcgttc gctttatatc
    13261 ccttacgctg gccctgtact gctggaattt ccgttgttga ataaaggcag tgccttcagc
    13321 atggaagaac gccgtaactt caacctgctg gggttactgc cggaagtggt cgaaaccatc
    13381 gaagaacaag cggaacgagc atggatccag tatcagggat tcaaaaccga aatcgacaaa
    13441 cacatctacc tgcgtaacat ccaggacacc aacgaaaccc tcttctaccg tctggtaaac
    13501 aatcatcttg atgagatgat gcctgttatt tataccccaa ccgtcggcgc agcctgtgag
    13561 cgtttttctg agatctaccg ccgttcacgc ggcgtgttta tctcttacca gaaccggcac
    13621 aatatggacg atattctgca aaacgtgccg aaccataata ttaaagtgat tgtggtgact
    13681 gacggtgaac gtattctggg gcttggtgac cagggcatcg gtgggatggg cattccaatc
    13741 ggtaaactgt cgctctatac cgcctgtggc ggcatcagcc cggcgtatac ccttccggtg
    13801 gtgctggatg tcgggaccaa caaccagcag ctgcttaacg atccgctgta tatgggctgg
    13861 cgtaatccac gtatcactga cgatgaatac tatgaattcg ttgatgaatt tatccaggct
    13921 gtgaaacaac gctggccgga cgtgctgttg cagtttgaag actttgctca aaaaaatgcg
    13981 atgccgttac ttaaccgcta tcgcaatgaa atttgttctt ttaacgatga cattcagggc
    14041 actgcggcgg taacagtcgg cacactgatc gcagccagcc gcgcggcagg tggtcagtta
    14101 agcgagaaaa aaatcgtctt ccttggcgca ggttcagcgg gatgcggcat tgccgaaatg
    14161 atcatcgccc agacccagcg cgaaggatta agcgaggaag cggcgcggca gaaagtcttt
    14221 atggtcgatc gctttggcct gctgacggac aagatgccga acctgctgcc tttccagacc
    14281 aaactggtgc agaaacgcga aaacctcagt gactgggata ccgacagcga tgtgctgtca
    14341 ctgctggatg tggtgcgcaa tgtaaaacca gatattctga ttggcgtctc aggacagacc
    14401 gggctgttta cggaagagat catccgtgag atgcataaac actgtccgcg tccgatcgtg
    14461 atgccgctgt ctaacccgac gtcacgcgtg gaagccacac cgcaggacat tatcgcctgg
    14521 accgaaggta acgcgctggt cgccactggc agcccgttta atccagtggt atggaaagat
    14581 aaaatctacc ctatcgccca gtgtaacaac gcctttattt tccctggcat cgggctgggt
    14641 gttattgctt ccggcgcgtc acgtatcacc gatgagatgc tgatgtcagc aagtgaaacg
    14701 cttgctcagt attcgccgct ggtgctgaac ggcgaaggtc tggtactacc ggaactgaaa
    14761 gatattcaga aagtctcccg cgcaattgcg tttgcggttg gcaaaatggc gcagcagcaa
    14821 ggcgtggcgg tgaaaacgtc tgctgaagct ttgcaacaag ccattgacga taatttctgg
    14881 caagccgaat accgcgacta ccgccgtacc tccatctaag cctgcgcccg gtagtgaagg
    14941 ctaccgggct atttccctct ccctttttca gatctcatcc atactggggg tagtggcgaa
    15001 taaatctcat ttgcctcacc tgctatgcag aacatcatcc gaaaaggagg aactatgaag
    15061 gctgcagttg ttacgaagga tcatcatgtt gacgttacgg ataaaacact gcgctcactg
    15121 aaacatggct aagccctgct gaaaatggag tgttgtggtg tatgtcatac cgatcttcat
    15181 gttaagaatg gcgattttgg tgacaaaacc ggcgtaattc tgggccatga aggcatcggt
    15241 gtggtggcag aagttggccc aggtgtcacc tcattaaaac caggcgatcg tgccagcgtg
    15301 gcgtggtttt acgaaggatg cggtcattgc gaatactgta acagtggtaa cgaaacgctc
    15361 tgccgttcag ttaaaaatgc cggatacagc gttgatggcg ggatggcgga agagtgcatc
    15421 gtggtcgccg attacgcggt aaaagtgcca gatggtctgg actcggcggc ggccagcagc
    15481 attacctgtg cgggggtcac cacctacaaa gccgttaaac tgtcaaaaat tcgtcccggt
    15541 cagtggatta ctatctacgg tcttggcggt ctgggtaacc tcgccctgca atacgcaaag
    15601 aatgtcttta acgccaaagt gatcgccatt gatgtcaatg atgagcagtt aaaactggca
    15661 accgaaatgg gtgcagattt agcgattaac tcacgcaccg aagacgccgc caaaattgtg
    15721 caggagaaaa ccggtggcgc tcacgctgcg gtggtaacag cagttgctaa agctgcgttt
    15781 aactcggcag ttgatgctgt ccgtgcaggc ggtcgtgttg tggctgtcgg tctgccgccg
    15841 gagtctatga gcctggacat cccacgcctt gtgctggatg gcattgaagt ggtcggttcg
    15901 ctggtcggca cgcgccagga tctaaccgaa gccttccagt ttgccgccga aggcaaagtg
    15961 gtgccgaaag tcgccctgcg tccgttagcg gacatcaaca ccatctttac cgagatggaa
    16021 gaaggcaaaa tccgtggccg catggtgatt gatttccgtc actaagaggc ctttgctgcg
    16081 actgccatgt tcgggtcgca gcatcgcaca ctctccaaca tgaaatggct aaatggatta
    16141 ttgttgcgta ggaaggaaca ataatgatag tgttcccaga gaaggaacgc tactgagagt
    16201 aaagattttg atgaaaatgg ccaatcatcc ccgcccgggg gacattattc aggaatcact
    16261 ggacgaactt aatgtcagcc tgcgcgagtt tgccagagca atggaaattg cgccctcaac
    16321 ggcaagccga ttactgaccg gaaaagcagc tttgacgcca gaaatggcga ttaaactttc
    16381 cgtggtgatc ggcagttcgc cgcaaatgtg gctgaatctg caaaacgcct ggagtctggc
    16441 agaggctgaa aaaacggtgg atgtgtcgcg actgcgccgt ctggttacgc aataaaaaaa
    16501 tggcgcttct taaagcgcca ttttcagctt aaagttcaaa agagattata tcccttcttc
    16561 actctctttt ttcgcttctg ccttctcgat ttcacgatac cagcgcggat ggtgtttctt
    16621 cgcccagcga cggctcacct tcccttcgat catcccttta atcgaccctt tcacccagaa
    16681 tgccatatac atatggatca ggatggcgtg gatcaggatg atacccgcag ccgcgtggat
    16741 cagcaggctg tagcgaacaa cctgcatcgg gaagtactgc gcaaagtacg gacgccagat
    16801 aatcaccccg gtcaccagca gcacgaaaat catgctcatg atcgaccaga acatcatctt
    16861 ttgcccggcg ttgtacttac caacatccgc cactttgtgc tcattgcctt ttaatacttc
    16921 gacaatgttc aacagccacg gaatatcttt cttatccggg atgttgtgat gcacaaaacg
    16981 cacaaacata aacatcagtg cgacgaaaat cgcaatgccg aagaacgggt gcaaaatgcg
    17041 tcccatctgc ggcgtaccga aggtttgcgt cagccattgc agcgtcggga agaagaacga
    17101 aatcccggac agcgccacca ggaagaagca aatcaccacg gtccagtgac aggcgcgatc
    17161 aataaatttg gtgcgcacaa tcattttcga cttactcatg atgatcctcc tcgtcatcgt
    17221 ccacttcctt attcgggcca ataccgatgt agtggaaaat caacccggca aaagtggcaa
    17281 taaagccagc cgctgccagc ggtttcaacg cacctttcca caggcttacc gaggtgtcga
    17341 tcttcggatc tttcggcaga ccgtgataca gctccggctg atcggcgtga tgcagcacgt
    17401 acataacgtg cgtaccaccg accccttccg ggttgtagac gccagcatgt tcgtaaccac
    17461 gcgctttcag tttcgccacg cgctgttccg ccagctccag catctccttc ttggtgccga
    17521 agtggatagc cccggtcgga caggttttca cacaagccgg ttcctggccg acgctgacgc
    17581 gatcgacgca gagcgtgcat ttatataccc ggttatcctc tttgttgagg cgcggaatat
    17641 taaacggaca cccggcaatg cagtaaccac agccgatgca gttttccgac tggaaatcga
    17701 caatcccgtt agcgtactga atgattgcac cagcagacgg gcacgccttc aggcagccgg
    17761 gatcttcaca gtgcatacag ccgtctttac ggatcagcca ctccagcttg ccgttctgtt
    17821 cggtttcgct aaagcgcatc accgtccagg acttggcgct cagatcggcg gggttatcgt
    17881 aaaccccgac gcagtgcccc acttcatcac ggatgtcgtt ccactccgaa cacgccacct
    17941 gacaggcttt acagccgata caggtggaaa cgtcgataag ttttgcgact tctgctttgt
    18001 aatcacgcac ctgagaaggc ggcgtgatgg agttagttgc ggaccttttg ataatgtcct
    18061 gcgtttccat agccatctgt tcgccccctt acgccttctc gatgttgact aagaacgctt
    18121 tatattccgg cgtttgcgag tttgcatcac cgacattcgg cgtcagagtg ttagcgatgt
    18181 aacctttacg cgcgacaccc tcaaagcccc agtggattgg aatacccacc gtttcaacct
    18241 gctgaccatt tacattcagc ggtttcagac gacgcgttac cacagccacc gcgcggataa
    18301 agccacgctt gctggagaca gtgacacgat cgccattatt aatgcctttc gccgccgcca
    18361 gcgtttcgct gatttccaca aactgttccg gctgagcaat tgcgttgagc aatgcgtgct
    18421 tggtccaggt gtggaagtgc tcggtcagac gataggtcgt acccacatac gggaactgct
    18481 cttttttacc catccgcagc gcgtcttgtt catatagacg aacaaccggg ttagacacca
    18541 cgttcggatg cagcgggtta gtgcccagcg gcgtttcaat cggctcgtag tgttccggga
    18601 acggaccttc cgccattttg ttgatggcaa acaggcgtcc catcccttcc ggctgcatga
    18661 taaacggccc ggttggcgta cccggtgcgg cattgccgaa gtcaggaata tcgttacccg
    18721 tccacttgct gccgttccac tggatcagca tccgtttcgg atcccacggt ttaccgttga
    18781 tatccgccga agcacggttg tacagcacgc gacggttgag cggccacgcc caggcccatc
    18841 ccagcgtatt ccccagaccg gacgggtcgg agttatcgcg gttagccatc tggttgccct
    18901 gctctgtcca gctaccggtg tagatccagc aagaagatgc ggttgtaccg tcatcacgca
    18961 gatgcgcaaa gctactcagc aactgacctt tcttcgcaat aagcacgcca ttagcgtcat
    19021 agagatcttc cagcgcgtag ccgttgttct ctttagccac ttcgtcagat tgcggttcgt
    19081 gcggctgctt gtagttccag ctcatcttca tcagcggttc tacgccttta ccaccttcgg
    19141 cctggtacag ctcgcgcaga tgatggtaga tacccgccag aatttcgccg tcgttacgcg
    19201 cttcgcccgg cgcgtcctga cctttccagt gccactgcaa ccagcgaccg gagttagcga
    19261 tagaaccatc ttcttcggca aagcaggtcg aaggcagacg gaatacttca gtctgaatag
    19321 acgccggatc gacatcgttc gactcgccgt ggttctgcca gaaggtagag gtttcagtca
    19381 ccagcggatc gataaccacc atgtacttca gcttgctcag gcagctcacc actttgtttt
    19441 tgtccgggaa ggacgcaacc gggttaaagc cctggcagaa ataaccggtg actttgcctt
    19501 cgtccatcat gttgaaatac ttgatgacgt cgtaggtctg gtcccacttc ggcagccagt
    19561 catagcccca gttgttctct ttctgcgcgg catcgccata gaaagatttc atcaggctaa
    19621 cgaagaactt cggatagttg ctccagtagt tcacctgacc agccagcgtc gctttcggcg
    19681 tgttcgcttc cagatacgac tgcaaatcaa cctgtttttc tgacggaagc gtcagataac
    19741 ctggcaggct ggtagagagc agacctaagt cagtcagccc ctgaatgttg gagtgaccac
    19801 gcaatgcgtt cacgccgcca ccggccatac ccatgttacc gagcagcaac tggatcatcg
    19861 ccatagtacg gatgttctgc gcacccacgg tgtgctgcgt ccagcccagc gcgtacagga
    19921 aggtggttgt gcgatccggt gcgctggtgg aggccagcac ttcacacact ttcaggaagt
    19981 cggcttttgg cgtaccgcag atgttttcaa cgacgtccgg cgtgtagcgg gaaacgtgcg
    20041 ctttcagcag gttccacaca cagcgcggat gagtcagtgt ttcatcgcgt tttgcatagc
    20101 cgttttcatc gaactgatag ttccaggacg acttatcata ctggcgtttt tcagcgtcgt
    20161 agccgctgaa caggccgtct tcgaaagcaa aatcatcacg caccagcagg ctggcgttgg
    20221 tgtaatgttt aacgtattcg gcgttgattt tgttgttttc gatcaggtag cgcaaaacgc
    20281 cagacaggaa cgtaatgtcc gtaccggaac gaataggtgc gtaaatatcc gccacagaag
    20341 cggtacgcgt aaaacgagga tcgacaacga tcaaggttgc atcgttgttg tttttcgctt
    20401 ccatcgccca gcggaaaccg acgggatgcg cttcagcagc gttaccgccc attaccatta
    20461 cgacgttagc gtttttgata tccacccagt ggttggtcat cgcaccgcga ccaaatgttg
    20521 gagcaagact tgctaccgtt ggtccgtgtc agacgcgcgc ctggttgtct accgccagca
    20581 tcccgaggga gcgggcaaat ttctgtgtca gcatcccggt ttcgttgctg gcaccggagg
    20641 cacacagcat accggtagaa agccaacggt ttaccgttac gccctgctcg ttcttttcaa
    20701 taaagttagc gtcacggtca gctttcatca gctttgcaat acgggagaat gcttcttccc
    20761 agctaatgcg ctgccatttg tcagaacctg gcgcacgata ttccgggtag cgcagacggt
    20821 tttcgctgtt gacgtaatcc agcaaaccgg ccccttttgg gcacagcgca ccacggctta
    20881 ccggatgatc cgggtcacct tcaatgtgat aaatcgcttc tctggcgttt ttcgcgccat
    20941 cacccaggct atacatcaat agcccgcaac ctacggaaca gtatgtgcag gtgttacgga
    21001 tctctttagc gcgtaataat ttgtagtttc gcgcctgagc cagtgcttgc ttaggggcaa
    21061 agcccaatgc cgctaccgtt gttccagcca taccgcccgc gcagatttta aaaaattgtc
    21121 tgcgactgac gtccattgct ttcctctttt ttcaggggta ttactgcgcg aggaaactaa
    21181 cagtaaaatt ctgaatcttt ttgcgcgaaa tcaaaaagaa cacgtgtttg ccacaaaata
    21241 atccgcctgc gcaaattgac ctacctcaat agcggtagaa aaacgcacca ctgcctgaca
    21301 ggccagtaaa aaaaatgcta taaaattcag cttaattttt aacggcatga gagacaaaac
    21361 agcgagcatg acacgacaaa aagcaacgct catagggcta atagcaatcg tcctgtggag
    21421 cacgatggta ggattgattc gcggtgtcag cgaggggctc ggcccggtcg gcggcgcagc
    21481 tgctatctat tcattaagcg ggctgctgtt aatcttcacg gttggatttc cgcgtattcg
    21541 gcagattccg aaaggctatt tactcgccgg gagtctgtta ttcgtcagct atgaaatctg
    21601 tctggcgctt tccttagggt atgcggcgac ccgtcatcag gcgattgaag tgggtatggt
    21661 gaactatcta tggcccagcc tgacaattct ctttgccatt ctgtttaatg gtcagaaaac
    21721 caactggctg attgtaccag gattattatt agccctggtc ggcgtctgct gggtgttagg
    21781 cggtgataat gggttacatt atgatgaaat catcaataat attaccacca gcccattgag
    21841 ttatttcctg gcgtttattg gtgcgtttat ctgggcagcc tattgcacag taacgaataa
    21901 atacgcacgc ggagttaacg gaattaccgt ttttgtcctg ctaacgggag caagtctgtg
    21961 ggtttactat tttcttacgc aacaaccaga aatggtattt agcacgcccg tcatgattaa
    22021 actcatctct gcggcattta ccttaggatt tgcttatgct gcatggaatg tcggtatatt
    22081 gcatggcaat gtcaccataa tggcggtagg ctcgtatttt acgcccgtac tttcctcagc
    22141 gcttgcagcc gtgctgctca gcgccccgct gtcgttctca ttctggcaag gcgcgctaat
    22201 ggtctgcggc ggttccctgc tctgctggct ggcgacacgt cgtggttaaa ataattgccg
    22261 ggtctacggc ccggcagtta atcagataaa ttccgacaat attaaaaaat aaatttcaaa
    22321 taccagtaat tcaccttata tctcatttcg acttcgatca catatcgata aataatatta
    22381 tttcatctat ttttctcatt gtttttaatc accattctat tttaaaaaca gtatgtcatt
    22441 tataaatggc gccgtttaaa attcgtttag aaaatacgct taacgataat taaaaggatt
    22501 ctgttaaatg aaattaaaaa tagttgcggt ggttgtaact ggtttgttag ctgcgaacgt
    22561 agcacacgct gccgaagtct ataacaagga tggtaataaa ctcgaccttt atggcaaggt
    22621 taccgctcta cgttatttta ctgatgataa gcgtgacgat ggtgataaaa cttatgcccg
    22681 tctcggcttt aaaggagaaa cgcaaatcaa tgatcaaatg attggttttg gtcactggga
    22741 atatgatttt aaaggctata acgatgaagc caacggctcg cgcggcaaca atctctgatt
    22801 gagttaagtg cccatcacaa tatgatcact gaccttatat tacacaatca tcccaggatg
    22861 aaaacaatca ctttaaacga caaccatatt gcacatttaa acgccaaaaa cactacaaaa
    22921 ctggaatatt taaacttaag caataacaat ttactgccaa ccaatgacat tgatcaacta
    22981 atatcatcaa aacatctttg gcatgtatta gttaacggca tcaacaatga tccacttgca
    23041 caaatgcagt actggactgc agtaagaaat ataattgatg acactaatga agtgaccatt
    23101 gatttatcag gacttaattt aaccactcaa ccaccagggc tgcaaaactt cacctctatc
    23161 aatcttgata ataaccaact cacacatttt gatgcaacca actacgatag actcgtaaag
    23221 ctaagtctga atagtaatac tcttgagtca ataaattttc ctcaaggcag aaatgtaagt
    23281 attacacata tatctatgaa taataatgct ctcagaaata ttgatataga taggctttca
    23341 tcagttactt attttagtgc ggcacataat caactagagt ttgtgcaatt agaatcttgc
    23401 gaatgtctgc aatacctgaa tctcagccat aatcaattat ctgatattgt tgcaggtaat
//
LOCUS       DAZKHC020000069        23272 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZKHC020000069 DAZKHC020000000
VERSION     DAZKHC020000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.069"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(406..717)
                     /locus_tag="ACHOSM_RS15855"
                     /old_locus_tag="pgaptmp_003171"
     CDS             complement(406..717)
                     /locus_tag="ACHOSM_RS15855"
                     /old_locus_tag="pgaptmp_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HGU4559640.1"
                     /translation="MEEDISNAMEQLLDLTISLQTRINQAYSDLCDYTLSQCVISLMK
                     VNVSLDDFISLVSDLELIEDDAKMCTQTITDTASTLKAASEEIQQLVDDLAKSAIPSK
                     W"
     gene            complement(717..920)
                     /locus_tag="ACHOSM_RS15860"
                     /old_locus_tag="pgaptmp_003172"
     CDS             complement(717..920)
                     /locus_tag="ACHOSM_RS15860"
                     /old_locus_tag="pgaptmp_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004012243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HGU4559641.1"
                     /translation="MIGYIQETQLFQMILSDAKNYAELIRLAKETEDYEALERYRDEV
                     LTLKTPFNIVLNMINDALQGEAA"
     gene            complement(978..1361)
                     /locus_tag="ACHOSM_RS15865"
                     /old_locus_tag="pgaptmp_003173"
     CDS             complement(978..1361)
                     /locus_tag="ACHOSM_RS15865"
                     /old_locus_tag="pgaptmp_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559642.1"
                     /translation="MGRKKKNNLKRIEKNSNFNSTRQRPPTHTNNEIPGEEIIFILET
                     ESPELSDEEQEIIKSMPGVYFRPRTRKQTIISDGTLKRWQLLGFSSYHHFLGFNAFNN
                     CVGGASEVDLMAHLEDTLASNKRSN"
     gene            complement(1458..1727)
                     /locus_tag="ACHOSM_RS15870"
                     /old_locus_tag="pgaptmp_003174"
     CDS             complement(1458..1727)
                     /locus_tag="ACHOSM_RS15870"
                     /old_locus_tag="pgaptmp_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559643.1"
                     /translation="MTYNITIGNKTIEITESGYNILKAILDIEFSPPTVVSFCSLGGY
                     SAQHVNHWLNHFTSFGVLDYEGINHTTFRLLKLNKDFELFITNNQ"
     gene            complement(1737..2306)
                     /locus_tag="ACHOSM_RS15875"
                     /old_locus_tag="pgaptmp_003175"
     CDS             complement(1737..2306)
                     /locus_tag="ACHOSM_RS15875"
                     /old_locus_tag="pgaptmp_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4559644.1"
                     /translation="MINEEQFVKVPMWILKISQVNGHKFTDSAKNLYCYLRGFDRAFP
                     SYAKIGDVFGITPRAAEERVKKLLEMGLIIKDARPGSSNLYRVLDIPKGESSLPDPSE
                     KEDESNEILNVSEIIVEEETQESEFPERPEIFTWLLSLDKKKPPHEETEDFVKRALDS
                     KNIYLEKQAFHDLIISLREKSEIYDSIPF"
     gene            complement(2360..2530)
                     /locus_tag="ACHOSM_RS15880"
                     /old_locus_tag="pgaptmp_003176"
     CDS             complement(2360..2530)
                     /locus_tag="ACHOSM_RS15880"
                     /old_locus_tag="pgaptmp_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559645.1"
                     /translation="MSETTEKKRQPRTCFHIPDEVFQVLFKEARSKRITVTDYILDML
                     KREAETLSNKSK"
     gene            complement(2550..2750)
                     /locus_tag="ACHOSM_RS15885"
                     /old_locus_tag="pgaptmp_003177"
     CDS             complement(2550..2750)
                     /locus_tag="ACHOSM_RS15885"
                     /old_locus_tag="pgaptmp_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559646.1"
                     /translation="MKYYTPQDVIEAWKRGEINRFKVRMNRNTARRCGYPEREKCFDD
                     ALKIIDELRKNEKGSEKESETE"
     gene            4252..4803
                     /locus_tag="ACHOSM_RS15890"
                     /old_locus_tag="pgaptmp_003178"
     CDS             4252..4803
                     /locus_tag="ACHOSM_RS15890"
                     /old_locus_tag="pgaptmp_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4559647.1"
                     /translation="MANIGYIRVSTVQQNTDRQLAGVTLDKVFEDKASGKNTQRPQFE
                     AMMNYVREGDVLHVHSIDRLCRNTADLLATVEQLTERGVSVHFHKEDFKTGKNSPAGN
                     MMLTVLAAVAQMEREMMLERQREGIAAAKAAGRIAKRGNGKAIDRAGIVAALANGGSI
                     RSVAKDFSVSTQTVQRIKKEQEA"
     gene            5456..5776
                     /gene="yecR"
                     /locus_tag="ACHOSM_RS15895"
                     /old_locus_tag="pgaptmp_003179"
     CDS             5456..5776
                     /gene="yecR"
                     /locus_tag="ACHOSM_RS15895"
                     /old_locus_tag="pgaptmp_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014885115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR family lipoprotein"
                     /protein_id="HGU4559648.1"
                     /translation="MRKSLMLLLASTLIISGCATKKQLVPTGGSKSDGTVRMSYSYGM
                     FESPVVDPQQGMNAAKARCAAWGYSGAEPFGGFTSQCSQPSSSGCMQTLVTIEYQCTG
                     DIKK"
     gene            complement(5810..6067)
                     /locus_tag="ACHOSM_RS15900"
                     /old_locus_tag="pgaptmp_003180"
     CDS             complement(5810..6067)
                     /locus_tag="ACHOSM_RS15900"
                     /old_locus_tag="pgaptmp_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559649.1"
                     /translation="MVSIVYNNLTIPLHEVDMTVSYKITYEHRDGDQIRMEEVLFHSE
                     HEPTKDEIHDAVRKDTVRFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(6514..6587)
                     /locus_tag="ACHOSM_RS15905"
                     /old_locus_tag="pgaptmp_003181"
     tRNA            complement(6514..6587)
                     /locus_tag="ACHOSM_RS15905"
                     /old_locus_tag="pgaptmp_003181"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6553..6555),aa:Gly,seq:ccc)
     gene            complement(6666..7421)
                     /gene="actS"
                     /locus_tag="ACHOSM_RS15910"
                     /old_locus_tag="pgaptmp_003182"
     CDS             complement(6666..7421)
                     /gene="actS"
                     /locus_tag="ACHOSM_RS15910"
                     /old_locus_tag="pgaptmp_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HGU4559650.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            7839..10111
                     /gene="xdhA"
                     /locus_tag="ACHOSM_RS15915"
                     /old_locus_tag="pgaptmp_003183"
                     /pseudo
     CDS             7839..10111
                     /gene="xdhA"
                     /locus_tag="ACHOSM_RS15915"
                     /old_locus_tag="pgaptmp_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            10122..11000
                     /gene="xdhB"
                     /locus_tag="ACHOSM_RS15920"
                     /old_locus_tag="pgaptmp_003184"
     CDS             10122..11000
                     /gene="xdhB"
                     /locus_tag="ACHOSM_RS15920"
                     /old_locus_tag="pgaptmp_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HGU4559651.1"
                     /translation="MFDFASYHRAATLADAINLLTDNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            10997..11476
                     /gene="xdhC"
                     /locus_tag="ACHOSM_RS15925"
                     /old_locus_tag="pgaptmp_003185"
     CDS             10997..11476
                     /gene="xdhC"
                     /locus_tag="ACHOSM_RS15925"
                     /old_locus_tag="pgaptmp_003185"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HGU4559652.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(11516..13294)
                     /gene="ygeV"
                     /locus_tag="ACHOSM_RS15930"
                     /old_locus_tag="pgaptmp_003186"
     CDS             complement(11516..13294)
                     /gene="ygeV"
                     /locus_tag="ACHOSM_RS15930"
                     /old_locus_tag="pgaptmp_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGU4559653.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRIQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            13773..14960
                     /gene="ygeW"
                     /locus_tag="ACHOSM_RS15935"
                     /old_locus_tag="pgaptmp_003187"
     CDS             13773..14960
                     /gene="ygeW"
                     /locus_tag="ACHOSM_RS15935"
                     /old_locus_tag="pgaptmp_003187"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HGU4559654.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            15018..16214
                     /gene="dpaL"
                     /locus_tag="ACHOSM_RS15940"
                     /old_locus_tag="pgaptmp_003188"
     CDS             15018..16214
                     /gene="dpaL"
                     /locus_tag="ACHOSM_RS15940"
                     /old_locus_tag="pgaptmp_003188"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HGU4559655.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            16272..17483
                     /gene="ygeY"
                     /locus_tag="ACHOSM_RS15945"
                     /old_locus_tag="pgaptmp_003189"
     CDS             16272..17483
                     /gene="ygeY"
                     /locus_tag="ACHOSM_RS15945"
                     /old_locus_tag="pgaptmp_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HGU4559656.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            17536..18921
                     /gene="hyuA"
                     /locus_tag="ACHOSM_RS15950"
                     /old_locus_tag="pgaptmp_003190"
     CDS             17536..18921
                     /gene="hyuA"
                     /locus_tag="ACHOSM_RS15950"
                     /old_locus_tag="pgaptmp_003190"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HGU4559657.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISNGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFAGKAGRGRF
                     LRRKPFVPPVL"
     gene            18969..19901
                     /gene="arcC"
                     /locus_tag="ACHOSM_RS15955"
                     /old_locus_tag="pgaptmp_003191"
     CDS             18969..19901
                     /gene="arcC"
                     /locus_tag="ACHOSM_RS15955"
                     /old_locus_tag="pgaptmp_003191"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU4559658.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIALGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(20122..21747)
                     /gene="yqeB"
                     /locus_tag="ACHOSM_RS15960"
                     /old_locus_tag="pgaptmp_003192"
     CDS             complement(20122..21747)
                     /gene="yqeB"
                     /locus_tag="ACHOSM_RS15960"
                     /old_locus_tag="pgaptmp_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HGU4559659.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGPWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(21795..22565)
                     /gene="yqeC"
                     /locus_tag="ACHOSM_RS15965"
                     /old_locus_tag="pgaptmp_003193"
     CDS             complement(21795..22565)
                     /gene="yqeC"
                     /locus_tag="ACHOSM_RS15965"
                     /old_locus_tag="pgaptmp_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HGU4559660.1"
                     /translation="MKSIIDPSALVMDLGAQKRPTVISVVGAGGKTSLLFWLTELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            22668..23246
                     /gene="mocA"
                     /locus_tag="ACHOSM_RS15970"
                     /old_locus_tag="pgaptmp_003194"
     CDS             22668..23246
                     /gene="mocA"
                     /locus_tag="ACHOSM_RS15970"
                     /old_locus_tag="pgaptmp_003194"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HGU4559661.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPNYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
BASE COUNT         6266 a         5600 c         5256 g         6150 t
ORIGIN      
        1 tgatgttagt aaatagttat aaaatataac tatcagtgta aggctcttta atgttttcgg
       61 acaaaataag gagctttagg ataagcatag atgaaaaata agagcgggaa gggcaaggag
      121 ataacaccta cccgctttta tcaaaattaa gcacaatgcc taacaacatt catcaacaca
      181 acgtagaaac aatcaacaat caaagaggga gttatgatta ttctaaaact tcttgagaga
      241 tggaaggtaa tatttgttag aggttaatgc cactcaaccc ttgaggctat caccttccat
      301 atgcagggat ttatgacagt agaaggatta acaccagtca aaaccttttt atctaccctt
      361 aatactaata taacattttt attatataaa tgcaaatgat tttcattacc atttagaggg
      421 aatagcactt tttgctaaat catctactaa ttgctgaatc tcttcacttg cagcctttaa
      481 cgtgctggcg gtgtctgtga tggtttgagt gcacatcttg gcatcgtcct caattagctc
      541 taaatcgctc accagagaga taaaatcgtc aaggcttaca ttcaccttca ttaagctaat
      601 aacgcattga gagagcgtgt aatcgcataa atctgaatag gcttgattta ttcttgtttg
      661 tagtgagata gtcagatcta aaagttgttc cattgcattg ctaatatctt cttccattat
      721 gcggcttctc cttgtaatgc atcattaatc atattcaata cgatgttaaa aggtgtcttg
      781 agcgtcagca cttcatcacg ataacgctct aatgcttcgt agtcttctgt ttcttttgcc
      841 agtcggatta actcagcata gttttttgca tcagacagga tcatctgaaa taattgtgtt
      901 tcttgaatat agccaatcat tatttttcct ttttcgaata ttttcattgg gcgcttatac
      961 atacgaccat ccttttatta gttagagcgt ttgttagaag ctaatgtatc ttctaaatga
     1021 gccatcagat cgacttccga tgcaccacct acacaattat tgaaagcgtt aaatcccaaa
     1081 aaatgatgat aggagctaaa ccctaaaagc tgccaacgtt tcagagtgcc atcagaaatt
     1141 attgtttgtt ttcttgttct tgggcgaaag taaacgcctg gcattgattt gattatttct
     1201 tgttcttcat cgcttaattc aggggattct gtttcaagaa taaatattat ctcttcccct
     1261 ggaatttcgt tattggtgtg tgtcggtgga cgttgtcttg ttgaattgaa gttactgttt
     1321 ttttcaatac gttttaagtt gttctttttc tttcttccca ttttccttct ttttatgtgg
     1381 attataaaaa cttgatacca ttgttacgat gaatatcgcc ataaatgcat ttgccatatg
     1441 tattgcccga tctgttgtta ttgattgttt gtgataaaca attcaaaatc tttgtttaat
     1501 ttcaaaagac gaaatgtagt gtggttaatc ccttcataat ccagcacacc gaacgatgta
     1561 aaatggttta accaatgatt cacatgttgt gcgctgtagc cgcctaaact acaaaagcta
     1621 accactgtcg ggggtgagaa ctctatgtcc aaaatagctt ttaaaatgtt gtatccgctt
     1681 tcagttattt caatagtttt gtttcctata gtgatgttat aagtcattta ttctccttag
     1741 aatggtattg aatcgtatat ttcacttttt tctcttagag atattatcag atcgtgaaaa
     1801 gcctgtttct caagatagat attctttgaa tctaatgcac gttttacaaa atcttctgtt
     1861 tcttcgtgcg gtggtttctt cttatcaagg gaaagaagcc aagtaaaaat ttcgggccgc
     1921 tcagggaact ctgattcctg tgtttcttct tcaacgatga tttcggaaac attcaatatt
     1981 tcattagatt cgtcttcttt ctcagaagga tcaggtagtg atgattctcc ttttggaata
     2041 tcaagcactc gataaaggtt agagcttcct ggtcgtgcat cttttataat taatcccatt
     2101 tcgagcaatt tcttaaccct ttcttctgct gctcttggtg ttatgccaaa aacatcgcct
     2161 atcttagcgt aagagggaaa tgcacgatca aaccctctca aatagcagta aaggttttta
     2221 gcactatctg taaatttatg cccgtttact tggcttattt ttagaatcca cataggaact
     2281 tttacaaatt gttcttcatt aatcattaaa aatctccttg atgtaatcac gctctacggc
     2341 gctactatgg tgataaaaat cacttgcttt tgtttgagag tgtttcagcc tctcttttca
     2401 acatatcaag aatgtaatct gtaacagtga tacgtttact tctggcttct ttaaacaaca
     2461 cctgaaatac ttcatcagga atatgaaagc aagtgcgtgg ttgtcttttc ttttctgtag
     2521 tttcgctcat agttttctcc ttctcttctt tactctgttt cagattcttt ttcagatcct
     2581 ttttcatttt ttcttaattc atcaataatt tttagtgcat cgtcaaagca tttttcacgc
     2641 tctggataac cacagcgtcg cgctgtgttc ctgttcatcc ttactttaaa acgattgatc
     2701 tcaccacgtt tccaggcttc gattacatcc tgtggagtgt aatacttcat taattaacct
     2761 cctattctat ttggttaaaa gaaaaagaca ccttgcaagc cttgggcttc aaagtgcggt
     2821 gcattggttt aattacttag aggataagaa tgttttctgt ccctaatatt actttaccac
     2881 gactaaaagc ttttgtcaac ccctatccat ataaatattg atgattttca gtaagttatg
     2941 atatttctta gcaattcgtg ctctcatgtg tagtgctatt ctttattatt gtctaaaacg
     3001 ctctaaaacg cgttctaacg aggttttgat aaaaacaata cattccctta tgtaatggct
     3061 gtttagctcg cctacggctt gatttaagcg gtagaatctt atttaacctt caactactaa
     3121 aaccattctt ttttgtttgt tgttggttgt tgtctgtgtc tatgtgctaa catcgctaac
     3181 gcgtagcaca caaggttatt gcactcgtca gagtattgca caatagattt tctcttaaat
     3241 agattgatga tcgttagatg gtagttagtt aattatcgtt ctgcgcacag cgcaagacta
     3301 tttagcctta cgcaagccgt aggctatttg cacatagtag attacatatt cgttgtgagc
     3361 ggcattagcg aacatagatc tactaactaa catcaacaaa caaaaaaata tgtgttatga
     3421 caagcagtct aacgactaac actctaactt gtgcaaggtt ctggcgaatg ccaatagatt
     3481 aagggatagt cgaagactcc cctaacgggt atccctataa ttattcttta aagataaaag
     3541 aatttataaa agataattta attgtagttt gatagttgat ctaataggta acaaaattct
     3601 gtaggggtgg ctaacagttt tctgtggggg tgtctaacat aattctgtta gggttggggt
     3661 aacagttttc tgttagggtg catatttctt taaaagttaa ggataacaga attctgtggg
     3721 gggtagttca cagtgaccta ccgttacatc catttatcac ataacatctg tgcaatgatc
     3781 ttctaaaagt gtttcttgct atcaaaaatt aaacatctaa aacaggctta aattcaagat
     3841 taattgatga ttaatcccta tgtaattcca tactaaaaat tcaatgaaaa gtagcttaaa
     3901 agtgcaatgt tagctttctt cacttttaca tcactttcgc tgcgccttgt gtaatgtggc
     3961 ttacagagca ctatcagaaa ttgttgatta aaaatcaatc tattatttgt cgaacaagtt
     4021 cctttcacgt gttgaaaaaa ttaatttttt caaaataaac acgctattta atagcttgct
     4081 aattttacca aaacataact acatgattct catgcaagac atgtagtgat aaagcaggct
     4141 aaatcaccac actaaaaacc ttcgaatttc gtagtgaaaa aagtccttta cacacctaat
     4201 caacacgatc aataatcact acacacaaat caacacagaa ggaaaatgaa aatggctaat
     4261 attggataca ttagggtatc gactgtccaa caaaacacag accgtcaatt agctggagta
     4321 actcttgata aagtctttga agataaagcg tcaggcaaga atacccagcg cccacagttt
     4381 gaggcaatga tgaactatgt gcgtgaaggt gatgttttgc atgttcacag cattgatcga
     4441 ctttgccgta atacagccga cctactggca acggttgagc aattaacaga gcgcggcgtg
     4501 tctgtgcatt tccataaaga agactttaag acagggaaga actctcccgc agggaatatg
     4561 atgctcacag tgcttgcagc agtggcacaa atggagcgtg aaatgatgct tgagcgtcaa
     4621 cgtgaaggga tcgccgcagc taaggccgct gggcgtatag caaaacgtgg taatggaaaa
     4681 gcaattgatc gagcgggaat tgttgccgct ctcgcgaatg gtggatcaat tcggagcgta
     4741 gcaaaagatt tcagtgtcag cacacagacg gtgcaacgta tcaagaaaga gcaggaagcg
     4801 taatgcctcc tgatatttca gaattgtcgc tgcatggacc tgacggcgct gatcttctcg
     4861 ttaagcgatg atcacgtatg cgccgatttc agagaaaaag acagtaactg aactcactat
     4921 gaaggctaaa cgtatcttta cacttgatgg aaacgtccaa aaaacaacct aaccccaaaa
     4981 cccaacaaat tgcaagatat tgtttttaaa agaaaatcaa atctacatca aatattggtt
     5041 gacgtagatt gaggtgaata gtagactatt aggataaatt accagtattg cacacaacaa
     5101 atttgcatga tttaatcccc ctcacattca tttagggggc gttatgcatt tatcttcaaa
     5161 acatcttagc actatccgct ttctcgttat tgcttcattc gttgtagcta tcagttaccg
     5221 actttttgtt gcctccaatg acgaaatgaa tcctcttgct attattgccc attcaatagc
     5281 gacaatagtt agacggtttg aaaaaccgta atacaataaa catccgaaat aatttcgaaa
     5341 gcaactcatt tttactctgc aaacaatcag aaaagactta attttgctaa gttaatgcat
     5401 taatctaaaa acgctttaaa tcaatacata accattatct cttgggatta tttacatgcg
     5461 caaaagttta atgctgttac ttgcttcaac attgattatt tctggatgtg caacaaaaaa
     5521 acaactcgtt ccaactggtg gaagcaaatc agacggcaca gttaggatga gttattctta
     5581 cggtatgttt gaaagtcctg ttgtagatcc acaacaaggc atgaatgcag ccaaagcgcg
     5641 ttgtgccgct tggggatatt caggagccga gccttttggt ggattcactt ctcaatgttc
     5701 acaaccttct tcatctggtt gtatgcaaac acttgtgact atcgaatatc aatgcactgg
     5761 cgatattaag aagtaataat taggggcttc cagccccttc tataaccatt catggaacag
     5821 ggacaaccga aatcacttta aaccctgaaa cagccgttac tccattagat ggagaattaa
     5881 atctcacagt atcttttctt actgcatcat gaatttcatc ttttgttggt tcatgctctg
     5941 aatggaataa aacctcttcc attctaattt gatcaccgtc tctatgctca taagtaatct
     6001 tgtagctaac agtcatatca acctcatgca ggggaattgt gagattatta tagacaatag
     6061 aaaccataaa gccccgttgg tgatacagca acaagcggga gcgtagcgac ttcatcagat
     6121 tgtcagaaag atcacacctt atcaggataa ccgtgtaccc gttgggagcg tggctgaaac
     6181 gctgatagag cttgcatatg gtttgcaagg accaatatca ccataaacct gtttacgcag
     6241 ccacaaggcc gcacatcggg cttaaatgcg tgtgttcagt ctgtcgatga cattacaatg
     6301 ccaggatggg ctttatcttc acgttgtgct atcgcggtgt gagaggtgca actgccgcca
     6361 acgttaggtt atactgtcat ctcaccgtgt aacttaacgt gacagttact aagggcggat
     6421 cacaatggta gaactcctaa gcaaattaaa taggtaactg tttaagttgc tgacagagaa
     6481 gactttttta gtcttctcca cctggggaga ttctggagcg ggcgaaggga atcgaaccct
     6541 cgtatagagc ttgggaagct ctcgttctac cattgaacta cgcccgcttc gagatgcgta
     6601 aggcattata aaccttacgc tctccttagc aagtgccgcg ctgctgactg ctgattaatt
     6661 cgccatcagc attttggctt gctgccctgc ggcggcaagt aacgtagcgg atcaattgcc
     6721 gttgcacggt aacgaatctg gaaatgcagg cgaacagatg ccgcatccgt gctccccata
     6781 gtggcgattt tttgcccagc cttcacgctt tgcccattat ttaccagcat cgtgtcatta
     6841 tgggcgtaag ccgtaatgta atcttcactg tgtttaatca tgatgagatt accgtagcca
     6901 cgcagctggt tgcccacata caccaccttt cctgcacccg cggcgtaaat aggtgtaccc
     6961 cgtggagctg agatatcaat ccctttattg ccgccatctg ctgtcgaata cggcatgata
     7021 actttccctg tcgttggcca taaccaacaa cgttgcccta ctggcggcca ggaagatttc
     7081 ggtaccgctg atgacggtgt aaccgatgcg gttttggtcg ttgatttggc ggttgattta
     7141 cgtgtactac tactactttt cgccccaccc agttttagtt tctgaccaac ttcaatggtg
     7201 taaggggggg aaatgccgtt cagtcgcgcc agctctttta cgctggttcc cgtggtgcgc
     7261 gaaatacgat atagcgtatc cccccgtttc acggtgtaaa cggagccgga atacgttcct
     7321 gtatcggatg atttgctacc cgaacagccc gccaaaagca gtccaaccga taacaacatc
     7381 acgataccca gagatttttt attcaggcgt cccgcactca aaatctttcc tcgcttaaaa
     7441 aacaagacgc catatcatag cagccagcct cttgataccc aatctcttgc ttctggaaaa
     7501 acgtggtaat agagaaagag gttaatttca tttggcaaag ggaagtatca tcagcaaaat
     7561 attcgctgta gaggtatcga agttcctgac tggattttac cctatttccc atcctgatgc
     7621 gtaataatcc tgatttatca cattgatttt atcgccactc atattgatac gtatcactgt
     7681 ccctattcag agtcctgttt cgtatcatat ctgtgttttt ttaacgtcat cacacttcct
     7741 tcctccccta acgcttatcg tcgtttctgg cgtaaatctt gcctgcttag actaaatctt
     7801 tgccataaga accaaatgtt caaatcgagg ggatttctat ggaagcgcgg gaagcaaccg
     7861 ctacgggtga atcatgcatg cgcgtcgatg ccattgctaa ggtcaccggg cgggcacgat
     7921 atactgacga ttatgttatg gcgggcatgt gttacgcgaa atatgtacgt agccctatcg
     7981 cacatggtta tgccgtaagt attaatgatg aacaagccag aagtttaccg ggcgtactgg
     8041 cgatttttac ctgggaagat gtgcctgata ttccattcgc tacagctggg catgcctgga
     8101 cacttgacga aaacaagcgc gataccgccg atcgcgcact gctaactcgc catgttcgtc
     8161 atcatggtga cgccgttgcc atcgtcgtgg cccgcgatga actcacggca gaaaaagcgg
     8221 cgcaattggt cagcattgag tggcaagaat tacccgttat caccacgcca gaagcggcgc
     8281 tggcagaaga cgctgcacca atccataacg gcggcaattt actgaaacaa agcacgatgt
     8341 cgacgggcaa tgtccaacaa acaatcgatg ccgccgacta ccaggtacag gggcactatc
     8401 agacccccgt tattcaacat tgtcacatgg aaagcgtaac atcgctggcg tggatggagg
     8461 atgactcgcg aattaccatc gtttccagca cccagatccc gcacattgtt cgccgcgtgg
     8521 ttggtcaggc gctggatatt ccctggtcat gcgtacgagt catcaaacca tttgtcggtg
     8581 gcggttttgg taataaacag gatgtactgg aagagccaat ggcggcattc cggtgaaagt
     8641 ttcccttagc cgtgaagagt gtttcctcgc aacccgtacc cgccacgctt ttaccattga
     8701 cgggcaaatg ggcgtgaacc gcgacggaac attgaaaggt tatagtctgg atgttctgtc
     8761 taacaccggc gcttatgcat ctcacgggca ctccatcgct tctgcggggg ggaataaagt
     8821 cgcttacctt tatcctcgtt gtgcctacgc ttacagttca aagacctgct ataccaacct
     8881 cccctcggct ggtgcgatgc gtggttattg cgcgccacaa gtcgtatttg ccgttgagtc
     8941 tatgcttgat gacgccgcga cagcgttagg tattgatcct gttgaaattc gtttacgcaa
     9001 cgccgcccgc gaaggagatg ctaatccgct cacgggcaaa cgtatttaca gcgcagggtt
     9061 gccggagtgt cttgaaaaag gccggaaaat ctttgaatgg gaaaaacgcc gtgcagaatg
     9121 ccagaaccag caaggcaatt tgcgccgcgg cgttggcgtc gcctgtttta gctacacctc
     9181 taacacctgg cctgtcggcg tagaaatagc aggcgcgcgc cttctgatga atcaggatgg
     9241 aaccatcaac gtgcaaagcg gcgcgacgga aatcggtcag ggtgccgaca ccgtcttctc
     9301 gcaaatggtg gcagaaaccg tgggggttcc ggtcagcgac gttcgcgtta tttcaactca
     9361 agataccgac gttacgccgt tcgatcccgg cgcatttgcc tcacgccaga gctatgttgc
     9421 cgcgcctgcg ctgcgcagtg cggcactatt attaaaagag aaaatcatcg ctcacgccgc
     9481 agtcatgcta catcagtcag cgatgaatct gaccctgata aaaggccata tcgtgctggt
     9541 tgaacgaccg gaagagccgt taatgtcgtt aaaagatttg gcgatggacg ctttctacca
     9601 ccctgaacgc ggcgggcagc tctctgctga aagctccatc aaaaccacca ctaacccacc
     9661 ggcgtttggc tgtacctttg ttgatctgac ggtcgatatt gcgctgtgca aagtcaccat
     9721 caaccgcatc ctcaacgttc atgattcagg gcatattctt aatccactgc tggcagaagg
     9781 tcaggtacac ggcggaatgg gaatgggcat tggctgggcg ctatttgaag agatgatcat
     9841 cgatgctaaa agcggcgtgg tccgtaaccc caatctgctg gattacaaaa tgccgaccat
     9901 gccggatctg ccacaactgg aaagcgcgtt cgtcgaaatc aatgagccgc aatccgcata
     9961 cggacataag tcactgggtg agccaccaat aattcctgtt gccgctgcta ttcgtaacgc
    10021 ggtgaagatg gctaccggtg ttgcaatcaa tacactgccg ctgacgccaa aacggttata
    10081 tgaagagttc catctggcag gattgatttg aggataacat catgtttgat tttgcttctt
    10141 accatcgcgc agcaaccctt gccgatgcca tcaacctgct gactgacaac ccgcaggcca
    10201 aactgctcgc cggtggcact gacgtactga ttcagctcca ccatcacaat gaccgttatc
    10261 gccatattgt tgatattcat aatctggcgg agctgcgggg aattacgctg gcggaagatg
    10321 gctcgctacg tatcggctct gcaacgacat ttacccagct aatagaagat cctataactc
    10381 aacgtcatct cccggcgtta tgtgctgcgg ccacgtccat tgctggaccg cagatccgta
    10441 acgtcgctac ctacggtgga aatatttgca acggtgccac cagcgcagat tctgccacgc
    10501 caacgctaat ttatgacgcg aaactggaga tccactcccc gcgcggtgtt cgtttcgtcc
    10561 cgattaatgg ctttcacacc gggccgggca aagtgtctct tgagcatgac gaaatcctcg
    10621 tcgcctttca ttttccgcca cagccgaaag aacacgcggg cagcgcgcat tttaaatatg
    10681 ccatgcgcga cgcaatggat atttcaacga ttggctgcgc cgcacattgc cgactggata
    10741 acggcaattt cagcgaatta cgcctggcat ttggtgttgc cgcgccaacg ccgattcgct
    10801 gccaacatgc cgaacagact gcacaaaatg cgccattaaa cctgcaaacg ctggaagcta
    10861 tcagcgaatc tgtcctgcaa gatgtcgccc cgcgttcttc atggcgggcc agtaaagagt
    10921 ttcgtctgca tctcatccag acgatgacca aaaaagtgat tagcgaagcc gtcgccgcgg
    10981 cggggggaaa attgcaatga atcacagcga aacaattacc atcgaatgca ccattaacgg
    11041 gatgcctttt cagcttcacg ccgcaccagg cacgccgctc tcggaattac tccgcgaaca
    11101 aggactgcta agtgtcaaac aagggtgctg cgtgggtgaa tgtggtgcct gtacggtgtt
    11161 ggtcgacggc acagcaatag acagttgctt ataccttgcc gcctgggctg aaggaaaaga
    11221 gatccgcacg ctggaaggtg aagcgaaagg cggaaaactt tctcatgttc agcaggctta
    11281 tgcgaaatcc ggcgcagtgc agtgcgggtt ttgtacgcct ggcctgatta tggctaccac
    11341 ggcaatgctg gcgaaaccac gcgagaagcc attaaccatt acggaaattc gtcgcggact
    11401 ggcgggaaat ctttgtcgct gcacggggta tcagatgatt gtaaatacag ttctggattg
    11461 cgagaaaacg aagtaaaagg atatccggcc tgaattcagg ccggattcac tgaggttatg
    11521 tgtttaacaa ctcatatttc ttaatcttgc gatagagcgt agcaatgccg atgcccagtt
    11581 catcagcaac ttgcttcttg ctgttatgac gtgaaagcgc ctcgcggatc atttgctttt
    11641 ccatctcctc cagcgccgtg ccgcccgcat catcgagtga caggtgcgcc tcactgacct
    11701 ctgttacatc actttgctcc gttgtgccat tattcagcag atttggcggc aatagcgtgc
    11761 tgtcgataac ttcacctgaa ggaaccacgt taaccagata ttccatcaaa ttgcttaact
    11821 cgcgcaggtt tccgggccaa cgatgcttac gcaatatttc gacgacatcg ggagcaatgc
    11881 caggataaac cgatcccaga cgacgggtat gcagatgtaa aaagtaatgc accaatagtt
    11941 caatatcttc ctgacgttca cgcagcggtg gcagagttat cgggataaca ttaagtcggt
    12001 agaatagatc ttcgcggaat ttaccttcgg caatgaactg ggccaaattc tgattagttg
    12061 cagaaatgat gcgaatgtcg acttgtattg ggctactggc accaatcggc agaatttcac
    12121 gcgcctcaat agcgcgcagt aatttagcct gcaacattaa tggcatatca cctatttcat
    12181 cgagaaacag cgtgcccgta ttcgccgcct gaatcaaccc tgttttaccg ttggcagaag
    12241 cgccagtaaa tgcgccttta acataaccga acagttcgct ttccagaagc tgctccggaa
    12301 tcgcggcaca gttgatagca ataaagggtt tattccgtct tccgctcaac ttatggattg
    12361 ctcgggcgac gacttcttta cccgtgccgc tttcaccaac caccataacg ctggatgggc
    12421 tgggtgcaat acggctaatg agtcgtttta attgccgcat aacacggcac tcgccaacca
    12481 attgttcaat atgtggttca tcaggtgcat ttgctacaga aaaactggta tgcgattggt
    12541 gaaatgccat taaaaataat tgtcggccct gaatgttatg caattgacca atgattaatt
    12601 cacttttatc gtcccacgaa acaatatgct gcatatgtcc atgagtaaaa ttactctcaa
    12661 atgttaatgg tctgaaacgg ataggtttcc caataatatt attttgtaca acaccaagtg
    12721 tttttaaggc agtctgatta acaaactgaa tccgactttc atcatcaaca actaatacgc
    12781 cctgatccat attatcgatc atggtcgcga atattttact gatgttatct cctggcccct
    12841 gatcctccag aagtttcgaa acaaaaatgg tggatatatg gcgaacataa tcagaaaatt
    12901 cgcgtaaatt atcactgata tgctcttgtt gctcgtgggt aacggcaatc aaacttatca
    12961 ccccaacaca acgatcctgt aaaatgacag gcgtacccag aaatgctttt tcgcggcaat
    13021 tttctttact atcgcaacct tcgcaaaggg gatcgaagcg agactgtgtc acaacttttt
    13081 cagttttcgt ttccaggacg tggcggagca ggcgtgagtt gccgctcaac tggcgaccaa
    13141 gaaacttccc atacgcgccc gttccggcga cgcgacacaa gttttcatca acgatctcaa
    13201 cctcaagctg caaaacgctg gcaagcattc tggcaaaacg ctgaattgtc ggttgaattt
    13261 gcatcaatac tgactgcgta gtagcaagct ccatagcttt accttccaga cttacttaaa
    13321 agtcgatcat tgaagacgtt gatggttcac agatcatgat gatattaact caggcgaaat
    13381 tggctttgat aaaaacataa gatttttatc attttctaat gaaattatgg aagagatatc
    13441 acatttctat atcaatatga gaattacggc ggtgagttta tcaaactgaa gagagatagc
    13501 ctgccccttt atcttatttc tgatacttag cagcaaataa ataacgcgat aaaaaaagcc
    13561 aaacgttttc gtattttaca aacaaccaga agctggcatc aatttgtgat caaccccaca
    13621 cattatccgt caaattagtc ttttgcagcc gcgcggataa ttctggcaca cttattgtta
    13681 gtcccaagta tagctgtgaa aacaccaatc actttggcaa gtcacagtga aataaaccac
    13741 tttgcctgtc attccactac tgggacttta tgatgaaaac tgttaatgag ctgattaagg
    13801 atatcaattc gctgacctct caccttcacg agaaagattt tttgttaacg tgggaacaga
    13861 cgccagatga actgaaacaa gtactggacg ttgccgcagc attaaaagca ctgcgtgctg
    13921 aaaacatctc aaccaaagtc tttaatagtg gattaggtat ttccgtattc cgcgacaact
    13981 ccacccgtac ccgcttctct tatgcttccg cgcttaacct gctcggcctt gcacaacaag
    14041 atctcgacga aggcaaatca caaatcgctc acggcgaaac cgtgcgtgaa accgccaata
    14101 tgatctcctt ctgcgccgac gctattggta ttcgcgacga tatgtatctg ggcgcaggca
    14161 acgcctatat gcgtgaagtt ggcgctgcac ttgatgacgg ttacaagcag ggtgtactgc
    14221 cacagcgtcc ggctttagtg aacctgcaat gcgatattga ccacccgact cagtcaatgg
    14281 ctgacctcgc gtggttacgt gaacactttg gttcactgga aaacctgaaa ggtaaaaaaa
    14341 tcgccatgac ctgggcctac tctccaagct atggcaaacc gctctctgta ccacaaggca
    14401 tcatcggtct gatgactcgc ttcggtatgg atgtcaccct ggcccatccg gaaggctacg
    14461 acctgatccc ggatgtggtt gaagtggcga aaaacaatgc taaagcctcc ggtggtagct
    14521 tccgtcaggt caccagcatg gaagaagcct tcaaagacgc agacatcgtt tatccgaagt
    14581 catgggcacc ttacaaagtg atggaagagc gtactgaatt gctgcgtgcg aacgatcacg
    14641 aaggcttaaa agcactggaa aaacagtgtc tggcacagaa cgcgcaacac aaagactggc
    14701 attgtactga agagatgatg gaactgaccc gtgatggcga agccctgtac atgcactgcc
    14761 tgccagctga tatcagcggc gtatcctgta aagaaggtga agtgactgaa ggcgtattcg
    14821 aaaaataccg tatcgctacc tacaaagaag ccagctggaa gccttatatc atcgccgcga
    14881 tgatcctgtc ccgtaaatac gccaaaccag gtgcactgct cgagcaactg ctgaaagaag
    14941 cgcaagaacg cgtgaaataa cccctcgggc cgatcagctg gtcggccctc tatttccaga
    15001 ggccaaaagg ataggatatg tccgttttct cattgaagat tgatatcgcc gataacaaat
    15061 ttttcaacgg cgaaacatca ccgctctttt cgcaaagcca ggccaaactg gcgcgccagt
    15121 tccaccagaa aatagctggt tatcgcccaa caccgctttg tgcgctggac gatctcgcaa
    15181 acctttttgg tgtgaagaaa attctcgtca aagacgaatc aaaacgattc ggtctgaacg
    15241 ccttcaaaat gcttggcggt gcgtacgcca tcgctcaatt attgtgtgaa aaatatcatc
    15301 ttgatatcga aacgctgtca tttgagcacc tgaaaaatgc catcggcgaa aaaatgactt
    15361 tcgcgaccac caccgacggc aaccacgggc gcggtgtggc gtgggcagca cagcaactcg
    15421 gacagaatgc ggtgatttac atgccgaaag gttctgctca ggaacgcgtt gacgccattc
    15481 tgaacctcgg tgccgagtgc atcgtcacgg atatgaacta tgacgatacc gttcgcctga
    15541 ccatgcaaca cgcgcagcag cacggctggg aagtggtaca ggacacggca tgggaaggtt
    15601 acaccaaaat cccaacctgg atcatgcaag gctacgcaac cctggcagat gaagccgtcg
    15661 agcaaatgcg tgaaatgggc gtaaccccga cgcacgttct gctgcaagcc ggtgtcggag
    15721 caatggccgg tggtgtgctg ggttatctgg tcgacgtcta cagcccgcaa aatctgcaca
    15781 gcattattgt tgaacctgac aaagctgact gtatttatcg ctccggcgtc aaaggcgaca
    15841 tcgtcaacgt tggcggtgat atggccacca tcatggcggg cctggcctgt ggcgaaccta
    15901 acccgctggg ctgggaaatc ctacgtaact gcgccaccca attcatctcc tgccaggaca
    15961 gcgttgccgc attaggtatg cgcgtgctgg gtaatccgta cggcaacgac ccgcgcatca
    16021 tctccggtga atccggcgct gtcggtttgg gcgttctcgc agcggttcat tatcacccgc
    16081 aacgtcaaag cctgatggaa aaactggcgc tgaacaaaga tgccgtagtg ctggttatca
    16141 gtactgaagg cgacaccgac gtgaagcact accgcgaagt tgtctgggaa ggcaaacacg
    16201 ctgtagcacc ttaatcaccc tattggaact actccctgca aaacggggag taaaaaatct
    16261 ggagaaaaat aatggctaag aatattccat tcaaactgat tcttgaaaaa gcaaaagatt
    16321 accaggcgga catgactcgc ttcctgcgcg acatggttgc tattcccagt gaaagctgcg
    16381 acgaaaaacg cgtagtacat cgtattaaag aagagatgga aaaagtcggc ttcgataaag
    16441 ttgaaatcga cccgatgggc aacgttctcg gttatatcgg ccacggcccg cgtctggtgg
    16501 caatggacgc tcatatcgat accgtcggca ttggcaacat caaaaactgg gacttcgatc
    16561 cgtacgaagg catggaaact gatgagctaa tcggtggtcg cggtacttcc gaccaggaag
    16621 gcggcatggc atctatggtt tatgccggta aaatcattaa agacctcggt ctggaagatg
    16681 aatataccct gctggttacc ggtactgtgc aggaagaaga ctgcgacggt ctgtgctggc
    16741 agtacattat tgaacaatcc ggcattcgcc cggaatttgt ggtcagtacc gaaccaaccg
    16801 actgccagat ataccgtggt cagcgcggtc gtatggaaat tcgtattgat gttcagggtg
    16861 ttagctgcca cggttctgcg ccagaacgcg gtgacaacgc cattttcaaa atgggtccga
    16921 ttcttggcga attacaagaa ctctcccaac gtctgggtta tgacgaattc ctcggcaaag
    16981 gcaccctcac cgtttctgaa atcttcttca catccccaag ccgttgcgct gtagcagata
    17041 gctgcgccgt ctctattgac cgccgtctga cctggggcga aacctgggaa ggcgcgctgg
    17101 acgaaatccg cgccctgcct gcagtacaga aagctaacgc ggttgtttct atgtacaact
    17161 acgaccgtcc gtcctggact ggcctggttt acccaaccga atgctacttc ccgacctgga
    17221 aagtggaaga agatcacttc accgttaaag cactggtgaa tgcctacgaa gggctgtttg
    17281 gcaaagcgcc ggttgttgat aagtggacct tctcaactaa cggcgtatct atcatgggcc
    17341 gtcacggcat tccggtgatc ggctttggcc cgggtaaaga acctgaagcg catgcaccta
    17401 acgaaaaaac ctggaaatct cacctggtga cctgtgccgc gatgtacgct gcaatcccgt
    17461 taagctggct ggcaacagag taattactct tcacttattc ccctccggtt cgccggaggg
    17521 tttttggagt ttgctatgcg cgtattgatc aaaaacggca ctgtcgttaa cgcagatgga
    17581 caagccaaac aggatttgct gattgaaagc gggattgttc gccagttggg caacaatatt
    17641 tcgccgcagc tcccgtatga agaaattgat gccactggct gttacgtttt ccctggcggc
    17701 gtggatgtcc atacgcattt caatattgat gtcggcatcg cgcgcagttg tgatgatttt
    17761 tttaccggta cccgcgcagc tgcgtgtggc ggtacaacaa ccattattga ccatatggga
    17821 tttggcccaa acggctgtcg gttacgccat caactggagg tttatcgtgg ttatgccgcc
    17881 cataaagcgg tcatcgatta cagctttcac ggtgtgatcc agcacattaa tcacgcaatc
    17941 ctcgacgaaa tcccgatgat ggtcgaggaa ggactgagca gttttaaact ctatttaacc
    18001 tatcaataca aactcaacga tgacgaggtt ttgcaggcat tacgccgctt gcatgaatcc
    18061 ggcgcgctga ccaccgtgca cccggaaaat gatgcggcta tcgccagcaa gcgggcggaa
    18121 tttatcgccg cagggttaac cgcgccgcgc tatcatgcct tgagtcgccc tctggaatgc
    18181 gaagcggaag ccatcgcccg catgattaac ctggcacaaa ttgccggtaa cgccccgctc
    18241 tatatcgtgc acctgtctaa cggcttaggt ctggattatc tgcgtcttgc ccgtgcgaat
    18301 caccagccag tctgggttga aacctgccca caatatctcc tgttggacga acgcagttac
    18361 gatacagaag atggcatgaa gttcattctt agcccaccgc tgcgtaacgt acgcgagcag
    18421 gacaaactgt ggtgtggcat cagcgatggt gcgattgacg tggtggcgac cgatcactgc
    18481 accttctcga tggctcaacg cctgcaaatt tctaatggcg atttcagtcg ctgcccaaat
    18541 ggcttacccg gtgtggaaaa ccgcatgcag ttactgtttt ccagtggcgt gatgacggga
    18601 cgtataacac cggaacgctt tgttgaatta accagcgcaa tgcccgccag gctgtttggc
    18661 ctgtggccgc aaaaaggatt attagcgccc ggttccgatg gcgacgtggt gattatcgac
    18721 ccacgtcaga gccaacaaat tcagcatcgc catctccacg acaacgccga ctactcgcca
    18781 tgggagggtt ttacctgtca gggcgcgatt gtcagaacct tatcccgtgg tgaaacgatt
    18841 ttctgtgacg gcacctttgc aggcaaagcc gggcgaggtc gtttcctgcg acgcaaaccg
    18901 tttgtccctc ccgtgctcta accactgcca gggatctata cacaatcaag aatgatgagt
    18961 gaggaaaaat gagtaagaaa attgttctcg ccctgggcgg gaatgcgctg ggcgatgacc
    19021 tggccggaca aatgaaagcg gtaaaaatta cttctcaggc aattgttgat ttaattgccc
    19081 tgggacatga agttatcgtc actcatggta acgggccgca ggtgggcatg attaaccagg
    19141 cctttgaagc tgctgcaaaa actgaagcgc actcgccaat gctgccgatg tctgtctgcg
    19201 ttgccttaag ccagggttat attggttacg atctacaaaa cgccttaaga gaagaactgc
    19261 tttctcgggg cattaataaa ccggtagcaa cgctggttac tcaggtcgaa gtcgacgcta
    19321 acgatccggc attcctcaac ccgaccaagc cgatcggttc gttctttacc gagcaagaag
    19381 cagagcaact gacaaaacag ggttacactc tgaaagaaga tgctggtcgc ggctatcgcc
    19441 gtgttgttgc ctcgccaaaa ccggttgata ttattgaaaa agaaacggtt aaagctctgg
    19501 tagatgcagg ccaggtggtg atcaccgttg gcggtggtgg tattcctgtt attcgtgaag
    19561 gtaaccatct gcgtggtgcc agcgcggtta tcgataaaga ctgggccagc gcccgtttag
    19621 cagaaatgat tgatgccgat atgctgatca ttctgactgc ggtagaaaaa gtggccatta
    19681 acttcggtaa agagaatgaa cagtggctcg atcgcctgtc attaagtgat gctgaacgct
    19741 ttatcgaaga aggtcatttt gcgaaaggtt ctatgctgcc taaagtggaa gctgccgcct
    19801 cgtttgctcg ctctcgcgca ggtcgtgaag cactgattac cgtattgagc aaagcgaaag
    19861 aagggattga aggcaaaacc ggaacggtga tttgtcagta attttaaggc cgggggggta
    19921 ataccatccg gccaaagatt gtcaagtaag tgcgctttgt tgatgccgga tgcggcgcga
    19981 gcgccttatc cgtcctacaa aacatcgcaa attcaataga ttgcaatgca cttgtaggcc
    20041 tgataagcgt agcgcatcag gcaatttggc gtttgtcatc agtctcaggc cgggggggta
    20101 ataccatccg gccattttca tttaagccac ttccagcact tcttttgtcg ctttcacgcc
    20161 ctgatgcatc aacatcatta acgcctcaag tacgccgccg ccaatcgccc gggctttatc
    20221 agaaacgctg gtgaaatcag ccgtttcacc acgaggatcg atatcaccaa ttttaaaacc
    20281 accgaccact gccagtccgt cgttcaacaa gccacgcacc atccccgtca acggtgcttt
    20341 aatttcatgc tcaccaatcc aggcaatcac atcgccctct ttcaccagat cgcctaattt
    20401 cacgttggat cgcataatgc ctgcagcagg agcacggatc acccgtcggg tggtatgccc
    20461 cataatattg ccaggaacac cggtattctc ctgcgcacaa ccagagtaaa tcacctgacc
    20521 gagccagggc ccgcgatttg tttcaattac cgcatgacaa tccttcccag cagtaaagcc
    20581 cggcccaagc gcgattgtta ctggtgccat atctgcccgt gttcccaaat tctgtttcgc
    20641 cagaatagcg tccaccacgc aaagcggttt cagttcatca agcaatgaac aggcgggatc
    20701 taccatcaca gggatgaatc cgcgttcggt aagtttcatc gcttccgcag agctggttgc
    20761 caggcgagca gtgacgcctt cgaccgtcat ttcgccatcg aacacggcct gggcaaacgc
    20821 cacggtacaa cgaatcactg tcggtttttc cacttccagc atgatcactt taaaacccgc
    20881 atgatacaga cgtagcgcca caccactggc gatatctccc gcaccgcgaa tcaccaccag
    20941 ctggtgtccg gaaggatgag aaggtttcat catcagccca cccggcgcgt tatttttcac
    21001 ctgtaatatt tctgccagca cgctgatggc gatctcctgc ggcgtttccg cacctatgtt
    21061 gtaaccaacg ggcgcatgta aacgggcaat atgttcttca gccacgcctt tctcacgcaa
    21121 ttgacgcagg aaaagctgaa ccttgcggcg acttgccagc aaccccaacc atgcaatggg
    21181 ctgttcaatg agtttgtcga gggcttcacg atcctgatta ttcgtggcaa tcaggacaaa
    21241 attatcaggg cgaatatcca gtgcttccac tgccgcacca aacgactcag catggagaag
    21301 cgtggttgat ggtgggaata gttcaggatt gaggctttcg cgataaatat cggcaacggc
    21361 aatatcaaat cctaatagcg ccgcactctg ggctatcgcc cggttgacat gccccgcgcc
    21421 aatcaacacc agacgtggac gcataccatg gacgctgata aatactgaca ttgcacctcc
    21481 acaatctgac ccgacagcat ccgcaccgtt acgagccata cgtccatgga ataatcgcgg
    21541 cttacgttcc tgcaatgcct gaagtgactc ttcaatcacc ttccgctcaa ccattccgcc
    21601 accaattgta ccgacgatag aaccatcggc gcgcactaac atttgtgcag aatggcgggg
    21661 agtcgagcct cggctatcaa caatttgcgc catcgcaaac ggacaatttt gctcttcgag
    21721 ttttgcagcc tctgtgaaaa tattcatacc aacctcaatg gatatccttt cagtaacccg
    21781 gaatacccgg gccgctaatt cacaaatctg cgcgcgattg caggatgttc ttgtatatcg
    21841 cccagccaga ttgcctctac gtcgtgttgt tgcagcggtt gaaggagctc gctttgtgca
    21901 atcgcattct cacattgaga aaaacggtta atgaaccaga ctcgccgaca accttgcggt
    21961 acgtttttaa acgccccctg agggtggcga accagcgcaa cgagatcgct cagttgcaag
    22021 gttgcatcag gtgttaaccc ggtaatatca gcaaactgcg accagcgatg gacattttct
    22081 gtgctcactt tcgcgcccaa agtatgtcct cccatcacag caatcacgca gcaactgctt
    22141 ttgggtatgc aaggttcgtg ctcatcaggc gcttttaacg gcattccacg cgagccatct
    22201 gcttcaatga gaattacgtc acactctggt cgttgcacca gtgcatcaat cgcttctggc
    22261 gtaaatccct gcacttttcc ctggttcgct ttccagctgt gaaaacaaaa tgaaatgggg
    22321 gatgtaagag acgcatgagg aagcatggcg ggatcacgac agaaaaccac gggccagtga
    22381 gacgttggca taaacatatg tgttgtcgtc gtgattaaca cacgcctgcc gctagcctgg
    22441 agcagttctg tcagccaaaa aagcaagctg gttttccccc ctgcaccaac aacagaaatc
    22501 acagtgggac gtttctgcgc gcctaaatcc atgactaatg ccgatgggtc aattatactt
    22561 ttcacgagtc tttatgacct ctctgggata aattatcccc aacttaatgc atcaggaagt
    22621 aacgcaatta tcaggcgtta ttagccccta taaataatgg aaccactatg tcagccatcg
    22681 actgtataat taccgctgcc ggattatcat caagaatggg gcaatggaaa atgatgttac
    22741 cctggcaaca gggaacaatt cttgatacaa gtatcaaaaa tgcgttgcag ttttgtagcc
    22801 gaattatttt agtcaccggc tatcgtggta atgaactgca cgaacgttat gcgaaccaga
    22861 gcaatatcac tattatccac aacccaaatt atgcgcaggg tttactaaca tctgtcaagg
    22921 ccgcagtacc cgcagtgcaa acagaacatt gttttctcac ccacggtgac atgccaaccc
    22981 tcaccattga tatttttaga aaaatctggt cgttacgaaa tgatggcgca atactgccac
    23041 tccataatgg catccccggc catccgattt tagtgtcaaa accatgcctg atgcaggcaa
    23101 tccagcgacc caatgtcacc aatatgcgtc aggcactcct tatgggagat cattattccg
    23161 tcgaaataga aaatgcagaa ataattttag atattgatac tccggacgac tttattacag
    23221 cgaagaaaag gtatactgaa atttagaaaa cgtagttaaa cgattgcgtt ca
//
LOCUS       DAZKHC020000070        22574 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZKHC020000070 DAZKHC020000000
VERSION     DAZKHC020000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.070"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            158..511
                     /locus_tag="ACHOSM_RS15975"
                     /old_locus_tag="pgaptmp_003195"
                     /pseudo
     CDS             158..511
                     /locus_tag="ACHOSM_RS15975"
                     /old_locus_tag="pgaptmp_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
     gene            523..1146
                     /locus_tag="ACHOSM_RS15980"
                     /old_locus_tag="pgaptmp_003196"
     CDS             523..1146
                     /locus_tag="ACHOSM_RS15980"
                     /old_locus_tag="pgaptmp_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nmad5 family putative nucleotide modification
                     protein"
                     /protein_id="HGU4559662.1"
                     /translation="MSSIRLTTRMKEEIARNALIKSGVFTELEEVTKLKNQLALDARV
                     IAFGGKKKTEEADQLSSKLVAISEELEKMGCSFYSYDVRSTSIYLTVSGRRVGLLSYG
                     KDGNGEDILLPTPNKDKCMFSAEHEITKRFDEICALQQNLEALKKDIESNVWAALNSV
                     TTVKRLIEVWPESKELLPKEADKASTALPALRVEDLNKMIGLPSEAA"
     gene            1210..1680
                     /locus_tag="ACHOSM_RS15985"
                     /old_locus_tag="pgaptmp_003197"
     CDS             1210..1680
                     /locus_tag="ACHOSM_RS15985"
                     /old_locus_tag="pgaptmp_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559663.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQRRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            1874..2014
                     /locus_tag="ACHOSM_RS15990"
                     /old_locus_tag="pgaptmp_003198"
     CDS             1874..2014
                     /locus_tag="ACHOSM_RS15990"
                     /old_locus_tag="pgaptmp_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HGU4559664.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDVLNQPGVPK
                     NG"
     gene            2007..2120
                     /gene="kil"
                     /locus_tag="ACHOSM_RS15995"
                     /old_locus_tag="pgaptmp_003199"
     CDS             2007..2120
                     /gene="kil"
                     /locus_tag="ACHOSM_RS15995"
                     /old_locus_tag="pgaptmp_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HGU4559665.1"
                     /translation="MDKTLMAIQTKFTIATFIGDEKMFREAVEAYRKWRSK"
     gene            2117..2305
                     /locus_tag="ACHOSM_RS16000"
                     /old_locus_tag="pgaptmp_003200"
     CDS             2117..2305
                     /locus_tag="ACHOSM_RS16000"
                     /old_locus_tag="pgaptmp_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559666.1"
                     /translation="MIPVELAKTPELSRLKREYHIAEARYWRKAGDKSKKQLCLWLAQ
                     RERMNEREFLSSPSELPF"
     gene            2314..2994
                     /locus_tag="ACHOSM_RS16005"
                     /old_locus_tag="pgaptmp_003201"
     CDS             2314..2994
                     /locus_tag="ACHOSM_RS16005"
                     /old_locus_tag="pgaptmp_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4559667.1"
                     /translation="MGTAILILGESGTGKSTSMRNINPEEAILIKPIGKPLPFKSKDW
                     LAWDARAKKGTVVTTDKWGVIVAVIKRAHEYGKRIVIVDDFQYVMSNEFMRRSEEKSF
                     DKFTEIGRHAWEVIKAAQDAPDDLRVYFLAHTEETPMGRVKMKTIGKMLDEKITVEGM
                     FTIVLRTLTRDDQFFFTTKNNGADTVKSPMGMFDSNEIDNDLSFVDATVCDYYGINNV
                     HQIKENAA"
     gene            2991..3578
                     /locus_tag="ACHOSM_RS16010"
                     /old_locus_tag="pgaptmp_003202"
     CDS             2991..3578
                     /locus_tag="ACHOSM_RS16010"
                     /old_locus_tag="pgaptmp_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HGU4559668.1"
                     /translation="MSNVIFTYNEEAALTAGQGGFINETGAYIITITEAELKQSEKGA
                     KFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGANVVHAMMGCAGIGQLTQHMVSASK
                     FVAPEFHGKKIGLVLQKVLTTNKKTGADSYQMEIRIPFIAQTGQTLKEKAEGKQPETI
                     ANMVASLKDKDNRSKNVSHNHADDYGYSQNDYPPF"
     gene            3602..3898
                     /locus_tag="ACHOSM_RS16015"
                     /old_locus_tag="pgaptmp_003203"
     CDS             3602..3898
                     /locus_tag="ACHOSM_RS16015"
                     /old_locus_tag="pgaptmp_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HGU4559669.1"
                     /translation="MPAPLYGADDPRNCSGSSKAEVLGNIKNNFDAFLALTPETKAER
                     MYRRDIQLALKQEKDRTNETAMRPLRKATIDKFPEYIDPRLRNYRSRYGAISNN"
     gene            3909..4073
                     /locus_tag="ACHOSM_RS16020"
                     /old_locus_tag="pgaptmp_003204"
     CDS             3909..4073
                     /locus_tag="ACHOSM_RS16020"
                     /old_locus_tag="pgaptmp_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HGU4559670.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRKSFCSVNDNKY
                     LNAMWRSGKK"
     gene            4070..4684
                     /locus_tag="ACHOSM_RS16025"
                     /old_locus_tag="pgaptmp_003205"
     CDS             4070..4684
                     /locus_tag="ACHOSM_RS16025"
                     /old_locus_tag="pgaptmp_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000827661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559671.1"
                     /translation="MKQMTLIEMDGFLKGKCIPCDLKVNETNAEYLVRKFAEAEAKIS
                     ALSEDQQKAIESIKQADSAVKLAHEKFSALAAENAGLKHAIAVTLDHVSVTDAGQAGV
                     AAMIINDALHHSETPATDAFLAEIRAEARNEGINYTASRLAAAFNHGFINKSLREVFD
                     VTRMILSAKEELANEPYPIDGLSGEYAEKSLEEWAEQIRKGADK"
     gene            4755..4913
                     /locus_tag="ACHOSM_RS16030"
                     /old_locus_tag="pgaptmp_003206"
                     /pseudo
     CDS             4755..4913
                     /locus_tag="ACHOSM_RS16030"
                     /old_locus_tag="pgaptmp_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            5227..5415
                     /locus_tag="ACHOSM_RS16035"
                     /old_locus_tag="pgaptmp_003207"
     CDS             5227..5415
                     /locus_tag="ACHOSM_RS16035"
                     /old_locus_tag="pgaptmp_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559672.1"
                     /translation="MKNRKAKILLVRRNAPGVWQWVRLSNRRMGLMKHYGMMDCGFCK
                     KPSAAQNRWKNHLRTKGE"
     gene            5417..5626
                     /locus_tag="ACHOSM_RS16040"
                     /old_locus_tag="pgaptmp_003208"
     CDS             5417..5626
                     /locus_tag="ACHOSM_RS16040"
                     /old_locus_tag="pgaptmp_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559673.1"
                     /translation="MAIAASYTMHLYCDCRQCTEGVYQVPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFAPGHKVLSVNK"
     gene            5623..6126
                     /locus_tag="ACHOSM_RS16045"
                     /old_locus_tag="pgaptmp_003209"
     CDS             5623..6126
                     /locus_tag="ACHOSM_RS16045"
                     /old_locus_tag="pgaptmp_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4559674.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AKPIAWECGENIILFNPDTVEAYAKRAEISPKPLYAAPPVPVTPDGWISCSEQMPEMG
                     ERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGNPTWDDGDGEDLYLKEVTHWMPLP
                     EPPQEVK"
     gene            6214..6456
                     /locus_tag="ACHOSM_RS16050"
                     /old_locus_tag="pgaptmp_003210"
     CDS             6214..6456
                     /locus_tag="ACHOSM_RS16050"
                     /old_locus_tag="pgaptmp_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4559675.1"
                     /translation="MFALIQRGQIYTDSAGYPIKILRCINNTVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQEAHLKKLRAMKRK"
     gene            6460..6594
                     /locus_tag="ACHOSM_RS16055"
                     /old_locus_tag="pgaptmp_003211"
     CDS             6460..6594
                     /locus_tag="ACHOSM_RS16055"
                     /old_locus_tag="pgaptmp_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559676.1"
                     /translation="MNKAFERWVHQRYGNRYDLTRGGDGFYCREVVKRMFEVWCHCRG
                     "
     gene            6613..6867
                     /gene="xisR"
                     /locus_tag="ACHOSM_RS16060"
                     /old_locus_tag="pgaptmp_003212"
     CDS             6613..6867
                     /gene="xisR"
                     /locus_tag="ACHOSM_RS16060"
                     /old_locus_tag="pgaptmp_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4559677.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            6901..8187
                     /locus_tag="ACHOSM_RS16065"
                     /old_locus_tag="pgaptmp_003213"
     CDS             6901..8187
                     /locus_tag="ACHOSM_RS16065"
                     /old_locus_tag="pgaptmp_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4559678.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVIKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKVG"
     gene            8223..8909
                     /gene="mlrA"
                     /locus_tag="ACHOSM_RS16070"
                     /old_locus_tag="pgaptmp_003214"
     CDS             8223..8909
                     /gene="mlrA"
                     /locus_tag="ACHOSM_RS16070"
                     /old_locus_tag="pgaptmp_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HGU4559679.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            8969..9076
                     /gene="yohO"
                     /locus_tag="ACHOSM_RS16075"
                     /old_locus_tag="pgaptmp_003215"
     CDS             8969..9076
                     /gene="yohO"
                     /locus_tag="ACHOSM_RS16075"
                     /old_locus_tag="pgaptmp_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HGU4559680.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(9057..9788)
                     /gene="yehW"
                     /locus_tag="ACHOSM_RS16080"
                     /old_locus_tag="pgaptmp_003216"
     CDS             complement(9057..9788)
                     /gene="yehW"
                     /locus_tag="ACHOSM_RS16080"
                     /old_locus_tag="pgaptmp_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HGU4559681.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(9793..10719)
                     /gene="yehX"
                     /locus_tag="ACHOSM_RS16085"
                     /old_locus_tag="pgaptmp_003217"
     CDS             complement(9793..10719)
                     /gene="yehX"
                     /locus_tag="ACHOSM_RS16085"
                     /old_locus_tag="pgaptmp_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HGU4559682.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVMNTQGQPCGTLHFQDLLEEA"
     gene            complement(10712..11869)
                     /gene="yehY"
                     /locus_tag="ACHOSM_RS16090"
                     /old_locus_tag="pgaptmp_003218"
     CDS             complement(10712..11869)
                     /gene="yehY"
                     /locus_tag="ACHOSM_RS16090"
                     /old_locus_tag="pgaptmp_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HGU4559683.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(11876..12793)
                     /gene="osmF"
                     /locus_tag="ACHOSM_RS16095"
                     /old_locus_tag="pgaptmp_003219"
     CDS             complement(11876..12793)
                     /gene="osmF"
                     /locus_tag="ACHOSM_RS16095"
                     /old_locus_tag="pgaptmp_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HGU4559684.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(13045..15342)
                     /gene="bglX"
                     /locus_tag="ACHOSM_RS16100"
                     /old_locus_tag="pgaptmp_003220"
     CDS             complement(13045..15342)
                     /gene="bglX"
                     /locus_tag="ACHOSM_RS16100"
                     /old_locus_tag="pgaptmp_003220"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HGU4559685.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEARKVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GAMVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            15538..17253
                     /gene="dld"
                     /locus_tag="ACHOSM_RS16105"
                     /old_locus_tag="pgaptmp_003221"
     CDS             15538..17253
                     /gene="dld"
                     /locus_tag="ACHOSM_RS16105"
                     /old_locus_tag="pgaptmp_003221"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU4559686.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(17291..18223)
                     /gene="pbpG"
                     /locus_tag="ACHOSM_RS16110"
                     /old_locus_tag="pgaptmp_003222"
     CDS             complement(17291..18223)
                     /gene="pbpG"
                     /locus_tag="ACHOSM_RS16110"
                     /old_locus_tag="pgaptmp_003222"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HGU4559687.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(18397..18984)
                     /gene="yohC"
                     /locus_tag="ACHOSM_RS16115"
                     /old_locus_tag="pgaptmp_003223"
     CDS             complement(18397..18984)
                     /gene="yohC"
                     /locus_tag="ACHOSM_RS16115"
                     /old_locus_tag="pgaptmp_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HGU4559688.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            19154..19732
                     /gene="yohD"
                     /locus_tag="ACHOSM_RS16120"
                     /old_locus_tag="pgaptmp_003224"
     CDS             19154..19732
                     /gene="yohD"
                     /locus_tag="ACHOSM_RS16120"
                     /old_locus_tag="pgaptmp_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4559689.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGSQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(19862..20623)
                     /gene="yohF"
                     /locus_tag="ACHOSM_RS16125"
                     /old_locus_tag="pgaptmp_003225"
     CDS             complement(19862..20623)
                     /gene="yohF"
                     /locus_tag="ACHOSM_RS16125"
                     /old_locus_tag="pgaptmp_003225"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4559690.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGVDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(20676..22112)
                     /gene="mdtQ"
                     /locus_tag="ACHOSM_RS16130"
                     /old_locus_tag="pgaptmp_003226"
     CDS             complement(20676..22112)
                     /gene="mdtQ"
                     /locus_tag="ACHOSM_RS16130"
                     /old_locus_tag="pgaptmp_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HGU4559691.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQFNDVAGNMKIIEARLSALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            22336..22419
                     /gene="yohP"
                     /locus_tag="ACHOSM_RS16135"
                     /old_locus_tag="pgaptmp_003227"
     CDS             22336..22419
                     /gene="yohP"
                     /locus_tag="ACHOSM_RS16135"
                     /old_locus_tag="pgaptmp_003227"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HGU4559692.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         6005 a         5716 c         5624 g         5229 t
ORIGIN      
        1 aaggctttgg gattggatga atgagcaggc tgatgctcga ccaatgtata aacagcgctc
       61 atggcaagca gtaaccaatc tgcgcctcaa gacagcgtca ctggtagtgc gggcgctcta
      121 accagtaagc cggggttcag cgccggccat ccaatcacca aagctaactg acaggagaat
      181 ccagatggat gcacaaacac gccgccgcga acgtcgcgca gagaaacagg ctcaatggaa
      241 agcagcaaat cccctgttag ttggggtaag cgcaaaacca gttaaccgcc ctattctctc
      301 gctgaatcgc aaaccgaaat cacgagtaga aagcgcactg aatccgatag accttacggt
      361 gctggctgaa taccacgaac agattgaaag caacctgcag cgtattgagc gcaagaatca
      421 gcgcacatgg tacagcaagc cacgcagtga aatgggtgtg acttgtgttg gtcgccagaa
      481 aatgaaatta ggcagcaaac cacttatttg agaggaatca atatgtcatc aatccgctta
      541 actacgagaa tgaaagagga aatcgctcgt aacgctttaa ttaagtctgg ggttttcact
      601 gaacttgaag aagtaacaaa gttaaagaac cagcttgcac ttgacgccag agttattgcg
      661 tttggcggta aaaagaaaac tgaggaagcg gatcagttat catccaagtt ggtagctata
      721 agtgaagaac ttgaaaagat gggatgttca ttttactcat acgatgttcg ttctacttca
      781 atttatctga ctgtatctgg cagaagggtt ggattgcttt catatgggaa agacggcaac
      841 ggcgaagata tattgctccc tactccgaac aaagataaat gcatgtttag cgcagaacac
      901 gaaataacaa aaaggtttga tgaaatctgc gcattgcaac aaaaccttga agccttgaaa
      961 aaggatatcg aatcaaatgt atgggctgct ttgaactcag taacaacagt taagcgactt
     1021 attgaagttt ggcctgaaag caaagaattg ctaccaaaag aagcagataa agcaagtaca
     1081 gcacttcctg ctttacgggt agaagatttg aataagatga ttggacttcc ttccgaggcc
     1141 gcatagtcgg cctttatttt tggcataaac aagagaataa acactgtact gaattatttg
     1201 aggtgagata tgacaaaatc atggagcgta ccttttcctg aatcagaaac tgaacatgat
     1261 ggaatgcctg ttttctggag attccaggca acagttgaag aagatgggat aaaaatattc
     1321 gcacttcaat atatagcttt tcatcagaca gagcattatg catggttggt tcctgcgcat
     1381 tggattgtta attttaaacc agcaccaaat cagtggttac aggaatggaa acaaaggaga
     1441 aatagatatg caattaagaa agtagcaaaa aatgcagaaa gatcttttgc attcccaacg
     1501 aagaaacttg ctattgaaag tttattgcgc cggaagaaat accatttaat gagaataaaa
     1561 caagatttgg ctgttgtatc aactcttgtt gatgggatga aaaatattga tacatcaaca
     1621 ccagatattg aatataactt tggacacaac caagaaacag aaaattgggt attttattag
     1681 tacgaataag cactgtgtat tcattccaac gagtgaatac acggagcaat gtcgctcgta
     1741 actaaacagg agccgacttg ttctgattat tggaaatctt ctttgccctc cagtgcgtga
     1801 gggccttttt atatgcatac caataacgct tcactcgagg cgtttttcgt tatgtataaa
     1861 taaggagcac accatgcaat atgccattgc agggtggcct gttgctggct gcccttccga
     1921 atctttactt gaacgaatca cccgtaaatt acgtgacgga tggaagcgac tcatcgacgt
     1981 acttaatcag ccaggagttc ccaaaaatgg ataaaacact tatggctatc cagactaaat
     2041 tcactatcgc cacttttatt ggcgatgaaa agatgtttcg tgaggccgtc gaagcctaca
     2101 gaaaatggag gtcaaaatga tcccggtaga actggcgaaa actccagagt taagtcgatt
     2161 aaaaagagag tatcacattg ctgaggctcg ttactggcgt aaagcgggag ataaatcaaa
     2221 gaaacaactt tgcttatggc tggcacaaag agagcgcatg aatgagcgcg aatttctttc
     2281 ctccccatcc gaattaccat tctgaggtga attatgggaa ctgcgatatt aatactcggt
     2341 gagtctggca ccggaaaatc aaccagcatg agaaatatca atccagagga agcaatactt
     2401 ataaaaccaa taggcaagcc gctaccattt aaatcaaaag actggcttgc atgggatgcc
     2461 agagcaaaaa aaggaaccgt agttaccact gacaaatggg gcgtaatagt tgccgtaatt
     2521 aagcgtgctc acgaatacgg gaaaagaatc gttattgttg atgacttcca gtatgtgatg
     2581 agcaatgagt ttatgcgccg ctcagaagaa aaatcgtttg ataaatttac tgagataggc
     2641 cgccacgcat gggaggtcat taaggctgca caggatgcgc ctgatgacct gagagtctat
     2701 tttcttgcgc acaccgaaga aacccctatg gggcgcgtga aaatgaagac tatcggcaaa
     2761 atgctggacg agaaaatcac tgtcgaaggc atgtttacta tagttcttcg cactcttacc
     2821 cgcgatgacc agttcttttt caccacgaaa aacaacggtg cagacactgt taaatcccca
     2881 atgggaatgt ttgattccaa tgagattgat aacgatctct ctttcgtcga tgccactgtt
     2941 tgtgattact acggcatcaa taatgttcat caaattaagg aaaacgccgc atgagcaacg
     3001 taatttttac ttataacgaa gaagcagcac tgaccgcagg gcaaggtggt tttattaacg
     3061 aaactggcgc ttacataatt accattactg aagcagaact caagcaatca gaaaaaggag
     3121 ccaaatttat tgagttttct ggagaatccg acgacggacg taaaatccag tatcttagcg
     3181 tctgtgttca gaaaaatgac ggcacggaaa acaaatttgg cgcaaatgtc gttcacgcca
     3241 tgatggggtg tgccgggatt ggacaattaa cgcaacatat ggtttccgcc agtaaatttg
     3301 ttgcacctga atttcatgga aagaaaatcg ggttagtgct ccagaaagta ttaaccacaa
     3361 acaaaaagac tggcgcagac agctaccaga tggaaatacg catcccgttt attgcacaaa
     3421 caggtcaaac ccttaaagaa aaggcagaag gcaagcaacc agaaactatc gccaacatgg
     3481 ttgccagcct caaagataaa gacaatcgct ctaaaaacgt aagccataat catgcagatg
     3541 attatggtta cagccagaac gattaccctc ctttctgatt actgaaaata aggctcccat
     3601 tatgccagcg cctctgtatg gtgcggatga cccgcgcaac tgctccggta gctccaaggc
     3661 ggaggtgctg ggaaatatca aaaacaattt cgacgcgttt cttgctctga caccagaaac
     3721 aaaagcagaa cggatgtacc gacgcgatat acaactcgcg ctaaaacagg agaaggaccg
     3781 aacaaacgaa acagcaatga gaccgttgcg aaaagcgaca atagacaaat tccctgaata
     3841 tatcgacccg cgcctgcgta attaccgctc acgctatggc gctatcagta ataactgagg
     3901 aatttaccat gagaggactt gcatacaatc ccggcattct tccggcagaa atgattattc
     3961 gccaacgcgt aaagccaatg ccatcgagag aggaattgct taagagaaag agtttctgct
     4021 ctgttaatga taacaaatat ctgaatgcga tgtggcggag tgggaagaaa tgaaacaaat
     4081 gacactaatt gagatggatg gatttctgaa aggtaaatgc atcccatgtg atttaaaggt
     4141 taacgaaaca aacgctgaat atcttgtgcg taaatttgct gaagcggagg ccaagatttc
     4201 ggctctgtcc gaagaccagc agaaagcgat tgagtcaatt aagcaggctg attcggctgt
     4261 taagttggca cacgagaagt tttcagcgct ggcggcggag aacgccgggc tgaaacacgc
     4321 aatagctgta actcttgatc atgtgtcggt cacggatgca gggcaggccg gagttgctgc
     4381 aatgattatc aacgatgccc tacaccacag cgaaactcca gctaccgatg cttttctggc
     4441 tgaaattcgt gcggaagcac gcaacgaggg tattaactat accgccagcc gtcttgctgc
     4501 tgcgttcaat cacggattta tcaataagtc tttgcgtgaa gttttcgacg ttacgcgcat
     4561 gattctgtca gcgaaagaag agttggctaa tgaaccgtac ccgattgatg gcctgtccgg
     4621 tgaatatgcg gagaaatccc ttgaagaatg ggcggaacag attcgcaaag gagctgacaa
     4681 gtgaagaaga tgatttttgt cgcggcattg ttggtgaata tccaacaagt gcatgcttca
     4741 gcagctattg tagcctctac cgccgcgacc aggagaacga ggatattgcg ctaacggtag
     4801 ggaagctgcg cgttgagctt gaggagacaa aatcaaaact caacgagcag cgcgagtatt
     4861 acgagggagt tatctctgat gggtgcaagc gtattgctga actggaagcg cgggaagttc
     4921 aattaccgac tctctacgac cttcgatatg gacacccgat aaatgcagat gagcgacaag
     4981 tcatgatacc taaagaaaat ggcagttggc tttacctgat tgacctagaa cacgcattac
     5041 gcgccgctgg cattcgcatc aaaggaggtg agtaatgcgt gtggcatgtc tcggcttgtt
     5101 accgtatccg actcgttttt gggcttctgc gctaattgca aagaacatca tcccgacacc
     5161 aaagcgccgc cataccggta ttgcagcggc acgacgagca gcaaagagac gcaggagagc
     5221 aaaacgatga aaaaccgtaa agcaaagatt ctgttagttc gtagaaacgc tcctggcgtc
     5281 tggcagtggg tgagactcag caaccgacgg atggggttaa tgaaacatta cgggatgatg
     5341 gattgtggtt tttgcaaaaa gcccagcgcg gcgcaaaacc gctggaaaaa ccacttgcgt
     5401 actaaaggag agtgatatgg ctattgccgc aagttacacc atgcatctct attgtgactg
     5461 ccgccagtgt acggaaggtg tatatcaagt gccagacttc ggagagtata tcggtacgtc
     5521 atgggctggt tgtgcaaaag aggcgcgtaa ggatggctgg cgaataagca aagacaaaac
     5581 acgtgctttt gcgcccgggc ataaagtttt gagtgttaac aaatgaccac tataaccaaa
     5641 gagcgactac tgacaatcaa gcagtggcgc gaaacatacg gacctggtag caacgttgta
     5701 ctgccagcag aagaagcgga agaactggca cgaattgctc tggcatcgct ggaagcaaaa
     5761 ccaatagcgt gggaatgcgg cgaaaacata atcctgttta atcctgacac agttgaagca
     5821 tacgcaaaac gtgcggagat atcacctaaa ccactatatg ccgcgccgcc agttccggta
     5881 actccggatg gttggataag ctgtagtgag caaatgccag aaatgggaga gcgacaatgc
     5941 tatgtgttag cggctgactt taaaaacaac tacccgccaa acatccccaa cactcaggtc
     6001 ggcgtatatg gcgactggtt taatgatggc aatcccactt gggatgacgg tgatggcgaa
     6061 gacctgtatc tcaaagaggt aacccactgg atgccgctac cggaaccgcc gcaggaggtg
     6121 aagtgatgaa ttggcctgaa gcattcaccg ctgtaggagt tgcaatagcg gtggcattta
     6181 ttctgtattc gcttttccgc tggggataaa ggaatgttcg ctctgattca acgtggtcag
     6241 atatacaccg atagcgccgg ctatccgata aaaattcttc gctgcataaa caacactgtg
     6301 ttgtacagaa gaatggatgg gcgaacacag tcggtaaaaa taaacgattt taatgaactg
     6361 tttgaacgga ttgatcacca ggaataccga caaattctgg cagaaacaga gcaggaagct
     6421 catctgaaaa aattacgagc catgaaaagg aagtaaagaa tgaataaagc atttgaacga
     6481 tgggtccacc agcgttacgg caatcgctat gacctgacgc gaggtggtga cggtttctac
     6541 tgtcgtgaag ttgtgaagcg aatgtttgaa gtgtggtgcc actgccgtgg atgaaaattt
     6601 tatgaggttg gcatgcaaac aatcatctat cagataaccc ccagcaaatg gtgtacggag
     6661 agagtcctca ttgcatcaac agggctaaag cctggcacca ttgagcgggc aagaagaaag
     6721 tcatggatgc agggaaaaga ataccgccat tacgctgtag aaggtgatcc ggggcactac
     6781 agtgaatgcc tgtacaacat cgaagaaatt atgcgatgga tcgaaaacca gaaacaacca
     6841 ggtgccaaaa atgcaagttc cggttaacct gttaatgctc ctggacgtct gggaggttta
     6901 atgagtaacg catcataccc gacaggcgtt gaaaaccatg gaggatcact ccgtatatgg
     6961 tttcactata atggcaaacg tgtcagagaa aacctcggtg ttcctgacac agccaaaaac
     7021 cggaagatcg ctggtgaact tcgcacttcc gtttgttttg caatcagaat ggggagtttc
     7081 gactacgccg cgcagttccc taattcccct aacctgaaac actttggtct gggaaaaaga
     7141 gagataaccg ttaaggcact ttcggaaaaa tggttggacc ttaagaaaat tgagatttgt
     7201 gcgaatgcac ttaaccgtta ccagtcagta attaaaaaca tgttaccaat gttaggtgaa
     7261 aaaaaactgg tttcatccat aacaaaagag gatttacttt tcgtaaggag agatttgttg
     7321 accggttacc aaaagctttc taatggaaag acttcttcca taaaagggcg ctcagtggtc
     7381 acggtaaact actatatgac aaccatagct ggaatgtttc aatttgcaac agataatggt
     7441 tatacctcag gaaacccatt taacggtctg gctcccttaa aaaagtccaa ggtaaaacca
     7501 gatcctctca cccgtgacga atttattcgt tttattgagg cctgccgtca tcaacaaaca
     7561 aaaaacctgt ggattctcgc tgtatacacg ggtattcgtc acggggagtt ggtatcgctg
     7621 gcatgggaag atatagacct taaagcaagg actataacca tccgtagaaa ttatacaaaa
     7681 cttggcgaat tcactccacc aaaaaccgat gctggcaccg gaaggacaat tcatctggtt
     7741 caaccagcta ttgatgctct taaaagccag gcggaaatga ccatgcttgg aaagcaacat
     7801 tctgtagagg taaagcagag ggaatatggg agaacagctg tgcataaatg tacttttgtt
     7861 tttagccctc aggtaataaa acagcagcag ttgtctggac ctcactacaa agttgactcc
     7921 atcagggagt catggacaag tatcttaaaa cgcgcaggtc tgagacacag aaaatcgtac
     7981 caatccaggc atacttatgc atgctggtca cttgccgctg gagctaatcc tagttttatc
     8041 gcaagccaga tgggccacac aaacgcacaa atggtattca atgtttacgg agcatggatg
     8101 aaagacaaca atcacgaaca gatagaactc cttaacaaaa gactatctga aagtgtccca
     8161 tgtatgcccc ataagaaagt ggggtaaaat aaaaacttgt aaaatcagtt agtttaccct
     8221 taatccctgt cacgttacgc gcgtggcaga ggcgttacgg gttgctgaaa ccgcaacgga
     8281 cagacggcgg tcatcgactg ttcaacgatg ccgatattga ccggatccgc gagatcaaac
     8341 gctggatcga caacggcgtg caggtcagca aagttaaaat gctgctcagt aatgaaaatg
     8401 ttgatgtgca gaacggctgg cgcgatcagc aagaaacatt actgacctac ctgcaaagcg
     8461 gcaatctgca tagcctgcga acgtggatca aagagcgcgg tcaagattac cccgcccaga
     8521 cactcaccac acatctgttt attcctctgc gccgacggct tcagtgccaa caaccgactc
     8581 tccaggcgct gctggcgatc ctcgacggcg tactgatcaa ctacatcgcc atttgtctgg
     8641 cttcggcacg taaaaaacag ggtaaagatg cgctggtggt tggctggaat attcaggata
     8701 ccacccgtct gtggctggag ggctggattg ccagtcaaca aggatggcgc attgatgtcc
     8761 tcgcccactc gctcaatcaa ctacgccctg aactattcga aggccgtaca ttgctggtgt
     8821 ggtgcggtga aaatcgaacc tccgcccaac agcagcaact caccagttgg caagaacaag
     8881 gccatgatat tttcccactc ggcatttaat gattcgttaa caaatgcgct ttactgtaca
     8941 atcctttcgt taacataagg agtgcattat gcgcatagct aaaattgggg tcatcgccct
     9001 gttcctgttt atggcgttag gcggaattgg tggcgtcatg ctcgcaggtt atacctttat
     9061 tttgcgtgct ggctaagcgc ctgcaccagc ctttcaaaca ggcggtctgc gatgatcgcc
     9121 gccagtgcca ccagtaacgc cccctggatc acatacgcgg tattaaatcc gctaagcccg
     9181 atgatgatgg gcgtacccag cgtgctggcc cctaccgttg aggcgatcgt cgccgtacca
     9241 atgttgataa tcaccgaagt tcgcacgccc gccagaatca ccggagccgc cagcggtagc
     9301 tcgaccttac gcagtcgctg accacgactc attcccatac ctttcgcaac ttctgtcacg
     9361 ctggcatcaa tcgctcccag cccggcaagt gtcgcctgca ggacgggcag cacaccgtaa
     9421 aggatcaagg cgataatcgc tggttgcaga ccaaagccga tcaccggaac ggcgatcgcc
     9481 agcactgcga cgggcggaaa agtctgtcca acggcggcaa tagtttccac cagtgggcga
     9541 aattccgcgc cccacgggcg agtgacagca attccggcac cagtaccaat gatcaccgca
     9601 aacaaactcg aaattcccac cagccagaaa tgagccagtg ccagagctgc aaaactttct
     9661 tgctgataaa cgggtcgtgg cagttgtggg aacaaggcag caaacagcgg ctggctgtaa
     9721 ggcagccaga aaatcagtgc cacaaacaaa gcaatgagcc agaacagcgg atcgcgcaac
     9781 attttcatac gcttacgcct cctccagcag atcctgaaaa tgcagcgtgc cgcaaggctg
     9841 gccctgcgtg ttcatcaccg gcagcacctc gcatccccgc gcgacaaaca gggagagcgc
     9901 atcgcgtagc gtcatctctt ctgccagtgc ctcaccttct gcccgttctt cgcgacgcac
     9961 gtaatccgcc acactacgta acgaaagcag gcgcaccccc agttcactac gtccaaaaaa
    10021 ctggcggaca aaatcattcg ccggacgagt cagcatcgtc agcggattcc cctgctgcac
    10081 cacttcaccg tgatccatca ataccagatg ttctgccagc cttagcgcct catcaatatc
    10141 atgagtgacc agcacaatag tgcgccccag caaacggtga atgcgcgtca tctcttgttg
    10201 caacgcgccg cgcgttaccg ggtccagtgc gccaaaaggt tcatccatca gtaagacttg
    10261 cggatcggca gccagtgcgc gcgccactcc cacacgttgc tgctgaccac cggaaagctg
    10321 atgcggataa cgctcacgca aatttgactc cagccccagt agcgccatta attcgtcgat
    10381 acgatcgtca atccgcgccc gcgaccattt ttgtaattgc ggcacggtag cgatgttttg
    10441 cgccacgctc cagtggggga acaggccaat agattgaatg gcatagccca tccggcggcg
    10501 caactccagt actggcagcg agcgaatttc ttctcctgca aagcggatca ctccgctgtc
    10561 atgctccacc aggcggttaa tcattttcag ggtggtggat ttgccggagc cagatgtgcc
    10621 aatcagcacc gaaaaactcc cttcctgaaa attgagattg agatcgttaa cggctttttg
    10681 tgcgccgaac agtttgctga catggctaaa ttcaatcatt acgtttcacc ttcagcagtg
    10741 cgataagcaa atcgaacagc gcgtcggtaa gcaccgccag aacaattacc gggatcaccc
    10801 ccagcaacac taaatcaatg gcgctgctta gcagcccctg gaaaaccagc gcaccaaaac
    10861 cgcctgcgcc gattaacgcc gcaatcaccg ccatacctac agtttgcacc atcaccaccc
    10921 gcaggctgcg cagaaatacc ggtaacgcca gtggtaactg aacatgcagg aatcgctgcg
    10981 ccccgctcat gcccatcgct ctggcgctct ccagcacatc gcgcgggatc tggttcaaac
    11041 cgactaccac gccgcgcacc agcggcagca aggcatagag caccagcgca atcagtgcgg
    11101 gtgtcattcc ggttcctgct atgccgagcg tccccagcca cggaaaggcc gtaaccagcg
    11161 cggcaagcgg cgcaatcaac aggccaaaga gcgccaccga aggcacggtc tgaatgacat
    11221 tgagcagaga aaaaattgcc ccctgccgag cagtggaaaa gtagcaccag atgcccaacg
    11281 gcacaccaat cactaacgca ggcagcaccg caccaaacag caacgtcaga tgttgtgcca
    11341 gcgcgtcgtc aaacacatcc tgacggttgg cgtattcttt cattagtgag agatcgttaa
    11401 gcgtgccgga gtacagcaac cacagcggaa taatggcaat ctgcatatgc aacaaccagc
    11461 gccacagcgg atgcgtggag attcggcgga tggcatcgct acaggccagc aatgccagcg
    11521 ccgcagccag ccagaaacca ctgccgaggc tggtacgcgc cagcgcactg ccattttgcg
    11581 ccagttgtgt cgccgccttt cccgcgcccc acaccagcaa tacgaagacg aattgcgcca
    11641 gaatgagtgc acaaatgctg ccttttttac cgggaataaa acaggccgtc agccaggcgc
    11701 aaccaacgcc caccagcatc cagatcgttt gcggccacag ctgccagaga tgacgcccct
    11761 cacccgaaac taaacgatta ggcgcgtaac tgataaacgg cagcgccgct gcgattgccg
    11821 tcagcaacag cagcagcgcc agaacaggat taatacggaa ataagtcacg ggaaattact
    11881 tcgcccaccc tttttgtttc aggtagtcgg cagccacttt tttggcatcc agtccttcca
    11941 ctgcaatgct ggcattcagt tgctgcaatg tttttgcatc gaggctggcg aagactggct
    12001 gtagccactg tgccatttgc ggatactcct tcaacaccga ttcacgcacc actggtgcag
    12061 gcgcgtagat aggttgcacg ccttgcggat cgcttaaggt ttgcagcccc agcgccgcga
    12121 ccggaccgtc agtgccgtaa gccattgcgg cattaacgcc agaggtttgc tgggcagcgg
    12181 ctttaatcgt caccgccgtg tcgccaccag ccagtgacag caactgatcc tgaccgagct
    12241 taaagccata ggctttttca aacgcgggta acgcatcggc gcgttcgata aactctgccg
    12301 aggctgccag tttgaaggtg ccgccctctt tcagataacg actcaggtcg gcaagcgaag
    12361 tgagtttgtt tttctctgcc acatcctgac gcacggcgat ggtccaggtg ttattcgctg
    12421 gtgcgggcgt cagccagatt aacttgtttt gctctgcatc gagttttttg actttctcgt
    12481 aaccttgctg cgcgtttttc catgccgcat cgttttcatc tttaaagaag aaagcgccat
    12541 taccggtata ttccggatag atatccagtt caccggaagt aatcgccccg cgcaccacag
    12601 gagtcgttcc gagttgcact ttattcaccg tcggtacgcc gtggctttcc agcacctgca
    12661 agatgatgtt gccgagtagc gcgccttcgg tatcgatttt tgaaccgact ttaacggggg
    12721 aagccgcctg tagcggcagg ctcacggctg ccaacataac cagtgaacct gcccagacct
    12781 ttgagagtgg catgatgctt tcctcattct tttactgttg ttttcagcga attaagagaa
    12841 aagcatagtt gataatgaca gagttagcct gagcggaagg attcagttgc agaatcagat
    12901 aaatacttaa agggcatatt ttgcccggcg tttcttgccg gatgcggcgt aaacgcctta
    12961 tccggcctac aaagggcgaa aattcagcaa gttaagatat gaacatcagg caaagggcgc
    13021 aaacgtcgcg cccttactaa agcattacag caactcaaac tcgcctttct taacgcgtgc
    13081 ggaatcagtg ccgataaata cattgaactt gccaggctcg gcgtcatatt tcatctgttg
    13141 attccagaac ttcagcgcct caatatcaat cgggaagctg acagtctgag tttcgcccgg
    13201 tttcagggtg attttctcaa agcctttcag ctgcttcact ggacggctca tggaagccgt
    13261 cacatcctgc aagtacatct gtactaccat ggccccttcg cgtttaccgg tgttcgtcac
    13321 ctgcacgctg gcggtcactt tgccgtcacg cttcatggtc ggcgcagaaa gtttcacatc
    13381 agagacggtg aaagtggtat agctcagacc atagccgaac ggataaagcg ccccgttagc
    13441 ttcatcaaaa taacgcgaag tgtatttgtt cggcttgtcg gcattatacg gacgaccggt
    13501 attcagatgg ctgtagtaca ccgggatctg cccgacagaa cgcgggaagg acattggcag
    13561 cttgccggac gggttgtaat cgccaaacaa tacatcggca attgcattac cgccttcagt
    13621 ccccgcaaac caggtttcca gaatcgcatc agcctgctga tcttctttca ccagcgccag
    13681 cggacgcccg ttcatcagca ccagcaccag cggtttaccg gtggctttca gcgccgcaat
    13741 caagtcacgt tggctttgcg gaatagtgat atcggtacgg ctggaggcct cgtgcgccat
    13801 cccctgtgct tcaccgacca cagccaccac cacatcagat tgtttcgccg tctgcaccgc
    13861 ttcatcaatc atctcttgcg gcgagcgcgg atcgactttg accgcttctt catactgatt
    13921 caggaaatcg ataatgcctt tgtcactggt aacgttcgcc cctttggcat acagcacttt
    13981 accgttttca ccgacggcat ttttaatccc ggtcagtacg gtcacggatt gatcggcaac
    14041 accggctgcg gaccagctgc ccatcacgtc acgtttactg tccgccagtg gcccaaccac
    14101 cgcaatggtg gccgattttt tcagcggtaa cgtttcgaga cggtttttca gcaacaccag
    14161 gctttcacgc gccactttac gcgcttcttt gcggtgcagg cggctttcgg cattagtgtc
    14221 aaccgggtca gactctttcg gccccaaatg gctgtatggg tcgttaaaca accccatatc
    14281 atatttaacg ttcagtacat ggcgggcagc atcgtccagc tctgccatcg tcactttgcc
    14341 ggatttgatc aacccaggca gatacttcga gtagtactcg tcgctcatgc tcatgttgat
    14401 tccggatttc agcgccacgc gcaccgcatc ttccgggtct gccgccgtgc catgtttaat
    14461 cagctctttg attgcaccgt gatcggaaac ggtgatgcct ttaaagcccc actggtcgcg
    14521 cagaacatct ttcagcagcc aggagtcgga ggtggctggc gtgccgttca gcgagttcag
    14581 cgccaccatc accgcgccgc tgcctgcgtc cagccccgct ttgtacggcg gcatatagtc
    14641 gttaaacagg cgctgcggac tcatatcgac ggtgttgtac tctttaccgc cttctaccgc
    14701 gccgtatgcg gcaaagtgtt tgacgctggt catcaccgag tagcgatctg ccgggctttt
    14761 accctgcatc gcttccacca tggttttacc cattgttgag gtgagatacg tatcttcgcc
    14821 aaaaccttcg gaagcacgtc cccagcgcgg atcgcgcgag acatcgacca tcggtgccca
    14881 ggtcatattc aggccatcat ctgccgcttc ataagcagag acacgtccga ccgttttcac
    14941 tgcatcgagg ttaaaagacg aggccagacc gaggctaatc gggaacaccg tgcgctgacc
    15001 gtgcagcacg tcgtaagcaa agaaaagagg aattttcagg cggcttaatt ccatcacctg
    15061 atcctgcatg gcgcggatat cctgacgggt tacggtgttg aaaatcgccc caacctgacc
    15121 gtctttgatc atctcgcgga tcgcctcttt cgggttatcc gggccgacgc tgattaagcg
    15181 cagctgacca attttctcat caactgtcat tttcttaagc agttcggtga cgaacgcatc
    15241 ccgcgcttcg ggcgttaatg gatggttgcc gaataaatca tccgccagtg ctggctgcag
    15301 ggccagactc accgcgattc ctactgaaca tagccatttc atatggattt ttcttcctct
    15361 gttgcccgac gaggcagcga aaatggtgca aaaaccgtag tttgccataa gcatgatgga
    15421 gagagaaaaa gaatgctcag tttattgtct gaattttcaa aatattcact cgctgaattg
    15481 tcatacaagg cgctattcta gtttgtgata ttttttcgcc accacaagga gtggaaaatg
    15541 tcttccatga caacaactga taataaagcc tttttgaatg aacttgcccg tctggtcggt
    15601 cattcacacc tgctcaccga tcccgcaaaa acggcccgct atcgcaaggg cttccgttct
    15661 ggtcagggcg acgcgctggc tgtcgttttc cctggctcac tactggaatt gtggcgggtg
    15721 ctgaaagcct gcgtcaccgc cgacaaaatt attctgatgc aggctgccaa tacaggcctg
    15781 accgaaggat cgacgccaaa cggtaacgat tatgatcgcg atgtcgttat catcagcacc
    15841 ctgcgtctcg acaagctgca cgttcttggc aagggcgaac aggtgctggc ctatccgggc
    15901 accacgctct attcgctgga aaaagccctc aaaccgctgg gacgcgaacc gcactcagtg
    15961 attggatcat cgtgtatagg cgcatcggtc atcggcggta tttgtaacaa ctccggcggc
    16021 tcgctggtgc aacgtggccc ggcgtatacc gaaatgtcgt tattcgcgcg tataaatgaa
    16081 gacggcaaac tgacgctagt gaaccatctg gggattgatc tgggcgaaac gccggagcag
    16141 atccttagca agctggatga tgatcgcatc aaagatgacg atgtgcgtca cgatggtcgt
    16201 cacgcccacg attatgacta tgtccaccgc gttcgtgata ttgaagccga cacgcccgca
    16261 cgttataacg ccgatcctga tcggttattt gaatcttctg gttgcgccgg gaagctggcg
    16321 gtctttgcag tacgtctaga taccttcgaa gcggaaaaaa atcagcaggt gttttatatc
    16381 ggcaccaacc agccggaagt gctgaccgaa atccgccgtc atattctggc taacttcgaa
    16441 aatctgccgg ttgccgggga atatatgcac cgggatatct acgatattgc ggaaaaatac
    16501 ggcaaagaca ccttcctgat gattgataag ttaggcaccg acaagatgcc gttcttcttt
    16561 aatctcaagg gacgcaccga tgcgatgctg gagaaagtga aattcttccg tccgcatttt
    16621 actgaccgtg cgatgcaaaa attcggtcac ctgttcccca gccatttacc gccgcgcatg
    16681 aaaaactggc gcgataaata cgagcatcat ctgctgttaa aaatggcggg cgatggcgtg
    16741 ggcgaagcca aatcgtggtt ggtggattat ttcaaacagg ccgaaggcga tttctttgtc
    16801 tgtacgccgg aggaaggcag caaagcgttt ttacaccgtt tcgccgctgc gggcgcagca
    16861 attcgttatc aggcggtgca ttccgatgaa gtcgaagaca ttctggcgtt ggatatcgct
    16921 ctgcggcgta acgacaccga gtggtatgag catttaccgc cggagatcga cagccagctg
    16981 gtgcacaagc tctattacgg ccattttatg tgctatgtct tccatcagga ttacatagtg
    17041 aaaaaaggcg tggatgtgca tgcgttaaaa gaacagatgc tggaactgct acagcagcgc
    17101 ggcgcgcagt accctgccga gcataacgtc ggtcatttgt ataaagcacc ggagacgttg
    17161 cagaagttct atcgcgagaa cgatccgacc aacagcatga atccggggat cggtaaaacc
    17221 agtaaacgga aaaactggca ggaagtggag taaaaattac ggatggcaga gtatcgccat
    17281 ccgaattcac ttaatcgttc tgtgccgtct gccccgccgc cgccatttgg gcggcttttt
    17341 gttttttata gctcaacgct gctgccggca caggcatcac tttaccggtt tcaatccagg
    17401 tacgcaggcg gctggcatcg gcaaaatggg tatatttgcc aaacgcgtcc atcactacca
    17461 gcgccaccgg tttattattg ataaccgtac gcatcaccag acaatggccc gccgcattgg
    17521 taaagccggt tttggttaac tgaatattcc agttatcgcg atacaccaga tgattagtat
    17581 tgcggaacgg cagcgtatac gtcgggttag agaaggttgc catatcttcc cgggtagtac
    17641 ttaactgccc gatcaacgga tattgtttgc tggcaatgag cagtttggtt aagtcacggg
    17701 cagttgaaac gttatgcacc gacaatccgg taggttcaac aaagcgcgtg ttgttcattc
    17761 cgagcgattt cgctttcgca ttcattgcct taataaaggc tttgtaacca ccgggataat
    17821 ggtgcgcaag gcttgccgcc gcgcggtttt ctgaagacat cagcgccagc aacagcatat
    17881 ctttacggct gatttcgcta ttcagtcgta cgcgcgaata gacccctttc atctccggcg
    17941 tctggctgat atccactttt agtttttcat ccagcggcag tcgtgcatcc agcacaacca
    18001 tcgcggtcat taatttgctg atagacgcaa tcggacgcac cagatccggg tggttcgaat
    18061 agatcacttt gttggtattc agatcaacaa tcatcgcgct accggaggca atttccggtt
    18121 gtgaagcggt ggtagcggct gccgttttcg caacggcctg cggtgccaaa ggcacagcca
    18181 gcatcagggc caggctaaat aaagaaactc gaaatttcgg catgatgagc attcagatag
    18241 tggttcacgc gcacgggttg cgcaccgccg gagtaaggat ttactgaggc tagcgacgcc
    18301 atcataacga gcaaaaagtg cgatcgtcaa aggagaatcg tgaggaaatg ctgcattgct
    18361 gacatttacg ccagcaatgc aatgtcaaag aactttctag aacaggcgat acccgtagcc
    18421 ccatagtata acggttaggg cgagcagcac ttccagtacc agcacgccaa tcgccagcgt
    18481 cgaactggag aagctaaggc cttcctcttt gttgatattc aggaagctcg gaataccaag
    18541 gtaaagcagg tagccggtgt aaaacagcgc caccgtgccg accagcgcac acaaccagac
    18601 cagcggataa agcgccacca gaccgcttaa aaacagcgga gttgcaacgt agcccgcgaa
    18661 gaccatgcag tgcgcaagtg acggacgctg cgggtaattg cgcgccatcc accagatgac
    18721 ccgccccatc accgcgaccc cagcgagcat aacgccataa aacaggacag ccaacgccag
    18781 tccggtaaac caggataact tcaggatagt gccatcgcca aaattccagc caatctgtgt
    18841 agtgccaatg aaggcgcaaa tcaccggaat cgccgccatc agcaaaacgt ggtgggtgta
    18901 atggtgagaa atcgtttcgt tttcgcgatt aatcacctgc atttcacgat cgggatggga
    18961 aaacagtccc catacatggc tcataccgcc cccttgttgt gagttcatga acctgacagt
    19021 tcaagtataa gtcagcttgt gattattttt tgttcgctca tgtaatttgc accgtctgat
    19081 gtacgtcctt tttcagcgtg aatgatttcc agggtgtatg cttacaggca accaaaggga
    19141 gacagactgg cctatggatc tcaatacact catctcacaa tatggttatg ccgcgctggt
    19201 gatcggtagc ctggcggaag gtgaaaccgt gactttgctg ggaggcgttg cggcacatca
    19261 ggggctatta aagttcccgc tggtggtact ttctgtggcg cttggcggca tgattggcga
    19321 ccaggtgctt tatctgtgcg ggcggcggtt tggcggcaag ctgttacgcc gtttctcgaa
    19381 acatcaggat aaaattgagc gggcgcagaa acttatccaa cgccatccgt atctgtttgt
    19441 cattggtacg cgctttatgt atggctttcg ggtgattggc ccgacgctga ttggtgccag
    19501 ccagctgccg ccgaaaatct ttctgccgct gaatattctc ggcgcatttg cctgggcgtt
    19561 gatttttacc actattggtt acgctggtag tcaggtgatt gcgccgtggt tgcacaatct
    19621 cgaccagcat ttgaagcact gggtctggtt gattctggtc gtggttctgg tggtgggagt
    19681 gcgctggtgg ctgaaacggc gcgggaagaa aaagccggat catcaggcgt aaaaccattg
    19741 ccctgggtaa ggcgttcgcg ccgcatccga catccggtgc accatgcctg atgcgacgct
    19801 tgtgcgtctt atcatgccta ccaaaatttt acccattgaa ataaccacgc acctcactgc
    19861 gctattctgg gttgaactgc ggattcgcca acataaagcc gccatccact atcaacgact
    19921 gcccggtggt gtaatttgcg ccctctgagc acagccacgc taccaggctg gcaatctcat
    19981 gcgttgcgcc aaaacgccgc agaggaatcg aaggctccgc gtcgggcttc acgtcgctgt
    20041 catccatgcc attcattggc gtggcgatcg ccccaggcgc gactgcgttc accaaaatct
    20101 tatgcctgac cagctccagc gccatcgctt tggttaaccc accgagcgca tgtttagcgg
    20161 ctgtgtaggc gctggcatcc ggcagcggcg tatgttcatg taccgacgta atgttgatga
    20221 tgcgaccgcc ctgcccttgt ttcaccatct ggcgggccgc aatttgtgag cataaaaatg
    20281 caccatcgac atcaacggta aaaatcttgc gccactcatc gaaagccata tcaagaaacg
    20341 gcgctttggt cattgcgccc gcattattca ccagcacatc aatacgcccc agccgttgaa
    20401 tgagtttctc cagcgcctgc gcaccttctg gcagtttacc gagatccagc tgcacgatct
    20461 ccgcacgtac gccgtggcta actacctcac gcgcggtatc ttttgcccct tcttcatctg
    20521 agtgccaggt aataccaata tcaaccccct gctgcgccag taataacgcg cactctttgc
    20581 cgatccccga atcggaggcg gtaataatcg caacctgtgc catcgagttc tccacttaac
    20641 gctgaataaa cgttaagtat agaaggcgca tatcatcagc gtttgtaacc cccgcccaac
    20701 gcaccagtga gttgaatgga agcatccagc cactgccctt gcaataacag gccattggcc
    20761 cgctcacgca gcgcggggat tctggcttcg ctgacgcggg aaccagcaat gatgcccgcg
    20821 ttaaagcgcg cctgcgcaag acctaccaca cgcaaggcat cgcgctcaat ttgcgcctga
    20881 tgctggtttt tctccgcaag tgtctgaacc tgactggctg cccgcgccac gtcattcacc
    20941 gcttcaacca ccgctttgtt gtagctggcg atagacaagt tgctttcggc ttttgcgata
    21001 tcgagattgg cgttaagacg accactatcg aaaataggta gcgtcaggcc tgccgtaacg
    21061 cccatttgct gcgcggaatg acggaacaga tcgcttaagt gcaacgcatc ctgttgcagg
    21121 aaggccatca ggttgatgtc aggataaaat gccgcttttg ccgcatcaat ggtgcttagc
    21181 gatgactcaa cgtaccagtg cgccgcctgc aaatctgccc gccgggccag taaggagtac
    21241 cccagttcat caggaagctg gcttgccact ttcggcaacg cgaccggttt aagcttcaat
    21301 gactttgtct ggtgatttgt aagtgcgctt aaccgtgcct caataatttt catattcccc
    21361 gcgacatcgt tgaactgctg ccgggttttg ctggcattaa tatcggtttc cacaccgtca
    21421 actgaagaag taatcccgtt ctgatatagc tggcgatcgg tcgcgataat ggtgttctgc
    21481 tctttttcta tttgctgcaa gaccgtgttt aacgccgcct gggtttgcca ctcccagtac
    21541 aggcgggcta cgctgccagc cagcaattgg cgggtttgct cgcgttccgc cgcccgtgct
    21601 ttaaccgtac ccaggcgggc agtaacctcc gcccgattct ttccccagat atcgagatgc
    21661 cagcccgccg ttaagccaaa agttccgttg gtgtaccacg gaccggtcgt acctgcggcc
    21721 ggatcgttca gagcaaacgg ccccattagc ccttctgccg acattttttg ccgctccata
    21781 tccgccgaaa agtcgatctg cggaccatcc tgagtggcaa ctgccttcgc ctgggcttca
    21841 gctagctgaa tgcgctgttc agccacctgc atatccggtg cgttctgtag tgcattgtta
    21901 attaatgaag tgagctgatt atcgtgatac tccagccacc attggctgtc tggccaacca
    21961 tttttcagcg ccgtgggtaa tgcggtgtca acttgtgcag cgggcgtttg ctggcttaac
    22021 gcctggcggg tttcatgcat aggcgcacac ccggccagca tcagtaacag cggaaaacag
    22081 gcgatggctg gataaaagga atcacgattc atgggggaat aatcaggtaa gaaacggtgc
    22141 gcggagatta ccgtgtgttg cgatatattt tttagtttcg cgtggcaata catcagtagc
    22201 aataaaacga catacccaga aaaatataga ctaagtaaat gatatcttcc gatttatctt
    22261 aatcgtttat ggataacggc aaagggcttc gttttttcct atacttattc agcactcaca
    22321 aataaaggaa cgccaatgaa aattatactc tgggctgtat tgattatttt cctgattggg
    22381 ctactggtgg tgactggcgt atttaagatg atattttaaa aatcagatat ggcatataag
    22441 ttgtcaggtc aggaactaga tattttgtgt acttgatttg caaacagctc cggtgttggt
    22501 ggcgtcaaaa tcgctgaagc gttccccgaa ggccggggca gcccacatgg atgtgggctg
    22561 agggcgcgtc ttac
//
LOCUS       DAZKHC020000071        21835 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZKHC020000071 DAZKHC020000000
VERSION     DAZKHC020000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.071"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..551)
                     /gene="lacA"
                     /locus_tag="ACHOSM_RS16140"
                     /old_locus_tag="pgaptmp_003228"
     CDS             complement(<1..551)
                     /gene="lacA"
                     /locus_tag="ACHOSM_RS16140"
                     /old_locus_tag="pgaptmp_003228"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HGU4559693.1"
                     /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPDVTIGDNSVIGA
                     SSIVTKDIPPNVVAAGVPCRVIR"
     gene            complement(617..1870)
                     /gene="lacY"
                     /locus_tag="ACHOSM_RS16145"
                     /old_locus_tag="pgaptmp_003229"
     CDS             complement(617..1870)
                     /gene="lacY"
                     /locus_tag="ACHOSM_RS16145"
                     /old_locus_tag="pgaptmp_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HGU4559694.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKNLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(1922..4996)
                     /gene="lacZ"
                     /locus_tag="ACHOSM_RS16150"
                     /old_locus_tag="pgaptmp_003230"
     CDS             complement(1922..4996)
                     /gene="lacZ"
                     /locus_tag="ACHOSM_RS16150"
                     /old_locus_tag="pgaptmp_003230"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGU4559695.1"
                     /translation="MTMITDSLVVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLHAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTHPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(5119..6201)
                     /gene="lacI"
                     /locus_tag="ACHOSM_RS16155"
                     /old_locus_tag="pgaptmp_003231"
     CDS             complement(5119..6201)
                     /gene="lacI"
                     /locus_tag="ACHOSM_RS16155"
                     /old_locus_tag="pgaptmp_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HGU4559696.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVVTSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDATSVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(6278..7111)
                     /gene="mhpR"
                     /locus_tag="ACHOSM_RS16160"
                     /old_locus_tag="pgaptmp_003232"
     CDS             complement(6278..7111)
                     /gene="mhpR"
                     /locus_tag="ACHOSM_RS16160"
                     /old_locus_tag="pgaptmp_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HGU4559697.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLQAILARARKEGYGQNYRSWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            7302..8966
                     /gene="mhpA"
                     /locus_tag="ACHOSM_RS16165"
                     /old_locus_tag="pgaptmp_003233"
     CDS             7302..8966
                     /gene="mhpA"
                     /locus_tag="ACHOSM_RS16165"
                     /old_locus_tag="pgaptmp_003233"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HGU4559698.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFARELEAFSQQNDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALMREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            8968..9912
                     /gene="mhpB"
                     /locus_tag="ACHOSM_RS16170"
                     /old_locus_tag="pgaptmp_003234"
     CDS             8968..9912
                     /gene="mhpB"
                     /locus_tag="ACHOSM_RS16170"
                     /old_locus_tag="pgaptmp_003234"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HGU4559699.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            9930..10796
                     /gene="mhpC"
                     /locus_tag="ACHOSM_RS16175"
                     /old_locus_tag="pgaptmp_003235"
     CDS             9930..10796
                     /gene="mhpC"
                     /locus_tag="ACHOSM_RS16175"
                     /old_locus_tag="pgaptmp_003235"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HGU4559700.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFRRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            10806..11615
                     /gene="mhpD"
                     /locus_tag="ACHOSM_RS16180"
                     /old_locus_tag="pgaptmp_003236"
     CDS             10806..11615
                     /gene="mhpD"
                     /locus_tag="ACHOSM_RS16180"
                     /old_locus_tag="pgaptmp_003236"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HGU4559701.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSTAPKGSLS"
     gene            11612..12562
                     /gene="mhpF"
                     /locus_tag="ACHOSM_RS16185"
                     /old_locus_tag="pgaptmp_003237"
     CDS             11612..12562
                     /gene="mhpF"
                     /locus_tag="ACHOSM_RS16185"
                     /old_locus_tag="pgaptmp_003237"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HGU4559702.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            12559..13572
                     /gene="mhpE"
                     /locus_tag="ACHOSM_RS16190"
                     /old_locus_tag="pgaptmp_003238"
     CDS             12559..13572
                     /gene="mhpE"
                     /locus_tag="ACHOSM_RS16190"
                     /old_locus_tag="pgaptmp_003238"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HGU4559703.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            13758..14969
                     /gene="mhpT"
                     /locus_tag="ACHOSM_RS16195"
                     /old_locus_tag="pgaptmp_003239"
     CDS             13758..14969
                     /gene="mhpT"
                     /locus_tag="ACHOSM_RS16195"
                     /old_locus_tag="pgaptmp_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HGU4559704.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            15071..15610
                     /gene="yaiL"
                     /locus_tag="ACHOSM_RS16200"
                     /old_locus_tag="pgaptmp_003240"
     CDS             15071..15610
                     /gene="yaiL"
                     /locus_tag="ACHOSM_RS16200"
                     /old_locus_tag="pgaptmp_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HGU4559705.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(15836..16669)
                     /gene="fghA"
                     /locus_tag="ACHOSM_RS16205"
                     /old_locus_tag="pgaptmp_003241"
     CDS             complement(15836..16669)
                     /gene="fghA"
                     /locus_tag="ACHOSM_RS16205"
                     /old_locus_tag="pgaptmp_003241"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU4559706.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMHHFPATAKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLAENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     VSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(16763..17872)
                     /gene="frmA"
                     /locus_tag="ACHOSM_RS16210"
                     /old_locus_tag="pgaptmp_003242"
                     /pseudo
     CDS             complement(16763..17872)
                     /gene="frmA"
                     /locus_tag="ACHOSM_RS16210"
                     /old_locus_tag="pgaptmp_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
     gene            complement(17907..18182)
                     /gene="frmR"
                     /locus_tag="ACHOSM_RS16215"
                     /old_locus_tag="pgaptmp_003243"
     CDS             complement(17907..18182)
                     /gene="frmR"
                     /locus_tag="ACHOSM_RS16215"
                     /old_locus_tag="pgaptmp_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HGU4559707.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(18367..19140)
                     /gene="yaiO"
                     /locus_tag="ACHOSM_RS16220"
                     /old_locus_tag="pgaptmp_003244"
     CDS             complement(18367..19140)
                     /gene="yaiO"
                     /locus_tag="ACHOSM_RS16220"
                     /old_locus_tag="pgaptmp_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HGU4559708.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            complement(19142..19723)
                     /locus_tag="ACHOSM_RS16225"
                     /old_locus_tag="pgaptmp_003245"
     CDS             complement(19142..19723)
                     /locus_tag="ACHOSM_RS16225"
                     /old_locus_tag="pgaptmp_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU4559709.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            complement(19701..20897)
                     /gene="yaiP"
                     /locus_tag="ACHOSM_RS16230"
                     /old_locus_tag="pgaptmp_003246"
     CDS             complement(19701..20897)
                     /gene="yaiP"
                     /locus_tag="ACHOSM_RS16230"
                     /old_locus_tag="pgaptmp_003246"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGU4559710.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQFFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            complement(20907..21577)
                     /locus_tag="ACHOSM_RS16235"
                     /old_locus_tag="pgaptmp_003247"
                     /pseudo
     CDS             complement(20907..21577)
                     /locus_tag="ACHOSM_RS16235"
                     /old_locus_tag="pgaptmp_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
BASE COUNT         5181 a         5637 c         5877 g         5140 t
ORIGIN      
        1 tcgcgaataa cccgacaagg aacgccagcc gccacgacgt ttggtggaat gtcttttgtg
       61 acgatactac tcgcgccaat aacagaatta tccccgatgg tgacgtctgg attaataacc
      121 acatgacttc cgatccagac gttattgcca atcgttatcg gaaaagagta catctcgccg
      181 ttttttctca attcatggtg tacagggtgt cccgtaacgg aaagagtaac gttgggtgca
      241 atcagtacgt tatcaccgat tgttaccgtg tagtcatcga caatggttaa attgaaattt
      301 gcataaaaat tgcggcctat atggatgttg gaaccgtaag agaaatagac aggcggttct
      361 acccaggcgt tttcccctac cgtggcaaac atttctttaa tcaggctttc tcttttttca
      421 acttctgatg gatgcgagtg attaaactca tacattaacg ttttcccacg aagtcttttt
      481 tccggtaagc cttcgcacat atcggtaaat agcttgcctg ctcttattct ttcggtcatt
      541 ggcatgttca atgcgatcac tccgttatga tatgttggtc ggataaggcg ctcgcgccgc
      601 atccgacatt gattgcttaa gcgacttcat tcacctgacg acgcagcagg gaaagcgggc
      661 cggggccgct aagcgtgaac acggaaatta aggtgaagcc cagcgccacc agacccagca
      721 ccagataagc gccctggaaa ccgatgcttt catacatatt gcccgccagt acagacataa
      781 aaatcatcgc cagttgctta aagaagcaga aacagaccag ataaatcgtc gctgaaaaac
      841 gcacttcaaa ctggctggta atatatttaa agcagcccac cagcaggaac ggtacttcaa
      901 acatatgcag cgttttcaga ataaccactt ccagcgctga ggtggcgaac gatgagccaa
      961 taatacgtac agacataata gtgccagcca gcagcagggc atttttccca ccgatgcgat
     1021 taatgatcag tggcgcaaag aacataatcg aggcgttaag taattcgccc attgtcgtta
     1081 cgtagccaaa tacccgcgta ccctgttcac cggtggcaaa gaaagaagta aagaaattag
     1141 caaactgttg gtcaaaaaca tcgtaggtgc aggaaacgcc aataacatac agtgacaaaa
     1201 accacagttt tggctgtctg aacagttcca gcgccagctt aaggctaaat gccgaatggt
     1261 tggcacctac cgcattggca accgtggcgg aagagggcgc atccgttttg gcgaaaaaga
     1321 gtaaaacggc gaggatgagt gcacagccag aacccagcca gaaaacaaac tgattattga
     1381 tggtgaacat gatgccgaca atcgaggcac acaacgccca gccaacacag ccaaacatcc
     1441 gcgcgcgacc aaattcgaaa ttactgcgac ggctgacttt ctcaataaat gcctctactg
     1501 ctggcgcacc ggcgttaaaa caaaagccta gataaatacc accaacaatc gatcctacta
     1561 aaatgttgta ttgtaacagt ggcccgaaga taaaaataaa gaacggagca aacatcacta
     1621 acatgccggt aataatccac agcaggtttt tgcgcagccc gagtttgtca gaaagcagac
     1681 caaacagcgg ttggaataat agcgagaaca gagaaatagc ggcaaaaata atacccgtat
     1741 cacttttgct gatatggttg atgtcatgta gccaaatcgg gaaaaacggg aagtaggctc
     1801 ccatgataaa aaagtaaaag aaaaagaata aaccgaacat ccaaaagttt gtgtttttta
     1861 aatagtacat aatggatttc cttacgcgaa atacgggcag acatggcctg cccggttatt
     1921 attatttttg acaccagacc aactggtaat ggtagcgacc ggcgctcagc tggaattccg
     1981 ccgatactga cgggctccag gagtcgtcgc caccaatccc catatggaaa ccgtcgatat
     2041 tcagccatgt gccttcttcc gcgtgcagca gatggcgatg gctggtttcc atcagttgtt
     2101 gttggctgta gcggctgatg ttgaactgga agtcgccgcg ccactggtgt gggccataat
     2161 tcaattcgcg cgtcccgcag cgcagaccgt tttcgctcgg gaagacgtac ggggtataca
     2221 tgtctgacaa tggcagatcc cagcggtcaa aacaggccgc agtaaggcgg tcgggatagt
     2281 tttcttgcgg ccctaatccg agccagttta cccgctctgc gacctgcgcc agctggcagg
     2341 tcaggccaat ccgtgccgga tgcggtgtat cgctcgctac ttcaacatca acggtaatcg
     2401 ccatttgacc actaccatca atccggtagg ttttccggct gataaataag gtttttccct
     2461 gatgctgcca cgcgtgagcg gtcgtaatca gcaccgcgtc ggcaagtgta tctgccgtgc
     2521 actgcaacaa cgctgcttcg gcctggtaat ggcccgccgc cttccagcgt tcgacccagg
     2581 cgttagggtc aatgcgggtc gcttcactta cgccaatgtc gttatccagc ggtgcacggg
     2641 tgaactgatc gcgcagcggc gtcagcagtt gtttttcatc gccaatccac atctgtgaaa
     2701 gaaggcctga ctgacggtta aattgccaac gcttattacc cagctcgatg caaaaatccg
     2761 tttcgctggt ggttagttgc gggatgatat gggacgcgga ggggtgcgtc acactgaggt
     2821 tttccgccag acgccactgc tgccaggcgc tgatgtgccc ggcttctgac catgcggtcg
     2881 cgttcggttg cactacgcgt actgtgagcc agagttgccc ggcgctctcc ggctgcggta
     2941 gttcaggcag ttcaatcaac tgtttacctt gtggagcgac atccagaggc acttcaccgc
     3001 ttgccagcgg cttaccatcc agcgccacca tccagtgcag gagctcgtta tcgctatgac
     3061 ggaacaggta ttcgctggtc acttcgatgg tttgcccgga taaacggaac tggaaaaact
     3121 gctgctggtg ttttgcttcc gtcagcgctg gatgcggcgt gcggtcggca aagaccagac
     3181 cgttcataca gaactggcga tcgttcggcg tatcgccaaa atcaccgccg taagccgacc
     3241 acgggttgcc gttttcatca tatttaatca gcgactgatc cacccagtcc cagacgaagc
     3301 cgccctgtaa acggggatac tgacgaaacg cctgccagta tttagcgaaa ccgccaagac
     3361 tgttacccat cgcgtgggcg tattcgcaaa ggatcagcgg gcgcgtctct ccaggtagcg
     3421 aaagccattt tttgatggac catttcggca cagccgggaa gggctggtct tcatccacgc
     3481 gcgcgtacat cgggcaaata atatcggtgg ccgtggtgtc ggctccgccg ccttcatact
     3541 gcaccgggcg ggaaggatcg acagatttga tccagcgata cagcgcgtcg tgattagcgc
     3601 cgtggcctga ttcattcccc agcgaccaga tgatcacact cgggtgatta cgatcgcgct
     3661 gcaccattcg cgttacgcgt tcgctcatcg cgggtaacca gcgcggatca tcggtcagac
     3721 gattcattgg caccatgccg tgggtttcaa tattggcttc atccaccacg tacaagccga
     3781 agcggtcgca cagcgtgtac cacagcggat ggttcggata atgcgaacaa cgcacggcgt
     3841 taaagttgtt ctgcttcatc agcaggatat cctgcaccat cgtctgctca tccatgacct
     3901 gaccatgcag aggatgatgc tcgtgacggt taacgccgcg aatcagcaac ggcttgccgt
     3961 tcagcagcag cagaccattt tcaatccgca cctcgcggaa accgacatcg caggcttctg
     4021 cttcaatcag cgtgccgtcg gcggtgtgca gttcaaccac cgcacgatag agattcggga
     4081 tttcggcgct ccacagtttc gggttttcga cgttcagacg tagtgtgacg cgatcggcat
     4141 aaccaccacg ctcatcgata atttcaccgc cgaaaggcgc ggtgccgctg gcgacctgcg
     4201 tttcaccctg ccacaaagaa accgtcaccc gcagctcatc gcgcagctcg ccgtacatct
     4261 gaacttctgc ctccagtacc gcgcggctga aatcatcatt aaagtgagtg gcaacatgga
     4321 aatcgctgat ttgtgtgctc ggtttatgca gcaacgagac gtcacggaaa atgccgctca
     4381 ttcgccacat atcctgatct tccagataac taccgtcact ccagcgcagc accatcaccg
     4441 cgaggcggtt ttctccggcg tgtaaaaagg cgctcaggtc aaattcagac ggcaaacgac
     4501 tgtcctggcc gtaaccgacc cagcgcccgt tgcaccacag atgaaacgcc gagttaacgc
     4561 catcaaaaat aattcgcgtc tggccttcct gtagccagct ttcatcaaca ttaaatgtga
     4621 gcgagtaaca acctgtcgga ttctccgcgg gaacaaacgg cggattgacc gtaatgggat
     4681 aggtcacgtt ggtgtagatg ggcgcatcgt aaccgtgcat ctgccagttt gaggggacga
     4741 cgacagtatc ggcctcagga agatcgcact ccagccagct ttccggcacc gcttctggtg
     4801 ccggaaacca ggcaaagcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     4861 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     4921 tcagttgggt aacgccaggg ttttcccagt cacgacgttg taatacgacg accagtgaat
     4981 ccgtaatcat agtcatagct gtatcctgtg taaaattgtt atccgctcac aatttcacac
     5041 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     5101 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     5161 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt
     5221 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
     5281 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
     5341 ggttaacggc gggatataac acgagctgtc ttcggtatcg tcgtatccca ctaccgagat
     5401 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg
     5461 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg
     5521 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt
     5581 gcgagtgaga tatttatgcc agcccgccag acgcagacgc gccgagacag aacttaatgg
     5641 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
     5701 cgtaccgtct tcatgggaga aaataatact gttgatggga gtctggtcag agacatcaag
     5761 aaataacgcc ggaacattag cgcaggctgc ttccacagaa gtggcatcct ggtcatccag
     5821 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt
     5881 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
     5941 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
     6001 gacaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
     6061 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
     6121 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc
     6181 gtatagcgtt actggtttca cattcatcac cctgaattga ctctcttccg ggcgctatca
     6241 tgccataccg cgaaaggttt tgcgccattc gatggtgtca acgtaaatgc acgccgcttc
     6301 gccgtccggc caccagaata gcctgcgatt caaccccttc ttcgatctgt tttgctaccc
     6361 gttgtagcgc cggaagatgc ttttccgcgg cctgttcaat ggtcattgcg ctcgccatat
     6421 acaccagatt cagacagcca atcacccgtt gttcactgcg cagcggtacg gcgatagagg
     6481 cgatcttctc ctcctgatcc cagctgcggt agttctgtcc gtaaccctct ttgcgcgcgc
     6541 gcgccagaat ggcttgcagc tttaacggtt ctcgtgccag ttgatagtca tcaccggggc
     6601 gggaggctaa catttcgatt aattccttgc gttcttgttc cgggcaaaag gccagccagg
     6661 tcaggcccga ggcggttttc agaagcggca aacgtcgccc gaccattgcc cggtgaaaag
     6721 ataagcggct gaaacggtgg gtggtttcgc gtaccaccat tgcatcaaca tccagcgtgg
     6781 acacatctgt cggccatacc acttcgcgca acagatcgcc cagcagtggg gccgccagtg
     6841 cagaaatcca ctgttcgtca cgaaatcctt cgcttaattg ccgcactttg atggtcagac
     6901 gaaaactgtc atcggagggg ctacggcgga catatccctc ttcctgcagc gtctccagca
     6961 gtcgccgcac agtggtgcga tgcaggccgc tgagttccgc cagcagcccg acgctggcac
     7021 cgccatcaag tttatttaac atatttaata acattagacc gcgggttaag ccgcgcacgg
     7081 ttttgtattc cgtctgctca ttgttctgca tattaattga catttctata gttaaaacaa
     7141 cgttgtgcac ctggtgcaca ttcgggcatg ttttgattgt agccgaaaac acccttctta
     7201 tactgagcgc acaataaaaa atcatttaca tgtttttaac aaaataagtt gcgctgtact
     7261 gtgcgcgcaa cggcattttg tccgagtcgt gaggtactga aatggcaata caacaccctg
     7321 acatccagcc tgctgttaac catagcgttc aggtggcgat cgctggtgcc ggtccggtcg
     7381 ggctgatgat ggcgaactat ctcggtcaga tgggcattga cgtgctggtg gtggagaaac
     7441 tcgataagtt gatcgactac ccgcgtgcga taggtattga tgacgaggcg ctgcgcacca
     7501 tgcagtcggt tggcctggtc gataatgttc tgccgcacac tacaccgtgg cacgcgatgc
     7561 gttttctcac cccgaaaggc cgctgttttg ctgatattca gccaatgacc gatgaatttg
     7621 gctggccgcg ccgtaacgcc tttattcagc ctcaggtcga tgcggtgatg ctggaagggt
     7681 tgtcgcgttt tccgaatgtg cgctgcttgt ttgcccgcga gctggaggcc ttcagccagc
     7741 aaaatgacga agtgaccttg cacctgaaaa cggcagaagg gcagcgggaa acggtcaaag
     7801 cccagtggct ggtagcctgt gacggtggag caagttttgt ccgtcgcact ctgaatgtgc
     7861 cgtttgaagg taaaactgcg ccaaatcagt ggattgtggt agatatcgcc aacgatccgt
     7921 taagtacgcc gcatatctat ttgtgttgtg atccggtgcg cccatatgtt tccgccgcgc
     7981 tgcctcatgc ggtacgtcgc tttgaattta tggtgatgcc gggagaaacc gaagaacagc
     8041 tgcgtgagcc gcaaaatatg cgcaagctgt taagcaaagt gctgcctaat ccggacaatg
     8101 ttgaattgat tcgccagcgt gtctacaccc acaacgcgcg actggcgcaa cgtttccgta
     8161 ttgatcgcgt actgctggcg ggcgatgccg cgcacatcat gccggtatgg caggggcagg
     8221 gctataacag tggtatgcgc gacgccttta acctcgcatg gaaactggcg ttggttatcc
     8281 aggggaaagc ccgcgatgcg ctgctcgata cctatcaaca agaacgtcgc gatcacgcca
     8341 aagcgatgat tgacctgtcc gtgacggcgg gcaacgtgct ggctccgccg aaacgctggc
     8401 agggtacgtt acgtgacggc gtttcctggc tgttgaatta tctgccgcca gtaaaacgct
     8461 acttcctcga aatgcgcttc aagccgatgc cgcaatatta cggcggtgcg ctgatgcgtg
     8521 agggcgaagc gaagcactct ccggtcggca agatgtttat tcagccgaaa gtcacgctgg
     8581 aaaacggcga cgtgacgctg ctcgataacg cgatcggcgc gaacttcgcg gtaattggct
     8641 ggggatgcaa tccactgtgg gggatgagcg acgagcaaat ccagcagtgg cgcgcgttgg
     8701 gcacacgctt cattcaggtg gtgccggaag tgcaaattca taccgcacag gataaccacg
     8761 acggcgtact acgcgtgggc gatacgcaag gtcgcctgcg tagctggttc gcgcaacaca
     8821 atgcttcgct ggtggtgatg cgcccggatc gctttgttgc cgccaccgcc attccgcaaa
     8881 ccctgggcaa gaccctgaat aaactggcgt cggtgatgac gctgacccgc cctgatgccg
     8941 acgtttctgt cgaaaaggta gcctgatatg cacgcttatc ttcactgtct ttcccactcg
     9001 ccgctggtgg ggtatgtcga cccggcgcaa gaggtgctcg atgaggtcaa tggcgtgatt
     9061 gccagcgccc gcgagcgtat tgcggcattc tcccctgaac tggtggtgct gtttgcgcca
     9121 gatcactaca acggcttttt ctatgacgtg atgccaccgt tctgtttagg cgttggagcg
     9181 acggcaattg gtgatttcgg cagtgcggca ggagagctgc ccgtgcctgt ggagctggcg
     9241 gaggcctgtg cgcatgccgt catgaagagc gggatcgatc ttgccgtttc ttactgtatg
     9301 caggtggacc acgggttcgc ccagccgctg gagttcctgc tcggtgggct ggataaggtg
     9361 ccagttctgc ctgtgttcat caacggtgtc gccacgccgc tgcccggttt ccagcgtacc
     9421 cgcatgttgg gtgaagccat tggacgtttc accagcactc tcaataaacg cgtgctgttc
     9481 ctaggttccg gtgggctttc ccatcagccg ccggtgcccg aactggcgaa agccgatgcc
     9541 catatgcgcg accgtctgtt ggggagcggg aaagatttac ccgccagtga gcgcgaattg
     9601 cgtcagcaac gggtgattag cgccgctgag aagtttgttg aggatcagag aacgctgcat
     9661 ccgctcaacc cgatttggga taaccagttc atgactttgc tggagcaggg acgcatacag
     9721 gaactggatg ccgtcagtaa cgaagagctt tccgccattg ccggaaagtc gacacatgaa
     9781 atcaaaacct gggtcgccgc ttttgccgct atttctgcgt ttggcaactg gcgtagcgaa
     9841 gggcgttatt accgcccaat cccggagtgg attgccggat ttggctcgtt aagcgccaga
     9901 acagagaact gaatatgcag gagaagatga tgagttatca gccacaaacc gaagccgcca
     9961 ccagccgttt tctgaatgta gaagaagcgg gtaaaacgct gcgcatccat tttaatgact
    10021 gcggacaagg cgacgaaacc gttgtcctgc tgcatggttc cggcccgggt gctactggct
    10081 gggcgaactt ccgccgcaat atcgatccgc tggtagaggc gggctatcgg gtgatcctgc
    10141 tggattgtcc gggttggggc aagagcgatt cgatcgttaa tagtggttcg cgatcggatc
    10201 ttaatgcacg aatcctgaaa agcgtggtgg atcaactgga tatcgccaaa atccacctgc
    10261 tgggcaactc gatggggggc catagttctg tggcgttcac ccttaactgg ccggagcgcg
    10321 tcggcaaact ggtgctgatg ggcggcggta cgggcggcat gagtttgttt acgccgatgc
    10381 caaccgaagg tattaagcga ctgaatcagc tttatcgtca gccgactatc gaaaacctga
    10441 agctgatgat ggatatcttc gtttttgata ccagcgattt gaccgacgcc ctgtttgaag
    10501 cgcgcctgaa taatatgctg tcgcgccgcg atcacctgga aaactttgtt aaaagcctgg
    10561 aagccaatcc gaaacagttc ccggattttg gcccacgtct ggcggaaatc aaagcgcaaa
    10621 ccctgattgt ctgggggcgc aacgaccgct ttgtgccgat ggatgcgggg ctgcgtctgc
    10681 tgtccggcat tgccggttct gaactgcata tcttccgcga ctgtggtcac tgggcgcagt
    10741 gggaacatgc cgacgctttc aatcaattgg tgctgaattt cctcgcacgc ccttaaggaa
    10801 tggtcatgac gaagcatact cttgagcaac tggcggcgga tttacgccgc gccgcagagc
    10861 agggcgaagc gattgcgccg ctgcgcgatc tgattggtat cgataacgct gaagcggcct
    10921 acgccattca gcacataaat gtgcaatatg acgttgtgca ggggcgtcgc gtggtagggc
    10981 gtaaagtggg cctgacgcat ccgaaagtgc aacaacaact gggcgttgat caaccggatt
    11041 ttgggacgtt atttgccgac atgtgttatg gcgataacga aatcattcct ttttcccgtg
    11101 ttctgcaacc ccgcattgaa gcggagatcg cactggtgtt gaaccgcgat ttacccgcaa
    11161 ctgatatcac cttcgacgaa ctgtataacg ccattgaatg ggtacttccg gcgctggaag
    11221 tggtggggag ccgcattcgc gactggtcga ttcagtttgt cgataccgtg gcagataacg
    11281 cttcctgcgg ggtgtatgtc atcggcggtc cggcgcaacg tccggcgggg ttagacctga
    11341 aaaactgcgc catgaagatg acgcgtaata acgaagaggt ttccagcggg cgcggcagcg
    11401 aatgcctggg acatccgctt aatgccgccg tctggctggc acgcaaaatg gccagtctgg
    11461 gtgaaccgct gcgcgccgga gatatcattc ttaccggggc attaggcccg atggtggcgg
    11521 tgaatgcggg cgatcgtttt gaagcccata ttgaaggcat aggttcagtt gctgcgacat
    11581 tttcaagcac agccccaaaa ggaagtctgt catgagtaag cgtaaagtcg ccattatcgg
    11641 ttctggcaac attggcaccg atctgatgat taaaattttg cgtcacggtc agcatctgga
    11701 gatggcggtg atggtcggca ttgatcctca gtccgacggt ctggcgcgtg ccagacgtat
    11761 gggcgtcgcc accacccatg aaggggtgat cgggctgatg aacatgcctg aatttgctga
    11821 tatcgacatt gtatttgatg cgaccagcgc gggcgcgcat gtgaaaaacg atgccgcttt
    11881 acgcgaagcg aaaccggata ttcgcttaat tgacctgacg cctgctgcca tcggccctta
    11941 ctgcgtgccg gtggttaacc tcgaggcgaa cgtcgatcaa ctgaacgtca acatggtcac
    12001 ctgcggcggc caggccacca ttccaatggt ggcggcagtt tcacgcgtgg cgcgtgttca
    12061 ttacgccgaa attatcgctt ctatcgccag taaatctgcc ggacctggca cgcgtgccaa
    12121 tatcgatgaa tttacggaaa ccacttcccg agccattgaa gtggtgggcg gcgcggcaaa
    12181 agggaaggcg attattgtgc ttaacccagc agagccaccg ttgatgatgc gtgacacggt
    12241 gtatgtattg agcgacgaag cttcacaaga tgatatcgaa gcctcaatca atgaaatggc
    12301 tgaggcggtg caggcttacg taccgggtta tcgcctgaaa cagcgcgtgc agtttgaagt
    12361 tatcccgcag gataaaccgg tcaatttacc gggcgtgggg caattctccg gactgaaaac
    12421 agcggtctgg ttggaggtcg aaggcgcagc gcattatctg cctgcctatg cgggcaacct
    12481 cgacattatg acttccagtg cgctggcgac agcggaaaaa atggcccagt cactggcgcg
    12541 caaggcagga gaagcggcat gaacggtaaa aaactttata tctcggacgt cacgttgcgt
    12601 gacggtatgc acgccattcg tcatcagtat tcgctggaaa acgttcgcca gattgccaaa
    12661 gcactggacg atgcccgcgt ggattcgatt gaagtggccc atggcgacgg tttgcagggt
    12721 tccagcttta actatggttt cggcgcacat agcgaccttg aatggattga agcagcggcg
    12781 gatgtggtga agcacgccaa aatcgcgacg ttgttgctgc caggaatcgg cactattcac
    12841 gatctgaaaa atgcctggca ggctggcgcg cgggtggttc gtgtggcaac gcactgtacc
    12901 gaagctgatg tttccgccca gcatattcag tatgcccgcg agctcggaat ggacaccgtt
    12961 ggttttctga tgatgagcca tatgaccacg ccggagaatc tcgcaaagca ggcaaagctg
    13021 atggaaggct acggtgcgac ctgtatttat gtggtggatt ctggcggtgc gatgaacatg
    13081 agcgatatcc gtgaccgttt ccgcgccctg aaagcagagc tgaaaccaga aacgcaaacc
    13141 ggcatgcacg ctcaccataa cctgagtctt ggcgtggcga actctatcgc ggcggtggaa
    13201 gagggctgcg accgaatcga cgccagcctc gcgggaatgg gcgcgggcgc aggtaacgca
    13261 ccgctggaag tgtttattgc cgccgcggat aaactgggct ggcagcatgg gaccgatctc
    13321 tatgcgttaa tggatgccgc cgacgacctg gtgcgtccgt tgcaggatcg tccggtacga
    13381 gtcgatcgcg aaacgctggc gctgggatac gctggtgttt actcgagctt cctgcgtcac
    13441 tgtgaaacgg cggcggcgcg ttatggctta agtgcggtgg atattctcgt tgagctgggc
    13501 aaacgccgga tggttggcgg ccaggaggat atgatcgtcg acgtggcgct ggatctgcgc
    13561 aacaacaaat aatgatgact gccgataacg tgcattttgt atgcccatcc cagcacgaca
    13621 gccggatgcg gcgtgaacgc cttatccggc ctacatttcg ctacatttcg cccgtaggca
    13681 gtcattaaat agttctgatt acgcggctgg caatgtatca gtcgcgattc acctgcactc
    13741 gcaatgaggt tcatcttatg tcgactcgta ccccttcatc atcttcatcc cgcctgatgc
    13801 tgaccatcgg gctttgtttt ttggtcgctc tgatggaagg gctggatctt caggcggctg
    13861 gcattgcggc gggtggcatc gcccaggctt tcgcactcga taaaatgcaa atgggctgga
    13921 tatttagcgc cggaatactc ggtttgctac ccggcgcgtt ggttggcggg atgctggcag
    13981 accgttatgg tcgtaagcgt attttgattg gctcagttgc gctgtttggt ttgttctcac
    14041 tggcaacggc gattgcctgg gatttcccct cactggtctt tgcgcggctg atgaccggtg
    14101 tcgggctggg ggcggcgttg ccgaatctta tcgccctgac atctgaagcc gcgggtccac
    14161 gttttcgtgg gacggcagtg agcctgatgt attgcggtgt tcccattggc gcggcgctgg
    14221 cggcgacact gggtttcgcg ggggcaaact tagcatggca aacggtgttt tgggtaggtg
    14281 gtgtggtgcc gttgattctg gtgccgctat taatgcgctg gctgccggag tcagcggttt
    14341 tcgctggcga aaaacaggct gcgccaccac tgcgtgcctt atttgcgcca gaaacggcaa
    14401 ccgcgacgct gctgctgtgg ttgtgttatt tcttcactct gctggtggtt tatatgttga
    14461 tcaactggct accgctactt ttggtggagc aaggattcca gccatcgcag gcggcagggg
    14521 tgatgtttgc cctgcaaatg ggggcggcaa gcgggacgtt aatgttgggc gcattgatgg
    14581 ataagctgcg tccagtaacc atgtcgctac tgatttatag cggcatgtta gcttcgctgc
    14641 tggcgcttgg aacggtgtcg tcatttaacg gtatgttgct ggcgggattt gtcgcggggt
    14701 tgtttgcgac aggtgggcaa agcgttttgt atgccctggc accgttgttt tacagttcgc
    14761 agatccgcgc aacaggtgtg ggaacagccg tggcggtagg gcgtctgggg gctatgagcg
    14821 gtccgttact ggccgggaaa atgctggcat taggcactgg cacggtcggc gtaatggccg
    14881 cttctgcacc gggtattctt gttgctgggt tggcggtgtt tattttgatg agtcggagat
    14941 caagaatgca gccgtgtgcc gatgcctgac gcgccttatt aagcaggggc gaaagggact
    15001 tgcccccaat acacccacgg cgtttacagg tatactcgct aaaaattatt cagcgggttt
    15061 ggaaacaaag atggcaaaac ttaccttaca agagcagttg ctcaaagcag gattagtcac
    15121 cagcaaaaaa gcggcgaagg tcgagagaac ggcgaaaaaa tcgcgcgttc aggcgcgtga
    15181 agctcgggct gcggtagaag aaaataaaaa ggcacagctt gagcgtgata aacagcttag
    15241 cgaacagcaa aaacaagcgg cgttggcgaa agaatataaa gcccaggtga agcagctcat
    15301 tgaaatgaac cgaatcacca ttgccaatgg cgatattggt tttaacttca ctgacggcaa
    15361 tctgattaag aagatttttg tcgataagct cacacaggcg cagttgatta atggtcgtct
    15421 ggcgattgcc cgcttgttgg tcgataacaa tagcgaaggt gaatacacca ttattcccgc
    15481 cagtgttgcc gataaaattg ctcagcgtga tgccagcagt attgtgttgc acagcgcgct
    15541 cagtgccgaa gagcaggatg aagacgatcc gtatgccgac ttcaaagtgc ctgatgattt
    15601 gatgtggtaa acatagataa gtcgtgcgca gatgcctgat gcgacgctat gcgcgtctta
    15661 tcaggcctac ggtttatggg cgaagtgtag accggataag gcgttcacgc cgcatccggc
    15721 agtcgtgcgc catgcctgat gcgacgcttt gcgcgtctta tcaggcctac ggtttacggg
    15781 cgaagtgtag gccggataag gcgttcacgc cgcatccggc agtcgtgcac tattatcaac
    15841 gcatattcag tttattggcg tggtaggcaa tatgctcgcc aataaaactg gagacaaaat
    15901 aatagctgtg atcataaccc tcgtgataac ggattaacgt cttgatattc atctcctggc
    15961 agatcttttc aagatttgga gtccgcagct gttctgcgta aaaatcatca ctcaccccct
    16021 gatcaaccat gatttccgca acgcgttgac cttgtgaaat aagactcacc gggtcgtaat
    16081 ccaaccaggc atctttattt tcagcaagat atgcagcaaa ggcttgctgt ccccacggca
    16141 cttgcgatgg ggagacaatg ggcgaaaacg ccgagacgct gacatattca tctgggttac
    16201 gtaacgccag caccagcgcg cccagcccgc ccatagaatg accagagata gactttttgg
    16261 ccgttgccgg aaaatgatgc atcactaaat ccggcagctc gttgcggata tagtcataca
    16321 ttttgtaatg ttcattccac ggcgcttgcg tcgcgttcag gtaaaacccg gcaccttgcc
    16381 cgagatcgta acggtcagca tctgcgacat gactgcctcg cggactggtg tccggcgcaa
    16441 caacaataat gttgtgctca gccgcgtaac gctgcatccc cgatttagta atgaaattct
    16501 gctcgttgca ggtcaggcct gaaagccagt acagcaccgg caatttttca ttcgcggctt
    16561 ttggtgggag atagacgccg acattcattt cacatttcag tgattgggaa taatgccgat
    16621 acacattttg ccagccgcca aagctggcat gtttttcaat gagttccatc agtcattatc
    16681 tcacggttaa tcccccggac ggctgcccgg ggagagtatt aagaaccccc gcctgaactg
    16741 gacggggtaa acctgcggga aatcagtaac gaattacggt tcgaatggat ttgcctccat
    16801 gcatcaggtc gaaggcgtca ttaatttcat ccaggctcat ggtatgcgtg acaaacggtt
    16861 ccagatcgat atcacctttc atcgcatctt caaccatatc cggtaactgg gaacgacctt
    16921 tcacgccgcc aaacgcggaa cctttccata cgcgaccggt gaccaactgg aatggtcggg
    16981 tggagatttc ctgaccagaa ccggcgaccc cgatgatcac cgactgaccc cagccgcggt
    17041 gcgcactttc cagcgccgca cgcatcacgt tgacgttacc gatgcattca aaggtatggt
    17101 cgatacccca tttgttgata tccagcagga catctttaat cggtttgtcg tagtcattcg
    17161 ggttaatgca gtcggtggca ccgaagcgac gcgccagatc gaatttcttc gggttcgtat
    17221 caatagcgat aatccgaccc gctttcgcct gacgcgcgcc ctgaaccact gccagaccaa
    17281 tcgcgccaag accaaacacg gcaacagaat cacctggctg gactttagct gtgttgtgta
    17341 ccgcgccaat accggtggtc acgccacagc ccagcaggca gacgtgttca tggtttgctt
    17401 ctggattaat ttttgccaga gacacttccg cgaccacggt gtattcactg aatgttgagc
    17461 atcccatgta gtgataaagc ggctgcccgt tgtaagaaaa acgggtggtg ccgtctggca
    17521 tcaagccttt accctaggtt tcgcgaaccg caacacagag gttagttttg ccagaacgac
    17581 agaactcaca ctcgccgcac tccgcggtgt aaagcgggat cacatggtcg ccaggtttga
    17641 cgctggttac gccttcaccg acttcaacca caacgccggc cccttcgtga ccgagaacca
    17701 ccgggaatac accttccggg tcatccccgg agagggtaaa tgcgtcggta tggcaaacgc
    17761 cggtatgggt gactttaatc agcacttcac cttttttcgg tggtgcaacg tcaatttcaa
    17821 cgatttccag cggtttaccg ggagcaaatg caacggcagc acgtgatttc atctctcgct
    17881 cttcctcaat atggtaatag attcagctat ttaagatagg ctcgaaccag ttcaatagtg
    17941 tcgtcaacgg attggctgac ttcgcggctg tagcagtcat ttcggtcaaa cgtttcccgg
    18001 atatggcttt caagcacttc tgccatcagc ccattagccg cgccccgaac ggcagcgatc
    18061 tgttggagta tggcacggca ttcggcatca ccctccagcg accgttccag agcatcaatc
    18121 tgccctcgaa tacgacgaac tcgagtaagg acctttttct tctcttccgg agtactgggc
    18181 atttcgcacc tcattatctg cacgcaatat actatagggg ggtatgctat atgtcaatgc
    18241 atacccccct atagtatata acactggaga ataaaattta tccggtgaat gtggtcggaa
    18301 aacaaagagg aaaggggggg ctaatcggca gggaaggccg ccccggatag cgggcggcag
    18361 aaggaatcag aatttccagg tcagatgggc tgcaagttgc agaccgttat aatcatcggt
    18421 tggggtgtcg taccacactt tacctgccgt cagcccgaga ttaagttgct cagtcagcgg
    18481 ttgaatacgt tgcagactga cgctcttcat gctgccgtag cgtgtttctg gcgtccagtc
    18541 ataggtgtaa gcgcctgttc cgcggcttag ccatagttgc gtataaccag tgccgcgcgg
    18601 gtcattcaga cgcacggaaa tcatattgct ataactacca cctgcatcgc tggagtcata
    18661 atgggtatag cggtagctgg tgatcaccgg gccagtatag agtgatacgc cgccttgcca
    18721 ggcatcgact tcgacatcat cgtaatattt agtgtagcga taaccggtcg tgaaaagcgt
    18781 ttttggcaac agggccaagt taatatcctg acgaaaatcc tggcgggcaa agaccggcgt
    18841 attatccgca aaggcaatac ccgttcgggt tgtcagccag ttattccatt tataccagac
    18901 cgcaccctga ccgcgagtgg cattgaaatg ttcagtttca taatcacgac gcccctggga
    18961 aagattaaaa agcagcgttg cgttttcaac tttcgccacc agttcagcat acgctaaatt
    19021 acggttgcca tgatcgccag aataatcggt aaaatcgtag cccgcggtaa tggaagttaa
    19081 cccggcataa gcgggcaatg cgctaaaaat ggccgccgct aataacgtcc gttttatcat
    19141 tttaatctcc tgtacggata agttcttgtc ggagtgaata agttccggca ggtaaattac
    19201 gttctacaat cacgcgcggg ccaagttgcg tgttcgggga aataattcgc ccaggcaaaa
    19261 ttataacttg tacgccaagg cgtgaacgct gcccgatata acaacctaat ttatcgcatc
    19321 cggtagcgat aattccctct ggagttcgaa cagacacagg ctgttcatcc agacgatgat
    19381 tactggtacg tacttgtgcg cccaaatatg cctggtttgc aactaccgag tcggcaataa
    19441 aacattgcgg accaatcgtt gcttccgctt caataaccgc atttttaatt tcggtggcaa
    19501 aaccaatttt tacaccattg ctgattattg tgccaggacg aataaacgca taattaccta
    19561 tcaggcagtt tgcgccaatc actaccggac cctgaataac ggcaccatga caaatacggg
    19621 tattcgcgcc gataacaact tcacctgcag tttcatcaat aattacctgg tctgcaatct
    19681 gaatattttt ctctttaaca ttaagcttca gatagttttt cagttcctgt gacagaaggt
    19741 tgggaataag cggttgacgc ttccaccagt gcggaatagc gggtgggttt gtcgcgctga
    19801 ggttcgcgtc cagtaaaaaa ggcaataagt ccataaataa tccagatggc ataagctaat
    19861 aatacataca caacggaaag cggcgctaaa ggcaccaaca accagcaacg atgaaaccag
    19921 gcgctaaaag caccaataac acaaactacg ccgatccaga taagcggaaa cattgccaac
    19981 accacaccat gcggcccggt ggtgatgaat tcattaaacc aggtagtgag ataaatccca
    20041 acgccataaa tcacaaccaa cagcatagga aatatactga agataccgaa gcggctaaat
    20101 aaaagtcttt tatgcaggcg catacagacc gcgtatccca caatccaacg ccgccagcga
    20161 cgccactcct cacgcgggct gttgcattcc tgtgggtata cgatgcagcg attcgcctgc
    20221 cgaatacggt agccgtttgc cactaatgtc caggtgagat caaggtcttc gactttagta
    20281 cgatccgaga aaccgaactt acgcaataca tcagtacgga acatcccgca ggcaccgctg
    20341 ataataaacg gtgcgccgcc caggagctgc tgtagcgtgc gcttcataac aatcatcggc
    20401 aactttacgg tcgcgcggat gtgcggtaac agccccgcgc ctttcaacgc agtagaggga
    20461 atgccgccta cggcatcagc accgcgctca atttctgcca gcatatagcc cattccgtct
    20521 tgatcgggcg gaacataggt gtcggcatca cttaaaaaaa actggtcgca ggtggcataa
    20581 ttgaggccat tcatcagcgc accaccttta ccggtatttt tttgcgtgac ggcaacaaag
    20641 cggtcgcccc atttgcgttt gacttccgcc atgaccgctt cggtattgtc cgtggagccg
    20701 tcgttaacgc aaattacccg gcaaaaataa gggttacgca gtagattatc cagtgactgc
    20761 gccagacacg ggccttcgtt atacgcaggt ataatggcgt caatacagcc tttcttttga
    20821 ctgggtttac gtcttagcgt acttaaaaac catagcaaca ttgctatgga catacagata
    20881 aatatccagg ttttcatgca atttgctcaa agaatcattt tatgacttac aaagccttca
    20941 cccagatcgc tattgacctg ttgcccgcga aattgtgcaa ctgcacgcag gcgatcatga
    21001 cgcatataat cacgtcgttc ctgcttttat gtttttttaa tgcagcaagt ttgactgtga
    21061 aatattcttc tttaacggat tcaaaaacct gaggcataaa tgaaagccag gtacttgggg
    21121 tttcgtagcc gagaatttgt ggaatagtgc ggcaggcaac cattgaagct tgataaacag
    21181 cgagatgatc ctgatggcgg tcggcatcat gcatggtata tacccgcatg atttcaacat
    21241 cagaaggaat ttgattttta atgatgtctt ccagggcgga aatcatatca ttgagctgta
    21301 aatgagcgcg ggtgtcagca aaattaagat gaatagtttg gtggcaccct aatatcttta
    21361 gggcgttgcg cgattcttca tggcgatcga ttattccatc tgtaccagag ttgccggtag
    21421 tcatcaccac tgctgcgata taaattcctt tttgcgcaag acgagcaagc gatgcgccac
    21481 agcctaattc tatatcgtca ggatgtgcgc caatagcaag gatacccttt cttttatttg
    21541 ccgaagaaag gagggctgaa tctaaaacct tatccactta atgacactcc attttattta
    21601 ttatactaca agcacaacga tgcactcaga gacgtaatct ctggcgcagc cagatgttaa
    21661 tttataaatg ttaaatgttg ctaataactg aaaagcaagg gctttcagac ccggaggttc
    21721 gactgggtaa tgtttttatg caattcatat gttaagtgtt tgtatgtttg gtatgtatag
    21781 ttattttgtt ttatacattg tatttagatg tgatttagtt tgtcaattaa ttgca
//
LOCUS       DAZKHC020000072        21263 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZKHC020000072 DAZKHC020000000
VERSION     DAZKHC020000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.072"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            117..719
                     /gene="ais"
                     /locus_tag="ACHOSM_RS16240"
                     /old_locus_tag="pgaptmp_003248"
     CDS             117..719
                     /gene="ais"
                     /locus_tag="ACHOSM_RS16240"
                     /old_locus_tag="pgaptmp_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HGU4559711.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(758..1183)
                     /gene="nudI"
                     /locus_tag="ACHOSM_RS16245"
                     /old_locus_tag="pgaptmp_003249"
     CDS             complement(758..1183)
                     /gene="nudI"
                     /locus_tag="ACHOSM_RS16245"
                     /old_locus_tag="pgaptmp_003249"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HGU4559712.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1462..2004
                     /gene="yfaZ"
                     /locus_tag="ACHOSM_RS16250"
                     /old_locus_tag="pgaptmp_003250"
     CDS             1462..2004
                     /gene="yfaZ"
                     /locus_tag="ACHOSM_RS16250"
                     /old_locus_tag="pgaptmp_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HGU4559713.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2104..3306
                     /gene="yfaY"
                     /locus_tag="ACHOSM_RS16255"
                     /old_locus_tag="pgaptmp_003251"
     CDS             2104..3306
                     /gene="yfaY"
                     /locus_tag="ACHOSM_RS16255"
                     /old_locus_tag="pgaptmp_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HGU4559714.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLLQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEILWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3526..4308
                     /gene="yfaX"
                     /locus_tag="ACHOSM_RS16260"
                     /old_locus_tag="pgaptmp_003252"
     CDS             3526..4308
                     /gene="yfaX"
                     /locus_tag="ACHOSM_RS16260"
                     /old_locus_tag="pgaptmp_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HGU4559715.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLCTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4323..5528
                     /gene="rhmD"
                     /locus_tag="ACHOSM_RS16265"
                     /old_locus_tag="pgaptmp_003253"
     CDS             4323..5528
                     /gene="rhmD"
                     /locus_tag="ACHOSM_RS16265"
                     /old_locus_tag="pgaptmp_003253"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HGU4559716.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTFSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            5585..6874
                     /gene="yfaV"
                     /locus_tag="ACHOSM_RS16270"
                     /old_locus_tag="pgaptmp_003254"
     CDS             5585..6874
                     /gene="yfaV"
                     /locus_tag="ACHOSM_RS16270"
                     /old_locus_tag="pgaptmp_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4559717.1"
                     /translation="MSTALLDAAVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6892..7695
                     /gene="yfaU"
                     /locus_tag="ACHOSM_RS16275"
                     /old_locus_tag="pgaptmp_003255"
     CDS             6892..7695
                     /gene="yfaU"
                     /locus_tag="ACHOSM_RS16275"
                     /old_locus_tag="pgaptmp_003255"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HGU4559718.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPVDLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7736..8638)
                     /gene="rpnE"
                     /locus_tag="ACHOSM_RS16280"
                     /old_locus_tag="pgaptmp_003256"
     CDS             complement(7736..8638)
                     /gene="rpnE"
                     /locus_tag="ACHOSM_RS16280"
                     /old_locus_tag="pgaptmp_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HGU4559719.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDEPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8832..10022)
                     /gene="glpC"
                     /locus_tag="ACHOSM_RS16285"
                     /old_locus_tag="pgaptmp_003257"
     CDS             complement(8832..10022)
                     /gene="glpC"
                     /locus_tag="ACHOSM_RS16285"
                     /old_locus_tag="pgaptmp_003257"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HGU4559720.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(10019..11278)
                     /gene="glpB"
                     /locus_tag="ACHOSM_RS16290"
                     /old_locus_tag="pgaptmp_003258"
     CDS             complement(10019..11278)
                     /gene="glpB"
                     /locus_tag="ACHOSM_RS16290"
                     /old_locus_tag="pgaptmp_003258"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HGU4559721.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(11268..12896)
                     /gene="glpA"
                     /locus_tag="ACHOSM_RS16295"
                     /old_locus_tag="pgaptmp_003259"
     CDS             complement(11268..12896)
                     /gene="glpA"
                     /locus_tag="ACHOSM_RS16295"
                     /old_locus_tag="pgaptmp_003259"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HGU4559722.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            13169..14527
                     /gene="glpT"
                     /locus_tag="ACHOSM_RS16300"
                     /old_locus_tag="pgaptmp_003260"
     CDS             13169..14527
                     /gene="glpT"
                     /locus_tag="ACHOSM_RS16300"
                     /old_locus_tag="pgaptmp_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HGU4559723.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            14532..15608
                     /gene="glpQ"
                     /locus_tag="ACHOSM_RS16305"
                     /old_locus_tag="pgaptmp_003261"
     CDS             14532..15608
                     /gene="glpQ"
                     /locus_tag="ACHOSM_RS16305"
                     /old_locus_tag="pgaptmp_003261"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU4559724.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(15650..15892)
                     /locus_tag="ACHOSM_RS16310"
                     /old_locus_tag="pgaptmp_003262"
                     /pseudo
     CDS             complement(15650..15892)
                     /locus_tag="ACHOSM_RS16310"
                     /old_locus_tag="pgaptmp_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            15887..15991
                     /locus_tag="ACHOSM_RS16315"
                     /old_locus_tag="pgaptmp_003263"
                     /pseudo
     CDS             15887..15991
                     /locus_tag="ACHOSM_RS16315"
                     /old_locus_tag="pgaptmp_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16071..16721
                     /gene="inaA"
                     /locus_tag="ACHOSM_RS16320"
                     /old_locus_tag="pgaptmp_003264"
     CDS             16071..16721
                     /gene="inaA"
                     /locus_tag="ACHOSM_RS16320"
                     /old_locus_tag="pgaptmp_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HGU4559725.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(16775..17029)
                     /gene="yfaE"
                     /locus_tag="ACHOSM_RS16325"
                     /old_locus_tag="pgaptmp_003265"
     CDS             complement(16775..17029)
                     /gene="yfaE"
                     /locus_tag="ACHOSM_RS16325"
                     /old_locus_tag="pgaptmp_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HGU4559726.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(17029..18159)
                     /gene="nrdB"
                     /locus_tag="ACHOSM_RS16330"
                     /old_locus_tag="pgaptmp_003266"
     CDS             complement(17029..18159)
                     /gene="nrdB"
                     /locus_tag="ACHOSM_RS16330"
                     /old_locus_tag="pgaptmp_003266"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU4559727.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTCYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERKLMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(18393..20678)
                     /gene="nrdA"
                     /locus_tag="ACHOSM_RS16335"
                     /old_locus_tag="pgaptmp_003267"
     CDS             complement(18393..20678)
                     /gene="nrdA"
                     /locus_tag="ACHOSM_RS16335"
                     /old_locus_tag="pgaptmp_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU4559728.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQHLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            20806..20949
                     /gene="ypaB"
                     /locus_tag="ACHOSM_RS16340"
                     /old_locus_tag="pgaptmp_003268"
     CDS             20806..20949
                     /gene="ypaB"
                     /locus_tag="ACHOSM_RS16340"
                     /old_locus_tag="pgaptmp_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HGU4559729.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4983 a         5176 c         5704 g         5400 t
ORIGIN      
        1 gcattttgcc gacatcgtag gatttttaat attacaccaa ctgctaatta tcgccagaaa
       61 tgtcgctcaa tttcacggta attgtctggt tgcgcttgtc tataggtgga gtttacgtgt
      121 tagctttttg ccgctcatca ttgaagtcaa aaaaatattt catcatttta ctggcgctcg
      181 ctgcaattgc cggactgggt actcatgccg cctggagtag caatggtttg ccacgtatcg
      241 acaataaaac attggccaga ctggcacagc agcacccggt tgtcgttttg tttcgccatg
      301 ctgaacgttg cgatcgttca accaatcaat gcttgtcaga taaaacaggt attacggtta
      361 aaggtaccca ggatgcccgt gaactgggca acgcttttag cgctgacatc cctgatttcg
      421 atctttattc cagtaatacc gtccggacca ttcagtcggc gacctggttt tccgcgggta
      481 aaaagctgac ggtagataaa cgacttcttc agtgcggtaa tgagatttat agtgcaatta
      541 aggacttaca aagcaaagcg cctgataaaa atatcgttat tttcacccat aatcattgcc
      601 tgacatatat tgctaaaaat aagcgtgatg cgacatttaa acctgattat ctggatggtt
      661 tagtcatgca tgtggaaaaa ggcaaagttt atctggatgg ggaattcgtt aaccactaaa
      721 ttttcaatct gacagccagt gatggctgtc atcattgtta aagaagacct ttcaaacgta
      781 acgtttttcg ggtggcaaca tttaaatcat aatgcaccag atcttcaggt tttacccacg
      841 cgtagtcctg aaactcttcg tttattttca cttctcggtt ggcagaaacg cagtcaaaaa
      901 tcaggtaaat catataaatc tcttccttgc gaccatctgc atacgtcttg gtgcgaatat
      961 catcgctgaa ggtccacggc gtgatttctg tcaaaagcag ctgttctccc agttcttcgc
     1021 gaatttcgcg gcgtagtgcc tcttcaattc gttcgccaga ctccacgccg ccacctgaaa
     1081 gcgcccattg accggggaaa acgccgcgat cgtcggccat tttgcacagc aaataagcac
     1141 catcattttg aatcaaaggg catacaatag tccgttgtcg cacaggggta ttccttataa
     1201 ataaaaagaa accatgagaa tacggtaact cagaaatatg tgaaagtgac agtaatctct
     1261 tttgcgatga taactgcact tattattatt tttgtgtgta tcggtaaagt gtgtttattt
     1321 tttcgtcgtt taattattta agaagtattt agttacaata agtgactaga tccctttttt
     1381 cgctcattga atcctctgtg ccaggcagta caatcccgca gctttgggaa ttttgattaa
     1441 ttgcttgtgg gaaaggggat aatgaaaaaa attgcgctag caggtctggc cggaatgctg
     1501 ttggtttctg catcggtcaa tgcaatgagc atcagcggtc aggcgggtaa agaatacacc
     1561 aatattggtg tcggttttgg gactgaatcg acgggcctgg cgttaagcgg taactggaca
     1621 cataacgacg acgacggtga tgtcgctggc gtggggctgg ggttgaatct gcctctaggg
     1681 ccgttaatgg cgaccgttgg cggaaaaggc gtttacacca acccgaatta cggcgatgaa
     1741 ggttatgccg cagcggtagg aggtggtttg cagtggaaaa ttggcaacag cttccgtttg
     1801 tttggcgagt attactactc tccggattca ctctccagcg gtattcaaag ttatgaggaa
     1861 gcgaatgctg gcgcgcgtta caccattatg cgtccagtca gtattgaggc gggttatcgc
     1921 tacctgaatt tgtcgggtaa agacggtaac cgcgacaacg ccgtggctga cggcccgtat
     1981 gttggggtta acgccagttt ctgatccctt ccggcgcggc attctgtcgc gccattcagt
     2041 ttcttactac gccttatctc cctttgcgtt atagtgattc gataacaaaa gcaggagata
     2101 accatgttaa aagtggaaat gttatccacc ggggatgaag tgttacacgg gcaaatcgtt
     2161 gacactaacg ctgcctggct ggccgatttt ttctttcatc aggggctgcc attatctcgc
     2221 cgcaatacgg tgggggataa ccttgatgac ttagtcacca ttcttcgcga acgtagtcag
     2281 cacgccgatg tgctgatcgt taacggcggg ctgggaccga ccagcgatga tttaagcgca
     2341 ctcgccgctg cgacagcaaa aggtgaaggc ctggtgctgc atgaggcctg gctcaaagag
     2401 atggaacgct atttccacga acgtggacga gtaatggcac cgagcaaccg taaacaagcg
     2461 gagctgcctg ccagtgctga atttatcaat aacccggtag gcaccgcctg tggttttgcc
     2521 gtgcagctta atcgttgcct gatgttcttt actcccggcg taccgtcaga atttaaggtg
     2581 atggtcgaac acgaaatcct gccgcgcctg cgcgagcgtt tttctttact acagccgccg
     2641 gtttgtctgc gtttaactac ctttggtcgt tcggaaagcg atctggcaca aagcctggat
     2701 actctacaac tgccgccggg cgtaacaatg ggctatcgct cctcaatgcc gatcatcgaa
     2761 ctgaaactca ccggaccggc aagcgagcaa caggcgatgg aaatactgtg gctggacgtt
     2821 aaacgtgttg ccggacagag cgtgattttc gaaggcaccg aaggactgcc cgcgcagatc
     2881 agtcgcgaat tgcaaaaccg ccagttcagc ctgacgttga gcgagcaatt caccggcggt
     2941 ttattggctt tgcaactttc tcgcgcaggt gctccattgc tggcgtgtga agtggttcct
     3001 tcacaggaag aaaccctggc gcaaactgcg cactggatta cagaacggcg ggccaaccat
     3061 tttgccgggc tggcactggc tgtttcgggt ttcgagaacg agcatctcaa ctttgcgcta
     3121 gccacgccag acggcacttt cgctctgcgt gtgcgtttca gcactacacg ctacagcctg
     3181 gctatccgtc aggaagtgtg cgcaatgatg gcactgaata tgctgcgccg ttggttaaac
     3241 ggccaggaca tcgccagtga gcatggctgg attgaggttg ttgagtccat gaccttatct
     3301 gtctgattcc gccactctgt gccggtttac tgcaccggca cattccccct ccggcaaaat
     3361 ttgtccaacc tgttgtactc aataagaaaa aatccgtgcg ctgattcaca aatttgtgcg
     3421 taatttctcc agtccgctgg acgaggagcg ggatttgatg cgcttcacaa ttttactccc
     3481 cctgagcaac gagactgatt ccagtatcta agactggagt ccagtatgtt ggaatcaagc
     3541 aaagttcccg ccctgacacg ggctattgat attctcaatt tgattgcccg cattggcccc
     3601 tgtagtgctg cgacgatcat tgacacattg ggaatcccta aaagcacggc ctatttgctg
     3661 cttaatgaac tcaggcgtca gcgttttctt agccttgatc accaggaaaa tttttgtttg
     3721 tgtaccaggc tggtggagct ttctggtcat gcgttaagca agatggatct ccgggaactg
     3781 gctcgaccgc gtttgacgca actgatggat accaccgggc tgttatgtca cctggggatt
     3841 attgataacg gcagcgctta ttacatttta aaagtggagt catcagccac tatcagcgtg
     3901 cgttcccatg aaggaaaaag cctttcgctt tatcgctccg gaataggtaa atgcctgctt
     3961 gcctggcaac ctgcagcggt acagcaaagt attatcgaag ggctggtctg ggagcaggcg
     4021 acacccacaa cgattactca tccacagcaa cttcacgaag aactggcgcg tattcgccgt
     4081 cagggctgga gctatgacaa cggcgaagat taccccgatg tccgttgtgt cgctgcgccg
     4141 gtgtttaacg ccaataacga gcttaccgct gcgatctcgg tggtcggtac ccgcttacaa
     4201 atcaatgaag aataccgtga ttatctggcg ggcaaggcca ttgcctgcgc cagggatatt
     4261 tcacgtctgt tgggatggaa aagtcccttt gacttacaag cctcataaca atggagaaca
     4321 tcatgaccct accaaaaatt aaacaggttc gcgcctggtt cactggcggt gcgacagcag
     4381 aaaaaggcgc tggcggcggt gattatcacg accagggggc gaatcactgg atagacgatc
     4441 atattgccac cccgatgagt aaataccgcg attacgagca gtcacgccag tcatttggca
     4501 ttaacgttct tggcacgttg gttgttgaag tcgaagcaga aaacggccag accggattcg
     4561 ctgtttcgac agccggtgaa atgggctgtt ttatcgtcga aaaacacctt aaccgtttca
     4621 ttgagggtaa atgtgtcagt gatatcaaac tgatccacga tcaaatgctc aatgccaccc
     4681 tgtattactc cggctctggt ggcctggtga tgaatacgat ttcctgtgtc gatctggctc
     4741 tgtgggatct gttcggcaaa gtggtcgggc ttccggttta taaactttta ggcggcgctg
     4801 ttcgtgatga gattcagttc tacgccacag gtgcgcgtcc ggatctggca aaagagatgg
     4861 gctttatcgg tggcaaaatg ccgacgcact gggggccaca tgatggcgat gcggggatcc
     4921 gcaaagatgc cgctatggtc gcggatatgc gtgaaaaatg cggtgaagat ttctggttaa
     4981 tgctcgactg ctggatgagt caggacgtga actatgccac caaactggcc cacgcttgcg
     5041 cgccctataa cctgaaatgg atcgaagagt gcctgccgcc acagcagtat gaaggttatc
     5101 gcgaactgaa acgcaacgcg ccagtcggaa tgatggtcac cagcggtgag caccacggca
     5161 cactgcaatc ttttcgtacg ttttcagaaa ccggtatcga cattatgcag ccggatgttg
     5221 gctggtgcgg tggtttaacc acgctggtgg aaattgccgc aatcgccaaa tccagggggc
     5281 aactggtggt gccgcacggt tcgtctgttt actctcacca tgcggtgatc accttcacca
     5341 atacgccatt cagcgaattc ctgatgacca gcccggattg ttcaacgatg cgtccgcagt
     5401 ttgacccgat tctgctcaat gagccggttc cggtgaatgg tcgtattcat aaatcagtgc
     5461 ttgataaacc cggttttggc gtcgaactca atcgtgactg caatttgaaa cgcccctaca
     5521 gccactaatc actccgggcg ttgctgtgac aacggtaacg cccacatatt ctaaggagct
     5581 ttttatgagc accgctttgc ttgacgccgc ggtgaagaaa aaccgtgtgc gtttaattcc
     5641 gtttatgttg gcgctgtatg tgctggcgtt tctcgaccgt tcgaatatcg gttttgccaa
     5701 acagacctac cagattgata ccgggttgag taatgaagct tatgcgctgg gagcaggcat
     5761 tttctttgtg gtatatgcgt ttctgggtgt tccggcgaat cttttgatgc gcaaactggg
     5821 ggccagaacc tggattggta cgacaacact gctgtgggga tttctttcgg cagccatggc
     5881 atgggccgat actgaagcga aatttctgat agttcgcact ctgcttggtg ctgcggaggc
     5941 cggatttttc cctggtatga tttatctcac ctcgcaatgg tttccgcagc gtaatcgcgc
     6001 cagcattatg gggctgttct atatgggcgc accgctggcg ttaacactgg gatcaccgct
     6061 ttccggcgcg ctgttggaga tgcatggatt tatggggcat cccggctggt tctggatgtt
     6121 tgtgattgaa ggattgttgg cagtcggtgc tggggtattc acattctttt ggcttgatga
     6181 cacaccggag caggcacgtt ttctgagtaa acaagaaaaa acgttgctta tcaatcaact
     6241 ggcaagtgaa gaacaacaga aagtgacttc tcggctgagc gatgcgctgc gtaatggccg
     6301 agtctggcaa ctagcgatta tctacctgac cattcaggtg gcggtttacg gattaatttt
     6361 cttcctgccg acccaggttg cggcattgct gggaacaaaa gtgggcttta cagcgtcggt
     6421 ggtcaccgcc attccgtggg ttgcggcctt gtttgggacc tggcttattc cgcgctattc
     6481 cgataaaacc ggcgaacggc gtaatgtcgc agcgctgaca ttactggcgg cgggcattgg
     6541 tattggtctg tccgggctgc tttctccagt aatggcgatc gtagcgctgt gtgttgcagc
     6601 tatcgggttt attgccgtgc agccggtgtt ctggacgatg ccgacacaac tgctttccgg
     6661 tacggcgctg gctgcgggga ttggttttgt aaacctgttt ggtgcagtgg gcgggtttat
     6721 tgccccgatc ctgcgcgtga aagcagaaac gttatttgcc agcgatgcgg caggattact
     6781 gacactggca gcggtggcgg tcatcggttc gctgattatt ttcactctgc gtgtaaatcg
     6841 tactgttgcg cagaccgacg tggcacatca ttaaataggt taaggaacac gatgaacgca
     6901 ttattaacca atccctttaa agaacgttta cgcaagggcg aagtgcaaat tggtctgtgg
     6961 ttaagctcga ctacttctta tatggcagaa attgccgcta cttccggtta tgactggttg
     7021 ctgattgacg gggagcacgc gccaaacacc attcaggatc tttatcacca gctacaggcg
     7081 gtagcgccct atgccagcca acccgtgatc cgtccggtgg aaggcagtaa atcgctgatt
     7141 aaacaagttc tggatattgg cgcgcaaact ctattgatcc cgatggtcga tactgccgat
     7201 caggctcgtc aggtggcgtc tgccacgcgc tatcctccct acggtgagcg tggtgttggg
     7261 gccagtgtgg cacgggctgc gcgctgggga cgcattgaga attacatggc gcaagttaac
     7321 gattcacttt gtctgttggt gcaggtggaa agtaaaacgg cactggataa cctggacgag
     7381 atcctcgacg tcgaagggat tgatggcgtg tttattggac cagtggatct ttctgcgtcg
     7441 ttgggctacc cggataacgc cgggcacccg gaagtgcagc gaattattga aaccagtatt
     7501 cggcggatcc gtgctgcggg taaagcggct ggttttctgg ctgtggctcc tgatatggcg
     7561 cagcaatgcc tggcgtgggg agcgaacttt gtcgctgttg gcgttgacac gatgctctac
     7621 agcgatgccc tggatcaacg actggcgatg tttaaatcag gcaaaaatgg gccacgcata
     7681 aaaggtagtt attgatatca aaggcccatg gggatcggct gtgggcctgt gttaattagt
     7741 ggttattcgc tgccagatcg gcttcgctta gctgggtggc cgcgagcacc tggtcacgat
     7801 caatgccctg ttccagcatg cgcaaagcaa ttttaatggc ttgttcatgc ataccttcct
     7861 gccagccaat ttgatgccct tcctgccgta gccgttcagc aatagtcatt aatctctcct
     7921 tttgtagtgg tgaacgttcg gcaatctcct caataaaacg ggtgaaacgg gaggtatcgc
     7981 cgcattgcag cagataatta aacagtgttt gtagctggct gtcattagtg aagcctctaa
     8041 ccaaaagcgt ggtgatcctg tcgaccatgc cgattaaatc gcggtcgcga atatgctttt
     8101 gaatcagttc cagcagagct atacgccgat gttgcatgat ctcatcgtca ggtacgatgg
     8161 tgatatccac caacggaaaa gcttcggtgt acaactgccg ggcaagttgc ggctcgtcaa
     8221 actcatccag ccagttaagt gagtacgggt agggcgatgt ttcgccatga taaaacagca
     8281 atggcaccac cagcggaact ctgtcatagc ctttatccag gtgacgctgc atggcggcag
     8341 tggcatagcg cattagccga aaagccatat tcttttctgc agagctttga tgttcaatca
     8401 cgcagtagat atagccgtca ccgtcgctgg tttccacgga ccacaaaaca tccgagtagt
     8461 aagcgcgtaa acttttttca ataaaactag tgggttccag gcgtaaggtt tgcaggttgc
     8521 aaagcttgcg cagtggttct ggtaaatgta tttcgagaaa atcccgtgcg gtttcgggtg
     8581 tgaacataaa ggttttaaac accgcatcat gcggggagga tgttgttgat tcggtcatgg
     8641 ttgtccgtca cctgatattt atgatgacgc gacattacgt ctatcccggg cctttgacat
     8701 ctgtcagttt cagcctttgc gagacatttt cagaggaatt ttcaaagcgt tgcaacgcat
     8761 acatggtgcg tggaatcgcg ctcgcaaaaa tgcccgataa gcaatgtgct taccgggcat
     8821 caaaaggtcg gttaagccag cgcctgggcc agtagcgtaa tcggatgttc gcagcgaagg
     8881 ctcgtggaca tctcaatctg ccatttgcag gtttcgcagt cggtgatgac cagatccgcg
     8941 ccgctctctt ctatctggcg gaacagtggc gcaccgatgg cttgtgaggt ggggtagttc
     9001 tcttttttga aaccgtaagt acccgcaata ccgcagcact gggaatccag caccgttaac
     9061 tcaagccccg ggattttacg caacagctcc agggtgtaga gcgtccagcc cattttttcc
     9121 atatggcacg gagtgtgata aaccactttc agcggcagcg gtttcagcgg taacgttttg
     9181 ccttcgtcca gcttgcgcca cagccagcgg gttgccagtt cgatatgatc gcgcaagcct
     9241 ttgttgtcga cattcagcac ttccgggtat tcgtcgcgca gggcaaatgt acaggttgag
     9301 gaggtggcaa tcaccggaat gccttttact cccacagctt cgcggatcga ctctacattc
     9361 gtaattgcct gtttgcgtgc tttatcggta aagccgttgg cgattagcgg tacgccgcag
     9421 catttttctt tgctgagcag ttgtacaccg gtacccattg cgttgagcac tttaattaaa
     9481 tctttaccta actgcggatg gttgtagtta acgaagcagc cgtgaaagaa agcgacctgg
     9541 tctttatatt gtgcttgctg agccgctatg ctgcgatacc agcgacggaa cgtgccgaag
     9601 gagtatttcg gtagcgtgcg gcgatgatcg atttttaacg ccgcatcaag cagctgccgc
     9661 accggtttca gcgaggtagc ggtgttgacg attggtgcga acggcgtgga aacgctaccc
     9721 atcaggtcgg tatgactcaa cacaaaatta cgcagcgacg ggcgcgtggt gtcatatttt
     9781 gcccgcgcgc gctggataat atcgccaatc ttcacatcgg acgggcaggc gacttcacaa
     9841 cgtttgcagt tgatgcaata tttcagcgcc tcgtcataca gtgcgccatc tttcaaacgc
     9901 agacgctcgc catccggccc ggcttgtttt ggccctggat aaccgggatt cacccggctc
     9961 accgggcagg cggtggtgca gacggtgcac ttaatgcagt tttcgaagct ggtgtcgttc
    10021 attgttggcc tcctgcgcgt tgggcaatct gttgagcggc atgtaaagca ctgacggcac
    10081 aaacaccgcc gccgcatccc tgggcgatgg gatcaaatcc gcccagcacc gaaccgatgg
    10141 caaacaggtt ctcaatggtt tgccctgcct gcgacgggcg tagcgtctca tcagtggtta
    10201 caccgaactg ctgccacggt tgcggcgcaa aaaaatcgcc cttataccat tcaccccgcg
    10261 tggcggtttg tagcacatca aggccgagaa tcggctcgcg aatgccgtta cgttccgcta
    10321 ccagtccgcc actaaagaaa ctgccgctgg cgagaaccgc gaaacgtgga cgtagcggaa
    10381 tatcggcgtg attgcgggtc cagatttcat tcactacgcc atttttacag gtcacttttt
    10441 tcacttcatc gcccggcatc cacacgccac cctggcgcac aaactggcgc tgtaactggt
    10501 tttgcagacg aatgcccagc acggaaggcg gcagcgttgg caaaagcatc agtgaacaag
    10561 gtagtttttc attcaaccaa cgccacagtt tgtcatcggc caggccgaag caggcgggca
    10621 tcaggatcat ttcgcaggta ttggcgacag gaataagcgc atcaagtaac agcggccagt
    10681 tttcttcatt atcaaggaaa cgggcgatat tcacagcgcg aaattcggtg gcgttattgc
    10741 gcaggacatc cagttccggc agctctattt ctgcggtttc aaccgaaaga tccagttcgc
    10801 gcaacgaagc cgccgcaaga tgcgcctgaa aatccatcag gccgcttatc ccgaccacac
    10861 atattttctt cgcgggcaac ggccagacgg ggacttctgg cgaacttagc caggtagagc
    10921 gcagagtgcc gagcggcgta atccgctggt gagccagttc tacgctgccc tgcaattgcg
    10981 caccgctttc agcgattaat gcctgcgcct ggcaagcgag atcgagcacg cgttgtggcc
    11041 cgagaagaga gtaaggatgt gcaggtgcct gctgacgcaa agattccagt ccactgtgaa
    11101 tgtctgccac gggttgacca tctggcagat ggctcagcaa atccagcgaa ccggatgaga
    11161 aatgcaacgc gctttgacca cgcgtgacaa tggcacagcg caggccgtgt ttttgcagtt
    11221 gcaggccaca gagtaatccg gcgaggccgc cgcccataat gacagtatca aagcgcatct
    11281 ttctgctcct tctccagacc acacagtccc tgataaaccc agcgggtaaa ttcgctttcg
    11341 cgcagtgcgt ctccccaggc gatgggttgc acgcctttcc agcgttcgtt aaggaaggtg
    11401 gaaagttgct cgatagattg cgcggacgtc gtgacgttaa accgttgcag cagtccggca
    11461 gcgcggcagg cgcagagttc gccctggcag gtgcccatcc ccacacgggt acgacggcgt
    11521 aaatccagca ggctattaac gtttaaattt tctaccgcgt actgaacttc acccgcagtt
    11581 accgcttcgc actcacatac caggctacga tgcagacggc cttcgctcag ccaggcaggc
    11641 gtgcgatcgc catgacgata aaccgcagaa ccgcgcagcg gggcaggcag ggagatgact
    11701 ttacgcaagg taacttcagc gggttcttgt gaaccaggca gtgccagatc ggcagtcgta
    11761 caggggcgcg tgttgcccag tttgcggcat accgcgtcgg tagcccattc agccatcagc
    11821 cgataggtca tcagtttgcc accggtgatg gtgataaatc cgtccagacc atcgcgttca
    11881 gcatggtcga gcagcacgat gccacggctg acgttacgtc cgctcgggtc gtcatcgctg
    11941 gcaaccagcg ggcgcacgcc agaataggcc cgcaaaatgc gcgttttcgc catcacgggg
    12001 gccagttttt ccccttcacg cagcagaata tcaacctctt ctgccgtcac tcgattatcg
    12061 tcaatctcgt tgtaatcaat acgtaaagag gtggtgccaa tcagcgaaat ggtatcgcca
    12121 ggcaccagaa tatcggcgtc ggaaggttta cggcagcggt tgatcacatg ctggttaatg
    12181 cggtgatcca tgatcagcag cgatcctttc gccgggaaca tgcgaatgcg cagatcggca
    12241 tattcggcaa tgtgttgccc ccagatccca gcggcattaa ccacgacagg tgcatgaagg
    12301 gcctgagttt cgccggtgag atggttacgt acacgaacac cgcacaccgt cgcgccttca
    12361 cgaatcagcc ccgtgacttc atgagcggta aggataacgg caccgtgttc tttggcatcc
    12421 agcatgtttg ctgcggtcag acgaaatgga tcaacggtgc catccggaac tttcaccgcg
    12481 ccaatcagtg ccgggttaac ggcaggttcg ataatgcgcg cttgctgcgg gtctatagct
    12541 tctgcgctga tccctgcttc ttcgcaggcg cgaataaaag tggcctggaa ggataggtca
    12601 tcttccggca gggtgataaa caggccgttg gttggttcaa cgcagtgacg tgcaatgcgt
    12661 ttcaggatct ggttttcact aatgcattcg cgggccgatt ccgcatcggt taccgcatag
    12721 cgcgcaccgc tgtgcagcag gccgtggtta cgcccggtgg caccggttgc gatgtcgtgg
    12781 cgctcaacca aaatcacgcg cagcccgcgc agggcacagt cgcgggcaat cccggctccc
    12841 gttgcgccgc cgccaatgat aatcacgtca cttgattgcg agtcgcgagt tttcattgtt
    12901 atccctctga agttcgtttt ttaccattta gccatagtaa aaacatgaat tgtttgattt
    12961 cgcgcatatt cgctcataat tcgaaagtta aatgtgattt catgcgtcat tttgaacatt
    13021 ttgtaaatct tatttaataa tgtgtgcggc aattcacatt taatttatga atgttttctt
    13081 aacatcgcgg caactcaaga aacggcaggt tctctcactg aatcaggctg ttaatcataa
    13141 ataagaccac gggccacgga ggctatcaat gttgagtatt tttaaaccag cgccacacaa
    13201 agcgcgctta cctgccgcgg agatcgatcc gacttatcgt cgattgcgct ggcaaatttt
    13261 cctggggata ttctttggct atgcggctta ctatttggtt cgtaagaact ttgcgcttgc
    13321 tatgccttat ctggttgagc agggattctc acgcggtgat ttaggttttg ccctttcggg
    13381 gatctcgatt gcttatggat tttcgaaatt catcatgggt tcggtatcgg atcgctcgaa
    13441 tccgcgcgtt ttcctgcccg caggtttgat tctggcggcg gcagtgatgt tgtttatggg
    13501 ctttgtgcca tgggcgacgt cgagcattgc ggtgatgttt gtactgttgt tcctctgcgg
    13561 ttggttccag gggatggggt ggccgccgtg tggtcgtact atggtgcact ggtggtcgca
    13621 gaaagaacgt ggcggcattg tgtcagtgtg gaactgtgcg cacaacgtcg gtggtggtat
    13681 tccgccgctg ctgttcctgc tggggatggc ctggttcaat gactggcatg cggcgctcta
    13741 tatgcctgct ttctgcgcca ttctggtggc attattcgcc tttgcgatga tgcgcgatac
    13801 cccgcaatcc tgtggcttgc cgccgatcga agagtacaaa aatgattatc cggacgacta
    13861 taacgaaaaa gcggaacagg agctgaccgc gaagcaaatc ttcatgcagt acgtgctgcc
    13921 gaacaaactg ctgtggtaca tcgccatcgc caacgtgttc gtttatctgc tgcgttacgg
    13981 catcctcgac tggtcaccga cttatctgaa agaggttaag catttcgcgc tggataaatc
    14041 ctcctgggcc tacttcttct atgagtatgc gggtattccg ggcaccctgc tgtgcggctg
    14101 gatgtcggac aaagtcttcc gtggtaaccg tggggcaacc ggcgtcttct ttatgacact
    14161 ggtgaccatc gcgactatcg tttactggat gaacccggca ggtaacccaa ccgtcgatat
    14221 gatttgtatg attgttatcg gtttcctgat ctacggtcct gtgatgctga tcggtctgca
    14281 tgcgctggaa ctggcaccga aaaaagcggc aggtacggca gcgggcttta ccgggctgtt
    14341 tggttacctg ggcggttcgg tggcggcgag cgcgattgtt ggctacaccg tggacttctt
    14401 cggctgggat ggcggcttta tggtaatgat tggcggcagc attctggcgg ttatcttgtt
    14461 gattgttgtg atgattggcg aaaaacgtcg ccatgaacaa ttactgcaaa aacgcaacgg
    14521 aggctaatgg catgaaattg acgctgaaaa accttagcat ggcgatcatg atgagcacta
    14581 tagtcatggg aagcagtgca atggcggcgg acagcaacga aaaaatagtc atcgcccatc
    14641 gcggtgccag tggatatttg ccggagcata cgctgccagc aaaagcgatg gcgtatgcgc
    14701 agggagcgga ttatctggaa caggatttgg tgatgaccaa agacgaccat ctggttgttc
    14761 tgcatgacca ttacctcgat cgtgttactg atgttgccga tcgtttcccg gatcgggcgc
    14821 gcaaagacgg tcgttactac gcgatagatt tcacgctgga tgaaattaag tcgttgaaat
    14881 ttaccgaagg tttcgatatt gaaaacggta aaaaagtgca gacttatccg gggcgtttcc
    14941 caatgggtaa gtccgacttc cgggtgcaca cctttgaaga agagattgaa tttgttcagg
    15001 ggttaaatca ctctaccggg aaaaatatcg gtatttatcc agaaatcaaa gcgccgtggt
    15061 tccatcatca ggaagggaag gatattgcgg caaaaacgct ggaagtgctg aagaaatatg
    15121 gttacaccgg taaagacgat aaagtttatt tgcaatgttt tgatgctgat gagctgaagc
    15181 gtattaagaa tgagctggaa cccaaaatgg gcatggagct caatctggta cagttgattg
    15241 cctataccga ctggaatgaa acgcagcaga aacagccgga tggaagctgg gttaattaca
    15301 actacgactg gatgtttaag ccgggtgcta tgaaacaggt ggcggaatat gccgacggta
    15361 tcgggccgga ttaccatatg ttgattgagg aaacatcgca gccgggtaat atcaaactca
    15421 ctggcatggt gcaagatgct cagcagaata aactggtagt gcatccttat accgtgcggt
    15481 cagataaact gcctgaatac actactgatg tgaatcagtt atatgatgct ctgtataaca
    15541 aagcgggtgt aaatgggctg tttactgatt tccctgataa ggcagtaaaa ttccttaata
    15601 aagagtaatg ctactgcagg atggcgttca tgcgccatcc tgtctcacat caaatttttc
    15661 cactcaccac gagttgttcc gtacacgttt gtagtaaaaa cagtggactg ccttctaccg
    15721 gcttttcttt agccaacaac gaaatctgtc gatagaaggt tggttcgtga ggaacggaac
    15781 acaattcacc tgcaatgctt ttcagcgtta actctggttg taaggcgatg cctaatccct
    15841 gtcgaataaa attcatcgct gtatccgact gattaaattc acattttatc tgctgggcgc
    15901 actattagtg gtagtggctg gtggaatcta tgcgctgcaa atgaagggca aaagcggtgt
    15961 cacacctgta gcggcaaaag aaattcatta atacgacacg tttcattaag attttcctca
    16021 ggtaaccacc tatagtcatt gtcgttaata aaaatgacag gcgatagggt atggcagttt
    16081 cagcaaagta cgacgaattt aatcactggt gggcaacaga gggcgactgg gttgaagagc
    16141 ctaactatcg ccgtaatggt atgagcgggg tgcaatgcgt cgagcgcaac ggcaaaaagc
    16201 tgtatgtaaa gcgcatgacg catcatctgt ttcattccgt acgttatccg ttcggccgac
    16261 caacgattgt ccgtgaggtt gcggtaatta aagaactgga acgggccggt gtcatcgtgc
    16321 cgaagatcgt ttttggtgaa gcggtgaaaa ttgagggtga atggcgagcg ttgctggtga
    16381 ctgaagatat ggcggggttc atcagcattg ctgactggta tgcccagcat gcagtatcgc
    16441 cttattctga cgaagtacgg caagccatgt tgaaagcagt ggcgctggcg tttaagaaaa
    16501 tgcatagcat taatcgtcag catggctgtt gttatgttcg ccatatctat gtgaaaacag
    16561 aaggtaaggc agaagctggt tttcttgacc tggagaaaag tcgtcgccgc ttgcgtcgcg
    16621 ataaagcgat taatcatgac ttccgccagc tggaaaaata tctggagcca atccccaaag
    16681 cggactggga gcaggtgaaa gcgtattact acgccatgta atcgttgtag gccagataag
    16741 acgcataagc gttgtatctg gcactacaac caattcacat ctcgatttca atatcgcctt
    16801 ttgcccggca acaacagggc aaaatttccc ccggctgaat aaaggctaac ggttcggcaa
    16861 tccagtcaac ctgacctgca accagacggg tgcgacagga gccgcagtaa ccttcgcgac
    16921 actgatactc aaccgccaca ttgtgggatt ccagcgccgc cagaagggaa gggtgttcat
    16981 cctggcacag cagttgtgtg ccagtgatgc gcagggtaac gcgggccatc agagttggaa
    17041 gttactcaaa tcgtcggtgt ccacttccgc gtcaatctgc ccgaccagat aagaactgac
    17101 ttccacttcc tgcggagcaa cctgcacgtt atcagacacc agccaggtgt tgatccatgg
    17161 gatcgggttg gagcgcgtct ggaacggcag atccaaaccg actgcctgca tacggatatt
    17221 ggtgatgtat tcaacgtact ggcagagaat gtctttattc agaccaatca tcgaaccgtc
    17281 gcggaacaga taatccgccc agtctttctc ctgttgagct gcctgaacaa acaggtcata
    17341 gcactcctgc ttacactctt cggcaatttc cgccatctca ggatcgtccg cgccgctgcg
    17401 cagcagattc agcatatgct gggtgccggt caggtgcagg gcttcgtcgc gggcaatcag
    17461 gcgaataatt ttggcgttgc cttccatcaa tttgcgttct gcaaatgcga aggaacaagc
    17521 aaagctgacg tagaaacgaa tcgcttccag cgcgttaacg ctcatcaggc agagatacag
    17581 ttttttcttc agctcgcgca ggctaacggt cacagtttta ccgttaacgg tgtgggtacc
    17641 ttcgcccagc agatgccagt agcaggtcat ttcgatcagc tcatcgtaat agctggagat
    17701 cccttccgca cgtttctgga tctgctcgtt ggtgacgata tcgtcaaaca caacagacgg
    17761 atcgttaacg atattacgaa tgatatgagt ataggaacgg gaatgaatcg tttctgaaaa
    17821 cgcccaggtt tcgacccagg tctccagttc cgggatcgag atcagcggca gcagtgccac
    17881 gttcgggcta cggccctgaa tggaatccag cagtgtctga tatttcaggt tgctgataaa
    17941 gatgtgtttt tcgtgttcag gcagtgcctg gtaatcgata cggtcgcgag aaacgtcaac
    18001 ttcttccgga cgccagaaga aggagagctg cttttcgatc agcttttcga agatgtcata
    18061 tttttgctga tcgtagcgag ccacgttgac cggctgacca aagaacatcg gttctttgag
    18121 ctgatcattt ttcgtctgtg aaaaggtggt atatgccatg agtgtgtcct gttgggagtt
    18181 taatgccgga taaggcattt tacgccgcat cctgtgcctg atgcgacgct ggcgcgtctt
    18241 atcaggccta caaatccgag ccgtaggccg gataaggcgt tcacgctgca tccggccccc
    18301 ggagcctgat gcgacgctgg cgcgtcttat caggcctaca aaccgagccg tagaccggat
    18361 aaggcgttta cgccgcatcc ggcatctcaa tatcagatct tacatgcgcc gctttcgcag
    18421 ccatcgtcct ggattgacgg caccagatcg tcctgcgcgt cttcagcgcc gtcacgggtg
    18481 ttctgataat acagcgtttt gaccccgaat ttgtaggcgg tgagcaggtc tttcagcaac
    18541 tgctgcatcg gcacttttcc tgacgggaag cgtgacggat cgtagttggt gttagcagag
    18601 atcgactgat cgataaattt ctgcatgata cccaccagtt gcaggtagcc atcgttaccc
    18661 ggcatttccc acagcagctc ataggcgtcg tgcagatgct cgtagtccgg caccacctgg
    18721 cgcagaatac cgtctttcga cgctttgatg ctgacgtaac cgcgcggcgg ttcaataccg
    18781 ttagtggcgt tagagatctg cgaagaagtc tcggacggca tcagggcaga aagcgtggag
    18841 ttacgcaggc cgtgcgtttt gattgactca cgcagagctt cccagtcgta atgcagcggc
    18901 tcattagcga tggtatccag atctttctta taggtatcga tcggcaggat ccctttcgcg
    18961 taagtggttt cgttaaacca cgggcacgcg ccttgctctt ttgccagctc gttagacgct
    19021 ttcagcaggt aatactgaat cgcttcgaag gttttatgcg tcaggttgtt ggcgctgccg
    19081 tcggagtaac gcacaccgtg cttcgccagg tagtaagcga agttgatcac accaataccc
    19141 agcgtacgac gacccatcgc tccacgtttg gcggccggga tcgggtaatc ctgataatcc
    19201 agcagcgcgt caagtgcacg aaccgccaga attgccagct cttccagttc atccaggcta
    19261 ttaattgcgc ccaggttgaa agcagacagc gtacacagcg cgatttcacc gttctcgtcg
    19321 ttgacgtcgt tcagcggttt ggtcggcagg gctatctcca ggcacaggtt agactgacgc
    19381 actggcgcga tggccggatc aaacgggcta tgggtattgc agtggtcaac gttctgaata
    19441 tagatacgac cggtagacgc acgttcctgc atcatcagcg agaacagctc aacggctttc
    19501 acacgctgct tgcggatgct gtcgtctttc tcatatttgg tatacaggcg ttcaaactct
    19561 tcctgatcgg cgaagaatgc gtcgtacagc cccggtacgt cggacgggct gaacagggtg
    19621 atatcttcac ctttcagcag gcgggtatac atcagtttgt tgatctgtac cccgtagtcc
    19681 atatggcgca cgcggttgcc ttccacacca cggttgtttt tcaacaccag caggctttcc
    19741 acttccagat gccacatcgg gtagaacagc gttgccgcac cgccgcgcac gccgccctga
    19801 gagcaggatt tcaccgctgt ctggaaatgt ttgtagaacg gaatacagcc ggtatggaac
    19861 gcttcaccac cgcgaatcgg gctacccagc gcacgaatac gcccggcgtt gatgccgatc
    19921 ccggcacgct gggaaacgta tttcacaatt gcgctggagg tagcgttgat ggaatccaga
    19981 ctgtcaccgc actcgatcag tacgcaggag ctgaactgac gagtcggggt acgcacgccg
    20041 gacatgattg gcgtcggcag cgaaatttta aatgtggaaa ccgcgtcgta aaaacgcttc
    20101 acatattgca ggcgcgtttc acgtgggtag ttcgagaaca ggcacgcggc aaccagaata
    20161 taaaggaact gggcgctttc gtagatttcg ccggtcacac gattctgtac cagatatttg
    20221 ccttccagct gcttaacggc agcataagag aaggtcatat cacggtcgtg atcgataaac
    20281 gtgtccatct gcttgaactc ttcttccgtg tagtcttcca gcagatgatt atcgtatttg
    20341 cccatctcga ccattttcac cacatggtcg tacagcgcag gcggctcaaa ctggccgtag
    20401 gcttttttac gcaggtggaa gatcgccagg cgcgcggcga gatgctgata atccggcgca
    20461 tcacgggaga tcaggtctgc ggcagccttg ataatggttt cgtggatgtc agaggtcttg
    20521 ataccgtcat aaaactgaat gtgggagcgc agctcgactt gggaaatcga aacgttatgc
    20581 agtccttctg ccgcccaatc cagaacgcga tggattttgt cgagattgat gcgctctgtg
    20641 ctaccgtcgc gctttgtcac cagcagattc tgattcatgt atgtcgtacc tgtttttgga
    20701 attctttccg caatagtttc atgcagacta cctgtagtac gggaagctgc ttagaacact
    20761 atatataggg ggtatgtttg ggattcactg caagatagtg tgaaaatggc cctcttgcaa
    20821 gtgcataact ttgtggataa ctcaggaagg aaaaagtggc tttcgcgcac cttaggtcag
    20881 acaaggtgtc cgggaaagtc aatgggaaga gaaaaatttg ttaaaaataa ctgttcggcg
    20941 atttcttgaa cggcagaata ggttggataa attgatatag atggtttatt aatctttttg
    21001 ctaatgattg caaaaattga tctaagcaca agtcatcaca taatctgatg tatcagttgt
    21061 ggtcaatata taagcgaatg taagcgattg ttgatgtcga aatggttgtt ttctattcgg
    21121 ttattgttaa atgcgaagcc attttgttaa tttaccttat attataagaa aattattcat
    21181 tactttaatt tagtttaaat ataactggta tcccttaagg ttattaactt gaattatgac
    21241 gcactgatat tattcatcaa ata
//
LOCUS       DAZKHC020000073        20906 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZKHC020000073 DAZKHC020000000
VERSION     DAZKHC020000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.073"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(228..1877)
                     /gene="actP"
                     /locus_tag="ACHOSM_RS16345"
                     /old_locus_tag="pgaptmp_003269"
     CDS             complement(228..1877)
                     /gene="actP"
                     /locus_tag="ACHOSM_RS16345"
                     /old_locus_tag="pgaptmp_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HGU4559730.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1874..2188)
                     /gene="yjcH"
                     /locus_tag="ACHOSM_RS16350"
                     /old_locus_tag="pgaptmp_003270"
     CDS             complement(1874..2188)
                     /gene="yjcH"
                     /locus_tag="ACHOSM_RS16350"
                     /old_locus_tag="pgaptmp_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HGU4559731.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2388..4346)
                     /gene="acs"
                     /locus_tag="ACHOSM_RS16355"
                     /old_locus_tag="pgaptmp_003271"
     CDS             complement(2388..4346)
                     /gene="acs"
                     /locus_tag="ACHOSM_RS16355"
                     /old_locus_tag="pgaptmp_003271"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HGU4559732.1"
                     /translation="MSQIHKHTIPANIADRCLINPEQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4739..6175
                     /gene="nrfA"
                     /locus_tag="ACHOSM_RS16360"
                     /old_locus_tag="pgaptmp_003272"
     CDS             4739..6175
                     /gene="nrfA"
                     /locus_tag="ACHOSM_RS16360"
                     /old_locus_tag="pgaptmp_003272"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HGU4559733.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCADCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6220..6786
                     /gene="nrfB"
                     /locus_tag="ACHOSM_RS16365"
                     /old_locus_tag="pgaptmp_003273"
                     /pseudo
     CDS             6220..6786
                     /gene="nrfB"
                     /locus_tag="ACHOSM_RS16365"
                     /old_locus_tag="pgaptmp_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
     gene            6783..7451
                     /gene="nrfC"
                     /locus_tag="ACHOSM_RS16370"
                     /old_locus_tag="pgaptmp_003274"
     CDS             6783..7451
                     /gene="nrfC"
                     /locus_tag="ACHOSM_RS16370"
                     /old_locus_tag="pgaptmp_003274"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HGU4559734.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVS"
     gene            7451..8407
                     /gene="nrfD"
                     /locus_tag="ACHOSM_RS16375"
                     /old_locus_tag="pgaptmp_003275"
     CDS             7451..8407
                     /gene="nrfD"
                     /locus_tag="ACHOSM_RS16375"
                     /old_locus_tag="pgaptmp_003275"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HGU4559735.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLMLWLAKIFGHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAVLGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8427..10145
                     /gene="nrfE"
                     /locus_tag="ACHOSM_RS16380"
                     /old_locus_tag="pgaptmp_003276"
     CDS             8427..10145
                     /gene="nrfE"
                     /locus_tag="ACHOSM_RS16380"
                     /old_locus_tag="pgaptmp_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HGU4559736.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10138..10521
                     /gene="nrfF"
                     /locus_tag="ACHOSM_RS16385"
                     /old_locus_tag="pgaptmp_003277"
     CDS             10138..10521
                     /gene="nrfF"
                     /locus_tag="ACHOSM_RS16385"
                     /old_locus_tag="pgaptmp_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HGU4559737.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASHLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10518..11114
                     /gene="nrfG"
                     /locus_tag="ACHOSM_RS16390"
                     /old_locus_tag="pgaptmp_003278"
     CDS             10518..11114
                     /gene="nrfG"
                     /locus_tag="ACHOSM_RS16390"
                     /old_locus_tag="pgaptmp_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HGU4559738.1"
                     /translation="MKQPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASKQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11456..12769
                     /gene="gltP"
                     /locus_tag="ACHOSM_RS16395"
                     /old_locus_tag="pgaptmp_003279"
     CDS             11456..12769
                     /gene="gltP"
                     /locus_tag="ACHOSM_RS16395"
                     /old_locus_tag="pgaptmp_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HGU4559739.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12847..13536)
                     /gene="yjcO"
                     /locus_tag="ACHOSM_RS16400"
                     /old_locus_tag="pgaptmp_003280"
     CDS             complement(12847..13536)
                     /gene="yjcO"
                     /locus_tag="ACHOSM_RS16400"
                     /old_locus_tag="pgaptmp_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HGU4559740.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13630..15777)
                     /gene="fdhF"
                     /locus_tag="ACHOSM_RS16405"
                     /old_locus_tag="pgaptmp_003281"
     CDS             complement(13630..15777)
                     /gene="fdhF"
                     /locus_tag="ACHOSM_RS16405"
                     /old_locus_tag="pgaptmp_003281"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15358..15360),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HGU4559741.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLSGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15975..17441)
                     /gene="mdtP"
                     /locus_tag="ACHOSM_RS16410"
                     /old_locus_tag="pgaptmp_003282"
     CDS             complement(15975..17441)
                     /gene="mdtP"
                     /locus_tag="ACHOSM_RS16410"
                     /old_locus_tag="pgaptmp_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HGU4559742.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHRASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLKRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17438..19489)
                     /gene="mdtO"
                     /locus_tag="ACHOSM_RS16415"
                     /old_locus_tag="pgaptmp_003283"
     CDS             complement(17438..19489)
                     /gene="mdtO"
                     /locus_tag="ACHOSM_RS16415"
                     /old_locus_tag="pgaptmp_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HGU4559743.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLVIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19489..20520)
                     /gene="mdtN"
                     /locus_tag="ACHOSM_RS16420"
                     /old_locus_tag="pgaptmp_003284"
     CDS             complement(19489..20520)
                     /gene="mdtN"
                     /locus_tag="ACHOSM_RS16420"
                     /old_locus_tag="pgaptmp_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HGU4559744.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIIELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRYWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20539..20814)
                     /gene="ytcA"
                     /locus_tag="ACHOSM_RS16425"
                     /old_locus_tag="pgaptmp_003285"
     CDS             complement(20539..20814)
                     /gene="ytcA"
                     /locus_tag="ACHOSM_RS16425"
                     /old_locus_tag="pgaptmp_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HGU4559745.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4716 a         5536 c         5719 g         4935 t
ORIGIN      
        1 ttaatctggc aaagagagaa aaacatatta tttaaaccat tatagcgcat aaaatatcct
       61 tataattaac aagaaagaaa aggcatattc tctgcattat cattttctac gactgtcaaa
      121 aatcgctcat tttttaatga gtttatttgt ttaatatttt gggaaaaggt gatgcatgct
      181 ggagaggaag agtattcccc ggtcaggcga ccggggaagg ggagagatta atgtgcgcgg
      241 ccttgctcaa cgccaaagcc ggtctgggag cggataaact gcgcgcggaa cagttcacgc
      301 tcgcgcgccc cttccgctga gttatcggtt gccgagaaga accagatgcc gaggaatgcc
      361 acggtgatag agaacagcgc cgggtattca tacgggaaga tggctttttc gtgaccaagg
      421 atctgtaccc aaatcgtcgg gccgaggatc atcagtacta ctgcggtaat cagccccagc
      481 cagccaccca tcatcgcgcc acgcgtggtc agtttcgacc agtacataga aagcagaatg
      541 atcgggaagt tacagctcgc cgcgatggca aacgccagcc ccaccataaa ggcgatgttc
      601 tgattctcaa acagcacgcc gaggataatc gcaatcacgc cgaggatcag tacggtgatt
      661 tttgataccc gcagctcttc acgttcggtc gcgccttttt tgaagacgtt agcgtacaag
      721 tcatgcgaaa ccgcggatgc gcccgccagc gtcagacccg caaccaccgc gaggatagtg
      781 gcgaaagcaa ccgctgaaat aaaaccgagg aacaggttgc cgcccactgc attcgccagg
      841 tgaacggccg ccatgttgtt accaccaatc agatggcccg ccgcgtcttt atattccgga
      901 ttcgcaccaa ccagcatgat cgcgccgaag ccgataataa aggtcagaat atagaagtag
      961 cccataaacc cggtggcgta gaacacgctc ttacgtgctt cgcgggcatc gctgactgta
     1021 aagaagcgca tcagaatgtg cggcaagccc gccgtaccaa acatcagtcc cagacccaga
     1081 gagagcgcgg agatcggatc tttcaccagc ccgcccggct tcatgatgtc gacacctttc
     1141 gggtgtaccg ccatcgcttc actgaacaga ttgttgaagc taaagccgac gtgtttcatc
     1201 accataaagg ccataaagct ggcaccgaac agcaacagca cggctttgat aatttgcacc
     1261 caggtggtcg ccagcatgcc gccgaacagg acgtacatca tcatcagcac gccgaccagc
     1321 accaccgcaa tgtgatagtt aaggccaaac agcagctcga tcagtttacc tgcgcctacc
     1381 atctgggcga taaggtaaag cgccaccacc accagagaac cacaggccga aagaatacga
     1441 atcggccctt gtttcaggcg gtaagaggct acatcggcaa aggtgtagcg tcccaggtta
     1501 cgcagacgtt cggcaatcag gaacaaaatg atcggccagc ccaccaggaa gcccagcgag
     1561 taaattaagc cgtcatagcc ggaggtaaac accagcgcgg agatccccaa gaatgaggcg
     1621 gcggacatat agtccccggc aatcgccagc ccgttctgga agccagtgat attgccgcct
     1681 gccgtgtagt agtcgctacg agaacgtacg cgttttgatg cccagtaggt aatgccgagc
     1741 gtaaacacga cgaaaatcag gaacataata atcgcctgcc agttcgttgg ctggcgctct
     1801 acggccccgc taatagcatc cgcggcgtta gctgcgaaag ggagtgtggc ggcaagcgcc
     1861 gtcagaactc ttttcatgat gcttgtacct catgcaggac ttcattatta agacggtcga
     1921 attcgccgtt cgcccgccag atgtagatac cggtgagaac aaaggagatc acaatcactc
     1981 caacaccaat tggaataccg cgtgtgacgc tggtgttcgg attcagcggc gtgcccagcc
     2041 agccgggcgc gaaggcgatc agtaaaataa agccgatata aactgccagc ataataatcg
     2101 acaggatggt ggcaaaccgt tgccgttttt cgactaactc cctgaaatgc gcattgtctt
     2161 ctatccgctg ataaatagtg ccattcatca cagattctcc agaggtaatg tagggattgt
     2221 tttaattccc gctcccttat gggagaaggt taacgctcgg gtaacccttg ccgaatgtag
     2281 gccggataag gcgtttacgc cgcatccggc aatcaatgcc tgatgcgacg ctgtcgcgtc
     2341 ttatcaggcc tacaaaccgt taccgactcg catccggcaa ttgtgggtta cgatggcatc
     2401 gcgatagcct gcttctcttc aagcagcttc tcgactacgc caggatcggc aagcgtcgag
     2461 gtatcgccca ggttgctggt atcgcccgcc gcaattttgc gcagaatacg gcgcataatt
     2521 ttgccggagc gggttttagg cagggagtcg gtccagtgca gcacgtctgg cgtcgccagc
     2581 gggccaatct ctttacgcac ccagttgcgg acttctgcgt acagttctgg tgacggttcc
     2641 tccccgtgat taagcgtgac gtaggcgtag atcgcctgac ctttaatatt gtgcggaata
     2701 cctactacgg cggcttcggc aatcttcgga tgcgccacca gcgccgactc aatctctgcc
     2761 gtccccagac ggtgaccgga gacgttcagc acgtcgtcca cacgcccggt gatccagtaa
     2821 tagccatctt catcgcgacg cgcgccgtcg ccgctgaaat acatattttt gaaggtggag
     2881 aagtaggtct gttcaaaacg ttcgtgatcg ccaaacagcg tacgcgcctg acccggccag
     2941 gagtcggtga tcaccaggct gccttcggta gcgccttcca gcgggttacc ttcgttatcg
     3001 accagcgccg gttgcacgcc gaagaacgga cgtgttgccg aaccggcttt cagctcggta
     3061 gcgccaggca gcggcgtgat catgaaaccg ccagtttcgg tctgccacca ggtatcgacc
     3121 accggacatt tctcgttgcc gatttttttc cagtaccact cccacgcttc cgggttaatt
     3181 ggctcgccca cggaaccgag aatgcgcagc gacgaacggt cggtaccctc gatcgcttta
     3241 tcgccttccg ccatcagcgc gcggatcgcc gtgggtgcgg tatagagaat attgacctga
     3301 tgcttgtcca ccacctgcgc catacgggca ggcgtcggcc agttgggtac gccttcaaac
     3361 atcagcgtgg tcgcaccgca ggccagcggg ccgtacagca agtaactgtg tccggtcacc
     3421 cagcccacat cggcggtgca ccagtagata tcacccggat gataatcaaa gacatattta
     3481 aaggtcagcg ccgcgtacac cagataaccg ccggtagtat gcagcacacc ttttggctta
     3541 ccggtagaac cggaggtgta gagaataaac agcggatctt cggcgttcat ctcttccgcc
     3601 tggtgctgat cgctcgcttg ctcaaccagg tcgtgccacc acaggtcgcg cccttcctgc
     3661 cagtcaattt tcccgccagt acgcttcagt accaccacat gctctacgct ggtgacgttc
     3721 gggtttttca gcgcgtcatc aacgtttttc ttcagcggaa tactgcgccc ggcacgcaca
     3781 ccttcgtcgg aagtgatcac cagtcgtgag ttggaatcaa taatgcgccc ggcaacggct
     3841 tccggcgaga agccgccgaa aatcaccgaa tgcaccgcgc caatgcgggc gcaggccagc
     3901 atcgcaaccg cggcttccgg caccatcggc atataaatcg ccaccacatc acctttttta
     3961 atgcccagct cgagcagggt attggcgaag cggcagacgt cgcggtgcag ctctttatag
     4021 ctgatatgtt tgctctggct ggcgtcgtcg ccttcccaga tgatggcggt acgatcgccg
     4081 ttttcttgca gatggcggtc aaggcagttt gccgccagat tcagcgtgcc gtcctcgtac
     4141 catttaatgg acacattacc gggggcaaag gaggtgtttt tcaccttctg gtaaggtttg
     4201 atccagtcaa gaatttttcc ctgttcgccc cagaaggtat caggtacgtt aatagattgt
     4261 tgatacatcg cctcgtactg ctcagggttt atcaggcaac ggtctgcgat gttggcagga
     4321 atggtgtgtt tgtgaatttg gctcatgctt ttgttctcct tgtaggatgt taataatatg
     4381 tggcataagc gttaaatgta ggggtattgg cagttttgtt tagtatttgg gcgacagatc
     4441 acgcaaaagt agaattgtgc aaataaacgg cagggtaatt tttgaaggtc agcaacaaaa
     4501 gttgattaat tctttgagga acatgcagtt atgcatgctg ttgaaaaaga ggaagatact
     4561 gactaactct aaagtggtgt tttacatgca cttacaattg attaaagaca acactcacag
     4621 tgtggttatt tgttacacat aggggcgagc aatgtcatga cagtgtaggt gcggttactg
     4681 tcgtgaaaaa caataaaaac cgccattgca acaatggcgc aattcggatg aagcccctat
     4741 gacaaggata aaaataaacg cacgccgtat cttcagctta ttgattcctt ttttcttttt
     4801 cacttctgtt cacgctgaac aaacggcagc tcccgcaaaa cctgtaactg tggaagcgaa
     4861 gaatgaaacc tttgccccgc agcatcccga tcaatatctc tcctggaaag ccacctcgga
     4921 acagtcagag cgtgttgacg ccctggcgga agatccacgg ctggtgatcc tgtgggcggg
     4981 gtatcccttc tcgcgcgatt acaacaagcc gcgtggacat gcctttgctg tgaccgatgt
     5041 gcgtgaaacc ctgcgtaccg gtgcgccgaa aaacgctgaa gatggtccgc taccgatggc
     5101 gtgctggagt tgtaaaagcc cggatgtggc gcgtctgatc cagaaagacg gcgaagatgg
     5161 ctacttccac ggtaagtggg cgcgcggcgg cccggaaatc gtcaacaact taggttgtgc
     5221 cgactgccat aacaccgcct caccagagtt cgccaaaggc aaaccggagt taaccctttc
     5281 ccgtccgtat gcggctcgcg cgatggaagc cattggtaaa ccttttgaga aagccggacg
     5341 tttcgaccag caatcgatgg tttgcggtca gtgccatgtg gagtattact tcgacggcaa
     5401 aaacaaagcg gttaaattcc cgtgggatga cggcatgaaa gtcgaaaata tggagcagta
     5461 ttacgacaaa attgccttct ctgactggac taactccctg tcgaaaacgc caatgctgaa
     5521 agcgcagcac ccggaatatg aaacctggac agcgggcatt cacggtaaaa acaacgtgac
     5581 ctgtatcgac tgccatatgc caaaagtgca gaacgccgaa ggcaaactct acaccgacca
     5641 taaaattggt aatccatttg ataacttcgc ccagacttgt gcggactgcc atacccagga
     5701 caaagctgcc ttgcaaaaag tggtcgcgga acgtaagcag tcgattaacg acctgaaaat
     5761 caaggttgaa gatcaactgg ttcacgctca cttcgaagcg aaagcagcgc tggatgcagg
     5821 cgcgacggaa gctgaaatga agccaattca ggacgatatc cgtcatgccc agtggcgctg
     5881 ggatctggcg atcgcttccc acggcattca tatgcacgca ccggaagaag gtttacggat
     5941 gctcggtacg gcgatggata aagcggcgga tgcacgcacc aaactggcgc gcctgctggc
     6001 gaccaaaggc atcacccatg aaatccagat cccggatatc tcaaccaaag agaaagccca
     6061 gcaggccatt ggcctgaaca tggaacaaat caaggccgag aagcaggact tcatcaaaac
     6121 ggtgatcccg cagtgggaag aacaggcacg taaaaacggt ctgttaagcc aataaccccg
     6181 ttccgcctcg caaggggcgg aaaacacaat ggagtgaata tgagcgtatt acgttcgtta
     6241 ttaactgctg gggtgctggc gtcgggcctg ttgtggagcc tgaacggaat taccgctacc
     6301 cctgcggcgc aggcatctgg tgatcgctac gaagttaccc agcagcgtaa cccggatgcc
     6361 gcctgtctgg actgtcataa accagatacc gaaggtatgc atggcaaaca tgcttccgtt
     6421 atcaacccga ataacacatt gccggtcacc tgcaccaact gtcacggcca gccatcaccg
     6481 caacaccgcg aaggggtgaa agatgtgatg cgctttaacg agccgatgta caaggtcgga
     6541 gagcagaaca gcgtctgtat gtcctgtcat ctgccggaac agttgcaaaa agcgttctgg
     6601 ccgcacgatg tccacgtaac caaagtggcg tgcgccagct gccattccct gcatccatag
     6661 caagatacga tgcagacgtt aagtgacaaa ggacggatta agatttgcgt cgattgccac
     6721 agcgatcagc gcaccaatcc gaactttaac ccggcgtctg ttccgttgct taaggagcag
     6781 ccatgacctg gtctcgtcgc cagtttctca ccggcgtcgg cgtgctggca gctgtcagcg
     6841 gcaccgcagg gcgcgtggtg gcgaaaacgt tgaatatcaa tggggtgcgt tacggcatgg
     6901 tgcatgacga gtcgttatgc atcggctgta ccgcctgtat ggatgcttgt cgggaagtga
     6961 acaaggtgcc ggaaggcgtc tcgcgcctga cgattattcg tagcgagccg cagggcgaat
     7021 ttcctgatgt gaaatatcgc ttcttccgta agtcttgcca gcactgcgat catgcgccgt
     7081 gcgttgatgt ctgcccgacc ggtgcgtctt ttcgtgatgc cgctagcggg attgtcgatg
     7141 ttaacccgga tctctgcgtc ggttgtcagt actgcatcgc cgcctgtccg taccgcgtgc
     7201 gctttatcca tccggtcacg aagacggcgg acaaatgcga tttctgccgt aaaaccaatt
     7261 tgcaggccgg taagctgccc gcgtgcgttg aggcttgccc gacgaaggcg ctgacgtttg
     7321 gcaatctgga cgatcctaac agtgagattt cgcaactgct gcgccagaag cctacttacc
     7381 gctacaagct ggcgctggga accaaaccga agctgtaccg cgtaccgttt aaatacgggg
     7441 aggtgagcta atgacgcaga cttccgcatt tcattttgaa tcgctggtgt gggactggcc
     7501 gattgccatc tacctgtttt tgattggtat ttctgccggt ctggtgacgc tggccgtgct
     7561 gttacgtcgc ttctacccgc aggcgggcgg tgcagacagt acgttgctgc gcaccacgct
     7621 gattgtcggg ccgggcgcgg tgatcctcgg tctgttgatc ctcgtcttcc acctgacaag
     7681 accgtggacc ttctggaagc tgatgttcca ctacagtttt acctcggtga tgtcgatggg
     7741 ggtgatgctg tttcagcttt acatggtggt gctgatgctg tggctggcga aaatctttgg
     7801 acatgatttg cttgccctgc aacaacgctg gttgccgaag ctggggatcg tgcaaaaggt
     7861 tctgagcctg ctgacgcccg ttcatcgcgg actggaaacg ctgatgctgg tgttggcggt
     7921 gctgctgggg gcttataccg gctttctgct gtcggcgctg aaatcgtatc cgttcctcaa
     7981 taacccgatc ctgccggtgc tgttcctctt ctccggcatc tcgtccggtg cggcggtggc
     8041 gctgatcgcc atggcgatac gccaacgcag taacccgcat tccacggaag cgcactttgt
     8101 tcaccggatg gaaatccccg tggtatgggg tgaaatcttc ctgctggtgg cgttttttgt
     8161 cggtctggcg ctgggcgatg acggtaaagt tcgtgcgctg gtggcggtat taggtggcgg
     8221 tttctggacg tggtggttct ggcttggtgt cgccgggctg gggctgattg tgccaatgtt
     8281 gctcaaaccg tgggtcaatc gcagttccgg cattcctgcc gtgctggcgg cgtgtggggc
     8341 cagtctggtc ggcgtgttga tgctgcgctt tttcattctc tacgccggac agttgacggt
     8401 ggcgtaagcc agaaaagagg tggtttctgg acgtattcct tcctgaagtc ggttttctgg
     8461 cgttgttgtt aagtctcggg gtcaacgtgt tgaccccgtt gacggccttt gcgggagtgc
     8521 ggttgcgctg gcctgccatg atgcgactca cttgcatcgg cattctggcg cagttcgcgc
     8581 tcctgctgct cgcctttggc gtactgacgt attgttttct catcagcgat ttctcggtca
     8641 tttatgtcgc gcaacatagc tatagcctgc tgtcgtggga actcaagctg gcagcggtgt
     8701 ggggcggcca tgaaggttcg ctgctgcttt gggtgctgct gctttccgcc tggagcgcgc
     8761 tgtttgcctg gcattatcgg cagcaaaccg atccgctatt tccgctgacg ctagccgttt
     8821 tatctctcat gctcgccgca ctgctactgt ttgtagtgct gtggtccgat cccttcgtgc
     8881 ggatatttcc accagcaatc gaaggccgcg atctcaatcc gatgctgcaa catcccggtc
     8941 ttatctttca tccaccgctg ctttaccttg gctatggcgg tttgatggtg gcggcgagcg
     9001 tggcgctggc gagtttactg cgcggcgagt ttgatggtgc ctgcgccaga atttgttggc
     9061 gctgggcact acctggctgg agcgcattaa cggcggggat catcctcggt tcctggtggg
     9121 cctattgcga actgggctgg ggcggctggt ggttctggga tccggtggaa aacgcctctt
     9181 tattaccctg gctttctgcc actgcgctgc tgcacagttt gtccctgaca cgccagcggg
     9241 agattttccg ccactggtcg ctgttgctgg cgatagtaac tctgatgctg tcgctactgg
     9301 gtaccttaat tgtccgttct ggcattctgg tttcggttca tgcgttcgca ctggataacg
     9361 tccgcgccgt gccgttgttc agcctgtttg cactgattag ccttgcgtct ctggctctgt
     9421 atggctggcg agcgcgggac ggtggcccgg tggtgcgttt ttcggggtta tcgcgggaaa
     9481 tgttaatcct cgctacgctg ttgctgtttt gcgcagtgct actgatcgtg ctggtgggaa
     9541 cgctttatcc gatgatttac ggtctgctgg gctggggacg cctctccgtt ggcgcgccgt
     9601 attttaaccg cgcgacgtta ccgtttggcc tgttgatgct ggtggtgatt gtgctggcga
     9661 cgtttgtctc tggcaaacgc gtgcagcttc cggcgctggt agctcatgcg ggcgtgctgt
     9721 tatttgccgc tgggatcgtg gtttccagcg tcagccgtca ggagatcagc ctgaatttac
     9781 agccgggtca gcaggtgacg ctggcaggat acaccttccg ttttgagcgc ctcgatctgc
     9841 aagccaaagg caattacacc agcgaaaaag cgatagtggc actgtttgac catcagcaac
     9901 gcattggtga actgatgccg gagcggcgtt tttacgaagc acgtcgtcag caaatgatgg
     9961 aaccgtcaat tcgctggaac ggcatccatg actggtatgc ggtcatgggt gaaaaaaccg
    10021 gagcggatcg ttacgctttt cgtttgtatg tacaaagcgg tgtgcgctgg atctgggggg
    10081 gaggattgtt gatgattgcg ggcgcattgt taagcggatg gcgggggaag aagcgcgatg
    10141 aataaagggc ttctcacgct actgctgtta tttacctgtt ttgcgcgcgc tcaggtcgta
    10201 gacacctggc aattcgccaa tccgcaacaa cagcaacagg cgttaaatat tgccagccac
    10261 ttacgttgtc cgcagtgcca gaatcaaaac ttactggaat ccaacgcgcc ggtggcagtc
    10321 agtatgcgcc atcaggttta cagcatggtg gcggagggga agaacgaagt cgaaatcatt
    10381 ggctggatga ccgaacgcta cggagatttt gttcgctata acccaccgtt aacgggtcag
    10441 acgctggtgt tgtgggcgct gccagtggtg ttgttactgc tgatggcact gatcctctgg
    10501 cgagtgaggg cgaagcgatg aaacagccca aaataccggt gaaaatgctg acaacactca
    10561 cgatattgat ggtatttctc tgtgtcggca gttatctgtt aagcccaaaa tggcaggcgg
    10621 tacgtgcgga gtatcagcgt cagcgcgatc cgctacatca gtttgccagc aagcaaaccc
    10681 cggaagcgca gcttcaggca ttacaggata aaatccgcgc taatccacaa aacagcgaac
    10741 aatgggcgtt actgggcgag tattatctgt ggcaaaacga ttacagcaat tcgctgctgg
    10801 cgtaccgtca ggcgttgcaa ctacgtggtg agaacgctga actgtatgcg gcgctggcga
    10861 cggtgcttta ttaccaggcc agccagcata tgaccgccca gactcgcgca atgatcgata
    10921 aagccctcgc gctggactct aacgaaatca ccgctctgat gctgctggct tccgatgcgt
    10981 ttatgcaggc gaattacgcg caagccatcg aactatggca aaaagtgatg gatctcaact
    11041 caccccggat caaccggaca cagctggtcg agtcgattaa tatggcgaaa ttgttgcagc
    11101 ggagatcgga ttaatcatct ggtgatgaag attatgttat tgagctgcat aaaaaacaat
    11161 cgaatgaaca tatgccaaaa ataatcacta atcagtatta ttgcagatta aacaaataaa
    11221 aatctttcca taacaaatgg ttattcatta atcctgccat aaactcgcgt tattatgcat
    11281 taatgcagcg aaaagctctg ttgttaaagg gttgcgcaac ataccgcgca aatgatactg
    11341 atcataagcg ttaaaaaaat ctacaaacca acgcaacaca attcatgccc tggcagtatg
    11401 tcacgttctc gcgtttctga acggggaacg gcgctccatt gaggaagtta ttctgatgaa
    11461 aagtataaaa ttcagcctgg cctggcagat tctgtttgct atggtgctgg gcattctcct
    11521 gggaagctac ctgcactacc atagcgacag ccgcgactgg ctggtcgtca atttgctctc
    11581 tccggcgggt gatatcttca tccatctgat caaaatgatt gttgtgccga ttgtgatctc
    11641 cacgctggtg gtgggtatcg cgggtgttgg tgatgccaaa cagctcgggc gtattggcgc
    11701 gaaaaccatt atttacttcg aggtgatcac caccgtcgcc atcattttgg ggatcactct
    11761 ggcgaacgtc ttccagcccg gtgccggggt ggatatgtcg cagctggcga ccgtcgatat
    11821 ctcgaaatat cagagcacta cggaagcggt acaaagcagt tcccacggca ttatgggcac
    11881 gattttgtcg ctggtgccga cgaacattgt ggcgtcgatg gcgaaaggcg aaatgctgcc
    11941 gatcatcttc ttctcggtgc tgtttggtct ggggctttcc tccctgcccg cgacgcatcg
    12001 tgaaccgctg gtgaccgtgt tccgctccat ctctgaaacc atgtttaaag tgactcacat
    12061 ggtgatgcgt tatgcaccgg tgggtgtgtt tgcgctgatt gcggtgacgg tggctaactt
    12121 tggtttctct tctctgtggc cactggcgaa actggtgctg ctggtgcatt tcgccattct
    12181 gttcttcgcg ctggtagtgc tgggaattgt ggcgcgcctg tgcgggttaa gcgtctggat
    12241 cctgattcgt attctgaaag atgagctgat tctggcgtac tccactgcca gctctgaaag
    12301 cgtactgccg cgaattattg agaagatgga agcctacgga gcaccggcgt cgatcaccag
    12361 cttcgtggtg ccgaccggtt actcttttaa ccttgatggt tcgacgctgt atcaaagtat
    12421 tgccgctatt ttcatcgctc agctgtatgg cattgacctg tccatctggc aggaaatcat
    12481 tctggtgctg acgctgatgg tgacctcgaa agggattgct ggcgtgccgg gcgtgtcgtt
    12541 tgtggtgctg ctggcaacgc tgggtagcgt aggtattccg ctggaaggcc tggcgtttat
    12601 tgctggtgtt gaccgtatcc tcgacatggc gcgtactgcg ctgaacgtgg tgggtaatgc
    12661 gctggcggtg ctggtgattg ccaagtggga acacaaattt gaccgcaaaa aggcgctggc
    12721 ttatgagcgt gaagtgctgg gcaaatttga taaaactgcg gatcaataat tgaagattgc
    12781 cggggttatc cacccggcaa tgtgtggatg cctgatgcga cgctggtgcg tcttatcagg
    12841 cctaccttaa ccgttggtta atttttcaaa ctcttcacac ccggtatcaa acccttccat
    12901 acagctcagg ttcaaccagt gcagcgcctt ttgtttgttc ttctcgataa aaccttcttc
    12961 accgtttaag aacatcattc ccgcccagta ctcggaataa ccggttcggg aaattgcaga
    13021 gctgcgtttg aaataccagg ttgccttgtc atcgtcggcc ttaatgccca cgccgttggc
    13081 gtaaatcaaa ccaagcagca tttgggcatc gacggcagag tcgttctcca gatcttccga
    13141 ggcgttttct aacagcgaaa tcgcctttgg ataatccggt ttacccgctt gagtatttac
    13201 cagaatatgc gccagcgtta cttcaccttc tttactgccc gcttgcgccg ctttctctgc
    13261 aagaactttt gcttgtggat agtccagact gaccggattg gtgattttga tctgcgccaa
    13321 cagcgcgcag gcatcagcat caccgctgtc ggcggctttc tgtgcccaat attcggcttt
    13381 gctcaaatcg ccggagctaa accagctgtc ggcaagaaaa tattgtgcgc gtcggtcccc
    13441 ggcctctgct gcctttaaat actggctgcc aggctcggag tcgttggcgt gggcaaagaa
    13501 tgtcaaaaac aacatcaatg cgataatttt tttcattttg ataatacgat tgggtaggga
    13561 gtaaccagta taaagagttc gaatggataa aaaaacagcc tccgaaagga ggctgtagaa
    13621 aggactgtat tacgccagtg ccgcttcgcg caggcgagtt ttcaacttgt tgtactcgtc
    13681 aatcacgtac tgctcggcgg cgcgctgatc ggcgatcggc tcgacgcgca cggcgcagta
    13741 tttgtattcc ggcgttttcg taatcgggct taagttttcg gtaaccagct cgttacaggc
    13801 accaatccac cactggtagg tcatgtaaat cgcccctttg ttcggacgat cgctgacctg
    13861 cgcacgggtg ataattttgc ctttacgcga gtgcacccaa accaatgcct catcttcaat
    13921 acccagacgt ttggcgtctt cggtattgat ttgtgcgtag ccaggttcat cagccagcgc
    13981 cgccagtgcc gcacagttac cggtcatcga acggcaagag tagtgaccaa cttcacgcac
    14041 cgttgacagt accatcgggt actcgtcggt gagtttgtcg attggcgcta cccagtcgca
    14101 ggtgaagaac tgcgccagac cgttcggggt atcaaacttc tctttaaaca gataagaagt
    14161 cccctgatcg gcatctgaag tatcgcggca aggccactga atgaagccca gttcgcccat
    14221 tttctcgtaa gtcgcaccgt agaaatccgg gcacagatga cgcaactcat cccagatctc
    14281 ctgggtgttg ttgtagtgca tcggataacc catacgggtg gcgatttcac tgatgatttg
    14341 ccagtccgtt ttcagatccc atttcggttc aactgctttg aagaaacgct ggaagccacg
    14401 gtcagccgca gtaaacacgc cttcatgctc gccccacgac gttgacggca aaatgacatc
    14461 cgccgccgac gcggttttgg tcataaagat gtcctgaacg ataaccagtt ccagatcttc
    14521 aaaggcttta cgtactgccg acagctccgc atcagtttgc agcggatctt cgcccataat
    14581 gtacgcggca cgcacttcgc catgcgctgc gcggtgcggc agctcgctga tgcgataacc
    14641 ggtatgagca ggcaggcttt ccacgcccca ggctttggcg aatttctcgc ggttagccgg
    14701 atctttcacg tactggtatc ccggatacgt atccggcagc gcgcccatat cgcaggcacc
    14761 ctgcacgttg ttctgaccac gaaccgggtt tacacccgca tgcggcttac cgaggttacc
    14821 gctcagcatc gcgaggctgg tcagagaacg cacggtttcc acgccctggt agaactgggt
    14881 tacacccatg ccccacagga tggcggcgct tttcgcctgg gcatacatcc gtgccgcctg
    14941 acgaatctca ctggcgctga cgccggtgat atcttcaacc gactccggcg tgtagccttc
    15001 aacgatttta cgatactctt caaagccttc tgtacgggaa gcgacgaacg ctttgtcgta
    15061 cagattttct tcaataatga catggcccat cgcattcaac agcgcgatgt tcgagccgtt
    15121 tttcagtgca atgtgcatgt cagcaatgcg cgcggtttca attttgcgcg gatcgcagac
    15181 gataattttc gccccgttac gtttagcgtt aattacgtga ttcgccacga ttgggtggga
    15241 atccgccggg ttgtacccga aaacgaacac taaatcggta ttatcaattt cgttaatagc
    15301 attgctcatt gcgccattac cgaccgattg gtgcagacct gcaaccgatg ggccgtgtca
    15361 gacacgagcg cagcagtcaa cgttattggt accaataacg gcgcgcgcaa atttttgcat
    15421 tacatagttg gtttcgttac ccgtaccacg cgaggagccg gtcgtctgga tggcatccgg
    15481 accgtacttc tctttgatgg cgctcaggcg ctcggcaacg taattcagtg cctcatccca
    15541 ggaaacaggt tcgagtttgc cgccacgctg gcgacggatc atgggggttt tcaggcgcgg
    15601 ggtcaggatc tgggtatcgt taatgaaatc ccagccataa taacccttca gacacagggt
    15661 accctggttg gttttcccct gcgctgcctc cgcccggacg attttgccgt tatcgacgac
    15721 caggttgatt ttgcaacctg atgcgcaata ggggcaaacc gtgacgactt ttttcatcgg
    15781 tctcgctcca gttaatcaaa tcacgcatac gcgctctcga ctacagtatg catcttttat
    15841 gccacatttt atttggggtc attccctgat attacgggca ctatttattc aaaactctga
    15901 cgaaaaacag gctgtcgtca gttttgacgt gacgaaacga aataccgcgt gacagccatc
    15961 acgcggcaga cattttattt tttctcgacg acgggagccg cctgataccc gccgcccagc
    16021 gatttcatca actgaatgct ttggatcacc cggcggctgt ccagcatcag taatgacatc
    16081 tcttcggcaa gtactggcaa ccgggcttca gtggcctgta agcggctggt taagccgcgc
    16141 tgataggcgg cctcggcagc gcgctgggtg aagcgcgttg cttccacgtg ttcagcctgc
    16201 atttctcgct cgtcgttgag cgtttgcaga cgcgtgccgt tgacggcaac gtcacgcacc
    16261 gcgttcagta ctgactggtt gtaacgttca atcatcatgt tgctggcggc gcgcgtgcct
    16321 tcgagattgg cattcaaccg tccaccgtca aacagcggca atttcagacc cgggatgaag
    16381 ttgaactggc gactggtttt tttgaataag gtatccaggt ggatggcatc aaggccgaaa
    16441 aacgctttga tatcaaagct cgggtagaac agcgcccgcg cggaatccac ctgatctaat
    16501 gacgcctgaa cataccagcg catggcttgc agatccgggc gtctggcgag caactcataa
    16561 gagagtgtcg ccggaatgcc ggtctggact cgtggtaatg ccaccggttt gatctccggc
    16621 atatcgctgg cccccgcgcc aatcaatgca cgcagagatt ctcgcgtttc ggtgatttgc
    16681 cctttgacgg cagcaatttg tttatcgacc gccagaatct gtgcccgcgc gccgtggaaa
    16741 ggcacttgtg cttccagacc gtgcgccact ttactctggt gcgctttcac cgcgtaatca
    16801 atcacatcgc gagtttgttc taacagatcg agcatctgat agctggcctg catactgtaa
    16861 taaagctgcg ctacgcccgt ggtcagcgat agctctactg ctgcggtttc tgccagcgcg
    16921 gcattatgcg cgccaatggc ggcggcaacc gctgagcgat gcacacccca caaatcaaga
    16981 tccagtccgg caaacaaacc tactgtggct tccgtatagt acggcccgtc catacccagt
    17041 gcgggcgcat ccatcgcata agggcttaaa aagccgttcg ccgagacgcg ttggcgcttg
    17101 agcatcccta acgctgcgac ctgtaattgt gaaccggcat ctaacaaatc ggcctgggac
    17161 tgcgcttttt cttcccgcag tttcgcttcg gcgagggtgt gtgaaccgct tagcgtccgt
    17221 tggatcagcg catccagctg cgggtcattg agttgtttcc accactgcgc ctgcggccag
    17281 ccggagctgg cacgatgaat atcgtcggcc agtttgattt gttccggttt gagctgttga
    17341 tgaggtgcag aatccttacg taccagggca cagccggaaa tcagcgtcgt gctgccgaga
    17401 atgctgcaca acagcagacg tgaaagttga cgattgatca ttgcgtggct ccttgtgcct
    17461 gttccgtggc gggcgttaat gccggggctg tccagtctgg caagcgggcg aaaagctggc
    17521 agacgtgttg ctcctgtttt aataaggtgg gcaggatggc ctgcgaggcg ggtgtctctt
    17581 caagcgttat ttgaggagaa cggctgagtg cggttgccag accggcagcg tatttctcca
    17641 gggcgtcggc aaactgacct gctctgtccg gctgtagtgc gttatccagc gcctgcgccg
    17701 agttccaggt ggcatcccag gcgtgaagaa tatcgcggcc ctgacgaata accgtttgcg
    17761 aacgttcaat cagtaaggcg cgttcttcgc tgtccagttg acgctccagc gccacgcgtt
    17821 ggcacatctc ttcacaggca ttaaacgccg catgcagacc gatacgaatt tgcagataag
    17881 tgcgcagagc cgtgacttcc tgctggcgtg ggatccgcat tactttactt aacataccca
    17941 gcgtgccagc cagtttttgc ggcagtgtgc gcgcttcact ttcaggccag acaaaggtgt
    18001 aaatcaccgc ggacaccacg gtaccaatga tgatacccag ggcgcgatcg cgaatttcca
    18061 ccaggtcgta cactgggcca aaaacgtttt cgagcgtggc gagcgcgaag gtgaccacca
    18121 tctgtgtgcc gatataagaa gagcgttcag agctggtggc aatccatgcg cccaacagga
    18181 aaatcggtgc cagcacaaac agcaattcga caatattgtc cagccagggc atgaccagta
    18241 gcgtgaataa cagcgccaga atcgcgccgc aaaaggcccc gccaaaacgc agcaccatct
    18301 tctggtacga cgaaccgaca tttgggttag cgacgatcac gcatgtcagc atacaggtgt
    18361 gaatgccttc ccagtccacg ccgctgtaaa aggtgtaaca gatcaaacag gcgagcagcg
    18421 tttttaccgc gtagcgcata tagtctggat tggtaaaagc atcggcggcc attgatggcg
    18481 gtttggctgc gggcgttggc ggcgtattcg ggtccatctg acccagttgt aacaacgtct
    18541 ggcagatatt ctccaggtta cattcccgtg ccgccatcgc ttcactttca ctgatccgcc
    18601 agtcgctttg ccagcactga ccttcagcaa cggcatgctg cagcttattg atttctgagg
    18661 ctaatttttg ccgaaattca ataattgcct gagaatcagc aaaagaggtg ggatcgtagc
    18721 gattcagcgt cgagtaaatg taggttaccg ttgccacgca gctttgccac catgcgcttt
    18781 gagttcgcca gttggcatcg tccgcgagac aaaagacatt cagtttttgc agcgccagcg
    18841 cctctctttc aatccgcgtt tcgggtagcg gtgcgaggct gtccgtcagg tggctaatgg
    18901 catcatcaag ccgatcatta agcgcctgat gcatttgcgt aatggcacga ctgggaaacc
    18961 acagcacgcc gattaacgtc atcagcaagg ttggataaag gccaacaacg atacaccaca
    19021 gcgttaagcg cacgaccact tccggatagt cgagcatggc ggggaaggtt tgcccgtaaa
    19081 tggcgacaat ggcgacggcg aaaaagacca gtcccagccg atgggtgcgc atcaaaaaca
    19141 tacagcccat caggatcggc ccggcgatga ccaatcggat caacggttcg ccgtatgacc
    19201 atttgtagat caaaaacagg ctgccgatct ccagcatcgt ggcgaccaca aataagatcg
    19261 cgacaaattt ggtgtaaaac gcgttcgact gaataccgta aaacagcact gccagcgata
    19321 acgccacgaa agggatctca aaggtcatcg agatcagaat caccagcagg cagcctaccc
    19381 agagttgcac ggtctgcggc acgcggcctg gtcgccgctc gcttaactct tcatgaaaga
    19441 acgccagcag cctgaccacc ggtaatggca gggagttgag cgcgctcatt attgcggctc
    19501 aagattagcg actgccgaag cgccgatgcg gaacatttcc gggtcaggtt tatcgaccat
    19561 gattttgacc ggaaaacgct gggcaacgcg gacccagtta atagagcgag aaactttcgg
    19621 caggccgccc agcaccaggc cgccgtcatc cggtagcacg ccgtagccaa tcgaatccac
    19681 tttaccctcg aaggttttgc cgctgtcact catcaggcga atcgttgcgg gtgtacctga
    19741 gcgaatattt ttcagatcgg tttcgcggaa gttggcgatc acgtaccagt aacgagtgtc
    19801 gattagggta aaaatagggc gcatggcaga agcaaattgc ccgacggagg ttttgaggga
    19861 aatgacccgg ccatcaaacg gcgcgcgaac ggtcgccatt tccagatgaa gtttggtcag
    19921 ggcaatatcc gcttcgaccg ccgcacgttg cgccaccagc gcatcgacgc cgctgacggc
    19981 gctggcggct gactgcgcct gtaacaatac ggcattaaga tcggcttctg cggcgcgctg
    20041 cgcagttctt gcacggtcaa cgtcttccgc tgagacaaaa ccctctttca gtaatggctc
    20101 ggtgcggcgt aatgtatctg tggcctgttt cgcggcggca cgggcttttt ctaccgtggc
    20161 attaaccgag tcggcaccaa actgttgcgc atcaacgcta cgctgggtga gcataatttg
    20221 cttatccagc gccgcgaggg aggcttcagc tttcgccaga ttggcttcgt acgggcgcgg
    20281 gtcaatgcgg aacagcaaat cgccctgttt gactgcctgg ttgtcggtga ccgctagttc
    20341 tataatgcga ccgctgactt ccggcaccac atcaatggta tctgctgacg cgtaagcgtc
    20401 attagttgat ggcgcactgt ctacgcgcca gataacgaaa acaagggcaa ccagcgccaa
    20461 cgcaaccact aatagagcag ggaatttact gcgaggagct tttttcggcg tactttccat
    20521 gagcgcgtct caaatagatt agaaaaatgc cagccagaac agcatggcgt agagagcaaa
    20581 aagggcggta taaataattc cgacaaatgc cagattgata tttgcccgct gaataactcg
    20641 cctcgtgatg agcgtcaaaa taaggctggc aatggcgcag aaaaaccagc tgggataata
    20701 agcgccgatc accgggattg ccggagataa tgagcatccc gaaaccatga agacgggaat
    20761 tatccaggct gtttttttga gttgcatcgc catgcgagag agaactgttg gcatattaaa
    20821 tacatcctgt ctgctgcgaa caatatctgt agtgaatatc aacatccatt gaaggcgtca
    20881 gtatggctat tttcttgatg aataaa
//
LOCUS       DAZKHC020000074        20447 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZKHC020000074 DAZKHC020000000
VERSION     DAZKHC020000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.074"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(207..953)
                     /gene="cutC"
                     /locus_tag="ACHOSM_RS16430"
                     /old_locus_tag="pgaptmp_003286"
     CDS             complement(207..953)
                     /gene="cutC"
                     /locus_tag="ACHOSM_RS16430"
                     /old_locus_tag="pgaptmp_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGU4559746.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRVEKIMAAAGPLAVTFHRAFDMCANSLNTLNNLAELGIVRVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(967..1533)
                     /gene="yecM"
                     /locus_tag="ACHOSM_RS16435"
                     /old_locus_tag="pgaptmp_003287"
     CDS             complement(967..1533)
                     /gene="yecM"
                     /locus_tag="ACHOSM_RS16435"
                     /old_locus_tag="pgaptmp_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4559747.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1749..3482
                     /gene="argS"
                     /locus_tag="ACHOSM_RS16440"
                     /old_locus_tag="pgaptmp_003288"
     CDS             1749..3482
                     /gene="argS"
                     /locus_tag="ACHOSM_RS16440"
                     /old_locus_tag="pgaptmp_003288"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGU4559748.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWMEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3524..3568)
                     /locus_tag="ACHOSM_RS16445"
                     /old_locus_tag="pgaptmp_003289"
     CDS             complement(3524..3568)
                     /locus_tag="ACHOSM_RS16445"
                     /old_locus_tag="pgaptmp_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559749.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            3659..4147
                     /gene="yecT"
                     /locus_tag="ACHOSM_RS16450"
                     /old_locus_tag="pgaptmp_003290"
     CDS             3659..4147
                     /gene="yecT"
                     /locus_tag="ACHOSM_RS16450"
                     /old_locus_tag="pgaptmp_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HGU4559750.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAHGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4269..4661)
                     /gene="flhe"
                     /locus_tag="ACHOSM_RS16455"
                     /old_locus_tag="pgaptmp_003291"
     CDS             complement(4269..4661)
                     /gene="flhe"
                     /locus_tag="ACHOSM_RS16455"
                     /old_locus_tag="pgaptmp_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HGU4559751.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4661..6739)
                     /gene="flhA"
                     /locus_tag="ACHOSM_RS16460"
                     /old_locus_tag="pgaptmp_003292"
     CDS             complement(4661..6739)
                     /gene="flhA"
                     /locus_tag="ACHOSM_RS16460"
                     /old_locus_tag="pgaptmp_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HGU4559752.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATIDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6732..7880)
                     /gene="flhB"
                     /locus_tag="ACHOSM_RS16465"
                     /old_locus_tag="pgaptmp_003293"
     CDS             complement(6732..7880)
                     /gene="flhB"
                     /locus_tag="ACHOSM_RS16465"
                     /old_locus_tag="pgaptmp_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HGU4559753.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLSGIKRMFSAQTGAELLKAILKTILVGSVAG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8082..8726)
                     /gene="cheZ"
                     /locus_tag="ACHOSM_RS16470"
                     /old_locus_tag="pgaptmp_003294"
     CDS             complement(8082..8726)
                     /gene="cheZ"
                     /locus_tag="ACHOSM_RS16470"
                     /old_locus_tag="pgaptmp_003294"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HGU4559754.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRKFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8737..9126)
                     /gene="cheY"
                     /locus_tag="ACHOSM_RS16475"
                     /old_locus_tag="pgaptmp_003295"
     CDS             complement(8737..9126)
                     /gene="cheY"
                     /locus_tag="ACHOSM_RS16475"
                     /old_locus_tag="pgaptmp_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HGU4559755.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9141..10190)
                     /gene="cheB"
                     /locus_tag="ACHOSM_RS16480"
                     /old_locus_tag="pgaptmp_003296"
     CDS             complement(9141..10190)
                     /gene="cheB"
                     /locus_tag="ACHOSM_RS16480"
                     /old_locus_tag="pgaptmp_003296"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HGU4559756.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10193..11053)
                     /gene="cheR"
                     /locus_tag="ACHOSM_RS16485"
                     /old_locus_tag="pgaptmp_003297"
     CDS             complement(10193..11053)
                     /gene="cheR"
                     /locus_tag="ACHOSM_RS16485"
                     /old_locus_tag="pgaptmp_003297"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HGU4559757.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHACRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11072..12673)
                     /gene="tap"
                     /locus_tag="ACHOSM_RS16490"
                     /old_locus_tag="pgaptmp_003298"
     CDS             complement(11072..12673)
                     /gene="tap"
                     /locus_tag="ACHOSM_RS16490"
                     /old_locus_tag="pgaptmp_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HGU4559758.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQAAWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12719..14380)
                     /gene="tar"
                     /locus_tag="ACHOSM_RS16495"
                     /old_locus_tag="pgaptmp_003299"
     CDS             complement(12719..14380)
                     /gene="tar"
                     /locus_tag="ACHOSM_RS16495"
                     /old_locus_tag="pgaptmp_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HGU4559759.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSISHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14523..15026)
                     /gene="cheW"
                     /locus_tag="ACHOSM_RS16500"
                     /old_locus_tag="pgaptmp_003300"
     CDS             complement(14523..15026)
                     /gene="cheW"
                     /locus_tag="ACHOSM_RS16500"
                     /old_locus_tag="pgaptmp_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGU4559760.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15047..17011)
                     /gene="cheA"
                     /locus_tag="ACHOSM_RS16505"
                     /old_locus_tag="pgaptmp_003301"
     CDS             complement(15047..17011)
                     /gene="cheA"
                     /locus_tag="ACHOSM_RS16505"
                     /old_locus_tag="pgaptmp_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HGU4559761.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKASEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(17016..17942)
                     /gene="motB"
                     /locus_tag="ACHOSM_RS16510"
                     /old_locus_tag="pgaptmp_003302"
     CDS             complement(17016..17942)
                     /gene="motB"
                     /locus_tag="ACHOSM_RS16510"
                     /old_locus_tag="pgaptmp_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HGU4559762.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FKTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17939..18826)
                     /gene="motA"
                     /locus_tag="ACHOSM_RS16515"
                     /old_locus_tag="pgaptmp_003303"
     CDS             complement(17939..18826)
                     /gene="motA"
                     /locus_tag="ACHOSM_RS16515"
                     /old_locus_tag="pgaptmp_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HGU4559763.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18953..19531)
                     /gene="flhC"
                     /locus_tag="ACHOSM_RS16520"
                     /old_locus_tag="pgaptmp_003304"
     CDS             complement(18953..19531)
                     /gene="flhC"
                     /locus_tag="ACHOSM_RS16520"
                     /old_locus_tag="pgaptmp_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HGU4559764.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19534..19884)
                     /gene="flhD"
                     /locus_tag="ACHOSM_RS16525"
                     /old_locus_tag="pgaptmp_003305"
     CDS             complement(19534..19884)
                     /gene="flhD"
                     /locus_tag="ACHOSM_RS16525"
                     /old_locus_tag="pgaptmp_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HGU4559765.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4938 a         5598 c         4962 g         4949 t
ORIGIN      
        1 tcagttttag caatttaaat cacataatta atgtgaatat ataaaatatt tttagcacaa
       61 cgctatgtgc actgtcaccc gctcaacaga tcgcggtaat cgttcatttt ttacactatt
      121 cagggataaa aaaaggcccc tgttgaaatt gcaggggcct ggtacgagca agcatcatat
      181 tgggcgacat gatgcaacgg taaaaatcat ttggcctgat ggcgttcaat gattcctttc
      241 atttcagcaa ccgccgcccc gtctacgaca taacgcgaat actcgtccgc gtgtgcatct
      301 gatgacatgg acaatccttg attacgataa cgcatcggtg aggcttgcca cgctcccgcg
      361 gagctatgga cttccagcac tccggcatcg aggaagtggt gcaggttttc tgcacggact
      421 cctgctccgg ccataatgat tggagcatca cgatgggcaa taagttccat aatttttgat
      481 aaaccttgca gtgcgtctga tttttgccct gatgtcagca cccggacaat gcccaattcc
      541 gcaagattat tgagtgtatt tagagagtta gcgcacatat caaaggcgcg atgaaaagtc
      601 actgccagcg gaccggcagc agccattatt ttttccaccc gtggcatatc gacattcccg
      661 tcaacatcga gcacgcccgt taccagtcca ggaaaaccta attcgcggac tgtgcgcaca
      721 tcctcaagaa tggcggcaaa ctcaccgtcg ctgtagcaaa aatcaccacc gcgtgggcgg
      781 ataatcggat gcacagggat cgtcacccgc tggcgcacgg atttcagtac acccaacgac
      841 ggcgttaagc ccccctcttt tggggctgcg cataattcaa ctctgtctgc gccgttttgc
      901 tgcgccgtta gtgcacattc catgctgtaa cagcaaattt ccagtaatgc catttttact
      961 ccttaattac gccgactgct cgctggcaac gatctcttca atggaccacg gatgaaactt
     1021 aatagttgtt ttgccatcgg tgaccgccag cgtcggattc ggtaatcgct catgctcacc
     1081 ttttggcgaa ctggttttaa ctgagatccc cggtaggctg agtccttcat cagaaagcaa
     1141 tgccagcgca cgggcattca gcgtttctgg atcacccggc agaacgattt caatatgttc
     1201 ccaaccttcg tgcgggtaac gtttttcccc gggccacggt agctccacaa tggaaaactg
     1261 ccagtgagca acctgtaccg gttcatgcaa tttaaacaga caaatcggtc tgccattgat
     1321 catattttct gacaaaagct cgccgcactg ttcaaacccg cgacgccagc gttcagcggt
     1381 ggcgttttga tggcaacgca aggaaatgtg atcggcagtc aacggagtga tatccaaccc
     1441 caaacggcgg gaaagttcat ctaatgcgtg ggtaaatcgc ggtaaatccg atgcaatatc
     1501 ctgcagctcg tcgatagatt gccagttcgc cataatcact cttcgtcttt cagtaaaagc
     1561 gttaatttac cctgttgccc tgcgccaacc aaccgctgat ttcacgccgc ttctgatgca
     1621 atagtgaaaa cggcaatacg gcacgcgcac gttgctgacg aaaacagcca tttgcagtat
     1681 actcccgccc taatttcttt aactggtgcg ggcaattttt gctcgcttca tcaatgtaag
     1741 gtattccggt gaatattcag gctcttctct cagaaaaagt ccgtcaggcc atgattgcgg
     1801 caggcgcgcc tgcggattgc gaaccgcagg ttcgtcagtc agcaaaagtt cagttcggcg
     1861 actatcaggc taacggcatg atggcagttg ctaaaaaact gggtatggca ccgcgacaat
     1921 tagcagagca ggtgctgact catctggatc ttaacggtat cgccagcaaa gttgagatcg
     1981 ccggtccagg ctttatcaac attttccttg atccggcatt cctggctgaa catgttcagc
     2041 aggcgctggc gtccgatcgt ctcggtgttg ctacgccaga aaaacagacc attgtggttg
     2101 actactctgc gccaaacgtg gcgaaagaga tgcacgtcgg tcacctgcgc tctaccatta
     2161 ttggtgacgc agcagtgcgt actctggagt tcctcggtca caaagtgatt cgcgcaaacc
     2221 acgtcggcga ctggggcact cagttcggta tgttgattgc atggatggaa aagcagcagc
     2281 aggaaaacgc cggtgaaatg gagctggctg accttgaagg tttctaccgc gatgcgaaaa
     2341 agcattacga cgaagatgaa gagtttgccg agcgtgcacg taactacgtg gtaaaactgc
     2401 aaagcggtga cgaatatttc cgcgagatgt ggcgcaaact ggtcgacatc accatgacgc
     2461 agaaccagat cacctacgat cgtctcaacg tgacgctgac ccgtgatgac gtgatgggcg
     2521 aaagcctcta caacccaatg ctgccaggaa ttgtggcgga tctcaaagcc aaaggtctgg
     2581 cagtagaaag cgaaggggcg accgtcgtat tccttgatga gtttaaaaac aaggaaggcg
     2641 aaccgatggg cgtgatcatt cagaagaaag atggcggcta tctctacacc accactgata
     2701 tcgcctgtgc gaaatatcgt tatgaaacac tgcatgccga tcgcgtgctg tattacatcg
     2761 actcccgtca gcatcaacac ctgatgcagg catgggcgat cgtccgtaaa gcaggctatg
     2821 taccggaatc cgtaccgctg gaacaccaca tgttcggcat gatgctgggt aaagacggca
     2881 aaccgttcaa aacccgcgcg ggtggtacag tgaaactggc cgatctgctg gatgaagccc
     2941 tggaacgtgc acgccgtctg gtggcagaaa agaacccgga tatgccagcc gacgagctgg
     3001 aaaaactggc taacgcggtt ggtattggtg cggtgaaata tgcggatctc tccaaaaacc
     3061 gcaccacgga ctacatcttc gactgggaca acatgctggc gtttgagggt aataccgcgc
     3121 catacatgca gtatgcatac acgcgtgtat tgtccgtgtt ccgtaaagca gaaattgacg
     3181 aagagcaact ggctgcagct ccggttatca tccgtgaaga tcgtgaagcg caactggcag
     3241 ctcgcctgct gcagtttgaa gaaaccctca ccgtggttgc ccgtgaaggc acgccgcatg
     3301 taatgtgtgc ttacctgtac gatctggccg gtctgttctc tggcttctac gagcactgcc
     3361 cgatcctcag cgcagaaaac gaagaagtgc gtaacagccg tctgaaactg gcacaactga
     3421 cagcgaagac gctgaagctg ggtctggata cgctgggtat tgagactgta gagcgtatgt
     3481 aatcgatttt tcgtgagagt gaagcctgat cagggttagc cgatcaggct tttttattgc
     3541 catctaaatg tattctgaaa acggacatgc catcgttttc tcactgttga gtaagaggcc
     3601 agaagcgtaa tatccaggcc caggaaaacg ataacggttc aatttaagga atgccgcagt
     3661 gtttaaattt cttgtattaa cattaggcat tatctcttgc caggcttacg cagaagatac
     3721 ggttatagta aatgatcatg acatttcagc catcaaagat tgttggcaaa aaaattcaga
     3781 tgatgatact gacattaacg tgatcaaatc atgtctgcga caagaataca atctcgtcga
     3841 tgcgcaatta aataaagctc atggtgaagc ttatcgttat atagaacaag tgccacgcac
     3901 aggtgcaaaa aaacctgata ctgaacaact taacttgctt aaaaaatcac agcgagcctg
     3961 gctggatttt agggacaaag aatgtgaatt aatcctttca aatgaggatg ttcaggattt
     4021 aagtgaccct tattctgaat cagaatggct atcatgtatg atcatacaga ccaatacgcg
     4081 aactcgccag ttgcagctat accgtaactc tgaagatttt tatccaagcc ctttgacaag
     4141 aggataattc acatcttttt ggcatgtttt gttgcaagct attcctgata aataattgca
     4201 acaaaacatc gagccttttt cgctgagtta ttaaacatgc tcgcgagcgc gtaattttta
     4261 tttgtccttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
     4321 aaccgcccac cgccaggcac ttcccagata aagcgcaacg gttctgctgc cgctataccg
     4381 gaaaaggcca cggtggttcc gctctgccca tctaattcga cacaacgaga ttgcgaacac
     4441 aagcgaaccc gcagtcctgc aggtgtcggg ccgataagct gataccgcca cgccaccagc
     4501 gtcatcaatc ctgaagcagg ttgtcgagta gaaagaggcg ctgacgacat cgactcaccg
     4561 cgatgattta gcgtaattcc gacactactt gcctgccaca tcccctcccc ggcggcttgc
     4621 actagcagcg gaaacaataa tattgctaat aaggctctca ttatttgccg ccaatcgtcg
     4681 ccgtcatacg gatatgtcgg ttatctgata gttccagatt cgacagcacc actaactgcg
     4741 gcaagctgcg gcgcagaaag cgagacaata atggtcgtag cgcgtggttc accaacaata
     4801 ctggcggcgc acccaacatc tcctgacggg atagcgcttc ctgagtttgc gccagtaaac
     4861 gatccgccaa ccctggctcc agtccgcccc cgccctgcag cgcctgtagt aacaaacgtt
     4921 ccagcggtgt atcgaggcca ataacatgga cttcatcttt gccaggaaac cactgctggg
     4981 taatcgcccg tcccaacgcc acgcgcacga cggcggttaa ttcatgtgga tcactttgga
     5041 tgggtgcatg ttccgccagc gtttcgagaa tagtgcgcat atcgcgaatc ggcacttttt
     5101 catcgagcag attttgcagc actttatgca gtgtggtgag cgtgacgacg ccaggaacga
     5161 gatcttccgt cagctttggc atctcctggg cgacgcgatc cagcagctgt tgcgcctcct
     5221 gacgaccaaa cagctctgcg gcatgctggc taatgaggtg gttaagatgc gttgctacca
     5281 ccgtgctagc ctcaaccacg gtgtacccct gaatctgcgc ctgttctttt agcgcacttt
     5341 caatccagat agcattcagg ccaaatgccg gatcgatggt cgcctcacca ggtaacgtcc
     5401 cggcagcggt tccagggtta atcgccagcc agcgccccgg ataagcatca ccactgccta
     5461 tctccacgcc tttcatcaaa atgcgatagc gggcaggttg cagatccata ttgtcgcgaa
     5521 tgtgcaccac cggcggcaag aagcccattt cctgagcaaa tttcttacga atactacgaa
     5581 tacggcccag caactcgcca tcttgttgga aatcaaccat cgggatcagt cgataaccca
     5641 cttccattcc cagagaatct tccagttgta catcgttcca cgtcgcttcg acaacggtat
     5701 tattctctgc catttttacc ggtttgggtt cggcaggcgc tttttgttcg cgtccgcgta
     5761 tccaccaggc cagcccgagc aatccggcag tgaacagcaa aaataccagg ttcggcattc
     5821 caggcaccag gccgagtaaa ccgagcacgg cggcgcttaa caacataacg cttgggttac
     5881 tgaaaagctg attcaccatc tgctcgccaa catcctgatc ggtgctgaca cgcgtaacga
     5941 tgacccccgc ggcggtagaa atcaccagcg ccggaatttg tgccaccaga ccgtcgccaa
     6001 tggtcaatag cgtataactt tccgccgcgt gtcccatgct catgccatgt tgcagcacgc
     6061 cgaccagcaa cccgccgaca acgttaatga ccatgatgag gatcccggcg atggcatcgc
     6121 cgcgaacaaa cttacttgcc ccgtccattg agccgtaaaa atcggcttcc tgagtgactt
     6181 cggagcggcg ttttttcgcc tcatcttcac caatcaatcc ggcgttaagg tcggcgtcaa
     6241 tcgccatctg cttacccggc ataccatcga gaacaaagcg cgcacccact tctgcgatac
     6301 gtccggcacc tttggtaatg accataaagt tgatgatcac gagaatgacg aacaccacga
     6361 taccgatagc gaaattgcca ccaacgagga agtgaccgaa cgcttcgacc accttccctg
     6421 ccgccgctgc gccggtatgc ccttccatca aaatgatacg ggttgaagcc acgttaagtg
     6481 ccagacgcaa cagcgtggta aacaacagaa tggtcggaaa cgcagcaaac tcaagcgtgc
     6541 gctgggtaaa catcgccacc agcaacacca tgatcgacaa ggcaatattg aaggtaaaca
     6601 acaggtcgag tatgaatgcg ggcagtggca gcaccatcat cgacaagatc aacaggatca
     6661 aaatcggtcc ggcaaggatc tgccattgtg tcgatttcag gtttgcgggc aggcgcagca
     6721 tcgcggccag attactcatg ggtcggtttc tcgttaataa aatccagggc ttccggcacc
     6781 ggaagatgag taggttgtac agggcgctgt ccaccagcca gacgccagcg tttcagttgc
     6841 cagacccagg ccagcacttc cgccaccgcg gcgtacagtt gacccgggat ttgttgacca
     6901 atctccgcat gtcgatacag cgctcgcgcc agcggcggcg cttcaagcgt cgggacgtta
     6961 ttttcagcgc cgatttcacg aatgcgcagc gcgaccagcc ctgcaccttt agcgaccact
     7021 ttcggtgcgc tcattttgtt ttcgtcatac tgcaacgcta ccgaatagtg tgtcgggtta
     7081 ttgacaatga catccgcttt cggcacatcg gccatcatcc gacgccgtgc ggcagctcgc
     7141 tgcatctgac ggatccgtcc tttaacatgg gggtcgcctt cgctttgttt gaactcatca
     7201 cgaatatcct gccgcgacat tcgcagcttt ttcaggtggc tgaagatttg gaaaaagacg
     7261 tcaaatccca ccattggaat gacaccaagc accaccagca gtgcgcatag ccctaccaga
     7321 tccatcgcat tacccatggc ggtaatcgga gactcggcca ttaagcgcat catctgcggc
     7381 cagtgatgcc agagaaaaaa ccccgccacg ctgccaacca ggatggtttt caaaattgct
     7441 ttaagtaact ccgcgccagt ctgtgccgag aacatccgtt taatgcccga tagcgggttg
     7501 agtttggaaa acttcggctg caaggatttg ccgctaaata ccagccctcc cagcatgacc
     7561 ggagaaatga gcgccaccag caccacgccg ctaatcagcg gcagcagcgc cagcatggct
     7621 tctctgatca gcagaataat ttgcccgagg atcagattcg ggtcattgat aatactgtga
     7681 tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga cacaccgcca
     7741 aaccagataa cactaacgcc caccagcaaa atcagcagtg aagtcagttc acgggaacgc
     7801 gggatttgcc cctcttcccg cgctttttca agtcggtgag gtgtgggggc ttctgttttg
     7861 tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagcgt aaatgatgcc
     7921 agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt tagggctttt
     7981 gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc cttatcagac
     8041 cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc aagactatcc
     8101 aacagatcgt ccacctgatc ctgactggct accacaccgg ctttgctggt atcgacctga
     8161 ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc cgggatgttt
     8221 tccaacagca ccatcagcaa ctgacgttcg atctcctgaa tgacatccat catccgctta
     8281 atgacctgcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag cagttgcgcg
     8341 ttagtaaagc tggtatgcgc gggtacatct gccagaaatt tccgcgtatc tgttaccagt
     8401 tcacgggcgt cggcaaggtc aatcggatcg gcaaaccagt catcccaacg ttgggttaac
     8461 gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac actgttcagc
     8521 gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc gcgcgcatcg
     8581 gggatggctt ccgccgcttc ggcaatggcc tgatccagtc ccagttcccg caaactgtcg
     8641 cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga atgctcgtca
     8701 gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt tctcaaagat
     8761 tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat agccactggc
     8821 ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca ccattaacac
     8881 tggcaatgct gacatcgcgc catccgcacg aatcgttttc agcaattcca ggccatccat
     8941 gttgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca acttattgag
     9001 agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt tcagcaggtt
     9061 acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa gttctttatc
     9121 cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat ttttgccaac
     9181 atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat attgatggcc
     9241 tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgccagggt ccatgccccc
     9301 gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt caggatcacc
     9361 ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc taccgaaggc
     9421 cgatgacggt taaccgccgg gccatcatga attttgattt ggtaatttgc gccactacgc
     9481 gccagctcca tatgccgatc gcccggcgca atataggcat gccccggcaa aacacgttcc
     9541 ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc ggcaaaagag
     9601 cgggtgaaac cgggcggcat atgctgggta attaacagtg ccgggctgga aagcggcaac
     9661 ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat cgcaatcagt
     9721 ttttcagaac tcaacaacgg ccctgccttc agcgttgtcg gtgccgacaa tggcttatgt
     9781 gctgcaaggc tcgcctttgc tgctgtacgc accttttcag caatcatttc gctgtacgcc
     9841 agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc ccccagctcc
     9901 agcgcgcgca gcgtgacttc tgatcctttg ccggtcaggg aagaaaccat cacaacgggc
     9961 attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg cggcatttca
    10021 acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc gaccagcgga
    10081 tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc tgtcatgatc
    10141 tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat cgttaatcct
    10201 tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg tggctaaagt
    10261 tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca aagcggcgca
    10321 aaatctcctg ctgggtagtt tgatcgaagt agatcatgac gttacgacag aagatcgcat
    10381 caaacggccc cggcacggtg tactgtttcg ccagtaaatt cagcggggca aaatcaacat
    10441 agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc cctcgcatga
    10501 aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga tagataccgc
    10561 ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc cagcgtccgg
    10621 gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct tcgccggtcg
    10681 aagccgccgc gctccatacg cgatactcgc cagaacggcg acatgcgtga tccgcgagca
    10741 gagggaaatg atgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc gaattgataa
    10801 acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa tgaccgaaat
    10861 ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg tcgcgtttat
    10921 ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc cgaaaatgcg
    10981 cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc ccacagggca
    11041 gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca atttgtaact
    11101 gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc acacgcgacg
    11161 aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct tctaccagcg
    11221 aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc tgctcaatcc
    11281 cccgccgttg ttcttccgac gccgaagcaa tttctcccat aatgtcgttc acgcgggtca
    11341 ccgaactgac aatatcggtc atggtcgcgg cggcgttatt caccagtttc gaaccctgct
    11401 ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc tgcgcgctac
    11461 ggctggcaag attgcgcact tcacctgcta ctaccgcaaa accacgcccc tgctctccgg
    11521 cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa gcaattccgt
    11581 cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc tgcatggtgt
    11641 gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca ttttttgcca
    11701 gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt aattgctcca
    11761 tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt gagagatcgt
    11821 tattgcctgc gacaatctcc gcgatcccaa tgtgcatctc atggcttccc ttacgcacat
    11881 cactcaccgt cccacgcaaa gcctgctgca tggtcttcag gctggcaaaa atggcggtga
    11941 tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca atgctgtcaa
    12001 aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac cacagcgcac
    12061 tgctgatgta gatcgccgca acaataatca tgctgataaa caccagcgcc gaaatctgat
    12121 agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc agctgccagg
    12181 cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg aggaaatccg
    12241 aaagttgatt actttcaagc caggccgcct gatgttcgag atcgttgtgc cagcgggcaa
    12301 aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca gtcatcgcca
    12361 taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc gtcatcaacg
    12421 ttttaatgtc atccgccgga tagctaagcg ctgtcagagt gcctgctttg ttcagcgcgg
    12481 tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc tgattgctct
    12541 gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac atgccgttac
    12601 tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc gaaattcgaa
    12661 tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta aatactcgtc
    12721 aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt gccggtggtt
    12781 gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg gctgccagac
    12841 gaaacgcgga aaccgcttgc gttaaacgac tagcctgttc ttccagcgca gctgcagcgg
    12901 cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt tccgaaaccg
    12961 ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca atctcgccca
    13021 taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc ccggcgcttt
    13081 cgaccagcac cgaaccggta tcaacgcgcg agacggagtc ttcaatgaga gctttgatct
    13141 cttttgccgc ctgggcgcta cgactggcaa gattacgcac ttcgcccgcc accacggcaa
    13201 aaccacggcc ctgttcaccc gctcgcgcgg cttcaaccgc ggcattcagc gcgaggatat
    13261 tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc ttcgaactat
    13321 cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg tgctgggcgg
    13381 tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg ttttgcttca
    13441 ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat gcctgctgtt
    13501 cagtacgcga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg gcatagatgg
    13561 catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc atatgtgaaa
    13621 tgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta ttcgccaggt
    13681 taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggtgta agcaacatac
    13741 ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg ataaccgcca
    13801 gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg cgatacagtt
    13861 tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc attccctggg
    13921 ttggctgagc gaaataagct ccagtattgc cataatcaag ataatcaatc agttcagtta
    13981 atgctgtgtg atagttttta tatttttcat caatattacg actggtagcg accatttcag
    14041 gtaacggtgc catacttttg aattttttat aatgagtcgc ggcctgggcc aatgttttcc
    14101 tggcgctatc gagcaattca actttggcat tactttgttg attggaggaa tccatcatca
    14161 ttcgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc caggttgacg
    14221 tcagctcgcc ctgctgttcc cgtaattgat tggaaaccac aaagctcttc tggctatggt
    14281 gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat acccccagca
    14341 ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt cctgaaaaca
    14401 agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca cccgccgcga
    14461 aattgaaata aaacccgatg cgcagatcat cgggttcatt tcaattgagg aaatcaggag
    14521 aattacgcca cttctgacgc cgcgctatct aacagcgcca tctcttcgct gttcagcagt
    14581 ttttcgatgt tcaccagaat caacatacgg tcgcccagtg cgcccagtcc agtgagatat
    14641 tctgttgaaa gagtcacggc aaactctggt gccggacgaa tttgctccgc cgtcaatgaa
    14701 agcacgtctg aaacgccgtc aaccacgatg ccgaccaccc gctgtccgag attcaggacg
    14761 ataactaccg tgttgtcgtt atagtccaca tccacctggc tgaacttaat tcgtaagtca
    14821 acaatcggca caataacgcc gcgcagattc gtgacgcctt tgataaacgc tggcgtgttc
    14881 gcaatccgtg ttacctgatc gtagccacgg atctcctgca ctttcaggat atcaataccg
    14941 tactcttcat caccaagggt aaataccaga aattcctggc ctgacggctc gctggccagc
    15001 tttgttacat tcgtcatacc ggtcatattg ttaccttttt actcattcag gcggcggtgt
    15061 tcgccatacg ttgttcgcgg tttatcgcct gcaaggcgga gacatcaaca atcagtgcca
    15121 cgctgccgtc gccaagaatg gtcgcagcag aaatgccggg gactttgcga tagttacttt
    15181 caaggttttt aactacaacc tgatgttgac caattaattg atccaccagc aaggcgtagc
    15241 ggcgaccgcc actttgtaag atcaccacaa ttccctgggt agcttcggtt ttcgcgcccg
    15301 cgacgttgaa cactttccac agttcgacga tgggcagata ttcaccccgc acttccagca
    15361 cccgctcgcc gccggccagt ggatggagat cggcttcacg gggttgcagt gattccataa
    15421 cagcattcag cggcagaatg aaaacttcat ccgcaacgcg tacggacatg ccgtcgagga
    15481 tggccagcgt cagcggcagt aaaatgcgga tcgtagtgcc agtaccctgc ttcgactgga
    15541 tttcgacatg accgcccatc tcctggatat tacgtttaac gacgtccatg ccgacgccgc
    15601 gcccggagac gtcggtgacc tgctctgccg tggagaagcc tggtgcaaat atcagcatcg
    15661 cgacttcgtc gtcgctcatg ttttcgctga cagtcaaacc ttgcgaggcc gcttttgcca
    15721 gaattcgctc acggtttagc cccgccccat cgtcggtcac ttcaatgcaa atgttgccgc
    15781 cctgatgttc ggcagacaga attaaatttc cgacgctgtt tttacctgcg gcgagccgtt
    15841 tttctggcag ttcaataccg tgatcgaggc tattgcgtac caggtgggtc agcgggtcga
    15901 taatgcgttc tatcaggctc ttgtcgagct cggtggaact gcccaccagc gtcagttcta
    15961 cctgcttgcc gagttttccc gccagatcac gcaccagccg gggatagcga ctaaagacat
    16021 attccatcgg catcatgcga atcgacatca ctgattcctg caaatcacgg gcgttacgtt
    16081 gtaactgccc catgctggta atcaaatcac catgattaac cgggtccagt tcgctggaac
    16141 gctgggcaag catggactgg gtgataacca gctcgccgac gaggttaatt aattgatcaa
    16201 ccttttctac cgctacacga atgctggtgg attcactgct gcgcgtcgtt ttttcccgct
    16261 ccacgcgacc ggttggcgct tgttcggctg ccagtttaag cactggtggg gtggatattt
    16321 ttggcgagac ttctactgtt tcaaaggtaa tctgatcggc ttcaatcaca aaacagagta
    16381 ccgctgtgat gtcatcttcg gcgatatcgc ccggtaatat tgccgagaga gaatccgccc
    16441 ctttcaccac gtcagttaac gttgtcagat gccccagctc ttcttccagc aggtcgactt
    16501 cgctggcctt caggcgcgaa aggataattc gtcgcggcaa ctgactgcga ctctgctcat
    16561 cttgcggttc acttttggca accacactta atcgggtcac tgcggatggc gtttcgcctt
    16621 tcgcttctaa tgccagttga cgcaaggcct ggcagatata atcgaagctg gcggcatccg
    16681 gctcttgcga ctgtttataa gcgtcgagct gttcttgcat gatgtccttc gtttccaaaa
    16741 acagattgat aatgtcggtg ttgagttgca tctcacctcg tctggcttca tcgagcaggt
    16801 tttccatcag atgcgtggtt tcctgcaaaa cgctgaagcc aaaagttcct gcccctcctt
    16861 tgatcgagtg ggcagcccga aagatggcat tcaattgttc ggcatctggc gcttccggct
    16921 gcaaaaccag cagatgctgc tccatatcag ccaacagttc gtccgcttca tcaaaaaatg
    16981 tctgataaaa atcgcttata tccatgctca cgctgtcacc tcggttcggc tgatggcatt
    17041 gtgggaacac tgacctgtgg tgcaacctca ggtttttcca gggcgcttac tggctcattc
    17101 tggctttcgg cgttttcatg caaaatggcc tgttcggctt gtttgttcag taccagcagg
    17161 ctgatgcgac ggttgacggc atcatcaggt ccgcgatcgc ttaagcgcat cgttgccgcc
    17221 atgccgacga cacgtaacac tttgccgcta tccaaccctc cgaccatcag ttcgcggcgg
    17281 gatgcattgg cccgatcggc agaaagctcc cagttgctat atcctttctc accgctggcg
    17341 taggggaaat catcggtatg acccgaaagg ctaatacggt tgggaatacc attcagaacg
    17401 ggcgcgatgg cgcgcagaat gtcgcgcata tagggttcga catcggcact gccggtttta
    17461 aacatcgggc gattctggct atcgatgatc tgaatacgta gaccttcctg gaccagatcg
    17521 attttgagat gtggacgtaa cgcccgcagt ttcggatcgg actctatcaa ctggtcgaga
    17581 tcaccccgca atttccgcaa tcgactttgc tccatgcgtt ttttcaactc ttcgatgttc
    17641 ggctgcttat tcacttcccc ctggctttgg gtgtaatcat caccaccgcc gggaattggg
    17701 ctttcactat tagaaatgcg atcgccgccc gtaaccgctg tcgctagtgg agtccggaag
    17761 tactccgcaa tctgaatcaa ctcttttggg ctggagatgg agatcagcca catcaccaga
    17821 aaaaaggcca tcatcgcagt cataaagtcg gcataagcaa tcttccagga tccatgtgct
    17881 gccccgtggc ttttggcttt gcgtcgtttg acgacaataa tcggatgcgc ttgattcttc
    17941 atgcttcctc ggttgtcgtc tgttgttgcg gatttttcac cgcacgcaca tgctcttcca
    18001 gttcaataaa cgacggacgt tcgctggaat agagcgtttt gcgaccaaac tcaacggcga
    18061 taggcggtgc gtaaccgttc agattagaaa gcagtgtgac tttgacgcac tgcatcattt
    18121 tgctggtttc ggcgcttttc tgacgtaaaa cactcgctaa tggggaaata aatccgtaag
    18181 ccaataaaat gccgaggaaa gtccccacca tcgcatgtgc gataagcgcc cccagctcgg
    18241 cggcaggacg atcggctgaa cctaacgcgt gaacgacccc cattacagcc gcgacaatac
    18301 caaacgccgg aagtgagtcc ccgaccagcg ccagactgtt cgccgggact tctgcctcgc
    18361 tttcgtgcgt ctcaatctct tcatccatca gggcttcgat ttcgaaggtg ttcatgtgac
    18421 cgctgataat caggcgcaga taatcgacga taaaatcaag catgacgcta tccgcgagga
    18481 tgcgtgggta gctggcgaag atctcgctct cacggggatt ttcaatatca cgttccagtg
    18541 aaaacatccc catctgccgc gatttcgcca tcaaccgata aagcagagcc agcagatcca
    18601 tatacattgc tttggtgtat ttggagcgac gaaacagcaa cggcagcgct ttcagcgtgc
    18661 ctttaatcgc tttgccatta ttgccgacga taaacgaccc aatccctgca ccggcaataa
    18721 tcaccagttc agcgggttga tagagtgctc caaggcttcc accggtcatc aaataaccgc
    18781 cgaaaactgt accgagaaca accaggtaac ctaataagat aagcacgaca tcatccttcc
    18841 actgttgacc atgacaggat gttcagtcgt caggcgttaa cgcgcgattg gggcaaaaaa
    18901 aagcagcggt acgtcgttac cgctgctgga atgttgcgcc acatcgtatc agttaaacag
    18961 cctgtactct ctgttcatcc agcagttgtg ggataatatc ggcaggattc tgggaaagtt
    19021 tacgtctttt tactgcccgg gatggcggtt gacataagct gcaggcaaag ctgccaacag
    19081 gctggtgagc gtgggtaata aaattgccgc cgcagcagtt gcagctggaa agttgcagta
    19141 atccactttc aacaaaccgc accaatgtcc aggcacgggt taatgccagc agcggtcctt
    19201 cttctgcttg cgggcactgt tcaaggtata aacggtaggc tttgatcacc gcatcgacgc
    19261 cattacacaa accggttttc agtaaaaact gccatgcatt acagaacatc gaagcatgaa
    19321 cgttttgttc ccaggtcata aaccagtcgg ttgagaatgg cagcatgcct ttcggcggtg
    19381 ggcttccgcg cagttcttta taaagtttta tcaggcgtcc gcgacttaac tgtgtttcgc
    19441 tttccagcat ctgcaaacga gcgcccaggg tgatcaattc cattgccagc tgaatatccc
    19501 gcgcttcctg aacaatgctt ttttcactca tgatcaggcc cttttcttgc gcagcgcttc
    19561 ttcaggctga ttaacatcat tcagcaagcg tgttgagagc atgatgccgg tatgaatttg
    19621 ctggagatcg tcaacgcggg aatcttgcgt caactgagta atcgtctggt ggctgtcaaa
    19681 acggaagtga caaaccagct gattggtttc tgccagctta accatttgcg gaagagtcag
    19741 tgccgctaat gttgtcgcca tttcttcatt tatgccgaga cgaaacatag cggacgcttt
    19801 gtcctgaaca atcagacgct gtgcaagtag taaatatgac aagttgatgt cataaatgtg
    19861 tttcagcaac tcggaggtat gcattattcc cacccagaat aaccaacttt atttttatgc
    19921 ggtttcaccg caccccgtga tgtcgccggg aagccccggt aaaaaataat tagcattaga
    19981 atagttgcga taagctgcaa taagcagagc cacctttttg gtttaataag tccttacaaa
    20041 tagaaggggg tctttacact tatctaagat ttttcctaaa tcgacgcaac tgtactcgtc
    20101 actacacgca catacaacgg aggggggctg cgattttcaa taatgtgtga tgaagatcac
    20161 acaaaacact caattactta acataaatgt ataagtcata cttttgtttt gggtgtattt
    20221 caatctgtta aaaagttttt cgctacgcta gcaagcaaaa atgaaacagg aataatcgaa
    20281 atgggatgtt gcgcacagtt aaaataactc accgtaaata atcatctgct ataaataatc
    20341 actttcatgc aataccagat aagctatttt taaacagtca cttaccgcac aacaaactaa
    20401 ttaaatacaa agaaaggcaa caatcaactt aatgttaata aaatgtt
//
LOCUS       DAZKHC020000075        19701 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZKHC020000075 DAZKHC020000000
VERSION     DAZKHC020000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.075"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(110..1597)
                     /gene="yeeO"
                     /locus_tag="ACHOSM_RS16530"
                     /old_locus_tag="pgaptmp_003306"
     CDS             complement(110..1597)
                     /gene="yeeO"
                     /locus_tag="ACHOSM_RS16530"
                     /old_locus_tag="pgaptmp_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HGU4559766.1"
                     /translation="MWFHLLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLAESFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            1758..1833
                     /locus_tag="ACHOSM_RS16535"
                     /old_locus_tag="pgaptmp_003307"
     tRNA            1758..1833
                     /locus_tag="ACHOSM_RS16535"
                     /old_locus_tag="pgaptmp_003307"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1791..1793,aa:Asn,seq:gtt)
     gene            complement(1871..2821)
                     /gene="cbl"
                     /locus_tag="ACHOSM_RS16540"
                     /old_locus_tag="pgaptmp_003308"
     CDS             complement(1871..2821)
                     /gene="cbl"
                     /locus_tag="ACHOSM_RS16540"
                     /old_locus_tag="pgaptmp_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HGU4559767.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2923..3840)
                     /gene="nac"
                     /locus_tag="ACHOSM_RS16545"
                     /old_locus_tag="pgaptmp_003309"
     CDS             complement(2923..3840)
                     /gene="nac"
                     /locus_tag="ACHOSM_RS16545"
                     /old_locus_tag="pgaptmp_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HGU4559768.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVMSSPVMEKRQWQLVS"
     gene            4167..4242
                     /locus_tag="ACHOSM_RS16550"
                     /old_locus_tag="pgaptmp_003310"
     tRNA            4167..4242
                     /locus_tag="ACHOSM_RS16550"
                     /old_locus_tag="pgaptmp_003310"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4200..4202,aa:Asn,seq:gtt)
     gene            complement(4297..5232)
                     /gene="ldtA"
                     /locus_tag="ACHOSM_RS16555"
                     /old_locus_tag="pgaptmp_003311"
     CDS             complement(4297..5232)
                     /gene="ldtA"
                     /locus_tag="ACHOSM_RS16555"
                     /old_locus_tag="pgaptmp_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4559769.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5294..6373)
                     /gene="cobT"
                     /locus_tag="ACHOSM_RS16560"
                     /old_locus_tag="pgaptmp_003312"
     CDS             complement(5294..6373)
                     /gene="cobT"
                     /locus_tag="ACHOSM_RS16560"
                     /old_locus_tag="pgaptmp_003312"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HGU4559770.1"
                     /translation="MQILADLLNTIPAIDSAAMSRAQRHIDGLLKPIGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AEQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     QELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFISYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6385..7128)
                     /gene="cobS"
                     /locus_tag="ACHOSM_RS16565"
                     /old_locus_tag="pgaptmp_003313"
     CDS             complement(6385..7128)
                     /gene="cobS"
                     /locus_tag="ACHOSM_RS16565"
                     /old_locus_tag="pgaptmp_003313"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HGU4559771.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGESILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKLTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7125..7670)
                     /gene="cobU"
                     /locus_tag="ACHOSM_RS16570"
                     /old_locus_tag="pgaptmp_003314"
     CDS             complement(7125..7670)
                     /gene="cobU"
                     /locus_tag="ACHOSM_RS16570"
                     /old_locus_tag="pgaptmp_003314"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HGU4559772.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7822..7995)
                     /locus_tag="ACHOSM_RS16575"
                     /old_locus_tag="pgaptmp_003315"
                     /pseudo
     CDS             complement(7822..7995)
                     /locus_tag="ACHOSM_RS16575"
                     /old_locus_tag="pgaptmp_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(7974..8162)
                     /locus_tag="ACHOSM_RS16580"
                     /old_locus_tag="pgaptmp_003316"
                     /pseudo
     CDS             complement(7974..8162)
                     /locus_tag="ACHOSM_RS16580"
                     /old_locus_tag="pgaptmp_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            9342..10676
                     /locus_tag="ACHOSM_RS16585"
                     /old_locus_tag="pgaptmp_003317"
                     /pseudo
     CDS             9342..10676
                     /locus_tag="ACHOSM_RS16585"
                     /old_locus_tag="pgaptmp_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(10715..11389)
                     /locus_tag="ACHOSM_RS16590"
                     /old_locus_tag="pgaptmp_003318"
                     /pseudo
     CDS             complement(10715..11389)
                     /locus_tag="ACHOSM_RS16590"
                     /old_locus_tag="pgaptmp_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc48 family transposase"
     gene            complement(11329..11484)
                     /locus_tag="ACHOSM_RS16595"
                     /old_locus_tag="pgaptmp_003319"
                     /pseudo
     CDS             complement(11329..11484)
                     /locus_tag="ACHOSM_RS16595"
                     /old_locus_tag="pgaptmp_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063945310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            11535..11720
                     /locus_tag="ACHOSM_RS16600"
                     /old_locus_tag="pgaptmp_003320"
                     /pseudo
     CDS             11535..11720
                     /locus_tag="ACHOSM_RS16600"
                     /old_locus_tag="pgaptmp_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280452.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
     gene            complement(11821..12150)
                     /gene="tmaR"
                     /locus_tag="ACHOSM_RS16605"
                     /old_locus_tag="pgaptmp_003321"
     CDS             complement(11821..12150)
                     /gene="tmaR"
                     /locus_tag="ACHOSM_RS16605"
                     /old_locus_tag="pgaptmp_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HGU4559773.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(12322..13380)
                     /gene="yeeA"
                     /locus_tag="ACHOSM_RS16610"
                     /old_locus_tag="pgaptmp_003322"
     CDS             complement(12322..13380)
                     /gene="yeeA"
                     /locus_tag="ACHOSM_RS16610"
                     /old_locus_tag="pgaptmp_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU4559774.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(13579..14052)
                     /gene="sbmC"
                     /locus_tag="ACHOSM_RS16615"
                     /old_locus_tag="pgaptmp_003323"
     CDS             complement(13579..14052)
                     /gene="sbmC"
                     /locus_tag="ACHOSM_RS16615"
                     /old_locus_tag="pgaptmp_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HGU4559775.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNRLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(14171..15337)
                     /gene="dacD"
                     /locus_tag="ACHOSM_RS16620"
                     /old_locus_tag="pgaptmp_003324"
     CDS             complement(14171..15337)
                     /gene="dacD"
                     /locus_tag="ACHOSM_RS16620"
                     /old_locus_tag="pgaptmp_003324"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HGU4559776.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPQIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            complement(15972..17927)
                     /locus_tag="ACHOSM_RS16625"
                     /old_locus_tag="pgaptmp_003325"
     CDS             complement(15972..17927)
                     /locus_tag="ACHOSM_RS16625"
                     /old_locus_tag="pgaptmp_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-binding domain-containing protein"
                     /protein_id="HGU4559777.1"
                     /translation="MSDITANVVVSMPSQLFTMARSFKAVANGEIYIGKIDADPVNPE
                     NQIQVYVENEDGSHVPVAQPIIINAGGYPVYNGQIAKFVTVQGHSMAVYDAYGVQQFY
                     FPNVLKYDPDQLRQELSAPGGVNLVNGAAKKEDTDKIQAQNAAFAYIEDYESLVEEDD
                     WTAAINAAFSTGKPVIGSGTYKVKGIIESKGQEIIGGFKIDTSRHSLGVVNVSSTDKL
                     PVDDKLRIMYVESAYDLSELAFIKSLGINTIKHYGNFAASPDDSAGSVQKVLDNAATL
                     GMRVIASTENGESGLTTVQFLTKYRYHPALLGWATFDEAMSRGFSYADQKDRYDLIRQ
                     YSNKPIAIVDAWDGADVISDLLLEYYDIVLADPYAVRQSTGTIDQRVANDLNRMRRAF
                     SAMVAHSRQKKVIPVLGTFTSSSGAGTDDVTQILRGAEVFRNAGNGEFAVFVWDGAGD
                     KTITGGVRSNAYFRSFVAATAEIKYSVPYKTEVFLFGGNHIIGHKPLNDIIDRVVNSD
                     GFSSDTFHGGKAYPIHVISGSEISDRTTTTEGWNKSGIGFKGTLAMLVTNIPFRKNIV
                     VFGEYSAITPDIGNVNGNFTVFGSYDGGYTTIQRSDQVVTGTSVLIEQYATTPNSNER
                     LCIRTSAVIDSNYMRRIFSGLIASTDW"
     gene            18049..18315
                     /locus_tag="ACHOSM_RS16630"
                     /old_locus_tag="pgaptmp_003326"
     CDS             18049..18315
                     /locus_tag="ACHOSM_RS16630"
                     /old_locus_tag="pgaptmp_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HGU4559778.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSEMVKKIVFDTLKTMYSNNKKET"
     gene            18386..18871
                     /locus_tag="ACHOSM_RS16635"
                     /old_locus_tag="pgaptmp_003327"
     CDS             18386..18871
                     /locus_tag="ACHOSM_RS16635"
                     /old_locus_tag="pgaptmp_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559779.1"
                     /translation="MKKSLLIIPLLLVGCAKVSDYQASCEQRYQKLSDMANCLDASVK
                     NDSRMASAPTPKLYVLAAKMLGQGVDEGKISDAQARLELQNLYVQLQSQEQAQQIAQS
                     QAFQQALLNYQAVNTMQAIEQKARQPVITQPYPTRVDTYTNCNSGFGNTVTCNSSSNI
                     R"
     gene            complement(18962..>19701)
                     /locus_tag="ACHOSM_RS16640"
                     /old_locus_tag="pgaptmp_003328"
     CDS             complement(18962..>19701)
                     /locus_tag="ACHOSM_RS16640"
                     /old_locus_tag="pgaptmp_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HGU4559780.1"
                     /translation="GERPVLPNRSDAAIQRVYKAMTDDINGAIGQNLGNDTLRKYQQA
                     NAVYADEAAKLKNTRLKNVLMKGDLTPEVVNNMLFSKNKSEIKTLYNSVGRVGRAQMR
                     NGIIGKAMEKSGGSPDQFLRQLNILQNQTGITFKGQDAAYLKGLKNYLQSTQQAAKAA
                     VTTPTGQQTIPFIIGYGTAMNPATTGAAVSYGLLTRAYESEPFRNAMLRMANTPRGST
                     AFEKAMQQAQKAINALTQGAKSDSLSE"
BASE COUNT         5402 a         5015 c         4266 g         5018 t
ORIGIN      
        1 tacagaggaa tcgtgagaac gaggcgaata ttagcgatgc ccacccacaa tgtcaaagcc
       61 tgttttttaa atttgaaatc gtttgctgaa ataatctgca ttttgtcgtt tattccgaca
      121 caactggctt tttttcacac ttttgcggct cgggtcgagg gtatttccat agccaacgtc
      181 cagtaaccat tcgccagtaa aacagcacgg cccgcacagc ccagtcggca aacattccca
      241 tccagacacc aaccacaccc cagccaagca tgatgcccag cacataaccg acgacaaccc
      301 ggcaacccca catgctcaac atagaaaccc acatagcgta acgggcgtca cgagccccct
      361 taaatccagc gggtagcacc catgaggcgg accaaatagg cataaataaa gcatttagcc
      421 aaatcagaat cacaacgaca tgtttaacct gtggatcctg ggtgtaaaac gatgccataa
      481 ccccggcaaa gggagccgtt agccaggcga tggccgttaa tccaagagtg gacagccaga
      541 acacatgccg caactgaatc tctgcttgcg ctatctgccc tacccccaac cttcggcctg
      601 taatgatcgt agaagcagag ccgagcgcac ttccgggtaa gttgataaga gccgcaattg
      661 aaaacgcgat aaaatttccg gcaataacac tggtccccat cccggcaacg aacatttggg
      721 ttaataaccg accactggta aataacactg attcgacact cgctggaata ccaatcccca
      781 tgacttccca gataatgcta aaattcagcg gtttaaaata gctctttaac gaaatcctta
      841 gcgcaggatt aaaaccaatc gccagcaccc acaaaattgc aactgcgcca atataacgag
      901 aaatggttaa acccaaccca gccccgacaa atcccagtcc cggccaggag aaaaggccgt
      961 aaatcaatat gccgctaata ataatattaa gaatattcag gctaccgtta atcagtagcg
     1021 gtattttcgt attccctgca ccacgaagtg ccccgctacc aatcaaagtg atggcagctg
     1081 ctggataact gagtaccgtc agctccagat aagtcaacgc cagtgcttta acttctgtcg
     1141 tggcatcacc cgcgacgaaa tcaataattt gctcgccaaa atgatgaata agcgttgcca
     1201 acagtacggc aaacaacgtc atgatcacca atgactgccg cgtcgccacc ctcgctcgtc
     1261 gtcgatcccg cttaccgaga ctaaatgcca caacgacagt agtaccaaga tcgatagcag
     1321 caaaaaaagc cataatgacc atattgaagc tttccgccaa tcccacgccg gccatcgcat
     1381 cttttcccag ccagctgacc agaaaagtgc tcagaacccc catcaacagg acacaggcat
     1441 tctccatgaa gataggaaca gcaagcgggg ttatctcgcg ccagaacaac actttgtagc
     1501 tcttgcgttt agcgtgccag cgagtgccgt gaacaacctg gcgtaaagca gaggagatat
     1561 tcaaagccga ccttaattgc agcaagtgaa accacatttc aaataatgag ggagaatcag
     1621 caaagctgca aagattttcg ccaacaaatt gtctgcaaat gcaacaaact gttgatagaa
     1681 acggcaaaca gttgggaaaa ttaaaaatcg ggtttgacaa aagatttttc gccgttaaga
     1741 tgtgcctcaa cagcgattcc tctgtagttc agtcggtaga acggcggact gttaatccgt
     1801 atgtcactgg ttcgagtcca gtcagaggag ccaaattcct gaaaagtccg cttttatagc
     1861 gggctttttg ctatatctga taatcaattt cctcttcact gttctccatc acttggcgct
     1921 tgatatcctc aactgacagt cctgcattac aaagttccag aaagcgccag acatagttac
     1981 gttgaagttg ccctcgcttc agtcccaacc atacagtatt ggcatcgaaa agatgccgcg
     2041 tatccagacg gattaaattc tcttcctctt gttcgccgct ggattgttcg gcaactaatc
     2101 caatccccag cccaagagca acataggttt taatgacatc agaatcctgc gcacttaata
     2161 caatatctgc cagcaaacct ttgcgggcaa atgcgtcatc aatacgtgag cgccccgtaa
     2221 tcccctgtcg gtaagtgatt aacggccact tcgctattga ttccagcgtc aacggtgtaa
     2281 tttgcgtcaa gggatgatcg agtggaacaa gcaaactatg gtgccaacga aaccacggga
     2341 aggcgacgag ctgcgggtca ttactcaaac gctcgctggc gataccaata tcagcttcgc
     2401 cattttgcaa caacgtcgca atttcctgtg gcgtaccctg gattagctcc agccgaacct
     2461 ccgggaaaag ttcgcgaaaa gctttaatga cctcaggcaa gctataacgt gcctgagtat
     2521 gcgttgttgc aatagtgaga acgccagacg tatcgttggt aaacaggtct gcaagccgac
     2581 gaacattact ggcttcattc agaatacgtt ctgcaatgac cagtaatgct ttgcccggtt
     2641 cagtcatgcc cagcagtcgc ttacctcgtc gaacaaatat ttcgatgcca agttcatcct
     2701 ccagttcccg aatatgacgg ctgacgccgg actgtgaggt aaaaagcata ttcgcaacct
     2761 ctgtcaggtt gtaatcctga cgtgcagcct cgcggattat ctttagttgt tggaaattca
     2821 cggtaaactc cgggcagttc agatcttccg ttattgttaa agtctaatgc ccggcataac
     2881 aaataataaa aacccgcatc ttattccatc ccgatataac gcttagctca ccaattgcca
     2941 ctgccttttt tccatcactg gagaactcat cactgacatt aacaactctt tcactgcctg
     3001 tgcctgtggt gataagttcg ctctggcggg taaatttaat gacaaagaga gactcatgga
     3061 aggtgtggta atgcgtgaca tccacccgtt tactgcgcca cataacgaac gcgcggccga
     3121 ttcgggtaat actgcaacgc ccatgccgct ggcaatcgct gcggtaagcg tggcaataga
     3181 ctcaatttca ccaatgactt ttgccgtgag tcgccgtagg gaaaaagcct catcaacacg
     3241 aagtctaaca gcactgtaat cactgggaag aaagaggttc atttgcgcaa tagcattaac
     3301 atcaacgctt tgccccggac aatcttgagt ccctaccagg aaaagatctt ctttcagtaa
     3361 agcctgactg gatacaccag ccacagggga atgctcataa atcaccgcca tatcgagttg
     3421 gtgatttatc agtttttcgt taagcactgc accactattt tcatgaagat agataacgac
     3481 ctccgggaat tcagcgcgaa ccgcctgtaa taagggcatg gtgatggatg acgcagcggt
     3541 tcctggcgca aagccaatcg agacttgccc cgataatgac tgaccaacgt tatgcaccgc
     3601 cagttgggcc tgctcacact gacgtaaaat ggcccgtgca tgggtataga gaatttttcc
     3661 ggcgtctgtt ggcgtaacgc cccgttttgt gcgaatcaaa agctgttgat ttaactcacc
     3721 ttccagtgtg gcaacctgct ggctgagcgc tggttgtgcg atatgcaata cttcagcagc
     3781 ctgggtcagg ctaccaatat ctacaatttt tacgaagtat ttcaggcgtc tgaagttcat
     3841 gttgcctccg gtttttaaga atcggcccaa gtgccgccat tacttacaac cagaatgcaa
     3901 gatgcctgcc agttctattt tggcgttgat gcgcaagcta accaactgtc aaataagaga
     3961 ttatgataga ttcgtcattg gcccttttta tcagcagtcg cattcacctg ccctataaaa
     4021 ggagggtatg caccacgatg gttcattacc caataagatt gaaagctcac cactttgttg
     4081 aaattggcag caaacaaaca aaaaaatgca tttcaccctt tgacatcacc atgcactgcc
     4141 attaatatgc gccccgttca cacgattcct ctgtagttca gtcggtagaa cggcggactg
     4201 ttaatccgta tgtcactggt tcgagtccag tcagaggagc caaattcaaa aaagcctgct
     4261 ttcgagcagg ctttttactt tcaattacca acgctcttaa aacatctgtc ttgaaccaga
     4321 actaatttgc acaggcattc ccgatcgatg ttgcaacgca gcatttgcgc gatttacatc
     4381 aacttcttgc ccgttgataa acgcccgcaa agatggggtt accggcaatg gcacttttcg
     4441 gtcagactca tattctgcac gattgcgcga cagcggctca tgaacttcca gccactttga
     4501 accatccggt tccgttgtgt atttcactgg ctgatcaata atttgtacac gagtgcctac
     4561 aggaacatta tcaaacagat atttgatatc gtcattgcgc agacgaatac agccctgact
     4621 tacccggagt ccaataccaa aattggcatt ggtaccgtgg atggcataca acctgccaat
     4681 ataaatcgcg tacagcccca tgggattatc gggacccgca ggaacaaatg cgggcaaact
     4741 ctcccctcgt ttcgcatatt cgcgccgagt gttcggcgtt ggcgtccagg ttggagcttc
     4801 ttgtttacgt tcaacggtag tcacccagtt acgcggggtt tctcgcccag cctggccgat
     4861 accaatagga aagacttcca ctgtattact gtctggcgga tagtaataaa gacgcatttc
     4921 agcgacgtta accacaatcc ctttacgaac agtggcgggc aaaatcagtt gctgcggaat
     4981 ggtgagttgt gagccaggct tcggcaaaaa aacatcagcg cccggattcg cttccagcat
     5041 attacttaac ccttgcccgt attgtgcggc aaaagtctcc agcggctgag tattgtgatc
     5101 aggaacagtt acagtaagcg actgccccac taaacggcta ccctctggag gtaatggata
     5161 agttaccgcc aggctatttt ggctggcaaa aagcagagca aatgagcaaa gaatatttac
     5221 acgacgcatc atgtcccttt cctaagtcgc gaatactatc cgataagtat agcttttatc
     5281 agacttttcg tttttagctg ttcaaatcag aagtcgtatt ccccggtaga acaatattac
     5341 tggcagcaag ttcgcccatg ttgttgtata tcgcacaagc agcttcgatg atggacatcg
     5401 ccagagctgc accactcccc tcacctaaac gcatatccat attgagataa ggctccagcc
     5461 ccaaatgcga gagcgctata cgcgcgcctt tttctgccga caagtgagaa ggaatgagat
     5521 acggtttgat tgcaggagac atctggcagg ctgcgagcgc agcagcataa gaaataaatc
     5581 catccagcaa cacgggtaaa ccgcaggaag cagcacctaa catcactcca gctattccga
     5641 ccaaatcaaa tccacccact tttgccagga catcaacacc atcctgagga tttggttgat
     5701 tcaacgtaat cgcccgacgc acaacatcaa ttttattagc cagtttatct gtcggcaggt
     5761 ttgcgccaat cccaaccact tcttcaggat cccggccagt gattgtgctg actattgctg
     5821 ctgccggtgt cgtgtttgcc atccccagtt cacctacacc aaacagcgtg acaccgtttt
     5881 ttgccagctc ctgcgtataa catatgacgt ccaaaagcaa cttttcagcc tgacggcgac
     5941 tcattgccgg agctgaagca atattgccgc tacctcgtgc gacacgcatg ttgataagcc
     6001 cggggatagg ctcagcagta tcaataccaa catcaattac gtggacgtta gcgcccgctt
     6061 gttctgccag cacacacacg ccggttgttc cacgggtcat attttcagcc tgtatggctg
     6121 tcacttcttt tggagaaata gcgacccctt cctcccagac gccgtgatcg gcacacataa
     6181 ccagtaccgc ttttttgccc acatgcggta tgccattcaa ccccggcatt cctgccagtt
     6241 gtatggcaag cgcctccagc tttcccaggc taccaatagg tttgagtaac ccgtcaatat
     6301 gccgttgtgc acgcgacata gcggcagaat cgatggctgg gatcgtattc agtaaatcgg
     6361 caagtatttg catgtcacgt ctctttatag cagtgccagc aggaagacca gttcaccaag
     6421 ctctatggct gctcccagcg tatcgcccgt ttgcccgccc agcgtaagtt tgagaagctg
     6481 accgaggatg aaaatcgcca ccatcgtcac caccatagca gccacaccat gcatacccgg
     6541 caataatacg gcagcaaaaa tagcggctaa gccgagtgtg acgcaggttt gtcgcccatc
     6601 aattttgcca atgaacacat tgccaagccc ttcttcccgc gcataacgat gacgatacat
     6661 caataatgcg gcagtcccgc ggctgaccgc acatgctgcc gccagcgatg caaggatcga
     6721 ttcgccacgc agagccaact cgcttaacac cagaatcttt gccagtacca caaaaatcaa
     6781 tgccagaccg ccgtgggtgc ctaaacggct atcacgcatg atttccaaca tgcgatcgcg
     6841 gctacgtgca gaaaatacgc catcacaggt atcagcgagg ccgtccagat ggaatccccc
     6901 ggtcatcagc accagcacaa gtacgctaaa cagtgccgcc agtggtgcgc cacaccatgc
     6961 ctgcagcacc atgaagacta gcccgctaat cgcgccaagc aataatccaa tcaaaggaaa
     7021 agtaataata ccgcgagaat aatgctcgaa atccagtccc tgggaccagc gacgcggtac
     7081 aggcagacgc gtaataaatg agagcatcgc ccaaaataat ttactcattt gatttttact
     7141 ccaatacccg aaaccaccag ccatacttca tttgccgcag cggccaactg ctgatttacc
     7201 cgcccggcaa tatcacgaaa atgtcgtgcc agacgactct ccggcacaat ccccattccc
     7261 acttcgttag tcactaatac aacctttgcg gggcaacgtt ggcaggcagc aatcaacgac
     7321 tgaatctcag cattaatcgc ctgttccatc gcctgataat cccattcatc agggtcttta
     7381 tcgccgccat aatcaaacaa cagattagtc accattgtgg taacgcattc cagcaacaca
     7441 acctcattcg ggttaatgtc tgcatgaatt aattcatcaa gatgttgcca gcgctccact
     7501 gtgcgccagt gctccgggcg gccttgccga tgatgttcta tccgtgcagc catctcatca
     7561 tcaaggattt gcgaggtagc gatatacaga acctgtgaag agtccccaat aagcgcctct
     7621 gcgtggcgac tcttcccgct ccgtgcgccc cccgtcacca aaatcatcat accgactcct
     7681 gattcaaatg tatgatggta ttcattgaac agaacaatgg atcccccacc gttgctgacc
     7741 gcaaaacatt tacgcgatta atactgcgcg taatataatt aaaaatccag cattctcaat
     7801 ccattacgaa ataagatggc attacggaat agccagactt tctcgtgtta acgtccgaca
     7861 aaatgcgtca ttgacagcgg tttctgtgga ttgacacatt tcattcagat aatgaattaa
     7921 tgctactgcc ggaccaacca gcaaacgggc attgtgttct gaaatccagg ctattgattc
     7981 aacgtcagcg acagatctgc gctgtaaaag aacttgtaaa acaacgcaat agaattatcc
     8041 ggaatggtgg cgactatgca ctagaacacc acaatttcgc tctctcggta aggcactgtc
     8101 acagcattca acagaatgtg acttgccaga tttattagcg ccaccagatg tttaaccggg
     8161 taaccacgac ccagtcgggg atatattcca tcttctcgct gacatcatca agaataaaaa
     8221 ggtttatcac actcagagca acgttcttca gcaggcggga taattcttca acatttagaa
     8281 gatgtgttat taaaggcata caaactggac gtattgtatt ttcttttggt ggttgaccta
     8341 aggtagcagt ttatcctgat gcgctgagat ttctgtaata tctacgtcaa cattctcctc
     8401 gaatagaaaa tgcagccccg gcaagtgatt cacatttttt aacaaaacgt tgttagcgtg
     8461 ccattttcag aagctctcca agaaagcgaa ttcttttctc aaattctgca ttctcagaca
     8521 cagctttcta ctccatggca atggcccaaa tacgaagttg ctcaggatca ttagtggtgc
     8581 tgagaagtta aataaccatg cggagtacca ctcgtcatca cgtatttata tatcatgaag
     8641 ctattgttgt gttttgtaca tcagtagata tattctgata tactcctttt gctagacata
     8701 acctttcacc tgcttgcaaa gcttctgtgt tctgacattg ccaaattgtt gcaattctgt
     8761 atccagcctt ctttcagtca tagcttcggg ccgcgataag actcactgat ctgaccctga
     8821 ttcctcttgc agactttata gaccaattaa aatgcagttt ctgcaggtca acgtctgacc
     8881 atcattgtca tcactctggc cattagagta accttctgca ttcatccttt tgtaaaaagt
     8941 ttatatcagt atcagcaatt aaccggacct gatactgata tgagtcttac cgcatatatg
     9001 gttaatttca gcaattaatt acattatcca cgccaaagta tttgtcatca caatgatggt
     9061 atcttctttc agacaccatt ttttcaactc cgttttccac ggaccgcact cttatgtcaa
     9121 gagtgcggtc cgtggataca accagagacc gactgacacg agtcagagga aacgacggat
     9181 atgttcagtc gtaaaatatc tatcaaaaaa catgattaag gtcaaaaatg tttgatattt
     9241 acaatttatg aagatgacaa taattataga cacatgagaa cataaatgaa aataattatc
     9301 attacagcaa tcatttgtac tttgtattga tgagggatga aatgttatat aatatacctt
     9361 gtcgaatttg tatcctttcc actctgtcat tatgcatttc tgggatagtt tctactgcaa
     9421 ccgcaacttc ttcagaaaca aaaatcagca acgaagagac gctcgtcgtg accacgaatc
     9481 gttcggcaag caacctttgg gaaagcccgg cgactataca ggttattgac caacaaacat
     9541 tgcagaactc caccaatgcc tccatagccg ataatttgca ggacatcccc ggggtagaga
     9601 taacagacaa ctccttggca ggccgtaaac aaatccgcat tcgtggcgaa gcatcctccc
     9661 gtgttttaat tctcattgat ggtcaggagg taacttatca gcgcgccgga gataattatg
     9721 gtgtgggact gttgatagat gagtctgcgc tggagcgtgt tgaggtagtg aaaggtccat
     9781 attccgtact gtacggttca caggcaattg gcggtattgt taacttcatc accaaaaagg
     9841 gaggtgacaa acttgcatct ggagttgtga aagctgttta taattccgca acagcaggct
     9901 gggaagaatc aatcgcggtc caggggagca tcggtggatt tgattatcgc atcaacggta
     9961 gttattctga tcagggcaat cgcgatacgc cggatggacg tctgccgaat accaactatc
    10021 gtaacaatag tcagggtgta tggttgggtt ataactccgg aaaccatcgt tttggcctct
    10081 cgcttgatcg ctacagactc gcgacacaaa cttactatga ggatccagac ggaagttatg
    10141 aggcatttag tgtcaaaata cctaaacttg aacgagagaa agttggggta ttctatgaca
    10201 cagacgtgga cggtgactat ctaaaaaaaa ttcatttcga cgcgtatgag cagaccatcc
    10261 agcgccaatt tgccaacgaa gtaaaaacga cacagcctgt tcccagtccg atgattcagg
    10321 ctctgaccgt tcataacaag actgacaccc atgataagca atacactcag gcggtcacat
    10381 tgcagagtca cttttcgctg cctgctaata atgaacttgt taccggtgca cagtacaaac
    10441 aagacagggt cagccaaagg tccggtggca tgacctcaag caaatctctg accggcttca
    10501 ttaataagga aacacgaact cgctcctatt atgagtcaga gcaaagtaca gtctcactat
    10561 tcgcacaaaa tgactggcaa ttcgccgatc actggacatg gacaatggga gttcgccaat
    10621 actggctttc ttcaaagttg acgcgtggtg acggagtatc atataccgca ggcattagtt
    10681 cttgcccccc ttatttccag acaggggttg tatcttaagt taacattacc cgctgacgtc
    10741 gatattctcg cggagagcga taacccaacg cactatgcgg atggttttca ttgtaatgct
    10801 cgatcgccac tgcaaggtta tgcaatgccg ttcttacatt aggtttcggc atgaacgcga
    10861 tgtagtcttc cttcatcgtt ttcacgaacc gttctgccat tccattactc tgagagctac
    10921 tgattgccgt tgtgcagggc ttcagattca gctctctggc gaactgccgt gtttcatgcg
    10981 ctctgtacgc tgaaccgtta tctgtcagcc actggatgga ctgttccggt actttatcgc
    11041 caaagcgttg ttctatcgct cctaacatca catcctgcac tgtcgcttta tcgtaacctc
    11101 ccgtgcttgc ggcccagtct atggcttcac gatcgcaaca gtccaggacg aacgtaacac
    11161 gcagtttttc gccatcatcg caaccgaact caaagccatc ggaacaccaa cgctggttgc
    11221 tttccttcac cgatattcgg cctttatgtt cccgctgccg tcgtgatggt ttgtcatgta
    11281 acaacaacag actattttcg ctcattatcc ggtaaacacg ctttgcattc acgaacggta
    11341 gtccttcatt tcgggactgc cgcctgagta tcgcccagac acggcgatag agggccattc
    11401 cggcagtatg ctcagaatat ggcgcagata cgcttccgga tcgataccgt tcagacggca
    11461 ggtgcctgct tttccggcaa caagcaggaa tctcagcgct gaagcaggaa ttataggtaa
    11521 aaacgcccta ccgggccatg aatcacccgg tcattggagt agtaaccaaa gcagatttag
    11581 ccagcatgga acaaatatct ttggtgaaat gttggttacg ggaagctgga gcgcacaacg
    11641 tgttagtaac cagtgcggtt aataacaatg gagttacaga actttttgcc ttgctgcata
    11701 cagaagaagg ctgccgctaa tttcgatagg ttctcagatg agaatgatga cggtgataca
    11761 cagataagaa tcaatgagca atgagggttg ccgggcaacc ctcattgaat aaaacaggaa
    11821 ttacttcgct tcgccgtttt tcatttcgcc catagctttc agctttttgg agatatcgcg
    11881 gcgttctttg gagagctcgg catttttgat gatgtaatcg tcaacgcgat cttcatagtc
    11941 acctttcatg ctggcgatga tgccctggat tgcttcaacg ctcatccctg gcttgatgta
    12001 atcgctcagg ttgtccagca acaggacgcg cttctggttg tcacggatct ttttctcaac
    12061 gtcctgaatt tcacgttgca gtttgttctt acgacggaac agacgaacaa attccagtac
    12121 gtcctggaat gaaggcttgg tagtttccat ttttataccc ctgataatgt gagagtcgga
    12181 ttcgtttaag caaccgctat tcgttagggc caacattact catagctgcc gcagatgaca
    12241 atgcttttat cccttttcac tatcataccc tttatccttg ctgaatcgaa gcagcagcaa
    12301 gatgattctg aagttcagga attatttgcg caaggcccgg caaatcagat tcgatagcaa
    12361 ctcaagctga tgcgccgttt ccatgttcag ccacacataa ccatagattg gtgtttccac
    12421 taccttcagg tcatggtggt tattgagcaa ctgacgcagc tcttccacag catcgttcaa
    12481 tttttccgta ttggcaaaaa ccggctgcgg attaccttcg tacagcgcat gaacaaggct
    12541 cagcagtatt tgctgcatca tgtgctgggt atcacgaagt ttttgcgcgt tcaataacac
    12601 gaaatggctg gggcgcgtgg cccagtacgc attgatttgc aactccagca tacaaaccag
    12661 attgcggtta atggtctgga taccttcata tatcgatttt ggaatacggg tttctttgct
    12721 ggcgggcgca atcagcccac gcattttcac ggcatcggtc aggagttttt gtagatggct
    12781 ttccagacgt gggcgttcga gtaagttcgg tgagaatgca gattgataga cccgattata
    12841 ctcggtcaga cttttcgcca gttgaatgcg ccagtggatg aacgcccgtt gtggccagat
    12901 accggtaaac aacattgcca gtaaagagcc gaggatcaca tcgccgcttc gccataacgc
    12961 cgtatcaatt tcacctgtcg gggaaccaac aacaattgcc agcgtcaccc caatcaataa
    13021 accttgatac ggtttcttgc ccagcgccag ccaaccgcaa aggaacatgg ccgccgcgca
    13081 ccagactaac atcagcggta acgagattaa ctccagttgc agagcgataa gacctaaaat
    13141 cgaacccaac accgtaccgc caatacgctc aaaggcgcga gggacaacgt taccccagaa
    13201 cgagattggc cccataatca ccaccatggt gaccagcggc caggtgcttt ccgggatagt
    13261 aaacaggcgg ataatgagaa aagtgagcag gaatgccagc gcgacccgag taccatgcac
    13321 aatgcggtaa tggcggtata cccggatttc aaacgggctt aatgacttat cggcacgcac
    13381 gcagaaactc cgtaatgaag attaaaagcg ctcataacac atctgtcggc cagaaggcgc
    13441 gcctctgagt tatccgacgg acataccggg aacagtgtaa tttcccggca ggagcgaaaa
    13501 aattgaaagg cgcatcttac tcttttcgct ttcaaaaaaa agagtggtca tcgcgttaac
    13561 acaccgccct gagatgaatt agtgatgttt tggctgcacc gcaacataca tttcgatatc
    13621 ccagtaccca tcttccgcgc cattgttcaa ataaacctcg aagcagggct ttggtaacat
    13681 ttcataagca ctgtcctgca agaggcgatt aaagaactga taccagggtt tagcaaaatc
    13741 atcaccgact acacgagcca ccgcaaccgc atactgacca cctgaaattt ctgtcagaat
    13801 aacgccctca ctgttttcgg ggagcgtaaa gttattcggt accgtcacaa cggtgtcgca
    13861 gcgtaatttt tcggcgggtg tttcatctgg attgtcgtaa tagacagcaa cccactcctt
    13921 cggcacaata tttttgttat ctacccacat catcaactgc tcaaagcctt tctttaccgt
    13981 ctgttcccac ggaccaacga gatggaaacc tgcaacggta cgtttctctt cctgcttaat
    14041 ctcgtagttc atgacgcctc cattaacact gtttttatat acagtatagt tgcaaaatta
    14101 aaaccacaag gaatgagtgt tgattatgcg agcagactcg cactcctgcc agtctgctgc
    14161 aaaagaaagg tcaggcctta tggtggaaat aatcactcag gcgggaaaac atgccgcctt
    14221 ccccgacaga ttccagggta accagcggcc agtgcgccac ctgtttatca cggtcgtaaa
    14281 gttcaatttc ccctacccgc tgatgggcgc taattggcgc ggtgagctct ttaccatcaa
    14341 gggtatattt ggctttgata tgtggaattt cggctttcgg tagcaccatc cagaactctt
    14401 gttccgttcc caggtcgata ttttctttat cgccatacca gatgcgttcc gttccgacct
    14461 ttttcccacg gtgcaaaatt tgcaccgtag taaagttttg ttgcccccaa cgcagtaatt
    14521 ttcttgcctc ttcctcacga ccttttgcac tgtcagcccc cattaccact gcaatgagac
    14581 gacgctgccc atctacagcc gaagcaatga gattaaaccc ggcaccagaa gtatgacccg
    14641 ttttcaggcc gtcaacattc atggttttat cccacaacaa cccgttacgg ttttgctggg
    14701 tgataccgtt ccaggtgaga cttttctcac tgtacatatg ataaaactcg ggctcgccgt
    14761 ggatgatagc gcgagaaagc acagctaaat cataagccga gctatgctgg ccaggtgcat
    14821 ccagaccatg cactgtttca aaatgcgtat ccttgagatg cagcttctcg gcatagttgt
    14881 tcatcatttc aacaaactgc cgttgcccac cggcaatata gtcagccaga gcaacacaag
    14941 cgtcatttcc ggaatccaca attaaaccac ggcttaaatc acgtaccgat acgcgatcgc
    15001 cctctttcaa aaacatcagt gaagaaccga caaacaccgg attatctttc gcccacgcat
    15061 cgcgccccac ggtgacaata tcgtctggcg taatgcgatg actatcgata gcgcgatcca
    15121 cgacataacc cgtcatcagc tttgtcaggc tggcgggatt gcgctgttga tgctcattac
    15181 ccgcggtgag gatctgaccg gtggtgtaat ccatcagtac ccaggacccg gcatgaatct
    15241 gtggaggctg aggtgaaaaa ggaatgtttt ccgccgcaaa accagacgat aagttaaaaa
    15301 cgaacaaaga agcagcaata ataagacggc gtttcaacag caaaccctca ggagtttcaa
    15361 atagctgttc tttttacgga aatacttatg aactggctgg aataaagtgc aagaaaatgt
    15421 gactactctc tcatttttat ctgacatgat ctgttgccac tcgctgtcaa attgttgcgc
    15481 taaagctgat tagcacggtg atatttgata ctctggcaga cagcagaaat aacggattta
    15541 acctaatgat gaatgacggt aagcaacaat ctaccttttt gtttcacgat tacgcgaaac
    15601 acccctgaga accgccatga acaagggttt catgatttgt gattttagtt tggtacgcaa
    15661 tttggtacac aacacaattt tcacttcagc gggtagtcaa caaactcacc ataacgcgca
    15721 tcgttgatga tgtatgcgat caccacaaat atcacatctg gaccagttcc gtcttcatgc
    15781 gcaggtatct gttcacgcct ccctttctgc aaatcctaaa gatgagattt tggggtttgg
    15841 ggatatcttt tatcctcagt tctctatcca ttatgcaaac cagtaatgaa ccatcaccgt
    15901 gtgatagtga ggcatctgcc acgataagcg cagagaaatg atgcagtggt gggcgtatag
    15961 cggcctttac tttaccaatc tgtcgatgcg attaagccgg agaaaatcct tctcatatag
    16021 tttgagtcaa ttacagcgga tgtcctgatg caaagtcgct cattactatt tggcgtcgta
    16081 gcgtattgtt ctatcagaac agacgtacca gttacaacct ggtcgctacg ctgaatcgtt
    16141 gtatagccgc catcatatga gccaaaaaca gtaaagtttc cgttgacgtt ccctatgtct
    16201 ggcgttattg cgctgtattc tccaaatacg acgatgtttt tcctgaatgg aatatttgtt
    16261 acgagcatag cgagtgttcc tttaaagcct atcccgcttt tattccatcc ttccgtagtg
    16321 gttgtcctgt cagaaatttc tgaaccgctg ataacatgaa tagggtatgc cttcccccca
    16381 tggaaggtat cggagctgaa gccatcactg ttgacaactc ggtcaataat gtcattcagc
    16441 ggcttgtgcc ctatgatatg attaccacca aaaaggaaaa cctcagtctt atacggaacc
    16501 gagtacttaa tctcagcagt cgcagccaca aaagacctga aatatgcatt agatcggacg
    16561 ccaccagtta tagttttgtc accagcccca tcccatacaa aaacagcaaa ctcaccattt
    16621 ccggcgttac ggaaaacctc agcccccctt aggatttgtg taacatcatc cgtccctgcg
    16681 cctgaagatg aagtaaaagt tcccaaaaca gggatgactt ttttctgtct gctatgagcc
    16741 accattgcag aaaaggcgcg gcgcatgcgg ttcaggtcat tggcaaccct ttgatcaata
    16801 gttcctgttg attgccgaac tgcatatgga tccgccaaaa caatatcgta atattccagt
    16861 agcaaatctg aaattacgtc ggcaccatcc catgcatcaa cgatggcgat tggtttatta
    16921 gaatactggc gaatcaaatc gtaacggtct ttctgatctg catagctgaa accgcggctc
    16981 atcgcctcgt caaatgttgc ccatcccagc agtgcaggat gataccggta cttcgtcaaa
    17041 aactgcaccg tggtaagccc tgactcacca ttttcagtgc tcgcaataac ccgcatacca
    17101 agagtagcgg cattatcaag aaccttttgc acagagccgg cactatcatc tggagaggcc
    17161 gcaaagttac cataatgctt gatagtgtta atgccgagag attttatgaa tgccagttcg
    17221 ctgaggtcgt aagcagattc gacatacatg atccgcaact tatcatcgac cggcaattta
    17281 tctgtggaag atacattaac gacaccaagt gagtgcctgg acgtatctat cttaaacccg
    17341 cctataatct cctgcccttt cgattcaatt atccctttaa ccttatacgt tccagagcca
    17401 ataaccggtt ttcctgttga gaatgctgca ttgattgccg ccgtccagtc atcctcctca
    17461 acaagtgact cataatcttc aatatatgca aatgcagcgt tttgagcctg aattttgtcg
    17521 gtatcttcct tctttgcagc cccatttact aagttaacac ctccaggtgc ggataattct
    17581 tgtcgcagtt gatcaggatc atacttcagc acattaggaa aatagaactg ctgcacaccg
    17641 tacgcatcat aaacagccat agaatggcct tgcacagtta cgaacttggc aatctgtccg
    17701 ttatatacag gatacccgcc agcgttaatg atgattggct gtgcaacagg aacgtgagaa
    17761 ccgtcttcat tctctacata aacctgaatc tggttttcag gatttaccgg gtcagcgtca
    17821 atttttccga tataaatttc gccattggct acggctttaa aagaacgcgc catagtgaag
    17881 agttgcgaag gcatgcttac cacaacattt gcggtgatat ctgacatttc attgctccag
    17941 acgaatgata tgatgcaacc atgatgtgat tgcataccga aatggtacta ttgagtattt
    18001 atccagtagg ttacgatgcc attccaccca actggtgagg catcaaggat gtacagcaaa
    18061 tacgacgagg cgcagtttca cttgagactt ccgcatgaac ttcacgcgaa aattaaacag
    18121 cgtgcgaaga tgaataacag gtcgctgaac tcagagataa ttgcagcgat tgaagaatca
    18181 ttggctaaac aaagctctgc atctgtttac attgacgatg cagagcgtat ggcagaacaa
    18241 caatctgaga tggttaagaa aattgtcttt gatacgctta agaccatgta tagcaataat
    18301 aaaaaggaaa catagaaatc tagtttccgg ctaaaatggc atttccttca tgatatcctg
    18361 tgaaaaacta aggagagtta accatatgaa aaaatcactg ttaattatcc cgcttctgct
    18421 ggttggatgc gcaaaagtaa gtgactatca agcaagttgc gaacaacgct atcaaaagct
    18481 tagcgatatg gctaattgcc ttgatgccag tgtgaagaac gactcacgca tggcatcagc
    18541 accaacacct aagctgtatg tccttgctgc aaagatgctc gggcaaggtg tcgatgaagg
    18601 caagataagt gacgcacagg caagacttga gcttcagaat ctttatgttc aattacaaag
    18661 ccaagaacaa gcccaacaaa tagcacaaag ccaagcattc cagcaggctt tattgaatta
    18721 tcaggctgta aacacaatgc aagcgatcga gcaaaaagcg cgccagcctg ttataactca
    18781 accttaccca acacgcgttg acacttacac aaactgcaat tcagggtttg gaaatacagt
    18841 cacatgcaac agtagcagta acatcagata acaatcagca aaggtatcgc ctatgcagag
    18901 gaatacgata aacctcgcgt tctacatatt tggtttttgc acgttcctgg tgtttgaaag
    18961 gctattctga caacgaatca gacttagcac cctgcgtcag tgcattaatc gctttttgcg
    19021 cttgctgcat ggctttctca aacgctgtcg atccgcgtgg tgtgtttgcc attcggagca
    19081 ttgcatttct gaatggttcg ctctcatagg cgcgagtaag aagtccgtag cttaccgctg
    19141 cgccagttgt cgccgggttc attgctgtcc catacccgat aatgaacggg atggtttgct
    19201 gccctgttgg tgttgttact gccgcttttg cagcctgctg cgtggattgc aggtagtttt
    19261 ttaatccttt caggtaagca gcgtcctgcc ccttaaatgt gatgccagtc tggttttgca
    19321 ggatgttaag ctgccgaagg aactggtcag gggatccgcc agatttctcc atcgcctttc
    19381 caatgatgcc attgcgcatt tgcgccctgc caacacgacc aactgagtta tacagcgtct
    19441 taatttccga tttgttcttg ctgaatagca tgttgttgac aacttccggc gtcaggtctc
    19501 ctttcatgag aacattcttc agcctggtat tctttagttt cgccgcttcg tcagcgtaga
    19561 cggcattggc ctgctgatat ttacggagag tatcgttgcc aagattctga ccaatggcgc
    19621 cattgatatc atctgtcatc gccttgtaaa cgcgctgaat ggcggcatcg gaacggtttg
    19681 gtaacactgg tcgctcaccc t
//
LOCUS       DAZKHC020000076        19682 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZKHC020000076 DAZKHC020000000
VERSION     DAZKHC020000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.076"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..158
                     /locus_tag="ACHOSM_RS16645"
                     /old_locus_tag="pgaptmp_003329"
     CDS             <1..158
                     /locus_tag="ACHOSM_RS16645"
                     /old_locus_tag="pgaptmp_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559781.1"
                     /translation="FLSDNTLEKDENSPDNCFEVVKYNTYEAYNSEKQYFTREDYTYD
                     YNLLNAI"
     gene            complement(542..918)
                     /gene="rnpB"
                     /locus_tag="ACHOSM_RS16650"
                     /old_locus_tag="pgaptmp_003330"
     ncRNA           complement(542..918)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHOSM_RS16650"
                     /old_locus_tag="pgaptmp_003330"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(951..2096)
                     /gene="garK"
                     /locus_tag="ACHOSM_RS16655"
                     /old_locus_tag="pgaptmp_003331"
     CDS             complement(951..2096)
                     /gene="garK"
                     /locus_tag="ACHOSM_RS16655"
                     /old_locus_tag="pgaptmp_003331"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HGU4559782.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASESM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2193..3083)
                     /gene="garR"
                     /locus_tag="ACHOSM_RS16660"
                     /old_locus_tag="pgaptmp_003332"
     CDS             complement(2193..3083)
                     /gene="garR"
                     /locus_tag="ACHOSM_RS16660"
                     /old_locus_tag="pgaptmp_003332"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU4559783.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3113..3883)
                     /gene="garL"
                     /locus_tag="ACHOSM_RS16665"
                     /old_locus_tag="pgaptmp_003333"
     CDS             complement(3113..3883)
                     /gene="garL"
                     /locus_tag="ACHOSM_RS16665"
                     /old_locus_tag="pgaptmp_003333"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HGU4559784.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3899..5233)
                     /gene="garP"
                     /locus_tag="ACHOSM_RS16670"
                     /old_locus_tag="pgaptmp_003334"
     CDS             complement(3899..5233)
                     /gene="garP"
                     /locus_tag="ACHOSM_RS16670"
                     /old_locus_tag="pgaptmp_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HGU4559785.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5608..7179
                     /gene="garD"
                     /locus_tag="ACHOSM_RS16675"
                     /old_locus_tag="pgaptmp_003335"
     CDS             5608..7179
                     /gene="garD"
                     /locus_tag="ACHOSM_RS16675"
                     /old_locus_tag="pgaptmp_003335"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HGU4559786.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQDIPLENASIVSLQDEKHVGFQSMVEDILQIAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7328..7663
                     /gene="prlF"
                     /locus_tag="ACHOSM_RS16680"
                     /old_locus_tag="pgaptmp_003336"
     CDS             7328..7663
                     /gene="prlF"
                     /locus_tag="ACHOSM_RS16680"
                     /old_locus_tag="pgaptmp_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HGU4559787.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7663..8127
                     /gene="yhaV"
                     /locus_tag="ACHOSM_RS16685"
                     /old_locus_tag="pgaptmp_003337"
     CDS             7663..8127
                     /gene="yhaV"
                     /locus_tag="ACHOSM_RS16685"
                     /old_locus_tag="pgaptmp_003337"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HGU4559788.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEENH"
     gene            complement(8182..8991)
                     /gene="agaR"
                     /locus_tag="ACHOSM_RS16690"
                     /old_locus_tag="pgaptmp_003338"
     CDS             complement(8182..8991)
                     /gene="agaR"
                     /locus_tag="ACHOSM_RS16690"
                     /old_locus_tag="pgaptmp_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HGU4559789.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9240..10520
                     /gene="kbaZ"
                     /locus_tag="ACHOSM_RS16695"
                     /old_locus_tag="pgaptmp_003339"
     CDS             9240..10520
                     /gene="kbaZ"
                     /locus_tag="ACHOSM_RS16695"
                     /old_locus_tag="pgaptmp_003339"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HGU4559790.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYYTACEGQ"
     gene            10543..11016
                     /gene="agaV"
                     /locus_tag="ACHOSM_RS16700"
                     /old_locus_tag="pgaptmp_003340"
     CDS             10543..11016
                     /gene="agaV"
                     /locus_tag="ACHOSM_RS16700"
                     /old_locus_tag="pgaptmp_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HGU4559791.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            11027..11806
                     /gene="agaW"
                     /locus_tag="ACHOSM_RS16705"
                     /old_locus_tag="pgaptmp_003341"
     CDS             11027..11806
                     /gene="agaW"
                     /locus_tag="ACHOSM_RS16705"
                     /old_locus_tag="pgaptmp_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HGU4559792.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11796..12674
                     /gene="agaE"
                     /locus_tag="ACHOSM_RS16710"
                     /old_locus_tag="pgaptmp_003342"
     CDS             11796..12674
                     /gene="agaE"
                     /locus_tag="ACHOSM_RS16710"
                     /old_locus_tag="pgaptmp_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HGU4559793.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12692..13126
                     /gene="agaF"
                     /locus_tag="ACHOSM_RS16715"
                     /old_locus_tag="pgaptmp_003343"
     CDS             12692..13126
                     /gene="agaF"
                     /locus_tag="ACHOSM_RS16715"
                     /old_locus_tag="pgaptmp_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HGU4559794.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSDEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            13123..14256
                     /gene="nagA"
                     /locus_tag="ACHOSM_RS16720"
                     /old_locus_tag="pgaptmp_003344"
     CDS             13123..14256
                     /gene="nagA"
                     /locus_tag="ACHOSM_RS16720"
                     /old_locus_tag="pgaptmp_003344"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU4559795.1"
                     /translation="MTPVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTARLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14607..15761
                     /gene="agaS"
                     /locus_tag="ACHOSM_RS16725"
                     /old_locus_tag="pgaptmp_003345"
     CDS             14607..15761
                     /gene="agaS"
                     /locus_tag="ACHOSM_RS16725"
                     /old_locus_tag="pgaptmp_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HGU4559796.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15774..16634
                     /gene="kbaY"
                     /locus_tag="ACHOSM_RS16730"
                     /old_locus_tag="pgaptmp_003346"
     CDS             15774..16634
                     /gene="kbaY"
                     /locus_tag="ACHOSM_RS16730"
                     /old_locus_tag="pgaptmp_003346"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HGU4559797.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESVFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16799..17275
                     /gene="agaB"
                     /locus_tag="ACHOSM_RS16735"
                     /old_locus_tag="pgaptmp_003347"
     CDS             16799..17275
                     /gene="agaB"
                     /locus_tag="ACHOSM_RS16735"
                     /old_locus_tag="pgaptmp_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HGU4559798.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17314..18117
                     /gene="agaC"
                     /locus_tag="ACHOSM_RS16740"
                     /old_locus_tag="pgaptmp_003348"
     CDS             17314..18117
                     /gene="agaC"
                     /locus_tag="ACHOSM_RS16740"
                     /old_locus_tag="pgaptmp_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HGU4559799.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSQLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            18107..18898
                     /gene="agaD"
                     /locus_tag="ACHOSM_RS16745"
                     /old_locus_tag="pgaptmp_003349"
     CDS             18107..18898
                     /gene="agaD"
                     /locus_tag="ACHOSM_RS16745"
                     /old_locus_tag="pgaptmp_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HGU4559800.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSAALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18899..19654
                     /gene="agaI"
                     /locus_tag="ACHOSM_RS16750"
                     /old_locus_tag="pgaptmp_003350"
     CDS             18899..19654
                     /gene="agaI"
                     /locus_tag="ACHOSM_RS16750"
                     /old_locus_tag="pgaptmp_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HGU4559801.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGHPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATECFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         4795 a         5122 c         4995 g         4770 t
ORIGIN      
        1 aattcctttc agataataca ctcgagaaag acgaaaattc tcctgacaat tgctttgagg
       61 ttgtaaaata taacacatat gaagcatata actcagagaa gcaatatttt accagagagg
      121 actatacgta tgattacaac cttttaaatg caatatagac ttaataactt cttacaaagt
      181 ctattattaa taccgtagat attcatctta ccctgtgcac caaaagcaaa aactcactga
      241 cgcgggtttg aaactgatgg caaccgcaaa aatgcctgat gcgctacgct tatcaggcct
      301 acgccatctc tgcaatatat tgaatttgcg tgcttttgta ggcaggataa ggcgttcacg
      361 ccgcatccgg catgaataaa gcgcactcaa caatctgaaa cccgccggaa cggtttatta
      421 cgtacatcag gtgaaactga ccgataagcc gctttctttt gggtatagtg tcgtggacag
      481 tcattcatct ttctgcccct ccaaaagcaa aaacccgccg aagcgggttt ttacgtaaat
      541 caggtgaaac tgaccgataa gccgggttct gtcgtggaca gtcattcatc taggccagca
      601 atcgctcact ggctcaagca gcctacccgg gttcagtacg ggccgtacct tatgaacccc
      661 tatttggcct tgctccgggt ggagtttacc gtgccacgga ctgttaccag ccgcgcggtg
      721 cgctcttacc gcaccctttc acccttacct gatcccgctt gcgcgggcca tcggcggttt
      781 gctctctgtt gcactggtcg tgggtttccc ccccaggcgt tacctggcac cctgccctat
      841 ggagcccgga ctttcctccc ctccgcccgt ctcccccgaa gaggacgacg acgaagcggc
      901 gactgtctgg tcagcttcgg cgcgcagtat agagggtttg cgcgcccttg tcaccccgcg
      961 ttgcgcattc caatcgccag tgtcgcggcg atattacgtg aagcacggca gatattgtca
     1021 taagccccgc ggaatgcttc gtccaacgta cctatgctgg tcaatacgct gaagaccgca
     1081 tcaatgccat gctgatgtac aacgccaaca tcatcggtca ggctacccgc aatgccaatc
     1141 accggtttat ggtacttctt cgccacgttt gcgacgccaa tcggtacctt gccgtgaata
     1201 ctctggctgt caatacgccc ttcaccggtg atcaccagcg tacaatcgtg aatatgttcc
     1261 tccagattca gcgccgtagt gacgatttca ataccacttt tcagttccgc accaagaaac
     1321 gccatcagcg ccgcgcccat accgccagca gctcctgcgc cggggacatc tttcacatca
     1381 acatgcagcg cttttttaat gacatcggca tagtgagaga ggttattgtc cagctcaaca
     1441 atcatcgatt cactggctcc cttttgtggg ccaaagatgc gcgatgcgcc gttatcgccc
     1501 accagcggat tggtgacatc acaagcgacg cgaatgacgc aatcttttaa gcgcggatcg
     1561 aggccggaaa tatcaatatc attcagagta ttaagactac cgccgccaaa accaatttca
     1621 ttgccgttgg cgtcgcataa tttcgccccc agcgcctgta ccatgcctgc gccgccatca
     1681 tttgtagcgc tgccgccaat gccgataata atgtttgtcg cgccgctctc cagcgcctgc
     1741 aggattaact cgcctgtgcc gcgtgaagtg gtcacgagtg gatcgcgttt ttccgcaggt
     1801 accagctcca gcccactggc cgccgccatt tcaataaacg cggttttgcc atcgccggag
     1861 atcccccaac tggcattcac tttctcgccc agcggccctg taacccaggc gtgacgttcg
     1921 gaaccctggg tggctgcaat catcgcttcc accgttcctt cgccaccgtc ggcaaccgga
     1981 acagaaacgt actgtgcatc aggaaaaatt tcccgaaatc ctttttctat cgcctgcgca
     2041 acctcgctgg cagataaact ttctttataa gagtctgggg cgattacgat tttcatacct
     2101 atgcctgtta ccacatgacg ccggagggcg tttctcttat tcggcctgga ttccaggccc
     2161 ggattgcaat acgccatccg ggcacgacgt cattaacgag taacttcgac tttcgccagt
     2221 ttttcgtagt agcacgccag agcgctatga tccgccgttc ctaaaccatc tgcacgcagt
     2281 gcctgcatca tctccataac cgcagctgtg agcggcagtt gcgcgccgac gccgtgagaa
     2341 gtatccagcg cattcgccag atccttaata tgcagatcaa tgcggaagcc cggcttgaag
     2401 ttgcggtcca tcaccatcgg cgctttggca tccagcacgg tactgcccgc cagcccaccg
     2461 cgaattgcct gataaaccag gtccgggtta acgcccgctt tagttgccag cgttaacgct
     2521 tctgacatcg cagcaatatt cagcgccaca atgacctgat ttgccagttt ggtgacgtta
     2581 cctgcaccga tttccccggt atgcaccacg gaacccgcca tcgctttcat caaatcatag
     2641 tatttgtcga aaatagcctt gtcgccgccc accatcactg acagcgtacc gtcgatggct
     2701 ttcggttcac cgccgctcac cggagcatcc agcatatcaa tgcctttcgc tttcagcgct
     2761 tcgctgattt cacggcttgc cagcggtgcg atagaactca tatcgatcaa taccgtacct
     2821 ggcttcgcgc cttcaataat gccattctca cccagcgcca cctctttcac atgaggggag
     2881 tttggcagca tggttatgat gacgtcgcac tgttcagcga tcgctttagc cgtagacgct
     2941 gtttctgcac ctgcagcaat cacgtcagca atagcttctg ggttacggtc agcaaccacc
     3001 agcgagtaac ctgctttcag aaggttttta ctcattggtt tacccataat ccccaggcca
     3061 ataaaaccaa ctttcatagt catatcaatc atctctcttg ttgcggtggt ggttattttt
     3121 taaaggtatc agccagtttc tgagtggcag agcggaagac gccaagatcg ctgccgacag
     3181 ccacaaacgt cgcgccccat tccagataac gacgcgcatc ggcttcgacc ggcgcgagaa
     3241 taccgctggg tttgccgtgc gcactggcac ggttaaaaat gtgctgaatt gctttttgta
     3301 catccgggtg cgaggcattg ccgagatggc ctaatgccgc agccagatcg ctggggccga
     3361 cgaagatacc gtctacacct tcggtagcgg caatggcatc gacgttatct acgccctgct
     3421 gactttctat ctggaccaga atggtgatgt tcttgttcga ctgagcgaaa taatccgcca
     3481 cggtgccaaa catattggcg cggtgagaaa cggagacgcc gcgaatgcct tccggtgggt
     3541 aacgggttga tgccaccgcc tgctctgctt cctcttttgt ttctacaaaa ggaatcagga
     3601 agttatagaa accgatatcc agaagacgct taataattac cggctcgttg gtcggcactc
     3661 gcactactgg cgcgctggcg ctgcctttca gggccattaa ctgcggaata aacgtggaga
     3721 tatcgtttgg cgcatgttcg ccatccagca ccagccagtc aaacccagcc aaaccaagaa
     3781 cttcagtgct aatcgggtta gagagtgctg accagcaacc aatttgtacc tgtttcgcag
     3841 ccagtgcggc tttgaattta ttcgggaaaa catcgttatt catcgcttat acctttgctt
     3901 atttctgcaa ttccatacgt ttaatgtcgc caactacgaa gaggtagcag accatcgcca
     3961 tcagcgctga acatcccacg aaaaccagtg ctgcattgaa ggagtgcagt tcacttacca
     4021 ggtagccaat caccagtgga gtgacaatgg aggcaacatt gccaaagacg ttaaagacgc
     4081 cgccacagag gccaacaatc tctttcggcg cggtgtcaga aatcaccggc cagcccagag
     4141 caccaaatcc tttgccaaaa aaagccagcg ccatcagcat gaccaccagc gtggtgttgt
     4201 tggtgtagtt acataagatg atggtggaag ccagcaacat tcccagcaca atcggtagct
     4261 tacgtgccag ggtcagggat aaaccgcgtt tgatcagata atccgagaag acacctccca
     4321 gcacgccgcc cgcaaaacca cacagtgccg gaatcgaggc gaccagaccc actttcagaa
     4381 tcgacatgcc tttttcctgc accagataaa tcgggaacca ggtgaggaag aaccaggtga
     4441 tggtgttgat aaaatattgt ccgaaaaata cgcccaacat catgcggtta gagagcaatt
     4501 gcttgatgta atgcagtttg ggaccgcttg ctgccgcgct gcccggcttt ttgtggtcca
     4561 tatcgaccac cgcgccattt tcagagataa acttcagctc ttcctcagac atacgtgggt
     4621 gatctgtcgg gttatgaatc aacttgatcc acagcgccgt cagcacaaaa ccaatcaccc
     4681 ccataacggt aaagacgtgc tcccatcccc aggcgaaagt cagccagcca agcagaggcg
     4741 aaaagagcgc cagcgagaaa tattgtgccg agttaaagat ggcggaggca gtaccacgtt
     4801 ctttcgtcgg gaaccaggcg gcgacaatac gggcgttcgc cgggaatgat ggcgcttccg
     4861 agaatcccag cataaagcgc ataaagaaca tggagatccc tgcccaggcc agcgggaaca
     4921 tatcaacaaa gccttgcagg aaggtgaaca gcgaccagaa aaagaggctg taggtgtaaa
     4981 cttttttcga gccaaactta tcaagcaacc agccgccggg gatttgcatc agcaagtagg
     5041 cccagccaaa agcggagaag atgtaaccca tcgaaaccgc acttaactgc aactcttttg
     5101 ccacttcggt accagcaata gacagcgttg cacgatcggc gtagttaacg gcggtaacaa
     5161 taaaaataat cagtaatatt aaatagcggg tatgcacgcc tttctttttt acgtcaacgg
     5221 tgtccagaat cattttattt acctcgggta cttatgctga tttttattat tatggtgaag
     5281 gtgttattta tgagttttat ttatgccgtt acgacaatga actcgggaat tagtataagc
     5341 agcgcgagaa taataatcat tgtgcaaatg ctaatttaat taatactatt taaatattat
     5401 tttgagcata tgcacataag gttgcgcgct aaagcacaga tttgcgcttt accttgccgg
     5461 gcggcactgc aatccctgaa atgattgaca ttgatcacat ttctgcgttt aaactcctga
     5521 cattcttatt tcacccaatg aagtcattta tttttaaatg agaccaggtc ctcattttaa
     5581 taacccctgg ctggagaata ttgcacaatg gccaacatcg aaatcagaca agaaacgcca
     5641 actgcgtttt atataaaagt tcacgacaca gataatgtgg caattattgt taatgataat
     5701 ggcctgaaag caggaacgcg ttttccggat gggctggaat taattgaaca tattccccaa
     5761 gggcataaag tcgcattgct ggacattccg gctaatggtg aaattattcg ttatggcgaa
     5821 gtgattggtt acgccgtgcg cgcaatccca cgcggaagct ggatcgatga atcaatggtt
     5881 gtactaccgg aagcgccgcc gttacacacg ctgccactgg caaccaaagt gccggaaccc
     5941 ttaccgccgc ttgaaggtta cacctttgaa ggctatcgca atgccgatgg cagcgtgggc
     6001 accaaaaacc tgctcggcat caccaccagc gtccactgtg tggcaggcgt ggtggactac
     6061 gtagtaaaaa tcattgaacg cgatctgctg ccgaaatacc cgaacgtcga tggcgtggtg
     6121 gggctgaatc atctgtacgg ttgtggcgtg gcgattaacg ctccggcggc ggtggtgccc
     6181 attcggacca ttcacaacat ttcgcttaac ccaaactttg gcggcgaggt gatggtgatt
     6241 ggcctgggtt gcgagaaatt acagccagaa cgcctgctgg tcggcacgga tgatgtacaa
     6301 gatattccgc tggagaacgc cagtattgtc agcttgcagg atgaaaagca tgtcggtttt
     6361 cagtccatgg tcgaggatat tttgcagatc gccgaacgcc atctacaaaa actgaatcaa
     6421 cggcagcgag aaacctgccc ggcttcagaa ctggtcgttg gtatgcagtg cggtggcagc
     6481 gatgcgtttt ctggtgtaac ggcaaacccg gcggttggct atgcgtctga tctactggtg
     6541 cgctgcggcg caacggtgat gttttcagaa gtaacggaag tgcgtgacgc gatccatctg
     6601 ctgacaccac gcgcagtgaa cgaagaggtc ggcaaacggc tgctggagga gatggagtgg
     6661 tacgataact atctcaatat gggaaaaacc gaccgcagcg ccaacccttc gccgggcaac
     6721 aagaaaggcg gtctggcaaa tgtggtggag aaggcactcg gctccattgc taaatcgggt
     6781 aaaagtgcaa ttgttgaagt gctgtcgccc ggtcaacgcc cgactaaacg cggattaatt
     6841 tacgccgcga cgccagccag cgattttgtc tgtggcacgc aacaggtggc ttcgggtatc
     6901 accgtacaag tgtttacgac cggccgtggt acgccgtacg gcctgatggc ggtacccgtc
     6961 attaaaatgg caacccgcac cgagctggcg aaccgctggt ttgatttaat ggatattaac
     7021 gcgggcacta tcgctaccgg cgaagagagc attgaagagg tgggctggaa gttgttccac
     7081 tttattctcg acgtcgccag cgggaagaag aaaaccttct cggatcaatg ggggctgcat
     7141 aaccagctgg cggtgtttaa cccggcaccg gtgacctgat ttcttttgga acgtccctcg
     7201 caaaacatgg ccttagtgcc atgtttttat tgtttaaagc ccccacgtcc attaataatg
     7261 catttgcatt accttagttc aagcttataa tttgagcaga aaacaggctg taaaaggaca
     7321 gtgaatcatg cccgctaatg ctcgctctca cgctgtactg accactgaat caaaggtcac
     7381 gatacgcgga caaacaacta tccccgcgcc agtgcgtgag gccttaaaac tgaagccagg
     7441 ccaggacagc attcattacg aaattctgcc tggtgggcaa gtatttatgt gccgactggg
     7501 agatgaacag gaggatcata ctatgaatgc atttttgcgt tttctggata cagatatcca
     7561 gaacaacccg caaaaaactc gtccattcaa cattcaacaa ggaaagaaac ttgtcgctgg
     7621 catggacgtc aacattgatg atgagattgg cgacgacgaa taatggattt tccacaaagg
     7681 gttaatggtt gggcgctata tgctcatccc tgttttcagg aaacctacga cgctttagtt
     7741 gccgaagtcg agacattaaa gggaaaagat cctgaaaatt atcagagaaa agccgccaca
     7801 aagttattgg cggtagtcca taaagtgatt gaggagcata tcacggtcaa tccatcatca
     7861 ccggcattcc gtcatggcaa gtcgttaggc tctgggaaaa ataaagactg gtcacgggta
     7921 aaatttggtg ctggtcgtta tcgtctcttc tttcgttata gtgaaaaaga gaaagtcatc
     7981 attctgggat ggatgaacga tgaaaacact ctgcgcacct acggtaaaaa aacagatgcc
     8041 tataccgtat tcagcaaaat gttaaaaaga ggacatcctc ctgccgactg ggaaaccctc
     8101 acccgagaaa cagaagaaaa ccattgatgg tgttcacaat gcccgccgaa cgcattcgtg
     8161 ttgaccagcg ggcacccttt ctcactcccc caccagaatc acttcaaccc cagcctttcg
     8221 cagtccttcc aggctatccg caggaatgcc ttcatcaaca atgatcatgt cgatacgttg
     8281 agtatcaatg atcttatgta aactggaacg attgaattta ctggaatcgg tgaccacgat
     8341 gatccgttcc gcaacttcgc acatccgacg gtttaaacgg gcttcatctt cattatgtgt
     8401 gctgacgccg cgctccagat cgatcgcatc tacaccaaga aacagcatat cgaagtggta
     8461 attttgcagc gattgctcag cctgatcgcc gtaaaaagat tgcgactgac ggcgcaaatg
     8521 cccgccggtc atcagcagct caacgccttc cgcttccagc aacgcattag ccacgttcat
     8581 accgttggtc atcgcaatta cgtcagtgtg cttacgcatc agacgagcaa tctcaaaagt
     8641 ggtagtcccg gaatcgagga tcacccgatg accaggctga atcaactcaa cggcagcttt
     8701 cgcaacgctg cgtttcatcg cggtgttcag cgcgctttta tcttccactg atggctcgac
     8761 tgacggcgtc gtgctatcgc agatcaacgc gccaccatag gcacgcacag cgatcccctg
     8821 cttttccaga aacgccagat cattgcggat cgtcacagta gatacgccat acaatgccga
     8881 cagatcgtta acctgcacac tcccttgctg tcgcagacgc tgaatgatct gttctcgtcg
     8941 ctcgctggtg ccagtcactc gcttctcacc tgaagcgtcg gtattactca tagtaagtcc
     9001 tttcgtaaaa ctttcgtttc atttcgtttt gcctattaac gcctttctat taagcaaatg
     9061 caagcccacc ttgcccattg acgcaagcta ctctcgtttc agtgactttc attgtgtttc
     9121 ttttgtgaat cagatcagaa aactattatc tttcgtttta tttttatctc accatgacgc
     9181 agtatcaact gaaacaaaac gaaagattaa tatcgcagta atctgaactg gagaggaaag
     9241 tgaaacatct gacagaaatg gtgagacagc acaaagcggg caaaacaaat ggaatttatg
     9301 ccgtttgttc cgcacatccg ctggtgctgg aggctgcaat ccgctacgcc agtgcaaacc
     9361 aaacgccgtt actgattgaa gcaacctcca atcaggtaga ccagttcggc ggttataccg
     9421 gaatgacgcc cgccgatttt cgcggctttg tttgtcagct cgccgactcg ttgaatttcc
     9481 cgcaggatgc gttgattctg ggtggtgacc atctggggcc aaaccgctgg caaaacctgc
     9541 cagccgctca ggcaatggcc aatgccgatg atttgattaa aagctacgtt gcggcaggat
     9601 tcaaaaaaat ccaccttgat tgcagcatgt cctgtcagga cgatccaatt cccttaactg
     9661 atgacatcgt ggctgaacgc gccgcccgtc tggcgaaagt ggcggaagaa acctgtcgcg
     9721 aacactttgg cgaagccgat ttggagtatg tcattggtac cgaagtgccg gtacctggcg
     9781 gcgcgcatga aaccttaagc gagctggcgg tcaccacgcc ggatgccgcc cgcgccacgc
     9841 tggaagccca tcgtcacgcc tttgaaaagc aaggtttgaa tgccatctgg ccacgcatca
     9901 ttgccctggt ggttcaaccc ggcgtcgaat tcgatcacac caacgttatt gattatcagc
     9961 ccgccaaagc gagcgcctta agccagatgg tcgaaaacta cgaaacgctg attttcgaag
    10021 cgcactctac cgattatcaa acgccgcaat cgctgcgcca gctggtgatt gaccactttg
    10081 ccattctgaa agttggcccg gcgctgacct tcgctctgcg tgaagccctt ttttctctgg
    10141 cggcgattga agaagaactg gtaccagcga aagcctgttc cggtctgcgt caggtgctgg
    10201 aaaacgtgat gctcgaccgc ccggaatact ggcaaagcca ctaccacggt gacggcaacg
    10261 cgcgtcgtct ggcgcgtggt tatagctact cggatcgcgt gcgctattac tggccggaca
    10321 gccagattga tgacgctttc gcccatctgg ttcgtaatct ggcggattca ccgattccgc
    10381 tgccgctcat cagccagtat ctgccgctgc aatacgtgaa agttcgctcc ggcgagctgc
    10441 aaccgacgcc acgggaactc attatcaacc atattcagga catcctggcg cagtactaca
    10501 cagcctgtga aggccaataa gcaaaacaaa gaggaacacg ctatgccaaa tattgtttta
    10561 agccggattg atgaacgctt gattcacggt caggtcggcg ttcagtgggt cggatttgcg
    10621 ggggcaaatc tggtgctggt agccaacgat gaggttgccg aagatccggt acaacaaaac
    10681 ctgatggaaa tggtactggc agaagggatc gccgtacgtt tctggacgct gcaaaaagtt
    10741 atcgacaaca ttcatcgcgc cgccgatcga cagaaaatcc tgctggtttg taaaacaccc
    10801 gccgatttcc tgacgctggt gaaaggtggc gttccggtga atcgcattaa cgttggcaat
    10861 atgcactacg ccaatggcaa acaacaaatc gccaaaacgg tttctgtgga tgcgggcgat
    10921 atcgcagcat ttaacgacct gaaagccgct ggggtggaat gcttcgttca gggcgtcccg
    10981 acagagcctg ctgtggacct ctttaaatta ctttgaggga ttcatcatgg aaatcagcct
    11041 gttgcaggca tttgcgttgg gcattatcgc ctttatcgct ggcctggata tgtttaacgg
    11101 cttaacccat atgcaccgcc cggtggtcct cggcccgttg gtcgggctgg tacttggcga
    11161 tctgcatacc ggaattttaa ccggcggtac gctggaactg gtgtggatgg ggctggcccc
    11221 gctagcgggc gcacagccgc ctaacgtgat tatcggtact atcgtcggca cggcgtttgc
    11281 cattactact ggcgtgaaac ccgatgtcgc ggtcggtgtc gccgtacctt tcgctgtcgc
    11341 agtacagatg gggattacct tcctgttctc ggtgatgtct ggcgtgatgt ctcgctgcga
    11401 ccggatggcg gagaacgccg atactcgcgg cattgaacgc gtgaactatc tggcactgct
    11461 ggcactcggc actttctatt ttctctgcgc tttcctgcct atctacttcg gcgcggaaca
    11521 tgcaaaaacc atcattgatg tcctgccgca acgattaatc gacggcctcg gcgtcgcggg
    11581 cggcatcatg ccagcaatcg gttttgccgt gctgctgaaa atcatgatga aaaacgtcta
    11641 catcccctac ttcatcctgg gctttgttgc cgcagcctgg ctcaagttac cggtgctggc
    11701 tattgctgcc gctgcgctgg cgatggcgct gatcgacctg ctgcgtaaat ctcctgaacc
    11761 gactcaacct gcggcacaga aagaggaatt tgaagatggc atctaatcaa accaccctgc
    11821 cgaacgtcag tgaaaacgaa gaaacattgc tgactggcgt caatgaaaac gtatatgaag
    11881 atcagagcat tggcgcggag ctgacgaaaa aagatatcaa ccgcgtcgcc tggcgttcca
    11941 tgctgttaca agcgtctttt aactacgaac gtatgcaggc ttctggctgg ttgtacggtc
    12001 tgctgcctgc tctgaaaaag atccacacta ataaacgcga cctggcgcgc gccatgaagg
    12061 ggcatatggg tttcttcaat acccatccgt ttctggtgac gtttgttatc ggcattatcc
    12121 ttgcgatgga gcgttctaag caggacgtta acagtattca gagcaccaaa attgccgtcg
    12181 gtgcgccgct cggcgggatt ggtgatgcga tgttctggct gacgctactg ccgatttgtg
    12241 gcgggatagg cgcaagcctt gcgctacaag gctctattct tggcgcagtc gtatttattg
    12301 tgctgttcaa cgtggtacac ctggggctgc gttttggtct ggcgcattat gcttaccgca
    12361 tgggcgtggc ggcgattcca ctcattaaag cgaataccaa aaaagtcggc catgctgcgt
    12421 ctatcgttgg gatgacggta atcggcgcgc tggtggcaac ctatgttcgt ttaagcacca
    12481 cgctggaaat cacagcgggc gacgcagtgg ttaagttaca gactgatgtt attgacaaac
    12541 tgatgcctgc cttcttaccg ctggtctaca ccttgaccat gttctggctg gtacgccgcg
    12601 gctggagtcc gctgcgcctg attgcagtta ccgtggttct cggcatcgtc ggtaaattct
    12661 gccatttcct ttaaatacaa tgaggtatta gatgttaagt attattttga cagggcatgg
    12721 cgggtttgcc agtggcatgg aaaaagcaat gaagcaaatc ctcggcgagc aatcgcagtt
    12781 tattgccatc gattttcccg aaacctcaag caccgcgctg cttacatcgc agcttgaaga
    12841 ggccatcgcc caactggatt gtgaagacgg cattgttttt ctgactgatt tgctgggcgg
    12901 cacgccgttt cgcgtagctt caacgctggc gatgcaaaaa ccgggctgtg aagtgatcac
    12961 cggcaccaat ttacaactgc tactggagat ggtgctggag cgcgaagggt taagcgatga
    13021 agagttccgt gtgcaggcgc tggagtgtgg acatcgtggg ctgaccagtc tggtagacga
    13081 gttaggccgc tgccatgaag aatgtccggt cgaggaagga atatgacacc cgttctgcgc
    13141 gccagaaggc tgctgactga agagggatgg ctcgatgacc atcagttgcg tattgctgac
    13201 ggtgtcatcg cagcaatcga accgattcca gtgggcgtga ctgaacgcga tgcggaactg
    13261 ctctgccctg cttacatcga cacccatgta cacgggggtg cgggcgttga tgtaatggat
    13321 gacgcgccgg atgtactcga caagctggca atgcacaagg cacgcgaagg tgtcggcagt
    13381 tggttaccga ccaccgtaac cgcgcggctt aataccattc atgcggcgct gaaacgtatt
    13441 gctcaacgtt gccaacgcgg cggacctggt gcgcaagtgc tggggagtta tctcgaagga
    13501 ccgtacttca cgccgcagaa taaaggcgcg catccgccgg agttgtttcg cgagcttgaa
    13561 attgccgagc tggatcagtt gattgccgtt tctcagcaca ccttacgcgt ggtagcgctg
    13621 gcaccggaaa aagaaggggc attgcaggcc atccgccatc ttaaacagca aaacgtaagg
    13681 gtgatgctgg ggcatagcgc ggcgacctgg caacaaaccc gcgccgcatt tgatgctggt
    13741 gccgacggcc tggtgcattg ctataacggg atgacaggtt tacatcaccg cgaaccggga
    13801 atggttggcg cgggattaac ggacaagcgc gcctggctgg aactgatagc cgatggtcat
    13861 catgtgcatc cggcggcaat gtcgctgtgt tgttgctgtg cgaaagagag aatcgtactg
    13921 atcaccgacg cgatgcaggc agctgggatg ccggatggtc gctatacgtt atgtggtgaa
    13981 gaagtgcaga tgcacggtgg cgttgtccgt accgcgtctg gtgggctggc gggcagtacg
    14041 ctgtctgttg atgcggcagt gcgcaatatg gtcgagttga cgggcgtaac gcctgcggaa
    14101 gccatccata tggcgtcgct gcatccggcg cgaatgctgg gtgttgatgg tgttctggga
    14161 tcgcttaaac cgggcaaacg cgccagcgtc gttgcgctgg atagcgggct acatgtgcaa
    14221 caaatctgga ttcagggtca attagcttcg ttttgatggt ttgctccatt attgggcctt
    14281 cacttccccc gtaaggcctt tctttttctt tctttttgat ctgtgcagcg gtgtcggatg
    14341 cgacgctaac gcgtcttatg cgacctacgg tacgccgcat ccggcaatcg gtgaacatag
    14401 cgacgtcatt acgcaattct ttaatcttcc cttgtctttt ctttcaacga tcacaaattt
    14461 cgttttattt cctttttctc cattgaactt tcagtttctt ttctatagat tttaatcaac
    14521 gaaagacatc accaagtgaa atgaaacgaa aggcaagtga aagcgacaac gcccgacgtc
    14581 aagttcatca gactaaggat tgagttatgc cagaaaatta cacccctgct gccgccgcaa
    14641 ccggtacatg gactgaagaa gagatccgcc atcagcctcg cgcatggatc cgttcactca
    14701 ccaacatcga cgcgctacgt tccgcgctca ataacttcct tgaaccgtta ctgcgcaaag
    14761 agaatctgcg gatcatcctg accggagccg gaacgtcggc atttatcggt gacatcatcg
    14821 cgccgtggct cgccagccat accggtaaaa acttcagcgc cgtaccgacc accgatctgg
    14881 tcaccaatcc gatggactac ctgaacccag cccatccgct gctgttgatc tccttcggtc
    14941 gatccggcaa cagcccggaa agcgtcgctg ccgtggaact ggcaaatcaa tttgtaccag
    15001 aatgctatca cctgccgatc acctgcaacg aagcgggcgc tctttaccaa aacgcgatca
    15061 acagcgataa cgcgtttgcc ctgctgatgc ccgcagaaac gcacgatcgc ggctttgcga
    15121 tgaccagcag cattaccacc atgatggcca gctgcctcgc ggttttcgca cctgagacga
    15181 tcaacagcca aaccttccgc gacgtggcgg atcgttgcca ggcgatcctg acctcactgg
    15241 gcgatttcag cgaaggtgtg tttggttacg caccgtggaa acggatcgtt tatctcggca
    15301 gcggtggctt acagggcgca gcacgcgagt cggcgctgaa agtgctggaa ctgacggcgg
    15361 gtaaactggc ggccttttat gattctccaa ccggattccg tcatggacca aaatcgctgg
    15421 tcgataacga aacactggtg gtggtatttg tctccagcca cccttacacc cgtcagtatg
    15481 atcttgatct gctggctgaa ctccgccgtg acaaccaggc aatgcgcgta atcgccatcg
    15541 ccgcggaaag caccgacatc gtcgctgccg gtccacatat tatcctgccg ccgtcacgtc
    15601 actttatcga cgttgagcag gcattttgct tcctgatgta cgcccagacg tttgcactga
    15661 tgcagtcgct gcacatgggc aatacgccgg ataccccatc agccagcggc accgttaacc
    15721 gcgtggtgca aggcgtaatc attcatccgt ggcaggcata agaggatcgc attatgagca
    15781 ttatctccac taaatatctg ttacaggacg cccaggccaa tggctacgcg gtgcctgctt
    15841 ttaacattca taacgccgag acgatccagg cgatcctcga agtgtgcagt gaaatgcgat
    15901 cgccggtgat cctcgccgga acgccgggga cctttaaaca tatcgcgctg gaagagatct
    15961 acgccctgtg cagcgcctat tccacaacct acaacatgcc actggcgctg catctcgacc
    16021 accacgaatc gctggatgat attcgccgta aagtccacgc aggtgtgcgc agtgcaatga
    16081 tcgacggcag ccacttcccg tttgccgaga acgtgaagct ggtgaaatcg gttgttgact
    16141 tctgccactc acaggattgc agcgtggaag cagaactggg ccgcctgggc ggtgttgaag
    16201 atgacatgag cgttgacgcc gaaagtgtat tcctgaccga tccacaagaa gctaaacgct
    16261 ttgtcgaact gactggcgtc gacagcctgg cggtagcgat tggtacggcg cacggcttat
    16321 acagcaaaac gccgaagatt gatttccagc ggctggcgga aattcgtgaa gtggtggatg
    16381 ttcctctggt gctacatggt gccagcgatg ttccggatga atttgtccgt cgcactattg
    16441 aacttggcgt cacaaaagtg aacgttgcca cagaattaaa aatagccttc gctggcgcgg
    16501 ttaaagcctg gtttgcggaa aatccgcagg gtaatgatcc tcgttattat atgcgcgtcg
    16561 gaatggatgc gatgaaagaa gttgtcagaa ataaaattaa tgtctgtggt tcagcgaatc
    16621 gaatttcagc ataatcatta gttttttatg atttatccca atgtacttcc cgattaatat
    16681 cggggagtgc cttaatggaa aaggagatga ctaaacctta ataaatacat cagtacaata
    16741 tcgcaacaat aatatattaa aaaattatat tattcaactt tatggtgagg attacacaat
    16801 gagcagtcca aatattctct taacccgtat tgataaccgt ctggttcacg gtcaggttgg
    16861 cgtgacctgg acatccacca tcggtgcgaa tctgctggta gtcgtggatg atgttgtcgc
    16921 taacgatgat attcaacaga aattaatggg tattaccgca gaaacctacg gctttggcat
    16981 tcgtttcttt actatcgaaa aaaccattaa cgtcatcggc aaagctgcac cgcatcagaa
    17041 gatcttcctg atttgccgta cgccacaaac ggtacgtaaa ttggtagaag gtggcattga
    17101 cctgaaagat atcaacgtcg gcaatatgca tttctcggaa gggaaaaagc aaatcagcag
    17161 taaagtttat gtcgatgacc aggatctcgc ggacttacgt tttattaaac aacgtggcgt
    17221 gaatgttttc attcaggacg tccctggcga tcaaaaagaa caaatccctg actaaatcta
    17281 aaatcgcctt aatattggtt tgaggtaata aaaatgcatg aaataaccct acttcaggga
    17341 ttatccctgg cggcgttagt tttcgttctg gggattgatt tttggctgga agccttattt
    17401 ttattccgcc cgataatcgt ttgtacccta actggcgcta ttctcggtga tattcagact
    17461 ggcttaatta ccggtggtct gacagagttg gctttcgccg gattaacccc tgcaggtggt
    17521 gttcagccgc ccaacccgat tatggcgggt ctgatgacca ccgtcattgc atggtctacg
    17581 ggcgttgatg ccaaaacagc aattggtctt ggcctgccgt ttagtttgtt aatgcagtac
    17641 gtcattctgt tcttctattc cgctttctca ttatttatga ccaaagccga taaatgcgcg
    17701 aaagaggcgg atacggcagc gttttcccag cttaactgga caacgatgct catcgtcgct
    17761 tcagcgtatg cggtgattgc tttcctctgt acttacctgg cacaaggggc gatgcaggcg
    17821 ctggtgaaag cgatgcccgc ctggttgacc cacggctttg aagtggctgg cggtattctg
    17881 cctgccgttg gttttggctt gctgctgcgc gtaatgttca aagcgcaata tatcccttac
    17941 ctgatcgccg gtttcctgtt tgtttgctac atccaggtca gcaacctgtt gccggttgcc
    18001 gtactgggcg caggctttgc ggtgtatgag tttttcaatg cgaaatcccg gcagcaagcg
    18061 caaccgcagc ccgttgccag taaaaatgaa gaagaggact acagcaatgg gatctgaaat
    18121 cagtaaaaaa gatatcaccc gtctgggctt tcgttcgtcg ctgctgcaag cgagctttaa
    18181 ctacgaaagg atgcaggcgg gcggttttac ctgggcgatg ttgccgatcc tgaaaaagat
    18241 ttataaggac gacaaaccgg gcttaagcgc ggcgatgaaa gataacctcg aatttattaa
    18301 tacccacccg aatctggtcg gattcctgat ggggttatta atttcgatgg aagaaaaagg
    18361 agaaaaccgc gacaccatta aaggcctcaa agtggcactg tttggcccaa tcgccgggat
    18421 tggcgatgcg attttctggt ttactttgtt gccgattatg gcgggaattt gctcatcatt
    18481 tgccagccag ggaaacctgc tggggccgat tctatttttc gccgtttacc tgcttatctt
    18541 tttcctgcgc gtcggctgga cccacgtcgg ttattcagtc ggcgtgaagg cgatcgataa
    18601 agtgcgagag aactcgcaga tgattgctcg ctcggcaacc atcctcggga tcacggtaat
    18661 cggcgggctg atcgcttcgt atgtgcatat taacgtggtg acatcgtttg ccatcgacag
    18721 tacccacagc gccgcgctgc aacaggattt cttcgataaa gtcttcccga acattttacc
    18781 gatggcctac accctgctga tgtattactt cctgcgggtg aaaaaagcgc atccggtgct
    18841 gttaatcggc gtgacttttg tgctctctat tgtttgttcc gcattcggca ttttgtaaat
    18901 ggaacgaggc actgcttctg gcggtgcctc tttacttaag gaatttcatc ctgtgcaaac
    18961 ccttcagcaa gttgaaaact atacggcgtt aagtgaacga gccagcgaat atttattggc
    19021 tgtgatccgt agcaaaccgg atgccgtgat ttgcctggcg accggagcca cgccattact
    19081 gacgtatcat tatctggtag aaaaaatcca ccagcagcag gttgatatca gccagctcac
    19141 cttcgtgaag ctcgacgaat gggtggatct gccattaacg atgcctggca cctgcgaaac
    19201 tttcctacaa cagcatatcg tgcagccgct ggggctgcgt gaagaccagc tcatcagctt
    19261 tcgctccgaa gagataaatg agacagagtg cgaacgggta acgaacctga ttgcgcgcaa
    19321 aggcggtctg gatttatgcg ttctcgggtt ggggaaaaac ggtcatcttg ggctgaacga
    19381 accgggagaa agcctgcaac cggcctgcca tatcagtcaa cttgatgcca gaacacagca
    19441 acatgagatg ttaaaaaccg cgggccatcc cgtgactcgt gggatcacct taggcctgaa
    19501 ggatattctc aatgcccgcg aagttttgtt attggtgact ggcgaaggaa agcaggatgc
    19561 gacagaatgt tttctcacgg ctaaagtctc taccgctatc ccggcttcat ttttatggct
    19621 gcacagtaac tttatttgtt taattaatac ctaaccagat gaaacatttg ctcaaaatgt
    19681 tt
//
LOCUS       DAZKHC020000077        19623 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZKHC020000077 DAZKHC020000000
VERSION     DAZKHC020000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.077"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(212..1861)
                     /gene="pgi"
                     /locus_tag="ACHOSM_RS16755"
                     /old_locus_tag="pgaptmp_003351"
     CDS             complement(212..1861)
                     /gene="pgi"
                     /locus_tag="ACHOSM_RS16755"
                     /old_locus_tag="pgaptmp_003351"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGU4559802.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2098..2294
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2386..3735
                     /gene="lysC"
                     /locus_tag="ACHOSM_RS16760"
                     /old_locus_tag="pgaptmp_003352"
     CDS             2386..3735
                     /gene="lysC"
                     /locus_tag="ACHOSM_RS16760"
                     /old_locus_tag="pgaptmp_003352"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HGU4559803.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIGRLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            3987..4259
                     /locus_tag="ACHOSM_RS16765"
                     /old_locus_tag="pgaptmp_003353"
     CDS             3987..4259
                     /locus_tag="ACHOSM_RS16765"
                     /old_locus_tag="pgaptmp_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HGU4559804.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4392..5263)
                     /gene="rluF"
                     /locus_tag="ACHOSM_RS16770"
                     /old_locus_tag="pgaptmp_003354"
                     /pseudo
     CDS             complement(4392..5263)
                     /gene="rluF"
                     /locus_tag="ACHOSM_RS16770"
                     /old_locus_tag="pgaptmp_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
     gene            5611..6558
                     /locus_tag="ACHOSM_RS16775"
                     /old_locus_tag="pgaptmp_003355"
     CDS             5611..6558
                     /locus_tag="ACHOSM_RS16775"
                     /old_locus_tag="pgaptmp_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGU4559805.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDKETQLAMMGLHGAQL
                     LDRLLESGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISTYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            6628..7434
                     /locus_tag="ACHOSM_RS16780"
                     /old_locus_tag="pgaptmp_003356"
     CDS             6628..7434
                     /locus_tag="ACHOSM_RS16780"
                     /old_locus_tag="pgaptmp_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4559806.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQDANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7444..7851
                     /locus_tag="ACHOSM_RS16785"
                     /old_locus_tag="pgaptmp_003357"
     CDS             7444..7851
                     /locus_tag="ACHOSM_RS16785"
                     /old_locus_tag="pgaptmp_003357"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HGU4559807.1"
                     /translation="MVNAIFCAHGKLAFAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            7851..8345
                     /locus_tag="ACHOSM_RS16790"
                     /old_locus_tag="pgaptmp_003358"
     CDS             7851..8345
                     /locus_tag="ACHOSM_RS16790"
                     /old_locus_tag="pgaptmp_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGU4559808.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8411..9208
                     /locus_tag="ACHOSM_RS16795"
                     /old_locus_tag="pgaptmp_003359"
     CDS             8411..9208
                     /locus_tag="ACHOSM_RS16795"
                     /old_locus_tag="pgaptmp_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HGU4559809.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIIGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            9219..10043
                     /locus_tag="ACHOSM_RS16800"
                     /old_locus_tag="pgaptmp_003360"
     CDS             9219..10043
                     /locus_tag="ACHOSM_RS16800"
                     /old_locus_tag="pgaptmp_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGU4559810.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            10095..11324
                     /gene="sorE"
                     /locus_tag="ACHOSM_RS16805"
                     /old_locus_tag="pgaptmp_003361"
     CDS             10095..11324
                     /gene="sorE"
                     /locus_tag="ACHOSM_RS16805"
                     /old_locus_tag="pgaptmp_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HGU4559811.1"
                     /translation="MKTTALRLYGKRDLRLETFDLSEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            11707..12396
                     /gene="pepE"
                     /locus_tag="ACHOSM_RS16810"
                     /old_locus_tag="pgaptmp_003362"
     CDS             11707..12396
                     /gene="pepE"
                     /locus_tag="ACHOSM_RS16810"
                     /old_locus_tag="pgaptmp_003362"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HGU4559812.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12487..14118)
                     /locus_tag="ACHOSM_RS16815"
                     /old_locus_tag="pgaptmp_003363"
     CDS             complement(12487..14118)
                     /locus_tag="ACHOSM_RS16815"
                     /old_locus_tag="pgaptmp_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGU4559813.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14338..18021)
                     /gene="metH"
                     /locus_tag="ACHOSM_RS16820"
                     /old_locus_tag="pgaptmp_003364"
     CDS             complement(14338..18021)
                     /gene="metH"
                     /locus_tag="ACHOSM_RS16820"
                     /old_locus_tag="pgaptmp_003364"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HGU4559814.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWGVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKMGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18221..19045
                     /gene="iclR"
                     /locus_tag="ACHOSM_RS16825"
                     /old_locus_tag="pgaptmp_003365"
     CDS             18221..19045
                     /gene="iclR"
                     /locus_tag="ACHOSM_RS16825"
                     /old_locus_tag="pgaptmp_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HGU4559815.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            19362..>19623
                     /locus_tag="ACHOSM_RS16830"
                     /old_locus_tag="pgaptmp_003366"
     CDS             19362..>19623
                     /locus_tag="ACHOSM_RS16830"
                     /old_locus_tag="pgaptmp_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559816.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKDFVIKFSTSVLQADKKEITII"
BASE COUNT         4678 a         5094 c         5086 g         4765 t
ORIGIN      
        1 agacaagggg atttatctga taaaaaaatg ccgacataac gtcggcattg ttattaaggt
       61 tatttaatca atcgtgctat gtattaattg ccgaatacga tgtacatatc ggcatcgacc
      121 tgtaggcctg ataagacgcg accgcgtcgc atcaggcatc ggttgccgga tgcggcgtga
      181 acgccttatc cggcctacat atcgacgatg attaaccgcg ccacgcttta tagcggttaa
      241 tcagaccatt ggtcgagcta tcgtggctgc tgatttcttt atcatctttc agctctggca
      301 gaatacggtt cgccagttgt ttacccagct ccacgcccca ctggtcgaag gtgaagatgt
      361 tcagaatcac gccctgagta aagattttgt gctcatacag cgcaatcaac gcacccaggc
      421 tgaacggagt gatttcgcgc agcaggatgg agttggtcgg gcggttacct tcgaacactt
      481 tgaacggcac cacgtagtca agcgttgccg gatctttacc ctgatcacga tattcctgct
      541 caaccacttc gcgggattta ccaaacgcca gcgcttcggt ctgagcaaag aagttagaca
      601 gcagtttctg gtgatgatca gagagcgggt tatgggtgat agccggagcg atgaaatcgc
      661 acggtaccat tttggttccc tggtggatca gctggtagaa cgcgtgctga ccgttagtgc
      721 ctggttcacc ccagataatc gggccagtct ggtaatccac aacgttaccg ttacggtcaa
      781 catacttacc gttggactcc atattgccct gctggaagta cgccgcgaaa cggtgcatat
      841 actggtcata cggcagaatc gcttcagttt ccgcaccaaa gaaattgttg taccagatgc
      901 caatcagcgc cagcaggaca ggcaggtttt tctcggcagg cgtggtggag aaatgcttgt
      961 ccattgcgtg tgcgccagaa agcagttcaa cgaagttatc aaagccgatg gagagaacaa
     1021 tcgacaggcc aatcgctgac cacaaagagt aacggccgcc gacccagtcc cagaactcga
     1081 acatgttggc agtatcaata ccaaactcac caacggcttt ggcattggtg gaaagcgccg
     1141 caaagtgttt tgcaacgtgt ttttcatcac ctgccgcttt caggaaccag tcacgcgcgc
     1201 tatgggcgtt ggtcatagtt tcctgagtgg tgaaggtttt agatgctacc aggaacagcg
     1261 tggtttccgg gtttactttt ttcagcactt cagcgatgtg agtcccatcg acgttagaaa
     1321 caaagtgcat gttcaggtga tttttgtacg gacgcagtgc ttcggtcacc atgtacgggc
     1381 cgaggtcaga accaccgatc ccgatgttca ctacgtcagt gattgctttg ccggtataac
     1441 ccttccactc accggagata atcgcttccg agaaggtttt catcttctcc agcaccgcgt
     1501 tgacttccgg cattacgtct ttgccatcaa ccagaatcgg ggtattgcta cggttacgca
     1561 gcgctacgtg cagcacggcg cggttttcag tgcggttgat cttctcgcca gagaacatcg
     1621 acttaatcgc gcccgccaga tcgcactctt tcgccagatc ctgtaatttc gccagcgtct
     1681 cttcagtgat gcggtttttg gagtaatcca ccagcatctg atcgtcgaat gttgcggaga
     1741 acttagaaaa acgatcgccg tctttagcaa aaagatcggc gatcgtaacg tctttcattt
     1801 catcgaagtg tttctgtagt gcctgccagg cagcggtctg cgttggattg atgtttttca
     1861 ttagcaatac tcttctgatt ttgagaattg tgactttgga agattgtagc gccagtcaca
     1921 gaaaaatgtg atggttttag tgccgttagc gtaatgttga gtgtaaaccc ttagcgcagt
     1981 gaagcattta ttagctgaac tactgaccgc caggagtgga tgaaaaatcc gcatgacccc
     2041 gtcgttgaca accgccccgc tcacccttta tttataaatg tactacctgc gctagcgcag
     2101 gccagaagag gcgcgttgcc caagtaacgg tgttggagga gccagtcctg tgataacacc
     2161 tgagggggtg catcgccgag gtgattgaac ggctggccac gttcatcatc ggctacaggg
     2221 gctgaatccc ctgggttgtc accagaagcg ttcgcagtcg ggcgtttcgc aagtggtgga
     2281 gcacttctgg gtgaaaatag tagcgaagta tcgctctgcg cccacccgtc ttccgctctt
     2341 cccttgtgcc aaggctgaaa atggatcccc tgacacgagg tagttatgtc tgaaattgtt
     2401 gtctccaaat ttggcggtac cagcgtagct gattttgacg ccatgaaccg cagcgctgat
     2461 attgtgcttt ctgatgccaa cgtgcgttta gtagtcctct cggcttctgc tggtatcact
     2521 aatctgctgg tcgctttagc ggaagggctg gaacctggcg agcgattcga aaaactcgac
     2581 gctattcgca acatccagtt tgccattctg gaacgtctgc gttacccgaa cgttatccgt
     2641 gaagagattg gacgtctgct ggagaacatt actgttctgg cagaagcggc ggcgctggca
     2701 acgtctccgg cgctgacaga tgagctggtc agccacggcg agctgatgtc gaccctgctg
     2761 tttgtcgaaa tcctgcgcga acgcgatgtt caggcacagt ggtttgatgt acgtaaagtg
     2821 atgcgtacca acgaccgatt tggtcgtgca gagccagatg tagccgcgct ggcggaactg
     2881 gccgcgctgc agctgctccc acgcctcaat gacggcttag tgatcaccca gggatttatc
     2941 ggtagcgaaa ataaaggtcg tacaacgacg cttggccgtg gaggcagcga ttatacggca
     3001 gccttgctgg cggaggcttt acacgcatct cgtgttgata tctggaccga cgtcccgggc
     3061 atctacacca ccgatccacg cgtagtttcc gcagcaaaac gcattgatga aatcgcgttt
     3121 gccgaagcgg cagagatggc aacttttggt gcaaaagtac tgcatccggc aacgttgcta
     3181 cccgcagtac gcagcgatat cccggtcttt gtcggctcca gcaaagaccc acgcgcaggt
     3241 ggtacgctgg tgtgcaataa aactgaaaat ccgccgctgt tccgcgcgct ggcgcttcgt
     3301 cgcaatcaga ctctgctcac tttgcacagc ctgaatatgc tgcattctcg cggcttcctc
     3361 gcggaagttt tcggcatcct cgcgcggcat aatatttcgg tagacttaat caccacgtca
     3421 gaagtgagcg tggcattaac ccttgatacc accggttcaa cctccactgg cgatacgttg
     3481 ctgacgcaat ctctgctgat ggagctttcc gcactgtgcc gggtggaagt agaagaaggt
     3541 ctggcgctgg tcgcgttgat tggcaatgac ctgtcaaaag cctgcggcgt tggcaaagag
     3601 gtattcggcg tactggaacc gttcaacatt cgcatgattt gttacggcgc atccagccat
     3661 aacctgtgct tcctggtgcc cggcgaagat gccgagcagg tggtgcaaaa actgcatagt
     3721 aatttgtttg agtaaatact gtatggcctg gaagctatat ttcgggccgt attgattttc
     3781 ttgtcactat gcttatcaat aaacgagcct gtactctgtt aaccaacgtc tttatcgaga
     3841 ataattgcct ttaatttttt tatctgcatc tctaattaat tatcgaaaga gataaatagt
     3901 taagagaagg caaaatgaat attatcagtt ctgctcgcaa acgaattccc gtacactgaa
     3961 ctctctggct cactgagggc accatcatgg cactcccccg tattacccaa aaagagatga
     4021 ctgaacgcga gcagcgtgaa cttaaaacac tgctggatcg ggcgcggatt gcgcatggtc
     4081 gcgtactgac caactccgaa accaacagca ttaagaaaga gtacatcgat aaattaatgg
     4141 tcgagcgtga ggctgaagcg aaaaaagccc gccaattgaa gaaaaagcag gcttataaac
     4201 cggatcctga agcgtcattt agctggtcag caaatacgtc gacgcgcggc aggcgctaat
     4261 gaacaatcaa cgcctgagat tttcactcgt ctgtgtggac acaatcttcc cctcacccta
     4321 accctctccc cgaaggggcg aggggactgt ccgagctctt tttggacgtt ttcatcagtc
     4381 tgaggcgtta atcagcgtcc cttcttttta cgccccggcg aggtaaaacg cttaccgttg
     4441 gacgccgggc gaccgccttt ttccgccgtt tttccatctt aacgactgga cgtttgatgc
     4501 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
     4561 gcttaaagag gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc
     4621 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
     4681 gctcgcacat gcgccggatc tgacggttca gcccctgcac cagggtaatg cggaagacaa
     4741 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
     4801 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
     4861 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
     4921 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
     4981 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
     5041 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     5101 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     5161 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     5221 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     5281 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     5341 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     5401 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     5461 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     5521 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     5581 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     5641 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     5701 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatt
     5761 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     5821 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga taaagagact
     5881 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagtctggc
     5941 gatattgtcg gtttttcttg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     6001 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     6061 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     6121 tcgcatctcg cggattttcc ggctctgctg gataacccct taattcgtaa tgggatcatg
     6181 cagtctcagc actttaaaac catctctacc tactgggata atctggatgt cgccctggtg
     6241 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     6301 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     6361 attcacggcg caatggttga aacgaatatg agcgaaaaaa cactctctat cgaaatgaat
     6421 aaattaaagc aggcacgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     6481 attgttggtg cattgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     6541 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     6601 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     6661 attatcgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     6721 caagacgcaa acgtacagat ggtagatatt cacggtggcg atggtcaata tgaaggtcat
     6781 aaaggctatc agttttggcc gaccgatatt tccagcgcca aagaggtaaa tcatacggta
     6841 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     6901 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     6961 gcattcgaaa aaatggtcaa tatcaaccag aaaggcgttt ttctgatgtc gcaggcggtg
     7021 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtgga
     7081 ctggaaggct cagaaggcca aagctgttac gccgctacca aagccgcgct caatagcttc
     7141 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     7201 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     7261 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     7321 cgcgccggaa gattagcaga agtcgctgat tttgtttgtt atctgctgtc tgaacgcgcc
     7381 agctatatca ccggagtaac cactaacatt gcaggcggca aaacgcgcgg ctaaggaggc
     7441 tttatggtca acgccatttt ttgtgcccac ggcaaattag ctttcgccat gctggaatcg
     7501 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg aatttgtgcc cggcgagaac
     7561 gccggagaca tcgtagcaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg
     7621 ctgattgccg tggatttaca gtgcggtagc ccgtggaatg cggcagcgat gcttgcgatg
     7681 cgcaatcctc gtctgcgggt aatcagcggc ctttccctgc cgctggcgct cgaactggtt
     7741 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     7801 caaacctgcg ttgtctggaa acaactggca acggcagagg aggatttctg atgaacatta
     7861 cgctcgctcg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     7921 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     7981 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     8041 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     8101 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     8161 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     8221 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     8281 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     8341 attaaataat agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag
     8401 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     8461 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     8521 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     8581 attgatagct ctcggctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     8641 cagcataatc tccgccattc tggttatcat tggtcagcaa agcatcgcca ccggaatcgc
     8701 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgcac gtaccatcac
     8761 cgtagtgttc cagcacgcgg cggataaagc agcggaagaa gcacggtttc gcactctcga
     8821 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gttgctattc cggcactgat
     8881 tgtctcgctg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     8941 tgtgacccga ggtctgcaaa ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     9001 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     9061 tggctacctc gatctcagtc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     9121 ctacatccag ctaaatccac agtggcgtaa agctgaacct caaccccagg ccaccacctc
     9181 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     9241 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     9301 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     9361 cattaaaaga ggatcaggtt gcggcgctca ggcgacacct agtgttcttc aataccacgc
     9421 cagccgtatg tggcccggtc atcggcgtca ctgccgccat ggaagaggcg cgggccaacg
     9481 gcgcggaaat tgatgacggt accattaacg gcatcaaagt cggtctgatg ggaccattgg
     9541 caggggttgg cgatccactg gtttggggaa cgctgcgccc gattaccgct gcgctcggcg
     9601 catctctggc actttctggc aacatcctcg gcccgctttt gtttttcttt attttcaatg
     9661 cggtgcgtct ggcgatgaag tggtatggcc tacagctcgg ctttcgcaaa ggagtgaata
     9721 tcgtcagcga tatgggcggg aatgtgctgc aaaaactcac cgaaggcgcg tcgattctcg
     9781 gactgtttgt gatgggcgtg ctggtcacca aatggacgtc aatcaacgta ccgttggtgg
     9841 tttcacaaac gcatgccgcc gatggctcca ccgtcaccat gaccgtgcag aacattctcg
     9901 accaactttg ccctggtttg ctggcgctcg gtctgacgct gcttatggtt cgtctgctca
     9961 acaaaaaaat taacccggta tggctgattt tcgccctgtt tggcttaggg attatcggta
    10021 atgcgctggg cttcctgtcc tgattcttcg ccccggcacg actgccgggg ccatcgctca
    10081 acatgaggtg gtttatgaaa acaacagctc tgcgtcttta tggtaaacgt gatttacgcc
    10141 tggaaacctt tgacctttct gaaatgcagg aggatgaaat cctcgcgacg gtggtcactg
    10201 acagcctgtg cctctcttcc tggaaagagg ccaatctggg tgaaaaccat aaaaaagtac
    10261 ccgacgatgt ggcgaccaac cccatcatca tcggccacga gttttgcggc gatattctgg
    10321 ccgtgggtaa aaagtggcag cacaaattcc agccgggtca gcgttatgtg attcaggcca
    10381 acctgcaact ccccgaccgc ccggactgcc ccggctactc cttcccgtgg gtgggcggcg
    10441 aagccacgca tgtggttatt cccaacgagg tcatggaaca agattgcctg ctggcatacg
    10501 acggcgaaac ctattttgaa ggctcactgg ttgaaccgct ttcctgcgtg attggcgcgt
    10561 tcaacgccaa ctatcatctt caggaaggta gttataacca cacgatgggg attcgcccgc
    10621 aagggcgcat gctgatcctc ggcggcaccg gaccaatggg actgttggcg attgattatg
    10681 cgctacatgg acccgttaac ccgtcgctgc tcgtcattac cgataccgac aacgataaat
    10741 tgagttatgc gcgcaagcac tatccatcag aaccgcaaac actgattcat tatctcaatg
    10801 ccgccgatgc agcatttgat acgctaatgg cgctgagtgg cggtcacggc ttcgatgata
    10861 ttttcgtctt tgtgcctaat gaaggactgg tgactctcgc ctcttccttg ttggcgacag
    10921 atggttgcct gaatttcttc gccggaccgc aggataaaca tttcagcgcg ccaattaatt
    10981 tctacgatgt gcattatgca tttacccact acgtgggcac gtcaggcggc aataccgacg
    11041 acatgcgcgc ggcggtcaaa ctgattgaag agaaaaaagt gcaggccgca aaagtggtaa
    11101 cacatattct tgggctgaat gccgcgggcg aaaccacgct tgaattgcct gccgtcggcg
    11161 gcggcaaaaa gctggtgtat accgggaaat acctgccgct gacgtcactc acgcagattc
    11221 aggatcaagc actggcggcg attctggcgc gtcatcaggg gatctggtcc ggtgaggcgg
    11281 agcaatatct gctcactcat gcagaggcaa tttcccatga ttaatcgcgc tacttaactc
    11341 tgcatgtcgc tggctggtcg ccgcggcaac ttcgcgacct gatgtgcggc tgattgctga
    11401 agcggcccaa tgtgcacgag aaggataaaa aaacatcagc gccttaaggg gcgctcaaat
    11461 tggtagcaaa gtacacattg tttatgccgg atgcggaata aagccttatt cggcctacat
    11521 aatctcgaaa attcaatata ttgcagttat ctggtaggcc tggtaagcgt agcgcatcag
    11581 gcaatgctgt gtttgtcatc agtctcagcg cccggtgcaa tacgtgacat cgtgagccat
    11641 gtcagtaagt ggttaaattc gcagtggtag catgccttcc gtgatttctt actaaccgga
    11701 gaagtaatgg aactgctttt attgagtaac tcgacgctgc cgggtaaagc ctggctggaa
    11761 catgcactgc cgctaattgc tgaacagttg cagggtcgcc gctcagcggt gtttattcct
    11821 ttcgctggcg taacgcagac ctgggatgat tacacagcga aaacggctgc ggttctcgct
    11881 ccgctgggtg tatctgtcac cggtattcat agcgttgtcg atcccgttgc cgcgattgaa
    11941 aatgctgaga tcgtgattgt cgggggcggg aatactttcc agttgctgaa acagtgccgc
    12001 gagcgcgggc tgctggcacc aattactgac gtggttaaac gtggtgcgct gtatattggc
    12061 tggagcgcag gcgctaacct tgcttgccca accattcgta ccaccaacga tatgccgatt
    12121 gtcgatccgc aaggtttcga tgcgctaaat ctgttcccgc tgcaaatcaa cccgcacttc
    12181 accaacgcgc tgccggaagg ccataaaggt gaaacccgtg agcagcgtat tcgcgaactg
    12241 ctggtcgtcg cgccagaact gacgattatt ggtctaccgg aaggtaactg gatcacagtg
    12301 agtaaaggtc acgctacgct gggtggcccg aacaccactt atgtgtttaa ggctggtgaa
    12361 gaagcggttc cgctggaagc tggtcaccgt ttttaagtga attgccggat gcatgatccg
    12421 gcatcttgtt catagtgcct gatgcgacgc ttgacgcgtc ttatcaggcc tacaggattc
    12481 acaatattaa tactcgtccc gtccttcatc ttcatccggc tgttccagca cactgtaagc
    12541 caccgaacaa aacagcgagt tcaggcgctg catatctccc agtaagccta aatgtagcga
    12601 actggtttca atgctctgca cgttttgctg atgcaggcga tcgacgtggg cgtgggaata
    12661 gcggcgatta agaatgcgaa aacgatgttt gctgcgacgc aaacgacgag cgctggtgac
    12721 atcgccagag aagaacaccg acattgccag ctttaaatta ctgagcaatt gctcatagag
    12781 cgcatccagt tccttcaacc catcaagcga aaatgcccgc cgtgctgcca gcgatttatc
    12841 ggcaatttcg ctgcccatgc gctcgacgat atcggaggcc tgttcaaggt tgagcgacat
    12901 ttcgatgatc tccgcccagc ggcgcgactc ttcctccgcc aactcttctt ttggcatccg
    12961 cgccagatac agcttaatgg cggtgtagag aacgttgata tcatctgcca gcttacgcag
    13021 ctctttctcc tgccgtggct cgccgtgcat cactttattt agcccttcca tcatctgttc
    13081 catggcgtcg ccaatgcgca gggtttcgcg cgcggcgttc gccagagcaa gcgtgggcgt
    13141 atccagcgcg ctgacatcca gatgtttggg ccgtagctgg gtatccagtt ccggttcatc
    13201 gcgaataatc gttttgcaaa accgtgccat cgggtcaaca aatggcagca tgaccaggca
    13261 acgtacaagg ttgtagaaga cgtggaaata gatcaccagt tccgcttttg gcaatgacaa
    13321 cttccccatt gtctctgcca gcaaatggac aaacggcagg ataatcaggc tacccaccag
    13381 cttaaacagc agactaccca gcgcgacacg gcgggctgcg gcattggcgg cactgttgtt
    13441 gagcatcgcc agcagaccgg aaccgaggtt agcaccaatc accagacaga gcgccacggg
    13501 gaaggagata atgcctgcgg cggtcagagt cgcggtcagc agtacagcag caaggctgga
    13561 gtagctgata atggcgaaca tcgcgccaat cagcgcatcc agcagaatat cgccggtcag
    13621 cgaggcaaag atcacctgaa cgccgtttgc ctgggtgatc ggcgttacgg cctgcacaat
    13681 caactccagc gctagcaaaa tcagcccaag accaataccg acgcggccca gttgcccggc
    13741 gcgtgactgt ttgcgtccga ggaaaaaaat cacgccgata aaaataagta acggtgacag
    13801 ccaggataag tcgaaggtga gaatacgcgc cattagcgcc gtcccgacat ctgcacccag
    13861 cacaatgacc agagccggtg cgagggctac cagatcctga gcgacaaacg aggtcaccag
    13921 catggtggtg gcattactgc tctgtaccag tgcggtaacg ccgatccccg cgcaaaaggc
    13981 gagcggcttt ttttcgacgc tccggctaag gacagtacgc aaacgcgcgc cgaagacgcg
    14041 cattacgccg gttcgaacaa tatgagtccc ccagaccagc agggcgacgg cagaaagcag
    14101 gtgaagcagc gttaacacga gttcaggttc tccttatcgt tatatgttcc tgaagcaatt
    14161 gaatgaccaa aggaccggaa acacgctgac tggcatcttt actgtgcctt ttgacattcg
    14221 tttttccggc taagattatt ccagcctgtt tccagtataa gtgtttcata ttaataaaga
    14281 gacagggcac tcattgagcg ccctgtttgt tgtaaggaaa agtgacagat ttgtgaatca
    14341 gtccgcgtca taccccagat tcggtgccag ccagcgctca acatctgaga cgctcatacc
    14401 tttacggcgg gcatagtctt caacctgatc gcgctgaatt tgcgccacgg cgtagtactt
    14461 gctgtccggg tggctgaaat accaacccga aaccgatgca ccgggccaca tggcgaaaga
    14521 ttctgtgagt ttcatgccgg tatgtttttc cacttccagc agctcccaga tggtggcttt
    14581 ttccgtatgt tccgggcagg ccgggtagcc cggtgccgga cggatgccct ggtagttttc
    14641 gcggatcagt tcttcgttgc tgaggttctc gttcggcgca tagccccagt agactttacg
    14701 cacacgctca tggagatact ccgcaaaggc ttcggctaaa cggtcggcaa gcgctttcac
    14761 catgattttg ttgtaatcat cgtgctgcgc ttcaaaggca tcagccagtg cgtcctcttc
    14821 cagcccgcca gtcacggcaa atgcgccgat gtaatctgct ttaccagaaa gcttcggcgc
    14881 aacgaagtca gcgagacagt agttagcgaa tcccattttt tcggtctgtt gacgcagatg
    14941 gtggctgacg ttgatcacat gggtgcgcgt ttcgtcacgg tagatttcaa tgtcatcgcc
    15001 cacacggttt gccgggaaca ggcccaccac gccacgcgga ttcagcgttt tctcggcgct
    15061 taatttatcc agcatgtcgt tggcgtcttt aaacagccgc tgcgcctcaa cgcccaccac
    15121 ttcatcttcc agaatgcgcg gatacttccc ggccagcgac caggtcataa agaacggtgt
    15181 ccagtcgatg taattacgca gagtttcgat gctggcttca acttcctgca cgccgagacg
    15241 gtgcgccacc ggcggcgtgt aagcctgcca gtcaaaagcg aagtcgttat cgcgcgccgc
    15301 ttccagcgtg accggtggtg tgcgcggttt cttgcgcccg tgctgaatac gtacggtttc
    15361 gtactccttg cgggtacgag cgacaaaatc atcgcgctga gtatcggaaa gcagcgccgc
    15421 caccacacca acggtgcgcg aggcattctg cacatacacc gtcgggccgc tgtagttctg
    15481 ctcgattttc accgccgtgt gcgcttttga ggtcgtcgcg ccgccaatca atagcggaat
    15541 agtgaagccc tgacgctcca tctctttcgc cacgttaacc atctcgtcca gcgacggcgt
    15601 gataagcccc gaaaggccaa tcaaatcagc attcacttct ttggcggtac ggagaatttt
    15661 ttccgcaggc accatcacac cgagatcgac aatttcgtag ttgttacatt gcagcaccac
    15721 gccaacgata tttttaccga tgtcgtggac atcgcctttc acggtggcga tcaccatctt
    15781 gccgttggtt ttaccctgct ctttgctggc ttcaataaac ggttcgagat acgccactgc
    15841 ctgtttcatg acgcgcgccg atttgaccac ctgtggcagg aacattttcc cttcgccaaa
    15901 cagatcgccg accacgttca taccgtccat cagcgggcct tcaatcactt caatcgggcg
    15961 cgtagcctgc tggcgggctt cttcggtatc ctgctcgata aactcggtaa tgcctttaac
    16021 cagcgagtat tccagacgtt tattcacttc ccacgagcgc cactccgcct gctgggcgtt
    16081 agcggtgtcg tcggttttgc tgccgcgata tttctcggca agctccagta aacgctcagt
    16141 gccatcgtcg cgacggttaa gaatcacatc ttccaccgcg tcgcgcagtt cagcgggtag
    16201 gtcgtcgtaa atcgccagtt gcccggcgtt gacgatcccc atatccatgc cattgcgaat
    16261 agcgtagtag aggaacaccg cgtgaatggc ttcgcgcacc ggatcgttgc cacggaatga
    16321 gaaagaaacg ttagatacac cgccggaaat cagcgcgtgc ggcagttcgc gtttgatatc
    16381 ttcacacgcg ccgataaagt cctgcgcgta gttgttgtgc tcttcaatgc cggttgcgac
    16441 cgcgaagatg ttcgggtcga agatgatatc ttctggcggg aagccaacct cttcggtgag
    16501 gattttgtac gcccgacggc aaatctcgat tttccgtgcg cgagtatcgg cctgtccctg
    16561 ttcgtcaaag gccattacca ccactgccgc accgtagcga cgcaacaatt tcgcgtgatg
    16621 gataaaggcg tcgacgccct ctttcatcga gattgagtta acaatgcctt tgccctgaat
    16681 acacttcagc cctttttcga taacgcccca ttttgaggag tcgatcataa tcggcacgcg
    16741 agcgatatcc ggttcaccgg caatcagatt gagaaaacgc accatcgccg cttcggcatc
    16801 gagcatccct tcatccatgt tgatatcgat aatctgcgcg ccgttttcca cctgctgacg
    16861 cgccacatcc agcgcctcgc tgtatttctc ttctttgatc aggcgcttga acttagcaga
    16921 gccggtgacg ttggtgcgtt cacccacgtt cacaaacagg ctatcgtcgc caatgttcag
    16981 cggctccagg ccagacaaac ggcaggctac gggaatttcc ggcagtttgc gcggcgctaa
    17041 tccttctact gcacgactca tcgctgcaat atgttgcggc gtggtcccgc agcaaccacc
    17101 gacgatattg agaaagcccg cttgcgccca ttcacgtatc tgttttgcca tcgtgtcggc
    17161 gtcgagatcg tattcaccaa aggcgttagg cagcccggcg ttcgggtggg cggtgacgta
    17221 gcattccgca atccgtgata gttcctgcac gtactggcgc agttcatcgg gccccagcgc
    17281 acagttcagg ccaaaggtta gcgcttcggc gtggcgcaat gagttgtaaa atgcttcggt
    17341 ggtctgcccg gagagcgtgc gcccggaggc gtcggtgatg gtgccggaga tcataatcgg
    17401 cagctcaacg cccagcgctt caaactccgt ttttaccgca aataccgccg ctttggcgtt
    17461 aagggtgtcg aaaacggttt caatcaggat cagatccgca ccaccttcca ccagcgcttt
    17521 ggtggactct cgataagccg ccaccagccc gtcaaaagtg atattacgaa atgccggatc
    17581 atttacgtcc ggggaaatag acgccgtgcg gttggtcggg ccgagaacac cggcaacgta
    17641 gcgcggtttc tctggcgtgc gcgcggtcca ctcatcagca caggcgcgcg ccagtttcgc
    17701 cgccgcaaag ttgatttccg ccgacaggga ttccatctgg taatccgcca tcgcaatggt
    17761 cgtggagttg aaggtgttgg tttcgatgat atccgcgccc gcttcaaagt aggcgttgtg
    17821 gatagcggcg atcacttccg gtttgctgag caccagcagg tcgttgttgc ctttgaggtc
    17881 gcatggccag tcggcaaagc gttcaccacg aaaatcggct tcgttcagtc gatagctctg
    17941 gatcatggtg cccataccgc cgtccaacac cagaatacgt tcatttaact gcgcacgcag
    18001 ttgttccact ttgctgctca cacttgctcc cgacacgctc aacccagatt taataaaaat
    18061 tcaacaaacc atactggcat aaacgcatct gtggtaaaag cgaccaccac gcaacatgag
    18121 atttgttcaa cattaactca tcggatcagt tcagcaacta ttgcattagc taacaataaa
    18181 aatgaaaatg atttccacga tacagaaaaa ggagactgtc atggtcgcac ccattcccgc
    18241 gaaacgcggc agaaaacccg ccgttgccac cgcaccagcg actggacagg ttcagtcttt
    18301 aacgcgtggc ctgaaattac tggagtggat tgccgaatcc aatggcagcg tggcactcac
    18361 agagctggcg caacaagccg ggttacccaa ttccacgacc caccgcctgc taaccacgat
    18421 gcaacagcag ggtttcgtgc gtcaggtcgg cgaactggga cattgggcaa tcggcgcaca
    18481 tgcctttatg gtcggcagca gctttctcca gagccgtaat ttgttagcga ttgttcaccc
    18541 tatcctgcgc aatttaatgg aagagtctgg cgaaacggtc aatatggcgg tgcttgatca
    18601 aagcgatcac gaagcgatta ttatcgacca ggtacagtgt acgcatctga tgcgaatgtc
    18661 cgcgcctatc ggcggtaaat tgccgatgca cgcttccggt gcgggtaaag cctttttagc
    18721 ccaactgagc gaagaacagg tgacgaagct gctgcaccgc aaagggttac atgcctatac
    18781 ccacgcaacg ctggtgtctc ctgtgcattt aaaagaagat ctcgcccaaa cgcgcaaacg
    18841 gggttattca tttgacgatg aggaacatgc actggggcta cgttgccttg cagcgtgtat
    18901 tttcgatgag caccgtgaac cgtttgccgc aatttctatt tccggaccga tttcacgtat
    18961 taccgatgac cgcgtgaccg agtttggcgc gatggtgatt aaagcggcga aggaagtgac
    19021 gctggcgtac ggtggaatgc gctgactttt tctggcgggc agaggcaata ttctgcccat
    19081 catacctgag tggcaataga ataagggtgt ctgttaatcg cattgacgcc aaaataactt
    19141 aatgtcatac acttcacaca cttttattaa ctcagcatta tttttaaaca tcaaaccact
    19201 taattattaa caaaatcgca agatctcatt ccaccgaaac ttttacggct gcattatgag
    19261 attgagcgat ttatgatatt tctttagttg taatcatatt aaattttttc actgttctga
    19321 tagttaaaat ttaagacatc aataaacaat gagatattta aatgattact cgtattcctc
    19381 gtagttcttt ctctgcaaat attaataata cagcccagac aaatgaacac caaaccctga
    19441 gtgaattgtt ttataaagaa ctcgaggata aattttctgg caaggagctg gcgacgcctc
    19501 tattaaaaag cttctcagag aactgtagac aaaatggtcg tcatatcttt agcaacaagg
    19561 attttgtcat taaattttcc acgtccgtct tacaagctga taagaaagaa attacgataa
    19621 tta
//
LOCUS       DAZKHC020000078        19154 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZKHC020000078 DAZKHC020000000
VERSION     DAZKHC020000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.078"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(622..915)
                     /locus_tag="ACHOSM_RS16835"
                     /old_locus_tag="pgaptmp_003367"
     CDS             complement(622..915)
                     /locus_tag="ACHOSM_RS16835"
                     /old_locus_tag="pgaptmp_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4559817.1"
                     /translation="MMTDSVAQKLEERGLWRRAATRWAEVLLHAETDSEREEAARRRA
                     ICISKTRRMPEQFVTFGDVKKAADRTLKEMGINPQDEWRNYSFSDAGDDLALP"
     gene            complement(935..1351)
                     /locus_tag="ACHOSM_RS16840"
                     /old_locus_tag="pgaptmp_003368"
     CDS             complement(935..1351)
                     /locus_tag="ACHOSM_RS16840"
                     /old_locus_tag="pgaptmp_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HGU4559818.1"
                     /translation="MNQQTTQQDQTTRKPSRRIPWQHMTKRQRKNRRRINRLAEYWPE
                     LFGAENIKPLKAGVIHDMLQDAKARELAIGHGVLRGALTSYIHTTRYLKAIVAGGARY
                     GLNGQPCGEVTEEDKSVASELLKRRLEQMKQKNSQQ"
     gene            complement(1368..1817)
                     /locus_tag="ACHOSM_RS16845"
                     /old_locus_tag="pgaptmp_003369"
     CDS             complement(1368..1817)
                     /locus_tag="ACHOSM_RS16845"
                     /old_locus_tag="pgaptmp_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4559819.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGTRGNQPPAGGDDPFSDDIPF"
     gene            complement(1814..2263)
                     /locus_tag="ACHOSM_RS16850"
                     /old_locus_tag="pgaptmp_003370"
     CDS             complement(1814..2263)
                     /locus_tag="ACHOSM_RS16850"
                     /old_locus_tag="pgaptmp_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559820.1"
                     /translation="MTEKHAKKSHSHAIDLTEPESVVRFVSVRGRATLYIPDEHLHHC
                     DKHKLPILIVWKRTVYADVTWLNDSLMLIHRDLFEREEFRRDIEERAGKIYEKYAANS
                     KRAARAITYHFMTLYDLKAEDAEKAACDLFDMTMGIIQEYRNKERRP"
     gene            complement(2596..4377)
                     /locus_tag="ACHOSM_RS16855"
                     /old_locus_tag="pgaptmp_003371"
     CDS             complement(2596..4377)
                     /locus_tag="ACHOSM_RS16855"
                     /old_locus_tag="pgaptmp_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HGU4559821.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWQEQDGRLA
                     GDNVPPVWLGEQQLAELDNLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAVAGVRDA
                     DYYPEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGA
                     SQRGEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQN
                     AIKSAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPP
                     AEGETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSG
                     KSVMAEICTMLAGKANTVSASIKALEDARERALVVGFSLIILPDMTRYAGDGAGIKAI
                     TGGDKVAIDPKHKAPYSTRIPAVVLAVNNSAMSFSDRSGGISRRRVIFNFSEVVPENE
                     RDPMLPEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCG
                     YLMSSVMCDGLLVGNAEIVPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGADMPGAMA
                     EYGREYMKKRTKEGVRSNVILTDESED"
     gene            complement(4554..4745)
                     /locus_tag="ACHOSM_RS16860"
                     /old_locus_tag="pgaptmp_003372"
     CDS             complement(4554..4745)
                     /locus_tag="ACHOSM_RS16860"
                     /old_locus_tag="pgaptmp_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559822.1"
                     /translation="MQQITLAEASLQAQQVEVISRMIERNHEEIDDHDLSVIAGLIKQ
                     LSSNVAVWLMTEEEKRGEQ"
     gene            complement(4848..4997)
                     /locus_tag="ACHOSM_RS16865"
                     /old_locus_tag="pgaptmp_003373"
     CDS             complement(4848..4997)
                     /locus_tag="ACHOSM_RS16865"
                     /old_locus_tag="pgaptmp_003373"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559823.1"
                     /translation="MCDFSDMTVDEAAKSAMQAELICSLMMGNTGGMTEGEIESLLAL
                     IKQLR"
     gene            complement(5001..5186)
                     /locus_tag="ACHOSM_RS16870"
                     /old_locus_tag="pgaptmp_003374"
     CDS             complement(5001..5186)
                     /locus_tag="ACHOSM_RS16870"
                     /old_locus_tag="pgaptmp_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559824.1"
                     /translation="MLKTFRVFSRAVNKRGHTIGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            complement(5179..5349)
                     /locus_tag="ACHOSM_RS16875"
                     /old_locus_tag="pgaptmp_003375"
     CDS             complement(5179..5349)
                     /locus_tag="ACHOSM_RS16875"
                     /old_locus_tag="pgaptmp_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4559825.1"
                     /translation="MAVSARPYFVWRFMQCLTDSIAIFTVTAATEREARARLPHAHLI
                     FVARIRQEVTHA"
     gene            complement(5404..5487)
                     /locus_tag="ACHOSM_RS16880"
                     /old_locus_tag="pgaptmp_003376"
                     /pseudo
     CDS             complement(5404..5487)
                     /locus_tag="ACHOSM_RS16880"
                     /old_locus_tag="pgaptmp_003376"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5821..6009
                     /locus_tag="ACHOSM_RS16885"
                     /old_locus_tag="pgaptmp_003377"
     CDS             5821..6009
                     /locus_tag="ACHOSM_RS16885"
                     /old_locus_tag="pgaptmp_003377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559826.1"
                     /translation="MTNEELQEIALSILERQREKAQRQRRLLLNPLPLTRPTRFALED
                     FVEKYPRRLKATKNRPPG"
     gene            complement(6316..7164)
                     /locus_tag="ACHOSM_RS16890"
                     /old_locus_tag="pgaptmp_003378"
     CDS             complement(6316..7164)
                     /locus_tag="ACHOSM_RS16890"
                     /old_locus_tag="pgaptmp_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HGU4559827.1"
                     /translation="MKRPNMPVQGQGFVQSEELSQQYFAAIIPVSPGIIGGYETGIVS
                     ARALHKALGVGRDFTTWIKGRIEEYGFKKGVDFEVVENLYFDSPKRGSQSVNIEQFDS
                     GWTTKRGGDRRSEDYLISTNMAKELAMVERTEQGRAIRQYFIKCEEELHKAAPERAAA
                     LRRELKARVTVASFFKPMCAALELYRAELGKETHQHHYTTESNMLARIVLGGMTAKQW
                     AQANGIKGKPRDSMSVMQLEHLSYLDQSNITLIELGMTYQQRKAELIRLSQRWLARRM
                     GEHEHA"
     gene            complement(7232..7447)
                     /locus_tag="ACHOSM_RS16895"
                     /old_locus_tag="pgaptmp_003379"
     CDS             complement(7232..7447)
                     /locus_tag="ACHOSM_RS16895"
                     /old_locus_tag="pgaptmp_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4559828.1"
                     /translation="MTANHQEAIPTTGYIRRCNLAKLLDTSVSTIDRWVRIGKLPKPT
                     KLGEKNTVFNAVEINHWLNERRNNTKQ"
     gene            complement(7834..8484)
                     /locus_tag="ACHOSM_RS16900"
                     /old_locus_tag="pgaptmp_003380"
     CDS             complement(7834..8484)
                     /locus_tag="ACHOSM_RS16900"
                     /old_locus_tag="pgaptmp_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559829.1"
                     /translation="MINILKKSPIERLCASVSITPHEMALALADLNPSMRIGDVPQDK
                     FEQVESARTHIARAIRLHSGVKTSKDEPYRAGDIFLASFPFMEAGTPEAIIKAVTDTI
                     DDLRGTKNWEEKALNLGGRDLVRYIKETSRSGRGQHRKLDEEQGNMKMMGLLVLLLVK
                     KSGTTAYIQDGEPNRSAIYRDMEALMKEKGISPKGLAKSTFMQKISAALLAVSQAD"
     gene            complement(8481..9743)
                     /locus_tag="ACHOSM_RS16905"
                     /old_locus_tag="pgaptmp_003381"
     CDS             complement(8481..9743)
                     /locus_tag="ACHOSM_RS16905"
                     /old_locus_tag="pgaptmp_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4559830.1"
                     /translation="MPKLTDIQIRAWIKSGERFEGRSDGGGLYLRYRKEDQIPTWRFR
                     YKFAGKARAMVIGSYADYSLSKARETAKELSARVALGYDVAAEKQERKAEALAKIEAE
                     KKAVRVSELAAEYFERQILPRWKHPDILRRRIDKDINPRIGHMKAEDVKPRHIDEMLK
                     AIVDRGAPTIATDVLRWTRRIFDYGVKRHVLEINPCSAFEVSDAGGKESSRDRWLTRE
                     ELIRFFQAMRTAKGFSRENELTFKLLLALCVRKMELCAARWEEFDLDNAVWHLPEERS
                     KNGDPIDIPLVHPVIEWLRELRVLSCNSAWVLPARKMQHRMIPHIQESTLPVALAKVR
                     AEMPEVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRYQYFNERRD
                     ALAKWTTLLVSLERGEHYNVTPIRRAKS"
     gene            complement(9933..10796)
                     /gene="yicC"
                     /locus_tag="ACHOSM_RS16910"
                     /old_locus_tag="pgaptmp_003382"
     CDS             complement(9933..10796)
                     /gene="yicC"
                     /locus_tag="ACHOSM_RS16910"
                     /old_locus_tag="pgaptmp_003382"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HGU4559831.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNVE"
     gene            10923..11639
                     /gene="rph"
                     /locus_tag="ACHOSM_RS16915"
                     /old_locus_tag="pgaptmp_003383"
     CDS             10923..11639
                     /gene="rph"
                     /locus_tag="ACHOSM_RS16915"
                     /old_locus_tag="pgaptmp_003383"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HGU4559832.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            11705..12346
                     /gene="pyrE"
                     /locus_tag="ACHOSM_RS16920"
                     /old_locus_tag="pgaptmp_003384"
     CDS             11705..12346
                     /gene="pyrE"
                     /locus_tag="ACHOSM_RS16920"
                     /old_locus_tag="pgaptmp_003384"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGU4559833.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(12383..12979)
                     /gene="slmA"
                     /locus_tag="ACHOSM_RS16925"
                     /old_locus_tag="pgaptmp_003385"
     CDS             complement(12383..12979)
                     /gene="slmA"
                     /locus_tag="ACHOSM_RS16925"
                     /old_locus_tag="pgaptmp_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HGU4559834.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(13086..13544)
                     /gene="dut"
                     /locus_tag="ACHOSM_RS16930"
                     /old_locus_tag="pgaptmp_003386"
     CDS             complement(13086..13544)
                     /gene="dut"
                     /locus_tag="ACHOSM_RS16930"
                     /old_locus_tag="pgaptmp_003386"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGU4559835.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIAAPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(13522..14742)
                     /gene="coaBC"
                     /locus_tag="ACHOSM_RS16935"
                     /old_locus_tag="pgaptmp_003387"
     CDS             complement(13522..14742)
                     /gene="coaBC"
                     /locus_tag="ACHOSM_RS16935"
                     /old_locus_tag="pgaptmp_003387"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HGU4559836.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGVLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            14914..15582
                     /gene="radC"
                     /locus_tag="ACHOSM_RS16940"
                     /old_locus_tag="pgaptmp_003388"
     CDS             14914..15582
                     /gene="radC"
                     /locus_tag="ACHOSM_RS16940"
                     /old_locus_tag="pgaptmp_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGU4559837.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            15799..16035
                     /gene="rpmB"
                     /locus_tag="ACHOSM_RS16945"
                     /old_locus_tag="pgaptmp_003389"
     CDS             15799..16035
                     /gene="rpmB"
                     /locus_tag="ACHOSM_RS16945"
                     /old_locus_tag="pgaptmp_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGU4559838.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            16056..16223
                     /gene="rpmG"
                     /locus_tag="ACHOSM_RS16950"
                     /old_locus_tag="pgaptmp_003390"
     CDS             16056..16223
                     /gene="rpmG"
                     /locus_tag="ACHOSM_RS16950"
                     /old_locus_tag="pgaptmp_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGU4559839.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            16321..17130
                     /gene="mutM"
                     /locus_tag="ACHOSM_RS16955"
                     /old_locus_tag="pgaptmp_003391"
     CDS             16321..17130
                     /gene="mutM"
                     /locus_tag="ACHOSM_RS16955"
                     /old_locus_tag="pgaptmp_003391"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HGU4559840.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKLGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(17169..17648)
                     /gene="coaD"
                     /locus_tag="ACHOSM_RS16960"
                     /old_locus_tag="pgaptmp_003392"
     CDS             complement(17169..17648)
                     /gene="coaD"
                     /locus_tag="ACHOSM_RS16960"
                     /old_locus_tag="pgaptmp_003392"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGU4559841.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(17656..18933)
                     /gene="waaA"
                     /locus_tag="ACHOSM_RS16965"
                     /old_locus_tag="pgaptmp_003393"
     CDS             complement(17656..18933)
                     /gene="waaA"
                     /locus_tag="ACHOSM_RS16965"
                     /old_locus_tag="pgaptmp_003393"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HGU4559842.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
BASE COUNT         4392 a         4943 c         4823 g         4996 t
ORIGIN      
        1 taatgtgtat gagcgtcgtg agggcgctgg tgatggtgtt cgtcgaaagg tggcgcgtta
       61 tggcctgcct ggtcattctg ttgtggtgcg tgacggtgag ggccggaacg gtgcaattga
      121 cggcgaatgt atacgcatca atgctacaga tgtggtgctg gtggaggggc aataaaaaac
      181 cctgaaagat ttctccgggt gctccagttg cacgtggcag aaatccttca gggtcagccg
      241 taacgggtct ctgtacagag ggctccagtt gcccagagat gagcacccgc tacagcctgt
      301 tgtgataagc cgctttacag tgagctccag ttgctcaaag aggcgcgttt ttatcacaac
      361 ctgaccgcga taacccgctg tacagagggc ttcaattgcc caaagaggcg cattcttatc
      421 gcggcatgac gaaatcaccg tgatgttttc gtcgttcact catcccgatg tgtgggagat
      481 gcaccggaat gagtgccgaa aagccgctgg ctggcttcat gcagtcggac tcagttgccc
      541 ggggcgaaca ccatcaacgg ccttttggtg gtgattatag cgaatcgcga taattgagca
      601 aatacggtga tgagatgcgt atcacggtag cgctaaatcg tcgcctgcat cactgaagct
      661 gtaatttctc cattcatcct gcggattaat ccccatctct ttcagcgttc tgtctgcggc
      721 ctttttcaca tcgccaaagg tgacgaactg ctcaggcata cgacgggttt tactgatgca
      781 tatcgcccga cgtctggcag cttcctcgcg ttcactgtcc gtttctgcgt gaagaagaac
      841 ctcagcccat cgggtcgccg cacgtcgcca taaaccgcgc tcctcaagtt tctgtgccac
      901 gctgtccgtc atcactggct ttacctccgg ctgattactg ctgactgttt ttctgcttca
      961 tctgctccag tctccgcttc agtagttcgc tggctactga tttatcttct tccgtgactt
     1021 caccgcaagg ttgcccgttc agtccatacc gtgcgccgcc agcgacaatg gctttcaggt
     1081 atcgcgtcgt gtggatgtat gaggttaatg cgccacgtaa tacgccatga ccaatcgcca
     1141 gttcccgcgc cttcgcgtcc tgcaacatat cgtgtatcac gccagcctta aggggtttta
     1201 tattctctgc cccaaacagt tcaggccagt actcagccag tcggtttatc ctcctgcggt
     1261 tttttcgctg ccgctttgtc atgtgctgcc acggtatgcg ccgtgatggt ttacgtgtgg
     1321 tctggtcctg ttgtgtggtc tgctggttca tgttgtgctc ctgtcattca gaacggaata
     1381 tcgtcagaga acgggtcatc acctcccgct ggtggctgat tcccccgtgt tccggtctgt
     1441 cgttttgctc tttccagtgc ttcggtcgcc ttcccctgtt gcccccgctt cccgccagga
     1501 cgcgccgaac gggcactgat tacgctgtca gcgataacct gccagccctg ccgtatttct
     1561 ccgttctgtc cggtccactg actgatctgc atgttacccg ccacgctgat aaggtcgcct
     1621 ttttcatgtc tcgccagcgc gtcggcctgc ttaccaaatg ccaggactga tagccataac
     1681 gtcgcctgtc cgtcctgtgc ctgactgcat ggcaaagata ccgccatacg cgccagcgtc
     1741 atcggtgtgc ccttgctggt ctgttttacc tgcgggtcgt ccaccagccg tccgtaagct
     1801 gcaatctgtg ctgtcatggt ctgcgctcct tgttccggta ctcttggata atacccattg
     1861 tcatatcgaa taaatcacat gccgcttttt ctgcatcttc cgctttcagg tcatacagcg
     1921 tcatgaaatg gtaagtgatc gcccgtgctg ctcttttgct gtttgctgca tatttctcat
     1981 agatttttcc tgcccgctct tcaatatccc gtcggaactc ttcacgttca aacaggtcac
     2041 ggtgtatcaa catcagcgaa tcattcagcc atgtcacatc ggcgtatacc gttcgcttcc
     2101 agacaatcag gatcggaagt ttatgtttgt cgcagtgatg cagatgttcg tcaggtatat
     2161 aaagcgtggc tctgcctcgt accgatacga acctcacaac agattccggt tcggttaaat
     2221 cgatagcgtg tgaatgtgat tttttggcgt gtttctctgt cataaaattt ttctcaaagg
     2281 aataaataaa aatttatgcc caaaaggtgt tcaactgttc accttttgct atttcgcctt
     2341 tagattcatg aagttaaggg gtgaacgctt tttgtaaagg tgttcaccaa ctgttcaccc
     2401 tgctcacccg tgactgttca aaaatcatac aaaaaggtga acaggtgaat actttggtga
     2461 atagttttac aaaaagtgtt cacccattaa catattgata ctaaatacat ttattgcagg
     2521 gtgaacagtg gtgaacagtt tttctataac ttaattttta tcaccctcat ttttcactgg
     2581 tgcacatgac ggcagctagt cctcagactc atccgtcagt atcacgtttg atcgcacacc
     2641 ttctttagtt ctctttttca tatactccct gccatattcc gccattgcgc ctggcatatc
     2701 tgcaccgaag cgcgtcagtg ttacaggttt accaaaccca tgtgcccgca tataagccag
     2761 ataggcatga tagagatacc tgcgtgggct gaatggtaca atttcagcat tacccactaa
     2821 caggccgtca cacattaccg acgacatgag atagccgcag aagtccacca gcgaatcccc
     2881 ctcacgcttt atcgccagtg cttcttctga tttctgctgc tcatataaaa ggcgtctggc
     2941 ttcgtcctga tcagcaaacc gtgtaagcag atgacgaatc actacagcaa gctcaccttc
     3001 tattttttcc ggcagcatcg gatcgcgttc gttttccggt acaacctccg aaaaattgaa
     3061 tatcacacga cgacgtgaga tccccccgct gcggtcactg aatgacatgg cgctgttgtt
     3121 aaccgccagc acaaccgccg gaatacgcgt tgagtatggc gctttgtgtt tcgggtcaat
     3181 tgccaccttg tcaccgcccg taatggcctt tatccctgca ccatcacccg cgtagcgggt
     3241 catatccggc agaataatca gtgaaaagcc aaccactaat gcgcgttccc ttgcatcctc
     3301 cagtgccttt atgcttgccg ataccgtgtt ggccttacct gccagcatgg tacaaatctc
     3361 cgccataaca cttttaccgc ttccaccagg acctgttacc tcaatgaata actgccagtc
     3421 gtaccggttc gccagcacca taaataacgc tgccagaacg cgatctgcct tgcggtcatt
     3481 ctcagccacc gaacggcgca accatttcca gaaattcggc gcatgtgttg ccagcgtttc
     3541 cccctctgct ggtgggctga aaggtaattc actggcaatt aacaaccagt cgtttttgtt
     3601 atgctcccga aaattacctg ttctggtatc aaataccccg ttactgaatc caatcaggtt
     3661 acgggctgta ttccccatta caggcaaact taacttcatg gtatcgaccg ccgatttaat
     3721 ggcgttctgc gaatagctga tctccgcatc aatgaaaatc tgtgccatag ctcgctgtaa
     3781 ttctttatcc tgtactggct cccatacaac gccgttgtaa tggtgaacag tgtcagagtc
     3841 agcatgaatc gccagttcac cgccataatg tgccaggaga acttcgccgc gttgacttgc
     3901 tcccatctgg ttaagcgcta gtgatgaagc gttatcgtct tttacccgct cttttttctt
     3961 tacaggcagt tcaactacct ttttcttttc cgcctgctct gcccgttcac gttccagata
     4021 ttcgcgccag ttctcccgtt tctggctgtg cattccttca gggtaataat cagcatccct
     4081 gacacctgcc actgccagtt tctgcccgat ggtattaaca agccccggac gcaataaccc
     4141 cgcctgatag agacgcacgc gatagcgtcc gtccggtacg atttgcaggt tgtccagttc
     4201 ggcaagttgt tgctctccaa gccagacagg tggcacgtta tcgccagcca gtcgcccgtc
     4261 ctgttcctgc cactgcttcg catgtgccca cgcatcactg ccagcgaaaa taatcacttc
     4321 cgtatcggcg tgttttttcc ctcgaggctg tttttttaag ttcggtgcgc ttttcatttc
     4381 ttacctctgc tgatcatcgt catgtggcta ccgtattcat tgcggtaaat atccccgcac
     4441 ataaattcgg ggtgagtttt gctactggca gttaagccag tattttttga tgccattgaa
     4501 ttaatctcct gaaggagtcg tcttaatcct tatttcccgt ttatttttag ccgttattgt
     4561 tcgccgcgtt tttcttcctc cgtcattaac cacaccgcca cattgctgga aagttgtttg
     4621 ataagtcctg caataacaga aaggtcatgg tcgtctattt cttcgtggtt gcgttcaatc
     4681 atgcggctga ttacttcgac ctgttgcgct tgcagtgagg cttctgccag tgttatttgc
     4741 tgcattactg caccccctta atttcatgat attcagaaca acaaaggatt cagtcattga
     4801 cgcatattgc atcatcaccc gatgcagcaa tcagccagct acctgcctta cctaagctgc
     4861 ttaatcaggg cgagcaatga ctcaatctca ccctcagtca ttccacctgt gttacccatc
     4921 atcagagaac aaataagttc tgcctgcatt gcgctttttg ctgcttcatc aacagtcata
     4981 tcggaaaaat cacacatgaa ttactcctca tcctgaatac atgtaactgc gtggataatt
     5041 acgccggata aaccggcttt agctgatttg ttaattactg tgctgattgc agcctggacg
     5101 ttttcggcct tcacgttctg tgtgatgccg atggtatggc cgcgcttgtt aacagcacgg
     5161 gaaaagacgc ggaaggtttt aagcatgagt cacctcctga cggatacggg cgacaaaaat
     5221 cagatgtgcg tgcggcagcc gggcgcgtgc ttcgcgctca gtggcagcgg taacggtgaa
     5281 tattgcaatg ctgtctgtca ggcactgcat aaagcgccag acaaagtaag ggcgtgcgga
     5341 tacagccatg tgaaaggctc ctctgacttt gggattatac ccaccgccca agaggccaat
     5401 cttgctggcg gtggactgta cggaattggc cttactggtg ttagaggaaa ccagccttcc
     5461 cgaaggaagt tccatacagc ccaccattgt tgaggtgtgc gtgtgcgccg atacaaaaaa
     5521 aaagacgctg gcgcgtcgtg tatcgcctct aactccagcg ggaggccaat cccgatcccc
     5581 gttttataag ggatagcctc agtatatgcc gcttttttgc ttaagtggtc ggatgtgttg
     5641 cgagagaagt ttgtgcgaaa aatacctgcg ctttctttga aagcacataa taacatcacg
     5701 ttatttatgt ttatgcagtg tgtcgcaaag cgacctgaat catcacgcaa attacaacca
     5761 taaaaaatac ctgcgctgtg atatgaggta ctacagcgca ccgctttacg ggtacagaca
     5821 atgacaaatg aagaattaca ggaaatcgca ttatccatac ttgagcgaca gcgcgaaaaa
     5881 gcacaacggc aaaggaggtt gttacttaat ccactgccac tcaccaggcc aacacgattt
     5941 gcccttgaag attttgtcga gaaataccca cggcggttaa aagccactaa aaaccgaccg
     6001 cctggctgat agccttgtcc accagctaca ctcatggcat agttggtgat gcgcttgatt
     6061 tcctttatct ccattggcga cccttccgtg gtcgcctttg ttttgcctgc cattcctgca
     6121 tcatgcggat attcgcgcca tacagggcca ccagctcgat aatttaataa aacctccttc
     6181 tgataatgct taacgtgttt catgcgctca gcggcgaaat ggctcacgat atggtgcatg
     6241 taatcagaat ggttgaggct cagttgtgcg aacgtatccg ccgtgtcgat gtacagggcg
     6301 gcagcgttgc ggatgtcagg catgttcatg ctctcccatg cgacgggcca gccagcgctg
     6361 tgaaaggcgg attaattcgg cttttcgctg ctggtaagtc atacccaact caatcagcgt
     6421 gatattgctc tggtcaaggt aggaaaggtg ttcaagctgc ataacgctca tgctgtcacg
     6481 cggttttcct ttgatgccgt tcgcctgcgc ccattgtttc gccgtcatgc cgcccagcac
     6541 gatacgcgcc agcatatttg attctgtggt gtagtggtgt tgatgagtct cttttcccaa
     6601 ctctgcgcgg tacaactcca gcgcagcgca cataggctta aaaaaactgg caacggtgac
     6661 acgcgctttc agttcacggc gcaaagctgc ggcacgttcc ggcgctgcct tgtgtagctc
     6721 ttcttcacat ttgatgaagt actggcgaat tgcgcggccc tgttcagtgc gttcgaccat
     6781 cgccagttct ttagccatgt tcgtagagat caaatagtct tcactgcggc gatcaccgcc
     6841 gcgctttgtt gtccagccag aatcaaactg ttcaatattt acactttggc tcccccgttt
     6901 cggggaatca aagtacaaat tttctacaac ctcaaaatcc acaccttttt taaatccgta
     6961 ctcctcaata cgccctttga tccaggtagt gaaatcacgg cctacaccga gcgctttatg
     7021 cagtgcgcgt gcgctgacaa tgcccgtttc atatccgcca ataatgccag gtgataccgg
     7081 aataattgcg gcgaaatact gttggctcag ttcttcggac tgaacgaagc cctgcccctg
     7141 tacgggcata ttcggacgtt tcattgaaaa ctcctgctgt cgaatcaggt ttggtgctga
     7201 cgggcgcgga actgtacgcc cgtcatgctg atcactgctt ggtgttgttg cggcgctcgt
     7261 tcagccagtg gttgatctcc accgcattaa atacggtgtt tttctccccc agcttcgtgg
     7321 gcttcggtaa tttgccgata cgcacccagc ggtcgatagt tgacacgctg gtatcaagca
     7381 acttagccag gttgcagcgg cggatgtagc ccgtggtagg gatagcttct tgatggttag
     7441 ctgtcatgat tccctcttgg gttaaaagtt gatgttttct ctattctatt ggttgtttgt
     7501 acagcattcc atcaaatctg gaatctatca aaatattcac agattctatt gaaataatat
     7561 atttccagta aaagattgag tgagcgggaa gaggtgctaa gactttgaaa gtttttgctt
     7621 tattggcgat aggtcactga atccgtatac atcctgatac cgtgatatac ccaatgcgtc
     7681 gttatgcgct gttatggttt gccatctttt gccagttttt aacgtgttaa tcctgtttat
     7741 accgatttat aacgattaat aacggtgaat aaattttgct ggtgggggat gtatggggat
     7801 tacagccccg tgaatacagg gcgtgttggc tggttaatct gcctggctta ctgccagtaa
     7861 tgcggctgat attttttgca taaacgtgga tttggctagc ccttttggcg atatcccttt
     7921 ctccttcatc agcgcttcca tgtcgcggta aatggctgaa cggtttggtt ctccgtcctg
     7981 aatgtatgcg gttgtgcctg attttttcac gaggagtaaa accagcaacc ccatcatctt
     8041 catgtttccc tgttcttcgt cgagtttcct gtgctgcccc cttccgctgc ggcttgtttc
     8101 tttgatgtaa cggaccagat cccgcccgcc aagattaagg gctttttcct cccagttttt
     8161 tgtcccacgc aaatcatcga tggtatctgt taccgccttg attattgcct ctggcgtgcc
     8221 tgcctccatg aacgggaagg aagcaaggaa tatatccccc gctctgtatg gctcatcctt
     8281 gcttgttttt actccactat gcagcctgat agcacgtgcg atatgagttc tggctgattc
     8341 gacctgttcg aacttgtcct gcggtacgtc gcctatcctc attgacggat taagatcagc
     8401 taacgcaagg gccatttcgt ggggagtgat tgaaacagaa gcgcaaaggc gctctattgg
     8461 cgatttcttc aggatgttaa tcatgattta gcccttctga ttggtgtcac gttgtagtgt
     8521 tctccgcgtt ccagcgacac taacagagtg gtccacttcg ccagtgcgtc ccgtcgttca
     8581 ttgaaatact gatagcggtt gtatatgcct tcgactccct tgatgcggtg attgaggcat
     8641 ctttcagcga ctaccggatc aacaccaagc gcagctagat gtgtgcgtgc tgtacggcgg
     8701 aagtcgtgaa tagtgaaatt tggaacttcc ggcatctctg cgcggacttt tgccagcgca
     8761 acaggtaggg tgctctcctg aatgtggggg atcatcctgt gctgcatttt ccgcgccgga
     8821 agcacccagg cactattgca tgacaacaca cgcaactctc gcagccattc aataacggga
     8881 tggacgagtg gaatatcgat ggggtcgccg tttttgctgc gttcctccgg cagatgccat
     8941 acagcgttat caaggtcgaa ttcttcccat ctggcggcgc acaactccat cttcctgaca
     9001 caaagcgcca gcaatagctt aaatgtgagt tcattctcgc ggctgaaacc ctttgcggtc
     9061 cgcatagcct gaaagaatcg gattagctct tcacgagtca gccaacggtc gcgggaggac
     9121 tccttgccac cagcatcaga gacttcaaac gcagagcatg ggttaatctc cagcacatga
     9181 cgttttaccc cgtagtcgaa tatgcggcgc gtccagcgta gaacgtccgt tgcaatggtt
     9241 ggcgctccac ggtcaacaat tgcctttagc atctcgtcaa tgtgtcgtgg ttttacgtcc
     9301 tcagccttca tatgaccaat acgcgggttt atgtctttgt cgatgcgacg gcgcaggatg
     9361 tcgggatgtt tccatcgtgg cagtatctga cgttcgaaat attcggcagc aagctctgat
     9421 acgcgcacgg cctttttttc tgcctcaatt tttgccaggg cttcggcctt acgctcctgt
     9481 ttctctgctg ctacgtcgta cccaagtgcg acgcgagcgg atagttcctt tgctgtttct
     9541 ctggcttttg atagcgagta atcagcatag gaaccaatga ccattgcccg tgcttttcct
     9601 gcgaatttgt agcggaatcg ccatgttggt atctggtctt ctttccggta acgaaggtat
     9661 aagccaccgc catctgaccg cccttcgaat cgttcacctg attttatcca tgccctgatc
     9721 tgaatatctg tgagctttgg cattaagaac tcctgaacat tgaatagaga ggtgtaccca
     9781 aacagatgtt ccaaaatgca attgggtaca cctttgggta cacgctaatc atgcgctttc
     9841 ttgcgtcgtt atgatagcca ttaattacaa aaaaacccgc attatcgcgg gtttgattga
     9901 attattgcgc tttattgatt ccctctgata gcttactcca cattttgaat ctgctcgcgc
     9961 atctgctcaa tcaacacttt cagctcgatg gcggagtttg tcacttcggc attgatagat
    10021 ttcgacgcaa gagtgttcga ctcgcggttg aattcctgca tcataaagtc cagacgacga
    10081 ccaacggctt cttttttctt cagaatgttg taggtctctt tgacatgcgc ttccaggcgg
    10141 tccagttctt cggcaacgtc aattcgttgt gccagcagaa ccaactcctg ctccagacgg
    10201 ttgttttcca actgaacctg agcatcttcc agcttcgcga ccagacgctc acgctgccat
    10261 tgcaggattt ccggcatatg ggcgcggact ttgaccactt cggcggtgac gccttccaga
    10321 cgctgctcga tcaatgcttt cagtgcctga ccttcggttt cgcgcgcgac aataaagtcg
    10381 tccagcgtac catccagcgc cgcgagaatt tcagcggcaa tggcgtcgag atcctgctcc
    10441 tgggctgcca tcacgcccgg ccagcgcaga atatcaaccg ggttgatttc cccttcgtca
    10501 ctctgcattt ttacccagtt cgcggcagtt accagctgtt tagccagttt ttcattgagg
    10561 attagctcac cttgtgcgct aacatctggc tcatagcgca gggtacattc cactttaccg
    10621 cgcgtcaggc gagaacgaat acgctcgcga acgacaggtt caaggctacg gaactgctcc
    10681 ggcagacgaa agtaagtttc cagataacgc tggtttaccg agcgcatttc ccaggttgcg
    10741 ctcccccatt cacccttgat ttcacgccgg gcgtaggcgg tcatactgcg gatcatagac
    10801 gttcctgttt ataaaaggag aggtggaagg attatagcca tcgatgcctt gtcaggatag
    10861 gaataaccgc cggaagtccg tataatgcgc agccacattt gtttcaagcc ggagatttca
    10921 atatgcgtcc agcaggccgt agcaataatc aggtgcgtcc cgttaccctg actcgtaact
    10981 atacaaaaca tgcagaaggc tcggtgctgg tcgaatttgg cgataccaaa gtgttgtgta
    11041 ccgcctctat tgaagaaggc gtgccgcgct tcctgaaagg tcagggccag ggctggatca
    11101 ccgcagagta cggcatgctg ccacgttcta cccacacccg taacgctcgt gaagcggcga
    11161 aaggtaagca gggtggacgc acaatggaaa tccagcgtct gatcgcccgt gctcttcgcg
    11221 cggcagtaga tttgaaagcg ctgggtgagt tcaccattac gctggactgc gacgtgcttc
    11281 aggctgatgg tggcacgcgt accgcgtcga ttacgggtgc ctgcgtggcg ctggcagatg
    11341 cgctacagaa gctggtggaa aacggcaagc tgaaaaccaa cccgatgaaa gggatggtag
    11401 ccgcagtttc tgtcggtatt gtaaacggtg aagcaatttg cgatctggaa tacgttgaag
    11461 actcagccgc agagaccgac atgaacgtag tgatgaccga agacgggcgc atcattgaag
    11521 tgcaggggac ggcagaaggc gaaccgttca cccacgaaga attactcacc ttgttggctc
    11581 tggcccgagg gggaatcgaa tccattgtag cgacgcagaa ggcggcgctg gcaaactgat
    11641 ttttaaggcg actgatgagt cgcctttttt ttgtctgtag aaaagtaaga tgaggagcga
    11701 aggcatgaaa ccatatcagc gccagtttat tgaatttgcg cttagcaagc aggtgttaaa
    11761 gtttggcgag tttacgctga aatccgggcg taaaagcccc tatttcttca acgccgggct
    11821 gtttaatacc gggcgcgacc tggcactgtt aggccgtttt tacgctgaag cattggtgga
    11881 ttccggcatt gagttcgatc tgctgtttgg ccctgcttac aaagggatcc cgattgccac
    11941 cacaaccgcc gtggcgctgg cggagcatca tgacctggac ctgccgtact gctttaaccg
    12001 caaagaggca aaaaaccacg gtgaaggcgg caatctggtt ggtagcgcgt tacaaggacg
    12061 cgtaatgctg gtagatgatg tgatcaccgc cggaacggcg attcgtgagt cgatggaaat
    12121 tattcaggcc aatggcgcga cgcttgctgg cgtgttgatt tcgctcgatc gtcaggaacg
    12181 ggggcgcagc gagatttcgg cgattcagga agttgagcgt gattacaact gcaaagtgat
    12241 ctctatcatc accctgaaag acctgattgc ttacctggaa gagaagccgg aaatggcgga
    12301 acatctggcg gcggttaagg cctatcgcga agagtttggc gtttaaagaa actcgccgga
    12361 tgaaaagtca tccggcgtca tattactgca actgcgcggc aattagcggc cagcgggcgt
    12421 caaaatcatc cgtcgggcgg tatttaaatt cgctgcggac aaaacgtgac agcatacctt
    12481 cacagaaggc caggatctgg cttgccagca gggtttcatc ggtggtgtaa ccttcaccct
    12541 cacgcattct cttttcacgc aatacctggc gcagctgcgc ttcaatacgc tcgaacagct
    12601 ggttgatgcg cccttgcagg cgatcctgtt caaacattag cgcatgacca gtgaggatgc
    12661 gggtcaggcc aggattacgc tcaccaaaac cgagaagcag caacacaatc agacgcaggc
    12721 gcgctgtggt gtctttctca tctttcagaa tcaggttgat gcgagtaatc aggctatctt
    12781 cgataaactc aatcagacta tcgaacatgc gggtcttact ggggaagtgg cgatacagtg
    12841 ccgcttcgga aacgccgaca gaggcggcca gttttgccgt cgtgatacgt tggcttccat
    12901 cgctggattc cagcatcagc gccagagact gaagtatttc ctcgcgacgg ttccttttcg
    12961 cagtttgttt ttctgccatg ttacaaaata cccctgaaaa taagcacttg ccaggcggca
    13021 cccacgctat gaccgcaaac gaaatgtttg cggctatgtt atgacgttat tcggatgcgt
    13081 atgtgttact gacgaccaga gtgaccaaag ccgccttcac cgcggtcggt ggcgtcgaaa
    13141 tcttccacca gattaaattc agcctgtact accggaacaa aaatcatctg ggcgatgcgt
    13201 tcgccaggtt gaatggtgaa gctgtcctga ccacggttcc acacggaaat catcaactgg
    13261 ccctgatagt cagaatcgat caatcctacc aggttaccaa gcacgatacc gtgcttatgt
    13321 cccaatccgg agcgcggcag catcattgcc gccagtgaag gagcggcaat atgaatcgcc
    13381 agcccggtcg gaaccagcgt agtgtcaccc ggagccagtt ctacggcgtc gtcgagacag
    13441 gcacgcaggt caagtccggc agagccagag gtggcataag tcgggagcgg aaattccttc
    13501 ccaacgcgcg ggtccagaat cttaacgtcg atttttttca tcataacggg tcacgatctc
    13561 gtcgagtaat aattggccaa ggagctcttt gcgctcaagt ggtaagactt tatctccgtc
    13621 ctgccagaaa aggtgtaatg cgttgttgtc gctgttaaat ccttgagttg gctgggaaac
    13681 atcgttcgcg cagatcagat caaggttttt acggatacgt ttttgccggg cgtattcttc
    13741 cacattattt gtttcggcgg caaatccaac gacgtagggt cgatggtctt ttagtgcggc
    13801 aacgcctgcg acgatatcgg ggtttttaac catttttatt gttaattcat caccctgcgt
    13861 ggcctgcttt ttgattttct ctggggccac ggtagctgcg cgataatccg ccacggcggc
    13921 gcagccgata aaaatatttt gctgctgtac agaagcattc acggcggctt ccatttccag
    13981 cgcggtcatc acatcaacac gtttaacaaa cggtggcgtc ggtagtgaaa ccggacctga
    14041 taccagcgtg acgttcgcgc cacgacgggc agcggcggcg gcgatagcaa aacccatctt
    14101 gccggagctg tgattagaga tataacgcac gggatcgagc ggttcacgcg tcgggccggc
    14161 ggtaatcata atgttcagat gtttcaggtc gttgacgggc gaaaaatgcg ctaccgccat
    14221 atccacaatg gttaacggat cgagcattcg cccaggaccg atatcaccac aagcctgact
    14281 gccactgtct ggcccccaga tgagcacacc acgggaagca agcacctcta aattatgctg
    14341 cgtggcagcg gcacggtaca tctgctggtt catggcgggg agcacggcta caggcgcagg
    14401 tgtagccaga caaatcgtcg ataccaggtc attcgccatt ccggcagcaa cacgggcaat
    14461 caaatctgcc gtggcagggg cgagaatcac taaatcagcc catttaccca gctcaatatg
    14521 gcccatagcg gcttcggctg ccgggtccag cagactgtcg gaaacgggat aaccagaaac
    14581 cgcctgcaag ctaagtgggg tgataaaggc ttttgccgct tcggtcatgg ctacgcggac
    14641 gtcggccccg cgatcgcgca aacgacgcac cagttcaggg gttttatagg cagcaatacc
    14701 gccgctaacg ccgagaacga tttttttacc ggccaggctc atgatgattt ttcctgtagg
    14761 gtgacaccag aagttggcga ttttatcaca atacttttgt tgtcgtgcct tcacctgaga
    14821 ttcactttgc gaggcgcttt ccaggattga aaactggccg tcgatttaac gaaacggcta
    14881 tgacaggatg cgagcaccac aaaggaggtg aaggtgaaaa acaatgcaca gctgttgatg
    14941 ccgcgcgaaa aaatgctgaa gtttggtatt agcgccttaa cggatgtcga gctgctggcg
    15001 ctatttctgc gtaccggaac gcgcggtaaa gatgtattaa ccctggcaaa agagatgctg
    15061 gagaatttcg gctctcttta tggcttgtta acctctgaat atgaacaatt tagtggcgtt
    15121 catggaattg gcgtagcgaa atttgcccag ttaaagggga ttgctgaact ggcgcggcgt
    15181 tactacaacg tgcggatgcg tgaagaaagc cctttactca gcccggagat gacgcgggaa
    15241 tttttacaaa gccagctcac gggtgaggag cgggagatct ttatggtgat ctttctcgac
    15301 tcacaacacc gggttataac gcatagccgt cttttttccg gcacgctaaa ccatgttgaa
    15361 gtccatcctc gggaaattat ccgggaagcg ataaaaataa acgcctcggc gctgatcctt
    15421 gcacataatc acccttcggg ttgtgctgaa cccagtaaag cggataaact cattactgaa
    15481 cggataataa agagttgtca gttcatggat ttacgcgtgc tcgaccatat cgtgattggg
    15541 cgtggagagt atgtttcttt tgccgaacgc ggctggattt aacccgctat gcgcgatcct
    15601 tcgggatctt tgtctgttcg ggacttgagc acatcgctga gtcagcgtat actacgccac
    15661 ctttgagaat ctcgggtttg gcatttgggc ctggcaatcg agagttcaca tagaactgcg
    15721 atgaccgggc tgtaaagcct gacgaggcgc caatacccca tacgaagctc gagctaattt
    15781 gatttttgga gaatagacat gtcccgagtc tgccaagtta ctggcaagcg tccggtgacc
    15841 ggtaacaacc gttcccacgc actgaacgcg actaaacgcc gtttcctgcc gaacctgcac
    15901 tctcaccgtt tctgggttga gagcgagaag cgttttgtca ccctgcgcgt atctgctaaa
    15961 ggtatgcgtg taatcgataa aaaaggcatc gatacagttc tggctgaact gcgtgcccgt
    16021 ggcgaaaagt actaagtact tagaggaaat aaatcatggc taaaggtatt cgtgagaaaa
    16081 tcaagctggt ttcttctgct ggtactggtc acttctatac cactacgaag aacaaacgta
    16141 ctaagccgga aaaactggaa ctgaaaaaat tcgatccagt tgttcgccag cacgtgatct
    16201 acaaagaagc gaaaatcaaa taattctcgc tttgatgtaa caaaaaacct cgctccggcg
    16261 gggttttttg ttatctgctt gcccccatat tgattgcatc tgttcattcc tggagatgct
    16321 atgcctgaat tacccgaagt tgaaaccagc cgtcgcggca tagaaccgca tctcgttggt
    16381 gcaaccattc ttcatgcggt ggtgcgcaac ggacgcttgc gctggccggt ttcagaagag
    16441 atctaccgtt taagcgacca accagtgctt agcgtgcagc ggcgggctaa atatctgctg
    16501 ctggagctgc ctgagggctg gattatcatt catttgggga tgtctggcag cctgcgcatc
    16561 cttccagaag aacttccccc tgaaaagcat gaccatgtgg atttggtgat gagcaacggc
    16621 aaagtgctgc gctacaccga tccgcgccgc tttggtgcct ggctgtggac caaagagctg
    16681 gaagggcaca acgtgctggc tcatcttgga ccggagccgc ttagcgacga tttcaatggt
    16741 gagtatctgc atcagaagtg cgcgaagaaa aaaacggcga ttaaaccgtg gctgatggat
    16801 aacaagctgg tggtaggggt agggaatatc tatgccagcg aatcactgtt tgcggcgggg
    16861 atccatccgg atcggctggc gtcatcactg tcgctggcag agtgtgaatt gttagctcgg
    16921 gtgattaaag cggtgttgct gcgttcgatt gagcagggtg gtacaacgct gaaagatttt
    16981 ctgcaaagtg atggtaaact gggctatttc gctcaggaat tgcaggttta cgggcgaaaa
    17041 ggtgagccgt gtcgggtgtg cggtacgccg attgtggcga ctaaacatgc gcagcgggca
    17101 acgttttatt gtcggcagtg ccagaagtaa ttcatgcgcg ccggatggca taccatccgg
    17161 cataaacgct acgctaactt cgccatcagc gcctgatgga cattctccgg caggaaatgg
    17221 gtgacatcgc cctgatggcg tgccacctct ttcaccaacg atgaagagat aaacgaccac
    17281 tctttcgacg gcatcagaaa cacactttcc agttccggca ttaagtggcg attcatatgc
    17341 gccagctgca tttcatattc aaaatccgcc accgcacgca ggccacgaat cagcaccgta
    17401 gcgtgttgat tacgggcgaa gttcgccatc aaatcactaa acccgaccac ttccacgttc
    17461 cccagatgcg cggttgcctg ctgtgccagt gccacacgct cttccagggt aaacatcggt
    17521 tttttactgg ggctggcggc aatcgccaga ataacgtgat cgaacatctg cgtggcgcgc
    17581 gtcacgatat cgatatgacc attggtaatg ggatcgaaag tacccggata aatcgcccgt
    17641 ttttgcataa caacctcaat gcgttttcgg tggcaggtaa ggttccagca gttgaagcag
    17701 acgctgtagc gcgccctggt tttgatacag tacttcaacg gcatgacggc catagaaact
    17761 acggtaatcg gcgtcggtga gtaaagagga aacctctttt gcaagcgtag tggcatcggt
    17821 aacggtaata agcccgcttg cctgctccag ccgcgcgcaa atgtctttaa agttaaaagt
    17881 atgcggcccc atcaataccg gaatagcgtg tgcggcagct tccagcggat tatgcccacc
    17941 acgttcaacc agtgaaccgc caacaaaggc gagatcggca atgccgtaca gtaacatcaa
    18001 ctcgcccatc gtatcgccaa ccacaacctg cgtgctggtg gaggggactt cccctgaaga
    18061 gcgtgtgata tagcttagtc cagcctggcg gacaaggtta atcgcatccg ggaagcgttc
    18121 tggatgacgg ggtaccagga tgagcaataa attcgggaat tgctgtaaca atgcctgatg
    18181 tgcggcaatg accacgctct cttcaccttc gtgagtgctg gtggcaatcc ataccgggcg
    18241 gtgtggtgcc cattggcggc gtagcgtcac ggctttagca gccaactgcg gcgttacaga
    18301 aatatcgaat ttcaggctac cggtaacggt tacctggtta ttttttgcgc ccagcgccac
    18361 aaaacgtgca ccatcttctt cattttgcgc agcaatcagc gtaatacgac gcagcaagcg
    18421 gcggacgaat ttacccagtt tggcataacc tgcggccgag cgggcagaga gtcgcgcgtt
    18481 agcgatcacc agcggaattt tacgtttatg tagcgccgca atcaggttag gccatagttc
    18541 ggtttccata atcaacacca gtttggggtc gactttattc aggaaacggt tgagcgcatc
    18601 gggcaggtca tacggcagat aaacgtgctg aacatccttc ccgaaagccg attgtacgcg
    18661 ctccgaaccg gttggcgtca tggttgtaac ggtaatcggt aaatcaggat aacgatgacg
    18721 cagcgcgcgc accaacggga tcgccgccag agtttcaccg acggagacgg agtgcagcat
    18781 aatgccgcct ggttttagcg gatggcggta aaaaccgtaa cgttcacccc agcgttttcg
    18841 ataggccgga gccttacgtc cgcgcaccca gagccgtatc cagatcagcg gctgaataag
    18901 gtagagaagg gcggtgtaaa gcaattcgag catagtaaat agctgactta tggatgtgct
    18961 ggggattcta tgtatttagc tgtggcttta ccattacttt tcccgttttt gacttaaata
    19021 gcaatttacc cgccaattct tgctgaacat ctatactttt tttgtatttt tatgcgattc
    19081 attgatttct gctgtacgtg caggaaggaa tcgcagagca acagtgaaag aatatggttg
    19141 ctaatgattt tatt
//
LOCUS       DAZKHC020000079        18748 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZKHC020000079 DAZKHC020000000
VERSION     DAZKHC020000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.079"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            149..877
                     /locus_tag="ACHOSM_RS16970"
                     /old_locus_tag="pgaptmp_003394"
     CDS             149..877
                     /locus_tag="ACHOSM_RS16970"
                     /old_locus_tag="pgaptmp_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU4559843.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNAIWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKKINAVSTLITRNSEHH"
     gene            877..5727
                     /gene="ehaG"
                     /locus_tag="ACHOSM_RS16975"
                     /old_locus_tag="pgaptmp_003395"
     CDS             877..5727
                     /gene="ehaG"
                     /locus_tag="ACHOSM_RS16975"
                     /old_locus_tag="pgaptmp_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HGU4559844.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGHSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNISGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVTLGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNATDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6095..7750
                     /gene="lldP"
                     /locus_tag="ACHOSM_RS16980"
                     /old_locus_tag="pgaptmp_003396"
     CDS             6095..7750
                     /gene="lldP"
                     /locus_tag="ACHOSM_RS16980"
                     /old_locus_tag="pgaptmp_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HGU4559845.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7750..8526
                     /gene="lldR"
                     /locus_tag="ACHOSM_RS16985"
                     /old_locus_tag="pgaptmp_003397"
     CDS             7750..8526
                     /gene="lldR"
                     /locus_tag="ACHOSM_RS16985"
                     /old_locus_tag="pgaptmp_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HGU4559846.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8523..9713
                     /gene="lldD"
                     /locus_tag="ACHOSM_RS16990"
                     /old_locus_tag="pgaptmp_003398"
     CDS             8523..9713
                     /gene="lldD"
                     /locus_tag="ACHOSM_RS16990"
                     /old_locus_tag="pgaptmp_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HGU4559847.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9899..10372
                     /gene="trmL"
                     /locus_tag="ACHOSM_RS16995"
                     /old_locus_tag="pgaptmp_003399"
     CDS             9899..10372
                     /gene="trmL"
                     /locus_tag="ACHOSM_RS16995"
                     /old_locus_tag="pgaptmp_003399"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HGU4559848.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10425..11246)
                     /gene="cysE"
                     /locus_tag="ACHOSM_RS17000"
                     /old_locus_tag="pgaptmp_003400"
     CDS             complement(10425..11246)
                     /gene="cysE"
                     /locus_tag="ACHOSM_RS17000"
                     /old_locus_tag="pgaptmp_003400"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGU4559849.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11326..12345)
                     /gene="gpsA"
                     /locus_tag="ACHOSM_RS17005"
                     /old_locus_tag="pgaptmp_003401"
     CDS             complement(11326..12345)
                     /gene="gpsA"
                     /locus_tag="ACHOSM_RS17005"
                     /old_locus_tag="pgaptmp_003401"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGU4559850.1"
                     /translation="MNQRNALMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12345..12812)
                     /gene="secB"
                     /locus_tag="ACHOSM_RS17010"
                     /old_locus_tag="pgaptmp_003402"
     CDS             complement(12345..12812)
                     /gene="secB"
                     /locus_tag="ACHOSM_RS17010"
                     /old_locus_tag="pgaptmp_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGU4559851.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGECTEEHQDA"
     gene            complement(12875..13126)
                     /gene="grxC"
                     /locus_tag="ACHOSM_RS17015"
                     /old_locus_tag="pgaptmp_003403"
     CDS             complement(12875..13126)
                     /gene="grxC"
                     /locus_tag="ACHOSM_RS17015"
                     /old_locus_tag="pgaptmp_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HGU4559852.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13268..13699)
                     /locus_tag="ACHOSM_RS17020"
                     /old_locus_tag="pgaptmp_003404"
     CDS             complement(13268..13699)
                     /locus_tag="ACHOSM_RS17020"
                     /old_locus_tag="pgaptmp_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGU4559853.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13944..15488
                     /gene="gpmM"
                     /locus_tag="ACHOSM_RS17025"
                     /old_locus_tag="pgaptmp_003405"
                     /gene_synonym="pgmI"
     CDS             13944..15488
                     /gene="gpmM"
                     /locus_tag="ACHOSM_RS17025"
                     /old_locus_tag="pgaptmp_003405"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HGU4559854.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15522..16781
                     /gene="envC"
                     /locus_tag="ACHOSM_RS17030"
                     /old_locus_tag="pgaptmp_003406"
     CDS             15522..16781
                     /gene="envC"
                     /locus_tag="ACHOSM_RS17030"
                     /old_locus_tag="pgaptmp_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HGU4559855.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16785..17744
                     /locus_tag="ACHOSM_RS17035"
                     /old_locus_tag="pgaptmp_003407"
     CDS             16785..17744
                     /locus_tag="ACHOSM_RS17035"
                     /old_locus_tag="pgaptmp_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HGU4559856.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17731..>18748)
                     /gene="waaH"
                     /locus_tag="ACHOSM_RS17040"
                     /old_locus_tag="pgaptmp_003408"
     CDS             complement(17731..>18748)
                     /gene="waaH"
                     /locus_tag="ACHOSM_RS17040"
                     /old_locus_tag="pgaptmp_003408"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HGU4559857.1"
                     /translation="KLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDGSTDNSV
                     EIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTMYETLMT
                     MALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSRRWTHVV
                     WMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLHNTSVSR
                     LHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCHAVRKEP
                     DILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHHRITRSA
                     FNLR"
BASE COUNT         4723 a         4830 c         4867 g         4328 t
ORIGIN      
        1 gaatgataca tttttatgat gaatgcttaa aacaatattt aatcgacaca catcactgtc
       61 attaatataa agcaacatag atcaccaata atacaatgga caactctacc ggcattgatc
      121 gctggtcgga ggaaataagg atttttacat gaacttgaag aagatttttt tcagtgctgt
      181 cactgtttca gttctctgcg cattaacggg atgtgactac attgaagaag gcaagccaga
      241 aagtagttta ttgaaacaac aagaagaaca taacaataaa attgatctac ttgaaaaaca
      301 acaagcacag ctaaagagtc aactggaaac tattcaaaaa caacaaactg gcataataag
      361 cagtacaaag actttaactc acgtaattaa atccgttaaa gatcaacaaa acacttttat
      421 ttttaccgag tttaacccag caaaaacgaa gtatttcatc ctgaacaatg gttcggtggc
      481 tttagcgggt cgagtgttat ccattgacgc cacagagaat ggcagtgtta ttcatatttc
      541 actggtcaac ttattaagta cacctatctc aaatattggt tttaatgcga tatggggtgg
      601 tgaaaaacct gtcgatgcca aagagtttgc ccggtggcaa caattacttt tcaacacatc
      661 aatgaaatcc acattgaaat tattaccagg tcaatggcaa gacattaatt tgaccctgaa
      721 gggtgtatcg cccaataacc tgggatatct gaagttagcc atcaacatgg aaaatattca
      781 gttcgacaat cttccctctg ctgaaaatcg gcagaaaaga agcaaaaaaa taaatgcagt
      841 tagtacgctt ataacaagga atagcgagca tcattaatga acaaaatatt taaagttatc
      901 tggaaccctg cgacagggaa ttatactgtt accagcgaaa cggcaaaaag ccgtggcaag
      961 aaatctgggc acagtaagct gttaatttct gcgctggttg cgggtggaat gttgtcgtcg
     1021 tttggggcat tggcgaatgc cgggaatgac aacggtcagg gtgttgatta cggtagtgga
     1081 tcagctggcg acggctgggt tgctataggc aaaggggcga aagcaaatac ttttatgaac
     1141 atcagtggtt ccagtactgc tgtgggttat gacgctatag ctgaaggcca atatagctct
     1201 gccatcgggt caaaaaccca tgcgattggc ggtgcatcaa tggcctttgg ggttagtgca
     1261 atatcagaag gcgatagaag tatagcactg ggtgcctctt cgtattcatt gggccaatac
     1321 tcaatggccc tcggccgtta ttcaaaagca ttgggtaaat tgtctattgc tatgggggac
     1381 tcttccaaag cggaaggagc aaacgccatt gccctgggaa atgccactaa agctactgag
     1441 attatgagta ttgctcttgg cgacaccgcc aatgcgtcaa aagcgtattc aatggcgctg
     1501 ggagcaagta gcgtcgcatc tgaagaaaac gcaattgccc tggggcgtag cagtgtagct
     1561 agcggtactg acagcctcgc atttggcaga caatcacttg ccagcgcagc gaacgctatt
     1621 gcgataggtg ctgagaccga agccgctgaa aatgcaactg ctattggcaa taatgcgaag
     1681 gcaaaaggga ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa agtcaatact
     1741 atcgcattag gaaatggcag ccaggctctg gcagataatg caatcgccat aggccagggc
     1801 aacaaagctg atggcgtgga tgccatcgct ctgggtaatg gtagccagtc gagaggctta
     1861 aacaccattg ccttaggcac agccagtaat gcaactggtg ataagagtct tgcgcttggt
     1921 agtaatagca gtgccaacgg tattaactct gtcgcgctgg gcgcagattc cattgcggat
     1981 ttagacaata ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt taatgtgaaa
     2041 aatggcgcga tcaagtctga cagttacgat gccattaatg gttcacagct ttatgccatt
     2101 agcgactcgg tagcaaaaag gcttggagga ggggctgcag tagatgttga tgacggtact
     2161 gttacagcac caacctacaa tttaaaaaat ggtagcaaaa ataacgtagg ggctgcgctc
     2221 gctgtacttg atgaaaacac cctgcaatgg gaccaaacca aaggcaaata cagcgctgct
     2281 catggtacta gtagcccaac tgccagcgta atcaccgatg ttgcggatgg cacgatttca
     2341 gcctccagta aggatgcggt taacggttcc caactgaaag ctaccaatga cgatgtcgaa
     2401 gccaacaccg ccaatatcgc tactaatacc agcaacattg ccacgaatac agcaagtatt
     2461 gccaccaata ccaccaatat caccaacctg acggattccg ttggtgacct tcaggctgat
     2521 gccctgctct ggaacgaaac taaaaaggca ttcagtgcag ctcacggcca ggataccacc
     2581 agcaaaatca ccaacgttaa agatgccgac ctgacggctg acagcactga tgctgttaac
     2641 ggctctcagc tgaaaaccac caacgatgct gtggcgacga ataccaccaa tatcgccaat
     2701 aacacttcca atattgccac taacaccacc aacatctcta atctgactga gacggtgact
     2761 aatcttggtg aggatgcgct gaaatgggat aaggacaatg gtgtattcac ggcagctcat
     2821 ggcaccgaga ccaccagcaa aatcaccaac gttaaagatg gcgacctgac gactggcagc
     2881 accgatgccg ttaacggctc tcagctgaaa accaccaacg atgccgtggc gacgaatacc
     2941 accaatatcg ccactaacac caccaacatc tctaatctga ctgagacggt gactaatctt
     3001 ggtgaggatg cgctgaaatg ggataaggac aatggtgtct tcactgcagc tcatggcaac
     3061 aataccgcca gcaaaatcac caatatcctg gacggcacag tcactgcaac cagttccgat
     3121 gccattaacg gtagccagct ttatgactta agcagcaata tcgccaccta cttcggcggc
     3181 aatgcttctg tgaatactga cggtgtgttt accggtccaa cctacaaaat cggtgaaaca
     3241 aattattata acgtcggcga tgcactggct gcgattaact cctcatttag cacgtctctc
     3301 ggcgatgctc tgctttggga tgccaccgca ggtaaattca gtgccaaaca cggtactaat
     3361 ggtgacgcaa gcgtgatcac tgatgtcgca gatggtgaaa tttcagactc cagttctgac
     3421 gcagtaaacg gctcacaact ccacggcgtg agcagttatg ttgttgatgc gctggggggt
     3481 ggtgccgaag tcaatgcaga cggcaccatc actgcgccga cgtacaccat tgctaatgct
     3541 gattacgata atgtcggtga tgccctgaat gctatcgata ccactcttga cgacgctctg
     3601 ctctgggatg cggacgccgg tgaaaatggt gcatttagcg ccgctcacgg aaaagataaa
     3661 actgccagtg taatcactaa cgtcgctaac ggtgcaatct ctgctgccag cagcgacgcg
     3721 attaacggct cacaactcta taccaccaat aagtacatcg ctgatgcgct gggtggtgac
     3781 gcagaagtca acgctgacgg caccatcacc gcaccgactt acaccattgc gaacgccgag
     3841 tacaacaacg tcggtgacgc cctggatgcg cttgatgata acgccctgct gtgggatgag
     3901 actgccaatg gcggtgctgg agcctacaat gccagccatg acggtaaagc cagcatcatc
     3961 actaatgtcg ctaatggcag tattagtgag gacagtaccg atgcagtgaa cggttctcag
     4021 ttgaatgcga cgaatatgat gattgagcag aacacccaaa ttatcaatca gctcgctggt
     4081 aacaccgacg caacctatat ccaagaaaac ggtgcgggta ttaactatgt gcgtactaac
     4141 gacgacggct tagcgttcaa cgacgccagc gcacagggtg ttggcgctac agctataggt
     4201 tataactctg tcgccaaagg cgatagcagc gtagctattg gtcagggcag ctacagcgac
     4261 gttgatacgg gtatcgccct gggtagcagc tctgtttcca gccgagtgat tgccaaaggc
     4321 tcccgtgaca ccagcataac ggaaaatggc gttgttattg gttacgacac cacggatggc
     4381 gaactgctcg gtgcattgtc tatcggtgat gacggtaaat atcgtcaaat catcaacgta
     4441 gccgatggtt ccgaagccca tgacgccgtt acggttcgtc aattgcagaa tgcgattggt
     4501 gcggtcgcaa ccacgccgac taaatacttc cacgctaatt caacggaaga agattcactg
     4561 gcagtgggaa ctgactcgct ggcaatgggt gcgaaaacca tcgtgaatgg cgataaaggt
     4621 attggtatcg gttatggtgc ctacgtggac gcgaatgcac ttaacggcat tgccattggt
     4681 agcaatgcgc aagtcattca tgtcaacagt attgcgatag gtaatggttc tacgaccact
     4741 cgtggcgctc aaaccaatta taccgcctac aacatggacg caccgcagaa ctctgtcggt
     4801 gaattctcag tcggtagtgc ggatggtcaa cgtcagatca ctaacgtcgc agcaggttcg
     4861 gctgataccg atgcggtcaa cgtgggtcag ttgaaagtaa cggatgcgca ggtttcccag
     4921 aatacccaga gcattactaa cctggataat cgggtaacga atcttgattc acgcgtcacc
     4981 aatatcgaaa acggtattgg cgatatcgtc accaccggta gcaccaagta cttcaagacc
     5041 aataccgatg gtgtagatgc cagcgcgcag ggtaaagata gcgtcgcgat tggttccggc
     5101 tccattgctg ccgctgacaa cagcgtcact ctgggtacag ggtctgtggc aaccgaagaa
     5161 aatacgatct ctgtaggttc ctctactaat caacgtcgta tcaccaacgt agctgcaggt
     5221 aaaaatgcta ccgatgctgt taacgtggca cagttgaagt cttccgaagc tggcggtgta
     5281 cgttacgaca ccaaagctga tggttctatc gactatagca atatcaccct cggtggtggc
     5341 aacggcagta cgactcgtat cagcaacgtc tccgctggcg tcaacaacaa cgacgcggtg
     5401 aactacgcgc agttgaagca aagcgtgcag gaaacgaagc aatacaccga tcagcggatg
     5461 gttgagatgg ataacaaact gtctaaaact gaaagcaagt tgagcggtgg tatcgcttct
     5521 gcaatggcaa tgaccggtct gccgcaggct tatacaccgg gtgccagcat ggcttctatt
     5581 ggtggcggta cttacaacgg tgaatcggca gttgctttag gtgtatcgat ggtgagcgcc
     5641 aatggtcgtt gggtctacaa attacaaggt agtaccaata gccagggtga atactccgcc
     5701 gcactcggtg ccggtattca gtggtaatga tccattaagt tagtgtgact aagggcaaac
     5761 gccaaattgc ctgatgcgtt acgcgcatca ggcaagataa ggcgctccgg gtcaacaatc
     5821 taagggcaat tctctgatga ggattgccct tttctttacc agacatctcc ccccacaaga
     5881 attggcccta ccaattcttc gcttatctga cctctggttc acaattcccc aattaaaact
     5941 cacatcaata ttgccaatgc ataacattta gttaaccatt cattgtcatt atccctacac
     6001 aacacaattg gcagtgccac ttttacacaa cgtgtgacaa ggagatgagc aacagactca
     6061 ttacacgatg tgcgtggact ccaggagacc tgcaatgaat ctctggcaac aaaactacga
     6121 tcccgctggg aatatctggc tttccagtct gatagcatcg cttcccatcc tgtttttctt
     6181 ctttgcgctg attaagctca aactgaaagg atacgtcgcc gcctcatgga cggtagcaat
     6241 cgcccttgcc gtggctttgc tgttctataa aatgccggtc gctaacgcgc tggcctcggt
     6301 ggtttatggc ttcttctatg gtttgtggcc catcgcgtgg atcattattg cagcagtgtt
     6361 cgtctataag atctcggtga aaaccgggca gtttgacatc attcgctcgt ctattctttc
     6421 gataacccct gaccagcgtc tgcaaatgct gatcgtcggt ttctgtttcg gcgctttcct
     6481 tgaaggagcc gcaggctttg gcgcaccggt agcaattacc gccgcattgc tggtcggcct
     6541 gggttttaaa ccgctgtacg ccgccggact gtgcctgatt gttaacaccg cgccagtggc
     6601 atttggcgcg atgggcattc caatcctggt tgccggacaa gtaacaggca tcgacagctt
     6661 tgagattggc cagatggtgg ggcggcagct accgtttatg accattatcg tgctgttctg
     6721 gatcatggcg attatggacg gctggcgcgg tatcaaggag acgtggcctg cggtcgtggt
     6781 tgcgggcggc tcgtttgcca tcgctcagta ccttagctct aacttcattg ggccggagct
     6841 gccggacatt atctcttcgc tggtatcact gctctgcctg acgctgttcc tcaaacgctg
     6901 gcagccagtg cgcgtattcc gttttggtga tttgggggcg tcacaggttg atatgacgct
     6961 ggcccacacc ggttacactg cgggtcaggt gttacgtgcc tggacaccgt tcctgttcct
     7021 gacagctacc gtaacactgt ggagtatccc gccgtttaaa gccctgttcg catcgggtgg
     7081 cgcgctgtat gagtgggtga tcaacattcc ggtgccgtac ctcgataaac tggttgcccg
     7141 tatgccgcca gtggtcagcg aggctacagc ctatgccgcc gtgtttaagt ttgactggtt
     7201 ctctgccacc ggcaccgcca ttctgtttgc tgcactgctc tcgattgtct ggctgaagat
     7261 gaaaccgtct gacgctatca gcaccttcgg cagcacgctg aaagaactgg ctctacccat
     7321 ctactccatc ggcatggtgc tggcgttcgc ctttatctcg aactattccg gactgtcatc
     7381 aacactggca ctggcactgg cgcacaccgg tcatgcattc accttcttct cgccgttcct
     7441 cggctggctg ggggtattcc tgaccgggtc ggatacctca tctaacgccc tgttcgccgc
     7501 gctgcaagcc accgcagcac aacaaattgg cgtctctgat ctgttgctgg ttgccgccaa
     7561 taccaccggt ggcgtcaccg gtaagatgat ctccccgcaa tctatcgcta tcgcctgtgc
     7621 ggcggtaggc ctggtgggca aagagtctga tttgttccgc tttactgtca aacacagcct
     7681 gatcttcacc tgtatggtgg gcgtgatcac cacgcttcag gcttatgtct taacgtggat
     7741 gattccttaa tgattgtttt acccagacgc ctgtcagacg aggttgccga tcgtgtgcgg
     7801 gcgctgattg atgaaaaaaa cctggaagcg ggcatgaagt tgcccgctga gcgccaactg
     7861 gcgatgcaac tcggcgtatc acgtaattca ctacgcgagg cgctggcaaa actggtgagc
     7921 gaaggcgtgc tgctcagtcg acgcggcggc gggacgttta ttcgctggcg tcatgacaca
     7981 tggtcggagc aaaacatcgt ccagccgcta aaaacgctga tggccgatga tccggattac
     8041 agtttcgata ttctggaagc ccgctacgcc attgaagcca gcaccgcatg gcatgcggca
     8101 atgcgcgcca cacctggcga caaagaaaag attcagcttt gctttgaagc aacgctaagt
     8161 gaagacccgg atatcgcctc acaagcggac gttcgttttc atctggcgat tgccgaagcc
     8221 tcacataaca tcgtgctgct gcaaaccatg cgcggtttct tcgatgtcct gcaatcctca
     8281 gtgaagcata gccgtcagcg gatgtatctg gtgccaccgg ttttttcaca actgaccgaa
     8341 caacatcagg ctgtcattga cgccattttt gctggtgatg ccgacggggc gcgtaaagca
     8401 atgatggcgc accttagttt tgttcacacc accatgaaac gattcgatga agatcaggct
     8461 cgccacgcac ggattacccg cctgcccggt gagcataatg agcattcgag ggagaaaaac
     8521 gcatgattat ttccgcagcc agcgattatc gcgccgcagc gcaacgcatt ctgccgccgt
     8581 tcctgttcca ctatatggat ggtggtgcat attctgaata cacgctgcgc cgcaacgtgg
     8641 aagatttgtc agaagtggca ctgcgccagc gtattctgaa aaacatgtct gacttaagcc
     8701 tggaaacgac gctgtttaat gagaaattgt cgatgccggt agcactggca ccggtgggtt
     8761 tgtgtggcat gtatgcgcgt cgtggcgaag ttcaggcagc caaagcggcg gacgcgcatg
     8821 gcattccgtt tactctctcg acggtttccg tttgcccgat tgaagaagtc gcgccagcca
     8881 tcaagcgccc aatgtggttc cagctttatg tactgcgcga tcgcggcttt atgcgtaacg
     8941 cgctggagcg agcaaaagca gcgggttgtt cgacgctggt tttcaccgtg gatatgccga
     9001 caccgggcgc acgctaccgt gatgcgcatt caggtatgag cggcccaaat gcggcaatgc
     9061 gccgctactt gcaggcggtg acgcatccgc aatgggcgtg ggatgtgggc ctgaacggtc
     9121 gtccacatga tttaggtaat atctcagctt atctcggcaa accgaccgga ctggaagatt
     9181 acatcggctg gctggggaat aacttcgatc cgtccatctc atggaaagac cttgagtgga
     9241 tccgcgattt ctgggatggc ccgatggtga tcaaagggat cctcgatccg gaagatgcgc
     9301 gcgatgcagt acgttttggt gctgatggaa ttgtggtttc taaccacggt ggccgccagc
     9361 tggacggtgt actctcttcc gctcgtgcgt tgcccgctat tgcagatgcg gtgaaaggtg
     9421 atatcgccat tctggcggat agcggaattc gtaacgggct tgatgtcgtg cgtatgattg
     9481 cgctcggtgc cgacaccgta ctgctgggtc gtgctttcct gtatgcgctg gcaacagcgg
     9541 gccaggcggg tgtagctaac ctgctaaatc tgatcgagaa agagatgaaa gtggcaatga
     9601 cgctgactgg cgcgaaatcg atcagtgaaa ttacgcaaga ttcgctggtg caggggctgg
     9661 gtaaagagtt gcctacggca ctggctccga tggcgaaagg gaatgcggca tagtcgtttg
     9721 agccccctca ccctaaccct ctccctcagg gagtggggac cgttcggcgc tgtatgtact
     9781 ccctcactct gaaacgacac cgcactcttt ttttctccct cgcccctccg gggtgagggg
     9841 aaaaggccgc actgctcacc aattttctgc tatcctgccc ccgcactaag ggggcagtat
     9901 gctaaacatc gtactttacg aaccagaaat tccgccaaat actggcaata tcatccgtct
     9961 ttgcgctaat accggctttc gtctgcatat catcgaaccg atgggatttg cctgggacga
    10021 taagcgcctg cgccgcgcgg ggctggacta tcacgagttt accgccgtta cgcgtcatca
    10081 tgactttcgc gcgttcctcg aagcagaaaa tccccagcgc ctgtttgccc tcaccacgaa
    10141 aggtacgcct gctcacagcg ccgtaaacta tcaggatggc gattatctga tgttcggccc
    10201 ggaaacacgc ggcctgccag cgagcattct tgatgccctg cccgctgaac aaaaaattcg
    10261 cattccgatg gtgccggaca gccgcagcat gaacctgtcc aatgcggtgt cagttgtggt
    10321 gtatgaagcc tggcggcagc tgggatatcc gggagcgttg ttgagagatt agttactgta
    10381 tgccggatag atgattacat cgcatccggc acgatcacag gacattagat cccatcccca
    10441 tactcaaatg tatggttaat accgttgaaa tgctggtcca tatccattga tggcttatcg
    10501 ctgtctggtt taccgacaat acgagccgga acgccagcgg cggttgtatg cggcggcacc
    10561 ggttgcagca ccacggaacc tgcgccaatc ttcgcgccgc gaccaacttc aatattgccg
    10621 aggattttcg cgcccgcgcc aatcatcaca ccttcacgaa ttttcgggtg acggtcacca
    10681 ccagatttac ccgtaccgcc aagcgtcaca gattgcagaa tcgatacgtc gttttcaatt
    10741 accgccgttt cacccacaac gatgcccgtg gcgtggtcga gcataatgcc acggccaatt
    10801 tttgctgccg ggtgaatatc gacctggaac gtcacagaaa cctggttttg cagaaagatt
    10861 gccaatgcgc gacgcccctg attccacaac cagtgaccga tgcgataggc ctgcaaggca
    10921 tgaaaaccct tcaggtataa caacggggtt gagtatttat ccaccgccgg gtcgcgggta
    10981 cgcaccgcct gaatatcaca ggccgcagag gcgatcattt ccgggtcggc ggcgtaggct
    11041 tcttccacca cttcacggat agcaatagca ggcataattg gcgatgacag cttgttcgcc
    11101 agcatgtagc tcagtgcact gccaaggttt tcgtgcttga gtagcgtcgc gtggtaaaaa
    11161 ctggccagca ttggctcaca atccgccagc gttctggctt cggctttaat attgttccag
    11221 actatttcca gttcttcaca cgacattgct tactccacac gatgagataa tgaccggccc
    11281 gttctgcgcg ggccgggtca tagcggtaac aaaggttccc tggggttagt ggctgctgcg
    11341 ctcgtccttg cgtgcacgac ctaataacgt caatgctgcc tcgcgcgcgt tttttccgca
    11401 atataatact tgataaattt cctcggttat tggcatttca acgccgaagc gatgcgccag
    11461 ttcgcggact tctttcgtat tgcggtagcc ttccaccacc tgaccaatct tctcctgcgc
    11521 gctttgtaca tccatgccct gaccgagcat catgccaaaa cggcggttac gcgactggtt
    11581 gtcggtacag gtaagcacca gatcgcccag ccccgccatg cccataaagg tggcaggatc
    11641 ggcacccagc gccgcgccaa gacgcgacat ttcagccagg ccacgggtga ttagcgccgt
    11701 acgcgcattc gcgccaaaac cgataccgtc ggacatcccc gcgccaatgg caatgacgtt
    11761 tttcaccgca ccgccaagct gcacaccgat gaaatccggg ttgctgtaaa cgcggaaact
    11821 tttgccacag tgcagcaatt gttggagatc atcggcaaag gtctggtcgg tagaggccaa
    11881 cgaaatagcc gtcggtaaac ctgccgccag ttctttcgca aacgttgggc cagagataac
    11941 cgccagcgga atttgatcgc ctaaggcctc acgcgccacg tcctgtaaca gacgtccggt
    12001 ttccgcttcc agccctttgg tcgcccacac cagacgcgca tcaggacgca tcagcggttt
    12061 aatctggcgc agcacttcac caaagacatg gctgggtacg acgacgagaa tattacggct
    12121 ggctgccagc gcagtggcga gatcgctttc aagatggagc gtatcgggaa aaggcacatc
    12181 ggggagaaac gcggcgttac agcggtcgcg ttcaagcgtt gcgatatgtt cagggtcatg
    12241 gccccagagg acaacctcgt ggccatttct tgccagggta atggcaagag cggtgccgta
    12301 cgagccggca ccgatcacag tcattaaagc attacgttgg ttcatcaggc atcctgatgt
    12361 tcttcagtac attcgccagc ctgctgctgc aaatagttca tgaacagcgc atcgaagtta
    12421 accggcgcaa ggttcagttg cgggaatgta ccgcgggata ccatgctggt gatgcactca
    12481 cgagcatacg ggaacagaat gttcgggcag tatgcgccca ggcaatgtgc catctgggtg
    12541 ccttcgatac ccgcgatgga gaaaataccg ccctgctgaa cttcacacag gaacgcggtt
    12601 tcttcgccca gggaggccgt tacggtaaca cgcagtacca cttcgtatac gtcatctgcc
    12661 agttgggaag atgccgtatc cagatcaagt ttaacttctg gttgccaatc tttctggaaa
    12721 acgtgcggcg cgttcggcgc ttcgaaagag atatccttgg tataaatacg ttggatctgg
    12781 aaagtcattt cagtgttgtt ttgttctgac atgtgtagaa aacccttaag tgttgtcctt
    12841 aaatactgcg caatgtaatg ccaacgttca cacgttattt cagcagggga tccagtccac
    12901 cacgtgcatc caatgcatac aagtcatcac agccgccaat gtgctgtgcg tcaataaaaa
    12961 tctggggaac cgtggtgcga ccgctgcgtt tgatcatctc ttcacgcttg gcggcattgc
    13021 catcgatcgg caactcctga aaactcacgc ccttgctgct cagcagtgct tttgcacgat
    13081 ggcaatacgg gcaggtttct ttggtataga tttcaacatt ggccataatt tagctcctgt
    13141 ttttttaccc tgaaatttca tgttgcaggg cggctgcaaa atggagtgtc cccagaagct
    13201 tataacaata agtcgctggg ggcctaattg cagctaacgg cctgcatcat gaaagacgac
    13261 aggtaaatta tttgccgcgc accaaaggca agttttcgcc agcccagcca gcgacgcctt
    13321 ctttcagcac gaatacttgc gcaaaaccag ctttcgtcag tgcgtttgca ggctcctggc
    13381 actgcatgcc agaaccgtct accacgataa ccggtttgtc tttgtgcttc tcaagctcac
    13441 caacattgtt ggctttgatt tcgctcggca acagattgat agaacctgcg atatggcctt
    13501 tacggaagtc atcacgctga cgtaaatcca caaccacagc gtcttctttg ttgatcagac
    13561 gcgtagcttc accacgagta atcaccttca ctttcgaggt caggctctta aacgtagtca
    13621 caagaaccgc caccagtaac gcgatccagg cgatactcag tatgggatga cggccaacaa
    13681 attgcataat ttcttgcatg gggggtaaca actcccgacg tagtgattaa aaaaaccagg
    13741 aaaggagtat acctgcgcgg tgcggcaaat acagccagcg cgttaactgg aatgcaattt
    13801 tgcggggcac gacgaaaaaa aagcgcaaat ctggcatcgg cttgccgcga gcggtcgtat
    13861 attttgatct tcagaggcta ttttatcgat tcagctgtag taaaattacg caaattttga
    13921 ctcttgagta tgaggttgtc gcaatgtcgg tttctaaaaa acctatggta ctggtgattc
    13981 tggatggcta tggctatcgt gaagaacagc aggataacgc catttttagt gctaaaaccc
    14041 cggtaatgga tgcactgtgg gccaatcgtc cgcataccct gatcgacgct tccggtctgg
    14101 aagtcggtct gcctgaccgt cagatgggta actccgaagt aggccacgtt aacctgggtg
    14161 ccggccgcat cgtgtatcag gacctgactc gtctggacgt tgaaatcaaa gatcgcgctt
    14221 tctttgctaa tccagtgctg actggcgcgg tagataaagc gaaaaacgca ggtaaagcag
    14281 tacacattat gggtctgctc tccgcaggcg gcgtacacag ccacgaagat cacatcatgg
    14341 cgatggtaga actggcagct gaacgcggcg cagaaaaaat ctacctgcac gcattccttg
    14401 acggtcgcga caccccgccg cgcagtgctg aatcctcgct gaaaaaattt gaagagaaat
    14461 ttgctgcgct gggtaaaggc cgcgtagcgt ccatcattgg tcgttactac gcgatggacc
    14521 gcgataaccg ttgggatcgc gttgaaaaag cctatgacct gctgactctg gcgcagggtg
    14581 agttccaggc cgatactgcc gttgctggtt tgcaggcagc ttatgctcgc gacgaaaacg
    14641 acgagttcgt gaaagcgacc gttattcgtg cagaaggtca gccagatgcg gcaatggaag
    14701 acggcgatgc gctgattttc atgaacttcc gtgctgaccg cgcgcgcgaa atcactcgtg
    14761 cattcgtaaa cgcagatttc gacggcttcg cgcgtaagaa agtggttaac gtcgatttcg
    14821 tgatgctgac cgaatacgcc gctgacatca aaactgcggt tgcttaccca cccgcttccc
    14881 tggttaacac cttcggcgag tggatggcga aaaacgacaa aactcagttg cgtatttccg
    14941 aaaccgaaaa atatgcccac gttactttct tcttcaacgg tggcgtagaa gagtcgttca
    15001 aaggcgaaga tcgcattctg atcaactcac cgaaagtggc tacctacgat ctgcaaccgg
    15061 aaatgagctc cgcagagctg accgaaaaac tggttgcggc catcaagagc ggcaaatacg
    15121 acaccattat ctgtaactat ccgaacggcg acatggtagg tcacaccggg gtgatggaag
    15181 cggcggttaa agcggttgaa gcactggatc actgcgtgga agaagtcgcg aaagcggttg
    15241 agtccgtggg tggacaactg ctgatcaccg ctgaccacgg taacgctgaa cagatgcgcg
    15301 atccggcaac gggtcaggca cacacggcac acaccaacct gccagttccg ctgatttatg
    15361 ttggtgataa gaacgtgaaa gcggttgaag gcggcaaact ttctgacatc gcgccgacca
    15421 tgttgtcgct gatgggtatg gaaatcccgc aagagatgac tggtaagccg ctgttcatcg
    15481 tggaataatc cctccccatg aggggaaagg cgattaatac catgacacgg gccgtgaaac
    15541 cgcgcaggtt tgcaatcagg cccatcatct acgccagcgt tcttagcgct ggcgtattgt
    15601 tgtgcgcctt ttccgcccac gcggatgagc gtgaccaact caaatctatt caggccgata
    15661 tcgccgcaaa agagcgcgcg gttcgccaaa agcaacaaca acgcgcaagc ctgctcgcac
    15721 aattgaaaaa gcaggaagaa gcgatctctg aagccacccg taagctgcgc gaaacgcaaa
    15781 acacgctcaa tcaactcaat aaacagattg atgagatgaa cgcgtcgatt gccaaactgg
    15841 agcagcaaaa agccgcccag gagcgcagcc tcgccgcaca actggatgcc gcattccgtc
    15901 agggcgagca taccggtatt cagctaattc tcagcggtga agaaagccag cgtggacagc
    15961 gtttacaggc ttatttcggc tatctcaacc aggcgcgaca agaaaccatt gcccagttga
    16021 agcaaacgcg tgaagaagtc gccatgcagc gtgctgaact ggaagagaaa caaagcgagc
    16081 aacaaacgct gttatatgaa cagcgcgccc aacaggcgaa actgactcag gctctgaacg
    16141 agcgtaaaaa gacgctggcg gggctggaat cttccatcca gcaaggtcag cagcagctaa
    16201 gtgaactgcg tgccaacgaa tcccgtctgc gtaacagcat tgcccgtgcg gaagccgcgg
    16261 cgaaagcgcg tgctgaacga gaagcgcgtg aggcccaggc ggttcgcgac cgccagaaag
    16321 aagcgacgcg caaaggcacc acctataaac cgaccgaaag cgaaaaatcg ctgatgtccc
    16381 gaactggtgg cctgggtgcg ccgcgtggtc aggcattctg gccggttcgc gggccgacgc
    16441 tgcatcgcta tggtgaacag ctacagggcg aactacgctg gaaaggaatg gttatcggtg
    16501 cctctgaagg tactgaagtt aaagcgattg ccgacggtcg ggtgattctg gctgactggc
    16561 tgcaaggtta cggtctggtg gtggtggttg agcatggtaa aggcgacatg agcctttacg
    16621 gctataatca gagcgcactg gtgagcgttg gttcgcaggt tcgcgcgggc cagccaattg
    16681 cactggtggg cagcagtggc ggtcagggtc ggccttcact ctatttcgaa attcgccgcc
    16741 agggtcaggc ggtcaatcca cagccgtggt tgggaagata agttttgttt ccatttcgtc
    16801 gtaacgttct tgcatttgcc gctctgttgg cgctctcctc ccccgtactt gctggcaaac
    16861 ttgccatcgt cattgatgat tttgggtatc gcccgcacaa cgaaaaccag gtgctggcga
    16921 tgccctccgc tatctccgtc gctgtattac ccgattcacc gcacgccaga gagatggcga
    16981 ccaaagcgca taacagcggg cacgaagtgt tgattcatct cccgatggca ccactgagta
    17041 aacagccgct ggagaaaaat acgctgcgcc cggagatgag cagcgacgaa attgagcgca
    17101 ttattcgtag tgcggtcaat aacgtgccct atgccgtggg gatcaacaac cacatgggca
    17161 gcaagatgac ctctaacctg tttggtatgc agaaagtgat gcaggcgctg gagcgttaca
    17221 atctttactt cctcgacagc gtaaccatcg gtaataccca ggcgatgcgc gccgcgcaag
    17281 gtactggcgt gaaggtgatc aaacggaagg tgttcctcga cgattcgcag aacgaagcgg
    17341 acatccgtgt gcaatttaat cgcgcaattg atctggcgcg tcgcaacggt tcaaccattg
    17401 ccattgggca tcctcaccct tcaacggtac gcgtgctaca acagatggtt tataacctgc
    17461 cgccagacat tacgctggtg aaagccagca gcttgctgaa tgaaccgcag gttgatactt
    17521 ctacaccgcc gaaaaacgct gtgcctgatg caccgcgtaa tccgttccgt ggcgtgaagc
    17581 tgtgcaaacc gaagaaaccg atagaacctg tttatgctaa tcgcttcttt gaagtattaa
    17641 gcgaaagcat cagccagagc acgctgatcg tttacttcca gcatcagtgg caaggctggg
    17701 gcaaacagcc cgaagcggcg aagtttaacg ctagcgcaaa ttaaaggcgc tacgcgtaat
    17761 gcgatggtgc gaccgcgttt tgtcgcgcca ttgccataag cggaaagacc acagtaacgc
    17821 ctggtaaccg accttcacgc tgcgcacatt ggtaatcagg cgcttataca taccggaagt
    17881 aaatatctcg gcaatcatcc gttggcgggt aagaatatcc ggctctttgc gcaccgcatg
    17941 gcaaacacgc aatgcttcgt aagttatttg ctgatgaaat tccggataaa tcgtaatttt
    18001 gtcggcataa tttcgattta atttctccag caggcgggta atcttaatat agtgacgttg
    18061 ataattaagg tttttattcc cttgtctatg caaccgactc actgacgtat tatgcagata
    18121 atatttatat aatgattgct cggtatatcg cgcacgcagc gcgttaaaca tgaattctgt
    18181 tgtccagaca atatcctgat gatgtaatcc ggcaataaat ttaatgttat ttttaacaat
    18241 aacatcacgg cgataaaccc ccatccagac aacgtgagtc caacggcgcg aagaaagccc
    18301 catccgcagc cagtccgggc cggttaatac gccggttgag cgaaggcgat cgctggggat
    18361 ggattgccag gtttctcccg tttcacgaaa acaccagtca gcgttgcact gcgccacgtc
    18421 gaggtcatcc tctaacgcca tggtcatcag cgtttcgtac atggtgggat agacctcatc
    18481 gtcagcatcg acaaaagcga catatttgcc cgtcgccacc tcgatcccac gattacgcgc
    18541 taccgatgcg ccagcattcg cctgatgcaa caaacgaacg tgcggatagt tttctgcgta
    18601 atgctttgct atttcaacag aattatccgt tgaaccatcg ttaataataa tgatttccag
    18661 agcagtccag gtttgcgtaa ttaaagattc catacaagtg cggaaatcat cgcccgcatt
    18721 atataacgga ataataacac taagttta
//
LOCUS       DAZKHC020000080        17980 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZKHC020000080 DAZKHC020000000
VERSION     DAZKHC020000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.080"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            86..427
                     /gene="symE"
                     /locus_tag="ACHOSM_RS17045"
                     /old_locus_tag="pgaptmp_003409"
     CDS             86..427
                     /gene="symE"
                     /locus_tag="ACHOSM_RS17045"
                     /old_locus_tag="pgaptmp_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HGU4559858.1"
                     /translation="MTDTHSIAQPFEAEVSRANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRQVCKLSARKQKQVREF
                     IGVIAGKQKVA"
     gene            complement(474..1637)
                     /locus_tag="ACHOSM_RS17050"
                     /old_locus_tag="pgaptmp_003410"
     CDS             complement(474..1637)
                     /locus_tag="ACHOSM_RS17050"
                     /old_locus_tag="pgaptmp_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HGU4559859.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNGDLPH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVIIKEKYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRVHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPSLPEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALI"
     gene            complement(1685..2565)
                     /locus_tag="ACHOSM_RS17055"
                     /old_locus_tag="pgaptmp_003411"
                     /pseudo
     CDS             complement(1685..2565)
                     /locus_tag="ACHOSM_RS17055"
                     /old_locus_tag="pgaptmp_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(2662..2826)
                     /gene="yjiS"
                     /locus_tag="ACHOSM_RS17060"
                     /old_locus_tag="pgaptmp_003412"
     CDS             complement(2662..2826)
                     /gene="yjiS"
                     /locus_tag="ACHOSM_RS17060"
                     /old_locus_tag="pgaptmp_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HGU4559860.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            3003..4415
                     /gene="yjiR"
                     /locus_tag="ACHOSM_RS17065"
                     /old_locus_tag="pgaptmp_003413"
     CDS             3003..4415
                     /gene="yjiR"
                     /locus_tag="ACHOSM_RS17065"
                     /old_locus_tag="pgaptmp_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU4559861.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(4658..5602)
                     /locus_tag="ACHOSM_RS17070"
                     /old_locus_tag="pgaptmp_003414"
                     /pseudo
     CDS             complement(4658..5602)
                     /locus_tag="ACHOSM_RS17070"
                     /old_locus_tag="pgaptmp_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            6069..7301
                     /gene="mdtM"
                     /locus_tag="ACHOSM_RS17075"
                     /old_locus_tag="pgaptmp_003415"
     CDS             6069..7301
                     /gene="mdtM"
                     /locus_tag="ACHOSM_RS17075"
                     /old_locus_tag="pgaptmp_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HGU4559862.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLILPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            7342..8622
                     /gene="yjiN"
                     /locus_tag="ACHOSM_RS17080"
                     /old_locus_tag="pgaptmp_003416"
     CDS             7342..8622
                     /gene="yjiN"
                     /locus_tag="ACHOSM_RS17080"
                     /old_locus_tag="pgaptmp_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HGU4559863.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAIHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPIEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALLDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNDHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            8738..9889
                     /gene="yjiM"
                     /locus_tag="ACHOSM_RS17085"
                     /old_locus_tag="pgaptmp_003417"
     CDS             8738..9889
                     /gene="yjiM"
                     /locus_tag="ACHOSM_RS17085"
                     /old_locus_tag="pgaptmp_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HGU4559864.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            9899..10666
                     /gene="yjiL"
                     /locus_tag="ACHOSM_RS17090"
                     /old_locus_tag="pgaptmp_003418"
     CDS             9899..10666
                     /gene="yjiL"
                     /locus_tag="ACHOSM_RS17090"
                     /old_locus_tag="pgaptmp_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HGU4559865.1"
                     /translation="MAYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTNVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            10663..10920
                     /gene="ytiA"
                     /locus_tag="ACHOSM_RS17095"
                     /old_locus_tag="pgaptmp_003419"
     CDS             10663..10920
                     /gene="ytiA"
                     /locus_tag="ACHOSM_RS17095"
                     /old_locus_tag="pgaptmp_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HGU4559866.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGHTESVYCQQDGGWRCIAHYGISPR"
     gene            10985..11845
                     /gene="yjiK"
                     /locus_tag="ACHOSM_RS17100"
                     /old_locus_tag="pgaptmp_003420"
     CDS             10985..11845
                     /gene="yjiK"
                     /locus_tag="ACHOSM_RS17100"
                     /old_locus_tag="pgaptmp_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HGU4559867.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            11913..13091
                     /gene="yjiJ"
                     /locus_tag="ACHOSM_RS17105"
                     /old_locus_tag="pgaptmp_003421"
     CDS             11913..13091
                     /gene="yjiJ"
                     /locus_tag="ACHOSM_RS17105"
                     /old_locus_tag="pgaptmp_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4559868.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLAQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(13104..13658)
                     /gene="kptA"
                     /locus_tag="ACHOSM_RS17110"
                     /old_locus_tag="pgaptmp_003422"
     CDS             complement(13104..13658)
                     /gene="kptA"
                     /locus_tag="ACHOSM_RS17110"
                     /old_locus_tag="pgaptmp_003422"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HGU4559869.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            13908..14591
                     /gene="yjiH"
                     /locus_tag="ACHOSM_RS17115"
                     /old_locus_tag="pgaptmp_003423"
     CDS             13908..14591
                     /gene="yjiH"
                     /locus_tag="ACHOSM_RS17115"
                     /old_locus_tag="pgaptmp_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGU4559870.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            14588..15049
                     /gene="yjiG"
                     /locus_tag="ACHOSM_RS17120"
                     /old_locus_tag="pgaptmp_003424"
     CDS             14588..15049
                     /gene="yjiG"
                     /locus_tag="ACHOSM_RS17120"
                     /old_locus_tag="pgaptmp_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HGU4559871.1"
                     /translation="MTTQVRKNVMDMFIDGAHRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            15062..16234
                     /gene="iadA"
                     /locus_tag="ACHOSM_RS17125"
                     /old_locus_tag="pgaptmp_003425"
     CDS             15062..16234
                     /gene="iadA"
                     /locus_tag="ACHOSM_RS17125"
                     /old_locus_tag="pgaptmp_003425"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGU4559872.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            16299..17210
                     /gene="hypT"
                     /locus_tag="ACHOSM_RS17130"
                     /old_locus_tag="pgaptmp_003426"
                     /gene_synonym="qseD"
     CDS             16299..17210
                     /gene="hypT"
                     /locus_tag="ACHOSM_RS17130"
                     /old_locus_tag="pgaptmp_003426"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HGU4559873.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRYVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAADKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(17203..17595)
                     /gene="iraD"
                     /locus_tag="ACHOSM_RS17135"
                     /old_locus_tag="pgaptmp_003427"
     CDS             complement(17203..17595)
                     /gene="iraD"
                     /locus_tag="ACHOSM_RS17135"
                     /old_locus_tag="pgaptmp_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HGU4559874.1"
                     /translation="MMRQSRQAVLPEISGNKTSSLRKSICSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(17592..17675)
                     /gene="idlP"
                     /locus_tag="ACHOSM_RS17140"
                     /old_locus_tag="pgaptmp_003428"
     CDS             complement(17592..17675)
                     /gene="idlP"
                     /locus_tag="ACHOSM_RS17140"
                     /old_locus_tag="pgaptmp_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HGU4559875.1"
                     /translation="MENEHQYSGARCSGQAAYVAKHQECAK"
     gene            complement(17791..17933)
                     /locus_tag="ACHOSM_RS17145"
                     /old_locus_tag="pgaptmp_003429"
                     /pseudo
     CDS             complement(17791..17933)
                     /locus_tag="ACHOSM_RS17145"
                     /old_locus_tag="pgaptmp_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4214 a         4571 c         4494 g         4701 t
ORIGIN      
        1 atatccaggt atttagcgcg gtgcggatgt gcgccaacac acccgcaccg ctaatcacaa
       61 tcactattat cggagaatag cagttatgac tgacacgcat tctattgcac aaccgttcga
      121 agcagaagtc tcccgggcaa ataaccgtca attaaccgtg agttatgcga gccgctaccc
      181 ggattacagc cgtattcccg ccatcaccct gaaaggtcag tggctggaag ccgccggttt
      241 tgccaccggc acggcggtag atgtcaaagt aatggaaggc tgtattgtcc tcaccgccca
      301 acccgccgcc gccgaagaga gcgaactgat gcagtcacta cgccaggtgt gcaaactgtc
      361 ggcgcgtaaa caaaagcagg tgcgggaatt tattggggtg attgcgggta aacagaaagt
      421 cgcctgatac attgacccgg tagcaccagt gctaccgggt attattcgcc gcgttatatc
      481 aacgcgctct ccatctgttt ttcaaatgcc tgacgaatta atcccataat gtaaggaagt
      541 tgtgcgaggt cactgaaacc aatttccaca tcaccattac cccagcgccc aacatttgtc
      601 acatctttgg caataccttt cggatcgacc agttcatgaa actgcatatt aagcgacaat
      661 cgcagtcggc ttttttgcgg cacaacatca acaaaattcg tttccgcttt aaacgcaata
      721 tatagcttca atacttcctg cgtgatccct gcgtccaggc gcataacttc atcgcgaaga
      781 tgatcgaata acacccggct atgcgaacca tcagcaagaa aaggataatc actcaggctg
      841 taactggttt tgttttcagg ctgtccccga taaacagcca aaacctcttc aggaagagaa
      901 ggcgcttgcc agacctgaac cgcgagatcg gccagacgat cggcacgggc gcgaatggcg
      961 gcttcatccc atttttcgca ctgtccaaga ccgatattca aatagagcgg gctatgttta
     1021 aagccgtctt caatatcgcg tttttccgcg aaaggtctgt cactgtagcg ggagttatag
     1081 cgcgttaaag tgagattccc caacgtatgt aacagttctt tatgcacacg ctgccagtcg
     1141 cttcccagct cttcacgcca tttagccgac aaattttcgt tctgcggcat aatatgctca
     1201 atcgtaaact cttccacgcg ctctctgcgt ttatcgtttt ccagtcgtcg taaccagtag
     1261 ctgcgactac ggaagttata gagatcgcga acttttaatt cccgtttaaa ttcatcatcg
     1321 ttggggaaac gacgatatga aggcagattc agaaaatgta cctgaatact ttccagatat
     1381 ttttctttaa taatgacctt ataaaaggta gcaaacgtct tgttaagaga atttgtcgga
     1441 atcgcacata ctgcacggcg gaaaacataa gattcaatta aacgaattat gctcaggaaa
     1501 tcttcgtgag gcaaatcgcc atttttatag tcatgataaa gcgccagtaa gaaaggatac
     1561 gccacatcga cctttaactc gcgcaaatcc tgaaaagccg tagcaagcga tttgtcactt
     1621 tctttcccca atgccattgc acagtaatac tccgcgtaga tgtgaatatc ttcaaccagg
     1681 cgatctacgc ctttttcagc aacactctgg ctgcgggcat ggagtttaaa tgcctcatag
     1741 acttcatctg tccgagggat ctcccgtttt taccgtcaga taatgacgca taaaactgtc
     1801 aaaatattcg ctgtaacctt gctgaccaaa agcgacttcc atgggtcgcc agtgatcttc
     1861 atacaaccgg gtttgatgct ctggttcgag gcccatcaga ataaaattgc gcaccagatc
     1921 ggcctgactt aacgccttac cggtagagtt catactttca aaaatcagtt gcggattatc
     1981 ctgaccacga ttaagcgcca catcgacaat taataatttt gctaacccac gacataaggg
     2041 aatcaagtca tccccgagtt tggcaatttg ttcatcaaag aaagtaaagt tttccattat
     2101 tcgatgggaa tagttttctg gcattggtct ttgttttatt aacgccagca aactgtcttt
     2161 atcagtctcg gtcagcagta atttaaatcc tttctcgccg gactcataag gattgagtaa
     2221 ataataatta cgcaatttca ttgctgaaaa gccatcaaaa acttcgtctt cgccaagatg
     2281 acgcgataaa gcctcaatca gcaacattgc ggtcgtcagc ctttgttgac catcaatgac
     2341 cagtaacgga gaaataccag aaacctgata caacccctgc tcaatataaa caaccgaacc
     2401 gataaaatgc gctgatatat cgtcactctt tccggcacga atgatgtcgt cccaaagttg
     2461 ccgacattgt tgttctgtcc atgaataagt ccgttgataa atgggaataa caaactgctg
     2521 cgaacgtttt aaaaaatcga gtaggcgggc ttctgtagct ttcattctga cgtctttccg
     2581 tgaagcgatg atttcgcaat aataaccacc tccttgtgga ggttatagag aaatcctgtt
     2641 tatccgcaac gaactgcccc ctcactccac atcctccctg cgtaacccga tatccctcaa
     2701 ccgttcatca ctcatctgct gtaacacccg ccgggcctgc atttgccgcc gccaacgcct
     2761 caccgcctgc cagagttgca caagtctgat aaacggcgtt ttggctttgt tttcgtgaaa
     2821 ttccatctgc tgcctcctca tctttcagag gcttcatcat ccgtcttctg gcagttcaca
     2881 atacagatgc aaatattagt tttatttaac atacagacag gttaaaacaa catctgcatc
     2941 cggtttttta gcctcatctg taccggtttt tctctctgta tggtccaaat aaggaataca
     3001 gcatgacgcg ttatcaacat ctggcgactc tgcttgccga gcggattgag caagggctgt
     3061 atcgtcacgg ggagaaattg ccgtcggtgc gcagcttaag tcaggagcac ggcgtcagta
     3121 ttagcaccgt gcagcaggcg taccagacgc tggagacgat gaagctcatc actccgcagc
     3181 cgcgttcggg ttattttgtc gcacaacgta aagcccagcc gccagtgcca ccaatgacgc
     3241 gtccggtgca gcgcccggtg gaaattaccc agtgggatca ggtgctggat atgctggtgg
     3301 cgcatagcga cagttccatt gttccgttaa gcaaaagcac gccggatgtc gaaacgccca
     3361 gcctgaaacc gctgtggcgg gagctaagcc gggtggtgca gcataatctg caaaccgtgc
     3421 tcggttatga cttgttagcc ggtcagcggg tattgcgaga gcagattgcc cgcctgatgc
     3481 tcgacagcgg ctcggtggtc accgccgatg acatcattat caccagcggc tgccataact
     3541 cgatgtcgct ggcgttaatg gcggtatgta aaccgggcga tattgtcgca gtcgaatccc
     3601 cctgttatta cggttcaatg cagatgctgc gcggtatggg cgtgaaagtg attgaaatcc
     3661 caaccgatcc agaaactggc atcagcgttg aagcgctgga actggcgctg gaacagtggc
     3721 cgattaaagg catcattctg gtgccaaact gtaataatcc gctgggattt attatgccgg
     3781 acgcacgcaa acgggccgtt ctctctctcg ctcagcgtca tgatattgtg atttttgaag
     3841 atgatgtcta cggcgaactg gcgacggagt atccgcgccc gcggaccatt cattcctggg
     3901 atatcgacgg gcgagtgctg ctgtgcagct cgttcagtaa aagtattgca ccaggcctgc
     3961 gcgtgggttg ggtcgcaccg gggcgttatc acgataaact gatgcatatg aaatacgcca
     4021 tcagcagctt taatgtgccg tccacgcaaa tggcggcggc aacgtttgtg ctggaaggtc
     4081 actatcatcg ccatatccgg cggatgcggc agatctatca gcgcaatttg gcgctttata
     4141 cctgctggat acgggaatat tttccctgcg aaatctgtat tacgcgcccg aaaggcggat
     4201 ttttactgtg gatagaattg cctgaacagg tcgatatggt ctgcgtcgcg cggcagcttt
     4261 accgcatgaa aatccaggtg gcagcaggct cgattttctc ggcttccggc aaataccgta
     4321 attgtctgcg catcaactgc gctttgccgc tcagcgaaac ctatcgcgaa gcactaaagc
     4381 aaattggcga agccgtgtat cgggcaatgg aatgaattcg aaatagtggc gagccccctg
     4441 atttcctctc acgaatcgcg tgtgactcgt tttatctttg cagggtcatc gccacgttcc
     4501 ggcatggctt aggcaaagtg cgcttcgttc atgccggatg ccatgtgaac gccttaccag
     4561 gtctacaaaa tattaaaagt tcaatatatt taatggatta tgtagacctg ataagcgtag
     4621 cgcatcaggc tgctttgcgt ttgtcatcag cctctaatta gtattcgatc catggttcgc
     4681 gcttgctttc aggcaagggc tgctctaatg cctgcatttg ctctgccgaa agtccggtaa
     4741 tcttttgtat ccattcagga tctgccccat tctgcaacaa caatcggagg aggcgctgtt
     4801 ccccttcttc tttccctttc tccctcccta acagccatcc atcattatga attcgctctg
     4861 caatcgtcat tattcgctcc ctgtgttgtg gcattcgacg ggtaagttcg ctgataaact
     4921 cattaaaacg cgcttcatcg ccagtcagta aaatgtaatt taacagcgca gttatctagc
     4981 tgtcattagc acactctgta accagtaata ctaccagttg gtcgataagt cccatcagat
     5041 cgcgctggcg aatatgcttt tggatcaact ccaacagggc gactctgcga tgctgcacaa
     5101 tctcgtcgtc tggcacgaca gtgacatcca ccagcgggaa cgctgcggta taaagcttcc
     5161 gtgcggtggt cgggtcagca aattcgtcca gccagcacag ggaccaggga taaggactac
     5221 ggctaccgtg ataaaacagc atcgggatga ccagcggtag cggccggcgt ttatcatgct
     5281 cgatatggcg ctgcatcacc gccatggaat agcgcatcag gcgaaaggcc atatgaatat
     5341 cctcacggct ctgatgttca atcaccacat agatatagcc atcaccttca cgggtctcta
     5401 ccgaccacag aatatcagag tgtagcgccc gcaatttttc atcgacaaaa ctggcggatt
     5461 ccagttttaa ggtgtcgaga tcgcacagtt cacgtaaatc tttgggtaag tggatctcca
     5521 taaaatcctg cgcggtgtca gggtgcgtga gaaaggattt aaataatgcg tcatgcggcg
     5581 tactggtcgt gaagtttgtc atggcgatcc gtcacctgtt gataagatgg cccgacttta
     5641 gcgactaaca aaaccattct cacccggtaa tttcccattc cccgaggtgc tttccaggct
     5701 atttttattc ttctgcaaac gatacatttt tccggaaaca ggctcgcatt ttacatttcc
     5761 tgtacccggc agatattccc ctttccaccg cactcttttg attaaccgca aattttaatt
     5821 acacttaagg tgtatatttt ctatgcaacc catcaattca agaggtgtaa tgtgctgatg
     5881 actatttgta atcgttatac attctgacct ggagtcagaa ggtatttctc tgtctgtgta
     5941 ttcacaggca gtgtggttga ttacatgaaa ttcagtacat ttgtccttct cgcctgcctt
     6001 tcgtcattac cgacggtatt gaatttcgtt ttccccattg gggttctccg gacaaggagt
     6061 tgtttgttat gccacgtttt tttgcccgcc atgccgccac gctgtttttc ccgatggcgt
     6121 tgattttgta tgactttgct gcgtatctgt cgacggacct gatccagcca gggatcatta
     6181 atgtggtccg tgattttaat gccgatgtca gtctggcccc ggctgccgtc agtctctatc
     6241 tcgctggagg catggcattg cagtggctgc tggggccgct ttccgacagg attggccgca
     6301 ggccggtgct gattaccggg gcgctaatat ttacccttgc ctgcgctgcg acaatgttca
     6361 caacgtctat gacacagttt cttatcgcgc gtgcaattca gggcaccagt atctgtttta
     6421 ttgccaccgt tggttatgtc acggtgcagg aggcgttcgg acagacaaaa gggatcaagt
     6481 tgatggcgat tatcacctcc atcgtactga ttgcgccgat tattggcccg ctttccggcg
     6541 cagctctgat gcactttgtg cactggaaag tcctttttgc catcattgcg gttatgggtt
     6601 ttatctcatt tgttggctta ctgctggcga tgccagagac ggtgaagcgc ggcgcggttc
     6661 catttagcgc caaaagcgtc ttgcgcgatt ttcgtaatgt cttttgcaat cggctgttcc
     6721 tctttggcgc agcaaccatc tctttaagct atatcccaat gatgagttgg gtggctgtct
     6781 cgccggtgat cctgatcgac gcaggcggct taacgacctc gcagttcgcc tggacacagg
     6841 ttccggtgtt cggcgcggtg attgtcgcga atgccatcgt ggcgcgtttc gttaaagatc
     6901 cgaccgaacc gcgctttatc tggcgtgccg tacccattca actggttggc ctcgcactgt
     6961 tgattatcgg caatctgctg tcaccgcacg tctggctgtg gtcggtgctg ggcaccagtc
     7021 tgtatgcttt cgggattggt ttgattctcc cgaccttatt ccgctttacg ctgttttcca
     7081 ataacttacc gaaagggacc gtctccgcat cgctgaatat ggtgatcctg atggtgatgt
     7141 ctgtctcggt cgaaatcggc cgctggctgt ggtttaacgg cggtcgcttg ccgtttcatc
     7201 tgttagccgt tgtggcaggc gttatcgtcg ttttcaccct ggccggattg cttaatcgcg
     7261 tgcgccaaca tcaggctgcc gagctggcgg aggagcggtg atttttgcgc gatcctgccg
     7321 tcaggctcta ttcttaacat tatgaataaa ctcattgaac tcagacgcgc caaaatgttg
     7381 gcgctctctt tactgcttat cgccgctgct acctttgtcg ttacgctgtt tttgccgccc
     7441 aatttttggg tgagcggcgt gaaggcgatt gctgaagcgg cgatggtcgg cgcgctggcg
     7501 gactggtttg cggtggtggc gctgtttcgc cgcgtgccga ttccgatcat ttctcgtcat
     7561 acggcgatta ttccgcgtaa taaagaccgg attggcgaaa atctcggtca gttcgtgcag
     7621 gaaaaatttc tcgataccca atcgctggtg gcattgattc gacgtcacga accggcgttg
     7681 ttgattggca actggtttag tcagccggag aacgcccgcc gcgttggtca gcatctgttg
     7741 cagatcatga gcggttttct tgaactgacc gatgatgcgc gtattcagcg cctgcttaag
     7801 cgcgcgatcc accgggcgat tgataaggtt gatctttctg gcaccagtgc gttgatgctg
     7861 gagagtatga ccaaaaacga tcgtcatcag gtgctgctcg atacgctgat cgcacagttg
     7921 atcgcccttc tccagcgcga taaatcgcgc aagtttatcg cccagcagat tgttcgctgg
     7981 ctggagagtg agcatccact gaaagccaaa attctcccca tcgaatggct gggcgaacat
     8041 agcgcggagt tggtttctga cgcggtgaat tctttgcttg atgatatcag ccgcgatcgt
     8101 gcgcatcaga tccgccatgc atttgatcgc gccacttttg ccctgctcga caagttgaaa
     8161 aacgatcctg aaatggcagc gcgagccgat gccgtaaaaa gctatctgaa agaagatgaa
     8221 gcttttaacc gctatctcag tgaattgtgg ggggatttgc gggaatggct gaaagcggat
     8281 atcaacagtg aagattctcg cgttaaagaa cgtatcgcgc gggcgggtca atggtttggc
     8341 gaaacgttaa ttgccgatga tgccttgcgg gcgtcgttaa atgatcatct ggaacaagcc
     8401 gcgcaccgcg tcgcgcctga gttttctgca ttcctgacgc gccatatcag cgatacggta
     8461 aaaagctggg atgcgcggga tatgtcgcgg caaatagagt taaatatcgg caaagatctg
     8521 cagtttattc gtgtcaacgg tacgctggtt ggcggttgta ttgggctaat tttatatttg
     8581 ctgtcgcagc tcccggcctt gttccccctc ggcaattttt agaaatccat gaaaaactaa
     8641 gtggctaacc gcgagagaga tcaaataaac catactaaaa gaatggtaat aatgtggcct
     8701 cttttcatta tctcccgtgg tacggggaag gaaaatcatg tcacttgtca ccgatctacc
     8761 cgccattttc gatcagttct ctgaagctcg ccagaaaggc tttctcaccg tcatggatct
     8821 caaggagcgc ggcattccgc tggttggcac ttactgcacc tttatgccgc aagagatccc
     8881 gatggcagcc ggtgcggttg tggtttcgct ctgttccacc tctgatgaaa ccattgaaga
     8941 agcggagaaa gatctgccgc gcaacctctg cccgctgatt aaaagtagct acggcttcgg
     9001 caaaaccgat aaatgcccct acttctactt ttcggatctg gtggtcggtg aaaccacctg
     9061 cgacggcaaa aagaaaatgt atgaatacat ggcggagttt aagcctgttc atgtgatgca
     9121 gctgccgaac agcgttaagg acgatgcctc gcgtgcgtta tggaaagccg agatgctgcg
     9181 cttgcaaaaa acggtagaag aacgttttgg gcacgagatt agcgaagatg ctctgcgcga
     9241 tgccattgcg ctgaaaaacc gcgaacgtcg cgcactggct aatttttatc atcttgggca
     9301 gttaaatcct ccggcgctta gcggcagcga cattctgaaa gtggtttacg gcgcaacctt
     9361 ccggttcgat aaagaggcgt tgatcaatga actggatgca atgaccgccc gcgttcgtca
     9421 gcagtgggaa gaaggccagc gactggaccc gcgtccgcgc attttaatca ccggctgccc
     9481 gattggcggc gcagcagaaa aagtggtgcg cgcgattgaa gagaatggcg gctgggttgt
     9541 cggttatgaa aactgcaccg gggcgaaagc gaccgagcaa tgcgtggcag aaacgggcga
     9601 tgtctacgac gcgctggcgg ataaatatct ggcgattggc tgctcctgtg tttcgccgaa
     9661 cgatcagcgc ctgaaaatgc tcagccagat ggtggaggaa tatcaggtcg atggcgtagt
     9721 tgatgtgatt ttgcaggcgt gccataccta cgcggtggaa tcgctggcga ttaaacgtca
     9781 tgtgcgccag cagcacaaca ttccttatat cgctattgaa acagactact ccacctcgga
     9841 tgtcgggcag ctcagtaccc gtgtcgcggc ctttattgag atgctgtaag gagtggcagt
     9901 ggcatattcg attggcattg attccggctc aaccgccacc aaagggatct tactggcaga
     9961 cggcgtgatt acgcgccgtt tcctcgttcc aacacccttt cgcccggcaa cagcaattac
    10021 tgaagcctgg gaaactctgc gcgaagggtt agaaacaacg ccgtttctga cgctcaccgg
    10081 ctacggacgg caactggtgg attttgccga taaacaggtg acggaaatct cctgtcacgg
    10141 gctgggcgca cggtttcttg cgccagcaac gcgcgcagta atcgacatcg gtggtcagga
    10201 cagcaaagtg attcagcttg atgatgacgg taacctgtgc gatttcctga tgaatgacaa
    10261 atgcgcggcg ggcaccgggc gtttcctgga ggtgatctcg cgcacgcttg gcaccaacgt
    10321 cgaacagctc gacagcatta ccgaaaatgt cacgccgcac gccatcacga gtatgtgcac
    10381 agtatttgcc gaatcagaag tgatcagcct gcgctcagcg ggcgtcgcgc cagaagcgat
    10441 tctcgccgga gtgattaacg cgatggcgcg gcggagtgcc aatttcattg ctcgtctctc
    10501 ctgtgaagcg ccgattctgt ttactggtgg cgttagccac tgtcaaaggt ttacccacat
    10561 gctggaaagc catctgggaa tgccggtaca aactcaccct gatgcgcaat tcgctggtgc
    10621 aattggcgcg gcggtgatcg gtcagcggca gagaaaacgc gcatgaaaga gtatctattt
    10681 ttatttcact ccacggtcgg cgtcatacaa acccgcaagg cgttgcaggc agcgggcatg
    10741 acttttcgcg tcagcgatat tccccgtgat ttacgcggcg gctgcgggtt atgcatttgg
    10801 ctgacctgtc cgcctggcga ggaaattcaa tgggtgatcc ctgggcatac ggaatcagtt
    10861 tattgtcagc aggatggtgg ctggcgctgc attgcacatt acgggatttc tccgcgttaa
    10921 ggattcatta agatcgctac gtatacttct cgatatctta ttgatttgcc ttaaagggat
    10981 taccgtgaca aaaagtattt cgttaagtaa acgcatcttc gttattgtga ttctttttgt
    11041 cattgtggca gtgtgtactt tcttcgtgca atcttgcgcc cgtaaaagca atcatgcagc
    11101 atcatttcag aattaccacg ccaccattga cggcaaagaa attgcaggca taacgaataa
    11161 tatctcttcg ctgacctggt cggcacaaag caatactctt tttagcacta tcaacaaacc
    11221 tgccgccatt gttgaaatga ccaccaatgg tgatttgatc cgcacaattc ctctggattt
    11281 tgttaaggat ctcgagacga tcgaatacat tggcgataat cagtttgtca ttagcgatga
    11341 gcgcgattat gccatttatg tgatttcact gaccccaaac tcggaagtaa aaatcctcaa
    11401 aaaaataaaa atcccgctgc aggaatcccc aaccaattgc ggttttgaag gattggccta
    11461 ttctcgccag gatcacactt tctggttttt taaagagaaa aacccgattg aggtctacaa
    11521 agttaacggg ctattaagta gcaatgaatt gcacatcagc aaagacaagg cactacaacg
    11581 gcaatttact ttagatgatg tgtccggcgc agaatttaac caacaaaaaa atacgcttct
    11641 ggtgctgtct catgaatctc gcgcactcca ggaagtaacg ctggttggcg aggttatcgg
    11701 tgaaatgtca ctaaccaaag gcagtcgagg tttgtcgcat aacatcaaac aggccgaagg
    11761 ggttgcgatg gatgcctctg gtaatattta tatcgttagc gaaccaaatc gtttttatcg
    11821 ctttacccct caatcatcgc attaacaggc tggcatgctt cacctttttg ttattaactt
    11881 gttaacaaca aaaatgacaa acaaggttaa acatgccttc gtccacgcat cccgtagaac
    11941 gcttttcttt cagcaccgcg cttttcggga tgctggttct gaccttaggt atgggtttag
    12001 gccgcttcct ttatacgcct atgttgcccg tcatgatggc ggaaggatcg ttttcattta
    12061 gccagctctc gtggattgcc agcggcaact atgcgggata tctggctggc agtctgctat
    12121 tttcttttgg cgcatttcac cagccatcgc gcctgcgccc atttctgttg gcttctgccc
    12181 tggcgagcgg gttgttgatc ctcgcaatgg catggttgcc gccatttatt ctggtgttat
    12241 tgattcgcgt cctggcgggt gtcgccagcg ccggtatgct gatttttggt tcgacgctga
    12301 ttatgcagca cacgcgccat ccttttgtgc tggcagcttt gttttctggc gttggcattg
    12361 gcatcgcact gggcaatgaa tatgttctgg caggcctgca ttttgacctc tcctcgcaaa
    12421 cgttatggca aggcgccggg gcgctttctg gcatgatgct gattgcactt acgcttttaa
    12481 tgccctcgaa aaaacacgcc atcacaccaa tgccattggc aaaaacggag caacagataa
    12541 tgagctggtg gttactggct attctgtacg gcctggcggg atttggttat atcatcgtcg
    12601 cgacttacct gccgctcatg gcgaaagatg caggctcacc attgttaacc gcccatctct
    12661 ggacgttagt cggcttatca attgtgcctg gttgctttgg ctggctatgg gcagcaaaac
    12721 gttggggggc tttgccctgc ctgacggcga atttgctggc gcaggctatc tgtgtgctgc
    12781 ttactctcgc cagcgactcg cctctcttgc ttatcatcag cagtcttggt tttggcggta
    12841 cctttatggg cacgacatct ctggtgatga ctatcgcccg ccaactgagc gtaccgggaa
    12901 atctcaatct tttaggcttt gtgacgctca tttacggcat tgggcaaatc ctcggcccgg
    12961 cgctgaccag tatgctcggc aatggaacat cggcgcttgc cagcgccact ctctgcggcg
    13021 cggcggcgtt gtttattgct gcgcttatca gcacagtgca attatttaag ctacaagtgg
    13081 tcacttcatg agggaagttt cccttaccac tcaagaaact caaccgcaac cgttgatgtc
    13141 agccagaccc cgttttccgc ttgccagaaa gggaggcctc gtttcgccat ttcctgtgct
    13201 ttgacggtta aaataaccgg cgatccgtgt cttgctccca ctttgcgtgc cgttgcttca
    13261 tcagcagaga gatgtacgta gtgacgctca cctgcaatca gcccctgttt ttttatctcg
    13321 tcaaggaaac ggcttgccgt accgtggtag agaaactgtg gcggtgtttt ttcggcaaag
    13381 gaaatagcga cctgcgaagt cgaatgcccc tgaaccgcgc ggatgcacct accatcactg
    13441 gaataactaa aacgcttttt atcgctggtg gcaaccacag tatccagcaa tgcacgggtt
    13501 aaccgcttgc ccgctttttg ggcacaaagt atgagcttat cgatatccgc ccatccttca
    13561 cggtccagta cgataccaat cgcctccggt ttgtgtcgga ggacaaaact taaaaacttg
    13621 ctcgtatccg ccagctcttt ttcgttgtac tttgccatct cgttttcgtc cctggatttt
    13681 ctgtttttat ttacttatgt cattgtcact gggtttatga atttactgca actcaatcca
    13741 gcgcagaacc ttttccccat agcgtaattt ttccgctatg caaaaaacgc atagccagaa
    13801 acatacttgc attttcccca tacccacatg gctgataagg tttttcagct ccaggaattt
    13861 tcctggctca agcaacacac aaacacaaaa aaacaatggg gattgttatg acacaacaag
    13921 gggatgctgt agccggggag ctggcgacag aaaaagtggg cattaaaggg tatctggctt
    13981 tttttctcac cataatcttt ttttccggtg ttttttcagg aacagacagc tggtggcgcg
    14041 tttttgattt ttccgtactg aacggatcgt ttgggcagtt acctggtgcg aatggagcca
    14101 ccacctcatt tcgcggggcg ggtggcgcgg gggctaaaga tggctttctc tttgcgctgg
    14161 agctggcacc gtcagtcatt ctctctctgg ggattatttc aattaccgat ggtcttggtg
    14221 gattacgcgc cgcgcagcag ttaatgacgc ctgtgctaaa accgttgttg ggcattccag
    14281 ggatctgctc gctggcgctg atcgctaacc tgcaaaacac tgatgccgcc gccggtatga
    14341 ccaaagagct ggcgcaggaa ggtgaaatta ccgaacgcga taaagtgatt ttcgccgcat
    14401 atcaaaccag cggtagcgcc atcatcacca actacttctc ttccggcgtc gcggtgttcg
    14461 cctttctggg cacgtccgta atcgtacctt tagccgtcat cctggtgttt aaatttgtcg
    14521 gagccaacat tttgcgcgtc tggcttaact tcgaagaacg tcgcaaccca acgcaaggag
    14581 cgcaagcatg acaactcagg tccgcaaaaa cgtcatggat atgtttatcg atggtgcgca
    14641 ccgtggcttt actatcgcca cgaccaacct gctgccaaac gtggtgatgg cgtttgtcat
    14701 cattcaggcg ctgaaaatta ccggcctgct cgactgggtg gggcatatct gtgaacccgt
    14761 aatggcgctg tggggattac ccggtgaagc ggcaaccgtc cttttagccg cattaatgag
    14821 tatgggtggc gcagtcggtg ttgccgccag tctggcaacc gctggcgcat taaccggtca
    14881 cgatgtcacc gttttactgc ccgcgatgta tttaatgggc aacccggtgc aaaatgtcgg
    14941 tcgctgcctg gggacagccg aagtgaatgc caaatattac ccgcatatca ttacggtctg
    15001 cgtgattaac gcattgctgt cgatctgggt tatgcagctt attgtttaac aaggagttac
    15061 catgattgat tataccgcag ccggttttac cctcctgcag ggagcgcatt tgtatgcgcc
    15121 ggaagatcgg ggaatttgcg atgtcctcgt cgctaacggc aaaattatcg ccgttgccag
    15181 taatatccct tctgacattg taccggactg cacggttgtc gatctcagcg ggcagatcct
    15241 ctgcccaggt tttattgatc aacacgtcca tttgattggc ggtggcggcg aagcaggtcc
    15301 cacaacgcgc acgccggaag tggcgctaag tcgcctgacg gaagcgggcg tcacgtcagt
    15361 ggttggtctg ctgggcaccg actctatctc tcgccacccg gaatccctgc tcgccaagac
    15421 ccgtgcgctc aatgaagaag gcatcagcgc ctggatgctg accggcgctt atcatgtccc
    15481 ttcccgcacc attacgggtt ccgtggaaaa agacgtggcg attattgatc gtgtgattgg
    15541 cgtgaaatgc gccatctctg atcaccgttc tgccgcaccg gacgtttatc acctggccaa
    15601 tatggcggca gaatcccgcg ttggcggttt gctcggcggt aaacctggcg tcaccgtgtt
    15661 ccacatgggc gacagtaaaa aggcgttaca gcctgtctat gacctgctgg aaaactgcga
    15721 tgtgccgatc agcaagctgc tgccgaccca cgttaaccgc aacgtaccgt tgtttgagca
    15781 ggcgctggag ttcgcgcgca aaggcggcac catcgatatc accagcagca ttgacgaacc
    15841 ggtcgcccct gccgaaggta ttgcccgcgc cgttcaggcg ggtattccgc tggcacgcgt
    15901 caccctcagc tccgacggca acggtagcca gccgttcttc gatgacgaag ggaatttaac
    15961 ccatatcggt gttgcaggtt ttgaaacgtt gctggaaacc gtgcaggtgc tggtcaaaga
    16021 ctatgatttc agtatcagcg atgccctgcg cccgctcacc agtagcgtag ccggtttcct
    16081 taacctgagc gggaaaggcg aaattctgcc aggcaatgac gcagacttac tggtcatgac
    16141 gccagaattg cgcattgagc aggtatacgc tcgcggcaaa ctgatggtca aagacggcaa
    16201 agcctgcgtg aaaggaacgt ttgaaacggc ataaacggtt gtcagccgcc cgaatggacg
    16261 tagaatgccc gcaagggcgg ctgacagagt aaaacgtaat ggatgactgt ggtgcggttt
    16321 tgcataatat tgaaaccaaa tggctttatg attttctgac cctggaaaaa tgccgcaatt
    16381 tttcccaggc ggcagtcagt cgttacgtct cgcaaccggc attcagccgc cgcatccgcg
    16441 cgctggaaca ggcgattggc gttgaattgt ttaaccgcca ggtgacgccg ctgcaactct
    16501 cggaacaagg gaaaatcttt cattcgcaga tccgccatct gttgcaacag ttagaaagca
    16561 acctggcaga gctgcgtggc ggcagcgatt acgcgcaacg taaaatcaag atagccgctg
    16621 cacactctct ttccctcggt ctgttaccgt ccattatcag ccagatgccg ccgctcttta
    16681 cctgggcaat tgaagccatt gatgttgatg aagcagccga taaactgcgt gaagggcaaa
    16741 gtgactgtat tttttccttt cacgacgaag atttgctgga agcgccgttt gaccacattc
    16801 gcttatttga atctcaattg ttccctgtct gcgccagtga cgaacacgga gaagcacttt
    16861 ttaacctcgc gcagccacac tttccgttac tgaattacag ccgcaactcc tacatggggc
    16921 gattgattaa tcgcaccctg acgcgccaca gtgagttaag tttcagcacc ttttttgtct
    16981 cttcgatgag cgagctttta aagcaggttg ccctcgacgg ctgtgggatt gcctggctgc
    17041 cggagtacgc catccaacaa gaaattcgca gcgggcagct cgttgtactg aatcgggacg
    17101 aactggtgat cccgattcag gcctatgcat accgaatgaa tacccgcatg aatccagttg
    17161 ccgaacgctt ctggcgtgaa ctgcgcgagc tggagattgt acttagctga cattctcgag
    17221 cgtcgcacta cgccaaccgc cattatccca gaccagatca aacgttagcg gttcatcgtc
    17281 gtggtacagc attatctcaa ggcttatcgc cagcgtgtgg ctgtacgctt ctttcagatt
    17341 gacctgcaac gccgcaatcc gtggctcatg aaataacagt gcagaacgca gctgacggca
    17401 ataccagctc tggagatgtt tgtcgctctg gttatgcact tgccactcgg gcatgcctga
    17461 aacaagtaat gacggtaatg ccgaatgagg agaattaaaa agagtgagga gatcgctaca
    17521 tattgattta cgcagcgatg acgttttatt gcctgaaatt tcaggtaaaa cagcctgacg
    17581 tgattggcgc atcattttgc gcactcctga tgtttagcaa catatgcggc ttgccctgaa
    17641 caccgggcac cactgtattg atgctcattt tccatagcca ttcccttttg ttcattacat
    17701 ccatgtcagt ctgagacagg cctgaatcct gtcagcaaag tataaaacct tacttgccta
    17761 tgaatatcta ctgacttttc aggaaaaaag tcatgatttg ataaaaatca tcaaactcaa
    17821 aaaaaaccgc tagtatattc tgaatattcc gaatagttac gtagatgaaa atacttgtca
    17881 ccaccgctac ttaattccgg tgtgaaatta tttatttccg cataatcagc catgtattat
    17941 ttgaaacaat tagtttgtaa ataatataaa ttgataacat
//
LOCUS       DAZKHC020000081        17967 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZKHC020000081 DAZKHC020000000
VERSION     DAZKHC020000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.081"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..428)
                     /locus_tag="ACHOSM_RS17150"
                     /old_locus_tag="pgaptmp_003430"
                     /pseudo
     CDS             complement(1..428)
                     /locus_tag="ACHOSM_RS17150"
                     /old_locus_tag="pgaptmp_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            complement(439..3873)
                     /gene="tssM"
                     /locus_tag="ACHOSM_RS17155"
                     /old_locus_tag="pgaptmp_003431"
     CDS             complement(439..3873)
                     /gene="tssM"
                     /locus_tag="ACHOSM_RS17155"
                     /old_locus_tag="pgaptmp_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HGU4559876.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTASYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNQ
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLAQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            complement(3982..5394)
                     /gene="tssA"
                     /locus_tag="ACHOSM_RS17160"
                     /old_locus_tag="pgaptmp_003432"
     CDS             complement(3982..5394)
                     /gene="tssA"
                     /locus_tag="ACHOSM_RS17160"
                     /old_locus_tag="pgaptmp_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HGU4559877.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGHKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            complement(5399..6142)
                     /gene="vasI"
                     /locus_tag="ACHOSM_RS17165"
                     /old_locus_tag="pgaptmp_003433"
     CDS             complement(5399..6142)
                     /gene="vasI"
                     /locus_tag="ACHOSM_RS17165"
                     /old_locus_tag="pgaptmp_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HGU4559878.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWPRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            complement(6139..8952)
                     /gene="tssH"
                     /locus_tag="ACHOSM_RS17170"
                     /old_locus_tag="pgaptmp_003434"
                     /gene_synonym="clpV"
     CDS             complement(6139..8952)
                     /gene="tssH"
                     /locus_tag="ACHOSM_RS17170"
                     /old_locus_tag="pgaptmp_003434"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HGU4559879.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTAEARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESESESESESESEQDNTGAEPADEAGSEQPEETAE
                     TVSPVQRLAHLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVIT
                     DLPKWLGDTIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQL
                     AELLYGGRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLL
                     DEVEKAHPDVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPET
                     MQEVLYPVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVI
                     IEPEVTDEIMSRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVD
                     GAFTADVEDAQNDESVTKDETVL"
     gene            complement(9005..9232)
                     /locus_tag="ACHOSM_RS17175"
                     /old_locus_tag="pgaptmp_003435"
                     /pseudo
     CDS             complement(9005..9232)
                     /locus_tag="ACHOSM_RS17175"
                     /old_locus_tag="pgaptmp_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9196..11046)
                     /gene="tssF"
                     /locus_tag="ACHOSM_RS17180"
                     /old_locus_tag="pgaptmp_003436"
     CDS             complement(9196..11046)
                     /gene="tssF"
                     /locus_tag="ACHOSM_RS17180"
                     /old_locus_tag="pgaptmp_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGU4559880.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTLGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEHLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCTPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            complement(11050..11463)
                     /gene="tssE"
                     /locus_tag="ACHOSM_RS17185"
                     /old_locus_tag="pgaptmp_003437"
     CDS             complement(11050..11463)
                     /gene="tssE"
                     /locus_tag="ACHOSM_RS17185"
                     /old_locus_tag="pgaptmp_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HGU4559881.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            complement(11470..12945)
                     /gene="tssC"
                     /locus_tag="ACHOSM_RS17190"
                     /old_locus_tag="pgaptmp_003438"
     CDS             complement(11470..12945)
                     /gene="tssC"
                     /locus_tag="ACHOSM_RS17190"
                     /old_locus_tag="pgaptmp_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HGU4559882.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSSFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLHQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            complement(12996..13220)
                     /locus_tag="ACHOSM_RS17195"
                     /old_locus_tag="pgaptmp_003439"
     CDS             complement(12996..13220)
                     /locus_tag="ACHOSM_RS17195"
                     /old_locus_tag="pgaptmp_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559883.1"
                     /translation="MTAHKNIPGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            complement(13255..13755)
                     /gene="tssB"
                     /locus_tag="ACHOSM_RS17200"
                     /old_locus_tag="pgaptmp_003440"
     CDS             complement(13255..13755)
                     /gene="tssB"
                     /locus_tag="ACHOSM_RS17200"
                     /old_locus_tag="pgaptmp_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HGU4559884.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            14452..14673
                     /gene="tssD"
                     /locus_tag="ACHOSM_RS17205"
                     /old_locus_tag="pgaptmp_003441"
                     /pseudo
     CDS             14452..14673
                     /gene="tssD"
                     /locus_tag="ACHOSM_RS17205"
                     /old_locus_tag="pgaptmp_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            14673..17417
                     /locus_tag="ACHOSM_RS17210"
                     /old_locus_tag="pgaptmp_003442"
                     /pseudo
     CDS             14673..17417
                     /locus_tag="ACHOSM_RS17210"
                     /old_locus_tag="pgaptmp_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            17429..17878
                     /locus_tag="ACHOSM_RS17215"
                     /old_locus_tag="pgaptmp_003443"
     CDS             17429..17878
                     /locus_tag="ACHOSM_RS17215"
                     /old_locus_tag="pgaptmp_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HGU4559885.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEISDLYAGKIIDLLQMKYFRKEKYNNIRWGVSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT         4053 a         4771 c         5050 g         4093 t
ORIGIN      
        1 agctgcaatg cccgctctgt gcggtagatc agtggtaggt cggattccgc aaaggagatt
       61 tgctgacgca gaatattgcc ggttcgggag ttaaaccagt ccagcatttc agtacgcgcc
      121 gggccgccct gcggccagaa cttatcctag tcgtggctta ccattgccgc cagcagttcc
      181 gcaccttcgc agaatcccgc cagtccctgt gtccgtgtcc gggcaagcgt atagtaggtg
      241 gcggtgtgta aatccacacc gttggcttta aaaatcgcca gcgccagcga cgccaccagt
      301 ttccagttca gctcaggctg tgacgggtgg ctggctttgt ttatttcctc gcggatggcg
      361 ctgaattccg gcagcccgcg cgggtcactg ccggtaacga tagtttgtgt cagtacgtta
      421 ctattcatcc ctgcctcctc agtacagtgt gtctgacaga ccaaactggc tgaacaaccc
      481 accgctgaac gggttatctt ccgtgtcggt atgcacacgg taggtcatcg cgccaccgtc
      541 cacgctaaag cgcacggtaa agttgccatc ctgtactccg gtcagttgtc cggccccgaa
      601 caggcggaac tgcgcccacg gcccgctgaa gctgatactg cgcggcgcgt tgccgctggt
      661 gccgatgagc gtcagcttac tttcgttgcc ttcgcgcatg ttgttaggcc agaccagatg
      721 ggcggtatag ttacggccct ggctgtaatc gactaactga ccgtcaaggt tcagtacact
      781 gcggcgttta ttgcctgaaa gcgataccgt ttccacggca aaggatgttc ccagaccgtt
      841 ctgtttgctg aagaagatgt cacggatttt ctgcgcagtt tccagttgcg caataatatc
      901 ttcgcgaata atgacgttgt tatcgccatc ctccagactc aggtcattat cgataaacag
      961 cttcaggttc tgctggtaga aggtatccag tatgccatcc ggtttaaaga agcgttcgaa
     1021 ggcatccagt gaggcatcct gtgcagaacg cggattaaac ggatagttat ttgccagttg
     1081 ctcgttaaac ggtttcacca cgctgtcgcg ccagtccact tccatataat gaacagcctc
     1141 caccatcacc acatgccagg cctggtcagc cagcctgcca acccagcggt tgagcggagc
     1201 aggcagcgtt tttgccatct ggcgggtggc gaatatcgga tcgctgctgt tctgagcaag
     1261 ccgtaactgc acggctttca gcgccgattt ccctggtaca ggcgcgttct ggattgccag
     1321 caggtagcgg tgcaactcgg tgagttgctg atacacggcc tgcatcgtgc tttctttgtc
     1381 tttctgtact gccagggtac tgttttccgg ggcgaattca tgcccgaggc gggtgagtaa
     1441 ctggtaatcc ggctctgcca gggcttcctc ccgttctttg gcagagagtt tttcagaaaa
     1501 gacgcctggc tgtgtgttgt cacgcagcac ggtcagcgcc cgctgcaaag gctggtcgcc
     1561 gctgataacc tgctccagcg ccccggtcag ttgtccgatg gactcaaaat tgcggatatt
     1621 cagattgtcc atcccggccc gccaggtggc ggtgtagtcg ctgatatact gctcggtcaa
     1681 ctggcgctgg atttccgcgc ggtcggcgtc actgtatttc acgctgcggg taaggttaag
     1741 tacccaggaa tccatcgccg tcagttcaac cagttcatcg cgctgtttta caaaatagct
     1801 ttgcaggccg taacgggtaa gaaactgtgg aacaaccagt ttgttgtcat cggcagatgt
     1861 aaacacctgg tcaaaggttg gccctacctg gtcacgcaga ttgaggtcgg caggaagaac
     1921 gcccagcgcc cgcgttttca ggctctggta aacccgctgg tagacaggca gtttgctcag
     1981 ttctttctgt gctgatacca cgggcttgtc atatggcgtc cagcggctga tggcgtcacc
     2041 gtcgcccgcc tgacgctctg cgtgccagtc agtatgagcc agcgcgtagt caagatggga
     2101 catcagttgt gcctggatat cgcgctgacc gtggaacttt tcgctccagc gttttgccat
     2161 atactgcttc accacctgat tgttacgtcc gcttttgtcc tccagcattc gcatcacgcg
     2221 cagcaccgcg agtttttctt cactctccgg cggcgcggcg ttcatcgctt tgaccagccc
     2281 gttaaacagc gagggcaggt aacgttgctc cagcagttgc agataggtct gttccacata
     2341 aggcccgata cgtcgtccct ggtacagtcc catatcggcc agacggctgc ggtcgcccca
     2401 gtcgccatag gccagtgtgg catcgcgtac cgggttcagg agcggcagtt gcaggttgcc
     2461 aaagtcatct tccccctgcg gaggcggcac gtccataaag gctttggcct gcttaagcac
     2521 ggtgatgccg gactgatagt taccgttgta atagtgatgc cagccggtga tgagcagcag
     2581 tgctgccacg ccgcctgtgg cagagaaaac cgtcaggcgg cgacgggaac gtatcagcca
     2641 ggcgcggctc tctgttgcca gattaggctc tgcgagtaat gcctgcggga acaggctgcg
     2701 ggtgaaataa gacgcggtgt ccaccagggg ccaggacgcc agtgggttat tgcctagccg
     2761 gtactggcgg gcggcagact gggtgaatat atcatccatc tgtccacgct gaagcgaaga
     2821 ggtgagatag acaccacgca gcatcacgtt catattttcg ccatcaagca gaccctcaag
     2881 cagtgacacc agcggttcac ggcttccctg catctggcgg gaaaaactga ataaagacgc
     2941 gcgggtgtga gtctgagcga ccatcagatc cggcaacgcc agattcattc gatccaccca
     3001 tgtctgccag aaagcattca actctgttcg ccagtcatca ttttcatggg cacggcgggt
     3061 gaacgtgacg cccagaatcg catcgcgatc ctgtctgttg agtgactgga acagggcggc
     3121 aaaaccctgt aataaatcaa gccgggtcag taccacgtaa accggtaact ggcagtgaag
     3181 atgctggcgt atatcctgta agcggctgcg cagcgtctgt aacagatgct cgcggcggcg
     3241 tttgtctgcg gtcagcagat cgggtaaatc gagtgtcaga ataatcccgt tcagcggctg
     3301 gcgcgcgcgc ttttctttca gccagccgag ggcatgttcc cacagacggc gatgcaggat
     3361 atccgcatca gcgggagcgc agagtgtgcc gtcgatatca aagatcaccg cctgtttacc
     3421 gacatggggc gtgaggtaca ggcgttgttc tgcgccgcgt gcgccctccg gggcataaat
     3481 aatgtcggac ggaaacccct cgcgcagcag tgtcgttttg ccactgcccg ccgggccgat
     3541 gaccatatac cacggcaact gccacaggaa acggcggttg tcgagatggc gttgcaggcg
     3601 cagcagccag cggtcaagat aacgctgctg ggcgttcagt tccacgctga gcggatcaac
     3661 ggcttcctcg cgctgttgct tttgcatttt ttcaagctgt tgtaggcgct tcatcacccg
     3721 gacggtaagc cacaccaggg caataatccc ccacgccgcc gttgccagcc agcggttcgc
     3781 cagtggtttg agccactgtt cttcatacag cgtccacgtc gggcctttcc accagatcca
     3841 caccagcaga aaaatccacg ccagtatcag cagccagaaa gcagagactt taaaccgggg
     3901 catcgccggt cgcagggctg atttgagtcc gctgagtagt cggggtgttg gtaatctgaa
     3961 cacaaaaact cctgttctct tttattgttc tgccgtgaat ttttgttcta gctgctcaag
     4021 cagggacggc tcccagtctg ccaccatcat ctgcaatccc atcctgaaca acatccggta
     4081 ctgctgctgc gcaagctgta ccagccccgc ctcttccgtc aactgtgcgg caaggtactg
     4141 gcggtgaaac tgatcgcggg gatcgccttc gggtagcatg tccagatatc gcagcgccgc
     4201 ctccagaccc tgctcagcaa aacaggctct ggctgccttc gattcctcac cgatttgcgc
     4261 aaccggtgca actttggcgt cgggcgaagc tgccagccat tgtttcgtct gctcactgat
     4321 aaacggtgtg tggtcattaa acagcagccc ggtaagctgg ggcagacgct caaggaagcg
     4381 gatgacctca tcgcggatgg cgtctgccac ctgtttgtga cccagacgca gcgccgtctg
     4441 tgcagaaagg cagtgaccat ccagccagta aggagccagc agtacgcttt tctccacctg
     4501 ctgccacagc gccatatcgg cactaccgag ctgagcatgg taatctgcca ccatatcggc
     4561 ggagaccgca gccagtggag tacggccatc gctttccgcc tggggcgtac tggtgatgtt
     4621 ctgccaaagc gcatggcggc gcagacggta gccctgcggt gaatccggct ggcgttcaca
     4681 taaaatggcc gccactttta acagcgtgtc gcgccaggct ttgtcgtcgt gactgtcgat
     4741 gacgatttgg ggagccggag cactgggttg cgtcacgccg ctttcagacg tggcggtggt
     4801 ctgtggtgaa ctaccggagg ccgccggagt ggggacagga gccggtgccg cacgattaaa
     4861 cgcacgctgg tacagggcaa aaagatcatc agtagcctgt gccagttccg gggcgttatg
     4921 tgactgccag cattgcgcca gttttgccag ttcccctgac agcgcatcgc gctgcgctgt
     4981 ggcggcgttc tgtgaaaaac cttcaatacc tgtttcaaag cgtttaatga cctggctggc
     5041 aaagcgtttt ttatgcgcca tattctgcgg cgcagccacc gcccagtaat gctccacata
     5101 cagcgccagc aggcgcaatg cgagatgagg atcaccggcg tgttgcaggg ttctcagcag
     5161 atgcgccagc agacgaaaat ccttactctc cgaagccaga atgaccagcc cccggcgctg
     5221 gagttccggg atatccagtt gactgtgggc cagcgatccc agcttgacga tttcgccgtc
     5281 gatatactcc cagttcgggt tgtcatcttt cagcgccgat gccagtttct cttccggcag
     5341 gttttccagc aggtgcgata tccagacatt cggatcgcgt aagtccattg ccatattttt
     5401 attctcccgc ccagtggcag gcatcgcgca ggggagcaat cgcccttgcc agcccgtcaa
     5461 tgttaaaggt cagctttccg gcggcattgt ctgcgccggt atctaccgtc agcgtttttg
     5521 cgccgaacag ttgcttaatt tcatcaatcc cggataaccc ccggctggac tccagcaggg
     5581 tgccgttctc gcgcacaaac cagtggctgc gcaatgcccg gttgtcggca ttcagagtga
     5641 cggcgatatc atggacatcc agagggtgca tcagcgccac ctgcatccgg gtgatattgt
     5701 ccacgcaact gaacatcagc accgggcgtg gcggaacatg accgatagcc ggggtggtta
     5761 tcaccaccgt cgggcgctcc cccggcacct gcgtcaccag cagttctgtg gtgttgccct
     5821 cgcggcgttt ttcctgctct gtggcgcggg gccaggcctc gcccacaaaa ccggctttga
     5881 ccagcgcccc gtcaaaccct gacggggaca gcggtgccag caggcggtca tagcagtcaa
     5941 gccgctccag cgccgcggac tcccgccgac aggactgcat ggcctgtaac gtggctgtcg
     6001 catccggcgg ggtcgtggtg gcgacggcga ccgccacacc ggccattgac gatgcttctg
     6061 cgggcgcggc agtggcgggt aagcccgtca tcaggcccgc gataaaccat cctgtctgcc
     6121 ggaaaacgcg caacctgctc ataaaaccgt ttcatccttt gtgacggact cgtcgttctg
     6181 agcatcttcc acgtctgccg taaatgcgcc gtccactgcc gacaaccgaa tccgggcaat
     6241 cgccgtgtta gccgccattt tctgcaacag cagcagcgag agcggcggta gcatgtcgcc
     6301 atcgatgacc gattccagca tcctcgcgcc gttttccgcg cgggtgacgc ggctcatgat
     6361 ttcgtccgtc acttccggtt ctataatcac ctccgcgcca aagcggctgc gcagcacgtt
     6421 atccagacgg gccagttttc cggcgataat ggtggcgagc gtctctttcg acagcggcag
     6481 atacggcacc acttccatac gcgccagcag ggcaggtttg aagaagtcgg ccagcaccgg
     6541 atacagtact tcctgcatgg tttccgggtc atcggcatgc tcgactatta cctggtagcc
     6601 gaggttggac gtcaggaaga agacgatatt tttgcagtca atcaggcggc cttcaccgtc
     6661 tgccatttcg cccttatcga acgcctggta gaacaggttg agcacatccg ggtgcgcttt
     6721 ttccacttca tcgagcagca ccaccgagta gggtttctgg cgaatcgctt cggtcagtac
     6781 gccgccttcg ccatagccga catagcccgg aggggagcca atcagtcgcg agacggtatg
     6841 tttctcctgg aactcggaca tattgatggt ggtcaggtac tggcgaccgc cgtagagcag
     6901 ctctgccagt tgcaggacgg tttcagtttt acccacgccg ctggggccag ccagcaggaa
     6961 cgcgcccagc gggcgtcccg gacggcgcag gtcggcgcgt gcggtcagca ggtgtttgtg
     7021 caggctggca atcgccaggt cctggccttt gatggtgtcg ccgagccatt ttggcaggtc
     7081 ggtgatgacc gacatttcat tctgcgacag gcggttaagc ggcacgccgg tccattcggc
     7141 aatcaccgcc gcaatctgtt ttttatcgac gtgcggggaa accagcaacc ggtcgttatg
     7201 cagggcgtcc agttcggcag tgagatgtgc cagacgttgt accggggaaa ctgtttcagc
     7261 ggtctcttcc ggctgttcgc tgccagcttc atcagccggt tcggcaccgg tattatcctg
     7321 ctctgactct gactctgact ctgactctga ctctgactct gactctgact ctgactctga
     7381 ctctggctgc gtatcctcca cggtatctgc gtccggcaac ggcgccgcat cgtcctctgc
     7441 cacgcccagt aactgctggc gcagcgcgat aatctcccgg accagcgtct gctgctggtg
     7501 ccaggccgct tccagttcat ccagcgccgt cagcgtttca tcatactgca ccagcacctc
     7561 ggtcatccgt gatgtgtcgg tacgcagtcc gatgcgaagc tcgcgctcaa gctggcgaat
     7621 ttccgcctcc tgctggtggc tcagagtggt cagcgccgag atttgcttcg gcggcgacga
     7681 caggttgatg gccacacggg cgcaggcggt atccagcaca tcaatcgctt tgtccggtaa
     7741 ctgacgcccg gagagataac gctcgcttaa tgtcgcagcg gcctgcaagg cgtcatcatc
     7801 aatcagcacg ccgtgagact gttcatagac cgccgacaga ccgcgcagaa taatggtggc
     7861 ttcggcagcg ttgggttcgc taaccttcac caactggaag cggcgcgaca gggcggcatc
     7921 tttttcgaag tattttttgt actcgctcca ggtggtggcg gcgatggttt tcagctcgcc
     7981 gcgcgccagc gccggtttga gcaggttgga gatatccagc ccgccctgct ggttgcccgc
     8041 gccaatcagg gtatgtgctt cgtcgataaa cagaatgacc ggcaccgggg aggaaatgac
     8101 ctccgccatc agccctttga aacgtttttc gaattcaccc ttcaccgacg ctccggcctg
     8161 caatgcgccc aagtcaaggg tcatgatatc ggtgtttttc agcttgtccg gcacctggcc
     8221 tgccacgatg cgcagcgcca gcccttcgat taaggcgctt ttacccacgc ctgcttcgcc
     8281 caccaccacc gggttgttct tgcggcggcg gcagagaatg tcgatcatca ggtcgatttc
     8341 gtggtcgcgg cacagtaccg ggtcaagcct gccgttacgg gcctctgcgg tcatgttttt
     8401 ggcgtagcgg gcaagcaggg tgtcagcagc gtccgtcagt gtgcctgcgc ctttaccgtc
     8461 tgcatccggc acgactgatt ccgccgactc ctgtgtccac tgcacaaagt cctgttgcag
     8521 acggtcacgg ttaatgccgg tcaacagccg ggcggcagca ggcggtatat aacgcagcgg
     8581 cgaatgcagc agggccagca gcaacacgcc gccacgcaat tcgctgtgct gcatctcagc
     8641 cgacgccagc agccagccct ctttcagcca ctcaaccagc atcggggaaa atgccgggta
     8701 gctgtccgca gaacgggcag ttgtgtagtt ctccaccgtc agcgcctggc gcaactcttc
     8761 catgccaata tcgccttggc gggtaataac ccgcaggtca ctgcgtggca tcgcgagcat
     8821 ctgaataagc acatggctga cggtaatttc cgctgcctgc tggctcatac attcagaggc
     8881 ggccatctcc agcgcctggc gggagaacag gttaagccgt tttaccagcg tgggaagatc
     8941 aatctggatc ataaacgtcc ttatcgaagt aatttgttta gctggtgcag gatgtcctgc
     9001 gtctggcatc ggcgctgaaa cgcagggttt cctgtgccgg atcgtcacgc agggataatt
     9061 ctggcgtccc cttttcaccc tttgccaggc ggtgcaacag ctccgccatc gcaaaaaaat
     9121 tgtaagagca ggcattatgg aaaacctgtg ccagcggatc attctgctct ttatctgccg
     9181 ggggatttcc cggactcaga taagaggatg ctgcccgatt ctttccgtcc atttccatgt
     9241 ctcctggctt tctgtgttca caacggtcag catgtggaat gagttaacgc tggcgtacag
     9301 cgacagaaaa tgcgaaagca ccgtaccgag cagatacatt tccccttcac agacaaacgg
     9361 ctccgggtgc agatacagcg tggaggccag tccccggacg ggaatacccg taaacaggcg
     9421 atcaacgggg cgggtctcca gacgctcaat ggcatccagc ttttgttgtg acagccgtgc
     9481 cgtctgcgga tgatggattg cgtggcgatc aaagttggcg atgacctgct tcagcgcgtc
     9541 cgtatccagt aatgacagat aattcaggtt catggcggag agtagggacc agtgcaggct
     9601 gccatcaatg accggccaga gtggttgcgt cgggcgggta atgttgcgaa atgaagcaac
     9661 agccgcattt ttaccggttg tgccggtgat atcgccggaa cgaatttgtg acggaagctc
     9721 acggttggta caggtcagcg ataccgaaac cacttcatta ctgagcagag attcgtctga
     9781 cggataactg ccatcagcat gtataaaggc aagggtatga tcaaagccgc gatggaacag
     9841 ggatgttttg gtccggtgat gccagtacac cacttcccgc ttccggctgt attccatctg
     9901 atggctgaaa ctttcaaagg ctggccactg gcgcgcggcc tgcgtcgaaa caggacgccc
     9961 cagatctttt ttcctgaaca tatcctggac ctggctcacc acactgttaa cggaaaatac
    10021 atcgtaatgt tgtgggtaat ggcggctggg aaccagcgga tagtctgccc gccggttgtc
    10081 cagcgtgatg gcttctgcat ggtggataaa taaattaatg gcaggtgtgc aatacaggcg
    10141 cagggaatcg cggcgcagcc ggatgtccac gggcaaaggg cgcgaaaaac gcagttgcag
    10201 ggtaaagaat tccgcctgca atccgtcagg caaagccgga caaccacaaa gatcaaaaaa
    10261 gagaaacgca tcggggtaac agaaatactc ctgaaggatc cgatagccgc tgtggacgtt
    10321 tttcggccag ggcagcatgg catcctgcgg ctcaaaaccg acagctttta gcataaactc
    10381 aggcaggcga atatgctgtt cacccacagt caggtcggca ccctgcaagt gttcgcagaa
    10441 ccataaataa agctggtcac gggtatagtt gtcgtcattg ccgagccaga agcgaagttt
    10501 gttcagatcc agcgtgcgga agtccgttcc cggtgcgacc gaaaaggtga tgttgataac
    10561 accattacgg gtcgtactgc ggttttcaat ctgctccagg cgaacgggca gcagccagat
    10621 atcccggcag agggtgaagg tacagggagg cggctcctcc tttttagcat cagagggcag
    10681 cacttcatca actctgattt ccccggcgtt ggttgtaaac tgctcattac gggggattaa
    10741 caccggtaca gaagacttat ccatatccgg cgtatattca ataagggtca ttgccggaac
    10801 cggacgcagg taattaggcc atagcatctt aataagcccg agcgtcagtt ctgggaattc
    10861 atcctcaatt ttctggcgga gattgccggt aagaaaagcc accccttcca gcaggcgttc
    10921 aatatccgga tccgcatctt tttcggccag gaagcgggcc agatggggtt tttccgttgc
    10981 cagcagcttg ctaagctggc gcaggtaatc gagttcttcc cggaaatagc gttcttcaaa
    11041 ttccatttgt cagtccaccc ggtaatgctg gctgccattc ggtgccatat caaattcaag
    11101 cacctcatgc gtttcgttaa atgtcaccca ggctgtaata tgaaacgtca tatccagcgg
    11161 gctggaggcg gatgaaacca ctgcctgcac ttcagcgtgt gagatccggg gttcatagcg
    11221 ttctatacat tgctcgatgg cccgactcat ggattcgcgg aagttttcag ccccttccgg
    11281 ttcatcaatg cccagctcag gcgcaccccg gcaactaccg gaccgggtat taagaatgtt
    11341 tcgtaggttc tggcgaatgg attgcctgag cgaatgaata ctgcctccct gccggtttct
    11401 ggccgaagag gcttccatac gctcaaaaag agaacctgtg gttctttccc gatccggtat
    11461 catggtgtgt tactccctgt ctaaccggcc aaccagggaa agctcaaaat ttgcccccat
    11521 aaatttaaaa tgaggtctca cgcttagcgc aacctggtac cagccgggtt cgccttctac
    11581 atccataacc tcaactttgg cagcgcgcag aggtttgcgg ctgcgcacgt ctgcaggcgg
    11641 gttttcctgg tcggcaacat actgacggat ccaggtatta agctctcttt ctaagtcgct
    11701 cctttctttc catgacccca gttgttcacg ctgcaacacc ttgatgtagt gcgctaaccg
    11761 attgatgatg aagagataag gaagctgcgt acccagctta tagttggttt ctgcatcttt
    11821 tcccggaaag tgcttcggtt tttgtaccga gtttgcagag aaaaaggctg cattatcaga
    11881 gtctttacgc atggtaaggg tgataaaacc ctcttcagcc agttcaaatt cgcggcgatc
    11941 ggtgaccagc acctcggttg ggattttggc ctgaatctgc cccatcgttt cgaacagatg
    12001 caccggcaga tctttcactg cgccgccgct ttgcggaccg ataatgtttg gacaccagcg
    12061 gtatttggca aaactgtctg cgatattgca tgccagcatc caggccgtat taccccacag
    12121 ataatcttcg tggttctggc tgacatcttc gtaatagtta aagttcttaa cagggttatc
    12181 tgttggtgaa tagggctggt gaagcaggaa gcggggtgcc gtaagcccta aatagcgcga
    12241 atcttccgag tcacgcaggg cgcgccattt ggtgtaggcc ggaccttcaa agatggcata
    12301 aagatctttg atattaggca gttcggtcca cgagttcagc cccataaact ccggggaaac
    12361 ggaggacagg aacggtgcgt gcgccattgc gcccacggca ctgacgtatt gcagcagttt
    12421 catgtcaggc gcggtatttt tgaactcata agcgcccagc acggcagcga tgggttcacc
    12481 gccaaactgt ccgaaactgg acgaataaac gtgcttataa aatccggact gaataatttc
    12541 tggcgcaaac tcaaagtctt ccagaagttc ctgcttggtg acatgaagca cgttgacctt
    12601 gatgttttca cggaaatcaa cgcgatccac catggttttc agtgagcgcc agaacgactc
    12661 gacctgctga aaggcaggcg catggatgat gacatccatc tggcggctga tgcgttcatc
    12721 aatatctgca atcatgctgt ccacggccag tttattgacc ggttcggcag atgcagtgga
    12781 ttgcaggatg ctggcaataa aggctgtcac accctggcgg gcaacgtcat aaccttccga
    12841 accgggctga atacgggtct gtgccatgat ttgatcaagc agcgacgatg cctgctcagg
    12901 ctgcccggca tgactggaaa cgagttcctc ttcctgtaaa gacatactga aaatccttct
    12961 gttttagctg tgtttctgaa accggctttt cgccatcagt gaagtacgcg ctgccagtgc
    13021 gacgcgctct gctgaaactc acaaaaatgg ataatctcat ccaatagatc actgatacgg
    13081 cgatccagtg ttgtaatcag cgcagccaca ccatcctggt tgatttcaac ggacgggctg
    13141 aaacatacct ggtaaaccac ctgcagaatg tctgcaacag agaggtggta tgtcccgggg
    13201 atatttttgt gtgccgtcat gcctggataa ccctgtttaa tgaaatagtg gtggttattt
    13261 attgctcact agaccgattt ctttgagcaa ctgctcgcgg gactcttcat tttccagcaa
    13321 tgcctggagc tgggtacgga atgccggaag gttacccatc ggccccttca gtgcagtcag
    13381 tgcttcacgc agttccagca atttattcac ttccggaacc tgccgggcca cgctatctgg
    13441 tgaaaaatca tccagtgatt tgatattcag cgccacattc agttcatcgt ctgttgtgct
    13501 tcccttcagc gtggcgggga cagtaaaatt gaggccaatg gctgcttcag ccatcactgc
    13561 accgaaattg tgcttgttaa cggaaactgc ctgacgttca tccagtgggg atgtttcctg
    13621 cgtatttccg gtatcgccga caacgagcat gttaagcgga agttccactt cagccgtctg
    13681 gccatctgtt tttgggacat acgatatatt aatacgctcg cgaggcgcga cgctgccttc
    13741 aaattttttg ctcataaaag tgatccgtca tgttatctcg tacaggccat gaacacggca
    13801 tatttgtacc gcaaaaaaat atcggcaatg ccgccctaat ggcggtgaaa ataaaatcac
    13861 cactccttca ttaccccgca ttttacgctt caaaataaat aagaaaagga ctttttatcg
    13921 atcgtaaaga ggggtattct gcaagcgtta aatagattgt gtgaacctgt gtaattcgcg
    13981 acaggtgtat tatggcaata atttaaatat agagttttta tgtaacttat tggaatatat
    14041 aataatttcg ctaaacataa gctaatctaa tgattgcttg tttatatatt tgttttgtga
    14101 ttgattaagt gggtgttttt aaggaatatc tcaaaaatta tcaaaatgat aattcagaaa
    14161 ggataaatac aatatttatt catgattcag ctttggccat tttatggagg caatacggac
    14221 tttgaacagt tgggtatggt tatccagata tatattatta agttattgct gaataagaga
    14281 tttaatgatt ttaaaaatag tttttacgac aaccgtaaat tctagctcgg tttgtatgta
    14341 aaaaatactg aaaaatgatg tggttccgtg aagtgagctg catctgcctg tctgggatta
    14401 acggcaggtc attgtaagta gtaattaacc acgttaacaa ggagtttaca aatgccaaca
    14461 ccgtgttata tctccatcac tggtcagacc cagggaaata ttactgcagg tgcatttacc
    14521 gctgattctg tcggcaacat ctatgtgcag gggcacgaag acgagatgct ggtgcaggaa
    14581 ttcctgcata acgttactgt cccgaccgat cctcagtccg gtcagcctgc gggtcagcgt
    14641 gctcacaagc ctttcatttt taccgtggcg ctgagagggg aaggctgacg gcggagacct
    14701 cgcgcagcgg tgagacaacg cggtacagct atgatgaccc ggcgagtgag ctgccgacag
    14761 ggatacagga cgcgacgggc agtacaaaac agatggcatg gagccgttac ggtcaactgc
    14821 tggccttcac ggactgctcg gggtacacga cgcggtatga gtacgaccag tacggtcaac
    14881 aaatcgccgt tcaccgggaa gaaggcatca gcacttacag cagttataac ccgcgcggtc
    14941 aactggtcag tcagagggat gcgcaggggc gggagacccg ttatgagtac agcgccgcag
    15001 gcgacctgac agcaacggta tcaccggacg gtaaacgcag caccatcgaa tatgataaac
    15061 gcgggcgtcc ggtgagtgtg acggaaggcg gtctgacgcg cagcatgggg tatgacgctg
    15121 ccgggcgcat caccgtgctg accaacgaga acggcagcca gtccacgttc cggtatgacc
    15181 cggtcgacag gctgactgaa cagcgcggtt ttgacggccg gacgcaacgt taccagtatg
    15241 acctgaccgg aaaactcacg cagagtgaag atgaggggct tatcaccctc tggcattacg
    15301 atgcgtcgga ccgcatcacg caccgtacgg tgaacggcga cccggcggag cagtggcagt
    15361 atgatgagca cgggtggtta accaccatca gccataccag tgaaggccac cgtgtggcgg
    15421 tccactacgg ctatgacgat aagggccgcc tgacgggcga acggcagacg gtggagaacc
    15481 cggagacggg ggagatgctg tgggagcatg agacggggca cgcgtacagc gaacaggggc
    15541 tggcgacccg tcaggagccg gacggtctgc cgccggtgga gtggctgacg tatggcagcg
    15601 gttatcttgc ggggatgaag ctgggcggaa caccgctggt cgagtacacg cgggaccggc
    15661 tgcaccgtga gacggcccgc agcttcggcg gggaggcata tgaacttgcc accgcctgga
    15721 ataccagcgg ccagctccgg agcaggcacc tgaaccttcc gctgcttgac cgtgactacg
    15781 actggaacga caacggacag ctaatccgca tcagcggccc gcaggagagc cgggagtacc
    15841 gttacagcga cacggggagg ctgacgggcg tccacaccac ggcagcgaac ctggatatcg
    15901 atatcccgta tgcaacagac ccggcaggaa accggctgcc ggacccggaa ctgcacccgg
    15961 acagcaccct cacggcgtgg ccggataacc gcatcgcgga agatgcgcac tatgtctatc
    16021 gctacgatga atacggcagg ctggcggaga agacggaccg tatcccggaa ggggttatcc
    16081 ggatgcacga cgagcgcacc caccactatc actacgacag ccagcaccgc ctggtgttct
    16141 acacgcggat acagcacggc gaaccacagg tggagagccg gtacctctat gacccgctgg
    16201 gccgccggac gggaaaacgg gtatggcggc gggagcgtga cctgacgggg tggatgtcac
    16261 tgtcgcgtaa accggaggtg acatggtacg ggtgggacgg tgacaggctg acgacgatac
    16321 agaccggcac cacgcgtatc cagacggtat accagccggg gagcttcacg ccgctcctga
    16381 gaatcgaaac agagaatggc gaacaggcga aggcgcggca ccggagcctg gcggaggtgt
    16441 tgcaggagga cacgggtgtg acgctaccgg cggagctgtc ggtgatgctg gggaggctgg
    16501 agcgggaact gcgcgcggga gcggtgagtg cggagagtga ggcatggctt gcgcagtgcg
    16561 ggctgacggc ggaacagatg gccgcgcagc tggaggcgga atacatcccg gagaggaaac
    16621 ttcatcttta ccactgcgac caccggggac tgccgctggc gctcatcagc ccggaagggg
    16681 aaacggcgtg gcagggggag tatgacgaat ggggaaacct gctgggcgaa accagcgcgc
    16741 agcacctcca gcagtcactc cgtctgccgg ggcagcagta tgatgaggag tcggggctgt
    16801 actacaaccg caaccggtac tatgacccgt tgcagggaag gtatatcacc caggatccga
    16861 tagggctgag aggtgaatgg aatctgtata aatatccatt aaaccctgtt cggtttatag
    16921 attctttggg tttaaaattc catgttaacg gtgatccaag tgattttaat caggctgttg
    16981 agtatttaaa acaagattca caaatgaaag aaactataga ttttttatca agctctgaag
    17041 aaactataaa tatagagtat attgaaggaa ccaatgttag atttaattcg aataatatga
    17101 cgatatattg gaatagtaga gcatctttat tttgttcaac tgaattaaac tcgaaatcac
    17161 aatcgcctgc attaggtttg ggacatgagt ttgctcatgc gcagtattac cttcttgata
    17221 aggaaaattt catggctctt ttaagccgaa cagataagaa atatgagaat aaagaagagg
    17281 caagggttat tacaataatt gaatcgcgtg cggcgaaaac tttaggtgag tgtaccagag
    17341 gagctcatag tggactgcct ttttatcggg tggatggacc gttgcagacg atgaaaataa
    17401 cgggtacgcc agaatagtga ggttttttat gatgaaatat ttgatggttt tattgtctct
    17461 attttctggt tcggtattgg ggatggggcg agtgaatgaa ttgtgtggaa tagatagcgt
    17521 gaaaacgata gagataatta atcttccatc gtatgttacc acgttagttc cgcttagcaa
    17581 ggaaggtctt aatgaaatat acagatataa agtggtggtg aatgaaatta gcgatcttta
    17641 tgctggtaag ataattgatt tattgcaaat gaagtatttc agaaaagaaa aatataacaa
    17701 tataagatgg ggggttagta tcatttcgaa aggtaataat aaatgtgaaa tttattttga
    17761 tgcttttggt gaatgtggaa gtgtgaatgg cattaacgtg tgttttgaaa aaaatgaaat
    17821 gataggttgg attaaaaaag aaatcccttt gctgtcgcaa aaaataggtg ggctttaaat
    17881 ggtagggcaa tatttaaaga cagtataggt aactatttgt atggccatgt aaggtaaaag
    17941 aaaaggaata aaccatgagc ggaaaac
//
LOCUS       DAZKHC020000082        17731 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZKHC020000082 DAZKHC020000000
VERSION     DAZKHC020000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.082"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(71..1354)
                     /gene="gltA"
                     /locus_tag="ACHOSM_RS17220"
                     /old_locus_tag="pgaptmp_003444"
     CDS             complement(71..1354)
                     /gene="gltA"
                     /locus_tag="ACHOSM_RS17220"
                     /old_locus_tag="pgaptmp_003444"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HGU4559886.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKH"
     gene            complement(1560..1667)
                     /locus_tag="ACHOSM_RS17225"
                     /old_locus_tag="pgaptmp_003445"
     CDS             complement(1560..1667)
                     /locus_tag="ACHOSM_RS17225"
                     /old_locus_tag="pgaptmp_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559887.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2048..2452
                     /gene="sdhC"
                     /locus_tag="ACHOSM_RS17230"
                     /old_locus_tag="pgaptmp_003446"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2048..2452
                     /gene="sdhC"
                     /locus_tag="ACHOSM_RS17230"
                     /old_locus_tag="pgaptmp_003446"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU4559888.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2446..2793
                     /gene="sdhD"
                     /locus_tag="ACHOSM_RS17235"
                     /old_locus_tag="pgaptmp_003447"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2446..2793
                     /gene="sdhD"
                     /locus_tag="ACHOSM_RS17235"
                     /old_locus_tag="pgaptmp_003447"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HGU4559889.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2793..4559
                     /gene="sdhA"
                     /locus_tag="ACHOSM_RS17240"
                     /old_locus_tag="pgaptmp_003448"
     CDS             2793..4559
                     /gene="sdhA"
                     /locus_tag="ACHOSM_RS17240"
                     /old_locus_tag="pgaptmp_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HGU4559890.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4575..5291
                     /gene="sdhB"
                     /locus_tag="ACHOSM_RS17245"
                     /old_locus_tag="pgaptmp_003449"
     CDS             4575..5291
                     /gene="sdhB"
                     /locus_tag="ACHOSM_RS17245"
                     /old_locus_tag="pgaptmp_003449"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HGU4559891.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5592..8393
                     /gene="sucA"
                     /locus_tag="ACHOSM_RS17250"
                     /old_locus_tag="pgaptmp_003450"
     CDS             5592..8393
                     /gene="sucA"
                     /locus_tag="ACHOSM_RS17250"
                     /old_locus_tag="pgaptmp_003450"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HGU4559892.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8408..9625
                     /gene="odhB"
                     /locus_tag="ACHOSM_RS17255"
                     /old_locus_tag="pgaptmp_003451"
     CDS             8408..9625
                     /gene="odhB"
                     /locus_tag="ACHOSM_RS17255"
                     /old_locus_tag="pgaptmp_003451"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HGU4559893.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKSPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9719..10885
                     /gene="sucC"
                     /locus_tag="ACHOSM_RS17260"
                     /old_locus_tag="pgaptmp_003452"
     CDS             9719..10885
                     /gene="sucC"
                     /locus_tag="ACHOSM_RS17260"
                     /old_locus_tag="pgaptmp_003452"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HGU4559894.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10885..11754
                     /gene="sucD"
                     /locus_tag="ACHOSM_RS17265"
                     /old_locus_tag="pgaptmp_003453"
     CDS             10885..11754
                     /gene="sucD"
                     /locus_tag="ACHOSM_RS17265"
                     /old_locus_tag="pgaptmp_003453"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HGU4559895.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11858..12580)
                     /gene="mngR"
                     /locus_tag="ACHOSM_RS17270"
                     /old_locus_tag="pgaptmp_003454"
     CDS             complement(11858..12580)
                     /gene="mngR"
                     /locus_tag="ACHOSM_RS17270"
                     /old_locus_tag="pgaptmp_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HGU4559896.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12749..14665
                     /gene="mngA"
                     /locus_tag="ACHOSM_RS17275"
                     /old_locus_tag="pgaptmp_003455"
     CDS             12749..14665
                     /gene="mngA"
                     /locus_tag="ACHOSM_RS17275"
                     /old_locus_tag="pgaptmp_003455"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HGU4559897.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLDALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTKLKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIVG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            14683..17318
                     /gene="mngB"
                     /locus_tag="ACHOSM_RS17280"
                     /old_locus_tag="pgaptmp_003456"
                     /pseudo
     CDS             14683..17318
                     /gene="mngB"
                     /locus_tag="ACHOSM_RS17280"
                     /old_locus_tag="pgaptmp_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
BASE COUNT         4310 a         4406 c         4807 g         4208 t
ORIGIN      
        1 tttaggcata taaaaattaa cccgccattt gaacggcggg ttaaaatatt tacaacttag
       61 caatcaacca ttaatgcttg atatcgcttt taaagtcgcg tttttcatat cctgtataca
      121 gctgacgcgg acgggcaatc ttcataccgt cactgtgcat ttcgctccag tgggcgatcc
      181 agccaacggt acgtgccatt gcgaaaatga cggtgaacat ggaagacgga atacccatcg
      241 ctttcaggat gataccagag tagaaatcga cgttcgggta cagtttcttc tcgataaagt
      301 acgggtcgtt cagcgcgatg ttttccagct ccatagccac ttccagcagg tcatctttgg
      361 tgcccagctc tttcagaact tcatggcagg tttcacgcat tacggtggcg cgcgggtcgt
      421 aatttttgta cacgcggtga ccgaagccca tcaggcggaa agaatcattt ttatctttcg
      481 cacgacgaac aaattccgga atgtgtttaa cggagctaat ttcttccagc attttcagcg
      541 ccgcttcgtt agcaccaccg tgcgcaggtc cccacagtga agcaatacct gctgcgatac
      601 aggcaaacgg gttcgcaccc gaagagccag cggtacgcac ggtggaggta gaggcgttct
      661 gttcatggtc agcgtgcagg atcagaatac ggtccatagc acgttccaga atcggattaa
      721 cttcatacgg ttcacacggc gtggagaaca tcatattcag gaagttaccg gcataggaga
      781 gatcgttgcg cggataaaca aatggctgac caatggaata cttgtaacac atggcggcca
      841 tggtcggcat tttcgacagc aggcggaacg cggcaatttc acgatgacga ggattgttaa
      901 catccagcga gtcgtgatag aacgccgcca gcgcgccggt aataccacac atgactgcca
      961 ttggatgtga gtcgcgacgg aaagcgtgga acagacgggt aatctgctcg tggatcatgg
     1021 tatgacgggt caccgtagtt ttaaattcgt catactgttc ctgagtcggt ttttcaccat
     1081 tcagcaggat gtaacaaact tccaagtagt tagaatcggt cgccagctga tcgatcggga
     1141 aaccgcggtg cagcaaaata ccttcatcac catcaataaa agtaatttta gattcgcagg
     1201 atgcggttga agtgaagcct gggtcaaagg tgaacacacc ttttgaaccg agagtacgga
     1261 tatcaataac atcttgaccc agcgtgcctt tcagcacatc cagttcaaca gctgtgtccc
     1321 cgttgagggt gagttttgct tttgtatcag ccatttaagg tctccttagc gccttattgc
     1381 gtaagactgc cggaacttaa atttgccttc gcacatcaac ctggctttac ccgtttgtta
     1441 tttggctcgc cgctctgtga aagaggggaa aacctgggta cagagctctg ggcgcttgca
     1501 ggtaaaggat ccattgatga cgaataaatg gcgaatcaag tacttagcaa tccgaattat
     1561 taaacttgtc taccactaat aactgtcccg aatgaattgg tcaatacttc cacactgtta
     1621 cataagttaa tcttaggtga aataccgact tcataacttt tacgcattat atgcttttcc
     1681 tggtaatgtt tgtaacaact ttgttgaatg attgtcaaat tagatgatta aaaattaaat
     1741 aaatgttgtt atcgtgacct ggatcactgt tcaggataaa acccgacaaa ctatatgtag
     1801 gttaattgta atgattttgt gaacagccta tactgccgcc aggtctccgg aacaccctgc
     1861 aatcccgagc cacccagcgt tgtaacgtgt cgttttcgca tctggaagca gtgttttgca
     1921 tgacgcgcag ttatagaaag gacgctgtct gacccgcaag cagaccggag gaaggaaatc
     1981 ccgacgtctc caggtaacag aaagttaacc tctgtgcccg tagtccccag ggaataataa
     2041 gaacagcatg tgggcgttat tcatgataag aaatgtgaaa aaacaaagac ctgttaatct
     2101 ggacctacag accatccggt tccccatcac ggcgatagcg tccattctcc atcgcgtttc
     2161 cggtgtgatc acctttgttg cagtgggcat cctgctgtgg cttctgggta ccagcctctc
     2221 ttcccctgaa ggtttcgagc aagcttccgc gattatgggc agcttcttcg tcaaatttat
     2281 catgtggggc atccttaccg ctctggcgta tcacgtcgtc gtaggtattc gccacatgat
     2341 gatggatttt ggctatctgg aagaaacatt cgaagcgggt aaacgctccg ccaaaatctc
     2401 ctttgttatt actgtcgtgc tttcacttct cgcaggagtc ctcgtatggt aagcaacgcc
     2461 tccgcattag gacgcaatgg cgtacatgat ttcatcctcg ttcgcgctac cgctatcgtc
     2521 ctgacgctct acatcattta tatggtcggt tttttcgcca ccagtggcga actgacatat
     2581 gaagtctgga tcggtttctt cgcctctgcg ttcaccaaag tgttcaccct gctggcgctg
     2641 ttttctatct tgattcatgc ctggatcggc atgtggcagg tgttgaccga ctacgttaaa
     2701 ccgctggcat tgcgcctgat gctgcaactg gtgattgtcg ttgcactggt ggtttacgtg
     2761 atttatggat tcgttgtggt gtggggtgtg tgatgaaatt gccagtcaga gaatttgatg
     2821 cagttgtgat tggtgccggt ggcgcaggta tgcgcgcggc gctgcaaatt tcccagagcg
     2881 gccagacctg tgcgctgctc tctaaagtct tcccgacccg ttcccatacc gtttctgcgc
     2941 aaggcggcat taccgttgcg ctgggtaata cccatgaaga taactgggaa tggcatatgt
     3001 acgacaccgt aaaagggtcg gactatatcg gtgaccagga cgcgattgaa tatatgtgta
     3061 aaaccgggcc ggaagcgatt ctggaactcg aacacatggg cctgccgttc tcgcgtcttg
     3121 atgatggtcg tatctatcaa cgtccgtttg gcggtcagtc gaaaaacttc ggcggcgagc
     3181 aggcggcacg cactgcggcg gcggctgacc gtaccggtca cgcactgttg cacacgcttt
     3241 atcagcagaa cctgaaaaac cacaccacca ttttctccga gtggtatgcg ctggatctgg
     3301 tgaaaaacca ggatggcgcg gtggtgggtt gtaccgcact gtgcatcgaa accggtgaag
     3361 tggtttattt caaagcccgc gctaccgtgc tggcgactgg cggggcaggg cgtatttatc
     3421 agtccaccac taacgcccac attaacactg gcgacggtgt cggcatggct atccgcgcag
     3481 gcgtaccggt gcaggatatg gaaatgtggc aattccaccc gaccggcatt gccggtgcgg
     3541 gcgtactggt caccgaaggt tgccgtggtg aaggcggtta tctgctgaac aaacatggcg
     3601 aacgctttat ggagcgttat gcgccgaacg ccaaagacct ggcgggccgt gatgtggtgg
     3661 cgcgttccat catgattgaa atccgtgaag gtcgcggctg tgatggtccg tgggggccac
     3721 acgcgaaact gaaactcgat cacctgggta aagaagttct cgaatcccgt ctgccgggta
     3781 tcctggagct ttcccgtacc ttcgctcacg tcgatccggt gaaagagccg attccggtta
     3841 tcccaacctg tcactacatg atgggcggta ttccgaccaa agtgaccggt caggcgctga
     3901 cggtgaacga gaaaggcgaa gatgtggttg ttccggggct gtttgccgtt ggtgaaatcg
     3961 cttgtgtatc ggtacacggc gctaaccgtc tgggcggcaa ctcgctgctg gacctggtgg
     4021 tctttggtcg tgcggcaggt ctgcatctgc aagagtctat cgccgaacag ggcgcattgc
     4081 gcgatgccag cgagtctgat gttgaagcat ctctggatcg tctgaaccgc tggaacaata
     4141 atcgtaacgg tgaagatccg gtggcgattc gtaaagcgct gcaagaatgt atgcagcata
     4201 acttctcggt cttccgtgaa ggtgatgcga tggcgaaagg gcttgagcag ttgaaagtga
     4261 tccgcgagcg tctgaaaaat gcccgtctgg atgacacttc cagcgagttc aacacccagc
     4321 gcgttgagtg cctggaactg gataacctga tggaaacggc gtatgcaacg gctgtttctg
     4381 ccaacttccg taccgaaagc cgtggcgcac atagccgctt cgacttcccg gatcgtgatg
     4441 atgaaaactg gctgtgccac tccctgtatc tgccagagtc ggaatccatg acgcgccgaa
     4501 gcgtcaacat ggaaccgaaa ctgcgcccgg cattcccgcc gaagattcgt acttactaat
     4561 gcggagacag gaaaatgaga ctcgagtttt caatttatcg ctataacccg gatgttgatg
     4621 atgctccgcg tatgcaggat tacaccctgg aagcggaaga aggtcgcgac atgatgctgc
     4681 tggatgcgct tatccagctg aaagagaaag atcccagcct gtcgttccgc cgctcctgcc
     4741 gtgaaggtgt gtgcggttcc gacggtctga acatgaacgg caagaatggt ctggcctgta
     4801 ttaccccgat ttcggcactc aaccagccgg gcaagaagat tgtgattcgc ccgctgccag
     4861 gtttaccggt gatccgcgat ttggtggtag acatgggaca attctatgcg caatatgaga
     4921 aaattaagcc ttacctgttg aataatggac aaaatccgcc agctcgcgag catttacaga
     4981 tgccagagca gcgcgaaaaa ctcgacgggc tgtatgaatg tattctctgc gcatgttgtt
     5041 caacctcttg tccgtctttc tggtggaatc ccgataagtt tatcggcccg gcaggcttgt
     5101 tagcggcata tcgtttcctg atcgatagcc gtgataccga gactgacagc cgcctcgacg
     5161 gtttgagcga tgcattcagc gtattccgct gtcacagcat catgaactgc gtcagtgtat
     5221 gtccgaaggg gctgaacccg acgcgcgcca tcggccatat taagtcgatg ttgttgcaac
     5281 gtaatgcgta aaccgtaggc ctgataagac gcgcaagcgt cgcatcaggc aaccagtgcc
     5341 ggatgcggcg tgaacgcctt atccggccta caagtcatta cccgtaggcc tgataagcgc
     5401 agcgcatcag gcgtaacaaa gaaatgcagg aaatctttaa aaactgcccc tgacactaag
     5461 acagttttta aaggttcctt cgcgagccac tacatagaca agagctcgca agtgaacccc
     5521 ggcacgcaca tcactgtgcg tggtagtatc cacggcgaag taagcataaa aaagatgctt
     5581 aagggatcac gatgcagaac agcgctttga aagcctggtt ggactcttct tacctctctg
     5641 gcgcaaacca gagctggata gaacagctct atgaagactt cttaaccgat cctgactcgg
     5701 ttgacgctaa ctggcgttcg acgttccagc agttacctgg tacgggagtc aaaccggatc
     5761 aattccactc tcaaacgcgt gaatatttcc gccgcctggc gaaagacgct tcacgttact
     5821 cttcaacgat ctccgaccct gacaccaatg tgaagcaggt taaagtcctg cagctcatta
     5881 acgcataccg cttccgtggt caccagcatg cgaatctcga tccgctggga ctgtggcagc
     5941 aagataaagt ggccgatctg gatccgtctt tccacgatct gaccgaagca gacttccagg
     6001 agaccttcaa cgtcggttca tttgccagcg gcaaagaaac catgaaactc ggcgagctgc
     6061 tggaagccct caagcaaacc tactgcggcc cgattggtgc cgagtatatg cacatcacca
     6121 gcaccgaaga aaaacgctgg atccaacagc gtatcgagtc tggtcgcgcg actttcaata
     6181 gcgaagagaa aaaacgcttc ttaagcgaac tgaccgccgc tgaaggcctt gaacgttacc
     6241 tcggcgcaaa attccctggc gcaaaacgct tctcgctgga aggcggtgac gcgttaatcc
     6301 cgatgcttaa agagatgatc cgccacgctg gcaacagcgg cacccgcgaa gtggttctcg
     6361 ggatggcgca ccgtggtcgt ctgaacgtgc tggtgaacgt gctgggtaaa aaaccgcaag
     6421 acttgttcga cgagttcgcc ggtaaacata aagaacacct cggcacgggt gacgtgaaat
     6481 accacatggg cttctcgtct gacttccaga ccgatggcgg cctggtgcac ctggcgctgg
     6541 cgtttaaccc gtctcacctt gagattgtaa gcccggtagt tatcggttct gttcgtgccc
     6601 gtctggacag acttgatgag ccgagcagca acaaagtgct gccaatcacc atccacggtg
     6661 acgccgcagt gaccgggcag ggcgtggttc aggaaaccct gaacatgtcg aaagcgcgtg
     6721 gttatgaagt tggcggtacg gtacgtatcg ttatcaacaa ccaggttggt ttcaccacct
     6781 ctaatccgct ggatgcccgt tctacgccgt actgtactga tatcggtaag atggttcagg
     6841 ccccgatttt ccacgttaac gcggacgatc cggaagccgt tgcctttgtg acccgtctgg
     6901 cgctcgattt ccgtaacacc tttaaacgtg atgtcttcat cgacctggtg tgctaccgcc
     6961 gtcacggcca caacgaagcc gacgagccga gcgcaaccca gccgctgatg tatcagaaaa
     7021 tcaaaaaaca tccgacaccg cgcaaaatct acgctgacaa gctggagcag gaaaaagtgg
     7081 cgacgctgga agatgccacc gagatggtta acctgtaccg cgatgcgctg gatgctggcg
     7141 attgcgtagt ggcagagtgg cgtccgatga acatgcactc tttcacctgg tcgccgtacc
     7201 tcaaccacga atgggacgaa gagtacccga acaaagttga gatgaagcgc ctgcaggagc
     7261 tggcgaaacg catcagcacg gtgccggaag cagttgaaat gcagtctcgc gttgccaaga
     7321 tttatggcga tcgccaggcg atggctgccg gtgagaaact gttcgactgg ggcggtgcgg
     7381 aaaacctcgc ttacgccacg ctggttgacg aaggcattcc ggttcgcctg tcgggtgaag
     7441 actccggtcg cggtaccttc ttccaccgcc acgcggtgat ccacaaccag tctaacggtt
     7501 ccacttacac gccgctgcaa cacatccata acgggcaggg cgcgttccgc gtctgggact
     7561 ccgtactgtc tgaagaagca gtgctggcgt ttgaatacgg ttatgccacc gcagaaccac
     7621 gcactctgac catctgggaa gcgcagttcg gtgacttcgc taacggtgct caggtggtta
     7681 tcgaccagtt catctcctct ggcgaacaga aatggggccg gatgtgtggt ctggtgatgt
     7741 tgctgccgca cggttatgaa gggcaggggc cggagcactc ctccgcgcgt ctggaacgtt
     7801 atctgcaact ttgtgctgag caaaacatgc aggtgtgcgt accgtctacc ccggcacagg
     7861 tttaccacat gctgcgtcgt caggcgctgc gcgggatgcg tcgtccgctg gtcgtgatgt
     7921 cgccgaaatc cctgctgcgt catccgctgg cggtttccag cctcgaagaa ctggcgaacg
     7981 gcaccttcct gccagccatc ggtgaaatcg acgagcttga tccgaagggc gtgaagcgcg
     8041 tagtgatgtg ttctggtaag gtttattacg acctgctgga acagcgtcgt aagaacaatc
     8101 aacacgatgt cgccattgtg cgtatcgagc aactctaccc gttcccgcat aaagcgatgc
     8161 aggaagtgtt gcagcagttt gctcacgtca aggattttgt ctggtgccag gaagagccgc
     8221 tcaaccaggg cgcatggtac tgcagccagc atcatttccg tgaagtgatt ccgtttgggg
     8281 cttctctgcg ttatgcaggc cgcccggcct ccgcctctcc ggcggtaggg tatatgtccg
     8341 ttcaccagaa acagcaacaa gatctggtta atgacgcgct gaacgtcgaa taaataaagg
     8401 atacacaatg agtagcgtag atattctggt ccctgacctg cctgaatccg tagccgatgc
     8461 caccgtcgca acctggcata aaaaacccgg cgacgcagtc gtacgtgatg aagtgctggt
     8521 agaaatcgaa actgacaaag tggtactgga agtaccggca tcagcagacg gcattctgga
     8581 tgcggttctg gaagatgaag gtacaaccgt aacgtctcgt cagatccttg gtcgcctgcg
     8641 tgaaggcaac agcgccggta aagaaaccag cgccaaatct gaagagaaag cgtccactcc
     8701 ggcgcaacgc cagcaggcgt ctctggaaga gcaaaacaac gatgcgttaa gcccggcgat
     8761 ccgtcgcttg ctggctgaac ataacctcga cgccagcgcc attaaaggca ccggcgtggg
     8821 tggtcgtctg acccgtgaag atgtggaaaa acatctggcg aaatccccgg cgaaagagtc
     8881 tgcaccggca gcggctgctc cggcggcgca accggctctg gctgcacgta gtgaaaaacg
     8941 tgtcccgatg actcgcctgc gtaagcgtgt ggcagagcgt ctgctggaag cgaaaaactc
     9001 caccgccatg ctgaccacgt tcaacgaagt caacatgaag ccgattatgg atctgcgtaa
     9061 gcagtacggt gaagcgtttg aaaaacgcca cggcatccgt ctgggcttta tgtccttcta
     9121 cgtgaaagcg gtggttgaag ccctgaaacg ttacccggaa gtgaatgctt ctatcgacgg
     9181 cgatgacgtg gtttaccaca actatttcga cgtcagcatg gcggtttcta cgccgcgcgg
     9241 cctggtgacg ccggttctgc gtgatgtcga taccctcggc atggcagaca tcgagaagaa
     9301 aatcaaagag ctggcagtca aaggccgtga tggcaagctg acggttgaag atctgaccgg
     9361 tggtaacttc accatcacca acggtggtgt gttcggttcc ctgatgtcta cgccgatcat
     9421 caacccgccg cagagcgcaa ttttgggtat gcacgctatc aaagatcgtc cgatggcggt
     9481 gaatggtcag gttgagatcc tgccgatgat gtacctggcg ctgtcctacg atcaccgtct
     9541 gatcgatggt cgcgaatccg tgggcttcct ggtaacgatc aaagagttgc tggaagatcc
     9601 gacgcgtctg ctgctggacg tgtagtagtt taagtttcac ctgcactgta gaccgacgtg
     9661 gatagggtgt cggtctacgg tttaaaagat aacgattact gaaggatgga cagaacacat
     9721 gaacttacat gaatatcagg caaaacaact gtttgcccgc tatggcttac cagcaccggt
     9781 gggttatgcc tgtactactc cgcgcgaagc agaagaagcc gcttcaaaaa tcggtgccgg
     9841 tccgtgggta gtgaaatgtc aggttcacgc tggtggccgc ggtaaagcgg gcggtgtgaa
     9901 agttgtaaac agcaaagaag acatccgtgc ttttgcagaa aactggctgg gcaagcgtct
     9961 ggtaacgtat caaacagatg ccaatggcca accggttaac cagattctgg ttgaagcggc
    10021 gaccgatatc gctaaagagc tgtatctcgg tgccgttgta gaccgtagtt cacgtcgcgt
    10081 ggtctttatg gcctccaccg aaggcggcgt ggaaatcgaa aaagtggcgg aagaaactcc
    10141 gcacctgatc cataaagttg cgcttgatcc gctgactggc ccgatgccgt atcagggacg
    10201 cgagctggcg ttcaaactgg gtctggaagg taaactggtt cagcagttca ccaaaatctt
    10261 catgggcctg gcgaccattt tcctggagcg cgacctggcg ttgatcgaaa tcaacccgct
    10321 ggtcatcacc aaacagggcg atctgatttg cctcgacggc aaactgggcg ctgacggcaa
    10381 cgcactgttc cgccagcctg atctgcgcga aatgcgtgac cagtcgcagg aagatccgcg
    10441 tgaagcacag gctgcacagt gggaactgaa ctacgttgcg ctggacggta acatcggttg
    10501 tatggttaac ggcgcaggtc tggcgatggg tacgatggac atcgttaaac tgcacggcgg
    10561 cgaaccggct aacttccttg acgttggcgg cggcgcaacc aaagaacgtg taaccgaagc
    10621 gttcaaaatc atcctctctg acgacaaagt gaaagccgtt ctggttaaca tcttcggcgg
    10681 tatcgttcgt tgcgacctga tcgctgacgg tatcatcggc gcggtagcag aagtgggtgt
    10741 taacgtaccg gtcgtggtac gtctggaagg taacaacgcc gaactcggcg cgaagaaact
    10801 ggctgacagc ggcctgaata ttattgcagc aaaaggtctg acggatgcag ctcagcaggt
    10861 tgttgccgca gtggagggga aataatgtcc attttaattg ataaaaacac caaggttatc
    10921 tgccagggct ttactggtag ccaggggact ttccactcag aacaggccat tgcatacggc
    10981 actaaaatgg ttggcggcgt aaccccaggt aaaggcggta ctacccacct cggcctgccg
    11041 gtgttcaaca ccgtgcgtga agctgttgct gctactggcg ctaccgcttc tgttatctac
    11101 gtaccagcac cgttctgcaa agactccatt ctggaagcta ttgatgcagg catcaaactg
    11161 attatcacca tcactgaagg catcccgacg ctggatatgc tgactgtgaa agtgaaactg
    11221 gacgaagccg gcgttcgtat gatcggcccg aactgcccgg gcgttatcac tccgggtgaa
    11281 tgcaaaatcg gtatccagcc gggtcacatt cacaaaccgg gtaaagtggg tatcgtttcc
    11341 cgttccggta cactgaccta tgaagcggtt aaacagacca cggattacgg tttcggtcag
    11401 tcgacctgtg ttggtatcgg cggtgacccg atcccaggct ctaactttat cgacatcctc
    11461 gaaatgttcg aaaaagatcc gcagaccgaa gcgatcgtga tgatcggtga gatcggcggt
    11521 agcgctgaag aagaagcggc agcgtacatc aaagagcacg ttaccaagcc agttgtaggt
    11581 tacatcgctg gtgtgactgc gccgaaaggc aaacgtatgg gccacgcggg tgcgatcatt
    11641 gccggtggga aagggactgc ggatgagaaa ttcgctgctc tggaagccgc aggcgtgaaa
    11701 accgttcgca gcctggcgga tatcggtgaa gcactgaaaa ctgttctgaa ataaatatct
    11761 gtaataagaa atagccctcg ccgcttccct ctacaggaat ggcgaagggc tgtcggtttc
    11821 gacatggttg gccatcgtat gatggccttt tttgtgctta tcgcgatgat tttcgctgcg
    11881 ctatcagggt aaatttatag tcatcggtat taaaagcgtt gcggctatat tcaaacaccc
    11941 gaccatcaac taaatatcca cgcgatactt tttcaagaat cggctttgtc tggctgatat
    12001 taagcagacg gctcatctct tcggttggca tcagaggaat gatttcctgt tcgctacgat
    12061 cgataaccat tttcttcact tcttcaataa agtgatattt cgaattttcc atgacctgcc
    12121 aggtgagatc cgggaacaac gcaagcggca tccaggtttc ttccagcgcc attggctttt
    12181 gcttgcgata gcgcacgcgc ttcacatgcc acacacgatc ctgcgcggtg atttgtagct
    12241 gttgctgaag aaaatcgtca gccggaatca cttcaaatat caggacttca ctgtgtgtat
    12301 cgacgtgacg gtccgacagt ttttcatcaa aactggttaa ctgaaaaata tcgtaattga
    12361 cccgctcttc tttgacgtaa gtcccgctgc cctgaatgct ttcgaggatc tgctgctcga
    12421 ctagctggcg caaagcctga cgcaccgtaa cccggctgac gccaaactct gtttgtagcg
    12481 ctgattcagt gggtaacgca tcgccaggtt taagctcgcc acgcgcaatt tgttcacgaa
    12541 tgcgatcggc aatctgccgg tataagggct tgtgtcccat ttttagtatc tcattaatac
    12601 gaatttaacc attatgcccg ataaattcat tctgtaaata atacaaatac aatacaaata
    12661 atctcaatca agtgaaattg atcacataat ggtattgttt tatcgggcac actggcgcga
    12721 ctataaaaac gatcaagtga ggatcatgat gaatctgacg actctgaccc accgcgatgc
    12781 gttgtgtctg aatgcgcgct ttaccagccg tgaagaggcc atccacgcgt tgactcaacg
    12841 tcttgatgct ctggggaaaa tttccagtac tgagcaattt ctggaagaag tgtatcgccg
    12901 tgaaagcctt ggcccgaccg ccttaggtga agggttggct gtgccgcatg gcaaaactgc
    12961 tgcggtaaaa gaagcggcgt ttgcggtcgc cacactcagc gagccgcttc agtgggaagg
    13021 cgttgatggc ccggaagcag ttgatttagt ggtgctgctg gcgattcccc ccaatgaagc
    13081 gggtactacg catatgcaac tgctgacagc gctgaccacg cgccttgcgg atgatgagat
    13141 tcgggcgcgt atacagtcgg cgacgacgcc tgatgagttg ctctcggcgc tggatgacaa
    13201 gggaggcacg caaccttctg cctctttttc caacgcgcca actatcgtct gcgtaacggc
    13261 ctgtccggcg ggtattgctc acacctatat ggctgcggaa tatctggaaa aagccggacg
    13321 caaactcggc gtaaatgttt acgttgaaaa acaaggcgct aacggcattg aagggcgttt
    13381 aacggcggat caactcaata gtgcaaccgc ctgtattttt gcggctgaag tcgccatcaa
    13441 ggagagtgag cgttttaatg gcattcccgc gctttcagtg cctgttgccg agccgattcg
    13501 ccatgcagaa gcgttgatcc aacaagcgct taccctcaag cgtagcgatg agacgcgtac
    13561 cgtacagcaa gatacgcaac cggtgaaaag tgtcaaaacg aagctgaaac aggcactgtt
    13621 gagcggaatc tcttttgccg taccgttgat tgtcgcgggg ggcacggtgc tggcggtcgc
    13681 ggtattactg tcgcaaatct tcgggctaca agatctgttt aatgaagaaa actcctggct
    13741 gtggatgtac cgcaagctgg gcggcgggct gctcggaatt ttgatggtac cggtgctcgc
    13801 ggcctatacc gcctattctc tggcagataa accggcgtta gcgccaggct ttgcggctgg
    13861 acttgccgcc aacatgatcg gctccgggtt tctcggcgcg gtcgttggcg gattgatagt
    13921 cggttacttg atgcgctggg tgaaaaatca cttgcgtctt agcagtaaat tcaatggatt
    13981 cctgactttt tatctctacc cggtgctcgg tacgttggga gcgggcagtc tgatgctgtt
    14041 tgtggtggga gaacctgtcg catggatcaa taactcgctt accgcctggc tgaacggtct
    14101 gtcaggaagt aacgcgctgt tgctgggtgc cattctcggt tttatgtgtt cctttgacct
    14161 tggagggcca gtgaataaag ccgcttatgc attctgcctg ggcgcaatgg cgaacggcgt
    14221 ttacggcccg tatgccattt tcgcctccgt caaaatggtt tcggcattta ccgtaaccgc
    14281 ttccacgatg ctcgcaccgc gcctgtttaa agagtttgaa attgagaccg ggaaatccac
    14341 ctggctgtta gggctggcag gtattaccga aggggcgatc ccgatggcga ttgaagatcc
    14401 gctgcgggtt attggttcgt ttgtgctggg ctctatggta acgggcgcta ttgtcggtgc
    14461 gatgaatatc ggcctttcga cacccggtgc cggcattttc tcgctctttt tacttcatga
    14521 taatggcgcg ggcggtgtta tggcggcaat tggctggttt ggcgcggcat tggtgggggc
    14581 tgcaatctcg actgcaattc tcctgatgtg gcggcgtcac gcggttaagc atggcaacta
    14641 tctgactgat ggcgtaatgc cataaacaaa aaggaaacga cgatgaaagc agtatctcgc
    14701 gttcacatca ccccgcatat gcactgggat cgagagtggt atttcaccac cgaagagtca
    14761 cgtattctgc tggtcaataa tatggaagag atcctgtgcc gactggaaca ggacaacgaa
    14821 tacaaatatt acgtactcga cgggcaaacg gcgatcctcg aagattattt cgcggtgaaa
    14881 ccggaaaaca aagaccgtgt gaagaaacag gtagaagccg gcaagttgat tatcggcccc
    14941 tggtataccc agaccgatac cacgattgtt tctgcggaat ccatcgtccg taatctgatg
    15001 tacggaatgc gtgactgcct cgcgtttggc gagccgatga aaataggtta tttaccagat
    15061 tcctttggca tgtccgggca actgccgcat atctacaatg gatttggcat tacccgcacc
    15121 atgttctggc gcggatgttc ggagcgccac ggtactgata aaaccgagtt tttgtggcaa
    15181 agcagtgacg gtagcgaagt gacggcgcag gtgctgccgc tgggctacgc catcggtaag
    15241 tacttacctg ccgacgaaaa cggattacgt aaacgcctcg acagttattt tgacgtgctg
    15301 gaaaaagcgt ctgtaaccaa agagattttg ctgccgaatg gtcatgacca gatgccattg
    15361 cagcaaaata tcttcgaagt gatggataag ctacgtgaga tctaccctca acgtaagttt
    15421 gtgatgagcc gctttgaaga ggtatttgag aagatcgaag cgcagcgtga aagtctggcc
    15481 accctgaaag gtgaatttat cgatggcaaa tatatgcgcg tacatcgcac catcggttcc
    15541 acgcgcatgg atatcaaaat tgcccacgca cgtattgaaa ataagattgt taatctgctg
    15601 gaaccgctgg caacgctggc ctggacgttg ggttttgaat accaccacgg cttgctggag
    15661 aaaatgtgga aagagatctt aaaaaatcat gcccacgaca gtatcggttg ttgttgcagt
    15721 gacaaagttc atcgcgaaat tgtcgcccgc ttcgaactgg ctgaagacat ggcggataat
    15781 ctgctgcgtt tctacatgcg caaaattgcc gataacatgc cacagagtga tgccgacaaa
    15841 ctcgtgctgt ttaacctgat gccctggccg cgggaagaag ttattaacac cactgtgcgg
    15901 ctgcgcgcca gccagtttaa tctacgagac gatcgcggtc agcctgtacc gtattttatt
    15961 cgccatgccc gtgagatcga tccaggccta atcgatcggc aaatagttca ttacggtaat
    16021 tacgatccct ttatggagtt tgatatacag atcaaccaga ttgtcccttc tatgggctat
    16081 cgcacgcttt atatcgaagc gaatcagcct ggcaacgtga ttgcggcaaa aagtgacgct
    16141 gaagggatac tggaaaatgc tttctggcaa attgcgctca atgaggatgg ttctctgcaa
    16201 ctggtagata aagacagcgg tgtgcgctat gaccgggtat tgcaaattga agaaagctct
    16261 gatgatggtg atgaatatga ctattcaccc gcaaaagaag agtgggtaat gacttctgca
    16321 acagcgaaac cgcaatgcga gattactcat gaggcctggc agagcagggc tgttatccgc
    16381 tatgacatgg cggtgccgct caatttgttg gaacgaagcg tccggcaatc cacgggcaga
    16441 gtaggggtgg agatggttgt cactctgagt cataacagca gacgtattga tgtggatatc
    16501 aaccttgata accaggctga cgatcatcgc cttcgcgtcc tgatccctac atcttttaac
    16561 accgatagtg ttctggcaga tacccagttt ggttcgctaa cgcgccccgt gaacgacagt
    16621 gcaatgaaca actggcagca agaaggctgg aaagaagcgc cggttccggt atggaatatg
    16681 ctcaactatg tagccttaca ggaagggcgt aacggcatgg ctgtctttag cgaagggcta
    16741 cgtgaatttg aagtcatcgg tgaagagaag aaaaaacctt tgccattacg ttgctgcgcg
    16801 gcgtgggctt actggggaaa gaagatctgc ttttaaggcc tgggcggcct tcgggaatta
    16861 aaatgccagt cccggactca caactacgtg gtctgctttc ttgtcgccta agtttattga
    16921 gttataccgg tacgccaacc gccgctggtg tagctcagca ggcgcgagca tggctgactc
    16981 cagtacagtg ttacaacaaa atcccatggg atgcgatgaa gctcaacaaa gccggattca
    17041 acgtgccgga aagttatagt ttgttgaaaa tgcccccagt gggatgcctg ataagcgcac
    17101 ttaagaaagc tgaagaccga caagaagtga ttttacggct gtttaatccg gctgaatcag
    17161 caacctgtga tgcgactgtt gctttcagtc gcgaggtgat ttcttgctca gaaacgatga
    17221 tggatgaaca cattaccacc gaggaaaatc aaggttcaaa tctatcgggg ccttttttac
    17281 ccggccagtc acggacgttc agttaccggc ttgcctgaat agcaatcaaa ccgaagccac
    17341 atatgcgcgg ccagattgtt gacaaagggc gctttgttca tgccggatac ggcatgaacg
    17401 ctttattcgg tctacaaaag caggcaaatt caatatattg cagagatgat gtagacactg
    17461 gcaagcgtag cgcatcaggt aattttgcgt ttatcttcac tctcaagcca cgtatatgtg
    17521 gctttatttt taacaaaata ataaccctgg gtgagttaat tataatataa ttacaagtta
    17581 attaaatgtt aatatcggcg gggtggattt atgcctttat tagtaatcct gaaactctgc
    17641 gtcgtattag ccagtgacca aaaaaagaat taaggtcaac cgtgctgttt ttgcttcgtc
    17701 tctttttatc tttaattgcc aaccgaaact a
//
LOCUS       DAZKHC020000083        17604 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZKHC020000083 DAZKHC020000000
VERSION     DAZKHC020000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.083"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            75..1214
                     /gene="arnB"
                     /locus_tag="ACHOSM_RS17285"
                     /old_locus_tag="pgaptmp_003457"
     CDS             75..1214
                     /gene="arnB"
                     /locus_tag="ACHOSM_RS17285"
                     /old_locus_tag="pgaptmp_003457"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HGU4559898.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALKIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQCCGISRDALIEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1218..2186
                     /gene="arnC"
                     /locus_tag="ACHOSM_RS17290"
                     /old_locus_tag="pgaptmp_003458"
     CDS             1218..2186
                     /gene="arnC"
                     /locus_tag="ACHOSM_RS17290"
                     /old_locus_tag="pgaptmp_003458"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HGU4559899.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2186..4168
                     /gene="arnA"
                     /locus_tag="ACHOSM_RS17295"
                     /old_locus_tag="pgaptmp_003459"
     CDS             2186..4168
                     /gene="arnA"
                     /locus_tag="ACHOSM_RS17295"
                     /old_locus_tag="pgaptmp_003459"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HGU4559900.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4165..5055
                     /gene="arnD"
                     /locus_tag="ACHOSM_RS17300"
                     /old_locus_tag="pgaptmp_003460"
     CDS             4165..5055
                     /gene="arnD"
                     /locus_tag="ACHOSM_RS17300"
                     /old_locus_tag="pgaptmp_003460"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HGU4559901.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCSLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5055..6707
                     /gene="arnT"
                     /locus_tag="ACHOSM_RS17305"
                     /old_locus_tag="pgaptmp_003461"
     CDS             5055..6707
                     /gene="arnT"
                     /locus_tag="ACHOSM_RS17305"
                     /old_locus_tag="pgaptmp_003461"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HGU4559902.1"
                     /translation="MKSLRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPNFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYTLSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6704..7039
                     /gene="arnE"
                     /locus_tag="ACHOSM_RS17310"
                     /old_locus_tag="pgaptmp_003462"
     CDS             6704..7039
                     /gene="arnE"
                     /locus_tag="ACHOSM_RS17310"
                     /old_locus_tag="pgaptmp_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HGU4559903.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7039..7425
                     /gene="arnF"
                     /locus_tag="ACHOSM_RS17315"
                     /old_locus_tag="pgaptmp_003463"
     CDS             7039..7425
                     /gene="arnF"
                     /locus_tag="ACHOSM_RS17315"
                     /old_locus_tag="pgaptmp_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HGU4559904.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7419..7685)
                     /gene="pmrD"
                     /locus_tag="ACHOSM_RS17320"
                     /old_locus_tag="pgaptmp_003464"
     CDS             complement(7419..7685)
                     /gene="pmrD"
                     /locus_tag="ACHOSM_RS17320"
                     /old_locus_tag="pgaptmp_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HGU4559905.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7795..9150)
                     /gene="menE"
                     /locus_tag="ACHOSM_RS17325"
                     /old_locus_tag="pgaptmp_003465"
     CDS             complement(7795..9150)
                     /gene="menE"
                     /locus_tag="ACHOSM_RS17325"
                     /old_locus_tag="pgaptmp_003465"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HGU4559906.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9147..10109)
                     /gene="menC"
                     /locus_tag="ACHOSM_RS17330"
                     /old_locus_tag="pgaptmp_003466"
     CDS             complement(9147..10109)
                     /gene="menC"
                     /locus_tag="ACHOSM_RS17330"
                     /old_locus_tag="pgaptmp_003466"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HGU4559907.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10109..10966)
                     /gene="menB"
                     /locus_tag="ACHOSM_RS17335"
                     /old_locus_tag="pgaptmp_003467"
     CDS             complement(10109..10966)
                     /gene="menB"
                     /locus_tag="ACHOSM_RS17335"
                     /old_locus_tag="pgaptmp_003467"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HGU4559908.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10981..11739)
                     /gene="menH"
                     /locus_tag="ACHOSM_RS17340"
                     /old_locus_tag="pgaptmp_003468"
     CDS             complement(10981..11739)
                     /gene="menH"
                     /locus_tag="ACHOSM_RS17340"
                     /old_locus_tag="pgaptmp_003468"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HGU4559909.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11736..13406)
                     /gene="menD"
                     /locus_tag="ACHOSM_RS17345"
                     /old_locus_tag="pgaptmp_003469"
     CDS             complement(11736..13406)
                     /gene="menD"
                     /locus_tag="ACHOSM_RS17345"
                     /old_locus_tag="pgaptmp_003469"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HGU4559910.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLACADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13495..14790)
                     /gene="menF"
                     /locus_tag="ACHOSM_RS17350"
                     /old_locus_tag="pgaptmp_003470"
     CDS             complement(13495..14790)
                     /gene="menF"
                     /locus_tag="ACHOSM_RS17350"
                     /old_locus_tag="pgaptmp_003470"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HGU4559911.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAVVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVVDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14869..15174)
                     /gene="elaB"
                     /locus_tag="ACHOSM_RS17355"
                     /old_locus_tag="pgaptmp_003471"
     CDS             complement(14869..15174)
                     /gene="elaB"
                     /locus_tag="ACHOSM_RS17355"
                     /old_locus_tag="pgaptmp_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HGU4559912.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15229..15690)
                     /locus_tag="ACHOSM_RS17360"
                     /old_locus_tag="pgaptmp_003472"
     CDS             complement(15229..15690)
                     /locus_tag="ACHOSM_RS17360"
                     /old_locus_tag="pgaptmp_003472"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4559913.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15755..16672
                     /gene="rbn"
                     /locus_tag="ACHOSM_RS17365"
                     /old_locus_tag="pgaptmp_003473"
     CDS             15755..16672
                     /gene="rbn"
                     /locus_tag="ACHOSM_RS17365"
                     /old_locus_tag="pgaptmp_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HGU4559914.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16860..17604
                     /locus_tag="ACHOSM_RS17370"
                     /old_locus_tag="pgaptmp_003474"
                     /pseudo
     CDS             16860..17604
                     /locus_tag="ACHOSM_RS17370"
                     /old_locus_tag="pgaptmp_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
BASE COUNT         4163 a         4735 c         4426 g         4280 t
ORIGIN      
        1 aaatggcaaa atatacacaa cactctttat agcaaatata agtggacagg tattcaatgg
       61 cggaaggaaa agcaatgtca gaatttttgc ctttttcgcg accagcaatg ggcgtggagg
      121 aactcgctgc agttaaagag gttctcgaat ccggttggat cacaaccggt ccgaagaatc
      181 aggcgcttga gcaagccttt tgccagttga cgggaaatca gcatgccatc gcggtcagtt
      241 cagccaccgc cggaatgcat atcacgctaa tggcgttgaa aattggcaag ggcgatgaag
      301 tgattacgcc ttccctgacc tgggtttcaa ccctcaatat gatttccttg ttgggcgcaa
      361 cgccggtaat ggtggatgtc gaccgcgata cgctgatggt cacgcctgaa gctatcgagt
      421 cagccattac gccacgcact aaagccatca ttccggtgca ttatgccggt gcgccagcag
      481 atattgacgc cattcgcgcc attggcgaac gttacggcat cgcagttatc gaagatgctg
      541 cccatgccgt cggtacgtat tacaaagggc gacatattgg cgcaaaaggt accgctattt
      601 tttcatttca tgccattaaa aatattacct gtgctgaagg tggcctgatt gtaactgata
      661 atgaaaacct tgcccgccag ctacggatgc tgaaatttca cggtctgggt gtcgatgcct
      721 atgacagaca aacctggggc cgtgcaccgc aggctgaagt cttaacaccg ggctataagt
      781 acaatctgac cgatattaac gccgcgattg ccctgacaca gttagtcaaa ttagagcacc
      841 tcaacacccg tcggcgcgaa attgcccagc aatatcagca agcactggca gctctcccct
      901 ttcagccatt aagccttccc gcctggccgc acgttcacgc ctggcatctg tttattattc
      961 gtgtcgatga acaatgttgt ggtatcagtc gcgatgcgtt gatagaagcg ttaaaagaaa
     1021 gaggcattgg taccgggtta catttccgcg ccgctcacac acaaaaatat tatcgcgagc
     1081 gttttcccac gctgtcgtta ccgaataccg aatggaatag cgaacgcatc tgttcgttgc
     1141 cgctgttccc ggatatgact accgccgatg ccgaccgcgt catcacagcc cttcagcaac
     1201 tcgcaggaca ataagccatg tttgaaatcc accctgttaa gaaagtctcg gtggttattc
     1261 ccgtttataa cgagcaggaa agcttaccgg aattaatcag gcgcaccacc acagcctgtg
     1321 aatcgttggg gaaagagtat gagatcctgc tgattgatga cggcagtagc gataattccg
     1381 cgcatatgct ggtcgaagcc tcacaagcgg agaacagcca tattgtgtct attttgctta
     1441 accgcaatta cgggcaacat tcagcgatta tggcgggatt cagtcacgtt actggcgact
     1501 taattattac ccttgatgcc gatctccaga atccgccaga agaaatcccc cgcctggtgg
     1561 caaaagccga tgaaggttac gacgtggttg ggactgtacg ccagaaccgc caggacagct
     1621 ggtttcgtaa aaccgcttcg aagatgatta accggcttat tcagcgcacc actggcaaag
     1681 cgatgggtga ctacggttgt atgctgcgcg cctatcgccg tcatattgtc gatgcgatgt
     1741 tgcactgcca tgaacgcagc acctttatcc cgattctggc gaatatcttc gcccgccgtg
     1801 caattgaaat tccagtacat catgccgagc gtgagtttgg tgaatccaaa tacagtttta
     1861 tgcgcctgat taatttgatg tacgacctgg tgacctgcct taccacaacg ccgctacgta
     1921 tgctgagtct gctcggcagc attattgcga ttggaggttt tagcattgcg gtgctgctgg
     1981 tgattttacg cctgaccttc ggaccacaat gggcggcaga aggcgtcttt atgctatttg
     2041 ccgtgctgtt tacttttatt ggcgctcagt ttatcggcat gggattactc ggtgaatata
     2101 tcggcaggat ctacaccgat gtccgcgccc gcccccgcta ttttgttcag caagttatcc
     2161 gtccatccag caaggaaaat gaataatgaa aaccgtcgtt tttgcctacc acgatatggg
     2221 atgcctcggt attgaagctc tgctggctgc cggttacgaa attagcgcca tttttaccca
     2281 taccgataat cccggtgaaa aagcctttta tggttcggtg gctcatctgg cggcggaaag
     2341 aggcattccg gtttatgcgc cggataacgt taatcatccg ctgtgggtgg aacgcattgc
     2401 ccaactgtca ccagaggtga ttttctcttt ttattatcgc catcttattt gcgacgagat
     2461 tttgcagctc gctcccgcag gtgcatttaa tctgcacggt tcgctgttac caaaatatcg
     2521 tggtcgcgcg ccgctgaact gggtgctggt gaacggtgaa acggaaacgg gcgttacatt
     2581 gcaccgaatg gtaaaacgtg ccgatgctgg ggccattgtg gcgcaactgc gcgttgccat
     2641 tgcgccagac gatatcgcta ttacgctgca tcataaattg tgccatgccg cgcgccagct
     2701 actggaacag acattacccg ccattaaaca cggtaatatt ctggaaatcg cccagcgcga
     2761 aaacgaagcc acctgttttg gtcgcagaac gccggatgac agtttccttg aatggcataa
     2821 accggcatcc gtactgcaca acatggtacg tgccgttgcc gatccgtggc cgggtgcctt
     2881 cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc ctcatgccag
     2941 caaagcacag ccggggagcg tgatttctat tgcaccgctg ttgattgcct gtggcgatgg
     3001 cgcgctggaa atcgtcaccg gacaggcggg cgacggcatt actatgcagg gctcgcaatt
     3061 agcgcagacg ctgggcctgg tgcaaggttc acgcttgaat agccagcctg cctgcaccgc
     3121 ccgacgccgt acccgggtac tcatcctcgg ggtgaatggc tttattggca accatctgac
     3181 agaacgcctg ctgcgcgaag atcattatga agtttacggt ctggatattg gcagcgatgc
     3241 gataagccgt tttctgaatc atccgcattt tcactttgtt gaaggcgata tcagtattca
     3301 ttccgaatgg attgagtatc atgtcaaaaa atgtgatgtc gtcttgccgc tggtggcgat
     3361 agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg attttgaaga
     3421 gaatctgcgc attatccgct actgcgtgaa gtaccgtaag cgaatcatct tcccgtcaac
     3481 ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggaccatt ctaatttaat
     3541 cgtcggcccg gtgaataaac cacgctggat ttattcggta tcaaaacaat tacttgatcg
     3601 ggtgatctgg gcctatggcg aaaaagaggg tttacagttc accctcttcc gcccgtttaa
     3661 ctggatggga ccacgactgg ataaccttaa tgcggcacga atcggcagct cccgcgctat
     3721 tacgcaactc attctcaatc tggtagaagg ttcaccgatt aagctgattg atggcggaaa
     3781 acaaaaacgc tgctttactg atattcgcga tggtatcgag gcgttatacc gcattatcga
     3841 aaatgcggga aatcgctgcg acggtgaaat tatcaacatt ggcaatcctg agaacgaagc
     3901 gagcattgag gaactgggcg agatgctgct ggcgagcttc gaaaaacatc cgctgcgcca
     3961 tcatttccca ccgtttgcgg gctttcgcgt tgtcgaaagt agcagctact acggcaaagg
     4021 atatcaggac gtagagcatc gtaaaccgag catccgcaat gcccaccgct gcctggactg
     4081 ggagccgaaa attgatatgc aggaaaccat cgacgaaacg ctggatttct tcctgcgcac
     4141 cgttgatctt acggataaac catcatgacc aaagtaggct tacgcattga tgtcgatacc
     4201 tttcgtggca cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa gcataatatt
     4261 caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct ctggcgactg
     4321 gtgaagccac agtttttgtg gaagatgctg cgctcaaacg cggcatcgct ttatggctgg
     4381 gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc caatgccgat
     4441 atcattcgtg aagcggctaa acatcacgaa gtcggcctgc acgcctggga tcaccatgcc
     4501 tggcaagccc gtagcggtaa ctgggatcgg caaacaatga tcgacgatat tgcacgcggt
     4561 cttcgcactc tggaagagat tatcggtcaa ccggtaacct gttctgccgc tgcgggctgg
     4621 cgtgccgacc agaaggtgat cgaagcaaaa gaagcgttcc atttgcgcta caacagcgat
     4681 tgtcgtgggg ccatgccgtt ccgtccattg ctcgaatcag gaaaccctgg cactgcgcaa
     4741 attccggtga ccttacccac ctgggatgaa gtgattggtc gggatgtgaa agcagaagat
     4801 tttaacggtt ggttactcaa ccgcatcctg cgagataaag gcacgccggt ttataccatt
     4861 catgcagaag ttgaaggctg cgcttatcag cataattttg tggatctcct caaacgcgca
     4921 gctcaggaag gcgtgacatt ttgctcttta agcgaactgt tatcagagac gttgccgctc
     4981 ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg ccaacaaatt
     5041 gcgggtagtc gctgatgaaa tcgttacgtt accttatcgg cctcttcgcg tttattgcct
     5101 gctattacct gttaccgatc agcacgcgtc tgctctggca acccgatgaa acgcgttatg
     5161 cggaaatcag tcgagaaatg ctggcatccg gcgactggat tgtgccccat ctgttagggc
     5221 tacgttattt cgaaaaaccc attgccggat actggattaa cagcattggg caatggctat
     5281 ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg ttaactgccg
     5341 cgctggtgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct ctactcgcca
     5401 cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc gtgctcgatc
     5461 cgtttattgc attctggctg gtggcgggaa tgtgcagctt ctggctggca atgcaggcac
     5521 agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc ggcatggggg
     5581 tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg ccatgggtag
     5641 cgacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtt atcagttgcg
     5701 tactgacggt tctcccttgg ggactggcga tagcgcagcg ggagcctaac ttctggcact
     5761 attttttctg ggttgagcat attcaacgct ttgcactgga tgatgcccaa catagagctc
     5821 cgttctggta ctacgtgccg gtcatcattg ccggtagcct gccgtggctg ggattactcc
     5881 ccggtgcact gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc tatttgttga
     5941 gctggacgat aatgccgctg ctgtttttct ccgtcgctaa aggtaaattg cccacctata
     6001 ctctttcctg ctttgcatct ctggcaatgc tgatggcgca ttacgctttg ctggcagcaa
     6061 aaaataatcc tctggcgctg cggattaatg gctggattaa catcgctttt ggcgtcactg
     6121 gcattattgc cacatttgtg gtctccccgt ggggaccaat gaacacgccg gtgtggcaaa
     6181 ccttcgagag ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg gcatttttcg
     6241 gctggtacac cttaacaaac gtcgaaaaga cctggccttt tgccgcgctt tgcccgctgg
     6301 ggctggcgtt gctggtagga ttttcaattc ctgacagagt tatggaagga aaacatccgc
     6361 aattttttgt cgagatgaca caagaatcac ttcagccaag ccgctatatt cttactgata
     6421 gcgtcggtgt tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc atcatgtatc
     6481 gccagacagg tgagttgaaa tacggcctta attatccgga tgcgaaaggg agatttgtca
     6541 gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt attactctcg
     6601 tgctttcggt tgaccgcgat gaagatatca acagtctcgc cattccgccc gcagatgcca
     6661 tcgatcgtca ggagcgtctg gtgctgattc agtatcgtcc caaatgatct ggctaacatt
     6721 agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg caacctgctt
     6781 tgtggcgata aataaacggc gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg
     6841 tcttggtctt gccatggtgc tctggctgct ggtcttgcag aacgtaccgg taggcattgc
     6901 ctacccgatg ttaagtctga attttgtctg ggtgacgctg gctgcagtaa aactgtggca
     6961 cgaaccggta tcgccgcgtc actggtgtgg ggtggcgttc attattggcg gcattgtgat
     7021 cctcgggagt acggtgtaat gggcctgatg tggggattat tcagcgtcat tattgcctca
     7081 gttgcgcaat taagcctggg ttttgcggcg agtcatctgc cgccgatgac gcacttatgg
     7141 gattttatcg cggctctgtt agcttttggc ctggatgccc ggatactgtt actcgggctg
     7201 ctgggttatc tgctttccgt tttttgttgg tataaaacgc ttcataaact tgcgctaagc
     7261 aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc aatggtactt
     7321 cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg tattatgagc
     7381 gggttgatgc tgattttcct gcccacgaca aaacaacgtt actgagtttt ccctgccact
     7441 ttacactgcc gttcccactc atcggggcta taactgcttg ccgagaggac tttcaccgta
     7501 tgcagctttt cacgattaat acaataaagc gcattctgca aaggcgagag taaatccccc
     7561 actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg
     7621 cagagcataa gtacatgtct gttatcttgt ttattgcagc acgatttttt gaccagccat
     7681 tccattgcat tatcctgttt gctaagagtt ttcacatcaa tttacagatt aacagataaa
     7741 ctctttatca acggcaatgg aatggcagaa atgagaagtg aaacggaggg ttaattattg
     7801 ctgacgttgc acccactctt ttagcgcctg acgtgaaatt ttaataccgc cgtttttcag
     7861 ctccggcggt aaagtcagcc agcgcaccgg ttgttgaaaa cgagccagct tatctttcac
     7921 ccattcgcca agatcaacgg tttgctggtc atactccacc accgctaccg gccgatgacc
     7981 aaactccttg tcggcaacgg gaacgataaa cacctgcaaa accgcaggat gtgcagcaat
     8041 aacacgctcg acttcttccg gctgaatacc ctcaccgcca ctgaagaaca gattgtctaa
     8101 acgtccgaca atggtcagct tgccattatg catctcaccg cgatcgcgcg tagcgtacca
     8161 gccttcgtca ttaaccagtg aaaccagttg cccgttacgc cagtaacctt ctgccatact
     8221 ggcagcccgc agccacactt cattattaac gattttcact tcccgacccg gcagcggcga
     8281 accaacgtct gccaggccgt cggcttcttt cgcacatacc gtggaggcaa actcggtcag
     8341 accatagccg caaaagcaac gaatcccctg ctcgcgcgcc tgttccgtca actcgaccgg
     8401 gatagccgcg ccgccaagta acaccgcttt cagggaaacg gaactgcggt taaccagcaa
     8461 acgccagagt tgtgttggca ccagtgaagc gtgagtacag cctgccagca tttgctccaa
     8521 tggctgttta tcacgcaccg tcatccgcgc accagcgtat aaccagcgcc acataattcc
     8581 ctgaccggag acgtgaaaca gcggtaaaga gagcaaccag tcatcgtgat cgccaaacgg
     8641 aatcagcgat aacacacctt gcgcactggc aagatgggcc tgataagtat ggacagcggc
     8701 tttcggcaaa ccggtagaac ccgaggtcaa cgtcattgaa cacagacgcg tcggctgcca
     8761 cgtagcggca tgtgcgcctt caacccgctg aatgtgcagc gacgttaatg ccggaaacgt
     8821 gttttcccca tccggcacca gcgcaaattg cagcgtcaga ttgggcagca attcttcaag
     8881 caacggttgc ggcagctgag ggttcacggg caacacccgc gccccgcatt gcagtaacgc
     8941 cagccaggcg agcagcgttt gcggcgtatt ccacgcccgc aacatcacgc cgctgccctc
     9001 aaccacaccc tgcgccgcaa agcccgtagc taactcatcg acgcgagcac aaagctcgcg
     9061 ccagttgagt tgctcgtcat taagacgtaa ggcgatggct tctccccgca cttgccgcca
     9121 gtgacgccac ggccagtcag agaagatcat aacaaccgct ccagtgcatc aacttccacg
     9181 acaggcagcg tgctacctgg ccagcgacgt acctgctgcg cctgcatcag atccagcgtg
     9241 tccagccctg gaatggtgtc cggcgttaac caggcggcaa tccgcgccag ttgcgttaag
     9301 cctaagctcg actcaataga agaactgatc accgccgtca gccccaacgc gtgcgccgcc
     9361 tgtacctgct cgcgtacttt ttccagactg cccgtgagcg tgggtttgat aactaccgcg
     9421 cgcacgccct cttcagccac aaaggcaaaa tccggctcgc gcaggctttc atcccaggca
     9481 atggcaatgc cggtttcacg ggcaaacgct cgcgaatcat cgcgggtttt gcacggctct
     9541 tcgagaaacg cgatgcggtg gcgataatcc gggttaacat atttggcaaa ctgctgacct
     9601 ttcagcggtg tccaggcgcg atttgcgtca agacgcaaat gcagatccgg aattgcctcc
     9661 agcaacagat taaccaccat gccgtcgcgc acggcttcgt acaatcccac tttgaccttc
     9721 gccactttct cgcctggcat atcagcaagt ttgaggatca gatcgtcagg atcgccatta
     9781 cacagtggtg ccgcacggta attggccgcc tgcgggagcg tatctgccag ctccgccaat
     9841 gcacagctta cgccaaaggc aacggaaggc atctgcggta gctcgcaatc gcctgccagc
     9901 cagttattta cccaggcaag cagcacgctt tgcgcctctt cccaggtttc ctggctgaag
     9961 cccggcagtg gggagatctc cccccaccct tcgcgctcgc cctcacgcag gcaaacgtac
    10021 agcccgtcgc gggtttttaa ccgcctgtcg cgcagaacca cccccgcgtc catggggatc
    10081 tgccagcggt atacctgcgc gctacgcatt acggattccg tttaaatttg ctgaaatcag
    10141 gctgacgttt ctggttgaaa gcgttgcgac cttcctgacc ttcttccgtc atgtagaaca
    10201 gcatggtggc gttgcccgcc agctcctgca gccccgcctg cccgtcacag tcggcgttca
    10261 gtgcagcttt caggcagcgc agcgccatcg gactgttttg cagcatttcg cggcaccagc
    10321 gaacggtctc tttttccaga tccgccagcg gtaccacggt gttgacaagg cccatatcca
    10381 gcgcctgttt cgcgtcgtac tgacggcaca ggaaccagat ttcccgcgct tttttctgtc
    10441 cgacgatgcg agccatgtag gaagcgcccc agccgccgtc gaaggaaccg actttcgggc
    10501 cagtctgacc gaagatggca ttatctgccg cgatagtcag gtcgcacatc atgtgcagaa
    10561 cgtgaccgcc gccgatggag tagccagcca ccatcgcgac aaccggtttc ggacaggtac
    10621 ggatctgacg ctggaagtcc agcacgttca ggtgatgtac gccggaatca tctttatagc
    10681 cgccgtaatc accacgcact ttctggtcac cgccggagca gaacgcttta tcacctgcgc
    10741 cagtcagaat gatcacgccg atattgtcgt cataacgcgc atctgccagt gcctggatca
    10801 tctcttttac cgtcagagga cggaaggcat tgcgcacctg cggacgatta atggtgattt
    10861 ttgcgatacc gtcggtggat ttttcataac gaatgtcctc gaaaccttcg gagcagtcgt
    10921 gccattcaac cggtgcgtaa agcattgctt catcaggata aatcatattg tgtcctttag
    10981 tcagaaacgc aagatctgcg ccagacttgc gattacgcca gcgggatttt cccgatgcgc
    11041 gttatgtccg gcgcgaggaa tgacatggca gtcggcagcc agttccgccg ccagggcgcg
    11101 gaatttgctg tcacgttcac cacataaata ataaaacgca aatgtgcggg cgctaaggtt
    11161 agcacgtaaa tcaggctgga cggcgagaga agtcgcctcc agcatggcgg caagggttgc
    11221 gccattattg ttgctgcgca gcgccaccag ctcccggcgt tgatcgtcat tgagcgaggc
    11281 aaaaacaggc tgttgatacc agtcggcaaa taccgccgtt aacggttctg tgcgaaaacg
    11341 ctgcgcccat tggcgatcgg aacgctgacg ttccgcacgt tgttcagcat tttgcagccc
    11401 cggatgcccg ccttcgacaa taaccccaca aagccccgcc agcccctggc aagccgccat
    11461 catcgccacc cgtccgccaa gcgagtaccc caccagccag aagtcaagga tgttgtaact
    11521 aaccaaggtt ttacgcagta agtcggtgac atcatcaaat ccatcgacgc taatcgccgc
    11581 cgaaccacca tgacctggga gatcaacata caaccgtgag tagtcggcaa acgcctcgcc
    11641 cacttcttgc cattcgtggc aatcgccgga aaaaccgtgc aaaaacacca gccagggtaa
    11701 acctggtttt ccgtgttttg cctgcgcgtg caggatcata aatggcttac ctgcgccaga
    11761 agttgctgga gcgtttgcgc accatcggtg tcgttaacca ccatttcaat caccgtggtg
    11821 gttggcgtgc gccaggcgtc ggcaaatgcc gtttcaagtt cctgccagtt ttgcggacga
    11881 tgatatttca gctcgaacat cgcggcggcg tgctcaaaat ggacgttttg cggcatcaga
    11941 tagaaacgct cacgctcgct ttgcggcgtt ggcaacagcg agaaaatttg cccgccgttg
    12001 ttgttcacca caattaatac cagcggcgca gaaacctgac gcaataacgc cagcgcgttg
    12061 agatcgtaaa gtgcggagag atcgcccaca atcgccagcg tcggtttgcc gcttgcccgc
    12121 tgaacgcctg cggcggtcga aagcagtccg tcgataccgc tggcccctcg gttgctgtac
    12181 accgggtaac ctgccggaag ttgcgaaagc gcatcaatca gacgtaccac caggctgtta
    12241 ccaacaaaca attgcccctg ttcaggcaga tagtcgcaga tgcgatgcgc cagttgcgct
    12301 tcgccaaacg catcacggcg ggcaataacc gcctgcattg cctgttccgc caggcgcggg
    12361 atttcaacgc accagggctg gcgtttttct gccggatgca gctccagcca gtcggcaata
    12421 ttggcaatta agcgacgtcc gcgatggtgt gccggatcaa gtcgcccttc aatgtcatca
    12481 acaatccagt actcttctgg ttcacagctt gcctgccatt gcaggagccg tttccccgtc
    12541 aggctgcttc ccagttgcac cacaatttgc gcctgctgca gctcgctggt cgctttggca
    12601 ttgcctaacc aaagatcggc acacgccagc ggctgcccgg tttgtgacag cacatcgcca
    12661 atcagcggcc agccaagagt ttgcgcccac agggcaactt ttttgccctc ttccgcactc
    12721 atgcgcccgg caaccaccac gccgcgcttt tgtcgccaga agaaccagtc gcgctgtttt
    12781 tcactttcca gacgaggcgc ttcacgcagc cacggtttgt cgtcctgcca ccagtcaccc
    12841 agacgctgtt gccagctaag cccggtatcg tccatttcgc catacagcgg ttcagcaaac
    12901 gggcagttga tatggactcc ccccgcatga agcgtaccga gagcgtggtc gatggtagaa
    12961 accagccaac gtgcggggat atcttgggtc gggcgcggca atgaaatact gtgcgtgggg
    13021 tgagaggcga acattcccgg ctggcgaatt gcctgattcg cgccgcagtc aattagctcc
    13081 ggcgggcgat cggcggttaa gagaatcagt ttttctccgg ttaacccggc ttcaatcagt
    13141 gccggataga gatttgccac cgccgtgccg gaggtcacaa tcaccgccac cggctgcttg
    13201 ctgactttcg ccagccccag cgccagatgc cccaacccac gctcatcgaa atgggtgtgg
    13261 tgaatgaatg cggaattctc cgccgccgct aacgttaacg gtgtagaacg cgagcctggg
    13321 gcgatacata tgtgtctgac gccgtgacgc gttaatgctt ccagaatgac cgccgcccag
    13381 cgtcggttaa atgcgcttac tgacatgagt ttgtccggta tcaatattgc ggctaagtat
    13441 aaggagctac aaaaatagat tattgatatg aatcggtaat gatgcgactc attactattc
    13501 catctgtaat aaagtacgca gccctgccgc tttgttgtcg atttcctgcc actcttgctc
    13561 ggggtcggaa ccacggacaa tgcccgcgcc agcatataat cgcacgacat tgccgctaat
    13621 ttttgctgag cgcagggaaa cgcagaattc gctttgttgt aatgagagat agcccgcaga
    13681 accggcgtac cattctcggg tgaacggttc gtgacgggcg ataaactgtc gcgccagatc
    13741 gcgcggtaag ccagcaactg ctgccgttgg ctgcaactga tgtaaacaga tcacatcatc
    13801 cgctttgttg agtgaagtcc agatacagcg gcgaagatgc tgcactttac gcagacgcag
    13861 tacctgcggc ggtaaaacat ccagcgtctg ggtatcggcc tgtaatcgtt ggcagatatc
    13921 taccaccacc agcatgttct cgcgctggtt tttatcatcc gccatcagcc actctcctaa
    13981 ctgctgcgcc tgcttatcat caggatgatt tgctactgtt cccgccagcg cttcagtacg
    14041 cagcgctttg tcacgccgcc gccataaccg ttccggtgaa gagccaagaa aagcattttc
    14101 gccatcaaag gccatgtaaa aatggtagca attcagattc agtcgacgac tggcagccat
    14161 catcgccgcc gcgttgaccg gacttgcgaa atgcaggtca gttgcccgag cgagcaccac
    14221 tttgtcgagc tcaccttcgg cgatggtttt cgttgccagt tcgattaatt gcgtccagcc
    14281 cgttttgtcc ggccagtgtt gttctcgcgt ggtggttaaa tgtaacccag gcaagggctt
    14341 gatactcacc agtgtggcga taaattcttt tgcctgaatc gcatcgtgct gaagggagct
    14401 ttcgctgaat agcgtcagcc gcagcgtggc tttaccgcca cagcgtcgcc attccaggcg
    14461 gggtaaaagt aaattgccct gcgacgggtc aaaagcattc agcccccaaa tgcgtaagtc
    14521 ggcgtgttcc gggtgctggc gaagaaaacg ttgtgcctgg tccaacgacg taaaacgggt
    14581 aatcgcgccc aggacgacag cttcttcatc accattacgt tgttgccagt agaattgcgg
    14641 gtacgtttgc tgactggcca gccagctcaa ggcatcaaaa gcgtctttga gagggaaagg
    14701 aatatcgata acccgaatgc cgggtgtcgc cggaatctct tgcgacaaat ggcgcagtag
    14761 attttccagc gccgtagtaa gtgattgcac gcggacctct ccctgttaaa agcctcacat
    14821 tatacggggt actacaaaaa aaatgcagta ccccggtgta gggaggtttt aacggcgtgc
    14881 cagcaacagt cctagtacca gcccaacggc cgcgcccaca ccaattcctt gccagggttt
    14941 ttcgtggacg tagtcatcag cacgataaac tgcctgcttc gcccgatagt aataactgtc
    15001 tgaagcctgg ctaacccgtt ttttcacatc atccagcgct ttttctgcac gcgctttcag
    15061 ctcaacatat ttctgatcgg cgggatcgcc agaggagcgg agcacctctt ccagtgtttc
    15121 actaagcagc gtcaggtcgt catcgatacg tgtatcacca aactgattag acattctcgt
    15181 tctccatttg cgtaaaacct gtcactaact atagacaagg gttgttgatt acgcctgaat
    15241 tacctcgcgt gccatcccaa tgtgtgggat accatcttct tcatagacct ccgtcacagg
    15301 gataaaaccg aaactctggt agaagttttg cagatgcgcc tgcgccccga ggtaaacagg
    15361 cttatcaggc cagtgatgcg tacatgtttc gagtgtttta ctcatcagct gctgccccac
    15421 tttttcgcca cgcaacgctt cactgacaat cacccgacct ataacgaccg gctcaagatc
    15481 atcatcactt ttcagaatcc tcgcatacgc caccagttca tcatttttcc aaccgagaat
    15541 atggcgatta tcccccgtca aatcatcacc atcgatatcc tgataagggc aattctgctc
    15601 gacaacaaac accgcgcaac gcagttgtaa taaggcataa agctgtgaaa ccgataattc
    15661 agagtgatgc agatcttgcc attcaatcat aatgctctcc ttttagatgt atgccttata
    15721 ctagccggaa tattttttga aacgtgatga actcatggaa ttaatttttt taggtacttc
    15781 agccggtgtg ccaacccgca cgcgcaatgt cacggcaata ttgcttaacc tgcaacatcc
    15841 gacccagagc ggactttggt tgtttgactg cggtgaaggc acccagcatc agctactgca
    15901 taccgccttt aaccctggaa aactggacaa gatttttatc agtcaccttc atggcgatca
    15961 tctttttggt ttacccggct tgctgtgcag tcgttctatg tcaggcatta tccaaccctt
    16021 aacaatttat ggtccgcaag gtatccgtga atttgtggaa accgcgctgc ggattagcgg
    16081 ctcatggacc gattatccgc tggaaattgt cgaaattggc gctggcgaaa ttctcgatga
    16141 tggcctgcgc aaagtaaccg cttatccgct ggaacaccca ctggaatgtt atggctatcg
    16201 tattgaagaa catgataaac cgggtgcatt aaatgcccag gcattaaaag ctgctggcgt
    16261 gccgcctggc ccactgtttc aggaattaaa agcgggcaaa acaatcacgc tggaagatgg
    16321 aaggcagatt aacggcgcag attacttagc tgctccagtg ccaggtaaag cgctcgctat
    16381 tttcggcgat accggcccct gcgatgccgc acttgacctg gctaaaggtg tcgatgtcat
    16441 ggtgcacgaa gcgacgctgg atataaccat ggaagccaaa gccaatagtc gcggccatag
    16501 ctctacacgc caggctgcga cactagcccg tgaggctgga gtcggtaagc taatcattac
    16561 ccacgtcagc tcgcgctatg atgacaaagg ttgtcagcac ctgttacgtg aatgcaggtc
    16621 aattttcccg gcgactgaac tagcgaatga tttcaccgtg tttaacgttt aactctgctt
    16681 attattaaaa acagggcgaa acttgccctg ttatcgcaac ccgcgctaca tatatccacc
    16741 cacacgggca tattgagcga tttttgatat ttattcatct ccttacgcac tatattcaga
    16801 gcaataagtt gcctcttttt atcttcattc agcacaacct ttgcaactaa aaggagccca
    16861 tgatggttac agttgtcagc aattattgtc aattatctca aacccaactc agtcagacat
    16921 ttgcagaaaa atttactgtg accgaggaat tactgcagtc tttaaaaaaa cagcgttatc
    16981 cggagatgaa gaaagtattg agttactgca taatattgcg ttaggttatg atgaatttgg
    17041 aaaaaaagct gaagatattc tttaccatat tgttagaaac ccaacaaatg agaccctatc
    17101 gattatccga cttattaaaa atgcctgttt aaaattatat aatttggcac atactgcaac
    17161 caactcccac cttaaaccaa ctggtccgga taattcagat gttctcctgt tcaaaaagct
    17221 attctcccct tcgaaattaa tgacaattat cggtgatgaa attcctctta tatctgaaaa
    17281 acagtcgctt tcaaaggtgc ttttaaatga tgagaataat gaactgagtg atggtacaaa
    17341 cttctgggat aaaaatcgtc aattaaccac agatgaaata gcttgctatc ttcagaagat
    17401 cgccgctaat gcaaaaaata ctcaagtcaa ttatcctact ggtctctacg taccctactc
    17461 caccagaact cacctggaag acgctctcaa tgaaaatatt aagagcgatc catcatggcc
    17521 gaatgaagtc ctgttattcc ccataaatac tggcggacac tggatattag tttcgctaca
    17581 gaaaatagta aataaaaaaa ataa
//
LOCUS       DAZKHC020000084        17569 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZKHC020000084 DAZKHC020000000
VERSION     DAZKHC020000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.084"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..216
                     /locus_tag="ACHOSM_RS17375"
                     /old_locus_tag="pgaptmp_003475"
     CDS             <1..216
                     /locus_tag="ACHOSM_RS17375"
                     /old_locus_tag="pgaptmp_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559915.1"
                     /translation="TDAFLAEVRAQGVDAAIEAAKNLVAQEYEYKDFKAAQSDCCMHP
                     GSDLVGKVEMTEWLVDFAAQLRKGGNQ"
     gene            213..446
                     /locus_tag="ACHOSM_RS17380"
                     /old_locus_tag="pgaptmp_003476"
                     /pseudo
     CDS             213..446
                     /locus_tag="ACHOSM_RS17380"
                     /old_locus_tag="pgaptmp_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289896.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            483..701
                     /locus_tag="ACHOSM_RS17385"
                     /old_locus_tag="pgaptmp_003477"
     CDS             483..701
                     /locus_tag="ACHOSM_RS17385"
                     /old_locus_tag="pgaptmp_003477"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559916.1"
                     /translation="MRVELEAEKQRAKDLFMENARLKSGIAGLIHLGIRYADVDVMKI
                     AGDAQLSTPCTDSIINSIATGIRINGGE"
     gene            1047..1235
                     /locus_tag="ACHOSM_RS17390"
                     /old_locus_tag="pgaptmp_003478"
     CDS             1047..1235
                     /locus_tag="ACHOSM_RS17390"
                     /old_locus_tag="pgaptmp_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559917.1"
                     /translation="MKNRKAKILLVRRNAPGVWQWVRLSNRRMGLMKYYGMMDCGFCK
                     KPSAAQNRWKNHLRTKGE"
     gene            1237..1415
                     /locus_tag="ACHOSM_RS17395"
                     /old_locus_tag="pgaptmp_003479"
                     /pseudo
     CDS             1237..1415
                     /locus_tag="ACHOSM_RS17395"
                     /old_locus_tag="pgaptmp_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1749..2093
                     /locus_tag="ACHOSM_RS17400"
                     /old_locus_tag="pgaptmp_003480"
     CDS             1749..2093
                     /locus_tag="ACHOSM_RS17400"
                     /old_locus_tag="pgaptmp_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559918.1"
                     /translation="MTTITREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALASLAAEPAGKLHEYKPVGHQRLVDELTMLVKQLTWQLRKTKPDCKLPGKAMDYLK
                     RNGLISAEDVLR"
     gene            2176..2409
                     /locus_tag="ACHOSM_RS17405"
                     /old_locus_tag="pgaptmp_003481"
     CDS             2176..2409
                     /locus_tag="ACHOSM_RS17405"
                     /old_locus_tag="pgaptmp_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559919.1"
                     /translation="MGKITFVVEFEDGKEPPVSANLDVAGGRLVSVLFGDYRDDFFQP
                     EEVDVVREALNELSVDNDDAHAEIIKKMELLTH"
     gene            2588..2881
                     /locus_tag="ACHOSM_RS17410"
                     /old_locus_tag="pgaptmp_003482"
     CDS             2588..2881
                     /locus_tag="ACHOSM_RS17410"
                     /old_locus_tag="pgaptmp_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559920.1"
                     /translation="MWRGNSHGKSQMILTEYQLDHKTNKSRSVYLLRHNSRVRNTVLE
                     QNLTVEMDNYGGFKPTISLDDFPRGLSEREAMLKLAEWLQRLSIAIEDNWSEP"
     gene            2888..3262
                     /locus_tag="ACHOSM_RS17415"
                     /old_locus_tag="pgaptmp_003483"
     CDS             2888..3262
                     /locus_tag="ACHOSM_RS17415"
                     /old_locus_tag="pgaptmp_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559921.1"
                     /translation="MMTLKHFLDRPLWAAAAGYDFNYMDCMSYTANAYDHSFSLLFNS
                     LRILPQTEVGELHLWLLGFIAAGVGIAVWPFIFWLVAVVVWFKCKTYRKKYFLGDGMT
                     DIAKMNIEKWTKECEKKWRKKK"
     gene            3244..3924
                     /locus_tag="ACHOSM_RS17420"
                     /old_locus_tag="pgaptmp_003484"
     CDS             3244..3924
                     /locus_tag="ACHOSM_RS17420"
                     /old_locus_tag="pgaptmp_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559922.1"
                     /translation="MAQKEMTTLTTAYLQQLVFFAGEATCHPDANYLLEFERLASPGI
                     VLELAQQVLALRQKEQHESNTCRLNFEQWLEQQRGKIDVDCGCVSTETFMHWLRVAYE
                     AGNYPDIPDSSVPAPGKGVTGERIRIKPHVYRELVNRLHDTAIKCAGTRQLRERISRV
                     LGDVITPDHHKQAEKSDLERCHLEAALNIKPGHTLGIIDALLVHKMARALLPLVAEKH
                     EADHANES"
     gene            4197..4475
                     /locus_tag="ACHOSM_RS17425"
                     /old_locus_tag="pgaptmp_003485"
     CDS             4197..4475
                     /locus_tag="ACHOSM_RS17425"
                     /old_locus_tag="pgaptmp_003485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559923.1"
                     /translation="MFRPRPIPPKITNGYQPNTPEIQGSEGLKPPPAPRIPPVARNVS
                     LRRELILALSPVLAARQDWVSNDDLAEKITSLVDKILQIGNEITLIHW"
     gene            4597..4986
                     /locus_tag="ACHOSM_RS17430"
                     /old_locus_tag="pgaptmp_003486"
     CDS             4597..4986
                     /locus_tag="ACHOSM_RS17430"
                     /old_locus_tag="pgaptmp_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU4559924.1"
                     /translation="MGFPSPAADYVESRISLDQQLIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCAMEGEYRIKRYRKYPRQHLEDLSTGKKEALPVDDDGYT
                     GSNAVFGVITHVINDARSGEFDDCPVI"
     gene            5059..5280
                     /locus_tag="ACHOSM_RS17435"
                     /old_locus_tag="pgaptmp_003487"
     CDS             5059..5280
                     /locus_tag="ACHOSM_RS17435"
                     /old_locus_tag="pgaptmp_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGU4559925.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            5280..5660
                     /locus_tag="ACHOSM_RS17440"
                     /old_locus_tag="pgaptmp_003488"
     CDS             5280..5660
                     /locus_tag="ACHOSM_RS17440"
                     /old_locus_tag="pgaptmp_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HGU4559926.1"
                     /translation="MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            5665..5844
                     /locus_tag="ACHOSM_RS17445"
                     /old_locus_tag="pgaptmp_003489"
     CDS             5665..5844
                     /locus_tag="ACHOSM_RS17445"
                     /old_locus_tag="pgaptmp_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HGU4559927.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLVGKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            5872..6915
                     /locus_tag="ACHOSM_RS17450"
                     /old_locus_tag="pgaptmp_003490"
     CDS             5872..6915
                     /locus_tag="ACHOSM_RS17450"
                     /old_locus_tag="pgaptmp_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4559928.1"
                     /translation="MKAVITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMDDGLVVSEQTMATNEALQPFFNSDELFRRIGGIDSLVAWLRRKEGQCQAADRSWC
                     DNHIVYAERDNSAVLLCWHHDNHYRMRGFNELKETLHNNRVNWILDVARQEMGLSDGH
                     DLSIQELCWWAFMRNMMHLMPEEVCRISINKMKAATQDSGPLKEADIRPYDDRATAYV
                     QMMEERAAPMRAKVCPVDVDSDPGMAHFKIPKLQSLKLPEYMDFVASRPCCGCGAAGA
                     GAHITPYIVRHSRLCAHDIYAIPLCQSCQRDIERDRDNWEKTHGRLAMHQRLFFDYAL
                     GVGAITSHSSSVR"
     gene            7004..7456
                     /locus_tag="ACHOSM_RS17455"
                     /old_locus_tag="pgaptmp_003491"
     CDS             7004..7456
                     /locus_tag="ACHOSM_RS17455"
                     /old_locus_tag="pgaptmp_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Late promoter-activating protein"
                     /protein_id="HGU4559929.1"
                     /translation="MLLNWQGRHFMEINHSRITSYEIADYMIRTKSLLSAKELAAILE
                     KEYPHLDVDKRDVYLRLKAIAVSKYSSVLIDDSTRPRRFQIHSLNPEFFRRSRAPRRF
                     DEKLQNELYMTQDEKERREHQPWVMARQLFNKVARQHRHYGNATSARI"
     gene            7542..8735
                     /locus_tag="ACHOSM_RS17460"
                     /old_locus_tag="pgaptmp_003492"
     CDS             7542..8735
                     /locus_tag="ACHOSM_RS17460"
                     /old_locus_tag="pgaptmp_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4559930.1"
                     /translation="MTWDDHKKNFARLARDGGYTIAQYAAEFNLNPNTARRYLRAFKE
                     DTRTADSRKPNKPVRKPLKSMIIDHSNDQHAGDHIAAEIAEKQRVNAVVSAAVENAKR
                     QNKRINDRSDDHDVITRAHRTLRDRLERDTLDDDGERFEFEAGDYLIDNVEARKAARA
                     MLRRSGADVLETTILEKSLSHLLMLENARDTCIRLVQEMRDQQKDDDEGTPPEYRIAS
                     MLNSCSAQISSLINTIYSIRNNYRKESREAEKHALSMGQAGIVKLAYERKRENNWSVL
                     EAAEFIEAHGGKVPPLMLEQIKADLRAPKTNTDDEERQTAVGGPSLEDLDKVARERAA
                     NRRADAALWIEQRREEIADIVDTGGYGDVDTEGVSNDPWLEQDLDEDEEEDEEVTRKL
                     YGDDD"
     gene            8735..10228
                     /locus_tag="ACHOSM_RS17465"
                     /old_locus_tag="pgaptmp_003493"
     CDS             8735..10228
                     /locus_tag="ACHOSM_RS17465"
                     /old_locus_tag="pgaptmp_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4559931.1"
                     /translation="MARSCVTDPRWRELGALYRYDWIAAADVLFGKTPTWQQDLIIES
                     VQEQGSKTSVSSGHGTGKSDMTSIMIMLFIIMYPGARAIIVANKIQQVMTGIFKYIKI
                     NWATATSRFPWLADYFVLTETAFYEVTGKGVWTVVPKGFRLGSEEALAGEHADHLLYI
                     IDEASGFSDRAFGIITGALTGQDNRILLLSQPTRPSGYFYDTHHKLAKRPGNPDGVYT
                     AITLNSEESPLVTPAFIKMKLAEYGGRDNPMYMIKVRGLFPKSQDGFLLGRDEVERAT
                     RRKVKIAKGWGWLACVDVAGGTGRDKSVINIMMVSGQRNKRRVINYRMLEYTDVTETQ
                     LAAKIFAECNPERFPNITIAIDGDGLGKATADLMYEYYGITVQRIRWGKKMHSREDKS
                     LYFDKRAYANVQAAEAVKSGRMRLDKGNETIEEASKIPVGINSAGQWKVMSKEDMKKK
                     LNLHSPDHWDTYCFAMLADYVPQDEVLSVEDEAQVDEALAWLNECLL"
     gene            10299..10529
                     /locus_tag="ACHOSM_RS17470"
                     /old_locus_tag="pgaptmp_003494"
                     /pseudo
     CDS             10299..10529
                     /locus_tag="ACHOSM_RS17470"
                     /old_locus_tag="pgaptmp_003494"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            10554..10775
                     /locus_tag="ACHOSM_RS17475"
                     /old_locus_tag="pgaptmp_003495"
     CDS             10554..10775
                     /locus_tag="ACHOSM_RS17475"
                     /old_locus_tag="pgaptmp_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4559932.1"
                     /translation="MATIPTPTHPEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPC
                     IFSARFSTDLRNSLSYWSLPVNASAQEGL"
     gene            10772..11887
                     /locus_tag="ACHOSM_RS17480"
                     /old_locus_tag="pgaptmp_003496"
     CDS             10772..11887
                     /locus_tag="ACHOSM_RS17480"
                     /old_locus_tag="pgaptmp_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU4559933.1"
                     /translation="MRTSLVTREEMIEAIEQHTACISTRDIPGVIANYFMITKQLYRR
                     KDKNAVHRILLSDIREYLLEQGHLNYATVAAEARKEAHRMKATNVKSEKIHAPSVQES
                     ELVVVQNQSDEIPVLEWQGVRVVTTKILAKGYGTDADNIKKNFSRNAERFCEGKHFYK
                     VIGDDLNNLRVTLSNSQNPISPKARSLILWTERGAARHAKMLETDQAWAFFEKLEDSY
                     FRQKEQQPVAIPQTLPEALRLAAELAEQKQLLEQKAHQLNQQLVAAAPKVDFADRVSV
                     AKGILIGNFAKVVGLKQNALFVWLRENGILIASGGRKNVPFQQYINAGYFTVKEVVLD
                     DEDGYQIRLTPQLTGKGQQWLTRKLLDAGLLKPVAAE"
     gene            complement(11920..12771)
                     /locus_tag="ACHOSM_RS17485"
                     /old_locus_tag="pgaptmp_003497"
     CDS             complement(11920..12771)
                     /locus_tag="ACHOSM_RS17485"
                     /old_locus_tag="pgaptmp_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein C1"
                     /protein_id="HGU4559934.1"
                     /translation="MINYVYGEQLYQEFVSFRDLFLKKAVARAQHVDTASDGRPVRPV
                     VVLPFKETDSIQAEIDKWTLMARELEQYPDLNIPKTILYPVPNTLRGVRKVTTYQTEA
                     VNSVNMTAGRIIHLIDKDIRIQKSAGINEHSAKYIENLEATKELMKQYPEDEKFRMRV
                     HGFSETMLRVHYISSSPNYNDGKSVSYHVPLCGVFICDETLRDGIIINGEFEKAKFSL
                     YDSIEPIICDRWPQAKIYRLADIENVKKQIAITREEKKVKSAASVTRSRKTKKGQPVN
                     DNPESAQ"
     gene            complement(12882..13091)
                     /locus_tag="ACHOSM_RS17490"
                     /old_locus_tag="pgaptmp_003498"
     CDS             complement(12882..13091)
                     /locus_tag="ACHOSM_RS17490"
                     /old_locus_tag="pgaptmp_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c1 repressor inactivator"
                     /protein_id="HGU4559935.1"
                     /translation="MAFIPPTIDDVRHCSNALSVDPAETDAARAIAEHYSKISNQEYR
                     ITQDDLDDLTDTIEYLMATNQPDSQ"
     gene            13696..13917
                     /locus_tag="ACHOSM_RS17495"
                     /old_locus_tag="pgaptmp_003499"
     CDS             13696..13917
                     /locus_tag="ACHOSM_RS17495"
                     /old_locus_tag="pgaptmp_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
                     /protein_id="HGU4559936.1"
                     /translation="MGYSAAKVSTHIELEKNRGYWRAKGFDRDSCQLSLSRGEEKIER
                     TRGRWRFYDENHKQVKAEPILYTLLKTII"
     gene            13925..14956
                     /locus_tag="ACHOSM_RS17500"
                     /old_locus_tag="pgaptmp_003500"
     CDS             13925..14956
                     /locus_tag="ACHOSM_RS17500"
                     /old_locus_tag="pgaptmp_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGU4559937.1"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKM
                     LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRS
                     GLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRN
                     LAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT
                     KLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAK
                     DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGA
                     MVRLLEGGD"
     gene            15007..15318
                     /locus_tag="ACHOSM_RS17505"
                     /old_locus_tag="pgaptmp_003501"
     CDS             15007..15318
                     /locus_tag="ACHOSM_RS17505"
                     /old_locus_tag="pgaptmp_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogeny establishment protein"
                     /protein_id="HGU4559938.1"
                     /translation="MRKDFNIDGKYVVLSVSTNIQSPAVIVTVKLSDRMPDIDSISVA
                     FPVRSMRSAEHFVMNATEEEARRGFAKVMSEFGEFLGHVDKALSISSAKSKALTASMM
                     K"
     gene            15562..16122
                     /locus_tag="ACHOSM_RS17510"
                     /old_locus_tag="pgaptmp_003502"
     CDS             15562..16122
                     /locus_tag="ACHOSM_RS17510"
                     /old_locus_tag="pgaptmp_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ref family protein"
                     /protein_id="HGU4559939.1"
                     /translation="MKTIEQKIEQRRNWQKAARERAIARQREKLADPAWRESQYQKIR
                     DAIDRRIAKQKERPPASKALKSAVKIKSRGLKGRTPTAEERRIANALGALPCIACYMH
                     GVISEEVSLHHISGRTAPGCHKKQLPLCRWHHQHAAPAEVREKYPWLVPVHADGVVGG
                     KKEFTLLNKSEMELLADAYEMANIMH"
     gene            16214..16936
                     /locus_tag="ACHOSM_RS17515"
                     /old_locus_tag="pgaptmp_003503"
     CDS             16214..16936
                     /locus_tag="ACHOSM_RS17515"
                     /old_locus_tag="pgaptmp_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HGU4559940.1"
                     /translation="MAIHRIPKFFSDVKSVEWPCPTCGQKTLRILEDSFVCKETASTT
                     KMLSDEWLDLEYVSSIFSCMSQCIRQECNEVVACSGRSGLEPDWDEHNGIQYKPWYEP
                     LSFIPGLKLFTLPEKCPVKISAPLEASFTLSKICPGAAANLIRISIEELLTAIDVPNK
                     KGDKLHNRIKAIPDNYSRFKDLLMPIKFLGNAGSHSYEKVTSEDLECSYEIMDFILND
                     IYSGGREMVQELANSLDVKFRT"
     gene            17110..>17569
                     /locus_tag="ACHOSM_RS17520"
                     /old_locus_tag="pgaptmp_003504"
     CDS             17110..>17569
                     /locus_tag="ACHOSM_RS17520"
                     /old_locus_tag="pgaptmp_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturation control protein"
                     /protein_id="HGU4559941.1"
                     /translation="MDKKICVVSMSVGKPASMTAAWINNELIMAERTSYPERRRDMEL
                     QLLRELREKEEKGFIVLVEEENSFITGRVGQRVRLRDPFMNSRPVLIEAMQIYKALER
                     QKAIKLPRKESGKYILHQSIFDSEHDKKGDEFFNINWSEITTVNRPGFPGE"
BASE COUNT         4832 a         3789 c         4518 g         4430 t
ORIGIN      
        1 accgatgctt tcctggctga agtgcgggcg cagggcgtgg atgctgctat agaagctgca
       61 aaaaatctgg tggcccaaga atatgagtat aaggatttca aagcggcgca gagtgattgc
      121 tgtatgcacc ctggttcaga cctggtaggg aaggttgaaa tgactgagtg gttagttgac
      181 tttgctgccc agcttcgcaa aggaggcaac cagtgagcga aattaattac caggcactgc
      241 gtgaggcggc ggaacgtgca attccagcaa tggaacgcct gttaatgttg ccagctgatg
      301 atgatttgtt aagtgaacag gaacttaaag attacggtgt ggatattgat gcgctcaacg
      361 ccttcaaatt tctggccgga ccagaaaccg tgctggcact actggatgaa cgggaaagga
      421 accagcaata catcaaatcc cgcgactagg agaacgagga tattgcgcta acggtaggga
      481 agttgcgcgt tgagcttgag gcagaaaaac agcgggcaaa ggatctgttt atggaaaatg
      541 ctcggcttaa gtcaggtata gccggtctga tacacctcgg tattcgatat gcagatgttg
      601 atgtcatgaa aattgctgga gatgcccagc tttctacccc atgcactgac agcatcataa
      661 acagcattgc aacaggcatt cgcatcaacg gaggtgagta gtgcgtgtgg catgtattgg
      721 cttgttaccg tacccgactc gtttttgggc ttctgcgcta attgcaaagc cacaggtcct
      781 gatggctgac aacatcaccc cagcaccaaa gcgccgccat accggtattg cagcggcacg
      841 acgagcagca aagagacgca ggagagcaaa gcgatgaaaa accgtaaggc aaaacgactt
      901 tttttacagc gacctgtgcg tgtggtggag ctggttatta gcaaccataa gatagcggta
      961 ctccatccat ttggtcaggt ggcttttgcc gcaaagcgta agcctactgc gtcacagaac
     1021 aggcggaaga aagggtacgc agtaagatga aaaaccgtaa agcaaagatt ctgttagttc
     1081 gtagaaacgc tcctggcgtc tggcagtggg tgagactcag caaccgacgg atggggttga
     1141 tgaaatatta cgggatgatg gattgtggtt tttgcaaaaa gcccagcgcg gcgcaaaacc
     1201 gctggaaaaa ccatttgcgc actaaaggag agtgatatgg cgttaacaca ccgcgaactc
     1261 tgtcagattg cgtacaagtt ccttaagcgc aacgggttca aggtttgttt tcatgaccgc
     1321 tttatagctg taaccagtac cggagaacag ccagatgcta tggggttcag aaattcagca
     1381 tcatgcctga tagaggcgaa gtgttctcgt gctgacttgt tggcagatag aaaaaagcgt
     1441 ttccgtaaaa atccctcact tggcatgggc gactggcgat tctttattag tgagccggga
     1501 attatttcag ttgaggattt accacctggc tggggattac ttcacgttgt taacggaaga
     1561 gtacggaaag tacatgggtg gcccaagggt aattgctgtt ggggtaatcc tgacgataag
     1621 ccatttactg gaaataagca ggttgaatgc gattacatgt tgtctgcatt aaggcgcatg
     1681 gagttgagag ggcaccttaa tgaaatatat gacggtgtaa ttgttaataa gaaagaagga
     1741 aacgcggcat gaccactatt accagagagc agttaatagc tcacgcagag gagactattg
     1801 aagcacagag actgtgtata ccgggcacaa tcgaccatga catcatccgc acatataaga
     1861 tggatattgc tgttctggaa atcgcactgg catcgctggc agcagagcca gccggtaaat
     1921 tgcatgaata caaaccagtg ggacatcagc gtctggtcga cgagttaacc atgctggtaa
     1981 agcagttaac ctggcaactg aggaaaacga agccggactg taaattgccg ggtaaggcga
     2041 tggactacct gaagcgaaac ggactgataa gcgcagagga tgttttacga tgacctggcc
     2101 tgaagcattc accacggtag gaattgtgat ggcggcagca ctgggtttgt attcaatttg
     2161 tcgctggtgg taacgatggg aaaaataact tttgtagtcg aatttgagga tggtaaagag
     2221 ccacctgtta gcgccaatct tgatgttgcc ggtggcaggc ttgtttcggt tctatttggt
     2281 gactaccgag atgatttctt ccaaccagaa gaagttgatg tagtgcgaga agcattaaac
     2341 gagctaagtg ttgataacga tgatgctcat gcggaaatca tcaaaaaaat ggaactgcta
     2401 actcactaaa ttatcaatta tggttctatc acctacgaca cagagagaag ttttataatg
     2461 tcaaaagtaa atgttttgat tttttcagta attgtaggtt ttggtttttc tgctggagtg
     2521 catatttata ttacgtgggg aaaaatcatc aactacgtat ggagttgttt tattaagtgg
     2581 ggtaagtatg tggagaggta atagtcatgg aaaaagccag atgatactta ccgaatatca
     2641 gctcgaccac aaaaccaata aatcacgttc agtatatttg cttcgccaca atagccgcgt
     2701 aaggaatacc gtactggagc aaaatctgac cgttgaaatg gataattacg ggggcttcaa
     2761 gccaacaata tcgcttgatg attttcctcg tggtttaagc gaaagagaag caatgctgaa
     2821 attagcagaa tggctacaaa gattaagcat tgctattgaa gataactggt ctgaacctta
     2881 aatttatatg atgacactaa aacattttct tgaccgccca ttatgggcgg cagccgcagg
     2941 ctatgacttt aattatatgg attgcatgtc ttatactgcc aatgcatacg accattcgtt
     3001 cagcctgctg tttaattctt taagaatatt gccgcaaaca gaagttggag agcttcattt
     3061 atggctattg ggctttatcg cggctggagt tggtattgct gtatggcctt ttattttctg
     3121 gctggtggct gttgtagtgt ggtttaagtg caagacgtac cggaaaaagt atttcttagg
     3181 tgatggaatg actgatattg ccaaaatgaa cattgaaaaa tggactaagg aatgtgaaaa
     3241 gaaatggcgc aaaaagaaat gactactcta acgacagcat acttacagca attggtattt
     3301 tttgcaggcg aggctacttg tcatcctgac gcaaactatt tattggaatt tgagaggtta
     3361 gcgtcacccg gtatcgttct ggaactggcc caacaggtgc tggccttaag acaaaaagag
     3421 caacatgaaa gtaatacgtg tagattgaat tttgagcagt ggctggaaca gcaacgcgga
     3481 aaaatcgatg tggactgtgg ttgtgtgtct actgaaacat tcatgcactg gctgcgggta
     3541 gcttacgagg ctggcaacta tccggatatt ccggatagtt cggtgccagc gccaggaaag
     3601 ggcgtcaccg gtgaacgtat ccgcattaag ccgcatgttt atcgcgaact ggttaaccgt
     3661 ctccacgata cagcgatcaa gtgtgcaggc acccggcaat tacgagaaag aattagccgt
     3721 gttttgggcg acgttattac gccagatcat cataaacaag ccgagaaaag tgacctggaa
     3781 aggtgtcacc ttgaggcggc attaaacatt aagccggggc atacgcttgg cattatcgat
     3841 gctctgttgg ttcataagat ggccagggct ttattgccgc tggtggctga aaagcatgag
     3901 gcggaccatg ccaacgaaag ctgagttaca ggcgcgcata gagattcttg aaaaagagaa
     3961 tgcgagtcta aaagtaatgc tggcacgggc ggaaagggaa ttatcaggca aattcttgcc
     4021 agaagaactg ccaccagcag atataccaga tcgagtgtcc cggtggatga agtcttttgg
     4081 tttaccgtgg gaggcttttt ggtgctacga gcatcgcaga tggtgtgatg aacttgatag
     4141 cagtttcccc tactttgcgg aagggaacac ctgccctgag tgtaggaggt agcgcgatgt
     4201 ttcgtcctcg tccaataccg ccgaaaataa ctaatggcta tcagccgaat actccagaaa
     4261 tacaggggag cgaggggctt aaaccaccac cagcaccacg tattccgcct gtagcaagga
     4321 acgtatcatt acgacgagag ttaatattag ccttgtcgcc cgttctggct gcccgccagg
     4381 attgggtaag taatgatgac ctggcagaaa aaatcacatc gctggtggat aaaattctgc
     4441 agattggcaa tgagattacg ctgatccatt ggtgatgaat agtagcaaag cgcacaaaat
     4501 catctgcggt ggttggtgta cataacgagt ttgcaccaaa ggtgtctctt taatgtatgc
     4561 tgtataaatg aacagtatta ttgaggtgaa aacgctatgg gcttcccttc tcctgcggcg
     4621 gattatgttg aaagcagaat ttctcttgat cagcaactaa tcaggcatcc atcagcgacc
     4681 tacttcatgc gggcggcaga cagccatcac cgtgagggaa tattgcaggg tgctttgctg
     4741 gtggttgatt cctcgcttac tccggttgat ggttcgctgc ttgtctgcgc tatggagggt
     4801 gaatatcgca taaagagata ccgaaagtat ccgcgccagc acctggagga tttaagcacc
     4861 gggaagaaag aggcgttacc agtagatgac gatggttaca cgggtagtaa tgctgttttt
     4921 ggtgtgatca ctcatgtcat caatgatgcc cgaagtgggg aatttgatga ttgtccggtg
     4981 atttaagctg caaagtgctg gtgctttatg cctgtgaagt ttataattgt gtacacataa
     5041 cgagtacacg aggtgtttat gcaatccatt aacttccgta ccgcgcgcgg caacctttct
     5101 gaagtgctca acaatgttga agccggggaa gaggttgaaa tcacccgcag aggccgtgag
     5161 ccagcagtaa ttgtcagcaa ggctactttc gaagcctaca aaaaagcggc gctggatgct
     5221 gaatttgcat ccctgtttga caccctggac tccaccaaca aggaactggt taaccgataa
     5281 tgaggcatat atcaccggaa gaacttattg cgcttcatga tgcgaatata agccgctacg
     5341 gcggcctgcc tggcatgtca gatccgggca gggcagaggc cattatcggg agagttcagg
     5401 ccagagttgc ctacgaagag atcaccgacc ttttcgaagt ctccgccacc tacctggtgg
     5461 ctacagcgag agggcatata ttcaatgatg ccaataagcg taccgcgcta aacagcgcgc
     5521 tgctatttct acgccgtaac ggggtgcagg tatttgattc acctgaactg gcagacctta
     5581 ctgtaggcgc tgcgactggc gagatatctg tatcttctgt cgccgacacg ttacgtagat
     5641 tgtatggttc tgcggagtag attaatggca cgcaaataca acaaattgtc ccgtgaagcg
     5701 ttaaagatgc ttcttgatgg cgtgagtcgc cgcgaggtaa agcaatacct ggttggtaag
     5761 caaattggag ccaggactgc tattgctgtg ttatgccgtc aggaaatggt tgtgcttaaa
     5821 cagagaatgc cgggcagcag ataaagccca atcagtgatg aaaggtgtga tgtgaaagcc
     5881 gtaattactc cctttgtaca aaaagagctt ggcgtcgcca cattcaaagt ggatcaggaa
     5941 gtcagaaagc tggtggaggc tggccgtaaa tttattatgg agccggtgcc gcgtgagtta
     6001 atcgagcaca tggacgacgg cctcgttgtt tccgagcaaa ctatggcaac aaatgaggcg
     6061 ttgcagccgt tttttaacag cgatgaactg tttcgccgta ttggtggaat tgactcgctg
     6121 gtggcgtggt tgcgcaggaa agaggggcaa tgccaggccg cagatcgtag ctggtgtgac
     6181 aaccatattg tctacgctga acgagacaat agcgcggtgt tgttgtgctg gcatcacgat
     6241 aaccattacc ggatgcgtgg ttttaatgag ctgaaagaaa cgctgcataa taatcgcgtt
     6301 aactggatac tggatgtcgc ccgtcaggaa atggggcttt cagatggcca tgatttaagt
     6361 attcaggaac tgtgctggtg ggctttcatg cgcaacatga tgcacctgat gccggaagaa
     6421 gtttgccgta tatcaataaa taagatgaaa gccgcaacgc aggatagcgg acctctgaaa
     6481 gaggcggata ttcgcccgta tgacgatcgc gctacagcat atgttcagat gatggaagaa
     6541 cgcgccgcgc cgatgcgtgc aaaagtatgc cctgtggatg ttgactccga ccctggcatg
     6601 gcgcatttca aaataccaaa actgcaatcg ctaaaattac ctgagtacat ggactttgtt
     6661 gcttcccgtc catgctgtgg gtgtggagcg gcgggagctg gcgctcacat tacgccttat
     6721 atcgttcgtc atagtcgatt atgcgcgcat gacatttacg caattcctct gtgccagtca
     6781 tgccagcgtg atattgagcg tgaccgcgat aattgggaga agacgcacgg caggctggcg
     6841 atgcatcaac gattgttctt tgattacgcg cttggagtcg gcgctatcac aagtcactcg
     6901 tcgagcgtta gataaaattg ctctaatgta ttgctatttc tttaatcgag ggtattatat
     6961 tcgtcgttga ttagttgaca tgggctaatc agtaggtgac aggatgttac ttaactggca
     7021 gggacgccac ttcatggaaa taaatcactc acgaattaca tcgtatgaga ttgcggatta
     7081 catgatccgc actaaatctc ttctatcagc gaaagaactc gcagcaatcc ttgaaaagga
     7141 atacccgcat ctggatgtcg ataagcgcga tgtttatctg cgcttaaagg ctatcgctgt
     7201 gtctaagtat tcgtctgttt tgattgatga cagtacacgc ccacgtagat ttcagatcca
     7261 ctctctgaat cctgaattct ttcgccgtag ccgcgctccg cgccggtttg atgaaaaact
     7321 ccagaacgaa ctctatatga cgcaggacga aaaggaacgc cgggagcacc agccttgggt
     7381 aatggcgcgt caacttttca ataaggtggc ccgtcagcac cgtcattacg gtaatgccac
     7441 atccgcacgt atctgattga ttgcttgccc gttccgggcc ttttgacatg tgactttcgt
     7501 taccctcgcg tcaaaaagag tttttacgaa gggaagcata agtgacctgg gacgatcaca
     7561 agaagaattt tgctcgcctg gcgcgagatg gtggttacac catcgcacag tatgccgccg
     7621 agtttaatct taaccctaat actgcacgtc gttatctccg tgccttcaaa gaagacacca
     7681 ggacagcgga cagccgcaag ccaaataagc cagtcaggaa gccactaaaa agcatgatca
     7741 ttgatcactc taatgatcaa catgcaggtg atcacattgc ggctgaaata gcggaaaaac
     7801 aaagggttaa tgccgttgtc agtgccgcag tcgagaatgc gaagcgccaa aataagcgca
     7861 taaatgatcg ttctgatgat catgacgtga tcacccgcgc ccaccggacc ttacgtgatc
     7921 gcctggaacg cgacaccctg gatgatgatg gtgaacgctt tgaattcgaa gctggcgatt
     7981 acctgataga taacgttgaa gcgcggaagg ccgcgcgcgc tatgttgcgt cggtccgggg
     8041 ccgatgttct ggaaaccact attctggaaa agtctctttc tcatctcctt atgctggaga
     8101 acgccaggga tacgtgtatt cgtctggtgc aggaaatgcg cgatcagcaa aaagacgatg
     8161 atgaagggac tccgcctgaa tatcgtatcg cgagcatgct aaacagctgt tccgcgcaga
     8221 taagcagcct gatcaacacc atttacagca tccggaataa ctatcgaaaa gaaagccggg
     8281 aggcggaaaa gcacgcttta tctatggggc aagctggcat tgttaagctg gcatacgaac
     8341 gaaagcgtga aaataactgg tcagtgctgg aagcagctga attcatcgag gcgcatggcg
     8401 gaaaagtgcc gcccctgatg ctggagcaaa tcaaagccga tctgcgtgct cctaagacca
     8461 ataccgatga tgaggaaagg caaacagccg tcggtggccc ttctcttgaa gatctggaca
     8521 aagttgcgcg agaacgggcc gccaaccgcc gcgccgatgc cgcattgtgg attgagcagc
     8581 gtagggaaga aatcgccgat atcgttgata caggcggtta tggagatgtt gatactgaag
     8641 gtgtatcaaa cgacccatgg ctggaacaag acctggacga agacgaggag gaagacgaag
     8701 aagttacccg caagctatac ggggatgatg attaatggcc agaagttgcg taacggatcc
     8761 acgttggcgc gagctggggg cgctatatcg ttatgactgg attgctgccg ctgatgtttt
     8821 gttcggcaaa acacctacct ggcagcagga tctgattatt gagtctgtgc aggaacaggg
     8881 tagcaagaca tctgtttcgt ctggtcacgg taccgggaaa tcagacatga cttctatcat
     8941 gatcatgttg ttcataatca tgtatcccgg tgcccgcgcc attatcgttg cgaacaaaat
     9001 tcagcaggta atgaccggta tattcaagta catcaagata aactgggcta ctgccaccag
     9061 ccgttttcca tggcttgctg attattttgt tctgacagaa accgctttct atgaggttac
     9121 tggtaaaggt gtatggactg tagtaccgaa gggctttcgt ctgggaagtg aagaagctct
     9181 cgccggtgaa cacgcagatc atcttctgta tattatcgat gaagcctccg gtttcagtga
     9241 tagagctttc ggtatcatca ctggtgctct taccggacag gataaccgca tcttattact
     9301 gtcacagcct acacgcccaa gcggctattt ctacgatact caccataaac tggccaagcg
     9361 tcctggtaac cctgatggcg tttatacggc gatcacgctt aacagtgagg aatcaccgct
     9421 ggtaacgcca gcatttatca aaatgaagct ggcggagtac ggcgggcgtg ataaccctat
     9481 gtacatgatt aaggtacgcg gactattccc taaatcacag gatggcttcc ttcttggacg
     9541 tgatgaggtt gaacgtgcga cgcggcggaa agtcaagatt gccaaaggat ggggctggct
     9601 tgcatgtgtg gacgttgctg gtggtacggg acgggataag tccgttatca atatcatgat
     9661 ggtgtccggc cagagaaata aacgccgtgt aatcaactat cgaatgctgg aatacacaga
     9721 cgttacagaa acgcagcttg ccgccaaaat tttcgcagaa tgtaatcctg agcgattccc
     9781 aaatatcacc atagcgatag acggcgatgg gctgggtaaa gcaacggcgg atctgatgta
     9841 cgagtattat ggtattaccg tacagcgtat acgctggggt aaaaagatgc atagccgtga
     9901 agataagagc ctgtactttg ataagcgtgc ttatgccaac gttcaagccg cagaggccgt
     9961 aaaatctggt cgtatgagac tggataaggg taatgaaact attgaggaag cgtcgaaaat
    10021 ccctgtaggg attaactccg caggtcaatg gaaggtgatg agtaaggagg atatgaagaa
    10081 aaaactcaac ctgcactcac cagaccattg ggatacatat tgtttcgcta tgctggcgga
    10141 ttatgttccc caggatgaag tgcttagcgt cgaagacgaa gcgcaggttg atgaagctct
    10201 ggcatggctt aatgaatgtt tgctctaata aattgtgttt tttaattacc gatgttacat
    10261 tgaacctgac ctcttgcgcc ttgaggcatt ttcggtttat gcttatcagg cacctcatta
    10321 aaacgggtgc cgggattggc ctcccgctta agtctaaggc gatacagacg ccgctcgcgt
    10381 ctttttttgt atcggcgtac acgcacacct ctacaatggt gggctgtatg gggctacctt
    10441 cgggtaggct ggttaccttg gacgccagta aggccaactc cgtacagtcc accgccagca
    10501 agattggtct cttctgcggt ggttacatac caacgtctaa ggaggctgcc aatatggcta
    10561 ctatccctac cccaactcat cctgaattta tctggcgctt ttactcctgc caaaaacgtc
    10621 actatcactt cgttattgca ccgacagaag atgaggcccg ctctcagctt cctgacgccc
    10681 catgtatttt ctctgctcgc ttttctaccg atttgcgcaa ttctctcagt tactggagcc
    10741 tccctgttaa cgcttctgct caggaggggt tatgagaacg tcattagtca cccgtgaaga
    10801 gatgatcgag gcaattgaac agcacactgc ctgtatcagt accagggata taccgggcgt
    10861 tattgccaac tacttcatga tcaccaaaca actttaccgg agaaaggaca agaacgcagt
    10921 tcaccgcatc ctgctgtctg atatccgcga atacctgctc gaacagggtc atctgaatta
    10981 cgcaaccgtc gcagccgaag cacgcaagga ggcacacaga atgaaagcaa ctaacgttaa
    11041 atcagaaaaa attcatgcac cttcagttca ggaatcggag ctggtggttg ttcagaatca
    11101 gtctgatgaa attcccgttc tggaatggca gggagtgcgt gtagtgacaa ctaagatcct
    11161 tgctaagggg tatgggacag atgcggataa catcaagaag aacttttccc gtaatgctga
    11221 acgtttttgt gaaggcaagc atttctataa agttattgga gatgatttga acaatttgcg
    11281 ggtgacttta agtaactcac aaaatcctat ctctcctaaa gcccgctcac tcatcctctg
    11341 gacggaacgc ggcgcagccc gccacgctaa aatgctcgaa accgatcagg catgggcatt
    11401 ctttgaaaaa ctggaagaca gctacttccg gcaaaaagaa cagcaaccgg ttgcaatccc
    11461 ccagacgctt ccagaagctc tgcgcctggc tgccgaactg gctgaacaaa agcagcttct
    11521 ggaacagaaa gcccaccagc taaatcagca gctggtggcc gccgctccta aagtcgattt
    11581 tgccgaccgg gtatcagtag ctaaagggat cctgattggg aattttgcaa aggttgttgg
    11641 acttaagcaa aacgcgctgt ttgtctggtt acgggagaac ggcatcctga tagcgtccgg
    11701 tggacgtaaa aatgtgccgt tccagcagta catcaacgcg gggtatttca cggtgaaaga
    11761 agtggtgctg gatgatgaag atggctacca gatacggttg acgcctcaat taacgggtaa
    11821 aggccagcag tggttgacgc gtaaactgct cgatgctggc ttgttaaaac cggtggcggc
    11881 tgaataatgg aagaatgccc ggttgatgcc gggcataatt tattgcgcgc tttcggggtt
    11941 gtcgtttact ggctgcccct tcttggtttt acggctgcgc gtaactgatg cggctgattt
    12001 gacctttttc tcttcgcgag tgatggcaat ttgttttttt acattttcaa tatctgccag
    12061 gcgatatatt tttgcctgcg gccagcggtc gcagatgatc ggttctatag agtcataaag
    12121 gctaaatttt gctttctcga attcaccgtt gatgatgatt ccatcacgga gagtttcatc
    12181 gcagataaac acaccacaca gcggcacatg gtaactaact gatttaccat cattgtagtt
    12241 agggctacta gaaatgtaat ggacgcgcag cattgtttcg ctaaagccgt gtacgcgcat
    12301 acggaatttt tcatcctccg ggtactgctt cattagctct tttgttgctt ccaggttctc
    12361 tatgtatttc gcactgtgct cattgatccc cgcgcttttt tggatgcgaa tgtccttatc
    12421 aatcagatga ataatgcggc cagcggtcat gttgacgctg ttcacagctt ctgtctgata
    12481 agttgtaacc ttacgcacac cgcgaagggt gttaggcact ggatataaaa tagtctttgg
    12541 gatattgagg tctgggtact gttccagttc ccgcgccatt aaagtccatt tatcaatttc
    12601 agcctgaatg ctgtcagttt ctttgaacgg tagaacgaca accgggcgta caggacgacc
    12661 gtcgctggct gtatcaacgt gttgggcgcg tgcaacagct ttttttagaa agagatccct
    12721 gaagctgacg aactcctggt acagttgttc gccgtagaca taatttatca ttgatcctcc
    12781 tccagaattg acatggccaa caactcacag cggattacac tgggagttgt tggccaccat
    12841 tatagaagga tccaacgaaa ataatagatt tattagtgca tttattgtga gtctggctgg
    12901 ttagtggcca tgagatattc gattgtgtca gtgagatcat ccaggtcgtc ttgggtgatg
    12961 cggtactcct gattggatat ctttgagtag tgttcagcaa tggcgcgggc agcgtcggtt
    13021 tcggcggggt ctacagataa agcgttagag caatgtctaa cgtcgtcgat ggttggtgga
    13081 atgaaagcca taattatgcc tcactgtatt gacaacacag agcctgaagc tctgacctac
    13141 tgtttcaccc atgatccatg ctggggtaat ctaacaacat tgcgctgtgt gtaagatgag
    13201 caatgcatag ctgtaatgcc gttgtataag gtttccctgt ttgctcattt ccttctgagc
    13261 cgctctacaa cgctgaagac acattaaata gtgaatccaa agtcgtatta cgaaacggcg
    13321 gcaaaactat aatttattag agcaattgtc aaacaactat gaaaaacaat ccagtttttg
    13381 gctggtggag tgggattttt ctctcaaaat ttattgctct aataattctt gatttttatg
    13441 cgtagctgga cgtaaactcc tcttcagacc taataacttc gtatagcata cattatacga
    13501 agttatctta agggttattg aacatgatca atttacctgt aaatccatac agttcaatac
    13561 cttatcaggt caaatagtga tcacttgatc atttgatcaa ggttgcgcta cgtaaaatct
    13621 gcgaaatgtt ggcagtgtta gtgctccaga tttcgcgtag cgcacttagc accaccaatc
    13681 aatcagaggt gaaaaatggg atattcagct gctaaagtgt ccactcatat tgagcttgag
    13741 aaaaaccgtg gttactggcg ggcaaaaggg tttgatcgtg atagttgtca actgtcatta
    13801 tcgcgcggtg aagagaaaat agaacgcacg cgcggtcgct ggcgtttcta tgacgagaac
    13861 cataaacagg taaaggcaga gccgatcctg tacactttac ttaaaaccat tatctgagtg
    13921 ttaaatgtcc aatttactga ccgtacacca aaatttgcct gcattaccgg tcgatgcaac
    13981 gagtgatgag gttcgcaaga acctgatgga catgttcagg gatcgccagg cgttttctga
    14041 gcatacctgg aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt gcaagttgaa
    14101 taaccggaaa tggtttcccg cagaacctga agatgttcgc gattaccttc tatatcttca
    14161 ggcgcgtggt ctggcagtaa aaactatcca gcaacatttg ggccagctaa acatgcttca
    14221 tcgtcggtcc gggctgccac gaccaagtga cagcaatgct gtttcactgg tcatgcgacg
    14281 gatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa caggcactgg cgttcgaacg
    14341 cactgatttc gaccaggttc gttcactcat ggaaaatagc gatcgctgcc aggatatacg
    14401 taatctggca tttctgggga ttgcttataa caccctgtta cgtatagccg aaattgccag
    14461 gatcagggtt aaagatatct cacgtacaga cggtgggaga atgttaatcc atattggcag
    14521 aacgaaaacg ctggttagta ccgcgggtgt agagaaggca cttagcctgg gggtaactaa
    14581 actggtcgag cgatggattt ctgtctctgg tgtggctgat gatccgaata actacttgtt
    14641 ttgccgtgtc agaaaaaatg gcgtagctgc gccatctgcc acaagccagc tatcaactcg
    14701 cgccctggaa gggatttttg aagcaactca tcgattgatt tacggcgcta aggatgactc
    14761 tggtcagaga tacctggcct ggtctggaca cagtgcccgt gtcggagccg cgcgagatat
    14821 ggcccgcgcc ggagtttcaa taccggagat catgcaagct ggtggctgga ccaacgtaaa
    14881 tattgtcatg aactatatcc gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct
    14941 ggaaggtggc gattagccat taacgcgtaa atgattgctc taattctttg atatttatgg
    15001 cgacatatga gaaaggattt caacatcgac ggaaaatatg tagtgctgtc tgtgagcact
    15061 aatattcagt cgccagccgt cattgtcact gtaaagctga gcgatagaat gcctgatatt
    15121 gactcaatat ccgttgcgtt ccctgtcaga agtatgcgta gtgctgaaca tttcgtgatg
    15181 aatgccaccg aggaagaagc acggcgcggt tttgctaaag tgatgtctga gtttggagaa
    15241 tttttggggc acgttgacaa agccctctca atcagttcag caaagtccaa agcgttaaca
    15301 gcttccatga tgaaataaaa aaagcctggc aagaggccag gctgcacaaa atagcggaga
    15361 ttgtattccg catccaatca atcagaaagg agtatagcac atagatacta aagtgaaaaa
    15421 atgcgactga cgataaaaaa catgacattg ctctaattga ttgttataat taagccgtgg
    15481 cgttagtcaa ctacgaagac atggccatta tctactcctt tgaaatcatt ggcggcctaa
    15541 aggccgcctt ttttttacct tatgaaaaca atcgaacaaa aaattgaaca gcgccgtaat
    15601 tggcagaagg cagccagaga acgagcgatc gcccggcaac gggaaaagtt ggctgatccg
    15661 gcctggcgcg aatcgcaata tcagaaaatc cgggatgcta tcgatcgccg tatcgctaaa
    15721 cagaaagaac gcccgccagc cagcaaagcg ctgaaaagcg cggtaaaaat aaaatctcgg
    15781 ggcttgaagg ggcgaacacc gacggcggag gaacgtcgca tcgccaatgc tcttggcgct
    15841 ctcccctgca ttgcctgcta tatgcatgga gtaatatctg aagaggtgtc tctgcaccat
    15901 atctccggtc gtaccgcgcc gggttgtcac aaaaaacaat tgccactttg tagatggcac
    15961 caccagcatg cagcaccggc tgaagtaaga gaaaaatacc cctggctggt ccctgttcat
    16021 gccgatggcg tggtaggagg caagaaagaa ttcactctgc tgaacaaatc agaaatggag
    16081 ttgctggctg acgcttatga gatggcaaat atcatgcact aatatattta ttattttata
    16141 atcattgctt tgcattatca ttaacacatt gctaacatcg gacttaccta tatcggagga
    16201 gataaatgaa aaaatggcaa tccacagaat tcctaaattc ttttcggatg ttaaatccgt
    16261 agagtggcca tgcccaactt gtgggcaaaa gacgctgaga atattagaag attcctttgt
    16321 ttgtaaggaa acggctagta caacaaaaat gctatcagac gaatggcttg atctggagta
    16381 tgtttcttca atctttagct gtatgtccca gtgtataaga caagaatgta atgaagttgt
    16441 tgcatgctca ggaagaagcg gcttagaacc tgactgggat gaacacaacg gaatccaata
    16501 taaaccttgg tatgaacctt tgagctttat accggggtta aaacttttca ctcttccgga
    16561 aaaatgccct gtaaaaattt ctgccccact ggaggcatcg tttaccctaa gtaagatctg
    16621 cccaggagct gcggcaaatc tgataaggat ttctattgaa gagttactta cagctatcga
    16681 cgtccctaac aaaaaaggtg acaaacttca caatagaatt aaagcaatcc ctgataatta
    16741 ttctcgcttt aaagatctct taatgccgat taaatttctg ggaaatgcag ggagtcatag
    16801 ctatgaaaaa gtcacctcag aagacttaga gtgttcttac gagataatgg atttcatctt
    16861 aaatgatata tattcaggtg gtcgagaaat ggtccaagag ttagctaaca gtttggacgt
    16921 aaaatttcgc acttaaaatg caaaaaaaat tgacatttga caaattactt ctgtcaaaat
    16981 tgcaacacgc ccggtacgga tggatccctt ttcaaatatt ccatggacgg cacagtctga
    17041 gtaccgggcg ctaccttctg ttgtattgct aagccgccgc tggtggcttt tcttttttgt
    17101 aggggtgcta tggataagaa aatatgcgtt gtttcaatga gcgtcggcaa accggcgtca
    17161 atgactgctg catggatcaa caacgagctg ataatggctg agcggaccag ctaccctgaa
    17221 cgccgccgcg atatggaact ccagctgctg cgcgaattgc gagaaaaaga ggaaaagggt
    17281 tttatcgtgc tggtggaaga ggaaaacagc tttattaccg gtcgagttgg ccagcgtgta
    17341 aggttgcgcg atcccttcat gaacagcagg ccggtactaa ttgaagcaat gcagatttac
    17401 aaggcgctgg aacgccagaa agcgatcaag ttaccgcgca aagaatccgg caaatacatc
    17461 ctccaccaaa gcatcttcga ttccgaacac gataaaaaag gcgatgaatt tttcaacatc
    17521 aactggagcg aaataacgac agtgaaccgc cccgggtttc ctggagagt
//
LOCUS       DAZKHC020000085        17568 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZKHC020000085 DAZKHC020000000
VERSION     DAZKHC020000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.085"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            78..1127
                     /gene="allD"
                     /locus_tag="ACHOSM_RS17525"
                     /old_locus_tag="pgaptmp_003505"
     CDS             78..1127
                     /gene="allD"
                     /locus_tag="ACHOSM_RS17525"
                     /old_locus_tag="pgaptmp_003505"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HGU4559942.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1149..2384
                     /gene="allC"
                     /locus_tag="ACHOSM_RS17530"
                     /old_locus_tag="pgaptmp_003506"
     CDS             1149..2384
                     /gene="allC"
                     /locus_tag="ACHOSM_RS17530"
                     /old_locus_tag="pgaptmp_003506"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HGU4559943.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQLK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2395..3180
                     /gene="allE"
                     /locus_tag="ACHOSM_RS17535"
                     /old_locus_tag="pgaptmp_003507"
     CDS             2395..3180
                     /gene="allE"
                     /locus_tag="ACHOSM_RS17535"
                     /old_locus_tag="pgaptmp_003507"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HGU4559944.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILYFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3308..4453)
                     /gene="glxK"
                     /locus_tag="ACHOSM_RS17540"
                     /old_locus_tag="pgaptmp_003508"
     CDS             complement(3308..4453)
                     /gene="glxK"
                     /locus_tag="ACHOSM_RS17540"
                     /old_locus_tag="pgaptmp_003508"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HGU4559945.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNFVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATLDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4475..5776)
                     /gene="ybbY"
                     /locus_tag="ACHOSM_RS17545"
                     /old_locus_tag="pgaptmp_003509"
     CDS             complement(4475..5776)
                     /gene="ybbY"
                     /locus_tag="ACHOSM_RS17545"
                     /old_locus_tag="pgaptmp_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HGU4559946.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(5833..7194)
                     /gene="allB"
                     /locus_tag="ACHOSM_RS17550"
                     /old_locus_tag="pgaptmp_003510"
     CDS             complement(5833..7194)
                     /gene="allB"
                     /locus_tag="ACHOSM_RS17550"
                     /old_locus_tag="pgaptmp_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HGU4559947.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHICHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDNLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(7254..8708)
                     /gene="ybbW"
                     /locus_tag="ACHOSM_RS17555"
                     /old_locus_tag="pgaptmp_003511"
     CDS             complement(7254..8708)
                     /gene="ybbW"
                     /locus_tag="ACHOSM_RS17555"
                     /old_locus_tag="pgaptmp_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HGU4559948.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGKINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(8877..9755)
                     /gene="glxR"
                     /locus_tag="ACHOSM_RS17560"
                     /old_locus_tag="pgaptmp_003512"
     CDS             complement(8877..9755)
                     /gene="glxR"
                     /locus_tag="ACHOSM_RS17560"
                     /old_locus_tag="pgaptmp_003512"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU4559949.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(9855..10631)
                     /gene="hyi"
                     /locus_tag="ACHOSM_RS17565"
                     /old_locus_tag="pgaptmp_003513"
     CDS             complement(9855..10631)
                     /gene="hyi"
                     /locus_tag="ACHOSM_RS17565"
                     /old_locus_tag="pgaptmp_003513"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HGU4559950.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQYGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(10644..12425)
                     /gene="gcl"
                     /locus_tag="ACHOSM_RS17570"
                     /old_locus_tag="pgaptmp_003514"
     CDS             complement(10644..12425)
                     /gene="gcl"
                     /locus_tag="ACHOSM_RS17570"
                     /old_locus_tag="pgaptmp_003514"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HGU4559951.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(12515..13330)
                     /gene="allR"
                     /locus_tag="ACHOSM_RS17575"
                     /old_locus_tag="pgaptmp_003515"
     CDS             complement(12515..13330)
                     /gene="allR"
                     /locus_tag="ACHOSM_RS17575"
                     /old_locus_tag="pgaptmp_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HGU4559952.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCL
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(13408..13890)
                     /gene="allA"
                     /locus_tag="ACHOSM_RS17580"
                     /old_locus_tag="pgaptmp_003516"
     CDS             complement(13408..13890)
                     /gene="allA"
                     /locus_tag="ACHOSM_RS17580"
                     /old_locus_tag="pgaptmp_003516"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HGU4559953.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            14120..15046
                     /gene="allS"
                     /locus_tag="ACHOSM_RS17585"
                     /old_locus_tag="pgaptmp_003517"
     CDS             14120..15046
                     /gene="allS"
                     /locus_tag="ACHOSM_RS17585"
                     /old_locus_tag="pgaptmp_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HGU4559954.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            15115..16209
                     /gene="mnmH"
                     /locus_tag="ACHOSM_RS17590"
                     /old_locus_tag="pgaptmp_003518"
     CDS             15115..16209
                     /gene="mnmH"
                     /locus_tag="ACHOSM_RS17590"
                     /old_locus_tag="pgaptmp_003518"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HGU4559955.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQAIIELSQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            16325..16690
                     /gene="ylbG"
                     /locus_tag="ACHOSM_RS17595"
                     /old_locus_tag="pgaptmp_003519"
                     /pseudo
     CDS             16325..16690
                     /gene="ylbG"
                     /locus_tag="ACHOSM_RS17595"
                     /old_locus_tag="pgaptmp_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(17211..17384)
                     /locus_tag="ACHOSM_RS17600"
                     /old_locus_tag="pgaptmp_003520"
     CDS             complement(17211..17384)
                     /locus_tag="ACHOSM_RS17600"
                     /old_locus_tag="pgaptmp_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559956.1"
                     /translation="MAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFFVGEINVTHYFI
                     TNIGAGLPDACAE"
     gene            complement(17441..>17568)
                     /locus_tag="ACHOSM_RS17605"
                     /old_locus_tag="pgaptmp_003521"
     CDS             complement(17441..>17568)
                     /locus_tag="ACHOSM_RS17605"
                     /old_locus_tag="pgaptmp_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559957.1"
                     /translation="YMAIPENDGVFTWLPDFFPHVAVDISIYTNVEDDYFFLIFP"
BASE COUNT         4621 a         4573 c         4162 g         4212 t
ORIGIN      
        1 tggatcacat aatccagata ttttccctgt atgttaattg cagtcatgct tcacaccgtc
       61 gttaaaaagg aagacagatg aaaatcagtc gggaaacact ccaccagcta attgagaata
      121 aactctgcca ggctgggtta aaacgtgagc acgctgcaac cgtggctgaa gtattggttt
      181 acgccgatgc cagagggatc cactctcatg gcgcggtgcg cgtggaatac tacgcggaac
      241 gcatttcaaa aggcggcacc aaccgcgaac cggagtttcg tcttgaggaa accgggccgt
      301 gctcggcaat tttacatgcc gacaatgccg ccggacaggt cgcggcgaaa atgggtatgg
      361 aacatgccat caaaaccgcc cagcaaaatg gcgttgcggt ggtcggtatc agccggatgg
      421 gtcacagcgg cgcaatctct tattttgtgc agcaggcagc ccgcgccgga ttcattggca
      481 tttcgatgtg ccagtccgat ccaatggtgg tgccgtttgg cggcgcggaa atttactacg
      541 gtactaaccc cctggccttt gccgcgccgg gagaaggcga cgagatcctt acctttgata
      601 tggcgactac cgtacaggca tggggaaaag tgctcgacgc ccgctcgcgt aatatgtcta
      661 tcccggatac ctgggcggtc gataaaaacg gtgcaccaac aaccgatccg ttcgcggtac
      721 atgctctgct ccccgccgcc gggccaaaag ggtatggcct gatgatgatg attgacgtcc
      781 tctcaggcgt cttactcggc ttaccgttcg ggcgacaggt tagttcgatg tatgacgatt
      841 tacacgcagg gcgtaatttg gggcaattac atatagttat taatccgaac tttttctcct
      901 ccagcgaatt attccgtcaa catcttagcc agaccatgcg cgaattaaat gccattaccc
      961 ccgcgcccgg ttttaaccaa gtttattatc ccggacagga tcaggatatt aaacaacgca
     1021 aagccgccgt cgaaggcatc gaaattgttg atgatattta ccagtattta atttccgacg
     1081 cgctttataa cacgtcatac gaaacgaaaa atccctttgc gcaataatta ttgagacagg
     1141 actttcttat gattacacat tttcgtcaag ccatagaaga aacgctgccc tggctttcct
     1201 cttttggcgc tgacccaacg ggtgggatga cccgtttact ttattcgccg gaatggctgg
     1261 aaacccagca gcaattaaaa aaaagaatgg cagcaagtgg gctggaaaca cgtttcgatg
     1321 aagtagggaa tttatacggt cgcctgaatg gtaccgaata tccacaggaa gtgattctga
     1381 gcggttcgca tatcgatacc gtggttaacg gcggtaacct cgacgggcaa ttcggcgcgc
     1441 tggcggcgtg gctggcaatt gactggctga aaacgcaata cggcgcgccg ctacgtacgg
     1501 tcgaagtggt ggcgatggca gaagaagaag gcagccgctt cccgtatgtc ttctggggca
     1561 gtaaaaatat ctttgggctg gcgaatcctg acgacgtgcg gaatatctgt gatgccaaag
     1621 gaaatagttt tgtcgatgcg atgaaggctt gcggatttac tcttccgaac gccccactaa
     1681 ctccgcgtca ggatattaaa gcctttgttg aactgcatat tgaacagggc tgtgtgctgg
     1741 aaagtaatgg gcaatcaatt ggcgtggtga atgcaattgt cgggcagcgt cgttatacgg
     1801 taacgctgaa cggcgaatca aaccatgcag gcaccacgcc gatgggttat cgtcgtgata
     1861 cagtttacgc tttcagtcgc atttgccatc agtcggtcga aaaagcgaaa aggatgggcg
     1921 atccgctggt tctgaccttt ggcaaagtag agccgcgccc gaatacggta aatgtggtgc
     1981 cgggtaaaac cacgttcacc attgattgtc gtcataccga cgctgccgtg ctgcgcgatt
     2041 tcacccaaca gttagaaaac gacatgcggg cgatttgcga tgaaatggac attggtattg
     2101 atatcgattt atggatggac gaagaacccg tgccgatgaa taaggagctg gtcgccaccc
     2161 tgacagaatt gtgtgaaaga gaaaaactga attaccgggt gatgcacagt ggtgccgggc
     2221 acgacgcgca aattttcgcg cctcgcgtac caacctgcat gatttttatc cccagcatca
     2281 acgggatcag ccataacccg gcggaacgca ccaatattac cgaccttgcc gaaggggtca
     2341 aaacgttggc actcatgctt tatcaacttg cctggcagaa ataaggagtc ataaatggga
     2401 tatttaaata acgtcaccgg ttaccgcgaa gatttactgg ctaaccgtgc gattgttaaa
     2461 cacggtaatt tcgcactgtt aaccccggat ggtctggtaa aaaatattat tccgggcttt
     2521 gaaaattgtg acgcgacaat cctctccacg ccaaagctgg gtgcctcttt tgttgattat
     2581 ctggtcacac tgcatcaaaa cggtggcaac caacagggct tcggtggcga aggcattgaa
     2641 acgttcctgt atgtgatctc tggaaatatc actgccaaag ccgaaggcaa aacatttgcc
     2701 ttaagcgaag gtggctatct ttattgccca ccaggctcct taatgacgtt tgttaacgcc
     2761 caggccgaag acagccaaat ctttttatat aagcgtcgct atgttccggt agaaggccat
     2821 gcaccgtggc tggtttctgg caatgccagc gaactggaac gcattcacta tgaaggtatg
     2881 gatgatgtga tcctgctgga ttttctgccc aaagagttag gttttgatat gaacatgcat
     2941 atcctctatt ttgcaccagg tgccagccac ggttatatcg aaacacatgt tcaggaacac
     3001 ggtgcctata ttctttccgg tcagggggtt tataacctcg acaataactg gatcccggtg
     3061 aaaaaaggcg attacatctt tatgggcgct tattctttac aggctggtta tggtgtaggg
     3121 cgtggtgaag cgttcagcta tatttactcg aaagattgca accgcgacgt agagatttaa
     3181 gtcataccac tcgcctgatt atttttagct atgttggatt ttgccacggt ttataccgtg
     3241 gcttttttgt agtaaattca ggtaaaaaaa gatgcgatat aaatcgcatc cgctttaaag
     3301 gtaagggtca gtttttaatt ccctgaccta ttttaatggc gcaggcaata tttcgtgcgc
     3361 tgttgaagag attggtttca ccgctggcga gtacttcggc taaaggtgcc agacgtggca
     3421 aaatgctgaa taccgcatca atgccgtact ggtgcaccac ttccacgcca tcacccaata
     3481 cgccagcaat cccaatcacc ggtacattaa actgcttcgc caccgacgcc acacccagcg
     3541 gcgctttacc gcctgccgtt tgcgagtcga tgcgcccttc cccggtgatc accagtgctg
     3601 caccctgcac tgcctgcgca agattgaccg cattcaacac aatctcaata cccggtttaa
     3661 tatccgcatt gagaaatacc gccgccgcaa tacccatacc gcccgcagcg ccgccgcccg
     3721 ccatctggca gacattaatt tccgtttgct gttgtaaaac acgggcgtaa ttttgcagcc
     3781 cctgttccag ttcttcgacc atttccggcg ttgccccttt ttgcgggcca aataccgccg
     3841 ccgcgccgcg tgcccctacc agcgggttat caacgtcaca tgccacttca atatggctgt
     3901 tcgccagacg cggatcgcat tcctccatct caatgcttgc cacgcgcgct aaattgccgc
     3961 cgttagccgc cagcacctgg ccgtcggcat caaggaaacg cacaccaagc gcctgcgcca
     4021 tgcccatacc gccgtcgagc gtcgcactgc cgccaatgcc gagaataata tggcgaatgc
     4081 cgttatccag cgcatgacga attaactccc ccgtaccaaa actggaggcc agcaacggat
     4141 tacgcttttc aggcgcgacc agcatcaggc cacttgccgc tgccatctca atcaccgctg
     4201 ttttcccgtc gccggtaagg ccataaaaag cattcacttt ttcgcccatc ggcccgcaga
     4261 cttcaagcgt cacgaagttg ccacccgtcg cggcgaccat cgcatccacc gtcccttcgc
     4321 cgccatccgc tatcggcaaa cagatatagt gcgcatcggg aaagagggtc gaaaacccgg
     4381 ctttaattgc ctggcaacat ttttctgcac ttaagctctc tttaaaagag tctggcgcaa
     4441 tgacaatctt cattatcaat cctttttcaa caaattattc gatgcgttcc cacggaataa
     4501 gattatccat aagaacagcc agtaaaatcc cgaccaataa gccgttactg agcagaggac
     4561 gcagcgttaa tggaaggtct tgcagataca caggcggtaa tgccattaaa aatatgccga
     4621 caaataacgg caatgcgagt cgataaatat tgcgagcggt aaaggttatt tgctggctaa
     4681 acaccagcgc ggaaaagagt aaaggcagat aagaaaccag catgaccgcg ctactcacgg
     4741 gtaaagggat actgcaaaac agtcgtgtga gtgccggaac cagcgccacc agcaggcaaa
     4801 taacgctacc ataaataaac gaacgccgcg tgtaatcgcc tgtctgggtt aataaaccaa
     4861 tggatgaaac aaacggtgaa aatggaatta ccgccagcgg tacggttatc agtgtcataa
     4921 atccggtcgc cacaaagcta cgacgataac gcgtattccc cgcgccctgc tgcggataaa
     4981 aaacatccgt gccccgaatc gcaccgtagg tattgctgat atttaccaga cctgtaatca
     5041 ccgccgtcag aataatcccc ggcgacaaag caccgccact gccgagcggg aaccactgcc
     5101 agtgcaactc accggagagc gagtgcgaag aaggaaagca aaagtaccac aacaaccagc
     5161 cggttatggt gccgactagc aggccataac gggcaaaacg ttgcggcagg aagataatca
     5221 tccccagtac caggcacatc accgccaccg agagcgcgaa cggtggtaac tgaattttaa
     5281 aatttgggtc ggctatgcca aacggcagtc cgagcatacc tttgaaaaag atagtggtca
     5341 gctgcgcgcc cagcatcaac ataaacaaga ccatcaccga cggcgtaaat aaccgtgcca
     5401 ggcgatggcc taatccgcta aaaccaatca acatcgtcag cacaccggag agtgcaatcc
     5461 ccaccgccag actggtagcg atatcgttga tcggtgtccc gcgcgatgct tcgccaaggg
     5521 tgatagtaag gatggttccc caccacaggc cacctggccc ttccataata gcgcgacgat
     5581 gaccgcagaa cgcctgagcg aagcaggcca gtgcggtagc aagaaaagca tattgcgtga
     5641 gtgtaagcag gctactttgc ggcaactgaa aagcagaaag tagcgtagga ggaaccacaa
     5701 ccgtgttgca aaaaataaag aaaaaccact gaaatcctga taacaggctt tcgcggctga
     5761 ctgcaaaatt gaacataaac acaccttaac cggagaatgc ccgccgcaag gacgggcatt
     5821 gcaggggcca gattactgct gatgtttaag gataaattga cctttcggcg caacagggaa
     5881 gccctgttca atatcgtaaa tcacatcacc acgtaagatg gttttcgtga tacgcgcgcc
     5941 aatggtacgg ccaacatacg ggctgacttt gtggcgatat tccagattgt cattggtaag
     6001 aacatagctg ctattcggct gaatgaagac gaagtcggca tcttttcctg gggcgatacg
     6061 gcctttttgc tgcagaccga aaatatctgc tgcgttagtc gccattaatt tgccgaacat
     6121 tggcagagac attccgcgtt tctgtaccgc ttcatcgaac atcacgtcca tacagttttg
     6181 cagaccggca ataccgcccc atgcttccat gatgttgccg gctttcattt ccggagggca
     6241 tggtgagtgg tcggaaacca ggcagtcgat ttcaccgtta aacagttttt cccacatgcc
     6301 tttctggttt tccagatcgc ggatcggcgg tgaacacttc gccagagtac cgatttcttc
     6361 gaactgatcg gtatccagta caaagtaatg cgggcaggat tcacaggtaa catcctgacc
     6421 ttcctgacgt gcacgagtca cttcttcaac accttccggg ctgctgatat ggcaaatgtg
     6481 cagacggcaa ccggcaactt tcgccaggta cagtacgcgg cgaatcgctt ccacttcggt
     6541 aaataccgga cgcgaagcca catagtcatg ggcagttacg cgaccttcac gtttcgcttc
     6601 ttcgcccagt gcgtcacaga tcagcgcgtt ttcgcagtgc accagcaccg gctgccccag
     6661 ttcgcccagc ttctgcgcgc ctttgaagaa ctgccagtcg tttacatcac ggaagtcgtt
     6721 gtcgataccg cgatcgccac aggtcgcaac gaagcatttg aagccgacaa cgcccacttc
     6781 atccaactca tgcagacgat caatgttgta agacaccagg ccaccgagtt gtgccgcatc
     6841 gatagtcagc ttgcctttag cggcatcgaa cttcagttca attgaagcac ggtcaaccgt
     6901 tgcaggcagc tgattgagcg gcatttcgat catggtggtg ataccacctt ttgctgctgc
     6961 gcgagtaccg gtttcataac cttcccagtg gctacgacct ggttcagaaa tatgggtgtg
     7021 cgcatcaacc atgcccggcg aaaccaccag accagacgca tccataactt cttttgcatc
     7081 gcccagatcc tgaccgatag cagcaatttt tccgccttta acggcgatat ctacaacgcg
     7141 agcttcgttt tctaaaataa cggtgccgtt tttaatgatt aaatcaaaag acataacaaa
     7201 ctctttgtaa aatacagatt gttttttaac tcaatgttca atatcgggat taattaacct
     7261 atggtttttt gctctcctgt tttttctgct gttgtacgtt tctttaataa ggcgtaggcc
     7321 gcaaaggcga cgatgacgcc aacaaaccat gaaacacgcg ataacggttc cataaaggga
     7381 ataaacttac cgccaagaga taaaataacg gccaccagag ttactgaaaa cgcagtgagg
     7441 ttaaaaccgt tatcgtaata tttataatcg ccaggtgcgg tatacagttc atcaagatta
     7501 attttcccac gcatcaccac aaaataatgc gccatcatga caccaattac cggaccaagc
     7561 attccgccga taatatcgag gaacagataa atactgtcct gattttccat taatttccac
     7621 gggcagatca gcaggctgat aatactggca atcagtacgc cgtttttata ggtcagtttc
     7681 gtcggtgcaa tagcggcaat ctgataaccg gcaggaataa tattaccggt ggcgttagtg
     7741 gagatagtcg tcatcagaat aaccagtacc gcaaagaacg aggcgaacaa gctgtcccaa
     7801 cgctgaacaa tatccagcac gttccaggta tcagcgccgt aatgaatact ggctccggca
     7861 ataatacata cccccgcgac cgcaaacaga atataggcca caactaaacc cagcgtttgc
     7921 cccagcgctt gctcacgaaa cgagtgggcg ttttgcgtaa agtcggatgc gctcaccgcc
     7981 ggtgccgccc agaccgcaac taccgcgttt atcaccacca ggaacaggaa gccactgttt
     8041 tctgctttct gaataccgct cggaatgtag tcaaagattg gaccgagccc gaccagcgaa
     8101 atcgcccaaa tcgccatacc gccgaaaacg atatagatgc acgggttaag aatggcagtg
     8161 aatttattta aaactttgcc accgccaaaa cctataccaa cgttgaccag ccagaagagt
     8221 aagaaagtaa ttaagcccgg tagagaaagc cctaacagcg tgaaatcacc accgagagtt
     8281 aaaaatcccg gccagatttt gccaatcaga atcaagcagg ccagtgaccc cgcgtaacat
     8341 tgcaggccaa accacataat ggcagcaata ccgcctctta ataatccggg aaacagtgcg
     8401 ccacgtacgc cgtaagaagc acgcaggatc atggcaaacg gtacgccgta tttactgccc
     8461 gcagcgccgt ttaataccat taccgcggca atgaaaaagg cgctgaggat aattgccagc
     8521 ataatactaa aggtagacaa gccgagaata aaaaagccgc cgaccatcac ataattggga
     8581 acgttatgaa ccgaacccat ccataaggta aaatagttaa atgttttcca ggtccgctgg
     8641 ctttgcgttt tcggcaatag atcttcgcta tagccgcgtt gctggaatag ttttctctga
     8701 tgttccataa atccctctta atttcacgca tcgaataacg ttgttttaat ctagctatga
     8761 ggaacaaata aaataaaaat attattttta atactgaagc aacatctttg gctgctttct
     8821 tttttaatat tgctggccga ttcattttct gatatcggcc atttttattg cgggtatcag
     8881 gccagtttat ggttagccat taattccagc gcctgcacta acgcagagtg atccaactgg
     8941 ctgccaccgt ttgccgcaca ggtattaaat aactcctggc aggtcgcagt gtttggcagg
     9001 ttcagcgcaa gtgctttcgc actttgcagt gccaggttga gatctttctg gtgcagagcg
     9061 attttgaagc ccggattaaa ggtgcgttta atcatacgct cgccatgaac ttccagaata
     9121 cgtgaggaag caaagccgcc catcagcgcc tggcgcacac gtaccgggtc cgcaccggct
     9181 tttgaagcaa atagcagggc ttcagaaacc gcttcaatat tgagcgccac gataatctga
     9241 tttgccactt tgcaggtttg accgtcgccg ttaccgccca cgagggtgat atttttaccg
     9301 agcagttcaa acagcggttt aacacgttca aataccgctt catcaccgcc aaccataatc
     9361 gacaacgtcc cttcacgcgc accgatttca ccgccggaga ctggcgcatc gagataatcg
     9421 ccgcccagtt cattcacctg acgagcgaaa cgcttagttt caatcgggga aatggagctc
     9481 atatcaacaa tggttttgcc cttcagcgag gctttggtac aaccattttc accgaacaga
     9541 acttcttcaa cctgtggtgt atctggcacc ataataaaaa tgatgtccga tgcttccgtt
     9601 acctggcgcg cagtttcaac actgacggca cccagtgaca gtaattcatc agcaaccgga
     9661 ccaatggttg tgacatgcag ttgatgaccg gcacgcgcca gattaatggc catcggtgta
     9721 cccataatgc ctaagccaat aaatcccagt ttcataatta acctctttta aatttcgctt
     9781 ttcctgaatt cagacaacac gatcccctgc caacttagcg gcctaaaaaa gcattgcctg
     9841 aacagcgtta cgttttaacg gtacggatcc atccagcgta aaccggcttc cgtggtggtt
     9901 tggggtttat attcacaccc aacccaaccg ttgtagtcag aattttcgat taccttaaag
     9961 agataatcat aattaatttc tccggtcccc ggttcgccgc gatgcggatt atcagcaatt
    10021 tgcaggtgac caattttatc ggcccactga gtcatggtgt tggttaattc accttccatc
    10081 cgctgcatgt gataaatgtc atactgaatt ttcaaattgc agcaaccaac atcatcaatc
    10141 aatttcagcg cctgccgagt tccggtgaga tggaaaccag gaatatcaaa atggttaata
    10201 ggttcaatca ataataaaat atcttctttc atcagcatat tcgcggcata acgcaggttt
    10261 tctacaagcg ttgcgtgaat ctgttcactg ctgaaaccag ccggcgtttt accgaccaga
    10321 cagttaattt ttttattccc cagcgcacgg gcataacgaa tcgctgctgc tacgccatcc
    10381 cgaaactctt cttcacggcc aggaatacag gcaataccgc gctcccccgc cgcccagtca
    10441 cccgccggta aattgtgtag cgtatgttcg agtttattac tcgccagcac ctgttttaat
    10501 tcttcaatgt cgtagtcata aggaaacata aattcaacgc cgcgaaaacc atactgcgca
    10561 gctttctcaa aacgggcgag aaaatcatat tctccaaata acatcgataa attagcagag
    10621 aaacgtaaca ttatttatct cccttattca tagtgcatga agcaggtttc agtcggtgcg
    10681 tccgctgcgt tatcggcgat atcttcaaat tccatgacgt tatccagttc gctgcccatc
    10741 gaaatattgg tcacacgctc gagaataact tccacgacta ccggtacccg atattgcgcc
    10801 attaaggctt tcgcctgttc aaaggctggc gcaatatctt ccggtttgaa gacgcgaatc
    10861 gctttacaac ctaaaccttc cgctactttt acgtggtcga cgccgtaacc gttcacttcg
    10921 ctggagttga tattctcgaa agcgagttgc acgcagtagt ccatatcaaa cgcgcgctgc
    10981 gactggcgaa tcagccccag ataagcgttg ttgaccagca catggatgta cggaatgttg
    11041 aactgcgcgc caacagctaa ctcttcaatc aggaactgga agtcaaagtc gccagaaatc
    11101 gccaccacat tgcgtttcgg atcagcggca caaaccccta gcgcagccgg aatcgtccag
    11161 cctaacggac cagcctgacc acagttgatc cagtggcggt ctttaaagac atgcagcatt
    11221 tgtgccgcag cgatttgtga cagaccaatg gtggtgacat agcaaacatc gcgaccaaag
    11281 gctttgttca tctcttcata cacgcgctgc ggtttcaccg gcacgttgtc gaaatgggtt
    11341 ttgcgcagca aagtgcgttt gcgctgctgg cagtcggcga cccattcttt acgacacggc
    11401 agacgacccg ctttttgcat ctcctgcgcc acttcaacca gcagtgtcag cgccgcttta
    11461 gcatcagaga caatgccgag atccggacac agcacgcgac caatttgcgt cggctcaata
    11521 tcgatatgaa cgattttgcg cccttcggtg tatttctcta ccgaaccggt atgacggtta
    11581 gcaaaacggt taccgatacc aaacaccatg tcggacgcca gcaacgttgc gttaccgtaa
    11641 cgatgcgcgg tttgcagacc gaccatcccg gccatcagtt catgatcgtc cgggatacag
    11701 ccccagccca tcagcgttgg gatcaccgga acgctggtca gttcagcaaa ctgttgtaac
    11761 agtgcagctg cgtcagcatt gattaccccg cccccggcaa caatcaccgg acgttcggcc
    11821 tggattaaca tttctacagc tttttcgatc tgcatacggc tggcagcagg tttgtagacc
    11881 ggcagcggtt cgtacatgtc aggatcaaac tcgatttccg caacctgaac gtcgaacggt
    11941 aaatccacca gtaccggacc cggacgacca gaacgcatca ggtgaaatgc ctgttgcagc
    12001 acgcgaggca ccagcgccgc ttcacgaact gtaaccgcca ttttgctgac cggtttagca
    12061 attgcttcaa tatctacggc ctgaaaatct tctttatgca gacgggcgcg cggtgcctgg
    12121 ccggtaatgc acagaatagg aatggaatca gcagaagcgg aatagagcgc ggtgatcatg
    12181 tccgtgcccg caggaccgga agtccccaga catacgccga tatttcctgc cgttgcgcgg
    12241 gtataacctt ccgccatgtg cgaagcacct tccacatgac gcgccagaat gtgacgaata
    12301 ccgccgtgct tacgcatcgc tgagtagaac ggattgattg cagctcccgg aacaccgaag
    12361 gcggtggtga taccttcttt ctccagcaca tacattgccg cgtcaacggc tctcattttt
    12421 gccattttat tcctacctct atttattgga aaatttttcc aactttcaaa ttttgtcaaa
    12481 aaaacccgct ccgcagagcg ggatgcgaca gacattatgg atgtgctttc agtcccaacg
    12541 ccgtgctgat atcgcgggcg gtgtctctga ccagctcacc ctgactgaca aaacgatctt
    12601 ctgtcagtct tgatgaaggc ccggagatgg agatagcggc aacaacacta cctacatcat
    12661 cgtaaattgc tgaagctaag caattcagac ctacaacatg ctcttcttta tctacggtat
    12721 agcccagttc acgcgcttgt tccaggtcct tcagcaaggt gggcatatcc acaagcgtag
    12781 ttggcgtaaa ctgctgcaaa ccggtttgca gaatgatgct catcaactcc tcttccgcca
    12841 gcggataaag cagcgctttg cccgcaccgg aagcatgcag cggcagacga ctgcccagtg
    12901 gcgcacacat cctgaccatc gatttacact ctaactgacc aattaatact gcttcattgc
    12961 cgttacggat cgcgacattg accgtttcgc cggaaagtaa catcaggcgg cgcataaacg
    13021 gcccggcgac ggagaggaca tcgcggttat ggatgtacgc tgcaccgacg ttaaagacac
    13081 ctaatcctat atgccaccag cctaactgac tgtcctgata gacaaaatcc gctgcctgta
    13141 aaaccttcag caagcgaaag gtcgtggaga gcggcaaatc cagattgaga gaaatatcgc
    13201 taaccgacga gcttccccca cttttttcca gatattgcag aatcgcaatt ccccgctcta
    13261 acgcctgtgc gcccttctgt gccacaggct ccgcctgtcc tggcctgccg cgccgtctaa
    13321 cttccgtcat acctacgtta actccgacct acaagtgagc ataaacaatg aacaattagt
    13381 ttatcttatg caagtcggtt gcaggcgtca cgcaaaacag agttcctgtt caggaatact
    13441 ttcaacatca cagttgtcac tgccgccgcg atcgatggtc agaaaatcgg tgacgcgctg
    13501 ccaggcgaaa agtgggtgat gccagacgtt acgatggtaa ttcactccct gttcgccgtt
    13561 ggtgataaac gcccgcagcg ttgacaggtc tggtttgtcg tcgcctaacg ccacgaccac
    13621 cacaaacact tcacctttca tcgggataaa ggcctgagtg cccagcggat gacgttcgag
    13681 ttcgtgaatg gtcagcggca gattcgccgg ttgcgcgcgg ttaatgctga taagcgtacg
    13741 gtcttgctca agaatctcaa ccagcgccaa atcgtggtaa cgctccacca ggccattgtt
    13801 aatatggaaa aaatcccgtt gctgagtttc gattacgtcg ccataagcac taaaggcttc
    13861 ctgacttaac ggtaatacct gaagtttcat ctttttgtcc ttgtaacata attccgttca
    13921 accaccgccg accggtcagc ttggtttcaa taacaggcaa tctaaaacct taagagaaca
    13981 acatcaatac aaaagttgga atatttttcc aaaactagat ttagtccaca aattgaacag
    14041 ggttatcatg taaaaatact gataaatcag aggataagaa aatcacgcaa cttgcggatt
    14101 taaaagggga tacctgaaga tgttcgatcc agaaaccttg cggactttca ttgcggttgc
    14161 tgaaacagga agtttttcaa aagcggcaga acgattatgt aaaaccacgg cgacgatcag
    14221 ttatcgcatt aaacttctgg aagagaatac cggagtagcg ctgtttttcc gcacgactcg
    14281 cagcgtgacg ttgacagcgg ctggcgagca tctactttcc caggccagag actggctgag
    14341 ctggctggaa agtatgccaa gcgagctgca acaggtgaat gatggcgtgg aacgccaggt
    14401 gaatattgtc atcaacaacc tgctctacaa cccccaggcc gtcgcccagt tgctggcgtg
    14461 gctgaatgaa cgttacccct ttacccagtt tcacatctcc cgacaaatct atatgggcgt
    14521 ctgggattcg ctattatacg aaggtttttc actggctatc ggcgtcacgg gaaccgaggc
    14581 gctggcaaat acctttagtc ttgatccctt aggatcggtg caatggcgct ttgtcatggc
    14641 ggcggatcat ccgctggcga acgttgaaga gccgctaaca gaagcgcagt tgcggcgctt
    14701 tccggcggtc aatattgaag acagcgcccg caccttaacc aaacgcgtcg cctggcgatt
    14761 gccagggcaa aaagagatta ttgttcctga tatggaaacg aaaatcgccg cccatcttgc
    14821 gggcgttggc attggttttt tgccaaaatc gctttgccag tcaatgatcg ataatcaaca
    14881 actggttagc cgggtaatcc caacgatgcg ccctccttcg ccattgagtc tggcatggcg
    14941 caaatttggc agcggcaaag cggtagaaga tattgtgacc ttgtttaccc agcgcaggcc
    15001 ggaaatcagc ggatttttag aaattttcgg caacccacgc agttaaaaat caagtttcct
    15061 tgcgcgttgg cgcgtaaaat agcgcgcaag ctgatttgag gttcactttt aactatgcaa
    15121 gagagacaca cggaacagga ctatcgtgcc ctgctgattg ctgatacgcc cattattgat
    15181 gttcgcgccc ctatcgagtt tgagcaaggc gcaatgcccg ccgctatcaa tctgccgtta
    15241 atgaataacg atgaacgcgc cgccgttggc acctgctata aacagcaagg ctcagacgca
    15301 gcgctggcgc tggggcataa actggtggcg ggtgaaattc gtcagcagcg catggacgcc
    15361 tggcgggcag cgtgcctgca aaatccgcaa ggtattctct gctgcgcccg tggcggtcaa
    15421 cgttcacata ttgtgcaacg ctggctgcat gaagcgggga ttgattatcc gctggtggaa
    15481 ggcggttata aggcactgcg ccagaccgcg attcaggcga tcattgaact gtcacaaaaa
    15541 ccgatagtgc tgattggcgg ttgtaccggc agcggtaaaa cgctattagt gcaacaacag
    15601 ccaaacggtg ttgatctgga agggctggcg cgtcatcgcg gttcggcgtt tggtcgcacg
    15661 ttacaaccac aacttagcca ggcgagtttt gaaaacctgc tggctgccga aatgctaaaa
    15721 accgacgccc gtcaggattt gcgcctgtgg gtgctggaag atgaaagccg gatgatcggt
    15781 tcgaatcacc tgccggaatg tctgcgcgag cgaatgactc aggcggcgat tgcggtggta
    15841 gaagatccgt ttgagatccg tcttgaacgc ctgaacgaag agtatttctt gcgtatgcat
    15901 catgatttta cccacgctta tggcgacgaa cagggctggc aggaatattg cgaatacctg
    15961 catcacggac tttcggcgat taagcgtcgg ctggggttac agcgctataa cgaactcgct
    16021 gcccagctgg atacggcgct gacaacgcaa ctcaccaccg gcagcaccga cggtcatctg
    16081 gcctggctgg tgccgttact taaagaatat tacgacccaa tgtatcgcta tcagcttgag
    16141 aaaaaagcgg aaaaagttgt ctttcgcggt gagtgggcgg aagtggcgga atgggttaag
    16201 gcgcggtaat gagggacgta tttgtattag tcgcgacttg atctgacaca tggccttgaa
    16261 aggttgagag ttaccggttt tgatatgagt gtcgaatcct tatccaaaac aagaggtaac
    16321 tctcatgctt catactgcca atcccgtcat caaacacaaa gccggtttgc tcaatctggc
    16381 tgaagaactc agcaacgtat caaaagcctg taaaatcatg ggcgtttcgc gtgatacgtt
    16441 ttatcgttac cgcgaactgg tcgctgaagg cggtgtggat gcgcagatta atcgtagtcg
    16501 ccgcgctcct aaccttaaga accgtaccga tgaggcaact gaacaggctg ttgttgatta
    16561 cgccgtcgct ttcccgacac atggtcagca ccgggccagc aacgagctgc gtaaacaggg
    16621 ggtttttatc tccggcagtg gtgtccgttc tgtctggtta ctccataacc ttgagaatct
    16681 caaaagacga tactgaacaa attttatcag gtgacgttcc gtaaaaagtt gtatggagtt
    16741 ctcgatgcag tcggcgcgac tatcagtacc cccgctgaac cctggactat cgggccgccg
    16801 tactggggca tgtcaccctg gcgtgccgct ggttttccgc taatggttta tttcctcttt
    16861 ctttcccggc aagaatttat tttaatccgg aaggattatt tattttcaga tagggactca
    16921 cattaaacac gtaacatttt aattaatgca tggctgaata tgtattacac aatgtcagat
    16981 atcgtaaaag accagtgcaa ttctatgtaa actctctgac tgttcatttt atttgttgtt
    17041 tcagggtcgg aactcaccct caataaacaa ctgaatacga gtagatattc atcgtctgag
    17101 ctatatggct ttacacaata gccgacatgt cttttctcag tcaggaattt tacttttcct
    17161 cactacgaag ttgacgactt cgatatggga tagactctta attcaagcaa ttactctgca
    17221 caagcatcag gcaatccagc tccaatattt gttataaaat aatgcgtaac attaatttca
    17281 ccaacaaaaa aaaggtttac accatcccct tgccctggta tcttaccata tttacaccac
    17341 actttttttg tatctggatc attctttgat acgattttcg ccacttgttc cctggccctc
    17401 actgtcaatg ttttcttaaa cctaccccca tcatcatttg ttagggaaaa ataagaaaaa
    17461 aataatcatc ttctacattt gtgtatattg atatatccac cgctacgtgc ggaaaaaaat
    17521 ctgggagcca tgtaaaaact ccatcatttt ctggtattgc catatagt
//
LOCUS       DAZKHC020000086        17256 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZKHC020000086 DAZKHC020000000
VERSION     DAZKHC020000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.086"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..917)
                     /gene="casA"
                     /locus_tag="ACHOSM_RS17610"
                     /old_locus_tag="pgaptmp_003522"
                     /gene_synonym="cse1"
     CDS             complement(<1..917)
                     /gene="casA"
                     /locus_tag="ACHOSM_RS17610"
                     /old_locus_tag="pgaptmp_003522"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HGU4559958.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALQSLEHAFQFGPDSPSFM
                     QDFDELKVKATSIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPRLPLKEG"
     gene            complement(1015..3714)
                     /locus_tag="ACHOSM_RS17615"
                     /old_locus_tag="pgaptmp_003523"
     CDS             complement(1015..3714)
                     /locus_tag="ACHOSM_RS17615"
                     /old_locus_tag="pgaptmp_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HGU4559959.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKEGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSLESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQ
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKY
                     CSLQRASQVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEGVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            complement(3907..4059)
                     /locus_tag="ACHOSM_RS17620"
                     /old_locus_tag="pgaptmp_003524"
     CDS             complement(3907..4059)
                     /locus_tag="ACHOSM_RS17620"
                     /old_locus_tag="pgaptmp_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4559960.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(4324..5058)
                     /gene="cysH"
                     /locus_tag="ACHOSM_RS17625"
                     /old_locus_tag="pgaptmp_003525"
     CDS             complement(4324..5058)
                     /gene="cysH"
                     /locus_tag="ACHOSM_RS17625"
                     /old_locus_tag="pgaptmp_003525"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU4559961.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(5133..6845)
                     /gene="cysI"
                     /locus_tag="ACHOSM_RS17630"
                     /old_locus_tag="pgaptmp_003526"
     CDS             complement(5133..6845)
                     /gene="cysI"
                     /locus_tag="ACHOSM_RS17630"
                     /old_locus_tag="pgaptmp_003526"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HGU4559962.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(6845..8644)
                     /gene="cysJ"
                     /locus_tag="ACHOSM_RS17635"
                     /old_locus_tag="pgaptmp_003527"
     CDS             complement(6845..8644)
                     /gene="cysJ"
                     /locus_tag="ACHOSM_RS17635"
                     /old_locus_tag="pgaptmp_003527"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HGU4559963.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYLAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8963..9325
                     /gene="queD"
                     /locus_tag="ACHOSM_RS17640"
                     /old_locus_tag="pgaptmp_003528"
     CDS             8963..9325
                     /gene="queD"
                     /locus_tag="ACHOSM_RS17640"
                     /old_locus_tag="pgaptmp_003528"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HGU4559964.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            9403..10674
                     /gene="ygcN"
                     /locus_tag="ACHOSM_RS17645"
                     /old_locus_tag="pgaptmp_003529"
     CDS             9403..10674
                     /gene="ygcN"
                     /locus_tag="ACHOSM_RS17645"
                     /old_locus_tag="pgaptmp_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4559965.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLEHAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVISGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDILQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            10665..10925
                     /gene="ygcO"
                     /locus_tag="ACHOSM_RS17650"
                     /old_locus_tag="pgaptmp_003530"
     CDS             10665..10925
                     /gene="ygcO"
                     /locus_tag="ACHOSM_RS17650"
                     /old_locus_tag="pgaptmp_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU4559966.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            10942..11517
                     /gene="ygcP"
                     /locus_tag="ACHOSM_RS17655"
                     /old_locus_tag="pgaptmp_003531"
     CDS             10942..11517
                     /gene="ygcP"
                     /locus_tag="ACHOSM_RS17655"
                     /old_locus_tag="pgaptmp_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HGU4559967.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(11665..12525)
                     /gene="ygcQ"
                     /locus_tag="ACHOSM_RS17660"
                     /old_locus_tag="pgaptmp_003532"
     CDS             complement(11665..12525)
                     /gene="ygcQ"
                     /locus_tag="ACHOSM_RS17660"
                     /old_locus_tag="pgaptmp_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGU4559968.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(12522..13301)
                     /gene="ygcR"
                     /locus_tag="ACHOSM_RS17665"
                     /old_locus_tag="pgaptmp_003533"
     CDS             complement(12522..13301)
                     /gene="ygcR"
                     /locus_tag="ACHOSM_RS17665"
                     /old_locus_tag="pgaptmp_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HGU4559969.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRGRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(13279..14688)
                     /gene="ygcS"
                     /locus_tag="ACHOSM_RS17670"
                     /old_locus_tag="pgaptmp_003534"
     CDS             complement(13279..14688)
                     /gene="ygcS"
                     /locus_tag="ACHOSM_RS17670"
                     /old_locus_tag="pgaptmp_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4559970.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(14710..16164)
                     /gene="ygcU"
                     /locus_tag="ACHOSM_RS17675"
                     /old_locus_tag="pgaptmp_003535"
     CDS             complement(14710..16164)
                     /gene="ygcU"
                     /locus_tag="ACHOSM_RS17675"
                     /old_locus_tag="pgaptmp_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HGU4559971.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMTEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(16234..17019)
                     /gene="ygcW"
                     /locus_tag="ACHOSM_RS17680"
                     /old_locus_tag="pgaptmp_003536"
     CDS             complement(16234..17019)
                     /gene="ygcW"
                     /locus_tag="ACHOSM_RS17680"
                     /old_locus_tag="pgaptmp_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4559972.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIALGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
BASE COUNT         4152 a         4661 c         4219 g         4224 t
ORIGIN      
        1 ccctctttaa gcggcagacg aggtgttaaa ggatggcgcc acatgacgta ttgaacacca
       61 tagtttttga gtgacatcaa gcccaaaaga gcatcgctct gcctaccgca aatgtcgcag
      121 ttacctattg aggttgtctt aaaatcaata cgtatacgtc gtggcattcc ccagtaggcc
      181 tgaagtttat tgacttgttc atccgttacc acagcaccgt ccagttcgct ggtacgcgtc
      241 ggtgcaagcc aaggaaagac caggtcatca aaggtttttg gtaaaggtaa atcggcttca
      301 tcttgcggca ttacgtttag ccacagcttt cgccacaaag gggtttgttg attgccctga
      361 tactccagca attctataag tgtcgtcaga ggccctccgc cacgtagccc aggatagtaa
      421 ccgcgtccac ctataggtgc attcaactgg attgaaaaaa gcgctaaagg cacacaatgc
      481 aggcacagat gctgagttgt gtctcgctta ataaaatggt ctgtatttcg ttcttttgtt
      541 tgttttcctg gtgcgtcagg aagaagcgaa gcaatagaag ttgccttaac ttttaattca
      601 tcaaaatcct gcataaatga aggggaatct ggcccaaact ggaaagcatg ttctaatgac
      661 tgcaatgctt cccgtagctt ttcagcttcc agtccgtctt cccagatatc atcccaacga
      721 cgatgatctt ttggcgcgaa gctggtttgt agtagcccca gcaaaaactg ccataccgca
      781 ccctggagat ctgcccgcgg cgcagaaata tcgacaacat tttcatccgc cagatcgact
      841 ggcgccagtt tgcctgtcgt tccgtcttta aaacgaacgg gcaaccacgg ggttgtcaga
      901 agtgaaaacg agttcatacg catcctctcg taaaaaagac catcccaggt cggttgtcca
      961 tctttccatc cttttcggtg tatcaactat tcagagagtc ctgtcgcaga catttcaacc
     1021 aaccttgcca atcaatcctt ccgtagccga atacccgcaa ctttcgtcat cgttgactaa
     1081 aatcacgttt gccatctccg gatcttgtcg ctgttcaatg caccacagcc tgaacgcctc
     1141 tccctccagt aaagaaaact catcccgatg ttttttccac cagcttcggc gcactctgac
     1201 aacgctcatt tcccatgcgt gagcaccggt ggcataaggc ttcaccacac cgtcaataca
     1261 ggtagccagc cacagggaaa cagactcctc agccagacgt gtcgacagtt tttccgggag
     1321 gtaatcgttg atattggcgg catagccggg cctgaagttc agcacaaact ttttagccat
     1381 tgcgcgatca cagtaatatt tgcccacttg ctcctgctcg ctgcgggcaa atccttccgg
     1441 cattaccacg ccctcaccgt agacggattc aataagaagc cgggctgcgt gtggcatctg
     1501 aatagcacct tgctcacgca gcacacgctg cgtcagccag attcgcccat gatcgggata
     1561 gacaaatgca ctgttacgca tggcactgcc aaaccattcg tcgccaggag agtcgtccca
     1621 gacgggagcc agaatcagca attcaggggg ggaacgctcg tcctttccgt cacgctttaa
     1681 ctgaccatta atatcgcgga tatgccgctg taatcgtcct gctcgctgaa tcagcaaatc
     1741 aacaggggcc aggtcggaga tcatttcgtc caggtcaata tccacgcttt gctcaattac
     1801 ttgtgtacaa acaatgactt gcgacgctct ctgtaaacta caatatttac caaagcgtgc
     1861 cagcgtttcc gtttcaattc gctggcgatc gctaaaagca aagcggctat gaaaaagtga
     1921 aaggctggaa gcaggaatga cgccgcgggc aagcagctga cgatgaacct taatagcgtc
     1981 atcgacagaa ttccggatcc aggcgatgca tttcccctga cttaccgccg attcgatacg
     2041 cgcaatacac tcttgttcac tatgaagcca acccacgctg acgctacgct caacgtcttt
     2101 gcgcgtcgct acccggtgtg agttcacatc ggatttcgtg acatgcgtca gccaggggta
     2161 atcatccttt tccaggaacg gagcttcttg ctggccctct gtaccccgcg caaaggcagc
     2221 gacgagtttg tcgcgctgct gttgagataa cgtggcagaa agcaaaataa cgctgtttcc
     2281 gccacgcgcc tgccgctcga tcaacccttc aagaatgcac gacatgtaag cgtcacaggc
     2341 gtggatctca tcagccagca ggattttgtt actcagcccc agaagccgca gattattatg
     2401 tttgaacggc atcaccgcca tcatcgcctg atccagcgtg ccgacgccaa tttcagccag
     2461 caacgccttc ttgttactgt cggcaaacca ggccgcacat ccctgactga acgtttgttc
     2521 atccggttct tctgacccga ctaaatcacc agaccagagt gattcattaa agcggtccat
     2581 taatgtgcgg gcactatgtg ccagcaccag gctggggcgg gattctagcg aatagaaagc
     2641 aagccaggtt ttgaccagcc gatcgtacat ggcattggcc gttgccattg ttggcagacc
     2701 aaaaaacagc ccctgagctt tccccgcagc tatcaacctg tgcgccagga taagcgccgc
     2761 ttctgtttta cctgcgccag tcacatcttc cagaataaat aactgcggtc cttgctggct
     2821 gatatccaga tccagcacct tttgctgtaa cggtgtcgga ttctcaataa aaggaaacag
     2881 cgtattaatt ccgttgaaag gcgcgacttt tgcttttaga ggaaagacgg ttaaggcgtt
     2941 ttgagcctga actaaagttt tctgccagta acctttaata tccattgggt gtgcgacgcg
     3001 tggaaaaaat cgcgttgacg aacccgtcca gtctgcgagc acgacggttg cagagatata
     3061 ccaggaaagg tgttttataa gttcaatgcc ctcatcatca tcccagaatg cggggatctc
     3121 tatgagcgga aacagtggtt tgatttcaag gagaaaatct cgtgctgcag ctttgtcttc
     3181 aggcagaaaa ttatccaatt catccatacg atcagggggc cgaccatgat gccctgtcgt
     3241 cacgggcatc cacatttcta tcactcgcct aagtttgcgc ggagagagcg aagatgaagg
     3301 aagtaactcc tgacattcac ttaaataatg attccacaac cagtagccca gcgttgaatg
     3361 agagattttt tcgtaatttt ttctggcacc ttcctgaatc ttgagttcag ggggcaggta
     3421 aagttgttga aaagagcggg caaattttcc aatatcgtgc cagcacagca accaggcgaa
     3481 aaattgagcc gcctgttcct tgtcagaaat ccctaattga cgaaaataat cagccagccc
     3541 gaagcaattt cttttaacca ttaaatagcc catcgcggcc acatccagcg aatgccagca
     3601 aagaaggtga tagtcgtcgc ccccttcttt ctcgccgcgt cgggtttttc cccagaaatc
     3661 aaagaaagtc acaatatttt tatccttcag taaacttaaa ggatatttac gcatatttta
     3721 aaaattatct gtgatatata tcagccaata aaacatttac attatgaata aattaatgat
     3781 tttcatttga aattcataat aataaataga gactatatat tcacataaaa aatataaatt
     3841 ttaaaaaggg tgcaggagac caaaccatca caattcagta cgcggggttt tacccccgcg
     3901 tggtggctac ttcttcgatt cgtaagcgag aactgcctta aactccatac cacgctctct
     3961 gatactcaac tcacacaacg agtcgcctac catcagcgtg aatcccagta ctgtacaaca
     4021 cagtacgatg attgccacaa gggcatattt tgtcagcatg tcttgcctcc ctgcaaagaa
     4081 gagactaaca ttcgaattgt ttgggttcga acgtaagcct caggttgata gaaatatcgc
     4141 ctggggcttt tgtccatctg gaacctcgcg aatgcttaac gccagacagc ctcaagcacc
     4201 caacgccatt ctatacctga taattcttcc tgcgacgttc acctttaaat gccggaatgc
     4261 tgctcgcaaa ataaaaacgc ccccatcatt tctgacagag gcgtttaatt tatccggcaa
     4321 tatttaccct tcgtgtaacc cacattccct ttttaagcca aagaaacgcg tttcttcttc
     4381 cgccatgcca ggttcccatt tacgggttgt atgggtatcg cccaccgata aatatccttc
     4441 gtcccataat gggtgatatt tcaggccatg tttttgcagg tactgataaa tagttcggtt
     4501 atcccagtcg ataatcggca gcactttaaa tacgccacgc tgaattgcca gcaccggtaa
     4561 attagcgcga ctgccggatt gttcacggcg caggccagca aaccaggttt gcgcgttaag
     4621 ttctttcaga gcccggttca tcggttcgac tttgttgatg tcattgtact tttcaatgcc
     4681 ttcaacgccc tgctcccaca gttttccgta gcgtgcttcc tgccaggctg cgctttcggt
     4741 agcacggtac actttcaggt tgagcttgag tttgtccgtt aactcgtcaa taaagcggta
     4801 ggtttccggg aacaagtaac ccgtatcggt gaggatcacc ggaatatccg ggcgaatttg
     4861 attcaccaga tgcaggctca ccgccgcctg aatgccaaag ctggaagaaa gtacatattc
     4921 acctggcaga ttatccagcg cccaggctac gcggccttca gcgtccagtt tttccagttc
     4981 ggcgttagtt tccgccagcg ccagaatgcg atctaccttc ggcagttcgt tcagggcgtt
     5041 tagatcgagt ttggacataa attcctcact gtttgccttg cctgatgcga cgctcacgcg
     5101 ttcttatcag gcctacaaga ccgggctgat ggttaatccc acaaatcgcg cgccggatcg
     5161 agcaccgggc gaatgatgcc cgcacgcacc gtaaagtcgc cgaagccttc acccgcttcg
     5221 cgctctttcg cccagcgccc tatcagttca tcaagcgacg ccaggatttc cggctcggtg
     5281 atgttttctt tatacatccg tgggatacgt gtcccaatgc ggttgccgcc aagatgcagg
     5341 ttgtagcgac ccggcgcttt acccaccagg cccacttccg ccagcatcgc gcgaccacaa
     5401 ccgttcgggc agcctgttac acgcatcacg atatgctcat cgctgacacc atgtttcgcc
     5461 attaaattat cgatgttgtc gataaaagac ggcaggaaac gctctgcttc cgccatcgcc
     5521 agcgggcaag tcgggaatga cacgcaggcc atcgagtttt cacgctgcgg cgtgacggca
     5581 ttcattaacc cgctctcttt ggcgatcttc tcgatcttcg ctttctcgct ttccggtaca
     5641 ccggcgatga tcagattctg gttcgccgta atgcggaaat cgcctttgtg gatcttcgcg
     5701 atctccagca ggccggtttt cagcggacgc cccggataat caaggatgcg accattttcg
     5761 ataaacagcg tcaggtgcca gttatcatca atgcccttaa cccagccaat acgatcgcct
     5821 cgtccggtga actcatatgg acggatcggt tcaaatttga tccccgcgcg acgctccact
     5881 tccgctttaa acgtctcaac ccccacgcgc tccagcgtgt atttggtttt ggcattttta
     5941 cgatcggttc ggttacccca gtcacgctga gttgtcacga cggcttcggc caccgccagc
     6001 gtatgctcca gcggcagata gccaaactcg ctcgccgtgc gggcgtaggt tttcttgttg
     6061 ccgtgttcga tagaaagccc accgcccacc agcaggttga agcctaccag cttgccgttt
     6121 tcggcgattg ccacgaagtt catgtcgttg gcatgcagat cgatatcgtt ctgcggcggg
     6181 atcactaccg tggttttgaa cttacgcggc aggtaggtct gaccaaggat cggttcttca
     6241 tcagtagtgg cgactttttc ctggtcgagc cagatctccg catacgcgcg ggtacgaggc
     6301 aacagatgct cagaaatctt cttcgcccac tcgtacgctt ccgcgtgcag ctgcgactcg
     6361 taagggttcg aggtgcagag tacgttacgg ttcatgtcgt tagctgtcgc cagcgcatca
     6421 agaccgaccg agtgcagcat ctggtgcacc ggtttgacgt tctttttcag aatgccgtgg
     6481 aactggaatg tctggcggtt ggtcaggcga atgctgccat agatggtgtt ttcaccggca
     6541 aatttgtcga tcgcctgcca ctgtttagtg gtaatcaccc cacccggcag acgacagcga
     6601 agcagcatcg cgtggcgcgg ctccagcttc tgttcagcac gttcggcgcg gatgtcgcgg
     6661 tcatcctgct gatacatgcc gtggaagcgg atcagcagga agttgtcgcc cttaaagccg
     6721 ccggtcagac cgtcgtttaa atcttccgca atggtgccgc gcaggtagtt gctttcaagc
     6781 ttcatgcgct cggcgtctgt cagttttcct tcgaccacta aaggccctgg atgtttttcg
     6841 ctcattagta gacatctcgc tgataacggc gctctacgcg cagctcactt aaaaattcat
     6901 ccgccgcttc ggtgtccatg ccaccaaatt cggcaatcac ttccagaagt gcctgctcaa
     6961 cgtctttcgc catgcgatta gcgtcgccgc agacataaat gtgggcacca tcattgatcc
     7021 agcgccacag ctccgcgccc tgttcgcgca gtttgtcttg tacgtaaact ttttcttttt
     7081 ggtcgcgcga ccaggcaaga tcgatacgtg tcagcacgcc atctttgacg tagcgctgcc
     7141 actccacctg gtacaggaag tcttccgtaa agtgcggatt accaaagaac agccagtttt
     7201 tacctggcgc ttcgtcggcg gcgcgttgct gcataaaggc gcggaacggc gcaataccgg
     7261 tgcctgggcc aatcataatc accggggttt ccgggttagc gggcaggcga aagttatcgt
     7321 tatgttcgat aaatacgcgg acttcgccct cttcttccac gcgatccgcg aggaagctgg
     7381 aggcaccacc ggcacgggct cggccttcca cgtcgtaacg caccacacca acggtgacgt
     7441 gtacttcgtt ctcgacttcc gcctgcgagg aggcgatgga atacaggcgc ggggtcagcg
     7501 ggcgcagcag attaatcagc gcttcggcat ccagttgcgc cggagagaaa cgcaccatgt
     7561 cgacaatcgg cgtcgtcgcg gcgtaatgct gtaacttcgc tttatcgccc accagcggca
     7621 gcagtgtttc gctgcgggta agcgtggcgt aattctcaac aatgttggcg gtgttgacgg
     7681 tcagttcgaa gtgccactgt agcgcttcgt tcagaggcaa cgttttgccc tcgacggtga
     7741 caggttcatc gcctttcagc cacagcagtt cgacaagttc tttcaccagt gccggatcgt
     7801 tctgatacca gacgcccagc gcgtcacccg gctggtaacg caggcccgag tcacctaagt
     7861 caatttcgat atggcgaacg tctttttcag agttacgccc ggtaattttc tggttaacag
     7921 agaggctagc taccagcggc gcgtctttgc tgtacgggct ggtgtggatt tcatttaccg
     7981 cgccagtagc gacggattgc gaaggtgccg cgacaggcgc acgcgattta agcgcatcaa
     8041 ccacgcgggc gcgccactcg ctggcagcag ccaggtattc aacatcggca tcgacacggt
     8101 cgagcaggcg ttcaccaccc agttccgcca gcttgctgtc gaaatctttc ccggactggc
     8161 agaaaaattc ataagagcta tcgccgaggc taaacacggc aaacgcggtg ttttccagct
     8221 ttggcgcttt tttggagaac aggaacttat gcagcgcgac ggcttcttcc ggcggttccc
     8281 cttccccttg cgttgacgtc actacgatga gcagtttttc gctggcgatt tgtttgaatt
     8341 tatagtcgcc cgcgttcacc agcttaacgt tcagttttgc tgctaataaa tcatcgcgta
     8401 atgcttcagc aacccggcgc gcattgccgg tttgcgaggc ggagataata gttatacccg
     8461 gcatttctgc ggctggcgct ggcgtcgctg caagcgcagc aggctgctga ttgagtacgc
     8521 cccagaaata gccagaaacc caggcaagct gggtaggcgt taaatcggtc gtggccgcct
     8581 gaaggcgtgc cagttgctcc gggttcaacg gaagcaacgc ggaaggtggg acctgtgtcg
     8641 tcatgcgtcg ttatgttcca gtaagcaaag ctgtttctgc gccctgtcag cgcccataaa
     8701 acagaagaga agataaggtt aacggggtga acggtgtgga ttaaagacgg gatagcgata
     8761 actaataacc aaatcgacta acctgtttta gcaatagtct ttaacaacaa aatagattaa
     8821 ccaacccaat gaaaaacaaa tgaatttagc caatcattaa gataaatcag cgattttgcg
     8881 caacaaagtc gttttagata atgcgaaaaa acagcctttc cggtactcta cggcggtttc
     8941 atcgtcctgt agagaaatta tgatgtccac cacgttattt aaagatttca ccttcgaagc
     9001 cgctcaccgc ttaccacacg tcccggaagg gcataaatgt ggtcgcctgc acgggcattc
     9061 ctttatggtg cgactggaaa ttaccgggga agtcgatccg catacgggct ggattatcga
     9121 tttcgctgaa ctaaaagcgg cgtttaagcc aacttatgag cgcctcgatc accattatct
     9181 caatgatatt ccaggtctgg aaaacccaac tagcgaagtt ttagcaaaat ggatttggga
     9241 tcaggttaaa cccgttgtgc cgctgttaag tgcggtgatg gtaaaagaaa cctgcaccgc
     9301 tggttgtatc tatcgcggcg aatgataaga gtgtgtcggc ggtcaatttc ccttaagtaa
     9361 cgctatgtta gggtgttgtg ttctggatat ctggggcatg acatggaaga cgactgcgac
     9421 attattatta ttggtgccgg tattgcaggc accgcttgcg cgttacgctg cgcgcgagcg
     9481 ggtttatccg ttttgttact ggaacacgct gaaatccccg gcagcaaaaa tctttccggc
     9541 gggcggttat atacccatgc actcgcggaa ctcctcccgc aatttcatct gaccgcgcct
     9601 cttgaacgac gcatcactca cgaaagcctt tccctgttaa cgccggatgg cgcaacgacg
     9661 ttttccagct tacagcccgg cggtgaatcc tggagtgtat tacgtgcacg gttcgatccg
     9721 tggctggttg ccgaagccga agaagaaggt gtcgaatgca tccccggagc gacggtggat
     9781 gcactgtatg aagaaaacgg cagggtgtgt ggtgtcatta gtggtgacga tattctccgt
     9841 gcccgttatg tggtgctggc agaaggtgcc aacagcgtcc tggctgagcg tcacgggtta
     9901 gtgactcgtc ctgctggtga agcgatggcg ttggggatca aagaagtgct gtcgctggaa
     9961 ccatccgcta ttgaagaacg ttttcatctg gagaataacg aaggcgcagc gttgctgttc
    10021 agcggcagga tctgtgatga cttacccggc ggcgcatttc tttatactaa tcaacaaacg
    10081 ctctcgttag ggattgtttg cccgctctct tcccttacgc aaagtcgtgt tccggcaagc
    10141 gagctgctga ctcgctttaa agcgcatccg gcagtgcgcc cgcttatcaa aaacacggaa
    10201 tcactggagt atggtgcgca tctggtgcca gaaggtggct tgcacagtat gccggtgcaa
    10261 tacgccggta acggctggct gctggtgggc gatgcgttgc gcagttgcgt caataccgga
    10321 atttccgtgc gcggcatgga tatggcgctg actggcgcgc aggcggcggc acaaacgctg
    10381 ataagcgcct gccagcaccg cgagccgcaa aatctgtttg cgctttatca tcacaacgtc
    10441 gagcgcagcc tgctgtggga tattctacag cgttatcagc atgttccggc gcttttgcaa
    10501 cgccctggct ggtatcgggc gtggcctgcg ttaatgcagg atatttcccg cgatttatgg
    10561 gatcagggtg ataaacctgt tccaccgctg cgccagttat tctggcgtca tttacgtcgt
    10621 catggcctgt ggcatctggc gggcgatgtt atcaggagtc tgcgatgtct gtagcccata
    10681 atctctggcg cgttgctgat gcgccgcaca ttgttccggc tgactccgtt gagcgtcaga
    10741 tggtacaacg gttgattaac gcctgtccgg caggtctttt ttcgctcaca ccggaaggtg
    10801 acttacgcgt tgattatcac ggctgcctgg agtgtggcac ctgccgtttg ctgtgcgacg
    10861 aatccacact acaacagtgg cgctatccgc cttccggatt cggcatcacc taccgctttg
    10921 gataaataat aaggataatt tatgcccctc ttacacctgc tccgccagaa tccggtaatt
    10981 gctgccgtta aagataatgc cagcctgcaa ctggctatcg attctgaatg ccagtttatt
    11041 tccgtgttgt acggcaatat ctgcaccatc agtaatatcg ttaaaaagat taagaacgcc
    11101 gggaaatatg cttttattca tgttgatttg ctggaaggcg cgtcaaataa agaagtcgtt
    11161 attcagtttt tgaaactggt gaccgaggcg gacggcatta tcagtaccaa agcctcaatg
    11221 ttgaaagcgg caagagcaga aggttttttc tgtattcatc gcctgtttat tgttgattca
    11281 atttcgtttc acaacattga taagcaagtt gcgcaatcga atccggattg tattgagatc
    11341 ctgccaggct gtatgcccaa agtgctgggc tgggtgacag agaaaatccg ccaaccgctg
    11401 attgccggtg ggctggtgtg cgatgaagaa gatgcgcgta atgcgattaa cgcgggtgtc
    11461 gtggcgcttt ccaccacgaa taccggggtc tggacgttag cgaaaaaatt actttgacgg
    11521 gataatcgta accaattgaa tttggtttga tttgtaggcc gcacgccaca tccgacattc
    11581 agcgcctgat gcgacgcttg acgcgtctta tcaggcctac aagtccccag gccagaaccg
    11641 taggcaggat catccggcaa cacgttattg gcaatctgcg tgaatgtttg tcaccagtgc
    11701 ctccagcacc actttccagt catcaaccac gccaacatct gcctgtgaaa ataccgcagc
    11761 gctggcgtca tgattaatcg ccaccacaaa cttgctattg cgtacgcccg ccattaacgc
    11821 tgctgcgcca gaagcgccaa caacaataca cacctcaggt gccagcagat gtccggaaat
    11881 accaatcact ttttcagcat caacgccacc gttcatcact cgcgcccgac tgtagcccac
    11941 ttcggctccc agcttttctg ccagcatagc gatttcctgg ttatctgcct ctccgccctg
    12001 ccccaccacc agcacccgtc tggcctcagc taatgggtcg cgagtaacat ttttcagatc
    12061 ctctgtgcta accagccagt ccggaagcgc acccgggaca atgttcagtt gttgcattcc
    12121 agagggcaac gtggcgtttt tcgctgctcc cgcctgacgc gccagggaaa ggcatagcgg
    12181 gcgtttttct gtttgcagcg tcgccgttag cgcatttccc cagtgggatt tgcgcacgct
    12241 tacggttgga atatctaacg aagtcacctg gcagatgctt gcaccatgta aacgccaggc
    12301 cagccgggtc gacagttcat cgccaaacgt tcccggcgga aagagaacaa cgtccgcagg
    12361 cgttcgctgc cactgttcca ccaacgcatc cagcacctgt tccgccacta caggctgagg
    12421 ttcaatctgc cagtgcgcca gggtacaacc gctgaaatcc tgcgccgcca gccagcttgc
    12481 tattgcggca ttctcctgat ttattgtcac aattgcgata ttcatggctg catcctttgg
    12541 cgtaaatagt cctgccatag cttttgagct ttttctgcta ccgtttgccc atcaatcagc
    12601 gtcgctccgc gtcgttgctc tgctcttgcc agttgcagac actgcatcgc tggcatttcg
    12661 gcagcaacag ttttacggat aatttctgct ttccccgcag ccattcgctg gcgcattccc
    12721 ggtactggca aggccacttc accgcactga cgcacggcaa tcaccgcagg caagcgaccc
    12781 cggcaacaac gcaacccatg ttcagtacgc tgttcgaggg tgataaacag cgcgtcgaga
    12841 gtgaaacgct ccacctgggt gaagcagggc cagcccagca tttccgccag caaaaatggc
    12901 gtttgcccat tctgcccttc gctgctttgg cagccggtaa ttatcagatc cagcggattc
    12961 tgatgctgcc attcggcaat atggcgagcg acaaattccg gcgcaaagcg cagatctgcc
    13021 gccgtctcca gcaatacagc ttcctcaaac ccaagagcca tgagatagcg cagccagtgc
    13081 aatgcccgtt catcccccat gctcaacgcg gttaaagaca tcggcgtacc gttttttctc
    13141 tgcgccaaca gcagcgcggc agcggcctgt tcatcagcac cgagtaaact tcgcagtagc
    13201 gaaatatccg gtccgctttt accctgagcc gccgcctgcc actctttttc cgccagcatt
    13261 ccggcatccg gttcggcttt aaacgctaac agaatgttca ttcgcacctc ctacatttcc
    13321 cgccgccacc agcgggaggg ctttagtttc tggtgcccat agccaggtca caacaaaacc
    13381 aaccaacaac actgtcgcca ggagcaataa ggtgacttgc attccccact gcgccagcac
    13441 ccacggcagc aggccagtac ttaccgccgc gccaagtcga ctcatggcgg tggcaaaacc
    13501 gacgcccagc gagcgaatgt cagtaggaaa actttccgca ggcaaaatgc ccaccagatt
    13561 actgactgcc gaaatggtgg tgctgaagag aacaaaaagc agcagcgtta atgaactgcc
    13621 ggaaggcaaa caggccatta ctaccagcgt tgccgccagc agcaaaaaac ttcccagcaa
    13681 aaatttgcga tgtgccagca ggtgcgtcag aactaatccc agcagcgcgc ccacaattaa
    13741 caacgcatta agcatcaggc tggcagtcag cgcatcttcc agaccaatag tctgggcgat
    13801 agttggcagc caggtataaa tcacaaacca tgggattacg aggcagacaa agaagacgct
    13861 gttaaacgcc gtgcgccgcc agtaacgcga agagaacaag gttttgatgt gtttatgggt
    13921 cgccgttacc acttcatcgc ccagtaaaac atggggacca aaatagcgat gcacgatagc
    13981 gtgagcttct gcaaaacgcc cctggcgcag tagccagcgt ggtgattctg gcgttcccca
    14041 gcgtaataac gtaatcaaca acgcgggcag agctgccgat gccagtagcc agcgccaggc
    14101 ctccgggttt tcggaaataa agtgatgtcc ggcaatactt gccagcacat agcctacggt
    14161 ccacaccacg ctgaatgcgc ccagcaaaat accgcgatgg cggcgcgggg aaaattcagc
    14221 cagcaaggtg tgacctactg aataatcgcc tcccagacca atgccaatca aaatgcgcag
    14281 tccaataaga tgctctggcg tggtggcaaa aaattgtaaa aacgaagcaa gcgtaatcag
    14341 caaaaagctg aaggtgaaga ttttttgccg accaatatgg tcggagatcc acccaagaac
    14401 caggctgcca aggaacaaac caaggagcgc cgagccgccg atcattcccg ccataaacgg
    14461 cgtcagttgc atggcgggcg taagctgaat aatggcgtaa ccaatgacgc cgagaacata
    14521 accgtcggtc aggtgtgcgc cgaaagtgag cgcagcaatg cggcagtgaa aacggttaag
    14581 cggtaaatca tccattcgca ccggtgaagt gttcatcttt accccttcat tgtccggtaa
    14641 taagaacggc ttctcgcctg agaagccgaa aaggcaacgc cccgtcatcg cggaacgctg
    14701 cctgacacat tatttttcaa tcggatagat agtacccgta ttcataatgc cattaggatc
    14761 gaactgcttt ttcagccctt ccagcaacgc ccacgcgctg ccgtgttcca gtttgctcca
    14821 gtgaacgcga tgtttaccga taccgtggtg gtgcaccatc gaaccgccga ggcgaatggt
    14881 ttcttcacag atgatcttgt tgagcggatt gtggtacttg tcgatttcct cttccggctt
    14941 acagtcaacg acgttgtaat cgtagacgaa gtacatgttg gtgccgttct gatagctatg
    15001 agaggaatga ccgcccagca tggtgatgtc gtcggcgtgc gggaactcag tacgaatacg
    15061 gttaataacg ctttcgtaga tttcgtggat gcagctccag cagccggaca cttcagtggt
    15121 aaagcccatg ttgccggttt tgaggatctg cacacgttcg gcagccactt tatccggtcc
    15181 ccagttcagg ttgttaaacc aggtttcgat cagcttgctg tccacgcgct ggcattgcgg
    15241 gtagcgggca acgatttccg caatcccttc gcccgtcgcc ttcgcaatgc gagggttacc
    15301 ttcagtcata aagatcagca cgcattttcc gtctgcaaaa tgggtgaagt gttgggtgcc
    15361 atcttcagcg tcatacaaac gagcgatcga cggacgatac ccttccacca tgacttcacg
    15421 caggatgttg aagccggttt tcatgtcttc caggatatag ccgtagaaga ggttgttttc
    15481 cggggtgaat ttaaagattt tcactgttac ttcagtgata tagcaaagtg caccttcgtt
    15541 accgatgatg atgtggcgaa tgtccgggcc agccgcgcgg cgtggcacgt ttttaatgcg
    15601 tgtgacggtg ccatcagcca atactgcttc cagaccaacg accatatctt cgattgcgcc
    15661 gtagagtgtg gagaactgcc cgatactgcg ggttgctacc aggccgccca tctgcgccag
    15721 cggctttgac tgcggagaat gccccgtggt gtaacctttt tcacgcaacg cattttccag
    15781 cacctccagc ggaacgccac attgcgccgt cgcctgcata ttctcaatat caatattaat
    15841 gatttgattc atggcggagc cgtcgagcac caccgagttt tccacaacgg tttccagccc
    15901 accttcagtg gcggaagcgc cggtacgcgg cacaccgtta attttgtgcg cattcataaa
    15961 attcagcaca cgggatactt gctctgtgga accgagtttt acgaccgctg ccggaatcgg
    16021 caaagtataa atgccatgaa tatccggaaa tttacgaaaa cggtcaatac tgttcttctt
    16081 taatactgtt tcatcggtaa ttacgcgatc tgcaccaaca atttccttta gttggtcgac
    16141 aatcgctgcg cgagataaag acatagtaat tccttcctga taataaaaat ggaataatca
    16201 ttaataagag aaaacagaaa ccaccgttat ggattagcgc actaaataac caccatcgac
    16261 cactaataaa tgcccattga cataatttga agccggactt gcgaggaata cagctgcgcc
    16321 cattaaatcc tgagtatcgc cccaacggtt tgccggaata tgatcaagaa cgcgctgatt
    16381 ggtttctgga ttactgcgtg tcgccagcgt aatatctgtt gcataatagc caagggcaat
    16441 accatttacc tgaatattat attgacctag ttcatcacaa taagctttgg tgaatccggc
    16501 aagagcatgt ttagtggcag aatatgcagg tgaccattgt ccacctaagt aagagaacaa
    16561 tgaacagata ttaataattt tgccgctttt ctgcgggatc ataatttttg cagcttcata
    16621 gcttaactcg aatgcggcgg tcaggttcac atcaatcatc ggatcccagt cggcacgacc
    16681 gaagtccagc accttattca gcttacaaat accggcattg tttaccagaa tatcaactgt
    16741 accgaaacgc tcacagcaag cagcgataat cttctgcggc gcgccttctg cggtgatatc
    16801 cacctgcatg aagtccacct caacaccctg tttttcaatc atttcctttg tttcgccgtt
    16861 atctttgacg aaactaggaa taaagatatt tgcgccagct ttggccaacg ccatggcaaa
    16921 tgcctggcct aaaccgctat tcccaccggt aacaattgcg gttttacctt tcagtgagaa
    16981 aaaatccatt gagaacgcat tgagagattc gattgacata gttagctcca atttccgtaa
    17041 ggcaaaaaaa aagaaaggta acttcccccg cgagagggaa gttacctttc tcattatctc
    17101 tggtaactgg agataatttt tagcataagg acataactgc taatgtgatc gtaatcacag
    17161 tgtgatattc gtattgtcaa ataattttaa aaagcagaag cgtgattact ctcacacaac
    17221 acctctgctt tccatgttat ttattatttc aattac
//
LOCUS       DAZKHC020000087        16974 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZKHC020000087 DAZKHC020000000
VERSION     DAZKHC020000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.087"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..333
                     /locus_tag="ACHOSM_RS17685"
                     /old_locus_tag="pgaptmp_003537"
     CDS             <1..333
                     /locus_tag="ACHOSM_RS17685"
                     /old_locus_tag="pgaptmp_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF550 domain-containing protein"
                     /protein_id="HGU4559973.1"
                     /translation="AINHHLLMVSTVCAEAYQVVGVMADALGVFGDAAVQKVLDNLSQ
                     QKLVHRDVLPFSLPVTPDGWVMVPKQVTPEISNAINVVGQRCTCGNCSQRLWDLLLDA
                     TQQGVNRG"
     gene            434..778
                     /locus_tag="ACHOSM_RS17690"
                     /old_locus_tag="pgaptmp_003538"
     CDS             434..778
                     /locus_tag="ACHOSM_RS17690"
                     /old_locus_tag="pgaptmp_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4559974.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEKIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            885..1103
                     /locus_tag="ACHOSM_RS17695"
                     /old_locus_tag="pgaptmp_003539"
     CDS             885..1103
                     /locus_tag="ACHOSM_RS17695"
                     /old_locus_tag="pgaptmp_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4559975.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            1081..2154
                     /locus_tag="ACHOSM_RS17700"
                     /old_locus_tag="pgaptmp_003540"
     CDS             1081..2154
                     /locus_tag="ACHOSM_RS17700"
                     /old_locus_tag="pgaptmp_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4559976.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELLSGSRRESLIDRIKGADAITLHAWLGRYETILSERGIRLKTLLDYAS
                     KIRAIRRKLPDKPLADISTKEVAAMLNTYVAEGKTASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYVAIHHAAEPLPIWLRLAMDLAVVTGQRVGDLCK
                     MKWSDINDNHLHIEQGKTGAKLAIPLTLTIDALNISLADILQKCREASGSDTIIASTH
                     HEPLSPKTVSKYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDSMAARYRDSRGREWDKIEINK"
     gene            2498..3745
                     /gene="mdtA"
                     /locus_tag="ACHOSM_RS17705"
                     /old_locus_tag="pgaptmp_003541"
     CDS             2498..3745
                     /gene="mdtA"
                     /locus_tag="ACHOSM_RS17705"
                     /old_locus_tag="pgaptmp_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HGU4559977.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITSPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPSAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            3745..6867
                     /gene="mdtB"
                     /locus_tag="ACHOSM_RS17710"
                     /old_locus_tag="pgaptmp_003542"
     CDS             3745..6867
                     /gene="mdtB"
                     /locus_tag="ACHOSM_RS17710"
                     /old_locus_tag="pgaptmp_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HGU4559978.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     RQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            6868..9945
                     /gene="mdtC"
                     /locus_tag="ACHOSM_RS17715"
                     /old_locus_tag="pgaptmp_003543"
     CDS             6868..9945
                     /gene="mdtC"
                     /locus_tag="ACHOSM_RS17715"
                     /old_locus_tag="pgaptmp_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HGU4559979.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRIGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHPRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLWLRFSRKPKQTVTE"
     gene            9946..11361
                     /gene="mdtD"
                     /locus_tag="ACHOSM_RS17720"
                     /old_locus_tag="pgaptmp_003544"
     CDS             9946..11361
                     /gene="mdtD"
                     /locus_tag="ACHOSM_RS17720"
                     /old_locus_tag="pgaptmp_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4559980.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            11358..12761
                     /gene="baeS"
                     /locus_tag="ACHOSM_RS17725"
                     /old_locus_tag="pgaptmp_003545"
     CDS             11358..12761
                     /gene="baeS"
                     /locus_tag="ACHOSM_RS17725"
                     /old_locus_tag="pgaptmp_003545"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HGU4559981.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHTLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            12758..13480
                     /gene="baeR"
                     /locus_tag="ACHOSM_RS17730"
                     /old_locus_tag="pgaptmp_003546"
     CDS             12758..13480
                     /gene="baeR"
                     /locus_tag="ACHOSM_RS17730"
                     /old_locus_tag="pgaptmp_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HGU4559982.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            13671..14003
                     /gene="yegP"
                     /locus_tag="ACHOSM_RS17735"
                     /old_locus_tag="pgaptmp_003547"
     CDS             13671..14003
                     /gene="yegP"
                     /locus_tag="ACHOSM_RS17735"
                     /old_locus_tag="pgaptmp_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HGU4559983.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            14212..14508
                     /locus_tag="ACHOSM_RS17740"
                     /old_locus_tag="pgaptmp_003548"
     CDS             14212..14508
                     /locus_tag="ACHOSM_RS17740"
                     /old_locus_tag="pgaptmp_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU4559984.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            14510..14806
                     /locus_tag="ACHOSM_RS17745"
                     /old_locus_tag="pgaptmp_003549"
     CDS             14510..14806
                     /locus_tag="ACHOSM_RS17745"
                     /old_locus_tag="pgaptmp_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4559985.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            14909..16270
                     /gene="yegQ"
                     /locus_tag="ACHOSM_RS17750"
                     /old_locus_tag="pgaptmp_003550"
     CDS             14909..16270
                     /gene="yegQ"
                     /locus_tag="ACHOSM_RS17750"
                     /old_locus_tag="pgaptmp_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HGU4559986.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(16601..16918)
                     /gene="yegR"
                     /locus_tag="ACHOSM_RS17755"
                     /old_locus_tag="pgaptmp_003551"
     CDS             complement(16601..16918)
                     /gene="yegR"
                     /locus_tag="ACHOSM_RS17755"
                     /old_locus_tag="pgaptmp_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HGU4559987.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
BASE COUNT         3966 a         4447 c         4533 g         4028 t
ORIGIN      
        1 gccatcaatc atcatttgct catggtttcc acggtatgcg ctgaggctta tcaggttgta
       61 ggagttatgg cagatgcgct tggtgtattc ggtgatgcag cagtacagaa agttctggat
      121 aacctgtcac agcaaaaact tgttcacaga gatgtgctgc cgttctcgct tccggtaact
      181 ccggatggtt gggttatggt tcctaaacag gtaacgccgg aaatcagcaa cgcgataaac
      241 gttgtgggtc aacgctgtac atgcggaaat tgctctcagc ggttgtggga tttattactc
      301 gacgccacac agcaaggagt taaccgtggc taacctgcaa cttgccgtca aaggtgaata
      361 acaatcctcg cactcgcggg gatttctttt atctgaactc gctacggcga gttttgtttt
      421 atggagacaa gaaatgtcag atttggctat gaaggttttg aaatggcaat cgactggcga
      481 tgtcggcatc agtagtgcaa ctcttgcctc aatcgcatgt ggactgaaaa agaatatcta
      541 tggtcatcac ttcggcgctc cccatgacgc agcagacttc cgacgatgcg ttgctcttgt
      601 tgagaagatt ccagaaatca gagattcatt cgacaaggtt gcaaagcgcg ttccggcatt
      661 caaaggcatc ctcaacgaat gggattccct cgttgctctg ttgaagtctg aaatgaagat
      721 acacggaaac aaagcaccag agacttacag gagaatcagc gagttacgaa aggactaacc
      781 acagcctcac actcgatgag gcctgttcat ggctcaatga tatccagacc taccatcgcc
      841 gcatcaatgc ggcttttctt gcgtgtaatt gcggagactt tgcgatgtac ttgacacttc
      901 aggagtggaa tgcacgccag cgacgcccaa gaagccttga aacagttcgt cgatgggtgc
      961 gcgaatgcag gatattccct cctccggtta aggatggaag agagtatctg ttccacgaat
     1021 cagcggtaaa ggttgactta aatcgaccag taacaggtag ccttttgaag aggatcagaa
     1081 atgggaagaa ggcgaagtca tgagcgccgg gatttacccc ctaaccttta tataagaaac
     1141 aatggatatt actgctacag ggacccaagg acgggtaaag agttcggatt aggccgagac
     1201 aggaggatag caatcactga agcaatacag gccaatattg agttgctatc cgggagcagg
     1261 cgtgagtcac tgatagacag aattaaaggc gctgacgcaa tcactcttca tgcgtggctt
     1321 ggccgatatg aaacaatcct cagcgagagg ggtatcaggc tgaaaactct actcgactac
     1381 gccagcaaaa tcagggcaat ccgaagaaaa ttgccggaca aaccgctcgc tgacatatca
     1441 acgaaagagg tggcagcaat gctaaacacc tacgtagcag aaggtaaaac ggcttccgca
     1501 aaattaatca ggtcaaccct tgttgacgtt tttcgtgagg caatagccga ggggcatgtg
     1561 gctacgaatc cggtaacagc aacccgcaca gcaaagtcag aagtaaggcg ctcaaggctg
     1621 acagctaatg agtatgtcgc gattcaccat gcagccgaac ctctcccaat ctggctgagg
     1681 cttgcgatgg atttggccgt cgttacaggt cagagagtcg gcgatttgtg caaaatgaaa
     1741 tggtcagaca taaacgacaa ccatcttcac attgaacagg gtaaaacagg ggctaagctc
     1801 gccattccgc tgacgctaac gattgacgcg ctcaatatct cattggctga tatactacag
     1861 aaatgcaggg aggccagcgg cagtgacact ataattgcat caacgcatca cgaaccactt
     1921 tccccgaaaa cagtatcgaa gtattttaca aaggcgagaa atgcatctgg actctcattt
     1981 gatggagacc cgccaacatt ccatgaactg cgtagcctgt cggcgagact ataccgcaat
     2041 cagattggcg ataagtttgc tcaacgtctt ctcggacata aatcagattc aatggcggcg
     2101 cggtataggg acagccgagg gagagaatgg gacaaaattg aaatcaacaa atgattttat
     2161 tttgactaat aatgacctgc tcatggtaat ttgttgataa ttaaaggaat tttaaatatg
     2221 gaacttacct tatttaaggc cccttcctcg ggaggggctt tcccgtttca gcatccggct
     2281 gaaatctgcg gcttacctcc tttcgccctg tatgcagtct atcgctaacg cgtagataaa
     2341 atagtttcct gtgttattac tggatgcgtg ctcgcaaatg tgcccgccat tcagacgatt
     2401 cccgatagtg tttcataatt cctccatttt tctcccttat tggctggcta cactagtatc
     2461 attccgcgaa acgtttcagg aagagaaact cttaacgatg aaaggcagtt ataaatcccg
     2521 ttgggtaatc gtaatcgtgg tggttatcgc cgccatcgcc gcattctggt tctggcaagg
     2581 ccgcaatgac tcccggagtg cagccccagg ggcgacgaaa caagcgcagc aatcgccagc
     2641 gggtggtcgc cgtggtatgc gttccggccc attagccccg gttcaggcgg cgaccgccgt
     2701 agaacaggca gttccgcgtt acctcaccgg gcttggcacc attaccgccg ctaatactgt
     2761 tacggtgcgc agccgcgtgg acggtcaact gatggcgtta catttccagg aaggccagca
     2821 ggtcaaagca ggcgatttac tggcagaaat tgaccccagc cagttcaaag ttgcattagc
     2881 acaagcccag ggccaactgg caaaagataa agccacgctt gctaacgccc gccgtgacct
     2941 ggcgcgttat cagcaactgg caaaaaccaa tctcgtatcc cgtcaggagc tggatgccca
     3001 acaggcgctg gtcagtgaaa ccgaaggcac cattaaggct gatgaagcaa gcgtcgccag
     3061 cgcgcagctg caactcgact ggagccgcat cacctcacca gtcgatggtc gcgttggtct
     3121 caagcaggtt gatgttggta accaaatctc cagtggtgat accaccggga tcgtggtgat
     3181 cacccagacg catcctatcg atttgctctt taccctgccg gaaagcgata tcgctaccgt
     3241 cgtgcaggcg cagaaagccg gaaaaccgct ggtggtagaa gcctgggatc gcaccaactc
     3301 gaagaaatta agtgaaggca cgctgttaag cctcgataac caaatcgatg ccacaaccgg
     3361 tacgattaaa gtgaaagcac gctttaataa tcaggatgat gcgctgtttc ccaatcagtt
     3421 tgttaacgcg cgcatgttag tcgacaccga acaaaacgcc gtagtgatcc catcagccgc
     3481 cctgcaaatg ggcaatgaag gccattttgt ctgggtgctg aatagtgaaa acaaggtcag
     3541 caaacatctg gtgacgccgg gcattcagga cagtcagaaa gtggtgatcc gtgcaggtat
     3601 ttctgcgggc gatcgcgtgg tgacagacgg cattgatcgc ctgaccgaag gggcgaaagt
     3661 ggaagtggtg gaagcccaga gcgccaccac tccggaagag aaagccacca gccgcgaata
     3721 cgcgaaaaaa ggagcacgct cctgatgcag gtgttacccc cgagcagcac aggcggcccg
     3781 tcgcgcctgt ttattatgcg tcctgtcgcc accacgctgc tgatggtggc gatcttactc
     3841 gccgggatta tcggttatcg cgccctgccc gtttcggcgc tgccggaagt ggactatccg
     3901 accattcagg tggtcacgct ctacccaggt gccagcccgg atgtcatgac ctctgccgtt
     3961 accgcgccgc tcgaacgcca gttcgggcag atgtctggcc tgaaacagat gtcgtcgcaa
     4021 agttccggcg gtgcgtcagt tatcactttg cagttccagc taacattacc gctcgatgtc
     4081 gccgagcagg aagtgcaggc cgcgattaac gctgcgacca acttgttgcc gagcgatctg
     4141 cctaacccgc cggtttacag caaagtgaac ccggcagatc cgccgatcat gacgctcgcc
     4201 gtcacctcaa ccgccatgcc gatgaggcaa gtggaagata tggtggaaac ccgcgtcgcg
     4261 cagaaaatct cgcagatttc cggcgtcggc ctggtgacgc tttccggcgg tcagcgtccg
     4321 gctgttcgcg tcaaacttaa cgctcaggcg attgccgccc tcggcctgac cagcgaaacc
     4381 gtgcgcaccg ccattaccgg cgctaacgtt aactcggcaa aaggtagcct cgacggccct
     4441 tcccgtgcgg tcacgctttc cgcgaacgac cagatgcaat ccgccgaaga gtatcgccag
     4501 ctaatcatcg cctaccagaa cggcgcgcca attcgtctgg gcgatgtcgc aactgtagag
     4561 caaggtgcag aaaacagctg gctcggcgcg tgggcgaaca aagaacaggc cattgtgatg
     4621 aatgttcagc gccagcccgg tgctaacatt atctccaccg ccgacagcat tcggcagatg
     4681 ctgccacagc tcactgagag tctgccgaaa tcggtgaagg tgacagtgct ttccgatcgc
     4741 accaccaata tccgcgcatc cgtcgatgat actcagtttg aattgatgat ggctatcgcg
     4801 ctggtagtca tgattatcta cctgtttttg cgcaatattc cggcgaccat cattcccggt
     4861 gttgctgtac cgctgtcgtt aatcggcact ttcgcggtta tggtgtttct cgatttttca
     4921 atcaataacc tgacactgat ggcgttaact atcgccaccg gattcgtggt cgatgacgcc
     4981 atcgtggtga tcgaaaacat ttcccgctat atcgaaaaag gcgaaaaacc gttggcggcg
     5041 gcgctcaagg gcgcaggtga aatcggcttt accattatct cgctgacctt ctcactgatt
     5101 gcggtgttga tcccactgct gtttatgggc gatatcgtcg ggcgactgtt ccgcgaattt
     5161 gctattaccc tggcggtagc gattttgatc tcagcggtgg tgtcgctgac cctgacaccg
     5221 atgatgtgcg cgcggatgct cagccaggag tcgttgcgta aacagaaccg cttctcccgt
     5281 gcctcggaga aaatgttcga caggataatc gccgcctatg gtcgtggact ggcgaaggtg
     5341 ctgaatcatc cgtggctgac cttaagcgtg gcgctcagca cgctgctgct tagcgttctc
     5401 ttatgggtgt tcattccgaa aggtttcttc ccggtacagg acaacggcat tattcagggc
     5461 actttgcagg caccgcaatc cagctccttt gccaatatgg cccagcgaca acgccaggtc
     5521 gcggacgtga ttttgcagga tccggcagtg caaagcctga cctcatttgt tggcgttgat
     5581 ggcactaacc cgtcgctgaa tagtgcacgt ttacaaatca acctcaaacc gttggatgaa
     5641 cgtgatgatc gggtgcaaaa agtcatcgcc cgtctgcaaa cggcggtaga taaagtgccg
     5701 ggcgtcgatc tcttcctgca accaacgcag gatctgacta ttgatactca ggtcagccgc
     5761 acccagtacc agtttacctt gcaggccacc tcactggatg cgctcagtac ctgggtgcca
     5821 cagttgatgg aaaaactcca gcaactgcca cagctttctg atgtctccag cgactggcag
     5881 gacaaagggc tggtggcgta tgtcaatgtt gatcgcgaca gcgccagccg tctggggatc
     5941 agcatggcgg atgtcgataa cgccctgtac aacgcgtttg gtcagcgact gatttccact
     6001 atttatactc aggctaacca gtatcgcgta gtgctggagc acaacaccga aattaccccg
     6061 ggcctcgcgg cgctggatac cattcgcctg accagtagcg acggcggcgt ggtgccatta
     6121 agctcaattg ccaaaattga gcagcgtttt gcgccgcttt ccatcaacca tctggatcag
     6181 ttcccggtaa cgaccatctc ctttaacgtg ccggataact attcgctggg cgatgcggtg
     6241 caggcgatta tggacaccga aaagacgctg aatctgccgg tggatatcac cacgcaattc
     6301 cagggcagca ccctcgcctt ccagtcggcg ctgggcagca ctgtctggct gattgtcgcg
     6361 gcggtggtgg cgatgtatat cgtgctcggc attctgtacg agagctttat tcacccgatc
     6421 accattctct cgacgctacc caccgcaggg gtcggcgcgc tgctggcatt aatgattgcc
     6481 ggtagcgaac tggatgtgat tgcgattatc ggcattattt tgctgatcgg tattgtgaag
     6541 aagaacgcca tcatgatgat cgacttcgcg ctggctgctg agcgcgagca aggcatgtcg
     6601 ccgcgcgatg caatctacca ggcttgtctg ttgcgttttc gtccgatcct gatgaccacc
     6661 ctggcggctc tgcttggcgc gctgccgctg atgttgagta ccggggtcgg cgcggaactg
     6721 cgtcgtccgt taggtatcgg catggtcggc ggtctgattg tcagccaggt gctgacgctg
     6781 ttcaccacgc cggtgattta tctgctgttc gaccgcctgg cattgtggac caaaagtcgc
     6841 tttgctcgcc atgaagagga ggcgtaagtg aagttttttg ccctcttcat ttaccgtccg
     6901 gtggcgacga ttttactgtc ggttgccatt accctgtgcg gcatactggg cttccgtatg
     6961 ctgccggtcg ccccgctgcc gcaggtcgat tttccggtga ttatggtcag cgcctcgctg
     7021 cccggtgcgt caccagaaac aatggcgtct tccgttgcca cgccgctgga gcgctcactt
     7081 gggcgcattg ccggagtcag tgaaatgacc tccagcagtt cgctcggcag cacgcgtatt
     7141 attttgcagt ttgattttga ccgggatatc aacggcgcgg cgcgcgatgt gcaggcggcg
     7201 atcaacgctg cacaaagttt gctgcccagc gggatgccca gccgtccgac ctatcgcaaa
     7261 gctaacccgt cggatgcgcc aattatgatc ctcacgctga catccgatac ttattcgcag
     7321 ggtgaactgt acgatttcgc ctcgacgcag ctggctccga cgatttcgca aatcgacggt
     7381 gttggtgatg tcgatgtcgg cggcagctca ctgcccgccg tacgcatcgg gctgaatccg
     7441 caggcgctgt ttaatcaggg cgtctcgctg gacgacgtac gcactgccat cagcaatgcc
     7501 aacgtgcgta aaccgcaggg ggcgctggaa gatggcactc accgctggca gatccagacc
     7561 aatgatgagc taaaaaccgc cgccgaatat cagccgctga ttattcatta caacaacggc
     7621 ggcgcggttc gtctgggcga tgtggcgaca gtgaccgact ctgtacagga tgtacgtaac
     7681 gccgggatga ccaatgccaa accggctatt ttgctgatga tccgcaagct gccggaagcc
     7741 aatattatcc agacggttga cagcatccgg gcaaaattac cggagttgca ggaaaccatt
     7801 ccggcggcga ttgatctgca aattgctcag gatcgctccc ccaccattcg cgcctcgctg
     7861 gaagaagtcg agcaaacgct gattatctcg gtggcgctgg tgattctggt ggtgttttta
     7921 ttcctgcgct cgggtcgcgc aactattatt cccgccgttg cggtgccggt ttcgctgatt
     7981 ggtacgtttg cggcgatgta cctgtgcggt ttcagtctca ataatctttc gttaatggcg
     8041 ctcaccatcg ctactggttt cgtggtggat gacgccatcg tggtgctgga aaacattgcc
     8101 cgtcatctgg aagcgggaat gaaaccgttg caagccgcac tgcaaggtac tcgcgaagtc
     8161 ggttttacgg tgctgtcgat gagtctgtca ctggtggcgg tgttcctgcc gctgctgttg
     8221 atgggcggat tgccgggccg actgttacgc gaatttgccg tgacgctttc tgtcgccatt
     8281 ggtatatcgt tgctggtttc tctgacatta acgccaatga tgtgtggctg gatgctgaaa
     8341 gccagcaagc cgcgcgagca aaagcgactg cgtggttttg gtcgcatgtt ggtagccctg
     8401 caacaaggct acggcaagtc actgaaatgg gtgctcaatc atccccgtct ggtgggcgtg
     8461 gtgctgcttg gcaccattgc gctgaatatc tggctgtata tctcgatccc gaaaaccttc
     8521 ttcccggagc aggacactgg cgtgttaatg ggcgggattc aggcggatca gagtatttcg
     8581 tttcaggcga tgcgcggtaa gttgcaggat ttcatgaaaa ttatccgtga cgatccggca
     8641 gtggataatg tcaccggctt tacaggcggt tcgcgagtga acagcgggat gatgtttatc
     8701 accctcaagc cacgcgacga acgcagcgaa acggcgcagc aaattatcga ccgtctgcgc
     8761 gtaaaactgg cgaaagaacc gggggcgaat ctgttcctga tggcggtaca ggatattcgc
     8821 gttggtgggc gtcagtcgaa cgccagctac cagtacacgt tgttatccga cgacctggcg
     8881 gcactgcgag aatgggagcc gaaaatccgc aaaaaactgg cgacgttgcc ggaactggcg
     8941 gacgtgaact ccgatcagca ggataacggc gcggagatga atctggttta cgaccgcgac
     9001 accatggcac ggctgggaat cgacgtacaa gccgccaaca gtctgttaaa taacgccttc
     9061 ggtcagcggc aaatctcgac catttaccag ccgatgaacc agtataaagt ggtgatggaa
     9121 gtggatccgc gctataccca ggacatcagt gcgctggaaa aaatgttcgt tataaataac
     9181 gaaggcaaag cgatcccgct gtcgtatttc gctaaatggc aaccggcgaa tgccccacta
     9241 tcggtgaatc atcagggatt atcggcggcc tcgaccattt cgtttaacct gccgaccgga
     9301 aaatcgctct cggacgccag tgcggcgatc gatcgcgcaa tgacccagct tggggtgcct
     9361 tcgacggtgc gcggcagttt tgccggcacg gcgcaggtgt tccaggagac gatgaactcg
     9421 caggtgatcc tgattatcgc cgccatcgcc acggtgtata tcgtgctggg tatcctttac
     9481 gagagttacg tacatccgct gacgattctc tccaccctgc cctcggcggg cgttggagcg
     9541 ctgttggcgc tggagctgtt caatgccccg ttcagcctaa tcgccctgat agggatcatg
     9601 ctattaatcg gcatcgtgaa gaaaaacgcc attatgatgg tcgattttgc gcttgaagcc
     9661 caacggcacg gtaacctgac gccgcaggaa gctattttcc aggcctgtct gctgcgtttt
     9721 cgcccgatta tgatgaccac cctggcggcg ctgtttggtg cgctgccgct ggtattgtcg
     9781 ggcggcgacg gctcggagct gcggcaaccc ctggggatca ccattgtcgg cggactggta
     9841 atgagccagc tccttacgct gtataccacg ccggtggtgt atctcttttt cgaccgtctg
     9901 tggctgcgtt tttcgcgtaa acctaaacaa acggtaaccg agtaaatgac agatcttccc
     9961 gacagcaccc gttggcaatt gtggattgtg gctttcggct tctttatgca gtcgctggac
    10021 accaccatcg taaacaccgc ccttccctca atggcgcaaa gcctcgggga aagtccgttg
    10081 catatgcaca tggtcattgt ctcttatgtg ctgaccgtgg cggtgatgct gcccgccagc
    10141 ggctggctgg cggacaaagt cggcgtgcgc aatattttct ttaccgccat cgtgctgttt
    10201 actctcggtt cactgttttg cgcgctttcc ggcacgctga acgaactgtt gctggcacgt
    10261 gcgttacagg gcgttggcgg cgcgatgatg gtgccggtcg gcagattgac ggtgatgaaa
    10321 atcgtaccgc gcgagcaata tatggcggcg atgacctttg tcacgttacc cggtcaggtc
    10381 ggtccgctgc tcggtccggc gctcggcggt ctgctggtgg agtacgcgtc gtggcactgg
    10441 atctttttga tcaacattcc ggtggggatt atcggtgcga tcgccacatt gctgttaatg
    10501 ccgaactaca ccatgcagac gcggcgcttt gatctctccg gatttttatt gctggcggtt
    10561 ggcatggcgg tattaaccct ggcgctggac ggcagtaaag gtacaggttt atcgccgctg
    10621 gcgattgctg gcctggtcgc agttggcgtg gtggcactgg tgctttatct gctgcacgcc
    10681 agaaataaca accgtgccct gttcagtctg aaactgttcc gtactcgtac cttttcgctg
    10741 ggcctggcgg ggagctttgc cggacgtatt ggcagtggca tgttgccctt tatgacaccg
    10801 gttttcctgc aaattggcct cggtttctcg ccgtttcatg ctggactgat gatgatcccg
    10861 atggtgcttg gcagcatggg aatgaagcga attgtggtac aggtggtgaa tcgctttggt
    10921 tatcgtcggg tactggtagc gaccacgctg ggtctgtcgc tggtcaccct gttgtttatg
    10981 actaccgccc tgctgggctg gtactacgtt ttgccgttcg tcctgttttt acaagggatg
    11041 gtcaactcga cgcgtttctc ctccatgaac accctgacgc tgaaagatct cccggacaat
    11101 ctggcgagca gcggcaacag cctgctgtcg atgattatgc aattgtcgat gagtatcggc
    11161 gtcactatcg ccgggctgtt gctgggactt tttggttcac agcatgtcag cgtcgacagc
    11221 ggcaccacac aaaccgtctt tatgtacacc tggcttagca tggcgttgat catcgccctt
    11281 ccggcgttca tctttgccag agtgccgaac gatacgcatc aaaatgtagc tatttcgcgg
    11341 cgaaaaagga gcgcgcaatg aagttctggc gacccggtat taccggcaaa ctgtttctgg
    11401 cgattttcgc cacctgcatt gtcttgctga tcagtatgca ctgggcggtg cgtatcagtt
    11461 ttgagcgtgg ctttattgat tacatcaagc atggtaatga acagcgatta caactgttaa
    11521 gtgatgcgct tggcgagcag tatgcgcagc atggcaactg gcgcttcctg cgcaacaatg
    11581 atcgctttgt ctttcagatc ctgcgttcat ttgaacacga taattcggaa gataaacccg
    11641 gcccgggtat gccaccgcac ggctggcgta cccagttctg ggtggttgat caaaacaaca
    11701 aagtgctggt tggtccgcga gcgccgattc cacctgacgg tacacggcga cccattctgg
    11761 tcaacggtgc ggaagttggc gcggtgatcg cctcccccgt tgagcggtta acgcgcaata
    11821 ctgatatcaa tttcgataaa caacagcggc aaaccagctg gttgattgtc gccctggcaa
    11881 cgttactcgc ggcacttgcc acttttctgc tggcgcgcgg tttactggca ccggtaaaac
    11941 gacttgtcga tggcacgcac acactggcgg cgggcgattt cactacccgc gtaacgccca
    12001 ccagtgaaga tgaactgggc aaactggcgc aagacttcaa ccagcttgcc agcacactgg
    12061 agaaaaacca gcaaatgcgg cgcgatttta tggccgatat ttctcacgaa ctgcgtacgc
    12121 cattagcggt gctgcgcggt gaactggaag ccattcagga tggcgtgcgt aaattcacgc
    12181 cggagacggt ggcgtcttta caggcggagg tcggtacact gaccaaactg gttgacgatc
    12241 tccatcagtt gtcgatgtct gatgaaggcg ctctcgccta tcaaaaagca ccggtagatt
    12301 tgatcccact gctggaagtg gcgggcggcg catttcgcga acgattcgcc agtcgtggcc
    12361 tgaaactgca attttccctg ccagacagta ttaccgtatt tggcgatcgc gaccgtttaa
    12421 tgcagttatt caataactta ctggaaaaca gcctgcgcta cactgacagc ggcggcagcc
    12481 tgcaaatctc tgccgggcag cgcgacaaaa cggtgcgcct gacctttgcc gacagtgcgc
    12541 caggtgtcag tgacgatcag ctacaaaaat tgtttgaacg tttttatcgc accgaaggtt
    12601 cccgcaaccg tgccagcggc ggttccgggc tggggctggc gatttgcctg aacattgttg
    12661 aagcacataa tggtcgcatt attgctgccc attcgccttt tggcggggta agcattacag
    12721 tagagttacc gctggaacgg gatttacaga gagaagtatg accgagttac caatcgacga
    12781 aaacacaccg cgtattttga tcgtggaaga tgaaccgaag ctggggcagt tgctcattga
    12841 ttatctgcgt gctgcgagct atgcgccgac gcttatcagc cacggcgatc aggtactgcc
    12901 gtatgtgcgc cagacaccac cggatctgat cctgttagat ctgatgctcc ctggcaccga
    12961 tggcctgacg ctgtgccggg aaattcgtcg tttttctgac attccgatcg tgatggtgac
    13021 ggcaaaaatc gaagagatcg atcgcctgct ggggctggag attggcgcag atgattatat
    13081 ctgtaagccg tacagcccac gggaagtggt agcgcgcgtc aaaaccattt tgcgccgttg
    13141 caaaccgcag cgcgagttgc agcaacagga tgctgaaagc ccgttgatta tcgacgaagg
    13201 tcgttttcag gcttcatggc gcggtaaaat gcttgacctg acgcctgcgg aatttcgtct
    13261 gctgaaaacg ctctctcatg aaccaggaaa agtgttctcc cgcgagcatt tgctcaatca
    13321 tctttatgac gactaccgcg tagtaaccga ccgcaccatc gacagccaca ttaaaaacct
    13381 gcgccgcaag ctggaatctc tcgacgccga acagtcattt atccgcgccg tttacggcgt
    13441 cggttaccgc tgggaagccg acgcctgccg catcgtttag ttttagcgac attattttgt
    13501 tagccggaga tggcgttcag acccgatccg gcaacattat cccacgcatg gtcagcaaac
    13561 tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac
    13621 agcaccttta gatgctgttt tcgatacaca atttcaatca aggagtcatt atggctggtt
    13681 ggtttgaact cagtaagagc agtgataatc agttccggtt tgtgctaaaa gcgggcaatg
    13741 gtgagactat cctcaccagc gagctgtata cctcaaaagc ctctgcggaa aagggcatcg
    13801 cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagtaacg
    13861 gcaaattcta tttcaatctg aaagccgcta atcatcaaat tatcggctcc agccagatgt
    13921 acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc
    13981 agacggtgaa agacaatacg taattacgat gccgggcgtg ggttgttggc gtccggcttt
    14041 atatccaaat aacgcttagt tattaataat taagagtaac cgccgccatt aaaatagtgg
    14101 gcactgacga gcactcgttg tgctttcttt gtttaccatc ccatacttat cctcctgaac
    14161 atgttcatgt gccaaaatat cttctaatac tttcgtgacg ctatccggtt tatgacatac
    14221 actgatgtaa gatttttact tacaggcggg cgtaaaatgg ccagaacgat gacggttgat
    14281 ctcggcgatg agttgcgtga atttatcgag tccttaattg aatccggcga ctatcgcacc
    14341 caaagcgaag tgatccgtga gtcactgcga ttacttcgag aaaagcaagc agagtctcgc
    14401 ctgcaagcgt tacgtgacat gctcgcagaa gggttgagta gtggtgaagc tcagccgtgg
    14461 gaaaaggacg cgttcttacg gaaggtgaaa gcagggataa ggaaataatg tgcgtattat
    14521 taaactcatg ccaaaagcca atgaggattt agaaggcatc tggtattaca gctatcacca
    14581 tttcggcgaa ccacaagccg acagatatgt ggaacatctt tcagacgttc tccagattct
    14641 gagtaacaat aatataggta cgcccagacc tgaattaggc gaaggtattt ttgttcttcc
    14701 ttttgaacgc cacgtcattt atttcctgca atcaccaggg gaaattatcg tcattcgtat
    14761 ccttaaccaa aaccaggacg ccaccagaca tttacactgg agctaaaccc gccgattgcg
    14821 ctacaatgcc cgcccttaaa gtgggggcac tcccctaacc gcttcatcag gtgaagcgga
    14881 tctgacctgt catcagaacg agagaattat gtttaaaccg gaactccttt ccccggcggg
    14941 aacgctgaaa aatatgcgtt acgctttcgc ttatggcgca gatgctgttt atgcgggcca
    15001 gccgcgttat tccctgcgtg tgcgcaacaa cgaattcaac cacgaaaatc ttcagctcgg
    15061 catcaatgaa gcccacgcgc tggggaaaaa gttttatgtc gtggtcaaca ttgcaccgca
    15121 caacgccaag ctgaaaacct ttatccgtga cctgaaaccg gtggtggaaa tggggccgga
    15181 tgcgctgatt atgtccgatc cagggctgat tatgctggtg cgtgagcact tccctgaaat
    15241 gccgatccac ctttcggtgc aggctaacgc cgtgaactgg gcgacggtga aattctggca
    15301 gcaaatgggc ctgacccgcg tgatcctctc tcgcgagctg tcgctggaag agattgaaga
    15361 gatccgcaat caggtgccgg atatggagat cgagatcttc gttcacggcg cgctgtgcat
    15421 ggcctactcc ggtcgctgcc tgctctctgg ctatatcaac aagcgcgacc cgaaccaggg
    15481 cacctgcacc aacgcctgcc gctgggagta caacgtccag gaagggaaag aagatgatgt
    15541 tggcaacatc gtacacaagt acgagccgat tccggtgcaa aatgttgagc cgacgctggg
    15601 tatcggcgca ccaaccgaca aagtgtttat gatcgaagag gcccagcgtc cgggcgagta
    15661 tatgaccgcg tttgaagatg agcacggcac ttacatcatg aactcgaaag atctgcgcgc
    15721 catcgcccat gtagaacgcc tgaccaaaat gggcgtgcat tcgctgaaaa tcgaaggtcg
    15781 taccaaatct ttctactatt gtgcacgcac cgcacaggtt taccgcaaag ctatcgatga
    15841 cgccgctgcg ggaaaaccgt tcgataccag cctgctggaa actctggaag gtctggcgca
    15901 tcgtggctat accgaaggtt tcctgcgtcg tcatactcac gacgattatc agaactacga
    15961 atacggttat tcagtttctg accgccagca gtttgttggt gagtttaccg gtgagcgcaa
    16021 gggggacctc gcggcggtag cggtgaaaaa taaattctcc gttggcgaca gccttgagct
    16081 gatgacgccg caaggcaaca ttaattttac ccttgagcac atggaaaacg ccaaaggcga
    16141 agctatgccg atagcaccag gcgatggtta tactgtgtgg ctcccggtcc cgcaggatct
    16201 tgagctcaat tacgcgctgc tgatgcgtaa tttctccggg gaaaccacgc gtaatcccca
    16261 cggtaagtga ttaatttcga ttatttttcc cggatggaaa attcttagaa accgatcaca
    16321 tacagctgca tttattaagg ttatcatccg tttcgctgaa aaacataacc cataaaatgc
    16381 tagctgtacc aggaaccacc tccttagcct gtgtaatctc ccttacacgg gcttattttt
    16441 ttatgcataa gccctatctc tggtaaccgt cttccattga ccacatcgat agaatcctcc
    16501 ttcatagcac gatgcctttc acttatcggc atcgtgctcc cacaggttcc ggctacgcac
    16561 agccagaacg cgcatatttg acgcttacca aaaaatattc tcactctcca catttgaatg
    16621 tcagacgagc gacaccatgt aatcctacac cttctgtctt cagctcaact atttgcattt
    16681 ttttgccctg agtaacacag aaatgagttg catcattttt tactatattt tctgcaccag
    16741 atattctacc cctggctaaa gaagcttcgg cttcggtgta gtattggtta tcgagtttac
    16801 gctgaatatt acttttatat gcaagaccaa atttaccgat acttgtctca tcattatgca
    16861 cagcacaacc agacataata aaaatactaa ttaatgatat agcagctatc tttttcatct
    16921 cacctttccc cattaaatac caacgacact ctctagtgtt taaatataat aatg
//
LOCUS       DAZKHC020000088        16807 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZKHC020000088 DAZKHC020000000
VERSION     DAZKHC020000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.088"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..764
                     /locus_tag="ACHOSM_RS17760"
                     /old_locus_tag="pgaptmp_003552"
     CDS             <1..764
                     /locus_tag="ACHOSM_RS17760"
                     /old_locus_tag="pgaptmp_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HGU4559988.1"
                     /translation="RYKLLKKDARIVAAQQVARQESAMCLRRRWSPENFKLFLVEHPL
                     VRHLTRRLIWGVYSAENQLLACFRVAEDNSYSTADDDLFTLPEGDISIGTPHVLEISP
                     TDAAAFGQLFADYELLPPFRQLDRNSYALTEAERNASELTRWAGRKCPSGRVMGLANK
                     GWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAVGMSPAELSAEQLLSKLWLWEGKA
                     ESYGWGSNSTQEAQFSVLDAITASELINDIEALFE"
     gene            774..4406
                     /gene="yehI"
                     /locus_tag="ACHOSM_RS17765"
                     /old_locus_tag="pgaptmp_003553"
     CDS             774..4406
                     /gene="yehI"
                     /locus_tag="ACHOSM_RS17765"
                     /old_locus_tag="pgaptmp_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HGU4559989.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRKWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSVPARTMAALACIAQ
                     NDNQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVATDPKAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQAQSVVKRYLSASPKPVIDSTDNSGLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPSLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRKKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEADLRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            4468..4785
                     /gene="yehK"
                     /locus_tag="ACHOSM_RS17770"
                     /old_locus_tag="pgaptmp_003554"
     CDS             4468..4785
                     /gene="yehK"
                     /locus_tag="ACHOSM_RS17770"
                     /old_locus_tag="pgaptmp_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HGU4559990.1"
                     /translation="MIVQKELVAIYDYEVPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELLNIFNQ
                     EIE"
     gene            5092..6181
                     /gene="yehL"
                     /locus_tag="ACHOSM_RS17775"
                     /old_locus_tag="pgaptmp_003555"
                     /pseudo
     CDS             5092..6181
                     /gene="yehL"
                     /locus_tag="ACHOSM_RS17775"
                     /old_locus_tag="pgaptmp_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            6192..8471
                     /gene="yehM"
                     /locus_tag="ACHOSM_RS17780"
                     /old_locus_tag="pgaptmp_003556"
     CDS             6192..8471
                     /gene="yehM"
                     /locus_tag="ACHOSM_RS17780"
                     /old_locus_tag="pgaptmp_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HGU4559991.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQIALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQRNNGDVLVVCGGWHAPALAKMWRECPQEIN
                     KPELPSLANAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWLWGLQQAGEQLLKT
                     VLTRLRQHNLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVFLSAAVALLERRSQAIHVPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            8464..9521
                     /gene="yehP"
                     /locus_tag="ACHOSM_RS17785"
                     /old_locus_tag="pgaptmp_003557"
                     /pseudo
     CDS             8464..9521
                     /gene="yehP"
                     /locus_tag="ACHOSM_RS17785"
                     /old_locus_tag="pgaptmp_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            9518..11521
                     /gene="yehQ"
                     /locus_tag="ACHOSM_RS17790"
                     /old_locus_tag="pgaptmp_003558"
     CDS             9518..11521
                     /gene="yehQ"
                     /locus_tag="ACHOSM_RS17790"
                     /old_locus_tag="pgaptmp_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HGU4559992.1"
                     /translation="MNPLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSNDPFANDEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            11646..12107
                     /gene="yehR"
                     /locus_tag="ACHOSM_RS17795"
                     /old_locus_tag="pgaptmp_003559"
     CDS             11646..12107
                     /gene="yehR"
                     /locus_tag="ACHOSM_RS17795"
                     /old_locus_tag="pgaptmp_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HGU4559993.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSTNLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(12147..12617)
                     /gene="yehS"
                     /locus_tag="ACHOSM_RS17800"
                     /old_locus_tag="pgaptmp_003560"
     CDS             complement(12147..12617)
                     /gene="yehS"
                     /locus_tag="ACHOSM_RS17800"
                     /old_locus_tag="pgaptmp_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HGU4559994.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(12664..13383)
                     /gene="btsR"
                     /locus_tag="ACHOSM_RS17805"
                     /old_locus_tag="pgaptmp_003561"
                     /gene_synonym="yehT"
     CDS             complement(12664..13383)
                     /gene="btsR"
                     /locus_tag="ACHOSM_RS17805"
                     /old_locus_tag="pgaptmp_003561"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HGU4559995.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(13380..15065)
                     /gene="btsS"
                     /locus_tag="ACHOSM_RS17810"
                     /old_locus_tag="pgaptmp_003562"
     CDS             complement(13380..15065)
                     /gene="btsS"
                     /locus_tag="ACHOSM_RS17810"
                     /old_locus_tag="pgaptmp_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HGU4559996.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            15287..15352
                     /locus_tag="ACHOSM_RS17815"
                     /old_locus_tag="pgaptmp_003563"
                     /pseudo
     CDS             15287..15352
                     /locus_tag="ACHOSM_RS17815"
                     /old_locus_tag="pgaptmp_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15576..16225)
                     /locus_tag="ACHOSM_RS17820"
                     /old_locus_tag="pgaptmp_003564"
                     /pseudo
     CDS             complement(15576..16225)
                     /locus_tag="ACHOSM_RS17820"
                     /old_locus_tag="pgaptmp_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4228 a         4333 c         4194 g         4052 t
ORIGIN      
        1 accgctacaa attgctgaaa aaagatgcgc gtatcgtcgc cgcccagcag gtagcaaggc
       61 aggaatccgc catgtgcctg cgtcgccgct ggtcgccaga aaactttaag ctcttcctgg
      121 ttgagcatcc gttggttcgc cacttaaccc gccgtctgat ttggggcgtt tatagcgccg
      181 aaaaccagct actggcttgc tttcgcgtag cagaagataa cagctacagc accgctgacg
      241 atgatctttt caccctgccg gaaggcgata tctctatcgg cactcctcac gttctggaaa
      301 tatcaccaac ggatgctgcc gcctttggtc agctttttgc cgactacgaa ctgctaccac
      361 cgttccgcca gctcgaccgt aacagctacg ccctgacaga ggccgagcgc aatgccagtg
      421 aactgacccg ctgggcaggc agaaaatgcc cgagtggtcg ggtcatgggg ctggcgaata
      481 aaggctggat aaagggcgaa ccacaggatg gaggctggat cggatggatg atcaaacctt
      541 tgggtcgctg gtcgttaatc atggaaatcg acgaaggttt tgcggtgggt atgtcaccag
      601 ccgaacttag cgctgagcag ctcttaagca agctgtggtt atgggaaggc aaagcggaaa
      661 gctatggctg ggggagtaat tcaacacagg aagcgcagtt ctccgtactc gatgccatca
      721 ccgccagcga gctaattaac gatattgaag ctctgtttga ataaggaaag agcatggaca
      781 aggaattacc gtggctggcg gataacgccc aactggaact gaaatataaa aaaggcaaaa
      841 caccactcag tcatcgcaaa tggccgggcg aaccggtgcc tgttatcact gaaagcatca
      901 ttcagacact gggtgatgaa ttgctacaaa aagctgagaa gaaaaaaaac attgtctggc
      961 gttatgaaaa tttttcactg gagtggcagt ccgccattac gcaggccatc aacttgatcg
     1021 gcgaacacaa accctcagtc ccggcccgca caatggcggc actagcctgt atcgcgcaaa
     1081 atgacaacca acagttgctc gacgaaatcg tccaacaaga ggggctggaa tatgcgactg
     1141 aggtggtgat tgcacgccag tttattgcac ggtgttatga gagtgatcct ctggtagtca
     1201 cattgcagta tcagaacgag gactatggct atggttatcg ctcagaaacc tataacgaat
     1261 tcgatctccg actgcgtaaa cacctctctc tggcagagga aagctgctgg cagcgttgcg
     1321 ccgacaaact cattgccgca ctgccaggaa taaccaaagt tcgccgccct tttattgcgc
     1381 tgatcctccc cgaaaaacca gaaattgcca atgagttggt aagccttgaa tgcccgcgga
     1441 ctcattttca ttctaaggag tggttaaaag ttgttgctac tgaccccaaa gcggtgagaa
     1501 aactcgaacg ctactggagc caggatatat ttagcgatcg agaagccagc tacatgtcgc
     1561 atgaaaacca cttcggctac gcggcctgcg ccgccctttt gcgcgaacaa ggactggcag
     1621 ccgttccgcg cctcgcgata tatgcccata aagaagattg cggcagtctg ctggtacaaa
     1681 ttaaccatcc gcaagtcatc cgcaccttgc tgttagttgc tgataaaaac aaacccagcc
     1741 tgcaacgtgt ggctaaatac agtaaaaact tcccccatgc gacactcgcc gcactggcag
     1801 aactgctggc gttaaaagaa ccaccagccc gcccaggtta tccaatcatc gaagacaaaa
     1861 agctgcctgc acagcaaaaa gctcgagatg aatactggcg tacgctgtta caaacgctga
     1921 tagcatcgca gccacaacta gcagaaaggg tgatgccgtg gttaagtact caagcccagt
     1981 cagtggtgaa gcgttattta tcggcatcgc caaaaccggt tattgatagc accgataaca
     2041 gcggtctgcc tgaaatgctc gtttcaccac cgtggcgtag taagaaaaaa atgacagctc
     2101 cacgtcttga tttggcaccg ctcgaattaa ctccgcaagt ttactggcaa ccaggcgaac
     2161 aagagaggct tgccgccact gagtctgccc gttatttcag cacggaatct cttgcggaac
     2221 gcatggaaca aaaaagtgga cgagttgtat tacaggaact gggttttggg gatgatgtat
     2281 ggctgtttct gaattatata ctccccggaa aactggatgc tgcacgcaat tcactcattg
     2341 ttcagtggca ttactaccag gggcgggttg aagagatcct gaatggctgg aactccccgg
     2401 aagcacaatt agcagaacag gcgctccgca gcggtcacat agaagcgtta attaacatat
     2461 gggaaaatga caactactca cgttatcgtc cggaaaagag tgtctggaac ctgtatttat
     2521 tggcacagtt gccgcgtgag atggctttga ccttctggct gcgtatcaat gagaaaaagc
     2581 atctgttcgc gggtgaggac tattttctca gtatcctcgg attggatgcg ctaccaagtc
     2641 tgctgttggc tttttcacat cgtccaaaag aaacatttcc gttaatttta aattttggcg
     2701 caacagaact ggcgctgcct gttgcccgcg tctggcgacg tttcgcggcg cagcgtgatc
     2761 tggctcgcca gtggatttta caatggccgg aacatacggc tagtgcactt atccctcttg
     2821 tctttaccaa acccagcgat aatagcgaag ccgcattact tgccctgcgt ttactgtacg
     2881 aacagggaca tggcgaattg ctacaaaccg tggcaaaccg ctggcagcgt acagatgtat
     2941 ggtctgccct ggagcagttg cttaaacagg gtccaatgga catttacccg gcacgcattc
     3001 caaaagcccc tgatttctgg catccggcaa tgtggtccag gcctcgactt atcactaata
     3061 atcagcctgt taccggtgac gctctggaaa ttatcggcga aatgctgcgc tttacccagg
     3121 ggggacgttt ttatagcggg ctggaacaac tgaaaacgtt ctgccagcca caaacgctgg
     3181 cagcttttgc ctgggatctc ttcactgcgt ggcaacaagc tggtgccccc gcaaaagaca
     3241 actgggcatt tctggcgtta agtctctttg gtgacgaaag cacggcacgg gatctgacga
     3301 cacagatcct cgcctggcca caagaaggca aatctgcccg tgctgtcagc ggcctgaaca
     3361 tccttaccct gatgaataat gatatggcgc tgatacagct gcatcatata tcgcaacggg
     3421 ctaaatcccg ccctttacgt gataacgcgg cggaatttct tcaggtggtc gcagaaaatc
     3481 gcgggctaag ccaggaagag ctagcggaca gattagtccc aaccctgggc cttgatgatc
     3541 cgcaggcgtt gagttttgat tttggtcccc ggcagtttac cgttcgcttc gatgaaaacc
     3601 tcaacccggt tatctttgat cagcaaaacg ttcgcaagaa aagcgttccc cggttgcgcg
     3661 ccgatgacga tcaactgaaa gcgcccgagg cactggcccg actaaaaggg ctaaaaaaag
     3721 atgctactca ggtgagcaaa aacctgctcc cgcgtcttga agctgaccta cgtaccaccc
     3781 gacgctggtc gctggcagat tttcattctc tgtttgttaa tcatcccttt acccgtctgg
     3841 ttacccagcg attaatatgg ggggtttatc cggcaaatga accgcgttgt ttactcaaag
     3901 cctttcgtgt ggccgcagag ggggagttct gcaatgcgca agatgagcca attgacctgc
     3961 ctgcggacgc tctgattggc attgcccacc cgttagaaat gacagtagaa atgcgcagtg
     4021 aatttgcaca gctttttgcc gattacgaaa ttatgccgcc ttttcgccag ttgtcgcgcc
     4081 gcacggtgct gctcacacct gacgagtcaa ccagtaacag cctgactcgc tgggaaggta
     4141 aatccgctac cgttgggcag cttatgggaa tgcgatacaa aggctgggag tcaggttatg
     4201 aggacgcatt tgtctatgac ctgggtgagt accggctggt ccttaagttt tcacccggtt
     4261 ttaaccacta caatgttgat agcaaagcgc taatgagctt ccgttctctt cgagtgtacc
     4321 gtgacaataa atccgtcact tttgccgaac ttgatgtgtt tgatttgagt gaggcgttaa
     4381 gcgcacctga cgtcattttc cattaaaaac acagcgggca gtgatgcaac tgcccgttat
     4441 caacacgact taccagcggg gaaagcgatg atcgtgcaaa aagagctggt tgctatttac
     4501 gattatgagg tccctgtacc tgaaaatccg ttttccttca gacttgagat ccataaatgc
     4561 tctgaattat ttacaggttc cgtctatcga ctggagcgat tccggctacg tccaacattt
     4621 catcaacgtg atcgagaaga tgctgacccg ctaataaatg atgcgttgat ttatataaga
     4681 gatgagtgta ttgatgagcg gaaattacga ggtgaatcac ctgaaactgt aatagcaatt
     4741 tttaatcgtg aactactgaa tatattcaac caagaaatag aataatatac tctaaataat
     4801 tcaaattggt ccgatccggc gcaacgtccc aatggcctgg attataaatc tcattatctt
     4861 aattgcaacg gggtccagcc gtggttatag ttcgttcgtt ggaacacgat gttgcacagg
     4921 ctgcggtgtt gggcctgaaa atagtaaata aaagaacctg cctcaccagc aggttttttt
     4981 atttactgtg atctgctttc cagatatttt ccgctcaaac aactaatgcg ccaaacattt
     5041 attgcgcgta aaatatcgtt tatttcatta atacatttca gggatgaata tatgtcaccg
     5101 cagaataatc atctgcagcg tccgcctgct gctgtgttat acgccgatga actggcaaaa
     5161 ttaaaacaaa atgataacgc accttgcccg cccggttggc agttaagttt gcctgcggca
     5221 cgtgctttta tccttggcga tagcgcgcaa aatattagcc gtaaagtggt gattagtccc
     5281 tctgctgtcg aacgcatgtt agtcactctc gctaccgggc gcggtttgat gttggtgggg
     5341 gaaccgggta ccgcaaaatc tctcctgtct gaattactgg caaccgctat tagcggcgat
     5401 gccgggttaa ccatccaggg cggcgcatct accactgaag atcaaatcaa gtacggctgg
     5461 aactacgctc tgcttatcaa tcatggccca tcaacggaag cgctggtccc tgccccgctt
     5521 taccagggaa tgcgcgacgg caaaatcgtc cgtttcgaag agataacgcg tacaccgctg
     5581 gaagtgcaag attgtctgtt aggtatgctt tctgacagag ttatgacggt gccggaactc
     5641 actggtgaag ccagtcaact ctatgcgcgg gaaggcttta atattattgc cactgccaat
     5701 acccgcgatc gcggcgtcaa tgagatgagt gccgcgctaa agcgtcgctt cgattttgaa
     5761 actgtctttc cgattatgga tttcgctcag gagctggagc ttgtcgccag tgcttcggcg
     5821 cgtttgctgg cccatagcgg tattccgcat aaagtgcctg acgcggtact ggagttactg
     5881 gtccgcacct tccgcgatct gcgtgccaac ggcgaaaaga aaacgtcaat ggataccctg
     5941 atggcaatta tgtccaccgc tgaagccgtg aacgtggccc acgccgtagg cgtccgcgcc
     6001 tgggttttta gcgaatcgcg caggtgagcc agccgacctg gtggactgca ttgccggaac
     6061 catcgtcaaa gataacgaag aagatcgcgc tcgtctgcgc cgttacttcg aacaacgagt
     6121 cgccacacat aaagaagctc actggcaggc ttattatcaa acccgccacc gcctgccgtg
     6181 aggaaagatg catgagcgag ccgttaattg tcggcatccg gcatcatagt ccggcctgcg
     6241 cccggctggt gaaatcgtta atcgaaagcc agcggccacg atacgtattg attgaaggcc
     6301 cggctgattt taatgaccgg gtagacgaac tgtttttagc ccaccagctt ccggtagcta
     6361 tttacagtta ttgccagtat caggacggtg cagcccccgg gcgtggtgcc tggacgccat
     6421 ttgctgaatt ttcgccggag tggcaggcgc tacaagccgc acgtcgtatt caggcacaaa
     6481 cttacttcat cgatttgcct tgctgggcgc agagtgaaga agtggacgat tctcctgata
     6541 cgcaagagga aagccaggcc ttgctgctgc gtgccacccg catggataac agcgacaccc
     6601 tgtgggatca cttgttcgaa gatgaaagcc agcaaattgc attaccctct gcgctggcgc
     6661 actattttgc ccaactgcgg ggcgatttcc ccggcgatgc actcaatcgt cagcgcgaag
     6721 cctttatggc tcgctggatt gcatgggcgg tgcagcgaaa taatggcgac gtgttagtcg
     6781 tctgcggtgg ctggcacgct cctgcactgg caaaaatgtg gcgcgaatgt ccgcaggaaa
     6841 ttaacaagcc agaattgccc tcgctggcaa atgccgttac aggttgttat ctcaccccct
     6901 acagtgaaaa gcgccttgat gtgctggcag gatacctttc cggtatgcct gccccggtct
     6961 ggcaaaactg gtgctggctg tggggcttac agcaggccgg tgaacaactg ctaaaaacgg
     7021 ttctcacccg tttgcgccag cacaacttgc ctgcttcgac cgcggatatg gctgccgcac
     7081 atctgcatgc aatggcactg gcacagttgc gcggtcatac actaccgtta cgcactgact
     7141 ggctagatgc catagcaggc tcgctgatta aagaagccct gaatgcaccg ttgccgtgga
     7201 gctatcgcgg cgttattcat cccgataccg atccgattct gctaacgttg atagacacat
     7261 tagcgggtga cggattcggt aaacttgccc cttctacgcc gcaaccgcct ctgccaaaag
     7321 atgtcacctg cgaactggaa cgtaccgcaa tctctcttcc ggcggagctt accttaaatc
     7381 gctttaaccc caatggacta gcgcaaagtc aggtgttaca tcggctggca atactggaga
     7441 tcccagggat tgtacgccag cagggaagca cactgacact tgcaggcaac ggtgaagaac
     7501 gctggaaatt aacccgcccg cttagccagc atgcggcatt gattgaggcc gcctgctttg
     7561 gtgccacact ccaggaagcc gcacgccata aattagaagc cgatatgctg gacgcgggtg
     7621 gaatcggcag tatcaccaca tgtcttagcc aggcggcgtt agcgggtctg gcgtccttca
     7681 gtcaacaatt actggagcaa ctcacattat taatcgccca ggaaaatcaa tttgccgaaa
     7741 tgggccaggc gctggaagtg ctatatgcct tatggcggct ggatgaaatt agcggtatgc
     7801 aaggcgcgca gatattacaa acgacgttat gcgcggctat cgatcgcacg ctgtggctgt
     7861 gtgaatctaa cggcagacca gatgaaaagg agtttcacgc tcacctgcat agctggcagg
     7921 cactttgcca tatcctgcgc gatctacata gcggcgttaa tttacccggc gtttttcttt
     7981 ctgcggcggt agccttactg gagcgccgca gtcaggcgat tcatgtcccg gcgctggatc
     8041 gcggcgcggc tcttggcgca ctgatgcgtc tggaacatcc caacgccagt gccgaagcgg
     8101 cgctgacgat gctggcgcag ttatccccgg cacaatccgg cgaggcgctg cacggtttgc
     8161 tggcattagc ccgtcatcaa ctggcctgtc agccggcatt tatcgccggt ttcagcagtc
     8221 atttaaatca actaagtgat gccgatttta ccaatgccct gcccgattta cgcgcggcga
     8281 tggcctggct accgccacga gaacgcggga cgctggcgca tcaggtgctt gagcattacc
     8341 agcttgcgca gcttcctgtt tcagcgctgc aaatgccgtt gcattgtcct ccgcaagcca
     8401 ttgcacatca tcaacaactc gaacagcagg cactggcatc gctgcaaaac tggggagttt
     8461 tccatgtctg aactgaacga tcttctgacc acccgtgagc tacaacgctg gcgattaatt
     8521 cttggcgaag cggcagaaac gacgctttgt gggctggatg acaacgcccg gcagatagac
     8581 cacgcgctgg aatggctgta tgggcgcgat cctgaacggc tccagcgtgg tgaacgctcc
     8641 ggtggattag gtggctcaaa tctcaccact cctgagtgga tcaacagtat tcacacgctg
     8701 tttccgcaac aggtgattga gcggctggaa agcgatgccg tactgcgcta cggcattgaa
     8761 gatgtggtga cgaatctcga catgctggaa cgtatgcagc cttctgaaag cctgctacgc
     8821 gccgttttgc acaccaaaca tctgatgaat cccgaagtac tggctgccgc ccgccggata
     8881 gtgcgccagg ttgttgaaga aattatggct cgactggcaa aggaagtact ctgcgcgcta
     8941 acctgcaaca ctggcacccg caacacggca agttgtatat cgaatccccc cgctttaaca
     9001 gccgcattaa acgccaaagc gaacaatggc aactggtctt actggttgat caaagcggat
     9061 cgatggtgga ctcggtgatc cactctgcgg tgatggcggc ctgtttgtgg cagttacccg
     9121 gcattcgtac ccatctggtg gcgtttgaca caagcgtggt tgatctcacg gcagacgttg
     9181 ccgatccggt agagttatta atgaaagtac agttgggcgg cgggaccaat atcgccagtg
     9241 ccgtggagta tggtcggcaa cttattgaac aaccagcaaa aagcgtcatt atcctcgtga
     9301 gcgattttta cgaagggggt tcatcatcat tgctgacgca tcaggtgaaa aagtgtgtcc
     9361 agagcggcat caaagtgctg ggactggctg cgctcgatag caccgcaaca ccttgctatg
     9421 accgcgatac ggcccaggcg ctggttaatg tcggcgcaca aatagccgcc atgacgccgg
     9481 gcgagctggc atcatggctt gcggagaatc ttcagtcatg aatccactac gtccggaatt
     9541 attagaactg acaccgcagg ccctgacggc gttaagcaat gccggttttg ttaagcgcag
     9601 tcttaaggaa ctggaaaatg gcaacgtccc ggagatcagc catgagaacg acgctttaat
     9661 cgccaccttc agtgacggtg tccgtaccca gctggcgaac ggccaggcac tgaaagaggc
     9721 tcagtgcagt tgcggggcca acggtatgtg ccgtcatcgc gtgatgctgg tgttaagtta
     9781 tcaacgactc tgtgccacca ctcagtctac ggaaaaagaa gaagagtggg atccggcaat
     9841 ctggctggaa gaactggcta cccttcccga tgctacccgc aaacgcgcac aggcgctggt
     9901 cgctaaaggc atcaccattg agttgttctg tacgccgggc gaaattccct ctgcccgctt
     9961 accgatgagc gatgtgcgtt tttattcccg tagcagcatt cgtttcgccc gttgtgattg
    10021 tattgaaggc acactttgcg aacatgtcgt actggcagta caggcctttg tccaggcaaa
    10081 agcgcagcaa gcggaattga ctcatttaat ctggcagatg cgcagcgaac acgtcacatc
    10141 atctaacgat ccgtttgcca acgacgaggg taacgcgtgt cgtcaatatg ttcagcaatt
    10201 aagccaggca ttatggctgg gcggcatcag ccagccgctt attcactacg aggccgcttt
    10261 tagtcgcgcg cagcaggcgg cggaacgctg caactggcga tgggtgagtg aatcactacg
    10321 acagctacgg gcaagcgttg atgccttcca cgcccgcgcc agccactatc atgccggaga
    10381 atgcttacgt cagcttgcgg cattaaacag tcgattaaat tgcgcacaag agatggcccg
    10441 gcgcgacagt attggtgaag ttcctcctgt gccgtggcga acggtcgttg gctctggcat
    10501 tgccggagaa gcaaagcttg atcatctgcg gctggtgtct ttaggtatgc gttgctggca
    10561 ggatattgag cattatggtt tacgcatctg gtttaccgat cccgacaccg gcagtatttt
    10621 gcacctttcg cgcagttggc cgcgaagtga acaggaaaac tcaccggcag ctacgcgtcg
    10681 gctgtttagt tttcaggctg gcgcactagc gggcgggcaa attgtttcac aagcagcaaa
    10741 acgcagtgcc gatggcgagc tgctgttagc tacccgcaac cgcttaagca gcgttgtgcc
    10801 gctgtcgcct gatgcctggc aaatgttgag cgcgccgtta cgccagccgg gcattgtggc
    10861 tttgcgggaa tatttacgcc agcgtccccc cgcctgcata cggcctctta atcaggtcga
    10921 taacttattt attctgccgg tcgctgagtg tatttcgctc ggttgggata gcagccgcca
    10981 gacgctggat gcgcaggtaa tcagcggcga aggtgaagat aatctgctga cgttatcact
    11041 gccagcctca gccagcgcgc cttatgccgt tgaacgcatg gcggcgcttt tgcaacaaac
    11101 agacgacccc gtgtgtctgg tttctggctt tgtcagtttt gttgatgggc aattgacact
    11161 ggaaccacag gtaatgatga caaagactcg tgcctgggcg ctggacgcag aaactgcgcc
    11221 tgtggtggta tcactacctt ctgccagcgt tctgcctgtg ccgtctactg ctcatcagtt
    11281 gctgatgcgc tgccaggcgt tacttattca actgctccat aacggctggc gctatcagga
    11341 acagagcgct attagtcagg cagagttgct ggcgaatgac ctcaccgcgg tgggttttta
    11401 tcggctggca catgtgttgg cacaatttcg taatacagaa agcgaggcac gggtagaagc
    11461 aatgaataac ggtgttttgc tttgcgaaca attattcccc atgcttcagc aacaaggatg
    11521 aaatagtgct ttttactaag agttctactc cagttccgga ctgctcacgc cacggtatta
    11581 ggcatatcct atatagcccc tggtgagagt caccagtttc ttgattaaat aaaatggagt
    11641 tttacatgaa ggctttcaat aagctgtttt ccctcgttgt tgcatctgtt ctggttttct
    11701 ctcttgctgg ctgcggtgac aaagaagaat cgaagaaatt cagcaccaat ctgaacggca
    11761 ctgaaattgc cattacctat gtctacaaag gtgacaaggt gcttaagcaa tcttctgaaa
    11821 ccaaaattca atttgcctcc attggtgcaa ccaccaaaga agatgctgcc aagacacttg
    11881 agccgttaag cgccaaatac aaaaacatcg cgggtgttga agaaaaatta acctataccg
    11941 atacctacgc gcaggaaaat gtgactatcg atatggaaaa agtggatttt aaagccctgc
    12001 agggtatttc aggaatcaac gtttctgctg aagatgccaa aaaaggtatc actatggcgc
    12061 aaatggaact ggtgatgaaa gccgctggtt ttaaagaagt gaaataatcg gttggcggtc
    12121 atgctctaaa catgaccgcc aattttttag ccttttttca catgctggcg cgctgccagt
    12181 ccacgcagaa aataacgtaa aaattgatcg ccgcattcgc ggaagttttt atgatccggt
    12241 gcgcgcatca tcgctgtaat ttccggcatc gaaacgcgga actgctgttc ggtgaggata
    12301 gccagaatgt catcggtttt cagcgaaaac gcgatgcgta attttttcag cacgatgttg
    12361 ttattaatgc gacgttccgg ctccagtgcc ggagcagact catccttgcc gcgtttttca
    12421 taaatcaggc cattgaggaa tgacgacaaa acaatgtccg gacaacgctg aaaaccctct
    12481 tcgtcttctt tacgtagcca gacggcgatc tgttccgcgg tggcttcgac attacccagc
    12541 gccagaatac gcaccaggtc attattattg gctttcaaaa tgtagcgcac gctgcgcaga
    12601 atatcgttac ttagcatgag gccttcaggt gttgatgagg caaaaagcca ttttagcagt
    12661 cttttacagg ccaatcgcct cttttaagct tttcagataa cggcggctga ccggcacggt
    12721 taagccatta cgcaaaatca actcggcctg gccgttatct tccagacgaa tctcctgtaa
    12781 atgcgcgagg ttaaccagat actgacgatg gcagcgcagt agtggtgtac gactttccag
    12841 ggtacgtaat gtcaattcgg taaagccctc tttcccttcg tggctggtaa cgtagacacc
    12901 gctcatccga ctgctgacaa atgccacatc tttcatttgc agcaaataaa tccgactatg
    12961 ccccgtacaa gggataaatt tcagcgcctg ttgattttcc ggtaacagcg aaacatcctg
    13021 cttgctgcgc tcctgacgca atcgcgccag cgttttctcc agtcgcgctt catcaattgg
    13081 cttcagcaga taatcaaagg catgttcttc aaaggcttta atagcgtatt cgtcaaacgc
    13141 agtgagaaaa acaatatacg ggcgatgttc cgggtcaagc atccccacca tttccagacc
    13201 actgatgcgc ggcatctgga tatcgagaaa cagcacatcc gggcgcagtt tatgcaccgc
    13261 gccaatccct tccacggcgt ttgaacactc tccaacgatt tcaatatcgc tctgctcctg
    13321 caaaaataca cgcaggttct cccgtgctaa cggttcatca tcgacaatta agactttaat
    13381 catgcctcgt ccctccatgg tagtcgtaac gttattcggg tgtaactatc aggctcacag
    13441 gcgacgctta ttccatagtc atcgccaaac cgttcacgta aacgcttatc caccagattc
    13501 atccccagcc cactggcatt ggttaccggt tgatacaaac cggcattgtc ttcgatctcc
    13561 agcatcaaat gttgcccctc acgtcgggcg ctgattgcca ctcgccctgt atccagcagt
    13621 tgtgatgtcc catgtttaat ggcgttttcc actatcggtt gcagggtaaa cgcgggcaat
    13681 tgctgctggg ataattcttg cggaatagca atgttgacct gcaaccgcga ctggaagcgc
    13741 gccttttcaa tttgcagata agcgttcaca tgttcaattt cgtcggcgag agtaacaaac
    13801 tccgaaggcc gctttaagtt tttgcggaaa aaagtggaaa gatactgcac cagctggctg
    13861 gcctgttcgc tgtcgcggcg gatcaccgct ttaatggtgt taagcgcatt aaacaaaaaa
    13921 tgaggattca cctgggcgtg aagcagtttg atttctgact gggtgagcat cgctttttgc
    13981 cgctcatatt gcccggcaag aatctgcgcc gaaagcagtt gcgcaatccc ctcgcccagc
    14041 gtgcggttta ttgaactgaa taaacggttt ttggcttcat acaatttgat ggtgcccatc
    14101 acgcgctgat tttcgccacg caacggaatt accagcgtcg accccagttt gcattgcgga
    14161 tgcaaagagc aacgataagg tacttcgttg ccatcagcgt agaccacttc accggtttca
    14221 atcgctttta aggtgtaagt cgaagaaatc ggtttgccgg gtaaatggtg gtcgtcacca
    14281 attccggtaa aggccagcaa tttctctcga tcggtaatcg cgactgcacc aatatccagc
    14341 tcctgataca gcacctgagc cactttcatg ctgttcactt cgttaaaccc ctgtcgcaaa
    14401 atgccttccg tcgaggctgc cactttcagc gcagtggcag aaaaagccga agtgtatttt
    14461 tcaaacatcg cgcgtttatc gagcaatata cgcataaaca gcgccgcgcc gacggtattg
    14521 gtgaccatca ttggcgcagc aatattactc accagacgca ccgcatcttc ataaggtcgg
    14581 gcgatcgcaa ggatgatcag catttgcacc atttcagcga cgaacgtgac ggcaccggcg
    14641 gtaatggggt taaagacttt atcagtgcgc ccgcgacgga tcaggatgct gtgtaccagg
    14701 ccgccgagca atccttcaac gatggttgag atcatgcaac ttagcgcggt catgcccccc
    14761 atcgaatatc gatgtaagcc gccggtcaga ccaaccagcc caccgacgac cggaccgccg
    14821 agtaagccgc ccattaccgc gcctatcgca cgggtattgg caatagaatc gtcaatgtgc
    14881 aacccaaacc aggtgcccat gatgcagaag atggaaaaga cgatgtagca gagaaattta
    14941 tgcggcagac gaaccgtgac ctgcattaac ggtatgaata atggcgtttt actcattaac
    15001 cacgcaatga ctaaaaaaac gcacatctgc tgaagcagca gcaacaccag attaaaatcg
    15061 tacatacccg caaaccacac ttccctttaa aacgcgtaac atacattgcc tgcgtttaac
    15121 tttctttgaa ctcttgcaga aaaatgagaa ttcgtgagta cgatcactca aaatcgcctg
    15181 gcaaaaataa aatcacccta tagatgcaca aaaaacgggc aaaactacct ggttcgcaaa
    15241 actgcgtcta aagttaaacc gggacctcgc gagcaagggt gagacgatgg cgctttacac
    15301 aattggtgaa gtggcgttgc tttgtgatat taaccctgtc acgttacgcg cgtgacgcaa
    15361 catttttaaa aatcaacgaa gatcgtccag gagcgccgat tttcaagggt tggacagata
    15421 attcgttttc gatgtcgacg aacttcgacg aatttcgccg ataaaaaatg tcccgtacat
    15481 gccccatagc ctatatcata aattgacact ggatatacac agataaaaat aacacctgta
    15541 cataaatggg tcttccccga tcatggtggg aagacttaga acgccatatt cagtttccca
    15601 tagtggaaca tcactccctg cttaaagcgc tcccagttgc ggtatcccct ggcttttatt
    15661 ctcagcagcc tgatcttgct gttcagtgcc tccgcatttc cgtttgatac accatagcgc
    15721 atggtgttca ggataccgta cagccttttc cctatcgttt tagccacgtt cttcatcatg
    15781 ggggacgtca ctgttggccg tcaacgtcag ccatctatgc cagtcgttgc gtctttcctc
    15841 gctccatggt ctgctccaga tatcctttgc cagttctttc agctcccagc actagctcgt
    15901 cagcttcatc tgcgcactct gccatatcaa cttttcctgc ctggattcgg tcatccattt
    15961 atcgctgtac tgccaccaga agcgcgttcc ctttgcctga cgacgacttt caacaggaag
    16021 atgcggatgt tcattctggc gggttttatc catcacctcc accagttgtt tcgccacatg
    16081 aaagtgatcg aagtctattt gctcaaccac attgggtaag tggatgcacg ttgcccttat
    16141 atagcctaca ttcatgtcca ttgagagtgt tttgatgact aacagctgag ctgtcggtga
    16201 gcatgcgaag ataaccggca agactccctg cacagtggtt gcaaaatggc atctgaatcg
    16261 atcacagtta ggcatgcttc cgatcattga ttggcatcct gtcagtcact ccggaaatta
    16321 actcttttca ccacagtttt cgtccatgac gatgacctct ggtgaattaa tacaccacta
    16381 tcaagtattc agccaccatg ttatgagtaa ccccatataa atgcagcata gaagaagctt
    16441 tgttaatttt gaaatccgga ttcccaaaga gataatgacc tgaatatcca aacagctttg
    16501 taaataattg aaagttacca tagttcttaa atagagagat aacttaccct aagattcgtc
    16561 gaggagtata ataatatttt accaacatat tgacttccac ttaaaaatat cataaaaaag
    16621 cctcccttaa agggattatg gaaattctat agaaaattac tttcattcaa aggaagacaa
    16681 aatgccgaca atccatcatt ctacttcgta taccacagct cctctatcaa aaattaaaga
    16741 aatcagaaca tgggacacaa acaaatactt aatgaaacta tcagaaacta ataacgatat
    16801 agttttt
//
LOCUS       DAZKHC020000089        16598 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZKHC020000089 DAZKHC020000000
VERSION     DAZKHC020000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.089"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1095
                     /gene="emrK"
                     /locus_tag="ACHOSM_RS17825"
                     /old_locus_tag="pgaptmp_003565"
     CDS             <1..1095
                     /gene="emrK"
                     /locus_tag="ACHOSM_RS17825"
                     /old_locus_tag="pgaptmp_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HGU4559997.1"
                     /translation="LFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTV
                     VNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASA
                     RIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKALVMN
                     TPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVVP
                     ARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQ
                     NATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSITATIDTKNEDIAEMPELASTVTS
                     MPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            1095..2633
                     /gene="emrY"
                     /locus_tag="ACHOSM_RS17830"
                     /old_locus_tag="pgaptmp_003566"
     CDS             1095..2633
                     /gene="emrY"
                     /locus_tag="ACHOSM_RS17830"
                     /old_locus_tag="pgaptmp_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HGU4559998.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2741..4069)
                     /gene="dsdA"
                     /locus_tag="ACHOSM_RS17835"
                     /old_locus_tag="pgaptmp_003567"
     CDS             complement(2741..4069)
                     /gene="dsdA"
                     /locus_tag="ACHOSM_RS17835"
                     /old_locus_tag="pgaptmp_003567"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HGU4559999.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHFFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4087..4305)
                     /locus_tag="ACHOSM_RS17840"
                     /old_locus_tag="pgaptmp_003568"
                     /pseudo
     CDS             complement(4087..4305)
                     /locus_tag="ACHOSM_RS17840"
                     /old_locus_tag="pgaptmp_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            4536..5534
                     /locus_tag="ACHOSM_RS17845"
                     /old_locus_tag="pgaptmp_003569"
     CDS             4536..5534
                     /locus_tag="ACHOSM_RS17845"
                     /old_locus_tag="pgaptmp_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4560000.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTIMGLRHITLPESLYGENIVLANCVADDPALPSYIP
                     DDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFH
                     MATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVM
                     GFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            complement(5542..6975)
                     /gene="cscA"
                     /locus_tag="ACHOSM_RS17850"
                     /old_locus_tag="pgaptmp_003570"
     CDS             complement(5542..6975)
                     /gene="cscA"
                     /locus_tag="ACHOSM_RS17850"
                     /old_locus_tag="pgaptmp_003570"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HGU4560001.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLVLRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            7191..8104
                     /locus_tag="ACHOSM_RS17855"
                     /old_locus_tag="pgaptmp_003571"
                     /pseudo
     CDS             7191..8104
                     /locus_tag="ACHOSM_RS17855"
                     /old_locus_tag="pgaptmp_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
     gene            8176..9422
                     /locus_tag="ACHOSM_RS17860"
                     /old_locus_tag="pgaptmp_003572"
                     /pseudo
     CDS             8176..9422
                     /locus_tag="ACHOSM_RS17860"
                     /old_locus_tag="pgaptmp_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
     gene            complement(9951..10151)
                     /gene="torI"
                     /locus_tag="ACHOSM_RS17865"
                     /old_locus_tag="pgaptmp_003573"
     CDS             complement(9951..10151)
                     /gene="torI"
                     /locus_tag="ACHOSM_RS17865"
                     /old_locus_tag="pgaptmp_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HGU4560002.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(10210..10377)
                     /locus_tag="ACHOSM_RS17870"
                     /old_locus_tag="pgaptmp_003574"
     CDS             complement(10210..10377)
                     /locus_tag="ACHOSM_RS17870"
                     /old_locus_tag="pgaptmp_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560003.1"
                     /translation="MKFKVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHAEVTNI
                     RIEELKEHQAA"
     gene            complement(10420..11043)
                     /locus_tag="ACHOSM_RS17875"
                     /old_locus_tag="pgaptmp_003575"
     CDS             complement(10420..11043)
                     /locus_tag="ACHOSM_RS17875"
                     /old_locus_tag="pgaptmp_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HGU4560004.1"
                     /translation="MTSQLIPVFNGTIANETALLCNARDLHAFLGVKKVFAAWITNRI
                     SEYEFIENQDYILLSNLGKQTSGRGGHNRKDYHLTLDTAKELAMVEHNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMEKAQDISKRLYPILKELNSSYASKLYD
                     IAFETFYTVTKNRDALLREATRIDQTSAIFERARPMLKSLRARQFEF"
     gene            complement(11367..12110)
                     /locus_tag="ACHOSM_RS17880"
                     /old_locus_tag="pgaptmp_003576"
     CDS             complement(11367..12110)
                     /locus_tag="ACHOSM_RS17880"
                     /old_locus_tag="pgaptmp_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560005.1"
                     /translation="MINNIANQSSFPAATIDSQMLLKMVNEARRLRGEKEVRNNDFIA
                     RIKDELEGEGYEIFVTPMDKKKGGADQVVIVMTYKQALRVAARESKAVRRSLVDKLES
                     MQQQLQQKTTTKKSPDGLEEFRKARALKMTVDTMKDLFDFLPHLAPEAKQVVAASLIN
                     PVVGFSAIPLPVIDEHHYSASEVGTMLGISANKVGRIANTYMLKTEKYGKWFIDKSAH
                     SDKQVETFRYNEAGVHKIEELIEGERKAA"
     gene            complement(12160..12363)
                     /locus_tag="ACHOSM_RS17885"
                     /old_locus_tag="pgaptmp_003577"
     CDS             complement(12160..12363)
                     /locus_tag="ACHOSM_RS17885"
                     /old_locus_tag="pgaptmp_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560006.1"
                     /translation="MNGQISIVRPGACDDCEIRMIIRLARGKTITALITPENLALALT
                     GKSDLPVELKLRNVEIKVSISNE"
     gene            complement(12365..13135)
                     /locus_tag="ACHOSM_RS17890"
                     /old_locus_tag="pgaptmp_003578"
     CDS             complement(12365..13135)
                     /locus_tag="ACHOSM_RS17890"
                     /old_locus_tag="pgaptmp_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4560007.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLIESDAQAYANAEFIAEANPATVLALLDEQERNQQYIKRRDQEN
                     EDIALTVGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRNAKLQSENAY
                     IRNRYKELDLLIGKNILVMQAAIIEWQATGDAKSGLAWIYNTLFGPGELPDESEKDAQ
                     AYFNRKYAPIDEKLMALHKWFWEQSEAERAAGIRIKGE"
     gene            complement(13132..13353)
                     /locus_tag="ACHOSM_RS17895"
                     /old_locus_tag="pgaptmp_003579"
     CDS             complement(13132..13353)
                     /locus_tag="ACHOSM_RS17895"
                     /old_locus_tag="pgaptmp_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU4560008.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(13452..13667)
                     /locus_tag="ACHOSM_RS17900"
                     /old_locus_tag="pgaptmp_003580"
     CDS             complement(13452..13667)
                     /locus_tag="ACHOSM_RS17900"
                     /old_locus_tag="pgaptmp_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU4560009.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(13744..13935)
                     /locus_tag="ACHOSM_RS17905"
                     /old_locus_tag="pgaptmp_003581"
     CDS             complement(13744..13935)
                     /locus_tag="ACHOSM_RS17905"
                     /old_locus_tag="pgaptmp_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU4560010.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
     gene            complement(13908..14090)
                     /locus_tag="ACHOSM_RS17910"
                     /old_locus_tag="pgaptmp_003582"
     CDS             complement(13908..14090)
                     /locus_tag="ACHOSM_RS17910"
                     /old_locus_tag="pgaptmp_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HGU4560011.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            complement(14090..14767)
                     /locus_tag="ACHOSM_RS17915"
                     /old_locus_tag="pgaptmp_003583"
     CDS             complement(14090..14767)
                     /locus_tag="ACHOSM_RS17915"
                     /old_locus_tag="pgaptmp_003583"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU4560012.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            complement(14764..15549)
                     /gene="bet"
                     /locus_tag="ACHOSM_RS17920"
                     /old_locus_tag="pgaptmp_003584"
     CDS             complement(14764..15549)
                     /gene="bet"
                     /locus_tag="ACHOSM_RS17920"
                     /old_locus_tag="pgaptmp_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGU4560013.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            complement(15555..15851)
                     /locus_tag="ACHOSM_RS17925"
                     /old_locus_tag="pgaptmp_003585"
     CDS             complement(15555..15851)
                     /locus_tag="ACHOSM_RS17925"
                     /old_locus_tag="pgaptmp_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4560014.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            complement(15927..16070)
                     /gene="kil"
                     /locus_tag="ACHOSM_RS17930"
                     /old_locus_tag="pgaptmp_003586"
     CDS             complement(15927..16070)
                     /gene="kil"
                     /locus_tag="ACHOSM_RS17930"
                     /old_locus_tag="pgaptmp_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HGU4560015.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILMLKLRSSK
                     SIH"
     gene            complement(16039..16203)
                     /locus_tag="ACHOSM_RS17935"
                     /old_locus_tag="pgaptmp_003587"
     CDS             complement(16039..16203)
                     /locus_tag="ACHOSM_RS17935"
                     /old_locus_tag="pgaptmp_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HGU4560016.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDIPNQPGVPK
                     NGSNTYGYPD"
     gene            complement(16276..16596)
                     /locus_tag="ACHOSM_RS17940"
                     /old_locus_tag="pgaptmp_003588"
     CDS             complement(16276..16596)
                     /locus_tag="ACHOSM_RS17940"
                     /old_locus_tag="pgaptmp_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HGU4560017.1"
                     /translation="MEIEIDHDVMTEEKLHQINNFWSDSEYRLNKHGSVLNAVLIMLA
                     QHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPSMDGSEGIRITDIDTSGIFDSDDMT
                     IKAA"
BASE COUNT         3961 a         3879 c         3957 g         4801 t
ORIGIN      
        1 ttatttattg cgttttcagg tgcctatgcc tattggtcaa tggaattaga agacatgatt
       61 agtacagatg acgcctatgt cacggggaat gcagatccaa tttctgcaca agtctcaggt
      121 agtgtcactg tcgttaatca taaagatacg aactacgttc gacaaggtga cattttagtt
      181 tcactggata aaactgatgc cactatcgca ctcaataaag ctaaaaataa tctggcaaat
      241 attgttcggc aaacgaataa actatactta caggataaac aatacagtgc cgaagtcgct
      301 tcagcacgta ttcagtatca acaatcttta gaagattata accgtcgagt gccgttagcg
      361 aagcaggggg ttatttcaaa agaaacgctg gagcatacca aagatacgtt aataagtagc
      421 aaagcggcat tgaatgccgc tatccaggct tataaagcga ataaagcttt agtaatgaac
      481 acaccattaa accgtcagcc acaagtcgtt gaagcggcgg atgcaactaa agaagcctgg
      541 ttggcgctta aacgtacgga tattaagagt ccggttaccg gctatattgc ccagagaagt
      601 gttcaggtcg gcgaaacagt gagccccgga caatcgttaa tggctgtcgt accggcacgt
      661 caaatgtggg ttaatgccaa ctttaaagaa acacaactca cggatgtacg gattggtcaa
      721 tcggtcaata ttatcagcga tctttatggt gaaaatgttg tgtttcatgg tcgggtgaca
      781 gggatcaata tgggaaccgg caatgcgttc tccttattac ctgcacaaaa tgcgacaggg
      841 aactggatca aaatcgttca gcgtgtaccg gttgaagttt ctcttgatcc aaaagaactc
      901 atggaacatc ccttgcgtat tggtttatcg ataacagcaa ctattgatac gaagaacgaa
      961 gacattgccg agatgcctga gctggcttca accgtgacct ccatgccggc ttataccagt
     1021 aaggctttag ttatcgatac cagtccgata gaaaaagaaa ttagcaacat tatttcgcat
     1081 aatggacaac tttaatggca atcactaaat caactccggc accattaacc ggtgggacgt
     1141 tatggtgcgt cactattgca ttgtcattag cgacatttat gcaaatgttg gattccacta
     1201 tttctaacgt cgcaataccg acaatatctg gctttctggg agcatcaaca gacgaaggca
     1261 cctgggttat cacctcgttt ggtgtagcaa atgccattgc gatccctgtt actggcaggt
     1321 tggcacaaag aataggcgaa ttaagattat ttttactttc agtaaccttt ttttcgctgt
     1381 cttcattaat gtgtagccta tcgaccaatc ttgatgtgct gatatttttt agagtcgttc
     1441 aggggttaat ggcggggccg ttaattccac tgtcacagag tttattatta aggaattatc
     1501 cgccagaaaa aagaacattt gctctggcat tatggtcaat gaccgtgatt atcgctccga
     1561 tatgtgggcc gatattgggc ggttatattt gtgataactt tagctggggt tggatatttt
     1621 taatcaatgt ccctatgggg attatcgtcc tgacattatg cttaacctta cttaaaggaa
     1681 gagaaactga gacttcaccg gtcaaaatga atctaccagg acttaccctg ttagtgctcg
     1741 gtgttggtgg cttgcaaatt atgcttgata aagggcgcga tctggattgg ttcaactcga
     1801 gtacaataat aatattaaca gtagtatcag ttatttctct gatctcttta gtcatttggg
     1861 agtcgacctc agagaacccg attcttgatc tcagtttgtt taagtcccgt aacttcacca
     1921 ttggtattgt gagtatcaca tgcgcgtatt tattttactc tggagcgatc gtccttatgc
     1981 cgcagttact ccaggaaacg atggggtata atgcgatatg ggccggactt gcttatgcgc
     2041 ccatcggcat catgccacta ttaatttcac ctttgatagg acgttatggc aacaaaatag
     2101 acatgcggtt gttagtgaca tttagttttt tgatgtatgc ggtttgctat tactggcgtt
     2161 ctgtgacatt tatgccaacg attgatttta caggcatcat tttgccgcag ttttttcagg
     2221 gattcgccgt tgcctgtttc tttttaccct taacaacgat ttcgttttca ggcttgccag
     2281 ataataaatt tgccaatgcc tcgagtatga gtaatttttt tcgtaccttg tcaggatcag
     2341 ttggtacgtc gttgacaatg acgctgtggg gacgacgcga atcgttacac catagtcagt
     2401 tgacagcaac catcgatcaa tttaaccccg tgtttaattc atcgtcacaa attatggata
     2461 aatattatgg ttcgctttca ggagttctta atgaaattaa taatgaaata acccagcagt
     2521 cactttctat ttctgcaaat gagattttcc gtatggcggc tattgctttt atcttactta
     2581 cggttttggt ttggtttgcg aaaccgccgt ttacagcgaa aggcgttggg tgatgataaa
     2641 aggagggggt tatagcgggt aagaccgggg atcttaagtc tgcgccatcg gcagcatcgc
     2701 tcacccaggg aaaggattgc gatgctgcgt tgaaacgtta ttaacggcct tttgccagat
     2761 attgattcat ctcttcttcc ggcaccattc cacctcccgt cgcccacacc agatgagtgg
     2821 tattacgcag ttgttctgcg ctgaaaccgt gcatctgttg gtaacttact gatgcacaca
     2881 cgcgctgagg tccggccata cccgccagtg ccgaaggttc aagacgaata ccttcttcct
     2941 gcgccagcca gccaagcatg tcatacatgg tttgatcgct aagggtatag aagccatcca
     3001 gcagacgctc cattgcccgc ccgacaaagc ctgatgcgcg accaactgca aggccatccg
     3061 ctgcggtaag gttgtcgata ccaatatcct gaacagaaat ctgatcgtgt aatcctgtat
     3121 ggacgcctaa caacatacaa ggggagtgcg ttggttcggc aaaaaagaag tgaacatgat
     3181 cgccaaacgc cagtttaagc ccgaatgcga cgccaccagg accaccgcca acaccacacg
     3241 gcagatagac aaacagaggg ttatcagcat cgacgatacg gccttgctgg gcaaattgcg
     3301 ctttaagacg ctggccagcg acggaatacc caaggaacaa cgtgcgggaa ttttcgtcat
     3361 caataaagaa acagttcggg tcagactgcg ctgctttacg tccttcctcg acggcaacac
     3421 cataatcttg ctcatattcc acgaccgtaa cgccatggct gcgcagtttc gcttttttcc
     3481 atgcccgggc atcagcagac atatgaactg tcaccttaaa gccaatgcgg gcgctcataa
     3541 tgccgattga taaccccaga tttccggttg agcccacagc aatgctgtat tggctaaaga
     3601 actgtttaaa ctccggagaa agcagtttgc tgtagtcatc atcaagcgtc agcaaccccg
     3661 cttccagagc cagtttttct gcgtgtgcca ggacttcata aatcccgccg cgtgctttta
     3721 tggagccgga aatgggcaaa tggctatctt ttttcagtaa cagttgcccg ctgatcggtt
     3781 gctgatattc tttttccagc cgtttttgca tagctggaat ggcaaccagt tctgattcaa
     3841 taatcccccc agtggcagca gtttcaggaa atgcttttgc cagatagggt gcaaaacggg
     3901 ataagcgcgc atgggcgtcc tgaacatcct gttcggtcag gccaacataa ggtaaacctt
     3961 cagccaatga ggtcgtgcca ggattaaacc aggtggtttc tttaagagca accagatcct
     4021 ttaccaacgg atactgggcg atgagcgagt tcattttagc gttttccata gtacgtctct
     4081 ttgcgcttag atgataaatg acagcaggaa tgtgcccgcc agagcgacga ctgaagcgat
     4141 aaatgtcgct gtcgtatagt atttaaatgt ttcattgagc gtagcgccgc aatattgctt
     4201 cactagccag aaaagcgagt ccgtaacgat agtacagcca attgcacctg aatcgatagc
     4261 aatcgcaata atttccgggc tgatgtcggg atacagcggc agcacaatga tccatattta
     4321 tattttcttc tccaggttgg cgatgccagt tggcatgttt tacttacttg cctatatggt
     4381 tactcgttgg cgagaacaga ggcggaattg gggacaattg tccgggaatt attgttattt
     4441 tttattgtat tgctgtgtca ggtgataggg cgttggatct cgccagtgac gtctgtttct
     4501 gctacagtgc ccgttttacg gcaaacggct tgggtatggc ttcattaaag gatgtcgcac
     4561 gcctggcggg agtgtcgatg atgacagtct cccgggtgat gcataatgca gaatctgtgc
     4621 gtcctgcaac gcgtgaccgc gtattgcagg caatccagac cctgaattat gttcctgatc
     4681 tttccgcccg taagatgcgc gctcaaggac gtaagccgtc gactctcgcc gtgctggcgc
     4741 aggacacggc taccactcct ttctctgttg atattctgct tgccattgag caaaccgcca
     4801 gcgagttcgg ctggaatagt tttttaatca atattttttc tgaagatgac gctgcccgcg
     4861 cggcacgtca gctgcttgcc caccgtccgg atggcattat ctatactaca ataatggggc
     4921 tgcgacatat cacgctgcct gagtccctgt atggtgaaaa tattgtattg gcgaactgtg
     4981 tggcggatga cccagcgtta cccagttata tccctgatga ttacactgca caatatgaat
     5041 caacacagca tttgctcgcg gcgggctatc gtcaaccgtt atgcttctgg ctaccggaaa
     5101 gtgcgttggc aacagggtat cgtcggcagg gatttgagca ggcctggcgt gatgctggac
     5161 gagatctggc tgaggtgaaa caatttcaca tggcaacagg tgatgatcac tacaccgatc
     5221 tcgcaagttt actcaatgcc cacttcaaat cgggcaaacc agattttgat gttctgatat
     5281 gtggtaacga tcgcgcagcc tttgtcgctt atcaggttct cctggcgaag ggggtacgta
     5341 tcccgcagga tgtcgccgta atgggctttg ataatctggt tggcgtcggg catctgtttt
     5401 taccgccgct gaccacaatt cagcttccac atgacattat cgggcgggaa gctgcattgc
     5461 atattattga aggtcgtgaa gggggaagag tgacgcggat cccttgcccg ctgttgatcc
     5521 gttgttccac ctgatattat gttaacccag tagccagagt gctccatgtt gcagcacagc
     5581 cactccgtgg gaggcataaa gcgacagttc ccgttcttct ggctgcggat agattcgact
     5641 actcatcacc gcttccccgt cgttaataaa tacttccacg gatgatgtat cgataaatat
     5701 ccttaggacg agcgtgtcac gctgcgggag gggaatacta cggtagccgt ctaaattctc
     5761 gtgtgggtaa taccgccaca aaacaagtcg ctcagattgg ttatcaatat acagccgcat
     5821 tccagtgccg agctgtaatc cgtaatgttc ggcatcactg ttcttcagcg cccactgcaa
     5881 ctgaatctca actgcttgcg cgttttcctg caaaacatat ttattgctga ttgtgcgggg
     5941 agagacagat tgatgctgct ggcgtaacga ctcagcttcg tgaaccgggc gttgtagaag
     6001 tttgccattg ctctctgata gctcgcgcgc cagcgtcatg cagcctgccc atccttcacg
     6061 ttttgagggc attggcgatt cccacatatc catccagccg ataacaatac gccgaccatc
     6121 cttcgctaaa aagctttgtg gtgcataaaa gtcatgcccg ttatcaagtt cagtaaaatg
     6181 cccggattgt gcaaaaagtc gtcctggcga ccacattccg ggtattacgc cactttgaaa
     6241 gcgatttcgg taactgtatc cctcggcatt cattccctgt ggggaaaaca tcagataatg
     6301 ctgatcgcca aggctgaaaa agtccggaca ttcccacata tagctttcac ccgcatcagc
     6361 gtgggccagt acgcgatcga aggtccattc acgcaatgaa ctgccgcgat aaagcaggat
     6421 ctgccccgtg ttgcctggat ctttcgcccc gactaccatc caccatgtgt cggcttcacg
     6481 ccacacttta ggatcgcgga agtgcatgat tccttctggt ggagtgagga tcacaccctg
     6541 tttctcgaaa tgaataccat cccgactggt agccagacat tgtacttcgc gaattgcatc
     6601 gtcattacct gcaccatcga gccagacgtg tccggtgtag ataagtgaga ggacaccatt
     6661 gtcatcgaca gcactacctg aaaaacaccc gtctttgtca ttatcgtctc ctggcgctag
     6721 cgcaataggc tcatgctgcc agtggatcat atcgtcgctg gtggcatgtc cccagtgcat
     6781 tggcccccag tgttcgctca tcgggtgatg ttgataaaac gcgtgataac gatcgttaaa
     6841 ccagatcagg ccgtttggat cgttcatcca cccggcagga ggcgcgaggt gaaaatgggg
     6901 atagaaagtg ttaccccggt gctcatgaag ttttgctagg gcgttttgcg ccgcatgcaa
     6961 tcgagattgc gtcattttaa tcatcctggt taagcaaatt tggtgaattg ttaacgttaa
     7021 cttttataaa aataaagtcc cttactttca taaatgcgat gaataccaca aatgttaacg
     7081 ttaactatga cgttttgtga tcgaatatgc atgttttagt aaatccatga cgattttgcg
     7141 aaaaagaggt ttatcactat gcgtaactca gatgaattta agggaaaaaa atgtcagcca
     7201 aagtatgggt tttaggggat gcggtcgtag atctcttgcc agaatcagac gggcggctac
     7261 tgccttgtcc tggcggcgcg ccagctaacg ttgcggtggg aatcgccaga ttaggcggaa
     7321 caagtgggtt tataggtcgg gtgggggatg atccttttgg tgcgttaatg caaagaacgc
     7381 tgctaactga gggagtcgat atcacgtatc tgaagcaaga tgaatggcac cggacatcca
     7441 cggtgcttgt cgatctgaac gatcaagggg aacgttcatt tacgtttatg gtccgcccca
     7501 gtgccgatct tttttagaga cgacagactt gccctgctgg cgacatggcg aatggttaca
     7561 tctctgttca attgcgttgt ctgccgagcc ttcgcgtacc agcgcattta ctgcgatgac
     7621 ggcgatccgg catgccggag gttttgtcag cttcgatcct aatattcgtg aagatctatg
     7681 gcaagacgag catttgctcc gcttgtgttt gcggcaggcg ctacaactgg cggatgtcgt
     7741 caagctctcg gaagaagaat ggcgacttat cagtggaaaa acacagaacg atcaggatat
     7801 atgcgccctg gcaaaagagt atgagatcgt catgctgttg gtgactaaag gtgcagaagg
     7861 ggtggtggtc tgttatcgag gacaagttca ccattttgct ggaatgtctg tgaattgtgt
     7921 cgatagcacg ggggcgggag atgcgttcgt tgccgggtta ctcacaggtc tgtcctctac
     7981 gggattatct acagatgaga gagaaatgcg acgaattatc gatctcgctc aacgttgcgg
     8041 agcgcttgca gtaacggcga aaggggcaat gacagcgctg ccatgtcgac aagaactgga
     8101 atagtgagaa gtaaacggcg aagtcgctct tatctctaaa taggacgtga attttttaac
     8161 gacaggttgg taattatggc actgaatatt ccattcagaa atgcgtacta tcgttttgca
     8221 tccagttact catttctctt ttttatttcc tggtcgctgt ggtggtcgtt atacgctatt
     8281 tggctgaaag gacatctagg gttgacaggg acggaattag gtacacttta ttcggtcaac
     8341 cagtttacca gcattctatt tatgatgttc tacggcatcg ttcaggataa actcggtctg
     8401 aagaaaccgc tcatctggtg tatgagtttc atcctggtct tgaccggacc gtttatgatt
     8461 tacgtttatg aaccgttact gcaaagcaat ttttctgtag gtctaattct gggggcgctc
     8521 ttttttggcc tggggtatct ggcgggatgc ggtttgcttg acagcttcac cgaaaaaatg
     8581 gcgcgaaatt ttcatttcga atatggaaca gcgcgcgcct ggggatcttt tggctatgct
     8641 attggcgcgt tctttgccgg catatttttt agtatcagtc cccatatcaa cttctggttg
     8701 gtctcgctat ttggcgctgt atttatgatg atcaacatgt gttttaaaga taaggatcac
     8761 cagtgcgtag cggcggatgc gggaggggta aaaaagagga ttttatcgca gttttcaagg
     8821 atcgaaactt ctgggttttc gtcatattta ttgtggggac gtggtctttc tataacattt
     8881 ttgatcaaca actttttcct gtcttttatg caggtttatt cgaatcacac gatgtaggaa
     8941 cgcgcctgta tggttatctc aactcattcc aggtggtact cgaagcgcta tgcatggcga
     9001 ttattccttt ctttgtgaat cgggtagggc caaaaaatgc attacttatc ggtgttgtga
     9061 ttatggcgtt gcgtatcctt tcctgcgcgc tgttcgttaa cccttggatt atttcattag
     9121 tgaagctgtt acatgccatt gaggttccac tttgtgtcat atccgtcttc aaatacagcg
     9181 tggcaaactt tgataagcgc ctgtcgtcga cgatctttct gattggtttt caaattgcca
     9241 gttcgcttgg gattgtgctg ctttcaacgc cgactgggat actctttgac cacgcaggct
     9301 accagacagt tttcttcgca atttcgggta ttgtctgcct gatgttgcta tttggcattt
     9361 tcttcctgag taaaaaacgc gagcaaatag ttatggaaac gcctgtacct tcagcaatat
     9421 agacgtaaac ttttccggtt gttgtcgata tctccctatc cctcaaccgg aaaataataa
     9481 tactaaagtg cttagccctg ctaataatca cctaatccaa acgcctcatt catgttctgg
     9541 tacagtcgct caaatgtact tcagatgcgc ggttcgctga tttccaggac attgtcgtca
     9601 ttcagcgacc tgtcccgtgt atcacggtcc tgcgaattca tcaaggaatg cattgcggag
     9661 tgaagtatcg agtcacgcca tatttcgcta tcaggattct gtgtgatggt tacatcgccc
     9721 ggcccagggc tgtttagtca tcagcgcttt ctgacagtgc tgagatttca acctgttgca
     9781 gtaaaaatga gtagatataa ggcaagtgtg ctgccaaacc catcttttac ggggtgaagg
     9841 tagatttcgt ttgaagggta tctggtgtcc cctgcagaca tctacttgag gcagcagggg
     9901 attgattgga atggtgtttt ttagatgtga gaaatatttt acccgctatt ttacccattg
     9961 gcgcggctta agagtttatt tttgaactca caatggtcac gatataacca tcttgctcgc
    10021 ccgtggataa ctttggcttt tggcaggtcg ccggacttaa tccggtcata gatgaaggtt
    10081 ttaccaaagc cagtatcagc catgatgaat ttcaaatcaa ccagtgaatc aggttgtagt
    10141 tcgtgttgca tgagtgctat ctccgaatat ggaatcgaac ctgcaaatca ggtaataaaa
    10201 aaaccgccat caggcggctt ggtgttcttt cagttcttca attcgaatat tggttacttc
    10261 tgcatgtgct atctgcgccc atatcatcca gtggtcatag cagtcattga tattctccgc
    10321 ttcgataact ctgttgaatg gttctccatt ccattcacct gtgactttga atttcattaa
    10381 gtttcacctg taaaaaaagg agccgaagct cctttgatat taaaattcga attgtctcgc
    10441 ccgaaggctt tttaacattg gtcttgcccg ttcgaaaatg gcgcttgtct ggtcaattcg
    10501 tgttgcctcc ctgagcaatg cgtctctgtt tttcgtcacc gtgtagaaag tctcaaacgc
    10561 gatgtcatac agcttgctcg cgtatgatga gttcagttcc ttcaatatcg gatacaggcg
    10621 tttgctgatg tcctgtgctt tctccatctg tacctgcatg tagcagagga ggatgatttc
    10681 ctcgtctgtg aattgctgtg ctggttgcat gctgcgaagt ttcttttcgc actcgatgaa
    10741 gtatcggcgt atctggcggc ctttttcgtt gtgctccacc atcgccagtt ctttggctgt
    10801 gtcgagggtg aggtggtaat ccttgcggtt gtgaccgcct ctaccagatg tttgctttcc
    10861 caaattggaa agcaaaatat agtcttgatt ttcaatgaat tcgtattctg aaatgcgatt
    10921 tgtaatccat gccgcaaaca cctttttcac gcctaaaaaa gcatgcagat cgcgggcatt
    10981 gcagagtagg gctgtttcgt tggcgatagt gccgttgaat acggggatga gttgactggt
    11041 catttttatg tcctttgcat ttgttcagat agccctgtgt tcagcagggc ggtcaggtac
    11101 ttgaacaccg tgcaaagtcg gcccgtatcc ttaacctttc ggttgttttt cggtatacgc
    11161 gctacccgac catatctgag aaatggacat aaaaaagccg catgactgac gggtgcggtt
    11221 tccgctttgc aaggtgtgtt cagcaccatg aagcggaata tagccccgtt aatgcggatt
    11281 tgtcaaatca tgtggtcttt atcttgctgt aagccgcgcc attcggtctt ttccccccag
    11341 atttggggaa aactatcttg tcaaaatcat gcagcctttc gttctccttc gatgagttcc
    11401 tcgatcttgt gcactcccgc ctcgttgtag cgaaatgtct cgacctgctt gtcactgtgc
    11461 gccgatttgt cgatgaacca cttcccgtac ttttctgttt tgagcatgta ggtgttggca
    11521 atgcgcccta ccttgttggc tgaaatgcca agcatggtgc caacctccga cgcagagtag
    11581 tgatgttcat caatgaccgg aagagggatt gcactaaagc cgacgacggg attaatcagg
    11641 ctggcggcaa cgacctgttt agcttccggt gcgaggtgag ggaggaagtc gaagaggtct
    11701 ttcattgtgt cgacggtcat tttcaatgcg cgtgccttac ggaattcttc aaggccatcc
    11761 ggtgatttct tcgtggtcgt tttttgttgc aactgctgtt gcattgattc aagtttgtcg
    11821 accagcgaac gacggacggc tttcgactca cgtgcggcga cgcgcagggc ttgcttgtag
    11881 gtcatcacta taaccacctg atccgctccg ccttttttct tatccatggg ggttacgaaa
    11941 atttcgtaac cctccccctc aagctcatct ttgatgcgtg caatgaagtc gttgttgcgt
    12001 acttcttttt cgcctcgcag tctccgcgcc tcattaacca ttttcaacag catctggctg
    12061 tcaattgttg cagccggaaa cgaagactga tttgcaatat tgttaatcat gtattctgtc
    12121 cttttcagat aaaagaaatc cccgccaatg cgaggattgt tattcattgc tgatactcac
    12181 cttaatctca acatttcgca gctttagctc tactggcagg tctgactttc cggttaatgc
    12241 taatgcgaga ttttctggag taatgagagc agttattgtt ttccccctcg ccagacgaat
    12301 aatcattcgt atctcgcaat cgtcacatgc tcccggtcga acaattgaga tttgtccgtt
    12361 catctcactc tcctttgatg cgaatgccag cggcgcgctc ggcttcactt tgttcccaaa
    12421 accacttgtg aagcgccata agcttttcgt caatcggtgc atatttgcga ttaaagtagg
    12481 cctgagcatc tttctcagat tcgtccggta attcgccagg gccaaacagt gtgttataaa
    12541 tccatgctag tccgctctta gcgtcgccag ttgcctgcca ttcgataatg gcagcctgca
    12601 tgaccagaat gtttttcccg attaataggt ccagttcttt gtaccggttg cggatgtatg
    12661 cattctcgct ttgtaatttt gcgttgcgct tttctgaggc ttcaagtaac gcctgcttat
    12721 cgcgtagcgc ttcttccagt tcagcaacat ggcattcgct atcaataagg ttgttctctg
    12781 ctgcttcaag ctcaacacgc agcttcccta ccgttagcgc aatatcctcg ttctcctggt
    12841 cacggcgttt gatgtattgc tggtttcttt cctgttcatc cagcagtgcc agcacggtag
    12901 ccgggttagc ctctgctatg aattcagcgt ttgcataagc ctgagcatct gattcaatca
    12961 ggcagttaac atgacattcc gcaatcacgc caccgggttc tcctttccat ttttggcaaa
    13021 caaaaactcc tgttaaattg ccgtgctggt taacagatgt atgccctacg atgtagcttc
    13081 ctttagttgc tttctctgcc ttttcacgca gtgcctgata attaatttcg ctcacttcga
    13141 acctctctgt ttactgataa gctccagatc ctcctggcaa cttgcacaag tccgacaacc
    13201 ctgaactgcc aggcgtcttc gttcatctat cggatcgcca cactcacaac aatgagttgc
    13261 ggatacagtc tggtagttca ggcgacgcat ttttattgct gtattgcgct gtaattcttc
    13321 aatttctgat gctgaatcaa tgatatctgc catctttcat taatccctga attgttggtt
    13381 aatacgcttg agggtgaatg cgaacaataa aaaaggagcc tgtagctccc tgatgatttt
    13441 gcttttcatg ttcatcgctc cttaaagacg ccgtttaaca tgccgatcgc cagacttaaa
    13501 tgagtcggtg tgaatcccat tagcgttacc gtttcgcggt gcttcttcag tacgctacgg
    13561 caaatgtcat cgacgttttt atccggaaac tgctgtctgg cttttttgat ttcagaatta
    13621 gcctgacggg caatgctgcg aagggcgttt tcctgctgag gtgtcattga acaagtccca
    13681 tgtcggcaag cataagcaca cagaatatga agcctgctgc cagaaaaatg cattctgttg
    13741 ttgtcatgcc gggtctctct cgtttgcttc tgctttcgcc gccatcattt ccagcttttg
    13801 tgaaagggat gtggctaacg tatgaaattc ttcgtctgtt tctactggta ttggcacaaa
    13861 cctgactcca atttgagcga ggctatgtgc catcccgata ctcgttctta attcaacagg
    13921 agatgctttg tgcatacagc ccctcgttta ttatttatct cttcagccag ccgctgtgct
    13981 ttcagtggat ttcggataac agaaaggccg ggaaataccc agcctcgctt tgtaacggag
    14041 tagacgaaag tgatcgtgcc tacccggata ttatcgtgag gatgcttcat taccattgct
    14101 ccccatatac aaaaccaatt tcagccagtg cctcgtccat tttttcgatg aactccggca
    14161 ccatctcgtc aaaactcgcc atgtactttt cattccgctc aatcacgaca taatgcaggc
    14221 cttcacgctt catgcgcggg tcatagttgg caaagtacca ggcatctttt cgtgtcaccc
    14281 acatgctgta ctgcacctgg gccatgtaag ccgattttat ggcctcgaaa ccaccgagcc
    14341 ggaacttcat gaaatcccgg gaggtaaacg ggcatttcag ctcaaggccg ttgccgtcac
    14401 tgcataaacc atcgggagag caggcggtgc gcatactttc gtcgcgatag atgatcgggg
    14461 attcagtaac attcacgccg gaagtgaact caaagagggt tctggcgtcg ttctcgtact
    14521 gttttcccca ggccagagcc ttagcgttaa cttccggagc cacaccggtg caaacctcag
    14581 ccagcagggt gtggaagtag gacattttca tgtcaggcca cttctttcct gatcggggtt
    14641 ttgctattac gttgtgaatt tctgaagctg tgatgacgcc gagccgtaat ttgtgccacg
    14701 catcatctcc ctgttcgaca gctctcacgt cgatcccagt acgctgcagg ataatgtccg
    14761 gtgtcatgct gccaccttct gttcagtggc tttttgtttc aggaatccaa gagcttttac
    14821 tgcttcggcc tgtgtcagtt ctgacgatgc acgaatgtcg cggcgaaata tctgggaaca
    14881 gagcggcaat aagtcgtcat cccatgtttt atccagggcg atcagcagag tgttaatttc
    14941 ctgcatggtt tcatcgttaa ccggagtgat gtcgcgttcc ggctgacgtt ctgcagtgta
    15001 tgcggtattt tcgacaatgc gctcggcttc atccttgtca tagataccag caaatccgaa
    15061 ggcgagacgg gcacactgaa tcatggcttt atgacgtaac atccgtttgg gatgcgactg
    15121 ccacggcccc gtgatttctc tgccttcgcg ggttttgaat ggttcgcggc ggcattcatc
    15181 catccactcg gtaacgcaga tcggatgatt acggtccttg cggtaaatcc ggcatgtgca
    15241 ggattcattg tcctgctcaa agtccatgcc atcaaactgc tggttttcgt tgatgatgcg
    15301 ggaccagcca tcaacgccca ccaccggaac gatgccgttc tgcttatcag ggaaggcgta
    15361 aatttctttc gtccacggat taaggccgta ctggttggcg acgatcagca atgcgatgaa
    15421 ctgcgcatcg ctggcatcac ctttaaatgc cgtctggcga agagtggtga tcagttcctg
    15481 tgggtcgaca gaatccatgc cgacacgttc agccagcttc ccagccagcg ttgcgagtgc
    15541 tgtactcatc cgttttatac ctctgaatca atatcaacct ggtggtgagc aatggtttca
    15601 accatgtacc ggatgtgttc tgccatgcgt tcctgaaact caacatcgtc atcaaacgca
    15661 cgggtaatgg cttttttgct ggccccgtgg cgttgcaaat gatcgatgca tagcgattca
    15721 aacaggtgct ggggcaggcc tttttccatg tcgtctgcca gttctgcctc tttctcttca
    15781 cgggcgatct gctggtagtg acgcgcccag ctctgagcct caagacgatc ctgaatgtaa
    15841 taagcgttca tggctgaact cctgaaaatg gctgtgaaaa tatcgcccgc gaaatgccag
    15901 gctgattagg aaaacaggaa atggggttag tgaatgcttt tgcttgatct cagtttcagc
    15961 attaatatcc attttttata agcgtcgaca gcttcacgaa acatcttttc atcgccaata
    16021 aaagtggcga tagtgaattt agtctggata gccataagtg tttgatccat tctttgggac
    16081 tcctggctga ttaggtatgt cgataaggcg tttccatccg tcacgtaatt tacgggtgat
    16141 tcgttcaagt aaagattcgg aagggcagcc agcaacaggc caccctgcaa tggcatattg
    16201 catggtgtgc tccttattta tacataacga aaaacgcctc gagtgaagcg ttattggtat
    16261 gcggtaaagc cgcactcagg cggccttgat agtcatatca tctgaatcaa atattcctga
    16321 tgtatcgata tcggtaattc ttattccttc gctaccatcc attgaaggcc atccttcctg
    16381 accatttcca tcattccagt cgaactcaca cacaacacca tatgcattta agtcgcttga
    16441 aattgctata agcagagcat gttgcgccag catgattaat acagcattta atacagagcc
    16501 gtgtttattg agtcggtatt cagagtctga ccagaaatta ttaatctggt gaagtttttc
    16561 ctctgtcatt acgtcatggt cgatttcaat ttctattg
//
LOCUS       DAZKHC020000090        16211 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZKHC020000090 DAZKHC020000000
VERSION     DAZKHC020000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.090"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(575..1771)
                     /gene="hemY"
                     /locus_tag="ACHOSM_RS17945"
                     /old_locus_tag="pgaptmp_003589"
     CDS             complement(575..1771)
                     /gene="hemY"
                     /locus_tag="ACHOSM_RS17945"
                     /old_locus_tag="pgaptmp_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HGU4560018.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHVTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1774..2985)
                     /gene="hemX"
                     /locus_tag="ACHOSM_RS17950"
                     /old_locus_tag="pgaptmp_003590"
     CDS             complement(1774..2985)
                     /gene="hemX"
                     /locus_tag="ACHOSM_RS17950"
                     /old_locus_tag="pgaptmp_003590"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HGU4560019.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPVPQ
                     ADTPAAAPQGE"
     gene            complement(3007..3747)
                     /gene="hemD"
                     /locus_tag="ACHOSM_RS17955"
                     /old_locus_tag="pgaptmp_003591"
     CDS             complement(3007..3747)
                     /gene="hemD"
                     /locus_tag="ACHOSM_RS17955"
                     /old_locus_tag="pgaptmp_003591"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HGU4560020.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3744..4685)
                     /gene="hemC"
                     /locus_tag="ACHOSM_RS17960"
                     /old_locus_tag="pgaptmp_003592"
     CDS             complement(3744..4685)
                     /gene="hemC"
                     /locus_tag="ACHOSM_RS17960"
                     /old_locus_tag="pgaptmp_003592"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HGU4560021.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            5072..7618
                     /gene="cyaA"
                     /locus_tag="ACHOSM_RS17965"
                     /old_locus_tag="pgaptmp_003593"
     CDS             5072..7618
                     /gene="cyaA"
                     /locus_tag="ACHOSM_RS17965"
                     /old_locus_tag="pgaptmp_003593"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HGU4560022.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7658..7978)
                     /gene="cyaY"
                     /locus_tag="ACHOSM_RS17970"
                     /old_locus_tag="pgaptmp_003594"
     CDS             complement(7658..7978)
                     /gene="cyaY"
                     /locus_tag="ACHOSM_RS17970"
                     /old_locus_tag="pgaptmp_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HGU4560023.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8140..8282)
                     /locus_tag="ACHOSM_RS17975"
                     /old_locus_tag="pgaptmp_003595"
                     /pseudo
     CDS             complement(8140..8282)
                     /locus_tag="ACHOSM_RS17975"
                     /old_locus_tag="pgaptmp_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8441..8644
                     /gene="yifL"
                     /locus_tag="ACHOSM_RS17980"
                     /old_locus_tag="pgaptmp_003596"
     CDS             8441..8644
                     /gene="yifL"
                     /locus_tag="ACHOSM_RS17980"
                     /old_locus_tag="pgaptmp_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4560024.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8681..9505
                     /gene="dapF"
                     /locus_tag="ACHOSM_RS17985"
                     /old_locus_tag="pgaptmp_003597"
     CDS             8681..9505
                     /gene="dapF"
                     /locus_tag="ACHOSM_RS17985"
                     /old_locus_tag="pgaptmp_003597"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HGU4560025.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDLRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9502..10209
                     /gene="yigA"
                     /locus_tag="ACHOSM_RS17990"
                     /old_locus_tag="pgaptmp_003598"
     CDS             9502..10209
                     /gene="yigA"
                     /locus_tag="ACHOSM_RS17990"
                     /old_locus_tag="pgaptmp_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HGU4560026.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMFPELLERWIERV"
     gene            10206..11102
                     /gene="xerC"
                     /locus_tag="ACHOSM_RS17995"
                     /old_locus_tag="pgaptmp_003599"
     CDS             10206..11102
                     /gene="xerC"
                     /locus_tag="ACHOSM_RS17995"
                     /old_locus_tag="pgaptmp_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HGU4560027.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            11102..11818
                     /gene="yigB"
                     /locus_tag="ACHOSM_RS18000"
                     /old_locus_tag="pgaptmp_003600"
     CDS             11102..11818
                     /gene="yigB"
                     /locus_tag="ACHOSM_RS18000"
                     /old_locus_tag="pgaptmp_003600"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HGU4560028.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11902..14064
                     /gene="uvrD"
                     /locus_tag="ACHOSM_RS18005"
                     /old_locus_tag="pgaptmp_003601"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11902..14064
                     /gene="uvrD"
                     /locus_tag="ACHOSM_RS18005"
                     /old_locus_tag="pgaptmp_003601"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HGU4560029.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGVLAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(14211..14975)
                     /gene="yigE"
                     /locus_tag="ACHOSM_RS18010"
                     /old_locus_tag="pgaptmp_003602"
     CDS             complement(14211..14975)
                     /gene="yigE"
                     /locus_tag="ACHOSM_RS18010"
                     /old_locus_tag="pgaptmp_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HGU4560030.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFAVQSGP
                     MLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            15137..15193
                     /gene="ysgD"
                     /locus_tag="ACHOSM_RS18015"
                     /old_locus_tag="pgaptmp_003603"
     CDS             15137..15193
                     /gene="ysgD"
                     /locus_tag="ACHOSM_RS18015"
                     /old_locus_tag="pgaptmp_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HGU4560031.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            15345..>16211
                     /gene="corA"
                     /locus_tag="ACHOSM_RS18020"
                     /old_locus_tag="pgaptmp_003604"
     CDS             15345..>16211
                     /gene="corA"
                     /locus_tag="ACHOSM_RS18020"
                     /old_locus_tag="pgaptmp_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HGU4560032.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWS"
BASE COUNT         3703 a         4215 c         4361 g         3932 t
ORIGIN      
        1 aataaattat ttatttatcc agaaaatgaa ttggaaaatc aggagagcgt tttcaatcct
       61 acctctggcg cagttgatat gtaaggcagg tttattatat cgcgttgatt attgatgctt
      121 tttttagttt taacggcaat taatatatgt gttattaatt gaatgaattt tatcattcat
      181 aataagtatg tgtaggatca agctcaggtt aaatattcac tcaggaagtt attactcagg
      241 aagcaaagag gattacagaa ttatctcata acaagtgtta agggatgtta tttcccgatt
      301 ctctgtggca taataaacga gtagatgctc attccatctc ttatgttcgc cttagtgcct
      361 cataaactcc ggaatgacgc agagccgttt acggtgctta tcgtccactg acagatgtcg
      421 cttatgcctc atcagacacc atggacacaa cgttgagtga agcacccact tgttgtcata
      481 cagacctgtt ttaacgcctg ctccgtaata agagcaggcg tttttttatg catcagaaag
      541 gtcccggagg tgcttgcctc cgggtgagaa ggaactactg tggtgggttg ttctgcaacg
      601 ttaacatcaa accgtcgcga cgcatcgctg cagcttcttc cggcttgtgc agtctgtcca
      661 gcgcgtcggc aagccatgcg taatcgtagg cgtccggacg ttgtttcagc gctgcgcgga
      721 aggcgagcga tgcttcctgc cattctccgt gcttcatcag tgactggccc agtgtgctcc
      781 acaacagcgg gcgatcgccg acgtttttga tctgctggcg cagcactttt tccagctgtt
      841 ccggattgtt ggttttcagt cgaggaatcg gcagcagtag gcgatcgtcg tactggcgtt
      901 tcaggccatc gatgataatt tgctgggcag taacatgatc gtcacattca ataagatgtt
      961 ccgccattgc cacctgcaac gctacctgat gacgcgtttt ccggctttgg tttttccacc
     1021 agttacgcaa accttcacta ccgttatcgg cacgcgcctg atccatcagg ccaatccatg
     1081 cctgttgttc cagcattgca cgatgttctt catcaccaac atgggctttc gccattgatg
     1141 ggatgatatc cagcagagaa ctccatgcac ctgtgcggat atacgcctgt tccgccagac
     1201 gtaatacttc cggatggcgt ggcgtaactt ccagcagctt atccacgccg tggcgtgcag
     1261 catggttttc attacgggcc agttgcagac gtacacgggt gatttctacc ggaatggtgt
     1321 cattgccggc cagctctgct gcgcgttcca gatgttggtt ggcgcgtgct tcatcaccac
     1381 gttgttgcgc ggcttcggca gccagtagat agttcaccac cggttgttca gcgtgatcgg
     1441 catttttcgc catcagcttt tcaacttgct gataatcgcc ttccgccagt ttcagcagcg
     1501 cctgttcggt ctgcttacgg gcacggcgac gcttacgtcc gacaaaccac ccacgggtgt
     1561 gtgcgcctgt gcggaagatc cgccgcagta gccactcaat ggcaaacagt actaccatcg
     1621 ccagaatcaa aataatcgcg aggcccgtga cgctggtttc gatattgtag ttgtcggtct
     1681 ggatcagcac ataaccctga tgaccggcaa tcatcgggcc aaccacgatc cccgcaatca
     1741 gcaacacaaa gagcaataac acttttagca tggttattct ccttgcggcg cggctgccgg
     1801 agtatcagct tgcggtacag gtgcaggtgc aggtgcaggt gcaggtgcag gtgcaggttt
     1861 agcttccgtt gtccccgctg ccggttgtgc cagcaggtta cgcacgcggg tctgcatcag
     1921 tttttccagc atcgcctggc tttgcagggt ttccggaaga tccatcgaga tattttgctg
     1981 gcttaactgg tccacctcgt cgaggaacgc tttggtggtg gcatcatcag tatcgtagta
     2041 agcacgtacc caagtggaga cgttctccag cgcctggcga taagtctctt cctgatgacg
     2101 cggtacagct tgtgctgcga ccagcaggcg agagcgaata ttttcgcgca gatagatatc
     2161 ctgatttggc gctaacagcg gtacggcggt gtcatcacga cggcgaatcg taatgaagtt
     2221 gtccataaag ttctgccagc ttttttgcag attgatacgc cattcgctga tggaactgga
     2281 aagctcttca ccgtctgaat ccatcggcga accatcgctg tcattatcgg ccagacgcag
     2341 gttatctacc tgatttgaaa gctgattaag cttaaggatg atgccgtcat aatccacctg
     2401 cgatactgca gaaaggctgg cgatatcatc ggtaattgcc cgacgaacgg taatcagact
     2461 cgggtcattc atatccgcca ggctggcgtc tgcacttttc agcaacgctg cagcggtcgt
     2521 gacgtcctga tcgctccaca gcttccgtcc ggcgagtttc accagaaaat cggcctgagc
     2581 cagcagccag gttttagcat cgctgccgga aatggtggcg accttttgtt ggacttcatc
     2641 caactgtttt gccagcgttt cttgctgacg attcgcctgc ttaagttgtg cagcttgttg
     2701 cttaataatg ccttccagct cggctttttg gctctcctgg gctttttgca atgccgtcag
     2761 ttggttagcc agggcatcgc tggtggcggt ctgattgacg gcctgttgtt taccccagcc
     2821 atacaaaccg acacccgccg ccagagcaat agcgatagcc accgcgctga gaatcaatgc
     2881 agtattgttc ttactctttt tttctgttgc gacaggttgt gacgtggtgt ccacggcctc
     2941 cctggtctct tcaaccacgg cggaggtttt ttcttgttcc gtcattatgg cttcctgtta
     3001 tgagagttat tgtaatgccc gtaaaagcgc atcgttgtca gcgttatcgg cgaccttaat
     3061 gtcttgccag cccagttccc gggcgagttt cgccaaacgc tcactgacga ccaatagtcg
     3121 acagtgtagt aaccagtgct cacgatacca ttgtgggatc agcgaccaga gttgctgcaa
     3181 catttcaccg ctggtaacaa cgaccatcgt cacctcgcgg gattgccagc gcatcgcttc
     3241 ttctgcacca tcgtaatgga ttgcgcatcg ttgataacat tcacaaaaag tgacctcagc
     3301 accgcgcacc gtcagggtat ccccaattag ctcacgaccg ccattgccac gtaatatcag
     3361 cgcacgtttg cccgcaatat tttgtaattc aggtaattgt agcaagactt cgctgatttc
     3421 ccgatcctgt gggtagagaa tcttctgtcc gcttacggta tgtagcgcca gtgcggtggt
     3481 gcgtccaatg gcgaaataag taggtagttg gggccattta cgatcttgct gatgcagctg
     3541 tgattgggca aaagcaaccg cgtgttgcga gagggcaaac aacagatcgc tctcccccag
     3601 cgctgccagt tgatcagcaa gttgcggtaa ttgtcgaccc ggagaaaact caatcagcgg
     3661 aaaatgccag gccacctgcc ccagtgtgcg cagacggctc actaactctt ctccagcggg
     3721 agacgggcgg gtgacaagga tactcatgcc ggggcgtctc cgttatagac ttcagcgagg
     3781 atctcgcgcg cgccgttatt cagtagctct tctgccagcg aaatccccat ttgttcggca
     3841 ttttgcggcg caccgcggcg ttcaccgcga ataatctgcg aaccgtccgg cgcgccgacc
     3901 agcgcacgca gccagatttc gccatcaata agctcggcgt agctaccaat tggcacctga
     3961 catccgcctt cgagacgggt attcatggcg cgttctgcgg taacgcgcag tgcagtttcg
     4021 tggtgattca gcgcggcaag cagctcgcga gtgcgcgtat catcaaggcg gcattcaata
     4081 ccgactgcac cttgtcctac cgccggaaga gaaatctcgg gtggtaacgc ggcgcgaatg
     4141 cgggactcca gacctaaacg ttttagtccg gctacggcaa gaatgatggc atcgtattcg
     4201 ccgttatcta gtttgctcag gcgagtgccg acgttgccgc gcagggagcg gataatcaga
     4261 tccgggcggc gttcagccag ttggcactgg cgacgtaaac tggacgtccc gacgatactg
     4321 cctgctggta acgcatccag actgtcatag ttattggaca caaaggcatc gcgaggatct
     4381 tcacgctcac aaatagtgac cagtcccaga ccttgcggga attcaaccgg cacatctttc
     4441 attgaatgta cggcgatatc ggcgcgattt tcgaggagcg cgacttccag ctcttttaca
     4501 aataagcctt ttccgcctac tttcgccagc ggcgtatcaa gaatcacatc gccgcgcgtc
     4561 accatcggta ccagttcaac gaccaggccc ggatggctcg ccatcaactt gtctttgaca
     4621 tagtgtgcct gccagagtgc aagtgggctt tggcgtgtgg caattcttaa aacattgtct
     4681 aacatgcttg ttaccgtcat tatcatccgt ggtccatcct aacatccttg ccagagtgat
     4741 gtcagtgttg tggtgaaacg tagacgcctg cgcaaaccgt aaaatgaggt ctggcagtgg
     4801 atcctgacag acgtttcacg ctgtcgtaat aaggaattta cagagaataa acggtgctac
     4861 acttgtatgt agcgcatctt tctttacggt caatcagcaa ggtgttaaat tgatcacgtt
     4921 ttagaccatt ttttcgtcgt gaaactaaaa aaaccaggcg cgaaaagtgg taacggttac
     4981 ctttgacata cgaaatatcc cgaatgccgc gtgttaccgt tgatgttggc ggaatcacag
     5041 tcatgacggg tagcaaatca ggcgatacgt cttgtacctc tatattgaga ctctgaaaca
     5101 gagactggat gccataaatc aattgcgtgt ggatcgcgcg cttgctgcta tggggcctgc
     5161 attccaacag gtctacagtc tactgccgac attgttgcac tatcaccatc cgctaatgcc
     5221 gggttacctt gatggtaacg ttcccaaagg catttgcctt tacacgcctg atgaaactca
     5281 acgccactac ctgaacgagc ttgaactgta tcgtggaatg tcagtacagg atccaccgaa
     5341 aggtgagctt ccaattactg gtgtatacac catgggcagc acctcgtccg tagggcaaag
     5401 ttgttcctct gacctggata tctgggtctg tcatcaatcc tggctcgata gcgaagagcg
     5461 ccagttacta caacgtaaat gtagtctgct ggaaagctgg gccgcctcgc tgggtgtgga
     5521 agtcagcttc ttcctgattg atgaaaaccg cttccgtcat aatgaaagcg gcagcctggg
     5581 gggcgaagat tgtggctcca cccagcatat actgctgctt gacgaatttt atcgtaccgc
     5641 cgtgcgtctc gccggtaagc gtattctgtg gaatatggtg ccgtgcgacg aagaagagca
     5701 ttacgacgac tatgtgatga cgctttacgc gcagggcgtg ctgacgccaa atgaatggct
     5761 ggatctcggt ggcttaagct cgctttctgc tgaagagtac tttggtgcca gcctttggca
     5821 gctctacaag agtatcgatt ccccatacaa agcggtactg aaaacactgc tgctggaagc
     5881 ctattcctgg gaatacccga acccacgtct gctggcgaaa gatatcaaac agcgtttgca
     5941 cgacggcgag attgtatcgt ttggtctcga tccatactgc atgatgctgg agcgtgttac
     6001 tgaatacctg acggcgattg aagattttac ccgtctggat ttagtacgtc gctgcttcta
     6061 tttaaaagtg tgcgaaaagc tcagccgtga acgcgcctgc gtaggctggc gtcgcgcagt
     6121 gttgagccag ttagtgagcg agtggggttg ggacgaagct cgtctggcaa tgctcgataa
     6181 ccgcgctaac tggaagattg atcaggtgcg tgaggcgcac aacgagttgc tcgacgcgat
     6241 gatgcagagc taccgtaatc tgatccgctt tgcgcgtcgc aataacctta gcgtctccgc
     6301 cagtccgcag gatatcggcg tgctgacgcg taagctgtat gccgcgtttg aagcattacc
     6361 aggtaaagtg acgctggtaa acccgcagat ttcacccgat ctctcggaac cgaatctgac
     6421 ctttatttat gtgccgccgg gccgtgctaa ccgttcaggt tggtatctgt ataaccgcgc
     6481 gccaaatatt gagtcgatca tcagccatca gccgctggaa tataaccgtt acctgaacaa
     6541 actggtggcg tgggcatggt ttaacggcct gctgacctcg cgcacccgtt tgtatattaa
     6601 aggtaacggc attgtcgatt tgcctaagtt gcaggagatg gtcgccgacg tgtcgcacca
     6661 tttcccgctg cgcttacctg caccgacacc gaaggcgctc tacagcccgt gtgagatccg
     6721 ccatctggcg attatcgtta acctggaata tgacccgaca gcggcgttcc gcaatcaggt
     6781 ggtgcatttt gatttccgta agctggatgt cttcagcttt ggcgagaatc aaaattgcct
     6841 ggtaggtagc gttgacctgc tgtaccgcaa ctcgtggaac gaagtgcgta cgctgcactt
     6901 caacggcgag caatcgatga tcgaagccct gaaaactatt ctcggcaaaa tgcatcagga
     6961 cgccgcaccg ccagatagcg tggaagtctt ctgttatagc cagcatctgc gcgggttaat
     7021 ccgtactcgc gtgcagcaac tggtttctga gtgtattgaa ctgcgccttt ccagcacccg
     7081 ccaggaaacc gggcgtttca aggcgctgcg cgtttctggt caaacctggg gcttgttctt
     7141 cgaacgcctg aatgtatcgg tacagaagct ggagaacgcc atcgagtttt atggcgctat
     7201 ttcgcataac aaactgcacg gcctgtcagt gcaggttgaa accaatcacg tcaaattacc
     7261 ggcggtggtg gacggctttg ctagtgaagg gatcatccag ttcttcttcg aagaaacgca
     7321 agacgagaat ggctttaata tctacattct cgacgaaagc aaccgggttg aggtgtatca
     7381 ccactgcgaa ggcagcaaag aggaactggt gcgtgacgtc agtcgcttct actcgtcatc
     7441 gcacgaccgc ttcacctacg gctcaagctt catcaacttc aacctgccgc agttctatca
     7501 gattgtgaag gttgatggtc gtgaacaggt gattccgttc cgcacaaaat ctatcggtaa
     7561 catgccgcct gccaatcagg atcacgatac gccgctatta cagcaatatt tttcgtgatg
     7621 aacgtgccgg aaagcgaggc ttatccggca tgcaatctta gcggaaactg actgtttcac
     7681 ccgcctgctg cgtcgccgcc tgttccagca aatcccagaa ggtttcgccg ctgcgatcgc
     7741 aaatccactc atcgcctttc agatcaaaat ggtagccgcc ttgtttggtt gccagccata
     7801 cctggtgcag cggttcctgg cggttgataa tgattttgct gccgttttca aaggtaatgg
     7861 tcagcacgcc gccgttgatt tcgcagtcga tatcgctgtc gccatcccag tcgtccaggc
     7921 gttcttcaat ggtcagccag agttgatcag ccaggcgatg aaattcactg tcgttcattg
     7981 ttgtatcctg tttttaagtg atggcggcag tatagcggca tggggtcagg gcttcaaagt
     8041 ttgcacctct gcggctgcgt tccggcacga ttcatccgtc accggaataa tgatgtctct
     8101 gtgtagcgaa agatttgtct cttcattagg gcgcagttac accacgcctt tccctgtttc
     8161 tggtaaacat tatgatcagg tttaccgagc gagcatcctc acgctgacgg aactaaaaaa
     8221 gacaacaaac aaaccacatt gcgatagtgc atagagccat cctggcgcga ggtgccgatc
     8281 acgaaactac cagcaaaaca taaatcccca cgagtaagcg ttatactcgc agcatttcct
     8341 cacttttcag acttcataaa gagtcgctaa acgcttgctt ttacgtcttc tcctgcgatg
     8401 atagaaagca gaaagcgatg aactttacag gcaatccata atgaaaaacg tgtttaaggc
     8461 actcactgta ttacttactc tcttcagcct gacgggctgc ggtctgaaag gtccgctcta
     8521 tttcccgcct gcagataaaa acgcaccgcc gccgaccaaa ccggtagaga cgcaaacgca
     8581 atccacggtg ccggataaaa acgatcgcgc cactggcgat ggtccatccc aggtgaatta
     8641 ctaaaagtca gtttctgtac ccgcgtgatt ggagtaaatg atgcagttct cgaaaatgca
     8701 tggccttggc aacgatttta tggtcgtcga cgcggtaacg cagaatgtct ttttttcacc
     8761 ggagctgatt cgtcgcctgg cggatcggca cctgggggta gggtttgacc agctgctggt
     8821 ggttgagccg ccgtatgatc ctgaactgga ttttcactat cgcattttca atgctgatgg
     8881 cagtgaagtg gcgcagtgcg gcaacggtgc gcgctgcttt gcccgttttg tgcgcctgaa
     8941 agggctgacc aataagcgtg atctccgcgt cagcaccgcc aacgggcgga tggttcttac
     9001 cgtcaccgat gatgatctgg tccgcgtaaa tatgggcgaa cccaacttcg aaccttccgc
     9061 ggtgccattt cgcgctaaca aagcggaaaa gacctatatt atgcgcgccg ccgagcagac
     9121 aatcttatgc ggcgtagtgt cgatgggaaa tccacattgc gtgattcagg tcgatgatgt
     9181 cgataccgcg gcggtagaaa cgcttggtcc tgttctggaa agccacgagc gttttccgga
     9241 gcgcgccaat atcggtttta tgcaagtggt taagcgcgag catattcgtt tacgcgttta
     9301 tgagcgtggg gcaggagaaa cccaggcctg cggcagcggc gcgtgtgcgg cggttgcagt
     9361 agggattcag caaggtttgc tggccgaaga agtacgcgtg gaactccccg gcggtcgtct
     9421 tgatatcgcc tggaaaggtc cgggtcaccc gttatatatg actggcccgg cggtacatgt
     9481 ctacgacgga tttattcatc tatgaagcaa ccaggggaag aactgcagga aacactcacg
     9541 gagcttgatg accgggcggt tgtcgattat ctgattaaaa atcctgagtt ttttatccgt
     9601 aatgcgcgcg cagtagaagc gatacgtgtg ccgcatccgg tacgcggcac cgtttcgttg
     9661 gtcgagtggc acatggcccg cgcacgtaat catattcatg ttctggaaga gaacatggcg
     9721 ctgttgatgg aacaggctat cgccaacgaa ggcctgtttt atcgcctact ctacctgcag
     9781 cgcagtctca ccgccgccag cagtctcgac gatatgctga tgcgctttca ccgctgggcg
     9841 cgcgatctcg gcctggcagg tgcgagtctg cgcctgtttc cggatcgctg gcgcttaggt
     9901 gcgccgtcga accacactca tctggcatta agccgtcagt ctttcgaacc gctgcgtatt
     9961 cagcgtttgg ggcaggaaca gcactatctt gggccgctta acggaccaga gctgctggtg
    10021 gtgctaccgg aagcgaaagc ggtgggatcg gtggcgatgt cgatgctggg aagcgatgct
    10081 gatttgggtg tcgtgctgtt taccagtcgc gatgccagtc actatcaaca agggcaagga
    10141 acgcagttac ttcatgaaat tgcgctgatg tttccggagc ttctggagcg ttggattgaa
    10201 cgcgtatgac cgatttacac accgatgtag aacgctacct acgttatctg agcgtggagc
    10261 gccagcttag cccgataacc ctgcttaact accagcgtca gcttgaggcg atcatcaatt
    10321 ttgccagcga aaacggcctg caaagctggc agcaatgtga tgtgacgatg gtgcgcaatt
    10381 ttgctgtacg cagtcgccgt aaagggctgg gagcagcaag cctcgcgtta cggctttctg
    10441 cgctacgtag cttttttgac tggctggtca gccagaacga actcaaagct aacccggcga
    10501 aaggtgtttc ggcaccgaaa gcgccgcgtc atctgccgaa aaatatcgac gtcgacgata
    10561 tgaatcggct gctggacatt gatatcaacg atcccctcgc tgtacgcgac cgtgcaatgc
    10621 tggaagtgat gtacggcgcg ggtctgcgtc tttccgagct ggtggggctg gatatcaaac
    10681 acctcgacct ggagtctggt gaagtgtggg taatggggaa aggcagcaaa gagcgccgcc
    10741 tgccgattgg tcgcaacgct gtggcgtgga ttgagcactg gcttgatttg cgcgacctgt
    10801 ttggtagcga agacgacgcg ctttttctgt cgaaactggg caagcgtatc tccgcgcgta
    10861 atgtgcagaa acgctttgcc gaatggggca taaaacaagg gctgaataat cacgttcatc
    10921 cgcataaatt acgtcactcg ttcgccacgc atatgctgga gtcgagcggc gatcttcgtg
    10981 gtgtgcagga gctgctgggt catgccaacc tctccaccac gcaaatctat actcatcttg
    11041 attttcaaca ccttgcctcg gtgtacgatg cggcgcatcc acgcgccaaa cgggggaaat
    11101 aatgcgtttt taccggcctt tggggcgcat ctcggcgctc acctttgacc tggatgatac
    11161 cctttacgat aaccgtccgg tgattttgcg caccgagcga gaggcgctta cctttgtgca
    11221 aaattatcat ccggcgctgc gcagcttcca gaatgaagat ctgcaacgcc tgcgccaggc
    11281 ggtacgggaa gcggaacccg agatttatca cgacgtgacg cgttggcgtt ttcgttcgat
    11341 tgaacaagcg atgctcgacg ccgggctgag tgccgaagaa gccagtgcag gcgcacacgc
    11401 agcaatgatt aactttgcca aatggcgcag ccgaatcgac gtcccgcagc aaactcacga
    11461 caccttaaaa cagctggcga agaaatggcc gctggtggcg atcaccaacg gtaacgccca
    11521 gccggaactg tttggtttgg gggattattt tgagtttgtg ctgcgcgctg gcccgcacgg
    11581 gcgctcaaaa ccgttcagcg atatgtactt tttggctgcg gaaaaactca acgtgccgat
    11641 cggcgagatc ttacatgttg gggacgatct caccactgac gtgggtgggg caattcgcag
    11701 cggaatgcag gcttgttgga tcagaccgga aaatggcgat ctgatgcaaa cctgggacag
    11761 ccgtttactg ccgcatctgg aaatttcccg gttggcatct ctgacctcgc tgatataatc
    11821 agcaaatctg tatatatacc cagctttttg gcggagggcg ttgcgcttct ccgcccaacc
    11881 tatttttacg cggcggtgcc aatggacgtt tcttacctgc tcgacagcct taatgacaaa
    11941 cagcgcgaag cggtggccgc gccacgcagc aaccttctgg tgctggcggg cgcgggcagt
    12001 ggtaagacgc gcgtactggt gcatcgtatc gcctggctga tgagcgtgga aaactgctcg
    12061 ccatactcga ttatggctgt gacgtttacc aacaaagcgg cggcggagat gcgtcatcgt
    12121 atcgggcaac tgatgggcac cagccagggc ggcatgtggg tcggcacctt ccacgggctg
    12181 gcgcaccgtt tgctgcgtgc gcaccatatg gacgccaatc taccgcagga tttccagatc
    12241 ctcgacagtg aagaccagct acgcctgctt aagcgtctga tcaaagccat gaacctcgac
    12301 gagaagcagt ggccgccgcg ccaggcaatg tggtacatca acagccagaa agatgaaggc
    12361 ctgcgtccgc atcatattca aagctacggt aatccggtgg agcagacctg gcagaaggtg
    12421 tatcaggcgt atcaggaagc gtgtgaccgc gcgggcctgg tggacttcgc cgagctgctg
    12481 ctgcgcgctc acgaactgtg gcttaacaag ccgcatatcc tacaacacta ccgcgaacgt
    12541 tttaccaata tcctggtgga cgaattccag gataccaaca acattcagta cgcgtggatc
    12601 cgcctgctgg cgggcgacac cggcaaagtg atgatcgtcg gtgatgacga ccagtcaatc
    12661 tacggctggc gcggggcgca ggtggagaat attcagcgtt tccttaatga tttccccggt
    12721 gccgaaacta ttcgtctgga gcaaaactac cgctctacca gcaatattct gagcgccgct
    12781 aacgccctga ttgaaaacaa taacgggcgt ctgggtaaaa aactgtggac cgatggcgcg
    12841 gacggtgagc ctatttccct ctattgcgct tttaacgaac tcgatgaagc gcgttttgtg
    12901 gttaaccgca tcaaaacctg gcaggacaac ggcggggtgc ttgccgagtg cgccattctc
    12961 taccgcagta acgcccagtc gcgtgtgctg gaagaagcac tgttgcaggc gagtatgccg
    13021 taccgtattt acggcgggat gcgcttcttc gaacgccagg aaatcaaaga tgcgctctcg
    13081 tatctgcgcc tgattgccaa ccgtaacgac gacgcggcct ttgagcgtgt ggtgaatacg
    13141 ccaacgcggg gtattggtga ccggacgctg gacgtggtac gtcagacatc gcgcgatcgc
    13201 cagttaacac tctggcaggc atgtcgtgaa ctgttgcagg aaaaagccct cgccggacgt
    13261 gctgccagcg ccttacagcg gtttatggaa ctgatcgacg ccttagcgca ggaaactgcc
    13321 gatatgccgc tgcatgtaca gactgaccgg gtaattaaag actccggcct gcgtaccatg
    13381 tacgagcagg agaagggcga aaaaggtcag acgcgtatcg aaaacttaga ggaactggtg
    13441 acggcaacgc gccagttcag ctacaacgaa gaagacgaag atttaatgcc gctgcaggcg
    13501 ttcctatccc atgcggcgct ggaagcaggc gaagggcagg cggatacctg gcaggatgcg
    13561 gtacaactga tgaccatgca ctcggcgaaa ggtctggagt tcccgcaggt gtttatcgtc
    13621 ggtatggaag agggcatgtt cccaagccag atgtcgctgg atgaaggcgg gcgtctggaa
    13681 gaagaacgcc gtctggccta cgttggcgta acccgcgcga tgcagaaact gacgctgacc
    13741 tacgcggaaa ctcgccgtct gtatggcaaa gaggtttacc atcgcccgtc gcgctttatt
    13801 ggcgagctgc cggaagagtg tgtggaagag gtgcgcctgc gcgccacggt aagccgcccg
    13861 gtcagccatc agcggatggg tacgccgatg gtcgagaacg acagcggcta caagctcggc
    13921 cagcgtgtac gccacgctaa gtttggtgaa ggcaccatcg tcaatatgga aggcagcggt
    13981 gaacatagcc gtttgcaggt ggcattccag ggccagggaa tcaaatggct ggtggcagct
    14041 tacgcccggc tggagtcggt gtaacgttgc cggatgcggt gctgcgcacc ttatttggcc
    14101 taaaaaatca ttcatattca ataaattgca acgtcatgta tgccggatag ggcgtttacg
    14161 ccgcatccgg catctgcgcc atcttcagta tctgacacaa aactatcgtt ttaacctttc
    14221 cgctcgacgg aaatcatggt gacaaaagga tagcgttgcc acgggattgc cccgcctttc
    14281 atatacatat gtgaaatcgt accatcaaga taaagcagct gctcaacgtt cagtttcgct
    14341 ttggcataac aggcaaaatc ataaaaattt gtcgcctgct ggctcaacaa aaacacggca
    14401 ttcccatgtt tattaatccc aacaccgtta cgaattttac gtgaggcgac gttgggatga
    14461 atacgcggat taattacacc gttttccatc aacattggcc ctgactgcac cgcaaactga
    14521 atatctttac tggctttaaa agcatctaga cgaacgatgc cgactttatc tcccgcgaca
    14581 taaaacacgc cgccaggacg gataaagaaa ttcccttcac ctgaagcgag atttaacgcc
    14641 accttctgct gaccgttttc gatgtacaaa ccgagcggcg catagctttc atcatagatg
    14701 ccgccgttca tcgccatctg cacctgaccc tgactattaa tatccgccag cagagcatgt
    14761 aacgttcccc acgcttcgcc attggctttt tgccagtaca ttttcacccg ctctgtttga
    14821 ggattaacgg tatacgcctg tacggtcagc gtcggatctg agagtgcgca atcatcagcg
    14881 gcaacagcaa acaagggaag taaggtgagg gtgagaaaaa tccgtttgag attcaaggtg
    14941 atcattcctt taccaatgag tagccgatgc gccattatag gtcctggatg ttggattttt
    15001 ttatcctgtt agcgaccttg acgagtacca aaaagcgcga agttcaacta ttgttctgtg
    15061 gtgttctgtt gcgtgttgac ggcaaaattt tgctggcgta acatgcgcgc acgatcactc
    15121 taagaggaca ttcgccttgg acacacccag tagatactgg ctcactatcc tgtcatccag
    15181 gatcaactcc taaggctatc cctttttgct gatagcctta gcggttgtca gcgacctcaa
    15241 tttttcccgt cgcgctgagt caggctgttt aatggtctga aacccaattt gtttctgtgt
    15301 gcccaccgaa ctgtccgata ttttaagcat tgggagtccc ggtcatgctg agcgcatttc
    15361 aactggaaaa taaccgactg acccggctgg aagtcgaaga gtcacaaccc cttgtaaatg
    15421 cagtatggat tgatcttgtc gaaccggacg acgacgagcg actgcgcgta caatctgaac
    15481 ttggccagag cctggcaacc cgcccggaac tggaagacat cgaagcatcg gcacgtttct
    15541 ttgaagacga cgacggcctg catattcact ccttcttctt ctttgaagat gcggaagatc
    15601 acgccggtaa ctccactgtg gcatttacca tccgtgatgg tcgtctgttt actctgcgtg
    15661 agcgtgaact gcccgctttt cgtctgtatc gtatgcgtgc ccgtagccag tcgatggtag
    15721 acggtaacgc ctacgagttg ctgctggatc tgttcgaaac caaaatcgaa cagttggcag
    15781 atgaaattga aaatatctat agcgacctag agcagttgag ccgggtgatt atggaagggc
    15841 atcagggcga tgagtacgac gaggcgctct ccacgctggc ggaactggaa gatatcggct
    15901 ggaaagttcg cctgtgtctg atggataccc agcgcgcgct caacttcctg gtgcgtaaag
    15961 cgcgtttacc gggtgggcaa ctggagcagg cgcgtgaaat cctgcgagat atcgaatccc
    16021 tgctgccgca taacgaatcc ctgttccaga aggtgaactt cctgatgcag gcggcaatgg
    16081 gttttatcaa catcgagcag aaccgcatca tcaaaatttt ctcggtggta tccgtggtat
    16141 tcctgccgcc gacgctcgtt gcttccagct atgggatgaa ctttgagttt atgccagaac
    16201 tgaagtggag t
//
LOCUS       DAZKHC020000091        15666 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZKHC020000091 DAZKHC020000000
VERSION     DAZKHC020000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.091"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            87..653
                     /gene="yceJ"
                     /locus_tag="ACHOSM_RS18025"
                     /old_locus_tag="pgaptmp_003605"
     CDS             87..653
                     /gene="yceJ"
                     /locus_tag="ACHOSM_RS18025"
                     /old_locus_tag="pgaptmp_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGU4560033.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLTSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            657..1232
                     /gene="yceI"
                     /locus_tag="ACHOSM_RS18030"
                     /old_locus_tag="pgaptmp_003606"
     CDS             657..1232
                     /gene="yceI"
                     /locus_tag="ACHOSM_RS18030"
                     /old_locus_tag="pgaptmp_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HGU4560034.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1274..2326)
                     /gene="trhO"
                     /locus_tag="ACHOSM_RS18035"
                     /old_locus_tag="pgaptmp_003607"
     CDS             complement(1274..2326)
                     /gene="trhO"
                     /locus_tag="ACHOSM_RS18035"
                     /old_locus_tag="pgaptmp_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGU4560035.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2551..3471
                     /gene="lpxL"
                     /locus_tag="ACHOSM_RS18040"
                     /old_locus_tag="pgaptmp_003608"
     CDS             2551..3471
                     /gene="lpxL"
                     /locus_tag="ACHOSM_RS18040"
                     /old_locus_tag="pgaptmp_003608"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HGU4560036.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3643..4869
                     /gene="mdtG"
                     /locus_tag="ACHOSM_RS18045"
                     /old_locus_tag="pgaptmp_003609"
     CDS             3643..4869
                     /gene="mdtG"
                     /locus_tag="ACHOSM_RS18045"
                     /old_locus_tag="pgaptmp_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HGU4560037.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4952..5326
                     /gene="msyB"
                     /locus_tag="ACHOSM_RS18050"
                     /old_locus_tag="pgaptmp_003610"
     CDS             4952..5326
                     /gene="msyB"
                     /locus_tag="ACHOSM_RS18050"
                     /old_locus_tag="pgaptmp_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU4560038.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5327..5554)
                     /gene="yceK"
                     /locus_tag="ACHOSM_RS18055"
                     /old_locus_tag="pgaptmp_003611"
     CDS             complement(5327..5554)
                     /gene="yceK"
                     /locus_tag="ACHOSM_RS18055"
                     /old_locus_tag="pgaptmp_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU4560039.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5727..8270)
                     /gene="mdoH"
                     /locus_tag="ACHOSM_RS18060"
                     /old_locus_tag="pgaptmp_003612"
     CDS             complement(5727..8270)
                     /gene="mdoH"
                     /locus_tag="ACHOSM_RS18060"
                     /old_locus_tag="pgaptmp_003612"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HGU4560040.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8263..9798)
                     /gene="mdoG"
                     /locus_tag="ACHOSM_RS18065"
                     /old_locus_tag="pgaptmp_003613"
     CDS             complement(8263..9798)
                     /gene="mdoG"
                     /locus_tag="ACHOSM_RS18065"
                     /old_locus_tag="pgaptmp_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HGU4560041.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYETPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10192..11349
                     /gene="mdoC"
                     /locus_tag="ACHOSM_RS18070"
                     /old_locus_tag="pgaptmp_003614"
     CDS             10192..11349
                     /gene="mdoC"
                     /locus_tag="ACHOSM_RS18070"
                     /old_locus_tag="pgaptmp_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HGU4560042.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11357..12838)
                     /gene="clsC"
                     /locus_tag="ACHOSM_RS18075"
                     /old_locus_tag="pgaptmp_003615"
     CDS             complement(11357..12838)
                     /gene="clsC"
                     /locus_tag="ACHOSM_RS18075"
                     /old_locus_tag="pgaptmp_003615"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HGU4560043.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12780..13313)
                     /gene="ymdB"
                     /locus_tag="ACHOSM_RS18080"
                     /old_locus_tag="pgaptmp_003616"
     CDS             complement(12780..13313)
                     /gene="ymdB"
                     /locus_tag="ACHOSM_RS18080"
                     /old_locus_tag="pgaptmp_003616"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HGU4560044.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13408..13719)
                     /gene="ymdA"
                     /locus_tag="ACHOSM_RS18085"
                     /old_locus_tag="pgaptmp_003617"
     CDS             complement(13408..13719)
                     /gene="ymdA"
                     /locus_tag="ACHOSM_RS18085"
                     /old_locus_tag="pgaptmp_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HGU4560045.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQVASVKVQYIDKQKKLAVMNIEY
                     N"
     gene            complement(13840..14172)
                     /gene="csgC"
                     /locus_tag="ACHOSM_RS18090"
                     /old_locus_tag="pgaptmp_003618"
     CDS             complement(13840..14172)
                     /gene="csgC"
                     /locus_tag="ACHOSM_RS18090"
                     /old_locus_tag="pgaptmp_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HGU4560046.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
     gene            complement(14230..14685)
                     /gene="csgA"
                     /locus_tag="ACHOSM_RS18095"
                     /old_locus_tag="pgaptmp_003619"
     CDS             complement(14230..14685)
                     /gene="csgA"
                     /locus_tag="ACHOSM_RS18095"
                     /old_locus_tag="pgaptmp_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HGU4560047.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14726..15181)
                     /gene="csgB"
                     /locus_tag="ACHOSM_RS18100"
                     /old_locus_tag="pgaptmp_003620"
     CDS             complement(14726..15181)
                     /gene="csgB"
                     /locus_tag="ACHOSM_RS18100"
                     /old_locus_tag="pgaptmp_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HGU4560048.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3909 a         3992 c         3712 g         4053 t
ORIGIN      
        1 tatcattcaa tttgttattt ttgtgttgca attcattctc cgacacgcca ttttctttct
       61 ctcgcaaatt cgtattggat gcacttatgt cattcacaaa tacccctgaa cgctatggtg
      121 ttatatctgc ggccttccat tggctaagcg caattattgt ctacggcatg tttgctttag
      181 gtctgtggat ggtcacgctc agttattacg atggctggta tcacaaagca cccgaactgc
      241 ataaaagcat cggtattttg ttaatgatgg ggctggttat tcgcgttctg tggcgtgtca
      301 tatctccacc gccgggtccg ctgacgagtt attcgcccat gactcgtctt gccgcaaagg
      361 ctggacatct cgcattgtac ctattgctgt tcgccatcgg catcagcggc tatctgatct
      421 caactgccga tggtaaaccg atcagcgttt ttggctggtt tgacgtcccc gcgacccttg
      481 ccgacgctgg cgcacaggca gactttgccg gtgccctgca tttttggctc gcgtggagcg
      541 tcgtagtact gtccgttatg cacggattta tggccctgaa gcatcatttc atcgataaag
      601 acgacactct gaagcgcatg ctgggaaagt cgtcatctga ctatggagta taaaaaatga
      661 aaaaaagcct gcttggttta accttcgcgt ccctgatgtt ctctgccggt tcagcggttg
      721 ccgccgatta caaaattgac aaagaaggtc agcacgcctt tgttaatttc cgcatccagc
      781 accttggcta tagctggtta tacggcacct ttaaagattt cgacggtact tttacctttg
      841 acgaaaaaaa tccggctgcc gataaagtga atgtgacaat taacaccacc agcgtcgata
      901 ctaatcacgc cgaacgcgat aaacatcttc gcagtgcaga tttcctcaat accgcaaaat
      961 atccacaggc aacattcacc tccaccagcg tgaagaaaga cggtgacgaa ctggatatta
     1021 ccggcgatct gacgctgaat ggcgtaacca aacccgtcac gctggaagcg aaattaattg
     1081 gtcagggcga cgacccatgg ggtggtaaac gtgcaggctt cgaggccgaa ggcaaaatta
     1141 agctcaagga cttcaatatc aagacagatt taggtccagc ttctcaggaa gtggatctga
     1201 ttatttcagt ggaaggcgta cagcagaagt aagcgtgaaa tgttgccgga tggcacgcat
     1261 ccggcaatga tatttattct gttggatcag gaatgcccag tgttgtattc agacgtccac
     1321 gagacttatt aaagatctta ttgccatttt cacgtcctgc ccgacggcgt cgctgttctt
     1381 ctggcggtaa cgcgctttct tcgcagcaaa tctcactaca acaacctttg tatttttccg
     1441 cgcatactgg acactgaata aacagcaggt ggcagccatc atttttacag ttggtatggc
     1501 tgtcgcacgg cgcgccgcac tggtggcaat gcgcgataat ctcatccgat atacgttcgc
     1561 ccatccgctc gtcaaaaaca aaatttttgc caataaaacg caccggcaag ccctgctcgc
     1621 gcgccttacg ggcgtattca ataattccgc cctcgatatg ccagactttg ttgaatccgt
     1681 tatgtttcat ccaggcactg gccttttcac aacgaatgcc gccggtgcag tacatgacga
     1741 tttttttatc tttatgtgcc tgcatcatct cgactgcttt tggcagctgc tcacggaagg
     1801 tatctgccgg aatttcgagc gcgttttcaa agtgccccac ttcatactca tagtggttac
     1861 gcatgtcgat aaatagtgca tcgggatcgt caagcatggc gttcacttcc gccgcttgca
     1921 gatactcacc aacattgctg gcatcaaagt gaggatcgtc aataccgtcg gcaacaatgc
     1981 gatcgcgtac cttcatgcgc agtacccaga aggatttccc gtcatcatcc aacgcgatat
     2041 tcaggcgtaa accctctaaa gccgggtcga aggcatagag ctgcgcgcga aatgtttcaa
     2101 cattgctcgc aggtacgctg atttgcgcgt taatgccctc atgcgccaga tacactcgcc
     2161 caaaaacatt cagcgcggta aacagctgat ataaagcgtc acgggtcgcc ttaggatcgg
     2221 cgatgtggaa atacttgtaa aacgaaatgg tggttcgcgg ttcgctctca gccaacattt
     2281 tggcttttag cgcgtcgttg gaaatgcggt tgtgtaacac tggcatggtg tacggttcct
     2341 gcgagatggg aaagtaaaaa tccgcggcat gatatagcaa ttatcgataa ttaacatcca
     2401 cacattttac gctacatttg cgcattaaaa attatttgtt atttacaagc gcggcaattt
     2461 cgcccagtct tcagccacaa attttggttg cgggcgaaaa aatgcgacaa tacatacaat
     2521 tgcccgaata ggttgaaaaa caggattgat atgacgaatc tacccaagtt ctccaccgca
     2581 ctgcttcatc cgcgttattg gttaacctgg ttgggtattg gcgtactttg gttagtcgtg
     2641 caattgccct acccggttat ctaccgcctc ggttgtggat taggaaaact ggcgttacgt
     2701 tttatgaaac gacgcgcaaa aattgtgcat cgcaacctgg aactgtgctt cccggaaatg
     2761 agcgaacaag aacgccgtaa aatggtggtg aagaatttcg aatccgttgg catgggcctg
     2821 atggaaaccg gcatggcgtg gttctggccg gaccgccgaa tcgcccgctg gacggaagtg
     2881 atcggcatgg aacacattcg tgacgtgcag gcgcaaaaac gcggcatcct gttagttggc
     2941 atccattttc tgacactgga gctgggtgcg cggcagtttg gtatgcagga accggggatt
     3001 ggcgtttacc gcccgaacga taatccactg attgactggc tacaaacctg gggccgtttg
     3061 cgctcaaata aatcgatgct cgaccgcaaa gatttaaaag gcatgattaa agccctgaaa
     3121 aaaggcgaag tggtctggta cgcaccggat catgattacg gcccgcgctc aagcgttttc
     3181 gtcccgttat ttgccgttga gcaggctgcg accacgaccg gaacctggat gctggcacgg
     3241 atgtccggcg catgtctggt gcccttcgtt ccacgccgta agccagatgg caaagggtat
     3301 caattgatta tgctgccgcc agagtgttct ccgccactgg atgatgccga aactaccgcc
     3361 gcgtggatga acaaagtggt cgaaaaatgc atcatgatgg caccagagca gtatatgtgg
     3421 ttacaccgtc gctttaaaac acgcccggaa ggcgttcctt cacgctatta aatctcccct
     3481 gccggatgct tcagaatggc atccggcatt accacagcaa atccccctga tttagcgata
     3541 aaagctctct ggattgcgcc ccctggaagt cgggcgcata attagtgtgc ttatcttttc
     3601 ttcttatgtt caccgcgcct ggcgcaccaa cagcggattg ctatgtcacc ctgtgaaaat
     3661 gacaccccta taaactggaa acgaaacctg atcgtcgcct ggctaggctg ttttcttacc
     3721 ggtgccgcct tcagtctggt aatgcccttc ttacccctct acgttgagca gcttggcgtt
     3781 accggtcact ccgccctgaa tatgtggtcc ggtattgtct tcagcattac atttttattt
     3841 tcggccatcg cctcaccgtt ttggggtgga ctcgccgacc gtaaaggccg aaaactcatg
     3901 ctattacgct ctgccctcgg catgggcatc gtgatggtgt tgatggggct ggcacaaaat
     3961 atctggcagt ttttgatcct gcgggcgctt cttgggttac ttggcggatt tgtccccaac
     4021 gctaatgctc ttatcgccac acaagtaccg cgtaataaaa gcggctgggc gctgggtacg
     4081 ctctccacag gcggcgttag tggtgcgttg ctcggcccaa tggctggcgg cctgctcgcc
     4141 gatagctacg gcttacgtcc ggtattcttt attaccgcca gtgtgctcat actctgcttt
     4201 ttcgtcaccc tgttttgcat cagagaaaaa ttccagccgg tcagcaaaaa agagatgctg
     4261 cacatgcggg aagtggtgac atcacttaaa aacccgaaac tggtactcag cctgtttgtc
     4321 actacgttaa tcatccaggt ggcgacgggc tcaattgccc ccattctgac gctgtatgtc
     4381 cgcgaactgg cgggtaacgt cagtaacgtc gcctttatca gtggcatgat cgcctcggtg
     4441 ccaggcgtgg cggctctgct aagtgcacca cgactcggca aacttggcga tcgaatcgga
     4501 cccgaaaaga tcctgattac agcgctgatc ttttctgtac tgctgttgat cccaatgtct
     4561 tacgttcaga cgccattgca acttgggatt ttacgttttt tgctcggtgc cgccgatggt
     4621 gcactactcc ccgccgtaca gacattgttg gtttacaact cgagcaacca gatcgccggg
     4681 cgtatcttca gctataacca atcgtttcgt gatattggca acgttaccgg accattgatg
     4741 ggagcagcga tttcagcgaa ctacggtttc agagcggtat ttctcgtcac cgctggcgta
     4801 gtgttattca acgcagtcta ttcatggaac agtctacgtc gtcgtcgaat accccaggta
     4861 tcgaactgat ttttcgcctt tcatacttgc aaaagcggag aatcagctat ccttttccct
     4921 gaaacctcat caactcaaag ggagaatcgt gatgaccatg tacgcaacgc ttgaagaagc
     4981 cattgacgct gcacgcgaag aatttcttgc agacaacccc ggcatcgacg ccgaagatgc
     5041 gaatgtgcaa cagttcaatg cccaaaaata cgttttgcag gacggcgaca tcatgtggca
     5101 agttgagttt tttgccgacg aaggggaaga aggtgaatgt ttacctatgc ttagcggtga
     5161 agccgcgcaa agtgtttttg atggcgacta tgatgagata gagatacgcc aggagtggca
     5221 ggaagagaat acattacatg aatgggacga gggggaattt cagcttgagc caccgctgga
     5281 taccgaggaa ggacgcgcag cagctgatga gtgggatgaa cgttaatcac tcatacgggc
     5341 catgatgaat gtcgatcggc agcagtaaag tatcaaaaac cagggagaat ggcagatcaa
     5401 ggattgtgac ataacgccag gcggagtcac gcacatccca ttgcacaccg ggataatatt
     5461 ggttgccatg cccctgcccc ggtatagtgc gactaataat gctgccacag ccactcagca
     5521 aggtcaccat gatgctcacc acaattaaac gcatttgata cctcttccgt aaaaaaatac
     5581 cggcataacg ccggtatttt tattggctcg ttgtttttgc cggatgcggc gtgaacgcct
     5641 tacccaacct acaaaatctt gcaaaatcaa taaattgcag gaacgatgta ggcctgataa
     5701 gcgtagcgca tcaggcaact acgtttttat tgcgaagccg catccggttt acgcaatgcc
     5761 agtggattga gctttatccc ttcgtaataa ctcacccatg aagaatatct ctccggggaa
     5821 ttccagacac ggaaatgcag acgggccatc gtcaccggat cgcttagcag caccaggcga
     5881 cgatcgcgat tcagcttctc tggcgtctcg ttaagcgcct gttcaacgtg gcggtcacgg
     5941 gcgatttcca gcaccttact ggcgcggtga cgcgcggtcg ccattgcggt tgccagagcg
     6001 ttaaatgacg ggttaaacac cgcgtgcata aaaccatcat caagggagcg ttgacgattc
     6061 atctcaagga agcgatcggt atcaaccagc acctgcggcg gtgaatactc ttccgggatc
     6121 aggaacagtt tccagcgttt ggttcgcaga ccaacggtgg cacggctgga aatcaccgaa
     6181 acaaacggtg acaggatcaa cgagaagaca atcggtgcca gccagaacag gaaacgcaga
     6241 tccagccacg ccatcccaac agcccacact aaccctaaca gcagctgtga gccgtggcgt
     6301 ttgaacgctt caccccagga agtggagtca tcatcacgct gcggtgaatt ccacaccact
     6361 tcccagccaa ggaacgcgct gacaacgaag accgtatgga acagcatgcg taccggagcc
     6421 agcagcacgg aaaaaagcac ttccagtagc aacgataatg taacgcgcca gaagccgccg
     6481 tattctttcg ttcctttgca ccagataagc aaaatgctca ataacttcgg caggaacaac
     6541 agcaccatag tcgaagcaaa aagtgcaatc gccagctcag gacgccactg cggccacacc
     6601 gggaacaact gccgtggttg caggaagtat tgcggttcgg tcaacgcatg tactacctgc
     6661 aatgcagtag agagcgcgag gaacataaac cacagcggag cggagagata agacatcacg
     6721 cccgtcagga acaccgcacg gtgaaccggg tgcataccct tcaccaggaa cagacggaag
     6781 ttcatcaggt taccgtggca ccagcggcgg tcacgtttta gctcatcaag caagttaggc
     6841 ggtaattctt cataagaacc cgggagatcg taagcaatcc agacccccca acctgcacgg
     6901 cgcatcaacg ccgcttctac aaagtcatgt gacaggattg aaccggcaaa ggaaccttcg
     6961 cccggcagcg gagccagtgc gcagtgctcg ataaacggtt tcacgcggat aatcgcgtta
     7021 tgtccccagt agtgcgactc gccaagttgc cagaagtgca aaccggctgt aaacagtggc
     7081 ccatacacgc gggtcgcgaa ctgctgacag cgcgcataca gcgtatccat accggacgct
     7141 ttcggcgacg actgaatgat cccggcgttc gggttggctt ccatcaggcg caccagcccg
     7201 cacaaacaat caccggtcat taccgagtca gcatccagca ccaccatgta gctgtactgg
     7261 ctgccccagc gacggcagaa gtcatcgata ttaccgcttt tacgcttcac gcggcgacgg
     7321 cggcggcgat agaaaatctg accttcgcca ccgacttcag cgataagctc catccaggct
     7381 ttttgctctg cgacgcagat atccgggtta taactgtcac taagaatgta gacatcaaag
     7441 tgtttggcat tcccggtggc ttttactgat tcccacgttg cacgcaggcc agcaaaaaca
     7501 cggttcacgt cttcgttaca gataggcatg atcaacgccg tgcgatgctc cgggtttaat
     7561 ggttcatcgc caactgttga cgcagatata ctgtatttat cgcgaccaat aagcagttgc
     7621 aggaagccca ttaacgccgt ccagaatccg gcggacaccc aacagaacag taccgcaaag
     7681 aggatcagga taccggtttg cagcatataa ggcagaagct gcataaagga aacccacaaa
     7741 tcctgaccaa ccatatccat aggattaatc agcgcccaac cctgataagg aagaatggtc
     7801 ttcatatacc aggtcgcgac gacagtttgc gcgagcgtca ggatcaacag aatgtaacgg
     7861 cggatggtac cgacggtacg ccacttttgc tcgctctcct gctcttcttt ggtcaaacga
     7921 gccagatagc gcggcgtgac atcgcgtcca cgcaggcgat cccagaaacg gcctaccggg
     7981 ttggtacgcc acgggtcggg aaacatcgag gagcgttttg cttctggcat cgccttcagc
     8041 tgatcgcgcc cttcgtcgtc tttaattaac tgtccatcag caagtgaatc tggccaggct
     8101 tgttccagac gcgcctttac cgagccttgc ggggaatcat cctcccgcgc ccaggtgcgg
     8161 tgttcggcat ccagcgcctg atgaacggcg cggatatcag tcttcggcaa tgccgctttc
     8221 tcgcttgcgg cgatgggcat tgcgtcaatg tactcagttg tcttattcat tggcaggtaa
     8281 ctggtagctc caggtttcac tcaacgtctg atcggcattc accagcgcag cacgcatttc
     8341 agtggttttc ttggcatctt tcactttcac acgcatcacc agacgccagc ctttggtaac
     8401 cgggttatag cgcaccgtgc tttcaactat ctcaccatta tcaccaatgc tggtttgcgc
     8461 tgtgaccggg gtatcctctg gcagtttttt catctctgcg ccggtaaaat cgaccacaaa
     8521 ggcgatagta ccgtcaggct ggcgaatcag gttcgactgc ttcacatccc ccgttgaacg
     8581 acgcgtttgt tgcacccatg cgttatctgg cgcatgcagt ttgtcttcat cacggctgaa
     8641 ggtgatggtg tatttaaagt tcatctcttt acccggctcc ggcagctgat ccggcgtcca
     8701 gtaagcgacg atgttatcgt tggtttcatc gttggttgga atttccacca gctcaacgct
     8761 gcctttaccc cactcccctt tcggagtcac ccatgcgctt ggacggagat cgtaacgatc
     8821 atcgagatct tcaaagcggg agaaatcacg accgcgctgc aacagaccaa agccttgcgg
     8881 gttttccatg gagaagctgc tgaccgctaa atgtttcggg ttattcaacg gacgccagat
     8941 ccattcgcca ttaccggcat ggatagagag accgttagag tcgtgcaact ccggacgata
     9001 gttatttgca ggcgacggtt ggttcggccc aaacaggaac atactggtta acggtgcaac
     9061 ccccagtttg ccgactttat cgcgcagata gattttcgac tgcacatcca caaccgtgtc
     9121 acgccctggc ataaccacga atttgtaagc acctgtcgcg cgcggcgagt caagcaatgc
     9181 atagatggtt aaacgtttat cagtcggttt tggacgctcg atccagaact ctttgaagcg
     9241 cggaaattct tcacccgatg gcaaggcggt atcaattgcc aggccgcggg cagaaaggcc
     9301 ataaacctga cctgcgccaa tcacgcggaa atagctggcc ccgagcatgc tgacgatttc
     9361 atcgttttta tctttgctgt tgatcgggta aagcacttta aaaccggcaa agccaaggtc
     9421 ttttaccgta tctttgtcat gctgaacatc gccgaaagtg aaataatccg ggctgtattt
     9481 gattcgtttg actgcggtgg cagtcacttc atttattttg accggggtat cgaagtacat
     9541 accctgatgg tagaactcga gtttgaatgg ggtcttcaga ttgttccagt acgctttgtc
     9601 atgattaaac tggatctgct gatagtccgc gtatttcata tcgcggaaaa cggagggcaa
     9661 gttgcttttg ggcgtctcgt agcctttccc ggctaaagat tgagcttgct ttgcgacatc
     9721 atcaatactg aaagcccagc ttgaagatgt atacagggtt aacattactg cagcactcaa
     9781 ccaacgcatt ttcatcattt gtagtttatg tttcataatt agtaagcact tccccctttg
     9841 tgtgcttata tcgatccgat ctattttaat ggataaccag ccattccgac aacagaatcc
     9901 cggtatcgtt cagcacgctg gctcatggat taagcacctg attgcaaccg ttttcacttt
     9961 ccgtgcttct cctaaagaca gaagcatcga cttcgtgtag ggtttgccag caaatttatt
    10021 tatcaaccgt cttacagacg cttccattct ggttagtggt agcgatgatg ctatgcataa
    10081 aaaccttgaa gcctgcgcct ggcgcggtca ggtcggtgtc actatggcaa caagcctaaa
    10141 ggcataaccc gacagggata agacgaaaaa tccgagatta gttaaccata tatgaaccca
    10201 gtacccgcgc aacgtgaata tttcctcgac tccatccgcg cctggctgat gttgttaggg
    10261 ataccttttc atatttcttt aatctattcg agccatacat ggcatgtgaa tagcgccgaa
    10321 ccatcattgt ggctgaccct ttttaatgac ttcatccact cgttccgcat gcaggtattt
    10381 ttcgttatat ccggctactt ttcctacatg ctttttttac gctatccctt gaaaaaatgg
    10441 tggaaagttc gtgtcgaacg tgtaggtatc ccgatgttaa cagccatccc cctactgaca
    10501 ttaccgcaat ttattatgct gcaatatgtc aaaggaaaag cggaaagttg gcctgggctg
    10561 tcattgtatg acaaatataa tacgttggcc tgggaattaa tatcacacct gtggttttta
    10621 ctggtgttag tagtcatgac gacgctgtgc gtatggatat ttaagcgcat cagaaataat
    10681 ttagaaaatt ctgataaaac gaataaaaaa ttctcgatgg taaaactatc ggtgattttt
    10741 ttatgcctcg gcatcggtta tgcggtaata agaagaacga tttttattgt gtatccgccc
    10801 attctgagta atggcatgtt caattttatt gtcatgcaaa cgctgtttta tttgccgttc
    10861 tttatcctcg gcgcactggc tttcattttc cctcatctta aagccttgtt taccacgccg
    10921 tctcgtggct gtacccttgc agcagcattg gcgtttgtcg cttatttact caaccagcgc
    10981 tatggcagtg gcgatgcctg gatgtacgaa accgagtcgg tgatcaccat ggtcctcggt
    11041 ctgtggatgg tgaatgtggt cttctccttt ggccaccgtt tgcttaactt ccagtcagcg
    11101 cgggtgactt attttgttaa cgcatcgctg tttatctatc tggttcacca cccgttaacg
    11161 ctgtttttcg gcgcatacat tacaccgcac atcacctcca actggcttgg ttttctctgt
    11221 ggcctgatat ttgtagtagg gattgcgata attctgtatg aaattcattt gcgcatcccg
    11281 ttactgaagt ttttgttctc tggtaaaccg gttgttaagc gtgagaacga taaagcacca
    11341 gcccgttaag ccacatttac aataaccatt ccacgggcaa tatcgacgcc agtctgacca
    11401 taacccgctt ccagaaactg gtggcgggtt cttttttgag aataatctct ttctttgcat
    11461 gacgatcaac ccagttgatc cgtccccacc tgtccagacg gagctgccag gccgcatcat
    11521 actggctctg aataaagcgt ttatcaatta actgtgccag cgtttcgctc tctatcacga
    11581 agcccatttc agtattgagc aatgttgaac gcggatcgaa attgaaagag ccgataaaca
    11641 ccgttttacc atcgatgcta aaggttttag catgcaggct ggctccggaa ttaccggtta
    11701 tgccgcgatc gtgtaacgta ctactttgtt cacgcgtcgg cttgagttca tataattcca
    11761 cgccatagcg gagcaatttt ttgcgccagc gcgcgtatcc ggcatggacg acagcaacat
    11821 cgttagcggc aagagaattg gttaggatcg caatctttac cccttttctc accatccgta
    11881 agagttgcgc cacacctgcg cgtgtcggta caaaatagga agagataata tcgatgcgtt
    11941 cactgggtga gcccatgata tcgaacaggc gctgcggtaa cagtgaatgc cgttttgcct
    12001 tgccctcccc tttcgccgga tcatcactta ataaacgtgt cttcgcccag ataagcggca
    12061 atgttccatc aaccagatga tttataaatg gactggattc cattttgcgt aaataacgat
    12121 gcgtcatggc atcgttatgc caggaggcgg gtaactcgat gcgatccgcc atttcaccct
    12181 ccgggacatc cagcacctgc tgtaagggtg aaaccgattt gcaataccag tagcgggcga
    12241 aatcatcggc aacgtcctct accacgggtc ctattgccat gacatctaaa tccgaaaaaa
    12301 gtggctcctc ccctgctcca aaataggcat caccaatatt tcgtcctccc accagggtca
    12361 ccacgccatc gacagtgaaa cttttattgt gcatacggcg attaagacgg gaaaagtcgg
    12421 tgatataacc aagcggacgc agcaagcgaa acgagaaagg attaaaaagc cggacttcaa
    12481 tgcgtggatg actgtcaagc aagcgtaaaa tgtcgtcaag tccgggcgta ttgttgtcgt
    12541 ccagcaacaa acggacgcga acgccacgct ttgctgcggc taacagggcg gaaaacagta
    12601 accgacccga catatcgtcc tgccagatgt aatactgaac atcgagcgta tgctctgcca
    12661 tttcggccag acgataccgg gcggcaaacg catccagact tttctccaga ggaaaaaggc
    12721 cacactgacc gggatgttgc gaacacagtg gcagcaccgc gctcgccagc cggggcaaat
    12781 cattcatctc cttgttgggt aaggagtctt tcgtagaggt gggcgttttc ttcatcataa
    12841 cagacaaagt atacctgttc gggtaaagcg tgacgggtaa taaattctga aacggttttt
    12901 actgcgattt cagccgctgc cgcacgaggg taaccataaa ccccagtact gattgcagga
    12961 aaagccactg acgtatagct gtttgccgcc accagtcgta ggctattgag ataggcatcc
    13021 tgcaaaagct ggtcttcgtt ttgttcacca ccacgccaga ctggcccgac ggtgtgcact
    13081 acggctttag cgggaagatc gcctgcaagc gtaataacgg catgccccgt agggcaatcg
    13141 ccctgctgtt gcctgacttt taaacaggca tccagtaggg ccggacccgc tgcgcgatga
    13201 atggccccat cgacgccgcc acctcccatt aatgaaggat tagccgcatt cacaatcaca
    13261 tcaacggcca gtttggtaat atcaccctgc acaacatgaa tacgcgtttt cataacgcct
    13321 ccttacacct tcttgtttct taagtgtaca gcaggctttt tggttaaatc gcatttttct
    13381 actcagcaac gtgagttgtt cagaaactta gttatattcg atgttcatta cagccagctt
    13441 tttctgttta tcaatatact gcacttttac tgaggctact tgcgcgtttt tgacattgcc
    13501 gctcattaac gctttgctgg aataggtttt tcccggaaca gaaccattct gtgggcagtt
    13561 catttctacg ggtgatgact gggtgctgac gtcacatgcc ggttccacaa tttggccata
    13621 aaaatggatc acgccccctt gcgcggtgct tcctgcgatg gcaataaaag gaaaaaacaa
    13681 actgccgagc ataagagagt ttaaaaaagg tctgaacatt aattctattt cctcaatgat
    13741 tagtcatcct tgagggttgt gttatccata ctttcaggtt ggctgattcg cagcagacca
    13801 ttctctccag attcatctta tgctcgatat ttcaacaaat taagactttt ctgaagaggg
    13861 cggccattgt tgtgataaat gaagtgcctg tccatcagaa acagtaacaa ctattttcac
    13921 ccgatcttcc ggggaaatat ttaaactcaa cttcgtcaaa gcaataggtt gattagcagg
    13981 caatgagagg gtcttttctt gcttcgtctg actttgccct gaactgcctt cgcgcaggga
    14041 caatatttgt actctgcaca gacaagattg agtaagagtg acttcaggaa taatggtata
    14101 cacatcccct tgctgggtcg tattaaaggt tatctggctg gaaagtgccg caaggagtaa
    14161 taacgcattc atattcttct cccgaaaaaa aacagggctt gcgccctgtt tctttaatac
    14221 agatgtgtat tagtactgat gagcggtcgc gttgttacca aagccaacct gagtcacgtt
    14281 gacggaggag ttagatgcag tctggtcaac tgcagcaccg ttgccaccac cgaactgttt
    14341 aacagtcatt tcagaatttt tgccgttcca ctggtcaaga gtggcgctgt taccgaagcc
    14401 acgttgggtc agatcgattg agctgtcatc tgagccctga ccaacatctg caccattacc
    14461 accgccatgc tgggtaatag tcaagtcaga gttacgggca tcagtttgca gagcaagtgc
    14521 agagttaccg ccaccgtact ggtaaatgtt cagctctgaa tttgggccgc tattattacc
    14581 gccaccaccg tggttaccgc cgccgccgta ctgaggaaca acacctgcca gagcgctacc
    14641 ggagaatacg attgctgcaa ttgctgctac ttttaaaagt ttcatgtaaa acccccatcg
    14701 gattgattta aaagtcgaat ggaaattaac gttgtgtcac gcgaatagcc atttgcgact
    14761 gtctctgcac tacaattgcc gttttttgag taccatactg tgtaatattt gctttattac
    14821 cagaaccttt ctggataatc atcgcagtat taccataagc accttgcgaa atactggcat
    14881 cgttggcact gcccgcctga tcaatatatg caaggttata atctcctgtc tggtcaatct
    14941 ttgcccggtt gctactacct tcttgcgcaa caaccgccaa aagttttgag cctccctgcc
    15001 gtaactgagc actattatta gtcccagctt gaccaattat ggctgcctga ttaaatgaag
    15061 acttactcaa ttcatttacc gcgaagttat attctgaatt agctaaatca taacctgctg
    15121 cggctgcaat cccaggcgca cccagtattg ttaacatcat aaataacaat ttgtttttca
    15181 tgttgtcacc ctggacctgg tcgtacattt aagaaattaa atcatttcaa cttggttgtt
    15241 aacgcaacct gtattttgtt aacgctgcgt tacgatggaa agtatgtctg cggaaatatt
    15301 tttaataact cacccgcgcg ttgtattttt ctttttttgt tcataccaaa gtattaaaac
    15361 tcacgtaaat acgctgttat ctacgcaaaa aatattttgt tttcttttaa atctccgttt
    15421 tccgctatca aaaatcacca gacaatcatt cttcttgccc gtcgctgatt gctgcgatat
    15481 gtcttgcgca caagccgtga caaacctcgt ttttttgcag taaaaaattg tccacggtgg
    15541 tgcggagaaa aaacaagaac gtttaacatg acgaaaggac tacaccgaaa tattttttat
    15601 atgcattatt agtaagttag caccatttgt atgatttttt aaaattgtgc aataaaaacc
    15661 aaatgt
//
LOCUS       DAZKHC020000092        15278 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZKHC020000092 DAZKHC020000000
VERSION     DAZKHC020000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.092"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..933
                     /locus_tag="ACHOSM_RS18105"
                     /old_locus_tag="pgaptmp_003621"
     CDS             <1..933
                     /locus_tag="ACHOSM_RS18105"
                     /old_locus_tag="pgaptmp_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4560049.1"
                     /translation="EAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKNGDIA
                     ATTHKEKARMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLWVDIY
                     KEAQHAGEVPPGWNPPEATRKPIPKVTRARLTLEDWQKIYNATPEKHFIRNAMLLAIV
                     TGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDRI
                     LSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGITPPAGKTPPTFHEQRSLSERLYR
                     AQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVV"
     gene            complement(1010..1477)
                     /locus_tag="ACHOSM_RS18110"
                     /old_locus_tag="pgaptmp_003622"
     CDS             complement(1010..1477)
                     /locus_tag="ACHOSM_RS18110"
                     /old_locus_tag="pgaptmp_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HGU4560050.1"
                     /translation="MPDQKESENTKLTCEEQKDNELVSRVIENPEVLNRVLDSPQVRA
                     IVCQHFQGPVPPPSMLKKYDQLVPGLANRLVELTEKEQAHRHKTVADSIDIARDGQTK
                     AFWLAILIILAATVFGVMGETVLAGTLVSIDLVALVTAFIVGKHYSKQEPDQD"
     gene            complement(1455..1658)
                     /locus_tag="ACHOSM_RS18115"
                     /old_locus_tag="pgaptmp_003623"
     CDS             complement(1455..1658)
                     /locus_tag="ACHOSM_RS18115"
                     /old_locus_tag="pgaptmp_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560051.1"
                     /translation="MKRKSVALLQAIGSVIALSPTVVSANGMPANPVSALRQDAAAIR
                     GDFQKAMVSVNVEKLNNARSKRK"
     gene            1967..2761
                     /gene="potC"
                     /locus_tag="ACHOSM_RS18120"
                     /old_locus_tag="pgaptmp_003624"
     CDS             1967..2761
                     /gene="potC"
                     /locus_tag="ACHOSM_RS18120"
                     /old_locus_tag="pgaptmp_003624"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HGU4560052.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2758..3804
                     /gene="potD"
                     /locus_tag="ACHOSM_RS18125"
                     /old_locus_tag="pgaptmp_003625"
     CDS             2758..3804
                     /gene="potD"
                     /locus_tag="ACHOSM_RS18125"
                     /old_locus_tag="pgaptmp_003625"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HGU4560053.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3960..4781)
                     /gene="cobB"
                     /locus_tag="ACHOSM_RS18130"
                     /old_locus_tag="pgaptmp_003626"
     CDS             complement(3960..4781)
                     /gene="cobB"
                     /locus_tag="ACHOSM_RS18130"
                     /old_locus_tag="pgaptmp_003626"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HGU4560054.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4797..5708)
                     /gene="nagK"
                     /locus_tag="ACHOSM_RS18135"
                     /old_locus_tag="pgaptmp_003627"
     CDS             complement(4797..5708)
                     /gene="nagK"
                     /locus_tag="ACHOSM_RS18135"
                     /old_locus_tag="pgaptmp_003627"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HGU4560055.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLCARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5737..6981)
                     /gene="lolE"
                     /locus_tag="ACHOSM_RS18140"
                     /old_locus_tag="pgaptmp_003628"
     CDS             complement(5737..6981)
                     /gene="lolE"
                     /locus_tag="ACHOSM_RS18140"
                     /old_locus_tag="pgaptmp_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HGU4560056.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDKVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6981..7682)
                     /gene="lolD"
                     /locus_tag="ACHOSM_RS18145"
                     /old_locus_tag="pgaptmp_003629"
     CDS             complement(6981..7682)
                     /gene="lolD"
                     /locus_tag="ACHOSM_RS18145"
                     /old_locus_tag="pgaptmp_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HGU4560057.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALDMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(7675..8874)
                     /gene="lolC"
                     /locus_tag="ACHOSM_RS18150"
                     /old_locus_tag="pgaptmp_003630"
     CDS             complement(7675..8874)
                     /gene="lolC"
                     /locus_tag="ACHOSM_RS18150"
                     /old_locus_tag="pgaptmp_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HGU4560058.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            9136..10209
                     /locus_tag="ACHOSM_RS18155"
                     /old_locus_tag="pgaptmp_003631"
     CDS             9136..10209
                     /locus_tag="ACHOSM_RS18155"
                     /old_locus_tag="pgaptmp_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGU4560059.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVVVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            10337..13783
                     /gene="mfd"
                     /locus_tag="ACHOSM_RS18160"
                     /old_locus_tag="pgaptmp_003632"
     CDS             10337..13783
                     /gene="mfd"
                     /locus_tag="ACHOSM_RS18160"
                     /old_locus_tag="pgaptmp_003632"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGU4560060.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALMMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13927..14889
                     /gene="ldtC"
                     /locus_tag="ACHOSM_RS18165"
                     /old_locus_tag="pgaptmp_003633"
     CDS             13927..14889
                     /gene="ldtC"
                     /locus_tag="ACHOSM_RS18165"
                     /old_locus_tag="pgaptmp_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HGU4560061.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRSGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKNAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14972..15229)
                     /gene="bhsA"
                     /locus_tag="ACHOSM_RS18170"
                     /old_locus_tag="pgaptmp_003634"
                     /gene_synonym="ycfR"
     CDS             complement(14972..15229)
                     /gene="bhsA"
                     /locus_tag="ACHOSM_RS18170"
                     /old_locus_tag="pgaptmp_003634"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HGU4560062.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         3820 a         3966 c         3850 g         3642 t
ORIGIN      
        1 gaggctgaat atttcatgcg caaaattgat gaaagtccgt cagcaacaaa gcgtcggggt
       61 atcagattaa aggcatgggt tgatcgatat ctgaaaatac aggacacgcg actgaaaaat
      121 ggagatattg cagctacaac tcacaaagaa aaagcccgaa tggctgcata cctggtttcc
      181 cgtctgggaa accacccatt gaaagaactg gaagtaagag actttgcatt aatactggat
      241 gagtggctgg ataaagacat ggtcagcaca gcgagagtaa atcgtggatt atgggttgat
      301 atttataaag aagcacagca tgcaggggaa gttcctcctg gatggaatcc tccggaggct
      361 acccgtaaac cgatccctaa agtaactaga gccaggctca ccctggaaga ctggcaaaaa
      421 atttacaacg caacgcctga aaaacacttt atccgtaacg caatgcttct tgcgattgtt
      481 actggtcagc gccgtgatga catttgccac atgcgttttt cagatgtgtg gaacgaacac
      541 ttgcatatca cccagggaaa aaccggaatg cgtctggcgt taccgcttac actacgctgt
      601 gatgccattg ggataacgtt aaaagaagtt attgatgggt gccgagacag aatattaagt
      661 ccatatctaa tccatagtcg gcaccagaaa caaccaaagc cgatgagtaa agacaacctg
      721 agcgactact ttgccaaagc acgggatctg gctgggataa ctccaccagc aggaaaaact
      781 ccgccaacat ttcatgaaca acgctccctg tcagaacgac tgtaccgcgc gcagggtatt
      841 gatacaaaaa cattactggg acataaagtc caggcaacca ccgatcgtta taacgatact
      901 cgtggtcagg aatgggttaa attggttgtt tgacgaaata aatgcagctg aaaaatgttg
      961 atattgcttt gctagtgtaa aaaccccggc atcaaccggg gttcggagac taatcttgat
     1021 caggttcctg cttagaataa tgttttccaa caataaatgc cgtaaccaat gcaacaagat
     1081 ctattgaaac gagagttccg gcaagaactg tctcccccat gacgccaaag acagtggcag
     1141 ctaagatgat caatattgcc aaccaaaaag cctttgtttg accatctctg gcaatatcaa
     1201 tgctatcagc cactgtttta tggcgatgag cctgctcttt ttcggtcaac tcaacaagtc
     1261 gatttgcaag cccaggcacc agttgatcat actttttaag cattgaaggt ggcggaacag
     1321 gcccctgaaa atgctggcaa acaatagccc gcacctgcgg actatccaaa accctgttta
     1381 agacctctgg attttcgatt actcgagaaa ccagttcatt atccttttgt tcttcacaag
     1441 tgagtttggt gttctcactt tctttttgat ctggcattgt tcagcttttc aacgttaacg
     1501 gataccattg ctttttggaa atcgcctctg atagccgcag catcctgacg caaagcggaa
     1561 actggattcg ctggcatgcc atttgctgaa acgaccgtcg ggctcagggc tataaccgag
     1621 ccaatggctt gtaacaaagc cacactttta cgcttcatag tcactcctga gttcaatggg
     1681 attcaaaacg gattcaaagt gaatcttgat agctaattct aataccacat tatgtggttt
     1741 ttcaacacga agaaatcaca tcaaaaatct cattcggaaa gcgatatggg taaacaaact
     1801 ggaatacttt tctgtcctag taagctcacc catgtataaa aatgctgtaa aatgcgtggt
     1861 cctgttttgg ggaaaagttt tggagagatt ttggagaaag gaaaaaattg aataaattca
     1921 aaatcctgaa agtgtcccgc cttatgaata aaaaggtgga actcgaatga tcggtcgact
     1981 gcttcgcggc ggttttatga ccgctatcta cgcgtacctg tatatcccaa tcattatttt
     2041 gattgtgaac tcctttaaca gctcgcgctt tggcatcaac tggcagggtt ttaccaccaa
     2101 atggtatagc ctgctgatga acaacgacag cctgttacag gcagcgcagc attcactaac
     2161 aatggcggtg ttttcggcga catttgctac gcttatcggt tcactgacgg cagttgcgct
     2221 gtaccgttat cgctttcgtg gtaagccgtt cgttagcgga atgctgtttg tggtgatgat
     2281 gtcaccagat atcgtgatgg cgatttctct gctggtgctg tttatgctgc tgggtattca
     2341 gcttggcttc tggtcgctgc tgttctcgca tatcaccttc tgcctgccgt ttgtggtggt
     2401 gacggtgtat tcgcgcctga aaggttttga cgtgcggatg ctggaagcgg cgaaagatct
     2461 cggtgccagt gaatttacca ttctgcgaaa aatcattctg ccactggcaa tgccagcggt
     2521 ggcggcgggc tgggtgttaa gctttaccct gtcgatggac gatgtggtag tttcttcgtt
     2581 cgtcaccgga ccaagttatg aaattctgcc gttaaaaatt tattcgatgg tcaaagtcgg
     2641 cgtatcgccg gaagttaacg cgctggcaac catattactg gtgctgtcgc tggtgatggt
     2701 tattgccagc cagcttattg ctcgtgataa aacgaaaggt aacacagggg acgttaaatg
     2761 aaaaaatggt cacgccacct gctcgcggcg ggtgctctgg cactgggcat gagcgccgct
     2821 cacgccgatg acaacaacac gctgtatttc tacaactgga ccgagtacgt gccgccagga
     2881 ctgcttgaac agttcaccaa agaaaccggt attaaggtta tctattcgac ttacgagtcg
     2941 aacgaaacca tgtacgccaa gctgaaaacg tacaaagacg gtgcctatga cctggtggtt
     3001 ccttcaacct attacgtcga taaaatgcgt aaagaaggga tgatccagaa gatcgacaag
     3061 tcgaagttaa cgaacttcag caatctcgat ccagacatgc tcaacaagcc gtttgacccg
     3121 aataacgact actccattcc gtatatctgg ggtgcgacgg cgattggcgt taacggtgat
     3181 gcggtggatc ctaaatctgt caccagttgg gcagatctgt ggaagccaga gtacaaaggt
     3241 agcctgctgc tgaccgacga tgcccgtgaa gtgttccaga tggcgctgcg caaactgggc
     3301 tactccggta acaccaccga tccgaaagag attgaagctg catataacga gctgaaaaaa
     3361 ctgatgccaa acgtcgcagc gtttaactcc gataacccgg ctaacccgta catggaaggc
     3421 gaagttaacc tcggcatgat ctggaacggc tctgctttcg ttgcacgcca ggcgggtacg
     3481 ccaattgacg tggtgtggcc gaaagaaggc ggcattttct ggatggacag cctggcgatc
     3541 ccggcaaatg ccaaaaataa agaaggcgcg ctgaaattga tcaacttcct gctgcgcccg
     3601 gatgtggcaa aacaggttgc cgaaactatc ggttatccaa cgccaaacct tgcggcgcgt
     3661 aagctgttaa gcccggaagt ggcgaacgat aaaacgctct acccggatgc tgaaactatt
     3721 aaaaatggtg aatggcagaa tgacgttggc gcagccagca gcatttatga agagtattat
     3781 cagaagctga aagcaggacg ttaattcagc aaacgggcgg tggtattacc gcccgttttt
     3841 tagttagcaa ggatgtggct aatgagtcag gacagtaaag ttttatcccg ctctttcgtc
     3901 ggtattgtgg tccccttgta ggcctgataa gcgtagcgca tcaggcaatg cttcctgctt
     3961 tataatccct tcagcaactt ttcaacaaac tcaggcacca cctggcttgc cgggccgtaa
     4021 tatttctcgg caaattcatt accgacctga cttggttcga gattcagttc cacggtgtgc
     4081 gcgccatgca gtttcgcttc gtgaacaaac ccagccgccg gataaacatg cccggaagta
     4141 ccaatggcaa tgaaaatatc agccatcgac aacgccatat aaatttcatc catgccgagt
     4201 ggcatttcgc caaaccaaac gacgtgcggg cgcaagggtg ccgggaactg gcaacaatgg
     4261 catttatctt ctggcgtaac gtctccggtc cagtcgagaa cctgaccact ttgcgaacaa
     4321 cgaactttca gcaattcccc atgcatatga atgacattgg tattacctgc gcgttcatgc
     4381 aggttgtcta tattctgcgt taccagcaaa aagcgatcgc cgagggcgtc ttgcagttta
     4441 gccagcgcaa gatgcgcggc gttaggctga atttctggct gctgcagctg tcgacgacgg
     4501 gcgttataaa acgcttgcac cagttcagga tcgcgatcga aaccttccgg cgttgccaca
     4561 tcttcaaccc gatgttcttc ccacaggcca tctgcggcgc gaaaggtacg aatacctgat
     4621 tccgcagaaa ttcctgcccc tgtcagtacg agtactcttg gtttttccat tgcttccggc
     4681 accactttat ctctgaaaaa aatacgctga cgcaaacgct cgcgcaggcg gcgtttattt
     4741 ttacgaaaac gacttaaccg atgaccccga cgcgacagca tagcaacctc tgttgtttaa
     4801 tcggttagat gtaggaaggc cgcaccacgc attcctcccg catcaccgtg gcgcgcgcgt
     4861 tcaatgcgcg gaacacgagc tacaggtaag agatgacgag gcagcctgtc cgccagttgc
     4921 gttgtgattg ccgggaaatt cgataagcca ccaccaatga cgaccaggtc agggtcaacg
     4981 atggtcagga tatttcccag acaaaccgct aataaatcca gataacgctc aacgtgcgcc
     5041 cttgcctgct catcgccttg atcataaagc gcaataattt cgggagcctg caacggttga
     5101 tgataatagt gttgccagag ccacgcaaag ccccgacctg atagataatt ttcgatacaa
     5161 ccaatttgac cacagccgca gcggcgtaac gggaaatcca gccccatcat ggttaacgca
     5221 tcaaccggca gacgcatatg gccaaactcg ccggtaatgt agcttttacc ggtaatcggt
     5281 ttaccgttga aaatcagccc gccgccaacg ccggtgccga gaattaaccc catcaccagt
     5341 ggatattgcg taaattcatc atcccaggct tctgaaaggg caaaacagtt ggcatcgtta
     5401 tcaaggcgta catcgcgatc aagacgtgcg cacaggtcgg cacgcagcgg tttaccgctg
     5461 gcagcgggga cattggcggc atacagcgta ccatcttctg tttccggcat tcccggaata
     5521 ccgatgccaa cagaaccttt acagccaaaa cgttggtcag cttcggctac cagctcgcat
     5581 actgcatcta aaaatgcgtc atagctgtca cgcggtgtcg gcacccgctt ttcccactgc
     5641 aactgccgac cgctatcaaa cacgccaagc gcaatttttg ttccaccaat atcaaacccg
     5701 taatacattg ccgctccttg ttttaatgta ctgcctttac tggccgctaa ggactcgcgc
     5761 agggtcaata ttactggcgc gccgcgccgg ataccaactt gccaaaagac tcagcaacaa
     5821 tgctgtgacc agtacgtaga agacgtccag ccagtgcaat tccgatggca ggaagtcaat
     5881 aaaatagata tcgctggaga ggaactgatg accgatcagc ttttcaatcc gctcaataat
     5941 cggggtaagt tgcagtgaaa caactacgcc gataatcaca ccacacaggc taccgaacag
     6001 ccctgccagc aatccatacc agacaaagat ggcgcgaatt aaaccatctt tcgcccccag
     6061 cgttcttaat actgcgatat cgccactctt gtctttcacc gccatcacta aggtggagac
     6121 gatgttgaaa caggccacgc caatcaccag taccatcgcc agatacataa tggcgcggat
     6181 catctggata tcgcgataca tatagccgta agtaccaatc cagcttttaa tataaacata
     6241 gctgttggtc acttcacccg catcgcgtac cagcttattg gcgttgaaaa catccgtcat
     6301 tttaagggca atacctgaca cgctggaacc catatcaaga tattgttggg catccgccag
     6361 cgggatcatg gcaaaactgt gatcgagttg accactcaac tgcaaaatac cggcaacgtg
     6421 caaacgcaca cgttttggct gcatcagttt atgctcagga ttcgagttgg ggatcataat
     6481 cgacacccaa tcgccctgct tcactttcag cgcatccgcc acgcctttgc cgatgataat
     6541 ttgctgttcg cccgctttaa aattgcgcca ggcatccccc tgaacaaacg agggtaatgc
     6601 gctcagacgc tgttcctgtt gcgggttaac gcccttcacc tggattgcgc gcagattggc
     6661 accactttct accagtccgg tgaaattgat atacggcgca gcggcggcaa tacccggcac
     6721 tttctgcact ttatccagtg cttcctgcca gttagtccat ggctgattta ccgcctcaat
     6781 ttcaccgtgc ggtaccacag caagaatgcg gttattcagt tcgcgttcaa agccgttcat
     6841 cgcgcttaag ccgacgatca ataccgccac gccgagggca atgccaatgg tagaaatgac
     6901 agagattagc gacaccatgc cgccgcgtcg ccgtccgcga ctaaaacgca ggccaatcaa
     6961 taacgataaa ggcatcgcca ttactccgcc cccatcaggc tcagttccgc cgtcagacgt
     7021 ccatcacgca tttctaactg gcggctcatg cgttttgcca gttgcaggtc gtgagtaacc
     7081 accaggaagg cggtgccctg caagcgattc aattctccaa gcaactggaa gatgctgtcg
     7141 gcgttacgcg catcgaggtt accggtaggt tcatccgcca gtaccagacg cgggttattg
     7201 accagcgcac gggcaatcgc cacacgctgg cgttcgccgc cagaaagttc agatgggcgg
     7261 tgattcgcac gatgctccag ccccaccgct tttaacatat caagtgcacg gctgttgatt
     7321 tcagcgggct ttttcttgcc aatcagcagc ggcatagcca cgttttccag ggcggtaaaa
     7381 tccggcagca ggtggtgaaa ctgataaata aagcccagct tctggttgcg cagttctgct
     7441 ttcgccgccg aagacagttt gctcatcggt tgaccgttaa agatcacatc gccggaggtt
     7501 ggtgtatcca gcccgcccag caggtgcagc aaggtacttt taccggaacc agagctaccg
     7561 acgatcgcca tcatttcacc ttcgcccacg ctgaaactga cattgtgcag tacatcggtt
     7621 tgcacactgc cttcctgata gcgtttgcac aggttgtcgc attgcaacag gatcttattc
     7681 ataacgtaaa gcctcagcgg gttgtgtggc ggcagcgcgc catgaagggt aaagcgtaga
     7741 cagcagcgcg atagccatcg ccaccagcgc aataacaatg acctgtaaag gttcgatagc
     7801 caccggcagc gccgcgccat caagcaggac gccgattatc ggcatcagat tatttaactg
     7861 gctggcaagc agtgcgccaa gcgccgcacc gaggatcgca ccgataatcc cggcgctggc
     7921 cccttgcacc ataaagacca tcatgatttg tcgcggagtt aagccttgcg tttgcaggat
     7981 cgctacttcg ccctgcttct ccattaccat cagccctagt gaggtaataa tgttaaacgc
     8041 cgcaacggcg acaatcaggc tcagcagtaa gcccatcata tttttttcca tgcgtacggc
     8101 ctggaacagc tcgcctttac ggtcacgcca gtcctgccat ttgctgcctt caggcagttt
     8161 ttgctgactt aaagagtcaa ctttcagcgg ctcatccagc cacaaacgcc agccggtaat
     8221 attgcctgcc ggataacgca tcaggcgtga ggcatcctca atattcacca gcatttcata
     8281 gccatcgact tcactgttgg cggcgaaagt gccaatcaca ttgaacaggc gctgacttgg
     8341 aatacgcccc atcggcgtga actggctggc agatggcacc atcacgcgga tttgatcacc
     8401 gcgattgacg cccaactgtg aggcaagctg ttcaccgagg atgacattat atttccccgg
     8461 ctcgaggtca gtttgtttca cattgaccag atacggcgtt agcggatctt tttgcgccgg
     8521 atcgataccc agcatcaccc cgaccgccac gctgcgcgcg ctttgcagta ccacatcacc
     8581 ggtagtaata ggtgcgacgc gattaacgcc gtccagtttg actgccgttt ccgggagttg
     8641 ctgcgggtta agagagccat gctcagaaga gagaattgcc tgtggcatca ggccaaggat
     8701 gttgttttgc agctcgcgct caaagccgtt catcactgac aataccgtga ccagcgccat
     8761 caccccgagg gtaatgccga tggtagaaag ccaggagacg aaacgaccga agcgatccgc
     8821 tgcacgccca cgcatgtaac gcaggccaat aaatagagcg acaggttggt acatgaaatc
     8881 cgtctggttg ctgtagcaaa gacacgagta tataagcgaa agcaagatgg gtaaacgtct
     8941 gaactgtaag tgatcgtcag ggctaatctg aaaagccccc gtaaatcaat cggctaaatt
     9001 gccatgtttt atcatcaatc ttgccgcctg atcttaatct gaaaaatcat ccaatacaga
     9061 cagataccca ttacacctcg ggtcataacc agcaggatca accgcatgta aattaaaaaa
     9121 gagcgtgttg tcctgatgaa acaaaaagag ctatggatta accagatcaa agggttatgt
     9181 atttgtctgg tggtgattta tcactcggtc attacctttt atccgcatct gaccactttt
     9241 cagcatccgt tatcggaagt cctgagcaaa tgctggatct atttcaatct ttaccttgcc
     9301 ccctttcgta tgccggtttt tttctttata tccggctatt tgattcgccg ctatatcgac
     9361 agcgtaccgt ggggaaattg tctcgataaa cggatctgga acatcttctg ggtgctggcg
     9421 ctttggggcg tggtgcagtg gctggcgtta agtgcgctga accagtggct ggcacctgag
     9481 cgcgatttaa gcaatgcctc caatgccgct tatgccgatt ccaccggtga gttcctgcac
     9541 gggatgatca ccgccagcac cagcttgtgg tatctgtatg cgttaattgt ctatttcgtg
     9601 gtatgtaaaa tttttagccg tctggcgctg ccactattcg ccttgtttgt actgctgagt
     9661 gtggtggtta atttcgttcc cacgccgtgg tggggaatga acagtgtgat ccgcaatttg
     9721 ccttattaca gccttggcgc atggtttggc gcaacaataa tgacctgtgt taaagaggtg
     9781 ccgttgcgcc gccatctgct gatggcttct ttgcttaccg ttctggcggt cggtgcctgg
     9841 ttgtttacta tctcgctgct gttgtcgctg gtatcgattg tggtgatcat gaagctgttt
     9901 tatcagtacg agcaacgatt cggtatgcgc tccaccagcc tgctgaatgt gattggctcc
     9961 aacaccattg ctatctacac cacccatcgc attctggttg aaatattcag cttaactctg
    10021 cttgcgcaaa tgaacgcagc acgctggtcg ccgcaagtcg aactgacact cctgctggtt
    10081 tatccctttg tcagtttgtt catctgtact gttgcgggct tgctggtaag aaaactttca
    10141 cagcgtgcat tcagcgatct gttgttctcc ccgccttcac tgcccgctgc cgtcagttac
    10201 tctcgttaaa ccatcgcccc agccggggcg attcaatttg ctaaaccatg tcgattacgg
    10261 ataatgaaca gcattgctta tcagagatat gcccccatat gttgaggcat atcctaacga
    10321 gaatctgaca accgttatgc ctgaacaata tcgttatacg ctgcccgtca aagcgggtga
    10381 gcagcgtctg ctgggcgagt taaccggcgc agcctgtgca acgctggtag cggaaattgc
    10441 cgaacgtcac gccggtccgg tggtactcat tgcaccagat atgcaaaatg ctctgcgttt
    10501 gcatgatgaa atcagccagt tcaccgatca gatggtgatg aatctggcgg actgggaaac
    10561 tcttccctac gacagttttt cgcctcatca ggacattatc tcctcgcgcc tttccaccct
    10621 ttaccagcta ccgacgatgc agcgtggcgt actgattgtt ccggtgaata cgcttatgca
    10681 gcgcgtttgc ccacacagtt ttctccacgg tcatgcgctg atgatgaaaa aaggtcagcg
    10741 cctgtcacga gatgcattac gaacccaact ggacagcgcc ggttatcgcc atgttgacca
    10801 ggtgatggag cacggcgaat acgccacgcg cggcgcgttg ctggatctct tcccgatggg
    10861 gagtgagctg ccttatcgtc ttgatttctt tgatgatgaa atcgacagcc tgcgggtgtt
    10921 tgacgtcgac agccagcgca cgctggagga agtagaagcg atcaatctgc tgcccgcgca
    10981 cgaatttccg accgataaag cggcaattga actgttccgc agccagtggc gcgatacctt
    11041 cgaagtgaag cgcgatccgg aacatattta ccagcaagtg agtaaaggca cattacctgc
    11101 cgggatcgag tactggcagc cattgttctt cagcgaacca ctgccgccgc tgttcagtta
    11161 tttccctgcc aataccttgc tcgtgaatac tggcgatctg gaaaccagtg ctgaacgttt
    11221 ccaggctgac acgctggcgc gttttgagaa tcgcggcgtc gatccgatgc gcccgctgtt
    11281 gccaccacaa tcgctctggc tgcgggtgga cgagctcttc tcagagctga aaaactggcc
    11341 ccgggtgcag ctaaaaactg aacatttacc gacaaaagcc gcgaatgcca atttaggttt
    11401 ccagaaactg ccagacctgg ccgttcaggc gcaacaaaaa gcgccgctgg atgcgctgcg
    11461 taagttcctc gagactttcg acggtccggt ggtgttctcg gtagaaagtg aaggtcgccg
    11521 tgaagcgctg ggtgaactgc tcgcacgaat taaaattgct ccgcaacgca ttatgcgtct
    11581 tgatgaagcc agcgaccgtg ggcgttatct gatgattggc gctgccgaac atggttttgt
    11641 cgataaggtg cgtaatctgg cgctgatctg cgaaagcgat ctgctcggtg aacgtgttgc
    11701 ccgccgtcgt caggattctc gccgcaccat caaccccgat acactgatcc gtaaccttgc
    11761 ggagctgcat attggtcagc cggtggtcca tctggagcac ggtgtcgggc gctatgccgg
    11821 aatgaccacg ctcgaagcgg gcggcattac tggcgagtat ttgatgctca cctatgccaa
    11881 cgacgccaaa ctgtatgttc cggtgtcgtc actgcatctg attagccgtt acgcaggtgg
    11941 cgcggaagaa aacgccccgc tgcataaact tggcggcgat gcgtggtcac gcgcgcggca
    12001 gaaagcggcg gaaaaagtgc gtgatgtggc ggcggaattg ctggatatct acgcgcaacg
    12061 cgccgccaaa gagggcttcg cgtttaaaca cgatcgtgag cagtatcagt tgttctgcga
    12121 cagcttcccg tttgaaacca cgccggatca ggcgcaggcc attaatgcgg tacttagcga
    12181 catgtgtcag ccgctggcaa tggatcgtct ggtgtgcggc gatgttggct ttggtaaaac
    12241 agaagtggcg atgcgcgccg ctttcctggc agtagataac cacaagcagg tggcggtgtt
    12301 ggtgcctacc acccttctcg cgcagcagca ttacgacaac ttccgcgacc gtttcgccaa
    12361 ctggccggta cgtatcgaaa tgatctcccg tttccgcagc gccaaagagc agacgcaaat
    12421 ccttgcggaa gtggcggaag ggaaaatcga tattctgatc ggtacgcaca aactgctgca
    12481 aagtgacgtc aagtttaaag atttaggcct gctgattgtc gatgaagaac accgcttcgg
    12541 ggtgcgtcat aaagagcgca ttaaagcgat gcgcgcgaac gtggatattc tgacgcttac
    12601 tgcaacgccg atcccacgca cgctgaatat ggcaatgagc ggaatgcgtg acctgtcgat
    12661 tatcgccacg ccgcccgccc gtcgtctggc agttaaaacc tttgtccgtg agtatgacag
    12721 cctggtggtc cgggaggcga tcctgcgtga aattttgcgc ggggggcagg tttattatct
    12781 ctacaatgat gtggaaaaca tccagaaagc tgccgaacgg ctggcagaac tggtgccaga
    12841 agcgcggatc gccatcggtc acgggcagat gcgcgagcgc gaactggaac gggtgatgaa
    12901 tgatttccat catcaacgtt tcaacgtgct ggtttgtaca accattatcg aaaccgggat
    12961 cgacatcccg acagccaaca ccattatcat tgaacgcgcg gatcacttcg gtctggcgca
    13021 gctgcaccag ttacgcggtc gcgtcggacg ttcacatcat caggcatatg catggctgct
    13081 gacgccgcat ccaaaagcga tgactaccga tgcacaaaaa cgtcttgaag cgattgcctc
    13141 gctggaagat ctcggtgcag gctttgcgct ggcaacgcac gatctggaga tccgcggcgc
    13201 gggtgaactg cttggcgaag aacaaagtgg ctcaatggaa accatcggtt tctcgctgta
    13261 tatggagttg ctggaaaacg ccgtcgatgc actgaaagcc ggacgcgagc cgtcgctgga
    13321 agatctcacc agccagcaaa cagaggtcga gctgcggatg ccgtcgctat tgccagatga
    13381 tttcattcct gacgtgaata cgcgtttgtc gttctataaa cgtattgcca gcgccaaaac
    13441 ggaaaacgaa ctggaagaga tcaaagtcga gcttatcgat cgcttcggcc tgctgccaga
    13501 tccggcgcgt accctgctgg atattgcccg tctgcgccag caagcgcaga aactggggat
    13561 caggaagctg gaaggtaatg agaaaggcgg ggtgatcgaa tttgccgaga agaatcacgt
    13621 taatccggcc tggttgattg gtttgctgca aaaacagccg cagcattacc gccttgatgg
    13681 tccgacgcgc ctgaaattta ttcaggattt gagtgagcgg aaaacgcgta tcgaatgggt
    13741 acgccagttt atgcgtgaac tggaagagaa cgcgatcgct taatggtaaa tcgggggcgt
    13801 ttctccgccc ccatctgcaa catttacaaa ttctttgcac ttccctgcac tatccgacac
    13861 tgtcaccatc cataattcag gcttatctgt ttattacaat aaccttatat ttattatgga
    13921 tttttggtga tgatcaaaac gcgtttttct cgctggctaa cgttttttac gttcgccgct
    13981 gccgtggcgc tggcgctacc ggcaaaagcc aacacctggc cgctgccgcc agcgggcagt
    14041 cgtctggttg gcgaaaacaa atttcatgtg gtggaaaatg acggtggttc tctggaagcc
    14101 atcgccaaaa aatataacgt cggctttctc gctctgttac aggctaaccc cggcgttgat
    14161 ccttacgtac cgcgctcggg cagcgtgtta acgatcccgt tgcaaaccct acttccagac
    14221 gcgccgcgcg aaggcattgt gatcaacatt gcggagctgc gtctctatta ctacccgccg
    14281 ggtaaaaatt cggtaaccgt gtatccaata ggtattggtc agttaggtgg tgacacgctg
    14341 acaccgacaa tggtgaccac cgtttcagac aaacgtgcaa acccaacctg gacgccaacg
    14401 gcaaacatcc gcgcccgtta taaagcacag ggaattgagt tgcctgcggt agtgccggct
    14461 ggaccggata acccaatggg ccatcatgcg attcgtctgg cggcctatgg cggcgtttat
    14521 ttgcttcatg gtacgaacgc cgatttcggc attggcatgc gggtaagttc tggctgtatt
    14581 cgtctgcggg atgacgatat caaaacactc tttagccagg tcaccccagg caccaaagtg
    14641 aatatcatca acactccgat aaaagtctct gccgaaccaa acggtgcgcg tctggttgaa
    14701 gtacatcagc cgctgtcaga gaagattgat gacgatccgc agctgctgcc aattacgctg
    14761 aatagcgcaa tgcaatcatt taaaaatgca gcacaaactg acgctgaagt gatgcaacat
    14821 gtgatggatg tccgttccgg gatgccggtg gatgtccgcc gtcatcaagt gagcccacaa
    14881 actctgtaaa tcctggataa aaaaaatccc cgcgacacgg catgtcgcgg ggaaaaaatc
    14941 agcagtagca ggcattaatg agggttaatg cttatttata aattactgct gttccatgga
    15001 gggtattcgg accggttaca gaagtaatac ggaaagattt tgcgcccatc tcatccgctt
    15061 tttgcgccag ctgctcttcc agcgatccca gatttgtccc cgcgttagca ctgatagtac
    15121 cgactttttg ttgaccttct ggcgttgact gaacttcgac agccgcaaag ctggcaaatg
    15181 acatggagct taaaatcgcc gcagcgatga gggtttttac gtttttcata atagtggcct
    15241 tatgcagatg aatgacgtcg caagcaatta cttaacga
//
LOCUS       DAZKHC020000093        15211 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZKHC020000093 DAZKHC020000000
VERSION     DAZKHC020000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.093"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            158..1447
                     /gene="hpaG"
                     /locus_tag="ACHOSM_RS18175"
                     /old_locus_tag="pgaptmp_003635"
     CDS             158..1447
                     /gene="hpaG"
                     /locus_tag="ACHOSM_RS18175"
                     /old_locus_tag="pgaptmp_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HGU4560063.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            1444..2910
                     /gene="hpaE"
                     /locus_tag="ACHOSM_RS18180"
                     /old_locus_tag="pgaptmp_003636"
     CDS             1444..2910
                     /gene="hpaE"
                     /locus_tag="ACHOSM_RS18180"
                     /old_locus_tag="pgaptmp_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4560064.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2912..3763
                     /gene="hpaD"
                     /locus_tag="ACHOSM_RS18185"
                     /old_locus_tag="pgaptmp_003637"
     CDS             2912..3763
                     /gene="hpaD"
                     /locus_tag="ACHOSM_RS18185"
                     /old_locus_tag="pgaptmp_003637"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HGU4560065.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3773..4153
                     /locus_tag="ACHOSM_RS18190"
                     /old_locus_tag="pgaptmp_003638"
     CDS             3773..4153
                     /locus_tag="ACHOSM_RS18190"
                     /old_locus_tag="pgaptmp_003638"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HGU4560066.1"
                     /translation="MPHFIVECTENIRQEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            4221..5024
                     /gene="hpaH"
                     /locus_tag="ACHOSM_RS18195"
                     /old_locus_tag="pgaptmp_003639"
     CDS             4221..5024
                     /gene="hpaH"
                     /locus_tag="ACHOSM_RS18195"
                     /old_locus_tag="pgaptmp_003639"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HGU4560067.1"
                     /translation="MFDKHTHTLIALRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            5035..5823
                     /gene="hpaI"
                     /locus_tag="ACHOSM_RS18200"
                     /old_locus_tag="pgaptmp_003640"
     CDS             5035..5823
                     /gene="hpaI"
                     /locus_tag="ACHOSM_RS18200"
                     /old_locus_tag="pgaptmp_003640"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HGU4560068.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5996..7372
                     /gene="hpaX"
                     /locus_tag="ACHOSM_RS18205"
                     /old_locus_tag="pgaptmp_003641"
     CDS             5996..7372
                     /gene="hpaX"
                     /locus_tag="ACHOSM_RS18205"
                     /old_locus_tag="pgaptmp_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HGU4560069.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVATLLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7382..8272
                     /gene="hpaA"
                     /locus_tag="ACHOSM_RS18210"
                     /old_locus_tag="pgaptmp_003642"
     CDS             7382..8272
                     /gene="hpaA"
                     /locus_tag="ACHOSM_RS18210"
                     /old_locus_tag="pgaptmp_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HGU4560070.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8523..10085
                     /gene="hpaB"
                     /locus_tag="ACHOSM_RS18215"
                     /old_locus_tag="pgaptmp_003643"
     CDS             8523..10085
                     /gene="hpaB"
                     /locus_tag="ACHOSM_RS18215"
                     /old_locus_tag="pgaptmp_003643"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HGU4560071.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10103..10615
                     /locus_tag="ACHOSM_RS18220"
                     /old_locus_tag="pgaptmp_003644"
     CDS             10103..10615
                     /locus_tag="ACHOSM_RS18220"
                     /old_locus_tag="pgaptmp_003644"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HGU4560072.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQTIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            11008..13158
                     /gene="btsT"
                     /locus_tag="ACHOSM_RS18225"
                     /old_locus_tag="pgaptmp_003645"
     CDS             11008..13158
                     /gene="btsT"
                     /locus_tag="ACHOSM_RS18225"
                     /old_locus_tag="pgaptmp_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HGU4560073.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13208..13411
                     /locus_tag="ACHOSM_RS18230"
                     /old_locus_tag="pgaptmp_003646"
     CDS             13208..13411
                     /locus_tag="ACHOSM_RS18230"
                     /old_locus_tag="pgaptmp_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU4560074.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13422..14378
                     /gene="yjiA"
                     /locus_tag="ACHOSM_RS18235"
                     /old_locus_tag="pgaptmp_003647"
     CDS             13422..14378
                     /gene="yjiA"
                     /locus_tag="ACHOSM_RS18235"
                     /old_locus_tag="pgaptmp_003647"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGU4560075.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DIAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLRK"
     gene            complement(14464..14655)
                     /locus_tag="ACHOSM_RS18240"
                     /old_locus_tag="pgaptmp_003648"
     CDS             complement(14464..14655)
                     /locus_tag="ACHOSM_RS18240"
                     /old_locus_tag="pgaptmp_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HGU4560076.1"
                     /translation="MSMVKHKRGTLYTLDVHQEAELKALVNKSDEDIDYSDIPASGSE
                     AWADATRGNFLPDPGIHAQ"
     gene            complement(14696..>15211)
                     /locus_tag="ACHOSM_RS18245"
                     /old_locus_tag="pgaptmp_003649"
     CDS             complement(14696..>15211)
                     /locus_tag="ACHOSM_RS18245"
                     /old_locus_tag="pgaptmp_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560077.1"
                     /translation="IIYLFTSSYASSLFLSLSNLNITLTPWVEYFWKGMAFMLLFFML
                     MQLIIYFAFLTLGTKLNVYRLFILAGAFIVSTILVAFASKNVEGISYYVLKSTIDFEW
                     RNQIKCGQLNISRSNEKYFGFNTDKYTVFYSNREGKWGFDELKCKKGSDRRDAYSIEN
                     VSEYNVPGWLK"
BASE COUNT         3608 a         4122 c         4072 g         3409 t
ORIGIN      
        1 taacttactg atatttaata ttttattatc tttaaatgta aatagtttgt taattagatc
       61 acatttacat cacttacttt tgccaaacta cgcaatcggg aataaaagta atcattaata
      121 tattaatgat taagatgatc gaatccgagg agtggtaatg aaaggcacta tcttcgccgt
      181 agcgttgaac catcgcagcc aacttgatgc atggcaggaa gcgttccagc aatcccccta
      241 caaagccccg cctaaaactg cggtctggtt tattaaaccg cgcaatacgg tgattggttg
      301 cggtgaaccg attccctttc cacagggtga aaaggtactg agcggtgcga ctgttgcgct
      361 gattgtggga aaaacggcga cgaaagtacg tgaagaagat gcggctgagt acatcgccgg
      421 atatgcgctg gctaacgacg tcagcctgcc ggaagagagc ttttaccgcc cggcaatcaa
      481 agcaaaatgt cgtgatggat tctgccccat tggcgaaacc gtggctctca gcaatgtcga
      541 taatctgacc atctataccg agatcaacgg gcgtcctgcc gatcactgga ataccgccga
      601 tttacaacgt aacgccgcgc agttgctgag cgccctgagc gaatttgcca cactgaatcc
      661 aggcgatgcc attctgctcg gcacgccaca ggcgcgcgtg gaaatacagc caggcgatcg
      721 cgttcgtgtt ctcgcagaag gtttcccgcc gctggaaaat ccggtagtgg acgaacgtga
      781 agtgaccacg cgcaagagct tcccaacgca gccacacccg cacggcacgc tgtttgccct
      841 cggcctgaac tacgccgacc acgccagcga actggaattt aagccaccgg aagaaccgct
      901 ggtgttcctg aaagcgccaa ataccctcac tggcgataac cagacctccg tgcgtccaaa
      961 caatattgaa tacatgcact atgaagcgga gctggtggta gttattggca aacaggcgcg
     1021 taacgtcagc gaagccgatg ccatggatta tgtcgcgggc tacaccgtgt gtaacgacta
     1081 cgccattcgc gactatctgg aaaactacta ccgccctaac ctgcgggtaa aaagccgcga
     1141 cggactgacg ccgatgcttt caaccatcgt gccgaaagag gcgatcccgg acccgcataa
     1201 tctgaccctt cgcaccttcg tcaacggcga gttacgccag caaggcacca ccgccgatct
     1261 gatcttcagc gtgcccttcc tgatcgccta cttaagtgaa tttatgaccc tgaatccggg
     1321 cgacatgatc gccaccggca cacccaaagg cttatctgac gtggtgcctg gcgatgaagt
     1381 agtggtggaa gtagaaggcg tgggccgcct ggtgaaccga attgtgagtg aggatacagc
     1441 gaaatgaaaa aagtaaatca ttggatcaac ggcaaaaatg ttgcaggtaa cgactacttc
     1501 cagaccacca atccggcaac gggtgaagtg ctggcggatg tggcctctgg cggtgaagcg
     1561 gagatcaatc aggcggtagc ggcagcgaaa gaggcgttcc cgaaatgggc caatctgccg
     1621 atgaaagagc gtgcgcgcct gatgcgccgt ctgggcgatc tgatcgacca gaacgtgcca
     1681 gagatcgccg cgatggaaac cgcggacacc ggcctgccga tccatcagac caaaaatgtg
     1741 ttgatcccac gcgcttccca caactttgaa tttttcgcgg aagtctgcca gcagatgaac
     1801 ggcaagacct atccggttga cgacaagatg ctcaactaca cgctggtgca gccggtgggc
     1861 gtttgtgcgc tggtatcgcc gtggaacgta ccgtttatga ccgccacatg gaaggtcgcg
     1921 ccgtgtctgg cgctgggcaa taccgcggta ctgaaaatgt cggaactctc cccgctgacc
     1981 gctgaccgcc tgggtgagct ggcgctggaa gccggtattc cggcaggcgt gctgaacgtg
     2041 gtacagggct acggcgcaac cgcaggggat gcgctggttc gtcatcatga cgtacgtgcc
     2101 gtgtcgttca ccggcggtac ggccaccggg cgcaacatca tgaaaaacgc cgggctgaaa
     2161 aaatactcca tggaactggg cggtaaatcg ccggtgctga tttttgaaga tgccgatatt
     2221 gaacgcgcgc tggacgccgc cctgttcacc atcttctcga tcaacggcga acgctgcacc
     2281 gccggttcgc gcatctttat tcagcaaagc atctacccgg aattcgttaa acgctttgcc
     2341 gaacgcgcca accgtctgcg cgtgggcgat ccgaacgatc cgaataccca ggttggcgcg
     2401 cttattagcc agcagcactg ggaaaaagtc tccggctata tccgtctcgg cattgaagaa
     2461 ggcgcaaccc tgctggcggg cggcccggat aaaccgtccg acctgcctgc gcacctgaaa
     2521 ggcggcaact tcctgcgccc aaccgtgctg gcagacgttg ataaccgtat gcgagtcgcc
     2581 caggaagaga ttttcgggcc ggtcgcctgc ctgctgccgt ttaaagacga agccgaaggc
     2641 ttacgtctgg caaacgacgt ggagtacggc ctcgcgtcgt acatctggac acaggatgtc
     2701 agcaaagtgt tacgcctggc gcgtggcatt gaagctggca tggtgttcgt caacacccag
     2761 aacgtgcgtg acctgcgcca gccatttggc ggcgtaaaag cctccggcac cgggcgtgaa
     2821 ggcggtgagt acagcttcga agtgttcgcg gaaatgaaga acgtctgcat ttccatgggc
     2881 gaccatccaa ttccgaaatg gggagtctga tatgggtaag ttagcgttag ccgccaagat
     2941 cacgcacgta ccgtcgatgt atctctctga actgccaggg aaaaaccacg gttgccgcca
     3001 gggcgcgatc gacgggcata aagagatcag caagcgttgc cgggaaatgg gcgtcgatac
     3061 cattatcgtt ttcgataccc actggctggt caacagtgct tatcacatca actgtgcaga
     3121 ccattttgaa ggcgtctaca ccagcaacga gctgccgcat tttatccgcg atatgaccta
     3181 caactacgag ggcaacccgg agttggggca gcttattgcc gatgaagcct taaagctcgg
     3241 cgtgcgggca aaagcgcaca acattcccag cctgaaactg gaatacggca cgctggtgcc
     3301 gatgcgctac atgaatgaag acaagcactt caaagtggtc tccatttcgg ctttctgcac
     3361 ggttcacgat tttgccgaca gccgcaagct gggcgaagcg attctgaaag cgatcgaaca
     3421 gtacgacggc accgtggcgg tccttgccag cggttcgtta tcgcaccgct ttattgacga
     3481 tcagcgtgcg gaagaaggga tgaacagcta cacccgcgag ttcgaccgcc agatggacga
     3541 gcgcgtggtg aagttgtggc gcgaaggcca gttcaaagag ttctgcaata tgctgccgga
     3601 gtacgccgac tactgctacg gcgaaggcaa tatgcacgac acggtgatgc tgctggggat
     3661 gctcggctgg gataaatacg acggcaaggt ggagtttatc accgagctat tcccaagctc
     3721 tggcaccggt caggttaacg ctgttttccc gctgcccgcg taaggacgtt ttatgccgca
     3781 ctttatcgtg gaatgtactg aaaatattcg tcaagaagcc cgcttacctg agctgtttgc
     3841 cagtgttaac accgcactgg cggcgacggg gatttttccg cttgggggaa tacgtagccg
     3901 ggcacactgg atcgatacct ggcaaatggc tgatggcaag cacgattacg cctttgtgca
     3961 tatgacgcta aaaatcggca gcggacgcag tctggaaagc cgtcaggaag tgggcgaaat
     4021 gctgtttgat ctgattaaaa cgcacttcgc ctcactgatg gagagccgtt acctggcgct
     4081 ttcatttgag attgcagagc tgcacccgac gttgaatttc aaacagaaca acgtacacgc
     4141 gttgtttaaa taaatttgca ccagcttcca ggccggatga taaggcgctg ccgcatccgg
     4201 caatgccaca ggatatcgct atgttcgata aacacaccca caccctgatc gccctgcgtc
     4261 tggatcaggc agaaaaacag cgcgaacaga tccgcgcgat ctcgctggat tacccggaga
     4321 tcaccatcga agacgcctac gccgtacagc gcgaatgggt ccgcctgaaa attgccgaag
     4381 gccgtacgct gaaaggccac aaaatcggcc tgacctcgaa agcgatgcag gccagctcgc
     4441 agatcagcga accagattac ggtgcactgc tggacgacat gttcttccac gatggcagcg
     4501 atatcccgac cgatcgcttt atcgtgccgc gcattgaagt ggagctggct ttcgtgctgg
     4561 caaaaccgct gcgtgggcca aactgcacgc tgttcgacgt ctacaacgcc acggactacg
     4621 tgatcccggc gctggagttg atcgacgctc gctgccacaa catcgatccg gaaacccagc
     4681 gcccgcgcaa agtgttcgac accatttctg ataacgccgc caacgccggg gtgatcctcg
     4741 gcggtcgtcc gatcaaacca gacgaactgg atctgcgttg gatctccgcc ctgatgtatc
     4801 gcaatggcgt gattgaagaa accggcgtcg ccgctggcgt gctgaatcat ccggcaaacg
     4861 gcgtggcctg gctggcgaac aaactcgcac cgtatgacgt acaactggaa gccgggcaaa
     4921 tcattctcgg tggttcgttc acccgcccgg ttccggcgcg taagggcgac accttccacg
     4981 tcgattacgg caatatgggc tccattagct gccgctttgt ttaaggagag aacgatggaa
     5041 aacagcttta aagcggcgct gaaagcaggc cgcccgcaga tcggattatg gctggggctg
     5101 agtagcagct acagcgcaga gttactggcc ggagcaggat tcgactggtt attgatcgac
     5161 ggtgagcacg cgccgaataa cgtgcaaacc gtgctcaccc aactacaggc gattgcgccc
     5221 taccccagcc agccggtagt acgtccgtcg tggaacgatc cggtgcaaat caaacaactg
     5281 ctggacgtcg gcacacaaac cttactggtg ccgatggtac aaaacgccga cgaagcccgt
     5341 gaagcggtac gcgccacccg ttatcccccc gccggtattc gcggtgtggg cagtgcgctg
     5401 gcccgagcct cgcgctggaa tcgcattcct gattacctgc aaaaagccaa cgatcaaatg
     5461 tgcgtgctgg tgcagatcga aacgcgtgag gcaatgaaga acttaccgca gattctggac
     5521 gtggaagggg tcgacggcgt gtttatcggc ccggcggatc tgagcgccga tatgggttat
     5581 gccggtaatc cgcagcaccc ggaagtacag gccgccattg agcaggcgat cgtgcagatc
     5641 cgcgaagcgg gcaaagcgcc ggggatcctg atcgccaatg agcaactggc aaaacgctat
     5701 ctggaactgg gcgcactgtt tgtcgccgtc ggcgttgaca ccaccctgct cgcccgcgcc
     5761 gctgaagcgc tggcagcacg gtttggtgct caggccaccg ccgtgaagcc cggcgtgtat
     5821 taacgccgga tgcgctgcgc ttatccggcc tacacgcacc aaaccgtagg ccggataagg
     5881 cgtttacgcc gcatccggca aaaagccgta ccaaatcgcg gaccggataa ggcgtttgtg
     5941 cgcatccgcc ataaaccttg tcgtacccta caaaaatccc attagaggaa gaaaaatgag
     6001 cgacacctca cctgccatcc cggagagtat cgatccggcg aatcagcata aagcgctgac
     6061 tgccggacaa caggcggtta ttaagaagct atttcgccgc ctgatcgtct ttctgttcgt
     6121 gctgtttatc ttctcgttcc ttgatcgcat caacatcggc tttgccggac tcacgatggg
     6181 acgcgacctc ggtctgagcg ccaccatgtt tggcctcgcc accaccttgt tctacgccgc
     6241 ttacgtcatc ttcggcattc ccagcaacat tatgctgagt attgtcggtg cacggcgctg
     6301 gatcgccacc atcatggtgc tttggggcat cgcctctact gccaccatgt ttgccactgg
     6361 ccccaccagc ttatacgtac tgcgtatact ggttggcatt accgaagccg gctttctgcc
     6421 tggcattctg ctgtatttaa ccttctggtt tccggcctat ttccgcgccc gtgccaacgc
     6481 cttgtttatg gtagcaatgc cggtaacgac agcgttgggc tcgatcgttt ccggctacat
     6541 tttgtcgctg gatggcgtaa tggcattaaa aggctggcag tggctgtttt tgctggaagg
     6601 cttcccgtcg gtattactcg gcgtcatggt gtggttctgg cttgatgact caccggacaa
     6661 agctaagtgg ctgacgaaag aagacaaaaa atgcctgcaa gagatgatgg ataacgatcg
     6721 tctgacgctg gttcagccag agggagccat cagccaccac gccatgcaac aacgcagcat
     6781 gtggcgggag atcttcactc cggtggtgat gatgtatacc ctggcgtatt tctgcctgac
     6841 caacacactt agtgcgatca gcatctggac accgcagatc ctgcaaagct ttaatcaggg
     6901 cagcagtaat atcaccatcg gcctgctggc cgccgtaccg cagatttgta ccattctcgg
     6961 gatgatctac tggagccgtc actcagatcg ccgccaggaa cgaaggcatc acaccgccct
     7021 tccttatttg ttcgctgccg ctggttggtt actggcttcg gcaactgatc acaacatgat
     7081 ccagatgctg gggatcatta tggcttcgac cggatcattc agcgcaatgg cgattttctg
     7141 gacaacaccg gatcagtcca tcagcctgcg ggcacgagcg atcggtattg cggtgatcaa
     7201 cgccactggc aacattggtt cagcgttaag tccgtttatg atcggctggt tgaaagatct
     7261 gaccggcagc tttaacagtg gattgtggtt tgttgccacg ctgctggtga ttggtgcggg
     7321 gattatctgg gcgattccaa tgcagtcctc ccgtccgcga gcgaccccgt aaggaacgac
     7381 gatgtgtgac cgtcagattg ccaatattga tatcagcaaa gagtacgatg aaagcctggg
     7441 cacggacgat gtgcattatc agtcgttcgc ccgcatggcg gcattttttg gccgccatat
     7501 gctgccacat cgccacgaac agtactttca gatgcatttc ctcaatagcg gacagattga
     7561 gctacagctt gacgatcatc gctactcggt ggaagcgccc ctgtttgtcc tgacgccacc
     7621 gtcagtacct catgcgttta ttacggagtc tgacgccgac ggtcatgtgt tgacggtacg
     7681 ggaagatctg atctggcccc tgctggaagt tctttatccg ggcactcggg aaaccttcgg
     7741 cctgccgggg atttgcctgt cactggcaga taaacccgac gaactggcgg cgctggaaca
     7801 ctattggcaa ctgatagagc gggaatcggt ggaacaactg cctggacggg aacacaccct
     7861 gacgttactg gcacaggcag tgttcaccct actgctgcgt aacgcaaaac tcgacgacca
     7921 tgccgccagc gggatgcgcg gagaattaaa actgttccag cgttttaata tgctgattga
     7981 aagccatttt catcagcact ggacagtacc ggattacgct aacgaactgc atatcaccga
     8041 atcacgactc acggacatct gccgtcgctt tgccaaccgt ccgccaaaac ggttgatttt
     8101 cgacaggcag ctgcgagaag ccaagcggct gctgctgttt tctgataacg ccgtgaacaa
     8161 tattgcctgg caactcggtt ttaaggatcc ggcttatttt gcgcgctttt ttaatcgctt
     8221 agtcggttgc tcgcccagtg cttatcgcgc caaaaaagta cctgtgacgt gaaaatccct
     8281 tagccttaac gggaaaccag gcaccacctg ctattcccct ttctccgcct ggagcaaggg
     8341 gatttagtaa cgtcaaaaat cgctaaaagc gacctcgatc acaaaaccgt acccgacctg
     8401 aaaagtacca gccattgtcc caaaagtctc ttcccgtaat cgcccctgca agcttcaatt
     8461 gaataacaaa aacaacacac aaaattaaca atcgtattcc gattaatact gtagaggtcg
     8521 acatgaaacc agaagatttc cgcgccagta cccaacgtcc gttcaccggg gaagagtatc
     8581 tgaaaagcct gcaggatggt cgcgagatct atatctatgg cgagcgagtg aaagacgtca
     8641 ctactcatcc ggcatttcgt aatgcggcag cgtctgttgc ccaactgtac gacgcgctac
     8701 acaaaccgga gatgcaggac tctctgtgct ggaacaccga caccggcagc ggcggctata
     8761 cccataaatt cttccgcgtg gcgaaaagtg ccgacgacct gcgccagcaa cgcgatgcca
     8821 tcgctgagtg gtcacgcctg agctatggct ggatgggccg taccccagac tacaaagctg
     8881 ctttcggttg cgcactgggc gcgaatccgg gcttttacgg tcagttcgag cagaacgccc
     8941 gtaactggta cacccgtatt caggaaactg gcctctactt taaccacgcg attgttaacc
     9001 caccgatcga tcgtcatttg ccgaccgata aagtaaaaga cgtttacatc aagctggaaa
     9061 aagagactga cgccgggatt atcgtcagcg gtgcgaaagt ggttgccacc aactcggcgc
     9121 tgactcacta caacatgatt ggcttcggct cggcacaagt gatgggcgaa aacccggact
     9181 tcgcactgat gttcgttgcg ccaatggatg ccgatggcgt gaaattaatc tcccgcgcct
     9241 cttatgagat ggtcgcgggt gctaccggct cgccatacga ctacccgctc tccagccgct
     9301 tcgatgagaa cgatgcgatt ctggtgatgg ataacgtgct gatcccatgg gaaaacgtac
     9361 tgatctaccg cgatttcgat cgctgccgtc gctggacaat ggaaggcggt tttgcccgta
     9421 tgtatccgct gcaagcctgt gtgcgcctgg cagtgaaatt agacttcatt acagcactgc
     9481 tgaaaaaatc actcgaatgt accggcaccc tggagttccg tggtgtacag gccgatctcg
     9541 gtgaagtggt ggcgtggcgc aacaccttct gggcattgag tgactcgatg tgttctgaag
     9601 cgacgccgtg ggtcaacggg gcttatttac cggatcatgc cgcactgcaa acctatcgcg
     9661 tactagcacc aatggcctac gcgaagatca aaaacattat cgaacgcaac gttaccagtg
     9721 gcctgatcta tctcccttcc agtgcccgtg acctgaacaa tccgcagatc gaccagtatc
     9781 tggcgaagta tgtgcgcggt tcgaatggta tggatcacgt ccagcgcatc aagatcctca
     9841 aactgatgtg ggacgccatt ggcagcgagt ttggtggtcg ccacgaactg tatgaaatca
     9901 actactccgg tagccaggat gagattcgcc tgcagtgtct gcgccaggca caaagctccg
     9961 gcaatatgga caagatgatg gcgatggttg atcgctgcct gtcggaatac gaccagaacg
    10021 gctggactgt gccgcacctg cacaacaacg acgatatcaa catgctggat aagctgctga
    10081 aataacgcag caggaggtta agatgcaatt agatgaacag cgcctgcgct ttcgtgacgc
    10141 gatggccagc ctgtcggcag cggtaaatat tatcaccacc gagggcgacg ccggacaatg
    10201 cgggattacg gcaacggccg tctgctcggt cacggataca ccaccatcgc tgatggtgtg
    10261 cattaacgcc aacagtgcga tgaacccggt ttttcagggc aacggtaagt tgtgcgtcaa
    10321 cgtcctcaac catgagcagg aactgatggc acgccacttc gcgggcatga caggcatggc
    10381 gatggaagag cgttttagcc tctcatgctg gcaaaaaggt ccgctggcgc agccggtgct
    10441 gaaaggttcg ctggccagtc ttgaaggtga gatccgcgat gtgcagacaa ttggcacaca
    10501 tctggtgtat ctggtggaga ttaaaaacat catcctcagt gcagaaggtc acggacttat
    10561 ctactttaaa cgccgtttcc atccggtgat gctggaaatg gaagctgcga tttaagctcc
    10621 agccccttca cgcattcgtc gaaggggcat ttttattgtg atctcaatca catatccacc
    10681 cgatgaataa ttcttgccca accgctcacc gccattcctg accgcttacg tccctaaaat
    10741 aaccactcag ttatttacct tactttacgc gcgcgtaact ctggcaacat cactgcagga
    10801 tagcggtcaa tttacctcct caaacgcaac gcaaacctag aacggcttcg gcctatgatt
    10861 aacaaattac tctatgaatt ttggtttgca ggaaggcagc aagtgagtga atccccggga
    10921 gcttacaaca gtaagtgaca ggggtgaacg aacgcaactg ccgcacctgt aagccaaaag
    10981 acgacgagta aaatgccagg ttttactatg gatacgaaaa aactattcaa gcacatacct
    11041 tgggtgattc tcggaatcat cggtgcattc tgcctcgcgg tagttgcatt acgtcggggg
    11101 gagcacgtca gcgccctgtg gatcgtggtc gcctctgtgt cggtgtatct ggtggcgtat
    11161 cgctactaca gtctgtacat cgcccagaag gtgatgaaac tcgaccccac gcgcgcgacg
    11221 cctgcggtta ttaacaacga cggtctgaac tacgttccga ccaaccgtta cgtgttgttt
    11281 ggtcaccact tcgccgctat cgccggtgct ggtccgctgg tcggtccggt tctcgccgca
    11341 cagatgggct acctgcctgg cacgctgtgg ctgctggcgg gggtagtact ggccggtgcg
    11401 gttcaggact ttatggtgct gtttatctcc tctcgccgta acggcgcatc tctcggtgag
    11461 atgataaaag aagagatggg accagtaccg gggactatcg cgctgtttgg ctgtttctta
    11521 atcatgatca tcatccttgc cgtcctggcg ctgattgtgg ttaaagccct ggccgaaagt
    11581 ccgtggggtg tcttcaccgt ttgctcaacc gtaccgattg cgctgtttat gggtatctac
    11641 atgcgcttta tccgtccggg gcgtgtgggt gaagtctctg tcattggtat cgtgctgctg
    11701 gttgcctcta tctacttcgg tggcgtgatt gctcacgatc cgtactgggg tccggcactg
    11761 acctttaaag acaccaccat taccttcgcg ctgattggct atgcgtttgt ttccgcattg
    11821 ctgccggtgt ggctgatcct cgcaccgcgc gactatctgg caaccttcct gaaaatcggc
    11881 gttatcgtcg gcctggcgct gggtatcgtg gtgctgaacc cggaactgaa aatgcctgcg
    11941 atgacccagt acattgacgg tactggcccg ctgtggaaag gtgctctgtt cccgttcttg
    12001 ttcattacca tcgcctgtgg tgcggtatct ggcttccacg cactgatctc ttccggtacg
    12061 acgccgaaac tgctggctaa tgaaaccgac gcgcgtttca ttggctacgg cgcaatgctg
    12121 atggagtcct tcgtggcgat tatggcgctg gttgctgcgt ccatcatcga accgggtctt
    12181 tacttcgcga tgaatacccc gcctgctggc cttggcatca ccatgcctaa cctgcatgaa
    12241 atgggtggcg agaacgcgcc gatcatcatg gcgcagctga aagacgttac cgcacacgcg
    12301 gcagcgaccg tcagctcctg gggcttcgtg atttcgccag agcagatcct gcaaaccgcg
    12361 aaagacattg gtgagccttc tgtcctgaac cgtgcaggtg gtgcgccaac gctggcggta
    12421 ggtatcgctc acgtgttcca caaagtgctg ccgatggctg acatgggctt ctggtatcac
    12481 ttcggtattc tgttcgaagc cctgttcatc ctgaccgcgc tggatgcggg tactcgttct
    12541 ggccgcttta tgctgcaaga cctgctgggt aacttcatcc cgttcctgaa aaaaaccgac
    12601 tctctggttg ccggtatcat cggtactgcg ggctgtgtgg gtctgtgggg ctacctgctg
    12661 tatcagggcg tggtcgatcc gctgggcggc gttaagagcc tgtggccgct gttcggtatt
    12721 tccaaccaga tgctggcagc cgtagcgctg gtactgggca ccgttgtgct gattaagatg
    12781 aagcgcaccc aatacatctg ggtaactgtt gttccggctg tatggctgct tatctgcacc
    12841 acctgggcgc tgggtctgaa actgttcagc accaacccgc agatggaagg cttcttctac
    12901 atggcaagcc agtacaaaga gaagattgct aacggtactg acctgacggc gcagcagatt
    12961 gccaacatga accacatcgt tgtgaacaac tacaccaacg caggcctgag tattctgttc
    13021 ctgattgtgg tgtacagcat catcttctac ggtttcaaaa cctggcttgc ggtgcgtaac
    13081 agcgacaaac gtactgacaa agaaacaccg tacgttccaa tcccggaagg cggcgtgaag
    13141 atctcttcgc accactaacc gtgtttagcc ccgcttcggc ggggctttgt tcttatcaga
    13201 gtgaactatg tttggtaact taggacaggc aaaaaaatat ctcggccagg cggcgaagat
    13261 gttgattggc attccagact acgacaacta cgtcgagcat atgaagacca accatcccga
    13321 caagccgtac atgagctatg aagaattctt ccgcgaacgc cagaacgcac gctacggcgg
    13381 cgatggtaaa ggcggtatgc gctgttgtta atggagagag catgaacccg attgcagtta
    13441 ccctactcac cggtttttta ggcgcgggca aaaccaccct gctgcgccat attcttaatg
    13501 aacaacacgg ctacaagatt gccgtgattg aaaacgaatt cggcgaagtc tccgttgatg
    13561 atcaattgat tggtgaccgc gccacgcaga tcaaaacgct gaccaacggc tgcatctgct
    13621 gttcgcgctc caacgagctg gaggacgcgc tactcgacct gctggacaat ctcgacaagg
    13681 gcaatattca gttcgatcgt ctggtcattg aatgcaccgg catggccgat cccggcccga
    13741 ttattcagac ctttttctcc catgagattt tatgccagcg ctacctgctg gacggggtga
    13801 ttgcgctggt ggatgcggta catgccgatg agcagatgaa ccagttcacc atcgcccagt
    13861 cccaggttgg ctacgccgac cgtattctgc tgactaaaac cgacatcgca ggtgaagcag
    13921 aaaaactgcg cgaacgcctg gcacgcatca acgcccgcgc accggtctac accgtcaccc
    13981 acggcgacat cgacctgggc ctgctgttca acacgaacgg ttttatgctg gaagagaacg
    14041 tcgtcagcac caaaccgcgt ttccacttta tcgcggataa acaaaacgat atttcgtcga
    14101 ttgtggtgga actggattac ccggtagata tcagcgaagt ttcccgcgtg atggaaaacc
    14161 tgctgctgga gtcggcagat aaactgctgc gttacaaagg gatgctgtgg attgacggcg
    14221 aacctaaccg cctgctgttc cagggcgtcc agcgcctcta cagcgccgac tgggaccgcc
    14281 cgtggggcga tgaaaaaccg catagcacga tggtgtttat cgggattcag ctgccggagg
    14341 atgagattcg ggcagcgttt gcggggttga ggaagtaatc gtgaaaggtt gagtgtaaag
    14401 caacccagtg ccccacttag cagtgagggg gaaacagaac gacgtgctcc cctccgctaa
    14461 gttttattgc gcgtgaatac ctggatctgg caaaaaattg ccgcgtgtgg cgtcagccca
    14521 tgcttcgcta ccagacgcag gaatgtcgct gtaatcaata tcttcgtcgg atttattaac
    14581 caaagcttta agttcagctt cttgatgcac atcaagtgtg tagagtgtgc cccgtttatg
    14641 tttaaccatg ctcatttatg accagaacct ttactatata atttataaag atgaattatt
    14701 tcagccatcc aggcacgtta tattctgaga cattctcaat ggaatatgca tctctcctgt
    14761 cagaaccttt cttacatttc aactcatcaa aaccccattt accctctcta ttggaataaa
    14821 atacagtata tttatcagta ttaaacccaa aatacttttc attcgaacgt gaaatattca
    14881 actggccaca tttaatctga ttacgccatt caaaatctat ggtgcttttt aaaacgtagt
    14941 acgaaatacc ttctacattt tttgaagcaa aagccactaa aatagtagat acgataaatg
    15001 ctccggcaag tataaataac ctatacacat ttaatttagt ccccagagtt aaaaaagcaa
    15061 aatagataat aagttgcatt aacatgaaga aaagcaacat aaaagccatc cctttccaga
    15121 aatactcaac ccatggagtt agcgtgatgt ttagattact caaacttaaa aacaaactgg
    15181 aagcataact acttgtgaat aaatatataa t
//
LOCUS       DAZKHC020000094        15164 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZKHC020000094 DAZKHC020000000
VERSION     DAZKHC020000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.094"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            234..569
                     /locus_tag="ACHOSM_RS18250"
                     /old_locus_tag="pgaptmp_003650"
     CDS             234..569
                     /locus_tag="ACHOSM_RS18250"
                     /old_locus_tag="pgaptmp_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGU4560078.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            729..1172
                     /gene="yjdN"
                     /locus_tag="ACHOSM_RS18255"
                     /old_locus_tag="pgaptmp_003651"
     CDS             729..1172
                     /gene="yjdN"
                     /locus_tag="ACHOSM_RS18255"
                     /old_locus_tag="pgaptmp_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HGU4560079.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1305..2093
                     /gene="phnC"
                     /locus_tag="ACHOSM_RS18260"
                     /old_locus_tag="pgaptmp_003652"
     CDS             1305..2093
                     /gene="phnC"
                     /locus_tag="ACHOSM_RS18260"
                     /old_locus_tag="pgaptmp_003652"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HGU4560080.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2118..2995
                     /gene="phnD"
                     /locus_tag="ACHOSM_RS18265"
                     /old_locus_tag="pgaptmp_003653"
                     /pseudo
     CDS             2118..2995
                     /gene="phnD"
                     /locus_tag="ACHOSM_RS18265"
                     /old_locus_tag="pgaptmp_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
     gene            3101..3880
                     /gene="phnE"
                     /locus_tag="ACHOSM_RS18270"
                     /old_locus_tag="pgaptmp_003654"
     CDS             3101..3880
                     /gene="phnE"
                     /locus_tag="ACHOSM_RS18270"
                     /old_locus_tag="pgaptmp_003654"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HGU4560081.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3901..4626
                     /gene="phnF"
                     /locus_tag="ACHOSM_RS18275"
                     /old_locus_tag="pgaptmp_003655"
     CDS             3901..4626
                     /gene="phnF"
                     /locus_tag="ACHOSM_RS18275"
                     /old_locus_tag="pgaptmp_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HGU4560082.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLPVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4627..5079
                     /gene="phnG"
                     /locus_tag="ACHOSM_RS18280"
                     /old_locus_tag="pgaptmp_003656"
     CDS             4627..5079
                     /gene="phnG"
                     /locus_tag="ACHOSM_RS18280"
                     /old_locus_tag="pgaptmp_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HGU4560083.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5076..5660
                     /gene="phnH"
                     /locus_tag="ACHOSM_RS18285"
                     /old_locus_tag="pgaptmp_003657"
     CDS             5076..5660
                     /gene="phnH"
                     /locus_tag="ACHOSM_RS18285"
                     /old_locus_tag="pgaptmp_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HGU4560084.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            5660..6724
                     /gene="phnI"
                     /locus_tag="ACHOSM_RS18290"
                     /old_locus_tag="pgaptmp_003658"
     CDS             5660..6724
                     /gene="phnI"
                     /locus_tag="ACHOSM_RS18290"
                     /old_locus_tag="pgaptmp_003658"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HGU4560085.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPYFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6717..7562
                     /gene="phnJ"
                     /locus_tag="ACHOSM_RS18295"
                     /old_locus_tag="pgaptmp_003659"
     CDS             6717..7562
                     /gene="phnJ"
                     /locus_tag="ACHOSM_RS18295"
                     /old_locus_tag="pgaptmp_003659"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HGU4560086.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHLFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7559..8317
                     /gene="phnK"
                     /locus_tag="ACHOSM_RS18300"
                     /old_locus_tag="pgaptmp_003660"
     CDS             7559..8317
                     /gene="phnK"
                     /locus_tag="ACHOSM_RS18300"
                     /old_locus_tag="pgaptmp_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HGU4560087.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8494..9174
                     /gene="phnL"
                     /locus_tag="ACHOSM_RS18305"
                     /old_locus_tag="pgaptmp_003661"
     CDS             8494..9174
                     /gene="phnL"
                     /locus_tag="ACHOSM_RS18305"
                     /old_locus_tag="pgaptmp_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HGU4560088.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9171..10307
                     /gene="phnM"
                     /locus_tag="ACHOSM_RS18310"
                     /old_locus_tag="pgaptmp_003662"
     CDS             9171..10307
                     /gene="phnM"
                     /locus_tag="ACHOSM_RS18310"
                     /old_locus_tag="pgaptmp_003662"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HGU4560089.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKI
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10307..10864
                     /gene="phnN"
                     /locus_tag="ACHOSM_RS18315"
                     /old_locus_tag="pgaptmp_003663"
     CDS             10307..10864
                     /gene="phnN"
                     /locus_tag="ACHOSM_RS18315"
                     /old_locus_tag="pgaptmp_003663"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HGU4560090.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10851..11285
                     /gene="phnO"
                     /locus_tag="ACHOSM_RS18320"
                     /old_locus_tag="pgaptmp_003664"
     CDS             10851..11285
                     /gene="phnO"
                     /locus_tag="ACHOSM_RS18320"
                     /old_locus_tag="pgaptmp_003664"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HGU4560091.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11287..12045
                     /gene="phnP"
                     /locus_tag="ACHOSM_RS18325"
                     /old_locus_tag="pgaptmp_003665"
     CDS             11287..12045
                     /gene="phnP"
                     /locus_tag="ACHOSM_RS18325"
                     /old_locus_tag="pgaptmp_003665"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HGU4560092.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKYPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12192..12521)
                     /gene="yjdP"
                     /locus_tag="ACHOSM_RS18330"
                     /old_locus_tag="pgaptmp_003666"
     CDS             complement(12192..12521)
                     /gene="yjdP"
                     /locus_tag="ACHOSM_RS18330"
                     /old_locus_tag="pgaptmp_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HGU4560093.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12590..12868)
                     /locus_tag="ACHOSM_RS18335"
                     /old_locus_tag="pgaptmp_003667"
     CDS             complement(12590..12868)
                     /locus_tag="ACHOSM_RS18335"
                     /old_locus_tag="pgaptmp_003667"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HGU4560094.1"
                     /translation="MGGHVAGTAGFCVAVPAFYSWSMSEVIDKDTRSSERTDYPHYAS
                     EVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            13159..15144
                     /gene="yjcS"
                     /locus_tag="ACHOSM_RS18340"
                     /old_locus_tag="pgaptmp_003668"
     CDS             13159..15144
                     /gene="yjcS"
                     /locus_tag="ACHOSM_RS18340"
                     /old_locus_tag="pgaptmp_003668"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HGU4560095.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANVKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPAAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         3441 a         4493 c         4172 g         3058 t
ORIGIN      
        1 aaaatatcat gtgaataaaa aaaagaacaa gtagagcatt aacattatct taaataataa
       61 atagaggcaa aaagattatt ttctttttgc gtttcctttc aaatgaaaac gatcgtcgtc
      121 taaaatcagc agtacccccg acaaactcag ggattttgtg tataattgcg gcctttttcg
      181 gcaatctgcc gttttttggc gcttttgccc tgctgacttt tgaggaaatc cacatgtcat
      241 taccacactg cccaaaatgc aactccgaat acacttacga agataacggc atgtacatct
      301 gcccggaatg tgcctacgaa tggaacgacg cagaacctgc acaggaaagc gacgagctga
      361 tcgttaaaga tgctaacggc aatctgctgg ctgacggcga cagcgttacc atcattaaag
      421 atctgaaggt gaaaggtagc tcttcgatgc tgaaaattgg caccaaagtg aaaaacatcc
      481 gcctggttga aggcgaccat aacatcgatt gcaaaatcga cggttttggt ccgatgaaac
      541 tgaaatctga gtttgtgaaa aagaactgat tgtattgtga tcggtaagcc ggataaggcg
      601 ctcgcgccgc atccggcaac gatgccgact gcctgatgcg acgctgaccg cgtcttatca
      661 ggcttccctc tcatctgtat agatttcgaa ctacacttaa ctggcttctc ttaactgagg
      721 tcaccatcat gccgttaagt ccctacctct cttttgccgg taactgtgcc gacgcaattg
      781 cttattatca acgtacgttg ggcgctgaac tgctctataa aatcagcttc ggcgaaatgc
      841 caaaatcagc gcaggacagc gccgagaact gcccttccgg aatgcaattt cccgataccg
      901 ccatcgccca cgccaatgtg cgcattgccg gaagcgacat catgatgagc gatgccatgc
      961 cgtcaggaaa agccagctac tccggcttta cgctggtgct cgattcgcaa caggtcgaag
     1021 aaggaaaacg ctggtttgac aatcttgccg ctaacggaaa aatcgaaatg gcctggcagg
     1081 aaactttctg ggcgcatggc tttggcaaag tcaccgataa atttggcgta ccgtggatga
     1141 ttaatgtcgt caaacaacaa ccaacgcaat aacccgccgg gaggcccacc ctcccgcact
     1201 gtcatcgaat tcccgttaac tcttcatctg ttagtcactt ttaattaacc aaatcgtcac
     1261 aataatccgc cacgatggag ccactttttt agggaggctg catcatgcaa acgattatcc
     1321 gtgtcgagaa gctcgccaaa accttcaatc agcatcaggc gctgcatgcg gttgatctga
     1381 acattcatca cggtgaaatg gtggctctgc ttgggccgtc gggttccggc aaatccaccc
     1441 ttttacgtca cttaagcggt ttgattaccg gcgataaatc cgccggcagc catatcgagc
     1501 tgctgggccg cacagtccag cgcgaaggcc gtctggcgcg cgatatccgc aaaagccgcg
     1561 ccaacaccgg ctacatcttc caacaattca acctggtgaa ccgcctgagc gtactggaga
     1621 acgtgctgat tggcgcgctc ggcagcacgc cgttctggcg cacctgtttt agctggttta
     1681 cccgcgagca gaaacaacgc gcgttacagg cgctgacccg cgttggcatg gtgcattttg
     1741 cccatcaacg cgtttccacc ctctccggcg gacagcagca gcgtgtggcg attgcccgcg
     1801 cgctgatgca gcaggcgaag gtgattctgg ccgatgaacc catcgcctcg ctggacccgg
     1861 aatccgcccg catcgtgatg gacaccctgc gcgacatcaa tcagaacgac ggcatcaccg
     1921 tggtcgtcac gctgcatcag gtggattacg ccctgcgcta ctgcgaacgc atcgtcgccc
     1981 tgcgccaggg gcacgttttc tacgacggca gcagccaaca gtttgataac gaacgttttg
     2041 accatctcta ccgcagcatt aatcgcatcg aagagaacgc gaaagctgcc tgacatcccc
     2101 atcattgagg aaaacgaatg aacgctaaga taattgcctc gctggccttc accagcatgt
     2161 tcagcctcag caccctgtta agcccggcac acgccgaaga gcaggaaaag gcgctgaatt
     2221 tcggcattat ttcaacggaa tcacagcaaa acctgaaacc gcaatggaag cggtggatcg
     2281 cgccaacggc caggtctttg cccagacggt cgcggcggat ggatcgccgg gttactggag
     2341 cgtgttgatc gtcaacaaag acagcccgat caacaacctg aacgatctgc tggcgaagcg
     2401 gaaagatctc accttcggca atggcgatcc taactccacc tctggcttcc tcgtccccgg
     2461 ttactacgtc tttgccaaaa acaatatctc cgccagcgac tttaagcgca ccgtcaacgc
     2521 tgggcatgaa accaacgcgc tggccgtcgc caacaagcag gtggatgttg ccaccaacaa
     2581 caccgaaaac ctcgacaagc tgaaaacctc cgcgccagag aagctgaaag aactgaaggt
     2641 gatctggaag tcgccgctga tcccaggcga tccgatcgtc tggcgcaaga atctctccga
     2701 aaccaccaaa gacaagatct acgacttctt tatgaactac ggaaaaacgc cggaagagaa
     2761 agcggtgctg gaacgcctgg gctgggcccc gttccgcgcc tccagcgacc tgcaactggt
     2821 gccgattcgc cagctcgcac tgtttaaaga gatgcagggc gtaaaaagca ataaaggact
     2881 gaatgagcag gacaagctgg cgaaaaccac cgagattcag gcgcagctgg atgacctaga
     2941 ccgcctgaac aacgcgttaa gcgcgatgag ttcggtgagt aaagcggtgc agtaaatcgt
     3001 aggtcggata agacgccccg gcgtcgcatc cgacaatgtg caggcgttga tgccggatgc
     3061 ggtgcaagca ccttatccgg cctacagacc ggagccaaac atgcaaacca tcaccatcgc
     3121 cccacccaag cgtagctggt tctcgcttct gagctgggcc gttgttctcg ccgtgctggt
     3181 cgtctcgtgg cagggcgcgg aaatggcccc gcttacgctg attaaagacg gcggcaacat
     3241 ggcaaccttc gctgccgact tcttcccgcc cgatttcagc cagtggcagg attacctcac
     3301 cgaaatggcc gtcacgctgc aaatcgccgt ctggggcacc gcgctggcag tggttctctc
     3361 catccccttc ggtctgatga gcgccgaaaa cctggtgccg tggtgggttt accagcccgt
     3421 tcgccgcctg atggatgcct gccgcgccat taacgaaatg gtcttcgcca tgctgttcgt
     3481 ggtcgccgtc ggtctcggcc cgttcgctgg cgtgctggcg ctatttatcc acaccaccgg
     3541 cgtgctctcc aagctgcttt ccgaagcggt agaagcgatc gaacccggcc cggtagaagg
     3601 cattcgcgcc accggcgcca acaagctgga agagatcctt tacggcgtgc tgccacaggt
     3661 aatgccgctg ctgatctcct actccctcta tcgcttcgaa tccaacgtcc gctcggcgac
     3721 cgtcgtcggc atggtcggtg caggcgggat cggcgtcacc ctgtgggaag cgattcgcgg
     3781 tttccagttc caacaaacct gcgccctgat ggtgcttatc atcgtcacgg tcagcctgct
     3841 ggatttcctc tctcaacggt tgcgtaagca ctttatctga taagcgaggc attgatatct
     3901 atgcacttgt ctacacatcc gaccagctac ccaacacgct atcaggagat agccgcaaaa
     3961 cttgagcagg agcttcgtca acactaccgc tgcggcgact atcttcccgc cgagcagcaa
     4021 ctggcggcgc gctttgaggt gaaccgccac accctgcgcc gcgccatcga ccaactggtg
     4081 gaaaaaggct gggtacagcg ccgtcagggc gtcggcgtgc tggtgctgat gcgcccgttc
     4141 gattacccgc tcaacgccca ggcgcgtttt agccagaacc tgctggacca gggtagccat
     4201 cccaccagcg aaaaactgct gccggtattg cgccccgcgt ccggtcacgt cgctgacgcg
     4261 ctggggatta ccgagggcga gaacgttatc catttgcgca ccctgcgtcg cgtcaacggc
     4321 gtcgcgctct gtttaatcga ccactacttt gcggacctca ccctttggcc gacgctgcaa
     4381 cgcttcgaca gcggttcgct gcacgatttt ctgcgcgagc aaaccggaat tgcgctgcgc
     4441 cgcagccaga cgcggatcag cgcccgccgc gcccaggcca aagagtgcca gcgccttgaa
     4501 atcccgaata tgtcgccgct gctgtgcgtg cgcaccctta accaccgtga cggtgaaagc
     4561 agcccggcgg agtactccgt cagcctgacg cgcgccgaca tgattgaatt cactatggag
     4621 cactgaatgc acgcagatac cgcgacccgc cagcactgga tgtccgtact ggcgcacagc
     4681 caaccggctg aactggcagc ccgcctgaac gcgctgaaca tcaccgccga ctatgaagtg
     4741 atccgcactg ccgaaactgg cctggtgcag attcaggcac gaatgggcgg taccggcgaa
     4801 cgcttttttg ccggcgacgc cacgctgacc cgcgccgccg tacgcctgac tgacggcacc
     4861 ctcggctaca gctgggtgct ggggcgtgat aaacagcacg ccgaacgctg cgcgctgatt
     4921 gatgcgctga tgcagcaatc tcgccacttt caaaacttat cagaaaccct tattgccccg
     4981 ctggacgctg accgtatggc acgcattgcc gcacgccagg ccgaagtgaa cgccagccgg
     5041 gtcgacttct ttacgatggt tcgcggagac aacgcatgac cctggaaacc gcttttatgc
     5101 ttcccgtgca ggatgcccag cacagttttc gtcgcctgtt aaaggccatg agcgagccgg
     5161 gcgtgattgt cgccctgcat cagctcaagc gcggctggca gccgctgaat atcgccacca
     5221 ccagcgtgct gctgacgctg gccgataacg acacgccggt gtggcttgcc gcgccattaa
     5281 gcaacgacat cgtcaaccag agcctgcgtt ttcataccaa cgcgccgctg gtcagccagc
     5341 cggaacaggc gaccttcgcg gtgacggatg aggcgatttc cagcgaacag cttaacgccc
     5401 tgtccaccgg caccgccgtt gcgccggaag cgggtgcgac gctgatttta caggtcgcca
     5461 gcctgagcgg cgggcgcatg ctgcgcctca ccggcgcggg cattgccgaa gaacgaatga
     5521 tcgctccgca gctgccggag tgcattctgc acgaactcac cgagcgcccg cacccgttcc
     5581 cgctcggcat cgacctgatc ctgacctgcg gcgagcgcct gctggctgtt ccgcgaacca
     5641 ctcatgtgga ggtgtgctga tgtacgttgc cgtgaaaggg ggcgaaaagg cgatcgacgc
     5701 cgcccacgcc ctgcaagaga gccgacgccg gggcgatacc gatttgcctg aactgagcgt
     5761 cgcccagatt gaacagcagc ttaacctcgc agtagatcgc gtgatgaccg aaggcggcat
     5821 tgccgaccgc gaactggcgg cgctggcgct gaaacaggcc agcggcgata acgtcgaagc
     5881 aattttcctg ctgcgcgcct accgcaccac gctggcgaag ctcgcggtaa gcgagccgct
     5941 cgacaccacg gagatgcgcc tcgaacggcg tatttcggcg gtttataaag acattcccgg
     6001 cggccagttg cttggcccta cctacgacta cacccatcgc ctgctcgatt tcaccctgtt
     6061 ggcaaacggt gaagcaccaa cgctgaccac cgccgacagc gaacaacagc cgtcgccgca
     6121 cgttttcagc ctgctggcgc gtcaggggct ggcgaagttt gaagaggata gcggcgcaca
     6181 gccggatgac atcacccgca cgccgccggt ttacccctgc tcacgttctt cccgtttgca
     6241 gcagttgatg cgcggcgacg aaggctattt gctggcgctg gcctactcca cccaacgcgg
     6301 ttacgggcgc aaccacccgt tcgcgggcga gatccgcagc ggctatatcg acgtatcgat
     6361 tgtgccggaa gagctgggat ttgcggtaaa cgtcggcgaa ctgctgatga ccgagtgtga
     6421 aatggttaac ggttttatcg acccgccgga tgaaccgccg tacttcacgc gcggctacgg
     6481 gctggtgttc ggcatgagcg agcgcaaagc gatggcgatg gcgctggttg accgcgctct
     6541 gcaagccccg gagtacggcg agcacgcgac aggcccggcg caggatgaag agttcgtgct
     6601 ggcacatgcc gacaatgtcg aagccgcagg ctttgtctcg cacctcaaac tcccgcacta
     6661 cgtcgatttc caggccgaac tggagctact caaacgtctg caacaggagc agaaccatgg
     6721 ctaatctgag cggctacaac tttgcctacc tcgacgagca gaccaaacgc atgatccgcc
     6781 gcgccatctt aaaagcggtg gcgatccccg gttatcaggt gccgtttggc gggcgcgaga
     6841 tgcccatgcc atacggctgg ggaaccggcg gcatacagct caccgccagc gtgattggcg
     6901 aaagcgacgt gttgaaggtg attgaccagg gcgcggatga caccaccaac gccgtgtcga
     6961 ttcgcaactt ctttaagcgc gtgaccgggg tgaacaccac tgaacgtacg gatgatgcga
     7021 cgcttatcca gacgcgccac cgcattcccg aaacgccgct gacggaagat cagatcatta
     7081 tcttccaggt gccgatcccc gaaccgctgc gctttatcga gccgcgcgaa acggaaaccc
     7141 gcaccatgca cgcgctggaa gagtacggcg tgatgcaggt gaaactgtat gaagatattg
     7201 cccgcttcgg tcatatcgcc accacctacg cctatccggt gaaggtaaat gggcgctacg
     7261 tgatggaccc gtcgccgatc ccgaaattcg ataacccaaa aatggacatg atgcccgccc
     7321 tgcaactgtt cggcgcgggg cgcgagaagc gcatctatgc ggtgccgccg tttacccgcg
     7381 tggaaagtct cgatttcgac gatcacctgt tcaccgttca gcagtgggat gagccatgcg
     7441 ccatctgcgg atcgacccac agctatcttg atgaagtggt gctggatgac gccggaaacc
     7501 gcatgtttgt ctgctccgat accgattatt gccgccaaca gagcgaggca aaaaaccaat
     7561 gaatcaaccg ttactttcgg tcaataacct gacccacctt tacgcgccgg gcaaaggctt
     7621 tagcgatgtc tcttttgatt tatggccggg ggaagtgctg ggcattgtcg gggagtccgg
     7681 ctccgggaag accacgctgc tgaaatcaat ctccgctcgc ctgacgccgc agcaggggga
     7741 gattcactat gagagccgtt cgctgtacgc aatgagcgag gccgaccgcc gtcgcctgct
     7801 gcgcaccgaa tggggcgtag tgcatcagca tccgctcgac ggcctgcgcc gccaagtgtc
     7861 ggcaggcggc aatatcggcg agcggctgat ggcgaccggg gcacgtcatt acggcgatat
     7921 tcgtgccacc gcgcagaagt ggctggaaga ggtggaaatt cccgccaacc ggatcgacga
     7981 cctgccgacc accttttccg gcggcatgca gcaacgtttg cagatcgccc gcaacctggt
     8041 gacgcatccg aagctggtgt ttatggacga gccgaccggc ggactggatg tgtcggtaca
     8101 ggcacgcctg ctcgacctgc tgcgcggcct ggtggtggag ctgaacctcg cggtagtgat
     8161 tgtcacccac gatttaggcg tcgcccgcct gctggcggac cgtttgctgg tgatgaagca
     8221 ggggcaagtg gtggagagtg ggttaaccga ccgcgtgctc gacgacccgc accatccgta
     8281 tacacaactg ctggtgtcat cggttttgca gaattgaatg caacaccgtg cgcgctgatc
     8341 ccctcacccc ggccctctcc cggagggaga gggagaaaac cggcgcaata ttcaacaccg
     8401 cgcacaatcg gtcccctcgc cccttcgggg agagggttag ggtgaggggc aatgcgcgca
     8461 ccgctcccca atatgcgaac cacgaggcga aaaatgatta acgtacaaaa cgtcagtaaa
     8521 accttcatcc tgcaccagca aaacggcgtg cgcctgcccg tcttacaacg cgcctcgctc
     8581 accgtcaacg caggtgaatg cgtggtgctc cacggccatt ccggcagcgg caaatcaact
     8641 ctgctacgct cgctgtacgc caactatctg cccgacgaag ggcaaatcca gatcaaacac
     8701 ggtgacgaat gggtagacct ggtcaccgcg ccagcgcgca aagtggtgga gatccgcaaa
     8761 actaccatcg gctgggtgag ccagtttctg cgcgtcatcc cgcgtatctc agcactggaa
     8821 gtggtgatgc agccgctgct cgatacaggc gttccgcgtg aagcctgcgc cgctaaagcc
     8881 gcgcgtcttc tcacccgcct gaacgtgccg gaacgcctgt ggcacctggc accatcgaca
     8941 ttttccggtg gcgaacagca gcgcgtcaac atcgcccgcg gctttatcgt cgactacccc
     9001 attctgctgc tcgacgaacc taccgcctcg ctggacgcca aaaacagcgc cgcggtggtg
     9061 gaactgattc acgaagccaa agcccgtggc gcagccatcg tcggcatctt ccatgacgaa
     9121 gctgtacgta atgacgtcgc cgaccgcctg cacccaatgg gagcctcttc atgattatca
     9181 ataacgttaa gctggtgctg gaaaacgagg tggtgcacgg ttcgctggag gtgcaggatg
     9241 gcgaaatccg cgcctttgcc gaaagccaga gccgcctgcc agaagcgatg gacggcgaag
     9301 gtggctggct actacccggc ctgattgagc tgcataccga caacctcgat aaattcttca
     9361 ccccgcgccc gaaagtcgac tggcccgccc attcggcgat gagcagccac gacgcgctga
     9421 tggtggcaag cggcatcacc accgtgctgg acgcggtggc gattggcgac gtgcgcgacg
     9481 gcggcgatcg gctggagaat ctggagaaga tgatcaacac catcgaagag acgcagaaac
     9541 gcggcgtcaa ccgcgccgag caccgtctgc acctgcgctg cgagctgccg catcacacca
     9601 cactgccgct gtttgaaaaa ctggttcagc gcgaaccggt gacgctggtg tcgctgatgg
     9661 accactcacc gggccagcgc cagttcgcta accgcgagaa gtatcgcgaa tattatcagg
     9721 gcaaatactc cctcaccgat gcgcagatgc agcagtacga agaagagcaa ttggcgctcg
     9781 ccgcacgctg gtcgcagccg aatcgcgaat ccatcgccgc cctgtgccgc gcgcgacaaa
     9841 ttgcgctcgc cagccacgat gacgccacgc acgcccacgt tgccgaatcc caccaacttg
     9901 gcagcgtgat cgccgaattt cccaccacgt tcgaagcggc ggaagcctcg cgtaagcatg
     9961 gcatgaacgt gctgatgggt gcgccaaata tcgtgcgtgg cggctcgcac tccggcaatg
    10021 tggcggccag tgaactggcg cagcttggtc tgctggatat cctctcttcc gactactacc
    10081 ccgccagcct gctggatgcg gcgttccgcg tcgccgatga cgagagcaac cgctttacgc
    10141 taccgcaggc ggtgaggctg gtgaccaaaa ttccggcgca ggcgctgaat cttcaggatc
    10201 gcggggtgat tggcgagggt aaacgcgctg acctggtgct ggcgcatcgc cagggcaatc
    10261 acattcatat cgaccacgtc tggcgtcagg gtaaaagggt gttctgatga tgggaaaact
    10321 gatttggtta atggggccgt ccggctccgg gaaagacagc ctgctggcgg aactccgcct
    10381 gcgggaacaa acgcagttac tggtggcgca tcgctacatc acgcgcgacg ccagcgccgg
    10441 aagtgaaaac catatcgccc tgagtgagcg ggagtttttt acccgcgcgg ggcaaaacct
    10501 gctggcctta agctggcacg ctaacggtct gtattatggc gtcggcatcg agattgacct
    10561 ctggctgcac gctggattcg acgtagtcgt caacggttca cgcgcgcatt tgccgcaggc
    10621 acgggcgcgc tatcaatcgg cgctgctgcc cgtctgttta caggtttcgc cggagatcct
    10681 gcgccagcgc ctggaaaacc gtggccgtga aaacgccagt gaaattaacg cccgcctggc
    10741 gcgcgccgcc cgctatactc cacaggattg ccatacgctc aacaatgacg gcagcctgcg
    10801 ccagtcggtc gacacgctgc tgacgctgat ccatcagaag gagaaacacc atgcctgctt
    10861 gtgagcttcg cccggccacg cagtacgata ccgacgcggt ttacgcgctg atttgtgagc
    10921 taaaacaggc ggagtttgac caccatgcgt ttcgcgtggg ctttaacgcc aacctgcgcg
    10981 acccaaacat gcgctaccat ctggcgctgc tggatggcga agttgtcggc atgatcggcc
    11041 tgcatttgca gtttcatctg catcatgtca actggatcgg cgaaattcag gaactggtgg
    11101 tgatgccgca ggcgcgcggt ctgaacgtcg gcagtaaatt actggcatgg gcagaagaag
    11161 aagcccgcca ggccggggcc gaaatgaccg agctttcgac caacgtgaag cgccacgacg
    11221 cgcaccgttt ctatctgcga gaaggctacg agcagagtca cttccgcttc accaaggcgc
    11281 tgtaacatga gtctgaccct cacgctcacc ggcaccggcg gcgcacaggg cgttccggca
    11341 tggggctgcg agtgcgcggc ctgcgaccga gcgcggcgct cgccgcagta tcgccgccaa
    11401 ccgtgcagcg gcgtagtgaa gtttaacgag gcgatcaccc tgatcgacgc cgggctgcac
    11461 gatctcaccg atcgctggtc tcccggatcg ttccagcagt ttttgctgac gcattatcat
    11521 atggatcacg tccaggggct gtttccgctg cgctggggcg tgggcgatcc gatcccgatc
    11581 tacggcccgc cggatgaaca gggctgcgac gatctgttta aatatccggg gctgcttgat
    11641 ttcagccaca cggtggaacc gtttgtggtg tttgatttgc aggggttgca ggtcacgccg
    11701 ctgccgctta accactcaaa actcactttc ggctatttac tggaaacggc gcacagccgg
    11761 gtggcgtggc tgtctgacac cgctggcttg ccggaaaaaa cgctgaaatt tttacgcaat
    11821 aatcagccgc aggtaatggt gatggattgc agtcacccgc cgcgcgcgga tgcaccgcgt
    11881 aatcactgtg atttaaatac cgtgcttgcg ctgaatcagg ttatccgctc gccacgggtg
    11941 attctgaccc atatcagcca ccagtttgat gcgtggctga tggaaaacgc actaccgtca
    12001 gggtttgagg tggggtttga tgggatggag attggggtgg cgtgatgaga gggaatgtgc
    12061 gcgctggccc cctcacccta accctctccc cagaggggcg aggggaccga ttgtgctcga
    12121 tattgaatat tgcgctcgtt ttctccctct ccccattggg gtgaggggcg atgcctgctc
    12181 catacccaac ctcatcgccc atactcatct tccattctcc gctcttcatc ttctagctgc
    12241 cgacgctcct gatcaagctg gcgctggcga tcgtccagct gcctgcggcg atcttcaaac
    12301 tggcggcggc ggtcgtcata ttgtctgcgc cgatcgtcgc tcacttcacg ctgccagccg
    12361 tcgtcgcgcg aatcttcata gtctcgccca cggtcagggt tataagcgtc gttaatcgcc
    12421 tgctgaatat tgccaatggt gtcgtcgata atatcggcct gggccggaac ggtggacagc
    12481 gtgagcaggg tgaataaaag aaatagcgga aagcgtttca ttagccaacc tcaaaaagaa
    12541 actctatcca cattaatcat tactcatcca tgcaagtagt ggatgaatct caatttctcc
    12601 gctgctctat tgccgtaatc gcctccacgc gttgttgatg acgaccgcct tcgtactgtg
    12661 cgcccagcca cgcatccaca atcatttttg ccagttcgag gccaaccact cgtgaaccaa
    12721 aagccagtgc aacttcactg gcataatgcg gataatcagt gcgctctgac gacctggtgt
    12781 ctttatcaat cacctcactc attgaccatg aataaaaggc tggcacagca acgcagaatc
    12841 ccgcggtgcc agcgacatga ccgcccattg ctccggtacg tttttataaa gaaacaccgg
    12901 ttgcattgat aaattgtgct cagtatttgc cgaagtacat tatatataat aaatatataa
    12961 aacacctcat attatttaca gagataataa attcgcgtaa ttttccccct tcagcacaaa
    13021 tgcctaataa aatggatcgt tccgctcaca cttccactta ttaaagtcgc aaattcccaa
    13081 ttatgataac cccagcttta aatgacgtat tttgcatgga ttacatattt acgacataaa
    13141 tgataaatgg agatttatat gaataactct cggttattcc gtttgagcag aattgttatt
    13201 gcgttaactg ccgccagcgg catgatggta aataccgcta acgtgaaaga ggaagcgaaa
    13261 gccgccactc aatataccca acaggttaat cagaattacg ccaaatcatt accgtttagc
    13321 gatcgtcagg attttgacga tgcccagcgt ggatttatcg ctccgctgct ggatgaaggt
    13381 attctgcgcg atgctaatgg caaaccatat tatcgcgggg aagattataa atttgatatc
    13441 aatgcgcccg cgcccgaaac cgttaaccct agcctgtggc gtcagtcgca gttaaacggt
    13501 atttccggcc tgtttaaagt caccgacaga atgtaccagg ttcgcggtca ggatatctcc
    13561 aacatcacct ttatcgaagg tgataccggg attattgtca tcgacccgct ggtgacgcca
    13621 cctgccgcaa aagccgccct tgacctttac ttccaaaatc gcccgcaaaa accgattgtt
    13681 gcggttattt atacccacag ccacaccgac cactatggcg gtgtgaaagg cattatctct
    13741 gaagccgatg ttaaatcagg caaagttcag gtgattgccc cagcaggctt tatggacgaa
    13801 gccatcagcg aaaacgtgct ggcggggaac atcatgagcc gccgtgcgct ctactcttac
    13861 ggtctgttac tgccgcacaa cgcgcaaggc aacgtgggta atggccttgg cgtgacgctg
    13921 gcaacgggcg acccgagcat catcgcaccg aacaaaacca tcaccaaaac cggtgagaag
    13981 atgattatcg acggcctgga gtttgacttc ctgatgaccc caggtagcga agcaccagcc
    14041 gaaatgcact tctatattcc ggccctgaaa gcgctgtgta ccgccgagaa cgccacgcat
    14101 accctgcaca acttctacac tctacgcggc gcgaaaaccc gcgacaccag caagtggacc
    14161 gagtatctga acgaaacgct ggatatgtgg ggtaacgacg cggaagtcct gtttatgccg
    14221 cacacctggc cggtctgggg caataagcat atcaatgatt atattggtaa atatcgcgat
    14281 actatcaagt acattcacga ccagaccctg cacctggcga accagggcta caccatgaat
    14341 gaaatcggcg acatgattaa actgccgcct gcacttgcca ataactgggc cagccgtggc
    14401 tattacggtt ctgtcagcca caacgcccgc gcggtgtata acttctatct tggctattac
    14461 gacggtaacc cggctaacct gcatccgtat ggtcaggtgg agatgggtaa acgttacgtg
    14521 caggcgctgg gcggttccgc acgcgttatt aacctggcgc aagaagcgaa caagcaaggt
    14581 gattaccgct ggtcggcaga actgctgaaa caggtgattg ccgccaaccc gggtgaccag
    14641 gtcgcgaaga atctgcaagc gaataacttt gaacagctgg gctatcaggc cgagtccgcc
    14701 acctggcgcg gtttctacct gaccggcgcg aaggagctgc gcgaaggggt gcataagttc
    14761 agccacggca ccaccggttc cccggacacc attcgcggga tgtcggtcga aatgctgttc
    14821 gactttatgt ccgttcgcct cgatagcgcg aaagccgcgg gtaaaaatat cagcctgaac
    14881 ttcaatatga gcaatggcga taacctcaac ctgacgctga acgatagcgt gcttaactac
    14941 cgtaaaacgc tgcaaccgca agctgacgcc tctttctaca tcagccgtga agatctgcac
    15001 gccgtgctga ccggacaagc caaaatggcg gatctggtaa aagcgaagaa agccaaaatt
    15061 attggcaatg gcgcgaaact ggaagaaatt atcgcctgtc tggataattt cgatttgtgg
    15121 gtgaatatcg taaccccaaa ttaatgtcac tcgcttttgg gggc
//
LOCUS       DAZKHC020000095        15097 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZKHC020000095 DAZKHC020000000
VERSION     DAZKHC020000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.095"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            253..3351
                     /gene="ygfK"
                     /locus_tag="ACHOSM_RS18345"
                     /old_locus_tag="pgaptmp_003669"
     CDS             253..3351
                     /gene="ygfK"
                     /locus_tag="ACHOSM_RS18345"
                     /old_locus_tag="pgaptmp_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HGU4560096.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDICGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLLVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSKGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            3354..4682
                     /gene="ssnA"
                     /locus_tag="ACHOSM_RS18350"
                     /old_locus_tag="pgaptmp_003670"
     CDS             3354..4682
                     /gene="ssnA"
                     /locus_tag="ACHOSM_RS18350"
                     /old_locus_tag="pgaptmp_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HGU4560097.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SFKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            4733..5512
                     /gene="ygfM"
                     /locus_tag="ACHOSM_RS18355"
                     /old_locus_tag="pgaptmp_003671"
     CDS             4733..5512
                     /gene="ygfM"
                     /locus_tag="ACHOSM_RS18355"
                     /old_locus_tag="pgaptmp_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HGU4560098.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            5509..8379
                     /gene="xdhD"
                     /locus_tag="ACHOSM_RS18360"
                     /old_locus_tag="pgaptmp_003672"
     CDS             5509..8379
                     /gene="xdhD"
                     /locus_tag="ACHOSM_RS18360"
                     /old_locus_tag="pgaptmp_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HGU4560099.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIEVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            8544..9944
                     /gene="xanQ"
                     /locus_tag="ACHOSM_RS18365"
                     /old_locus_tag="pgaptmp_003673"
     CDS             8544..9944
                     /gene="xanQ"
                     /locus_tag="ACHOSM_RS18365"
                     /old_locus_tag="pgaptmp_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HGU4560100.1"
                     /translation="MSDINNAGSDLIFELEDPPSFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            9962..11278
                     /gene="guaD"
                     /locus_tag="ACHOSM_RS18370"
                     /old_locus_tag="pgaptmp_003674"
     CDS             9962..11278
                     /gene="guaD"
                     /locus_tag="ACHOSM_RS18370"
                     /old_locus_tag="pgaptmp_003674"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HGU4560101.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            11314..12681
                     /gene="ghxQ"
                     /locus_tag="ACHOSM_RS18375"
                     /old_locus_tag="pgaptmp_003675"
                     /gene_synonym="ygfQ"
     CDS             11314..12681
                     /gene="ghxQ"
                     /locus_tag="ACHOSM_RS18375"
                     /old_locus_tag="pgaptmp_003675"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HGU4560102.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVTFYA
                     GGWAI"
     gene            complement(12717..13205)
                     /gene="ygfS"
                     /locus_tag="ACHOSM_RS18380"
                     /old_locus_tag="pgaptmp_003676"
     CDS             complement(12717..13205)
                     /gene="ygfS"
                     /locus_tag="ACHOSM_RS18380"
                     /old_locus_tag="pgaptmp_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU4560103.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(13205..15067)
                     /gene="ygfT"
                     /locus_tag="ACHOSM_RS18385"
                     /old_locus_tag="pgaptmp_003677"
     CDS             complement(13205..15067)
                     /gene="ygfT"
                     /locus_tag="ACHOSM_RS18385"
                     /old_locus_tag="pgaptmp_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HGU4560104.1"
                     /translation="MACAVAHNQENWPLSHSDFRPRIHVVGKGQAANPVACHHCNNAP
                     CVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVVEMVDTIAQKCDLCNQRSSG
                     TQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASSDAQPSRSAALLPVNSRKGA
                     DKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKANCNWHCPLHNAIPDYIRLVQ
                     EGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKDHSGAVSIGNLERYITDTAL
                     AMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGVQVDVFDRHPEIGGMLTFGI
                     PPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDLTSEYDAVFIGVGTYGMMRA
                     DLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVEGKRVVVLGGGDTTMDCLRT
                     SIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQFNVQPQYIACDEDGRLTAVG
                     LIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQAHAMPWLQGSGIKLDKWGL
                     IQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGRQAARDMLTLFDTKAS"
BASE COUNT         3592 a         3936 c         3888 g         3681 t
ORIGIN      
        1 tcactgattt tgagaaaaga gaaaaattga taaatggact ggcaataata attttcagga
       61 gtgattttaa ttctcatttg gcacctgtga gttcattcac aaaaagccct taagtttcga
      121 gccgcctcac atttttttat atttcccgcc aaacctggca agagtggtgc gattgttgct
      181 ctatccccct aaaccaccgg atttctcaac accggtcact caatgatatc tgtataagct
      241 aaggagaggg ttatggggga tattatgcgt cccattccgt ttgaggaact tttgacgcgc
      301 atatttgatg aataccaaca acaacgctca atctttggta ttcccgagca acagttttac
      361 tcacccgtaa aaggtaaaac tgttagcgtc ttcggtgaaa cctgtgccac tcccgtcggc
      421 cctgccgctg gcccgcacac gcagctcgcg caaaatattg tcacttcctg gctgactggc
      481 ggacgcttca tcgaactaaa aaccgtccaa attcttgacc gcctggagct ggaaaagccc
      541 tgtatcgatg ccgaagacga gtgctttaac accgaatggt ctaccgaatt taccctgctt
      601 aaagcctggg atgaatacct caaagcctgg tttgccctgc accttctcga agcgatgttc
      661 cagccttctg attccggtaa atcgttcatc tttaatatga gcgtcggtta caacctcgaa
      721 ggtattaagc aaccgccaat gcaacagttc atcgacaata tgatggacgc atctgaccat
      781 ccgaaattcg cgcaatatcg cgatacgctg aataaattac tccaggatga cgcattttta
      841 gcccgccacg gattgcagga aaaacgcgaa agcttgcaag ccttacccgc tcgcatcccc
      901 accagtatgg tgcagggcgt caccctctcc accatgcacg gctgtcctcc gcatgaaatc
      961 gaagccattt gccgctacat gctggaagaa aaagggctca acacctttgt gaaactcaac
     1021 ccgacattac tggggtacgc gcgtgttcgt gagatcctcg atatctgcgg tttcggttac
     1081 ataggcttaa aagaagagtc atttgatcac gacctcaagc tgacgcaagc gctggaaatg
     1141 ctggaacgcc tgatggcact ggcaaaagaa aaatcactcg gctttggcgt aaaactgact
     1201 aacactctcg gcaccatcaa caataaaggc gcactgcctg gtgaagagat gtatatgtca
     1261 ggccgtgcgc tgttcccgct ctccatcaat gttgcagcag ttctctctcg cgcctttgac
     1321 ggcaaactgc ccatttctta ttccggtggt gccagtcagc tgactatccg cgatattttt
     1381 gatactggta ttcgccctat tactatggca accgatctgc tgaaacctgg cggctatctg
     1441 cgcttaagtg cctgcatgcg cgagctggaa ggctccgacg cctggggact tgaccatgtt
     1501 gacgtcgaac gactgaaccg actggcagca gacgcgttaa ccatggaata cacccagaaa
     1561 cactggaagc cagaagagcg tattgaagtg gcagaagacc tgccgctgac cgattgctac
     1621 gttgccccct gtgttactgc ctgcgctatc aagcaagata ttccggaata catccgtctg
     1681 cttggcgaac accgctatgc cgacgcgctg gaactcatct accaacgcaa cgccctacct
     1741 gccattaccg gtcatatttg cgatcaccag tgccaataca actgtacccg cctggattac
     1801 gacagtgcgc tgaatatccg cgaactgaaa aaagtcgcgc tggaaaaagg ttgggatgaa
     1861 tataagcaac gctggcacaa acccgccggt tctggttcac gccatccggt tgccgtgatt
     1921 ggtgcaggtc cggcgggtct ggcagcaggt tacttccttg ccagagcggg ccatccggtt
     1981 acgctgtttg aacgcgaagc caatgctggc ggcgtggtga aaaatatcat tcctcagttc
     2041 cgtattcctg cagagttaat tcagcacgat atcgattttg ttgccgctca cggcgtgaaa
     2101 tttgagtatg gctgctcacc cgatttaacc gttgaacagt taaaaaatca gggcttccac
     2161 tatgttctga ttgccaccgg cactgataaa aatagcggtg tgaaactggc gggcgacaac
     2221 caaaatgtct ggaaatcact ccccttcctg cgtgaataca acaagggtac agcgctcaag
     2281 ctgggcaaac atgtggtcgt tgtcggggcg ggtaacaccg caatggactg cgctcgtgcg
     2341 gcgttacgcg ttccaggcgt agaaaaagca acgatcgttt accgtcgttc actacaagag
     2401 atgcccgcat ggcgcgaaga gtatgaagaa gcgttgcacg acggcgtaga gttccgtttc
     2461 ctgaataatc cggaacgttt cgatgctgat ggcaccttaa ccttgcgcgt tatgtcgctt
     2521 ggcgaaccgg atgagaaagg tcgtcgtcgt ccggttgaaa ccaatgaaac agtaacactg
     2581 cttgtagaca gcctgatcac cgccattggt gaacagcagg atactgaagc cctgaatgcg
     2641 atgggcgtgc cgctggacaa aaacggctgg ccagacgtcg accataatgg cgaaactcgt
     2701 ctgactgacg tctttatgat cggcgacgta cagcgcggac catcctccat tgtcgctgct
     2761 gtcggaaccg cgcgtcgggc gaccgatgcc atccttagtc gggaaaatat ccgttcccac
     2821 cagaacgata aatactggaa caacgtcaat ccagcggaaa tctatcaacg taaaggcgat
     2881 atctctatca cgctggtgaa cagtgacgat cgtgacgcgt ttgtcgccca agaagccgct
     2941 cgctgcctcg aatgtaacta cgtttgcagc aagtgtgtgg atgtctgccc gaaccgcgcc
     3001 aacgtctcca ttgcggtccc aggcttccag aaccgtttcc agacgctgca cctcgacgct
     3061 tactgtaacg aatgcggcaa ctgcgctcag ttctgtccgt ggaacggtaa accgtacaaa
     3121 gacaaaatca ccgtcttcag cctggcgcaa gactttgata acagcagcaa cccaggcttc
     3181 cttgtggaag attgccgggt acgagtacgt ctgaataacc aaagctgggt gttaaacatc
     3241 gacagcaaag gtcagtttaa caacgtacca ccggagctga acgatatgtg ccgcatcatc
     3301 agccatgtcc accagcatca tcattatctg ctgggccgcg tggaggtgta atcatgttga
     3361 ttctgaagaa tgtcactgcg gtacagctac acccggcaaa agtgcaggaa ggcgttgata
     3421 tcgccatcga aaacgatgtg attgtcgcta tcggcgatgc cctgacgcaa cgctaccccg
     3481 acgccagctt caaagagatg catggccgga ttgtgatgcc agggattgtc tgctcgcaca
     3541 accattttta ctcggggctt tcccgcggaa ttatggcaaa catcgcccct tgcccggatt
     3601 tcatctcaac gctgaaaaat ctctggtggc ggctcgatcg cgcccttgat gaagagtcgc
     3661 tctattacag cggactgatt tgttccctgg aagcgattaa gagcggatgt acatcggtta
     3721 tcgatcacca tgcctctccg gcgtatatcg gcgggtcgct ctccacattg cgcgacgcat
     3781 ttttaaaagt tggcctgcgc gcgatgacct gttttgaaac tactgaccgt aacaacggca
     3841 tcaaagagtt gcaggaaggt gtagaagaaa acatccgttt cgcccgtttg attgatgagg
     3901 cgaagaaagc gacaagcgag ccgtatctgg tggaagcaca tatcggtgct cacgcgccgt
     3961 ttaccgtgcc ggatgccggt ctggagatgc tgcgtgaagc cgtgaaagcc acaggccgtg
     4021 gtttgcatat tcacgctgcg gaagaccttt acgacgtttc ctacagtcac cactggtacg
     4081 gcaaagacct gctggcacga ctggcgcaat tcgacctcat cgacagcaaa acgctggtcg
     4141 ctcatgggct gtacttgtcg aaagatgaca tcgccctact caatcagcgc gatgcgttcc
     4201 tggtgcataa cgcccgttca aacatgaaca accatgtcgg ctacaaccat caccttagcg
     4261 acatccgcaa cctggcattg ggaacggacg gcattggttc ggacatgttt gaagagatga
     4321 aatttgcctt ctttaaacat cgcgatgcgg gtggtccgct gtggcctgac agttttgcca
     4381 aagccctgac taacggtaac gaactgatga gccgcaactt tggcgcgaaa tttgggcttc
     4441 tggaagccgg ttacaaagct gatttgacca tttgcgatta caactcgccg acgccgctgc
     4501 tggcagacaa tatcgccggg catatcgctt tcggtatggg ctcaggcagc gttcacagcg
     4561 tgatggtcaa tggtgtgatg gtctatgaag accgtcagtt taacttcgat tgcgattcca
     4621 tttatgcaca agccagaaaa gccgctgcca gtatgtggcg tcggatggat gcgctggcat
     4681 aaatgacaga tgccctcttc ccgcagggaa gagggttaaa taaggaaaga agatgattga
     4741 acaatttttc aggcccgact ctgtcgaaca ggcgctggaa ctgaagcgcc gctaccagga
     4801 tgaagccgtc tggttcgccg ggggcagcaa actcaatgct acaccaaccc gtaccgataa
     4861 aaagattgcc atttccttac aggatctgga actggactgg gttgactggg ataacggtgc
     4921 actgcggatt ggcgcaatgt ctcgcttgca gccactgcgt gatgcgcgat ttattcctgc
     4981 agcgctgcgt gaagccctcg gttttgttta ctcacgccat gttcgtaatc agtcgaccat
     5041 tggtggtgaa atcgccgccc gccaggaaga gtcggtgctg cttcccgtcc tgttggcact
     5101 ggatgctgaa ctggtttttg gcaacggcga aacgctgtca atcgaggact acctggcctg
     5161 cccatgcgat cgcctgctaa ccgaaattat cattaaagat ccgtatcgca cctgtgcgac
     5221 ccgcaaaatt agccgttctc aggcaggttt aaccgtcgtg acggcagccg ttgcaatgac
     5281 agaccacgac ggtatgcgaa ttgcgctgga tggcgtcgcc agtaaagcac tgcgtctgca
     5341 tgatgtcgaa aaacaaaatc tggaaggcaa tgcacttgaa caggctgtcg ccaacgccat
     5401 tttcccgcag gaagatttgc ggggcagcgt ggcctataaa cgctatatca cgggagttct
     5461 ggtagccgac ctgtatgccg actgccaaca ggctggggag gaagccgtat gatcatccac
     5521 tttactttaa atggcgcgcc tcaggagcta accgttaatc caggcgaaaa cgtgcaaaag
     5581 ctgttgttta acatgggaat gcactctgta cgcaacagtg atgatggttt cgggtttgcc
     5641 ggttctgacg caatcatctt taacggtaat atcgttaacg cgtccttgct tattgccgca
     5701 cagttagaga aggcagatat tcgtaccgca gaatctctgg gtaaatggaa cgagttaagt
     5761 ctggttcaac aggcaatggt tgatgttggc gtggtgcagt ctggttataa cgatccagct
     5821 gcagctctga ttatcaccga tcttctcgat cgcatcgccg cacctacccg cgaagagatc
     5881 gacgacgcgc tttctggttt gttcagccgc gatgcaggct ggcaacaata ctatcaggtc
     5941 attgaactgg cggttgcacg taaaaataat ccgcaggcca ccattgatat cgctccgact
     6001 ttccgtgacg accttgacgt cattggcaag cattatccta aaactgatgc cgcgaaaatg
     6061 gtgcaggcga aaccctgcta tgttgaagac cgcgtaacgg ctgacgcctg cgtcattaaa
     6121 atgttacgta gcccacacgc tcacgcactg attactcatc tggatgtcag caaagctgaa
     6181 gccttaccgg gcgtcgttca cgttattact cacctgaatt gccctgatat ctactatacc
     6241 ccggggggtc agagcgcacc ggaaccgtca ccgcttgacc gccgtatgtt cggcaaaaaa
     6301 atgcgtcacg tcggcgatcg cgttgctgcg gtggtcgctg aaagtgaaga aattgcgctc
     6361 gaagcattga aactcatcga ggttgaatat gaagtgctta agccggtaat gtcgatcgac
     6421 gaagcaatgg cggaagatgc gcctgtcgtg cacgatgaac cggtggtgta tgttgctggt
     6481 gcgccagata ctctggaaga cgataacagc catgcagccc agcgcggcga gcatatgatc
     6541 atcaacttcc cgatcggttc tcgccctcgc aaaaatatcg ccgccagtat tcatggtcat
     6601 attggcgata tggacaaagg ctttgccgat gccgatgtga tcattgagcg aacctataac
     6661 tcaacgcagg cacagcagtg cccgactgaa acacatatct gctttacccg gatggacggc
     6721 gatcgtctgg ttatccacgc ctccacccag gtaccatggc acttacgccg ccaggtcgcg
     6781 cgcctcgtgg gcatgaaaca gcataaagtt catgtcatta aagagcgagt tggcggcggt
     6841 tttggttcca aacaggacat cctgctggaa gaagtgtgcg cctgggcaac ctgcgtgacc
     6901 gggcgtccgg tactgttccg ctacacccgt gaagaagagt ttattgctaa cacctctcgt
     6961 cacgtcgcga aagtcaccgt caaactggga gcgaaaaaag atggtcgcct gacggcagtg
     7021 aagatggatt tccgcgccaa cactggccct tacggcaacc actcactcac cgtaccgtgt
     7081 aacggaccgg cgctgtcgct gccgttatat ccgtgcgata acgtcgattt ccaggtcacc
     7141 acctactaca gcaacatttg cccaaatggt gcttatcagg gttatggcgc accgaaaggt
     7201 aacttcgcta tcaccatggc attagcggaa ctggctgaac agttacagat cgaccaactg
     7261 gaaattatcg aacgtaaccg ggtacacgaa gggcaagagc tgaaaattct cggtgcaatc
     7321 ggtgaaggta aagcgccgac ctccgttcct tccgccgcca gctgtgcact ggaagagatc
     7381 ctgcgtcagg gtcgcgagat gatccaatgg tcgtcaccga aaccgcaaaa tggtgactgg
     7441 cacatcggtc gcggcgtcgc cattatcatg cagaaatcgg gtatcccgga tatcgatcag
     7501 gctaactgca tgatcaaact ggaatcggac ggcaccttta tcgttcattc tggcggtgcg
     7561 gatattggta ctggtctgga tactgtagtg acgaaactgg cagcagaagt gctgcactgc
     7621 ccaccgcagg acgtgcatgt tatctccggt gataccgatc atgcgttgtt tgataaaggc
     7681 gcatatgcct cgtccggtac ttgcttctca ggtaacgcgg cgcgtttggc agcggaaaat
     7741 ctgcgggaga aaattctgtt ccacggcgcg caaatgttgg gtgaggcagt ggcagatgtt
     7801 caactggcaa cgccgggcgt cgtgcgcggc aagaaaggcg aagtcagctt cggggatatt
     7861 gctcataaag gcgaaaccgg caccggcttt ggttcactgg tgggaactgg cagttatatc
     7921 acgcctgatt tcgccttccc gtatggcgca aacttcgctg aagttgccgt caacacgcgt
     7981 acgggtgaaa tccgcctgga taaattctac gccttgctgg actgcggtac accggtcaac
     8041 ccggaattag cattgggaca aatctacggt gctaccctgc gagctatcgg ccacagtatg
     8101 agcgaagaga tcatttatga cgccgaaggt cacccgttaa cgcgtgattt acgcagttac
     8161 ggcgcaccga aaattggtga cattccgcgt gatttccgcg ctgtgctggt gccgagcgac
     8221 gataaagtcg gcccgttcgg ggcgaaatcg atctcggaaa tcggtgtaaa tggcgcagct
     8281 ccggcgattg ctaccgcaat tcacgatgca tgcggcatct ggttacgcga atggcatttc
     8341 acaccggaga aaatactcac tgcgctggaa aaaatataaa taataaatgg ccgcttcggc
     8401 ggcctgtttc tctgtttgga cgatgactaa atagtgactg cgaaaatgag ataagccgtt
     8461 ctcaaggcgt cctgcgcccc aaattcgtgg gaatttattt tactaattca gatgatcaaa
     8521 tttactttaa aaggagtgaa gggatgtctg atataaacaa tgcaggttct gaccttatat
     8581 ttgaactgga ggatccccct tcctttcatc aggctctcgt tggtgccatt acccatctgt
     8641 tggcaatttt cgttccgatg gtaacccccg cgttaatcgt gggggcggcc ttacagcttt
     8701 ccgctgaaac aactgcctat cttgtttcta tggcgatgat cgcctctggt attggtacct
     8761 ggttacaagt aaaccgctac ggcatcgtcg gttctggcct actctcaatt cagtcagtca
     8821 atttttcatt tgttacggtc atgattgcgc tgggcagcag catgaaaagc gacggttttc
     8881 acgaagagtt aatcatgtcg tcgcttctcg gcgtctcctt cgttggcgca tttctggttg
     8941 tcggatcttc atttatcttg ccctatttac gtcgggttat tacgcctacc gtcagcggta
     9001 ttgtggtact gatgatcggc ttaagcctga ttaaagtcgg cattatcgat tttggtggag
     9061 gatttgcagc caaaagcagc ggtacgttcg gcaattacga acatctcggc gttggtttat
     9121 tggttttaat tgtggtgatc ggctttaact gctgtcgcag tccgttgcta cgcatgggag
     9181 ggatcgccat tgggctatgt gtcggctata tcgcatcgtt atgcctgggc atggtggatt
     9241 tcagcagtat gcgcaatttg ccgttaatca ccatcccgca tccgttcaaa tacggcttta
     9301 gttttagctt ccatcagttc ctggtggttg gcacgattta tctgcttagc gtgctggaag
     9361 cagtcggcga tatcaccgcc acggcaatgg tttcccgccg ccccattcag ggggaagagt
     9421 atcagtcccg gctgaaaggc ggcgtgctgg cagatggtct ggtttctgtt atcgcctccg
     9481 ctgtcggttc attaccatta accacgtttg cgcaaaataa tggggttatt cagatgactg
     9541 gcgtcgcttc acgttatgtc gggcgaacca tcgcggtaat gctggttatc ctcggcttat
     9601 ttccgatgat tggcggcttc ttcacgacca ttccctcggc agttctggga ggcgcaatga
     9661 cgttgatgtt ttccatgatt gccatcgcag ggattcgcat catcatcacc aacggtttaa
     9721 agcgccgtga aacacttatt gtcgccactt ctttaggttt agggcttggc gtctcctacg
     9781 atcccgaaat ttttaaaata ttgccagcct ctatttatgt attagttgaa aaccctattt
     9841 gtgctggcgg gttaactgcg attttattaa atattatcct ccctggtggc taccgacagg
     9901 aaaacgttct gcctggtatt acctcagcgg aagagatgga ttaacagtaa aggagtcaat
     9961 gatgtcagga gaacacacgt tgaaagcggt acgaggcagt tttattgatg tcacccgtac
    10021 ggtcgataac ccggaagaga ttgcctctgc gctgcggttt attgaggatg gtttattact
    10081 cattaaacag ggaaaagtgg aatggtttgg cgaatgggaa gacggaaagc atcaaattcc
    10141 tgacactatt cgcgtgcgcg actatcgcgg caaactgata gtaccgggct ttgtcgatac
    10201 acatatccat tatccgcaaa gtgaaatggt gggggcctat ggtgaacaat tgctggagtg
    10261 gttgaataaa cacaccttcc ctactgaacg tcgttatgag gatttagagt acgcccgcga
    10321 aatgtcggca ttcttcatca agcagctttt acgtaacgga accaccacgg cgctggtgtt
    10381 tggcactgtt catccgcaat ctgttgatgc gctgtttgaa gccgccagtc atatcaatat
    10441 gcgtatgatt gccggtaagg tgatgatgga ccgcaacgca ccggattatc tgctcgacac
    10501 tgccgaaagc agctatcacc aaagcaaaga actgattgaa cgctggcaca aaaatggtcg
    10561 tctgctatat gcgattacgc cacgcttcgc cccgacctca tctcctgaac agatggcgat
    10621 ggcgcaacgc ctgaaagaag aatatccgga tacgtgggta catacccatc tctgtgaaaa
    10681 caaagatgaa attgcctggg tgaaatcgct ttatcctgac catgatggtt atctggatgt
    10741 ttaccatcag tacggcctga ccggtaaaaa ctgtgtcttt gctcactgcg tccatctcga
    10801 agaaaaagag tgggatcgtc tcagcgaaac caaatccagc attgctttct gtccgacctc
    10861 caacctttac ctcggcagcg gcttattcaa cttgaaaaaa gcatggcaga agaaagttaa
    10921 agtgggcatg ggaacagata tcggtgccgg aaccactttc aacatgctgc aaacgctgaa
    10981 cgaagcctac aaagtattgc aattacaagg ctatcgcctc tcggcatatg aagcgtttta
    11041 cctggccacg ctcggcggag cgaaatctct gggccttgac gatttgattg gcaacttttt
    11101 acctggcaaa gaggctgatt tcgtggtgat ggaacccacc gccactccgc tacagcagct
    11161 gcgctatgac aactctgttt ctttagtcga caaattgttc gtgatgatga cgttgggcga
    11221 tgaccgttcg atctaccgca cctacgttga tggtcgtctg gtgtacgaac gcaactaata
    11281 ataaaacttt aacatcctcg tgaggacatc attatgtctg gagacatcct acaaacaccg
    11341 gacgcaccaa agccacaggg cgcgctggat aattatttta aaattaccgc tcgtggcagt
    11401 accgttcgtc aggaagtact ggctggctta acgacctttc tggccatggt ttattccgtt
    11461 atcgtcgttc cgggaatgct gggcaaagca ggttttcctc ccgcagctgt gtttgttgcc
    11521 acctgtctgg tcgcgggctt cggctcgttg ctgatggggt tgtgggccaa tttgccaatg
    11581 gcgattggtt gtgcgatttc cttgacggca tttaccgcat tcagtctggt actcgggcaa
    11641 caaattagcg ttcctgtcgc actgggcgcg gtctttctga tgggcgtcat cttcaccgct
    11701 atttccgtaa ccggtgtgcg tacctggatc ttacgtaatt taccgatggg tatcgctcac
    11761 ggtacaggta tcggtatcgg cctgtttctg ctgctgattg ctgctaacgg tgtgggtatg
    11821 gttatcaaaa acccgattga aggcttgcca gtggcgctcg gtgcgtttac ctccttcccg
    11881 gtgatgatga gcttgctggg gctggcggtc atcttcggtc tggagaagtg ccgcgtaccc
    11941 ggcgggatct tgttggtgat tatcgcaatt tcgattatcg gcttaatctt tgacccagcg
    12001 gtgaaatacc acggtctggt ggcgatgcca agtctgactg gcgaagatgg taagtctctg
    12061 attttcagcc tcgatattat gggcgcactc cagccaaccg tacttccgag tgtactggca
    12121 ttggtgatga ccgcagtgtt cgacgccact ggcaccatcc gtgccgtcgc cggtcaggcg
    12181 aatttgttgg ataaagacaa ccagatcatc aatggcggca aagccctgac cagtgactca
    12241 gtaagttcaa tattctccgg tctggtgggc gcagcgcccg cagcggttta tatcgaatca
    12301 gcggcaggaa ccgccgccgg gggtaaaaca gggttgaccg caaccgtagt gggggcgtta
    12361 ttcctgctga ttctgttctt atcaccgctg tcatttttga tccctggtta cgccactgca
    12421 cccgctctga tgtacgtggg tttgctgatg ttaagtaacg tctcgaagct ggatttcaac
    12481 gattttattg acgcaatggc tggcctggtg tgtgccgtgt tcatcgttct gacttgtaat
    12541 atcgttaccg gtattatgct gggctttgtg acactggtcg taggccgtgt ctttgctcgc
    12601 gaatggcaaa agttgaatat tggtacggtg atcattactg ccgcactggt cacattttac
    12661 gcgggtggtt gggcaatcta atagattctc cgcgcctttc agcgcggaga tttctttcac
    12721 agaggattct caccgctggc aacaatacgc tgctggcgga ctcgcctgag ttctctttca
    12781 gtaagcaact gcagcgcctg tgtcgggcaa gattcaacac aggctggccc ctcttcccgc
    12841 tgttcacaga gatcgcattt cacaatttgt tgtcgggtat cccccggcaa cgactgaatg
    12901 gtgatcattc caaacgggca cgcactgacg caactctgac agccaataca acgggcagaa
    12961 ttggtctgca ccacctgctc gcccatcgtc agcgccccca cggggcaagc gccaacacaa
    13021 ggggcgtttt cacactgatg gcacatcacc ggcgcactga tgctgtccag tcgctgtacc
    13081 ttcagccggg gtaaaaagac atcggcattc aactcctgtt ctgaagggtg agcgaccaca
    13141 caggctactt cacaagtacg gcagccaata cagtcagccg gattaacgat aattaacgat
    13201 ttcatcacga tgccttcgta tcaaacagag ttaacatatc gcgcgccgcc tgccttcctg
    13261 cggccattgc agtgacaacc agatccgcgc catgaactgc atcaccacca gcaaagactt
    13321 ttttcagatg cgtctgggta ggtaaatacc cgacgtcacc ggtttgaatc aggccccatt
    13381 tatcgagttt aattccgctg ccctgcaacc acggcatggc atgcgcctgg aaaccaaagg
    13441 ccataatcag aacatcggcg ggcaattcaa actctgaacc cgctaccgga cgaggacgac
    13501 ggcgaccatc cggccccggc tcacccatgg cggtacgaat caggcccacc gcagttaaac
    13561 gcccatcttc gtcacaagcg atatattgcg gttgaacatt gaactgaaac tcgacacctt
    13621 cctcgcgcgc attaaccacc tctttgcgcg agcccggcat actgacttca tcacgacgat
    13681 acgcgcaggt cacgctggcg gcattgaggc ggatggaagt ccgcaaacaa tccattgtcg
    13741 tatcgccacc gcccaatacc acgactcgct taccttccac gtccgtcagc ggatactctt
    13801 cagactccgg caatcccatg agctggcggg tatgggcagt caggaacggt agagcctgaa
    13861 tgacaccggg cgcatcttca tgcggcagat ctgctcgcat catcccgtaa gtccccacgc
    13921 cgatgaaaac tgcatcatat tcagaagtta aatcgctaaa ggtaatatcg cggccaattt
    13981 cacagttaag atgaaagtcg attcccattg cggtgaatat ctctcgccgc tggcttaata
    14041 ccgttttatc gagtttgaaa ggaggaatgc caaaagtcag cataccgcca atttctggat
    14101 ggcgatcaaa gacatcgacc tgaactcctg cgcgcgccag aatatcagca caccctaacc
    14161 ctgcaggccc agcgccaatc accgccactt tttcgctacg gggaacaact ttgctgacat
    14221 cgggacgcca gcccatcgcc agcgcggtat cggtgatgta gcgttccaga ttaccgatag
    14281 agactgcgcc cgagtgatct ttcaaagtac atgcgccttc acaaagacgg tcctgtggac
    14341 ataccctgcc gcaaatttct ggtaaggaac tggtctggtg gcaaagttct gccgcttcaa
    14401 taatctttcc ttcctgtacc agacggatgt aatccggaat agcgttatgc agcgggcaat
    14461 gccagttgca gttagctttt tcggcacaat aaacacagcg gtcactctca taagtcgctt
    14521 gttgtggatc cagcccgcaa tatatttcgc caaagtgggt tttccgttca ctcgctgaaa
    14581 ttttatctgc gcctttacgc gagttaacgg ggagcaacgc tgcactgcga gatggctgag
    14641 cgtctgatga cgcttttcct gctgccgttt tgcgctggcg ggccaccttt atctgctgta
    14701 accctttatc gtccatcagt cgtaacgcct gtgttgggca gacttcaata caggcttgcg
    14761 tgccggaact gcgctggtta caaaggtcgc atttctgcgc aatcgtatcg accatctcaa
    14821 cgacgccaaa ggggcaagcg attgcgcatc ttttacagcc aatacatttt tgctcgtcca
    14881 gttgtacgct atcggactgg aaagtcagag cattaaccgg acaagccgta acgcaagggg
    14941 cattgttgca gtgatggcag gccaccggat tcgcagcctg gcctttccct acaacgtgga
    15001 tacgcggtcg aaagtcactg tgactcagag gccagttttc ttgattgtgt gccaccgcac
    15061 aggcaatttc acatgaaccg ccccggaaat cctggag
//
LOCUS       DAZKHC020000096        15082 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZKHC020000096 DAZKHC020000000
VERSION     DAZKHC020000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.096"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            128..670
                     /locus_tag="ACHOSM_RS18390"
                     /old_locus_tag="pgaptmp_003678"
     CDS             128..670
                     /locus_tag="ACHOSM_RS18390"
                     /old_locus_tag="pgaptmp_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4560105.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            751..1425
                     /locus_tag="ACHOSM_RS18395"
                     /old_locus_tag="pgaptmp_003679"
     CDS             751..1425
                     /locus_tag="ACHOSM_RS18395"
                     /old_locus_tag="pgaptmp_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4560106.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            1441..3921
                     /locus_tag="ACHOSM_RS18400"
                     /old_locus_tag="pgaptmp_003680"
     CDS             1441..3921
                     /locus_tag="ACHOSM_RS18400"
                     /old_locus_tag="pgaptmp_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGU4560107.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RFDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDNKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSLSLSTLW
                     RDYWGRGGSSKDYQRSYSNNWRRISYTLAASQAYDENHHEEKHFNIFISIPFDWGDDV
                     STPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVIYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3937..4971
                     /locus_tag="ACHOSM_RS18405"
                     /old_locus_tag="pgaptmp_003681"
     CDS             3937..4971
                     /locus_tag="ACHOSM_RS18405"
                     /old_locus_tag="pgaptmp_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HGU4560108.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHDFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAGNNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(5053..5391)
                     /gene="rcnB"
                     /locus_tag="ACHOSM_RS18410"
                     /old_locus_tag="pgaptmp_003682"
     CDS             complement(5053..5391)
                     /gene="rcnB"
                     /locus_tag="ACHOSM_RS18410"
                     /old_locus_tag="pgaptmp_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HGU4560109.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5613..6458)
                     /gene="rcnA"
                     /locus_tag="ACHOSM_RS18415"
                     /old_locus_tag="pgaptmp_003683"
     CDS             complement(5613..6458)
                     /gene="rcnA"
                     /locus_tag="ACHOSM_RS18415"
                     /old_locus_tag="pgaptmp_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HGU4560110.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHHHDHEHHQDHEHHHDHGHHHHHEHGEYQDA
                     HARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLV
                     VSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHG
                     FMGIM"
     gene            6579..6851
                     /gene="rcnR"
                     /locus_tag="ACHOSM_RS18420"
                     /old_locus_tag="pgaptmp_003684"
     CDS             6579..6851
                     /gene="rcnR"
                     /locus_tag="ACHOSM_RS18420"
                     /old_locus_tag="pgaptmp_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HGU4560111.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6943..7039
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7074..7862
                     /gene="thiM"
                     /locus_tag="ACHOSM_RS18425"
                     /old_locus_tag="pgaptmp_003685"
     CDS             7074..7862
                     /gene="thiM"
                     /locus_tag="ACHOSM_RS18425"
                     /old_locus_tag="pgaptmp_003685"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGU4560112.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7859..8659
                     /gene="thiD"
                     /locus_tag="ACHOSM_RS18430"
                     /old_locus_tag="pgaptmp_003686"
     CDS             7859..8659
                     /gene="thiD"
                     /locus_tag="ACHOSM_RS18430"
                     /old_locus_tag="pgaptmp_003686"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGU4560113.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8724..9542
                     /locus_tag="ACHOSM_RS18435"
                     /old_locus_tag="pgaptmp_003687"
     CDS             8724..9542
                     /locus_tag="ACHOSM_RS18435"
                     /old_locus_tag="pgaptmp_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGU4560114.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            9594..10340
                     /locus_tag="ACHOSM_RS18440"
                     /old_locus_tag="pgaptmp_003688"
     CDS             9594..10340
                     /locus_tag="ACHOSM_RS18440"
                     /old_locus_tag="pgaptmp_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4560115.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10314..11279)
                     /locus_tag="ACHOSM_RS18445"
                     /old_locus_tag="pgaptmp_003689"
     CDS             complement(10314..11279)
                     /locus_tag="ACHOSM_RS18445"
                     /old_locus_tag="pgaptmp_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGU4560116.1"
                     /translation="MSGARLHTLLPELTTRQSVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMTKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11276..12280)
                     /locus_tag="ACHOSM_RS18450"
                     /old_locus_tag="pgaptmp_003690"
     CDS             complement(11276..12280)
                     /locus_tag="ACHOSM_RS18450"
                     /old_locus_tag="pgaptmp_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGU4560117.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLERKGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(12277..13553)
                     /locus_tag="ACHOSM_RS18455"
                     /old_locus_tag="pgaptmp_003691"
                     /pseudo
     CDS             complement(12277..13553)
                     /locus_tag="ACHOSM_RS18455"
                     /old_locus_tag="pgaptmp_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
     gene            13810..14862
                     /gene="fbaB"
                     /locus_tag="ACHOSM_RS18460"
                     /old_locus_tag="pgaptmp_003692"
     CDS             13810..14862
                     /gene="fbaB"
                     /locus_tag="ACHOSM_RS18460"
                     /old_locus_tag="pgaptmp_003692"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HGU4560118.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
BASE COUNT         4049 a         3507 c         3776 g         3750 t
ORIGIN      
        1 tggttaattt gttttaaata aataaaacaa tatgtttaca ttgcggcaac tttttgatgt
       61 gagaaatttg tttccaagag atacagactc ttaacaacca aaaataaatt ttattaagga
      121 acatattatg aaacgttcaa ttattgctgc cgctgtcttt tcttcttttt ttatgagcgc
      181 tggagtattt gctgcagacg ttgataccgg aacattaacc attaaaggaa atattgcaga
      241 atctccgtgt aaattcgaag cgggtggtga ttcagtaagt attaatatgc cgactgtacc
      301 aaccactgtc tttgaaggta aagctaaata ttctacctat gatgatgcag tcggtgtaac
      361 cagcagcatg ttaaaaatta gctgcccgaa agaagttgct ggtgtaaaac tctcgttgat
      421 cacaaacgac aaaataaccg gtaacgataa ggcgatagcc agtagcaacg ataccgtggg
      481 ttactatctc tatttaggtg ataacagcga tgtcctggat gtttctgcac cttttaacat
      541 tgagagttat aaaacagcgg aaggtcaata tgctattccg tttaaagcaa aatacctgaa
      601 actgacagat aactcagtgc aatcaggtga tgtgttatct tctctggtta tgcgtgtggc
      661 gcaggattaa taaataaaat tgcaagtttc tccaggtaat aaatatggcc gctatcccca
      721 tggcggcttt ttaatttaag aggcattaag atgaaaggat tattatcttt actcattttt
      781 tctatggtcc ttcctgcaca tgccggaatt gttatctacg ggacgcgcat tatttacccg
      841 gcagaaaata aagaagttat ggtgcagttg atgaaccagg gaaatcgttc ttcgctgctg
      901 caggcgtgga ttgatgatgg cgatacgtcg ttaccaccag aaaaaattca ggttcctttc
      961 atgttaacgc caccagttgc aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa
     1021 attatgccga ataaactgcc cactaataaa gaaagcattt tttatctgaa tgttctggat
     1081 attccaccaa atagtccaga acaagaaggt aagaatgcac tgaagtttgc gatgcaaaac
     1141 agaatcaaat tgttttaccg gccggcgggt attgctccgg taaataaagc gacctttaaa
     1201 aagttactgg taaatcgcag tggcaatgga ttggtgataa aaaatgactc agctaattgg
     1261 gtgacgattt cggatgtcaa agctaataat gtcaaagtca attatgaaac tataatgatt
     1321 gcccccttag aaagtcagag tgttaatgtc aaaagtaata atgcaaataa ctggtatctg
     1381 accattatcg atgaccacgg caactatatt agtgacaaaa tttaattgca ggagctgcct
     1441 atgttgagaa tgaccccact tgcatcagca atcgtagcgt tattgctcgg cattgaagct
     1501 tatgcagctg aagaaacctt tgatacccat tttatgatag gtggaatgaa agaccagcag
     1561 gttgcaaata ttcgttttga tgataatcaa cccttaccgg ggcagtatga tatcgatatt
     1621 tatgtcaata agcaatggcg cgggaaatat gagattattg ttaaagacaa cccgcaagaa
     1681 acatgtttat caagagaagt tatcaagcgg ttaggcatta atagcgataa cttcgccagc
     1741 ggtaagcaat gtttaacatt tgagcaactt gttcagggtg ggagctatac ctgggatatc
     1801 ggggtttttc gtctcgattt cagtgtcccg caggcctggg tggaagaact ggaaagtggc
     1861 tatgttccac cggaaaactg ggagcggggt attaatgcgt tttatacctc ttattatgtg
     1921 agtcagtatt acagtgacaa taaagcgtcg ggtaatagta agagtacata tgtacgtttt
     1981 aacagcgggt taaatttact ggggtggcaa ctgcattctg atgccagttt cagtaaaaca
     2041 aataacaatc caggggtgtg gaaaagcaat accctgtatc tggaacgtgg atttgcccaa
     2101 cttctcggca cgcttcgcgt gggtgatatg tacacatcaa gcgatatttt tgattctgtt
     2161 cgcttcagcg gtgtgcggtt gtttcgtgat atgcagatgt tgcctaactc gaaacaaaat
     2221 tttacgccac gggtgcaggg gattgctcag agtaacgcgc tggtaactat tgaacagaat
     2281 ggttttgtcg tttatcagaa agaggttcct cctggcccgt ttgcgattac agatttgcag
     2341 ttggccggtg gtggagcaga tcttgatgtc agcgtgaaag aggcggacgg ctcggttacc
     2401 acctatctgg tgccttatgc agcggtgcca aatatgctgc aacccggcgt atcgaaatat
     2461 gattttgcgg cgggtcgtag ccatattgaa ggggcgagca aacaaagtga ttttgtccag
     2521 gcgggttatc agtatggttt taataattta ttgacgctgt atggtggctc gatggtcgcg
     2581 aataattatt acgcgttcac tttggggact ggctggaata cacgcattgg tgccatttcc
     2641 gtcgatgcca cgaagtcgca tagtaaacaa gacaacggcg atgtgtttga cgggcaaagt
     2701 tatcaaattg cctacaacaa atttgtgagc caaacgtcga cgcgttttgg tctggcggcc
     2761 tggcgttatt cgtcgcgtga ttaccggaca tttaacgatc acgtttgggc aaacaataaa
     2821 gataattatc gccgtgatga aaacgatgtc tatgacattg ccgattatta ccagaacgat
     2881 tttggccgta aaaatagctt ttctgccaat atgagccagt cattgccaga aggctggggg
     2941 tctttgtcct taagtacttt atggcgtgat tactgggggc gtggtggcag tagtaaagat
     3001 tatcaacgga gttattccaa taactggcgg cggataagct ataccctcgc ggcaagccag
     3061 gcgtatgacg agaatcatca tgaagagaaa cattttaata tttttatatc gattcccttt
     3121 gattggggtg atgacgtttc gacgcctcgt cggcaaatat atatgtctaa ctcaacgacg
     3181 tttgatgatc aggggtttgc ctcaaataat acgggattat caggaaccgt tggaagccgg
     3241 gatcagttta actatggggt caacctgagt tatcagcatc agggaaatga aacgacagct
     3301 ggggcgaatt tgacctggaa cgcgccggtt gcgacagtga atggcagtta tagtcagtcg
     3361 agtacttatc gacaggctgg agccagtgtt tcagggggca ttgtcgcctg gtcgggtggc
     3421 gttaatctgg cgaaccgtct ttccgaaacg tttgctgtga tgaatgcgcc aggaattaaa
     3481 gatgcttatg tcaatgggca aaaatatcgc acaacaaacc gtaatggagt ggtgatatac
     3541 gacggaatga caccttatcg ggaaaatcac ctgatgctgg atgtgtcgca aagcgatagc
     3601 gaagcagaat tacgtggcaa ccggaaaatt gccgcccctt atcgcggcgc ggttgtactg
     3661 gttaattttg ataccgatca gcgcaagccc tggtttatta aagcgttaag agcggatggt
     3721 caaccattaa tgtttggtta tgaagtcaat gatatccatg gtcataatat tggcgttgtc
     3781 ggccagggaa gccagttatt tattcgcacc aatgaagtac cgccatcggt taatgtggca
     3841 attgataagc aacaaggact ttcatgcaca atcaccttcg gtaaagagat tgatgaaagt
     3901 agaaattata tttgccagta atagtcaggt ggttctatgg aaattcgcat aatgctattt
     3961 atattaatga tgatggttat gcctgtgagc tatgcggcat gttatagtga gttatctgtt
     4021 cagcacaatt tggttgttca gggggatttt gcacttactc aaacacaaat agcgacatat
     4081 gagcatgatt ttaatgattc gtcatgcgtg agtacaaata ctatcacccc tatgagttcg
     4141 tcggatatta ttgttggact ttataacgat accataaaat taaatttaca ttttgaatgg
     4201 accaataaaa acaacatcac gttgtcaaat aatcagacca gtttcaccag tggttattca
     4261 gttacggtga cacctgcggc cagtaatgca aaagtgaatg tttctgcggg gggcggcggt
     4321 tcagtgatga ttaatggtgt tgcgacatta tccagtgctt catcatcgac acgcgggagt
     4381 gccgcagtac aatttctact gtgtttatta ggtggcaagt catgggatgc atgtgtaaat
     4441 agctacagaa atgcattggc acaaaatgca ggtgtctatt cctttaatct gacattgtca
     4501 tacaacccga taaccacaac ctgtaaaccg gacgatttat taattacttt agacagtatt
     4561 cccgtttcac aattaccagc cacaggtaac aaagcaacaa ttaatagtaa acaaggggat
     4621 attattctgc gttgtaaaaa tttattaggt caacaaaatc aaacatcacg gaaaatgcag
     4681 gtgtatttat caagttctga tttgttagcc ggcagtaaca cgatcctgaa aggtgcagga
     4741 aataatggag tcggatttat gcttgaaagc aatggttctc cagtcacatt tttaaatatc
     4801 actgataaca gcaaaggata cacaaattta aaagaaattg cggcaaagtc aaaacttaca
     4861 gatacaacgg tttcaattcc gataactgcc agttactacg tctacgatac aaacaaagtt
     4921 aaatctggcg cactggaggc aaccgcatta atcaacgtga aatacgacta ataacaagca
     4981 agacgagcaa gtggctaata ataaaaaata acaaggtgtc tgcatttccc cctcatgatg
     5041 agggggcttt ttttagcgat gataaaaaat ctcaccgtcg taggctttaa tgattttacc
     5101 gtcggtgtcg ctgatcaaca cgtacgcgcc acccatatag gtccagtgcg tcccggcatc
     5161 aggcgcgggc aggttacgta actgccactg cttaatgttg tactcatcgg tgcggtacat
     5221 ctctgggacg gtatcacctg gtttgaaatg cttaaagtca gcaatgaaac tacttaactc
     5281 atacttagaa attcccgagg tattggtcga acccgcggtg gctggtgcgg cccaggcggc
     5341 actggtaacc agcaaaagca cacccagcaa catcttattt ttaatggtca taaactctcc
     5401 actttgggga tctgtaatat cagtcgcata tctttcctgc catacaatat aactgacaag
     5461 cgatattttg ttagtgatcc catgatttcg taaaaagtgt aaatgaaagg atgccctaaa
     5521 agcatcctta gcgagcagag ggtcagcgga aatagctgat ttaagaatca tcctgggctg
     5581 aagataacat gcaagcctaa aggattgagt tatcacatta tgcccatgaa gccatgtacg
     5641 cccatataca caccgactaa gccaatcaac agactggaaa aatagggggc gcgtttagcg
     5701 agagtgttaa atccgctcca gcgttttgcg acctgctgaa cgctgattgc tgcgccaacg
     5761 cctacggtga caagcgttaa cgccaggcca atgctgaaac tgacgaccag tgttgcgccc
     5821 agtgtcaggg ctttcaactg aatgcatatc aatagcacgg taattgctgc cgggcagggg
     5881 ataaggccac cggttaagcc aaataacaaa atttgccagt tggtgacctc tctaccatca
     5941 aagcgtcgtt taatgtcatt ggcatgggct cgtgcatggg catcctgata ctcgccatgt
     6001 tcgtgatggt gatgatgtcc atggtcgtgg tgatgttcgt ggtcttgatg atgttcgtga
     6061 tcgtgatgat ggtgatgatg ctcataatca tactcgtgca tattctccag ccagttgcgt
     6121 tcgccgcgcc aggtacgcca gaacatccag aacgcggtgc caataatgat cactgcggaa
     6181 atcagctgga gccacggttc tgctgattga gcagtaaagc gcttgctgat caccatcccg
     6241 ccaaaggcaa ttaaccagac cactgcggta tgcgaaatag ttgctgccag tccgagcatt
     6301 accgcttgtt taatggtgcc tttgatggcg atgataaacg ccgccatcat cgtttttgag
     6361 tgccctggtt ccaggccatg aagcgcacca agtaagatgg cgctggggat gaagaaccag
     6421 gcgtttcctt gctgaagaag agttgtaaat tcggtcatga taataattct tagtattaat
     6481 tcggcaatct gattctactc ccccccagta cctgatacta ccccccagta gattaatagt
     6541 gctatgattt ttcatattct tgttaaccag gtgttgccat gtctcataca atccgtgata
     6601 aacagaaact gaaagcgcgt gccagtaaga ttcagggcca ggtcgtggcg ctcaagaaaa
     6661 tgctcgacga gccgcacgaa tgcgctgcag ttttacaaca gattgctgct atccgtggcg
     6721 cggtaaacgg tctgatgcgg gaagtgatta aaggtcatct gacggaacac atcgttcacc
     6781 agggggatga gctaaaacgt gaagaagatc tggatgtcgt tctgaaggtg ctggattcat
     6841 atatcaaata atttattaac gcgattgtaa aactgccgtt tttcctcgtt tacaacgcgt
     6901 gcgctggaca ttaccatcct cctctgcgat ttatcatcgc aaccaaacga ctcggggtgc
     6961 ccttctgcgt gaaggctgag aaatacccgt attacctgat ctggataatg ccagcgtagg
     7021 gaagtcacgg accaccaggt cattgcttct tcacgttatg gcaggagcaa actatgcaag
     7081 tcgacctgct gagttcagcg caatctgcgc acgcgttaca cctttttcac caacattccc
     7141 ctcttgtgca ctgcatgacc aacgatgtgg tgcaaacctt tactgccaat accttgctgg
     7201 cgctcggtgc atcgccagcg atggttatcg aaaccgaaga ggccagtcag tttgcggcta
     7261 ttgctagtgc gttgctgatt aacgttggca cgctgacgca gctacgcgca cagtcgatgt
     7321 gtgccgccgt tgagcaagca aaaagctctc aaacaccctg gacgcttgat ccagtagcgg
     7381 ttggtgcact cgattatcgc cgccgttttt gtctggaact tctgtctcat aagccaaccg
     7441 ccatacgtgg taatgcttcg gaaatcatgg cattagctgg cattgctaat ggtggacggg
     7501 gagtggatac cactgacgcc gcagctaacg cgatacccgc tgcacaaaca ctggcacggg
     7561 aaactggcgc aatcgtcgtg gtcactggcg agatggatta tgttaccgat ggacatcgta
     7621 tcattggtat tcacggtggc gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat
     7681 tatcggcggt tgtcgctgcc tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat
     7741 ctgcctgtca ctggatgaaa caagccggag aacgcgcagt cgccagaagc gaggggccag
     7801 gcagttttgt tccacatttc cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat
     7861 gaaacgaatt aacgctctga cgattgccgg tactgatccg agtggtggtg cggggattca
     7921 ggccgatctt aaaaccttct cggcacttgg cgcttatggt tgctcagtta ttactgcact
     7981 ggtggcgcaa aatacccgtg gcgtacagtc ggtgtatcgc attgagcctg attttgtcgc
     8041 cgcccagctc gattcggtgt tcagcgatgt gcgaatcgac accactaaaa tcggtatgtt
     8101 ggcggaaacc gatattgttg aagcggtggc agaacggttg caacgttatc agatccaaaa
     8161 cgtggtactc gacaccgtta tgctggcaaa aagcggcgac ccgctgcttt caccttcggc
     8221 ggttgctacg ctgcgcagtc gattattgcc acaggtttca ttaataacgc caaacttgcc
     8281 cgaagctgcc gccttgctcg acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca
     8341 agggcgatcg ctgttggcga tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga
     8401 tgatgagcaa agcccggact ggctgtttac ccgcgagggt gaacaacggt ttaccgcacc
     8461 gcgcattatg accaaaaaca cccacggcac tggttgtacg ctctctgcgg cgttggctgc
     8521 actacgcccg cgccatacaa actgggctga caccgtacag gaggcaaaaa gctggctttc
     8581 atcagcatta gcccaggccg acacgctgga agttggtcac ggtattggtc cggttcacca
     8641 cttccacgcc tggtggtgag caccgtacta tagctgcttt ggttattgtc agcctttggc
     8701 actgacattc agggatgaaa ccaatgcagc taagaattac cagtcgaaaa aaactcacct
     8761 ctctattatg cgctcttggg ctaatttcca tcgttgcaat ttatccgcgt cagacggtga
     8821 attttttcta ttcgacagca gttcagatta cagactacat ccatttctac ggttatcgcc
     8881 cggttaaatc tttcgctatt cgcattcctg ccagttacac cattcacgga atagatgttt
     8941 ctcgctggca ggagcggatc gactggcagc gggtggcaaa aatgcgcgac aacggtatcc
     9001 gcttacagtt tgcttttatt aaggcgacgg aaggcgaaaa gctggtggac ccgtattttt
     9061 cgcgtaactg gcaactaagc cgcgaaaatg gcctgctgcg cggggcgtat cattatttct
     9121 ccccgtcggt agcagcgccg gttcaggcac gattatttct gcaaacggtg gatttctcac
     9181 aaggcgattt ccctgcggta ctggacgtag aagaacgggg aaaattatcg gcaaaagaat
     9241 tacgcaaacg agtaagtcag tggctaaaaa tggtcgaaaa aagtacggga aaaaagccga
     9301 ttatttactc aggagccgtt ttttatcaca ccaatctggc gggctatttc aatgaatatc
     9361 cgtggtgggt ggctcactat tatcaacgtc gtccggacaa tgacggcatg gcctggcgct
     9421 tctggcagca ttccgaccgt ggacaggtag atggcatcaa tggtccggtg gattttaatg
     9481 tatttaatgg cacggtggag gagttgcagg cattcgttga tgggattaaa gaaacgcctt
     9541 aaataagaag cagtgatata ctcccaccag actccccggc aggaaaaaga caaatggaac
     9601 aagcgcatac ccagctgatt gcccaactga acgagcgtat tttagcggcg gataacacgc
     9661 cgctttatat taagtttgcc gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc
     9721 atggcaatat tttgcccggt gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta
     9781 cggtgcgcaa ggcgatgcag gcgctggaag aagagggtgt ggtgacgcgt tcgcgcggtt
     9841 acggcacgca gatcaacaac atcttcgaat actcgttgaa agaagcgcga ggtttttctc
     9901 agcaggtggt attgcgcggg aaaaagcccg atacgctatg ggttaacaag cgtgtcgtga
     9961 aatgtcccga agaagtcgcg cagcagctgg cggtcgaagc aggaagtgat gtctttttgc
    10021 ttaagcgtat tcgctatgtc gatgaagaag cggtatcgat tgaggaatcg tgggttccgg
    10081 cgcatttaat tcatgatgtt gatgccatcg ggatttcact ttatgactat ttccgcagcc
    10141 agcacattta cccacaacgt acgcgttccc gcgttagcgc ccggatgccg gatgccgagt
    10201 ttcagtcaca tattcagtta gatagcaaaa taccggtgct ggtgatcaag caagtggcgc
    10261 ttgaccaaca gcaacggcct attgagtaca gcattagcca ctgtcgcagc gatctatacg
    10321 tttttgtgtg cgaggagtag ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg
    10381 acaacccacg acgccactgc attgcccagt aatacggcat ccgccagtgg cagaccagag
    10441 gccagcccgg caagtacgcc accggcatga ctgtcgcccg ccccaatggt gtcgacaact
    10501 tgcgttggaa atgccggaat gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct
    10561 ttatcgagac gaacgatcaa cggcgcggca aatttctcct gccattgctt gccaagtgtt
    10621 gttatctctg cggataaagc aaaacgttcg gcggcaatct cagcctcttg acgattgagt
    10681 gacactaaag gtcgacaggc catgatccgc gccagtaatg cgtccgggat atcgccaata
    10741 cgtgggccaa aatcgataaa tggcgtcacg tcttgcagct cttccagcca ttccactaac
    10801 aattcgccgc agggcgaggc cagttgataa ccggaaaaat agagcaggct gccaggcgca
    10861 acggttaatc gcgccagcca ctggcgattc cactgatttt ccacaccgct gaatgacata
    10921 aaagtacgtt cgccatccgg ctcaaccagc gccagacacc agccgttatc gccttcggcg
    10981 ttatcgatca ggctgattaa cccctctttc gtcatccggt tgcgaataat ctccgcccat
    11041 accccctgac cgagcggcaa ggcattaccc gcttcgatgc cgaggcgctt taacgccacg
    11101 gcaatattca gtgcgcagcc gccaacgtta acgctctgct gtttcagttc gatatcgcac
    11161 ccacgccagg ggagggcata agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc
    11221 atcaccgact gacgcgtggt taattcaggc agcagcgtgt gtaatcgagc gccgctcata
    11281 ccgcctcccg ttgttgacga tatttcgcca gcgctgtggc atagcggttg aaatcaagct
    11341 gatttaccgc atccagctcc gcctttaacg caggatcgat agcgttaatg ccatgcaacg
    11401 cgccgcagat tgccgtcgcc atagcaccga tggtgtctgt atcgccgcca aggttagcgc
    11461 agaggacggc gcaacggttc gggtcggttt gtgccagttc aaccagcgca atggcgcacg
    11521 gaacggactc aatagtgctg gtacctgcgc caacgacctg gtaaagctgt tcgctggcgg
    11581 attcggtgcc gtcggcattg cgcacaattt tcagcgcaat ctccagccgc gccgccagtg
    11641 aggcgctgaa cgtggtggtg cgtttttgct gggcatgtcg cgcaattgaa ggcagtgaat
    11701 caacaatcgc tgaccagctt tctccgtcaa tagcacgaga aatcgcccat gcgatgacta
    11761 ccgcacctgc aactgccaga tcggatttat gggtcgggct cgacgccagc gccacatcat
    11821 caataaagga atcaacatcg cgcgccggaa gcaaacaacc taatggcgag acgcgcatcg
    11881 ctgcgccgtt ggttacgccg ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg
    11941 cgttaagcgc aatcttcgag gtcggaccta gcacgttttt gttgaaggcg tcgaaacgca
    12001 gcgcccagtc gaggatatta cgcccaatca gatccggatc gatcttgcct ttacgttcca
    12061 gcaacgcatc cgccagacac agcgccatcg aggtatcgtc ggtgaattcg gcgcggttaa
    12121 aataacaggc cgcgttattc tcctttggtc caggcagaaa acggtcaatc cagccaaagt
    12181 gtgctttaac gcggctgcgt ggccaaagct cggagggcat ccccatcgca tcccctaacg
    12241 cctgcccata aagagcaccg agaatacgtt ctgttttcat ttaacttccc cttgtgtcaa
    12301 cgcaatatcg cgatcatcga ccttgatagc cgtaatttcg ttgtcggatt cgcggaaaaa
    12361 aatcatgaac agcacggcga taatggcaat catcaccgcg ccaaaagtcc acatcccgga
    12421 ccagttgaaa gtcagtccgt ttaccggttc ctgataagcg aacatctttt ccatcatcac
    12481 accgccaaga cgatagccga gcaaactgcc gaagccctgg cagcagagcg tgatcagccc
    12541 ctgcgcggtg gtacgcatat gcacgggggc ttttttatcg acatagatgt aagcggtaac
    12601 gtagtaaaaa tcgtaactta cgccgtgaag caaaataccg aggaacagta acgcgtaggt
    12661 gaaatattca tccgcactac cgtaaataaa gaagccatag cggatcgcag cggtgaccag
    12721 accaagcaat aatacctttt tgataccaaa gcgtttagtg aaaaacggta atgccagcat
    12781 aaagaatatt tcagagaact ggccgagcgt catccagccg gtggcatttt tcatgccgac
    12841 ttcggtcaga taaccgttgg caaagatgta atagaacgcc agtggcatcg caaacaggaa
    12901 tgaacagaag aaaaagacga ggaagttttt atcgcgcagc aggatcagcg catccaggcc
    12961 gagcatgact ttaatatcca ttttgccggt gctttttggt ggcgtgtcgg gcaggaaaaa
    13021 cgcaaacaca ccgagcagag cagaacttcc ggcggtaatc agcagcggga tgttagtcgg
    13081 tgagatatcg gcatacccca gtatttgcgg caagaaacca catgccagac cggaggcaat
    13141 ccagccgata gtgcccatca cacgaatgcg cgggaaatca cgctccacat ccggcacgtt
    13201 ggcaaaagcg atgctgttag tcagcgcaat ggtcggcata taggttagag agtaagccag
    13261 cagtaacgga aagaacccgg caaaagtggt ctgttgcgca gcgaaataca tcagcagcgc
    13321 gcctgcgaac atcaataccg ccagcacttt ttgcgccgag aaaaagcggt cagtgatgga
    13381 gccaaccaga attggcgaca ggatcgccgc aatggcggta caggcatacg accagccaat
    13441 ttctccggca ctaaaaccgc ttttacttaa ccacaaccac aatggcacaa accacgcgcc
    13501 cagataaacc attcaacaaa catcatgaac gacagctttg ctgttgtttt catcttttaa
    13561 ttccttcatg acaggtaatg taaggtacgc gatgacaata ccacttaata atacctttta
    13621 aatacctttg cggtgaaaac ttgaccgcgc taacattttt tctgatgaat cgagccaaca
    13681 gaaaacgctg ataaaacatc caaaagatgg aaaaactcgc ccgtaacgac caggattatg
    13741 attgcccgca aaaggcgggc caggacaatc catagccgat atccaatcgg aatttacggg
    13801 agcatagtaa tgacagatat tgcgcagttg cttggcaaag acgccgacaa ccttttacag
    13861 caccgttgta tgacaattcc ttctgaccag ctttatctcc ccggacatga ctacgtagac
    13921 cgcgtaatga ttgacaataa tcgcccgcca gcggtgttac gtaatatgca gacgttgtac
    13981 aacaccgggc gtctggctgg cacaggatat ctttctattc tgccggttga ccagggcgtt
    14041 gagcactctg ccggagcttc atttgctgct aacccgctct actttgaccc gaaaaacatt
    14101 gttgaactgg cgatcgaagc gggctgtaac tgtgtggcat caacttacgg cgtgttggcg
    14161 tcggtatcgc ggcgctatgc gcatcgcatt ccatttctcg tcaaacttaa tcacaacgag
    14221 acgctaagtt acccgaatac ctacgatcaa acgctgtatg ccagcgtgga gcaggctttc
    14281 aacatgggcg cggtggcggt tggtgcgact atctattttg gctcggaaga gtcacgtcgc
    14341 cagattgaag aaatttctgc ggcttttgaa cgtgcgcatg agctgggcat ggtgaccgtg
    14401 ctgtgggcct atttgcgtaa ctccgccttt aagaaagatg gcgttgatta ccatgtttcc
    14461 gccgacctga ccggtcaggc aaaccatctg gcggcaacca tcggcgcaga tatcgtcaaa
    14521 caaaaaatgg cggaaaataa cggcggctat aaagcgatta attacggtta taccgacgat
    14581 cgcgtgtaca gcaagttaac cagcgaaaac ccgattgatc tggtgcgtta tcagttagct
    14641 aactgctata tgggccgggc tgggttgata aactccggcg gtgctgcagg cggtgaaact
    14701 gacctcagtg atgcagtgcg tactgcggtt atcaacaaac gcgctggcgg aatggggctg
    14761 attcttggac gtaaggcgtt caagaaatcg atggctgacg gcgtgaaact gattaacgcc
    14821 gtgcaggacg tttatctcga tagcaaaatt actattgcct gatacgcctt atacgaacaa
    14881 caccaccccg taggacggat aaggcgttta cactgcatcc ggcaacctat gcctgatgcg
    14941 acgcggaagc gtcttatcat gcctacatag cactgccacg tatgtttaca ccgcatccgg
    15001 cataaaaaca cgcgcacttt gctacggctt ccctatcggg aggccgtttt tttgcctttc
    15061 actcctcgaa caattttcat at
//
LOCUS       DAZKHC020000097        15065 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZKHC020000097 DAZKHC020000000
VERSION     DAZKHC020000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.097"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(116..191)
                     /locus_tag="ACHOSM_RS18465"
                     /old_locus_tag="pgaptmp_003693"
     tRNA            complement(116..191)
                     /locus_tag="ACHOSM_RS18465"
                     /old_locus_tag="pgaptmp_003693"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Phe,seq:gaa)
     gene            complement(297..1004)
                     /gene="yqgA"
                     /locus_tag="ACHOSM_RS18470"
                     /old_locus_tag="pgaptmp_003694"
     CDS             complement(297..1004)
                     /gene="yqgA"
                     /locus_tag="ACHOSM_RS18470"
                     /old_locus_tag="pgaptmp_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HGU4560119.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1157..1240
                     /gene="yqgH"
                     /locus_tag="ACHOSM_RS18475"
                     /old_locus_tag="pgaptmp_003695"
     CDS             1157..1240
                     /gene="yqgH"
                     /locus_tag="ACHOSM_RS18475"
                     /old_locus_tag="pgaptmp_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HGU4560120.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            1402..3537
                     /gene="speC"
                     /locus_tag="ACHOSM_RS18480"
                     /old_locus_tag="pgaptmp_003696"
     CDS             1402..3537
                     /gene="speC"
                     /locus_tag="ACHOSM_RS18480"
                     /old_locus_tag="pgaptmp_003696"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HGU4560121.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVEMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3587..4843)
                     /gene="nupG"
                     /locus_tag="ACHOSM_RS18485"
                     /old_locus_tag="pgaptmp_003697"
     CDS             complement(3587..4843)
                     /gene="nupG"
                     /locus_tag="ACHOSM_RS18485"
                     /old_locus_tag="pgaptmp_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HGU4560122.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5045..6124)
                     /gene="mltC"
                     /locus_tag="ACHOSM_RS18490"
                     /old_locus_tag="pgaptmp_003698"
     CDS             complement(5045..6124)
                     /gene="mltC"
                     /locus_tag="ACHOSM_RS18490"
                     /old_locus_tag="pgaptmp_003698"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HGU4560123.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPSLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6189..6464)
                     /gene="yggX"
                     /locus_tag="ACHOSM_RS18495"
                     /old_locus_tag="pgaptmp_003699"
     CDS             complement(6189..6464)
                     /gene="yggX"
                     /locus_tag="ACHOSM_RS18495"
                     /old_locus_tag="pgaptmp_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HGU4560124.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6492..7544)
                     /gene="mutY"
                     /locus_tag="ACHOSM_RS18500"
                     /old_locus_tag="pgaptmp_003700"
     CDS             complement(6492..7544)
                     /gene="mutY"
                     /locus_tag="ACHOSM_RS18500"
                     /old_locus_tag="pgaptmp_003700"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGU4560125.1"
                     /translation="MQASQFSVQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7705..8424
                     /gene="trmB"
                     /locus_tag="ACHOSM_RS18505"
                     /old_locus_tag="pgaptmp_003701"
     CDS             7705..8424
                     /gene="trmB"
                     /locus_tag="ACHOSM_RS18505"
                     /old_locus_tag="pgaptmp_003701"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGU4560126.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8424..8750
                     /gene="yggL"
                     /locus_tag="ACHOSM_RS18510"
                     /old_locus_tag="pgaptmp_003702"
     CDS             8424..8750
                     /gene="yggL"
                     /locus_tag="ACHOSM_RS18510"
                     /old_locus_tag="pgaptmp_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HGU4560127.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            8934..9653
                     /gene="yggN"
                     /locus_tag="ACHOSM_RS18515"
                     /old_locus_tag="pgaptmp_003703"
     CDS             8934..9653
                     /gene="yggN"
                     /locus_tag="ACHOSM_RS18515"
                     /old_locus_tag="pgaptmp_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HGU4560128.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9829..10875
                     /gene="ansB"
                     /locus_tag="ACHOSM_RS18520"
                     /old_locus_tag="pgaptmp_003704"
     CDS             9829..10875
                     /gene="ansB"
                     /locus_tag="ACHOSM_RS18520"
                     /old_locus_tag="pgaptmp_003704"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HGU4560129.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10992..11999
                     /gene="yggM"
                     /locus_tag="ACHOSM_RS18525"
                     /old_locus_tag="pgaptmp_003705"
     CDS             10992..11999
                     /gene="yggM"
                     /locus_tag="ACHOSM_RS18525"
                     /old_locus_tag="pgaptmp_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HGU4560130.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(12055..13356)
                     /locus_tag="ACHOSM_RS18530"
                     /old_locus_tag="pgaptmp_003706"
     CDS             complement(12055..13356)
                     /locus_tag="ACHOSM_RS18530"
                     /old_locus_tag="pgaptmp_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HGU4560131.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(13356..13859)
                     /locus_tag="ACHOSM_RS18535"
                     /old_locus_tag="pgaptmp_003707"
     CDS             complement(13356..13859)
                     /locus_tag="ACHOSM_RS18535"
                     /old_locus_tag="pgaptmp_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HGU4560132.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(13904..14890)
                     /locus_tag="ACHOSM_RS18540"
                     /old_locus_tag="pgaptmp_003708"
     CDS             complement(13904..14890)
                     /locus_tag="ACHOSM_RS18540"
                     /old_locus_tag="pgaptmp_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HGU4560133.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKNKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         4006 a         3587 c         3802 g         3670 t
ORIGIN      
        1 ttgatgtggg gagaatttgg ttgagttcgg tagaattgat ttggaggaag aacacttaaa
       61 tcatggtgtt ctgaaacgaa aaacggacct ccgtggaggt ccgttgatat gaatttggtg
      121 cccggactcg gaatcgaacc aaggacacgg ggattttcaa tcccctgctc taccgactga
      181 gctatccggg caacggggcg cattaaacct gattcgcctc gtctcgtcaa tcaaatttct
      241 tcaatttact gcagactgca caatctatca tcactttgcc gctattgcac gcattgtcag
      301 gcaaaccagg cggtccaggc ggcggaaagc ggcattgcca gaaggagtgc tggaagcatg
      361 ttgaccaccg ggaacatttt aatgccacag atgcgtaatc cggttgccag cagcaataaa
      421 ccgcctacgg cgctgaagtc tgccatcatc gacggtgtgg tcagcggtaa tatcagcgcg
      481 gcagcccacg ccagcgttaa ctggatgatc agtaatggga tactaattac cgacaccgca
      541 atacccagcg agcaggcgaa gatcatcgcc gtaaagaaat caagaaatga cttggcgatt
      601 aaaatactcg gatcgccggt catcccttcg ttcatcgccc cgaagatccc ggtgccgctg
      661 gcgcaaaaca ggacaataat cgcgacataa ttctgaataa aagattcatg cgttggcttc
      721 ttacgtgagt gacgaaacag attttgtgct ttggcgactg ctgtattgac acctttctcc
      781 agcaaacaaa tttcgccaat tagagcgcca agtagcgtag ctaaaaccat cgctggaagg
      841 ttggcgcatt tcacaaccag taaaatacca atccccagcg atgccagacc aaaaatagat
      901 gtcatggaga cgcggatacg ttccggtaag cgttggctga gcagtgcgcc gagaacgcca
      961 cccagtaaga ctgcactagc gttgataaaa gggccgatga ccacaagagt tcctgttagc
     1021 tgtattgttt gattttctta ttatgaactt tttgccttgt tgatggcttg tctggtcgca
     1081 aagtcctggc ttgcacgctt tagcgaaagg tgccatgatt gcgcgaattt tctcctctct
     1141 gtacggagtt tgcccgatgc acgccatctc cttacattct ctcgcttatc gccgtttcgc
     1201 gcgaaacgtt tccctttcta tgttactgct catgcggtga agttaacgca cgctcactgc
     1261 aggacaacag taaaatcaga gcgtttctgc ttttactgat gtctggcggt cggagctggt
     1321 gaccagtttg acccatatct catggggcag ggttttccac cttgtccggt attcttactt
     1381 ccccgaaacg ggtttgcgct tatgaaatca atgaatattg ccgccagtag tgaactggta
     1441 tcccgacttt cttctcatcg tcgcgtggtg gcgttgggag atactgattt tacggacgtc
     1501 gcggcagtcg tcattaccgc tgcggatagt cgcagtggca ttcttgcgtt gcttaagcgc
     1561 accggttttc atctaccggt gtttttgtat tccgaacatg ctgttgaatt acctgcgggc
     1621 gttacggcgg taatcaacgg caacgagcag cagtggctgg agctggaatc cgcagcctgt
     1681 cagtatgaag agaatttgct gccaccgttt tatgacacgc tgacgcagta cgttgagatg
     1741 ggcaacagca cctttgcttg ccctggacat caacatggtg cgttttttaa aaagcatcct
     1801 gccggacgcc atttttacga tttctttggt gagaacgtct ttcgcgccga tatgtgtaac
     1861 gctgacgtaa aattgggcga tctgcttatt catgaaggat cggcgaaaga tgcgcagaaa
     1921 ttcgcagcca aagtctttca tgccgataaa acctattttg tgctgaacgg cacatcggca
     1981 gcgaataaag tggtgacgaa tgcgctgtta acgcgtggcg atctggtgct cttcgaccgt
     2041 aacaaccata agtcgaatca tcacggcgcg ctgattcagg cgggggcgac gccggtctat
     2101 ctggaagctt cacgcaaccc gtttggtttc attggcggta ttgatgcgca ctgttttaat
     2161 gaagagtatc tgcgccagca aattcgcgac gttgcgccag aaaaagccga gctgccgcgc
     2221 ccgtttcgcc tggcgattat tcagctggga acctatgacg gaactgtcta taacgcccgt
     2281 caggtgatcg ataccgttgg gcatctgtgt gattacattc tgtttgattc cgcgtgggtc
     2341 ggttacgaac agtttatccc gatgatggcg gatagctcgc cgctgctatt agaacttaac
     2401 gaaaacgatc cggggatctt tgtgactcag tcggtgcaca aacagcaggc gggattctca
     2461 cagacgtcgc agatccataa aaaagataac catatccgcg gacaggcgcg tttttgcccg
     2521 cataagcggt tgaataacgc ctttatgctc catgcttcta ccagcccttt ctatccgctg
     2581 tttgctgcac tggatgttaa cgccaaaatt catgaagggg agagtgggcg tcggctgtgg
     2641 gctgagtgcg ttgagttggg tattgagtcg cgcaaggcta ttcttgcgcg ctgtaagctg
     2701 ttccgcccgt ttatcccgcc cgttgttgat ggcaaattgt ggcaggatta tccgacatca
     2761 gtgttagcca gcgaccgccg ttttttcagt tttgagccgg gggcgaagtg gcacggcttt
     2821 gaaggatatg ccgcggatca gtattttgtt gatccgtgca agctgttact cactacacca
     2881 ggtatcaatg ccgaaaccgg cgaatatagc gactttggcg ttccggcgac gattctggcg
     2941 cactatctgc gtgagaacgg cattgtgccg gagaagtgcg atctcaactc cattctgttc
     3001 ttattaactc cggcggaaag ccacgagaag ctggcacaac tggtagagat gttggcgcag
     3061 tttgaacagc atattgagga tgactcgccg ctggctgagg tgttgccgag cgtttataac
     3121 aaatatccgg tgcgctatcg cgactacact ttgcgccagt tgtgtcagga gatgcacgat
     3181 ctgtatgtca gtttcgacgt caaagaccta caaaaagcga tgttccgcca gcagagtttc
     3241 ccgtcagtgg tgatgaaccc ccaggatgcg catagcgctt atattcgcgg tgaagtggag
     3301 ttggtgcgga ttcgtgatgc cgaagggcga attgcggcag aaggggcgtt gccttatcca
     3361 cctggcgtgc tttgcgtggt acccggggaa gtctggggtg gggcggttca acgttatttc
     3421 cttgcactgg aagaaggggt gaatttgttg ccgggatttt cgccggagct gcaaggtgtt
     3481 tatagcgaaa ccgatgcgga tggcatgaaa cggttgtacg gttatgtgtt gaagtaagaa
     3541 taaaaaaaac gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg
     3601 tctgtgtgcc tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg
     3661 ccagaaccac ggagtaacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt
     3721 tttgggtgta catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc
     3781 cgttagtcat catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt
     3841 tttcgacaaa caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca
     3901 tcgacagtac cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa
     3961 aacgcaggat ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta
     4021 agaagaacgg gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga
     4081 ttgacgcatg ctgcacaata aagctgctgg caaacatcgg atctttgtcg aagctgtgca
     4141 ggaaggtatt accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga
     4201 taaagaagat tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg
     4261 ttgtccagct ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa
     4321 acagaaccag aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga
     4381 agccagacag gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga
     4441 ttggcgggaa gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg
     4501 tgttgattaa cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca
     4561 tcgcctccgg tgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc
     4621 aaatggcata tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag
     4681 gcataaagac cgctgcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa
     4741 acttcagggt aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga
     4801 actgcagaaa agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc
     4861 gtgatgcgga tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc
     4921 aacttcgcgg actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc
     4981 aaaacgaaat tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat
     5041 ggaattatcg gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag
     5101 attccgcaga gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt
     5161 taataatatt ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc
     5221 cgccgttata ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga
     5281 gataaacatt gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat
     5341 caaacaagaa actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc
     5401 cggcagtatg ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg
     5461 ggttaaagga agattcggtc tgcataattg ccagaatcag cgactcatca acgccatatt
     5521 tccgtgacgc ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg
     5581 gtaccatgtt aatggtgacg ctgtagatga tacgcagccc gttactgcgg ctttgcagac
     5641 ggtttttcag cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc
     5701 cggtgttgtc caccacctga ccgtaaaggg aaggttcttt cgaaatcgta atatcatcaa
     5761 cgtcggaata gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg
     5821 cccggcgcaa atgcgcggca ggttctgtcc cggcgatagt ttcgatagta atcgtaccgt
     5881 catcgaagtt gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag
     5941 gaccggcgat caccacctct ttgaatcccc agatgttctc aatattgtgg gcaaattgcc
     6001 ccatcagaat atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt
     6061 ttttggtcgt cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt
     6121 tcatcattcc gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg
     6181 cactgttttt attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg
     6241 aacaggaagt tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg
     6301 agtttctttt cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg
     6361 gagatctcgt tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct
     6421 tctgcttcac gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat
     6481 cgactcacgc gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga
     6541 gccgccaggc caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc
     6601 atgcagccgg tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg
     6661 ctgaaggtat ggcgaaacgc ggtcagctgc gtcaggttat cggcagcaat ctgccgttgc
     6721 gccagccaat gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc
     6781 cacaatccgc tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taatagcaaa
     6841 aagtagccgg tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag
     6901 ctattgttgg cggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggtttc
     6961 gagcgcgtac aaatcatcgc acccaaatcc atcatcgcct gattaaaccg ttccacgcca
     7021 accgcgggcg tcacctgctc gctcaaactc cataatttat tctcgacctc ttttttccca
     7081 ggccagccgc ttacagcata gcagcgcgcc agcacccgtt tgacgttacc gtcgagaatc
     7141 ggaaagtgct tacccagaga aagcgagaga atcgcacctg cggtggaacg cccgacgccc
     7201 ggtaacgccg cgacttcttc aaaggtttcc gggaatttac cgccgtgtaa ggtcgccact
     7261 tgttgtgccg ctttatgcag attgcgcgcg cgggcgtaat agccaagccc ggtccacaag
     7321 tggagaactt cgtccagcgg cgcattggcg agatcggtca ccgtcgggaa gcgcgccata
     7381 aagcgttcaa aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag
     7441 agccatactt tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta
     7501 tcgtaccagt ccagaacctg gactgaaaat tgcgacgctt gcatggtcac cgaattcact
     7561 gttgttgggg gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag
     7621 ccacggcctt atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac
     7681 actttcacaa gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa
     7741 aacggccgcc cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa
     7801 ggccaggaac atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagcgaagat
     7861 atgctggatt tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt
     7921 ggcatggggg cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc
     7981 attgaagtgc attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agagggctta
     8041 agcaacctgc gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac
     8101 aattcattgc gcatggtaca gctctttttc cctgacccgt ggcacaaagc gcgccataat
     8161 aaacgccgta tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg
     8221 ggcgtattcc atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg
     8281 tcttctattg acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca
     8341 tcacgtccgg tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac
     8401 ttaatgttcg agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa
     8461 tgcacatcga cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta
     8521 catcggaaga acagattgat aaaaccgttg atgattttat taacgaggtt atcgaaccga
     8581 acaaactggc ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg
     8641 aaatcggcaa atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagcgca
     8701 aactggatga ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac
     8761 gggcgatgac aaatgcaaaa ctgcctgatg cgctacgctt atcatgcctg gaaagatgca
     8821 cgatcgagta ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg
     8881 ttatcaatct ggggccagca aatgctggcc tgatttgttc ttgagggaag actatgatgc
     8941 gcaaaatgct gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc
     9001 agtgcagcgt cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg
     9061 gcgaaaacgg caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat
     9121 attccctgaa tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca
     9181 cgctaccgtg gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg
     9241 ataaaattat cgttcaggag atgggcgaaa gcagcaaaat gcgcagccgt ctgaccaaac
     9301 ttgatgcgca gctgaaagag cagatgaacc gcattatcga aacgcgcagc gatggcctga
     9361 cgtttcacta taaagccatt gatcaggttc gcgccgaagg ccagcaatta gttaatcagg
     9421 caatgggcgg aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa
     9481 gcggtggtaa cccattacag aatgtgctgg gaagcctggg cggtctgcaa tcctcaatcc
     9541 aaaccgagtg gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc
     9601 gcgttgtgac tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta
     9661 ttttaagacg gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct
     9721 aacgttgtag atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat
     9781 acagagctaa gggataatgc gtagcgttca cgtaactgga ggaatgaaat ggagtttttc
     9841 aaaaagacgg cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc
     9901 aatatcacca ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa
     9961 tctaactaca cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta
    10021 aaagacattg cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac
    10081 gataatgtct ggctgacact ggcgaaaaaa attaacaccg actgcgataa aaccgacggc
    10141 ttcgtcatta cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg
    10201 gtgaaatgcg acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc
    10261 gcagacggtc cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct
    10321 aatcgtggcg tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa
    10381 accaacacca ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt
    10441 cacaacggta agattgacta ccaacgtacc ccggcacgta agcacaccag cgatacgcca
    10501 ttcgatgtct ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac
    10561 gcatccgatc ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct
    10621 ggtgtgggta atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa
    10681 aacggcactg cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct
    10741 gaagtggatg atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg
    10801 cgcgtcctgc tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatc
    10861 ttcaatcagt actaatcacc tcgccccggt atcgtgccgg ggctttttca cttcagactt
    10921 acctccattg ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgtg
    10981 aggtttttcg ggtgaaaaag caatggattg ttggcacggc actgcttatg ttgatgactg
    11041 gtaatgcctg ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa
    11101 agcagtatca cggcattctc aagcttgatg ctatcacctt aaaaaatctt gatgctaagg
    11161 gtaatcaggc cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct
    11221 gggtcggtca gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg
    11281 taaaattctc ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact
    11341 tttactcttt tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga
    11401 ataataaata tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat
    11461 ctgcgttgaa tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg
    11521 ataagcaaat ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg
    11581 gtaagcaaat gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta
    11641 ataaacagaa cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg
    11701 cgattgcggc atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac
    11761 gagatttcga tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc
    11821 gtaaattgca ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag
    11881 tttcagaaat aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag
    11941 ctaatgaacg ctggaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac
    12001 tcatgttaag tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat
    12061 tagccctagt agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa
    12121 cgctactagc aatgcagcat aaaatggcag caatggtttg attaaatgtt gaatcttcac
    12181 acccgagata gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat
    12241 acaaaggttg gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc
    12301 aatcggcaag aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc
    12361 gacaattaat aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc
    12421 catcaatgcg tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc
    12481 cacggaaaaa ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag
    12541 tttgggtaaa tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta
    12601 gacaacggca attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat
    12661 cacaatgaac accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc
    12721 aaaggttgct ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat
    12781 cattaccgca agacccatta agatccctgg caaatatccc gccataaata gcgatgctac
    12841 agaaacgccg ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa
    12901 tccagtaata caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttgttt
    12961 ggtctgaatg ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga
    13021 aatggaacca aacatcatat tcgccaggac gttcacatga ccaagtgaac caggaacccg
    13081 accaaccatt acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag
    13141 cgttccggca aagatgaaaa aggggattgc caatagggca aagttatcca gtccattagc
    13201 cagtcgctgc gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc
    13261 tacagtggca ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa
    13321 acagccaaac agtgtaatcg cgatataact atccattaac tggcctcctg cgcatgttgt
    13381 ttcagttgtt gaatgctttg attaataaaa cacaaggcat aaaacatcat caccaatcca
    13441 cttaatggca gaatgatata tacgtatccc atcggtattt gcatcgcggc agatacctgt
    13501 gatgttgcca gcgttttatc aattaatttc aggccaccag tcacaatgac cgaaccggca
    13561 aaacctaaaa taagaacgtt aatgactata ctaagaagta attgcttgcg cttattcagg
    13621 gcgagagcaa ataaatcgat agagagatgt ctctgcgcgc cgacggtata tgcagcacct
    13681 aataacccta cccagatcat caaaaatcgt gccagctcat cggttaatgt acttggctgg
    13741 ttaagtacgt agcggctaaa tacttgccag actacgcata caaccagagc aagcatgacg
    13801 gcgacagaaa acgcggcaat gacgcggtct accgcaagtt tcaggcggtt aaatatcatt
    13861 gtaagcatcc tggaaaatca gggccagaga ctggcccgga atattattga gcagcgttgt
    13921 cgaacttctc taacaaagca gcttgttttg gatctttttt gaagtcgtca aacagaggct
    13981 gaactgcggc acggaatggt gctttatcca cttttacaat ttcacccccc atagctttcg
    14041 cctgagcacg tgtgtcagcg tcaatttttt cccacaattt ttgttgataa gcttcggatt
    14101 tcttcgctgc tgtttcgagg atttgttgct gttctggggt taatttattc catgttttgg
    14161 tggagataac caaaaagtca ggaattgagg cgtgttcatc ttcagaaaaa actttggcaa
    14221 tttcaatatg tcgagtctgc acccaggaag gcacgttatt ttctgcgcca tctactacac
    14281 cttgctgcat cgcagtataa acttcgccaa atgaaattgg agtgggtgaa ccacccatca
    14341 gttcaatcat tttgatggtg gtcggacttg gctgaacacg aattttcagc cctttaagat
    14401 cttctggctt agtaatcggt tttttcgcat agaaactgcg tgtacccgct acataagctg
    14461 ataaagcaaa aaagcctttg ttttttgttg agtccataat ttctttacca acttcaccaa
    14521 aaacaacttt gttgaagtga gcctgatctt taaataagaa cggcaaatta tagatagcat
    14581 aaatattatc aaaagactcc agatcactgg cggaaccttt ggtcatatcc agagcgccat
    14641 tttgcaacaa ctccatagtt tctcgcgcat tacccatctg gctgctggga taaatacgga
    14701 tcaccatatt accttttgac agctgtttta cctctttcgc cagctcttca aaactctgat
    14761 gtacaacatg acttctttcc aggttgtgtg ctaactttaa cgtcactttt tctgcggcgt
    14821 ttacgccagt gctaaccaga gccagcaagg atgcgcagac taatgagcgg gtaaatgacc
    14881 tcattttcat aaaaaaactc cctgttgaag gatggaaatt ggttgatatc taattgtgct
    14941 tatcagtgct ttgtgcgaca aattggttaa cctgtctgac aacttgtcta tgtttatcac
    15001 aacttgaaat aaagtcagaa attaaatatt aaatttgtgc gccaggttgt acatgtttat
    15061 aatca
//
LOCUS       DAZKHC020000098        14972 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZKHC020000098 DAZKHC020000000
VERSION     DAZKHC020000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.098"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(37..273)
                     /gene="yjjZ"
                     /locus_tag="ACHOSM_RS18545"
                     /old_locus_tag="pgaptmp_003709"
     CDS             complement(37..273)
                     /gene="yjjZ"
                     /locus_tag="ACHOSM_RS18545"
                     /old_locus_tag="pgaptmp_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HGU4560134.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            414..1202
                     /gene="fhuF"
                     /locus_tag="ACHOSM_RS18550"
                     /old_locus_tag="pgaptmp_003710"
     CDS             414..1202
                     /gene="fhuF"
                     /locus_tag="ACHOSM_RS18550"
                     /old_locus_tag="pgaptmp_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HGU4560135.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="ACHOSM_RS18555"
                     /old_locus_tag="pgaptmp_003711"
     CDS             complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="ACHOSM_RS18555"
                     /old_locus_tag="pgaptmp_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HGU4560136.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1875..2600)
                     /gene="yjjQ"
                     /locus_tag="ACHOSM_RS18560"
                     /old_locus_tag="pgaptmp_003712"
     CDS             complement(1875..2600)
                     /gene="yjjQ"
                     /locus_tag="ACHOSM_RS18560"
                     /old_locus_tag="pgaptmp_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4560137.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            3219..3989
                     /gene="yjjP"
                     /locus_tag="ACHOSM_RS18565"
                     /old_locus_tag="pgaptmp_003713"
     CDS             3219..3989
                     /gene="yjjP"
                     /locus_tag="ACHOSM_RS18565"
                     /old_locus_tag="pgaptmp_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HGU4560138.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNQDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            3980..4453
                     /gene="yjjB"
                     /locus_tag="ACHOSM_RS18570"
                     /old_locus_tag="pgaptmp_003714"
     CDS             3980..4453
                     /gene="yjjB"
                     /locus_tag="ACHOSM_RS18570"
                     /old_locus_tag="pgaptmp_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HGU4560139.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNIPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            4560..5099
                     /gene="dnaT"
                     /locus_tag="ACHOSM_RS18575"
                     /old_locus_tag="pgaptmp_003715"
     CDS             4560..5099
                     /gene="dnaT"
                     /locus_tag="ACHOSM_RS18575"
                     /old_locus_tag="pgaptmp_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HGU4560140.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            5102..5839
                     /gene="dnaC"
                     /locus_tag="ACHOSM_RS18580"
                     /old_locus_tag="pgaptmp_003716"
     CDS             5102..5839
                     /gene="dnaC"
                     /locus_tag="ACHOSM_RS18580"
                     /old_locus_tag="pgaptmp_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HGU4560141.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            5888..6382
                     /gene="yjjA"
                     /locus_tag="ACHOSM_RS18585"
                     /old_locus_tag="pgaptmp_003717"
     CDS             5888..6382
                     /gene="yjjA"
                     /locus_tag="ACHOSM_RS18585"
                     /old_locus_tag="pgaptmp_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HGU4560142.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNN
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            6636..8927
                     /gene="opgB"
                     /locus_tag="ACHOSM_RS18590"
                     /old_locus_tag="pgaptmp_003718"
     CDS             6636..8927
                     /gene="opgB"
                     /locus_tag="ACHOSM_RS18590"
                     /old_locus_tag="pgaptmp_003718"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HGU4560143.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(9066..10088)
                     /gene="lgoD"
                     /locus_tag="ACHOSM_RS18595"
                     /old_locus_tag="pgaptmp_003719"
     CDS             complement(9066..10088)
                     /gene="lgoD"
                     /locus_tag="ACHOSM_RS18595"
                     /old_locus_tag="pgaptmp_003719"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HGU4560144.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDLEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRDLI
                     KGVITF"
     gene            10227..11141
                     /gene="lgoR"
                     /locus_tag="ACHOSM_RS18600"
                     /old_locus_tag="pgaptmp_003720"
     CDS             10227..11141
                     /gene="lgoR"
                     /locus_tag="ACHOSM_RS18600"
                     /old_locus_tag="pgaptmp_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HGU4560145.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            11356..12717
                     /gene="lgoT"
                     /locus_tag="ACHOSM_RS18605"
                     /old_locus_tag="pgaptmp_003721"
     CDS             11356..12717
                     /gene="lgoT"
                     /locus_tag="ACHOSM_RS18605"
                     /old_locus_tag="pgaptmp_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4560146.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFHNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(12766..14397)
                     /gene="tsr"
                     /locus_tag="ACHOSM_RS18610"
                     /old_locus_tag="pgaptmp_003722"
     CDS             complement(12766..14397)
                     /gene="tsr"
                     /locus_tag="ACHOSM_RS18610"
                     /old_locus_tag="pgaptmp_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HGU4560147.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALEEQASRLTEAVAVFRIQQ
                     QQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(14516..14962)
                     /gene="hpaR"
                     /locus_tag="ACHOSM_RS18615"
                     /old_locus_tag="pgaptmp_003723"
     CDS             complement(14516..14962)
                     /gene="hpaR"
                     /locus_tag="ACHOSM_RS18615"
                     /old_locus_tag="pgaptmp_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HGU4560148.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         3729 a         3912 c         3551 g         3780 t
ORIGIN      
        1 tatctttact tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattat
       61 catcagcatg atggcagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa
      121 ctgtttgtga tacagcatca ccggcgtcaa tactaatccc atcagaatga caatccgcca
      181 ctgatccggc gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac
      241 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      301 aaccaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
      361 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct
      421 atcgttccgc accgctctat gaagatatca tctggcgaac gcatctccag ccgcaggatg
      481 ctgggcttgc acaagccgta cgtgcgacga tcgccaaaca tcgtgaacat ttgctcgagt
      541 ttatccgcct ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcac
      601 cgaatgcgct aagttctcta ctcgcggtct attccgatca tatctatcgc aaccaaccaa
      661 cgatgatccg cgagaacaaa ccgctgatct cactatgggc acaatggtat atcggcctga
      721 tggtgccacc attaatgctg gcgctactga cgcaggaaaa ggcattagat gtgtcgccgg
      781 aacatttcca tgctgagttt cacgaaaccg gacgcgtcgc ctgtttctgg gtcgatgtgt
      841 gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaacg ttaatcagcc
      901 aggcgctggt tccggttgtg caggcactag aagcgaccgg agagatcaac ggcaaactta
      961 tctggagtaa taccggttat ttgatcaact ggtatctcac tgagatgaag caactgctcg
     1021 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg ctcacgaacg
     1081 gtgaagataa tccactctgg cgtaccgtcg tgctgcgcga tggcctgctg gtgcgccgaa
     1141 cctgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
     1201 gaaagcactg ccggggaagt aaacccggca tcatgcggat taatagggat gcaacacatt
     1261 acttgcttcg caatgccgca tacataacag aatatctgcg gcctccaaca accctgcatc
     1321 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
     1381 gcgtttaagc tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat
     1441 ttctctctcc gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg
     1501 gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
     1561 cgttgacgct ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg
     1621 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaaact taatcgccag
     1681 ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaaat
     1741 gaccgccgca aatgagatcc gcgacatggc atcctgaaat gactcctgac tggtataggt
     1801 atggagctta taagggttac ccgcaaactt tctgaataaa ctctcaatac cgatactgct
     1861 catgacgcat ttttctatga gtgcgacatt tctcttctta attcggctgt gttccattct
     1921 gcggcatctc cataaacaga aggcaagtta agttctcttg caccctgcgc gctatttatc
     1981 catgcataca tttcagcgtt gctgtgaatt gccagtcgac gcatcgcact gtttttttgg
     2041 gcgctgatag ttttattact tttcttaagc aacgaagcta tctggttgat gccccatcct
     2101 ttacctaata gccgcagtac cttgcgctct gaaagagtga gaatgacgga gcgatagcta
     2161 aaatcatgag tttcattcat tgccggagag gtcagcatgg cgctgatacg ctctgcgtgc
     2221 gtattgccgg aacgtacggt cttgaccaga ttctcaatgg gttcaacatc agacaataac
     2281 gtcgccgtag ggcacatgag caattctact gcctgggaat accaggagcg tgacaccatg
     2341 aaaacccagt gaatttcccg atattgtgag attaaagaat aataatgttc acaaacagaa
     2401 cgtggatctt cactttcacc ggctaaatca gcaatgacta atccggaacg acgtaattgt
     2461 aataaggtaa ggtcctccgc agaggcgctg gatattattt catattcagg gaagtgagtc
     2521 ctcatgacct cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt
     2581 ttgcagcatc ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta
     2641 ttcaggatat tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat
     2701 ccttagcatt cactttcata tcagcatact cctgctgcac aacaactcca cagcatgtgt
     2761 cagaaggtta gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta
     2821 cattaagttg ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt
     2881 aaatgatatc ccttcccgca gaatgaaaaa tcacacaacc aatatttatt caatgaaatc
     2941 tgatggtttt aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt
     3001 aaaggccaaa caataaattc attatagtga taatattcat gtgatagttt gtttgcggcg
     3061 agagataatt cgctttttat caccgagctt gtcccgataa ccaggattaa tcagcgcaat
     3121 cttgatctta acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc
     3181 tgaacaatac gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag
     3241 ccgtaacacg gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg
     3301 cgttggttga tgagctttcc tcacgactgg gtcgggcgtt ggggatggac agcgtcgaaa
     3361 gttctatctc ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat
     3421 cgacacgtaa aaatcaagat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca
     3481 ttgtgattct ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc
     3541 aaattcagcc attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgtg
     3601 cctgtttctg taaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca
     3661 gtacggccgc gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccacaaatca
     3721 acttttgcct taccgctttc gccgccacca ccatttccgg attgcttttg caactcccca
     3781 ctttcagcaa tacccccacc attgcgatgg ccgccagcgt tctgctgctg gtgccgggct
     3841 ttccgttgat taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac
     3901 gctgggcgat cgccagtctg ctgacactgg ctacctgcgt cggcgtagtg atggcactga
     3961 cgatttgggg gctacgcgga tgggtgtgat cgaatttctg ttagcgttgg cgcaggatat
     4021 gatcctcgcc gccattcctg cggtcggctt tgcgatggtg ttcaacattc ccgttcgggc
     4081 gttacgctgg tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac
     4141 cagcgggctg aatattgagt ggtcaacttt tatggcttct atgctggtcg gcaccattgg
     4201 tattcaatgg tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat
     4261 ccctatgttc ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca
     4321 gttaggttac agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc
     4381 gattgttggt gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg
     4441 ccctcgcgta taaaattgcc tcgcttaagc acacggatga gagacagcct cctctcctcc
     4501 gtgtgttact ataaaagtta tctcccttct cgttcatcgt tccatatttg agaaatagta
     4561 tgtcttccag agttttgacc ccggacgtcg ttggtattga cgccctggta cacgatcacc
     4621 aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt atttgctaac aatgccccgg
     4681 cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa gaaaagctgg
     4741 cgcgtccggg aagcgatgtc gctctggacg atcaactcta tcaggaaccg caagccgctc
     4801 ccgttgctgt ccccatgggg aaattcgcca tgtatccgga ctggcaaccc gatgccgact
     4861 ttatccgcct ggcggcgcta tggggcgtgg cgctaagaga gccggtgacc accgaagaac
     4921 tggcctcatt cattgcctac tggcaggcgg aaggtaaagt ctttcaccat gtgcagtggc
     4981 aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga ctgccgaaac
     5041 gagatgtgaa tacggtcagc gaacctgaca gccaaattcc accaggattc agagggtaac
     5101 gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa
     5161 acctgcattc aaaacgggtg aagagctact ggcctggcag aaagaacaag gggcgatccg
     5221 ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc
     5281 cggtattcgt ccactgcatc agaactgctc ctttgagaac tatcgcgttg agtgtgaagg
     5341 gcagatgaat gcgttaagca aagcgcgcca gtatgtcgaa gagtttgacg gcaacatcgc
     5401 cagcttcatc ttttctggta agccaggaac cggcaaaaac catctggcgg cggcaatctg
     5461 caacgagctg ctgctacgcg gtaaatccgt attgatcatc accgtggccg atattatgtc
     5521 ggcgatgaaa gataccttca ggaatagcgg taccagtgaa gaacaactgc ttaacgatct
     5581 gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg cagaccgaat cgaaatacga
     5641 aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat
     5701 gctgaccaac agtaatatgg aagagatgac caagttgctg ggcgaacgcg tgatggaccg
     5761 tatgcgcctg ggtaacagtt tgtgggtgat cttcaactgg gatagctacc gtagccgggt
     5821 aacaggtaaa gagtattaat ccccgtttct ggcggtatac tgcgacacaa ttccaccttg
     5881 agtaatgatg atgaaaactg ttaaacatct tctgtgctgt gccattgccg ccagcgcatt
     5941 aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg ccgccagcga
     6001 acttggcaac caaaacaaca cgacacagga aggcggttgg tcgctcgcgt cattaactaa
     6061 cttgcttagc agcgggaacc aggccttaag cgcagataac atgaacaacg ccgcaggcat
     6121 tctgcaatac tgcgcgaagc aaaagctggc gtcggtaacc gatgccgaaa acatcaagaa
     6181 ccaggtgctg gaaaagctgg gcctgaacag tgaagagcaa aaagaagaca ccaactatct
     6241 ggatggcatt cagggtttgc tgaaaaccaa agatggtcag caactcaatc tggataacat
     6301 cggaacgact ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca
     6361 ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt ttaactgtta cccggctgac
     6421 gcggctctct cctgcccttt tccctgctcc tggacggttt tccccctgtt acacgggaag
     6481 cgaccgcgat caaaaaagtc tgtttataaa ctaattccta acaacatcgc atttttttga
     6541 cactagaatt gcagcaatat ggcgacgcca ttacattgtg cttctcaaaa agcttaaacg
     6601 ttggctaaac ggcccggttt agtgaggatc gcccgttgtc agaactactc tctttcgccc
     6661 tttttctcgc ctctgtgctg atttacgcat ggaaagcggg acgtaacacc tggtggtttg
     6721 cagccacatt aacggtgctg gggctatttg tcgttttaaa tatcaccctg tttgccagcg
     6781 actattttac tggcgatggt attaacgacg cggttctcta taccttaacc aacagcctga
     6841 ccggtgctgg cgtcagcaaa tacatcctgc cgggtatcgg cattgtgctg gggctgacag
     6901 cggtgttcgg tgcgctgggc tggatcctgc gccgtcgtcg ccatcatccg caccattttg
     6961 gttacagcct gctggcgctc ttactggcgc tgggttcggt ggacgccagc ccggcatttc
     7021 gtcagataac ggaactggtg aaatcccagt cacgcgatgg cgacccggac tttgcggctt
     7081 attataaaga gccgtcgaaa actatccctg acccgaaact caacctggtt tatatctacg
     7141 gcgaaagtct cgagcggacc tattttgata acgaagcttt cccggatctc acgcctgaac
     7201 tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac gcagcagctg ccaggaacgg
     7261 attacacgat tgcgggcatg gtggcttctc agtgcggcat accgctgttt gccccctttg
     7321 aaggcaacgc ctccgcctct gtctccagct tcttcccgca gaacatctgt ctgggcgata
     7381 tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg cgcgaatctg cgttttgccg
     7441 gtaaagatgt gttcctgaag tcgcatggct tcgaccactt atacggctca gaagagctga
     7501 aaagcgtggt ggccgacccg cactatcgca acgactgggg attctacgac gataccgttc
     7561 tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc aggtcagcga ttctcactgt
     7621 ttaccctgac ggtcgatacc catcacccgg atggttttat ctcgcgcacc tgtaaccgca
     7681 aaaaatatga ttttgacggt aagcctaatc agtcattcag cgcggtaagt tgcagccagg
     7741 agaacatcgc gacgtttatc aacaaaatca aagcgtcacc gtggtttaaa gataccgtca
     7801 tcgtcgtctc ttctgaccat ttagcgatga acaacacggc gtggaaatac ctcaataagc
     7861 aggatcgcaa taacctgttt tttgtcattc gtggcgacaa gccgcagcaa gagacgctgg
     7921 cagtgaagcg taacacgatg gataacggcg cgacggtgct ggacattctc ggtggcgata
     7981 actatctcgg acttggtcgt agcagtttat ccgggcagtc gatgtcggaa atcttcctca
     8041 atatcaaaga gaaaacattg gcgtggaagc cagatatcat ccgcctgtgg aaattcccta
     8101 aagagatgaa agagttcacc atcgaccagc agaaaaacat gattgccttc tcgggtagcc
     8161 atttccgttt gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg ctgccggaaa
     8221 gcgaatactc agcaccgctg cgtttccagc tggccgattt cgctccacgc gacaatttcg
     8281 tctgggttga ccgttgctac aagatggcac aactctgggc tccggaactg gcactttcca
     8341 ccgactggtg tgtctcgcag gggcagcttg gcggtcagca aattgttcag catgttgaca
     8401 aaacaacatg gaagggcaaa acggcattta aagatacggt catcgacatg gcgcgttaca
     8461 aaggcaatgt cgatacgctg aagattgttg ataacgatat tcgctacaaa gccgacagtt
     8521 tcatctttaa cgtcgccggt gcgccagaag aggtgaaaca gtttagcggg atttcccgtc
     8581 cggagtcgtg gggccgctgg tccaacgcgc agctgggcga tgaagtaaaa atcgagtaca
     8641 agcatccgct gccgaagaaa tttgacctgg tgattaccgc caaagcatac ggcaataacg
     8701 ccagccgtcc tattccggta cgcgtaggca atgaagaaca aactcttgtg ctgggcaatg
     8761 aagtgaccac caccacactg catttcgata acccaaccga tgccgacaca ctggtaattg
     8821 tgccgccgga acctgtctca accaacgaag ggaatatcct cggacactcg ccgcgtaagc
     8881 tcgggatcgg catggtggaa attaaagtgg tagaacgtga agggtaattc ctgactgatg
     8941 tgtaggtcgc ataaggtgcg acctacacac cttctacggc ttacaaaaca tcattgagaa
     9001 acagaaaaaa acagcgccca acacttcccc ggacgctgca tggcaacttt aataatctcg
     9061 ccttatcaga aagtaattac acccttaatc aaatcacgat tgttaatcac atcgcgctcg
     9121 taggtttctg ccagcgtggc gaacggatag cgatgggtta acatcatgtc agcagtgatt
     9181 ttcccttccg ccatcagtcg accgacttta gcgaaatctt ccggcgtggc gttgcggctg
     9241 cccatcatcg tcgtttcttt tttatggaat tccagatcgg agaactgcaa ctcaccttta
     9301 aacaggccga caaataccac cgtgccgccg tgacgaatca gattcacggt gttattcatc
     9361 gcatgttgat tacctgtcgc gtcgatcact ttctgcgcca gcgaaccacc aaactgcgcc
     9421 cgcagctgcg cgtcaaaatc ttcggctgac gggtccagca cgggcaactc cagacgcgtt
     9481 gccacatgtt cacggcgcgc cggactggta tccgccacca ccacctgtgc gccatcggct
     9541 ttagcgattg ccgccgcgcc cagaccgatt ggccccgccc cgaccaccag cacctgctcg
     9601 ccgggagcaa tggctgcgcg acgcaccgca tgagcgctaa tagcgaaagg ttcaatcaat
     9661 gctgccgcct gcgggtcaat accgtctgcg ggcaaaatgt tcgccaccgg cacgctcaaa
     9721 tactcactaa aaccgccatc ctgatgcacg ccaatgactg aaattttttc acagcaattg
     9781 gtacgcccgc ttttacacgc cgggcattgc tgacaggcaa cataagggat cacagcaact
     9841 tgctgaccat ttttaagatc agcaatattt ttacccagcc caacaatctc cccacatatt
     9901 tcatggccta aaacacgtgg ataactaaaa aatggttgat ttccacccca ggcatgaata
     9961 tcggtaccgc aaatcccgac agacttaatt tttattaatg cttcattgtc acccggaata
    10021 ggtatctcgc gttgtttcca gactaattct ttcggctgct ggcaaattaa aacattcatc
    10081 gtagacatga ttcggctctc cattttttgt tgccttagat atcaatgaaa atgaaaaatg
    10141 gcagcggaac ggaaaatctg ttttgtgaaa acacacgcat aaaaatgttt taaatcgggt
    10201 tttaatggac tcacaaggag gaaaatatga gtcgttcaca aaatttacgc cacaatgtga
    10261 ttaaccaggt cattgatgat atggcgcggg gtcatatccc ttccccactt ccatcgcaaa
    10321 gtgcgctggc ggaaatgtac aacatcagcc gcaccacagt gcgtcatatt ctcagccact
    10381 tacgcgaatg cggcgtcttg acgcaggttg gaaacgacta tgttattgcg cgtaaacctg
    10441 accatgatga cggtttcgcc tgtactaccg cttcaatgag tgaacaaaac aaagtgtttg
    10501 agcaggcttt tttcacgatg ataaaccagc gccagttacg cccaggggaa acgttttctg
    10561 aactgcaact ggctcgggcc gcgggtgtca gcccggttgt agtaagagaa tatcttttaa
    10621 aattcggccg ttataatctt attcacagcg aaaagcgcgg ccagtggagc atgaagcaat
    10681 tcgatcaatc ctacgccgag cagctgtttg agctacgcga aatgctcgaa acacattcac
    10741 ttcagcactt tctcaacctg cctgatcatg atccccgctg gctgcaggcg aaaaccatgc
    10801 tcgaacgtca tcggctactg cgtgacaaca tcggcaatag ctttcgcatg ttctcgcaac
    10861 ttgacagaga ttttcactcc ttactgcttt cagctgccga caatatcttt tttgatcaat
    10921 cgcttgagat catctcagtc atctttcatt ttcactatca atgggacgaa agcgatctta
    10981 aacagcgcaa tatcattgcc gtcgatgagc atatgaccat tctcagcgct ctgatttgcc
    11041 gcagtgacct tgatgccacc cttgcgctcc gtaaccattt gaattcagct aaacaatcaa
    11101 tgattcgatc cattaatgag aacacgcgtt atgctcatta agtacggatt aaaaacagca
    11161 aactattagc gggtgcacgg ttttatcaaa agacaaatac cgggaaattc ccgcttacct
    11221 atgctcacaa tcagtcgact aaaaatgttt ttgtgagcgg tagtaaagtc ctaaaacttt
    11281 aacctgccgt taatgtcaac cttgcgtaat tcacaattac gtaaacaatt aaaattataa
    11341 atacaggagt ccggcgtgga aaaagaaaat atcaccctcg atccgcgttc ttcatttact
    11401 ccatcttcgt cggcagatat tcccgtgcca ccagatggat tagttcaacg cagtacccga
    11461 attaaacgca ttcaaaccac cgccatgttg ttattatttt ttgcggcggt aatcaattat
    11521 ctcgaccgca gttcgctgtc ggtagcaaat ttaacgattc gtgaagaatt gggattaagt
    11581 gccaccgaaa tcggcgcttt gctctccgtg ttttcactcg cttacggcat tgcgcaactt
    11641 ccttgcggcc cactattgga tcgtaaaggc ccacgcctga tgctgggact ggggatgttc
    11701 ttctggtcac tgttccaggc catgtctggc atggtgcaca gctttacgca gttcgtgttg
    11761 gtgcgtatag gtatggggat tggtgaagcg ccgatgaacc catgcggtgt aaaagtcatt
    11821 aacgactggt tcaacatcaa agagcgcgga cgcccgatgg gcttcttcaa cgcagcttct
    11881 accattggcg ttgccgtaag cccaccgatt ctggcggcga tgatgctggt gatgggctgg
    11941 cgcgggatgt ttattaccat tggtgtactg gggatttttc tcgccatcgg ctggtatatg
    12001 ctctatcgca accgcgagca cgtagaactg actgccgttg aacaagctta tctcaatgca
    12061 ggtagcgtca atgcccgccg agatccgctc agttttgccg aatggcgcag cctgttccat
    12121 aaccgtacaa tgtggggaat gatgctcgga ttcagtggca tcaactacac tgcgtggctg
    12181 tatctggcct ggcttcctgg ttacctgcaa acagcctata acctggattt aaaaagcaca
    12241 gggttgatgg cggctatccc tttcctgttt ggggctgccg ggatgctggt caacggttac
    12301 gttactgact ggctggtcaa agggggaatg gctccgatta aaagccgtaa gatctgcatt
    12361 attgccggga tgttctgttc tgccgccttt acgctgatag taccacaagc gacaacatcc
    12421 atgacggcgg ttctgctgat tggcatggca ctgttctgta ttcactttgc cggaacatcc
    12481 tgctggggct tgatccacgt cgcagttgct tctcgcatga ctgcgtcggt gggcagtatc
    12541 cagaactttg ccagcttcat ctgcgcctct tttgcgccga tcattactgg ttttattgtt
    12601 gataccaccc actcattccg tctggcacta atcatctgcg gttgcgtcac cgcagcgggg
    12661 gcactggcgt acatcttcct ggttcgtcag ccgatcaacg acccacgtaa agattaatct
    12721 ccttatgccc gataacattt tgcttatcgg gcattttcat ggcgattaaa atgtttccca
    12781 gttctcctcg ctatctgcca cggccatttt acgcggcgca gctggcgtca cggtttttac
    12841 cacagccgat gtttcacgct gctgttgctg ctgttgctga atccggaaca ctgccacagc
    12901 ttcggtcagg cgactggcct gctcttccag cgcggcgttc tgttgagtta cccggtccat
    12961 ctcagcaacc gctaagccaa cctgatcgat accacggctc tgctcatcag aagcagaagc
    13021 aatttcgccc ataatgtccg tcacgcgggt cacggcgctg acaatctccg ccattgtttc
    13081 cccggcgctt tcgaccagcg tagagccaac atccactttc cccaccgagt cttcaatcag
    13141 gcttttaatt tcacgagccg cctgggcgct gcgctgggcc agattacgca cttctcccgc
    13201 gaccaccgca aaaccgcgcc cttgctcacc cgcacgcgca gcctcaaccg ccgcgttcaa
    13261 agccagaata ttggtctgga aggcaatgcc gtcaattacg ctgataatat cggcgatttt
    13321 ctgcgaactg gtggagatat cgcgcatagt ctgcaccacg ttatctacca ctttgccgcc
    13381 gcgttgcgcc gtttcagaag cacttaacgc cagatggctg gcctggcgcg cattttcggc
    13441 gttctgcttc accgttgcgg tcagttgctc catgctggct gccgtctctt ccagcgaagc
    13501 ggcctgttgc tcggtgcgcg aagagagatc gttattgccg gtggcgattt cgctggcacc
    13561 gctatagatg gcattggccc cgttgcgcac atcaccgacg gtacgcatca gctctccctg
    13621 catatggcgc aaactctctg ccagttgccc catctcatta gagccatcca cctcaatcgg
    13681 tttcaccaga tcgccgcctg caatatgacg aatgctgtca atcaggcgat tcattggcgc
    13741 taccagcgag gctttaatac cgaaccagac ggcgaagatg accgccagta cgacgatcat
    13801 cacgcccacc agaatccaca tcgcctggct gtaggaggca ttgttatcgc tgacggcgat
    13861 atcatagagc cgatcgtttt gctccatgta agccacatac tgcttctcga aaccgtcctg
    13921 atatccctgg gtcggctgat caaagaactc gttgattttg cctgcaccta acagttggat
    13981 cagctccgcc agcgcattgt gataaatatc gtaattacgt ttgatctctg ccgctgcggc
    14041 ggtgctctga cgcgggtcac gcggcaacgc ttcgtaatcc gcccagtttt tttccgcctg
    14101 tttcagcgaa atactggcac tctgcatcag ctcagcaacg gttgaaccgc taccaatatt
    14161 attctgatcc atcatgtagc ggatacccgc gcggttgagg gtgttacgcg tctgcaacaa
    14221 cgcgacccag ctgccattca gcgtggattg ctgctggcga atggtttgta aaacagtgaa
    14281 attttctttg tcattcttta aggcattaaa gaacagaccg cctgatgtca gttgtaaaag
    14341 gccaaaaacg gccaaaacca gcagtaagct ggtcacgatc ttgatacgtt ttaacatggt
    14401 ttctctttcc tgtggaccga tgcaagattt tcggcctgga aagcaaaaac tttatgaaaa
    14461 ggtgtaaccc gccatccatg gaaaaaccag gcgctggtta tcagatacta aaaagttatt
    14521 cattatcgcc cgggatggct tcttgtcggg aattgcccag agcaataaat tcctctaaca
    14581 gatgggttaa ctgttgcatt ttctcggcag taaactgagc ttcaatctgc cgataggctt
    14641 cttcaatctg tgtctgagcg cggttataca gcgcctgccc ctctttggtt agcgagatat
    14701 agagcttccg ctgatcattg atcggcttta atcgcaacac taaaccgtcg cgttccatcc
    14761 gcgtgaggat cccggtcagg cttgggcgca aaatgcaggc gcgatacgct agatcgtgaa
    14821 aatccattga tgggctttcc gccagaatac gcacgatgcg ccactgttgc tcagtcaaat
    14881 tatgccgttt aacgattggg cgaaaataac tcatcgccgc ttctcgcgcc tggagcaacg
    14941 caatggttag tgagtcgtgc attatcttcc cc
//
LOCUS       DAZKHC020000099        14957 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZKHC020000099 DAZKHC020000000
VERSION     DAZKHC020000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.099"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..458
                     /locus_tag="ACHOSM_RS18620"
                     /old_locus_tag="pgaptmp_003724"
     CDS             <1..458
                     /locus_tag="ACHOSM_RS18620"
                     /old_locus_tag="pgaptmp_003724"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGU4560149.1"
                     /translation="MGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPL
                     DNALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTI
                     VELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF"
     gene            517..720
                     /locus_tag="ACHOSM_RS18625"
                     /old_locus_tag="pgaptmp_003725"
     CDS             517..720
                     /locus_tag="ACHOSM_RS18625"
                     /old_locus_tag="pgaptmp_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGU4560150.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            720..1770
                     /locus_tag="ACHOSM_RS18630"
                     /old_locus_tag="pgaptmp_003726"
                     /pseudo
     CDS             720..1770
                     /locus_tag="ACHOSM_RS18630"
                     /old_locus_tag="pgaptmp_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1798..1887)
                     /locus_tag="ACHOSM_RS18635"
                     /old_locus_tag="pgaptmp_003727"
     tRNA            complement(1798..1887)
                     /locus_tag="ACHOSM_RS18635"
                     /old_locus_tag="pgaptmp_003727"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1851..1853),aa:Ser,seq:cga)
     gene            1981..2778
                     /gene="mtfA"
                     /locus_tag="ACHOSM_RS18640"
                     /old_locus_tag="pgaptmp_003728"
     CDS             1981..2778
                     /gene="mtfA"
                     /locus_tag="ACHOSM_RS18640"
                     /old_locus_tag="pgaptmp_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HGU4560151.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            2879..2954
                     /locus_tag="ACHOSM_RS18645"
                     /old_locus_tag="pgaptmp_003729"
     tRNA            2879..2954
                     /locus_tag="ACHOSM_RS18645"
                     /old_locus_tag="pgaptmp_003729"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2912..2914,aa:Asn,seq:gtt)
     gene            3268..10343
                     /gene="yeeJ"
                     /locus_tag="ACHOSM_RS18650"
                     /old_locus_tag="pgaptmp_003730"
                     /pseudo
     CDS             3268..10343
                     /gene="yeeJ"
                     /locus_tag="ACHOSM_RS18650"
                     /old_locus_tag="pgaptmp_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(10605..11657)
                     /locus_tag="ACHOSM_RS18655"
                     /old_locus_tag="pgaptmp_003731"
     CDS             complement(10605..11657)
                     /locus_tag="ACHOSM_RS18655"
                     /old_locus_tag="pgaptmp_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HGU4560152.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEKTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            11972..13288
                     /gene="shiA"
                     /locus_tag="ACHOSM_RS18660"
                     /old_locus_tag="pgaptmp_003732"
                     /pseudo
     CDS             11972..13288
                     /gene="shiA"
                     /locus_tag="ACHOSM_RS18660"
                     /old_locus_tag="pgaptmp_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
     gene            13390..14844
                     /gene="amn"
                     /locus_tag="ACHOSM_RS18665"
                     /old_locus_tag="pgaptmp_003733"
     CDS             13390..14844
                     /gene="amn"
                     /locus_tag="ACHOSM_RS18665"
                     /old_locus_tag="pgaptmp_003733"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HGU4560153.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         4077 a         3558 c         3559 g         3763 t
ORIGIN      
        1 atatgggacc ggaatttagt aagactatcg atctggaaac tgctggcgga gtcattgatc
       61 gggacaccat taaatggtgg cttaagcaat cacgcgaagc gcaatctgcc attatgaccg
      121 atgaaatccc gttagataat gcactgttac aattgcggga atttatcgac gaaaactccg
      181 gtgaattttt tgttcaggtc tggggaaatg gagccaactt cgacaacacg attttgcgcc
      241 gttcatacga acggcagggg atcccctgcc cgtggcgtta ctacaacgat cgcgatgtac
      301 gcacaatcgt tgagctgggg aaagccatag acttcgatgc cagaactgct atcccattcg
      361 aaggtgagcg ccataatgca cttgatgacg ctcgttacca ggcaaaatac gtttcagcta
      421 tctggcaaaa actgatcccg agtcaggctg atttttaatg ttcaacccta attgccgcta
      481 accgtatata gttagcggcg gttatgagat atggctatga gcagcttatt tttaaccgaa
      541 gatgaattgc taatattaac aggctgcaaa tatgcaagcc accagcgaaa atggttaatg
      601 gaaaacgggc ttccgttcta taccaatcgt agtggcaaac cgattgtcag ccgggatcta
      661 tttacctgca ataaaacttt accaccacgc gaggtagagc cgaattttgg tgcaatctga
      721 tgggaagacg aaggaaaaat cctgaacacg aaaaattacc tccaaatgta tacccaaata
      781 aatatagtta tgtatggaaa ccaacatcca gagaatctgt aacactaacc gccatcaagg
      841 atggtttagc tgctttatgg aaaaagtatg aggaaactgt aaataatcac gatcgtgcaa
      901 tgacattcgg tcgcttgtgg gaaaaattcc tcgccagcgc ctattacagt gaccttagtc
      961 caagaacaca aaaagattat ctgcaacatc aaaaaaagtt gcttgccgta ttcggtaagg
     1021 taccagcgga tgccataaaa ccagaacaca tccgtcgata catggacaaa agaggggagc
     1081 agagtaaaac gcaagccaac catgaaaaaa gcaatatgtc ccgcgtttac agttgggggt
     1141 atgagcgagg ttacgtgaag ggtaacccat gtgcaggtgt aagtaaattc aaggccaaaa
     1201 accgcgaacg atatgtaacc gacaaagaat accaggcagt attaagcgtt gcacctcttc
     1261 ctgtttttat cgcaatggaa attgcctatc tgtgtgcagc gagggtttcc gatgtgttat
     1321 cactgaaatg ggagcagatt ggaaacgacg ggatctttat ccagcaaggg aaaacaggga
     1381 aaaaacagat aaaagcatgg agtccacgat tacaggcggc gatcgaaaaa gcaaaacagt
     1441 taccagcatc cgtctatgta atcagcaatc aatatggcaa ccgatatatg tacaaaggct
     1501 ttaacgaaat gtgggtagaa gcaagaaatc gcgcaggcaa aatttcaggt attttaaccg
     1561 acttcacctt tcatgatctg aaggcgaaag gaatttcaga ctatgaagga agcagccggg
     1621 ataagcaact tttctctggt cacaaaaccg aggggcaagt gctaatctat gacaggaagg
     1681 ttaaagtttc accaacactt gatgtcccgt tacctgaaaa tattccaaga aaatattcca
     1741 agtaattcca agtgtgattt ttgtcactga cttaatgatg tgtaagtgat tgaattttgg
     1801 cggagagagg gggatttgaa cccccggtgg agttgccccc actccggttt tcgagaccgg
     1861 tccgttcagc cgctccggca tctctccgtt cagatggttg ccatgatgcc aggaaatttg
     1921 gcattttaac agtccctgtc cgtgcaattt tgttcaagtg acgagtttgc gagcaaaacg
     1981 atgattaagt ggccctggaa agtacaagaa tcagcacatc aaactgccct tccctggcag
     2041 gaagcactat cgatccccct tttaacgggt ctgacggaac aggaacaaag caaattagtc
     2101 actcttgccg aacgtttttt acagcaaaag cggcttgttc ctttacaggg ctttgaactg
     2161 gattcattaa gaagctgccg gatagcactt ctattttgcc tacccgttct ggagttagga
     2221 ctggaatggc tggatggttt tcatgaagtc ttaatttatc ctgcgccatt tgtggtcgat
     2281 gatgaatggg aagacgatat cggtctggtg cataaccaac gtattgttca gtcaggtcag
     2341 agctggcagc aagggcctat cgttttgaac tggttggata tacaagattc ttttgatgct
     2401 tctggtttta acctgattat tcatgaagtc gctcataagc tggacacccg taacggcgat
     2461 cgcgccagcg gagttccctt tattccgttg cgtgaggttg ctggctggga acacgatctt
     2521 catgctgcaa tgaacaacat tcaggaagaa atcgaattgg ttggcgagaa tgcggcgagc
     2581 attgatgctt atgctgccag tgatcctgct gaatgttttg ccgtactttc tgaatatttc
     2641 tttagcgccc cagaactttt tgctcctcgt ttcccttcat tgtggcaacg tttctgccaa
     2701 ttttatcaac aagatccttt gcagagactg catcgcacta atgatacaga ctcgttttcg
     2761 gcgacgaatg ttcattaatt aacgactttg cagattaatt aaccaattga aatgacttat
     2821 gaaatttagt gttgacagac aaggtaccgc taagtaatat gcgccccgtt cacacgattc
     2881 ctctgtagtt cagtcggtag aacggcggac tgttaatccg tatgtcactg gttcgagtcc
     2941 agtcagagga gccaaattct aaaaattcgc ttttttagcg caatgtcact gaccttagtt
     3001 gaacattgtt ttttaacgga tagcgggttt ttaacatctt aagcgccctc gacctttatg
     3061 gttgagggcg ttttgctatg aacgccatca ccattttccc ctcgattata aaacttgagt
     3121 tattcagtag tctcccctct tgcaactcac acccaaaact gcctaacgaa aagttattaa
     3181 ttttcaatca tattgctatc aggatttaca ttttttcgct gtgctagaaa gggcgcattt
     3241 atgttagctc gttcagggaa ggtaagcatg gctacgaaga agagaagtgg agaagaaata
     3301 aatgaccgac aaatcttatg cgggatggga attaaactac gccgcttaac tgcgggtatc
     3361 tgcctgataa ctcaacttgc gttccctatg gctgcggcag cacaaggtgt ggtaaacgcc
     3421 gcaacccaac aaccagttcc tgcacaaatt gccattgcaa atgccaatac ggtgccctac
     3481 acccttggag cgctggaatc ggcccaaagc gttgccgaac gtttcggtat ttcggtggct
     3541 gagttacgca aactcaacca gtttcgtacg tttgctcgag gttttgataa tgtccgccag
     3601 ggtgatgaac tggatgtccc ggcacaagtt agtgaaaaaa attaaccccg ccgccgggta
     3661 atagcagtga caacctcgag caacagatag ccagtacttc acagcaaatc ggggctctgc
     3721 tcgccgaaga tatgaacagc gagcaagcgg caaatatggc gcgtggatgg gcctcttctc
     3781 aggcttcagg cgcaatgaca gactggttaa gccgcttcgg taccgcaaga atcacgctgg
     3841 gcgtggatga agattttagc ctgaagaact cccagttcga ttttctccat ccgtggtatg
     3901 aaacgcctga taatctcttt ttcagtcagc atactctcca tcgtactgac gagcgtacgc
     3961 agattaacaa cggcttaggt tggcgtcatt tcactcccac atggatgtca ggcatcaact
     4021 tctttttcga ccacgatctt agccgttacc actcccgcgc cggcattggc gcggagtact
     4081 ggcgcgacta tctaaaatta agcagtaacg gctatttgcg actgaccaac tggcgcagcg
     4141 cacctgaact ggacaacgat tatgaagccc gcccggccaa tggctgggat gtacgcgcag
     4201 aaggctggct acccgcctgg ccgtaccttg gcggtaaact ggtctatgaa cagtattatg
     4261 gcgatgaagt ggccctgttc gataaagacg atcggcaaag taatcctcat gccataaccg
     4321 ctggacttaa ctataccccc ttcccgctga tgaccttcag cgcggagcaa cgccagggta
     4381 aacagggcga aaatgacacc cgttttgccg tcgattttac ctggcaacct ggcagcgcaa
     4441 tgcagaaaca gcttgacccg aatgaagtcg ctgcacggcg tagccttgca ggcagccgtt
     4501 atgatctggt ggatcgcaac aacaatatcg ttctggaata tcgcaaaaaa gaactggttc
     4561 gcctgcccct gacagacccc gtgacaggga agtcaggaga agtgaaatca ctggtttcgt
     4621 cgctacaaac caaatatgcc ctgaaaggct ataacgtcga agccaccgca ctggaagctg
     4681 ccggtggcaa agtggtcaca acgggtaaag atattctggt taccctgccg gcttaccggt
     4741 tcaccagtac gccagaaacc gataacacct ggccgattga agtcaccgcc gaagatgtca
     4801 aaggcaattt gtcgaatcgt gaacagagca tggtggtcgt tcaggcacct acgctaagcc
     4861 agaaagattc ctcggtatcg ttaagtaccc aaacattgaa cgcggattcc cattcaaccg
     4921 ccacactgac ttttattgcg catgatgcag caggtaatcc tgttgtcggg ctggtgctct
     4981 cgacgcgtca cgaaggtgtt caggacatca ccctttctga ctggaaagat aatggtgacg
     5041 gaagctatac ccagatcctg accacaggtg cgatgtctgg cacgctgacg cttatgccac
     5101 agctgaacgg tgtggatgcg gctaaagccc ccgccgtggt gaatatcatt tctgtttcgt
     5161 catcccgaac tcactcgtca attaagattg ataaggaccg ttatctctcc ggcaatccta
     5221 tcgaggtgac ggtagaactg agagatgaaa atgacaaacc tgttaaggaa caaaaacagc
     5281 aactgaataa cgcagtcagc atcgacaacg tgaaaccagg agtcactaca gactggaaag
     5341 aaaccgcaga tggcgtctat aaggcgacct ataccgccta taccaaaggc agtggactta
     5401 ctgcgaagct attaatgcaa aactggaatg aagatttgca taccgctgga tttatcatcg
     5461 acgccaaccc gcagtcagcg aaaattgcga cattatctgc cagcaataat ggtgtgctcg
     5521 ccaatgagaa tgcagcaaac accgtctcgg tcaatgtcgc tgatgaagga agcaacccaa
     5581 tcaatgatca taccgtcacg tttgcggtat taagcggatc ggcaacttcc ttcaacaatc
     5641 aaaacaccgc aaaaacggat gttaatggtc tggcgacttt tgatctgaaa agtagtaagc
     5701 aggaagacaa cacggttgaa gtcacccttg aaaatggcgt gaaacaaacg ttaatcatca
     5761 gttttgtcgg cgactcgagt accgcgcagg ttgatctgca gaagtcgaaa aatgaagtgg
     5821 ttgctgacgg caatgacagc gccacaatga ccgcgaccgt ccgggatgca aaaggcaacc
     5881 tgctcaatga cgtcatggtc actttcaatg ttaattcagc agaggcgaaa ctgagccaaa
     5941 ccgaagtgaa tagccacgac gggatcgcca cagctacgct gaccagtttg aaaaatggtg
     6001 attatagggt tacggcctct gtgagctctg gttcccaggc taatcaacag gtgaatttta
     6061 tcggtgatca aagtactgct gccctgaccc tcagtgtgcc ttcaggtgat atcaccgtca
     6121 ccaacacagc tccgcaatat atgactgcaa ccttgcagga taaaaatggc aacccactaa
     6181 aagataaaga aatcaccttc tctgtgccaa acgacgtcgc aagtaagttc tcgattagca
     6241 acggaggaaa aggcatgacg gatagtaacg gggttgcaat cgcctccctg accggcacgt
     6301 tagcgggcac gcatatgatc atggctcgtc tggctaacag caatgtcagc gatgcacagc
     6361 caatgacgtt tgtggcggat aaagacagag cggttgtcgt tttgcaaaca tcgaaagcgg
     6421 aaatcattgg gaatggcgtg gatgagacaa ctctgacagc aacagtgaaa gatccgtcga
     6481 atcatccggt ggcggggata acggtaaact tcaccatgcc acaggacgtt gcggcaaact
     6541 ttacccttga aaataacggt attgccatca ctcaggccaa tggggaagcg catgtcacgc
     6601 tgaaaggtaa aaaagcgggc acgcatacgg ttaccgcaac gctgggtaat aacaatacca
     6661 gtgattcgca gccagtaacg tttgtggcgg acaaaacctc ggctcaggtt gtcctgcaga
     6721 tgtcaaaaga tgagatcaca ggtaatggcg tcgataacgc aacgctaact gcaacggtta
     6781 aagatcagtt cgacaatgag gtgaataatc ttccggtaac attcagctca gcctcttcag
     6841 gactcaccct gaccccggga gtaagtaata ccaatgagtc tggcatcgcg caggccactc
     6901 tcgcaggcgt tgcctttggt gagcagacgg tcactgcatc actggctaat aatggtgcca
     6961 gcgacaacaa aactgtgcat tttattggcg acacagcggc ggcaaaaatt atcgagttga
     7021 cgcctgtccc agacagcata atcgccggta ccccgcagaa cagctccggc agcgtcatca
     7081 ccgccacagt cgttgataat aatggctttc cggtgaaagg tgtgactgtg aacttcacca
     7141 gcagaacaaa ctctgccgaa atgacgaatg gcggccaagc cgtaacgaac gaacagggta
     7201 aggctaccgt cacttatacc aatacccgct cctcgataga atcaggagcg agaccggata
     7261 ccgttgaggc cagtctggaa aatggtagct ccacgcttag cacatcaatt aatgtcaacg
     7321 ctgatgcgtc tacggcacat ctcaccttgc tacaggcact ttttgataca gtctccgcag
     7381 gcgagacaac cagtctgtat attgaggtga aggataatta cggcaacggt gtcccccagc
     7441 atcaggtgac cctcagcgtt tcaccaagtg aaggcgtgac cctcagtaat aacggtatat
     7501 atactaccaa ctactacggc aatttttacg caagctttac cgctacaaaa gccggggttt
     7561 atcaagtgac ggcaaccctc gaaaatggtg attcgatgca acaaacagtg acctatgtgc
     7621 cgaacgtcac gaatgctgaa atcacgctgg cagcctcgaa ggatccggtg attgccgaca
     7681 ataacgatct cacgacacta acagcaacag tcgctgatac agaaggcaat gcgatagcca
     7741 gcactgaggt aacatttact ctgccggaag atgtgaaggc gaacttcacg cttagcgatg
     7801 gcggtaaagc gattactgat gctgatggca aagcgaaagt cacgctgaaa ggtacaaaag
     7861 caggcgctca tactgttata gcatcgatga ctggcggtaa gagtgagcag ttggtggtga
     7921 actttattgc ggatacgctc actgcgcagg ttaatcttaa cgttaccgag gacaacttta
     7981 tcgccaataa cgttgggatg accacacttc aggcaacagt gactgatgga aacggcaacc
     8041 cgttagccaa tgaggcggtg acattcacgc taccggcaga tgtaagcgca agctttactc
     8101 tcggacaagg cggttccgcc attactgaca tcaacggcaa ggctgaagtt acactgagcg
     8161 gtacaaaatc cggcacctac cccgtgacag ttagcgtgaa caattatggt gtcagtgata
     8221 cgaaacaggt gacgttgatt gccgatgctg gtaccgcaaa actagcctcc ttaacctctg
     8281 tatactcatt cgtcgtcagc acgaccgagg gcgcgaccat gactgcaagc gtcactgacg
     8341 ctaacggcaa cccggtagaa ggcataaaag ttaatttccg cggaacctcc gtcacgctaa
     8401 gcagcaccag cgttgaaacg gatgatcggg gtttcgctga aattcttgtg acaagcaccg
     8461 aggtcggact gaaaacagtt tcagcctctc tggcagataa acctactgaa gtcatctcgc
     8521 gattactgaa tgccagtgca gatgttaatt ctgcgacgat taccagtctg gagatacctg
     8581 aaggtcagct aatggtcgca caagacgtag cagttaaagc tcacgtcaac gaccagtttg
     8641 gcaacccgat tcttaatgaa tctgtaacat tcagtgcaga gccaccagag cacatgacca
     8701 tcagccaaaa tattgtctct actgatacgc atggtatagc cgaggtctcc atgacgcccg
     8761 aaagaaacgg ttcgtatatg gtgaaagcat ccctggcgaa tggagcctca cttgagaaac
     8821 aactggaggc tattgatgaa aaactgacac tcacggcgtc cagtccgctt atcggtgtct
     8881 atgcccctac aggcactact ctgacggcaa cgctaacctc tgcaaatggc actccagtgg
     8941 agggtcaggt catcaacttt agcgtaacgc cagaaggggc gacgttaagt ggcggaaaag
     9001 tgagaactaa ctcttcaggt caggctccgg tcgttctgac cagcaataaa gtcggtacat
     9061 atacggtgac tgcatcgttc cataacggcg taacaataca gacacagaca accgtgaaag
     9121 tcactggcaa ctcaagcacc gcacatgttg ctagctttat cgctgatcca tcgactatcg
     9181 ccgccaccaa cagtgattta agtaccttaa aggcaacggt tgaggatggc agtggtaacc
     9241 tgatcgaagg tctcactgtg tacttcgcct taaaaagcgg ctctgccaca ttaacgtcat
     9301 taacagcggt gaccgatcaa aacggaatcg cgacaacaag cgtgaaagga gcgatgacag
     9361 gtagcgtcac ggtaagcgca gtcacgaccg ctggtggaat gcaaacagta gatataacgc
     9421 tggtggcagg cccggcagac gcctcgcagt ccgtccttaa gaacaatcgg tcctcactga
     9481 aaggggactt taccgatagt gctgagctac atcttgtttt gcacgatata tcaggcaatc
     9541 cgatcaaagt ttctgaaggg atggaatttg tgcaatcagg tactaacgtg ccctatatga
     9601 aaattagcgc aattgattac agtcaaaata tcaacggtga ttacaaagcc accattacag
     9661 gcggcggaga gggtatcgca acgctgctcc ctgtattgaa tggtgttcat caagctggtc
     9721 tgagtaccac aatacaattc actcgcgcag aagacaaaat aatgagcggt acagtatcag
     9781 tcaatggtac tgacctaccg acaactacat tcccttcgca ggggttcacc ggggcgtatt
     9841 atcagttgaa taatgacaac tttgccccag gaaaaacggc ggctgattat gagttttcaa
     9901 gctctgcctc ctgggtcgat gttgatgcta ccggtaaagt gacatttaaa aatgtcggca
     9961 gcaattggga aaggactacg gcgacgccaa aatcaggagg ccctagctat gtatacgaaa
    10021 tccgtgtgaa gagttggtgg gtgaactccg gcgatgcttt catgatatac agccttgctg
    10081 aaaatttttg cagtagcaat ggctacacac ttccccgtgc agaccattta aaccatagtc
    10141 gttcccgagg catcgggtca ctgtacagtg aatggggaga tatggggcat tacacgactg
    10201 aagctggttt tcaatcaaat atgtattggt catctagtcc cgcaaactca agcgaacaat
    10261 acgtagtttc cctggcaaca ggtgatcaaa gcgtatttga aaagcttggg tttgcttatg
    10321 cgacatgtta taaaaacctg tgattttccg ttataatttc ttaaagagtg ctccattcgg
    10381 agcactcttt tttacattcc ctctatagag cttgcccttc tctcacagaa aatgaataaa
    10441 taaaactgct gcaccgcaag aatcgcgttc gatgtgacta tgatcgtatc cggtgagtac
    10501 agctttgatc caaaggctgg ttttctgtga tgattgacca gcctcagagt attgatgaca
    10561 ccaccgttca taacagatcg gcattactat gcataagtac tgtctcatat tctcgccaaa
    10621 acacttttta atgcctttgc gaattcatcc agacttatat tgtcaatttt ttgcccaacg
    10681 acgactgact ccgaaacatc ggccattgcg ggccatcgcg ttttgtaatc agcataaatt
    10741 gcaacaacgg gagtattata ggcacttgcc atatgtaata ttgaggtgtc aggagttatt
    10801 gcgatataag catcttttat gattgctgct gaacgtttaa tcgagggtga taaggataaa
    10861 cggtagacat tattataaca atccactaat accctggctt tatcttcccc ttttggtcca
    10921 tgaacgatca ctattggtat atctgtttca ctttgaattt ttgctattag attttctgca
    10981 atcgataatg aaaatgtacg ttcctgcgag ctaccttcta aattaagcgc aatgtaagat
    11041 cctaacgatc gcatttcctc gcgcacctca tcgagtacat cctcacttag tggcaattca
    11101 tatattggcc tgactgctgg aaaacccgcc tcacgcatca taaatgccca tgtcatgggt
    11161 acaggagccc ggagattctg atccatccgg gacgcgttct tgcacaaagg ggagtagcac
    11221 ttcatggtta aaccaacaac ctgaaaattc gtttttgctt tcaactgact gataaatatc
    11281 atcgttttca ggttcttttt acgcatcgcc tctatacaaa gatccggcgt accgtattgc
    11341 tgtgttatgt tctttgctaa atcttttatt tcttttaatg ttgcgtgatc ctgcatagtc
    11401 attgtgacta atgttaattt agtcttttca agtttaagcg cattaaagac ttctaaatta
    11461 attgtcgacg ttacaattaa aagatgctta attttatgca attcaagcgc ccgaataaca
    11521 ggaaagatgg ccatagcatc gccaatctga tcgggaatat ggatgacaac aaagtctgtt
    11581 ttttcaatat tgaaattata agctttataa tcgtagtaac taaatgcaat acgtctcaac
    11641 aatgatgcta aaaacatacc taacctcgcc tccctactgg ttataatgca aggcagtcta
    11701 tcagactcat cagggtgcca ttttgtgcat atgcggactt ttatgtttca tatctctaac
    11761 ctgtgggtcc tctgcttaat ccttaaacaa caccagcaac tcctgcgctt tcatcttcca
    11821 tcgaattttt catgttgccg ctaatcagcc ataaaatcat ttgcagatgc gctctgtcga
    11881 ggtagtctca taaggttcgt ttatagatcg acggcaatgt gagttacctt ttccatacta
    11941 attataaaaa gacagtacaa acaggatcat tatggactcc acgctcatct ccactcgtcc
    12001 cgatgaaggg acgctttcgt taagtcgcgc ccgacgagct gcgttaggca gcttcgctgg
    12061 tgccgtcgtc gactggtatg attttttact ctatggcatc accgccgcac tggtgtttaa
    12121 tcgcgagttt ttcccgcaag taagcccggc gatgggaacg ctcgccgcat ttgctacctt
    12181 tggcgtcgga ttccttttcc gtccgctcgg cggtgtcatt ttcggtcact ttggcgaccg
    12241 actgggacgt aagcgcatgt taatgctgac tgtctggatg atgggcatcg cgacagcctt
    12301 gattggtatt cttccttcat tctcgaccat tgggtggtgg gcacctattt tgctggtgac
    12361 actgcgtgcc attcagggat ttgccgtcgg cggcgaatgg ggaggcgcgg cgttgctttc
    12421 cgttgaaagt gcaccgaaaa ataaaaaagc cttttacagt agcggtgtac aagttggcta
    12481 cggtgtaggt ttactgcttt caaccggact ggtttcattg atcagtatga tgacgactga
    12541 cgaacagttt ttaagctggg gctggcgcat tcctttcctg tttagcatcg tactggtact
    12601 gggagcattg tgggtgcgca atggcatgga ggagtccgcg gaatttgaac aacagcaaca
    12661 ttatcaagct gccgcgaaaa aacgcatccc ggttatcgaa gcgctgttac gacatcccgg
    12721 tgctttcctg aagattattg cgctacgact gtgcgaattg ctgacgatgt acatcgttac
    12781 tgcctttgca cttaattatt caacccagaa tatggggcta ccgcgcgaac ttttccttaa
    12841 tattggtttg ctggtaggtg gattaagctg cctgacaatt ccctgttttg cctggcttgc
    12901 cgatcgtttt ggtcgccgta gggtttatat cacaggtgcg ttaatcggaa cgttgagcgc
    12961 atttcctttc tttatggcgc ttgaagcaca atctattttc tggatagttt tcttctccat
    13021 aatgctggca aacattgcgc atgacatggt ggtgtgtgtg caacaaccga tgtttaccga
    13081 aatgtttggt gccagttaac gctatagtgg cgctggagtc ggttatcagg ttgccagtgt
    13141 ggttggcggt ggatttacac cttttattgc cgctgcactc atcacttact ttgccgggaa
    13201 ctggcatagc gtcgccattt atttgctggc tggatgcctg atttccgcaa tgaccgcttt
    13261 gttgatgaaa gacagtcaac gcgcttgata gcctggcgaa gatcatccga tcttcgcctt
    13321 acacttttgt ttcacattcc tgtgacatac tatcggatgt gcggtaattg tatggaacag
    13381 gagacacaca tgaataataa gggctccggt ctgaccccag ctcaggcact ggataaactc
    13441 gacgcgctgt atgagcaatc tgtagtcgca ttacgcaacg ccattggcaa ctatattaca
    13501 agtggcgaat tacctgatga aaacgcccgc aaacaaggtc tttttgtcta tccatcactg
    13561 accgtaacct gggacggtag cacaaccaat ccccccaaaa cgcgcgcatt tggtcgtttt
    13621 actcacgcag gcagctacac caccacgatt actcgcccta ctctctttcg ttcgtatctc
    13681 aatgaacaac ttacgttgct gtatcaggat tatggtgcgc atatctcggt gcaaccctcg
    13741 cagcatgaaa tcccctatcc ttatgtcatc gatggctctg aattgacact tgatcgctca
    13801 atgagcgctg ggttaactcg ctacttcccg acaacagaac tggcgcaaat tggcgatgaa
    13861 actgcagacg gcatttatca tccaactgaa ttctccccgc tatcgcattt tgatgcgcgc
    13921 cgcgtcgatt tttccctcgc gcggttgcgc cattataccg gtacgccagt tgaacatttt
    13981 cagccgttcg tcttgtttac caactacaca cgttatgtgg atgaattcgt tcgttgggga
    14041 tgcagccaga tcctcgatcc tgatagtccc tacattgccc tttcttgtgc tggcgggaac
    14101 tggatcaccg ccgaaaccga agcgccagaa gaagccattt ccgaccttgc atggaaaaaa
    14161 caccagatgc cagcatggca tttaattacc gccgatggtc agggtattac tctggtgaat
    14221 attggcgtgg gaccgtcaaa tgctaaaacc atctgcgatc atctggcagt gctacgcccg
    14281 gatgtctggt tgatgattgg tcactgtggc ggattacgtg aaagccaggc cattggcgat
    14341 tatgtacttg cacacgctta tttacgcgat gaccacgttc ttgatgcggt tctgccgccc
    14401 gatattccta ttccgagcat tgctgaagtg caacgtgcgc tttatgacgc caccaagctg
    14461 gtgagtggca ggcccggtga ggaagtcaaa cagcggctac gtactggtac tgtggtaacc
    14521 acagatgaca ggaactggga attacgttac tcagcttctg cacttcgttt taacttaagc
    14581 cgggccgtag caattgatat ggaaagtgca accattgccg cgcaaggata tcgtttccgc
    14641 gtgccatacg ggacactact gtgtgtttca gataaaccgt tgcatggcga gattaaactt
    14701 cccggtcagg ctaaccgttt ttatgaaggc gctatttccg aacacctaca aattggcatt
    14761 cgggcgatcg atttgctgcg cgcagaaggc gaccgactgc attcacgtaa attacgaacc
    14821 tttaatgagc cgccgttccg ataataagtt ctgaaccttc tcatcagaaa tcgatgtgaa
    14881 agattaagta agacatctgg caaatagcct gcaatcaacg cgggctgttt gcttccttgg
    14941 gcagatgcga gtttcat
//
LOCUS       DAZKHC020000100        14672 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZKHC020000100 DAZKHC020000000
VERSION     DAZKHC020000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.100"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..648
                     /gene="ydcL"
                     /locus_tag="ACHOSM_RS18670"
                     /old_locus_tag="pgaptmp_003734"
     CDS             <1..648
                     /gene="ydcL"
                     /locus_tag="ACHOSM_RS18670"
                     /old_locus_tag="pgaptmp_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HGU4560154.1"
                     /translation="KVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKETTSATGKP
                     VLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIAQRK
                     PLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRLYFE
                     GELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK"
     gene            complement(747..1172)
                     /gene="tnpA"
                     /locus_tag="ACHOSM_RS18675"
                     /old_locus_tag="pgaptmp_003735"
                     /pseudo
     CDS             complement(747..1172)
                     /gene="tnpA"
                     /locus_tag="ACHOSM_RS18675"
                     /old_locus_tag="pgaptmp_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1180..2388
                     /gene="insQ"
                     /locus_tag="ACHOSM_RS18680"
                     /old_locus_tag="pgaptmp_003736"
     CDS             1180..2388
                     /gene="insQ"
                     /locus_tag="ACHOSM_RS18680"
                     /old_locus_tag="pgaptmp_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU4560155.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVRTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(2428..3648)
                     /gene="ydcO"
                     /locus_tag="ACHOSM_RS18685"
                     /old_locus_tag="pgaptmp_003737"
     CDS             complement(2428..3648)
                     /gene="ydcO"
                     /locus_tag="ACHOSM_RS18685"
                     /old_locus_tag="pgaptmp_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HGU4560156.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAATVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGV
                     CYVVLNLIADRNRY"
     gene            3701..4237
                     /gene="sutR"
                     /locus_tag="ACHOSM_RS18690"
                     /old_locus_tag="pgaptmp_003738"
     CDS             3701..4237
                     /gene="sutR"
                     /locus_tag="ACHOSM_RS18690"
                     /old_locus_tag="pgaptmp_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HGU4560157.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            4310..6271
                     /gene="rlhA"
                     /locus_tag="ACHOSM_RS18695"
                     /old_locus_tag="pgaptmp_003739"
     CDS             4310..6271
                     /gene="rlhA"
                     /locus_tag="ACHOSM_RS18695"
                     /old_locus_tag="pgaptmp_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HGU4560158.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKISSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(6363..6593)
                     /gene="yncJ"
                     /locus_tag="ACHOSM_RS18700"
                     /old_locus_tag="pgaptmp_003740"
     CDS             complement(6363..6593)
                     /gene="yncJ"
                     /locus_tag="ACHOSM_RS18700"
                     /old_locus_tag="pgaptmp_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HGU4560159.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            6815..6991
                     /gene="hicA"
                     /locus_tag="ACHOSM_RS18705"
                     /old_locus_tag="pgaptmp_003741"
     CDS             6815..6991
                     /gene="hicA"
                     /locus_tag="ACHOSM_RS18705"
                     /old_locus_tag="pgaptmp_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HGU4560160.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            7037..7453
                     /gene="hicB"
                     /locus_tag="ACHOSM_RS18710"
                     /old_locus_tag="pgaptmp_003742"
     CDS             7037..7453
                     /gene="hicB"
                     /locus_tag="ACHOSM_RS18710"
                     /old_locus_tag="pgaptmp_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HGU4560161.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            7532..8938
                     /gene="ydcR"
                     /locus_tag="ACHOSM_RS18715"
                     /old_locus_tag="pgaptmp_003743"
     CDS             7532..8938
                     /gene="ydcR"
                     /locus_tag="ACHOSM_RS18715"
                     /old_locus_tag="pgaptmp_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU4560162.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            9183..10328
                     /gene="ydcS"
                     /locus_tag="ACHOSM_RS18720"
                     /old_locus_tag="pgaptmp_003744"
     CDS             9183..10328
                     /gene="ydcS"
                     /locus_tag="ACHOSM_RS18720"
                     /old_locus_tag="pgaptmp_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HGU4560163.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            10346..11359
                     /gene="ydcT"
                     /locus_tag="ACHOSM_RS18725"
                     /old_locus_tag="pgaptmp_003745"
     CDS             10346..11359
                     /gene="ydcT"
                     /locus_tag="ACHOSM_RS18725"
                     /old_locus_tag="pgaptmp_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4560164.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            11360..12301
                     /gene="ydcU"
                     /locus_tag="ACHOSM_RS18730"
                     /old_locus_tag="pgaptmp_003746"
     CDS             11360..12301
                     /gene="ydcU"
                     /locus_tag="ACHOSM_RS18730"
                     /old_locus_tag="pgaptmp_003746"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4560165.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIILIALYLAFVKRLGAFDAL"
     gene            12291..13085
                     /gene="ydcV"
                     /locus_tag="ACHOSM_RS18735"
                     /old_locus_tag="pgaptmp_003747"
     CDS             12291..13085
                     /gene="ydcV"
                     /locus_tag="ACHOSM_RS18735"
                     /old_locus_tag="pgaptmp_003747"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4560166.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMELGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            13107..14531
                     /gene="patD"
                     /locus_tag="ACHOSM_RS18740"
                     /old_locus_tag="pgaptmp_003748"
     CDS             13107..14531
                     /gene="patD"
                     /locus_tag="ACHOSM_RS18740"
                     /old_locus_tag="pgaptmp_003748"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HGU4560167.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGGVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         3630 a         3573 c         3889 g         3580 t
ORIGIN      
        1 aaagttgcag ctttatgcgg cttgttggct ctgtctggtt gtgcctctaa aatcacccag
       61 ccagataaat attctggttt tttaaacaat tactctgatt taaaagaaac aacctcagca
      121 acaggtaaac ctgttttacg ttgggtagac ccgagttttg atcaaagcaa atatgacagc
      181 atcgtctgga acccaatcac ttattatccg gtgccaaaac cttctaccca ggtagggcag
      241 aaagttcttg ataaaatttt gaactatacc aacaccgaaa tgaaagaagc gatagcgcag
      301 cgtaaaccac tggttaccac cgctgggccg cgtagtctga ttttccgtgg ggccattacc
      361 ggtgtagata ccagcaaaga agggctgcaa ttctatgaag tggttcctgt tgcattagtg
      421 gttgcgggga cgcaaatggc tacaggccac cgtaccatgg atactcgcct ctattttgaa
      481 ggtgagctga ttgatgcagc gactaataaa ccggttatca aagtcgttcg tcagggcgaa
      541 ggtaaagacc tgaataacga aagtacgcca atggctttcg aaaatattaa acaagttatt
      601 gatgacatgg cgaccgatgc caccatgttt gacgttaaca aaaagtagtt ccagacgcgc
      661 catcgttaga tggcgctttt tatccggtgc gccgtaaaac cccatccttc agggcgggga
      721 tataaggcgc ggttttccac ctgaccaggt gtttgctgtt gttcaatgta ttggcggatg
      781 acggatattg gcgcacctcc gcagctactg gcaaaatagc caggactcca caaaacgcct
      841 ttgtagtaat acctgactgc aatatctggt cgatctcgtc gcagtcacct acccgatacg
      901 cctttgaggc tgtttaccag actggatatc gccagtttgg gaggatagtt aattaacaaa
      961 tggacgtgat ctggttcgcc atccatttca accagttcag cttcaaaatc agcacataca
     1021 tttgaaaagt aagtgcgtag tttttctgtc gcgtcatggt caaaaatctg acgtcggtat
     1081 ctggtgacaa agaccaggtg aacatgcatc aggaaaacac aatgccttcc acgccggata
     1141 tcggtttctt ttttcacaga ccaaagtatc atttggcctg tgaaacgact acaagcattt
     1201 aaattccagt taagacccgg tggtcaacag gagcgtgaaa tgaggcgctt cgcaggcgca
     1261 tgtcgtttcg ttttcaatcg tgctctggca cgtcagaatg agaatcatga ggccggtaat
     1321 aaatacatcc cttacgggaa aatggcttcc tggctggttg agtggaaaaa tgccactgaa
     1381 acgcaatggc ttaaagatgc cccgtcacag ccattgcaac agtcactgaa agaccttgag
     1441 cgggcttaca aaaacttctt ccagaatcgg gctgcttttc cccgattcaa aaagcgggga
     1501 cagaatgatg tattccgcta cccgcagggc gttaagctcg atcaggaaaa cagccgtatt
     1561 tttctgccga aactgggctg gatgcgctac cggaacagcc gtcaggtcac gggtgttgtg
     1621 aaaaatgtca ctgtcagcca gtcctgcggt aagtggtaca tcagtattca gacagaaagt
     1681 gaagtatcaa ctccggttca cccttcagca tcaatggtcg ggctggatgc tggcgtggct
     1741 aaactcgcca cgctgtcaga tggcacagtc tttgagcctg taaacagttt tcagaaaaac
     1801 cagaagaagc tggcgagact tcagcgacag ttaagccgca aggtcaaatt cagcaacaac
     1861 tggcagaagc agaaacgcaa aatacagcga ctgcattcct gtatcgcaaa tatccgcagg
     1921 gactaccttc acaaagtcag aacgaccgtc agcaaaaacc acgcaatgat agtcattgag
     1981 gatttgaagg tcagcaacat gtcaaagtca gcagcgggta cggtcagcca gccggggcgc
     2041 aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg gtatgaaatg
     2101 cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc tgttccgcca
     2161 gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa ccgcctgtca
     2221 caaagtaaat tcagatgcca ggtatgtgga tatacagcga acgccgatgt aaatggcgct
     2281 cgtaacattt tagctgcggg gcacgccgtt cttgcctgtg gagagatggt gcagtcaggc
     2341 cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac gtagcaggga
     2401 tcctcgtcct tcagggcgtg gaggatgtca atatcggttt ctgtcagcga ttaaattcaa
     2461 caccacgtaa caaacgcctc cggcaattaa tccccagaac gcagaaccaa tcccgaccag
     2521 cgtcaatcca cttgccgtta ccagaaatgc caccaccgcc gcgtcgcgct cacgctcacg
     2581 ctcattatgc agcgcctgat acaaactgcc gccaatggta ctcaacagcg ccagacctgc
     2641 cagcatctgg atccaactta cgggcagggc agccatcatc ccggtaatgg cactaccaaa
     2701 cagacctgcg agcaaataga aaatgcctgc aacggtagct gccagccaac gttgatcttt
     2761 atccggatgc gcttccgggc tttggcaaat agccgcggtg attgccgcaa taccgacgga
     2821 ataaacgccg aaaggggaaa aaaccagtgc cagcaatcca gtaaatacaa ttaatggcga
     2881 aacaggagcc gaatatccag ctgctttcat tgctgcgata cccggtgcgt tttgcgatgc
     2941 catcgtcacc agaaaaaggg ggagtgcaac gctcaggctg tgagcaaacg aaaaatcagg
     3001 ggtaatataa gtggggagaa cgggtttaaa gacaacatca gttgtgacaa cgtcaccttg
     3061 cgcgatgacg atcacgatcc caataatcat cgcggcaatt accgcatagc gcggcgcaac
     3121 ggccttggtt gccagccata ccagcaacat acttccacac aacgtaaatt gaccgtccag
     3181 actggcaaac gcctgtaaac caaagcgtaa taaaatcccg gcaagcattg ccgccgcaag
     3241 cgagtgcgga ataatgcgca tcagacgagc aaagagtccc gttatgccgc agaggactat
     3301 taacgcgttg gtgacaataa aaacgccgat ggcttcgtta agtgttagtc cctgcaatcc
     3361 ggtgaccaac aaagccgcgc caggcgttga ccatgcggtg agaacaggta cgcgatacca
     3421 taatgtcaga gtcagcgtac tgacgcccat tgccagcccc agcgccgtca tccagccaga
     3481 gatttgtgca gtggtggctc cggcgacaat cgctgcttgc cagattattg ccgctgaact
     3541 ggcgtagcca attaataccg ccagaaaccc cgccagtagc gtgggtggag gaatagaaaa
     3601 cagacgcata gtcacctcgt gcgttatagc gtacaggcac cgtagcattt gtccgttata
     3661 acgcacaagt gataaacttc cgttttgccg gaggagtcgc atggaaaatc tcgctcgctt
     3721 tttatccacc acacttaaac aactacgcca gcagcgcggc tggagtcttt cgcgactggc
     3781 agaagcgacg ggcgtttcta aagcaatgtt agggcagatt gagcgtaatg agtccagtcc
     3841 gacggtcgcg acattatgga aaattgccac cgggttgaac gtaccatttt caacatttat
     3901 ttctccgccg cagtccgcta cgccttcagt ttatgatcca caacagcagg cgatggtgat
     3961 cacttcgctg tttccttatg atccacaact ctgctttgaa cacttctcga ttcagatggc
     4021 atctggtgca ataagcgaat cgacgcccca tgagaagggg gtaatcgaac atgtcgtcgt
     4081 cattgatgga caacttgatc tgtgcgttga tggggaatgg cagaccctta attgtgggga
     4141 aggcgttcga tttgctgcag acgtcacgca tatctatcgt aatggtgggg agcaaaccgt
     4201 acattttcat tccctcatcc attacccgcg cagttaagcg ggaaaactat ttcgcaacgt
     4261 cgcgcttgtg gctaaaatag ccgccatttt tcagctactg gataagaatg tgaccgtatc
     4321 ttctcatcga cttgaactgt taagcccggc acgcgatgcc gccattgccc gcgaagctat
     4381 tttgcacggt gccgatgctg tttatatcgg cggccctggt tttggtgccc gtcataatgc
     4441 cagtaatagc ctgaaagata ttgccgagct ggtgccgttt gcccatcgtt atggtgcaaa
     4501 aattttcgtc acgcttaaca ccattttgca tgatgatgag ctggaacccg cgcaacggct
     4561 gattactgac ctctaccaga ccggtgtcga tgcgctgatt gttcaggata tggggattct
     4621 ggaacttgat attccgccga ttgaactgca cgccagtacg cagtgcgaca ttcgtacagt
     4681 tgaaaaagcg aagttcctct ctgatgttgg cttcacgcag attgtgctgg cgcgagagct
     4741 gaatcttgat cagatccgcg cgattcacca ggctacggac gcgaccattg aattctttat
     4801 tcatggggca ctgtgcgtgg cctattcggg tcagtgctac atttctcatg cgcaaacagg
     4861 gcgtagcgcc aaccgtggcg attgctcgca ggcgtgccgt ttgccataca cattgaaaga
     4921 cgatcagggg cgggtggttt cctatgaaaa acatctgctg tcgatgaaag ataacgatca
     4981 gactgccaac ctcggcgcgc tgattgatgc tggtgtacgc tccttcaaga ttgaagggcg
     5041 ttacaaagat atgagctacg tgaagaatat caccgcccat tatcgccaga tgctggatgc
     5101 cattattgaa gaacgtggcg atctgacgcg tgcttcatca ggtcgtactg aacatttctt
     5161 tgttccatcg acggaaaaga ctttccaccg tggtagcaca gattattttg tgaatgcccg
     5221 taaaggcgat attggcgcgt tcgattcgcc gaaatttatc ggcctgccgg tgggtgaagt
     5281 attgaaagtg gcgaaagatc atctcgatgt tgccgtcacc gagccactgg caaatggtga
     5341 tggcctgaac gtgatgatta aacgtgaagt catcggtttt cgtgccaata cggtcgagaa
     5401 aaccggagaa aatcagtacc gcgtctggcc caatgaaatg ccagcagatt tgcacaaaat
     5461 tcgtccacat cacccactaa accgtaatct tgatcataac tggcagcagg cactgacaaa
     5521 aatctccagc gaacgtcggg tggcggtaga cattgaactg ggcggctggc aggaacaact
     5581 gattctgacc ctcaccagtg aagagggtgt cagcatcacg catacgctgg acgggcagtt
     5641 cgacgaagcc aataacgccg aaaaagcaat gaacaatctg aaggatggtc tggcaaaact
     5701 ggggcaaacc ctctattacg cccgcgatgt gcaaattaat ttgccggggg cgctgtttgt
     5761 accaaacagt ctgttaaacc agttccgccg tgaagctgct gacatgctgg atgctgcgcg
     5821 tcttgccagt taccagcgcg gcagccgtaa accggttgct gatcctgcgc cggtttatcc
     5881 gcaaacgcat ctgagtttcc tcgcgaacgt atacaaccag aaagcgcgtg aattttatca
     5941 tcgctatggt gtgcagctga ttgacgcggc gtatgaggca catgaagaga agggcgaagt
     6001 cccggtgatg atcaccaagc attgtctgcg ctttgccttt aatctgtgcc cgaaacaggc
     6061 gaaaggcaat atcaaaagct ggaaggcgac gccaatgcaa ctggttaacg gcgatgaagt
     6121 attaacgcta aagtttgatt gccgcccatg cgagatgcac gtcattggca aaatcaaaaa
     6181 tcacatactg aaaatgccgt taccgggaag cgtagtggca tccgtaagtc cggatgagct
     6241 gctgaaaaca ttgcctaagc gaaaagggta aaacgccagt tttctggtta ctcacaactc
     6301 attgaatctg catgatattg cctgccgggt aaggcgtcac gccgcatccg gcaacaaatg
     6361 actcaacgtt gtcccgcttc tggtttgcgc gatttttgcc agtaaaaatg ctcgcgcaaa
     6421 ccttccgccg actcttccgc cacagcacgc aattcatcgc tgtccgcttc atgacgcagc
     6481 tgatgatcca cattctttac ccacacaaat tcatgtcctt tgtgccctgc catgagttgt
     6541 cctgagaaca gcgcacacgt taataagaca accgataacg ccttcgtaaa catcctgcca
     6601 cctttttctt accttttgcc gctatgatgc cgatcgtttc ttgaggttat tattcagttt
     6661 tgcaaattag cgcaaagaaa ttctggaatc ttccttcctg attttgcatg gcattctgcc
     6721 gttgcggcga tttagtgcta ttttcgagca aattacacac ggaggtaaac actaatgttt
     6781 actttgttga tatactcagc ggcagggagg cgatgtgaaa caaagcgagt tcagacgttg
     6841 gctcgaatct cagggcgtcg atgtagcgaa tggcagcaac catttgaaac tcaggtttca
     6901 tgggaggcgc agtgtcatgc cgcgtcaccc ctgcgatgag attaaagaac cattgcgtaa
     6961 agcaatcctg aaacaactcg gtttgagtta atcgccaatt aaaaaggtta atgacatgcg
     7021 agggacagtc gaaattatgc gttatcccgt cactcttaca cccgcgccgg aaggcggtta
     7081 tatggtttct tttgtggata tccctgaagc gttgacgcag ggcgaaactg tcgctgaagc
     7141 gatggaagcg gcaaaagatg ctttactgac cgcatttgat ttttattttg aagataacga
     7201 gcttatccct ttaccttcgc cattaaatag tcatgatcac tttattgaag tacctttgag
     7261 cgtcgcctct aaggtattgc tgttaaatgc ttttttacag tcagaaatca ctcagcaaga
     7321 gttagccagg cgaattggca aacctaaaca ggagattact cgcctattta acttgcatca
     7381 tgcgacaaaa atcgacgccg tccagctcgc ggcaaaggcg cttggcaaag agttatcgct
     7441 ggtgatggtt taattacagt taacgaaaag ttgtcatttt taacaactga tatagactgc
     7501 cgaatcatct gcacataatt acgattcgat aatgaaaaaa taccagcagc ttgcagaaca
     7561 attacgcgag cagattgcgt cgggtatctg gcaacccggc gatcgtttgc cttcgttgcg
     7621 tgaccaggtg gcgctttcag gcatgagctt tatgactgtc agccatgcct atcagttgct
     7681 cgaaagtcag ggatatatta tcgcacgacc gcagtcgggt tattacgttg cgccacaggc
     7741 aataaaaatg ccgaaagcgc cagtcattcc agtcactcga gatgaagcag tcgatatcaa
     7801 cacttatatt tttgatatgt tgcaggccag tcgcgatccg tcggtcgttc cgtttgcctc
     7861 ggcctttccc gacccgcgac ttttccccct ccaacaacta aaccgctcgc tggcgcaggt
     7921 aagcaaaacc gccacagcga tgagcgtgat tgaaaactta ccgccaggaa acgcagaact
     7981 gcgtcaggct attgctcgtc gctatgcctt acagggcatc accatttctc ctgatgaaat
     8041 tgtcattact gccggggcgt tagaggcatt aaacctcagt ttgcaagcgg taactgaacc
     8101 gggcgattgg gtgatagtag agaatccttg tttctacggt gcgttgcagg cgctggagcg
     8161 gctacggctg aaggcgttat cggtggcgac ggatgttaaa gaagggatcg atcttcaggc
     8221 gctggaactg gcgttgcagg attatccggt gaaagcgtgc tggctgatga ctaatagcca
     8281 gaacccactc ggatttacct taacgccgca aaaaaaagca caactggtgg cgttactcaa
     8341 tcagtacaac gtaacgctga ttgaagatga cgtttacagc gaactttatt ttggacggga
     8401 aaaaccgctg cctgcgaaag cgtgggatcg ccacgatggc gttttgcatt gctcttcgtt
     8461 ttcgaaatgt ctggtgcctg gttttcgtat tggttgggtc gccgccggaa aacatgcacg
     8521 taaaattcaa cgcttgcagt tgatgagtac gctttccacc agctcaccga tgcaacttgc
     8581 gctggtggat tacctttcca cgcgccgata cgacgcccat cttcgtcgcc tgcgtcgcca
     8641 gcttgcggaa cgtaaacaac gtgcctggca ggcactgctg cgttatctgc ctgcggaagt
     8701 gaaaattcat catagtgaca gcggttattt tctctggctg gagctccccg agccgttaga
     8761 tgccggcgaa ttaagcctgg cggcactgac gcatcatatc agtattgcgc cgggtaaaat
     8821 gttttctacc ggtgaaaact ggtcacgttt ttttcgtttt aataccgcgt ggcagtgggg
     8881 agaacgtgaa gaacaggcgg taaaacaatt aggcaaactt attcaagaac ggctgtaata
     8941 gcgtttaatt taattcctct tagattgggt aatatgaatt tcgaatagca gtcatatttc
     9001 ctaacccctt gtctatactc cagaagataa ccttacagac ggcataatgc gcggtagctc
     9061 acaacctgat taaattttct caggggcgaa ggtgtgcctg caagccgccg tctatggtta
     9121 aacaaggaga tatttttacg gcacggcggc tgaacaatta attacgacag gagtaagacc
     9181 ttatgagcaa gacatttgcc cgcagcagcc tgtgtgcgct caccatgaca ataatgaccg
     9241 ctcacgccgc cgaaccacct accaatttag ataaaccgga agggcgactg gatattatcg
     9301 cctggccggg atacatcgaa cgcggacaaa ctgataaaca atacgactgg gtaacgcaat
     9361 ttgaaaaaga gacaggctgc gcggtgaatg tgaaaaccgc cgcgacttcc gatgaaatgg
     9421 tcagtctgat gaccaaaggg ggttacgatc tggttacggc atccggcgat gcctcgctgc
     9481 gtttgattat gggcaaacgc gtgcagccga ttaataccgc cttgattccc aactggaaaa
     9541 cgctcgatcc gcgcgtggtt aaaggcgact ggtttaatgt tggcggcaaa gtttacggca
     9601 caccttacca atgggggccg aacctgctga tgtacaacac taaaaccttc ccgacgccgc
     9661 cggatagctg gcaagtggtt tttgttgagc aaaatctgcc ggacggcaag agcaataaag
     9721 gccgcgttca ggcttatgat ggccctatct acattgcgga cgctgcgttg ttcgttaaag
     9781 ccactcagcc gcagttgggc atcagcgatc cgtatcaact aaccgaagaa cagtaccagg
     9841 cggtgctgaa agtgctgcgc gctcaacaca gtttgatcca tcgctactgg catgacacta
     9901 ccgtgcaaat gagcgatttc aaaaacgagg gtgtggttgc ttccagtgcc tggccctatc
     9961 aggccaacgc cctgaaagcc gaaggccagc ctgttgctac cgttttcccg aaggagggtg
    10021 ttaccggttg ggctgatacc accatgctgc atagcgaagc gaaacatccg gtttgcgcct
    10081 acaaatggat gaactggtca ttaacgccaa aagtgcaggg cgatgtggcg gcctggtttg
    10141 gctcgttacc ggtagtgccg gaagggtgta aagccagtcc gttattaggc gaaaaaggtt
    10201 gtgaaaccaa cggttttaac tatttcgaca aaatcgcctt ctggaaaacg cctatagcag
    10261 aagggggcaa gtttgttccc tacagtcgct ggacgcagga ttacattgcc attatgggcg
    10321 gtcgctaact tcgctggggt gctttatgac gtacgcagtg gagtttgaca acgtctcgcg
    10381 gttgtacggt gacgtgcgcg cagtagatgg cgtcagtatt gcgataaaag atggtgagtt
    10441 cttctctatg ctggggccgt ccggctccgg caaaaccacc tgcctgcgcc tgattgctgg
    10501 cttcgaacag ctttccggcg gggctatctc tatctttggt aaacccgcca gcaatctgcc
    10561 accgtgggag cgggacgtga atactgtctt tcaggactac gcgctatttc cgcatatgtc
    10621 gattcttgac aatgtcgcct atgggctgat ggtcaaaggc gtgaataaaa agcagcggca
    10681 cgcaatggcg caagaggcgc tggagaaagt ggcgttgggg tttgtacagc aacgtaaacc
    10741 gtcacaactt tctggtggtc agcgccagcg ggttgctatc gccagagcat tggtgaatga
    10801 accgcgcgta ttgctgttgg atgaaccgct cggcgcactg gatctcaaat tgcgtgagca
    10861 gatgcagctg gaactgaaaa aactgcaaca gtctctcggt atcactttta tcttcgttac
    10921 ccacgatcag ggcgaagcgt tatcgatgtc cgatcgtgtg gcggttttca ataatggacg
    10981 cattgagcag gtcgattccc cgcgcgatct ctatatgcgc ccgcgcacgc cgtttgttgc
    11041 cgggttcgtt ggtacatcga atgtttttga tggactgatg gcagagaaac tttgtggcat
    11101 gacgggaagc ttcgccctgc gaccggaaca tatccgcctc aacacccctg gtgaactgca
    11161 ggccaatggc acgatccagg cggtgcaata tcagggcgcg gcaactcgtt ttgaactgaa
    11221 attgaacggc ggtgaaaaac tacttgtgag tcaggccaat atgacaggcg aagaactgcc
    11281 tgccacgctc acgcccggac aacaggtgat ggtttcctgg tcgcgtgatg tgatggtgcc
    11341 gctggttgag gagaggtgaa tggcgatgaa tgtattgcaa tcaccttcac gtccaggtct
    11401 gggtaaggtg tccggtttct tctggcgtaa tccggggctg gggctgtttt tactgctgct
    11461 tggcccgcta atgtggtttg gcattgtcta tttcggctcg ctgctgacac tgttatggca
    11521 gggattttat acttttgacg atttcaccat gtcggtaacg ccggaactga cgctggcgaa
    11581 tatccgtgcg ctgtttaatc cggcgaatta cgacatcatt ctccgcacgc tgactatggc
    11641 tgtggcagtc actatcgcca gcgccattct ggcttttcca atggcgtggt atatggcgcg
    11701 ctataccagc gggaaaatga aagcgttttt ttatattgcg gtaatgttgc cgatgtgggc
    11761 gagctacatt gttaaagcct atgcctggac gttattgctg gcaaaagatg gcgtggctca
    11821 gtggttttta caacatcttg ggctggaacc actgctgact gcgttcctta cattacctgc
    11881 ggtgggcgga aatacgctgt caacttccgg gctggggcgc tttctggtgt ttctctatat
    11941 ctggttgccg ttcatgatcc tgcccgttca ggcggcgctt gagcgtttgc cgccgtcatt
    12001 gttgcaggcg tcggctgatc tcggcgcacg tccacgacaa acctttcgct atgtggtgct
    12061 gccgctggca atcccgggta ttgccgctgg ctctatcttt accttctcac tcacactggg
    12121 cgattttatc gtcccgcagc tggttggtcc tccaggatat tttatcggca atatggttta
    12181 ttcccagcag ggggcgattg gcaatatgcc gatggcggcg gcattcaccc tggtgccgat
    12241 tattctcatc gcactgtacc tggcgttcgt gaaacgtctg ggagcgttcg atgcactctg
    12301 aacgcgcacc gtttttcctc aaactggcgg cctggggcgg cgttgttttc ctacattttc
    12361 ccatcctgat aatcgccgcc tatgcgttta acactgaaga tgcggcgttt agttttccac
    12421 cgcagggcct gacgctgcgc tggtttagcg tggcagcaca gcgtagtgat attcttgatg
    12481 ccgtgacact gtcacttaaa gtggcggcgc tggcgacatt aattgcgctg gtgttaggga
    12541 cgctggcagc tgccgcgctg tggcgacgag actttttcgg caaaaacgcc atttcgctgt
    12601 tactgctgct gcccattgcg ctgccgggca ttgtcactgg tctggcgtta ttaaccgcct
    12661 ttaaaaccat caatctggag ccgggatttt tcaccatcgt ggtcggtcat gcgacttttt
    12721 gtgtagttgt ggtgtttaac aatgtcatcg cccgttttcg ccgcacctcc tggagtctgg
    12781 ttgaggcgtc aatggagctt ggggccaatg gctggcaaac cttccgctac gtagtgttgc
    12841 cgaatctcag ttcggcgtta ctggcaggag gaatgctggc gtttgccttg tcgttcgatg
    12901 aaatcatcgt tacgaccttt acggcaggtc atgaacgaac gttaccgttg tggttgctca
    12961 atcagcttgg gcgaccgcgt gatgtaccgg taactaacgt ggtggcactg ctggttatgt
    13021 tggtaacaac cttgccgatc ctgggggcct ggtggctaac ccgcgaaggc gacaatggtc
    13081 aataaccact gatacaggaa tatgctatgc aacataagtt actgattaac ggagaactgg
    13141 ttagcggcga aggggaaaaa cagcctgtct ataatccggc aacgggggac gttttactgg
    13201 aaattgccga ggcatccgca gagcaggtcg atgctgctgt gcgcgcggca gatgcagcat
    13261 ttgccgaatg ggggcaaacc acgccgaaag tgcgtgcgga atgtctgctg aaactggctg
    13321 atgttatcga agaaaatggt caggtttttg ccgaactgga gtcccgtaat tgtggcaaac
    13381 cgctgcatag tgcgttcaat gatgaaatcc cggcgattgt cgatgttttt cgctttttcg
    13441 cgggtgcggc gcgctgtctg aatggtctgg cggcaggtga atatcttgaa ggtcatactt
    13501 cgatgatccg tcgcgatccg ttggggggcg tggcttctat cgcaccgtgg aattatccgc
    13561 tgatgatggc cgcgtggaaa cttgctccgg cgctggcggc agggaactgc gtagtgctta
    13621 aaccatcaga aattaccccg ctgaccgcgt tgaagttggc agagctggcg aaagatatct
    13681 tcccggcagg cgtgattaac atactgtttg gcagaggcaa aacggtgggt gatccgctga
    13741 ccggtcatcc caaagtgcgg atggtgtcgc tgacgggctc tatcgccacc ggcgagcaca
    13801 tcatcagcca taccgcgtcg tccattaagc gtactcatat ggaacttggt ggcaaagcgc
    13861 cagtgattgt ttttgatgat gcggatattg aagcagtggt cgaaggtgta cgtacatttg
    13921 gctattacaa tgctggacag gattgtactg cggcttgtcg gatctacgcg caaaaaggca
    13981 tttacgatac gctggtggaa aaactgggtg ctgcggtggc aacgttaaaa tctggtgcgc
    14041 cggatgacga gtctacggag cttggacctt taagctcgct ggcgcatctc gaacgcgtca
    14101 gcaaggcagt agaagaggcg aaagcgacag ggcacataaa agtgatcact ggcggtgaaa
    14161 agcgcaaggg taatggctat tactatgcgc cgacgctgct ggctggcgca ttacaggacg
    14221 atgccatcgt gcaaaaagag gtatttggtc cagtagtgag tgttacgctc ttcgacaacg
    14281 aagaacaggt ggtgaactgg gcgaatgaca gccagtacgg acttgcatct tcggtatgga
    14341 cgaaagatgt gggcagggcg catcgcgtca gcgcacggct gcaatatggt tgtacctggg
    14401 tcaataccca tttcatgctg gtaagtgaaa tgccgcacgg tgggcagaaa ctttctggtt
    14461 acggcaagga tatgtcactt tatgggctgg aggattacac cgtcgtccgc cacgtcatgg
    14521 ttaaacatta agttagatgt tagtggctgt tataggacgg ccactatcgt tttaggttct
    14581 gagtttaatg gcggttaagt attagaatga ttacattaat aactagcgta aaaattaggc
    14641 taaatgtttg atggcgattt ttcatcctgt ca
//
LOCUS       DAZKHC020000101        14511 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZKHC020000101 DAZKHC020000000
VERSION     DAZKHC020000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.101"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            385..1503
                     /locus_tag="ACHOSM_RS18745"
                     /old_locus_tag="pgaptmp_003749"
     CDS             385..1503
                     /locus_tag="ACHOSM_RS18745"
                     /old_locus_tag="pgaptmp_003749"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HGU4560168.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1569..1817
                     /locus_tag="ACHOSM_RS18750"
                     /old_locus_tag="pgaptmp_003750"
     CDS             1569..1817
                     /locus_tag="ACHOSM_RS18750"
                     /old_locus_tag="pgaptmp_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HGU4560169.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1882..2250
                     /locus_tag="ACHOSM_RS18755"
                     /old_locus_tag="pgaptmp_003751"
     CDS             1882..2250
                     /locus_tag="ACHOSM_RS18755"
                     /old_locus_tag="pgaptmp_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU4560170.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2344..2997
                     /gene="nfsB"
                     /locus_tag="ACHOSM_RS18760"
                     /old_locus_tag="pgaptmp_003752"
     CDS             2344..2997
                     /gene="nfsB"
                     /locus_tag="ACHOSM_RS18760"
                     /old_locus_tag="pgaptmp_003752"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HGU4560171.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3105..4352
                     /gene="ybdG"
                     /locus_tag="ACHOSM_RS18765"
                     /old_locus_tag="pgaptmp_003753"
     CDS             3105..4352
                     /gene="ybdG"
                     /locus_tag="ACHOSM_RS18765"
                     /old_locus_tag="pgaptmp_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HGU4560172.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4420..5796)
                     /gene="pheP"
                     /locus_tag="ACHOSM_RS18770"
                     /old_locus_tag="pgaptmp_003754"
     CDS             complement(4420..5796)
                     /gene="pheP"
                     /locus_tag="ACHOSM_RS18770"
                     /old_locus_tag="pgaptmp_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HGU4560173.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLLVWIVFLFV
                     AFKTLRRK"
     gene            complement(5898..9041)
                     /gene="cusA"
                     /locus_tag="ACHOSM_RS18775"
                     /old_locus_tag="pgaptmp_003755"
     CDS             complement(5898..9041)
                     /gene="cusA"
                     /locus_tag="ACHOSM_RS18775"
                     /old_locus_tag="pgaptmp_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HGU4560174.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKQPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPAYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPFNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9053..10276)
                     /gene="cusB"
                     /locus_tag="ACHOSM_RS18780"
                     /old_locus_tag="pgaptmp_003756"
     CDS             complement(9053..10276)
                     /gene="cusB"
                     /locus_tag="ACHOSM_RS18780"
                     /old_locus_tag="pgaptmp_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HGU4560175.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GVLERMRSESATHAH"
     gene            complement(10292..10624)
                     /gene="cusF"
                     /locus_tag="ACHOSM_RS18785"
                     /old_locus_tag="pgaptmp_003757"
     CDS             complement(10292..10624)
                     /gene="cusF"
                     /locus_tag="ACHOSM_RS18785"
                     /old_locus_tag="pgaptmp_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HGU4560176.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAKPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10648..12031)
                     /gene="cusC"
                     /locus_tag="ACHOSM_RS18790"
                     /old_locus_tag="pgaptmp_003758"
                     /pseudo
     CDS             complement(10648..12031)
                     /gene="cusC"
                     /locus_tag="ACHOSM_RS18790"
                     /old_locus_tag="pgaptmp_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
     gene            12188..12871
                     /gene="cusR"
                     /locus_tag="ACHOSM_RS18795"
                     /old_locus_tag="pgaptmp_003759"
     CDS             12188..12871
                     /gene="cusR"
                     /locus_tag="ACHOSM_RS18795"
                     /old_locus_tag="pgaptmp_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HGU4560177.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            12861..14308
                     /gene="cusS"
                     /locus_tag="ACHOSM_RS18800"
                     /old_locus_tag="pgaptmp_003760"
                     /pseudo
     CDS             12861..14308
                     /gene="cusS"
                     /locus_tag="ACHOSM_RS18800"
                     /old_locus_tag="pgaptmp_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
BASE COUNT         3483 a         3913 c         3576 g         3539 t
ORIGIN      
        1 ggttgataga aatatcgtcc tggggcttca tctttccaga cctcagagta aacctgaaac
       61 cggaaagctt caggcacccg cagttatctt acccttgctt agcaaaatag ctgtttattt
      121 atacagtaag tcctgtttcg gacttctcac tccccatttg atcaactgaa gctgaacccg
      181 tttttttctg catgcctcaa tcgtcaaaat tcttcgccac cagtcagcac tgccataact
      241 tgtcacattg caaaagtagc caaattgctt ctgtcataat cagttcacca tcaccaaaaa
      301 aaagaaacat tccagaactt ccgcatctcg cccagccgct cccacttcta tactgaatag
      361 aaaacgccaa cataagagaa acctatgcca ttacccgatt ttcatgtttc tgaacctttt
      421 acactcggta ttgaactgga aatgcaggtg gttaatccgc cgggctatga cttaagccag
      481 gactcttcaa tgctgattga cgcggttaaa aataagatca cggccggaga ggtaaagcac
      541 gatatcaccg aaagtatgct ggagctggcg acggatgttt gccgtgatat caaccaggct
      601 gccgggcaat tttcagcgat gcagaaagtc gtattgcagg cagccgcaga ccatcatctg
      661 gaaatttgcg gcggtggcac acacccgttt cagaaatggc agcgacagga ggtatgcgat
      721 aacgagcgct atcaacgaac gctggaaaac tttggttatc tcattcaaca ggcgaccgtt
      781 tttggtcagc atgttcatgt tggctgcgcc agtggcgatg acgccattta tttgctgcac
      841 ggcctgtcac ggtttgtgcc gcacttcatc gccctttccg ccgcgtcgcc atatatgcaa
      901 ggaacggata cgcgttttgc ctcctcacga ccgaatattt tttccgcctt tcccgataat
      961 ggcccgatgc cgtgggtcag taactggcaa caatttgaag ccctgtttcg ctgtttgagt
     1021 tacaccacga tgatcgacag cattaaagat ctgcactggg atattcgccc cagtcctcat
     1081 tttggcacgg tggaggttcg ggtgatggat accccgttaa cccttagcca cgcggtaaat
     1141 atggcgggat taattcaggc taccgcccac tggttactga cagaacgccc gttcaaacat
     1201 caggagaaag attacctgct gtataaattc aaccgtttcc aagcctgtcg ctatgggctg
     1261 gaaggcgtca ttaccgatcc gcacactggc gatcgtcgac cgctaacgga agacaccttg
     1321 cgattgctgg aaaaaatcgc cccttccgca cataaaattg gtgcatcgag cgcgattgag
     1381 gccctgcatc gccaggtcgt cagcggtctg aatgaagcgc agctgatgcg cgatttcgtc
     1441 gccgatggtg gctcgctgat tgggctggtg aaaaagcatt gtgagatctg ggccggtgac
     1501 taagtaggga attgccttac agaagaatat ccctgacaat ggtgttttaa cttacattta
     1561 acaatgttat gaaacaccct ttagaaacct tgactaccgc agcaggcatt ttgctgatgg
     1621 ctttcctctc ttgcctgctg ctgcccgccc ccgcactggg gctgacgctg gcacaaaaac
     1681 tggtgaccat gttccatctg atggatctta gccagcttta cactttattg ttttgtctgt
     1741 ggtttttagt gctgggcgct attgagtatt ttgttctgcg ctttatctgg cgacgctggt
     1801 tctcgctggc ggattaaacg tcgattatcg ccgtacttcg cggcatactt tgcttattct
     1861 ccttcgccta aaggaacgtt tatggataag caatcactgc acgaaacggc gaaacgcctg
     1921 gcccttgagt taccctttgt cgagctttgc tggccttttg gcccggagtt cgatgttttt
     1981 aaaattggcg gcaaaatttt tatgctgtcg tcggagctgc gcggcgtccc ctttatcaat
     2041 ctgaagtccg atccacaaaa atccctgtta aatcagcaga tatacccgag cattaagcca
     2101 gggtatcaca tgaataaaaa gcactggatt tcggtgtatc ccggcgagga aatctccgaa
     2161 gcgttacttc gcgatctgat caacgattcg tggaatctgg tggttgatgg tctggctaaa
     2221 cgcgatcaaa aaagagtgcg tccaggctaa agcggaaatc tatagcgcat ttttctcgct
     2281 taccatttct cgttgaacct tgtaatctgc tggcacgcaa aattactttc acatggagtc
     2341 tttatggata tcatttctgt cgccttaaag cgtcattcca ctaaggcatt tgatgccagc
     2401 aaaaaactta ccccggaaca ggccgagcag atcaaaactc tcctgcaata cagcccatcc
     2461 agcaccaact cccagccgtg gcattttatt gttgccagca cggaagaagg taaagcgcgt
     2521 gttgccaaat ccgctgccgg taattatgtg ttcaacgaac gtaaaatact tgatgcctcg
     2581 cacgtcgtgg tgttctgtgc aaaaaccgcg atggacgatg cctggctgaa gctggttgtt
     2641 gaccaggaag atgccgatgg ccgctttgcc acgccggaag cgaaagccgc gaacgataaa
     2701 ggtcgcaagt tcttcgctga tatgcaccgt aaagatctgc atgatgatgc agagtggatg
     2761 gcaaaacagg tttatctcaa cgtcggtaac ttcctgctcg gcgtggcggc tctgggtctg
     2821 gacgcggtac ccatcgaagg ttttgacgcc gccatcctcg atgcagaatt tggtctgaaa
     2881 gagaaaggct acaccagtct ggtggttgtt ccggtaggtc atcacagcgt tgaagatttt
     2941 aacgctacgc tgccgaaatc tcgtctgccg caaaacatca ccttaaccga agtgtaattc
     3001 tctcttgccg ggcatctgcc cggctatttc ccctcagatt ctcctgattt gcataaccct
     3061 gtttcagccg tcatcatagg ctgctgttgt ataaaggaga cgttatgcag gatttaatat
     3121 cccaggttga agatttagcg ggtattgaga tcgatcacac cacctcgatg gtgatgattt
     3181 tcggtattat ttttctgacc gccgtcgtgg tgcatattat tttgcattgg gtggtactgc
     3241 ggaccttcga aaaacgtgcc atcgccagtt cacggctttg gttgcaaatc attacccaga
     3301 ataaactctt ccaccgttta gcttttaccc tgcaggggat tatcgtcaat attcaggcgg
     3361 tattctggct gcaaaaaggc accgaagcgg cagatattct gactacctgc gcgcagttgt
     3421 ggatcatgat gtatgcgctg ctttcagtct tctcgttgct ggatgtgatt ttgaatctgg
     3481 cgcagaaatt cccggcggca tctcagttac cgctgaaagg gatatttcag gggattaaac
     3541 tgatcggcgc gattctggtc ggcattttga tgatctcgct gctgattggt cagtcgccag
     3601 cgattctgat cagcggtctt ggtgcaatgg ctgccgtgct gatgttggtt tttaaagatc
     3661 cgattcttgg tctggtggca ggtattcagc tttccgcgaa cgatatgctg aaactgggcg
     3721 actggctgga gatgccgaaa tacggcgcgg atggcgcggt gatcgatatt gggttaacca
     3781 ccgtcaaagt gcgtaactgg gacaatacca ttaccactat tcccacctgg tctctggttt
     3841 ctgactcctt taaaaactgg agcgggatgt cagcatctgg cgggcgacgt attaagcgca
     3901 gtatcagtat tgatgtcacc agtattcgtt ttcttgatga agacgaaatg caacgtctga
     3961 ataaagcgca cttgttaaaa ccttatttaa ccagccgcca tcaggaaatt aatgagtgga
     4021 atcgccagca aggttctacg gagtcgatat taaacctgcg ccgaatgacc aatattggaa
     4081 cctttcgtgc ctatctgaac gaatatctgc gtaaccatcc gcggattcgt aaagatatga
     4141 ctttaatggt acgccaactg gctccaggtg ataacggttt accgctcgag atctatgcgt
     4201 ttaccaacac cgtggtgtgg ctggaatatg aaagcattca ggctgatata ttcgatcaca
     4261 tatttgcgat tgtcgaagag tttggtctgc gacttcatca gtcgccaacc ggcaatgata
     4321 ttcgctctct ggcgggtgca tttaagcagt aattaaaaaa accgctctca tcgaatggat
     4381 gagagcggac atcgttttgt cggatgtagc gtgaatgcct tatttccgac gcagcgtttt
     4441 aaatgccaca aacaggaata caatccacac cagcagcagg atcgctgaca agcgcatatc
     4501 atccatcgtg cacatcagca gcaaaatcat gccgaggaag gcaatgcaaa gatagttgcc
     4561 gaacggatag agcagcgcct taaactgtgt ttcacgcccc tgacgtcgca tcgctgcacg
     4621 aaaacgcaga tgcgccaggc agatcataat ccagttcaac agcagcgttg ctaccaccag
     4681 cgccatcagc agaccaaacg ctttttgcgg cagcagatag ttgattaata ccaccagcga
     4741 agtgatcgct ccggaaagca tcagcgaatt aatcggcaca ccgcgacggc tgacgcgagt
     4801 caaaaacttc ggcgcattac cctgcacaga aaggccaaac agcatgcggc tgttagagta
     4861 aaccccgctg ttatacactg acagcgatgc taccagaatg acgaagttca gcgcagaagc
     4921 taccacgttg ctgtcgagat tatggaaaat catcacaaac gggctactgt tggatttcac
     4981 ttccacccac ggatagagcg ccagtaaaac caccagtgaa ccgatgtaaa acagcaggat
     5041 gcgatacacc acctgattta ccgcttttgg aatgcttttt tccggatcgc gcgcttcagc
     5101 ggcagtaatc ccaatcagct ccagaccgcc gaaggagaac ataattaccg ccagcgacaa
     5161 aatcagccca ttccagccgg tggcaaagaa accaccgtag cgccagaggt tgtcgatact
     5221 ggctttctcg ccgccgtgac cagaaaacag cagccacagg ccaaagccga tcataccgat
     5281 gatcgccagc actttaatca gcgcaaacca gaactcagtt tcaccatata agcgcacgtt
     5341 caccaggtta acggcgttga tgataataaa gaaggcggca gcccaaatcc acgttggaac
     5401 atccgggaac cagtactgca tatagatgcc cgcagcggtc agctctgcca tccccaccag
     5461 cacgaacatc acccagtagt tccagccaga gaggaagccc gcgaacggtc cccagtattt
     5521 ataggcaaag tgggcaaacg aaccggatac cggctcttcg accaccatct cgccgagctg
     5581 gcgcataatc aggaaagcga tgatcccggc gacgccgtag cccagcaata cagccggacc
     5641 cgccatctga atcgccgggc caatgccaag aaacagacca gtaccaattg cgccacccag
     5701 cgcaatcagt tgaatatgac ggttatgtaa tccgcgatga agcgtcggct cttgattcga
     5761 cgcagtatct tccgatacgg ttgacgcgtt tttcacgcct ttcccctgtg tgtctttttt
     5821 gttgaggggc accttttaac atttagtgcc catcgtcgca agacacaatc cacacggtta
     5881 aacggggtat cctgctttta tttccgtacc cgatgtcggt gcagccacat cagcttatac
     5941 gccgccggga taataaacag cgacagcaaa ggtgcggtga tcatgccgcc aatcatcggt
     6001 gcggcaatcc ggctcatcac ctctgaccct gctcctgttc cccacagaat cggcagcaga
     6061 ccagcgataa tcaccgccac cgtcatcgct ttcgggcgca cgcgcagtac cgcgccgtga
     6121 tacaacgcct catccagctt ctgctcgctg aatgtttgcg gattattcaa cgacggctcg
     6181 gcctctatag cgtgacgtaa atacatcagc atcaccacgc caaattcggc ggcgaccccg
     6241 gcaagggcaa taaagccagt acccgtcgcc acggaaagat gaaagcccat ccaccacagc
     6301 aaccagatgc cgcccaccag cgcaaacggt acgctgctga taatcagcaa cgcttcgccc
     6361 acccgacgga atgccagata caacagcacg aagatgatca tcagtgtcat cggcaccatg
     6421 agtttcagct tatggttggc gcgctccagt agctcgaact gcccggagaa tgccacgctg
     6481 gtgcccggtt ttaactgcac tttttcagct atcgcttttt gcaaatcgtg aaccaccgac
     6541 accatgtcac gatcgcgggc atcgatataa atccagctcg tcgggcgcgc attctcggtt
     6601 ttcagcatcg acggtccggt agagacttta atgtcggcca cgtctgccag ggtgatttgc
     6661 tgcttcatcg gcgtcaggat cggcaactgg cgcagtgcct gcggactatc gcgccagctt
     6721 tgcggataac gcagattaat tggataacgg gcaatccctt ccaccgtttc gccaaccatc
     6781 gccccgccca ccgcagatgt cacaaacaac tgcacatccg ctaccgtcat accgtaacgt
     6841 gcggcttttt cacggttaat ctcaacgttg atatagcgcc caccttccag ccgctcggca
     6901 agcgcagaag ctacgcctgg caccgttcgc gctacttctt caatttgctc agccatcgcg
     6961 tcgatatccg ccagcacagt gccggaaact ttaatgccga tggggctttt aatgccggtt
     7021 gagagcatat cgatacggtt acgaattggc ggcacccaca gattcgccag ccccggcagc
     7081 cgcacggtgt tatccagttc ctcaatgatt ttgtccatcg tcatgcctgg ccgccactgc
     7141 tcctgcggct taagctggat ggtcgtttct accatctcca gcggagcaga atcggtggcg
     7201 gtttccgctt tcccggtttt gccaaatacc cgcgccactt caggtacgct cataattagc
     7261 ttgtcggttt tttgcagcat actcgccgcc tctgctgcgg aaatcccagg cagcgtcgat
     7321 ggcatataca acaagtcgcc ttcattgatc tgcggtaaaa attccccgcc aactttattg
     7381 agcggccaga gaaccgtcag caccgaaagc gccgccacca gcagcgtggt tttcggccag
     7441 tgcagtactt tcagcaacag cggatgataa acacgaatca aaaagcgatt gaacgggtta
     7501 ctgctttccg gcggaatttt gccacggatc cagtacccca tcaggatagg gatcactacg
     7561 atcgccagca gcgccgcgcc cgccatcgca taggttttag tgaacgccaa cgggccaaac
     7621 agacgccctt cctgcccttc cagggtgaag atcgggataa acgacaacgt gataatcagc
     7681 agactgataa atagcgccgg acccacttca acagatgcat cggtgatcac ctgccagcgc
     7741 gttttattat ccagcgtggc gtcaggatgc tggtgctgcc actcttccag ccgtttatgt
     7801 gcattttcga tcatgacgat agcggcatcg accatcgccc cgacggcaat cgcaatgccg
     7861 cccaacgaca taatattggc attcagtccc tggaagtgca tgacaataaa agcaatacac
     7921 aaccccagcg gcaacgaaat aatcgccacc agcgccgagc gcacatgcca gagaaacagc
     7981 gcacagacta ccgccaccac aataaactct tccagcaact tgccgctgag gttgtcgatg
     8041 gcgcggtcaa tgagctggct gcgatcgtat gttgtaacta tctccacgcc ttccggcaga
     8101 ctacttttca gcgtttccag tttgtccttc acggcggcga tcacttctcg ggcgtttttg
     8161 ccggatcgca ggatcaccac cccgcccgcc acttcgcctt cgccgtttag ttcggcaatg
     8221 ccccggcgca tctccgggcc aatctggacc ttcgcaacat cgcgcagata agcgggcacg
     8281 ccattttcac tggcttttaa aacgatgtga ttaaagtcgt cgagcgtttg cagatagccg
     8341 ctggcgcgca ccatatattc cgcttccgcc agttcgatcg acgaaccgcc cgcttcctgg
     8401 tttgaagcat ccagcgcgct ttttacttcg gcgagactga tgccatactg cgccaggcgc
     8461 tggggatcga taaccacctg atactctttc accacaccgc ccaccgacgc cacttccgca
     8521 acgtcaggga tggttttcag ctcatatttg agaaaccagt cctgtaatga gcgtaaatcg
     8581 gccagatcgt gcttaccgct gcgatccacc agtgcatatt catagatcca gccaacaccc
     8641 gtggcatctg gccccagctc ggcgctgact cccgcaggct gcttaccctg tacctggttg
     8701 aggtactcca gcacccgcga acgcgcccag tacggatcgg taccatcttc gaaaatgaca
     8761 tacacataag agtcgccaaa ctgcgagaaa ccacgcacag tcttcgcgcc aggcaccgac
     8821 aacatggtgg tggttagcgg ataagtcacc tgattttcaa cgatttgcgg tgcctgaccg
     8881 ggatagctgg ttttaataat cacctgcaca tcggagagat ccggcagcgc atccactggc
     8941 gtgttaatga tggtccaggt gccccagata ctcagaaata acgcgcccat cagcaccaga
     9001 aaacggttcg ccaccgagcg acgaataatc cattcaatca ttggttattc cctcaatgcg
     9061 catgggtagc actttcagag cgcatccgct ccagtacgcc agaaatattg gcttcggaat
     9121 caatcaggaa caggccgctg gaaaccacct tttcaccttc cgccagacca gagcgtaatg
     9181 cggtgacgcc ttgcgatgcc tggaaaacag caacgcgttt cggtacaaag cgcccgtcgg
     9241 catcaacggt aatcacccgc tgttcgttgc cggtatcaat cagcgcctgt gacggaatga
     9301 gcagcatcgg ttcgctggcg gtgttgagtt gcaaccaggc gttcatgccc ggttttagcg
     9361 cctcgtcggc gttgtcgact tccagacgca gctgcagcgt gcgggtcgcg gcatccacgc
     9421 caggtagcag cgtccatttg cggatggtga gtgttttatc cggtcgcgcc ggaacggtga
     9481 gcgtaaactg cgaggcatct ttcaccagcc aggcgatgga ctccgggatc gcagcagtga
     9541 cccacaccgg gtccataccc tgaattttcg cgaccacgtt atctttggcg atattcattc
     9601 ccgcgcgcag atcaaacgcg gtgatcacgc catcaatggg cgctttgagc gtaaagcgag
     9661 tctggatttt ttgcgtggcg atcaggcggc gaatatccgc ctccggcatt cccgccagtc
     9721 gcagccgctc aagaatgcct tcagtctggg tcgccgtacc gccggtttcg cgcagcagta
     9781 aatactcact ctgcgcttcc acccagtcag gaatagtcag gtcgagaagc ggtgtgccct
     9841 tttgtacttt atcgcccacg gtaagcggat acaccttgtc gataaacccg gcagcgcgag
     9901 cctgcacaat ggcatactga tactcgttgt aactgacatt cgccgggaaa ctctgggcaa
     9961 aagtcagcgg tccgcgcgtg acggtagccg ttttcacccc cagattctgc gtctgagtcg
    10021 ggtcaatgcg cacaccagac gcagaactct cttcatcggc atatttcggc accagatcca
    10081 tatccataaa cggcgattta cctggtttat cgaaccgtgt attggggtac atcgggtcgt
    10141 accagaataa gattttacgt tctgcggtcg acgttttttc tgcgggtggt tcagcctttg
    10201 caaaccaggt aaaacctgcc gcagaaataa taccgcccgc gatcatgctg ccgataataa
    10261 gcgcgatttt tttcatctca ttaaacctgg gttactggct gactttaata tcctgtaata
    10321 aagaaaggtt gccttgctgg acaaaattaa acgccacttt gtcgccagtt ttaatttcac
    10381 tcattttcgt ctgcggagtg atggtaaagc gcatggtcat ctccggccag ttcacggcag
    10441 caatcggatc gtgatggatg gtgatttttt tgctttccag atcaataccc tttaccacgc
    10501 cagtggcgct aataacctgt ggttttgctt cgctcatggt ttcatgatga tgttcgttag
    10561 cctgggcatt aaagccaata acggtaaaca gactgaacat tgcgacttgc agtgcttttt
    10621 tcatttattc tctcctggag ttaaaaatta ttgctgccaa ccgcccccta aggcggtata
    10681 caaagaaatt tcgttaacct gacgggcata attcaggtcg agtaaagttt gtcgggttgc
    10741 aaataaagaa cgctcggcat ccagcacttc cagataactt accgcgccgt gctgatataa
    10801 tgcccacgcc cgttgcaaag taatttgcag cgacgccaga taacgctgct gggcgctgat
    10861 ttgatcgttc aggctttgac gtaatgccag cgcatctgcc acttctttaa aggcgttctg
    10921 gattttctgt tcataattca ctaccgactg ctgctggcga atttcggcga tatccagatt
    10981 ggcctggttg cgtccggcat taataaatgg ggatctcaat tttaggaata aaattccaca
    11041 tcccgctgct agcgttaaat aatgaagaca gatcgctgct ggcggttgat attccgctgg
    11101 tcaggctgat agacgggaaa aatgccgcac gcgcagcacc aatattagca ttagccgcca
    11161 ttaacgcgtg ttcagcttcc ataatatccg ggcgctgcaa taagatttgc gacgacaagc
    11221 ccgccggtaa tttaacgctt tgcaggctgc cgctgtttac tgtctgcgct tgcggcagtt
    11281 tgccgtagct tcccaataac agttgcaatg cattattcgc ctgcgccagt tccccctgac
    11341 gtttagcgat gtcactgcgg gtactttcta tcaccccgcg agcctgttcc agcgccagaa
    11401 cattgctgct accggtcaac agttgttttt cgacaaacgc atatgactgc tgataattac
    11461 gcagcgtttc ttcggctatt tgcaattgcg catacgccag ttgctgattg aaatagcttt
    11521 gcgcgacatt agaaaccagc agaatatgca ccgcgcgctg agcttcctca gtggctaaat
    11581 aattttgtcg ctcggcttcg ctcatgttct ttaagcgacc gaaaaaatcg aggtcaaagc
    11641 tggcgttaag gccggtcgaa aactcccgtg tcgtggcggt attcccttta agattaccgc
    11701 tccagctgcc gctgccctcg ccattgagct gtgggtagcg gtcggcatcg gtcagacgat
    11761 attgcgcccg tgcctcctgc actttcagcg tcgccatgcg caaatcccgg ttattcacca
    11821 gcgcctcgct aatcagcgtc ttcacctgat tatcaacaaa aaaggtgcgc cagcccacgt
    11881 tctggtagtt atctgctgcg ttaaccaagc cgttctggct gagtgagaac tgctgcggca
    11941 cgggcattgc cggacgctga taatccggtg ccagtgaaca accggttagc gcaagggcca
    12001 cacaaaatgg cagaagttta caaggagaca taggctcata atttctggtg attttatacc
    12061 gccaacttta ctcgccaggc tctgattttc cggtgacagg aaaatgacaa aattgtcatt
    12121 ttgccaataa gcgattgcca tctgatcccg ctactctaga attgcccggg caacatgcgg
    12181 aggaaaaatg aaactgttga ttgtcgaaga tgaaaagaaa accggagaat acttgaccaa
    12241 agggttaacc gaagccggtt ttgtggtcga tttggccgac aacgggctga atggctacca
    12301 tctggcgatg accggtgatt atgacctgat aatcctcgat attatgctgc cggacgtgaa
    12361 cggctgggat atcgtgcgca tgttgcgctc cgccaataaa gggatgccga ttctgttgct
    12421 taccgcgctt ggcaccattg aacatcgcgt caaggggctg gagttggggg ccgatgacta
    12481 tctggtgaag ccgttcgctt ttgctgaact gctggcgcgg gtgcgcactc tcctgcggcg
    12541 cggggcggcg gtgattatcg aaagtcagtt tcaggttgcc gatctgatgg tcgatctcgt
    12601 cagccgcaaa gtcacccgca gcggcacgcg catcactctg accagtaaag agtttactct
    12661 gctggagttt tttctccgcc atcagggcga agtgctgccc cgctcgctta tcgcctcgca
    12721 ggtgtgggac atgaatttcg acagcgacac taacgccatt gatgtggcgg tgaagcggct
    12781 acgcggcaaa atcgacaacg actttgagcc gaagctgatt cagaccgtgc gcggcgtggg
    12841 ctatatgctt gaggtgccgg atggtcagta agccatttca gcgcccgttt tcgctggcaa
    12901 cccgtctgac cttttttatc agcctggcca ccatcgcggc gtttttcgcc tttgcatgga
    12961 tcatgatcca ctcagtgaaa gtgcattttg ccgagcagga tattaatgat ttaaaagaaa
    13021 ttagcgccac ccttgaacgg gtacttaatc atcctgacga gacgcaagcc cgacgcttaa
    13081 tgacgctgga agatatcgtc agtggttatt ccaacgtgtt gatttccctg gcagatagcc
    13141 acggcaaaac ggtgtatcac tcccccggtg cgccggatat ccgcgagttt acgcgtgacg
    13201 ccatacccga taaagacgcg cggggcggtg aggtgtatct cctttccggc ccgacgatga
    13261 tgatgccagg ccacggtcac gggcatatgg aacacagcaa ctggcggatg attaacttac
    13321 cggttggccc gctggttgac ggcaaaccga tttatacgct ctacatcgcg ctttcgatcg
    13381 attttcatct tcattacata aatgatttga tgaataaact tattatgacc gcatctgtca
    13441 tcagcatcct gatcgtcttt atcgtgctat tggcggtaca taaaggtcac gcgccgatcc
    13501 gcagcgtcag ccgtcaaatc cagaatatca cctcgaaaga tctcgacgtt cgccttgacc
    13561 cgcagaccgt gcccattgag ctggaacagc tggtactgtc gttcaaccat atgatcgagc
    13621 gtattgagga tgtctttacc cgccagtcca atttctcagc ggatatcgct cacgaaattc
    13681 gcacgccgat taccaatctc atcacgcaaa cggaaatcgc cctcagccag tcgcgcagcc
    13741 agaaggaact ggaagatgtg ctctactcta atctcgaaga gctgacgcga atggcgaaaa
    13801 tggtcagcga tatgctgttt ctcgctcagg ccgataacaa ccagctaatc cccgaaaaga
    13861 aaatgctcaa cctggcggat gaagtcagca aagtgttcga ttttttcgag gcgttagcgg
    13921 aagatcgcgg cgtggagttg cggtttgttg gcgacaaatg tcaggttgcg ggcgatccgc
    13981 tgatgctgcg tcgggcgtta agcaacctgc tttctaatgc cctgcgttat acgccaccca
    14041 gtgaggctat tgtagtgcgc tgccagacgg tcaatcatca ggtgcaagtt tccgtcgaaa
    14101 accccggtac gcccattgcg cccgagcact taccgcgatt gtttgaccgt ttctatcgcg
    14161 ttgacccttc ccgccagcga aaaggtgaag gtagcggtat tgggctggcg atagtgaaat
    14221 cgattgttgt cgcgctaaag gcacggttgc ggtaacgtca gatgcgcggg ggacaaggtt
    14281 tgtgattatg ctgccggaga gagagtgatt attaagttca aattcagggc gtggtgcatc
    14341 acctcctggt attgttgatc aacaaattag agaaaaactt attaaacata tgaaatatcg
    14401 aaggctttta tttttcaaag tttcacgaga tgtttgtata tgaaacttag ttaaatctgt
    14461 gtttcttcca attgtagatc acaatgccct ttctaaattt tcatcattat a
//
LOCUS       DAZKHC020000102        14474 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZKHC020000102 DAZKHC020000000
VERSION     DAZKHC020000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.102"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            26..591
                     /locus_tag="ACHOSM_RS18805"
                     /old_locus_tag="pgaptmp_003761"
                     /pseudo
     CDS             26..591
                     /locus_tag="ACHOSM_RS18805"
                     /old_locus_tag="pgaptmp_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            642..3101
                     /locus_tag="ACHOSM_RS18810"
                     /old_locus_tag="pgaptmp_003762"
     CDS             642..3101
                     /locus_tag="ACHOSM_RS18810"
                     /old_locus_tag="pgaptmp_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4560178.1"
                     /translation="MDTVNIYRLSFVSCLVMAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINHCVDFSSRPEMLFNFDQASQQLNITIPQAWLAWHSENWTPPSTWKEGVAGIL
                     MDYNLFASSYRPQDGSSSTNLNAYGTTGINAGAWRLRSDYQLNQTDSDDNHEQSGGIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDERMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDHDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIKGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVRGDNQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3116..3847
                     /locus_tag="ACHOSM_RS18815"
                     /old_locus_tag="pgaptmp_003763"
     CDS             3116..3847
                     /locus_tag="ACHOSM_RS18815"
                     /old_locus_tag="pgaptmp_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4560179.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3844..4905
                     /locus_tag="ACHOSM_RS18820"
                     /old_locus_tag="pgaptmp_003764"
     CDS             3844..4905
                     /locus_tag="ACHOSM_RS18820"
                     /old_locus_tag="pgaptmp_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4560180.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFVKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADLTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5057..6103
                     /locus_tag="ACHOSM_RS18825"
                     /old_locus_tag="pgaptmp_003765"
     CDS             5057..6103
                     /locus_tag="ACHOSM_RS18825"
                     /old_locus_tag="pgaptmp_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGU4560181.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6100..6891)
                     /gene="nei"
                     /locus_tag="ACHOSM_RS18830"
                     /old_locus_tag="pgaptmp_003766"
     CDS             complement(6100..6891)
                     /gene="nei"
                     /locus_tag="ACHOSM_RS18830"
                     /old_locus_tag="pgaptmp_003766"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HGU4560182.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6927..7661)
                     /gene="pxpA"
                     /locus_tag="ACHOSM_RS18835"
                     /old_locus_tag="pgaptmp_003767"
     CDS             complement(6927..7661)
                     /gene="pxpA"
                     /locus_tag="ACHOSM_RS18835"
                     /old_locus_tag="pgaptmp_003767"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HGU4560183.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7651..8582)
                     /gene="pxpC"
                     /locus_tag="ACHOSM_RS18840"
                     /old_locus_tag="pgaptmp_003768"
                     /pseudo
     CDS             complement(7651..8582)
                     /gene="pxpC"
                     /locus_tag="ACHOSM_RS18840"
                     /old_locus_tag="pgaptmp_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(8576..9232)
                     /gene="pxpB"
                     /locus_tag="ACHOSM_RS18845"
                     /old_locus_tag="pgaptmp_003769"
     CDS             complement(8576..9232)
                     /gene="pxpB"
                     /locus_tag="ACHOSM_RS18845"
                     /old_locus_tag="pgaptmp_003769"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HGU4560184.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9255..9998)
                     /gene="ybgI"
                     /locus_tag="ACHOSM_RS18850"
                     /old_locus_tag="pgaptmp_003770"
     CDS             complement(9255..9998)
                     /gene="ybgI"
                     /locus_tag="ACHOSM_RS18850"
                     /old_locus_tag="pgaptmp_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HGU4560185.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10269..11750
                     /gene="dtpD"
                     /locus_tag="ACHOSM_RS18855"
                     /old_locus_tag="pgaptmp_003771"
     CDS             10269..11750
                     /gene="dtpD"
                     /locus_tag="ACHOSM_RS18855"
                     /old_locus_tag="pgaptmp_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HGU4560186.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLIVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11792..13210)
                     /gene="phrB"
                     /locus_tag="ACHOSM_RS18860"
                     /old_locus_tag="pgaptmp_003772"
     CDS             complement(11792..13210)
                     /gene="phrB"
                     /locus_tag="ACHOSM_RS18860"
                     /old_locus_tag="pgaptmp_003772"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HGU4560187.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFAASVEIIKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVLCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13207..13716)
                     /locus_tag="ACHOSM_RS18865"
                     /old_locus_tag="pgaptmp_003773"
     CDS             complement(13207..13716)
                     /locus_tag="ACHOSM_RS18865"
                     /old_locus_tag="pgaptmp_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HGU4560188.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13867..14474)
                     /locus_tag="ACHOSM_RS18870"
                     /old_locus_tag="pgaptmp_003774"
                     /pseudo
     CDS             complement(13867..14474)
                     /locus_tag="ACHOSM_RS18870"
                     /old_locus_tag="pgaptmp_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3502 a         3866 c         3505 g         3601 t
ORIGIN      
        1 ttcttttctg ttaaaggact cagttatgtt taaaggacaa aaaacattgg ccgcactggc
       61 cgtatctctg ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca
      121 ctttaaggtg aagttattga agcaccgtgt gaaatacatc aggatgatat tgataaagag
      181 gttgaactcg gtcaggtgac caccagccac attaatcagt cacatcacag cgatgccgtt
      241 gctgtcgacc tgcgcttagt caactgtgat ctggaaaact ccagcaacgg ttccggtggc
      301 aagatttcta aagttgcagt cacctttgat agctcagcga aaaccaccgg cgcagatccg
      361 attctcaaca acaccagcac gggtgaagcc accggcatcg gcgtacgttt aatgaataaa
      421 gatcaaagca atatcgtttt aggcaccgct actccagata tcgacctggc accgacctcc
      481 agcgaacaaa cgctgaattt ctttgcctgg atggaacaaa ttgatcaggc tacacctgta
      541 acgccaggcg cagttacagc gaatgctacc tacgtgctgg attataaata agtattactt
      601 tggcggcgct atcgccgcct ttcttcaaat atttatcagg tatggacacc gtgaatattt
      661 atcgactctc ttttgtatcc tgcctggtta tggcgatgcc ttgcgcattg gcggtcgaat
      721 tcaaccttaa tgttctcgat aaatcgatgc gcgaccgcat tgatatttca ttattaaagg
      781 aaaaaggagt cattgctccc ggtgagtatt ttgttagcgt tgcggtaaat aataaccaaa
      841 tcagtaacgg gcaaaagatt aactggcaca aaaatgacga taaaaccatt ccgtgcatca
      901 atgatttact ggtcgataaa tttggcttaa aacctgaagt ccgtcagtcg ttaccattga
      961 taaatcactg cgtcgatttt agctcccgac ctgaaatgct cttcaatttt gatcaggcca
     1021 gtcagcaact aaatatcacc attccgcagg cctggctggc gtggcactca gaaaactgga
     1081 ccccaccctc cacatggaaa gaaggtgtcg ccggtatcct gatggattac aacttgtttg
     1141 ccagcagcta ccgcccacag gacggcagca gcagcactaa cctgaacgcc tacggtacca
     1201 ccggaattaa cgccggagca tggcgcttac gtagtgatta tcagttgaat cagactgata
     1261 gcgatgataa ccatgaacag tcaggcggaa tatcgcgcac ctatcttttt cgtccattac
     1321 cgcaattagg ctctaaatta accctcggcg aaacggattt tagttccaat attttcgacg
     1381 gtttttctta taccggcgcg gcactggcaa gtgacgagcg aatgttgcca tgggaactac
     1441 gcggctacgc cccacaaatt agcggtattg cacagaccaa tgccacggtg acgatcagtc
     1501 aatcaggccg cgtcatttac cagaaaaaag tcccaccagg cccatttatc attgacgacc
     1561 ttaatcagtc tgttcagggc acactggatg tcaaagtgac ggaagaagat ggtcgggtga
     1621 acaatttcca ggtttcggca gcatcgacgc ccttcctgac tcgtcaggga caggttcgct
     1681 ataaactggc cgcgggtcag ccacggccct ccatgtcaca tcaaactgaa aatgaaacct
     1741 tttttagcaa tgaagtttcc tgggggatgc tgtcaaacac ctcgctgtac ggcggcctgc
     1801 tgctttctgg tgatgactac cattctgccg caatgggtat tgggcaaaat atgctgtggc
     1861 ttggtgcgct gtcgtttgat gtcacgtggg ccagtagcca ttttgatact cagcaggacg
     1921 agcggggctt aagctaccgt tttaattaca gcaaacaagt ggatgccact aacagcacga
     1981 tttccctcgc cgcttatcgt ttctccgatc gtcattttca cagctacgcc aactatctgg
     2041 atcacaaata caacgacagc gatgcgcagg acgaaaaaca gacgatcagc ttatctgtgg
     2101 gccaaccgat taccccacta aacctcaatc tttacgccaa cctgctacat caaacctggt
     2161 ggaatgcaga cgcctccacg accgccaaca tcacggcagg ttttaatgtt gatattggtg
     2221 actggagaga tatctctatt tcgacgtcat tcaatacaac ccattacgaa gataaagatc
     2281 acgacaacca gatttacctg tcgatttcgc tccccttcgg taacggtggt cgggttggct
     2341 atgacatgca aaacagtagc cacagcacca cacaccgcat gtcgtggaac gatacgctgg
     2401 atgaacgtaa tagctggggc atgtctgccg gactgcaatc cgaccgtcct gacaatggag
     2461 cccaggtgag cggtaactat cagcacctga gttcagcggg tgagtgggat atttctggta
     2521 cctatgccgc caatgattac agttccgtca gcagcagctg gagcggttct ttcaccgcaa
     2581 cccaatatgg tgcagcgttt catcgccgca gctccaccaa tgaaccacgc ctgatggtca
     2641 gcaccgatgg cgtggcagat attccggttc agggcaatct cgactacacc aaccattttg
     2701 gcattgcggt ggtgccgttg atttccagtt accagccttc caccgtggcg gtgaacatga
     2761 atgacttacc cgacggcgta acagttgcag aaaacgttat caaagaaacg tggattaaag
     2821 gcgcgatagg ttacaaatca ctggcttccc gttccggtaa agacgttaac gtcatcattc
     2881 gcaacgccag cggtcagttt cctcccctcg gagcggatat ccgccaggat gacagcggca
     2941 ttagcgtggg gatggttggc gaggaaggac atgcctggtt aagcggagtc gctgaaaatc
     3001 aaaagtttac cgtggtccgg ggtgataacc agcattgctc gctccatctt cctgaacata
     3061 tggaagacac cgcaaatcgc ctgattttac cttgtcatta attaaggaaa tgaaaatgac
     3121 atttatgaaa ggactacccc ttttgttgtt ggttgccagt ctgtgcagcc acgctgcact
     3181 acaacccgat cgcactcgta ttgtgtttaa cgctaatgat aaagctacca gtctgcgggt
     3241 ggataatcgt agcgataaac tcccctacct cgcttattcc tggctcgaaa atgaaaaagg
     3301 ggagaaaagc gatgatcttc tggtcgccct gccgccgata caacgtctgg aaccgaaagc
     3361 aacaacgcag gtacggatag taaagcaagc ctcgaccaca aaattaccgg gcgatcgcga
     3421 aacgctattt ttctacaata tgcgtgaaat tcctcccgcc ccggaaaaaa acagcgacca
     3481 tgccgttctg caagtcgcca ttcagagtcg tatcaaggtg ttctggcgac cggctgcatt
     3541 acgcaagaaa gcaggagaaa aggtcgaact gcagttacag gtcagccagc agggcaacca
     3601 actgacactg aaaaatccta ccgcgtatta tctgaccatt gcctacctgg gacgaaatga
     3661 aaaaggcgtt ctccccggct tcaaaaccgt gatggttgcg ccttttagta cggtcaacac
     3721 aaacaccgga aattatagcg gcagccagtt ttatctcggt tatatggatg attacggcgc
     3781 gttacgcatg acgacgctca actgcagcgg acaatgccat ttacaagccg tggaggcgaa
     3841 gaaatgagtg ctggcaaggg agtgttgctc gtcatctgtc tgttatttct gccgttgaag
     3901 tctgcgcttg ccctgaactg ctactttggt tcatccggtg gctcggtaga aaaatcagaa
     3961 gcgattcaac cttttgctgt accaggcaat gccaaactcg gcgataagat ttgggaatct
     4021 gacgatatta aaattcccgt ctattgtgac aacaatacca acggcaattt tgaaagtgag
     4081 catgtttatg cctgggtaaa cccctacccg ggggtgcagg atcgctatta tcaactcggc
     4141 gtaacttaca atggcgttga ttatgacgcc aaccagggaa aaagccgcat cgacactaac
     4201 cagtgtattg acagtaagaa cattgacatt tatacccctg agcagattat cgcgatggga
     4261 tggcagaaca aaatttgctc gggtgatccc gctaatattc acatgtcacg tacctttctc
     4321 gctcgcatgc ggttatacgt caaaatacga gaaatgccgc cgcatgatta tcaaagtacg
     4381 cttagcgact atatcgtcgt gcaatttgac ggtgccggta gcgttaacga agatcctact
     4441 gcccaaaacc tgaaatatca tattaccggt ctggaaaaca ttcgcgtgct ggattgcagc
     4501 gtcaattttt ccatttcacc ggaaacacag gtgattgatt ttgttaaatt taatttgctg
     4561 gatattcgtc gccacacaat gtcgaaaaca ttcagtatta agacgacaaa atcacaaaat
     4621 gaccaatgca ccgacggatt taaagtcagt tcctcctttt ataccgaaga aacgttggtt
     4681 gaagaagata aagcattgtt gattggtaat ggtctgaagc tacgtttgct ggatgaaaac
     4741 gcctcgcctt ataccttcaa taaatatgct gaatacgccg atctcaccag cgacatgttg
     4801 gtctacgaaa aaacctatac agctgaactt tcgtccattg caggtacccc catcgaggct
     4861 gggcccttcg atacagtggt gctttttaaa attaactata actgatttgc catgatgccc
     4921 tttgcagccg gaaagggcat ctctttcaga aaattgctga ttttcccctt ttccttgatt
     4981 gctgttattt tttcctggcc cggctttctt tatgctgtat cgatacacca aagagaaaat
     5041 aatgagggag cgaaggatgc cagttttgca gtggggaatg ttatgtgtgc tatcacttct
     5101 cctttctatt ggtttcctcg cggtacatct cccggcggcg ctattacttg ggccgatgat
     5161 cgccgggatc atcttcagta tgcgcggaat tactctgcaa cttccccgct ccgcttttct
     5221 cgccgcgcag gccattcttg gctgcatgat tgcgcaaaac ctcactggtt cgattctcac
     5281 cacgctggcc gccaactggc cgatcgtgct tgctatttta ctggtgacgc tgctttccag
     5341 cgccatcgtg ggttggttat tggtgcgcta tagctcactg cctggaaata ccggtgcctg
     5401 gggttcttct ccaggcggcg cagcggcaat ggtcgctatg gcgcaagatt acggcgcaga
     5461 tattcgcctg gtggcgttta tgcaatatct gcgagtgctg ttcgtcgcgg gggccgctgt
     5521 tctggtcacc cgcatgatgc tgggtgataa cgctgaagcg gttaaccagc aaatcgtctg
     5581 gttcccgcca gtgagcatta atctcctgct tacgattttg ctcgcagtcg ttgccggtac
     5641 ggcgggatgc atgctgcgtc ttccttccgg cacgatgctc atcccgatgc tggcgggcgc
     5701 agtgctccag tctggtcagc tcatcaccat cgaactaccg gaatggctgc tggcgatggc
     5761 gtatatggca attggctggc ggattggtct tggtttcgat aaacaaatct tactgcgggc
     5821 actacgccca ctaccgcaaa tcctgctgtc gatttttgct ctgctggcta tttgtgcagg
     5881 tatggcgtgg ggactgaccc ggtttatgca tattgatttt atgactgcct acctcgccac
     5941 cagtcccggc gggcttgata ccgtggcggt catcgccgca gggagcaatg ccgatatggc
     6001 gcttattatg gcgatgcaaa ccctgcgcct gttcagtatt ttgctgacgg ggccagccat
     6061 tgcgcggttt atttcaacct atgcgccgaa gcggtcggcc tagtgctggc agcctggaca
     6121 ccagtaaaac gggcgcgagg acaacgtggt tttctcaatg atgccgccac agcgttcgca
     6181 cagttcgcca tcacgatgaa aaaccttaaa gcgaaacagc gccccatgat gcttattctc
     6241 atccacctgc ccccgcgtag cgtaagaaaa ccgaggaatc tccagtaacg cgtgtgcgag
     6301 tgcatccagt tgcgccgcat tgagatcttt cgctttatga tttccagtca acccaacctg
     6361 ccagaggatc tccacccgca aataattgcc aagcccagcc agaaacgcct gatcgagcag
     6421 taatcctgca aactgacggt tacgaaagcg cggtgacaat aaccgttcct ttaccacctc
     6481 cggcgtcaga ttcggatcca gcacatcggg accaacgcgt tgtaaaaacg gatgcgtggt
     6541 cagttgttcc ggcctcaaca tctcaatatc cgaggcgcta taaagcagaa tggttttgtc
     6601 agccgtttgc agttttaccc gcaatactcg cgtggtctgc ggctcttcgc cggtatcaac
     6661 cacgcgccag acgccgtaaa gctgattatg gctgtagagc gttaagtcgt tggaaaaatg
     6721 agttaacaac gccttaccac gcgtttccac atgggtaacg tgttgaccga taagttgtga
     6781 ttgataagtt tttaactgcg ggaaggcaaa ccagacatca gttagtggtt tgcctttgat
     6841 cgccgcctcc aggttatccg ctgcacggcg gatctccggg ccttcaggca tggtgatatc
     6901 cttttttcat tctttattaa tcggggttat gctgcgacaa caatctcctt ttcggcaaat
     6961 gtagagcgta gtcggcgggc gaacgccagt gcgtgctcgc cgtcgccatg caggcagact
     7021 gtttgcgccg tgaccgttgc ccattcgccg gtgatacttt tgactctgcc gtgttgcacc
     7081 atctctagcg tttgcgccag cgcctgttct tcgttttcaa tcaacgcgcc tggctgactt
     7141 cgcggcacca gcgagccgtc agcctgataa ccgcgatcgg caaacacttc ctcgcgcgtt
     7201 gtcaggccat attgcttgcc tgcacgaatc agctcgcttc ctgccagccc aacgagaacc
     7261 aatgctggat cgcaagcgta taccgctctg gcgatggcgt ctgccagttg tgcttctttc
     7321 gccgcctggt tgtacaacat gccgtgcggt ttgacatgac gcattacgcc gccttgcgca
     7381 cgggcaatcg ttgccagcgc gccaatttga tacagcgtct gggcgtaaac ggtttctggc
     7441 ggcagctgca tggcgctgcg accaaaattt tccctgtcgg gaaaactcgg gtgagcgcca
     7501 atcgcgacac cattttttat tgcttcacgc acgcaagcct gcatggtttg ggcatcgcct
     7561 gcatgaaatc cacaagcaat attggcagag gaaaccagcg ttaatagctc tgcgtcgctg
     7621 gcgcagcctt cgcccagatc ggcgttcagg tcaattttca ttgtgcagcc gccacgctaa
     7681 ttgttcaaaa taacgttgct gatcttgccg cgctttcagt gcctcttcca gtgaacactg
     7741 gacaaaatga atcggctgac cgagcggaat ttgcgccaga tggtacatat cagcctcaat
     7801 gatacaggca atacgcgggt aaccaccggt ggtctgtgcg tcgttcatca acacaatcag
     7861 ctgcccgtta tgtggcacct gcaccacgcc cggtaacaaa ccgtgagata acagttcgcg
     7921 atcggtggtg cgttttaaaa tttgcccctg taagcgatag cccatgcggt tactttgcga
     7981 gctaagctgc cagggcgaac gccagaatgc atcctgcgag gcgcgatcga actcatgata
     8041 ttccggcccc ggcaaggcgc gaatgcggtt gccccacagc agctgtttaa cgccctgcgc
     8101 ttccataaaa tcacgcttcg atttgccaat cgggagtcgg tcaccatcct tcagtaaacg
     8161 gccttccagc ccgccaatcc ccactttgag atcggtgctg catgacccta ttaccggcgg
     8221 aacatcaata ccacccgcga ccgccagata actgcgcatc ccgtgctgcg ggcgtttaag
     8281 cgttaaacgc tggctgcttt catcggcaat cgccagccgg tccagacggc attatcatcc
     8341 agccgcgctt cgcaaccggc acccgtcaga gcaaaccacc catcagtttc gaactcaacc
     8401 gttaactgac cgagcgtgat ctccagcgcg ggggcattgg cgtcattacc caccagcagg
     8461 ttagcaatgc gtaacgcagg catatccagt gcgccgcagt ggctgattcc cgactggcga
     8521 aaaccgtgac gaccgccatc ctgcacagtg gtatacatgc ccgcacgaat aatcttcagc
     8581 atactccctc cttctgcggt acaaagcgca cgctgtctcc cggacgtaat aagatgggtt
     8641 cgtcacgcgc cggatcaaac aggctgagtg aggtatgacc aatcaactgc cagccacccg
     8701 gcgttgccag cggataaaca ccagtctgcg gcccgccgat cccgacagaa cctgccggaa
     8761 cgagcaagcg cggttcagcg cgccgtggcg tgtgtagttg ttccggcaaa ctcccgagat
     8821 acgggaagcc cggttgaaaa cctaaaaacc agaccacgta ttccacggag gagtgcaatt
     8881 caacaacctg tttttcgcta aacccgcaat gcgccgcgac caccgccaaa tccggtccgc
     8941 ctgcaccacc gtaaaccacc ggaatttcaa taaagcgaga ctcaggttcc agggcctcgc
     9001 tctcctccca ccagcgttgc aaacgctcta tggcatccag cgccagcgac tcaggattac
     9061 gcaaaatcac cgtgatattg ttcatgccgg gaatggcttc aaccacattc ggcatatcca
     9121 ccagacgctg cgccagtcgc cagatccgtt tctggctagc cagcgtcacc ggcggttcca
     9181 gttccagcac taccgccgtt tcacctatca gataacaacg cgctcgttgc actttcgatc
     9241 cctctgatta ttcgttatgc aggattagga atatcaataa aggtcacatc aagatcggta
     9301 ttttcattca gccactcgct caatgcgcga ataccaccac gttcagtggc atggtgacct
     9361 gcagcataaa aatgcaaccc ctgctcgcgg gctgaatgaa tggtctgttc agaaacttcg
     9421 ccagtaataa aagcatccac gccaaaacgc gcggcgctat cgataaaact ttgcccgccg
     9481 cccgtgcacc aggcgacgcg ctggaccacc tcaggtccgg tatcgccaca ccataacggc
     9541 ttacgtccca gacgcgcttc aatccaggag gctagttcca gtcccggcac aggcatggtc
     9601 agctctcccc acggcaccaa cggctcaatt tcgcccatga ccgtgatccc cagtaacgcc
     9661 gccagctgtg cgttattgcc cagctcagga tgtgcgtcaa gcggcaaatg ccagccatac
     9721 aggttgatat cattcgccag caacgttttt aaacggttac gcttcattcc gcgaatgacc
     9781 ggagactctc ctttccagaa gtagccatga tgcacaatga ccgcatccgc gcccagacgc
     9841 actgcctcat cgagcaaagc ctggctggcg gtgacaccgg taacaatttt ttgcaccgtc
     9901 tctttgcctt ccacctgcaa accattcggc gcgtaatcac taatcgccgc gctgttcagt
     9961 ttttcgttaa tcagttgttc cagttcggtg tttttcatct tcgcgccttc cgtattctga
    10021 tagctacacc gttaacatag cgttacctcg cctgttcgtc gatagccaat cttttgacat
    10081 attaagaaac aaacccgacg ccaaaagacg caaattgttt atatcatcac gcttcagcta
    10141 atgtatattt atgcaatcag acagcatatt gcataaataa tcagatctgt atcaaagttt
    10201 taactttaag gaaacacaag cacgccgcgc aactattacg tgacgttgcc gtcaattgtg
    10261 gaattattat gaataaacac gcatcacaac cgcgcgctat ttactatgtc gttgcgctgc
    10321 aaatctggga atattttagc ttttacggca tgcgtgccct gctgattctc tatctcacca
    10381 atcaactaaa atacaacgat actcacgcct acgagttatt tagcgcctac tgttcgctgg
    10441 tgtatgtcac gccaatcctc ggtggctttt tggcggataa agttctcggc aatcgcatgg
    10501 cggtgatgct gggggcgttg ttgatggcga ttggtcatgt ggtgctgggt gcaagtgaga
    10561 tccatccgtc attcctctat ctgtccctgg cgattatcgt ctgcggctat ggcctgttta
    10621 aatctaacgt aagctgtctg ctcggcgagc tgtatgagcc aaccgatccg cgtcgtgatg
    10681 gcggtttctc gctgatgtat gcggcgggta acgtggggtc tattatcgca cctatcgcct
    10741 gtggttacgc ccaggaagag tacagctggg cgatgggctt tggcctggcg gcggttggca
    10801 tgatcgcggg tctggtcatt ttcttatgtg gcaatcgtca tttcactcat acccgcggcg
    10861 ttaacaaaaa agtactgcgt gcgacaaact ttctcctgcc gaactgggga tggctgctgg
    10921 ttctgctggt ggcaacgcct gcactgatta ccgtactgtt ctggaaagag tggtcggtat
    10981 acgccttaat tgtcgcgact attattggcc tgggtgtact ggcaaaaatt tatcgcaaag
    11041 cagaaaacca gaaacagcgg aaggagctgg gactgattgt gacgctcacc ttcttcagta
    11101 tgttgttctg ggccttcgca caacagggcg gtagctcgat tagcctttat atcgaccgct
    11161 tcgttaaccg cgatatgttt ggttataccg ttccgaccgc gatgttccag tcgattaatg
    11221 ccttcgcagt tatgctgtgc ggtgtgttcc tggcgtgggt ggttaaagaa agcgtcgcag
    11281 gtaatcgtac cgtgcgcatc tggggaaaat ttgctcttgg ccttggcctg atgagcgccg
    11341 gattctgcat tctgacctta agcgcccgct ggtccgcaat gtatggtcac tcttctctgc
    11401 cactgatagt attaggcctg gcggtgatgg gctttgcgga actgtttatc gacccggttg
    11461 ccatgtcgca aattacgcgc attgaaattc cgggcgtgac cggcgtatta accggcatct
    11521 acatgctgct ttctggcgcg atcgcgaact atctggcggg cgtgattgcc gatcagacat
    11581 cgcaggcttc gtttgatgct tccggggcga tcaactactc catcaatgca tatattgaag
    11641 tatttgatca aattacctgg ggcgcactgg cgtgtgtagg actggtactg atgatttggc
    11701 tgtatcaggc gctgaaattc agaaaccgcg cgctggcgct ggagtcataa taggccagca
    11761 ccatacctga taaaacgctc gctgcgtttt atcaggctcc tttcctcgct tcttcgtacg
    11821 ctgccagcgt tcgcagcctt gcctctttgt gatcaactat cggttgcgga taatccagct
    11881 tcacacctgc tttctgcgcc cacttccacg gctcatgcac cgctttccct ggcacatcgc
    11941 gcagctccgg taaccatcga cgaataaact cgccctcacg gtcaaatttc tcgccctgag
    12001 ttatcggatt gaaaatacga aaatacggcg cagcatcagt acccgtagaa gccgcccact
    12061 gccagccacc gttattagct gccaaatcac catcaatcag ctgcgacatg aaatatcgct
    12121 cgccttcgcg ccagtcgatc aacaaatctt tcaccagaaa actggctgta atcatccgta
    12181 gccggttatg catccagcca gtgctgttaa gctgacgcat agcggcatca acaatcgggt
    12241 atcccgtttt gcctttctgc caggcctgta aatgtgcggg attgctttgc cactgcacac
    12301 gatccgtcca ggcaataaat ggacgatgtt tacacaacga ggggtaatac gtcatcagat
    12361 gacggtagaa ctcgcgccag atcagctcac taagccagac actaccggcc ccaccgtcca
    12421 gcgcctgcgg ctgttcagcc aacaagcgat gcaagcactg gcgaggcgat aacccgcccg
    12481 ttgccaggct ggccgacaaa cggctggtgc cttccactgc cggaaaatct cgttgttgct
    12541 catattctcc ggcaccgttc tggcaaaact ggcgtaattg cgcaatcgcc gctttttctt
    12601 ccaccggaaa atgcgcagta tcgaaagact gacgaggata attcagcgta atggatggcg
    12661 agggctctat cgatccgcta ctacgaactt ttggcgcagc gacgcactcc ggcatccctt
    12721 cccgcagccg tttcagccag gcattcttaa aaggcgtaaa gactttgtac atctcgtgat
    12781 tgccggtcat caccgcgcca ggcggcagga tcacgctgtc atcaaaccct tcgcacaaca
    12841 cgttacgcag cgctctttca acgtccacat cccgcgcccg ctcattcacc tcatactgat
    12901 agttataaaa aagatgagta acgctgtttt ccgcgcacac ctgtttaatt atttcgacgc
    12961 tggcggcaaa gtcatccact tcacggaaca ataaaggaat acctttttcc gcaagcgcta
    13021 tttgtagccc attcagttga gcattgataa gttcagcctg acgcggcgac atgttatgcg
    13081 tcgcccactg gcgtggtgta gcgatatata acgccagcac gcgtgcagac gaattgcggc
    13141 aggcggcagc cagtgcgaga ttatcgtgca gacgtaaatc ctggcgaaac cagaccagat
    13201 gagtagtcat aaaactcctg aatggcgcaa gttaatcaca cgcggccaaa gttcgaaata
    13261 acgctgccct gaaagccagg cgtcaggata aagcgccata tagtgtttga tacgcatcag
    13321 cggcgcaaga agtggttgct cgccacggcg ataactgtcg ataagcgccg ccaactgctg
    13381 gcgttctgtg gaatcaatat gcgggcgaaa ataaccctga acgtgcatca aaacgttggt
    13441 gtgatcgcgc gggttggcgg gatgagaaag caaaacaatt acgcgttggt agtactggtt
    13501 gtaaaaatca tccagatttt gccactggtg aatatcagcc acgaacgggc caagtttgcg
    13561 gtattccggc tgggaatgcg ccagaaagac gagtttatag cggctgtgat aatcgagaag
    13621 cgccccgcgc gttaagccat gttctttcag tcgatgtaac tcatgaaggg cgaagatacg
    13681 gattagggtg ctgtcatcaa atcgttgctg attcattact gctcctgaac ggtttacagg
    13741 agtaagtgta gacgttgaga aatgatattt gtgaagttaa tcctgttgta gcaggacaaa
    13801 tttacgaaag cctgctcccc gcaaggactg acgtcaggta gtttctaacc atagctgccc
    13861 tttctattca aacgtgaaat caataagctc atcagggaca tcgcaaacaa tatgaagacg
    13921 ggtttcttct ctgccgtgac tcttttcact aattgcgtaa ctgtcatgct tgggattatt
    13981 taattctttc agcggaaatt tttcctcgaa ggctttattt agccgcccct ggtttccttt
    14041 tacagcgaat aaataatcac ctccctgttt ttgtatcttc tctgcaatat ctttctggca
    14101 acccatcgca tcggttgtga tgatttttcc tttaatatcc agcatgttaa gaagttcagg
    14161 gatagctgtg atctcattgg atttcttatc cgtcttgatc tgcccgatga ccagactgtg
    14221 cattgttaag aacgcactaa tgacatgaat cgctccctta tggcgactct tgtcataaga
    14281 atgccggtgt atttttccat caattgcgat gacgtcttta tcatttgatg aatggcaatc
    14341 acgcatccag ttaataaagc actcgtgaaa ttttgcagga ctgatacagg atacaactct
    14401 ggcaatggta tcgtggacag gaataccatt ttcaaaatca ccatattgct tcaaaaaatc
    14461 gagatgtgtt tccc
//
LOCUS       DAZKHC020000103        14101 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZKHC020000103 DAZKHC020000000
VERSION     DAZKHC020000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.103"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            165..464
                     /gene="yoaC"
                     /locus_tag="ACHOSM_RS18875"
                     /old_locus_tag="pgaptmp_003775"
     CDS             165..464
                     /gene="yoaC"
                     /locus_tag="ACHOSM_RS18875"
                     /old_locus_tag="pgaptmp_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HGU4560189.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(584..763)
                     /gene="yoaH"
                     /locus_tag="ACHOSM_RS18880"
                     /old_locus_tag="pgaptmp_003776"
     CDS             complement(584..763)
                     /gene="yoaH"
                     /locus_tag="ACHOSM_RS18880"
                     /old_locus_tag="pgaptmp_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HGU4560190.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            837..2198
                     /gene="pabB"
                     /locus_tag="ACHOSM_RS18885"
                     /old_locus_tag="pgaptmp_003777"
     CDS             837..2198
                     /gene="pabB"
                     /locus_tag="ACHOSM_RS18885"
                     /old_locus_tag="pgaptmp_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HGU4560191.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRLVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2202..2780
                     /gene="nudL"
                     /locus_tag="ACHOSM_RS18890"
                     /old_locus_tag="pgaptmp_003778"
     CDS             2202..2780
                     /gene="nudL"
                     /locus_tag="ACHOSM_RS18890"
                     /old_locus_tag="pgaptmp_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HGU4560192.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2964..4328
                     /gene="sdaA"
                     /locus_tag="ACHOSM_RS18895"
                     /old_locus_tag="pgaptmp_003779"
     CDS             2964..4328
                     /gene="sdaA"
                     /locus_tag="ACHOSM_RS18895"
                     /old_locus_tag="pgaptmp_003779"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU4560193.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4459..6057
                     /gene="pdeD"
                     /locus_tag="ACHOSM_RS18900"
                     /old_locus_tag="pgaptmp_003780"
     CDS             4459..6057
                     /gene="pdeD"
                     /locus_tag="ACHOSM_RS18900"
                     /old_locus_tag="pgaptmp_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4560194.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLTVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHDVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6061..7617)
                     /gene="yoaE"
                     /locus_tag="ACHOSM_RS18905"
                     /old_locus_tag="pgaptmp_003781"
     CDS             complement(6061..7617)
                     /gene="yoaE"
                     /locus_tag="ACHOSM_RS18905"
                     /old_locus_tag="pgaptmp_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HGU4560195.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIMEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7605..7763)
                     /gene="yoaL"
                     /locus_tag="ACHOSM_RS18910"
                     /old_locus_tag="pgaptmp_003782"
     CDS             complement(7605..7763)
                     /gene="yoaL"
                     /locus_tag="ACHOSM_RS18910"
                     /old_locus_tag="pgaptmp_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HGU4560196.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8080..9051
                     /gene="manX"
                     /locus_tag="ACHOSM_RS18915"
                     /old_locus_tag="pgaptmp_003783"
     CDS             8080..9051
                     /gene="manX"
                     /locus_tag="ACHOSM_RS18915"
                     /old_locus_tag="pgaptmp_003783"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HGU4560197.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9114..9914
                     /gene="manY"
                     /locus_tag="ACHOSM_RS18920"
                     /old_locus_tag="pgaptmp_003784"
     CDS             9114..9914
                     /gene="manY"
                     /locus_tag="ACHOSM_RS18920"
                     /old_locus_tag="pgaptmp_003784"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HGU4560198.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9927..10778
                     /gene="manZ"
                     /locus_tag="ACHOSM_RS18925"
                     /old_locus_tag="pgaptmp_003785"
     CDS             9927..10778
                     /gene="manZ"
                     /locus_tag="ACHOSM_RS18925"
                     /old_locus_tag="pgaptmp_003785"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HGU4560199.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10833..11291
                     /gene="yobD"
                     /locus_tag="ACHOSM_RS18930"
                     /old_locus_tag="pgaptmp_003786"
     CDS             10833..11291
                     /gene="yobD"
                     /locus_tag="ACHOSM_RS18930"
                     /old_locus_tag="pgaptmp_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HGU4560200.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVLTQ"
     gene            11720..12286
                     /gene="mntP"
                     /locus_tag="ACHOSM_RS18935"
                     /old_locus_tag="pgaptmp_003787"
     CDS             11720..12286
                     /gene="mntP"
                     /locus_tag="ACHOSM_RS18935"
                     /old_locus_tag="pgaptmp_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HGU4560201.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKSKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(12283..13092)
                     /gene="rlmA"
                     /locus_tag="ACHOSM_RS18940"
                     /old_locus_tag="pgaptmp_003788"
     CDS             complement(12283..13092)
                     /gene="rlmA"
                     /locus_tag="ACHOSM_RS18940"
                     /old_locus_tag="pgaptmp_003788"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HGU4560202.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(13258..13467)
                     /gene="cspE"
                     /locus_tag="ACHOSM_RS18945"
                     /old_locus_tag="pgaptmp_003789"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(13258..13467)
                     /gene="cspE"
                     /locus_tag="ACHOSM_RS18945"
                     /old_locus_tag="pgaptmp_003789"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU4560203.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(13480..13623)
                     /gene="yobF"
                     /locus_tag="ACHOSM_RS18950"
                     /old_locus_tag="pgaptmp_003790"
     CDS             complement(13480..13623)
                     /gene="yobF"
                     /locus_tag="ACHOSM_RS18950"
                     /old_locus_tag="pgaptmp_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HGU4560204.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT         3526 a         3505 c         3477 g         3593 t
ORIGIN      
        1 tttctccatc ttactggata acacaccatg agctgctacc tttaaaacac ctgaatgtca
       61 acaggttaac tcgtgccata tcgttataat ccctttgata ttagatgcaa attaaggtca
      121 tatatagcct tatcgataac atggttaatt ttaaagataa gaatatgcct gctgtaatag
      181 ataaagccct ggatttcatt ggtgccatgg atgtatcagc gccaacacca agttcgatga
      241 atgaaagcac ggcgaaggga atctttaaat atttaaaaga actgggagta cccgcgagtg
      301 ccgctgatat tacggcgcga gctgaccagg aaggctggaa ccctgggttc acggaaaaaa
      361 tggttggatg ggcaaaaaaa atggagacag gtgaacgttc tgtgattaaa aatcctgaat
      421 acttttcaac atatatgcag gaagaactaa aagcactggt ctgagttaaa tttatatcag
      481 cataaatggg tcaggacgct tttaatcaca tataaaaagc gtcctttttc tcacaatcaa
      541 atgatcatcg gcttctataa cgaaatcgat acggattagt gtgttactcg tcttcatcct
      601 caaaacgcgc cacgattcgc tcgccggagt ggttggcacg cagctcttct gccaccagcg
      661 cgattgcctg gccgctgctc atcccttgtg ccatcagttc ctggatccgc tcgacagctt
      721 tttgctgctg ttcatgggtg agtgaaggta aacctgcaaa cattgttaac tcctgctaaa
      781 ttgttggcgc taattatttc atgctacccg gcacatagcc agtagagtca ggactgatga
      841 agacgttatc tcccgctgtg attactttac cctggcgtca ggacgccgct gaattttatt
      901 tctcccgctt aagccacctg ccgtgggcga tgcttttaca ctccggctat gccgatcatc
      961 cgtatagccg ctttgatatt gtggtcgccg atccgatttg cactttaacc actttaggta
     1021 aagaaaccgt tgttagtgaa agcgaaaaac gcacaacgac cactgatgac ccgctacagg
     1081 tgctccagca ggtgctggat cgcgcagaca ttcgcccaac gcataacgaa gatttgccat
     1141 ttcagggcgg cgcactgggg ttgtttggct acgatctggg ccgccgtttt gagtcactgc
     1201 cagaaattgc ggaacaagat atcgttctgc cggatatggc agtgggtatc tacgattggg
     1261 cgctcattgt cgaccaccag cgtcatacag tttctttgct gagtcataat gatgtcaatg
     1321 cccgtcgggc ctggctggaa agccagcaat tctcgccgca ggaagatttc acgctcactt
     1381 ccgactggca atccaatatg acccgcgagc agtacggcga aaaatttcgc ctggtacagg
     1441 aatatctgca cagcggtgat tgctatcagg tgaatctcgc ccagcgtttt catgcgacct
     1501 attctggcga tgaatggcag gcattccttc agcttaatca ggccaaccgc gcgccattta
     1561 gcgctttttt acgtcttgaa cagggtgcaa ttttaagcct ttcgccagag cggtttattc
     1621 tttgtgataa tagtgaaatc cagacccgcc cgattaaagg cacgctacca cgcctgcccg
     1681 atcctcagga agatagcaaa caagcagaaa aactggcgaa ctcagcgaaa gatcgtgccg
     1741 aaaatctgat gattgtcgat ttaatgcgta atgatatcgg tcgtgttgcc gtagccggtt
     1801 cggtaaaagt accagagctc ttcgtggtgg aacccttccc tgcagtgcat catctggtca
     1861 gcactataac ggcgcgacta ccagaacagt tacacgccag cgatctgctg cgcgcagctt
     1921 ttcctggtgg ctcaataacc ggggctccga aagtacgggc tatggaaatt atcgacgaac
     1981 tggaaccgca gcgacgtaat gcctggtgcg gcagcattgg ctatttgagc ttttgcggca
     2041 acatggatac cagcattact atccgcacgc tgactgccat taacggacaa atatactgct
     2101 ctgcgggcgg tggaattgtc gccgatagcc aggaagaagc ggaatatcag gaaacttttg
     2161 ataaagttaa taagatatta cgccaactgg agaagtaaga cgtggaatac cgtagcctga
     2221 cgcttgatga ttttttatct cgctttcaac ttttgcgccc gcaaattaac cgggaaaccc
     2281 taaatcatcg tcaggctgcc gtgttaatcc ccatcgtccg tcgaccgcaa ccggggttgt
     2341 tgctgactca gcgttcaatt catctgcgta aacacgctgg acaagtggca ttccctggag
     2401 gtgcagtcga tgacacggac gcatcagtta tcgccgccgc gctgcgcgaa gctgaagaag
     2461 aggtcgctat accgccttcc gccgttgaag ttatcggcgt gctgccgccc gtcgatagcg
     2521 taactggcta tcaggtaacc ccagtggtcg gcattatccc gcccgatctg ccgtatcgcg
     2581 ccagtgaaga tgaagtctcg gcggtgtttg aaatgccgct cgcccaggca ttacatctgg
     2641 gtcgttatca ccctttagat atctaccgcc gtggcgattc acatcgggta tggctgtcct
     2701 ggtacgaaca gtattttgta tggggaatga ccgcaggcat aattcgtgag ctggcgctgc
     2761 aaattggtgt gaaaccctga ctatacttat ctttacatct acaaaacact acttgagaca
     2821 atcatcgcaa tattagttaa atcgcggttt ttgattagtt taattcatgt gaatagttaa
     2881 gccagtcgcc gcgttccctc ttacactatg cgctgttatt agttcgttac tggaagtcca
     2941 gtcaccttgt caggagtatt atcgtgatta gtctattcga catgtttaag gtggggattg
     3001 gtccctcatc ttcccatacc gtagggccta tgaaggcggg taaacagttc gtcgatgatc
     3061 tggtcgaaaa aggcttactg gatagcgtta ctcgcgttgc cgtggacgtt tatggttcac
     3121 tgtcgctgac gggtaaaggc caccacaccg atatcgccat tattatgggt cttgcaggta
     3181 acgaacctgc caccgtggat atcgacagta ttcccggttt tattcgcgac gtagaagagc
     3241 gagaacgtct gctgctggca cagggacggc atgaagtgga tttcccgcgc gacaacggga
     3301 tgcgttttca taacggcaac ctgccgctgc atgaaaacgg tatgcaaatc cacgcctata
     3361 acggcgatga agtcgtctac agcaaaactt attattccat cggcggcggt tttatcgtcg
     3421 atgaagaaca ctttggtcag gatgctgcca acgaagtgag cgtgccgtat ccgttcaaat
     3481 ctgccaccga actgctcgcg tactgtaatg aaaccggcta ttcgctgtct ggtctcgcta
     3541 tgcagaacga actggcgctg cacagcaaga aagagatcga cgagtatttc gcgcatgtct
     3601 ggcaaaccat gcaggcatgt atcgatcgcg ggatgaacac cgaaggtgta ctgccaggcc
     3661 cgctgcgcgt gccacgtcgt gcgtctgccc tgcgccggat gttagtttcc agcgataaac
     3721 tgtctaacga tccgatgaat gtcattgact gggtaaacat gtttgcgctg gcagttaacg
     3781 aagaaaacgc cgccggtggt cgtgtggtaa ctgcgccaac caacggtgcc tgcggtatcg
     3841 ttccggcagt gctggcttac tatgaccact ttattgaatc ggtcagcccg gacatctata
     3901 cccgttactt tatggcagcg ggcgcgattg gtgcattgta taaaatgaac gcctctattt
     3961 ccggtgcgga agttggttgc cagggcgaag tgggtgttgc ctgttcaatg gctgctgcgg
     4021 gtcttgcaga actgctgggc ggtagcccgg aacaggtttg cgtggcggcg gaaattggca
     4081 tggaacacaa ccttggttta acctgcgacc cggttgcagg gcaggttcag gtgccgtgca
     4141 ttgagcgtaa tgccattgcc tctgtgaagg cgattaacgc cgcacggatg gctctgcgcc
     4201 gcaccagtgc accgcgcgtc tcgctggata aggtcatcga aacgatgtac gaaaccggta
     4261 aggacatgaa cgccaaatac cgcgaaacct cacgcggtgg tctggcaatc aaagtccagt
     4321 gtgactaata cttcttactc gcccatctgc aacggatggg cgaatttata cccgctttct
     4381 cgtctgctgt aatattcccc actacacttc cactgttgcg tcaggcgttt gtcgccatac
     4441 gcttacaggg tggcccgcat gcaaaaagca caacggatca ttaaaaccta tcgtcgtaat
     4501 cgaatgattg tttgtacgat ttgcgcactc gttacgctcg cttcgaccct gagcgtgcga
     4561 tttatttcac agcgtaactt aaatcaacaa cgggtagtac aattcgccaa tcacgctgta
     4621 gaggaattag ataaagtact gcttccccta caggcaggta gcgaagtctt gcttccgctg
     4681 attggtctgc cctgctctgt cgcccatttg ccattacgta aacaggcggc aaaactccaa
     4741 actgtgcgat ccattggcct ggtgcaagac ggcacactct attgctccag catttttggt
     4801 tatcgcaatg tgcccgtcgt ggacattctg gctgaacttc ctgcaccgca accactttta
     4861 cgcctgacga tcgaccgtgc cctgattaaa ggcagtccgg ttttgattca atggacgcct
     4921 gcagcgggca gtagcaatgc tggggtcatg gagatgatta acatcgactt actgacggca
     4981 atgctccttg agccacaact gccgcaaatc agtagcgcca gcctgacggt ggacaaacgg
     5041 catttgctct atggtaatgg gctggtagat tcccttccgc aacctgaaga caatgaaaac
     5101 tatcaggttt cttcgcaacg ctttcctttt accattaacg ttaatggtcc gggggctacg
     5161 gcgctggcat ggcactatct tccaacacaa ttaccgctga cggtgctgct aagtttactg
     5221 gtgggctaca tcgcctggct ggcgaccgct taccggatga gcttttcccg cgaaatcaat
     5281 ctgggcctgg cgcaacatga gttcgaattg ttctgtcagc ctttgcttaa tgcgcgcagc
     5341 cagcaatgta ttggtgtaga gattttgctg cgctggaaca atccgcgtca gggctggatt
     5401 tcaccggatg tgtttattcc tatcgcggaa gaacatcatt taattgtgcc actgacccgc
     5461 tatgtgatgg cagaaaccat tcgtcagcgc catgttttcc cgatgagtag tcagtttcat
     5521 gttggcatta acgtcgcacc cagccatttt cgccgtggtg tgctgataaa agatctcaat
     5581 cagtactggt ttagcgctca cccgattcag caactgatcc tcgaaatcac cgaacgcgat
     5641 gccttactgg atgttgatta tcggattgcc cgcgagctac atcgtaaaaa cgtcaaactg
     5701 gcgattgatg acttcggcac cggcaacagt tcattttcct ggcttgaaac attacgtcct
     5761 gacgtgctga aaattgataa gtcatttacc gcagctatag gttctgacgc ggttaactcg
     5821 acggtgaccg atatcatcat cgcgctgggg caaagactga atattgaact ggtggcggag
     5881 ggcgtggaaa cgcaagaaca ggcgaagtat ttgcgccgtc atgacgtgca tattttgcaa
     5941 gggtatttgt acgcacagcc gatgccgcta cgtgattttc ccaaatggct ggcgggcagc
     6001 caaccgccgc ccgcccggca taatggacat atcacgccca ttatgccgtt acgttaacgc
     6061 ttactcatct tcatcgtgcg ccggttgctc tttaacaatg cgaaccagat caacacgata
     6121 atcattggct tccatgatgg tgatatgcag tggcccaaca tcaatcacat cgcccacacg
     6181 gggaatgtga ccatttgccg agatcaccag gcccgcgacc gtcgcgatat catcgtcatc
     6241 ggcaaggtgc tcaacatcaa gcgcctgctg caaggcatgc aaatctgtac cgccttttac
     6301 cagccagccg tcaccatcgg taatgatttc cggcgtttcg tcagcgtccg ggaattcacc
     6361 cgcaatggct tccagcacat ccagcggcgt gaccaagcct tgtaccacac caaactcgtt
     6421 ggtcacgata acaaagctcc cgcgagcgcg acgcagcacg cccagcaggt tgatcggatc
     6481 gagggtttcc gggacgataa tcgccggaga cgccgaagca atcgccgcca catcaacgcc
     6541 ctcttccagc gccaccagca gttctttagc acgtacgatt ccgatgattt catccagttc
     6601 accgcgacat accgggaaca ggctgtgcgg tgaggagagc agctgctcgc ggatttcatc
     6661 gaccccgaga ttagcgtcaa cccagcttat ttcaccgcgc ggcgtcatga tcccgcgcag
     6721 agaacgcgac gccagcgtca gtacgccgtt aatcatgtaa cgttcttctt cggcaaatgc
     6781 accttccggg atcggcatcg gcatcgggtt atcggcatcg tgctgaacat tggcctgacg
     6841 tttcccgccc atcaaacgca ggatggcatc ggcagtacgc gctcgcagcg gcaaagtcga
     6901 ctggtgacga ataaagttgc gacgcgcaat ctggttaaac acttcgatga tgatcgagaa
     6961 gccaatcgcg gcatataggt aacctttcgg aatgtggaaa ccgaaacctt ctgccaccag
     7021 actcagacca atcattaaca ggaagctcag acagagcacc accaccgtgg ggtgctggtt
     7081 aacgaatcgc gtcagcggtt tcgatgccag caacataacc gccatcgcaa tcactaccgc
     7141 cgccatcatc accggcagat ggttaaccat ccctactgca gtaattaccg catccaacga
     7201 gaagacggcg tcaaggatga caatctgtgt gacgaccacc cagaaactgg cgtagccttt
     7261 accgtggccg gaatcatgat cgcggttttc cagccgttca tgcagttcgg ttgttgcttt
     7321 gaacagcaag aatatccccc cgaacaacat aatcaggtcg cgtccggaga aggagaaatc
     7381 catgacggta aatagcggtt tggtcagcgt gaccatccat gaaatcagcg acagcagccc
     7441 cagacgcata atcagcgcca gtgataaccc cagcaaacgc gctttatcgc gttgttttgg
     7501 cggcagtttg tcagcaagaa tggcgatgaa gaccaggtta tcgataccca gcacaatttc
     7561 gagaacaaca agcgtgagta gccccgccca aattgagggg tccattaaga attccatgac
     7621 aagctcctgc ttaaggaata gctattcgac gccagaaata atgcaggcgt aacgacaaaa
     7681 tgcaaacgaa aggtgcggca tagagtgcca gaaaggcagg cgttaaaagg cctgatgctg
     7741 aaatgacgtc ggtgacgatc catactgcgg gctactgccc tatactccat ggttgttaaa
     7801 cgggagttaa acatatcaga gacgcctctg atttggcaaa gatttacctt cctttgcaaa
     7861 cgaatgtgac aaggatattt tacctttcga aatttctgct aatcgaaagt taaattacgg
     7921 atcttcatca cataaaataa ttttttcgat atctaaaata aatcgcgaaa cgcaggggtt
     7981 tttggttgta gcccttatct gaatcgattc gattgtggac gacgattcaa aaatacatct
     8041 ggcacgttga ggtgttaacg ataataaagg aggtagcaag tgaccattgc tattgttata
     8101 ggcacacatg gttgggctgc agagcagttg cttaaaacgg cagaaatgct gttaggcgag
     8161 caggaaaacg tcggctggat cgatttcgtt ccaggtgaaa atgccgaaac gctgattgaa
     8221 aagtacaacg ctcagttggc aaaactcgac accactaaag gcgtgctgtt tctcgttgat
     8281 acatggggag gcagcccgtt caatgctgcc agccgcattg tcgtcgacaa agagcattat
     8341 gaagtcattg caggcgttaa cattccaatg ctcgtggaaa cgttaatggc tcgtgatgat
     8401 gacccaagct ttgatgaact ggttgcgctg gcagtagaaa caggccgtga aggcgtgaaa
     8461 gcactgaaag ccaaaccggt tgaaaaagcc gcgccagcac ccgctgccgc agcaccaaaa
     8521 gcggctccaa ctccggcaaa accaatgggg ccaaacgact acatggttat tggccttgcg
     8581 cgtatcgacg accgtctgat tcacggtcag gtcgccaccc gctggaccaa agaaaccaat
     8641 gtctcccgta ttattgttgt tagtgatgaa gtggctgcgg ataccgttcg taagacactg
     8701 ctcacccagg ttgcacctcc gggcgtaaca gcacacgtag ttgatgttgc caaaatgatt
     8761 cgcgtctaca acaacccgaa atatgctggc gaacgcgtaa tgctgttatt taccaaccca
     8821 acagatgtag agcgtctcgt tgaaggcggc gtgaaaatca cctctgttaa cgtcggtggt
     8881 atggcattcc gtcagggtaa aacccaggtg aataacgcgg tttcggttga tgaaaaagat
     8941 atcgaggcgt tcaagaaact gaatgcgcgc ggtattgagc tggaagtccg taaggtttcc
     9001 accgatccga aactgaaaat gatggatctg atcagcaaaa tcgataagta acgtattgtg
     9061 ttgattatca ctcagttttc acacttaagt cttacgtaaa caggagaagt acaatggaga
     9121 ttaccactct tcaaattgtg ctggtattta tcgtagcctg tatcgcaggt atgggatcaa
     9181 tcctcgatga atttcagttt caccgtccgc taatcgcgtg taccctggtg ggtatcgttc
     9241 ttggggatat gaaaaccggt attattatcg gtggtacgct ggaaatgatc gcgctgggct
     9301 ggatgaacat cggtgctgca gttgcgcctg acgccgctct ggcttctatc atttctacca
     9361 ttctggttat cgcaggtcat cagagcattg gtgcaggtat cgcactggca atccctctgg
     9421 ccgctgcggg ccaggtactg accatcatcg ttcgtactat taccgttgct ttccagcacg
     9481 ctgcggataa ggctgctgat aacggcaacc tgacagcgat ttcctggatc cacgtttctt
     9541 ctctgttcct gcaagcaatg cgtgtggcta ttccggccgt catcgttgcg ctgtctgttg
     9601 gtaccagcga agtacagaac atgctgaatg cgattccgga agtggtgacc aatggtctga
     9661 atatcgccgg tggcatgatc gtggtggttg gttatgcgat ggttatcaac atgatgcgtg
     9721 ctggctacct gatgccgttc ttctacctcg gcttcgtaac cgcagcattc accaacttta
     9781 acctggttgc tctgggtgtg atcggtactg ttatggcagt gctctacatc caacttagcc
     9841 cgaaatacaa ccgcgtagcc ggtgcgcctg ctcaggctgc tggtaacaac gatctcgata
     9901 acgaactgga ctaacaggtg agcgaaatgg ttgatacaac tcaaactacc accgagaaaa
     9961 aactcactca aagtgatatt cgtggcgtct tcctgcgttc taacctcttc cagggttcat
    10021 ggaacttcga acgtatgcag gcactgggtt tctgcttctc tatggtaccg gcaattcgtc
    10081 gcctctaccc tgagaacaac gaagctcgta aacaagctat tcgccgtcac ctggagttct
    10141 ttaacaccca gccgttcgtg gctgcgccga ttctcggcgt aaccctggcg ctggaagaac
    10201 agcgtgctaa tggcgcagag atcgacgacg gtgctatcaa cggtatcaaa gtcggtttga
    10261 tggggccgct ggctggtgta ggcgacccga tcttctgggg aaccgtacgt ccggtatttg
    10321 cagcactggg tgccggtatc gcgatgagcg gcagcctgtt aggtccgctg ctgttcttca
    10381 tcctgtttaa cctggtgcgt ctggcaaccc gttactacgg cgtagcgtat ggttactcca
    10441 aaggtatcga tatcgttaaa gatatgggtg gtggcttcct gcaaaaactg acggaagggg
    10501 cgtctatcct cggcctgttt gtcatggggg cattggttaa caagtggaca catgtcaaca
    10561 tcccgctggt tgtctctcgc attactgacc agacgggcaa agaacacgtt actactgtcc
    10621 agactattct ggaccagtta atgccaggcc tggtaccact gctgctgacc tttgcttgta
    10681 tgtggctact gcgcaaaaaa gttaacccgc tgtggatcat cgttggcttc ttcgtcatcg
    10741 gtatcgctgg ttacgcttgc ggcctgctgg gactgtaaga ctgttgtaca ctaccggggc
    10801 cttttggccc cgttttttta tctggaggat taatgacaat cacggacctg gtactgattc
    10861 ttttcatcgc cgcactcctg gccttcgcga tctacgatca gttcatcatg ccccgccgta
    10921 acggccccac cctgctggca attcctttgc tccggcgtgg tcgcatcgat agcgttatct
    10981 tcgtcggatt gattgtcatt cttatctata acaacgtcac gaatcatggt gcgttaataa
    11041 cgacatggtt attaagcgca ctggctctga tgggttttta tatattctgg atccgcgttc
    11101 cgaagatcat ctttaaacaa aaaggttttt tcttcgccaa tgtctggatt gaatatagcc
    11161 gaatcaaagc gatgaacttg tcggaagatg gcgtgttggt gatgcaatta gaacagcgtc
    11221 ggctgttaat ccgcgttcga aatatcgacg atctggaaaa aatttataag cttctcgttt
    11281 taactcaata agttatgaat ttagccaaag ctatgtttag tgtattttta ataatcagac
    11341 atagcttagg ctatattacc tcttccctta tttgttattt attttaacgt ttcattgata
    11401 tataaatcta aatgaaaatc gttatcaata aagcaatgaa ataatatatt ccaacagttg
    11461 ttttatattc tcaaaatatg ttaaggttgc gccctcattt ggggagtagc cgatttccag
    11521 attccggaaa tgtacgtgtc aacatactcg ttgcaaaacg tggcacgtac ggactgaata
    11581 ctttcagtca ggcgagacca tatgcatatc aatcgctatg cctgcatgaa cgcgaaaacc
    11641 atcacggctt gttgttcatg tatgcatgtt tgctgggggc gatgatgtgt tttatggata
    11701 ccccggtcag gacattgtca tgaatatcac tgctactgtt cttcttgcgt ttggtatgtc
    11761 gatggatgca tttgctgcat caatcggtaa aggtgccacc ctccataaat cgaaattttc
    11821 tgaagcattg cgaaccggcc ttatttttgg tgccgtcgaa accctgacgc cgctgatcgg
    11881 ctggggaatg ggcatgttag ccagccggtt tgtccttgaa tggaaccact ggattgcgtt
    11941 tgtgctgctg atattcctcg gcgggcgaat gattattgag ggttttcgtg gcgcagatga
    12001 tgaagatgaa gagccgcgcc gtcgacacgg tttctggcta ctggtaacca ccgcgattgc
    12061 caccagcttg gatgccatgg ctgtgggtgt tggtcttgct ttcctgcagg tcaacattat
    12121 cgcgaccgca ttggccattg gttgtgcaac cttgattatg tcaacattag ggatgatggt
    12181 tggtcgcttt atcggctcaa ttattgggaa aaaagcggaa attctcggcg ggctggtact
    12241 gatcggcatc ggcgtccaga tcctctggac gcacttccac ggttaataag aacgctgcca
    12301 gaggtgaata ttaaagtccg tctggcagtc gaacacttct tttgctgcca gtgtttgcca
    12361 gacttctggc ttcgcacgcc aggcaaacgg cgtcatctgc aataatgcga cggcttcatc
    12421 accgcgaaga cgcatcggat aacacaactc cgcactctgc tgtaatgtaa aaccttccag
    12481 ttgttctgca tgaggtgcat gaagatgtac ttcattgtaa atcagcccct tcagctccat
    12541 caaatgtcgc ggtcccggcg tggcagtaat gacccagccg ccgggcttca ctactcgtgc
    12601 taattcttct gctttacacg gcgcgtaaat acgtattatg gcgtccatac tggtatcgga
    12661 aaacggcaaa cggtggctgg aagcgacaca aaaagtgacc tgcggatagc gtttcgccgc
    12721 cgcttttatc gctaccttcg aaacatccag accaaacgtg gtgatttcgg gcaacgcatc
    12781 ggcaaatgcg tgtgtgtaat acccttcacc acagccaata tccagcaccg ccgtggcctt
    12841 atcatcaagc cgttccctca gttgggcgac aattgcatca cgcagcggct gataatgtcc
    12901 ggcatctaag aatgcgcggc gtgcttgcat catttccgcg ctgtcgcccg gatcacgaga
    12961 ccgtttatgc tgaacgggca gcagattgac atacccttct ttcgccatat caaactgatg
    13021 tcgctgggga cagatatagc tgtttttttc acgcgaaaga ggctgatggc aaagtggaca
    13081 ggaaaaagac atgacaactc cggcatgata ctaaaggccg caagtgtaac gcgaatcacg
    13141 cggcgggcaa atatccgaac gccaggagcg aagataaagc atagtaaaaa gcctcgcatt
    13201 cgacgaggct ttatagcgag attgaagcgt attcacactt cagatcagtg gatttgatca
    13261 gatagctgtt acgttaacag ctgccggacc tttctggccg tcctgaattt cgaactcaac
    13321 gttctgacct tcagccagag ttttgaagcc attaccctgg atagcggaga agtgtacgaa
    13381 cacgtctttg ctgccatcag ccggagtaat gaagccaaaa cctttagact cgttgaacca
    13441 cttaacctga cctttaatct ttgccatttg aaaaattcct tagattgttt tcttcgcccg
    13501 caggcataac atagataaaa ctgacacatt actgcatgag gcaccaatat aaggctcggc
    13561 agagaagcgg tattcaacgt caacgtgttt actcaggact tctttactga aaatgccaca
    13621 cataaacaga actgtacctc gtttaacccg aaatctgtta tcacatacaa cgttaattat
    13681 ggcaagccat ttttaaacat gtctcgatca gacgcacaaa tctcgatacg tcttccactt
    13741 tttttgcaca ctaatgcaac ggaatacgcg ccgatatatc attgtgctta accttgccag
    13801 ttcaggcaga tacttaaact ggcgtatttt ctaacatagt tcaataagtt caattgttcc
    13861 aggccagtgg gagaagttat tacatagtgc gtgcaatatc acattttttg ctatgcaatg
    13921 aataaaaagt tatatcactt tttctcataa aacagtcagt taacggctat caattaccct
    13981 aaagagaagt caatccccaa agggattgaa aatttaaaat aagaaaaatt gatgaatgag
    14041 caaaaaatca agagagaaac gtttcgctaa taaataatac cacgcttaat cacaaatccg
    14101 a
//
LOCUS       DAZKHC020000104        14088 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZKHC020000104 DAZKHC020000000
VERSION     DAZKHC020000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.104"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..139
                     /locus_tag="ACHOSM_RS18955"
                     /old_locus_tag="pgaptmp_003791"
     CDS             <1..139
                     /locus_tag="ACHOSM_RS18955"
                     /old_locus_tag="pgaptmp_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGU4560205.1"
                     /translation="SGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGL
                     S"
     gene            410..2647
                     /gene="nrfB"
                     /locus_tag="ACHOSM_RS18960"
                     /old_locus_tag="pgaptmp_003792"
     CDS             410..2647
                     /gene="nrfB"
                     /locus_tag="ACHOSM_RS18960"
                     /old_locus_tag="pgaptmp_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HGU4560206.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2634..5606
                     /gene="nfrA"
                     /locus_tag="ACHOSM_RS18965"
                     /old_locus_tag="pgaptmp_003793"
     CDS             2634..5606
                     /gene="nfrA"
                     /locus_tag="ACHOSM_RS18965"
                     /old_locus_tag="pgaptmp_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HGU4560207.1"
                     /translation="MKENNLNRVIGWSGLLLTFLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDARARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELFAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5607..6497
                     /gene="ybcH"
                     /locus_tag="ACHOSM_RS18970"
                     /old_locus_tag="pgaptmp_003794"
     CDS             5607..6497
                     /gene="ybcH"
                     /locus_tag="ACHOSM_RS18970"
                     /old_locus_tag="pgaptmp_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HGU4560208.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6680..7441
                     /gene="envY"
                     /locus_tag="ACHOSM_RS18975"
                     /old_locus_tag="pgaptmp_003795"
     CDS             6680..7441
                     /gene="envY"
                     /locus_tag="ACHOSM_RS18975"
                     /old_locus_tag="pgaptmp_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HGU4560209.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7487..7563)
                     /locus_tag="ACHOSM_RS18980"
                     /old_locus_tag="pgaptmp_003796"
     tRNA            complement(7487..7563)
                     /locus_tag="ACHOSM_RS18980"
                     /old_locus_tag="pgaptmp_003796"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7527..7529),aa:Arg,seq:tct)
     gene            7806..8438
                     /gene="fimZ"
                     /locus_tag="ACHOSM_RS18985"
                     /old_locus_tag="pgaptmp_003797"
     CDS             7806..8438
                     /gene="fimZ"
                     /locus_tag="ACHOSM_RS18985"
                     /old_locus_tag="pgaptmp_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HGU4560210.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(8441..8956)
                     /gene="sfmF"
                     /locus_tag="ACHOSM_RS18990"
                     /old_locus_tag="pgaptmp_003798"
     CDS             complement(8441..8956)
                     /gene="sfmF"
                     /locus_tag="ACHOSM_RS18990"
                     /old_locus_tag="pgaptmp_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HGU4560211.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTIAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(8967..9974)
                     /gene="fimH"
                     /locus_tag="ACHOSM_RS18995"
                     /old_locus_tag="pgaptmp_003799"
     CDS             complement(8967..9974)
                     /gene="fimH"
                     /locus_tag="ACHOSM_RS18995"
                     /old_locus_tag="pgaptmp_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU4560212.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(9987..12596)
                     /gene="sfmD"
                     /locus_tag="ACHOSM_RS19000"
                     /old_locus_tag="pgaptmp_003800"
     CDS             complement(9987..12596)
                     /gene="sfmD"
                     /locus_tag="ACHOSM_RS19000"
                     /old_locus_tag="pgaptmp_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGU4560213.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQITL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQATYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(12627..13319)
                     /gene="fimC"
                     /locus_tag="ACHOSM_RS19005"
                     /old_locus_tag="pgaptmp_003801"
     CDS             complement(12627..13319)
                     /gene="fimC"
                     /locus_tag="ACHOSM_RS19005"
                     /old_locus_tag="pgaptmp_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU4560214.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVPPVREVNLN"
     gene            complement(13539..14081)
                     /gene="fimA"
                     /locus_tag="ACHOSM_RS19010"
                     /old_locus_tag="pgaptmp_003802"
     CDS             complement(13539..14081)
                     /gene="fimA"
                     /locus_tag="ACHOSM_RS19010"
                     /old_locus_tag="pgaptmp_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4560215.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT         3697 a         3520 c         3372 g         3499 t
ORIGIN      
        1 ttcagggata cggcacatcg ctattaatat tttaacgaat gataaggtat tcaaggcagg
       61 gttaagacgt aagatgcgaa aagcagccat ggacagaaac tatctcgcgt cagtccttgc
      121 ggggagcggg ctttcgtaat cttgccctgc ctgctcacct cctccaggca cttccactac
      181 atgatgacct gctcggtgcc accgctcctt ttcactatga cgttaacctc cccgtcccag
      241 gacaccatgc ttttgtctac attctacgca aacggcaact tttataacca gtgtaaaaat
      301 aacgtgccgc aatagtcata cctattaatg gtaaaaagct gtcacaattc ataaaaaacc
      361 ttaatatacg ccaccctaaa cataaccagc gttaatgtaa ggtttttgtg tggactggct
      421 tcttgatgtt tttgcaacct ggctctacgg cttaaaagta atcgcgataa cgttagcggt
      481 catcatgttc atcagcgggc tggacgattt ttttattgat gtcgtctact gggtacgccg
      541 cattaaacgc aagttgagtg tttatcgccg ctacccgcga atgagttacc gcgaactgta
      601 taaaccagat gaaaaaccgt tagcgattat ggttccggcg tggaatgaaa cgggcgtcat
      661 cggcaatatg gccgagctgg cggcgaccac gctcgactac gaaaactatc atatctttgt
      721 tggcacctac cccaacgacc ccgatactca acgtgatgtt gacgaagtgt gcgctcgctt
      781 tccgaacgtg cataaggtag tctgcgcgcg tcctggtccc accagcaaag ccgactgtct
      841 gaacaacgtg ctggacgcca tcacccagtt tgaacgtagc gccaatttcg cttttgctgg
      901 ttttattctg catgacgccg aagatgtgat ttcaccgatg gaattgcgtc tgttcaacta
      961 tctggtcgag cgtaaagatc tgattcagat cccagtgtat ccgtttgaac gcgaatggac
     1021 gcacttcacc agcatgactt acattgatga gttttcagaa ctgcatggca aagatgttcc
     1081 ggtgcgtgaa gccctcgccg gacaagtgcc cagcgcaggc gtcggcacct gtttcagccg
     1141 ccgcgccgtg accgctctgt tagctgacgg tgacggtatt gctttcgacg tgcagagtct
     1201 tactgaagat tacgacattg gcttccgcct gaaagaaaaa ggtatgacgg aaatttttgt
     1261 ccgttttccg gtggtggacg aagccaaaga acgcgagcag cgtaaatttt tacagcacgc
     1321 acggacgtca aacatgatct gcgtgcgcga atatttcccc gatacctttt cgactgcagt
     1381 tcgacaaaaa tctcgctgga tcatcggcat tgttttccag ggctttaaaa cccacaaatg
     1441 gacctccagc ctgacgctga actactttct ctggcgcgac cgcaaagggg caatcagtaa
     1501 ctttgtcagc ttcctcgcga tgctggtgat gctccagctt ttgctgttgc tggcgtatga
     1561 aagtttgtgg cccgatgcct ggcatttcct ttctattttt agcggcagcg catggttaat
     1621 gaccctgctg tggctaaact ttggcttgat ggttaaccgc atcgtgcagc gggtgatttt
     1681 cgtcactggc tactacggtc tgacgcaggg gctgctatct gtcctgcgtc ttttctgggg
     1741 caacctgatt aacttcatgg ccaactggcg cgcgctaaaa caggtacttc aacacggcga
     1801 tccacgtcgc gtcgcgtggg ataaaacaac gcatgacttc cccagcgtga ctggcgatac
     1861 ccgctcgttg cgcccgttag gccaaattct gctggaaaat caggtcatca ctgaagaaca
     1921 actcgataca gcactgcgta atcgcgtcga aggtctacgc ctgggcggtt caatgctgat
     1981 gcaggggctg attagcgccg agcagctggc acaggcgctg gcagagcaaa acggcgtggc
     2041 gtgggaatcc atcgatgcct ggcagatccc gtcctcgctg attgccgaaa tgccggcctc
     2101 cgtggcgctg cattatgcgg tactgccgct gcgtctggaa aatgatgagt taattgtcgg
     2161 cagtgaagat ggtattgacc cggtttcgct ggcggccctg acgcgtaaag tcggacgcaa
     2221 agtgcgttac gtcattgttc tgcggggaca aattgtcacg gggttacgtc actggtatgc
     2281 acgccgacgc ggtcacgatc cgcgggcaat gttgtacaat gcggttcagc atcagtggct
     2341 cacggaacag cagaccggtg aaatctggcg gcaatatgtg ccgcatcagt tcctgttcgc
     2401 cgaaatactg accacgctcg gtcatattaa tcgttcagca attaacgtgt tgttattgcg
     2461 ccatgaacgc agttctctgc cgctcggcaa gtttttggtc accgaaggcg ttatcagcca
     2521 ggaaacgttg gatcgcgtcc tgacaattca acgcgaatta caagtttcga tgcaatcact
     2581 attactcaaa gcaggtttaa acacagaaca ggttgcgcaa ctggagtccg aaaatgaagg
     2641 agaataacct taatcgcgtc atcggatggt ctggtttact gctgacgttt ttattgagta
     2701 ccagcgcact cgcagacaat atcggcacca gcgcagaaga gctggggctg agcgattatc
     2761 gccattttgt tatttatccc cgtctcgata aggcgctgaa ggcacagaaa aataacgacg
     2821 aagcaaccgc catccgcgaa tttgaatata tacaccagca ggtgccggat aatattccgc
     2881 tgactttata ccttgcggaa gcctatcgcc attttggtca tgatgcccgg gcgcggctgt
     2941 tgcttgagga tcaactgaaa cgtcacccag gagatgcccg acttgagcgc agtctggcgg
     3001 ctattccagt tgaagtgaaa agcgttacga cagttgaaga actgtttgcc cagcaaaaag
     3061 cgtgcgatgc tgcgccgacc ctgcgttgtc gcagtgaagt cgggcagaat gccctgcggc
     3121 tggcacagtt acctgtcgcc agagcgcaac tgaacgatgc gacgtttgct gcatcgccag
     3181 aaggaaaaac gctgcgaacc gatctgctgc aacgggcaat ctacctgaaa caatggtccc
     3241 aggcagatac gctatacaat gaagcacgcc agcagaacac attaagcgcg gcagaacgcc
     3301 gtcagtggtt tgacgtgctt cttgccgggc agctggacga tcggatcctg gcactgcaat
     3361 cacaggggat cttcaccgat cctcagtcat atattactta cgcgaccgcg ctggcttatc
     3421 gtggcgaaaa agcacgcctc cagcattatc tcaatgaaaa taagccgctg tttaccacgg
     3481 acgcacaaga gaaaagttgg ctctatctgt tatctaaata cagcgccaac cccgttcagg
     3541 cattggcgaa ttatacggtg cagtttgccg ataaccgcca gtatgttgtt ggcgcgacgc
     3601 taccggtgct gttaaaagaa ggtcagtacg acgcagcgca aaaactgctc gccaccctcc
     3661 ccgccaatga aatgcttgag gagcgttatg ctgtcagcgt ggcgacccgt aacaaggctg
     3721 aatctctgcg tctggcacga ttgctgtatc agcaagaacc ggcaaatctt acccgcctgg
     3781 atcaactgac ctggcaactg atgcagaacg agcagtcacg cgaagctgcc gatttattgc
     3841 tgcaacgcta tcctttccag ggcgatgcgc gtgtcagcca gactttaatg gcgcgactgg
     3901 cgtctctgct ggaaagtcat ccttacctgg caacgccggc gaaggtggcg attttatcga
     3961 aacccttacc gctggcggag caacgtcagt ggcaaagtca gttgccgggt attgcagata
     4021 attgcccggc aatagttcgc ttgctgggcg atatgtcgcc ttcctacgat gctgccgcct
     4081 ggaaccgtct ggcaaagtgt tatcgggaca cgctacccgg tgtggcgctg tatgcatggc
     4141 ttcaggccga acaacgacaa ccgaacgcct ggcaacatcg tgcggtagcc tatcaggcgt
     4201 atcaggttga ggactacgcc accgcactgg cggcctggca gaaaatcagt cttcacgaca
     4261 tgagcaatga ggatctgctt gctgctgcca ataccgccca ggcggcagga aatggtgcag
     4321 ctcgcgatcg ctggctacaa caggcagaac aacgtggact gggaaacaat gccctctact
     4381 ggtggctgca tgcgcaacgt tacattcctg gtcagccgga actcgcactg agcgatctca
     4441 cgcgctcaat caatattgcg ccttctgcca acgcttacgt tgcgcgggcg acaatttatc
     4501 gccaacgtca taatgtcccg gcggcggtga gtgatttgcg cgccgcgctg gaactggaac
     4561 cgaataatag caacatccag gcagcgcttg gttacgcctt gtgggatagc ggtgatatcg
     4621 cacagtcgcg ggaaatgctc gaacaggcgc ataaagggct tccggacgat ccggcactga
     4681 tccgacaact ggcctacgtg aaccagcgtc tggatgacat gcctgcgacg cagcactacg
     4741 cccggctggt gattgatgac attgataatc aggcgctgat aaccccactg accccagagc
     4801 aaaatcagca acgcttcaat ttccgccgtt tgcatgagga ggtcggtcgc cgctggacgt
     4861 tcagtttcga ttcttctatc ggcttgcgtt ccggggcaat gagcaccgct aacaataatg
     4921 tcggcggcgc agcgccaggg aaaagctatc gtagctacgg gcaactggaa gccgagtacc
     4981 gcatcggacg caatatgctg ctggaaggcg acctgctctc agtttatagc cgcgtctttg
     5041 ccgataccgg agaaaacggg gtgatgatgc cggtgaaaaa tccgatgtcc ggcaccggtc
     5101 tgcgctggaa gccgctgcgc gatcagatct ttttcctcgc cgtcgaacag cagttgccgc
     5161 tgaacgggca aaatggcgca tccgatacca tgctgcgcgc cagcgcctca ttctttaatg
     5221 gcggcaaata cagcgacgaa tggcacccga acggttcagg ctggtttgcc caaaacctgt
     5281 acctcgatgc ggcgcaatat atccgccagg atattcaggc gtggacggca gattatcgcg
     5341 tcagctggca tcagaaggta gctaacggac agactattga gccttacgct cacgttcagg
     5401 acaacggcta tcgtgataaa ggcactcagg gcgcgcagct tggcggagtc ggggtccgct
     5461 ggaatatctg gaccggcgag acgcactacg acgcctggcc gcacaaagtc agtctcggcg
     5521 tcgagtatca acataccttt aaggcgatta atcaacgtaa cggagagcgc aacaacgcgt
     5581 ttctcaccat tggagtgcac tggtaaatgc gtaagttcat tttcgtattg ctgacactgc
     5641 ttttggtcag ccctttttcc tttgcgatga aaggtattat ctggcaacca caaaaccgag
     5701 atagtcaggt taccgatacc cagtggcagg ggctgatgag tcagttacgt ttgcaaggct
     5761 tcgataccct ggttttgcaa tggacccgtt acggcgatgc atttacccag ccagaacagc
     5821 gcacgttatt gtttaagcgt gccgcagctg cgcaacaggc tggcctgaag ctgattgtcg
     5881 ggctgaacgc cgatccggaa ttttttatgc accagaaaca gtcgtccgca gcgctggaaa
     5941 gctatcttaa tcgcctgctg gctgccgatc tccagcaagc cagattatgg agcgccgcgc
     6001 ctggcataac gccggatggc tggtacatca gcgcggaaat tgacgacctg aactggcgca
     6061 gcgaagctgc ccgtcagcct ttgctaacat ggttaaacaa cgcgcagcgg ctgattagcg
     6121 atgtttcagc aaaaccggtt tatatcagta gttttttcgc cggaaacatg tcgcccgatg
     6181 gctatcgcca actgctggaa cacgttaaag caaccggcgt taatgtttgg gtacaggatg
     6241 gcagcggcgt ggataaactg accgctgaac agcgtgaacg ttatctacag gccagcgccg
     6301 attgccaaag ttccgcccca gcaagcggca ttgtttatga actttttgtc gccggcaaag
     6361 gcaaaacctt tacagcgaaa ccgaaaccgg acgcagaaat tgcctcgctg ttagcgaaac
     6421 gttcctcttg cggtaaagac actctctatt tctctctgcg ctatttgccc gtcgcgcacg
     6481 gcattctcga gtattaaatc tcctccaggt aagtcgggta cgacctggct tacctctttc
     6541 gctctcgtca gaattccatc aagattaatt gctaaaaagc gtgctaaaga aagaagatcg
     6601 ttatcggcga acgggccgat cactcatgca ggaagcatag atcctttgag cggagtttct
     6661 gatatctgag gagtgcgaaa tgcaattgag cagcagtgaa ccttgcgtgg tgatcctgac
     6721 cgaaaaagag gtagaggtaa gcgtcaataa ccatgctacg tttacccttc cgaaaaacta
     6781 cctggccgcc ttcgcgtgca acaataacgt cattgaactc tcaacgttaa atcacgtatt
     6841 aatcacccac atcaaccgta acatcatcaa cgattatctg ttgtttttaa ataagaactt
     6901 aacctgtgta aagccctggt cgcggctggc aaccccggtt attgcttgtc atagccgtac
     6961 accggaagtg ttccggctag ccgccaacca cagcaagcag caacccagca aaccctgcga
     7021 agcggagttg acgcgcgcat tgctttttac cgtattgtct aactttcttg agcaatcgcg
     7081 gtttattgcc ctactgatgt atatcttacg cagcagcgtc cgcgacagcg tctgccgcat
     7141 tattcaaagc gatattcagc actactggaa tctgcgaatt gtcgccagtt cgctatgttt
     7201 aagccccagc ctgctcaaaa agaaattaaa aaacgaaaat accagctata gccagattgt
     7261 cacagagtgt cgtatgcgtt acgccgtaca gatgttattg atggataaca aaaatatcac
     7321 tcaggtggcg caattatgtg gctatagcag cacgtcgtac tttatctctg tttttaaggc
     7381 gttttacggc ctgacaccgt tgaattatct cgccaaacag cgacaaaaag tgatgtggtg
     7441 aagggcaaag cggaaacgga taagacgggc ataaatgaag aagaaatggc gcgccctgca
     7501 ggattcgaac ctgcggccca cgacttagaa ggtcgttgct ctatccaact gagctaaggg
     7561 cgcgttgata ccgcaatgcg gtgtaatcgc gtgaattata cggtcaaccc ttgctgagtc
     7621 aatggctttt gatctggttg ctgaacaagt gaacgaccgc gtctgatttt ctgatttatt
     7681 tcgctatagc ggcaaacaaa cgcacaccgc tgcgcgtctg aatcaagaaa acccgtattt
     7741 tcatgtatca aagtacaatt taccgcccta acggaaaatt gtccgctcct atgagactgg
     7801 taactatgaa accaacgtcg gtgatcatta tggatactca tcctatcatc agaatgtcta
     7861 ttgaagttct gttgcaaaaa aacagtgaat tgcagattgt cctgaaaacg gatgattacc
     7921 gcataaccat cgattatctc cgaacccgtc ctgttgattt aatcattatg gatatagact
     7981 tgcccggaac agacggtttt accttcctga aaaggatcaa acaaatccag agcacagtga
     8041 aagtgttatt tttatcatcg aaatcagaat gcttttatgc tggcagagcg atacaagctg
     8101 gtgctaacgg ttttgtcagt aaatgcaatg atcagaatga tatttttcat gccgttcaga
     8161 tgatcctctc cggatacacg ttttttccca gcgaaacgct taactatata aaaagcaata
     8221 aatgtagtac gaatagttca acggtcactg tgctatctaa tcgtgaagtg accatattac
     8281 gttatctggt tagcggatta tctaataaag aaattgccga taagttatta cttagcaata
     8341 aaacagttag tgcgcataaa tctaatattt atggcaagct aggtttgcat tcaattgtag
     8401 agcttatcga ctacgccaaa ttatacgaat taatataata ttaattataa ttgatcataa
     8461 atatcgcatc cgctttcgcc acacctggcc gaacaccgct ggctaacgct cgataattgg
     8521 caaaaaagtt tagtgtgaca ttgccatttg catctacttc ctcagtcggg ctcgccgctc
     8581 ccagtgcgag ccgggagcga tcgctattac gtaattcgat ggcgatggtt tgcgccattg
     8641 cgggatcatc cagagccagc aggttggtat cggatgccgg cgttcccgta aataaaatcg
     8701 caactgaacc cggaggacat ccctccagcc gcaggctaaa agggacgagt gccgtggtat
     8761 cgccagcgtt cagtaattgt gtcgtaggcc atctgcctaa atctaccgtc ttatcaatat
     8821 ccgcagtgtt tacggtacag gagaaatcaa caacgttacc gtgcaaattg atattaatcg
     8881 ttcccaaagg gtcagctgcc catccactgg aaatccacag taacccgcag aaacagctaa
     8941 agagtgctct tctcattctc cgtacctcat tgataatcga cgcgtaaata ccccagcgcg
     9001 ctaaacggcc cttcggtcgg tttttgaccg gtaatactga taggccaggc gcgaagtgtg
     9061 acattggctg ccgcagctgc atccagacgg aaaggaataa cgctattgag atcgttaggc
     9121 gtgatcggcg tatcgttctg atcggcgaca ataaaaccta aatcctgatt gtccgacacc
     9181 atcgcctgac cagaaacggc actggcttcc agacgcattg ttaaataagc ctgcgcagca
     9241 acattcgtac atttgaccgc aatgctcttg gtttgcggca tgacaccagc aggtcgattc
     9301 cccggccctg ccgcactaaa taacgatgcg ccgatatcac caaaatcaaa ttcaacaatc
     9361 tgcccggcat ttaattcgca gttttgcggt acttcaaccc ggccaccaaa actaatggta
     9421 taaacaggtg taaccaacgg atcgtaaggc gttgatgtca catacacggt aaacatcacc
     9481 tgtctgggga tctccaccat gttaataaag ggacgaataa cctttaaacg aaatattaaa
     9541 cgagaatcca tcaccccgaa tggaaggttt tgcgaaacgt taggatctgt tcccatccga
     9601 atataatttt gcggggggaa ctgaatatcc ataacactat caaccagact catcgcacct
     9661 aatagataat catttaattg catatattta taattatcga tagtttcctg aacaataaag
     9721 ttggtgacat aactacggta tgtataattc accagcgtgc cgggtggaca aattgctgac
     9781 acacctaccc agccggattt ttccggtaaa acaacaatct gccctggctg gttattggtg
     9841 ctattaaaga tatttgagag atcatagtgt tcatcttctg ctacgccagt actatttgca
     9901 caaacagtag cccaggatat gtttgccaga cacaaacatg ccatcgccaa taataatctg
     9961 cagattatct tcattgataa tttcctttat gagccaggat gtgtgcaaac tgccgaaata
    10021 acagtgacgg cttgctgtaa gctttgcttc ggcaatacat agtgcgcaat gcagcgtgag
    10081 ttcgcgccgt ctccccactg aaccagtaat tcaccagaca atggcgcacc acttaaataa
    10141 acttgcccgt catcacccac catactggtt attccggtac tgttttcccg taccagtgat
    10201 ccaaacggca ccggttttcc gccctgggta acggtaatga gcgcccgcac gcctatgcgg
    10261 gtatcaaaat tggcacgaac caacgcgcct tgcgtcggca caacgctgct aatatttttt
    10321 tcaacatcga tggaattccc catcgtattg gtatcaagcg cgatacggtt ataccgataa
    10381 accgtggcat acggcatcac cgcatagccg cgccagtcgg ttaaaatgcc agtttgattt
    10441 tcaatgcgta caccgcctgc gccaggcgct ttaatcaaaa cattggtatc ccctaaaggc
    10501 tggctcagcg ttatgccatt ttcgtgcccg accacaccgc cagacagctg ccagttaacg
    10561 tcatgttgat cgcggtcgta gttatacccg acccccagcg taccgtaagt ggcctgccag
    10621 tttgccgtgg cgctgcccgt atacccatta ttcgaggtat caccctggct tacgtgataa
    10681 ctcaggttgt ggccttccag taaggtccca cctacacctg ccagccagct attttgcccg
    10741 ttgctgttac ggttggcgtt aaaggaggca taagcgcggt cgagcgcatt ttcccgggtg
    10801 tagcgacgtt tggtcagaac attgaaaggc actgaaacat taagtccgac aatacgttcg
    10861 ttatcgggga tccctacaga ttcattccac gaaaatgaga gtgaataact gatgccaacc
    10921 cagctgctgg tataccccac ctgataccag gtatctgaat ccgaagtatt ccagtatttt
    10981 tgatgggtac cagagatata aagcgagcca aagtcattaa gtgattgtga aatatttaac
    11041 tgcaaacggt ctttacggct aaagcgtaaa ttgtggtaat tcacgattat cggttcatcc
    11101 cgatgctcac cgtcgtaatc gtaatcatat tcgtacccct ccattcgacg atacgcaaca
    11161 tcatcaaggg tataaaaacc ttgtgtcgaa tagcggtaac ccattaactg aaaattggtg
    11221 ccgaaggtgt tcatcgattt cgcatagagg aagcgaatgg aatccccctc atgacgactg
    11281 tcgtcggcta actgactgcg cgcatgggtc acatccagcg acactgcccc ccagtttccg
    11341 agattgcgcc ccagtcctaa taaaaaggcg gtgtaattgg cagataattg cgtcccgccg
    11401 taggcagtaa attcctgtgg taaaccgccg agtgccgtgc cctgaaaaaa gaaaggtgat
    11461 gattgctgac tattaccgct acgaaaatcg cccgccgtca ggtcaaattt gaaacgccct
    11521 tcgcgttgta aaatcggcac tgttgaatac ggaattgtgt aattctgctg attgccatcg
    11581 cgctcgtcga tggtgacatc cagatcgcca tttgaagatg tcgggtgcaa atctgtaatt
    11641 tcaaaagcgc cgggggaaac atagctttga tagataataa aaccattttg ccgaatcgtt
    11701 agctgggccg ccgtacgggc aatcccacgt accgttgggg caaacccttg ctggctatca
    11761 ggatacatat tatcggaaga gtaaagccgt ataccacgaa atccaacacc atcgaaaata
    11821 tcacttcctg tattgccgtc tcccattacc agttcacttt tcagcggaat aatggcgcgc
    11881 tgtacccagg tgccaatatt attccactgt tctgaatgat atccatttcc gcgaaaatag
    11941 ttccaggaac cattgttgcg taaacgccac gggccaatat taatcccgct gagctcacta
    12001 aaaaaatagc tatcgttacc attacctctg ttaccggtga aattataatt caggagtaaa
    12061 gcaggaatac cttcatccca ctcttcaggc ggaatgtaac cgtgagcgct actcaacaac
    12121 gttatctgag gaatagtgat gttcaggcgc atcgctgcaa aatcaaagtt aattgtggca
    12181 tcaggtacag ctttcagtaa attgatgcat ttattgtttt gctgctgggc taactcggga
    12241 aatgcactgc tattaaggcc aattcgttca aggagtacct ggttaaaaca gggcattaac
    12301 ccacctgatt tatcacctgt attttctgtt gtcgattcaa atacgatatc ctgtgaacca
    12361 atgaactcat cattacgcca gagatccacc cgatacacgc ccgcaggttg atgattacct
    12421 ctctcaaagc gcgataaatc agcaacggat gtgccattct ctaataaaaa tgccgggttg
    12481 aaataacttt ccgcatagct taaaaaggag ggtaaaatgg caagagatga ataacaaatt
    12541 ccggccagac accaggtata cctctgcgga atatccgtag tagtgggtat tttcataatt
    12601 agctctgata ttgtcagatg attcggttag tttaagttca cttctctgac cgggggtact
    12661 gaaccataat cattaacggt ctgaaatttc agctttccct gcactcctga gggtaaggga
    12721 atttgtgaat taactctggg tgccgccata gcattcccta acttttggct gcccacttgt
    12781 aagttaacca gggtgacata aaaaggtgat ggattatgaa cattgatata gttaccggat
    12841 cgcgaaaact tgagtgtgtc cggcgcttct gcgggtaatt cttgtaattg aattggacgg
    12901 agaaataact tcatgcgcga taaaatcgcc agttgcaaaa cattcctgcc gttcaatgca
    12961 tttttatcta ccgaagggat cgtcttaaca ttcatccaga acagagactc acgatctgcc
    13021 gccagcggtg gaccggtgta aataatacgc aaggtatttt cgcttttggg ttcactaaca
    13081 aacagtggcg gtgtaatgat gaatgacttt tcttttacac cgctgctgtt ttcaatccac
    13141 gaattgacca gaaagcgctc attggtatgg ctatttctaa tccataccgc agtctgttta
    13201 gcatcagcgg gataaataat acgcgtggca cctaatgcga tccctccggc agcatgggcg
    13261 ctggccgaaa tgattaaata aaatataatg agcatcaata actttatttt agtcatcata
    13321 gttaatacgc cttgtgtatt cctgacggca aaaataagaa atataaagaa atgattgctt
    13381 tggtttctta caacacatca ttcccttttc atttaataaa gcggcatgat acaaagtgaa
    13441 catgatttcg atttacattg ccaccatacc gcttcagaca accggtacat tactttgtta
    13501 taagacgtga tatataattc aaaacaacgt ggttttgatt attcgtatct cataataaaa
    13561 gtcgcgtcag catttgcttg ccctgctgat gcacttgtac cggtgccttt ataacgtgca
    13621 gaaaaatgaa gaacgttggt cccggggatc aagttctggt tggttgagaa gctattccca
    13681 tcaggtttga gaattgcgga tgtattatca agtatttcaa taccgacacc cgttgctgtt
    13741 gttgtattgg tactggatgc aatggccaat aaattatcat tgattgcatc agcctgacca
    13801 gaaaatgcaa cggcagcatt agcggcaaca acaggatcgc agtcgttcaa ctgaatggtg
    13861 aatggaatta atgcagaggt attaccaaca gcattgaaaa tatcggtacg atattgaccg
    13921 agtgtgacaa cctggtctgc cgagtcagta ttcactgaac aggcagcatt caccagttcg
    13981 ccttcaaagt gaattgtacc gccatctaca acggcagcat aactacccgt agcggcgaat
    14041 agtgcggcag ccagcgcaga cgaaataaat cttaatttca tatatatt
//
LOCUS       DAZKHC020000105        13946 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZKHC020000105 DAZKHC020000000
VERSION     DAZKHC020000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.105"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(34..738)
                     /locus_tag="ACHOSM_RS19015"
                     /old_locus_tag="pgaptmp_003803"
     CDS             complement(34..738)
                     /locus_tag="ACHOSM_RS19015"
                     /old_locus_tag="pgaptmp_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU4560216.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGVMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(875..1327)
                     /gene="moaE"
                     /locus_tag="ACHOSM_RS19020"
                     /old_locus_tag="pgaptmp_003804"
     CDS             complement(875..1327)
                     /gene="moaE"
                     /locus_tag="ACHOSM_RS19020"
                     /old_locus_tag="pgaptmp_003804"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HGU4560217.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1329..1574)
                     /gene="moaD"
                     /locus_tag="ACHOSM_RS19025"
                     /old_locus_tag="pgaptmp_003805"
     CDS             complement(1329..1574)
                     /gene="moaD"
                     /locus_tag="ACHOSM_RS19025"
                     /old_locus_tag="pgaptmp_003805"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HGU4560218.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1567..2052)
                     /gene="moaC"
                     /locus_tag="ACHOSM_RS19030"
                     /old_locus_tag="pgaptmp_003806"
     CDS             complement(1567..2052)
                     /gene="moaC"
                     /locus_tag="ACHOSM_RS19030"
                     /old_locus_tag="pgaptmp_003806"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HGU4560219.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2055..2567)
                     /gene="moaB"
                     /locus_tag="ACHOSM_RS19035"
                     /old_locus_tag="pgaptmp_003807"
     CDS             complement(2055..2567)
                     /gene="moaB"
                     /locus_tag="ACHOSM_RS19035"
                     /old_locus_tag="pgaptmp_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HGU4560220.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2589..3578)
                     /gene="moaA"
                     /locus_tag="ACHOSM_RS19040"
                     /old_locus_tag="pgaptmp_003808"
     CDS             complement(2589..3578)
                     /gene="moaA"
                     /locus_tag="ACHOSM_RS19040"
                     /old_locus_tag="pgaptmp_003808"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HGU4560221.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEVRISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3566..3710)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3975..4883
                     /gene="yvcK"
                     /locus_tag="ACHOSM_RS19045"
                     /old_locus_tag="pgaptmp_003809"
     CDS             3975..4883
                     /gene="yvcK"
                     /locus_tag="ACHOSM_RS19045"
                     /old_locus_tag="pgaptmp_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HGU4560222.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5075..7096)
                     /gene="uvrB"
                     /locus_tag="ACHOSM_RS19050"
                     /old_locus_tag="pgaptmp_003810"
     CDS             complement(5075..7096)
                     /gene="uvrB"
                     /locus_tag="ACHOSM_RS19050"
                     /old_locus_tag="pgaptmp_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGU4560223.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7675..8352)
                     /gene="bioD"
                     /locus_tag="ACHOSM_RS19055"
                     /old_locus_tag="pgaptmp_003811"
     CDS             complement(7675..8352)
                     /gene="bioD"
                     /locus_tag="ACHOSM_RS19055"
                     /old_locus_tag="pgaptmp_003811"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU4560224.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8345..9100)
                     /gene="bioC"
                     /locus_tag="ACHOSM_RS19060"
                     /old_locus_tag="pgaptmp_003812"
     CDS             complement(8345..9100)
                     /gene="bioC"
                     /locus_tag="ACHOSM_RS19060"
                     /old_locus_tag="pgaptmp_003812"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HGU4560225.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     SRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9087..10241)
                     /gene="bioF"
                     /locus_tag="ACHOSM_RS19065"
                     /old_locus_tag="pgaptmp_003813"
     CDS             complement(9087..10241)
                     /gene="bioF"
                     /locus_tag="ACHOSM_RS19065"
                     /old_locus_tag="pgaptmp_003813"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HGU4560226.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10238..11278)
                     /gene="bioB"
                     /locus_tag="ACHOSM_RS19070"
                     /old_locus_tag="pgaptmp_003814"
     CDS             complement(10238..11278)
                     /gene="bioB"
                     /locus_tag="ACHOSM_RS19070"
                     /old_locus_tag="pgaptmp_003814"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HGU4560227.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11365..12654
                     /gene="bioA"
                     /locus_tag="ACHOSM_RS19075"
                     /old_locus_tag="pgaptmp_003815"
     CDS             11365..12654
                     /gene="bioA"
                     /locus_tag="ACHOSM_RS19075"
                     /old_locus_tag="pgaptmp_003815"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HGU4560228.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMVLQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQK
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12713..13189
                     /locus_tag="ACHOSM_RS19080"
                     /old_locus_tag="pgaptmp_003816"
     CDS             12713..13189
                     /locus_tag="ACHOSM_RS19080"
                     /old_locus_tag="pgaptmp_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HGU4560229.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            13693..>13946
                     /locus_tag="ACHOSM_RS19085"
                     /old_locus_tag="pgaptmp_003817"
     CDS             13693..>13946
                     /locus_tag="ACHOSM_RS19085"
                     /old_locus_tag="pgaptmp_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspJ"
                     /protein_id="HGU4560230.1"
                     /translation="MPTIKNCLSSISNILRNEKTSYSLIKTEQTGKLLNRKITTTPTP
                     AKLLSYRNADLIKENHITEKVLSIFNIKRDFVAVRIQSNQ"
BASE COUNT         3322 a         3767 c         3506 g         3351 t
ORIGIN      
        1 acacatggcc gggatagtga tggtgaaaaa gaattaacgg cggttgccga agatccgtaa
       61 caacatcagg aacaggttga tgaagtccag atacaaggtt aacgcgccaa gaatggaata
      121 tttgcgcagg ttcgacgtgt cgcgggtatc aatctgctca cccatatttt tcaatttctg
      181 agtgtcatac gccgtcaatc cgacaaagac aatcacgccg atgtaggtaa ctgcccacat
      241 caatgcttcg cttttcagcc agaagttgac cagcgatgcc agcacaatgc cgattaacgc
      301 cataaacagc atattgccaa agccacttaa atcgcgcttc gtggtgtaac cgtacaggct
      361 cattacgccg aacatcccgg cagtaacgac gaaagtactg gcgatagaag cagcggtata
      421 gacaatgaat atactggaaa gcgtaagacc cgtcagcgcc gaataaagca taaagagcat
      481 cgtcgttaca cctgcgctca gcttttgaat catcgctgat aacacaataa ccaatgctaa
      541 ttgcgcgatg atcagaccga ttaaaaagac acggttagtg aacaacagct ccatcacggc
      601 cgcggaatta gccgcatacc aggcaacaaa tgcggtcagc aacaagccaa cggtcatcca
      661 gccatagact tgcgccatat aagtttgcaa gccagcccgg ggttgtacga ttgaatcaga
      721 acgtgggaat ctgtccatga cgatctcctg aagatataag gaatatctta aggatactgc
      781 aaaatgatga ggctgtgcat cgacgcagcg taaacgcatg tactgagcgg tgaaattgcc
      841 ggacgcagcg gtgccttatc cggctaacaa aaaactacca gcgttttgcc gcctgctgat
      901 cgctctcccg ggcttcaacc cagcggtcgc cttccggcgt ggcttcgcgc ttccagaacg
      961 gtgcgcgggt tttgagataa tccataataa actgcccggc ttcaaacgca ctgctgcgat
     1021 gcgcactggt gacaccgaca aaaacgattt catcgcccgg ccataattcc ccgatgcggt
     1081 gaatcaccgt gacgcgcccc agcggccagc ggttacgcgc ttcatcaacg atttctgcca
     1141 gtgctttttc agtcatcccc ggatagtgtt cgagggttaa tgctttgacg ctgtcaccca
     1201 ggttatggtt gcgcacctta ccggtaaacg tgaccactgc accgtcttcg tcacgctcag
     1261 ccagccacgg gtactcttct cctacgctga acggctgcgg gccgacaaca attttggttt
     1321 ctgccatctt aacctccggt taccggcggg aagaaagcta cttcgtcgcc gtcagtcagc
     1381 ggatggtcaa aactcaccag cgtctggttg acggcagcca gcaatttgcc atcttccagc
     1441 gccagcgccc agcgatcgct ctgcgcagcc aggtgctggc gtaacgcttc aacggttggg
     1501 taatccgcag ccacttcggt tgcatctgtt cccaccaact cgcgcacctg ggcgaaaaaa
     1561 agaactttaa tcatccgctt ccaccttaaa gtcacccgac ttgccgccgc tcttcgccag
     1621 caaacgtacc ggaccaatca ccatatcttt ttgcaccgct ttgcacatgt cataaatggt
     1681 cagcgccgcc acggaggccg cggttaacgc ttccatttcg acaccggttt tcccggtcag
     1741 gcggcataag gtttctatac gcacccgatt gtgctccggc tcggcctgta aattgacttc
     1801 aactttgctg agcatcagcg ggtgacacag cgggatcaga tcccaggtgc gttttgccgc
     1861 ctgaataccg gcaatacgcg cagtggcaaa tacgtcgcct ttgtggtggc gaccatcaat
     1921 aatcatcgcc agcgtctcgc tgcgcatggt gacaaaggct tccgcccgcg cttcacgcac
     1981 ggtttccgct ttggcggaga catccaccat gtgcgcttcg ccagcggcgt tgatatgggt
     2041 cagttgcgac atacttattt cttcaaatgt ggatggaaat tacacggacg cgtacgggca
     2101 tccagctgcg gcgcgatgat attttcccat gcggtacggc acgctttggt cgaacccggc
     2161 atggcgaaaa tcagcgtttt gttggcgacg cccgctaccg cacgagattg caacgtggaa
     2221 gtgccaatct cttcaaacga caacatccgg aacacttcac caaaaccttc aacttcacgg
     2281 tcgaacaacg gcagcaatgc ttcgggagcc tgatcacctt ccgtcaggcc agtccccccc
     2341 gtaatcaaca ccacttgtac atcgtcgctg gcgatccacg cagatacctg agcgcgaata
     2401 gcgtagcggt tttctttcac aatggcttta tcgacaacgt gatggcccgc ttcttgcgcc
     2461 gaatcgcgca aatagtgacc ggaggtatcg tcttcttcac cgcgacgatt agaaaccgta
     2521 agaatagcaa tacgggtcgg gataaattca gtgcttacct gactcatctg atctctcctt
     2581 ttgacgtttt agccgccaat gtacgataag ttttgcgtaa taccggtgtt gttttgatgc
     2641 aggaaatggg tctgtttctt ctcccgcagc gccgctgaaa tacgcacttc cagcgcctgt
     2701 tgctgggtat cgtcttccag cagatcgcgc agattaacgc cgccttcacc aaacaggcag
     2761 agatggagtt taccaatgga ggaaacgcgc aggcggttgc aagtggcgca gaagtctttt
     2821 tcatacggca tgataaggcc aatctctccg gcgtaatccg gatgacaaaa gacttgcgcg
     2881 ggaccgtcgc tgcgttgacg taattggtgg atccagccgc gacgcagtag ctcgtcacgc
     2941 agaacctgac cagagatgtg atgcttacgg aagagctcgc tgccctcgcc cgtttccatc
     3001 agttcgatga aacgcagctg gatagggcga tgctggatcc agttcagaaa ggtgtcgagc
     3061 tggtgatgat taacatcacg catcagcacg gtattgactt tgaccttctc aaaaccggcc
     3121 tcaaatgcag catcaatccc tgccatgacc tggttgaatt tatcctgccc ggtaatagcg
     3181 tgaaactggc gggcgtccag actgtcgaca ctgacgttaa tgccagtaag tcccgcatcg
     3241 cgccagttcg ccacatcgcg ttccagacgg taaccattgg tggtgaccgc aatctggcgg
     3301 atagcgtcgt tttcccgcac agcggcgatg atatcggtaa agtcgcggcg taaagacggc
     3361 tcacctcctg tcaggcgcac tttttcggtg cccagactgg cgaaggcgcg cgtaacccgg
     3421 cgaatttcat cgacggtaag aaagccttta ttggtgacgc cgctcggttt gtagccatcc
     3481 ggcaggcagt aggtgcaacg aaagttacac acatcggtaa tcgacaggcg caagtagtaa
     3541 aacttacgcg caaatgcatc agtcagttgt gaagccatgt acacctttcc agatacggga
     3601 ggcgaagtca tttcttcctt cgccctggtg gcaatcttta cgagcattgt cacggccaaa
     3661 gcaccgtatc agttgaccca ggtgcagagg ctagagtgtt tagtggttaa gccgatacta
     3721 gcgtgtaaat gagattttta ccatccacac tctcgctatg taatcatgta tatagcgtca
     3781 tgatcgtgct atttcgctac agaataaggg aaaatggcgg ttttactttg atatacatca
     3841 tcagggaggc atagcgtgca tcgtccttta gaggtagaag aaaatagttt gtatcctcaa
     3901 tattggcagg ttaattgctg tttccccgca atttgcgcta ctgtatcgcg gataatctga
     3961 ctccaggaaa ctatatgcgc aatcgtacgc tggctgatct tgatcgtgtc gttgctctcg
     4021 gcggagggca tggactggga cgcgttctct catcactttc gtctttgggt tctcgtttaa
     4081 cgggtatcgt caccaccacg gataatggtg gctcgacggg gcgtattcgc cgttcagaag
     4141 gcggcattgc ctggggcgat atgcgcaact gcctcaacca actgataacg gaaccgagcg
     4201 tcgcctccgc gatgtttgaa taccgttttg gcggcaatgg cgaactttcc ggtcataatc
     4261 tcggaaactt gatgttaaag gcgctggatc accttagcgt gcggcctctg gaagccatca
     4321 atttaattcg taatctgctg aaagtggata cgcatttgat tccaatgtca gagcatcccg
     4381 tcgatctaat ggcgattgac gatcaggggc atgaagttta cggcgaggtc aatatcgacc
     4441 agttaactac gccaattcaa gagttattat taacgccgaa tgtacccgca acgcgtgagg
     4501 cggttcacgc tatcaatgaa gcggatctca tcattattgg gcctggcagt ttttatacca
     4561 gcctgatgcc aattctgctg ctgaaggaaa tcgcccaggc attacgccgc acgccagcgc
     4621 cgatggtcta tatcggcaat ctggggcgtg agttgagttt acctgcggct aatttgaagc
     4681 tggaaagcaa gctggcaatt atggagcagt atgttggtaa aaaggtcatt gatgcggtca
     4741 tcgtcgggcc aaaagtggat gtctcggcgg tgaaagagcg gattgtgatc caggaggtac
     4801 tggaggccag cgatattccg tatcgtcatg accgccagtt gttacataac gcgctggaaa
     4861 aggcgttaca ggctttaggt taacggaaat ccgaaggaaa attccggctt cctattgaag
     4921 acaaagtgcg cgttgtttat gccggatgcg gcgtgaacgc cttatccggc ctacaaaccg
     4981 cgcaaattca atatattgcg gagaaaatgt aggcctgata agcgtagcgc atcaggctgt
     5041 tttccgtttg tcatcagtct tcttcgctat cctgttacga tgcggcaata aacaactcac
     5101 gcaactgatg caactggtca cgaatttgtg ccgcttcttc gaactccaga ttctgcgcgt
     5161 gttgcatcat cagcccttcc agttcatgga ttttctgctg caacgctttc ggcgacatat
     5221 ccatcggcac attatccggc tcaacaatcg ggcgcgattt tcctctgccc ttcgctttgg
     5281 ttttggcaat gttctgcccc agcgccagga tatcgaccac tttcttgttc aagccttgcg
     5341 gcgtaatgcc gtgttcctcg ttgtacttct gctgtttctc acggcgacgt tcggtttcac
     5401 caatcgcttt cgccattgat ggagtgatct tatcgccgta gagaatcgct ttaccgttaa
     5461 cgttacgtgc cgcacgacca atagtctgga tcaacgaacg ttcggaacgc aggaagcctt
     5521 ctttgtcagc gtcgaggatc gccaccagcg acacttccgg catatccaga ccttcgcgca
     5581 gtaagttgat ccctaccagc acgtcgaact cacccagacg caagtcgcgg ataatctcca
     5641 tacgttcgac ggtgtcgata tctgagtgaa gataacgcac gcgctcgcca tgttcttcga
     5701 gatattcggt aagatcttcc gccatccgct tggtcagtgt ggtgaccagt acgcgttcgt
     5761 taattgccgc tcgctgacga atctccgaaa gaagatcatc aacctgtgtc gccaccggcc
     5821 gcacttcgat aatcgggtca agcaatccgg ttggacgcac cacctgatcc accacatcgc
     5881 cgccggattt ttccagctcg taattacccg gcgtcgccga aacatagatg gtttgcggcg
     5941 ctaatgcttc gaactcttca aacttaagcg gacggttatc cagcgcagat ggcaggcgga
     6001 agccgtactc caccagtgtc tctttacgcg cccggtcacc gcgatacatg ccgccaattt
     6061 gtggaatggt gacatgagat tcatcgacca ccagcagccc atcggcaggg aggtaatcaa
     6121 acagcgtcgg cggtggctca cccggtccac gaccggagag gaagcgcgag tagttttcaa
     6181 tccccgaaca gtagcccagc tcgttcatca tctccagatc aaactgggta cgctgggtca
     6241 gccgctgctc ttccagcagt ttgttgtttt ccaatagcac tttgcgtctg gcggccagct
     6301 cttctttgat ctcctccatc gcctgtacga tgcgctcgcg cggtgtgacg tagtgcgttt
     6361 tcgggtagat ggtaaaacgt ggaatggtgg aaacaatctg cccggttagc gggtcaaata
     6421 acgacaatcg ttccacttcc tcgtcaaaca gttccacgcg aagtgcaatg tcatccgatt
     6481 ctgccgggaa gatatctatc acctcgccac gaacgcggaa agtaccacgc tggaatgctt
     6541 gatcattacg agcgtattgc agctccgcca gtcggcgcag aatcgcgcgc tgatcgataa
     6601 tcataccgac cgtgagatgg agcatcatct tgagatataa atcaggatcg cccagaccat
     6661 aaatcgcgga aacagaggcc actacaacca catcacgccg ctccagcatc gctttggtgg
     6721 cggataaacg catctgctca atatgttcgt taaccgaggc atctttctca atgaaagtgt
     6781 cggaactcgg tacataggct tccggctgat agtagtcgta gtaggagacg aaatactcca
     6841 ccgcgttttc cgggaagaac tctttcattt cgccatacag ctgggccgcc agcgttttgt
     6901 tgggcgcaag taccatggtt gggcgctgaa ggtcagcaat gacattggca atggtgaatg
     6961 ttttcccgga gccagtcacg ccaagtaacg tctggtgcgc caggccatct tccagcccct
     7021 cttcgagacg tcgaatcgcc tctggctgat cgccagaagg tttaaaagcg gaattcaatt
     7081 tgaacggttt actcatgagt cgctacctga aggagttggg cgggcaggta tgtaatttta
     7141 ctcgtcgtac ttaattatgc caacaaatta tactggataa aaaaacagtt catcaccata
     7201 atatttctgt tacagcgtaa actccgctgt caatcatgag caagatttac tctgtggcga
     7261 gataaaactc cggccttacc gggttatccc caaagcaacg gcttttttaa catttgtcaa
     7321 gatgagtgat gacagtttta tggcaagaga tgcctgttca gtgactgcca ttgcaattat
     7381 ctaaccaatt aaaaaataaa agatatttct ttgagccgtc tttaacgcca attcgcgtgc
     7441 aagccgcgta ttctctcgct tgcctcgtgt tttctaactc taatacacat ggttatccac
     7501 aggaatagtg gataactgtc tgcagcccct atcccccgcc gcctgggcaa ttcccacatt
     7561 caccgcatca tgtggaggta aaattttttt cgtgattatt ttagaattta attggttaaa
     7621 ttgcagtcaa tcgaagactc gatctcgctc gcaatttaac caaatacagg atggctacaa
     7681 caaggcaagg tttatgtact ttccggttgc cgcattttct ggattttctg caagccaggg
     7741 gatctctccc agcagcggcg cgggaatcat gcgggtgagc gtggtcatat attcagcgtg
     7801 acgttttccc ggaggcgtaa catcgttcgc cacccaaccc gccagagtca gtccggcgtg
     7861 ttgtattgcc tgcgcagtca acatcgcgtg attaatacag ccgagtttca caccaactac
     7921 cagtatcacc ggcagttgtt cctgtgttac ccaatctgca aaagtgaaag tgtcagaaag
     7981 cggcgtaaac cagccgccag caccttccac taacacccag tcagcctgtt gttcaagcgc
     8041 gcgtaatccg gcgctcatta ccaatgattc tatcggcctg ccctcttgcg cgctgatgat
     8101 gtgcggcgag gtgggttctg cgaaggtgta aggatttacc gttgcgtaat ccagctgcag
     8161 gctgctgttg tgctgtaacg ccagcgcgtc actattacgt aagccttccg gggtcttttc
     8221 gctgccagag gcgaccggtt tataacctgc cgtccgataa cctactgcct tcgcggcttg
     8281 taaaagtgca caactggcga cggttttccc cacttcggta tccgttccgg tgacaaaata
     8341 acgtttactc acgagcaatc actcccaaaa aaagatgata cgtcagagga tatcgcccct
     8401 gttgttgcgg ccaggccagt tgcaatcgct gcaattgcga acgcgataat attcgcgggt
     8461 cgcgcccttc atgaagatgc gtagcaccga tgcctttcag cgaacgcatg gcactgagcg
     8521 catcatcaaa ccatagtgtg atgggctgaa tatgatgttg ataatgcacg ccgtttagcg
     8581 actgttcgat ttcatctggc ggtaaaaagc gattagcatg cggacgctcg tccaccgcct
     8641 gccacgcctg atgcagttcg ggtaacgatc cctgcaccag cgtggtaaac gccaccacgc
     8701 ctccggggcg caccacccga tacagctcgc ggagtgccgt ggataaatta ccgcaccact
     8761 gcaccgcgag attgctccat gcaagatcga acgtcgcagt cgctaacggc agggattcga
     8821 tatctcccgc cagataatgg tctgcagcat ctttctggcg ggcttgcaca agcattggcg
     8881 gtgagagatc taacgccgtc acctgtgcgt gacgctctcg ccagtggcgg ctcatccagc
     8941 ctggcccaca ccccgcgtcc agtacgtggg tgtatttacg ctgtgggagc attgccagta
     9001 agacgtcagc actccggcgc tgtaactctg cgtgttgctc atagtgcgcg gctgcccgac
     9061 caaatgccgc tgcaatggct tgtttattaa ccgttgccat gcagcacctc cagcagacgg
     9121 tcgatatcct gcatttcatg cgctgcggtt agcgttaagc gcagtcgcgc agtaccagcg
     9181 ggtacggttg gcgggcgaat cgccgtgacc cagcagcctt gctggcgcag tttttctgcc
     9241 agttgtaacg cacggctgtt atcaccgacg atcaatggct ggatggcgct gcatgaatca
     9301 gcaagcgtaa acggcaaatc ctgtactccg gcacgaaaac gcgcaatgag tgacaccagt
     9361 ttttcgcgcc gtgcatcccc ctcatcacgg cgaatgaccg ccagcgacgc acgtaatgcc
     9421 tgcgcctgag cgggcggcat actggtgctg tagataaggt ggcgggcgaa ttgcagcaga
     9481 taatccgcca ccgtactgga gcaaagcacc gcagccccac tgacgccaaa tcctttgcca
     9541 aaagtcacca ccagcaattc tggttttacc ttttgcagcc agcagctgcc gcgcccctgc
     9601 tccccgataa cgcccgtgcc gtgggcatca tcgaccatca accagccatt gtgctgttgc
     9661 gttacctgct ggatttccgc cagtggcgcg ctatcgccgt ccatgctgaa cacgccttct
     9721 gtcaccacca gttgctgccc cggacagggg gaagcaagca gtcgcgccag atgagcgaca
     9781 tcgttatgag taaaacggcg aagctgcgac gggcttaaac tggcagcctc cagcaaggag
     9841 gcatggctaa gccggtcggc aacaatacgg tcctctttcg ccatcatcgc ggcaatgact
     9901 gcctgattcg cggcaaaacc agagataaac agcagcgccc gcgaatagcc aagccactcg
     9961 gccagttctt cttccagcgc ctgatgcacc acgctataac cgctgacatg cccggaaccg
    10021 ccgctaccta caccaaactt ttctgcgccc tgctgccagg cgcggataat ttgcggatga
    10081 tggcttaaac ctaaataatc gttactggaa aagttcagat actggcgatc atccgccacc
    10141 agccagcgtc cggctccttg cgccaccgga taacggcgac gcagggcatc ggcagtacgc
    10201 cgcgcatcga gcgccgcgtt gattttctcc tgccagctca taatgctgcc gcgttgtaat
    10261 attcgtcggt gtccggggtc atcagcgcct gttcaagacg ttgctgttgt tcgttatccc
    10321 ctgccagcac ggcagtttgc tgcggattta gccccagttt gcggaacagt tgcaggtctt
    10381 tatcttcttc cggattcggc gtggtcagca gtttgcaacc gtagaaaatc gagtttgcgc
    10441 cagccataaa gcacatcgcc tgagtctgtt cattcatctg ctcgcgtccg gcagaaaggc
    10501 gcacgtaaga ggtcggcatc atgatccgcg ctaccgcaat agtgcgaata aaatcaaagg
    10561 catcgacatc atcgttatcc gccagtggcg tgcctttcac cttcaccagc atgttgattg
    10621 gtacgctttc cggcggcgtc ggcaggtttg ccagttgcag caataatccg gcgcgatctt
    10681 ttaccgtttc gcctaagccc acaatgccac ctgagcagac tttgatcccg gcatcgcgca
    10741 ctttttccag cgtatcgagg cgttcctgat aagtgcgtgt ggtgatgata ttgccgtaaa
    10801 actccggcga ggtgtccagg ttgtggttgt agtaatccag cccggcgttc gcgaggcgct
    10861 gcgcctgaga ttcactcaac gtgcccagcg tcatacacgc ctccagcccc atcgctttta
    10921 ccccctgcac catttgttcc aggtacggca tatcgcgttc gtggggattc ttccacgccg
    10981 cgcccataca gaagcgcgtc gatcctgccg ctttcgcttt gcgcgccgac tccagcacct
    11041 gttcaacttc catcaaccgc tcggcttcca gcccggtttt gtagcgcgag ctttgcgggc
    11101 agtatttgca atcttccgga caagctccgg tcttaatcga cagcaacgtg ctgacctgca
    11161 cctgacgagg atcgaaatgc tggcgatgca cctgctgcgc ttcaaacagc agatccagca
    11221 acggtttttc aaataattct gtgacttgcg acaatgtcca gcgtgggcgg tgagccatgg
    11281 ggcttctcca aaacgtgttt tttgttgtta attcggtgta gacttgtaaa cctaaatctt
    11341 ttcaatttgg tttacaagtc gattatgaca acggacgatc ttgcctttga ccaacgccat
    11401 atctggcacc catacacatc catgacctcc cctctgccgg tttatccggt ggcgagcgcc
    11461 gaaggttgcg agctaatttt gtctgacggc agacgcctgg ttgacggtat gtcgtcctgg
    11521 tgggcggcga tccacggcta caatcacccg cagcttaatg cggcgatgaa gacgcaaatt
    11581 gatgccatgt cgcatgtgat gtttggcggt atcacccatg cgcccgccat cgagctatgc
    11641 cgcaaactgg tggcaatgac gccgcaaccg ctggagtgcg tttttctcgc tgactccggt
    11701 tccgtagcgg tggaagtggc gatgaaaatg gtgttgcagt actggcaagc caaaggcgaa
    11761 gcgcgccagc gttttctgac cttccgcaat ggttatcatg gcgatacctt tggcgcgatg
    11821 tcggtgtgcg atccggacaa ctcaatgcac agcctgtgga aaggctatct gccagaaaac
    11881 ttgtttgctc ccgccccgca aagccgcatg gatggtgaat gggatgaacg cgatatggtt
    11941 ggttttgccc gcctgatggc ggcgcatcgt catgaaatcg cagcggtgat cattgagccg
    12001 attgtccagg gcgcaggcgg gatgcgcatg taccatccgg aatggttaaa acgaatccgc
    12061 aaaatatgcg atcgcgaagg tatcttgctg attgccgacg agatcgccac tggatttggt
    12121 cgtaccggga aactgtttgc ctgtgagcat gcagaaatcg cgccggacat tttgtgcctc
    12181 ggtaaagcct taaccggcgg cacaatgacc ctttccgcca cactcaccac gcgcgaggtt
    12241 gcagaaacca tcagtaacgg cgaagccggc tgctttatgc acgggccaac ttttatgggc
    12301 aatccgctgg cctgcgcagc agcaaacgcc agcctggcga ttctcgaatc tggcgactgg
    12361 cagaagcagg tggcagctat tgaagtacag ctacgcgagc aactggcacc cgcccgtgat
    12421 gccgaaatgg ttgccgatgt gcgcgtactg ggggccatcg gcgtggtcga aaccactcat
    12481 ccggtgaata tggcggcgct gcaaaaattc tttgtcgaac agggggtctg gatccggcct
    12541 tttggcaaac tgatttacct gatgccgccc tatattattc tcccgcaaca gttgcagcgt
    12601 ctgaccgcag cggttaaccg cgcggtacag gatgaaacat ttttttgcca ataacgggaa
    12661 gtccgcgtga gggtttctgg ctacactttc tgcaaacaag aaaggagggt tcatgaaact
    12721 catcagtaac gatctgcgcg atggcgataa attgccgcat cgtcatgtct ttaacggcat
    12781 gggttacgat ggcgataata tttcaccgca tctggcgtgg gatgatgttc ctgcgggaac
    12841 gaaaagtttt gttgtcacct gctacgaccc ggatgcgcca accggctccg gctggtggca
    12901 ctgggtagtt gttaacttac ccgctgatac ccgcgtatta ccgcaagggt ttggctctgg
    12961 tctggtagca atgccagacg gcgttttgca gacgcgtacc gattttggta aaaccggata
    13021 cgatggcgca gcgccgccga aaggcgaaac ccatcgctac atttttaccg ttcacgcgct
    13081 ggatgtagag cgtattgatg tcgatgaagg agccagcggc gcgatggtcg ggtttaacgt
    13141 tcatttccac tctctggcaa gcgcatcgat taccgcgatg tttagttaat cactctgcca
    13201 gatggcgcaa tgccatctgg tatcacttaa aggtattaaa aacaactttt tgtcttttta
    13261 ccttcccgtt tcgctcaagt tagtataaaa aagctgaacg tgaaacgcca aagaccatta
    13321 atatcaatgt gttacagtat atttggtcta aaaaatagac tgcataatac tacaaaacac
    13381 aacatatcta gtaactatga atcaactact tagatagtat tagtgacctg agacagagca
    13441 ttagcgcaag gtgattttat gtcttcttgc gctaattttt tgtcataaaa acatgtggca
    13501 gactttgtat atctctgctt tgccacgcca caccatcatg cattatggct atgcctaaac
    13561 agaatcaata agaatagaac ataaaaaatg acaactctac tagacaatta actacaaaaa
    13621 tgatagcaat tagtttatat aatataagac ttcctatcaa aagttcatcc atatgaagac
    13681 aaggaattta ttatgccaac cataaagaac tgcttatcat caattagtaa catattacgc
    13741 aacgaaaaaa catcatattc tctaataaaa acagagcaaa caggaaaact gttaaacaga
    13801 aaaataacca ccactcccac accagcgaaa ctattatcat atcgaaatgc agatttaata
    13861 aaagaaaacc acattacaga aaaagtttta tctattttca atataaaacg ggatttcgtt
    13921 gctgtaagaa tccaaagtaa tcagtt
//
LOCUS       DAZKHC020000106        13435 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZKHC020000106 DAZKHC020000000
VERSION     DAZKHC020000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.106"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            90..920
                     /gene="yjiC"
                     /locus_tag="ACHOSM_RS19090"
                     /old_locus_tag="pgaptmp_003818"
     CDS             90..920
                     /gene="yjiC"
                     /locus_tag="ACHOSM_RS19090"
                     /old_locus_tag="pgaptmp_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGU4560231.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDSELTRKHERARMDTENIY
                     LSPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTETCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITSGPGCNIPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYLGTAEIHAKMGYKVIPGDEDAKLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1061..1834)
                     /gene="uxuR"
                     /locus_tag="ACHOSM_RS19095"
                     /old_locus_tag="pgaptmp_003819"
     CDS             complement(1061..1834)
                     /gene="uxuR"
                     /locus_tag="ACHOSM_RS19095"
                     /old_locus_tag="pgaptmp_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HGU4560232.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSQNTDGPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2049..3509)
                     /gene="uxuB"
                     /locus_tag="ACHOSM_RS19100"
                     /old_locus_tag="pgaptmp_003820"
     CDS             complement(2049..3509)
                     /gene="uxuB"
                     /locus_tag="ACHOSM_RS19100"
                     /old_locus_tag="pgaptmp_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HGU4560233.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGKAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GVFADDLPKNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3590..4774)
                     /gene="uxuA"
                     /locus_tag="ACHOSM_RS19105"
                     /old_locus_tag="pgaptmp_003821"
     CDS             complement(3590..4774)
                     /gene="uxuA"
                     /locus_tag="ACHOSM_RS19105"
                     /old_locus_tag="pgaptmp_003821"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HGU4560234.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5114..6457
                     /gene="gntP"
                     /locus_tag="ACHOSM_RS19110"
                     /old_locus_tag="pgaptmp_003822"
     CDS             5114..6457
                     /gene="gntP"
                     /locus_tag="ACHOSM_RS19110"
                     /old_locus_tag="pgaptmp_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HGU4560235.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYSVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6630..7532)
                     /gene="fimH"
                     /locus_tag="ACHOSM_RS19115"
                     /old_locus_tag="pgaptmp_003823"
     CDS             complement(6630..7532)
                     /gene="fimH"
                     /locus_tag="ACHOSM_RS19115"
                     /old_locus_tag="pgaptmp_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU4560236.1"
                     /translation="MKRVINLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7552..7995)
                     /locus_tag="ACHOSM_RS19120"
                     /old_locus_tag="pgaptmp_003824"
     CDS             complement(7552..7995)
                     /locus_tag="ACHOSM_RS19120"
                     /old_locus_tag="pgaptmp_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4560237.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNSG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8068..8598)
                     /gene="fimF"
                     /locus_tag="ACHOSM_RS19125"
                     /old_locus_tag="pgaptmp_003825"
     CDS             complement(8068..8598)
                     /gene="fimF"
                     /locus_tag="ACHOSM_RS19125"
                     /old_locus_tag="pgaptmp_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HGU4560238.1"
                     /translation="MRNKPFYLLCAFLWLAVSHAFAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8608..11244)
                     /gene="fimD"
                     /locus_tag="ACHOSM_RS19130"
                     /old_locus_tag="pgaptmp_003826"
     CDS             complement(8608..11244)
                     /gene="fimD"
                     /locus_tag="ACHOSM_RS19130"
                     /old_locus_tag="pgaptmp_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HGU4560239.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTYYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11311..12036)
                     /gene="fimC"
                     /locus_tag="ACHOSM_RS19135"
                     /old_locus_tag="pgaptmp_003827"
     CDS             complement(11311..12036)
                     /gene="fimC"
                     /locus_tag="ACHOSM_RS19135"
                     /old_locus_tag="pgaptmp_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU4560240.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12073..12612)
                     /gene="fimI"
                     /locus_tag="ACHOSM_RS19140"
                     /old_locus_tag="pgaptmp_003828"
     CDS             complement(12073..12612)
                     /gene="fimI"
                     /locus_tag="ACHOSM_RS19140"
                     /old_locus_tag="pgaptmp_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4560241.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12677..13231)
                     /gene="fimA"
                     /locus_tag="ACHOSM_RS19145"
                     /old_locus_tag="pgaptmp_003829"
     CDS             complement(12677..13231)
                     /gene="fimA"
                     /locus_tag="ACHOSM_RS19145"
                     /old_locus_tag="pgaptmp_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4560242.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAETTPTTVNGGTVHFKGEVVNA
                     ACAVDAGSVDQTVQLGQVRTASLKQTGATSSAVGFNIQLNDCDTSVATKAAVAFLGTA
                     IDSAHPKVLALQSSAAGSATNVGVQILDRTGAELTLDGATFSAQTTLNNGTNTIPFQA
                     RYYAIGEATPGAANADATFKVQYQ"
BASE COUNT         3266 a         3480 c         3229 g         3460 t
ORIGIN      
        1 tgcatgtcat ttgtttactt ctctttttac tacagaaaaa tttatctatt tttctaagca
       61 tcagtgaaat actcataaag gtaactatta tgtctatgcc attaagcaat gcccttcaga
      121 gtcaaattat tacagataat cacttccttc atcatccaaa agtcgacagt gagttaacac
      181 gtaaacatga acgtgcacgc atggatacgg aaaatatcta tctttcgcct cttgctcgtg
      241 gtaataacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt gatattagta
      301 aagagtcctg gccatttaat tatgtgacag aaacatgcag agaattcaat ggtattacaa
      361 caacaggacg tatgctctat cgaaacttga aaatcacctc agctttagac gaaatatatg
      421 gtggtatatg taaaaaggcg catgctgcca cagagttagc agaaggatta cggctaaatt
      481 tatttatgaa aagccctttc gatcctgttg aggactatac agttcatgaa ataacatcag
      541 gccctggctg caatattcct ggttatgccg gaaccacgat aggatatatt tcaacacttc
      601 cagtttctca ggcaaaaaga tggacaaatg agcagccaag gattgatatc tatattgatc
      661 agataataac tgtctcaggg gtggcaaatt cttccggatt cgcccttgcc gcattactta
      721 atgctaatat tggaatggga aatgacccaa taattggtat agaagcctat ctaggtaccg
      781 cggagatcca tgcaaaaatg ggatataaag ttatccccgg tgatgaagat gcaaaactca
      841 aacgtatgac tctgcaaccg tcatctcttc cagagctgtt tgaattaaaa aatggtgaat
      901 ggaattatat tggtaaataa ccactgagaa gaatgagtac tcacgttcag gttatacaga
      961 tagttaaaca ataccctgca atttagaaag taatactcat tgcgacttct taatttaaga
     1021 aagcgcaagg aatgtttacc cttgcgctta tcataataag tcaggcgtcg actttatcca
     1081 gcggccatga atcaaacaga tagccgtcaa aataaatatc gtcaacgtta gagaattcca
     1141 gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc gctcgggcat
     1201 cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct ttgcgataca
     1261 ggctgtcatc cagatggctg tgcaactgga tccacatcgg attgttttcc cgccactgcc
     1321 aggactgacg gaacagctcc accagcatgc tgttatgcgt cgcttcggca atggcgagat
     1381 ggaactgcat gtcaccactt tcgctgctgc ccggcgcgct ggaagccagt tcacgctctt
     1441 ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc agggcggcaa
     1501 attccgcgat gttactttcc agtaactgac gcgcctgtaa cagctcaaaa ggaccggcat
     1561 cattgcagac gttggcatcc ggaccgtctg tgttctggct gcctgagcta tcaagaacat
     1621 agataccggc accccgacgc acttccacca gccctttgat ctccagcatg atcagcgctt
     1681 cacgtaccac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc ggcggcagcc
     1741 gttcgccagg attgtacggc gtctttatga tcagatcgcg gatcatcgcc ccgacttcct
     1801 ggtaaggtct ttgcgcagag gtggcagatt tcatggtgtt gtgtccgtta gatgcggtta
     1861 atcaaatttt tgccggatgc ggcgtgaatg ccttatacgg cctacaacgg gcaattttac
     1921 ggcacccgtt aatctcaaac tataccatta gcactggaag ttggcacgta caggtgggta
     1981 aaaaaacgaa aactggtcaa ccaaatatgc gtttgattga ccagtaggtc tgtagggtaa
     2041 tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcacacag ctgctgatat
     2101 gccgccgtca ccgcgccaac aaagtcggca ttcttcggca gatcatcggc aaagacacca
     2161 ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt gatcttctgg
     2221 aactccgcca gcatcgggtc gaccacgtca atagctttac cctgctcatc cacgccctgg
     2281 gtgtaacgca tccagccagc cacgcccagc gccagatgac gccagctacc gccgttttgc
     2341 aggtgcagac gcaccggatc cagcagacgc tgcggtaact tctggctgcc gtccatcgca
     2401 atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag cagcgtcgca
     2461 taggcgttca ggtctgtacc ttccggcatc gacagcgttg gcgcttgttc ctgcatcatc
     2521 agggcaaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat agtttcatag
     2581 ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc cgttcagcat acgcagcttc
     2641 atcatttcga acggcacaac gtctgcaaca aactgtgcgc ccactttatc ccagtccggg
     2701 cgaccattaa cgaagttatc ttcaatcacc cactgacgga acggttcgca ggcaatggcg
     2761 cacgggtcgt aaacacccag ctggtcagca atttcctgta aggtttctgg cgtcgccgcc
     2821 ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat ccatgccgcc
     2881 agctgtggat cacgcgcctg agccagcccc agaaccgcga ccttcgccac atgaccgttt
     2941 tcacgcacgt tgtcgcagga catcaccgta aacgctttca gccctttttc acgacgcaga
     3001 cgcagggctt cgacgatgta accaatcgca gacttcggcg cagtcgggtt ttccagatcg
     3061 tgcttgatca gcgggttatt gagatccaac tgaccgcttg ccgcatcagc gcagtagcct
     3121 ttttccgtga ccgtcagaga gacaatcgcc gtctgcggac gcgccatcgc gttgagaata
     3181 ccttcgcagc cgtcgatttc cggatgcagc gcttctttca tcgaaccgat aattttcagc
     3241 tcggtgcttt ccgcgccttt ttccgccacg gtgtacagca gttgctgttt tttcaggttt
     3301 tcgatcagca cgcggtcgtt gcctggcatc aggttaactt cgcagatacc ccagtcgctg
     3361 tcggtacttt ccagcagatg atgggtatac agcgcctggt gcgcgcggtg aaacgccccg
     3421 caaccgagat gcacaatgcg tgattccaga cgagaatgat cccatgacgg gcgggcaacc
     3481 ggcagattgc tgtcaacaat agtagtcatc gtaaactcca gaatccgtct tcagagagtg
     3541 acggcaagga agaaggaacc ggcacggcgc gcagccatgc cggtggatat taacggctaa
     3601 agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc ggccaggcct ttcagacgac
     3661 caattgcgga gtaacctggg ttggttttct tcttcaggtc gtccagcatc tgatgaccgt
     3721 ggtccggacg catcgggatc aggtcttctt tgccttccgc tttacgacgg tgttcttctt
     3781 caacaatcgc tttcaccact tcgtacatat caacgtcacc gttcaggtgc gccgcttcgt
     3841 ggaaggtttt cgggttatct tcacgcatgg tggagcgcag atgggtgaag taaatacgcg
     3901 gaccgaactg cttgatcata tcaaccagat cgttgtcagc acgcacgccg taggaaccgg
     3961 tgcacatggt gaaaccgttc gccatgctgt ttacggtatc aaccatccac tgcatatctt
     4021 caatggtgga aacaatgcgc ggcaggccga ggatcgggcg cggcggatcg tccgggtgaa
     4081 cagccatacg gacgccgact tcttcggcaa ccggaataat cgctttcagg aagacagcga
     4141 agttttcgcg tagcttcgct ttatcgatat ctttgtacag ctccaggtgc ttacggaact
     4201 ggtcgagggt atacccttct tccgcacctg gcagaccggc aatgatgtta cgggtcagac
     4261 gcgctttgtc ttcgtcgctc atagtggcga agcgaacagc tgcctgagca atttcttctt
     4321 cggtgtaatc cgcttccgcg ccaggacgtt tcaggatatg catttcgaat gcagcgaatt
     4381 cgatctggtc gaagcgcaga gctttggagc cgtctggcag cacgtattcg aggtcagtac
     4441 gggtccagtc gagcaccggc atgaaattgt agcacacggt gcgaatgccg cactgcgcca
     4501 ggttgcgcag agtctgctga tagttagcga tccactgctc gtagttgcca gtgtgggttt
     4561 tgatatcttc gtgaattggc acgctttcta cgacagacca taccaggcct gcgtcttcaa
     4621 cgatcgcctt gcgtttgagg atctcttcta cggaccatac ttcgccgttc gggatatggt
     4681 gcagcgcggt aaccacgcca gttgcgcccg cctgacggac atcagctaaa gaaaccggat
     4741 cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt tcctctttca catagtgggt
     4801 tccacaacaa agagacgcgg aaccctggag ttgtttaggt atccggatga acaaccagac
     4861 cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt tctacctttt ggtaagacat
     4921 gtcaacccga attctgaaat tggttaacca catcacaaga atttcactct cctggttgtt
     4981 gatgcaaatt gcagcaaagg gtaatcgatg gttattgatc agaggatgtg atattcatcg
     5041 caacaatggt tgaccaattc acataacatg tcgcccaaaa taacaacggt tcaaccaggt
     5101 aaggtaaaac gttatgcatg tgcttaacat tctctgggta gtattcggta tcggcctgat
     5161 gctggtactg aatttgaagt tcaaaatcaa ttcaatggta gccttgttgg tggcggcgct
     5221 gtccgtcggg atgctggcgg gcatggattt gatgtcgctg ctgcacacca tgaaagcggg
     5281 cttcggcaac acgctggggg aactggctat catcgtggtg ttcggtgcgg tcatcggtaa
     5341 attgatggtc gactccggcg cggctcacca gatagcgcat acgctgctgg cacgtctcgg
     5401 tctgcgttat gtacagctgt cggtgattat catcggcctg attttcggtc tggcgatgtt
     5461 ctatgaagtg gcctttatca tgttagcacc gctggttatt gttattgccg ccgaagctaa
     5521 aattccgttc ctgaaactgg cgatcccggc agtagcagct gccactaccg cacattcact
     5581 gttcccaccg cagccgggtc cggtggcgct ggtgaatgct tatggcgcgg atatggggat
     5641 ggtttatatc tatagcgtac tggtgacgat cccaagtgtc atctgcgcag gtctgatcct
     5701 gccgaagttc ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag cagatcaacc
     5761 ggtagatatg aataatctgc cctctttcgg cgtttcgatt ctggtgccgc tgatcccggc
     5821 gatcattatg atctctacca ccatcgccaa tatctggctg gtaaaagata cccctgcgtg
     5881 ggaagtggtt aactttatcg gttcctcgcc gattgcaatg tttattgcga tggtggttgc
     5941 attcgtactc tttggcaccg cgcgcgggca cgatatgcag tgggtaatga acgcttttga
     6001 aagcgcggtg aaaagcattg caatggtgat tctgatcatc ggtgcaggtg gcgtgctgaa
     6061 gcagaccatc atcgacaccg gcatcggcga caccatcggc atgctgatgt cccacggcaa
     6121 tatctcgccc tacatcatgg catggctgat caccgtactg attcgtctgg cgacgggtca
     6181 gggtgtggtt tcagcgatga ccgccgccgg gattatcagt gcagcaatcc ttgatccagc
     6241 aactggtcag ctggttggcg tgaatccggc gctgctggta ctggcgacgg ctgcgggttc
     6301 caacaccctc acccacatta acgatgcctc attctggttg ttcaaaggtt actttgacct
     6361 gtcagtaaaa gacacgttga aaacctgggg tctgctggag ctggtcaact ccgtggttgg
     6421 gctgattatt gtgctgatta ttagcatggt agcgtaaaag agcacctgag cctgccattg
     6481 gcaggctctg ttcggattgt cggtaaagtg cgctttgttt atgccagatg cgacgctgac
     6541 gcgtcttatc tggcctacaa agggctaacg tgcaggtttt agcttcaagt aatattgcgt
     6601 acctgcatta gcaatgccct gtgatttctt tattgataaa caaaagtcac gccaataatc
     6661 gattgcacat tccctgcagt cacctgccct ccggtacgtg cgtaatttgc cgttaatccc
     6721 agacttaccg ccgaagtccc tactgctcct aacgataccg tgttattcgc tggaataatc
     6781 gtaccgttgc gcgtcaactg tacgccgacg ccctgtgcag gtgaaaacga cgcggtattg
     6841 gtgaaaatcg agttgcccgc atctgcggtt gtgccggaga ggtaataccc caggttttgg
     6901 cttttcgcac aataaacggt aagaggaatc ggcactgaac cagggtagtc cggcagagta
     6961 acggtgacat cacgagcaga aacatcgcag ccgccagtgg gcaccaccac atcattattg
     7021 gcgtaaatat tccacacaaa ctggaaatca tcgctgttat agttgttggt ctgtcgcaaa
     7081 ataagcacgg caattaatga gccagcttta atcgccaccc cgcccgcact gctcacaggc
     7141 gtcaaataaa gcgccaccgg ccacggctta tccgttctcg aattataaac aacccgcggc
     7201 gtttcactgg tggtcggaaa tggatagcta ctgccactat attttacggt cccggaaaaa
     7261 ttagataaca cgccgccata agccgagcct cgttgcagtg tgacataatc tgtaatggtt
     7321 tccgggtaat cgttatggca aaagatttgc gtcgaaagat ccacgaccag gttttgcccc
     7381 acattcacgg cgggcgcaag gtttacataa acattggcgc tgccaccgcc aatagggata
     7441 gcggtgccat tggcggtttt acaggcgaat gaccaggcat ttaccgacca gcccatcagc
     7501 agtacagcaa acaggttaat aactcgtttc attacaatca tctcttcggc ttcagctgta
     7561 ggtataggtg atgctaatca ctgcctgaat ggttccctga gtggctccgc catttaccgt
     7621 caatgctctg acctgtaacg ggaagtgcgc tgattgtgag gaatcatcca cctgaactgt
     7681 tttggttgcg ccactattca atgtgttgcc actgtcatcc tgtagctcta actggatgtt
     7741 ttgcgcggtc ccctggtttt tataatatcc ggtactgtcg gctgccccgc tgaagctggc
     7801 agtgaccctc gacgttccca ccggacaatt agtcaactca agcgcaacat catgccaggc
     7861 cgatgccgcc ccggcagaca tcagactgaa agaataaaga tcgccgagat ctaccgtggc
     7921 attggtggtg gaaaccgtgc acggtttggc gacgacctta ccgttcaccg tgatggtgac
     7981 atcggctgcc tgtatcgtcg cacttgcgag cgccaatatt gccgccaata cattcccacg
     8041 tttgcaccat ttcatgagca tctccagtta ctgatattca agagtgaagg tagccgtggc
     8101 attgatatgc cccgcagtga caggcacctg tgtcgccatt agccgggcgt aaaaattcag
     8161 cgtatttggt ttacccggcg tcagggtcgt ccacgaaatc gcggacgatg gggcattaag
     8221 gggtatctga ttttgttgct cattcagaag ctgtattccc agtcccgcag ccgctgacac
     8281 cgtattttca agtgcaagca ggttggcatt gtggctatct gcaacaccgg taaacccaac
     8341 ttttacggca gaaacggcat taccacaggg tgacagcaaa atacgaaatg gaacgacagg
     8401 agtcgtcgcg ccaatgttgt taaattgctt cgccgcgttt tccatcagat caacagtaaa
     8461 attggttgat tcagcggcca cactacagcc gttatctctg acatagccgc ggatagtaat
     8521 cgtgctatcc gcagcaaaag cgtgacttac cgccagccac aaaaaagcgc acagaagata
     8581 aaaaggtttg tttctcatca cgccccctta acgacattca gctgatagct gggttaataa
     8641 ctgctgctga ctctctggtg gcagttgata attggcgaca cagtgagcat tttcctcttc
     8701 tccccatttc acctgaactt ttcccgctaa aggcattccg ctgaggtaaa cctgaccatt
     8761 atccgcaaca atgccgctac tctggctact ctctgatgtc accatcgccc caaacggcag
     8821 cggcttatta ttgtgggtta gcgtcatgag cagttttatc ccaacgcgcg ctttaaactc
     8881 tgctcgcacg atcgccccac gggtgggaac aacgttagcg accgcgttat ctaaatcgac
     8941 gttatcagcc agggtattgg tatccagcgc tactctgttt tcccgatatt cagtggcata
     9001 aggcagcacg gcataaccgc gccagtcggt acgcaccccc gtctggtttt cgacttttgc
     9061 atcttttgcg ccaggcgctt taacaagcac caccgtatcg tttaacggct gccccagcgt
     9121 tacgccattg gcatgagcca gtaccccacc gctgactccg taatagagct gcttaatatc
     9181 atcgctatgg ctgtaaccga tgttggcatt gccgtaacca ccgcgataat tcagcgtggc
     9241 gtagcctgta cttccgctat taccatcacc tcccccggcg taaccggttt gcacgctata
     9301 gctgaggttg ttgtcttcca gcaacgtacc gtatacaccg gccagattgg tcatccgacc
     9361 gttgagatcg tgtgacatgc tgtaactggc actggcatgt cgccactgag atttactgtc
     9421 agaacgcagc cagtggctga aaggaatatt gacgttaagc gctaacatct gatcccgtcc
     9481 tttttgccag gcgtttttcg tcaggctata gctgagcgtc cagttgatat cttcgaatgc
     9541 agtatttaaa ccagcctgga attgctcatc gacattactc gttccccaat aagtttgatg
     9601 gctaccactc aaatacagtg ttgatgtgcg cccgagttgc tgagtaacgg tgagttgtaa
     9661 tttcccgcgt ttgttataag cgagattgta atagtaggtg aatttcggct taacctgaat
     9721 aacaccatcc tgtgtttcga tgttgtagcc attcattcga ctgtatgttg tatcagcgaa
     9781 attaaaatat ccgctggtcg aataacggta acccactaac tgaatattcg tgcctgattc
     9841 attgagcgat ttgttataga gaaaacgcac cgattgtccg tcatgctgac tgtcatcggg
     9901 aagtgtggaa ttagcctgcg tcatatccac agacagagcg cccagtgccc ccatgttttt
     9961 cccgataccg aaattaaacg cacgataacg atccgccagt tgcgttccac catatattgt
    10021 ccagccagcc ggaaggccgt ggagtaatgt actctggaaa aagcggggtt tttcctgctg
    10081 cgcatttcca ctacggtatt ctcctgccgt aatggaataa cgagtatgcc cttcacgttg
    10141 caaaagcggg actgacgaat agggtacggt aaagatctgc gtgctgccgt cagcctcttt
    10201 aatcgttacc tgcaagtcac cactattacc tgcggcatag atatcgttga tggtaaaagg
    10261 ccccggcggc accgtactat tataaatgtc atacccattt tgtttaatag tgacctgtgc
    10321 agtaccacga gcaataccgt ggatcaccgg ggcaaatcct ctttggctat cgggtaacat
    10381 attgtcatct gaggccaatt gtgcgccgcg aaagttaata ccatcgaaaa tatcgccctg
    10441 ggtataacca tcacccagcg tcagccggga acgtaacgga attatgtctc gctcaagcca
    10501 ggtattgata tgctgccatt tatttttgct acctgatgaa ctgtcgctac tgttataact
    10561 ccaggtggta ttgtcgcgta aacgccacgc accaatattt aacccactct gtaggtttaa
    10621 atatgcataa tggctgttac ccccaatccg attctgtaca ctatttccgc tgaaattata
    10681 attgagcaat ccggcattaa taccgggatc ccataactca ggaggaatat aaccacgcgc
    10741 gcgattactc ataaatgcct gagggatcgt caggttgagt cgctgctgac caacatccag
    10801 atgcgcagta gcgtcatgga tcattgaggt taatggcacg caggcatcat ccgccagcag
    10861 attcataccg gagacagaag ccgtattcag ccccatactt gcaagttgcg cgcgtgtcag
    10921 gcagggaaca atcccttgtt cactgtcgcc cgtattaaat gtgacatcac gcgttgccat
    10981 ataaccatta ttcaaataga tatcgacacg atacgtccct ggcggtaatt cttgcccatt
    11041 ttcaaaacgc gataaatcgg ccacagcctg gggatcatcc gctaaaaagc gcggattaaa
    11101 atagagttcg gcagatgaca aaggtgcctg tgcggcaaaa gcacaggcga caaagagccg
    11161 gacaaaaaaa ccagccaaac gatgcttacg aatatgcaag cattgtgtgt ttcgctggta
    11221 aagtcttaaa ttcagatatg acattattcc tccggtttaa aatcacccgg cagtcaattc
    11281 tttttattca ggcgaaaaat tccccctgcg ttattccatt acgcccgtca ttttgggggt
    11341 aagtgcgcca taatcattta ttgttcggta agtaatattg cttcctgcat cagaaggcaa
    11401 tttaaccgtg ctttcgccca ttggaggcac caatgcattt tcaagaaccc gggttccggc
    11461 attcaactct gttaccgtca ggtaataggg tgtcgggtta atcagagtca gagaattcgc
    11521 gctacgacga aatcttaatt tttctgcggc ctgatcgggt ggcaacgcta atttagccgg
    11581 gcgatagtac agtttaatgc ggctgataat tgcgagctgt agcgtattct cagtcaattt
    11641 tgatttatcc attgacggaa tcgctttaac gttcatccag aataaacttt cccgatcctg
    11701 tggcaattgg ttatttgttg catcaagaat acgtaaggta ttctcttttt ttcccttcat
    11761 cgcaaacaga ggtggcgtca cgataaaacg accatccttt acaccatcgg cattttccac
    11821 ccatgattga attaaatagg tgctattttc atcattattt gtcacggcaa gttgcacttg
    11881 tttttgccct gccggataaa ttacgcgggt cgcacctaag gccactcccg cttcagcgcg
    11941 tccggcaacc atcattgcca tgaacatcag gatacctgcc agcaagcaga atgttatttc
    12001 ctgcgatttc cttacattga cgtttttatt actcactgtc ctgttcctgt tatcacatta
    12061 tctgctgaac aattactgat aggttaaaga gaaccaggcc tgggcattgg cgatgccgcc
    12121 agtaacccga cgcccggtag cacgatattt ggcgatgaaa tgtagcgaag tagagcctga
    12181 gtaaagccgt ttccagtttg ctggaggacg attaatcggt acgaggtttc cttcatcatc
    12241 aaataacgct acgccaatgt ttgtggctat ccctggcccc tctcccacgg aaagcacatc
    12301 cggattttta ccatccgcga caccgtgaaa cgccacaccc acacgttcac tcaccaccgt
    12361 gctacattcc cgtaaatgaa taacaaaagg caccggtgcg ctatcttccc ctaccgcatg
    12421 aaaccggtta ctgctgattt gccccatatt gaccgtcatt tgtttatctc cggcttcaat
    12481 ccggcaagtt tccgcaataa tgacgccctg aaattgcata tttccgccgg gcaacgtggt
    12541 attccattta tttccggcca gagcaaacat tggcagcaac gacgctaaca gaaataatct
    12601 cttccttttc atcgttattt ttatcgcaca agggtgggca tccctgcccg taatgacgtc
    12661 cctgaacctg ggtaggttat tgatactgaa ccttgaaggt cgcatccgca ttagctgcgc
    12721 caggggttgc ctcgccgatt gcataataac gcgcctggaa cggaatggtg ttggtaccgt
    12781 tattcaaggt tgtttgtgca ctaaatgtcg caccgtccag cgtcagctca gcacccgttc
    12841 tgtccagtat ctgcacacca acatttgttg cgctacccgc agctgaactc tgcagagcca
    12901 gtactttagg atgagcactg tcaatcgcag tccccaagaa ggcaacagcg gcttttgtgg
    12961 caacactggt atcgcaatca ttcagctgaa tgttaaaacc gacagcagag ctggttgctc
    13021 cagtctgctt cagactagcg gtacgaacct ggcccaactg aacggtttga tcaacagagc
    13081 ctgcatcaac tgcgcaagcg gcgttaacaa cttccccttt aaagtgaacg gtcccaccat
    13141 ttaccgtcgt gggtgtagtt tcagccagag ccgctgtaga actcagggac agagccgaca
    13201 gaacaacaat tgccagagtt ttaattttca tactgctttc ctttcaaaaa actatttcta
    13261 aatcgacatg ggcagtcgtt ctgtacactt tgttttgtca acgagtttga ctgccaacac
    13321 tgcacagttt ccccccaaaa gatgaaacat ttggggccat tttgactcat agaggaaagc
    13381 atcgcgcaca aactttttca gtttatttgt tggcttgata ttctattgtt atctt
//
LOCUS       DAZKHC020000107        13102 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZKHC020000107 DAZKHC020000000
VERSION     DAZKHC020000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.107"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(230..718)
                     /locus_tag="ACHOSM_RS19150"
                     /old_locus_tag="pgaptmp_003830"
     CDS             complement(230..718)
                     /locus_tag="ACHOSM_RS19150"
                     /old_locus_tag="pgaptmp_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4560243.1"
                     /translation="MAQRGVNKVILIGTLGQDPEIRYIPNGGAVGRLSIATNESWRDK
                     QTGQQKEQTEWHKVVLFGKLAEIASEYLRKGSQVYIEGKLKTRKWTDDAGVERYTTEI
                     IVSQGGTMQMIGARRDDSQSSNGWGQSNQPQNHQQYSGGGKPQSNANNEPPMDFDDDI
                     PF"
     gene            888..1445
                     /locus_tag="ACHOSM_RS19155"
                     /old_locus_tag="pgaptmp_003831"
     CDS             888..1445
                     /locus_tag="ACHOSM_RS19155"
                     /old_locus_tag="pgaptmp_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4560244.1"
                     /translation="MKGKTAAGGGAICAIAVMITIVMGNGNVRTNQAGLELIGNAEGC
                     RRDPYKCPAGVWTDGIGNTHGVTPGVRKTDQQIAADWEKNILIAERCINQHFRGKDMP
                     DNAFSAMTSAAFNMGCNSLRTYYSKARGMRVETSIHKWAQKGEWVNMCNHLPDFVNSN
                     GVPLPGLKIRREEERQLCLTGLVNE"
     gene            complement(1737..2756)
                     /locus_tag="ACHOSM_RS19160"
                     /old_locus_tag="pgaptmp_003832"
     CDS             complement(1737..2756)
                     /locus_tag="ACHOSM_RS19160"
                     /old_locus_tag="pgaptmp_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head processing protein"
                     /protein_id="HGU4560245.1"
                     /translation="MTDVLKTVTDRFCLYSNARKGRQNGRQYVLSAVKTMLESKETQE
                     GLRLGELFGYYGHGRRQLTGKLEVPETSVIMVEGRPVVIDNVPACRTVAISVDDNGIV
                     THTQEILNTEPGKIVAAMIESRAGGWSWATGGRESGKIAVTTSFHGVDYVTTPNYISL
                     DHPASAGMFESADSKSLLAESLAAHGYSDESVQAVISHYSKMAELEMMVEATERTAEL
                     ETALLESQGRHLEAMAKIADAEARIALLEKTAGIRDDVLAAMQDELDNLPIFVSAAQK
                     DAFRLKKPGDAKIVATLFESLIKVGARNLPVTKKIKEVPQAANVQAPRETSIITFNNS
                     INPFK"
     gene            complement(2749..4458)
                     /locus_tag="ACHOSM_RS19165"
                     /old_locus_tag="pgaptmp_003833"
     CDS             complement(2749..4458)
                     /locus_tag="ACHOSM_RS19165"
                     /old_locus_tag="pgaptmp_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4560246.1"
                     /translation="MADNKITLSSVRKALAGVFKDNGERDNILLSALAVHGGSGYLFS
                     RAGAPVQLSGFLGGKPGDSGIAGDGLVDGSRFIFDEVQLPEDRLQRYPLLEEMAVYST
                     IATALNIHITHALSFDKKTGQTFSIVPVHNGNDSDYDAAQALCDELMNDIGRTINKEV
                     AGWAFIMSVFGVAYVRPYAKEGIGITSFECSYYTLPSFIKEFEVSGNLAGFSGDYLKD
                     ASGKMVFADPWAIIPMKIPYWRPKSNLMPVHTGHKAYSLLDNPEERTPIETQNYGTSL
                     LEYAYEPYMNLRSAIRSLKATRFNASKIDRIIGLAMNSLDPVKAADYSRTITQTLKRA
                     ADLMEKRARGANNMPTVTNTLLPIMGDGKGQMTIDTQTIQADINGIEDILTYMRQLAA
                     ALGLDYTLLGWADQMSGGLGEGGFLRTAIQAAMRASWIQQGVEEFIQRAIDIHLAFKY
                     GKVYPEGDRPYKIEFHSVNTALQQEHNDNRDSQANYATIVTQILDAVSNNSVLANSDA
                     FKRYLFSDVLEIDEKISEALVNELKAKSEDDDHLMDSIIKTPPQELAQILESVFKEGN
                     DND"
     gene            4534..11301
                     /locus_tag="ACHOSM_RS19170"
                     /old_locus_tag="pgaptmp_003834"
     CDS             4534..11301
                     /locus_tag="ACHOSM_RS19170"
                     /old_locus_tag="pgaptmp_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGU4560247.1"
                     /translation="MNKLSMGVFRCSSVSEILKYIRAITSHRAPIKYGVEKVEGKSYD
                     RLRREANQKAIDLLNSLVDGATLTDEQRQILAGYTGEGGIGGSVSEYYTPKPIAEGVW
                     EIMKLYGADVGNTLEPSAGTGVFNETKPVGTVMTATEISSVSGRINQLLHPEDSVQIS
                     PFEQLAVSTPNDSFDHVVGNVPFGGRDNTRNIDKPYAEETDMGSYFMLRMLDKIKPGG
                     FMCVIVPPSIVSGSNMKRLRLRLSRKAEFLGAHRLPTGTFDANGTSTVVDVVLMRKHP
                     AEMAEKIPLVDESTLELANVLWPTFISGKWFEKDGRRFVHGTQEKGFQGRIEVRADGQ
                     IDNQALKAKLIHRFESRIDWSLLDMAEPSPTADVVDEGEMRLINGVWQKYAGGRWIEA
                     DAGKELKIDAASYGADSWEALQRNLTTTEGRLGMTFTQMANVRDKYTTSISDDMVQLV
                     DWINSQPEKYRERLYRGAMIGRMLIEYQDMKAAGHSAEQIEQQRLSLVSRLQAEIDRF
                     GNPGRGPIAKLSGSGARAWFAFRGAIKLDGTISDELTGKLVTHDSSASYDSTSYQDTL
                     RYLYSDLTRDPIQLDDFRLAFTGELPASDEELLNLLASTPGIAVSPYGGIVPFARATS
                     GDINEIVVPKQEFLATLPDGPVKNNVLNQLAAIEEKRIKTPAENIRFKLNSRWFDRSV
                     ILEFLQENGYPDLRYVQSVQLEGDEMVSDTYHGGDGLFVGHRYGVVQRKDKETGEIRY
                     EWDRKSGENATGFPAQLEKYLNGARIGGKDSATANGYREQMALLEDQFNKWIKTHDRY
                     DELVAKYNDVFNSNIPYEHSGDPLGLKGLSGKRQPFDYQNSEVRRLSEDGRGILGFGT
                     GLGKTTTALALEAFNYENGRSTRTAYVVPKSVLENWYYEAKEFLSEAAFSNYLFVGLD
                     VLMDGDQIRQVPVLDENGKPVLGTDGTPLMRDALKLADEATITARMNAIPHSNYRAVV
                     FTKEQYARIPLRDDTVDEHAQDMLYDFVAAGRVASAMDSDSHRKEAARRRVLSEYSDT
                     GTEKAEKYPYFEDMGFDSVIADEGHNYRNSYKNGREASQLAYLPTSAVAQSARDMAIK
                     NAYLMKKNGGRGPVLLTATPVVNTPIDAYNMLSHVLPKEYWQKMGIYGPDDFVKFFGK
                     TRLETVQKISGEVEEKMALVGFENLDALRGIFHRWVTLKTAEDVKDTVEIPELDEHQQ
                     DAPLTEEQLAAYEELRQQAEAAAKANNGVTTSVNEDGVIEHEKARPIFSIIRDMDRVC
                     TDMDLYYRRITYRFLPEYADAVQQLADSLPKQATSEDDDSDDSITQQSQYSLIDKGEF
                     IQLQVPEAFEQEVNKRLARFGIDEQTVTHPVTPKYAKLIATLKEFFPEGKQIIFTDEK
                     TQHQKLKRIICNALNLEPSKVGILNAQTVAEAGKTGQKLKAVKPPKELPDEPTDAQIA
                     KYNEQMALYDAYIAQQNEMSLGGLEKIAADFQEGRTPIIICNKKAEVGINLHRGTTDI
                     HHLTLPWTPASIAQRNGRGARVGSNRASVRVHYYCGKGSFDEYRLKTLKRKAGWISDI
                     LRSDKSEMENADANDMIEMQMYTAKDDGERLAMMQVQMDKAKAAQRARQKEQATIDLQ
                     NYIKAQHAAGEDVEVLTAELERSKAELEKTTTEVAKFKQAVMAKAADNADWKARWGSV
                     HHTDRTLLAQYRASLKSAIQRKANISQAISRYEKLLNRTQKAATDIKRLRPLVEDAIN
                     KGILDVDPDLVNHASEFLVIGDRSWRVGQYYDCAGDIVRIKSLDFDSQRADVEIIFTF
                     KGTKSGNWDVKTLDKQVDVTPDEDAVMQKISGGVSIAGINDIISCDDFYRFQQRGMIK
                     ITDSYGVQTTESGYSIDFVGTYTDPLKHAVYPDRRDGALKSSIAKWVLGMMSEGNNRQ
                     IRSAETFLVELFGSNYGDVIASYGDTLSPEAIQEKIADAIARMPEKTNQGATRNGDSE
                     LEVTNAIFGTNEFRASDYEITTAQFGTIGIYSNKDEIKQAMDAASARIAAERKANLNH
                     AVAALTQSWVTAIREAATTGKITPAIADVVNDGSKFMDAYKMDAVQLPSAYGQLSYRM
                     TYNLVSMFTDLAILGLVDLNEVTPELLSMRKNHVEILQSINTVLAGRTDEEKQADADR
                     INLALGNITEEEIAARNEKQEELSSIQGDATSIAQSLGLNYRVSTADLKMMYAPKFAA
                     GEVFGLQEASGMKGVLFRAKDAIKAKFGARWLPAKAKNSDFLGNWWIIETKHNVADVL
                     AVIQQYA"
     gene            11335..11775
                     /locus_tag="ACHOSM_RS19175"
                     /old_locus_tag="pgaptmp_003835"
     CDS             11335..11775
                     /locus_tag="ACHOSM_RS19175"
                     /old_locus_tag="pgaptmp_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HGU4560248.1"
                     /translation="MAILSDTIKPNKTYLEAVLRTALLGKTEDEYVDFFLSGLRGRLL
                     KNPRLYRSYGPYWPEIKKLLLERGYGNFGRLVDRDVRKIYRYDRPALTLIAATLYSQE
                     RFDNGQIYSAWHLLPVPEEVDDQDYEFESYDLEVEALAQAGEKT"
     gene            11772..12020
                     /locus_tag="ACHOSM_RS19180"
                     /old_locus_tag="pgaptmp_003836"
     CDS             11772..12020
                     /locus_tag="ACHOSM_RS19180"
                     /old_locus_tag="pgaptmp_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="modulator protein"
                     /protein_id="HGU4560249.1"
                     /translation="MKKRYYTVKHGTLRALQEFADKHNVEVRREGGSKALRMYRPDGK
                     WRTVVDFKTNSVPQGVRDRAFEEWEQIIIDNALLLNAD"
     gene            complement(12141..12746)
                     /locus_tag="ACHOSM_RS19185"
                     /old_locus_tag="pgaptmp_003837"
     CDS             complement(12141..12746)
                     /locus_tag="ACHOSM_RS19185"
                     /old_locus_tag="pgaptmp_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4560250.1"
                     /translation="MSDTSTNEIVPHLPLIFENEANGILQDLACTPSLMGMMMFATEE
                     ELKAARSHIIDVFEECYRAGHLRIMGFTNEGQLFGYALIFGHPSGNLPLYCHKIYVYE
                     QYRGNGLGSKILAEILAFPNEVGLICQSALVPFYESAGMHFKGNYTTPPVANFTKTRG
                     MYEGLCVMSTNKTDKINDIPIFMLNNRDIDTIIQAMVSAKK"
     gene            complement(12893..>13102)
                     /locus_tag="ACHOSM_RS19190"
                     /old_locus_tag="pgaptmp_003838"
     CDS             complement(12893..>13102)
                     /locus_tag="ACHOSM_RS19190"
                     /old_locus_tag="pgaptmp_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturation control protein"
                     /protein_id="HGU4560251.1"
                     /translation="EHVLTLLCCFATEYNNVASADYIRAMAGEIDTHQEPSLLSPLIN
                     IIRGTHALTQKQVPQGLLTGKGNFF"
BASE COUNT         3344 a         3166 c         3444 g         3148 t
ORIGIN      
        1 atagagcaag tcgcctgctt aattgctgaa gaaagaaata caaatttgta acgctgtgga
       61 tcttctttat gcatccacaa tcagtaaata ttggatactt gcaaggtgcg cgaaacacta
      121 aagattgtca attaataatt aacacctctt atagttacta gaaataaaaa tcccctgtat
      181 taacaggggc ctgatagata attaattaca taaacgtctt aggtaaatcc taaaacggaa
      241 tatcgtcgtc aaagtccatt ggaggttcgt tattggcgtt gctctgaggt ttaccgccac
      301 cactgtattg ctggtggttt tgaggttggt ttgattgccc ccagccattt gaggactgtg
      361 aatcgtcacg gcgagcgccg atcatttgca tggtgccgcc ctggctgacg ataatttccg
      421 tcgtgtaacg ttctacaccg gcgtcatctg tccacttacg ggttttaagt ttcccttcga
      481 tgtatacctg agaacctttt cgtaaatact cactcgcaat ttcagcaagt tttccgaaca
      541 agacgacttt atgccattct gtttgctctt tctgttggcc cgtttgcttg tcgcgccatg
      601 attcattcgt tgcgatgctg agtcttccga ccgcgccgcc atttggtata tacctgatct
      661 ccgggtcttg ccccagggta ccaatcagga tgactttgtt tacaccgcgt tgtgccactt
      721 atcttaccta ataaaataaa tcaattagag caataatgta tatctttgaa acatagctaa
      781 caagtgattt gcattatcct gtgccttcta aagggatcga gtcagtcggt attggctgtg
      841 aatgggtgtt tgtcctggag cgtaaaaaat tcgcttatga ggtctttatg aagggaaaaa
      901 cagccgcagg aggcggtgca atttgcgcta tcgcggtgat gattaccatc gtgatgggta
      961 atggcaatgt gcgaaccaac caggcggggc ttgagcttat cggaaacgct gaaggttgcc
     1021 gacgtgatcc atacaagtgc ccggcgggtg tatggactga cgggattggt aatacacacg
     1081 gggtaacgcc tggcgtgcga aaaaccgacc agcaaatcgc cgctgattgg gaaaagaata
     1141 tcctgatcgc tgaacgctgt attaaccagc acttccgggg caaagacatg cccgataatg
     1201 ccttcagcgc aatgacaagc gcggcattca atatgggatg caatagttta cggacctact
     1261 acagcaaagc gcgaggcatg cgagtcgaaa cgtccatcca caagtgggcg cagaaagggg
     1321 aatgggtgaa tatgtgtaac catctccctg atttcgtgaa cagtaacggc gtccccctgc
     1381 ccgggttaaa gattcgccgt gaagaagaac gccagctttg cctgacgggg cttgtcaatg
     1441 aataaactcc ggcagctccg ccgactttcg acaatgaagt tatcgctggc ggcgatagtt
     1501 tttgactcga ttttcatggc ggtatatgtg ctcaatgaga cgtggccact ggaaccgcta
     1561 ttgtatgccg ggcttcggct gtgcctgact tttttgagca tggctgcaag attgatgcag
     1621 cagaaagaaa ccgcttcaga ttgtccacgc cgcgcggtgc gcaaatatat ggcacgcagg
     1681 cgaaggcgat aatagttaac gagaaccccg gcagccgccg gggttatttt tggtggttat
     1741 ttaaacgggt tgattgaatt attaaacgtg atgatgcttg tctcacgcgg tgcctggacg
     1801 ttagccgctt gcggaacctc cttaattttc ttggtgacag gcaagttgcg tgcgccaact
     1861 ttgatcagag attcgaaaag cgtggctacg atttttgcat caccaggttt tttgaggcgg
     1921 aatgcgtctt tttgggcggc ggagacgaaa atcgggaggt tatccagttc gtcttgcatc
     1981 gctgccagca catcgtcgcg gatacccgct gttttctcca acaaagcgat tcgcgcttca
     2041 gcatcagcga tcttggccat tgcttcaagg tggcggccct ggctttcgag tagtgcggtt
     2101 tccagttcag ccgtacgctc tgtcgcctcc accatcattt ccagttcagc cattttactg
     2161 taatgagata taacggcctg cactgactcg tcagagtatc catgcgcagc cagggactct
     2221 gccagtagag atttagaatc cgcgctttca aacattccgg cgctggcagg atgatccaga
     2281 ctgatatagt tcggcgtcgt cacataatcc acaccatgga agctggtggt tacagcgatt
     2341 ttcccggact cgcgcccgcc agtggcccag ctccagccac cagcacggct ttcgatcatc
     2401 gcggcgacaa ttttacccgg ctctgtgtta agaatttcct gtgtatgggt aacgatgccg
     2461 ttgtcgtcaa cggatatagc cactgtgcgg caagctggaa cattgtcgat aacgaccggg
     2521 cgaccttcca ccatgatcac gctggtttca ggcacctcca gtttaccggt tagctggcgg
     2581 cgaccgtgac cgtaatagcc gaaaagctct ccaaggcgta aaccttcctg agtttccttg
     2641 ctttcaagca tggtcttgac cgcgcttaat acatactgtc gcccgttctg gcgacctttt
     2701 cgagcattgc tatagagaca aaagcggtca gtgaccgttt tcaaaacatc agtcattatc
     2761 gtttccctct ttaaagaccg attcaaggat ttgcgccagt tcctgtggcg gtgttttgat
     2821 gatggaatcc atcaggtgat cgtcgtcctc gcttttcgct ttcagttcgt taactagtgc
     2881 ttcagagatt ttttcgtcaa tctccagcac atcgctgaac aggtaacgtt tgaatgcatc
     2941 ggaattggcg aggacgctgt tattgctgac ggcatcgagg atttgcgtaa cgatggtggc
     3001 gtagttcgcc tgcgagtcgc ggttatcgtt gtgctcttgt tgcagagcgg tattaacgga
     3061 gtggaattcg attttgtacg ggcgatcacc ttccgggtat accttgccgt acttgaaagc
     3121 aagatgaata tcgatagccc gctgaatgaa ctcttctacg ccctgctgga tccatgaggc
     3181 gcgcatggcg gcctgaattg ccgtgcgcag gaatccacct tcaccaagcc cgccggacat
     3241 ttgatctgcc caccccagga gggtgtaatc gaggccaagt gctgccgcca gttggcgcat
     3301 ataggtgaga atgtcttcaa tgccgttgat gtcagcctgg atggtctggg tatcaatagt
     3361 catctgtccc ttgccgtcgc ccataatagg cagcagggta ttggtcaccg taggcatgtt
     3421 attcgcgccg cgtgcgcgct tttccatcag gtcagctgct cgtttaagcg tctgagtaat
     3481 ggtgcgtgaa taatcggctg cttttaccgg atccagacta ttcatcgcca gaccgatgat
     3541 tcggtcaatt ttcgacgcat taaaacgcgt tgccttcagc gagcggatcg ccgaacgcag
     3601 attcatgtac ggctcgtagg cgtattcgag caagctggtc ccgtaattct gggtttcaat
     3661 cggcgtgcgc tcttccggat tatccagcaa gctgtaagcc ttatggccag tgtgcacagg
     3721 cataaggttt gacttaggcc gccagtaggg gattttcata gggataatgg cccacggatc
     3781 ggcgaaaacc atttttcctg acgcgtcctt cagataatcg ccgctaaatc ccgccaggtt
     3841 gccgctgacc tcgaactcct tgatgaagct cggaagggtg taataggagc actcaaaaga
     3901 cgtgatccct atgccttctt tggcgtatgg cctgacataa gccaccccaa atacagacat
     3961 gataaatgcc cacccggcga cctctttgtt gatggttcgc ccgatgtcgt tcatcagctc
     4021 gtcacacaac gcctgcgcgg cgtcatagtc actatcgttt ccgttatgta ccggcacgat
     4081 agagaaggtt tgtccggtct tcttatcgaa agagagcgcg tgcgtaatat ggatgttcag
     4141 cgcggtggcg atcgtgctgt aaaccgccat ctcttcgagt agcggatagc gttgcaagcg
     4201 gtcttccggc agttgaactt catcaaagat aaagcgactt ccgtccacca gcccatcgcc
     4261 agctatgcca ctatcgcccg gtttgccgcc taagaagccg gacagttgta ccggtgcccc
     4321 tgcgcgagaa aacaaatacc cacttccgcc gtgcacagcc agcgcggaca ggaggatgtt
     4381 gtcccgttct ccgttgtctt taaaaacccc cgccagcgcc ttcctgaccg aggatagcgt
     4441 gattttattg tctgccaaga ttgcacctta attagaataa ttcgcatcgt gtttgaacgg
     4501 aatttaacac tagtcacttg ttaaggatta ccaatgaaca agctatctat gggggtgttt
     4561 cgctgttcaa gtgtcagcga aatattgaaa tacattaggg caataacatc tcaccgagcg
     4621 ccgattaaat acggcgtgga aaaggtggaa ggcaaaagct atgaccgact gcgccgggag
     4681 gcgaatcaga aggcgataga tttgcttaat tcgctggtgg acggcgcgac actgacagat
     4741 gaacagcgcc agatcctggc tgggtacacc ggtgaaggcg gcattggcgg gtccgtctcc
     4801 gaatattaca caccaaagcc tatcgctgaa ggtgtctggg agatcatgaa gctctatggc
     4861 gcggacgtag gtaacactct ggaaccatcg gcgggcaccg gcgtttttaa tgagacaaaa
     4921 ccggttggta cggtgatgac cgcaactgag atcagcagtg tttccggtcg tataaaccag
     4981 ctgttacacc cggaagacag cgtacagatt tccccgttcg aacagctggc tgtaagcacg
     5041 cctaacgatt cattcgacca tgttgtgggt aacgttccgt tcggtggtcg tgataacaca
     5101 cgcaacatcg ataagcctta cgcagaagaa acggacatgg gttcttactt catgctccgc
     5161 atgctggaca agataaagcc aggcggattc atgtgcgtga ttgtgccgcc gtccattgtt
     5221 tcaggttcaa acatgaagcg gttacgcctg cgcctatcac ggaaagctga atttcttggt
     5281 gcccaccgct tgcctaccgg tacttttgac gcaaacggga ccagtacggt cgtagatgtg
     5341 gtgctgatgc gcaaacatcc ggcagagatg gctgagaaaa tccccctggt ggatgaaagc
     5401 actcttgaat tggcaaatgt gctttggcca acgtttattt ctggcaagtg gtttgaaaag
     5461 gacggccgcc ggtttgttca tggtacccag gaaaagggct tccaggggcg tattgaggtt
     5521 cgtgccgacg ggcagattga taaccaggct cttaaagcga agctgattca tcgtttcgaa
     5581 agtcgtatcg actggtcttt gctagatatg gctgaaccgt caccgacagc agacgttgtt
     5641 gatgaagggg aaatgcgcct gattaatggc gtatggcaaa aatatgctgg tggtcgctgg
     5701 attgaagctg atgctggtaa ggagcttaag attgatgctg ccagttatgg cgcggatagc
     5761 tgggaggctc ttcagcgtaa cctgactaca acagaaggcc gtctcggtat gacatttacc
     5821 cagatggcaa atgtccgcga taagtacacc acatcaatca gcgacgatat ggtgcagctg
     5881 gtggactgga ttaacagcca gcctgaaaaa taccgtgaac gcttgtatcg cggggcaatg
     5941 attggccgga tgttaattga atatcaggat atgaaggccg ccgggcatag tgctgaacaa
     6001 atcgaacagc agcgcctttc tctggtatcc cgtttgcagg cagagattga ccgttttggt
     6061 aaccccggtc gcggtccgat agctaaatta tcgggaagcg gtgcgcgcgc ctggtttgct
     6121 ttccgtggtg caattaagct ggatggcact atttctgacg agctgacagg aaagctggtt
     6181 acgcatgatt ccagcgcaag ttatgactcc accagctatc aggacacctt gcgttatctc
     6241 tatagcgatc ttacccgcga tccaatccag ctcgatgatt tccgccttgc gtttaccggc
     6301 gaactgccag ccagtgatga agagttgctt aatttattgg ccagcacccc aggtattgcg
     6361 gtttcgccgt atggcgggat tgttccgttc gcccgcgcca ccagcggcga cattaacgag
     6421 atagtggttc caaaacagga attcctcgcc acgctccccg acggtccagt aaagaacaac
     6481 gtccttaatc agctggcagc gatcgaagag aagcgcatca agacgccagc agagaatatc
     6541 cgctttaagc tcaatagccg ttggttcgac cgttccgtca ttctggagtt tttgcaggaa
     6601 aacggctatc cggatctgcg ctatgtgcag tcagtgcagc tggaaggcga cgaaatggtt
     6661 tctgacacct atcacggtgg tgatggtctg ttcgtcggac accgatacgg tgtcgtccag
     6721 cgcaaggata aagaaacagg cgagatccgc tacgagtggg accgtaaatc aggtgaaaac
     6781 gcgaccgggt tcccggcaca gctggaaaag tatctcaatg gtgcgcgtat cggtggcaaa
     6841 gatagcgcga cggcgaacgg ctaccgcgag cagatggcac tgcttgagga ccagttcaat
     6901 aagtggatca agacacacga tcgctacgat gagctggttg ccaaatacaa cgatgttttc
     6961 aatagcaata ttccgtatga acactctggc gatccgcttg ggttgaaggg attaagcggt
     7021 aagcgccagc catttgatta ccagaatagc gaggtgcgac gactgtctga agatgggcgc
     7081 ggcatcctgg gcttcggcac cgggctgggt aaaacgacga ccgcgctggc gcttgaggcg
     7141 ttcaactatg agaacggtcg ctccacccgt actgcttatg tagtgcctaa atcagtgctg
     7201 gaaaactggt attacgaagc aaaagagttc ctgagtgaag cggcattcag taactacctg
     7261 ttcgtcggtc ttgatgtgct gatggatggc gatcagattc gccaggtgcc ggtgctcgat
     7321 gagaacggta aacctgtcct tggtactgat ggcactccac ttatgcgtga tgctcttaaa
     7381 ctggcagatg aagccactat caccgcgcga atgaacgcga tcccgcactc aaattaccgt
     7441 gcagtcgtgt ttaccaaaga acaatacgcc cgcattccgc tacgtgatga caccgtagat
     7501 gagcatgcac aggatatgct ttatgacttc gttgccgccg ggcgcgtagc cagcgcaatg
     7561 gactccgact cccaccgcaa agaggccgcg cgtcgccggg tattgtcgga gtattcagat
     7621 accggcaccg aaaaagcaga gaagtatccg tactttgagg atatgggctt cgatagtgtg
     7681 atcgccgacg aaggtcacaa ctaccgcaat agctataaaa atggtcgcga agcgtcacaa
     7741 ctggcctatc tgcccaccag cgcggtggcg caatcggcgc gggatatggc aattaaaaac
     7801 gcgtacctga tgaaaaagaa tggcgggcgc gggccggttc tcctgactgc aacgccagtc
     7861 gttaacaccc cgatcgatgc atacaacatg ctttctcatg ttctgccgaa ggaatactgg
     7921 cagaagatgg ggatctacgg tcctgatgac ttcgttaaat tcttcggcaa gaccaggctg
     7981 gaaacggtac agaaaatcag cggtgaagtt gaagaaaaaa tggcgctggt gggctttgaa
     8041 aaccttgatg cgctgcgcgg tatattccat cgctgggtaa cgcttaaaac ggcggaagac
     8101 gttaaggata ccgtggagat cccggagctg gacgaacacc agcaggatgc accacttact
     8161 gaagaacaac tggcggcgta tgaagaattg cgtcagcagg cggaagcggc ggccaaagcc
     8221 aacaatggcg taacgacctc ggtcaatgaa gacggcgtga ttgagcacga gaaagcccgt
     8281 ccgatcttct caataatcag ggatatggac cgcgtatgta ctgacatgga cctgtactat
     8341 cgtcggatca cctatcgttt cctgccggag tacgccgatg cggtgcagca gctggcggac
     8401 agtttgccta aacaagccac cagcgaagac gacgacagtg atgattcaat cacgcagcaa
     8461 tcgcaatact ccctgataga taagggcgag tttattcagt tgcaggttcc ggaagcgttc
     8521 gagcaggaag tgaataagcg cctggccagg tttggcattg acgaacagac cgtaactcac
     8581 cccgttacgc ccaaatacgc gaagctgatt gccacgctga aggagttttt cccggaaggt
     8641 aagcaaatca tcttcaccga tgaaaaaacg cagcaccaga agctcaagcg cattatctgc
     8701 aatgctctta accttgaacc ttcaaaggtg gggatcctga atgctcagac ggttgccgag
     8761 gcaggtaaaa ccggtcagaa actgaaagct gttaaaccgc cgaaagagct accggatgaa
     8821 ccaacagatg cgcagatagc gaaatacaac gagcaaatgg ctctgtatga cgcctatatc
     8881 gcgcagcaaa atgaaatgtc gctgggcggg ctggaaaaga ttgctgccga cttccaggag
     8941 ggccggactc cgatcatcat ctgcaacaaa aaggccgagg tcggtatcaa cctgcatcga
     9001 ggaacgactg acatccatca tctgacgttg ccatggacac cagccagtat cgcacagcgt
     9061 aacggtcgcg gtgcccgagt tggctccaac cgtgcaagcg ttcgcgttca ctactactgc
     9121 ggcaagggtt ctttcgatga ataccgactg aagacgctga agcgtaaagc aggctggatc
     9181 tccgatatcc tccgttcaga taagtcagaa atggagaacg ccgacgccaa tgatatgatc
     9241 gaaatgcaga tgtataccgc gaaggatgac ggtgaacgtc tggcaatgat gcaggttcaa
     9301 atggataagg cgaaagccgc gcaacgcgct cgccagaaag aacaggctac tatcgacctt
     9361 cagaactaca tcaaggcgca gcacgcagct ggtgaggatg tggaggtgct taccgctgaa
     9421 ttagagcgaa gcaaagcgga acttgaaaag accaccaccg aggtcgccaa attcaaacag
     9481 gcggtaatgg ccaaagcagc tgataacgca gactggaaag cccgctgggg tagcgtccat
     9541 cacacagacc gtacgttgtt agcacagtat cgcgcgtcgt tgaaaagcgc cattcagcgc
     9601 aaggctaata tctctcaagc catctcccgc tatgagaaat tattgaaccg tactcagaag
     9661 gccgcgacgg atatcaaacg cctgcgcccg ctggtggagg atgcaataaa taaaggcatt
     9721 ctggatgttg atcctgactt ggttaaccat gcgagtgagt tccttgttat cggcgatcgc
     9781 tcatggcgtg taggccaata ctatgattgt gccggtgata tcgtccgcat taagtctctg
     9841 gacttcgaca gccagcgcgc agacgtggag attatcttta ccttcaaagg taccaaatcg
     9901 ggtaactggg atgtgaagac gctggataaa caggttgatg taactcccga tgaagatgct
     9961 gttatgcaga aaatcagtgg tggcgtctcc atcgccggga ttaacgacat catttcctgt
    10021 gacgatttct accgtttcca gcagcgcggc atgatcaaaa tcactgactc atacggcgtt
    10081 cagactacag agtcaggcta tagcattgat tttgttggta cctatacgga cccactgaag
    10141 catgcggttt acccggatcg ccgtgacggc gcgctgaagt cgtcaattgc aaaatgggtg
    10201 cttggtatga tgtcggaagg gaataaccgc cagatccgtt cggcagaaac attcctggtt
    10261 gaactgtttg gctccaatta tggcgatgta atcgcgtcat acggagatac gctatcccct
    10321 gaagcaattc aggagaaaat agcggatgcg atcgccagaa tgccggaaaa aacaaaccag
    10381 ggggctactc gtaacgggga ttctgaactt gaagtcacca atgccatttt cggtaccaat
    10441 gagttccggg cgtcagatta tgagatcacc acagcacagt ttggcaccat tggcatttac
    10501 agcaataaag acgagatcaa gcaggcaatg gacgcagcaa gtgcgcgcat tgcagcagaa
    10561 cggaaagcca atctgaatca tgcagtcgcc gcgctgactc aatcgtgggt aacagcaatc
    10621 agggaggccg ccaccacagg gaaaatcaca ccggcaattg cggatgtcgt aaacgacggc
    10681 tctaaattta tggatgccta taaaatggat gcggtgcagt tgccatcagc ctatgggcaa
    10741 ctcagctatc gcatgaccta caacctggta tcgatgttta ccgaccttgc catccttggg
    10801 ctggtggacc ttaacgaggt tacgccggaa ttgctcagca tgcgcaagaa tcatgtggag
    10861 atattgcaga gtattaacac ggttcttgcc gggcgcaccg atgaagagaa acaggccgac
    10921 gctgatcgga taaacctggc ccttggcaac atcacggagg aagaaattgc cgccagaaac
    10981 gagaaacaag aagagttatc atcaatacag ggtgatgcca ccagcatagc tcagtctctt
    11041 ggtctgaatt atcgcgtatc caccgccgac ctgaagatga tgtacgcacc aaaattcgcc
    11101 gctggcgagg tatttgggct tcaggaagcc tcaggcatga aaggcgttct tttccgtgcg
    11161 aaagacgcaa tcaaggcgaa attcggcgct cgctggctgc cagcgaaggc gaagaacagc
    11221 gatttcctgg gtaactggtg gattatcgag acaaaacaca acgtggcgga cgttctggcc
    11281 gtcatccaac aatacgcata acaggagcgc ccggttcgcc gggcgtcgca taatatggcc
    11341 atactatctg atacaataaa accgaataaa acatatcttg aggcggtact gcgtacggcg
    11401 ttgttaggaa agacagaaga cgaatacgtt gatttcttcc tgtcagggct acgcgggcga
    11461 ttactgaaaa atccccgcct gtaccgcagc tatggtccat actggccgga aattaaaaaa
    11521 ttattactgg agcgcggtta tggtaatttc ggtcgtctcg ttgaccgtga cgttcgcaaa
    11581 atttaccgtt atgaccgccc ggcgctgaca ctcatagccg cgacgctcta cagccaggag
    11641 cgttttgata atggtcagat atactcagcc tggcatttac tgccagtgcc tgaagaagtt
    11701 gacgatcagg actatgagtt tgagtcttac gatttggaag ttgaagcctt ggcacaggct
    11761 ggagagaaaa cttgaaaaag cgatactaca cagtaaagca cgggacgcta cgagcattac
    11821 aagagtttgc tgacaagcat aacgttgagg tgcgcaggga agggggaagt aaagctctgc
    11881 gcatgtaccg tccggacggg aaatggcgga cggtcgtcga tttcaaaaca aacagtgtcc
    11941 cccagggcgt ccgcgaccgg gcattcgaag aatgggagca gatcatcata gataatgcgt
    12001 tgcttctgaa tgctgattaa actttccgag tttatgcccg gcactcgacc gggctgaaaa
    12061 agttttatgc agaagtaatc atgtttaaca tttggagatt aaaccatttg tgatcttttc
    12121 agcattttgg agctgtaggg ttattttttt gccgatacca tagcttgtat aatggtatca
    12181 atatcgcgat tatttaacat gaagatgggg atatcattaa tcttgtccgt cttattggta
    12241 ctcatcacgc aaagcccctc atacatccct cgggtttttg tgaaattagc gactggtgga
    12301 gtagtgtaat tccctttaaa gtgcatcccc gctgattcat aaaaaggaac taaagcagac
    12361 tggcaaatca atccgacttc atttggaaat gcaagtattt ctgccagtat cttacttcca
    12421 agtccattac cacgatattg ctcatataca taaattttat gacagtacaa tggtaagttt
    12481 cctgatggat gcccaaaaat taaggcgtac ccgaacaact gtccttcatt agtaaagccc
    12541 ataatgcgta aatgtccggc tcggtaacac tcttcaaata cgtctattat gtggcttcgt
    12601 gccgctttca gttcctcttc tgtcgcaaac atcatcatcc ccatcagcga tggggtacag
    12661 gccagatcct gaagaatccc attagcttca ttttcgaaga ttaatggtag gtgtggtaca
    12721 atttcgtttg tcgaagtgtc agacatagaa aaactccttc gttttttgag ctgatgttat
    12781 cagcgatagc gatgaaggag aaacaatttt gactctctta aagaggttgc ccctcttgat
    12841 agaaaacgga gaaatggtaa acaacgatct ccgtttttaa acaaatagtc ggttaaaaaa
    12901 aatttccttt tcctgttaac aatccttgcg gaacttgctt ttgtgtcagc gcatgggtac
    12961 cgcgaattat attaatcagg gggcttagta acgatggttc ctgatgggta tcaatttccc
    13021 cagccattgc ccttatgtag tctgcgctgg caacgttgtt gtattccgtc gcaaagcaac
    13081 atagtaacgt cagaacatgc tc
//
LOCUS       DAZKHC020000108        13076 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZKHC020000108 DAZKHC020000000
VERSION     DAZKHC020000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.108"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..540
                     /locus_tag="ACHOSM_RS19195"
                     /old_locus_tag="pgaptmp_003839"
     CDS             <1..540
                     /locus_tag="ACHOSM_RS19195"
                     /old_locus_tag="pgaptmp_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGU4560252.1"
                     /translation="AYMAEVNLYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNLN
                     KAILQNINLIDSKLFCTWLTNTFLEMVICTDSNMANVNFNNANLSNCHFNCSILTKAC
                     MFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTE
                     DNINAVAMKITADIMQHAD"
     gene            complement(694..2343)
                     /locus_tag="ACHOSM_RS19200"
                     /old_locus_tag="pgaptmp_003840"
     CDS             complement(694..2343)
                     /locus_tag="ACHOSM_RS19200"
                     /old_locus_tag="pgaptmp_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HGU4560253.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2495..3844)
                     /gene="ghxP"
                     /locus_tag="ACHOSM_RS19205"
                     /old_locus_tag="pgaptmp_003841"
                     /gene_synonym="yjcD"
     CDS             complement(2495..3844)
                     /gene="ghxP"
                     /locus_tag="ACHOSM_RS19205"
                     /old_locus_tag="pgaptmp_003841"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HGU4560254.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4390..4854)
                     /gene="soxR"
                     /locus_tag="ACHOSM_RS19210"
                     /old_locus_tag="pgaptmp_003842"
     CDS             complement(4390..4854)
                     /gene="soxR"
                     /locus_tag="ACHOSM_RS19210"
                     /old_locus_tag="pgaptmp_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HGU4560255.1"
                     /translation="MEKKLPRIKALLTSGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEYEQN"
     gene            4940..5263
                     /gene="soxS"
                     /locus_tag="ACHOSM_RS19215"
                     /old_locus_tag="pgaptmp_003843"
     CDS             4940..5263
                     /gene="soxS"
                     /locus_tag="ACHOSM_RS19215"
                     /old_locus_tag="pgaptmp_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HGU4560256.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5266..6852)
                     /gene="pdeC"
                     /locus_tag="ACHOSM_RS19220"
                     /old_locus_tag="pgaptmp_003844"
     CDS             complement(5266..6852)
                     /gene="pdeC"
                     /locus_tag="ACHOSM_RS19220"
                     /old_locus_tag="pgaptmp_003844"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HGU4560257.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFLSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7278..7559
                     /locus_tag="ACHOSM_RS19225"
                     /old_locus_tag="pgaptmp_003845"
     CDS             7278..7559
                     /locus_tag="ACHOSM_RS19225"
                     /old_locus_tag="pgaptmp_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HGU4560258.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(7658..8194)
                     /gene="ssb1"
                     /locus_tag="ACHOSM_RS19230"
                     /old_locus_tag="pgaptmp_003846"
     CDS             complement(7658..8194)
                     /gene="ssb1"
                     /locus_tag="ACHOSM_RS19230"
                     /old_locus_tag="pgaptmp_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HGU4560259.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8449..11271
                     /gene="uvrA"
                     /locus_tag="ACHOSM_RS19235"
                     /old_locus_tag="pgaptmp_003847"
     CDS             8449..11271
                     /gene="uvrA"
                     /locus_tag="ACHOSM_RS19235"
                     /old_locus_tag="pgaptmp_003847"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGU4560260.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11306..11662)
                     /locus_tag="ACHOSM_RS19240"
                     /old_locus_tag="pgaptmp_003848"
     CDS             complement(11306..11662)
                     /locus_tag="ACHOSM_RS19240"
                     /old_locus_tag="pgaptmp_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU4560261.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11666..12082)
                     /locus_tag="ACHOSM_RS19245"
                     /old_locus_tag="pgaptmp_003849"
     CDS             complement(11666..12082)
                     /locus_tag="ACHOSM_RS19245"
                     /old_locus_tag="pgaptmp_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HGU4560262.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12193..12906)
                     /gene="aphA"
                     /locus_tag="ACHOSM_RS19250"
                     /old_locus_tag="pgaptmp_003850"
     CDS             complement(12193..12906)
                     /gene="aphA"
                     /locus_tag="ACHOSM_RS19250"
                     /old_locus_tag="pgaptmp_003850"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HGU4560263.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3385 a         3425 c         3198 g         3068 t
ORIGIN      
        1 gcgtacatgg ctgaggtaaa tctttataaa gtaatagcgc catatgttaa tttatttaaa
       61 gccgatctta gtttctctaa actcgattta attaactttg aacatgctga tctgtctcgc
      121 gtcaatctga acaaagcaat cctccagaat ataaacttaa ttgatagcaa actcttttgt
      181 acgtggctaa caaatacatt cctcgaaatg gttatatgta ccgactctaa tatggctaat
      241 gttaatttta ataatgccaa tttaagcaac tgccatttca actgttctat tttaacaaaa
      301 gcctgtatgt ttaatacccg tctctatcgg gttaattttg atgaggccag cgtccaggga
      361 atgggcattt ccatactccg tggggaggaa aatatcccca ttgatagtga taccctggta
      421 acactacaga aattctttga agaagattgt acctctcata ctggcatgtc acaaactgag
      481 gataatatta atgcagtcgc tatgaagatt actgcagata ttatgcaaca cgcagattga
      541 ggttaagtcg aagaacagcc aatgaattga ctttacaaga gagataactt atttgtttta
      601 gaatcatgaa tgttagcccg agctaacctg ttaaaggtgc aaaagattgc acctttttca
      661 gattacctcg ggcctggtta cgtcgctttc ggcttactga ttttcctcaa ttagcaacgc
      721 ttcaagcaaa tcgagatcgt gcagtaattt ttgcagcgtt tcgttgctga tctcccgcgt
      781 ggcgcgcagg tggtacagtt cagcacgttc agaacgcaat gccgccagac ggaagcgacg
      841 ctcaaggttc tcttcctgca cggaactttc aacgtcgtta cgtccatcgg cgcgacgacg
      901 caggttacca atgacgcgag aactgacctc cgtaagcagc tggttatcga tattctcttc
      961 ggtatccgcc gccagacgct cctccatttt ctggatcgcc acaatcgcca cttctgccgt
     1021 tgccgctcgc gcaatacgtt cttctttcaa ttgttgcgaa tggtccgcga cttcaatgtg
     1081 ttgtagcaga ataggcaaca tcaccacgcc gacaaacagc gagaagagaa tgacgccagc
     1141 cgccaggaac accagctcat agcgcgccgg gaagacgtta ccatccggca agagcagcgg
     1201 gatagagagc acaccggcca gagtgatcgc cccacgcacc ccggcgaaag acgcgatcag
     1261 gatttctcgt gtggtccacg aaccaaactc catcggcttc ttcttcagga agcggttgct
     1321 gaactttttc atcgtccaca gccagccgaa acggaccagc atcagcgccg catatatcag
     1381 aataatatcg gtaaacagca tccagatttc gacgttaggg tcgatttctg ccgccatcag
     1441 cgacgtctcc agaatacccg gcagctgcag acctaacagc aggaacacca tgccgttaaa
     1501 gacaaattcc agcatcgccc aggtgctgtt tgcgcgcagg cgcattgcca gcggcgcacg
     1561 gcgcatcaca ccggagcggg tgatggtcat cccggcggca accgcagcga ggatgccgga
     1621 aacgccaata tgttcggcaa tcagataaga agcgaatggc agcaagaaca gcagaacgat
     1681 ctgcgtcgcg ggttcatcac cgccccagcg gctgaggaat cgcagcgagc gaccatacag
     1741 ccagctcacc acaaaaccgg caagaatacc accaatggct actttcataa attcgaccgt
     1801 cgcaccgccg acggtgaaga tcatcgtccc catcgccact gccacggcaa acttcaacga
     1861 caccaggccg gaggcgtcat tcatcaacgc ttcgccctgc aaaatgccca tgattttttt
     1921 cgggatgcgc ccttcgccaa caatcccgga gagcgccaca gcatcggtcg gagaaagtac
     1981 cgccgccagc gcaaaggcgg ggatcagcgg aatgcccggc accacccagt aaataaggaa
     2041 gccaatgccg accacggtga ccaccaccag cgccagcgcg aggccgaaaa tctctcgacc
     2101 atgttcgaga aattcacggg tcggcgtttt ccagccatca gcgaacagca acggcgggat
     2161 aaacaagact aaaaagagtt caggatcaaa ctccacatgc aaaccaaacg tcggccacgc
     2221 cagtagcgca ccgatggcga tttgcataag cggaagcggg atctgaaagg gcatgacacg
     2281 agtgaccacc ccggacagcg agaccacgag ggtcattatc agtatggtga agaagatttc
     2341 catgcgttcc ctgtttgcga tgttttttta tgatctgaga aagacgttat gcctgaattc
     2401 tcattttttc agaatatcgc aaaagctcac acaatgtgcg gagaaaataa aaacgggcag
     2461 ccattgccac ccgttttcag aatccgatga aagattagat agcccagcca cccgcataga
     2521 aggtcaccag cgccacggcg ataacgaccg taccgatgtt caacttgcgc cattcgccgg
     2581 aaaccagacg accaatcacc agagtcgcga agccgatcat gatgcctgtt acgatgttac
     2641 aggtcagcac gatgaatacc gccgtaacca gacccgccat cgcatcaaca aaatcagcaa
     2701 agtcgatttt cgccacgttg ctcagcatca gcaggccaac gtacatcagc gccggagccg
     2761 ttgcataccc cggaacgagg taagagagcg gagagaggaa cagaatcagg aggaacagca
     2821 cgccaacggt gatagccgtc aaaccggttt taccgcccgc cgccgtaccc gccgcagact
     2881 cgatgtatac cgctgccgga gcagcaccca ccaggccaga gaaaacgctg ctcatggagt
     2941 cagtggtcag tgctttccca ccgtcgatga tctgcccatc tttatccagc aggttcgcct
     3001 ggccggcgac ggcacggata gttccggtgg catcaaatac tgccgtcatc accagcgcca
     3061 gaacgcttgg caggactaca ggattcagcg cgcccataat gtccaggctg ccaatcagtg
     3121 aattgccgtt ttcatcgctc aatgaaggca tggcgaaaac gccggagaaa tggacgttag
     3181 gatcgaagat caaaccgaca attgagatac cgataatggt cagcagaatg ccaccaggga
     3241 ctttcagttt ttccaggccg atgatcaccg ccagacctac cagtgacata atcaccggga
     3301 aggtcgcgaa atcacccagc gcaacgggca gaccatcaag cgggttttta atcaccagac
     3361 cgacaccatt agcggcaatg agcagcagga acagaccgat accgatcccc gtgccgtgcg
     3421 ccacaccgtg cggcaagttg cgcaaaatcc agctacggat acccgtggca gaaattaccg
     3481 taaacagcac ccccatcagg aacacggcac ccagcgcgac aggtacgcta atatgttgcc
     3541 ccagcaccag gctgaatgcg gtaaacgccg tcagggagat ggcgcaacca atcgccaacg
     3601 gcagattagc ccacagaccc atcacgatag aaccgagtcc ggcaaccaga caggttgcaa
     3661 cgaaaactgc cgcaggcggg aagcctgctt tacccaacat acctggaacg acgatgaccg
     3721 agtagaccat cgccagaaac gttgttaacc cggcaaccac ttcctgacgg acagtgcttc
     3781 cacgttgtga aattttaaac caggcgtcga gtgaaccgcc ggtacgcgct gatggcgtag
     3841 acataagaaa acatcccctg agagtttaat tttcgtcaag atgcgtggcg gacaatcccc
     3901 tgccagcgaa acgttatctt cctgctccag gtttgcgaca atttactgcg tcgcaaaaaa
     3961 gcaaacgttt agctccgcgc atacaaaacg ggtggtaaaa aaagcaaacg attatccggc
     4021 gatcatagcg gctttttcaa ccgaagtttg ctgctttttt ctcttttatt gtcttgacga
     4081 tgaaaaacca ggcaattgat gcgcaaacgt tatcgtcggt gcgcaagatt gcagcgttat
     4141 cgagccgtat accgttcagg attgcgataa aaaatcgtct gagatgacaa aaatattgcg
     4201 gataaaaagt atacttttac actggcgaag aaatgccggt gacatacaat taaagcatca
     4261 acaccaaccg gaacctccac cacgtgctcg aatgaggtgt gttgacgtcg ggggaaaccc
     4321 tcctgtgtac cagcgggata gagagaaaga caaagaccgg aaaacaaact aaagcgccct
     4381 tgtggcgctt tagttttgtt catattccag caagcgtgcg ccggtacctt cttctcctaa
     4441 gcggtcgccc gggttacgca acgggcaatc actgcgcgaa aggcagccac aaccaataca
     4501 tccgtccagt tcgtcacgca gcgccactaa ggtatgaatg cgccgatcca actcttctcg
     4561 ccattgggac gaaagctgtt tccactcttt cgcacttaac gtatgccctt cgggcaacac
     4621 gccaaacgct tcaccaatgg tcgccagcgg aatgccaata cgctgagcaa ttttgataat
     4681 tgcaacatat cgcaacacat cacgtttata tcgccgctga ttgccgctgt tacggatact
     4741 ggtaatcaac cctttacttt catagaaatg cagcgccgat accgccacac cgctgcgttt
     4801 cgccacttcg ccggaggtta gcagcgcttt aatgcggggt aatttctttt ccataaatcg
     4861 ctttacctca agttaacttg aggaattata ctccccaaca gatgaattaa cgaactgaac
     4921 actgaaaaga ggcagattta tgtcccatca gaaaattatt caggatctta tcgcatggat
     4981 tgacgagcat attgaccagc cgcttaacat tgatgtagtc gcaaaaaaat caggctattc
     5041 aaagtggtac ttgcaacgaa tgttccgcac ggtgacgcat cagacgcttg gcgattacat
     5101 tcgccaacgt cgcctgttac tggccgccgt tgagttgcgc accaccgagc gtccgatttt
     5161 tgatatcgca atggacctgg gttatgtctc gcagcagacc ttctcccgcg ttttccgtcg
     5221 gcagtttgat cgcactccca gcgattatcg ccaccgcctg tagttttatt gcccgcgcgt
     5281 taactcccgc gcgggtaatt gctccatcca ttgcataaac acctgcggcg gcatcgcctt
     5341 cgcaaaaaac catccctggc aatagcgcac gccgcgtttg cgcagccagt taacctgctc
     5401 ctcagtttcg acgccttcag cgatcgtttt taaccccagg ctgtgcgcca gctcgatgat
     5461 gtgttccgca atcaaatgac tggttttgtg ggtggtcagc gtttcaacaa acgatttatc
     5521 gattttcaaa atatcgacat tcaatgattt aaggttatgc aagttagagt agccaatacc
     5581 aaaatcatca attgccactt cgtaaccagc ctggcggaaa gccagaataa tcggcgtcat
     5641 tttttcgaca tcaagaaatg catgctcagt cacttcaaat ttaatttgct gcggacgcac
     5701 cgcgtattgc tctgttttct gattgattcg cgctatcaac cgtgacgtat gaaaatcgga
     5761 ggccgacagg ttaatagaaa catagcgatc ggcatgtgtt gccaagtacg cgcccagatc
     5821 acggaagaca ttatcaataa cataatcagt tatctgttct atcatcccct ccttttctgc
     5881 cagcggaata aactctgccg gattcattat ttgcccctgc tcaccaagcc aacgtaacaa
     5941 cgcttcagcg ccgatacatt tttctgtttt gatatcgatt attggctggt aataaagaca
     6001 aagttgatgt ttttcgaggg cgcgttgcaa tttacgtttg ggagataaat agttttgtcg
     6061 aatacgtagc cagagtaata gcagaacaag actccccaaa ataccggcgg gcaacgcaaa
     6121 aataagatga ttataaaaat gggttataag acgttgatat gaagtcgcaa caatggctgc
     6181 aattgggcgt tttgtcgaat aaactgtcgc atataaatag ccatttcttt gtacggttaa
     6241 atccttcaaa tgaatcagcg gagaaaacgt tgctgccgag gcctctttgc ttaacgagaa
     6301 aaaggttttc gtcaccgtat catacacacc ccattgcaat gtcgggtcat cagacatcac
     6361 ttcactccag aagagagggt tgataaccgc cacataattt ccccgctgca tataggtcat
     6421 tttatagcca gataaaaaag gcgtatcgcg gtaataatag atagaaacgt taggttcacg
     6481 cttataatcg gccggtgcaa tcgtatagcc gtttacaggc gctatcagcg atgagcataa
     6541 aaaatggtta tcacgggcat agatcaattc attaatataa agatagccac gaataatatt
     6601 caacattcgc ttttgatggg ctggagtgca aacttgcccc tgatagcgtt cagctgcatc
     6661 gctcactaaa tctgcctggc gaatgaccag ctcggattta tccagtgcca gttgagcaaa
     6721 ggttcggagc tgattattca cttctgattt tgcccaaagg aaggcaatcc atagcgaaag
     6781 aatgatggga aagagaacta aaaagataat gcccggcaac gccagtaatt ggtgtcgtgc
     6841 acgatgactc attgctatca atttcctgcc tgtttatacc cggccattgc aaggtgaaaa
     6901 ccctgggtac cgacgtcttt agataaatct tacggtataa atagcacttt tgcctgattt
     6961 gaaattgata cctttctaat cgtcacctgg atgaaaataa ttcattgata ttcaaaatta
     7021 atttcctaca ggaatgcaga ggcggcggga agatatgccc ttaatgccac tgaaaaacca
     7081 cataatctgt acagcaataa aagtcacggc ctaatatggc taacaaacaa ttgtgatata
     7141 gttcacaaaa ttaatgaaac aaacagagtg tttcattttt gtgttctgca agccccgcga
     7201 tagccagagt gtaccacgcc tcccgtgaac aacgccgcgc tgtccagggc atcggctctt
     7261 ttcagaggaa ttgtttgatg gctaccctca ccactggcgt ggttcttctt cgctggcaac
     7321 ttcttagtgc cgtaatgatg tttctggcca gcacactcaa catccgtttt cgtcggtctg
     7381 attatgtcgg gcttgcggtg atcagcagcg gtctgggcgt tgtttccgcc tgttggttcg
     7441 caatggggtt gcttggcatc acaatggcgg acatcaccgc tatctggcac aacatcgagt
     7501 cggtgatgat agaagagatg aatcagacac cgccacaatg gccaatgatt ttgacctgat
     7561 acgtagaagc ctccataacg gaggcttctt ttttatggct ggcagatgct ttaatcatcc
     7621 accttaaaac aatataacct attgttttaa tgacaaatca gaacggaatg tcatcatcaa
     7681 agtccatcgg cggctcgtta gacggcgctg ccggagcgga ctgctgcggg cgagactgcg
     7741 cgccgccgct gaactgattg ccaccctgcg gctgctgagg ctgaccccaa ccgccctgcg
     7801 gctgaccacc accgatatta ccacctgccg gagcgccacc accctgacga ccacccagca
     7861 tctgcatggt gccgccaacg ttcaccacga cttctgtggt gtagcgatcc tgaccggatt
     7921 gatcggtcca tttacgggta cgcagctgac cttcgatata aacctgagaa cctttacgca
     7981 gatattcgct ggccacttct gccagtttgc cgaacagcac aacgcggtgc cattcagtct
     8041 gctctttcat ctcgccggtc gctttatcac gccaggattc ggaagtagcc agcgtaatgt
     8101 tggcaactgc gccaccattt ggcatgtagc gtacttccgg gtcctgaccc agattaccaa
     8161 cgagaataac cttgtttacg cctctgctgg ccatgttcgt gtctcctgaa aaaaaatcgt
     8221 tctgaataag tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga
     8281 cctcggttcc gggaaacaaa cctggccaga cattgttata caacactccg ggtattgcat
     8341 tccaatactg tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg
     8401 gtagcaccat gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc
     8461 gaagttcggg gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac
     8521 aagctcattg tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc
     8581 ttatatgccg aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt
     8641 ctgtcactga tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca
     8701 attgagcaga aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc
     8761 cacgactatt tgcgtttgtt gttcgcccgc gttggcgaac cgcgctgtcc ggaccacgac
     8821 gtcccgctgg cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa
     8881 ggcaagcgtc tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa
     8941 acgctggaga acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc
     9001 gatctttccg atccgccgaa actggaactg caaaagaaac acactattga agtggtggtt
     9061 gatcgcttca aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg
     9121 ctggagcttt ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag
     9181 ctgctgttct ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag
     9241 ccgcgactgt tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc
     9301 gtacagcaat atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc
     9361 ggtgcgatcc gtggctggga tcgccgcaac ttctattatt tccagatgct gaaatcgctg
     9421 gcagatcact ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat
     9481 aaagtggtgt tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt
     9541 ggcgatacct ccattcgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt
     9601 tataaagaga cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc
     9661 ccatgcgcca gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag
     9721 aatacgccgc tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac
     9781 aatctcaaac tagctggtca gcgggcgaag attgcagaaa aaatccttaa agagatcggc
     9841 gatcgtctga aattcctcgt taacgtcggc ctgaattacc tgacactttc ccgctcggca
     9901 gaaacacttt ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc
     9961 ctggttggcg ttatgtatgt actggacgag ccgtctatcg gcctgcacca gcgcgataac
    10021 gagcgcctgt tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg
    10081 gagcatgacg aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg
    10141 ggtgttcacg gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct
    10201 gaatcgttga ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc
    10261 gttccggcga atccagaaaa agtgctgaag ctgacaggtg cacgcggcaa caacctgaag
    10321 gacgtgacgc tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc
    10381 ggtaaatcga cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt
    10441 gcgaccatcg ccgaaccggc accgtatcgc gatattcagg ggctggagca tttcgataaa
    10501 gtgatcgata tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat
    10561 accggcgtgt ttacacctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt
    10621 ggttatacgc caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag
    10681 ggcgacggtg tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat
    10741 cagtgcaaag gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc
    10801 atccacgaag tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca
    10861 gctctggcgc gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg
    10921 cagtccgcaa ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg
    10981 tcaaaacgcg gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac
    11041 ttcgccgata ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc
    11101 attgtggtga ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg
    11161 ggaccagaag gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc
    11221 gcggagtgcg aagcatcaca cacggcacgc ttccttaagc cgatgctgta atcgttaagg
    11281 ccgctttctg agcggccttt tcctttcaga gttgcaccag caatttacgt ttttcttccg
    11341 gcagcaaatt caccgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg
    11401 gcagcgaacc gtctagatat acggtgctcc agtgcgcttt attcagatgg cggcttggac
    11461 gcacatcgct gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa
    11521 cagctgggcg attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct
    11581 gcgtcgcttt ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc atgcaatatt
    11641 gtagcaactc cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg
    11701 atccgccgtg gatgcgatgt tcccccagcc agatgccttg ccaggtgccg gtctgaatac
    11761 gccctttatg taacggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag
    11821 gcatatcgtc tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga
    11881 ggaaaaaacg ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag
    11941 aggcggaggt atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag
    12001 ccagctgatt cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg
    12061 tgagcgtctt ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca
    12121 gcaagacagc gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcacgc
    12181 tctcccgctc tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg
    12241 tggcaagggt ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac
    12301 atcgcgtgcg gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc
    12361 ctgcagccat tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt
    12421 catgttggtg gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt
    12481 cgtcgggcta cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat
    12541 cagctggcga gcgacctctt ttggaatgct gaattcatcc cagccattgt tcattttttc
    12601 ccagaacaca ggatttttca gataatcttc gctttctggt gagaaggttt ttttgccgcg
    12661 ccagaagccc gggctggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg
    12721 cggacgccct gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg
    12781 ttcagcaagc ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc
    12841 aacagcggaa ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt
    12901 gcgcatattt ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca
    12961 aaaaccatct gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt
    13021 gatgaaaagc aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttg
//
LOCUS       DAZKHC020000109        13000 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZKHC020000109 DAZKHC020000000
VERSION     DAZKHC020000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.109"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..188
                     /locus_tag="ACHOSM_RS19255"
                     /old_locus_tag="pgaptmp_003851"
     CDS             <1..188
                     /locus_tag="ACHOSM_RS19255"
                     /old_locus_tag="pgaptmp_003851"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560264.1"
                     /translation="PFVPLFYADKGTFFCLSINKFRILSVLNRLKIGILITIITKYCA
                     KPLIRQAKTVQYKKRTV"
     gene            244..1353
                     /gene="livK"
                     /locus_tag="ACHOSM_RS19260"
                     /old_locus_tag="pgaptmp_003852"
     CDS             244..1353
                     /gene="livK"
                     /locus_tag="ACHOSM_RS19260"
                     /old_locus_tag="pgaptmp_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HGU4560265.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1401..2327
                     /gene="livH"
                     /locus_tag="ACHOSM_RS19265"
                     /old_locus_tag="pgaptmp_003853"
     CDS             1401..2327
                     /gene="livH"
                     /locus_tag="ACHOSM_RS19265"
                     /old_locus_tag="pgaptmp_003853"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HGU4560266.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2324..3601
                     /gene="livM"
                     /locus_tag="ACHOSM_RS19270"
                     /old_locus_tag="pgaptmp_003854"
     CDS             2324..3601
                     /gene="livM"
                     /locus_tag="ACHOSM_RS19270"
                     /old_locus_tag="pgaptmp_003854"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HGU4560267.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3598..4365
                     /gene="livG"
                     /locus_tag="ACHOSM_RS19275"
                     /old_locus_tag="pgaptmp_003855"
     CDS             3598..4365
                     /gene="livG"
                     /locus_tag="ACHOSM_RS19275"
                     /old_locus_tag="pgaptmp_003855"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HGU4560268.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4367..5080
                     /gene="livF"
                     /locus_tag="ACHOSM_RS19280"
                     /old_locus_tag="pgaptmp_003856"
     CDS             4367..5080
                     /gene="livF"
                     /locus_tag="ACHOSM_RS19280"
                     /old_locus_tag="pgaptmp_003856"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HGU4560269.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5477..6793
                     /gene="ugpB"
                     /locus_tag="ACHOSM_RS19285"
                     /old_locus_tag="pgaptmp_003857"
     CDS             5477..6793
                     /gene="ugpB"
                     /locus_tag="ACHOSM_RS19285"
                     /old_locus_tag="pgaptmp_003857"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HGU4560270.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSLPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEQSTKS"
     gene            6891..7778
                     /gene="ugpA"
                     /locus_tag="ACHOSM_RS19290"
                     /old_locus_tag="pgaptmp_003858"
     CDS             6891..7778
                     /gene="ugpA"
                     /locus_tag="ACHOSM_RS19290"
                     /old_locus_tag="pgaptmp_003858"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HGU4560271.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSKFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7775..8620
                     /gene="ugpE"
                     /locus_tag="ACHOSM_RS19295"
                     /old_locus_tag="pgaptmp_003859"
     CDS             7775..8620
                     /gene="ugpE"
                     /locus_tag="ACHOSM_RS19295"
                     /old_locus_tag="pgaptmp_003859"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HGU4560272.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8622..9692
                     /gene="ugpC"
                     /locus_tag="ACHOSM_RS19300"
                     /old_locus_tag="pgaptmp_003860"
     CDS             8622..9692
                     /gene="ugpC"
                     /locus_tag="ACHOSM_RS19300"
                     /old_locus_tag="pgaptmp_003860"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HGU4560273.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9689..10432
                     /gene="ugpQ"
                     /locus_tag="ACHOSM_RS19305"
                     /old_locus_tag="pgaptmp_003861"
     CDS             9689..10432
                     /gene="ugpQ"
                     /locus_tag="ACHOSM_RS19305"
                     /old_locus_tag="pgaptmp_003861"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU4560274.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDLIGPNFTAQ"
     gene            complement(10419..10859)
                     /locus_tag="ACHOSM_RS19310"
                     /old_locus_tag="pgaptmp_003862"
     CDS             complement(10419..10859)
                     /locus_tag="ACHOSM_RS19310"
                     /old_locus_tag="pgaptmp_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HGU4560275.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10979..12721
                     /gene="ggt"
                     /locus_tag="ACHOSM_RS19315"
                     /old_locus_tag="pgaptmp_003863"
     CDS             10979..12721
                     /gene="ggt"
                     /locus_tag="ACHOSM_RS19315"
                     /old_locus_tag="pgaptmp_003863"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HGU4560276.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDEQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKDDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12759..>13000)
                     /locus_tag="ACHOSM_RS19320"
                     /old_locus_tag="pgaptmp_003864"
     CDS             complement(12759..>13000)
                     /locus_tag="ACHOSM_RS19320"
                     /old_locus_tag="pgaptmp_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YrhB domain-containing protein"
                     /protein_id="HGU4560277.1"
                     /translation="DDADLPVVITLHGRFSQGWYFCFEAREFLETGDEAARLAGNAPF
                     IIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2868 a         3308 c         3728 g         3096 t
ORIGIN      
        1 cgccctttgt gccgttattt tatgctgaca aaggcacttt tttctgttta tctatcaata
       61 aattcagaat attatctgtt cttaatcgac tgaaaatagg gattttaatc actattatca
      121 caaaatactg cgctaaaccc ttaatcagac aggcaaaaac agtgcagtat aaaaaaagaa
      181 cagtctgatt tgttaacaca taaaaacaaa gcaacacaac atcacgaatg gggatttttg
      241 actatgaaac ggaatgcgaa aactatcatc gcagggatga ttgcactggc aatttcacac
      301 accgctatgg ctgacgatat taaagtcgcc gttgtcggcg cgatgtccgg cccgattgcc
      361 cagtggggcg atatggaatt taacggcgcg cgtcaggcga ttaaagacat taatgccaaa
      421 gggggaatta agggcgacaa gctggttggc gtggaatatg acgacgcctg cgacccgaaa
      481 caagccgttg cggtcgccaa caaaatcgtt aatgacggca ttaaatacgt tattggtcat
      541 ctgtgttctt cttctaccca acctgcatca gatatctacg aagacgaagg tattctgatg
      601 atctcgccgg gagcgaccaa cccggagctg acccaacgcg gttatcaaca cattatgcgt
      661 actgccgggc tggactcttc ccaggggcca acggcggcaa aatacattct tgagacggtg
      721 aagccccagc gcatcgccat cattcacgac aaacaacagt atggcgaagg gctggcgcgt
      781 tcggtgcagg acgggctgaa agcggctaac gccaacgtcg tcttcttcga cggtattacc
      841 gccggggaga aagatttctc cgcgctgatc gcccgcctga aaaaagaaaa catcgacttc
      901 gtttactacg gcggttacta cccggaaatg gggcagatgc tgcgccaggc ccgttccgtt
      961 ggcctgaaaa ctcagtttat ggggccggaa ggtgtgggta acgcatcatt gtcgaatatt
     1021 gctggcgatg ctgccgaagg catgttggtc actatgccaa aacgctatga ccaggatccg
     1081 gcaaaccagg gcatcgttga tgcgctgaaa gcagacaaga aagatccgtc cgggccttat
     1141 gtctggatca cttacgcggc ggtgcaatct ctggcgactg cccttgagcg tactggcagc
     1201 gatgagccgc tggcgctggt gaaagattta aaagctaacg gtgcaaacac cgtgattggg
     1261 ccgctgaact gggatgaaaa aggcgatctt aagggatttg attttggtgt cttccagtgg
     1321 cacgccgatg gttcatccac ggcagccaag tgatcatccc accgcccgtg aaatgcgggc
     1381 gggtttagaa aggttacctt atgtctgagc agtttttata tttcttgcag cagatgttta
     1441 acggcgtcac gctgggcagt acctacgcgc tgatagccat cggctacacc atggtttacg
     1501 gcattatcgg catgatcaac ttcgcccacg gcgaggttta catgattggt agctatgtct
     1561 cctttatgat catcgccgca ctgatgatga tgggcattga taccggctgg ctgctggtgg
     1621 cagcgggatt cgtcggcgca atcgtcattg ccagcgccta cggctggagt atcgaacggg
     1681 tagcctatcg cccggtgcgt aactctaagc gcctgattgc gctcatctcg gcaatcggca
     1741 tgtccatctt cctgcaaaac tacgttagcc tgaccgaagg ttcgcgtgac gtggcgctgc
     1801 ccagtctgtt taacggtcag tgggtggtgg ggcatagcga aaacttctct gcctctatta
     1861 ccaccatgca ggcagtgatc tggattgtta ctttcctcgc catgctggcg ctgacgattt
     1921 tcattcgtta ttcccgtatg gggcgcgcct gccgtgcctg cgcggaagat ctgaaaatgg
     1981 cgagcctgct tggcatcaac accgaccggg tgattgcttt gacctttgtt attggcgcgg
     2041 cgatggcggc agtggctggc gtactgctcg gtcagttcta cggcgtcatt aacccctata
     2101 tcggctttat ggccgggatg aaagccttta ccgctgcggt gctcggtggg attggcagca
     2161 ttccaggggc gatgattggc gggctgatcc tggggattgc ggaggcgctc tcttctgcct
     2221 atctgagtac ggaatataaa gatgtggtct cattcgccct gctgattctg gtgctgctgg
     2281 tgatgccgac cggtattctg ggtcgcccgg aggtagagaa agtatgaaac cgatgcatat
     2341 tgcaatggca ctgctctctg ccgcgatgtt ctttgtcctc gctggcgtct ttatgggcgt
     2401 gcaattggag ctggatggca ccaaactggt ggtcgacagc gcttcggatg tccgttggca
     2461 gtgggtgttt atcggcacgg cggtggtctt tttcttccag cttttgcgac ctgctttcca
     2521 gaaagggcta aaaagcgttt ccgggccgaa gtttattctg cccgcgattg atggctccac
     2581 ggtgaagcag aaactgttcc tcgtggcgct gctggtgctt gcggtgtcgt ggccgtttat
     2641 ggtgtcacgt gggacggtgg atatcgccac tctgaccatg atctacatta tcctcggtct
     2701 cgggctgaat gtggttgttg gtctttctgg tctgctggtg ctggggtacg gcggttttta
     2761 cgccatcggc gcttacactt ttgcgctgct caatcactat tacggtctgg ggttctggac
     2821 ctgtttgccg attgccggac tgatggcagc ggcggcgggc ttcctgcttg gttttccggt
     2881 gttgcgtctg cgcggtgatt atctggcgat cgttaccctc ggtttcggcg aaattgtgcg
     2941 catattgctg ctcaataaca ccgaaattac cggcggcccg aacggtatca gtcagatccc
     3001 gaaaccgact ttcttcggcc ttgagttcag ccgtaccgcc cgtgaaggcg gttgggacac
     3061 gttcagtaat ttctttggcc tgaaatacga tccctccgat cgcgtcatct tcctgtatct
     3121 ggtggcgttg ctgctggtgg tgctaagcct gtttgtcatt aaccgcctgc tgcggatgcc
     3181 gctggggcgt gcgtgggaag cgttgcgtga agatgagatc gcctgccgtt cgctgggctt
     3241 aagcccgcgt cgtatcaagc tgaccgcttt taccatcagt gccgcgtttg ccggttttgc
     3301 cggaacgctg tttgctgcgc gccagggctt tgtcagcccg gaatccttca cctttgctga
     3361 atcggcgttt gtactggcga tagtggtgct cggcggtatg ggatcgcagt ttgcggtgat
     3421 tctggcggca attttgctgg tggtgtcgcg cgagttgatg cgtgatttca acgaatacag
     3481 catgttaatg ctcggtggtt tgatggtgct gatgatgatc tggcgtccgc agggcttgct
     3541 gcccatgacg cgcccgcaac tgaagctgaa aaacggcgca gcgaaaggag agcaggcatg
     3601 agtcagccat tattatctgt gaacggcctg atgatgcgct tcggcggtct gctggcggtg
     3661 aacaacgtca atcttgaact gtacccgcag gagatcgtct cgttaatcgg ccctaacggt
     3721 gccggaaaaa ccacggtttt taactgtctg accggattct acaaacccac cggcggcacc
     3781 attttactgc gcaatcagca cctggaaggt ttaccggggc agcaaatcgc ccgcatgggc
     3841 gtggtgcgca ccttccagca tgtgcgtctg ttccgcgaaa tgacggtgat tgaaaacctg
     3901 ctggtagcgc agcatcagca actgaaaacc gggctgttct ctggcctgtt gaaaacgcct
     3961 tccttccgtc gcgcccagag cgaagcgctc gaccgcgccg ccacctggct tgagcgcatt
     4021 ggtttgctgg aacacgccaa ccgtcaggcg agtaacctgg cctatggtga ccagcgccgt
     4081 ctggagattg cccgctgcat ggtgacgcag ccggagattt taatgctcga cgaacctgcg
     4141 gcaggtctta acccgaaaga gacgaaagag ctggatgagc tgattgccga actgcgcaat
     4201 catcacaaca ccactatctt gttgattgaa cacgatatga agctggtgat gggaatttcg
     4261 gaccgaattt acgtggtcaa tcaggggacg ccgctggcaa acggtacgcc ggagcaaatc
     4321 cgtaataacc cggacgtgat ccgtgcctat ttaggtgagg cataagatgg aaaaagtcat
     4381 gttgtccttt gacaaagtca gcgcccacta cggcaaaatc caggcgctgc atgaggtgag
     4441 cttgcatatc aatcagggcg agattgtcac gctgattggc gcgaacgggg cggggaaaac
     4501 caccttgctt ggtacgttat gcggcgatcc gcgcgccacc agcgggcgaa ttgtgtttga
     4561 tgataaagac attaccgact ggcagacggc gaaaatcatg cgcgaagcgg tagcgattgt
     4621 cccggaaggg cgtcgcgtct tctcgcggat gacggtggaa gagaacctgg cgatgggcgg
     4681 cttttttgcc gaacgcgacc agttccagga gcgcataaag tgggtgtatg aactgtttcc
     4741 acgtctgcat gagcgccgtg ttcagcgggc aggcaccatg tccggcggtg agcaacaaat
     4801 gctggcgatt ggtcgcgcgc tgatgagcaa cccgcgtttg ctgctacttg atgagccatc
     4861 gctcggtctt gcgccgatta tcatccagca aattttcgac accatcgagc agctgcgcga
     4921 gcaggggatg actatcttcc tcgtcgagca gaacgccaac caggcgctaa agctggcgga
     4981 tcgcggctac gtgctggaaa acggccatgt ggtgctctcc gataccggcg acgcgctgct
     5041 ggcgaacgaa gcagtgagaa gtgcgtattt aggcgggtaa taacacgttg attgataagg
     5101 agtcaaaaga ctcctttgag acaggtgaca aatgtaaaat tgcctgatgc gctgcgctta
     5161 tcaggcctac tgggtgagtt gcaatatatt gaatttgcac gatcttgtag gccggataag
     5221 gcgtttacgc cgcatccggc atgaaacgat gagtaatctg tagagtttga ttcagacctt
     5281 ctatttttcc gcttatccgt gccccatctc ccattttccc tcacccacgc cgtcaccgcc
     5341 ttgtcatctt tctgacacct tactatctta caaatgtaac aaaaaagtta tttttctgta
     5401 attcgagcat gtcatgttac cccgcgagca taaaacgcgt gaattcgcgc attcggtaca
     5461 acaagagaga taaacgatga aaccgttaca ttatacagct tcagcactgg cgctcggact
     5521 ggcgttaatg gggaatgcac aggcagtgac gaccattccg ttctggcatt ctatggaagg
     5581 ggaactgggt aaagaggtgg attctctggc ccaacgtttt aacgccgaaa atccggatta
     5641 caaaattgta ccgacctata aaggcaacta cgaacagaat ttaagcgcgg ggattgccgc
     5701 atttcgtacc ggcaacgctc cggctatttt gcaggtttat gaagttggca ccgccaccat
     5761 gatggcatcg aaagccatta aaccggtata tgatgtgttt aaagaggcag ggattcaatt
     5821 cgatgagtcg cagtttgtgc cgacggtttc cggttactac tccgacagca aaacgggcca
     5881 cttactctcc ctgccattca acagctcgac tcccgttctc tattacaaca aagacgcctt
     5941 caagaaagcg gggttagacc cggaacagcc gccgaaaacc tggcaggatc tggcggacta
     6001 ttccgcaaaa ctgaaagcct ccggtatgaa gtgcggctac gccagcggct ggcagggctg
     6061 gatccaactg gaaaacttta gcgcctggaa cggtctgccg tttgccagca aaaacaacgg
     6121 ctttgacggc acggatgcgg tgctggagtt caacaagccg gagcaggtga aacacatcgc
     6181 catgctcgaa gagatgaaca agaagggcga tttcagctac gtcgggcgta aggatgaatc
     6241 caccgagaag ttctataacg gtgattgcgc gatgaccacc gcctcttccg gttctctcgc
     6301 taatattcgt gagtacgcca aatttaacta tggcgtaggc atgatgcctt acgacgccga
     6361 tgcgaaagat gcaccgcaaa acgccattat cggcggggcc agcctgtggg tgatgcaggg
     6421 caaagataaa gaaacgtata ccggcgtggc gaagttcctc gacttcctcg cgaagccaga
     6481 aaacgctgcc gagtggcatc agaaaaccgg ttatctgcca atcaccaaag cagcgtatga
     6541 cctgacccgt gagcagggct tttatgagaa aaacccaggg gcggataccg cgacgcgtca
     6601 gatgctgaac aagccgccgt tgccgttcac caaagggctg cgtctgggca acatgccgca
     6661 gatccgcgtg attgtggatg aagagctgga gagcgtgtgg accggtaaga agacaccaca
     6721 gcaggcactg gataccgccg ttgagcgtgg aaatcagttg ctgcgccgct ttgagcagtc
     6781 tacgaagtct taatcagtgt aatgtcggat gcgtttcgct tatctgacct ggcatcgcgt
     6841 gtaggccgga taagcgaagc gcatccggca cagttcagga attaaccgta atgtcatcat
     6901 cccgtccggt gttccgctcg cgctggctgc cttatctgct ggtcgcgccg cagctcatca
     6961 tcaccgttat cttttttatc tggcctgcgg gcgaagcgtt gtggtactcg ctgcaaagcg
     7021 tcgatccgtt tggtttctcc agcaagtttg tcggcctgga taacttcgtc gcgctgtttc
     7081 atgacagcta ctatatcgac tccttctgga cgacgataaa attcagcacc tttgtcaccg
     7141 tcagcggttt gctggtgtca ctgttcttcg cggcgttggt ggagtacatc gtgcgcggca
     7201 gccgtttcta tcaaaccttg atgctgctgc cgtatgccgt ggctcccgcc gttgccgccg
     7261 tattgtggat cttcctgttt aaccccggtc gtgggctgat cacccatttt ctcgctgagt
     7321 tcggttatga ctggaaccat gcgcagaaca gcggtcaggc aatgtttctg gtggtgtttg
     7381 cctcggtgtg gaagcaaatc agctacaact ttctgttctt ctatgccgcg ctgcactcca
     7441 ttccccgttc attgattgaa gctgcagcca tcgacggtgc aggcccaatt cgccgcttct
     7501 ttaagattgc gctgccgctt atcgctccgg tgagtttctt cctgctggta gtgaacctgg
     7561 tgtatgcctt cttcgacacc ttcccggtga tcgacgccgc cacgtccggc gggccggttc
     7621 aggccaccac gacgctgatt tataagatct accgcgaagg ttttaccgga ctggatctgg
     7681 cttcgtctgc cgcacagtcg gtggtgttga tgttcctcgt catcgtgctg acggtggtgc
     7741 agttccgcta tgttgaaagc aaggtgcgtt accaatgatt gagaaccgtc cgtggctgac
     7801 gatattcagc cataccatgc tgatcctcgg gatcgcggtg atcctcttcc cgctgtacgt
     7861 ggcgtttgtc gcggcgacgc tggataaaca ggccgtctat gccgcgccga tgacgctcat
     7921 ccccggcaca catctgctgg aaaatatcca caacatctgg gttaacgggg taggtacgaa
     7981 tagcgcgccg ttctggcgga tgttgcttaa cagctttgtg atggcgttca gcattacgct
     8041 cggcaaaatt accgtctcga tgctctcggc atttgccatt gtctggtttc gttttccgct
     8101 acgtaacctc ttcttctgga tgatttttat caccctgatg ctgccggttg aagtacgtat
     8161 cttcccgacg gtggaagtca tcgccaacct gaagatgctc gacagctacg ccggtttaac
     8221 gctgccgctg atggcctcgg cgaccgccac gtttctgttt cgccagttct ttatgacgct
     8281 gccggatgaa ctggtggaag cggcgcggat cgacggtgcg tcaccaatgc gcttcttttg
     8341 cgacatcgtt tttccgctct ccaaaactaa tctggcggcg ctgtttgtga tcacctttat
     8401 ttacggctgg aaccagtatt tgtggccgtt gttgattatt accgacgtgg atctcggcac
     8461 caccgtggca gggatcaaag ggatgatcgc cactggcgaa ggcaccacgg aatggaactc
     8521 agtgatggcg gcgatgttgt taaccctgat cccaccagta gtgattgttt tagtgatgca
     8581 gcgtgccttc gtgcgcggcc tggtcgatag tgagaaataa gatggcagga ctgaaattac
     8641 aggcagtaac caaaagctgg gatggcaaaa cccaggtcat taaaccgctg acccttgatg
     8701 tggcagatgg cgaatttatc gtgatggtcg ggccgtctgg ctgcgggaaa tcgacgctgc
     8761 tgcgcatggt tgccgggctg gagcgggtga ccgaaggcga tatctggatc aacgaccagc
     8821 gcgtgaccga aatggagccg aaagatcgcg ggattgcgat ggtgttccag aactacgcgc
     8881 tttatccgca tatgagtgtt gaagaaaaca tggcgtgggg gctgaaaatt cgcggcatgg
     8941 gcaagcagca aattgccgag cgcgttaaag aagcggcgcg cattctggaa ctggacggtc
     9001 tgcttaagcg tcgcccgcgt gagctatccg gcggtcagcg ccagcgtgtg gcgatgggcc
     9061 gcgcgattgt gcgcgatccg gcggtgttct tgtttgatga gccgctctct aacctcgatg
     9121 ccaagctgcg cgtgcagatg cgtcttgaac tgcaacagtt gcaccgtcgc ctgaaaacga
     9181 cttcactcta cgttactcac gatcaggttg aagcgatgac gctcgcccag cgagtaatgg
     9241 tgatgaacgg cggcgttgcc gaacagattg gcacaccagt tgaagtctac gaaaagcccg
     9301 ccagcctgtt tgtggcgagt tttatcggca gcccggcgat gaacctgctg acaggccgcg
     9361 tgaataatga aggcacgcat ttcgaactgg acggcggtat cgtgctaccg ctaaacggtg
     9421 gctaccgtca gtatgccggg cgtaaaatga ctctcggcat tcgcccggaa catatagcgc
     9481 taagctcgca ggcagaaggt ggtgtgccgc tggtgatgga cacgctggag atcctcggcg
     9541 cagataacct ggcgcacgga cgctggggcg agcagaagct ggtggtgcga ctggcgcatc
     9601 aggagcgccc gacggcaggc agcacgctgt ggctgcattt ggcggaaaat cagctgcatc
     9661 tttttgatgg tgaaacagga caacgagtat gagtaactgg ccttatcccc gcatcgtcgc
     9721 tcatcgtggc ggcggtaagc tggccccgga aaacaccctg gcggcaatcg acgtcggggc
     9781 aaaatacggt cataagatga tcgaatttga cgcgaagtta tcgaaagatg gcgagatctt
     9841 cctgctccat gacgacaatc tcgaacgcac cagcaacggc tggggcgtcg cgggtgaact
     9901 gaactggcag gatttactgc gcgtggatgc gggcagttgg tacagcaaag cgtttaaagg
     9961 tgagccgctg ccgttgcttt cgcaggtggc ggaacgctgt cgcgaacacg ggatgatggc
    10021 gaatatcgaa atcaaaccca ccactggcac tggaccatta acgggcaaaa tggtggcgct
    10081 ggcggcacgc caactgtggg ccggtatgac gccgccgctg ctgtcatcgt ttgagattga
    10141 tgctttagaa gctgcacaac aggcggcacc ggaactgccg cgtggtttat tgcttgatga
    10201 gtggcgcgac gactggcgcg aactgaccgc gcggctgggc tgcgtctcta ttcatctcaa
    10261 tcataagtta ctcgataaag cgcgagtgat gcagttgaaa gacgccggat tacggattct
    10321 ggtttatacc gtcaataaac cccagcgcgc ggcagagttg ctgcgctggg gcgtggattg
    10381 catctgtacc gatgcgattg acttgattgg tccgaacttt acggcccaat agttttcaac
    10441 ggaatatcag gctgcggcgt gctgggctga ttcagcatgt cgccgtttct cctttccggc
    10501 agcatatgct gctgattcaa cgaactatcc ggattacggt tgctgtttaa catcccgccg
    10561 ttggtgttgg gcagcatttg ctgccgggcg ggatttcgct cccccggctg tgactgcaac
    10621 acccgctgag aattattgtt tatctggttt tctaaatgct gctgttgcaa ctgagtttgc
    10681 gttttcagtt gctgattcag catccctttt tgctggattt gctgcgtttg catctgggtt
    10741 tgcatccgct gctggctggg gatctgatat cccggctggt tggggttatt cagagtatta
    10801 atgggctgtg caaagccgac aaacggcagg agtgccgtaa gaatcagaag tcgtttcatc
    10861 gcatatcctc ctctgaagat atcctttaag tttactcgct tcccgacaaa acgatgatta
    10921 attcagagtt atataccagg cttagctggg gttgcccctt aatctctgga gaataacgat
    10981 gataaaaccg acgtttttac gccgggtagc cattgctgct ctgctctcag gaagttgttt
    11041 tagcgccgcc gccgcgcctc ctgcgccgcc cgtctcgtat ggtgtggagg aagatgtctt
    11101 tcatccggta cgcgcgaaac aggggatggt ggcatctgta gatgctactg ccactcaggt
    11161 gggggtggat atcctcaagg agggcgggaa tgccgttgat gccgccgtgg ctgtgggcta
    11221 cgcgctggcg gtaacgcatc cgcaggcagg gaatctgggc ggtggtggtt ttatgttaat
    11281 ccgctcgaaa aatggcaata ccacggctat cgatttccgc gaaatggcac ccgccaaagc
    11341 gacccgcgat atgttcctcg atgagcaggg caacccggac agcaaaaaat cactcacttc
    11401 gcatctggct tccggcacac cgggtacggt agcaggtttc tcgctggcgc tggataaata
    11461 cggcaccatg ccgctgaaca aagtcgtgca gcccgcgttt aaactggcac gcgatggttt
    11521 tatcgttaac gacgcgctgg ctgacgatct caaaacctac ggtagcgaag tgttgccgaa
    11581 tcacgaaaac agtaaagcta tcttctggaa agagggcgag ccgctgaaaa agggcgacac
    11641 gctggtgcag gcgaacctgg caaagagcct ggagatgatt gctgaaaacg gcccggacga
    11701 attctataaa ggcacgattg cggaacagat cgcccaggag atgcagaaaa acggtggctt
    11761 gatcactaaa gatgatttag cggcctataa agcggtcgaa cgcactccga taagcggcga
    11821 ttatcgcggg tatcaggttt actccatgcc accgccatcc tccggcggga tccatatcgt
    11881 acaaatcctc aatattctgg aaaacttcga tatgaagaaa tacggctttg gcagcgccga
    11941 tgcgatgcaa atcatggcag aagcggagaa atacgcctac gccgaccgct cggaatatct
    12001 tggcgacccg gattttgtca aagtcccgtg gcaggcactg accaataaag cctatgccaa
    12061 atccattgcc gatcaaattg atatcaacaa agcgaagccg tccagtgaga ttcgccctgg
    12121 caagcttgcg ccttatgaga gtaatcaaac tacccattac tctgtagtgg ataaagacgg
    12181 taacgcggtg gcggtgacct atacgctgaa caccaccttc ggtacgggta ttgtcgcggg
    12241 cgagagcggt attctgctta ataaccagat ggatgatttc tccgccaaac cgggcgtacc
    12301 gaacgtttac gggctggtgg gcggtgatgc caacgccgtc gggccgaaca aacgcccgct
    12361 gtcgtcgatg tcgccgacca ttgtggtgaa agacggtaaa acctggctgg ttaccggtag
    12421 cccaggcggt agccggatta tcaccacggt gctgcaaatg gtggtgaata gcatcgatta
    12481 tggcatgaac gtcgccgaag cgaccaatgc gccgcgtttc caccatcagt ggttgccgga
    12541 cgagctgcgt gtcgaaaaag ggtttagccc ggatacgctc aagctgctgg aagcaaaagg
    12601 tcagaaagtg gcgctgaaag aggcgatggg cagtacacaa agcattatgg ttgggccgga
    12661 cggtgagttg tacggcgcat ccgacccgcg ctcggtggat gatttaacgg cggggtacta
    12721 aggttagcgg ccctcttcgt gggaagaggg ctattttgtc agggcaagcc gaaggtagcc
    12781 ttttttattt cgtaatcctg tagatattct tccagcggct ttgccgttcc cagagaatga
    12841 atttcaccac tgtctttatc aataataaaa ggtgcgttac cagctaagcg cgcggcctca
    12901 tctccagttt cgagaaattc tcgtgcttcg aaacagaaat accagccctg gctaaagcgt
    12961 ccatgtagag taatgacgac cgggagatct gcatcatcaa
//
LOCUS       DAZKHC020000110        12982 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZKHC020000110 DAZKHC020000000
VERSION     DAZKHC020000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.110"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            169..244
                     /locus_tag="ACHOSM_RS19325"
                     /old_locus_tag="pgaptmp_003865"
     tRNA            169..244
                     /locus_tag="ACHOSM_RS19325"
                     /old_locus_tag="pgaptmp_003865"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Ile,seq:cat)
     gene            complement(344..1189)
                     /locus_tag="ACHOSM_RS19330"
                     /old_locus_tag="pgaptmp_003866"
     CDS             complement(344..1189)
                     /locus_tag="ACHOSM_RS19330"
                     /old_locus_tag="pgaptmp_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4560278.1"
                     /translation="MSAITVSRPEVVNGHTDVICSTSVSHILAVRKSSLLQIDTLIRQ
                     LVEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDCEIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANIWSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQADGLADLERMLHLFSGKPLPDNR
                     ENITLRLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     QLSAI"
     gene            complement(1274..1471)
                     /locus_tag="ACHOSM_RS19335"
                     /old_locus_tag="pgaptmp_003867"
     CDS             complement(1274..1471)
                     /locus_tag="ACHOSM_RS19335"
                     /old_locus_tag="pgaptmp_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU4560279.1"
                     /translation="MKLLTTETALDILIAWLLDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRILRQLQFLQQKR"
     gene            complement(1491..1979)
                     /locus_tag="ACHOSM_RS19340"
                     /old_locus_tag="pgaptmp_003868"
     CDS             complement(1491..1979)
                     /locus_tag="ACHOSM_RS19340"
                     /old_locus_tag="pgaptmp_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4560280.1"
                     /translation="MNLSLTLEADSVNVQALNMGRIVVDVDGVNLAELINVACDNGYP
                     LRIVDESDRTSAERTPPFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTGAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            complement(1976..2356)
                     /locus_tag="ACHOSM_RS19345"
                     /old_locus_tag="pgaptmp_003869"
     CDS             complement(1976..2356)
                     /locus_tag="ACHOSM_RS19345"
                     /old_locus_tag="pgaptmp_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4560281.1"
                     /translation="MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFS
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGVPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGRHPEEAKQ"
     gene            complement(2445..2813)
                     /locus_tag="ACHOSM_RS19350"
                     /old_locus_tag="pgaptmp_003870"
     CDS             complement(2445..2813)
                     /locus_tag="ACHOSM_RS19350"
                     /old_locus_tag="pgaptmp_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4560282.1"
                     /translation="MSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     DDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVALYAKGLTCDAD
                     TLGSCGYVYLAVYPTPDMKK"
     gene            complement(2888..3109)
                     /locus_tag="ACHOSM_RS19355"
                     /old_locus_tag="pgaptmp_003871"
     CDS             complement(2888..3109)
                     /locus_tag="ACHOSM_RS19355"
                     /old_locus_tag="pgaptmp_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU4560283.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGSYVRLMSVTLN"
     gene            complement(3178..3654)
                     /gene="radC"
                     /locus_tag="ACHOSM_RS19360"
                     /old_locus_tag="pgaptmp_003872"
     CDS             complement(3178..3654)
                     /gene="radC"
                     /locus_tag="ACHOSM_RS19360"
                     /old_locus_tag="pgaptmp_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGU4560284.1"
                     /translation="MQQLSFLPGEMTPGERNLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGRQVFSFAEHGLL"
     gene            complement(3670..4143)
                     /locus_tag="ACHOSM_RS19365"
                     /old_locus_tag="pgaptmp_003873"
     CDS             complement(3670..4143)
                     /locus_tag="ACHOSM_RS19365"
                     /old_locus_tag="pgaptmp_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4560285.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATLIPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWILFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(4237..4482)
                     /locus_tag="ACHOSM_RS19370"
                     /old_locus_tag="pgaptmp_003874"
     CDS             complement(4237..4482)
                     /locus_tag="ACHOSM_RS19370"
                     /old_locus_tag="pgaptmp_003874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560286.1"
                     /translation="MKHIPEGVIQVPESPDAPEALPGGRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWSSVCDRNGYAYGCPEFQPNIIHP"
     gene            complement(4482..4922)
                     /locus_tag="ACHOSM_RS19375"
                     /old_locus_tag="pgaptmp_003875"
                     /pseudo
     CDS             complement(4482..4922)
                     /locus_tag="ACHOSM_RS19375"
                     /old_locus_tag="pgaptmp_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            complement(5041..6819)
                     /locus_tag="ACHOSM_RS19380"
                     /old_locus_tag="pgaptmp_003876"
                     /pseudo
     CDS             complement(5041..6819)
                     /locus_tag="ACHOSM_RS19380"
                     /old_locus_tag="pgaptmp_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243194.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
     gene            complement(7332..7712)
                     /locus_tag="ACHOSM_RS19385"
                     /old_locus_tag="pgaptmp_003877"
                     /pseudo
     CDS             complement(7332..7712)
                     /locus_tag="ACHOSM_RS19385"
                     /old_locus_tag="pgaptmp_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            complement(7932..8342)
                     /locus_tag="ACHOSM_RS19390"
                     /old_locus_tag="pgaptmp_003878"
     CDS             complement(7932..8342)
                     /locus_tag="ACHOSM_RS19390"
                     /old_locus_tag="pgaptmp_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560287.1"
                     /translation="MKTIIKTLLVAVTVLFSVNVAAKTIKLPAGIKYVNSTDEFYCTE
                     IDGLHCQTKNLFKYKGNRYVFVIELGGAWCYDNPMSVLNLETWKAQRLEYNDKRLCSG
                     NNEPFFEIKNGVPTVGIIDTSEKPVIVAQDKLKI"
     gene            complement(8358..9035)
                     /locus_tag="ACHOSM_RS19395"
                     /old_locus_tag="pgaptmp_003879"
                     /pseudo
     CDS             complement(8358..9035)
                     /locus_tag="ACHOSM_RS19395"
                     /old_locus_tag="pgaptmp_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9171..10241)
                     /locus_tag="ACHOSM_RS19400"
                     /old_locus_tag="pgaptmp_003880"
     CDS             complement(9171..10241)
                     /locus_tag="ACHOSM_RS19400"
                     /old_locus_tag="pgaptmp_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HGU4560288.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLLQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRRAFPDTTILEIRPR
                     KRLKHTGDGGNSGGLLSFASAHTDAWRQQGYEDTMLTMEHVRKPLAARQALSRSEAVL
                     QKSLDITEEADLALRNAMARIK"
     gene            complement(10238..11143)
                     /locus_tag="ACHOSM_RS19405"
                     /old_locus_tag="pgaptmp_003881"
     CDS             complement(10238..11143)
                     /locus_tag="ACHOSM_RS19405"
                     /old_locus_tag="pgaptmp_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HGU4560289.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTELTTSLASTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADHVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            complement(11140..12432)
                     /locus_tag="ACHOSM_RS19410"
                     /old_locus_tag="pgaptmp_003882"
                     /pseudo
     CDS             complement(11140..12432)
                     /locus_tag="ACHOSM_RS19410"
                     /old_locus_tag="pgaptmp_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
     gene            12497..12757
                     /locus_tag="ACHOSM_RS19415"
                     /old_locus_tag="pgaptmp_003883"
                     /pseudo
     CDS             12497..12757
                     /locus_tag="ACHOSM_RS19415"
                     /old_locus_tag="pgaptmp_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            12739..12825
                     /locus_tag="ACHOSM_RS19420"
                     /old_locus_tag="pgaptmp_003884"
                     /pseudo
     CDS             12739..12825
                     /locus_tag="ACHOSM_RS19420"
                     /old_locus_tag="pgaptmp_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12822..>12982
                     /locus_tag="ACHOSM_RS19425"
                     /old_locus_tag="pgaptmp_003885"
     CDS             12822..>12982
                     /locus_tag="ACHOSM_RS19425"
                     /old_locus_tag="pgaptmp_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HGU4560290.1"
                     /translation="MRRTFTAEEKDSVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKR"
BASE COUNT         3019 a         3361 c         3166 g         3436 t
ORIGIN      
        1 ttgcttgttg actgcgagta tcgactttgc gaatggaggc aatgtcgggt tttatgtcgc
       61 atgtattgac gacccaggat ggatgagaac gcataaaaat ctgttattta catcataaga
      121 tcatcatgtt tattgcgtgg tttataagta ttgttattgc atacttaagg ccctttagct
      181 cagtggttac agcaggcgac tcataatcgc ttggtcgttg gttcaaaccc aacaggggcc
      241 accatataca acaaagggtt agctaataaa tcagctaacc ctttttcttt tacctttgaa
      301 ttgggtcagg taatgggtca ggtaagcttt ttctccatct gactcagatg gctgatagtt
      361 gccccggata atgcttcgca atgatgtcat ttagccgatc aaccagttcc ggttttctga
      421 acgtgatgtg tgcggagccc ttcttaaagt atctgatgct gaacatctca tcttcatagt
      481 cctctttacc ctgaacagac tgaatgtgat catccagacg aagggtgatg ttttcccggt
      541 tgtcggggag cggtttgccg ctgaacagat gcagcattct ttccaggtcg gcgagtccat
      601 ctgcctgttg cccggtgata aggtgaaatc cccatctgtc ccacctcacc aggttgttga
      661 cgataatttt actgccaaat ttacaggggg agttggtttt gtaattccag ctcagccctc
      721 tgaagacgtt gatcactcct cgctcaaaca cttcatcttt attctggtgc agttgctcaa
      781 atgtgctcca aatgttcgct tcactgattt ccggaaaatt atcgtactcc agtgatcggt
      841 accatgtatc acgggcctgc gcatccatta atgacagcat cccggaacgt tgcatcaggt
      901 cacgccagat ttcgcagtca agattgcggg tgatggcttt cattgcggtt tccggtttct
      961 ccatgagcca gcagccacag cgaaaatcct gtttcatggc ccagtccaga gcagttttac
     1021 ctccaatact ttctgtcagt accgagattt ctaccagttg tcggataagt gtgtcgattt
     1081 gcagcagtga actctttcgt acagccagaa tgtggctgac tgaggtggag cagataacgt
     1141 ccgtatgccc gttgaccact tccggacggg agactgtgat tgcagacata atcgatattc
     1201 cttttcgcag ataaatgaaa acgccagccc tgttcaggct ggcgtcagtg gaatgaagta
     1261 tggtgagtga gactcaccgc ttttgttgca gaaactgcag ttggcgcagg atacggatat
     1321 cttccctggc ctgttcaata cagggcaaca gtgctgccga atccgtttta tcctcatcgt
     1381 tgtcgaagat aatccctgat tcgcagtcaa tattgtccag cagccacgca atcagaatat
     1441 ccagcgccgt ttctgtggtt aataatttca tccagtgtct tttgtcctga ttaccagtcg
     1501 aaagtgggta agcccggcag gcattcagcg ctggcatcca gatgaatgag actgacgcca
     1561 taacgccggg tgagagtgac gaccagacga cggaacgttt ttgacaggcc caggcgctta
     1621 agccgcagaa ccgggtatga ccacgcaccg gtacgtaaca gatatccgct acctgtgaaa
     1681 tgaatccatt ctgattcgcc ggtgtcattc gtctgatgtg acagcgaata caaccaggcg
     1741 ttgtcctttg ccgtgatatg tgcggtactg cagcgtatgc cggtcagggc agcaaatggt
     1801 ggcgtgcgct ctgctgatgt ccggtcagat tcatcaacaa tacgaagggg gtagccattg
     1861 tcgcaggcca cgttaatcag ttcagcgaga ttaacaccat cgacgtcaac gacaatgcgt
     1921 cccatgttca gcgcctgcac gttaacgctg tctgcttcca gcgtcaggga aaggttcatt
     1981 gtttcgcctc ctccggatgc ctgcccaggg taatgttatt taccgttctg taattgtcgc
     2041 gggtcatcag gccggttgcc ctgcgggccc ggaggatatc gatgctgttg attaactgcg
     2101 acgggactcc tgcgctgaat cccggctggt cagttcgtac cagcgcgtat ttttcaacga
     2161 gaaagttcac cgcgtcacac agtgaaatcc ctgcctcaat atgctgctca atcacacgtt
     2221 catcagagaa cggtgtgtca ttcagcgtga ggccatagtg ctggtctagc agtcgggtga
     2281 gcagtgtctg ccagatttca accggagacg ggcagcgcga tgccgcccgt acgtgagtgt
     2341 cgggtaatgt tttcatactg aagattttcc tgatatgcaa agataaaaat gggaaagtgg
     2401 cgtggcgaaa gcaccaggcc ggagcagagg gttattccgg tggtttactt tttcatatcg
     2461 ggcgtcggat aaacagccag ataaacgtaa ccacaggagc cgagggtgtc ggcatcgcag
     2521 gtcagccctt tcgcatacag tgccacggta tgctgatggc ggggattcag ttcaccgctg
     2581 gtgagcatga gttccagttg tttcatcagc agcggaaagg cctggtctag gtggtacgca
     2641 tccgcgtcgc tgaaccggcc tctgataccg gcgcggtcgt caaggtaatg caaccggtta
     2701 ccctcctgca ccagacgtgc cccgaaacag ggcgtcacgg tgcagggcag tccccaccag
     2761 gggcggtcgt gattgtcgtt gggataattt gtctcatgga gtgtgtctga cacgataaaa
     2821 tcctcacaaa aaatcagtta agaatggtcc ggtggcagcg ataattctgc tcatcatttc
     2881 tcctgactca gttcagtgtg acgctcatca gccggacata cgagccaaaa ctgtccttac
     2941 ggcgttctgc aaacacggca agaacgccgg gaatatcctg tacttcgcga ccggtatacg
     3001 cctcagcgct accgtgccag cgatacttac cggtacagaa cggaaaaaga cgggatgcag
     3061 gatgctgttg gtgaatacgc atggcttcac cacgggtgat gattctcatg atgtgatacc
     3121 tctgaagaca gaagataaaa gcgaaaacag gtatgatgtg gtggtgacgg tgacgggtta
     3181 aagcagacca tgttcggcaa aggagaaaac ctgtctgcca ccaactatca gatggtccgg
     3241 tacccggata tccaccaggg ccagtgcctg taccagacgc tccgtaataa gccggtctgc
     3301 cttactgggt gtgacttcac cggacgggtg attgtgtgcc agcaccacgg cagcggcatt
     3361 gtgatacagg gcgcgtttaa tcacttcccg gggatggact tccgtgcggt tgatggtgcc
     3421 ggtgaagagg gtttcaccgg caatcagctg attctggttg ttcagataca gcacccggaa
     3481 ttcttcacgc tccagtcccg ccatgttcag aatcagccat tcccgtgccg cacgggtgga
     3541 ggtgaaggcc acgccgggtt catgaagatg gcggtccagg gttttcaggg cccgctgaat
     3601 gaggttgcgc tcgccgggcg tcatctctcc gggcagaaag gaaagctgct gcattgtccc
     3661 ggcctccatt cagtcgatga tgcgcataat ggcgctgcat tccggatgct gcagggcgta
     3721 atcccgcaac cggtaataat ggaccgtcat ggcataacat tccgtacgac aggcatgatg
     3781 gctgtacgtc atcagacagg cagcaatgcc ggcggcttcc gggctcattt cagcgcggtt
     3841 accgttcatg gcattgaaca gtatccaggt ttcgtcatca tcgtcatccg gttcgggtgc
     3901 cataaatgcg ccaccgttgt tcagggtgta cagattccag ataccaccgc agtagtcttc
     3961 acacagacgg tccatccagc cgaagacacg gggctccagg gtcacccact gtggaatgag
     4021 gccaaaatgc tgcggccaga agctgatgcg ctgttcatca gggatgaggg tggcaaccag
     4081 ttgttgcagg ccagtctccg gtgcacaggc tgaagaggtg gtgttctgag acaatgtttt
     4141 catgagtgaa atccttgtaa tgtgaataaa tgagagggag taaaggccgg gggctgacaa
     4201 catcacgttc atcactgacc gtgagccccg gtaatatcag ggatgaatga tattcggctg
     4261 aaattccgga cagccgtacg cgtaaccgtt ccggtcacag acggatgacc agatgccatt
     4321 gcctgtactt agtctgcaca gactgagttc tccgggacgg taaagcagac gggtacacca
     4381 ggcgcacagg tcatcatcag aaatggaacg cccccccggc agcgcctctg gggcgtcggg
     4441 tgattctggt acctgaatca cgccttcagg gatatgtttc atcagcgcat actctccagc
     4501 agcgtttctg ccatcaccca caatgcgcgg ttgagcttaa tatcggtgtc gatgctgtga
     4561 atggcccggg tatggatacg ttttcctctg gcactgcgac cggaaatccc gcctttcagc
     4621 atattctcct ggatggtctg ataagcactc cacaggtcct taccgtaatc ctcccggcgt
     4681 cgtggcgtca gaatgtcggc ggtggtgacg ggctgatgtt cgtcaccata acggtaagtc
     4741 agtgccgcct gtgccagcgc ctggcgtgcc ggtggtggca ggaccagcga ctgcatggca
     4801 tcacgtttct cttcaatccg gtcaaaaacc cccaccacct cgtaagctcc ttcgataact
     4861 ttctccacta catttccccg gtgcggaaca cgcacttccc ccagagactg gccacagacg
     4921 cacgtagggt aggctgccga cgagttgctg tattggcact tttccgtcag cccccctccg
     4981 aaccggacgt gcgactttca tcgcatccgg ctctccagtt acaacaaatc cgcaaggtaa
     5041 ttagagtttt ccctgatgaa tcgcgacatg gcattggtga catacaacca gtgtcttacg
     5101 tcgccttgct gccatccttc tttgtgatgc tgtgggcgtc cttccccgtt tcgcaagatc
     5161 tttcagtgcc cgaatgtggt gaacctgtat attttgggtt gagccacata ttgcacattc
     5221 agaagccagt atggctttca gtcgctcaac gcgcccataa aagagcaagt ctttctcatc
     5281 aactaacaca atattccggt aacttttgtt acgggccaga gatattccgc cccatgttgc
     5341 agtcaatggt gctttattaa ctctatctat tcttatctgt agacatttgt aagatttacc
     5401 attagtcatc atgcatcgtg ttccatattt ttgatagatc cggctaaccc acgttttatg
     5461 tttgttagcc agagttaaca ttagggaatg ttccatgatg cctttcagac gagattattt
     5521 tttacttctg tcacaggcta gttgataata gttagctaaa ccacgaaaac gggtctggaa
     5581 attatgaacg atagtgtaat cactctcctc cagtaattcc ggtcgtctcc ttactcgtcc
     5641 tttaagaaag taacgtttgc tgtgttctct cactacatcg gcgggaacaa gtagccggat
     5701 gtcgccattg acactgcgtc ttttctttct gttacggaac gtatcacatt gaccaacctg
     5761 aatatcgtat ccaagaaaac gggctttatc tgttcttcca tgggtaataa gcgttttctc
     5821 ctgagaaact tcaagactca gattattctg aagaaacagg gttatttcct ttttgatttc
     5881 ctcagcttct gattttgggc cagagaatcc gagcaggaag tcgtctgcat atctcacata
     5941 tcgcaaccgc cgataatgcg ggtcaaaagg gtcttttgac ggcaacgttt gtttcttctt
     6001 acgtgccgct ctggatatat ccagtctccc ccttttacga ttgtaatgtt caataccatt
     6061 ctgaacttca tactctcgat tccttgctct ggatttaccc ttgttatatt taggcaataa
     6121 cacgtgttcc acgtactggt caagtttatt caggtatata ttagagagca acgggcttat
     6181 tattccaccc tgaggtgttc cactccaggt tttatggcat ttccattcct ccatatatcc
     6241 ggagtaaagc agtttctcaa ttaaccggat gaatcgatta tctttaattt gttcccgaag
     6301 tatatgcagc aatatttcgt gatcaatatt atcgaaacac cctttaatat ccccttcaat
     6361 aaaccatgtg gaagccgtcc atgttttggc gatgactgtc agtgctgtat gacaaccacg
     6421 ctgtgggcga aaaccgtgag agaactggct gaactgaggc tcgtaatagg cctcaaggat
     6481 catacgcatg acctcctgga gcagcttatc actccatgtt ggcaggccaa gaggacgcat
     6541 tttcccgttc tttttcttaa tatagatcct tctggctggg gagaacttat atctctcatt
     6601 acgcagaaga ctgataatgt catggatctt tttgtgcgac attccatcaa aggtttcgtc
     6661 ggtaactcct tttgtcattg cccctttgtt gctaccgatt tttgcgtagg cagcaatata
     6721 taactccggg ttaaaaagca tccgataaat ctgatccagc gatttacctt cagcccctct
     6781 cttgcggatg atgcctaata ttgttctggc gtttcgcatc tcgcgtacct ctcaatttta
     6841 aacatcgttg taacctgcca cccttcgcca tgtatccggc tttcccggac ccggactact
     6901 acggtggctc cgttgcccta gggctcgcgc cccttagaca atcccgtgtt tacacggtaa
     6961 ggaacgtaac agcgcaactt aggtgcccta ctcataacct tggtaccctc actgggtacg
     7021 gcttaaccgg ggaatgattg ggacgaccaa cggcatttcg tctgatccgg ttatcatcac
     7081 tcttaaacgt ggtagtgacg gggagtgcct tatcccgctt ggttattagg gttcaggcag
     7141 tgtagctttc accatattgc gcgagtctcc cggaacagtt cagccataca cgttttctgg
     7201 ccctctccgg tttaccgcca tgctattgtc cccttgacct ttcagcctca ggtaaggcgt
     7261 tgactcagtg gtatttcatt ccgaccactc ctgattgaat caggaatccc ttaccacgcc
     7321 gacacggcgc acccgttctg gcagacgaac ctgaagtaac ccggcagcat ctggtagctg
     7381 gaggtaccgt catgagagtt gagcagaata atttcaggga catgttctcc gtttatctct
     7441 ccggcccgcc gcagacgcag catgtgtttt gtgtatcccc gacggcccgg gtcgcgcaca
     7501 cgggtctggc aggcgaagaa tggctggaag ccttcccgct gcagactttc cagtacggtg
     7561 atggtgggga tgtacgtata gttctggctt cgggacgtgt gtttatcttc tccgaaaatc
     7621 cccggcacat ggtgcatcag ctcttcatgt gtcagcggac ggtcacggcg tatctggttt
     7681 gcagcataac caaaacgact ggctaatcgc atactttgct ccttatcggt ggttaagatt
     7741 tactggtgta ataaatgaaa aagccacgtc tcccggagaa gacgtggcct gatgggtgaa
     7801 atgaatgacg tttattgtct gggaagccct tcactggcga gccacgcatt aagcggcttt
     7861 ccggtaacaa tggctggggc tgtatttacc tttgatatta ataatgtgca gagaaacgca
     7921 tggtttactc cttagatttt aagtttgtcc tgagctacaa tgacaggttt ttcagaggtg
     7981 tcgataatac ctacagtcgg gacaccattt ttgatctcga agaacggttc gttattacct
     8041 gaacatagcc gcttgtcatt gtattcaagg cgctgtgctt tccaagtttc caggttgagt
     8101 accgacatag ggttgtcgta gcaccaggca ccacctagtt caataacgaa aacatatctg
     8161 ttacctttgt atttaaacaa attctttgtc tggcaatgca aaccgtcgat ttctgtgcag
     8221 tagaattcat cggtactgtt gacgtatttg ataccagccg gtagctttat tgtttttgct
     8281 gctacgttaa cagagaacag aacggttaca gcaacgagta aagttttgat aatcgttttc
     8341 atgatagact ccttaaatta gttaaccgca gctacgtagt ttttcaccag atacgcgaat
     8401 tcgtggacac caatttcacc aaaccaaatc aggtaaccag ggtcgatgtt gtaatagtag
     8461 ccaacgactc tatgcgtttt tgggttatag agtgttacga taaagtccca gccgcaggcg
     8521 tgggccttac aaccgccacc ttctacccag gtcgttccgt taatgtttac aggatacgca
     8581 ctaccatcag gaccatccat atagccgtct tacccaaaca ttccgtttaa cccaacaggg
     8641 gttaatgctt tgtcgatagc agctctgtag gtagcgtcac cttcgtataa ctcgtacgtt
     8701 gttggacacg ctccaggttg ttcagcccag gctcccggct tacaactaag tgcttgtttt
     8761 acggtggccg cttctgcgtt aaagttaaag cccgtgaaca gaaccgccag aaataacatc
     8821 agggtatgta aacgtttcat tgtaaatatt cttccgttgt aaatggcact ggaaaatatc
     8881 tcatcagtga aaccttccgg aagtttccac cccttgtatg atgtatatcg ccagtatttt
     8941 atttcatccc ggctgtaatt tttatattcc ccggcacgtg caataacgct gaccagagat
     9001 ataaggctca ttaataactt tacaggtaat ttcataatta aatttccagt gagtgaattg
     9061 acaggtgagg ggtaaaactt tctctcaggt aatatatatc tgaaaatatt ttcaataaca
     9121 ccagacagga aaaacaccac cacaggcggt ggcgtttctt tggggcgtct ttatttaatc
     9181 cgggccatcg cgtttctcag tgccaaatct gcctcttccg ttatatccag gcttttctgc
     9241 aataccgcct ctgaccggga cagtgcctga cgtgctgcca gcggtttccg gacatgctcc
     9301 atcgtcagca tcgtgtcctc gtagccctgc tgacgccagg cgtcggtatg tgccgacgcg
     9361 aaactgagca gaccgccgct gttgccacca tcacctgtat gtttcagcct tttccgggga
     9421 cggatctcaa ggattgtggt gtccggaaaa gcccggcgat cccataaaga accgtcactc
     9481 agatgcgaca caatcaccat attgcatccg gcatcgacca gaggcgttac aggggtattt
     9541 ccctgcatat ttcgccatcc tcccatacca ccatcaccga acattgtccc ctgaacctca
     9601 cggggacgga acaacagggg cagcgcggct gacgcaagca gagcctcttt ctgctgtccg
     9661 cggggcagac tctggatatg ctgaaaaacg gcgtttttcg tggttccgac acccagttca
     9721 gcacgaatgc agtcaataat atcctgcatg cccccttctg tggggtacag cgacacatac
     9781 agcggtagcc cgtctgccag agcatcagta tcaagataat gatccatcag cgctgtcagg
     9841 ggctcatctg acaacaacgg ctgagtcatc ggattgtcga agccgttgat tatcgatata
     9901 tggcgaagaa gcgtcgtaag cagtgctcct gcacgtccgg ggatctggca gaggttcatt
     9961 gcctggagca attttttcag caatgaaaag taaaccgatc tgtttaccga cagcacctga
    10021 ttattcccaa gatgatccca gagcgcctcc aggcgtacgg cagcttctga cagatcggga
    10081 gaggccgcga taatggcacc attgaatgcg ccaatgctgg cccctgaaac catgctgatc
    10141 tgtgtaccac actctgccag tgccttaacc actcccgcct gataagcacc caccgccccg
    10201 ccgccggaca gcaccagccc cgttttcatt tctgtactca tgcagccccc ttctgaaata
    10261 tttcatcgac catactttca gagaggcgct ctgcagagca gagacggggc atgcgaaccg
    10321 gtaagtgttc tgcagcctgc gtggttgacc agatcaccgg atgctgctct tcactgcacc
    10381 agtcccccag ccagttaatc gcgtcatgcc actcgttatc tgcctgctcc cggccctgac
    10441 caccaagcca gtccagcgtg ttcagacgca cggttgcact accgccactc tcctgtgcca
    10501 tctgcgttaa tgctttatgt ctgagaatat ccagtagctg gttaacctgg cctgtttcgc
    10561 cctgacgaat cacatcacca aacgcgcccc agcgaagctc ttcaagtgcc acataacaac
    10621 gtccggcagg ggaaaatgac gcatatcgcc cggcgctcca ccatgagaat atctgctcaa
    10681 gatgtaactg cgcccgctga acctggtcaa cacggtggag tttttcttca agatgattca
    10741 gtttctggtg aacatgatcg gccagggtga gtaactgctc ccgcgtatct gcggaataat
    10801 gcgccagcct ggcagtatca ctgaccagtg aactgaccct gtcgtttacc cgggtaatcg
    10861 cgtagtttgt ggaggcaagc gacgttgtca gctccgtcag ccagcgcagg gacgcctcaa
    10921 cgccctgtgc aagcgaagca ttgatggcat tctgccgtgc agcgccttcg ccagtcagtc
    10981 cctcttttaa acgccggaag aacgtgcgct ctccggccgt gcggatgtgc tcctgtgcaa
    11041 catcgatacc gtttgcgaaa tcaaccacaa atttttcagg aagacaggtg agtacctgac
    11101 atacccggtt atcagcaatt tgttgaataa atggtgaagt cacagcattg tctccagctc
    11161 ttccctcagc gccttactgt cagtaatgag ctcaggaacc ctttcattta atgcctgaag
    11221 tcgcccggta agccgttcct gcgcctgctg atcgcgttta tgatcttcag agctctgaat
    11281 cagcgtgtta cgcagctgtt caactttctc cctgaatgta cagaaaaagg catcgatttc
    11341 ctggcggacg ggctggttaa tatcctgctc aatggatgcc tgcagctccc cgaaatatgc
    11401 ccgggtcagt gacatcactt cctctctgac cttgttgata ttgatcacgc tgcggctcac
    11461 atcctcctta taggtttccc agccccattc tttggtgcca atccatccac aaatctttcc
    11521 aatgaagcta tccttccgac ggcgacgggt gattgtctcc gttctttcct ccataagcgc
    11581 actcagattt gtttttactg cgagccgggt ctgtaactgg ctgactgccg gaaaactgat
    11641 ttttacggta aagccctcat cctccaggcg ggcattgatg cgaccggcaa tgttttcagt
    11701 ggcatacagg gccgtgccac gaaaacctgt ttcaataccc ctgacgattt tctccagctc
    11761 aggccggaac tgtgcttcag cctcacggtg cagggtggtc acagttgact caatttcctc
    11821 tatatattca attgcttccc tgcgattatt tagtgttatc tcggggtttt tcgggtcaaa
    11881 gtctcgtttc ctgtaatctt cccagcccgg gttgttgata ccggaccatt gagaaatcat
    11941 tccccataat ccggaccggg accttcgttg ttctgcgtct tcttcctcca gcatttgctg
    12001 ttctttgcgt ttaccggttc tgaaatagtg attcagctcc tcttccagtg aggtgcagac
    12061 ccccgtgagt aattcccggg ttctggcatc gatctcctcc atggccattc tgacatcagc
    12121 ggtcacctgc tcctgacagt ccgcgatttc ccggatatcc gcaagcaggc tggtgatatg
    12181 cgactgcagt gactgaatgc tttgctgtag cccctgatgg cgtaatgaca gatattcgct
    12241 gacattttcc gcattctgca tcagttttgc ggcggcagaa tcaaccgcca gtgccgccgc
    12301 tctggaatgg gagctctgta tgacttccgt tatcagttga tcgataagtg aaccttccca
    12361 caggtctgtc gccccttcgg ttgccatttc actgtcttta cagacgtatt cttttttcat
    12421 gcgaccgaag gctgaggtag cctgagttta acggacactc cttcctgaaa tagaatggca
    12481 tcagaaggag ctaataatga gcagaaaaac ccaacgttac tctaaagagt tcaaagccga
    12541 agctgtcaga acggttcttg aaaatcaact ttcgatcagt gaaggcgctt cccgattatc
    12601 tcttcctgaa ggcactttag gacaatgggt taccgccgcc agaaaagggc tcggtactcc
    12661 tggttcccgc acggtggctg aactggaatc tgaaattctg caactgcgta aggcgttaaa
    12721 tgaagctcgc cttgagcgag atatattaaa aaaagcaact gtagattcaa ttggtcaacg
    12781 caacagttat gtgaaaacat ggggttgcgg aggttttttg aatgagacga acatttacag
    12841 cagaggaaaa agactctgtt tttgaactat ggaagaacgg aacaggcttc agtgaaatag
    12901 cgaatatcct gggttcaaaa cccggaacga tcttcactat gttaagggat actggcggca
    12961 taaaacccca tgagcgtaag cg
//
LOCUS       DAZKHC020000111        12568 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZKHC020000111 DAZKHC020000000
VERSION     DAZKHC020000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.111"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            56..1570
                     /gene="caiT"
                     /locus_tag="ACHOSM_RS19430"
                     /old_locus_tag="pgaptmp_003886"
     CDS             56..1570
                     /gene="caiT"
                     /locus_tag="ACHOSM_RS19430"
                     /old_locus_tag="pgaptmp_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HGU4560291.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1601..2743
                     /gene="caiA"
                     /locus_tag="ACHOSM_RS19435"
                     /old_locus_tag="pgaptmp_003887"
     CDS             1601..2743
                     /gene="caiA"
                     /locus_tag="ACHOSM_RS19435"
                     /old_locus_tag="pgaptmp_003887"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HGU4560292.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2872..4089
                     /gene="caiB"
                     /locus_tag="ACHOSM_RS19440"
                     /old_locus_tag="pgaptmp_003888"
     CDS             2872..4089
                     /gene="caiB"
                     /locus_tag="ACHOSM_RS19440"
                     /old_locus_tag="pgaptmp_003888"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HGU4560293.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4163..5716
                     /gene="caiC"
                     /locus_tag="ACHOSM_RS19445"
                     /old_locus_tag="pgaptmp_003889"
     CDS             4163..5716
                     /gene="caiC"
                     /locus_tag="ACHOSM_RS19445"
                     /old_locus_tag="pgaptmp_003889"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HGU4560294.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLIDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5825..6610
                     /gene="caiD"
                     /locus_tag="ACHOSM_RS19450"
                     /old_locus_tag="pgaptmp_003890"
     CDS             5825..6610
                     /gene="caiD"
                     /locus_tag="ACHOSM_RS19450"
                     /old_locus_tag="pgaptmp_003890"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HGU4560295.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6616..7206
                     /gene="caiE"
                     /locus_tag="ACHOSM_RS19455"
                     /old_locus_tag="pgaptmp_003891"
     CDS             6616..7206
                     /gene="caiE"
                     /locus_tag="ACHOSM_RS19455"
                     /old_locus_tag="pgaptmp_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HGU4560296.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7292..7687)
                     /gene="caiF"
                     /locus_tag="ACHOSM_RS19460"
                     /old_locus_tag="pgaptmp_003892"
     CDS             complement(7292..7687)
                     /gene="caiF"
                     /locus_tag="ACHOSM_RS19460"
                     /old_locus_tag="pgaptmp_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HGU4560297.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7948..11169)
                     /gene="carB"
                     /locus_tag="ACHOSM_RS19465"
                     /old_locus_tag="pgaptmp_003893"
     CDS             complement(7948..11169)
                     /gene="carB"
                     /locus_tag="ACHOSM_RS19465"
                     /old_locus_tag="pgaptmp_003893"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGU4560298.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11187..12335)
                     /gene="carA"
                     /locus_tag="ACHOSM_RS19470"
                     /old_locus_tag="pgaptmp_003894"
     CDS             complement(11187..12335)
                     /gene="carA"
                     /locus_tag="ACHOSM_RS19470"
                     /old_locus_tag="pgaptmp_003894"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HGU4560299.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2922 a         3278 c         3340 g         3028 t
ORIGIN      
        1 aataaaacaa acgggaacgc aaaataaata ttcgttttca cagtggaatt aactcatgaa
       61 gaatgaaaag agaaaaacgg gaatagaacc gaaggttttc tttccgccgt taataatcgt
      121 cggcatactt tgttggctta cagtcagaga tctggatgca gcgaatgtcg ttattaatgc
      181 tgtattcagt tacgtcacca atgtatgggg atgggcattt gaatggtata tggtggtgat
      241 gcttttcggt tggttctggc tggtgtttgg cccgtatgcc aaaaagcgtt taggtaacga
      301 accacctgaa tttagtaccg ccagttggat ctttatgatg ttcgcctcct gtacgtctgc
      361 tgccgtactg ttctggggat cgattgagat ctactactac atctccaccc cgccgtttgg
      421 cttagaaccg aactcgacag gggcgaaaga gttggggctg gcttacagct tgttccactg
      481 gggacctctg ccgtgggcca cttacagctt cctttcagtc gccttcgctt acttcttctt
      541 tgtccgcaaa atggaagtga ttcgccccag ctcgacactg gtgccgctgg taggtgaaaa
      601 acacgccaaa gggttgttcg gcactatcgt cgacaacttc tatctcgtcg ccttgatctt
      661 cgcgatgggt accagtctgg gccttgccac gccgctggtg accgagtgta tgcaatggtt
      721 gtttggcatt ccgcataccc tgcaactgga cgctatcatc attacctgct ggattatcct
      781 caacgccatt tgcgtcgcct gcggtctgca aaaaggggta cgtatcgcca gtgacgtgcg
      841 tagttacctg agcttcctga tgctgggttg ggtgttcatt gtcagcggtg ccagcttcat
      901 catgaactac ttcaccgatt cggtggggat gttgctgatg tatctgccgc gcatgttgtt
      961 ctataccgat cccatcgcta aaggcggctt cccgcagggc tggaccgtgt tctactgggc
     1021 atggtgggtg atttatgcta tccagatgag tatcttcctc gcccgcatct cccgtggtcg
     1081 tactgtgcgt gaactgtgct tcggcatggt gctggggctg acagcgtcaa cctggatcct
     1141 gtggactgta ctcggtagta acactctgct gttgatagat aaaaacatta tcaacattcc
     1201 aaatctgatc gaacagtacg gtgtggcgcg cgccattatc gaaacctggg ccgctctgcc
     1261 actcagcacc gccaccatgt ggggcttctt catcctctgc tttattgcca ccgttacgct
     1321 ggttaacgcc tgctcttata ccctggcgat gtccacttgc cgcgaagtac gcgatggtga
     1381 agaaccaccg ctgctggtgc gtatcggttg gtcaattctg gttggcatta tcggtattgt
     1441 tttgctggcg ctcggcggcc tgaaaccgat tcaaactgcc attatcgccg gaggatgccc
     1501 gctgttcttc gtcaacatta tggtgacgct ctcctttatt aaagacgcga aacagaactg
     1561 gaaagattaa ttaaccccca aaatatcaag aggttgaaag atggatttta atttaaatga
     1621 tgagcaggaa ctgtttgtcg ccggtatccg cgaactgatg gccagcgaaa actgggaggc
     1681 ctattttgcc gagtgcgacc gtgacagcgt ctacccggaa cgttttgtca aagcactggc
     1741 ggatatgggt atcgacagtc tgctgatccc tgaagagcac ggtggtctgg acgcggggtt
     1801 tgttactctc gccgccgtgt ggatggagct gggacgtctg ggggcaccaa cctatgtact
     1861 gtaccagttg ccgggcgggt tcaacacctt cctgcgcgaa ggcacacaag agcagatcga
     1921 caagattatg gctttccgcg gcaccggtaa gcagatgtgg aactcagcga ttaccgaacc
     1981 gggcgcgggc tccgacgtgg gtagcctgaa aacgacttat acccgtagaa atggtaagat
     2041 ttatcttaat ggtagtaagt gttttattac cagcagcgcc tacaccccgt acatcgtggt
     2101 gatggcgcgc gacggggctt ctccggacaa acctgtctac accgaatggt ttgttgatat
     2161 gagcaaaccg ggcatcaaag tgaccaaact tgaaaagctc ggtctgcgta tggatagctg
     2221 ctgtgaaatc acctttgacg acgtggaact ggacgagaaa gacatgttcg gtcgggaagg
     2281 taacggcttt aaccgcgtca aagaagagtt cgaccatgaa cgtttcctgg tagccctgac
     2341 caactacggt acggcgatgt gcgcctttga agatgcggcg cgctacgcca accagcgcgt
     2401 gcagtttggc gaggctattg gtcgtttcca gttgattcag gaaaaattcg cccacatggc
     2461 gatcaaatta aactccatga aaaacatgct gtatgaagca gcgtggaaag cagacaacgg
     2521 caccatcacc tctggcgatg cagcgatgtg caaatacttc tgcgccaatg cggcatttga
     2581 agtggtggat agcgcaatgc aggtgctggg cggtgtcggg attgcgggca accaccgcat
     2641 cagccgcttc tggcgtgacc tgcgtgtaga ccgcgtctcc gggggatctg acgaaatgca
     2701 gatcctgacg ctgggtcgtg cggtgctgaa gcaataccgc taagtcaatg cgctgcctga
     2761 tgcgacgctg atgcgtctta tcaggcctac gggagcacga atgtaggccg gataaagcgt
     2821 ttacgccgca tccggcagtc atgcgctaca taaatttttc aggagcaaat aatggatcat
     2881 ctacccatgc cgaaattcgg gccgttggcc ggattgcgcg ttgtcttctc cggtatcgaa
     2941 atcgccggac cgtttgccgg gcaaatgttc gcagaatggg gcgcggaagt tatctggatc
     3001 gagaacgtcg cctgggccga caccattcgc gttcaaccga actacccgca actctcccgc
     3061 cgcaatttgc acgcgctgtc gttaaatatt ttcaaagatg aaggccgcga agcgtttctg
     3121 aaattaatgg aaaccaccga tatcttcatc gaagccagta aaggtccggc ctttgcccgt
     3181 cgtggcatta ccgatgaagt actgtggcag cacaacccga aactggttat cgctcacctg
     3241 tccggttttg gtcagtacgg caccgaggag tacaccaatc ttccggccta taacaccatc
     3301 gcccaggcct tcagtggtta cctgattcag aacggtgatg ttgaccagcc aatgcctgcc
     3361 ttcccgtata ccgccgatta cttttctggc ctgaccgcca ccacggcggc gctggcagca
     3421 ctgcataaag tgcgtgaaac cggtaaaggc gaaagtatcg acatcgccat gtatgaagtg
     3481 atgctgcgta tgggccagta cttcatgatg gattacttca acggcggcga aatgtgcccg
     3541 cgcatgagca aaggtaaaga tccctactac gccggttgcg gtctgtataa atgcgccgac
     3601 ggctacatcg tgatggaact ggtgggcatt acccaaattg aagagtgctt taaagatatt
     3661 ggcctcgcac atctactcgg tacgccggaa atcccggaag gcactcagct tatccaccgt
     3721 atcgaatgcc cttacggccc actggtagaa gaaaaactcg atgcctggct ggcggcacat
     3781 accatcgcag aagttaaaga acgctttgcc gaactgaata tcgcctgtgc caaagtactg
     3841 accgtaccgg aactggaaag caatccacag tatgttgccc gtgaatcaat cactcagtgg
     3901 caaacgatgg atggtcgcac ctgcaaaggg ccgaacatca tgccgaaatt caaaaataac
     3961 cccggacaaa tctggcgcgg aatgccctca catggcatgg acacggctgc cattttgaaa
     4021 aatatcggct acagcgaaaa cgacattcag gagttggtca gcaaaggtct ggccaaagtt
     4081 gaggactaaa cgcgtggcgt cagtcggctt gcctgactag cgcccgccca ataacaaacg
     4141 aacaaaaatg gatagaggtg caatggatat cattggcgga caacatctac gtcaaatgtg
     4201 ggacgatctt gcggacgttt acggtcataa aacggcgctg atttgtgaat ccagcggcgg
     4261 agtcgttaac cggtatagtt atcttgagtt aaatcaggag attaaccgca cggcaaacct
     4321 gttttatacg ctggggattc gcaaaggcga caaggttgca ctacatctcg acaactgccc
     4381 ggaatttatc ttttgctggt tcgggctggc aaaaattggc gcgattatgg tgccgattaa
     4441 cgcccgcctg ttacgcgagg aaagcgcgtg gatcctgcaa aatagccagg cgtgcctgct
     4501 ggtgaccagt gcgcaattct atcctatgta tcaacagatt cagcaggaag atgccactca
     4561 attgcggcac atttgcctga tagatgtggc acttcccgct gatgatggcg tgagttcgtt
     4621 tactcaactg aaaaatcaac aacctgccac cttgtgctat gcaccgccgc tatcgactga
     4681 cgatacggcg gaaattctct tcacctccgg caccacctcc cgaccgaaag gtgtggtgat
     4741 tacccattac aacctgcgct tcgctggata ttactccgcc tggcagtgtg cactgcgtga
     4801 cgatgacgtc tacctgacgg taatgcctgc gtttcatatc gattgccagt gtactgcggc
     4861 gatggcggcg ttttctgccg gggccacctt tgtgctggtc gagaaataca gcgcccgcgc
     4921 cttctgggga caggtacaga agtaccgcgc caccgttacc gaatgtattc cgatgatgat
     4981 ccgtaccttg atggtgcagc cgccttcagc gaacgatcgg caacaccgcc tgcgggaagt
     5041 gatgttttat ctcaacttgt cggagcagga aaaagatgcg ttttgtgaac gcttcggcgt
     5101 tcgcttgctg acgtcttatg ggatgacgga aaccattgtg ggcattatcg gcgatcgccc
     5161 tggcgataaa cgacgctggc cgtcgattgg tagggcgggg ttttgctacg aagcggagat
     5221 ccgcgacgat cacaatcgcc cgctcccggc aggtgagatc ggtgaaatct gtattaaagg
     5281 cgtaccaggg aaaaccatct tcaaagagta ttttctcaac ccgaaagcca ctgcgaaagt
     5341 gctggaagcc gacggctggc tacataccgg cgataccgga tactgcgacg aagagggctt
     5401 tttttatttc gtcgatcgcc gctgcaatat gatcaaacgc ggtggcgaga atgtctcctg
     5461 cgtggagctg gaaaatatca tcgccaccca tccaaaaatt caggatatcg tggttgtggg
     5521 tattaaagat tcgattcgcg atgaagccat caaagcattt gtggtgctga atgaaggtga
     5581 aacattgagc gaagaggaat ttttccgctt ctgcgaacaa aatatggcga aatttaaagt
     5641 gccctcttat ctggagatca gaaaagatct gccacgtaat tgctcgggga aaataattag
     5701 aaagaatctg aaataaatga aacagcaggg aactaccctg ccagctaata atcacgctat
     5761 aaaacaatat gcattttttg cagggatgtt gtcgtccctg aaaaagcaaa aatggagaaa
     5821 aggaatgagt gaatcattac atctgacccg caatggatca attctggaaa ttacccttga
     5881 tcgtccaaaa gcgaatgcta ttgatgcaaa aaccagcttt gaaatgggcg aagtatttct
     5941 aaatttccgt gacgatccgc aattacgtgt cgccattatt accggtgccg gagagaagtt
     6001 cttttccgcg ggctgggatt taaaagcggc agcagaaggc gaagcaccgg atgctgactt
     6061 tggcccgggt ggatttgcgg gattaaccga aattttcaat ctcgacaaac cggttatcgc
     6121 agctgtgaac ggctatgcct ttggcggcgg tttcgaactg gcgctggcgg cagattttat
     6181 tgtttgtgcc gataacgcca gcttcgccct gccggaagcc aagctgggca tcgttcctga
     6241 cagcggcggc gtgctgcgtc taccgaagat cctgccgcct gccatcgtca atgaaatggt
     6301 gatgaccggc agacgaatgg gcgcagaaga ggcgctgcgt tgggggatag tcaaccgcgt
     6361 ggttagccag gcggaactga tggataacgc ccgcgaactg gcgcagcagc tggttaacag
     6421 cgccccgctg gcgattgcgg cgctgaaaga gatcttccgc accaccagcg aaatgccggt
     6481 agaagaagcg tatcgctata ttcgcagcgg cgtgttgaaa cactatccgt cggttctgca
     6541 ttcggaggat gccattgaag ggccgctggc gtttgccgag aagcgcgatc cggtgtggaa
     6601 aggacgttaa caaccgtgag ctattacgcc tttgaggggt taattccggt ggttcacccg
     6661 acggcgtttg tccatcccag tgccgtcttg attggcgatg tgattgtggg agccggtgtc
     6721 tacatcggcc cactcgcctc actgcgtggt gactacgggc ggttgatcgt gcaagcggga
     6781 gccaatattc aggatggctg cattatgcat ggctactgcg acactgacac catcgttggg
     6841 gaaaacggcc atatcgggca cggagcgatc ctgcatggtt gtgtgattgg tcgcgatgca
     6901 ttggtcggga tgaacagcgt gattatggat ggcgcggtca ttggcgaaga gagcattgtt
     6961 gccgccatga gctttgtcaa agcaggcttt cacggcgaga aacgccagtt gttgatgggt
     7021 acgccagccc gcgctgtacg cagtgttagt gacgacgagt tacactggaa acggttgaat
     7081 accaaagagt atcaggatct tgtcgggcgc tgtcatgcat cgttacatga aacacagcca
     7141 ctgaggcaaa tggaggaaaa tcgcccccgt ttgcagggga cgacggatgt gacgcctaaa
     7201 cggtgatctt gccggatgcg acctggttgc tggataagat gctacaacat cgcatctggc
     7261 aagcaggacg cccggtatca acgagataaa attaacgacg catactcttt gacaacatca
     7321 tctgccactt ctgctcgcgg ttaagttccg tgagcggaac ggcaggaata cgcggcgtct
     7381 ttcttacgcc gactaattta tcccggctgt tattgcgcag gcgcgcgtaa atttgctcat
     7441 cgatatcgac cacttttacc agtcgctggc actggcatcc ccgtccttcc agcttattag
     7501 ggatcatctt aacttcgcag cttatttctg cgacttccga cagaatataa gtcagggtat
     7561 taaccgcctt gctgtgttca atatcgaaat gaatagagat ctctcttgct atcacccacc
     7621 gattttcagc catcatccat tcggcgatta acaagtagag tggtttttca acatatcctt
     7681 cacacatacg aactccagtt tcacaaatta aaagtctggc tatgactcgt tttgaagcaa
     7741 ttaaacaagg ccatattaaa attgtggata tatcgatagt atgcaacttc accgtcgata
     7801 agtgtgagct aaatcctgct tatgtcatca aaaatatcga ttccccaaaa gatttactta
     7861 atgggcaaaa aaaacggtcc tcatcagaga accgtattac ttattcgatc aaattagcgg
     7921 atgaaaaata tccgccatga cacgctatta tttgatctgc gcgtgcattt cctgcaccga
     7981 aattactttt tcggtcgcgt cggcattcag cgccatcgcg gtagcgaaac caccgttcag
     8041 ggtggtgtca taatgcactt tatattgcag cgcactgcga cggatcacgc gggagtcttc
     8101 aatcgcgcga cggcctgaag tggtgttgat gatgtaggta tattcgccat tcttgatgcg
     8161 gtcctgaatg tgcggacggc cttcatgcac cttgttcacc agacgcggat taatgcccgc
     8221 ttcgcccagc acaatcgccg tgccgtgggt cgcatccagc tcgaagccct gtttcagcag
     8281 ttttgccgcc aggtccacca cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg
     8341 accgtgtttc ttcatggtgg agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa
     8401 ggtgcggccc acgcccatca cttccccggt ggagcgcatt tctggcccta acagcgggtc
     8461 aacacccggg aatttattga acggcagcac cacttctttc accgagtagt acggcgggat
     8521 aacttctttg gttacgccct gctcagccag cgatttgcca gccatcacac gcgccgccac
     8581 ttttgccagc ggtacgccgg tggctttgga gacgaacgga acggtacgcg ccgcacgcgg
     8641 gttaacttca atcaggtaga cttcgttgtt tttcaccgca aactgcacgt tcatcagacc
     8701 gcgcacctgc aattcgaagg ccagtttctg cacctgctgg cgcatcacat cctgaatttc
     8761 ctgacttaag gtgtaggctg gcagagaaca tgcggagtca ccggagtgca cgcccgcctg
     8821 ctcgatgtgc tccatgatgc cgccaatcag caccatttcg ccgtcgcaga tggcatccac
     8881 gtcaacttct accgcgtcat caaggaagtg atccagcaac actggcgcat cgttagacac
     8941 gctgaccgcc gtctggaagt agcgacgcag gtcagcttcg tcatagacga tttccatcgc
     9001 ccgaccgccg agaacgtaag acggacgtac caccagcggg tagccaatct ctttcgcctt
     9061 ctctaccgcc atttcaatag cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg
     9121 ctcaaccgca tgctggaagc gttcacggtc ttctgcacgg tcgatagcat ccgggctggt
     9181 gccgataacc ggtacgccag cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc
     9241 gccgtactga acgataacgc ctttcggctt ctcaatacgc acgatttcca gcacatcttc
     9301 cagagttacc ggctcgaagt agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc
     9361 cgggttacag ttaaccataa tggtttcgta accgtcttcg cgcagcgcca gcgaggcgtg
     9421 tacacagcag tagtcgaatt cgataccctg accgatacgg ttcgggccgc cgccgagaac
     9481 catgattttt tcacggtcgg tagacggatt cgcttcgcac tcttcttcat aagtggagta
     9541 catgtaagcg gtgtcggtgg cgaactctgc cgcacaggta tccacgcgct tataaaccgg
     9601 gtgcaggtca tactggtcgc gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt
     9661 tgccagacgc gcatcggcaa agcctttgcg tttcagctgg cgcaggaatt cagcgttcag
     9721 gccagtgatg cccacttccg ctactttctc ttccagacgc accagttctt caatctgtac
     9781 caggaaccag cggtcaatat tggtcaggtt gaacacgccg tccacagaca ggcccgcacg
     9841 gaaggcatcg gcgatgtacc agatacgctc tgcgccagca tctttcagtt cgcgacggat
     9901 tttggttaac gcttccgggt catcgaggct cactttcggg tcgaatccag tcgcaccgac
     9961 ttccaggccg cgcagcgctt tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac
    10021 ttcgccaacc gatttcatct gagtggtcag acggtcgtta gcaccagcga atttttcgaa
    10081 gttgaagcga ggaattttag taaccacata gtcgatggac ggctcgaagg aggccggagt
    10141 acgtccgcca gtgatgtcgt tcatcagttc gtcgagggtg taacccaccg ccagtttcgc
    10201 cgccacttta gcaatcggga aaccggtcgc tttcgacgcc agcgccgaag aacgggacac
    10261 gcgtgggttc atttcgataa caatcaggcg accgtttttc gggttcaccg caaactggac
    10321 attggaacca ccggtttcaa cgccgatttc acgcagcacc gccatcgagg cgttacgcat
    10381 gatttgatat tctttgtcgg tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt
    10441 atggatgccc atcgcatcaa agttttcgat agagcagacg atgatgcagt tgtcgttttt
    10501 atcacgcacc acttccatct cgtactcttt ccagccgatc agcgactcat caatcagcag
    10561 ctctttggtt ggggagagat ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt
    10621 ataagcgata ccgccgccgc taccgcccat ggtaaaggat gggcgaataa tgcacgggaa
    10681 gcccacgtca gcggcaaccg ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc
    10741 ggtttccaga ccaattttct tcatcgctac gtcgaaacga cggcggtctt ctgctttatc
    10801 aatcgcatcg gcggtcgcac caatcatagt cacgccgaac tcttccaaca cgccctgacg
    10861 ctccagctcc agcgcgcagt tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc
    10921 cgggcgctct ttttcaataa tcttgcgtac tacttcccag tgaatcggct cgatgtaggt
    10981 cgcatcggcc atttccgggt cggtcatgat ggtcgccggg ttggagttca ccagaatgac
    11041 gcggtaaccc tcttcgcgca gggctttaca tgcttgcgcg ccagagtagt caaactcaca
    11101 cgcctgaccg ataacaatcg ggcccgcacc cagaatcagg atacttttta tatctgtacg
    11161 ttttggcatg gctcttttac tcctgattac ttagcggttt tacggtactg ctcaattaac
    11221 tcgataaagt ggtcgaacaa cggcgctgcg tcgtgtggcc cagggctggc ttccgggtga
    11281 ccctggaagc tgaacgccgg tttatcagtg cgatgaatgc cctgtaacgt accgtcgaac
    11341 agggatttat gcgtgacacg caggtttgca ggtaatgttg cttcatccac cgcaaaaccg
    11401 tggttctggg cggtgatcat caccacgttt ttctccacat ctttaaccgg atggttgccg
    11461 ccgtggtgac caaatttcat tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga
    11521 tgaccgaggc agatgccgaa taccggaata tcggtttcga ggaatttctg gatggcggta
    11581 atggcgtaat cgcacggggc cgggtcgcca ggaccgttgg agaggaagat gccgtctgga
    11641 ttcattttca gcacatcttc cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct
    11701 ctatccacca gcatccgcag gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg
    11761 aacggcagct cgtcttcttt tttcgcttct ggcaggccgc cggtcaacgt ccagctccct
    11821 tgtgtccagc tataggcttc tgcggtggtc acttcttttg ccagatccat gccattcaga
    11881 cctgggaacg cgcgggcttt ttctaacgcc aacgccgcat ccgggttatc gcctgcgata
    11941 atgcagccat tctgtgcgcc tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata
    12001 tcggcaatcg ccacgatgtt atggcgcttc aggtaagaag agaggtcttc ggtattacgg
    12061 aagttgctgg caatcagcgg caggtcgcga atcaccagac cttgtgcatg tacctgagaa
    12121 gattcttcat cggcgtcatt agtgccgaca ttgccaatat ggggataagt aagagtaacg
    12181 atttgacgag aataggaagg atcagtgagg atttcttgat aaccggtcat tgaagtattg
    12241 aaaacgactt ccccaaccgc cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt
    12301 ccgtcttcca gaaccaatag cgctgactta atcaaaacac cctccagaga atattcactc
    12361 actttatttg catattaatt cacaatgatg acgtaaatca atgcaaatct gcttaccgac
    12421 gaatttctgg caaacggcgg cattctggag atatgggcgc taaaagtcaa cttaatggcg
    12481 ggatttttta tattattttg tggctcatgc atgttttaca ggcattaagc gacaaaaaga
    12541 tcatataacc tgttctggaa aacgcttg
//
LOCUS       DAZKHC020000112        12311 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZKHC020000112 DAZKHC020000000
VERSION     DAZKHC020000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.112"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..395
                     /locus_tag="ACHOSM_RS19475"
                     /old_locus_tag="pgaptmp_003895"
     CDS             <1..395
                     /locus_tag="ACHOSM_RS19475"
                     /old_locus_tag="pgaptmp_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGU4560300.1"
                     /translation="KQSKNLNFLNVSGKNNCPTMQLMLFNLFSPALKLNTGLAILSPG
                     AFEVHSDGIDVDNELFHYPIKKAYTPYNIHTYKTEEVVNQRNIKVKNMTLDEINNSYC
                     NNDYYNQAIREEPIDLLDRSFSSSSWPF"
     gene            719..2107
                     /gene="narU"
                     /locus_tag="ACHOSM_RS19480"
                     /old_locus_tag="pgaptmp_003896"
     CDS             719..2107
                     /gene="narU"
                     /locus_tag="ACHOSM_RS19480"
                     /old_locus_tag="pgaptmp_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HGU4560301.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2189..5929
                     /gene="narZ"
                     /locus_tag="ACHOSM_RS19485"
                     /old_locus_tag="pgaptmp_003897"
     CDS             2189..5929
                     /gene="narZ"
                     /locus_tag="ACHOSM_RS19485"
                     /old_locus_tag="pgaptmp_003897"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HGU4560302.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPSEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHHKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5926..7470
                     /gene="narH"
                     /locus_tag="ACHOSM_RS19490"
                     /old_locus_tag="pgaptmp_003898"
     CDS             5926..7470
                     /gene="narH"
                     /locus_tag="ACHOSM_RS19490"
                     /old_locus_tag="pgaptmp_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU4560303.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7470..8165
                     /gene="narW"
                     /locus_tag="ACHOSM_RS19495"
                     /old_locus_tag="pgaptmp_003899"
     CDS             7470..8165
                     /gene="narW"
                     /locus_tag="ACHOSM_RS19495"
                     /old_locus_tag="pgaptmp_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU4560304.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGEK"
     gene            8162..8842
                     /gene="narI"
                     /locus_tag="ACHOSM_RS19500"
                     /old_locus_tag="pgaptmp_003900"
     CDS             8162..8842
                     /gene="narI"
                     /locus_tag="ACHOSM_RS19500"
                     /old_locus_tag="pgaptmp_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU4560305.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGVAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLHGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8922..9815
                     /locus_tag="ACHOSM_RS19505"
                     /old_locus_tag="pgaptmp_003901"
     CDS             8922..9815
                     /locus_tag="ACHOSM_RS19505"
                     /old_locus_tag="pgaptmp_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HGU4560306.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9911..10756)
                     /gene="nhoA"
                     /locus_tag="ACHOSM_RS19510"
                     /old_locus_tag="pgaptmp_003902"
     CDS             complement(9911..10756)
                     /gene="nhoA"
                     /locus_tag="ACHOSM_RS19510"
                     /old_locus_tag="pgaptmp_003902"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HGU4560307.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVTARRGGYCFEQNGVLERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVV
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10931..11500
                     /locus_tag="ACHOSM_RS19515"
                     /old_locus_tag="pgaptmp_003903"
     CDS             10931..11500
                     /locus_tag="ACHOSM_RS19515"
                     /old_locus_tag="pgaptmp_003903"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HGU4560308.1"
                     /translation="MSRFIPVELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDGKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11504..11731)
                     /gene="pptA"
                     /locus_tag="ACHOSM_RS19520"
                     /old_locus_tag="pgaptmp_003904"
     CDS             complement(11504..11731)
                     /gene="pptA"
                     /locus_tag="ACHOSM_RS19520"
                     /old_locus_tag="pgaptmp_003904"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HGU4560309.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(11831..>12311)
                     /locus_tag="ACHOSM_RS19525"
                     /old_locus_tag="pgaptmp_003905"
     CDS             complement(11831..>12311)
                     /locus_tag="ACHOSM_RS19525"
                     /old_locus_tag="pgaptmp_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGU4560310.1"
                     /translation="HDCSAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVA
                     VSFRSIIAEQKKEPEMMVRYYISSADLTAEKFATAIRNHWHVENNLHWRLDVVMNEDD
                     CKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMGRNYLASVLTGSGLS"
BASE COUNT         2945 a         3176 c         3171 g         3019 t
ORIGIN      
        1 taaaacagag caaaaactta aattttttaa atgtttctgg gaagaacaat tgccctacta
       61 tgcagctcat gttatttaat ttgttttccc cagcacttaa gcttaatact ggcctggcaa
      121 ttctttcgcc tggtgcattt gaagttcact ctgacggaat agatgtggat aacgaattgt
      181 ttcactatcc tattaaaaaa gcatataccc catataatat acacacttac aagacagagg
      241 aagttgtaaa ccagaggaat ataaaagtta aaaacatgac cttagatgaa ataaacaata
      301 gttactgtaa taacgattat tacaatcagg caataagaga ggaaccgata gaccttctgg
      361 acagatcgtt ttcctccagt tcatggcctt tttagtgaat aaatcatcac taattctata
      421 agtgtctggt cttgttgata atatagttaa tatgtaaccc tttagattca cgatctgaag
      481 ggttgaaaca tgacatacgt tcaaagatta acccccgttt gaaggttagc ttgttagtta
      541 catttagtaa cacatatctg gctacttaat ccccattctg cccccgcggt tacgctttct
      601 tatctattct ttcaatcagc acttcaccca attcatcctt aattctaata actggctatt
      661 gcgccttatc tcctgttttg tcttttttca ttaccaatgt gtgcatgtga ggaacaatat
      721 ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc gactggaaac cagaaaatcc
      781 ggccttctgg gaaaataaag gaaaacatat tgctcgaaga aacctctgga tatcagtcag
      841 ttgtctactt cttgccttct gtgtctggat gctatttagc gcagttaccg ttaatctcaa
      901 taaaatcggt tttaatttta ctaccgatca actcttttta ttaaccgcat taccctccgt
      961 ttctggcgca ttattgcgtg ttccctactc ctttatggtg cctatattcg gtggacgccg
     1021 atggacggtt tttagtactg caatcctgat tattccttgc gtctggctcg gaattgccgt
     1081 gcaaaatccg aatactcctt ttgggatatt tatcgttatc gctttgctat gcggttttgc
     1141 aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc ttctttccaa aagccaaaca
     1201 agggagcgct cttgggatta atggcggatt aggaaactta ggtgtaagtg taatgcagct
     1261 ggttgcaccg ctggtcattt ttgtacctgt atttgccttt ctcggcgtca atggcgtacc
     1321 gcaggccgac ggttcggtga tgtcgctggc gaatgccgca tggatttggg taccgctact
     1381 ggcgattgcc acgatcgccg cctggtcagg gatgaatgat atcgccagtt cacgcgcctc
     1441 aattgccgac cagctccctg tcttacaacg cctgcatctc tggctgctga gcctgcttta
     1501 ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt tttgccatgc tggcaaaaac
     1561 ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt ggcccattta tcggtgccat
     1621 cgcgcggtcg gttggtggtg ctatttccga taagttcggc ggcgtgcggg tgacgttgat
     1681 caactttatt tttatggcga ttttcagtgc cctgctgttc cttaccttac cgggcacagg
     1741 ctccggtaat ttcatcgcct tttacgccgt atttatgggg ctgtttctga ctgcgggtct
     1801 gggaagtggt tctactttcc agatgatcgc cgtcatcttt cgccagataa ccatttatcg
     1861 ggtaaagatg aaaggcggta gtgatgagca agctcagaaa gaagccgtca ccgaaacggc
     1921 ggcggctctg ggctttatct cagccattgg cgcagtgggc ggctttttta ttccgcaggc
     1981 gtttggcatg tcgctcaata tgaccggctc tccggtcggc gcgatgaaag tgtttttaat
     2041 cttctacatc gtttgtgtgc tgctgacctg gctggtttat ggtcggcgga agttcagcca
     2101 aaaataagag atcgatgcct gatgtacaac cgtgtcgcat ccgacaaggc atcagatccc
     2161 aattcattcc tggagcagga gtcatgtcat gagtaaactt ttggatcgct ttcgctactt
     2221 caaacaaaag ggcgaaacct ttgccgatgg tcacggacag gtgatgcata gcaaccgcga
     2281 ctgggaggac agctatcgcc agcgttggca gttcgacaaa atcgtgcgtt ccacccacgg
     2341 tgtaaactgt acaggctcct gtagctggaa aatctacgtt aaaaatggtc tggtgacctg
     2401 ggaaatccaa cagaccgact acccgcgcac tcgccctgac ctgcccaatc atgaacctcg
     2461 cggctgcccg cgtggcgcaa gttactcctg gtatctttac agcgctaacc gcctgaaata
     2521 cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa gccctcaagc aacacagcga
     2581 tccggtactg gcgtgggcat cgattatgaa cgatccgcaa aagtgcctga gctacaaaca
     2641 agtgcgtggg cgcggcgggt ttatccgctc caactggcag gaactaaacc agctgattgc
     2701 cgccgctaac gtctggacca tcaaaaccta cggcccggat cgcgttgccg gtttctcgcc
     2761 gatcccggcg atgtcgatgg tttcttacgc cgccggaacg cgttatctgt cactgcttgg
     2821 cggcacctgt ttaagcttct acgactggta ttgcgacctg ccgcccgcct cgccgatgac
     2881 ctggggcgag caaaccgacg taccggaatc tgccgactgg tataactcca gctacatcat
     2941 cgcctggggg tctaacgtac cgcagacacg tacgccggac gcccacttct ttaccgaagt
     3001 acgctacaaa ggcactaaaa ccatcgccat tacccctgac tactctgaag tggccaaatt
     3061 gtgcgaccag tggctggcac cgaaacaagg cactgatagc gccctggcga tggcaatggg
     3121 ccatgtgatt ttaaaagagt ttcatctcga taatcccagc gactacttta tcaactactg
     3181 ccgccgctac agcgacatgc cgatgctagt aatgctggag cctcgcgacg atggtagcta
     3241 cgttcccggg cgcatgatcc gcgcatctga cctggtggat ggactgggcg aaagcaacaa
     3301 tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa ttggtagtgc cgaacggttc
     3361 gattggtttc cgctggggag aaaaaggcaa atggaatctg gaatccattg ccgccggtac
     3421 ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac gctgttgcag gcgtggcctt
     3481 cccctacttt ggcggcattg aaaatccgca ttttcgcagc gtaaaacaca atccggtgct
     3541 ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat ggcaacacct gtccggtggt
     3601 cagcgtttat gatttggtac tggcgaatta cggcctcgat cgcgggctgg aagatgaaaa
     3661 cagtgcgaaa gattacgctg aaatcaaacc gtacacccca gcctggggtg agcaaattac
     3721 cggcgtgccg cgccagtata ttgaaaccat cgcccgtgaa tttgccgata ccgcccataa
     3781 aacgcatggg cgctcgatga ttatcctcgg cgcaggtgtt aaccactggt atcacatgga
     3841 catgaactac cgcgggatga tcaatatgct gatcttctgc ggttgtgtcg ggcaaagcgg
     3901 tggcggctgg gcacactatg tcggccagga aaaactgcgc ccacaaaccg gctggttgcc
     3961 actggccttt gcgctcgact ggaaccgacc accgcgccaa atgaacagca cctcgttttt
     4021 ctacaatcat tccagccaat ggcgctatga aaaagtctct gctcaggagt tactttcacc
     4081 gctcgccgat gccagtaagt acagcggtca tctgattgat ttcaacgttc gcgccgaacg
     4141 tatgggctgg ctaccttctg cgccgcagct ggggcgtaac ccgctcggga ttaaagctga
     4201 agccgacaag gccggattat cccccacaga atttaccgcc caggcgctga aatcgggcga
     4261 tttacgtatg gcctgcgaac aaccagatag cggcagcaat catccgcgta atttgtttgt
     4321 ctggcgttct aacctgcttg gctcctccgg caaaggccac gagtatatgc agaagtatct
     4381 gctggggacc gaaagcggga ttcagggcga ggaactcggt gccagcgacg ggatcaaacc
     4441 ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag ctcgacctgc tggtgacgct
     4501 cgacttccgc atgtccagta cctgcctgtt ctccgatatc gttctgccca ccgccacctg
     4561 gtacgaaaaa gacgatatga atacctcgga tatgcatccg tttattcatc cgctttctgc
     4621 ggcggtcgat ccggcgtggg aatcacgcag cgactgggaa atctacaaag gtatcgccaa
     4681 agcattttcg caagtgtgcg tgggtcatct tggcaaagaa accgacgtgg tattacaacc
     4741 cctactgcat gactctccgg cagagctctc acagccgagt gaagtgctcg actggcgcaa
     4801 aggcgaatgc gatctgattc caggcaaaac cgcgccgaat attgtggcgg tggagcgcga
     4861 ctaccctgct acgtatgaac gctttacctc gctcgggcca ttgatggaca aacttggcaa
     4921 tggcggtaaa gggatttcgt ggaataccca ggatgaaatc gatttcctcg gcaaactcaa
     4981 ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg attgacaccg ccattgacgc
     5041 ttcagaagtg attctggcac tggcaccgga aaccaacggt catgttgcag tcaaagcgtg
     5101 gcaggcgctg ggcgagatca ccgggcgcga acatacccat ctggcgctgc acaaagagga
     5161 cgagaagatt cgctttcgcg atattcaggc gcagccgcgt aaaattatct ccagccccac
     5221 ctggtccggt ctggaaagcg atcacgtatc ctataacgcg ggatacacca acgttcatga
     5281 gttaattccg tggcgcacgc tgtcgggacg ccagcagctc tatcaggatc atccgtggat
     5341 gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct atcgacaccc gtagcgtcag
     5401 tgagatgcgc cagataccgc caaacggctt cccggaaaaa gcacttaact tcctgacgcc
     5461 gcaccataaa tggggcattc actcaaccta cagtgaaaac ctgctaatgc tgacgctctc
     5521 tcgcggtgga ccgattgtct ggatcagcga aacagatgcc cgagaactga ccattgtcga
     5581 taacgactgg gtggaagtat tcaacgccaa cggcgcgctg actgcccgcg cggtggtcag
     5641 ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc caggaacgca ttatgaatat
     5701 tcctggttcg gaagtaactg gcatgcgcgg cggcattcat aactcggtta cccgcgtttg
     5761 cccgaaacca acgcatatga ttggcggtta cgcgcagctg gcctggggct ttaactacta
     5821 cggcaccgtc ggctcgaacc gcgacgaatt catcatgatc cgcaaaatga aaaacgttaa
     5881 ctggctggat gatgaaggtc gcgatcaggt acaggaggcg aaaaaatgaa aatacgttcg
     5941 caagtcggca tggtgcttaa tctcgataaa tgtatcggct gccatacctg ttcggtgacc
     6001 tgcaaaaacg tctggaccgg tcgcgaaggc atggagtacg catggtttaa caacgtcgaa
     6061 accaaaccgg gcattggtta tccgaaaaac tgggaagatc aggaagagtg gcaaggcggc
     6121 tgggtccgcg atgtgaatgg caagatacgc ccgcgtctgg gcagcaagat gggtgtaata
     6181 accaaaatct tcgccaaccc ggtggtgccg cagattgatg attactacga acctttcacc
     6241 ttcgactacg aacatttgca tagcgcaccg gaaggcaaac atatccctac tgctcgcccg
     6301 cgttcgctga ttgacggcaa gcggatggac aaagtgatct gggggccaaa ctgggaagaa
     6361 ctgctgggcg gcgagtttga aaaacgtgcc cgcgaccgca acttcgaggc catgcaaaag
     6421 gagatgtacg ggcagtttga aaacaccttc atgatgtacc tgccgcgcct gtgcgaacac
     6481 tgcctcaatc ccagttgcgt ggcgacctgc ccaagcggcg ctatctacaa acgcgaagaa
     6541 gacggcattg tgctgattga tcaggataaa tgccgtggct ggcgtttgtg cattagcggt
     6601 tgtccgtaca aaaaaatcta cttcaactgg aaaagcggca agtcagaaaa atgtatcttc
     6661 tgttatccgc gaattgagtc aggccaacca accgtatgct cagaaacctg cgtgggtcgc
     6721 atccggtatc tgggcgtgct gctttacgac gccgaccgca ttgaggaagc ggcaagcacc
     6781 gagcgcgaag ttgacctcta tgagcgccag tgcgaagtgt tcctcgatcc acacgatccc
     6841 tctgtgatcg aggaagccct gaaacaaggt attccacaaa acgtgattga agctgcccag
     6901 cgttcgcctg tctacaaaat ggcgatggac tggaaactgg cgctaccgct gcaccctgaa
     6961 tatcgcaccc tgccgatggt ctggtacgtt cctccgctgt cgccgattca gtcctacgca
     7021 gatgcgggcg gtttgccgaa aagcgaaggc gtgctgcccg ccatcgaaag cctgcgtatt
     7081 ccggtgcaat atctcgccaa tatgttgagt gccggcgata ccggtccggt actgcgggcg
     7141 ctgaaacgga tgatggcgat gcgccactat atgcgttcac aaaccgtgga aggcgttact
     7201 gatactcgtg ccatcgacga agtaggcctg agcgtcgccc aggtcgaaga gatgtatcgt
     7261 tacctcgcca ttgccaacta tgaagatcgt tttgtcatcc cgacgagcca tcgggaaatg
     7321 gcgggcgatg ccttcgcaga acgcaacggc tgcggtttta cctttggcga cggttgtcac
     7381 ggctcggaca gcaaattcaa cctgttcaac agtagccgta tcgatgccat caacatcacc
     7441 gaagtgcgcg acaaagcgga gggcgaataa tgcagatcct caaagtgatc ggcctgttga
     7501 tggagtatcc ggacgagctg ttgtgggaat gcaaggagga cgcgctggcg ttgatccgcc
     7561 gcgacgcgcc gatgcttacg gatttcaccc acaacctgct taacgcgccg ctgctggata
     7621 aacaggccga atggtgcgaa gtgtttgacc gcgggcgcac cacgtcgctg ctgctgttcg
     7681 aacatgttca tgccgagtcc cgcgatcgcg gccaggcaat ggtggacctg ctggcggagt
     7741 atgaaaaggt cggcctgcaa ctggattgtc gggaactgcc cgattatcta ccgctgtatc
     7801 tggagtattt aagcgtactg cctgacgatc aggcgaaaga aggattgctc aatgttgcgc
     7861 cgatcctcgc cctgcttggc ggtcgcttaa aacaacgcga ggcaccgtgg tacgcgctgt
     7921 ttgatgctct gctgcaactg gcgggaagct ccctttcaag tgacagtgtc acaaaacaga
     7981 ttcgcagcga agagcgcgat gacacccgtc aggcgctgga tgcagtgtgg gaagaggaac
     8041 aggtgaagtt tattgaagat aacgccacgg cgtgtgacag ctcgccgctt aatcaatatc
     8101 agcgacgctt tagccaggat gtcgcgccgc agtatgtcga catcagtgcg ggaggtgaga
     8161 aatgattcag tatctgaacg tcttttttta cgatatctac ccgtacattt gtgcgacggt
     8221 gtttttcctc ggcagctggc tgcgctacga ctacgggcag tacacctggc gcgcctcctc
     8281 aagtcaaatg ctcgataaac gcgggatggt tatatggtcg aatctgttcc atatcggcat
     8341 tttggggatc ttcttcgggc acctgttcgg catgttaacg ccgcactgga tgtacgcgtg
     8401 gtttttgcca gtggcagcga aacaactaat ggcgatggtg ctcggcggta tttgcggtgt
     8461 gttgacattg attggcgtcg caggactgct gtggcgcagg ctgacaaatc agcgcgtgcg
     8521 tgcaacttcc actacgccgg atattatcat catgagcatt ttgctgatcc agtgtctgct
     8581 ggggctaagt accataccgt tttcagcaca gtatcctgat ggtagcgaaa tgatgaagct
     8641 ggtgggttgg gcgcaaagca ttgtcacttt ccgtggtgga tcgtcagaaa tgcttcacgg
     8701 cgtagcgttc gttttccgcc tgcatctggt attgggaatg accatcttcc tgctcttccc
     8761 gttcaccaga ttggtgcatg tgtggagcgc gccgtttgag tactttactc gtcggtatca
     8821 gattgtgcgc tcgcggcgat aattttcacc ctggccggat agcccttcgc tctccggcca
     8881 cttgcaggct atgatagttc tccagtcaca ggagaacgtt aatgaaaccg caggtttacc
     8941 acgtcgatgc ttttacctct caaccatttc gcggcaattc tgccggtgtt gttttccccg
     9001 ccgataatct tagcgaagcg caaatgcagc ttatcgcccg cgaattaggt cattcggaaa
     9061 ccgcttttct gctgcacagc gacgacagcg atgtgcgcat ccgctacttt acgccaacag
     9121 ttgaagtgcc gatttgcggt cacgcgacag tagctgcgca ctatgtacgt gcgaaggtgt
     9181 taggtttagg aaattgcacg gtctggcaaa catcactggc aggaaaacat cgcgtgacta
     9241 tcgaaaagca caacgatgat tatcgtattt cgctggaaca aggtacgccg ggctttgagc
     9301 caccgctgga aggtgaaaca cgtgaggcga ttatcaacgc gcttcatctc actgaagacg
     9361 atattttgcc gggcttgcca attcaggtcg caacaacagg acactcaaaa gtgatgatcc
     9421 cgttgaaacc ggaagtggat atcgacgccc tttcgcctga tcttgctgcg ctgagcgcta
     9481 tcagcaaaca gattggctgc aatggtttct tcccgttcca gatccgccca ggcaaaaacg
     9541 aaaccgatgg tcgtatgttc tcgcctgcga ttggtattgt ggaagatccg gtgactggca
     9601 atgccaatgg cccgatgggt gcatggttgg tacatcacaa cgtattgccc cacgatggca
     9661 acgtgttgtg tgttaaaggc catcaggggc gcgcattggg gcgtgacggc atgattgaag
     9721 tgacggtgac aattcgtgat aaccaaccgg aaaaagtcac catttcaggc acagcggtga
     9781 ttttgtttca tgccgaatgg gcaattgaac tctgaaaccg ttgcctgatg cgcttcgcct
     9841 gtcaggtcta caaccgagct aatgtatgcc ggataaggcg tttacgccgc atccggcaac
     9901 ctgacataaa ttattttccc gcctccgggt gcgtatcaaa cgccgccatc accagcgcta
     9961 actcatccac ggtaaagcca tgtttcgcat catccacgcc cagaccaaac tgttcttgca
    10021 tcacagcata taatgacgcc acatccggta gatttcgctg ctccaccgcg tgcccatttt
    10081 cataatgggt aaaatgaaaa ttggtcagcg ttagcttgcc accgtccggc aaatggcggc
    10141 acatcagcaa atgatggcga aaatgcgact gcggccagtg cgccgaccag aaattgccca
    10201 tcacataatc gctttgttgc tgctcgcaga gatcaaaacg gtacatcgac tgccaatgct
    10261 gatgatgatt aaactgcacc acccaatcat caccctcctg caacaaccga tactctccgt
    10321 gtggcgtggt ctgcacgaga tcggaaacta aacgaatcgg cgcggttagc gtctgcccac
    10381 cgaaaccgac atcagcaatc catttttcct cttccagttc caccaacagc aaacgatggg
    10441 tgcgcggcgg taatgctggc ggatttgata acactacgcg ccctaacaag ctgcgaacgt
    10501 taaaccccag ctcgcgtaac acccgctcta acacgccatt ctgctcaaaa cagtaaccgc
    10561 cacgccgggc ggtcaccagt ttctcttcca gcgattgatc atcaagctgt atttccctcg
    10621 gcagcaaaac gtcgaggttt tcaaacggaa tggtgcaatt gtgtttcagg tgcaatgcac
    10681 gaagcgtatc aatattgacc gcagcagctc ccgaccagtt aatacgggca aaatagtgat
    10741 tcagaatggg cgtcatcagg cgtttccctt agtggttttc tcaacttata acctggaata
    10801 gacgccgcac tggctatttt gtgcgatcgc ggacatgcat cacgttcgca aatcgccaat
    10861 cccgttaatt tcgtcgctta ttgcgttacg aactacgctt ttaaaacaac cttcttagcg
    10921 aggcgatacc gtgagccgat tcatccccgt agaattacac catgccagcc gtctgttaaa
    10981 tcatggccca actgtcttga tcaccagttt cgatgagcaa tcccagcggc gtaacattat
    11041 ggctgcagcc tggtcaatgc cggtggagtt tgaaccgcca cgcgtggcga ttgtggtaga
    11101 taaatcgaca tggaccaggg agttgattga acgtaacggt aaatttggca tcgttatccc
    11161 gggcgttgcg gcgactaact ggacgtgggc ggtgggaagc gtgtcgggtc gtgaagaaga
    11221 taaatttaat tgctacggca ttccggtagt gaaaggcccg gtgcttggtt tgcctctggt
    11281 cgaggaaaaa tgtctggcgt ggatggagtg tcgcctgcta cctgcgactt ctgcgcaaga
    11341 aaaatacgac acactgtttg gcgaggtagt atcagcagcg gcagacgcac gggtatttgt
    11401 cgaaggccgc tggcagtttg atgatggtaa gctcaatacg ttgcatcatt taggtgctgg
    11461 cacgtttgtt actagcggca agcgagttac ggcgggttaa gcattacatg ctgtaaccgg
    11521 gtttctttat caaagcctcc atttggggct cgatttcggc atcccagata gcttgccaag
    11581 attctggttg aatctgctgt agagcaatgc ttatcgagct atctttactg ttcagatggc
    11641 gaataataac gtcggtaata tctgcagcaa gtgctgcttt ttgttgttcg tccagttcac
    11701 gcggaaagca tttaatgtcg atgtgcggca tgattacgct ccttttgaaa gatgtcagta
    11761 aaattaacac acattgtgaa tgtcatgtgt ccggtaaagc gtttatacga caataatgcc
    11821 cagggcaaga ttacgaaagc ccgctccccg taaggactga cgccaggtag tttctgccca
    11881 tggctgcttt tcgcatctta cgtcttaacc ctgccttgaa taccttatca ttcgtcaaaa
    11941 tattaatagc aatgtgccgt atccctgaaa ataattctgc tgcatttcct cttcttattt
    12001 tgcagtcgtc ttcattcatt accacgtcca gacgccagtg cagattattc tccacgtgcc
    12061 agtgatttcg aatcgctgtc gcaaacttct cagcggttaa atcagcagaa ctgatataat
    12121 atctgaccat catttcgggc tctttctttt gctctgctat tattgagcga aaggagactg
    12181 ccacgcataa ttttttcagc cctttccatt caaacgtgaa atcaataagt tcatcaggga
    12241 catcgcaaac aatatgaaga cggatttctt ctctgccgtg actcttttca ctaattgcgg
    12301 aacagtcatg c
//
LOCUS       DAZKHC020000113        12045 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZKHC020000113 DAZKHC020000000
VERSION     DAZKHC020000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.113"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(153..1652)
                     /gene="feaB"
                     /locus_tag="ACHOSM_RS19530"
                     /old_locus_tag="pgaptmp_003906"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(153..1652)
                     /gene="feaB"
                     /locus_tag="ACHOSM_RS19530"
                     /old_locus_tag="pgaptmp_003906"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HGU4560311.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNSVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADRLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWSETKSVCVRY"
     gene            1888..2793
                     /gene="feaR"
                     /locus_tag="ACHOSM_RS19535"
                     /old_locus_tag="pgaptmp_003907"
     CDS             1888..2793
                     /gene="feaR"
                     /locus_tag="ACHOSM_RS19535"
                     /old_locus_tag="pgaptmp_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HGU4560312.1"
                     /translation="MNPAMDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(2965..3294)
                     /gene="ydbL"
                     /locus_tag="ACHOSM_RS19540"
                     /old_locus_tag="pgaptmp_003908"
     CDS             complement(2965..3294)
                     /gene="ydbL"
                     /locus_tag="ACHOSM_RS19540"
                     /old_locus_tag="pgaptmp_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HGU4560313.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLIA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(3299..3484)
                     /gene="ynbE"
                     /locus_tag="ACHOSM_RS19545"
                     /old_locus_tag="pgaptmp_003909"
     CDS             complement(3299..3484)
                     /gene="ynbE"
                     /locus_tag="ACHOSM_RS19545"
                     /old_locus_tag="pgaptmp_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HGU4560314.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(3481..6120)
                     /gene="ydbH"
                     /locus_tag="ACHOSM_RS19550"
                     /old_locus_tag="pgaptmp_003910"
     CDS             complement(3481..6120)
                     /gene="ydbH"
                     /locus_tag="ACHOSM_RS19550"
                     /old_locus_tag="pgaptmp_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HGU4560315.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            6328..7317
                     /gene="ldhA"
                     /locus_tag="ACHOSM_RS19555"
                     /old_locus_tag="pgaptmp_003911"
     CDS             6328..7317
                     /gene="ldhA"
                     /locus_tag="ACHOSM_RS19555"
                     /old_locus_tag="pgaptmp_003911"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU4560316.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            7428..7850
                     /gene="hslJ"
                     /locus_tag="ACHOSM_RS19560"
                     /old_locus_tag="pgaptmp_003912"
     CDS             7428..7850
                     /gene="hslJ"
                     /locus_tag="ACHOSM_RS19560"
                     /old_locus_tag="pgaptmp_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HGU4560317.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(7847..8113)
                     /gene="ydbJ"
                     /locus_tag="ACHOSM_RS19565"
                     /old_locus_tag="pgaptmp_003913"
     CDS             complement(7847..8113)
                     /gene="ydbJ"
                     /locus_tag="ACHOSM_RS19565"
                     /old_locus_tag="pgaptmp_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HGU4560318.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            8387..11911
                     /gene="nifJ"
                     /locus_tag="ACHOSM_RS19570"
                     /old_locus_tag="pgaptmp_003914"
     CDS             8387..11911
                     /gene="nifJ"
                     /locus_tag="ACHOSM_RS19570"
                     /old_locus_tag="pgaptmp_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HGU4560319.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DSYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLIRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPSDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2998 a         3168 c         2927 g         2952 t
ORIGIN      
        1 gattatgccg accgaatggg tacatactct gctgaaacca tggaacttct ttgacgaaac
       61 gccaacgcta ggggcgctga agaaagataa ttgattgttt cagactaaaa aaacggcacc
      121 aggtgccgtt ttttacttat gagcgaacaa gattaatacc gtacacacac cgacttagtt
      181 tcactccaac cgtccagcca atccgggcca aaatcacggc ctgttcctga ctgcttcatc
      241 ccaccaaacg gtaagttagc gtcaattaag gtatgactgt ttacccacac cgtccctgcc
      301 tgtaagcgat cgctatattc cagagcctgg gagagatttt gcgtccagac gctggcagtt
      361 aagccatatt ccgtgtcgtt tgccagttgt aacgcctctt ctccatccgc tactcgcacc
      421 aggttaacca ccggaccaaa cacctcttca cgagttaagc gcaatttagc atcgggattt
      481 accaccagcg ttggcgcaac ataatacccc tctccggctg gtccattcga tccgcgaatc
      541 agctctgctt gctgtgcctg cgcatcgtcg aggaatgaac acactttgtc gcagtgcgca
      601 cgagaaacca aagggttaat ctgtgcaaca ggtgacatcc ccggtcccac ttgcaacgat
      661 tttaccgcct gctcaaatcc actaaccagc gtgtcaaaca acggcgcttc aatataaatt
      721 cgcgaactgg cggcgcatac ttgcccttga ttcaggaagc ttccggtcat caagccttca
      781 ataacccatt gcggatcagc atcttttaat acaattgccg ggtttttacc gcccagttcc
      841 agcgttacac gcgttaagcg atcagcagca gttctggcaa taccttttcc cgtcgcggtt
      901 gaaccggtaa aactgatttt cgcaacatga ggatgtgacg tcagggccgc gccgcataca
      961 gcacctgacc cggtgacgac attaaaaacg ccatcaggga taccagcctc actggccagt
     1021 tccgccacgc gcaacatcgt cagtggcgtg gtttccgaag gcttaatcac gattgaacag
     1081 cctgctgcca gtgctggcat caccttccac atgccaatca tcaacggaaa gttccatggc
     1141 acaattcccg ctaccacgcc aaccggctct ttacgcgtcc aggcctgata acgcgccccc
     1201 tggggtaagg gaatcgacaa gtccagcgtt ttacccgcga ttttggtcgt taacccggcg
     1261 gtataacgca tccagttcag cgtacagccc acttcaaaag cacgggaaat ggcaattgac
     1321 ttgccttgct ccagggtttc cagttgcgcc agctcctcac tgtgctgctc caccagatca
     1381 gcaaaacgta gcagaatacg ttcacgctct gcgggtaatc gcccggccca gcgacgcgag
     1441 acaaaggccc gccaggcaga catgactgag ttatctacat ccgcttcgtt ggcatcagca
     1501 gtagacgcaa tttcttgccc ggtggccgga tcaaagatcg ccaaccgttt ttcactttgt
     1561 gcggggccag gacgaccatc aatataaaga ccgtgttgac gatcgagaaa ctgttggacc
     1621 tggcttaata ctgctacatg cggctctgtc atcacttttc cttattattt acccagtgtg
     1681 atttcagtgt acgcagcgac aagaaaagtc acttttttcg ctgtgacatt cgctttgtca
     1741 gctgtgacaa gctccgcaaa tcgtgacaat aaccgcttat gtctctatgc ctgacaagac
     1801 ttatttccta tagtcaggct cattagcttc gtttattgca acacaaatgc aacaataaaa
     1861 atacatttca cagagcgaaa acgtgccatg aaccccgcaa tggataatga gtttcagcaa
     1921 tggctttccc aaatcaatca ggtatgcgga aattttaccg gacgcctgct gactgagcgt
     1981 tacacgggtg tactggacac gcattttgcc aaaggactaa agctgagtac cgtgacaacc
     2041 agcggggtga atttatcccg cacctggcag gaggtaaaag gcagcgacga tgcctggttt
     2101 tacaccgttt ttcagcttag tggtcaggca ataatggagc aggatgagcg tcaggtgcag
     2161 attggcgctg gcgatattac gttactcgat gcctcacgcc cctgttcgct ttactggcag
     2221 gagtcttcta aacagatttc attacttttg ccacgcactc tgctggaaca atattttccc
     2281 catcaaaaac ctgtctgcgc agaaagactg gacgctgact tacccatggt gcaactcagt
     2341 catcgcctgt tacaggagag catgaataat ccggcacttt ctgaaacaga aagtgaagct
     2401 gcgctacagg cgatggtgtg tctgctgcgc ccggtacttc atcagcggga atctgttcaa
     2461 cctcgtcgtg aacgtcagtt tcaaaaagtg gttacgttga tagacgataa tattcgcgaa
     2521 gagatattac gcccggagtg gatagccgga gagacaggta tgtcagtacg tagtttgtac
     2581 cgaatgtttg ccgataaagg tttggtggtc gcgcaatata ttcgtaaccg tcgtctcgat
     2641 ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa aactggcagg catcggcttt
     2701 cattggggat tttctgacca gagtcatttt tcaacggtat ttaagcaacg ctttgggatg
     2761 acgccaggcg agtatcgacg taaattccgc taactaattg gcgttgtgtg tactgcaacg
     2821 ccaattagtt aaaacagata tttgactatg catttttcag ggcgaaaggc aatttgacag
     2881 gagtttttga atattcacct tgtttcagga aaatagatca atcgctttct cctgtagcgt
     2941 tatcccgata ttgcggtttt gctattaaaa ttttcgcacc catttaccgt ttatcccttg
     3001 tacatattcc cccggtttgg cccgagctac gagcttttgc ccggcgagtt tcgcgatatc
     3061 atctacagac acattatttt gctttgccag ttgttggtaa ctcgctttac gttcggcatt
     3121 aatgtcggtt actaatttct ctgtttcagc atccgttttc agcgcaatca gataaccata
     3181 aaatgtttcc ccgacccgcc cctgcgttct ggcttcatcc agagtcaatg ccattacatt
     3241 gctgcttacc agcccaacaa gaaacgcaca aagaagtaat gttttcttca tcatcgcctc
     3301 agaaaagatc gctacgagtt tcaagcagct cttcgacatc tttgtctgcc ttgatgatga
     3361 tctcatgctc aattttaacg ttcatattga tagtgatcgg ttccttgggc gcagcgactt
     3421 caatacgcgg cgtacaggca gccagcataa aagatgacgt caacgcagcc agtaaaattt
     3481 tcattgtttt tcctcacact cttttccttg cggacagtca tttcccggca gacgtgcgtt
     3541 ctgctccaac catgcctgga gattatcgcc aaagcgtaaa ctgcgccaca gcgtaaaaat
     3601 attctcttca tgatggtaat taaggtttac cgtaccgctt ttaccatcaa cgcgactggt
     3661 acctgtaatg ttggcctgca tggttaataa accgagatta tctaaattaa tatttgtcga
     3721 tgaacggcta atttccatat agcgcaacca gttaattgct gaacccgcag tcatattgtc
     3781 tttcaccacc gcatctgcgg tgtctttatc cagccgcaag gtcattggcc cgctatttgc
     3841 cagccaacca tctttcacta tccatttttc gttgtttagc cataacggca atgcaccact
     3901 aaatgccccg gacatggcga attgtttcgg attgacggcg ctaaccagtt cgctggatga
     3961 gagattattc agccgcaaca gcgccgggtc atgttgcggc atacgtaatt gtttcatcag
     4021 tacgttaccg cctaacacat cgacgctaac atcagtcaac agcaagggtt cttcttcagt
     4081 ccacggataa cgcccttgca aatcagccgt aatatttttc gctgtcacca gattaatcac
     4141 ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga tgccaggctc catcggcaaa
     4201 acggaaaggc aggacaaaat cgacaccgtt aacctgatta tctggcatcc aggcactacc
     4261 gcctttcaac acgccgtgcc ctcccgcgcg gaatccttgt tcaggcgcag cagaaaatgc
     4321 aacctgagca tacagttcac catcgcgtaa gttcatcttc cagtcgggtg gcaccagcgg
     4381 ctggaataca gtcagtgatt gttttggcca ccaggcgtta ccgcgcagac gaataccatc
     4441 ccagcgacca tttacccgaa ccgggccaat ctcaccagca tgtaaatcgc ctttaaagag
     4501 gaaatacgtc ggatcgcgcc catcgacgct aaattttaag gttgatggcg gtaacacact
     4561 gccgccagtg aacagcgttt gcccggcgtc cagtgacagc gcgccgctga aggagggatg
     4621 ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt aatcgcggct tttctaccgt
     4681 catcgtaccg tattgtaact ggtcgaaacc ggtggagagg tcggtcagcg taatcgtgct
     4741 gtcatgccat tcaccttttc ctgcgacatc ccaggtggca ttcatcggtg taaaactgcc
     4801 ttttccccag tagcgccact gccagcggcc cgcgtcaggg agaaaatcat tcgccagccc
     4861 atccatatgc agcacgaaat cgcccagttc attttcatgc gcctgcaaaa tggcctgcaa
     4921 acgtccgtca acaccacgtt gggtgacttt tacacccgct aaaggccagc ggatttcatc
     4981 gatgtccagc gaatcgatga ctcttccctt cgaacgtaat aacgcgcctg gctcaaaggc
     5041 cagcgttggg tcagtcagac ttccacttag ctgcgcgggt aagcgggcat ataaaatgag
     5101 gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc tggctgttat ccatgcttaa
     5161 ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc gcttgcccct gggtcagcac
     5221 actcagtcgt ccgctgatca gagcgttctc aagccctgcc tgccagttgt cgactttgac
     5281 acccagtcgg ccactcaaag gaaaacctgc atacggccaa ctccagcgac catcgcttac
     5341 ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc aacggatcgc cgttatcccg
     5401 tgccagcaca atcaattgcc cgctattttc ctgccagtcc agctctgcat ccaccagtga
     5461 cggttcctgc ggcaagttta acgtcgcagt agcatgccca cttacaggaa gtccatccgg
     5521 cacgagcggc atagtaaatt cccccaccag tttaaccggc ggctgatttt caaacgcgac
     5581 gacatccagt tcgctgactg taagttgttg ccctttcagc tggccttgaa atttaacttt
     5641 ttcgccctga taacgcagtt gctggatatc agaggttaat gcgagagaga gttttccctg
     5701 ccattcctgc cagggagaaa aaatcagttt atcgatattg atccaggtgt taggcagcat
     5761 ggactgccac tgcgcgaggg tttttggagc agctggcgat tgctccgtct gcggcaattt
     5821 cgccagacaa gcagaatcaa gttctaccgt gccgacgttc aataaccagc ggctgggatg
     5881 tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct cccaccagat aacggagatc
     5941 gggaatgatt aaacctttac gcgtaatgcg tgggctttca tctaatgcaa tacgtgtccc
     6001 gagcggtagc cagatgcccg ccagcgtggg aacccacagc ccgagcgtca tcaacagcgt
     6061 caacggcaca agaataatca gtaataacag cgcgagaacg gctttatatt tacccagcat
     6121 gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc aatacgggta ttgtggcatg
     6181 tttaaccgtt cagttgaagg ttgcgcctac actaagcata gttgttgatg aatttttcaa
     6241 tatcgccata gctttcaatt aaatttgaaa ttttgtaaaa tatttttagt ggcttaaatg
     6301 tgattcaaca tcactggaga aagtcttatg aaactcgccg tttatagcac aaaacagtac
     6361 gacaagaagt acctgcaaca ggtgaacgag tcctttggct ttgagctgga attttttgac
     6421 tttctgctga cggaaaaaac cgctaaaact gccaatggct gcgaagcggt atgtattttc
     6481 gtaaacgatg acggcagccg cccggtgctg gaagagctga aaaagcacgg cgttaaatat
     6541 atcgccctgc gctgtgccgg tttcaataac gtcgaccttg acgcggcaaa agaactgggg
     6601 ctgaaagtag tccgtgttcc agcctatgat ccagaggccg ttgctgaaca cgccatcggt
     6661 atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc agcgtacccg tgacgctaac
     6721 ttctctctgg aaggtctgac cggctttact atgtatggca aaacggcagg cgttatcggt
     6781 accggtaaaa tcggtgtggc gatgctgcgc attctgaaag gttttggtat gcgtctgctg
     6841 gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg gtgtggagta tgtcgatctg
     6901 ccaaccctgt tctctgaatc agacgttatc tctctgcact gcccgctgac accggaaaac
     6961 taccatctgt tgaacgaagc cgccttcgat cagatgaaaa atggcgtgat gatcgtcaat
     7021 accagtcgcg gtgcattgat tgattctcag gcagcaattg aagcgctgaa aaatcagaaa
     7081 attggttcgt tgggtatgga cgtgtatgag aacgaacgcg atctgttctt tgaagataaa
     7141 tccaacgacg tgatccagga tgacgtattc cgtcgcctgt ctgcctgcca caacgtgctg
     7201 tttaccgggc accaggcatt cctgacagca gaagctctga ccagtatttc tcagactacg
     7261 ctgcaaaact taagcaatct ggaaaaaggt gaaacctgcc cgaacgaact ggtttaatct
     7321 tgccgctccc ctgcaatcca ggggagctga ttcagataat ccccaatgac ctttcattct
     7381 ctattcttaa aatagtcgtg agtcagaaac tgtaattgag aaccacaatg aagaaagtag
     7441 ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt aagtaatgac aaaattgctg
     7501 ttacgccaga acagctacag catcatcgct ttgtgctgga aagcgtaaac ggtaagcccg
     7561 tgaccagcga taaaaatccg ccagaaatca gctttggtga aaaaatgatg atttccggca
     7621 gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa tggtgaactg acagccaaag
     7681 ggctggcaat gacccgtatg atgtgcgcta acccgcagct taatgaactc gataacacca
     7741 ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac cgcgaaccag ttaacgctgg
     7801 cgaccgcgaa acagacatta acttataagc tggcggattt aatgaattaa tagctgccac
     7861 agctcccggc ggcaagtgac tgttcgctac agcgtttgcc gttgggtaat gcacacatcc
     7921 caatcgccgt accatccagt tgacgggcaa cagaaagcga accgccgatc attgcacaat
     7981 ttgcttctcc actactggac atcgacgctt ttaaacctgg cgctacgtgc gccgcagtcg
     8041 cctgctgaac aggttcacta ctacacgccg acaacaataa agcggcacac cctacccaaa
     8101 acgctgctcg catcttttct tcctctgatc ttcaagccaa acgacaccgc cataaataat
     8161 aggcagcaca gagggcggcg tcgagagctg tcctgcgcgt tgccccgcat ttttactttt
     8221 ttatggctat ttttttgccc tctgtttgat caaaacattc attacgctga tgtgggggac
     8281 acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt gaaggattac tactgggcgc
     8341 gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg tgtcatatga ttactattga
     8401 cggtaatggc gcggttgctt cggtggcgtt tcgcaccagt gaagttatcg ccatctaccc
     8461 tattaccccc agttccacga tggcagaaca ggctgatgcc tgggccggaa acggcttgaa
     8521 gaacgtttgg ggagacacac cacgcgtggt tgaaatgcag tcggaagcgg gtgctatcgc
     8581 taccgtgcat ggcgctttgc agacgggtgc cctttcaaca tcgtttacgt catcgcaggg
     8641 tttgctgctg atgatcccga cgctgtacaa actggcaggc gaactcacac cgtttgtcct
     8701 gcatgtagcg gcacgtaccg ttgccacaca tgcactctct atttttggcg atcattccga
     8761 cgttatggcg gtgcgccaga cgggttgcgc gatgttgtgt gcggcaaacg tccaggaagc
     8821 gcaagacttt gctctcattt cgcacatcgc gacgctgaaa agccgcgtgc catttattca
     8881 tttctttgat ggtttccgta cgtcccacga aatcaataaa attgtcccgc tggccgatga
     8941 cactattctt gatctcatgc cgcaggccga aattgatgct catcgcgccc gggcgctcaa
     9001 cccggaacat ccggtgatcc gcggtacgtc cgccaatcct gactcttatt tccagtctcg
     9061 cgaagccacc aacccatggt acaacgcggt ctatgaccat gttgaacagg cgatgaatga
     9121 tttctctgcc gcgacaggtc gtcagtatca gccgtttgaa tattacgggc atccgcaagc
     9181 ggaacgggtg attatcctga tgggctcagc cattggcacc tgtgaagaag tggttgatga
     9241 attgctaatc cgtggcgaaa aagtcggcgt gctgaaagtt cgcctgtacc gccccttctc
     9301 cgccaaacac ttgctgcaag ctctgccggg atccgtacgc agcgtggcgg tactggacag
     9361 aaccaaagaa cccggtgccc aggcagaacc gctctatctg gatgtgatga ccgcactggc
     9421 agaagccttt aataatggtg agcgcgaaac cctgccccgt gtcattggtg ggcgctatgg
     9481 tctttcatcc aaagaatttg gcccggactg tgtactggcg gtatttgccg agctcaacgc
     9541 ggctaaacct aaagcacgct ttacggttgg tatttacgat gatgtgacca atctgtcact
     9601 gccgttgccg gaaaacaccc tgccaaactc ggcgaaactg gaagccttgt tttatggcct
     9661 tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc aagattatcg gtaattccac
     9721 gccgtggtac gcacagggct attttgttta cgactccaaa aaggcgggcg gcctgacggt
     9781 ttctcacctt cgcgtgagcg aacagccgat tcgttccgct tatctcattt cccaggctga
     9841 ttttgttggc tgccaccagt tgcagtttat cgataaatat cagatggctg agcgtttaaa
     9901 acctggcggc attttcctgc tcaacacgcc gtacagcgca gatgaagtgt ggtcgcgctt
     9961 gccgcaggaa gttcaggccg tgctaaatca gaaaaaagcg cgcttctatg tgattaacgc
    10021 ggcgaaaatc gcccgcgaat gtggcctggc ggcccgtatt aataccgtca tgcagatggc
    10081 ttttttccat ctgacacaaa ttctgcctag cgatagcgcc ctcgcagaat tgcagggtgc
    10141 gattgccaaa agttacagta gcaaaggtca ggatctggtg gaacgcaact ggcaggctct
    10201 ggcgctggcg cgtgaatccg tagaagaagt tccgttgcag ccggttaatc cgcacagcgc
    10261 caatcgaccg ccagtggttt ccgatgccgc tcctgatttc gtgaaaaccg taaccgctgc
    10321 gatgctcgcc gggcttggtg atgccctccc cgtttcggcg ctgccgccag acggcacctg
    10381 gccgatgggc accactcgct gggaaaaacg caatatcgcc gaagagatcc ccatctggaa
    10441 agaggaactc tgtacccaat gtaaccactg cgtcgccgct tgcccacact cagctattcg
    10501 cgcaaaagtg gtaccgcctg aagcgatgga aaacgcccct gccagcctgc attcgctgga
    10561 tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg caggtggctc cggaagattg
    10621 caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa gaccgtcaga atccagagat
    10681 taaagccatc aatatgatgt cgcgcctgga acatgtcgaa gaagagaaaa tcaattacga
    10741 tttcttcctc aacctgccag aaatcgaccg tagcaaactg gaacgtattg atattcgtac
    10801 atcgcagctg attacaccgc tgtttgaata ttcaggtgct tgctccggtt gtggcgagac
    10861 gccgtatatt aaattactga ctcagctcta tggcgaccgg atgttgatcg ctaacgccac
    10921 cggctgttct tcaatttatg gcggtaacct gccctctaca ccgtatacca ccgatgccaa
    10981 cggtcgtggg ccggcatggg cgaactctct atttgaagat aatgccgaat ttggccttgg
    11041 tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg cgtctgctgg atcaatttgc
    11101 cgataaaatc ccggcggaat tactgacggc gttgaaatca gacgctacgc cagaggttcg
    11161 tcgagaacag gtcgcagctt tacgccagca actcaacgat gttgccgaag cacatgaact
    11221 gctacgtgat gcagatgcac tggtggaaaa atcaatctgg ctgattggtg gtgatggctg
    11281 ggcttacgat atcggctttg gcggtctgga tcatgtattg agtttgacgg aaaacgtcaa
    11341 cattctggtg ctggatacgc aatgctattc caacaccggt ggtcaggcat cgaaagcgac
    11401 accgctgggt gcagtaacta agttcggcga gcacggcaaa cgtaaagcgc gtaaagatct
    11461 tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg cagatttctc tcggcgcgca
    11521 gctcaaccag acggtgaaag cgattcagga agcggaagcg tatccggggc catcgctaat
    11581 catcgcttat agcccatgtg aagagcatgg ttacgatctg gcactcagcc acgaccagat
    11641 gcgccaactc acagctaccg gcttctggcc cctatatcgc ttcgatcctc gtcgtgccga
    11701 tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg tcagaagcac tggaagaaac
    11761 gttacttcac gagcaacgtt tccgtcggct gaattcgcag cagccagaag tggcagaaca
    11821 gttatggaaa gatgctgcag ctgatttgca aaaacgctat gacttcctgg cacaaatggc
    11881 cggaaaagcg gaaaaaagca acaccgatta aatgctctgg ataaggatta tccaattcta
    11941 aaaaaaaagc ccggacgact gttcgggctt gtctttttat atgttggaaa atcagtggca
    12001 atgcaatggc ccaacagaaa ataaaatctg tgacaataaa ggcat
//
LOCUS       DAZKHC020000114        11894 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZKHC020000114 DAZKHC020000000
VERSION     DAZKHC020000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.114"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            96..965
                     /locus_tag="ACHOSM_RS19575"
                     /old_locus_tag="pgaptmp_003915"
     CDS             96..965
                     /locus_tag="ACHOSM_RS19575"
                     /old_locus_tag="pgaptmp_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HGU4560320.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLADVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1168..1521)
                     /locus_tag="ACHOSM_RS19580"
                     /old_locus_tag="pgaptmp_003916"
     CDS             complement(1168..1521)
                     /locus_tag="ACHOSM_RS19580"
                     /old_locus_tag="pgaptmp_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HGU4560321.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1659..3305)
                     /gene="groL"
                     /locus_tag="ACHOSM_RS19585"
                     /old_locus_tag="pgaptmp_003917"
     CDS             complement(1659..3305)
                     /gene="groL"
                     /locus_tag="ACHOSM_RS19585"
                     /old_locus_tag="pgaptmp_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGU4560322.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3349..3642)
                     /locus_tag="ACHOSM_RS19590"
                     /old_locus_tag="pgaptmp_003918"
     CDS             complement(3349..3642)
                     /locus_tag="ACHOSM_RS19590"
                     /old_locus_tag="pgaptmp_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGU4560323.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3918..5174
                     /gene="yjeH"
                     /locus_tag="ACHOSM_RS19595"
                     /old_locus_tag="pgaptmp_003919"
     CDS             3918..5174
                     /gene="yjeH"
                     /locus_tag="ACHOSM_RS19595"
                     /old_locus_tag="pgaptmp_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HGU4560324.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5190..5666)
                     /locus_tag="ACHOSM_RS19600"
                     /old_locus_tag="pgaptmp_003920"
     CDS             complement(5190..5666)
                     /locus_tag="ACHOSM_RS19600"
                     /old_locus_tag="pgaptmp_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HGU4560325.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            6003..7439
                     /gene="aspA"
                     /locus_tag="ACHOSM_RS19605"
                     /old_locus_tag="pgaptmp_003921"
     CDS             6003..7439
                     /gene="aspA"
                     /locus_tag="ACHOSM_RS19605"
                     /old_locus_tag="pgaptmp_003921"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HGU4560326.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7557..8858
                     /gene="dcuA"
                     /locus_tag="ACHOSM_RS19610"
                     /old_locus_tag="pgaptmp_003922"
     CDS             7557..8858
                     /gene="dcuA"
                     /locus_tag="ACHOSM_RS19610"
                     /old_locus_tag="pgaptmp_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HGU4560327.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8974..9312
                     /gene="cutA"
                     /locus_tag="ACHOSM_RS19615"
                     /old_locus_tag="pgaptmp_003923"
     CDS             8974..9312
                     /gene="cutA"
                     /locus_tag="ACHOSM_RS19615"
                     /old_locus_tag="pgaptmp_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HGU4560328.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9288..10985
                     /gene="dsbD"
                     /locus_tag="ACHOSM_RS19620"
                     /old_locus_tag="pgaptmp_003924"
     CDS             9288..10985
                     /gene="dsbD"
                     /locus_tag="ACHOSM_RS19620"
                     /old_locus_tag="pgaptmp_003924"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HGU4560329.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSSPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11022..11597
                     /locus_tag="ACHOSM_RS19625"
                     /old_locus_tag="pgaptmp_003925"
     CDS             11022..11597
                     /locus_tag="ACHOSM_RS19625"
                     /old_locus_tag="pgaptmp_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4560330.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELHRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRLGGALT"
     gene            11704..11779
                     /locus_tag="ACHOSM_RS19630"
                     /old_locus_tag="pgaptmp_003926"
     tRNA            11704..11779
                     /locus_tag="ACHOSM_RS19630"
                     /old_locus_tag="pgaptmp_003926"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11737..11739,aa:Phe,seq:gaa)
BASE COUNT         2859 a         3079 c         2922 g         3034 t
ORIGIN      
        1 tatgttattt taacaactat tactaccaag cccatttatc tggcttataa attaaaatta
       61 attatttaat tgagaaatgg ttagggagaa cctacatggc cataagcatc aagggtgtta
      121 ataccggtgt tattcgcaag agtaataatt tcattgcact ggcgctgaaa attaaagaac
      181 ctcgcaataa agaatcgttg ttctttatgt ctgtcatgga actgcgcgac ctgctgattg
      241 cgctggaaag tcgtatgcat caaaagcata aactggatgc tgctgcacgc cttcagtatg
      301 agcaagctcg cgataaagtc attaagaaaa tggcagaaaa catcccagaa attctggttg
      361 atgagcttaa aaatgccgat attaatcgtc gggttaacac gctggaactt accgataatc
      421 aaggcgaaaa tcttaccttc gtattaacgc tacatgacgg tagcaaatgt gagttagtcg
      481 tcaatgagtt gcagattgaa atgctggcgc gagcaatcat tcatgcaatt aataatgcgg
      541 aaatgcgtga actggcatta cgtattacct cactgttaga tttcctgccg ttatatgacg
      601 ttgactgcca ggaaaatggc aatctggaat atgacaccta ttcccaaccg gaatggaaac
      661 ataacctgtt cgaccattat ctggcggtgc tttaccgctt taaagatgaa agtggcaaag
      721 aacaatttag cggcgcagtg gtgaaaacgc gtgaagcaac gccgggtaaa gaaatagagg
      781 cgatcactcg ccgaatgctt gatttcagcc cacgactgaa aaaactcgcg gatgtgccat
      841 gtcaggtcta tgttcgcacc gtagcggcga ataacgccca accgctaacc caggatcaat
      901 gcttacgcgc gctgcatcac ttacgtgttc aatccaccag caaaactgca ccacaggcga
      961 aataatctca tctttgctaa tgcgaggctg gagccatcca gcctgaaatg actgataacg
     1021 tgatcgttgg tcatgccgga tgcggcgtaa acgccttatc cggcctacaa attctcaagc
     1081 attcaatcag ttgcaatgcc tgctgtaggc ctgataagcg aagcgcatca ggcaataata
     1141 cgtttgtcac ccagcctctg gatcacatca gttcgggcac ttataaacct gaccgataat
     1201 ctggctatcc gtcgggacaa aactggacaa cattccctgc gacgggctgc tgatgccata
     1261 aatcacgtta ccgcccattg cagccgcctg gttgcgcaga tcgtttgctg cgccgcgcat
     1321 agaaccgccc tcttctccgt gttgcccgga aagccagttg ctttgcttac ctgtcgcagt
     1381 accaatcagc tggcactctg cgcctggctg ctcgtccaca atgcgtacac tctgaccggc
     1441 agcactcaat tcgttgctgg agctacaacc cgccatcagt agcgcggcac cgacaatccc
     1501 tgctaagtat tttacgtgca tgttattccc cataatcaat gagctggacg ccactcgcgt
     1561 cgtccgtgtc tgaatcttat actagaaaga tgaccaaaag aaaaaccccc agacatttct
     1621 gcccgggggt ttgtttattt ctgcgaggtg cagggcaatt acatcatgcc gcccatgcca
     1681 cccatgccgc ccataccgcc agcagcgcct aagtcagctg catcgttttt cggcaggtcg
     1741 gtaaccatgc actcggtggt gatcatcagg ccagccacag aagccgcgta ctgcagagca
     1801 gaacgggtta ctttggttgg atccaagata cccatgtcga tcatgttgcc gtattcttcg
     1861 gttgctgcgt tgtaaccgta gttgccgtcg ccgcctttaa cggtgttagc aacaacagac
     1921 ggttcttcgc cgcagttcag gacgatctga cgcagcggag cttccattgc acgcagtgca
     1981 actttgatac ccacgttctg gtcttcgttc tgaccacgca ggtcagccag tttagacgct
     2041 acgcggatca gcgcaacacc accaccagca accacgcctt cttctaccgc agcacgggtc
     2101 gcgtgcaggg cgtcttcaac gcgtgctttt ttctctttca tttcaacttc ggtagcagca
     2161 cctactttga taactgcaac gccgcctgcc agtttcgcta cgcgttcctg cagtttttca
     2221 cggtcgtagt cagaagttgc ttcttcaatc tgctgacgga tctgagcaac acggccctgg
     2281 attgcagctt cttcacccac gccatcgatg atagtggtgg tgtctttgtt gatcacaaca
     2341 cgtttagcct gacccaggtc ttcaagggtt gctttttcca gttccatacc gatctcttca
     2401 gagatcacgg taccgccagt cagggttgcg atatcctgca gcatagcttt acgacgatcg
     2461 ccgaagcccg gagctttaac tgcagcaact ttcacgatgc cacgcatggt gttaacaacc
     2521 agagttgcca gcgcttcgcc ttctacatct tcagcgatga tcagcagcgg tttgcctgct
     2581 ttggcaacgg cttccagaac cggcagcatt tcgcggatgt tggagatttt cttgtcagcc
     2641 agcaggatga acgggctttc cagttctact gcgccagttt ccggcttgtt gatgaagtaa
     2701 ggagacaggt agccacggtc gaactgcata ccttcaacca cgtccagttc gtcctgcaga
     2761 ccggtaccgt cttcaacggt gataacgcct tctttaccga ctttgtccat cgcttcagcg
     2821 atcagtttac ctacggtttc gtcggagtta gcggagatgg taccaacctg agcaatcgct
     2881 ttagagtcag agcacggtac ggacagcgct ttcagttctt caactgcagc ggtaacggct
     2941 ttgtcgatac cacgtttcag gtccatcggg ttcatgcccg cagcaacagc tttcagaccc
     3001 tcagtgatga tagcctgagc cagtacagtt gcagtggtgg taccgtcgcc tgcagcgtcg
     3061 ttcgctttag aggcaacttc tttcaccatc tgcgcaccca tgttttcgaa cttgtcttcc
     3121 agttcgattt cacgagcaac ggaaacacca tctttggtga tggtcggtgc accgaaagat
     3181 ttatccagaa ctacgttacg gcctttcgga ccgagggtaa ctttcactgc atctgccagt
     3241 acgtttacgc cgcgcagcat tttcacacga gcgtcgttac cgaattttac gtctttagct
     3301 gccattatct ttattcctta aattcgtatg ttcagtgtcg tgcgaggatt acgcttcaac
     3361 aattgccaga atgtcgcttt cggacatgat caacacttct tcattgtcga tcttctcaga
     3421 tttcacaccg tagccatcgt tgaaaataac gatgtcgcca actttcacat ccagcggctt
     3481 cacttcgcca ttttcaagga tacggccatt gccgacagcc agcacttcgc cgcgggtgga
     3541 tttagccgct gcagagccgg tcagaacgat gccgccagca gatttagttt caacttcttt
     3601 acgcttgacg atcacgcgat catgcaatgg acgaatattc attgataact ctcctttgag
     3661 aaagtccgta tctgttatgg gtgacgccgg agcttacgtg gtttcccggc tggtgaccag
     3721 agaaatgggg atgaggattc gccccttcaa gggggaaaaa gaaaaaaaat tctgatcagc
     3781 acaaaatcgg gtgaaaaccc tgattcacct cacatttcat cgcaatttct tcatcgccgt
     3841 cataagcgaa tctgattgtg ctaccatcga aaatctacgc agttgcccaa aatttgggcg
     3901 caatcggata tcaacccatg agtggactca aacaagaact ggggctggcc cagggcattg
     3961 gcctgctatc gacgtcatta ttaggcaccg gcgtgtttgc cgttcctgcg ttagctgcac
     4021 aggtagcagg caataacagc ctgtgggcgt ggcccgtttt gattatctta gtgttcccga
     4081 ttgcgattgt gtttgcgatt ctgggtcgcc actatcccag cgcaggcggt gtcgcgcact
     4141 tcgtcggtat ggcgtttggt tcgcggcttg agcgagtcac cggctggttg tttttatcgg
     4201 tcattcccgt gggtttgcct gccgcgctac aaattgccgc cgggttcggc caggcaatgt
     4261 ttggctggca tagctggcaa ctgttgttgg cagaactcgg tacgctggcg ctggtgtggt
     4321 atatcggtac tcgcggtgcc agttccagtg ctaatctaca aaccgttatt gccggactta
     4381 tcgtcgcgct gattgtcgct atctggtggg cgggcgatat caaacctgcg aatatcccct
     4441 tcccggcacc tggtaatatc gaacttaccg ggttatttgc tgcgttatca gtgatgttct
     4501 ggtgttttgt cggtctggaa gcatttgccc atcttgcctc ggaatttaaa aatccagagc
     4561 gtgattttcc tcgcgcattg atgattggtc tgctgctggc aggattagtc tactggggct
     4621 gtacggtagt cgtcttacac ttcgacgcct atggtgaaaa aatggcggcg gcagcatcgc
     4681 ttccaaaaat tgtagtgcaa ctgttcggtg taggagcgtt atggattgcc tgcgtgattg
     4741 gctatctggc ctgctttgcc agtctcaaca tttatataca gagcttcgcc cgcctggtct
     4801 ggtcgcaggc gcaacataat cctgaccact acctggcacg cctctcttct cgccatatcc
     4861 cgaataatgc cctcaatgcg gtgctcggct gctgtgtggt gagcactttg gtgattcatg
     4921 ctttagagat caatctggac gctcttatta tttatgccaa tggcatcttt attatgattt
     4981 atctgttatg catgctggca ggctgtaaat tattgcaagg acgttatcga ctactggcgg
     5041 tggttggcgg gctgttatgc gttctgttac tggcaatggt cggctggaaa agtctctatg
     5101 cgctgatcat gctggcgggg ttatggctgt ttctgccaaa acgaaaaacg ccggaaaatg
     5161 gcataaccac ataatccggc gtttcgacat taatcctggc gatcgtcttt atgatcaagg
     5221 cggtcgcgct catcatcctt tcgctggtac tcaccatcaa aagtattacc gccaccggtc
     5281 ccggcgctaa aaccgccgcc tggcatacga gaaaagcgca aatgcggcat caacttcact
     5341 gtcagatgct tttgcaccgg cggtaataaa agcagaagac cgaggaagtc ggtgaaaaag
     5401 cccggtaata aaagcagcaa accggcaatg atcagcgaaa cacttttaat catctccgcc
     5461 gctgggtttt cacccgccgc cattttttgc tgcatcagca cgaaattctt aaagccctgg
     5521 ttacgtacca gtgacatccc gataactgac gtgaatataa ccagcacgag ggtcagcaat
     5581 acccccaata catgggcaac ctgaataaag attgaaatct caatatagac ataaaggaaa
     5641 atggcaataa aaggtaacca acgcaaaggt ttctcctgta atagcagccg gtaaaacccg
     5701 gctacctgaa tgggttgcga atcgcgttca gcttatattg tggtcattag caaaatttca
     5761 agatgtttgc gcaactattt ttggtagtaa tcccaaagcg gtgatctatt tcacaaatta
     5821 ataattaagg ggtaaaaacc gacacttaaa gtgatccaga ttacggtaga aatcctcaag
     5881 cagcatatga tctcgggtat tcggtcgatg caggggataa tcgtcggtcg aaaaacattc
     5941 gaaaccacat atattctgtg tgtttaaagc aaatcattgg cagcttgaaa aagaaggttc
     6001 acatgtcaaa caacattcgt atcgaagaag atctgttggg taccagggaa gttccagctg
     6061 atgcctacta tggtgttcac actctgagag cgattgaaaa cttctatatc agcaacaaca
     6121 aaatcagtga tattcctgaa tttgttcgcg gtatggtaat ggttaaaaaa gccgcagcta
     6181 tggcaaacaa agagctgcaa accattccta aaagtgtagc gaatgccatc attgccgcat
     6241 gtgatgaagt cctgaacaac ggaaaatgca tggatcagtt cccggtagac gtctaccagg
     6301 gcggcgcagg tacttccgta aacatgaaca ccaacgaagt gctggccaat atcggtctgg
     6361 aactgatggg tcaccagaaa ggtgaatatc agtacctgaa cccgaacgac catgttaaca
     6421 aatgtcagtc cactaacgac gcctacccga ccggtttccg tatcgcagtt tactcttccc
     6481 tgattaagct ggtagatgcg attaaccaac tgcgtgaagg ctttgaacgt aaagctgtcg
     6541 aattccagga catcctgaaa atgggtcgta cccagctgca ggacgcagta ccgatgaccc
     6601 tcggtcagga attccgcgct ttcagcatcc tgctgaaaga agaagtgaaa aatatccaac
     6661 gtaccgctga actgctgctg gaagttaacc ttggcgcaac agcaatcggt acaggtctga
     6721 acacgccgaa agagtactct ccgctggcag tgaaaaaact ggctgaagtc actggcttcc
     6781 catgcgtacc ggctgaagac ctgatcgaag cgacctctga ctgcggcgct tatgttatgg
     6841 ttcacggcgc gctgaaacgc ctggctgtga agatgtccaa aatctgtaac gacctacgct
     6901 tgctctcttc tggcccacgt gccggcctga acgagatcaa cctgccggaa ctgcaggcgg
     6961 gctcttccat catgccagct aaagtaaacc cggttgttcc ggaagtggtt aaccaggtat
     7021 gcttcaaagt catcggtaac gacaccactg ttaccatggc agcagaagca ggtcagctgc
     7081 agttgaacgt tatggagccg gtcattggcc aggctatgtt cgaatccgtt cacattctga
     7141 ccaacgcttg ctacaacctg ctggaaaaat gcattaacgg catcactgct aacaaagaag
     7201 tgtgcgaagg ttacgtttac aactctatcg gtatcgttac ttacctgaac ccgttcatcg
     7261 gtcaccacaa cggtgacatc gtgggtaaaa tctgtgccga aaccggtaag agtgtacgtg
     7321 aagtcgttct ggaacgcggt ctgttgactg aagcggaact tgacgatatt ttctccgtac
     7381 agaatctgat gcacccggct tacaaagcaa aacgctatac tgatgaaagc gaacagtaat
     7441 cgtacagggt agtacaaata aaaaaggcac gtcagatgac gtgccttttt tcttgtgagc
     7501 agtaacttaa aaataacaat ctaatatcaa cttgttaaaa aacaaggaag gctaatatgc
     7561 tagttgtaga actcatcata gttttgctgg cgatcttctt gggcgccaga ttggggggaa
     7621 taggtattgg ttttgcaggc ggattggggg tgctggttct tgccgctatt ggcgttaaac
     7681 ccggtaacat cccgttcgat gtcatttcca ttatcatggc ggttatcgcc gctatttctg
     7741 ccatgcaggt tgctggcggt ctggactatc tggttcatca gacagaaaag ctgctgcgcc
     7801 gtaacccgaa atacatcacg atcctcgcac cgatcgtgac ctatttcctg actatctttg
     7861 ctggtactgg caacatctct ctggcgacac tgccagttat cgctgaagtt gcgaaggaac
     7921 aaggcgttaa accttgccgt ccgctgtcta ctgcagtggt atccgcgcag attgcgatca
     7981 ccgcatcgcc aatctcagcg gcagtggttt acatgtcttc cgtgatggaa ggtcatggca
     8041 tcagctacct ccatctgctc tccgtggtca tcccgtccac cctgctggcg gttctggtga
     8101 tgtcctttct ggtcactatg ctgttcaact ccaaactctc tgacgatccg atttatcgca
     8161 agcgtctgga agagggcctg gttgaactgc gcggtgaaaa gcagattgaa atcaaatccg
     8221 gtgcaaaaac gtccgtctgg ctgttcctgc tgggcgtagt tggcgtggtt atctatgcaa
     8281 tcatcaacag cccaagcatg ggtctggttg aaaaaccgct gatgaacacc accaacgcaa
     8341 tcctgatcat catgctcagc gttgcaactc tgaccaccgt tatctgtaaa gtcgataccg
     8401 acaacatcct caactccagc accttcaaag caggtatgag cgcctgtatt tgtatcctgg
     8461 gtgttgcgtg gctgggcgat actttcgttt ccaacaacat cgactggatc aaagataccg
     8521 ctggtgaagt gattcagggt catccgtggc tgctggccgt catcttcttc tttgcttctg
     8581 ctctgctgta ctctcaggct gcaaccgcaa aagcactgat gccgatggct ctggcactga
     8641 acgtttctcc gctgaccgct gttgcttctt tcgctgcggt gtctggtctg ttcattctgc
     8701 cgacctaccc gacgctggtt gctgcggtac agatggatga cacgggtaca acccgtatcg
     8761 gtaaattcgt cttcaaccat ccgttcttca tcccgggtac tctgggtgtt gccctggccg
     8821 tttgcttcgg cttcgtgctg ggtagcttca tgctgtaatg accaatcgcg gggcgttcac
     8881 gccccgcttt ctttcccgcc gactaacatc cttcccccgt ccgttgtata gtgacctctc
     8941 tcttgcggtt ccatctgttc ttgcgaggtg tttatgcttg atgaaaaaag ttcgaatacc
     9001 gcgtctgtcg tggtgctatg taccgcaccg gatgaagcga cagcccagga tttagccgcc
     9061 aaagtgctgg cggaaaaact ggcggcctgc gcgaccttga tccccggcgc tacctctctc
     9121 tattactggg aaggtaagct ggagcaagaa tacgaagtgc agatgatttt aaaaactacc
     9181 gtatctcacc agcaggcact gctggaatgc ctgaagtctc atcatccata tcaaaccccg
     9241 gaacttctgg ttttacctgt tacacacgga gacacagatt acctctcatg gctcaacgca
     9301 tctttacgct gatcctgcta ctttgcagca cttccgtttt tgccggatta ttcgacgcgc
     9361 cgggacgttc acaatttgtc cccgcggatc aagcctttac ttttgatttt cagcaaaacc
     9421 aacatgacct taatctgacc tggcagatca aagacggtta ctacctctac cgtaaacaga
     9481 tccgcattac gccggaacac gcgaaaattg ccgacgtgca gctgccgcaa ggcgtctggc
     9541 atgaagatga gttttacggc aaaagcgaga tttaccgcga tcggctgacg cttcccgtaa
     9601 ccatcaacca ggcgagtgcg ggagcgacgt taactgtcac ctaccagggc tgtgctgatg
     9661 ccggtttctg ttatccgcca gaaaccaaaa ccgttccgtt aagcgaagtg gtcgccaaca
     9721 acgcagcgtc acagcctgtg tctgtttcgc agcaagagca gcacaccgcg caattgccct
     9781 tttccgcgct ctgggcgttg ttgatcggta ttggtatcgc ctttacgcca tgcgtgctgc
     9841 caatgtaccc actgatttct ggcatcgtgc tgggtggtaa acagcggctc tccactgcca
     9901 gagcattgtt gctgaccttt atttatgtgc aggggatggc actgacttac acggcgctgg
     9961 gtctggtagt tgccgccgca gggttacagt tccaggcggc gctacagcac ccatacgtgc
    10021 tcattggcct cgccatcgtc tttaccttgc tggcgatgtc aatgtttggc ttgtttactc
    10081 tacaactccc ctcttcgctg caaacacgtc tcacgttgat gagcaatcgc caacagggcg
    10141 gctcacctgg cggtgtgttt gttatggggg cgattgccgg actgatctgt tcaccatgca
    10201 ccaccgcacc gcttagcgcg attctgctgt atatcgccca aagcgggaac atgtggctgg
    10261 gcggcggcac gctttatctc tatgcgttgg gcatgggcct gccgctgatg ctaattaccg
    10321 tctttggtaa ccgcttgctg ccgaaaagca gcccgtggat ggaacaagtc aaaaccgcgt
    10381 ttggttttgt gatcctcgca ctgccggtct tcctgctgga gcgagtgatt ggtgatgtat
    10441 ggggattacg cttgtggtcg gcgctgggtg tcgcattctt tggctgggcc tttatcacca
    10501 gcctacaggc taaacgcggc tggatgcgcg tggtgcaaat aatcctgctg gcagcggcat
    10561 tggttagcgt gcgcccactt caggattggg catttggtgc gacgcatacc gcgcaaactc
    10621 agacgcatct caactttaca caaatcaaaa cggtagatga gttaaatcag gcgctcgttg
    10681 aagccaaagg caaaccggtg atgttagatc tttatgccga ctggtgcgtc gcctgtaaag
    10741 agtttgagaa atacaccttc agcgacccgc aggtgcaaaa agcgttagca gacacggtct
    10801 tacttcaggc caacgtcacg gccaacgacg cacaagatgt ggcgctgtta aagcatctta
    10861 atgtccttgg cctaccgaca attctctttt ttgacggaca aggccaggag catccacaag
    10921 cacgcgtcac gggctttatg gatgctgaaa ccttcagcgc acatttgcgc gatcgccaac
    10981 cgtgaacgac acttgcagtg ggaaagacgg aggaaaatag cgtgcaacgt gaagatgtac
    11041 tgggagaagc cctgaaatta ttagaattac aagggattgc caacaccacg ctggagatgg
    11101 ttgctgaacg tgtggattat ccactggacg agctacaccg cttctggcca gacaaagagg
    11161 caatcctcta cgatgcgctg cgttatctta gccaacagat agatgtctgg cgtcgtcagc
    11221 tgatgctgga cgaaacgcaa accgccgaac aaaagttgct ggcacgttat caggcgttat
    11281 cggagtgcgt taaaaacaac cgctatccgg gctgtctatt tatcgctgcc tgtacgtttt
    11341 atcccgatcc tggccaccct attcatcaac tggccgatca gcaaaaaagc gcggcctacg
    11401 atttcaccca cgaactgtta accacgctgg aagttgacga tccggcgatg gtagcaaagc
    11461 agatggaact ggtgctggaa ggctgtttaa gccgaatgct ggtgaatcgt agccaggcgg
    11521 atgtcgacac tgcacatcgg ctggcggaag atatcctgcg cttcgcccgc tgccgcctgg
    11581 gtggtgcatt gacctgacag aaacacagaa aagaagcgat ttgccgcaat cttaagcagt
    11641 tgaatcgctt ttactgaaat taggttgacg agatgtgcag attacggttt aatgcgcccc
    11701 gttgcccgga tagctcagtc ggtagagcag gggattgaaa atccccgtgt ccttggttcg
    11761 attccgagtc cgggcaccaa attcatatca acggacctcc acggaggtcc gtttttcgtt
    11821 tcagaacacc atgatttaag tgttcttcct ccaaatcaat tctaccgaac tcaaccaaat
    11881 tctccccaca tcaa
//
LOCUS       DAZKHC020000115        11879 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZKHC020000115 DAZKHC020000000
VERSION     DAZKHC020000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.115"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..216
                     /locus_tag="ACHOSM_RS19635"
                     /old_locus_tag="pgaptmp_003927"
     CDS             <1..216
                     /locus_tag="ACHOSM_RS19635"
                     /old_locus_tag="pgaptmp_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560331.1"
                     /translation="MRDIIEYAIKENLGAREALSIMEREINLINEAVSLENKIAREEY
                     VRRRLGVDGSAILTSEHYAKIFNLFSR"
     gene            complement(197..436)
                     /locus_tag="ACHOSM_RS19640"
                     /old_locus_tag="pgaptmp_003928"
     CDS             complement(197..436)
                     /locus_tag="ACHOSM_RS19640"
                     /old_locus_tag="pgaptmp_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560332.1"
                     /translation="MSDIKNALDVAAFTMAGNLMVTLFDKGVISREEATHIINIAQKS
                     AAPQSAEGHQDGSYINTNWHFDNLRACMGITAKKD"
     gene            507..1019
                     /locus_tag="ACHOSM_RS19645"
                     /old_locus_tag="pgaptmp_003929"
     CDS             507..1019
                     /locus_tag="ACHOSM_RS19645"
                     /old_locus_tag="pgaptmp_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HGU4560333.1"
                     /translation="MNRASLITLIHVAKRDLQLDRETYTSALLAATGKTSCRDMSPDE
                     LSRVLDVLKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGAETALNRWV
                     KSQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAV
                     CRQYEKSLPR"
     gene            1089..1514
                     /locus_tag="ACHOSM_RS19650"
                     /old_locus_tag="pgaptmp_003930"
     CDS             1089..1514
                     /locus_tag="ACHOSM_RS19650"
                     /old_locus_tag="pgaptmp_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HGU4560334.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            1586..2086
                     /locus_tag="ACHOSM_RS19655"
                     /old_locus_tag="pgaptmp_003931"
     CDS             1586..2086
                     /locus_tag="ACHOSM_RS19655"
                     /old_locus_tag="pgaptmp_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4560335.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            2121..2549
                     /locus_tag="ACHOSM_RS19660"
                     /old_locus_tag="pgaptmp_003932"
     CDS             2121..2549
                     /locus_tag="ACHOSM_RS19660"
                     /old_locus_tag="pgaptmp_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560336.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASAPVPDDVADRVRRAVCEIRACEAGADPR"
     gene            2533..2751
                     /locus_tag="ACHOSM_RS19665"
                     /old_locus_tag="pgaptmp_003933"
     CDS             2533..2751
                     /locus_tag="ACHOSM_RS19665"
                     /old_locus_tag="pgaptmp_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560337.1"
                     /translation="MPIPAEWLADCLVPPVPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            2762..2989
                     /locus_tag="ACHOSM_RS19670"
                     /old_locus_tag="pgaptmp_003934"
     CDS             2762..2989
                     /locus_tag="ACHOSM_RS19670"
                     /old_locus_tag="pgaptmp_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU4560338.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            2970..3281
                     /locus_tag="ACHOSM_RS19675"
                     /old_locus_tag="pgaptmp_003935"
     CDS             2970..3281
                     /locus_tag="ACHOSM_RS19675"
                     /old_locus_tag="pgaptmp_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HGU4560339.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTN
                     E"
     gene            3274..3564
                     /locus_tag="ACHOSM_RS19680"
                     /old_locus_tag="pgaptmp_003936"
     CDS             3274..3564
                     /locus_tag="ACHOSM_RS19680"
                     /old_locus_tag="pgaptmp_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HGU4560340.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRVLINGYFIAELTGRGQDVAEGRASVPGVKKPRARG"
     gene            3567..4148
                     /locus_tag="ACHOSM_RS19685"
                     /old_locus_tag="pgaptmp_003937"
     CDS             3567..4148
                     /locus_tag="ACHOSM_RS19685"
                     /old_locus_tag="pgaptmp_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HGU4560341.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKV"
     gene            4148..5812
                     /locus_tag="ACHOSM_RS19690"
                     /old_locus_tag="pgaptmp_003938"
     CDS             4148..5812
                     /locus_tag="ACHOSM_RS19690"
                     /old_locus_tag="pgaptmp_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560342.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            5812..7401
                     /locus_tag="ACHOSM_RS19695"
                     /old_locus_tag="pgaptmp_003939"
     CDS             5812..7401
                     /locus_tag="ACHOSM_RS19695"
                     /old_locus_tag="pgaptmp_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HGU4560343.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAVLDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIS
                     DPWIREQTGIPQPTPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIDAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            7385..8716
                     /locus_tag="ACHOSM_RS19700"
                     /old_locus_tag="pgaptmp_003940"
     CDS             7385..8716
                     /locus_tag="ACHOSM_RS19700"
                     /old_locus_tag="pgaptmp_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HGU4560344.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGVHISWNWFETDADVHARS
                     FTAARVARLDVLTTLQAEVQRAVDEGISQKAFISTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLARPKAVLWDKRHNNLMYIVENQDG
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGDID"
     gene            complement(8718..8797)
                     /locus_tag="ACHOSM_RS19705"
                     /old_locus_tag="pgaptmp_003941"
     tRNA            complement(8718..8797)
                     /locus_tag="ACHOSM_RS19705"
                     /old_locus_tag="pgaptmp_003941"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            8838..9311
                     /locus_tag="ACHOSM_RS19710"
                     /old_locus_tag="pgaptmp_003942"
     CDS             8838..9311
                     /locus_tag="ACHOSM_RS19710"
                     /old_locus_tag="pgaptmp_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HGU4560345.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLTRSVASSLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            9488..10612
                     /locus_tag="ACHOSM_RS19715"
                     /old_locus_tag="pgaptmp_003943"
     CDS             9488..10612
                     /locus_tag="ACHOSM_RS19715"
                     /old_locus_tag="pgaptmp_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HGU4560346.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIEGHPAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDESTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQLLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTGLQTD
                     TVTVPDKKTATAVLSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            10612..11559
                     /locus_tag="ACHOSM_RS19720"
                     /old_locus_tag="pgaptmp_003944"
     CDS             10612..11559
                     /locus_tag="ACHOSM_RS19720"
                     /old_locus_tag="pgaptmp_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HGU4560347.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAVQPDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            11603..>11879
                     /locus_tag="ACHOSM_RS19725"
                     /old_locus_tag="pgaptmp_003945"
     CDS             11603..>11879
                     /locus_tag="ACHOSM_RS19725"
                     /old_locus_tag="pgaptmp_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HGU4560348.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEAEPCLTVRILQTSADDAGSVAGVVHAVAGTD"
BASE COUNT         2699 a         3160 c         3518 g         2502 t
ORIGIN      
        1 atgcgtgaca ttatcgaata tgcgataaaa gaaaatcttg gagccagaga ggcactgtcg
       61 ataatggagc gggaaattaa tctgattaat gaagcggttt ctctggaaaa caaaatagcc
      121 cgcgaggaat acgttcgccg caggcttggt gttgatggtt cagcaattct tacatctgaa
      181 cattatgcaa aaatttttaa tcttttttcg cggtaatgcc catgcaggcc cggagattgt
      241 cgaaatgcca gtttgtatta atatatgagc catcctggtg tccctctgcg ctttggggtg
      301 cggcactttt ttgagcaata ttgataatat gtgttgcctc ctcacgggaa ataaccccct
      361 tatcgaacag ggttaccata agatttccag ccattgtgaa tgcggcaaca tcaagggcat
      421 tttttatatc tgacatagat ttttcctttt atgttaattg acatggcggt gcggtaacac
      481 cgcccatttt ttatggagcc acgataatga atcgcgcttc cctgattacc ttaatacatg
      541 tcgcaaaacg cgatctacag cttgaccggg agacctatac atccgcgctg ctggcagcca
      601 ccggcaaaac cagctgccgg gatatgtcac cggatgagtt atcccgcgtg ctggatgttt
      661 taaaaaaacg cggttttaaa gtgcgccaga agccggttaa ccgggcctta aaaccgggta
      721 cggtgaccgc caaaattcgt gccatctgga aggtgatgca ccggcagggc tttatctctg
      781 atggtgcgga aaccgctctt aaccgctggg tgaaatcgca gacggccgcg cagaacggcg
      841 gcgaaggtgt ggcaaactgg cagtggctgg agcaacaccc cgccctggcc tcagatgtgc
      901 tggagcgtct caagcgatgg caccgccgca aaatgctggc cgcgatggga atgcccgaac
      961 gcacgctgat ggggtatgac gccgtttgca ggcagtatga aaaatcactt ccccgttaac
     1021 cccaaatccc gccacaacgc gggattttta ttttaaactt accgggaacg cgagaaccgg
     1081 aggctgatat ggcagaaact cagatgagca tgtttggtgg tgacagtgag caactgcacg
     1141 cccttatcga ccgccttgat gacatccctg atgatgttct caaaaagaac tggccgcgga
     1201 ccctgtcaga actggttgaa gtcaccggcg cagaacttca gcgtcagggg attgagccgg
     1261 tactggccgg taaactggca cgcaaggtgg ctgcggctca ggcagcctat atgggcggac
     1321 ggggttatta cctgccggtc ggggaatctc tctttgccga gctgcgaaac aatgagatat
     1381 tttcgcgctg ggaccggggc gagaaaattg aatcccttcg ccgccattac cggatgtcag
     1441 aaacccagat ttataccgtc atacgtgaac agcgccgtct gcatctggca agaacgcaac
     1501 cgccactttt ctgatattcc gcaaagcctg ccgcctttcc ttcaccgtta cgctgactca
     1561 gagaacatca tgagacagcg taacaatgcc aaaactcccc gcaccactgc gtaaaaagct
     1621 gattgccctt gttctggccg gtgccgggac gttcacgatt gccacgcatt acaccggtta
     1681 ctgggaaggg aaagaaaact ccacgtatat cgatcccacc ggtacaccca ccatctgtta
     1741 cggccatacc ggcccggatg tgaaaccggg tatgaccctg acggatgaag aatgcctgga
     1801 actactggaa aaggacatga aatgggcctt tgcggccatt gatcggcgtg ttcaggtgcc
     1861 gcttacccgt ggtcagactg tggcgctggc ttcgtggatc ttctgggccg gtgaaacgaa
     1921 cttccgcaac tccacgcttc tgcgcctgat caatgccggg cagatgcccg cgtcctgtaa
     1981 gcagtatatc cgctggattt attcaaaggg ggtgaaactc cccggccttg aggcccgccg
     2041 ttcggcggat gaatggttat gtcgctacga cttgccgaaa gtctgaaccg cttctggcga
     2101 ccgctcatga tagcgctgct gtgtgcggta ctgctgttac ggggtgtcct gtggctgcgg
     2161 tgaataatgc cttaccggcc ctgattgccg gggcgtgcat ggcggcgctg gggattatcg
     2221 ctgtactggt tgccgcgata tggggaatgc accagaagac gcagcgcctt gaagacaata
     2281 atcaggtgct tgtgcgtgag cgggatgagg cgcgtcaggt gctggcaaat cagcagcaca
     2341 ccctgcaact catttcacaa atcagtgagg cggccacgaa tgaaaaacaa cagaacattc
     2401 agcacagcga gggacagcag agcgttgtcc gccggtcgct ggcagcggtg cctgcggcct
     2461 ctgcccctgt tcctgatgat gtggctgatc gggtgcgccg ggccgtctgt gaaatacgtg
     2521 cctgtgaagc cggtgccgat ccccgctgaa tggctggctg actgcctggt ccctcctgtg
     2581 ccggagccgt tcacgtttgg ggcatcggtc acttacaacc tgcaactgct ggcggtgatc
     2641 aagaactgca acgtggacaa ggccagtatt cgtcgtctgg aggcgcggcg acaacatgaa
     2701 tttactgata tggccggaac gcctgctgtt ccggcaggaa agacgaagta aggaaaaagg
     2761 tatggatgat tcagatcgtg ctcaggctgt gatggagcgg ggatcagaac gcgccctttg
     2821 taaccggctg acacgtaagc gccgggcaat ggatgctccg ggcagacgtg tctgcgcgga
     2881 ctgtggcgga gagatccccg ccgcacgtct tgttgctgtg ccggatgcca tccgttgtgt
     2941 gaactgccag aacatcatgg aggcccgtca tgtgggtcag catcgttaaa gattatgttg
     3001 tgccgatcct ttcggcgacg gcaaccgctg gcgggatatt catggcgctg atgcgcaaaa
     3061 cgtttgtccc ccgcgaggcc tttgaaaaac tctccgaccg cgttgagaag gtggaaaccc
     3121 gcctgtcgtc actgccgaca gaagctgagg tcaaccgtct gaatgtggaa atcgtgaccc
     3181 tgcggggtga actgaaaacc acgaacgcca cgctccgctc tgtctcctat cagaacgaac
     3241 tgctgctgga gcaggcggta aggaaaaaaa cgaatgagtg atttcattac tgaagatcag
     3301 cgtctggtca ttctgcggtc gctggcagat tacaacggtg aactgggtga atccgtgctc
     3361 caggactgtc tggatgatta cggccaccgc gtgtcccgcg acaccgttca cacccacatt
     3421 gcctggcttg ccgagcaggg actggtgcgc aaacgcgttc tgattaacgg ttatttcatc
     3481 gcggaactga ccggccgggg gcaggatgtg gcggaaggcc gcgccagcgt tccgggtgtg
     3541 aagaaaccac gcgcaagggg gtaacgatgg acaagccgac gcgtggacgc gtgcgcaagg
     3601 tggatttgct ccccgacagc atccgtaagc cactgctgga aatgctgcgt gaaaaacgtc
     3661 tgacgcaggt ccagatacgc gaggaaatca accgtctgat ccgtgaggcg ggcctgccgg
     3721 aagagcagca gctttcaccg gcggccatca gccgggaagc ctcccgtaat gagctgattg
     3781 cccgtaacct gcgtgattta cgcgagcaga caaaagccat gatggccgaa ctgggggaca
     3841 agccaaccgg tgaaaccact gcattgatcc tggagatgtc ccgcgccctg atgtaccgcc
     3901 gtctgcgtgc cgcgaccgaa tccctgaaca gtgacagcga tgtggatatg cgtcttatta
     3961 aggacatcct cctgtcagca cagcgtgcgg aaagtgccgc cgagcgcagc attaagcgtg
     4021 aaaaagaaat cagggctgca tttgctgaag agatggcaaa cgccgtcacc gacgagctgc
     4081 gcggcgtgga cgggatgagt gaacagcttg agtcccgtat taaggggatt ctgttgggta
     4141 aagtctgatg aataacgccg aagaactctc ccccttactg acgaataccg tctccacccg
     4201 caaaattgat ctggccggtg aaaaagccct actgggcgtg gatgtgccgg acagccttga
     4261 tttgcccggt gatatgccgg tatttctgga ttatcaggcc cgctggtttg aggatgaaag
     4321 cgaggtctgc atcgcggaaa aatcccgccg caccgggctt acctgggcgg aagccgggcg
     4381 taacgtcatt accgctgcaa aaccgaagcg gcgcggtggc cgcaatgtgt tttatgtggg
     4441 gtcaaagcag gagatggcgc tggaatatat ttctgcctgt gcactgttct cccgtgcctt
     4501 taaccagctg gcagatgctg atgtgtatga gcagaccttc tgggatcggg ataaaaaaga
     4561 agaaattctg acctacatga tccgctttcc gaacagtgga ttcaaaattc aggcactgtc
     4621 ttcccgtccg tcaaacctgc gcggccttca gggggatgtg gtgattgatg aagccgcgtt
     4681 ccacgagtca ctggatgagc ttctcaaggc ggcaatggcg cttaccatgt ggggcgcgcg
     4741 cgtgcgtatt atctccacgc ataacggcgt cgataatctg tttaatcagt acattcagga
     4801 agcccgcgaa gggcgcaagg attacagcgt ccaccgcatt accctggatg atgccattgc
     4861 ggacgggctg taccgtcgta tctgttatgt caccggccgc gaatggtcac cggaaagcga
     4921 gcagaagtgg cgtgatgatc tctacaaaaa cgccccgacc cgtgaggatg ccgacgagga
     4981 atacggctgt atcccgaaaa aatccggcgg tgcctatatt cctcacgcgc ttattgaaat
     5041 ggcgatgatc cgcgacatcc cgattctgac gtttgaagcc ccggacgact tcatcagccg
     5101 tgccgcatgg ctgcgtgaat cggaggtttt aacctggtgt gaagaacatt taaaaccgct
     5161 tttagaggcg ttaaatcccc gttcccgatt cagcttcggg gaagactttg cccgcaccgg
     5221 agacctgtcc tgttttgtgc tgctggaaat cactgaatcc ctggcaaaac gcgaggtgtt
     5281 tcgcgtggag ctgcgcaacc tgccgtatgc ccagcaggag caggtgatga tgtacatcct
     5341 gacccgcgtt ccggcgctgg tcggtgcggc gttcgacgcc accggtaacg gcggctatct
     5401 ggccgaagcc gcgctgctgg cctttggccc ggacatcatc gactgcgtga tgttatcgcc
     5461 gaagtggtac ggcgagtgga tgccaaaact gaaggccgag tttgaggatc agaatatcct
     5521 cgtcgcccgc catcagacca cgcttgatga tttgcgccat gtgaaggtgg tgaacggtat
     5581 cccgcaaatc gacaaggggc gtacgaagga ccagaacgca acggctgtga acgcccgccg
     5641 ccatggtgat tttgccgtgg cgctctgtat ggcaaaccgg gcgtcataca tggagggctt
     5701 tatcctggat gaatcagcct gccaggcgct gccggagcgg tcgcgggcca tggagggcgg
     5761 ttatcgtgat gacgatgagg catatcatga atttgatcgg gggtgctggt agtgggacgc
     5821 ataattgatc ttgacggaaa acctttctcc tttgacccgg agatgcagag tgccgtgctg
     5881 gatattccgc agattgccag ccgttatatt gaacatccgg cctcgggtat caccccgaac
     5941 cgggcggcac agtgccttcg cggggctgaa cgtggcgatc tgattgccca gtccgatctg
     6001 gccgctgaca ttgaagaaaa ggatacccat ctttttgcag agctgggcaa gcgacgtctt
     6061 gccattcagg gggtgccctg gagtattgag ccgcccccga acgccagcgc gaatgagaaa
     6121 aaggacgcgg aaatgctcga cgaatacctg cattccgccg actggtttga tgccatgctg
     6181 tttgatgcca ccgatgccat cctgaaaggc tattcctgca tggagattga gcacgggatg
     6241 ctcggtaaaa tgcacatcat ccgcgccatc cgctggcgtg acagcgggca tttctgcctt
     6301 aacccggatg atttgagcga actgcggctg cgtgacggca gccatgccgg ggtggcgttt
     6361 cagccctttg gctggatagt gcatcagtca cgttcacgca ccggttacgg cggcgcaaca
     6421 gggcttgtca gaacgcttat ctggccgttc attttcaaaa actattccgt gcgcgatctg
     6481 gctgaatttc tggaggtgta cggcctgccg atgaaggtcg gtaaataccc gtccggggca
     6541 acaccggagc agaaaagcgc cctgatgcgg gcggtgatgg atatcgggcg acgtacaggc
     6601 gggatcatcc cggccgggat gtcgctggag tttcaggcag ccgcgaacgg tcaggccgat
     6661 ccgtttgaaa ccatgatttc gtggggggag cgttccatct ccaaagcaat actcggcggc
     6721 acgctgacca cggaagccgg agacaaaggc gcgcgctcgc tgggtgaagt gcataacgag
     6781 gtgcgccggg aaatcaggga ttctgattta cgccagctgg ccgccacgct gaaccgcgat
     6841 ctggtgtatc cgctgtatgc cctgaacacc gcccacgcca ttgatatccg tcgcctgccg
     6901 cgtatctgtt tccagacaaa agagccgggg gatatcacca aaatcaccag tgcggtgatg
     6961 cagctcagta cggggatgga tatttctgat ccctggataa gggagcagac cggtattccg
     7021 cagcccacgc ccggtgaagc catcttccgt gtccgtcaga gtggcaatga acctgctcag
     7081 acagacaaag agatgccacc ggaaaaacag gagaagacag agcagacagc gctggctgcc
     7141 agactgccgg aagcgaaaag cagcccccgt gatgaactgg acgacatggg ggatgcggtg
     7201 cctgcccgcc ggttacagga ggccatcgac ccggtactgg agccggtcat tgatgccatc
     7261 agaacgcggg ggctggcgga ggcactggca gacctgcccg ccctttaccg tgaaatggat
     7321 gattcccgcc tgatgacgct gctcagtgat gccatgtttg ctgcggaaat gaaggggatg
     7381 ctggatggca caggggattg atttaggtta tgccgccacc cttccctcaa aagaggcggt
     7441 ggcatacttc cgcgccaaag gggtgcatat cagctggaac tggttcgaaa cagacgcgga
     7501 tgttcatgcc cgttcattca cggcggcaag agtggcacgc ctggacgtac tgaccacact
     7561 acaggcggaa gtgcagcgtg ctgttgatga ggggatttca cagaaagcat ttatcagcac
     7621 gctgacaccc cgcctgcaaa agctgggatg gtggggaaaa cagattgtgg tggacagcgc
     7681 cggtaacgca gaagaagtgc agctgggcag tccccgccgt ctggcgctga tttacaacgt
     7741 aaacacccgt gtggcttaca atgccgggcg ttacacgcag atgatgaaca acacggacac
     7801 gcatccgttc tggcagtatg tggcggtgat ggacagccgc acccgcccgg cgcattccgc
     7861 tcttaacggt ctggtattcc gctatgacga tccgttctgg aatacacact acccacccaa
     7921 tggctggaac tgtcgctgcc gtgtccggcc attgtctcag gcccgtctgg atgcaatggg
     7981 gttatccgtt tcatccggtc aggatcatct ctccacccgc aatgttgagg ctggcgtgga
     8041 taagcagacc ggggaagtca gagaaatgcc ggtgaccaca tattcagatg gcaccagaac
     8101 catgacaccg gatgtgggct ggtcatataa ccccggttcg gcggcgttcg gcacagacca
     8161 ggcgctgatc cgtaaactga tcgaggtgaa aagcccggcg ctgcgggaaa tggtggttca
     8221 ggagatgaac aacagcccgg agcggcaact ggcgttccgc atctgggcaa aaaacatcat
     8281 gaaaacccgg cgaggcggta acgatatccg cacgctgggc tttatgaccg aaagcattgc
     8341 gcaggcggtg gaaagccgga cgggaacgcc accggcccgc ctgctggcga tgagcggtaa
     8401 aaatgtgctc catgcggaca gcatgaaaca tcagaatgac ggtatcgccc tgacgccgga
     8461 ggatttcggg cgcttaccgg caatgctggc aaggccaaaa gcggtactgt gggacaaacg
     8521 gcacaacaat ctgatgtata tcgtggaaaa tcaggatggc agtgtgcaga ttgccgttaa
     8581 tgcaccgtac agcctgaagc gtcagccgga taaactggat gtgattgtta atgcttaccg
     8641 ggttatcaat atggataagc tgaaatcgga tatccggggc ggaatgcttg aggtactgga
     8701 aggagatatt gattaacggt cagtggcggg ggtcgaaccc gcatacatga tccacccgga
     8761 ggcaggcacc gactttacca gttaagcgta cactgaccgc ctgtttattt tatattcatc
     8821 ctttatgcgg aggcaatatg tcatccattg atgccgctgt cgttgttgac gttacgcgtc
     8881 tccagcgggt ctttgcccgg cttcagtttg tgggtggtgg aaaagacctg acccgcagtg
     8941 tggcgtccag ccttctgtca tcgtcagaaa tggcgtttga acaggaaaaa gagccggacg
     9001 gcgaacgctg gcatgactgg tcagatcctt accgcaagtg gcgtacccgt aagggataca
     9061 tgcccggcaa aatcctgacg ctgaacggcg atctggcccg acgcctgacc acggattatg
     9121 gcgatacctg ggcgctgatt ggatcaaatg agccttatgc ggccattcat cagtgggggg
     9181 gcctgccagg tatgccgcct ggaccggcgg ccattggtgc acgtccgtat atgggctttg
     9241 atcaggtggc agagcaggag atcatggacg aaatcagaaa acgctttaaa aaggccacag
     9301 aaacgcctta attgtttaag gtatgcaaat gcattacctt acccccttca ggcgcgtgtc
     9361 gtgattttct aacctgaatt taacgggctt taaaatctgc gtgaagcgcc tttgtatttt
     9421 tcccctgaaa gcacttcctg aaatcccgca aaacccgccc tgaaaattcc cggcctatgc
     9481 tgccggaatg aagacgaaaa acacgcccaa acttgcttat gccattctga atgccatcag
     9541 cctgtccgca gacggggacg gtgactggtg tcagatcatg ccagccggtc gggtaaaggc
     9601 ccgtgacggt cgcccggaaa aaccggccga gggctggctg atcaaccatg cagccgttga
     9661 gcgcatggtc tcccgtgttg tggcgctcaa tcagccggtg aaaattgact acaaccacca
     9721 gaccctgatt gagggacatc cggcaccggc tgccggtttt gtgatggcct caccggagaa
     9781 ttttcgcttc agtgaagagc gaggtttcga ggtgcgcccg aagtggaacc ctccggccct
     9841 tgaacatctg cgtaataacg aattcccctg gttttcaccg gtgattggct atgacgagag
     9901 caccggcgaa cctgtcgaac ttcggatgct ggctattacc ggtgaccccg gtctgaccgg
     9961 catgaatcct gtcgccgcac tgtcggcgga tgacctttat aacgccttaa accctccttt
    10021 aaaggatacc tccatgaatg agcaattacg ccagttgctg acggcgctcg gtctgaccgt
    10081 ggctgacggt gacgaattta cgccggagct gggcacggca gcgctgtctg ccctcaccgg
    10141 gatcaaaacc cgtgcggatg cacacgacaa cctgaaaaca caggtcgcca gtctgtcggc
    10201 agagctggaa actgcaaaag gcacaccggc tggcggcagt attgacctga cgaaatacgt
    10261 gcccgttgaa acgtataacg ccctgcgtac cgaatacgtt gcgctgtcgg cgcagcacgg
    10321 cagcaccacg ctggagcagt tgctggacaa ggccgagtcc gaaggacgca tcttcaaaag
    10381 tgaacgcggc tatctggaac aactgggcgg gcagattggc gtggcggcac tttctgcaca
    10441 gcttgacgcc cgccagccgg ttgcggccct gaccggcctt cagaccgaca ccgtgaccgt
    10501 gccggataaa aagactgcca ccgctgtgct gtcggctgaa gatatcgcgg ccgccaaact
    10561 gctgggtaaa accgaagccg agttcctgaa aatgaaagag gaaatgcaat aatgcctacc
    10621 ccgattacac cggcgatgat caccgccctg atgacgggct accgttctga ttttcaggcc
    10681 gggatgtcca tggccccgtc gcagtacaaa aaaattgcga tgaccgtgcc ctccacatcg
    10741 aaatccaaca cctacggctg gctggggcag ttcccgcagt tccgtgagtg gatcggctcc
    10801 cgcgtcatcg agaagatgaa agcctatggc tatgccatcg tgaacaaaac ttttgaaggc
    10861 actgtcgcta ttagccgcga tgacttcgaa gacgacaacc tcggtattta ttccccgttg
    10921 ttccaggaga tggggcgcgc tgcggcggta caaccggatg agctggtctt tgctgccctg
    10981 cgtgacggga tcagtgcggc ctgctatgac ggtcagaact tctttgatac cgaacatccg
    11041 gtttacccca aagtggatgg ctccggggat gcgcagatgg tcagcaatat gtttgtggca
    11101 aaaaccggct ctgtcggcgc acaggctgat tacagcggtc cggcctggta tctgcttgac
    11161 tgctcccgcg cggtaaaacc gctgatttat caggatcgcc gtaaggctga actggttgcc
    11221 cagaccaaag tcgatgaagg tcgcgcattc accgataacg aatttgtgtt tggtgcatcc
    11281 gcccgtcgca atgtgggcta cggcttctgg cagatggcct acatgatgca gtcaccgctg
    11341 acgctggatg cactgtggca cggctggtca gccatgcgcg aatttaccgc tgacggtggc
    11401 cgcaaactcg gcattaaacc cacccatatt gttgtcccca cctcgctgga aaaacaggcg
    11461 gtgcagctgc tggagcgtga actgttcgca gacggaaacg caaccgtctc taacgagatg
    11521 aagggcaagc tggagctggt tgtcgcggat tatctgtaaa cggtgagccg ggcttgcggc
    11581 ccggcccctg cggagggcaa atatgaatga acatcacact gtagcagcaa cagatgattc
    11641 aggtcttcag gtcagtggtg acaattctgt cacggtgctg gcagaagtgc gttgcagtcg
    11701 ttcggcgttt cgtcgtgcgg ggtttctgtt cacgcgtgga cgtcagcagg ttgaggtcac
    11761 cccggagcag cttgcccgac tggaggcgga gccatgcctc actgtgcgaa tactacagac
    11821 gtctgctgat gatgcgggga gcgtggcggg tgtggttcat gcagtggccg gtacagatt
//
LOCUS       DAZKHC020000116        11505 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZKHC020000116 DAZKHC020000000
VERSION     DAZKHC020000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.116"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            323..1189
                     /gene="folD"
                     /locus_tag="ACHOSM_RS19730"
                     /old_locus_tag="pgaptmp_003946"
     CDS             323..1189
                     /gene="folD"
                     /locus_tag="ACHOSM_RS19730"
                     /old_locus_tag="pgaptmp_003946"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HGU4560349.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1191..1403
                     /gene="ybcJ"
                     /locus_tag="ACHOSM_RS19735"
                     /old_locus_tag="pgaptmp_003947"
     CDS             1191..1403
                     /gene="ybcJ"
                     /locus_tag="ACHOSM_RS19735"
                     /old_locus_tag="pgaptmp_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HGU4560350.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1511..2032
                     /locus_tag="ACHOSM_RS19740"
                     /old_locus_tag="pgaptmp_003948"
     CDS             1511..2032
                     /locus_tag="ACHOSM_RS19740"
                     /old_locus_tag="pgaptmp_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4560351.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(2068..3453)
                     /gene="cysS"
                     /locus_tag="ACHOSM_RS19745"
                     /old_locus_tag="pgaptmp_003949"
     CDS             complement(2068..3453)
                     /gene="cysS"
                     /locus_tag="ACHOSM_RS19745"
                     /old_locus_tag="pgaptmp_003949"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGU4560352.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3627..4121
                     /gene="ppiB"
                     /locus_tag="ACHOSM_RS19750"
                     /old_locus_tag="pgaptmp_003950"
     CDS             3627..4121
                     /gene="ppiB"
                     /locus_tag="ACHOSM_RS19750"
                     /old_locus_tag="pgaptmp_003950"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HGU4560353.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4124..4846
                     /gene="lpxH"
                     /locus_tag="ACHOSM_RS19755"
                     /old_locus_tag="pgaptmp_003951"
     CDS             4124..4846
                     /gene="lpxH"
                     /locus_tag="ACHOSM_RS19755"
                     /old_locus_tag="pgaptmp_003951"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HGU4560354.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4964..5473
                     /gene="purE"
                     /locus_tag="ACHOSM_RS19760"
                     /old_locus_tag="pgaptmp_003952"
     CDS             4964..5473
                     /gene="purE"
                     /locus_tag="ACHOSM_RS19760"
                     /old_locus_tag="pgaptmp_003952"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGU4560355.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5470..6537
                     /gene="purK"
                     /locus_tag="ACHOSM_RS19765"
                     /old_locus_tag="pgaptmp_003953"
     CDS             5470..6537
                     /gene="purK"
                     /locus_tag="ACHOSM_RS19765"
                     /old_locus_tag="pgaptmp_003953"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGU4560356.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLDELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6674..7567)
                     /locus_tag="ACHOSM_RS19770"
                     /old_locus_tag="pgaptmp_003954"
     CDS             complement(6674..7567)
                     /locus_tag="ACHOSM_RS19770"
                     /old_locus_tag="pgaptmp_003954"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU4560357.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEKINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7564..8379)
                     /locus_tag="ACHOSM_RS19775"
                     /old_locus_tag="pgaptmp_003955"
                     /pseudo
     CDS             complement(7564..8379)
                     /locus_tag="ACHOSM_RS19775"
                     /old_locus_tag="pgaptmp_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            complement(8390..9649)
                     /locus_tag="ACHOSM_RS19780"
                     /old_locus_tag="pgaptmp_003956"
     CDS             complement(8390..9649)
                     /locus_tag="ACHOSM_RS19780"
                     /old_locus_tag="pgaptmp_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU4560358.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIA"
     gene            complement(9659..11326)
                     /locus_tag="ACHOSM_RS19785"
                     /old_locus_tag="pgaptmp_003957"
     CDS             complement(9659..11326)
                     /locus_tag="ACHOSM_RS19785"
                     /old_locus_tag="pgaptmp_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU4560359.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIAQAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQTACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
BASE COUNT         2573 a         3259 c         3035 g         2638 t
ORIGIN      
        1 atacttccgc gcaaaatata ttcaacacaa atatagacct gaagcggtaa attaccaggc
       61 tgaaaattct ttttatattg tcaggtattt cttaaattat cttaatcctt aaacaaggaa
      121 ataaatcagt tccagattta caacgccatc atggacgaaa aatgaagctt tcaatctcag
      181 cgacctcagc gacggtgcgc ctaaccttcg caagaggtcg cttcacgcga taaatctgaa
      241 acgaaacctg acagcgcgcc ccgcttctga caaaataggc gcatcccctt cgacctacgt
      301 aacagatgga atcctctctc tgatggcagc aaagattatt gacggtaaaa cgattgcgca
      361 gcaggtgcgc tctgaagttg ctcaaaaagt tcaggcgcgt attgcagccg gactgcgggc
      421 accaggactg gccgttgtgc tggtgggtag taaccctgca tcgcaaattt atgtcgcaag
      481 caaacgcaag gcttgtgaag aagtcgggtt cgtctcccgc tcttatgacc tcccggaaac
      541 caccagcgaa gcggagctgt tggagcttat cgatgcgctg aatgccgaca acaccatcga
      601 tggcattctg gttcaactgc cgttaccggc gggtattgat aacgtcaaag tgctggaacg
      661 tattcatccg gacaaagacg tggacggttt ccatccttat aacgtcggtc gtctgtgcca
      721 gcgcgcgccg cgtctgcgtc cctgcacccc gcgcggtatc gtcacgctgc ttgagcgtta
      781 caacattgat accttcggcc tcaacgccgt ggtgattggc gcatcgaata tcgtcggtcg
      841 tccaatgagc atggaactgt tgctcgcagg ttgcaccact accgtgactc accgtttcac
      901 taaaaatctg cgtcaccacg tagaaaatgc cgatctgttg atcgttgccg tcggcaagcc
      961 gggctttatt cctggtgact ggatcaaaga aggcgcaatt gtgattgatg tcggcatcaa
     1021 ccgtctggaa aatggcaaag ttgtgggcga cgtcgtgttt gaagacgcgg ctaaacgcgc
     1081 ctcatacatt acgcctgttc cgggcggcgt tggcccgatg acggttgcca cgctgattga
     1141 aaacacgctt caggcgtgcg ttgaatatca tgatccacag ggtgagtaac atggcgacat
     1201 tttctttagg taaacatccg cacgttgagc tgtgcgattt gctgaaactg gaaggctgga
     1261 gcgaaagcgg cgcgcaggcg aaaatcgcga ttgccgaagg ccaggtgaaa gtcgacggtg
     1321 cggttgaaac gcgcaaacgc tgcaaaatcg tcgccggtca gacagtgagt tttgcaggtc
     1381 acagcgtaca ggttgttgcc tgatcccttt cccggcctgt atcaacaggc cggttctctt
     1441 ttcagacttt tgccctctac gccccgttca tccatgctga tagcatcgac attttgccga
     1501 agcgaccatc atgccaaccg ttattacgca cgccgccgtt cccctatgca ttggtttagg
     1561 gttgggttcg aaagtcatcc ctccgcgtct gttatttgcc ggaatcatcc tggcgatgct
     1621 gcccgacgcc gacgtattgt cgtttaaatt tggcgttgct tacggcaatg tttttggtca
     1681 tcgcgggttt acccattcgc tggtgtttgc gtttgttgtc ccgctgttat gtgtgctgat
     1741 tggacgccga tggttcaggg ctgggctgat tcgctgctgg ctgtttttaa ccgtctcgtt
     1801 gctatcgcac agcttgctgg attcggtaac cactggcggt aagggcgttg gctggctgtg
     1861 gccgtggtca gatgaacgct ttttcgctcc ctggcaggtg attaaagtcg cgccgtttgc
     1921 attgtctcgt tacaccacgc cgtacgggca tcaggtgata atttctgaat tgatgtgggt
     1981 gtggttgccg gggatggtgc ttatgggaat gttgtggtgg cgcaggcgat aaccggatgc
     2041 gaaaactcgc atccggcaac agcgcaatta cttacgacgc caggtggtcc cttgcgggcc
     2101 atcttccagc acgatcccca tctcgttaag acggtcacgc gccgcatctg ccgccgccca
     2161 gtctttcgct ttacgggcat ccagacgctg ttgaattaac gcttcaatct cagccacttc
     2221 gctgtcgtct gcctgcgcgc cgctttgcag gaacgcttcc ggttcttgct ccagcaggcc
     2281 caatacagcg gaaagtttac gcaggtgaga tgccattgca ttcgccgctg ccatatcttc
     2341 tgctttcaga cggtttactt cacgcgccat atcaaacagt acggaatagg cttccggggt
     2401 gttgaaatcg tcgtccatcg cttcaataaa gcgcgcttca aacgcttcgc caccggcagg
     2461 cgcaacggtt ttatctgtgc cgcgcagcgc agtgtagaga cgctccagcg ccgcacgcgc
     2521 ctgcttcagg ttctcttcgc tgtagttcaa ctggctgcga tagtggcccg acatcaggaa
     2581 gtaacgcacg gtttccgcgt cgtagtattt cagcacatcg cgcacggtaa agaagttacc
     2641 cagcgatttg gacatcttct cgcggtcaac catcaccatc cccgagtgca tccagtagtt
     2701 cacatactgg ccatcatggg cacaggtgga ctgcgcgatt tcgttctcgt ggtgcgggaa
     2761 catcaggtct gaaccgccgc cgtggatatc aaagtggtta cccagctgct tgcagttcat
     2821 tgccgaacat tcaatgtgcc agcctggacg gcccgcgccc cacggagacg gccagctcgg
     2881 ttcgccctct ttcgacatct tccacagaac gaagtccatc gggttgcgtt tgtcgtcgac
     2941 cacgtcaacg cgcgcacctg cctgcagctg gtcgagatcc tgacgcgaca gcacgccata
     3001 tgtcggatcg gtcgggacgt cgaacatcac gtcgccgttg tccgccacat aagcgtgacc
     3061 tttggcgatc agttgttcag tgagttcaat aatttctgcg atatggtgcg tcgcgcgcgg
     3121 ctccatatcc gggcgcagaa tgttcaaagc atcaaaatct ttgtgcattt cggcgatcat
     3181 gcgatccacc agcgccacaa agctttcgcc attttcattg gcgcgtttga tgattttgtc
     3241 gtcgatatcg gtaatgttgc gcacatactt cagtttatag ccgaggaaac gcagatagcg
     3301 cgcaaccacg tcaaaagcaa caaaggtacg cccgtgaccg atatgacaga gatcgtaaac
     3361 ggtgattcca cacacataca tgccgacttc cccggcgtga ataggcttaa attcctcttt
     3421 ttggcgtgtc agagtattga agatttttag catcgaagat tccgtttaga catgtgtggg
     3481 taattgagtt gcgtataata cccatatttc ccgcgcgaat cagcatacat tgcgtgatga
     3541 tccgatcgtg cggttatgct ataacaccac cctatatatg acccgaactg ggttgaagca
     3601 ccaatcaaac ggaacaggat gcaaaaatgg ttactttcca caccaatcac ggcgatattg
     3661 tcatcaaaac ttttgacgat aaagcacctg aaacagttaa aaacttcctg gactactgcc
     3721 gcgaaggttt ttacaacaac accattttcc accgtgttat caacggcttt atgattcagg
     3781 gcggcggttt tgaaccgggc atgaaacaaa aagccaccaa agaaccgatc aaaaacgaag
     3841 ccaacaacgg tctgaaaaat acccgtggta cgctggcaat ggcccgtact caggctccgc
     3901 actctgcaac tgcacagttc ttcatcaacg tggttgataa cgacttcctg aacttctctg
     3961 gcgaaagcct gcaaggttgg ggctactgcg tgtttgctga agtggttgaa ggcatggacg
     4021 tggtagacaa aatcaaaggt gttgcaaccg gtcgtagcgg tatgcaccag gacgtgccaa
     4081 aagaagacgt tatcattgaa agcgtgaccg ttagcgagta atcgtggcga cactctttat
     4141 tgcagatctt catctctgcg tggaagaacc ggcgatcacc gccggttttc tgcgtttttt
     4201 agcgggggaa gctcgcaagg ccgacgcgct gtatattctt ggcgatctgt ttgaagcgtg
     4261 gattggcgac gacgatccca atccactcca tcgccagatg gcggcagcga tcaaagcggt
     4321 gtccgattcc ggcgttccct gttatttcat tcatggcaac cgtgattttc tgctcggcaa
     4381 acgctttgcc cgtgaaagtg gcatgacgtt attgccggaa gaaaaggtgc tcgaacttta
     4441 tggccgccgg gtgttgatta tgcatggcga cacgctgtgc accgatgacg cgggttatca
     4501 ggcttttcgc gccaaagtcc acaaaccctg gctgcaaatg ctattcctcg ccctgccgtt
     4561 gtttgtgcgc aaacgcattg ccgcgcgaat gcgagcgaac agtaaagaag ccaacagcag
     4621 taaatcgctg gcgatcatgg acgttaacca aaacgcggta gtcagtgcga tggaaaaaca
     4681 tcaggtgcaa tggctgatcc acggacatac ccatcgcccg gcggtacatg aacttatcgc
     4741 caaccagcaa cctgcttttc gcgtggtact cggtgcctgg catacggaag gttcgatggt
     4801 gaaagtcacg gcggatgacg ttgagctgat tcattttccg ttttaaaaaa cccgcaactt
     4861 tgctgatttc acagccacgc aaccgttttc cttgctctct ttccgtgcta ttctctgtgc
     4921 cctctaaagc cgagagttgt gcaccacagg agttttaaga cgcatgtctt cccgcaataa
     4981 tccggcgcgt gtcgccatcg tgatggggtc caaaagcgac tgggctacca tgcagttcgc
     5041 cgccgaaatc ttcgaaatcc tgaatgtccc gcaccacgtt gaagtggttt ctgctcaccg
     5101 cacccccgat aaactgttca gcttcgccga aagcgccgaa gagaacggtt atcaggtgat
     5161 tattgcgggc gcaggcggcg cagcgcacct gccaggcatg attgccgcca aaacgctggt
     5221 gccagtgctg ggcgtgccag tacagagcgc cgcactgagc ggtgtcgata gcctctactc
     5281 catcgtacaa atgccgcgcg gcattccagt gggtacgctg gcgattggta aagctggcgc
     5341 ggcaaacgcg gcgttactgg cagcacaaat tcttgcgact catgataaag aactgcacca
     5401 gcgtctgaat gactggcgca aagcccagac cgacgaagtg ctggaaaacc cggacccgcg
     5461 aggtgcggca tgaaacaggt ttgcgtcctc ggtaacgggc agttaggccg tatgctgcgt
     5521 caggcaggcg aaccgttagg cattgctgtc tggccggtcg ggctggacgc tgaaccggcg
     5581 gcggtgcctt ttcaacaaag cgtgattacc gctgagatcg aacgctggcc ggaaaccgca
     5641 ttaacccgcg agctggcgcg tcatccggcc tttgtgaacc gcgatgtgtt cccgattatt
     5701 gctgaccgtc tgactcagaa gcagcttttc gataagctcc acctgccgac cgcgccgtgg
     5761 cagttacttg ccgatcgcag cgagtggcct gcggtgtttg atcgtttaga tgaactggcg
     5821 attgttaagc gtcgcactgg tggctatgac ggtcgcggtc aatggcgttt acgcgcaaat
     5881 gaaaccgaac agttaccggc agagtgttac ggcgaatgta ttgtcgagca gggcattaac
     5941 ttctctggtg aagtgtcgct ggttggcgcg cgcggctttg atggcagcac cgtgttttat
     6001 ccgctgacgc ataacctgca tcaggacggt attttgcgca ccagcgtcgc ttttccgcag
     6061 gccaacgcac agcagcaggc gcaagccgaa gagatgctgt cggcgattat gcaggagctg
     6121 ggctatgtgg gcgtgatggc gatggagtgt tttgtcaccc cgcaaggtct gttgatcaac
     6181 gaactggcac cgcgtgtgca taacagcggt cactggacac aaaacggtgc cagcatcagc
     6241 cagtttgagc tgcatctgcg ggcgattacc gatctgccgt taccgcaacc agtagtgaat
     6301 aatccgtcgg tgatgatcaa tctgattggt agcgatgtga attatgactg gctgaaattg
     6361 ccgctggtgc atctgcactg gtacgacaaa gaagtccgtc cggggcgtaa agtggggcat
     6421 ctgaatttga cagacagcga cacatcgcgt ctgactgcga cgctggaagc cttaatcccg
     6481 ctgctgccgc cagagtatgc cagcggcgtg atttgggcgc agagtaagtt cagttaagca
     6541 ggtgctcatt tgattgccgg atgcggcgta aacgccttat ccggcttacc gctccggtac
     6601 ccattgtagg cctgataaga cgcgtcaagc gtcgcatcag gcatggtgca ccgattgccg
     6661 gatgctctca tcactacagc gaaatacagg tccctgcttc gcccgccagc gtctcttcaa
     6721 tccgcgataa cgccccaatc cacgcgggtt taccacggct tctgacataa ccactcaccg
     6781 ccgttacctt cggccccatc gaaccatcgg ctttggcaaa tggcgctaac tcatccggtg
     6841 tggcatggcg aatggcgcgt tgctgcggcg ttccccagtt ttcatatacc gcatcagcat
     6901 cggtgaggat caccagccca tcggcgttaa tcttctcggc aagcaaggca gcggcgagat
     6961 ctttatcaat caccgcttca ctccctgccc cgtcttccgt cacaggcaca ccgccgccgc
     7021 cactgcaaat caccacatgc ccctctttga gcaacaactc gatggcttcg ctgtcgagaa
     7081 ttttacgcgg ttgcggagac gccaccaccc ggcgcaaata tttaccatca cgtttcatct
     7141 gccagccgta agccgcttcc agtgcctctt gttcttctgg ctgataaacc ggaccaataa
     7201 atttctctgg ctgcaaaaac gccggatcat caggcgaaac ctcaatgcgc gtcagcaccg
     7261 tcgtaacagg cggcatctgc ggctgtgcgc tcaagctctg cgccagcata tagccaatca
     7321 tcccctggct ttccgccacc agcacatcca gcggatacgg ttctacctct ttccacgcca
     7381 gattctgtaa cgccagcagc cccacctgcg gcccgttgcc gtgaacaatc gccagccgat
     7441 aagaacgggc caggcgtgcc agcgcgggta cagcactggc gatattgcga tattgatttt
     7501 ccgccgtcag cgcctcaccg cgctggagta aggcgttgcc cccaagagct acaaccagtg
     7561 ttttcatggt tttccttgta ataattgttg gccaagccag aaccccagca gcgtatctgc
     7621 ccctgacgta tgccccagcg ccagcagcga atcaatcgca acagcggtac gtttcgggca
     7681 actcagagca tggacaaagt gcaggagtgg cgaggcgaaa tatccttgcg cggcataacg
     7741 taaataactg acgctcaccg ctgtggtaac gagttgaaga ttgtcggaat aggcaaaaaa
     7801 ctggcgacca gagcgcgcat ctaaagcgcc ataataccag gccgccagca gcattccgct
     7861 cagcgtgtca tcatgactcg gcgttaatcc ggggccttta cccagccagt gccgccagtc
     7921 ggtcttaacg ccattgagcg cggcctgaaa acagtgacga aactggcgta actcagcagg
     7981 cagcggatcg cctgccgcca acgccagtgg cccgaaaagc ccggtttcct ccgcgcgttg
     8041 catctatgca gctgcaagcg gttgaggatg cgcaggcggc gtaatacgca gcaaacaata
     8101 acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc ccttgagcca caacctgtgg
     8161 tcgttcattg ccgcataatc caccgcataa cgcatcgaat tgcgcgcggc gaagcaccca
     8221 tcctccgggg ccgaaaccac taccctgacg atgcagcgtt aacagttcgc cgctttccgt
     8281 catcaggtta atcgcccgtc gccacactcc cgctaaccgc caggactggc gataattcgg
     8341 tgcactacgg ctggcaagca gaggatggat gatcgtcatt gcgcgttcct caagcaatcc
     8401 ccatgctttc agccagtgct tccagcgcct gttcaaaaca cgccagcggt gcccgcacgg
     8461 tgcctgcgcc aatctgcccg atccccgcct ctttatgggc gataccggta ttgatgagtg
     8521 gcgtaatgcc ggtttctacc acgcgacgaa tgtccagtcc caggcacgcg ccctgaaaat
     8581 cccagcctgg gatctgcaac tgcatattgc gctcaaggta aatttccgcc atctcttcag
     8641 ataccgctct ggccgcttcc atgccacccg caccgacaaa gcgcgttacg ccaggcgctg
     8701 cgatcattgc cgcacctccg ataccaaagg tttcggtaat cgcgctatcg cccatatccg
     8761 ggttcgcctg ctcctgcgag aagccggtga aaaacagacc ttgcggagtg tttacaggcg
     8821 cagtaaacca gcgttcgccc agcccgctta cccgaatccc gaacatattg ccgttgcggg
     8881 tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc agcatccatc gccgccttgc
     8941 agtaagccat cgcgaggttg aggaagaact gatcggtcac gctgaggaaa tccatcactt
     9001 cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg tggggccagc gcacgcatta
     9061 acagtgcaga ggaagcaata ttgcgttgat ggaactcatc gcccatcgta atgccctgcg
     9121 ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc cagcgccgcg cttaatactg
     9181 gcattaacac atcgcgcatc cagcgatggc gagtcaggac atcttcgccg taagcgccaa
     9241 aacgcatcac tttgccgata ccttcgttga ggttgcagta cgcccggttg ccgtcggtca
     9301 cgttctcgac caccagcatc ggcatactgg cagaagtaat accgcccatt ggaccgacgg
     9361 cattcacatg gtgacaagga atgaagttca cttccccctg ctccagtatt gccagcgcct
     9421 gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc cacgcacgcc cctttcatgg
     9481 gtccggtcat ctcctgccag cgcattggcg gcccggcgtg aagcagtgtt ttgccctcgt
     9541 ttagttcgct gataagtgaa gaagccggtt gcacatccag ccagtgtgga cgagcgcgac
     9601 gaatttgttc gataaccgca gcattggctt gcgccactga tgtaaacatg ggaacccctt
     9661 attgcaaacg ttctaataaa cgagccagtt ttttattgcc gccggcgact ggcgaccatt
     9721 ggtaatgcac aaccggtttg ctggcgcttt gtagctccag cgcaaagctg cgtaatccga
     9781 tgttaatcac ggcgacgttt tccagtaatg acggtgtgtg ttgctgagtg gcaggcgaaa
     9841 gcggacgaat cagtgccgcc gccagcaagg tggcttccgg cagcgaactc actaccgcga
     9901 tccctgcatc ttccagcgtg gcgatttgct gcgagcggca ttgcgggtca cgttcagtgc
     9961 ctgtcaccgt ggcaatggca tacagtggtt gattgtctga acgcgcggca catgctgttt
    10021 gccaggcgct taccagcgag gcggcaggat cggcggtcgc accgaagcca atcacgacat
    10081 caagcagcaa tacgcgcact tgcggtttag cgccgagatc ggcaattaac tggttgcgta
    10141 aggctgggtc gatcatcgga tggggacgcc cgacggtgta gaaatcatcg ccgaggtcaa
    10201 taatctggtg accatcggcg tcgagcatca tgccgtgatg atgggtatcg tcggcttcca
    10261 cgccaaggtg tccagcaagt aatcccgccg cttcggcagc cagcgtaccg ccggtataca
    10321 aaccgcaaat aaatccgctg ctgacaggcg ctattgcgtt acgtcgcgcc gtgacgcgtg
    10381 aaagcaggca agccaggcgt gcggcctcat ccagcgagga ggcaaaccag acattctcgt
    10441 cgcgggccac cgccggggta taacctaaaa acagcgccac cgtcggtttg ccggttgctt
    10501 tcatggcatt aacaattttc agacgcacag cttcggcagg tggctttgaa acaaatgcca
    10561 gcacttcgct tttctcgtct gcactgagca tttccagcgc cgtcagcgca ctgatgccgc
    10621 ctacttcacg gctgaggtcg cgcccgccaa gaccaatcgc gtgagtaatt ccctcccctg
    10681 cctgcgcaat ctgcgaacac agttcctgaa tcccggtacc ggaagcgcca atgacgccaa
    10741 tgttgccttc cggcatcacg ttagcaaaag ccagcggcgt gccggcaatc atcgacgtac
    10801 cgcagtccgg ccccatcacc agcaagcctt tttctcgcgc gcgggtttta agttggattt
    10861 catcttccag cgtgacgtta tcggagaaca tcatcacgtt gaggttgcga tccagcgcct
    10921 gattcgccag ctccgccgca tactcgccag ccactgaaat cagcgccaga ttggcatcgg
    10981 ataatttctg acaggcactg tcccagcgac gcacctgcgt caaggactgg ctgctgcctg
    11041 acccctgcgc cagttgtttt agcgcctctt caagctgctg cataatcgcc tgcgcgatcc
    11101 ccgcatccgc cgcttcgcta cgaattgcca cgcaaatatc gttcggcgtg gcgttattaa
    11161 aatcgtcatg ccagaaacct gtggtatcta ataacgcttt attggcgggc gtgcccatca
    11221 ttacggaaac atcatcaaca ttttctgatt cgctgagttt tcgtgaaata atcattaaac
    11281 tgaccgaatc ctgaaaacac ccttttttaa taaaggcgtg gatcataaca actccttaaa
    11341 aattgcaccc atggcaattg cacgagcagt gttaatgtgg cgattacggt atcgatgaca
    11401 acgggtaaat aaggtgatga atatcacaca gggattatta acaaaagaat tcaaataacc
    11461 gccatgctat tacgtgttat tttttaataa gcgtggttgt tttaa
//
LOCUS       DAZKHC020000117        11431 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZKHC020000117 DAZKHC020000000
VERSION     DAZKHC020000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.117"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..80
                     /locus_tag="ACHOSM_RS19790"
                     /old_locus_tag="pgaptmp_003958"
     CDS             <1..80
                     /locus_tag="ACHOSM_RS19790"
                     /old_locus_tag="pgaptmp_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4560360.1"
                     /translation="GYDKIVVKYNNWITKDNGEFELAVH"
     gene            110..610
                     /locus_tag="ACHOSM_RS19795"
                     /old_locus_tag="pgaptmp_003959"
     CDS             110..610
                     /locus_tag="ACHOSM_RS19795"
                     /old_locus_tag="pgaptmp_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HGU4560361.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            702..3386
                     /locus_tag="ACHOSM_RS19800"
                     /old_locus_tag="pgaptmp_003960"
     CDS             702..3386
                     /locus_tag="ACHOSM_RS19800"
                     /old_locus_tag="pgaptmp_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HGU4560362.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGGGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVIVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLLLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            3383..4468
                     /locus_tag="ACHOSM_RS19805"
                     /old_locus_tag="pgaptmp_003961"
     CDS             3383..4468
                     /locus_tag="ACHOSM_RS19805"
                     /old_locus_tag="pgaptmp_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HGU4560363.1"
                     /translation="MRNRLIAMILCLFDMAASVQAAPNVTAEVTYDLASGRANYYFWN
                     REDPPAVNYNTTWANYKCDFPENQQSCTSNGNSSSVVVYLTEKRSGMRWPVKLIGYIN
                     AEVWQDGPCKGWSKQLALNNGSGYYCMGDDHVQYLAQAKPVTLYLEQAEMKKLPIGGI
                     WEGSVKLRFTTPGTDYTANITLNVLDPNHIDIFFPEFAHATPRVQLDLHPTGSVNGSN
                     YAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGERI
                     DYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVDK
                     FSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4558..5856)
                     /gene="exuT"
                     /locus_tag="ACHOSM_RS19810"
                     /old_locus_tag="pgaptmp_003962"
     CDS             complement(4558..5856)
                     /gene="exuT"
                     /locus_tag="ACHOSM_RS19810"
                     /old_locus_tag="pgaptmp_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HGU4560364.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            6339..7751
                     /gene="uxaC"
                     /locus_tag="ACHOSM_RS19815"
                     /old_locus_tag="pgaptmp_003963"
     CDS             6339..7751
                     /gene="uxaC"
                     /locus_tag="ACHOSM_RS19815"
                     /old_locus_tag="pgaptmp_003963"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HGU4560365.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7766..9253
                     /gene="uxaA"
                     /locus_tag="ACHOSM_RS19820"
                     /old_locus_tag="pgaptmp_003964"
     CDS             7766..9253
                     /gene="uxaA"
                     /locus_tag="ACHOSM_RS19820"
                     /old_locus_tag="pgaptmp_003964"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HGU4560366.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9336..9887
                     /gene="ygjV"
                     /locus_tag="ACHOSM_RS19825"
                     /old_locus_tag="pgaptmp_003965"
     CDS             9336..9887
                     /gene="ygjV"
                     /locus_tag="ACHOSM_RS19825"
                     /old_locus_tag="pgaptmp_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HGU4560367.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9892..11136)
                     /gene="sstT"
                     /locus_tag="ACHOSM_RS19830"
                     /old_locus_tag="pgaptmp_003966"
     CDS             complement(9892..11136)
                     /gene="sstT"
                     /locus_tag="ACHOSM_RS19830"
                     /old_locus_tag="pgaptmp_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HGU4560368.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         3037 a         2760 c         2939 g         2695 t
ORIGIN      
        1 ctggctacga caaaatcgtt gtgaaatata ataactggat caccaaagat aatggcgagt
       61 ttgaactggc ggtccattaa ttatcattgt aataaacgaa ggaatagtaa tgaaaaaggt
      121 atttgcaaaa tctcttctgg tcgcagcgat gttttctgtt gcaggctccg cattggcagt
      181 acaaaaggat attactgtaa cggccaacgt tgatgccgct ctggatatga cacagaccga
      241 taacaccgcg ttgccgaaag cagtagaaat gcaatatctg ccggggcagg gtctgcaatc
      301 ttaccagttg atgaccaaaa tctggtctaa cgacgttacc aaagatgtaa aaatgcagtt
      361 agtttccccg gcgcaactgg ttcaaagcct ggatgctagc aagattgttc cgctgacagt
      421 aacctggggc ggtgaagaaa ttaaagctga tgcggcaacc acttttaccg caaccaaaat
      481 ctttgcttcc gatgcgttaa ctaacggttc tctggctaaa aacctgatgt ttgctcaaac
      541 caccaaaggt gttctggaga caggcatcta ccgtggtgta gtaagcattt atctgtctca
      601 ggatatctaa tccagataac agaattaaca cgtaacaaag ggaggggtag ccctccctta
      661 ttttaactgt tttagtattt gttatggatt tcaggattgc catggataaa aaattactgg
      721 ctcttttgat cctggcgagt ctcagcccgg cagaggcgac attaaccaaa attcccgcag
      781 ggtttgaggt tattgctcag ggacagcagg agtatatcga ggtttatttt tcagggaaaa
      841 gtctcggtaa atattatgca atggttaatc ttgataccgt aacatttctt gatccagcaa
      901 gtctatataa caagctggaa ctggatgtag acgatcagaa aatcgcgcat atagtgaaag
      961 aaaaattatc gcagccgcta gctcgccacg gtgaattggc ttgcggttat gtacgtactg
     1021 actcaggggg tggttttctg aataccgata cgctggaaat aatctataat gatgaagaaa
     1081 gttcggcaac gttgtttatt aatccgcaat ggaattcagc tttcgatgcg aagtcattat
     1141 atttaaatcc agacaaaaat actgttaatg cttttataca tcagcaagac atcaatgttc
     1201 tggcacagga tgattaccaa tcgttgtcta ttcagggaaa cggtgcgctg ggaataacag
     1261 aaaatagtta tattggtgca cactggaatt tcaacggtta tgatgcagat gatgtcagtg
     1321 acagtaatgc tgacgtcagc gatctctatt atcgttatga ttttttacgt cgttattatg
     1381 tgcaggcggg tcgcatggac aaccgcacac tatttaatgc acaaggcggg aactttacct
     1441 ttaactttct gccactcggt gcaattgatg ggatgcgtat cgggtcgaca ctgagctatt
     1501 taaaccaggc gcaaagccag caaggaaccc cagtaatggt tctgctttcg cgcaattctc
     1561 gtgttgacgc ttatcgtaat gagcaacttt tgggatcgtt ttatctcaat agtggttcgc
     1621 aatttattga taccagttcc tttccgccag gcagctacag cgtggcatta aaagtctacg
     1681 aaaataacca actcacccgc accgagcttg tgccgtttac caaaaccggc ggtctgactg
     1741 acggaaatgc gcaatggttc ttacaggcag gtaaaactac atcacaggtt tctgatgatg
     1801 aaagttcagc ttatcagcta ggagtacgcc tgccattaca tccgcaatat gagctctacg
     1861 cagggctggc gaatgccgat gatgtgagtg ctttcgagtt aggtaataac tggacggcag
     1921 atttaggcgg ggtggggaat cttgcaatca gcgccagcgt gttccgtaac gatgacggcg
     1981 gcaaaggtga tatgcaacag gccaactgga gtaatccggg atggccgacg ttgggctttt
     2041 atcggaccaa ctctgacggc gatgcttgta caaccgacag cagagagagc tataacgcct
     2101 taagctgtta tgaaagtatt tccgcgacgg tttcacagaa ttttgtcggc tggaatatga
     2161 tgctgggtta tacccgcaca caaaataaca ctgatgatag tttgcgttgg gataaacagc
     2221 agagctttga aaataactat cttcgccaga caacagcgca aagtatctct gaaactgtac
     2281 aacttagcgc ttcccgcgct tttgtgatgc gtgactggat tttgagtacg tcagtcggtg
     2341 ttttccatcg taatgacaac ggtggcgata acgacgacaa cggcttgtac ttatcgtttt
     2401 cgttatctga cacgccaacg atggatagca ataacaacag ccattcaaca aatgtgtcta
     2461 cggattatcg ttatagcgaa caggatggcg atcaaacgtc atggcagtta tcgcatacct
     2521 tttataacga ttcattcagc cataaagaac ttggcgtaat cgtcggaggc ctgaacaccg
     2581 ataccataaa cagcgcggtt aacgggcgtt gggatgggca atacggaaat gtctacgcta
     2641 ccgtatctga tagttatgac cgtaagaatc atgatcatct ttcggccttt acgggcactt
     2701 atagctctac gctggctgtc agtcgctatg gcgttaattt gggagccagt ggtacagacg
     2761 atttgctggg tgcggtactg gtggatgtga aaggcttctc tgaacaggat gaagagagtc
     2821 aggatctgca actcgaagcg cgggtggcag gcagccgaac tttgctgctt ggtcaaagtg
     2881 acagtgtgtt gttcccttat cctggatttc agtctggttt tgttgaggtt aacgacagta
     2941 gccagggcaa tcagcagggg acaacaaaca tcattaacgg tgcagggaat cgtgaattaa
     3001 tgttgttgcc tggcaagctg cgctatcgcg aagtgtctgc cagctttaac tacaactata
     3061 tcggtcgttt attactgccg gcagcggtga aaaaattccc gattgtgggg ctgaacagcg
     3121 ccatgttact ggtagctgaa gatggcggat ttacacttga aattaacggc agcgaaaaag
     3181 agctctatct gctttccggg cagcaattcc ttaagtgtcc actgagtgtt gtaaagaaac
     3241 gcgccagcat tcgttacagc ggagatgtta cttgtagtgt ggtgacttat tcacaattac
     3301 cggagtcgat tcaggttcag gcacagttga aacagcctaa attacgtgga aacgttcaga
     3361 cggcgcaaag ggaggttgca ccatgagaaa tcgattgatt gccatgattt tatgcctgtt
     3421 tgacatggcc gcgagtgttc aggcggcacc taatgtgacg gctgaagtta cttatgattt
     3481 ggcgtctggc agggcaaact actatttctg gaatcgcgaa gatccaccag cggtaaatta
     3541 caatactacc tgggcaaact ataagtgtga tttcccggag aatcaacaga gctgcacatc
     3601 taatggcaat agttcatcgg tagtggttta tttaacagaa aaacgcagcg gtatgcgctg
     3661 gcctgtcaag ttaattggct acatcaatgc agaagtctgg caagatgggc catgtaaagg
     3721 atggagcaag caattagcat taaacaatgg ttcgggttat tactgcatgg gggatgatca
     3781 tgtccaatat cttgctcagg caaagccagt gacactttat cttgaacaag cagaaatgaa
     3841 aaagttgccg atcggtggta tttgggaagg ttctgtgaaa ttgcgtttta ccaccccagg
     3901 aacagactat accgccaata tcacgcttaa cgttcttgac cctaaccata ttgatatttt
     3961 cttcccggag ttcgcccacg ccacgccacg ggtgcagtta gacttgcatc caacaggcag
     4021 cgttaacggc agcaactacg cgcaagatct gaccatgttg gacatgtgcc tgtacgatgg
     4081 ctttaacggt aatgccatca gttatgaaat catgcttaaa gatgaagggc gaccagctgc
     4141 agggcgcaga gacggttact tctctatcta tcgtcaggga gggaccacca ccgacgaggg
     4201 agaacgcatt gattaccggg tcaaaatgta caacccggaa accggtgggc aaattgatgt
     4261 gcgcaataat gaaaatatgg tctggaacag cattaacctg aaacgtgtgc gtccggtggt
     4321 actgcccggt attcgctatg ccgtgatgtg tgtgccgaca cccttgactc tggcagtcga
     4381 taaattcagc gtgatggata aacaggccgg gtactacatg ggcaaattgt cagtaatctt
     4441 tacgccttcc ttgccaacca tcaattgacc ccgcagacca gaaaaaacta aacaacgaag
     4501 ccgcctgata aggcggcttc gtcattacaa cttacgttaa acgttaaacg ttaaacgtta
     4561 atgttgcggt gcgggatcgt tatgtgattc ctgcgctacc tcaatcgccg gtttgttctg
     4621 caacacggtc cagataacca gcgcacctaa caggtcgaac actgccagta ctgcgaacag
     4681 cgggctgaag ccgatggtgt cagccagtgc accgacaacc agcgcaaaca gcgtacttgc
     4741 cagccatgcg gacatcccgg ttaaaccgtt cgccgtcgcc acttcgttac gaccgaacac
     4801 gtcagaagag agcgtaatca gcgcgccaga cagtgcctgg tgggcaaaac caccgataca
     4861 cagcagcata attgcgacat acgggttggt gaacagaccg atcatacccg ggccaatcat
     4921 cagcacagca cccagcgtta cgaccatctt acgggaaacg atcaggttca caccaaacca
     4981 acgctggaac agcggcggca ggtaaccacc gaggatacaa ccgaggtcag caaacagcat
     5041 cggcatccaa gcgaacatcg cgatctcttt caggttaaag ccgtaaactt taaacatgaa
     5101 cagcgggatc cacgcgttaa aagtacccca ggccggttct gccaggaaac gcggcagtgc
     5161 gataccccag aactgacggt tacgcaggat ctgaccaacg gacattttct tcgccgtgct
     5221 cacctggtgc tgggcttcct gaccattaat aatatagtcg cgttcttcat cggtcagatg
     5281 cttctggtcg cgcggatgtt tatagaaaat cagccatgcc atcgcccaga taaagctcaa
     5341 tgcaccggag atgataaatg ccatctgcca gctgtgcatt acgattgccc ataccaccag
     5401 cggcggcgca atcatcgcac caatcgaaga acctacgtta aagtaaccta ccgcgatgga
     5461 acgctctttc gccggaaacc attcggagct ggctttcaga cccgccggga tcatcgctgc
     5521 ttccgcggca ccgactgcac cacgagcaat cgccaggcca ccccagctac cagccagcgc
     5581 ggttgcacca cagaatacgg cccacagtac agcaaacatt gcataaccga ttttcgtacc
     5641 cagcacatcc agtacatagc ccgctaccgg ttgcatgacc gtataagcag cagaataggc
     5701 tgcgatgata taggaatact gttgggtgga gatgtttaac tcttccatca gagttggcgc
     5761 agctgccgcc acagtgttac gcgtcaggta accaagcacg gtgccaagcg tcaccagtgc
     5821 gatcatatac caacgtaacc ctttaatttt acgcatgtaa aacctcatcg ttttgttata
     5881 tccgcaggaa gcgcggccat catcccgcca gaggctgaag atgttagtta cgaccgggta
     5941 accgaaaaaa aaccggcacg ccccgaacgt tgccatgaaa gtttcgtgct aattcggctt
     6001 ccgtaccggt taacccgatt catctaacaa atgaatgaat cggcaatttg cattccgtcg
     6061 acttatgatt tgcgacggca gaaagataac ttgtcataca actttaaaag gtgagagcca
     6121 tcacaaatgt gcgaatattt gtagggacat tacctgacga cagcgaggcc agtactggcg
     6181 cggcctgtag cgagatttac cactttgaga gtaatttttt taactacgtt tattgatcta
     6241 actcacgaaa atatcttcgg actctggaaa ttggtgtgat aactttgtca gcatcgcacc
     6301 ataagcaagc tagctcactc gttgagagga agacgaaaat gactccgttt atgactgaag
     6361 atttcctgtt agataccgaa tttgcccgcc gtctgtatca cgactacgca aaagaccagc
     6421 cgattttcga ttaccattgc catttgccgc cgcagcagat tgcggaagac tatcgtttta
     6481 aaaacctgta tgacatctgg ctgaaaggcg atcactacaa atggcgcgct atgcgtacca
     6541 acggtgtggc cgagcgtctg tgtaccggtg atgcatctga ccgtgaaaaa tttgacgcct
     6601 gggcggcgac tgttccgcac actatcggca acccgttata ccactggacg caccttgaac
     6661 tgcgtcgtcc gtttggtatc actggcaaat tgctttctcc gtcaactgcc gatgaaatct
     6721 ggaacgaatg taacgaactg ctggcgcagg ataacttctc cgcgcgcggc atcatgcagc
     6781 agatgaacgt gaaaatggtc ggcaccaccg atgacccgat cgattctctg gagcatcacg
     6841 cagagatcgc caaagacggc tctttcacca tcaaagtgct gccgagctgg cgtccggaca
     6901 aagccttcaa catcgaacag gcgaccttta acgactacat ggcgaagctg ggcgaagttt
     6961 ccgataccga cattcgccgc tttgctgacc tgcaaactgc cttgaccaaa cgtctggatc
     7021 acttcgccgc tcacggctgt aaagtgtctg accatgcgct ggacgtggtg atgtttgctg
     7081 aagcgaacga agcggaactg gacagcattc tggcgcgccg tctggctggc gaaaccctga
     7141 gcgagcacga agtggcacag ttcaaaactg cggtactggt gttccttggt gccgaatatg
     7201 cacgtcgcgg ctgggtacag cagtaccaca ttggcgcgct gcgtaataac aacctgcgtc
     7261 agttcaaact gctggggccg gatgtaggct ttgactccat caacgaccgt ccgatggcgg
     7321 aagagttgtc taagctgctg agcaagcaga acgaagaaaa cctgctgccg aaaaccattc
     7381 tctactgcct gaacccgcgc gataacgaag tgctgggcac catgatcggt aacttccagg
     7441 gcgaaggtat gccgggcaaa atgcagttcg gttccggctg gtggtttaac gatcagaaag
     7501 acggtatgga acgtcagatg acccaactgg cgcagctcgg tctgctgagc cgctttgtcg
     7561 gtatgctgac tgacagccgt agcttcctgt catacacccg tcacgaatac ttccgccgca
     7621 ttctgtgcca gatgatcggt cgctgggtgg aagcgggcga agcaccggcg gacatcaacc
     7681 tactgggcga gatggtgaaa aatatttgct ttaacaatgc gcgtgactac ttcgccattg
     7741 aactgaacta aggtctgggg ttgatatgca atacatcaag atccatgcgc tggataacgt
     7801 cgcggtcgct ttagcagatt tggctgaagg cacagaagtc agtgtcgata accagactgt
     7861 tacgctgcgc caggatgttg ctcgtggaca taaatttgcg ttaacggata tcgcaaaagg
     7921 ggccaacgtc atcaaatatg gcctgccgat tggttatgca ttggcggata ttgcggcggg
     7981 ggaacacgtc cacgcccaca atacgcgcac gaatctgagc gatctggatc agtatcgcta
     8041 tcaacctgat tttcaggatc tgcctgcgca agcggcagat cgtgaagtgc agatctatcg
     8101 tcgcgctaac ggcgatgtcg gggtgcgtaa tgagctgtgg atcctgccaa ccgtgggctg
     8161 tgtcaacggc atcgcgcggc aaatccagaa ccgtttcctg aaagagacca acaacgccga
     8221 aggtaccgac ggcgtgttcc tcttcagcca cacctacggc tgctcacaac tgggcgacga
     8281 tcacatcaac acccgcacca tgctgcaaaa catggtgcgc cacccgaacg cgggcgcagt
     8341 gctggtgatt ggtctgggct gtgaaaacaa ccaggttgcc gcattccgtg aaacgttggg
     8401 cgatatcgat cctgaacgcg ttcatttcat gatctgccaa cagcaggatg atgagatcga
     8461 agctgggatc gagcatttgc atcagctgta taacgtgatg cgcaacgata aacgcgagcc
     8521 aggcaaactc agcgaactga agtttggtct ggagtgcggt ggttctgacg gtctttctgg
     8581 tattactgct aacccgatgc tggggcgttt ctctgactac gtgattgcta acggcggtac
     8641 taccgtactg accgaagtgc cggagatgtt tggcgcagag cagttgctga tggaccattg
     8701 ccgcgacgaa gcaacgtttg aaaaactggt caccatggtc aacgacttta aacagtactt
     8761 tattgcccat gatcagccga tctacgaaaa cccatcaccg gggaacaaag cgggcggtat
     8821 caccacgctg gaagacaaat cacttggctg tacccagaaa gcgggttcca gtgtcgtggt
     8881 tgacgtgctg cgttacggcg agcgtctgaa aacgccaggg ctgaacttgt taagtgcgcc
     8941 gggtaacgat gccgtagcga ccagcgccct ggcgggtgcg ggctgccata tggtgctgtt
     9001 cagtactggt cgcggcacgc catacggtgg atttgtgccg acggtgaaaa tcgccaccaa
     9061 cagtgaactg gcggcgaaga aaaaacactg gatcgacttt gacgcgggtc agctgatcca
     9121 cggtaaagcg atgccgcagt tgctggaaga atttatcgac accatcgttg agtttgccaa
     9181 cggtaaacaa acctgcaacg agcgtaacga cttccgcgaa ctggcgatct tcaaaagcgg
     9241 cgtaacgcta taaagtcgaa atgagttgtt aataaaaaac ggcgtttcat aatgtgaagc
     9301 gccgtttgct tttccatacc tgataaggat ttatcatgac cgcgtattgg ctggcccagg
     9361 gcgtgggtgt catcgccttt ctgattggta tcacaacatt tttcaatcgt gacgaacgtc
     9421 gcttcaaaaa gcagctttcg gtctatagcg ccgttattgg cgtacatttt tttcttctgg
     9481 gcacctatcc cgctggtgcc agtgccatcc ttaatgccat tcgtacattg attaccttac
     9541 gcacgcgcag cttatgggta atggcgattt ttattgtgct gactggcgga attggcctcg
     9601 cgaagttcca tcatcctgtc gaactattgc cggttatcgg tacgattgtc agtacctggg
     9661 cgctgttccg ctgtaaaggg ctcaccatgc gctgcgtgat gtggttttca acgtgttgct
     9721 gggtgattca caacttctgg gcggggtcga taggcggcac aatgattgag ggtagttttc
     9781 tgcttatgaa tggcctgaat atcattcgtt tctggcggat gcagaaaagg ggaattgatc
     9841 cgtttaaagt agagaaaacc cctcccgccg tagacgaaag gggttaaaca attaattacg
     9901 cagggcgcta tttgccagac gatcgtcttc cgcctggcaa gctgccgcag tgaacagcac
     9961 gtcagttgaa gagttcagcg cggtttcgca agagtcctgc aatacgccga taataaagcc
    10021 gacggcaacc acctgcatgg cgatatcgtt cgaaataccg aacatattac aggccagtgg
    10081 gatcagcagc agagaacccc ccgccacgcc ggatgcgcca caggcacaca gagaagccac
    10141 tacgctcaac agcagcgctg tgggcagatc gaccggaata cccagcgtat taaccgcagc
    10201 cagcgtcaac acggtaatgg tgattgctgc gcccgccata ttgatggtgg ctcccagcgg
    10261 aatagaaacg gaataggtat cgcgatccag attcagcttt tcacacagcg ccatattcac
    10321 cggaatgttc gctgcagagc tgcgggtgaa gaaggcatac acaccgcttt cgcgcaggca
    10381 cagcagcacc agcgggaacg ggttacgacg aattttccac cacaccagca atgggttaac
    10441 caccagcgcc accagtaaca tacagccaac cagcacgacc agcagttgcg cgtagcccca
    10501 cagtgtggag aaaccggtgg ttgccagggt agaagaaacc agcccaaaaa taccaatcgg
    10561 tgcgaagcga atgaccagtt tcaccataaa ggtaacggca ttcgacatat cgttaaccag
    10621 gtttttggtg gtctcgttac cgtgacgcag tgcgaaaccg aggccaattg cccacaccag
    10681 gatcccgatg tagttacctt tcagcagcgc gtcgatgggg ttggaaacca tgctcattac
    10741 cagcccgcgc atcacttcta caatgcctga cggtggcgaa atatcacccg cgctactgga
    10801 taagtgcagg gtagaaggga aggcgaagct gaaaacgact gcggccagag cagcagagaa
    10861 ggtacccagc agatagagga acaaaatagg gcggatattg gttttctgcc cgtgctggtg
    10921 gttagcaata gatgccatca ccagcatcaa caccaggatg ggggcaacgg ctttcagtgc
    10981 gccgacgaac aaagtaccta acagaccaac agcttccgcc gcgggttttg agatccatgc
    11041 cagaagaatc cccagaacaa ggccgaccag gatttgtttt accaggctgc catgagccag
    11101 acgccggaat agccccggtg aacgttgcgt agtcattttt cgatcctttc attgtgttgt
    11161 cgcacatccc tggtgcgttc tgtcgacgca tctttcagga tgtaacaaag tgtttgcaag
    11221 gtcgagtata aggaaagtta cagcttcggg aagcgaaaaa tgctggattt taaagagagg
    11281 tcatattttg caacttaatt gttacatttg tttgacataa acagagggaa ggactggcta
    11341 ctaaggctga ttagataaca tatgggcaaa actatttgga atatgagaac ggttgcgctc
    11401 ctttaatgtg ctatgccata taaagtcact g
//
LOCUS       DAZKHC020000118        11242 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZKHC020000118 DAZKHC020000000
VERSION     DAZKHC020000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.118"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..713
                     /gene="ydeO"
                     /locus_tag="ACHOSM_RS19835"
                     /old_locus_tag="pgaptmp_003967"
     CDS             3..713
                     /gene="ydeO"
                     /locus_tag="ACHOSM_RS19835"
                     /old_locus_tag="pgaptmp_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HGU4560369.1"
                     /translation="MLDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKKY
                     LQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFA
                     ACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTT
                     FSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYR
                     CQSHTGMNTGNTMSALAI"
     gene            1115..2797
                     /gene="ydeN"
                     /locus_tag="ACHOSM_RS19840"
                     /old_locus_tag="pgaptmp_003968"
     CDS             1115..2797
                     /gene="ydeN"
                     /locus_tag="ACHOSM_RS19840"
                     /old_locus_tag="pgaptmp_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4560370.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKRSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPAALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2849..4006
                     /gene="ydeM"
                     /locus_tag="ACHOSM_RS19845"
                     /old_locus_tag="pgaptmp_003969"
     CDS             2849..4006
                     /gene="ydeM"
                     /locus_tag="ACHOSM_RS19845"
                     /old_locus_tag="pgaptmp_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU4560371.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCLKCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3972..4094)
                     /locus_tag="ACHOSM_RS19850"
                     /old_locus_tag="pgaptmp_003970"
     CDS             complement(3972..4094)
                     /locus_tag="ACHOSM_RS19850"
                     /old_locus_tag="pgaptmp_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560372.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            4297..5982
                     /gene="yddA"
                     /locus_tag="ACHOSM_RS19855"
                     /old_locus_tag="pgaptmp_003971"
     CDS             4297..5982
                     /gene="yddA"
                     /locus_tag="ACHOSM_RS19855"
                     /old_locus_tag="pgaptmp_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HGU4560373.1"
                     /translation="MITIPITLRMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFDALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVMLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWNRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     CKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
     gene            6020..8392
                     /gene="yddB"
                     /locus_tag="ACHOSM_RS19860"
                     /old_locus_tag="pgaptmp_003972"
     CDS             6020..8392
                     /gene="yddB"
                     /locus_tag="ACHOSM_RS19860"
                     /old_locus_tag="pgaptmp_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HGU4560374.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTWNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPIEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGRLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFANQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            8449..11232
                     /gene="pqqL"
                     /locus_tag="ACHOSM_RS19865"
                     /old_locus_tag="pgaptmp_003973"
     CDS             8449..11232
                     /gene="pqqL"
                     /locus_tag="ACHOSM_RS19865"
                     /old_locus_tag="pgaptmp_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU4560375.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3317 a         2459 c         2429 g         3037 t
ORIGIN      
        1 acattttaga taaagattac gccttctctg acggcgagat cctgatggta gataacgctg
       61 ttcgtacaca ttttgaacct tatgagcggc attttaaaga aatcggattt aatgaaaata
      121 ccattaaaaa atatctacaa tgcactaaca tccagacagt gacggtgcct gttcctgcga
      181 agtttttacg tgcttcaaat gtaccgactg gattgcttaa tgaaatgatt gcttatctca
      241 actcggaaga acgcaatcat cataattttt cagaactttt gcttttttct tgcctgtcta
      301 tttttgccgc atgcaaaggt ttcattacac tattaactaa cggtgtgcta tccgtttccg
      361 ggaaagtgag aaatattgtc aacatgaagc tggcgcaccc atggaagctg aaagatattt
      421 gtgactgcct gtacatcagt gaaagtctgt tgaagaaaaa acttaagcaa gagcaaacga
      481 cattctcaca gattctttta gatgccagaa tgcagcacgc aaaaaatttg atacgcgtag
      541 aaggttcagt caataaaatt gccgaacaat gtggttatgc cagtacatct tattttattt
      601 atgcgttccg caaacatttc ggcaacagtc cgaagagagt ttctaaggag taccgttgtc
      661 aaagtcacac gggtatgaat acgggcaaca cgatgagtgc tttagctatt tgattatttg
      721 ctaacgagta gtcaaccacg cacgctacgt aagaattata tggggcagcc attccctgcc
      781 ccgcgttgtt tttaggcgat atatttattg aaataaataa gtgacatcca tcacatattt
      841 atgcacttgc ataacctgtt gcatgattat ttatgatctc aattcggcat tttgtcagta
      901 aaatgcaata atttattaaa tatcaataaa ttagttgttt atcggcgaga aattacttaa
      961 tagaacagaa agtaatgtca acgctttatg gactgttatt tccctttttt tagctaaatc
     1021 tgctatctct ttatgtgact aacttcactt acatccactt atttctcctc gtaaaattac
     1081 ttcggaatta agtacaataa gaagaggaac atttatgaag tctgcattaa agaaaagtgt
     1141 cgtaagtacc tcgatatctt tgatactggc atctggtatg gctgcatttg ctgctcatgc
     1201 ggcagatgat gtaaagctga aagcaaccaa aacaaacgtt gctttctcag actttacgcc
     1261 gactgaatac agtaccaaag gaaagccaaa tattatcgta ctaaccatgg atgatcttgg
     1321 ttatggacaa cttccttttg ataagagatc ttttgaccca aaaacaatgg aaaatcgtga
     1381 agttgtcgat acctacaaaa tagggataga taaagccatt gaagctgcac aaaaatcaac
     1441 gccgacgctc ctttcattaa tggatgaagg cgtacggttt actaacggct atgtggcaca
     1501 cggtgtttcc ggcccctccc gtgccgcaat aatgaccggt cgagctcccg cccgctttgg
     1561 tgtctattcc aataccgatg ctcaggatgg tattccgcta acagaaactt tcttgcctga
     1621 attattccag aatcatggtt attacactgc agcagtaggt aaatggcact tgtcaaaaat
     1681 cagtaatgtg ccggtaccgg aagataaaca aacgcgtgac tatcatgaca acttcaccac
     1741 attttctgcg gaagaatggc aacctcaaaa ccgtggcttt gattacttta tgggattcca
     1801 cgctgcagga acggcatatt acaactcccc ttcactgttc aaaaatcgtg aacgtgtccc
     1861 cgcaaaaggt tatatcagcg atcagttaac cgatgaggca attggcgttg ttgatcgtgc
     1921 caaaacactt gaccagcctt ttatgcttta cctggcttat aatgctccgc acctgccaaa
     1981 tgataatcct gcaccggatc aatatcagaa gcaatttaat accggtagtc aaacagcaga
     2041 taactactac gcttccgttt attctgttga tcagggtgta aaacgcattc tcgaacaact
     2101 gaagaaaaac ggacagtatg acaatacaat tattctcttt acctccgata atggtgcggt
     2161 tatcgatggt cctctgccgc tgaacggggc gcaaaaaggc tataagagtc agacctatcc
     2221 tggcggtact cacaccccaa tgtttatgtg gtggaaagga aaacttcaac ccggtaatta
     2281 tgacaagctg atttccgcaa tggatttcta cccggcagct cttgatgcag ccgatatcag
     2341 cattccaaaa gaccttaagc tggatggcgt ttccttgctg ccctggttgc aagataagaa
     2401 acaaggcgag ccacataaaa atctgacctg gataacctct tattctcact ggtttgacga
     2461 ggaaaatatt ccattctggg ataattacca caaatttgtt cgccatcagt cagacgatta
     2521 cccgcataac cccaacactg aggacttaag ccaattctct tatacggtga gaaataacga
     2581 ttattcgctt gtctatacag tagaaaacaa tcagttaggt ctctacaaac tgacggatct
     2641 acagcaaaaa gataaccttg ccgccgccaa tccgcaggtc gttaaagaga tgcaaggcgt
     2701 ggtaagagag tttatcgaca gcagccagcc accgcttagc gaagtaaatc aggagaagtt
     2761 taacaatatc aagaaagcac taagcgaagc gaaataacta aaccttcatg cggcggaatt
     2821 ttccgccgcc ttattgagcg agatagcgat gcacgttaca gccaagccct ccagttttca
     2881 atgtaatctc aaatgtgatt actgttttta ccttgaaaaa gagtcgcagt ttactcatga
     2941 aaaatggatg gatgacagca ctctgaaaga gttcatcaaa caatatatcg cagcgtctgg
     3001 caatcaggtc tattttacct ggcaaggcgg tgaacccact ctggctggcc tggatttttt
     3061 ccgtaaagtt attcactatc aacaacgcta tgcaggccaa aaacgtattt ttaatgcatt
     3121 acaaacgaat ggcattttat tgaataatga atggtgtgct tttctcaaag aacatgagtt
     3181 tctggttggt atctcgatcg atggccccca ggagctacat gaccgttaca gacgcagtaa
     3241 ttcaggtaac ggtacttttg caaaagtgat agcagccatc gagcgtctga aatcatatca
     3301 agttgagttt aatacgttaa ccgtcattaa taacgttaat gtccattacc ctcttgaggt
     3361 ttatcatttt ttaaaatcta tcggcagtaa acatatgcaa tttatcgaat tgctcgaaac
     3421 cgggacgccg aatattgatt tcagtggtca tagtgagaac acattccgta tcattgattt
     3481 ttctgtgcct cccacggctt atggcaagtt tatgtcaacc atttttatgc aatgggttaa
     3541 aaacgatgtg ggtgaaattt tcatccgtca gtttgaaagc tttgtcagcc gttttttagg
     3601 gaatgggcat accagttgta ttttccagga gtcctgcaag gataatctgg ttgttgaaag
     3661 taatggagat atttatgaat gcgaccattt tgtctatcca cagtacaaaa ttggaaacat
     3721 taataaatct gaactcaaaa cgatgaacag tgtacaactg acagcgcaaa aaaaacggat
     3781 ttcagcgaaa tgtctgaaat gtgcatataa acctatctgc aatggcggtt gtcctaagca
     3841 tcgtattact aaagtaaaca atgagactgt ttcttatttt tgcgaaggtt ataaaatcct
     3901 tttttcaacc atggtacctt atatgaacgc catggtggag ttagctaaga acagagtacc
     3961 gctttaccac attatggatg ttgcaaaaca aatggagaat aattaataac gctctctttt
     4021 aactccattc ttagcctcct tcttatgacc ttaacaaggc acccgacata aattcctgtc
     4081 gggtgtttcg tcatcatcat cttcccttgc gaaaatcctg gcttacgtgg tatgcatcag
     4141 ggcattagca atatccttga ttatcaatag cataaccata accactgaaa atgaaatatt
     4201 cctttatatt tcatattgtt aaatgatact taaataaatt tcatcagtaa atcattgcca
     4261 tagataatca ttggatggca caatcaattt tcgctaatga taaccattcc cattacgctt
     4321 cgtatgttga tagcaaagta tctctgtctg ttgaagccct tttggctgcg taagaataat
     4381 aaaacttcag tgctgttaat cattattatc ctcgccatga tccttggcgt ggttaaaatt
     4441 caggtctggt taaacgactg gaataatgat tttttcgatg cgctaagcca gaaagaaacc
     4501 gataagcttt ggcagcttgt cctttggttc cctgccctgc tggggatctt tgtattaata
     4561 tccgtgaaca aaacatggtt aattaaactg ctgactattc gctggcgcga atggttaaca
     4621 gattactacc ttaatcgctg gtttgcagat aaaaactact acttcacgca gatctacggc
     4681 gagcataaaa atacagataa ccctgaccaa cgtatcgctg aagacattct cttactaatc
     4741 agcaaaacgc tcagtctttc atttggcttc atccagtcac ttagcatgct gatcaccttt
     4801 accgttattc tttggcaaag cgcgggtacg ctctcattca ctgttggtgg aacagaatgg
     4861 aatatccagg ggtatatggt ctataccgtt atgctcattg tgatcggtgg aactttattt
     4921 acccataagg ttggtaagcg tattcgtccg cttaatgtgg aaaaacaacg tagcgaagcg
     4981 acatttcgaa ctaatcttgt gcagcataat aagcaggcag aactgattgc gctaagcaat
     5041 gctgagtctc tacaacgcca ggagttgagc gacaattttc ataccattaa agagaactgg
     5101 aatcgtttaa tgaatcgtca gcggtggctt gattactggc aaaatattta ttcgcgctcg
     5161 cttagcgttc ttccctactt tctgttattg ccgcagttta ttagcgggca gatcaatctg
     5221 ggcggactaa tgaaatcgcg ccaggcattt atgctagtat cgaacaattt aagctggttt
     5281 atttataaat atgacgaact tgctgagctg gctgcggtta tcgatcgctt gtatgagttc
     5341 catcaactca ctgaacagcg ccctacgaat aagcctaaaa attgccaaca tgcggtacaa
     5401 gtggctaatg cgagtattcg tacgcctgat aataagatca tattagagaa cttgaacttt
     5461 catgtttcgc catgcaaatg gctattactg aaaggctact ctggtgcggg aaaaaccaca
     5521 ctgcttaaaa cattatccca ctgctggccg tggtttaaag gtgatatttc ttctcctgct
     5581 gacagttggt atgtgtcaca aacaccgtta atcaaaagcg gcttactgaa agagattatt
     5641 tgtaaagcac ttcccctgcc cgtagacgat aaatcgttga gcgaagtact gcatcaggtt
     5701 ggtctgggga aattggctgc gcgtattcat gaccacgatc gctggggaga tattctttcc
     5761 agcggcgaaa aacaacgtat cgccctggca cgattaattt tacgacgtcc gaaatggata
     5821 tttcttgacg aaactacctc tcatcttgag gaacaagagg ctatacgctt actgcgttta
     5881 gtgcgtgaaa aactacccac aagcggcgtc attatggtta cacatcaacc cggtgtctgg
     5941 aacctggctg atgatatttg tgacattagc acggttttat aacaacaata aaagccctgc
     6001 tgtacggaga tataaataaa tgaagcgagt tcttattcct ggcgtcattt tatgtggcgc
     6061 tgatgtggcg caggccgtcg atgacaaaaa catgtacatg catttttttg aagagatgac
     6121 ggtctatgct cctgtccctg tacccgtaaa cggcaacacg cattacacca gtgaaagcat
     6181 cgagcgttta ccgacctgga atggcaatat cagcgatctg ctgagaacca accctgcggt
     6241 acgcatggat tcaacgcaaa gtacctcgtt gaaccaggga gatattcgcc ctgagaaaat
     6301 ctctattcac ggtgcgtcgc cctaccagaa tgcctatttg attgacggta ttagtgcaac
     6361 taataacctg aacccagcga atgagtccga tgccagtagt gcaaccaata ttagcgggat
     6421 gtcacagggg tattatcttg atgtcagctt actggacaat gtgacgcttt atgacagttt
     6481 tgtgccgatt gaatttggtc gcttcaatgg cggggtaatt gatgcaaaga tcaaacgctt
     6541 caacgctgat gatagcaagg tgaaattggg ttatcgcact acgcgttcgg actggttaac
     6601 atcgcatatc gatgagaata acaagagcgc atttaatcaa ggctcttcag gaagtactta
     6661 ttactcccca gattttaaaa agaactttta taccttgtcg tttaatcagg aactcgctga
     6721 taacttcggc gttaccgccg gtttatcgcg ccgccagtct gatatcaccc gcgcggatta
     6781 tgtttcgaat gacggcattg tcgccggtcg ggcacagtat aaaaacgtta tcgatactgc
     6841 attgagcaaa tttacctggt ttgccagcga ccgctttacc cacgatttaa ccttaaaata
     6901 taccggctcc agccgtgatt ataataccag caccttcccg cagtctgatc gcgaaatggg
     6961 taataaatcc tatggtctgg catgggatat ggatacgcag ctcgcatggg ccaaactgcg
     7021 taccaccgtt ggttgggatc atattagtga ttatacccgt cacgatcatg acatctggta
     7081 caccgaactt tcatgtacat atggtgatat tacagggcgt tgcacccgtg gcggattagg
     7141 acacatttcc caggctgtag ataattacac cttcaaaaca cggctggact ggcaaaaatt
     7201 cgccgtgggt aatgtttcgc atcaacccta cttcggcgcg gaatacatct attccgatgc
     7261 gtggactgaa cgccataacc agtctgaatc ctatgtgatt aatgctgccg gaaagaaaac
     7321 taaccatacc atttaccata aaggtaaagg ccgcctggga attgacaact acacgctgta
     7381 tatggcggat cgcattagct ggcgtaatgt gtcattaatg cccggcgtgc ggtatgacta
     7441 tgacaactat ctgtcaaacc acaatatctc cccgcgcttt atgacggaat gggatatttt
     7501 tgctaatcaa acctcaatga ttacagcagg ttataaccgt tactatggcg ggaatattct
     7561 tgatatggga ttacgtgata tccgcaatag ctggacggaa tcggtatcag gtaataaaac
     7621 tctgacgcgt tatcaggatt tgaaaacgcc ttataacgat gaactggcaa tgggattgca
     7681 gcagaaaatc ggtaagaacg ttattgcgcg tgcaaactat gtttaccgtg aagcgcatga
     7741 tcaaatcagc aaaagcagtc gtaccgacag cgcgactaaa accaccatta ctgaatataa
     7801 caacgatggc aaaaccaaaa cgcattcgtt cagcctcagt tttgaactgg ccgaacccct
     7861 gcatatccgc caggtagata ttaacccaca aattgtcttt agctatatca agagcaaggg
     7921 caacttgtcg ttaaacaatg gttatgagga gagcaatacc ggtgataacc aggtggttta
     7981 taacggtaat ctggtctctt acgatagcgt tccagtggca gattttaata acccattaaa
     8041 gatctcctta aacatggatt tcacgcatca accgagcggg ttagtgtggg cgaatacgct
     8101 ggcctggcaa gaagcgcgta aagctcgcat tatccttggt aagacaaatg cgcaatacat
     8161 cagcgaatat tcagattaca agcagtatgt tgacgaaaaa ctggatagca gcctgacctg
     8221 ggacacccgc ttgtcctgga cgccacaatt tctgaaacaa caaaacctga cgatcagtgc
     8281 cgatattctc aatgtactgg atagcaaaac cgctgttgat acaacgaaca ccggtgtggc
     8341 gacctacgcc agtggccgta ctttctggct tgatgtcagc atgaaatttt aatagttcgg
     8401 attttactta accgggtaaa ttcgcccggt tttcgcatgg agataattat gagaaacctc
     8461 tgtttcttac tgacgttagt ggcaactctg ttgctccccg ggcggctgat tgccgccgcc
     8521 ttaccgcagg atgaaaagtt aattaccggg caactggaca atggcttgcg atatatgatt
     8581 tatccgcatg ctcaaccaaa ggatcaggta aatttatggc tgcaaattca taccggttca
     8641 ttgcaggaag aagacaatga gcgcggcgtg gctcattttg tagaacatat gatgtttaac
     8701 ggcacaaaaa catggccggg taataaagtc atcgaaacat ttgagtcaat gggcctgcgc
     8761 tttggtcgcg atgttaatgc ctataccagc tatgacgaaa cggtgtatca ggtgagtttg
     8821 ccgactacgc agaaacaaaa tctgcaacaa gtgatggcaa tcttcagtga atggagtaat
     8881 gccgcaacct ttgaaaaact cgaagtagac gctgaacgtg gcgtaattac tgaggaatgg
     8941 cgtgcccatc aggatgcgaa atggcgcacc tctcaggcgc gccgcccttt cctgctggct
     9001 aatacccgta atttagaccg tgaacctatc ggcctgatgg atactgtcgc cacggtcaca
     9061 ccggcacaat tgcgccaatt ttatcaacgc tggtatcaac caaataatat gacctttatc
     9121 gtggtcggcg atatcgacag taaagaagcg ctggcgctga taaaggataa tttaagtaag
     9181 cttccggcta acaaagcagc tgaaaatcgc gtctggccga caaaagccga aaaccacctg
     9241 cgctttaata tcattaatga taaagaaaac cgggtgaacg gcatcgcact ctattatcgc
     9301 ctgccaatgg tacaagtgaa cgatgagcaa agctttatcg aacaagctga atggagcatg
     9361 ttagttcagc tgttcaatca acgtctgcag gaacgcatac agtcgggcga gttgaagact
     9421 atttctggcg gcactgcgcg cagcgttaaa attgcacccg attatcagtc gctgtttttc
     9481 cgtgtaaatg cacgagacga taatatgcag gatgctgcga atgcattaat ggcagagttg
     9541 gcaaccattg atcagcatgg tttttctgct gaagaactcg atgatgtcaa atctacccgc
     9601 ctcacctggc tgaaaaatgc ggttgatcag caagctgaac gtgatttacg tatgctgacc
     9661 agtcgcctgg catccagctc attaaataat actccgttct tgtcgccgga agagacatat
     9721 caactttcga aacgtctgtg gcagcaaatt accgtgcaaa gtctggcgga aaaatggcag
     9781 cagttaagaa agaaccagga cgcattttgg gagcaaatgg taaacaatga gcttgccgcc
     9841 aaaaaagcat tgtctcctgc ggctatcctg gcgctggaaa aagagtacgc caacaaaaag
     9901 ctggcggctt acatcttccc aggcagaaat ttatcgttaa cagtagacgc tgacccacag
     9961 gcggaaatta gcagcaaaga aacgctggct gagaatctga catcattaac actttccaat
    10021 ggtgccaggg ttattctggc aaaatccgcg ggtgaagagc aaaagctaca aattactgcc
    10081 gtatcgaata aaggcgattt aagtttccct gcgcagcaaa aatcacttat cgcgctggca
    10141 aataaagcag taagcggaag cggcgttggt gagctctcct cttccagcct gaaacgctgg
    10201 agtgcggaaa attcggtaac catgagcagt aaagtcagtg gcatgaatac gttgctctcc
    10261 gttagcgcgc ggactaataa ccctgaacct ggttttcagt tgattaacca gcgaatcacc
    10321 cacagcacga ttaacgacaa tatttgggca tcgctacaaa atgctcaaat tcaggcgttg
    10381 aaaacgctcg accagcgtcc agcggagaaa ttcgcccagc agatgtatga gacgcgctat
    10441 gctgatgacc gcacgaaatt actgcaagaa aatcagattg tacagtttac tgccgcagat
    10501 gcgctggctg ccgatcgcca gttgttttca tctccagcgg atatcacgtt tgtcattgtc
    10561 ggtaatgtct cagaagacaa actcgtggcg ttaattacgc gttacttagg atcaatcaaa
    10621 cactctgatt cgccattagc cgcaggtaaa ccattaactc gcgcgacgga caacgcatcg
    10681 gttactgtaa aagaacaaaa tgaacctgtg gcacaggttt cacagtggaa gcgttatgat
    10741 tcccggacac ctgttaatct ggcgacgcgt atggcgctcg atgcttttaa cgtcgcactg
    10801 gcaaaagatc tacgtgttaa tattcgtgaa caggcatctg gagcatacag cgtttcttct
    10861 cgcctctcgg ttgatcctca ggccaaagat atcagtcatt tgctggcttt tacttgtcaa
    10921 ccagaacgac atgatgaact gttaacgtta gcgaatgaag tgatggttaa gcgtctggct
    10981 aaagggatca gtgagcaaga actgaatgaa taccagcaaa acgttcagcg cagcctcgat
    11041 atccaacagc gtagcgttca acaattagcg aacactattg taaatagtct tattcaatat
    11101 gacgatcctg cagcatggac tgagcaggag caattgttga aacaaatgac ggtagagaat
    11161 gttaacactg ccgttaaaca atatctttct catccggtaa atacttatac cggagtatta
    11221 ttgccaaaat aataacagcc cc
//
LOCUS       DAZKHC020000119        11241 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZKHC020000119 DAZKHC020000000
VERSION     DAZKHC020000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.119"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..479)
                     /locus_tag="ACHOSM_RS19870"
                     /old_locus_tag="pgaptmp_003974"
     CDS             complement(<1..479)
                     /locus_tag="ACHOSM_RS19870"
                     /old_locus_tag="pgaptmp_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4560376.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVLAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     "
     gene            835..1410
                     /gene="ssuE"
                     /locus_tag="ACHOSM_RS19875"
                     /old_locus_tag="pgaptmp_003975"
     CDS             835..1410
                     /gene="ssuE"
                     /locus_tag="ACHOSM_RS19875"
                     /old_locus_tag="pgaptmp_003975"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGU4560377.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKNLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            1403..2362
                     /gene="ssuA"
                     /locus_tag="ACHOSM_RS19880"
                     /old_locus_tag="pgaptmp_003976"
     CDS             1403..2362
                     /gene="ssuA"
                     /locus_tag="ACHOSM_RS19880"
                     /old_locus_tag="pgaptmp_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HGU4560378.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWTIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARSY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            2359..3504
                     /gene="ssuD"
                     /locus_tag="ACHOSM_RS19885"
                     /old_locus_tag="pgaptmp_003977"
     CDS             2359..3504
                     /gene="ssuD"
                     /locus_tag="ACHOSM_RS19885"
                     /old_locus_tag="pgaptmp_003977"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HGU4560379.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLQGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            3516..4307
                     /gene="ssuC"
                     /locus_tag="ACHOSM_RS19890"
                     /old_locus_tag="pgaptmp_003978"
     CDS             3516..4307
                     /gene="ssuC"
                     /locus_tag="ACHOSM_RS19890"
                     /old_locus_tag="pgaptmp_003978"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HGU4560380.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
     gene            4304..5071
                     /gene="ssuB"
                     /locus_tag="ACHOSM_RS19895"
                     /old_locus_tag="pgaptmp_003979"
     CDS             4304..5071
                     /gene="ssuB"
                     /locus_tag="ACHOSM_RS19895"
                     /old_locus_tag="pgaptmp_003979"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU4560381.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(5278..7890)
                     /gene="pepN"
                     /locus_tag="ACHOSM_RS19900"
                     /old_locus_tag="pgaptmp_003980"
     CDS             complement(5278..7890)
                     /gene="pepN"
                     /locus_tag="ACHOSM_RS19900"
                     /old_locus_tag="pgaptmp_003980"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HGU4560382.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            8156..9358
                     /gene="pncB"
                     /locus_tag="ACHOSM_RS19905"
                     /old_locus_tag="pgaptmp_003981"
     CDS             8156..9358
                     /gene="pncB"
                     /locus_tag="ACHOSM_RS19905"
                     /old_locus_tag="pgaptmp_003981"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGU4560383.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9527..10927
                     /gene="asnS"
                     /locus_tag="ACHOSM_RS19910"
                     /old_locus_tag="pgaptmp_003982"
     CDS             9527..10927
                     /gene="asnS"
                     /locus_tag="ACHOSM_RS19910"
                     /old_locus_tag="pgaptmp_003982"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGU4560384.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMRFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2596 a         2843 c         2964 g         2838 t
ORIGIN      
        1 gcgatataat caactttaaa ggtcactgtt tgctcagcag aatccgctaa ctgaatattc
       61 gacgtttcgg taccgggagt taccgatgta gaaccatctg gcagatacat ttgtagagca
      121 acattcgtcg ctgcatcggt ggtagcctgg ttggcaaatg ctgtaggttg cacgccgtca
      181 gccgtaccac taaaggtaaa cgtcgcattt ttagttacag taacatcaca gccttctaag
      241 gcaattggta catcagtttt aacgccagag actgctccat tagtttgcaa cttggtggcg
      301 ctgacatttg gcaatgtgac tactgaatct ttcgtcgcag ctaccagggt acaagctgga
      361 gcaagaactt taccattaaa tgttactttg ccatccgtga tagcattatc atcagctgcc
      421 ataacgctgg acgttgcaca taccacagtt ataaacgccg tcaatacact ttttttcatc
      481 gtcatcatcc tgatctcttc gtaaatatga taatgccttt aaatgacatt agacttaata
      541 ataacaataa ttgaccgcca tttaaatcaa gcatactaaa tccaataagt gacaaatctt
      601 taatttatag atttcatgaa tatttaaata ttaactgaac gagtaattca ttgattcaac
      661 atctaatgac tatccaacaa ataaatatgc gtgatatata aagccctttc tttagtttat
      721 tttcagaaaa agatacacaa aatttatatt tggcattttc ttgtctctcc gacagactga
      781 ccgaattgtt atcaatgtta acaaaaaaag aacaattggt tataaggaga gagtatgcgt
      841 gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct ccagctcctt gctggaatat
      901 gcgcgggaaa aactaaatgg cctggatgta gaggtttatc actggaatct gcaaaacttc
      961 gccccggaag atctgcttta cgctcgtttc gatagtccgg cactcaagac cttcaccgaa
     1021 cagctgcaac aggctgatgg actgatagtc gctacacctg tgtataaagc cgcctattcc
     1081 ggcgcgctga aaaacctgct cgacctgctg ccagagcgcg ctttgcaagg caaagtggtg
     1141 ctaccgctgg cgacgggcgg tacggtggct catctgctgg cggtcgatta tgcccttaag
     1201 ccagttttaa gtgcgatgaa agcccaggag atcctgcacg gcgtgtttgc cgatgactca
     1261 caagttattg attaccatca caaaccccag ttcacgccaa atctgcaaac ccgtcttgat
     1321 accgcgctag aaactttctg gcaggcattg caccgccgcg atgttcaggt tcctgacctt
     1381 ctgtctctgc gaggtaatgt ccatgcgtaa catcattaaa ctggcgctgg cgggattgct
     1441 tagcgtctct acttttgcgg ttgctgcaga atcctcgcct gaagcgttac gtataggcta
     1501 tcagaaaggc agtattggta tggtattggc aaaaagccac cagttactgg aaaaacgcta
     1561 tccgcaatca aaaatctcct gggtggagtt ccccgcgggt ccgcaaatgt tggaagcgtt
     1621 aaacgttggc agtattgatc tcggcagtac cggggatatt ccgccaatct ttgcccaggc
     1681 tgccggggct gatttggtat atgtgggcgt cgagccaccg aagcccaaag ccgaagtgat
     1741 tctggtggca gaaaacagcc cgatcaaaac cgtagccgat cttaaaggtc acaaagttgc
     1801 ctttcagaaa ggttccagtt cacacaacct tttactgcgt gcactgcgtc aggccggact
     1861 taagtttact gacatccagc ccacttatct gacgccagct gatgcccgcg ccgcgttcca
     1921 gcaaggtaac gttgacgctt ggactatctg ggatccctac tactccgctg cgttattaca
     1981 gggcggcgtg cgggtgctga aagacggcac cgatctcaat caaactggat cgttttatct
     2041 ggcagctcgc tcctatgcag aaaaaaacgg cgcttttatt cagggcgtac tggcaacctt
     2101 tagtgaggcc gatgcgttaa cccgcagcca gcgcgagcaa agcatcgctt tactggcaaa
     2161 aacgatgggc ttaccggcac cggtgattgc ctcgtatctg gatcatcgtc ctcccaccac
     2221 catcaaaccg gtgagcgctg aagttgccgc cttacagcag caaacggcag atctgtttta
     2281 tgaaaaccgt ctggtgccga aaaaagttga tattcgccag cgcatctggc agcccactca
     2341 actggaagga aaacaattat gagtctgaat atgttctggt ttttaccgac ccatggtgac
     2401 gggcattatc ttggaacgga agaaggttca cgcccggttg atcacggcta tctgcaacaa
     2461 attgcgcaag cggcggatcg tctgggctat accggtgtgc tgatcccaac ggggcgatcg
     2521 tgtgaagatg cgtggctggt ggcggcgtcg atgatcccgg tgacgcagcg gctgaagttt
     2581 cttgtcgccc tgcgtcccag cgtaacctca cctaccgttg ccgcccgcca ggccgccacg
     2641 cttgaccgtc tctcgaatgg tcgcgcgttg tttaatctgg tcacaggcag cgatccacaa
     2701 gagctggcag gcgacggcgt gttccttgat catagcgagc gctacgaagc ctcggcggaa
     2761 tttacccagg tctggcgacg tttattgcag ggagaaaccg tcgatttcaa cggtaaacat
     2821 attcatgtgc ggggagccaa actgctcttc ccgccgattc aacagcctta tcctccgctt
     2881 tactttggcg gatcgtcaga tgtcgcccag gagctggcgg cagaacaagt tgatctctac
     2941 ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa tcgaacaagt gcgggcgaaa
     3001 gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc tgcatgtgat tgttcgtgaa
     3061 actaacgacg aagcgtggca ggccgccgag cggttaatct cgcatcttga tgatgaaact
     3121 atcgccaaag cacaggccgc attcgcccgg acggattccg taggacaaca gcgaatggcg
     3181 gcgctacata acggcaagcg cgacaatctg gagatcagcc ccaatttatg ggcgggcgtt
     3241 ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg atggtcctac ggtcgccgcg
     3301 cgaatcaacg aatacgccgc gcttggcatc gacagctttg tgctttcggg ctacccgcat
     3361 ctggaagaag cgtatcgggt tggcgagttg ctgttcccac atctggatgt cgccattccg
     3421 gaaattcccc agccgcagcc gctgaatccg caaggcgaag cggtggcgaa tgattttatc
     3481 ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc aacgccagtg aagaaatggt
     3541 tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt ggcggtgtgg caactggcct
     3601 cctcggttgg ctggctgtcg acgcgtattt tgccttcacc ggaaggagtg gtgacggcgt
     3661 tctggacgct ctccgccagc ggcgaactgt ggcagcatct ggcgatcagt tcctggcggg
     3721 cgctgattgg cttttcaatt ggcggatcgc tgggcctgat tctgggactg attagcggac
     3781 tgtcgcgctg gggagaaagg ctgctggata cctcaattca gatgttgcgc aacgtgccgc
     3841 atctggcgct gatcccgctg gtgattttgt ggtttggcat tgatgaatcc gcgaaaatct
     3901 ttctggtggc gctcggtacg ctgttcccca tttatatcaa cacctggcat ggcatccgta
     3961 atatcgatcg cgggctggtg gagatggcgc gtagctatgg attatccggc ataccgctgt
     4021 ttatccatgt gatcctgccc gatgccctgc cctcaattat ggtcggcgtg cgttttgcgt
     4081 tgggcctgat gtggctgacg ctgattgttg ccgaaaccat ttctgccaat tccggcattg
     4141 gttatctggc gatgaatgcg cgagagtttt tgcaaacaga cgtggtggta gtcgctatta
     4201 ttctttacgc cctgctcggc aaacttgctg acgtcagcgc gcagctgtta gagcgcctct
     4261 ggttgcgctg gaacccggct tatcatttga aggaggccac gatatgaata ctgctcgtct
     4321 gaaccagggc acaccattgt tgctcaatgc agtaagcaaa cattacgcgg aaaatatcgt
     4381 cctgaaccaa ctggatttac atattccggc aggtcagttt gtggcggtgg tgggccgcag
     4441 cggtggtggt aaaagtaccc tgctgcgcct gctggccggt ctggaaacgc caaccgcagg
     4501 cgatgtgtta gcgggcacca caccgctggc tgaaattcag gatgatacgc gaatgatgtt
     4561 tcaggatgcg cgtctgctgc cgtggaaatc ggtgattgat aacgttgggt taggccttaa
     4621 aggtcagtgg cgcgatgccg cgcgtcgggc gttggctgcg gtaggactgg agaatcgcgc
     4681 cggggaatgg cctgccgcac tttctggcgg gcagaaacag cgtgtggcgc tggcaagagc
     4741 gttgattcat cgaccgggtt tattgttgct tgatgaaccg ctcggggcac tggacgcctt
     4801 aacgcgactc gagatgcagg atttgattgt gtcactttgg cagcagcacg gctttaccgt
     4861 actgttggtg acgcatgatg tgagcgaagc ggtagcaatg gctgaccggg tgttgttaat
     4921 tgaagaggga aaaattggtc tggatttgac agtggatatc ccccgtccgc gtcgattagg
     4981 gtcggtgcga ttagcggagc tggaagcgga agtattgcaa cgggtaatgc ggcgtgggca
     5041 ttctgaacaa ctgattcggc gtcatggtta atatgccgga tgtggcgcac aaaaccagca
     5101 ttttcaacgg acttgtaggc ctgataagac acgtcaagca tcgcatcagg cagttgggca
     5161 cggctgccgg atgcggcgtg aacgccttat ccagcctaca aaaccagcaa tttcaacgga
     5221 gttgtaggcc tgataagacg cgtcaagcgt cgcatcaggc attttggctc aaaagtatca
     5281 agccagtgct ttagttatct tctcgtaaag atcgccagag agattttcca gccctttcaa
     5341 ctgttccagc gccgcgcgca ttttctcctg acgtttggca tcgtaacgtt tcaggcgaat
     5401 cagcggttca atcagacgtg aagccacctg cgggttacgg ctgttgaggt cggtaagcat
     5461 ttccaccagg aactggtaac cgctgccatc ttcggcatgg aacgctgccg ggttgctgcc
     5521 cgcaaacgca ccaatcaacg aacgaatacg gttcgggttg ctcatggtaa atgagcgatg
     5581 ctgcaacaga ccacgcaccg tctccagcac attcgccgcc gggctggtgg cttgcaggat
     5641 aaaccattta tccatcacca gaccgtcctg atgccactta tcgtcgtact cctgcatcag
     5701 cgcgtcacgg caaggcagct gtgcagcaac cgccgcagaa agcgctgcca gcgcatcggt
     5761 catattgttt gcttcatggt attgcttgct caccagcacg tcagccagat gcgtttcacc
     5821 aaaagcaagg aagcgcaggc aggcattacg cagagtgcgt tttgcaatat cttcatgctc
     5881 aacacggtac tcgctctggt aattcgcgtt gtaaatagcc agcagctcat ccgccagttc
     5941 agtcgccaga gtacgagtga gtgcttcgcg tacttcggca atagcaatcg ggtcgatgat
     6001 atcgaacagt tcagccattt cattgacaga aggcagcgtc aggatttctg ccgccagcgc
     6061 cggatcaatc ttctcatcga gcagcaccgc gcggaaagcg tcagccacat gtaccggcag
     6121 agacagcggc tgcccttgct gatgacgggc gacgttcagc ttgatgtagg ttgccagcaa
     6181 actttgcgcc gcatcccaac gggagaaatc attacgcgca tgacgcatca ggaaggtcag
     6241 ttgctgatcg ctccacttat attccagttt cactggcgca gagaattcgc acagcagcgc
     6301 aggcaccggc tggaagtaga cattatcaaa aacaaaggtc tgttccgcct gggtgacgtt
     6361 cagcacggaa ttcaccggat gaccgccttt ctgcaacggg atcactttgc cttcgttgtc
     6421 atacaattcg atggcaaatg gaatatgcag cggctgtttt tctgcctgat ccggcgtggc
     6481 tggcgtgcgc tggctgatgg tcagggtgta ctgctcggtt tccggattgt agtcgtcttt
     6541 gacggtcaca atcggcgtgc cggactggct gtaccaacgg cggaaatggg agagatcgac
     6601 attcgacgca tcttccatcg cctgcacaaa gtcgtcgcag gttgccgcgc tgccatcatg
     6661 gcgctcgaaa tagagttgca tccctttctg gaagttttct tcgcccagca gggtgtggat
     6721 catacggatc acttccgcgc ccttctcgta aacggtcagg gtgtagaagt tgttcatctc
     6781 aatgaccatg tccgggcgga tcgggtgcgc catcgggctg gcgtcttccg caaactgcaa
     6841 tccgcgcatg gtgcgcacat tattgatgcg gttaactgca cgggaaccaa ggtcagagct
     6901 gaattcctga tcgcggaaga cggttaaacc ttctttcagg ctgagctgga accagtcgcg
     6961 gcaggtgact cggttaccgg tccagttatg gaaatattca tggccgataa cgcgttcaat
     7021 atcgaggtaa tctttgtcag tggcggtgtc ggtgcgggcc agcacatatt tggagttaaa
     7081 gatattcaga cccttattct ccattgcgcc catattgaag aaatccaccg cgacgatcat
     7141 atagatgtcg aggtcatact ccaggccgaa gcgttcttca tcccatttca ttgagttttt
     7201 cagcgaggtc atcgcccacg gcgcgcgatc aaggttgccg cgatcgacgt acagctccag
     7261 cgccacttcg cgaccagaac gcgtggtaaa ggtgtcgcgc agtacatcaa agtcgcctgc
     7321 caccagcgca aacaggtagc acggtttcgg gaacgggtcc tgccactgta cccaatggcg
     7381 tccgttttcc agttcacctt gcgcaacgcg gttaccgttg gaaagcagga agggatattt
     7441 ggttttatcg gcaataattt tggtggtaaa acgcgccagc acgtccgggc ggtcgagata
     7501 atacgtaata tggcggaaac cttcggcttc acactgggtg caaagcgcat cgcctgactg
     7561 ataaagccct tccagcgcgg tattcgccgc cgggcttatt tcattaacga tcttaagcgt
     7621 aaaatgctcc ggcaaattac tgatgaccag tgcgccctct tcttctttcc aggcggtcca
     7681 cggctcatca ttaatatgaa cagaaaccag tttgaggtct tcgccgttga gacgaagtgg
     7741 agcatctgat gcaccatgac ggacagcctg gctgaccgcg gtaacgaccg tcttttgcgc
     7801 gtcgaggtca aaggtcaagt caatatcagt aatctggtaa tccggcgcac gataatcgtg
     7861 acggtatttg gcttgtggct gttgagtcat aaataacctt tagcatcttt tatagagtct
     7921 ggtgttcagt ctattcctgt tgcgtaaatc gcgctatgca gaatcttcat cttttcaggt
     7981 acaaacgcct ttattgctac atttttataa catacaccgc gtaatgccat cgaccagaaa
     8041 ggtggcatat ggtgtgatcg gggttcaata aattgcgaaa caaggtatac tccagcagtt
     8101 cctgaagatg tttattgtac taaacgctcc tgtacgagga cgctactgcg cacctatgac
     8161 acaattcgct tctcctgttc tgcactcgtt gctggataca gatgcttata agttgcatat
     8221 gcagcaagcc gtgtttcatc actattacga tgtgcatgtc gcggcggagt ttcgttgccg
     8281 aggtgacgat ctgctgggta tttatgccga tgctattcgt gaacaggttc aggcgatgca
     8341 gcacctgcgc ctgcaggatg atgaatatca gtggctctct gccctgcctt tctttaaggc
     8401 cgactatctt aactggttac gcgagttccg ctttaacccg gaacaagtca ccgtgtccaa
     8461 cgataatggc aagctggata ttcgcttaag cggtccgtgg cgtgaagtca tcctctggga
     8521 agttcctttg ctggcggtta tcagtgaaat ggtacatcgc tatcgctcac cgcaggccga
     8581 cgttgcgcaa gccctcgaca cgctggaaag caaattagtc gacttctcgg cgttaaccgc
     8641 cggtcttgat atgtcgcgct tccatctgat ggattttggc acccgtcgcc gtttttctcg
     8701 cgaagtacaa gaaaccatcg ttaagcgtct gcaacaggaa tcctggttcg tgggcaccag
     8761 caactacgat ctggcgcgtc ggctttccct cacgccgatg ggaacacagg cacacgaatg
     8821 gttccaggca catcagcaaa tcagcccgga tctagccaac agccagcgag ctgcacttgc
     8881 tgcctggctg gaagagtatc ccgaccaact tggcattgca ttaaccgact gcatcactat
     8941 ggatgctttc ctgcgtgatt tcggtgtcga gttcgctagt cggtatcagg gcctgcgtca
     9001 tgactctggc gacccggttg aatggggtga aaaagccatt gcacattatg aaaagctggg
     9061 aattgatcca cagagtaaaa cgctggtttt ctctgacaat ctggatttac gcaaagcggt
     9121 tgagctatac cgccacttct cttcccgcgt gcaattaagt tttggtattg ggactcgcct
     9181 gacctgcgat atcccccagg taaaacccct gaatattgtg attaagttgg tagagtgtaa
     9241 cggtaaaccg gtggcgaaac tttctgacag ccctggcaaa actatctgcc acgataaagc
     9301 gtttgttcgg gcgctgcgca aagcgttcga ccttccgcat attaaaaaag ccagttaata
     9361 tcatcaggga gctaatcggc tccctttttt tacctttaat tccgaaatct ttcgctgcat
     9421 ttgcgaattc tgcttgtctg attgcagatg ccaggtaaca taggtatccc cccatttacg
     9481 ggtggacaag tgtttatttt ttccgactat taacagagag aatattatga gcgttgtgcc
     9541 tgtagccgac gtactccagg gccgcgtagc cgttgacagc gaagtcaccg tacgcggatg
     9601 ggtacgtacc cgccgagatt caaaagctgg catctccttc ctcgccgttt atgacggttc
     9661 ctgctttgat cctgtacagg ctgtcatcaa taattctctg cccaattaca atgaagacgt
     9721 cctgcgtctg accaccggct gctcggtcat tgtgacgggt aaagtcgtgg cgtcgccggg
     9781 ccaggggcaa caatttgaaa ttcaggccag caaggttgaa gttgctggtt gggttgaaga
     9841 tccagacact tacccgatgg cggcaaaacg ccacagcatt gagtatctgc gtgaagtcgc
     9901 tcacctgcgt ccgcgcacaa acctgattgg tgccgtcgcg cgcgttcgtc atacgctggc
     9961 gcaggcgctg catcgcttct ttaacgagca gggattcttc tgggtttcaa cgccactgat
    10021 tactgcgtct gataccgaag gtgctggcga aatgttccgc gtttctacgc tggatctgga
    10081 aaacctgccg cgtaacgatc agggcaaagt ggatttcgac aaagacttct ttggtaaaga
    10141 gtctttcctg accgtatctg gccagttgaa cggcgaaacc tacgcttgcg cattatccaa
    10201 aatttatacc ttcggcccga ctttccgtgc tgaaaactcc aacaccagcc gtcacctggc
    10261 ggaattctgg atgctggagc cggaagtggc gtttgctaac ctgaacgata tcgcgggtct
    10321 ggctgaagcc atgctgaaat atgtcttcaa agcggttctc gaagaacgcg ctgacgacat
    10381 gagattcttc gctgaacgcg tagataaaga tgccgtttca cgtctggaac gcttcattga
    10441 agccgatttt gcgcaggtgg attacaccga cgcagtgacc attctcgaaa actgcggcag
    10501 gaagtttgaa aacccggttt actggggcgt tgatctctct tctgagcatg agcgttatct
    10561 ggcggaagaa cactttaaag caccggtagt ggttaaaaac tatccgaaag atattaaagc
    10621 gttctatatg cgccttaacg aagacggtaa aaccgttgcg gctatggacg ttctggctcc
    10681 gggcatcggt gagatcattg gtggttccca gcgtgaagaa cgtctggacg tgctggacga
    10741 gcgtatgctg gaaatgggcc tgaataaaga agattactgg tggtatcgcg atctgcgtcg
    10801 ctacggtact gttccgcatt caggtttcgg tcttggtttt gaacgcctga ttgcttacgt
    10861 aactggtgtg caaaacgtgc gtgatgtgat tccgttccca cgtactccgc gcaacgccag
    10921 cttctgattt tcttcctcaa agagccagcg cctgctggct cttttttatc ctttctgtca
    10981 agttatctgt ttgttaagtc aagcaatcta tttgcaatcc cgccataacg atcatcctgt
    11041 tacggaatat tacattgcaa catttacgca caaaaactaa cccgcattct tattgcggat
    11101 tagttttttc ttagctgata gcacaatttt catactattt tttggcattc tggatgtctg
    11161 aaagaggatt ttgtgccagg tcgataaagt ttccatcaga aaaaaatttc cgtttagtta
    11221 atttaaatat aagtaaatca t
//
LOCUS       DAZKHC020000120        11237 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZKHC020000120 DAZKHC020000000
VERSION     DAZKHC020000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.120"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..132)
                     /locus_tag="ACHOSM_RS19915"
                     /old_locus_tag="pgaptmp_003983"
                     /pseudo
     CDS             complement(1..132)
                     /locus_tag="ACHOSM_RS19915"
                     /old_locus_tag="pgaptmp_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(206..9715)
                     /gene="toxB"
                     /locus_tag="ACHOSM_RS19920"
                     /old_locus_tag="pgaptmp_003984"
     CDS             complement(206..9715)
                     /gene="toxB"
                     /locus_tag="ACHOSM_RS19920"
                     /old_locus_tag="pgaptmp_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HGU4560385.1"
                     /translation="MIHPGSSLDKAINNTRVKNVSTDVKHGQIQERKRNFIYKKNDDI
                     SSRFKLYSSLVKQKNATEDVVLIGKMILDEVRSYRTIHNDRNIVSNSGNWKTSFLCNL
                     ARLLYSIFNGSNYFCSREGENNSSPSSTLLTIHQPEKQELLQQKSIKHLPTSNNIDGY
                     IKIRKTRGAEDQTTTITQSLIINELLNGVDRNTIPFQKISELNDIIHSYENMQIKNSR
                     KGIEILVKQGELLSSLINDNKGNKQLSDNASKIINLLGIEYQSHKVDIEPFIHAVWVA
                     GAPPDNTFSYITAFLNTYKDYTYLLWIDPNAFGAAKFSGILKNIAMNYAIMRLRRTNP
                     HLAEEMNEVILKIQNIQNETIEFKETRERLKELENRYKSLTSETKEKFNVFFLESMIG
                     MQDNYFTYCISNGISNTDDISRLDFLTNVLKLSPEVQNDFKSTVEKNKRDIDLLKNTI
                     SQKFGDRFQLRDINTLESFKKPQDYFFYQQEMLLRWNYAAASDQVRINILKEYGGIYT
                     DTDILPAYSDKVSQIINEKSDDKRFFEDLKLRRIISESILSLIKGEKYSIKHDGLDET
                     TLNQLNNILSEIEKLTIDDYFKPVETKVVRDTFKIFKRYQKWTENTWNIRGNNNFMLT
                     HKGSKCIDFILSGQKKQYLELQRIRDNISYNNLFYTTEDLKSLNNVAIGGIPAKKYLE
                     HGLFSEYRQDGTIPYVVSTLNISGPDMIMRQMKKYYKSLGRIGEVHIKDNKLSDVNFL
                     GVYASSNKENKSFNWLNPVSVGINDITPDDESSWAVRNNDINKILFEKINCHVPEKLP
                     TSLYYEIDSRSFFQGWDNKSIKHVTEINKDLIKDINLLLTSSNIDVKLLIKLDRELYA
                     ISSKIDNPLALRSIRTLQLQLANYVTSNTFEPENTINFIYDFYRKKQDDLLSAIKLFS
                     RNDADTKIIVWYNSVMEKNVFLREVISCVLRSKKVDSYINENKKNLSKEDAGALRDYA
                     KLKMKELFSMLDDDGYKKIITTNAYIKERDKLSGIIYNIENSIISGHESFDIIRSNQH
                     EWGDLSTVEQFKKFEFYVKSELSSAKSIFDDIKNKYITDPETKRNVLYHQLDSDIKER
                     IAFLDISHYAYPGSLLEKLQLSGYVFSDINIIAEYLLASYGVSGHYSHGVVYPAPSDK
                     LLELLRRHTKSNSEWIEKITPYVYDILSDNVSNVLRPPLSEEQKKILNDIKLEISKSV
                     SEQYFMKLTEQKSSVIGIKYSVDFDRYNENLFLSLPINQNLTLPFMYRYFEMLYDIHI
                     GIIENKANREFIYSKFSSLNLDFLINDERVLNLEGLIKKYKYLSLSEIHRTLTNSTSF
                     ADISIPLLQTICPSITTIIKKTEYYGHQLTNAMTVASVVKPYDFSNLGAINSIDKSVS
                     DVPALHTIVEQAKYNLLSWNDFYNTHASIWDTIARQHKSTNIEFHPQSLLFDRDSKGK
                     CLGLSLLYLDTGGYGGGYQKLRHNIDTASTLYQTKYNDNLKLSNRDDFFLRKTQRIIT
                     MSNELGNNRLKNAQLEVLELKDPILTEGILYQRRISSLLITTEYHSLALQQISSFWRV
                     TDPNFGHCDFHSLAQALTFIKNITSNRNFSSLYGSGIVKIYFSESLNNWKYIKLPLVQ
                     TGSLLRDIYLTTPEKLSTSGDSLNIMGHLVPVSFIYDIGGVINGNRISESTDVKNKIR
                     SLKINGDILQHYINTHYLSEEQTQKIKDIVDFLGIQDNTIKVKLESDIKPISEIQQPL
                     HSILSRQKEHVKNLLSGLLDEFSNKLRKQGLSLKTNVLSVNNFKESKINSDTVEVTVT
                     DLQGRLYRVDIDTRVIGLTFKEGINSLSEALEHMNIDAIMSVIGLVQYARMIKMNDNI
                     SAIDHAGAVSDIKNIVDKFLGGILTLTNNRVYNPGGVSGASLEGFISSGLEVCASRMG
                     GTAGRYLSNVAKVIKLPLLDIGINIWSLYDSSLNHAKATTQIEYISTAIDVSFSSINT
                     ALSIGAIAYPPLAIAIVPITIFSHEVKNYAVYVNQINERHKLWLEAEKYLDNGSAKVL
                     SINKATGIIDLSNNQVLGNIYLDMRENPPILHGEKSYNSGKNIGSHPDMTDREIMESR
                     AYNFACTKLSDAGEPDIFGWGDKEICNSMELSESQLANGYSNRQWPSQIPVIPEGIYN
                     TVYLGYGETLRANTEVTLSSTGYFYEIARAYTDDELSEPLLTVCNQHSHVIGGKEPLT
                     IIIPAIEHGMLGSNLHMIERFKNYNFSISGGKGGIKLMVGGIGDYNIECTPGVRNIIS
                     FEQLSRDFNLDLDLSDGRKQNFNFHHPSGFYSGKVMSITQKGINAVVGTKLGYDKIRG
                     NNLDNTFSLGSGGGVIYSGGGSNTYFVPATLQDNLHIYISEKSNGNHIILGDMHSRLS
                     IECHFSNNEREFIKMGYYNGCDVILESDTIQKIKSFAKNITIQTADGVMANWDDKLNT
                     LSVYSIDMIAWRDKNKTAKEPLPVDVIQLTNWRMHNTCSLFYDKYQVDIEANKLTYTV
                     LFPDTELPVQLNYTSIIYGNHGAKYTFLNSGSKTIDIHILDKNSDYDTFDFRNIIFEH
                     YTNEIFISFDNQGGFVISILNNATSEAANINVFRKNMTSLDSSGSLIYLPSGDIYHIS
                     DIYKMSRGRKSFKLNVEKKPDIDDIINVAILETSYLQIKKIPNNDDSDYILCLDNPNL
                     SSYTLNFNDLSGYISSLWDNIRGSFTPFHKNTVNIAPNEKKYISLIGLDKLSFNIDVF
                     RQALEVKNKNSYKISKFTWETYGDIVVSPEDRISHLELDGFNYFSQPELDTPISDSFS
                     YLYDNFQIVDSDVHIKLLHLNRETKQITPHRIILKRYFIDSFAKTSITDREKNIYPVI
                     CDSPDHFTSDIYRHPFRIVLGNKALYPSEELVKFISTSKEYLSNMDVINNVIVPQKTT
                     KKNKLSIVSLNSNIKNDVVLSGVMTGTSKIFHLNNSGDLLLTTSKTHGGGVVVIFKDF
                     INNWWKYNLTLITVPIDNKLSDNRINITPMGIKIQETVSGNDRLFFYPTPLKNGSFIL
                     HNPLYSNSFPSLYSHELFDLIEAYRNPTYSYLQNNIINRYIHTVSEYTGKDGIANMSL
                     SIYAYSTCRFWSKESVAVGDTIKLSNYEHIEFSFNSFSKDYFREQNSDIYKIFFKIAS
                     SSNSVVVDKNTLTQKNFLENNTFFSITGIDESLYEKHIIFITLKVIVPSKK"
     gene            complement(9822..9974)
                     /locus_tag="ACHOSM_RS19925"
                     /old_locus_tag="pgaptmp_003985"
                     /pseudo
     CDS             complement(9822..9974)
                     /locus_tag="ACHOSM_RS19925"
                     /old_locus_tag="pgaptmp_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(9971..10091)
                     /locus_tag="ACHOSM_RS19930"
                     /old_locus_tag="pgaptmp_003986"
                     /pseudo
     CDS             complement(9971..10091)
                     /locus_tag="ACHOSM_RS19930"
                     /old_locus_tag="pgaptmp_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(10131..10394)
                     /locus_tag="ACHOSM_RS19935"
                     /old_locus_tag="pgaptmp_003987"
                     /pseudo
     CDS             complement(10131..10394)
                     /locus_tag="ACHOSM_RS19935"
                     /old_locus_tag="pgaptmp_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            10488..10837
                     /locus_tag="ACHOSM_RS19940"
                     /old_locus_tag="pgaptmp_003988"
                     /pseudo
     CDS             10488..10837
                     /locus_tag="ACHOSM_RS19940"
                     /old_locus_tag="pgaptmp_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3402 a         2031 c         1765 g         4039 t
ORIGIN      
        1 gtaacagacc ggactgaaat tccaggaagc ttttgccctc agtccgtgac ggcgcaggct
       61 ttccgtcacg gtttttactt taaaatggta accctgggca cgcagttcat ccatcaggcg
      121 tttcgcgaag tatgtcgtgg cctttggaac gtctgctgat gctgggtaag attagtcagc
      181 aaattccgca tacaggaaat cagcgctact tttttgaggg gacaataacc ttaagagtga
      241 taaagattat atgcttttca tagagagact catctatacc tgtaatactg aaaaatgtat
      301 tgttttctag gaagtttttt tgagtgagcg tatttttatc cacaacaacc gaattactcg
      361 aagaagcaat tttaaaaaat attttatata tatcactatt ttgttcacga aagtaatcct
      421 tactaaatga attaaagcta aactcaatat gctcataatt agacaatttt attgtatctc
      481 ctacagccac actttctttt gaccagaaac gacatgtcga gtatgcgtat atagataatg
      541 acatattggc aataccgtct tttccagtat actcagaaac agtatgtata tatctattga
      601 taatattgtt ctgtaaatac gaatacgttg gatttctata tgcttcaatc aaatcaaata
      661 attcatgtga atataaagaa gggaatgagt tagagtaaag tggattatgc aagataaaac
      721 ttccattttt taatggagta gggtaaaaaa acaacctgtc atttccactg actgtttcct
      781 gtatctttat ccccattggg gttatattta ttctattgtc tgacaattta ttatcaatag
      841 gaactgttat taaagtaaga ttatatttcc accagttatt tataaaatct ttaaaaataa
      901 ccacaacccc accaccatga gtttttgagg ttgttaacaa taaatcacct gaattattta
      961 gatgaaaaat ctttgatgtt ccggtcatta ctccggacaa tacaacatca tttttgatat
     1021 tgctatttaa actaactatg gataatttgt ttttctttgt tgttttttga ggaacaataa
     1081 cattgttaat cacatccata ttgcttaaat attcttttga ggtgcttatg aattttacaa
     1141 gttcttctga tggatacaat gccttattcc cgagaactat tctaaatgga tgcctatata
     1201 tgtcgcttgt gaagtgatca ggtgaatcac aaatcacagg atatatattc ttttctctgt
     1261 cagtgatact tgttttagca aatgagtcta taaagtatct tttaagaata atacgatgtg
     1321 gtgttatttg ctttgtttct ctgttgagat gaagaagctt aatatgcaca tcactatcaa
     1381 cgatctgaaa attatcatat aaatatgaaa atgagtcaga tattggagta tctaactcgg
     1441 gttgtgaaaa ataattaaac ccatctaact ccagatgaga aattctatct tcaggtgata
     1501 caacgatatc accatacgtt tcccatgtaa atttacttat cttgtaacta ttcttatttt
     1561 ttacctctaa tgcttgtcta aaaacatcaa tattgaatga caacttatca agtccgatta
     1621 gggaaatata ttttttttca ttaggggcga tgtttacagt atttttatga aaaggggtaa
     1681 aactacccct aatattatcc cataaacttg atatgtatcc agacaagtca ttaaagttca
     1741 atgtgtaaga tgagaggtta gggttgtcta aacataaaat ataatcacta tcatcattat
     1801 tggggatttt ttttatttgt agataagaag tttctaaaat agccacgttg atgatatcat
     1861 caatgtcagg tttcttttct acatttaatt taaacgattt tctacctctg gacattttat
     1921 agatatctga aatatgataa atatcaccgc taggcaaata tatcaatgaa ccactactat
     1981 ctaaagaagt catgtttttt cggaatacat taatattcgc cgcttcactc gtagcattat
     2041 ttaatattga tataacaaat cctccttgat tatcaaaact tataaatatc tcattggtgt
     2101 aatgttcaaa aataatatta cggaagtcaa aagtatcata atcactattt ttatcaagaa
     2161 tgtgaatatc tatagtttta cttcctgaat tgaggaaggt gtattttgcc ccatgattac
     2221 cataaataat actggtataa ttgagctgaa cgggtaactc cgtatcagga aataagactg
     2281 tatacgttaa cttattggct tcaatatcaa cttgatattt atcataaaat aaagagcatg
     2341 tgttatgcat cctccaatta gttaactgga ttacatcaac tggtaacggt tcttttgcag
     2401 ttttattttt atccctccat gcaatcatat ctattgaata aacagacaga gtatttagtt
     2461 tatcatccca atttgccata actccatccg ctgtctgtat tgttatattt tttgcaaatg
     2521 attttatttt ttgtatagtg tcactttcta aaatcacatc acatccatta tagtagccca
     2581 tctttataaa ctctctttca ttattactaa agtggcattc aatagatagg cgagagtgca
     2641 tatcacctaa tataatatga ttgccatttg atttttctga tatatatatg tgtaaattat
     2701 cctgtagtgt ggcgggaacg aaataggtat tgctccctcc tccagaatat ataacacctc
     2761 ctccactccc aagagaaaag gtattatcaa gattattacc acgaatctta tcatatccca
     2821 gtttagtacc aacaaccgca tttataccct tttgcgttat gctcataact tttcctgaat
     2881 aaaaacctga gggatgatga aaattaaaat tttgtttcct cccatctgat aaatccaaat
     2941 ccagattgaa gtcacgtgat aattgttcaa acgatataat atttctaaca ccgggggtac
     3001 attctatgtt gtaatcacca ataccaccaa ccattaattt tatccctccc ttccctcctg
     3061 atatcgagaa attatagttt ttaaaacgtt caatcatatg tagattagag ccaagcatac
     3121 catgttcaat agcgggaata ataattgtta atggttcttt tccaccaata acatgactat
     3181 gttggttaca tactgttaat aatggctcag atagttcatc atcagtatat gcccttgcaa
     3241 tctcgtaaaa gtaaccagtg ctactcaatg taacttcagt attagctcgc aatgtttctc
     3301 catatccaag atatactgta ttataaattc cttctggaat aactggaatc tgagaaggcc
     3361 attggcggtt agaataccca ttagctaatt gtgattctga taattccatt gaattacata
     3421 tttccttatc tccccaaccg aaaatatcgg gttcacctgc atctgagagt tttgtgcatg
     3481 caaaattgta tgctctagac tccataatct ctctgtcagt catatcagga tgactaccaa
     3541 tattctttcc gctattatat gatttctcac cgtgtaatat tggaggattt tctctcatat
     3601 ctaaataaat attacctaac acctgattat ttgataaatc gattatgcct gtggctttgt
     3661 taatgcttag aacctttgca cttccattgt ccagatactt ttcagcttcg agccataact
     3721 tatggcgctc attaatttga ttcacataaa cagcatagtt ttttacttca tgagaaaaaa
     3781 tagttatcgg aactatagct attgccaaag gaggatatgc gattgcgcct attgacaaag
     3841 ctgtatttat agatgaaaaa gatacatcta ttgctgttga tatatattca atttgtgtgg
     3901 tggctttagc atgatttaat gaagaatcat ataatgacca aatatttatg cctatatcta
     3961 gcaaaggtaa tttaattact tttgccacat tgctaagata acgccctgcg gtgccaccca
     4021 ttcttgaagc acacacctcc aacccagaag atataaatcc ttcaagcgag gcaccagata
     4081 caccgccagg attatatacg cgattattag taagagtcaa aattccgcct aaaaatttgt
     4141 ctacaatatt ttttatatct gatactgctc ctgcatgatc tattgcacta atattgtcat
     4201 tcatttttat cattcgtgca tattgaacca agccaataac agacataatc gcatcgatat
     4261 tcatgtgttc gagtgcttcg gataaactat ttatcccctc tttaaaagtt aagccaataa
     4321 ctcttgtatc tatatcaaca cgataaagtc ttccttgcag atctgttact gtaacctcca
     4381 ctgtatcact atttatcttt gattctttga agttattaac tgaaagaaca tttgttttaa
     4441 gtgaaagacc ttgctttctt aacttatttg aaaactcatc aagcaatcca gatagtaaat
     4501 tctttacgtg ttctttctgc cgtgaaagaa tggaatgaag gggctgttgg atttcactta
     4561 taggtttaat atcactttca agcttaactt ttattgtgtt atcctgaata cctaaaaaat
     4621 ccacaatatc ttttatcttc tgagtttgct cctctgacaa ataatgcgta tttatataat
     4681 gttgtaaaat atccccattt atttttaagc tcctaatttt atttttcaca tctgtacttt
     4741 cacttattct gttaccatta ataactcctc cgatgtcata aataaaactg acaggcacga
     4801 gatgccccat aatatttaat gaatccccgc ttgtggataa tttttctggg gttgttaaat
     4861 atatgtctct taacaaagaa ccagtctgaa caagggggag cttaatatac ttccaattat
     4921 taagagattc actgaagtaa attttaacaa tgccacttcc atataaagat gaaaagttac
     4981 gattgctcgt tatatttttt ataaacgtta aagcctgtgc taaggaatga aagtcacaat
     5041 gaccaaaatt aggatcagtt actctccaaa aagaagatat ttgttgtaat gcaagtgaat
     5101 gatattctgt tgttattaaa agagatgata tacgacgctg gtataatatg ccttcagtta
     5161 gtatagggtc tttcaattcc agaacctcta actgtgcatt ttttaacctg ttattaccta
     5221 actcattgga cattgtaatg atacgctgtg tttttcttaa aaagaaatca tcgcggttag
     5281 ataattttag attgtcattg tattttgttt gatataatgt gctggcagtg tcaatattgt
     5341 gcctcagttt ttgatatccg cctccgtacc cccctgtatc aagatacaga agagataaac
     5401 caaggcactt tcctttcgaa tctctatcaa aaagaagact ttgtgggtga aattcgatat
     5461 ttgtactttt atgctgtctt gctattgtat cccatattga cgcatgagtg ttataaaaat
     5521 cattccatga aagtaaatta tattttgctt gttcaacaat tgtatgcaat gcaggcacat
     5581 cagaaacgga tttatctata ctatttatgg caccaagatt gctaaagtca tatggtttaa
     5641 ccactgaagc aacagtcata gcatttgtta actgatgccc ataatattct gtttttttta
     5701 ttattgttgt tatgctggga catatggtct gcaataatgg tatagaaata tctgcaaatg
     5761 aagtactatt tgttaaagtt ctatgtattt cactcagcga taaatattta tattttttta
     5821 ttaagccttc aagattcaaa actctctcgt cattaatcaa aaaatccagg tttaaagatg
     5881 aaaacttact atatataaac tccctgtttg ccttgttttc tattattcca atgtgaatgt
     5941 catatagcat ctcgaagtag cgatacataa aaggcagcgt taaattttga tttataggta
     6001 aagacaaaaa caaattctca ttgtatctgt caaaatcaac agaatattta ataccaataa
     6061 ctgatgattt ttgctctgtc aacttcatga aatactgttc tgatacagat ttactaattt
     6121 ctaattttat atcattcaat atttttttct gttcttctga taaagggggg cgcaagacgt
     6181 tggatacatt atcggataat atatcataaa catatggggt tattttttct atccactcag
     6241 aattgctctt tgtatgtctt cttaatagct caagtaattt atcagatgga gctggatata
     6301 ccacgccgtg agaataatgt ccagaaactc cgtaactagc caataagtat tctgcaatta
     6361 tgttgatatc agaaaaaacg tagccactta attgtaattt ttctaataaa ctacccggat
     6421 atgcatagtg ggatatatcg agaaatgcta ttcgctcttt aatatcacta tctaactgat
     6481 ggtaaagtac gtttcgtttt gtttcaggat ctgttatata tttgtttttt atatcatcga
     6541 aaattgactt agccgaagat aattcacttt tcacataaaa ctcaaatttt ttaaattgct
     6601 ctaccgttga taaatccccc cactcatgtt gattgcttcg tattatatca aatgattcat
     6661 gacccgatat aatcgaattt tcgatgttgt atataatgcc tgatagttta tccctctctt
     6721 taatatatgc atttgtagtt atgatttttt tataaccatc atcatctaac atcgaaaata
     6781 gttctttcat ttttaatttt gcataatctc ttaaagctcc ggcatcctcc ttagataagt
     6841 tttttttgtt ttcatttata taactatcta cttttttact tcgtagaaca catgatataa
     6901 cctctcggag aaacacattt ttttccatta cggaattgta ccagacaata atttttgtat
     6961 cggcatcatt acgcgaaaat aattttattg cgctgagtaa gtcatcttgt ttttttctat
     7021 aaaaatcata aataaaatta attgtgtttt caggttcaaa tgtattcgaa gttacgtaat
     7081 tagctaattg tagctgtaag gttcttatag agcgcaaggc caacggatta tctattttgc
     7141 tgctaatcgc atataattca cgatccagtt ttattaaaag tttaacatca atattagacg
     7201 atgttaataa taaattaata tctttgatga ggtctttatt aatctctgta acatgcttta
     7261 tgcttttatt atcccacccc tgaaaaaaag accgcgaatc gatttcatag tatagggagg
     7321 taggtagttt ttcgggtaca tggcagttta ttttttcgaa tagaatttta tttatatcat
     7381 tatttctgac agcccatgag ctttcatcat ctggtgtaat atcattaata ccaacactca
     7441 ctggatttag ccagttaaaa cttttatttt ctttatttga acttgcatat acacccaaaa
     7501 aattaacatc actcaattta ttatctttaa tatgcacttc acctatgcgc ccaagagatt
     7561 tatagtattt tttcatttgt cgcataatca tgtctgggcc agatatattt aaagtgctaa
     7621 ccacataagg aattgtaccg tcctgtctat attcagaaaa caaaccgtgt tctaaatatt
     7681 tctttgcagg tattcctcct attgccacat tatttaaaga ctttagatcc tctgtagtat
     7741 aaaatagatt attataactt atattatctc tgatgcgttg gagttctaaa tactgttttt
     7801 tctgtcctga tagaataaaa tcaatacatt tacttccctt gtgagttaac atgaaattat
     7861 tattccctct aatattccag gtgttttctg tccatttttg atatctttta aaaattttaa
     7921 atgtatctct gacaactttt gtctcaacgg gtttaaaata atcatctatc gtcagttttt
     7981 ctatttcact caatatgtta tttaactgat ttagtgttgt ttcatctaat ccatcatgtt
     8041 taattgagta cttttcccct tttatcagag acagaataga ttctgatata atccttctta
     8101 gttttaaatc ttcaaaaaac ctcttatcat cacttttttc atttattatc tgtgatactt
     8161 tatctgaata tgcaggaaga atatctgtat cggtataaat ccctccatac tctttcaata
     8221 tattaattct tacctgatct gatgcagcgg cgtagttcca tcttaatagc atctcttgtt
     8281 gataaaaaaa ataatcttgt ggttttttaa atgactccaa tgtgttaata tctctcagtt
     8341 gaaatctatc cccaaatttt tgagatattg tattttttaa taagtctata tctcttttgt
     8401 ttttctctac tgtagattta aagtcatttt gaacttctgg tgaaagtttt aaaacgttag
     8461 ttaaaaagtc aagtctggat atatcatcag tatttgatat tccgtttgat atacaatatg
     8521 taaaatagtt gtcttgcata cctatcattg actcaagaaa aaaaacattg aatttttctt
     8581 tagtttcact cgttaatgat ttatatctat tttcaagttc cttcaatctt tctcttgttt
     8641 ccttaaattc tatagtttca ttttgtatgt tttgtatttt taatatcact tcattcattt
     8701 cttccgctaa atgtggattc gttcttctca aacgcataat tgcataattc atcgctatat
     8761 tttttaaaat cccactaaac ttggccgcac caaaggcatt aggatcaatc cagagcaggt
     8821 aggtataatc tttatatgta tttaaaaatg ccgttatata tgaaaatgta ttatcaggag
     8881 gtgcgccagc aacccatact gcatgtataa atggctctat gtctacttta tgtgactgat
     8941 actctatacc caataagttt attattttag atgcattgtc tgataattgt ttatttcctt
     9001 tattatcatt tattaatgat gataacagct ctccctgctt aacaagtatt tctatacctt
     9061 ttcgactatt tttaatttgc atattttcat atgaatgtat gatatcattg agctcactta
     9121 ttttctgaaa agggatggta tttctatcaa ctccatttaa caactcatta attatcaaac
     9181 tttgagtgat agttgttgtt tgatcttcag cgcctcttgt ttttcttatt ttaatgtatc
     9241 cgtcgatgtt attacttgtt ggtaaatgtt tgatactctt ttgttgtaat agttcctgct
     9301 tttcaggctg atgtatagta agtagagtag aactggggga tgaattattt tcaccctctc
     9361 gggaacaaaa atagttacta ccattaaata tgctatatag tagtctagca agattacata
     9421 aaaaagatgt tttccagttt cctgagttac ttacgatatt tcgatcatta tgtatggttc
     9481 tgtaacttct aacttcatct aaaatcattt tgcctattaa aacaacatct tctgtcgcat
     9541 ttttctgctt tacgagcgaa ctgtaaagtt taaatctgga tgagatatca tcattttttt
     9601 tatagataaa gttccttttt ctctcttgaa tttggccatg tttaacatca gtactaacat
     9661 ttttaactct tgtattgtta attgctttat ctaaagaaga gccaggatga atcatatcta
     9721 taccctctaa ttatgtaaca cttaataatt tttgattagc gacaacctaa aatgtatttt
     9781 gatgttgtta tataaattaa tgtattcggt ggtgcatcca attagtagaa catgtgtttt
     9841 tcgataaacg ctccgatcac tttttcgtgg atctcaactg agcgtgagaa gcagattgtt
     9901 ttacgagtca accgcttaat gcgggtgcgt agcgtcagat tattacgctc aatgcgttgg
     9961 gtgaatattt tgccagcacg ttatcaccga acatctctcc cccagtcggc gaacccttta
    10021 ttggacgtca gcacaatgct cgctttttca tatcgacggt tcagcaaccg gaagaacaga
    10081 ctgtgttcct gcgtaaagcg ctgctccggt gtcttcctga actggcgaag caacatggaa
    10141 cggccaccgg tataacccat cgctttgatt tccgccagga taacttcgcc gttccagaca
    10201 ttctcagcca gacgcatgtc gatatagtcc atttcggcca ttttgtggcg tgtttttctg
    10261 gccggaggtt ctgggtattt caggtaacgt ctgaccgttc gttcagagca accaatctga
    10321 gtcgcaatat caacaatata cgcaccttgc tggcgcattt gctttatcat gtaaaagtcc
    10381 tctctgctca gcatgctgat gtcctttctg gtgtgagaac ctcaaggaaa caacatgttg
    10441 ggtggagcgg acaatccaaa tggtgaatta ccgtcttata tcactggcgg ataaggataa
    10501 gtacacgtcg gcagttccgt cagcactgcg acagcgttgt cctcgttgct tttacccggt
    10561 catgcacaac gcctgatgga tgaactgcgt gcccagggtt accattttaa agtaaaaacc
    10621 gtgacggaaa gcctgcgccg tcacggactg agggcaaaag cttcctggaa tttcagtccg
    10681 gtctgttacc gcgcacacag ccagcctgtg tcagaaaatc tgctggagca ggatttttac
    10741 gccagtggcc cgaaccagaa gtgggcggga gacatcacat atttacgtac ggatgaaggc
    10801 tggccgtatc tggctgtggt cacgtgccat tattggctga tcaatgtcgt cacgcatgac
    10861 ggcacaggag accccggaac gttatgctac cgcatgtcat ttgcaaaaat gacagataga
    10921 tatacttcaa tttgaatctt aacaattagc atgggaatgt tgtcaatcct ctgtatggag
    10981 ccaccacata ttgcaatttt tgttctcttt taaaatatat tccacgagcg tcttgtggga
    11041 ccttttgctg ttataggggg gcagtttgga tatcgaaaat tattgtacgt taaggctgaa
    11101 tttcaatcgc aaagcataac cttacctttc tgcttactta tgcagttcaa agagtaccaa
    11161 ctctgcaatt ttcgttgcca cagatgcaat atcgttactt tccagtcacc atgccgctga
    11221 aagcccacac catgcga
//
LOCUS       DAZKHC020000121        11115 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZKHC020000121 DAZKHC020000000
VERSION     DAZKHC020000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.121"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            44..145
                     /gene="ykgR"
                     /locus_tag="ACHOSM_RS19945"
                     /old_locus_tag="pgaptmp_003989"
     CDS             44..145
                     /gene="ykgR"
                     /locus_tag="ACHOSM_RS19945"
                     /old_locus_tag="pgaptmp_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HGU4560386.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            509..772
                     /gene="ykgM"
                     /locus_tag="ACHOSM_RS19950"
                     /old_locus_tag="pgaptmp_003990"
     CDS             509..772
                     /gene="ykgM"
                     /locus_tag="ACHOSM_RS19950"
                     /old_locus_tag="pgaptmp_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGU4560387.1"
                     /translation="MKPNIHPEYRTVVFHNTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
     gene            772..912
                     /gene="ykgO"
                     /locus_tag="ACHOSM_RS19955"
                     /old_locus_tag="pgaptmp_003991"
     CDS             772..912
                     /gene="ykgO"
                     /locus_tag="ACHOSM_RS19955"
                     /old_locus_tag="pgaptmp_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HGU4560388.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KP"
     gene            complement(947..1174)
                     /gene="ykgL"
                     /locus_tag="ACHOSM_RS19960"
                     /old_locus_tag="pgaptmp_003992"
     CDS             complement(947..1174)
                     /gene="ykgL"
                     /locus_tag="ACHOSM_RS19960"
                     /old_locus_tag="pgaptmp_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HGU4560389.1"
                     /translation="MQAAPQREETEWRVQSKRELMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            1997..2539
                     /gene="ecpR"
                     /locus_tag="ACHOSM_RS19965"
                     /old_locus_tag="pgaptmp_003993"
                     /gene_synonym="matA"
     CDS             1997..2539
                     /gene="ecpR"
                     /locus_tag="ACHOSM_RS19965"
                     /old_locus_tag="pgaptmp_003993"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HGU4560390.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            2614..3201
                     /gene="ecpA"
                     /locus_tag="ACHOSM_RS19970"
                     /old_locus_tag="pgaptmp_003994"
                     /gene_synonym="matB"
     CDS             2614..3201
                     /gene="ecpA"
                     /locus_tag="ACHOSM_RS19970"
                     /old_locus_tag="pgaptmp_003994"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HGU4560391.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            3259..3927
                     /gene="ecpB"
                     /locus_tag="ACHOSM_RS19975"
                     /old_locus_tag="pgaptmp_003995"
                     /gene_synonym="yagY"
     CDS             3259..3927
                     /gene="ecpB"
                     /locus_tag="ACHOSM_RS19975"
                     /old_locus_tag="pgaptmp_003995"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HGU4560392.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            3953..6478
                     /gene="ecpC"
                     /locus_tag="ACHOSM_RS19980"
                     /old_locus_tag="pgaptmp_003996"
                     /gene_synonym="yagX"
                     /pseudo
     CDS             3953..6478
                     /gene="ecpC"
                     /locus_tag="ACHOSM_RS19980"
                     /old_locus_tag="pgaptmp_003996"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
     gene            6468..8111
                     /gene="ecpD"
                     /locus_tag="ACHOSM_RS19985"
                     /old_locus_tag="pgaptmp_003997"
     CDS             6468..8111
                     /gene="ecpD"
                     /locus_tag="ACHOSM_RS19985"
                     /old_locus_tag="pgaptmp_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HGU4560393.1"
                     /translation="MRVNLLITMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYMQQMPVGSSFTMTINACQTSVNYDANSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            8080..8790
                     /gene="ecpE"
                     /locus_tag="ACHOSM_RS19990"
                     /old_locus_tag="pgaptmp_003998"
     CDS             8080..8790
                     /gene="ecpE"
                     /locus_tag="ACHOSM_RS19990"
                     /old_locus_tag="pgaptmp_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HGU4560394.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            9103..9432
                     /gene="ykgJ"
                     /locus_tag="ACHOSM_RS19995"
                     /old_locus_tag="pgaptmp_003999"
     CDS             9103..9432
                     /gene="ykgJ"
                     /locus_tag="ACHOSM_RS19995"
                     /old_locus_tag="pgaptmp_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU4560395.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(9680..9805)
                     /locus_tag="ACHOSM_RS20000"
                     /old_locus_tag="pgaptmp_004000"
                     /pseudo
     CDS             complement(9680..9805)
                     /locus_tag="ACHOSM_RS20000"
                     /old_locus_tag="pgaptmp_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10274..>11115
                     /locus_tag="ACHOSM_RS20005"
                     /old_locus_tag="pgaptmp_004001"
     CDS             10274..>11115
                     /locus_tag="ACHOSM_RS20005"
                     /old_locus_tag="pgaptmp_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HGU4560396.1"
                     /translation="MKSETLTVQQLFQDRRQYCVPFYQRAYVWTQRNQWSALLEDILE
                     KAQSRLSGTKPTPHFLGAVVLEPQPKKGLLGVDSIHIIDGQQRLTTLQYVLASIRLAL
                     RATDLSNLEPLISSCLKNSNEDTMRNKEVERFKLWPTFRDQTHFIQSFNVENIDDIRN
                     VFSDSFTKHGTLRKHFNHPPSLEALCFFTEAFIKWIKIENHSPRENAVALIEAVLTDL
                     KLVSIFLEAEDDAQIIFETLNGRGAELHATDLIRNYIFMCAEHENINAVELYENEWKI
                     FEDT"
BASE COUNT         3055 a         2713 c         2624 g         2723 t
ORIGIN      
        1 ggcacttacg aagtgcgctc ttttttaaag cgaggaagta ccaatgaaag agaataaagt
       61 acaacaaatc agtcataaac tgattaatat cgttgttttt gtcgcaattg tagaatacgc
      121 ctatttattt ctccatttct attaataacg gcaataaact gttcacttca gtgatattta
      181 aaatatgcat cctctccctt tttgtaagta attattatat ccgtgggaga ggaatacacg
      241 ctgtcaggta atcaatcata ctgcgataaa tcatcggcca gtaaagtgga gataacctcc
      301 attgtcgaaa aatccatact ctcagcgaaa ccatcatcaa tcactcatcc aggcgtttat
      361 gggagcgtcg ccaacggctg ctaacaatgc cagacttccc cgttggggaa attccacata
      421 ccaaaaatgg gcacagggat tgggtgttga aatgatccgg atgagcatgt atctttactg
      481 ttaagttata acataacagg taaaaatgat gaagcccaat atccatcctg agtatcgtac
      541 tgtggtgttc cacaacacca gtgttgatga gtactttaaa atcggctcga ctatcaaaac
      601 agaccgtgag attgagctgg atggcgtaac gtatccgtac gtgacaattg atgtctcttc
      661 taaatcgcac ccgttctata cagggaagct gagaacagtg gcatcagaag gaaatgttgc
      721 acgattcacc caacgttttg gtcgttttgt tagcacgaaa aaggggtcgt gatgaaagtt
      781 cttaactctc tgcgtaccgc aaaagaacgc catccagact gtcagattgt gaagcgaaaa
      841 ggacggctat atgtgatttg taaatctaat ccacgtttta aggccgttca gggtcgtaag
      901 aaaaaacctt gattcaaaat tcgacggatt aacgatattt gtctgattaa taatcagatc
      961 ggattaatgt tggtgtgttt ataacaccaa cattaatttt cctggggata tattcttcct
     1021 gttcatttga ggccaactgc ctgacgtttc tctccgaata ttccattatc ttaatgttga
     1081 cttgttgacc agcttcgccc ctgtatgctg gcatcaactc tcttttagac tgaacacgcc
     1141 actcagtctc ctccctttgc ggcgcagcct gcattttcac tcaaactgtt aagatgataa
     1201 atgtggtaaa tctgttggta ctaacataaa aacgtttagg ccacaggaac agtctgttcc
     1261 accggtaacc ccgtcgccga cgttcgagtg gcagttagag taacgcgcac agataactgc
     1321 actcagtgcc ctgacaaaga ggccatcgtt cctgtgacag ctggcagcct tcgtttaact
     1381 tcacttaatc tggctcttgg gggcttaccg aacagatgac gtacatacgc ccgttcaatt
     1441 ttccatcact tattgtaatg aacacctata accattttgt gcggcatgtt aatccattaa
     1501 aatttcttac tgattggcaa atcatcttca atgacagctc attatagttt tatattctat
     1561 cccttaccct taaaacttgt ttttttacta gtccatcaca cagcgcatta agactattcc
     1621 taacacttca gggcaaagtt cctgaccaat ataaaatgca agtaagaatt gaacgttata
     1681 ttgccaataa ccttatgaaa ccaaatgtct ttttcttctt atcaaaaaag caatattttc
     1741 agtttttcta aatattgact taaccattga attccttttc cgttcacata ttgacactca
     1801 tcgggaaaaa aacataaatt taagcccaat cgaaaataat taaacttaat ctcgtttaac
     1861 ctttattgat atgtgctacg tatcttattt acttccggtt tactaaggaa actgaatgca
     1921 cctgtaaaaa ttacaggttt ggaaagtagt gacatggcaa agtgattaca gcagggacta
     1981 tgaggttaaa aaccatatgg aatgtcaaaa ccgttctgat aaatacatct ggtctcccca
     2041 tgacgcctac ttctataaag gactatctga actgattgtg gatatcgaca gattaattta
     2101 tctatcgttg gagaaaatta gaaaagattt cgtgtttatc aatctcaata cggattcttt
     2161 atctgaattt ataaaccgtg ataatgaatg gttatccgcg gtaaagggga aacaggtcgt
     2221 attgattgcg gccagaaagt cagaagcctt agcaaattat tggtattaca atagcaatat
     2281 taggggcgtg gtatacgctg gactgagtcg tgatattaga aaagaactgg cctatgtgat
     2341 taatggcagg ttcctgagaa aagatattaa gaaagataaa atcacggacc gggaaatgga
     2401 aattatccgc atgacggccc agggaatgca acctaaatcg attgccagaa ttgaaaattg
     2461 tagtgtgaag acagtgtata cccatcggcg taatgctgag gccaagctgt actcaaaaat
     2521 atataagttg gttcagtaaa ctccaggcaa gttagtttta aaaaatgact cactgggaca
     2581 tcacgtcctc aattcaactc gggaagaaat gcaatgaaaa aaaaggttct ggcaatagct
     2641 ctggtaacgg tgtttaccgg catgggtgtg gcgcaggctg ctgacgtaac agctcaggct
     2701 gtagcgacct ggtcggcaac agccaaaaaa gacaccacca gtaagctggt tgtgacgcca
     2761 ctcggtagcc tggcgttcca gtatgccgaa ggcattaaag gttttaactc acagaaaggt
     2821 ctatttgacg tggctatcga gggtgactca acggctaccg cctttaaact gacctcacgt
     2881 cttatcacca acacattaac ccagttggat acctcaggtt ccacactgaa tgtgggcgtg
     2941 gattataacg gcgcggcagt cgaaaaaact ggcgataccg tgatgatcga taccgccaac
     3001 ggcgtactgg gcggcaacct tagcccgctg gctaacggtt acaatgccag caatcgtacc
     3061 accgcacagg atggtttcac cttcaccatc atcagcggca ccaccaatgg taccaccgca
     3121 gtaaccgatt acagcactct accggaaggc atctggagcg gcgacgttag cgtacagttc
     3181 gacgcgacct ggaccagtta atctctctga tgtaccagca ggggtagccc ccctgcttta
     3241 ttcctggacg gaattgttat gaaaaagcac cttctgcctc tcgctctgct gttttccgga
     3301 atatctccgg cccaggcgct ggatgtcggc gatatatcat cgtttatgaa cagtgacagc
     3361 agcacgctga gcaaaacgat caaaaacagt accgacagtg gtcgccttat caatatccgt
     3421 ctcgaacggc tctcttcacc gcttgacgac gggcaggtta tctcaatgga caagccggat
     3481 gagttgctac tcactcccgc cagcttgctg ctacccgccc aagccagcga agtgatccgc
     3541 ttcttctata agggacccgc agatgaaaaa gagcgctact accgcattgt ctggtttgat
     3601 caggccctca gtgatgcgca gcgcgataat gccaaccgca gcgctgtggc cactgcttcc
     3661 gcccgcatcg gcaccattct ggtcgtcgcc ccccgccagg cgaactacca ctttcagtac
     3721 gccaacggct ccctgacaaa tacaggaaat gcgacgctgc ggatcctcgc ctacggacct
     3781 tgcctgaaag ccgccaacgg taaggagtgt aaagagaatt actacctgat gccgggcaag
     3841 tcgcgtcgtt ttacccgcgt ggacacggcg gataacaaag gacgggttgc actttggcag
     3901 ggtgataagt tcattcccgt gaaatagata gctgtgcaga tggataacga caatgccttt
     3961 acgacggttc tccccaggac tgaaagccca gtttgccttc ggcatggtct ttttgttcgt
     4021 tcagcccgat gccagcgctg ctgacataag tgcgcagcaa ataggtgggg tgattattcc
     4081 gcaggccttc agtcaggcgc ttcaggacgg catgagcgtc ccgctctata ttcatctcgc
     4141 cggtagccag ggtcgccagg acgatcagcg aatcggcagc gcttttatct ggctggacga
     4201 tggacagcta cgcatccgga aaatacagct ggaagagagt gaagataacg ccagtgtcag
     4261 cgaacaaact cgacagcagc tgatggctct ggcgaacgcc ccgttcaatg aggcccttac
     4321 catccccctg actgacaacg cgcagctgga tctcagcttg cgccaactgc tgctgcagct
     4381 ggtggtcaag cgcgaagcgc tgggcaccgt actacgctca cgtagcgaag acatcgggca
     4441 gtccagtgtt aacaccctca gcagtaatct gagctataac ttgggcgtct ataacaacca
     4501 gttgcgtaac ggcgggagca acacatccag ctatctgtcg ctgaataacg ttactgcact
     4561 gcgcgaacat catgtggtgc tcgacggctc gctgtacggg atcggtagcg gtcaacagga
     4621 cagtgaatta tataaagcga tgtatgaacg cgattttgcc ggtcaccgat ttgccggtgg
     4681 aatgctcgac acctggaact tgcagtcctt agggccgatg accgccattt cagcagggaa
     4741 gatttacggc ctttcctggg gaaaccaggc cagctccacc atcttcgaca gcagccagtc
     4801 agccacgcca gtgatcgcct ttttaccggc ggcgggcgaa gtacatctca cccgtgatgg
     4861 gcggctacta agcgttcaga acttcactat gggcaatcat gaagtggata cccggggtct
     4921 accgtacggg atttacgatg tggaagttga ggtgatcgtt aacggtcgcg tgatcagcaa
     4981 acgcacccag cgggtcaata agctgtttag ccgggggcgc ggcgtcggtg caccactggc
     5041 gtggcaggta tggggcggta gctttcatat ggatcgctgg tcggaaaacg ggaaaaagac
     5101 gcgaccagct aaagagagtt ggctagcagg tgcctcgacc tccggctcat tgagtacgct
     5161 tagctaggcg gcaacgggat atggatacga taatcaggcg gtgggtgaaa cccgtctgac
     5221 gctgccgctt gggggggcga tcaacgttaa cctgcaaaat atgctggcca gtgacagctc
     5281 atggagcagc atcggcagca tcagcgccac tctaccggga ggctttagtt cgctgtgggt
     5341 taatcaggaa aaaacccgca ttggcaatca attgcgacgt agcgatgccg acaaccgtgc
     5401 aatcggcggc acactcaacc tgaactcact gtggtcgaag ctgggcacat tcagcatcag
     5461 ctacaatgat gaccgccgtt acaacagcca ttattacacg gcagattact atcaaaatgt
     5521 ctacagcggt acctttggtt cgcttggcct gcgggccggt attcagcgct ataacaacgg
     5581 cgacagcaac gccaatacag gaaaatatat cgctctcgat ctctcgctac cactgggcaa
     5641 ctggtttagc gcagggatga ctcatcaaaa cggctacacc atggcaaacc tgtcagcacg
     5701 caaacagttt gatgaaggaa ccattcgcac tgttggtgcc aatctgtcac gtgccatctc
     5761 cggcgatacc ggtgatgaca aaaccctcag cggtggggcg tatgcacaat tcgacgctcg
     5821 ttacgccagc ggaacgctga acgtcaatag cgcggcggac ggctacgtca ataccaactt
     5881 gaccgccaat ggcagcgtcg gctggcaggg taaaaacatt gctgccagcg ggcggactga
     5941 tggcaacgct ggggtgatat tcaacaccgg gctggaggac gacggtcaga tcagcgccaa
     6001 aatcaacggg cggattttcc cgcttaacgg caagcgtaac tatctcccgc tctctcccta
     6061 tggaagatat gaggtggagt tacagaacag caaaaactca ctcgacagtt acgatatcgt
     6121 cagcggccgc aaaagtcgtc tgactctcta tccaggcaat gtcgctgtca ttgagccaga
     6181 ggtgaagcag atggttaccg tctccggtcg tatccgtgcg gaagacggca cactgctggc
     6241 taacgcacgg attaacaacc atatcggccg aacccgaacc gatgaaaacg gcgagtttgt
     6301 catggacgtg gataagaaat accccactat cgattttcgc tacagtggca ataaaacctg
     6361 cgaagtggct ctggaactca accaggcgcg cggtgccgtc tgggtcggtg atgtggtctg
     6421 cagcggcctc tcatcgtggg cggcggtgac gcagacagga gaagagaatg agagttaacc
     6481 tactaataac gatgataatt ttcgcgctaa tctggccagt aactgcgctc agagcggcag
     6541 tgagcaaaac aacctgggcg gatgcaccgg cacgcgagtt tgtgtttgtc gaaaacaact
     6601 cagacgacaa ctttttcgtc actcctggcg gggcgctgga tccgcgcctg accggtgcca
     6661 accgctggac cggtttaaaa tacactggtt caggaaccat ctatcagcaa agcctcggct
     6721 acattgataa cggttacaac accggccttt ataccaactg gaagtttgat atgtggctgg
     6781 aaaattcacc agtttcatct cctttaactg gcttgcgctg catcaactgg tacgctgggt
     6841 gtaatatgac caccagtctt atcctgccgc aaaccaccga cgccagtgga ttttatggcg
     6901 cgacggtgac cagcggcggc gcgaagtgga tgcacggcat gttgtcagac gcgttttacc
     6961 agtatatgca acaaatgccc gtcggcagca gctttacaat gaccatcaat gcctgtcaga
     7021 cctctgtgaa ctatgacgcc aacagcggcg cgcgttgtaa ggatcaggcc tccggcaact
     7081 ggtatgttcg caacgtcacc catacgaaag cagcaaatct acggttgata aatacccact
     7141 cgctggcgga agtatttatc aacagcgacg gagtaccgac tctgggcgaa gggaacgccg
     7201 actgccggac gcaaaccatc ggcagccgtt caggattaag ttgtaagatg gttaactata
     7261 ccctgcaaac aaacggactc agcaacacct caatccatat attcccggcg atcgccaact
     7321 cgtcgttagc ctcggccgtc ggggcgtacg atatgcagtt cagtctgaat ggcagttcat
     7381 ggaaaccggt gagcaatacc gcctattact acaccttcaa cgagatgaag agcgcagact
     7441 cgatctatgt tttcttctcg agcaacttct ttaagcagat ggtgaacctc gggatcagcg
     7501 atatcaacac caaagatcta ttcaactttc gctttcagaa caccacatca ccggagtctg
     7561 gctggtatga attttctacc tccaacacgc tgattatcaa accccgtgat ttcagcatca
     7621 gtattatctc cgatgaatat actcagacac cgtcgcggga gggatatgtt ggcagcggcg
     7681 agtcggcact cgatttcggc tatatcgtaa ccaccagcgg taaaacagct gccgacgaag
     7741 tgctgatcaa ggtgaccgga cccgcgcagg tgattggcgg gcgctcctat tgtgtcttca
     7801 gctccgatga cggtaaggcg aaagtaccgt tcccggcgac actttccttt attacccgca
     7861 acggagctac aaaaacctac gatgccgggt gcgatgatag ctggcgggat atgaccgatg
     7921 cgctgtggtt gaccacaccg tggactgata tctctggcga agtggggcag atggataaga
     7981 ccacagtcaa attttcgatt ccaatggata acgccatttc gctgcgtacg gtagatgata
     8041 acggctggtt tggcgaagtc agcgcttcag gagagattca tgttcaggcg acgtggcgta
     8101 acattaacta aggccctgct gacagcggtc tgtatgctgg cggcaccttt gacacaggcg
     8161 atttcggtcg gcaatctgac attttcgctg ccgtccgaga ctgactttgt cagcaaacgt
     8221 gtagtgaata acaacaaaag cgcacggata taccgtattg ccatcagtgc tattgatagc
     8281 ccgggcagca gtgaattgcg cacccgaccg gtggatggtg aactgctttt cgccccccgc
     8341 cagctggcgt tgcaggctgg tgagagcgag tattttaaat tttactatca tggtccacag
     8401 gataaccgcg agcgctacta ccgggtctca tttcgcgaag tccccactcg taaccagaca
     8461 agacgtagcc caaccggcgg cgaggtcagc acggagccgg tggtggtgat ggataccatt
     8521 ctggtagtac gaccacgtca ggttcagttt aaatggtcct tcgacaaggt gacaggaacg
     8581 gtaagtaaca ctggcaacac atggtttaag ctactgatta agccaggatg tgattcgacc
     8641 gaagaggaag gcgatgcctg gtatctacgt ccgggagacg tggttcatca gcctgagtta
     8701 cgtcagccgg ggaatcatta tctggtctat aacgacaaat tcattaagat tagcgattcc
     8761 tgtccggcta agcccccttc ggcggactaa gacttatcca tcggtcgagg gaagaattcc
     8821 acctcagagc tccaaaaatc gtttaaatga tgtggaagcg atcgtcaatg tcacctgtaa
     8881 cgatgggctt tctcatatgc acaccatagt gttcaggaga cacggaaagg tatcaggcag
     8941 cctgctaacg agtggtttaa tcacgaatta gtacgtaaaa tcggtaacgg ctggaaatca
     9001 ttcaatactc acactatcga aagttcccct gccaaccgcg gtacgttctt acatacgatg
     9061 taccgctgtt ctctttacga tttttagctg tactggtgaa ttatgagcaa tctgaatcca
     9121 tgcatgacgt gtggtgcctg ttgtgcattt ttccgcgtct ctttttactg ggccgaagcc
     9181 gacgatgctg gcggcactat tcccgccagg ctcactgaac aaatatcccc ttttcaccga
     9241 tgcatgagcg gtaccaatca gaaaaacccc cgatgtattg cccttgcagg aaccccgggc
     9301 aaaaatgcct gctgcacgat atataaaaat cgatcgtcca catgcagaga attcgccatg
     9361 tctggtgaga acggagtcgt caatgaggct tgcaatcgtg caagggctaa atacgggctg
     9421 acaccgttat aaacatacaa taattaattg cactgccccg ccagcgataa tagcggggct
     9481 tcgtttttca ggggtaacaa aacccgatac ttcttctatt tgccagcaac aatgcctctc
     9541 ttctgtagcg ttcttcggat ctacatcatc ctgaagtagc gcgatatact cactgactat
     9601 ctgcatcaga atataaaaag caatgttttt aacctataaa aatggcgctg tatttgcgcc
     9661 atttttatca ttcaatgcat tatctgtttg agcctaaagg gatctcaggg tctggctcat
     9721 gagtaattct gtttcgtaaa tctctgcgaa taatttcaat agaccagaac cagactaaat
     9781 gtccaaaaat ttcagaaaca ttctcagccc ccctcaactt ccgcttttgg cacatagcag
     9841 ccctagagac agtggcgtaa agtcatggag ggtcggtggc aggagatgca aatcctctca
     9901 tacaaaaata cgtaaaatca ataacggcta gaaatcattc aatactcgca ctatctaaca
     9961 tttaccagcc aaccgcagca cgtcttgcat acgacctgtc tgcggtttca atcacctctc
    10021 accaccagtc ccatcaataa taattaaaca gcgcctaaac aaaataaata acaacaagtc
    10081 aacaacctcg acaggatgcc gatgggatga tgaagagata agaaagaata actagtaaag
    10141 ccctaaaacg tcagcgttca gacgcgtttt ttacttaacc ggtaacaaat acgttgtatc
    10201 atagcaacat aaaatgattg ttctaaatca gattaactcc tcacttttgt acaatgtcgc
    10261 ttacaggtat accatgaaat ccgagacgct aactgtccaa caactttttc aagaccgccg
    10321 acaatactgc gttccattct atcaacgtgc ctatgtatgg actcagcgaa accaatggtc
    10381 agctctactg gaggatatcc tagagaaagc acaatcccgg ctctcgggta caaaaccaac
    10441 tccccatttc cttggtgcag tggtactgga acctcaacca aaaaaaggat tattaggtgt
    10501 agattccata catattattg atggtcagca acgattgacc actcttcaat atgttctggc
    10561 atccatccga ttagcattac gtgcgacaga tctttccaac ttagagcctc tcatttcgtc
    10621 ttgcctgaaa aactcaaacg aagacacaat gcgaaataaa gaagtagagc gcttcaaact
    10681 gtggccaact tttcgggatc aaactcattt tattcaaagt tttaatgttg aaaatattga
    10741 cgatatccgg aatgtattct ctgatagttt cacgaagcat ggtacgttgc gtaagcattt
    10801 taatcacccg ccatcactag aagcattatg tttttttact gaagccttta taaagtggat
    10861 taaaatagaa aatcactcac cacgagaaaa tgctgtagca ttaattgagg ctgtcttgac
    10921 ggatctgaaa ctagtaagca tatttctcga ggctgaagat gacgctcaaa taatttttga
    10981 aacattaaat ggcagaggtg cggaacttca tgccacagat cttattcgca attatatttt
    11041 tatgtgcgct gaacatgaaa atattaatgc tgttgaatta tatgagaatg agtggaagat
    11101 ctttgaagat acgta
//
LOCUS       DAZKHC020000122        11074 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZKHC020000122 DAZKHC020000000
VERSION     DAZKHC020000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.122"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..1333)
                     /gene="ulaA"
                     /locus_tag="ACHOSM_RS20010"
                     /old_locus_tag="pgaptmp_004002"
                     /pseudo
     CDS             complement(1..1333)
                     /gene="ulaA"
                     /locus_tag="ACHOSM_RS20010"
                     /old_locus_tag="pgaptmp_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            1688..2752
                     /gene="ulaG"
                     /locus_tag="ACHOSM_RS20015"
                     /old_locus_tag="pgaptmp_004003"
     CDS             1688..2752
                     /gene="ulaG"
                     /locus_tag="ACHOSM_RS20015"
                     /old_locus_tag="pgaptmp_004003"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGU4560397.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            2860..3615
                     /gene="ulaR"
                     /locus_tag="ACHOSM_RS20020"
                     /old_locus_tag="pgaptmp_004004"
     CDS             2860..3615
                     /gene="ulaR"
                     /locus_tag="ACHOSM_RS20020"
                     /old_locus_tag="pgaptmp_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HGU4560398.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3612..4361)
                     /gene="yjfP"
                     /locus_tag="ACHOSM_RS20025"
                     /old_locus_tag="pgaptmp_004005"
     CDS             complement(3612..4361)
                     /gene="yjfP"
                     /locus_tag="ACHOSM_RS20025"
                     /old_locus_tag="pgaptmp_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4560399.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITLEALDAAVTFFRQHL"
     gene            4543..4872
                     /gene="bsmA"
                     /locus_tag="ACHOSM_RS20030"
                     /old_locus_tag="pgaptmp_004006"
     CDS             4543..4872
                     /gene="bsmA"
                     /locus_tag="ACHOSM_RS20030"
                     /old_locus_tag="pgaptmp_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HGU4560400.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            5021..5296
                     /gene="yjfN"
                     /locus_tag="ACHOSM_RS20035"
                     /old_locus_tag="pgaptmp_004007"
     CDS             5021..5296
                     /gene="yjfN"
                     /locus_tag="ACHOSM_RS20035"
                     /old_locus_tag="pgaptmp_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HGU4560401.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(5413..7038)
                     /locus_tag="ACHOSM_RS20040"
                     /old_locus_tag="pgaptmp_004008"
     CDS             complement(5413..7038)
                     /locus_tag="ACHOSM_RS20040"
                     /old_locus_tag="pgaptmp_004008"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HGU4560402.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGVRLILKMGGMTRFDCALGSHAMMRRAFSLAVYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(7122..8285)
                     /locus_tag="ACHOSM_RS20045"
                     /old_locus_tag="pgaptmp_004009"
     CDS             complement(7122..8285)
                     /locus_tag="ACHOSM_RS20045"
                     /old_locus_tag="pgaptmp_004009"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU4560403.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(8288..8926)
                     /locus_tag="ACHOSM_RS20050"
                     /old_locus_tag="pgaptmp_004010"
     CDS             complement(8288..8926)
                     /locus_tag="ACHOSM_RS20050"
                     /old_locus_tag="pgaptmp_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HGU4560404.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFLIVKGWSESSDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            complement(8936..9334)
                     /locus_tag="ACHOSM_RS20055"
                     /old_locus_tag="pgaptmp_004011"
     CDS             complement(8936..9334)
                     /locus_tag="ACHOSM_RS20055"
                     /old_locus_tag="pgaptmp_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HGU4560405.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(9352..10011)
                     /locus_tag="ACHOSM_RS20060"
                     /old_locus_tag="pgaptmp_004012"
     CDS             complement(9352..10011)
                     /locus_tag="ACHOSM_RS20060"
                     /old_locus_tag="pgaptmp_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HGU4560406.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFMLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEIHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(10062..10760)
                     /locus_tag="ACHOSM_RS20065"
                     /old_locus_tag="pgaptmp_004013"
     CDS             complement(10062..10760)
                     /locus_tag="ACHOSM_RS20065"
                     /old_locus_tag="pgaptmp_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HGU4560407.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKVTEAMQRAQQAVTTSIV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(10779..>11074)
                     /locus_tag="ACHOSM_RS20070"
                     /old_locus_tag="pgaptmp_004014"
     CDS             complement(10779..>11074)
                     /locus_tag="ACHOSM_RS20070"
                     /old_locus_tag="pgaptmp_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HGU4560408.1"
                     /translation="GDLQINILFTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMP
                     LSSVGISSVQQEEYYIVFGALSLKSSLEDILLEITSLVDNALDLAEITEEYSH"
BASE COUNT         2928 a         2923 c         2643 g         2580 t
ORIGIN      
        1 gcgcggccag cgaagaacat ataagccagt gcaattacaa tgatgacggc cataaaggcg
       61 ataccgatgg agaagaagcc ctgcatcatc ggcggtgcca gaatcgacca gtccgccatg
      121 cccatccagg cgctcatacc ggtgagtttc accgcccaga cgcagccaaa gatttcaatc
      181 atccccatca ccagacatat cttcagcgcc gcacgccagc cgccgaagtg gttagcgaac
      241 acgccgatgg tggcgttgga gaagaacatc gggataaagc cgggaataat caggatcgag
      301 gaaccgcagg cgaccaggat gccaaccgca atcagctgac cgatggtgcc ccacataaag
      361 ccccaaacca cggcgttcgg cgcgaagcta tagatagccg cacagtcaat cgccagaacc
      421 gcaccaggga tcaggcgctg ggagataccg ttaaatgctt cagagagttc cgccacaaac
      481 atgcgcacgc cctgagtgat gatgaagatc gccaccgcga aggagaaacc agtttgcagg
      541 atgtacaccg tccagttcac tttgcctgcc atcgcctgca cagtgtcgat accgaaggag
      601 agcagaatgg caccaaagaa gatggtcatc acaatcgccg tggagacgat gttgtcgtgg
      661 aaaatattca gccagcctgg cagtttgagg tcttcaacgc tttcctcttt tttgccgagg
      721 aacggcgcaa ctttataggc aatccatgat gcaaactgtt gctggtgacc gatggagaaa
      781 ccgcagccgt ccgtcacttc ctgagtcggc ttgtacatca tgttggaggt aatgccccag
      841 tagagcgaaa ccagaatcgc cgtgcagata atggttgtcc acatggagta gccgaaaata
      901 aacaacgtaa cggcaatcag ccccgcctgc tggaacatga tatgaccagt cagcatgatg
      961 gtacgaatac cggtaatgcg acgcagcagc acgtaacaga tgttcagcgc cagcgctaac
     1021 aacaccgcgt aacctaccca gctataggca tcgcccatgc ggtcgatggt tgccatcatc
     1081 gacgcgtaag tatcggaaat tgcgccgtta atgccgtaga cttcggacat tttcgccacc
     1141 accggtttga aagtgctggt gaggatgccg gaccctgcct gcaacaacat gaaaccaatt
     1201 atggttttaa tcgtgccttt acacttttgc gcagtaggat gtagcccaga caggtcacaa
     1261 tacccagcaa caacggggca ttggtcatga cctggttaaa aaacacggta aagatgttgt
     1321 agaggatctc cataacgatc tcctgaagaa gaggtaaccg ggtattccgc acacccggaa
     1381 tgttgtgcac tcactataat tttcaaaagt aatcacaaca agattattta tgattaaatg
     1441 tgacgcgacc cgcaaataat tccacacgga tagagtggga ttcacacaac gaaacaatta
     1501 tttatttaaa ttcatttagt taatttaact acacattcca ttaaatcttt ccacactcaa
     1561 aatcaacaac aacttccctg gcaaaaagtc aaaaaatggt cattgccttc ctgaaaaaat
     1621 agtgtcagga ttaatcaaaa ccaatcacat attgattcga attgaactat catgaaggta
     1681 aatggcgatg agtaaagtga aaagtatcac ccgagaatcc tggatcctga gcactttccc
     1741 ggagtggggt agctggctga atgaagaaat tgaacaagag caggtcgccc ctggcacatt
     1801 tgcgatgtgg tggctaggct gcaccgggat ctggttgaaa tcagaaggtg gcaccaacgt
     1861 ttgcgttgat ttctggtgcg gcactggcaa acaaagtcac ggtaacccgt taatgaaaca
     1921 gggtcaccag atgcagcgca tggctggcgt gaaaaaacta cagccaaacc tacgcaccac
     1981 cccgtttgtt cttgatccgt ttgcgattcg ccagatcgac gcggtactgg cgactcacga
     2041 tcacaacgat catatcgacg ttaacgtcgc tgctgccgtg atgcagaact gtgctgatga
     2101 cgtaccattc attggaccga aaacctgcgt tgatttgtgg attggctggg gcgtaccgaa
     2161 agagcgttgc atcgtggtca aaccgggcga tgtggtgaaa gtgaaagaca ttgaaattca
     2221 tgcgcttgat gctttcgacc gtactgcact gatcaccctg cctgccgatc aaaaagcggc
     2281 tggcgtactg ccggacggca tggacgatcg cgcggtgaac tatctgttca aaacgcctgg
     2341 cggctccctg tatcacagcg gcgactccca ctactctaac tattatgcga agcacggtaa
     2401 cgaacatcag atcgacgtgg ctttaggttc atacggcgaa aacccgcgcg gtatcaccga
     2461 caaaatgacc agcgccgata tgctgcgtat gggtgaagcc ctgaatgcga aagtggttat
     2521 cccgttccac cacgatatct ggtcaaactt ccaggccgat ccgcaagaga tccgcgtgct
     2581 gtgggagatg aaaaaagatc gtctgaagta tggcttcaag ccgtttatct ggcaggttgg
     2641 cggcaaattt acctggccac tggataaaga caacttcgag taccactatc cgcgcggttt
     2701 cgatgattgc ttcactattg aaccggatct gccgttcaag tcattcctgt aatctgtcgt
     2761 ttataccgga tagcgtggag aagttcgcgc tatccggcgt agtatttcct ggctatttca
     2821 aatatcatct aaaaaaatca aattttatcg gaatagctca tgactgaagc acaaagacat
     2881 caaatcctcc tggaaatgct cgcacaattg ggctttgtga ccgttgagaa agtcgttgag
     2941 cgtctgggaa tttcgcctgc cactgcgcga cgcgatatca ataaacttga cgaaagcggc
     3001 aaactgaaaa aggtgcgcaa tggcgcagaa gctattaccc aacagcgccc gcgctggacg
     3061 ccgatgaatc tgcatcaggc gcagaatcac gatgaaaaag tacgtattgc taaagcggcc
     3121 tcgcagctgg ttaatccggg cgaaagcgta gtcatcaact gcggctccac cgcgtttctg
     3181 cttgggcggg aaatgtgtgg caagccagtg caaatcatca ctaattatct accgctggca
     3241 aattacctga tcgatcaaga acatgacagc gtgatcatta tgggtggaca gtacaacaaa
     3301 agtcagtcca tcactttaag cccgcagggc agcgaaaaca gtctctatgc cgggcactgg
     3361 atgtttacca gcggaaaagg gctgaccgca gaagggttgt ataaaaccga tatgctgaca
     3421 gcaatggcag agcagaagat gctgagcgtg gtagggaaac tggtggtact ggttgatagc
     3481 agtaagattg gcgaacgcgc gggaatgctt tttagccgtg ccgatcaaat cgatatgctt
     3541 atcaccggca aaaatgctaa cccggaaatc ctgcaacaac tggaagcgca aggggtcagc
     3601 attctgcgtg tttaaagatg ctggcggaaa aatgtcacgg cagcatctaa cgcctcaagc
     3661 gtaatgcggt gacgcacgcc tggctgccat gaacaggtta gcagtttatc ccgtcccgtc
     3721 tcgcttaagg cctgctgcaa acgtagtgat tcgtcggcag gcacaacgtc atcgtcgagg
     3781 ccatgccaca gcagtagagg tctgtcccca agttgttcca ggtggtttgt cgcttcccac
     3841 tctgccagcg gcgcgataat gttattgaat tcattctgct gtgctgccgt ttcaggtatc
     3901 agcggtggaa acagtgaacg ggcgagcgat gtaaaatagc ccgatcccat catgctggcg
     3961 gtacatttca ccgtggggtg gcgagcggta attcccagtg ccgtcatcgc gcccatcgac
     4021 gcgccaccga ctgccagacg gtcatcaagc agccagtttt cttcggctat tgccgcacgt
     4081 aaagtagtga attcctgcat actttgtagc aagatttgcc agaattgatt taaccgccgc
     4141 gcttcgtcgc cactaaaacg gctaccgtga tcgggcgcat ccggcatgat cacccgtaaa
     4201 ccagcctgcg ccagcgcaac ggcaaaatag ctatacacca ggctggatga agtaaagccg
     4261 tgataaaaaa ttacgcacgg taacggggta tctttttgcc cgacaggata agcatgaaga
     4321 acgggaatat ctgccagctc gcgtgattct atttcaatca tctgtctctc ctttttgtct
     4381 ggtatgacat gaagaaatcc ttagcacaag tttctgctgg cggtattagt tcataatgtt
     4441 gagatgtggg ttacgctttc gtcacgtttt cattcgaaaa tcgcgtacca ggtaacaatt
     4501 cgggaacatt cccccaaagt taaattaata aggcactaca ctatggttag caggaaacgt
     4561 aatagcgtta tttaccggtt tgccagttta ttattggtgt tgatgttaag tgcctgtagc
     4621 gcactgcaag gtacgccaca gccagcaccg ccagtaacgg accatccgca agagattcgc
     4681 cgcgaccaga cgcaagggtt acagcgaata ggtagcgtaa gcacgatggt tcggggttct
     4741 ccggatgacg cattagcaga aatcagagca aaagctgtcg ctgcaaaagc tgattattac
     4801 gttgtcgtca tggtggacga aaccatcgtg acaggacagt ggtattcaca agccattttg
     4861 taccgtaaat aaatcagaca acttacatag tcagctttac attgctttgc gtccctatgc
     4921 gtttacctgt gcacaataaa ttaccggctg gaagcctggg cgtttattac cgcgatggaa
     4981 tgccctgcaa catgtgggga aacgaaaaat ggagctgacg atgaaacaat tacttgcctc
     5041 accctcgctg caattagtga cttatcctgc gagcgccacg gcacagtctg cagaattcgc
     5101 cagtgctgac tgcgtgacgg gcctgaatga aataggtcag atctcagtta gcaatatttc
     5161 aggggatccg caggacgtgg aacgtatcgt ggcgttaaag gccgatgaac aaggtgcgtc
     5221 atggtaccgc attattacca tgtacgaaga ccagcagcct gataactggc gcgtacaggc
     5281 gatactgtat gcatgagtgt catctacagc aaatgaacaa tgccggatgc ggcgcgagcg
     5341 ccttgtccgg cctacaattt tcacatattt cattgagtta atcgaaacca gcgtcgcatc
     5401 agtcgtatac gcttacacac acactccccc cgtcgcccgc agcaataaat cattctggat
     5461 ctgctctgac aaccgtacgc cgccgcgcgt atctaacatc acctgacacc acgcctgcgc
     5521 cattggcgga gaggcatatt tcaacatttg cgcaccgcag cccagcagga atagctgatg
     5581 agtaatctct cgccccaatt cttcagctgg cttgcgcagc tgctgctgta aacgacgaac
     5641 cgcgcgatca aaatagcgat cctgcccttt cacttccaca aacgcttccg acaataagtc
     5701 gtatacgccc gcttgcttat tgagaacgcg cagcacatcc agacacataa tattgccgga
     5761 accttcccaa atactgttta ccggcatctc ccggtaaagc cgcggcaact cgctctcctc
     5821 gcaataacca atgccgccca gcacctccat cgcttcggcc acaaacggca tacctctttt
     5881 gcagatcaca aattttgccg caggtgtaaa taaacgcgcc cacagagctt ctttggcatc
     5941 ggcgcgccgg tcccacgctc gcgcaagacg aaacagcaac gccgtttgcc cttcaagctg
     6001 aagcgccatg cgacttaaga catgacgcat aaggggctgt tggatcaatg gattaccaaa
     6061 aacatggcgt tgatgtgcat gataaaccgc cagcgaaaat gcacggcgca tcatggcatg
     6121 gctacccagg gcgcaatcaa aacgcgtcat cccccccatt ttcaggatca gacgaactcc
     6181 ttccccttcc tgccccaaca accagccaat ggcatcctga aactccactt cgcagctggc
     6241 gttagagcga ttacccagct tatctttcag ccgctcgagg cgaatcgcgt tgcgttgccc
     6301 gtcaggcaaa aagcgcggca caaaaaagca ggacaaaccg cccgtagtct gcgccagcac
     6361 cagatgcgca tcgctttgcg gcaccgagaa aaaccattta tgccccacca gccgataaga
     6421 gccatcttcc aggcgctctg cgcgggtggt gttgctcatg acatcggaac cgccctgctt
     6481 ttccgtcatt cccatgccaa tcaacaaacc gcgtttttgc ccacctggca ataagtgaga
     6541 atcgtagcga tcgctcagca gcggcgtggt ccagtcctga aacggcgcgg gtaacatctg
     6601 taacagcaat ggcgtggcgg caaaggtcat ggttatcgga cataacgacc ctgcctcaac
     6661 ctgtgcgtgc aacataaaac gcgccgcgcg cgccacaaat gcgccggagc gagcgtcttc
     6721 ttcccaggca agattgtgca cccgattggt acatagcgcc tgcatcagca ggtgccaggc
     6781 ggggtgaaaa cgcacatcgt ccagacggcg tccttgcgca tcgtagcgca ataattcagg
     6841 cggattcaca ttcgccagcc gccccagttc aagggattca gccgttccta actgctgacc
     6901 aatactggct agaaaatcgc tatcccagcc agcaccttca cgcgttaccg cttcgcagag
     6961 cgcgccatca gacaggtata agttgctgtt atttaatggt ataggttgat taaaaacggt
     7021 gtgagtttgc cagtgcactg tgtctccctc catcaatggc agtcaccatt agtatggtca
     7081 ctgccatgga ttcatgacag aaagagattg ctaaaacatt cttatccagc aatgtaatgc
     7141 ggaacgaaac gtgaggtgtc tttggtgatc agtgtgttat cttcacggat ccccattccg
     7201 cacgcttcat catcgacaat ccagctaccg atgagtgtgt agctatcgcc aaatcgcggc
     7261 agaggttgaa acgcctggta gatcatcggt tcatcggcgt aatcaccatc agcgtggtca
     7321 acgacgttat tctgaccgtc gaaaatggtg acgtttccgc cttcgcgcga gtagatcggt
     7381 ttacgcacat agctttcgcc agcggcaatc tgcgattttt caccctcaaa ccaggatgca
     7441 agaaggttag gatgaccagg gaagaagcgc caaagcagcg gcattagccc tttattactc
     7501 aagatacttt tccataacgg ctccacccat tgctcgcgac gcttgcgcag cagcggaccg
     7561 ttatcgtcac gcatcatcca ctccagcgga tacagcttaa atgcacgctg gatgacatta
     7621 tcatcaagat cggtcagtac gccgccgacg cccaaaccga gatcttcaat gtagataaac
     7681 cgcgactcct gccctgcctg ctgggcgcag tcctgcaagt acagcacggt agtcctgtct
     7741 tcatcggtgt cctgacagca gcagaaataa aacggctccc ggctataaag ctcgctaaag
     7801 cgtgaaatca ggcgttcctg aatggcattg tactgatcgg catcacgcgg aataatgccg
     7861 ctgcgccggg catcctccag ccacaaccac tggaaataag ccgactcata caacgaggtc
     7921 ggcgtatcgg cgttgtactc cagcagcttc accggcgcat tgccacacca ggcaaaatcc
     7981 atccggccat acagcgaagg atcgcgtgcg tgccagcttt cggcgatcac atcccagtac
     8041 aacggcggga tcgccagttg cgtcaggatc tcttcgtctt taaccgcgcg atccaccacc
     8101 tcaaggcaca tctgatgcag ttccgcagtc ggtttttcga tctgctcttc aatctggcgc
     8161 agagtaaaac ggtaagcccg actctcatcc caatagattt cattgtcgat gatatgaaag
     8221 tcgaaaccgt tgtcagcggc gatctggtcc agatcccgcc gcacaggaac gttgtgtctc
     8281 agcatgatta gcctccccaa tgcccacggg cgctggaaga acgaccatag ccgccgcgcg
     8341 aaacggtcga cgcttttctg gtggtaaagc cgcctgaaga gtaagactct tttttgccag
     8401 aaccggagcg ccaggagtaa tcaccagaag tgttgcgcca gacggggcgc gaagcaaaag
     8461 aggaaccgcc gctgttataa acaaactgct catcgcgatc tttacgaata acccggctta
     8521 acaaaaatcc agaaacgacc gggatccaac tctgttcaac gttgtcgtag taacaatttt
     8581 catacttaga ctgacaattc tgctgagtca tattcttcgg aacatcggca taaaatgctg
     8641 ctttggcgtt gttccagcca cgcgcgcaaa tgtcggcatt attaccgtca tcaatacaat
     8701 cctgcacagt cgagtaaaac gttccgtcgc cgtcgttatc gacatcgcta ctttcactcc
     8761 atcctttgac tattaaaaaa gcggcaccgc ccattagcgc caacgttaag tatttttgtg
     8821 cgtaacgatt acgacgcggt tcaaaaggat tattcggtct accaatgcga ctgatcgctc
     8881 cgtgaccaat tttactgtta tttcttgatt tgcgttttct ggccatgatc gtccattacc
     8941 atgtcataca agctgcgtta aaaataccgc cagccagcgc ggcagttccc ataaacattc
     9001 ctgctgcggt attgtgatta ataatttttt cgcttaatgc gggcatataa agtctgacac
     9061 cagcaaaaac gagtaactga atcaccagtg caatcccgcc ccaggcgaaa tagtctggaa
     9121 tactcaccgc attgattgcc gcactggata aggggatcac gtaacccaac aatgtaccgc
     9181 tgaatgccag tgacgccgcg gtattattgt ttttgattaa ctgccattcg ttgtgcggtg
     9241 taattttgga gtagataaac agaaaaataa tcaccatagc cacgccaata aaaaaatagg
     9301 cactaaaggc aagaagtgaa tccagtatat gcattgtaaa cgtccttgta attaaccaat
     9361 aatatgaagt gatgtcagcg ggatatcgac acccaacgcg cgcgaaaata accactctgg
     9421 ctcgccgaga tcgttaaaag attcctcacc atttagcagc aaatattcgt aagtatcttc
     9481 ggtcacttgc cgctggtagc ccatggtaaa attatggatt tcccatttgg catggttttg
     9541 attttctact ttttccagca tgtagacggg ttcgatattt cccggttctt cgctgttaaa
     9601 aaatcgctgc cagcgttttt cctgccagtt taaggtcatt gcccccatcg ttttggcgtt
     9661 gatcgcctcg cgccagtgac tctctttact gataccgtag ctctcttcat agacaaagag
     9721 cttgatatca tcaatgtcat caatatcttc gccgccggtg gtattgattt gcagaaactc
     9781 atcgcctgaa gtgtagtagc ggaaaatctg actaccgccg cccagatcga tgtggctgac
     9841 agcagctacc gtaaattcct cacccggcag cgcaatcagc aattcatctt ccagcaaacg
     9901 aaacgctaac gtgtcgagca taaagccact attgagatga agtcccagcg gaccgcgagc
     9961 gatagcaggc ttattatcct tgccaaacag acgctggaaa aaaccagaca tattttatcc
    10021 ttaaaatagt tgccagcctt ttagcgaggc tggcaaaaaa attactcgcc ctgttggcgt
    10081 tgcagtcttg ctaatacatc ctgggcgcta cttttattgc taccgccaat tccggcttcc
    10141 gccagctttt catcaagatc gcgaccgtct gcgacttttt ccaactgtgc agcagcatcc
    10201 aggcgagcct gacgttcggc ctgacgcgtt tgcaggcgtt ttaaggattc tgccgctgtc
    10261 gaaacgctgg aagatgcgcc aacgatagag gttgttacag cctgttgtgc acgctgcatg
    10321 gcttcagtga ctttaacgac ttccatttgc tgctcaaact gagcgatacg ctgcgctgtc
    10381 gctgtaactg ctttttccac gccatcacgg gagacttcca gattcgacaa cacttgctct
    10441 tcagcggtaa tgagattctc aaggcgcgcg atttcttcag caacttcgtt aatcaacgac
    10501 ggattaacgt tcttgcttaa cgcttccagc gcacgggctt ccagactggc tttgcgctca
    10561 cgtaaatctt tcagcttatc gtgacttaat ttcacccgcg ccagcagatc aacgcgagat
    10621 tttccggctt tatcgagttc agctttagcg tcacgaatat gctgttccag catgcgcacg
    10681 ccctgggttt cttcaatgga ttcttccgcc tgggagataa acgatttccc cagcgtaaat
    10741 aaacttttta aaattcccat aaatacccgt ccttgtaatt agtgtgaata ttcttctgta
    10801 atttcagcca gatccaatgc gttatctacc agcgaggtta tctcaagcag gatatcttca
    10861 agagaagatt taagcgataa cgccccgaaa acaatgtaat actcttcctg ttgcacgctg
    10921 gagattccta ccgatgacaa cggcatcatc ttctgatttc ttaataagaa ggtattaaat
    10981 tcatctggat tgctgatact acttaccgga caaataaagg tttcgataat catttggcgg
    11041 gaagtaaaaa gaatattgat ttgcaaatcg ccat
//
LOCUS       DAZKHC020000123        10898 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZKHC020000123 DAZKHC020000000
VERSION     DAZKHC020000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.123"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            204..5623
                     /locus_tag="ACHOSM_RS20075"
                     /old_locus_tag="pgaptmp_004015"
                     /pseudo
     CDS             204..5623
                     /locus_tag="ACHOSM_RS20075"
                     /old_locus_tag="pgaptmp_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            5832..6098
                     /gene="hipB"
                     /locus_tag="ACHOSM_RS20080"
                     /old_locus_tag="pgaptmp_004016"
     CDS             5832..6098
                     /gene="hipB"
                     /locus_tag="ACHOSM_RS20080"
                     /old_locus_tag="pgaptmp_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HGU4560409.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            6098..7420
                     /gene="hipA"
                     /locus_tag="ACHOSM_RS20085"
                     /old_locus_tag="pgaptmp_004017"
     CDS             6098..7420
                     /gene="hipA"
                     /locus_tag="ACHOSM_RS20085"
                     /old_locus_tag="pgaptmp_004017"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HGU4560410.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKEITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRCWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7732..7940
                     /locus_tag="ACHOSM_RS20090"
                     /old_locus_tag="pgaptmp_004018"
                     /pseudo
     CDS             7732..7940
                     /locus_tag="ACHOSM_RS20090"
                     /old_locus_tag="pgaptmp_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8373..8936
                     /gene="fimA"
                     /locus_tag="ACHOSM_RS20095"
                     /old_locus_tag="pgaptmp_004019"
     CDS             8373..8936
                     /gene="fimA"
                     /locus_tag="ACHOSM_RS20095"
                     /old_locus_tag="pgaptmp_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4560411.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            9297..10007
                     /locus_tag="ACHOSM_RS20100"
                     /old_locus_tag="pgaptmp_004020"
     CDS             9297..10007
                     /locus_tag="ACHOSM_RS20100"
                     /old_locus_tag="pgaptmp_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4560412.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            10049..>10898
                     /locus_tag="ACHOSM_RS20105"
                     /old_locus_tag="pgaptmp_004021"
     CDS             10049..>10898
                     /locus_tag="ACHOSM_RS20105"
                     /old_locus_tag="pgaptmp_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HGU4560413.1"
                     /translation="MTAIRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNF"
BASE COUNT         3024 a         2303 c         2798 g         2773 t
ORIGIN      
        1 ctcatggttc atttttaaat aaaataagaa ctatgcaatt tttaatctat caattcgaaa
       61 gtattagcaa attagccaaa ttgacacctt cccacctcca accaacactc aatacagacc
      121 tctgacaatt taataagttt catttttaat gtctgacgac tcatctcttt cgtggctgta
      181 ttgtttgtat atggagcgtt tatatgaata gaatctatcg cgtgatatgg aattgcaccc
      241 tacatgtatt tcaggcctgc tcggaattaa ctcgcagggt aggtaaaaca tcaacggtta
      301 atttgcgtaa atcctctgga ctgacaacga aattcagtag attgacgctg ggtgttttgc
      361 tggcactaag cggttcagcg tctggtgcga gtctggaagt tgataatggt cagattacca
      421 atattgatac tgatgttgct tatgatgcct acctggttgg ctggtatggc actggagttc
      481 ttaatatttt ggctgacggt aatgcctcct taaccactat tactaccagc gtcattggcg
      541 ctaatgagga ttcggagggt accgttaatg ttttgggtgg cacctggcga ttgtatgata
      601 gcggaaataa tgcaaggcct ttaaatgtgg gtcaatccgg aacggggacg ctgaatatta
      661 aacagaaggg tcacgtcgat ggaggctatt taagattagg ttcttcgaca ggaggcgtcg
      721 ggacggtcaa tgttgaggga gaggactctg ttctgacgac cgaattattc gaaataggga
      781 gttacggtac gggctcatta aatattacgg ataagggtta cgtcacgagt tcaatcgtcg
      841 ccattttagg ctatcaggcg ggcagtaatg gccaggttgt cgttgaaaag ggtggcgagt
      901 ggctaataaa aaataacgat tcctcaattg aatttcaaat tggtaatcaa gggatggggg
      961 aagcgactat tcgcgagggg gggctggtta cggctgaaaa tacgattatc ggtggcaatg
     1021 ccaccggtgt cggaaccctg aatgtgcagg atcaagactc tgtcatcacg gtacgcagac
     1081 tctataatgg atatttcggt aatggcgcag tcaatatttc caataatgga ctaattaata
     1141 acaaagaata ttcattggtg ggcgttaaag acggttccca cggtgtcgtc aacgtgaccg
     1201 ataaagggca ttggagtttc ctcggaacag gcgaagcttt ccgctatatc tatatcggtg
     1261 atgctggtga cggtgaactt aatgtctcgc gtgaaggtaa agtagattcg ggaattatca
     1321 ctgcggggat gaaagaaaca ggcacaggca acattactgt taaggataag aactccgtta
     1381 tcactaatct cggaactaat cttggttatg acggccacgg cgaaatgaat atcagtaatg
     1441 aggggcttgt tgtcagcaac ggaggaagct cactcggtta tggagaaaac ggcgtcggga
     1501 atgtcagcat caccacgggg gggatgtggg aggtcaataa gaatgtctat accaccattg
     1561 gtgttgcggg cgtcggaaac ctcaatatta gcgatggcgg taagtttgta tcgcaaaata
     1621 ttactttttt gggcgataaa gcaagcggta tcggcacact gaacctgatg gatgggacat
     1681 catcgttcga tactgtgggt atcaatgtcg gtaattttgg tagcggtatc gtaaatgtca
     1741 gtaatggtgc cacccttaat tcaacgggct atggatttat cggaggaaat gcctccggta
     1801 aggggatagt taatatttca acggatagtc tctggaattt aaagacatca tctactaacg
     1861 cccaattgct acaggtcggt gtattaggca caggtgaact gaatattacc accggaggta
     1921 tagttaaagc gcgtgataca cagatagctc tcaatgacaa aagtaagggc gacgtgaggg
     1981 tggatgggca gaactctctt cttgaaacat tcaatatgta cgtagggaca tctggtacgg
     2041 gtacgttaac cctgacgaat agcggcacgc tgaatgtcga aggtggagaa gtttacttag
     2101 gtgtttttga acctgctgta ggaacgctaa acattggtgc agctcacggt gaggcggcgg
     2161 cagatgccgg atttatcacc aacgcgacga aagtagagtt tggttctggc gaaggtgttt
     2221 ttgtctttaa tcatactaat aacagtgatg ccggctacca ggtcgatatg ctgattacag
     2281 gtgacgataa agacggaaaa gtgatccatg atgcaggcca tacggtgttc aatgcaggga
     2341 atacttatag cggtaaaact ctggtcaatg acggcctcct gaccattgcg tctcatacgg
     2401 cagatggggt aacgggcatg ggttcgagtg aagtcaccat tgcaagcccc ggtacgctcg
     2461 acattctcgc atcaacgaac agtgcaggag attacacgct gaccaatgcg ctcaaaggcg
     2521 atggtttgat gcgagtgcag ctgtcatcct ccgacaagat gtttggcttt acccatgcaa
     2581 cagggactga attcgccggt gttgcccaac tgaaagacag taccttcact ctggaacgcg
     2641 acaacaccgc tgcgcttact cacgcgatgt tgcagtctga cagtgaaaat accacatcgg
     2701 taaaagtggg agagcaatcc attggtggac tggccatgaa tggcggtacg ctcattttcg
     2761 atacggatat tcctgctgcg acgcttgcag agggatatat cagcgtcgat acgctggttg
     2821 tcggcgcggg tgactacacc tggaaaggcc gtaactatca ggtaaacggg acgggcgacg
     2881 tgcttatcga cgtgcctaaa ccgtggaatg atcccatggc gaataaccct ctgacgacgc
     2941 tcaatttgct ggaacacgat gatagccatg tcggcgttca actggtgaag gcgcaaacgg
     3001 ttattgggtc gggtggctca ttaacgttac gtgatttaca gggcgacgag gtggaagcgg
     3061 acaaaacgtt acacattgcg caaaacggaa cggtggtcgc cgagggtgat tatggattcc
     3121 gcctcacgac cgcaccaggt gatggtttgt acgttaacta tgggctgaaa gcgctgaaca
     3181 tccatggagg gcaaaagctg acgttagccg aacatggcgg agcctatggc gcaacggccg
     3241 atatgtcggc aaaaatcggt ggtgaagggg atctggcaat caatacggtg cgacaggttt
     3301 cgctttccaa cggtcagaac gactatcagg gggcaaccta cgttcagatg gggacattac
     3361 gtaccgatgc ggatggtgca cttggcaaca cccgggaact gaacatcagc aacgcggcca
     3421 tcgtcgatct taatggatcg acgcagacgg tagagacatt caccgggcag atgggttcga
     3481 ctgttttgtt caaagagggg gcgctgacgg taaataaagg tgggatcagt cagggtgaac
     3541 tgacaggtgg cggaaacctg aatgttacag ggggaacgct ggctatcgag gggcttaatg
     3601 cacgctacaa tgcgttaacc agcattagcc caaatgcgga agtcagcctc gataatacgc
     3661 aggggttagg cagaggaaat attgccaata acggtctgtt aacgctaaaa aacgtgactg
     3721 gcgaactgcg caatagcata agcgggaagg gtatcgtgag cgcaaccgcc aggacagatg
     3781 tagagctgga tggcgataat agccgctttg tggggcaatt caacattgat acaggcagcg
     3841 cgctcagcgt caacgagcag aaaaacctgg gtgatgcttc cgttatcaat aatggcctgc
     3901 tcaccatctc cactgagcgt agctgggcga tgacgcacag tatcagcggt agcggtgatg
     3961 tgacaaaact gggtaccggg atcctgactc ttaacaacga ttccgcggcg tatcagggta
     4021 cgacggatat cgtggggggg gaattgcttt cggttccgac tctgccatta atatggcaag
     4081 tcaacacatt aatatccata acagcggtgt gatgtcggga aatgtcacca ctgcaggtga
     4141 tatgaacgtt atgcctgggg gggcactgcg tgtcgctaaa accactatcg gcggcaacct
     4201 ggagaatggc ggcacggttc aaatgaatag cgaagggggg aaaccgggga atgtactgac
     4261 cgttaacggc aactataccg gaaacaatgg cctgatgacg ttcaacgcga cgctgggcgg
     4321 cgataattcg cccaccgata agatgaacgt gaaaggcgat acccaaggga acactcgcgt
     4381 tcgggttgat aacattggcg gcgtcggtgc acaaacggtc aacggtattg aactcattga
     4441 ggttggcggt aattctgcag gtaacttcgc gctgaccacc ggaactgtcg aagctggggc
     4501 ttacgtctac acgctggcta aagggaaggg gaatgacgag aaaaactggt atctgaccag
     4561 taaatgggac ggcgtaacgc cagcggatac acccgatccc atcaataatc cccctgttgt
     4621 ggatccggaa ggcccatcag tttatcgccc ggaggccgga agctatatca gcaacattgc
     4681 cgcagccaac tcgctgttta gccatcgttt acacgaccgt ctgggcgagc cgcagtatac
     4741 agattcactg tattctcagg ggtcggcaag cagtatgtgg atgcgtcatg tcggagggca
     4801 cgaacgttca agggccggtg acggtcagct aaatactcag gctaaccgct atgtattgca
     4861 gctaggcggc gatttggcgc agtggagtag caacgcgcag gatcgctggc atcttggcgt
     4921 gatggcaggt tacgctaatc agcacagtaa tactcagagt aatcgtgtgg gttataaatc
     4981 ggatgggcgc atcagcggtt acagcgctgg gctgtacgcg acctggtatc agaacgatgc
     5041 gaataagacc ggcgcttatg ttgacagctg ggcgctgtat aactggtttg ataacagcgt
     5101 cagttccgat aaccgttctg ctgacgacta tgattctcgc ggtgtgacgg cctctgttga
     5161 gggtgggtat acctttgaag cgggaacatt tagcggcagc gaagggacgc tgaatacctg
     5221 gtacgtccag ccacaggcgc aaatcacctg gatgggtgtg aaagattccg accatacccg
     5281 gaaagacgga acgcgcattg aaacggaagg cgacggaaat gtgcaaacgc gacttggggt
     5341 gaaaacctac ctgaacagcc atcaccagcg tgacgatggt aaacagcgtg agttccagcc
     5401 ttacattgaa gcgaattgga tcaacaacag caaagtctac gtcgtgaaga tgaatggtca
     5461 aaccgtaggc cgtgaaggtg cgcgtaatct cggtgaagta cgtaccgggg ttgaggcgaa
     5521 agtaaataac aaccttagcc tgtgggggaa tgtcggtgtg caactaggtg ataaaggcta
     5581 tagcgatact cagggcatgc tgggagtgaa atatagctgg taaaccgtat aagccgcatg
     5641 tcgagatggc atgcggctta atattgccga cttcaaaggc tttaaagcct cccttttatc
     5701 cgcgatcgcg gatatcgcag cgtttatccc gtacagcgga taagatgtgt ttccagattg
     5761 actttatcct cactaaagga taaaactgat aatatcccct taagcggata aatttgctgt
     5821 ggacgtatga catgatgagc tttcagaaga tctatagccc aacgcaatta gcgaatgcaa
     5881 tgaaactggt tcgccagcaa aatggctgga cgcagagcga gctggcgaaa aaaattggca
     5941 ttaagcaggc gacaatttcc aatttcgaaa acaaccctga caataccacg ctcacgacat
     6001 tttttaagat tttacagtcg cttgaactct caatgacact atgcgacacg aaaaatgcct
     6061 cgccagaatc aacagaacag caagatctgg agtggtaatg cctaaacttg tcacttggat
     6121 gaacaaccag cgggtaggcg agttaacgaa gttagccaac ggcgcgcaca cctttaagta
     6181 tgcaccggag tggttagcca gccgttatgc cagaccgttg tcactttcgc tgccattgca
     6241 gagggggaat atcacctcag atgccgtatt taacttcttc gataacctgt tacccgatag
     6301 cccgattgta cgtgaccgga tcgttaaacg ttatcatgct aaatccagac aaccgtttga
     6361 tttattgtca gaaatagggc gagacagcgt tggtgccgta acgttattac ccgaaaatga
     6421 aaccataacg cgcccgataa tggcatggga aaagcttact gaagccaggc ttgaagaagt
     6481 attaacggct tataaagcag atatccctct gggcatgatt agagaagaaa atgactttcg
     6541 catctcggtt gctggcgcgc aggagaagac agcgctgctt agaataggca atgactggtg
     6601 cattccgaaa gaaataacgc cgacgacgca catcattaaa ttaccgattg gcgaaatcag
     6661 gcagcccaat gcgacgctcg atctcagcca aagcgttgat aatgagtatt actgtctgct
     6721 gctggcgaaa gaacttgggt tgaatgttcc ggacgcagaa atcattaaag cgggaagggt
     6781 gcgcgcgtta gcggtcgaac gttttgacag gtgttggaat actgagcgaa cggttttact
     6841 tcgcttgcca caggaggata tgtgtcagac attcggttta ccttcatctg tgaaatatga
     6901 atcagatgga ggcccaggca tcgcgcggat catggcgttt ttgatggggt ccagcgaggc
     6961 gcttcgcgat cgttatgatt ttatgaaatt ccaggtcttc cagtggttga ttggcgcaac
     7021 ggatggccat gcaaaaaact tctccgtatt tattcaggct ggcggcagtt atcgactcac
     7081 gccattttac gatatcattt cagcatttcc ggtccttggc ggtacgggaa tacacatcag
     7141 cgatctcaaa ctggcaatgg ggcttaacgc atccaaaggc aaaaaaacgg caatcgataa
     7201 aatttatccg cgacattttt tggcgacagc aaaggtgctg agattcccgg aagtgcagat
     7261 gcatgaaatc ctaagtgact ttgccagaat gattccggca gcactggata acgtgaagac
     7321 ttcattaccg acagattttc cggagaacgt ggtgacggca gttgaaacca atgtgttgag
     7381 gttgcatgga cggttaagcc gagaatacgg tagtaagtga gatatggggt ttggtcgttg
     7441 ttaatgagca cgacaatcat gaccgccaac gactggattc ttccttggtt tatactggtg
     7501 cgcataatgt atattatgtt aaatcatgtc tgagtgatgc gaagtgcttt tctggtagtc
     7561 gttattcgtt cagggttcaa gaacttaagc ctctcctctt tcataattgg cctgtgcatg
     7621 gagaaatagc ctggttattc cctgccccat gctaagagcg cagtgtctgt cgcatagaca
     7681 ctgcatacga aaacaccaga gggtgctgtt acttatttaa ggacggatca agcgggaatg
     7741 agtccactcc ggtatcagaa atggctacgt ctcaatgaag ccaggcgacc gatgctgaat
     7801 gaacattacg atgtcaccac agcagcctat gctgtcggtt acgaaagcta tcccatttca
     7861 gtcgggaata ttcgcggatg tttggagagt cacccaagag agatattacc aggttaagaa
     7921 agtctgtcgg tcagctttaa taaaccctgc acttatctgt ttagagcaat tcggtgttag
     7981 tttcagcaag caaacattaa ccatagctaa tgatttatag ccatattaac cattggtagc
     8041 ccttgacctt tcctgacact tcccttgttc gcaataatat tctgtttagt cacggtttat
     8101 aaataacatg cataatgaaa tcgtcatgtt atttagcgac ataaaatgtt tttgcaaata
     8161 ctctcttcaa caagtgcagc aataaacttg ttgtgattag gtgtccttgt gatttaaaga
     8221 tattatcgtt cttcgtgaaa tgtaataacc ctggaaacta tattctcagg gcaacaacca
     8281 atcaagaatg attcttgttt ttaagcaata acagaatgac ttaattgtta actctcttta
     8341 ttcagctact taaatataaa ttttggagaa ttatgaaact caaacatgtt ggtatgattg
     8401 tcgtttctgt gttggcgatg tcgtctgctg cggtaagcgc agccgagggt gatgaatcag
     8461 taacgaccac tgttaatggc ggtgttattc attttaaagg tgaagtggta aatgccgctt
     8521 gtgcaattga ttcagaatca atgaaccaaa cggttgagct gggtcaggtt cgttcttctc
     8581 gcctggctaa agcgggtgac ctcagctccg ccgttggctt caatatcaag ctgaatgatt
     8641 gtgataccaa tgtttccagt aatgcagctg ttgcattcct gggtactact gtcaccagta
     8701 atgacgatac gttagcgctg cagagttcag cggcaggctc tgcccaaaat gtcggtattc
     8761 aaattttgga ccgtacgggt gaggtattaa tacttgatgg ggccactttt agtgctaaaa
     8821 ccgacttgat tgatggcacg aatatactgc cattccaggc tcgttatatt gctctcgggc
     8881 agtccgtagc tggtactgca aacgctgatg cgaccttcaa agttcaatat ctataattca
     8941 atcttaaggt ggctatacgt catctgaaat tgaaacaggg tgtgtgtggc atggctacgc
     9001 acacccgagc aatttaccaa tattcatctc atttttaatg agaaatggtt tgtatttgga
     9061 gcgctatgaa aaatcacata atatctaaac tatcaataaa caacagtagt taaacttact
     9121 gttatttaaa atatttactt tgtagcaagg ttttcctatg aaaagagaac caacatttat
     9181 tacaaacgca ttaagtctgg aaacccggcg actcgccaat gtattagcaa cattaatcga
     9241 ttgtctgatg taacggtttt cccgcttatc ctcttaacat atggagaatg catcagatgc
     9301 aaactacgcg taccccctac tctatttcct ttatggcgac agtgttactt ttattgctct
     9361 ttgcttgcca tagcaccgtt gccaacgcag ctgtcgcact gggtgcgacg cgggtaattt
     9421 atccggcaaa ccagaaacag gttttattgc ctgtcacgaa taatgatcct gccagtgtct
     9481 atctcattca gtcatggata gaaaatgcag gtgaccaaaa agacacccag tttgtcatta
     9541 ccccaccgct tttttccatg caaggtaaaa aagagaatac gctgcggata atcaacgcta
     9601 ctaatcatca gttaccaggc gaccgtgaaa gcctgttctg ggtgaatgtt aaagctattc
     9661 ctgctatgga aaaagaccag aagaatgaga atacattaca gctggcaatt attagccgca
     9721 taaaaatgtt ttatcgcccc acgaacctgg caatggcacc tgaagaagcg cctgcaatgc
     9781 ttcggtttcg tcgcagcggt agcaaactca cgcttattaa tccaacgccc tatttcatta
     9841 ccgtaacaaa tatgaaagca ggaaatagca atctcccaaa caccatggtg ccgccaaaag
     9901 gtgaagtatc ggtggatatc acgcatgctg cgaccgggga tatcagtttt cagacgatca
     9961 atgattacgg tgcattaact ccgcgtatta aggcaaccat gcagtaagcc agtgacctaa
    10021 ttaataataa actcaatcgg aattaataat gacagccatt cgtgccgcat ttaaggcata
    10081 tcgtatgcat caggtgttaa taatgcctcg ctttgcacgg cttaccattg cccttggctt
    10141 agcaactgcg gtatttcccg ttgacgcaga gtattatttt aatccacggt ttttaagcaa
    10201 tgatcttgct gaatctgtcg atttatccgc ctttactaag ggacgcgagg caccacctgg
    10261 tacatatcgg gtcgacattt atttgaacga tgaatttatg actagtcggg atattacttt
    10321 tattgcagat gataataatg cagaactcat cccatgcctg agtacagatc ttcttgtcag
    10381 tcttggtata aaaaaatcag cgttattaga caataaagaa cattcggcag aaaaacatgt
    10441 gccggacaac agcgcatgca caccacttca ggatcgtttg gccgacgcat caactgagtt
    10501 tgatgttggt cagcaacatt tgtcactctc cgttccgcaa atttatgttg gcagaatggc
    10561 tcgcggctat gtttctcctg atctgtggga agaaggaata aatgctgggc ttctaaacta
    10621 tagttttaat ggtaattcta ttaataatcg tagtaaccat aatgcaggaa aatcaaacta
    10681 tgcatatttg aatttacaga gtggcatcaa cattggtagt tggcgactac gcgataactc
    10741 aacgtggagt tataacagtg ggagtagcaa ttcatctgac agcaataaat ggcagcatat
    10801 caatacgtcg gctgaacgtg acattattcc cttacgctca cggttaacgg taggtgatag
    10861 ttataccgat ggcgatattt ttgatagtgt gaactttc
//
LOCUS       DAZKHC020000124        10891 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZKHC020000124 DAZKHC020000000
VERSION     DAZKHC020000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.124"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(136..753)
                     /gene="yncG"
                     /locus_tag="ACHOSM_RS20110"
                     /old_locus_tag="pgaptmp_004022"
     CDS             complement(136..753)
                     /gene="yncG"
                     /locus_tag="ACHOSM_RS20110"
                     /old_locus_tag="pgaptmp_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HGU4560414.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            1020..2519
                     /gene="ansP"
                     /locus_tag="ACHOSM_RS20115"
                     /old_locus_tag="pgaptmp_004023"
     CDS             1020..2519
                     /gene="ansP"
                     /locus_tag="ACHOSM_RS20115"
                     /old_locus_tag="pgaptmp_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HGU4560415.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2634..3695)
                     /gene="yncE"
                     /locus_tag="ACHOSM_RS20120"
                     /old_locus_tag="pgaptmp_004024"
     CDS             complement(2634..3695)
                     /gene="yncE"
                     /locus_tag="ACHOSM_RS20120"
                     /old_locus_tag="pgaptmp_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HGU4560416.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3937..6039
                     /gene="pqqU"
                     /locus_tag="ACHOSM_RS20125"
                     /old_locus_tag="pgaptmp_004025"
     CDS             3937..6039
                     /gene="pqqU"
                     /locus_tag="ACHOSM_RS20125"
                     /old_locus_tag="pgaptmp_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HGU4560417.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGKKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(6075..6740)
                     /gene="mcbR"
                     /locus_tag="ACHOSM_RS20130"
                     /old_locus_tag="pgaptmp_004026"
     CDS             complement(6075..6740)
                     /gene="mcbR"
                     /locus_tag="ACHOSM_RS20130"
                     /old_locus_tag="pgaptmp_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HGU4560418.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6938..7975)
                     /gene="curA"
                     /locus_tag="ACHOSM_RS20135"
                     /old_locus_tag="pgaptmp_004027"
     CDS             complement(6938..7975)
                     /gene="curA"
                     /locus_tag="ACHOSM_RS20135"
                     /old_locus_tag="pgaptmp_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HGU4560419.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8156..8674
                     /gene="mddA"
                     /locus_tag="ACHOSM_RS20140"
                     /old_locus_tag="pgaptmp_004028"
     CDS             8156..8674
                     /gene="mddA"
                     /locus_tag="ACHOSM_RS20140"
                     /old_locus_tag="pgaptmp_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HGU4560420.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8671..9120
                     /gene="ydcZ"
                     /locus_tag="ACHOSM_RS20145"
                     /old_locus_tag="pgaptmp_004029"
     CDS             8671..9120
                     /gene="ydcZ"
                     /locus_tag="ACHOSM_RS20145"
                     /old_locus_tag="pgaptmp_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4560421.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(9121..9354)
                     /gene="ydcY"
                     /locus_tag="ACHOSM_RS20150"
                     /old_locus_tag="pgaptmp_004030"
     CDS             complement(9121..9354)
                     /gene="ydcY"
                     /locus_tag="ACHOSM_RS20150"
                     /old_locus_tag="pgaptmp_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HGU4560422.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSRENRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9440..9613)
                     /gene="ortT"
                     /locus_tag="ACHOSM_RS20155"
                     /old_locus_tag="pgaptmp_004031"
     CDS             complement(9440..9613)
                     /gene="ortT"
                     /locus_tag="ACHOSM_RS20155"
                     /old_locus_tag="pgaptmp_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HGU4560423.1"
                     /translation="MSLYQHMLVFYAVMATIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9808..9903
                     /gene="yncL"
                     /locus_tag="ACHOSM_RS20160"
                     /old_locus_tag="pgaptmp_004032"
     CDS             9808..9903
                     /gene="yncL"
                     /locus_tag="ACHOSM_RS20160"
                     /old_locus_tag="pgaptmp_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HGU4560424.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(10305..>10891)
                     /locus_tag="ACHOSM_RS20165"
                     /old_locus_tag="pgaptmp_004033"
     CDS             complement(10305..>10891)
                     /locus_tag="ACHOSM_RS20165"
                     /old_locus_tag="pgaptmp_004033"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HGU4560425.1"
                     /translation="FSSVGAFSFPTCHFSGNIRIGRFCSIASNVKIMGGNHPLNRFTT
                     HMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGNDVVLKGGIAIGDGAV
                     IAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWWNYNYSDLPDNNKCDD
                     INYFVEEMNRLISDGNIQERDYKKFNLSEVFRGL"
BASE COUNT         2718 a         2623 c         2577 g         2973 t
ORIGIN      
        1 ggaaatgaaa tagacaagct gaatgaaggg gagagtgata taaatcaaaa aacataacat
       61 gaatttaatt aattacgccc atcattgtgg ataaagttac tgaataataa ataacataat
      121 cctctggtat cccactcaaa taatttcatt cctttttaat acttcttgta atttcggtaa
      181 ctgacataca gcatcagcaa tcgcactgat attcgtggca ttatcctgaa accattcatg
      241 cccaggcccc catgtgcgca tagtgcaaag ataacagtcc accagcgtta gttgctcacc
      301 aaacgcataa ggttcagcgg tcaattgtga attcagccag atataaagcg atttacgata
      361 ttcaataaca ttcttcttta actgttcagg tgcgtcagga gcccaacgct cagggtaatc
      421 ggcgaaagtg aatgttggat agacattagc aaccagccag accaatagcc gttgaaactg
      481 ctgacgttcg gcgcgcccaa ccggtggcgc aaggtccggg cgacgatcga gaaccatcaa
      541 tgcaatcgcc gctgtctccg tcataatctc gtcattttcc agcgccagtg tcggaatctg
      601 acacaacggg ttcagggttt tcaataactc gcgtgaagtt ccctcgtggt caaaaccact
      661 gacatcaacg aactgataag gaatatcagc cagtgttagc atcagctcac tgattgtaga
      721 gccccatccg ggtacgccat acaccttaat catatgcccc tcacaatctt ttaaagatta
      781 agtgtaggac agtacacagg ttaaccagca aaaggatttg tcattttttc gtcagttgta
      841 ttaatcccct tcaggattgt aaggggatgt tttgcatcgt ttatcgataa cgggtaggat
      901 gctgcgccga tagttttata cccataaaga ataatggtga taactatcat cgccaggatg
      961 aataaacatt gttcatggca acttatatga ctttttcatt aaagcaatca gggagagcaa
     1021 tgagtaaaca cgacaccgac acttcagatc aacacgccgc gaaacgccgc tggcttaatg
     1081 cccacgaaga ggggtatcac aaagcgatgg gcaatcgcca ggtacagatg atcgccattg
     1141 gcggcgcgat tggcaccggc ttgtttttag gtgcaggagc ccgactgcaa atggcgggtc
     1201 cagcactggc actggtttat ttaatttgtg gcttgttttc gttttttatt ctgcgtgcat
     1261 tgggtgagct ggtgctacac cgcccttcca gtggcagttt tgtctcttat gcccgtgagt
     1321 ttttgggtga gaaagccgct tatgttgctg gctggatgta cttcatcaac tgggcgatga
     1381 cggggattgt tgatattacc gccgtcgctc tgtatatgca ttactggggt gcgtttggcg
     1441 gcgtgccgca gtgggtcttt gcgctcgctg cacttaccat cgttggcacc atgaatatga
     1501 tcggtgtgaa atggtttgcg gagatggagt tctggtttgc gcttattaaa gtgctcgcca
     1561 ttgtgacctt tttggtcgtg ggtacagtgt tcctcggtag tggtcagccg ctggatggca
     1621 acaccactgg ctttcattta atcaccgata atggcggctt cttcccccac ggtttgctgc
     1681 ctgcgctggt gttgattcag ggcgtagtgt ttgcttttgc ctccattgaa atggtgggta
     1741 cagctgccgg agaatgtaaa gatccgcaga ccatggtgcc taaagccatt aacagtgtga
     1801 tttggcgtat tggcctgttt tacgtcggct ccgtggtgtt gctggttatg ttattgccgt
     1861 ggagcgcgta tcaggcgggg caaagtccgt tcgtgacgtt tttctctaaa ctgggtgtgc
     1921 catatatcgg cagcattatg aacattgtgg tgctgaccgc tgccctctcc agcctgaact
     1981 caggtctgta ctgcaccgga cgtattctgc gctcaatggc gatgggcggt tccgcaccga
     2041 gttttatggc gaaaatgagt cgtcagcatg tgccgtatgc cgggattctg gcgacactag
     2101 ttgtgtatgt cgtcggcgta ttcctcaact atctggtgcc gtcgcgcgta tttgagattg
     2161 tgttgaactt cgcgtcgctg ggaatcatcg cttcatgggc gtttatcatc gtgtgccaga
     2221 tgcgcctgcg taaagcaatt aaacaaggca aagcagcgga tgtcagtttt aaactgcctg
     2281 gcgcgccctt cacttcctgg ctgacattac tgtttttact gagtgtcctg gtgctgatgg
     2341 cgttcgatta cccgaacggg acttatacta tcgcggcgct gccgattatc ggtattctgc
     2401 tggttatagg ctggtttggt gtgcgcaaac gcgttgctga aattcacagc actgcgccag
     2461 tcgtcgagga agatgaagaa aaacaggaaa ttgtgtttaa gcctgaaacg gcgagctaat
     2521 tgcaaaattg cccggctacg tgccgggcaa atttagatta ctgacgaagg tgtgccgcat
     2581 gcggcgtgat aaaagtcagg caagtctctc cgttttcacc ctcgccacat ctattacagc
     2641 gcaatacgaa tcacatcgtc cggctgggta gcttcctgct gtttagtgga tttttgtttc
     2701 acactgacat acagcgtttt gccatcggca gacagcgcca ggctgtttgg atgagtcggc
     2761 gtatcgaacg ttttcaccac tttatagctt ttcgcgtcaa tcacactgac tttacctgcc
     2821 tgacgatgcg ttacgtaggc ttcattacgc gctgggttaa acagcacagc cagtgattct
     2881 ggtgccgcaa ccttcgccag aatattgcca ttacgggtat cgaccactaa cacttctgcg
     2941 gctttagaat cggtgataaa tgcacgctgc ctggcggtat caaggctaat gttgataaag
     3001 aagtgctctt tgccgtcatc cagcagcttt ttacggctga ggattttatt gtcggcggtg
     3061 tcgatggtaa tcaattcgcc gtcagcgtta gtggtgtaaa gacgtttgcc tttgctatcc
     3121 agcgccagac cggtactcat tttaccggtg ttctggatgg cggttttcag tttgatattc
     3181 tcgccatcaa cgacccaaat cacgctctct ttaccaatac cactgatgta aacggtgttc
     3241 gtggcatcat cagctaccag ctcacgcggt tgcagcgggc gcacctcttc cgtgcgctta
     3301 cgatcatcca gcaccagacg gcctttcacc tcgccagttt tggcatctat cgccgtgacc
     3361 gcgctgttta cggtgttacc aaaccacaac gtctgagtcg tgttattgat ggtggcacca
     3421 aacggcttga gatcgttatg gatcgcctgc gtaacttcca gagtgaccgg atcaagacga
     3481 taaaccacgc cacctttatc cagtttgcgg ctttgcgaag tggcgagcca cagcgcgttt
     3541 tcttgctggc tataagccat ttcgtaggca cctttaccta ccgctttacg cagcatttct
     3601 tctgcggcct gcgtactgaa tgatgaagca acaagcaatg aacctaacag taatgaacca
     3661 cgcaggcgcg atgaaaacag atgacgtaaa tgcatgacga ctccctttga tgaaccgata
     3721 tttttgctgc ttcacaatgc ttcgctaagc gtaatcatcg ttacgctctt gttataaatg
     3781 ggaatggtaa tcattatttt ctcaaatgtg gagcatttct tgccgttacg actacaattt
     3841 tctcaattac ttaacgtatt gtgccgtttc attttccata aatttacaat tttattacca
     3901 tgacggcgtt ttcccattct taacctggct tttttcatga agattgtttt cgttcgacag
     3961 accgttttgc ccgcactgct tgtcctttcc cccgttgttt ttgccgctga tgaacagact
     4021 atgattgtca gtgccgcacc gcaggtggtt tcagaactgg ataccccagc agcagtaagc
     4081 gtggtggatg gcgaggagat gcgcctggca acaccgcgca ttaacttgtc cgaatcactg
     4141 accggcgtgc ctggtttgca ggtacaaaac cggcagaact atgcgcaaga tttacagctg
     4201 tcgattcgcg gatttggctc ccgctccact tacggtattc gcggtattcg cctgtatgtg
     4261 gacggtattc ccgccaccat gcccgacggg caagggcaaa catccaacat cgatttaagc
     4321 agtgtgcaaa atgtggaagt gctgcgtggc cccttctctg ccctgtatgg caacgcgtct
     4381 ggcggtgtaa tgaatgtcac cacccagacc ggacaacagc caccaaccat tgaagccagt
     4441 agttactacg gcagttttgg cagctggcgc tatgggctga aagcaacggg cgcaacggga
     4501 gacggcacac agcctggcga tgtcgattac accgtctcaa ccacgcgttt tacgacccac
     4561 ggctatcgtg accatagtgg cgcacagaaa aatttagcta atgccaaact gggtgtacgc
     4621 attgatgacg ccagcaaatt aagcctgatt ttcaatagcg tggatatcaa agcagatgac
     4681 ccaggtgggc taaccaaagc agaatggaag gcgaatccac aacaagcgcc tcgtgcagaa
     4741 cagtacgaca cgcgaaaaac catcaagcaa actcaggctg ggttgcgcta tgagcgtagc
     4801 ctgagcgcgc aagatgatat gagtgtgatg atgtatgccg gagagcgaga aacgacccag
     4861 taccagtcaa tcccgatggc cccccaactt aacccgtcac atgcgggcgg cgtgattacc
     4921 ctgcaacgcc attaccaggg gatagacagc cgctggacac accgtggtga actgggcgtt
     4981 ccggtcacgt tcactaccgg cctgaactac gaaaacatga gtgaaaaccg caagggctac
     5041 aataacttcc gcctgaatag cggcatgccg gagtacggga aaaaaggtga gttgcgtcgc
     5101 gacgaacgca atctgatgtg gaacatcgat ccctatttac agacgcagtg gcagctgagc
     5161 gaaaaactgt cgctggatgc tggcgtgcgc tacagctccg tgtggtttga ttccaacgac
     5221 cattacgtta ctccgggtaa cggcgatgac agcggtgatg ccagttatca taaatggcta
     5281 cctgccggtt cgttaaaata tgcaatgacc gatgcctgga atatctatct ggcagccggg
     5341 cgaggttttg aaacgccgac gattaatgag ctgtcttatc gtgctgatgg gcaaagcggt
     5401 atgaactttg gtttaaaacc atccaccaac gatacaattg agatcggcag taaaacgcgt
     5461 attggtgatg ggctgcttag tctcgcattg tttcagaccg acactgatga tgaaattgtt
     5521 gtcgatagca gtagcggtgg gcgtacgact tacaaaaatg ccggaaagac ccgtcgtcaa
     5581 ggcgctgaac tggcatggga tcaacgtttc gcaggagatt ttcgcgtaaa cgcgtcctgg
     5641 acctggcttg atgcgaccta tcgcagcaat gtttgcaatg aacaggattg taacggtaat
     5701 cggatgccag ggatcgcccg taatatgggc tttgcgtcga taggttatgt accggaagat
     5761 ggttggtatg caggcacgga agcgcgttat atgggcgata ttatggcaga tgatgaaaat
     5821 acggcaaaag cgccgtctta tactctcgtc ggcttattca ccgggtataa atacaattac
     5881 cacaatttaa ctgtggattt atttggtcgt gtcgataatt tattcgataa agaatacgtt
     5941 ggttctgtca ttgtcaatga gtcaaacggg cgatattacg aaccttcgcc cggacgaaat
     6001 tatggtgtcg gcatgaatat tgcgtggaga tttgagtaat taccttgatg cccggtattt
     6061 gccgggcatt tactttaacg attgtattgc tggtataaaa tagcaatatt ttgttgcata
     6121 atttcagcaa gacaatgtct gcatccctct ttgtcttttg cttttaacgc agcgagtaac
     6181 agatgatagt tttctatatg ctcccgtaat tccgctggat taatcgcttc atagagataa
     6241 tgtaaaccag gccccatcct gacccacagt tgctcaatca tctcacacag gatgggcata
     6301 tttgagcgat gataaatcgc taagcgaaat agcctgttta cattaatgat ttgttccatg
     6361 tcgccctttt cttgcgcctg ctgtaatttc tcgagcaatt cctggagttc cgccaggtct
     6421 tgcggggtga ggttttcaac agccagagca actgccatta attccagttc gtagcggatc
     6481 cgattgattt cgtctaattg acgtttcccc acttccggaa ctgtaaatgc ttgtgcaggt
     6541 gcgacagaaa gcgcattcac cgaaaccaga cgtaataatg cttcacgcac aggtgtaata
     6601 ctcataccta attgctccgc cagattttta gtaatcaggc gtgcgccagg ttttaatgcg
     6661 ccaatactaa gctgatgttt caggtcgttc tcaacctgca gagtcaaact gacatgtttc
     6721 atttttccca ttccaggcat tttaattcct tcgcgtgttt tctgtccaac cctgcctgca
     6781 cggcagaagg agttcacctg gtgtattaaa gtgaataatc ataaaccacc cacctttgtt
     6841 gttattagca gcagaacaat gacgtggtta gttaattaaa gctgaaaata agctttttgc
     6901 ctggattttt atggcggctt acgccgccgt tgaaacttta atcatcaccc gcgacgcgga
     6961 tcaccacttt gccgaagttt ttacccttca gcaggccgat aaacgtctgt ggcgcattct
     7021 ctaaaccatc agtaatatct tcgtggtagt ggattttatc ctctttcacc cattgcccca
     7081 tctccttctg aaactcatgg atgcggtgac cataatcctg agcgataata aacccttgca
     7141 agcgaatacg ttttttcagc accgttgcca tcaacagggg taaacgatcc ggaccgggtg
     7201 gtagctctgt agcgttatag ctgctcacta agccacaaac gggaatgcgc gcagatgtat
     7261 taagtaatgg tagcactgca tcaaatacct taccgcccac gttttcataa tagatatcaa
     7321 taccttttgg gcacgctttc accagttgtt cggcaaaatc atccgcgtgg tgatcaagac
     7381 aaacatcgaa gcccagcacc tcgatagcat ggcggcattt ttccgcgcca ccggcaaccc
     7441 ccaccaccct gcaaccttta agtttgccga tttgccccac cgttgctccc acaggacctg
     7501 tcgccgcagc taccaccaac gtttcgccct ctttaggctg accgatatcc agcagcccca
     7561 tataagcggt aaagcctggc atccccagca cacccagcga ccacgatgga ttttgcggat
     7621 gatcgccaag tttcaccaga tcatcaccac tggatatgtc atagtcctgc catccactgt
     7681 agcccagcac ccagtcgcca ggctgataat caggatgatt cgactccaca acacggctca
     7741 ccgtgccgcc gaccatcacg ccgccaatat caacaggcgg tgaataagat ggctcatcgc
     7801 tcatacgtcc acgcatatac gggtccaggg acaaataaac tgtgcgcagt aacacctgtc
     7861 cttcacccgg tgtggcgaca tcatcttctt caagacggaa attctccgga accggtgcgc
     7921 catgtggacg cgaggccaga acccaacgtc gattacgctg cttttgttgc cccataatgc
     7981 tctcctgtgc ttttggcatg aatccatcaa tagtagcagg ctcacgggat tggttttgtt
     8041 tgcagccgtc acatcccctc tgcaacacgc aataatttca tcgaggaatc cgttgcttcg
     8101 ggctgttttc gatagtattg ataacatcat catcattatt ttatcgaggc cagacatgtc
     8161 catccgtttt gcccgcaaag ccgattgtgc tgccattgcg gaaatttata accacgccgt
     8221 gttgtatacg gcggctatct ggaatgacca aacggttgat gcagataacc gcattgcctg
     8281 gtttgaagcg cggaccatag caggttatcc ggtgctggta agtgaagaag atggcgtagt
     8341 gacgggatat gcctcgtttg gcgactggcg tagtttcgat ggttttcgcc ataccgtgga
     8401 acattcggtt tatgtccatc ccgatcatca gggcaaaggt ctggggcgta aattgttaag
     8461 ccgattgatt gatgaagcgc gggatagcgg gaagcatgtc atggtcgccg ggatcgaatc
     8521 gcaaaatcag gcctcgctgc atctgcacca ttcgctggga tttgtcgtca ccgcacaaat
     8581 gccgcaggtg ggcactaaat ttgggcgctg gctggatctg acatttatgc agttgcaact
     8641 cgacgaacgc actgaaccgg acgcgattgg atgaatcagt cgctcaccct tgcttttttg
     8701 attgccgccg gaattggtct ggtagtccag aacacgctga tggtgcgcat cacccagacc
     8761 tcctctacca ttctcatcgc catgttgctg aactcactgg tggggattgt gctctttgtt
     8821 tctattttat ggtttaaaca aggcatggcg gggtttggcg aactggtgtc cagcgtgcgc
     8881 tggtggacac ttatacccgg cttgctggga tcgttttttg tcttcgccag tatcagcgga
     8941 tatcagaatg tgggagcggc gacaactatt gcagtactgg tcgccagcca gctaattggc
     9001 ggtctgatgc tggatatctt ccgcagtcat ggcgtaccac tacgggcgtt gttcggccca
     9061 atttgtggcg caattttact tgtggttggc gcctggttgg tagccagacg ctcattttaa
     9121 tcagaggatg gtgccgcctt tggttaactg ttcgtggcgc gcttccatat cttctttatg
     9181 cttgcgaccg tgatgagcaa tagcggtgcg caaacgctgc tgctgggtgt agcgattttc
     9241 ccgacttaac tccgcgtcat cgctcagggc gattaataat tcgttcatat gggcaatgac
     9301 gctctcttca atcgcggcat ccacgcgcgc gatgacttca tccagatgcg acatactttc
     9361 tccttaacca cttgtgaaat ttatcgattc atccaaatga tataagtctg cgtggtgccg
     9421 tcaagccagt gagtaagatt taaaacagtg aaaacaacag cgccaccggg aaactcatcg
     9481 gccatgttgc ccccaccaga aaggcgctta agaaacggat gcgtttctta tcgtgagaaa
     9541 gaaaccaggt gataagaaat gcgattgttg ccataaccgc ataaaaaaca agcatatgtt
     9601 gatagagaga catgtacggg cgtccagcca gatgaataaa acgcgcgcaa atatgcgtca
     9661 ttttttgacg tgcatcaagt tttttcacca gtttgagaaa aaatcatcaa acagcaactt
     9721 gcccttatcc atcttcagaa tgagtattat tgcggatttt cttaacccgt actatacaca
     9781 taggggcaat gataaaaggt ggcaaaaatg aatgtttcca gtagaactgt agtactgata
     9841 aatgtctttg ctgctgttgg tttgtttact cttatctcta tgagatttgg ctggtttatt
     9901 tgatgtagtg attttgcacg cagcacgtac tgtcagagaa atcaactctc tgacagtacg
     9961 caagatcttc tcatactctc caggtttaca acattatcct taatatttac ttctttttgc
    10021 tggcatctac attgaaatga gtagaaaaca tccccctact gtcgtctata caaacagctt
    10081 actgattcaa acggaatctt gttttcacat ttttctatca tgaatacttt atacgcgcgg
    10141 gagtctataa atgctttata ctttcaagga ataacgtaaa tataaaaaga tggcgtaaaa
    10201 tatatgcatg gcaggatgtt tgcaacctcc ttgatgacgc tgccccattg aaattgcaaa
    10261 acccgccctg gttatcttgc ccatttatca tgcaaaacag ataattatag gcctctgaat
    10321 acctcagaaa gattgaattt tttatagtcc ctctcttgaa tatttccatc tgatatcaat
    10381 cgattcatct cttcaacaaa ataatttata tcatcacact tgttattatc cggaagatct
    10441 gagtagttat aattccacca ttttattctc aacagttcat caataacatt tgaatcaaac
    10501 ctaaatctga ttatttttgc aggaactcct gcaactatgg catatggggg cacatctttg
    10561 gtaacaacgg aattggcagc aataacagca ccatcaccta ttgcgatgcc acctttcaat
    10621 acaacatcat ttcctatcca gacatcattt cctataatcg gattttcagg tttggtaatg
    10681 aatggtttca atgtccagct acgttcaaat tcactcatag caaacttatc aaattcaccg
    10741 ttataagtca tcatgtgagt agtaaaacga ttcaaaggat gatttccacc cattattttt
    10801 acatttgatg caatactaca aaatcgacct atacgtatat tccctgaaaa atggcaggtc
    10861 ggaaatgaaa aagcgccgac actggagaaa t
//
LOCUS       DAZKHC020000125        10870 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZKHC020000125 DAZKHC020000000
VERSION     DAZKHC020000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.125"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            85..801
                     /gene="nanC"
                     /locus_tag="ACHOSM_RS20170"
                     /old_locus_tag="pgaptmp_004034"
     CDS             85..801
                     /gene="nanC"
                     /locus_tag="ACHOSM_RS20170"
                     /old_locus_tag="pgaptmp_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HGU4560426.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            821..1927
                     /gene="nanM"
                     /locus_tag="ACHOSM_RS20175"
                     /old_locus_tag="pgaptmp_004035"
     CDS             821..1927
                     /gene="nanM"
                     /locus_tag="ACHOSM_RS20175"
                     /old_locus_tag="pgaptmp_004035"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HGU4560427.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKRWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAYYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGDNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSADIHLWYNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVMDSVLISVKDNKVTVQN"
     gene            1992..2972
                     /gene="nanS"
                     /locus_tag="ACHOSM_RS20180"
                     /old_locus_tag="pgaptmp_004036"
     CDS             1992..2972
                     /gene="nanS"
                     /locus_tag="ACHOSM_RS20180"
                     /old_locus_tag="pgaptmp_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4560428.1"
                     /translation="MNAIISPDYYYILTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDKFVEAILQFWRER"
     gene            complement(2980..3630)
                     /locus_tag="ACHOSM_RS20185"
                     /old_locus_tag="pgaptmp_004037"
     CDS             complement(2980..3630)
                     /locus_tag="ACHOSM_RS20185"
                     /old_locus_tag="pgaptmp_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4560429.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHLDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCDVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVYPNCHAMLHRRKPPFTPEQLKALMDANKSN"
     gene            3767..3910
                     /locus_tag="ACHOSM_RS20190"
                     /old_locus_tag="pgaptmp_004038"
                     /pseudo
     CDS             3767..3910
                     /locus_tag="ACHOSM_RS20190"
                     /old_locus_tag="pgaptmp_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            4009..4194
                     /locus_tag="ACHOSM_RS20195"
                     /old_locus_tag="pgaptmp_004039"
     CDS             4009..4194
                     /locus_tag="ACHOSM_RS20195"
                     /old_locus_tag="pgaptmp_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560430.1"
                     /translation="MSNKNYESHRKAIVSKGIPPALLNRLTNSDVQVINTFLTRVSKL
                     ELSQQEKDWIIKIISMV"
     gene            complement(4574..4702)
                     /locus_tag="ACHOSM_RS20200"
                     /old_locus_tag="pgaptmp_004040"
                     /pseudo
     CDS             complement(4574..4702)
                     /locus_tag="ACHOSM_RS20200"
                     /old_locus_tag="pgaptmp_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(4678..4827)
                     /locus_tag="ACHOSM_RS20205"
                     /old_locus_tag="pgaptmp_004041"
                     /pseudo
     CDS             complement(4678..4827)
                     /locus_tag="ACHOSM_RS20205"
                     /old_locus_tag="pgaptmp_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
     gene            complement(4912..5109)
                     /locus_tag="ACHOSM_RS20210"
                     /old_locus_tag="pgaptmp_004042"
     CDS             complement(4912..5109)
                     /locus_tag="ACHOSM_RS20210"
                     /old_locus_tag="pgaptmp_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU4560431.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIHTLRQLQLLQQNR"
     gene            complement(5121..5609)
                     /locus_tag="ACHOSM_RS20215"
                     /old_locus_tag="pgaptmp_004043"
     CDS             complement(5121..5609)
                     /locus_tag="ACHOSM_RS20215"
                     /old_locus_tag="pgaptmp_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4560432.1"
                     /translation="MNLSLTLEADSVNVQALNMGRIVVDVDGVNLAELINVACDNGYS
                     LRIVDESDRTSAERTPPFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTGAWSYPVLWLKRLGLSKTFRRLVVTLTRRYGVSLIHLDANAECLPGLPTF
                     DW"
     gene            complement(5606..5986)
                     /gene="cbtA"
                     /locus_tag="ACHOSM_RS20220"
                     /old_locus_tag="pgaptmp_004044"
     CDS             complement(5606..5986)
                     /gene="cbtA"
                     /locus_tag="ACHOSM_RS20220"
                     /old_locus_tag="pgaptmp_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HGU4560433.1"
                     /translation="MKTLPVLPGQAASSCPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGRHPEEAKQ"
     gene            complement(6075..6443)
                     /locus_tag="ACHOSM_RS20225"
                     /old_locus_tag="pgaptmp_004045"
     CDS             complement(6075..6443)
                     /locus_tag="ACHOSM_RS20225"
                     /old_locus_tag="pgaptmp_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4560434.1"
                     /translation="MSDTLSGTTHPDDNNSHPWWGLPCTVTPCFGACLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPEMKN"
     gene            complement(6523..6744)
                     /locus_tag="ACHOSM_RS20230"
                     /old_locus_tag="pgaptmp_004046"
     CDS             complement(6523..6744)
                     /locus_tag="ACHOSM_RS20230"
                     /old_locus_tag="pgaptmp_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU4560435.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGSEVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(6807..7283)
                     /locus_tag="ACHOSM_RS20235"
                     /old_locus_tag="pgaptmp_004047"
     CDS             complement(6807..7283)
                     /locus_tag="ACHOSM_RS20235"
                     /old_locus_tag="pgaptmp_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HGU4560436.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(7299..7784)
                     /locus_tag="ACHOSM_RS20240"
                     /old_locus_tag="pgaptmp_004048"
     CDS             complement(7299..7784)
                     /locus_tag="ACHOSM_RS20240"
                     /old_locus_tag="pgaptmp_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4560437.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWLLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(7839..8657)
                     /locus_tag="ACHOSM_RS20245"
                     /old_locus_tag="pgaptmp_004049"
     CDS             complement(7839..8657)
                     /locus_tag="ACHOSM_RS20245"
                     /old_locus_tag="pgaptmp_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4560438.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRTIHSIDTDIKLNRALWVMAETLLKNLR"
     gene            complement(8757..8990)
                     /locus_tag="ACHOSM_RS20250"
                     /old_locus_tag="pgaptmp_004050"
     CDS             complement(8757..8990)
                     /locus_tag="ACHOSM_RS20250"
                     /old_locus_tag="pgaptmp_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGU4560439.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            complement(9069..9524)
                     /locus_tag="ACHOSM_RS20255"
                     /old_locus_tag="pgaptmp_004051"
     CDS             complement(9069..9524)
                     /locus_tag="ACHOSM_RS20255"
                     /old_locus_tag="pgaptmp_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HGU4560440.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            complement(9600..>10870)
                     /locus_tag="ACHOSM_RS20260"
                     /old_locus_tag="pgaptmp_004052"
     CDS             complement(9600..>10870)
                     /locus_tag="ACHOSM_RS20260"
                     /old_locus_tag="pgaptmp_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560441.1"
                     /translation="GNHIVDRVRLKNARILGDNNARNGADRLVSGTEIQTKYCSTAAR
                     SVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVPGVTDPAEASR
                     LIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASVFWLSTGDRD
                     AALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPTVRLALQKGTG
                     AGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGTQFIRNLAVASSGVAGGA
                     VGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQE
                     QVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSII
                     RQRPVMEYDASHLGNMVNRLEEALPPELPA"
BASE COUNT         2787 a         2624 c         2750 g         2709 t
ORIGIN      
        1 gcaaaaaaat attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct
       61 gtatcctaca aatgagtgaa atttatgaaa aaggctaaaa tactttctgg cgtattatta
      121 ctgtgctttt cgtccccatt aatttctcag gctgcgacac tggacgtacg tggtggatat
      181 cgtagtggaa gccacgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga
      241 tggtgggcaa gcatggaaag taatacctgg aataccattc atgataataa aaaggaaaat
      301 gccgcactca atgatgttca ggttgaagtt aattacgcga ttaaacttga tgatcaatgg
      361 acggtgcgcc cgggaatgtt aacgcatttt agcagcaatg gcacacgcta cggaccctac
      421 gtaaaactgt cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac
      481 tggaaagctt accgacaaca agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt
      541 tgggatggat atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg
      601 ctatacagta aacagaacga ttatcgctat gcaaaccata agaaatgggc gacggaaaat
      661 gcatttgttc tacaatacca tatgacgccc gatattacgc catacataga atatgactac
      721 cttgaccgtc agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt
      781 ggtgtgtcat ttaaactgta gtagacagga gacagtcaca atgaataaaa caataacggc
      841 gcttactatc ataatggctt catttgccgc aaacgcgtct gtattaccgg aaactcctgt
      901 gccatttaaa agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag
      961 cgcaggtacg gcatggtaca agctggatac acaggccaaa gataaaagat ggacagcgtt
     1021 agctgcattc cctggcggac cgagagatca agcaacctct gcatttattg atggcaatct
     1081 gtatgtgttt ggcggcattg gcaaaaacag cgagggcttg actcaggtat ttaatgacgt
     1141 acacaaatac aaccccaaaa ccaatagctg ggttaaattg atgtcgcacg cgccgatggg
     1201 catggcgggc catgtgactt ttgtacacaa cggcaaggct tatgttactg gcggtgttaa
     1261 ccagaatatc ttcaatggct attttgaaga tctcaacgaa gctggaaaag attcaaccgc
     1321 tatagataaa atcaacgcct actattttga caaaaaagca gaagattatt tcttcaataa
     1381 gtttctgttg tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatctccctg
     1441 gtacggaacg gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc ttattaatgg
     1501 cgaagccaaa ccaggattgc gaacggatgc cgtatttgaa cttgacttca ccggtgataa
     1561 tttaaaatgg aataagcttg atcccgtctc atcaccagat ggcgtcgctg gcggttttgc
     1621 ggggataagc aatgattctc ttatatttgc cggaggggcc ggattcaaag gttcacgaga
     1681 aaattaccag aacggtaaga actatgcgca tgaaggcctg aaaaaatcat atagcgctga
     1741 tattcatctt tggtataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc
     1801 ctacggagta tcattgccct ggaataatag tttattaatt attggcggtg aaactgcagg
     1861 cggcaaagca gtgatggatt cagttttaat ttctgtgaag gataataaag tcacagtaca
     1921 aaactaacgc ttcagggccc cggtaaaggg gccctgctat caatttgtca tttcaattac
     1981 gcgaaattta tatgaacgca ataatatcgc ctgattatta ctatattctt accgttgctg
     2041 gtcagtctaa tgccatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc
     2101 ctcatcccag aattaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgtcat
     2161 gtcactttaa cgacattatt ccactgactc actgcccaca cgatgttcag gatatgcagg
     2221 gttatcacca tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac
     2281 tgcatatagc acggaaatta ctgcccttta ttcctgataa tgcaggggtt ctcatcgttc
     2341 cgtgttgccg tggcggatcg gcttttaccg cgggcagcga agggacatat tcagaacggc
     2401 acggagccag ccatgatgct tgtcgttggg gaacggatac tccgctatac caggatttag
     2461 tcagcagaac gcgagccgca ctggcaaaaa atccgcataa caaattcctc ggcgtatgct
     2521 ggatgcaagg cgaatttgac ttaatgacca gtgactacgc gtcacaccct caacacttta
     2581 atcatatggt tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata
     2641 ttactgacgc accgtggttt tgcggcgata ccacctggta ctggaaagaa aatttccctc
     2701 atgcgtatga agttatttat ggcaattatc aaaataatgt tttagccaat attattttcg
     2761 tcgacttcca gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gacccggacg
     2821 atttaagcac gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac
     2881 tgcgaagcag tcatttcagc acggcagccc gtcgggggat tatttctgac aagtttgtag
     2941 aagcaatttt gcagttttgg cgcgaaaggt gagcgttcat taatttgatt tattcgcatc
     3001 catcagcgct tttaactgct ccggcgtaaa cggtggtttg cgtcggtgca acatcgcatg
     3061 gcaattaggg tacacaggca gcaaatcatt aactggatcc agctggtaat cctctttaat
     3121 ggctgacagc ggcaccaggt gatgcacatg gataaagcct tcagcgatat caccatagat
     3181 cttcaccaga tcaacatcgc atacgttgca ctggcatcca tgatgttcga ccgctttatc
     3241 tcgggccttt ttatcgcgtt catagcggtt tactgtcacc tttatcgccg caccttcaag
     3301 gtatgtctcc tgcgacggca actcatccgg gaacagaacg tcggctatgg gagccgcagc
     3361 ggctttatcc agatgaatat gaaatccctg ctctatgagt agcttgacac atttagattt
     3421 tattccgccg gtgaaatccg caggggtaaa ttgcgtcccc gtcatcaatg tcgctgcgat
     3481 gccagcaatc gccttcgagg ggtaacgtcg gccatgatat tcaatctcat acaaccgcgc
     3541 aggctggaac tgatgagttt ctcccgcatc ccataaatgg atagcttcga tgatataccg
     3601 ccgcggaaga tgatcaggga gtttattcat ggagaatgtc cttatacgtt ctcagaacag
     3661 gcagcaaaaa aacaaatgag atcaaggtaa tatatcttca cttgcaggct tgaacagcag
     3721 aaaatttgcg agcatgatcg caattatttc actgccaggg atatcaatgg cttccagtaa
     3781 atcgctacag caggcaattg ccaatataaa aatctggcac aagggtgaac agcgcgcgcc
     3841 gcataagcca ttgttattgc tatacgtatt agcgggatac ctgaatggac atccgcgcct
     3901 tttcgattat tgtagtggaa cagtctacga ggatgcagtt cgaagtgagt aagtaaaggt
     3961 tgctggaaac gatcaatgcc gccatacaat gatttttgag gatcaacaat gagtaataaa
     4021 aactacgaaa gtcaccgcaa agcgattgtt agtaaaggta taccacccgc tctattaaat
     4081 aggctcacca attcggatgt tcaggtgatc aataccttct taacacgagt gagcaaactg
     4141 gaactatctc aacaagagaa agactggatc ataaaaatca tctctatggt ttagacaatt
     4201 tcagccttgt agcctttcaa gggttttgtg tgggtacaaa cattatcgaa tcgggatata
     4261 gccgcagctg tagctgtatc cacatcagta agttgatgta ggttgactta ggttgagcga
     4321 aaaatagcga aaagccttgt gtggcgcgga tttcagtcat aaaaaaacgt ccgttgatgt
     4381 ctattgatgt tccgatggtg cgaaggtgcg aaggtgcgaa ggccggactc aaacatgtga
     4441 cttaacctca tgatttaaaa ttgttaataa taaacaatat catcttatac caacacagat
     4501 accaacacga aaaatcactg gttttcggca tcgaaccagg acaatcagct cctgattgag
     4561 ataccgagct ctgttactcg tcatcttcct cgacaggctt cattgcatca agtagtactc
     4621 gcaccatcgc gatagaacca atccggataa aagcatcttc tccatctata tctgttaact
     4681 gatatcgctc acttgatgcc ggataattgt gtcgatttgc agcagtgaac tctttcgtac
     4741 agccagaatg cggcggatgc tggttgagca gataacgtcc gtattcccgt tgaccacttc
     4801 cggacggtag gctgtgatat cagacataat cgatattcct ttttgcagat aaatgaaaat
     4861 gccagccctg ttcaggctgg cgtcagtgga atgaagtctg gtgagtaaga ctcaccggtt
     4921 ctgttgcaga agctgcagtt ggcgcagggt atggatatct tccctagcct gttcaatgca
     4981 gggcagcagt gctgccgaat ccgttttatc ctcattgttg tcaaagataa tccccgattc
     5041 gcagtcgata ttgtcctgca gccacgcaat cagaatatcc agcgctgttt ccgtggttaa
     5101 tgatttcatg ttgtttctgg ttaccagtcg aaagtgggta aacccggcag gcattcagcg
     5161 ttggcatcca gatgaatgag actgacgcca taacgtcggg taagtgtgac aaccagacga
     5221 cggaacgttt ttgacaggcc caggcgctta agccacagaa ccgggtacga ccacgcaccg
     5281 gtacgtaaca gatatccgct acctgtgaaa tgaatccatt ctgattcgcc ggtgtcattc
     5341 gtctgatgtg acagcgaata caaccaggcg ttgtcctttg ccgtgatatg tgcggtactg
     5401 cagcgtatgc cggtcagggc agcaaatggt ggcgtgcgct ctgctgatgt ccggtcagat
     5461 tcatcaacaa tacgaaggga gtagccattg tcgcaggcca cgttaatcag ttcagcgaga
     5521 ttaacaccat cgacgtcaac gacaatgcgt cccatgttca gcgcctgcac gttaacgctg
     5581 tctgcttcca gcgtcaggga aaggttcatt gtttcgcctc ctccggatgc ctgcccaggg
     5641 taatgttatt taccgttctg taattgtcgc gggtcatcag gccggttgcc ctgcgggccc
     5701 ggaggatatc gatgctgttt attaactgag agcgggtaca ggcactgaat tccggctggt
     5761 cggtacgcac cagtgcgtat ttttcaacaa gaaagttcac tgcatcgcac agtgaaatgc
     5821 cggcctcaat atgctgctca atcacacgtt catcagcgaa cggagtgtca ttcagcgtga
     5881 ggccgtagtg ctggtccagc agtcgggtga gcagtgtctg ccagatttca acaggagacg
     5941 ggcaagaact ggccgcctgc ccgggtaata caggtaatgt tttcatactg aagattttcc
     6001 tgatatgcag atataaaaat ggaaaagtgg cgtggtgaaa acaccgggcc gtagcagaag
     6061 gctattctgg agagttaatt tttcatttcg ggcgtcggat aaacagccag ataaacgtaa
     6121 ccacaggagc cgagggtgtc ggcttcacag gtcagcccct tcgcatacag tgtcacggta
     6181 tgctgatggc ggggattcag ttcaccgctg gtgagcatga gctccagttg tttcatcagc
     6241 agcggaaagg cctggtccag atggtacgca tccgcgtcgc tgaaccggcc tctgataccg
     6301 gcgcggtcgg caagataatg caaccggtta ccctcctgca ccagacaggc cccgaaacag
     6361 ggcgtcacgg tgcagggcag cccccaccag gggtggctgt tgttgtcatc gggatgagtt
     6421 gtcccggaga gtgtgtctga cacgataaag tcctcacaga aaatcggtta agaacggtcc
     6481 ggtaatggcg ataattctgc tcatcagaat gcccttactg actcagttca gagtgacgct
     6541 catcagccgg acatacgggc caaaactgtc cttacggcgt tcagcaaaca cggccagcac
     6601 accgggaata tcctgtactt cactaccggt atacgcctca gcactgccgt gccagcggta
     6661 tttgccggta cagaaaggaa aaagacggga tgccggatgt tgttggtgaa tacgcatggc
     6721 ttcaccacgg gtgatgattt tcatagtgtg atacctctga agacagaaga taaaagtgaa
     6781 aacaggtgtg atgtggttgt gacgggttaa agcagaccat gttcggcaaa ggagaaaacc
     6841 tggttgccac cgactatcag atggtccggc acccggatat ccaccaggcc cagtgcctgt
     6901 accagacgtt ccgtgataag gcggtctgcc ttgctgggtg tgacttcacc ggacgggtga
     6961 ttgtgcgcca gcaccacggc agcggcattg tggtacaggg cgcgtttaat cacttcccgg
     7021 ggatgaactt ccgtgcggtt gatggtgccg gtgaagaggg tttcaccggc aatcagttga
     7081 ttctggttat tcagatacag cacccggaat tcttcacgct ccagtcccgc catgttcaga
     7141 atcagccatt cccgtgccgc acgggtggag gtgaaggcca cgccgggttc atgaagatgg
     7201 cggtccaggg ttttcagggc ccgcagaatg agactgcgct cgccgggcgt catctctccg
     7261 ggcagaaagg aaagttgttg cattgtgctt ctctccattc agtcgatgat gcgcataatg
     7321 gcgctgcatt ccggatgctg cagggcgtaa tcccgcaacc ggtaataatg gaccgtcatg
     7381 gcataacact ctgtacgaca ggcatgatga ctgtacgtca tcagacaggc ggcaatgccg
     7441 gcggcttccg ggctcatttc agcgcggtta ccgttcatgg cattgaacag taaccagttt
     7501 tcgccatcat cgtcatccgg ttcgggggcc ataaatgccc cgccgttgtt cagagtgtac
     7561 agattccaga taccaccgca gtagtcttcg cacagacggt ccatccagcc gaaaacacgg
     7621 ggctccaggg tcacccactg tggaatgagg ccaaaatgct gcggccagaa gctgatgcgc
     7681 tgttcatcag ggactatggt ggcaaccagc tgaggctggt cattccctga tgcagcggtt
     7741 acggaaacag aagatgtggt ggaattatgt gaaatggttt tcatgaaatg actccttaaa
     7801 ggacaaataa atgcgtggtt ttcagggtaa tggctgaatc agcgcaggtt cttcagcagc
     7861 gtttccgcca tcacccacaa tgcgcggttg agcttaatgt cagtgtcaat gctgtgaatg
     7921 gtacgggtat ggatacgttt tcctttagcg ctgcgacctg aaattccgcc tttcagcata
     7981 ttctcctgaa tggtctgata agcactccac aggtccttac cgtaatcctc ccggcgtcgt
     8041 ggcgtcagaa tgtcggcggt ggtgacgggc tgatgttcgt caccataacg gtaagtcagt
     8101 gccgcctgtg ccagcgcctg gcgtgccggt ggcggcagga ccagcgactg catggcatca
     8161 cgtttctctt caatccggtc aaaaaccccc accacctcgt aagccccttc gataactttc
     8221 tccactacat ttccccggtg tggaacacgc acttccccca gagactggcc acagacgcac
     8281 ccgttctggc agacgaacct gaagtaaccc ggcagcatct ggtagctgga ggtaccgtca
     8341 tgagagttga gcagaataat ttcagggaca tgttctccgt ttatctctcc ggcccgccgc
     8401 agacgcagca tgtgttttgt gtatccccgg cggcccgggt cgcgcacacg ggtctggcag
     8461 gcgaagaatg gctgaaagcc ttcccgctgc aggctttcca gtacggtgat ggtggggatg
     8521 tacgtatagt tctggcttcg ggacgtgtgt ttctcttccc cgaaaatccc cggcacatag
     8581 tgcatcagtt cttcgtgtgt cagcggacgg tcacggcgta tctggttcgc ataaccaaaa
     8641 cgactggcta gtcgcataat ttgctcctta tcggtggtta agatttactg gtgtaataaa
     8701 tgaaaaagcc acgtctcccg gagaagacgc ggcctgatgg gtgaaatgaa tgacgtttat
     8761 tgtctgagaa gaccgtcact ggcgagccgc gtattaagca cctttccggc atcgggttca
     8821 gaattccggc agcgccagcg tcgctgacct tccgcgttgc ggataacccg acggaaatgc
     8881 attcccgggt catcctggat aaacacattg cggtaagctg ttatgacagc aaccgcctgc
     8941 ccgtatgaga aagatccttc agccaggaca tactctgtgt aacccggcat atctgttttc
     9001 tcctgataaa taaaaagcct ctgccacgcg ttgtgacaga ggctgaagca tgaaactgac
     9061 ttcagggatc agttaacgtt ttttccggaa actgtaatca gcagtggatg gtagtcctgg
     9121 ggattgaaaa ccgataacgg cagactgaca cgatggccgt tactttcttc agttgcttta
     9181 atgatttcgg ttgtggcgac attttccacg cactccgttt ccagaaatgc gtctgtggtt
     9241 cgcgtggcat tactgtcacc aaaagcttcc gtttccattt ttctggtcac cagcgtctga
     9301 ccatatttgt ctttgagttg cagagtgatg gtgagggggc caaatccttc atcgtttccg
     9361 ccattatcca gccggaactg gtaagcacaa atatttcccg ggagccatat cgtatctgta
     9421 ttgcgtatac tgatgtaacg ttgatcctgt gcccggagtg gggcagacca cgttaacccc
     9481 agaatgaagg cggtaatcat gcaggttttg aacaggtgaa tcatggtatt tacctctctg
     9541 agtcatgacg atgacactga caaatcaggt gataaagcgt aaaaggcgca gaatagccgt
     9601 tatgccggta actccggggg taatgcttct tccagtcggt taaccatatt gccgagatgg
     9661 gatgcatcat attccatgac ggggcgttgc ctgatgatac tgaccaccag tggtttgatt
     9721 aacatattgg tcgcggcccg ttgttgtata ccggcggcga aaatgatctc cagagcattc
     9781 tgatcgataa cacgggccag attctcgttc agattttcaa tctcatgtcc ggtcagcagg
     9841 aaactgccgg caagccatgt cacctgctcc tgaatcattg ccagaatttt gacgcgatct
     9901 tcttcaacca gcgctccggc aatttttcct gatacagcgg aggcaatcat tccccccaga
     9961 acaccgccaa ccacacgccc tgtcagtgca ccaaatggtc ccagtggact gaacagtatt
    10021 ccgcccgcca ctgacccgac agcaccacct gccacaccgg aagatgccac ggcaagattc
    10081 ctgatgaact gcgtaccgga gatccgtccc cgcaacattt tgatcatgtc agggccggtt
    10141 gtgacagcta ccagtgccag agatgtcacc agcgtaccct tcatcacttt gttcagggca
    10201 ctgatatttc ctgcaccggt ccccttctga agagccagtc ggacagtcgg gctggcagtc
    10261 gaaaaatcaa tgtgcttcag cataccctga acaacactga gccggtggag ttgctgggtt
    10321 gtgacgtaga cagccagtgt gcgggtgaag gtttttcctg cctggacagc agctgtctgc
    10381 agggcagcat cgcgatcgcc ggtgctgagc cagaagaccg atgccgtcag ggcaaaactg
    10441 atccctccgg ccgccagact gacaaccgaa ccctcggcaa tatcataagt gaccgattcg
    10501 atggtcccga accgggtgat attacgggcc tgagtataag tcagatgtcc ccgacgaatc
    10561 agccgggatg cttctgcggg atcggttaca ccgggtactt taccttcgcg gatcttattc
    10621 ctcatggttt caacagcgcc ggcatactga tcacggggga cttccagttg catggggcca
    10681 ttatttccca tgtaacgata ctgtccgttt tgtccgtcga atgccgcacc gacgctacgg
    10741 gctgcagttg aacaatattt cgtctggatt tctgttccgc tgaccagtct gtctgctcca
    10801 tttctggcat tattatcacc gaggatccgt gcgtttttca gacggactct gtcgacaata
    10861 tggtttcccc
//
LOCUS       DAZKHC020000126        10859 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZKHC020000126 DAZKHC020000000
VERSION     DAZKHC020000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.126"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..739
                     /locus_tag="ACHOSM_RS20265"
                     /old_locus_tag="pgaptmp_004053"
     CDS             <1..739
                     /locus_tag="ACHOSM_RS20265"
                     /old_locus_tag="pgaptmp_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HGU4560442.1"
                     /translation="LSIVVTIIFFRQAFKLDKTGRNKMFVAFVLMLEAVVFYILYAQM
                     PTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYTHLGNKGKDLSMP
                     MKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLGELFISALGLAMI
                     AALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLETLPVYTNVFGKIG
                     LVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(788..1540)
                     /gene="rsmJ"
                     /locus_tag="ACHOSM_RS20270"
                     /old_locus_tag="pgaptmp_004054"
     CDS             complement(788..1540)
                     /gene="rsmJ"
                     /locus_tag="ACHOSM_RS20270"
                     /old_locus_tag="pgaptmp_004054"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HGU4560443.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(1548..3590)
                     /gene="prlC"
                     /locus_tag="ACHOSM_RS20275"
                     /old_locus_tag="pgaptmp_004055"
     CDS             complement(1548..3590)
                     /gene="prlC"
                     /locus_tag="ACHOSM_RS20275"
                     /old_locus_tag="pgaptmp_004055"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HGU4560444.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            3793..4635
                     /gene="rlmJ"
                     /locus_tag="ACHOSM_RS20280"
                     /old_locus_tag="pgaptmp_004056"
     CDS             3793..4635
                     /gene="rlmJ"
                     /locus_tag="ACHOSM_RS20280"
                     /old_locus_tag="pgaptmp_004056"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HGU4560445.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNHSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            4707..6059
                     /gene="gorA"
                     /locus_tag="ACHOSM_RS20285"
                     /old_locus_tag="pgaptmp_004057"
     CDS             4707..6059
                     /gene="gorA"
                     /locus_tag="ACHOSM_RS20285"
                     /old_locus_tag="pgaptmp_004057"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGU4560446.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LDKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6113..6196)
                     /gene="dinQ"
                     /locus_tag="ACHOSM_RS20290"
                     /old_locus_tag="pgaptmp_004058"
     CDS             complement(6113..6196)
                     /gene="dinQ"
                     /locus_tag="ACHOSM_RS20290"
                     /old_locus_tag="pgaptmp_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HGU4560447.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            6936..7289
                     /gene="arsR"
                     /locus_tag="ACHOSM_RS20295"
                     /old_locus_tag="pgaptmp_004059"
     CDS             6936..7289
                     /gene="arsR"
                     /locus_tag="ACHOSM_RS20295"
                     /old_locus_tag="pgaptmp_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HGU4560448.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSADSKNICS"
     gene            7344..8633
                     /gene="arsB"
                     /locus_tag="ACHOSM_RS20300"
                     /old_locus_tag="pgaptmp_004060"
     CDS             7344..8633
                     /gene="arsB"
                     /locus_tag="ACHOSM_RS20300"
                     /old_locus_tag="pgaptmp_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HGU4560449.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLFFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            8646..9071
                     /gene="arsC"
                     /locus_tag="ACHOSM_RS20305"
                     /old_locus_tag="pgaptmp_004061"
     CDS             8646..9071
                     /gene="arsC"
                     /locus_tag="ACHOSM_RS20305"
                     /old_locus_tag="pgaptmp_004061"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HGU4560450.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDEQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(9205..9501)
                     /locus_tag="ACHOSM_RS20310"
                     /old_locus_tag="pgaptmp_004062"
     CDS             complement(9205..9501)
                     /locus_tag="ACHOSM_RS20310"
                     /old_locus_tag="pgaptmp_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGU4560451.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            9608..10609
                     /locus_tag="ACHOSM_RS20315"
                     /old_locus_tag="pgaptmp_004063"
     CDS             9608..10609
                     /locus_tag="ACHOSM_RS20315"
                     /old_locus_tag="pgaptmp_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGU4560452.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2500 a         2828 c         2784 g         2747 t
ORIGIN      
        1 tctctccatc gtcgtcacca tcatcttctt tcgtcaggca ttcaagctgg ataaaaccgg
       61 gcgcaataaa atgtttgtcg cctttgtcct gatgctcgaa gcggtggtgt tttacattct
      121 ctacgcccag atgccaacat cgctgaactt ctttgccatc aacaacgtgc atcatgaaat
      181 tctcggtttt tccatcaacc cggtcagctt ccaggcgctt aacccgttct gggtggtact
      241 cgccagccca atactggcag gcatttacac gcatctgggt aacaaaggca aagacctctc
      301 gatgccgatg aaatttactc tcggcatgtt tatgtgctca ctgggctttt tgacggcggc
      361 agctgcggga atgtggtttg cggatgcaca agggctgaca tcgccatggt ttatcgtgct
      421 ggtgtactta ttccagagct taggtgaact gtttattagc gcccttggcc tggcgatgat
      481 tgctgccctg gtgccgcagc atttgatggg ctttattctc gggatgtggt tcctgacgca
      541 ggctgccgcg ttcttgctgg gcggctatgt ggcaacattt accgcggtgc cggacaacat
      601 taccgatccg cttgagacgt tgcccgtcta taccaacgtg tttggtaaga ttggtctggt
      661 cacgctgggc gttgcagtag tgatgctgtt gatggtgccg tggctgaaac gcatgattgc
      721 gacgccggaa agccattaat tattcttgca gaaagcaggg tagcgttatc gctaccctgc
      781 tttactttta cacgggcgta cctgcataga tatcaaagcg atgcccttta gtcacaaccg
      841 cgtttggcgt ggcgacattc gctagcggtg gcgcgtagtc cgggcgcttt actaccacgc
      901 gtttggtcgc cagcaagcgt gcaggctcca gcaatccatc ggcatcaaga tccggcccca
      961 ccagcgactg aaagacacgc atctcttttt tcaccagcgc gcttttctgc ttatgcggga
     1021 acatcgggtc gagatagacc acctgcgggc gtggggtaat atcggtcagc gccgtcaggc
     1081 tggaggcgtg aattaactgc aaccgctcct gcaaccagcc gccgatttcc gcatccgcat
     1141 aaccacgcgc caggccgtcg tcgagcaatg cggcaacgac tggattacgc tccagcatcc
     1201 gcacgcggca gccgactgaa gccagcacaa aggcatcgcg ccccagccct gcggtggcat
     1261 ccaccacatc cggcaaataa tcgcctttaa tgcccaccgc tttcgccacc gcctcaccgc
     1321 gaccaccgcc gaatttgcgt cggtgcgcca tcgctccgcc aacaaaatca acaaagatgc
     1381 cgccaagttt tggctcatca cgcttgcgca attccagatg ttccggcgtt aacaccagcg
     1441 ccatcaggtt gtcttcatcg tgctccagcc cccagcgggc cgccagaaca gataaggcac
     1501 cgtctccggt gcctgtttca tcaattaagc agattttcac tgaatgatca gcccttaatg
     1561 ccgtaatgct ccagcatcgc atccagctgc ggttcacgac cacggaagcg tttgaacaga
     1621 tccatcggct cttctgaacc gccacggctc agaatgttgt cgaggaacga ctgcccggtt
     1681 tcacggttga aaatgccctc ttcctcaaag cgcgagaaag catctgccgc cagtacgtca
     1741 gcccacaggt agctgtagta acctgcggca taaccaccgg cgaaaatatg gctgaaagcg
     1801 tgcgggaaac ggccccagga tggagacggt actacggcaa ccagtttctt gatttctgcc
     1861 agagtttcga ggattttcgc tccctgatcc gggcggaact cggcatggag gcggaaatcg
     1921 aacaggccga actccagctg gcgcagaata aacagcgccg cctggtagtt cttcgccgcc
     1981 agcattttat ccagcaactc tttcggcagc ggttcgccgg tttcgtagtg accagagata
     2041 aacgccagcg cctcaggctc ccagcaccag ttttccataa actgactcgg cagttcgacc
     2101 gcatcccacg gcaccccact gatcccggat acaccggcgg tttcgatgcg ggtcagcata
     2161 tgatgcagac catgaccgaa ctcgtggaac agggtgatca cttcgtcatg ggtaaacagc
     2221 gccggtttac catttaccgg gcggttaaag ttacaggtca gatacgcgac cggtttttgc
     2281 agagaaccgt cagctttgcg catctggcct acgcagtcat ccatccacgc cccgccgcgc
     2341 ttgttttcac gggcatacag gtcgaggtag aagctaccgc gcagttcgtt gttctcgtca
     2401 tacagttcga agaaacgtac atccgggtgc cagacatcaa catctttacg ctctttagcg
     2461 gtgatgccgt aaatacgttt caccacttca aacaaaccgt taaccgcttt gttttccggg
     2521 aagtacggac gcagctgttc gtcactgatg ctgtagaggt gctgtttttg tttttcgctg
     2581 tagtacgcga tatcccacgg ctgcaactca tcgacgccaa attcggcttt ggcaaaggcg
     2641 cgcagttgtg ccagctcttt ttcgccttgc ggacgcgcgc gttttgccag atcggttaag
     2701 aaatccagca cctgctgcgg gttttctgcc attttagtgg caagtgattt gaaggcgtag
     2761 ttttcaaagc ccagcagttg cgccagttcg tgacgcagcg cgaggatctc ttccatcacc
     2821 ttgctgttgt cccacttacc ggcgttcggg ccttgatcgg aggcgcgggt gctgtaagcg
     2881 cgatacatct cttcacgcag agcctggttg tcgcagtagg tcattaccgg caggtagctt
     2941 gggatatcca gcgtcagcag gtagccttca agttctttcg cttcagcctg ggcttttgcc
     3001 gcagccagcg cgctttctgg catccccgcc agttccgctt cgtcggtaac gagtttggtc
     3061 cagcccattg tcgcatcgag gacgttgttg ctgtactggt tgcccagctc ggaaagacga
     3121 gtggcaatct caccgtagcg ttgttgtttc tctattggca gaccgatgcc agagagttcg
     3181 aagtcgcgca gtgcgttatc aaccgctttt ttctgagccg tgttcagcgt ggcgtaatga
     3241 tcgccatcgc gcaggtcgcg atacgcttta tacagccctt catgttgccc tacccaggtg
     3301 ctgtattccg acagcagcgg cagggtttgt tcgtacgctt cacgcagttc cgggctattt
     3361 ttcaccgagt tcaggtggct gaccggggag aagatacgcc ccagcacatc gtccacttcc
     3421 gccaacggct ggcagagatt ttcccaggtg tacggtgctc cttgcgctac tacgcgctcc
     3481 acattttcgc ggcagtcgtt cagcgcctta gtcacggctg gaacgacatg ttccgggaga
     3541 attttagaaa acggaggcaa ttcaaaggga gtcagtaacg gattcgtcat tagcgcagtc
     3601 ctggttaaag aggttaagga agcgctcaac aggcgcttta cataatgtgt gtagcatggg
     3661 gttaagtgta gtgaatttca atgagaaacg ttacgctttc gcggcggcgg cctcttttcg
     3721 gtatactgtc ctgataggct tttgtgcgcc ccgaatacgg gccgattttt acttaccgga
     3781 acacctttac ccatgctcag ttatcgccac agctttcacg ctggcaacca cgccgacgtc
     3841 cttaaacata ccgttcagag cctgatcatc gagtcgctga aagagaaaga taaaccgttt
     3901 ctctatctcg acacccacgc aggggccggg cgttatcagt taggtagcga acatgccgag
     3961 cgtaccggcg aatatctcga aggtatcgcc cgtatctggc agcaggacga tttgcccgca
     4021 gaactggagg cgtacatcaa tgtggtaaaa cacttcaacc atagcggcca gttgcgttac
     4081 taccccggtt cgccgttgat tgctcgccag ctactgcgcg aacaagacag cctgcaactg
     4141 accgaactgc acccgagcga ttacccgttg ctgcgttctg aatttcagaa agacagccgt
     4201 gcgcgcgtcg aaaaagccga tggtttccag cagcttaagg ccaaactgcc gccggtttcc
     4261 cgccgtggtt taatccttat cgacccgccg tatgaaatga aaaccgacta tcaggcggtg
     4321 gtcagcggga tagcagaagg ttacaaacgt ttcgccactg gtacttacgc actgtggtat
     4381 ccggtggtgc tgcgtcagca aattaagcgc atgatccacg atctggaagc gaccggtatt
     4441 cgcaaaattc tgcaaattga actggcggta ctgccagaca gcgatcgccg tggcatgacc
     4501 gcttccggca tgattgtgat taacccgccg tggaaactgg aacagcagat gaataacgtg
     4561 ctgccgtggc tgcacagcaa actggttccg gcaggcaccg ggcacgccac cgtaagctgg
     4621 atcgtgccgg agtaattgca gccattgctg gcacctatta cgtctcgcgc tacaatcgcg
     4681 gtaatcaacg ataaggacac tctgtcatga ctaaacacta tgattacatc gccatcggcg
     4741 gcggcagcgg cggtatagcc tccatcaacc gcgcggctat gtacggccag aaatgcgcac
     4801 tgattgaagc caaagagctg ggcggcacct gcgtgaacgt cggctgcgtg ccgaaaaaag
     4861 tgatgtggca cgcggcgcaa atccgtgaag cgatccatat gtacggccct gattatggtt
     4921 tcgataccac catcaacaag ttcaactggg aaacgttgat cgccagccgt accgcctata
     4981 tcgaccgtat tcatacttcc tatgaaaacg tgctcgataa aaataacgtt gatgtaatca
     5041 aaggctttgc ccgcttcgtt gatgccaaaa cgctggaggt aaacggcgaa accatcacgg
     5101 ccgatcatat tctgatcgcc acaggcggtc gtccgagcca cccggatatt ccgggcgtgg
     5161 aatacggtat tgattctgat ggcttcttcg cccttcctgc tttgccagag cgcgtggcgg
     5221 ttgttggcgc gggttacatc gctgttgagc tggcgggcgt gattaacggc ctcggcgcga
     5281 aaacgcatct gtttgtgcgt aaacatgcgc cgctgcgcag cttcgacccg atgatttccg
     5341 aaacgctggt cgaagtgatg aacgccgaag gcccgcagct gcacaccaac gccatcccga
     5401 aagcggtagt gaaaaatgcc gatggtagcc tgacgctgga gctggaagat ggtcgcagtg
     5461 aaacggtgga ttgcctgatt tgggcgattg gtcgcgagcc tgccaatgac aacatcaacc
     5521 tggaagccgc tggcgttaaa actaacgaaa aaggctatat cgtcgtcgat aaatatcaaa
     5581 acaccaatat tgaaggtatt tacgcggtgg gcgataacac gggtgcagtg gaactgacac
     5641 cggtggcagt tgcagcgggt cgccgtctct ctgaacgcct gtttaataac aagccggatg
     5701 agcatctgga ttacagcaac attccgaccg tggtcttcag ccatccgccg attggtactg
     5761 ttggtttaac ggaaccgcag gcgcgcgagc agtacggcga cgatcaggtg aaagtgtata
     5821 aatcctcttt caccgcgatg tataccgccg tcactactca ccgccagccg tgccgcatga
     5881 agctagtgtg cgttgggcca gaagagaaga ttgtcggtat tcacggcatt ggttttggta
     5941 tggacgaaat gttgcagggc ttcgcggtgg cgctgaagat gggtgcaacc aaaaaagact
     6001 tcgacaatac cgtcgccatt cacccaacgg cggcagaaga gttcgtgaca atgcgttaaa
     6061 tgttaaaggg caaagagtgg tgtgctcttt gcccttaatt acgtttccgc tatcagttca
     6121 gaagctgaag cagaaagcgg atcagttcca gcagcgcaat taacgctcca agaacgatga
     6181 ttgctttatc aatcacccgt tttctccatg cgatggagtg agaatgcatc cgcttactca
     6241 tccactgcct gtcacggcgc atgtctcatt gttagataag aactctctca ctccggccag
     6301 agcatcagtt aacggcacca cccgtacttc tgaccaggac tttgaaagcg tttatgcgca
     6361 ttgccagagt gaaaatgcct cagagctaac tggataatca tacagtacat gcaggttata
     6421 aaaccagcac gtccttgcaa tagtttcagt atggtattag cattgatgcg ttagatgatg
     6481 gctatctcac tccagtcaga gccaccaact cagggctgga aagtaaaaaa ccgacgcaaa
     6541 gtcggttttt ttacatccgg attcggacaa ggcttaatat gacgatgacc cagtgaaagt
     6601 atataaatcg tcactgcgat atataccgaa gtgctccctc cgccagctga agaaatcgct
     6661 aattcttgca atgttagcca ctggctaata gtattgagct gttagataag aactctctca
     6721 ctccagccag agccaccaac tcagggctgg aaagtaaaaa accgacgcaa agtcggtttt
     6781 tttacgtcct gattcagacc tcctttcaaa tgaatagcca actcaaaatt cacacctatt
     6841 accttcctct gcacttacac attcgttaag tcatatatgt ttttgactta tccgcttcga
     6901 agagagacac tacctgcaac aatcaggagc gcaatatgtc atttctgtta cccatccaat
     6961 tgttcaaaat tcttgctgat gaaacccgtc tgggcatcgt tttactgctc agcgaactgg
     7021 gagagttatg cgtctgcgat ctctgcactg ctctcgacca gtcgcagccc aagatctccc
     7081 gccacctggc attgctgcgt gaaagcgggc tattgctgga ccgcaagcaa ggtaagtggg
     7141 ttcattaccg cttatcgccg catattccag catgggcggc gaaaataatt gatgaagcct
     7201 ggcgatgtga acaggaaaag attcaggcaa ttgtccgcaa cctggctcga caaaactgtt
     7261 ccgcagacag taagaacatt tgcagttaaa aaatttagct aaacacatat gtattttcgc
     7321 atgtgtttta tccaggaggc attatgttac tggcaggcgc tatctttgtc ctgaccattg
     7381 tattggttat ctggcagccg aaaggtttag gcatcggctg gagtgcaacg ctcggcgcag
     7441 ttctggcgtt agttacgggc gtggtccatc cgggtgatat tccggtggtg tggaatatcg
     7501 tctggaacgc gacggctgcg tttattgccg tcattatcat cagcctgttg ctggatgagt
     7561 ccggcttttt tgaatgggcg gcgctgcacg tttcacgctg gggcaatgga cgcggtcgct
     7621 tgctgtttac ctggattgtc ctgcttggtg ctgccgttgc cgccctgttt gccaatgatg
     7681 gcgcggcgct gattttaacg ccaattgtca tcgccatgct gctggcttta gggttcagta
     7741 aaggcactac gctggcgttc gtgatggcgg ccggattcat tgccgatacc gccagcctgc
     7801 cgcttattgt ctccaacctg gtgaatatcg tttccgcaga tttctttggc ctcggctttc
     7861 gcgaatatgc ctcggtgatg gtgccggtgg atatcgccgc gattgttgcc acgctggtga
     7921 tgctgcatct cttttttcgc aaagatattc cgcagaacta cgacatggcg ctgctgaaat
     7981 ctcccgcaga agcgattaaa gatcctgcta cgttcaaaac tggctgggtt gttttactgc
     8041 ttctgctggt gggatttttc gtcctcgaac cgctcggcat tccggtgagc gccattgcag
     8101 ctgtgggcgc actgatatta tttgtcgtcg ctaaacgcgg tcatgcgatt aatacgggta
     8161 aagtcctgcg cggtgcaccc tggcagattg tcatcttctc gctcggcatg tatctggtgg
     8221 tttatggcct gcgcaatgcc ggattaacgg aatatctttc tggcgtactc aacgtgctgg
     8281 cggataacgg cctgtgggcc gcgacgctcg gcaccggatt cctcaccgcc ttcctctctt
     8341 ctattatgaa caatatgccg acggtactgg ttggcgcgtt gtccattgat ggcagcacgg
     8401 cgtctggcgt tatcaaagaa gcgatggttt atgccaacgt gattggctgc gatttggggc
     8461 cgaaaatcac cccgattggt agtcttgcga cgctgctctg gctgcacgta ctttcgcaga
     8521 agaatatgac tatcagctgg ggatattact tccgtacagg gattatcatg accctgcctg
     8581 tgctgtttgt gacgctggct gcgctggcgc tacgtctctc tttcactttg taatgagata
     8641 ttgatatgag caacattacc atttatcaca acccggcctg cggcacgtcg cgtaatacgc
     8701 tggagatgat ccgcaacagc ggcacagaac cgaccattat ccattatctg gaaactccgc
     8761 caacgcgcga tgaactggtc aaactcattg ccgatatggg gatttccgta cgcgcgctgc
     8821 tgcgtaaaaa cgtcgaaccg tatgaggagc tgggccttgc ggaagataaa tttactgacg
     8881 atcggttaat cgactttatg cttcagcacc cgattctgat taatcgcccg attgtggtga
     8941 cgccgctggg aactcgcctg tgccgccctt cagaagtggt gctggaaatt ctgccagatg
     9001 agcaaaaagg cgcattctcc aaggaagatg gcgagaaagt ggttgatgaa gcgggtaagc
     9061 gcctgaaata aagcggcgat atccactgcc gatagttggt aaagtgcgct ttgtttatgt
     9121 cggatgcgac gctaacgcgt cttatccgac ctacaaaacc gtgcaaattc aatatatggc
     9181 atgaaatatg tagagcgtag cgcatcaggc aattttgcta tttacgcagc actcctcgcg
     9241 taagaattca attatcgctt ccagcattgc gtattgcgaa acacacatca gcgtccgact
     9301 ctcacggttt tgtttgacca gtcctaccga taccagtgct gatatatggt ggctgagtgt
     9361 ggagccaggt atcccaagct gcttttgcaa ctcaccgacc ggcagccctt gttccccggc
     9421 tttgacaaga tgtttaaata tgaacaaacg ggtcgggtga cctagctctt tcagcgcttt
     9481 agcgacttct tccaactgca tatgacaccc ctctgtctgg ttgatatttc gataatacca
     9541 gaaatatctt gatctgaatt tcatttcgat tattctggaa atatagattt atccaatgag
     9601 gttacgtatg agtagttggc ttgcgatgct gcaagatgcc gcagagatgt ttgtgtttct
     9661 cgccgtcgag ctttcgttgc tgtttatagt gattagtgcc ggtgtcagcc tgataagaca
     9721 aaaggtgcca gaccataaaa tccagcagat gatgggggcc agaaaaggga gaggctatct
     9781 cctggctgct ctgttgggag ccgttacccc gttctgtagt tgctcgacaa tacccatgtt
     9841 acgtggattg ttgtcagcga aagccgggtt tggtccgact ctcacttttt tatttgtttc
     9901 accattactt aatcccatta tcgtcggact aatgtggatg acctttggtt ggaaagtcac
     9961 cttgttgtac gcgattatcg cagccggcgt ctccgttctt gccagtatta tcctggattc
    10021 tctggggttt gaacgtcata tcattgccag taaaagctca tcagcaaatt gttgtgctcc
    10081 agcaaaaact tcgccgggaa cgacatatac gcccataaaa gtgagttgtt gtagtccagc
    10141 ggctaaagcc attgagaacc ccgtagttac ctgttgcagt accaaagctg tagtaagtat
    10201 taatcccata aaactagcca ccaaagatgc gttgcaacag tttaaagatg tactgccata
    10261 tcttttgtta ggagtattaa taggatcttt tatttatggc tttattccct cagcgtggat
    10321 tgccgctcat gcaggagcag ataatccctt cgccatccca ttaagcgccg ttgtcggtat
    10381 tccgctgtat atccgggctg aggcagttat ccctctggca tctgttctga tgacaaaagg
    10441 aatgggtctg ggagcattaa tggcattaat catcggcagt gccggcgcaa gcctgacgga
    10501 agtgatatta cttaaatcaa tgttcagaat gccgatgata gctgcattcc tgacggttat
    10561 attaggtatg gctatcttga tgggctattt gactcaattg ctattttaaa tagatgccag
    10621 cagtaaacta taagatgcat agttagtgcc atatccttct aagtagaaaa tcatagacag
    10681 gataatcctt aaaaatcaca aatgatttaa ctaatgcatt gagaatgatg acggctcggt
    10741 caacaagtca tcaacagaca ataaccacgt aatcatgctt ctctgacatt ttatcggaga
    10801 agcaatgact ctttgtctca gtaccctcct cctttttaaa aggcagattt cagtcgcta
//
LOCUS       DAZKHC020000127        10338 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZKHC020000127 DAZKHC020000000
VERSION     DAZKHC020000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.127"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            50..469
                     /locus_tag="ACHOSM_RS20320"
                     /old_locus_tag="pgaptmp_004064"
     CDS             50..469
                     /locus_tag="ACHOSM_RS20320"
                     /old_locus_tag="pgaptmp_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HGU4560453.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            466..1026
                     /locus_tag="ACHOSM_RS20325"
                     /old_locus_tag="pgaptmp_004065"
     CDS             466..1026
                     /locus_tag="ACHOSM_RS20325"
                     /old_locus_tag="pgaptmp_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HGU4560454.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            1027..1272
                     /locus_tag="ACHOSM_RS20330"
                     /old_locus_tag="pgaptmp_004066"
     CDS             1027..1272
                     /locus_tag="ACHOSM_RS20330"
                     /old_locus_tag="pgaptmp_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HGU4560455.1"
                     /translation="MKTILIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            1269..2768
                     /locus_tag="ACHOSM_RS20335"
                     /old_locus_tag="pgaptmp_004067"
     CDS             1269..2768
                     /locus_tag="ACHOSM_RS20335"
                     /old_locus_tag="pgaptmp_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HGU4560456.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATEFWGRGSMIALMVAEFIAINPDAELYAIAQGSGT
                     GQAQACLMNIIGTASQDGVLHIYIGGRRYLLAVTKGQKGKELIDALVSVINADADAPF
                     SAYAGQVADNASGLKGSMGINARFTGECSVHDIRVNYHDGETTPGGLTIELGYPKTKA
                     TNPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWMAHTGT
                     FGEVQAFGAKRNDFLATCTFIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALPS
                     RMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLDV
                     ETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWVE
                     LGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            2777..3142
                     /locus_tag="ACHOSM_RS20340"
                     /old_locus_tag="pgaptmp_004068"
     CDS             2777..3142
                     /locus_tag="ACHOSM_RS20340"
                     /old_locus_tag="pgaptmp_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGU4560457.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASATSTRVQ"
     gene            3157..3633
                     /locus_tag="ACHOSM_RS20345"
                     /old_locus_tag="pgaptmp_004069"
     CDS             3157..3633
                     /locus_tag="ACHOSM_RS20345"
                     /old_locus_tag="pgaptmp_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HGU4560458.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            3760..5835
                     /locus_tag="ACHOSM_RS20350"
                     /old_locus_tag="pgaptmp_004070"
     CDS             3760..5835
                     /locus_tag="ACHOSM_RS20350"
                     /old_locus_tag="pgaptmp_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4560459.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     PSPVPPSPSSGGAGFWGRMMGRAGGLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGAL
                     WAGAMAAPVLLDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNY
                     LGGWLTEAWQKLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNV
                     WNGGNYGLW"
     gene            5822..7171
                     /locus_tag="ACHOSM_RS20355"
                     /old_locus_tag="pgaptmp_004071"
     CDS             5822..7171
                     /locus_tag="ACHOSM_RS20355"
                     /old_locus_tag="pgaptmp_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HGU4560460.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGQVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            7155..8279
                     /locus_tag="ACHOSM_RS20360"
                     /old_locus_tag="pgaptmp_004072"
     CDS             7155..8279
                     /locus_tag="ACHOSM_RS20360"
                     /old_locus_tag="pgaptmp_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HGU4560461.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSAR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            8269..8883
                     /locus_tag="ACHOSM_RS20365"
                     /old_locus_tag="pgaptmp_004073"
     CDS             8269..8883
                     /locus_tag="ACHOSM_RS20365"
                     /old_locus_tag="pgaptmp_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGU4560462.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNVTVDKNLHVKGNV
                     SIDGTGRSQGTFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            8876..9313
                     /locus_tag="ACHOSM_RS20370"
                     /old_locus_tag="pgaptmp_004074"
     CDS             8876..9313
                     /locus_tag="ACHOSM_RS20370"
                     /old_locus_tag="pgaptmp_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HGU4560463.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPIGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHDRLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            9313..>10338
                     /locus_tag="ACHOSM_RS20375"
                     /old_locus_tag="pgaptmp_004075"
     CDS             9313..>10338
                     /locus_tag="ACHOSM_RS20375"
                     /old_locus_tag="pgaptmp_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGU4560464.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLK"
BASE COUNT         2235 a         2679 c         3228 g         2196 t
ORIGIN      
        1 aggatgctcc ccggaaaaag accggtaaca aagcgaaaca ggcccgtgca tgaattacgc
       61 cactgaaacc gatatgcggg cgcgttaccg cgaggatttg ctcaggccgt tactggctgt
      121 gccccgttcg gatgagccgg atacgcgcaa gctgaaccgg gcgctgacag atgcgtcagc
      181 ccttatcgac agctatctgt ccgcccgtta cacgctgccg ctggaggtta ttccggccgt
      241 tcttgttcag cactgttgcg cgattgcctt ttattacctg tgcgatcagc gagcctcaga
      301 tcaggcgcgt gaccgttacc gtgaggcgct ggcctggctg aaagatgtca tgaacggcaa
      361 tgtgccggta ggtgtggata cgaacggagc ggcccctgaa tccggggatt taccacaggt
      421 tcagtctgat gcagcggtat tcgggcgcaa ccagaagggc ttcatatgat tacggaaacc
      481 gaacaggcat acatcgcccg tatccgtgag tatttcggga atgaactggt gtctgttgac
      541 acgcatcccg gcgactggag cgacagcgta ctgcgcacca tgctgattaa cgccccggcg
      601 atctacgttg cctggctggg tgccggtgaa ggccgtaccc gtggtcgcct ggtcagtcac
      661 tgggtgttct acgtcatcgg cgacatgctc aacgggcgtg aggccagccg tcccggattg
      721 tatcagattg tggcccggct gattgccgtg cttaacggct tcagaacaga aaaaacctca
      781 ccgctttact ttgaaaaggc ggtcaacggt tacaccgaaa cccaggcgga cagtggtgcg
      841 gtgatgtatg cgctttattt ctcctgcgag gaaatgatcg caccgctgac cgatatcagc
      901 tcgctggacg acttcctgcg tcattacgaa acctttgtgg agccgcccgg tacgccgccg
      961 tttgaggcac atattgattt gccggggaac acgacaacgg cggtcgaacc gccggaggaa
     1021 ctgtaaatga aaaccatctt aatcaaaccc gcgccgggac gcctgattcg tgatcccgac
     1081 acaatgcgcc cgctggcaca ggagggggag gaaaagccct ttacgccgtt ctggtgtcgg
     1141 cgtcttgatg acggcgatgt cattcaggct gaaaaagccg ctgaagaggc tccggctgta
     1201 tctgcggatg caacgactgc gacagaaaaa ccggtcgccc aaccggcatc cgataaggag
     1261 aaaccgcaat gattaatttt gacgggatcg gtaatgaccg ccgtataccg ctgattgagg
     1321 ttgagtttaa taactccatg gctgtcaccg gcactccggc acagcgtcag tgtgtgctgc
     1381 tgtttggtca ggcgagaatg aaagagaatg ccgtggacgg tgccggtgtt ctggatgttc
     1441 cggtcaggat cacccgtgcg tctcaggcaa cagaattctg ggggcgcggc tccatgattg
     1501 ccctgatggt ggcggaattt atcgccatca atcctgatgc tgaactgtat gccattgctc
     1561 aggggagcgg caccggtcag gctcaggcat gtctcatgaa tataattggc accgcctctc
     1621 aggatggtgt tctgcacatt tatatcggcg gtcggcgtta tctgctggct gtcacgaagg
     1681 ggcagaaagg taaggagctt attgacgctc tggtctctgt tattaatgcc gatgctgatg
     1741 cgcccttcag tgcttatgca ggtcaggtag cagataacgc cagcggactc aagggaagta
     1801 tgggaattaa tgcccgtttt accggtgaat gttccgtaca tgatatccgt gttaattatc
     1861 atgacggaga aaccacaccg ggtggactga ccattgaact tgggtatccc aaaacaaaag
     1921 ccactaaccc ggatatcacc cgaagcgtgg cgggtatggg cgatcgccag tacaactatg
     1981 tcgtcatgcc ctataaggat gatgcaaacc tgaaaatcat cagtgatgag cttctgaaac
     2041 gctggggacc ggccaaaatg tctgacggtg tgctgtggat ggcgcatacc ggcacgtttg
     2101 gtgaggtgca ggcattcggg gcaaaacgca atgattttct ggcgacctgc acattcattc
     2161 ccaaagcccc tgaaccggat tacctctggg cagcggcggt ctgcgccacc tgtgcgccgt
     2221 ctctgggggc agatcctgcg cgtccgcttc agacgctggc gcttccgtca cgcatggccc
     2281 cggccccggc cgatcgtctg acgcgtgaag agcgtaacag tctgctgtgg ggagggattg
     2341 ccacggtcag tgtggctgcc ggtgatgtgg ttcagatcga acgtcaggtg accatgtatc
     2401 gccagaacgt gtacggcgaa agtgatccca gctatctgga tgtggaaacc atctacaccc
     2461 tctcctggct gcgttattcc ctgcgtactt ttatcacaca acgttttccg cgccataaac
     2521 tggctgatga cgggacgccc gttcgtgccg gtcagaatat cgtgacgccg gagatcatga
     2581 agcttcagtt aatttcgctg ggtgaagagt gggtggaact cgggctggtc gaaaatctgg
     2641 acacctttaa aaagaatctg cttgtggagc gtaatgcgtc agatcgaaac cgtcttgacg
     2701 tgttatgcac gccggatctg gttaaccagt tccgcttcct ggcagcacag atccgtttca
     2761 ttttgtgagg taaggcatga gcggaaaaca gtatcagggc acggccacta tccgtgtgaa
     2821 cggtcaggaa tacgagacgc ttgagggggc tacattttct ccgtcaggtt ttgaacgtga
     2881 agtggtgaaa ggcgcgaaag tctatggtta ccgccagaaa ccacgtgaag cgacactgga
     2941 ctgcaaattt ccggcaggcg gagaaggttc accagccgcc gatgaaatca acaactggac
     3001 tgctgtcact atcgagtttg tggcggatac cggcgaagtc cacatgatga cgaaggcctg
     3061 gagcagtgaa ccggcctcgc ttgacggtgg cggggagatc tccgtgaagt tcgccagcgc
     3121 caccagtacc cgtgttcagt gatcaggaaa aataaaatga ccacacgtaa gaaaaaaacg
     3181 gcagtttccg aggcagccgt gatggaggca atccgggaag cacttgaggg ggctgatcca
     3241 cgcactgccg ggctgacaga gcaactggca aagggatatg tggatctgct tgatggtctg
     3301 ccgtttggtg agacccgtga atatcgtgtc acgttccggg aactgacagc gaaagacagt
     3361 attgacgcag aagcagaagc cgagcgcgtg gtggagacaa acaatggccc gatgctgata
     3421 gcgtctccgt cgttacgcgg tgttgcactg ctgcgccgtc agattgccgc agtgggagac
     3481 attgaaggtc cgttgtcacc ccgtcagatt ggacagttaa gcgagcgcga tctctcccgc
     3541 ctgatggcag cggtcagcct gctggatacc gcgcttgccg gaaagctggc agctgaccgg
     3601 gggcgatcag gcgcagtgtc gggatcagat tgaagaagcg gcaattatcc tggggatggt
     3661 gacaaaaagc ggtccggaat gggcgcttaa cctccccctg tcgcaacttt accggcactg
     3721 ccgacagaca gaaaaaatca tcagaacgaa gcagtaagta tggcccgaaa tctcagagca
     3781 tctctgatag ttgatttgct cggcaatatc tcagccaaat cccgccagtg gtcacaggag
     3841 ctgggggcat tctcacgctc cggccaggcg gggctgggtg gcctgggaaa tgctgcccgc
     3901 cgtgccgggc aggaaacaga tgtactcgga agcaggatgc agcgcacgct ggccggggtg
     3961 cggggcagta tccgccatgt cacttctgat tttgaccgtc ttcagggcag tattaccggc
     4021 accatcgggc ggatcagcaa tctttacgga atgctggccg gtggtgctgc tgtatatggc
     4081 tttaaccggg gatttattcg cccggcagcg gagatggaaa cctatatgat tcgcctgaac
     4141 tccctttaca aaggggatcg ggcgaaaact gatgatgtcc gcaggtgggc aatacagaat
     4201 gcgaaggaaa ccacctgggg gctggctggc gtcatgcagg aatatacgtc cagtctgggc
     4261 tttggtatga gtgacaggga ggcccgaaat tttgtcacca tgcttcagga tcagggtgcc
     4321 gtgggcggct ggtcactgtc cgatgcacag ggggcatccc tgcaactgaa acagatgtat
     4381 gcaagaggca gtattcaggc ggcggatgca aatattcttg ccacctacgg cattaatgcc
     4441 taccgcgtgc tggctgatcg tctgggtgtg gaccagaaag tggtcagaaa acttggtgaa
     4501 aaagggctgc ttgggccgga cagtattcgc ctgttatttc agacgctggc ggaacaggcc
     4561 aggggagcac aaaaggacgc aatgaattcc tggagtggcc tgacggccat gatgggtgac
     4621 gtctgggatc agtttgcccg ggatgtgatg gacagtgggc catttgaaaa actgaagggt
     4681 aatctcaggg gatttctttc atgggttgac agtgccaaat ctgatggcag ttaccattcc
     4741 ctggcggaaa gcacggcatc agccatgaac cagggctttg agtatgcccg tgatgcagtg
     4801 acagggtttt atcaggcgat cagaaaggta cgcgacacgc ttcaggctct gcgggatgcg
     4861 ggctatggtg atgcgctgga tcgcattggt cagggggcac agaccgccgc gaaatacctg
     4921 ctgtacatgt atctcgccac ccgtgccctg aaaatggcgc gggctgtcgg aacggggatt
     4981 atacgtccgg gtgctgcatt gctgggatac ggattatcag cggcggcctt cctgacctca
     5041 cctttccggc gctctcctcc ggccggaaca ccaccaggca catctcaggg acgagggcaa
     5101 cgttttataa actttcttac cggggtaaat ccggcagccg ttcagccagt acttgtaaca
     5161 aactggccca caggaggact gtccggcacc ggcgcgaccg atacatcctc aggcggacgt
     5221 caggggcggg gccgcaggaa acgcggaccg gggcgcggaa gaccggtcac gcctcccctg
     5281 ccaccgtccc cagtgccacc gtctccctct tctggtgggg cagggttctg ggggcgcatg
     5341 atggggcgcg ccggtgggtt actttcgtct gtcggtagcc gcatggggct ggggcgtttc
     5401 tccggctttt tccgcagtgc cggtggactg gccggccggc tgggtggagg tgccctgtgg
     5461 gctggcgcga tggcagcccc ggttctgctg gatggcagcg ccagtgccac agacaaggga
     5521 gaagcggtcg gctctcttgc aggcagtatt gccggtggtg cgctgggggc ggcagccgga
     5581 cctgtgggga ttgccattgg ttctacggtg ggcagctatc tcggtaacta tctgggggga
     5641 tggctgactg aagcctggca gaaattacgg ggcagtgatg atgaaagcag tggacaggcg
     5701 gtacaaaaag cctctgcccg tgtggagctg gttgccccgg aagggtggca ggctcgcagt
     5761 attgatattg atgacacgtc cggtcacgga ctggatgtga atgtctggaa cggagggaat
     5821 tatggcctct ggtgacggtc gtggtgcgtt ccgtggtgtg ccgttcctcg tctggcgtga
     5881 gcagcgcgaa cgtggtgggc gaaacattgt ccgtcgggaa tatccgttac gggaaaccgg
     5941 tggtgcagac gatctgggac ctaaactggc tgaatttacg ttcagtgtac ttgtgatggg
     6001 ggatgatgtt cagacacagc gaaatcgtct gcgtgatgcc cttcgtgctc ccggtgccgg
     6061 ggagctgctt cacccggatt acggcacgtt aaatgtgctg ataaacagct ttgaaagtcg
     6121 ttataacgcc gctgaacagg gcgtggttga gttcaccatt aatgtcacgc cggtaagtga
     6181 tgataccgca cctgctgtca cacaggacac ggccgccatt ctggagcaga agagtaccac
     6241 cgcgctcggg caggtttttg aaaccctgga agcgggctgg acggtgatct ctgacggaat
     6301 gcacgatgtc caggcaatga ccgaaaccat cagtgataag gtgtccgccc tggaaaatgc
     6361 cgtttccggc atggggattg tacaggatat cagtgccttt actgccacgt tcacggcgct
     6421 gaaagggaat gccacggcat tactgacagc accttcccgc atggcctcat cttttgcggg
     6481 gcttttcagt gccctgatca ccctgccatc gctgccgtca ctgtcttcag gaggcctgaa
     6541 tacccggccc ggtggcagta tcggccggac gacatccgcc gtctctcagg gaatgccgca
     6601 actgtacagg acattatctt ccctgcgtta cgtgctggat gagcaggata atccgcagac
     6661 tctcatcggt ctgacaccgg cagcacaaaa gaatattcgt ctgatacggg cggtaatgca
     6721 gagtgctgcc gtggtggccc aggcacagac cgtgggaaaa ctgctggatc aggttctcag
     6781 tcaggaaacc ctgccggaca gtgacgcagc ccaccgtacc tggcctgtct ggctggaaag
     6841 ttcagttgat cttcagcgca ttaaccgtga cttaagcgaa gcgctcgaac ggcaggtgat
     6901 gacactgtcc gggcaggggt ataccgccac ggcgctgacg cttcgtgatg ccagactggc
     6961 actgacagag gatctgaata cacggggagt tcagctaccc ggtgcaacag tagtgactgt
     7021 acgtaccacc gagcctgcac tggtgaccct gtaccgtgcc accgggaaca gtaccggctg
     7081 gcaacgtttt gtgcgccgta acggtattgt tgatccgctg ttcattcccg gaggccattc
     7141 agtggaggtg attagtgagc agcagggttg aactgtatct gggcggtgag attttttccg
     7201 gctggctgac ggtgagtgtt cgtcgctctc ttgaacatct ggcgggctcc tttgaactgg
     7261 gggtaatgat gcccggtgta cgccttccgt catccgtccg tgccggtcag tctctggaat
     7321 tgcgcattga cggtcagcct gtgatcactg gctggctgga tcaggtccgg cagcgcatca
     7381 gcgccacgcg ttttcagatc acgctcagcg gacgggataa aaccggtgac ctggtggact
     7441 gttcagccat tcatccgggc agccagtgga ggaaccgcac gctggagcac attgcttcag
     7501 atttgtgtgc tccgttcggg gtcacggtgc gctggcaggt aaatgatgca acggcagccc
     7561 ggcccttttc caccttcaca ctggaaaact cagaaaccgt ggcagatgcg ctgacacggg
     7621 ccgcgagaca cagaggcgtc ctggtaacca gtaatgccgc cggtgaactg gttttcactc
     7681 aggccggcag tcagcgtggc gacacgctga cgctgggcga aaatctgctg gatttggatc
     7741 acaacgtgga tcaccgtctg cgccacagtg aataccgtgt acgggggcac gggcgtggtg
     7801 gtggtcatgc cggggatgca ctgacagccg gaacgctggc cgcacccgtt ggtacggtga
     7861 cagacagtgc catccaccgt tacagaccga aaattgtgct ggcggatcat gctgttgatg
     7921 cagacggtgc acgccagagg gctgtccggg aaatgcgccg ggcggttgcc cgctctgcgc
     7981 gcctgacagc caccgtgcgg cactggtttc gggagaacgg ccagttgtgg gatatcaacc
     8041 tgctgacggc tgtcacggct ccccgcaccg gagtggaaga gcgtgatctt cttgtctgtc
     8101 aggtggagtt ttcgctggat gcaaatcacg gcgaaaccac ccgtctgatt ctggcacccc
     8161 gtgacggctt tattgttccg gcagagccgg gaaacagcgg aagcggaaat gcgggtgacg
     8221 tggacgcctt cgtgcgggca cagatgaaaa aacaggggat taaattcaat gatgaatgac
     8281 gaagtcatca gccgccttct ggcccccgtg atgcggggtg ttcgtctgct gttcgggcgt
     8341 ggtgtactga ccggcacaac ggacacgctg aaaatccaga atgtgcaaat caccggtatg
     8401 gatggtgaaa cctttgatga cgttgaacgc ccccagcagt acgggcaaat cagcgtcccc
     8461 ctgcctggtg cggaagtttt tctggcctgt gctggcggac aacgggatca ggccgtggtg
     8521 cttgtggtgg aagaccgccg cagtcgcccg accggactta ccgccggaga taccggcgtg
     8581 tatcaccatg aggggcaccg tatacgtctg acaaagaacg gccggatcat tgtgacctgt
     8641 aagacgctgg agatttacgc cgatgaaggt gttcaggtgg atacaccgga ggctcacttt
     8701 accggtaatg tcacagtgga taagaacctg catgtcaaag gcaatgtgtc cattgacggc
     8761 accggaagat cacaggggac gttcacgatg tccgaagcgg ttattgccgg gatcacctat
     8821 tcaggtcatg tgcatcacga taacggtgaa ggcagcaaaa cgggaggacc agagaatggc
     8881 tgatattgct gtcgtctggg atcagggttg tggttccctg caactgaacg gcgcagatct
     8941 tctgacggat aacagcctgc tgactgcggt cattatttca ctgtttacgg acaggcgggc
     9001 gctggattcc gatgaaatcc ctgacggcac ccgtgaccgt cggggatggt ggggagacag
     9061 tttccgggag cgccccattg gctcccgtct ctggctgtta agccgtgaaa agacgctgtc
     9121 ctccgtggtc agccgtgcac aggcctatgc tgatgaagcg ctggcgtggc tgcataaaag
     9181 cggtgctgcc acatccgtgg tatgtcatgc catgcgtgtg gggcatgatc gcctttcgct
     9241 ttccgtgaaa atcaccctgc cggacggaag cagacatccg atgatttttt atgctgatat
     9301 gaagggggaa tgatgcctta tcagccctta ccactggcgc aactgatcac acagacacaa
     9361 caggatatca gccagcgcct gcccggttcg cagccgggcg tgaatgaaac caccctgaat
     9421 gccattgcgt atgcactggc gggtctgtca gcacaggagc atgaacatct ggcctggatc
     9481 tcccggcaga taattccgac agaagctgat gaagccgaac tcctgaaaca ctgcgcattc
     9541 tggggtgtca tccgtaaacc ggcttcccgc gctgacggac cggtacaact gatgctgacc
     9601 acggatgcag ggatcacgga aggcgtactc cttcagcgaa gcgatggtgt tgtgtaccgc
     9661 atcaccggct ctgcgaccgg aaaagccgga acactgaatg ttaatgtgga ggcggaaagt
     9721 gcggggcgcg ccggaaatac cccgaccgga acccgcctgt cctttatcac gccacaggcg
     9781 ggcatcaacc agacagccac ggttaccggc acgggactca ccggtggtgc ggatgtggaa
     9841 acggtgccgg agttgctgtc caggctggta ttccgggtac agaacccgcc atcaggggga
     9901 acacagtatg attttgaacg ctgggcacgg gaagtaccgg gcgtgacgcg ggcatggtgt
     9961 aagcctgaat ggcctgaggc gggtagtgtt ggtgtgactt ttgttcagga taataaccct
    10021 gacattttcc ccggagaagg tgatgtgaag cgggtggcgg attatatccg cagtcatgat
    10081 gatccggcga cgggccagcc cgttggtcag ccacttgggc cgacaatcag cgtgtttaag
    10141 ctgaccaata agccggtggc gtttgagatc aggattgtac ccaaaacgcc ggaaaatcag
    10201 gctgccgtaa aacaggcatt aacggacctg ctttataacg aatcgcggcc gggtggactt
    10261 gtattgcctt catcattctg gcgggctgtt gcaggggtga aaggactgga ggattttgaa
    10321 gttcgcagtc cgctgaag
//
LOCUS       DAZKHC020000128        10333 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZKHC020000128 DAZKHC020000000
VERSION     DAZKHC020000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.128"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(119..244)
                     /locus_tag="ACHOSM_RS20380"
                     /old_locus_tag="pgaptmp_004076"
     ncRNA           complement(119..244)
                     /ncRNA_class="other"
                     /locus_tag="ACHOSM_RS20380"
                     /old_locus_tag="pgaptmp_004076"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(275..350)
                     /locus_tag="ACHOSM_RS20385"
                     /old_locus_tag="pgaptmp_004077"
     tRNA            complement(275..350)
                     /locus_tag="ACHOSM_RS20385"
                     /old_locus_tag="pgaptmp_004077"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(315..317),aa:Lys,seq:ttt)
     gene            complement(384..459)
                     /locus_tag="ACHOSM_RS20390"
                     /old_locus_tag="pgaptmp_004078"
     tRNA            complement(384..459)
                     /locus_tag="ACHOSM_RS20390"
                     /old_locus_tag="pgaptmp_004078"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Lys,seq:ttt)
     gene            complement(606..681)
                     /locus_tag="ACHOSM_RS20395"
                     /old_locus_tag="pgaptmp_004079"
     tRNA            complement(606..681)
                     /locus_tag="ACHOSM_RS20395"
                     /old_locus_tag="pgaptmp_004079"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(646..648),aa:Lys,seq:ttt)
     gene            complement(685..760)
                     /locus_tag="ACHOSM_RS20400"
                     /old_locus_tag="pgaptmp_004080"
     tRNA            complement(685..760)
                     /locus_tag="ACHOSM_RS20400"
                     /old_locus_tag="pgaptmp_004080"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(725..727),aa:Val,seq:tac)
     gene            complement(910..985)
                     /locus_tag="ACHOSM_RS20405"
                     /old_locus_tag="pgaptmp_004081"
     tRNA            complement(910..985)
                     /locus_tag="ACHOSM_RS20405"
                     /old_locus_tag="pgaptmp_004081"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(950..952),aa:Lys,seq:ttt)
     gene            complement(1150..1941)
                     /gene="cpoB"
                     /locus_tag="ACHOSM_RS20410"
                     /old_locus_tag="pgaptmp_004082"
     CDS             complement(1150..1941)
                     /gene="cpoB"
                     /locus_tag="ACHOSM_RS20410"
                     /old_locus_tag="pgaptmp_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HGU4560465.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1951..2472)
                     /gene="pal"
                     /locus_tag="ACHOSM_RS20415"
                     /old_locus_tag="pgaptmp_004083"
     CDS             complement(1951..2472)
                     /gene="pal"
                     /locus_tag="ACHOSM_RS20415"
                     /old_locus_tag="pgaptmp_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HGU4560466.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2507..3799)
                     /gene="tolB"
                     /locus_tag="ACHOSM_RS20420"
                     /old_locus_tag="pgaptmp_004084"
     CDS             complement(2507..3799)
                     /gene="tolB"
                     /locus_tag="ACHOSM_RS20420"
                     /old_locus_tag="pgaptmp_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HGU4560467.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3932..5197)
                     /gene="tolA"
                     /locus_tag="ACHOSM_RS20425"
                     /old_locus_tag="pgaptmp_004085"
     CDS             complement(3932..5197)
                     /gene="tolA"
                     /locus_tag="ACHOSM_RS20425"
                     /old_locus_tag="pgaptmp_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HGU4560468.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAGADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(5262..5690)
                     /gene="tolR"
                     /locus_tag="ACHOSM_RS20430"
                     /old_locus_tag="pgaptmp_004086"
     CDS             complement(5262..5690)
                     /gene="tolR"
                     /locus_tag="ACHOSM_RS20430"
                     /old_locus_tag="pgaptmp_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HGU4560469.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5694..6386)
                     /gene="tolQ"
                     /locus_tag="ACHOSM_RS20435"
                     /old_locus_tag="pgaptmp_004087"
     CDS             complement(5694..6386)
                     /gene="tolQ"
                     /locus_tag="ACHOSM_RS20435"
                     /old_locus_tag="pgaptmp_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HGU4560470.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(6383..6787)
                     /gene="ybgC"
                     /locus_tag="ACHOSM_RS20440"
                     /old_locus_tag="pgaptmp_004088"
     CDS             complement(6383..6787)
                     /gene="ybgC"
                     /locus_tag="ACHOSM_RS20440"
                     /old_locus_tag="pgaptmp_004088"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU4560471.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6937..7230)
                     /gene="ybgE"
                     /locus_tag="ACHOSM_RS20445"
                     /old_locus_tag="pgaptmp_004089"
     CDS             complement(6937..7230)
                     /gene="ybgE"
                     /locus_tag="ACHOSM_RS20445"
                     /old_locus_tag="pgaptmp_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HGU4560472.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(7230..7343)
                     /gene="cydX"
                     /locus_tag="ACHOSM_RS20450"
                     /old_locus_tag="pgaptmp_004090"
     CDS             complement(7230..7343)
                     /gene="cydX"
                     /locus_tag="ACHOSM_RS20450"
                     /old_locus_tag="pgaptmp_004090"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HGU4560473.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(7358..8497)
                     /gene="cydB"
                     /locus_tag="ACHOSM_RS20455"
                     /old_locus_tag="pgaptmp_004091"
     CDS             complement(7358..8497)
                     /gene="cydB"
                     /locus_tag="ACHOSM_RS20455"
                     /old_locus_tag="pgaptmp_004091"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU4560474.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8513..10081)
                     /gene="cydA"
                     /locus_tag="ACHOSM_RS20460"
                     /old_locus_tag="pgaptmp_004092"
     CDS             complement(8513..10081)
                     /gene="cydA"
                     /locus_tag="ACHOSM_RS20460"
                     /old_locus_tag="pgaptmp_004092"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HGU4560475.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2526 a         2802 c         2481 g         2524 t
ORIGIN      
        1 taattccacc gacgtagagt tgcttgcaca gtaagctctg acgatgtcat caggtttaca
       61 aagtgaaatt gaggatgcct gaatagcaaa aaagcgcctt tagggcgctt ttttacattg
      121 gtgggtcgtg caggatgact cgcttcgctc gcccttcggg ccgtcgccgc gagcggctcc
      181 gttgtttcgc tgcgctcaac tcaaacctgc tgcaggttcg aatctttgag tatcgcagat
      241 agcaaaaaag cgcctttagg gcgctttttt acattggtgg gtcgtgcagg attcgaacct
      301 gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc cttgcgggat
      361 tatgctgctt taatcattca ggatggtggg tcgtgcagga ttcgaacctg cgaccaattg
      421 attaaaagtc aactgctcta ccaactgagc taacgaccca tacgggtgtt acctgaaaga
      481 tttctactcg gggccaatct ttaagattgg tgtgtcgtgc aggatgactc gcttcgcttg
      541 ccctacgggc cgttgctggc gcaacgttat ccttcacgct taacatctga gtttgatgtt
      601 aaaattggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag tcaactgctc
      661 taccaactga gctaacgacc cgagtggtgg gtgatgacgg gatcgaaccg ccgaccccct
      721 ccttgtaagg gaggtgctct cccagctgag ctaatcaccc gatactgcgc tggatactac
      781 aaactacttc caccgaaccc accgtaatta gtggtgggtc gtgcaggatg actcgcttcg
      841 ctcgccctac gggccgttgc tgacgcaacg ttatcctaca cgcttaacat cggtcttcga
      901 tgctaaaact ggtgggtcgt gcaggattcg aacctgcgac caattgatta aaagtcaact
      961 gctctaccaa ctgagctaac gacccacttt ttcgttgctt tcggtttgtt tgatatcccg
     1021 tggcaacggc ggcatatatt actgatttca gacttgagcg caacaaaaat ttcgatgcag
     1081 atcactcaac tgcttatgat tcgcacgaca cgaccagaaa taatgcgact tctggtcgtg
     1141 tgttatgcat tacatcgcgt tcagacgttt ttgtgcctgt ttagcgccat cagtaccagg
     1201 gtatttactg ataacctgct ggtatacggc tttcgctttt gcggtgtcac ctttgtcctg
     1261 catgatgacg ccgactttaa acatcgcatc agcagccttt ggtgacttcg gatagttttt
     1321 cactaccgaa gcaaaatagt acgccgcatc atctttttta cccttgttgt agtttaactg
     1381 acccagccaa taattggcgt ttggcaggta agttgaatca gggtaatttt tgatgaaatt
     1441 ctgaaatgcc accattgcgt catcctggcg ggatttatcc tgcaccagcg caatagctgc
     1501 attgtaatcc gtgtttgcat caccgctttt caccggcgcg ccagcattcg cagtaccagc
     1561 atcagctgtc ggcgtcgttg atgccgccgc accgctttga tcgctgctgg ttgattgcgc
     1621 cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg atctgctttt gccgctccac
     1681 gacctgattc agttgatact gattttcctg aatctgacca cgcagggaat caatatcgga
     1741 ttgattatca gaaagttgtt gctggagttg ggttaaaagc tggctgtgag cattagaaat
     1801 acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag ccgacactac tgattggtgc
     1861 ctgagcaaaa gcggcccagg gggccgctat accaaccagt aacgacagac tcaatagttg
     1921 atgtctgaag ttactgctca tgcaattctc ttagtaaacc agtaccgcac gacggttttt
     1981 ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt tctttaccgt aagaaacgat
     2041 ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac atcttaacgg cgttcgcacg
     2101 acgttcaccc agggagatgt tgtattccgg agtaccacgt tcgtccgcgt gaccttctac
     2161 ggtgactttg taagacgggt tgctacgcag gaagtttgca tgtgcatcca gcatttgagc
     2221 gaagtcagaa cggatatcgt acttgtccag atcgaagtaa acgatgttgt tctgctgcag
     2281 ctgttgcatt tgcagacgag cctgctcttc ggaagacatg ttgccgttgc cgccgttcgc
     2341 atccatacca gtgccggcac ccagcatgcc ttcgctgccg tcattgctgg cgttcttgtt
     2401 ggaagaacat gccgcaattg ccataacagg cagagcaatc atcagccctt tcagcacttt
     2461 gttcagttgc atttcaatga ttcctttact attcaattaa ttattatcac agatacggcg
     2521 accaggcagg gaatttgacc tgtccatcag ttgccggaag acgcgctttg aaacgcccat
     2581 ctgtagaaac caaattcagc acggatccca tcccctgaga agagctgtag attaccatag
     2641 tgccgttagg tgccagactt ggcgtttcat ccaggaacgt ggacgacaga acttgtacgc
     2701 ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc accattggag ctgaccatta
     2761 ccataaattt accgtcgctg ctgacatccg catcctggtt ctgcgaacct tcccaggtaa
     2821 tacgttgtgg cgcaccgccg ttgatattca ctttataaac ctgcggacga ccggcctggt
     2881 cagaagtaaa tgccaggttc tggctatccg ggaaccaggt cggttcggta ttgttactgc
     2941 gaccatcagt cacctggcgg atctgaccag aagccaaatc cattacgtac aggttcagac
     3001 taccggtttt cgacaaggcg aatgccagtt tgctgccgtc tggcgagaat gcaggtgcac
     3061 cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc atttgccagc gtctgaataa
     3121 ccagcgcgga acgaccgctt tcgaaggtca cataagccag tttagaaccg tctggtgacc
     3181 acgccggaga catcagcggc tgcggtgaac ggtgaacgac aaactggttg taaccgtcat
     3241 agtcagatac gcgcagttca tacgggaact gaccgccgtt ggtctgaaca acgtaggcaa
     3301 tacgggtacg gaacgcacct ttaatgccgg tcagtttttc aaacacttca tcactggcgg
     3361 tatgaccagc ataacgcagc cactgcttgt tcactttgta cgagttctga gcaagtacag
     3421 tacccggtgc gccgccagtg tcaacaagtt gataagcaac attgtaagaa ccatccggat
     3481 tcggagtgac ctgaccgaca actacagcgt caatgcccag tgcggaccat gcagctggtt
     3541 gtacttcctg cgcactaccc ggctgctgtg gcagacgagc gcgatctaac ggattaaatt
     3601 taccgctgtt acgcaagtca gcagcaacga tgccgccaat atcttcaggt gccgcaccag
     3661 gccccgccca ctggaaagga acaacaccaa taggacgacc ggaatctaca ccgctgtcga
     3721 tcacaatgcg gacttcagca tgcagaactg atgcccacag tatgagaaaa ccaaatgcta
     3781 ctcgtaatgc ctgcttcatc atatctccct tatctggacc gatggcccac gataatttag
     3841 cagaatgtta acaaactaaa atacacaaaa ctaccagaac cccgtggcaa ccggtgcctg
     3901 atgttgaccg tccgaacagt caacatcgcg attacggttt gaagtccaat ggcgcgtttt
     3961 tgaacacttc atatactgcc tggcttggtg gtttcgggat cttcgcaagt ttagctgctg
     4021 ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg tttgatatcc agtaacatac
     4081 catcgggtgc cagttttatg cgcagcgtac aggttttgcc tgcataggac gatgcgtcat
     4141 agaacttact ttcgatagca gatttaatct gcccggcata gttattgata tcggcccctg
     4201 atgcgccatt gtttttagta ttaccactcc cggcaggcga agcattgttc cctttcgccc
     4261 ctccccccgt tttcggtgca ttcttaccag agcttagctc accgaaaata tcatctgccc
     4321 ctgcggccgc ttttgctgca gcggcttttt cagctgcagc ttttgccgct gccgcttttt
     4381 tgtctgcggc ggctttttct gccgctgctt tcttatcagc tgctgccttt tcagcagccg
     4441 ctttttctgc tgcttttttg tcggctgcag ctttctctgc tgccgctttc ttatcagctg
     4501 cagccttttc agcagccgct ttcttctcag cttctgcttt ggctttttca gcagcttcag
     4561 ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc cgcttccgct ttcttcttca
     4621 gtgccgcagc ggctgcctcg gcttttttct gcgcttcggc tgcggctttg gcggcttctg
     4681 cttctgcttt tttctttgcg tctgcagccg ctttcttcgc tgcttcttcc gcagctttag
     4741 catctgcttc ggccttcgct ttagcatctg ccgccgcttt cgctgccgcc tcttcagctt
     4801 gcttctgctt taactcggcc tgttttgcgg cttcttcagc ctgctttttc tgctcctgag
     4861 ccgctaaccg ctctttctca agttgcttca ggcgttcctg ttcagccgct tgtttctcac
     4921 gcagttcttc agcagcctgc tgttccttta tcttgcgctg ctcatcagaa cgcttcgcgc
     4981 ttgattcctg gctttgcatg cgtttgtact gctcaactac cgcacctgaa tcaaccatga
     5041 cagcgtcgat ggacgaacca ccgccgcctc cggctgaagc ttctatattc tcatcgaacg
     5101 aactccagat cagcgccgca aataagatga catgcagcac tgctgaaata attatcgccc
     5161 gcttgagctt gtcgttttgt tcggttgcct ttgacactct cggtttccaa aaactgttcg
     5221 cctgttaccc gctctctttc aagcaaggga aacgcagatg tttagatagg ctgcgtcatt
     5281 aaaccaaccg atttcacacc cgcactatgt aacaagttca gtgctttaat tatttcatcg
     5341 taaggcacat cttttgcgcc accgatcaga aagaccgttt tcgggttggc cttgaaacgg
     5401 ctggacactt ccgccaccac ctgctctggt ggtaaacgct ccaggcgatc tttctcaacc
     5461 accacggtgt actgaccaat accagacact tcaacaatca ctggcggatt atcgttactg
     5521 ctcaccgcct gtgattcagt agcgtctggc agatcgacct ccacgctctg ggtgatgatg
     5581 ggcgctgtcg ccataaagat cagcaacagc accagcagta cgtccagcaa cggtacaatg
     5641 ttgatttcgg acttgagatc gcgacgacct cgtccacgcg ctctggccat ggcttacccc
     5701 ttgttgctct cgctaacggt aaacgcctgg cggtgcagaa tcgcggtaaa ctcttccata
     5761 aagttgtcgt aattcagttc cagtttgttt acgcgctggt tgaggcggtt ataagccatt
     5821 accgccggaa ttgcggcgaa cagaccaatt gcagtcgcaa tcaacgcttc tgcgataccg
     5881 ggcgcaacca tttgcagtgt tgcttgtttt accgccccga gggcgataaa ggcgtgcatg
     5941 atcccccaga ccgtaccaaa cagaccaata tacgggctga tggagccaac cgtaccgagg
     6001 aacggaatgt gcgtttccag attttcaagt tcacggttca tggagatacg catagcacgc
     6061 gacgcccctt ccactacggc ttccggcgca tggctattgg cacgatgcag gcgcacaaac
     6121 tctttgaacc cgctgtaaaa gatttgttcc gaaccagtca gattatcccg tttcccctgg
     6181 ctctcttgat agaggcgaga gagttcgatt ccagaccaga atttatcttc aaacgcttcg
     6241 gcttcgcgcg ccgctgcgtt aagaatacgg gtccgctgga taataatggc ccaagatgcg
     6301 attgaaaaac caatcaaaat caacatgata agtttaacca gaaggctagc cttcaggaac
     6361 aaatcaagga tattcatgtc agtcactgct taaactccgc gacaatagac ttgggaagcg
     6421 cacgaggctt cattttgagt gggtcaacgc aaacaaccag aacctctgct tcattcagca
     6481 aagtattctc ggcgttgaca atacgttgcg tgaaaaccaa agaggtgcca cgcattgatg
     6541 ttatttcagt ctgtatttcg agcatatcgt cgagccgcgc aggtgcgtaa tattccaccg
     6601 tcattttacg taccacaaag gcaacgcgtt cagccatcag cgcctgctga ctgaagtgat
     6661 gatgacgcag catctctgtg cgtgctcttt cataaaaagc gacgtaactg gcgtggtaca
     6721 ccacaccacc ggcatcggta tcttcatagt agacgcgaac cggccatcga aacagcgttg
     6781 tattcacttt acatcccggt aatgcaacaa aagttagagc ttttaaacgc tgctactata
     6841 cgcgcgcgat tgaggtttgg gaatgagggc aagttaaggg agcgtaaaaa tttatgggcc
     6901 tggagcggcc cataattttt taaagagaat tctgatttaa aagaagaaga aaatcagccc
     6961 gacaatgaga acaatatcgg caagcaatgg gcaaaaaatc ccttgccaaa gaaccttctg
     7021 cggacgaaag cccacgccgt gaatcacacc agcacagacg gcccacatca gcaataaacc
     7081 atgccagatt tccagttcac tggtcttcgc ggcaaaacga gatgggtccc aaaacataca
     7141 tcctgctaac agaagcgcca tcacgaagga aagcgcccgc agggggcgct tgtccattac
     7201 cgcatacaag gtcgcgataa tcttactcat cagatgtctt cttgaccggc tttgcctgat
     7261 tcgacgtgtt caagcgccag cgcggtgatt accccaaacg aacaggcaag aagcgttccc
     7321 agaatccatg cgaaatacca catttttagc tccttactta gtacagagag tgggtgttac
     7381 gttcaatatc ttctttggtg atacgaccga acattttcca gtaacaccag gcggtgtaga
     7441 gcagaatgat cggtaccaga accaccgcaa cccaggtcat gacgttaagc gtcagctggc
     7501 tggaagttgc atcccacatt gtcagacttg cgttcatcat ggtgctggac ggcatcacaa
     7561 acgggaacat tgcgataccg gctgtcagga tgatgcaggc cagcgtcagg gaggagaaca
     7621 caaacgccca cgcggcttta tccatacgtg cagtcaggat ggtcagcagc ggcagaacca
     7681 cacccagtgc cggaatagcc cacagaattg gcgtgttgtt gaagttaacc agccatgcgc
     7741 cagcttcacg aaccacttct ttattcagtg ggttagaggc tgcgtaatgg tccattgtcg
     7801 atttcacgac ataaccatcg ataccgtaca tcacccatac gccagccagt gcgaaacaga
     7861 ccagtgtcac cagcgcagcc acctgagccg ttgcacgggt acgcaggtgc agttcgccca
     7921 cggtacgcat ttgcagatag gttgcgccct gagtaatgat catccctacg ctcaccacgc
     7981 ctgccagcag gccgaacggg ttaagcaact ggaagaagtt accggtgtag tacagacgca
     8041 gatattcatc aacgttgaac ggtacgccct gcaacaggtt accgaacgct acaccaatta
     8101 ccagcggcgg aacgaagcta ccaatgaaga tgccccagtc ccacatgtta cgccagcggg
     8161 tttcttcaat cttggagcgg tagtcaaaac cgaccggacg gaagaacaaa gacgccagca
     8221 cgaggatcat cgccacatag aagccggaga acgcagcggc atagaccatc ggccaggcag
     8281 caaagagtgc gccgcccgcg gtgatcagcc aaacctggtt accgtcccag tgtggtgcaa
     8341 tggagttaat cataattcga cgctcggtgt cgttacgacc gaggaaacgg gtgagcatgc
     8401 ccacccccat gtcgaaaccg tcagtgactg caaaaccaat cagcagaacg ccaaccagca
     8461 gccaccagat aaaacgcaat acttcataat cgatcatttg acgactcctg tcttagcgtg
     8521 ccggctgagt agtcgtggaa gactgctcaa agtgatagcg accggttttc aggctgcttg
     8581 ggccgaggcg tgcaaatttg aacattaaga acaattctgc caccaggaac agggtataca
     8641 ggccgcaaat cagcaccatt gagaagatga gatcgcctgc ggtcagtgac gaattcgcca
     8701 cagctgtcgg cagcacttca ccgatagccc acggttggcg gccatattca gccacgaacc
     8761 agcccgcttc tacagcaatc cacggcagcg gaataccgta cagcgcggcg cgcagcagcc
     8821 attttttctc gccaatgcgg ttgcggatga cactccagaa agagagcgcg atgattgcca
     8881 gcagcaggaa gccacacgcc accataatac ggaacgcgaa gtacagcggc gctacacgcg
     8941 gaatggagtc tttggttgcc tgttgaatct gcgcttcagt cgcatcagcc acgtttggcg
     9001 tatagcgttt cagcagcaga ccgtaaccga ggtctttctt catgctattg aactggtcac
     9061 gaaccgcctg gtcggtagaa ccagaacgca gttgttcgag cagagagtac gccttcatcc
     9121 cgttacgaat gcgttcttca tgctgcacca tcagctcttt caggccgata accggggtat
     9181 ccacggaacg cgttgcaatg atgcccagtg cgtaagggat ctgaatcgca aatttgttcg
     9241 tctcctcttc ctgatcagga atgccgaaca gagtaaaggc agcaggcgca ggttgcgttt
     9301 cccactcggc ttcaatagca gccagtttgg ttttctgcac gtcgcccatt tcgtagccgg
     9361 attcatcacc cagaacaata acagacagaa cagcagccat accgaagctg gcagcgatag
     9421 caaaggagcg tttagcgaag gcgaaatcac gacctttcag catataccat gcgctgatac
     9481 cgaggatgaa catcgcgcca gtcacataac cagacgctac agtgtgaacg aatttcacct
     9541 gagcaaccgg gttaagcacc agctcggaga agctcaccat ctccatacgc atagtttcaa
     9601 agttgaaatc ggacgcgatt gggttttgca tccagccgtt cgcaaccaga atccacagtg
     9661 cggacaggtt tgaaccgagc gccaccagcc aggtgacaca catatgctga actttaccca
     9721 gacgatccca accgaagaag aacagaccta caaaggtgga ttcgaggaag aaggccatca
     9781 gaccttcgat tgccagcggc gcaccgaaga tatcccctac atagtgggaa tagtaagacc
     9841 agttagtccc gaactggaac tccatggtca gaccggtagc cacacccaga gcgaagttga
     9901 taccaaacaa cttgccccag aacttggtca tatctttata aatctgtttg ccggagagga
     9961 cgtagaccgt ttccataatg gccagcagga acgccatacc gagcgtcagt ggcacaaaaa
    10021 ggaagtggta catcgcggtc aaggcaaact gtaagcgcga cagttcgact atatctaaca
    10081 tcatgactcc ttgctcatcg catgaagact ccgagagtga accccgttag aaagggtcac
    10141 acgcatgccc caatagaaat ttgtttgaac actttcatca taccatgtga atgcacacaa
    10201 aaacggcgta aagaaaggtt gcaaacatgt taataaaaac tcaaattgat cccacgtata
    10261 tattacgccg caaaatcctt acaataaaca ggtttttgtt gaagcaaatt tgcatttttc
    10321 gacggtgatc aat
//
LOCUS       DAZKHC020000129        10221 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZKHC020000129 DAZKHC020000000
VERSION     DAZKHC020000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.129"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(100..345)
                     /locus_tag="ACHOSM_RS20465"
                     /old_locus_tag="pgaptmp_004093"
                     /pseudo
     CDS             complement(100..345)
                     /locus_tag="ACHOSM_RS20465"
                     /old_locus_tag="pgaptmp_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141912.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(355..615)
                     /locus_tag="ACHOSM_RS20470"
                     /old_locus_tag="pgaptmp_004094"
     CDS             complement(355..615)
                     /locus_tag="ACHOSM_RS20470"
                     /old_locus_tag="pgaptmp_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560476.1"
                     /translation="MCESNDKNLTIYYVLPDEQYRPDLLAYRMWGIAELRWVVTLAAG
                     LEDESQGMTVGKKLKLPPATWIREMIRHFQYDGQVIGTLSIA"
     gene            879..1124
                     /locus_tag="ACHOSM_RS20475"
                     /old_locus_tag="pgaptmp_004095"
     CDS             879..1124
                     /locus_tag="ACHOSM_RS20475"
                     /old_locus_tag="pgaptmp_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560477.1"
                     /translation="MAERVDDAELSMNQLEALKDMAIDNIRKQAQVVSQVFTGKCRYC
                     NESIESGINCDAECAQWHREEQAAKQRKYGMRPAGFD"
     gene            1127..1705
                     /locus_tag="ACHOSM_RS20480"
                     /old_locus_tag="pgaptmp_004096"
     CDS             1127..1705
                     /locus_tag="ACHOSM_RS20480"
                     /old_locus_tag="pgaptmp_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HGU4560478.1"
                     /translation="MLRFTEEEFQAFSERRNKGRSRPKTKKDPFLSLAPVKEVSPHAK
                     ALAALAKNPDLRDGNCEHFEQVFIFDYFERKHPDIYELLHATPNGGKRSKATAGKMKA
                     EGQKKGYPDMSLDKACGIYHGMRIELKEPNGKAPTKEQIAWMRRLREEGYYVVLAYGA
                     EQAITAILEYISLKKGEAIEHVLNGDKWLYVA"
     gene            1772..1927
                     /locus_tag="ACHOSM_RS20485"
                     /old_locus_tag="pgaptmp_004097"
     CDS             1772..1927
                     /locus_tag="ACHOSM_RS20485"
                     /old_locus_tag="pgaptmp_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560479.1"
                     /translation="MSSKANYESLASIMPRNEQETDAVVDPVIAEMNARLEAEFAAEN
                     EHTTQGD"
     gene            2031..2531
                     /locus_tag="ACHOSM_RS20490"
                     /old_locus_tag="pgaptmp_004098"
     CDS             2031..2531
                     /locus_tag="ACHOSM_RS20490"
                     /old_locus_tag="pgaptmp_004098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560480.1"
                     /translation="MFDDIYALYAGDLSWWKQYGSTIPGGRFRKVTANLAAAKSFSLE
                     YRRYCGPAEGVNSGAQAISLAAESGAEVVVLVGYDCSLQNGLHWHGAHPQALRNPTQV
                     SISKWQQQFLDTRKKHADLHILNASRSSAIQCFPRINLEAVIALLSSAVAQAPQTLLR
                     RAECRL"
     gene            2429..3043
                     /locus_tag="ACHOSM_RS20495"
                     /old_locus_tag="pgaptmp_004099"
     CDS             2429..3043
                     /locus_tag="ACHOSM_RS20495"
                     /old_locus_tag="pgaptmp_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560481.1"
                     /translation="MFPKNKFRGSDRVIIVGSGPSAANFVAPRGVPIIAVNGAIDWLN
                     RASYFFTLDPSPDNMRRVGRGRRRRGVCYCMALPDVKEREVRDGVLCFRRVAERGMEH
                     SPEWWAWRWSAHFGLCEDENEIASGNSAYGALNLAFHIGFKHVALVGVDATQEPRVHS
                     GGTPKNLSHLPLLFQSARERIDVVSCGKMGGIPQMTLKEWLKNT"
     gene            3040..3717
                     /locus_tag="ACHOSM_RS20500"
                     /old_locus_tag="pgaptmp_004100"
     CDS             3040..3717
                     /locus_tag="ACHOSM_RS20500"
                     /old_locus_tag="pgaptmp_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGU4560482.1"
                     /translation="MMAPTIYHRIDGTKYRNVWVVGDLHGCYTRLMSELHRVDFDPAQ
                     DLLISVGDLIDRGTENVECLELLQMPWFRAVMGNHERLMIDALSPDGNVNNWLMNGGQ
                     WFFMLDTDQEILAWALVELVKRLPYIIELNTGQETIVIAHADYPDNEYQFGKEVPLFN
                     VVWARERISDSMDDIGGEISGADRFIFGHTPVKSPKTFWNQQYIDTGAVFCGNLTLMK
                     VKGDGAA"
     gene            3714..4415
                     /locus_tag="ACHOSM_RS20505"
                     /old_locus_tag="pgaptmp_004101"
     CDS             3714..4415
                     /locus_tag="ACHOSM_RS20505"
                     /old_locus_tag="pgaptmp_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560483.1"
                     /translation="MKIALVFRSGGDYNASDVQWLVNQLPKGYEIICLTDLKRLHVPG
                     VKVVPLINQWQKCRGWWAKIELFRPDITDDLFYLDLDTVIAGDIRPILEHPPTSFTML
                     RDFYHPQYRGSGALWIPNSVKAHIWSSFWQDPEGWISRCVTTECWGDQGFLRKVMGDD
                     TPAFQDLYPGWFVSYKADVVEPGSKYASARYSRGNGALPKDCRIIFFHGKPRPREVSE
                     DWLPLVSSFFERESE"
     gene            4717..5979
                     /locus_tag="ACHOSM_RS20510"
                     /old_locus_tag="pgaptmp_004102"
     CDS             4717..5979
                     /locus_tag="ACHOSM_RS20510"
                     /old_locus_tag="pgaptmp_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560484.1"
                     /translation="MSTSAQNQSIENVSIPDVLNAGIPAIIQNIRAAQRRVSCDDLTA
                     RFFDNAVQSAEMLHAQLIDVYNAEADSHNSLVDAAENMQLDLGLKGKEIEELQLQIEH
                     LKRQQQDAIDDATHDANQRADNAERISIELETKLNEMTAMVELRNSQISTLKSQYKEI
                     MKLDPFNLEKRYNKAKSERQELRKQVADLNQQLKKTIKDASEARVAFANKKAEVTALV
                     NENAKFATLKKEMYGITERRFPARKLHPTLGQISFFPRLLAYGISSPKEFNNERPYIV
                     SKLDFAYQFCCDMGYAIDIRINEWLMPNFQPLAIFREFQPEGWVEFFHELICKEMESR
                     RPELVRRVEWAQEVMLAEAELPFEPEFIDDLATKGLHTLFDVVTRRHEQLVVELGLEE
                     TAARRLLDVCYARSDAWEKENGGTIYVR"
     gene            6052..6567
                     /locus_tag="ACHOSM_RS20515"
                     /old_locus_tag="pgaptmp_004103"
     CDS             6052..6567
                     /locus_tag="ACHOSM_RS20515"
                     /old_locus_tag="pgaptmp_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-phosphatase"
                     /protein_id="HGU4560485.1"
                     /translation="MSIFNKHAHQERPYIVIVDIDGTISEATEDRLHLLPPPGKGALT
                     EHWNEFNLACDTDAPITPVIDMVRQLFNVYTVWFVTGRCEIARDKTRAWLRKYVTNGA
                     EPLLSMRPATDDRNDGPAKIDLLKKIGLSKIAFALEDKIEVARVFRSHGVLTLMVREY
                     ENALLYQQQLL"
     gene            6754..7479
                     /locus_tag="ACHOSM_RS20520"
                     /old_locus_tag="pgaptmp_004104"
     CDS             6754..7479
                     /locus_tag="ACHOSM_RS20520"
                     /old_locus_tag="pgaptmp_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560486.1"
                     /translation="MKGEVKERGMIFNDEMVRAILEGRKTQTRRIMKNQPAEVGPEAP
                     VMVRKIGAGFQWYGADGVSSVFNCPFGIVGDRIWVRETWAILGNEDGCSVDWNDNLCR
                     GDEKNAARIYRASCEQKPGDYGLWSIPDDADWKPHTVNEKFDGGWRPSIHMPRWASRI
                     LVEITNVRVERLNDISECDARDEGVPPAGSLLPDHPGTFLTPKGDFAMAKVAFQRLWE
                     SIYGEESWSANPWVWVIEFERIQ"
     gene            7519..7710
                     /locus_tag="ACHOSM_RS20525"
                     /old_locus_tag="pgaptmp_004105"
     CDS             7519..7710
                     /locus_tag="ACHOSM_RS20525"
                     /old_locus_tag="pgaptmp_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HGU4560487.1"
                     /translation="MNKASPAELRASLEMAHSLAQIGVRFVPIPVETDEEFHRLAASA
                     AQKLEIMAAKVEKAEGATK"
     gene            7707..7922
                     /locus_tag="ACHOSM_RS20530"
                     /old_locus_tag="pgaptmp_004106"
     CDS             7707..7922
                     /locus_tag="ACHOSM_RS20530"
                     /old_locus_tag="pgaptmp_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560488.1"
                     /translation="MSKPTDEEIVRVLEEHGRCMTYVVTNWLRDKYRTLKTAYVLRRL
                     KKLEFDGKVKRVNSSYIRQICWEASSE"
     gene            7915..8559
                     /locus_tag="ACHOSM_RS20535"
                     /old_locus_tag="pgaptmp_004107"
     CDS             7915..8559
                     /locus_tag="ACHOSM_RS20535"
                     /old_locus_tag="pgaptmp_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560489.1"
                     /translation="MNNRFYMMCLRETVGNNASFHCHNGNGYSSDIDRAHVYTLEAAQ
                     KAWNCGRDIDQPVCADSVDAMAVWHVDCQYIPTESLIESDCTAYVAYKKGSWNGNDVY
                     WLQHGGLPTDDFSKATIFSVANKNEPGIVWLPFSIADAAKRRTFNINNFNRRTMVQGA
                     GLVMPDWLKKQNRRKKSRSGKVRWNCPHCGKITWQYSPYDFEGCSDYNCEGWRE"
     gene            8556..9422
                     /locus_tag="ACHOSM_RS20540"
                     /old_locus_tag="pgaptmp_004108"
     CDS             8556..9422
                     /locus_tag="ACHOSM_RS20540"
                     /old_locus_tag="pgaptmp_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4560490.1"
                     /translation="MTIDYQVLREAAEKATPDEWVAFISTDTGTYAVHTPGDERCEDV
                     IKWTGFDGQKNAENNARYVAAFNPKVALELLGEIKRLEDTNIDAMCRIAELETNLAAL
                     VAENAGLKAGAMYFSYGSEFSFECHKTAEEAIAAAEAAIDDYRGDACDGWSEEVESIC
                     WGVIIQQATKVGERKKRKCDRVSPWIERVCDYELRPNVETPATDAFLAEVRAEARNEG
                     INYTASRLAAAFNHGFINKSLREVFDVTRMILSAKEELANEAHPIDGLSGEYAEKSLE
                     EWAEQIRKGSSQ"
     gene            9419..9709
                     /locus_tag="ACHOSM_RS20545"
                     /old_locus_tag="pgaptmp_004109"
     CDS             9419..9709
                     /locus_tag="ACHOSM_RS20545"
                     /old_locus_tag="pgaptmp_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HGU4560491.1"
                     /translation="MNIDTTITIDTLLNTGLALLGWLYIMSRTWRWLGSIFLKQWKKR
                     RKQELRQKALEAFYDAFELSRIEPGTTARIATKGDLMIVMFRQERAEKGESA"
     gene            9706..>10221
                     /locus_tag="ACHOSM_RS20550"
                     /old_locus_tag="pgaptmp_004110"
     CDS             9706..>10221
                     /locus_tag="ACHOSM_RS20550"
                     /old_locus_tag="pgaptmp_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560492.1"
                     /translation="MKFSKFSELVNRILSNNHSHRRDMNVTIVVHSPGSIGSTPSVEV
                     QSIHAGFDWDSGKVLIFPSQPLTTLTPEQITDITDSVRKGQSWHAYQEYKKHQEQLEK
                     LSIELDAAKQRIAELEGNRTALAVENELARKAVQAFCDVVGDNTEVIAEVVGRDGVLV
                     ILEAMKATGNMP"
BASE COUNT         2784 a         2185 c         2729 g         2523 t
ORIGIN      
        1 ggcagaagca gaaaaagctt attatgcttc catcagaaac gatgatctgg cagcctgagt
       61 tcacagataa aatactctcc aggaaacccg gggcggttca gtgttgaaac tgcgtatatc
      121 ttcgggcgta aagtaggtgc catctttctt tttcccaagg ctgaggaatg cctcaagttc
      181 gcggttacgc atccccataa tccttggggt gagtgtacgt cgcgcgtttc gccgattctg
      241 acgctgctgt ttacggataa gatcgaagac cttgttaaag tcttttgcac ttaatccatc
      301 agtctgatag cgaccaaggt tgtcgcgagc atattcagtt ggcattcatt tcccttacgc
      361 aatggataat gtccctatta cctggccgtc gtattggaaa tggcgaatca tttcgcggat
      421 ccaggtggca ggtgggagtt ttaatttttt gccaacagtc ataccctgag actcatcctc
      481 aagcccggcg gcgagcgtca caacccagcg tagctctgct atgccccaca tacggtaagc
      541 cagcaaatcc gggcgatatt gctcatcggg aagaacgtaa taaatcgtca gattcttgtc
      601 gttcgattca cacataagca tcacctcttt gcgtagctct gccctgagta ttggatcggc
      661 tatgttgcgg tcgtcatacc gcgacagagg atattgccgg gtgctttggg ttgtagtgat
      721 tgatgtagcc atagtcagcc tgccagaaat agatgatggt gattctaccg ctagtcattt
      781 gttgaatatt taactcaata aaagaaaatt attagtgcaa ttttgaatgt gaaatgtatc
      841 attctgccct taagtaggtt cttcacgagg aaacaaaatt ggcagaacgt gttgatgatg
      901 cagagctgag catgaatcag ttagaagctc tcaaagacat ggccatcgat aacatcagaa
      961 agcaggcaca ggtcgtgagc caggtattta cagggaagtg tcgttactgc aatgaatcga
     1021 ttgaatcagg cattaattgt gacgctgaat gtgcgcaatg gcacagggaa gagcaggccg
     1081 caaaacagcg taaatatggc atgcgaccgg caggatttga ctgattatgt tgcgctttac
     1141 tgaggaagag tttcaggctt ttagtgagcg tcgaaataag gggcggtcca ggccaaaaac
     1201 caaaaaggat ccattcttat cgcttgcgcc ggtaaaagaa gtttctccac atgcgaaggc
     1261 acttgcagca ctggcaaaga acccagacct gcgcgacgga aattgcgagc acttcgagca
     1321 ggttttcatt tttgattact tcgaacgcaa gcaccctgac atctatgagc tgttgcatgc
     1381 aacgcctaac ggagggaaac gttcaaaagc aaccgccggg aaaatgaagg ctgaagggca
     1441 gaaaaaaggt tatccggaca tgagtctcga taaagcatgc ggtatttatc acggcatgcg
     1501 aattgagctt aaagaaccaa atggtaaagc cccgacgaaa gagcagatcg cctggatgcg
     1561 caggcttaga gaggaaggtt actacgtcgt tcttgcgtat ggtgcagaac aagcgataac
     1621 cgccatcctg gaatacataa gccttaaaaa gggtgaggct attgagcatg tattgaacgg
     1681 cgacaagtgg ttgtatgttg cttaaaataa taaattaatt agtacatatg agccatttga
     1741 tatagcgcac attaacatcg ggagaataat cgtgtcatcc aaggctaatt atgaatcgct
     1801 ggcatcgatc atgccgcgta atgaacagga aacagatgct gtagtggacc ctgtaatcgc
     1861 tgaaatgaat gctcgcctgg aggctgaatt tgcagctgag aatgaacata ccacgcaggg
     1921 cgactaggac tgttttttgt gtcggtagcg gtccgtcact cactcgtgag gactgtgctg
     1981 ctatagaaaa aactggctgt tcaatcatcg cggttaacaa ttcctggcag atgttcgatg
     2041 acatttatgc cttatacgcc ggtgatttgt catggtggaa gcaatacgga tccaccatac
     2101 cgggagggag attccgcaaa gtgacagcca acctggcggc ggcgaaatca ttttcgttgg
     2161 agtacaggcg atattgtgga ccggcggaag gggtaaatag cggcgcgcag gctatcagtc
     2221 tggctgctga atcaggggct gaagtagtgg tattagtcgg ctatgactgt tctctgcaaa
     2281 acggccttca ttggcatggc gcgcaccctc aagccctacg gaatccaacg caggtttcta
     2341 tttcaaaatg gcaacagcag ttcctggata cccgcaaaaa acacgcagat ttacatattt
     2401 tgaatgcaag taggagcagt gcaattcaat gtttcccaag aataaattta gaggcagtga
     2461 tcgcgttatt atcgtcggca gtggcccaag cgccgcaaac tttgttgcgc cgcgcggagt
     2521 gccgattata gcggtcaatg gggccatcga ctggctgaac cgcgcttctt attttttcac
     2581 acttgatcca tcgccagaca atatgcggcg cgttggtcgt ggccgccgtc gccgtggtgt
     2641 ttgttattgc atggcactac ccgatgttaa agaacgtgaa gtcagagacg gcgttctgtg
     2701 cttccgtcgt gtggctgaac gtggcatgga gcattctccc gagtggtggg cgtggcgctg
     2761 gtccgcacat ttcggacttt gcgaagatga gaatgaaatt gccagcggca atagtgcata
     2821 tggcgctctg aacctggctt tccatatcgg attcaaacat gtcgccctgg tgggcgttga
     2881 cgctacacaa gaaccacgcg ttcactccgg cggcacgcca aaaaatctaa gtcacctgcc
     2941 tttgttattc cagtctgcgc gtgaacggat tgacgttgtt tcatgcggga aaatgggagg
     3001 tattccgcag atgactctta aagaatggct gaagaataca tgatggcacc cacaatttat
     3061 caccgtatcg acggtaccaa atacaggaat gtctgggttg ttggtgatct gcatggttgc
     3121 tacaccagac tgatgtccga actccatcgt gtggattttg acccggcgca ggatttactg
     3181 atatcggtcg gcgaccttat cgatcgcggt actgaaaatg tcgaatgtct ggaactattg
     3241 cagatgccct ggttcagggc agtgatgggg aaccatgagc ggctgatgat tgatgcgtta
     3301 agtccagatg gcaacgtgaa taactggcta atgaatggcg gacaatggtt cttcatgctg
     3361 gacactgatc aggaaatatt agcctgggcg ctggtggagc tggtaaagcg tctgccctat
     3421 atcattgagt tgaacaccgg gcaagaaact atcgttatag cccatgccga ctatccggat
     3481 aatgaatacc aattcggtaa ggaggtgccg cttttcaacg ttgtctgggc gcgcgagcgt
     3541 atcagtgatt cgatggatga tattggtggc gaaatttcgg gcgcagatcg ttttatcttt
     3601 ggtcacactc cggtgaaaag cccgaagaca ttctggaatc agcagtatat cgacactggt
     3661 gccgtatttt gcggaaacct gacattgatg aaagtgaaag gtgatggtgc agcatgaaga
     3721 ttgctttagt ttttcgctct ggtggtgact ataacgcttc cgatgtgcag tggctggtta
     3781 atcaactgcc aaaaggctat gaaattattt gcctgacaga cctgaagcgt ttacatgtac
     3841 ctggcgtcaa agttgtccca ttgatcaacc agtggcaaaa gtgccgtggc tggtgggcga
     3901 aaatcgagtt gttccgaccg gatataaccg atgatctgtt ctatctggat ttggacacgg
     3961 ttattgccgg tgatatacgc ccaatcctag agcatccacc aaccagcttc accatgctta
     4021 gggattttta ccatccacaa tatcgtggca gcggtgccct gtggatacca aatagtgtta
     4081 aagcgcatat ctggagttca ttctggcaag atccggaagg ttggatttct cgttgtgtca
     4141 ctactgagtg ctggggtgat caggggttct tacggaaggt tatgggcgat gatacaccag
     4201 catttcagga tctgtatcca ggatggtttg taagttacaa ggccgatgtt gtggaacctg
     4261 gttcaaaata tgcgagcgcg cgttactcca gggggaatgg ggcattacca aaagactgcc
     4321 gaataatctt tttccacggc aaaccgcgac ctcgcgaagt gtcagaggat tggcttcccc
     4381 ttgtcagctc attttttgag cgagaatcag aataatattg ctctaataat tccatatttt
     4441 taaagcgtga tgtacactca tcacgttttt tattagagca atctacaagg tgcactatgt
     4501 ggccattccg acggaaatat cactactggc tgatcgcctt tgttacgccg accggcggta
     4561 tcaggcatgt catcaccagg tatcgcaaca agagactcac cttagccaga attttacagg
     4621 ctgccatagg tgagggactg gatacaaatt gcgtagtcct tcctccttca tacttaggaa
     4681 aaatgaccga agcacaagct aatacggaac tttgaaatga gcacttcagc acaaaaccaa
     4741 tcaatcgaaa atgtatctat ccctgacgtc ctgaatgccg gtatcccggc cattatccag
     4801 aacatccggg ccgcgcaacg ccgcgttagt tgtgatgacc tcacagcgcg tttttttgat
     4861 aatgcggttc agtcagcgga gatgcttcac gcacagctta ttgatgttta taacgcagaa
     4921 gctgatagcc ataactccct ggtagatgca gctgaaaata tgcagttgga tctcggtctg
     4981 aagggtaaag aaattgaaga gcttcagctg caaattgaac atttgaaacg ccagcaacag
     5041 gacgcgatcg acgatgcgac gcatgacgcc aaccagcgtg ctgataatgc cgaacgtata
     5101 agcattgagc tggaaacaaa actcaatgaa atgaccgcga tggttgaact gcggaactca
     5161 cagatttcaa cgctaaaatc tcaatataaa gagatcatga aacttgatcc ttttaacctt
     5221 gagaaacgct ataacaaagc taaaagcgag cgacaggaac tgcgtaagca ggtcgccgac
     5281 cttaaccaac agctcaaaaa aactattaaa gatgcaagcg aagcgcgcgt ggcatttgct
     5341 aataaaaaag cagaggttac cgcgctggtt aatgagaatg ccaaatttgc gacgctcaag
     5401 aaggaaatgt atggcattac tgagcgccgt ttccctgcaa gaaaacttca tccgacgtta
     5461 gggcaaatct ccttcttccc gcgcctcctg gcttatggga tctcatcgcc taaagagttc
     5521 aataacgagc gtccttatat cgtttctaag ctggactttg cttatcagtt ctgctgcgac
     5581 atgggctatg ccattgatat ccggatcaac gaatggttga tgccaaactt ccagccgttg
     5641 gccattttcc gcgagttcca gccggaaggt tgggtagagt tcttccatga attgatctgt
     5701 aaagagatgg aaagccgccg cccggaactg gtccgtcgag ttgagtgggc gcaagaggtt
     5761 atgttggcag aggcagagct gccgttcgaa ccggaattca ttgatgatct ggcaactaaa
     5821 gggctgcata ccctgtttga tgtggttacc cgccgtcatg agcagttggt tgtcgaattg
     5881 ggtttagagg aaactgcggc aagaagactt ctcgatgttt gctatgcacg tagcgatgca
     5941 tgggaaaaag agaacggcgg cactatttac gttcgctgat agttacagtg tcacttttaa
     6001 tgctggtgga gtgcgcccac cagcattttt ttcgtccaat gaggagggca tttgagtatt
     6061 ttcaataaac acgcacacca ggaacgtccg tacatcgtca tagtagatat tgatgggacg
     6121 atatcagagg caacggaaga caggctgcat ttacttccac cacctggcaa aggtgcatta
     6181 acagagcact ggaacgagtt taaccttgcc tgtgacactg atgctcccat cactccagtt
     6241 attgatatgg tgcgccagtt atttaacgtt tacacggtct ggtttgtaac cgggcgctgt
     6301 gagatcgcaa gggataaaac acgagcctgg ctgcggaagt acgtaacaaa cggggctgag
     6361 cctttgctat ctatgcgtcc tgccaccgat gacagaaatg acggtccagc aaagattgat
     6421 ctcctgaaga aaattggtct aagtaaaatt gcgttcgcgc tggaagataa gattgaagtg
     6481 gcgcgtgtgt tcaggagtca tggcgtactt acattaatgg tcagggagta tgaaaatgcg
     6541 cttctttatc aacaacaatt gctctaataa atattgattt ttaagacagg ggaagtgaaa
     6601 ataaaaacat gccgcaaggc gcggcatgta tccaatcaat cacaggagct gaaaatatga
     6661 acacggcatt caaaatcatt atggccgcga tctatttctg gctgttctct atcacttttg
     6721 gcggcatcgt cgcgcatggg taaaggggga tgcatgaaag gcgaagtgaa agagcgcggc
     6781 atgattttca acgatgagat ggtccgggcc atcctcgaag gaaggaaaac gcagacgcgg
     6841 cggataatga aaaatcagcc tgcggaagtt ggtccagaag caccggtgat ggttagaaaa
     6901 attggtgcag gttttcagtg gtacggggct gatggtgtaa gcagtgtttt caattgcccc
     6961 tttggtatcg tcggcgatcg aatttgggtt cgtgaaacat gggcgatatt aggcaatgaa
     7021 gatggttgca gtgtggactg gaacgacaac ctttgtcgtg gcgatgagaa gaacgcagca
     7081 aggatttatc gggccagttg tgagcaaaag cctggtgatt acggtttatg gtcgataccc
     7141 gatgatgccg actggaaacc tcacactgtg aatgaaaagt ttgatggtgg gtggcgtcca
     7201 tcaattcaca tgccgcgatg ggcatcacgc atcctggtgg aaattaccaa cgtgcgcgtt
     7261 gagcggttga acgatatcag cgaatgcgat gcaagggatg agggcgttcc gcctgctgga
     7321 agtttgcttc ctgatcaccc gggaacattc cttactccca agggggattt cgcaatggcc
     7381 aaggttgcgt tccagcgcct gtgggaatcc atctacggcg aagaaagctg gagtgctaat
     7441 ccctgggttt gggtaatcga gtttgagcgt attcagtagg gcgagcgtat gcaatcagtt
     7501 atttgtgagg aagtcggcct gaataaagca tccccagcag agttaagagc aagtctcgaa
     7561 atggcgcata gccttgctca aattggtgtc aggtttgtac caattccagt tgaaacagat
     7621 gaagaatttc acaggttagc ggcatcggca gcacaaaagc tggaaatcat ggcagcgaaa
     7681 gtagagaaag ctgaaggagc gacaaaatga gcaagccaac cgacgaagaa atagttcggg
     7741 tgttggaaga acacgggcgc tgtatgactt acgtcgtgac caactggctt agggataaat
     7801 atcgcacact caaaacggca tacgttttgc gtcgtctaaa gaaactggag ttcgacggga
     7861 aagtaaagcg cgttaacagt tcttatataa gacaaatttg ctgggaggca tccagtgaat
     7921 aaccgctttt acatgatgtg cttgcgtgaa actgtgggta ataacgcctc attccattgc
     7981 cataacggca atggttacag ttctgatatc gatcgcgctc atgtttacac gctggaagca
     8041 gcccaaaaag cctggaattg tggacgagat atcgatcagc ctgtttgcgc tgatagcgtg
     8101 gatgcaatgg ctgtgtggca cgttgattgc cagtacatcc ctacagaaag cctgattgag
     8161 tcagattgca ctgcgtatgt ggcctacaaa aaaggtagct ggaacggcaa cgatgtttac
     8221 tggcttcaac acggtggatt gccaacagat gacttcagta aagcgaccat ctttagcgtc
     8281 gccaacaaaa acgaaccagg aatagtttgg ttgccatttt ccattgctga tgcagcaaag
     8341 cgccggacgt tcaatatcaa taactttaac cgcagaacaa tggttcaggg cgcaggtttg
     8401 gtcatgcctg actggttgaa aaagcaaaac agaagaaaga agtcgcgaag cgggaaggtg
     8461 cgttggaatt gtccgcattg cggaaaaata acctggcagt acagcccata tgattttgaa
     8521 ggctgtagtg attacaactg tgaaggatgg cgagaatgac aattgactat caggtactgc
     8581 gtgaggcggc agaaaaggca acaccagacg aatgggtcgc atttatttcg acagatactg
     8641 gtacttatgc ggtgcacacg cccggtgatg aacgatgtga agacgttatc aaatggaccg
     8701 gctttgatgg acagaaaaat gcagagaaca acgctcgtta tgttgccgcg ttcaacccaa
     8761 aggttgcact ggagctgctt ggtgaaatta agcgcctgga ggacacaaat attgatgcta
     8821 tgtgtcgaat tgcagagctt gagactaatc tcgcggcgct ggtggcggag aatgcggggc
     8881 ttaaggccgg ggctatgtat ttctcatatg gctctgaatt tagtttcgag tgtcacaaaa
     8941 ctgctgagga ggctatcgct gctgctgagg ctgcaattga cgactataga ggtgatgctt
     9001 gcgatggatg gagcgaagag gtcgaaagca tttgctgggg ggtaattatt cagcaggcaa
     9061 ccaaggtcgg tgaacgcaag aagaggaaat gcgacagagt atcaccatgg attgaaagag
     9121 tttgtgatta tgagcttcga cctaatgtcg aaaccccagc caccgatgct ttccttgctg
     9181 aagtacgtgc ggaagcacgc aacgagggga ttaactatac cgccagccgt cttgctgctg
     9241 cgttcaatca cggatttatc aataagtctt tgcgtgaagt tttcgacgtt acacgcatga
     9301 ttttgtcagc gaaagaagag ttggctaatg aagcgcatcc gattgatggc ctgtctggtg
     9361 aatatgcgga gaaatccctt gaagaatggg cggaacagat tcgcaaagga agcagccagt
     9421 gaatatcgac acgacaataa cgatcgatac gctcctaaat accggtctgg cacttctcgg
     9481 ttggctttac atcatgtccc gtacatggcg atggctgggt tccattttcc taaaacagtg
     9541 gaaaaaacgg cgcaaacagg aactacgcca gaaggcatta gaagcgttct atgacgcatt
     9601 tgagcttagc cgcattgaac caggtacaac agccaggata gcgacaaaag gcgacctgat
     9661 gatagtgatg ttccgacagg agagagcaga gaaaggggaa tcagcatgaa attttccaaa
     9721 ttttctgagt tggtgaatcg tattttgtcc aacaaccaca gccatcgtcg cgatatgaat
     9781 gtaacgatcg ttgttcattc gcctggcagc attggttcaa caccttcagt tgaggttcag
     9841 tcaattcacg ctggttttga ttgggattcc gggaaagtgc ttattttccc atcacagcca
     9901 ctgaccacgc taacaccaga acagattact gatatcactg atagtgtgcg caaaggtcag
     9961 tcttggcacg catatcagga atacaagaag catcaagagc agttggaaaa attgtcgatt
    10021 gaactggatg ccgcaaaaca gcgcattgca gagctggagg gtaatcgcac ggcgctggca
    10081 gtggagaatg agctggctcg taaggcagtt caggcattct gcgatgttgt tggcgacaac
    10141 accgaggtta tcgctgaggt ggttgggcga gatggcgttc tggttatttt ggaggccatg
    10201 aaggcaacag gaaatatgcc a
//
LOCUS       DAZKHC020000130        10167 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZKHC020000130 DAZKHC020000000
VERSION     DAZKHC020000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.130"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..488
                     /locus_tag="ACHOSM_RS20555"
                     /old_locus_tag="pgaptmp_004111"
     CDS             <1..488
                     /locus_tag="ACHOSM_RS20555"
                     /old_locus_tag="pgaptmp_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4560493.1"
                     /translation="GIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFME
                     SVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKHAIASYYHR
                     VARPRKMLCRGGGRIQKPSLATCRREVDEIINASLFMIYPVLDSAFKNRKRVEKIKHV
                     A"
     gene            579..653
                     /locus_tag="ACHOSM_RS20560"
                     /old_locus_tag="pgaptmp_004112"
     tRNA            579..653
                     /locus_tag="ACHOSM_RS20560"
                     /old_locus_tag="pgaptmp_004112"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asn,seq:gtt)
     gene            659..734
                     /locus_tag="ACHOSM_RS20565"
                     /old_locus_tag="pgaptmp_004113"
     tRNA            659..734
                     /locus_tag="ACHOSM_RS20565"
                     /old_locus_tag="pgaptmp_004113"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Thr,seq:tgt)
     gene            977..1300
                     /locus_tag="ACHOSM_RS20570"
                     /old_locus_tag="pgaptmp_004114"
     CDS             977..1300
                     /locus_tag="ACHOSM_RS20570"
                     /old_locus_tag="pgaptmp_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HGU4560494.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            1284..1760
                     /locus_tag="ACHOSM_RS20575"
                     /old_locus_tag="pgaptmp_004115"
     CDS             1284..1760
                     /locus_tag="ACHOSM_RS20575"
                     /old_locus_tag="pgaptmp_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGU4560495.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            1757..2224
                     /locus_tag="ACHOSM_RS20580"
                     /old_locus_tag="pgaptmp_004116"
     CDS             1757..2224
                     /locus_tag="ACHOSM_RS20580"
                     /old_locus_tag="pgaptmp_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4560496.1"
                     /translation="MNRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKKISEL
                     KQATATITDMQQRQRSADALDAKYTKELANAKAENDVLRRKLDNGGRVLVKGKCQVPA
                     STETSRTSGMGNDATVELSPVAGRNVLGVRDGIIRDQTALRTLQEYIRTQCLR"
     gene            2212..2364
                     /locus_tag="ACHOSM_RS20585"
                     /old_locus_tag="pgaptmp_004117"
     CDS             2212..2364
                     /locus_tag="ACHOSM_RS20585"
                     /old_locus_tag="pgaptmp_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560497.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSESRLNISLLMLDIEHLA
                     RGKGLR"
     gene            2443..2730
                     /locus_tag="ACHOSM_RS20590"
                     /old_locus_tag="pgaptmp_004118"
     CDS             2443..2730
                     /locus_tag="ACHOSM_RS20590"
                     /old_locus_tag="pgaptmp_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560498.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPQEWQEKMAQLLF
                     EYGDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
     gene            2956..3327
                     /locus_tag="ACHOSM_RS20595"
                     /old_locus_tag="pgaptmp_004119"
     CDS             2956..3327
                     /locus_tag="ACHOSM_RS20595"
                     /old_locus_tag="pgaptmp_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HGU4560499.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPDHIEGIIAQEAYFTAEDGAFGK
                     AIKAKHTGGEVNYQPHESLSLLTFCVLVLRNGFTVTGESACASPENFDAEIGRKIARQ
                     NAVNKIWMLEGYLLKQKLSEQ"
     gene            3431..3673
                     /locus_tag="ACHOSM_RS20600"
                     /old_locus_tag="pgaptmp_004120"
     CDS             3431..3673
                     /locus_tag="ACHOSM_RS20600"
                     /old_locus_tag="pgaptmp_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HGU4560500.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            3675..3854
                     /locus_tag="ACHOSM_RS20605"
                     /old_locus_tag="pgaptmp_004121"
     CDS             3675..3854
                     /locus_tag="ACHOSM_RS20605"
                     /old_locus_tag="pgaptmp_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560501.1"
                     /translation="MSRVINLGKEKKFPITQELYERLESVIHDYDGEISLCEAIGTLE
                     LLKQSLIEGGKESSA"
     gene            3878..4366
                     /locus_tag="ACHOSM_RS20610"
                     /old_locus_tag="pgaptmp_004122"
     CDS             3878..4366
                     /locus_tag="ACHOSM_RS20610"
                     /old_locus_tag="pgaptmp_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HGU4560502.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWADDNPL
                     WEGKVFSYQGKIIKANVPKMRAMTISGLCTFLDITRQTWGTFRSMEGFSDVTSRAEDI
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            4344..5840
                     /locus_tag="ACHOSM_RS20615"
                     /old_locus_tag="pgaptmp_004123"
     CDS             4344..5840
                     /locus_tag="ACHOSM_RS20615"
                     /old_locus_tag="pgaptmp_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HGU4560503.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPADGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENDEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVINAMDFGWDHPQAHIQLW
                     WDKDEDVIYLSRVWKAKQKKATEAWSAVKAWSKNTPTAWPHDGHQHEKGGGAQLKEQY
                     ADAGFDMLPDHATWPDGGNAVEPGIAEIRDMMLDGRFKVFNTCEPFFEEFRLYHRDEN
                     GKIVKLNDDILSAVRYGYMMRRFAIQMRDIKYPKEIDYSSYNIPCGVG"
     gene            5840..8041
                     /locus_tag="ACHOSM_RS20620"
                     /old_locus_tag="pgaptmp_004124"
     CDS             5840..8041
                     /locus_tag="ACHOSM_RS20620"
                     /old_locus_tag="pgaptmp_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGU4560504.1"
                     /translation="MADDRKMTDWHRKVLCNFDNAWSATQDMREQIIEAQRFVRVSGA
                     QWEGSTNAGYSFDEGRFEHYPRFELNKIARECDRIIGEYRQNRISVKFRPKDDKASEA
                     LAEKMNGKFRADYQETSGGEACDNAFDDAVTGGFGCFRMCADYEDEMDPSNEQRRISL
                     LPVYDPATCVFFDQDSKQYDRSDAMWAMEMFSMTPKAFEAEYPDSIAASLSRDDTGTQ
                     YDWSTPDAIYVGRYYEVRIEKVKLTAWRNPVSGETAIYDEEQIKDIVDELTDGAFELI
                     GERTVKKRRVYCGLLSGAEWLEEPKRIPGEHIPLIPVYGRRSFVDNQERIEGHAAKAM
                     DAQRLENLMVSMIADNATQAGGDGIPVVDVDMIPGPLATHWAERNKKRPAFLPMVSLK
                     NKNGDITAQAQVSSYTPPTQMPPALAGLLQYTGTAIQQITGASQLENMPSNVATDTVD
                     SIFNRMDTQSYIYMDNMAKSMRRAGVVWLSMAREVYGSDTPMRIVNDDGSDDVALMTG
                     EVVDRQTGQVIALNDLSQGNYEVTVDVGQSFATRRDATVKSLLSMLALIPPGTPKHDL
                     VSSMILDNMDGEGMDDLKEYNRNQLLLSGVIKPRTPEEQQMVEQAKQQQASQPDPAMV
                     AAQGQLLAGQAELQKAQNEQAAIQVKAFQAQTDAQVAAANVVKILVSADSQQKSDIRE
                     ALKLLGQFQQQQGDNARADAELVLKSQAQGHAQRMDISSILQKSTQQQPQQ"
     gene            8132..9025
                     /locus_tag="ACHOSM_RS20625"
                     /old_locus_tag="pgaptmp_004125"
     CDS             8132..9025
                     /locus_tag="ACHOSM_RS20625"
                     /old_locus_tag="pgaptmp_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HGU4560505.1"
                     /translation="MDQMAENTPEVEIETDASEQIPDDVELAEEVETEDGSESSGNDA
                     EEATETDDDESEQEFYFGDEKLDSPTSEDGAEHGLVKHLRKTIKEKDRELKELMRQSQ
                     KPVEQQPVITQPPRMPKLDDEDIGFDEEIYQQRMAKWAEDNGKYQQQEMARKQKEQEL
                     QAAYQERLSKYQQRVKALKVPGYQEAEQAVLEEIPIETQNAILFESEKPEIVVLALGR
                     NAELRKQLAEATNPVAIGRLLERIESKARIMPKAKTTAATTPTVKGSNGAVINNLDKL
                     KAKALETGDWTPYFAAKKAKK"
     gene            9044..>10167
                     /locus_tag="ACHOSM_RS20630"
                     /old_locus_tag="pgaptmp_004126"
     CDS             9044..>10167
                     /locus_tag="ACHOSM_RS20630"
                     /old_locus_tag="pgaptmp_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HGU4560506.1"
                     /translation="MANQLAKDLEIMFENYVEGFEVACVVSRNAKKFRPGDTAMQRAG
                     DVLYRPQHYHMNIEEGLDLSGKTPTALVQRLVPSVFKEPKNILYTLDAREMRDPEHKT
                     EAGRAAGMRLAAQIDSDLISMVTQRATNVITMADSTTGSQGRDLWNCAAGIDATMTAI
                     GVPQGINRRSFWNPFNYKDLAGELGHRAYAQGATLTAYEKAQIPPVASFDSYKTDISG
                     RVPKGTATSLTLAAEPAHKVEAKDANDMPVDNRQGTITVSASGLQVGDAFTIAGVNSV
                     HQITKDTTGQPQVFRVLAVSGTTVTISPKILPPDNADVASRPYANVDANAANGAAITI
                     LNKNAAPANLFWADGSVELMYGKLAFPTGQGPQVMTATTE"
BASE COUNT         2826 a         2422 c         2652 g         2267 t
ORIGIN      
        1 tgggcataag agaactaaac ctaaccaaag aacagcatga gtggctgaat ggctggcttg
       61 aactgtgggg cgcatgggtt tattcaggcc gcctggaaaa gcgcatgagc agcgtaatag
      121 cgaagttcat ggagagcgta gagccgggaa gagttatgac aaggccaatg tgtaatgatg
      181 atgatggaat gttgatttct caggtcgtcg attccgtcat gtacattgac aagaaagcct
      241 ttggcatcct cctcagctac tacgctcatg gatcttccaa gcatgccatt gcatcttact
      301 atcatcgtgt cgcaagacct cgcaagatgt tatgccgggg cggcgggcgt attcaaaaac
      361 catcgctcgc aacctgtcgc cgggaagttg acgaaatcat caatgcttcg ttgtttatga
      421 tttatccggt tctggatagt gcgtttaaaa accggaaacg tgtagagaaa attaaacatg
      481 tagcatagaa cgtgttgaca tcattgagca aatgagcaac actattcgca taagctgccg
      541 ttagtgactc ttaagttgca acggtggctt ttttatttgg gtcagtcgta taaaggtcat
      601 tacggaaggc tgttaacctt cttatcgtgg ttcgagtcca cgctgtcccg ccaaacatgc
      661 tggtttagct ccaatggtag agcagtcgcc ttgtaagcga atgggtagcg gttcaagtcc
      721 gttaaccagc accataactg agccgtagcc actggccatc ctgaattcat cagtgatagt
      781 tacgctgcgg ccttctacac atgaccttcg tgaaagcggg tggcaggagg ttgcgctaac
      841 aacctcacgc cgttttgccc gtgcatatcg gtcacgaaca aatctgatta ctaaacacag
      901 tagcctggat ttgttctatc agtaatcgac cttattccta attaaataga gcaaatcccc
      961 ttattggggg taagacatga agatgccaga aaaacatgac ctgttagccg ccattctcgc
     1021 ggcaaaggaa caaggcatcg gggcaatcct tgcgtttgca atggcgtacc ttcgcggcag
     1081 atataatggc ggtgcgttta caaaaacagt aatcgacgca acgatgtgcg ccattatcgc
     1141 ctggttcatt cgtgaccttc tcgacttcgc cggactaagt agcaatctcg cttatataac
     1201 gagcgtgttc atcggctaca tcggtactga ctcgattggt tcgcttatca aacgcttcgc
     1261 tgctaaaaaa gccggagtag aagatggtgg aaatcaataa tcaacgtaag gcgttcctcg
     1321 atatgctggc gtggtcagag ggaactgata acggacgtca aaaaaccaga aatcatggtt
     1381 atgacgtcat tgtaggcgga gagctattca ctgattactc cgatcaccct cgcaaacttg
     1441 tcacgctaaa cccaaaactc aaatcaacag cagccggacg ttaccagctt ctttcccgtt
     1501 ggtgggatgc ctaccgcaag cagcttggcc tgaaagactt ctctccgaaa agccaggacg
     1561 ctgtggcatt gcagcagatt aaagagcgtg gtgctttacc catgattgat cgcggtgata
     1621 tccgtcaggc tatcgaccgt tgcagcaata tctgggcttc actgccgggc gctggttatg
     1681 gtcagttcga gcataaggct gacagcctga ttgcaaaatt caaagaagcg ggcggaacgg
     1741 tcagagagat tgaggtatga acagagtaac cgcgattatc tccgctctgg ttatctgcat
     1801 catcgtctgc ctgtcatggg cggttaatca ttaccgtgat aacgccatga cctacaaaga
     1861 gcagcgagat aaaaaaatca gtgagctgaa gcaggcgacc gccaccatta ctgacatgca
     1921 gcagcgccag cgttctgctg atgcactcga tgctaaatac acgaaggagt tagctaatgc
     1981 gaaagctgaa aatgatgttc ttcggcgcaa gcttgataat ggtggcaggg tgctcgtcaa
     2041 aggaaagtgt caagtgccag cctcaaccga aacctcccgc acctccggca tgggcaatga
     2101 tgccaccgtc gaactctctc cagttgctgg acgaaacgtt ctcggtgtcc gggacggaat
     2161 tatccgcgac caaacagcac tgagaacgct tcaggaatac atcaggacgc aatgccttcg
     2221 atgatagcga taattttact catcatcctt cacatctggc tctgtagaca gggtggtgat
     2281 cacttctgga gtgaatccag attaaacatc tcattgctga tgcttgatat tgagcatctt
     2341 gcgcgcggta aggggctgcg ttgagataag agccagtcat tacaaatacc aggatttagc
     2401 ctcgcattcg cggggctttt tattgcaaca aaggtaaaga cgatggatga agaatatcgt
     2461 aaagacctgc aattgtggtt tggcctgacg catgcgtcgt tctgcgtgat gccgagagta
     2521 ttcatggagg ctatgccaca agaatggcaa gagaagatgg cgcagttgct ttttgagtat
     2581 ggcgacacga tcaaaacgga tgtctgcgga gttcacagct gtttcgttac tgccaaagac
     2641 ggcaacaacc gctttatgag gatgccagaa gatattctga actatcgtca tccccggcgt
     2701 gagttcatcg aatcatttct gaagaagtag ccattacaaa gcctatctgc gggtgggctt
     2761 gataatgaaa ccggagctta ttctgggcaa ccagttatgg cagtaccgcg aaacaaccca
     2821 agccagtaag tggggaaata acactggcag ccactgaaag atgaacctcc agccttatgg
     2881 caaaaaagat tctttgtggt ggcggactga tggaaagaca tcctaatcaa gcaaccactc
     2941 tacagggtca taattatgaa cgaccagcaa atcgaaaaag aaatcgttga gaaaggcaaa
     3001 acggcaccgc gcgttacgcc agaccatatc gaaggcatta ttgctcagga ggcatatttc
     3061 acagcagagg atggtgcctt tggcaaagcc ataaaagcga aacatactgg cggagaggta
     3121 aactaccagc cgcacgaatc actttctctg ctgacgttct gcgtcctggt gctgcgtaac
     3181 ggcttcaccg tcaccggaga gagtgcctgt gcaagtccgg aaaattttga tgcagaaatt
     3241 ggtcggaaga ttgcccggca gaatgctgta aacaaaatct ggatgctcga aggttacttg
     3301 ctgaagcaga agctaagcga acagtagtta ttacaaaagc cattccctac agagtggctt
     3361 tgataatggc ttatacccta cacgggataa cttaactgat atccctttta acggataaac
     3421 ggagccaaca atggcagaga ttattcccat gactgaagaa cagaaattcc agttagagat
     3481 ttacaaactg gtcatgaacc agaacgcagc cgcagaagaa gcatttcaat tcatcggcac
     3541 tgacgaactg aagcttgagc tattcaaaat tcacttccag tcaggcggcg ctaattcaga
     3601 tatcacgacc cgcacaatcg aagcggtgcg taaatcgaag gaagcgttag acctgttcac
     3661 taccggagca tgatgtgagc cgcgtaatca atttgggtaa ggagaagaaa ttcccaatta
     3721 ctcaagagct atacgagcgg cttgaaagcg tcatccatga ttacgatggt gaaatcagct
     3781 tatgcgaggc gattggcaca ctcgaattgc tgaagcagtc actgattgag ggcgggaaag
     3841 agtcctcagc ctgaaataac gattaagaga gatgaatatg gcagcaccaa agggcaaccg
     3901 attttgggag gcccgcagta gtcatgggcg aaaccctaaa ttcgaatcgc ctgaggcgct
     3961 gtgggctgct tgttgtgaat acttcgagtg ggctgatgat aacccgctat gggagggtaa
     4021 ggtattttca tatcagggaa aaataattaa ggctaatgtc cctaagatgc gagccatgac
     4081 tatttcagga ttgtgtacct tccttgatat caccaggcaa acatggggaa ccttccggtc
     4141 aatggaaggt ttttctgacg tcacatcacg agcggaagac atcatctacg accagaaatt
     4201 ctctggcgca gccgctgacc ttctcaacgc taacatcatc gcccgtgatt tgggcctcaa
     4261 agagcagtcg caagttgaag acgtgacacc tgataaggga gatcgcgata agcggcgctc
     4321 tcgtatcaag gagctattca accgtggaac tggacgcgat tcttgataac ctgagcgacg
     4381 aagagcaaat cgaattgctc gagctactcg aagaagaaga gaactaccgg aacacacacc
     4441 tgctatatga atttacgcca tacagcaaac agcgtgagtt catcgacgcc ggacatgact
     4501 atccagagcg atgttttatg gctggtaacc agcttggtaa gtcatttact ggtgctgctg
     4561 aagtcgcgtt tcaccttacc gggcgttatc ctggcacaaa aggctatcct gctgatggta
     4621 aatatggcgg tgagtggaag gggaagcgtt tctatgagcc tgttgtcttc tggattggtg
     4681 gcgagacaaa cgagactgta accaaaacga ctcaacgtat cctgtgcggt cgtatagaag
     4741 agaatgatga gcctggctac ggttccatac cgaaagaaga catcattagc tggaagaagt
     4801 ctcctttctt tccgaacctt gttgatcatc ttctggttaa gcatcacacg gctgatggcg
     4861 ttgaagatgg catttcaatc tgctacttca aaccatactc gcaaggccgc gctcgctggc
     4921 agggtgacac aatccacggc gtgtggtttg acgaagagcc accatacagc atttatggcg
     4981 aaggtcttac ccgtaccaac aaatacgggc aattctcaat tctgacgttt accccgctga
     5041 tggggatgtc tgacgttgtt accaagttcc tgaagaatcc cagcaagtct cagaaagtgg
     5101 tcaacatgac catctacgac gctgagcact ataccgacga gcagaaagag caaatcatcg
     5161 catcctatcc agagcatgag agagaggcgc gtgctcgcgg tattcctacg atgggtagcg
     5221 gccgaatctt ccagataccg gaagagacga ttaagtgtca gccgttcgag tgtcctgatc
     5281 acttctacgt catcaatgca atggacttcg gatgggatca cccacaggca cacatccagc
     5341 tttggtggga taaagacgag gacgtgattt atctttctcg cgtctggaag gccaaacaga
     5401 agaaggcgac agaggcatgg agtgctgtta aagcatggag caaaaacacc cctacggctt
     5461 ggcctcatga cgggcatcag cacgaaaagg gaggcggcgc tcagctcaag gaacaatacg
     5521 ccgacgctgg gttcgacatg ttgccagatc atgcaacatg gcctgatgga ggtaatgcgg
     5581 tcgaacccgg gatagcagag atacgcgaca tgatgctcga cggtcgtttc aaggtattta
     5641 acacctgcga gccattcttt gaagagtttc gcctgtatca ccgcgatgag aacgggaaga
     5701 tcgtcaagct aaatgacgac atcctttctg ctgttcgcta tggctacatg atgaggcgtt
     5761 ttgcaataca gatgcgagac atcaaatatc ctaaagagat tgattactca agctacaaca
     5821 taccttgcgg agttggatga tggctgatga tagaaagatg actgactggc atcgcaaggt
     5881 gctgtgcaac tttgataatg cctggtcagc aacgcaggat atgcgtgagc agattattga
     5941 ggcccaacgt ttcgtccggg tatccggcgc acagtgggaa ggcagcacaa acgctggtta
     6001 ctcatttgat gaaggcaggt ttgagcatta tccgcgcttt gaactgaata agattgcccg
     6061 tgaatgtgat cgcatcattg gcgagtatcg acagaatcgc atcagcgtta aattcaggcc
     6121 gaaggatgac aaggcatcgg aagcgttagc cgaaaaaatg aacggcaaat tccgcgctga
     6181 ctatcaggaa acatccggtg gcgaagcgtg tgataacgca tttgatgatg ctgtaacggg
     6241 cggattcggt tgtttccgca tgtgtgccga ttacgaagat gaaatggatc cgagtaacga
     6301 gcagcgacgc atcagccttc tccctgttta cgacccagcg acatgcgtct tcttcgatca
     6361 ggacagcaaa caatatgacc gctctgatgc tatgtgggct atggaaatgt tctccatgac
     6421 gcctaaagcg ttcgaggctg aataccctga ttccattgcg gcaagccttt ctcgggatga
     6481 cactggtact cagtatgact ggtcaacgcc tgacgccatc tatgttggac gttactacga
     6541 agttcgcata gagaaggtga agctcacggc gtggcgcaac cctgttagcg gagaaacggc
     6601 aatctatgat gaagagcaaa tcaaagatat tgtcgacgag ctgaccgatg gtgcattcga
     6661 actgattggc gagcgaacgg tgaagaagcg ccgcgtttat tgcggtcttc tgtctggcgc
     6721 tgaatggctg gaagaaccga agcgtattcc gggtgaacat attcctctca tcccggtata
     6781 tgggcgtcgc tcatttgttg ataatcagga gcgaatcgaa ggccacgcag caaaagcgat
     6841 ggatgcacag cgtcttgaga acctgatggt ttccatgatt gcagataacg ctactcaggc
     6901 tggcggtgat ggcattcctg tagttgatgt tgacatgatt cctggtcctc tcgccactca
     6961 ttgggcggag cgcaacaaaa agcgcccggc gttcctgccg atggtcagtc tgaaaaacaa
     7021 aaacggagat attactgcgc aggctcaggt cagcagttat acgcctccga cacaaatgcc
     7081 tccagctctt gccgggctat tgcagtacac cggaacggct attcagcaaa ttacaggtgc
     7141 gtcgcagctt gagaacatgc cgagcaacgt cgccaccgat accgttgata gcatctttaa
     7201 caggatggac acgcagtcat atatctacat ggataacatg gctaaatcca tgcgccgtgc
     7261 tggcgtcgtg tggctttcta tggctcgtga agtctatggc agcgatacgc caatgcgcat
     7321 cgttaatgat gacggcagcg atgacgtggc gctgatgact ggtgaagtgg ttgaccgtca
     7381 gacagggcag gttatcgcgc ttaacgacct ttcgcagggt aactatgaag tgactgtcga
     7441 tgtcggtcag tcgttcgcta ctcgccgtga cgcaacggtt aagtcgttac tttccatgct
     7501 ggcacttatc ccaccaggaa cgccgaagca cgaccttgta tcgtcgatga ttctcgacaa
     7561 tatggacggc gaagggatgg acgaccttaa agaatacaac cgcaatcagt tgcttctgtc
     7621 tggagttatc aagccgagaa caccagaaga acagcagatg gttgagcagg cgaaacaaca
     7681 acaggccagt cagccagatc cggctatggt tgcagcgcaa ggtcagcttc ttgccggtca
     7741 ggctgaattg cagaaagcgc agaacgaaca ggcagccatt caggttaaag cattccaggc
     7801 acagactgat gctcaggttg cagcggcaaa cgttgtgaaa atcctcgtat ctgccgatag
     7861 ccagcagaaa tctgatatcc gcgaggctct gaaactgctt ggacagttcc agcaacagca
     7921 aggagacaat gcccgtgctg atgcagagct tgtcctgaaa agtcaggcac agggtcatgc
     7981 gcagcgcatg gacatcagca gcatcctgca aaaatcaact cagcaacaac cacagcagta
     8041 attaacccat aacgtgcaat ggctgtcttt atgaggcctg gcaccctatt gccttccgat
     8101 gggctgaaca tcgagtaaac aggggtaaca aatggaccag atggcagaaa acacaccaga
     8161 agttgaaatc gaaaccgacg cgtcagagca gattcctgat gatgtcgaac tggctgaaga
     8221 agtcgaaaca gaagatggca gtgagtcctc cggcaatgat gcagaggaag ctactgaaac
     8281 tgatgacgac gaatcagaac aggaattcta ctttggtgac gaaaagctgg attcgccaac
     8341 cagcgaagat ggcgcagagc atggactggt aaaacacctg cgcaagacga ttaaagagaa
     8401 agaccgcgag ctgaaagagc tgatgcgtca gtctcagaaa cccgtcgagc agcagccggt
     8461 aatcactcaa ccaccgcgaa tgccaaaact ggatgatgag gacatcggtt tcgatgaaga
     8521 aatctaccag caacgcatgg ctaagtgggc agaggataac ggcaagtacc agcaacagga
     8581 gatggctcgc aagcagaagg agcaggagct tcaggctgcc tatcaagagc gattatccaa
     8641 atatcagcaa cgtgttaagg ctctcaaagt tcctggctat caggaagcag aacaggccgt
     8701 actcgaggaa atccccatcg agacacaaaa cgcgatcctg tttgagtcag agaagccgga
     8761 aatcgttgtt ctggcactcg gtcgcaacgc tgaactgcgc aagcaactgg cagaagctac
     8821 caaccccgta gcaattggtc gtctgctgga acgtatcgaa tcgaaggcca gaatcatgcc
     8881 aaaagcaaaa accacggcag ccacaacccc gacagttaag gggagcaacg gcgcagtaat
     8941 caataacctc gacaaactga aagccaaggc gctggaaact ggtgactgga cgccgtattt
     9001 cgccgctaaa aaggcaaaaa aataacctat cggagcatta agcatggcta accaattagc
     9061 aaaagacctt gaaatcatgt tcgaaaacta cgttgaaggc tttgaggtcg cctgcgtagt
     9121 ttcccgtaac gctaaaaaat tccgtcccgg tgatacagca atgcagcgag caggtgatgt
     9181 tctgtatcgt ccgcagcatt accacatgaa tattgaggaa ggcctagacc tcagcggcaa
     9241 aacgccaaca gcactggttc agcgccttgt tccttctgtg ttcaaggagc cgaaaaacat
     9301 tctgtacact ctggatgcgc gtgaaatgcg tgacccggaa cataaaactg aagctggtcg
     9361 cgccgcaggt atgcgccttg ctgcacagat tgactctgac ctgatttcca tggttacgca
     9421 gcgtgctact aacgtgatca cgatggctga ctcaaccaca ggttcacagg gccgtgattt
     9481 gtggaactgt gcggcaggta ttgatgccac catgacggcg attggtgtac cgcagggtat
     9541 caaccgtcgc tctttctgga accccttcaa ctacaaagac cttgctggcg agcttggtca
     9601 ccgtgcctat gctcagggcg caacactgac agcatacgaa aaagcgcaaa tccctccggt
     9661 tgcgtccttc gatagctaca agaccgatat ttctggtcgt gttccgaagg gtacagcaac
     9721 ttccctgacg ctggcggctg aacctgcgca caaggttgaa gcgaaagatg ccaacgatat
     9781 gccagtggat aaccgacagg ggaccattac ggtatctgca tctggtttgc aggttggcga
     9841 tgcgttcacc attgctggcg tgaattccgt acaccagatc accaaagata ccaccgggca
     9901 gccgcaggta ttccgcgttc tggcagtaag cggaacgaca gtaactatct ccccgaaaat
     9961 tctgccgcct gacaacgcgg atgtcgccag ccgaccatat gcaaacgttg atgctaacgc
    10021 ggcaaatggt gcagcaatta ccattctcaa caaaaatgcc gcaccggcta acctgttctg
    10081 ggctgatggt tctgttgagc tgatgtacgg caaactggcg ttcccgactg gtcagggtcc
    10141 acaggtaatg acagcaacca ccgagca
//
LOCUS       DAZKHC020000131        10129 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZKHC020000131 DAZKHC020000000
VERSION     DAZKHC020000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.131"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(179..733)
                     /locus_tag="ACHOSM_RS20635"
                     /old_locus_tag="pgaptmp_004127"
     CDS             complement(179..733)
                     /locus_tag="ACHOSM_RS20635"
                     /old_locus_tag="pgaptmp_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGU4560507.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIDKKVIAF"
     gene            complement(742..1101)
                     /locus_tag="ACHOSM_RS20640"
                     /old_locus_tag="pgaptmp_004128"
     CDS             complement(742..1101)
                     /locus_tag="ACHOSM_RS20640"
                     /old_locus_tag="pgaptmp_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4560508.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     VVSGGRLGVKIYKIESE"
     gene            complement(1114..2163)
                     /locus_tag="ACHOSM_RS20645"
                     /old_locus_tag="pgaptmp_004129"
     CDS             complement(1114..2163)
                     /locus_tag="ACHOSM_RS20645"
                     /old_locus_tag="pgaptmp_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4560509.1"
                     /translation="MRVLLRPVLVPELGLVVLRPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAVCNQVADALPEAVLRRSLGLRAEKIRSLYRESDIVPGEQTAISILKQR
                     TKNLAPLPHTHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(2165..2443)
                     /locus_tag="ACHOSM_RS20650"
                     /old_locus_tag="pgaptmp_004130"
     CDS             complement(2165..2443)
                     /locus_tag="ACHOSM_RS20650"
                     /old_locus_tag="pgaptmp_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560510.1"
                     /translation="MAQNSRSHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(2510..2773)
                     /locus_tag="ACHOSM_RS20655"
                     /old_locus_tag="pgaptmp_004131"
     CDS             complement(2510..2773)
                     /locus_tag="ACHOSM_RS20655"
                     /old_locus_tag="pgaptmp_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560511.1"
                     /translation="MMKIEELREIFSEDGLYTVRVENGAIVSHCRIKCLQSLHRKSGA
                     ALIYFVDGLVTDGFILRENEFVTSLQSLKEAGLKAGFSAFEDE"
     gene            complement(2928..4031)
                     /locus_tag="ACHOSM_RS20660"
                     /old_locus_tag="pgaptmp_004132"
     CDS             complement(2928..4031)
                     /locus_tag="ACHOSM_RS20660"
                     /old_locus_tag="pgaptmp_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4560512.1"
                     /translation="MEHQVNNFTEYRGDKLKLARMAVGLSCEELAEKIGKTKQFVSKL
                     EKGCRPSEQCLELISSALMIKSSFLFTERKYALESDVCHFRSKKSRTQTLTNSVLARA
                     EILNIIISAVEGEIEFPDVNIPEHPGAELLTPNDIERVAEDCRRAWNLGLGPISSMVK
                     LAESLGVIVAHVTGVDDRVDAFTVHNNRPVIIRNNVKKSICRFRSDLGHELGHLVMHE
                     GITTGDKLTESQADHFSSALLVPRLSFIKEFPRIRGKQFDWNALVEFKLRWKISLKMC
                     IYRASALGLLTQEQARTGYMHLNSRGYTRVEPGDELLRPEEPGMLAEAIEMLDDATWL
                     RILMKTGLSQDLIRELFSINRPITNPRNIFQIV"
     gene            complement(4012..4662)
                     /locus_tag="ACHOSM_RS20665"
                     /old_locus_tag="pgaptmp_004133"
     CDS             complement(4012..4662)
                     /locus_tag="ACHOSM_RS20665"
                     /old_locus_tag="pgaptmp_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560513.1"
                     /translation="MNENQLAPCWEFQPYLAENYVRHLLAEIANVLEQLYYHKHALDS
                     NWSEGVRAYDWVRNHLIQNEDAIPGLEMISKGLDYVVALNKVPLQFTKDCINNPKKKH
                     RLRRNKVEYEQLSLFGDVEAEQDITWRVIAEPFLSEEGDGELESTLPRWEVALVGFNT
                     YGAQISIVSHQSTASMPLMPLDYNTLPDEAEINKVPLRRRTKDKDLDVSSDGTSGE"
     gene            complement(4828..4983)
                     /gene="hokD"
                     /locus_tag="ACHOSM_RS20670"
                     /old_locus_tag="pgaptmp_004134"
     CDS             complement(4828..4983)
                     /gene="hokD"
                     /locus_tag="ACHOSM_RS20670"
                     /old_locus_tag="pgaptmp_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU4560514.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(5355..6314)
                     /locus_tag="ACHOSM_RS20675"
                     /old_locus_tag="pgaptmp_004135"
     CDS             complement(5355..6314)
                     /locus_tag="ACHOSM_RS20675"
                     /old_locus_tag="pgaptmp_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HGU4560515.1"
                     /translation="MARKLIIFGNGLGMALDPAHFSLDRALEEIWHRPNFLKDIHKQL
                     IERCLQRQGPPEGEHELDTLHQAVTYCKALAQIGEGNVHWLTDDGLNFPKITATYIHK
                     VATRLHNYDKGLPQRFENALVEFVKNTHSHIATLNYDKLLYNSFIDNDIFNGYDGVLV
                     DGMLSHGFSSAALERKYNRRFGYYLHLHGSPLFINQHENVLKLSRSQLTLDIDEPSEH
                     IVLTHIKRKPSVIAASNVLSTYWDYLQFALFESEEIILFGYSGLDIHLNLLIRPYLTS
                     KPLRVIEWSGAGEQNAREIYWKNQLRREVTVIRLDNITDFIDW"
     gene            complement(6755..7162)
                     /locus_tag="ACHOSM_RS20680"
                     /old_locus_tag="pgaptmp_004136"
     CDS             complement(6755..7162)
                     /locus_tag="ACHOSM_RS20680"
                     /old_locus_tag="pgaptmp_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU4560516.1"
                     /translation="MAKVFTKEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYHSGYGVFPSERARKDWQNARKKLSRAKVKKPVVVDPGLIWTLPDG
                     EIRRYDRRMNIICRECRKSEVMQRVLAFYQGYA"
     gene            complement(7203..8179)
                     /locus_tag="ACHOSM_RS20685"
                     /old_locus_tag="pgaptmp_004137"
                     /pseudo
     CDS             complement(7203..8179)
                     /locus_tag="ACHOSM_RS20685"
                     /old_locus_tag="pgaptmp_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8160..8681)
                     /locus_tag="ACHOSM_RS20690"
                     /old_locus_tag="pgaptmp_004138"
     CDS             complement(8160..8681)
                     /locus_tag="ACHOSM_RS20690"
                     /old_locus_tag="pgaptmp_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4560517.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKREKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPSVVERETDELIEKAIAFKHAYRQQY
                     QQKAGWNYEPSFC"
     gene            complement(8665..8892)
                     /locus_tag="ACHOSM_RS20695"
                     /old_locus_tag="pgaptmp_004139"
     CDS             complement(8665..8892)
                     /locus_tag="ACHOSM_RS20695"
                     /old_locus_tag="pgaptmp_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU4560518.1"
                     /translation="MLKVDAITFFGSKTKLANAAGVKLASVAAWGELVPEGRVMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHP"
     gene            8973..9380
                     /locus_tag="ACHOSM_RS20700"
                     /old_locus_tag="pgaptmp_004140"
     CDS             8973..9380
                     /locus_tag="ACHOSM_RS20700"
                     /old_locus_tag="pgaptmp_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4560519.1"
                     /translation="MDTRTLGQRVLARRKELRLTQREAARLAGVAHVTISQWERDETQ
                     PVGKRLFALADALKCSPTWLMFGDEDKAPVPAQELHVETELTPSHKELIELFDALPSS
                     EQEALLSEMRARVENFNKLFEEMLKARKNKSIK"
     gene            9549..9701
                     /locus_tag="ACHOSM_RS20705"
                     /old_locus_tag="pgaptmp_004141"
     CDS             9549..9701
                     /locus_tag="ACHOSM_RS20705"
                     /old_locus_tag="pgaptmp_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4560520.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTLKTESGARRW
                     LARNTD"
     gene            9713..10087
                     /locus_tag="ACHOSM_RS20710"
                     /old_locus_tag="pgaptmp_004142"
     CDS             9713..10087
                     /locus_tag="ACHOSM_RS20710"
                     /old_locus_tag="pgaptmp_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560521.1"
                     /translation="MEFKDLPIPFQEMAANIVRSQLATPDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKVESTYKISINH"
BASE COUNT         2714 a         2566 c         2073 g         2776 t
ORIGIN      
        1 ccaaaaagcc agcaagcaga aagaccggta ttcgcaacca ccagcgcatt taacgccccg
       61 tgccgttttt cagtcataaa aaaacccgca aaaagcgggc tctttcaaat gtccatgtct
      121 gctattcgcc tcgcggtata gctttgcgaa gcgtaccgga attgaagcag tttttacgtc
      181 aaaaagcaat aactttttta tctataccaa aagccataac cattggtttg tacaaaataa
      241 attctgccac ctttagccaa tgctcaatgc gtctttcaca ggttcttaaa ctccattccg
      301 gatgtgcatc attcagcagt tcagccattt tgcgcttagt catcccccgc cccacataac
      361 gctgactcag aacattgagc agcccgggat aacctgccag tacttcacca ataaccctgt
      421 cgattattaa cgcctctgaa tcggtacaat gtgccagcca gcttttttga ttgccgttga
      481 tcatatcccg caaaaaagcc tcaagttcag gtttgtccag acccgctttt ttcatcctcc
      541 ggagcgcctc gttaattgcc gtttttgtca gctttttaga ggtcagtaat tggttgaaca
      601 tatttcccgt cttaccgtcg ccaatatacg accaacgccc ccacatacgc agtttcccct
      661 ggatccagac actttccagc gttttcaggc gtaaatgctc accgcttttg cctgtaattt
      721 ccgggtatat catatttatg ctcactcact ttcaattttg taaatcttca cgcccagccg
      781 cccaccagaa acgacctgac cacgcacaat attgatttca tcaaactgct cgtcgtcgat
      841 aagcaacccc gcatgtgtca gtgcatccag tggtgctttc agaatattgt ccaggtcacg
      901 acggcgctta tccggtggct ctgcaatcac ctttatcgcc agccttcctg acaggcttaa
      961 tttcagccgc tgctggcgaa caataagtgc cacatcccgg cgataacgct caccggcttt
     1021 tgatacaaaa tatgtgctgc cacgacgtcg ccagtaggtg ttcaccgtcg gcgggtaagg
     1081 caaaacaaac tctatacgca tcagtaacct cttttacccg agcacgccgg ttgcaaaagc
     1141 gtgatcaaga aaacgaaaaa ttaaatcaac ctgggaacca tgcttttctt cgaacgccag
     1201 cggatccgca tgaagttcgt tgtgatgctc ccgacacagc ggtagcgtga aaatatcgtg
     1261 ggattttgtt cccattccac cctgaccatg accaatcaga tgatgaggat cgtccgctgg
     1321 cttaccacaa cacgcacacg gctgtgtctt tacccagcgt gtgtatttct catttaccca
     1381 gcggcgacgt ttaggtcgtt tcatgaagga ttccggagac tccggatcaa cggcaatgct
     1441 gaccaccgtc ttttcctgtg gtgggttctg ttgctggtgg gtgtgaggca gcggcgcaag
     1501 attttttgtg cgctgtttca gtatgctgat ggcggtctgc tctcccggta cgatgtcgct
     1561 ttcgcggtac aaggagcgga ttttttccgc acgtaacccc agagaacgac gtaatactgc
     1621 ctccggaagc gcgtccgcca cctgattgca gaccgcccac caggataatt cagccagcga
     1681 caattcccgc tcctgcgtgc cattcattgc atggcgtatg acgtcaatca tccatgctga
     1741 caggttttga tgagcaagtt gcccgagtga ttcggatgtc tggtcacgca gctggttgtc
     1801 gcagtgccag cacaacacca ttgcgccggt accataacgg tgaatgacgg tttcactatg
     1861 gtgataatcg ccgtgtggcc actggcagga tttaatatgg cgcaacagcc agtcagacaa
     1921 tgcaccagca ccaccagcag cacgaatcac ccgtgcgtta ctgaaaaacg gcagcaatgt
     1981 tttgtcttcc accagcggct ggcgaacggc agggacgact ccggacggca gaccgcgcat
     2041 gcttttcggt tcaggctcca ccagcactcg agggttatga aatacttgca tggattcacg
     2101 gcccggccta aggaccacca gcccgagttc cggtaccaga accggtcgaa gtaatacccg
     2161 cacgttacct ccagatgcgt tgctggaatg tgcgggacgg acgcggtggg cgttcggaat
     2221 aaggaagcct gacagagatt atccagtgac gataatcgag actgagggct ttcttaacct
     2281 cgtatccgcg cctgcggtaa cactgaatca gccattcggc ctgttcttca gtgcatgggg
     2341 gatgctggta ccaatcagat ttaaatgcgt gagagcgccg cccgtgcctg ctggcaaaga
     2401 cggctgaatt atcagaattg tgtgatctgg aattttgcgc catcggcttt ctccggtggc
     2461 acagtgttac tcaacagggg ttcagtcctg cgctgaattg tagatgaatt cactcatctt
     2521 caaaagcaga aaaaccagcc ttaagcccag cttctttcag agactgcaat gatgtgacaa
     2581 attcattttc acgcaaaata aaaccatccg tcacaagtcc atccacaaag taaattaacg
     2641 cagctccact cttcctatgt agagactgta aacatttaat acggcagtgg ctgacaatag
     2701 cgccattctc aacgcgcaca gtatagaggc catcttcact aaaaatttca cgtaattctt
     2761 cgattttcat caacagaatc cttccagata aatagcactc cccctgttcg gggtccatcc
     2821 ctcttctccc tgcgcgctac ttaagtattt ttgattctat tctggcaccg tccaaaactt
     2881 caaacacgtt gaaaataaaa acaaaaaacc cgccgaagcg ggtatactca aacaatctgg
     2941 aaaatatttc ttggatttgt aataggtctg ttgatggaga acaactcacg aattaaatct
     3001 tggctcaagc cagttttcat aagaattctt agccaggttg catcatccag catttcaatc
     3061 gcctcggcca gcatgccggg ttcttcaggg cgcaaaagtt catcaccagg ttcaactctc
     3121 gtataccctc tggaattaag atgcatatag ccagttcttg cctgttcctg ggtcaataag
     3181 cctaatgcgc tggctcgata aatacacatt ttaaggctga ttttccatct aagtttaaat
     3241 tcaaccagag cattccagtc gaattgctta cctcgtattc gtggaaattc tttaatgaaa
     3301 gataacctgg gaactaataa ggcgctcgaa aagtgatcgg cttgtgattc cgtaagttta
     3361 tcacctgtcg ttatgccctc atgcattact aaatgcccta attcatgacc taaatcagag
     3421 cgaaatctac atatgctttt tttaacattg ttcctgatga taacaggcct gttattgtga
     3481 acagtaaaag catcaacacg atcatcgact cccgtaacat gcgcaacgat tacccctaaa
     3541 ctctccgcca atttaaccat tgatgatata gggccaagac ctaaattcca ggcacggcga
     3601 caatcttctg ccactcgctc aatatcattc ggagtaagta attcagcccc tgggtgctcc
     3661 ggtatgttaa cgtcaggaaa ttcgatttca ccttcaacag cagaaattat aatattaaga
     3721 atctcagccc tggccaatac actattagtc agcgtttgag tcctggactt cttactccga
     3781 aaatggcaga catcactttc cagagcgtat tttcgttcag taaaaagaaa actggactta
     3841 atcataagcg ctgaagatat taactcaaga cattgctccg atggcctgca ccccttctcc
     3901 agtttgctaa cgaattgctt tgtcttgcca attttttcgg ctaactcttc acaagaaagc
     3961 ccaacagcca ttctcgctag tttgagctta tcaccccgat actcagtgaa gttattcacc
     4021 tgatgttcca tcactgctca catccaaatc tttatccttc gtacgccgac gaagaggcac
     4081 cttattaatc tccgcttcgt cagggagtgt gttataatca agaggcataa gcggcatcga
     4141 tgctgtagat tgatgagaaa ctatactaat ctgagcacca taagtattaa atccaacaag
     4201 agctacctcc caacgaggca gtgtggactc taattcacca tcgccctctt cggataaaaa
     4261 aggctcagct atgactcgcc atgtaatatc ttgctcagcc tcaacatcac caaacaatga
     4321 gagctgctca tactctactt tatttcgacg cagacgatgt ttctttttgg ggttattaat
     4381 gcaatctttg gtaaattgta gcggaacttt atttaaagca actacatagt ccaacccctt
     4441 ggaaatcatc tcaaggccag gaattgcatc ttcattttga ataagatgat ttctgaccca
     4501 atcataagcc cttacacctt cagaccagtt gctgtctaat gcgtgcttat gatagtacag
     4561 ctgctcaagt acgttagcga tctccgccaa caagtggcga acatagtttt cagcaagata
     4621 aggttgaaat tcccaacaag gagctaactg attttcattc atttcaagtt tcgctttttt
     4681 tagaattcgt aaaccacata ttttcgcatt tttctatttt tgtcaaccag actaatgcaa
     4741 aaacccgccg aagcgggtta agtgcgggtg cgttgaggat gcctggcaca tcagaggtgg
     4801 cgggagatta ctcccccgcc gggtctctta ctcctcaggt tcgtaagctg tgaagacagc
     4861 gacctccgtc tggccggttc ggattcgtac ctcgcagagg tctttcctcg ttaccagtgc
     4921 cgtcactatg acggttaaac agatgacgat cagggcgatt aacatcgcct tttgctgctt
     4981 catagcctgc ttctccttga ccttttggtc cgtaagaggc taatctctat gtgtcgcata
     5041 gatatggcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
     5101 tctctatctg ccttttggtg ttcatgcctg aggcagatag cctcaagcac ccgcagcaat
     5161 tctacttaac tcccgtcacc tcgccaatat aaaatcaatc agaaaggcga tccataagaa
     5221 caatagcaag acaataaatt gccactacag ccgcaatagc cagcgcgcat ttgagaacca
     5281 gcacgataac ctcctgtatt ggacgtacac cagctctgat aaatatgagg ctgcctcttt
     5341 aacttcgcat tgcgctacca atcaataaaa tcagtgatgt tatccaacct aattacagta
     5401 acttcccgtc tcaactggtt tttccaatat atctcacgag cgttttgttc accagcaccg
     5461 ctccactcaa ttactcttaa aggcttagag gttaggtaag gtcgaataag caggttaagg
     5521 tgaatatcga gaccagagta accaaacaat attatttctt ctgattcaaa taatgcaaac
     5581 tgcaaataat cccagtacgt agaaagaaca ttagatgctg ctataactga cggttttctt
     5641 tttatatgag ttaacactat atgctcacta ggttcatcta tgtcaagagt aagttgtgag
     5701 cgtgaaagtt ttaaaacatt ttcatgctga ttaataaata gtggtgaacc atgcaagtgc
     5761 aaataataac caaacctacg attgtatttc cgttcgagcg ctgcagagga aaatccgtgg
     5821 cttagcatcc catcaactaa aaccccatca tacccgttga atatatcatt atctatgaat
     5881 gaattgtata ataatttgtc atagtttaat gtcgcgatat gtgagtgtgt gttctttaca
     5941 aactccacaa gcgcattttc gaatctttgt ggcaaacctt tatcgtaatt atgcaacctg
     6001 gttgcaactt tgtgaatata agtggcggta attttgggga agtttagtcc atcgtcagta
     6061 agccaatgca cgttcccctc tccaatttga gcaagagctt tacaatatgt gacagcctgg
     6121 tgaagggtat ccaactcatg ttctccttct ggtggacctt gccgctgaag acaacgctcg
     6181 ataagttgct tgtggatatc ttttaaaaaa ttggggcgat gccaaatttc ttccaacgct
     6241 ctgtctaagg agaagtgagc aggatctaaa gccataccga gaccgttgcc gaatattatt
     6301 aattttcttg ccatataggg ggactcatat tggaataacg ctggatagcg tcgcgcgata
     6361 cgcttaagtt taggattaat agacattccg tttgattaca cttgttatta caaacttgca
     6421 tgcaaagaag cccaagctgt taggtaaaca gagaccgcag tggcaagata atctagatat
     6481 agcctataaa atccaacaac aaatagcggc ctgttttcta tgacttttct tttccctaaa
     6541 tatcgcttat ctaataaaat gcaataatgt attgcacagt atttaatgtg tattaatata
     6601 gataagtatt ggtcttattt gataacaaat cctgagcctc aaaatgtctg cttttcacac
     6661 aaaaccaaca agtaggctat ttttagctct gtgccgcgaa aatgtcaatt catatccgaa
     6721 ccaatactct ttaatttcat tacctgtgac aattctaggc atatccctga tagaacgcca
     6781 atacacgctg cataacttcg ctcttccggc actcgcgaca gattatgttc atacgcctgt
     6841 cgtagcggcg tatttctccg tcgggtaatg tccagataag gcccggatca accacaacag
     6901 gtttcttcac ctttgccctc gagagttttt tgcgggcgtt ttgccagtcc ttacgagccc
     6961 gttctgacgg gaacacccca tagccagagt gatatacatc accactggca accagctctc
     7021 tggcaagaac gctcatcaga tatcttgtcg cacctgtttt agcttccagt tgccgtaacg
     7081 tctcacgacc gctctggcgc acgagttcca ccacctgtcc tttaattttt tcccgctctt
     7141 cctttgtaaa tacttttgcc ataagtcctc ctgaacatta ctttatgccc taaaatcacc
     7201 acttatcccc tgaaaccagg cggaatttcg gtatccggtt cagaaatatg attcacacaa
     7261 cactgtacag gtgaacgccc caggcggatg accaggtcgt cccatttttc gcgaagcttt
     7321 gacgggctca tgatgttttt acccagaatg gatctcgctg tacccgacca aacatttcgc
     7381 aaatttgtct gtggcttctg ccatccagca tccgcattgt gcgcacgtca ttggcccatg
     7441 cggtccagtt tggttctttc ggtcgcgtga tctcgccatc atcgctggca gcctgctcgt
     7501 aaagactcac gattcgtccc cagatccact gtgcgcacgc caaatcttcc tgacttcccc
     7561 actggcgttt tttcgcactg aacacaaccg cgtcagggtg tcgggttaaa aaatcctgtt
     7621 cagccgtctg cgggtccggt tgcgaagctt ccggacgaga agtgttttta ttctctgtag
     7681 taatctctgt tgtattctct gttgtattct ctgtaagatc atcaggccat tttgacccga
     7741 tgacattggg tcgttttgaa ccaatggagc gtgccatttt gacctcttcc atcgtgtcat
     7801 tttgacctga tggagcggcg cattttgacc tgatggattc gctcactttg ccaccatcta
     7861 aaagctcact ctcgtaatta atcgtgtaaa aattagtcat atcacgcttt gatttgttga
     7921 gcttttcgca acgcaaaagc cccagcgttt tcagacttgc aaatgcgcgt tttaacgttg
     7981 actctgacca gaacgggaac tgttccagcc attgttctgt tgtgttataa atccagcgaa
     8041 ccccgtcaca ttctataccg gaactggtat ctctcaacca gtaatgcaac tgctgcaaca
     8101 caatggcttc atttaagcca atcttcatcg caagctgtgt gtttataacc agtgggcgtt
     8161 cagcaaaaag aaggctcata attccatcca gctttttgtt ggtattgctg acgatacgca
     8221 tgcttgaaag caatagcttt ttctataagc tcgtcagtct cacgttccac aacagatgga
     8281 tccgcaaaaa gcagcccgga ctccaccaca tcaccatatt ctttgtttaa cccggcgatc
     8341 atgtacgtaa tgctttttcc gtcagtaatt tcacaataca acctgaaatc gctgatccgg
     8401 atagcctcca taattgccgg aatcagcgcc gtgaattttt cccgcttatc cctggtgtcg
     8461 atagctttcc agcgttcgaa tatcttcacc cggttaacgc ccaacgcccg ttgatcaacc
     8521 tcgccatcat taaacgtgac gcgttgaaca tcgatgttcg ggcgttcttt cagagcccag
     8581 aatgcttccg tgattaatat cgtcgcctgc tcctgtgtca ttcctggtcg gcatacccag
     8641 gcatccagag cctcacaaac ctgttcaggg gtgattttca ttgttcaacc gccccgcccg
     8701 ctttgcctta cgatattcgt cataaacttt ggggtcgtac tgaagttccc cgccggatgc
     8761 ctcttgcagg cgcatcacgc gaccttcagg aaccagttcc ccccatgcag caacacttgc
     8821 cagtttcact cctgcggcat tggcaagttt tgttttgctg ccaaaaaaag taattgcgtc
     8881 aactttaagc atcaaagccc cctcttgtta gatattccta acggtaatgt gcgcgggata
     8941 cctaagtcaa gaaaaattag aattacctaa ctatggatac aagaacccta ggccagcgag
     9001 ttctggcacg acgaaaagaa ttacgcctaa cacaacgaga agctgcacgc ctcgctgggg
     9061 tagctcacgt cacaatttca caatgggaaa gagacgaaac ccaaccggtc gggaagcggt
     9121 tatttgcttt agctgatgcg ctgaagtgct cgcctacatg gctaatgttt ggtgacgaag
     9181 acaaggcacc agtgccagca caagaacttc atgtggaaac cgagttaact cccagccaca
     9241 aagaattgat cgaattattc gatgctcttc cctcctccga gcaggaagcc ttactgtctg
     9301 aaatgcgcgc aagagtagag aatttcaata aactcttcga agaaatgctt aaagcacgta
     9361 aaaacaaatc aataaaataa cactcctttc agttacttag attccctcac tctttttgtt
     9421 agaccaatct aacaaaaaat acttgccaca tttgttaggt tattctaaat taatctccac
     9481 caagacaccg cacggtgttc tcagcaaaca gttccgctac cccggcgtta aggggaaatg
     9541 aggtcagcat ggatactatc gatcttggca acaacgaatc tctggtgtgc ggcgtgttcc
     9601 caaaccagga cggtacgttc accgcaatga cgtataccaa aagcaaaacg cttaaaaccg
     9661 aatctggcgc gcgtcgctgg ttagccagaa acactgactg atgaggttga cgatggaatt
     9721 taaagattta ccaataccat tccaggaaat ggcagcgaat atagttcgtt ctcaattggc
     9781 tactcctgac ctgagtaccg tagaaaaaga aaccatcgat aatatatccg gtaacgtgcg
     9841 ccgagccttt atcgggctat acgaagagaa gcagctctct gataaccagg atttacatga
     9901 aaaatacttt ctggaattaa tggacatcat taataaggga tttggcctgt taatgaaaaa
     9961 gaaagggatt cgaatagccc cccttgaaaa tcactttaca gcaagcagta ttaattcctg
    10021 tgatttaaag catcacacat ccgatgggaa agttgaatcc acctataaaa tatcaattaa
    10081 tcattaattt attcacgggt gaggtagagt gcgtgcgccg gacacgggt
//
LOCUS       DAZKHC020000132        10119 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZKHC020000132 DAZKHC020000000
VERSION     DAZKHC020000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.132"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(88..252)
                     /locus_tag="ACHOSM_RS20715"
                     /old_locus_tag="pgaptmp_004143"
     CDS             complement(88..252)
                     /locus_tag="ACHOSM_RS20715"
                     /old_locus_tag="pgaptmp_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HGU4560522.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFGSVNDNKY
                     LNAMWRSGKK"
     gene            complement(263..559)
                     /locus_tag="ACHOSM_RS20720"
                     /old_locus_tag="pgaptmp_004144"
     CDS             complement(263..559)
                     /locus_tag="ACHOSM_RS20720"
                     /old_locus_tag="pgaptmp_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HGU4560523.1"
                     /translation="MPAPLYGADDARRCSGNSVSEVLDKFRKNYDLIMSLPQETKEEK
                     EFRHCIWLAEKEECERIYQTSIRPFRKATYTHFPEYIDPRLRNYRSRYGAISND"
     gene            complement(583..966)
                     /locus_tag="ACHOSM_RS20725"
                     /old_locus_tag="pgaptmp_004145"
     CDS             complement(583..966)
                     /locus_tag="ACHOSM_RS20725"
                     /old_locus_tag="pgaptmp_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560524.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     TSGNSQQQQSSNHQNHPEYDDSIPF"
     gene            complement(966..1571)
                     /locus_tag="ACHOSM_RS20730"
                     /old_locus_tag="pgaptmp_004146"
     CDS             complement(966..1571)
                     /locus_tag="ACHOSM_RS20730"
                     /old_locus_tag="pgaptmp_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HGU4560525.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKLSPTPEQVLKAFTDAALQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
     gene            complement(1828..1980)
                     /gene="kil"
                     /locus_tag="ACHOSM_RS20735"
                     /old_locus_tag="pgaptmp_004147"
     CDS             complement(1828..1980)
                     /gene="kil"
                     /locus_tag="ACHOSM_RS20735"
                     /old_locus_tag="pgaptmp_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HGU4560526.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(1965..2096)
                     /locus_tag="ACHOSM_RS20740"
                     /old_locus_tag="pgaptmp_004148"
     CDS             complement(1965..2096)
                     /locus_tag="ACHOSM_RS20740"
                     /old_locus_tag="pgaptmp_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HGU4560527.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            complement(2121..3089)
                     /locus_tag="ACHOSM_RS20745"
                     /old_locus_tag="pgaptmp_004149"
     CDS             complement(2121..3089)
                     /locus_tag="ACHOSM_RS20745"
                     /old_locus_tag="pgaptmp_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HGU4560528.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVRDRLDELKARA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAERERIEAEQRAQ
                     REAKEAAERAEREKHAAIEEERRKAQEEAERIRREAEAKEQARIAEEKRIKGEEERRA
                     KDKAHRKEVNNKILADLIKVGASEDVAKNIITAIVKGEVFATKITY"
     gene            complement(3222..3383)
                     /locus_tag="ACHOSM_RS20750"
                     /old_locus_tag="pgaptmp_004150"
     CDS             complement(3222..3383)
                     /locus_tag="ACHOSM_RS20750"
                     /old_locus_tag="pgaptmp_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560529.1"
                     /translation="MMNKKYIVEVIERETKEVIKHFEFDNYRKADRVEEGLLRQSNLE
                     KFDVVMRCE"
     gene            complement(3380..3658)
                     /locus_tag="ACHOSM_RS20755"
                     /old_locus_tag="pgaptmp_004151"
     CDS             complement(3380..3658)
                     /locus_tag="ACHOSM_RS20755"
                     /old_locus_tag="pgaptmp_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560530.1"
                     /translation="MKEFKGTPGKWKYTVRNVNEMMTTFHGVTIGDTYIEAATRNERE
                     DALLIAAAPDLLEALQELVFLYEHAEGCRELTEYKRAKAAISKALGEE"
     gene            complement(3698..3949)
                     /locus_tag="ACHOSM_RS20760"
                     /old_locus_tag="pgaptmp_004152"
     CDS             complement(3698..3949)
                     /locus_tag="ACHOSM_RS20760"
                     /old_locus_tag="pgaptmp_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560531.1"
                     /translation="MEFHESAIYDFRANANSVKPQPIAVLFKTMGAWAVLCFAADDTD
                     ARMAIGQEMEMDPTNDEFIIYGAPSNYLLDTCNIYNKAA"
     gene            complement(4423..4529)
                     /locus_tag="ACHOSM_RS20765"
                     /old_locus_tag="pgaptmp_004153"
                     /pseudo
     CDS             complement(4423..4529)
                     /locus_tag="ACHOSM_RS20765"
                     /old_locus_tag="pgaptmp_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
     gene            complement(4944..5255)
                     /locus_tag="ACHOSM_RS20770"
                     /old_locus_tag="pgaptmp_004154"
     CDS             complement(4944..5255)
                     /locus_tag="ACHOSM_RS20770"
                     /old_locus_tag="pgaptmp_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560532.1"
                     /translation="MNGDRYEFTKDSTEDSVFHVTINGDKSSVYESVSGVYPEMKYTA
                     LSSNTMVGEYQSGGGITVETWSITTDKKALYSKVMNIPGMQQLTSTKSFVGDVVGTCN
                     Q"
     gene            complement(5345..5977)
                     /locus_tag="ACHOSM_RS20775"
                     /old_locus_tag="pgaptmp_004155"
     CDS             complement(5345..5977)
                     /locus_tag="ACHOSM_RS20775"
                     /old_locus_tag="pgaptmp_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU4560533.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFIGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGRKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            6081..6296
                     /locus_tag="ACHOSM_RS20780"
                     /old_locus_tag="pgaptmp_004156"
     CDS             6081..6296
                     /locus_tag="ACHOSM_RS20780"
                     /old_locus_tag="pgaptmp_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4560534.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            6416..6709
                     /locus_tag="ACHOSM_RS20785"
                     /old_locus_tag="pgaptmp_004157"
     CDS             6416..6709
                     /locus_tag="ACHOSM_RS20785"
                     /old_locus_tag="pgaptmp_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HGU4560535.1"
                     /translation="MQLTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMAMLLAVLEWGVVDDEMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            6742..6903
                     /locus_tag="ACHOSM_RS20790"
                     /old_locus_tag="pgaptmp_004158"
     CDS             6742..6903
                     /locus_tag="ACHOSM_RS20790"
                     /old_locus_tag="pgaptmp_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560536.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPKGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            6890..7780
                     /locus_tag="ACHOSM_RS20795"
                     /old_locus_tag="pgaptmp_004159"
     CDS             6890..7780
                     /locus_tag="ACHOSM_RS20795"
                     /old_locus_tag="pgaptmp_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HGU4560537.1"
                     /translation="MGVVKLADYRHNPVQHQEASSMGYVSIHRQFMDSRLYKDSQAVH
                     LWLHLILKANHESIVVNTDIGPITVDRGQMITGRPSLVRETFIPDNKVRSLLRTFESK
                     GMLNICSMGKKFSLFTIVKYDDFQAKNCPTVVQRLSNANTSNGAALSGDCPTVVQRLS
                     INNNINNISNTDVLESATADKKSDKKKPSVSCQDVVDAYHEILPEAPRIRALNDKRKN
                     QIRTFWRKAGVITRQLDGHGFTMQDWRNYLSYVGENCRWMFEERPNHQRGTVWHKKGF
                     DFLLNDNTYLKVREGEHDDR"
     gene            7770..9206
                     /locus_tag="ACHOSM_RS20800"
                     /old_locus_tag="pgaptmp_004160"
     CDS             7770..9206
                     /locus_tag="ACHOSM_RS20800"
                     /old_locus_tag="pgaptmp_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HGU4560538.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKILFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTATQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDLLYIDDTPGLSLAQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPGETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            9282..9488
                     /locus_tag="ACHOSM_RS20805"
                     /old_locus_tag="pgaptmp_004161"
     CDS             9282..9488
                     /locus_tag="ACHOSM_RS20805"
                     /old_locus_tag="pgaptmp_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560539.1"
                     /translation="MVKLIFSALRILGAMWMVATFIVVASSFVRLVGEGKDLVGVLFG
                     SILMWVIIGVMPVAVAKMAWRFVS"
     gene            9506..9832
                     /locus_tag="ACHOSM_RS20810"
                     /old_locus_tag="pgaptmp_004162"
     CDS             9506..9832
                     /locus_tag="ACHOSM_RS20810"
                     /old_locus_tag="pgaptmp_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560540.1"
                     /translation="MDESRKQFEEWFENYTGCDPKNKIYANMVEMYWQAWQASRAAIE
                     LDIDWPESNDDFWKDGEEGAYAMGHEDGRDKTVIAVMKAIRAAGIKEKNFDEANIHTS
                     QRSNQK"
     gene            9789..>10119
                     /locus_tag="ACHOSM_RS20815"
                     /old_locus_tag="pgaptmp_004163"
     CDS             9789..>10119
                     /locus_tag="ACHOSM_RS20815"
                     /old_locus_tag="pgaptmp_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HGU4560541.1"
                     /translation="MKQIYILRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKA"
BASE COUNT         2539 a         2377 c         2279 g         2924 t
ORIGIN      
        1 attcagcgtt tgtttcgtta acctttaaat cacttgggat gcatttacct ttcagaaatc
       61 catccatctc aattagtgac atttgtttca tttcttccca ctccgccaca tcgcattcag
      121 atatttgttg tcattaacag aaccgaaact atttctctta agcaattcct ctctcgatgg
      181 cattggcttt acgcgttggc gaataatcat ttctgccgga agaatgccgg gattgtatgc
      241 aagtcctctc atggtaaatt cctcagtcat tactgatagc gccatagcgt gagcggtaat
      301 tacgcaggcg cgggtcgata tattcaggga agtgggtata tgtggcttta cggaatggtc
      361 ggattgatgt ctggtaaatt cgctcgcatt cttctttctc tgcaagccat atacagtggc
      421 gaaactcctt ttcctctttc gtttcctgcg gtagcgacat tatcaggtcg tagttttttc
      481 tgaatttatc cagcacctcc gatacggaat tgccggaaca gcggcgcgcg tcatccgcac
      541 catacaaagg cgctggcata attaaatcct tatttttcta aatcagaatg ggatggaatc
      601 gtcgtattca ggatgatttt gatgattgct actttgctgc tgttggctgt ttcctgaagt
      661 tgcaaatcca atctttgcat tcagtaattc aagagtgatt gattgaccat tttgcccctg
      721 ataaacatca accctgatgt tttctccggt aatttctaca atgccacctt caacaagaac
      781 actacggtag taatccgctt gcgctcccgg cttggcaaat acaacggcgc tgtagtttgt
      841 ccattctttc ttttttgtct ggcgatcgta atactgaacg ccagcacgga tgttgaaacc
      901 gatattttcc ccggcctgaa actctcttgc gggtttgttt agtcttacag taatcgaatg
      961 tgccattaag cagccgctcc ttctaattcg tctcgtctga tgttgtaaac gtcctgcgct
     1021 ttgtgctgct ccggtgtgcc ttcgagcatc ttccacgctt tggcgaacgc ctgtttaagc
     1081 tcttctacgg tgtttttctg caatgctgcg tcagtgaatg cttttagaac ctgttcaggt
     1141 gtaggtgata gttttgattg ctttgctgct gcgttctgct gatgtttatg ctcgtcggta
     1201 tctgcatctt tcgcatcatc aatgccgaac aaaccattga ggcaatactt gcgtgcataa
     1261 gagcttgtag ctcccgtaac ttgtgcagaa tccattcctt tcttgctttc ttcctctcgt
     1321 gcaagggcgg ttgccgtatg actgttttcg ccatcggtaa tagttgccgt ggctttcacg
     1381 taataccgat caccaatcaa cacaacttca tcgctgattg ataaaaacag gccattcagt
     1441 aacggcttaa cgccttcaag aatgtcttcg cagcttctgt atttatattt accgaatgag
     1501 ttgtactgat tctttggcgc gttcagattc tcctgaatag ctgccagtct tgcgtaaaat
     1561 tctttgctca tatgattgtt ctcagaatgg acacggccca aggaaataac gctgatttaa
     1621 tacttcgact cgggacaagt taaggcatac ccgcattcct tcgcggtcgc cattatgtcg
     1681 ataccagaga gctttctgcg tgtacatgcg cctctgtaac ttgctctcct tcactgtggt
     1741 tgcaagtgac atgaatatct ccttcgttac cgattaattc tttcatctga cgaatgaatt
     1801 cttcgtctga ccagttatct gtaaaactca tttcctgcga taccatggaa ggttgatagc
     1861 tgatttcatc gctttatttg cttcaagcca catttttgaa tcaccaataa atctggctat
     1921 tactgctttg ttctgtgcag cacgaagcat ctggtgattg atggctattt cattgcgcat
     1981 aataagacct caactctttt ccatccgtca cgtaatttac gggtgattcg ttcaagtaaa
     2041 gattcattta gttggaaggc acccatgcga gcgcctcccg cgattgcgta aatcatgggt
     2101 ggttccttat attggtttta ttagtaggtt atttttgttg cgaatacttc gccttttacg
     2161 atggctgtta tgatattttt agcaacatct tctgatgcgc caaccttgat aaggtcagca
     2221 agtattttgt tatttacttc tttccggtga gctttatcct ttgctctacg ctcttcttcg
     2281 cccttgattc ttttttcttc tgctattctg gcttgctctt ttgcttcagc ctcgcgccgg
     2341 attcgttctg cctcctcctg tgcttttcgg cgttcttctt caattgccgc atgcttttct
     2401 ctttcagctc gttctgctgc ctcttttgct tcgcgctgtg ctcgttgctc ggcttcaatg
     2461 cgttcacgct ctgcacgttc cgctgcggcc ttagcttctg cttctcgcct tgctgctgct
     2521 tcaatttcgg cttttgcctt tgcttcggct tctgctctgg ctttctcttc agcttctctt
     2581 tttaagcgtt cttcatgctc tcgcttttcc tgctccgctt tgagtcttgc ctcttctctt
     2641 tggcggtcaa attcgcgatc catcaaaatc gctatttcat ggtcagactc aatttgcttt
     2701 gcgagagctt cagctgctgc cttagcttct tcttcggctt taatccgtgc ctgttcctcc
     2761 tcataatcag taagaggctg gcgcgccctg gctttcagtt catcaaggcg atcgcgcact
     2821 gtcttgcggt tggcatcaat tagctttgga atttccttca gttcagcaac aaggtctttg
     2881 ccaagaccat cgagatatgt tttcgtctgc gcaactttat acgccagaga agcgatctcc
     2941 tttctgccct ttgccgttgt gatatcaggc acaaaggaca taacttcacg ttcaaccttt
     3001 tgaaggattt cttcaatctg gtcggcagac tgaaatacag tcattgcatt tgctttttca
     3061 ataacaacta aatctgttac ttcactcata tatcctccat caaaaaaatc gccctcacac
     3121 tggagggcaa agaagatttc caataatcag aacaagtcgg ctcctgttta gttacgagcg
     3181 acattgctca catagcagac tcgcaaatct gctataggtg cttattcgca tcgcatgaca
     3241 acatcaaatt tttcgagatt actttgtcgc aacaatccct cttctacgcg gtcagctttt
     3301 ctataattat caaattcgaa atgtttaatt acttctttcg tttctcgctc tataacttca
     3361 acaatgtatt tcttattcat cattcttccc ccagggcctt gctgatggct gccttggctc
     3421 gtttgtattc ggttaactct ctgcaccctt cggcatgctc gtaaaggaag acaagctctt
     3481 gcagtgcttc aagcaaatca ggagctgctg ctatcagtag cgcatcctcc ctttcatttc
     3541 ttgttgctgc ttcaatgtat gtgtcaccaa tcgtcacacc atggaacgta gtcatcatct
     3601 cgttgacgtt tctaaccgtg tacttccatt taccaggcgt acccttaaac tctttcatat
     3661 ccacctctgt tgtttatgcc aaaaataaag gccaccgtca ggcagccttg ttgtaaatgt
     3721 tgcaggtatc aagtaagtaa ttagatggag cgccataaat tatgaattca tcgtttgtcg
     3781 ggtccatctc catctcttgg cctattgcca ttcttgcgtc agtgtcatca gcggcgaagc
     3841 ataaaacagc ccacgccccc attgttttaa aaagaactgc aattggctgt ggttttactg
     3901 aatttgcgtt agcgcgaaaa tcataaatcg cactttcatg aaactccata tatcacctta
     3961 aataagtggt ttgctgcgaa agtaaatacg cttaagttac ctgttattta tcccaccaag
     4021 ttccgtatct atctatccag ttacaccaat catcgacatt ccattttgct gtgtcgcatt
     4081 ttggcaattg gcatgaatat ctaccttctt tgtaaagtcg gcgtttgact ttcttgagca
     4141 tggctcacct caatcgtaat aagctggaat tgattttccg cgttgcttct ggcgaccaac
     4201 acaagtcaca cccatttcac cgcgtggctt gctgtaccat gtgcgctgat tcttgcgctc
     4261 aatacgttgc aggttgcttt caatctgttc gtggtattca gccagcaccg taaggtctat
     4321 cggattcagt gcgctttcta ctcgtgattt cggtttgcga ttcagcgaga gaatagggcg
     4381 gttaactggc tttgcgctta ccccaaccaa caggggattt gctgctttcc attgagcctg
     4441 tttctctgcg cgacgttcgc ggcggcgtgt ttgtgcatcc atctggattc tcctgtcagt
     4501 tagctttggt ggtgtggtgg ctggtagtct agctccagct tgttgagtct cattcggagg
     4561 ggtataaccg gcaccccagt gatttttcca tgcgacaacg tgcgcgttat ggcggcctta
     4621 tcgcccgcgg ctccccatct cgtccacgct attgctagcg ttgggagcgc ttcaccgctc
     4681 aacagtaggt aagcacttgc cagtgactag ctggcttcac cacaccccaa agccttctgc
     4741 tttgaatgct gcccttcttc agggctaaat ttttaagagc gtcaccttca tggtggttag
     4801 tgcgtcctgc tgatggctta aaattacaag aaagattgta tgttgtaaac aagaaaaatt
     4861 gtaaaaagag gcgtgaaaaa caaactccgt tgtttttaaa cggaaaatag tttgtttttt
     4921 ggttatcgag attgaggtgg ggattactgg ttgcaggttc cgactacatc accaacaaag
     4981 gatttggttg atgtaagttg ttgcatacct gggatattca ttactttgga gtaaagagct
     5041 tttttgtctg tagtgattga ccaagtttca acggttattc ctcctccaga ctggtattct
     5101 cctaccatag tgttcgatga caaagcagtg tatttcatct ctggatagac gccagaaact
     5161 gattcataaa ctgatgattt atcgccattt attgttacgt ggaaaacgga atcttccgtg
     5221 ctgtcttttg taaactcgta acgatcgcca ttcattgccc cgtacccgtg caggtttgtg
     5281 acaatccagc attcagaatt ggcgctggta gttaagagca ttgagagtag cgccgcaatc
     5341 ctgatcatac gaattttacc ctcgcttcca cgacaacacc gataatcttg cagttcccgt
     5401 tgataggagt cataggccat gaaggattca ggcctttcag gtacttccgc ccgccatcta
     5461 taaccagttt cttgaatgtt gcttcgttcg cgtcagtcag tttggctaca acaaggcttc
     5521 cattcactgg ctcgcgtcca gtatctacta acaccatatg accttcaggg atgctttgac
     5581 ctacaggtga ggtcatggaa tcaccttcaa ccttcagcca gaatccatcc cctaataagt
     5641 taacgtcact gtcataccat tcatcaatgt ccttgatatc gtagggttca caagcttcac
     5701 accacgaacc agctctaacc atgctaatca atggatattt ccctttgggc tcaacgtgcc
     5761 caataaatct aacattcgaa tcagaggtgc cattgagcag ccagtcaaca cttacgccaa
     5821 gagctgacgc aagttctggt aaaaagcgtg gtcgcttagt tttaccgttt tcgagctgct
     5881 ctatagactg ctgggtagtc cccacctttt gagcaagttc agcctggtta agtccaagct
     5941 gaattctttt gctttttacc ctggaagaaa tactcataag ccacctctgt tatttacctc
     6001 caatcttcac aagaaaaact gtatttgaca aacaagatac attgtatgaa aatacaagaa
     6061 agtttgttga tggaggcgat atgcaaactc tttctgaacg cctcaagaag aggcgaattg
     6121 cgttaaaaat gacgcaaacc gaactggcaa ccaaagccgg tgttaaacag caatcaattc
     6181 aactgattga agctggagta accaagcgac cgcgcttctt gtttgagatt gctatggcgc
     6241 ttaactgtga tccggtttgg ttacagtacg gaactaaacg cggaaaagcc gcttaagaca
     6301 ttcccgctct tacacatccc agccctgaaa aagggcatca aattaaacca cacctatggt
     6361 gtatgcattt atttgcatac attcaatcaa ttgttatcta aggaaatact tacatatgca
     6421 acttacaagt actcgcaaga aagcgaatgc aattacaagc aacatcctga atcgaattgc
     6481 tgtacgtggt cagcgaaagg ttgccgacgc gttagggatt aatgaatcgc aaatttcgcg
     6541 atggaaagac agctttatcc caaagatggc catgcttctg gctgttcttg aatggggagt
     6601 tgttgatgat gagatggctc gattggcacg acaagttgct tcgattctca ccaataaaaa
     6661 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gaattctgag gtcattactg
     6721 gatcaatcca caggagtcat tatgacaaaa cgtcgtaaga aataccagga aaaagaagag
     6781 attcgacacc ctgattcacc taagggatta gtggtagccg cagcaaataa cagggcgttc
     6841 gcagagcgcc ttgttggtgt ttacagacta gccaaagcag gagtgaaaca tgggcgtcgt
     6901 taagttagct gattacaggc ataaccctgt acaacatcag gaggcatcca gtatggggta
     6961 tgtctctata caccgccagt ttatggacag caggctctat aaggactctc aggcagtaca
     7021 tctttggctt cacttaatcc tcaaggctaa tcacgaatct attgtcgtca atacggatat
     7081 cggtccgata actgttgatc gcggtcagat gataactgga cgcccgtcgc tggtcagaga
     7141 aacattcatc cccgacaaca aagttaggag cttattacgg acttttgagt cgaaagggat
     7201 gcttaatatt tgctcgatgg ggaagaaatt tagcctgttt acaatcgtta aatatgacga
     7261 ttttcaggca aaaaattgtc caacggttgt ccaacggttg tccaacgcaa acaccagtaa
     7321 tggcgcggct ctcagcggag attgtccaac ggttgtccaa cggttgtcca taaacaataa
     7381 tataaataat atctctaata ctgacgtatt agagagtgcc acagcagaca aaaagtctga
     7441 caagaaaaaa ccttccgtta gctgtcagga tgttgtcgat gcttaccacg aaatccttcc
     7501 tgaagcgcca agaatccgcg cactgaatga caagcgtaaa aaccagatcc gaacgttctg
     7561 gcgcaaagcc ggagtgataa cccgccagct tgacgggcat gggttcacga tgcaggactg
     7621 gagaaattat ttgagctacg taggcgaaaa ttgccgatgg atgttcgaag aacgcccaaa
     7681 ccatcaacgc ggaaccgtct ggcacaaaaa gggatttgat ttcctgctta acgacaatac
     7741 ctacctgaaa gttcgtgagg gtgaacatga tgaccgataa tttttatgcg ccgccccata
     7801 gcatcgaggc agagcaggcg gtgattggtg gattgcttct ggatgatgac agcagtgagc
     7861 gcgtccagaa ggttctggcg atgctgaagc ctgactcatt ttacagccga ccacacaaaa
     7921 tccttttcga agaaataacc agaatgcacc gggagcaaaa gccagtagat ggcctgacgc
     7981 ttttcgatga actggagcgt aaatcgttaa cggcgtctgt tggcggtttt gcttatatcg
     8041 ctgagatcgc aaagaacacg ccaagcgccg caaacatcgt tgcctatgca atgcaggttc
     8101 gtgaaaccgc aatggaacgc tacgccatca accgcatgac tgaagcgacg gaattgctct
     8161 attcccgcaa cggaatgact gcaacgcaga agtacgaagc tattcaggcg attttcacgc
     8221 aactgacaga ccatgcaaaa accggatcgc gtcgcggcct tcgctcattt ggtgaggtca
     8281 tggaagactg ggttagcgac cttgagaagc gatttgaccc gtcaggtgaa caacgaggaa
     8341 tgagcacagg aataccatcg ctggacagga tgctgtcacc gaaaggtctg gtgaaaggct
     8401 ctctgttcgt cattggcgct cgccctaaga tggggaaaac gacgctatac agccagatgg
     8461 caatcaactg cgcagtgcat gagaaaaagc ccgctctgat gttcagcctt gaaatgccag
     8521 gtgatcagat actggaaaaa ctggtagggc agaagtctgg tgttaacccg aatatttttt
     8581 accttccggc gacaaatgac gctgatgacg gctatcaggg tgattacgat ggtgacttca
     8641 acagggcgat cgaaacagcc aatcgcttga gtgaaatcga cctgctttac atcgacgaca
     8701 cgccgggatt atctctggct caaatcgtca gcgaaagccg tcgaatcaag cgagaaaaag
     8761 gatgtgttgg catgattctg gtcgattacc tgacactaat gaccgctgag aaggccgatc
     8821 gcaacgacct tgcttacggc atgatcacca aaggactgaa gaaccttgcc aaagagcttg
     8881 attgcgttgt tgtgcttctg acgcagctta accgcgcact ggaaagccga accaataaac
     8941 gcccattacc aagtgactca cgagatacag ggcagattga acaggattgc gattattggg
     9001 ttgggatcca tcgtgaaggc gcttttgatg acagtgttcc acctggtgaa accgaactaa
     9061 tccttcgtct caatcgtcat ggcaataccg gcacggtgta ttgcattcag gcaaatggcg
     9121 ctatttatga cacagaccaa cagtctgctg aaatgcgccg acgtgaacgc gaggaaccgc
     9181 agtccaagaa gaaaggagga ttctgatgac catctacatc actgagttaa taacaggcct
     9241 gctggtaatc gcaggccttt ttattttggg gagagtaaat cgtggttaag ttaattttct
     9301 ctgcattgag gattctcggt gctatgtgga tggtggcgac gttcattgtg gttgccagta
     9361 gttttgtccg gttggtaggc gaaggtaaag acctggtggg tgtgcttttc ggtagcattc
     9421 tcatgtgggt gattatcggt gttatgcctg tcgctgtagc aaaaatggcg tggcgttttg
     9481 tgagttgaac tgagggtaag tatcgatgga cgaatcaaga aagcagtttg aagaatggtt
     9541 tgaaaattac accggatgtg atcctaaaaa taaaatatac gccaatatgg ttgagatgta
     9601 ttggcaagcg tggcaggcat cgcgagcagc tattgaactg gatatcgact ggcccgaatc
     9661 gaatgacgac ttttggaaag atggtgaaga aggtgcttat gcgatggggc atgaggatgg
     9721 gagggataaa acggtaattg cagtgatgaa agctatcaga gccgctggaa ttaaagagaa
     9781 gaatttcgat gaagcaaata tacatacttc gcaacgaagc aatcagaaat aacgccatag
     9841 acgccattct ctcactaccc atcgacgaca agtcacccca cgaagtccac gttaaagaac
     9901 ccaagcgcag caaagcgcag aatgaccgta tgtggccgat gctgaacgat gtttcgcgtc
     9961 aggtgctatg gcatggtcaa cggctggcgc cggaagactg gaaagacctg ttcactgccc
    10021 tgtggcttaa gaccaaaaaa ctggagcaac gaagtgtgcc tggcatcgac ggtggcgttg
    10081 tcatgcttgg cgtgcgtacc agcaaaatgc ggaaggcca
//
LOCUS       DAZKHC020000133        10078 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZKHC020000133 DAZKHC020000000
VERSION     DAZKHC020000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.133"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            427..1404
                     /gene="glaH"
                     /locus_tag="ACHOSM_RS20820"
                     /old_locus_tag="pgaptmp_004164"
                     /pseudo
     CDS             427..1404
                     /gene="glaH"
                     /locus_tag="ACHOSM_RS20820"
                     /old_locus_tag="pgaptmp_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
     gene            1424..2692
                     /gene="lhgO"
                     /locus_tag="ACHOSM_RS20825"
                     /old_locus_tag="pgaptmp_004165"
     CDS             1424..2692
                     /gene="lhgO"
                     /locus_tag="ACHOSM_RS20825"
                     /old_locus_tag="pgaptmp_004165"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HGU4560542.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2715..4163
                     /gene="gabD"
                     /locus_tag="ACHOSM_RS20830"
                     /old_locus_tag="pgaptmp_004166"
     CDS             2715..4163
                     /gene="gabD"
                     /locus_tag="ACHOSM_RS20830"
                     /old_locus_tag="pgaptmp_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4560543.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNLLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4177..5457
                     /gene="gabT"
                     /locus_tag="ACHOSM_RS20835"
                     /old_locus_tag="pgaptmp_004167"
     CDS             4177..5457
                     /gene="gabT"
                     /locus_tag="ACHOSM_RS20835"
                     /old_locus_tag="pgaptmp_004167"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HGU4560544.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGFSEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLVIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5768..7168
                     /gene="gabP"
                     /locus_tag="ACHOSM_RS20840"
                     /old_locus_tag="pgaptmp_004168"
     CDS             5768..7168
                     /gene="gabP"
                     /locus_tag="ACHOSM_RS20840"
                     /old_locus_tag="pgaptmp_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HGU4560545.1"
                     /translation="MGQSSQPHELGDGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLIYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7189..7851
                     /gene="csiR"
                     /locus_tag="ACHOSM_RS20845"
                     /old_locus_tag="pgaptmp_004169"
     CDS             7189..7851
                     /gene="csiR"
                     /locus_tag="ACHOSM_RS20845"
                     /old_locus_tag="pgaptmp_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HGU4560546.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGDRVASMSELELLDIFDARANMEAMLVSLAIARGGYEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSYYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7852..8301)
                     /gene="kbp"
                     /locus_tag="ACHOSM_RS20850"
                     /old_locus_tag="pgaptmp_004170"
     CDS             complement(7852..8301)
                     /gene="kbp"
                     /locus_tag="ACHOSM_RS20850"
                     /old_locus_tag="pgaptmp_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HGU4560547.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKRVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTTTPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8385..8543)
                     /locus_tag="ACHOSM_RS20855"
                     /old_locus_tag="pgaptmp_004171"
     CDS             complement(8385..8543)
                     /locus_tag="ACHOSM_RS20855"
                     /old_locus_tag="pgaptmp_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HGU4560548.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8726..9025
                     /locus_tag="ACHOSM_RS20860"
                     /old_locus_tag="pgaptmp_004172"
     CDS             8726..9025
                     /locus_tag="ACHOSM_RS20860"
                     /old_locus_tag="pgaptmp_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGU4560549.1"
                     /translation="MTELAQLQASAEQAAALLKVMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            9035..9559
                     /gene="ygaP"
                     /locus_tag="ACHOSM_RS20865"
                     /old_locus_tag="pgaptmp_004173"
     CDS             9035..9559
                     /gene="ygaP"
                     /locus_tag="ACHOSM_RS20865"
                     /old_locus_tag="pgaptmp_004173"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HGU4560550.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9606..10010)
                     /gene="stpA"
                     /locus_tag="ACHOSM_RS20870"
                     /old_locus_tag="pgaptmp_004174"
     CDS             complement(9606..10010)
                     /gene="stpA"
                     /locus_tag="ACHOSM_RS20870"
                     /old_locus_tag="pgaptmp_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HGU4560551.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2333 a         2581 c         2731 g         2433 t
ORIGIN      
        1 tagaggcgga tctctaaata tcaatattcc tgggcgtagc gctcttcttt tagggaaaac
       61 aggagaaccg ccgaactatc tctatttata atttaatgtt atatcagccc cgataaaacg
      121 gggcagataa tatgtttagt ttactaacgg tcattttgca gtgaagccat ttactgtttt
      181 ttatcgacca gataatctgt tctctgatgt taactccccc taacctgttg ctttagttat
      241 tcatttcctg tctcactttg ccttaatacc ctacgttaaa tgttactaat ttgttgcttt
      301 tgatcacaat aagaaaacaa tatgtcgctt ttgtgcgcat ttttcagaaa tgtagatatt
      361 tttagattat ggctacgaaa tgagcatcgc catgtcaccc tacatctcat aagaggatcg
      421 cttctgatga atgcactgac cgccgtacac aataacgctg tcgattcagg ccaggactat
      481 agcggattca ctctcatccc gtcggcgcaa tccccgcgtc tgctggaact caccttcacc
      541 gaacagacga ccaaacagtt tctcgaacag gttgccgaat ggcccgtgca ggcgctggag
      601 tacaaatcgt ttctgcgttt tcgggtaggc aaaattcttg acgatctgtg tgcgaatcag
      661 ctgcaaccat tgctgttgaa gaccctgcta aaccgtgctg aaggtgcgct gttgatcaat
      721 gcggtgggta tcgatgatgt ggcgcaggca gatgagatgg tgaagctggc aacggcggta
      781 gcgcatctga ttggtcgctc aaatttcgat gcgatgagcg gtcagtatta cgcgcgtttc
      841 gtggtgaaaa atgtcgataa ctcagacagc tatctgcgtc agccgcaccg cgtgatggaa
      901 ctgcacaacg acggcacgta tgtcgaagag atcaccgatt acgtgctgat gatgaaaatc
      961 gatgagcaaa acatgcaggg tggaaattcg ttgctgctgc atctcgatga ctgagaacat
     1021 ctggaccact atttccgcca tccactggcg cgtcgcccaa tgcgctttgc cgcgccgccg
     1081 agcaaaaacg tcagcaaaga tgttttccat ccagtgttcg acgttgatca acagggtcgc
     1141 ccggtgatgc gctatatcga ccagttcgtc cagccgaaag acttcgaaga aggcgtgtgg
     1201 ttgagcgagc tttcggacgc cattgaaacc agcaaaggca ttctttctgt acccgttccc
     1261 gttggcaaat tcctgttgat aaacaacctg ttctggctgc acggtcgcga ccgctttact
     1321 ccgcacccgg atctgcgccg tgaactgatg cgtcagcgcg gctacttcgc ttacgccact
     1381 caccactacc agacgcatca gtaagcgaaa aggaattgag cggatgtatg attttgtgat
     1441 tattggcggc ggcatcatcg gcatgtcgac cgccatgcaa ctgattgatg tctatccgga
     1501 tgcccgtatt gcgttgctgg aaaaagagtc cggcccggcc tgtcaccaga cgggccacaa
     1561 cagcggcgtg atccatgccg gggtctatta cacgcccggc agcctgaagg cgcagttttg
     1621 cctggcggga aaccgcgcca ccaaagcctt ttgcgatcaa aacgacattc gctatgacaa
     1681 ctgcggcaag atgctggtcg ccacgtccga tctcgaaatg gaacggatgc gcgcgttgtg
     1741 ggaacgcacg gcggcgaacg gtatcgagcg cgagtggtta aacgccgagg aactgcgcga
     1801 gcgcgaaccg aatatcaccg ggctcggcgg catttttgtg ccgtccagcg gcattgtcag
     1861 ctatcgcgaa gtcacagcgg caatggcaaa aatcttccag gccagaggcg gtgagattat
     1921 ttataacgcc gaagtcagcg ccctcagtga gcataaaaac ggcgtggtcg tccgaacccg
     1981 tcagggcggc gaatatgaag catcgacgct gattagctgt tccgggctga tggctgaccg
     2041 gctggtgaaa atgctcggcc tcgaaccggg ttttattatc tgcccgttcc gtggcgagta
     2101 tttccgcctt gcgccggagc ataaccagat tgttaaccat ctgatttacc ccatccccga
     2161 cccggcaatg ccgtttttgg gcgtgcatct cacccgcatg atcgacggca gcgtgaccgt
     2221 cgggccaaac gcggtgctgg cttttaaacg cgaaggctat cgcaagcgcg acttctcatt
     2281 tagcgacacg ctggaaattt taggctcgtc ggggattcgc cgggtgctgc aaaaccatct
     2341 acgctcagga ctgggcgaga tgaaaaactc gctgtgcaaa agcggctatc tgcggctggt
     2401 gcaaaagtat tgtccccggc tttcgttaag cgatctccag ccctggcccg ccggtgtgcg
     2461 ggcgcaggcg gtatcgccgg acggcaagct gattgacgat tttctgtttg tcaccacccc
     2521 gcgcacgatc cacacctgca atgcgccctc cccggcagcg acatcagcaa ttcctattgg
     2581 tgcgcatatt gtcagcaagg tacaaacgct gttggcaagc cagagtaacc ccggacgcac
     2641 gctgcgagcg gcacgtagtg tggatgcctt acacgccgca tttaatcaat aacctttgaa
     2701 aacaggacgt aacaatgaaa cttaacgaca gtaacttatt ccgccagcag gcgttgatta
     2761 acggggaatg gctggacgcc aacaatggcg aggtcatcga cgtcaccaat ccggcgaacg
     2821 gcgacaagct gggtagcgta cccaaaatgg gcgctgatga aacccgcgcc gctatcgacg
     2881 ccgccaaccg cgctctgccc gcctggcgtg cgctcaccgc caaagaacgc gccaacattc
     2941 tgcgcaactg gttcaatttg atgatggagc atcaggacga tttagcgcgc ctgatgaccc
     3001 tcgaacaggg caaaccactg gccgaagcga aaggcgaaat cagctacgct gcctctttta
     3061 ttgagtggtt tgccgaagaa ggcaaacgta tttatggcga taccattccc ggtcatcagg
     3121 ccgataagcg cctgattgtt atcaagcagc cgattggcgt taccgcagct atcacgccgt
     3181 ggaacttccc ggcggcgatg attacccgta aagccggtcc ggcgctggcg gcaggctgca
     3241 ccatggtgct gaagcccgcc agtcagacac cgttctctgc gctggcgctg gcggagctgg
     3301 cgattcgcgc gggcattccg gctggcgtgt ttaacgtggt caccggttcg gcaggcgcgg
     3361 tcggtaatga actgaccagc aacctgctgg tacgcaaact gtcgtttacc ggttcgaccg
     3421 aaattggccg ccagttaatg gaacagtgcg cgaaagacat caaaaaagtg tcgctggagc
     3481 tgggcggcaa cgcgccgttt atcgtctttg acgatgccga tctcgataaa gccgtggaag
     3541 gcgcgctggc ctcgaaattc cgcaacgccg ggcaaacctg cgtctgcgcc aaccgtttat
     3601 acgtgcagga cggcgtgtat gaccgctttg ccgaaaaatt gcagcaggcg gtgagcaaac
     3661 tgcacatcgg cgacgggctg gataaaggcg tcaccatcgg gccgctgatc gatgaaaaag
     3721 cggtagcaaa agtggaagag catattgccg atgcgctgga gaaaggtgcg cgtgtggttt
     3781 gcggcggtaa agcggatgaa cgcggcggca acttcttcca gccgaccatt ctggtggacg
     3841 tgccggccaa cgccaaagtg tcgaaagaag agacgttcgg ccccctcgcc ccgctgttcc
     3901 gctttaaaga tgaagccgat gtgattgcgc aagccaatga caccgagttt ggccttgccg
     3961 cctatttcta cgcccgtgat ttaagccgcg tcttccgcgt gggcgaagcg ctggagtacg
     4021 gcatcgtcgg catcaatacc ggcattattt ccaatgaagt ggccccgttc ggcggcatca
     4081 aagcctcggg tctgggtcgt gaaggttcga agtatggcat cgaagattac ttagaaatca
     4141 aatatatgtg catcggtctt taactggaga atgcgaatga acagcaataa agagttaatg
     4201 cagcgccgca gtcaggcgat tccccgtggc gttgggcaaa ttcacccgat tttcgctgac
     4261 cgcgcggaaa actgccgggt gtgggacgtt gaaggccgtg agtatcttga tttcgcgggc
     4321 gggattgcgg tgctcaatac cgggcacctg catccgaagg tggtggctgc ggtggaagcg
     4381 cagttgaaaa aactgtcgca cacctgcttc caggtgctgg cttacgagcc gtatctggag
     4441 ctgtgcgaaa taatgaatca gaaggtaccg ggcgatttcg ccaaaaaaac gctgctggtc
     4501 accacaggtt ctgaagcggt ggaaaacgcg gtgaaaatcg cccgcgccgc caccaaacgt
     4561 agcggcacca tcgcctttag cggcgcgtat cacgggcgca cgcactacac gctggcgctg
     4621 accggtaagg tgaatccgta ctctgcgggc atgggcctga tgccgggcca tgtttatcgc
     4681 gcactttatc cttgcccact gcacggcttc agtgaagatg acgctatcgc cagcattcac
     4741 cgaattttta aaaatgacgc tgcgccggaa gatatcgccg ccatcgtgat tgagccggtt
     4801 cagggcgaag gcggtttcta cgccgcgacg ccagccttta tgcagcgttt acgcgcgctg
     4861 tgtgacgagc acgggatcat gctgattgcc gatgaagtgc agagcggcgc ggggcgtacc
     4921 ggcacgctgt ttgcgatgga gcaaatgggc gtggcaccgg atctcaccac ctttgcgaaa
     4981 tcgatcgcag gcggtttccc tctggcgggc gtcaccgggc gcgcggaagt gatggacgct
     5041 gttgcaccgg gcggtctggg cggcacctat gccggtaacc cgattgcctg cgtggcagcg
     5101 ctggaagtgt tgaaggtgtt cgagcaggaa aatctgctgc aaaaagccaa cgatctgggg
     5161 cagaagttga aagacggatt gctggtgata gccgaaaaac acccggagat cggcgacgta
     5221 cgcgggctgg gagcgatgat tgccatcgag ctgtttgaag atggcgatca cagcaaaccg
     5281 gacgcaaaac tcaccgccga gatcgtggca cgcgcccgcg ataaaggcct gattcttctc
     5341 tcctgcggcc cgtattacaa cgtgctgcgc atccttgtac cgctcaccat tgaagacgct
     5401 cagatccgtc agggtctgga gatcatcagc cagtgttttg ctgaggcgaa gcagtagcta
     5461 tgtccgtttg taagagttaa acgttgctaa gggaatgata acgatgcagt aaaaaataat
     5521 aacgatgagg cgatcgacaa agttaaaaac gactgcggac tgtcccctcg cccctctggg
     5581 aagagggtta gggtgagggg taatcacgcg gctacaccct caccccggtc ctctccctaa
     5641 gggagaggga gaaaaccctc ccgctttcgt ttttaagatt ttgttgaccg tctcaattaa
     5701 acgcgtgacc cgacgcggga aatgtcgggg cgctctccca agtgacacac tttcgagagg
     5761 attcaggatg gggcaatcat cgcaaccaca tgagttaggc gacgggctga agtcacgcca
     5821 cgtcaccatg ttgtctattg ccggtgttat cggcgcaagt ctgtttgtcg gttccagcgt
     5881 cgccatcgcc gaagcgggcc cggcggtatt actggcctat ctgttcgccg gattactggt
     5941 ggttatgatt atgcggatgt tggcggaaat ggcagttgcc acgcccgata ccggttcgtt
     6001 ttccacctat gccgataaag ccattggccg ctgggcgggt tataccatcg gctggctgta
     6061 ctggtggttt tgggtactgg ttatcccgct ggaagccaac atcgccgcta tgatcctgca
     6121 ctcgtgggtt ccaggcattc ccatctggtt attttccctc gtcattaccc tcgccttaac
     6181 tggcagtaat ttattaagcg ttaaaaacta cggcgaattt gagttctggc tggcgctgtg
     6241 caaagtcatc gctatcctgg cctttatttt ccttggtgca gtcgcaatta gcggttttta
     6301 cccttatgcc gaagtgagcg ggatctcaag attgtgggat agcggcggct ttatgcccaa
     6361 cggtttcggt gcggtattaa gcgcgatgtt gatcaccatg ttctcgttta tgggcgcaga
     6421 aattgtcacc attgccgccg cggaatccga cacgccggaa aaacatattg tccgcgccac
     6481 taactcggtt atctggcgta tttctatctt ctatttgtgc tctatttttg tcgtcgtggc
     6541 attaattccg tggaatatgc ccggactaaa agccgtcggt tcttatcgct cggtactgga
     6601 attgctcaat attccccatg cgaaattaat catggactgt gtgatattac tttccgtaac
     6661 cagctgtctg aactcggcgc tgtataccgc gtcaaggatg ctctactcct taagccgtcg
     6721 cggtgatgcg cccgcggtaa tgggcaaaat caaccgcagt aaaaccccgt atgtggcggt
     6781 gttactctcc accggagcgg catttttaac ggtggtggtg aactattacg cacctgcgaa
     6841 agtgtttaaa ttcctgatag acagctccgg tgctatcgcc ctgctgattt atttagtcat
     6901 cgccgtttca cagttgcgga tgcgtaaaat tctgcgagca gaaggaagcg aaattcgctt
     6961 gcgcatgtgg ctttacccgt ggctcacctg gctggtaata ggctttatta cctttgtgtt
     7021 ggtggtgatg ttattccgcc cggcgcaaca gttagaagtg atctctaccg gcttattagc
     7081 gatagggatt atctgtaccg tgccgattat ggcgcgctgg aaaaagctgg tattgtggca
     7141 aaaaacaccc gttcataata cgcgctgata atgatcagga gtcacaccat gaccattacg
     7201 tctctggatg gctatcgctg gctgaagaac gatattattc gcggtaattt tcaaccggat
     7261 gaaaaattac gaatgagttt gctgacatcg cgttatgcac ttggcgttgg gccgttacgg
     7321 gaagctcttt cgcaactggt ggcggaacgg ctggtcacgg tggtgaatca aaaaggggat
     7381 cgggtggcgt ctatgtcaga gctggagctg ctcgatattt tcgacgcccg cgccaatatg
     7441 gaagcgatgc tggtcagtct ggcgattgcc cgcggtggct atgaatggga ggcagacgtt
     7501 ctcgcaaaag cgcatctgct cagtaagctt gaggcctgcg acgccagtga gaaaatgctt
     7561 gatgagtggg atctgcgtca tcaggcgttt catactgcaa ttgtggcggg ctgtggttct
     7621 tactatttgc tgcaaatgcg tgaacggttg tttgatctgg cggcgcgcta tcgatttatc
     7681 tggctgcggc gaacagtgct ttcggtggaa atgctggagg ataaacacga tcagcaccag
     7741 accctgactg cggcggtact ggcgcgagat accgcgcgcg ccagtgagtt aatgcgccag
     7801 catttactga cgccaattcc cattatccag caggcgatgg ctggcaatta actactcttc
     7861 aggaatacgc aacacttgcc ccgggtaaat tttatccggg ctttttagca tcggtttatt
     7921 cgcttcgaag attttattgt acagattggc gttaccgtag acctgtttgg aaatggcact
     7981 cagagtgtcg ccagacttaa cggtataaaa ctggctggca gtggctggtg tcgtcgtttt
     8041 cacctgatca tcgacactgg caataccgga aatattcccc accgcaacaa ggattttctc
     8101 cttcgcctcc tgactcaggc cgtcacccgt gaccgtcgct ttgccgtcag caatttgaat
     8161 attcacttta tcggcatcag gtataccggt tttgttcaga tgctcctgca ccctcttcgc
     8221 ctgatcgtct ttatcgtgct gacctgtaac cgcgtcccag agtttttctc cggcatcttt
     8281 cacaaaattg aacagaccca tagctacctc attcgttaac ggaaacactc agaaagtgta
     8341 gcagaggaac gcggtgcgtt aacgccgcgg gggtgaacac aggtttaatc gcgggtttgc
     8401 acccaaaacg cgtgaatcaa accggggata tacccaagca gtgtcagcag aatattaata
     8461 atgaacgccc aaccgaaccc tttaccgagc agcacgccga gcggcggcag aatgatggtg
     8521 atgacgattc tccagaaacc catatgtact ccctataaga aaattactca ttgtttaaaa
     8581 agagatttta tctcttaagc gtagtaattt tagcggagcc tgccagtttc agcctacgct
     8641 tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt agagtttgct
     8701 taatttagaa aacacttaaa taactatgac tgaactcgcg caattacagg ccagtgccga
     8761 acaggcagcg gccttattga aagtaatgag ccaccctaaa cggttgctga ttctgtgcat
     8821 gcttagcggt tcccccggca ccagcgcggg agagctgact cgcattaccg gactgagtgc
     8881 ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatcg acagccaacg
     8941 ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca ttatcgccac
     9001 cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca tttcgccgca
     9061 tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg acgctgatga
     9121 atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc tggaacagtc
     9181 aggtcttccg gctaaattac gtcgcgagca aattatattc cactgccagg caggtaaacg
     9241 caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa tatttttact
     9301 cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa ataaatctca
     9361 acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat taatcggcgt
     9421 tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg ttggtgccgg
     9481 gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag ataagatgcc
     9541 gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac atccggcctc
     9601 agtaattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg agcaattggc
     9661 ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc ggtgaattta
     9721 tatttcgccg gacgcggctg gcgtttttta ccagcgcgtg gtgcagcagc agagctatta
     9781 cccaataact cttccgggtt aattccgtca gccttcatca gctccagcca ggtgctaatt
     9841 ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg tctttcttta
     9901 gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa ttcgcgagcc
     9961 atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca ttacggacat aataaagaaa
    10021 accttttaac gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat cacttatt
//
LOCUS       DAZKHC020000134        10027 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZKHC020000134 DAZKHC020000000
VERSION     DAZKHC020000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.134"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(63..803)
                     /gene="cysQ"
                     /locus_tag="ACHOSM_RS20875"
                     /old_locus_tag="pgaptmp_004175"
     CDS             complement(63..803)
                     /gene="cysQ"
                     /locus_tag="ACHOSM_RS20875"
                     /old_locus_tag="pgaptmp_004175"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HGU4560552.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            993..2936
                     /gene="cpdB"
                     /locus_tag="ACHOSM_RS20880"
                     /old_locus_tag="pgaptmp_004176"
     CDS             993..2936
                     /gene="cpdB"
                     /locus_tag="ACHOSM_RS20880"
                     /old_locus_tag="pgaptmp_004176"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HGU4560553.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGMVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3065..3445)
                     /gene="ytfH"
                     /locus_tag="ACHOSM_RS20885"
                     /old_locus_tag="pgaptmp_004177"
     CDS             complement(3065..3445)
                     /gene="ytfH"
                     /locus_tag="ACHOSM_RS20885"
                     /old_locus_tag="pgaptmp_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGU4560554.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3534..4394
                     /gene="qorB"
                     /locus_tag="ACHOSM_RS20890"
                     /old_locus_tag="pgaptmp_004178"
     CDS             3534..4394
                     /gene="qorB"
                     /locus_tag="ACHOSM_RS20890"
                     /old_locus_tag="pgaptmp_004178"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU4560555.1"
                     /translation="MIAITGATGQLSHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            4502..5467
                     /gene="ytfF"
                     /locus_tag="ACHOSM_RS20895"
                     /old_locus_tag="pgaptmp_004179"
     CDS             4502..5467
                     /gene="ytfF"
                     /locus_tag="ACHOSM_RS20895"
                     /old_locus_tag="pgaptmp_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4560556.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            5575..6237
                     /gene="ytfE"
                     /locus_tag="ACHOSM_RS20900"
                     /old_locus_tag="pgaptmp_004180"
     CDS             5575..6237
                     /gene="ytfE"
                     /locus_tag="ACHOSM_RS20900"
                     /old_locus_tag="pgaptmp_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HGU4560557.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6282..7694)
                     /gene="cycA"
                     /locus_tag="ACHOSM_RS20905"
                     /old_locus_tag="pgaptmp_004181"
                     /gene_synonym="dagA"
     CDS             complement(6282..7694)
                     /gene="cycA"
                     /locus_tag="ACHOSM_RS20905"
                     /old_locus_tag="pgaptmp_004181"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HGU4560558.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(8002..8622)
                     /gene="fklB"
                     /locus_tag="ACHOSM_RS20910"
                     /old_locus_tag="pgaptmp_004182"
     CDS             complement(8002..8622)
                     /gene="fklB"
                     /locus_tag="ACHOSM_RS20910"
                     /old_locus_tag="pgaptmp_004182"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4560559.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8840..9478
                     /gene="ytfB"
                     /locus_tag="ACHOSM_RS20915"
                     /old_locus_tag="pgaptmp_004183"
     CDS             8840..9478
                     /gene="ytfB"
                     /locus_tag="ACHOSM_RS20915"
                     /old_locus_tag="pgaptmp_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HGU4560560.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(9462..9863)
                     /locus_tag="ACHOSM_RS20920"
                     /old_locus_tag="pgaptmp_004184"
                     /pseudo
     CDS             complement(9462..9863)
                     /locus_tag="ACHOSM_RS20920"
                     /old_locus_tag="pgaptmp_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT         2578 a         2608 c         2522 g         2319 t
ORIGIN      
        1 ttttaggttg ggagaaatca ggatggcaga atcaggaaat acactgtttc tgccatctga
       61 atttagtaaa tagacactct gaaccccgga ttcaggaacg actcgcgcgg agtgtaatcc
      121 agcggtttac cctgccagtc gtgaacgtgc gctccggcag ctgcagccac agcatgtcca
      181 gcggcggtgt cccaaatatt cgttggtccg aagcgcgggt acagctgcgc ctgtccttcc
      241 gccaccaggc agaatttcag cgaagagccg atggacgtgg tctgatgttc gccaagctgt
      301 tgcagatact ctttcagctc cgcatctgca tgggaacggc tgataaccac cagcggcggg
      361 cgcgcatcgc ggacctgaat ctgcttgcgc acaccgcact cttctttcca ggccttgcct
      421 tctgccgcgc tgtacattac gttcattacc ggcgcataca ccacacctaa aatcggtttg
      481 ccatggtcaa tgagcgcaat gttaacggtg aattcgccat tacgtttaat aaactcttta
      541 gtaccatcca gcgggtctac cagccagtaa cgctgccagt gctgacggac ttcccaaccg
      601 ggaggatctt cttcagaaag gaccggaata tccggtgtca gcgtacgtaa accgtccatg
      661 ataacggtgt gtgcggcaat atccgctgcc gttaccggag aattgtccgc tttgctgacg
      721 acgtccatcg gtttcgtccc gtcgtagacc tgcataatgg catcgcctgc attccgtgca
      781 agctggcata cttgatctaa catttctcca cctcgtctct gtgagcggtg ttaacttatt
      841 gttttactta taccctatcg ttaatgaatg cgccaactgt gatagtgtca tcattttcaa
      901 agcgtaaaat tgtggcattc ttcactgttc tataagtaag gcgtttattc tcccttttct
      961 ttcgtattcc cgatgataaa aggatgtccc tgatgattaa gtttagcgca acgctcctgg
     1021 ccacgctgat tgccgccagt gtgaatgcag cgacggtcga tctgcgtatc atggaaacca
     1081 ctgatctgca tagcaacatg atggatttcg attattacaa agacaccgcc acggaaaaat
     1141 tcggactggt acgtacggca agcctgatta acgatgcccg caatgaagtg aaaaacagcg
     1201 tactggtcga taacggcgat ttgattcagg ggagtccgct ggccgattac atgtcggcga
     1261 aaggattaaa agcaggtgat gttcatccgg tttataaggc gctgaatacg ctggattata
     1321 cggtcggtac actcggcaat catgaattta actacggtct ggattacctg aaaaatgcgc
     1381 tggcgggagc gaaattccct tatgtaaatg ccaacgtcat tgacgccaga accaaacagc
     1441 caatgtttac accgtattta attaaagata ccgaagtggt cgataaagac ggaaaaaaac
     1501 agacgctgaa gattggctat attggcgtcg tgccgccgca aatcatgggc tgggataaag
     1561 ctaatttatc cggaaaagtg acggtgaatg atattaccga aaccgtgcgc aaatacgtgc
     1621 ctgaaatgcg cgagaaaggt gccgatgtcg ttgtcgttct ggcgcattcc gggctgtctg
     1681 ccgatccgta taaagtaatg gcggaaaact cagtttatta cctcagtgaa attccgggcg
     1741 ttaacgccat tatgtttggc catgctcacg ccgttttccc gggtaaagat tttgctgata
     1801 tcgaaggggc tgatatcacc aaaggcacgc tgaatggtgt tccggcggta atgccgggca
     1861 tgtggggcga tcatcttggg atggtcgact tacaactcag taatgacagc ggtaaatggc
     1921 aggtgacgca ggcgaaagcg gaagcacggc cgatttacga catcgccaat aaaaaatccc
     1981 tcgcggcgga agacagcaag ctggtagaaa cactcaaagc cgatcacgat gccacacgcc
     2041 agttcgtcag caagccaatc ggtaaatctg ccgacaatat gtatagctat ctggcgctgg
     2101 tgcaggacga tccgaccgtg caagtcgtga acaacgcgca aaaagcgtat gtcgaacatt
     2161 acattcaggg cgatccggat ctggcaaaac tgccggtgct ttcagctgcc gcaccgttta
     2221 aagttggtgg tcgcaaaaat gacccggcaa gctatgtgga ggtggaaaaa ggccagttga
     2281 ccttccgtaa tgccgccgat ctttatctct accccaatac gctgattgta gtgaaagcca
     2341 gcggtaaaga ggtgaaagag tggctggagt gctccgccgg acagtttaac cagattgatc
     2401 ctaacagcac gaaaccacag tcactcatca actgggatgg tttccgcact tataactttg
     2461 atgttattga tggtgtgaat tatcagattg atgttaccca acccgcccgc tatgacggcg
     2521 agtgccagat gattaatgcc aatgcagagc ggattaagaa cctgaccttt aatggcaagc
     2581 cgattgatcc gaacgctatg ttcctcgttg ccaccaataa ctatcgcgct tacggcggca
     2641 aatttgccgg tacgggcgac agccatatcg cttttgcttc accggatgag aaccgctcgg
     2701 tgctggcagc gtggattgct gatgagtcga aacgtgcggg agaaattcac ccggcggcag
     2761 ataacaactg gcgtttagca ccgatagctg gcgataagaa actggatatc cgtttcgaaa
     2821 cttccccgtc agataaagcc gcagcgttta ttaaagagaa agggcaatat ccgatgaata
     2881 aagtcgcgac cgatgatatc gggtttgcga tttatcaggt ggatttgagt aagtaaaaca
     2941 cttctttttc ggtctataaa ttatcaaccg catccggcat ttattggcgg atgcgatgct
     3001 ggcgcatctt atccgcccta caagccatgc accgtagacc agataagctc agcgcatccg
     3061 gcagttatgc cgcacgttca tcccgcaccg ccaacacctc gggcaaattc aactcaatcc
     3121 agtccgccag tgcagcaacc ttttcgctca cctgctcgcc cagcggcgtg aggctatatt
     3181 ccacatgcgg cggcaccacc ggatacgcga tacggttaag aaaaccatcc tgttctaacg
     3241 cctgtaacga ctgcgcaagc atcttttcac tcaccccacc gattttgcgc cgcaggtcgc
     3301 taaagcgatg agtaccttcg cgaagcgcca ccagaatcaa caccccccaa cggctggtga
     3361 cgtgtttcaa cacctcgcgc gacgggcact gttccgcaaa gagattcccc tctttcagtt
     3421 gttgcgacag gctaacctga ctcatttcat acttaccttt ttgtacgtac ttactaaaag
     3481 taagtttagg tgttagcgta tttaaacaca agacaaaacg atggagactt cccatgatcg
     3541 ctattactgg tgccactggc caacttagtc actatattat tgaatccttg atgaaaacgg
     3601 ttcctgccag ccaaatagtg gctatcgttc gtaatccggc aaaagcccag gccctggcag
     3661 cacaaggcat taccgtgcgt caggctgact acggcgatga agccgcactg acatctgcac
     3721 ttcagggagt ggaaaaacta ctgctgatct cttccagcga agtgggtcaa cgtgccccgc
     3781 agcatcgtaa tgttattaat gccgcaaagg cggctggcgt gaaatttatc gcttatacca
     3841 gcctgctaca tgcagatacc tccccgctcg gcctcgccga tgagcacatc gagacggaga
     3901 aaatgctggc tgattctggc atcgtttaca ccctgctgcg caacggctgg tacaccgaaa
     3961 actacctcgc cagcgccccg gcagcactgg aacacggcgt atttatcggt gcggcgggcg
     4021 atggcaaaat cgcctcagca acgcgggcag attatgcggc agctgcggca cgcgtgatta
     4081 gcgaagccgg tcacgaaggc aaggtttacg aactggcggg cgatagtgcc tggacgttga
     4141 cacagttagc ggcagagctg accaaacaga gcggaaaatc cgttacctat caaaatctga
     4201 gcgaagccga tttcgccgcg gcactgaaaa gcgtcggact gcccgacgga ctggcggata
     4261 tgctggcgga ttctgacgtt ggcgcatcga aaggtggtct gtttgataac agcaaaacgc
     4321 ttagcaaatt gattggccgc ccaacgacaa cgttagccga aagcgtaagc catcttttta
     4381 atgttaataa ctagttaatt aaagtggcat cctcccgcat cctctctaat aatgacggga
     4441 tgccgggagc aatcatgtct gcttcctgaa ctttcttctg acagaccaat ggatgccagt
     4501 aatgattagc ggcgtgctgt acgccctgtt agcagggttg atgtgggggc ttatttttgt
     4561 cgggccgttg atcgtaccgg aatacccggc gatgttgcag tcgatggggc gttatctggc
     4621 gttagggtta attgcgctgc ccattgcctg gctgggacgc gtgcgtctgc gtcagttggc
     4681 gcgtcgcgac tggcttaccg ccttgatgct cactatgatg ggcaacctca tctattactt
     4741 ctgccttgcc agtgccattc aacgtactgg tgcgcctgtt tccacgatga ttatcggcac
     4801 cctgccggtg gtgattcccg tttttgccaa tctgctttat agccagcgcg acggcaaact
     4861 cgcgtgggga aaactcgccc cggcactggt ttgtattggc atcggcctgg cgtgtgtgaa
     4921 tattgctgag ttaaaccacg gactccccga ttttgactgg gcacgttata cctcaggcat
     4981 cgtgctagcg ttagtttccg tggtctgctg ggcatggtat gccctgcgca acgcccgctg
     5041 gctgcgggag aatcccgaca aacatccgat gatgtgggcg acggcgcagg cgctggtcac
     5101 actgccggtt tctctcatcg gctatctcgt cgcttgttac tggctgaata cgcaaacgcc
     5161 ggacttctcc ctaccttttg gcccccgtcc gctggtgttt attagtctga tggttgcgat
     5221 agccgtgctt tgctcatggg ttggcgcact ctgctggaac gtcgccagcc agcgattacc
     5281 gacagtgatt ctcgggccgc tgatcgtttt cgaaacactg gcaggtttgc tgtacacctt
     5341 tttgatacgc cagcaaatgc cgccgctgat gacgctgagc ggtatcgcgc tgttagtggt
     5401 tggcgtggtc attgcagtca gagcaaaacc ggaaaagcct ttaactgaat ctgtctcaga
     5461 aagttgacat actggctgtg agttaaataa gcctctgcta cgtatgggtt atagcttttg
     5521 ccttaaagat gcatttaaaa tgcatcttat cttattaaga atgaggtatc agctatggct
     5581 tatcgcgacc aacctttagg tgaactggcg ctctctattc ctcgcgcttc agctctgttt
     5641 cgtaaatatg atatggatta ctgctgtggc ggtaagcaaa cgctggcgcg cgcggcggca
     5701 cgtaaagaac tggatgttga ggtcattgaa gctgaactgg caaagcttgc tgaacaaccg
     5761 attgagaaag actggcgtag cgccccgctg gcagaaatca ttgaccatat catcgtgcgc
     5821 taccacgatc gtcaccgcga gcaactgccg gagctgatcc tgcaagcgac taaagtcgag
     5881 cgcgttcacg ccgacaaacc gagcgtgcca aaagggctga caaaatacct gaccatgctg
     5941 catgaagagc tttccagcca catgatgaaa gaagagcaga tcctcttccc gatgatcaaa
     6001 caaggcatgg gcagccaggc aatggggcca atcagcgtaa tggaaagcga gcacgatgaa
     6061 gcgggcgaac tgctggaagt gattaaacac accaccaata acgtcacacc gccgccagaa
     6121 gcctgcacca cctggaaagc gatgtataac ggtattaatg aactgattga tgacctgatg
     6181 gatcacatca gtctggaaaa caatgtactg ttcccacgcg ccctagcggg tgagtgatct
     6241 aaaagctgga tggcattgcg ccatccagca tgataatacg gttatttccg cagttcagca
     6301 gcccgcttct taccaataaa caaccagccc aaccccagcg cgataaacca cagcggggta
     6361 accagcagcg cctggcgagt gtcatcttcc agtgtcagca acacgaccac gaacacaaag
     6421 aacgccatac atacccagca catcagcttg ccgagcggca tcttgtagat cgacttttca
     6481 tgcagatgag gacgctgttt gcggtacacc aggtacgagc aaaggataat cgtccagacg
     6541 aacataaaca gaatcgcgga aacggttgta atcatcgtga acgcgccaat cacgctagga
     6601 ttcacataca acatcaccac gccgccaagc agacagatac acgagaaggt caaccctttc
     6661 gcgggtactg cgcgcttaga aagtttagcg aacgctttcg gcgcaacacc ttcctgcgcc
     6721 agaccaaaca gcatacggct ggtagagaag acaccgctgt tagcggaaga cgccgcagag
     6781 gtcagcacca caaagttgat cacgctggca gccgcaggca aaccaaccaa cacaaacagc
     6841 tcaacgaacg ggcttttctc cgggactacc gaactccacg gtgtcacgga cataatcaca
     6901 atcagcgcga agacgtagaa cataatgata cggatcggaa tggagttaat cgcgcgtggc
     6961 agtgatttct ccggatcttt ggtttccgca gctgttgtac ctaccagctc aatccccacg
     7021 aaagcgaaaa ccgctatctg gaatccggca aagaagccac ttaaaccttt cgggaaccag
     7081 ccgccgtcat tccacaaatg cgcgaatgac gcttccacac cggtcggtga ctgaaagtgc
     7141 atcgccacca tgaccaggcc gacgacaatc agcgacacga tggcgacgat tttgatcatc
     7201 gcaaaccaga actccatctc accgaacatt ttcacggtgg caaggttgag cgtcagcagc
     7261 agcactatca ccgccagcga ggcgacccag tcggagagat cggggaacca gaactgagca
     7321 taagccgtga tcgccaccac gtctgccata ccggttacaa cccagcagaa ccagtaagtc
     7381 cagccggtga aatatcccgc ccacggcccc agtaaatcgg aagcgaagtc actaaaagat
     7441 ttgtattcca gattcgaaag cagcaattcc cccattgccc gcatcacgaa aaagagcata
     7501 aaaccaatga tcatataaac gaaaatgatc gacggcccgg caaggctaat cgttttgccg
     7561 gaccccataa acaacccagt accaatggca ccgccaatgg caataagctg aatatgtcgg
     7621 tttgtgagat tgcgccgtag cgactgttca gccggagcct gatcatcggc aacgactttt
     7681 acctgatcta ccatgttttt tcttcctgta cctgtctgtg ttgttcaggc tctacggcct
     7741 ttagtcagtc tatgatacga cgaatctgta tcgggactca tcgatattag gtaacaatac
     7801 gcgggatgaa tactaagatt tagatctaat gttaatttta tgtttaaagt gagtgtcata
     7861 tcacggtgat aacgcgaaac agctcacaaa aatacacgct tacggggtct ttgcaacaca
     7921 aaatagcgcc gtttggcaaa ctgaagggtt tattgctgaa tgcctgctcc cctctggttc
     7981 gaggggaaca gaatgcgctg cttagaggat ttccagcagt tcgacttcaa acaccagggt
     8041 gctgaacgga gggatggatg cacctgcgcc gcgctcgcca tatgccagtt cctgcgggat
     8101 ggtcagttcc catttagaac ctaccggcat cagagtcagt gcttcaatcc agccagggat
     8161 cacgccgtta accgggaatt cagcgggctc gccacgagca acggagctgt caaacacggt
     8221 gccgtcgatc agttttccgg tgtaatgaac acgaacgcgg tcggttcgtg ccggaattgc
     8281 gccttcaccc tggttgatca cgcggaattg caggccagat tcagtgctat tcacaccttc
     8341 ttttttggcg ttttcttcca ggtatttcac accttcagca gccatcgcct ggaaacgctg
     8401 acgacgaacg gcatcggcgc gttcgtggat ttcacgcagc gcgcgatgca ccacatcaac
     8461 cggaacagcc ggatgtttgc cttccagcgc atcggcaata cctgcaacca gtgcttctgg
     8521 cagcagccct tccaggccag attcactcag ttgttgccct acctgcaaac caatgccgta
     8581 gcttgcttgc gcttcgatgg tgtcaaaagt tggggtggtc atcgtatttc ctttcttagg
     8641 gtataaagta gcgggcagca tatcagccac acttctttgg gtaaaacttt gtcgcgggaa
     8701 tgatgacaaa tcacggggaa aaagaaacaa tagcaatatc ccgtatgaat ataataaagg
     8761 cgggtttacc acggacatca tagcgttagc ttattcatag gctatgattg aggaacaaga
     8821 cgcggagcag gaggaaacca tgcccgggcg ctttgaacta aaaccaaccc tggagaaagt
     8881 ctggcacgcg ccggacaatt ttcgcttcat ggacccgctg ccgccgatgc atcgtcgggg
     8941 gatcattatt gccgccattg tattggtggt cggatttctg cttccatctg atgatacgcc
     9001 caacgcgccg gtggtgacgc gtgaagcgca gttagatatt cagtcgcaat cacaaccgcc
     9061 gacagaagaa cagctgcggg cgcaattagt caccccacaa aatgatcctg atcaggtggc
     9121 tccggtcgct ccagaaccta tacaggaagg ccagccagaa gagcagccgc aaactaccca
     9181 aacgcaacca ttccagccag atagcggaat tgataatcaa tggcgctctt accgtgtcga
     9241 acccggtaaa acgatggcgc agctgttccg cgaccacgga ctgcccgcta ccgatgtcta
     9301 tgcaatggcg caggtagaag gcgcgggtaa gccgctgagt aatttgcaaa acgggcaaat
     9361 ggtgaaaatc cgccagaacg ccagcggcgt ggtgacgggc ttaaccattg atacggggaa
     9421 taatcaacag gtcctgttta cccgccagcc ggatggcagt tttattcgtg cgcggtagat
     9481 gaagggtcaa ccaaaagcca gctaaggatt gtctctttga tgaccttacg tctggcttcc
     9541 agtgcctggg gggaaaccat actgatatga taaaccaggc caagcgtatg ctggttgatc
     9601 aaataatatc ccccaagcgc agcaatatta atattcacct gcaatgggtc gatatctggc
     9661 ttaaaaatat tgtgtttctt cccttcatcc aataaggatt ccattaactg caagtgcgca
     9721 tggttaattt ctaagagacg cagtgatttt gcataatgca cacctttact ttgattttca
     9781 ctatgcacaa tttttaaaaa ccaagggtta gcaatataat aatcccatgt aaaatcaata
     9841 attttctcaa tggctttttg tggctggtta accagatgat taacaaacgc accgatacca
     9901 acagcgtaga ataattacat accgcacaac aagaaagtat ggtggaaggc gtggaagtca
     9961 ttttctccct gggggcatcg ttatatccat aatgagcgat ttttgatatt aatatctttc
    10021 accatcc
//
LOCUS       DAZKHC020000135         9785 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZKHC020000135 DAZKHC020000000
VERSION     DAZKHC020000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.135"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1372
                     /locus_tag="ACHOSM_RS20925"
                     /old_locus_tag="pgaptmp_004185"
     CDS             <1..1372
                     /locus_tag="ACHOSM_RS20925"
                     /old_locus_tag="pgaptmp_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anti-phage deoxyguanosine triphosphatase"
                     /protein_id="HGU4560561.1"
                     /translation="PKGTRMTHCKENTWYERQGYNTTRPNEIRSEYERDLSRLIHSSA
                     FRRLQSKTQVLGLGESDFYRTRLTHSMEVAQIGGGILSQLSKHDTDEGGKFLPDPSLM
                     QAICLAHDIGHPPFGHGGEVALNYCMRSYGGFEGNGQTLRILSKLDKYTETNGLDPTR
                     RLILGVLKYPASYSEVVNERAYSQLQPRSNEWLFKSSEFKPPKCYLDSEENTVRFALQ
                     PFDSDDVKLFKTVKTTALNKHKKTIYKALDTTIMDLADEISYSLHDLEDAISLKMIDK
                     KMWEEHFEDKSHLFAACQCDSFNLKADDVAEKLFGESYLRKECIGTLVHLMITSVKLE
                     VQNSDFKSKILRYKVALPAAVEELRKAIFELVKNKVIQHENVQLLEFKGQKLIVELFN
                     VLANDPIRFLPTETRKKYNQEEAKGDDKKMRVICDYVSGMTDDYATRLYEKIFVPRKG
                     SIFDRL"
     gene            complement(1580..1948)
                     /locus_tag="ACHOSM_RS20930"
                     /old_locus_tag="pgaptmp_004186"
     CDS             complement(1580..1948)
                     /locus_tag="ACHOSM_RS20930"
                     /old_locus_tag="pgaptmp_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013096817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560562.1"
                     /translation="MDALSEVFVNNWLPGICTFFLGIFYSNIVEKKKLKQKLKNDILE
                     IFIPVFNAGNEISIEIAENAYRNMNGTFQLYKRIYPGMFNKEAERELDRLLKDGFLIN
                     GEVNKHYFEPTNIESLIKRL"
     gene            complement(2155..2463)
                     /locus_tag="ACHOSM_RS20935"
                     /old_locus_tag="pgaptmp_004187"
     CDS             complement(2155..2463)
                     /locus_tag="ACHOSM_RS20935"
                     /old_locus_tag="pgaptmp_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560563.1"
                     /translation="MQIKIAAPLGGDAIIEFDDNEEVSGRLSIISGDITEDMIAEAIA
                     GANPNSYMGFVNTLDAPASDVLRTLHLYAGWFVDWPAVDGGDEDDDDDLGDHVDQFVY
                     "
     gene            complement(2453..5440)
                     /locus_tag="ACHOSM_RS20940"
                     /old_locus_tag="pgaptmp_004188"
     CDS             complement(2453..5440)
                     /locus_tag="ACHOSM_RS20940"
                     /old_locus_tag="pgaptmp_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560564.1"
                     /translation="MSLSDQVVMATSIETLIELLKNLPDYGRVSYVVTAKGDEVKTAF
                     DIVDASVLLVSNTLDGKINPDYPQELQPRDRTRASSLLQVNQISKDLRPAQLTDSGLS
                     SHGAPIIGEDNAVESGNGRTMGIIKAYQDGNADRYREYLIDHATEFGIRPEKVESMTA
                     PVLVRRRLTKVDRVQFAKDSNISDLQEMAASEKAFVDADSITPAMMALFNPSESGDLL
                     SRSNDAFIRGFMTQVGATQAAGLVTEDGRPTRQLVDRIQNAIFAKAYKDARLVRMVAE
                     EPDPDMRNVLTALNAAANDFVQMQALSGEAHKQAVTTIVDGIETADSLDKKALAALKD
                     AVDLVRQSKESGQHITDVIAQGDMFSETAPEVKALALFIVANNRSAKRMATAFKLMAQ
                     RINDELQHQGQALGDMFGGGDVSLQDILRQVSQELENEGMQGISGGLFESASGGSYNG
                     VAPYTSLLLHRASGIKDIIHLIRLLSRADPQDEQLVQVLAHFVRMPVADVKKWCRLFG
                     ISNSLLRGLLNHASSLGRDGFDEIAQAIKNGDMPPAIDWFSIRPTRVKAFLSAAHSAS
                     PLAEMVQRLSLIFTDHTALGDLTLDEMKEASIQWADQQNEVNSDFLPAFRKAVSKADD
                     ARGILKAFKALQSRVNKHVGDIDGVTAEGRDILKEHGITPEFIDEIRTDMQREVVSSL
                     QIVARALADANPKSAAIVNRVIGDIEASEGMGALKLFLSRAFNPNGNILPGIIGEAKK
                     YVSEEELEQLDQLLKRFSYNPQTRWQMNQRSMGSVHEKVLSAMNSAIANSSVSEEKAL
                     EWADSFITEEVEEARAGQNGGIDLRKELADIYRLTGGKISTLSKVVHHQGRAYANLNG
                     VVAVNLNDETASALWHELGHHLEYSNPGLLEKARSFLKANVEGDKPSFVNIGGRGKPE
                     WCFRSRLSNIYMAKVYPPASVSNTGKIRQKSPTISKTSATEVFSMALQLYHDKEAAAA
                     SLMNGDGLLELLLGVAKELNNAD"
     gene            complement(5453..5818)
                     /locus_tag="ACHOSM_RS20945"
                     /old_locus_tag="pgaptmp_004189"
     CDS             complement(5453..5818)
                     /locus_tag="ACHOSM_RS20945"
                     /old_locus_tag="pgaptmp_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ddrA"
                     /protein_id="HGU4560565.1"
                     /translation="MTLSAIELMDLSDKLDALMSKAATASGMELLDISDEIDQIMQQM
                     GYGASGGSSGEEKQPSVHDGVPKLVADFLADKFVDQSTDAFIGTLQDLSQYVGTYIDL
                     DQVKQHTAAWIAANIKEAA"
     gene            complement(5815..7734)
                     /locus_tag="ACHOSM_RS20950"
                     /old_locus_tag="pgaptmp_004190"
     CDS             complement(5815..7734)
                     /locus_tag="ACHOSM_RS20950"
                     /old_locus_tag="pgaptmp_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560566.1"
                     /translation="MEQFNINKGVTIKPGLDVLPPPVTDDEYRALMAGEDRYLMTESN
                     TLEEIEATFFYDTPIHWCATDLLEAISSTRLQLHRTMQAFVRALNQKLNGTGISAGSD
                     KTGNVAQSGARAIGGAEIGRARNVNGLPVLPAIIPLSDGQTISILFHSPTAENRITNS
                     DTLVAFQFLLNKKDVTHTVAPMSGRDMTLAQVTMKLANLAEKNSAKFQRAQKKKKALV
                     DEITQLQADSDQKEDAMSDLADQVAAVEGQKVDLEQKINAVASEADSLYEENERLQTE
                     IDQLNRTGGRETIAPAGMTGGHSRAMTDRLASIKNRMHMNGEVTLSNGASMKQFIEDG
                     EGYIQLTDSDGSVYMIKAKSIQGVDMADAIGKLFKAYKAGNVSEYLVQPEEHKPENVE
                     PEPAEDTSSSSPEPEVSVGAYRYALQMRPAAPGAIPEGNKAILPRPDEGDPYYEYARY
                     GIATYDTPLSDQQMSEYDLKLLPREDSFDFLAKTLTNGPFGKYAQKALELATSSPDEF
                     RVMLKTQFQKTFPNIAFPGGAGTEKMVQSMINALQAEVGEITQPEPAPAQPDETVSEA
                     DAEANKAIEYLNNVMDMQSTDMAEIRNARGNVREAIAALQAAGRFEENEELVNGAARH
                     LADLLVAIQKAGVAA"
     gene            complement(7736..8338)
                     /locus_tag="ACHOSM_RS20955"
                     /old_locus_tag="pgaptmp_004191"
     CDS             complement(7736..8338)
                     /locus_tag="ACHOSM_RS20955"
                     /old_locus_tag="pgaptmp_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="odaE"
                     /protein_id="HGU4560567.1"
                     /translation="MTKNKYATVDFDQVNEKGLKSLIAAINKTGVTVIEVDSSNRATT
                     KDGVKVKTAKLVLNDGQILAIQVNDTGDISSVKLNGKAIPNAQSPDIKTLGTVMGQAA
                     RKNSAKFQKSLIAKAKRVANPVDKKPAVKSNFQRLQEAKQRNAQVVAAYKSAQNSVSF
                     NQQQITDLRAKLDKETGRLNNEKARNGELKRRLKQLKAGN"
     gene            complement(8325..8768)
                     /locus_tag="ACHOSM_RS20960"
                     /old_locus_tag="pgaptmp_004192"
     CDS             complement(8325..8768)
                     /locus_tag="ACHOSM_RS20960"
                     /old_locus_tag="pgaptmp_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4560568.1"
                     /translation="MIGWGVCVLALALADRYLLKRKDITHLELGDVEIKPGFIRVPFK
                     YRSKFPFLRGATVRYWIRDVQKPTTVIEGEQRCLTSAEQGENSEWLYIPTEYMGKGER
                     LWHFNVMVTHGDSFINPLYRIFPVTQQIRRSYVINLAQDVSDDEK"
     gene            complement(8765..9094)
                     /locus_tag="ACHOSM_RS20965"
                     /old_locus_tag="pgaptmp_004193"
     CDS             complement(8765..9094)
                     /locus_tag="ACHOSM_RS20965"
                     /old_locus_tag="pgaptmp_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU4560569.1"
                     /translation="MLDTQELAPVAIALLLSVIGGIGTFLMDVRDGRQSGNLLGLVTE
                     IFVAVTAGAVAYLLGQHEGWELSITYLMVTIASNNGHEVISGMKRVNIDSILNVLTSL
                     VKKGGGK"
     gene            9024..9359
                     /locus_tag="ACHOSM_RS20970"
                     /old_locus_tag="pgaptmp_004194"
                     /pseudo
     CDS             9024..9359
                     /locus_tag="ACHOSM_RS20970"
                     /old_locus_tag="pgaptmp_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         2538 a         2469 c         2098 g         2680 t
ORIGIN      
        1 gcccaaagga acacgtatga cacactgcaa agaaaatact tggtatgagc gtcaagggta
       61 caatacaacc cgcccgaatg agataaggtc ggagtatgaa cgtgatcttt cccggttaat
      121 ccactcttcc gcttttagga gattacaatc gaagacgcag gtgttgggat tgggcgaaag
      181 cgatttctac cgtacgcggt taactcactc tatggaagta gcgcaaattg gcggcggtat
      241 cttgtctcag ttgtctaagc acgatacaga tgaggggggt aaattcttac ctgacccaag
      301 cctgatgcag gctatctgcc ttgctcatga tataggacat ccccccttcg gtcatggtgg
      361 tgaggtcgca ctaaactact gcatgagatc ctatggcggc tttgaaggta acgggcagac
      421 actgcgtata ctctccaaac tggataagta caccgaaacc aatgggctgg atcccacccg
      481 tagacttatt ttaggcgtac tgaaatatcc agcaagctat tcagaggtcg tcaatgagag
      541 ggcttacagc cagttacagc caagaagcaa tgagtggttg tttaaatcat ccgaatttaa
      601 gcccccgaaa tgttacctgg actctgagga gaacactgtt agattcgcac tccaaccttt
      661 tgatagtgat gatgttaagt tattcaaaac ggtaaaaact accgcgttga ataagcacaa
      721 gaaaacaata tataaagcac tggatacaac gattatggac ttggctgatg agatatccta
      781 ctcactccat gatcttgaag acgcaatttc tctgaagatg atcgacaaaa aaatgtggga
      841 ggagcacttc gaggacaagt cccatttatt tgcagcttgc caatgcgact cgtttaatct
      901 aaaagccgat gatgtagctg aaaaactgtt cggcgaaagt tatctacgca aggagtgcat
      961 agggacactt gttcatctga tgatcactag cgttaagctt gaagtgcaga actctgattt
     1021 caaaagtaaa attttacgct ataaagtggc acttcctgcc gcggtagaag agttacgtaa
     1081 agccattttt gagctggtta agaacaaagt tattcagcac gaaaacgtcc aactattaga
     1141 gttcaaaggt cagaaactga ttgtggaact attcaatgta ctggcaaatg acccaatacg
     1201 ttttctcccg acagaaaccc gaaagaaata taatcaagag gaagcaaaag gtgatgataa
     1261 aaaaatgcgc gtcatttgcg actatgtttc aggcatgaca gatgattacg ctactagact
     1321 gtatgagaaa atatttgtac cgagaaaagg ctctattttt gatagactct gatctttttc
     1381 tattatatag tacgtagccg ggttccagaa ccgcataaaa ttagatctgg atataattca
     1441 tttaattatc tgttatatgc caaacataac gatatataat agtggattag atgttacaac
     1501 ttgacgcaga ataaatagct tttattggcg catgatgcaa accccgcaat gaagcggggt
     1561 tttaactgtg gtaaatgagt tataatcttt taatcaggct ctcaatatta gttggttcaa
     1621 agtaatgctt attaacctct ccatttatga ggaaaccgtc ttttagtagt cggtctagct
     1681 cacgctctgc ttctttgttg aacatacctg gatatattct cttgtacaac tgaaatgtac
     1741 cattcatatt cctgtaagca ttctcagcaa tttcaatgga gatttcattt ccggcgttaa
     1801 atacaggaat gaatatctca agaatatcat tctttaactt ctgtttaagt ttcttttttt
     1861 caacaatatt agaataaaag atacctaaaa aaaatgtgca gataccagga agccaattat
     1921 taacgaaaac ctcagacaaa gcgtccacta tcttactcca gagttaagat tgattctcat
     1981 aacgcacaaa ctccgttcaa ggcgattttt atattacaaa ctatacataa acgctgatca
     2041 atatctccag attttgtgtg cctgtgcatg aatatgcact aatcataatc gaccagaact
     2101 tccgttaatc gctaaactcg aacgattcta gccacataga cgatctgtta gtatctaata
     2161 tacaaactga tccacatgat cacccaagtc atcgtcgtcg tcctcatcgc caccatctac
     2221 tgctggccaa tcaacaaacc agccagcgta aagatgcagc gttcggagaa catcacttgc
     2281 gggagcatca agggtgttaa cgaatcccat atagctattg ggatttgccc cagctatggc
     2341 ttcagcgatc atgtcctcgg taatgtcacc ggagataatg cttaaacgcc cggaaacttc
     2401 ttcgttatca tcaaattcga taatggcatc tccacctaat ggcgctgcga ttttaatctg
     2461 cattatttag ctcctttgcc acacctaata acagttccag caatccgtca ccattcatca
     2521 gtgatgcggc agcggcctct ttgtcatgat acaactgaag agccatagag aatacttccg
     2581 ttgctgacgt tttggaaata gtcggtgatt tctgccgaat tttcccggtg ttacttactg
     2641 aggctggtgg gtataccttc gccatataaa tattactcaa tcgagatctg aagcaccatt
     2701 caggcttgcc acgcccaccg atattaacga aagatggttt atccccttca acattggcct
     2761 tcaggaatga ccgggctttc tctaacaaac cagggttact gtactcaaga tgatgaccca
     2821 gctcgtgcca cagtgcactt gcagtttcat cgttcaaatt gacagcaaca acaccattta
     2881 gatttgcata tgcccttccc tggtggtgaa ctacctttga taaggtcgat attttcccgc
     2941 cggtcaggcg ataaatatca gcaagttcct tgcgcaggtc tatcccacca ttctgtccag
     3001 cgcgggcttc ttccacttct tctgtgataa aagagtcggc ccactcaaga gctttttctt
     3061 cagatacgga tgagtttgcg atcgcactgt tcatggcaga taacactttc tcgtggaccg
     3121 aacccatact tcgctgattc atttgccagc gtgtctgcgg gttatatgag aatcgcttaa
     3181 gtagttggtc aagctgctca agttcttctt cactgacata ctttttagcc tcaccaataa
     3241 tgccagggag aatattgccg ttaggattaa acgctcgcga aaggaagagt ttcagcgccc
     3301 ccatgccctc cgatgcttca atatcaccaa taacccggtt aacaatggcc gcactcttcg
     3361 gattagcatc cgccaacgct ctggctacga tttgcaggga cgatacgacc tcacgctgca
     3421 tatcagtcct gatctcatca ataaactctg gcgttatgcc gtgctcttta aggatatccc
     3481 ggccttccgc cgttacccca tcgatatcac cgacatgttt attaacacga ctttgcaatg
     3541 ccttaaatgc cttcagaatt ccacgggcat catccgcttt actaacggcc ttcctgaatg
     3601 ctggcaagaa gtctgagtta acctcatttt gttgatcggc ccactgaatg gaggcttctt
     3661 tcatctcgtc cagagtcaga tcacccaacg cggtatggtc tgtgaatatg agcgacaacc
     3721 tctgaaccat ttctgccaat ggtgatgccg aatgcgccgc gctaaggaat gctttcaccc
     3781 tggttgggcg aatggaaaac cagtcaatag ctggtggcat atctccgttt tttatcgcct
     3841 gcgctatctc gtcaaagcca tcgcgcccaa gggaagatgc gtgatttaac aagccgcgaa
     3901 gtaacgaatt gctgataccg aataatcggc accatttttt cacgtcggca acaggcattc
     3961 gaacaaaatg cgcaagcact tgaacaagct gttcgtcctg gggatctgca cgggaaagca
     4021 gcctgatcag atgaataatg tctttgatgc cggatgcccg atgtaatagc aagctggtat
     4081 atggagcaac accgttgtaa ctaccgccag aagctgactc gaaaagaccg ccagatatcc
     4141 cttgcatacc ttcgttttcc agttcctgag atacttggcg aaggatatcc tgtaacgaca
     4201 catcgccgcc gccaaacata tccccgagcg cctggccctg gtgctgtaac tcatcattga
     4261 tacgttgcgc catcaactta aaggcggtgg ccatacgctt cgcgctacgg ttattcgcga
     4321 cgatgaacaa cgcgagtgct ttcacttccg gggccgtttc gctgaacata tccccctgag
     4381 caataacatc ggtaatatgc tggcctgact ccttcgattg ccttaccagg tctaccgcat
     4441 ctttcaatgc cgccagcgcc tttttatcga ggctatccgc tgtctcaatg ccatcaacaa
     4501 tagttgtcac agcctgcttg tgcgcttctc ctgataaagc ctgcatctgg acaaaatcat
     4561 tggctgccgc attaagcgcc gtcagaacat tacgcatatc cggatcaggt tcttctgcaa
     4621 ccatccttac caggcgcgca tccttatatg ccttggcaaa gatcgcgttt tgtatacggt
     4681 caacaagttg ccgtgttggt cgcccatctt cagttacaag gccagccgcc tgtgtggcac
     4741 caacttgcgt cataaatccg cgaataaacg cgtcattact gcggctaagc agatctccgc
     4801 tttctgacgg gttaaaaagc gccatcatcg ccggtgttat gctgtcggca tcaacaaaag
     4861 ccttttcact ggctgccatt tcctgaagat cagaaatatt tgagtccttg gcaaactgaa
     4921 cgcggtcaac cttagttaac cggcggcgca ccagtaccgg agccgtcatt gattcaacct
     4981 tttcaggtcg tatgccgaat tcggtcgcat gatcaatcag gtactcacga taccgatccg
     5041 cattgccgtc ctgataggct ttgatgatcc ccatggtccg tccattacct gactcaacgg
     5101 cattgtcctc accaattatc ggcgcgccat ggctggataa accggaatcg gtaagctgag
     5161 caggccgcaa atctttggat atctggttaa cctgaagaag gctggatgcg cgggtccggt
     5221 cgcgcggctg aagttcctgg ggatagtctg gattaatttt cccatccaga gtattggata
     5281 ccaaaagaac tgaggcatcg acgatatcaa acgctgtttt tacctcgtct cccttcgctg
     5341 tcaccacata cgaaacccgc ccataatcgg gcaggttctt tagcagctcg atcagcgttt
     5401 ctatgctggt ggccattacc acctgatcgc ttaagctcat ccctgttacg ccttatgctg
     5461 cctctttaat gttggcggct atccatgccg ccgtgtgctg tttaacctgg tccaggtcga
     5521 tgtatgtgcc aacatattga ctcaagtcct gcaaggtacc gataaatgca tctgtgctct
     5581 gatcgacgaa tttatcagcc aggaaatcag caaccagttt tggcacacca tcatgtaccg
     5641 aaggttgttt ttcctcgcca ctactgccgc cggacgcgcc gtaccccatc tgttgcatga
     5701 tctggtcaat ttcatcgctg atatccagca actccatgcc actcgcggta gccgctttgg
     5761 acatcagagc atccaactta tcgctgaggt ccattaactc aatcgctgat agtgtcatgc
     5821 cgctaccccc gctttctgga ttgctaccag cagatcagcc aggtggcgag cagcgccatt
     5881 aaccagctct tcgttttcct caaaacgtcc ggcagcctga agggctgcaa tcgcttcccg
     5941 gacattgccc cgggcgttac ggatctccgc catgtcagtg ctttgcatat ccatcacgtt
     6001 attgagatat tcaatggctt tattagcctc tgcatctgct tcgctaaccg tttcatcagg
     6061 ctgtgccggg gccggttctg gctgagtaat ctcaccgact tcagcctgca atgcattgat
     6121 catgctctgc accattttct cggtgccagc gccccccgga aacgcaatat tggggaaagt
     6181 tttttgaaac tgagtcttca gcattacgcg gaactcgtct ggtgagctgg tggccagctc
     6241 cagagctttt tgtgcatatt tgccaaacgg accattagta agtgtcttcg ccaggaagtc
     6301 gaaagaatcc tcgcgaggca ataacttcag gtcgtactca ctcatttgct gatcagaaag
     6361 cggggtatcg taagtagcaa tgccgtagcg tgcatattca taatacgggt caccttcatc
     6421 agggcgcggc agaattgctt tgttaccttc aggtattgcg ccaggggccg ccggacgcat
     6481 ttgcagggca tatcgatatg cacctacaga gacttctggt tcaggcgaag agctactggt
     6541 atcctccgct ggttcaggtt cgacgttttc cggtttatgt tcttctggtt ggaccaggta
     6601 ttccgataca ttacccgctt tataggcttt aaacagcttg ccgatcgcat ctgccatgtc
     6661 cacaccctgt atggatttag ccttgatcat gtacacgctg ccatccgaat cggttaactg
     6721 gatataccct tcaccgtcct caatgaattg cttcattgat gcaccattac tgagcgtcac
     6781 ttccccgttc atatgcatac gatttttgat acttgcaagg cgatccgtca tcgcgcgaga
     6841 gtgtccacca gtcatccccg caggagcaat ggtttcgcgc ccaccagtgc gattgagctg
     6901 atcaatctcc gtctgcaaac gctcattctc ttcataaaga gaatccgctt ccgatgcaac
     6961 agcgttaatt ttctgctcca gatctacctt ctgcccttct accgctgcca cctgatccgc
     7021 gaggtcgctc atggcatcct ctttctggtc actgtcagcc tgtagttggg ttatttcatc
     7081 aaccagggct tttttcttct tctgcgcacg ctggaatttt gccgagtttt tctctgcaag
     7141 gttggcaagt ttcatggtga cctgcgccag cgtcatatca cgtccactca tcggagcaac
     7201 ggtgtgagta acgtcttttt tattcagtaa gaactggaaa gcaaccagcg tatcgctatt
     7261 ggtgatccgg ttttccgctg tcgggctatg aaacagaatg ctgatagtct gaccatcact
     7321 gagcggaata atggctggca ggaccggcag cccgttaacg ttccgtgccc ggccaatttc
     7381 agcaccgccg atcgcgcgcg cgccgctctg ggccacattc cccgttttat cactccccgc
     7441 agagattccg gtgccattca gcttctggtt caatgcccgg acaaatgcct gcatggtccg
     7501 gtgtaactgc aaacgagtag aactaatcgc ctccagtaaa tccgtagcac accagtggat
     7561 cggcgtgtca tagaagaacg tagcctcgat ttcctccagg gtgttggatt ccgtcatcag
     7621 atagcggtcc tcaccggcca ttaatgcgcg atattcatca tcagtcactg gcgggggaag
     7681 cacgtcaagc ccaggcttga tcgtcacccc tttattgata ttgaactgtt ccatgttaat
     7741 ttcctgcttt cagttgctta agacgacgtt tgagttcgcc attccgtgcc ttttcgttat
     7801 tgagtcggcc cgtctcctta tccagcttcg cccgcaaatc agtgatctgc tgttgattga
     7861 aagacaccga gttctgcgct gatttataag cggcaaccac ctgagcattc cgctgttttg
     7921 cttcttgcag gcgctgaaag ttggatttaa ctgccggttt cttgtctacc ggattggcta
     7981 cacgcttcgc tttggcgatc agtgatttct ggaattttgc ggagtttttg cgggccgcct
     8041 gtcccatgac ggtaccaagc gtcttgatat ccggcgactg agcgttagga atagcttttc
     8101 cattcagttt cacagacgat atatcgccag tatcgtttac ctgtatggca agaatttgtc
     8161 cgtcgttaag aaccagcttt gcggttttaa ctttaacgcc atctttcgtt gttgcgcggt
     8221 tgctggagtc aacctcaatt accgtaaccc cggttttatt gatcgccgcg ataagggatt
     8281 tcagcccctt ttcattaacc tggtcaaaat cgaccgttgc atacttattt ttcgtcatct
     8341 gacacatcct gtgcgagatt tattacgtaa cttctgcgga tttgctgagt aacagggaaa
     8401 atccgataca acgggttaat gaacgagtcg ccatgcgtaa ccatgacgtt gaaatgccac
     8461 agtcgctctc ctttacccat atattcagtg ggtatgtaca accattcact gttttcgccc
     8521 tgttcagccg acgtcaaaca acgttgttcg ccttcaatca ctgtcgttgg cttctgaaca
     8581 tcgcggatcc aatatctgac cgttgcgccg cgcaaaaacg ggaatttaga ccggtatttg
     8641 aacggcaccc ggatgaaacc cggtttaatt tccacatcac caagttctaa atgcgtgatg
     8701 tccttgcgtt ttagcaaata gcgatcggct aaggctaacg caagaacgca tacaccccag
     8761 ccaatcattt cccgcctccc tttttcacca aacttgtaag aacattcaga atgctatcga
     8821 tattcactcg tttcatccct gaaatcacct catgaccgtt attgctggct atcgttacca
     8881 ttaagtacgt aattgataac tcccagccct cgtgttgccc caataggtac gccaccgcgc
     8941 cagctgtcac tgcaacaaag atctccgtaa ccaatcccaa caaattgcca gactggcgac
     9001 cgtctcggac atccatcagg aacgtgccta tcccaccaat tactgaaagc aggagcgcaa
     9061 tagcaactgg agctaattcc tgtgtgtcaa gcacaagttc cctcctacgt tgtcaggagg
     9121 taatggtatg caaagtaact tctcaaccgg tcatgcaaag acaacaagcg gaaaaagaaa
     9181 agcaaagcct gctacaactc gtcagggata aaactcagct ctgggactca cagttacggc
     9241 taggcattat ttccgttcag ggtaaacaaa aattaaccga gtggattctc tatgcgcaga
     9301 aagtcgaatc cacggatacc tccatccttc cagtaacgtt tcccgaaaag ccggaatgag
     9361 gaaaggcccg ggatcgggcc ttaattaata tctataatcc caacaaataa gggctaacaa
     9421 ttttgttaat tttctctgga tataatgcat gtgctttaat atacctgaag ttatattaaa
     9481 gagcgtcatg taaaaattaa atcaatcaat taaatctatt ttcaatagac aaatcaaaat
     9541 ttttaacaga tatgtataga aatggcaagc cataaattaa taatgacatc cttaataagc
     9601 ccaaagaaac actgaataga taaaatataa attattgcat cccaaaatac agaaagtagt
     9661 ggcaatcttg caacttatta tcaatacaat attgacatcc ctttagtgct cagtttaaaa
     9721 ttaatatttg tgtttacatt gtgcgttggt ttagtttagc aggaacgcag taacacactg
     9781 atgct
//
LOCUS       DAZKHC020000136         9580 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZKHC020000136 DAZKHC020000000
VERSION     DAZKHC020000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.136"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..535
                     /locus_tag="ACHOSM_RS20975"
                     /old_locus_tag="pgaptmp_004195"
     CDS             <1..535
                     /locus_tag="ACHOSM_RS20975"
                     /old_locus_tag="pgaptmp_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HGU4560570.1"
                     /translation="RELVGVMQWGYALNPNSGARVVTGTQNREYMELNRLWLHDCMPR
                     NSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVGSHESIFYELD
                     GEWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRARKRLNTKLFKV
                     QPYPKPQTVKTGLKESE"
     gene            complement(659..2023)
                     /gene="tdcG"
                     /locus_tag="ACHOSM_RS20980"
                     /old_locus_tag="pgaptmp_004196"
     CDS             complement(659..2023)
                     /gene="tdcG"
                     /locus_tag="ACHOSM_RS20980"
                     /old_locus_tag="pgaptmp_004196"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU4560571.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIVRYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCV"
     gene            complement(2095..2484)
                     /locus_tag="ACHOSM_RS20985"
                     /old_locus_tag="pgaptmp_004197"
     CDS             complement(2095..2484)
                     /locus_tag="ACHOSM_RS20985"
                     /old_locus_tag="pgaptmp_004197"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HGU4560572.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(2498..4792)
                     /gene="tdcE"
                     /locus_tag="ACHOSM_RS20990"
                     /old_locus_tag="pgaptmp_004198"
     CDS             complement(2498..4792)
                     /gene="tdcE"
                     /locus_tag="ACHOSM_RS20990"
                     /old_locus_tag="pgaptmp_004198"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HGU4560573.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFYVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4826..6034)
                     /gene="tdcD"
                     /locus_tag="ACHOSM_RS20995"
                     /old_locus_tag="pgaptmp_004199"
     CDS             complement(4826..6034)
                     /gene="tdcD"
                     /locus_tag="ACHOSM_RS20995"
                     /old_locus_tag="pgaptmp_004199"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HGU4560574.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESVQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(6060..7391)
                     /gene="tdcC"
                     /locus_tag="ACHOSM_RS21000"
                     /old_locus_tag="pgaptmp_004200"
     CDS             complement(6060..7391)
                     /gene="tdcC"
                     /locus_tag="ACHOSM_RS21000"
                     /old_locus_tag="pgaptmp_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HGU4560575.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRSLARLCLSGSKPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNLFVTVIGLLTILNIVYKLF"
     gene            complement(7413..8402)
                     /gene="tdcB"
                     /locus_tag="ACHOSM_RS21005"
                     /old_locus_tag="pgaptmp_004201"
     CDS             complement(7413..8402)
                     /gene="tdcB"
                     /locus_tag="ACHOSM_RS21005"
                     /old_locus_tag="pgaptmp_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HGU4560576.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(8501..9439)
                     /gene="tdcA"
                     /locus_tag="ACHOSM_RS21010"
                     /old_locus_tag="pgaptmp_004202"
     CDS             complement(8501..9439)
                     /gene="tdcA"
                     /locus_tag="ACHOSM_RS21010"
                     /old_locus_tag="pgaptmp_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HGU4560577.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         2537 a         2467 c         2231 g         2345 t
ORIGIN      
        1 acgggaactt gttggcgtaa tgcaatgggg ttacgccctt aacccaaaca gtggtgcacg
       61 cgtcgtaacg ggtacgcaga accgagaata tatggagctt aaccggctat ggctgcatga
      121 ctgtatgccg agaaactccg aatcaagggc aatcagttac gcgctcaagc taatcaggca
      181 gctttatccg caggtacagt gggttcagtc gtttgcagat gagcgttgtg gctgtcttgg
      241 tgtcgtgtat caggcaagca attttgatta tgtaggcagc catgaatcaa tattctatga
      301 acttgatggc gagtggtatc acgagatttg caggaatgcc attaaacgag gcggacaacg
      361 aggtgaacat ctgagggcta atattgacag agctagtgtt cataagttcc gacagtttcg
      421 ttacatccgg tttctgaaca agagggccag aaaacgcctc aacacaaagc tattcaaagt
      481 ccagccttac ccaaaaccac aaacagttaa aactggtttg aaagagagtg aatgagcgct
      541 taaaaactca ttctaaaact caaaagaaaa gcgccgattt agtatcaaat aagttgcgta
      601 tcggcgtttt tcagcggtat caaatgaaat gcaaatcggt atcaaaaaaa tcgccgcgct
      661 atacgcagac cactttaatg gccagtcctc cgcgtgatgt ttcgcggtat ttatcgttca
      721 tatctttgcc ggtttcatac atcgtctcga tcactttatc gagtgaaaca cgcggtgccg
      781 aggtgcggcg catcgccatc cgcgcggcgt ttactgcttt cacggcatta atggcattac
      841 gttcaatgca cgggatttgt acctgtccgg caaccggatc gcaggtcagc ccaaggttat
      901 gctccatcgc gatttccgcc gcattgcata cctgcgccgg actgccgccc agtagttcag
      961 ttaaccctgc cgccgccatt gaacaggcca cgccaatctc cccctgacag ccgacctccg
     1021 cgccagagat ggaggcgttc attttataca gcgcgccaat agcccccgct gccagaaaat
     1081 agcggacaat tgaccgctcg tttaccggac gacggaactt atcgtaataa gccagcactg
     1141 ccggaataat gccgcacgca ccgttagtcg gtgccgttac cacgcgcccg ccagctgcgt
     1201 tttcttcact aaccgccagc gcgtacatgt tgatccagtc gatgacattc atcggatcgt
     1261 tagagatgtt atcgctagaa accagctgac gacgcagcgc tacggcacga cgcggcacat
     1321 tgagcggacc aggcagcacg ccttcagtgt tcatcccgcg ttcaataccg tcatgcatca
     1381 cttgccagat acgggcaaac cctgcgtcaa tttccgcttt gctgcgcagc gccagctcgt
     1441 tgtgcatcat caggccagat atagacaggc cgttgtaatc acacattttc agcagttcac
     1501 ctgctgagtg gaaatcgtaa ggtacggacg tttcgacatc gtgcgacagg ccgaagtgtt
     1561 cttcttcgac aataaacccg ccgccgacag agtaataggt tttacttaat agcgcttcct
     1621 gtcccttcca ggcagtgatc cgcattccgt tctcatggcg aggcaacatt tcgggatgga
     1681 agataatgtt ctttgctaca ggaaaatcaa caatatgcgc accagatgcc actggcagcc
     1741 gcccgctgcg cgttactaac tctataaatg cagggatctc atcaatgaca acatcctgcg
     1801 gactgtttcc tgccagtccc atgataatgg cgacatccgt ggcatggcct ttgcccgtca
     1861 gtgacaacga cccatacaga tcgaccacaa tatggctcgt cgcggttaat aagccgctac
     1921 tttccagccg atcaataaaa ctttttccgg cattcattgg ccccacggta tgcgaactgg
     1981 agggaccaat cccaattttg aaaatatcga atgcactaat catatccaca ccctcggatt
     2041 gccgttcagt gaagtggagc ggaacgacct tgcgaccgtc ccgctcacga ggctttacgc
     2101 actacgtact gcgatagctt caatttccag cttcacatct ttcggcaaac gcgcaacctg
     2161 cacacagctc cgggtcgggt aggtcgcctg atgctcatcg aagaactgct tatagacttc
     2221 attgatggtg gcaaaatcat tcagatcggt gatgaacacg gtcatcttga tgatatcgcc
     2281 cacgctcagc ccggcagcaa ccacgatcgc tttgacgttt tcgaggctta aacgcgcctg
     2341 atcttgcaca tcagccggga tctcaccggt ctgcgggcaa accggaattt gcccggaggt
     2401 gaagaccatg ctgcctaaat caacgccctg aacataaggg ccgattgcgc ctggggcacg
     2461 ttgcgtttcg ataatctttt tcatacatcc tccggcgtca gagcgcctgg gtaaaagtac
     2521 gtgaaataac atcctgttgc tgttcacggg tcagtgcgtt gaagcgcacg gcgtagccag
     2581 agacacggat tgtcaggtta ggatattttt ccgggtgctc gatggcatcc agcagcattt
     2641 cccgattcat tacgttgacg ttgaggtgtt gaccgccttc gacatccgct tcgtggtgga
     2701 aatacccatc cagcaggccg acaaggttgg ttttacgtac tggatcttct ttgcccagcg
     2761 ccgcaggaac gattgagaag gtgtacgaga tcccatcttt ggcgtaggtg aacggcagtt
     2821 tcgccaccga cgtcaatgag gccacggcac ctttgcggtc acgaccatgc atcgggttag
     2881 cgcccggcgc gaacggtgtt ccggcgcgac gaccgtccgg cgtattaccg gttttctggc
     2941 cgtacaccac gttagaagtg atagtcagaa tcgactgggt agggacggcg ttgcgatagg
     3001 ttggcagcgc tttaattttc ttcataaagc gttcaaccag gtcgcaggca atgctgtcta
     3061 cgcgctcgtc gttgttgccg tactgcggat attcaccgtc gatttcaaag tccaccgcca
     3121 ggccgttttc gtcacggatt ggtttcacgc gggcatattt gatggcagac agggagtccg
     3181 tcgccaccga caggcccgcg atgccgcatg ccatagtgcg atagacatca cgatcgtgca
     3241 gcgccatcag cgaagcttcg tagctgtact tgtcgtgcat gtagtgaatg atattcagcg
     3301 cgctgatgta ctgcaccgcc agccagtcca tgaagtgatc gaggctgtcc atcactttgt
     3361 cgtaatccag cacgtcgtcc atcagcggtg ctgttttcgg cccgacctga atcttcagct
     3421 tctcgtccac cccgccgtta attgcgtaga gcagcgtttt cgccaggtta gcgcgtgcac
     3481 caaagaactg catttgctta ccaatcacca ttgggctgac gcagcaggca atcgcgtaat
     3541 cgtcgctgtt gaagtcagta cgcatcagat cgtcattttc atactgcaag gaagaggtga
     3601 cgatcgacac ctgcgcggca tattttttga aggcaatcgg taattcttcc gaccaaagaa
     3661 tggtcaggtt aggttccggt gccggcccca tagtgtgcag ggtgtgcaaa tagcggaagg
     3721 agtttttggt caccagcgta cgaccgtcca gccccatccc gccgatcact tccgtcgccc
     3781 agattgggtc gccggagaac agcgaatcaa attccggtgt acgcaggaag cgtaccatac
     3841 ggatcttcat gatgaagtga tcgatcagtt cctgtgcctg ctgctcattg agtacgccag
     3901 ctttaaagtc gcgctcaatg tagatatcga ggaacgatgc cgtgcggccc agcgacatcg
     3961 cgccgccgtt ctgtgatttc actgccgcca gataagcgaa gtagagccac tgcaccgctt
     4021 cctgcgcatt ctgcgccggg cgagagatat caaagccata tttcgccgcc atttcctgaa
     4081 tctgcaacag cgcatgacga tgctctgcca gctcctcacg cagacggatg gtggcttcca
     4141 gatcctcgcc tttttccaga cgagactgga gatcggcaaa ttgcagttcg cgttcacgta
     4201 ccagataact gatgccatac agcgctacgc ggcgatagtc accgataatg cgcccacggc
     4261 catagccatc tggtaaaccg gtcagcacgc cagatttacg gcagcgcagc atatccggtg
     4321 agtaaacata aaatacgccc tggttatggg ttttacgcag atcggtaaac agatattcaa
     4381 attcactgtc catttcacgg ccataggcgt ggaatgaact tttaatcata ttaatgccac
     4441 cgaacgggtg tagcgcacgt ttcaacggcg catccgtttg caggccaaca attttttcca
     4501 gcggctggtt aatatatccc gcatcatgag cggtaattgt ggtggcaata ttggtatcga
     4561 aatcaaccgg cgcgtgggtt gcgttttcga tacggatacc ttccattact ttttcccaca
     4621 attccgtggt ggcaggcgtc gcttcggcga ggaaagattc atcgccttca tacggtgtat
     4681 agttatgttg aataaaatcg cggacattaa tttcgttttt ccagtccgta cctttaaagc
     4741 caagccatgc gtcggcgtac agcttatcgc tggtatcaat atctaccttc atgaaaaata
     4801 atctctctac aatacttcaa ctaaattatg caaattctgc gggcgcgtta actttgccta
     4861 aatgaatggc atccaaagca atcatttttt cttcgttagt cggaataaca gcgcaaatga
     4921 cacgcgcatt ttcactggaa acaattcgct caccacagga gttagagcga ttattcattt
     4981 ctgtatcaat ctctacgcct aatacagcca aatgttccat gaccagacga cgaattaagc
     5041 ttgaattctc tcctattccg ccggtgaata taattccatc caggcgacgt aatgaagctg
     5101 cgtgtccggc aatatgacgg gcaattcggt gaacaaaggt tttaattgcc agttgcgcgc
     5161 gttcgtgacc ttcatgccag gctttttcca gaacacgtaa atccgaagaa agaccggaaa
     5221 tacctaataa tcccgactct ttattcacta cgcgttccag gtcacccagg ctctggttgg
     5281 tttggctggc gacccaggac atcgcaccaa aatcgacatc gccactgcgg gtacccatca
     5341 tcaagccttc cagcggcgtc attcccattg aggtgtcaac actctgaccg ttgcgaactg
     5401 cgcagattga cgcgccattg ccaagatgcg ccacaaccag gccggagtca tcttccgcca
     5461 gattcagcaa agaatgtgcg cgctgggaaa catagcggtg cgacgtgccg tggaaaccat
     5521 aacggcgtac acctaactct tcataatatt tccacggcag gccgtataaa taagcttccg
     5581 gagccatcgt ctggtggaaa ctggtatcaa ataccgccac ctgagttacg cccggaaata
     5641 attgctgcac cgattcaata ccacttaaat tggcgtaatt atgcaggggt gccagtggag
     5701 aaacgcgacg gatattatca atgacctcat cggtaataat ggcggactcg gtaaaaatac
     5761 tgccgccgtg agcgatgcgg tggccaatta aggccacact gtcatttaaa ttccgttttt
     5821 ccagttcaaa tgcaattgcc ttcaatgcac cttcgtagct gtggtgagcc agcggtgctg
     5881 gctctccccc atttacggat aagaatgcat tttccgagtt aataccgtcg gcaatacctg
     5941 acattaatac ttcacagtcg ctggcatcga gtacggaaaa cttaatcgaa gacgaaccac
     6001 agttaataac caaaacaacc ggaaattcat tcatctcttt tctcatcctg agttacggat
     6061 taaaacagtt tgtatacgat gttcaggatg gtcagcagac caatcacggt aacaaacaag
     6121 ttatccagac gaccacggta tttcgccaga gacggcgctt tacggatggc atacatcggc
     6181 aacaggcaca gcagggatgc gataatcggt gcgcccatgg cttcaataag gtcgaggatg
     6241 ttcgggttgg cgtaggcaac aacccaggtg gagcccatga tgaagatcat gctgatagtg
     6301 ttcagtttac ccagcgacac tttagttttg tcgcctttat aaccgaactt cagaatcaga
     6361 ccattcaagc cttccagcgt ccccagatag tgaccgaaga aagatttgaa gatagccacg
     6421 agtgcgatga tggaagccgc atattccagt gtaatcgcga acgttgtttt ggtaccggtc
     6481 atggacgcaa agtggttagc cagataagaa agcactggaa tattctgcgc tttggcttcc
     6541 gccatgttgg ccggagacag cgtaaacagg cagctaaagg caaagaacat caccactgca
     6601 accatcagca tgctggcacg agaaatgatt tgggaacatt tacgttcggt gaagtcgcga
     6661 ccgaagtctt tctcatactc ttcacgttta gaaaccacga aggaagagac gattggcgag
     6721 aagttaaagg agaaaaccat gatggaaatc cccagccaga cagtgatcag gataccgtca
     6781 tgaccggtta acgacagcga accgaggtca acctggtcga taactgcaga gttccagtaa
     6841 gggatcagcg acagagaaat cagcaccagg ctggcgataa acggccatac caggtagctc
     6901 atcactttaa ccatcagatc cttaccaaac cagatgacga aagccatcag cagcaacagg
     6961 aacagcgcca caaagccgcg attcagcggt gcaaatccga gctggttttc ccagaacgtc
     7021 ataaaggtat tggtaatagt aacgccataa atccacagca gtgggcaaat cgcgaagaag
     7081 tacaggaacg tgataaccac gccgccagtt ttaccaaaat gctcttccac cgtttccgta
     7141 atgttgccgg aaggcttaga gccagaaaga cacagacgcg ccagcgaccg gtggcaataa
     7201 aatgcgatgg ggtatgccaa taccaacatc agaagaatcg gaatcagtcc gccaaaacct
     7261 gcgcggatag ggaagaacag caccccggcg ccgattgccg taccaaacaa gcctaacgtc
     7321 catgtggtat ctgatttacg ccaggacgat tgttttgtct ggctcgatac aatgctatct
     7381 gaagtactca tatcctatcc tcaacgaatt aattaagcgt caacgaaacc ggtgatttga
     7441 gagacgcgag aaagatcgat attgccgccg gaaataatac tgacggtttt tctgttttga
     7501 atatattggt ctaatttacc gcttaataat gcagcacatg ccagagcgcc tgcgccttcg
     7561 gtgacgactt tattgcgctg aattaaggca atcatactgt ttctgatttc gtcttcgctg
     7621 accagcacga tgtcatcgac taattcacga acgatttcgt aagttaaatt acccggacgg
     7681 gagacatcac aaccatccgc cagggtgccg gtagttcggt gcgtggttat ttctccggag
     7741 tggaaagaag ccgccatgcc gtgaacgttt tctgactgta cgccaataac acgaatggtc
     7801 gggttaatag atttaattgc caccgcaata ccagcaatta aaccaccacc accaattggc
     7861 acaatcacgt tatcgacatc atagagatct tccataattt ccagaccaat cgttccctgg
     7921 ccagcaatca ctttcggatc atcgtaaggt gggataaaaa tacgaccttc catttcgaca
     7981 atttcgctca ctttagcgat agtgtcgttg aagttatcac catgcagaac gacttctgcg
     8041 gagtagtcgc acgttgccgc tactttggat tttggcgcac cttttggcat caccacttta
     8101 ccgtcgatgc ccagcatcgc gcaggagagc gaaacccctt gcgcatggtt gcccgcagaa
     8161 caagccacca cgcctttgcg tttttccgca tcggtcagtg aacttaattt attaaatgcg
     8221 ccacgaattt taaatgaacc cgtacgctgc atattttcaa acttcaggaa tatttcacct
     8281 ttgcaacgtt cactaaaata gttggagcga ggcatgcctg ttttataaat tcgcccagcc
     8341 agtcgttgtt tcgcttcaat aatatcatca atagcaaccg gcagatcgta tgtaatatgc
     8401 attataaaac ctcttcgcct gaattaaata tgtaggtaac cgtaaccgac acctgcaaaa
     8461 cagacaggtg gattatttat gtttaaataa aaacaaataa ctaaccaact tctattaatt
     8521 gccttcgtct acacccatta taagatgaat actctttggc taattccacc aaaaccgatg
     8581 ctgctttttt aatacgataa tttttcgacc ataccgcggc atattgtgcc acaggtaatg
     8641 tttcttcaac cggaatagta ataaattgat ttgaaccaaa aggtgacgtc atatcacaag
     8701 gaattacagt taagaaatca gcattgagaa caagattata aattgtcacg actgagtcgg
     8761 ttttaacgat gttttcaata ctgatgccat ttctttgtaa cgtagtaaga agctcgctgt
     8821 agtaccccat attggtttgt ggcaacaccc actgttcgtt cttcaacgac tccagcgtgg
     8881 tggtgccggt gcatgttcgg gacttactag ctaccagcac aaactcggac tcgaacagcg
     8941 gctcaacatg taaatcctga agcttcattt ctgcacttaa cgtaccaatc gcaaaatcca
     9001 gtcgaccgtc gcggattgcc ggtaagaacg aagacagttg cgcttcatac atagaaacct
     9061 gcgctttcgg gaacacctct ttgaacttgt tgatcatccc tgacataaaa gtaaaaccaa
     9121 tcaatgaagg aaaaccaaat gagacttcca ccaccgcctc agaagacata ccgcttatct
     9181 cattaaccat atttttcatt tcacgggtaa tggattcgga acgggagagt aacaattgac
     9241 cggcaggtgt taatgttaca ccggtatttt tccgcaccac taattccaca ccaaaataat
     9301 cttcaatatc gttaatgatt ttactgacgg ccggttgagt taaccctaat tcttttgcag
     9361 ccgagccgat agagccactt ctaatgactt cctgaaagac taccaggtgc tgcgttttcg
     9421 gaagaagaat agtgctcata acgacctacg ttaattacct cattgacggc atgaagtgta
     9481 tcaaaatgaa atgaacagga tatgtgcgac cactcacaaa ttaactttca ttactttcca
     9541 gggtatcgtt attacaatta aataccttta aacatcaaca
//
LOCUS       DAZKHC020000137         9561 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZKHC020000137 DAZKHC020000000
VERSION     DAZKHC020000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.137"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..71
                     /locus_tag="ACHOSM_RS21015"
                     /old_locus_tag="pgaptmp_004203"
     CDS             <1..71
                     /locus_tag="ACHOSM_RS21015"
                     /old_locus_tag="pgaptmp_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560578.1"
                     /translation="KMRKAAMDRNYLASVLAGSGLS"
     gene            511..1404
                     /locus_tag="ACHOSM_RS21020"
                     /old_locus_tag="pgaptmp_004204"
     CDS             511..1404
                     /locus_tag="ACHOSM_RS21020"
                     /old_locus_tag="pgaptmp_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4560579.1"
                     /translation="MLKENFNELQIFLLVARERSFTKAAGKLGVTQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLKKYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKALRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(1436..2425)
                     /locus_tag="ACHOSM_RS21025"
                     /old_locus_tag="pgaptmp_004205"
     CDS             complement(1436..2425)
                     /locus_tag="ACHOSM_RS21025"
                     /old_locus_tag="pgaptmp_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGU4560580.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGSIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2452..3303)
                     /locus_tag="ACHOSM_RS21030"
                     /old_locus_tag="pgaptmp_004206"
     CDS             complement(2452..3303)
                     /locus_tag="ACHOSM_RS21030"
                     /old_locus_tag="pgaptmp_004206"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGU4560581.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINTGYRLID
                     TAASYQNETQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLHAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            3869..8122
                     /gene="fdeC"
                     /locus_tag="ACHOSM_RS21035"
                     /old_locus_tag="pgaptmp_004207"
     CDS             3869..8122
                     /gene="fdeC"
                     /locus_tag="ACHOSM_RS21035"
                     /old_locus_tag="pgaptmp_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HGU4560582.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVSKSTLSVNELSGDVVADGQQSHTLTLTAVDTDGNPVTGEASRLR
                     FVPQDTNGVTIGTISEIKPGVYSATVSSNRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGMKPDAPVFSGASNTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIGDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLNLPQGVTSKTGNTVTTNAAGKADIELISTVAGELEIAAAVKNSQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSDTQTITFVADKATATVSGIE
                     VIGNYALADGKAKQTYKVTVTDANNNLVKDSDVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEEDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
     gene            complement(8243..9100)
                     /locus_tag="ACHOSM_RS21040"
                     /old_locus_tag="pgaptmp_004208"
     CDS             complement(8243..9100)
                     /locus_tag="ACHOSM_RS21040"
                     /old_locus_tag="pgaptmp_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4560583.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            9349..>9561
                     /locus_tag="ACHOSM_RS21045"
                     /old_locus_tag="pgaptmp_004209"
     CDS             9349..>9561
                     /locus_tag="ACHOSM_RS21045"
                     /old_locus_tag="pgaptmp_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGU4560584.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRD"
BASE COUNT         2508 a         2191 c         2479 g         2383 t
ORIGIN      
        1 gtaagatgcg aaaagcagcc atggacagaa actatctcgc gtcagtcctt gcggggagcg
       61 ggctttcgta atcttgccct ggtaagtgcc cttatataaa taacgagttt ggtcaaccaa
      121 ttttttgaca tgtatcacaa atttgaatag atgtattaca tcggctatct tttattgcac
      181 caacgtcatt gatatatgtc gcctgaagtc agttccggga atgagtctga tctcaagact
      241 ggcccagtcc gggcgttgat tggcgctgag gagcatatcg catctcatca taatgtcgta
      301 tctcctttaa tgttgtgtgt cagaatatcg gtaagaagaa tggcgcaatc gtgagaatac
      361 gtttattcat aataaatatc ctgaaagttt agaattattt tcgatacagc cagattatca
      421 ttgttactaa taatgtggtt aaaataatag catctatgac cccccttcat gagtgtctgt
      481 caagaggatg cttctacaag gaaatcgcca atgctcaaag aaaacttcaa cgaactgcaa
      541 atctttcttt tggtggcaag agagcgaagt tttaccaaag cagcgggcaa actcggcgtc
      601 actcagtctg cacttagcca tgcaatgaag gctctggagg aaaggctcaa tatccgcctc
      661 ctgacacgga ctacccgcag tgtggctccc acagaagcgg gggagagaat cattgcctgc
      721 cttgaaccgc gtattgacga gcttgaacag gaactggagt cacttattca actgaacggc
      781 acgccctccg ggaatattcg tttatctgcc ggggaacatg ccgcgcgaag tctggtctgg
      841 ccgaagctaa aaccttttct caaaaagtat cccgaaatca atgtcgaact ggtggtggat
      901 aacggttttg tcgatattgt tgaaggccgt tttgatgccg ggatacgact gggtgaaaat
      961 gttgataaag atatggttgc ggtcaggatt ggaccagata tgcggatggc tgtggtgggt
     1021 gcaccggcgt attttgctgc aaaccctgcg cctgaaacgc ctcacgagct acaaaaccat
     1081 cgctgcatca acatgcgttt gccaactgcc ggtggacttt accactggga attcgagcga
     1141 gaagggaaag cgcttcgcgt taaggttgat gggcagctga cgtgtagtcc attgccagaa
     1201 agaattgatg ccgcgttatc agggtttggg atcgcttgtg tccctgaaga catggttcag
     1261 gagtatataa agtcaggcaa gcttattcag gtgttacagg agtggtgtcc aacttttccc
     1321 ggatattatc tttactaccc cagccgcaag cagcatccgc cagcttttgc gctgttgatc
     1381 gatgcgcttc gctacaccga ataacgggtc ctttgttcct ggacccgttt gggtattagc
     1441 ggcctacgcg tgcctggtgt tcaggcgagt aacgttcacc gacgatttta attgtttcca
     1501 gcgcctcggt tatctgctgc gtatctttct gcgaaagaac aatgtcagca gcggccaggt
     1561 tttcttccag ccggtgcagt ttagtggtgc cagggatggg aacaatccac ggcttttgtg
     1621 ctaacagcca tgccagtgcg atttgtgcag acgttacccc tttctccgca gccagttcac
     1681 ctaataaggt gaccagtttt tcattagctt caatggcttg tgctgcgaaa cgcggtacgg
     1741 tgctgcggta atcatctttg ccgaaagtgg ttcccggctt aatcgacccg gtcaggaaac
     1801 ctttacctag tgggctgaag gggacaaaac caatccccag ttcctccagc aacggcagaa
     1861 tttcctgttc aggctcacgc caccacatgg aatattcgct ttgcagggcg gtgacaggtt
     1921 gtaccgcatg cgcacgacga atggtttgtg ctcccgcttc agacaggccg aaatgtttga
     1981 ccttaccttc tgcgatgagg tctttcaccg ttcctgcaac atcttcaata ggaacatccg
     2041 gatcaacacg gtgttggtac agcaggtcaa taacatctgt cttcagacga cgtaatgatc
     2101 cttcaacggc ttcacggata tgttccggac ggctgtttaa aatctgctgc ttattatcat
     2161 cgccgaaggt aaaaccgaat ttagtggcga tcaccacgcg gtcacgaaac ggttttaagg
     2221 cttcgccgac cacttcttca ttcagatacg gaccatacac ttcggcggta tcgaagaagg
     2281 tgacgccacg ttcaaccgca gcacgaataa gttcgatagc ctgacgggta tcggtcgccg
     2341 ggccgtagcc gtggcttaag cccatgcaac cgagcccaag ggcggagact tcgagtccgg
     2401 atttacccag ataacgtttt tgcattgaat gaatacctct tttatcgcgg cttaaacatc
     2461 aagtttgcgg ccagtcagcc attccaccat tgcgggatcg cggtgagaga agaacgcgct
     2521 ggttgcggta tccagggcgg taatttgcag catatcttca gggctgagtt caaaatcgag
     2581 aatgttgatg ttctcttcca tgcgttcttt gcgtactgat tttgcaagcg aaacaatgcc
     2641 tcgttggtag atccagcgca gaacaacctg gcctacgctt ttgccatact tctcgccaat
     2701 tgccgtgaga acaggatgct ggaacagacc atttttccct tccgcaaacg gtgcccaggc
     2761 ttctggctga ataccacgac tttgcatcca cggaacggcg tgtagctgct gattgaaagg
     2821 gttaacttca acctggttta ccgctggaac gacgttatta aaggcgataa ggtcagccag
     2881 tcggtcagga tggaagttac tgacgccaat agcgcgaatt ttaccggcct gttgcagctc
     2941 ttccatggca cgccaggctc cgtggacatc gccatatggc tggtgaatca agtacaggtc
     3001 aacgtaatca agttgcagtc gattcagaga gcgttcgaac tgggctttgg ccccttcgta
     3061 actggtatcc tgcaaccaga gtttcgtggt gacaaagagt tcgttacggg cgataccgct
     3121 ttgtttcagt gcgttcccga cctgggtttc attttgatag gaggcggcgg tgtcaatcag
     3181 tcgataaccg gtgttaatag cgtcaatgac cgctctttcg cattcagcgg catcggtcat
     3241 ctgaaataca ccaaagccca acaggggcat ttcaataccg ttgttcagtt ttactttttg
     3301 catggtgtta tccttccgtt ctcgttcatg agaagcataa cgtaaagaga agagctcgat
     3361 tagacggcag gatttgtcag gggttatgaa cgaaattcat aaatctgttt gagtgttgcg
     3421 atgggtagtg caagttcgat atctccgcaa tttacagtcc gatgaaggaa aatgaatatc
     3481 cataaaaaat atatcggttt atcctggcgt atatacctac ttcgacgtat ttccaatagt
     3541 tgctaattaa aggcaggtca ttattattca ctctgaataa tgaattattc actgtccgca
     3601 gagtaagaaa tataacttag gtatctattt aatgacttgc acaaaaagct aaattttcac
     3661 ccataaataa aaatataatc ccgcgcccaa ccacctgatg agtggctaca ggcactggat
     3721 atattaggtg gcggtgtact ttcttacata aaggtatttc cttttctgcg gaaaaggaaa
     3781 ccgggaaatc cccggttttt ctgacaagca gacgccatta tttgtgtctg cctatgttcg
     3841 ttaattcgtt catcaggaaa ttatctcaat gtcacgttat aaaacaggtc ataaacaacc
     3901 acgatttcgt tattcagttc tggcccgctg cgtggcgtgg gcaaatatct ctgttcaggt
     3961 tctttttcca ctcgctgtca cctttacccc agtaatggcg gcacgtgcgc agcatgcggt
     4021 tcagccacgg ttgagcatgg gaaatactac ggtaactgct gataataacg tggagaaaaa
     4081 tgtcgcgtcg tttgccgcaa atgccgggac atttttaagc agtcagccag atagcgatgc
     4141 gacacgtaat tttattaccg gaatggccac cgctaaagct aaccaggaaa tacaggagtg
     4201 gctcgggaaa tatggtacag cgcgcgtcaa actgaatgtc gataaagatt tctcgctgaa
     4261 ggattcttcg ctggaaatgc tttatccgat ttatgatacg ccgacaaata tgttgttcac
     4321 tcagggggca atacatcgta cagacgatcg tactcagtca aatattggtt ttggctggcg
     4381 tcatttttca ggaaatgact ggatggcggg ggtgaacacc tttatcgacc atgatttatc
     4441 ccgtagtcat acccgcattg gtgttggtgc ggaatactgg cgcgattatc tgaaactgag
     4501 cgccaatggt tatattcggg cttctggctg gaaaaaatcg ccggatattg aggattatca
     4561 ggaacgcccg gcgaatggtt gggatatccg cgcagagggc tatttacccg cctggccgca
     4621 gcttggcgca agcctgatgt atgagcagta ttatggcgat gaagttgggc tgtttggcaa
     4681 agataaacgc cagaaagatc cacatgcgat ttccgctgaa gtgaattaca cgcctgtgcc
     4741 tcttctgacc ctgagtgccg ggcataagca gggcaagagt ggtgagaatg acactcgctt
     4801 tggcctggaa gttaattatc ggattggcga acctctggag aaacaactcg atacagacag
     4861 cattcgcgag cgtcgaatgc tggcaggcag ccgctatgac ctggttgagc gtaataacaa
     4921 catcgttctt gagtaccgca aatctgaagt gatccgtatt gctctgcctg agcgtattga
     4981 aggcaagggc ggtcagacac tttccctcgg gcttgtggtc agcaaagcaa ctcacggact
     5041 gaaaaatgtg cagtgggaag cgccgtcatt actggctgaa ggtggcaaaa ttaccggtca
     5101 gggtagtcag tggcaagtaa cgctcccggc ttatcgtcca ggcaaagaca attattatgc
     5161 gatttctgcg gttgcctacg ataacaaagg caatacctca aaacgcgtgc agacagaggt
     5221 ggtcattacc ggagctggta tgagcgccga tcgcacggcg ttaacgcttg acggtcagag
     5281 ccgtattcaa atgcttgcta acggtaatga gcaaaaaccg ctggtgctgt ctctgcgcga
     5341 cgccgagggc cagccagtca cgggcatgaa agatcagatc aagactgaac taactttcaa
     5401 accggctgga aatattgtga ctcgttccct gaaggccact aaatcacagg caaagccaac
     5461 actgggtgag ttcactgaaa ctgaagctgg ggtgtatcag tctgtcttca ctaccggaac
     5521 gcaatcaggt gaggcaacga ttactgttag cgttgatggc atgagcaaaa ccgtcactgc
     5581 agaattgcgc gccacgatga tggatgtgtc gaagtctact ctgagcgtga atgaactctc
     5641 aggagatgtg gttgctgatg gtcagcaatc ccacacgctg acgcttactg cggtggatac
     5701 tgatggtaac ccggtgacgg gagaggccag ccgcttgcga tttgttccgc aagacactaa
     5761 tggtgtcacc attggtacaa tttcggagat aaaaccaggc gtttacagcg ctacggtttc
     5821 ttcgaaccgt gccggaaacg ttgttgtgcg tgctttcagc gagcagtatc agctgggcac
     5881 attacaacaa acgctgaagt ttgttgccgg gccgcttgat gcagcacatt cgtccatcac
     5941 cctgaatcct gataaaccgg tggttggggg tacagttacg gcaatctgga cggtaaaaga
     6001 tgcctatgac aaccctgtga ccagcctcac gccggaagcg ccgtcattag cgggggccgc
     6061 tgctgtaggt tctacggcat cgggctggac aaataatggt gatgggacgt ggactgcgca
     6121 gattactctc ggctctacgg cgggtgaatt agaagttatg ccgaagctaa atggacagga
     6181 tgcggcagca aatgcggcaa aagtaaccgt ggtggctgat gcgttatctt caaaccagtc
     6241 gaaagtctct gtcgcagaag atcacgtaaa agccggcgaa agtacaaccg tgacgctggt
     6301 ggcgaaagat gcgcatggca acgctatcag tggtctttcg ttgtcggcaa gtttgacggg
     6361 gaccgcctct gaaggggcga ccgtttccag ttggaccgaa aaaggtgacg gttcctatgt
     6421 tgctacgtta actacaggcg gaaagacggg cgagcttcgt gtcatgccgc tattcaacgg
     6481 ccagccagca gccaccgaag ccgcgcagtt gacggtcatc gccggagaga tgtcatcagc
     6541 gaactctacg cttgttgcgg acaataaggc tccgaccgtc aaaacgacga cggaactcac
     6601 cttcaccgtg aaggatgcgt acgggaaccc ggtcaccggg atgaagccag atgcaccagt
     6661 gtttagcggt gcctccaaca cggggagtga gcgtccttca gcaggaaact ggacagagaa
     6721 aggtaatggg gtctacgtgt cgaccttaac gctgggatct gccgcgggtc agttgtctgt
     6781 gatgccgcga gtgaacggcc aaaatgccgt tgctcagcca ctggtgctga atgttgcagg
     6841 tgacgcatct aaggctgaga ttggtgatat gacagtgaag gttaataacc aactggctaa
     6901 tggacagtct gctaaccaga taaccctgac cgtcgtggac agctatggta acccgttgca
     6961 ggggcaagaa gttacgctga atttaccgca gggtgtgacc agcaagacgg ggaatacagt
     7021 aacaaccaat gcggcaggta aagcggatat tgagttgata tcaaccgttg caggggaact
     7081 tgaaatcgcg gccgcggtga aaaactctca gaagacggtc acggtgaaat tcaacgcgga
     7141 tgccagcacc ggtcaggcaa acctgcaggt agacaccgcc gttcaaaaag tagcaaacgg
     7201 caaagatgcc tttacgctga cggcgaccgt tgaggataaa aatggtaacc ctgttccagg
     7261 gagcctggtg acctttaatc tgccccgggg tgtcaagccg cttacaggcg ataatgtctg
     7321 ggtgaaagcc aacgatgagg ggaaagcaga gttgcaggtg gtttcagtga ctgccggaac
     7381 gtatgagatc acggcatcgg cggggaatag ccagccttcg gatacgcaga ctataacgtt
     7441 tgtagccgat aaggctaccg caaccgtctc cggtattgag gtgattggca actatgcgct
     7501 ggcggacggc aaagccaaac aaacgtataa agttacggtg actgatgcca ataacaattt
     7561 ggtgaaagat agcgacgtga cgctgactgc cagcccggct tcgttaaacc tggaaccgaa
     7621 tggcactgcg acaacgaatg agcaagggca ggctattttc accgctacca ctactgttgc
     7681 ggcgacatac acactcaagg cgcaagtgag tcagaccaac ggtcaggtat caacgaaaac
     7741 tgccgaatct aaattcgttg cggatgataa aaacgcggta ctcaccgcat catctgatat
     7801 gcaatctctg gtggcggatg ggaaatcgac tgcgaagctg gaggtgacac tgatgtcggc
     7861 aaataacccc gttggcggga atatgtgggt cgacattcag acgccggaag gggtgacgga
     7921 ggaggattat cagttcctgc cgtcgaaaaa tgaccatttc gtgagcggaa aaatcacgcg
     7981 taaatttagt accagcaagc ctggtgtcta tacgttcaca tttaacgccc tgacgtatgg
     8041 cggatacgaa atgaagccag tgacggtgac cattaccgcg gtggatgccg atacggcaaa
     8101 ggacgaggag gcgatgaaat aatattaaga atatgaaagt aacattctaa taaacaataa
     8161 tggcgttgac atcttcaacg ccattattta ttgaatttcc tgacagtagg ctcgttataa
     8221 attcatttag atatagcgaa aactaaaccg gacgaacggg gataaaaatt tcgcaaacaa
     8281 catcattgct atccttttct acgaaatctt cattgtaata aaaccgctca atatcactgc
     8341 cgttcactct ggctaacccc ctggcgggta attccatcag atagaggttt cgcgcccatt
     8401 cagggtagtg ctcccactca ccttcatagc ggaaagaggc atacagtcct cggttaattg
     8461 tccactcttt cggcatctct gaatcggttt gtttgcatac cgtaccggca aaagtttcaa
     8521 ccgtgagcaa atcgaccgat tttgttgacg gttcaaacgt ggagacgaaa tagatatccg
     8581 tcttaaaatt attcatacat ctggctaaat gatgacgtcg cagtttgact tcttcatccg
     8641 gtgatttatt cgacacttca atgtcatatt taaatgagtt gccaaaaata ggtgtctcag
     8701 gaaaattgac taatctacac tccgtctttt gctgttgacg tattaaaaaa gaagggaaga
     8761 tatttgcgag atcccaataa tcacggtggc ggtattcacg gggagagcag ccgaataact
     8821 ttttaaattc gcggctgaat gactgctgtg aatcaaaatg caaagagagt gcaatatcaa
     8881 gcatagattt cgcggtgagc cggacaagaa tagcggcacg gcaaagcctt cgtttgcgaa
     8941 tgtattctcc caaaggcaca tgcatgaaat ttcggaacag aagctggatg ttgcgtctgc
     9001 tgtagccaga tttctgtgcg atatcttcga ttgaaatagg gtgctcaaga ttgcactcaa
     9061 tccactccag gagctgctgt agaatcttct gcctgatcat cacattctcc gttaattctt
     9121 tgtcatatcg cacattttcg ttatgccctg ggccctaagt gtatcaaaaa cagcaagggc
     9181 gaaggcaaaa gcactggtag cgtctgcttc gttgtccccg aaaatagaga ggtttcagtc
     9241 ctgcattatt aatgaatttt ttgcataagt gatatcaaaa tccatgtact aatttgaggt
     9301 tacgttttaa cgtagactca ttgttcatgc ctaatggagg gactgacagt ggaattttcc
     9361 gtattaagta acaatctgaa aatgccgatg atgggatttg gtgtttttca ggttaccgat
     9421 aaaaacgtgt gcaaacagtc agtgttgaac gctatccgca caggttatcg actcatcgac
     9481 actgcagcgg tatacggtaa tgaagatgcc gtcggtgaag ccgttcgtga agctatatct
     9541 gaaggtttat gtacccgaga t
//
LOCUS       DAZKHC020000138         9516 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZKHC020000138 DAZKHC020000000
VERSION     DAZKHC020000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.138"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(390..920)
                     /gene="yodB"
                     /locus_tag="ACHOSM_RS21050"
                     /old_locus_tag="pgaptmp_004210"
     CDS             complement(390..920)
                     /gene="yodB"
                     /locus_tag="ACHOSM_RS21050"
                     /old_locus_tag="pgaptmp_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGU4560585.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(1263..1913)
                     /gene="zinT"
                     /locus_tag="ACHOSM_RS21055"
                     /old_locus_tag="pgaptmp_004211"
     CDS             complement(1263..1913)
                     /gene="zinT"
                     /locus_tag="ACHOSM_RS21055"
                     /old_locus_tag="pgaptmp_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HGU4560586.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2170..2805)
                     /gene="msrQ"
                     /locus_tag="ACHOSM_RS21060"
                     /old_locus_tag="pgaptmp_004212"
     CDS             complement(2170..2805)
                     /gene="msrQ"
                     /locus_tag="ACHOSM_RS21060"
                     /old_locus_tag="pgaptmp_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HGU4560587.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(2806..3810)
                     /gene="msrP"
                     /locus_tag="ACHOSM_RS21065"
                     /old_locus_tag="pgaptmp_004213"
     CDS             complement(2806..3810)
                     /gene="msrP"
                     /locus_tag="ACHOSM_RS21065"
                     /old_locus_tag="pgaptmp_004213"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HGU4560588.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(3919..4332)
                     /gene="hiuH"
                     /locus_tag="ACHOSM_RS21070"
                     /old_locus_tag="pgaptmp_004214"
     CDS             complement(3919..4332)
                     /gene="hiuH"
                     /locus_tag="ACHOSM_RS21070"
                     /old_locus_tag="pgaptmp_004214"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HGU4560589.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGNYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4465..5136
                     /gene="hprR"
                     /locus_tag="ACHOSM_RS21075"
                     /old_locus_tag="pgaptmp_004215"
     CDS             4465..5136
                     /gene="hprR"
                     /locus_tag="ACHOSM_RS21075"
                     /old_locus_tag="pgaptmp_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HGU4560590.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5136..6494
                     /gene="hprS"
                     /locus_tag="ACHOSM_RS21080"
                     /old_locus_tag="pgaptmp_004216"
     CDS             5136..6494
                     /gene="hprS"
                     /locus_tag="ACHOSM_RS21080"
                     /old_locus_tag="pgaptmp_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HGU4560591.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLHKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(6602..7453)
                     /gene="hchA"
                     /locus_tag="ACHOSM_RS21085"
                     /old_locus_tag="pgaptmp_004217"
     CDS             complement(6602..7453)
                     /gene="hchA"
                     /locus_tag="ACHOSM_RS21085"
                     /old_locus_tag="pgaptmp_004217"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HGU4560592.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMKIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            complement(8045..9208)
                     /gene="ompC"
                     /locus_tag="ACHOSM_RS21090"
                     /old_locus_tag="pgaptmp_004218"
     CDS             complement(8045..9208)
                     /gene="ompC"
                     /locus_tag="ACHOSM_RS21090"
                     /old_locus_tag="pgaptmp_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU4560593.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYLAMMYAETRNMT
                     PYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYVYSKGKDLGGNDYNNNGHQEYVDQDL
                     VNYVEIGATYYFNKNFSTYVDYKINLLDKDDDFYDNNGIATDDVVGVGLVYQF"
BASE COUNT         2675 a         1947 c         2163 g         2731 t
ORIGIN      
        1 tttcttatcg ttcaccattc ttcttttaga cattgatcct atgaaactgg cgcaaagttg
       61 gtggtgggaa ctgaagtggc gtagagaagg ggtcaataat tgagccaaaa aaggaacgcg
      121 gccgtcagat taaagccgcg ggacaaagtc cataatttcc aagagttgtt gaatttccca
      181 gcttttttga aagcctttgt atcatactaa caagtaaaaa aggtttaaat accaaaatta
      241 agctgtattt ttgataagtc ttaccattgt acgatttaca cagtttctgc tcctccttgt
      301 tgatagtgga aagagtgcat tgtgcacttg cgtaaacatt tatgacgcgt tgatgtccat
      361 ggttcacatt tccttgtcag gggatcgact tatttacggc gaggcatcat tcgtaacaga
      421 gtattatctt tctgaatgta gtgatgaaag agtgctgcgc cagcgtgagc tgcgattaaa
      481 aagtaaccta tatttgccca ggtttcgtga atatttttaa tcagtgcttt aatttcgctg
      541 tttggggtaa caaagggaga cacattgaaa ccaaggaaac tccacgattt tccactgtaa
      601 gccatcaaag caatccccag cagaggaagt gcaaggaagg ttatataaag catgatgtgc
      661 attagcgaag ccgctttcat ttgccaggca ggtggcggtg gcacaataga aggatcacta
      721 taacggtgtt ttataatcag gcgtgaaaac attaacaccc aaacgaagat acccgcattg
      781 taatgtgttt ctcgcatcag cagataagta ctactacctt taggaaacca gccacggagt
      841 tccatcgcgg cataggttat tgcaacgaaa agcagcgtta tccaatgtaa ataaatttga
      901 gtttttgaga atcggttcat ttttgtagct cctgtaattt tcacagtcaa gctatcccac
      961 gaaccttaaa aattccttat acagatgtgg ttttaagaat ttgttattta aaaaattgat
     1021 agtgcacatt gtaaccagcc aatataagca atggcgtgag cacaggtatc tttctctgtt
     1081 ggccgtattg tgtatggaat ccgttattgg ttaactcaat gcgggttagc ttattaatat
     1141 acagacacgt catagtgatg agcgtgtctg tagctagctt gcgttcagtg gcgtttgtgg
     1201 atggaccagt ggcagttatg atgacttact aaagcggcat cgaggcgtta tcatgagaat
     1261 actcaatgag acatcatttc ctcgaccact tcttcgctac tcaactgata tggataatac
     1321 gttggccagt tttccatttc atttaataaa gattgctgtg aatcattacc cataaaaatg
     1381 tgaaaatgag atgattttcg tggtgcaatt atatgatcgc taaattgtat atatttaggg
     1441 gctttgcttt caggatcttt acattcgaat aagtaacgaa cgcctttctt gcctgattta
     1501 taggtgagta ttttgtatcc atcgtaatca tatttacagg atgttgtttc attatttcta
     1561 tggaattcaa caatgccgtc ctcaatgcca atcatctcga tatctgttgc ataacctttg
     1621 tgataataat ctttaatttc agcaaatgtt ttagttttat ctgcatccgc tttcttctga
     1681 aagacggggt caagtttgcc actttgcagt aaaggataaa cggattgcca gactccatcc
     1741 cagtcactga gcgttcggtt ttgtacattg gcatcatcaa aaacaccatt agcagctttt
     1801 tgttcgacct ctgttaaggg tttgccgtgt gagtgatgac catgcgaaaa ggcaggagcg
     1861 ctaacaataa agacacctaa agcaacagcc agtttgtgaa gacgaattgc caaaacagtt
     1921 tcctccagag tcattgtata tgatgtgtaa tgttatattg taacatatgg caaaatgcaa
     1981 caactcttag gagatgagag catagttttc tcaggtgtac ttatctatct tattgaaata
     2041 aaatgaatta tgttgatagt ttgaggttga tgacaaacga aacataacct gatgtgatct
     2101 gcgcaggccg gacagggcgc tcgcgccaca tccggcaggg ataacgcgta ctttgtcggc
     2161 aatatgaagt tatacagata atttgttgtg aacctgcttg cgtaaccggt taaaaaggga
     2221 acgcaacttc ttataccgta aggctaaaag cagtacagcc agcccagcgt agatgagcgg
     2281 ctgcggtgaa ataatcttca ctgaccacag ataatgtatt ggggccagga tcgcgacaag
     2341 atagacgaaa ttatgcaact gttgccagtg cttgccgagt tttcgctgca tcgcctgggt
     2401 tgaagtaaac gctaaagcaa gcaaaataac ccagctgata attcctaacg ttaaataagg
     2461 ccgggtaatt aactccttgc ctaataacgc cagattattc acgcccagct ccagcaatgc
     2521 gtaactggtt aaatgcagtg tcgcccaggc aaagcaccat aagcctaaca ggcggcgagt
     2581 gcgtatcaat aacggctgtt ttgcgtagcg tgccagaggt gtgattaaca aggtcgccag
     2641 caaaaatttc agtgcagtgc gaccggtaaa atgctgaata tctttcaccg gatcggcacc
     2701 cagtccaccg tgattgatcg cccagaccag ccagagaaac ggcaacaatc cggcaagatg
     2761 caggctaact ttcagccatg tcacctgttt tgctgtcaga cgcattcaga aattctcccg
     2821 caaatccagg ccacgataca gcgatgccac ctggtcggcg taaccattaa acagtagcgt
     2881 tggctggcgc tgaacatcga ggatgccgcc tgaaccaata aatcgttcgg tagcctgtga
     2941 ccagcgcggg tgatcaacat gcggattaac gttggcgtaa aaaccgtatt cgtcaggcgc
     3001 tgccagattc caggtggttg gcggacgctc gcgggtcagc ttaatactga ctatcgattt
     3061 aatcccttta aagccatatt tccacggcac aatcagtcgt accggcgcgc cattttgtgg
     3121 cggtaacgcc ttgccataaa cacccacggt catcagtgtg agcggatgca ttgcttcgtc
     3181 gagacgcaat ccttcgacat aaggatattt cagcccgccg ccgataaagc ggtcctgctg
     3241 gcctggcatt tgttccggtg cataaattgt ttcgaaagcg acatacttcg cattgctggt
     3301 gggttcggca agcgccagca atttgtgcag cggaaaacca atccacggca ccaccatcga
     3361 ccatgcctcc acgcagcgca tacgataaat acgctcttcc agcgggaagc gacgggttaa
     3421 atcatcgtga tcgagggtca atggttttgc cacttcgccg ctgattttca gtgtccatgg
     3481 atcggttttc aggctaccag cattagcggc gggatcggct ttatccagcc cgaattcata
     3541 gaagttgtta taaccggaga ctttatctac tggcgtcagt ggcagattat tttgccaggc
     3601 ggtaggcttg ctgaactcca gcggttttcc ggcgggggcg ggcgggcgat cgttcccttt
     3661 aaaccagcta agcagatcgg catgcgcagc gtgaggcaaa gaaagtgcag ctgcgctgat
     3721 gcccagtgct tttaacacct gtcgacgctt cataaagaat accgactcgg ccgtaacatc
     3781 tgattctttt aaaaattgat tctttttcat aagccatcac acttttttgt cgttagccag
     3841 caagagccgt ttactgttaa tctgtcaggg agtgtcctcc ctaacagtag cagagatctg
     3901 cttttacata tttatgcatt aactgccacg ataggttgaa tacccatatt ggctaagtaa
     3961 taaaggcaca tgataatgct cgttcacttt attaatatga aattcaacgg ggatctcagg
     4021 gaagaaactt tcaagatttt gtttcttgaa atagtccccg gttttaaata cgacacggta
     4081 attgcccgta gttgcagttt gctcgggcca cagtgcctta attcgtccat ccttatctgt
     4141 tttggcggta ttaagttgta accagccgtt gtccgctttc ttttcaagag tgactgtcac
     4201 gtcggcagcg ggttttccgg tttgctggtt caaaatgtgc acgctaagaa tgttttgttg
     4261 tgcggcataa accaaagaag gtaatgaaaa tgctgccgtt actacggaga gtactaaata
     4321 acgctttaac atgtttatat ccttgtcatg tgaatgagtt ttgaggatag cgaaaaagcg
     4381 gccagggtaa ccttacagca gcattacaat tttgtaatga accaggctgt ttctataaca
     4441 tatgatttat ggcatattat tttcatgaag attttactta ttgaagataa tcaaaggacc
     4501 caggagtggg taacgcaggg gctttccgaa gcgggttatg tcattgatgc cgtttctgat
     4561 ggcagagatg ggctttatct tgcgctgaag gatgattatg cattgatcat tctggatatt
     4621 atgcttccgg gtatggatgg ctggcagatc ttacaaacgt taagaacagc aaagcaaacc
     4681 cctgttattt gccttactgc aagggattct gtcgatgaca gagtcagagg gctggacagt
     4741 ggagcaaatg attatctggt aaaacctttt tcattttctg agttgctggc aagggttcgg
     4801 gcacaattaa ggcaacatca cgctttgaat tcaacattag aaatcagtgg cttaagaatg
     4861 gactctgtaa gtcaaagtgt gagcagggac aatatcagta ttacactgac gcgcaaggag
     4921 tttcagttac tttggctact ggcctccaga gctggcgaaa ttatacccag aacggttatt
     4981 gcgagtgaaa tttggggaat caactttgat agtgatacca atacggtgga tgtcgccatt
     5041 cgcaggctcc gcgcaaaagt tgatgatcct tttcctgaaa agctaatcgc cacaatccgg
     5101 gggatgggct attcattcgt agcggtaaaa aaataatgaa aagattatct ataaccgtcc
     5161 gtttaacctt gctttttata ttgctgctgt ctgttgctgg cgctggaatt gtctggactc
     5221 tctataatgg cctggcaagt gagttgaaat ggcgcgatga tacaacactc attaaccgga
     5281 cagcacagat caagcagttg ttaattgatg gggtaaatcc agatacgtta cctgtatact
     5341 ttaaccggat gatggatgtt agtcaggata tcttgatcat tcatggtgat ggcatcaata
     5401 aaattgttaa ccggacaaat gtcagtgatg acatgttaaa taacatacct gctagtgaga
     5461 caatcagcgc agctggcatt tacagaagca ttattaatga tactgagatt gatgctttac
     5521 gaattaatat tgatgaagtt tcgccatcat taacggttac tgtggctaaa ttggcttcag
     5581 ccagacataa catgcttgaa cagtataaaa ttaatagcat tataatttgc attgtcgcca
     5641 ttgtactttg ctcagtatta agtccgctgt taatcagaac gggattacga gagatcaaaa
     5701 agttgagtgg tgtaacggaa gcgctgaatt ataacgatag ccgggagcct gttgaggtta
     5761 gcgcattacc gagagaacta aaacctcttg ggcaggcgtt gaataaaatg catcatgctt
     5821 tagtcaaaga ttttgagcgt ctaagtcaat ttgctgacga tctcgctcat gaacttagaa
     5881 cgcccattaa tgcattactg ggtcagaatc aggttacgct cagtcaaacc agaagtatcg
     5941 ctgaatatca aaaaacaatt gccggtaaca ttgaagagct ggaaaatatt tcgcggttaa
     6001 cagagaacat actgtttctt gcccgggcag ataaaaacaa tgttttggtg aaactggact
     6061 cgctttctct ccataaggaa gtcgaaaatt tgttggatta tcttgaatac ctttcagacg
     6121 agaaagagat ttgctttaag gtcgagtgca atcagcaaat ttttgcggat aaaattttac
     6181 tgcaacgaat gttatcgaat cttattgtta atgccattag atattcacca gaaaaatcgc
     6241 gtattcatat aaccagtttt cttgatacca acggctatct taatattgat gtcgccagtc
     6301 caggaacgaa aattcatgag cctgaaaaac tcttccgtag attttggcgg ggagataatt
     6361 cgcgtcattc cgtaggtcag ggacttggtc tttctttagt caaagcgatt gccgaattac
     6421 atgggggaag tgctacgtat cactatctca ataagcataa tgtgttccgg attacattac
     6481 cgcaaagaaa ttaacatagg gcttcagtac gccataaatc aaccatgaaa aaacgcagcc
     6541 acgtcgtgag tattaacgtg gctgcgattg attatgcgtt tacattcaaa cgtaacaggg
     6601 attaatccgc ataagctgcc agcatttcct gcgccgccag tttacccaac gcattcgctg
     6661 cgaaaggact gtcgccggtg agaagtttac ggtccttatg tactcgcccg gtgatgtcgt
     6721 cattaatgat tttcatgccc attttcttca gctcttcgcc gaagtaccag gtgagatgac
     6781 ccggcatata gcctatctct ggcgtttgtt tgtctgcggc gtctgggaat gcgcaaatgg
     6841 aataaccatt cagtgggtta tcgccgtggc gaagcgccag aaaagccgcc gggccgtggc
     6901 aaagggagat aacaaaacgg tcatttttga ttgcccactg taaagcagca gccacgtcct
     6961 ggctttctgg tagaccaata agtgcgccat gaccaccagg aacaaagatt gctgcatatt
     7021 cgctatcagc gttgaggctg gcgacaacat ccgcgagttt cttcggattg cggaacaacg
     7081 atttatgctg ctcaaagaat ggcattactt tttcatcttt gtgcggcata gcccagtatt
     7141 caaacttggt catcagaccg gaaatggttg ccacttcgaa ttcgaaacct gcagcatgga
     7201 gatgatacaa cggcagcaac gtttcaatcg gatggttacc ggtcgagaac agttttccgt
     7261 tatcggtcgg caaataacgt tcgtccgcgg cgatcaccag aattttatgt ttaccgcgat
     7321 acggttttgg atagtccacg ccatcaagat cagagacagg actggtatat tggctaagcg
     7381 aatattctga agggaagaat gcattatctt cagcaatatc gacctgcgga tttttacttg
     7441 tttgaacagt catagtgtat tccttattgt tgcttagtta gggtagtcac tatatttgcg
     7501 actgagctgg tcgctatttt taaccaggct ggaaaattga gtcaatatat cgaaagcggt
     7561 agtcggcggg tgtaagtatg taatgatgat gctgcactgg tgttgaatat tattcacgcc
     7621 aaattttcct tattgagcaa cgtcgtcgca agtggcctca atgattttag accattatca
     7681 gaggtacggg gcgacgggga gagatttagt gctgtggaat attgtggtgc tgatgaatat
     7741 ttatatcccg gattggtaga gaaaaagctt atttcatgca ctatttttgc aaatatttct
     7801 atatgggagg cggttttgtt gcagattaat tcataaagaa atgcatttgg gtcttggctg
     7861 gaagagtcgc tggtagatgt tattgtgcca gcaggggcag ggtaaggtgc tgtatcgata
     7921 tgctttggca attgcgcgtg ccgcagaggg agataaaatg ccaataaaaa agcctgagtt
     7981 cgatgaggga cgaactcagg ctagtgttaa gttttacagt aacacgccgc ggcgcgttgg
     8041 ctgattagaa ctggtagact aagcccacac caacaacgtc atctgttgcg atgccattgt
     8101 tgtcatagaa atcgtcatct ttatccaaca agtttatttt atagtcaaca tatgttgaga
     8161 agttcttatt gaaatagtag gtcgctccaa tttcaacata gttgaccaga tcctgatcaa
     8221 catactcttg atgaccattg ttgttatagt cgttaccgcc gaggtctttg ccttttgaat
     8281 aaacatatgc aagagatggg cgcagaccaa aatcgaactg atactgtgct acagcttcaa
     8341 agttctgggt tttatttgcg atacccacat taccatatgg tgtcatattg cgggtttcag
     8401 catacatcat tgcaagataa acattgttgg cgtcgtattt tgcaccaaat gtccatgctt
     8461 cagctgtatc accaccagca aatttgctgt attcattgta gtgatacccg ccaagagcta
     8521 cttgagaatt ggtacggtca gagtttgagt aagcggcacc taagctcaaa ccgaagtcaa
     8581 agtcatatga agtggaaaga ccaaaaccat caccgttctg gaagcgaacg tcttcgtgac
     8641 catttttagt accttcttga tcttctcctg cattgctgcc ttcgttgtta ccttgatact
     8701 gcaatgcaaa gttcagacct tccaccagac cgaagaaatc actattgcgg taagtagcta
     8761 ccccatttgc acggcctgcc atgaagttat cagtccaggt ataggagtcg ccgccgaata
     8821 caggaagcat atctgtccag gcttcaacgt cgtaaatcac accatagtta cgaccatagt
     8881 caaaggagcc catctcgccg aacttcagac ctgcaaaagc aagtttgaca taagtattat
     8941 cagaaccttc agtgtcgcta gcctttgttt tggtcagtgc ttgaccataa ccagttaact
     9001 gatctgtaat ttgggtctca cctttgaagc ctaactcgat gatagtttca tcgccgtcat
     9061 cgccagggtt atcggaaaaa gtatgacgag catcaacttt tccgtacaga tccagtttat
     9121 tgccattttt attataaatt tcagccgcat ttgctgcgcc agcaactaat aacgccggga
     9181 ccagcattgc cagaactttt cttttcatta tgtattccct tgtgattata atcttcatga
     9241 atatatcaat aagtgccgtt atccaaaaaa aagcacattt ggatactatt ctatgaagtt
     9301 cattttattt taaagattgc atgtaacaag tgtatttaaa agatttcaaa atgtttctaa
     9361 tccatttaat atgattcaga attatgaatt ctaaatataa acattcatga aaattataaa
     9421 tgtatatcaa tatgttatga gttgaatgga aaaataggga gtatcaatga ttgttgcgcc
     9481 aaacggcagg cgtgatcacg cgcacattaa tgaagg
//
LOCUS       DAZKHC020000139         9498 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZKHC020000139 DAZKHC020000000
VERSION     DAZKHC020000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.139"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..285
                     /locus_tag="ACHOSM_RS21095"
                     /old_locus_tag="pgaptmp_004219"
     CDS             <1..285
                     /locus_tag="ACHOSM_RS21095"
                     /old_locus_tag="pgaptmp_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HGU4560594.1"
                     /translation="FPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCILVDDSVAG
                     AQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA"
     gene            352..825
                     /locus_tag="ACHOSM_RS21100"
                     /old_locus_tag="pgaptmp_004220"
     CDS             352..825
                     /locus_tag="ACHOSM_RS21100"
                     /old_locus_tag="pgaptmp_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HGU4560595.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            874..1461
                     /gene="cbrC"
                     /locus_tag="ACHOSM_RS21105"
                     /old_locus_tag="pgaptmp_004221"
     CDS             874..1461
                     /gene="cbrC"
                     /locus_tag="ACHOSM_RS21105"
                     /old_locus_tag="pgaptmp_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HGU4560596.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(1523..2245)
                     /locus_tag="ACHOSM_RS21110"
                     /old_locus_tag="pgaptmp_004222"
     CDS             complement(1523..2245)
                     /locus_tag="ACHOSM_RS21110"
                     /old_locus_tag="pgaptmp_004222"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGU4560597.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(2260..3429)
                     /locus_tag="ACHOSM_RS21115"
                     /old_locus_tag="pgaptmp_004223"
     CDS             complement(2260..3429)
                     /locus_tag="ACHOSM_RS21115"
                     /old_locus_tag="pgaptmp_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4560598.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNGSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(3456..5072)
                     /gene="bglH"
                     /locus_tag="ACHOSM_RS21120"
                     /old_locus_tag="pgaptmp_004224"
     CDS             complement(3456..5072)
                     /gene="bglH"
                     /locus_tag="ACHOSM_RS21120"
                     /old_locus_tag="pgaptmp_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HGU4560599.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNKNGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWGGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYADASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETDAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(5141..6551)
                     /gene="bglB"
                     /locus_tag="ACHOSM_RS21125"
                     /old_locus_tag="pgaptmp_004225"
                     /pseudo
     CDS             complement(5141..6551)
                     /gene="bglB"
                     /locus_tag="ACHOSM_RS21125"
                     /old_locus_tag="pgaptmp_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
     gene            complement(6570..8447)
                     /gene="bglF"
                     /locus_tag="ACHOSM_RS21130"
                     /old_locus_tag="pgaptmp_004226"
     CDS             complement(6570..8447)
                     /gene="bglF"
                     /locus_tag="ACHOSM_RS21130"
                     /old_locus_tag="pgaptmp_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HGU4560600.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFMPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTSLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQVAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVRLNGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(8581..9417)
                     /gene="bglG"
                     /locus_tag="ACHOSM_RS21135"
                     /old_locus_tag="pgaptmp_004227"
     CDS             complement(8581..9417)
                     /gene="bglG"
                     /locus_tag="ACHOSM_RS21135"
                     /old_locus_tag="pgaptmp_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HGU4560601.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         2445 a         2378 c         2120 g         2555 t
ORIGIN      
        1 ttcccggata aactgttcag cggctacgat attcagcgct ggaagccaga cccggcgtta
       61 atgttccatg cggcaaaagc gatgaatgta aatgtagaaa actgcattct ggttgatgac
      121 tcagttgccg gtgcgcaatc tggtatcgac gcaggtatgg aagtgttcta cttctgcgcc
      181 gacccgcaca ataagccgat cgttcacccg aaagtcacca cctttaccca tctttcgcag
      241 ttaccggaac tgtggaaagc gcgtggttgg gatattacgg catagttctt cacactccct
      301 tcacttaccc cgcttaaatt ggcgctcaaa ggtaagtaaa gggtgtttga tatgtctgtt
      361 tcacgtcggg taatacatca cggactttat tttgcggttt taggaccgtt aattggtgtt
      421 ctgtttcttg tcctctacat attcttcgca aaagaaccgc tggttctttt ggtaataata
      481 caggtacttc cattgtttct cttattgtcg attactacgg gagctattcc tgcattgtta
      541 accggcgtga tggtcgcctg tctgccggaa aagatcgggt cacagaaaaa ttatcgttgt
      601 ctggctggtg gcataggtgg cgtcgttatc accgagatct attgtgcagt tattgtacat
      661 attaagggca tggcttcctc ggagttgttt gaaaacattc tttctggtga cagtctcgtt
      721 gtccgcatca ttcctgcatt gctggcaggt gtggtgatga gcagaatcat tacccgtctg
      781 cccggattgg atatttcatg tcctgaaaca gactctttaa gttaagcggg atactttatc
      841 tttgggctac tcaaaagcag acaggatgtt actatgactc aaaatatcag gccgttaccc
      901 caattcaaat atcatcccaa gccactggaa acaggcgcat ttgaacagga taaaaccgta
      961 gagtgcgatt gctgtgaaca acagacgtca gtttattact cgggtccctt ttattgcgtt
     1021 gatgaagttg aacatctctg tccgtggtgt attgcggacg gttctgctgc tgaaaagttt
     1081 gcaggtagtt ttcaggatga tgccagcata gaaggtgttg aatttgagta tgatgaagag
     1141 gacgaatttg ccggtattaa gaacacatat cctgatgaaa tgctgaaaga gttggttgaa
     1201 cgcacgccag gttatcatgg atggcaacag gaattctggc ttgcgcattg tggcgatttc
     1261 tgtgctttta tcggctatgt gggctggaat gatataaaag atcgcctcga tgaatttgcc
     1321 aaccttgaag aagattgtga gaatttcggt attagaaatt ctgatctagc taaatgcctg
     1381 caaaagggtg gtgattgtca gggttatctc ttccgctgtc tccactgcgg caagctgaga
     1441 ctgtggggtg atttttcgta gttatttaaa taatgagaac aggccggagc gtaattcata
     1501 catccggcct tatttcttca gcttaaccga gcgctaattc tgcggcagcg gctttatcag
     1561 cgattaccat cagcgaagga tgcagctgca acacggaagc tggaacatct tcggtaaccg
     1621 gaccttgcag cacatttttc agtgcctgcg ctttacctgc gccactgaca attatcagca
     1681 agtttttcgc cgccatgatg cttttcggcc ccatcgtcac gtagctgtca ggcactaacg
     1741 aaaagtcgcc gcccaactca ccatgcgcca caatatcaac catctctccc tggatcggga
     1801 actccacggt ttgctcatgg aaatgggtcg tattcggcaa attgccacaa aaatgaccat
     1861 ctgcacctaa tcccagcacc accaaatcca ggccgccttc acgcgccagt ttctgatcat
     1921 gctcgcggta gttatcaata gtgagcttct ggatattctc ttctttgatc cccgcagggg
     1981 tgaaaaacag attacgcaga ttggtaatcg ttacaccttc tccctctttg ccgcgaaagg
     2041 gaatttcatc gaaattatag aaatagcagt tatcgtacca gggcttacct ttaaccaggg
     2101 tggtgaggta ttcatacatg cctttgggcg tgctaccggc ggtaattgcc aggttaacac
     2161 gacgcgtctt cgacatataa ccaagcagat ggtgtgccgc gacacggctc atttcctggt
     2221 aatcttcggt aatgattaat ttcatcttag tgccttattt tatttaaata atttctgtac
     2281 gaaggctgca taggcaggcc gccagacatc catttcatgg ttcaggccgg gatattcctg
     2341 gtaatcaaac ttaatttttt tctgctcaag ctcagttttc agcccggcga tatccttgcc
     2401 ggttacgaca tctttatcac caacgaccac agtaaaatta cgtagttgct ggttaatagc
     2461 tgccggatcg ttcagccggg ccgcgacacc ttcatccggt acggttgtcg tggtaacacc
     2521 actgaatgtg gccagccagc caaagctttc cagatgattc attccggaaa ccagcgcctg
     2581 gtacccgcct tgtgaaagcc ctgccagcgc gcgaccatcg gcatctttac ggacattaaa
     2641 acgcttgcta atcagcggga taatatcgtt catcagttcg cgatctgccg ctttagcatt
     2701 cagcggataa aagactttac gtctttcctg aggcacgaaa tcttcgggaa taatgccctt
     2761 cgcatcggtt tcggtatccg ggatcaccac cagcatcggt ttaattttcc cttcagccag
     2821 caggttatcc atgatttgcg ggatacgccc ctgatcgata gcggaacgtc cggtatcacc
     2881 aaaaccgtga tagaaataga gcactggcaa aggctcgccc atgccggtgt atcctggcgg
     2941 ggtccagaca tacatctgac gttcagattg caatgcgttg gagtggtaag ttatggcgat
     3001 caaatcacca tgcgcaacag aacgcgtatc cagataactg cctggcacca gaatcatact
     3061 ggagttaacc tggcgctgag gcttagtcat tgcggtgcct gtatcaatgc tgcgtacacc
     3121 atcaacattg aaaaaatact cgtacaggtt gcctttcagg ataggtgtgc gccacgacca
     3181 gactcctgct tcgtctttgg tcatcgggtg aatattgtcc ggaaccggaa cacccaccac
     3241 tacagagcca ttttttgccc caggggcaaa gtagcgaaag gtcacgctgt tatcggcatt
     3301 gacctgagtc acatactgtt tcaccgggat agtgggtgcc gggctggctg gcatatccgc
     3361 tgctatggcc cactgagcag aaataccgct ggcaatagcc agcgttaaag cagcaatttt
     3421 tatattcatc gtaatattcc tttcattaag ttccattacc accagatttc agcctgggca
     3481 ccgacagcaa actggtcgtc tttattgtct tcgaaggtga agccatccag ttcgttttcc
     3541 agggctttga tatacgtggc gtagaaacga atttccggac gcgaggtcaa catactggta
     3601 ttgactttaa aggtatggaa gagcgtggtt ttataaccag actcattaaa tttattacta
     3661 ttcgcatctt tgttttgctg ggtgaaatag cccagttcaa cacctgtctg gttatattgg
     3721 tcccaaatat aggccgggcg aaccaccgca cgaatagatt cgaaatcaga gtgtgcgtct
     3781 gtttcgtagc tataaatatc gttaccgaag gagtaaacaa tcgcgttagc cacaataaaa
     3841 tgatcgccga tataggcttc gccctgcgag gtcagacgaa ctgctgttcc gccggtgtga
     3901 tcaccataat aacgaccatt aaaggtggta aatggacttg cgtcagcata acggccaaag
     3961 ttactggcga tagagttatt cgcaaccagg aaggagaatt cgttgaagcc acctttatca
     4021 aatttctgcg ttaaagatgt gccgaacatc caggtatctt tccagtcata atacccgtta
     4081 ttatcctgat tatctttttc cgatgcgctt tcgtttgccg tgacataacg accactcacc
     4141 attaaggtcg ctttatccca taacgggata tctttatagc gtaaatcaat agtattggta
     4201 ttaatctgct gtttgttttg caggctgcga tcgtaatcat cgacatcttc gcgaaccagc
     4261 gcgatatcaa ttttacccgg accgactttc cagttttcca gacctacacc cgctgcggca
     4321 tcagtacgct gcgttttcca gtccagcatc tggatttcaa ttttcggcgc accgtgttta
     4381 cccccccaga aatcagcctc tggcgcaaag ggcaggaaac ctttggtggt aacgtacata
     4441 tcggagaact gcataaagtt ctcgccaccg gcgttatcgc caaaccagcc ggtagagtac
     4501 tgctgaccaa cgttaccatc catcatcaca acggcatcaa cccgtttgcc gtttttgttg
     4561 tagacacgtt gttttaactg caaatcaaac cagccggagt attcgttacc aaagcggccc
     4621 agagaaccaa tcgcccatga tttaggtgaa ccatgagagg cggtccccca accagaacgg
     4681 taatagccgt tgtaactaaa gccaatttca tctttcacaa acttactgaa atctttcaga
     4741 gtcatggaag aataaatcga gcctgtctca ctggcatttt tctcttcatt tttgaccagt
     4801 acagcatcag gttttgcggc actactggtc gggaacggat tggcggcata cccttgatca
     4861 ttcgtactta cgctacgatt caccgtcgct ggcgtatatt ttttcttctc ttcatcttta
     4921 tactttttga gttcgctttg cgtttctctt aacgcttttt ctaataactc aaggcgttgt
     4981 tccaccgtta atgattcagc caatgattgt gcagaaaagg ctaacggtgc cattaataag
     5041 atggcagagg taataagatt tcgtctaaac ataatatccc tttatggtgc aaagaaagaa
     5101 ttaacgtatc gcatccagac tgttctgaat gcgacgataa ttaaggtgct ttattggtta
     5161 ttttttaatg acagcccccg cgttttgatc acttctgcat accatccgaa gcttttctta
     5221 cgcgttcttg tgaggcttcc ttcgccatta tcatcacgat ccacataaat aaagccgtag
     5281 cgcttagaca tttgtgaatg agatgcactg actaaatcaa ttggccccca actggtgtac
     5341 cccataatat ccacaccatc ggcaatcgct tcatttacct gtaccaggtg atcgtttaaa
     5401 taggcaattc gataatcgtc ctgtatcgaa ccatccgctt caacgctgtc ttttgcgcct
     5461 aatccgttct cgacaataaa taacggtttt tgataacgat cccaaagcgt atttaacaga
     5521 acccgtaatc caaccggatc aatttgcccc ccccactctg aacttttcag atgcggattg
     5581 gggatcatat tcagtatgtt gccctgcgca tttttattaa tgctttcgtc gtgggaaaca
     5641 caaccagtca tgtaataact aaaagagatg aaatctacag tatgttttaa atcttctgcg
     5701 tcactttcag tcatctcaat ggtgatattg tggtcgcgga agaaacgctg catatagccg
     5761 ggatactggc cacgcgcctg aacatcacca aagaacatcc agcgccggtt ctcttccatg
     5821 gcctgcaaca tatcctgtgg ctggcaggtg agggggtaaa ccagcccacc gagaagcata
     5881 ttgccgattt tcgcttcggg gatcaggcta tgacaggctt taactgcccg cgcactggca
     5941 accagttgat gatggatcgc ctgataaact tccgcctcgc cactctcttc tgccagcccc
     6001 acgcccgtga atggcgcgtg taacgacata ttgatttcat taaacgtcag ccataacgct
     6061 actttatgtt ggtagcgggt aaagaccgtg cgggcgtaat gctcgaagtg atcgatgacc
     6121 gcccgattag cccaaccgcc gtagtttttc accagcccat atggcatttc gtaatgggat
     6181 aacgttacca gcggcttgat ccccgcctgc gccatttcat caaacagccg atcgtaaaac
     6241 gctaaccccg cttcattcgg ttcggcttcg tcgccctgag ggaaaattcg cgcccaagca
     6301 atggaaatac gcagacaggt gaagcccatc tcggcaaata acgcgatatc ttccgggtaa
     6361 cggtgataaa aatcgatggc gacatctttg atattctctt tccccaggat gcgcggttcc
     6421 atttttccca ttacgccatg aggctgtaaa tctgaggtcg tgatcccttt gccatcttcc
     6481 agccaggcac cttccacctg attggcagct gttgcgccac cccaaagaaa tgtttctgga
     6541 aatgctttca taattaactc cttttatcgt tagcgaatga tggataacag cggttcacct
     6601 gcgcttatct gcgccgtgcc gtggggtaat acgtctgtaa aatcatcgct attactgatt
     6661 aataccggcg tcgtcagatc aaatccggcc tcgcgaatag cagggatatc aaaagaaatc
     6721 agccgatcgc ctgtattgac cttgtcaccc acgttgacgt gagcggaaaa gaatttgccg
     6781 ttcagtctta cggtgtcgat accgacatga atcaggatct ccacaccatc atctgactca
     6841 atgccaatgg catgtaatgt ggcgaacaac gaagcaattc gacccgcaac cggagaacgc
     6901 acttcaccaa ccgagggcag aatggcaata cctttaccca acaggccgct ggcaaacgtg
     6961 gtatcagcga cgtgaatgag cggcacaatc tctcccgtca tcggtgaaca gataccgccc
     7021 tgctcaggtg gtgtaataac ctctggtgtt ttctcttgcg gggcaacctg cgctggctga
     7081 cgtttactgg tgatgaaatg aagcatcaac gtaccgacaa atgcgcaacc gatggcaatg
     7141 acaccgccaa taacgctggc ccagacggtg aaatcaatcc ccgttgatgg gatggtttgc
     7201 atgaaggtga aaatacttgg caaaccaaag gagtagactt tcgtttgcgc gtagccaatg
     7261 atggtggccc ccaatgctcc actgatacag gcgataacga aggggtactt acgcggcagg
     7321 ttgacgccat ataccgctgg ttcggtgata ccaaacagac tcgtcaacgc cgctgatccc
     7381 gccaccactt ttttctgcgc atcgcgttcg cagaggaaga cgccgagcgc cgccccgacc
     7441 tgcgccataa tggcgggcat taacagcggg atcatggtgt cgtagcccag cacggtgaag
     7501 ttattgatac acagcggcac caggccccag tgcagtccga acatgacgaa gatttgccag
     7561 aagccgccca ttaccgcgcc cgcaaatgca ggaaccgcct gataaagcca gagataaccg
     7621 gcggcaatca gttcacttat ccaggttgat agcggcccca ccagcagaaa ggtgacgggt
     7681 gtgataacca tcagacatag caatggtgtg aagaaatttt tgattgccga cggtaaccac
     7741 gcattaagtc ggcgttccag aatgctgcac aaccaggcag aaaaaataat gggaataacc
     7801 gatgacgagt aattcaacaa tgtgaccgga atacccagga aatccagccc cagcgcatcc
     7861 gctttttgcc cgttctcgaa agcagtcaga attaatggat gcactaacgc tccaccaatc
     7921 accatggcag taaatggatt accgccgaag cgtttccccg cggtgtatcc caggattatc
     7981 gggaagaacc aaaacaaggc atcactggcg ctgaataaaa ttaaataagt accactttgt
     8041 tcggtcgtcc actgaaaagt gagcgccaga gccagcatac ctttcaggat cccggttgcc
     8101 gccatcagac cgatcagagg cataaaaata cctgaaataa cataaacaaa gcggtttagc
     8161 agattacctt tctcatcatt ttccggtgcc tgttgcgctt tttcgccaag gcctgccacg
     8221 ctgttaaccg ccaggaagac atcggccaca tggttaccta tgaccacctg aaactgacca
     8281 ccgctttcca ccaccataat aataccgggg gtctttttca gtacctcagc ttgcgctttg
     8341 ctttcatcct ttaacttaaa acgtaatcgc gttgcgcaat gcatcagact cacaatgtta
     8401 tcagcgcccc cgactcctgc gactattttt ctggctaact ccgtcataac ttgccctcta
     8461 ccgctttgcg gcaaaactcc aaaaaaaaac ctgaaaaaaa cggcctgacg tgaatcaagc
     8521 aatttttttc aggttttgcc cgcttagtgc ggtaacaatc ctttactcag taataatatt
     8581 tcagtgttct ttgcgcacgc gctctatatt tatggctaaa aacataatct ctgcgggtga
     8641 aattttacgt tgatactgca aaccaataaa aatggcgatc cgttccgcac attgccatgc
     8701 ttgcgggtaa ttttgtttta ctgcttgttg taatgattca tcactatcgt taattgaagc
     8761 atgttcaaga atacgccagg ataaaaactt cagatgtgta accagtcgct gataactcaa
     8821 gctttcttcc tggtaattaa ggctgaactg aaattttatt aattgcagca tttcgcgcat
     8881 taactgcgtg acacctgcaa catcctccat atttccgctc atttgggcac tgaccagatg
     8941 catggcaata aagcccactt catctttcgg taactgcacg cccaaccgtt tatcaataat
     9001 ggttaacgct tcttccccta gctggaactc tttcgggtaa agccgctgga tatcccacag
     9061 caacgggttg ggcagcaaca cgttttgctg aaagcgttta atcgcaaact ggcaatggtc
     9121 agttagcgag atataaatac tgtcctgtaa ttttcccaag cgctcctgcg ctaaagagat
     9181 aatacgatca caggttgcca tcacctcaag aggaatatga cttaagagtt cgcttaatcg
     9241 cccgttcagt tcatgactgc tcaaggcata ctctttttct attccacttg agttaattct
     9301 ttcgccagcg cgtttttgaa agccaattcc gcgccccatg acgacttttt cccgctgttg
     9361 atcatcaata accaccacaa cattattgtt gagaattttg gtgatttgca tgttcatagc
     9421 aaggaccttt tttataaaca aaaaaaaccg acttcaccag tattctctgg ttatgtcagg
     9481 ttttgcctgc gaatgcag
//
LOCUS       DAZKHC020000140         9468 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZKHC020000140 DAZKHC020000000
VERSION     DAZKHC020000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.140"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            156..665
                     /locus_tag="ACHOSM_RS21140"
                     /old_locus_tag="pgaptmp_004228"
     CDS             156..665
                     /locus_tag="ACHOSM_RS21140"
                     /old_locus_tag="pgaptmp_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4560602.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            637..2565
                     /locus_tag="ACHOSM_RS21145"
                     /old_locus_tag="pgaptmp_004229"
     CDS             637..2565
                     /locus_tag="ACHOSM_RS21145"
                     /old_locus_tag="pgaptmp_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU4560603.1"
                     /translation="MNISEQQLNNMMAAVSVALQPLVRVVPMTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNCMGNDQVRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPSPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            2549..2755
                     /locus_tag="ACHOSM_RS21150"
                     /old_locus_tag="pgaptmp_004230"
     CDS             2549..2755
                     /locus_tag="ACHOSM_RS21150"
                     /old_locus_tag="pgaptmp_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HGU4560604.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRHPLGVRL"
     gene            2752..4344
                     /locus_tag="ACHOSM_RS21155"
                     /old_locus_tag="pgaptmp_004231"
     CDS             2752..4344
                     /locus_tag="ACHOSM_RS21155"
                     /old_locus_tag="pgaptmp_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4560605.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            4334..5482
                     /locus_tag="ACHOSM_RS21160"
                     /old_locus_tag="pgaptmp_004232"
     CDS             4334..5482
                     /locus_tag="ACHOSM_RS21160"
                     /old_locus_tag="pgaptmp_004232"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4560606.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQAAMPEMTV
                     EKARPILAASPQADAGPSLRDQIMALDEAKGAEARAEQLAACPGMTVESARAVLAAGS
                     GKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5519..5866
                     /locus_tag="ACHOSM_RS21165"
                     /old_locus_tag="pgaptmp_004233"
     CDS             5519..5866
                     /locus_tag="ACHOSM_RS21165"
                     /old_locus_tag="pgaptmp_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU4560607.1"
                     /translation="MVTKNITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            5924..6952
                     /locus_tag="ACHOSM_RS21170"
                     /old_locus_tag="pgaptmp_004234"
     CDS             5924..6952
                     /locus_tag="ACHOSM_RS21170"
                     /old_locus_tag="pgaptmp_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4560608.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWCLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTAADGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7004..7387
                     /gene="gpFI"
                     /locus_tag="ACHOSM_RS21175"
                     /old_locus_tag="pgaptmp_004235"
     CDS             7004..7387
                     /gene="gpFI"
                     /locus_tag="ACHOSM_RS21175"
                     /old_locus_tag="pgaptmp_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4560609.1"
                     /translation="MATKEENQKRLRQLAGLLGREADMSGSAADIAQCVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
     gene            7380..7733
                     /locus_tag="ACHOSM_RS21180"
                     /old_locus_tag="pgaptmp_004236"
     CDS             7380..7733
                     /locus_tag="ACHOSM_RS21180"
                     /old_locus_tag="pgaptmp_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU4560610.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSCPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            7748..8281
                     /locus_tag="ACHOSM_RS21185"
                     /old_locus_tag="pgaptmp_004237"
     CDS             7748..8281
                     /locus_tag="ACHOSM_RS21185"
                     /old_locus_tag="pgaptmp_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4560611.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMPGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVVGKNRYPIDVVKIPMAAPLKQAFDENVDRIRSERLP
                     GELAYALKQQLRIAIKR"
     gene            8278..8673
                     /locus_tag="ACHOSM_RS21190"
                     /old_locus_tag="pgaptmp_004238"
     CDS             8278..8673
                     /locus_tag="ACHOSM_RS21190"
                     /old_locus_tag="pgaptmp_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4560612.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMSAIPALAGMITTM
                     VQQGYDYRRDDDMALWSSADLTYSITYEM"
     gene            8681..9433
                     /locus_tag="ACHOSM_RS21195"
                     /old_locus_tag="pgaptmp_004239"
     CDS             8681..9433
                     /locus_tag="ACHOSM_RS21195"
                     /old_locus_tag="pgaptmp_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4560613.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYTGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWISSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     ATAIKVTPTSGTVEKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMVITMTG
                     VKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
BASE COUNT         2212 a         2227 c         2941 g         2088 t
ORIGIN      
        1 tccgggatcc atgtccggtt tctcttcaag ttaactatat gaaaaatata aaaacaggtc
       61 ttctgtgaac cggacatgaa caaaaaacag acatgtaaac cggacatgac cggttttgtt
      121 gtgattgtga ggtgagagtt tttgcgaggt gaggagtggc tacgcagact gaagttgcca
      181 ggcatttaag tctgaccgat cgccagcttc gcagattgca gaaattgccg ggtgccccga
      241 tatcgaataa gcgagggcaa ctggatctgg atgcctggcg cgatttttac atatcatatc
      301 tgaggagaag taaaaacgat gtgcctgatg gcgatagcga agacgactat gaggagaaat
      361 tgcttattgc cagatgggaa ctgacagcag aacaggctgt tacacagcag ttaaaaaatg
      421 aggtgtcaaa aggaaaactt attgacaccg ggttctgtat ttttgccctc agtaagctgg
      481 caatggcgtt atccagtacg cttgattcca tccctttatc catgcagcga cagtttcctg
      541 atttaacacc gcgccatctt gaccatctga aaacccttat tgcgaagggg gcaaatcagt
      601 gtgcgcgggc aggggataaa ttaccggatt tactcgatga atatatcaga gcaacaactg
      661 aataatatga tggctgccgt ttcggttgcg ctgcagcctc tggtcagggt tgtaccaatg
      721 acggcagttg aatgggctga tcaaaattat tatctgccta aagaatcctc atacggtgag
      781 ggagaatgga aaacgctgcc attccagatc gccattatga actgtatggg taacgaccag
      841 gttcgcacgg ttaacctgat taaatctgcc cgtgttggct atacaaagat gttgctgggg
      901 gtggtcgggt attttattga gcataaatcc cgaaacagtc tgctttttca gcccacggat
      961 tctgccgctg aagattttat gaagtctcac gtggaggcga cgattcggaa cgtgccatgc
     1021 ctgaaagacc tttccccatg gctgggtcgt aaacatcgtg acaatactct cacgctgaaa
     1081 cgcttttcat cgggcgtcgg tttctggtgc ctgggcggcg ctgccgccaa aaactaccgt
     1141 gaaaaatccg tggacgtggt ctgctatgac gaactttcct cgttcgagcc ggatgtcgaa
     1201 aaagagggct cgccaaccct gctgggggat aagcgtattg aggggtcggt gtggccaaaa
     1261 tccattcgcg gctcgacgcc taaaatcaaa ggcacctgcc agatcgaaaa agccgctaac
     1321 gagtcggcgc attttatgcg tttttatgtg ccctgcccgc actgtgggga ggagcagtat
     1381 ctgaaatttg gcgatgagtc cacgcctttt gggcttaaat gggagaagga cagccctgaa
     1441 agtgttttct acctctgtga acatcatggc tgcgtgatcc atcagtctga actggaccag
     1501 agcaacgggc ggtggatctg tgaaaacacg ggcatgtgga cccgtgacgg tctgacgttt
     1561 ttcagcgccc ggggtgatga aattccgccg ccgcgctcca tcacgttcca tatctggacg
     1621 gcgtacagtc cgttcaccac ctgggtacag attgtctatg actggctgga tgcactgaaa
     1681 gatcccaacg gcctgaaaac ctttgtgaac accacgctgg gcgagacctg ggaagaggcc
     1741 gtgggcgaaa aactcgatca ccaggtactg atggataagg tggtgcgtta cacggcggcg
     1801 gtgcctgccc gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag
     1861 atgtatgtct ggggatgggc tccgggagag gaagcctttc tggtggataa aatcatcatt
     1921 atggggcgtc ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caacaaaaaa
     1981 taccgccatg cggatggcac cgaaatgact atttcccgtg tctgctggga caccgggggg
     2041 atcgatggtg aaattgttta tcagagatca aaaaaacacg gtgttttccg ggtgctgccg
     2101 gtaaaaggcg catctgtcta tggcaagccg gtgatcacca tgccaaaaac ccgcaatcag
     2161 cggggcgtgt atctgtgtga agtgggaacg gacaccgcaa aagaaattct ctatgcccgt
     2221 atgaaagccg atccctcgcc tgcggatgaa gccacgtcgt atgccatccg ttttcctgat
     2281 gatccggaga ttttttcgca gacagaggcg cagcaactgg tggcggaaga gctggtggag
     2341 aagtgggaaa aaggaaagat gcgtctgctg tgggataaca aaaagcggcg taacgaagcg
     2401 ctggactgcc tggtgtatgc ctacgcggca ttacgtgtgt ccgtgcaacg ctggcagctt
     2461 gatctggctg tactggcaaa atcccgggaa gaagagacga cccggccaac cctgaaagaa
     2521 ctggcagcga agctgtccgg aggagtgaat ggttacagtc gctgaactgc aggcgctgcg
     2581 tcaggcgcgc cttgatttat taaccggtaa acgggtggtg tctgtccaga aagatggtcg
     2641 cagaattgaa tatacggcgg cttctctgga tgagcttaac cgggcgatca atgatgcgga
     2701 gtcggtactg gggacaaccc ggcgtcgccg tcatccgctg ggagtgaggt tatgaaacga
     2761 acgcctgtcc tgattgatgt gaacggcgtt ccgcttcgtg agagtctcag ctacaacggg
     2821 ggcggtgcag gatttggcgg gcaaatggcg gagtggttgc caccggcgca gagtgccgat
     2881 gcggccctgc tgcccgcgtt gcgtctgggg aatgcccggg cagatgatct ggtgcgcaat
     2941 aacggaatag cggccaatgc ggtggcactg cataaggatc acattgtcgg gcatatgttt
     3001 cttatcagct accgtccgaa ctggcgctgg ctggggatgc gggagaccgc agcaaaaagc
     3061 tttgtcgatg aggtggaggc ggcctggtcg gaatacgccg aagggatgtc tggcgagatc
     3121 gacgtggaag gaaaacgcac gttcacggaa tttatccgtg aaggtgtggg cgttcatgcg
     3181 tttaacggcg aaatctttgt gcagccggtc tgggatacgg aaaccacgca gttattccgt
     3241 acgcgtttta aagccgtgag tccgaaacgg gtggacacgc caggacacgg tatggggaac
     3301 cgttttctgc gggccggggt ggaggtcgat cgatatggcc gtgccgttgc gtaccatatc
     3361 tgtgaggatg attttcctcg ctccgggagt ggacgatggg aacggatccc gcgtgaactt
     3421 cccaccgggc gtccggccat gctgcatatt ttcgagccgg tggaggacgg gcagacccgt
     3481 ggggccaacc agttttacag cgtcatggaa cggctgaaga tgctcgattc cctgcaggca
     3541 acacagcttc agtcggccat tgtgaaagcc atgtatgcag cgacgattga aagtgacctt
     3601 gataccgaaa aggcctttga atatatcgcc ggtgcgccgc aggggcagaa ggataatccg
     3661 cttattaata ttctggagaa gttctccagc tggtatgaca cgaataacgt gacgctgggt
     3721 ggtgtcaaaa ttccgcacct tttccccggg gatgatctga aactacagac tgcgcaggat
     3781 tcagacaatg gattttcggc gcttgaacag gcgctgctgc ggtatatcgc cgccggtctt
     3841 ggcgtttcct acgaacagtt gtcccgtgat tactcgaagg tcagttattc aagtgccagg
     3901 gcctctgcca atgagtcgtg gcgctatttt atggggcggc gaaaatttat tgcggcccgg
     3961 ctggccacgc agatgttttc ctgctggctg gaagaggcac ttcttcgggg gattatccgt
     4021 ccgccacggg cgcgttttga tttttatcag gcgcgatcag cctggtcacg ggcagagtgg
     4081 attggtgccg gaagaatggc cattgacggg ctcaaggagg ttcaggaatc ggtgatgcgc
     4141 attgaggccg gactgagcac gtatgagaaa gagctggcgc tgatgggcga ggattatcag
     4201 gacattttcc gccagcaggt cagggaatct gcagagcggc aaaaagccgg actctcacgt
     4261 ccggtgtgga tagcgcaggc gtatcagcag cagatagcgg agagtcgcag gccggaagag
     4321 gagacaacac cccgtgagac gtaatctttc acacattatt gccgcagcat tcaatgaacc
     4381 gctgcttctg gagcccgcct atgcgcgggt tttcttttgc gcgctcgggc gcgagatggg
     4441 ggcagcaagt ctttcggtac cacagcagca ggtacagctt gatgctcccg gaatgctggc
     4501 tgaaacggac gagtacatgg ccggaggtaa acgaccggcc cgtgtttacc gggtggtgaa
     4561 cggtattgct gtactgccgg tgaccggcac gctggtgcac cggctggggg gtatgcggcc
     4621 attttccgga atgacaggct atgacggcat tgtcgcctgt cttcagcagg caatggcgga
     4681 tagccaggtg cggggcgtac tgctggacat tgacagtccg ggcgggcagg ccgccggcgc
     4741 gtttgactgc gctgacatga tttaccgcct ccgtcagcag aagccggtct gggcactgtg
     4801 caatgacacg gcctgttctg cagccatgct gctggcgtcg gcctgctccc gacggctggt
     4861 tacccagaca tcccgtatcg gctccattgg cgtgatgatg agccatgtca gctatgccgg
     4921 tcatctggcg caggccggtg tggatatcac gctgatttac tcaggggcgc acaaggtgga
     4981 tggcaatcag tttgaagcct taccggcaga ggttcgccag gacatgcagc agcgcattga
     5041 tgcggcgcgc cggatgtttg ccgaaaaagt ggccatgttt accggtctgt ctgttgatgc
     5101 cgtcacggga acagaggccg ccgtttttga aggtcaggcg gcgatgccgg agatgacggt
     5161 ggaaaaagcc cgcccgatcc tggctgcttc accgcaggcg gatgccggac cctcactccg
     5221 tgatcagatc atggcactgg atgaggcaaa aggggctgag gcgcgggctg aacagctggc
     5281 tgcctgcccg ggaatgactg tggagagcgc ccgggctgtg ctggctgcgg gatcaggtaa
     5341 ggcagaaccg gtctctgcat ccacaaccgc cctgtttgaa cgcatcatgg cgaaccattc
     5401 accggcagcg gtacagggtg gcgtgccaca gacgtcagca gacggtgatg cggacgtgaa
     5461 aatgctcatg gctatgccat gaagtcagtg ctgaccatca acaggaggtt tttacaatat
     5521 ggtaacgaaa aacatcactg aacagcgtgc ggaagtacgt atttttgccg gtaatgatcc
     5581 ggctcatacc gccacaggca gcagcgggat ttcctcggca acaccggcac tgacgcccct
     5641 gatgctggat gaagctaccg ggaaactggt ggtctgggac ggacagaaag ccggtagtgc
     5701 agttggcata ctggtactgc cgcttgaagg cacagagacg gtactgacct attacaagtc
     5761 ggggaccttt gcgacggagg caatccgctg gcctgaaagt gtggatgaac acaaaaaggc
     5821 aaatgccttt gccggcagtg ccctgagtca cgcggcgctg ccgtaacacg ttatcaggcc
     5881 accgcggtgg cctgactgat ttctgaatga aaggaactga tttatgggat tgtttacgac
     5941 ccgccagtta ctcggttata ccgaacaaaa agtgaaattt cgtgcgctgt ttctggagct
     6001 gtttttccgc cgtacggtga atttccatac cgaagaggtg atgctggaca aaattaccgg
     6061 aaaaacgccg gtggcggcct atgtctcccc ggttgttgaa ggaaaagtgc tgcgtcatcg
     6121 cggtggtgaa acccgcgtgt tacgtccggg ctacgtcaag ccgaaacacg aatttaatta
     6181 ccagcaggcg gttgagcgtc ttcccggtga agatccggct caactgaacg acccggccta
     6241 ccgtcgtctg cgtatcatca ccgataacct caaacaggaa gagcatgcca ttgtccaggt
     6301 ggaagaaatg caggcggtga atgccgtgct gtatggcaaa tacaccatgg aaggggatca
     6361 gtttgatact gtcgaggtgg attttggacg ctctgaagga aataacattg agcaggccga
     6421 cggtaaaaaa tggtctgagc aggaccgtga tacgtttgat ccgacgcatg atattgacct
     6481 ctactgcgat caggccagcg gtcttgtgaa tattgctatt atggacggta ctgtctggtg
     6541 tctgctgaat ggttttaagc tgttccgcga aaaactggat acccgtcgcg gctcaaattc
     6601 acaactcgaa acggcagtga aagacctggg ggcggtggtg tctttcaaag ggtattacgg
     6661 cgatctggcc attgtggtgg cgaaaacgtc ttatgtggca gaggacggta ccgaaaaacg
     6721 ttatctgccg gagggcacgc tggtcctggg aaatacggct gcagatggga tccgttgtta
     6781 cggtgccatt caggatgcgc aggcgttgtc cgaaggtgtg gtggcttctt cccgttaccc
     6841 gaaacactgg ctgaccgtgg gcgatccggc ccgtgaattt accatgacgc agtccgcacc
     6901 gctgatggtg ctgccggatc cggatgagtt tgtggtggtg caggtgaaat aatccgtgag
     6961 cgggggcgaa atgcccccgt gtcttttttc acagggggct gatatggcaa caaaagaaga
     7021 aaatcagaaa cgtcttcgtc aactggctgg cctgctgggg cgcgaggcgg atatgtcggg
     7081 gagtgctgcg gatattgcgc aatgtgtgtc tgagtgggaa gaggagcttg ctgcttcccg
     7141 ggagggcatt atgcctggtg atgagagcgg gcctgagcaa aatcacacag acgatggtga
     7201 gcagttgcac aacactgatg ctacggatga tgttaaagcg gtccgtgtgc ggaaatgcct
     7261 gcatgtgatg gggtattgcc cggagacagg ccgtcccgtt gaactgacgt accggggcat
     7321 gcgtgttctg gtgccatcac cactggcgac agccatgata cagcacggaa cggctgagca
     7381 tgcgtgattt tcagaatgcc tttgatgctg cccttgccgg ggtggacagc acgattgttg
     7441 aagtgatggg gctctgtgcg cagttcacct cgggggcaca gtgtggcagc gaagttcagg
     7501 gggtttttga cgatccggag tcgctggggt ttgccggtag cggggtccgt attgaaggaa
     7561 gctgcccgtc attatttgta cggacggata cggttcgtgc tgtgcggcgt ggtgacacgc
     7621 tgaccattaa cggcgagatg ttctgggtgg accgtgtttc tccggatgat ggagggagtt
     7681 gttatctctg gctcaaccgt gggcaaccac cggcagttaa ccggcgacga taaacgcagg
     7741 gtgaattatg gcgataaaag ggcttgatca ggcgattgaa aatctgagcc gggttcgtaa
     7801 aaacgccatt ccggcggctt cagcaatggc cattaaccgc gtggccacaa cggcgattaa
     7861 tcagtcttcg tcacaggttg cccgggagac cagggtgagc cggaaactgg taaaggaacg
     7921 gtccagactg aaacgggcca cggtcagaaa tccgaatgcc agaattatcg ttaaccgcgg
     7981 tgatctcccg gtgattaagc tggggatcag gatgccgggg cgtcgtccgg acagcatact
     8041 caaagccggt cagcatcgtt atcagcgggc atttattcag cgattaaaaa atggtcgctg
     8101 gcatgtcatg cagcgtgtgg tcgggaaaaa ccgttacccc attgatgtgg tgaaaatccc
     8161 gatggcggcc ccactgaaac aggcgtttga tgagaatgtt gaccgtatcc ggagtgaacg
     8221 tctgcccgga gaactggcat acgcgctgaa acaacaactg aggattgcga taaaacgatg
     8281 aaacacactg atatccgtgc cgcagtgctg gatgcactcg agcagcatga acacggggcg
     8341 acgctgtttg atggtcgccc cgttgttttt gacgaagagg attttccggc cgtcgcggtt
     8401 tatctgacgg atgcagagta taccggtgaa gagctggatg cagatacctg gcgggccaca
     8461 ctgcatattg aggtgttttt accagcacag gtaccggatt cagagctgga ttcgtggatg
     8521 gagtcccgga tttatccggc gatgtccgcg atccctgcac tggcagggat gattaccacg
     8581 atggttcagc agggctatga ctatcgtcgt gatgacgata tggcgttatg gagctctgca
     8641 gatctgactt attcaattac atacgagatg tgaggacgat atgccaacac caaatcccct
     8701 ggcgccggta aaaggtgccg gtaccactct gtgggtttac accggcaagg gtgatgctta
     8761 tgcaaacccg ttgtcagatg atgactggca gcgactggct aaggtgaagg atctgacgcc
     8821 gggcgagatg acggcagaat cctacgatga taactacctg gatgatgaag acgcggactg
     8881 gagcgcgacc gggcagggac agaaatctgc aggtgatacc agttttacac tggcctggaa
     8941 accgggagaa gaaggtcaga aagggcttat aggctggttt gaaagcgggg atgtgcgggc
     9001 ctataaaatc cgttttccga atggcacggt ggatgtgttt cgtggctgga tcagcagtat
     9061 cggtaaggca gtgacggcga aagaagtgat cacccgaaca gtgaaagtga ccaacgtggg
     9121 caaaccttcc gtggcggaag aacgcagcga aattacgccg gccactgcaa ttaaggtgac
     9181 accgacatcc ggtaccgtgg aaaaaggaaa aacaaccacc ctgactgttt cttttgagcc
     9241 ggaaagtgca accgacaaga cgttcagagc ggtttccgcc gatccgtcaa cgggaaccat
     9301 tgctgtgaaa gatatggtga tcactatgac gggggttaag gctggaaaag tgagtatccc
     9361 cgtgatttcc ggtaatggtc agtttgccac ggtagctgaa gtcaccgtta ctgaagcggg
     9421 cgctgcaggg taaacggagg tcatacatgt ttctgaaaac agaacaat
//
LOCUS       DAZKHC020000141         9414 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZKHC020000141 DAZKHC020000000
VERSION     DAZKHC020000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.141"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(37..821)
                     /locus_tag="ACHOSM_RS21200"
                     /old_locus_tag="pgaptmp_004240"
                     /pseudo
     CDS             complement(37..821)
                     /locus_tag="ACHOSM_RS21200"
                     /old_locus_tag="pgaptmp_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1151..1227)
                     /locus_tag="ACHOSM_RS21205"
                     /old_locus_tag="pgaptmp_004241"
     tRNA            complement(1151..1227)
                     /locus_tag="ACHOSM_RS21205"
                     /old_locus_tag="pgaptmp_004241"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1191..1193),aa:Asp,seq:gtc)
     gene            complement(1360..2091)
                     /gene="dnaQ"
                     /locus_tag="ACHOSM_RS21210"
                     /old_locus_tag="pgaptmp_004242"
     CDS             complement(1360..2091)
                     /gene="dnaQ"
                     /locus_tag="ACHOSM_RS21210"
                     /old_locus_tag="pgaptmp_004242"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HGU4560614.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            2156..2623
                     /gene="rnhA"
                     /locus_tag="ACHOSM_RS21215"
                     /old_locus_tag="pgaptmp_004243"
     CDS             2156..2623
                     /gene="rnhA"
                     /locus_tag="ACHOSM_RS21215"
                     /old_locus_tag="pgaptmp_004243"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HGU4560615.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(2620..3342)
                     /locus_tag="ACHOSM_RS21220"
                     /old_locus_tag="pgaptmp_004244"
     CDS             complement(2620..3342)
                     /locus_tag="ACHOSM_RS21220"
                     /old_locus_tag="pgaptmp_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4560616.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            3376..4131
                     /gene="gloB"
                     /locus_tag="ACHOSM_RS21225"
                     /old_locus_tag="pgaptmp_004245"
     CDS             3376..4131
                     /gene="gloB"
                     /locus_tag="ACHOSM_RS21225"
                     /old_locus_tag="pgaptmp_004245"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HGU4560617.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKLPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            4203..5561
                     /gene="mltD"
                     /locus_tag="ACHOSM_RS21230"
                     /old_locus_tag="pgaptmp_004246"
     CDS             4203..5561
                     /gene="mltD"
                     /locus_tag="ACHOSM_RS21230"
                     /old_locus_tag="pgaptmp_004246"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HGU4560618.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPYATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKDSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(5610..6233)
                     /locus_tag="ACHOSM_RS21235"
                     /old_locus_tag="pgaptmp_004247"
     CDS             complement(5610..6233)
                     /locus_tag="ACHOSM_RS21235"
                     /old_locus_tag="pgaptmp_004247"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4560619.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(6237..7037)
                     /locus_tag="ACHOSM_RS21240"
                     /old_locus_tag="pgaptmp_004248"
     CDS             complement(6237..7037)
                     /locus_tag="ACHOSM_RS21240"
                     /old_locus_tag="pgaptmp_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU4560620.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            7278..8192
                     /gene="yafC"
                     /locus_tag="ACHOSM_RS21245"
                     /old_locus_tag="pgaptmp_004249"
     CDS             7278..8192
                     /gene="yafC"
                     /locus_tag="ACHOSM_RS21245"
                     /old_locus_tag="pgaptmp_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HGU4560621.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAVQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(8189..8992)
                     /gene="dkgB"
                     /locus_tag="ACHOSM_RS21250"
                     /old_locus_tag="pgaptmp_004250"
     CDS             complement(8189..8992)
                     /gene="dkgB"
                     /locus_tag="ACHOSM_RS21250"
                     /old_locus_tag="pgaptmp_004250"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HGU4560622.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(9155..9231)
                     /locus_tag="ACHOSM_RS21255"
                     /old_locus_tag="pgaptmp_004251"
     tRNA            complement(9155..9231)
                     /locus_tag="ACHOSM_RS21255"
                     /old_locus_tag="pgaptmp_004251"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9195..9197),aa:Asp,seq:gtc)
BASE COUNT         2529 a         2233 c         2213 g         2439 t
ORIGIN      
        1 ttgccgccag aaattcctgg cggcagatat aatttattat tgatagcggt taaccaaatc
       61 ttttaccatc gctttatagg tcttttgctt aagttgcatg atttgctgct ctgtcatatc
      121 agtcgtactg gcttcattct tgtcgggcaa tggtggaagc actttatatt cataattcac
      181 cggcgaccag gtagcccact cacctgttgc cacatccacc agcgtgaatc gtaccagata
      241 gcgcagtgac atcgtgtcag tcaatttttg atttttataa tctgaccaaa ctattgattt
      301 caatccagag tcatattttc ctgtctgcaa cgtatcctgg aaaacaataa tcgctttctg
      361 atgccctttt gcagccacaa acgcagtgcc tgcatccagt tcatattctc agcactggca
      421 acatcctcgt tctcattttt atctttgttt ttgttacaag tcagaggctt ttgcctgtcc
      481 gggataccag agaatgtgga aacagtataa tattttcgca tctcctcctg cataatggtt
      541 tccggtgcgc ggttgccgga ttgaacaagt attacaggag aattaagggg aacgcggaac
      601 tcatctccat caagcgcagc ttgaatatcc tcttcggata cagccagagt ggtttcatta
      661 ccgaataaat ctttgtctgt cagttgctgg ggagtgtttt tattaatatt actttgtgtg
      721 acttgagctt ttttcttctt ttcatcaata gaagattcag aggcacatcc tgcaagcagc
      781 gagaataaca cacatatgca acaaagcttt tttgaattca taattggaca ctccctcgcc
      841 tgtcataatc atggtaatgt ttagctaacg atttttaatt atatactcca cttgaatcag
      901 aacaaacaaa aataacagta tatataaatt cattaaatca atagaaaaat gaaaatcata
      961 acattcaaat atatttaaat tgaatgattc gatagtatga ttaaatatta atgatatggt
     1021 ctaccatatg tgattaacac atttacagca taacaaaacc atagaccgaa gaaatagcgg
     1081 gatttaaatg caaaaaaaac gcaaaattaa aaatcttgcg gttctctgaa ctcattttca
     1141 tgagtgaatt tggcggaacg gacgggactc gaacccgcga ccccctgcgt gacaggcagg
     1201 tattctaacc gactgaacta ccgctccgcg ttgtgttccg ttgggaacga ggcgaatatt
     1261 acggattgcc tcacccttcg tcaacggttt ttctcatctt ttgaatcgtt tgctgcaaaa
     1321 atcgcccaag ccgctatttt tagcgccttt tacaggtatt tatgcccgcc agaggcagct
     1381 tccgcccttc ttctccacca gatcaagacg ggcttcatga gctgcaagct cttcatctgt
     1441 cgcaaaaaca acgcgtaact tacttgcctg acgtacaatg cgctgaattg ttgcttcacc
     1501 ttgttgctgc tgtgtctctc cttccatcgc aaaagccatc gacgtttgac caccggtcat
     1561 cgccagataa acttccgcaa ggatctgtgc atcgagtaat gccccgtgta gcgttcgttt
     1621 actgttatct atttcgtagc gggcacataa cgcatcgagg ctgttgcgct taccaggaaa
     1681 cattttcctc gccaccgcaa ggctatcggt gaccttacag aaggtattgg tcttcggaat
     1741 atcgcgctta agcaacgaaa actcgtagtc cataaagccg atatcgaacg ctgcgttatg
     1801 gatcaccaac tccgcgccgc ggatatagtc catgaactca tcggctactt cggcaaacgt
     1861 gggcttatcg agcaaaaatt catcggcaat accatgtacg ccaaaggctt ccggatccac
     1921 cagccgatcg ggtttgagat aaacatggaa gttattgccc gtcaggcgac ggttcaccac
     1981 ttcaacggca ccaatctcaa tgatcttgtg gccttcatag tgcgcaccaa tctggttcat
     2041 accggtggtt tcggtatcga gaacgatctg gcgtgtaatt gcagtgctca tagcggtcat
     2101 ttatgtcaga cttatcgttt tacagttcga ttcaattaca ggaagtctac cagagatgct
     2161 taaacaggta gaaattttca ccgatggttc gtgtctgggc aatccaggac ctgggggtta
     2221 cggcgctatt ttacgctatc gcggacgcga gaaaaccttt agcgcaggct acacccgcac
     2281 caccaacaac cgtatggagt tgatggccgc tattgtcgcg ctggaggcgt taaaagaaca
     2341 ttgcgaagtc attttgagta ccgacagcca gtatgtccgc cagggtatca cccagtggat
     2401 ccataactgg aaaaaacgtg gctggaaaac cgcagacaaa aaaccagtaa aaaatgtcga
     2461 tctctggcaa cgtcttgatg ctgccctagg gcaacatcaa atcaaatggg aatgggttaa
     2521 aggccatgcc ggacacccgg aaaacgaacg ctgtgatgaa ctggctcgtg ctgcggcaat
     2581 gaatcccaca ctggaagata cgggctacca agtcgaagtt taagcttgtg gtttacgaca
     2641 ttgccgggtg gctccaaccg cctggcgaat tcgtggcttg tttttactct gtttcatcgg
     2701 gtttagagtt aaaggaatag tccgtttccg ggcaacaata agttgtaagc aaccaagcgc
     2761 aggaatatgc gcattcaata gttttcctcc gtgtttgttc cacgggagaa cgtggaaacg
     2821 gctggcgtgc agcacttcaa aattcaacaa agagagccag tccagttgcc gcatcagagt
     2881 aaacatccgg ctgttatagg gcgaggtttt tcgcaatacc ggcacaagtt tgcgtaatcc
     2941 cattaaacta atgggattga agccactaat caccagccag ccatcatcaa tcaatacccg
     3001 atcggtttca cgcaataaac gatgcggatc ggtgcaccac ggcaatgtat gtgccagtag
     3061 acaaacatca acggatttat cggcaaaagg aagatgaagt ggatccgcct gcacctggac
     3121 gggcattcct tgcgcagaaa cattcacttg atgagaaacc gcgcacgctt cgcaattgat
     3181 ttctgcgctt aaattgccaa tcttaagcag atgaaaacca tacattttag tgaaccacgg
     3241 gttgagctgg cgctccagcg ccttgcgata aagctctccc cagggcaaat cgccccagcg
     3301 atcaggagcc acgacagttt gagggactct tgccggtttc atcacaacct tccgtttcac
     3361 actaagaggt aatctatgaa tcttaacagt attcccgcct ttgatgacaa ttacatctgg
     3421 gttttgaatg atgaagcagg tcgctgcctg attgtcgatc ccggagacgc agagccagta
     3481 ttaaacgcca ttgccgccaa taactggcaa ccggaggcca tatttctcac ccaccatcat
     3541 cacgatcacg ttggcggcgt aaaagaactg gtggaaaagt tgccgcaaat tgtggtgtat
     3601 ggtccacaag agacacaaga taagggaaca acacaggtag tcaaagatgg cgaaactgcc
     3661 ttcgttttgg ggcatgaatt tagtgtaatt gccacaccgg gtcacacttt aggacacatc
     3721 tgttacttca gtaaacctta tctattttgc ggcgacacgc tgttttctgg tgggtgtggt
     3781 aggttgtttg aagggacagc atcacaaatg tatcaatcac ttaataagtt aagtgcgtta
     3841 cctgacgata cattggtatg ttgtgctcat gaatatacgt tatcaaatat gaagtttgct
     3901 ttgagtattc ttccgcacga tttgtccata aatgattatt atcgtaaagt taaggagtta
     3961 cgggcaaaaa atcaaataac actacccgta attctgaaaa atgagcggca aattaatgtt
     4021 tttttaagaa cggaagatat tgatttaatt aatgtaatta atgaagaaac attattgcaa
     4081 caacctgaag agcgttttgc atggttaagg tcaaagaaag ataggttctg ataaaacttt
     4141 cttgtcatcg gctccgttcg ccgttatgat cggtcgtctt ttaagcaact attgacacac
     4201 acatgaaggc aaaagcgata ttactcgcct ctgtcctgct cgtgggttgc cagagtaccg
     4261 gcaacgttca acagcacgca cagagccttt ctgcagctgg tcaaggggaa gcagcaaagt
     4321 ttacaagtca ggcacgatgg atggacgatg ggacgtctat cgcgccagat ggtgacttgt
     4381 gggctttcat tggcgacgag ctaaagatgg gaattccgga aaatgaccgg attcgcgaac
     4441 agaaacagaa atatttacgc aataagagct atctccacga tgtaacttta cgggcagagc
     4501 cgtatatgta ctggatagca gggcaagtta aaaaacgtaa catgcctatg gaactggtac
     4561 tactacccat agtggagagc gcttttgatc cttacgcaac gtctggcgcc aatgccgcag
     4621 gcatctggca gatcattccg agcacggggc gcaattatgg tttgaaacag acccgcaatt
     4681 atgacgcgcg tcgcgatgtt gttgcttcaa caactgccgc gctgaatatg atgcagcgtc
     4741 tgaacaaaat gtttgacggc gactggcttc tgaccgtagc ggcttataac agcggcgaag
     4801 gtcgggtcat gaaggcaatt aaaacgaaca aagcgcgtgg gaaatccacg gacttctggt
     4861 cgttaccgtt gccgcaggaa acgaagcagt acgtgcctaa aatgctggca ttgagtgata
     4921 ttctcaaaaa cagcaagcgt tatggcgtac gtctgccaac gaccgatgaa agccgtgctc
     4981 tggcgcgtgt gcacctgagt agcccggttg aaatggcgaa ggttgcagat atggcgggga
     5041 tttccgtcag caagctgaag acattcaacg ctggcgtgaa agactccacg ctgggcgcaa
     5101 gtgggccgca gtacgtgatg gtgccaaaga agcatgcaga tcaactgcgt gaatctctcg
     5161 cttcaggcga aattgctgct gtacagtcga cgctggttgc cgacaatacg ccgcttaaca
     5221 gccgtgttta caccgtacgc tctggcgaca cgctttcaag catcgcttca cgtctcggcg
     5281 taagcaccaa agatttgcag cagtggaaca aactgcgcgg atctaagctg aagccaggcc
     5341 aaagtttgac gattggtgca ggcagtagcg cacagcggtt ggcaaacaac agcgatagca
     5401 ttacgtatcg tgtgcgcaaa ggcgattcgc tttcaagcat tgctaaacgc cacggcgtga
     5461 acatcaaaga tgtgatgcgc tggaacagcg acactgcgaa tctgcaacca ggcgataagc
     5521 tgacgttgtt tgtgaaaaac aacaacatgc cagattcctg acaaaccaga taataaaaaa
     5581 ggcaccgatt cccccggtgc cttttttatt tatgccgctt tatgtgcttc taccatgatg
     5641 atatcactgg tgaaagagcc atcagtctgc aaggaaaaat acgttctcac ctctgtcgat
     5701 gcgctctgtt ggtaaatgcg aatagcgtct actaacgctt ctggcgtacg cattctcacg
     5761 acccatgaag aaaattccag cggtaactta tctgtaatta aattatcaac tatcagattg
     5821 gcttcattga ttaacgtcaa ccacgcaccg ctggcgtagt ttcgtacgtg agaggtatcg
     5881 cgtaatgctt ctaccgcctg taaccagatg tcgcgcactg ggtgacccgg agacattaca
     5941 tccatcacaa tcagcctacc gccaggtttc aatatcctat tcacttctcg cagtgctgca
     6001 ccaacatcat gccagtgatg ggcagaataa cggctgataa caatatcaaa tgcgttatcg
     6061 gcaaatggca gacttgcggc atatccctgg cgggtggtga tatttttcag ttgccgggct
     6121 tcggcagctt gtgccacgac atccagcatg tgggcagata agtcatacgc caccaccgcg
     6181 ctcacgtttt gcgcagcgcc aaacgttggt ctgccctgtg gcagacctga cataccttat
     6241 ttatcaggct tgccgggact gaattcaacg agtagcggat tgtgatcgga agcgcgcgta
     6301 accagtacag aagcttcact gacgttcaga ccacggtaga aaacaaaatc gagcgggcga
     6361 ccaaacgccc gacggcgctg atcatcggta aaacgcacct ggcgcagcga catttcccgc
     6421 gcaaagcgat ataacgcgtt catccttcta cggctccagg cattgaaatc tcccgccata
     6481 atgaccgggc cgctgtggtg agctatctga tcaccaatag gaagtaactg cttactatag
     6541 acatccacgc ccagactgaa gttgacggcg tgtatattaa ccaccatcaa caggcgggtg
     6601 tcaggtaatg gatagaccgt caccagtgcc gacttcgcca gacgcaaaat gggttctcgt
     6661 tcacgtaacg ggcagcaata cactggatgt gccgccgaaa gggtcattac gccagaagga
     6721 tgttgtggca gcacgaaagc gggtacctga tcggcggcaa gatagttagc ggtcgcaaac
     6781 tgtactaact ctggcgttgt ctgcgcttcc tgcaataaca ccagatgtgc atctttgccg
     6841 tagttcttta ataccgacaa ccattcagcg cgttgctgtt tgtatatgtt ccacaccagg
     6901 atccgaatac gctcttcggt acttaacggt tccccaggtg gtaatgcctg gccgatgctc
     6961 gcaaaagacc ccggcggtaa gatcctttcc gcaggttgtc cggcaacata gcgcatggca
     7021 taggtgtttt ttcgcacttt tgactttgaa acctctatta cgtcaaccct tccgcactcg
     7081 ggagggttct actgttatag ggattttagc tcactctttc aacgagcaat tgtatattgt
     7141 tatgtaagca agtgcttaca ccggtcagaa ttttgctcat tatggagaac attaaatcgc
     7201 atgacatatc acgcatcctg atggtttaat gacaaataaa ttcaaaatat ctcttctatt
     7261 ttgcaacagg agcaaaaatg aaagccacgt cggaagaact cgccattttt gtttcggtcg
     7321 tcgaaagcgg cagctttagc cgggcagcgg tacaattagg gcaagcaaac tcagcggtaa
     7381 gccgggcggt gaaaaagctg gagatgaaac ttggcgttag cctgcttaat cggaccacgc
     7441 gacaacttag cctgacggaa gaaggcgagc gttattttcg tcgcgtacag tcaattttgc
     7501 aggagatggc agcggcagaa tcagaaatta tggagacgcg taatacaccg cgtggactgt
     7561 tacggatcga tgccgcaact ccagtggtgc tgcactttct gatgccgtta attaagcctt
     7621 tccgtgaacg ctatccggaa gtcactttgt cgctagtctc ctccgaaacg attattaatt
     7681 tgatcgaaag aaaagtggat gtcgcgatac gcgctggtac gttaacggat tccagcttac
     7741 gtgccaggcc gttatttaac agttatcgaa aaattatcgc ctcccccgat tatatttccc
     7801 gctacgggaa accagaaacg atcgacgatt taaagcaaca tgtttgcctg ggattcactg
     7861 aacccgcttc cctcaatacc tggccgatag cctgtagtga tggacaatta catgaggtga
     7921 agtacggttt gtcatccaat agtggggaaa cactgaaaca gctttgcctg agtggcaacg
     7981 ggattgcgtg tttgtccgac tacatgatcg acagagaaat cgctcgcgga gaattggtgg
     8041 agttaatggc agataaagtg ttgccagtgg aaatgccatt cagtgccgtc tattacagcg
     8101 accgtgcggt aagtacgcgc atccgggctt ttatcgattt ccttagcgag catgtaaaaa
     8161 cagctcccgg aggagctgtc agagaggctt aatcccattc aggagccaga ccttccgggc
     8221 taaccaggcg gtcgttgcaa tccagtgcgg cgatcgcttt tttatcttcg gcatcaagct
     8281 gtaaattttg tgccttaaga ttactttcca ggtttttacg tttagtagaa gaaggaatta
     8341 ctgagtaacc ttcccccata gcccacgcca gaatcacttg tgccggagtc gcattgtgtt
     8401 tagctgcgat acgagcaata acctcatctt tcagggcctt accatacgcc agcgtcatat
     8461 aggaagtaat atggatgccg tgctgtttag cccaggcaac cactttacgg ttttgcagat
     8521 aaggagagag ttcaatctgg ttagtagcga tgttttcagc gccaacagca gcaatcgcct
     8581 tttccatcaa tgggatcgtg aagttggaaa taccgatctc acgcgtcagc ccttgttttt
     8641 tggcttccag cagcgcctgc ataaactctt caacagagac ttcatcgttt ggtgacggcc
     8701 agtggattag cgtcagatca acataatcgg tacgcaattt ttgcaggctc tctttcagac
     8761 ttgggatcaa tttgtctttg ctgagatttt caatccagat tttagtggtg atgtagagtt
     8821 catgacgtgg cacgccactt tctgcaatcg cctgacctac tgcggcttcg ttatcataga
     8881 tttgtgcggt atcaattgcg cgataaccaa gttcaagcgc cgttttcaca gatgaaataa
     8941 caacgtcgtc tttcagacgg aaagtaccta aaccaaatgc agggatagcc ataatatgcc
     9001 tcttttaatg ctattttcgt taactgaggt gattatgggc ttcagactgt tgaggaaaaa
     9061 gagcgtaaaa tgcagaggat ttttgcgatt ctggcaataa tagatataca gacgaaaaaa
     9121 aacctcgagc gttaactcga ggcttcttaa taagtggcgg aacggacggg actcgaaccc
     9181 gcgcccccct gcgtgacagg caggtattct aaccgactga actaccgctc caccgaattc
     9241 ttttacaacc accggtttta tgaccggctt actgcttaat ttgatgcctg gcagtttatg
     9301 aatcacttcg tgattcaccc ttcgggccgt tgcttcgcaa cgttcaaaag ctttcgcttt
     9361 tgtcctactc tcttatcgag tagtgaactg cctcaaaggt cagtgccatt cttc
//
LOCUS       DAZKHC020000142         9403 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZKHC020000142 DAZKHC020000000
VERSION     DAZKHC020000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.142"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            71..223
                     /gene="hokE"
                     /locus_tag="ACHOSM_RS21260"
                     /old_locus_tag="pgaptmp_004252"
     CDS             71..223
                     /gene="hokE"
                     /locus_tag="ACHOSM_RS21260"
                     /old_locus_tag="pgaptmp_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGU4560623.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            574..726
                     /gene="hokE"
                     /locus_tag="ACHOSM_RS21265"
                     /old_locus_tag="pgaptmp_004253"
     CDS             574..726
                     /gene="hokE"
                     /locus_tag="ACHOSM_RS21265"
                     /old_locus_tag="pgaptmp_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGU4560624.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(848..1468)
                     /gene="entD"
                     /locus_tag="ACHOSM_RS21270"
                     /old_locus_tag="pgaptmp_004254"
     CDS             complement(848..1468)
                     /gene="entD"
                     /locus_tag="ACHOSM_RS21270"
                     /old_locus_tag="pgaptmp_004254"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HGU4560625.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1505..1603)
                     /locus_tag="ACHOSM_RS21275"
                     /old_locus_tag="pgaptmp_004255"
                     /pseudo
     CDS             complement(1505..1603)
                     /locus_tag="ACHOSM_RS21275"
                     /old_locus_tag="pgaptmp_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1643..3883)
                     /gene="fepA"
                     /locus_tag="ACHOSM_RS21280"
                     /old_locus_tag="pgaptmp_004256"
     CDS             complement(1643..3883)
                     /gene="fepA"
                     /locus_tag="ACHOSM_RS21280"
                     /old_locus_tag="pgaptmp_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HGU4560626.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            4126..5328
                     /gene="fes"
                     /locus_tag="ACHOSM_RS21285"
                     /old_locus_tag="pgaptmp_004257"
     CDS             4126..5328
                     /gene="fes"
                     /locus_tag="ACHOSM_RS21285"
                     /old_locus_tag="pgaptmp_004257"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HGU4560627.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5331..5549
                     /gene="ybdZ"
                     /locus_tag="ACHOSM_RS21290"
                     /old_locus_tag="pgaptmp_004258"
     CDS             5331..5549
                     /gene="ybdZ"
                     /locus_tag="ACHOSM_RS21290"
                     /old_locus_tag="pgaptmp_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HGU4560628.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            5546..>9403
                     /gene="entF"
                     /locus_tag="ACHOSM_RS21295"
                     /old_locus_tag="pgaptmp_004259"
     CDS             5546..>9403
                     /gene="entF"
                     /locus_tag="ACHOSM_RS21295"
                     /old_locus_tag="pgaptmp_004259"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HGU4560629.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINLAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLVQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPI"
BASE COUNT         2032 a         2485 c         2603 g         2283 t
ORIGIN      
        1 cctggggcta cctacccaaa cctgaacaat ttggatggta gtcccttctt cgcatggagg
       61 caatataaac atgctgacga aatatgccct tgcggcagtc atagtgctgt gtttaacggt
      121 gctgggattt acgcttctgg tcggggattc gctgtgtgag ttcaccgtaa aggaacgtaa
      181 tattgagttt aaggctgttc tcgcttacga accgaagaag tagccgttgt gcggggagca
      241 atccccgcat atccggttgt caggtcagga tggtaaggca cctgctttac actttcgccc
      301 gcggtcagtg atggctgcgg gcgatttcgt tttaagagca atgatgagtt ttgccgacag
      361 aagtccgaaa caggattcac tgtataaata aacagctatt ttgttgagga agggtaagat
      421 aacggcgggt gcctgaagct ttccggtttc aggttaactc tgaggtctgg aaagatgaag
      481 ccccaggaga tatttctatc aatcctgggg ctgccactcc gagcctcaac aactcggatg
      541 gtagtccctt cttcgcatgg aggcaatata aacatgctga cgaaatatgc ccttgtggca
      601 gtcatagtgc tgtgtttgac ggtactggga tttacgcttc tggtcggaga ctcgctgtgt
      661 gagttcaccg taaaggaacg taatattgag tttaaggctg ttctcgctta cgaaccgaag
      721 aagtagccgt tgtgcgggga gcaatccccg catatccggt tgtcaggtca gagtggtaag
      781 gcacctgctt tacactttcg cccgcggtca gtgatggctg cgggcgaatc gtaccagatg
      841 ttgtcaatta atcgtgttgg cacagagtta tgactatttt ttcttttatc tgccagtgca
      901 cagcaaacat ctcattctca cgatgaatga tgacctgctg tttattccag ctaattatct
      961 gatagtccag aaaacctgca tcagtttgga tctcacttgc cttaaatgcg ctctctttgg
     1021 cggaaaatgc cagtgtcagc gccagagaaa aggctaaacc gcagtctgcg agtcgctcgt
     1081 gttctgctgg tgtaataatg ttgtctgtca attcggtcgc tgtttgcgca gaaaaaattt
     1141 cttcgatatc aacgccaatc ggttgacggg aaaccacggc caatgccgta gccccgcagt
     1201 ggctaatact gccgtatacc tccgcaggcc acaccggttg gcgtagctcg ccgattgcgg
     1261 gcacacattt atagccatat tcccgcagcg cataaattgc agcgatccgc ccggctaaat
     1321 gttcggcttt gcgcttccgt ccagcgtgtt gcagttgcgc gtaatgcggc agccagagta
     1381 aatcctgctc acaaaaattc gccggatcga actcaacaaa atgcagcgta tgtccggcaa
     1441 aggggaggga ggtatgcgta gttttcatgt cgaccatatt agagactgat gacaaacgct
     1501 aaactgcctg atgcgctacg cttatcaggc ctacatggtc ctgcaatata ttgaattggc
     1561 aagatttttg taggccggat aaggcgttca cgccgcatcc ggcatgaacg acgcgcactt
     1621 tgtcaacaat ctgacgttag catcagaagt gagtgtttac gctcatgtac cacgtacgtc
     1681 ccggctcgtt ataggtatac gccccggcac ccgcgatata gttggcccct gccagatcgc
     1741 ccgtggtctg ggcattaccc gcacgccaca aacgtttgtc gaacaggttg tccacgccgc
     1801 cagtcagact gacattcttc gtcacatccc aggtcgcgct cagacccaca atgctgtaag
     1861 gactgatttc tttggtttcc gggccaaccg ctggctgacc tttgtagttg tattttttcg
     1921 gctgctgctt accgtaccag gtgaaggtcg tttgcatcga caaatcttcc cgcgcctgcc
     1981 agctcagcgt tgagttcaac gtgtactccg ggatgatcga caaacggtcg cccgtggttt
     2041 tgttttcact cttcagcata taagtgatgt tattggtcca catcaccgtt tcgctaactg
     2101 gtacgtttaa cgatccttcc agaccttcaa ccactgcttt cggcacgtta tcccactgat
     2161 agagatcggt gccgactgcg ttttgcccta cagccacata gcccgcttca atcttattgc
     2221 gataatcgtt acggaaccag gtgacgcccg ccagccagcc gtcgcgtttg aactccagac
     2281 caatctcttt gttgatgctg gtttctgctt tcaggtcatc gttaccttgc agatagcagc
     2341 cgcccgcgct ggcatagcag ccctgacctt tactgtagag aatgtagttc gggttagtct
     2401 ggtacaggct cggtgcttta taagcacggg caatgcccat tttgagcgtg aagtcatcgc
     2461 ctaaaccttg cgatatgttc agcgccgggc tccagttatt gccgacaata ctgtgatgat
     2521 cgaaacgcag ccccggcgtt acgatggtgc tgtcagtcag ctccatgttg ttttcggcaa
     2581 acagcgagaa aatttctgct tttgaatacg ggctacggtc ggtggcactc acaccatcaa
     2641 tagcgccgcc ggtgttcgcc ccggtcagcg cctgggtatt ggaactcaag tccttcatcc
     2701 gttgctgatt ccactccgta cccagcgtca gcgtctggtt aacgaggaaa tcaatcggca
     2761 ggttaacttc gctgtgcagc atcacgtcat cgagatcgat atcgacgaaa tcctgtgtcg
     2821 ctttttcgtt aaatttccct tcggtaccgc ccgccagacc ttccggaata cgcgagttac
     2881 gggtgtgttc atactgcacc cagttgctgg tggtcacgcc gttatcccag ccaccgttcc
     2941 aggttagcgc gtagttctgg cgatacaggc ggttggtttc atcgccatat ttcgagcgag
     3001 tgtaagcgtc agagttggtg ttctgggtat cgcccgcata caggttaccc tggcggctgt
     3061 aacccgcttc cagttccagc gattgcaatg gtgcgaagtc ccagcgcacc acgccgttaa
     3121 tatctttatt gatcacccct tcgcgcccgg ctggtaacgt cgtggcatac gttccggcac
     3181 gcgcggactg atggccctgg ttgatatccc acgcgtcagc ctgggttttg tcgaggttgc
     3241 catacaagcg gaagctgaat tcgtcgccca gcgggccggt cagactgaag ttggtgcgtt
     3301 tagtggcacc ttcctcttta tgttctggcg cattgaaata agcgtcccag gaaccgtgcc
     3361 actcaccgct gccttttttg gtaatgatgt taactacgcc acccgccgcg ccgttgccat
     3421 aacgcgctgc agccggacca cgcagaactt caatacgttc aatcatttca ggcggcaccc
     3481 aggaagtatc accacgggta tcgcgctcgc cacgccagcc ctgacgcacc gagttacggc
     3541 tgctgaccgg tttgccgtca attaaaatca gcgtgttttc cggacccata ccgcgaatat
     3601 caatctgtcg gttattccca cgctgaccac tggtggagtt accggtcagg ttaacgcctg
     3661 gcatggtacg gatgatctcc gacacatcgc gggcaaccgg gtttttgcgg atttcatctg
     3721 cggtgatggt cgaaacgcca ggcgcctgta agttctgctc ggcggcggta acgacaatag
     3781 tatcgtcatg tgaaacagga gtatcggtcg gctcttgtgc ctgcgctacc ccataaatcc
     3841 ccagattgac caacaaggcc agggaatgaa tcttcttgtt cattgtttta ttcctgcatt
     3901 tttgccacga attgcaactg tcgggcatgg tcgtcatcaa cacgacgcat cccgctaccg
     3961 cgaaaacctt tgatcctgaa agacacgcag tgcagttggt taattaatgt ccgcgcttcc
     4021 cacggcgcgc cattacgcta ttgcaaatgc aaatagttat caataatatt atcaatatat
     4081 ttctgcaatc aatgaaaaat tgcacagtaa acatggggtt atggtgtgac ggcgttaaaa
     4141 gtaggaagtg agagctggtg gcagtcgaaa catggcccgg aatggcagcg tctgaatgac
     4201 gaaatgtttg aggtcacttt ctggtggcgt gatccccaag gttctgaaga atactcgacg
     4261 ataaagcgcg tatggatcta catcactggt gtgaccgatc atcatcagaa cagccagccc
     4321 cagtcgatgc agcgaattgc aggtactgat gtctggcagt ggacgacaca actcaatgcc
     4381 aactggcgcg gcagctactg ctttattccc accgaacgcg atgacatttt ttctgcacca
     4441 tcccccgatc gcctcgaatt acgtgaaggc tggcgaaaac tattacccca ggcaatagcc
     4501 gatccgctga atccacaaag ctggaaaggc gggcgagggc acgctgtttc tgcactcgaa
     4561 atgccgcaag cgcctctgca accgggatgg gattgtccgc aagcgccaga aataccagcc
     4621 aaagaaatta tctggaaaag tgaacggttg aaaaattcac gtcgtgtatg gatttttacc
     4681 accggcgatg taacagcaga agaacgcccg ctggcagttt tgctcgatgg cgaattttgg
     4741 gcgcaaagta tgcccgtctg gcctgtgctg acttcgctga cccatcgtca gcaacttcct
     4801 cccgccgtgt atgtgttgat cgacgctatc gacaccacgc accgcgccca cgaactgccg
     4861 tgtaatgcgg atttctggct ggcagtacag caagagttat tacccctggt gaaagttatt
     4921 gcccctttta gcgatcgcgc cgatcgcacc gtggtcgccg ggcagagttt tggtgggctg
     4981 tccgcgctgt atgccggact gcactggcct gaacgctttg gctgtgtatt aagccagtca
     5041 ggatcgtact ggtggccgca tcggggcggg cagcaagagg gcgtgttact tgaaaagcta
     5101 aaagctggtg aagttagcgc cgaaggtctg cgcattgtgc tggaagcggg tattcgcgag
     5161 ccgatgatca tgcgggccaa tcaggcgctg tatgcgcaat tacaccctat aaaagaatcc
     5221 attttctggc gtcaggttga cggcggacat gatgcgcttt gttggcgcgg tggcttgatg
     5281 caggggttaa tcgacctctg gcaaccactt ttccatgaca ggagttgaat atggcattca
     5341 gtaatccctt cgatgatcca cagggagcgt tttacatttt gcgcaatgcg caggggcaat
     5401 tcagtctgtg gccgcaacaa tgcctcttac cggcaggctg ggacattgtg tgtcagccgc
     5461 agtcacaggc gtcctgccag cagtggctgg aagtccactg gcgtactctg acaccggcca
     5521 atttcaccca gttgcaggag gcacaatgag ccagcattta cctttggtcg ctgcacagcc
     5581 cggcatctgg atggcagaaa aactgtcgga tttaccctcc gcctggagcg tggcgcatta
     5641 cgttgagtta actggagatg ttgatgcgcc attactggcc cgcgcggtgg ttgccggact
     5701 agcgcaagca gatacgttgc ggatgcgttt tacggaagat aacggcgaag tctggcagtg
     5761 ggtcgatgat gcgctgatat tcgaactgcc agaaattatc gacctgcgaa ccaatattga
     5821 tccgcacggt actgcgcagg cattaatgct ggcggatttg caacaagatt tgcgcgtcga
     5881 tagcggtaaa ccactggtct ttcatcagct gatacatgtt gcggataacc gctggtactg
     5941 gtatcagcgt tatcaccatt tgctggtcga tggcttcagt ttcccggcca ttactcgcca
     6001 gatcgccaat atttactgca catggctgcg tggcgaacca acgcctgctt cgccgtttac
     6061 gcctttcgct gatgtagtgg aagagtacca gcaataccgc gaaagcgaag cctggcagcg
     6121 tgatgcggca ttctgggcag aacagcgtcg tcaactgccg ccgcccgcgt cactttctcc
     6181 ggcaccttta ccggggcgca gcgcttcggc agatattctg cgcctgaaac tggaatttac
     6241 cgacggggaa ttccgccagc tggctacgca actttcaggt gtgcagcgta ccgatttagc
     6301 ccttgcgctg gcagccttgt ggctggggcg attgtgcaat cgcatggact atgccgccgg
     6361 atttatcttt atgcgtcgac tgggctcggc ggcgctgacg gctagcggac ccgtgctcaa
     6421 cgttttaccg ttgggtattc acattgcggc gcaagaaacg ctgccggaac tggcaacccg
     6481 actggcggct caactgaaaa aaatgcgtcg ccatcaacgt tacgatgccg aacaaattgt
     6541 ccgtgacagc gggcgagcgg caggtgatga accgctgttt ggtccggtac tcaatattaa
     6601 ggtatttgat taccaactgg atattcctgg tgttcaggcg caaacccata ccctggcaac
     6661 cggtccggtt aatgaccttg aactggccct gttcccggat gaacacggtg atttgagtat
     6721 tgagatcctc gccaataaac agcgttacga tgagccaacg ttaatccagc atgctgaacg
     6781 cctgaaaatg ctgattgccc agttcgccgc ggatccggcg ctgttgtgcg gcgatgtcga
     6841 tattatgctg ccaggtgagt atgcgcagct ggcgcagatc aacgccactc aggttgagat
     6901 tccagaaacc acgctgagcg cgctggtggc agaacaagcg gcaaaaacac cggatgctcc
     6961 ggcgctggca gatgcgcgtt acctgttcag ctatcgggaa atgcgcgagc aggtggtggc
     7021 gctggcgaat ctgctgcgtg agcgcggcgt taaaccaggg gacagcgtgg cggtggcact
     7081 accgcgctcg gtctttttga ccctggcact ccatgcaata gttgaagctg gcgcggcctg
     7141 gctaccgctg gataccggct atccggacga tcgcctgaaa atgatgctgg aagatgcgcg
     7201 tccgtcgctg ttaattacca ccgacgatca actgccgcgc tttagcgatg ttcccaattt
     7261 aacaagcctt tgctataacg ccccgcttac cccccagggc agtgcgccgc tgcaactttc
     7321 acaaccgcac cacacggctt atatcatctt tacctctggc tccaccggca ggccgaaagg
     7381 ggtaatggtc gggcagacgg ctatcgttaa ccgcctgtta tggatgcaaa atcactaccc
     7441 gcttacaggc gaagatgtcg ttgcccaaaa aacgccgtgc agttttgatg tctcggtgtg
     7501 ggagtttttc tggccgttta tcgcaggggc aaaactggtg atggctgaac cggaagcgca
     7561 ccgtgacccg ctcgctatgc agcaattctt tgccgaatat ggcgtaacga ccacgcactt
     7621 tgtgccgtcg atgctggcgg catttgttgc atcgctgacg ccgcaaaccg ctcgccagaa
     7681 ttgcgcgacg ttgaaacagg ttttctgtag tggtgaggcc ttaccggctg atttatgccg
     7741 cgaatggcaa cagttaaccg gcgcgccgtt gcataacctg tatggcccga cggaagcggc
     7801 ggtagatgtt agctggtatc cggcttttgg cgaggaactg gcacaggtgc gcggcagcag
     7861 tgtgccgatt ggttatccgg tatggaatac gggtctgcgt attcttgatg cgatgatgca
     7921 tccggtgccg ccgggtgtgg cgggtgatct ctatctcacc ggcatccaac tggcgcaggg
     7981 gtatcttgga cgacccgatc tgaccgccag ccgttttatt gccgatcctt ttgccccagg
     8041 tgaacggatg tatcgtaccg gagacgtcgc ccgctggctg gataacggcg cggtggagta
     8101 cctcgggcgc agtgacgatc agctaaaaat tcgcgggcag cgtatcgaac tgggcgaaat
     8161 cgatcgcgtg atgcaggcgc tgccggatgt caaacaagcc gttacccacg cctgtgtgat
     8221 taacctggcg gcagccaccg gtggtgatgc gcgtcagttg gtgggctatc tggtgtcgca
     8281 atcaggtctg ccgttggata ccagcgcatt acaggcacag cttcgcgaaa cattgccgcc
     8341 gcatatggtg ccggtggttc tgctgcaact tccacagtta cctcttagcg ccaacggcaa
     8401 gctggatcgc aaagccttac cgttgcctga actgaagaca caagcgtcgg gacgtgcacc
     8461 gaaagcgggc agtgaaacga ttatcgccgc ggcattcgcg tcgttgctgg gttgtgacgt
     8521 gcaggatgcc gatgctgatt tctttgcgct gggcggtcat tcgctactgg caatgaaact
     8581 ggcagcgcag ttaagtcgac agtttgctcg tcaggtgacg ccggggcagg tgatggtcgc
     8641 gtcaaccgtc gccaaactgg caacgattat tgatggtgaa gaggacagct cccggcgcat
     8701 gggattcgaa accattctgc cgttgcgtga aggtaatggc ccgacgctgt tttgtttcca
     8761 tccggcgtcc ggttttgcct ggcagtttag cgtgctctcg cgttatctcg atccacaatg
     8821 gtcgattatt ggcattcagt cgccgcgccc tcatggaccc atgcagacag cgacaaacct
     8881 ggatgaagtc tgcgaagcgc atctggcaac gttacttgag caacaaccgc acggccctta
     8941 ttacctgctg gggtattccc ttggcggtac gctggtgcag ggtattgcgg cgcgactgcg
     9001 tgcccgtggc gaacaggtgg catttcttgg cttgctggat acctggccgc cagaaacgca
     9061 aaactggcag gaaaaagaag ctaatggtct ggacccggaa gtgctggcgg agattaaccg
     9121 cgagcgcgag gccttcctgg cggcacagca gggaagtact tcaacggagt tgtttaccac
     9181 cattgaaggc aactacgctg atgctgtgcg cctgctgacg actgctcata gcgtaccgtt
     9241 tgacggtaaa gcgacgttgt ttgttgctga acgcacgctt caggaaggta tgagtcctga
     9301 acgcgcctgg tcgccgtgga ttgcggagct ggatatctat cgtcaggatt gtgcgcatgt
     9361 ggatattatc tctccagggg cgtttgtaaa aattgggccg att
//
LOCUS       DAZKHC020000143         9284 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZKHC020000143 DAZKHC020000000
VERSION     DAZKHC020000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.143"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..496
                     /gene="ehxC"
                     /locus_tag="ACHOSM_RS21300"
                     /old_locus_tag="pgaptmp_004260"
                     /gene_synonym="hlyC"
     CDS             <1..496
                     /gene="ehxC"
                     /locus_tag="ACHOSM_RS21300"
                     /old_locus_tag="pgaptmp_004260"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HGU4560630.1"
                     /translation="VMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFALLIKDEL
                     PVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMELYKYMRKK
                     YPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNRQDFNFNI
                     EKEN"
     gene            498..3494
                     /gene="ehxA"
                     /locus_tag="ACHOSM_RS21305"
                     /old_locus_tag="pgaptmp_004261"
     CDS             498..3494
                     /gene="ehxA"
                     /locus_tag="ACHOSM_RS21305"
                     /old_locus_tag="pgaptmp_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HGU4560631.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGSLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARIISELGDKDDEVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKVDKIPHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            3544..5664
                     /gene="ehxB"
                     /locus_tag="ACHOSM_RS21310"
                     /old_locus_tag="pgaptmp_004262"
                     /gene_synonym="hlyB"
     CDS             3544..5664
                     /gene="ehxB"
                     /locus_tag="ACHOSM_RS21310"
                     /old_locus_tag="pgaptmp_004262"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HGU4560632.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELSAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            5668..7107
                     /gene="ehxD"
                     /locus_tag="ACHOSM_RS21315"
                     /old_locus_tag="pgaptmp_004263"
     CDS             5668..7107
                     /gene="ehxD"
                     /locus_tag="ACHOSM_RS21315"
                     /old_locus_tag="pgaptmp_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HGU4560633.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVNSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESLRER"
     gene            7174..7299
                     /locus_tag="ACHOSM_RS21320"
                     /old_locus_tag="pgaptmp_004264"
                     /pseudo
     CDS             7174..7299
                     /locus_tag="ACHOSM_RS21320"
                     /old_locus_tag="pgaptmp_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7299..7460
                     /locus_tag="ACHOSM_RS21325"
                     /old_locus_tag="pgaptmp_004265"
                     /pseudo
     CDS             7299..7460
                     /locus_tag="ACHOSM_RS21325"
                     /old_locus_tag="pgaptmp_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
     gene            complement(7510..7863)
                     /gene="cmi"
                     /locus_tag="ACHOSM_RS21330"
                     /old_locus_tag="pgaptmp_004266"
     CDS             complement(7510..7863)
                     /gene="cmi"
                     /locus_tag="ACHOSM_RS21330"
                     /old_locus_tag="pgaptmp_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HGU4560634.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQKMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GIYNCEEGYVEYAND"
     gene            complement(8035..8817)
                     /locus_tag="ACHOSM_RS21335"
                     /old_locus_tag="pgaptmp_004267"
     CDS             complement(8035..8817)
                     /locus_tag="ACHOSM_RS21335"
                     /old_locus_tag="pgaptmp_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGU4560635.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVNALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNKHA"
     gene            complement(8819..9265)
                     /locus_tag="ACHOSM_RS21340"
                     /old_locus_tag="pgaptmp_004268"
     CDS             complement(8819..9265)
                     /locus_tag="ACHOSM_RS21340"
                     /old_locus_tag="pgaptmp_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560636.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELIFEDGSDALELIFEDGSDAPFVIHMLSEQC
                     DRLLPENNQGGGFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
BASE COUNT         2857 a         1653 c         2091 g         2683 t
ORIGIN      
        1 cgtcatgggg aaggttgcat ggctatgggc ctgttctcct ctgcataaaa aatggccatt
       61 atctgttttt gctattaatg tcatacctgc tattcagaca aatcaatttg ctctcttaat
      121 aaaggatgag cttcctgtag ctttttgtag ctgggccagt ttagatctgg aatgtgaggt
      181 aaaatatata aatgatgtaa catctttgta tgcaaaagac tggatgtcgg gagaaagaaa
      241 gtggtttatt gactggatag ctccatttgg acacaacatg gagttatata aatacatgag
      301 gaaaaagtat ccttatgagt tattcagagc aataaggtta gatgagagca gtaaaacagg
      361 aaaaatagct gaatttcatg gaggggggat agataaaaaa ctggcatcaa aaatattcag
      421 gcaataccat catgaactta tgtctgaagt aaaaaacaga caagatttta attttaatat
      481 tgagaaagaa aactaatatg acagtaaata aaataaagaa cattttcaat aatgcgacat
      541 tgactacaaa atcagcattt aatacagcat catcaagcgt acgttccgct ggcaaaaaac
      601 tcatattatt aatacctgat aattatgaag ctcagggcgt gggtattaat gagttggtca
      661 aagctgctga tgagcttgga atagaaatac accgtactga acgagatgat acagcgattg
      721 caaaccagtt ttttggtgca gcagaaaaag ttgtaggatt aactgaacgt ggtgttgcaa
      781 tattcgcacc acaacttgac aaacttctgc agaagtatca gaaagttggg agtaaaatag
      841 gaggaaccgc tgaaaatgta ggtaataatc tgggaaaagc cggaacagtt ctctcagcac
      901 tacagaattt tacggggatt gctttatcag gcatggctct tgatgaattg ctgagaaaac
      961 aacgggaagg cgaggatata agtcagaatg atattgccaa aagtagtatt gaacttatta
     1021 atcagcttgt agatacagta tcaagtataa acagtaccgt tgattcattt tctgagcagc
     1081 ttaaccagct tggctcattt ttatccagta aacctcgctt aagttctgtt ggtgggaaat
     1141 tacaaaattt accagacctg ggctccctgg gggatgggct ggatgttgtc tccggaattc
     1201 tttctgctgt atcagcaagc tttattctgg gaaacagtga cgcacataca ggaacaaaag
     1261 ctgcagcggg tatcgaactg acaactcagg ttcttggaaa tgttggtaaa gctgtttcgc
     1321 aatatattct ggctcagaga atggcacagg ggttatcgac aacagctgca agtgcgggtc
     1381 tgatcacatc ggctgttatg ctggctatca gtcctctttc tttcctggct gctgcagata
     1441 aatttgagcg agctaagcag cttgaatcat attctgaacg atttaaaaaa ttgaattatg
     1501 aaggggatgc tttactcgca gcctttcata aagaaaccgg agctatagat gcagccctga
     1561 caacaataaa tactgtcctg agttctgtat ctgcgggagt tagtgcagcc tccagtgcat
     1621 ccctcatagg ggccccgata agcatgctgg tgagtgcatt aaccggtacg atatctggca
     1681 ttctggaagc atcaaaacag gctatgtttg agcacgttgc agagaaattc gctgctcgga
     1741 tcaatgaatg ggaaaaggag catggcaaaa attattttga gaatggctat gacgcaagac
     1801 atgctgcgtt tttagaagac tctctgtctt tgcttgctga tttttctcgt cagcatgcag
     1861 tagaaagagc agtcgcaata acccagcaac attgggatga gaagatcggt gaacttgcag
     1921 gcataacccg taatgctgat cgcagtcaga gtggtaaggc atatattaat tatctggaaa
     1981 atggagggct tttagaggct caaccgaagg agtttacaca acaagtgttt gatcctcaaa
     2041 aagggaccat agacctttca acaggtaatg tatcaagtgt tttgacattt ataacaccaa
     2101 catttacccc aggagaagaa gttagagaaa gaaaacagag tggtaaatat gaatatatga
     2161 catctcttat tgtaaatggt aaggatacat ggtctgtaaa aggcataaaa aatcataaag
     2221 gtgtatatga ttattcaaaa ttgattcagt ttgttgaaaa gaataacaaa cactatcagg
     2281 cgagaataat ttctgagctc ggagataaag acgatgaggt ttattctgga gcaggctcat
     2341 cagaagtatt tgctggtgaa ggtcatgata ccgtatctta taataagacg gatgttggta
     2401 aactaacaat tgatgcaaca ggagcatcaa aacctggtga gtatatagtt tcaaaaaata
     2461 tgtatggtga cgtgaaggta ttgcaggaag tcgttaagga acaggaggtg tcagtaggga
     2521 agcgaacaga gaaaatacaa tatcgtgatt ttgaattcag aaccggtgga attccttatg
     2581 atgtaataga taatcttcat tctgttgaag agctcattgg cggaaaacat gatgatgaat
     2641 tcaaaggcgg taagtttaat gatatattcc atggcgcaga tgggaacgat tatatcgaag
     2701 gtaattatgg taatgatcga ctatacggcg atgatgggga tgattatata tccggaggac
     2761 agggagacga ccagttattt ggtggtagtg gaaacgataa attgagtgga ggggatggta
     2821 ataattatct gacaggagga agcggtaatg atgagcttca ggcacacgga gcttataata
     2881 ttctgtcagg tggtactggt gatgataaac tttatggtgg tggtggtatt gatcttctgg
     2941 atggagggga aggtaatgac tatctgaatg gtggttttgg taatgatatt tatgtttata
     3001 ggcaaaacta tggtcatcat acaattgcag atgaaggagg taaaggagat cgtttgcact
     3061 tatctgatat tagctttgat gatatcgcat ttaagagagt tggaaatgat cttatcatga
     3121 ataaagccat taatggtgta ctttcattta atgagtcaaa tgatgtcaat gggataacat
     3181 ttaaaaactg gtttgcgaaa gatgcctcag gagcagataa tcatcttgtt gaggttataa
     3241 cagataaaga tggtcgagag ataaaagttg ataaaatacc tcataataat aatgaacggt
     3301 caggttatat aaaagccagt aatatagcat ctgaaaaaaa catggttaat atcaccagtg
     3361 ttgccaatga tattaataag attatttctt cagtttcagg gttcgattca ggtgatgaac
     3421 gattagcatc tttatataat ttatccttac atcaaaacaa cacacactca acaactttaa
     3481 cgacaacggt ctgagactaa aagaggaggt gatggccatt ccaggaggaa tggtcatcgt
     3541 tatatgatga gtaaatgtag ttctcataat agtctgtatg cactgatatt gcttgcacaa
     3601 tatcataata taactgtcaa tgctgaaact ataaggcatc agtataatac ccacacacaa
     3661 gattttgggg tgactgaatg gttactggca gcgaaatcca ttggcttaaa agcaaaatat
     3721 gtagaaaaac atttttccag attgtcaata atttctttac ctgcgttgat atggcgggat
     3781 gacggtaagc attatatatt gtctcgtgtt actaaagatt catcacgcta tcttgtttat
     3841 gatccagaac aacatcagtc actaactttt agtcgggatg agtttgaaaa actgtatcag
     3901 ggaaaagtca ttctggttac gtcaagagca acagtagtcg gagagttagc taaatttgat
     3961 ttttcttggt ttatcccctc tgttgtgaaa tacaggagga ttttacttga ggtgttaact
     4021 gtttctgctt ttattcagtt tcttgcgtta ataacacctc ttttttttca ggttgtaatg
     4081 gataaggttt tagttcaccg ggggttttca acgttaaata ttatcacaat agcatttatt
     4141 atagtgatac tttttgaagt gatattaacc ggagccagaa cttatatttt ctctcatact
     4201 acaagtcgta ttgacgtcga actgagcgct aagttattca gacatttgct tgcattgcct
     4261 gtttcatatt ttgaaaatcg cagggtcgga gagaccgttg ccagagtaag ggaactggag
     4321 caaattcgta attttttaac cggacaagcg ttgacatcag ttcttgatct atttttttct
     4381 gtaatatttt tttgtgtcat gtggtattac agccctcaat taacactggt tatattattg
     4441 tcactacctt gttatgttat atggtcattg tttatatcac ccttattacg tcgacgtctt
     4501 gatgataagt ttctcaggaa tgcagaaaat caagcttttc ttgtcgaaac ggtaacagca
     4561 ataaatacaa ttaaatccat ggcagtatca ccacagatga ttgctacatg ggataaacaa
     4621 ctggccgggt atgttgcttc cagtttcagg gtaaatctgg ttgcaatgac agggcagcag
     4681 gggatacagc tgatacagaa aagtgtaatg gtaattagtc tatggatggg agcacatctt
     4741 gtcatatcgg gagagataag catcgggcag ttaatcgcgt ttaatatgct tgcaggtcag
     4801 gtcattgctc ctgttatcag actggctcat ctttggcagg attttcagca ggttggtatt
     4861 tctgttgagc gccttggtga tgtattaaat acaccggtag agaaaaagtc aggcagaaat
     4921 atactgccgg aaattcaggg ggatatcgaa tttaaaaatg tcagatttcg gtattcttct
     4981 gacggtaatg ttattttgaa taatattaat ttatacatat caaaggggga tgttatcggt
     5041 atagttggcc gttctggttc aggaaaaagt acattaacaa aactgcttca gcgcttttat
     5101 ataccagaga ccggacagat tttaattgat gggcatgatt tatcacttgc agatccagaa
     5161 tggttacgac gccagattgg tgttgtattg caggaaaata tactattaaa tcgtagtatt
     5221 atcgataata ttacattagc ttctccggct gtatctatgg aacaggctat tgaggcagcc
     5281 agacttgcag gtgcccatga ttttattaca gaactaaaag aagggtacaa tactattgtt
     5341 ggagaacagg gcgttggtct ttcaggaggg cagcgccaac ggatcgcaat agcccgggct
     5401 cttgttacaa atcctcgaat tcttattttt gatgaagcaa ccagtgctct tgattatgag
     5461 tcggaaaata taataatgaa aaatatgtca agaatatgta agaacagaac cgtaattatt
     5521 attgcacaca ggttgtcaac tgtaaaaaat gcaaacagaa taattgttat ggataatggc
     5581 tttatttctg aagatggcac acataaagag cttatctcca aaaaagacag tttatatgca
     5641 tatttatatc agttgcaggc ataacgtatg cgcttttaca tgaaaggttt atgggatttg
     5701 gtttgccgtt ataagacggt tttttctgat gtctggaaaa ttcgtcacac acttgatgct
     5761 cctgtgagag aaaaagatga gtatgcattt cttcctgctc accttgaact cattgaaaca
     5821 ccagtatcca gacgttctca ttttgttgta tggagtattt tattatttgt aattatatct
     5881 cttctcttat ctgttcttgg gaaagttgaa gtggtttcag tagcaaatgg taagtttact
     5941 catagtggaa gaagcaaaga aataaaaccg atagagaatg cgattgtaga aaaaataatg
     6001 gtaaaagatg gctcttttgt aaaaaaaaat gatccattag ttgaattgac ggtgcctggt
     6061 gttgaatctg atattttaaa atcagaagca tctttgttgt atgaaaaaac agaacaatat
     6121 cgctacgcaa ttctttctga atcaatacaa cggaatgagc ttcctgaaat aaggataaca
     6181 gattttcctg gcggagaaga taatgcggga ggcgaacatt ttcagagggt taactcatta
     6241 ataaaagagc aattcatgac atggcagaac agaaaaaatc aaaagcagct cacattaaat
     6301 aaaaaaatag ttgaacggga tgcagcgctt gctcgtgtta gcctgtatga gcatcaggta
     6361 tcacaggaag gaagaaaact caatgatttt aagtatttgt tgaataaaaa agctgtttct
     6421 caacattcag ttatggagca ggaaaatagc tatattcagg caaaaaatga acatgcagtc
     6481 tggcttgcac aggtttctca acttgaaaaa gaaatagaac ttgtgcggga agaactggca
     6541 ctggagacga atatcttcag aagtgaaatt atcgagaagc acagaaaatc aacagataac
     6601 attgtgttgc tggagcatga gcttgaaaaa aacaggcaga gaaaagcatc atcttttatt
     6661 aaagctcctg tgagtggtac tgttcaggag ttaaatatac atacagaagg tggtgttgtc
     6721 acaacagcag aaacgctgat gattattgtc cctgataatg atattctcga agtaacagca
     6781 tctgtactta acaaggatat cggttttata caacctggac aggaggtcgt tattaaagta
     6841 gatgcatatc catatacacg tcatggttac cttacaggga aagtaaaaaa tataactgca
     6901 gattctgttt ctgttccgga tacagggctt gtatttaacg tgattatatc tgttgatcgg
     6961 aatgatatac agggagaaag aaaaaaaatc cctgttacag ctggaatgac tgttatggca
     7021 gaaataaaaa ccggggtgcg tagtgttatc agctatcttc ttagtccatt aaaggaaact
     7081 attaatgaaa gtttacgtga acgttagtat ataaataaca tattggcttt ttatatgata
     7141 tagatttctg aaatgatctt gtaaagatta tctatgttat ttaaacaaac tatgatgcag
     7201 tttttatctt taaaaagagt taatggaatg gatagtatta aattacttga taaagttatt
     7261 atctatcaac aacagaatga gcaattatat ccagcacagg cgttgttcgt tcatatgatg
     7321 ataaatacga ttttaacgcc agcactcacc gtggcatcat cggagagtcg ctcacaaggc
     7381 tcggggcgat gttttctggt aaagagtacc agatactgct tcctggtgaa attcacatta
     7441 aagaaagtgg taagcgataa tataagggcc gggattccgg cctttttatg cagcctttat
     7501 aacagtgtat tagtcattcg catattcaac atatccctcc tcacaattgt atatgccata
     7561 atacattctc ttaccctccg ggccttcagc tttaaatggg acaagaagtg ttttttcatc
     7621 ttcaacatct gttaaacttc tctcaatctt ttcccatgta attttcggga tttctgtacc
     7681 aagaagttga acatcgttgt cccagcgttt cattttttgc aggtaatcat tttttacaaa
     7741 cgttctggcc tgttcatcgc tgacctgata gcatgccgga cctttatctt cagaccagaa
     7801 aaaaacagca gcaagagcaa taatcgttaa aataaaaata aatttcatgc taattacttt
     7861 cataaaatct ccacaacttc tgtcgttcat tcgatataaa aaacattacg aatatatccg
     7921 tacaatgtga gcatactgtg aagtggcttt aacaattatg aaataataca atagatcaca
     7981 tattgcagtg gtctcttttt tatagaagaa atcacaattc ataattgtga cttattatgc
     8041 atgtttgttt ttcagcattg tgaccgcatc ggactcaggc atcgaaaact gcacccggtg
     8101 ccgggcagcg acatccagcg caaacacctt cgtgtagacc tccgttgagc tgatggactt
     8161 atgccccatc agactctgca ggaccttcag cggtataccg gcatacagca tatgcatcgc
     8221 ataggaatgg cggaacgtat gtggcgtgac cgggacagag aacgtcacac catcagcggc
     8281 agcggcggca accgcctccc caatccaggt cctgaccgtt ctgtccgtca cttcccagat
     8341 ccgcgctttc tctgtcctgc ctgttcgttt attacgccgt tccatgggga ttttcaatgt
     8401 ggccaccatc gtctgcagct ggctgacgta ccaggagtcg gagagcggaa ccagccggtg
     8461 agtctgctgc ccggcgggcg ttcttcctgc cgtcctggcg gctttttcgg tccgttgttt
     8521 cagggtcgca agctgcacaa acggatacgg aggcgtgagc gaaaaatccc cccgcgtcag
     8581 cgccagtgct tcattaatgc gcgcaccggt gttccacagt gtggccagca gcatcttgcg
     8641 gcgcagatcc gggacgtaat ggagcagggc gttcacttca ggggccagca ggtatttggg
     8701 cagttcatca tgaaccatcg acatctggcg gagtgccagc gctgccggat aatcgatggc
     8761 aacaggaagc tgagcggcat cccgggcatg gtggatcggt gccggcaggt gctgcatatc
     8821 agtgttcact ccacggggaa acgtcgggca ggttttccac aacccggtac tttcccggat
     8881 aacggagctg gttaccgcca cgcgtccaga cggtgacaac aaaaccccct ccctggttgt
     8941 tttcggggag caggcgatcg cactgctcac tcagcatgtg gatcacgaac ggcgcatcgc
     9001 tgccgtcttc aaatatcagt tccagcgcat cgctgccgtc ttcaaatatc agttccagcg
     9061 catcgcggcc atgcagcgtt cccttactga tgatgacgta ctcagccccc cgcatctccc
     9121 tgagtaaatg ttttgccgca tccgggacaa gcagcctggc tgcgccggca ttccagctga
     9181 ggtagacata tccggcctgc gcctgtacag actgccagta atccgttctg gcgacagcct
     9241 gaccttcatt ctcaataaaa aacatgggtg actccttttt tctt
//
LOCUS       DAZKHC020000144         9253 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZKHC020000144 DAZKHC020000000
VERSION     DAZKHC020000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.144"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..334
                     /locus_tag="ACHOSM_RS21345"
                     /old_locus_tag="pgaptmp_004269"
     CDS             <1..334
                     /locus_tag="ACHOSM_RS21345"
                     /old_locus_tag="pgaptmp_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560637.1"
                     /translation="DVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSS
                     QRLHDMLNPTKDTNWNSTCIYKSRHKMLPVNLTQETLFNSKSQDKHALFPIFTASWRA
                     YRIMNKGA"
     gene            557..1054
                     /gene="ubiC"
                     /locus_tag="ACHOSM_RS21350"
                     /old_locus_tag="pgaptmp_004270"
     CDS             557..1054
                     /gene="ubiC"
                     /locus_tag="ACHOSM_RS21350"
                     /old_locus_tag="pgaptmp_004270"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HGU4560638.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1067..1939
                     /gene="ubiA"
                     /locus_tag="ACHOSM_RS21355"
                     /old_locus_tag="pgaptmp_004271"
     CDS             1067..1939
                     /gene="ubiA"
                     /locus_tag="ACHOSM_RS21355"
                     /old_locus_tag="pgaptmp_004271"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HGU4560639.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFIV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2094..4517)
                     /gene="plsB"
                     /locus_tag="ACHOSM_RS21360"
                     /old_locus_tag="pgaptmp_004272"
     CDS             complement(2094..4517)
                     /gene="plsB"
                     /locus_tag="ACHOSM_RS21360"
                     /old_locus_tag="pgaptmp_004272"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HGU4560640.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMSLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4688..5056
                     /gene="dgkA"
                     /locus_tag="ACHOSM_RS21365"
                     /old_locus_tag="pgaptmp_004273"
     CDS             4688..5056
                     /gene="dgkA"
                     /locus_tag="ACHOSM_RS21365"
                     /old_locus_tag="pgaptmp_004273"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGU4560641.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5166..5774
                     /gene="lexA"
                     /locus_tag="ACHOSM_RS21370"
                     /old_locus_tag="pgaptmp_004274"
     CDS             5166..5774
                     /gene="lexA"
                     /locus_tag="ACHOSM_RS21370"
                     /old_locus_tag="pgaptmp_004274"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HGU4560642.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5847..7172
                     /gene="dinF"
                     /locus_tag="ACHOSM_RS21375"
                     /old_locus_tag="pgaptmp_004275"
     CDS             5847..7172
                     /gene="dinF"
                     /locus_tag="ACHOSM_RS21375"
                     /old_locus_tag="pgaptmp_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HGU4560643.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7288..7497
                     /gene="yjbJ"
                     /locus_tag="ACHOSM_RS21380"
                     /old_locus_tag="pgaptmp_004276"
     CDS             7288..7497
                     /gene="yjbJ"
                     /locus_tag="ACHOSM_RS21380"
                     /old_locus_tag="pgaptmp_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGU4560644.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7539..8054)
                     /gene="zur"
                     /locus_tag="ACHOSM_RS21385"
                     /old_locus_tag="pgaptmp_004277"
     CDS             complement(7539..8054)
                     /gene="zur"
                     /locus_tag="ACHOSM_RS21385"
                     /old_locus_tag="pgaptmp_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HGU4560645.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8372..8626
                     /gene="yjbL"
                     /locus_tag="ACHOSM_RS21390"
                     /old_locus_tag="pgaptmp_004278"
     CDS             8372..8626
                     /gene="yjbL"
                     /locus_tag="ACHOSM_RS21390"
                     /old_locus_tag="pgaptmp_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HGU4560646.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            8650..>9253
                     /locus_tag="ACHOSM_RS21395"
                     /old_locus_tag="pgaptmp_004279"
     CDS             8650..>9253
                     /locus_tag="ACHOSM_RS21395"
                     /old_locus_tag="pgaptmp_004279"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HGU4560647.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYQGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLI"
BASE COUNT         2221 a         2162 c         2444 g         2426 t
ORIGIN      
        1 tgatgtttca cctatattta tagaaactga agaaaagtta agaaccttta acactaataa
       61 attaacacga aacgataata tatttatcct gttctcctct gtcgatgatg ggcctgttat
      121 ggtggtaagc tcccagcgct tacatgatat gttgaatcct acaaaagata ccaattggaa
      181 ttccacgtgt atctataaat ccagacataa gatgttgcct gttaatctta ctcaggaaac
      241 acttttcaac tccaaatctc aggataaaca tgcgcttttc cccattttta ctgcgagttg
      301 gcgagcttat cgtataatga ataagggtgc ttaagtaaag gaaaacatca ccgttcctgg
      361 catcatgggc ggtgatgccc ctacggttgc cctcgccagc acgggcatcg gcaaagcgta
      421 aggttcaaca gcggtttacc acttcatgcg attgttgcgt ttttgttgcg cattagatca
      481 cttaatttgc tttacatctc ccgtaaacac ttttctgcga tacaatgcct ttacgttatg
      541 taacggagag ttcggcatgt cacaccccgc gttaacgcaa ctgcgtgcgc tgcgctattt
      601 taaagagatc cctgccctgg atccgcaact gctcgactgg ctgttgctgg aggattccat
      661 gacaaaacgt tttgaacagc agggaaaaac ggtaagcgtg acgatgatcc gcgaagggtt
      721 tgtcgagcag aatgaaatcc ccgaagaact gccgctgctg ccgaaagagt ctcgttactg
      781 gttacgtgaa attttgttat gtgccgatgg tgaaccgtgg cttgccggtc gtaccgtcgt
      841 tcctgtgtca acgttaagcg ggccggagct ggcgttacaa aaattgggta aaacgccgtt
      901 aggacgctat ctgttcacat catcgacatt aacccgggac tttattgaga taggccgtga
      961 tgccgggctg tgggggcgac gttcccgcct gcgattaagc ggtaaaccgc tgttgttaac
     1021 agaactgttt ttaccggcgt caccgttgta ctaagaggaa aaaaatatgg agtggagtct
     1081 gacgcagaat aagctgctgg cgtttcatcg cttaatgcgt acggataagc caattggcgc
     1141 gttactgctg ctctggccaa cattatgggc gttgtgggtg gcgacaccgg gcgttcctca
     1201 gctctggatc ctggcggtat ttgtcgcggg tgtctggctg atgcgcgctg ccggatgtgt
     1261 ggtgaatgat tatgctgacc gcaagtttga tggtcatgtt aagcgcacgg cgaaccgacc
     1321 acttcccagc ggcgcggtaa cagagaaaga ggcgcgcgcg ctgtttatcg tgctggtact
     1381 gatttcgttt ttactggtgc tgacgctgaa tacgatgacc attctgttgt cgattgccgc
     1441 tctggcgctg gcgtgggtgt acccgtttat gaagcggtat acccatctac cgcaagtggt
     1501 gctgggcgcg gcgtttggct ggtcgattcc aatggctttt gccgctgtga gtgagtcggt
     1561 gccattgagt tgctggttaa tgttcctcgc caatattctc tgggcggtgg cttacgacac
     1621 gcagtatgcg atggttgacc gcgatgatga tgtgaagatt ggcattaaat ccacggcaat
     1681 cctgttcggc caatacgata aattgattat tggtattttg cagattggcg tactggcact
     1741 gatggcgatc atcggtgagt taaatggctt aggctgggga tattactggt caattctggt
     1801 ggctggcgcg ctgtttgttt atcaacaaaa actgattgcc aaccgcgagc gtgaagcctg
     1861 ctttaaagca tttatgaata ataactatgt tggtctggta ctatttttag ggctggcaat
     1921 gagttactgg catttctgat gatgtaaaaa agccggatga tcatccggct ttcttctggg
     1981 ttgcctgatg cgcggcgctt ctcaggccta cacaacacat cgcaatttat tgaatttgca
     2041 gattatggaa ggccggataa ggcgttttcg ccgcatccgg caattctctc tgattaccct
     2101 tcgccctgcg tcgcactctc aatcgtcaaa cgcacgtctg atgtaatcaa ctccgccagc
     2161 aactgataaa ccttcatcgt ttctgccggt tcggcatcgc cgctatcgct gatataccct
     2221 tcatcacgca gtgtcagcac cagagaactg aacaccgcct tgtcgaagaa ctccggcgcg
     2281 ttgatgccgt gcagcacgga gagacgttgc gcgacggtgc ggctctcttt ctccagcgta
     2341 ccgcggttga tcgacgggtt ggcactcaac aaccagaagg tgatggcata acgttgcagc
     2401 gtttcgcgcg cgcctgcggc cagcagctgt agcgtgcgag aatgcgccgg gttgatatgc
     2461 aactcatcat cttgcagggt aatcagcccc tgacgttgca tctcatttgc cagcgcatca
     2521 ataacgtccg gcaactcgtc gcgatcccag cgcaggaaca gctccgcttt cagcattggg
     2581 taaagcacat tgacgtgctc catcaatacg tcgcgggaga tgtggcgatg ctgggtgacg
     2641 attgccgcca tcagcgaagg cagcaccaac atatgcgcaa tgttgttgcg atagtaggtc
     2701 atcagcaccg cttgctcgcg cggcagaatg atgatgtcgc cgattgtgtc tttctcgact
     2761 tcaaacttgt tcatttgcag cgcgtgatcg ataagctcgc tggcgctggc tgaaggaacg
     2821 gtagagtccg tggagtaagg tacgttgcgc atcagatcca ggtagcagtt gagttgctcg
     2881 gttaactgct cgcgggtgag tgagcgctga cgtgacgcca gaagcgcagt acagcacagg
     2941 ctcatggcgt ttgccgcgcc tgcgttgtta atgcgtacca tcagatcggc agcaatatta
     3001 ttgaccgtcg gcgttaacca tgccggacgc accgcttcga tgggatcgat agattcacgc
     3061 cagtctggta cgtgctggtt aaggtaggtc atcaacggca ttggttcacc gaagttgacg
     3121 taaccctgac cgagattacg cagcttgctt aaaccgcgca gcatctgcgg caggctctct
     3181 ttctctttcg tcgcgccgcg cagttctttg gcgtaagtac ccacttccat gacgtgctca
     3241 taaccgatgt agatcggaat cagcgtaatc ggacgcgtgc cgccacgcag catcgcctga
     3301 atggtcatcg acagcgtacc ggttttcgga tccagcaaac gccccgtgcg ggaacgaccg
     3361 ccttccacga agtactcgac ggaataacca cggctgaaca gttcgccgag atactcacgg
     3421 aaaacggtgg aataaagttt attgccttta aaagtacggc gaataaagaa cgcccccaga
     3481 cggcggaaaa tcggcccggc cggccagaag ttcaggttga tcccagcggc gatatgcggc
     3541 ggcaccagcc cctggtgata cagcacgtaa gaaagcagca ggtagtccat gtggctgcgg
     3601 tggcaaggca catataccag ctcatgaccg tcgtgggcca gctggcgaac gcgctcggcg
     3661 ttatggacgt tgatgccctg gtaaaggcgg ttccaggtga agcccagaat acggtcagtc
     3721 aggcgaatca tctcgtaaga gaaattcgcc gcaatctctt ccatcagcgc aatcgcgttc
     3781 tgctgcgctt tttcatggga gatttttttg ctgcgcgctt catcttctac cgctttggca
     3841 atggcgcggg aggcgagcag cttattaaac agatcctgac gagcaggcag acgtgggccc
     3901 acagcagcca gacgttgacg ggcaaagtgc atacgcgcca cgcgcgccag tttctgtgcg
     3961 atagttttat ccgtgccgtg ttcatccgcc atacggcgca gcgaaactga cggcgagaaa
     4021 cgcacaaaac tgtcgcgacc gagccacagt acggcgaaaa atttctgtac gccgttaagc
     4081 atacgcagcg gcgggtttac ttcgcctttt tcacgccccg gcgcgcgacc aaacatcacc
     4141 gatactggca ccatctgcac atccagattt gggttgctac ggtgcaaatc gagatagtcg
     4201 tggaacagct taatagactc ttctttcggc gtgtaatagg tgaatacacg cggcccgccg
     4261 tgaatgaaca catagcgcgg cagtagcgtg ccgtcgattt ccagcggctc taacgggtca
     4321 ggcaagtcat gagccagaca ctgggcgcgt aacgtcagca aatctgcttt cgagttgtac
     4381 ggtaaaacgt acataattgg acgagaagta tccagcccca gttccggggc aggatctgcc
     4441 ggaatagact tgctttttac caggatgctt aatggtaaat tcagtaattt gtagtaaatt
     4501 cgtggccagc cggacataaa cgatgtaaag cctctggtta ataatgcaaa tgcgcggcaa
     4561 ggatagcaga aagtcatggg aaattctgtg gtatccgctc atgtttcgcg cggcgttacg
     4621 caaacccgaa tcatcggatt taacggtaca ctgatattga cgctcataat gtaaaaaggt
     4681 tctttcaatg gccaataata ccactggatt cacccgaatt atcaaagctg ctggctattc
     4741 ctggaaaggt ttacgcgctg catggatcaa cgaagcggca ttccgtcagg aaggcgtagc
     4801 ggtattgttg gcggtggtca tcgcctgctg gctggatgtg gacgcaatta cccgcgtgct
     4861 gcttatcagc tccgtgatgc tggtgatgat tgtggaaatc ctcaatagcg ccatcgaagc
     4921 agtggttgac cgaattggct ctgaatacca tgagctttcc ggacgcgcaa aagatatggg
     4981 atccgccgcg gtgctgattg cgattatcgt cgccgtgatt acctggtgca ttctgttatg
     5041 gtcgcatttt ggataaccct tccagatttc gataaatctc tggtttattg tgcagtttat
     5101 ggttccaaaa tcgccttttg ctgtatatac tcacagcata actgtatata cacccagggg
     5161 gcggaatgaa agcgttaacg gccaggcaac aagaggtgtt tgatctcatc cgtgatcaca
     5221 tcagccagac aggtatgccg ccgacgcgtg cggaaatcgc gcagcgtttg gggttccgtt
     5281 ccccaaacgc ggctgaagaa catctgaagg cgctggcacg caaaggcgtt attgaaattg
     5341 tttccggcgc atcacgcggg attcgtctgt tgcaggaaga ggaagaaggg ttgccgctgg
     5401 taggtcgtgt ggctgccggt gaaccgcttc tggcgcagca gcatattgaa ggtcattatc
     5461 aggtcgatcc ttccttgttc aagccgaatg ctgatttcct gctgcgcgtc agcgggatgt
     5521 cgatgaaaga tatcggcatt atggatggtg acttgctggc agtgcataaa actcaggatg
     5581 tacgtaacgg tcaggtcgtt gtcgcacgta ttgatgacga agttaccgtt aagcgcctga
     5641 aaaaacaggg caataaagtc gaactgttgc cagaaaatag cgagtttaaa ccaattgtcg
     5701 ttgaccttcg tcagcagagc ttcaccattg aagggctggc ggttggggtt attcgcaacg
     5761 gcgactggct gtaacatatc tctgagaccg cgatgccgcc aggcgtcgcg gtttgttttt
     5821 catctctctt catcaggctt gtctgcatgg cattcctcac ttcatctgat aaagcacttt
     5881 ggcatctcgc cttacccatg attttctcca atatcaccgt tccgttgctg ggactggtcg
     5941 atacggcggt aattggtcat cttgatagcc cggtttattt gggcggcgtg gcggttggtg
     6001 caacggcgac cagctttctc tttatgctgt tgctgttttt acgcatgagc accaccgggc
     6061 tgactgcgca ggcttatggt gccaaaaatc ctcaggcatt agcccgtgcg ctggtgcaac
     6121 cgttgctgtt ggcattgggg gctggggcgt taattgcgct gctgcgtaca cctattatcg
     6181 atctggcgct gcatattgtt ggcggtagtg aagcagtcct ggaacaggcg cggcgctttc
     6241 ttgaaatccg ctggttaagc gcaccggcgt cgctggcgaa tctggtatta ctcggttggt
     6301 tactcggcgt gcaatatgcc cgtgcgccag taattttgtt agtggtcggc aacatcctca
     6361 acattgtgct ggatgtctgg ctggtgatgg ggctgcatat gaacgtgcag ggcgcggcgc
     6421 tggcgacggt tattgcggaa tatgcaacat tgctgattgg tctgctaatg gtgcgtaaaa
     6481 tcctcaaact acgcggaatt tccggcgaaa tgctgaaaac tgcctggcga ggaaacttcc
     6541 gtcgcttgct ggcgcttaac cgcgatatca tgctgcgttc gctgttgttg caactctgtt
     6601 tcggcgcgat caccgtactt ggtgcgcgac tggggagtga cattatcgct gttaacgcgg
     6661 ttctgatgac gctactcacc tttaccgcct atgcgcttga tggttttgcc tacgcggttg
     6721 aagcgcactc cggtcaggca tacggtgcgc gcgacggtag ccagttgctg gatgtatggc
     6781 gggcagcgtg ccgccagtcg gggattgtag cgttactgtt ttcggtggtt tatttgctgg
     6841 caggggaaca catcattgcg ttgctgacgt cgttaaccca gattcagcag ctggctgacc
     6901 gctatcttat ctggcaggtg attttgccgt tggttggcgt ctggtgttat ctgctggacg
     6961 gcatgtttat aggcgcaacg cgtgccgccg aaatgcgtaa cagtatggcg gtggccgccg
     7021 caggttttgc gctgacgctc cttacgctgc cgtggctggg taatcatggt ttgtggctgg
     7081 cattaaccgt ctttctggcg ttgcgcgggc tttctctggc ggctatctgg cggcgtcact
     7141 ggcgcaacgg tacctggttt gccgcaacgt gacggttaaa aattctgaat aaattatcct
     7201 aagccaaatt gctgactaca cttaatctca cgttcagaag aaaagtgaac gtactctcat
     7261 tcacaaccta acgatgaggt cttgattatg aataaagatg aagccggcgg taactggaaa
     7321 cagtttaaag gtaaagtgaa agagcaatgg ggcaaactga ccgatgatga tatgacgatc
     7381 attgaaggta aacgtgatca actggtcggt aaaatccagg aacgttatgg ttatcagaaa
     7441 gatcaggcag aaaaagaggt cgtggattgg gaaacccgca atgaatatcg ctggtaatta
     7501 atccctcctg cccgacgtgt acaaggatgt acgccctctt aacgcggttt ctttttcacc
     7561 tgcacagagt gatcatgctg gcactgttca ggatgacgac acgcttccac ttccacgcat
     7621 gccgcacaga gcccatgtgc ttcaatcaca ttatgccgca gggcaaaccc catttttgcc
     7681 gccagcgtat gcataatgtc ttccacgcct tctgcacact cttctttcac cgcgccgcag
     7741 cgatcgcata taaacatggc tgacgtatgc gtgggctggt cgaacagatg acagagcaca
     7801 taactgttgg tggattccac cttatgcaca aaaccttgct caagcagaaa atccagcgcg
     7861 cgataaaccg ttggcggctt ggcttgcggt tcagcttcgc gcagtaaatc aagcagatca
     7921 taagcgctga tagcgccatc ctgcagactc atcaggcgca acacttccag gcgctgtggg
     7981 gtcaggcgca cattacgctg cgcgcagatt ttttcagcct gcgctaataa ctcctgcgtt
     8041 gtggtctttt ccatcgggca cctcaaagtg ggggttaaga atgtccttac tttaccatgt
     8101 tccaggaaaa acaccgagaa cccttattgt tgccgtaatg ttgattttct gttttgtagg
     8161 taaggtgtta tgttgccttg tcgtaccatt atcaacacga taataattaa taattcattt
     8221 ttaaataaat tgcttcaagt taactcgcta tttatttaac tgggtttaaa cgcatacctg
     8281 tagttcagaa attatgagcg atttttgata gtgaccagat ggcaaaagaa ttataaggag
     8341 gacacggtac gacaaaaaag gtagatgaat catgttgaag ataatacccg gtgcaactgg
     8401 atactttaat aaaacactca attcaaatca attcgataat gaagatgcaa tcaaagataa
     8461 attagataat agaggttcaa tcaaaggtaa attaaataat atatatggta aatctattga
     8521 ttatgcagcg ctgcgtcatc gggatataat tattgcaaaa atagatttgt ttattcaacg
     8581 aatcacgcat aatttatggc atgcacgtaa aaaaatgtgt ttttaattga gcaaataaat
     8641 gatctcaaaa tgtgggtcaa taagtatatt gacgattgca ctgatgagga tttaaacgat
     8701 cgtgacttta ttgcatcagt tgttgaccgg gctatttttc atttcgcgat taatagtata
     8761 tgtaatcctg gggataataa agatgcgact cccattgaac aatgtacttt tgatgtagaa
     8821 actaagaatg gccttccctc cacggttcag ctattttatg aggaatctaa ggataatgaa
     8881 cctttagcga atatacattt tcaagcaata ggttctggtt ttttaacgtt tgttaatgcc
     8941 tgccaggaac atgatgacaa cagcttaaaa ttatttgctt cgctgttaat ttcactttca
     9001 tattctagtg cctacgcaga tttatcagaa acagtgtata ttaatgaaaa taatgagagc
     9061 tacctgaaag ctcagtttga aaaattatct caacgtgata tgaagaagta ccagggagag
     9121 atgaagcgtc tggctgatgg gggagaaatg aattttgatg gctatctgga taagatgtca
     9181 catctggtga atgaaggaac gctcgatcct gatattttaa gcaaaatgcg agatgctgca
     9241 ccacaattaa tta
//
LOCUS       DAZKHC020000145         9212 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZKHC020000145 DAZKHC020000000
VERSION     DAZKHC020000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.145"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2005)
                     /locus_tag="ACHOSM_RS21400"
                     /old_locus_tag="pgaptmp_004280"
     CDS             complement(<1..2005)
                     /locus_tag="ACHOSM_RS21400"
                     /old_locus_tag="pgaptmp_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HGU4560648.1"
                     /translation="MSTDKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRG
                     VVAMDDAGFDEEDFKKPVRVNLPIVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQDKPASSDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVLRISRTPSGANAGGGILTDRGEGFVHDDASVE
                     RDVAAGVLARSMDIDIYNPHPAHAKRIEEIVSENKPPFSVFRDKFIAMPGHLDYSRAI
                     VVASVKEAPIGIEATPHRVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTKE
                     GKQDDEEKPQPSGAMADEQATTEAVEPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAE
                     LHEARKNIPPQNPVDADKLLAASRGEFVEGISDPNDPKWIKGIQTRDSVYQNQPETEQ
                     NDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQRSGGNCPDCGAVMGDATYQ
                     ETFDDKNLVEVQEDDSEKMEGAEHPHKENAGSAQDHASDSETGETADPLITVNGHRII
                     TSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEF"
     gene            complement(2098..2286)
                     /locus_tag="ACHOSM_RS21405"
                     /old_locus_tag="pgaptmp_004281"
     CDS             complement(2098..2286)
                     /locus_tag="ACHOSM_RS21405"
                     /old_locus_tag="pgaptmp_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4560649.1"
                     /translation="MSSAFALMMTVFLITGEPQNVITGIYASKESCHQARDEQKISGE
                     CLPLNKVSLYLNNEIPAG"
     gene            complement(2283..2471)
                     /gene="dicB"
                     /locus_tag="ACHOSM_RS21410"
                     /old_locus_tag="pgaptmp_004282"
     CDS             complement(2283..2471)
                     /gene="dicB"
                     /locus_tag="ACHOSM_RS21410"
                     /old_locus_tag="pgaptmp_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4560650.1"
                     /translation="METSLPNVNTSEGCFNIGILLSNREFTEDAIRMRKYEPYLLNDN
                     SILSRIALLELGIFGGQQ"
     gene            2869..3033
                     /locus_tag="ACHOSM_RS21415"
                     /old_locus_tag="pgaptmp_004283"
     CDS             2869..3033
                     /locus_tag="ACHOSM_RS21415"
                     /old_locus_tag="pgaptmp_004283"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560651.1"
                     /translation="MNQLPRRALSRYGLTFQGNILSVNCQCRMLTRVRRTHSTSPVEN
                     YLITNLSFVG"
     gene            complement(3037..3255)
                     /gene="ydfC"
                     /locus_tag="ACHOSM_RS21420"
                     /old_locus_tag="pgaptmp_004284"
     CDS             complement(3037..3255)
                     /gene="ydfC"
                     /locus_tag="ACHOSM_RS21420"
                     /old_locus_tag="pgaptmp_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGU4560652.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     KLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(3285..3413)
                     /gene="ydfB"
                     /locus_tag="ACHOSM_RS21425"
                     /old_locus_tag="pgaptmp_004285"
     CDS             complement(3285..3413)
                     /gene="ydfB"
                     /locus_tag="ACHOSM_RS21425"
                     /old_locus_tag="pgaptmp_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HGU4560653.1"
                     /translation="MDFDTIMEKAYEEYFEGLADGEEALSFNEFKQALSSSAKSNG"
     gene            complement(3415..3570)
                     /locus_tag="ACHOSM_RS21430"
                     /old_locus_tag="pgaptmp_004286"
     CDS             complement(3415..3570)
                     /locus_tag="ACHOSM_RS21430"
                     /old_locus_tag="pgaptmp_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4560654.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(3737..4144)
                     /gene="dicA"
                     /locus_tag="ACHOSM_RS21435"
                     /old_locus_tag="pgaptmp_004287"
     CDS             complement(3737..4144)
                     /gene="dicA"
                     /locus_tag="ACHOSM_RS21435"
                     /old_locus_tag="pgaptmp_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4560655.1"
                     /translation="MDTKNLTIGERIRYRRKNLKYTQRSLAKVLKISHVSVSQWERDD
                     SEPTGKNLFALSKVLQCSPTWILFGDEDKQPSPPIEEPVALSPKEHELLELFNALPES
                     EQDAQLTEMRARVKNFNKLFEELLKARQRTNKR"
     gene            4228..4458
                     /gene="dicC"
                     /locus_tag="ACHOSM_RS21440"
                     /old_locus_tag="pgaptmp_004288"
     CDS             4228..4458
                     /gene="dicC"
                     /locus_tag="ACHOSM_RS21440"
                     /old_locus_tag="pgaptmp_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicB transcriptional regulator DicC"
                     /protein_id="HGU4560656.1"
                     /translation="MLKTDALLYFGSKTKLAQAAGIRLASLYSWKGELVPEGRAMRLQ
                     EASGGELQYDPKVYDEYRKAKRAGRLNNENHP"
     gene            4442..4993
                     /locus_tag="ACHOSM_RS21445"
                     /old_locus_tag="pgaptmp_004289"
     CDS             4442..4993
                     /locus_tag="ACHOSM_RS21445"
                     /old_locus_tag="pgaptmp_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4560657.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRITFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGSYEYRCTTMG"
     gene            4965..6005
                     /locus_tag="ACHOSM_RS21450"
                     /old_locus_tag="pgaptmp_004290"
     CDS             4965..6005
                     /locus_tag="ACHOSM_RS21450"
                     /old_locus_tag="pgaptmp_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HGU4560658.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRV
                     SLKVGKIGAVNKNSTENGYVSAQKSPKNGTLCCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPHVFLDGLSEPIGKFPMTDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELTAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            5998..6459
                     /locus_tag="ACHOSM_RS21455"
                     /old_locus_tag="pgaptmp_004291"
     CDS             5998..6459
                     /locus_tag="ACHOSM_RS21455"
                     /old_locus_tag="pgaptmp_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU4560659.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPLGPTPIELLKQEYERRKAAGFV"
     gene            6494..7264
                     /locus_tag="ACHOSM_RS21460"
                     /old_locus_tag="pgaptmp_004292"
     CDS             6494..7264
                     /locus_tag="ACHOSM_RS21460"
                     /old_locus_tag="pgaptmp_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4560660.1"
                     /translation="MGTVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAGEGESGVTEEQPAQSEVPDVLTGEVEQKVTADMMIEFICQDGAKTCEEL
                     ADKFGVSIRKVASTLAVVTATGSLARVNQNGKFRYCIPGADLPAEPEAASVTETDGKA
                     FPQPTGVALPVREAETQEEIKTESVAVTVQSQPLFTRKHPDGLILPSLHVANRELRRA
                     KGQVQKWERVCAALRELNKHRDIVRQIVDSSGRIVSEK"
     gene            7280..7675
                     /locus_tag="ACHOSM_RS21465"
                     /old_locus_tag="pgaptmp_004293"
     CDS             7280..7675
                     /locus_tag="ACHOSM_RS21465"
                     /old_locus_tag="pgaptmp_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU4560661.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKTTDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            7732..8088
                     /locus_tag="ACHOSM_RS21470"
                     /old_locus_tag="pgaptmp_004294"
     CDS             7732..8088
                     /locus_tag="ACHOSM_RS21470"
                     /old_locus_tag="pgaptmp_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560662.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERAYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRILRQEERERRRNIEKLIQIE
                     RNVKARIRRVTKSRTE"
     gene            8137..8349
                     /locus_tag="ACHOSM_RS21475"
                     /old_locus_tag="pgaptmp_004295"
     CDS             8137..8349
                     /locus_tag="ACHOSM_RS21475"
                     /old_locus_tag="pgaptmp_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560663.1"
                     /translation="MSNAQKVINAEKYNEWVKKFSEQIFKITGDENAAKNELEPWTPE
                     GVDPNYCWWDVDPVDAANEAMSYHND"
     gene            8385..8756
                     /locus_tag="ACHOSM_RS21480"
                     /old_locus_tag="pgaptmp_004296"
     CDS             8385..8756
                     /locus_tag="ACHOSM_RS21480"
                     /old_locus_tag="pgaptmp_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560664.1"
                     /translation="MTELTKEKLIEEAKLKIAIAKCYPNSGMARVEGELFKIALASLE
                     AEPIAWECGENIILFNPDTVEAYAKRAEISPKPLFSAPPALVVPDKLPREYRNGWPLA
                     YSDYAEGWNDCREAMLQGDKS"
     gene            8753..9115
                     /locus_tag="ACHOSM_RS21485"
                     /old_locus_tag="pgaptmp_004297"
     CDS             8753..9115
                     /locus_tag="ACHOSM_RS21485"
                     /old_locus_tag="pgaptmp_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4560665.1"
                     /translation="MINRIKLEHILEYARQQRHIGQHCKIPPGDMVEIMEIAMRKAGN
                     SPVTPDGWISCSERMPNTKTAVLVAVEFDRKGDWRMKLATYIPGHPDANDGWIIPGAS
                     WKPSHWMPLPEPPQEVRQ"
BASE COUNT         2380 a         2056 c         2410 g         2366 t
ORIGIN      
        1 taaattccgg tcccatatct ccggtttgcg gatcgaaaaa tattgcacct attgagataa
       61 tcggggcatc aggatttttt cccatggttt caaggtcgat cattagatgg tcacacgtcc
      121 tgctggtgga tgtgataatg cgatgaccgt tcaccgtaat taagggatct gccgtctcgc
      181 cagtttcact atcgctggcg tggtcctgag cgctgccagc attctccttg tgtggatgtt
      241 cagcgccttc cattttctcc gaatcgtctt cctgaacttc aaccaggttc ttgtcatcga
      301 atgtttcctg gtatgttgcg tcgcccatca ccgcgccaca atcagggcag ttgccgccac
      361 tcctctgacc gcaggcggtg cagatctttt ccggttcctg ttgcactact ggttcaggtt
      421 gtttcgtttc tggctcgttt tgttgcgtat ttgggctgtt ctgttccgct ttctggtcgt
      481 tctgttccgt ttctggctga ttctggtaca cagaatcgcg ggtctggatc cccttaatcc
      541 atttcggatc attcgggtcg ctaatccctt caacaaattc tccgcgagag gcagccagta
      601 atttgtctgc atcgacagga ttttggggcg gaatgttttt ccgggcttca tggagttctg
      661 cccgcagttt ctgatatttc gcatcaacag aatttatctg tggctgagca tccatcgact
      721 gcgtgtcctg attatgttca gttgtatccg gttccactgc ttcagtcgtt gcctgttcat
      781 ctgccattgc gccagatggc tgcggttttt cttcatcatc ctgttttcct tcttttgtta
      841 cacgctgcgg cattggagca gaggagcgac cgcaggcaat atccacgatt tctggatcag
      901 ggttggcatg gtcggtttcg gtcagtactt tgttcagata ttcggtaaca cggtggggag
      961 tagcctcgat accaattggt gcttctttca cggacgcaac cactatcgcg cgggaataat
     1021 ccaggtgacc aggcatggcg atgaatttgt cacgaaaaac agaaaagggc ggcttattct
     1081 ctgaaacgat ttcttcaatg cgtttggcgt gtgccggatg tggattgtaa atatcgatgt
     1141 ccattgaacg ggccagaacg ccagcggcaa cgtcacgttc tactgacgca tcatcgtgga
     1201 caaaaccttc accgcgatcg gtaagaattc ctccgccagc attagcgccg gatggcgtgc
     1261 ggctgatgcg taaaacacga tttcctttca tccactcttt tgtcagcaga cctcgatcgg
     1321 tatagtcagc gtccaggtat gcttcgaaaa aagcagtcat taaccccaga ctggaattaa
     1381 cgggattagg gaaaactttg tctgtgtcgc gtaccagttt gtgaaggtcg cgaatctcca
     1441 gcgggtcgag cagctttgtg ttgtgggaaa tagccagggc agtaacagca ggcagttctt
     1501 cagctcgtgc gatatgtaat gcctgaagtt cttcccgtga aacgtgcgtt accggttttt
     1561 cgctgccatg ttgcgcaagc cagcgaatgg gcagttcctg accggaaatc ggcaggagca
     1621 tattatcctc aatctcagcc atgtcttcgc cattgacgtt ggtattgtca gagctggctg
     1681 gtttgtcctg aactgagggg gaaggcgcta taaatatcat tgtgatgcca tcttccccgc
     1741 ctttttcata gcggttgcag aattcagtat cgaacacacc ttcaggcggc aggtcattaa
     1801 caatgggcaa attcacgcga acaggttttt tgaaatcctc ttcgtcaaat ccagcatcgt
     1861 ccattgcgac aacaccccgt gatattgcaa ccgataattt ttttgctgtg cgccagtaaa
     1921 aaccgccttt aatcccaagg cgttttctga ctttgtcatt tttggcttcg taatacagtg
     1981 caatttcttc tttatcagtg ctcattgata aacctcataa ctattttaag gttgtacgaa
     2041 tccctgccat tgctggcatg cttaatcaac gggtatggcg ttaatacggc tggcgggtta
     2101 tccagccggt atttcgttat tcaggtacag cgatactttg tttaacggga gacattcacc
     2161 ggaaattttt tgctcgtctc ttgcctgatg gcaggattct ttactggcat aaattccggt
     2221 aatcacattc tgtggctcac ctgttataag aaaaaccgtc atcatcagtg caaacgctga
     2281 actcactgct gccctccgaa aatgccaagt tcaagaaggg caattctgga gagtatggaa
     2341 ttatcattga gaagataagg ctcatatttt ctcatcctga tggcgtcttc agtaaactcc
     2401 cggttactga gcagaatacc aatattaaaa cacccttcag acgtattaac gtttggtagt
     2461 gacgtttcca ttatcgcgtc ctcaacaatg aattttgtga tgtggtgcct ggtgcctcca
     2521 ggtgacgtta accagttaac aattaacgcc ggaatacaga atcacccata acgctgtttt
     2581 tagctttaac tgttccgcgt gcgctcagcc gcattcacca catcacaaaa ttcactttaa
     2641 aaagggcggc agagcagtca cggagtaaaa ctgataccgc caaacgtcac cagaaaattg
     2701 ataacagaag gcgttgcagc ggggttgtca cttaagcgta tggtcaacct gacaacccgg
     2761 tgtcctcaac ggggaagaaa taaccccgcc atacttaccg ccgcaccatt tcgcgggttg
     2821 ccacaaccgg aagcgcacgg tcgacgaaaa tttaacgaca ggctatctat gaaccagcta
     2881 cctcgccgtg cgctttcgcg ttatggtctg acttttcagg gaaatatcct ttcagtaaac
     2941 tgtcagtgcc ggatgctcac ccgcgtccgg cgcacgcact ccacctcacc cgtggagaac
     3001 tacttaatta ccaaccttag cttcgttggt tagctattaa cgcgggtatg taatcattct
     3061 ggcaatgctt aatgccgctg ctttttccag attggtgata tcctgctcca gagcggacag
     3121 tttttcagcc tgcttagccc tggcttcatt ggcccatttc agatcctgcg ctgcattaat
     3181 tttctggcgc atccactcat aaagttcatc atcggtatag tctggtgcga tgatgacggg
     3241 ttctcgtttc tgcatactga ttcctcgcgg tgctgtttcg cttatcagcc gttagatttt
     3301 gccgagctgg aaagcgcctg tttaaattcg ttgaagctga gagcttcttc gccgtcggca
     3361 agaccttcga agtattcttc ataagccttt tccatgattg tgtcgaaatc catatcactc
     3421 acctgagttt ctttccagcc agcgacgggc accattttcg gttttaaacg ttttgctttt
     3481 ggtatacgtc atcgcggtga acgtgccgtc ctggttgggg aacacgccac ataccagaga
     3541 ttcgctgttg ccaagatcga tagtatccat gctgacctca tttcccctta acgccggggt
     3601 agcggaactg tttgctgaga acaccgtgcg gtgtcttgat gcaagcaaga ttagccatga
     3661 ctaacagttt ggtcaagtat ttttgtttgt catggctaac attttgggca accaaaaaga
     3721 taacgcattg attgcgttat cttttgtttg ttcgttgacg ggcttttaat aactcttcaa
     3781 agagtttgtt gaaatttttt actcgggcgc gcatctcggt gagctgagca tcctgttctg
     3841 attcaggcaa tgcattaaaa agctcaagga gctcatgttc tttgggggat agagcaactg
     3901 gctcctcaat aggtggtgat ggctgcttgt cttcatcgcc aaatagaatc catgttggcg
     3961 agcactgcag tactttgctg agggcaaaaa gattctttcc tgtaggttcg ctatcatccc
     4021 gctcccattg tgaaaccgat acgtgagaaa ttttcaggac tttagcaaga gacctttggg
     4081 tgtatttgag gttttttcgg cgatatctaa tgcgttcgcc aatggttaaa ttttttgtat
     4141 ccatagttag ctaatgctaa atcttattga ctatgttttt gttaacattt attttgttag
     4201 tcatggctaa caattaaggt gctttaaatg cttaaaactg acgcactttt gtatttcggt
     4261 tcaaaaacaa aacttgcaca agctgctggt attcgtttgg cttcgcttta tagctggaaa
     4321 ggggagctag tacctgaagg tcgcgcgatg cgcctgcaag aggcatccgg cggggaactt
     4381 cagtacgacc ccaaagttta tgacgaatat cgtaaggcaa agcgggcggg gcggttgaac
     4441 aatgaaaatc acccctgaac aggtttgtga ggctctggat gcctgggtgt gccgaccagg
     4501 aatgacacag gaacaggcga cgatattaat cacggaagca ttctgggctc tgaaagaacg
     4561 cccgaacatc gatgttcaac gcatcacgtt taatgatggc gaggttgatc aacgggcgct
     4621 gggcgttaac cgggtgaaga tattcgaacg ctggaaagct atcgacacca gagataagcg
     4681 tgacaaattc acggcgctga ttccggcaat tatggaggct atccggatca gcgatttcag
     4741 attgtattgt gaaattactg acggaaaaag cattacgtac atgatcgccg ggttaaacaa
     4801 agaatatggc gatgtggtgg agtccgggct gctttttgcg gatccagttg ttgtggaacg
     4861 tgagactgac gagcttatag aaaaagctat tgctttcaag cacgcgtatc gtcagcaata
     4921 ccaatattac tttgcagata aacaaatgtc tgccaggggt tcgtatgagt atcgatgcac
     4981 tacgatgggc taaaaaggtg aaaaccggca gttcatccag taagtcagta ttgacctggc
     5041 ttgctgatat gtgcggtgcc gatttgtgtg catacccgtc tgtatctgca ctggcagaag
     5101 taacggaact gaacaaaaag actgtgcagg acagcttacg acacctgatg gagattgggt
     5161 taattgttga taccggtgag agaaaaggca gaacaaagca aattgtggtg taccgactta
     5221 tcggtgtaga agaaagtgtt gccgagcctg aatacaccca aaaacgggtg tctttaaagg
     5281 tgggtaaaat tggtgctgtt aataaaaaca gtaccgaaaa cggttatgtt tcagcacaaa
     5341 agagccccaa aaacggaact ctttgttgca tggaaaataa ccaaagacac ccaaattttc
     5401 catcaaagac acccaaaaac ggatcacgga acccaaagga acccaaagat ctaaacccca
     5461 cacataacgc acgcgagagt gctccgacca gtgagcagga agttttgtcg ttacaggcag
     5521 ccccccatgt attcctggat ggcctgagcg aacccatcgg aaaatttccg atgaccgata
     5581 gctggtatcc gtcacgggat tttcgacgac gggctgcgtt gtgggggatg gctttgccgg
     5641 agacagaatt tacacctgct gaacttaccg cattccggga ctactgggca gcggagggga
     5701 aagtgtttac gcagattcag tgggagcaga aattcgcccg tcacgtaaat cacgtcaggg
     5761 cgcaggttaa accagtcagc aagggggtaa accatgcagc agcaccaggt ggcaccgcat
     5821 cacgggcagt tcaggaaatt cgggcagcac gtgagcagtg ggaacgtgaa aacggattta
     5881 tcagcgacgg aaacggcctg gaagctgtgg gaactcatgg gggaggttta ttcgaaccgc
     5941 tggacccaga agaacggggc cgcaccttcg aagctctgga ttgcacagat tggcgcgatg
     6001 actgagcagc aaatccggca ggtctgccgc cagtgcatgg accgctgccg ggcgggtgaa
     6061 acatggcctc cggacctggc tgagtttgtg gcactgattt cgaaaagcgg agccaatcca
     6121 ttcggtctga cggtggatgc tgtgatggag gagtaccgcc gctggcgcaa tgagtcctgg
     6181 cgatacgacg gaagtgataa gtacccgtgg tctcagcctg tgctgtatca catttgcctc
     6241 gagatgcgtt caaaggggat tgagcgccag atgaccgaag gggaattaaa acggcttgca
     6301 gaacggcaac tgacgaaatg ggcaaagcat gttagtaacg gcctgagcgt tccgccagta
     6361 cggcgacaac tggcggcacc aaaacgcccg ttgggaccaa cgccaattga gttgctgaaa
     6421 caggagtatg aacgccggaa agcggctggt tttgtctgat ttgagaagta atttttatcc
     6481 ggaggaaatt ttaatgggaa ccgtattgca tgcactgaaa gcgatgggta aagccaattc
     6541 tgttgaactg gcggcgcggc ttgatatcag ccgtgaagaa gttctcaacg aactgtggga
     6601 actcaaaaaa aatggcgttg ttgataaaac gggtcacacc tggtttctgg ctggtgaagg
     6661 tgaatccggg gtaaccgaag aacagccagc acagtctgaa gtaccggatg tgctgaccgg
     6721 ggaggtcgaa caaaaagtta ctgcggacat gatgattgag tttatctgtc aggatggggc
     6781 taaaacgtgt gaggaactgg cggataagtt cggtgttagc attcgcaagg ttgcttccac
     6841 gttggcggtc gtaacagcaa cggggagcct ggcacgcgta aatcagaacg gtaaatttcg
     6901 ttactgcata ccgggcgctg atttaccggc agagccggaa gctgcatcgg taacggaaac
     6961 cgatggtaaa gcctttcctc agccaacagg tgttgcgtta ccagtccggg aagcggaaac
     7021 acaggaagaa ataaaaactg aaagtgtggc ggtcacagtg cagtcacagc cgttgttcac
     7081 cagaaaacat ccggatggtc tgattttacc atcgctgcat gtggctaacc gcgagctgcg
     7141 ccgggcaaaa ggtcaggttc agaagtggga gcgtgtctgc gccgcgctgc gggagctgaa
     7201 caagcaccgg gatattgttc ggcagattgt cgattcatcc ggtcgtattg tgtcggaaaa
     7261 gtgattgccg gaggcgctta tggcgaaacc ttttacacac gaacagcgtg aagaactgaa
     7321 ggcccgaatt atcgggctgg tacgcaaaaa tgaacgcatg acgatatcac aactggagag
     7381 agcgacggga gcaggctggc attcagtcag acgttgcctt gtggatgtac tggcttgcgg
     7441 cgatttatac atgcccggta aatacggtgt ttttacatca gaacaggtgt atcgcgtatg
     7501 gcgtaaggca gcggagaaaa caaccgacca gacattgatt cgaaagttac cagacggaga
     7561 aatacgccgc tacgacagac aacagaacat aatctgtggc gagtgccgga agagtgaagt
     7621 tatgctgcgt gtactggcgt tctatcaggg caattttcag gaggcggtat tgtgagtgaa
     7681 ttagctatca ggcttcaatt gtcgctggca ttcacatcaa aggagaagag catggaaata
     7741 aaaccagaag atgagttaag caatatcgtt ttatttccgg taaaagagga tgacccacgt
     7801 aatcaggtta attttcttta tgagccatcg gaaagagcat attgtcatca cgcctctgtt
     7861 cgggttgacg aaaaagagcg tcaggtccgc tgtaaaatct gcggtgcagt tgtggagcca
     7921 tttgactgga tgctctctgt ggcgaaaaga gaaaccagac tggcagatga tgtaaggatc
     7981 ttgcgccagg aggaacggga aaggcggaga aatatagaaa agctaattca gattgagcgt
     8041 aacgtgaaag cgcggatacg cagggtgaca aaatccagaa ctgaataatt aaatttagct
     8101 ctgttaaaaa tttaatcctt aaccggaggt atatcaatgt caaacgcaca gaaagttatt
     8161 aacgctgaaa aatataacga gtgggtgaaa aagttctcag agcagatttt taaaattact
     8221 ggcgacgaga atgcggcaaa aaatgaatta gagccgtgga cacctgaagg tgtcgaccca
     8281 aattattgct ggtgggatgt tgatccagtt gatgctgcaa atgaagctat gagttatcac
     8341 aacgattaat gtcaggaggc cgcccgaaag ggcggtaatg aaaagtgact gaattaacca
     8401 aagagaaatt aatcgaagaa gccaaattaa aaatagcgat tgcgaaatgc taccccaatt
     8461 cagggatggc acgggtagag ggcgagttat tcaaaattgc actggcatcg ctggaagcag
     8521 aaccgatagc gtgggaatgc ggtgaaaaca taatcctgtt taaccctgac acagttgaag
     8581 catacgcaaa acgtgcggaa atatcaccta aaccactatt ctccgcgccg ccagcgctgg
     8641 tagtgcctga taagttgccg cgtgaataca gaaacggttg gcctcttgcg tatagtgatt
     8701 atgctgaagg ctggaacgac tgccgcgaag ccatgcttca gggagataaa tcatgattaa
     8761 tcgtatcaag ctggagcaca tcctcgaata tgccaggcag cagaggcata ttggtcagca
     8821 ttgtaaaatt ccaccaggag atatggttga aatcatggag attgccatgc gcaaggctgg
     8881 caattctccg gtaactccgg atggttggat aagctgtagt gagcgaatgc cgaataccaa
     8941 aacagccgtt cttgttgccg tggagtttga caggaaaggt gactggcgaa tgaaattggc
     9001 tacttacatc ccggggcatc ctgacgctaa tgatgggtgg ataattcctg gtgcgtcgtg
     9061 gaaaccgtca cactggatgc cgctaccaga accgccgcag gaggtgcgcc aatgatctgg
     9121 cctgaagcct ttgcaattac aggcgttgct atagctattg attttttagt atatgttatt
     9181 tgtcggtggg ggtaaaaacg ttcgccggga tt
//
LOCUS       DAZKHC020000146         9155 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZKHC020000146 DAZKHC020000000
VERSION     DAZKHC020000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.146"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..555
                     /gene="lpfB-O113"
                     /locus_tag="ACHOSM_RS21490"
                     /old_locus_tag="pgaptmp_004298"
     CDS             <1..555
                     /gene="lpfB-O113"
                     /locus_tag="ACHOSM_RS21490"
                     /old_locus_tag="pgaptmp_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HGU4560666.1"
                     /translation="FSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKI
                     LRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLI
                     PEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANS
                     AGGQMTWRFINDYGAVSDPIKMTL"
     gene            580..3102
                     /gene="lpfC-O113"
                     /locus_tag="ACHOSM_RS21495"
                     /old_locus_tag="pgaptmp_004299"
     CDS             580..3102
                     /gene="lpfC-O113"
                     /locus_tag="ACHOSM_RS21495"
                     /old_locus_tag="pgaptmp_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HGU4560667.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3113..4186
                     /gene="lpfD-O113"
                     /locus_tag="ACHOSM_RS21500"
                     /old_locus_tag="pgaptmp_004300"
     CDS             3113..4186
                     /gene="lpfD-O113"
                     /locus_tag="ACHOSM_RS21500"
                     /old_locus_tag="pgaptmp_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HGU4560668.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            4434..5474
                     /gene="pstS"
                     /locus_tag="ACHOSM_RS21505"
                     /old_locus_tag="pgaptmp_004301"
     CDS             4434..5474
                     /gene="pstS"
                     /locus_tag="ACHOSM_RS21505"
                     /old_locus_tag="pgaptmp_004301"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGU4560669.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANATKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            5561..6520
                     /gene="pstC"
                     /locus_tag="ACHOSM_RS21510"
                     /old_locus_tag="pgaptmp_004302"
     CDS             5561..6520
                     /gene="pstC"
                     /locus_tag="ACHOSM_RS21510"
                     /old_locus_tag="pgaptmp_004302"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HGU4560670.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            6520..7410
                     /gene="pstA"
                     /locus_tag="ACHOSM_RS21515"
                     /old_locus_tag="pgaptmp_004303"
     CDS             6520..7410
                     /gene="pstA"
                     /locus_tag="ACHOSM_RS21515"
                     /old_locus_tag="pgaptmp_004303"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGU4560671.1"
                     /translation="MAMVEIQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            7501..8274
                     /gene="pstB"
                     /locus_tag="ACHOSM_RS21520"
                     /old_locus_tag="pgaptmp_004304"
     CDS             7501..8274
                     /gene="pstB"
                     /locus_tag="ACHOSM_RS21520"
                     /old_locus_tag="pgaptmp_004304"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGU4560672.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            8289..9014
                     /gene="phoU"
                     /locus_tag="ACHOSM_RS21525"
                     /old_locus_tag="pgaptmp_004305"
     CDS             8289..9014
                     /gene="phoU"
                     /locus_tag="ACHOSM_RS21525"
                     /old_locus_tag="pgaptmp_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGU4560673.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         2354 a         2117 c         2331 g         2353 t
ORIGIN      
        1 tttagcaaag aagaaaaaat accttatctc atccaggctt gggtagatcc atttaataaa
       61 gaggataaaa gcaaagcgcc gtttaccgtt attccgccag tatcacgatt agaaccttcg
      121 caagaaaaaa tattacgcat tattcacacc aaaggtgtgt cattgcctga tgatcgcgag
      181 tcagtcttct ggctgaatat taaaaatatt ccgccatcag cgagcaacaa agcgacgaac
      241 tcgctggaaa tagcagtaaa aacgcgtatc aagctgttct ggcgaccagc aaatatccgc
      301 cttattcccg aagatgctgc gccgaaagtg aaatggcgcc gggaagggcg aaatctgata
      361 gcagagaatc ccaacccaat tcatatcagt gtgatggatg tcatcgttga tggccatgat
      421 gtgcccctca atatgatccg tccttttgaa acgctcacgt tacctttacc tgctaattct
      481 gcgggaggcc aaatgacctg gcgatttatt aatgattacg gcgcagtcag tgacccgata
      541 aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac cagaatagtg
      601 gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc cgaggtcagt
      661 gtattcaatc cggcgcttct ggaaatcgac catcaatccg gagtcgatat tcgccagttt
      721 aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat caacggtaaa
      781 atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga tctgcacgct
      841 tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga tgcgctcaaa
      901 tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat tgaaggctca
      961 tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcccca aatctacatg
     1021 gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat taacgcgctc
     1081 accatcaact acgatttttc tggttcacat acactacgtt cagattatgg ttcacaagag
     1141 acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg gcggctacgt
     1201 aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat tagtacctgg
     1261 atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac gtggacggcg
     1321 agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga taacgatatg
     1381 ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa gtccaacgcc
     1441 accgtcatca ttcggcagaa tggctacgtg atttatcagt ctgccgttcc acaaggtgct
     1501 tttgagatca ccgatctcaa cactgccagt acaggtggcg atttggacgt aaccatcaaa
     1561 gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc gattcttaaa
     1621 cgtgaaggtc agacagatgt tgatgtcagc gtgggtgaat tgcgcgatga agacggattt
     1681 acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat cactttatat
     1741 ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg taaagatctt
     1801 ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt tagccatgat
     1861 gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aacgatttga cgacacagac
     1921 actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac cctcaatgag
     1981 tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg acgtagtcgc
     2041 gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata cctgacatta
     2101 agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt tggctacagc
     2161 agtagctgga agcgtctctc gtggaacgtc tcctggagtt attccaatac tgccagacag
     2221 gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat gctctcttta
     2281 tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt ttcgcaaaac
     2341 gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga aagaaataac
     2401 ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg cggtaatatg
     2461 tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta cagccaaaat
     2521 tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga aggggtaacg
     2581 ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc cgccgggtta
     2641 gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt caagacacag
     2701 ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc gaagtcgaat
     2761 atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt ggttaaagcg
     2821 gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc aaatggtaaa
     2881 ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga ttcaggaatt
     2941 gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca ggttacgtta
     3001 tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcactttc gacaccagaa
     3061 agtgagagcg gattaattga acagggtgtg acatgtcatt aaaaggattt ttatgaacaa
     3121 gtatatcaaa cagtggtgct ttgctgtgtt tatgctctcg ttaagtagtg tagcacttgc
     3181 agctcctaaa ggtatctgta cccctgataa tggcgtattt cacagtacgc ttgatttctc
     3241 gggatatctt attaccgcga acgaaaataa ggttggaacg acatttaaca ccactgtaac
     3301 taatggatcc tcatatcctg gacgctgtca ttgtgatact ggtaacgtag gagaattccc
     3361 ctatatctat tatacgtcaa aaataaacca ggcattaact tatgctgggg ttcactctaa
     3421 tattaattat tatgatctta atccgaacct tgatgttggg attgctatag atattcttgg
     3481 ggttgggtat gtgaatgcac cttttgaata tcatgctaac aacccgtctg gtaatacaaa
     3541 gtacaattgc aaccgcattg aacctttaag tatatctagt ggggctaaag cgatagtata
     3601 tttttatatc aagaagacat ttgcaggaaa attgattatt cctgaaacaa agatagtgac
     3661 attgtatgga acaattagcc gtgacacccc ggtggattac tcacagccga tggctgatgt
     3721 ttatattcgg ggcgatatta cagctccgca aagttgcgaa ataaataatt tacagccagt
     3781 ttattttgat tttaaagaaa taccagctgc agatttttca tctgttgttg gaagtgcggt
     3841 aacaacacat aaaattacta aaacggtcac tattgagtgt gaaaacctgg ggatactgaa
     3901 taccgatgat atcagtacgt ctttttatgc taccgaaccc aatacggaca attcaatggt
     3961 cgtaacttca aactctaacg tggggataaa aatttacgac aaaaataata aggaaatcaa
     4021 agtgaacggt ggtgagttgc caacagacat gggtaaatca acagtgtatg gtgaaaaatc
     4081 aggtagtgta actttttcag ctgctcctgc gagccttact ggagctcgcc cagccccggg
     4141 gcaatttact gcaacagcga cgataactgt tgagattgtg cgctaatagg tctgacaacg
     4201 attgttcatc acatcgttgt catctcaccc atttctttat cacttttaaa tgcatcatga
     4261 caaaaagatg acatgtggga ttttcatctt cacatttaaa atcagtaagt tatttggtaa
     4321 ttcctttctg tcataaaaat gtcatattcc ttacatataa ctgtcacctg tttgtcctat
     4381 tttgcttctc gtagccaaca aacaatgctt tatgaatcct cccaggagac attatgaaag
     4441 ttatgcgtac caccgtcgca actgttgtcg ccgcgacctt atcgatgagc gctttctctg
     4501 tgtttgcaga agcaagcctg acaggtgcag gtgcaacctt ccctgcgccg gtgtatgcca
     4561 aatgggctga cacttaccag aaagaaaccg gtaataaagt taactaccag ggtatcggtt
     4621 cttccggtgg cgtaaaacag attatcgcta ataccgttga ttttggtgcc tctgacgcgc
     4681 cgctgtctga cgaaaaactg gctcaggaag gtctgttcca gttcccgacc gtgattggcg
     4741 gcgtggtgct ggcggttaac attccagggc tgaagtctgg cgaactggtg ctggatggta
     4801 aaaccctcgg cgacatctac ctgggcaaaa tcaagaagtg ggatgatgaa gccatcgcca
     4861 aactgaatcc gggtctgaaa ctgccttcac aaaacattgc tgtagtacgc cgcgcagatg
     4921 gctccgggac ttccttcgtc ttcaccagct acctggcgaa agtgaacgaa gagtggaaaa
     4981 acaacgttgg tactggctct accgtaaaat ggccgatcgg tctgggcggt aaaggtaacg
     5041 acggtatcgc cgcgttcgtt cagcgtctgc cgggtgcaat tggttatgtt gaatatgctt
     5101 acgcgaagca gaacaacctg gcgtacacca aactgatctc cgctgatggt aaaccggtta
     5161 gtccgaccga agaaaacttc gctaatgcaa caaaaggtgc agactggagc aaaaccttcg
     5221 ctcaggatct gaccaaccag aaaggcgaag atgcatggcc tattacctct accacgttca
     5281 ttctgatcca caaagatcag aagaaaccag aacaaggcac agaagtgctg aaattcttcg
     5341 actgggcgta caaaaccggg gctaaacagg cgaacgacct agattacgcc agcctgccgg
     5401 atagtgtagt tgaacaggtt cgcgctgcgt ggaagaccaa tattaaagac agtagcggta
     5461 agccgctgta ctaataaaac tccaggccgg gtacggtgtt ttacgccgca tccggcatta
     5521 caaaatgact ttgtaaacgc gtttaactga agagtaactt atggctgcaa ccaagcctgc
     5581 ttttaaccca ccgggtaaaa agggcgacat aattttcagc gtgctggtaa aactggcggc
     5641 gctgattgtg ctattgatgt tgggtggcat tattgtctct ctgatcatct cctcctggcc
     5701 gagcattcag aaatttggtc tggctttcct atggaccaaa gagtgggatg caccgaacga
     5761 tatctacggg gcgctggtgc cgatctacgg tacgttggtg acttcgttta tcgcgctgct
     5821 gatcgccgtc ccggtgagtt tcggtatcgc cctgttcctg actgagcttg cgcctggctg
     5881 gctgaaacgc ccgctgggta tcgccattga gctgctggca gccattccaa gtatcgttta
     5941 cggcatgtgg ggcctgttta tctttgcgcc gctgttcgcc gtttactttc aggagccggt
     6001 cggcaatatc atgtcgaata tcccgattgt tggcgcgctg ttctctggcc ccgcatttgg
     6061 tatcggtatc ctcgcggcag gcgtgatcct cgccatcatg attattccgt acattgcggc
     6121 ggtaatgcgt gatgtgttcg aacaaacccc ggtgatgatg aaagagtcgg cctacggtat
     6181 tggctgcacc acctgggaag ttatctggcg tatcgttctt ccgttcacca aaaatggtgt
     6241 tatcggcggc atcatgctgg ggctgggccg cgcgctcggt gaaaccatgg cggtgacctt
     6301 tatcatcggt aacacctacc agctcgacag cgcctcgctg tacatgccgg gcaatagtat
     6361 cacctctgcg ttggcgaacg aatttgcgga agcggaatcc ggtctgcacg ttgccgcact
     6421 gatggaactg ggcctgatcc tgtttgtgat taccttcatc gtcctcgccg catcgaagtt
     6481 tatgattatg cgcctggcta agaatgaggg ggcacgctaa tggctatggt tgaaatccaa
     6541 accactgcgg cgctggctga atctcgccgc aaaatgcagg cgcgtcgccg cctcaaaaac
     6601 cgtattgcgc tgacgctctc gatggcgacg atggccttcg gcctgttctg gctgatctgg
     6661 attttaatgt ccaccatcac tcgcggtatc gacggtatgt cgctggcgct gttcactgaa
     6721 atgacgccgc cgcccaatac ggaaggtggt ggtctggcga acgccctggc gggtagcggg
     6781 ctgttaattt tgtgggccac ggtattcggt acgccgctgg gcattatggc ggggatttat
     6841 ctggcggaat atggtcgtaa atcctggctg gcagaagtga ttcgcttcat taacgacatt
     6901 ctgctctccg cgccgtcgat tgtggttggt ctgtttgttt acaccatcgt ggtggcgcag
     6961 atggagcact tctctggctg ggcgggtgtg attgccctgg cgctgttgca ggtgccgatt
     7021 gttatccgca ccaccgagaa catgctgaaa ctggtgccgg acagcctgcg tgaagcggct
     7081 tatgcgctgg gtacaccgaa gtggaagatg atctccgcga ttacgctgaa agcgtcggtg
     7141 tccgggatca tgaccggtat cttgctggcg attgcccgta ttgctggtga aaccgcgccg
     7201 ctgctgttta ccgcgctctc caaccagttc tggagcacgg acatgatgca gccgatcgcc
     7261 aacctgccgg tgacgatctt taagtttgcg atgagtccgt ttgcggaatg gcagcaactg
     7321 gcctgggccg gggtattgat cattaccctg tgcgtactgc tgctgaacat tctggcgcgc
     7381 gttgtttttg cgaagaataa acacggttaa cactttgccg gatgcggcgt gaacgcctga
     7441 tccggcctac ggtaagcctg attagcgaag tgcatcaggc acgatgagga aaagattgca
     7501 atgagtatgg ttgaaactgc cccgagtaaa attcaggttc gtaatttgaa cttctactac
     7561 ggcaaattcc atgccctgaa aaacatcaac ctggatatcg ctaaaaacca ggtaacggcg
     7621 tttatcgggc cgtccggctg cggtaaatcg acgctgctgc gtaccttcaa caaaatgttt
     7681 gaactgtatc cggagcagcg tgcggaaggt gaaattctgc ttgatggcga caacatcctg
     7741 accaactctc aggatatcgc cctgctgcgt gcgaaagtgg gcatggtgtt ccagaaaccg
     7801 acgccgtttc cgatgtccat ctacgacaac atcgcttttg gcgttcgtct gtttgagaag
     7861 ctctcccgtg ccgacatgga cgagcgcgtg cagtgggcat tgaccaaagc cgcattgtgg
     7921 aacgaaacca aagataaatt gcaccagagc ggttactctc tctctggtgg tcagcaacag
     7981 cgtctgtgta ttgcgcgtgg tatcgccatt cgcccggaag tgctgctgct cgacgaaccg
     8041 tgttcggcgc tcgaccctat ctctaccggg cgtattgaag agctgatcac cgaactgaag
     8101 caggattaca ccgtggtgat cgtcacccac aacatgcagc aggctgcgcg ttgttccgac
     8161 cacacggcgt ttatgtacct gggcgaattg attgagttca gcaacacgga cgatctgttc
     8221 accaagccag cgaagaaaca aacagaagac tacatcaccg gtcgttacgg ttgattcagg
     8281 agtgcgttat ggacagtctc aatcttaata aacatatttc cggccagttc aacgccgaac
     8341 tggaaagtat ccgcacgcag gtgatgacca tgggcggcat ggtggagcag cagctttctg
     8401 atgcaatcac cgcgatgcat aaccaggaca gcgatctggc gaagcgcgtg atcgaaggcg
     8461 acaagaacgt caacatgatg gaagtggcga tcgatgaagc ctgcgtgcgc attatcgcca
     8521 aacgtcagcc gacggcgagc gacctgcgac tggttatggt gatcagtaaa accattgccg
     8581 agctggagcg tattggcgac gtggcggaca aaatctgccg tactgcgctg gagaaattct
     8641 cccagcagca tcagccgttg ctggtaagtc tggagtcgct gggccgtcat accatccaga
     8701 tgctgcacga cgtgctggac gcgttcgcgc ggatggacat tgacgaagcg gtacgtattt
     8761 atcgtgaaga taaaaaagtc gatcaggaat acgaaggtat tgttcgtcaa ctgatgacct
     8821 acatgatgga agattcgcgt accattccga gcgtacttac tgcgctgttc tgcgcgcgtt
     8881 ctatcgaacg tattggcgac cgctgccaga atatttgtga gtttatcttc tactacgtga
     8941 aggggcagga tttccgtcac gtcggtggcg atgagctgga taaactgctg gcgggaaaag
     9001 atagcgacaa ataattcacc agacaaatcc caataactta attattggga tttgttatat
     9061 ataactttat aaattcctaa aattgcacaa agttaataac tgcgagcatg gtcatatttt
     9121 tatcaatagc gcattgctat tttctctgca cgcaa
//
LOCUS       DAZKHC020000147         8913 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZKHC020000147 DAZKHC020000000
VERSION     DAZKHC020000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.147"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            9..1367
                     /gene="dgcT"
                     /locus_tag="ACHOSM_RS21530"
                     /old_locus_tag="pgaptmp_004306"
     CDS             9..1367
                     /gene="dgcT"
                     /locus_tag="ACHOSM_RS21530"
                     /old_locus_tag="pgaptmp_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGU4560674.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GAIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
     gene            1371..1712
                     /locus_tag="ACHOSM_RS21535"
                     /old_locus_tag="pgaptmp_004307"
                     /pseudo
     CDS             1371..1712
                     /locus_tag="ACHOSM_RS21535"
                     /old_locus_tag="pgaptmp_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            1709..2695
                     /gene="ycdU"
                     /locus_tag="ACHOSM_RS21540"
                     /old_locus_tag="pgaptmp_004308"
     CDS             1709..2695
                     /gene="ycdU"
                     /locus_tag="ACHOSM_RS21540"
                     /old_locus_tag="pgaptmp_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HGU4560675.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGLWLTFKSIIALLILCGVVLAINLF
                     FLCFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(3070..3157)
                     /locus_tag="ACHOSM_RS21545"
                     /old_locus_tag="pgaptmp_004309"
     tRNA            complement(3070..3157)
                     /locus_tag="ACHOSM_RS21545"
                     /old_locus_tag="pgaptmp_004309"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3121..3123),aa:Ser,seq:gga)
     gene            3391..4329
                     /gene="ghrA"
                     /locus_tag="ACHOSM_RS21550"
                     /old_locus_tag="pgaptmp_004310"
     CDS             3391..4329
                     /gene="ghrA"
                     /locus_tag="ACHOSM_RS21550"
                     /old_locus_tag="pgaptmp_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HGU4560676.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            4384..5121
                     /gene="ycdX"
                     /locus_tag="ACHOSM_RS21555"
                     /old_locus_tag="pgaptmp_004311"
     CDS             4384..5121
                     /gene="ycdX"
                     /locus_tag="ACHOSM_RS21555"
                     /old_locus_tag="pgaptmp_004311"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HGU4560677.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            5145..5699
                     /gene="ycdY"
                     /locus_tag="ACHOSM_RS21560"
                     /old_locus_tag="pgaptmp_004312"
     CDS             5145..5699
                     /gene="ycdY"
                     /locus_tag="ACHOSM_RS21560"
                     /old_locus_tag="pgaptmp_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HGU4560678.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            5801..6292
                     /gene="ycdZ"
                     /locus_tag="ACHOSM_RS21565"
                     /old_locus_tag="pgaptmp_004313"
     CDS             5801..6292
                     /gene="ycdZ"
                     /locus_tag="ACHOSM_RS21565"
                     /old_locus_tag="pgaptmp_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGU4560679.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(6356..7189)
                     /gene="csgG"
                     /locus_tag="ACHOSM_RS21570"
                     /old_locus_tag="pgaptmp_004314"
     CDS             complement(6356..7189)
                     /gene="csgG"
                     /locus_tag="ACHOSM_RS21570"
                     /old_locus_tag="pgaptmp_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HGU4560680.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(7216..7632)
                     /gene="csgF"
                     /locus_tag="ACHOSM_RS21575"
                     /old_locus_tag="pgaptmp_004315"
     CDS             complement(7216..7632)
                     /gene="csgF"
                     /locus_tag="ACHOSM_RS21575"
                     /old_locus_tag="pgaptmp_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HGU4560681.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(7657..8046)
                     /gene="csgE"
                     /locus_tag="ACHOSM_RS21580"
                     /old_locus_tag="pgaptmp_004316"
     CDS             complement(7657..8046)
                     /gene="csgE"
                     /locus_tag="ACHOSM_RS21580"
                     /old_locus_tag="pgaptmp_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HGU4560682.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(8051..8701)
                     /gene="csgD"
                     /locus_tag="ACHOSM_RS21585"
                     /old_locus_tag="pgaptmp_004317"
     CDS             complement(8051..8701)
                     /gene="csgD"
                     /locus_tag="ACHOSM_RS21585"
                     /old_locus_tag="pgaptmp_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HGU4560683.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYSMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         2265 a         1898 c         2148 g         2602 t
ORIGIN      
        1 gtagctttat ggaaaaagac tatttgagaa ttagtagtac tgtattagtg agcttattgt
       61 ttgggcttgc tttggttttg gtgaatagtt ggtttaatca gccaggcgtt gaggaagttg
      121 ttccacgatc aacctatctg atggtaatga ttgctttatt ttttatcgat actgttgcat
      181 ttatttttat gcagttgtat ttcatttatg accgtaggca attttcaaac tgtatactta
      241 gtttggcttt tctgagttgt ttgatttact ttgttaaaac cgtcattatc attcagcaaa
      301 ttattgaggg gcgtttgaca agcagtgttg tccaaaatga cattgcaatc tattatttgt
      361 ttcgtcagat gagtttgtgc atattaatat ttctggcatt ggtgaataaa gttagtgaaa
      421 acacaaaaca gcgcaattta ttttccaaaa aaatgacttt gtgcataagt ttgttttttg
      481 tttttggggg ggcgatagtt gctcacatac tttctagtca ttatgagagc tacaatttac
      541 acattgccga gttaaccaat gaaaatggtc aggtggtttg gaaagcctca tatgttacca
      601 taatgatttt catgtggtta accttgcttt cggttaatct atactttaat ggtttacgct
      661 atgatatctg gaatggagtg acggtgattg ctttttgtgc cgtactgtac aatatctctc
      721 tgttatttat gagcaggtat agcgtgtcca cttggtatat tagtcgcact attgaagtag
      781 tcagtaagtt aaccgttatg gtaatattta tgtgccatat attcagtgcg ctacgagtaa
      841 caaagaacat tgcacatcgc gatcccttaa ccaatatatt taacagaaat tattttttta
      901 atgaactgac agttcaatca gcatcagcca aaaaaacgcc ttattgcgtc atgattatgg
      961 atatcgacca cttcaaaaaa gtcaacgaca cctgggggca tccggttggc gatcaggtga
     1021 taaaaacagt ggtgaatatc attgggaaaa gcatacgacc agatgacctt ttagcgcgcg
     1081 tcggcggcga agagtttggc gtcttgctga cggacatcga tactgaacgt gcgaaagctt
     1141 tagcggaaag gattcgggaa aatgttgagc gtttaactgg cgataatcct gaatacgcta
     1201 taccacaaaa agtgacgatt agtattggcg ctgttgtgac tcaggaaaat gcgttaaacc
     1261 cgaatgagat ctatcgactg gctgataacg cactttacga ggcgaaagag accgggcgta
     1321 ataaggtggt tgtgagggat gtggtgaatt tttgtgagtc accataaagt gcctggcaca
     1381 cgcgtttgat ggaagacggc gttcaggcaa tgcgcgatta tcttgccgga ctggatatcg
     1441 cttcgccaga gcatcaggtt ctgatgaacg taacagcaaa aagcgaggtc gctccgtcaa
     1501 ttatcaaaga aaatctctca ttacacttaa ctcatacggt gaaatggact gaatctctcg
     1561 atacatttct gaatatgcca accccggtag ctttccttga aataagtaat aagccctatt
     1621 ttgggaatat gttaaatgat tttgctggcg tggatcagca acgagttatg cattgtcgta
     1681 aagcatttag tgatgcaaag gtattcaaat gattcctgat tatttaactt ttattcgctt
     1741 tcaggataaa cgaaatctga tatacattta tgctattgga cttattctga taggctttta
     1801 ttggaagaat gcagggttta cttttccatc agaggatatt ggtgtagtta gtgggattct
     1861 ggccctggtg ctgtataatt ttatttttga tctcaaggcg tactgggctt ataaatgcgt
     1921 cacgaagaat atcgattttt cgtggtttaa gaaaaagcag aaccacaaaa tagaattatt
     1981 tcttacacaa cctctggtgg caggatttct gtcgttaatc atgttgagtg caatgagttg
     2041 ggggctatac cagcttctac cctcgttata tgcgctgttc ctgatttcgt tacttgggcc
     2101 gttggtcatc tttctgctgt ttcggatgat ccgcaccagt tatgtcaagc aggtcgctat
     2161 ttcagtagcg aaaaaagtaa aatataaaag tctgactcgc tatgtgctgc tttcggtgtg
     2221 catctcaacg gttgttaacc tgcttactat cagcccgttg cgtaacagtg attcttttgt
     2281 gacagagggg ctgtggttaa cgtttaaatc gataattgca ttgctcattc tttgtggcgt
     2341 agtgttggcg attaatctgt tttttctgtg cttctccaag cggtacgctt ttctgggcag
     2401 gctttttttg caggaaatcg atctgttttt ctccagtgaa aatgcgttgt cgaccttttt
     2461 tgccaagccg ctttggcttc ggttattcat attgctggtt attgaaatga tgtggattac
     2521 gctggtgtcg gtattggcaa cgcttgtgga atggcggatt tggtttgaag cctatttttt
     2581 actctgctat gtaccgtgct taatttacta ttttttccat tgtcgattcc tctggcataa
     2641 cgattttatg atggcatgtg acatgtattt ccgttggggg cattttaata agtgaggaag
     2701 ttacaggaag tgaccagata acgcatatat gttctctact atcttgcgca ttttgattgt
     2761 tgactgagta accagacagt tgatgtgcac aatttcccct cgccctaaca gacgtgggcg
     2821 ggggcacccg gtgtgtgcaa tatacgaaaa aaaagcccgt actttcgtac gggctcttct
     2881 ttaaatatgg cggtgagggg ggggattgac tcgcttcgct cgccctgcgg gcagcccgct
     2941 cactgcgttc acggtctgtc caactggcta tcgccagttg tcgaaccccg gtcggggctt
     3001 ctcatccccc cggtgtgtgc aatatacgaa aaaaaagccc gtactttcgt acgagctctt
     3061 ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt atacacactt
     3121 tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttgttcc tgtcttgctg
     3181 gaacgggcgc taatttaggg aaatcatgac ctgaggtcaa caaacttttt gaaaaaatcg
     3241 cgcgtttatt caaacttcaa tcaatgtgtg gttttaataa gcgaaatctg ctttttttgc
     3301 caccgaccac ggatttgtta tgctggtggc ctttgtagat cataacgata agtgcgaata
     3361 aatttcgcac aacgcttttc gggagtcagt atggatatca tcttttatca cccaacgttc
     3421 gatacccaat ggtggattga ggcactgcgc aaagctattc ctcaggcaag agtcagagca
     3481 tggaaaagcg gagataatga ctctgctgat tatgctttag tctggcatcc tcctgttgaa
     3541 atgctggcag ggcgcgatct taaagcggtg ttcgcactcg gggccggtgt tgattctatt
     3601 ttgagcaagc tacaggcaca ccctgaaatg ctgaaccctt ctgttccact ttttcgcctg
     3661 gaagataccg gtatgggcga gcaaatgcag gaatatgctg tcagtcaggt gctgcattgg
     3721 tttcgacgtt ttgacgatta tcgcatccag caaaatagtt cgcattggca accgctgcct
     3781 gaatatcatc gggaagattt taccatcggc attttgggcg caggcgtact gggcagtaaa
     3841 gttgctcaga gtctgcaaac ctggcgcttt ccgctgcgtt gctggagtcg aacccgtaaa
     3901 tcgtggcctg gcgtgcaaag ctttgccgga cgggaagaac tgtctgcatt tctgagccaa
     3961 tgtcgggtat tgattaattt gttaccgaat acccctgaaa ccgtcggcat tattaatcaa
     4021 caattactcg aaaaattacc ggatggcgcg tatctcctca acctggcgcg tggtgttcat
     4081 gttgtggaag atgacctgct cgcggcgctg gatagcggca aagttaaagg cgcaatgttg
     4141 gatgttttta atcgtgaacc cttaccgcct gaaagtccgc tctggcaaca tccacgcgtg
     4201 acgataacac cacatgtcgc cgcgattacc cgtcccgctg aagctgtaga gtacatttct
     4261 cgcaccattg cccagctcga aaaaggggag agggtctgcg ggcaagtcga ccgcgcacgc
     4321 ggctactaat aaagtatcag gattcctgct atccttggcg ggaattgaat acaggagaga
     4381 gttatgtatc ccgtcgacct tcatatgcat accgttgcca gcacacatgc atatagcaca
     4441 ttgagtgatt acattgccca ggccaaacaa aagggcatta aactttttgc gatcaccgat
     4501 catggcccgg atatggaaga tgctccgcat cactggcact tcattaacat gcgtatctgg
     4561 ccgcgagtgg ttgatggggt agggatcctg cgcggcatcg aagctaacat taaaaatgtt
     4621 gatggtgaaa ttgattgcag cggtaaaatg tttgactcgc tggatctgat tattgccggt
     4681 tttcatgagc cggtttttgc accacatgac aaagcgacca atacacaagc gatgatcgcc
     4741 actatcgcca gcggcaatgt gcatataata agccatcccg gaaatcccaa atatgaaata
     4801 gatgtgaaag ccgttgctga agcagccgcg aaacatcagg tggcgctgga aatcaataat
     4861 tcttcatttt tacactcacg taagggcagt gaagacaact gtcgtgcggt agccgcagcg
     4921 gtacgtgatg ctggaggttg ggtggcatta ggctcggatt ctcacaccgc gtttaccatg
     4981 ggggaatttg aagagtgtct taaaatcctc gacgcggtag attttccgcc agagcgcatt
     5041 ttgaatgttt ctccgcgccg cttactgaac ttccttgaat ctcgcggtat ggcaccgatt
     5101 gcggaatttg cagaccttta attactagta atggaaatat attaatgaac gagttttcta
     5161 tcctctgtcg tgtgctgggt tcgctctatt accgccaacc gcaagatcct ttactggtgc
     5221 cgctgtttac cctgattcgt gaggggaaac tggctgcgaa ctggccactg gagcaggatg
     5281 agttactgac gcgtttacag aaaagctgtg atatggcgca agtctctgcc gattacaatg
     5341 cgttgtttat cggcgatgaa tgtgccgtgc cgccatatcg tagcgcatgg gttgaggacg
     5401 caacggaagc ggaagtgcgc gcttttcttt ccgaacgagg gatgccactg gcggatacgc
     5461 cagccgatca catcggcaca ttgctgcttg cggcttcctg gctggaagat cagtcaacgg
     5521 aagatgagag cgaagcactg gaaacactgt tcagtgagta tctgttaccc tggtgtggtg
     5581 cgttcctcgg caaagtggag gcccatgcaa ccacgccttt ctggcgcacc atggcaccgc
     5641 taacccgtga tgccattagt gcaatgtggg acgagctgga agaagattct gaagagtaaa
     5701 tgtgatcatc gtcactcata actgcaacag ataatttgtt gttgcataaa atgtgtgctc
     5761 gatctcattc atggccgcgt tttctgctat catgcgcggc atgaacatac ttctctctat
     5821 tgcaatcaca acaggcattc tctccggtat ctggggatgg gtggctgttt ccctgggctt
     5881 actaagctgg gcgggcttcc tgggctgtac ggcctacttt gcctgcccgc aaggtgggct
     5941 gaaagggctg gcgatctccg ctgcaaccct gcttagtggc gtggtgtggg cgatggtcat
     6001 tatttacggt agtgcactgg caccacatct ggaaattctc ggttatgtca taaccggtat
     6061 tgtggcgttt ctgatgtgta ttcaggccaa acagctgctg ctttcatttg ttccggggac
     6121 gtttataggc gcatgcgcga cgtttgccgg gcagggtgac tggaaactgg tgttaccttc
     6181 gctggcgctt gggctggtat ttggttacgc aatgaaaaac agcggcctgt ggctggcggc
     6241 gcgtagtgca aagacggctc accgtgagca ggaaatcaaa aataaagcgt gaggggcact
     6301 cacgctttcg cttaaacagt aaaatgccgg atgataattc cggctttttt atctgtcagg
     6361 attccggtgg aaccgacata tggcggtatt tcaccagaat gtcattctgc cgttctgctt
     6421 tattttgcaa atcccacaga ccacggtcga taccatcatt aatcaggaaa atgacccctg
     6481 tttcgatagc cgacatcaga cacagcataa cgggttcgtt tgaggtgtaa cccacttccc
     6541 cttccagcaa gcgctggtag tcaataaagc ggaaaacccc ggcctgaact tcataggaaa
     6601 gtatcgtctt actggtgttc accgaggaga ggatctcacc ggtactcaca ttgacgacac
     6661 gcaggttcac ggcaatctga tcgagctggt attgcgtgtc tgcaccgatc ccaaaatatc
     6721 ttgccccaac accgcccgat ttaacgttgc tttcataacc gataatcgaa ccttcaacca
     6781 taatatttgc cgccgttaaa gattgcagcg ggattcggtt attaatggca accgtgccgt
     6841 tttcttgtgc cgcacgaata atcttgcgct cgttaagcag gttttgtaag ccctggcgct
     6901 ccagcggtat aaaccagcga gaatctttca atgccgtgac cagcattgcc gtggcgcttt
     6961 gcggaacagc agtggagaag ttacttgccg ggtagggttt aaattgcccg gtttcgtcct
     7021 gaatgttgta taccgaaaca aagattttac ccgtcggcgc tggcagatgg gtcaaatctt
     7081 tgtagctctg agcacgaggc attaatgtcg gtctggcggc ttctttaggc ggggcggtta
     7141 agcatccgct cagtaacatg acggcaacca aaagaaataa gcgctgcatg attattttcc
     7201 ttatgaagct ggggcttaaa aatcggttga gttattttgt aaacccgaaa cctggatggt
     7261 cgaggtttgt ccggttttac gatctgtcac gttcaactgc aattgaccat cgcggttggc
     7321 aatatcgaca atataatcgt tggtcaccat gcggcccggt ttaccggtat taatattcga
     7381 cagtagccca cctaaaattt gtgactggat ggcctgagta aagttatcta acgctgaggg
     7441 tgtttcaata ccaaagtcat cgttatagct cggatcttta taagagtttt gggcctgagc
     7501 gctatttaat aaaaaagcgc cattatttgg gttaccacca aagtttggat tacggaactg
     7561 gaaagtcatg gttccagccc aacttaatgg cgaaataagc atgagtagaa ctactgcatg
     7621 tttgacacgc attgcagcct ccgaacaatt ttttatttag aattcatcat gcgccaaatc
     7681 gcccgtactt aataacgcct gatttatctg gcgacgattt agtgcttctt cagtttgaat
     7741 cagtgcaaag acgacagttt tctcgaagtc tcttttcaac ggaaataaaa aagtctggaa
     7801 aataacgtcc tgattgaccg ttatagtgat ccagcttccc catcgtgcac tgggcctttc
     7861 attaatcgtt aagttacccg tatagtcact ttcccattta tcactaaagg ctcggtaaaa
     7921 atcatggcca atagatgaaa cagtatggtc agttagcaat cccgggactt ctacctcaac
     7981 ggcgtgaaga ttccctgcgg cgaacagaaa ttctgccgcc acaatccagc gtaaataacg
     8041 tttcatggct ttatcgcctg aggttatcgt ttgcccagga aaccgcttgt gtccggtttt
     8101 ttacggctat cttcttgaaa agattataaa gatgcgtttt aaccgtattt tcgctgatga
     8161 acaacgaacg agcgatctcg ttattagacg cgccgatacg cagcttattc aggatctctt
     8221 tttcccgatg agtaaggagg gctgattccg tgctgttata acgatagtta cctgaatgcg
     8281 taatcaagta gctggcaagc ttttgcgtaa agtagcattc gccgcgcagg acgccttgca
     8341 acccattgac aacacgttct tgatcctcca tggaataaaa aacgccgttg atatgaggcc
     8401 agttttcaat gtcgcggtac gggtaatctt caggcgtatt tagcaacaat attttgatat
     8461 tgttgttttt cctgctcaaa gtatcctgcc aataatggat aagcttttta tctgcttcca
     8521 tcatatccag aagaataata gagcctgaag agatatcgtc cagagaacgt tgaatattat
     8581 gtaattttcc tgtaattgcc agcgattgtt taaggtgctg caagagagct gtcgcctgca
     8641 aagaaggttt agtgatcaac aataatgtat gaccatgaat actatggact tcattaaaca
     8701 tgatgaaacc ccgctttttt tattgatcgc acacctgaca gctgcctcta aaatagaagc
     8761 accagaagta ctgacagatg ttgcactgct gtgtgtagta ataaatcagc cctaaatggg
     8821 taaaatataa aactaatgga ttacatctga tttcaatcta gccattacaa atcttaaatc
     8881 aagtgttaaa catgtaacta aatgtaacta gtt
//
LOCUS       DAZKHC020000148         8871 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZKHC020000148 DAZKHC020000000
VERSION     DAZKHC020000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.148"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(320..583)
                     /gene="rpsT"
                     /locus_tag="ACHOSM_RS21590"
                     /old_locus_tag="pgaptmp_004318"
     CDS             complement(320..583)
                     /gene="rpsT"
                     /locus_tag="ACHOSM_RS21590"
                     /old_locus_tag="pgaptmp_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGU4560684.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            686..904
                     /locus_tag="ACHOSM_RS21595"
                     /old_locus_tag="pgaptmp_004319"
     CDS             686..904
                     /locus_tag="ACHOSM_RS21595"
                     /old_locus_tag="pgaptmp_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HGU4560685.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            912..1853
                     /gene="ribF"
                     /locus_tag="ACHOSM_RS21600"
                     /old_locus_tag="pgaptmp_004320"
     CDS             912..1853
                     /gene="ribF"
                     /locus_tag="ACHOSM_RS21600"
                     /old_locus_tag="pgaptmp_004320"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGU4560686.1"
                     /translation="MKLIRCIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1896..4712
                     /gene="ileS"
                     /locus_tag="ACHOSM_RS21605"
                     /old_locus_tag="pgaptmp_004321"
     CDS             1896..4712
                     /gene="ileS"
                     /locus_tag="ACHOSM_RS21605"
                     /old_locus_tag="pgaptmp_004321"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGU4560687.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYKGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4712..5206
                     /gene="lspA"
                     /locus_tag="ACHOSM_RS21610"
                     /old_locus_tag="pgaptmp_004322"
     CDS             4712..5206
                     /gene="lspA"
                     /locus_tag="ACHOSM_RS21610"
                     /old_locus_tag="pgaptmp_004322"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGU4560688.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5331..5780
                     /gene="fkpB"
                     /locus_tag="ACHOSM_RS21615"
                     /old_locus_tag="pgaptmp_004323"
     CDS             5331..5780
                     /gene="fkpB"
                     /locus_tag="ACHOSM_RS21615"
                     /old_locus_tag="pgaptmp_004323"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4560689.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5782..6732
                     /gene="ispH"
                     /locus_tag="ACHOSM_RS21620"
                     /old_locus_tag="pgaptmp_004324"
                     /gene_synonym="lytB"
     CDS             5782..6732
                     /gene="ispH"
                     /locus_tag="ACHOSM_RS21620"
                     /old_locus_tag="pgaptmp_004324"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HGU4560690.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6798..7712
                     /gene="rihC"
                     /locus_tag="ACHOSM_RS21625"
                     /old_locus_tag="pgaptmp_004325"
     CDS             6798..7712
                     /gene="rihC"
                     /locus_tag="ACHOSM_RS21625"
                     /old_locus_tag="pgaptmp_004325"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HGU4560691.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7879..8700
                     /gene="dapB"
                     /locus_tag="ACHOSM_RS21630"
                     /old_locus_tag="pgaptmp_004326"
     CDS             7879..8700
                     /gene="dapB"
                     /locus_tag="ACHOSM_RS21630"
                     /old_locus_tag="pgaptmp_004326"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGU4560692.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGGAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         2034 a         2183 c         2501 g         2153 t
ORIGIN      
        1 aatggttaaa gtgtatagga atttaattca aaaatagagt ggtcttaagt ttaatacctc
       61 aatgtgtatc acaatatcca cattctttgt aggggtgtct ggagattgag tagatattct
      121 tgttcagaat gtatcagcag ataattctac gatttttaag ccacgaagag ttcagagagt
      181 gtaacggcat gtctgcaaga accagcatct tttgactatc tggcaaccgg cagtgtgttt
      241 tctcacgcat cacaaaagca gcaggcataa aaaaacccgc ttgcgcgggc tttttcacaa
      301 agcttcagca aattggcgat taagccagtt tgttgatctg tgcagtcagg ttagccttat
      361 gacgtgcagc tttgtttttg tggatcagac ctttagcagc ctgacggtcc acgatcggtt
      421 gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata gctgcgtata
      481 ctttcttgat gaaagtacgc atcatagagc gacggcttgc gttgtgctta cgagcctttt
      541 cagactgaat ggcgcgcttc ttagctgatt tgatattagc caaggtccaa ctcccaaatg
      601 tgttctatat ggacaattca aaggccgagg aatatgccct tttagccttc ttttgtcaat
      661 ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg atggcgcagg
      721 attctaccag cttgcggggt gtgaatacag cttttccgcg ataaaaattg cagcaggcgg
      781 tcactttctt cccgtgattt gcgccatggc aatgaaaagc cacttctttc tgatttcggt
      841 actcaatcgc cggttaacct tgaccgctgt acaaggtata ctcggacgat tttcactgtt
      901 ttgagccaga catgaagctg atacgctgca tacataatct cagccaggcc ccgcaagaag
      961 ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg cggtcatcgc gcgctgttac
     1021 agggcttgca ggaagaaggg cgcaagcgca acttaccggt gatggtgatg ctttttgaac
     1081 ctcaaccact ggaactgttt gctaccgata aagccccggc aagactgacc cggctgcggg
     1141 aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg cgtttcgaca
     1201 ggcgtttcgc ggcgttaacc gcgcaaaatt tcatcagcga tcttctggtg aagcatttgc
     1261 gcgtaaaatt tcttgccgta ggggatgatt tccgctttgg cgctggtcgt gaaggcgatt
     1321 tcttgttatt acagaaagct ggcatggaat acggcttcga tatcactagt acgcaaactt
     1381 tttgcgaagg tggcgtgcgt attagcagca ccgctgtgcg tcaggcgctg gcggatgaca
     1441 atctggctct ggcagaaagt ttactggggc acccgtttgc catctccggg cgtgtagtcc
     1501 acggtgatga attagggcgc actataggtt tcccgacggc gaatgtaccg ctacgccgtc
     1561 aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt gaaaagccgt
     1621 tacccggcgt ggcaaacatc ggaacacgcc caacggttgc cggtattcgc cagcagctgg
     1681 aagtgcattt gttagatgtt gcaatggacc tttacggtcg ccatatacaa gtagtgctgc
     1741 gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa gcgcagattg
     1801 cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac aaaaccggct taagcctgtt
     1861 atgtaatcaa accgaaacac ggaaccgaga atctgatgag tgactataaa tcaaccctga
     1921 atttgccgga aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa ccgggaatgc
     1981 tggcgcgttg gactgatgat gatctgtacg gcatcatccg tgcggctaaa aaaggcaaaa
     2041 aaaccttcat tctgcatgat ggccctcctt atgcgaatgg cagcattcat attggtcact
     2101 cggttaacaa gattctgaaa gacattatcg tgaagtccaa agggctttcc ggttatgact
     2161 cgccgtatgt gcctggctgg gactgccacg gtctgccgat cgagctgaaa gtagagcaag
     2221 aatacggtaa gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag tgccgcgaat
     2281 acgcggcgac ccaggttgac ggtcaacgca aagactttat ccgtctgggc gtgctgggcg
     2341 actggtcgca cccgtacctg accatggact tcaaaactga agccaacatc atccgcgcgc
     2401 tgggcaaaat catcggcaac ggtcacctgc acaaaggcgc gaagccagtt cactggtgcg
     2461 ttgactgccg ttctgcgctg gcggaagcgg aagttgagta ttacgacaaa acttctccgt
     2521 ccatcgacgt tgcttttcag gcggtcgatc aggatgcact gaaagcaaaa tttgccgtaa
     2581 gcaacgttaa cggcccaatc tcgctggtga tctggaccac tacgccgtgg actctgcctg
     2641 cgaaccgcgc aatctctatt gcacctgatt tcgactatgc gctggtgcag atcgacggtc
     2701 aggccgtgat tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc ggcgtgaccg
     2761 attacaccat tctcggcacg gtaaaaggtg cggagcttga gttgctgcgc tttacccatc
     2821 cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca cgttaccctg gatgccggta
     2881 ccggtgccgt tcacaccgcg cctggccacg gcccggacga ctatgtgatc ggtcagaaat
     2941 acggcctgga aaccgctaac ccggttggcc cggacggcac ttatctgccg ggcacttatc
     3001 cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg ctgctgcagg
     3061 aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc tgctggcgtc
     3121 acaaaacgcc gatcatcttc cgcgcgacgc cgcagtggtt cgtcagcatg gatcagaaag
     3181 gtctgcgtgc gcagtcactg aaagagatca aaggcgtgca gtggatcccg gactggggcc
     3241 aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc cgtcagcgca
     3301 cctggggcgt accgatgtca ctgttcgtgc acaaagacac ggaagagctg catccgcgta
     3361 ctctcgaact aatggaagaa gtggcaaaac gcgttgaagt tgacggcatc caggcgtggt
     3421 gggatcttga tgcgaaagag atcctcggcg atgaagctga tcagtacgtg aaagtgccgg
     3481 acacattgga tgtatggttt gactccggat ctacccactc ttctgttgtt gacgtacgtc
     3541 cggaatttgc cggtcacgca gcggacatgt atctggaagg ttctgaccag caccgtggtt
     3601 ggttcatgtc ttccctaatg atctccaccg cgatgaaagg caaagcgccg tatcgtcagg
     3661 tactgaccca cggctttacc gtggatggtc agggtcgcaa gatgtctaaa tccatcggca
     3721 ataccgtttc gccgcaggat gtgatgaaca aactgggcgc ggatattctg cgtctgtggg
     3781 tggcatcaac cgactacacc ggtgaaatgg ccgtttctga cgagatcctg aaacgtgctg
     3841 ccgatagcta tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac ctgaacggtt
     3901 ttgatccagc aaaagatatg gtgaaaccgg aagagatggt ggtactggat cgctgggccg
     3961 taggttgtgc gaaagcggca caggaagaca tcctcaaggc gtacgaagca tacgatttcc
     4021 acgaagtggt acagcgtctg atgcgcttct gctccgttga gatgggttcc ttctacctcg
     4081 acatcatcaa agaccgtcag tacaccgcca aagcggacag tgtggcgcgt cgtagctgcc
     4141 agactgcgct gtatcacatc gcagaagcgc tggtgcgctg gatggcacca atcctctcct
     4201 tcaccgctga tgaagtgtgg ggctacctgc cgggcgaacg tgaaaaatac gtcttcaccg
     4261 gtgagtggta caaaggcctg tttggcctgg cagacagtga agcgatgaac gatgcgttct
     4321 gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa gcgcgtgccg
     4381 acaagaaagt gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa ccggaactgt
     4441 cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc tccggcgcta
     4501 ccgttgcaga ctataacgac gcacctgctg atgctcagca gagcgaagta ctcaaagggc
     4561 tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg cactacaccc
     4621 aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc agcaacgtcg
     4681 ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt caacagggct
     4741 acgctggctg tggctggtgg tagtcgtgct gattatcgat ctgggcagca aatacctgat
     4801 cctccagaac tttgctctgg gggatacggt cccgctgttc ccgtcgctta atctgcatta
     4861 tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat agcggcggct ggcagcgttg
     4921 gttctttgcc ggtattgcga ttggtattag cgtgatcctg gcagtgatga tgtatcgctc
     4981 gaaggccacg cagaagctaa acaatatcgc ttacgcgctg attattggcg gcgcgctggg
     5041 caacctgttc gaccgcctgt ggcacggctt cgttgtcgat atgatcgact tctacgtcgg
     5101 cgactggcac ttcgccacct tcaaccttgc cgatactgcc atttgtgtcg gtgcggcact
     5161 gattgtgctg gaaggttttt taccttctaa agcgaaaaaa caataataaa ccctgccgga
     5221 tgcgatgctg acgcatctta tccggcctac agattgctgc gaaatcgtag gccggataag
     5281 gcgtttacgc cgcatccggc aaaaatcctt aaatataaga gcaaacctgc atgtctgaat
     5341 ctgtacagag caatagcgcc gtcttggtgc acttcacgct aaaactcgac gatggcacca
     5401 ccgccgagtc tacccgcaac aacggtaaac cggcgctgtt ccgcctgggt gatgcttctc
     5461 tttctgaagg gctggagcaa cacctgttgg ggctgaaagt gggcgataaa accaccttct
     5521 cgttggagcc agatgcggcg tttggcgtgc cgtcaccgga cctgattcag tacttctccc
     5581 gccgtgaatt tatggatgca ggcgagccag aaattggcgc aatcatgctt tttaccgcaa
     5641 tggatggcag tgagatgcct ggcgtgatcc gcgaaattaa cggcgactcc attaccgttg
     5701 atttcaacca tccgctggcc gggcagaccg ttcattttga tattgaagtg ctggaaatcg
     5761 atccggcact ggaggcgtaa catgcagatc ctgttggcca acccgcgtgg tttttgtgcc
     5821 ggggtagacc gcgctatcag cattgttgaa aacgcgctgg ccatttacgg cgcaccgata
     5881 tatgtccgtc acgaagtggt acataaccgc tatgtggtcg atagcttgcg tgagcgtggg
     5941 gctatcttta ttgagcagat tagcgaagta ccggacggcg cgatcctgat tttctccgca
     6001 cacggtgttt ctcaggcggt acgtaacgaa gcaaaaagtc gcgatttgac ggtatttgac
     6061 gcgacctgtc cgctggtgac caaagtgcat atggaagtcg cccgcgccag tcgccgtggc
     6121 gaagaatcta ttctcatcgg tcacgccggg cacccggaag tggaagggac aatgggccag
     6181 tacagtaacc cggaaggggg aatgtatctg gtcgaatcgc cggacgatgt gtggaaactg
     6241 acggtcaaaa acgaagagaa gctctccttt atgacccaga ccacgctgtc ggtggatgac
     6301 acgtctgatg tgatcgacgc gctgcgtaaa cgcttcccga aaattgtcgg tccgcgcaaa
     6361 gatgacatct gctacgccac gactaaccgt caggaagcgg tacgcgccct ggcagaacag
     6421 gcggaagttg tgttggtggt cggttcgaaa aactcctcca actccaaccg tctggcggag
     6481 ctggcccagc gtatgggcaa acgcgcgttt ttgattgacg atgcgaaaga catccaggaa
     6541 gagtgggtga aagaggttaa atgcgtcggc gtgactgcgg gcgcatcggc tccggatatt
     6601 ctggtgcaga atgtggtggc acgtttgcag cagctgggcg gtggtgaagc cattccgctg
     6661 gaaggccgtg aagaaaacat tgttttcgaa gtgccgaaag agctgcgtgt cgatattcgt
     6721 gaagtcgatt aagtcattag cagcctaagt tatgcgaaaa tgccggtctt gttaccggca
     6781 ttttttatgg agaaaacatg cgtttaccta tcttcctcga tactgacccc ggcattgacg
     6841 atgccgtcgc cattgccgcc gcgatttttg cacccgaact cgacctgcaa ctgatgacca
     6901 ccgtcgcggg taatgtctcg gttgagaaaa ccacccgcaa cgccctgcaa ctgctgcatt
     6961 tctggaatgc ggagattccg ctcgcccagg gagccgctgt gccgctggta cgcgcaccgc
     7021 gtgatgcggc atctgtgcac ggcgaatcgg gaatggctgg ctacgacttt gttgagcaca
     7081 accgaaagcc gctcgggata ccagcgtttc tggcgattcg ggatgccctg atgcgtgcac
     7141 cagagcccgt taccctggtg gccatcggcc cgttaaccaa tattgcgctg ttactttcac
     7201 aatgcccgga atgtaaaccg tatattcgtc gtctggtgat catgggcggt tctgctggtc
     7261 gcggcaactg cacaccaaac gccgagttta atatcgctgc cgatccagaa gcggctgcct
     7321 gtgtcttccg tagcggcatc gaaatcgtca tgtgcggtct ggatgtcacc aatcaggcaa
     7381 tattaacccc tgactatctc gctactttgc cggagttgaa tcgcaccgga aaaatgctcc
     7441 acgccctgtt tagccactac cgtagcggca gtatgcaaag tggactgcgt atgcacgatc
     7501 tttgcgctat tgcctggctg gtgcgcccgg aattgttcac tctcaaaccg tgttttgtgg
     7561 cagtggaaac tcagggcgaa tttacctcag gcacgacggt ggttgatatc gacggttgcc
     7621 tgggcaaacc agccaatgta aaggtggcat tggatctgga tgtgaaaggc ttccagcagt
     7681 gggtggctga ggtgctggct ctggcgtcgt aacctgtcac atgttatcgg catgcagtca
     7741 ttcatcgact catgcctttc actgatatcc ctccctgttt atcattaatt tctaattatc
     7801 agcgtttttg gctggcggcg tagcgatgcg ctggttactc tgaaaacggt ctatgcaaat
     7861 taacaaaaga gaatagctat gcatgatgca aacatccgcg ttgccatcgc gggagccggg
     7921 gggcgtatgg gccgccagtt gattcaggcg gcgctggcat tagaaggcgt gcagctgggc
     7981 gcggcgctgg agcgcgaagg atcttcttta ctgggcagcg acgccggtga gctggctggt
     8041 gcagggaaaa caggcgttac cgtgcaaagc agcctcgatg cggtaaaaga tgattttgat
     8101 gtgtttatcg attttacccg tccggaaggt acgctgaacc atctcgcttt ttgtcgccag
     8161 catggcaaag ggatggtgat cggcactacg gggtttgacg aagccggtaa acaggcgatt
     8221 cgtgacgccg ccgccgatat tgcgattgtc tttgccgcca attttagcgt tggcgttaac
     8281 gtcatgctta agctgctgga gaaagcagcc aaagtgatgg gtgactacac cgatatcgaa
     8341 attattgaag cacatcatag acataaagtt gatgcgccgt caggcaccgc actggcaatg
     8401 ggaggggcga tcgcccacgc ccttgataaa gatctgaaag attgcgcggt ctacagtcgt
     8461 gaaggccaca ccggtgaacg tgtgcctggc accattggtt ttgccaccgt gcgtgcaggt
     8521 gacatcgttg gcgaacatac cgcgatgttt gccgatattg gcgagcgtct ggagatcacc
     8581 cataaggcgt ccagccgcat gacatttgct aacggcgcgg taagatcggc tttgtggttg
     8641 agtggtaagg aaagtggtct ttttgatatg cgagatgtgc ttgatctcaa tagtttgtaa
     8701 ccacaaaata tttgttatgg tgcaaaaata acacatttaa tttattgatt ataaagggct
     8761 ttaatttttg gcccttttat ttttagtgtt atgtttttaa attgtctata agtgccaaaa
     8821 actacatgtt ttgtcttcgg tttttgttgt tttaatgtaa attttgacca t
//
LOCUS       DAZKHC020000149         8711 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZKHC020000149 DAZKHC020000000
VERSION     DAZKHC020000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.149"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(368..1240)
                     /locus_tag="ACHOSM_RS21635"
                     /old_locus_tag="pgaptmp_004327"
     CDS             complement(368..1240)
                     /locus_tag="ACHOSM_RS21635"
                     /old_locus_tag="pgaptmp_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU4560693.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            2473..3957
                     /locus_tag="ACHOSM_RS21640"
                     /old_locus_tag="pgaptmp_004328"
     CDS             2473..3957
                     /locus_tag="ACHOSM_RS21640"
                     /old_locus_tag="pgaptmp_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HGU4560694.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(4279..4881)
                     /locus_tag="ACHOSM_RS21645"
                     /old_locus_tag="pgaptmp_004329"
     CDS             complement(4279..4881)
                     /locus_tag="ACHOSM_RS21645"
                     /old_locus_tag="pgaptmp_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560695.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLSYRTTVDDKRT"
     gene            complement(4976..5182)
                     /locus_tag="ACHOSM_RS21650"
                     /old_locus_tag="pgaptmp_004330"
     CDS             complement(4976..5182)
                     /locus_tag="ACHOSM_RS21650"
                     /old_locus_tag="pgaptmp_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4560696.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     GRSSRWLKSEVESWLQARIAQSRP"
     gene            5924..6061
                     /locus_tag="ACHOSM_RS21655"
                     /old_locus_tag="pgaptmp_004331"
     CDS             5924..6061
                     /locus_tag="ACHOSM_RS21655"
                     /old_locus_tag="pgaptmp_004331"
                     /inference="COORDINATES: protein motif:HMM:NF017162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulating family protein"
                     /protein_id="HGU4560697.1"
                     /translation="MIFHTRYRLTPVDLEARNSAVAHRLTELTANFTIVFLLPSGHLN
                     F"
     gene            complement(6400..6600)
                     /locus_tag="ACHOSM_RS21660"
                     /old_locus_tag="pgaptmp_004332"
     CDS             complement(6400..6600)
                     /locus_tag="ACHOSM_RS21660"
                     /old_locus_tag="pgaptmp_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560698.1"
                     /translation="MVIRKKKCRDCGNAITNNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVCILCSW"
     gene            6799..7368
                     /locus_tag="ACHOSM_RS21665"
                     /old_locus_tag="pgaptmp_004333"
     CDS             6799..7368
                     /locus_tag="ACHOSM_RS21665"
                     /old_locus_tag="pgaptmp_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HGU4560699.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGEFKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            7628..8029
                     /locus_tag="ACHOSM_RS21670"
                     /old_locus_tag="pgaptmp_004334"
     CDS             7628..8029
                     /locus_tag="ACHOSM_RS21670"
                     /old_locus_tag="pgaptmp_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4560700.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQDAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            8017..8451
                     /locus_tag="ACHOSM_RS21675"
                     /old_locus_tag="pgaptmp_004335"
     CDS             8017..8451
                     /locus_tag="ACHOSM_RS21675"
                     /old_locus_tag="pgaptmp_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HGU4560701.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
BASE COUNT         2189 a         2004 c         2295 g         2223 t
ORIGIN      
        1 ttttccttac agagggtgaa taaaaaagcc ggtacccaca gatgtgggta ccggcaggta
       61 caaaacagca tggtcagata aagtggtttt tcccggacat aaatgtttcc cctctctcat
      121 ccgccagaga agcatcagtc cgttgtgtgc aattccgttc tgcatgatga agagattcaa
      181 ctgccttttc gcagaccatt aaccactata aacgatcgat agtaacgatc gatacctgtt
      241 ttatcgatcg ttttattcta ttaacggcag ggaagtcacg caagagaaag aggtgggtat
      301 gagggggggg ggtcatgttt tcagttggtt aacgcaatgt aatcaggcat ttcccctggc
      361 agagtgttca gaagaaaatc cagttccata cagcgcgggc aacagagacc accgagtcac
      421 ggacggcacg cagaaccgtg cgtgcaacag aggcaataca gacgctctcc gccgtgtcaa
      481 atatccggtc caccgcaccg gtaaactgtt cacgggcctg agaccggaca gattccgtgc
      541 gcagctcgtc ctgcaggcgg gtcatcaggg gactggcggc atggtcggga agcgctgtca
      601 tgagcgcact gaccagcgta tccagttccc agccggtgcg ggccgatacg gccacaaccg
      661 gatgtacggg ccggaacaga cggaataccg cctccgtttt ttcgcgaata ttctgtgcct
      721 gtgcgggaga aggctgaata ccggccatat cccattcatg gcagggctcc gttttgtcgg
      781 cctgcgtcac cacaaacagc acctgctgat gtccccggtg caggatgtgt cgccagaaat
      841 actcatccac agacagggcg cggtcatcgg ctttaatcag ccacagcacc aggtccagtt
      901 cgggcagaat gtcacggtac agggcttcat actctgcatc cctgtcccgg ctctcgccca
      961 ccccgggcag gtcagtgata accatgctgt gaccgtggcc actcagacgg aagcgccgca
     1021 cttcccgggt gccggcgtga acatcactga ccggggtgac ctccccctga aacagtgcat
     1081 tacagagtga ggatttaccg gccccgcttt tacccataat gccaatcacg ggttcgtgac
     1141 tggtgagttt gcgcagatgt tccaggatgt gacgggaaag tgagtaaggc agggaggaga
     1201 gcggtttttc aattgcctca atggcatcag acggattcat acatgtattc ccggtgaaaa
     1261 aagacaaaac cccgcgcacc ggaaaggtgg cgggggattc acggagataa tatctgtctg
     1321 aaattgtttt gaatcgatgg gattgttgcg tattaacatg aaggagatca ggacttatcc
     1381 gttactgggt gatctccttc catgaaaata cgccgttatt gttaaacagc aggctgattt
     1441 ctccgtaaag gggaaaatat ttatcggctg tatgattatc aggtgtgtct gtgtttactg
     1501 ctgtgagtat tttttagtgt attgtggggg agatgtcagt gggagatatt aaatttacgt
     1561 ttataattat cttacttctt ctgtgtgttt tttataagag tgcacacgta atgatatctg
     1621 ttgataccat gtggttaagt gtttcccttc ctggtcatgc gtaatgatta aatgcttttg
     1681 tgacaccatt cttccggtct gaatatttat cccgttcttt tttatttgtg cagtgatact
     1741 gtcggaagag aattcagtaa tgtgaggtgt cctggtcagg ataatatctg ctgagatatt
     1801 attagttgag tgtgttttta ctgagcaaaa gctggctttt aattcctgta ctgaaacact
     1861 gtcgctcgtc cggggttaac tgaaactgga tgatgatgta aaatgaaggc tgtttttgtg
     1921 tttgtcgaag aagtgttatt ttaatccatt ctcatcattg gctggcgatt atacagaagt
     1981 atttgaagaa aaaatggtgc attttatctg tgtcagcagg gaagaagtat tttattgatg
     2041 tatcagtatt aaatggtttt tgttatttat aaaaatgtca tcctgtccat agaatattgt
     2101 actcagcatt cagtgcagga actgtagtct tctttatttt attcttcttc aggagggggg
     2161 ggtgattcct gaaggcgtta ttcccggtgt tctgtttatg attcttcccg gcaggaaacc
     2221 ggtatctgca atacccggga tgatgctttt tgttcgtacg tggttgtgta aaatttgaat
     2281 agtggatgtg ctccgaaggg aaggagggga cacacagatt cattttgtct ttcttcagca
     2341 gcaaaataca ctgtggttat tatttaacca ttaaatcaac aaaaattttt acctcatatt
     2401 gatttgactg ctatttttgc tattaatagc cagaattgca gtgcttttat atctgtgtat
     2461 agtgaggtta ctatgtcatt cggtcagcct tgtgatgagt ttcccctgag ttcgctgcct
     2521 ccactcattc gtgatgccgt tattgaagct cagcaaatta cgcaggctcc ccttgggctt
     2581 gtggcagcgt ctgccctggg cgctgtttca cttgtctgcc agaatctgat tgatgtctgc
     2641 agactgaata cactgcgggg gccggtctct ctttttttcc tgacgcttgc cgagtccgga
     2701 gaacgaaaaa ccgcagtgga caaattatta atgaagcccc tgtaccagca ggagatgcag
     2761 ttatattcca gatacaaaag tgaactggct gtctggaaaa acaaggaaga gcttttaaag
     2821 gctcagaaaa aagcattgtt gtcaaaactg aataaagaac tccgcaaagg cgcggatgag
     2881 tcagaaaccc tcagacaact ggaagtactt cagaaaaaca gtgcggaaga acctgtgagg
     2941 tataaattca tttttaatga tgccacgact gcggcaatta agaatcagct ttgtggtaaa
     3001 tggcgttcag ttggtattat gtcagatgaa gccggtatta tatttgatgg atataccctg
     3061 tctgagttgc cttttattaa caaaatgtgg gatggctctg tattgtctgt ggacagaaag
     3121 aatgaacccg aacagatgat tgaaaatgcc agaatgacgt tatccctcat ggtgcaaccc
     3181 ggtctttttg accgatatat ggagcgaaaa ggttctgttg ccagggacag tggttttctt
     3241 gcccgttgtc ttatcagtaa acctgcaacc acgcagggga aacgctttat taacggagct
     3301 gtgattccgg gaggatcatt aacggcattt catgaacgat taatggaact ggccagaggc
     3361 agtattgaaa aatccagtga ggatgaacgg tattgtctgc atttttcgcc tgaagcgcaa
     3421 aaaatattta ttgagcacta taatgttctg gagcaggatt tatccccttc cggaccctta
     3481 agcccgtttc ggggacatgt ttcaaaaaaa acagaaaaca ttgcaagaat tgcagcgtta
     3541 tttcagtatt tcagctatgg tgaagggaaa atctctgccg atatcatgac gtctgctgtc
     3601 gtaataagtt cgtggtacac agatgaatat aaaaaattat ttgcgttacc tgatgaatct
     3661 gaactgcagc agaaggacgc cgaggagctg ttcgactggc tcattgagga gtgccgcgga
     3721 gaatgcccac cccgtgtcag gaaaaattat attctccagt gtggcccggg ccggttcaga
     3781 aacaggaaaa aactgaacgc attgctgaac attcttgaat ctcagttcag gctttctgtc
     3841 gtgccggaag gaaaaaccat gtatgttctg cttccccaga tagccagttt aaagttgtct
     3901 gatgtctctg gtatttttac ctcgggttat cactacaata agctgagagc gaagtaaccg
     3961 gtgagcagtt tttctccatt atgcttcaga cttagatgat tgattattat ggggatttca
     4021 tcaccgatat tcagatgtat gaatatcggt gatgaactaa agctcaggag ctgcgtggtg
     4081 gtgatgaatc aacaggcata taactgatac ccgccggtca gaagttccag cctgcgactt
     4141 tcctgtctgc gtgcccgcag tccggcgttc aggtgcagcg tgtgacagaa cgcacggaga
     4201 gcgaaacggc aggcagacag gccgttcagt cagccctggc accctgcccg gataccagct
     4261 tccctgaaac aactggcatc aggtgcgctt atcgtcaacc gttgtgcggt atgacaggag
     4321 cacgtactga tccatcagat ccgtcagtgc tgcgctctgc ttctgacggt tgatgatcca
     4381 ttcctccagc tctttttctg atttccgttt cagattctcc gtccagtcgt caaggcagtt
     4441 tccggtcatt aaatcaagcc agcaaagcca gaccagtttg aggcgcaact ccgtgccacc
     4501 aaactgacgg aagaggctga tcagtttatt tgccagagaa cgctgcctca gacgttcaca
     4561 ttcagacagg agaatgcctt ccagcatcgg atgatgaacc agcagatgtt cactggtgca
     4621 taaattcact atcagatcat catccagggg cggtgtggcc cgtttgtcag ggccataaat
     4681 cagccggtgc aggctctggg gtacgatggc aatctccggt ttttcaccat tgaatgacac
     4741 ccaaccgggc gtcagattat ggaagtagta gatattcgga tgacacacgc cccaggatac
     4801 ggcgtgccgg gtgaagccgg tcagccattt ctggtagctt tcgtggagcc attcgtagcg
     4861 ggtctgatac tgttgtttca ttttcatatt cctcacgggg atataacagg cagcagaatg
     4921 ccgccggcag gccggagatt acgtctgctg cgggtgaacg gataacggtc agaaattacg
     4981 gacgggactg tgcaatacgc gcctgcagcc aggattccac ttcacttttc agccagcggg
     5041 agctgcgccc cattttgatg ggggcaggaa agcccccatc tttgatgagt ttgtaaaacc
     5101 acttatcggt taagccggta agttgagtga taaacgccat gtcgaccatc tggtcatcca
     5161 tcagcgaaac aggggtagcc atgagaatgt tcctccggat gttgttaaac cagccggtgt
     5221 ggtcatgaaa acagcgcgcc accttcaccg gcataacaac cggggagaaa cgtaaaaaat
     5281 aatcgggaat gtggctgtcc ggagataaac ggggggattg cgatggcaga gccgtagcgg
     5341 aatcccgggt ggttgttgcg aaaagatgct tccgcacgca cgcgcgataa aggagggagg
     5401 actttcgtga gacaagggga aaactagcgt tctgataatt gcagagttgt tttcggaggt
     5461 ggttacagtg agtcagcccg ttcctggcga ggaggagact gatatcaccg atgggataag
     5521 gctgtctgat tggtattata aatataagaa gtcatcagag agtaacgtaa aggatttgcc
     5581 agagtaagct aagagagtaa atctctccgg taacagagcg ctgtgaagca ttctgtatca
     5641 gagtgcaaat acatgaacgc tgatgcgtaa aatcagcagt gacacgccag ctccccctat
     5701 gcccattcct gtataagaca tgaactgagc gtattaatgc tcctgctctg agactgagcc
     5761 ggtgagtgag gggctgagaa atccctcttg acacagctct aaccagaaat acttccccgg
     5821 ttgttgcggt gcgtccggat ccccgataac ggaacactaa ggctgtattg cgggacaccg
     5881 ggtaagtggc agcctgtaat gtgttctgtc aacgccagaa aaaatgattt ttcacacgcg
     5941 ttatagactc actcctgtgg acctggaagc ccgtaactct gcggttgccc accgactgac
     6001 ggaactgacc gcaaacttta cgatcgtgtt cctgcttcct tctggtcacc tgaactttta
     6061 gccagcgcta cgataagtcc ggtgttatct gcaccggagg accgggaaca ttaagcggta
     6121 tagcgacaac aaagaattcg aaatcaaaag ggaatggtgc cggaggcgtg ggcggtaata
     6181 ggctgatttt caggtattac cggcttacaa cgtgtggttg atattgaggg gctgtgagtt
     6241 tcgcgtattt gtcagaaacc cagatgagtt ttcctgtatg ggacgaaata aggcagcggc
     6301 aatacctgga aaaatttaat atggtatgaa ggagaacctg tcggttacgc ttaatataca
     6361 cggtacactg tcagctcagg ggatacatga accgttatct caccagctac acagtataca
     6421 cacactgacg ctgacagtag tcagcaggac cacaataatc agcgccagca gtatggtaac
     6481 gatacggtct gtattccggt agtagccgaa ggggtcgaca gaaccacaat acgggcaaca
     6541 cactgtatta ttagtaatcg cgtttccaca gtccctgcac ttctttttgc gtatcaccat
     6601 aactccctcc tcacagcctt acccgtaacc ggttttttac attaaaaaac aacgctgaaa
     6661 attcaaccgt cagtccggag acgaccgttc gggttatcac agagtgcctg agacagtgtc
     6721 ctgccggagg tcacttcagg gactctgcgc attttttacg acgtggctat tccgtatgaa
     6781 ccatacggag atttaaccat gacttacaaa tacaacccct tctggcagca acgtattcgt
     6841 gagacggtgc ggcacgcact gaatgttcat ccccgcctga cggcattgcg ggttgacctg
     6901 cgtctcccgg atgtaccggc agcaacggac gcagctgtga tatcccgctt catcaatgcc
     6961 ctgaaagccc gaatcgacgc ttaccagaaa cgtaagcatc gggaaggtaa acgcgtgcat
     7021 cccaccaccc tgcattacgt ctgggcccgg gagtttgggg agttcaaagg taaaaaacac
     7081 tatcacctga tgctgctggt caaccgggat acctggtgtc gtgccggtga ttaccgcgct
     7141 ccggagtctc tggccgggat gattaaacag gcgtggtgca gtgccctggg agtggatgtc
     7201 gggtgccatg ccacgctggt gcattttccg gcctggccgg cggtgtggct ggcgcgtaat
     7261 gatgacaccg gctttcagca agtgctggaa cgtgctgact atctggcgaa ggagcatacc
     7321 aaagctcact gcaccggtga gcgcaatttt ggctgtagcc ggagttgagc acgactggcg
     7381 cgccaatatc cggcaaacca aaagtatggg ttgttgtctg gcggcaacga tgtatccact
     7441 cgcccactga ctgtttttta cgtcagtgtt ccattgtgtg acaaccacag tgctgtaaca
     7501 gcaaccagcc tgctttaccc aaactccagt ccagatttta actgaaccgc cattcacacc
     7561 ctgaccacgc tgcccgtacc gggacggctg ttgcctgcgt gtcgtctggc ggctaaggaa
     7621 tatgactatg tacgcaaaat cctttatcgc tcttgatggc aacggacgtc tgacgggcgc
     7681 ccgtactgca caggacgcac cttatgctaa ctacacctgc cacttgtgtg gcagtgcact
     7741 cagataccat ccgcaatacg acactgaact tccctggttt gaacacactg acgacaggct
     7801 gacagagcac ggtcaacagt gcccttatgt caggccggag cgcagagaaa tacagttgat
     7861 taaacgtctg cagcaattcg taccggatgc cttacccgtg gtgcgtaaag ccagctggca
     7921 ctgcagacaa tgtcaccacg attattatgg ggagcagtac tgcacacact gccagaccgg
     7981 aggattcagt attccccgga caactcagga ggaaatatgc gaattctgaa ctgttatatg
     8041 gcgaatgaca gcaaaggcca ttttgttacg gcgaaagaag ctgcgaagca caaccgacag
     8101 gacgttttgt gctgtgtgtc ctgtggatgc ccgttaacac ttcagcgggg caatgacgga
     8161 caaccaccgt ggtttgaaca tgaccagatg actgtcgctg aaaaaatcct gctgcgatgt
     8221 acctggcttg acccggcgga gaaagaggcc cgtcgtttgc atctgcaggg catgacggtt
     8281 ccggattata cggtgaaggt gagaaagtgg ttttgtgtga tgtgtgacga agattatgag
     8341 ggggaaaagt gctgcccacg ctgcggtacc ggggtataca gcagggcgtg ggggcggcag
     8401 gaggtgccgt cggaagatgc cagggctgat aatccgttac agaggctgta atggttgcct
     8461 ccggagcagt ttgcggtgat gcctttcccg ggaatggatt gccagtgcgg ggactgtgga
     8521 gcttaattcc ggtgttggtg gcctttccgt tgattttatg ccaacagccc cctgtgactg
     8581 acaggctgcc tcgtcattcc attcgtatct ccagtaacag ggggttgtat tgtattttat
     8641 tgtgccggtt caggtgtgcg tttcccggcg cgtctgcacc ggcttaacca aattcaacag
     8701 ggataaaata g
//
LOCUS       DAZKHC020000150         8650 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZKHC020000150 DAZKHC020000000
VERSION     DAZKHC020000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.150"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            471..680
                     /locus_tag="ACHOSM_RS21680"
                     /old_locus_tag="pgaptmp_004336"
     CDS             471..680
                     /locus_tag="ACHOSM_RS21680"
                     /old_locus_tag="pgaptmp_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560702.1"
                     /translation="MKQEIILKYGNEKLTLTEDVLNATAYALGITPTELKKQCERLYK
                     AESNKPSHQDLINAKAQQIKQSKEK"
     gene            680..1054
                     /locus_tag="ACHOSM_RS21685"
                     /old_locus_tag="pgaptmp_004337"
     CDS             680..1054
                     /locus_tag="ACHOSM_RS21685"
                     /old_locus_tag="pgaptmp_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560703.1"
                     /translation="MAILKLSDVFLHRKSITDVVSYATDPYAAVESKKFAALAQDVTC
                     GDLIDSSGAKYVSGADVLVVVSDFVTADSNKTVNVLRANAGLVCIKADALNAEGAVKE
                     AAIAALAAKGFQLEGFHSVFTS"
     gene            1071..2099
                     /locus_tag="ACHOSM_RS21690"
                     /old_locus_tag="pgaptmp_004338"
     CDS             1071..2099
                     /locus_tag="ACHOSM_RS21690"
                     /old_locus_tag="pgaptmp_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4560704.1"
                     /translation="MIIGNEVVDLAPLFELNDSRNYLLSTLDFTDPVPVESHKVAVSQ
                     LIESNESLFNHPTSRFSSEHNVTKRENGEEYLIEIPYFLREDLIKPQDVQGKRKAGSS
                     IEETLPDLYAEYIAKHNVAFQRTKESILAASLFSGKTHTPKTDDVLIDYSTLFGVSPM
                     TATVDASSTNTMTVFKQFDQMHTDMIAKAQSQAATIERVVVFCKPAAFNAIRYSVGMV
                     NAFQYASPVDDANVVFNVRSLLPGVTTFTIPGTNIDLIKVTDPLLLAHMTADAVAIPK
                     LAKGAGVYQAIFGAPSSSFDLLSQGAKEYYSYAFESNRGDAVHVVTENSSLVVNHGVG
                     FSVQITVA"
     gene            2252..2449
                     /locus_tag="ACHOSM_RS21695"
                     /old_locus_tag="pgaptmp_004339"
     CDS             2252..2449
                     /locus_tag="ACHOSM_RS21695"
                     /old_locus_tag="pgaptmp_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560705.1"
                     /translation="MNTENSQALILKSVKELAAISDDSIINVSALCRMLSIDANNVRQ
                     RVFQTGCSTFEAIQYYCSKKQ"
     gene            2484..4325
                     /locus_tag="ACHOSM_RS21700"
                     /old_locus_tag="pgaptmp_004340"
     CDS             2484..4325
                     /locus_tag="ACHOSM_RS21700"
                     /old_locus_tag="pgaptmp_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560706.1"
                     /translation="MLELNVARLSNVVTFEVNRPSLEKAKAEVEKLRKQMAAVKDIEL
                     RVKTHKQSTAKAKKDVDDIAKKQAQADKANAKAQLAAQRVVAREQKALAARKEKAELK
                     LLDVGASISAMHRLSVAEQYKAIAQAREIALQYERGAISLARMNSQMKRLQQQQRKIN
                     GNRNAHAKAYAPVKGSGSIGGGAAAVMLGGLGAGATYMAMSKASEFVTNSFANAETLG
                     ELYSRAKLGGVDVNQMNNIEQWAYKNGVDSMMGEQGRRKYLDQMKDVREKATKSYDEA
                     EYVVDKKTGKGEWKGGDSGINTLMNEGFLTKKDLKDFADNPAGLVSKAVQGMVAKGYS
                     DAQIGSRLEDLADDLMLTSKYWTRSAKEVEQSAREQRESGRWVTQAQQESVIKFRELN
                     NALSGLSDSQGIAFVDGFMKSLDPKVIEEFKKSMVAMLPMFTKLGEAIGGLVNAVMKT
                     INWLMKNDEKTEAIQKNLGDAPPLSNEGMKQNLSNLTPDQYKGAGTGTIKPDNSNSLV
                     NTIKSWFMPEETVGGAQAVNQYSLEGQNIANLKQSAAQQAFKAPSYTFAPVINFNPEL
                     QVNAEVPLTIESDTGRLSEFIDFKSKASSAEFSKLLTLGVMSGGSTY"
     gene            4373..5050
                     /locus_tag="ACHOSM_RS21705"
                     /old_locus_tag="pgaptmp_004341"
     CDS             4373..5050
                     /locus_tag="ACHOSM_RS21705"
                     /old_locus_tag="pgaptmp_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HGU4560707.1"
                     /translation="MAMGPLTIGRPTEAKINSTSNDSNGRTTKGGNGFAIIASNLGSG
                     ANAAYNDYQALSFDSVESTKISRNADVTSYAVESGSEVSDHVQIRNNKFTLQGRISET
                     VLKLNPDMIKNAGINGNRRMLMLEYLNQLMDSRQPFLLVTELKNYDNVVLVGMSYEEE
                     ASESLLFTLDFEQIRLVSKATTSAIAVKTAPNKSVGGQVKMQVNTTEQKNQKSPGQDV
                     VTPVFKQ"
     gene            complement(5144..6364)
                     /locus_tag="ACHOSM_RS21710"
                     /old_locus_tag="pgaptmp_004342"
     CDS             complement(5144..6364)
                     /locus_tag="ACHOSM_RS21710"
                     /old_locus_tag="pgaptmp_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4560708.1"
                     /translation="MSIKSQAYLFKRKDGIWMFQIFVPKYLRHLYPYKLWRKSTSTRD
                     LNEARRFRNHLLLEWEELKRKHNPDNPERRLQKAIESLHSEARANKNTQRAVKRGYAS
                     PSLCLIRDEYAECYRERRSYSTLQKSARAVELFLQTIQKDDIHIEKIGRRLVTVFLDD
                     QKQRGAADQSRQNWLTCLGSLYEFAKRRYDGIPDSNPFHGHNLEARRTIESYQPFTPE
                     QMQKLLDAAEPEIRNIILMGLFSGCRLDELASLKKSEVVTVEGIRCFYISKSKTKAGI
                     RHVPIHSRLSIIVDEYLNHNYGEYLFPHSNKVKRIDGKKGPFYSQAFSRLRRSVLPIA
                     TDRQCFHSLRGMFITCLDRAGVPEQRIGAITGHTEQRAKTEAFRTYSQGASIPELAEY
                     VELVRYELNIDFKA"
     gene            6705..8314
                     /locus_tag="ACHOSM_RS21715"
                     /old_locus_tag="pgaptmp_004343"
                     /pseudo
     CDS             6705..8314
                     /locus_tag="ACHOSM_RS21715"
                     /old_locus_tag="pgaptmp_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8381..8560
                     /locus_tag="ACHOSM_RS21720"
                     /old_locus_tag="pgaptmp_004344"
     CDS             8381..8560
                     /locus_tag="ACHOSM_RS21720"
                     /old_locus_tag="pgaptmp_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HGU4560709.1"
                     /translation="MINKLLLAYLIGLVVTSTFIFIFSEEKVTYRLFATIITGLTWPL
                     SLIPSIISLMIRKSD"
BASE COUNT         2699 a         1744 c         1839 g         2368 t
ORIGIN      
        1 tgcaataacc tttggaaata ttgatagcaa aacgggtttt atagactgca aatctataat
       61 ctttggttgt aaaaacgctt tttctgaaac ggtagattat tgttggtgta gttaatccct
      121 attaaaattt aatctgtgag tagattggaa tagtgattcc ttaaaatctg actggtgcat
      181 ccacaccatg aaagatagcg gttagccacc gcacccgaat agatattcct gattgattcc
      241 gcatgttttc ctaagagcat gttttcagga atatctgttc accctttaaa cctatcttta
      301 agcatgttta ataagcattc ttagagatac gtttaaaacc tcctcaaggt tttacttatc
      361 gctgtgtatt gtgcccgaca cgttaacaaa tccccctcgc tattctcaga ttgaacctct
      421 tatagcctgg gggatttatt aaattccaac ataatggaat cgttatataa atgaaacaag
      481 aaattatcct gaaatatggc aatgaaaagt taacactaac tgaagatgtg ttgaatgcta
      541 cagcctacgc gcttggtatc actcccacag aattaaagaa acagtgtgaa cgtctttata
      601 aggctgaaag caataaacca tctcaccaag atttgattaa tgctaaagct caacaaatca
      661 aacaatctaa ggaaaaataa tggctatttt aaaattaagc gatgttttcc tacatcgtaa
      721 aagcatcact gacgtagtga gctatgcaac cgacccttac gcggctgtag aaagcaaaaa
      781 atttgcagca cttgcacaag atgtaacgtg cggcgacctc attgattcat ccggcgctaa
      841 atatgttagc ggcgctgatg ttcttgtcgt tgtgtctgac ttcgtaactg ctgattcaaa
      901 taaaacagtt aacgtgcttc gtgctaatgc cggactcgtt tgcatcaaag ctgatgccct
      961 gaatgccgaa ggcgctgtaa aagaagctgc tattgcagct ctcgctgcta aaggcttcca
     1021 gcttgaaggc ttccactctg ttttcacatc ttaagaagga ataaataata atgattatcg
     1081 gaaatgaagt ggttgatctt gctccactgt tcgaattaaa cgactcacgc aattacctac
     1141 tgtctactct ggattttact gatcctgtgc ctgtagaaag tcacaaagtg gctgtttccc
     1201 agcttatcga gagcaatgaa tctctgttca accatcctac aagccgtttt agctctgagc
     1261 ataacgttac taagcgtgag aacggggaag aatatcttat tgaaatcccg tactttttaa
     1321 gggaagacct gatcaaaccg caggacgtgc aaggcaaacg caaagccgga agctctattg
     1381 aagaaacact gccggatctg tatgcggaat atatcgctaa acacaatgtt gcattccagc
     1441 gcactaaaga aagcatcctc gcggcttctc tgttctctgg caagacacac accccgaaaa
     1501 ctgacgatgt tctgatcgat tacagcactc tgttcggtgt gtctccaatg actgcaaccg
     1561 ttgacgcatc cagcaccaac acaatgaccg tattcaagca atttgatcag atgcatacgg
     1621 atatgattgc taaggcacaa agccaggctg caaccattga gcgtgttgtt gtgttctgta
     1681 aacctgcggc attcaacgct attcgttaca gcgttggcat ggttaatgcg ttccagtatg
     1741 caagccctgt agacgatgca aacgtggtgt ttaatgtgcg ttctctgctg cctggtgtaa
     1801 ctactttcac cattccagga actaacattg acctgatcaa agtgactgat ccgctgctgc
     1861 tggcacatat gaccgctgac gctgtagcta ttcctaaact tgctaagggg gcaggtgttt
     1921 accaagctat ttttggtgcg ccttccagtt cattcgatct gctgtctcaa ggcgctaaag
     1981 agtattacag ctatgctttc gaatcgaatc gcggcgacgc agtgcacgta gtgaccgaga
     2041 atagctcgct tgtggttaac cacggtgttg gcttctctgt gcagatcaca gtagcgtaaa
     2101 aagtgttaaa gacgttcccc tacgtctgga aataatcggg gcaataaggg taagggataa
     2161 tcatataagc aaaatatgcg ctgtctcttg ccctttttgt ttttctactg tctacaaacc
     2221 taaataaaaa tatatatgtg agagaaaacc aatgaacaca gaaaattcac aggcattaat
     2281 tcttaaatct gtaaaagaac tggcagctat tagcgatgac tcaataatca atgtcagtgc
     2341 cttatgcaga atgctttcaa ttgatgcaaa taatgtgcgt cagcgtgtct ttcagactgg
     2401 atgtagcact ttcgaagcaa tccaatatta ttgctctaaa aaacaataac aaataacaat
     2461 cataatacag ggaaacaatt aacatgttag agctaaacgt ggcacgcctc tcgaacgttg
     2521 tcaccttcga agttaaccga ccatcattag agaaagcaaa agcagaagta gaaaaacttc
     2581 gtaaacaaat ggcggcggtg aaagatatag aattgcgtgt taagacacat aaacaatcaa
     2641 ccgctaaagc aaaaaaagac gtagacgata ttgctaaaaa gcaagcacag gctgataaag
     2701 ctaatgctaa agcacaatta gccgctcaac gtgtagttgc aagagaacaa aaagccctgg
     2761 cagcacgcaa agagaaggca gaattaaagc ttttagacgt tggagcaagc attagtgcaa
     2821 tgcaccgtct ttctgtagcg gaacaatata aagctattgc acaggctaga gaaattgccc
     2881 tccaatatga aagaggcgct attagtctgg cacgtatgaa tagccaaatg aaacgcctac
     2941 aacagcaaca acgtaagatt aatggcaatc gtaacgctca cgctaaagcc tatgcacctg
     3001 ttaagggtag cgggagtatt ggcggcggtg ctgcggctgt tatgctgggt ggcttaggcg
     3061 ctggcgctac gtatatggct atgagcaaag cctctgaatt tgttactaac agctttgcga
     3121 atgctgaaac gctgggtgaa ttgtatagcc gtgcgaagct gggcggtgta gacgttaacc
     3181 agatgaacaa tattgagcaa tgggcctaca aaaacggcgt tgattcaatg atgggcgaac
     3241 aaggtcgtag gaagtatctc gatcagatga aagatgtacg ggagaaggct actaaatctt
     3301 atgatgaggc tgaatatgtc gttgataaga aaactggtaa aggagaatgg aagggcggcg
     3361 atagcggcat caatacttta atgaatgaag gattcctaac taaaaaggat ctaaaagact
     3421 ttgctgataa tccggctggt ctggttagta aagcagttca gggaatggta gctaaaggct
     3481 attcagatgc acaaatcggt agtcgtctgg aagatctggc tgatgatttg atgctcacct
     3541 ctaaatactg gacacgctcc gctaaagaag tggaacaatc agcacgagaa caacgagaat
     3601 cagggcgctg ggtaacgcag gcacaacagg aaagcgttat taaattccgt gaactgaata
     3661 atgcacttag tgggctttct gactcacaag ggatcgcctt tgttgatggc tttatgaaat
     3721 ctcttgatcc gaaagtgatt gaagagttta aaaagagcat ggttgcaatg ctgccaatgt
     3781 ttaccaaatt aggcgaggct atcggcggat tggttaacgc cgtaatgaaa acaatcaact
     3841 ggctgatgaa aaacgacgag aagacggaag ctattcagaa gaatttaggg gatgcaccgc
     3901 cactatctaa cgaaggaatg aaacagaatc tttctaatct cactcctgat caatataaag
     3961 gcgctggcac cggaacaatt aagccagata acagcaattc cctggttaac actattaaaa
     4021 gctggtttat gccagaagaa acggtaggtg gggcgcaggc tgtaaatcaa tacagtctcg
     4081 aaggacaaaa cattgcaaat ctgaaacaga gtgcagcaca acaagctttc aaggctcctt
     4141 cttacacctt tgcacctgtt attaacttca atccagaatt acaagtaaat gcagaagtgc
     4201 cgctaactat cgaatcagac accggacgat tgagcgaatt tatcgacttc aaatcaaagg
     4261 catcctccgc tgagtttagc aagctattaa cgctgggtgt tatgtcaggc gggtcaacct
     4321 attaatcatc actgtggggc agtttagccc ccttaaataa ggattacaaa ttatggcaat
     4381 gggtccgtta accattggtc gcccgactga ggcgaaaatc aacagcacaa gcaacgatag
     4441 caacggtaga acaactaaag ggggtaacgg ctttgcgatc attgcctcta acctcggcag
     4501 tggtgcaaat gctgcttaca atgactacca ggcattatca tttgatagcg tggaatcgac
     4561 gaaaattagc agaaatgcag acgttacaag ctatgctgtc gaatcaggat cagaagttag
     4621 tgatcatgtt caaatacgga ataacaagtt cacattacag gggcgtatct ctgaaaccgt
     4681 attgaagctc aatcccgata tgattaagaa tgcgggaatt aacggcaata gacgtatgct
     4741 tatgcttgaa tacctaaatc agttgatgga cagtcgccaa cctttcctac ttgtcactga
     4801 gttaaaaaat tatgacaacg ttgtgttagt tggcatgtct tacgaagaag aagcaagcga
     4861 aagcctattg tttactcttg attttgagca aatccgtctt gtctctaaag caactacaag
     4921 cgcaattgcg gtaaaaacag caccaaataa atctgtaggg ggacaggtta aaatgcaggt
     4981 aaatacaaca gaacagaaaa atcaaaaaag cccagggcaa gacgttgtaa cgccagtatt
     5041 taaacaatag ccttaaacac ctatgaaacg ctctagaacg agataatcat aaaagaggca
     5101 tcaatctata ttgaagcctc tttattacgt ctataacgtt gttttacgcc ttaaaatcaa
     5161 tatttaattc atatcttact aattctacat attcagctaa ttctggaatg cttgcccctt
     5221 gtgagtaggt gcgaaaggct tctgttttag ctctctgctc tgtatgtccg gtaatggctc
     5281 cgatccgctg ttcaggcact ccggcacgat caagacaagt gataaacatc ccgcgcaagc
     5341 tgtgaaagca ttgtctatct gtagcgattg gcaagacgct acgacgtaaa cgtgagaatg
     5401 cttgagaata gaacgggcct ttcttgccgt cgattctttt tactttgttc gagtgaggga
     5461 acaaatattc accataatta tgatttaaat attcatcgac aataatcgat agtcttgaat
     5521 gtatcggaac gtgtctgatc cctgctttag tcttactctt gctgatataa aaacagcgta
     5581 ttccttccac agttacaacc tctgactttt tcaggcttgc cagttcgtct aatctgcatc
     5641 ctgaaaacaa gcccattaaa ataatatttc tgatctccgg ctccgctgca tcaagtagct
     5701 tttgcatttg ttcaggtgta aacggttgat aactctcgat tgtgcgacgt gcttcgaggt
     5761 tatgaccgtg aaaagggtta ctgtctggta ttccgtcata acggcgttta gcaaattcat
     5821 agagcgatcc taagcatgta agccagtttt gacgagattg atcggctgct cctcgttgct
     5881 tctggtcatc taagaatact gttacaagcc gtctgcctat tttctcaata tgaatatcat
     5941 ctttctgaat ggtttgtaaa aatagctcaa cggcccttgc tgatttctgt agtgttgagt
     6001 atgaacgacg ctctctgtag cattcggcgt attcgtcacg tattaagcat agggaaggcg
     6061 atgcataacc gcgttttacg gctctttgcg tgtttttatt ggcccttgcc tctgaatgta
     6121 aggattcaat agctttttgt aacctccttt cggggttatc aggattatgt ttccgcttta
     6181 gttcctccca ttctaataac agatggtttc tgaatcttct cgcctcattc agatctcgtg
     6241 tagatgtgct ttttctccac aacttgtaag ggtaaagatg gcgtaagtat ttaggaacaa
     6301 aaatctgaaa catccagatc ccatcttttc tcttaaataa gtatgcttgg ctcttgatcg
     6361 acacgataat gatcctctat tatcacgtaa tcagttacct atgattgatc acaaaatctt
     6421 tattttacag caggttatta tgggtttcct cgattccctt cgcccgctcc aatttatctt
     6481 cacctcatca atctgccatt tttgctgtat ctgattttct atattatctt aattcaatcg
     6541 cttcagactt ttcgcaatcg ccgtaatatt tccccatttt gttgtaaata aaaagcgttt
     6601 acgcaatgca atccttttgt aacaacccag gtaaaacacc ttattttcaa gttgttaaat
     6661 aagtggttat ctacgctttt aacaaacgat tgatggaata ttttatgaaa gggaaatctg
     6721 cattaactct tctgctcgct ggtatatttt cgtgcggcac atgccaggcg acaggggcag
     6781 aagtgacgag tgagtctgta tttaacattc ttaattccac ggggcagcaa cagataaaag
     6841 ttatttatcg ttaaatccag ataagtaccc taattatcgt cttctgattc actcagccaa
     6901 attaaaaaat gaaataaaga gccactatac caaagacgaa attcaagggt tgttaacatt
     6961 aacagaaaac acacgaaagt taacgttgac ggagaaaccc tggggaacat ttattcttgc
     7021 ttctactttc gaagatgaca aaacagcggc agaaacccac tttgatgctg tctggctgag
     7081 ggatagttta tggggctata tggcattagt atcagatcag ggaaatagtg tagccgcaaa
     7141 aaaggttctg ctcaccctgt gggattatat gtctacgccc gaccagatta agcggatgca
     7201 ggacgtcatt agcaacccca aacgactgga tggagttccc gggcaaatga atgctgtgca
     7261 tatccgattt gacagcaact cccccgtgat ggcagatgtg caggaagaag gcaagccgca
     7321 gctgtggaac cataaacaaa atgatgcgtt aggtctttat cttgatctcc ttatacaagc
     7381 aatcgacact ggtacaatta acgctgaaga ttggcaaaaa ggtgaccgat taaaatcggt
     7441 tgcgctgctg attgcttatc tggataaagc caatttctat gttatggaag attccggagc
     7501 atgggaagaa gatgcccgat taaacacatc ttccgttgca ctggtgacct ctggactgga
     7561 acgtttgtcg aacttactct cgaagaaaga ctctgttttt gtctctgatt tactacgcga
     7621 agctaaagcc aatgaactgg atgaacctct ttcaacaacg aggttgaacc acctcataga
     7681 taaaggttat gaaagaataa cgttacagct tgatttaggg ggagaatccc caggttatct
     7741 ggaaaaagat aaacattaca gagaagccga tgcggcttta cttaatgtta tttatcctgc
     7801 aaatcttgca aagataaaca ccagaagaaa agagcaggtt ctaaaaatag ttaaaaaact
     7861 agcaggtccg tatggtataa aaagatacga gaaggataat tatcagtcgg caaatttttg
     7921 gttcaatgat attaaaaccg ataccgatca aaattcacat tcgaaaagag aaaagagctt
     7981 cattccatca acagaagcag aatggttttt tgactcatgg tatgcaaaat cagcagcaat
     8041 tgtttataaa gaatcaagaa aagaagagta tttgaatgac tcagtgcaat ttatgaatag
     8101 atcattggcc caaataacag gcgagaatat gattggtgca aatggcagga gtgtacctga
     8161 aatggcactt cccgaaagct ataactatat ccacaagtcg ggaactttgc atgaagcgcc
     8221 aagccctatc attccgctaa attggtcgaa agcttcaatg actctcatgt tgaaagagat
     8281 gtccagcctt atcaatgatg agggaaataa atagttttca taatgaggcg gtcctggatt
     8341 aaaaggaccg ctcaaagaga aatcgaaagg ggaggtaaaa gtgatcaata aattactgtt
     8401 agcttacctg ataggtctgg ttgtgacatc gacttttata tttatcttct cggaagaaaa
     8461 agtaacatat cggttgtttg ctacaataat tacaggttta acctggccgc ttagtctcat
     8521 tccttccatt ataagtctca tgattcgtaa atccgactaa caagaaaatt atatgatcga
     8581 ttaagaaaaa tagaatcgaa caagagaata aaatatatgc ggggagtaac aaaacactcg
     8641 cagtgtgtta
//
LOCUS       DAZKHC020000151         8648 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZKHC020000151 DAZKHC020000000
VERSION     DAZKHC020000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.151"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            38..778
                     /locus_tag="ACHOSM_RS21725"
                     /old_locus_tag="pgaptmp_004345"
     CDS             38..778
                     /locus_tag="ACHOSM_RS21725"
                     /old_locus_tag="pgaptmp_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGU4560710.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            813..3413
                     /gene="htrE"
                     /locus_tag="ACHOSM_RS21730"
                     /old_locus_tag="pgaptmp_004346"
     CDS             813..3413
                     /gene="htrE"
                     /locus_tag="ACHOSM_RS21730"
                     /old_locus_tag="pgaptmp_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HGU4560711.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGSLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSTIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            3430..3999
                     /locus_tag="ACHOSM_RS21735"
                     /old_locus_tag="pgaptmp_004347"
     CDS             3430..3999
                     /locus_tag="ACHOSM_RS21735"
                     /old_locus_tag="pgaptmp_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4560712.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4014..4616
                     /gene="yadL"
                     /locus_tag="ACHOSM_RS21740"
                     /old_locus_tag="pgaptmp_004348"
     CDS             4014..4616
                     /gene="yadL"
                     /locus_tag="ACHOSM_RS21740"
                     /old_locus_tag="pgaptmp_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HGU4560713.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            4643..5239
                     /locus_tag="ACHOSM_RS21745"
                     /old_locus_tag="pgaptmp_004349"
     CDS             4643..5239
                     /locus_tag="ACHOSM_RS21745"
                     /old_locus_tag="pgaptmp_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGU4560714.1"
                     /translation="MHPTQRKLMKRIILFLSLQSPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            5223..6566
                     /locus_tag="ACHOSM_RS21750"
                     /old_locus_tag="pgaptmp_004350"
     CDS             5223..6566
                     /locus_tag="ACHOSM_RS21750"
                     /old_locus_tag="pgaptmp_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HGU4560715.1"
                     /translation="MCTTNNRSKQITATVTAVDGGVIMKKFFRHFLFLILCLSCYTAS
                     AGTDDNVGYIVGNSYGVGPSDQKWRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTG
                     VKLQWSQLDTASGGGFLYCNRSDSTSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYY
                     TLLISNMWSAYGTVTNVSSPGIYIGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAV
                     GGIYQTLTIEFYTDTNFDPTVTQQIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFD
                     LLNVKLTNPTCFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNI
                     ETKLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKD
                     YDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            6679..7473
                     /gene="panB"
                     /locus_tag="ACHOSM_RS21755"
                     /old_locus_tag="pgaptmp_004351"
     CDS             6679..7473
                     /gene="panB"
                     /locus_tag="ACHOSM_RS21755"
                     /old_locus_tag="pgaptmp_004351"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HGU4560716.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7485..8336
                     /gene="panC"
                     /locus_tag="ACHOSM_RS21760"
                     /old_locus_tag="pgaptmp_004352"
     CDS             7485..8336
                     /gene="panC"
                     /locus_tag="ACHOSM_RS21760"
                     /old_locus_tag="pgaptmp_004352"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HGU4560717.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(8410..>8648)
                     /gene="rpnC"
                     /locus_tag="ACHOSM_RS21765"
                     /old_locus_tag="pgaptmp_004353"
     CDS             complement(8410..>8648)
                     /gene="rpnC"
                     /locus_tag="ACHOSM_RS21765"
                     /old_locus_tag="pgaptmp_004353"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HGU4560718.1"
                     /translation="QADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQ
                     EFALRLLSKGMSREDVAEMANLPLAEIDKVINLI"
BASE COUNT         2466 a         1896 c         1965 g         2321 t
ORIGIN      
        1 tattccctgt tttaattaac tctgtcaggg atggtttatg ttttttaaca ccaaacatac
       61 aacagcttta tgcttcgtaa cctgtatggc ttttagttca tcgtctattg cggacattgt
      121 catttcgggt actcgcgtaa tatataaaag cgatcaaaaa agtgtcaacg tacgtctgga
      181 aaataaaggg aataacccgt tgcttgtcca gagttggtta gatactggcg atgacaacgc
      241 tgagcctggc agtattacag tcccttttac tgccacgccg ccagtatcgc gtattgatgc
      301 caaacgtggg caaacaatca aattaatgta cacagccagc acctcactgc ctaaagacag
      361 agagagcgtg ttctggttta acgtactgga agttccacca aaaccagatg cagaaaaggt
      421 cgcgaatcaa agtctgctgc aactggcatt tcgcacacgt ataaaacttt tctatcgccc
      481 ggatggattg aagggaaatc cctctgaagc cccgttagcc ctgaagtggt tctggtcagg
      541 ttcagaaggt aaggcgtcat tgcgagtgac caatccaacc ccttactacg tctcttttag
      601 cagtggtgat ttagaagcta gcggtaaacg ctatccgatt gatgtgaaaa tgattgcacc
      661 atttagtgat gaggtcatga aagtcactgg ccttaatggc aaagcaagtt ctgcaaaagt
      721 acatttttac gccattaatg attttggtgg cgccattgaa ggtaatgcca gtctgtaatc
      781 aggcaaggat ataattccgc aggaagcata acgtgactat aaaaccaact aaaaattatc
      841 ataatcatct gacccgtatc gctacgtttt gcgcgctgct gtattgcaat agtgctttct
      901 gtgctgaact tgttgaatat gaccatacct tcctgatggg gcagaatgcc tcaaatattg
      961 atctcagccg gtatagtgaa ggtaaccctg ctataccggg tatgtacgac gtcagtgttt
     1021 atgtcaacga tcaacctatt attaaccaaa gcattacatt tattgaaatt gaaggaaaaa
     1081 aaaacgcaca agcctgtatc acacttaaga atttattgca gtttcatata aattctcccg
     1141 atataaataa cgaaaaagcc gttctgcttg ccagggatga aacactcggc aattgcctta
     1201 atttgacgga aattatccct caggcttctg ttcgttatga cgttaacgag caacgtctgg
     1261 atatagacgt tcctcaggca tgggtaatga aaaattacca aaactatgtt gatccatcgt
     1321 tatgggaaaa tggcattaat gcggccatgt tgtcatacaa cctcaacgga tatcatagtg
     1381 aaactcctgg tcgaagaaat gacagcattt atgctgcttt taacggtggg atgaatttag
     1441 gtgcatggcg actgcgtgcc tcaggcaact acaactggat gaccgattct ggcagtaatt
     1501 atgattttaa gaatcggtat atacagcgtg atatcgcctc gctgcgttct caactcattc
     1561 ttggtgagtc ttatacaacg ggcgaaacct ttgattctgt cagtatccgc ggcattcgtt
     1621 tatacagcga cagccgcatg ttgcctccga cattagcgag ttttgcgcct atcattcatg
     1681 gcgttgctaa taccaacgcc aaagtcacta ttacgcaagg tggctataag atttatgaaa
     1741 cgactgtgcc gccaggcgct ttcgtcattg atgatctgag tccgtcaggt tacggcagcg
     1801 atcttattgt tactgtcgaa gaatccgatg gctcaaagcg gacattctcg caaccttttt
     1861 catcggtcgt tcaaatgtta cgccctggcg ttggacgttg ggatattagc ggcggccagg
     1921 tcttaaaaga tgatattcag gatgagccta atttatttca ggccagctac tactacggtc
     1981 tgaataacta tctaacgggt tatactggta ttcagattac cgataataac tataccgctg
     2041 gtttgttagg tcttggtctg aatacttcag tcggtgcatt ttctttcgat gtgactcact
     2101 ccaatgttcg tataccggat gataaaacat atcagggaca aagttatcgt gtttcctgga
     2161 acaagttatt cgaagaaaca agtacttcac tgaatatcgc ggcctatcgc tattcgacac
     2221 agaattacct tggtcttaat gatgcactaa ctctaattga tgaagtgaaa catcccgaac
     2281 aagatcttga accgaaatcc atgcgtaatt actcgcgcat gaaaaatcag gttacggtca
     2341 gtattaacca accgctaaaa tttgagaaaa aagattacgg ttcattttat ctttccggaa
     2401 gttggtccga ttactgggct tccggacaaa atcgtagcaa ttactctatt ggctacagta
     2461 acagtgcatc ttggggcagc tacagtgtca gtgcccagcg ttcatggaat gaagacggcg
     2521 acactgacga tagcgtttat ctcagtttca ccattcctat tgaaaaatta cttggcactg
     2581 aacaacggac ttcaggtttc cagagtattg atactcaaat gagcagtgac tttaagggta
     2641 ataaccaact caacgtcagt agcagtggct atagtgataa cgctcgcgtc agttatagcg
     2701 taaatactgg ctatacgatg aataaagcca gcaaagattt gagttatgtt gggggttatg
     2761 ccagctatga atcaccgtgg ggaagcctgg caggttcagt ttctgcaaat agcgataaca
     2821 gtcgtcaagt gtctctcagc accgacggtg gttttgtatt gcatagcggc ggcctgacct
     2881 tcagtaatga cagttttagc gactccgata cactggcggt agttcaggct ccaggtgctc
     2941 aaggagcgcg aataaattat ggcaacagta ctatcgatcg atggggttat ggtgtcacca
     3001 gcgctctttc tccttatcat gaaaaccgta tcgcgctgga tatcaacgat cttgagaacg
     3061 atgttgaatt aaaaagtacc agtgcagtag ctgtaccgcg tcagggttca gtcgtctttg
     3121 ctgattttga aaccgtgcaa gggcaatcaa ccattatgaa catcacacga agtgatggta
     3181 aaaatattcc atttgctgca gatatttatg atgagcaagg caatgtcatt ggtaatgttg
     3241 gacagggtgg acaagcattt gttcgtggta ttgagcagca gggaaatatc agcattaaat
     3301 ggctcgaaga aagtaaaccc gtaagttgtc ttgcgcatta tcaacaaagt tcagaagcag
     3361 aaaaaatagc gcaatctatt attctgaatg gaattcggtg tcagattcag taactacaag
     3421 ggacgtcaaa tgataaaaat aacgccacat aaaatagcga tactgatggg aatattatta
     3481 tcaccatcag tatttgcaac ggatattaat gtagagttta cagccactgt caaagcgaca
     3541 acctgtaaca tcacacttac tggtaataac gtcacgaatg atggcaataa taactacaca
     3601 ttgagaatcc ctaagatggg tctggataag atcgcgaata aaacgacaga atctcaggct
     3661 gattttaaac tggttgctag tgggtgcagc agtggcatca gttggattga taccactctg
     3721 accggaaatg catcatcaag ctcacctaag cttattatac cgcagtctgg tgattcatct
     3781 tcgacgacaa gtaatatcgg tatgggtttc aaaaaacgga ctactgatga tgccactttc
     3841 cttaaaccta acagtgcgga aaagatacgc tggagcacag acgagatgca gcccgataag
     3901 ggtcttgaaa tgaccgttgc gctacgtgaa acagatgcag ggcaaggcgt accggggaat
     3961 tttcgtgcac tggcaacgtt taatttcatc tatcaataag aaggataatt aagatgacat
     4021 ttaaaaattt acgttatgga ttatccagca gtgtcgtttt ggctgcctca ttgttcagcg
     4081 taccctctta tgcggcaaca gacagcattg ggctgaccgt gattactacc gtagaaatag
     4141 gtacttgtac cgctacatta gtaaatgact ctgatcagga catttctgtt gttgactttg
     4201 gcgatgtata tatttctgaa atcaatgcca agaccaaagt taaaacattc aaactcaaat
     4261 tcaaagactg tgcgggtatc cccaataaaa aagcacaaat aaaattaacc aagcgagcca
     4321 catgcgaagg tactgctaat gacggtgcgg ggttcgcaaa tggatccaca gccgcagata
     4381 aagcaagtgc cgtcgccgtt gaagtctgga gcaccgaaac tccggcaaca gggagcgcaa
     4441 cacaatttag ttgtgtgaca ccagcatctc aagaggtcac tatctccaat gcagccaacg
     4501 cggttgttta ttatccgatg agtgctcgac tggtcgtcga aaaaaataaa accgtaagca
     4561 atgtcactgc gggtaaattt tctgcacctg ccacatttac agtaacctat aactaactga
     4621 ctgacagatc caggaactat atatgcatcc cactcaacgt aagctgatga agagaataat
     4681 tctgtttctg tcactacagt ctcccattac ctatccagcc atcgctgggc aggatattga
     4741 ccttgttgcc aatgtaaaaa acagcacttg caaaagcgga atcagtaacc agggtaatat
     4801 tgatcttggc gtcgttgggg tgggatattt ttcagataat gttactcctg aaagctatca
     4861 accaggtgga aaagagttca ctgtcacagt atcagactgt gcattacagg gaactggcga
     4921 tgtgcttaat cagttacata ttgattttag agcccttagc ggtgtcatgg ctgcaggttc
     4981 aaggcaaata tttgccaatg aagtcgcaac cggggctaaa aatgtcggtg tcgttctctt
     5041 ttccattcag gacccgacca atatattcaa tgttattagt tcagcaggaa attctcgttc
     5101 cgtgtatcca gtaatgactt ctgccctgca taattcttca tggaaatttt atgccagaat
     5161 gcaaaaaatc gatcccgcgc tggatgtcat atctggccag gtaatgagtc atatattagt
     5221 tgatgtgtac tacgaataat agatcaaaac aaataactgc gactgttaca gcagtagatg
     5281 gtggagtgat aatgaagaaa ttcttcagac attttctttt tttaattctg tgcttgagtt
     5341 gttataccgc gagtgctggc actgatgata acgtaggata tattgttggg aatagttacg
     5401 gcgttggtcc gtcagatcaa aaatggagag aaacagggcc gaatggcgat gctactgtca
     5461 tattcagata tgcaacaagt acaaataatt tagttttcta caaaccgacg cagcttggac
     5521 ctacaggtgt aaaattacag tggagtcagt tagataccgc ttctggtggt ggttttcttt
     5581 attgcaacag aagtgatagc acaagtggta gcgcaatgcg tattgaaaat gcaatggttg
     5641 actcaggtaa aatgtatggc tcccataaat tatttaatac atcagttcct ggtttgtatt
     5701 acacattatt aatttcaaac atgtggtcag cttacggtac cgtaactaac gttagttcac
     5761 ctgggatata cattggtgac tctgcagaac aatatttttc gtggtataat ccaagcgaag
     5821 acgtgttata ttggagttgc aataatgcga atagcacccg taaatactgg gctgtaggtg
     5881 gtatttatca gacccttaca attgaattct atacagatac aaactttgat ccaacagtaa
     5941 ctcaacagat caaattatcc agctcatcaa attatctgta ttcatttaaa gcctatggcg
     6001 caggccaagg tataaatgag catagttatt ttatcaaaat cgatttcgat ctgctcaatg
     6061 tcaagttgac taatcccact tgttttaccg ctatgcttag tggaacttct gtgactggtt
     6121 ctacggtaaa aatgggtgaa tatagtgcag aacaaatcag aaacggtgcc acaccggttc
     6181 cttttgatat ttcacttcaa aactgcgttc gtgtgactaa tattgagaca aaattagttt
     6241 caacaaaggt tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgacg
     6301 cagcaaaagg agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga
     6361 cattgaaacc taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg
     6421 atacaacagg aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc
     6481 aggccacgct acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca
     6541 gtactttcca ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgcg
     6601 gtaattgata aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat
     6661 catttatcag gacacgttat gaaaccgacc accattgctt cacttcagaa atgtaagcag
     6721 gataaaaagc gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct
     6781 gatgaaggac ttaacgtcat gctggtaggc gactcgctgg gcatgacggt tcagggtcac
     6841 gactccaccc tgccggtgac cgttgccgat atcgcctacc acactgcctc cgtacgtcgt
     6901 ggtgcgccaa actgcctgct gttggctgac ctgccgttta tggcgtatgc cacgccggaa
     6961 caagcctttg aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag
     7021 ggcggtgagt ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt
     7081 ggtcacttag gtttaacccc acagtcagtg aatattttcg gtggctacaa agttcagggg
     7141 cgcggcgatg aagcgggcga tcaactgctc agcgatgcat tagccttaga agccgctggg
     7201 gcacagctgc tggtgctgga atgcgtgccg gttgaactgg caaaacgtat taccgaagca
     7261 ctggcgatcc cggttattgg cattggcgca ggcaacgtca ctgacgggca gatcctcgtg
     7321 atgcacgacg ccttcggcat taccggcggt cacattccta aattcgctaa aaatttcctc
     7381 gccgaaacgg gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc
     7441 gtttatccgg gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa
     7501 ccctgccgct gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc
     7561 tggtgcctac tatgggtaat ctgcacgatg gccatatgaa gctggtcgac gaagccaaag
     7621 cccgcgccga tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg
     7681 aagatctggc acgttatcca cggaccttgc aggaggactg cgagaagcta aacaaacgta
     7741 aagtggattt agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc
     7801 acacttacgt tgacgttcct ggcctttcga ccatgctgga aggtgccagc cgtccgggac
     7861 attttcgcgg cgtttcgact attgtcagca agctgttcaa cctggtccag ccggacatcg
     7921 cctgcttcgg tgaaaaagat tttcagcaac tggcgctgat ccgcaaaatg gttgccgata
     7981 tgggcttcga tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc
     8041 taagttcccg taacggttat ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca
     8101 aagttttaag ttcgattgct gacaaattgc aggctgggga acgggatctc gatgaaatta
     8161 ttaccattgc ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc
     8221 gcgatgccga cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag
     8281 ccgcctggct tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact
     8341 taactggcgc tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac
     8401 acaggtaact taaataaggt taattacctt atcaatctca gcaagaggta aatttgccat
     8461 ctctgcaacg tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct
     8521 ttcctcctgt cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc
     8581 cagcgtcatc atagactccc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc
     8641 cgcttgtt
//
LOCUS       DAZKHC020000152         8638 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZKHC020000152 DAZKHC020000000
VERSION     DAZKHC020000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.152"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1818
                     /gene="espL4"
                     /locus_tag="ACHOSM_RS21770"
                     /old_locus_tag="pgaptmp_004354"
     CDS             <1..1818
                     /gene="espL4"
                     /locus_tag="ACHOSM_RS21770"
                     /old_locus_tag="pgaptmp_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HGU4560719.1"
                     /translation="NLKPDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAAQYINDIY
                     QNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFGLVMKAYFD
                     KMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKIRIMSESK
                     EDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLILKGSVDEITPPLSP
                     ATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSINLPGLYLAINYGNADIVE
                     TIFNSLSETGYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKDKNVVTSILNVLPKLA
                     ATHHLDNEQVYKFLSAKNRTSSHVLYHVMANGDADMLKIVLDALPLLIRTCHLTKEQV
                     LDLLKPKDFYGCPGLYLAMQNGHSDIVKVILEALPSLAQEINISASDIVDLLTAKSLA
                     RDTGLFMAMQRGHMNVINTIFNALPTLFNTFKFDKKNMKPLLLANNSNEYPGLFSAIQ
                     HKQQNVVETVYLALSDHARLFGFTAEDIMDFWQHKAPQKYSASELTFELGHRVIAELI
                     LNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(1787..3523)
                     /gene="aceK"
                     /locus_tag="ACHOSM_RS21775"
                     /old_locus_tag="pgaptmp_004355"
     CDS             complement(1787..3523)
                     /gene="aceK"
                     /locus_tag="ACHOSM_RS21775"
                     /old_locus_tag="pgaptmp_004355"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HGU4560720.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLHVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDINYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3804..5108)
                     /gene="aceA"
                     /locus_tag="ACHOSM_RS21780"
                     /old_locus_tag="pgaptmp_004356"
     CDS             complement(3804..5108)
                     /gene="aceA"
                     /locus_tag="ACHOSM_RS21780"
                     /old_locus_tag="pgaptmp_004356"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HGU4560721.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAKGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5138..6739)
                     /gene="aceB"
                     /locus_tag="ACHOSM_RS21785"
                     /old_locus_tag="pgaptmp_004357"
     CDS             complement(5138..6739)
                     /gene="aceB"
                     /locus_tag="ACHOSM_RS21785"
                     /old_locus_tag="pgaptmp_004357"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HGU4560722.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(7008..7937)
                     /gene="metA"
                     /locus_tag="ACHOSM_RS21790"
                     /old_locus_tag="pgaptmp_004358"
     CDS             complement(7008..7937)
                     /gene="metA"
                     /locus_tag="ACHOSM_RS21790"
                     /old_locus_tag="pgaptmp_004358"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGU4560723.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8094..8537
                     /locus_tag="ACHOSM_RS21795"
                     /old_locus_tag="pgaptmp_004359"
     CDS             8094..8537
                     /locus_tag="ACHOSM_RS21795"
                     /old_locus_tag="pgaptmp_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU4560724.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2200 a         2125 c         2141 g         2172 t
ORIGIN      
        1 aacctaaaac cagatagaaa agaaaaagaa ttcccaagaa ttaaacttaa tggtcaatgt
       61 tattttccgg ggcgacccca aaaccgtata gtatgccgac acattgctgc acaatatatt
      121 aatgatattt atcagaatgt tgattacaaa ccccatcaag atgattattc ttcagctgaa
      181 aaatttctca ctcacttcaa caaaaaatgc aaaaaccaga ctttggcgtt gatttccagc
      241 cgtcctgagg ggcgttgcgt tgctgcctgc ggtgatttcg ggctagttat gaaagcatat
      301 tttgacaaga tggaatcaaa tggcatcagt gttatggcag ccatattact ggtggataac
      361 catgctttga cggtccggct aagaataaag aacacaactg aaggatgtac ccattacgtg
      421 gtttcggttt atgatcctaa tgtaactaac gataaaataa gaatcatgag cgaaagcaaa
      481 gaggatatta aacactattc tctgatggat tttatgaatg tagattatag cctcctgaaa
      541 tggtcaaatg atcatgttat taaccaatct gttgcaataa ttccagcact tccgaaagaa
      601 cagctattga tattaaaagg atctgtggat gaaataaccc ctccattatc accagcaacg
      661 atgaatttgc taatggcaat tggtcagaat caccaactta cgcaactgat gattcagctc
      721 cagaaaatgc cagaactaca tagaacagaa atgttgactg cctataatag tattaacctc
      781 cccggtttat atttggctat aaattatggc aatgcggata tcgttgagac tattttcaat
      841 tcattgtcag aaacaggata tgaagggtta ctctcgaaaa aaaatctcat gcatattctg
      901 gaggcaaaag ataaaaatgg tttttctgga ttatttttag cgatatcacg taaggataaa
      961 aatgttgtaa cctcgattct gaacgtctta cctaaactgg ccgcaacaca tcatttagat
     1021 aatgaacagg tgtataaatt cctgagtgcc aaaaatagaa cgtccagcca tgttttatac
     1081 catgttatgg cgaatggtga tgccgacatg ctgaaaattg ttttggacgc gttacctttg
     1141 ttaattcgca catgtcattt gactaaagaa caggtactcg atctcctgaa gccaaaggat
     1201 ttttatggtt gcccaggact atacctggcg atgcaaaatg gacatagcga tatcgtgaaa
     1261 gttattcttg aagcattgcc cagcctggcc caggaaatta acatttcagc ttccgatatt
     1321 gtagatcttc tgaccgctaa aagtcttgcg cgcgacacgg gtttgtttat ggccatgcag
     1381 cgcggacata tgaacgttat taatactatt tttaacgcat tacccactct gtttaatacg
     1441 tttaaattcg ataaaaaaaa tatgaagccc ctcctcctgg caaataattc taatgaatat
     1501 cccggtttgt tttcagcgat acagcataaa caacaaaatg ttgtagagac ggtttatctt
     1561 gctttatctg accatgcacg cctgtttgga tttactgctg aagatattat ggatttttgg
     1621 caacacaaag cgccacaaaa atactctgcc tctgagttga cttttgaatt gggtcaccgg
     1681 gttattgctg aattaatcct taatacatta aataagatgg ctgaaagctt tggctttacg
     1741 gataaccctc gatacattgc ggagaaaaat tatatggaag ctttactcaa aaaagcatct
     1801 ccccataccg tacgctaaat ctttgcctgc gccgatacgc ataaacatct tccacatgcc
     1861 cttcacgtat gcggttttgt agtgcgcgcc agtaatcagc gcggaacagg tcggcgtgca
     1921 tctcttcaaa cagcggacca atacgcgggt cggcgcatag ccagtggcga aactcttccg
     1981 ggaaaacatc gcccggcgag acgctgtacc acggttcgct ggcaagttcg tcttccggat
     2041 agcgcggcgg cgggatgtcg cggaaattca cttccgtcat gtagcaaatt tcatcgtaat
     2101 cataaaaaac cacacgcccg tgacgggtga caccgaagtt tttaaacagc atgtcgccag
     2161 ggaaaatgtt agcagcggca agctggcgaa tagcgttacc gtattcttca atggcgtcgc
     2221 gcaactgctg accttccact tgttccagcc agatattgag cggcaccatc cgccgctcga
     2281 tatagagatg gcgaattaca atctgctcac caagatcggt gattttttcc gctgcttcct
     2341 ggagcagtaa ttccattaat gccggggaaa tatgccgctt ctccagcaca aagttttcaa
     2401 actcctgggt gtccgccatt cggcccacac ggtcgtgctc tttcaccagt tgatagcagg
     2461 cacgaacgtg agcggcagac atctctttct gcggcgcgaa cttgtctttg atgactttga
     2521 atacccgatc aaagcccggc agcgtaaaca ccaacatcac cattccacga atacccggcg
     2581 cttcaatgaa ctgctcatta cagccctgta gataaacgag atattcgcgg tagctttcgg
     2641 ttttggcgtg cttctggcag ccgatagcca tatacaattc agcagtggtt ttacctggca
     2701 gaatttcccg tagccactcg accagcgctg cgggcagcgg tgcataaacc ataaaataag
     2761 aacgcgcaaa gccaaaaaca atgctcgctt cggcggtcgt cgtcaggcag gtatcaataa
     2821 ataactcgcc gtcgtccgtc tggtggatcg gcagtaagaa gggcaacgtg ccggaaggcg
     2881 tgatcagttt gcccaccagc caggcggctt tattgcggta aaacagttcg ttcgccacct
     2941 gtaaatgact ttccgcgagg ttgtctgtcc ccagcgtttc cgtcagatgg cgaataatgt
     3001 aattgatatc acggctttta ttctgccagc gcaggcgcag cggcaggtcg ctgataacgc
     3061 gcatcagcag agattcccag ccgtgatcgg ggtgaaagtc tttcgccagc gggcggggaa
     3121 tggtacgaaa gcggcgctct ggctgggagc taaagataaa aagccgctcg ggagtaagcg
     3181 agcggtggtc aaataaccga cagtacacgg agttaaaaaa gctctccgca atctcgaagc
     3241 gcgggtaatc cggcaacagc cgcgtgtaat gctctttaac gtgcagtaaa aatgccgcgt
     3301 ccgtactttg gccgttagta atgcagcgca gttgctccac gaccagacca acgtgatgat
     3361 cgtaaagatg gatacggttt ttcatcgcct gctggacagc atgccagtcg gcctgttcga
     3421 aacgctgctg cgccccggaa gtcacttcga ggaatcgacc atactgagcg tcgaagcctt
     3481 gcaaaatggt ttgtgcaatc agtaattcca ggccacgcgg catttagcgc cctcatcagg
     3541 agcagagaat tgccggatgc ggcgtgaacg ccttatccag cctacgttcg gcaacggttg
     3601 taggcatgat aagacgcgca agcgtcgcat caggcatcgt gcaccgattg ccggatgcgg
     3661 cgtaaatgcc ttatccggcc tacgttcggc aacggctgta ggcatgataa gacgcgcaag
     3721 cgtcgcatca ggcatcgtgc accgattgcc ggatgcggcg tgaacgcctt atccggccta
     3781 cagtcagcaa cggttgttgt tgcttagaac tgcgattctt cggtggagcc ggtcagcgct
     3841 gtaaccgacg acgtgccgcc ctgaataata gtcgtcactt tatcgaagta acctgtaccc
     3901 acttcctgct ggtgagatac gaaggtatag ccatctttcg cggcggcaaa ttccggctgc
     3961 tgcactttct caacgtagtg cttcataccc tcgcccttgg cataggcgtt tgccaggtca
     4021 aacatgttga accacatgct gtggatacct gccagggtga tgaactggaa cttgtagccc
     4081 atatccgaca gctgctgctg gaagctggca atagttttgt cgtcgaggtt tttctgccag
     4141 ttgaacgacg gcgagcagtt ataagccagc agtttgcccg gatatttcgc gtggatagct
     4201 tgtgcaaagc gacgcgccag ttccagatcc ggcgtggagg tttcacacca gaccaggtca
     4261 gcatatggcg catacgccag gccacggctg atcgcttgct caatgcccgc atgagtacgg
     4321 aagaagcctt cactggtacg ctcgccggta ataaattcgc tgtcatacgg gtcgcaatcg
     4381 gaggtgatca gatccgctgc atcggcatcg gtacgagcaa ccagtagggt tggaacgccc
     4441 gtcacgtcag ctgccagacg cgccgcgacc agtttctgaa tagcttcttg agttggcacc
     4501 aaaactttgc cgcccatgtg accgcatttc ttcactgacg ccagctgatc ttcgaagtga
     4561 actgccgctg caccggcttc aatcatcgct ttcatcagtt caaaggcatt caggacaccg
     4621 ccaaaaccgg cttccgcatc ggcaacgatc ggcaggaagt aatccacata gcgcggatcg
     4681 cccggctcaa tgcccgcgga ccattggatc tgatccgcac ggcggaaagt attgttgatc
     4741 cgctccacca cagctggcac cgagtttgcc ggatagagcg actgatccgg atacatgctg
     4801 gccgccaggt tagcgtccgc cgctacctgc catcccgaca gatagactgc ttcaataccc
     4861 gctttcgcct gttgcagcgc ctgaccgcca gtcagtgcac cgaggctgtt gatgtagcct
     4921 tttttcgact cgccgtgcag cagacgccac attttcgccg cgcccagttg cgccagcgtg
     4981 cattcaggat tgactgaacc gcgtaatttc accacatctt ccgcgctgta tgggcgagta
     5041 atgccttccc aacgcggttg agtccactct ttctgtaatt cttcaatttg ttgtgtacgg
     5101 gttttcatgt gcagatgctc catagttatg tggtggttta cgctaacagg cggtagcctg
     5161 gcagggtcag gaaatcaatt aactcatcgg aagtggtgat ctgttccatc aagcgtgcgg
     5221 catcgtcaaa acggccctgg gagaaacgtt cttcgcccag ttcgctggca atgactttca
     5281 tctcttcgcc cagcatctgg cggaacaagg ctttggtcac cggtttgcca ttgctcaacg
     5341 ttttttgatg atggatccac tgccagatcg aggtacggga aatttcagcc gtcgccgcat
     5401 cttccatcag gccataaatc ggcacgcagc cgttgccgga gatccacgct tcgatgtact
     5461 gcacagccac gcgaatgttg gcgcgcatac cttcttcggt gcgttcacca tcacaaggtg
     5521 ccagcagctg atcggcagta atcggcgcgt cttgttcgcg caatacttca agctgatttt
     5581 tacgggagcc gagaatgtcg ttgaataccg ccattgccgt atccgcaagg cctgggtgag
     5641 cgatccatgt gccatcgtga ccgttattgg cttccagaga tttatccgct tttactttgt
     5701 tgagcacctg gttattgtgc tcttcatctt tgctcggaat aaacgccgcc atgccgccca
     5761 tcgcaaaagc accgcgttta tggcaggttt taatcaacag gcgtgagtaa gcattcagga
     5821 agggtttatc catcgtcact gcctgtctgt ctggcaggac gcgatcggga tagtttttca
     5881 acgttttgat atagctgaag atgtaatccc aacgaccgca gttcagacca acaatatggt
     5941 cacgcagcgc gtgaaggatt tcatccatct ggaacacggc gggcagcgtt tcaatcagca
     6001 acgtcgcctt gatggtgccg cgcggcagat taaagcgatc ttctgcatag ctgaagactt
     6061 cgctccacca ggccgcttcc tgccaggact gggttttcgg cagatagaaa tagggaccgc
     6121 tgccctttgc caacagtgcc tgatagttgt ggaagaaata gagcgcaaaa tcaaacaggc
     6181 taccggggat tgcctcacca cgccaggtga catgtttttc cggcaagtgc agaccgcgta
     6241 cccgacaaat caaaaccgct ggattgggct tgagctgata aattttgcct gcttcattgg
     6301 tatagctgat ggtgccgtta accgcatcgc gcaggttaat ttgcccgtcg atcactttgt
     6361 tccagtctgg tgccagtgaa tcttcgaaat cggccataaa gactttcaca ttggcgttca
     6421 gtgcgttgat caccatcttg cgctcaaccg ggccagttat ctcgactcga cgatcttgta
     6481 agtccgcggg aataccacga attttccagt ccgtatcgcg aatggaagct gtttccgaaa
     6541 taaaatcagg caacgttccg ttatcaatat cttgctgctg ctgaatgcgc gctgccagaa
     6601 gtttattgcg ttgtggcgta aaatgcgtca ccagctcagt cagaaattct accgcttcgg
     6661 cagtaagaat ttgcttctcc tgctcgccat acggccttat gaaagccagt tcatcggttg
     6721 ttgttgcctg ttcagtcatc gtgcagctcc tcgtcatgga tccgaaaact tccccactgt
     6781 gaacgaagga tcgttgtgcg cttttcgttc agcacaacta agactactca tttaaaatgc
     6841 aaaatcaaaa acaatttcca ttttaattaa aaatacttga taacttattg ataacaatat
     6901 gaataaaatt tattcacctc ctgaggtgcg tttcggaaat aaaaaaggca cccgaaggtg
     6961 cctgaggtaa ggtgctgaat cgcttaacga tcgactatca cagaagatta atccagcgtt
     7021 ggattcatgt gccgtagatc gtatggcgtg atctgataga cgtaatagtt gagccagttg
     7081 gtaaacagca aattaccgtg actacgccag ctcgctcgcg gtgtattttg cggatcattg
     7141 tgcgggaaat agttatacgg tacttccggg cctagtccgg cttccacatc gcggaaatat
     7201 tcctgcgcca gcgtttgcgc atcatattcg ggatggcccg tcacaaaggc aatgcgctta
     7261 tctttgctgg caaacagata tgcatcccct tcttccgtct ctgccagaat ttccagatcg
     7321 gtgtaatcac gaatcaacgc tgccggaaag tcagcatagc gcgaatgtgg tgccaggaat
     7381 gaatcatcaa agccacgcgt cagaagcgca tgaggatgga gaatatgatg ctcgtaaacg
     7441 ccagagagtt tgtcggtgcg agtttgctta ggaatgccgt agaggatatt gagcgcggcc
     7501 tgtaccgccc agcagacaaa cagcgtcgag gtgacgtgat ctttcgacca ctccagcacc
     7561 tgtttgatct gcggccagta agcgacatca ttaaactcca ccaggcccag cggcgcaccg
     7621 gttacaatca gaccgtcaaa gttctgttcc tgaatatctt caaagttaca gtagaagttg
     7681 ttcagatgct ctgcgggcgt gttgcgcgat tcacgggaat cgatgcgcaa cagctgaata
     7741 tcgacctgca aaggtgagtt tgaaagcagg cgcagaaact gattttcagt ttcaatcttc
     7801 ttcggcatca ggttaaggat cagaacctta agtggacgaa tttcctgacc agacgcacga
     7861 gaagttgtca tcacaaagac gttttcttca cgcaagaaat tgacggcggg tagctcgtcc
     7921 ggcacacgaa tcggcataac ctgattacct cactacatac gcttatacgt ttagacatcc
     7981 agatagctga agataaccag aaaatttgcc aatgtcgagc ctgcatgttg aaggtgagaa
     8041 agtttcaagg aaaaggctac gttagaatat aagaatgacg aaaaggagag aaaatggtta
     8101 ttagtattcg ccgctcacgg catgaggaag gggaggaact cgttgcgatt tggtgtcgtt
     8161 ctgtcgatgc cacacacgat tttttatcag cagagtatcg ggccgagctg gaagtgctag
     8221 ttcgttcctt cctgccggaa gcgccgttgt gggtcgcggt taatgaacgg gatcagccgg
     8281 ttgggtttat gttgttaagt gggcagcata tggatgcgct gtttatcgat cctgatgtgt
     8341 gcggctgcgg cgtgggtcgg atgctggtgg agcatgcgct ctcgatggca ccggaactga
     8401 caaccaacgt taatgagcaa aatgagcagg cggttgggtt ctataagaag gtgggtttta
     8461 aggttacggg acgctctgag gtggacgatt tggggaaacc gtatccgttg ctgaatctgg
     8521 cgtatgtggg ggaataagcc gcccctgccg tgcctgccca ggctggagaa gacgcgccag
     8581 cgtcgcaccc ggcatttttt tcaccaacgc aaaaaggcca tcctgacgga tggccttt
//
LOCUS       DAZKHC020000153         8606 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZKHC020000153 DAZKHC020000000
VERSION     DAZKHC020000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.153"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..126)
                     /locus_tag="ACHOSM_RS21800"
                     /old_locus_tag="pgaptmp_004360"
     CDS             complement(<1..126)
                     /locus_tag="ACHOSM_RS21800"
                     /old_locus_tag="pgaptmp_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560725.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWP"
     gene            204..1298
                     /gene="ddlA"
                     /locus_tag="ACHOSM_RS21805"
                     /old_locus_tag="pgaptmp_004361"
     CDS             204..1298
                     /gene="ddlA"
                     /locus_tag="ACHOSM_RS21805"
                     /old_locus_tag="pgaptmp_004361"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU4560726.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1322..1534)
                     /gene="yaiZ"
                     /locus_tag="ACHOSM_RS21810"
                     /old_locus_tag="pgaptmp_004362"
     CDS             complement(1322..1534)
                     /gene="yaiZ"
                     /locus_tag="ACHOSM_RS21810"
                     /old_locus_tag="pgaptmp_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HGU4560727.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1794..2102
                     /gene="yaiY"
                     /locus_tag="ACHOSM_RS21815"
                     /old_locus_tag="pgaptmp_004363"
     CDS             1794..2102
                     /gene="yaiY"
                     /locus_tag="ACHOSM_RS21815"
                     /old_locus_tag="pgaptmp_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HGU4560728.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2161..3255)
                     /gene="yaiW"
                     /locus_tag="ACHOSM_RS21820"
                     /old_locus_tag="pgaptmp_004364"
     CDS             complement(2161..3255)
                     /gene="yaiW"
                     /locus_tag="ACHOSM_RS21820"
                     /old_locus_tag="pgaptmp_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HGU4560729.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3268..4488)
                     /gene="sbmA"
                     /locus_tag="ACHOSM_RS21825"
                     /old_locus_tag="pgaptmp_004365"
     CDS             complement(3268..4488)
                     /gene="sbmA"
                     /locus_tag="ACHOSM_RS21825"
                     /old_locus_tag="pgaptmp_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HGU4560730.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4840..5997
                     /gene="ampH"
                     /locus_tag="ACHOSM_RS21830"
                     /old_locus_tag="pgaptmp_004366"
     CDS             4840..5997
                     /gene="ampH"
                     /locus_tag="ACHOSM_RS21830"
                     /old_locus_tag="pgaptmp_004366"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HGU4560731.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5998..6621)
                     /gene="iprA"
                     /locus_tag="ACHOSM_RS21835"
                     /old_locus_tag="pgaptmp_004367"
     CDS             complement(5998..6621)
                     /gene="iprA"
                     /locus_tag="ACHOSM_RS21835"
                     /old_locus_tag="pgaptmp_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HGU4560732.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWHNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6709..>8606)
                     /locus_tag="ACHOSM_RS21840"
                     /old_locus_tag="pgaptmp_004368"
     CDS             complement(6709..>8606)
                     /locus_tag="ACHOSM_RS21840"
                     /old_locus_tag="pgaptmp_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4560733.1"
                     /translation="DDAQNVDELNITLNNGSKWVGSANMSAEVIAPADMYDVAPNSLT
                     PGATIEANDWGRIIDNKVFQSGVFNVALNNSSEWNTVNSSVIDTLAVNNGSQVNVTDS
                     SLVSDTIGLTNGSSLNIGANGVVATDHLTVDSYSTVNLTESTGWNNYSNLYTNTITVT
                     NGGVLDVNVDQFDTEAFRTDKLELTSGNIADHNGNVISGVFDINSSDYVLNADLVNDR
                     TWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNGTELDNSSVDNVAAATGNYKVRIDNA
                     TGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDY
                     ANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYD
                     QDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFAN
                     NVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYG
                     SVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVY
                     DDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSAN
                     VGVKYTW"
BASE COUNT         2181 a         2181 c         2072 g         2172 t
ORIGIN      
        1 tggccattta agccaagaat aaattagctt cccgttcatc atttttgtga tcttgcgcac
       61 atctttgaga taacgcactt tcttcaccag tacgttgcac gctttgtcag cagaagattg
      121 caacaatctt tcagaaagcc gcgaatcccc cgttttgtcg tggtaatgta tgcactttgt
      181 gttagacggg ataggttttt aagatggaaa aactgcgggt aggaatcgtt tttggtggta
      241 aatcagcgga acatgaagtg tctctgcagt cggcaaaaaa cattgtcgat gccattgata
      301 aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca agggcaatgg cacgtcagcg
      361 atgccagcaa ttatctgcta aatgcagacg atcctgccca tattgcgttg cgcccttcgg
      421 cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca gcttatcgac gcgcaaaacg
      481 gtcagccgtt gccgacggtg gatgtcattt tcccgattgt ccacggtacg ctgggcgaag
      541 atggttcctt gcagggaatg ctgcgggtcg ccaatttacc gtttgtaggt tctgatgttc
      601 tggcttcagc agcctgtatg gataaagatg ttaccaaacg tctgctgcgc gatgccgggc
      661 tgaacattgc gccatttatt accctgacgc gcgccaatcg tcacaacatt agttttgccg
      721 aagtggagtc taaactgggg ttaccactgt ttgtaaaacc ggctaatcag ggctcttctg
      781 ttggtgtcag caaagtaacc agtgaagaac agtacgcaat tgccgtcgat ctggcgttcg
      841 aattcgatca taaagtgatc gttgagcaag ggatcaaagg tcgtgagatc gaatgcgcag
      901 ttctgggcaa cgacaacccg caagccagca cctgtggcga gatcgtactc accagcgact
      961 tctatgccta cgacaccaag tacattgacg aagatggcgc gaaagtggta gttccggcag
     1021 ccattgcgcc agaaatcaac gataagatcc gggcgattgc cgttcaggct tatcaaacgt
     1081 tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac cccagagaac gaagtggtga
     1141 tcaacgagat caacacactg cctggcttca ccaacatcag tatgtatccg aagctgtggc
     1201 aagccagcgg tctcggttac accgatctga tcacacgttt gattgaactg gcgctggagc
     1261 gtcacgctgc ggataacgca ttgaaaacca caatgtaata ttttaacgcc cgataatatt
     1321 cttattgggc gttttcagtt tcttcatcgc gacgacgaat aatcagcccc gccagccaga
     1381 aactaatcac ccacgtcacc agaccgacgg cataggtctg ccaacctttt gcttcaaatc
     1441 ccagtagccc caccacgcca ttaagaatga atataaggcc aatggcgaac gcatagtagt
     1501 gccagtcacg gcggattttt acaggcagat tcatggctgc tccgttaacg aaaaacaaca
     1561 tccttgcata ttctgcaaca accgtctgtg gtgtagacaa cactttcgca attgttaata
     1621 aatttaactt aaagttgctg ttttgtgact cgcaacgaag attcacgctt cgggaatatt
     1681 cccaacatca atttaccatg attctgatat tgacaatcta aatgacctgt cagacttgcc
     1741 ataagccgct ggtatcacac cagaagagaa gcagatgact tccggaggtt tttatggctg
     1801 atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct tcaacgcctg
     1861 gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg ggctggggcg caattgctga
     1921 acctgttagt gcatgcgccc ggcgtctatg agcgtttaat gcaagtccag gaaacaggtc
     1981 gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt cgggctgatc ccgtttttag
     2041 taggctgcct catttttgca gtggtggcgc tatggctgca ctggcgacat cgccgccagt
     2101 aagttttacg ttgtcagatg cggcctgaaa cgccttatct ggcatgacat tcctgtttga
     2161 tcactgtttc atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc aggccgtagt
     2221 caggttgcgg gtgattttcg ggctttccag ttgaatgcca ggcaacattt cgcgggggag
     2281 tgatttaccc gttttcgctt cggcaagttg ataaactttc ttgtacagcg ccgtctcctc
     2341 aaaagagaag ctgtcacctt tctctaactg acggcgaatt tcgctgtcgc tcatccccag
     2401 ttttgccgcc agtttgcgcg ttgccagttc ggttttcccg ggttctttac tgtcgtaacg
     2461 aattaaatca ccgtccagtg ccagcttcac gccgctggcc ttactgaccg cgttctgaaa
     2521 cgcggcatta cggctggcgt accagccagc gttaaaatca gcaaagcggt atatcggtgc
     2581 gctatagctg gcgggataat tcagtaaatg gtaagtacca aaccacaacc cgccgcggcg
     2641 actgaaaact tcctgacgaa ctgtaccgtc cattttccac ggataccctt tggtatgctg
     2701 ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg cgcacaggat taagcgaacc
     2761 aaacagcgtc tgccccattg gcaccatgct gatcagatcg tcaaaaatcg cgctcaattg
     2821 cttttctgta cgcaccgaat ctaaccgttc gctataactt ttgccgtttg gcgatttgat
     2881 ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc cgttcagcac gacggtcaat
     2941 ctcttgccag gcgatcttgc ttaagcccgg aacggccgga tctgcctgat aatttgactc
     3001 ctgctgcgcc accgccagca ccgagcagac gttttccagc gtcggtgcca gcccctgact
     3061 ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg tctttcacgc tcgcaggcat
     3121 cttctgccgc acgacgctcg ctacatctat cgctttttcg ccctttttca acggttgcgg
     3181 agcctgacta ctacaccccg ctaaaaccag aaccgccaac aatgacagcg aagatagatg
     3241 attgacgcgc gacattactt ctccttttta gctcaaggta tgggttactt cctgaatttt
     3301 gtcaccatcc agctcatgtt caaagctgcg cagacgtttg tagatagaca tcaactcaac
     3361 cagtgtggtc catgagttaa tcaggtactg gaaagcaccg cgaacctgac caaaaacgtt
     3421 ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca acaattgacg gaaacagcaa
     3481 gaacaaaccg aaaacgttat cgacctgcaa atagagaatg cgggcgatgt tgaaatacat
     3541 atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg ctaaacagct cgcgtaccgt
     3601 aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc agctctttac ggtaggcggc
     3661 ctctactcgc tggtttttaa actccagccc cggcagtttg atccctacca ctgccagcaa
     3721 tccggtcccc atcagcgacc agacgatagc ggcaatcacc agaccatacg gaatgtgccc
     3781 gataatcggc agctccggca catgcgcgga gagcgttacc agcaccggca ggaaggcgat
     3841 caacgtcatg atggcgttga taaaactgac gcccatattc tccagcgttg aagcaaaacg
     3901 catggtgtct tcctgcacac gctgtgcggc accttcgata tgacgcagtt gttgccagtt
     3961 cgccatataa tattcgttca tcgccgtacg ccagcggaac acatagtgac tgacaaagaa
     4021 gttgttcagt acactgatca ccaccgcgat cagcgcaatc cccagaaaga cgcccacttc
     4081 gcggtaaaat tgttcgatag tgactttatg cggcgaactt agcgcggttt gaatcagatc
     4141 atagaacggt gcataccagg cgttgacggc gaccccgact tccaccaaaa accaggtgac
     4201 gaagatgatc agtgcagtac cgagtattga ccagtattgc caacggtgcg ggctgtagat
     4261 aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa aaatcaggaa
     4321 atccaacgac cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc cggtgatacg
     4381 cgccacccag tctcccccac cggcttgcca gaagataacg gcaatcaatg cccaaacaaa
     4441 ggccgagaga aaaaacgctc ccggctttgg gaaaaaagac ttaaacatat gcactcctgc
     4501 taacttctta tcgttttaac aactgctgtg tattaaccgc atgaccacta caaagcgtca
     4561 gccaaatgaa accgcccatt gtgacccata gcaaggcgct tagttcgcca gcctgaccat
     4621 aagaattgtt tcgacggatt tcacaaacca aagattgttt acataagcgg attaacacgc
     4681 gcaggatcac aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac gcgccgttac
     4741 gtttttaaca ctattattgc actaattcgc cctttgcaat ccatcaatga gtagtataaa
     4801 tacgctcagt taccttcatt caatctatgg acaccaccgt tgaaacgtag tctgcttttt
     4861 tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg cggcgcagcc gatcactgaa
     4921 ccggagttcg cctctgatat tgttgatcgt tatgccgatc atatttttta cggcagcggt
     4981 gccacgggga tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg cagttatggc
     5041 gaaacgcgac ctggtaataa cgttcgcccg cagctggatt cggtcgtgcg tattgcttcc
     5101 ctcaccaagc taatgaccag tgagatgctg gtgaaattgc tcgaccaggg gaccgtgaag
     5161 ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac gcgtgccaac ctacaatggg
     5221 acgcccatta cgctggtcaa tctggcaacc cataccagcg cgctgccccg tgaacagccc
     5281 ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc gcgagcaacg ctggaaatac
     5341 ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg cggcgtactc taaccttgcg
     5401 ttcgacctgc tggctgatgc actggcgaat gcgtccggca agccttatac ccagttgttt
     5461 gaagagcaga ttacccgtcc attggggatg aaagacacca cctacacccc ctcaccggat
     5521 cagtgccgcc gtttaatggt tgcagagcgc ggtgccagtc catgcaataa cacgctggcg
     5581 gcaattggca gcggcggcgt ctattccacg cctggcgata tgatgcgctg gatgcagcag
     5641 tatttgtcat ctgattttta tcagcgcagt aaccaggcag atcgcatgca gacgttgatt
     5701 tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg tccccggcaa agcggatgcg
     5761 cttggtttag gctgggtata tatggccccg aaagagggtc gtccggggat tattcagaag
     5821 acaggaggtg gtggcggttt cattacctat atggcgatga tcccgcagaa gaatattggc
     5881 gcgtttgtgg ttgtcactcg ttcgccgttg acgcgcttta aaaatatgag cgatggcatc
     5941 aatgacctgg tgaccgagct aagcgggaat aaaccgctgg ttatccccgc gtcctgatta
     6001 atactctgaa ggcaaacggt tgatagcgac cagtttgcct ttattcattt cgatatagcc
     6061 gcctttacgc aaagcagcga gaacttctgc gacgacagaa cgcgatatgc gtgtacgttg
     6121 atggatataa ttcatcacac caatacgtga gcgcaattct tcattccagt caatcattga
     6181 taataatgtt gcgcggattt gttcgtagga attatgccca atgagctgca cgtcgcgtaa
     6241 ttccagaatt ctattatgcc aggctaacca gtaaaaagcg tctcgccaga gttgattttg
     6301 ttcaataagc gtaatggttt gtttggctgg tagatgatat cccgtacaat ttccttctga
     6361 tattaatttg tatggtatat cgtttttcat taaaccatca gccagcccca taatataagg
     6421 agcctgggta ataccgataa gtacgttttc ttctctacgc agagagataa cgccctctaa
     6481 aataacgaaa gtatcttcgc tattgacatc actggaaaat ataacttgct tttcattatt
     6541 aaactcgaag cgcgtaccgt atctggacaa acatttatcg agcttaccaa attcctgaag
     6601 aggtttaact acagataaca tttgcgcgtc ctttgcagta atgcccgtca aatccttgac
     6661 gggcattatt tagattaaat taccagaatt tcttcggagt gaagaatatt accaagtata
     6721 tttaacaccc acgttcgcgg accagtcttg atctacgtca ccaccaccga ggtagttagc
     6781 atcggtatag gcgctgaagt tcttggtgaa gctgaactga gtacccagac caacacgtac
     6841 cgcagaccct tcagtaccgt tatcgatgga atcaccgttc acatcgttat cgttgttaga
     6901 gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga gcctggtctt cgctgtaggt
     6961 gaaggtataa cctgcatcta cacccagttc ataacgcatg ctgtcgtaag actgaccgtc
     7021 aactttcatg tcgttgctca gctggtagtc atcaccagac tggaacagac cagaaacgct
     7081 gccgtaagga gtcacgtaac cagcatcacc cagtttgaag tcgtaaccgg ctttcaaacc
     7141 gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa gtaccgttgc tcatggttgc
     7201 agacaggtcg ttgttgaagt gagagtagct caagctacca tcaacaaaga cgttgttcgc
     7261 gaagtgagca gaagagtaga tgtaggcagt ctggctgtct tgatccacct gaccagaacg
     7321 gtcattcatg tcacctttag cgaagcctgc agccgcaccg acgatccact tagcgttgtt
     7381 accgtcaatt ttggtatcaa caccgaccat gatgccgtta acatcctgat catagttgat
     7441 ggtgccgttg tcgccgttga agttaccacc gaagtagctt acccatgcgc cgccgttatc
     7501 agccaggcca tgacgagagt tggtcagacg agtaccaacg gtgtcttgtt ccaggttcca
     7561 gatattggtg ttcgcagacg ggatgctcag cgccatgtta gcgtagtcgg tcagctccat
     7621 ctgttgcaga acaacggtgt taccgcgctg ttcagcctga taggtgtatg cacccaggtc
     7681 agctttgtta gcagcagaga aggtcgcgtt gctgtttttg tcgttgacgt agatcagctc
     7741 gttgccgttg tagtcagcga tagaaccagc accagttgcg ttgtcgatac gaactttgta
     7801 gttaccggtt gcagctgcaa cgttatcaac agagctgtta tccagttcag taccgttgtc
     7861 tacgtcgccg ttaccattga tagtcaggtg accatcagag ttcatagcaa caataccgta
     7921 accgtagtta gacttggaag tatcccacgt acggtcgttc accagatcag cgttcagaac
     7981 gtaatcgctg ctgttgatgt cgaacacacc agaaattacg ttgccgttat ggtcagcaat
     8041 gttgccgctg gtcagttcca gtttatcagt acggaaagct tcagtatcga actgatcaac
     8101 attcacatcc agcacaccac cgttagtcac agtgatagta ttggtataca gattagaata
     8161 attattccaa ccggtagatt cggttaaatt aacggtactg tagctatcaa cagtcaggtg
     8221 gtctgtagca accacgccat ttgcaccaat attcagtgaa gaaccgttgg ttaaaccaat
     8281 ggtatctgat accagagagg aatcagtaac atttacctga gagccattat ttacagctaa
     8341 tgtatcaatt actgaactat taacggtatt ccattctgaa ctattgttca gtgctacgtt
     8401 aaatacgccg ctctggaata ctttgttgtc aataatacgg ccccaatcat tagcttcgat
     8461 ggtggcacca ggggtcaggc tgttcggcgc aacgtcatac atatctgccg gagcaattac
     8521 ttcagcactc atgtttgctg aaccaaccca cttactaccg ttattcagag taatattcag
     8581 ttcgtcaacg ttctgagcgt catcta
//
LOCUS       DAZKHC020000154         8381 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZKHC020000154 DAZKHC020000000
VERSION     DAZKHC020000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.154"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(88..978)
                     /gene="malG"
                     /locus_tag="ACHOSM_RS21845"
                     /old_locus_tag="pgaptmp_004369"
     CDS             complement(88..978)
                     /gene="malG"
                     /locus_tag="ACHOSM_RS21845"
                     /old_locus_tag="pgaptmp_004369"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HGU4560734.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(993..2537)
                     /gene="malF"
                     /locus_tag="ACHOSM_RS21850"
                     /old_locus_tag="pgaptmp_004370"
     CDS             complement(993..2537)
                     /gene="malF"
                     /locus_tag="ACHOSM_RS21850"
                     /old_locus_tag="pgaptmp_004370"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HGU4560735.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMILACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(2691..3881)
                     /gene="malE"
                     /locus_tag="ACHOSM_RS21855"
                     /old_locus_tag="pgaptmp_004371"
     CDS             complement(2691..3881)
                     /gene="malE"
                     /locus_tag="ACHOSM_RS21855"
                     /old_locus_tag="pgaptmp_004371"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HGU4560736.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4246..5361
                     /gene="malK"
                     /locus_tag="ACHOSM_RS21860"
                     /old_locus_tag="pgaptmp_004372"
     CDS             4246..5361
                     /gene="malK"
                     /locus_tag="ACHOSM_RS21860"
                     /old_locus_tag="pgaptmp_004372"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HGU4560737.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5433..6773
                     /gene="lamB"
                     /locus_tag="ACHOSM_RS21865"
                     /old_locus_tag="pgaptmp_004373"
     CDS             5433..6773
                     /gene="lamB"
                     /locus_tag="ACHOSM_RS21865"
                     /old_locus_tag="pgaptmp_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HGU4560738.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPAEFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            7016..7936
                     /gene="malM"
                     /locus_tag="ACHOSM_RS21870"
                     /old_locus_tag="pgaptmp_004374"
     CDS             7016..7936
                     /gene="malM"
                     /locus_tag="ACHOSM_RS21870"
                     /old_locus_tag="pgaptmp_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HGU4560739.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            8165..8381
                     /locus_tag="ACHOSM_RS21875"
                     /old_locus_tag="pgaptmp_004375"
                     /pseudo
     CDS             8165..8381
                     /locus_tag="ACHOSM_RS21875"
                     /old_locus_tag="pgaptmp_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2054 a         2176 c         2170 g         1981 t
ORIGIN      
        1 gcgctccgtg gagcgccgaa taacagtcac aagttgggat aacgtaagtt gagggtgcag
       61 cggcataaca ttggcagaac aacatcttta acctttcaca ccacctgccg tcaggccgtt
      121 gaccagccag cgttgagcca gcaagaagac gatggtgatc ggtaatgcag acatcacggc
      181 agcggcggca aagtcacccc acaggtagtt ttgcgggttg aggtattgct gcatccccac
      241 ggccagggtg tagctgttta cgtcacgcag taacagcgac gcgaccggaa cttcagtaat
      301 ggcagcgata aacgacagga tgaataccac cgccagaatc ggtactgaca gcggcaacag
      361 gacaaggcgg aaggcctgcc acggtgtcgc accatccagc gcagccgctt cttccagcga
      421 actgtcgatg gtttcgaaat agcctttgat ggtccagaca tgcagcgcaa tcccacccag
      481 atacgcgaaa attacgccgc cgtgagtatt caggccaatg aatggaatgt actcacccag
      541 acgatcaaac aacgcataca acgcgaccag tgaaagtact gccgggaaca tctggaaaat
      601 cagcattcct ttcagcagcg tcgctttgcc tggaaagcgc atacgggcga aagcgtaggc
      661 gcaggtggtg gagagcgcca caatgccaat cgcggaaatc ccggcgacct ttaccgagtt
      721 ccacagccac agcagtaccg ggaatggcgg tggcgtaatg cgaccatcag cctgttcaac
      781 gctaaaacct aacgccagtt tccagtgatc ccaggagatt tgctccggga tcaggctacc
      841 ggtcgcgaag tttccctgac gcagcgagat agcgacgacc atcagcagcg ggaacataat
      901 cgccgcgata aaaagtagca gtagcaggtg agtaataaac aaacgtgctt tttgcgattt
      961 cggttggacc attgccattt ttgttatctc ccttaatcaa acttcattcg cgtggctttc
     1021 aggttcacta tcgccagcgc acccaccagc aggaagatca gcgtggcaat tgctgccgcc
     1081 agaccgaagt cctgaccccc gccgccttca aaagcgatgc ggtaggtgta gttaacaagc
     1141 aggtcggtat aaccggctgg cgtggtcgtg ccaagacgat ccgggccgcc gttggttaac
     1201 agttgaatca gcacgaagtt gttaaagtta aaggcgaagc tggcgatcat cagcggcgtc
     1261 agcggtttaa tcagcagcgg cagcgtaatc ttaaagaagt tctggaacgg acctgcacca
     1321 tccattgctg aggcttcata caaatcgtcc ggaatcgctt tcagcaagcc catgcagagg
     1381 atcatcatgt acggataacc cagccaggta ttgacgataa tcagcatcgt gcgggcggtg
     1441 gtcggatcgc tgaaccaggc gggcttcacg ccaaacagcg cgctcaacat catgttgatt
     1501 tcaccgaagc tctggttaaa caatcctttg aaaatcaaga ttgaaatgaa cgatggcacc
     1561 gcgtagggca gaatcagcag gacgcgatag accgctttgc cgcgcaacgc ttcccactgc
     1621 accagacacg ccagaatcat gccgactgcc accgttaaaa agacagtgat tagcgagaat
     1681 accacggtcc agacgaaaat ggcgaggaac ggtttctgaa tgccttcgtc ggtgaagacg
     1741 cgggtaaagt ttttccagcc ggtggtcacg gtgtaaccgg ggcttagctt ttcatccccc
     1801 cagttgccgt cggcggtaat ggactggtaa aagccaattt ggttattcgg acgatatttc
     1861 acgccgctct gattattcgt caacgtgccg tcaccgtcga gtgtgtagag cggctgcgtg
     1921 ccagaaaact ggcgcaggga gctcatcatc actttgttgc catccggcag aatggcggta
     1981 atgtcactca gcgcctggcg gttctgggta atcacgcgca gattcgcgcg ttcgccttcg
     2041 ggctgggcgg tcgtttcttt cagttgcagt ttttgctcgc cgccaaattt aaacgcgtcg
     2101 gagaggtaat ttttgccggt ttcgccgtcg ctgagcgcca gttgccactc atcgcccgcc
     2161 gggtaaagac caaagttata ggttttgcct gcttgccagg agcgatctaa caacacttcc
     2221 tgcgcacgtt caaaagtcag ctggttagtg ctgctgtagt tggtgaaggc aatggcgatg
     2281 gtgcagacca gagggaagag gacgaataat cccattccag ccattcccgg gtaaacatag
     2341 cgccaggcgt aggctttacg attggcgaaa atatacagcc ccgctgaact caatatcagc
     2401 gtggtaatgg cgaacaggta ttccccttgt gcgtacatta aaacaacaag gtaacccacc
     2461 agcaggccga gcagacctag cactgaccat ttcagcgcgt cgctttgcca ccaatgtttc
     2521 tttttaatga catccatggg gttcttcctc attccaggac ggataaggct ttcacgcctt
     2581 atccgacaac aactgcctga tgcgacactg atgcgtctta tcaggcctac atgcgtttca
     2641 gttttgtagg ccggacaagg cgttcacgcc gcatccggca tttcatagca ttacttggtg
     2701 atacgagtct gcgcgtcttt cagggcttca tcgacagtct gacgaccgct ggcggcgttg
     2761 atcaccgcag tacgcacggc ataccagaaa gcggacatct gcgggatgtt cggcatgatt
     2821 tcacctttct gggcgttttc catggtggcg gcaatacgtg gatctttcgc caactcttcc
     2881 tcgtaagact tcagcgccac tgcacccagc ggtttgtctt tattgaccgc ttccagacct
     2941 tcgtcagtca gcagatagtt ttcgaggaac tctttcgcca gctctttgtt cggactggcg
     3001 gcgttaatac cggcgctcag cacgccaacg aacggtttag atggttgacc cttgaaggtc
     3061 ggcagtaccg ttacaccata attcactttg ctggtgtcga tgttggacca tgcccacggg
     3121 ccgttgatgg tcatcgctgt ttcgccttta ttaaaggcag cttctgcgat ggagtaatcg
     3181 gtgtctgcat tcatgtgttt gtttttaata aggtcaacca ggaaggtcag acccgctttc
     3241 gcgccagcgt tatccacgcc cacgtcttta atatcgtact tgccgttttc atacttgaac
     3301 gcataacccc cgtcagcagc aatcagcggc caggtgaagt acggttcttg caggttgaac
     3361 atcagcgcgc tcttaccttt cgctttcagt tctttatcca gcgccgggat ctcttcccag
     3421 gtttttggtg ggttcggcag cagatctttg ttataaatca gcgataacgc ttcaacagcg
     3481 atcgggtaag caatcagctt gccgttgtaa cgtacggcat cccaggtaaa cggatacagc
     3541 ttgtcctgga acgctttgtc cggggtgatt tcagccaaca ggccagattg agcgtagcca
     3601 ccaaagcggt cgtgtgccca gaagataatg tcagggccat cgccagttgc cgcaacctga
     3661 gggaatttct cttccagttt atccggatgc tcaacggtga ctttaattcc ggtatctttc
     3721 tcgaatttct taccgacttc agcgagaccg ttatagcctt tatcgccgtt aatccagatt
     3781 accagtttac cttcttcgat tttggcgaga gccgaggcgg aaaacatcat cgtcgttaat
     3841 gcggataatg cgaggatgcg tgcacctgtt tttattttca taatctatgg tccttgttgg
     3901 tgaagtgctc gtgaaaacac ctaaacggac tctagtttct ttatacggca acctctttcc
     3961 atcctccttg cccctacgcc ccaccgttgc tttgtgtgat ctctgttaca gaattggcgg
     4021 taatgtggag atgcgcacat aaaatcgcca cgatttttgc aagcaacatc acgaaattcc
     4081 ttacatgacc tcggtttagt tcacagaagc cgtgttctca tcctcccgcc tcctccccca
     4141 taaaaaagcc agggggtgga ggatttaagc catctcctga tgacgcatag tcagcccatc
     4201 atgaatgttg ctgtcgatga caggttgtta caaagggaga agggcatggc gagcgtacag
     4261 ctgcaaaatg taacgaaagc ctggggcgag gtcgtggtat cgaaagatat caatctcgat
     4321 atccatgaag gtgaattcgt ggtgtttgtc ggaccgtctg gctgcggtaa atcgacttta
     4381 ctgcgcatga ttgccgggct tgagacgatc accagcggcg acctgttcat cggtgagaaa
     4441 cggatgaatg acactccgcc agcagaacgc ggcgttggta tggtgtttca gtcttacgcg
     4501 ctctatcccc acctgtcagt agcagaaaat atgtcatttg gcctgaaact ggcaggcgca
     4561 aaaaaagagg tgattaacca acgcgttaac caggtggcgg aagtgctgca actggcgcat
     4621 ttgctggatc gcaaaccgaa agcgctctct ggcggtcagc gtcagcgtgt ggcgattggc
     4681 cgtacgctgg tggccgagcc aagcgtattt ttgctcgatg aaccgctctc caacctcgat
     4741 gctgcactgc gtgtgcaaat gcgtatcgaa atctcccgtc tgcataaacg cctgggccgc
     4801 acaatgattt acgtcaccca cgatcaggtc gaagcgatga cgctggccga caaaatcgtg
     4861 gtgctggacg ccggtcgcgt ggcgcaggtt gggaaaccgc tggagctgta ccactatccg
     4921 gcagaccgtt ttgtcgccgg atttatcggt tcgccaaaga tgaacttcct gccggtaaaa
     4981 gtgaccgcca ccgcaatcga tcaagtgcag gtggagctgc cgatgccaaa tcgtcagcaa
     5041 gtctggctgc cagttgaaag ccgtgatgtc caggttggag ccaatatgtc gctgggtatt
     5101 cgcccggaac atctactgcc gagtgatatc gctgacgtca tccttgaggg tgaagttcag
     5161 gtcgtcgagc aactcggcaa cgaaacccaa atccatatcc agatcccttc cattcgtcaa
     5221 aacctggtgt accgccagaa cgacgtggtg ttggtagaag aaggtgccac attcgctatc
     5281 ggcctgccgc cagagcgttg ccatctgttc cgtgaggatg gcactgcatg tcgtcgactg
     5341 cataaggagc cgggcgttta agcaccccac aaaacacaca aagcctgtca caggtgatgt
     5401 gaaaaaagaa aagcaatgac tcaggagata gaatgatgat tactctgcgc aaacttcctc
     5461 tggcggttgc cgtcgcagcg ggcgtaatgt ctgctcaggc aatggctgtt gatttccacg
     5521 gctatgcacg ttccggtatt ggctggacag gtagcggcgg tgaacaacag tgtttccaga
     5581 ctaccggtgc tcaaagtaaa taccgtcttg gcaacgaatg tgaaacttat gctgaattaa
     5641 aattgggtca ggaagtgtgg aaagagggcg ataagagctt ctatttcgac actaacgtgg
     5701 cctattccgt cgcgcaacag aatgactggg aagctaccga tccggccttc cgtgaagcaa
     5761 acgtgcaggg taaaaacctg atcgaatggc tgccaggttc caccatctgg gcaggtaagc
     5821 gcttctacca acgtcatgac gttcatatga tcgacttcta ctactgggat atttctggtc
     5881 ctggtgccgg tctggaaaac atcgatgttg gcttcggtaa actctctctg gcagcaaccc
     5941 gctcctctga agctggtggt tcttcctctt ttgccagcaa caatatttat gactatacca
     6001 acgaaaccgc gaacgacgtt ttcgatgtgc gtttagcgca gatggaaatc aacccgggcg
     6061 gcacattaga actgggtgtc gactacggtc gtgccaacct gcgtgataac tatcgtctgg
     6121 ttgatggcgc atcgaaagac ggctggttat tcactgctga acatactcag agtgtcctga
     6181 agggctttaa caagtttgtt gttcagtacg ctactgactc gatgacctcg cagggtaaag
     6241 gtctgtcgca gggttctggc gtcgcgtttg ataacgaaaa atttgcctac aatatcaaca
     6301 acaacggtca catgctgcgt atcctcgacc acggtgcgat ctccatgggc gacaactggg
     6361 acatgatgta cgtgggtatg taccaggata tcaactggga taacgacaac ggcaccaagt
     6421 ggtggaccgt tggtattcgc ccgatgtaca agtggacgcc aatcatgagc accgtgatgg
     6481 aaatcggcta cgacaacgtc gaatcccagc gcaccggcga caagaacaat cagtacaaaa
     6541 ttaccctcgc acaacaatgg caggctggcg acagcatctg gtcacgcccg gctattcgtg
     6601 tcttcgcaac ctacgccaag tgggatgaga aatggggtta tgactacaac ggcgatagca
     6661 aggttaaccc gaactacggc aaagccgttc ctgctgagtt caacggcggc agcttcggtc
     6721 gtggcgacag cgacgagtgg accttcggtg cccagatgga aatctggtgg taatagcaaa
     6781 acctgggccg gataaggcgt ttacgccgca ttcggcaacc aacgcctgat gcgacgcttg
     6841 cgcgtcttat caggcctaca acggctgtca aatgtaggcc ggataaggcg tttacgccgc
     6901 atccggcata aaaacaggtt gtcattatct gaaaggggcg aaagcccctc tgattatcgg
     6961 gtttagcgcg ctattgcctg gctaccgctg agctccagat tttgaggtga aaacaatgaa
     7021 aatgaataaa agtctcatcg ccctctgttt atcagcaggg ttactggcaa gcgcgcctgg
     7081 aatcagcctt gccgatgtta actacgtacc gcaaaacacc agcgacgcgc cagccattcc
     7141 atctgctgcg ctgcaacaac tcacctggac accggtcgat caatctaaaa cccagaccac
     7201 tcaactggcg accggcggcc aacaactgaa cgttcccggc atcagtggtc cggttgctgc
     7261 gtacagcgtc ccggcaaaca ttggcgaact gaccctgacg ctgaccagcg aagtgaacaa
     7321 acaaaccagc gtttttgcgc cgaacgtgct gattcttgat cagaacatga ccccatcagc
     7381 cttcttcccc agcagttatt tcacctacca ggaaccaggc gtgatgagtg cagatcggct
     7441 ggaaggcgtt atgcgcctga caccggcgtt ggggcagcaa aaactttatg ttctggtctt
     7501 taccacggaa aaagatctcc agcagacgac ccaactgctc gacccggcta aagcctatgc
     7561 caagggcgtc ggtaactcga tcccggatat ccccgatccg gttgctcgtc ataccaccga
     7621 tggcttactg aaactgaaag tgaaaacgaa ctccagctcc agcgtgttgg taggaccttt
     7681 atttggttct tccgctccag ctccggttac ggtaggtaac acggcggcac cagctgtggc
     7741 tgcacccgct ccggcaccgg tgaagaaaag cgagccgatg ctcaacgaca cggaaagtta
     7801 ttttaatacc gcgatcaaaa acgctgtcgc gaaaggtgat gttgataagg cgttaaaact
     7861 gcttgatgaa gctgaacgcc tgggatcgac atctgcccgt tccaccttta tcagcagtgt
     7921 aaaaggcaag gggtaattac gccccacagt gctgattttg caacaactgg tgcgtctcct
     7981 ggcgcacctt tttttatgct tccttcctgg gatatgagcg attttttata gtaactcact
     8041 tcttcttcac taagaatatc cattatctca atgccttatc agagattctt ttcctttcgc
     8101 cggtagtgtc tggacattca ggctactttt ccaggttatt ttatttctgt tatgcagagg
     8161 ttttatgata agtcatatcc taaattctgg cggcaataac tctttgatga aacatgatgt
     8221 ggtgcaagga aataatatag tagatcttga tttactacgt aatttcaatg gggtgccagg
     8281 tttaaacaga gataacttta tttatatcag caatattttt tcaaatataa agcaacggaa
     8341 cgaaaaaatc atgcaataaa tatgtttcgt gaagtctcaa t
//
LOCUS       DAZKHC020000155         8329 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZKHC020000155 DAZKHC020000000
VERSION     DAZKHC020000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.155"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..657)
                     /gene="kdpE"
                     /locus_tag="ACHOSM_RS21880"
                     /old_locus_tag="pgaptmp_004376"
     CDS             complement(<1..657)
                     /gene="kdpE"
                     /locus_tag="ACHOSM_RS21880"
                     /old_locus_tag="pgaptmp_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HGU4560740.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLLQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATTAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     I"
     gene            complement(654..3338)
                     /gene="kdpD"
                     /locus_tag="ACHOSM_RS21885"
                     /old_locus_tag="pgaptmp_004377"
     CDS             complement(654..3338)
                     /gene="kdpD"
                     /locus_tag="ACHOSM_RS21885"
                     /old_locus_tag="pgaptmp_004377"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HGU4560741.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHLEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            complement(3331..3903)
                     /gene="kdpC"
                     /locus_tag="ACHOSM_RS21890"
                     /old_locus_tag="pgaptmp_004378"
     CDS             complement(3331..3903)
                     /gene="kdpC"
                     /locus_tag="ACHOSM_RS21890"
                     /old_locus_tag="pgaptmp_004378"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HGU4560742.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYRQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            complement(3912..5960)
                     /gene="kdpB"
                     /locus_tag="ACHOSM_RS21895"
                     /old_locus_tag="pgaptmp_004379"
     CDS             complement(3912..5960)
                     /gene="kdpB"
                     /locus_tag="ACHOSM_RS21895"
                     /old_locus_tag="pgaptmp_004379"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HGU4560743.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAVDKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            complement(5983..7656)
                     /gene="kdpA"
                     /locus_tag="ACHOSM_RS21900"
                     /old_locus_tag="pgaptmp_004380"
     CDS             complement(5983..7656)
                     /gene="kdpA"
                     /locus_tag="ACHOSM_RS21900"
                     /old_locus_tag="pgaptmp_004380"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HGU4560744.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMVGLTVQNFLSAASGIAMIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICTGVVMWAEVQGNPHLLALGADNSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPTSSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            complement(7656..7745)
                     /gene="kdpF"
                     /locus_tag="ACHOSM_RS21905"
                     /old_locus_tag="pgaptmp_004381"
     CDS             complement(7656..7745)
                     /gene="kdpF"
                     /locus_tag="ACHOSM_RS21905"
                     /old_locus_tag="pgaptmp_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HGU4560745.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            8058..8264
                     /locus_tag="ACHOSM_RS21910"
                     /old_locus_tag="pgaptmp_004382"
     CDS             8058..8264
                     /locus_tag="ACHOSM_RS21910"
                     /old_locus_tag="pgaptmp_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HGU4560746.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMEQAEKATGDFY"
BASE COUNT         1996 a         2428 c         2177 g         1728 t
ORIGIN      
        1 aataccggtt tcagtaatga aatggcgggg gcgagcggga tcctgttcca gtttttgtcg
       61 cagatgtccc atataaatac gcaaatagtg actgtgttcg accgcgtttg gcccccacac
      121 ttggttaagg agctggcgct gggtgagtac ttttccggca ttgttgagca gcaccgccag
      181 caggcggaat tcaatcgggg tgagatgcac ctcttcctca ccccggtgaa tcacgcgggc
      241 ggctaaatcg acggtaacat cggaaaattt caccagcgga tcgggcgcgg tggtggcaga
      301 gtggcggcgt aatgcgacgc gcagacgggc ctgtaattcg ccaatgccaa acggcttact
      361 cagataatca tccgctccgg catccagcgc ggcgattttg tcgctctctt cgctgcgtgc
      421 ggaaagcaca atcaccggca ccgcgctcca ctggagcagg tcgcggataa actcaatccc
      481 atcaccatcg ggcaggccga gatcgagaat aatcaaatct ggcttacggg ttgccgcttc
      541 cagcaagccg cgttgcagcg tttcggcctc aaagacgcgc ataccgtcgc cctccagcgc
      601 cgtacgcaga aagcgacgaa tagcctgttc atcttcaaca atcagaacgt ttgtcacata
      661 tcctcatgaa agtcttcaag ttcaggagcg gtttgctggg gaagtgtaac acgaaaacag
      721 gcaccacctt ccggtcggtt gaacgcggta atggtgccgc cgtgtacatc cacaatcgcc
      781 cgacaaattg ccaacccaag cccgacaccc ggcaccgccg actctttatt cccgcgagca
      841 aacttatcaa atatcgtctg ttcctggcct ggcggaagac cggggccgtt atcccagaca
      901 tccagttgta gattttcgcc ctcaaggtgg gcatcgatac caatttcggc ctgcgcaccc
      961 gcatatttca ccgcgttctc cagcagatta atcagcaccc gttcaaagag tggcccgtca
     1021 acgtggatta aggtcagcgg ttctggcaga gaaagattga tgggcgacga taaacccggt
     1081 tccagcattt gcagcgcgct gccgactact tcttccagcg ttaaccactc tttcttcaaa
     1141 ttaaagccgc cggactgaat tcgcgccata tccagtagat tattcaccag tcgggtagtg
     1201 ttcagcacat gctgacgaat ctcgctggcc tggcgggcgt ggggcgagcc ttcgcttgcc
     1261 agatcgagcg ttaagatttc tgcctgaccg aacagcaccg tgagcggcgt gcgtaaatca
     1321 tgcgaaagcg ccgccagcag agcgttgcgg atctgttcac gttcgcttgc catccgcgcc
     1381 tgttcttcgc tggcggtcag tgtcagccgc tcaagggcat tggcgactaa caacgtaaat
     1441 gtctccagca ggcgctgttg ttccgggatc atcaactggc gcagattccc cggctccacc
     1501 accaccagcc cgtaggtttt ctcaccgctt tttaacggca aaatttggta cggcacgccg
     1561 ggtaatgtgt cggtgcccgc gcccgcaggc aggcctttat caaaactcca ctgcgcgatg
     1621 gcatcgtccc acggcgtcat tccttgcgga tgtgttaacg gctgcaattt accgttgtca
     1681 tcgggcaaca acacctgact gcgggcatga aacgtggagg caataaattg ttcgctggtg
     1741 gcggcgatgt cctgcggact gcggcccacc gccagagctt tcgacatttc atataagtgc
     1801 cgtgtgcgtt gctcgcggta acgggctacc cgcgcctgat aacgcacacc cgcagtaagg
     1861 ttcccgatca ccagcccgac ggttaacatc accgcgaagg tcagcagata ttgcacatca
     1921 gagacggcga gcgtgccgcg tggggcgata aaaaagagat cgaaactcac cacattaatg
     1981 acggtagcaa ccacagaagg ccagcgtcca taaaatagcg ccaccaccac cacgccaagt
     2041 agatacagca tcaccaggtt ggcggcatca aacgccatca gccactgcat ggcaattaag
     2101 gtgataacgg cgcataacgc ggcggcaacc acgcatccct gaatctgtac ccgccacttg
     2161 tctttaaaag agcggctatc cggcgcgttg ttaatcgtgc gggcgggtgg ttcatcaagc
     2221 gcgaccagca cctgatcgag atcgggggcg atgcgcgcca gtcggtcagc aaacgtttcc
     2281 cgacgccacc agcggcgcga ggctgggcga ccgagaataa tcttgccgag attatgttca
     2341 cgggcataac gcactaccgc tttctcttcc gctggatcag aaagtgttgc cgtctccgcg
     2401 cccagctcct gcgccagacg taaggcgctg agaattgccc gacgtttttt ctccggtaag
     2461 cgatgcaggg caggggtttc aacatacacc gcgtgccaga cgctacccag ccgcgacgcc
     2521 agccgcgccg ccgctcggac cagtttctca ctgccggtgt tatgcccgat gcataaaagg
     2581 atcgcgtcgc gcgtgtgcca cactttctct tcgccaggat gcccccgcca ggcgcgcatt
     2641 tgctcatcta cgcgatcggc agtacggcgc agtgccagtt cgcgcagggc gatcagattg
     2701 cctttgcgga aaaaatgttc aatggcgcgc tccgcctgcc cggcaatata gactttgcct
     2761 tctttcagcc gctggcgcag atcgtccggg ggcaagtcca ccagaaccac gtcgtcggcg
     2821 gcatcgaaaa aaggatcggg cacggtttcc cgtacctgaa ttccggtgac gccgctgacc
     2881 acatcattca ggctttccag atgttgaacg ttgacggtag taaaaacatc aatgcctgct
     2941 tccagcagct cttcaatatc ctgccagcgt ttgggatgac gagaacctgg cgcattactg
     3001 tgcgccagtt cgtccattaa gatcagcgcc ggacggcggg cgagggcggc atcgaggtca
     3061 aactcgcaga tgtgccgccc acggtacgcc aggcgttttg gcggcagaac agccagccct
     3121 tccagcattg cggcggtatc ttttcgcccg tgggtttcta ccacgccaac cacaatatcc
     3181 agcccttgcg cccgtagtcg ctgggcttct gccagcatcg cccaggtctt cccgacgcct
     3241 gcacaggcac cgaagaaaac tttcagcttc ccccgatgcg gcgcggcagt ttgttccagc
     3301 agacgatcgg ggtcgggacg taagggttcg ttattcatca agcttatcca gcgccagatt
     3361 gagttcgaca atgttgacga ccggctggcc gatatatttc accagcggtt gttgcctgta
     3421 ttttgcgatc agttgcgtga gctgttcaac gctgagatta cgcgctttcg ccacgcgtgg
     3481 gatttgccag accgccgctt gcggggtgat attattgtcc agcccgcttg ccgatgccgt
     3541 caccagttca accggaacgt tcgtactggc atccgggtta gcggcccgta atgcagcaac
     3601 gcgtgcggct atttgtttat ccagctcagg gttactgacc gccagattgc tcccgccaga
     3661 agcctgtgga ttatagggca tttctgccgt tgccgacggg cgaccctgaa aatagccgtt
     3721 gccggtaaaa ttctgcccga ttaatgccga accccgcacc gtatcacctt cacgaatcaa
     3781 cgaaccgttg gcctgccagg gaaaccacca ttgccccagc acggtggtca gcagcgggta
     3841 aacgccgcca gtaatcaata acagaaagat aaatgtcgat aatgccggac gtaagccacg
     3901 catggtaaac ctcacaccag accgcaaacg gtcagcagta aatcaatgac tttgatacca
     3961 ataaacggca ccagcagccc acccagaccg taaatccata agttacggcg caacatggca
     4021 gaagcggtaa gcggtttata actcacgcct tttaacgcca gaggaatcaa aaagacgata
     4081 atcaaggcgt tgaaaatgac cgcactgaga atggcggagt cgggcgaatg aaggcgcatg
     4141 atgttcagcg catttaactg cggatacgtt gccgcgaacg ccgccggaat aatggcgaag
     4201 tatttcgcca catcgttggc aatgctgaag gtggtcagcg agccacgggt catcagcatc
     4261 tgtttgccaa tatgcaccac ctcgatcaac ttggtcgggt tagagtcgag atcgaccata
     4321 ttgcccgcct ctttcgccgc ctgggtgccg gagttcatcg ccaccgcgac atctgcctgc
     4381 gccagcgccg gagcatcgtt ggtgccgtcg ccggtcatcg ccaccaaacg gccttccgcc
     4441 tgatactgac gaatcaatgc cagcttggcc tccggtgtcg cttcggcgag aaaatcatcg
     4501 acgcccgctt ccgcagcaat ggcggcggca gtcagacggt tatcgccggt aatcatcacc
     4561 gttttaatgc ccattttgcg cagctgggcg aagcgctctt taataccgcc tttgacgata
     4621 tctttcagcg caataacgcc cagcacacgg gaaccttcca ccaccaccag cggcgtggcc
     4681 ccctgacgcg caacctgatc gactttttga tcaacatcgg cagggaagtg accgccgtta
     4741 gcctcaacat ggcggcgaat ggcatcgaca gaacctttac ggatcatgcg gttgtcgatg
     4801 ttgatcccgc tcatccggct ttgcgcagta aacggtacaa aggtggcatg gagcgactgc
     4861 acatcgcgct cgcgcaggtt aaaacgttgc ttggcgagga tcacaatact gcggccttcc
     4921 ggcgtttcat cagccagcga agccagttgt gcggcgtcag ccagcgtttt ttcatccacg
     4981 ccctgcgcag ggataaactc cgatgcctga cggttaccga gtgtgatggt gccggtttta
     5041 tccagcagca gaacgtcaac gtcacctgcc gcttcaactg cacgtccgct ggtggcaatc
     5101 acattcgcgc ccagcatccg actcatcccg gcgacgccga tcgctgacaa caggccgcca
     5161 atagtggttg ggatcagaca gaccagtagc gccaccagta ccgttacgct gaccgcgttg
     5221 ccgccccacg cggaaaacgg ccacagcgtg gcggttgcca gtaaaaagac gatggtcaga
     5281 gcaatcagca ggatagtcag ggcaatctcg ttcggtgttt tgcgtcgctg tgcgccttcc
     5341 accatcgcga tcatccgatc cagaaatgtc tcaccggggt taacgctaca ctcaatcacc
     5401 agccagtcag aaagaatacg cgtgccgccg gtgacggagg caaaatcgcc gccggattca
     5461 cggatcaccg gtgccgattc cccggtgatg gcgctttcat cgaccgatgc gccgccttca
     5521 atgacttcac catcgcaggg gataatatcg ccagcttcta ccagcacgat gtcaccttta
     5581 cgcagttgat cggcaggaac tttgtccacc gcagcgccat atttcggctc acgcagcttg
     5641 cgggcaaagg cagttttttt caccccttta agactgttgg cctgcgcttt actgcggcct
     5701 tctgccagcg cctcggcgaa attagcgaac agtacggtga cccacagcca gccgctaatt
     5761 gccgcgctaa acagcgcatt gccaggcatc gcaccgcttg ccatcgcgat gctaatacag
     5821 gtggtcagca gactgccgat ccagacgata aacatcaccg gattgcgcca ttgcgcctgc
     5881 gggtttaatt ttttcaccgc ttctttcaga gcctgaacga caagtgttgg ttcgaatagc
     5941 gccagttgtt tacgactcat attcagtgct cactcaatat catcaggaga gatattccgc
     6001 caccggacca agcgccaggg cagggataaa ggtcagtgcg ccaaccagca acacggtgcc
     6061 gattaacagg ccaacaaaca gcgggccgtg cgttggcagc gtgccggagc tggtgggttg
     6121 gctcttttta ctcaccagcg aaccggcaat tgccatcacc gggataatca ccccgaagcg
     6181 accgacaaac atgcagaacg ccagtaaaca gttccagaac ggagagttgg cgcttaatcc
     6241 ggcaaaggcg ctgccgttgt tgttagcggc ggacgacacg gcgtacagca cttcgctaaa
     6301 accatgcggg ccagggttaa gcatggcgct acgtccggcg tcggtcatca tcgccaacgc
     6361 cgcgcccatc agtaccagtg ttggagtaac cagaatcgcc agtgcagtca gtttcatctc
     6421 gcgtacgtcg atttttttac ccagatattc cggtgtacga ccaatcatca gcccggcaat
     6481 aaacaccgcc agcaggacaa acagcatcat gccgtaaaga ccagaaccga caccgccgaa
     6541 caccacttca ccaatttgca tcagccacat cggcaccatg ccaccgagag cggtaaacga
     6601 atcatgcatc gcaatcaccg cgccacagga agccgccgta gtcacgaccg caaacaggct
     6661 actgaccagc acgccgaaac ggctctcttt accttccata ttgatgctgt tgtccgcacc
     6721 cagtgccagc agatgaggat taccctgaac ttctgcccac atcaccacgc ctgtgcagat
     6781 gacaaaaatt actgacatcg cccacagcaa catgcgcccc tggcgacgat cgcccgccac
     6841 ttcaccaaag gcaaagcaca gcgctgttgg gatcaagaag atcgccagca tttgcacgaa
     6901 gttggtcagt gcggttgggt tttcaaacgg atgcgacgag ttggcattaa agaatccacc
     6961 gccgttagtg ccgagcatct tgatcgcttc ctgagaagcc accggaccca tgggtaacag
     7021 ctgttgcgct ccttcaacgg tattcactgc ctgatagggc agaaagtttt gcagtgcacc
     7081 ttgttgaata aaaaacagtg caatcaacaa cgccacaggg actagcaccc ataacgtgat
     7141 gcgtagcaga tcgacccagg cattcccgag cgtgctcatg ctctggcggg taaacgcacg
     7201 gatgagggca aaaatcatcg caatcccgct ggcggcagaa agaaagtttt gcaccgttaa
     7261 gcccaccatc tggctgaaat agctcaacgt ggtttcaccg ctataagatt gccagttggt
     7321 attggtgaca aagctgacgg cagtattcag cgccagatcc catgacagcc ccggcaactg
     7381 ctgtggatta agcggcagat agtgctgacc gagcaacata aaaaaaagca ccgccagccc
     7441 cagcatgttc aggccgagaa tggcacagag atattgcttc cagttcatct catggtcaga
     7501 gacgccaggt aaacgaaaaa gtacgcgctc aacgcccgtc gtaccgggaa gaggaatatc
     7561 attaatcagc cgcgccagcc cgctacctaa gggacgcgcc agcaccatta ataccagtaa
     7621 aaacgtggca atcagtaaga acccttgcgc agccatcaga atgcctccgc attgatcagg
     7681 gcataaacca aataacccag taataaaaac accagcaaca cgccggctat cacgcctgca
     7741 ctcacagtgc acctccagtg gcctaaaagt gataccggaa gagtagaatt ctggctgcaa
     7801 agatttcgca aaaatctgcg gacggggtgt aaaaaaagta taaaaatggc aaaagccatg
     7861 atttaactaa tgtttagtat tgatttaact tttgtgtaac ttaattacag ggtgaatgta
     7921 aataaaccat caataagcaa aattaagtgg tcggatgagt agtaaaatta cacaaaaggc
     7981 ggtactattt tcatcagata aacaaattca tttttccggt gccattcacc gggtagatac
     8041 aaaaggggga gaaaagtatg gaactctaca gagaatatcc tgcatggctt atctttttac
     8101 gccgtactta tgcggttgca gcgggcgttc tggcgctgcc tttcatgctt ttctggaaag
     8161 accgcgcccg cttttacagc tacctgcatc gcgtctggtc gaaaaccagc gataaaccgg
     8221 tgtggatgga acaggccgaa aaggcaaccg gcgattttta ctgattgctg agttctgaat
     8281 acattgaaaa ccgccagcgg aaacgctggc ggttttttta ttaggaaga
//
LOCUS       DAZKHC020000156         8171 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZKHC020000156 DAZKHC020000000
VERSION     DAZKHC020000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.156"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..549)
                     /locus_tag="ACHOSM_RS21915"
                     /old_locus_tag="pgaptmp_004383"
     CDS             complement(<1..549)
                     /locus_tag="ACHOSM_RS21915"
                     /old_locus_tag="pgaptmp_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4560747.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAVWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHDKKN"
     gene            628..1083
                     /locus_tag="ACHOSM_RS21920"
                     /old_locus_tag="pgaptmp_004384"
     CDS             628..1083
                     /locus_tag="ACHOSM_RS21920"
                     /old_locus_tag="pgaptmp_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560748.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            complement(1290..1715)
                     /locus_tag="ACHOSM_RS21925"
                     /old_locus_tag="pgaptmp_004385"
     CDS             complement(1290..1715)
                     /locus_tag="ACHOSM_RS21925"
                     /old_locus_tag="pgaptmp_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4560749.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(1699..1920)
                     /locus_tag="ACHOSM_RS21930"
                     /old_locus_tag="pgaptmp_004386"
     CDS             complement(1699..1920)
                     /locus_tag="ACHOSM_RS21930"
                     /old_locus_tag="pgaptmp_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4560750.1"
                     /translation="MGRYMGVAQQTVWQWFSFGVPPKQVIPLCQLMKWEVTPHEIRPD
                     IYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            2080..2481
                     /locus_tag="ACHOSM_RS21935"
                     /old_locus_tag="pgaptmp_004387"
     CDS             2080..2481
                     /locus_tag="ACHOSM_RS21935"
                     /old_locus_tag="pgaptmp_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4560751.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            2509..2700
                     /locus_tag="ACHOSM_RS21940"
                     /old_locus_tag="pgaptmp_004388"
     CDS             2509..2700
                     /locus_tag="ACHOSM_RS21940"
                     /old_locus_tag="pgaptmp_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HGU4560752.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            2700..2987
                     /locus_tag="ACHOSM_RS21945"
                     /old_locus_tag="pgaptmp_004389"
     CDS             2700..2987
                     /locus_tag="ACHOSM_RS21945"
                     /old_locus_tag="pgaptmp_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4560753.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            3264..3419
                     /locus_tag="ACHOSM_RS21950"
                     /old_locus_tag="pgaptmp_004390"
     CDS             3264..3419
                     /locus_tag="ACHOSM_RS21950"
                     /old_locus_tag="pgaptmp_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4560754.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            3421..3549
                     /gene="ydfB"
                     /locus_tag="ACHOSM_RS21955"
                     /old_locus_tag="pgaptmp_004391"
     CDS             3421..3549
                     /gene="ydfB"
                     /locus_tag="ACHOSM_RS21955"
                     /old_locus_tag="pgaptmp_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HGU4560755.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            3561..3950
                     /locus_tag="ACHOSM_RS21960"
                     /old_locus_tag="pgaptmp_004392"
     CDS             3561..3950
                     /locus_tag="ACHOSM_RS21960"
                     /old_locus_tag="pgaptmp_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560756.1"
                     /translation="MEFKDLPKEIQEIAAHALHQRLNEVELESATKKYIDNMARNVRD
                     AFTGLYSVSVTNNQNTEETAKRIASVMGFHVEEKYSKKEFWKTTKKLQSENCHLLRQS
                     LLSMRKVIQMTQDYRNCSHYLKNSELS"
     gene            complement(4137..4322)
                     /locus_tag="ACHOSM_RS21965"
                     /old_locus_tag="pgaptmp_004393"
     CDS             complement(4137..4322)
                     /locus_tag="ACHOSM_RS21965"
                     /old_locus_tag="pgaptmp_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560757.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLDSIITEP
                     NQYQSDLLNKELNAARD"
     gene            4896..5084
                     /gene="dicB"
                     /locus_tag="ACHOSM_RS21970"
                     /old_locus_tag="pgaptmp_004394"
     CDS             4896..5084
                     /gene="dicB"
                     /locus_tag="ACHOSM_RS21970"
                     /old_locus_tag="pgaptmp_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4560758.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            5081..5272
                     /locus_tag="ACHOSM_RS21975"
                     /old_locus_tag="pgaptmp_004395"
     CDS             5081..5272
                     /locus_tag="ACHOSM_RS21975"
                     /old_locus_tag="pgaptmp_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4560759.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            5366..7801
                     /locus_tag="ACHOSM_RS21980"
                     /old_locus_tag="pgaptmp_004396"
     CDS             5366..7801
                     /locus_tag="ACHOSM_RS21980"
                     /old_locus_tag="pgaptmp_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4560760.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFTWQF
                     LEQYPAAQDCAYKFLVCEDKPGMPRPAIDSWDTEYMQENRWDEESASFIPVEPESDPI
                     NVNFDKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEI
                     NAMYPERKLFAIGWVKHKCKPGTKWPEIQAELRNWKKRQDAERKETGKYTSVVDLARA
                     KANRQHTENPAEKIPPVTAAIHREYKQTWKTLDRELAYYLWPSDADAGNIDGSILRWA
                     KNEVIARDREDWKRISASMRKQPDALRYSRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPDNEKNECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYLARKNGTYTDLPQNVDTAEDSVQ
                     PEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIETCDSV
                     NQNQSETEQNDQKAEQNSPNALQDEPETKQSEPVAQQEEEKVCTACGQSGGGNCPDCG
                     AVMGDATCQETFDEENQAEAQEEMEGSGGDHYHTTDNESGETANPLIKVNGHREITST
                     SRLWHHLMIDLETMGKNPDAPIISIGAIFFDPQTGEMGPEFSKTIDLETAGGVIDRDT
                     IKWWLKQSREAQSAILTDEIPLDDALLQLREFIDENSGEFFVQVWGNGATFDNVILRR
                     SYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGERHNALNDARYQAKYVS
                     AIWQKLLPSQADF"
     gene            7869..8111
                     /locus_tag="ACHOSM_RS21985"
                     /old_locus_tag="pgaptmp_004397"
     CDS             7869..8111
                     /locus_tag="ACHOSM_RS21985"
                     /old_locus_tag="pgaptmp_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGU4560761.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            8089..>8171
                     /locus_tag="ACHOSM_RS21990"
                     /old_locus_tag="pgaptmp_004398"
     CDS             8089..>8171
                     /locus_tag="ACHOSM_RS21990"
                     /old_locus_tag="pgaptmp_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560762.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKP"
BASE COUNT         2373 a         1958 c         1843 g         1997 t
ORIGIN      
        1 gttttttttg tcatgaacca tagtgttttt gtcctgttcc tgttctttct cctgctcctg
       61 ttcctgttct tggcttcgaa gccccttaaa agccccttcg aagcccctta ccgaatttcg
      121 gctattattc cgcctcacat ccagatggaa atccgtttta tatctgtcgt aaaacactga
      181 cagaaaagcg ttttcaggta atgatgcata ctcattcctg acacctgcac aacggttatc
      241 gccaggcttc aacgtttccc caacctgcca gactgccatt tcatggaccc agaccatctc
      301 tgcatcatgg tcatagctac aaaaaccagc ttcaacagcc cttttaagcc cctttgaagc
      361 cccttccaga ccaagcccgg tttcatgagc aaggtataaa actggcaggt aatacaaacc
      421 cagcatattt gcgtgagggg atgtcatcag gtaaaaagca acaacttgcg cttctgcacc
      481 cgcctttcta agttctctcc ccgtttctcc cagccagaat cgcggagaaa cttttgcgta
      541 atcacgcatg gctacctcat ctggtgccga accttcctcc ggatataatc tgtggttccc
      601 aatcgacaga accagaggag gttcgacatg tattttttga aaagccttta tcaggctcat
      661 gtattaaatg ttgcagcaac aaaccgctgg tgtaacagcc ccgaaatgct cccggattac
      721 agagcctggc tgcgcgccga aacatacctt cgtctcgaca tattgattag cgaacttcaa
      781 aaagagactg catccattca taaccttcag ggtatcgacg ctgttcgcat tctggtatcg
      841 cgccatagtg ctctctcaat aattgaagtg cgtcatctct ctttttctga actgattttc
      901 ttgcttcaac cagctctgga atcagcgaat atcccaccgg aagtgatcca atacccacct
      961 catgttgacg agcagttaca agatgtgcca tacaaccagc gtgctggatt gactccctgc
     1021 tcagaggctg aatgggatca ctctctgctg aagaaatacc aagatttgta taatcctcaa
     1081 taagcccggc acacgcttct gcatcagcca gctttattct ggtttccttg cgctgttctc
     1141 tggctgacaa acgccagagc agcgcattag ctttatgtat caaccactct gccagctcca
     1201 catcagataa accgccacgc cagatgtgcg gactgttttc gtaaacgctc agtgtcaatt
     1261 ttttgtcatt actcatattt attacccaat taatgcacag ccagagtgtt tcctgccggg
     1321 ccaccacgat tcatctgatc gaacagaacg atcgctgatg caacgaaatc atcaatatct
     1381 ttcaccagcc gttccttcgt ctctaccaac tcccgaaaat aagcggaact gtggctgcgc
     1441 attcgggcca ccagcggagg cggcatcgct ttttcgatcg ctggtaacaa cgcctgaatt
     1501 tttttaaccg catcaggagt gtctttctcc acccagcgga aaattttctg agtattgcga
     1561 gccatggctt ccggatggct gtcgtcatac agttccggga acgtcattcc cagctcgaaa
     1621 tacgctttgg taattttcgc agccggtact ttttcgccgt ccggatgcgc ccaggcattc
     1681 atcgccatgc ggatgtgttc atgcttgatt ttcatgaatc aactccggcg catttgatgt
     1741 gttaacctta catccaacag gtaaaccgtc ggttgggtta ggataaatat ctgggcggat
     1801 ttcatgcggg gtaacttccc acttcattag ttggcataac ggaattacct gctttggggg
     1861 aacgccaaag ctaaaccatt gccaaacagt ctgttgagcg acccccatat aacgacctat
     1921 ttcagcctga gtgtatttct acctaatttt ttcgcgagtg ctatctagca ttttgccctc
     1981 ctctaaaaac tataagcaaa gactacaata aaaaactgta tgcaatcaac agttttttat
     2041 tgtgatgctt ttaacagtat ttacctgtaa aattgaataa tgatgagcgc cctagaagta
     2101 tctatgtaca gaatcagcaa gcttcttcag gaaactggat ggagccaggc tgagcttgcc
     2161 cgtagaattg gtgtgacaca acaaactgtt caacaatggg tcagcggtaa ggctacacct
     2221 aaagcctcaa gtttggataa actggttgag gtttcagggc atccattgca ttggttttta
     2281 ttgcctcctg aagagtgtga gcaaattttc acccctgaca cgatgaaaat tggtcctcgc
     2341 caacgcgaac tgctccaagc ttttagtgcg tttcctgagg aagaccaaga aaaaatgctt
     2401 caagaaatca aagacaagaa aaaatcaatg gaagaaacca ttgctaggtg gctggcggca
     2461 caaaaaagcc gccgggcgtg accacagtac aagaagagga gttatgccat gggtacagcc
     2521 ctttctccga tagtttcaga attcgaaact accgaacaag aaaacagtta caacgaatgg
     2581 ttgcgcacta aagtaacgtc aagccttgca gacactcgcc ccgcaattcc acatgacgag
     2641 gtaatggctg aaatggaaaa tcttattgct caaattgctg taactaacaa gagcgagtaa
     2701 tgttacccat tttatggcta ccgtctgctc gcgatgattt gcgccagatc ataacttaca
     2761 tcgccaagga gaacccaccg gcagcacgta gactaaaaat acgcattgaa acatcggtat
     2821 tacctctatc tgagcatccg tacttatatc caccaagcga acgggtttct ggattgagag
     2881 agatcgtgac ccaccctaac tacataatcc tgtacagagt agctgcttca agcattgaga
     2941 ttgtaagcgt gacacattct cggcgacaat ttcccttctc tatctgagtt gaacaatttt
     3001 cataccccct ctttcgaggg atttttttgc ccaaaacaac aattaaagac tgttgacaca
     3061 aaacagtttt taattgtaga ttgcatacac caaccaccca ccccgcccca cagaacgccg
     3121 gacaatactt cgagttacca agcagtggtc agggggtaag tagccagccc gaggcgtatg
     3181 aacatgacgg cagggttcaa ctttaataac tatgcagcag gtttttgttc cgctacccgg
     3241 cgttaagggg aaatgaggtc aacatggata ctatcgatct tggcaacaac gaatctctgg
     3301 tgtacggcgt gtttcccaac caggacggta cattcaccgc gatgacgtat accaaaagca
     3361 aaacgtttaa aaccgaaaat ggtgcccgtc gctggctgga aagaaactca ggtgagtgat
     3421 atggatttcg acacaatcat ggaaaaggct tacgaagaat acttcgaagg ccttgccgaa
     3481 ggcgaggaag ctctcagctt cagtgagttt aaacaggcgc tttccagttc ggcaaaatct
     3541 aacgactaac ggagttaaag atggaattta aagatttacc aaaagaaatc caggaaattg
     3601 cagcacatgc acttcaccaa cgtctgaacg aagttgaatt agaatctgca acgaagaaat
     3661 acattgataa tatggctcgt aatgtgcgcg atgcgtttac cggattgtac tctgtttcgg
     3721 taacaaacaa ccagaatact gaagaaactg caaagcggat tgcctcggtg atgggttttc
     3781 atgtcgagga aaagtattca aaaaaagaat tctggaaaac tacaaaaaaa ttgcagagcg
     3841 agaactgtca tctcctgcgg caatcgcttc tttctatgag gaaagttatt cagatgacac
     3901 aggattaccg gaactgctct cattacttga aaaattcgga attatcatga aagggactgc
     3961 gtcggcggat ttcacatact caatgatggc tgctgatgga gagccttttc ctcccgaact
     4021 taaaataacc cccggcaatc atccgatggt aacaataaga gcaatagaga attactggtt
     4081 taatccagtt caacaatagt tggtctttgc aatattttta atgggccact acgcccttaa
     4141 tctcgggcgg cgtttaactc tttattcagt aaatcagact gatactgatt tggctctgta
     4201 atgatactgt caagttttga tatatgaaat tcaacatgtt ctggacagtt aaaaatgtaa
     4261 taaccgccct tctctaaaaa gccagaaaga gcgttacagg cagggcatgg tttgtttggc
     4321 attttatcct ccattaaggg ctgaattaaa aatggagacc aacacgctgc tacgtgtggt
     4381 cgtgcgccgg acacagataa gaatccggca ctgacagttt actgaaagga tatatccctg
     4441 aaaagtcagg gcataacacg aaagtgcacg gcgaagtcct tctcccttag agccgtcgtt
     4501 aaatttaatt cgaccgtgcg cttccggttg tggcaatccg cgaaatggcg cggcggtaag
     4561 tatggcgggg ttattctttc cccgttgagg acaccgggtt gtcaggttga ccatacgctt
     4621 aagtgacaac cccgctgcaa cgccctctgt tatcaatatt ctggtgacat ttggcggtat
     4681 cagttttact ccgtgactgc tctgccgccc ttttttaaaa gtgaattttg tgatgcggtg
     4741 aatgcggctc agcgcacgcg gaacagttaa atcggtaaag cggtcatttg ctgagtaacg
     4801 agaatgctct gtatccggcg ttaattgtta actggttaac gtcacctgga ggcaccaggc
     4861 accgcatcac aaaattcatt gttgaggacg cgataatgga aaagttatca tgtaatgcca
     4921 gcacgtctga acttcgtttc gaaattggcg ttatcactgg agacaaaaca tttattgaag
     4981 acgccattaa gcagagaaaa ctcgagcagg acctgttaaa tgaagtatgc attccttcaa
     5041 tgctggctcg tctggacctg ctgcaaaaag gatataaaca atgaatacaa catttgcact
     5101 cgttctgaca gtttatcttg tttccggcga atctcttgag ctggtgactg gcttatacgg
     5161 ttcaatgaaa gaatgcatgg ctgcagcagc agaacaaaaa attcccggta actgttatcc
     5221 ggtagataaa actactcaca ctaataataa cgaaataccg gcaggacttt aaaacagcac
     5281 cgtaattaat atccggtttc atttttatat gccagcaatg gcagggattt gttcaccctt
     5341 aaatctgtaa tgaggttaaa acaaaatgag taaagtcttt atttgcgccg ccattccgga
     5401 cgaacaggca ataaaagaag agggcgcagt tgctgtagcc actgccattg aagccggtga
     5461 tgaacgccgc gcccgtgcca aatttacctg gcaattcctg gagcaatatc ctgctgctca
     5521 ggactgcgct tataaatttc ttgtctgcga agataaaccc ggcatgcccc gccctgctat
     5581 cgactcctgg gataccgaat atatgcagga aaaccgctgg gatgaggaat ccgcttcctt
     5641 tattccggtc gaaccagaat ccgatcctat taacgtcaat tttgacaagc tgtcccttga
     5701 agtacagaac gcggtcctgg ttaagttcgg tacatgtgaa aacatcaccg ttgatatggc
     5761 gattgacgcg caggaattac tgcaagaaga cgtggctacc tttgacgggc atatcgttga
     5821 agcactgatg aaaacgcctg aaattaacgc tatgtatccg gaacgcaaac tgttcgctat
     5881 cggatgggtt aaacacaaat gtaagccggg taccaaatgg cccgaaattc aggctgaatt
     5941 acgtaactgg aaaaaacggc aggacgcaga gcgcaaagag actggaaaat acacgtctgt
     6001 tgttgatctt gcccgcgcca aagccaaccg acagcacact gaaaacccag cagaaaaaat
     6061 ccctcctgta actgccgcca ttcatcgcga atacaagcag acatggaaaa ccctggacag
     6121 ggagctggcc tactatctct ggcctagtga tgcggatgcc ggaaacattg acggcagcat
     6181 ccttcgctgg gctaaaaatg aagttatcgc cagagatcgc gaagactgga agcgcatctc
     6241 cgcatcaatg cgcaaacaac ctgatgcgct tcgctacagc cgccagacta tttttggcct
     6301 tgtccgtgaa cgtccgatcg acattcacaa agaccctgtg gcactgaaca aatacattac
     6361 tgaatacctg actacaaagg gcgtgtttga agatgaagga agaaatcaga gcgcaactga
     6421 tactctctcg tcgccagtac cagaaactga tgcagtggaa acggcaattc cggacaacga
     6481 aaaaaacgaa tgcaaagtgg aagtcgaacc atctgtagag cgtgaggggc cgttctactt
     6541 cctcttcacc gacaaggatg gcgaaaaata cggtcgcgca aacaaacttt ctggtctgga
     6601 caaggcgctg gctgccgggg ctactgaaat cacgaaagaa gaatatcttg cccgcaaaaa
     6661 cggaacatac acggacttac cgcaaaatgt ggataccgct gaagattccg tacaaccgga
     6721 gccggtaaaa gttaccgctg acgaagtaaa caaaattatg caggcagcca atatcagcca
     6781 gcctgacgcc gataaattgc ttgctgcatc acgtggtgaa tttgttgaag ggattagcga
     6841 tccgaatgat ccgaaatggg tgaaggggat tgaaacctgc gattctgtga accagaacca
     6901 gtcagaaacg gaacagaacg accaaaaagc ggaacaaaac agcccaaatg cgttacaaga
     6961 cgagccagaa acgaaacaat ccgaaccagt agcgcaacag gaagaggaaa aagtctgtac
     7021 cgcctgcggt cagagtggtg gcggcaactg ccctgattgt ggcgcggtga tgggcgacgc
     7081 aacctgccag gaaacattcg atgaagaaaa tcaggctgaa gctcaggagg aaatggaagg
     7141 cagtggcggc gatcactacc acaccacaga taatgaaagt ggcgagacag caaatccctt
     7201 aattaaggtg aacggtcatc gtgaaattac atccaccagc aggttgtggc accatctgat
     7261 gattgacctt gaaacaatgg gaaaaaatcc tgatgccccg attatctcaa taggtgcaat
     7321 atttttcgat ccgcaaaccg gagagatggg gccagaattc agcaaaacta tcgatctgga
     7381 aactgctggc ggagtcattg atcgggacac cattaagtgg tggctgaaac agtcacgcga
     7441 agcgcaatcc gccattctta ccgatgaaat cccgttagat gatgcactgc tgcaattacg
     7501 ggaatttatc gacgaaaact ccggtgaatt ttttgttcag gtctggggta acggtgcaac
     7561 tttcgacaac gtgattttac gccgttcata tgaacggcag gggatcccct gcccatggcg
     7621 ttacaccaat gatcgcgatg taagaacgat ggttgctctg ggactggtga tggatttcga
     7681 cgcaagaacg actattccat tcgaaggtga acgccataac gctctgaacg atgcacgtta
     7741 ccaggcgaaa tacgtttcag ccatctggca aaaactgctc ccgagtcagg ctgatttttg
     7801 atgttcaacc catatcgccg cccaccagct atagtggcgg cggtcatgct gtaaaggcac
     7861 gtgaccacat gtacgaatta actctatctc cagcagagat taaagagatc acgaaatacg
     7921 agcgatacac aaaacagcaa caccagttaa gactgcacgg catcccattt gtaatcggcc
     7981 ctaaaaacga acccatagtt ctccgcaggg atattccaca cggtctgaca acgatgccaa
     8041 aaacatctga actggtttct gctgaacccg attttgaggc gctgaacaat gggaagacca
     8101 agaaaaaata aaaaagataa tgtactgcca ccgcgggtta gatcgaatgg ttacagttac
     8161 gtgtggaaac c
//
LOCUS       DAZKHC020000157         8153 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZKHC020000157 DAZKHC020000000
VERSION     DAZKHC020000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.157"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(317..1510)
                     /gene="tyrB"
                     /locus_tag="ACHOSM_RS21995"
                     /old_locus_tag="pgaptmp_004399"
     CDS             complement(317..1510)
                     /gene="tyrB"
                     /locus_tag="ACHOSM_RS21995"
                     /old_locus_tag="pgaptmp_004399"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HGU4560763.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKVVAEAEVRLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGSDFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1763..2842)
                     /gene="alr"
                     /locus_tag="ACHOSM_RS22000"
                     /old_locus_tag="pgaptmp_004400"
     CDS             complement(1763..2842)
                     /gene="alr"
                     /locus_tag="ACHOSM_RS22000"
                     /old_locus_tag="pgaptmp_004400"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGU4560764.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2895..4310)
                     /gene="dnaB"
                     /locus_tag="ACHOSM_RS22005"
                     /old_locus_tag="pgaptmp_004401"
     CDS             complement(2895..4310)
                     /gene="dnaB"
                     /locus_tag="ACHOSM_RS22005"
                     /old_locus_tag="pgaptmp_004401"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU4560765.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4393..5376
                     /locus_tag="ACHOSM_RS22010"
                     /old_locus_tag="pgaptmp_004402"
     CDS             4393..5376
                     /locus_tag="ACHOSM_RS22010"
                     /old_locus_tag="pgaptmp_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HGU4560766.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5542..5784)
                     /gene="pspG"
                     /locus_tag="ACHOSM_RS22015"
                     /old_locus_tag="pgaptmp_004403"
     CDS             complement(5542..5784)
                     /gene="pspG"
                     /locus_tag="ACHOSM_RS22015"
                     /old_locus_tag="pgaptmp_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HGU4560767.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5918..6955)
                     /gene="dusA"
                     /locus_tag="ACHOSM_RS22020"
                     /old_locus_tag="pgaptmp_004404"
     CDS             complement(5918..6955)
                     /gene="dusA"
                     /locus_tag="ACHOSM_RS22020"
                     /old_locus_tag="pgaptmp_004404"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HGU4560768.1"
                     /translation="MHDNHETLKINQTSVMPEKTGVYWNSRFSIAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            7044..8141
                     /locus_tag="ACHOSM_RS22025"
                     /old_locus_tag="pgaptmp_004405"
     CDS             7044..8141
                     /locus_tag="ACHOSM_RS22025"
                     /old_locus_tag="pgaptmp_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4560769.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGTQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKAGRTKRYVLELLMDS
                     DISAIKLSELTENDVIEHCRLRNNAGAGPATVSHDVSYLGSVLDAAKPVYGINYTSNP
                     AKSARPYLLKLGLIGKSNRRNRRPASDELDMLIEGLQQRSTHKCSKIPFVDILKFSVW
                     SCMRIGEVCRLRWEDLDQEQKSILVRDRKDPRKKEGNHMKVALLGEAWDIVQRQPKKS
                     EFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDLRREGASRLFEAGFSIEEVAQVTGHRS
                     LNVLWQVYTELYPKSLHNRFEELQRSRNKTS"
BASE COUNT         2138 a         2097 c         1986 g         1932 t
ORIGIN      
        1 taattgaatc tcagaaagtc acaggcaata aggcaaagca tattcttgag gatatagaaa
       61 aattctatcg taacaaaccc gatacttatt attaaattta ataatggtta gatgtgcaga
      121 aattattact ccacccaaaa aaacaggata aggaattcca gataattagg ataagcttaa
      181 caataaaaga actaataacg tttattatct ctcacgtaga acgatggcat caaaaaaatc
      241 atctataaag aaagagtgaa agcgacgaca gtttaatttc actgcaggct gggtagctcc
      301 agcctgcttt cctgcattac atcaccgcag caaacgcctt tgccacacgt tgtacatttg
      361 ccgtatttaa cccggcgaca cacatgcgac cgctggcgat gagatagaca ccaaattctt
      421 cacgtagtcg gtcaacctga gcggcactta aaccggtata actgaacatg ccgcgctgat
      481 taagcagata atcgaaattg cgttctggca tctctgtgct taataccttc accagttcct
      541 gacgcattgc cagaatgcga gtacgcatct cttctacttc cgccagccag ctggctttca
      601 atgcctcgtc attcagcact gcagccacca cctgcgcacc aaaattcggc gggctggagt
      661 agttgcggcg aactgttgct ttcaattgcc ccagtacgcg gccagcggct tcggcatctt
      721 cacacataac agaaagtccg ccgacgcgct cgccgtaaag ggagaaaatt ttcgagaacg
      781 aattgctcac cagagcgggt aatccagcgc tggcaatggc gcgaatagcg taggcatcct
      841 cttccatacc ggcaccaaat ccttgatagg caatatcgag gaatggaata agctcgcggg
      901 ctttgagaat ttcaatcacc gcatcccact gatcattagt gagatcggca cccgttgggt
      961 tgtggcaaca tggatgcagc aacacaatac tgcgggcagg taatgttttc agcgtcgcca
     1021 acaggtcatt aaagcgcacg ctgttagtcg cttcgtcata ccaggggtaa gtactcactt
     1081 cgaatccagc cccggcgaat attgctacgt ggttttccca ggtaggatcg ctgacccaga
     1141 cgcctgattc cgggaagtag cgtttcagga aatccgagcc cactttcaat gcccctgagc
     1201 cgccaagggt ttgaatggtt gctacgcgct gttgttgcag tactggatgg tccgcaccaa
     1261 acagcagcgg cgcaatggca tggcgatagc tgttaagccc ttccatcggt aaataaagcg
     1321 aagcgccatg aggctgcgca ttcaggcgca cttccgcctc cgccacgact ttcagttgtg
     1381 gaataattcc gtcttcgttg tagtacagac cgatacttaa attcactttg tcgctgcgag
     1441 ggtcttcttt aaaacgctcc ataagcgtaa gaatcgggtc gccagcgtag gcgtcaactt
     1501 tttgaaacac gcgatggttc tccaggttta ggggcaggtg gttaaaacac aataaaccgg
     1561 aagaaggcga agatcgagtg gatgttcagg agcgaacggt aattagcaac agagtgagac
     1621 tcatgacaaa cgtacattcg ccagaggctc gacctttata agagcatgaa agaatatcaa
     1681 cttattgaat tggtaggatt ttattggccg gacaaggcat tcactccgca tccggcacag
     1741 acaaacaaat attacagaac gattaatcca cgtatttcat cgcgaccctt gaagtcaggc
     1801 gagtgataag ttcgtaagcg ctcacttttg tcatttcagc gatacgttct acgggcaaac
     1861 cttcgcccca taaaatgacc ggatccccgg ctttgtcctg cgcttgtgga cctaagtcta
     1921 cgcagatcat atccatcgcc acgcgcccga caatcggtac ttcgcgaccg ttcaccagca
     1981 ctggcgtacc ggacggcgcg gcgcgcggat aaccatcgcc atagcccatc gcgactacgc
     2041 caagacgggt atcacgttcg cttacccagg ttccaccata accaacaggc tctccggctt
     2101 tatgctcacg cacggcaatc aggctggagg ttagtgacat cactggctga cagccaaaat
     2161 cggcaccggt ggagcgatct tccagcggcg agacgccata aagaatgatg cccgggcgca
     2221 cccagtcaaa atgcgactgt ggccacagca gaatgccacc cgacgcggca atggaacgtt
     2281 gaccaggttt gccttcgcaa aaggtattaa agatagcgag ttgtttctcg gttgcgccac
     2341 attttggttc atccgcgcgc gcaaaatggc tgacgatatt caccggctga cgaacgtttt
     2401 tgcactgggt caggcgatga taaaacgcct cagcctgttc cggccttacg cccagacggt
     2461 gcataccggt atcgagtttc atccagacgg taaccggctc gtccaggcta gcctcttcca
     2521 gcgcagccag ctgttcttcg ttatgcacgg cggtatgaaa atgttgcgca gaaatcgtcg
     2581 gcagatctct ggcatcaaaa aagccttcga gtaacagtac aggtttggtg attccccccg
     2641 cacgcagtcg cagagcttct tcgagacggg ctacgccaaa ggcgtcagca tcggggagcg
     2701 ttcgcgcggt ctcaagaaga ccgtgaccat aagcgttcgc tttcaccacc gcaaccattt
     2761 tactggcagg ggccagttca cgaagacgtt gcaggttgtg tcgcagagcg cggcggttaa
     2821 tcacaacagt tgccgcttgc atttgtgttc cttgataagt gtttgcttta attacctaat
     2881 tcataaaata attattattc gtcgtcgtac tgcggccccg catagttgtc gaagcgcgac
     2941 cattgaccgt taaaggtcag gcgtaccgtc ccgattgggc cgttacgttg tttaccgata
     3001 ataatttccg cgatgccttt taaatcactg ttttcgtgat acacctcatc acgatagata
     3061 aacatgatca agtccgcatc ctgctcgata gagccagatt cacgcaggtc ggagttgacc
     3121 gggcgtttgt cggcacgttg ttccagagaa cggttcaact gggacagcgc caccaccggc
     3181 acgttcagtt ctttcgccag tgctttcagc gagcgagaga tttctgcaat ttccagcgta
     3241 cggttatcgg aaagcgccgg tacgcgcatc agttgcaggt agtcgatcat gataagcccg
     3301 atgccgccgt gttcacgggc aatacggcgt gcgcgggaac gcacttccgt tggcgtcagg
     3361 ccggaggagt catcgatata gatattgcgt ttttcgagca aaatacccat ggtgccggaa
     3421 atgcgcgccc agtcttcatc atcgagctgc ccggtacgga ttttagtctg gtcaacgcgc
     3481 gacagcgacg ccagagaacg catcataatc tgttctgaag gcatctccag cgagaagata
     3541 agtactggtt tatcctgcaa catcgccgcg ttttcgacga ggttcatcgc aaatgttgtt
     3601 ttacccatcg acggacgcgc ggcgacgatg atcaaatccg acggctgcaa gccagcggtt
     3661 tttttgttga gatcgtcata accggtgttt actccggtaa cgccatcgtg tggctgctga
     3721 aacaactgct caatacgcgc cacggttgcg tcgagcacat cggcgatgtt cttcggccct
     3781 tcgtctttgt ttgcacgact ttcggcaatt ttaaagacgc gggattcagc aaggtccagc
     3841 agatcttcgc tggtacgccc ctgcggatca aaaccggctt cagcaatctc attcgcaacc
     3901 gaaatcatct cacggacaac ggcacgttca cgcacgatgt ccgcataggc actgatgttc
     3961 gccgcacttg gcgtattttt tgacagctct gccagataag caaaaccacc gacgctatcg
     4021 agttgcccct ggcgttccag cgattccgca agggtaatca gatcgatagg actaccgctt
     4081 tcctgcaaac gcgccatttc agtaaagata tggcggtgtg ggcgggtgta aaaatcgtct
     4141 gccaccacac gctcggctac atcgtcccag cgttcgttat ccagcattaa accgcccaac
     4201 accgactgct ccgcttcgat cgagtgcgga ggcactttca gcccggcaac ttgtggatcg
     4261 cgttcgcggg gttcagcctg ctgtttgttg aagggtttat ttcctgccat agtgaatgga
     4321 gttaccgaga taaagaatgg gtcgaaactt taccatatga agcagaccct gacgatacgt
     4381 tctggaggac acatggcaac acgaattgaa tttcacaagc acggtggccc ggaagtactt
     4441 caagccgtag agttcactcc tgccgatccg gcagagaatg aaatccaggt cgaaaataaa
     4501 gccatcggca tcaattttat cgacacgtat atccgcagcg gcctttaccc gccgccatca
     4561 ctacccagcg gattaggtac cgaagcggca ggcatcgtga gtaaagtcgg cagtggtgta
     4621 aagcatatta aggcaggcga tcgtgtagtc tatgcgcagt cggcgttagg cgcttacagc
     4681 tctgtgcata acattaatgc ggataaagcg gcgattctgc ctgcggcaat ttcttttgag
     4741 caagctgcgg catccttcct gaaaggctta acggtttatt atctgctgcg caaaacctat
     4801 gaaattaaac ccgatgaaca gttcctgttc cacgcagcgg ctggcggcgt tggcttaatt
     4861 gcctgccagt gggcaaaagc cctgggcgcg aaacttatcg gcaccgtagg aaccgcgcaa
     4921 aaagcgcaga gcgcgctaaa agcgggcgcg tggcaggtta ttaactatcg tgaagaggat
     4981 ctggtcgagc ggttaaaaga gatcaccggc ggtaagaaag tgcgcgtggt gtacgattcc
     5041 gtgggcagag acacctggga acggtcgctg gattgcctgc aacgccgcgg cttaatggtc
     5101 agttttggca actcatcagg tgcggttacc ggtgtgaact taggcattct caatcaaaaa
     5161 ggctcgttgt atgtgacacg cccttccctg caaggctata tcaccacgcg ggaggaatta
     5221 accgaggcca gtaatgaact gttctctttg attgccagcg gtgtgattaa ggtcgatgtc
     5281 gccgagcagc agaaatatcc gctgaaggat gcgcagcgtg cgcatgagat tctggaaagc
     5341 cgggcgacgc aaggttccag cctgttgatt ccataaaaga aatagggctt ccacctggga
     5401 agccctttct ttttatagtt cggctgtatg tagggtacag cacgatgaat ctgttagagg
     5461 cgcaatagtg acagatttga ttatcaattc ctattttgtt ctaaggataa aaccttaggt
     5521 tgtgatcatc cgcacaatcc cttagtaacg ccagcggtca taacgctgat atttcggcac
     5581 ttttggtgct ttaatcgcct taataaccca caccaccgca atcgccagta gtaaccacgg
     5641 cagcaactta atcatcaatg ccagcatacc gccgaggaac ataatggccg tcgccacaac
     5701 cagcgcggcg ataatgccca gcaacgaaac gccggtgacc atcagcatga caaaaaagcc
     5761 aatcacaaaa agtagttcca gcatgatgct ctcccaaata tgaaatctct tgctggcatt
     5821 acaagaatca tgccaaaaat aatctattga tttaacagca aaacgccccg cgacggtgcg
     5881 cagggcgtgg tgaatttgac tactttttgg tgaaaagtta acgcttatcc gccaccagtt
     5941 tgagcgcgtg ttccagcaca ttaatgtctg cacccgcttt atgggcattt tcacttaaat
     6001 aacgccgcca ctgccgcgcg ccaggaatac cctggaacaa gcccaacata tgccgggtaa
     6061 tatggccgag atacgtcccc tggctgagtt cacgctcaat gtacggatac atggcgcgca
     6121 ctaccgccac cggatcggca tcggtatccg aggaaccaaa gatctcccgg tctaccgccg
     6181 ccagaatacc cggattctga tacgcctcgc gcccgaccat cacgccatcc atatgttgca
     6241 ggtgtgcttt ggcctcttcc agcgacttga taccaccgtt aatcgacatt gtcagatgcg
     6301 gaaagtcacg cttcagttga tacacacgcg gataatcgag cggcgggatt tcacggtttt
     6361 ctttcgggct taaccccgaa agccaggctt tacgtgcgtg gatgatgaac atctcacact
     6421 cgcctttgcc ggaaacggta ttgatgaaat cgcagagaaa ttcatagctg tcctggtcgt
     6481 cgatgccaat acgcgttttc accgtcaccg gaatcgacac cacatcgcgc atcgctttca
     6541 cgcagtcggc aaccagctgc gcattaccca tcagacacgc accaaacatg ccgttctgca
     6601 cccggtcaga cgggcagccg acattcaggt tgatctcatc atatccgcgc gcttctgcca
     6661 gctttgcaca ctgcgccagc gccgccggat cgctaccgcc cagttgcaac gctaccggat
     6721 gttcttcttc actgtacgcc agataatcac ctttaccgtg aataatcgcc cctgtggtca
     6781 ccatttcggt ataaagcaac gtattgcggg aaagcagacg caagaaatag cggcaatgtc
     6841 tgtccgtcca gtcgagcatg ggagcaatgc taaaccgaga attccagtaa acaccagttt
     6901 tttcaggcat cacgctggtt tgattaattt ttagtgtttc atgattatcg tgcatttttg
     6961 aacatttcag gctatttttc tcgcgttagg ttcccgcaca ggttcccacg ttttatggga
     7021 acccgaaata acgaggtcgt gtaatggcgt actataacat agagaaacga ctaaaatccg
     7081 atggcacacc acgctatcgc tgtaatgtga ttatcaaaga aaaaggtgtt atcacttaca
     7141 gggaaagcaa aacattccct aaacatgctc atgccaaaac atggggcaca cagaaagtga
     7201 tggaattaga tctatatggc attccatcat caaatgcagt tgacggactt acagtccgtg
     7261 acttactaca caaatattta aatgacccaa atgccggagg taaagcaggc cgtactaaaa
     7321 gatatgtgct ggaactgctt atggatagtg acatctccgc gatcaaacta tctgaactga
     7381 cagaaaatga cgtaattgaa cattgcaggc taagaaacaa cgctggtgca ggtccagcta
     7441 cagttagcca cgatgttagt tatcttggca gtgttctgga tgctgccaaa cctgtatatg
     7501 gaattaatta cacatcaaac ccagcgaaaa gcgctcgccc atatctactt aaacttggtt
     7561 tgattggtaa atcaaaccgt cgtaatcgta gaccagcatc tgatgaactg gacatgctca
     7621 ttgaaggcct tcaacaacga tctactcata aatgctcaaa aattccgttc gttgatatcc
     7681 tcaaattttc tgtgtggtcc tgtatgcgga tcggagaagt atgccggtta cgatgggaag
     7741 atctcgacca ggaacaaaaa tctatactcg taagagacag gaaagatcca cgcaaaaagg
     7801 aaggcaacca catgaaagta gccttgcttg gggaagcctg ggatatcgtc caacgacagc
     7861 ccaaaaaatc ggaattcatt tttccatata acagcacttc tgttactgca ggatttcaga
     7921 gggtaagaag caaattaggt attaaagatc tgcgatacca tgatttgcgt agagaagggg
     7981 caagtcgctt atttgaggct ggttttagta ttgaggaagt cgctcaggtt acagggcatc
     8041 gttcattaaa cgtgctatgg caggtatata ccgaactgta tccgaaatct ttacataatc
     8101 gttttgaaga gctccaaagg agcagaaata agacctcttg acactgttta tcc
//
LOCUS       DAZKHC020000158         8043 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZKHC020000158 DAZKHC020000000
VERSION     DAZKHC020000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.158"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            5..556
                     /gene="ygiL"
                     /locus_tag="ACHOSM_RS22030"
                     /old_locus_tag="pgaptmp_004406"
     CDS             5..556
                     /gene="ygiL"
                     /locus_tag="ACHOSM_RS22030"
                     /old_locus_tag="pgaptmp_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGU4560770.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQSDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            616..3120
                     /locus_tag="ACHOSM_RS22035"
                     /old_locus_tag="pgaptmp_004407"
     CDS             616..3120
                     /locus_tag="ACHOSM_RS22035"
                     /old_locus_tag="pgaptmp_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4560771.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVIIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLSTLINNKRTPIHLDLQWVLIDNLTAVCVTPEQLTL
                     LGFTDEFIEKTQQTLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAMNAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSHDFDLGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLCWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3136..3885
                     /gene="yqiH"
                     /locus_tag="ACHOSM_RS22040"
                     /old_locus_tag="pgaptmp_004408"
     CDS             3136..3885
                     /gene="yqiH"
                     /locus_tag="ACHOSM_RS22040"
                     /old_locus_tag="pgaptmp_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4560772.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3887..4951
                     /gene="yqiI"
                     /locus_tag="ACHOSM_RS22045"
                     /old_locus_tag="pgaptmp_004409"
     CDS             3887..4951
                     /gene="yqiI"
                     /locus_tag="ACHOSM_RS22045"
                     /old_locus_tag="pgaptmp_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4560773.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTTVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFAYFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(4994..5194)
                     /gene="glgS"
                     /locus_tag="ACHOSM_RS22050"
                     /old_locus_tag="pgaptmp_004410"
     CDS             complement(4994..5194)
                     /gene="glgS"
                     /locus_tag="ACHOSM_RS22050"
                     /old_locus_tag="pgaptmp_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HGU4560774.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            5463..6083
                     /gene="yqiJ"
                     /locus_tag="ACHOSM_RS22055"
                     /old_locus_tag="pgaptmp_004411"
     CDS             5463..6083
                     /gene="yqiJ"
                     /locus_tag="ACHOSM_RS22055"
                     /old_locus_tag="pgaptmp_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HGU4560775.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWP"
     gene            6119..7780
                     /gene="yqiK"
                     /locus_tag="ACHOSM_RS22060"
                     /old_locus_tag="pgaptmp_004412"
     CDS             6119..7780
                     /gene="yqiK"
                     /locus_tag="ACHOSM_RS22060"
                     /old_locus_tag="pgaptmp_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HGU4560776.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
BASE COUNT         2413 a         1727 c         1742 g         2161 t
ORIGIN      
        1 tattatgtct gcttttaaaa agtcacttct tgttgctggt gtagcaatga ttctttccaa
       61 taacgtgttt gctgatgaag gtcacggtat tgttaaattt aaaggtgaag ttatctctgc
      121 tccatgttct attaaaccag gtgacgaaga tttaactgta aatctgggtg aagtagcaga
      181 taccgtactg aaaagcgatc agaaatcttt ggcggaacct ttcactattc acctgcagga
      241 ctgcatgctg agtcagggtg gaactactta ttcaaaagcg aaggttactt tcactacagc
      301 aaataccatg acgggtcagt ctgatcttct gaaaaatacc aaagaaactg aaatcggcgg
      361 cgccactggc gttggcgtac gtattctgga cagccagagc ggcgaagtaa ctttaggtac
      421 tccggtagta attacgttca acaataccaa cagctatcag gaactgaatt tcaaagctcg
      481 tatggaatcc ccaagcaaag atgccacccc gggcaacgtt tacgctcagg ctgactacaa
      541 gattgcttac gagtaatcgc atttggccgg gttcacccgg ccacatcatt aacggattaa
      601 tgataagtgg atcagatgta taaaaaatta aaattaacca caataagcga attgattaaa
      661 aatatttatt gttcattatc cgttatcatc attggttgtg cgtcagctta tgccgttgaa
      721 ttcaacaaag atttaatcga agccgaagat cgtgaaaacg ttaacctttc ccaatttgaa
      781 actgatggcc aattacccgt cggcaaatat tcactaagca ctctgattaa taataagagg
      841 acgccaatcc accttgacct ccaatgggta ttaattgata acctaactgc agtttgcgtg
      901 acaccagagc aattaacatt attaggattt actgatgaat tcattgaaaa aactcagcaa
      961 accctgatcg atggttgtta ccctatcgaa aaagaaaaac aaattacaac ttatctcgat
     1021 aaagggaaaa tgcaattatc catatctgca cctcaggcat ggttaaaata caaagatgca
     1081 aactggacgc ctcctgaact ttgggatcat ggtattgctg gagcatttct tgactacaat
     1141 ttatatgcct ctcattatgc accacatcag ggcgataatt cgcaaaatat aagttcctat
     1201 gggcaggctg gggttaatct tggggcctgg cgcctgcgta ctgattacca gtacgatcag
     1261 tcatttaaca atggcaaaag ccaggcgaac aacctggatt ttccgcgtat ttatttgttt
     1321 cgcccaatcc cagcaatgaa tgcaaaacta actataggtc aatacgatac tgaatcctct
     1381 attttcgact ctttccattt ttctggcgtt tcgttgaaaa gcgatgagaa tatgttgccg
     1441 cctgatctac gtggttacgc accgcaaatc acgggtgttg cacaaacgaa tgcaaaggtc
     1501 actgtctcac agaacaaccg tattatttat caagaaaatg ttcctcctgg cccatttgct
     1561 attactaatt tattcaatac attacagggg caactggacg tcaaggttga agaagaggac
     1621 gggcaagtca cgcaatggca agttgcatct aatagtattc cttatctgac gcgtaaaggg
     1681 cagattcgct acaccactgc tatgggtaaa ccgaccagcg ttggtggtga ttccttacaa
     1741 caacccttct tctggactgg tgaattctca tggggttggc tgaacaatgt atccctgtat
     1801 ggtggttcag ttttaacaaa ccgtgattat caatctctgg ctgccggcgt tggctttaac
     1861 cttaactcat tgggttcatt atcttttgat gtcacacgat ctgatgctca gttgcataat
     1921 caggataaag aaacgggtta tagctaccgc gctaactatt caaaacgttt tgaatctacc
     1981 ggtagccagc tcactttcgc tggttaccgt ttctctgata aaaactttgt gacaatgaat
     2041 gaatatatca atgacactaa ccattacacg aattatcaga atgaaaaaga gagttatatt
     2101 gtcacgttta accagtatct tgaatcatta aggttaaata catacgtaag tttggctcgt
     2161 aatacttact gggacgccag cagtaatgtg aattattcat tatcacttag ccacgatttt
     2221 gatctcgggc cattaaaaaa cgtctccact tctctaacat ttagccgaat aaactgggaa
     2281 gaagacaacc aggatcaact gtatctaaat atttcgattc cctggggaac cagtagaaca
     2341 ttgagctatg gtatgcaacg aaatcaggat aacaagattt cgcatactgc ttcgtggtat
     2401 gactcttccg atcgaaataa ttcctggagc gtttctgctt caggcgacaa tgatgaattc
     2461 aaagatatga aagcgtcact acgcgccagt tatcagcata ataccgagaa tggtcgtctc
     2521 tatctctccg ggacatcaca gcgagacagt tattattctc tgaatgccag ttggaatggt
     2581 tcattcacgg cgactcgtca cggagccgct ttccatgact atagtggtag tgctgactcg
     2641 cgttttatga tcgacgcaga tggtgcagaa gatattccgt tgaacaataa acgcgcggta
     2701 actaatcggt atggcatcgg agttattcca tcagtcagca gttacataac aacatcatta
     2761 agcgttgata cccgaaatct tccagaaaat gtggatatcg aaaactcggt tatcaccacc
     2821 accttaaccg aaggtgctat tggctacgcc aaacttgata cccgcaaggg ctatcaaatc
     2881 atgggggtta ttcgcctggc agatggtagt cacccaccac tggggattag cgtaaaagat
     2941 aaaaccagcc ataaagaatt aggactggtt gctgatggcg gctttgtata cctcaacggc
     3001 attcaggatg acagcaaact tactttatgc tggggtgata aatcctgttt tattcaacca
     3061 cccaatagca gcaacttaac caccggaacg gttattttac cgtgtattag ccaaaattaa
     3121 ttcaggatga aactaatgcg ctacttaaac acaaagaatc tcattgcagc aggagtatta
     3181 ctcgcctgca tgagcagcat tgcctggggt gccatcatcc cggaccgtac ccgtatcata
     3241 atgaatgagt cagataaagg ggaagcatta aaacttacta atcaaagcaa aaatctaccc
     3301 tatctggcgc aaacatggat agaagatacg aaaggcaata aatcacgtga ctttattgtc
     3361 acggttcccc ctatggtacg tttaaatccc agtgagcaaa ttcaaataag aatgataagt
     3421 caggaaaaaa ttgctcaact gcctaatgac agagaaacgc tcttctattt taacgtgcga
     3481 gaaataccgc caaaaacaga taaaaaaaat gtgatgcaag tcactatgca gcacgcattg
     3541 aaactattct ggcgaccaaa agctattgaa ttagaagatg atggggtaat gtcttacgaa
     3601 aaagttgaaa tcatccgtag aaatgatggt tcgatccgct ttaacaataa gatgccttat
     3661 cacgttacgc tgggttatat cggaacaaac ggaatcacaa tgttgccaca aacgcaaagt
     3721 ttaatggtaa ccccttatag ccatgcagat acgcagttta aaaatgttcc gtcagctttt
     3781 caggttggtt atatcaatga ttttggtgga ttaagttttt atgaaataaa ctgcccgacg
     3841 gttaataact cttgtaatgt ttccgtagcc aaacgagata aataatatgc gctacttatt
     3901 aattgtaatt atatttatta taggttttag tgcattacct gtatgggcaa tggactgcta
     3961 tgctgaacat gaagggggaa ataccgtagt cattggttac gtaccaagaa tttctatccc
     4021 cagtaatggt aaaaaaggtg ataagatctg gcaaagcagt gagtatttta tgaatgtctt
     4081 ctgtaataat gcattacctg ccccatcacc tggagaagaa tacccatcag cctggactaa
     4141 tataatgatg tttttaccag gcggtcagga cttttataat cagaactcct atatttttgg
     4201 cgtaacatac aatggagttg attatgattc gactgcccct aatgctatcg cagcaccaga
     4261 atgcattgat ataaaaggag cgggtacctt taataatcac tataaaaacc ccgctgtctg
     4321 tagtggaggt cctgagcctc aattatcagt aacctttcca gctcgcgtac aactctatat
     4381 taaattagca aaaaatgcca atagagttaa taaaaacctg gtgttacctg atgaatatat
     4441 cgctctagag tttaaaggta tgagtggcgc aggagctata gaagtagaca aaaatttgac
     4501 cttcaggatt cgtggattaa ataatattca tgtccttgac tgctttgtta atgtcgcact
     4561 ggaaccagct gatggcgttg ttgactttgg taaaataaat tcccgaacaa ttaaaaatac
     4621 caccgttagt gagacgttta gtgtagtcat gacaaaagat cctggtgcgg cctgtactga
     4681 gcaattcaat attttaggaa gttttttcac tacggatatt ttgagtgatt atagccatct
     4741 ggatatgggt aatggactgc tattgaagat atttcataaa gatggaacag caacggaatt
     4801 taaccgcttc tcacaatttg cttatttttc atcgtctagt gcaccttcgg tcaccgcacc
     4861 attcagggca gaacttagtg cgaacccggc agagactgtt gttgagggac cgtttagtaa
     4921 agacgtaatc ctcaaaatca cctataacta gcatctaata caaacactaa aacgggccat
     4981 caggcccgtt atatcagtgc tctaactcca gctctcttgc ctgcatcatc tgttggcaat
     5041 accaggcata acgtgcgcaa aaccaggttc tatgttcttc atccatgtta ccagtaacgg
     5101 caagaatatc gaccgggcga ccttctgcgt gcattctggc aaaactacgc gccaggaaat
     5161 cgaaattatt taaagaatta agactatgat ccataagcac atcctccggt tatcgcatac
     5221 cttttaaggc catatgcttt ttctgatttc agtattcccc aatcagtgta gtgcctttaa
     5281 acataacgtg cgtaaatata ttgcccccga tcacactttt tagtgaaaaa taaaaaacca
     5341 tattaaaaaa ctttctcttc cagaacttct ctaacatgtg ttattattaa caaagataaa
     5401 tgtaagcaag gaaactatcc caaagtaaat attgcgtcac taaatggaca ttggagtgac
     5461 atatgatttt attcgccgac tataataccc cttatctttt cgctatttcc tttgtactgt
     5521 tgatcggcct tttagaaatt ttagcgctta tctgcggtca catgctctcc ggtgctcttg
     5581 atgcacatct tgaccattat gattccataa ccacaggtca tattagccag gcacttcatt
     5641 atctcaatat cggaagattg ccagctctcg tcgttctctg cttgctggct ggtttttttg
     5701 gtcttattgg catcctgttg cagcatgcct gcatcatggt ctggcaatca ccactgtcga
     5761 acctgttcgt ggttcctgtc agtttactgt ttacgattat tgcggtgcat tataccggta
     5821 aaatagttgc gccctggatt ccacgcgacc atagttcagc tattacagaa gaagaatata
     5881 ttggtagcat ggcattaatt accggtcatc aagccacttc aggtaatccc tgcgaaggaa
     5941 aactcaccga tcaatttggc caaatacatt atttactact ggagcctgaa gaaggaaaaa
     6001 ttttcactaa aggagacaaa gtgttaatta tttgccgact ttctgcaacg cgatatttgg
     6061 cggaaaacaa tccctggcca taaatattgt aatttattaa ttaaaaggaa atttaataat
     6121 ggatgatatt gttaattctg tgccctcctg gatgtttacc gcgattattg ccgtatgcat
     6181 tctgtttatt attggaatta ttttcgccag gctctatcgt cgcgcttcgg cagagcaagc
     6241 ttttgttcgt actggtttag gtgggcaaaa agtggtgatg agcggtggcg caatcgtgat
     6301 gccgatcttt catgaaataa tccccatcaa tatgaatact ctgaagctgg aagtcagccg
     6361 ctcaaccatt gatagcctga ttacgaaaga tcgtatgcgt gtcgatgtag tagtcgcttt
     6421 ctttgtacgg gtaaaaccgt ctgtagaagg catcgccact gcggcgcaga cgttggggca
     6481 acgcacgcta tcaccagaag acttacgtat gttggttgaa gataaatttg tcgatgccct
     6541 ccgtgcaaca gctgcacaaa tgaccatgca tgagttacag gatacccgcg agaacttcgt
     6601 gcagggagta caaaatactg tcgcagaaga cctgtcgaaa aacggtcttg agctggaaag
     6661 cgtttcactt accaacttta accagacctc gaaagaacat ttcaatccga acaatgcctt
     6721 tgacgccgaa ggtttaacca aactgactca ggagacggag cgccgtcgtc gcgaacgtaa
     6781 cgaagttgaa caggatgtag aagttgcggt gcgtgagaaa aaccgtgatg cactgtctcg
     6841 gaagctggag attgaacaac aagaagcgtt tatgacgctt gagcaggagc agcaggttaa
     6901 aacccgtact gccgaacaga atgcacgtat tgcggctttt gaagctgaac gtcgtcgtga
     6961 agcagagcag acgcgaattc tggctgaacg acaaattcag gaaacggaga tcgagcgcga
     7021 gcaggccgtc cgctcaagaa aggttgaggc tgaacgtgaa gttcgtatta aagagatcga
     7081 acagcagcag gtcaccgaaa tcgccaacca gacgaaatcg atcgctattg ccgccaaatc
     7141 ggaacaacag tcccaggcag aagcgcgtgc taaccttgca ctcgcagaag cagtaagtgc
     7201 gcaacaaaac gtagaaacca ctcgccagac tgccgaagcc gatcgtgcca agcaggtggc
     7261 gctgattgcc gcagcacagg atgcagaaac caaagcagtt gaactgaccg tgcgggcgaa
     7321 agcagaaaaa gaagccgcag agatgcaggc ggcggctatc gttgagttag ccgaagctac
     7381 acgtaaaaag ggtctggcgg aagcagaagc acaacgtgcg ctgaacgatg ctatcaacgt
     7441 actttctgat gaacaaacca gccttaaatt caaactggcc ttgttgcagg cgctacctgc
     7501 ggtaatagaa aaatccgttg agccgatgaa atctattgac ggtatcaaga ttattcaggt
     7561 cgatggtctg aatcgtggca gcgctgcggg tgatgcaaac acgggtaatg tggggggcgg
     7621 aaacctggcg gaacaagcat tatcagccgc tctctcttac cgcacacagg caccgctgat
     7681 tgactccttg ctcaatgaaa ttggcgtttc aggcggctca ctggcggcat tgacttcacc
     7741 cttaacctca acaactcccg tcgccgaaaa cgtagaataa attttcagct atgaacgtcc
     7801 ctggcacaaa aagccgggga ttttttatat ctgcgttccg ctaaaaggtg caaatgctca
     7861 ggacgttgca gcgttttgcg tgaccgctcg gggaaggcaa aactgcttca gggaaagcgt
     7921 tgcgcggggt ccggcgctca tcaacaatcg gggagtcagc aaggggctga aacgggaaag
     7981 cccctcccga ggaaggggcc ttacagaatg aaagggttat gatgaagctc gtcatcatac
     8041 tga
//
LOCUS       DAZKHC020000159         7893 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZKHC020000159 DAZKHC020000000
VERSION     DAZKHC020000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.159"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(188..451)
                     /gene="cedA"
                     /locus_tag="ACHOSM_RS22065"
                     /old_locus_tag="pgaptmp_004413"
     CDS             complement(188..451)
                     /gene="cedA"
                     /locus_tag="ACHOSM_RS22065"
                     /old_locus_tag="pgaptmp_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HGU4560777.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            634..2895
                     /gene="katE"
                     /locus_tag="ACHOSM_RS22070"
                     /old_locus_tag="pgaptmp_004414"
     CDS             634..2895
                     /gene="katE"
                     /locus_tag="ACHOSM_RS22070"
                     /old_locus_tag="pgaptmp_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HGU4560778.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTVPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYQSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKVLK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3153..3911)
                     /gene="chbG"
                     /locus_tag="ACHOSM_RS22075"
                     /old_locus_tag="pgaptmp_004415"
     CDS             complement(3153..3911)
                     /gene="chbG"
                     /locus_tag="ACHOSM_RS22075"
                     /old_locus_tag="pgaptmp_004415"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HGU4560779.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEAMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3924..5276)
                     /gene="celF"
                     /locus_tag="ACHOSM_RS22080"
                     /old_locus_tag="pgaptmp_004416"
                     /gene_synonym="chbF"
     CDS             complement(3924..5276)
                     /gene="celF"
                     /locus_tag="ACHOSM_RS22080"
                     /old_locus_tag="pgaptmp_004416"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGU4560780.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5381..6223)
                     /gene="chbR"
                     /locus_tag="ACHOSM_RS22085"
                     /old_locus_tag="pgaptmp_004417"
     CDS             complement(5381..6223)
                     /gene="chbR"
                     /locus_tag="ACHOSM_RS22085"
                     /old_locus_tag="pgaptmp_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HGU4560781.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6231..6581)
                     /gene="chbA"
                     /locus_tag="ACHOSM_RS22090"
                     /old_locus_tag="pgaptmp_004418"
     CDS             complement(6231..6581)
                     /gene="chbA"
                     /locus_tag="ACHOSM_RS22090"
                     /old_locus_tag="pgaptmp_004418"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HGU4560782.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLAHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6632..>7893)
                     /gene="chbC"
                     /locus_tag="ACHOSM_RS22095"
                     /old_locus_tag="pgaptmp_004419"
     CDS             complement(6632..>7893)
                     /gene="chbC"
                     /locus_tag="ACHOSM_RS22095"
                     /old_locus_tag="pgaptmp_004419"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HGU4560783.1"
                     /translation="RLMPLTLAGAMFVLINNVFLSFGEGSFFYSLGIRLDASTIETLN
                     GLKGIGGNVYNGTLGIMSLMAPFFIGMALAEERKVDALAAGLLSVAAFMTVTPYSVGE
                     AYAVGANWLGGANIISGIIIGLVVAEMFTFIVRRNWVIKLPDSVPASVSRSFSALIPG
                     FIILSVMGIIAWALNTWGTNFHQIIMDTISTPLASLGSVVGWAYVIFVPLLWFFGIHG
                     ALALTALDNGIMTPWALENIATYQQYGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGA
                     TLGLILAIFIASRRADYRQVAKLALPSGIFQINEPILFGLPIIMNPVMFIPFVLVQPI
                     LAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFFNTNGSVAALLVALFNLGIATLIYLP
                     FVVVANKAQNAIDKEESEEDIANALKF"
BASE COUNT         2114 a         2072 c         1771 g         1936 t
ORIGIN      
        1 gctcatcatg attagcaaaa cttaaccatt ttaaaataaa taaacaatta aagaaaaaag
       61 atcacttatt tatagcaata gcacgtcaaa ggcagctttt tgttacaggt ggtttgaatg
      121 aatgtagcaa cgaaatgcag aatttcaggt catgtaactc ccggcaaaac cgggaggtat
      181 gtaatcctta ctcagtcact tccccttcct ggcggatctg atttgcccaa cgttgggcag
      241 attcaggcac agtaaacgcc ggtgagcgca gaaatgactc tcccatcagt acaaacgcaa
      301 catatttgcc acgcagcatc cagacatcac gaaacgaatc catctttatc gcatgttctg
      361 gcggcgcggg ttccgtgcgt gggacatagc taataatctg gcggttttgc tggcggagcg
      421 gtttcttcat tactggcttc actaaacgca tattaaaaat tagaaaaact gtagtttagc
      481 cgatttagcc cctgtacgtc ccgctttgcg tgtatttcat aacaccgttt ccagaatagt
      541 ctccgaagcg ggatctggct ggtggtctat agttagagag ttttttgacc aaaacagcgg
      601 ccctttcagt aataaattaa ggagacgagt tcaatgtcgc aacataacga aaagaaccca
      661 catcagcacc agtcaccact acacgattcc agcgaagcga aaccggggat ggactcactg
      721 gcacctgagg acggatctca tcgtccagcg gctgaaccaa caccgccagg tgcacaacct
      781 accgtccctg ggagcctgaa agcccctgat acacgtaacg aaaaacttaa ttctctggaa
      841 gacgtacgca aaggcagtga aaattatgcg ctgaccacta atcagggcgt gcgcatcgcc
      901 gatgatcaaa actcactgcg tgccggtaac cgtggtccaa cgctgctgga agattttatt
      961 ctgcgcgaga aaatcaccca ctttgaccat gagcgcatcc cggaacgtat tgttcatgca
     1021 cgcggatcag cagctcacgg ttatttccag ccatatcaaa gcttaagcga tatcaccaaa
     1081 gcggatttcc tctcagatcc gaacaaaatc accccagtat ttgttaggtt ctctaccgtt
     1141 cagggtggcg ctggctctgc cgataccgtg cgtgatatcc gtggttttgc caccaagttc
     1201 tatactgaag agggtatttt tgacctcgtt ggcaataaca cgccaatctt ctttatccag
     1261 gatgcgcata aattccccga ttttgttcat gcggtaaaac cagaaccgca ctgggcaatt
     1321 ccacaagggc aaagtgccca cgatactttc tgggattatg tttctctgca acctgaaact
     1381 ctgcacaacg tgatgtgggc gatgtcggat cgcggcatcc cccgcagtta ccgcaccatg
     1441 gaaggcttcg gtattcacac cttccgcctg attaatgccg aagggaaggc aacgtttgta
     1501 cgtttccact ggaaaccact ggcaggtaaa gcctcactcg tttgggatga agcacaaaaa
     1561 ctcaccggac gtgacccgga cttccaccgc cgcgagttgt gggaagccat tgaagcaggc
     1621 gattttccgg aatacgaact gggcttccag ttgattcctg aagaagatga attcaagttc
     1681 gacttcgatc ttctcgatcc aaccaaactg atcccggaag aactggtgcc cgttcagcgt
     1741 gtcggcaaaa tggtgctcaa tcgcaatccg gataacttct ttgccgaaaa cgaacaagca
     1801 gcattccatc caggtcatat tgttcctggt ctggacttca ccaacgatcc gctgttgcag
     1861 ggacgtttgt tctcctatac cgatacacaa atcagtcgtc ttggtgggcc gaatttccat
     1921 gagattccga ttaaccgtcc gacctgccct taccacaact tccagcgtga tggcatgcat
     1981 cgtatgggga tcgacactaa cccggcgaat tacgaaccga actcgatcaa cgataactgg
     2041 ccgcgcgaaa caccgccggg accgaaacgc ggcggttttg aatcatatca ggaacgcgtg
     2101 gaaggaaata aagttcgtga gcgcagtcca tcgtttggcg aatattattc ccatccgcgt
     2161 ctgttctggc taagtcagac gccattcgag cagcgccata ttgtcgatgg tttcagtttt
     2221 gagttaagca aagtcgttcg tccgtatatt cgtgagcgcg ttgttgacca gctggcgcat
     2281 attgatctca ctctggccca ggcggtggcg aaaaatctcg gtatcgaact gactgacgac
     2341 cagctgaata tcaccccacc tccggacgtc aacggtctga aaaaggatcc atccttaagt
     2401 ttgtacgcca ttcctgacgg tgatgtgaaa ggtcgcgtgg tagcgatttt acttaatgat
     2461 gaagtgagat cggcagacct tctggccatt ctcaaggtcc tgaaggccaa aggcgttcat
     2521 gccaaactgc tctactcccg aatgggtgaa gtgactgcgg atgacggtac ggtgttgcct
     2581 atagccgcta cctttgccgg tgcaccttcg ctgacggtcg atgcggtcat tgtcccttgc
     2641 ggcaatatcg cggatatcgc tgacaacggc gatgccaact actacctgat ggaagcctac
     2701 aaacacctta aaccgattgc gctggcggga gacgcgcgca agtttaaagc aacaatcaag
     2761 gtcgctgacc agggtgaaga agggattgtg gaagctgaca gcgctgacgg tagttttatg
     2821 gatgaactgc taacgctgat ggcagcacac cgcgtgtggt cacgcattcc taagattgac
     2881 aaaattcctg cctgatggga ggcggcgcaa ttgcgccgcc tcaaatgatt tacatagtgc
     2941 gctttgttta tgccggatgc ggcgtgaacg ccttatccgg cctacaaaac tgtgcaaatt
     3001 caatatattg caggaaacac gtaggcctga taagcgaagc gcatcaggca gttttgcgtt
     3061 tgtcagcagt ctcaagcggc gcagttacgc cgcctttgta ggaattaatc gccggatgca
     3121 aggttcacgc cgcatctggc aaacatcctc acttacacat cccgataact ccccaaccga
     3181 taaccacgct gagcgatagc acctttcaac gacgctgatg tcaacacatc cagctccgtc
     3241 aagcgtggga aacagtaagc actctgacgg atagtattat cgataaacgc cggatgacac
     3301 atcgcctcca gcgaacgatc accccgatgg cttgcgtcat ccagcacttg caggaacagt
     3361 gcctcgctaa tctcttcacc ataaaacgcg ctactgaatc cctgagaact gcgcagatta
     3421 gccggtaaat caccagcgtt agatagcggc tgacggtcga tgcgcaacgc aatcccctct
     3481 tcagccgcaa acctggcaac aatcgggaaa atctgcggga acatatgcac atgatgatgg
     3541 ctatcaagat gcgtaggttt gcgtccaaat agctcaatga aacgcaaata ctgactggca
     3601 agctcctgag taatttcttc cagcggtaaa gcatcttctt ctgccaactg ccagatccat
     3661 tttcccagca caccatcgcg ggttaacccc ggcatagctg tcagtggctt gcccatagta
     3721 aggacaaagt gcatccctat ggccagactc ggttcatcac gactcaactg caccgcatgg
     3781 tcaatagcct gcccattcac cagcgccgtc gtcgatgtga caatcccatt gcgacaggcc
     3841 tcgataatgc cgtagttctg acctttgctt aagccaaaat catcggcatt aacaatcagt
     3901 aagcgttcca taatcagcct cggttaatgt gcttttttaa gctctgcgat gcagtcggca
     3961 aaatttggca gccatttctc gtgcgccaga atcatctcgc gtgccagcag ctcagcatcg
     4021 cgatcggaat gcaccaacgg actaaggttt agcgccagta acacatcgtt aaattctccg
     4081 ctaagtgcgg cgttgctggc agcaatctcg aagcctttaa tggtgtgaat cagccccatc
     4141 actttatcat cgaaatgcgt aatgcgcgga tgtggcgtcg cgccatcgcg ccccagctta
     4201 caggtcattt ctaccgccca gtctgccgga atattatcaa tatgcccatg atgcgggata
     4261 ttaacgtaat gttcagcttg cttgtcgttg tagatagcgt tgatcacttc gcacgctgca
     4321 tcagagtaat aagccccacc gcgctgttcc agttctttcg gcttaacttt caactccgga
     4381 tttttataca gctcaaaaag ttgtttctcg actttctgta ctacctgtgc tcgtgcgccg
     4441 cctttgtagt attcgcccat ttcaatagcc agcatctctt tctgcttgaa gtaatacagc
     4501 agataagaac atggcagcag atttaacgaa cgaattaagc cctcactaaa tggcagatcg
     4561 aaaatatttt ttacagagga cgcttttaac tgccctgacg ccacaccatc aagcaattcg
     4621 gcaaagcgcg acttgccatt taccagcaca tccttaatga acaccatatg gttgaggccg
     4681 aacagatcga tagataaatc atcgctgtct ttcagcatca gaacatcgcg aataaacatc
     4741 ttcatgccga tcggaatatt acacacgccg ataaagcgtt taaatccggt atgacgataa
     4801 acggcttcag tgaccattcc cgccgggtta gtgaagttaa tcacccatgc attcggacaa
     4861 agttcttcga catcttttac gatgtcaaaa atcaccggaa tggtacgcag acctttaaac
     4921 agaccgcccg cgccgttggt ttcctgacca agataaccat gacttaatgg aatacgttca
     4981 tccagttcgc gcgccggtaa ttggcctacg cgcagttggg tagtaacgaa atcagcatct
     5041 ttcaatgctt cgcggcgatc cagcgtttta taaagcttca tcgggacgcc agcgttatca
     5101 atcatccgtt ggcagagttc aaaaatgata tccagtttct ctttaccatc ttcgacatcc
     5161 accagccata attcgctgac cggcaattcg tgataacgct taataaatcc ttccagtaac
     5221 tccggggtat agctgctccc gccaccaata gtgacgactt ttaatttctg gctcataatt
     5281 tctcccttca gtacagaata ctgatatctg gcatatctgc ccccccggac ataaataatc
     5341 cagcaacagg acagatatgt gaattgtcag gtataacgac ttactgatta aattcagtca
     5401 atttcttacg atagctctta ggcgtaaagg acgttaattt cttaaacgtt ttaataaaca
     5461 aactcgggct actataaccg gcctcaaacg caatatccgt cacggaatag ttggtcattt
     5521 ccagttgttt tttggcaaaa ttaatacgga tctcattaat aatctgcatt ggtgttttgc
     5581 cataatatcg ttgagtcgct cgcgtcaaat attcctgtga tttggctgac aacgtcacca
     5641 tattctccag cgccgattca ctaaactgct ctttatcatg catcttttct accgtacttt
     5701 tcagccactg cggtacatca tcaatcacct gttcttcgcg gtaatgacgt aaacggttaa
     5761 tgacataaaa agtaaccatc tcaacaaact cttccagccc tgtttcgcgg aaattcaatg
     5821 aagaaatcac tgtttccaca taggtgagaa aagcgttatt ggtccggtat acctgcgaag
     5881 cgacaaagca ataaggcaac aacggcaggt aatgctgctc aaaaaagcgt ttactgatcc
     5941 caacgttcaa tatacgcgtg gcaccaaact cataaaaact ttgatggtgc gaacctaacg
     6001 gaataaaaac aaaatcgccc cgttccagta acacgcgctt accgttaatc tcctggaaat
     6061 aacgcccggt taataccaga gtaaattcat aatagtcgtg ctgatgcagc ccgctgatac
     6121 tctcagtttt gttatagata aacacatgga agtttttgcc attaaacaac tgctgttctc
     6181 gggcagtggc aatttccggc gcgttaatca ctggctgcat catcgactcc ttatgccttc
     6241 agtttttcat gaagctcaat taattcagta atcagttcac gcgcaagcat ggacgtcatt
     6301 aaatgatcct gagcgtgggc cagcaccaga ctcactttca tcttaccttc gcccgcatcg
     6361 ccttcaatca gtttcgtctg taccagatgc gcttcattca atgccatccg tgactgatcc
     6421 atcatggctt ttgctgctgc aaaatcgccc tgcttcgcct gtttcagtgc cgcatacgcc
     6481 aggctgcgcg cttgtccgga gttgatgata agccccatca ccacttcttc cagctcttca
     6541 gcttctgttt gcgtatcagg aatattatcg agatccatca tacatcgtgc ctctctcttc
     6601 ttaccggcaa gattacccgt accggcatcg attaaaattt cagggcgtta gcgatatctt
     6661 cttcgctctc ttctttatca atcgcatttt gtgctttgtt agccaccaca acaaagggca
     6721 gataaattaa cgttgcgatg ccaaggttga agagtgcgac cagcaatgcg gcgacgctac
     6781 cgttggtgtt aaagaaggct cccagaccgg ttggcatggt ccacggtgca atattggtca
     6841 ccggaggaat gatgcccatg tagtacgctg cgagggtgat tgccgccaga atcggttgta
     6901 ccagtacaaa cgggataaac atcaccgggt tcatgataat tggcagacca aacagaatcg
     6961 gttcgttaat ctggaagatg ccggacggca gcgccagttt tgctacctga cgataatcag
     7021 cacggcgaga ggcgataaag atagccagga tcaggcctaa agtcgcacca ctgcccccaa
     7081 ggaaaataaa ggagtccagc atcggcttgg cccagatatg gaaggtctta ccggctgcca
     7141 gcgccgcttc gacggaacca tattgctgat aggtcgcgat attttccagc gcccacggcg
     7201 tcataatgcc gttgtccagt gcggtcagcg ccagcgcgcc atgaataccg aagaaccaga
     7261 gcagtggaac aaagatcaca taggcccagc ccaccacgct acccaacgat gccagtgggg
     7321 ttgagatggt atccataatg atctgatgga agttggtgcc ccaggtattc aacgcccagg
     7381 caataatccc catcacggaa agaataataa agccgggaat taatgccgag aaggaacgcg
     7441 atactgaagc aggtacgctg tcgggcagtt taatgaccca attgcggcgg acaataaagg
     7501 taaacatttc tgccaccacc aggccaataa taatcccgga gatgatattc gccccaccta
     7561 accagtttgc gccaaccgca taggcctcac cgacactata tggggtgacg gtcataaatg
     7621 ctgcaacgga taacaacccc gccgccagcg catcgacttt acgctcttct gccagcgcca
     7681 tgccaataaa gaacggtgcc attaaagaca ttatccctaa tgttccgtta tatacgttgc
     7741 cgccaatacc tttcaggcca ttaagtgttt caatggttga ggcgtcgagg cgaataccta
     7801 aggaataaaa aaacgacccc tccccaaagc ttagaaaaac gttgttaatt aatacaaaca
     7861 tggcccccgc aagggttaac ggcattaagc gaa
//
LOCUS       DAZKHC020000160         7797 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZKHC020000160 DAZKHC020000000
VERSION     DAZKHC020000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.160"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..145
                     /locus_tag="ACHOSM_RS22100"
                     /old_locus_tag="pgaptmp_004420"
     CDS             <1..145
                     /locus_tag="ACHOSM_RS22100"
                     /old_locus_tag="pgaptmp_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdhR family protein"
                     /protein_id="HGU4560784.1"
                     /translation="LFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQA
                     KLA"
     gene            271..1875
                     /gene="ydhS"
                     /locus_tag="ACHOSM_RS22105"
                     /old_locus_tag="pgaptmp_004421"
     CDS             271..1875
                     /gene="ydhS"
                     /locus_tag="ACHOSM_RS22105"
                     /old_locus_tag="pgaptmp_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HGU4560785.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEANHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLQLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSKTFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(1887..2699)
                     /gene="ydhT"
                     /locus_tag="ACHOSM_RS22110"
                     /old_locus_tag="pgaptmp_004422"
     CDS             complement(1887..2699)
                     /gene="ydhT"
                     /locus_tag="ACHOSM_RS22110"
                     /old_locus_tag="pgaptmp_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HGU4560786.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLAQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(2703..3488)
                     /gene="phsC"
                     /locus_tag="ACHOSM_RS22115"
                     /old_locus_tag="pgaptmp_004423"
     CDS             complement(2703..3488)
                     /gene="phsC"
                     /locus_tag="ACHOSM_RS22115"
                     /old_locus_tag="pgaptmp_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HGU4560787.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHDLLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLVCWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPSVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(3485..4152)
                     /gene="ydhX"
                     /locus_tag="ACHOSM_RS22120"
                     /old_locus_tag="pgaptmp_004424"
                     /pseudo
     CDS             complement(3485..4152)
                     /gene="ydhX"
                     /locus_tag="ACHOSM_RS22120"
                     /old_locus_tag="pgaptmp_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(4216..4854)
                     /gene="ydhW"
                     /locus_tag="ACHOSM_RS22125"
                     /old_locus_tag="pgaptmp_004425"
     CDS             complement(4216..4854)
                     /gene="ydhW"
                     /locus_tag="ACHOSM_RS22125"
                     /old_locus_tag="pgaptmp_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HGU4560788.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKVYGLCEWFEVEQFQNP"
     gene            complement(4867..6969)
                     /gene="ydhV"
                     /locus_tag="ACHOSM_RS22130"
                     /old_locus_tag="pgaptmp_004426"
     CDS             complement(4867..6969)
                     /gene="ydhV"
                     /locus_tag="ACHOSM_RS22130"
                     /old_locus_tag="pgaptmp_004426"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HGU4560789.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMNQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLISSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(6990..7616)
                     /gene="ydhY"
                     /locus_tag="ACHOSM_RS22135"
                     /old_locus_tag="pgaptmp_004427"
     CDS             complement(6990..7616)
                     /gene="ydhY"
                     /locus_tag="ACHOSM_RS22135"
                     /old_locus_tag="pgaptmp_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HGU4560790.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         2027 a         2041 c         1758 g         1971 t
ORIGIN      
        1 ccttttcact gatgaaaaaa gcgcccttgc ctatctggaa aaacacactg cccgactgaa
       61 aaatctcggc gttgaggaag ttgtcgccaa agttttcgat gtcaatgagc cactgagtca
      121 aatcaatcag gcaaaactcg cctgacagaa tttaatcaag ggcggttagc gcccttttca
      181 tccctgtctg aaatttctca aattctaaga atctcaacca aacttatctg ataacactaa
      241 attcgaaaga atgcgtacag gtaagtaaca atgaaaaaaa ttgctattgt gggtgccggg
      301 cctacgggga tctacacctt attctcgctt ctacagcaac aaactccact ttctatttct
      361 atcttcgagc aggctgacga ggccggtgtc gggatgccat acagtgatga ggaaaactca
      421 aaaatgatgc tggcaaatat tgccagtatt gaaataccgc cgattaattg tacgtatctc
      481 gaatggctac aaaagcaaga agccaaccat ctccagcgtt atggcgttaa aaaagaaacc
      541 ttgcacgatc gtcagttttt accgcgaatt ctactgggcg aatatttccg cgatcaattt
      601 ttacaattag tagaccaggc acgaaagcaa aaatttgcag tggctgttta tgaatcatgc
      661 caggttaccg atctgcaaat tacaaatgct ggcgtcatgc tcgctacaaa tcaggattta
      721 cccagcaaga cgtttgattt agcggtgatt gccacgggtc acgtctggcc tgatgaagaa
      781 gaagcaaccc gaacgtattt tcccagcccg tggtcaggcc tgatggaagc aaaggtcgat
      841 gcgtgtaacg tgggtattat gggaacatcc ttgagcggac tggatgcggc aatggcagtg
      901 gctattcagc atggttcgtt cattgaagat gataaacaac acgtcgtttt tcaccgcgat
      961 aacgcaagtg aaaagctaaa tatcacgttg atgtcgcgca cgggtatttt acccgaagcc
     1021 gatttctatt gccctattcc ctacgagccc ttacacatcg tcaccgatca ggcattaaat
     1081 gctgagattc aaaaaggtga agagggcctt ttggatcggg tatttagatt gatagtagag
     1141 gaaatcaagt ttgctgatcc agactggagc caacgcatag ccttagagag cctgaatgtc
     1201 gattcctttg ctcaagcctg gtttgccgag cgcaaacaac gcgacccatt tgactgggca
     1261 gaaaaaaatc tccaggaagt cgaacgcaat aaacgagaaa aacatactgt tccctggcgt
     1321 tatgtcattc tgcgcctgca tgaagccgta caggaaattg ttccacatct gaatgaacac
     1381 gaccataaac ggttcagtaa aggccttgcc cgggttttta tcgataatta tgcggcaatc
     1441 ccttcagagt ctattcgtcg gctgctggcc ttacgtgaag cggggatcat tcatattctc
     1501 gccctcggtg aagactacga aatggaaatt aatgagtcgc gcaccgtcct gaaaacggaa
     1561 gacaacagct actcgtttga cgtttttatt gatgcccgcg gacagcgtcc gcttaaagtg
     1621 aaagatattc ctttccctgg gctacgcgag caattacaga aaacagggga tgaaatccct
     1681 gatgttggcg aagattatac gttacagcaa cccgaagata ttcgtggacg cgtagcgttc
     1741 ggcgcgttgc cctggttgat gcacgaccag cctttcgttc agggacttac ggcatgtgca
     1801 gaaattggtg aggcgatggc tcgggcggtc gtaaaacctg catcccgtgc acgtcggcgt
     1861 ctttcgtttg attaaaggta aagctatcag cactcaacaa actgatgctg ttcattaaga
     1921 cgatagcgga ctccggctcg aatattgttt tcaccttcaa tggcgacggc gaagcgaact
     1981 ctttcgccgt catgatatgc cagtgcggcg cttccccctg gcccgagaat aattgagtcg
     2041 accacgcccg tgctcactac cgttgaattt tcaccgctgg caatgatgtg tacattgtcg
     2101 ccgctgttgg caattctggc gtttgcgcca aagctggcga tttgcgccag gtcaccatta
     2161 ctggcgacat gacaccgttc acccaatgta caaacgcgta ctcgcattcc ggtgttcgca
     2221 atccgactac tatctccagc gctgcttatc cgcgaagaat taccggttac agcaacccgt
     2281 gcgcgttcac cggagctgcc aatgtgactg ttaaagccga cacttccaat ccgcacgcta
     2341 taacccatac tggcaatttg cgcggcatac ccagcggttg cgattcgggc gttatcctca
     2401 caacagccga tgcgagcatg atcgtcgctg cgggcaaact gactgcaaaa tggtatctct
     2461 ccggggtctg tggccagttt ttgcatagag gaaacttcct gatgcgcgaa gttttcatcc
     2521 gccagccatt ttttccacgc atactcaacc agactctccg cccagtccgt atatccttcc
     2581 tcaatcagtg catgatgaat atcggcataa ctaccgccgc gtgggaaatg gcgcaggaac
     2641 cagcgataca tcaccgctcc gatacgccat tctcgcaggt ctgctcgtgt aatgatcatt
     2701 cgttagtgcc ggtgatagcc atcgaccatg cttttaaagg tttcgtgtgg cgtacgcccc
     2761 gtggtgcaaa gataaagatg accgaagata aaaaagaggc ttataaatgc cagagcaaaa
     2821 tgcgcctgca ataaccagta tcttacgcta ggaaacacat ctcccacggc ttgcggatag
     2881 agacacaaca gccccgtcaa tagtaacaac ggcagcaatc catacatgac accaacatag
     2941 gcgacctgct gtaagggatt aaatttagac tgtgttgttg ccgggaaagg atgttcttcc
     3001 ccctgcataa tgccaaacaa ataaaatcgc gtttgttttg ccgctcgttc cagccacccc
     3061 tgacgacgaa tgcgatagtg gtgaccatta tccccaacgg cattgatcag cacaaagccg
     3121 agccagcata ccagtaacaa aaatccgcaa acttcatgca ccgcaaccag acttttaacc
     3181 gcagttgcgc ccaccatcgc aaagtgattt atcagcccac ttgccagcaa caatacaaag
     3241 agtaacgcat tcgaccagtg ccataatctg accgctttgc tgtaaagata aaccttctca
     3301 ccatgatcgg tggctgattt cttcacgcct cgagcacgaa gcaagtcgtg cagccccagc
     3361 accaaccaca tcccaaccag caatactccg gcaatgatca accacaccgg ccaaaattcg
     3421 ggagtgaact gtggcacata gttcgctaac tggctctgaa actgttcagc atgttgcgac
     3481 gggttcatac attttccttt ttaatcaaat gttgaccgaa ccggcgatac agatgcggtt
     3541 tcccggcacc aggtagctga tattgatagt atttattatc ctgcaaccac gcctgaattt
     3601 ctgggctatc ttcgcgacca aagatcaatg cgtgttccgg acaggcgctg acgcaaatgg
     3661 gcggaaagcc ttttgccagt cgggactcag cgcagaaatc acatttatct gccactttag
     3721 tgacagggtt aaggtagcga acctggtacg gacaagcgcc aatgcaataa ctgcagccga
     3781 tgcactgcga tttttctacc cgcacgatcc cctgctcatc acgccacgat gccccgtcgg
     3841 gcatacgtcg atgcacggag catcttcgca gtgctgacat gactggcgaa aaaagtgata
     3901 ctgcgtttcg ttgtcgttat cggtgactgg aatgtgtgct atcgataaac ggcttccctg
     3961 agcggggacg tggttagttt tacggcatgc gcgggcgcaa atattacagc cattgcaccg
     4021 tgactcgtca tgaatcatgg cataccgaac ttccttttct tgcctcgtgt tcgctaacag
     4081 agaagacgca gaaccagtaa aaaagattac tgttcccatc cccagaacaa atttgcgtcg
     4141 agtgaaggac atctgattat cccctaatgc agtggctgca actgtccacc ctatccatga
     4201 catctccctg aaatttcagg ggttctgaaa ctgctctacc tcgaaccatt cgcataagcc
     4261 ataaaccttg ccataggtca taaatcgctc gctaaacaag tacaacaccg ccgtcgatga
     4321 ctccacctgc cgtatatcgg catattcagg tgcaacatcc atcgcggcga tcgcggcttc
     4381 aatctgcgct tcctggaagt aatatggcgc ttgcatcagc atggcgactt tgtaaggacg
     4441 aggatacgtc tggcactcaa aacgaacggc gtgtgcaatg gcgcgacaaa tgtcctgctc
     4501 cacaacttgc aaagacatcg ccgcgtagtt ttcactcatg gcctgcgttg agtaatagta
     4561 atcatcctga cttcccgtca tacaagcgat atccgcgtag tctccgttct gcttaatgct
     4621 ttccagcaaa actgacagtt cctcttctgc aaccgaatag ggtggcgaga gaaacacggc
     4681 gcgcgccacc agctgcccag ccgcagaatg ctggcgaatg aactctgcca ggatttcagc
     4741 cggttctggt tcggttaccg tatcaacggg gtgacgcatc ccctgtaacc attgccgctt
     4801 cgcctcgtca acttcgctga ctttcgctaa cggcaattca tcccgatggt tcatctcacc
     4861 cattccttac actggcagta gattgtgtgc tgccagatcg gcggcaatat cttccagccc
     4921 cagacgctgc aatgtttcgc gggttggaca accaagctgt gggtcccagc ccatttcttt
     4981 gtagaacatc gtgagcgaag catgcatatc gtcacgatcc attttgtcag taccttcagt
     5041 aaagaccggg atctgcggat ccttgtcgaa tacccaggaa ctgataagat cgtgttcatt
     5101 acgcatatct ttggtttgca tcagttttac cgtgtaggca cgatgcaacg taaagatacg
     5161 ctctgcagct aaatctaatt tttcctgagt catctcttcg ccggtgatcg ctttgaagaa
     5221 tttggcttca agcgccagat cgccccggta attacggctt ttcagcggcg aaacggtcat
     5281 tggccagacc cagttacaca gggtgacggc gttatgcaaa cagacccgca acagcgacca
     5341 cttagcatat ttaatttttg cgtcattgat tggcgtgtag tttttggttt catcgtaagc
     5401 atcttcagaa ccaaaaagtt ctttcgccac ttcacgttgc agtttcaatg gcaagccgga
     5461 accaataaag ttgatatggg tatgcgtcat gcagtcacgg ttgaacatac agttaacaat
     5521 ggaaccgacc tgcgctgacg cttcattggc atggtgaacc ggatagccaa acggcgacca
     5581 gagtttattt ttcgcgtagc cccagtactc ttcccccaaa ttccagcgtt ctgcgatggc
     5641 atatgaacca tcagccaggt gactcagctc acccacacga tgcgccagac ggtagtaaaa
     5701 atctttaatg aagttaacgt cacccgcttc cagttgatcc cagcgaattt ctgcatactc
     5761 ttcagctggc agaacacgct tgaacacacc tttgctgtaa caataagtaa agtcgcgatg
     5821 caactgcccg tagttacacc ataggccgta gtcatcgaac aaattcagac caaccaggtt
     5881 accaatcaca cgaccatcgt ctttatcttc aaaatctttc ggcccgttcg ggaagatggt
     5941 ggtatggaca aagtttgcta cacaagtgtt accacctgtg ctgggcacgc caaactcttt
     6001 cacccgagga atattcattt ggttcataca acggatcggg caagagtggc agccgctcat
     6061 ttttactgtg tatttctctg ccgccggtcc taagtcaaaa acggatttat aggtacgaaa
     6121 gccgaccgta ttctgattgc ctggcggaat ttcacccgtt tcaatcggac caccttcagc
     6181 cgcgccccaa aacagccctt tacgtgctgt ccagcgtgac ttgggatctg aatactctgc
     6241 ccacgattgt ggcgtacttg gcacgacatg gttgttattc gcaccaataa gttcagtcat
     6301 catgtaatca ttgagacgct tcatctcctg acgatcggca atgttgaccc ctttcgtccc
     6361 ttcaaccgca atcgctttca ggtttttcga acccattatt gcgccagttc ccgcaccgcc
     6421 gctgtggtta cggctattca acatgccaga gagaggaaca aggttttccc cagcctgacc
     6481 aatagccgcc acacaggttt ccggactggt caatcgacaa atttcttccg tcgttgcgcg
     6541 cgtccctttt ccccataaga aatcggcttt ttccaggcta actttgtcat ctttaatctt
     6601 cagccatacc ggtgatttcg ccttcccttc gataataatg acgtcgtatc cagcgaattt
     6661 catttgcgct gcaaaaaagc cacccatatg ggcatcgacg actaaatttc ctttggtaaa
     6721 agtagaaagt gaggtgatat ttacgcgaga actacagggg gcaccagatc cagttaatgg
     6781 gccggtagca aagactaatt tattcgcttc atcgaaaggt ttcgtgcctg gcggtacttc
     6841 gtcatacata attttgtagc cgaagcccat gccaccgaca aaacttttaa acttactgga
     6901 atcttcgagg gtaatatttc ctgtcgtgag attgactctt aatatattac ctgtccaacc
     6961 gttagccatg attttttcct ttgcaagatt cacacagtaa tatctttcca ctcgataatt
     7021 tttaacgccc ctgttgggca ggcgtttgcg cattcaccgc ataacacaca tttcgaggat
     7081 tttttacttt cggtatttac ggtggccatc atccacgggc atgccgtagt acaggcgcta
     7141 cagccaatac agcgtttatg atcgacggta atacagcctt ctttctgctg ccaggtaatc
     7201 gcaccaatcg ggcagacgtt catgcattgc ggctctttgc attgacggca ggtgtccgcg
     7261 gtatagttga gatcgccata caggccgccg ccagagccaa ccccgttgtc gccaaagaaa
     7321 taattgcgat ggattttgat acgggagaag aatgtcccta ctgagccatc gttgaagttg
     7381 gtacaagaga tttcacagcg gtggcaaccc gtgcatcgcg ctcgctgggt caccagcacc
     7441 cctttgggtg tatttatcaa ccctactgtg ccgctatcaa tatcttcctg tttgcaaccc
     7501 aaaagtgaca gtaacgcagg ggcaattgtt agaccagcaa ggccttttcc tgatatacgc
     7561 aagaattcta atcgcgttaa gccaatatct aatagtggac gatcaaccgg gttcatttat
     7621 ttcgcatcct cttcgcaaac acagtgctga caattcagca gcctgatatt aagcattcaa
     7681 ctaatacgct gctgactctg tccccaacac cagatatttt ccctaacccg atagtgtatg
     7741 agaaaagatt taacgccgat aaactaatcc tttatggtta ggagaaaaaa taatgtg
//
LOCUS       DAZKHC020000161         7789 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZKHC020000161 DAZKHC020000000
VERSION     DAZKHC020000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.161"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHOSM_RS22140"
                     /old_locus_tag="pgaptmp_004428"
     CDS             complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHOSM_RS22140"
                     /old_locus_tag="pgaptmp_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGU4560791.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAVAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGA"
     gene            516..1217
                     /locus_tag="ACHOSM_RS22145"
                     /old_locus_tag="pgaptmp_004429"
     CDS             516..1217
                     /locus_tag="ACHOSM_RS22145"
                     /old_locus_tag="pgaptmp_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HGU4560792.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     DHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHOSM_RS22150"
                     /old_locus_tag="pgaptmp_004430"
     CDS             1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHOSM_RS22150"
                     /old_locus_tag="pgaptmp_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HGU4560793.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1616..2926)
                     /gene="cyuA"
                     /locus_tag="ACHOSM_RS22155"
                     /old_locus_tag="pgaptmp_004431"
     CDS             complement(1616..2926)
                     /gene="cyuA"
                     /locus_tag="ACHOSM_RS22155"
                     /old_locus_tag="pgaptmp_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HGU4560794.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2954..4285)
                     /locus_tag="ACHOSM_RS22160"
                     /old_locus_tag="pgaptmp_004432"
     CDS             complement(2954..4285)
                     /locus_tag="ACHOSM_RS22160"
                     /old_locus_tag="pgaptmp_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4560795.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4819..5349)
                     /locus_tag="ACHOSM_RS22165"
                     /old_locus_tag="pgaptmp_004433"
     CDS             complement(4819..5349)
                     /locus_tag="ACHOSM_RS22165"
                     /old_locus_tag="pgaptmp_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560796.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGESPNDVGICGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLDKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
     gene            5540..5788
                     /locus_tag="ACHOSM_RS22170"
                     /old_locus_tag="pgaptmp_004434"
     CDS             5540..5788
                     /locus_tag="ACHOSM_RS22170"
                     /old_locus_tag="pgaptmp_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4560797.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            5790..>7789
                     /locus_tag="ACHOSM_RS22175"
                     /old_locus_tag="pgaptmp_004435"
     CDS             5790..>7789
                     /locus_tag="ACHOSM_RS22175"
                     /old_locus_tag="pgaptmp_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HGU4560798.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSSDVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVMKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWRLRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKMKCRHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSMDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADVLDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSAMRDLPSWAELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRPDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVAVQQPAV"
BASE COUNT         2052 a         2061 c         1916 g         1760 t
ORIGIN      
        1 cgcccccgcc agcacttcgg tgatgattgc cagttgggta ttggcttttg ccgccagttt
       61 gtcgattaac gggaaaaagg caggtgtcgg taccagcgcc tcggtcacaa tggtgagatg
      121 cgtttcccaa ccgcgcgcga gggcttccgc atcggtagtc agtttatctg ctgcttccag
      181 caaaacgcgc cctcgctcca gcaacatcct cccgacattg gtgaatttgg tgcgatggcc
      241 cgagcggtca aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag
      301 tgcggaaggc acgcgtccca gctcatccgc cgccaccgca aaactgcccc ggcgatcgat
      361 cgcatccata acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc
      421 attcaggaaa tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc
      481 ttaccataaa aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc
      541 agtgtggaca agcagactac ggatggttgc aggcccggta tactttttcc tttggacact
      601 acttcgaccc gaaattgtta ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg
      661 ccccaggtgc cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc
      721 tggatggaga agcagagtat cgcgacagcg aaggcaatca tgttcaggcc agcgccggtg
      781 aggcgttgct gctctctacc cagccgggtg tcagctacag cgatcacaat ctcagcaaag
      841 acaaaccgtt aacacgaatg cagctttggc tggacgcctg cccgcagcga gagaatccgc
      901 tgattcaaaa actggcgctt aatatgggca agcagcaatt aatcgcctcg ccagagggta
      961 cgatgggaag cctgcaatta cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag
     1021 gcgaaagtgc gaatttccag ttgcatgggc cacgcgcgta tttgcaatcg attcacggga
     1081 aatttcatgc gcttacgcat catgaagaga aagcggcgct gacctgcggt gatggggcgt
     1141 ttattcgtga cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga
     1201 tagatttgcc tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcggccaaa
     1261 aagcgccagc cagtgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt
     1321 ggctatgaag agatgttgag cgagctggaa gccatcgtcg cggatgctga aacgcgttta
     1381 gccgaggatg aagctaccgc gtaaagacag gttgccggat gcggcgaaaa cgccttatcc
     1441 ggcctacaaa atccaacaac attaacgaat taaacacccc gtaggccgga taagatgcgc
     1501 cagcatcgca tccggcatca ggtacaaaac acaacaacat caacaagttg agcaccccgt
     1561 aggccggata aggtgcgcca gcatcgcatc cggcacaata ctcaaaacct ggcgtttatc
     1621 tggccttgct cgccataatc tcgataatct gccgatccgt ttgctgcatc gaatggcttg
     1681 ctaacgcaca caggttggca atcgactgct caacatcatg cgccacgatc ccttcattgc
     1741 cggtcacggc ggtatcatcc agcgccatta acaccgcttt ccacgcagcc gaagcactgg
     1801 tcgaaacctt catcgcgcag ctgttcgacg caccatcgca aatcatgccg ctgacatcgc
     1861 cgatcatact gctgatcgcc atcgagatag tttcataacg cccatccacc agccatgcca
     1921 tcccggcggc ggcccccatt gctgcggtcg ttgcggcaca aagtgcagac aaacgcggta
     1981 actggttatg gatgtaaatt gcgctcaaat gagaaagcat cagcgcacgc gccagtcgtt
     2041 catcatccgc tccgaagtgt tctgctacaa ccaccacagg cattgttgcg gtgatcccct
     2101 ggttacccga gccggagtta ctcatagccg gaagcgtagc gccgcccata cgcgcatcgg
     2161 atgccgcgct ggtacgaatc acaatggatg aagagagatc tttcgccagc aaaccgcgct
     2221 cgcactgttt ttccagcgtc gcgccaatat gcagccccca cttaccgctc aaaccttcct
     2281 gcgataacgc acaatttagc ttcgcggaat cgagaataaa gcggatcgcc gcaaacggga
     2341 cttcattgac gaacttcagg atctcagcca gcgtcgttct ggaaagcacc gtcagcggag
     2401 actcttgctc gccctctgcc acacacgcct gctgggtaaa caccacacca ttgtgcgtct
     2461 caatatgcac aatgttggta tgcccgccga cgatggtgac acacgcccac ttctcaccgt
     2521 tccagacttt ggcgcgtgag aagaggattt cattgcaagg ttcctggatc ttaacggaga
     2581 ctttccccgc cgccagcagt gctttggcat cggcaattgc ctgcgcagtt gcgtctttca
     2641 gcacttccag cccggcgttg gcatttccac ctaacgcccc cagcgccgcc gcaatcggca
     2701 gccccaccat tcccgtgccg ggaacggtga cgcccagacc gttcttcatc agatttggcg
     2761 aaacccaggc ttctacacgt tcaaccggac cttccagttc tgccgcagca accgccgccg
     2821 ccagcgccag tgaaatcggt tcagtacatc ccagcgccgg ttttacttcc tcctgaactg
     2881 caaggatgta acgctgccat aacggattta aagtcgaatc aaacatatta aaaaccttaa
     2941 aatttcaggt aaatcaagaa aatgccagga acggagaaac acaaagcaac aaaccagtga
     3001 cgataatcag gtacagagac atccctttgt atttgtgcaa tgccggtact ttgtaaacca
     3061 gccacgccgg gatcaggcac cctaccatgc cgaaaatcgg gctacagata gaggtgaagc
     3121 tcaacaccgg tgcgttcagt acgatggcgc tccaggccag caaaatggcg aaaatcatga
     3181 tgccgcgctg aacaagattt tcgttaatct tctcggcagg catcttgcga cgcaggatgt
     3241 tcattacgat cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag aatgcagtca
     3301 tgacagcaaa gatattgaga atgacgctga ccactttcac ccacgctgcg ccgtcaccgc
     3361 taataaactg tgcggcaatc gccagcgcgg aaatattctg ctcataggct tttaccgctt
     3421 cgtcatgtcc catcgccagc gtgaacgaca cggcgtagaa aaagacggtg acaaacaaaa
     3481 tgccaaacgc gatattcatt gcccgcaatg ctttatgccg cgccacttca attgattttt
     3541 cccgcgagcg ataagagatc accatcgggc ttaacgtctg gataaacaga atcgacgtca
     3601 gggtaaacgg cagcgtaata atggcgtttt tcaccagcag ccccagcggc ggtagcgaac
     3661 cgacgttata cagatgccac attcccacca tcgacacgcc cagcgccgcg accaccagca
     3721 gcttggtcag caccatgccg gtcgaaattt tgaataacaa tttctcgccg cgtgaggaga
     3781 tcgccaccag aatgcaaatc agtaccagac cataaaaggg actgtctgac agcaaccctt
     3841 ccgtcacgcc gaaggtatgc agataggaag cactatcgtt ggtgatggcg gtggaataga
     3901 cgaacatcca aatcaccagc attacgaaat agagcgcacc taacaggatg ccccagtttt
     3961 tacctaaata accgctaatg acactcgggt aatctttaca ttctggtgat tctgccagcg
     4021 tattaataaa caaccgctga aacagataca ttgccgggta accaatcacc gatgagagca
     4081 aaaataccca caatcccatc aaaccgacct gcaccgggag aaaaacaatc cccgcgccaa
     4141 tcgccatccc gatactcata atcacccagc cggtgtcagt actgtcgaat ttgatcgctt
     4201 ctcgccactc gctctcactc attccagcac gacccgccgg ggtcgaagcg tctgcaatga
     4261 cgcctttatt cgatgcaatt tccataattt ctcgctcaat attttgtagg gcttattatt
     4321 tttttccgag ccgcatcaag gcgatatgcg gtctacgttt ttgcaggcga gttattagaa
     4381 taaagaaatg atacgcgcga ggtcagagaa aatcttcaca atcaagactt tgaaataacg
     4441 gagatggata agaattttct actaaattaa tcgcaagaga atattcattc tctatttgcg
     4501 gcagatcaca aaaaaaaggt gcacatttgt gcacccaagg atgaaagctg acagcaatgt
     4561 cagccgcatg taaccaggtg aatcatttga taccaacgag ggttttagtg aaaggaggtg
     4621 aaatcacctg taagatactg aaataaaaca ggtgatgtct ggatgagcgc ttaaagtcta
     4681 tttgataccg attgtgcaat aattaatcgt ttattctaaa atcggcaaaa cttttgatac
     4741 tgcgatctca actttttaaa gcgtttaaaa cttcattaaa tctatatggc aactaacata
     4801 cgggccagcc tcgccaaact aacccgcttt agattctacc ggatttgatt gcctacttgt
     4861 ctcggttttt tccaaaatct ctctgacgca atcgtctggc atatctacaa tcatcaacgc
     4921 agccaggcgc ttcctgcctt ctagttgtaa taaatcctgg ttttctttat ccagcagtgt
     4981 cagaaccgtc tctacacctt tgcgagcaag gagattggtt aactgttctc gttcgtcttt
     5041 cgtaaggaat gaaagcatct gtgtaagttg atctccctta tcaccagaac aaccaaaatt
     5101 accacaaata ccaacatcgt ttggtgattc acccttgccc tctttaagcc actcaagact
     5161 aactccttca ctttcagaaa ttcgtataag gatactttct ctgggggctg gttgtgtttg
     5221 tcctcccttg aaatagctgt ttaatgtgtt gatattgatt ccccacgctc tggcagctgc
     5281 acttcttgaa gggtagcgct ttgtaagcat tccaatacgt tcaataacag gttcttttcc
     5341 ctgcgacata aagatacctc ttggtgcttt tctaaggaaa ggtttctttc tatttatcca
     5401 attgaaaaat aaggataaaa gcaaaaatta gccaaaatac caaaaatacc ttgatttggt
     5461 atttttggtg atgcattatt tacccgtgca gatatccgca cgaatatccg cgcgggtagc
     5521 tttttagaat agaggaagta tgagtagaag agaagtttca gatcacgatt ggccgcctga
     5581 aatcatcaag gcgcgcttac acatggctgg cttatcactg cgctctttat ctttaaaggc
     5641 tggttacagc agggattcgc tgaaaagcgt attacgtact ccttgccgac cgtatcagca
     5701 aataattgct gatgctcttg gggtatcgcc tgaagaaatc tggcccagta gataccaggt
     5761 taagagctat atgagaaagg cgtcatgata tgtatgtcat tgcaaaagaa ctgattggcg
     5821 cgcccggaat gcctgctaca acaaaaggta ttcgccaggc attacaacgt tacgtacaag
     5881 ggaaaagctg ttgttcccgt cgtcgctcag gctctaaagc aactgaatac agcatcgact
     5941 gtttacctga agtgacgcag caggcattac gtgaacgtta tgccctgcaa ctgatgacgc
     6001 aaaaagccga tgaatcaccg gctccggtgg tgacaaaggc cagacgctca tctgacgtgg
     6061 ttgatgcggt ggaggcatat cgcggatcac cacaactgat ggtcgaacgc ctcaatgccc
     6121 tgactgaaaa ccagcgccag gtggctgatg cacgaatcgc gatcgtcagc gaagtgatga
     6181 aagtcgcgca acaacccggt ttcagctgcg cgaaggctat ccggtttatc gttgacaacc
     6241 tggcacgttc acagctggac gagcgcattg tggcaatggt tgagacggcg aacgccaaaa
     6301 agggaaacag ccgtgcgttg agtgaaatca cgctgaagcg atggattgcg gcctttaaca
     6361 aggcacagaa cgccgctgaa cgcctgcttt tactggcacc gggtaaacgc caggaaataa
     6421 aagccgaaga tattaactgg ctgcccgaat ttctggcgca gtatcgccag tcaaacggcc
     6481 gaccaatgac cgaggcttac gaggattttg ttgctgaatg gcggctccgg cacgctgatg
     6541 agccttatat gctcgatatc atgccctctt atgacaccat tcgccgcgca atgaagaaac
     6601 tgccggaagt ggtgaaacaa aaaggccggg tgacaggcag tgaataccgt cagcttgagg
     6661 gattcacgcg ccgcgactgg tccagaatgc cggtgaatta tgtctggatt ggtgacggtc
     6721 acggcatgaa gatgaaatgc aggcacccgg ttcacgggcg gccatttgca ccggaagtga
     6781 cctttgttat cgacggtggc acgcgctttg tggtgggctg gagcatggac ctggctgaaa
     6841 atgttttcgc cgtagccggt gccatacagc acggtattcg ccatcacggc aaaccgtttc
     6901 tgtattactc ggataacggt tcgggggaaa ccgccgacgt cctggataag gaggttgtgg
     6961 ggatactgcc gcgactgggg attaatcacc cgaccgggat tgccggtaat ccgcaggggc
     7021 gaggcattat cgaacggctt aaccgcacat taccgatgcg catagcccgt aaataccgca
     7081 cctatttcgg gaaaggtgca gatcgcgaga cgttacgcaa aaccaaccgc gatttacgct
     7141 cggcatttac cgccctgcaa cagggcaaac ggctgaacgc ccggcaacag tcagcgatgc
     7201 gtgatttacc gtcctgggct gaactgattg atgccattcg tgacggtgtt gagtggtaca
     7261 acaaccggcc gcacgatgaa ttaccgatga agccgaacgg taaacattac agcccggcgg
     7321 agttcagaaa aaaacgcctg gcggaagagg acacggaaat tgaatggctg tccgatgttg
     7381 agctgcggga tatgttccgg ccgatggtgg aacgccctgt aagacgctgt gaaatacgct
     7441 ggctgaataa tatttactac gcgcccgagc tgcgtgacga gcatggtcgt aaggtgctta
     7501 tcagctatga cattcatgat gccgaacgaa ttaccgtacg tcgcccggat ggcagcgtga
     7561 tttgcgaggc ggtatgggac ggcaataaac gcgaagcctt cccggtcagc gcggaatact
     7621 acaaacagca gcagcgcctt aaaggtatgc gtaaacgcgc agaggaaaaa atccgtgatg
     7681 ccgaggatga ggttgtcaac gtgctggagc acaagccgca ggagccatgg ctggaaaaca
     7741 tctaccgccc ggtgggtaat acggtggccg ttcagcaacc tgccgttga
//
LOCUS       DAZKHC020000162         7751 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZKHC020000162 DAZKHC020000000
VERSION     DAZKHC020000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.162"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(488..1141)
                     /locus_tag="ACHOSM_RS22180"
                     /old_locus_tag="pgaptmp_004436"
     CDS             complement(488..1141)
                     /locus_tag="ACHOSM_RS22180"
                     /old_locus_tag="pgaptmp_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU4560799.1"
                     /translation="MMNMSDRIRQRRKELNLTQQALADLTGVNRVTVTGWEKDDYQPN
                     GANLQALANALKCDPLWLVSGKGSPEPKINLKPEIFAVKKVPLISWVQAGSWTMTEPG
                     VRKEDAEEWVYTTALVSEMAFALRVRGDSMTNPLGSPSIPEGSIVIVEPDIIDTECIN
                     GKIVVAHINGGQEATLKKFVEDWPNRYLVPLNPNYKTIECGENCRIVGLVKQVIMDF"
     gene            1237..1434
                     /locus_tag="ACHOSM_RS22185"
                     /old_locus_tag="pgaptmp_004437"
     CDS             1237..1434
                     /locus_tag="ACHOSM_RS22185"
                     /old_locus_tag="pgaptmp_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HGU4560800.1"
                     /translation="MRKSDVINYFGGVCKTAEALGIKHPSVSEWPEIIPEGRAYQLEK
                     ITNGKLKVDVSLYQKTNSAAA"
     gene            1462..2046
                     /locus_tag="ACHOSM_RS22190"
                     /old_locus_tag="pgaptmp_004438"
     CDS             1462..2046
                     /locus_tag="ACHOSM_RS22190"
                     /old_locus_tag="pgaptmp_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfL family putative regulatory protein"
                     /protein_id="HGU4560801.1"
                     /translation="MGKEPEWKVDKQPAWLVAAIRRTIADLPHGYEEAAEILGLYKSD
                     DITPAKDQLHNRLRSGGDQIFPLEWAMVLQDASGTRHVTDAIARRSNGVFVPLVVIDD
                     IDNGDINQRLMESIEWIGKHSQYLRKATADGVIDQAEREQIEENSYQVMAKWQEHLTL
                     LFRVFCAPEKSNARECAAPGVVASIASGCGETNA"
     gene            2192..2287
                     /locus_tag="ACHOSM_RS22195"
                     /old_locus_tag="pgaptmp_004439"
                     /pseudo
     CDS             2192..2287
                     /locus_tag="ACHOSM_RS22195"
                     /old_locus_tag="pgaptmp_004439"
                     /inference="COORDINATES: protein motif:HMM:NF025339.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            2241..3206
                     /locus_tag="ACHOSM_RS22200"
                     /old_locus_tag="pgaptmp_004440"
     CDS             2241..3206
                     /locus_tag="ACHOSM_RS22200"
                     /old_locus_tag="pgaptmp_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4560802.1"
                     /translation="MLFVGSQKHSALSIFAKTTRMSALVLCGNSGVILLSVKDHQHID
                     SAIPGRYTVQAPYKAGAGIGVLKLITEHNRAHAVFSCHEHCYAQIMVGRTGPTSVGPG
                     SVVSGNANPVRLTTSVIGVPCGEFSEKLTTGAVTMTTLPTLSQPEIAIVDGQAVTSSL
                     AVADFFSKRHDDVLKKIRILDCSPEFCARNFAETSILVHQPNGGTRKLPCYHITRDGF
                     AFLAMGFTGKRAAQFKEAYINAFNQMEKQLSKPSVPSDVAHNASVLYSYVSSIHQVWL
                     QQLYPMLEKAESPLAVSLYDRINDAAFLARLIHSSLNSSEVRGRK"
     gene            3203..3427
                     /locus_tag="ACHOSM_RS22205"
                     /old_locus_tag="pgaptmp_004441"
     CDS             3203..3427
                     /locus_tag="ACHOSM_RS22205"
                     /old_locus_tag="pgaptmp_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560803.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRVSMPLIAFRSGKNMKHLGGAA"
     gene            3424..4416
                     /locus_tag="ACHOSM_RS22210"
                     /old_locus_tag="pgaptmp_004442"
     CDS             3424..4416
                     /locus_tag="ACHOSM_RS22210"
                     /old_locus_tag="pgaptmp_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560804.1"
                     /translation="MSLLMTSQPIVINSDLACRIGLNEAIVLQQLHYWLNETNSGTEH
                     GGIRWVYNTTEQWLEQFPFWSESTLKRTFASLKSLGVLRREQLNKSKRDMTNFYTINY
                     ESELLEEVKVNESIRSKCTSPSGQSDLMDGRKMTRSIGSKRHAVIGSKWPNDLTENTT
                     EITTENKTSSRPDASQPDTQTAEQEFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKK
                     IIALYEQAAECDGEVVRPKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWC
                     RNVLSPSKLREKWDELSLRLSPSVSTHTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            4413..4496
                     /locus_tag="ACHOSM_RS22215"
                     /old_locus_tag="pgaptmp_004443"
                     /pseudo
     CDS             4413..4496
                     /locus_tag="ACHOSM_RS22215"
                     /old_locus_tag="pgaptmp_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4511..5164
                     /locus_tag="ACHOSM_RS22220"
                     /old_locus_tag="pgaptmp_004444"
     CDS             4511..5164
                     /locus_tag="ACHOSM_RS22220"
                     /old_locus_tag="pgaptmp_004444"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU4560805.1"
                     /translation="MNNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHITFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMVA"
     gene            5161..5550
                     /locus_tag="ACHOSM_RS22225"
                     /old_locus_tag="pgaptmp_004445"
     CDS             5161..5550
                     /locus_tag="ACHOSM_RS22225"
                     /old_locus_tag="pgaptmp_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4560806.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISEAGRKFQSAACAAI
                     IEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKTAGAAA"
     gene            5570..6379
                     /locus_tag="ACHOSM_RS22230"
                     /old_locus_tag="pgaptmp_004446"
     CDS             5570..6379
                     /locus_tag="ACHOSM_RS22230"
                     /old_locus_tag="pgaptmp_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGU4560807.1"
                     /translation="MNNLMVIDGIEVRRDVYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            6387..7376
                     /locus_tag="ACHOSM_RS22235"
                     /old_locus_tag="pgaptmp_004447"
     CDS             6387..7376
                     /locus_tag="ACHOSM_RS22235"
                     /old_locus_tag="pgaptmp_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4560808.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADVLPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MATKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            7394..>7751
                     /locus_tag="ACHOSM_RS22240"
                     /old_locus_tag="pgaptmp_004448"
     CDS             7394..>7751
                     /locus_tag="ACHOSM_RS22240"
                     /old_locus_tag="pgaptmp_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4560809.1"
                     /translation="MRDIQMVLERWGAWVANNHEDVEWSSVAAGFKGLIPSKVKSRPQ
                     CSDDDGLIISSAMTVLKKKEPYQYELLEMYYVYGVTLRALGVKLGISLNQVVIRLQKA
                     EGFIDGCLAMLGVSLEI"
BASE COUNT         1926 a         1810 c         2069 g         1946 t
ORIGIN      
        1 ctgcacatca gtactgcgtt cgctagccat tttgctgctc ctcatcatat cgaccctgca
       61 aggtcggttg gtttctccac aaaacagaga agaacacctg cggtgactgc cgcccggatg
      121 gattgggtta tgagcccgtc gtccggtgat gctcttctct gttttgtaaa aagagcggta
      181 ccagccggaa gcaagtgtac aaactggtac cgccaaagca gtggctgttg tggtgaccgg
      241 tgctgatctc cggcttgcgg ttatttcaga ctctcacggg cgtttaattg ccccgccgaa
      301 cagctctttt ccgcaatagc tgcaatgtct ttcgcgcatc agcctgcgca ttcaccacaa
      361 ctctaaaaac aaatgtagga tatccaacat gtaagtgtca agagtttatg ttggttatcc
      421 tacataaaaa gataggctca taaaaaaacc ggggataccc cggttttgcg atagtgagga
      481 agatgtgtca aaaatccatt attacttgtt tgacaagacc aactattctg cagttttcac
      541 cgcattcaat agttttatag ttaggattta gtgggacgag atacctgttc ggccagtcct
      601 caacaaattt tttgagtgtc gcttcttgcc caccattgat atgggcaaca acgatttttc
      661 cgttaataca ctctgtatca ataatatctg gctctacgat aacgatagaa ccttctggta
      721 tcgatggtga gccgagggga ttggtcattg aatcaccacg gacccgtagt gcaaatgcca
      781 tttctgatac aagggcggta gtataaaccc actcttcagc atcttctttc ctgacaccag
      841 gctccgtcat tgtccatgaa cccgcctgaa cccacgagat gagggggact tttttaactg
      901 cgaatatttc aggttttaga tttatctttg gttcaggcga gccttttccg ctaacaagcc
      961 acagaggatc gcatttaagt gcgttggcta gggcttgaag gttggctcca tttggttggt
     1021 agtcgtcctt ttcccatcca gtaaccgtga cacggttcac accagtcaaa tcagccagtg
     1081 cttgttgtgt caggttcagt tcttttcgcc tttggcgaat acgatcactc atgttcatca
     1141 tgtaggcaat cctaccacat gcccatgtag gattcttgac atttgcatgt tggatatcct
     1201 acatttctgc ttaacgtaat ttaacgggag acagaaatgc ggaaatccga cgtgattaat
     1261 tatttcggcg gagtttgtaa aaccgccgaa gccctaggta ttaagcatcc gtctgtttca
     1321 gagtggcctg agattattcc tgaaggccga gcgtaccagt tagaaaaaat tactaacggg
     1381 aaactgaaag ttgacgtgtc tttatatcaa aagactaaca gtgctgcggc ataaaaacac
     1441 cacagaaatg aggaattaac cgtgggtaaa gaacctgaat ggaaagttga taaacaacca
     1501 gcatggctgg tggcagcaat acgaagaacg attgctgatt tacctcatgg ctatgaggaa
     1561 gcagcagaaa ttcttggttt gtataaatct gatgatatca ccccagcaaa agatcaattg
     1621 cataacagac tgcgtagcgg tggggatcaa atttttccac ttgagtgggc catggtttta
     1681 caggatgcca gtggtaccag gcatgtaaca gatgcaatag cccgtcgtag taatggggtg
     1741 tttgtgccgc tggtggtcat tgatgacatt gacaatggtg acattaatca gcggctgatg
     1801 gagtcaatag aatggattgg caagcattcc cagtacttac gcaaggcaac tgctgatgga
     1861 gttattgacc aggctgagcg tgagcaaatc gaagagaaca gctaccaagt aatggcgaag
     1921 tggcaggagc atttaacact gttatttcgt gttttttgtg caccggaaaa gagtaacgcc
     1981 cgcgagtgtg cagctccggg cgtcgtggcg tcgattgctt ctggttgtgg agaaactaac
     2041 gcatgaacag tttaacggca aataaccgtt tgtcgcaaca gctggtggtc agcgtcgctg
     2101 aacacctgtt gttacggtat gaatgcagat taccaaatca cctggctgta agtaaccaca
     2161 gagaacttta cctgactgtg gggggcgagt tgtgcaggaa cttaaccgct ggtttcgtga
     2221 cggaagagga ctttatgttc atgttattcg ttgggagcca gaaacacagc gcgttatcta
     2281 tcttcgcaaa gactacccgc atgagtgctt tagtcctttg tggaaattca ggcgtgattt
     2341 tgttgagtgt gaaggaccac cagcacattg attctgccat tccgggacgt tacactgttc
     2401 aggcacctta taaagcgggt gccgggattg gcgtcctgaa attgattact gagcataacc
     2461 gcgctcatgc ggttttttcg tgtcatgagc attgctacgc ccaaattatg gtggggcgta
     2521 cagggccgac ttcggtcggg ccgggttcgg tagtctccgg taacgccaac cctgtacgtc
     2581 tcaccacctc tgtgattggc gtcccatgtg gtgagttttc tgaaaaactg actaccgggg
     2641 ctgtcaccat gactactctc ccaaccctct ctcaacctga aattgccatc gttgatggtc
     2701 aggctgttac atcttctttg gctgttgctg acttcttctc taaacgtcat gacgatgttc
     2761 tgaaaaagat ccgcattttg gattgttctc cagagttttg tgcccgcaat tttgcggaga
     2821 catcaatttt ggtacaccag cccaacggcg gtactcgcaa acttccttgc taccacatca
     2881 cccgcgacgg cttcgcgttc ctggcaatgg gcttcactgg caaacgtgct gcccagttca
     2941 aagaggcata catcaatgcc tttaaccaga tggagaaaca gctttcaaag ccctctgtac
     3001 cgagcgacgt tgcacataac gccagcgttc tctattccta cgtttcatca attcatcagg
     3061 tctggttgca gcagctttat cccatgctgg aaaaagctga atcaccgctg gctgtaagtc
     3121 tgtatgaccg aattaacgat gcggcatttc ttgcccgtct tattcattcg tcgctgaact
     3181 cttcagaggt aagggggcgc aaatgatccg gaatattttc aaacgattta ccaatcagac
     3241 tttccgttgt cctcgccccg gtcagtggta caccacgcct gcagggcatg ttctacgtgt
     3301 tagcctggtt gaccgtgaat gtcagaaggt gatttgtgaa ccgctgggcc gtaattaccg
     3361 cgtcagtatg ccgcttatag cctttcgctc cggaaaaaac atgaagcatc tcggaggtgc
     3421 ggcatgagcc tgttaatgac atcccagccc attgtgataa atagtgatct tgcatgtcgt
     3481 attggtctga atgaggcaat tgtgttgcag cagcttcatt actggctgaa tgaaacgaat
     3541 tcaggcactg agcatggcgg aattcgctgg gtttataaca cgacagaaca gtggctggag
     3601 cagtttccgt tctggtcaga gtccactctg aaacgcacat ttgcaagcct gaaatcactt
     3661 ggggttttgc gtcgcgagca actcaataaa tcgaagcgtg acatgaccaa cttctacacg
     3721 atcaactatg aaagtgagct tttagaagag gtcaaagtga acgaatcaat caggtcaaaa
     3781 tgcacttctc catcgggtca aagtgacctg atggatgggc gcaaaatgac acgatccatt
     3841 ggttcaaaac gacacgctgt catcgggtca aaatggccca atgatcttac agagaataca
     3901 acagagatta ctacagagaa taaaacctct tctcgtccgg acgcttcgca accggacacg
     3961 caaacggctg aacaggagtt tttaactcgc catcctgatg cggttgtatt cagccctaaa
     4021 aagcgccagt ggggaacgca ggatgatttg acctgcgcac agtggctctg gaaaaaaatc
     4081 atcgccctgt acgagcaggc cgccgaatgt gacggcgagg tggttcgtcc caaagaaccg
     4141 aactggacag cctgggcaaa cgaaattcgc ctgatgtgtg tgcaggatgg tcgtacccac
     4201 aaacaaatct gcgagatgta cagccgcgtc agccgcgatc cgttctggtg ccgtaacgtg
     4261 ctcagcccgt cgaagttgcg ggaaaaatgg gatgagcttt ccctgcgctt atcgccgtcc
     4321 gtcagcacgc acacagaaaa acgtgaagac ccgtacttca aagccagtta cgacaacgtg
     4381 gactacagcc agatcccggc aggattcagg gggtgatcat gagtctgtta aatgacgttc
     4441 agaaattcat tgaagcccat ccgggctgta cttccggaga cattgcggat gctttttacg
     4501 tgggggctta atgaataata aatattgcca ggcgctggta gaactgcgga acaaaccagc
     4561 ccatgaactg aaggaagtgg gcgatcagtg gcgcacgccg gacaacattt tctggggaat
     4621 taacaccctg tttggcccgt ttgttctgga tctgtttact gacggtgata acgccaaatg
     4681 tgccgcgtat tacacggcgg aagataacgc gctggcgcat gactggtcag aacgtcttgc
     4741 ggagcttaaa ggtgctgcct ttggtaatcc cccatacagc cgcgccagtc agcatgaggg
     4801 gcaatacatc accggcatgc gttacatcat gaaacatgcc agtgccatgc gtgataaggg
     4861 tgggcgctat gttttcctga tcaaagctgc caccagcgaa gtgtggtggc cggaagatgc
     4921 ggaccatatt acttttattc gcgggcgtat tggttttgaa ctgcctgcct ggtttatccc
     4981 gaaggatgag aagcaggtgc cgacaggcgc tttcttcgct ggtgctattg ctgttttcga
     5041 taagacctgg aagggaccgg caatcagcta catcgggcgc gatgaacttg aggcatgtgg
     5101 tgaggcgttt ctggcgcagg ttcgccagca ggcggaaaaa ctggtcaggg agatggtggc
     5161 atgaagctaa tactgccttt tccgcccagc gtgaacacgt actggcgaca ccccaacaaa
     5221 ggggcatttg ctggtaagag cctgataagc gaggcggggc gaaaatttca gagcgcggcg
     5281 tgtgccgcca tcattgagca gttacgtcgt ctgccgaaac caacgtcggc acctgcttca
     5341 gtggagatcg tgttgtttcc tccggataac aggatccgcg atctggacaa ctataacaag
     5401 gcgctgtttg acgccctgac ccacgcgggt gtgtgggaag acgacagtca ggtgaaaaga
     5461 atgctggtgg agtggggacc ggttatcccg aaagggaagg tcgagatcac tatcagtaag
     5521 tacgagaaaa cggcgggtgc agccgcctga tcaagaggag aaacgaagta tgaataatct
     5581 gatggtcatt gatggtattg aagttcgtcg tgatgtttat gggcgttaca gcctgaacga
     5641 tctacatcgc gcagcagtag catctggtgc aaatgccaga accaaggagc caggaaagtt
     5701 tctttccagc caacaaactg ttgagcttgt tcatgaattg accaacaccc agaatttggg
     5761 tgttgacccg gtgagtgtga ttcatggggg aaatgaacgg ggaacgtatg tctgcaagga
     5821 actggtgtat gcctatgcaa tgtggatcag cccgtcattc catctgaagg tgatccgtac
     5881 tttcgacatg gtaaccagcg caccggaaaa attatccgga caggctgctg acaagatgca
     5941 ggctggtgtg attctgctgg actttatgcg ccgggaatta aacctgtcta actcttcagt
     6001 gcttggtgcc tgtcagaaac tccaggaggc tgttggctta ccgaatctgg caccgcgcta
     6061 tgccattgat gctcctgctg acgcgcctga tggctcaagc cgccccacgc tgtcactgag
     6121 tgcactgctg aagcagtatg gtatccgcct gacagctaat caggcatatc accagatggc
     6181 gaagctgggg atcgttgaac aacgcgaacg atacagtcgt accgcgatta acaacatcaa
     6241 aaaattctgg tcgctgaccg cgaaaggctg catgttcggc aagaacatca ccagtcctgc
     6301 aaatccgcgc gagacgcagc cgcatttctt cgaatcccga ttccctgagc tgttaaagct
     6361 gctcgatacc gttcattgag gtgaccgtga gagcactact gacccctgaa attgccccgc
     6421 gtatggggat cgtattgttc agaccaggtt cagagctgat gcccctgttt atgcaggggc
     6481 gtgtcctgct ggagcctgag ccggaacgtt attcatcttt cgccagtggt gccgtaccgg
     6541 cggcatcaca accgctggcg gatgatcctg ccgttcgggc cgtgttccgc aatgaggcag
     6601 tgatccgtcg tgctggtggc gtggaatgtc ttgaaagctg gttacttcgt gaaaaaggct
     6661 gccagtggcc tcattccgac tggcacagcg agaacatgac aacaatgcgg cacgcgccgg
     6721 gcgcaatccg tctgtgctgg cactgcgata accagctgcg cgatcagttc acggaacgac
     6781 tggaatcaat ggcaacggat aactgtgccc gctgggtgtt gtctgttgtg cgtcgggatc
     6841 tcggttttga tgatagtcac gttgtgacaa tgccggaact gtgctggtgg ctgattcgta
     6901 atgacctggc ggatgtctta ccggaaagtg cagcccgtaa ggcactgaga ttaccaaagc
     6961 ctgttgtgcc gtctgtcacc cgggaaagtg accttgtgcc ttcggttcct gccaccagca
     7021 tcatccagga taaggcaaaa aaggtgctgg cgctgaaagt ggatccggag tcgccggagt
     7081 cttttatgtt acgcccaaaa cgtcgccgct gggttaatga aaagtacacg cgctgggtta
     7141 agacgcagcc gtgcgcatgt tgtggaaagc ctgctgatga tccccaccac ctgataggtc
     7201 acggtcaggg tggaatggct acaaaagcgc atgacctctt tgtgttgcct ttgtgcagaa
     7261 agcatcacga cgagctgcat gcggataccg tggcatttga agagaagtat ggctcccagc
     7321 tggagctgat atttcgtttt atcgatcgtg cgctggcaat aggcgtgctg gcctgatttt
     7381 gtggagaaag ttgatgcgtg atattcaaat ggttcttgaa cgttgggggg catgggtggc
     7441 aaataatcac gaggatgtgg aatggtcatc tgttgctgca ggttttaagg gattaattcc
     7501 ttcgaaagta aaatcccgcc cgcaatgtag cgatgacgat ggcctgatca ttagctctgc
     7561 gatgacagtt cttaagaaaa aggaaccgta tcaatacgaa ttactggaaa tgtattatgt
     7621 gtatggggtt acattacggg cgttgggggt aaaactgggg atatcactta atcaggttgt
     7681 tatcagactg cagaaagctg aagggtttat tgacggttgt ctggcaatgt tgggggtatc
     7741 tttagaaatt g
//
LOCUS       DAZKHC020000163         7695 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZKHC020000163 DAZKHC020000000
VERSION     DAZKHC020000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.163"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..823
                     /gene="gadX"
                     /locus_tag="ACHOSM_RS22245"
                     /old_locus_tag="pgaptmp_004449"
     CDS             <1..823
                     /gene="gadX"
                     /locus_tag="ACHOSM_RS22245"
                     /old_locus_tag="pgaptmp_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HGU4560810.1"
                     /translation="QSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVD
                     KCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQN
                     KNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTL
                     ARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYH
                     SVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1191..1919
                     /gene="gadW"
                     /locus_tag="ACHOSM_RS22250"
                     /old_locus_tag="pgaptmp_004450"
     CDS             1191..1919
                     /gene="gadW"
                     /locus_tag="ACHOSM_RS22250"
                     /old_locus_tag="pgaptmp_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HGU4560811.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2282..5395)
                     /gene="mdtF"
                     /locus_tag="ACHOSM_RS22255"
                     /old_locus_tag="pgaptmp_004451"
     CDS             complement(2282..5395)
                     /gene="mdtF"
                     /locus_tag="ACHOSM_RS22255"
                     /old_locus_tag="pgaptmp_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HGU4560812.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFIPVFFVVVEHL
                     FARFKKA"
     gene            complement(5450..6529)
                     /gene="mdtE"
                     /locus_tag="ACHOSM_RS22260"
                     /old_locus_tag="pgaptmp_004452"
     CDS             complement(5450..6529)
                     /gene="mdtE"
                     /locus_tag="ACHOSM_RS22260"
                     /old_locus_tag="pgaptmp_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HGU4560813.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSS"
     gene            6637..6915
                     /locus_tag="ACHOSM_RS22265"
                     /old_locus_tag="pgaptmp_004453"
     CDS             6637..6915
                     /locus_tag="ACHOSM_RS22265"
                     /old_locus_tag="pgaptmp_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560814.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKIVLASPLVITGGNEISICIPRHASHQFQLFMS"
     gene            complement(6916..7443)
                     /gene="gadE"
                     /locus_tag="ACHOSM_RS22270"
                     /old_locus_tag="pgaptmp_004454"
     CDS             complement(6916..7443)
                     /gene="gadE"
                     /locus_tag="ACHOSM_RS22270"
                     /old_locus_tag="pgaptmp_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HGU4560815.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLETT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         1983 a         1807 c         1839 g         2066 t
ORIGIN      
        1 gcaatcatta catgggaatt gtctaattgc gtacgcaaga cataaatata ttctcaccat
       61 ggttaatggt gaatatcgct attttaatgg cggtgacctg gtttttgcgg atgcaagcca
      121 aattcgagta gataagtgtg ttgaaaattt tgtattagta tcaagggata cgctttcatt
      181 atttctgccg atgctcaagg aggaggcatt aaatcttcat gcacataaaa aaatttcttc
      241 attactcgtt catcactgta gcagagatat tcctgttttt caggaagttg cgcaactatc
      301 gcagaataag aatcttcgct atgcagaaat gctacgtaaa agagcattaa tctttgcgtt
      361 gttatctgtt tttcttgagg atgagcactt tataccgctg cttttgaacg ttttgcaacc
      421 gaacatgcga acacgagttt gtacggttat caataataat atagcccatg agtggacact
      481 agcccgaatc gccagcgagc tgttgatgag tccaagcctg ttaaagaaaa aattgcgcga
      541 agaagagaca tcatattcac agttgcttac tgagtgtaga atgcaacgtg ctttgcaact
      601 tattgttata catggctttt caattaagcg agtcgcagtg tcctgtggat atcacagcgt
      661 gtcgtatttc atttacgtct ttcgaaatta ttatgggatg acgcccacag agtatcagga
      721 gcgatcggcg cagggattgc cgaaccgtga ctcggcggca agtattgttg cgcaaggaaa
      781 tttttacggc actgaccgtt ctgcggaagg aataagatta tagaatttta ctcagacata
      841 aaaaaaaccc ggcatagggg accgggaaga ggatagtctg ccgtctccag actaataaac
      901 cgttataaca ctccctgttg gcacgggaaa ctttgtgctc tcagtaagtt aaatataact
      961 tttactggaa ataagatcag ccattttttt ataaacataa gctatacgct gtgcgaaaat
     1021 atattcgtgc tgcatttact tattatcaat taactgttat gcaaaactac tttgtggata
     1081 aattttggtc ctaccaaatc tggcagtttt tgcgctaaga aacagtctgg caacatttca
     1141 ttagtatact gaaattgaaa taatcgcagt atgaaatata agggataatc atgactcatg
     1201 tctgctcggt gatcctcatt cgtcgttcat tcgatattta tcatgaacag cataaaatat
     1261 cgctgcataa cgagagtatc gtgctgctgg agaaaaattt ggcagacgat tttgcgtttt
     1321 gttcaccgga tacgcgacga ctggatatcg atgagctgac agtttgccat tacttacaaa
     1381 atattcgtca gctaccacgc aatttagggt tacatagcaa agaccgtcta ttaattaacc
     1441 agtcaccccc catgccgctg gtgacggcga tttttgatag cttcaatgaa tccggggtaa
     1501 attcaccgat actgagcaat atgctctacc tttcctgttt atcgatgttt tctcataaga
     1561 aagaactgat ccccttactt ttcaatagca tcagcactgt ttcaggaaaa gttgaacgcc
     1621 ttattagctt tgatatcgcc aaacgttggt atctgcgcga tatcgcggaa agaatgtata
     1681 ccagcgagag tctcatcaaa aaaaagttgc aggatgaaaa tacctgtttc agtaaaatat
     1741 tactcgcttc caggatgtcg atggccagac gattactcga gttacgtcaa attcctctgc
     1801 atactattgc agaaaaatgt ggctatagca gtacgtcgta ctttataaac acatttcgac
     1861 aatattatgg tgtaacgcca catcagtttg cgcaacattc gccaggtacc ttttcctgac
     1921 atattttgca tttgaatatt ggtcaggatc tcacacctgc ttcatgtgaa actcttccct
     1981 gatgatttct gccgggctac cggctagttc tctttcgcag tttgccaggc ggtataggta
     2041 ttttcaggag gaaaagactc ggcatgtttg ggattattaa gctgacaatt catacgatta
     2101 cgggaatgtg ggtcagtatt gtgctgttca aattgatgac gaatggctgg agtggtttct
     2161 actttcagtg ctgtgtacta agcctggtat tcctgacggt tagctggttg ttgagtggtg
     2221 aatggttagc aggaaagagt aaggctgaac cttcacgttc aaccttactc tcatttacac
     2281 gttacgcttt tttaaagcgg gcaaagagat gttccactac aacgaaaaag accggaatga
     2341 agtaaattgc cagcactgtt gcggcaaaca tcccgcccat cacgccggta cctaccgcgt
     2401 tttgcgcgcc agaaccggca ccatgactga taaccagcgg cagcacgccg agaataaagg
     2461 ccagagaggt catcaggatt gggcgtaaac gcatccgcgc cgcttcgatg attgcctcta
     2521 tcggcgtttt cccttctttc tgcatcatct caacggcaaa ttcgacaatc aggatggcgt
     2581 tcttggcgga aagcccgatg gtggtcagca aaccaacctg gaagtagacg tcattactta
     2641 agccgcgcag atcggtggcc agtaatgcgc caacgacgcc taacggaaca accaacatca
     2701 ccgagaacgg aattgaccag ctctcataga gtgcggcgag ggcgaggaac accacgacca
     2761 gtgaaatggc atacagtgca ggagcctggt ttgaggataa cgcttcctga tacgacagtc
     2821 cggtccatga gtagccgacg cctgccggaa gtttagcgac caggtctgcc ataaatttca
     2881 tagcgtcacc ggtacttttc ccggccgccg cttcacctaa aatctccatt gacgggatgc
     2941 cgttgtagcg ttccaatcgc ggtgaaccat aggtccattc ggtagacgag taggcagaaa
     3001 gcggtgccat cgtgccagag gcgttgcgta cataccattg gttgatgtta tccggcaaca
     3061 tacggaacgg cgtgcctgcc tggacataca cttttttcac ccgcccctgg ttgaggaagt
     3121 cgttcacgta gctgctgccg aaggcggtgg aaattgtctg gttgatatca gacagcgcca
     3181 cacccattgc ctcagctttc gcggcgttga cgttcacttt gaacatcggc gtatcttcca
     3241 ggccgttcgg gcgtaccccg gtgacctggt caggcgattg cgctgccagt gataacagct
     3301 cgtttcgtgc ctgggttagt ttttcgtgcc ccaggttacc gttgtccagc agttccatat
     3361 caaaacctga cgcggtaccc agttcagcca ccgcgggtaa gttgaacggg aagacgacgg
     3421 ctttattgat actgcttaac gcaatcattg cccgctgaat gatcgcggta accgagtttt
     3481 cctcaccgac acgttcagac cacggcttga gactgataaa cgccaggccg ttgttttgcc
     3541 cctggccgct gaagccaaag ccgccaacgg taaacaccga ctgaacatta tctttctctt
     3601 ttgtaagata ataatccgtc acctgttgca gcactttcgt ggtgttaacc atggtggcac
     3661 cggaaggtaa ctgcgcggtg gtcataaata ccccctgatc ctcttctggt aagaaagagg
     3721 tcggtgtacg caggaacagc accgccatcc cggcgcaaat cagcaggtag accaccatgt
     3781 agcgaccggt acaacgcaac agcgagcggg tgctatcggt atagtgctga gttgattttt
     3841 caaacagcgt gttgaagcgt gcgaacaggg cgttaggttt gtgaccgcct tccggcgcgg
     3901 ctttcagaat ggtggcgcac agggcagggg tcaggctcat tgccacaaat actgaaagca
     3961 gcatggagga gatcagcgtg atggagaact ggcggtagat ctccccggtt gcgccgctca
     4021 taaaggccat cggcataaac actgcggaaa gaacaacagc aataccgacc agcgcacgtt
     4081 ggatctgccc catcgattta tgcgtcgctt ccttcggtgg tagcttatct tccgcaatga
     4141 cacgctcgac gttctccacc accacgatgg cgtcatccac cagtaaccct atcgccagca
     4201 ccatcccgaa catagttaac gtgttgatgg tgaaaccgac cgccgacaag atcgcaaacg
     4261 tcccgagaat aaccaccggt acggcaatcg tcgggatgat tgtggcacgg aaattctgca
     4321 aaaacagata catgaccagg aagacgagga tgatagcctc aaccagtgtt ttaaaaactt
     4381 cctgaataga aatttcgata aacggcgtgg tgtcgtaagg ataaaccgtc ttcagacttg
     4441 cggggaaata ggctgataag cggttcagct cctctttgac tgcccgcgag gtatccaggg
     4501 cgtttgctcc ggtagccagt ttgatggcga tcccggcagc aggtttgccg ttatagcgcg
     4561 ccacggtgga ataatcttcc gctccaagtt cgacgcgggc gacatcacgc agcagcactt
     4621 gcgaaccatc ttgctgaact ttcaacagga ttttgccaaa ttcttccggc gtttgcagac
     4681 gcgtctgcac aatgatcgag gcgtttagtt gctggtctgc cgcctgtggc atgccaccca
     4741 gttgaccacc ggaaatctgg ttgttttgca ccttaatctg ggaaataaca tcggaaggta
     4801 ccagattgta tttattgagt ttttgcgggt ccagccagat acgcatggcg tactcggaac
     4861 caaagagctg tacgctacca acgcccgcgg tacggcttag cgggtctttg atattagacg
     4921 ctacatagtc cgcgatatcg tactggttga ggctgccgtt atcagaaata aacgccgcta
     4981 ccatcagaat attactgctc gacttatcaa cgctaatccc ctgctgctgc accgcttcag
     5041 gtaatgaagg catagcgagt tgcagtttat tttgcacctg aacctgtgcg atatcaggag
     5101 atgtcccagt ctcgaaggtc agagtgatag aggcattacc cgccgcatca ctggttgaag
     5161 acatgtacat caggccatca agcccattca tattttgctc aatcacctga gtgaccgagt
     5221 cttctaccgt ttgcgcatcg gcacctggat aggtagcgct gacggtaatg gtcggtggcg
     5281 caatctgcgg atactgcgca accggtaagt tcatgatcgc cagaccacct gcaagcatca
     5341 taataatggc aagtacccag gcaaaaaccg ggcgatcaat aaaatagtta gccatgaaag
     5401 tccccttaag cctgcaacgt tattgtttcg attcggtgct ggcgttttcc tagctggagg
     5461 aaattgctcg tgctttgata cccggacgaa tgcgttgcaa accggaaacg atcacccgat
     5521 cgccagcctg caagccagag gtgacaaccc actggtcgcc gatggctttg ctggcttcaa
     5581 tttcgcgtag ctgcacaaca tcgtctttat ccagaatgag cgccgttgct ttaccctggg
     5641 cgttgtgggt gacgccttcc tgcggcacca gtaatacatt ctggcggcta ccttcatcca
     5701 ctaatgccgt gacgtacatg ccaggcagca agtcaccatt tgggttgggg aaaatcgccc
     5761 gtaacgtcac ggagcccgtg gtttcatcca ccgtcgggtc ggagaatttc agcgtgccgg
     5821 tctggctgta gcgtttacca ttttccagat tgagctgtac tggcgtactg ccctgaacct
     5881 gtttgatttg cccactggcg acctcttctt tcatgcgtaa gaaatcttgc accgactgcg
     5941 tgagatcgac ataaatcggg tccagacgtt gtacggtaac cagcgaatct gcctgattag
     6001 cggtaacgag tgcgccgacg gtcaccgacg atttcccgct gacgcccgta atcggcgagg
     6061 tgacattcgc gtattgcaga ttgatcgtcg cctgttcaac agccgctttg gcgacggtga
     6121 cattggcttc tgcttcattc aactgggtgc gcgcggtgtc gtaatcctga cggctaacgt
     6181 agttggtctt cagcaacgat gcctggcggt taaaggtgat gcgggcattg ctggcggtag
     6241 agagcgcttt cgccagcgag cctttggcgg agtttagctc ggcctgtaaa ggtgcaggat
     6301 caatctgata cagcgaatcg ccctggttca ctttatcgcc ttcgataaag ttgcgtttaa
     6361 tgataatacc gcccacctgg ggacgtatct cggcaacttc ataaggaacg gttctaccgg
     6421 gcaattcgct caacacattg accgaaccgg gggagagtgt gacgacaccg acctcaggcg
     6481 tcatggcggc ggcgttttcc gccgatttgt catcgcaggc ggtgagcatc gcgccgcaga
     6541 ataacaacgg tattaacagc tttcttcttc tgttcatttt agtccctgaa aattcttgag
     6601 aaataacggt tctttaaatt gcgcgcgagg agagatatga gggatttaca gaccagcggc
     6661 atagtggggt tgtcagccag taaggtggga tacaggcaca gtgatcgaca tggtgaggtc
     6721 aacgacaaaa aaaatgttgc cgttctgcca acagttcctg ccagcattcg ggcaacgaag
     6781 ggttctactg gtggatacac ataccagggg aataaaatcg ttttagcctc tcctttagta
     6841 atcaccgggg gtaatgaaat cagcatctgc atccctcgtc atgccagcca tcaatttcag
     6901 ttgtttatgt cctgactaaa aataagatgt gatacccagg gtgacgatgt cgctcatacg
     6961 tcttagtttc agtttgtaca taatgttgaa tttctgaatc tttaatgttt gttgcgaaat
     7021 attgagcgtc gacgtgatat tgctttcatt tttatttttc agaatcagtt gcataatttt
     7081 gaactgatta tgtgaaaaac ataattgtga attcttatgg ggcaagtgtt taccataagt
     7141 agtctcaaga agatttttga tactttcttt gcggctaaca aaataaatag gcgctttagc
     7201 ttttagttta ctgatgtgat aaggtgccaa aacaataatt ctctcggcat ctaatttctc
     7261 cagaaattta atcgcttctt catcaaggat atgattgtga taggtatcaa tgataacctt
     7321 gaagggttta ttgcctactt ttttgatctc tgacagggaa ttgattttta tcatttcgtg
     7381 attatctttc aactgccaaa agccctgtaa aagaaaagaa tctttcgtca tgagaaaaat
     7441 cataacttgc tccttagccg ttatcgtttc gaacatgtca tccttttcat tatttacatc
     7501 cttgtccgaa tcgttgttca atatagtaaa cgcctataag aactttgccc ttcgtgaact
     7561 aatacgtcta ttaacactaa tcaatacttc agatttatcc atcacgcact ccatatattg
     7621 atcctaagta agattcctgg ttgttatcaa tttgtaaata tcaaacaaaa agttaacgtt
     7681 tgcggattac tgccc
//
LOCUS       DAZKHC020000164         7667 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZKHC020000164 DAZKHC020000000
VERSION     DAZKHC020000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.164"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            7..1731
                     /gene="iucA"
                     /locus_tag="ACHOSM_RS22275"
                     /old_locus_tag="pgaptmp_004455"
                     /pseudo
     CDS             7..1731
                     /gene="iucA"
                     /locus_tag="ACHOSM_RS22275"
                     /old_locus_tag="pgaptmp_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
     gene            1732..2679
                     /gene="iucB"
                     /locus_tag="ACHOSM_RS22280"
                     /old_locus_tag="pgaptmp_004456"
     CDS             1732..2679
                     /gene="iucB"
                     /locus_tag="ACHOSM_RS22280"
                     /old_locus_tag="pgaptmp_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HGU4560816.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWCEEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     VNRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            2679..4421
                     /gene="iucC"
                     /locus_tag="ACHOSM_RS22285"
                     /old_locus_tag="pgaptmp_004457"
     CDS             2679..4421
                     /gene="iucC"
                     /locus_tag="ACHOSM_RS22285"
                     /old_locus_tag="pgaptmp_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HGU4560817.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDQDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            4418..5754
                     /gene="iucD"
                     /locus_tag="ACHOSM_RS22290"
                     /old_locus_tag="pgaptmp_004458"
                     /pseudo
     CDS             4418..5754
                     /gene="iucD"
                     /locus_tag="ACHOSM_RS22290"
                     /old_locus_tag="pgaptmp_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
     gene            5760..>7667
                     /gene="iutA"
                     /locus_tag="ACHOSM_RS22295"
                     /old_locus_tag="pgaptmp_004459"
     CDS             5760..>7667
                     /gene="iutA"
                     /locus_tag="ACHOSM_RS22295"
                     /old_locus_tag="pgaptmp_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HGU4560818.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLRQELVGQVYYRDESLRFYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYISW"
BASE COUNT         1797 a         1976 c         2079 g         1815 t
ORIGIN      
        1 ttgactatga ccctgccctc tgaaaaacca gccacagatg tggctgcgca gtgcttcctg
       61 aatgcactga ttcgtgaaac tacagactgg aaactgacag aatacccgcc agacgaattg
      121 atcatcccgc tggatgagca gaaatcgctc catttcagag tggcttattt ctccccaacc
      181 cagcatcacc gctttgcatt tcctgcacgt ctggtcacgg catcaggcag ttatcctgtc
      241 gactttacca ctctctcccg gcttattatt gataagctac ggcatcaact gtttctgccc
      301 gttcccctct gcgaaacttt ccaccagcgc gtgctggaaa gccacgccca tacgtaacag
      361 gcaattgatg cccgtcatga ctggagcgcc ctgcgtgaaa aagcgttgaa ttttggcgaa
      421 gctgagcagg cgctgctgac aggacacgct ttccaccctg cgcctaagtc tcatgaaccg
      481 tttaaccggc gggaggctga acgctacctg cctgatatgg caccccactt cccactgcgg
      541 tggttttcgg tggataaaac gcaaatcgcc ggtgaaagtt tgcatctcaa ccttcaacag
      601 cggctgacgc gatttgccgc agagaatgcg cctcagttac tcaacgaatt aagtgacaac
      661 caatggctgt tcccgttgca tccgtggcag ggggaatatc ttttgcagca gggggggtgc
      721 caggcacttg ttgctaaagg gctgattaaa gacttaggtg aggccggcac gtcgtggctg
      781 ccgaccacct cttcccgttc cctctactgt gccaccagcc gcgatatgat caagttctcc
      841 ctgagcgtac ggctgaccaa ctccatccgt actctgtccg tgaaagaagt gaagcgtggg
      901 atgcgcctgg cacgcctggc tcaaaccgac ggctggcaga tgctacaggt ccgcttcccg
      961 actttccggg taatgcagga ggatggctgg gccgggctgc tcgatcttaa cggcaacatc
     1021 atgcaggaaa gtctgtttgc cctgcgtgaa aatctgctgg tggatcagcc gaaaagccag
     1081 accaacgtac tggtctccct gactcaggcc gcacctgatg gcggtgattc gctgctggtt
     1141 tcggcggtaa aacgcctgag cgatcgcctc ggtatcactg tgcaacaggc cgcccatgca
     1201 tgggtcgatg catactgtca gcaagtgcta aagccgctgt ttacggctga agcggattac
     1261 ggcctggtgc tgctggcgca tcagcaaaat attcttgtcc agatgcttgg ggatctgccg
     1321 gtcggattta tttaccgtga ctgtcagggc agcgctttta tgcctcacgc gacagactgg
     1381 ctcgattcta ttggcgaggc gcaggcggaa aatattttca cccatgagca gttgctgcgc
     1441 tatttccctt attacctgct ggttaactcc acttttgctg tgaccgctgc gctgggggct
     1501 gccgggctgg acagcgaatc gaatctgatg gctcgtgtac gagcatcgct ggctgaagtg
     1561 cgtgatcagg tgactcataa aacctgtctc aactacgtgc tggaaagtcc gtactggaac
     1621 gtaaaaggta actttttctg ttatctgaac gatcataacg agaacaccat tgttgaccct
     1681 tcggtgatct atttcgattt cgctaacccg ctgcaggctc aggaggtctg aatgtctgag
     1741 gcaaacatta ttcacagcag atatggactg cgctgtgaaa aactcgacaa gcccctgaat
     1801 cttggctggg gactggacaa tagcgcggtg ttgcactgtc ccggggagct gccgacaggg
     1861 tggctgtgcg atgcgctgga tcagatattt atcgccgcac cacaactgtc agcagtggct
     1921 ctcccctggg ccgaatggtg tgaggagcca caggcgctga cgctttttgg gcaggtaaaa
     1981 agcgacatta tccatcgtac cgccttctgg cagttaccgt tatggttgag ttcaccggta
     2041 aaccgggcct ccggtgaaat ggtttttgat gcagagcgtg agatttattt cccgcagcgc
     2101 ccaccccgtc cgcagggtga ggtttaccgc cgttacgatc cacgtattcg caggatgctg
     2161 agtttccgca ttgccgatcc cgtttctgat gcagaacgtt tcacccgctg gatgaatgat
     2221 ccgcgcgttg agtatttctg ggagcaaagt ggctcactgg aggtacagac cgcctatctg
     2281 gagcgccagc tcaccggtaa acatgcattc ccgctgattg ggtgcttcga cgatcggcca
     2341 ttcagctatt ttgaaatcta ctgggcggcg gaagaccgca ttggtcgtca ctattcgtgg
     2401 cagccctttg accgtggcct gcatctgctg gttggtgaac agcaatggcg cggggcccac
     2461 tatgtgcaaa gctggctgcg cgggctgaca cattacctgc tgctggatga gccccgtacg
     2521 cagcgcacag tactggagcc acgcaccgat aaccagcgcc tgttccgcca tcttgagcct
     2581 gcgggatacc ggacaattaa agagttcgac ttcccacata agcgctcgcg catggtgatg
     2641 gcggatcgtc atcacttctt cacggaggtc ggtctgtaat gaatcacaag gactgggatt
     2701 ttgttaaccg tcggctggtg gcaaaaatgt tgtctgagat ggagtatgag caggttttcc
     2761 acgcggaatc tcagggcgat gaccactact gcattaacct gccgggagca caatggcgct
     2821 tcatcgctga acgtggtatc tggggctggc tgtggattga tgcccaaacg ttgcgctgca
     2881 cggacgaacc agtactggcg cagacgctgc tgatgcagct aaagccggtg ctgtcaatga
     2941 gtgatgccac tgttgcagag catatgcagg atttgtatgc cacgctactg ggcgacctgc
     3001 aactgctgaa ggcccgtcgc ggactgagtg ccagtgacct gattaatctt gatgccgatc
     3061 gtctgcaatg cctgctgagc ggtcatccta aattcgtttt taataaaggt cgccgtggct
     3121 ggggcaaaga ggcgctggaa cgatatgcgc cagagtatgc caacaccttc agactgcact
     3181 ggctggcggt aaaacgtgaa catatgatct ggcgctgcga caacgatctg gatattcagc
     3241 agttgttgac tgccgcaatg gatccgcagg agtttacacg cttcagtcag gtctggcagg
     3301 aaaacgggct ggatcacaac tggctgccgc tgccggtaca tccgtggcag tggcagcaaa
     3361 aaatcgccac cgacttcatc gctgattttg ccgaaggcag gatggtgtct ctcggcgagt
     3421 ttggcgacca gtggctcgcc cagcagtcgc tgcgtaccct gaccaacgcc agccgccggg
     3481 gagggctgga tatcaagctg ccgctgacca tctacaacac ctcatgctac cgggggatcc
     3541 ctggcagata tatcgctgcc ggaccactgg cttcacgctg gctgcaacag gtttttgcga
     3601 ccgacgccac cctggtgcaa agcggcgcag tgatccttgg tgaaccggct gcaggctatg
     3661 tgtcccatga aggctatgcc gcgcttgccc gggctcccta tcgctaccag gaaatgcttg
     3721 gtgttatctg gcgggagaat ccgtgccgct ggctgaaacc ggatgaaagt ccggttctga
     3781 tggcaacact gatggagtgc gacgaaaaca atcagccgct ggcaggcgca tatatcgacc
     3841 gctccggact ggacgctgaa acctggctta cgcaattgtt ccgagtggtt gtggttccgc
     3901 tgtatcacct gctttgccgc tacggtgtcg cgcttattgc acacggacaa aatataacgc
     3961 tcgccatgaa agagggggta ccacagcgtg ttctgctgaa agacttccag ggcgatatgc
     4021 ggctggtgaa agaagcgttt cccgaaatgg actctttgcc tcaggaggtg cgtgatgtca
     4081 catcccgtct gagtgcggac tacttaatcc atgatttgca gacgggtcac ttcgtgacag
     4141 tactgcgttt tatttcgcca ctgatggttc gtcttggcgt acctgaaagg cgattttatc
     4201 aactgctggc agcagtgttg agtgattaca tgaacaaaca tccacaaatg tcagagcgtt
     4261 ttgcgctttt ctcacttttc aggccacaaa tcattcgcgt ggtgctgaac ccggtaaaac
     4321 tgacctggcc ggaccaggat ggcggcagtc gcatgctgcc gaattacctt gagaatctgc
     4381 aaaatccgct gtggctggta actcaggaat atgaatcatg aaaaaaagtg tcgattttat
     4441 tggtgtcggg acagggccat ttaatctcag tattgctgca ttgtcacatc agatcgaaga
     4501 actgaactgt ctcttctttg acgaacatcc tcatttttcc tggcatccgg gtatgctggt
     4561 accggattgt catatgcaga ctgtctttct gaaagatctg gtcagtgcag ttgcacctac
     4621 aaatccctac agctttgtta actatctggt gaagcacaaa aagttctatc gcttccttac
     4681 aagcagacta cgtacagtat cccgtgaaga gttttctgac tatctccgct gggctgctga
     4741 agatatgaat aacctgtatt tcagtcatac cgttgaaaat attgatttcg acaaaaaagt
     4801 cgattgtttc tggttcagac cagtcgggga gaatattttg cccgcaatat ctgccttggt
     4861 acaggaaaac aaccttattt accaccctgt gtgaagcatg tgacacaatc ctgtttccat
     4921 gccagtgaaa tgaatcttcg tcggccggac ctgagtggaa aacggataac tgtggttggt
     4981 ggaggacaga gtggtgcaga cctgttcctt aatgcattac gcggggaatg gggagaagcg
     5041 gcggaaataa actgggtctc acggcgtaat aattttaacg cactggatga ggctgctttt
     5101 gctgatgagt attttacacc tgaatatatt tcaggcttct ccggactgaa ggaagatatt
     5161 cgccatcagt tactggatga gcagaaaatg acatcggatg gcatcactgc cgattcttta
     5221 ctgaccattt atcgtgagtt gtaccaccgt tttgaagttc tgagaaaacc aagaaatatc
     5281 cgtctgctac ccagccgctc ggtaacaact ctggaaagta gtggtccggg ctggaagtta
     5341 ttgatggaac atcatctgga tcggggcaga gagagcctgg aaagtgatgt ggtgattttc
     5401 gccacaggtt accgttctgc attgccacaa atacttccct cactgatgcc cttgatcacc
     5461 atgcacgata agaacacctt taaagtgcgt gatgacttca ctctggaatg gagtggcccg
     5521 aaagaaaata acatctttgc ggtcaacgcc agcatgcaaa ctcatggcat cgccgaaccc
     5581 cagctcagcc tgatggcctg gagatctgca cgtattctta atcgcgtact ggggcgtgat
     5641 ttattcgatc tcagtatgcc gcccgcactg attcagtggc gcagcggcag ccggaaaaaa
     5701 ccgcagccgg aggctgctgc tttaactcac tatacaacaa atattcagga ataacgatga
     5761 tgcgcaaaaa gtatatgccc cgggctcttg gtccgctgct tcttgtcgtg ctgtcaccag
     5821 ctgtcgccca gcaaaacgat gataatgaga tcatagtgtc tgccagccgc agcaatcgaa
     5881 ctgtagcgga gatggcgcaa accacctggg ttatcgaaaa tgccgaactg gagcagcaga
     5941 ttcagggcgg taaagagctg aaagacgcac tggctcagtt aatccccggc cttgatgtca
     6001 gcagccagag ccgaaccaac tacggtatga acatgcgtgg ccgcccgctg gttgtcctga
     6061 ttgacggtgt gcgcctcaac tcttcacgtt ccgacagccg acaactggac tctgtcgatc
     6121 cttttaatat cgaccatatt gaagtgatct ccggcgcgac ggccctgtac ggtggcggga
     6181 gtaccggagg gttgatcaac atcgtgacca aaaaaggcca gccggaaacc atgatggagt
     6241 ttgaggctgg cacaaaaagt ggctttaaca gcagtaaaga tcacgatgag cgcattgccg
     6301 gtgctgtctc cggcggaaat gaccatatct ccggacgtct ttccgtggca tatcagaaat
     6361 ttggcggctg gtttgacggt aacggcgatg ccaccctgct tgataacacc cagaccggcc
     6421 tgcagcactc caatcggctg gacatcatgg gaaccggtac gctgaacatc gatgaatccc
     6481 ggcagcttca actgataacg cagtactata aaagtcaggg ggacgacaat tacgggctta
     6541 atctcgggaa aggcttttcc gccatcagcg ggagcagcac accatacgtc agtaaggggc
     6601 tgaattctga ccgcattccc ggcactgagc ggcatttgat cagcctgcag tactctgaca
     6661 gtgatttcct gagacaggaa ctggtcggtc aggtttacta ccgcgatgag tcgttgcggt
     6721 tctacccgtt cccgacggta aatgcgaata aacaggcgac ggctttctcc tcgtcacagc
     6781 aggataccga ccagtacggc atgaaactga ctctgaacag ccaactgatg gacggctggc
     6841 aaatcacctg ggggctggat gctgagcatg agcgctttac ctccaaccag atgttcttcg
     6901 atctggctca ggcaagtgct tccggagggc tgaacaacca taagatttac accaccgggc
     6961 gctatccgtc atatgacatc accaatctgg cggccttcct gcaatccagc tatgacatta
     7021 atgatatttt taccgtcagc ggtggcgtac gctatcagta tactgagaac agggtagatg
     7081 attttatcga ctacacgcag caacagaaga ttgctgccgg gaaggcgata tctgccgacg
     7141 ccattcctgg tggttcggta gattacgata actttctgtt caatgctggt ctgctgatgc
     7201 acatcaccga acgtcagcag gcatggttca atttttccca gggggtggca ttgccggatc
     7261 cggggaaata ttatggtcgc ggcatctatg gtgcagcagt gaacggccat cttcccctga
     7321 caaagagcgt gaacgtcagc gacagtaagc tggaaggcgt gaaagtcgat tcttatgaac
     7381 tgggctggcg ctttaccggt gacaacctgc ggactcaaat cgcagcatat tactcgcttt
     7441 ccaataagag cgtggaaagg aataaagatc tgaccatcag tgtgaaggac gacaggcgcc
     7501 gtatttacgg cgtggaaggt gcggtggact acctgatccc ggatactgac tggagtaccg
     7561 gtgtgaactt caatgtgctg aaaaccgagt cgaaagtgaa cggtcaatgg caaaaatatg
     7621 acgtgaagga atcaagtcca tcgaaagcga cagcttacat tagctgg
//
LOCUS       DAZKHC020000165         7644 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZKHC020000165 DAZKHC020000000
VERSION     DAZKHC020000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.165"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            329..1969
                     /gene="trg"
                     /locus_tag="ACHOSM_RS22300"
                     /old_locus_tag="pgaptmp_004460"
     CDS             329..1969
                     /gene="trg"
                     /locus_tag="ACHOSM_RS22300"
                     /old_locus_tag="pgaptmp_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HGU4560819.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFESIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESARLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAALS
                     LEEQAARLTEAVDVFRLHKHSVSAKPRGAGEPVSFATV"
     gene            complement(2007..2930)
                     /locus_tag="ACHOSM_RS22305"
                     /old_locus_tag="pgaptmp_004461"
     CDS             complement(2007..2930)
                     /locus_tag="ACHOSM_RS22305"
                     /old_locus_tag="pgaptmp_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4560820.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRISD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3147..4490
                     /locus_tag="ACHOSM_RS22310"
                     /old_locus_tag="pgaptmp_004462"
     CDS             3147..4490
                     /locus_tag="ACHOSM_RS22310"
                     /old_locus_tag="pgaptmp_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4560821.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIGDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLCNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4715..6370
                     /gene="opgD"
                     /locus_tag="ACHOSM_RS22315"
                     /old_locus_tag="pgaptmp_004463"
                     /gene_synonym="mdoD"
     CDS             4715..6370
                     /gene="opgD"
                     /locus_tag="ACHOSM_RS22315"
                     /old_locus_tag="pgaptmp_004463"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HGU4560822.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6510..6734
                     /locus_tag="ACHOSM_RS22320"
                     /old_locus_tag="pgaptmp_004464"
     CDS             6510..6734
                     /locus_tag="ACHOSM_RS22320"
                     /old_locus_tag="pgaptmp_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HGU4560823.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6797..7333
                     /gene="rimL"
                     /locus_tag="ACHOSM_RS22325"
                     /old_locus_tag="pgaptmp_004465"
     CDS             6797..7333
                     /gene="rimL"
                     /locus_tag="ACHOSM_RS22325"
                     /old_locus_tag="pgaptmp_004465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HGU4560824.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7328..>7644)
                     /locus_tag="ACHOSM_RS22330"
                     /old_locus_tag="pgaptmp_004466"
     CDS             complement(7328..>7644)
                     /locus_tag="ACHOSM_RS22330"
                     /old_locus_tag="pgaptmp_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560825.1"
                     /translation="DAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLD
                     EHVVIQGESRISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAG
                     LL"
BASE COUNT         1981 a         1805 c         1960 g         1898 t
ORIGIN      
        1 tttgatagaa atatcggtcg gggccttcgt ctttctgatt cccggttagc ctgaaaacag
       61 aaagtctcag gcacccgcag gcatcctatg aggtttcctt agggacgaaa ataatcactt
      121 cacgaaattg cgtgctgttt tccagaattt ttcgtcattc gggttagccc gtttaatcat
      181 tcgttactct cttcattcca atggcattaa ccttctctgc aataattgtt gtaaaaatgt
      241 gacgcaaaga ggcttttagt cgtaagtaat taccgtcaag tgccgatgac tttctatcag
      301 gagtaaacct ggacgagaga caacggtaat gaatacaact ccctcacagc gattaggttt
      361 tttgcatcac atcaggctgg ttccgttatt tgcctgcatt cttggcggta tcttagttct
      421 attcgcatta agttctgctc tggctggcta tttcctctgg caggccgatc gcgatcagcg
      481 tgatgttact gcggagattg agattcggac cgggttagcg aacagttcag attttttgcg
      541 ttcagcccgg atcaatatga ttcaggccgg ggctgcgagt cgtattgcgg aaatggaagc
      601 aatgaagcga aatattgcgc aagccgaatc ggagattaaa cagtcgcagc aaggttatcg
      661 tgcttatcag aatcgaccgg tgaaaacacc tgctgatgaa gccctcgaca ctgaattaaa
      721 tcaacgcttt caggcttata tcacgggtat gcaacctatg ttgaaatatg ccaaaaatgg
      781 catgtttgaa tcaattatca atcatgaaag tgagcagatc cgaccgctgg ataatgctta
      841 taccgatatt ttgaacaaag ccgttaagat acgtagcacc agagccaacc aactggcgga
      901 actggcccat cagcgcaccc gcctgggtgg gatgttcatg attggcgcgt ttgtgcttgc
      961 cctggtcatg acgctgataa catttatggt gctacgtcgg atcgtcattc gtccactgca
     1021 acatgccgca caacggattg aaaaaatcgc tagtggcgat ctgacgatga aggatgaacc
     1081 ggcgggtcgt aatgaaatcg gtcgcttaag tcgtcattta cagcaaatgc agcattcact
     1141 ggggatgaca gtagggactg ttcgacaggg tgcggaagag atttatcgtg gcaccagcga
     1201 aatttcagct ggcaatgcgg acctgtcatc tcgcaccgaa gaacaagcgg cggctatcga
     1261 acaaactgcc gctagcatgg agcaactcac tgcgacagtg aaacagaatg cggataacgc
     1321 gcatcatgcc agcaaactgg cgcaagaggc ttctattaaa gccagcgatg gcgggcagac
     1381 ggtttccggt gtagtaaaaa cgatgggcgc tatctctaca agttcgaaga aaatttccga
     1441 gatcaccgcc gtcatcaaca gtattgcttt ccagacgaat attctggcac tgaatgctgc
     1501 cgttgaagcc gcgcgagcgg gtgagcaagg ccgtggattt gccgttgtcg ccagcgaagt
     1561 acggacactc gcaagccgca gcgcccaagc ggcgaaagag attgaaggct tgatcagtga
     1621 atcagccagg ttaattgacc tggggtcgga tgaggtggca acggcaggga aaaccatgag
     1681 cactattgtt gatgccgtcg cgagtgtcac acatatcatg caggaaatcg ccgccgcctc
     1741 ggatgaacaa agtagaggca taacgcaggt tagccaggcg atttctgaaa tggataaggt
     1801 gacgcaacag aatgcttctc tggtagaaga ggcctcagcg gcggcgttgt cccttgaaga
     1861 acaggcggca cgattaactg aggcggtgga tgtattccgt ctgcacaaac attctgtgtc
     1921 ggcaaaacct cgcggagcgg gtgaaccagt tagtttcgct acggtgtgaa aatgttcaag
     1981 gagggatcga cagatccctt cacctttcag aacggcattg attttcgaat agcgttaatc
     2041 atcaactggc aaccagaaga gaacgtcgca tctacgcggg tcagtattcc aatcggttcg
     2101 cctgcaccat gtcccggaac aggcagggcc accagcgtgg catgacgcag gtcgtctttt
     2161 acagcgccag aagggacaaa ccacacgtag tcgtattcaa ccgtaagttg acgagatagc
     2221 gaagcagaca gcgtttcgat acaacccgaa ggaattttac atccctggct ctgtactaat
     2281 gcatctgaat gctggcgtgg cgcagtgcct tctggtgata caacgaccgg ccattccagc
     2341 acccggctta gcgttacgtt ctcctgaagt agcgggtgat tagggcggac gaccagcttc
     2401 agcgattcaa gaaacagcag ttcgtaatta agcccggtca tcagttcagg atctgatatc
     2461 cgaccaatgc cgatatcgat ttccccggtt ttcaaacccg ccagaatcat agggttactc
     2521 attgtcgcaa cttgcaaggt cgtctctttt tgttgctgat gaaactgacc tataaccgaa
     2581 ggtaatatcc ccagtgccgc agtaggtagt gcaccaaccc tgacgacatc attattaaga
     2641 ccttctttac gatgaagcga ctgtccggca gtgttgatgg cgtcaagaac tctgactgca
     2701 tgcgttaaaa attgttcgcc gggtaaggta agttgcgccc cctgacgacc acgctcaaac
     2761 aagcgagcgc ccgtcagctg ctccagttca ttcaatgtct tagagagcgc aggttgactc
     2821 aaattaaggg tttcagccgc gcgccccaaa gttccttgtt gtgcgacagc tacgaatgta
     2881 tgaaggtggc gcaaacggat gcgctgacta aacagactat ttttttccat aagcgatgtt
     2941 aaaaacgaag cggtgtcgct gacaagtgaa gttgtttgat tatgataact tgattgcaaa
     3001 atattattaa caattaaagc aattgtgtta cagcaaaatg gataatattg atgttttcgc
     3061 ggcgagatca cagtttgtaa attcttcccg ccagagtgaa tgcggttacc tacactccag
     3121 attactgacc actggaggca gacactatgg cgaacagcat cacggcggat gagattcggg
     3181 aacagttttc gcaggcaatg tcagccatgt accagcaaga agttccgcag tacggcacgc
     3241 tgctggaact ggtagctgat gtgaatctgg ctgtgctgga aaacaatcct caactgcacg
     3301 aaaaaatggt aaatgcagac gagctggcgc gactgaatgt tgaacgtcat ggggcgattc
     3361 gcgttgggac tgcacaagag cttgctactc ttcggcggat gtttgccatt atggggatgt
     3421 acccggtgag ctattacgat ctctcgcagg caggggtgcc ggtacattcg acagcatttc
     3481 ggcccattgg tgatgcttct ctggcgcgta atcccttccg cgtttttacc tccttactcc
     3541 gccttgagct tatcgagaac gaaattttgc gccagaaagc ggcggagatt ctgcgtcagc
     3601 gcgatatctt caccccacgt tgtcgacaac tgttagagga atatgagcag cagggcggtt
     3661 ttaacgaaac acaggcacag gagtttgtgc aggaagccct ggaaacgttt cgctggcacc
     3721 agtcagcaac ggtagatgaa gaaacctatc gcgccttgca caacgaacat cggttgattg
     3781 ctgatgtggt ctgttttcct ggatgccata tcaaccatct gacgccacgt acgctggata
     3841 ttgaccgggt gcagtcgatg atgcctgaat gcggaattga accaaaaatt ctgatcgaag
     3901 ggccgccgcg ccgcgaggta ccgattttac tacgccagac cagctttaaa gcactggaag
     3961 agacggtgtt gtttgcgggg cagaaacagg gcacgcatac tgcgcgcttt ggtgaaatag
     4021 agcagcgtgg tgtggcatta acgccgaaag ggcgacaact gtatgatgat cttctgtgta
     4081 acgctggaac cgggcaggat aatctcactc accaaatgca tttacaggaa accttccgca
     4141 cttttcctga cagtgagttt ttaatgcgtc agcaagggtt ggcatggttc cggtaccgtc
     4201 tgacgccttc gggtgaggcg catcgtcagg cgattcatcc tggagacgat ccacagccct
     4261 taattgaacg tggttgggta gtggcgcaac ccatcaccta tgaagatttc ttgcccgtta
     4321 gcgcggcggg gatcttccag tcaaatctgg gtaatgaaac gcagacacgc agtcacggta
     4381 atgccagtcg cgaagcattt gagcaggcgt tgggttgtcc ggttttggat gagttccagc
     4441 tttaccagga agcggaagaa cgcagtaaac gtcgctgtgg tttgctttaa attctgacca
     4501 tccgcctttg caaaaatttg cctgatttta caaacgaatc aggctcatcc catcgacata
     4561 aaaaaaatgc cgatttatgc atattctctc agttcaacaa ttggattatt aataaatatt
     4621 gtctagagtg agcggtcata aataagcact ttcttgccgc tgaaaacgac cagcgcggga
     4681 ccattcacaa caccagaagg actcactttc aggtatggat cgtagacgat ttattaaagg
     4741 ttcaatggct atggccgccg tgtgcggtac cagcggcatt gcttctcttt tttctcaggc
     4801 ggcattcgcg gcagattctg atattgccga cgggcaaacc cagcgttttg acttctccat
     4861 tctacagtca atggcgcacg acttagcgca aacagcgtgg cgtggtgcgc ctcgtccgtt
     4921 acctgacacg ctggcgacaa tgacgccgca ggcttataac agtattcaat acgacgccga
     4981 aaaatcgctc tggcataacg ttgagaaccg tcaactggac gctcagttct tccatatggg
     5041 aatgggattc cgtcgccgcg ttcgtatgtt ttctgtagat ccagcaacac atctggcgcg
     5101 tgaaattcac tttcgcccgg agttgttcaa atacaacgat gcaggtgttg ataccaaaca
     5161 attagaaggg caaagcgatc tcggctttgc cggttttcgc gtgtttaaag cccccgaact
     5221 ggcgcgccgt gatgtagtat catttctcgg cgcgagttat ttccgcgccg ttgatgatac
     5281 atatcaatac ggtttgtcgg cccgcggcct ggcgatcgac acttacaccg acagtaaaga
     5341 agagttcccc gactttaccg ccttctggtt tgatacggta aaaccggggg caactacctt
     5401 taccgtttat gcgttgctcg atagcgccag cattactggt gcctataagt tcactatcca
     5461 ttgtgagaaa agtcaggtga ttatggatgt ggaaaatcac ctgtatgcgc gcaaagacat
     5521 taaacagctg ggcattgcgc cgatgaccag tatgttcagc tgcggtacta atgaacgtcg
     5581 gatgtgcgat acaattcatc cgcaaattca tgactctgat cgtctgtcca tgtggcgggg
     5641 caacggcgag tggatttgcc gtccgctgaa taatccgcaa aaattgcagt tcaatgctta
     5701 caccgacaac aacccgaaag ggtttggttt attgcaactg gatcgtgatt tctcccatta
     5761 tcaggacatt atgggctggt ataacaaacg cccaagtctg tgggtggagc cgcgtaacaa
     5821 gtggggtaag ggcaccatcg gcctgatgga aatcccaaca acgggtgaaa cgctggataa
     5881 cattgtctgc ttctggcagc cagaaaaagc tgtaaaagcg ggtgatgagt ttgcattcca
     5941 gtatcgtctg tactggagtg cgcaaccgcc tgttcattgc ccattagcgc gcgttatggc
     6001 gacgcgtacc ggcatgggcg gtttcccgga aggttgggcg ccaggtgaac actatcccga
     6061 aaaatgggcg cgtcgttttg ccgtcgattt cgttggtggt gatctgaaag ctgccgcgcc
     6121 aaaaggcatt gagccggtga ttacgctttc cagtggggaa gcgaagcaaa tcgaaattct
     6181 ctatattgaa cccatcgatg gttatcgtat tcagtttgac tggtatccga cttcggactc
     6241 cactgatccg gtcgatatgc ggatgtatct acgttgtcag ggggacgcta tcagtgaaac
     6301 atggctgtat cagtatttcc cgccagcgcc ggataaacgt cagtatgttg acgaccgcgt
     6361 gatgagttaa tcgttttttc ttcggcacct tcttcgggag gtgccgtctg gttaaacacg
     6421 atcccgctcg catttttccc taagttaaat gagtaatctg atggtgtgta tttcagatac
     6481 accttgtcag ccactaacag ggagtgcgta tgtttccaga ataccgagat ttaatatccc
     6541 gtctgaaaaa cgaaaatcct cgctttatgt ccttgttcga taaacacaat aaacttgatc
     6601 atgaaattgc cagaaaggaa ggttccgacg gtcgagggta caatgcggaa gtggtccgca
     6661 tgaaaaaaca aaagctacag ttaaaagatg agatgctcaa aatcctgcag caggagagcg
     6721 tcaaagaggt gtaaactttc ctaagccgcc agccaggcgg cttttttaac aacagcatgg
     6781 attgactgga gataagatga ctgaaacgat aaaagtaagc gaatcacttg aattacatgc
     6841 tgttgcggaa agtcacgtca cacctcttta tcagttaatc tgtaaaaata aaacctggtt
     6901 acagcagtcg ctaaactggc cgcagttcgt tcagagtgaa gaggacacgc gaaaaacggt
     6961 gcagggtaat gtgatgttgc atcaacgcgg ctatgccaaa atgttcatga ttttcaaaga
     7021 agatgaactt atcggcgtta tctcgtttaa tcgtattgaa ccactgaata aaactgctga
     7081 aatcggctac tggctggacg aatctcatca ggggcagggg atcatttctc aggcgctgca
     7141 ggcattgatt catcattacg ctcagtctgg tgaacttaga cgcttcgtga tcaaatgtcg
     7201 ggtggacaat ccgcaaagca accaggtcgc tttgcgcaat ggttttatcc ttgaaggttg
     7261 cctgaaacag gctgagttcc tgaatgatgc ctatgatgat gtgaacttat acgcgcgtat
     7321 tatcgattca taataaccct gccagcggcg ttcgcgtaat gcgctcctcg ccgttaatga
     7381 cttttggacc acggacatga accgtgtcgc catcaaaggc ttcaacgacg gcgtggtcgg
     7441 ttaactccac atggttttca attatcaccg cacctgaaat acggctctca ccttgtatta
     7501 caacgtgctc gtcgagcaga atcggtccac cacgtaccac ggcatttccg ccaatcaaga
     7561 catgatgttt taacacacaa ttaccttcca caatggcata ttccgccacc tgcgaactgt
     7621 aatgaatcgt cggaatggca tctt
//
LOCUS       DAZKHC020000166         7551 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZKHC020000166 DAZKHC020000000
VERSION     DAZKHC020000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.166"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            12..866
                     /gene="gatY"
                     /locus_tag="ACHOSM_RS22335"
                     /old_locus_tag="pgaptmp_004467"
     CDS             12..866
                     /gene="gatY"
                     /locus_tag="ACHOSM_RS22335"
                     /old_locus_tag="pgaptmp_004467"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HGU4560826.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            895..2162
                     /gene="gatZ"
                     /locus_tag="ACHOSM_RS22340"
                     /old_locus_tag="pgaptmp_004468"
                     /pseudo
     CDS             895..2162
                     /gene="gatZ"
                     /locus_tag="ACHOSM_RS22340"
                     /old_locus_tag="pgaptmp_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            2172..2624
                     /gene="gatA"
                     /locus_tag="ACHOSM_RS22345"
                     /old_locus_tag="pgaptmp_004469"
     CDS             2172..2624
                     /gene="gatA"
                     /locus_tag="ACHOSM_RS22345"
                     /old_locus_tag="pgaptmp_004469"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HGU4560827.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2655..2939
                     /gene="gatB"
                     /locus_tag="ACHOSM_RS22350"
                     /old_locus_tag="pgaptmp_004470"
     CDS             2655..2939
                     /gene="gatB"
                     /locus_tag="ACHOSM_RS22350"
                     /old_locus_tag="pgaptmp_004470"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HGU4560828.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            2943..4298
                     /locus_tag="ACHOSM_RS22355"
                     /old_locus_tag="pgaptmp_004471"
     CDS             2943..4298
                     /locus_tag="ACHOSM_RS22355"
                     /old_locus_tag="pgaptmp_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGU4560829.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQILPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            4346..5386
                     /gene="gatD"
                     /locus_tag="ACHOSM_RS22360"
                     /old_locus_tag="pgaptmp_004472"
     CDS             4346..5386
                     /gene="gatD"
                     /locus_tag="ACHOSM_RS22360"
                     /old_locus_tag="pgaptmp_004472"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU4560830.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            5492..6265
                     /locus_tag="ACHOSM_RS22365"
                     /old_locus_tag="pgaptmp_004473"
     CDS             5492..6265
                     /locus_tag="ACHOSM_RS22365"
                     /old_locus_tag="pgaptmp_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4560831.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETENQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6347..7246)
                     /gene="yegS"
                     /locus_tag="ACHOSM_RS22370"
                     /old_locus_tag="pgaptmp_004474"
     CDS             complement(6347..7246)
                     /gene="yegS"
                     /locus_tag="ACHOSM_RS22370"
                     /old_locus_tag="pgaptmp_004474"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HGU4560832.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1911 a         1691 c         1955 g         1994 t
ORIGIN      
        1 aggatatgaa aatatacgtg gtatcgacaa agcagatgct gaacaacgca cagcgcggcg
       61 gttatgcggt tccggcattc aatattcaca atctcgaaac gatgcaagtg gtggtggaaa
      121 ccgctgccaa cctgcatgcg ccggtcatca tcgccggaac gcctggcaca tttactcatg
      181 ctggtacaga aaatctgttg gcgctggtca gcgcgatggc gaagcaatat caccatccac
      241 tggcaattca tctcgaccat cacacgaaat ttgacgatat cgctcagaag gttcgttctg
      301 gcgtgcgctc agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat atttcacggg
      361 tcaaagaggt ggtggatttt tgccatcgct ttgatgtcag cgtcgaagcg gagctggggc
      421 aacttggcgg ccaggaagat gatgtgcaag tcaatgaagc cgatgctttt tataccaacc
      481 ccgctcaggc gcgtgaattt gccgaggcaa ccggaattga ttccctggcg gtcgccatcg
      541 gcacggctca tgggatgtat gccagcgcac cggcgcttga tttttctaga ctggagaaca
      601 ttcgccagtg ggtgaactta ccgctggtgc tgcatggcgc gtcagggtta tcgactaagg
      661 atattcagca aaccatcaaa ctggggatat gcaaaatcaa cgttgcaacg gagctgaaaa
      721 atgccttctc gcaggcgtta aaaaattacc tgaccgagca ccctgaagcg accgatcccc
      781 gggattattt gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa gtgattgccg
      841 attgtggctg cgagggcagg gcataacgca cctgccattt aataaggaaa aaacatgaaa
      901 acgttaattg cccggcataa agctggtgaa catatcggca tatgttcagt ctgttctgcc
      961 catccgttgg ttatcgaagc ggcgctggca tttgatcgca acagcacgcg caaagtgctg
     1021 attgaagcaa cgtcaaacca ggtcaatcaa tttggcggtt ataccggaat gacaccggca
     1081 gactttcgcg aatttgtttt tacgattgcc gataaagttg ggtttgcacg cgaacgcatt
     1141 attctcggcg gcgatcatct ggggccaaac tgctggcagc aagaaaatgc ggatgcggcg
     1201 atggaaaaat ccgtcgagct ggtaaaggaa tatgttcgtg ccggcttcag taaaattcat
     1261 cttgatgcgt caatgtcctg cgcgggggat cccataccgt tagcaccaga aacggttgcg
     1321 gaacgagctg ctgtgctttg ctttgctttg ctgcggaaag tgtggcgaca gattgccagc
     1381 gtgagcaact gagctatgtc attggcaccg aagttccggt tccgggcggt gaggccagcg
     1441 ccattcagtc agtacacatc acccatgttg aagatgccgc caatacttta cgtacgcatc
     1501 aaaaggcctt tattgcccgt gggctgacag aggcgttaac acgcgtgatt gccatcgtgg
     1561 tgcagccggg cgtggaattt gatcacagca atattatcca ttatcagccg caggaagcgc
     1621 agccgctggc gcaatggata gaaaacaccc gaatggttta tgaagcacat tctaccgatt
     1681 accagacccg gacggcttat tgggaattag tccgcgatca ctttgcaata ttgaaagtcg
     1741 gtcccgcatt aacctttgct ttacgcgagg cgatatttgc actggcacaa attgagcagg
     1801 aacttatcgc ccctgaaaat cgcagcggtt gcctggcggt aattgaagaa gtgatgctcg
     1861 acgaaccgca atactggaaa aaatattatc gtacgggttt taacgattca ttactggata
     1921 ttcgttacag cctgtcggat cgtattcgtt attactggcc gcatagtcgg attaaaaata
     1981 gcgtcgaaac gatgatggtg aatcttgaag gcgtggacat cccactgggc atgattagtc
     2041 agtatcttcc caaacaattt gaacgcattc agtccgggga attatcagca ataccgcatc
     2101 agctgattat ggataaaatt tatgatgttt tgcgcgccta tcgctacggc tgtgcggaat
     2161 aaggacggta tatgactaac ctgtttgttc gtagcggaat ttcttttgtc gatcgtagcg
     2221 aagttttaac ccatatcggt aatgagatgc tcgccaaagg tgtggttcat gatacctggc
     2281 cacaggcatt aattgccaga gaagcagaat tccctaccgg gataatgctt gagcagcacg
     2341 ccattgcaat accgcattgt gaggcgattc atgctaagtc gtcagccatt tatctgttaa
     2401 ggccaacaaa taaagttcat tttcagcaag cggatgatga taacgacgtg gaggtatcgt
     2461 tggttattgc gttgattgtg gaaaatccgc agcagcaatt gaaactttta cgctgtttat
     2521 ttggcaagtt acaacagccc gatatcgtcg agacactaat cactcttcct gaaacccagt
     2581 taaaggaata cttcacaaag tatgttttag attcagacga ataaatccct ctgtaacaat
     2641 aaaaaggact atttatgaaa cgcaagatta ttgtcgcttg cggaggcgcg gttgcgacct
     2701 ctacgatggc ggcggaagaa attaaagagt tgtgtcagaa tcataatatt cctgttgaat
     2761 taatccagtg tcgggttaat gaaatagaaa cctatatgga tggtgtgcat ttgatatgca
     2821 ccactgccaa agtggatcgt agttttggcg atattccgtt agttcacggc atgcctttta
     2881 tttctggtgt cggtatcgaa gcattacaaa ataaaattct gactatctta caggggtgac
     2941 ctatgttttc agaagtcatg cgttatattc tcgacctcgg ccctacggtg atgcttccga
     3001 ttgtcatcat tattttttct aaaatattag gcatgaaggc aggcgattgc tttaaagcgg
     3061 gtctgcatat cgggattggc tttgttggca ttggccttgt gattggctta atgctggatt
     3121 ccattggtcc ggcggcgaaa gcgatggcgg aaaatttcga cctgaatctg catgtggtcg
     3181 atgtcggctg gccgggctct tcaccaatga cctgggcgtc gcaaattgcg ctggtggcga
     3241 ttccgattgc gattctggtt aacgtggcga tgttactgac ccgtatgacg cgggtggtaa
     3301 atgttgatat ctggaatatc tggcatatga ccttcaccgg cgcgttgctg catctggcaa
     3361 ccggttcatg gatgataggg atggcaggtg tggtaattca cgcggcgttt gtttataagc
     3421 tcggcgactg gtttgcccgc gatacccgaa atttctttga gctggaaggt attgctattc
     3481 cgcacggtac gtcggcgtat atggggccga ttgcggtgct ggtcgatgct atcatcgaga
     3541 aaatcccagg cgttaaccga attaaattta gcgccgacga tattcagcgc aaatttggtc
     3601 catttggcga gcctgtcacc gtgggttttg tgatggggct gattatcggc atcctcgcgg
     3661 gttacgatgt caaaggtgta ttgcagctgg cggtaaaaac ggcggcagtt atgctgctga
     3721 tgccacgggt gattaaaccc atcatggatg gtttaacgcc catcgctaag caggcccgta
     3781 gtcgtttaca ggcgaagttc ggcggtcagg agttcctgat tggcctggat cccgcattac
     3841 tgctggggca tacggcggtg gtatcggcaa gcctgatttt tatcccgctc accattttaa
     3901 ttgctgtttg tgttccgggt aatcagatac tgccgtttgg cgatcttgcc accatcggct
     3961 tcttcgtggc gatggcggtc gcggtgcatc gtggaaatct gttccgcacc ttaatctcgg
     4021 gtgtcatcat tatgagcatc accctgtgga tcgcgacgca aactattggt ttgcacaccc
     4081 aactggcggc taatgctggg gcgttaaaag ccgggggtat ggtggcttca atggatcagg
     4141 gcggttctcc cattacctgg ttactgattc aggttttctc cccgcaaaat attcccggtt
     4201 tcattattat cggcgcaatt tatcttaccg gtattttcat gacctggcgt agagcgcgtg
     4261 gttttattaa acaagagaaa accgttctcg cagaataatt tttacctgag ggggaggtta
     4321 tcccccttaa caatcaggag tttttatgaa atcaatggtg aatgatactg atggtatagt
     4381 gcgcgttgca gaaagcgtca ttcctgaaat taaacatcag gatgaggtgc gggtaaaaat
     4441 tgccagctcg ggcttatgtg gttccgattt acccaggata tttaaaaatg gtgcacatta
     4501 ttatccaata acgttaggcc atgaatttag cggctatatt gatgcggtgg gatccggtgt
     4561 tgatgattta caccctggcg atgcggttgc ctgtgtgccg ttattaccct gttttacttg
     4621 tccagagtgt ttgaaagggt tttattccca gtgcgcaaaa tatgatttta ttggctcgcg
     4681 gcgtgatggt ggatttgctg aatatattgt cgttaagcga aaaaatgtct ttgctctacc
     4741 cacggatatg cctattgagg atggggcttt tattgagccg attaccgttg gcctgcatgc
     4801 ttttcattta gcgcaaggtt gtgagaataa aaacgttatt attattggtg ccggaaccat
     4861 tggcctgctg gctattcagt gcgctgtcgc gctgggagca aagagtgtga cggcgatcga
     4921 cattagctca gaaaaactgg cactggcaaa atctttcggt gcgatgcaaa catttaacag
     4981 tagcgaaatg agcgcgccgc aaatgcagag cgttttacgc gaactgcgct ttaatcagct
     5041 tatcctcgag acggctggcg taccgcaaac tgtcgaactg gcggtagaga ttgccggtcc
     5101 tcatgcccaa ctggcgctgg tgggcacgtt gcatcaggat ctgcatttaa catcggcaac
     5161 gtttggcaaa atattgcgta aagagctgac ggttatcggc agttggatga actactccag
     5221 cccttggccg gggcaggagt gggaaacggc gagccggttg ctgacagaac gtaagttaag
     5281 cctggagcca ttaatcgctc accgtggaag ctttgaaagc ttcgcccagg cggtgcgtga
     5341 catcgctcgt aatgccatgc cgggcaaagt gttgctcatt ccctgaaacc gcgggccagc
     5401 gtgatgctgg cccggtattg tgcaaaacag atcattcacc aatggtcccc cttcgtttac
     5461 actagccaca attgaatatg ggtaaatgac gatgaattca ttcgagcgaa ggaataagat
     5521 catccaatta gtgaatgaac agggaaccgt gcttgttcag gatctggcgg gagtatttgc
     5581 tgcctcggaa gcgacaatcc gtgccgattt gcgctttctc gaacaaaaag gcgtggttac
     5641 gcgctttcat ggcggtgcgg cgaaaataat gtccggtaat agtgaaaccg agaaccagga
     5701 agtcgggttt aaagagcgat ttcagctcgc cagcgcgccg aaaaacagaa tagcgcaggc
     5761 ggcagtcaaa atgatccatg aagggatgac cgttattctc gacagcggaa gtaccacaat
     5821 gcttatcgcc gaaggattaa tgaccgccaa aaatatcacg gtgattacca acagtctccc
     5881 ggctgcgttt gccctttccg aaaataaaga cattactctg gtcgtctgcg gtggcacggt
     5941 ccgccataaa acgcgatcta tgcatggatc tattgctgaa cgttccctgc aggatattaa
     6001 tgccgattta atgtttgtcg gtgctgatgg tattgatgcg gttaatggta ttacgacctt
     6061 taatgagggt tattccgtta gcggtgcgat ggtcacagcc gctaataaag tgattgccgt
     6121 tctcgattca tcgaaattta accgtcgcgg ttttaatcag gtactgccaa tcgaaaaaat
     6181 tgacattatt attaccgatg atgctgtgtc tgaggtggat aaactggcat tgcagaagac
     6241 acgggtgaaa ttaattacgg tgtagtattg ttctgtatgc cggataaggt atttacaccg
     6301 catccggcat tacggattct ccctggttca agagagaata gtttgattaa cgcaataatg
     6361 gacaatctgg tggtaatcga caacgcaacg ccgccggaag tatttcaata tgaaagtttt
     6421 gtccactcaa cggttcgcca tcaagattaa aagtgatttc gtgtggggct tgtatatcaa
     6481 accacgacga agcgccttcg ataatattcg ggttatcttc gtcagatttt aaagttgata
     6541 cgagagccgg aagaatttca tcgccggtaa aaatgcgcag ttgcagcaag ccatcgttaa
     6601 ttaacgcgtt cgggcacaat tgttgaccgc caccggcctg acgcccgtta ccaataccaa
     6661 tgaccagggc gtcaccttgc cagtgaaagt tttcaccgcg gatttcacaa cggtccggtt
     6721 gcagagtatc catacgcatt aagccatgaa tgatgtatga gacgccaccc agcgcggctt
     6781 ttaatttttc cggcgtttct gtggtaatac gcgtcccaaa cccgcctgtc gccatattaa
     6841 taaaacaggt ttgtttgttg acctgcgcca tatctatcgc aatggcatta ccggcaattg
     6901 ccagtttcag cgccttatcc agtgcctcag gaatccctac actggtggca aaatcattgg
     6961 cggttcctaa tggcaaaatt cccagcgcgg gtatgtcatc cccctcacac tgaatcaacg
     7021 ccgtagaaac ttcattaatg gtgccatcgc caccaccggc aatcaccgtt gcgacgccca
     7081 acttccgggc ctcctctaca aatcgtgcgg catcaccttt ctcccaggtg acccgcacat
     7141 ggatcgtcat tccttcctca cgcaacagca taattgcttc gcgcaagggt agattgtcag
     7201 tacttttgcc attaagaatc agtaagctgg cgggaaattc tgccatgatc gtatgtgcct
     7261 ttatgattgg tctgtagata gtgtagagca gaaaagaaaa ggtggagtca gaacaggatg
     7321 aaagtcggag gataaaaagt cagttcaagt atggaggatt cagtggttac caccaggagc
     7381 ttgcaatggg aacagcgaat attaacggta accaccgata aatttcaaaa aagagcatat
     7441 gcctaatatt caaccaaaca gtggcatctt aaatatacta ttccaaatat attaaagccc
     7501 catggagtta cctggaaagg gccttcatgt ctgtaattcc tacttatgta g
//
LOCUS       DAZKHC020000167         7522 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZKHC020000167 DAZKHC020000000
VERSION     DAZKHC020000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.167"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..4826)
                     /locus_tag="ACHOSM_RS22375"
                     /old_locus_tag="pgaptmp_004475"
     CDS             complement(<1..4826)
                     /locus_tag="ACHOSM_RS22375"
                     /old_locus_tag="pgaptmp_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase toxin
                     domain-containing protein"
                     /protein_id="HGU4560833.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDKDGAINTGSKNVNINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKDLDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLNFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPNDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVNVTGAEGYYNATLDYGDGCTTVTDGKGIHRYYYDPD
                     GNILREEAPDGSTTMYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGAEWQ
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLRWQQHHKLSG
                     LERTGTGAEGFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYLYVLDNCGRVTEEHDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELAEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEEATWGGLVPSLVREYRYDSA
                     GNVSAVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGNESYGYDSCGYLKAQ
                     SAGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSR
                     DQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYS
                     VRHLVFNSFELISQQFSRVRQPHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTGYPDPRGELDPEADPGLLYAGQWQDAESGLCYNRFRYYEPE
                     TGMYLVSDPLGLQGGEQTYRYVPNPCGWVDPLGLAASSKISSLMDYIGDGRRVSGHTG
                     FLDGVRLSRSQINNIAKEMEKLGIKVIRKADKYLPPNARAAFDYGLRNIYLRKNATLY
                     EVYHEVIHAKQFAKIGREAYEALGRLSREEHVLNEILKSKNLFNEAEIAHAIKYVEG"
     gene            complement(4846..5307)
                     /locus_tag="ACHOSM_RS22380"
                     /old_locus_tag="pgaptmp_004476"
     CDS             complement(4846..5307)
                     /locus_tag="ACHOSM_RS22380"
                     /old_locus_tag="pgaptmp_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HGU4560834.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQILVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5335..7236)
                     /locus_tag="ACHOSM_RS22385"
                     /old_locus_tag="pgaptmp_004477"
     CDS             complement(5335..7236)
                     /locus_tag="ACHOSM_RS22385"
                     /old_locus_tag="pgaptmp_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU4560835.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGTQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1425 a         2272 c         2002 g         1823 t
ORIGIN      
        1 ccctcaacat attttatggc atgagctatt tccgcttcat tgaataaatt tttacttttt
       61 aatatttcat ttagaacgtg ttcctcccta gataaacgtc ctagtgcttc gtatgcttct
      121 cgtccaattt tcgcaaattg cttagcatga atcacttcat gatacacctc atataaggta
      181 gcatttttcc taagataaat attgcgaagg ccataatcaa aagctgccct agcatttggt
      241 ggcaaatatt tatctgcttt ccttattact ttaattccta gcttctccat ttctttagca
      301 atattgttta tttgactacg tgataaacga accccatcca ggaaacccgt atgcccacta
      361 acacgacgac catcgccaat atagtccatc aaactgctga ttttagaact tgcagccaat
      421 cccagcggat caacccaccc acaaggattc ggcacatacc ggtaagtctg ctcccctccc
      481 tgcaacccca gcggatcact caccaggtac atcccggttt ccggctcgta ataccggaac
      541 cggttatagc acagccccga ttccgcatcc tgccactgtc ccgcatacag caggccgggg
      601 tcggcttccg ggtccagttc cccgcgcggg tccgggtagc cggtgtctgc gggcaggctg
      661 agtgccagcc cccacaggct ggtctgcccc ggtcgccaga ccgtctttcc ttcactgtta
      721 aagagcatca gcgggcggcc cgtcaggtcg ctcaccgcat gattcgtccg cgtcacccac
      781 tgcggggcca cggacggatg cggctgccgt acccggctga actgctggct tatcagctca
      841 aagctgttaa acaccaggtg ccgtacgctg tacagtttat catcgcggta ttcccggatt
      901 tccgcaatac tgtcgccgtc ccacaggtac gtaaaacgga ttttcttccg gtcgcagcgt
      961 ttctctgttc gtctgccgct ggcgtcgtgg cggtattccc actgctcacc ctgtgcgctg
     1021 caatacccgg tcagctggtc ccggctgtcc caccggaacc gctctgtttc cgggcggtag
     1081 ccgtcacggt gttttgtccg gctgaccatc cggcctgcgg cgtcatagtc atactgcgtg
     1141 tttccggcct gtttcagccg gtggcctccg gcatactggt cagtctcttc acttatccgg
     1201 tgcctgcccg cagactgcgc cttcaggtag ccgcagctgt catagccata ggactcattg
     1261 ccttcgccat agcccagccc ggtgcctgag gctgtcaccg ccgtgacctg gccgttccgg
     1321 tccagccggt actcccgccg tgtttcccgt ccgtaatctt cccggctggt gacagccgac
     1381 acattacccg cgctgtcgta acggtactcc cgtaccagtg agggaaccag cccgccccat
     1441 gtggcttctt ccggaaaaaa tccggcgcgc tgactgacag gctgaccggc tgcattccag
     1501 ccctgttcac aggcaaaacc ccggttactg tgccggagca cttcacgtcc cgcctcatca
     1561 cggctgaacg tcagcgtctc atcccccggc aggataacct ctgccagttc tccggcggcg
     1621 ttgtaacggt aggtggtttt cagcagggat tcatcgggat gtctgtcgcg ttcatcttcc
     1681 ggtggcagga tacagcggat gacggtgtgt tcatcggggt agtggtattc aatggcccgt
     1741 cggccctgag tggtgacatt cacccgcccg cgttcgtcgt agccggtgcg ctgtgatgtg
     1801 ccgtccgggc tgaagatacc cgtcagcctg ccggatttgt cataggcata ctgcgttttg
     1861 ccttccggcg tgatgacttc tgccaggcgg cctgcggcgt cccggctgta gaggatggtt
     1921 tcctccaggc cgttgaccct ggcggtacac aggccatcgg cgtcatagcg gtaacgccag
     1981 accacgccgc cccagtcatg ctcttccgtc acccggccgc agttatccag aacataaaga
     2041 tatgactcac cctgcgggtt aatgacttct gtcagttgca gcgtgtcttt atcgtagcgg
     2101 tactgccagc ggtggccttc accgtccgtt cgcgccaccg gcaaatcaaa cgggccgtac
     2161 tccattgtcc agacaacacc gttaccgtcc gtgtacgcca gcaggttgcc gtggcggtca
     2221 tagcgaaatc cttccgcgcc ggtgccagtg cgttcaaggc cgctcagttt atggtgctgc
     2281 tgccaccgta accgcactga ctgaccgccg gggccgcgca tcttcaccgg caggtcgtga
     2341 ccgctgtatt cggtgtggtg ttcacggccc atcacgtcgg tgagtgtttc cggccggttg
     2401 tggtggtcgt actctatgcc cagacgccgt tcatcctggc ggaatattcc cgacagcagc
     2461 ccctgagcgg tgtatgccag ccgggtctct tcgccctgtg gcgacaccag acttaccggc
     2521 aggccgcgtt catcacactg ctgtgtccac gtctgcccgg cagggtcggt gatgttgctg
     2581 agcaggccgc gctcatcata gcggtactgc cactccgcgc cgtctgccgc cgtgtaacgg
     2641 cttaactgac cgtgtgcggc gttgtattca aatttctcca cccgcccggc aggggagtgg
     2701 cgggccagca ggtgatggaa ttcatcccat tcatacatgg tggtgctgcc gtccggcgct
     2761 tcttcccgca gaatattgcc gtcaggatca tagtaataac ggtgaatgcc cttgccgtcc
     2821 gtcacggtgg tgcagccgtc accatagtcc agcgtggcgt tgtaatagcc ctccgccccg
     2881 gtgacattca cgcaccggcc ctgttcatcg taggtgaaac ggctccacgt ctggtcgtta
     2941 tcggaccagc ggatgatccg gtccgcagcg tcgtactcat aaaacaggtg gtagtccagc
     3001 cgcgcatccg cttctgtcag ccgcccccgc gcatcctgtt cgtaggtcgc cagcgtctgt
     3061 atgccgttat ccgtgcggtg aatggctttc aggtagccgc tggcgtggta cgtcagttta
     3121 agccggatcc cgtcggtgtg actgacacct gtgatccggt actggctgtc gcgctcaaac
     3181 gtgatgcggt tgctggcggt gtcgctcatc ccgcccagca tgtacatccg gtcattgggg
     3241 ctgtgtgcca ggcggtcatc gctggtatcg tcagtcaccg ggtggcgcag acgcattccc
     3301 ggaaaggcgt cataaaagaa gcggctgctc agtgcatccc tgtcgaacag ctccagataa
     3361 tcatcgcggc ggcgcagaat gtactgggcg tgatacgggt taaccgccgt atcactgtca
     3421 ccgtcgaagg cgaagttcag cgtggctccc tggctgatga tatccacccg atttccctgt
     3481 tcacgcaccc aggcgtattc gctccaggag tcgctccagc ccacgccggt caggccatgc
     3541 agcggcagta cagaactgtg gaaacgggtg aaggagaggg ggagggtctg gcccagcgta
     3601 aagtcggtac gttcgtcgca gtacgcgccg gttaccggat cgacaggatc tgttacccat
     3661 ctgatgactt tattggcaat actttttaac gacttacctg tttttcccag caacgcgccg
     3721 agctttgccc ccagcagctt cccgcttttc cccagcaggt tccccagttt gccgagtctc
     3781 gccagattct tcagttttcc cagcagtccc gctggcggca gaagatagct ggctatcgtt
     3841 attcccccca ggatcattct ctgccacggc gggaactctg gctggatatc cagataggtc
     3901 acctgttcac cgccaataaa gacgtccggc gaaccttcca cgattgccgc gctgcactcc
     3961 agtttgtcgc ccttgcgcgc ggcgggctga ttgttgataa agacgttgct gctgccctgg
     4021 gctatcctcg gtggtttgtt ctccttgtcg cagagcgccg tgtcctcacc ggcttccggg
     4081 aaatccagat cttttttgat ttcgccacgc ccgcccacca tggtggcaaa ctccattaat
     4141 accggaccgg cgggaaacag aagcgatgcg ccggcgctga cgcgatccat aaaaccggca
     4201 ttgctgtccg tcagggtgat aacagcgttc ccccaggcct tcgccagtgg tactgctttt
     4261 tccgctgccg attcaagcag gtcagtggca atatcacccc atgagggttc cccctgtggg
     4321 acttcaggta ttgctccagg tgggggagga gggggaaggg tgacggcggc cctggcagcg
     4381 ggctttccgt taatgtttac atttttagaa ccagtattga tggccccgtc cttgtcacaa
     4441 aagctgttta tccatgactc aagctggttc tgtttctcct ccacataatc actcgcctta
     4501 tagctggcat acatcagcgc aggcaccaga accagcgcat aagggccggt ggcgcaggcg
     4561 gccataatca ggcaaccttc tgcgatggtc actatcgccc ccagcacggc ccctgccagt
     4621 gcccccagaa aacttttatg ctgaatttca tcgcccagcc tggcggcagg tgggccggac
     4681 ttctttgctg ccagcatctg tgcaatggct ttgtcgttgg ctgcccgttt catctggctg
     4741 gcgaccatcg cctgctgtga cagcattcgc attgccagcg taccgcctgc ggtcgctccc
     4801 tgtggcccgc ctggtccttc actcatgtgt tatccctcgt ttatgtcagc gcggctggtc
     4861 cttttggatg tcattctccg gtggcaacgg ggtgaagctc tttatcacct ccagcagcat
     4921 cgtcttttga ctgttcttca gggataagga ggtgctgaaa gtgaaggtca gcagcgtatc
     4981 tccacgccgc tggaatacgg ctatctggct aatccgcccc tgattcccct catcttccgg
     5041 accgttccag gaataatcca gcagccaggc cggggcctct gccagagtga tttcccggca
     5101 ggcatgttcg gtgtacccat caagtttgcc ctggtacagc gccagcgttt cctggtacag
     5161 ctctggtgtc agggttccct gtggaacggc ttcgcgggta atgaccaggt tgatgccact
     5221 gttatctgtg gacaccagga tctgcattga ctgatcctgc cattgagacg gcagttgaat
     5281 gtggccttca ttaaaagtga actgcataaa atactccctg ataatctgag tgaatcagtt
     5341 gatgaaaaca gaaccgccca cgatggtgat gccatccttc gacagcctga ttaccgtcga
     5401 ttccccgacc tgtagctgaa tgccatccgg cgtcagcgca attttgcttt cctccacggc
     5461 cagcaaaatt tccttaatcg ccgtataagc ctgtgtaccg tcgccttcgc caccattgtt
     5521 tacagcgtca acatgcaccg aaccggcaat cgtgcctctg tcctgaaaga catcctcaga
     5581 taaagaccct ttcagtactc cggtgatctt gtggcctttt tccaccgtaa gaaggcggtt
     5641 accttcctgg atcgtgaccg tctggtcatt ttttacggtt tcgcggtgat cgtgttttac
     5701 ggtggtggta cggtcgttga gcacttcggt atccatgttc ttctgcgcgt ggatgtagac
     5761 ctgttctctc cctgtcgcat catcaaactt cagctcatta aatccgctgc ccatgtacgt
     5821 tttggagcgg atggtcatct gcgtctttgt tcccggcagg ctgccggggg ggcggttttc
     5881 ctggtggtag gtgcgcccca taatgatcgg ctggtccgga tcgccgttga ggaagtctac
     5941 aatcacctcc tgacccacac gcggtatcgc caggtggcca aaaccggtgc ctgcccacgc
     6001 ctgtgccaca cggatccagc acgaactgtc ttggtctgcc gggttataac ggtcccagtt
     6061 gaacttcacc cgcacgcgcc catgttcgtc gcagaagatt tcctcgcctg ccggtcctgt
     6121 cacgacagcg ctctgcgggc cgtcgaccag cggtttcagc aacggctgtg gtcgccatgt
     6181 tctgtctgcc gggatcaccg caaaatggtt ctccagcgcc gtccccgctc cctgccgtcc
     6241 tggcaccgcc tgtggctgtt cgccgtgcag ttcacttgcc accacctgcc attcccggtt
     6301 caggttcgcc tgcggatgcc ccgtcagcac aattcgccgt cccggccata tctccggcga
     6361 gcggctcatt ccccgcgcgg tttctgcgtt gtttcgccag ccgtccatct gccagcgggc
     6421 aaagttctgc ccgtgggcgc tcttgaaacg tccggggtag tcatacactt catactgcgt
     6481 gcgctggtag tcctggtgct ggccttcctg ttcaaaacgt ccggcccagc cggggcgttt
     6541 aaaggtgtag tctttggtca ccacggaaga agggcggatt tgtgcgctgt agcggaactg
     6601 gctgatgcag agggtgctca cctcggtacg ggtgttcggg ttccatggga tttcaaaaga
     6661 ttcgggcaga tggcggactg tgtcgcacag caccaggctc tggtcggtac ttttgtaagc
     6721 atgctcctca taaaagaaga tgccttcctc cgccgccatc cggcacagga aatcgtaatc
     6781 agtctcaccg tactggacac aaaactcacg ggaaggatgc ggctcgctga acagcggact
     6841 ccattcggtc accccgtttt cctgcaacat cgtgccgagg atgcttttga tgtcctcgtt
     6901 ctggaagata cggaagttct ggcgcagacc ggcacgccac agcggcgggt gcaccttcat
     6961 gctgtacagc atctggtttt tgtcgttctc cccgagttca aaccaggtca ccacgccttt
     7021 cacccggcgc tgtacttcat cgccctgcca tatcgtcagg taggccattt tgtccagcac
     7081 ctgcgcaaat tcaagggaga gaaactgctg gctgaccagg gagagatcga gggaaaagag
     7141 cgaagagagt gactggttca gatgaaagga aaccaccgca aaagcatccg gcggcaggcc
     7201 gtccacttcc agtgtgaaac gtaatccggt tgacatagca acctccagta aaatgaataa
     7261 tgatccgtac cgtacaactg cggataatgt gataagcggg acgacttcag acgttattag
     7321 ggaaggaaca gaaaatgcta tggttatgtg agaattttgt ttaaatatta ctttacagaa
     7381 acgggtgtct gttcagtcaa aaaaataatc acaagctcaa cctcttgttt cttggcgtta
     7441 ttcaggacag gtacgcatat cacaacgagt aacaaaaacg caaagttaaa ccaatattca
     7501 taatatattt ttattcacag ca
//
LOCUS       DAZKHC020000168         7501 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZKHC020000168 DAZKHC020000000
VERSION     DAZKHC020000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.168"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..724)
                     /locus_tag="ACHOSM_RS22390"
                     /old_locus_tag="pgaptmp_004478"
     CDS             complement(<1..724)
                     /locus_tag="ACHOSM_RS22390"
                     /old_locus_tag="pgaptmp_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HGU4560836.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAISYFITGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVLADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLV"
     gene            890..943
                     /locus_tag="ACHOSM_RS22395"
                     /old_locus_tag="pgaptmp_004479"
     CDS             890..943
                     /locus_tag="ACHOSM_RS22395"
                     /old_locus_tag="pgaptmp_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560837.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(1042..1476)
                     /gene="uspA"
                     /locus_tag="ACHOSM_RS22400"
                     /old_locus_tag="pgaptmp_004480"
     CDS             complement(1042..1476)
                     /gene="uspA"
                     /locus_tag="ACHOSM_RS22400"
                     /old_locus_tag="pgaptmp_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HGU4560838.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            1867..2202
                     /gene="uspB"
                     /locus_tag="ACHOSM_RS22405"
                     /old_locus_tag="pgaptmp_004481"
     CDS             1867..2202
                     /gene="uspB"
                     /locus_tag="ACHOSM_RS22405"
                     /old_locus_tag="pgaptmp_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HGU4560839.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(2446..3945)
                     /gene="pitA"
                     /locus_tag="ACHOSM_RS22410"
                     /old_locus_tag="pgaptmp_004482"
     CDS             complement(2446..3945)
                     /gene="pitA"
                     /locus_tag="ACHOSM_RS22410"
                     /old_locus_tag="pgaptmp_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HGU4560840.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            4177..5379
                     /gene="yhiN"
                     /locus_tag="ACHOSM_RS22415"
                     /old_locus_tag="pgaptmp_004483"
     CDS             4177..5379
                     /gene="yhiN"
                     /locus_tag="ACHOSM_RS22415"
                     /old_locus_tag="pgaptmp_004483"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGU4560841.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(5694..6746)
                     /gene="yhiM"
                     /locus_tag="ACHOSM_RS22420"
                     /old_locus_tag="pgaptmp_004484"
     CDS             complement(5694..6746)
                     /gene="yhiM"
                     /locus_tag="ACHOSM_RS22420"
                     /old_locus_tag="pgaptmp_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HGU4560842.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNILFPIIGYTGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSANITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            7129..>7501
                     /locus_tag="ACHOSM_RS22425"
                     /old_locus_tag="pgaptmp_004485"
     CDS             7129..>7501
                     /locus_tag="ACHOSM_RS22425"
                     /old_locus_tag="pgaptmp_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560843.1"
                     /translation="MKVFHNIFKHISSSHQDKHSDQVNNHRHHGKVDKKHRSKIVEID
                     KLDKHSQIDNDLGLHIIYFLQHGRWKVNEHSSQIEKIWFYNSELSIDIQEYNSFADNT
                     TDTFIFTIIPDNNHVIKLSSPI"
BASE COUNT         2079 a         1784 c         1819 g         1819 t
ORIGIN      
        1 gcaccagatt ggcgacttct acgttgtgca tcagccatgc gcatacgaag atcatcacca
       61 cgctgccaag taatacgtac aacagcttgc tgaagctcat cggcttgaag tcgggttcag
      121 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
      181 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgagc acaggggcca
      241 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
      301 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
      361 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
      421 gcgacatgcc ggttatgaag tagctaatcg ccagcacaag tgctccgaga acaatggtgc
      481 gtttggtccc cagcaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
      541 gcgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
      601 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
      661 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
      721 tcatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
      781 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
      841 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
      901 tgttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
      961 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     1021 gaacacgtcg tggggggatg attattcttc ttcgtcgcgc agcggaacaa tcagcatatc
     1081 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatt agtttgctcc agaagtcctg
     1141 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     1201 gcccaggtcg ccgctgccgc tcagggtttc agtgattggg tagcctgcat tagtggaaag
     1261 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     1321 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     1381 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     1441 gagatcgacc gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     1501 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     1561 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     1621 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     1681 atatattgat agtggttaac cttctggaaa aaaaacaacc tgatctccta cactatctat
     1741 agagccgctc ggatgttggt caccacaaaa gcggtttctc caggacctgg tgttaactga
     1801 ttggcagcgt atcgggagcg gtagccccgg ggtggaactc cgcgggcagg tcgccgggga
     1861 ggaggtatga taagcacagt cgcattattt tgggctttat gtgtcgtttg cattgttaac
     1921 atggcgcgct atttctcatc actacgcgcg ttgttagtgg tactgcgtaa ctgcgatcca
     1981 ttgctctatc aatatgttga tggagggggc ttttttacct cacatggcca acccaacaaa
     2041 caggtgcgtc tcgtttggta tatctatgcc caacgttatc gcgatcatca cgatgatgaa
     2101 tttattcgcc gctgtgagcg ggtgcgtcgg cagtttattc tgaccagcgc attgtgtggt
     2161 ctggtggtgg tcagcctgat tgcattgatg atttggcatt aagcacttca gcaataataa
     2221 cgcataaaaa aagcgggcca gtaaactgac ccgctcgaga ctgattacaa acgcaaaatt
     2281 gcctgatgcg ctacgcttat caggcctaca taatctctgc aatctattga atttgcatgt
     2341 ttttgtaggc cggataaggc gttcacgccg catccggcat gaacaaagcg cactatgtca
     2401 caatctgaag cgggccagct gacccgctcg ttttggtgcg tacgattaca ggaactgcaa
     2461 ggagagccag tacagcccgc cggaaagcag taccgcagcc ggaagggtaa acacccaggc
     2521 catcagaatg ctggtcacgg ttttacgctg taagccgcca ccatctacca ccatcgtccc
     2581 cgcgacagaa gaggagagta cgtgagtagt ggaaaccggc atcccggtat aactcgccag
     2641 gccgatagac actgccgccg tcatctgggc agacatcccc tgagcgtagg tcatgccttt
     2701 cttaccgatt ttctcaccga tagtcgttgc cacacggcgc cagccaatca tcgtaccgat
     2761 acctaacgcc agcgcgaccg ccatgatgat ccacaccggt gcatactcga tggtgctaag
     2821 catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacgc caggcatctt
     2881 caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct ggctacgttg
     2941 atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt tcaggcggtt
     3001 gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg ttgcgccagc
     3061 ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg caggatgctg
     3121 ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt cgtagccagt
     3181 ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca acataaccag
     3241 accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga tagcggaaag
     3301 gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct tttcacgctc
     3361 cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc gacgcagcaa
     3421 gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca gagaaccgaa
     3481 aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg tcatcaacgc
     3541 attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg atgcaggtaa
     3601 accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag agaacaccat
     3661 ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca tatgcacaat
     3721 ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca ccgccgccat
     3781 aaccacggca agctgagaac gcatcgcgcg ggtatagata acggttgcca cggcgttggc
     3841 tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg caagcaataa
     3901 taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac gttacgccat
     3961 tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca aagtgaaata
     4021 tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt atcttatata
     4081 attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg aggaatcttt
     4141 acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga tgccattatt
     4201 ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggc aggacgccgg
     4261 gttctgctga tcgataatgg taaaaaacca gggcgcaaaa tccttatgtc tggcggtggg
     4321 cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca gaatccgcat
     4381 ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct ggtcaataaa
     4441 cacggcatcg catggcacga gaaaacgtta gggcaactct tctgcgatga ctccgcgcag
     4501 cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtgacctt cagattgcgt
     4561 agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct gaacggcatg
     4621 actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc ggggctgggc
     4681 gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct gccgacccgc
     4741 gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca ggtgctggcg
     4801 ggcgtggcgg tgccttccgt gattaccgct gaaaacggca tcgttttccg tgagaactta
     4861 ctcttcaccc atcgcggctt gtctggaccg gcggtgttgc agatttccag ctactggcaa
     4921 ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg atctcgaaac cttcctgaat
     4981 gagcagcgta acgcacatcc gaatcagagc ctgaaaaaca cactggcggt tcatctaccg
     5041 aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc gctaaaacag
     5101 ctcaacgtgc gtgaccaaca ggcactgatt agcacattga ccgactggcg cgtacaaccc
     5161 aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga caccaacgaa
     5221 ctctcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat cggcgaagtg
     5281 atggacgtca ccggctggct ggggggctat aacttccagt gggcgtggtc gagtgcgtgg
     5341 gcttgtgcgc aggatttgat tgcagcaaag tcgtcctgac ctatttccta aagatgaaaa
     5401 ttattagagt cgcattaaaa atgagctaat tttgatagtg gttatcttgt gattattttc
     5461 taatgagccc gtgaactgaa accctccatg cttaatataa ggtggatgga aaggtgattg
     5521 aaaactcacc cagtggccag atctttatga aatatgaaca ggttgaaaaa aacagtaact
     5581 ttgctgtttt ttatacttaa ccactatgca ttaatgctgc gttatttcat gatgcctaag
     5641 aaaaaccagc gttacgcaaa tggtcaacgc tggttttatc cggtacgttg caattatttt
     5701 ttagcagaac ccgcttctaa tattgaaacg attgagaaca acgtaaagca taccgctccc
     5761 aaaccgacca gaactcgcga aggaataaaa tatcccatgt cggtctgcgc catttccgca
     5821 agaaacgatg ccaggaaaag gcaaaacagg caggtgaaga cgggaatcat cggtattctg
     5881 ttggctaacg aacaggtacg tctccatacc agtgccagta gccagacttt tgagaatatg
     5941 ctgtaacaga tcattccgag gcaaataaga ataatgccgg gagccagtcg ggcgcttgca
     6001 tcggaactga ctaagacgta tatcccctgc agtaccgtga ttgagccgag aaaaataacc
     6061 caataacacc acagccagtg ttctttagtt gaaaacgtat tacgtgtttg atggacaatt
     6121 gtggcaacaa ggccaattag acaggcacag attgcggtta acccaagcaa tacgtgaccg
     6181 gcgacataat gcggagttat gttagcacta cgtaacagcg taatagacca aatgaaaccg
     6241 agcagggtga gtaaaacggg aacggcaatg aggcagttac cgattaatga agaataagcc
     6301 tgtaccggtg ttccgtcgct cttgctcccc gctgcatttt tgggaatgag cagaaagtga
     6361 ccggacgacg ctgccaccgt cgagacacat gcggcaatca taccaacgcc gaaaataaca
     6421 tggccggcga caaactcgtc tgccatcaca tcattgcctg ctaacagtgc ccaaccccat
     6481 atcatggtga taattgaccc tgtatagcca ataatgggga acaatatatt gtaaaacgta
     6541 ttaacgccgc gcgtgagctg cgaaatgata ataaatgcgg tagtgaataa tgccaggcat
     6601 atggctgcca gagaaatcag cacatgaccc gcgacgaaat actcgctttg ccctgaactt
     6661 tccagcacaa agccacctaa cgcgatacaa attaagccca taatcaaagg gattaatttg
     6721 aaaagccacc cgatatatat gttcacttta aactcctcag gtatccctgc taatattgaa
     6781 atgtgcatta atgataattt cagcctgtta atacaatgac gagatatcat taaagacaaa
     6841 tgcatagcat aactatagcg gggtttactt tacagagcgc tacttttcag cgttcaaaag
     6901 gccatgataa ataaatattt atcctaagaa taattcttat tctgttgttt ttttcaatta
     6961 tcttccaggg atggattgtt tttcattatt tagtaaaaga ccggatagtc tcggtgctta
     7021 aaatcatcgg atatataagt attattactt tgtatcgtcg taaatatgtc tttcagacat
     7081 gtatcgttta ttttgttact aacaagttat aaatattgcg ggttatttat gaaagtattt
     7141 cacaatatat ttaagcacat ctcttcaagc catcaagata agcattcaga tcaagttaac
     7201 aatcatcgac accatggcaa ggttgataaa aaacatcgtt caaagatagt tgaaattgat
     7261 aagctggata aacattcgca aatagataac gatttaggcc tgcatatcat ctatttcctg
     7321 caacatggtc gctggaaggt aaatgaacat agttctcaga tagaaaaaat atggttttac
     7381 aatagtgagc tatctataga tattcaagaa tataatagtt tcgctgataa tactactgac
     7441 acgtttatct ttacaattat accagacaat aaccatgtaa taaaattgtc gtctcccatt
     7501 a
//
LOCUS       DAZKHC020000169         7426 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZKHC020000169 DAZKHC020000000
VERSION     DAZKHC020000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.169"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..135
                     /locus_tag="ACHOSM_RS22430"
                     /old_locus_tag="pgaptmp_004486"
     CDS             <1..135
                     /locus_tag="ACHOSM_RS22430"
                     /old_locus_tag="pgaptmp_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560844.1"
                     /translation="KSNKAVKEYVEILKEFNICPEKVAEYLSEFSGKHFLDVYNYYSN
                     "
     gene            317..502
                     /locus_tag="ACHOSM_RS22435"
                     /old_locus_tag="pgaptmp_004487"
     CDS             317..502
                     /locus_tag="ACHOSM_RS22435"
                     /old_locus_tag="pgaptmp_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGU4560845.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRTIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            743..1732
                     /gene="nleB"
                     /locus_tag="ACHOSM_RS22440"
                     /old_locus_tag="pgaptmp_004488"
     CDS             743..1732
                     /gene="nleB"
                     /locus_tag="ACHOSM_RS22440"
                     /old_locus_tag="pgaptmp_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HGU4560846.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKIENERIIGVLVDGNFTYEQKK
                     EFLSLENEYQNIKIIYRADVDFSMYDKKLSDIYLENIHKQDSYPASERDNYLLGLLRE
                     ELKNIPEGKDSLIESYAEKREHTWFDFFRNLAMLKAGSLFTETGKTGCHNISPCSGCI
                     YLDADMIITDKLGVLYAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKS
                     KVDAHPYYDGLGKGIKRHFNYSSLHDYNAFCDFIEFKHENIIPNTSMYTSSSW"
     gene            1781..2455
                     /gene="nleE"
                     /locus_tag="ACHOSM_RS22445"
                     /old_locus_tag="pgaptmp_004489"
     CDS             1781..2455
                     /gene="nleE"
                     /locus_tag="ACHOSM_RS22445"
                     /old_locus_tag="pgaptmp_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HGU4560847.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRNDSPLGEQR
                     ANRFIELYNIKRDIMQELNYELPELKAVKSEMIIAREMGEIFSYMPGEIDSYMKYINN
                     KLSKIE"
     gene            2833..3471
                     /locus_tag="ACHOSM_RS22450"
                     /old_locus_tag="pgaptmp_004490"
                     /pseudo
     CDS             2833..3471
                     /locus_tag="ACHOSM_RS22450"
                     /old_locus_tag="pgaptmp_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3482..4183)
                     /locus_tag="ACHOSM_RS22455"
                     /old_locus_tag="pgaptmp_004491"
                     /pseudo
     CDS             complement(3482..4183)
                     /locus_tag="ACHOSM_RS22455"
                     /old_locus_tag="pgaptmp_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            4179..4256
                     /locus_tag="ACHOSM_RS22460"
                     /old_locus_tag="pgaptmp_004492"
                     /pseudo
     CDS             4179..4256
                     /locus_tag="ACHOSM_RS22460"
                     /old_locus_tag="pgaptmp_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            4273..4407
                     /locus_tag="ACHOSM_RS22465"
                     /old_locus_tag="pgaptmp_004493"
                     /pseudo
     CDS             4273..4407
                     /locus_tag="ACHOSM_RS22465"
                     /old_locus_tag="pgaptmp_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4992..5630)
                     /locus_tag="ACHOSM_RS22470"
                     /old_locus_tag="pgaptmp_004494"
     CDS             complement(4992..5630)
                     /locus_tag="ACHOSM_RS22470"
                     /old_locus_tag="pgaptmp_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGU4560848.1"
                     /translation="MPVVLNISDGRALPESELEALRNIARSNQSDTLIVGSRSLRLHH
                     VSFMDAFSVEPIQGSLLDRLGGRCRRLAESLERQLNHGNTFLQAFSLYMEQVRLTPFW
                     LEGGRNAVQTRINSHAFTVNPGDFPCCEQHLSCPITLCIPKTGVFVKNALHSKVCSLY
                     DKDALSEAIRHNVFHPLSREAFSPEMIVGREECYFDLTDQRFCIISGQDVRF"
     gene            5828..6412
                     /locus_tag="ACHOSM_RS22475"
                     /old_locus_tag="pgaptmp_004495"
     CDS             5828..6412
                     /locus_tag="ACHOSM_RS22475"
                     /old_locus_tag="pgaptmp_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bfpT-regulated chaperone"
                     /protein_id="HGU4560849.1"
                     /translation="MPANISGTPFNSFGISFIQKQSCWRKSDDILRCSMGQRTIKLST
                     NTLNNRILTSVARQSTKDINAWKRDERTVYPSRVINQGIDKYCAENSRNISSEVRQRV
                     FKLIEKDYSLKLNIIAAQSSINHLIIGNGRFGDKINMLCKGVSREVKNQTMDVIANQL
                     ADQFFQKHISPDVDIKQLRDDIPRYIMAASVISA"
     gene            complement(6736..7350)
                     /gene="espF"
                     /locus_tag="ACHOSM_RS22480"
                     /old_locus_tag="pgaptmp_004496"
                     /pseudo
     CDS             complement(6736..7350)
                     /gene="espF"
                     /locus_tag="ACHOSM_RS22480"
                     /old_locus_tag="pgaptmp_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
BASE COUNT         2318 a         1572 c         1517 g         2019 t
ORIGIN      
        1 aagtcaaata aggcagtaaa ggaatatgta gaaattttaa aagaattcaa tatatgccca
       61 gaaaaggtag cagaatacct aagcgagttc agcggaaaac atttcctcga tgtatataat
      121 tattattcca attgaaaaaa atataaagta tctatcacgg cactctcagt tgagtatcac
      181 aggcacatgt attttagccc cctgagtcta cagacaacca atattactta aataagtgat
      241 aatcttaatg ctgtttttta gaacagatga ttgatatttt tgggccggag aaacgcagac
      301 aggctttgtt gaataaatcg agcttttgct gagtttaagg atcagatcac tcattttcct
      361 gcaaaagctc attgggcgaa acaatctgac tcagagaacc cggattaaac gtctggcccg
      421 cagaaccatc tatttctcac gcactgtagt acgtaataaa aaagtcatcg gtacttttat
      481 caaaaaacat atattctatt aattattgcc atcacctttt tcttattgac aacattactc
      541 tgctgattgt taaaatctaa cctgtattca gggttacgat tctatacaat agaacaggga
      601 ggggaaagga tgtcatcgtt aatgagactt tttgttaatg tcaacaatgc tatgaattta
      661 ttttagaaaa acaattaagc tgacgtagtg ttccgtctaa atgacggggc gatattaaca
      721 tgattagaac aagaggaatt ttatgttatc ttcattaaat gtccttcaat ccagcttcag
      781 aggaaagaca gccttatcaa atagtacact tctccagaaa gtttcttttg ctggaaaaga
      841 atatcctctg gaacctattg atgaaaaaac ccctattctt tttcagtggt ttgaagcaag
      901 gccagagcga tacgaaaaag gagaagtacc aatattgaat accaaagaac atccgtattt
      961 gagcaatatt ataaatgctg caaaaataga aaatgagcgt ataatcggtg tgctggtaga
     1021 tggaaatttt acttatgaac aaaaaaagga atttctcagt cttgaaaatg aatatcaaaa
     1081 tataaaaata atctaccgag cagatgtgga tttcagcatg tatgataaaa aactatctga
     1141 tatttacctt gaaaatatcc ataaacaaga ttcataccct gccagtgaga gggataatta
     1201 tctgttaggc ttattaagag aagagttaaa aaatatccca gaaggtaagg actctttgat
     1261 tgagtcatat gcagaaaaaa gagaacatac ttggtttgat tttttcagga atttggccat
     1321 gttgaaggct ggaagtttgt ttacagagac gggaaaaact ggatgccata acatatcgcc
     1381 ctgtagcgga tgtatatatc ttgatgccga catgattatt accgataaat taggagtcct
     1441 gtatgctcct gatggtatcg ctgtgcatgt agattgtaat gatgagataa aaagtcttga
     1501 aaatggtgcg atagttgtca atcgtagtaa tcatccagca ttacttgcag gcctcgatat
     1561 tatgaagagt aaagttgacg ctcatccata ttatgatggt ctaggaaagg gtatcaagcg
     1621 gcattttaac tattcatcgt tacacgatta taatgctttt tgtgatttta ttgaatttaa
     1681 gcatgaaaat attataccga ataccagtat gtataccagc agttcatggt aagtcttccg
     1741 gttgtggttg aaaaatatcg ttctttaggt atagattcag atgattaatc ctgttactaa
     1801 tactcagggt gtgtccccta taaatactaa atatgctgaa catgtggtga aaaatattta
     1861 cccggaaatt aaacatgatt actttaatga atcacccaat atatatgata agaagtatat
     1921 atccggtata accagaggag tagctgaact aaaacaggaa gaatttgtta acgagaaagc
     1981 cagacggttt tcttatatga agactatgta ttctgtatgt ccagaagcgt ttgaacctat
     2041 ttccagaaat gaagccagta caccggaagg aagctggcta acagttatat ccggaaaacg
     2101 cccaatgggg cagttttctg tagatagttt atacaatcct gatttacatg cattatgtga
     2161 gcttccggac atttgttgta agatcttccc taaagaaaat aatgattttt tatacatagt
     2221 tgttgtgtac agaaatgaca gccctctagg agaacaacgg gcaaatagat ttatagaatt
     2281 atataatata aaaagagata tcatgcagga attaaattat gagttaccag agttaaaggc
     2341 agtaaaatct gaaatgatta tcgcacgtga aatgggagaa atctttagct acatgcctgg
     2401 ggaaatagac agttatatga aatacataaa taataaactt tctaaaattg agtagagaag
     2461 ctcagccact tgtgtagggg ggctaaaatt aattctattt cattatatga gaacaccaat
     2521 aagaataata atatttgatc acaactgaag atgcttttta caatgaagaa ttattgttaa
     2581 aaaacgcttt gttgaatcaa ccgaactgtt gctgagttgc aggatcacat cacgtatttt
     2641 cctgaaaacg caggctgctt agtagcaaag cagaagttca aaatcaccaa ctaaatagct
     2701 gcgccgaata gtagatcaat gaaagggaac tcagcccgga ttgtgcgatc tgatcaatcg
     2761 ccaaaaatcc ccccctaacc ggactgagcg atgccgatca tagcaccaat tccccgcgac
     2821 gaatgacgcc tgatgcaaaa agcaatctat aaaactcacg ataaaaatta tgcccgcagg
     2881 ctgaccgcca tgctgatgct gcaccggagc gctcgcgtca gtgacgttgc cagaacgctc
     2941 tgctgcgccc gttcctccgt caggcgctgg attaactggt tcacgctgtc gagtgttgaa
     3001 ggtctgaaat cattacccgc aggacgttcc cgccgctggc cgtttgagca tatctgcaca
     3061 ttgtttcgtg aactgataaa acactctccc ggatattttg gctatcagcg ttcacgctgg
     3121 agtacagaac tgctggcaat aaaaatcaac gagataaccg agtgccagtt acatgccgga
     3181 accgttcgcc gctggttgcc attagtcggg cttgtgtggc gcagggctgc gccaaccctg
     3241 cgtacccgta acccgcataa agcagcaatt cacaaagcac tggacgaatg tagtgcggag
     3301 catccggtct tttatgaaga tgaagtgtat atctatctta atcccaaaac cggtgcggac
     3361 tggcaacttc gcagacagca aaaacatgtg gtaacaccgg ggcagaatga aaaatactat
     3421 ctggctggtg ctttacatag tgtaacgggc aaagtcagct atgtgggtgg tggatttgcc
     3481 cctatatttc cagacacctg ttatcactta acccattact ggcttgctgc cgtagatatt
     3541 cccgtggcga gcgataaccc agtgcactat gcggatgcca ttcgttataa tgctcgaacg
     3601 cctctgcaag gttctttgct gccgttaacc cgtctggttt gggcatgaca ctgatgtagt
     3661 cacgctttat cgttttcacg aagctctctg ctatgccgtt actctccgga ctccgcaccg
     3721 ccgtgctctt cggttcaagc cccaacatcc gggcaaactg ccgtgtttca ttagcccggt
     3781 agcatgaacc attatccgtc agccactcta ctggagacgc cggaagctcg ttgccgaagc
     3841 ggcgttccac cgctcccagc atgacgtcct gtactgtttc actgttgaag ccgcccgtag
     3901 tgaccgccca gtgcagtgcc tcacggtcac agcagtccag cgcgaacgtg actcgcagtt
     3961 tttctccgtt atcacagcgg aactcgaacc cgtcagagca ccatcgctga ttactttctt
     4021 tcacagccac tctgcctgta tgtgcccgtt tcgatggcgg tacagcaggt tttcgctcaa
     4081 gcaacagcgc attctggcgc atgatccggt aaacacgttt ggcattgatc gcaggcatac
     4141 catcaagttc tgcctgtctg cgaagcagcg cccatacccg acgttatggc gtcagtctca
     4201 ttcatctgga tgccagcgct gaatatctgc cgggcctgcc cactttcgac tggtaatccg
     4261 gaaattcaca acatgaaatc attaaccacg gaaacagtgc tggatattct gattacatgg
     4321 ctgcaggaca atatcgactg cgaatcagga attatcttcg acaacgatga ggataaaaca
     4381 gattcggcag cactgttgcc ctgtgtcggc ggcagttcac tgactttcag attctcacaa
     4441 ttaataacgg acaggctttt tatattgacc ggtagccctg taagaccggt cagcttatta
     4501 cagttctcta ttgttatcgc tgtgatttct tccggtaaat gaggcaagaa ctccatgtca
     4561 caatttttta tatgcagaca accactttcc cgtgcatctt ccagttgccg ggacgtaatt
     4621 tcacttttaa agcttgcagg tgtttcagta atatgatgac aggcagtccc tgacaatttt
     4681 aattgtgatg tggaaagcat caataaattc cattttcaga aaataaatca ccataaggta
     4741 agccccatcc gaggagctta cctgacaaat ttcatacaga gagcctgtat ccagggtttc
     4801 ccgatggcga aaaggcatca ttacagacgc tatttcacgc tggaaaagca agtacagtta
     4861 ctacatgttc attccagaat aaacccagca atctgacccg gattctaaca accagcaaaa
     4921 taatgttgtc aatcgaagac gaataaaaaa gcagcagtca gtacaactca gtctgtatta
     4981 atatccccca ctcaaaaacg tacatcctgc ccggaaatga tgcagaaacg ctgatcagtt
     5041 agatcaaaat aacactcctc tctgccaacg atcatttctg gagaaaacgc ttcacgactg
     5101 aggggatgaa aaacattgtg tcggatagcc tcagacaagg catccttatc gtataaagaa
     5161 cagacttttg aatgcagtgc atttttaaca aaaacgcctg tttttggtat acataatgtt
     5221 atcgggcaac tcagatgttg ctcacagcaa ggaaaatctc caggattgac ggtaaatgca
     5281 tgagaattaa ttctggtctg cactgcattt ctgccccctt ccaaccagaa tggggttaaa
     5341 cgaacctgct ccatatataa actgaacgcc tgcaggaaag tattgccatg attaagctgt
     5401 ctctccagac tctctgcaag gcgacgacac ctccctccaa gacggtcaag aagactgccc
     5461 tgaatcggct ccacactaaa agcatccata aatgaaacat ggtgcagcct cagactacga
     5521 cttcccacaa tcagagtatc actctgattg cttcttgcta tattacgcag agcttccagt
     5581 tcactttcag gcagcgccct tccatctgag atatttaata cgacgggcat attatctccc
     5641 tctgtaatgt atttagctta tccaggccaa caacaaataa acatccatac ataaaataaa
     5701 acagacaaaa cattttggca acataccatc aatcattgac ttatcattat ttacaccaaa
     5761 gcgttgcgat actaaaataa ttataaactt gctacattgt aaatatagac aaataaaagg
     5821 aattactatg ccagcaaata tatcaggaac acccttcaac tcatttggta taagctttat
     5881 ccagaaacag tcctgctgga ggaaatctga tgatatcctg cgatgttcaa tgggtcagag
     5941 gactatcaag ttatccacta atacgctgaa caaccgaatt ttgacctccg tcgcccgaca
     6001 aagtaccaaa gatattaatg cctggaaaag agatgagcga acagtttacc catctcgcgt
     6061 gatcaaccag ggaattgaca aatattgtgc tgaaaatagc agaaacattt ccagtgaagt
     6121 acggcaaaga gtttttaaac tcattgaaaa ggattacagt ctcaagttga atatcatagc
     6181 agcgcaaagc tctatcaacc atctcataat aggaaatggt cgtttcggtg ataaaataaa
     6241 tatgctttgc aagggagtga gtagagaagt aaagaaccag acaatggatg taatagccaa
     6301 tcaactcgca gaccagttct tccaaaagca tattagtcca gatgttgaca tcaaacagct
     6361 acgagatgac atccccagat atattatggc tgccagcgtc atatcggcat aaatagagat
     6421 atcccttaac gctaaccaga aaaaaaatac ctataagagc ctgtacatag agttgtgtcg
     6481 ttgcctgatg ttgatatgtc aaatccaaca tcaaatagcg gtaaatttat ggataaaaaa
     6541 ttacaggctc tggttaacga aacacccccc tcttgctcta ttcccctctt tcatcattta
     6601 ttacaatatt tatttcaata aaaaccgata ttaccaatca catttatcac aatgcaactt
     6661 aatatcggtt tttatctttt aactctctaa acaatcaaca gtaattaata attttaaatc
     6721 tactttttat gcctttttcg acagttcata ggcagctaaa tgatctttta atgcctgagc
     6781 aataggcggt aatggccggg atgcctcgga tgcctgtcca cttgtcggtg gcggcggtgc
     6841 cgggcggctt ggcttaagac ctgaagtatc aagacttttc gatttttcat aggcagccaa
     6901 atgatctttt agcgcctgag caatgggcgg taaagaccgg gatgacccag ataccaaccc
     6961 gcttgtcggc ggtggcggtg ccgggcggct tggcttaagg cttgaagtat caagactttt
     7021 cgacttttca taggcagcta aatgatcttt taatgcctga gcaatgggcg gtaaagggcg
     7081 ggatgccccg gaagcctgtc cacttgtcgg cggcggtggg gccggacgag agggagtaaa
     7141 tgaagtcgcc gggctactca cattaaaagt cgttctcgca ttgatattcg acgtaccggg
     7201 agaaaaagga gattgagccc taatcggatt cagacgaaca gcctgagggg acactgaaaa
     7261 gccagataac cccgcagagc ttacccggct tgccgcacta gtaatatgcc gtcctagtgt
     7321 agaaacagct tgactaattc cattaagcat aattcatgcc tttttatatc taattaacag
     7381 gaataattat taatttatat taaaattaaa aatagttaac gactat
//
LOCUS       DAZKHC020000170         7397 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZKHC020000170 DAZKHC020000000
VERSION     DAZKHC020000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.170"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(156..518)
                     /locus_tag="ACHOSM_RS22485"
                     /old_locus_tag="pgaptmp_004497"
     CDS             complement(156..518)
                     /locus_tag="ACHOSM_RS22485"
                     /old_locus_tag="pgaptmp_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HGU4560850.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            803..1012
                     /gene="yibT"
                     /locus_tag="ACHOSM_RS22490"
                     /old_locus_tag="pgaptmp_004498"
     CDS             803..1012
                     /gene="yibT"
                     /locus_tag="ACHOSM_RS22490"
                     /old_locus_tag="pgaptmp_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HGU4560851.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            complement(1024..1611)
                     /gene="mtlR"
                     /locus_tag="ACHOSM_RS22495"
                     /old_locus_tag="pgaptmp_004499"
     CDS             complement(1024..1611)
                     /gene="mtlR"
                     /locus_tag="ACHOSM_RS22495"
                     /old_locus_tag="pgaptmp_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HGU4560852.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(1611..2759)
                     /gene="mtlD"
                     /locus_tag="ACHOSM_RS22500"
                     /old_locus_tag="pgaptmp_004500"
     CDS             complement(1611..2759)
                     /gene="mtlD"
                     /locus_tag="ACHOSM_RS22500"
                     /old_locus_tag="pgaptmp_004500"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU4560853.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            complement(2989..4902)
                     /gene="mtlA"
                     /locus_tag="ACHOSM_RS22505"
                     /old_locus_tag="pgaptmp_004501"
     CDS             complement(2989..4902)
                     /gene="mtlA"
                     /locus_tag="ACHOSM_RS22505"
                     /old_locus_tag="pgaptmp_004501"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HGU4560854.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVETLSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            5439..5801
                     /locus_tag="ACHOSM_RS22510"
                     /old_locus_tag="pgaptmp_004502"
     CDS             5439..5801
                     /locus_tag="ACHOSM_RS22510"
                     /old_locus_tag="pgaptmp_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU4560855.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5804..6940
                     /locus_tag="ACHOSM_RS22515"
                     /old_locus_tag="pgaptmp_004503"
     CDS             5804..6940
                     /locus_tag="ACHOSM_RS22515"
                     /old_locus_tag="pgaptmp_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4560856.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT         1802 a         1852 c         1842 g         1901 t
ORIGIN      
        1 taaaaatgca ttttcagaat atcaaccagc ggtaaatcga cgtaaaggga gtgaattgtt
       61 tggtaattag gcgagtagac aggagagtcc ccgcattgac cagctaaggc aagccagatg
      121 cgggttgata tgatcgaacc agtcgtctga ggcgcttaaa acgcagtctt agagaatcca
      181 gtcatctcct gcaggcccat ttcacggccc agcgcggtca ttgggtgcac aaccaccagt
      241 ccgcgaacac ttttcttcag cttgcccata tcagcctgct cttttttggt aattgcgcgc
      301 tgaaacggca tcttcatcag cttctgtgct tctttactca gtttttggct atgaacttca
      361 cgcaggcgag caatttctgc ttccagcgtg gctttctctt tttccagctc ggcatatttg
      421 tctgcggctt caaccagcga cagatcggcc tgctggtggc ggatggcgtc cagacgatcg
      481 ctgagacgtt tgatttcgtt tttttcgact tctttcatgt gatggcttct gataaagata
      541 aatttatagc ccctagtatg cgcgaccagg tgctttgttc acaactgatg acattgcaat
      601 agttctgtaa cgtaatcatt aacatgctta aaaactacgc aatttattga ttaattaaga
      661 aatctataga aaaaagataa taaacagcag cggatgatta ttgaggattc agcacgtttt
      721 ccttaatgtc atctttaccg ggaacctaaa ctaaaattgg cggccgcgca ttcccggcgc
      781 ggggtaatac ggagatatca tcatgggcaa attaggtgaa aacgttccgc ttcttatcga
      841 taaagccgta gatttcatgg catcaagcca ggcgttccgg gagtatctga aaaaacttcc
      901 tccccgtaac gcgattccgt ccggaatacc cgatgaaagc gtgccgttat atctacaacg
      961 tctggagtat tatcgtcggc tttatcggcc gaagcaggta gaggggcagt aagcagacgt
     1021 tgcttacttt tgaaaggctt tttttaagct gattttggaa atcagctcag tcagtgaaag
     1081 gaccattgtt gatcgcacag cctgttgata gcgctgaatt tgcattgcat acaaactgga
     1141 gtctgctggt tcaaactgtg gcggcggtgg taacgccgcc acgcagtgca gttcaccaaa
     1201 gggtccaagg atttcgtcgt cggtaaaggc gtactcgttg ccgtcgtgat ttagctcttc
     1261 acgcaatgcc atcagcagtt ccgcatcttc gtattcctgg cggttaatga cgcccaaccc
     1321 gtaaatgagt tttaaacgca cagaaagatc gcccagcgga ccgtcgccgt cgagtaacgg
     1381 ttctacagca tacttcaccg cgtaatcgtc tttgcggaat acctgaagca ccagaagatt
     1441 taccgcctcg gtcaaaagct cgacggcggt gatcagaaag cttcgcacgg ttttgccagc
     1501 attcagacgc tcaagcacac ggttttcaaa ggcctgggtt tgttccattg ttgcctgcat
     1561 atctgacaat ctgttattcg ggcgcagggt gtcctgcgcc tggtccacca ttattgcatt
     1621 gctttataag cggttaccgc ctcggataca acctcgctgt tggcatcaag atcggaaatc
     1681 tgtgccagcg ccgcctgcgg acctttgtca gcgatcagtg ctgccagttc ctgagcctgc
     1741 ggatcatctt cactgcggaa gtgcattgca ccagcaatcc cctgaatcag attcttgtgc
     1801 ggaaggctgt attccagcgt accgagcaat ggcttgatca gacggtcgcc agcactcagt
     1861 ttacgcagcg gctgacggcc tacgcgctct acatcatctt tcagatacgg gttctcaaaa
     1921 cgaccgagga ttttctggat gtacgccgca tgtttgtctg cgtcaaagcc gtagcgcttg
     1981 atcagtaccg caccactttc ttccatcgca ccttttacca ccgcgcggat tttctcgtcg
     2041 agaatagcgt cacgaatggt ctgatgaccg gccagttttc cgaggtacgc ggttatagca
     2101 tgacccgtgt tcagggtgaa gagtttacgt tcgacaaatg ccatcaggtt gtcggttaac
     2161 tccatgcctg ggatgttcgg cagtgcgcct ttgaactgcg ttttatcgac aatccattcg
     2221 ctgaaagttt ctaccgtcac ttccagcgga tcgttagttg ccgaagccga aggcggtacg
     2281 atgcggtcaa cggcggaatc gacaaagcca acgtgttctt ctacccacgc tttggcgtct
     2341 tccggcaggg cgttcatcac atggcctttc agctgcgtgg taccgcgtac catgttttca
     2401 caggcgatga tgttcagcgg ggattcatta ccttgttctt tgcgtttcac cagccctttg
     2461 gcgatagccg gagcaatacg ttccagcaca accgggccaa cggcggtagt gactaaatcg
     2521 acctgagcaa tcagatcaac gacatcatca ccaatgctgc tgacagcatt gacgccggaa
     2581 acggtatcta cctgctcggt ttcaccaacc acatgaacct gatagctatg acgggcattc
     2641 agggcatcaa gtaccacctg attgacatcg gcaaacgtca gttggatacc cgcgtctgcc
     2701 agcagtttac cgataaagcc acgaccgata ttacctgcgc caaaatgtaa tgctttcata
     2761 gtattaacct tcatcaatgt ttttacccga gagggctggg gtgaggcttt tccctcaccc
     2821 aaaccctctc cgtgaggaga ggggcagcgc cggatgcggc gtaagcgcct tatccggcct
     2881 acaagaacgt gcaaattcaa caaatcgtga tgcatcctgt aggcctgata agcgtagcgc
     2941 atcaggcaat taaccccacc ttctccatgt ggagagggtg ggattggatt acttacgacc
     3001 tgccaacagt tccagcactt catccacgct ggtggtgtgt gccagacgct cgatgacgga
     3061 ctcatcatcc agtgcattgg tcaggctggt gataacctga atgtgctcgt tgttacgggc
     3121 tgcaatacca atcaccagac gggcaatgtc atcttcttct tcaccgaagc gcacgccttc
     3181 cgggtactgg cagaacacca cgcccgtttt cagtacgcga tctttcgctt caaccgtacc
     3241 gtgtggcacc gcgatagact cacccagata agtcggggtc agtttttcac gatccagcat
     3301 cgcctgaacg tattccggct caacgtaacc gcctttcacc agctgctcgc cagcaaaacg
     3361 aatcgcttct tctttggttg ctgctttgcg accgaggaag atgttctccg cgcccagttt
     3421 gaacaggtta gcactggaat cgtcaaagct gtctttcaga ctgtctttca ctttcacttc
     3481 gttttcagta tggcgctggg cagcaacgag acgttcggtt aggctggtgt acaggccgct
     3541 gtcgaggaag ttggtcagcg aaatatgctg tgcctgcgga acctggcgca tagcgcgttc
     3601 ggtcaggtca cggtgagtga tgacgaggtc cacatctggc ggcaggttgt tgatcgcgct
     3661 gttagtgacc gaaatctgcg acagacctgc atcctgaatt ttcttacgca gcacgcctgc
     3721 gcccatcgca cttgaaccca taccggcgtc acaggcaacg atgattttac gtacgtggct
     3781 caggtcgtta gtcacatcgc ccgcagacag cggagatgtg cctttagact cagctttcat
     3841 gtcctgcata cgacgagttg ctgcttcaat atcatcttct tctttcactt tgctggtttt
     3901 cagcaaaata gcagagacaa cgaaggagac agccatcgcc gcacacacac ccgcgatgtt
     3961 agcgaaataa gcaccttttg gtgtcatcgc cagtacagcc aggatagaac ccggagatgc
     4021 cggagaaacc agaccaccgc ccaggatagt cagcgtgaac acgccagtca taccgccgag
     4081 gatgactgcg aggatcagac gcggattcat cagcacatac gggaagtaga tttcgtggat
     4141 accacccagg aagtggatga ttgccgcacc gcccgcagac tgtttagcgc taccacgacc
     4201 aaagaacatg tacgccagca gcacgcccat acctggacct gggttagctt caatcaggaa
     4261 gaagattgat ttacccagtt catgggactg ctgaataccc agcggcgaga agataccgtg
     4321 gttaatggcg ttgttgagga acaggatttt cgccggttca acaaagatag acgccagcgg
     4381 cagcatgtca tggacaacca tgaagttaac gcccgcagcc agcattttgg acagggtttc
     4441 aacaatcggg ccaatgccga ggaatgccag aatagcgagg atcatcccga tgatgcctgc
     4501 ggagaagtta ttcaccagca tctcaaaacc ggatttgatc ttaccgtcta cccagcggtc
     4561 gaagtgctta atgcaccagc cgcccagcgg acctgcaatc atagaaccga ggaacatcgg
     4621 catgtctgcg ccgacgataa cgcccatggt ggtgatggca ccgactacgc cgccacgttc
     4681 gccgcctacc agcttaccac cggtataacc gatcagcagc ggcaggagat aagtgatcat
     4741 cggcccgacc agcttcgcca gcgtctcgtt cggtaaccac cctgttggaa taaataacgc
     4801 ggtgatgata ccccacgcga taaacgcgcc gatatttggc atcaccatgt tgctgaggaa
     4861 acgaccaaag ctttgcactt tgatcttaat atcggatgac ataaaaatac cccttcttat
     4921 gtttgctgtc gcgcaggctg gaagcccgag gttttttgtt aatgtggcgg cagaggtagc
     4981 cggaccttgt agatactgcg aaatctggca ctgaatcggt taactgtcca gtcgacggcc
     5041 ttttgtgtga tatcaatcac ataaatatag ggggtagaga ggtaattgac gtgacgttca
     5101 tcacaaaacg cttgtgtaaa aaagcaacaa ggcggcgatt ttcgctaaaa atggcattta
     5161 aatgtgagtg gtgtcacatt tttgttttga tggtttgttg aatctttgtg atctgaatca
     5221 caagatattt tcttacggcg ttcacctgat gtgatctaca gcatgttatg ccttcgtcat
     5281 acaatcgtta tgtaactaag caactcatca cctctaaaaa ttattctttg gtgttatggc
     5341 gagaatcgcg aacccggatt attgtcaaaa tatcagcctg actcccttct ggctgccagc
     5401 ccaaaaacaa cactgataaa cgtgaatccg aggcagatat gtttctaaac tatttcgcgt
     5461 tgggagtgct gatcttcgtc tttctggtga ttttttatgg aatcatcgcg atacatgaca
     5521 tcccttatct gattgccaaa aagcgcaatc acccccatgc cgacgctatt catacggcgg
     5581 gctgggtgag cctgtttact ctgcatgtta tctggccgtt tctgtggatc tgggcgacgc
     5641 tctatcaacc ggagcgtggc tggggtatgc agtcacatgt tgcgtcgcag gagaaagcga
     5701 ctgacccgga aatcgcctca ctttctgacc gaatttcccg gctggagcat caactcgccg
     5761 ccgagaaaaa gactgactat tccacgttcc cggagatcta attatggatc tattgattgt
     5821 tttaacttac gtggcgctgg cgtgggcggt ctttaaaatc ttccgcattc cggtaaatca
     5881 gtggacgctg gcgacggcgg cgctgggagg cgtgtttctg gtgagtggtt tgattttgtt
     5941 gatgaactac aaccaccctt acacttttac cgcgcaaaag gcagtgatag cgatccctat
     6001 cacgccacag gtgacgggaa ttgttactga agtcactgac aagaataatc agcttattca
     6061 aaagggcgag gtgcttttta agctcgaccc ggttcgttac caggcgcgag ttgacagact
     6121 tcaggctgac ctgatgacgg cgacgcataa tataaagacg ctgcgtgcgc agctcactga
     6181 agcgcaggcc aacaccaccc aggtttcagc ggagcgcgac cgtctgttta aaaattatca
     6241 acgttacttg aaaggcagcc aggcggcggt gaatccgttc tcggaacgtg acatcgacga
     6301 tgcgcggcaa aatttcctcg cgcaggatgc gctggtgaaa ggctcggtgg cggagcaggc
     6361 gcagatccag agccagctcg acagtatggt taacggcgag caatcgcaga ttgtgagctt
     6421 aagagcgcaa cttactgaag caaaatataa ccttgagcag actgtcattc gcgcgccgag
     6481 caatggctac gttactcagg tactgatccg cccaggtaca tacgcagctg ccttgccgct
     6541 gcgtccggtg atggtcttca tccccgagca aaaacggcaa attgtcgccc aatttcggca
     6601 aaactcgctg ttacgtctga aacccggcga tgatgcggaa gtggtgttta acgcgctacc
     6661 tgggcaggtg tttcacggca aactgactag tattttacct gtcgtgccag gcggttctta
     6721 tcaggcgcag ggggtattgc aatcattaac ggtcgtgccc ggcacggacg gtgtgctggg
     6781 aaccattgaa ctggacccta acgatgatat cgatgcctta cccgacggca tctacgccca
     6841 ggtggcggtt tactccgacc atttcagcca tgtttcggtg atgcggaaag tgctgctaag
     6901 aatgaccagc tggatgcatt atctttattt ggatcattga gggggagggg ggagattatg
     6961 gaactttgct ccttgtaagg actaattatt tttgcatatt acttcattag ctgcgatgat
     7021 ttttctttaa tattcattat gttaataagt tctggatgag tgtgctctca ttggattttt
     7081 tatccgtctt gttctgccgc aggatgagac tatacatcgt cgagatcgca atgatgatat
     7141 gagttgttcc tcctgcgacg gctctaatca aaaaagagca ggagtgcttt tccatctatt
     7201 gcgatgatgg ctttattatc tgaaggatga cagtcacatt taaaaaatat ttaatctata
     7261 gatcacaatg tagggtcatg gaatatatgt atacaactat tttcttcttc acttttaatt
     7321 atcgtattta ttataaaact aagttaaaaa aactcctgga gagtgagtta ttaatatatt
     7381 tttaaataaa attaagt
//
LOCUS       DAZKHC020000171         7098 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZKHC020000171 DAZKHC020000000
VERSION     DAZKHC020000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.171"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            73..1377
                     /gene="adeQ"
                     /locus_tag="ACHOSM_RS22520"
                     /old_locus_tag="pgaptmp_004504"
                     /gene_synonym="yicO"
     CDS             73..1377
                     /gene="adeQ"
                     /locus_tag="ACHOSM_RS22520"
                     /old_locus_tag="pgaptmp_004504"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HGU4560857.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDGN
                     GKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLV
                     MFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSIT
                     EGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1430..1882
                     /gene="yicN"
                     /locus_tag="ACHOSM_RS22525"
                     /old_locus_tag="pgaptmp_004505"
     CDS             1430..1882
                     /gene="yicN"
                     /locus_tag="ACHOSM_RS22525"
                     /old_locus_tag="pgaptmp_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HGU4560858.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2093..3283
                     /gene="nepI"
                     /locus_tag="ACHOSM_RS22530"
                     /old_locus_tag="pgaptmp_004506"
     CDS             2093..3283
                     /gene="nepI"
                     /locus_tag="ACHOSM_RS22530"
                     /old_locus_tag="pgaptmp_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HGU4560859.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3324..3617)
                     /gene="yicS"
                     /locus_tag="ACHOSM_RS22535"
                     /old_locus_tag="pgaptmp_004507"
     CDS             complement(3324..3617)
                     /gene="yicS"
                     /locus_tag="ACHOSM_RS22535"
                     /old_locus_tag="pgaptmp_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HGU4560860.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3839..4657
                     /gene="nlpA"
                     /locus_tag="ACHOSM_RS22540"
                     /old_locus_tag="pgaptmp_004508"
     CDS             3839..4657
                     /gene="nlpA"
                     /locus_tag="ACHOSM_RS22540"
                     /old_locus_tag="pgaptmp_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HGU4560861.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKITTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            complement(4661..5584)
                     /gene="yicL"
                     /locus_tag="ACHOSM_RS22545"
                     /old_locus_tag="pgaptmp_004509"
     CDS             complement(4661..5584)
                     /gene="yicL"
                     /locus_tag="ACHOSM_RS22545"
                     /old_locus_tag="pgaptmp_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HGU4560862.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(5695..6879)
                     /gene="setC"
                     /locus_tag="ACHOSM_RS22550"
                     /old_locus_tag="pgaptmp_004510"
     CDS             complement(5695..6879)
                     /gene="setC"
                     /locus_tag="ACHOSM_RS22550"
                     /old_locus_tag="pgaptmp_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HGU4560863.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSIHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1785 a         1593 c         1788 g         1932 t
ORIGIN      
        1 attatcactg aaccgttgtt gtcatttgtt ttacaaaagc aagggattaa atctcctcca
       61 atggacaaaa aaatgaataa tgacaatacc gattacgtga gtaatgaatc agggacgctt
      121 tcgcgattat ttaaactacc tcagcatggg accaccgtcc gcacagaatt gattgcgggg
      181 atgaccactt ttttaaccat ggtgtacatc gtttttgtga acccgcaaat cctcggcgcg
      241 gcacaaatgg acccgaaagt ggtgtttgtt accacctgtt tgattgccgg tatcggcagt
      301 attgcgatgg ggatatttgc taacttaccc gtggcgctgg ctccggcaat ggggctgaac
      361 gccttctttg ccttcgtggt cgtgggggcg atgggcatct cctggcagac cgggatgggc
      421 gcaatattct ggggcgcagt tggactattt ttgctcacgc tgtttcgtat ccggtactgg
      481 atgatctcca acattccctt aagtttacgt attggtatca ccagcggaat tggattattt
      541 atcgccttaa tgggattaaa aaatactggc gttattgtcg ccaataaaga cacgctggtg
      601 atgattggcg atttaagttc tcacggcgtg ttgttaggta ttttagggtt ttttattata
      661 accgtgttgt catcacgtca ttttcatgcc gcggtgctgg tttctattgt ggtgacgtct
      721 tgctgtggat tatttttcgg tgatgttcat tttagcggcg tcattggtga agtagatttg
      781 agcggcgcgt taacacttga actcgccggt atcattttct cctttatgct gatcaaccta
      841 tttgattcat caggaacatt aattggtgta actgataaag cgggcttaat agatggtaac
      901 ggtaaattcc ccaatatgaa taaggcgctg tatgttgata gcgtcagttc ggtggcgggt
      961 gcgtttatcg gcacctcgtc tgttactgcc tatattgaaa gtacttctgg tgtggcagtc
     1021 ggtggccgca cggggctgac tgcggttgtg gttggcgtta tgttcctgtt ggttatgttc
     1081 ttctcaccgc tggtggcgat ggttcctcct tacgcaaccg ccggagcgtt aatctttgtt
     1141 ggcgtgctga tgacttcgag cctggcgcgc gttaactggg atgattttac cgaatcggtg
     1201 cctgcgttta ttaccacggt gatgatgccc tttactttct cgatcaccga agggattgca
     1261 ctcggcttta tgtcgtactg catcatgaaa gtgtgcaccg ggcgctggcg cgatctgaac
     1321 ctgtgtgtgg tggtggtcgc agctctgttt gcactgaaga ttattctggt ggattagtaa
     1381 ctggcgaggg tttccagata tcatgagttc tgattacgca ggagaactca tgatctggat
     1441 aatgctcgcc acgctggcgg tagtgtttgt ggttggtttt cgggtgctga catccggggc
     1501 cagaaaagcg attcgccgtc tcagtgatcg gctgaatatc gatgtcgtac ccgtggagtc
     1561 gatggtcgat caaatgggaa agtcagccgg tgacgaattt ttacgttatt tgcatcgccc
     1621 ggatgagtcg cacctgcaaa acgccgcgca ggtgttgctc atctggcaaa ttgtcattgt
     1681 cgatggtagc gaacagaacc tgctgcaatg gcatcggatt ttacaaaaag cccgtcttgc
     1741 cgcgccgatt accgatgctc aggtcaggct ggcgctgggt tttctgcgcg aaaccgaacc
     1801 tgaaatgcag gatattaatg cttttcagat gcgctataac gcgttctttc agcctgccga
     1861 gggcgttcac tggctgcatt aaaaccgctt cgtcttatca gcctacagac cgcgcctgac
     1921 ctgtcgggtg gataagacgt tcacgccgca tccggtaatc tttgccctgt tccgtttcga
     1981 tcttaactcc agctcaagca acaccgtaaa agttgcaaaa acgttaaatg catcacacat
     2041 ttcaatgtta aagtggtcgg cttttcccct gaaacatgcc acgggtaaca ccatgagtga
     2101 atttattgcc gaaaaccgcg gcgcggatgc catcacccga ccgaactggt cagccgtttt
     2161 ctcggtggcg ttttgtgtcg cctgtctgat tatcgttgag tttttgcccg tcagtttgtt
     2221 gacgccaatg gcccaggatt taggcatttc ggaaggggtt gccgggcaat cggtgaccgt
     2281 gaccgccttt gtggcaatgt ttgccagttt gtttattacc cagacaattc aggctactga
     2341 ccgccgctac gttgttattt tgtttgccgt tttgctgacg ctctcctgct tgctggtttc
     2401 ctttgctaac tcattcagtt tgcttttaat cggtcgtgcc tgtctggggc tggcgctggg
     2461 cgggttctgg gcgatgtcgg cgtcgctgac catgcgtctg gtgccgccgc gtacggtgcc
     2521 gaaggcgctg tcggtgatct tcggcgcggt ttctattgcg ctggtgattg ccgcgccgtt
     2581 gggcagtttt ttaggcgagc ttatcggttg gcgcaatgtc tttaatgcgg cggcggtgat
     2641 gggcgtgctg tgtattttct ggattatcaa atcattgcct tcactgccag gcgaaccctc
     2701 gcatcagaaa caaaatactt tccgcttatt acaacgtccg ggtgtgatgg cagggatgat
     2761 cgccatcttc atgtctttcg ccgggcagtt tgctttcttc acgtatattc gcccggtgta
     2821 tatgaacctg gcgggattcg gcgtggatgg cttaacgctg gtgctgttga gttttggtat
     2881 cgccagcttt attggtacgt cgctttcgtc gttcattctt aaacgttcgg taaaactggc
     2941 cttagcaggc gcgccgttaa tactggctgt gagtgcgttg gtactgacgt tgtggggaag
     3001 cgataaaatc gttgctaccg gcgtggcgat tatctggggg ctaacttttg cattggttcc
     3061 cgtcggctgg tcaacgtgga tcacccgctc gctggccgat caggcagaaa aagccgggtc
     3121 tattcaggtg gcggttattc agcttgctaa tacctgtggc gcggcaatcg gcggttatgc
     3181 gctggataat attggtctga cttcgccgtt gatgttgtcc ggcacattga tgttgctgac
     3241 tgcattgttg gttactgcaa aggtgaaaat gaagaaatcc tgatatcagg taaagaagcg
     3301 ttggtcagta gttgttgctg ttcttacata tccgggcatt ctaccttacc caacgcttcc
     3361 caataattgc tctgattatt acgttccatc gccacgatgg cctcgcgtat atgttgttta
     3421 ttgttttcat ctgacaacat taacttttcc cacgcttcat ctgataatga cgcctgtggc
     3481 gtgcaaattt tacgtaaatc ttgtaatacc gtggttttct cttttgcgga ctgcagtgag
     3541 ctaaaaggcg actcggcgta tacgatgcct ggcatagcga aaaaagtgaa aataagcagt
     3601 agtgtcgttg gtttcatgga ttcaaccttt tacaaatgtg tatcgaatga agcataagtg
     3661 tagtttgctt tcatcgctct catcatgctg tacgaaacgc gcttgcgtgg tcagtaagaa
     3721 gtgccagact ttatattcca cttttattcc tttttattct tatcgatagc gtttcgtttt
     3781 ttaaaccgca gcgaccttac cgctatagtc aggtaatcat taataaaagg ataaaaaaat
     3841 gaaactgaca acacatcatc tacggacagg ggccgcatta ttgctggccg gaattctgct
     3901 ggcaggttgc gaccagagta gcagcgatgc aaaacacatt aaagttggcg ttataaatgg
     3961 cgcagaacaa gatgtcgcgg aagtcgctaa aaaggtggca aaagagaaat atggcctcga
     4021 tgttgaactg gtaggattta gcggttcgtt gctaccgaac gatgcgacaa atcatggcga
     4081 acttgatgcg aatgtcttcc agcatcgacc ctttcttgaa caagataatc aggcgcatgg
     4141 ttataaactg gtagcggtgg gaaatacgtt tgtcttccct atggcgggtt attccaaaaa
     4201 aatcacaacc gtggcgcaaa taaaagaagg cgcgacagtg gcgatcccca acgacccgac
     4261 caaccttggt cgcgcacttt tactgttgca aaaagagaag ctaattactc tgaaagaggg
     4321 aaaagggtta ttacctaccg cgctggatat aaccgataat cctcgtcatt tgcagattat
     4381 ggaactcgag ggggcgcagc ttccgcgcgt actggatgat cccaaagttg atgtagcgat
     4441 tatcagcacc acttacattc agcagacagg gctttctccg gtgcacgaca gcgtatttat
     4501 tgaagataag aattcgccgt atgtgaatat tttggtggca cgggaagata ataagaatgc
     4561 agaaaacgtg aaggaatttc tgcaatctta tcaatcatcc gaagtcgcta aagcggcaga
     4621 aaccatcttt aacggtggcg cggtgcctgg ctggtaattc tcactcatcc cgcgccggac
     4681 gatttatttt tctggcgcgg cgacgggaat ccattgaaat caaaattacc gatgacagaa
     4741 tcagcagcgt tcccagccag tccggtaatg tgaacgtgat ccccagcaac agcaaagaga
     4801 gtagcgcgct acttaatggt tctgcacagc tcaaaatgct ggcttttggg ccgccaatta
     4861 attgtgctcc tttcaggtac aggctaaatg tcagagatgt accaatgacc accaaataaa
     4921 aaaacgccag aatcaaactg ccgttaacga caaagtttgt tccttgtctg gcataaaaag
     4981 gcaacagaat cagaccgccg atcagcatac tccagccgac gacaggcaac gtgccatagc
     5041 gggcaatcag cgttgaggga taggtggtat agaatgcagc agcaaatgcc gaagcaatgc
     5101 cccagaataa tgcggcagga gagatcgata atgacgttgg attaccgtgt gtcaccaata
     5161 aaaaagtccc gaccagcgat gtcaaaatag cgcaaaaaac cagaatgccc gggcgcgatt
     5221 tacgcaccag tgagaaccag gcgacgataa tcgttggtga gaggaattgc agcaccgttg
     5281 ccgtggctgc gttcgatttt tcgatggtta gcaaaaaagt gagctgtaca gttagcgcgc
     5341 caaccacgga aaaaatcagc aggctaatgg catctttatg attgttaata atagaaaaga
     5401 ttttatcgcc atgaacaaat gacaacgtca gcagaatcaa accggcgaat atcaaacgcg
     5461 tcatagtcaa aaactgcgac gacatctggc tttgctccat gatgtattgc gcgcagaccc
     5521 ctgaacttcc ccacaacacg gcggcaatca gaacgttcag catccccttt ctggtggaac
     5581 ccatttttcc ctctgcatac tgttttgtcg tttatgtatg caggcatgat agcaaaaatg
     5641 gcgaggatgg ctctgtttgc gccacgccga tttttcttag cccagattgg tgagctaaat
     5701 atctttaata aacagcaggc aaatcatcgc aatacccaac atccctatcg ccagccagaa
     5761 caacgcgtgg tagctccaga tttcaaccat aattccgtca acagagccgg cgataatcca
     5821 gccgacgcgt gaagtatttg catataacgt tgtcgcagag cctatttttt caggcatcag
     5881 gtcctgaaaa taaagcatgc cgataccaca gagaatacca aggaagatgg catttagaat
     5941 ttgcaattcc agctcaactg ccggagtcgt cgccatgagt acgctggcgt aaaaacacat
     6001 tccactcacg atagcaatga gcattaatag ccgcttgcca atacgtttca tgtaatagcc
     6061 tgcgattaac atcatcggaa tttccagacc ggcagcgata ccaatcattt ctccagccag
     6121 tttatcggtt agatgcagtt catcaataat aaatagcggc atatttatca tgtagagatt
     6181 attcgccgcc cacatcattg aacagaccac aaaaagtagc cgcgtatccc gctttctatg
     6241 gatggagggt aaaatttcta cgggttgggt aacgacagga atatttcttt gtattgatgg
     6301 caaaaacaac cagactatca gcccgcaaac aacaaatgcg atggcagcgg tgagatacat
     6361 cactttaaaa ctaaatccca ttgccagttc ataagcgagc ggtggcccga taacccaggc
     6421 aagcgagatc tgagcacgta aaaatgtact gaacataacc gtctcacggc ctgttctgtc
     6481 ggcgtgttca cgggcgaggg cgaacatttg cgggtttgcg gtagaagcaa aactactcag
     6541 aagtacgccc gttgagagca gaatgaagta gttgcgattc cacgcaaaaa gcgtacaggc
     6601 cagcactcca aataagcaac atagcaggat cagtaattta cggtcgcctt gtttatcgga
     6661 gtgccttgcc agaaattggc tgaccagaat tcccataata gcgctaccgg tgaagaaaaa
     6721 acctaccatt ataggacggg ctttcagttc atctgcgagg aatatactta gagtgggagt
     6781 ctggagagcg cccgcaatac cagtcagaaa ggcgacaagt aaaaatgccg cggcagtgag
     6841 atcaagtatt tttgatggag tggtggccgt tttttgcatt cttctgaatc accgggcgac
     6901 aaaatataac tgagttacaa ataagtacca gtcgaatgag tattgtatta agaatttttg
     6961 agtatcgctg atacaaccag aaagcaatgc tagcgcaata ttatagggcc agcaattgag
     7021 attattagca aaagcatgtt aagaattaat caccgaattt atatattaat tatagtaagg
     7081 caatgagcta tttttgat
//
LOCUS       DAZKHC020000172         7097 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZKHC020000172 DAZKHC020000000
VERSION     DAZKHC020000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.172"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            97..858
                     /gene="adiY"
                     /locus_tag="ACHOSM_RS22555"
                     /old_locus_tag="pgaptmp_004511"
     CDS             97..858
                     /gene="adiY"
                     /locus_tag="ACHOSM_RS22555"
                     /old_locus_tag="pgaptmp_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HGU4560864.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIQVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            995..2332
                     /gene="adiC"
                     /locus_tag="ACHOSM_RS22560"
                     /old_locus_tag="pgaptmp_004512"
     CDS             995..2332
                     /gene="adiC"
                     /locus_tag="ACHOSM_RS22560"
                     /old_locus_tag="pgaptmp_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HGU4560865.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2436..4079
                     /gene="eptA"
                     /locus_tag="ACHOSM_RS22565"
                     /old_locus_tag="pgaptmp_004513"
     CDS             2436..4079
                     /gene="eptA"
                     /locus_tag="ACHOSM_RS22565"
                     /old_locus_tag="pgaptmp_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HGU4560866.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4076..4744
                     /gene="basR"
                     /locus_tag="ACHOSM_RS22570"
                     /old_locus_tag="pgaptmp_004514"
     CDS             4076..4744
                     /gene="basR"
                     /locus_tag="ACHOSM_RS22570"
                     /old_locus_tag="pgaptmp_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HGU4560867.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4745..5845
                     /gene="pmrB"
                     /locus_tag="ACHOSM_RS22575"
                     /old_locus_tag="pgaptmp_004515"
     CDS             4745..5845
                     /gene="pmrB"
                     /locus_tag="ACHOSM_RS22575"
                     /old_locus_tag="pgaptmp_004515"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HGU4560868.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HYGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5821..5910)
                     /gene="pmrR"
                     /locus_tag="ACHOSM_RS22580"
                     /old_locus_tag="pgaptmp_004516"
     CDS             complement(5821..5910)
                     /gene="pmrR"
                     /locus_tag="ACHOSM_RS22580"
                     /old_locus_tag="pgaptmp_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HGU4560869.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6022..7097)
                     /gene="proP"
                     /locus_tag="ACHOSM_RS22585"
                     /old_locus_tag="pgaptmp_004517"
                     /pseudo
     CDS             complement(6022..7097)
                     /gene="proP"
                     /locus_tag="ACHOSM_RS22585"
                     /old_locus_tag="pgaptmp_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
BASE COUNT         1782 a         1809 c         1744 g         1762 t
ORIGIN      
        1 gcatgtttat attcatcata caattatata accatttccc ggtatcgctt tgctttagcg
       61 agaactggtc ttttattcgc actcaggagt acatgtatga ggatttgcag cgaccaacct
      121 tgtattgttt tattgactga aaaagatgtc tggataaggg tgaatgggaa agaacctatt
      181 agccttaaag ctaaccatat ggcgttatta aattgtgaaa ataatattat cgacgtctcc
      241 tctcttaaca acactttggt tgctcatatt agtcacgaca tcatcaaaga ttacctccgg
      301 tttctgaata aagatctctc gcaaatacaa gtatggcaac gtagcgctac gcccatactc
      361 accctgccat gcctgacgcc agacgtcttt cgcgttgccg cgcaacacag catgatgccc
      421 gcagaaactg agtcagaaaa ggaacgaaca cgcgcattat tattcactgt gctatcccgt
      481 tttctcgaca gtaaaaaatt cctttcatta atgatgtata tgttacgtaa ttgtgtaagt
      541 gacagcgttt atcaaattat tgaaagcgat attcacaaag actggaatct tagtatggta
      601 gccagttgtt tatgtcttag cccaagtctg ttaaagaaaa agttgaaaag cgaaaacacc
      661 agttatagcc aaataatcac cacctgccgc atgcgttatg ccgtaaatga attaatgatg
      721 gacggtaaaa atatctctca ggtatcacag tcctgcggct acaacagtac gtcgtacttt
      781 atttctgtct ttaaagactt ctacggtatg acgccgctgc attatgttag tcagcacaga
      841 gaacgcactg tcgcctgatt tttaacctta acgaagagct atattaataa cggcatcagc
      901 gataacccgg tcgataataa ttcaactatc gaatgcaggc gtatgatatg acgtaattta
      961 ttgtcacgaa gctcgccttc gcaggagttt aattatgtct tcggatgctg atgctcacaa
     1021 agtgggctta atccccgtca ccctgatggt gtcggggaat attatggggt caggtgtttt
     1081 tctgttacct gcaaacctgg cctccactgg cgggattgcc atttatggat ggttggtgac
     1141 gattatcggt gcgctggggc tctcgatggt atacgccaaa atgtcgttcc tcgacccaag
     1201 tcctggtggt tcttacgctt acgcccgccg ctgctttggc ccgtttctcg gttatcaaac
     1261 caacgtcctc tactggctgg cctgctggat cggcaatatc gccatggtgg tcattggcgt
     1321 aggatattta agttacttct tcccgattct gaaagatcca ttggtattaa ccatcacctg
     1381 cgtcgtggtg ctgtggatct tcgtcctgct gaacattgtc ggtccgaaaa tgatcacccg
     1441 tgtgcaggca gttgccaccg tgctggcgct gatccccatc gtcgggattg ccgtatttgg
     1501 ctggttctgg ttccgtggtg aaacctatat ggcggcatgg aacgtcagcg gcctgggcac
     1561 cttcggtgca attcaaagta cccttaacgt tacgctgtgg tcgttcatcg gtgtggaaag
     1621 tgcctccgtt gccgcaggtg tggtgaaaaa cccgaaacgc aatgtcccta tcgccaccat
     1681 tggtggggta ttgattgccg ccgtttgcta tgtactttct accaccgcga ttatggggat
     1741 gatccctaat gccgcactgc gcgtttctgc ttcgccattc ggtgatgccg cacggatggc
     1801 gttgggtgac accgccgggg ccattgtttc cttctgcgca gctgcgggtt gcttaggttc
     1861 actgggcggc tggacattgc tggcgggtca aacggcgaaa gccgctgcag atgacggact
     1921 gttcccaccg atttttgccc gtgtaaataa agcgggcacg ccagtggcgg ggctgattat
     1981 cgtcggtatt ttgatgacca tcttccagct cagcagcatt tcaccaaacg cgaccaaaga
     2041 attcggtctg gtttcttccg tctcggtcat ctttacactg gtgccatatc tttacacctg
     2101 tgcggcgtta ctgctgctcg gacacggtca ctttggtaaa gcacgcccgg catatctggc
     2161 agttactacc attgccttcc tctactgcat ctgggccgtg gtggggtccg gggcgaaaga
     2221 ggttatgtgg tcatttgtca ccctgatggt catcaccgcc atgtatgcgc tgaattacaa
     2281 ccggctgcat aaaaacccgt atcccttaga tgcaccaata agcaaagatt aattcccctt
     2341 aatccagcaa acataaaagc caaccttaag aacttaaggt tggcttaatt ttgctttgcg
     2401 agcatatgcg cactttgttc gatggaaaca ccgtgatgtt gaagcgccta ctaaaaagac
     2461 cctctttgaa tttactcgcc tggctattgt tggccgcttt ttatatctct atctgcctga
     2521 atattgcctt ttttaaacag gtgttgcagg cgctgccgct ggattcgctg cataacgtac
     2581 tggttttctt gtcgatgccg gtcgtcgctt tcagcgtgat taatattgtc ctgacactaa
     2641 gctctttctt atggcttaat cgaccactgg cctgcctgtt tattctggtt ggcgcggctg
     2701 cacaatattt cataatgact tacggcatcg tcatcgaccg ctcgatgatt gccaatatta
     2761 ttgataccac tccggcagaa agttatgcgc tgatgacacc gcaaatgtta ttaacgctgg
     2821 gattcagcgg cgtgcttgct gcgctgattg cctgctggat aaaaatcaaa cctgccacct
     2881 cgcgtctgcg cagtgttctt ttccgtggag ccaatattct ggtttctgta ctactgattt
     2941 tgctggtcgc cgcactgttt tataaagact acgcctcgtt gttccgcaat aacaaagagc
     3001 tggtgaaatc cttaagcccc tctaacagca ttgttgccag ctggtcatgg tactcccatc
     3061 agcgactggc aaatctaccg ctggtgcgaa ttggtgaaga cgcccaccgc aacccgttaa
     3121 tgcagaacga aaaacgtaaa aatttgacca tcctgattgt cggcgaaacc tcgcgggcgg
     3181 agaacttctc cctcaacggc tacccgcgtg aaactaaccc gcggctggcg aaagataacg
     3241 tggtctattt ccctaatacc gcatcttgcg gcacggcaac ggcagtttca gtaccgtgca
     3301 tgttctcgga tatgccgcgt gagcattaca aagaagagct ggcacagcac caggaaggcg
     3361 tgctggatat cattcagcga gcgggcatca acgtgctgtg gaatgacaac gatggcggct
     3421 gtaaaggtgc ctgcgaccgc gtgcctcacc agaacgtcac cgcgctgaat ctgcctggtc
     3481 agtgcatcaa cggcgaatgc tatgacgaag tgctgttcca cgggctggaa gagtacatca
     3541 ataacctgca gggtgatggc gtgattgtct tacacaccat cggcagccac ggtccgacct
     3601 attacaaccg ctatccgccg cagttcagga aatttacccc aacctgcgac accaatgaga
     3661 tccagacctg taccaaagag caactggtga acacttacga caacacgctg gtttacgtcg
     3721 actatattgt tgataaagcg attaatctgc tgaaagaaca tcaggataaa tttaccacca
     3781 gcctggttta tctttctgac cacggtgaat cgttaggtga aaatggcatc tatctgcacg
     3841 gtctgcctta tgccatcgcc ccggatagcc aaaaacaggt gccgatgctg ctgtggctgt
     3901 cggaggatta tcaaaaacgg tatcaggttg accagaactg cctgcaaaaa caggcgcaaa
     3961 cgcaacacta ttcacaagac aatttattct ccacgctatt gggattaact ggcgttgaga
     4021 cgaagtatta ccaggctgcg gatgatattc tgcaaacttg caggagagtg agtgaatgaa
     4081 aattctgatt gttgaagacg atacgctgtt attgcaggga ttgattctgg cggcgcaaac
     4141 cgaaggctac gcgtgcgatg gcgtgacaac cgcgcggatg gcggaacaaa gccttgaggc
     4201 cggtcattac agcctggtgg tactggattt agggttaccc gacgaagatg gactgcattt
     4261 tctcgcccgt atccggcaga aaaaatacac cctgccagta ctgatcctca ccgctcgcga
     4321 tacgctgacc gacaaaatcg ccgggctgga tgtcggtgcc gatgactatc tggtgaagcc
     4381 ttttgcgctg gaagagttac acgcccgtat ccgcgccctg ctacgacgcc ataataatca
     4441 gggcgaaagt gagctgattg ttggcaatct gacgctgaat atgggtcgcc gtcaggtatg
     4501 gatgggcggt gaagagttga ttctgacgcc caaagaatat gctctgctgt cacggttaat
     4561 gctcaaagca ggcagtccgg tgcatcggga aattctctac aacgacatct ataactggga
     4621 caatgaaccc tcgaccaaca ccctggaagt gcatatccac aatctgcgcg acaaagtggg
     4681 caaagcccgt atccgcaccg tgcgcggctt tggctatatg ctggtcgcga atgaggaaaa
     4741 ctaattgaat ctgatgcatt ttctgcgccg accaatatcg ctgcgccaac ggctgatatt
     4801 gaccatcggg gccattttgt tggtgtttga gctgatcagc gtcttctggc tatggcatga
     4861 aagtaccgag cagattcagc tgtttgagca ggcgctacgc gacaatcgca acaacgatcg
     4921 ccacatcatg cgtgagatcc gcgaggcggt cgccagcctg attgtccccg gcgtgtttat
     4981 ggtcagcctg acgctattta tctgctatca ggcggtacgc cgcatcaccc gcccgctggc
     5041 ggagctgcaa aaagagctgg aagcgcgcac tgccgacaac ttaacgccca ttgccattca
     5101 cagcgccacc ctcgaaatcg aagcggtggt ttcggcgtta aacgatctgg tcagtcgcct
     5161 gaccagcacg ctggataacg aaaggttgtt taccgctgac gtcgcgcacg aactgcgaac
     5221 gccactggcg ggggtgcgtt tgcatctgga actgctggcg aaaacgcatc acattgatgt
     5281 agcaccgtta gtggcacggc ttgatcagat gatggagagc gtctcccagc tgctgcaact
     5341 ggcgcgtgcc ggacagtcat tttcttccgg taattatcaa catgtaaaac tgctggaaga
     5401 tgtcattctc ccctcgtatg acgaactcag taccatgctc gaccagcgac agcaaaccct
     5461 gctattgcca gagagcgccg ccgacatcac cgttcagggt gatgcaaccc tgctgcggat
     5521 gttattgcga aacctggtag aaaacgccca tcgttacagc ccacaaggca gcaacattat
     5581 gattaagctg caagaagatg gcggagcggt catggccgtt gaagatgaag gaccgggtat
     5641 tgatgagagt aaatgcgggg agttgagtaa agcatttgta cgtatggaca gccgttatgg
     5701 cgggattggt ctggggttaa gtattgtcag ccgcattaca cagttgcatt acgggcagtt
     5761 tttcctgcaa aaccggcaag agacttccgg cacgcgggcc tgggtacggc tgaagaaaga
     5821 tcagaacgtg gcaaaccaga tataaagaaa gctgctgacc accagcacgc tgaacacggt
     5881 agttaaactt tcataaacac ggtttttcat tgcactctcc tccgttaacc tggaggagag
     5941 tatgcgcgtc agagattaag tgaaccttaa gagttcaatt ggcctgataa gacagcgtca
     6001 catcaggcca tccgtttcag cttattcatc aattcgcgga tgttgctgca ccaggcgggt
     6061 acgtttcgcc tgcaaatcgg caatctcgtg gtcaatatca tcgattttct gctcgatatt
     6121 atcgtaatgc tcgacgagaa tttccttcgc ttcctgtata tctgacgccg ccggtgtcgc
     6181 acctttcaac ggacgatttg ccgtctcttt catggttacg ccggtgatta aaccaatcac
     6241 cgccactacc atcaggtaat aggcaggcat catcagattc tggtagaagc cataacgccc
     6301 gtaaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag accgatgacg
     6361 ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagggcaac actaccaagt
     6421 agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg ctggacaaac
     6481 agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga gtaatgcagg
     6541 ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac gttggtggca
     6601 ataaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat ctctttaaac
     6661 gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt atcaacatgc
     6721 tgctgaaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat aatccctaac
     6781 ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc ttcgccgaca
     6841 atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat agaaccgaag
     6901 tccagccagc tgcccataaa gccacgttta cggtcagggg agtattccgc aacaaatatc
     6961 gacgccccgg tatattcacc gccgaccgag aaaccttgtg ccatcttaca gatcaacagc
     7021 agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat acagaacgta
     7081 ctgatcgaca taatcac
//
LOCUS       DAZKHC020000173         6970 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZKHC020000173 DAZKHC020000000
VERSION     DAZKHC020000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.173"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            81..1139
                     /locus_tag="ACHOSM_RS22590"
                     /old_locus_tag="pgaptmp_004518"
     CDS             81..1139
                     /locus_tag="ACHOSM_RS22590"
                     /old_locus_tag="pgaptmp_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGU4560870.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            1149..2291
                     /locus_tag="ACHOSM_RS22595"
                     /old_locus_tag="pgaptmp_004519"
     CDS             1149..2291
                     /locus_tag="ACHOSM_RS22595"
                     /old_locus_tag="pgaptmp_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HGU4560871.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2284..3057
                     /locus_tag="ACHOSM_RS22600"
                     /old_locus_tag="pgaptmp_004520"
     CDS             2284..3057
                     /locus_tag="ACHOSM_RS22600"
                     /old_locus_tag="pgaptmp_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560872.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3059..4138
                     /locus_tag="ACHOSM_RS22605"
                     /old_locus_tag="pgaptmp_004521"
     CDS             3059..4138
                     /locus_tag="ACHOSM_RS22605"
                     /old_locus_tag="pgaptmp_004521"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HGU4560873.1"
                     /translation="MQPHETFTGSYQPSDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4138..5094
                     /locus_tag="ACHOSM_RS22610"
                     /old_locus_tag="pgaptmp_004522"
     CDS             4138..5094
                     /locus_tag="ACHOSM_RS22610"
                     /old_locus_tag="pgaptmp_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HGU4560874.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            5105..6313
                     /locus_tag="ACHOSM_RS22615"
                     /old_locus_tag="pgaptmp_004523"
     CDS             5105..6313
                     /locus_tag="ACHOSM_RS22615"
                     /old_locus_tag="pgaptmp_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HGU4560875.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPVESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSRMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6331..6798
                     /gene="terW"
                     /locus_tag="ACHOSM_RS22620"
                     /old_locus_tag="pgaptmp_004524"
     CDS             6331..6798
                     /gene="terW"
                     /locus_tag="ACHOSM_RS22620"
                     /old_locus_tag="pgaptmp_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HGU4560876.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
BASE COUNT         1665 a         1797 c         1867 g         1641 t
ORIGIN      
        1 atgattgaca tgtagcttag acactcatag actcacttct caatcataaa tttgcatggc
       61 gttccggata attaaaaaaa atgaacaaga aaatttggtt tatggaaggt ttatcctccc
      121 agcgagatat tattcagggg gtaaaatcat ttgcacaaaa aaataatttt gccattaccg
      181 tttttgcctc ccaccgtaac gaaagaaatg aaatcctttc cgttgccgat tattctttga
      241 ctgaacctga agatcctcaa aaacgtcttc agtttatcca ggaaaccatt cagacctacg
      301 gcatccacca tattcatact ggccgtaaca gccagtggtt tgaagaacac cgttcagcca
      361 ttgaaccgac cggtgccacc cttactaccg gtgcaacggg cgtcgactgg ttaactctgg
      421 ctgacgaaaa agttactttt gctcagttta tggagcaaaa gggtctcccg gtcgtaccat
      481 cctggcgggt gaatacgctg gcagaattaa agacacacct cgcggccccg ccgttcactg
      541 acagcccggt atgcgtgaag ccggtgacgg gtatctatgg catgggattc tggcgctttg
      601 atgacagtgc ttcgcctatg gccgtcttta atcatcccga acatcgtctg gtcagtccgc
      661 aacagtatat tgcagcagca tcagctgctg agtcgtttaa accccttgtt ttgatgccgt
      721 acctgccagg cccggaattt tccgtcgata tcctcgcgga taagggcgaa atactcgcag
      781 ccgtgggacg ccgtaaggaa ggggctatcc agtatctggt aaacgaagga agcgcctggg
      841 aactggcgtg tgactgcgcc cgtgttatga aggccgacgg gctggtgaat gttcagacgc
      901 gaaacgatgt gaatggcaac ccggtgctgc ttgaaaccaa catgcgtccg tcaggggggg
      961 tgggttatac ccttcacagc ggtgtgaacc ttcctgggtt atttgctgcc tttaagctcg
     1021 gtctgatgtc tgaagatatg gtacgccaga gcgctaaaaa caccttttct ccggttgcgg
     1081 tgagatccat tacggatgta attgcatacc cggaatcact ctctaacctt ctgaattaag
     1141 gcgtatttat gtctgatatc ctcgaagata tgatttatcg ccgtaccctc tcatgcggta
     1201 cgattcaggt aacccgtgac cagggtgaag tttcgctcga tgacctgttt gacatcgctg
     1261 aaagacgtaa cccgaaacgc gcctttctgt ttgtcagcaa agtactcggc aggcacattc
     1321 ccgtgccacc ctcagtcatg cggcaggcct acagacagct tgccagccag ttcccctcga
     1381 cactgacagg acccgtactg tttatcggta tggctgaaac cgccgttggg cttggggccg
     1441 gtgtatttga tgaagtgcgc caccagcatc gtgaatctgt ctatctgacc tctacccgtc
     1501 acccggttga tggcacgttg ctttgcgagt tcaaggaaga acacagccac gccaccgatc
     1561 atctgatcta tctgccagat gatgaagaga aaagacgtcg tgttaccaac gcacgaacgc
     1621 tggttttgat tgatgacgag gcaaccaccg gtaatacctt tattaacctg ctttctgccc
     1681 tgcgtaatac cggcaagctt caacatattg aacaggttat cgccgtaacg ctgaccgact
     1741 ggagtggcaa tgccctgtcg gagcgcaccc ccttgcccgt cacttcggtt tcgcttgtaa
     1801 gcggtcagtg gggatggacc ccattacctg atgcacctgt cccggacatg ccgaaagtca
     1861 acgtaacttc acgaggagaa tgggacatcc aggggaaaca gtcatggggc cgactgggga
     1921 tgctcgcacc tgcggccgat ctcggccatg aggtctccgt ccacaagggg gaacgtattc
     1981 tggttctcgg gaccggggaa ttcgtctggg agccgttcct gcttgctgaa cggctcgaag
     2041 ctgccggagc acaggcattt tatggatcga ccacccgctc ccctgtcgcc gttggttatg
     2101 ccattgagtc cgccatttcc tttacggata actacgggct gggcatcccc aattttgtct
     2161 ataacgtcgc ccaccagcag tttgaccgca ttcttgtgtg tactgagaca cccgcagaaa
     2221 gtattgacac gcagcttctt aaagcgctgg ctgaggttgc gcccgtcgtg gagattgtta
     2281 cctatgaata aacccgttgt tctgagcgac cttgacgaca cgctctttca gacccgtcgt
     2341 aaaatggtgg acgagctggc cctggagcca ttccgtaccg gtgccgttga tcgcaccctg
     2401 aatccacgaa gctttatgac ggaagaacag tccatgttgg tggactggct cctggagcag
     2461 gctgaactca tacccgttac cgcacgggga actgaagaaa tcagccgcgt acggatccct
     2521 ttccactcct gggcaatcac aacgcacggg gccgtcattc tcacgcctga aggaaaaccc
     2581 gacgaggagt ggaaggccca tatgctcggc cagctggctc cctaccagga aaagctgaca
     2641 tcgatgcagc gtctgatcac tgaaatgatg gacgcaaaag ggatcaatgc ctgggcaagg
     2701 ctgaacttcg aatacggtga aacggcggtt tatatggtga tgaaacaccg cgacagcacc
     2761 cgccttgacg agctcaatgc cattgcagat gagatagaaa cggtgtttcc gaccgagggc
     2821 ttctacatcc accgcaacag taataacgtg gcctggcttc ccaccccggt tgagaaaggg
     2881 ctggcagtca gctggctgct tgaaaaactt cgggctgaac gaggagtttt ccccgtaatt
     2941 ggtttgggcg acagcctgag cgatcatcgt tttatgaaac tgtgcagctg gtttggcatc
     3001 ccgcgtcaga gtcagtttgc agatgccatt tcacagcgaa tttttggaga aaattaaaat
     3061 gcagccacat gaaacattta ccggctcata ccagcccagt gacgtggaat ttctgctaaa
     3121 gccggttgtc attgagatga cgccggttga gaaaaaagaa gaactgattc agtccgggaa
     3181 gaaacattat tcggacatgc tcagccagga gccagcgcct acacaatggc atcttgattt
     3241 gtttcaccgg gcgctggatc ggggcgcaga gaggcttgca aaagaagtca cacagctcgc
     3301 tattgctctg gccgaacgct tcggtgatga gcccattgta ctggccagtc ttgtcagggc
     3361 tggcgttccg ctcggcgtta tgctgcacca ggccctgcgt gacatgggga aaacctcatg
     3421 gcattacggt atcagcatta tccgggatcg tggaattgac ggtgcagccc tcgacgtcat
     3481 tgaagagcgg catggtacca gcggtattgt ctttgttgac ggatggacag gtaaaggcgc
     3541 aattaccgga gagcttgtac gcgcactgaa agatcgcccg ggctatcctg agcagccgcg
     3601 actggttgtc ctggccgatc cctgtggctg ctcctggctt gcagccagtg atgatgactg
     3661 gctcattcct tttggcatca tgggggcgcc ggtatcaggc ctgatctccc ggtcagtatg
     3721 gtcctctgaa ggattacacg ggtgcatggt ttgcgagcat ctcagtgaat tcgaatgcag
     3781 ccggatgctt gccgataccg tcgctcattt ccgtaagaag ttaacaccgt catccctcgc
     3841 tcccctgagc tggaatacgg aatcagcccg ggtcttatgg cagacaagtc gcgacgttat
     3901 cgcgttcctg gccgatgaat ttaaagtgga cagcgtcaat cgtattaaac ccggtattgc
     3961 tgaagcaacc cgggctgtat tacgtcgggt accggaccat gtatttgtgc gttctattga
     4021 cgacccggac gttgccttgc ttgtagggct tgctcgtgaa aagggaatag ttgttacaga
     4081 aatgggggga accctcggcc agtatcgggc tgtaaccatt atcaagaagg tactctgatg
     4141 aaaaatcgtc tttctccctg gaatctggga gccacgctat acatgcctgc aacacgggaa
     4201 gatattgctg atgccgtcct gcacgggaaa atcccgggct tgcgctctct ggtgatttgc
     4261 ctggaggatg ctgtcagtga agcagacatc cccgtcgccc tcaaaaatct ggagcacctg
     4321 ctgcacgagc tcagtaacag catgagtagc ctgggtaaaa atgactggcc tctggtgttc
     4381 attcgccccc ggcatgccga aatgggcaga tggttaaaag cgcattatga tctttccgct
     4441 gtcgacgggt ttgtgctgcc taaatttacc ctcagttcgt tagctgaatg gtgggatatc
     4501 atggtcggga ctcacctgtg catgatgcca acgctggaaa cagaagatgt ctttgacgtg
     4561 gttcagatgc gcgagctggc tacccgtctg gaggaacatc cctgccacga ccgcattatt
     4621 gcgcttcgaa tcggcggcaa cgatcttatg aacgttgtgt cgcttcgccg tccccgggac
     4681 ctgacgctgt atgacagccc gatgggctac gtcatcaaaa tgctcgtttc ggtcttcggc
     4741 ccacgtgatt ttgccctgac cgcgcctgta tgtgagcata ttgacgatca tgccgttatg
     4801 gccagagagc tggctcttga tatggcacac gggcttgttg ggaaaacggc cattcacccg
     4861 ggtcagatag aggtcattca aaacgcgctg atggtcactc agggtgagca ttctgacgct
     4921 ctgcggatcc tgaactctac ccaggccgtg tttaagtcgc agggagcaat gtgtgaaccc
     4981 gccacacatc gccgctgggc ggctggcatt ctggacaggg ctcgttttta tgggttacag
     5041 aacgagcaaa gcgctgatgg aatcagatta cttaccgtga cccagcatca ttaaggaaat
     5101 cataatgggg ttcaggttca gaaagtcgat caacattatt cctggcgttc gcctcaacct
     5161 gagtaacggt gcaccgagcc tgagtgtcgg gccgagaggt gcttccgttt cttttggtag
     5221 ccgggggacc tatgccaatc tgggcttgcc cggtaccggg ctgagttacc gtacccggct
     5281 tgaccgggcc gcgcgttcca gaggtgaaaa ccggacggca accgacccgg ggctcagaca
     5341 ggcgcttgag cagaaagccg ctgaactcat gtcagcggta accgcaatcc gtaatattca
     5401 cgagctgacg ccggatccaa aaacaggcat cagctgggca gagctggaag cagtatacct
     5461 gcataacaga acgtcgcctt ttcaggttcc ggcaccggtg cgtccagaaa agccagacta
     5521 ccttgtattg ccggaaaagc ctgtcgagag cgaaggcatt agttttttgg gtaaatggtt
     5581 tgaatcggaa tcagctaaag ctgagcgcca cgccgaaaat cttcgccggt ggcagcagga
     5641 gctgattgat gtggagcgtg agaataccct tcgacagcac cggtaccagc aacaacggac
     5701 ggcctgggcc gaacagtatg caaactggaa gtttgaagct gaagaacatg aaaaacggct
     5761 cgccacggct caggcagatg cccggcagca gttccggaca gacgccgcgt ttttcgaatc
     5821 atacctggcg ggtgtgctgg cagaaactga atggccgcgt gaaacgcttg ttgcatttga
     5881 agtaaagccg gagctatcag cagtcctgct ggacgttgat ttagctgaga ttgaagattt
     5941 ccctgataag atttacggcg ttaatgcccg gggaacggag ctgacggaaa aagccatgac
     6001 gcaaaaagcc gtacgcgaaa actatgccca ccacgtccat ggctgcttgt tccgcctggt
     6061 cggtatcgtt ttacatacgc tacctttcga caacgtgatt gtgtcaggct ttacgcaacg
     6121 ggtcagtaag cggaccggct atctggagga tgagtatatc ctgtcctgca aatgcactcg
     6181 cagccggatg tcgtcagtaa attttgcagg cataaaacac attgatccgg ttgaagcgtt
     6241 aggcgatcaa ccggttattc gaaagatgag cagtaccttc atttttcagc ctattgaacc
     6301 actaaccctt taagcaggtt aaatcttacg atgcaattaa ataccagaca ggcccggatt
     6361 tttaagcttg ccaacttact gggcaccggc aagcccgttt ccgctgccga cattataacc
     6421 agtctggaat gctctgagcc gacattaacc cgggccctca aagagctacg cgagtcatat
     6481 tctgcggaga tcaaatacag caaggcaggt cattcctatc atctcgttaa tccgggccag
     6541 ttggataaaa agaccctccg ccggatgaat gaagcgctcg cacagaatgc tgaactgaaa
     6601 accggtgagt ccacagggaa ggtcgtactc gataaggata aaaaaacagc cgtgtcatta
     6661 tcgctacgca tgcggattct aagaaaaatt gaccgtctgg ctgcgctgag tgactcgacc
     6721 cgaagtgaag cggtagagaa gctggctctg cactccgttg atgaactgat aaaagaatac
     6781 agcgctaaaa agtcctgacg ctaaatctca gaccggggtg aaaactccgg ttttgtcata
     6841 tttcacctct gtattggcgt gatgcaaaat ggcatctgaa tcgatcatgg tttggcatcc
     6901 atttccgatc acagtttggc atcacaatcg atcacgattt ggcatgcttc cgctcattga
     6961 ttggcatcct
//
LOCUS       DAZKHC020000174         6845 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZKHC020000174 DAZKHC020000000
VERSION     DAZKHC020000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.174"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1808
                     /locus_tag="ACHOSM_RS22625"
                     /old_locus_tag="pgaptmp_004525"
     CDS             <1..1808
                     /locus_tag="ACHOSM_RS22625"
                     /old_locus_tag="pgaptmp_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4560877.1"
                     /translation="FRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVY
                     QTTVPPGPFTIDDINSATNGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALA
                     MGEYRSGNNLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAF
                     SFDITQANTTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYS
                     RMSGYTVKPPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYW
                     NTSRSDQQISFGLNLPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQ
                     SAFRNSNASYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYS
                     SLNYRGAYGNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGAD
                     NVKIENQTGIHTDWRGYAILPFATEYRENRIALNANSLADNVELDETVVTVIPTHGAI
                     ARATFNAQIGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGQL
                     QVSWGKDKNSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            1822..2352
                     /gene="ydeS"
                     /locus_tag="ACHOSM_RS22630"
                     /old_locus_tag="pgaptmp_004526"
     CDS             1822..2352
                     /gene="ydeS"
                     /locus_tag="ACHOSM_RS22630"
                     /old_locus_tag="pgaptmp_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4560878.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGGNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            2365..2868
                     /gene="ydeR"
                     /locus_tag="ACHOSM_RS22635"
                     /old_locus_tag="pgaptmp_004527"
     CDS             2365..2868
                     /gene="ydeR"
                     /locus_tag="ACHOSM_RS22635"
                     /old_locus_tag="pgaptmp_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4560879.1"
                     /translation="MKRLHKRFLLATFCSLFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            2927..3836
                     /gene="ydeQ"
                     /locus_tag="ACHOSM_RS22640"
                     /old_locus_tag="pgaptmp_004528"
                     /pseudo
     CDS             2927..3836
                     /gene="ydeQ"
                     /locus_tag="ACHOSM_RS22640"
                     /old_locus_tag="pgaptmp_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4171..6450
                     /gene="ydeP"
                     /locus_tag="ACHOSM_RS22645"
                     /old_locus_tag="pgaptmp_004529"
     CDS             4171..6450
                     /gene="ydeP"
                     /locus_tag="ACHOSM_RS22645"
                     /old_locus_tag="pgaptmp_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HGU4560880.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVTGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPFSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            6698..>6845
                     /gene="safA"
                     /locus_tag="ACHOSM_RS22650"
                     /old_locus_tag="pgaptmp_004530"
     CDS             6698..>6845
                     /gene="safA"
                     /locus_tag="ACHOSM_RS22650"
                     /old_locus_tag="pgaptmp_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HGU4560881.1"
                     /translation="MHATTVKNKITQRDNYKVIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMG"
BASE COUNT         1908 a         1511 c         1599 g         1827 t
ORIGIN      
        1 actttcgtgg cctcaaaata aattcaacag aagcgatgtt gcccgatagc caacatggtt
       61 tcgctcctgt gattcatggt attgcccgtg gaaccgcaca agtgagtgta aaacagaacg
      121 gatacgatgt ttatcagact actgttccac ccggcccttt tactattgat gatatcaact
      181 ctgcgaccaa tggtggtgat ctgcaagtaa ccataaaaga ggcagacggc agtattcaga
      241 cattatatgt tccttattcg tctgttccgg ttctccaacg tgctggatat acgcgttatg
      301 cgcttgccat gggggaatat cgtagtggaa ataacctgca aagctctccc aagttcatac
      361 aaggtagctt gatgcatggt ctggaaggaa actggacacc ttatggcgga atgcaaattg
      421 cagaagatta tcaggccttc aaccttggta ttgggaaaga tttaggactt tttggtgcct
      481 tttctttcga tatcacgcag gccaatacga cacttgcaga tggcacccgt cacagtgggc
      541 aatcggttaa atccgtctac agcaaatcct tctaccagac aggaaccaat atccaggtcg
      601 caggatatcg ctattctacg caaggttttt ataacttatc cgacagtgcc tacagtcgaa
      661 tgagtggtta caccgtcaag cctcctaccg gagacaccaa tgagcagaca caatttattg
      721 attattttaa tctgttctac agtaagcgtg gtcaggaaca aataagcatc tctcagcagc
      781 ttggaaatta cggtacgaca tttttcagtg ccagtcgcca aagttactgg aacacgtcac
      841 gcagcgacca gcaaatatca tttggattaa atttgccgtt tggtgatatt acgacttcgc
      901 tgaattacag ctattccaat aatatatggc aaaacgatcg ggatcattta ctcgctttta
      961 cgcttaatgt tcccttcagt cattggatgc gtacagacag tcagtcggca tttcgtaatt
     1021 caaacgccag ttacagtatg tcaaacgatt tgaaaggcgg catgaccaat ctatcggggg
     1081 tttatggcac tctgctgccg gataataacc tgaattatag cgttcaggtc ggtaacaccc
     1141 acggaggtaa tacatcgtct ggcaccagtg gttacagttc tcttaattat cgtggagctt
     1201 atggtaatac taatgtcggt tacagtcgga gtggtgacag cagccagatt tattacggaa
     1261 tgagtggtgg gattattgct catgctgatg gcatcacctt tggacagccg ctgggcgaca
     1321 caatggttct ggttaaggct cctggtgctg ataatgtcaa aatagagaac cagaccggaa
     1381 ttcataccga ctggcgtggc tatgccatat taccatttgc gacagaatat agagaaaacc
     1441 gtattgctct taacgcgaat tcccttgcag ataatgttga actggatgaa accgtggtca
     1501 ctgtcatccc aactcacggt gctattgcca gagcaacatt taatgcacaa atcggcggga
     1561 aagtattaat gacgttgaag tacggtaata agagcgttcc attcggtgca attgtcacac
     1621 acggagagaa taaaaatggc agcattgtcg cggaaaatgg tcaggtttat ctgactggac
     1681 ttccacagtc agggcaatta caggtttcat ggggcaaaga taaaaactca aactgtattg
     1741 tcgagtacaa gcttcctgaa gtttctcctg gtaccttact gaaccagcag acagcaatct
     1801 gtcgctaagg aacaatagat tatgaaatac aataacatta ttttcctcgg tttatgtctg
     1861 gggttaacca cctattctgc tttatccgca gatagcgtta ttaaaattag cgggcgcgtc
     1921 ctcgattatg gctgcacagt ctcatcggat tcgcttaatt ttaccgtaga tctccaaaaa
     1981 aacagtgcca gacaatttcc aacgaccggt agcacaagtc cagccgtccc ttttcagatt
     2041 acgttaagtg aatgcagcaa agggacaacg ggggttcggg ttgcatttaa cggtattgag
     2101 gatgcagaaa ataatactct gttgaaactg gatgagggag gcaatacggc ctccggtttg
     2161 ggtatagaaa tactggacgg aaatatgcgt ccggtgaaac tgaatgacct tcatgccggg
     2221 atgcagtgga tcccactggt accagaacag aacaatattt tgccttactc cgctcgtctg
     2281 aagtcaactc agaagtccgt caatccggga ctggtgaggg cttcggcaac ctttaccctt
     2341 gaatttcaat aatcagagtc agatatgaaa agacttcaca agaggttcct gttagctacg
     2401 ttttgctcgt tattcacagc aactctccag gccgccgatg tcactatcac tgttaatggt
     2461 cgggtagtcg ctaaaccctg cactattcaa accaaagaag ctaacgttaa tctcggggat
     2521 ctttatacgc gcaatctgca acaacctggt tctgcatctg gctggcacaa tattactttg
     2581 tcattaaccg attgtccggt tgaaacaagt gcagtgacgg caatcgtgac aggttcaact
     2641 gacaatacgg gttattacaa aaatgaaggt actgccgaaa atattcagat agagctgagg
     2701 gatgaccagg atgctgcgtt aaaaaatggc gatagcaaaa cggttattgt tgatgagatc
     2761 actcgtaatg cacagtttcc acttaaggca agagctatca cggtgaatgg aaacgcaagc
     2821 cagggaacga tcgaggcgct aatcaatgtg atctacacct ggcaataacg tcgattcgat
     2881 tttcttagcc ttctttttaa atcaattttt aaccttagga tatatcatgg gtaaaacaat
     2941 cagtataaag gtcctgttcg gtatttatct tctattaatg gcaggcaaag tttttgcctt
     3001 cagttgtaat gtcgatggcg ggtcaagtat tggagcaggt acaacctctg tttatgttaa
     3061 tctcgaccct gtaatacagc cgggccagaa tctggttgta gacttgtctc agcatataag
     3121 ttgctggaat gattacggcg gctggtacga cactgatcat ataaacctgg tacaaggctc
     3181 agctttcgca ggctccctac agtcatataa aggatcgctg tactggaata atgtgaccta
     3241 ccccttcccg ttgacgacaa ataccaacgt attggatatt ggtgataaga caccgatgcc
     3301 attaccgtta aaactctata ttacctgggg cggccggtgg agttgtcata aaagcggggg
     3361 aagtcattgc ccgtattcat atgtataaaa ttgcaacatt aggaagcgga aatccgcgta
     3421 attttacgtg gaatattatt tcaaataaca gtgttgttat gccaacaggc ggctgcaccg
     3481 ttgatagtcg taatgtcact gtggatctcc cggactttcc tggaagcgca gaaatacccc
     3541 ttggcgtata ttgctcaagc gaacaaaagc tctcatttta tctttcgggt gccaccactg
     3601 acagtagccg acaagtgttt gctaacacag cacctgatgc aacaaaagca tcgggagtgg
     3661 gtgtctctct gatgcgtaat ggaaaaatac tggctacggg agaaaatgtc tccctgggga
     3721 cagtaaataa aagcaaagta cctctgggtt taagcgcaac ctatggacaa acaggaaaca
     3781 aggttgcagc cggaaccgtc cagtcagtca ttggtgtcac ttttatctat gaataattgt
     3841 gattttgatc ttagtctatg cgctccattc acgaaaattg cgcggataat tattttgtga
     3901 aggctattat cctacacctg taagaaaatc cgcgcacaat tgaagccaga gtcttatttt
     3961 tttatatcga aaaatatgtt aatttaaaca ctatgagaga tgatgcttaa cttatcgtat
     4021 ttaatctatt gtttaacgat aatagattaa aaatagaaag agagtagtcc cctactctta
     4081 tatatccatg ttggcgataa tccctttgta tgtactgtgc atcatcgcta ttacaaatcc
     4141 taataattca tttccacaca ggataagtag atgaagaaaa aaattgaatc ctaccagggt
     4201 gctgcaggtg gttggggtgc tgttaaatcc gtagcgaatg cagtacgtaa gcagatggat
     4261 atacgccagg atgttattgc catgtttgac atgaataagc cagagggctt tgactgtccg
     4321 ggttgtgcat ggccagatcc taagcacagt gcgtcattcg acatttgtga aaacggcgca
     4381 aaagcaatcg cctgggaagt cacggataag caggtaaatg cctctttctt tgctgagaat
     4441 acggttcaat cattacttac ctggggagat cacgagcttg aggctgcggg acgtctcact
     4501 cagcctttga aatatgatgc cgtcagcgac tgttacaagc cattaagctg gcaacaagct
     4561 ttcgacgaaa ttggcgcacg ccttcaaagc tatagtgatc ccaatcaggt tgaattctat
     4621 acttcgggcc gcacttccaa tgaagctgcc tttctttatc agctttttgc ccgtgaatac
     4681 gggagcaata actttcccga ctgctccaac atgtgccatg aaccgaccag tgtgggtttg
     4741 gcagcgagta tcggtgtggg taaagggact gtgttgctgg aagactttga gaagtgcgat
     4801 ttagtcattt gcattgggca taaccctggt acaaaccacc ctcgcatgct gacttcgttg
     4861 cgcgctttag tgaaacgggg agcgaaaatg atcgccatca atcctctaca ggaacgtggc
     4921 ctggagcgat ttaccgcacc gcaaaacccg tttgaaatgc tgacgaactc tgagactcag
     4981 ttggccagtg cctactacaa cgtgcgcatt ggtggcgata tggcgttgct caaggggatg
     5041 atgcgcctgt taattgagcg cgatgatgct gcaagtgccg caggtcggcc ctcattgctt
     5101 gatgacgaat ttattcaaac gcataccgtc ggctttgacg agctacgccg tgacgttctc
     5161 aattccgagt ggaaagatat cgaacgtatt tctggactaa gtcagacaca aatcgccgaa
     5221 ctggctgacg catatgccgc tgccgatcgc accattatct gttacggaat ggggatcact
     5281 cagcacgaac atggtaccca gaatgtacag caactggtca atctgctgtt gatgaaaggt
     5341 aacattggca agcctggtgc gggtatctgc ccactacgag gtcactctaa tgtacagggc
     5401 gaccgaaccg tcggtatcac cgagaaaccg tctgcagagt ttctggctcg cctgggtgag
     5461 cgctatggct tcaccccacc tcatgcacct ggacatgctg caattgccag catgcaagca
     5521 atatgcacgg ggcaggctcg agcattgatc tgcatggggg gcaactttgc actggcaatg
     5581 ccagatcggg aagcgagcgc tgtaccgtta acgcaattag atttggcggt acacgtagcc
     5641 actaagctta accgctctca tctgctgacc gcacggcata gctatattct gccggttctg
     5701 gggcgtagcg agattgacat gcaaaaaagc ggtgcgcagg cggtaaccgt tgaggattca
     5761 atgtcgatga ttcatgcctc gcgtggcgtg ttaaaacccg ccggtgtaat gctgaaatca
     5821 gagtgtgcag tggtcacggg aatcgcgcag gcagcactac cccagagcgt ggtagcctgg
     5881 gagtatctgg tggaagatta tgatcgcatt cgcaatgaca ttgaagctgt gttaccagag
     5941 ttcgccgact ataaccagcg catccgacat cctggaggtt ttcacctgat aaatgcagct
     6001 gctgaaaggc gctggatgac gccgtcaggt aaggctaatt tcattaccag caaagggctg
     6061 ttagaagatc ccttttcagc gtttaacagt aagctggtca tggcgacagt acgcagccac
     6121 gatcagtaca acacgacgat ttatggtatg gatgatcgct atcgaggggt attcggtcaa
     6181 cgagatgtgg tctttatgag tgctaaacaa gctaaaattt gccgtgtaaa aaacggcgaa
     6241 agagttaatc ttattgcgct tacgccagac ggtaagcgca gctcacgccg catggataga
     6301 ttaaaagtgg tcatttaccc tatggctgac cgctcactgg tgacctattt tccagaatcg
     6361 aatcacatgc taactcttga taaccacgat ccattaagtg gcattcctgg ttataaaagt
     6421 attccggttg aattagaacc atcaaattaa ttcgcctcct catttcttct gctgtcatcc
     6481 gcacagcaga agaatttctc attgactatt atcgcgcaat ttgctcacat tgattaaatt
     6541 aaaccacaca ctataagata taaacttctg cctacagctg taagaaactc cgctcagtac
     6601 tgaagcacca gtcctatttc ctcttttctc cagcctgtta tattaagcat actgattaac
     6661 gatttttaac gttatccgct aaataaacat atttgaaatg catgcgacca cagtgaaaaa
     6721 caaaatcacg caaagagaca actataaagt aatcatgtct gtaattgtgg ttgtcttatt
     6781 actgacactt acgttgatag ccattttttc ggcaattgat cagctgagta tttcagaaat
     6841 gggtc
//
LOCUS       DAZKHC020000175         6775 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZKHC020000175 DAZKHC020000000
VERSION     DAZKHC020000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.175"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            89..331
                     /gene="yjbE"
                     /locus_tag="ACHOSM_RS22655"
                     /old_locus_tag="pgaptmp_004531"
     CDS             89..331
                     /gene="yjbE"
                     /locus_tag="ACHOSM_RS22655"
                     /old_locus_tag="pgaptmp_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HGU4560882.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            518..1156
                     /locus_tag="ACHOSM_RS22660"
                     /old_locus_tag="pgaptmp_004532"
     CDS             518..1156
                     /locus_tag="ACHOSM_RS22660"
                     /old_locus_tag="pgaptmp_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HGU4560883.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGASWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1153..1890
                     /locus_tag="ACHOSM_RS22665"
                     /old_locus_tag="pgaptmp_004533"
     CDS             1153..1890
                     /locus_tag="ACHOSM_RS22665"
                     /old_locus_tag="pgaptmp_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU4560884.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1890..3986
                     /locus_tag="ACHOSM_RS22670"
                     /old_locus_tag="pgaptmp_004534"
     CDS             1890..3986
                     /locus_tag="ACHOSM_RS22670"
                     /old_locus_tag="pgaptmp_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU4560885.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4033..4311)
                     /locus_tag="ACHOSM_RS22675"
                     /old_locus_tag="pgaptmp_004535"
     CDS             complement(4033..4311)
                     /locus_tag="ACHOSM_RS22675"
                     /old_locus_tag="pgaptmp_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560886.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4312..4455
                     /locus_tag="ACHOSM_RS22680"
                     /old_locus_tag="pgaptmp_004536"
     CDS             4312..4455
                     /locus_tag="ACHOSM_RS22680"
                     /old_locus_tag="pgaptmp_004536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560887.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            4581..4991
                     /gene="psiE"
                     /locus_tag="ACHOSM_RS22685"
                     /old_locus_tag="pgaptmp_004537"
     CDS             4581..4991
                     /gene="psiE"
                     /locus_tag="ACHOSM_RS22685"
                     /old_locus_tag="pgaptmp_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HGU4560888.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5035..6510)
                     /gene="xylE"
                     /locus_tag="ACHOSM_RS22690"
                     /old_locus_tag="pgaptmp_004538"
     CDS             complement(5035..6510)
                     /gene="xylE"
                     /locus_tag="ACHOSM_RS22690"
                     /old_locus_tag="pgaptmp_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HGU4560889.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         1701 a         1700 c         1826 g         1548 t
ORIGIN      
        1 gtaattcgca tgctttagtt gtgtatactc gatcccgccc gaaatgtttt tgggtaaatc
       61 tccattcatt caatgaaggg aaattgttat gaaaaaagtt ctgtatggca tttttgccat
      121 atctgcgctt gcggcgactt ctgcgtgggc tgcacctgta caggtgggcg aagcggcagg
      181 atcggcagca acgtcggttt cggcgggtag ttcctccgcg accagcgtca gcaccgtaag
      241 ctcggcggtg ggtgtcgcgc tcgcggcaac cggtggcggt gatggttcta ataccgggac
      301 cacaacaacc acaaccacca gtacccagta ataaagtatg tatccccaaa ataattcgag
      361 ttgcaggaag gcgacaagcg agtgagtccc cgggagcata gataactatg tgaccggggt
      421 gaacgagtgc ggtcaccgca tctgcagctt gaagtatgaa ggggattaac cataaccaca
      481 ctccggtgtg gttattctgc ccctctggag aagagtcgtg aagcgacctg cactcattct
      541 tatctgcctg ttattacagg cctgttcagc cacgactaaa gagctgggca attcactgtg
      601 ggacagtctg ttcggtacac caggcgtaca gctgacggat gatgatattc aaaatatgcc
      661 ctacgccagc cagtacatgc agcttaatgg cgggccgcag ttatttgtgg tgctggcctt
      721 cgctgaagac ggacaacaaa aatgggtcac tcaggatcag gctactctcg ttacccaaca
      781 tggccgtctg gtgaagactc tgctcggcgg cgacaacctg attgaagtga ataaccttgc
      841 tgccgacccg ctgattaaac ccgcacaaat cgttgatggc gcaagctgga cgcgcacgat
      901 gggctggacc gagtaccagc aggtacgcta cgccaccgca cgctcagtct tcaaatggga
      961 tggcaccgat accgtcaaag tcggcagcga tgaaaccccg gttcgcgtgc tggacgaaga
     1021 agtctccacc gaccaggcgc gctggcataa ccgctattgg atcgacagcg aagggcaaat
     1081 tcgccagtcg gaacagtatc tcggcgcgga ttattttccg gtgaaaacca ctctcatcaa
     1141 ggcggcaaaa caatgattaa acaaactatt gtcgcgttgc ttttgagtgt gggagcgtca
     1201 tcggtcttcg cggcaggaac cgtcaaggtg ttcagcaatg gcagcagtga ggccaaaacg
     1261 ctaacgggcg cagagcattt aatcgatctg gtaggccaac cgcggctggc aaacagctgg
     1321 tggcccggtg cggtgattag cgaagagctg gcaacggcgg cagcattgcg tcagcagcag
     1381 gcgttgctga cacggctggc agaacagggc gcagattcca gcgccgacga tgccgctgcg
     1441 attaacgcct tacgccagca aattcaggcg ttgaaagtga cgggcaggca aaaaatcaat
     1501 cttgatcccg atatcgtccg cgttgccgaa cgcggtaacc cgccgttgca gggcaactac
     1561 acgctgtggg tcggaccacc gccgtccacg gtcacgttgt tcgggcttat cagccgtcct
     1621 ggcaagcagc cattcactcc tggtcgcgac gtggcgagct atctctctga ccagagcctg
     1681 ctcagcggtg cggatcgcag ctacgcgtgg gtggtttacc cggacggacg cacgcaaaaa
     1741 gcgccggtgg cttactggaa caagcgtcac gtagagccga tgcccggcag cattatttat
     1801 gttggcctcg cggactccgt ctggagtgag acgcctgatg cccttaacgc cgacattctt
     1861 cagactctga cgcagcggat acctcaataa tgaaaaaaag acatctgctt agcttactgg
     1921 cgctgggcat tagcacagct tgctacggcg aaacatatcc tgcgcccatt ggtccgtcgc
     1981 agtcggattt cggtggcgta ggattattac aaacgcccac cgcgcgtatg gcacgggaag
     2041 gggagttgag tctgaactat cgcgataacg atcagtaccg ttattactca gcttcagtgc
     2101 aactcttccc gtggctggaa acaacgctgc gctacaccga cgtgcgcacc cggcagtaca
     2161 gcagcgtcga agcgttctct ggcgatcaaa cgtataaaga taaagccttc gatctcaaac
     2221 tgcgtttgtg ggaagagagt tactggctgc cgcaagtggc ggttggtgcg cgggatatcg
     2281 gcggtacggg gctgtttgat gcggaatatc ttgttgccag caaagcctgg gggccgttcg
     2341 attttacgct cggcctgggc tggggatatc tgggcaccag cggtaatgtg aaaaatccgc
     2401 tctgttcagc cagtgataaa tattgctatc gcgataacag ctacaaacaa gcgggatcta
     2461 tcgacggtag ccagatgttc cacggtcctg cctcactgtt tggcggcgtg gaataccaga
     2521 cgccctggca accgctgcgc ctgaaactgg agtatgaagg caataattat cagcaggatt
     2581 ttgccgggaa gctggagcaa aaaagtaagt ttaacgtcgg tgcgatttat cgcgttaccg
     2641 attgggccga cgttaacctt agctatgaac gtggcaacac ctttatgttt ggcgtcacgc
     2701 tgcgcaccaa ctttaacgat ctgcgcccgt cttacaacga taacgcccgc ccgcaatatc
     2761 aaccgcagcc gcaggatgcc attttgcagc attcggtggt ggcgaatcag ttaacgctgt
     2821 tgaaatacaa tgccggactt gccgatccac agatccaggc gaaaggcgat acgctgtatg
     2881 ttaccggcga gcaggtgaaa tatcgtgatt cgcgcgaagg gatcatccgt gctaatcgga
     2941 tcgtgatgaa cgatctgccg gatgggatca aaacgatccg cattacggaa aatcgtctta
     3001 acatgccgca ggtgacgacg gaaaccgatg tcgccagcct gaaaaatcat ctcgccggag
     3061 agccgttggg ccacgaaacg aagctggcgc aaaaacgcgt cgagccagtg gttccgcagt
     3121 ccaccgagca gggctggtat atcgacaaat cacgctttga tttccatatc gatccggtgc
     3181 tgaaccagtc ggtcggtggc ccggaaaact tttacatgta tcagctgggc gtgatgggaa
     3241 cggcagattt gtggctgacg gaccatctgc tgaccaccgg cagcctgttt gcaaatcttg
     3301 ccaacaacta cgacaagttt aactacacca atccgccgca ggactcgcac ttaccgcgcg
     3361 ttcgtaccca tgtgcgcgag tatgtgcaga acgatgtcta tgtgaataac ctgcaagcca
     3421 actacttcca gcatctgggc aacggcttct acggtcaggt ctatggaggt tatctcgaaa
     3481 ccatgtttgg cggtgcgggg gcagaagtgt tgtatcgtcc gctggacagc aactgggcgt
     3541 ttggtctgga tgccaactac gttaaacagc gcgactggcg tagtgcaaaa gatatgatga
     3601 aattcaccga ctacagcgtg aaaaccggac atctgaccgc ctactggacg ccatctttcg
     3661 cccaggacgt gttggtgaaa gccagcgtcg ggcagtatct ggcaggggat aaaggcggca
     3721 cgctggagat cgctaaacgc tttgacagcg gcgtggtggt gggtggctat gccacgatca
     3781 ccaacgtttc gaaagaggag tacggcgaag gggacttcac caaaggcgtg tatgtctcgg
     3841 taccgttgga tctcttctcg tctggcccga cacgcagccg tgcggcgatt ggctggacgc
     3901 cgctgacgcg tgacggtggt cagcaacttg ggcgtaagtt ccagttgtat gacatgacca
     3961 gcgaccgtag cgtcaatttc cgctaagtca tgggaaaggt gccagttttc gcactcactg
     4021 gcaccttcat tcttaatggc ctgtcgagcc atcaggaacg cagttcaccc ccggtttgcg
     4081 atctggtgtg taaaggcgaa aacattctgt cttcctgtcc gtcgatatcg gttgcaggtc
     4141 tggttgcctg agcgactggg ccatcattgg gctggagaca ttgctttgcg gtaggtcggc
     4201 tttatcagca gtaccgagcg gaccagcata agcaggaaga acagagactg ataacatcaa
     4261 agcagcaaaa taaggcttca tttttaccac ctttatcagg ttacgtttca tttgttccag
     4321 aggaacattg tcgatttttc gcgcattgct ggtggctggg aatcacctga atgggtgatt
     4381 tttgaattac cggcttgggt gcggtttgtc ttgccggatg cgccgccagg cgcggcttat
     4441 ccggcctacg ggtaggtata tccggctttg gtggaggcgc gctccaaatc caggttgaac
     4501 aaaacataca caaaaaatat agatctccgt cacatttttg cgttatacag gaagctcgcc
     4561 actgtgaagg aggtactgct atgacgtcac tctctcgtcc gcgcgtggag tttatctcca
     4621 ccattttgca gaccgtactc aatcttggcc tgctgtgcct cggcctgatt ttggttgtct
     4681 tcctcggcaa agaaacggtg catctggcag atgtgctgtt cgcgccagaa caaaccagca
     4741 aatatgagct ggtagaagga ctggtggttt actttctcta tttcgaattt atcgcgctga
     4801 ttgtgaagta ctttcagtcc ggttttcact tcccgctacg ttactttgtc tatattggga
     4861 tcaccgccat tgtgcgcttg atcatcgtcg atcataaatc gccactggat gtgttgatct
     4921 actcggctgc gatcctgtta ctggtgatca ctctgtggct gtgtaattcg aaacggctga
     4981 agcgggagta aaaagacagc acgccgaact ggcgcggcgt gctggacagg aagattacag
     5041 cgtagcagtt tgttgtgttt tcttcgtttc cggttcccag agggcttcca gctcctcaag
     5101 ggttttacct ttggtttccg ggacaaattt ccacataaat agtgctgcca gaacgcccat
     5161 acacccgtaa atccagtagg agaaaccgtt gtggaaatgg gccaccagcc aggagttttt
     5221 gtccatcatc gggaaggtcc aggagacgaa gtagttcgcc agccactggg ccgccaccgc
     5281 gattgccagc gctttaccac gaatagcatt cgggaagatt tccgacagca gtacccagca
     5341 taccggaccc caggacatgg caaaggcagc aacatagaac agcatcgaca gtagcgccac
     5401 aatacccggt gcctgagtgt aaaacgcggt accgaggcta aacataccga ttgccattcc
     5461 gagtgcgccg ataatttgca gtggcttacg accaaattta tccaccgtca taattgccag
     5521 aacggtgaag gtgaggttga taactccgac aataatggtc tgcaacagcg cgatatccgt
     5581 gctggccccc agcgttttga acacttccgg cgcgtagtac agcaccacat tgatgccgac
     5641 aaattgttgg aagatggaga gcataacgcc gatgacaatc acgcccacgc caaacatcag
     5701 cagacgacca ccggttttgc ggccatgatc cagggagtgt ttaatttcct gtactgcctg
     5761 agttgcaagc gtgttgccca taattttgcg caggatactt tccgcctgtt cttgcttgcc
     5821 gcgtgacatc agccagcgag gactttctgg cacggtatac agcagcatta agaacagcag
     5881 tgcagggata cattccgagg caaacatata acgccagccg tcagtattca gccagctggc
     5941 atcaccggaa cgggcaataa aatagtttac gcagtaaact aaaagttgcc cgaaaataat
     6001 cgcaaactgg ttaaatgaaa ccagttttcc gcgaatatga gctggagcca gttccgcaat
     6061 atacattggt gaaagcattg aggctaaacc aacgccaata ccgccaataa tacgataaat
     6121 aacaaattcc gggacataac ctgccagata aataggtact gtgttgtccg ggtttataga
     6181 ggtaaaacca agttctggcc aggcagaacc tacaccagaa ataaaaaaca ggacagcagc
     6241 aatcttaagt gaatcacgac ggccgaagcg gttactgcaa taaccaccga gggcaccgcc
     6301 gatgatgcaa ccaatcagag cgctggctac gcaaaatcct aacagggagt tggcagcgga
     6361 ttcacttaag ttttgtggag caacaaagac ggtattgagt gactcaacag taccggaaat
     6421 aacggcggtg tcgtagccaa ataataaacc acctaatgta gcgactaagg taatcgaaaa
     6481 tatataactg gaattatact gggtattcat tcagacctgc cttagaccat tctgatcttt
     6541 tatcagtgca ttgatgttgg aattgtcagg cagttaaggc aattatcatt ttttacaatg
     6601 cgtttacgta atgtcttttc tgtgatctta attgtgataa ttatcaaaaa ttgatgaaaa
     6661 caatactatt gccgtgactc agagcacgaa agagaattat cgtaagtggg aaaacaaata
     6721 acgtaaaaat aataagctct atgatgggaa tattaaccgg cgaacgattc agatt
//
LOCUS       DAZKHC020000176         6416 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZKHC020000176 DAZKHC020000000
VERSION     DAZKHC020000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.176"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..445
                     /locus_tag="ACHOSM_RS22695"
                     /old_locus_tag="pgaptmp_004539"
     CDS             <1..445
                     /locus_tag="ACHOSM_RS22695"
                     /old_locus_tag="pgaptmp_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HGU4560890.1"
                     /translation="DLVLKALRKAGLYSNATLTDADPQAIEDAINDLEDMMAAWQAKG
                     IELGYQFADTENGIMPLPDDDSGIPAWANDGVALKLAVQVCMDNVIQPSDALLTAADS
                     AYQTICIALTKIPPLERRNDMPRGSGNKSAFTWNRFYIEKDDPST"
     gene            455..1873
                     /locus_tag="ACHOSM_RS22700"
                     /old_locus_tag="pgaptmp_004540"
     CDS             455..1873
                     /locus_tag="ACHOSM_RS22700"
                     /old_locus_tag="pgaptmp_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HGU4560891.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKQYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARCF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKKVGRIRKNI
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            1873..2721
                     /locus_tag="ACHOSM_RS22705"
                     /old_locus_tag="pgaptmp_004541"
     CDS             1873..2721
                     /locus_tag="ACHOSM_RS22705"
                     /old_locus_tag="pgaptmp_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HGU4560892.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHESRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSSNSATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            2721..3200
                     /locus_tag="ACHOSM_RS22710"
                     /old_locus_tag="pgaptmp_004542"
     CDS             2721..3200
                     /locus_tag="ACHOSM_RS22710"
                     /old_locus_tag="pgaptmp_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HGU4560893.1"
                     /translation="MEIKLIDNPVKLAEFLNNPANTGNIVDSGDKYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYTSNGKPVGITIYQLPRSKYEELKNVNFPDCQ"
     gene            3175..3849
                     /locus_tag="ACHOSM_RS22715"
                     /old_locus_tag="pgaptmp_004543"
     CDS             3175..3849
                     /locus_tag="ACHOSM_RS22715"
                     /old_locus_tag="pgaptmp_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HGU4560894.1"
                     /translation="MLIFQIANKHLSKAVYCKGGSDGGSKAQARATEKGIELQREMWQ
                     TNMQNLAPFTPLAQQYVSQLQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAAAE
                     ATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYANNV
                     SQLYQQQAAASAANANRPSGLQSALGGAMSGAASGAMIGSVVPGIGTAVGAIGGGIIG
                     GLGSLF"
     gene            3859..5160
                     /locus_tag="ACHOSM_RS22720"
                     /old_locus_tag="pgaptmp_004544"
     CDS             3859..5160
                     /locus_tag="ACHOSM_RS22720"
                     /old_locus_tag="pgaptmp_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HGU4560895.1"
                     /translation="MATWQQGINSGGFLAGIGGQNSNAPKASDVSEALAYIRQNNEME
                     RSGRNNIGLQALQGLGSVAQTYQAAKQQEADAAFQKEYAAAIQSGDRQQVRDLMTKYP
                     GQLEKIQSGMKWADEDQRNSIGTLAAGARLAASSPEAMQSWLQNNAKELARVGVDPNN
                     VAQMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEA
                     LTARGQDIQIRGQNISAQNAALSREIQRAELQEKALDRQIARESNQLKLEELKQKQAD
                     VRQKADIARADRQAAAQGAVDTFSTALDSLNEIEQSPGLSKAVGIRSAFPTVPGSDAA
                     NFEARLDTFKAQTFLPMVQSLKGMGALSDAEGKKLSDAVGALSPKMSEKAFRDSIGKI
                     RNQLESKLSTVKKQFDYQEPVQNMPGQQSTTGSNFSSLWGD"
     gene            5160..>6416
                     /locus_tag="ACHOSM_RS22725"
                     /old_locus_tag="pgaptmp_004545"
     CDS             5160..>6416
                     /locus_tag="ACHOSM_RS22725"
                     /old_locus_tag="pgaptmp_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HGU4560896.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGENAEQ
                     AKQAFYAAYPLPSVQPVETQQPVAQQQPQQSGFMSDLGEAVKETGRGLVQAGVNVANI
                     PASVADAVTSAAAWAGGKLGIGDGTYQPAPRVTTQGLEQDFGLQQGALTPQTTEGRVF
                     AEALPYLTPAGVERAAAQAPTLAGRIAQGATRLLAENAVGSLAANSAKDDAEALATDL
                     GVGVLAGGAINAAGRGLGAAYRGVRGSIAPEAQQAIRFAEREGVPLHTTDLLQPTSRV
                     GKMAQTTAENIPLAGTSGMRATQQEARSQLVQKFADKFGEYDPAVVIDSLKAKTSGIR
                     RAAGNRLEQVQNAMAGVNIQPARAIQQIDTEISNLQKLGKVADNETISKLQSYRDELV
                     RNAGPDGPVNLDLKQLSDLRSQFRMDV"
BASE COUNT         1733 a         1537 c         1689 g         1457 t
ORIGIN      
        1 tgatctcgtt ctaaaggctt tacgaaaagc cgggctgtat tccaatgcca cgttgacaga
       61 tgctgaccct caggcaattg aagatgccat taatgacctc gaagacatga tggcagcatg
      121 gcaggctaaa ggtatcgagc ttgggtatca gtttgctgat acagaaaacg gcatcatgcc
      181 gttaccggac gatgattcag gtatccctgc atgggcaaat gatggcgtcg ctttgaaact
      241 cgctgtgcaa gtgtgcatgg ataacgtcat tcagccgtca gacgctctcc ttaccgctgc
      301 tgacagtgca tatcagacaa tctgcatcgc tttaaccaaa ataccaccac ttgagcggcg
      361 aaatgacatg cctcgcggta gtggtaacaa aagcgcgttt acgtggaatc ggttttacat
      421 cgagaaagat gatccgagta cgtgaggtga ataaatgccg attcagcaac ttccgcttat
      481 gaaaggtgtc ggcaaagatt tcagaaacgc cgactatatc gactatctgc cagtgaatat
      541 gttggctaca cccaaagaaa tcctgaacag cagcggatat cttcgctcat tcccgggcat
      601 tgccaaacgt tctgatgtga acggtgtatc gcgcggagtc gagtacaaca tggcgcagaa
      661 tgctgtatat cgcgtgtgtg gcggcaagca gtataagggc gaaagcgagg tcggtgatgt
      721 tgccggaagt ggtcgcgtat caatggcaca cggtaggaca tcacaggcag taggcgttaa
      781 tggtcaactg gtcgagtatc gctatgatgg cacggttaaa accgtctcaa actggcctac
      841 agacagcgga ttcacacagt acgagttagg ttcagttcgc gacattacgc gcttacgtgg
      901 gcgttatgcg tggtcaaaag acggaactga ttcatggttt atcactgacc ttgaagacga
      961 atcgcatcct gaccgttaca gcgcacaata tcgcgcagaa tcgcagccgg acggcatcat
     1021 cggtatcggc acatggcgag acttcatcgt ctgctttggt tcatcaacga ttgaatattt
     1081 ctccctgact ggtgcaacca ccgttggtgc cgctttgtat gtcgcacaac catcgctgat
     1141 ggtgcagaaa ggcattgccg gaacttactg caaaacgcca ttcgctgatt cgtatgcgtt
     1201 tatcagcaat ccggcaacgg gtgcgccgtc tgtatacatc atcggatcag gtcaggtatc
     1261 accaatcgcc agcgcgagca ttgagaaaat tcttcgctcc tacactgctg atgaactggc
     1321 tgatggtgtg atggaatcgc tgcgatttga tgcgcatgaa ctgctgatta tccaccttcc
     1381 gcgccacgtc ctcgtgtacg acgcatcttc aagcgccaat ggtccgcaat ggtgtgtgtt
     1441 gaaaacaggc ctgtatgacg atgtgtaccg cgctatcgac ttcatttatg aaggcaatca
     1501 gataacgtgc ggcgataagc tggagtccgt gaccgggaaa ttgcagttcg atatcagcag
     1561 ccagtacgac aagcaacagg aacacctgct gtttactcca ctgttcaaag cggataacgc
     1621 cagatgcttc gatctggagg tggaatcatc cactggcgta gctcagtacg ccgaccgcct
     1681 gttcctctct gcaaccactg acggcatcaa ttacgggcgt gagcagatga ttgagcagaa
     1741 tgaaccgttc gtttacgaca aacgcgtttt gtggaagaaa gtagggcgca tcaggaaaaa
     1801 cattggcttc aaattgcgcg ttatcacgaa gtcacctgtc actctgtctg gttgccagat
     1861 aaggattgag taatggcgga ttcgaatctc aatgtgccgg taatcattca ggctacacgg
     1921 ctcgacacat cagtccttcc acgcaatatc ttctcgcagt cgtatctgct ttacgttatc
     1981 gcacagggca ctgatgttgg taacgtggct aacaaggcca acgaggccgg acagggcgct
     2041 tatgatgcac aggtcaggaa cgatgagcag gatgtgattc tcgctgacca tgagcagcga
     2101 atttctgctg cggaagcaac gcttgttaat catgaggagc gaatcagcca ggcagaatca
     2161 actcttcagg accatgaaac acgaatagct cagaatgaaa gcgatatttc gtcgcttgac
     2221 acaagagttc agtcgctgga gtcgcaggtt tcagaccatg aatcgcgcat tgatgctctg
     2281 gagtatgcca ctactcgcaa gaagtcagag gttgtttact ctggcgtatc tgtaaccatc
     2341 ccgacagcgc cgaccaacct tgttagcctg cttaaaacgc taacgccgtc atccggcacg
     2401 ttggcaccat tcttcgacac cgttaacaac aagatggttg tgttcaacga gaacaaaacc
     2461 ttgttcttca agctgtcgat cgtcggtacg tggcccagtg gaaccgccaa caggtcaatg
     2521 caactaacat tttccggttc tgttcctgac acactggtaa gcagtagcaa ctcggcgaca
     2581 acaaccgata acatcctgtt agctacgttc ttcagtgtgg ataaagacgg ctttcttgcc
     2641 acaaatggca gcacattaac cattcagtca aatggtgcgg cgtttactgc cacaaccatc
     2701 aaaatcattg cggagcagtg atggaaataa agctcatcga taatccggtg aagcttgcag
     2761 aattcctcaa caacccggca aacacgggaa atatcgtaga cagtggagat aaatactaca
     2821 tcaagcctga tgcggtatac ctcggcatct acgaaggatt agtgctggct ggcgttcatg
     2881 aagtgcgtaa cttctggcat agcgttgttg aatgccatgc ggtgtacgac cccggattcc
     2941 gtggtgaata tgcactgcaa gggcatcgat tattctgcaa atggcttctc gaaaactcac
     3001 cattccttaa cagcatcacc atggttcctg acacaacgaa atacggacgg gcaattatcc
     3061 gtttgcttgg cgctacccgt gttggtcacc ttgatgatgc ttataccagc aatggaaagc
     3121 ctgtaggcat cacgatttat cagttaccgc gctcaaaata cgaggagcta aagaatgtta
     3181 attttccaga ttgccaataa gcacctaagc aaagctgttt actgcaaagg tggcagtgat
     3241 ggcggttcaa aagcccaggc acgcgcaact gaaaaaggta tcgaactgca gcgtgaaatg
     3301 tggcagacga acatgcagaa ccttgcaccg ttcacgccac tcgctcagca gtacgtatca
     3361 cagttgcaga atctttcctc tcttcagggg caaggtcagg cgcttaacca gtattacaac
     3421 tctcagcagt ataaagacct tgcaggtcag gcgcgctatc agagtctggc agcagcagag
     3481 gcaacgggtg gattaggctc tacagcaacc ggaaaccagc tggcagcaat cgcgcctaca
     3541 ctcggtcaaa actggctgtc aggtcagatg aataactaca acaatctggc aaatatcggc
     3601 cttggcgctc ttacaggtca ggcaaacgcc ggacagaact acgctaacaa cgtcagccaa
     3661 ttgtatcaac agcaggcggc agcatctgcg gcgaatgcta accgaccatc aggattgcaa
     3721 tcagctttgg gcggtgccat gagcggtgcg gcatcagggg cgatgattgg ctctgtggtg
     3781 cctggaatag gtacagctgt tggcgctatt ggtggcggta ttatcggtgg tcttggatca
     3841 ttgttttaag gtgggaatat ggctacttgg caacaaggaa tcaactcagg cggttttctt
     3901 gctggtatcg gtgggcaaaa ctcaaatgcg ccaaaggcaa gtgatgtaag tgaggcgttg
     3961 gcctatattc gccagaacaa cgaaatggag cgttcaggtc gcaataacat cggccttcag
     4021 gcgttgcagg gacttggtag tgtcgctcaa acatatcaag ctgcaaagca acaggaagcg
     4081 gatgctgcat tccaaaaaga atatgcggca gccatccagt ccggtgatcg acagcaggtt
     4141 cgagatctga tgaccaaata tcctggtcaa ttagagaaga ttcagtctgg tatgaagtgg
     4201 gcagacgaag accagcgcaa ttctatcggc accttagcgg ctggcgcacg ccttgcggcc
     4261 tcgtctccag aagcaatgca atcatggctg caaaacaacg ccaaggaact ggcgcgcgtc
     4321 ggtgttgacc ctaacaacgt tgctcagatg tatcagcaga atccctcagg atttggtgag
     4381 tttgttgatc accttgggat ggctgctctt ggtccgattg attacttcaa tgttcaggac
     4441 aagatggctg gtcgtgagat tgaccgaggc aggctggcag agacaatccg cagcaatcag
     4501 gctggagaag cactaacagc gcgtggtcag gacatccaga tacgtggaca gaacatcagc
     4561 gcacagaatg ctgctctttc ccgcgaaata caaagagcag aattacaaga aaaggctctg
     4621 gacagacaga tagccagaga aagcaatcag ttaaagcttg aagagctaaa acagaaacag
     4681 gcagatgttc ggcaaaaggc tgacatagcc cgcgctgaca ggcaggccgc cgctcagggt
     4741 gcagttgata cgttcagcac cgcgcttgat tctctcaacg agatagagca aagccccggc
     4801 ctttcaaaag cagtaggaat tcgctcagcg tttccgacag ttcctggctc tgatgcggct
     4861 aactttgaag caaggctcga cacctttaaa gctcaaacat tccttcctat ggtgcagtcc
     4921 ctgaagggta tgggcgctct ttcagatgct gagggtaaaa aattatccga tgcggttggt
     4981 gccctaagcc ccaaaatgag tgaaaaggct tttcgtgact ctatcggaaa gattagaaat
     5041 cagcttgaaa gcaagttgag cactgttaaa aaacagtttg attatcagga gccggttcag
     5101 aatatgccag ggcaacaatc tactactggc agtaactttt cttcactatg gggtgattaa
     5161 tggctaaagc atggaaagat gttatcgcct ctccacagta tcaggcgtta gcaccagaac
     5221 aaaaagcgca ggctcaggag caatacttca atgaagtcgt tgccccgcaa gccggagaaa
     5281 atgcagagca ggctaagcaa gctttctatg ctgcctatcc attgccatct gtgcagccag
     5341 tggagacaca acaaccagta gcacagcaac aaccacagca aagtggattt atgtctgatc
     5401 ttggcgaagc agtaaaagag actggtcgcg gactggtgca ggctggcgtg aacgtggcaa
     5461 atatacctgc atcagttgcc gatgctgtaa cgagcgcggc ggcttgggct ggcggtaaac
     5521 tcggcattgg cgatgggaca tatcaaccag cgccacgagt aacaacgcag ggattagagc
     5581 aggactttgg ccttcagcaa ggtgcgctga ctccacaaac gacagaggga agggtatttg
     5641 ctgaagcatt gccttacctc actcctgctg gcgttgagag agcggcagca caggcaccaa
     5701 cacttgctgg tcgaattgct cagggggcaa ctcgccttct cgctgaaaac gcagttggat
     5761 cacttgctgc aaatagtgcg aaagatgatg cggaagcact cgccaccgat ttaggcgttg
     5821 gtgtgctggc tggcggtgct attaacgctg ccggacgtgg attaggtgct gcttatcgtg
     5881 gtgttcgtgg ttcgatagca ccagaagcgc aacaggctat cagatttgca gagcgtgaag
     5941 gagttcctct gcacaccaca gacctgttac aaccaacttc ccgcgtcgga aaaatggcgc
     6001 agactacagc agaaaatatc cctctggctg gcacaagcgg aatgagagca acgcaacagg
     6061 aagcgagaag ccagttggtg cagaaatttg ctgataaatt cggtgagtat gatccagcgg
     6121 ttgttattga cagccttaaa gcgaaaacat caggaattcg tcgtgccgcc ggtaatcgac
     6181 tggagcaggt tcagaatgct atggctggag taaacattca gcctgcgcga gcaattcagc
     6241 agattgatac agaaatatct aacctgcaga agcttggtaa ggttgctgat aacgagacta
     6301 tttcaaaact tcagtcatat cgtgatgagc ttgttcgcaa tgctggtcct gatggtccgg
     6361 taaatctgga tttgaagcaa ttaagcgacc tgcgcagcca gttcagaatg gacgtg
//
LOCUS       DAZKHC020000177         6267 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZKHC020000177 DAZKHC020000000
VERSION     DAZKHC020000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.177"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..237
                     /locus_tag="ACHOSM_RS22730"
                     /old_locus_tag="pgaptmp_004546"
     CDS             <1..237
                     /locus_tag="ACHOSM_RS22730"
                     /old_locus_tag="pgaptmp_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4560897.1"
                     /translation="VQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELSLRL
                     SPSVSTHTEKREDPYFKASYDNVDYSQIPTGFRG"
     gene            234..728
                     /locus_tag="ACHOSM_RS22735"
                     /old_locus_tag="pgaptmp_004547"
     CDS             234..728
                     /locus_tag="ACHOSM_RS22735"
                     /old_locus_tag="pgaptmp_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4560898.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQSVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLFRRAATVWMAAFRESHSQPERNNFLAHRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            728..1381
                     /locus_tag="ACHOSM_RS22740"
                     /old_locus_tag="pgaptmp_004548"
     CDS             728..1381
                     /locus_tag="ACHOSM_RS22740"
                     /old_locus_tag="pgaptmp_004548"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU4560899.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            1378..1704
                     /locus_tag="ACHOSM_RS22745"
                     /old_locus_tag="pgaptmp_004549"
     CDS             1378..1704
                     /locus_tag="ACHOSM_RS22745"
                     /old_locus_tag="pgaptmp_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HGU4560900.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTVVKDDDSEAVGIIRSLLAGEENARLRAAHWLH
                     ERGLKV"
     gene            1701..2096
                     /locus_tag="ACHOSM_RS22750"
                     /old_locus_tag="pgaptmp_004550"
     CDS             1701..2096
                     /locus_tag="ACHOSM_RS22750"
                     /old_locus_tag="pgaptmp_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4560901.1"
                     /translation="MKLLLPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKASCKLATR"
     gene            2259..3074
                     /locus_tag="ACHOSM_RS22755"
                     /old_locus_tag="pgaptmp_004551"
     CDS             2259..3074
                     /locus_tag="ACHOSM_RS22755"
                     /old_locus_tag="pgaptmp_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGU4560902.1"
                     /translation="MNQLLVIDGVSVRQYFESNYCLNDLQKAALLAAGENRSSRSLEV
                     HEFMRRPETKALVELLEEETTGDSRSIPVITIQGRNGGTYVCKELVYAYAMWISPAFS
                     LKVIRTFDALHNSSPEETTSDKIKSGVILLESAAKTLNLSNSSKLGAYQKLSKVAGLP
                     ELMPIYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYS
                     RTAINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            3082..4071
                     /locus_tag="ACHOSM_RS22760"
                     /old_locus_tag="pgaptmp_004552"
     CDS             3082..4071
                     /locus_tag="ACHOSM_RS22760"
                     /old_locus_tag="pgaptmp_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4560903.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRHRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            4085..4837
                     /locus_tag="ACHOSM_RS22765"
                     /old_locus_tag="pgaptmp_004553"
     CDS             4085..4837
                     /locus_tag="ACHOSM_RS22765"
                     /old_locus_tag="pgaptmp_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4560904.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMATQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKICGRCNGKRFSRLPTTLARHHVQKLVPDLSDYQ
                     WYKGYANVIDKLVTKCWQEESYAEAQLRKVTR"
     gene            complement(5116..5205)
                     /locus_tag="ACHOSM_RS22770"
                     /old_locus_tag="pgaptmp_004554"
     CDS             complement(5116..5205)
                     /locus_tag="ACHOSM_RS22770"
                     /old_locus_tag="pgaptmp_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560905.1"
                     /translation="MNKPVCLDDWLIGFKSLCCTLTLIALLIM"
     gene            complement(5260..5472)
                     /gene="cspF"
                     /locus_tag="ACHOSM_RS22775"
                     /old_locus_tag="pgaptmp_004555"
     CDS             complement(5260..5472)
                     /gene="cspF"
                     /locus_tag="ACHOSM_RS22775"
                     /old_locus_tag="pgaptmp_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HGU4560906.1"
                     /translation="MSRKMTGILKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            5773..5988
                     /gene="cspB"
                     /locus_tag="ACHOSM_RS22780"
                     /old_locus_tag="pgaptmp_004556"
     CDS             5773..5988
                     /gene="cspB"
                     /locus_tag="ACHOSM_RS22780"
                     /old_locus_tag="pgaptmp_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HGU4560907.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSVESGAKGPAAANVIITD"
BASE COUNT         1632 a         1367 c         1691 g         1577 t
ORIGIN      
        1 gtgcaggatg gtcgtactca caaacaaatc tgcgagatgt acagccgcgt cagccgcgat
       61 ccgttctggt gccgtaacgt gctcagcccg tcgaagttgc gggaaaaatg ggatgagctt
      121 tccctgcgct tatcgccgtc cgtcagcacg cacacagaaa aacgtgaaga cccgtacttc
      181 aaagccagtt acgacaacgt ggactacagc cagatcccga caggattcag ggggtgatca
      241 tgagtctttt gaatgacgtt cagaaattca ttgaagccca tccgggctgt acttccggag
      301 acattgcaga tgcttttgct ggttactcac ggcagcgcgt tctgcagtca gcaagcaagt
      361 tacgtcagag tgggcgtgtg gctcaccgtt gtgaaggaga tacacgcaga catttcccgc
      421 gcctgactga gagagcgcag gagccggaac cacaatctgt tcgtgaaacc agacctgtgc
      481 gcaatttcta tgtcggcact aacgaccccc gggtgatttt gtgcctgacc cgccaggctg
      541 aagaactgga gtccaggggc ttattccgtc gagctgcaac ggtgtggatg gcggcattcc
      601 gtgaaagcca ctcccagcca gaacgaaaca attttctggc gcatcgtgag cggtgcttac
      661 ggaaaagcag caagcgcgct gcatcgggtg aagagtggta tctgtcaggg aattacgtgg
      721 gggcttaatg agtaataaat attgccaggc gctggtggag ctgcggaaca aaccagccca
      781 tgaactgaag gaagtgggcg atcagtggcg cacgccggac aacattttct ggggaattaa
      841 caccctgttt ggcccgtttg ttctggatct gtttactgat ggtgataacg ccaaatgtgc
      901 cgcttattac actgcggaag acaacgcgct ggcgcatgac tggtcagaac gtcttgcgga
      961 gcttaaaggt gctgcctttg gtaatccccc gtacagccgc gccagtcagc atgaggggca
     1021 atacatcacc ggcatgcgtt acatcatgaa acatgccagt gctatgcgtg ataaaggcgg
     1081 gcgctatgtt ttcctgatca aagctgccac cagcgaagtg tggtggccgg aagatgcaga
     1141 ccatattgct tttattcgcg ggcgtattgg ttttgaactg cctgcctggt ttatcccgaa
     1201 ggatgagaag caggtgccga caggcgcttt cttcgctggt gctattgctg ttttcgacaa
     1261 gacctggaag ggaccggcaa tcagctacat cgggcgcgat gaacttgagg catgtggtga
     1321 ggcgtttctg gcgcaggttc gccagcaggc ggaaaaactg gtcagggaga tggcggcatg
     1381 acgacgttaa ctcaatgcca gcagcaggtg ctggatatgc tgatttctta tcagaaagaa
     1441 cgtggcttcc cgccaaccaa tcaggaggtg gcaaccatgc tgggataccg ttcagtgaat
     1501 gcagcggtgg agcatcttcg cgcactggag aaaaaaggcg tcatcacgat aaagcgtggt
     1561 gtggcccggg ggatcacgct tcataccgtg gtgaaggacg acgacagcga ggcggtcggg
     1621 attatccgct cactgcttgc cggtgaggaa aacgccaggc tgcgtgcagc ccattggtta
     1681 catgagagag gcctgaaagt atgaagctgc tcctgccttt tccgcccagc gtgaacacgt
     1741 actggcgaca ccccaacaaa ggggcgtttg ctggtaagag cctgataagc gcggcggggc
     1801 gaaaattcca gagcgcggcg tgtgcagcaa tagttgagca gttacgtcgt ctgccgaaac
     1861 caacgtcggc acctgcttca gtggagatcg tgttgtttcc tccggataac cggatccgcg
     1921 atctggacaa ctataacaag gcgctgtttg acgccctgac ccacgcgggt gtgtgggaag
     1981 acgacagtca ggtgaaaaga atgctggtgg agtggggacc ggttatcccg gaggggaagg
     2041 tcgagatcac tatcagtaag tacgaaaaag cgagttgcaa attagcaact cggtaacgga
     2101 attaagcaac accctaaatt tgggtattac ctcgttaaag atactgtatt tatgaacagt
     2161 gtatccttga taactattaa aaatcgcagt aagttcatcc ggcatcaacg aaaagggagt
     2221 gcagtcccgc tcgtggataa aaatttgtgg agaaaccaat gaatcagttg cttgtaattg
     2281 atggcgtttc tgtgcgccag tacttcgaat ctaactactg tcttaacgac cttcagaaag
     2341 ctgctcttct tgccgctggt gagaatcgct cctcccgttc gctggaagtt cacgagttta
     2401 tgcgtcgtcc tgaaacgaag gctcttgtgg aattattgga agaagaaact acgggagatt
     2461 cccgtagtat tcctgtcatc accattcagg ggcgcaatgg tgggacgtat gtctgtaaag
     2521 agctggtcta tgcatatgca atgtggatca gcccggcatt cagcttaaaa gtgatacgta
     2581 cttttgatgc gcttcataat tcatcaccag aagaaaccac atccgacaaa attaaatccg
     2641 gggtcattct gcttgaatca gcagcaaaga ctctaaatct gtcaaactcc tcgaaacttg
     2701 gtgcatacca gaaattatca aaggtagctg gtcttcctga acttatgccg atctatgcca
     2761 ttgatgcacc tgctgatgcg ccagatggtt caagccgccc tacgctgtcg ctgagtgcac
     2821 tgctgaagca gtatggtatc cgcctgacgg ctaatcaggc atatcaccag atggcgaagc
     2881 tggggatcgt tgaacaacgc gaacgataca gtcgtaccgc gattaacaac atcaaaaaat
     2941 tctggtcgct gaccgcgaaa ggctgcatgt tcggcaagaa catcaccagt cctgcaaatc
     3001 cgcgcgagac gcagccgcat ttcttcgaat cccgattccc tgagctgtta aagctgctcg
     3061 ataccgttca ttgaggtgac cgtgagagca ctactgaccc ctgaaattgc cccgcgtatg
     3121 gggatcgtat tgttcagacc aggttcagag ctgatgccct tgtttatgca ggggcgtgtc
     3181 ctgctggagc ctgagccgga acgttattca tctttcgcca gtggtgccgt tccggcggca
     3241 tcacaaccgc tggcggatga tcctgccgtt cgggccgtgt tccgcaatga ggcagtgatc
     3301 cgtcgtgctg gtggcgtgga atgtcttgaa agctggttac ttcgtgaaaa aggctgccag
     3361 tggcctcatt ccgactggca cagcgagaac atgacaacaa tgcgacacgc tccgggcgca
     3421 atccgtctgt gctggcactg cgataaccag ctgcgcgatc agttcacgga acggctggaa
     3481 tcaatggcaa cggataactg tgcccgctgg gtgttgtctg ttgtgcgtcg ggatctcggt
     3541 tttgatgaca gtcatgttgt gacaatgccg gaactgtgct ggtggctgat tcgtaatgat
     3601 ctggcggatg ccttaccgga aagtgcagcc cgtaaggcac tgagattacc gaagcctgtt
     3661 gtgccgtctg ttacccggga aagtgacctt gtgccttcgg ttcctgccac cagcatcatc
     3721 caggataagg cgaaaaaggt gctggcgctg aaagtggatc cggagtcgcc ggagtctttt
     3781 atgttacgcc caaaacgtca ccgctgggtt aatgaaaagt acacgcgctg ggttaagacg
     3841 cagccgtgcg catgttgtgg aaaacctgct gatgatcccc accacctgat aggccacggt
     3901 caggggggaa tgggtacaaa agcgcatgac ctttttgtgt tgcctttgtg cagaaagcat
     3961 cacgacgagc tgcatgcgga taccgtggca tttgaagaga agtatggctc ccagctggag
     4021 ctgatatttc gttttatcga tcgtgcgctg gcaataggcg tactggcgta agtggagaac
     4081 gagcatgaac cttgaagcct taccaaaata ttactcccca aaatctccaa aattgagtga
     4141 tgacgcaccg gcgacaggct ctggtggttt aacgattacg gatgtgatgg ctacgcaggg
     4201 gatggtgcag tcgaaagcac cgcttgggtt tgccttattc ctggcaaaag ttggtgttca
     4261 ggatcctcag tttgcgattg aaggtctgct caattacgcg atggcactgg ataacccgac
     4321 attgaataaa ttgagtgaag aaacccggct acagattatt ccttaccttg tgaattttgc
     4381 ctttgctgat tattccaggt ctgcggcaag taaggctcgt tgtgagcatt gtgctggtac
     4441 tggatttcat aatgtattgc gcgaagtggt gaaacactcc agaagcgggg aatctgttat
     4501 caaagaagag tgggtgaagg aactatgtca gcattgccat ggtaagggag aagtcagcac
     4561 agcgtgcaga gggtgtaagg gtaaaggtat tgtcttggat gaaaaaagga cccggcttca
     4621 tggcacacct gtttataaga tttgtgggcg ttgcaatgga aaacggttta gtcgtttacc
     4681 aaccacactg gcgcggcatc atgtccagaa gctggtaccg gacctgagtg attatcagtg
     4741 gtataaagga tatgcaaatg tcattgataa actggttaca aagtgctggc aggaggaatc
     4801 ttacgctgaa gcacaattga gaaaggtgac gagataagtg attttcgccg aagatggcga
     4861 cgtgatgctt gcatttttca aaaaatatgg ataaaatttt ttcaacgatg ggctttgtat
     4921 acccgaagtt aagaaaaagt agaaaacccg ctgatgagcg ggtttttgtg ctttaaatag
     4981 ggtaatagag atgttgaatc tcattacggg attcatgtta gtttacttat tatttatcgg
     5041 gtgactttgt tttttgcctg atgtttaaaa tgctttcttc cagtacaatg tccatagata
     5101 caatgagtct gcttattaca ttattagcag agctattaag gtcaaagtac agcataagct
     5161 tttaaagcca atcaaccagt catcaagaca gacgggttta ttcatgaaaa ctctccatgt
     5221 ttgattcgat ggggcttgaa gttaaagctt taatatagct catgaaaggt aaacattggc
     5281 agctgatggt ccacgcagac catttatccg gcaaaattcc acgcgtaatc cggtggtaat
     5341 ttcttctgca tcgcggagat tgagcgctga aacatgaagc tggacatcga tacgaccatc
     5401 ggatggggtt ataagacctt tgccgctttt gccgtcaaag gttttgagaa ttcctgtcat
     5461 tttacgggac aaaaaaattc cttaatactg ataacttggc gcactataca cacgttcctg
     5521 aagaaagcta tagttttttg atggggttga agatggctgg atgtctaaaa taaacattgc
     5581 ttcatatgtt caactatgcg ttaatgattg cgtcggtttg aagaacagac gatatacgaa
     5641 gtagtttact aaagcagttc tcatttcagg tgttattcac ttattccttc tttgagtctc
     5701 tccaattaag tacgaagtcg tttctgttat acaagccatt tatgccgaaa ggctcaagtt
     5761 aaggaatgta gaatgtcaaa taaaatgact ggtttagtaa aatggtttaa cgctgataaa
     5821 ggttttggct ttatttctcc tgttgatggt agtaaagatg tgtttgtgca tttttctgcg
     5881 attcagaatg ataattatcg aaccttattt gaaggtcaaa aggttacctt ctctgtagag
     5941 agtggtgcta aaggtcctgc agcagcaaat gtcataatta ctgattaaaa ttcatagttt
     6001 gtctgtatac gataacgaag aaggctgatg cctgagtgga gatacagaca gagtggtgaa
     6061 tattggatct ctttaataaa gagtaaggag gtccaataca tgaaacaatg gccagcatat
     6121 ttggcaataa cttaatcagg aaaagtatgc taaccattgt ggtgaagtgc aggtttgctg
     6181 catgaatagt tttacagcag aagctaactg ctggcatagc aaaacaaagt gcgtaagtgg
     6241 atgactccca caaaaagtac cacaata
//
LOCUS       DAZKHC020000178         6158 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZKHC020000178 DAZKHC020000000
VERSION     DAZKHC020000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.178"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            173..805
                     /locus_tag="ACHOSM_RS22785"
                     /old_locus_tag="pgaptmp_004557"
     CDS             173..805
                     /locus_tag="ACHOSM_RS22785"
                     /old_locus_tag="pgaptmp_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HGU4560908.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQNQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1318..2568
                     /gene="frc"
                     /locus_tag="ACHOSM_RS22790"
                     /old_locus_tag="pgaptmp_004558"
     CDS             1318..2568
                     /gene="frc"
                     /locus_tag="ACHOSM_RS22790"
                     /old_locus_tag="pgaptmp_004558"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HGU4560909.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            2622..4316
                     /gene="oxc"
                     /locus_tag="ACHOSM_RS22795"
                     /old_locus_tag="pgaptmp_004559"
     CDS             2622..4316
                     /gene="oxc"
                     /locus_tag="ACHOSM_RS22795"
                     /old_locus_tag="pgaptmp_004559"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HGU4560910.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            4386..5330
                     /gene="yfdV"
                     /locus_tag="ACHOSM_RS22800"
                     /old_locus_tag="pgaptmp_004560"
     CDS             4386..5330
                     /gene="yfdV"
                     /locus_tag="ACHOSM_RS22800"
                     /old_locus_tag="pgaptmp_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HGU4560911.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            5404..>6158
                     /gene="yfdE"
                     /locus_tag="ACHOSM_RS22805"
                     /old_locus_tag="pgaptmp_004561"
     CDS             5404..>6158
                     /gene="yfdE"
                     /locus_tag="ACHOSM_RS22805"
                     /old_locus_tag="pgaptmp_004561"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HGU4560912.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITI"
BASE COUNT         1691 a         1303 c         1443 g         1721 t
ORIGIN      
        1 ctctgcgaca ggaagcagat tcctacaatt gtaagactaa aatacttctt gcgataataa
       61 ctacaactgt aagataaccc tttcaaaatg accgttgctc tctgatttct catttcatgc
      121 tcacccaata tgatggcggc gttttctaaa actgttaaag aatgaggtaa gtatgaaacg
      181 tttaattatg gctacgatgg tcacagcaat tctggcatct tcaactgtat gggctgctga
      241 taatgcaccg gtagcggcac aacagcaaac tcagcaagtt cagcaaaacc agaaaacggc
      301 agcagcagag cgtatctctg aacaaggttt atatgcgatg cgtgacgtgc aggtagcgcg
      361 cctggctctg tttcatggcg atccagaaaa ggcaaaagaa ctgaccaatg aagcttccgc
      421 tttgctgtca gatgacagta ccgaatgggc aaagttcgct aagccaggta aaaagaccaa
      481 tctcaatgat gaccaatata ttgtcattaa tgcatctgtc ggaatttctg agagttatgt
      541 agcaactccc gaaaaagaag ccgcgataaa aattgccaat gaaaaaatgg cgaaagggga
      601 taaaaagggc gcaatggaag aacttcgcct ggccggtgtt ggcgtaatgg aaaaccaata
      661 tctgatgccg cttaaacaga ctcgtaatgc acttgctgat gcgcagaaac tgcttgataa
      721 aaagcaatac tacgaggcaa acctggcgct taaaggtgcg gaagatggaa tcatcgtcga
      781 cagcgaagca ctgtttgtga actaatgctg acaggcagta tattgaggta aaggatttaa
      841 ctccctccag ccgacaattt tttatgacca caatacgaca ggttgtggtc ataaaattat
      901 ttggcgctcg cggttctata aacacgcatt ttatcttcct caaagtaaac cccaataatc
      961 ccgtgtcatc acaaatcatt tattatgtta tgttttatga tacgctttaa aaagcgtaat
     1021 cataccctta cttgtaaatc accggcgctt cttacagttg taagaataac atcacaaatg
     1081 tcgcgactct ctctgattac cacgaatcaa tatttatcat attcttcttt tcgtcctcac
     1141 tacttaccat gcagaaagga attggaagtg gcgatgaaat gattttcagc acttgataca
     1201 ggattttaaa aatatcgagt gcagctacga cgatattttg cgggttattt atagaagata
     1261 ttacgcctta aataaacgct aaattaacac atatttaatt aactaatagg tattgctatg
     1321 tcaactccac ttcaaggaat taaagttctc gatttcaccg gtgtgcaatc tggcccatct
     1381 tgtactcaaa tgctggcctg gtttggcgct gacgttatta aaattgaacg tcccggcgtt
     1441 ggtgacgtaa cgcgtcacca gctgcgagat attcctgata tcgatgcgct ttacttcacc
     1501 atgcttaaca gtaacaaacg ttctattgag ttaaatacca aaacagcgga aggcaaagag
     1561 gtaatggaaa agctgatccg cgaagctgat atcttagtcg agaactttca tccaggggcc
     1621 attgatcaca tgggcttcac ctgggagcat attcaagaaa tcaatccacg tctgattttt
     1681 ggttcgatca aagggtttga tgagtgttcg ccttatgtga atgtaaaagc ctatgaaaac
     1741 gttgctcagg cagcgggtgg cgcggcatcc actacgggtt tttgggatgg tccgccgctg
     1801 gtaagcgctg cagcgttggg tgacagcaac accggaatgc atttgctgat cggtttactt
     1861 gctgctttgc tgcatcgcga aaaaacgggg cgtgggcaac gagtcaccat gtcaatgcag
     1921 gatgccgtat tgaacctttg ccgcgtgaaa ttacgtgacc agcagcgtct cgataaattg
     1981 ggttatctgg aagaataccc gcagtatccg aatggtacat ttggtgatgc agttccccgc
     2041 ggtggtaatg caggtggtgg cggtcagcct ggctggatcc tgaaatgtaa aggctgggaa
     2101 accgatccta acgcctatat ttatttcact attcaggagc aaaactggga aaacacctgt
     2161 aaagccatcg gcaaaccaga atggattacc gatccggcat acagtacagc ccatgcccga
     2221 cagccacata ttttcgatat ttttgctgaa atcgaaaaat acactgtcac tattgataaa
     2281 catgaagcgg tggcctattt gactcagttt gatattcctt gtgcaccggt tttaagtatg
     2341 aaagaaattt cacttgatcc ctctttgcgc caaagtggca gtgttgttga agtggaacaa
     2401 ccgttgcgtg gaaaatatct gaccgttggt tgtccaatga aattctctgc ctttacgccg
     2461 gatattaaag ctgcgccgct attaggtgaa cataccgctg ctgtattgca ggagctaggt
     2521 tatagcgacg atgaaattgc tgcaatgaag caaaaccacg ccatctgata ttaacggggg
     2581 cttctggctc ccaatttata aaaatttcga ggttattaat catgtcagat caacttcaaa
     2641 tgacagatgg tatgcatatc atcgttgaag cattaaaaca gaataatatt gacactattt
     2701 atggtgttgt aggtattcct gtgacggata tggcacgcca tgcccaggcg gaaggcattc
     2761 gttatattgg ttttcgtcat gagcagtcgg caggctatgc cgctgcggca agcggttttc
     2821 ttacccaaaa accggggatc tgcctgacag tttctgcgcc aggattcctc aatggtttga
     2881 ccgcattggc caacgcaacg gtaaatggtt ttccgatgat catgattagc ggctccagcg
     2941 accgcgcgat cgtcgaccta cagcaaggtg attatgaaga gctggaccaa atgaatgcgg
     3001 caaaaccgta tgccaaagca gcatttcgcg ttaatcagcc gcaggatctt ggcattgcat
     3061 tggcacgcgc tatccgggtc tctgtatcgg gtcgccctgg cggagtttat cttgatttgc
     3121 cagcaaatgt cctggccgcg acgatggaaa aagacgaagc gttaaccacg attgttaaag
     3181 ttgaaaatcc gtcgccagca ttattgccat gcccgaagtc agtcactagc gcaatttcgc
     3241 ttttagcaaa agctgaacgg ccattaatta tccttggcaa aggcgcggcg tattcacaag
     3301 ctgatgaaca gcttcgtgaa tttattgaaa gtgctcagat tccattcctg ccaatgtcta
     3361 tggcgaaagg gatccttgaa gatacgcatc cactttctgc ggcagctgcg cgttcgtttg
     3421 ccctggcaaa tgctgacgtt gtcatgcttg ttggtgcacg actgaattgg ttattggcac
     3481 acggtaaaaa aggatgggcg gcagatacac agtttattca actggatatt gaaccgcagg
     3541 aaattgacag caaccgcccc attgctgtgc cagtcgttgg tgatattgca tccagtatgc
     3601 aaggtatgct ggcagaactg aaacaaaaca catttacgac tccactggta tggcgcgata
     3661 ttttaaatat ccacaagcag caaaatgcac aaaaaatgca tgaaaaatta agtacagata
     3721 ctcaaccatt aaattacttt aatgcattaa gtgctgtgcg cgacgtattg cgcgagaacc
     3781 aggatattta tttagttaat gaaggtgcaa ataccctgga taatgcacga aatattattg
     3841 atatgtataa accacgtcgt cgtctggatt gtggtacctg gggtgtcatg ggcatcggta
     3901 tgggctatgc catcggtgct agcgtgactt ctggttctcc ggttgtcgcc attgaaggtg
     3961 atagtgcttt tggtttcagt gggatggaaa ttgaaacgat ttgtcgatat aacctgccgg
     4021 tgacgatcgt tatttttaat aatggcggca tctacagagg agacggtgtt gatctcagtg
     4081 gcgctggtgc accatcacca acggatctgt tgcaccatgc aaggtatgac aaattaatgg
     4141 atgcgtttcg tggcgttggc tataacgtca ccacgacaga tgaacttcgt catgctttaa
     4201 ccaccggtat tcagtcgcgc aaaccgacca ttattaatgt ggtcatcgac cctgcagcag
     4261 gaactgaaag tggccatatt accaaactta acccaaaaca agtcgctggt aattaaatac
     4321 tgacatcgtc tcctcttgcc aactcattat gcgatggtga ggggaccctt atcggaaata
     4381 ctattatgct aacatttttt attggcgatt tattgcctat tatcgtaatc atgctgttgg
     4441 gttattttag cggcagacga gaaacatttt cagaagatca agctcgggca tttaataaac
     4501 tggtattaaa ctacgcgctt cctgcggctc tatttgtatc tattactcgg gcaaacaggg
     4561 aaatgatttt tgcggacact cgtctgaccc ttgtatcact tgtggttatt gtcggatgtt
     4621 tctttttctc ctggttcggt tgctacaaat tttttaaacg tacccatgca gaagcagctg
     4681 tatgtgcatt aattgcaggt tcacctacca ttggattcct ggggtttgca gttctcgatc
     4741 ctatttatgg tgattccgta tcaacaggtt tagtggtagc aattatttct attattgtta
     4801 acgcaattac tattcctatt ggtctgtatt tgctgaatcc ttcttcagga gcggatggta
     4861 agaagaatag taatctgagt gcattaattt ctgcggcaaa ggagccagta gtatgggcac
     4921 ctgttctggc aacgatcctg gtgttggttg gggtaaaaat tccggcagca tgggacccaa
     4981 cctttaatct gattgcgaag gctaactcag gggtagcggt attcgctgcg gggttgactc
     5041 tggctgcaca taaattcgag ttcagtgccg aaattgctta taacaccttc ctgaagctga
     5101 ttctgatgcc actggcactg cttctcgttg gtatggcatg tcatttgaac agcgaacatc
     5161 tgcagatgat ggtactggca ggcgcattac cgccggcatt ctccggaatc attattgcca
     5221 gccggtttaa tgtctacacc cgtactggta cagcgtcatt ggctgtgagc gtactgggct
     5281 ttgtcgtcac ggctcccttg tggatttatg tcagtcgact ggtttcataa ttaatcatgt
     5341 tattgatttt acataaatgc cgaagtgtct tttcaccttc ggcttttttc aaggcataag
     5401 caaatgacaa ataatgaaag caaagggccg tttgaaggct tattagttat cgatatgaca
     5461 catgtcctta atggaccttt cggaactcaa cttctttgta atatgggcgc aagggtaatt
     5521 aaagttgagc caccaggtca tggtgatgat acccgcacat ttggtcccta tgtggatgga
     5581 cagtcactct attacagttt tattaatcat ggcaaagaga gtgtggttct tgatttaaag
     5641 aatgatcacg ataaaagtat atttataaat atgctcaaac aagctgatgt attagctgag
     5701 aattttcgcc caggtacaat ggaaaaactg gggttttcat gggaaacgct tcaagaaatc
     5761 aacccgcgcc tcatatatgc ttcatcgtca ggtttcggac ataccggtcc gctaaaagat
     5821 gctcctgcct acgataccat cattcaggca atgagcggga taatgatgga aacaggatat
     5881 cctgatgctc cgccagtgcg cgttggtaca tctcttgcgg atctatgcgg cggtgtctat
     5941 ttattcagcg gaatagtgag tgcactttat ggccgcgaaa agagccagag aggggcgcat
     6001 gtcgatatag cgatgtttga tgccacgctg agttttctgg agcatggtct gatggcatat
     6061 atcgcaactg ggaagtcacc acaacgtctg ggaaatcgcc atccctacat ggcacctttt
     6121 gatgttttca atactcagga taagccgatt acgatttg
//
LOCUS       DAZKHC020000179         6145 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZKHC020000179 DAZKHC020000000
VERSION     DAZKHC020000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.179"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            85..861
                     /gene="exuR"
                     /locus_tag="ACHOSM_RS22810"
                     /old_locus_tag="pgaptmp_004562"
     CDS             85..861
                     /gene="exuR"
                     /locus_tag="ACHOSM_RS22810"
                     /old_locus_tag="pgaptmp_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HGU4560913.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1101..1184
                     /gene="yqiM"
                     /locus_tag="ACHOSM_RS22815"
                     /old_locus_tag="pgaptmp_004563"
     CDS             1101..1184
                     /gene="yqiM"
                     /locus_tag="ACHOSM_RS22815"
                     /old_locus_tag="pgaptmp_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HGU4560914.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1206..1868
                     /gene="yqjA"
                     /locus_tag="ACHOSM_RS22820"
                     /old_locus_tag="pgaptmp_004564"
     CDS             1206..1868
                     /gene="yqjA"
                     /locus_tag="ACHOSM_RS22820"
                     /old_locus_tag="pgaptmp_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HGU4560915.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1872..2255
                     /gene="mzrA"
                     /locus_tag="ACHOSM_RS22825"
                     /old_locus_tag="pgaptmp_004565"
     CDS             1872..2255
                     /gene="mzrA"
                     /locus_tag="ACHOSM_RS22825"
                     /old_locus_tag="pgaptmp_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HGU4560916.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2402..2770
                     /gene="yqjC"
                     /locus_tag="ACHOSM_RS22830"
                     /old_locus_tag="pgaptmp_004566"
     CDS             2402..2770
                     /gene="yqjC"
                     /locus_tag="ACHOSM_RS22830"
                     /old_locus_tag="pgaptmp_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HGU4560917.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2808..3113
                     /gene="yqjD"
                     /locus_tag="ACHOSM_RS22835"
                     /old_locus_tag="pgaptmp_004567"
     CDS             2808..3113
                     /gene="yqjD"
                     /locus_tag="ACHOSM_RS22835"
                     /old_locus_tag="pgaptmp_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HGU4560918.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3116..3520
                     /gene="yqjE"
                     /locus_tag="ACHOSM_RS22840"
                     /old_locus_tag="pgaptmp_004568"
     CDS             3116..3520
                     /gene="yqjE"
                     /locus_tag="ACHOSM_RS22840"
                     /old_locus_tag="pgaptmp_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU4560919.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3510..3809
                     /gene="yqjK"
                     /locus_tag="ACHOSM_RS22845"
                     /old_locus_tag="pgaptmp_004569"
     CDS             3510..3809
                     /gene="yqjK"
                     /locus_tag="ACHOSM_RS22845"
                     /old_locus_tag="pgaptmp_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HGU4560920.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWSLVKTTLKQQQLRG"
     gene            3995..4387
                     /gene="yqjF"
                     /locus_tag="ACHOSM_RS22850"
                     /old_locus_tag="pgaptmp_004570"
     CDS             3995..4387
                     /gene="yqjF"
                     /locus_tag="ACHOSM_RS22850"
                     /old_locus_tag="pgaptmp_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU4560921.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4457..5443
                     /gene="yqjG"
                     /locus_tag="ACHOSM_RS22855"
                     /old_locus_tag="pgaptmp_004571"
     CDS             4457..5443
                     /gene="yqjG"
                     /locus_tag="ACHOSM_RS22855"
                     /old_locus_tag="pgaptmp_004571"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HGU4560922.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5736..6101
                     /gene="yhaH"
                     /locus_tag="ACHOSM_RS22860"
                     /old_locus_tag="pgaptmp_004572"
     CDS             5736..6101
                     /gene="yhaH"
                     /locus_tag="ACHOSM_RS22860"
                     /old_locus_tag="pgaptmp_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4560923.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1545 a         1518 c         1542 g         1540 t
ORIGIN      
        1 caccaaattt atatatgacc aatggcatac ccatgaataa agtggtatga taacttcgtc
       61 cgcattccac tccgggagac gcatatggaa atcactgaac cacgccgttt gtatcaacaa
      121 cttgccgctg acctgaaaga gcgcatcgaa cagggcgtct atctggtggg tgataaactg
      181 cctgcagaac gctttattgc cgatgaaaag aacgtcagcc gtacggtagt tcgtgaagcc
      241 atcatcatgc tggaagttga aggctatgtc gaagtgcgta aaggttcagg tattcatgtg
      301 gtttccaacc agccgcgcca tcagcaggca gctgacaata atatggaatt cgccaattac
      361 ggtccgtttg agttgcttca ggctcgccag ctcatcgaaa gtaatattgc cgaattcgcg
      421 gcaactcagg taacgaaaca ggacatcatg aaactgatgg ccattcagga acaggcgcgc
      481 ggcgaacaat gcttccgtga ttccgagtgg gatttacagt tccacattca ggtagccctg
      541 gcgacgcaga actccgccct ggcggccatc gttgaaaaaa tgtggaccca acgtagccat
      601 aacccgtact ggaaaaaact acacgaacac attgatgccc gtactgtcga taactggtgc
      661 gatgaccacg atcaaatcct caaggcgctg attcgcaaag atcctcatgc tgctaagctg
      721 gcaatgtggc agcatctgga aaacaccaaa atcatgttat ttaacgaaac cagcgacgat
      781 ttcgagttca atgccgaccg ctatctgttc gcagaaaacc cggtagtgca tctcgatacg
      841 gccaccagcg gcagcaaatg aaatttccat ccgacaggcg ctgcaatgcg cctgtttgct
      901 tcatcggtaa gcgaaaagta taaagtgtca gcctgtgtaa atcctctcgc caccctcccc
      961 tgcaatcagc aaaatcagac tccacggaca tgtaattttg ataacgaaca acgttgacct
     1021 ttgttacaat tagattcaat ttgaatttat gcttttgaat gctttcttat ctcacgattt
     1081 aacagggaat agttcaggct gtgttgatgt atcaaacccg cagaacatac caaaacagca
     1141 ataacattgc ggtagtgcat cttttaaaac cagcgtggcg ttaaccgatt caccaggaat
     1201 aatgaatgga acttttgacc caattgctgc aagccctgtg ggcgcaggat tttgaaaccc
     1261 tggccaatcc atcgatgatt ggcatgttgt attttgtctt gtttgtaatt ttgttccttg
     1321 aaaacggctt gcttccagcg gcctttttac cgggcgacag tttactggta ttggtcggcg
     1381 tgttgattgc gaaaggcgcg atgggttacc cgcaaacgat tctgctgctg accgttgccg
     1441 ccagcctcgg ctgctgggtc agctatattc aggggcgatg gctgggcaat acccgcaccg
     1501 tacaaaactg gctatctcat ttacccgcgc attatcatca acgcgcacac catctttttc
     1561 ataaacacgg tttatcggcg ctgttaattg gtcgctttat tgcgtttgtc agaacactgc
     1621 tgccgacgat tgccgggtta tcagggctga ataacgcgcg ctttcagttt ttcaactgga
     1681 tgagcggtct gctgtgggta ttgatcctga caactctggg ttacatgctc ggcaaaacgc
     1741 cggtattttt aaagtacgag gaccagctga tgtcatgcct gatgctgctc ccggtggtgc
     1801 tgctggtgtt tggcctggca ggttctctgg tcgtgttatg gaaaaagaaa tatggaaatc
     1861 gggggtaagg gatgcaaata cctcgcatgt cgcttcgcca gctagcctgg tccggcactg
     1921 ttttacttct ggtcggcacg ctgttgctgg cctggtccgc ggttcgccag caagagtcta
     1981 cgctggcgat tcgtgccgtt catcaaggca caacgatgcc agacggtttt tcgatctggc
     2041 atcaccttga cgctcatggc attcctttca aaagtatcac ccccaaaaac gacactctgt
     2101 taattacatt tgactccagc gaccagagcg ccgccgcaaa agcggtcctc gacagaacat
     2161 tgccccatgg ctacatcatt gcgcagcagg acaataacag tcaggctatg cagtggctga
     2221 cccggttacg ggataactct catcgcttcg gataacttcc tgttatccga aacatatcac
     2281 tcactttggt gatttcacca taactgtcta tgattaatga ggcggtggtt aacactaccg
     2341 cttcgttgtc tggacaatcg ttcctttgta ataggtccga atcacaatgg aaggttcaag
     2401 aatgaaatac cgcatcgctt tagctgtttc tctctttgct cttagtgccg gtagttatgc
     2461 cactaccctg tgtcaggaaa aggagcaaaa tatccttaag gagatcagct atgccgaaaa
     2521 acaccaaaac cagaatcgta ttgacggtct gaataaagcc ctgagtgaag tccgggccaa
     2581 ctgttcagat agccagctgc gtgccgatca tcacaagaaa atcgcaaagc agaaagatga
     2641 ggtggcggaa cgccagcaag atttagccga ggcgaagcaa aaaggcgatg ccgataagat
     2701 tgccaaacgc gaacggaaac tggcagaagc gcaggaagag ctgaaaaagc tggaagcgcg
     2761 cgactactaa ctcacaatag tcactactta ctcacctgga gaaaactatg tcgaaagaac
     2821 acactacgga acatctgcgt gctgagttga aatccctttc cgatacgctg gaagaggtgc
     2881 ttagctcatc tggcgagaag tcgaaagaag agttgagtaa gattcgtagc aaagcggagc
     2941 aggcactgaa acagagccgt tatcgcctgg gtgaaaccgg tgatgccatc gccaaacaaa
     3001 cccgtgtcgc ggcggcgcgt gctgatgagt atgtgcgtga aaatccgtgg accggcgtgg
     3061 gcattggcgc tgcaatcggt gtagtgctcg gcgttctgct gtcgcgtcgt taattatggc
     3121 ggacactcat cacgcgcaag ggcccggtaa aagcgttctg ggcatcgggc agcgaattgt
     3181 ttccatcatg gttgaaatgg tggaaacacg tctgcggctg gcggtggtgg agctggaaga
     3241 ggaaaaagct aatctctttc aacttttact gatgctgggc ctgacgatgc ttttcgctgc
     3301 atttggtctt atgagcctga tggtgctaat tatttgggcg gttgatccgc aatatcgcct
     3361 gaatgcgatg attgccacca ccgtggtgtt gctgctactg gcactgattg gcggtatctg
     3421 gacgttacgt aaatcgcgta aatccacctt gctgcgccat acacgccatg agctggcaaa
     3481 cgatcggcaa ctgctcgagg aggagtcccg tgagcagtaa agtcgaacgt gaacgacgta
     3541 aggcgcaact gcttagccag atccagcaac aacggctgga tctttccgcc agtcgtcgtg
     3601 aatggctgga ggcaacaggc gcttacgatc gtcgctggaa tatgctgcta agtctgcgct
     3661 cctgggcgct ggttggcagt agcgtgatgg cgatctggac gattcgccat cctaatatgc
     3721 tggtccgctg ggccagacgc ggttttggcg tatggagcgc ctggagtctg gttaaaacga
     3781 ccctcaagca gcaacagctt cgcggttaac ttccctctgg ccggagcaat tccggcctta
     3841 tccctcaaat tttttgaaga tttttgacag ttttccttgc taacaatcat cattcaccac
     3901 gtttatgatt ctctccatcg acagcaacga cgctaatacc gcgcccttgc acaaaaaaac
     3961 aatcagcagc ctgagtggcg cagtggagag tatgatgaaa aaattagaag atgttggtgt
     4021 actggtagcg cgcattttaa tgccgattct gtttattacc gcaggctggg gaaaaatcac
     4081 cgcttactct ggtacccaac aatatatgga agcaatgggt gtcccgggtt ttatgctgcc
     4141 gctggtgatt ctgcttgagt tcggtggtgg tctggcaatc ctgttcggtt tcctgactcg
     4201 caccacagcc ctgtttactg cgggctttac gctgctgacg gcatttttat ttcacagcaa
     4261 ctttgctgaa ggcgtcaact cgctgatgtt catgaaaaac ctgacaattt ctggcggatt
     4321 cctgctgctg gcaattaccg gtccgggcgc gtatagcatc gaccgcctgc tgaataaaaa
     4381 gtggtaagca cgcgctatac ttaacgataa aaaacgagga ggaagctcct cgtttttgct
     4441 attggaggag agaaaaatgg ggcaactgat tgacggcgtc tggcatgaca cctggtacga
     4501 taccaaatct accggcggta aatttcaacg ttcagcttcc gcatttcgta actggctcac
     4561 tgccgatggc gcacctgggc ccactggcac aggcggtttt atcgcagaga aagatcgtta
     4621 tcatctctat gtttcactcg cctgcccgtg ggcgcaccgc acgctgatca tgcgcaaact
     4681 caaaggactg gaaccgttta tttccgtttc cgtagtgaac ccgctgatgc tggaaaacgg
     4741 ctggaccttt gatgacagtt ttccgggagc aaccggagat acgctctatc aacatgaatt
     4801 tctgtatcag ctttatctcc acgccgatcc acactacagc ggacgagtta ctgttcccgt
     4861 gctgtgggac aaaaagaacc acaccatcgt cagcaacgaa tcagcagaaa tcatccgcat
     4921 gttcaatacc gcgtttgatg cgctgggcgc aaaagcgggt gattactacc caccagccct
     4981 gcaaacgaaa attgacgaac ttaacggctg gatttatgac accgttaaca acggcgtgta
     5041 taaagctggt tttgccacca gccagcaagc ctacgacgag gcggtggcga aagtgtttga
     5101 atcgctggcg cgactggagc agattttagg ccagcatcgt tacctgaccg gcaaccagct
     5161 aaccgaagcc gatattcgcc tgtggaccac gctggtccgt tttgatccag tgtatgtgac
     5221 ccacttcaag tgtgataagc accgcatcag cgattacctt aatctgtatg gcttcctgcg
     5281 tgatatctac cagatgccgg gaattgccga aacagtcaat ttcgatcaca tccgtaatca
     5341 ttacttccgc agccataaga ccatcaaccc tacggggatt atttcaattg gtccatggca
     5401 ggatctcgat gaaccgcatg gacgagatgt tcgcttcggt taaaaaaagg caccttacga
     5461 ggtgcctctg ataattagct aagtgcatgt tgttcatgtc ggatgcggcg tgaacgcctt
     5521 atcaaaccta caaaacattt atattcaata tgttgcaatg accgcgcagg ccagataagc
     5581 gtagcgcatc tggcgatttt gcgcttgtca tcagtcttag cgcgccttca cagcacttta
     5641 ttaattcctt gaacgaatat ttactgccat ttcattcacg tctattctta atttgctgct
     5701 ttaaaaacaa gtgattgagc aaaattgagg caaaaatgga ctggtatctg aaagtactaa
     5761 aaaattatgt cggtttccgg ggtcgtgcgc ggcgcaaaga gtactggatg tttattctgg
     5821 tcaacatcat ctttacgttc gtgctggggc tgctggataa aatgttaggc tggcaacgcg
     5881 ctggtggcga aggcatcctg acgacaatct acggtattct ggtgttttta ccgtggtggg
     5941 cagttcagtt ccgccgcctg cacgacaccg accgctcggc gtggtgggca ctgctattct
     6001 taatcccgtt tatcggctgg cttattatca tcgtctttaa ctgccaggcg ggtacgccag
     6061 gcgaaaaccg ctttggacct gatccaaaac tggaacctta aaacaaatct gctcgccgga
     6121 tgtctgactc atccggcttt tatca
//
LOCUS       DAZKHC020000180         6103 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZKHC020000180 DAZKHC020000000
VERSION     DAZKHC020000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.180"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..396)
                     /locus_tag="ACHOSM_RS22865"
                     /old_locus_tag="pgaptmp_004573"
                     /pseudo
     CDS             complement(1..396)
                     /locus_tag="ACHOSM_RS22865"
                     /old_locus_tag="pgaptmp_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            537..2969
                     /gene="hsdR"
                     /locus_tag="ACHOSM_RS22870"
                     /old_locus_tag="pgaptmp_004574"
     CDS             537..2969
                     /gene="hsdR"
                     /locus_tag="ACHOSM_RS22870"
                     /old_locus_tag="pgaptmp_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HGU4560924.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDEEQVTGVDEPPAPPYQVTDSDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            3036..4505
                     /locus_tag="ACHOSM_RS22875"
                     /old_locus_tag="pgaptmp_004575"
     CDS             3036..4505
                     /locus_tag="ACHOSM_RS22875"
                     /old_locus_tag="pgaptmp_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGU4560925.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4505..>6103
                     /locus_tag="ACHOSM_RS22880"
                     /old_locus_tag="pgaptmp_004576"
     CDS             4505..>6103
                     /locus_tag="ACHOSM_RS22880"
                     /old_locus_tag="pgaptmp_004576"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU4560926.1"
                     /translation="MSVEKLIVDHMETWTSVLQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVRLGNIAEYIQRGKGPKYSENGTVRVVSQKCVQWSGFKLEQSRWITDESIPSY
                     TEERFLKNGDVLWNSTGAGGTAGRVNFLETVKERLVVDSHVTIIRSIKQNGQFICNYI
                     STYGIQQLFDPKHPKTLLSGTTNQAELNTSVVISFITPFPPQKEQERINTKVIELMSL
                     CDQLEQQSLTSLYAHQQLVKTLLGTLTDSQNAEELAENWTRISEHFDTLFTTEASVDA
                     LKQTILQLAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKSLPPISDEEK
                     PFELPEGWEWCRLEEIAYIFSGNAFKSEDFNKSAGTKCIKITNVGVHEFIESHDYLPS
                     DFNKSYHNFRVYSGDMIIAMTRPYISSGLKICICPDSYHNALLNQRVCAIRLSHVSKY
                     YYLFLKSLFVLKHYQDRFNNSGLQP"
BASE COUNT         1872 a         1423 c         1399 g         1409 t
ORIGIN      
        1 ctctaactct aatataataa aaatacctcc cagaaaaaca aataatgaaa aagcactaaa
       61 aatataaaca gaggcactga ctggaaaaat attaattgaa agcacaaaga acaaaaatat
      121 aaatcatgca ataagtgaac accaagcagc acaactattc aacctgtttt tattactgaa
      181 cttaacaaaa taccccatat attctgtaat gcgattttct tccactaatg cataaagaag
      241 aataccaaca aagtacagag ataaaataat atctttccac agatatttat aagggagaag
      301 tataaaacca aggagtagat aaattagcag aatgacatct aatgccttag tacaaaaata
      361 ttttttcact ccttgcaaga accgaacatt ccccatatga atccttatcc agctattgat
      421 gaaactcaag aacaccttta aaacagtgac ttaacgctga taatactctt tgaacagatg
      481 aattcatcta tttttctgtg tagaatttgc gcatcgcatt tagatgagaa ccccccatgg
      541 cggaactgaa cctaagtaac ctgacggaag cagacatcat taccaaatgc gttatgccag
      601 ccattctcaa tgcaggctgg gacaacacaa cgcaaatcag acaggaggtc aaactccggg
      661 acggtaaagt catcgtacgt ggtaaagttg cggcacgcag aacggtaaaa tctgctgaca
      721 tcgtgctgta tcacaaacca ggcattccct tagccgtgat tgaagcaaaa gccaacaaac
      781 atgaaattgg caaagggatg caacagggca ttgaatatgc gcgcctgctg gacgttcctt
      841 ttgtttttgc caccaacggg gatggcttta tcttccgcga tgccaccgca gccgaaggtg
      901 aatgccttga aaagcaaata acgctggatg acttcccctc ccccgctgaa ctctggcaaa
      961 aattctgcct ctggaaagga tacacacaag ctcagcttcc ggtgattact caggactatt
     1021 acgacgatgg cagcggtaaa tcgccacgtt attaccagct tcaggcaatc aacaaaacca
     1081 ttgaagccgt ctccaacggg caaaaccgcg ttctgctggt catggcgacc ggaacaggga
     1141 aaacctatac cgcattccag atcatctggc gcctgtggaa atcaaaaaat aaaaaacgca
     1201 ttttgttcct tgccgatcgc aacattctgg tcgaccaaac caaaaataat gatttccagc
     1261 catttggtac ggcaatgacc aaagtcagcg gacgcaccat tgatcccgct tatgaaattc
     1321 acctcgcgct ctatcaggct ataactggcc cggaggaaga ccaaaaagcg tttaaacaag
     1381 tcgcaccaga tttcttcgat ctgatcgtga tcgacgaatg ccatcgcggc agcgcatccg
     1441 aagacagcgc ctggcgagag atccttgatt atttcagttc cgccacccaa attggcttaa
     1501 ccgccacgcc aaaagagacg catgaagtct ccagcacgga ttacttcggc gatccggttt
     1561 acgtctactc gctaaaagaa gggatcgaag acggcttcct cgccccttat aaagttgtcc
     1621 gtgttgatat tgatgttgat ctgcaaggct ggcgcccaac caaagggcaa actgacttaa
     1681 acggcgaagt gatcgacgat cgtatctata accagaaaga tttcgatcgc acgatggtaa
     1741 tcgacgaacg cacagaactg gttgccagaa ccattaccga ctacctcaag cgcaccaacc
     1801 cgatggataa aaccatcgtc ttctgtaacg acatcgatca tgcagaacgt atgcgccgcg
     1861 ccctggttaa tctcaacccg gagcaggtga aaaagaacga caaatacgtc atgaaaatca
     1921 ccggcgatga tgaaattggc aaagctcagt tggataactt catcaacccg aaaaaaccgt
     1981 acccggttat cgcgaccact tcagagctga tgaccactgg tgtggatgct aaaacctgca
     2041 aactggtagt actggaccag aacatccagt cgatgaccaa attcaagcaa attatcggtc
     2101 gtggtacacg catcgacgaa cgttacggca aactctggtt taccatcctc gactttaaaa
     2161 aagccaccga actgtttgcc gatgagcgtt tcgatggcat tcccgaaaaa gtcatggata
     2221 ccacaccaga ggatatcgcc gatccagaat ctgattttga agagaaactc gaagaaatca
     2281 gcgaacatga cgaagaacag gtaacaggcg ttgatgaacc gcctgcgcca ccataccagg
     2341 ttaccgatag cgatgatgtc ggcccacttc cggaagaaga cgagaagaaa atccgcaagt
     2401 ttcacgtcaa cggtgtggcg gtgggcgtta ttgcacagcg tgttcagtat tacgatgccg
     2461 acggaaaact ggttaccgaa tcctttaaag attacacccg caaaacactg ctcaaagaat
     2521 atgcctcgct ggatgacttt acccgcaagt ggcaggacgc cgatcgcaaa gaagcgatca
     2581 ttcacgagct ggaacaacaa gggatcatct gggaagtgct ggcagaagaa gtcggtaaag
     2641 atctcgaccc gttcgacatg ctttgccacg tagtgtatgg tcagccgccg ttaacccgca
     2701 aagagcgcgc cgagaacgtg cgcaagcgga actacttcac aaaatactct gaagcagcgc
     2761 aagccgtgct cgataatctg ctggataaat acgccgatgc gggcgtccag gagatcgaaa
     2821 gtattcaggt gctgaaactt aagccattcg acagcatggg caccttacca gagattatta
     2881 aaaccggatt tggcgaccgt aacgggtata atcaggcgct cagcgagctg gaaaacgaaa
     2941 tctaccaatt accgccccgc tctgcttaac aactctcttt tgcgccttct gcaaccatca
     3001 tgttgcagaa ggccttgttt ttcatggaat acaacatgtc tatcagttca gtaatcaaat
     3061 cattacaaga tattatgcgc aaagatgccg gtgtggacgg cgatgcgcag cgtctcggtc
     3121 agctctcctg gctgctgttt ttgaaaatct tcgatgccca ggaagaggcg ctggaactgg
     3181 agcaggataa ctatcaatat ccgatcccac agcgttattt gtggcgcagt tgggccgcaa
     3241 acgctcaggg cattaccggt gattccctgc tggaattcgt taatgatgat ctgttcccgg
     3301 cgttgaaaaa cctcactgcg cctatcgata aaaacccacg cggctacgtg gtgaagcagg
     3361 cgttcagcga tgcctataac tatatgaaaa acggtacgct actgcgtcag gtgatcaaca
     3421 agctgaacga aattgacttt accagcgcca gcgaacgcca tctgtttggt gatatttacg
     3481 aacaaatcct taaagatctg caatctgcgg gcaatgctgg cgaattctat actccacgcg
     3541 ccgtcactcg ctttatggtg gatcgcgttg atccgaaact cggcgaatcc attatggacc
     3601 cggcctgcgg tacgggcggt tttcttgcct gcgcatttga tcatgtaaag aacaaatacg
     3661 tgaagagcgt cgccgatcat cagacgctgc aacaacagat ccacggtgtt gagaaaaaac
     3721 agcttccgca cctgctggcg accaccaata tgctgctgca cggcattgaa gtaccggtac
     3781 aaattcgtca cgacaacacc ctgaacaaac cgctttcctc ctgggatgaa caactggatg
     3841 tcattgttac caacccgccg tttggtggca cggaagaaga cggtattgag aagaactttc
     3901 cggcagagat gcaaacccgc gaaacggcgg atctgttcct gcaactgatt gtggaagtac
     3961 tggcgaaaaa cggtcgcgcg gcggtggtat tgccggatgg cacactattt ggcgaaggcg
     4021 ttaaaaccaa aatcaaaaaa ctgcttaccg aagagtgcaa cctgcatacc atcgtgcgtt
     4081 taccgaatgg ggtgtttaac ccctataccg gcattaaaac caacctgttg ttctttacca
     4141 aaggtcagcc aaccaaagag atttggttct atgagcatcc gtatccggcg ggcgtaaaaa
     4201 actacagcaa aaccaagccg atgaagtttg aagagtttca ggcggagatt gactggtggg
     4261 gtaacgaggc cgatggtttt gccagccgcg tcgagaatga gcaggcgtgg aaagtcagca
     4321 ttgatgacgt gattgcgcgt aacttcaatc tggatattaa aaacccacat caggcggaaa
     4381 ccgtcagcca tgatccagat gaattgttag cacagtatgc aaagcagcag gcggagatcc
     4441 agacgctgcg taatcaactg cgcgatattc ttggcgctgc gctgtctggc aaggaggtta
     4501 actaatgagt gtggaaaagc tgatcgttga tcatatggaa acctggacct cggtgttgca
     4561 aacccgctcc accgccgggc gcggcagttc cggtaaaatt gatttgtatg gcattaagaa
     4621 attgcgtgag ctgattctgg aactggctgt gcgcggtaaa ctggtgccgc aggatccgaa
     4681 tgacgaacca gcgtcggagc tgttgaagcg tattgcggcg gaaaaagcag agctggtgaa
     4741 gcaaggtaaa attaaaaagc aaaaaccgct gccggaaatt agcgaggaag agaagccgtt
     4801 tgaattgccg gagggatggg agtgggtgcg attgggcaat atcgctgaat acatccagcg
     4861 aggtaaaggt ccaaaatact cagaaaatgg aacagtaaga gtcgtatctc aaaaatgtgt
     4921 gcagtggagt ggctttaaat tagagcagtc cagatggatt actgatgaat ctattccctc
     4981 atatacagaa gagcgttttt taaaaaatgg tgatgttctc tggaactcaa caggggctgg
     5041 tggaactgca ggtcgagtta attttctcga aactgttaag gaaaggcttg tagttgatag
     5101 tcatgtaacg ataattagaa gcattaaaca aaatggtcaa tttatctgca attatatttc
     5161 aacttatgga attcagcagc tttttgaccc gaaacatcct aaaactttat tatctggcac
     5221 aactaaccaa gctgaactca acacttctgt tgtgatatct tttataactc cttttcctcc
     5281 tcaaaaagag caagaaagga taaatactaa agtcatagag ttaatgtctc tctgcgacca
     5341 actggaacag caatccctaa ccagtctgta cgcacatcag caactggtta aaaccctgtt
     5401 gggaacgctt acagacagcc aaaacgccga ggaactggct gaaaactgga cgcgtattag
     5461 cgagcatttc gacacactat ttaccaccga agccagcgtg gatgcgttaa aacagaccat
     5521 tctgcaactg gccgtaatgg gtaaacttgt gccgcaggat ccgaatgacg aaccagcctc
     5581 cgaactgctc aaacgtattg cgcaggaaaa agctcaactg gtgaaagaag gaaaaataaa
     5641 aaaacaaaaa tcgttgccac cgattagcga tgaggaaaaa ccgtttgaac ttccggaagg
     5701 gtgggagtgg tgtcgcttag aggagatagc ttatattttc tccggcaatg cctttaaaag
     5761 tgaagatttc aataaatcgg ctggaacgaa atgtataaaa ataaccaatg tgggtgtaca
     5821 cgaattcata gagtcacacg attacctgcc ttctgatttt aacaagtcat accataattt
     5881 ccgagtttat tctggtgaca tgattattgc catgacaaga ccatacatta gttccggctt
     5941 gaaaatttgt atttgtcctg atagctacca caatgctctt cttaaccaac gagtctgtgc
     6001 gatccgttta tctcatgttt caaagtatta ttatctattt ttaaagagcc tattcgtatt
     6061 aaaacattac caagatagat ttaataattc aggtttgcag ccg
//
LOCUS       DAZKHC020000181         6080 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZKHC020000181 DAZKHC020000000
VERSION     DAZKHC020000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.181"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            62..367
                     /gene="ulaB"
                     /locus_tag="ACHOSM_RS22885"
                     /old_locus_tag="pgaptmp_004577"
     CDS             62..367
                     /gene="ulaB"
                     /locus_tag="ACHOSM_RS22885"
                     /old_locus_tag="pgaptmp_004577"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HGU4560927.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            377..841
                     /gene="ulaC"
                     /locus_tag="ACHOSM_RS22890"
                     /old_locus_tag="pgaptmp_004578"
     CDS             377..841
                     /gene="ulaC"
                     /locus_tag="ACHOSM_RS22890"
                     /old_locus_tag="pgaptmp_004578"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HGU4560928.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            855..1505
                     /gene="ulaD"
                     /locus_tag="ACHOSM_RS22895"
                     /old_locus_tag="pgaptmp_004579"
     CDS             855..1505
                     /gene="ulaD"
                     /locus_tag="ACHOSM_RS22895"
                     /old_locus_tag="pgaptmp_004579"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGU4560929.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            1515..2369
                     /gene="ulaE"
                     /locus_tag="ACHOSM_RS22900"
                     /old_locus_tag="pgaptmp_004580"
     CDS             1515..2369
                     /gene="ulaE"
                     /locus_tag="ACHOSM_RS22900"
                     /old_locus_tag="pgaptmp_004580"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HGU4560930.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            2369..3055
                     /gene="ulaF"
                     /locus_tag="ACHOSM_RS22905"
                     /old_locus_tag="pgaptmp_004581"
     CDS             2369..3055
                     /gene="ulaF"
                     /locus_tag="ACHOSM_RS22905"
                     /old_locus_tag="pgaptmp_004581"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HGU4560931.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLDNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(3185..3460)
                     /gene="yjfY"
                     /locus_tag="ACHOSM_RS22910"
                     /old_locus_tag="pgaptmp_004582"
     CDS             complement(3185..3460)
                     /gene="yjfY"
                     /locus_tag="ACHOSM_RS22910"
                     /old_locus_tag="pgaptmp_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HGU4560932.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEAHQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            3787..4182
                     /gene="rpsF"
                     /locus_tag="ACHOSM_RS22915"
                     /old_locus_tag="pgaptmp_004583"
     CDS             3787..4182
                     /gene="rpsF"
                     /locus_tag="ACHOSM_RS22915"
                     /old_locus_tag="pgaptmp_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGU4560933.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            4189..4503
                     /gene="priB"
                     /locus_tag="ACHOSM_RS22920"
                     /old_locus_tag="pgaptmp_004584"
     CDS             4189..4503
                     /gene="priB"
                     /locus_tag="ACHOSM_RS22920"
                     /old_locus_tag="pgaptmp_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HGU4560934.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            4508..4735
                     /gene="rpsR"
                     /locus_tag="ACHOSM_RS22925"
                     /old_locus_tag="pgaptmp_004585"
     CDS             4508..4735
                     /gene="rpsR"
                     /locus_tag="ACHOSM_RS22925"
                     /old_locus_tag="pgaptmp_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGU4560935.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            4777..5226
                     /gene="rplI"
                     /locus_tag="ACHOSM_RS22930"
                     /old_locus_tag="pgaptmp_004586"
     CDS             4777..5226
                     /gene="rplI"
                     /locus_tag="ACHOSM_RS22930"
                     /old_locus_tag="pgaptmp_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGU4560936.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(5297..>6080)
                     /locus_tag="ACHOSM_RS22935"
                     /old_locus_tag="pgaptmp_004587"
     CDS             complement(5297..>6080)
                     /locus_tag="ACHOSM_RS22935"
                     /old_locus_tag="pgaptmp_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGU4560937.1"
                     /translation="TTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDHNFDG
                     RRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKYNKAY
                     AGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPN
                     CWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIY
                     SGLGFERVPQDRDIRSDRMHLTLANHPDLWTQENGEWIYLRN"
BASE COUNT         1517 a         1455 c         1670 g         1438 t
ORIGIN      
        1 cgcagaagaa gatgcagaaa aacaactggc agaacagtct gcttaataag gagttttgat
       61 tatgaccgta cgtattctgg ctgtgtgtgg caacggacaa ggcagttcca tgatcatgaa
      121 gatgaaagtg gaccagtttt taacccaatc aaacattgac cacacggtaa atagctgcgc
      181 ggttggcgaa tacaaaagcg agttgagcgg cgcggatatc atcatcgctt ctacgcacat
      241 tgcgggcgaa atcaccgtga ctggcaacaa atacgtggtt ggcgtgcgca acatgctctc
      301 tcctgccgat tttggcccga aactgctgga agtgatcaaa gcgcatttcc cgcaggatgt
      361 gaagtaagga cgcgccatga aattacgtga ttcgctggcg gagaataaat ccatccgcct
      421 gcaggctgaa gctgagacat ggcaggacgc agtaaaaatc ggcgttgacc tgctggtggc
      481 ggcggatgtt gtcgagccgc gttactacca ggcgattctg gatgcggttg aacagcacgg
      541 tccgtacttt gtgctcgcac caggcctggc aatgccgcac ggtcgcccgg aagagggcgt
      601 taagaaaacc ggttttgcgc tggtgacgct gaaaaagccg ctggagttca accacgaaga
      661 taacgacccg gtggatatcc tcatcaccat ggcggcggtc gatgccaaca cgcaccagga
      721 agtgggcatc atgcagatcg tcaacctgtt tgaagatgaa gaaaatttcg accgtttacg
      781 tgcctgccgt accgagcagg aagtactgga tctcattgac cgcaccaacg cggcagctta
      841 agaaggaatt gaagatgtca ttaccgatgt tgcaagtcgc gctggacaac cagactatgg
      901 atagcgccta cgaaaccact cgcctgattg ccgaagaagt cgacattatc gaagtgggta
      961 ccattctgtg cgtgggcgaa ggcgtgcgtg cggttcgcga tctgaaagcg ctctacccgc
     1021 acaaaatcgt gctggcagac gccaaaattg ccgatgcggg caaaattctt tctcgtatgt
     1081 gcttcgaagc caacgctgac tgggtgaccg tgatttgttg tgcggatatc aacaccgcca
     1141 aaggtgcgct ggacgtagca aaagagttta acggcgacgt gcagatcgaa ctgaccggtt
     1201 actggacctg ggaacaggcg caacagtggc gcgacgcagg tattcagcag gtggtttatc
     1261 accgcagccg tgacgcgcag gccgctggcg tggcgtgggg tgaagccgat atcactgcga
     1321 tcaaacgtct ttccgatatg ggcttcaaag tcaccgtcac cggtggcctg gcgctggaag
     1381 atctgccgct gttcaaaggc attccaattc atgtgtttat cgccggtcgc agcatccgtg
     1441 atgccgcttc tccggttgaa gccgcgcgtc agttcaaacg ttccatcgcc gaactgtggg
     1501 gctaaggagc ggatatgttg tccaaacaaa tcccgcttgg catctatgaa aaagcgctcc
     1561 ccgccgggga gtgctggctg gaacgcctgc gactggcaaa aacgttgggc ttcgattttg
     1621 tcgagatgtc ggtagatgaa actgacgagc gcctgtcgcg cctcgactgg agccgcgagc
     1681 agcgtctggc gctggtgaat gcgattgttg aaaccggcgt gcgtgtgccg tccatgtgcc
     1741 tttctgccca tcgtcgtttc ccgttgggca gcgaagatga cgcggtgcgg gcgcaggggc
     1801 tagagattat gcgtaaagcc atccagttcg cccaggacgt tggcattcgc gtgatccagc
     1861 tggcgggcta tgacgtttac tatcaggaag ccaataacga aacgcgtcgt cgtttccgtg
     1921 acggcctgaa agagagcgtt gagatggcga gccgcgcgca ggtgacgctg gcgatggaga
     1981 tcatggatta tccgttgatg aactccatca gcaaggcgct gggttacgcg cactatctca
     2041 acaacccgtg gttccagctc tacccggata tcggcaacct gtcggcgtgg gacaacgatg
     2101 tgcagatgga gttgcaggcc ggaatcgggc atatcgtcgc ggtgcatgtg aaagacacca
     2161 aaccgggcgt cttcaaaaac gtgccatttg gcgaaggtgt ggtggatttc gaacgttgct
     2221 tcgaaacgct caaacagagt ggctattgcg ggccgtacct gattgagatg tggagcgaaa
     2281 cggcggaaga cccggcggca gaagtggtga aagcgcgtga ttgggtgaaa gcgcgcatgg
     2341 ccaaagcagg catggtggag gcggcataat gcaaaagcta aaacagcagg tgtttgaagc
     2401 caacatggat ctgccgcgct atgggctggt gacctttacc tggggcaacg tcagcgctat
     2461 cgaccgcgaa cgcgggctgg tggtgatcaa gcccagcggc gttgcctacg aaaccatgaa
     2521 agcagacgat atggtggtgg tcgatatgag cggcaaggtg gtggaagggg agtatcgccc
     2581 atcttccgac actgcgacgc atctcgaact ctaccgtcgt tacccgtcgc ttggcggcat
     2641 tgtccatacc cactccactc atgccaccgc atgggcgcag gcggggctgg cgatcccggc
     2701 gttaggcacc acgcacgctg actacttctt tggtgatatc ccgtgtacac gtggattaag
     2761 cgaagaagag gtgcagggtg agtatgaact gaacactggc aaagtgatta tcgaaacgct
     2821 ggacaacgcc gagccgctgc atacgccggg aattgtggtg tatcagcacg gaccgttcgc
     2881 ctgggggaaa gatgcccacg acgcggtgca taacgcggta gtgatggaag aagtggcgaa
     2941 aatggcgtgg attgcgcgca gtattaatcc acaactcaat catatcgaca gctacctgat
     3001 gaataaacac ttcatgcgta agcacggtcc taacgcttat tacgggcaga agtaaaacac
     3061 gcgctgcgga aatttccttc ctcgggagat aactggtcta attccgcagc cgtttttcaa
     3121 aaaaaagccc cctgcgaagg gggcaaagca aactatggca atgtttcgtt ggttatacct
     3181 ggtgctagcg ataaatatcc gcgctggcgt gcatattgcc gttactcccc ggttcccgca
     3241 tcagaattac gtggtagtac gttgcgcctt gcgcatctgt ttcttcattt aatgcctgat
     3301 gtgcttcgct ttcagtggcg aaattatgat tgatataaat cacgcctaag ctctgtacgt
     3361 cgtccatatt tctggcctgg tggttattaa tttcaatggc tgcccatgta tttgcactta
     3421 gcaaaagcac agccagaagg gctaaaacac gactgaacat agatacctcc tcgacggctg
     3481 actttgtgtg ctctcctctg tgatgatctt ctgatttaat tttaatcaat gataaagaag
     3541 ttgatggtga ccatttctga tgcagttgtt cgaaaaaaca ctgtgacgga atgtgatgaa
     3601 cttcaaatca gggagttagt tcttttgagc gaaagatgag atttatttgt cggctggcct
     3661 tgagtttagc ggggcataca agtactataa cgcgtcattt ttcagccgac ctttaacacg
     3721 ttccttgcct ccccgggatt cggctgaccc agacaggagg ctgaataatc cgtaaggagc
     3781 aattcgatgc gtcattacga aatcgttttt atggtccatc ctgaccagag cgaacaggtt
     3841 ccgggcatga tcgagcgcta cactgctgcc atcactggtg cagaaggcaa gatccaccgt
     3901 ctggaagact ggggccgccg tcagctggct tacccgatca acaaactgca caaagcacac
     3961 tacgttctga tgaacgttga agctccgcag gaagtgatcg atgagctgga aactaccttc
     4021 cgcttcaacg atgccgttat ccgcagcatg gttatgcgta ccaagcacgc tgttaccgaa
     4081 gcatctccga tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga tttcgcaaac
     4141 gaaaccgctg atgatgctga agctggggat tctgaagagt aatttctgat gaccaaccgt
     4201 ctggtgttgt ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc atcaggaatt
     4261 cctcactgcc agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg ttttcaccgg
     4321 caggcgtggt gccaaatgcc cgttattgtt agcggacacg aaaaccaggc cattactcac
     4381 agtataacgg tcggcagtcg cataaccgtt caggggttca tttcatgcca caaggcaaag
     4441 aacggactga gcaaaatggt tttgcatgcc gagcagattg aattgataga ttctggagac
     4501 tagccatatg gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg cggaaggcgt
     4561 tcaagagatc gactataaag atatcgctac gctgaaaaac tacatcaccg aaagcggtaa
     4621 gattgtccca agccgtatca ccggtacccg tgcaaaatac cagcgtcagc tggctcgcgc
     4681 tatcaaacgc gctcgctacc tgtccctgct gccgtacact gatcgccatc agtaatcggt
     4741 cacggtccat taatacgact ttgagaggat aaggtaatgc aagttattct gcttgataaa
     4801 gtagcaaacc tgggtagcct gggtgatcag gtaaacgtta aagcgggcta tgctcgtaac
     4861 ttcctggtac cgcagggtaa agctgttcca gctaccaaga aaaacattga attcttcgaa
     4921 gcacgtcgcg ctgaactgga agctaaactg gctgaagttc tggcagctgc taatgctcgc
     4981 gctgagaaaa tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg cgacgaaggt
     5041 aaactgttcg gttccatcgg tactcgcgac atcgctgacg ctgtaactgc agctggcgtt
     5101 gaagtggcta agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac tggcgagcac
     5161 gaagtgagct tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa cgtagtagct
     5221 gaataattcg ttattcaacg agacgtaaaa acgccgacca gtggtcggcg ttttgctttc
     5281 tatttttcgt caggtattag tttcgcaagt agatccattc accattctct tgtgtccata
     5341 aatcaggatg gtttgctagc gttagatgca tacgatcact ccttatgtct cggtcctgcg
     5401 gcactcgctc gaaacctaaa cccgagtaga ttgctgctga atttgataag gaacgtagac
     5461 caattttcaa atcatggggt aaatcagaat acgcgtttag caatgcagca atgatgaatc
     5521 cgagggcatt agaaacgcct gtaacagtgc gaagttggtc gatataaaga tcgattgttg
     5581 gtagttcaga tgaccagcaa ttaggcattt gggcaggaag ggttacaacg tatcctatcg
     5641 tttggccggt atagtctgga gtaccataac caggtcccaa aaccacttcg tgtattgtgt
     5701 atttactgtt gggttcgaag gattcgacat cttgcaataa atatttcagt tctgcggtta
     5761 acctcgcacc cgcgtaagct ttattgtatt tctgggcgtg aatattatct aatgtagctt
     5821 catggttcca tccaaaagaa ttatcaccat tcattgttac tccatcgtaa gcattacact
     5881 tcgttgctaa cttattgaat gcttgccagt cagagagatc tagtttgtgg atctcaacag
     5941 tacgtctgcc atcaaaatta tgatctcttc cacgatttga cagtaatgta gcgcgtgcgt
     6001 attgatgaaa ttttaatttt gctggtttgt cggtggacga aaaacggggt aaatatgcgg
     6061 ggaatgaata aatagttgtt
//
LOCUS       DAZKHC020000182         5964 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZKHC020000182 DAZKHC020000000
VERSION     DAZKHC020000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.183"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            402..4304
                     /gene="espP"
                     /locus_tag="ACHOSM_RS22940"
                     /old_locus_tag="pgaptmp_004588"
     CDS             402..4304
                     /gene="espP"
                     /locus_tag="ACHOSM_RS22940"
                     /old_locus_tag="pgaptmp_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HGU4560938.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMHFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            4811..5176
                     /locus_tag="ACHOSM_RS22945"
                     /old_locus_tag="pgaptmp_004589"
     CDS             4811..5176
                     /locus_tag="ACHOSM_RS22945"
                     /old_locus_tag="pgaptmp_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560939.1"
                     /translation="MNDGRQTPPSPEAWKIRFLANGTPGTWFYTDDAGVTKEWLRAHC
                     DDVEVIPLCRMHDGPVFREHHINELSAALARMLHMHHILLERTRYRENIHDAECIREI
                     HEAPLQAIGILEKYKRTKS"
     gene            5186..5377
                     /locus_tag="ACHOSM_RS22950"
                     /old_locus_tag="pgaptmp_004590"
     CDS             5186..5377
                     /locus_tag="ACHOSM_RS22950"
                     /old_locus_tag="pgaptmp_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560940.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFTAL
                     KKENVGEFALRGVCVPAGA"
     gene            5405..5716
                     /locus_tag="ACHOSM_RS22955"
                     /old_locus_tag="pgaptmp_004591"
     CDS             5405..5716
                     /locus_tag="ACHOSM_RS22955"
                     /old_locus_tag="pgaptmp_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HGU4560941.1"
                     /translation="MTSPDLIQADLHRMSWSQLARAAEESTVHHDYARALILWRHAYH
                     AATLTINKNLATAKINFCAKRILMRNQMSKIIRHTDTDEILFRLNKHHHLYEKKKTKD
                     G"
     gene            complement(5859..5954)
                     /gene="dqlB"
                     /locus_tag="ACHOSM_RS22960"
                     /old_locus_tag="pgaptmp_004592"
     CDS             complement(5859..5954)
                     /gene="dqlB"
                     /locus_tag="ACHOSM_RS22960"
                     /old_locus_tag="pgaptmp_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HGU4560942.1"
                     /translation="MINLLIIVLRAVVAVVNAVIALLELIRELID"
BASE COUNT         1807 a         1373 c         1326 g         1458 t
ORIGIN      
        1 aaatattgaa tacatttact gagcatgcaa aagaaaaaga caaactaatc ccggcctttc
       61 acagaatttc ctctatcaga ggctgagttg acaacctgct cacccaggaa atagtaacac
      121 acgaaaacat gaagagacac tgataaacaa tctcgataca tattcctcat ctttcacagc
      181 atctcacttc tttaacagga attattatta tctacgcctc ctcctttcat gtatccctca
      241 tgaatgatta agataaacat actgtgtttg ttatccgtat gataacaaac acataaagga
      301 ctaaacattt atctggatga tgattacaat gttcacgact gtcagagaca cacctctgat
      361 acattagaaa aaccaatgtt tcccttaaaa atggagctta tatgaataaa atatactctc
      421 ttaaatacag ccatattaca ggagggttaa tcgctgtttc tgaattatcc ggcagagtat
      481 catcaagagc aactggtaag aaaaaacaca aacgcatact tgcattatgt tttttaggct
      541 tattacaatc ctcatattct tttgcgtcac agatggatat ttcaaatttc tacatccgtg
      601 actatatgga ttttgcacag aacaagggca tatttcaggc tggcgcaaca aatattgaaa
      661 tagtgaagaa agatggctcc accctgaaac taccggaagt accatttcct gacttctcac
      721 cggttgcaaa caaagggtca accacatcta ttggtggtgc atacagtatc acagccacac
      781 acaatacgaa aaaccaccac tcagttgcga cgcaaaactg ggggaacagc acgtacaaac
      841 aaactgactg gaatacttca catcctgatt ttgcagtatc ccgacttgac aagtttgttg
      901 ttgagacccg aggtgcgact gaaggcgcag atatttcgtt atcaaaacag caggcacttg
      961 aacgttacgg ggttaattat aaaggagaaa agaaacttat cgcattcaga gccggctctg
     1021 gtgtggtatc cgttaaaaaa aatggacgca taactccatt taatgaggtt tcttataagc
     1081 cagaaatgtt aaatggctct ttcgttcaca ttgatgactg gagtggatgg ctgatattaa
     1141 ccaacaacca gtttgatgag tttaataaca ttgcctctca gggtgacagc ggttcagcac
     1201 tgttcgtcta tgataaccaa aagaaaaagt gggttgtcgc tggaactgtc tgggggattt
     1261 ataattacgc caatggcaaa aaccacgcag catacagtaa atggaaccag acaaccattg
     1321 acaacctgaa gaacaagtat tcttacaacg tggatatgtc aggagctcag gttgcaacta
     1381 ttgaaaatgg aaaactgaca ggcactggct cagacaccac cgatataaaa aataaggact
     1441 taatatttac tggcggtgga gatatcctcc tgaaatcctc ttttgataat ggtgctggcg
     1501 gtcttgtctt taatgataaa aagacctatc gagtaaacgg ggatgatttc acctttaaag
     1561 gtgccggtgt tgatacaaga aacggcagca ccgttgagtg gaatatccgg tatgataata
     1621 aagacaacct tcacaaaatt ggtgatggca cattagatgt ccgaaaaacc cagaacacca
     1681 acctgaaaac aggtgagggt cttgtcattc ttggagctga aaaaacattc aataatatct
     1741 acataaccag tggtgatgga actgtccgac tgaatgcaga aaatgcactg tctggcggtg
     1801 aatacaacgg tattttcttt gcgaaaaatg gcggaactct tgacctgaac ggatataatc
     1861 agtctttcaa taaaattgct gcaactgatt caggtgctgt aataaccaat acgtcaacca
     1921 aaaaatccat tttatccctg aataatactg ctgactatat ctatcacggc aacataaacg
     1981 ggaatctgga cgtacttcag catcatgaga cgaaaaaaga gaaccgtcgt cttattcttg
     2041 atgggggcgt ggacacaaca aatgatataa gcctgcgtaa tacacaactg tccatgcagg
     2101 gacatgccac tgaacatgcc atttatcggg atggagcttt ctcttgttca ctaccagctc
     2161 ctatgcactt tttgtgtggc agtgattatg ttgcaggaat gcaaaataca gaagctgatg
     2221 ctgtaaaaca aaacggaaat gcctataaaa ccaacaatgc tgtctctgat ttatcgcagc
     2281 cagactggga aaccggaaca ttcagatttg gaacgctaca tcttgaaaat tccgattttt
     2341 ctgttggtcg taatgcaaat gtaatcgggg acattcaggc cagtaaatca aacattacta
     2401 ttggtgacac tacagcatat attgatttgc atgctggtaa aaatattacc ggtgatggtt
     2461 ttggcttccg ccagaatatt gtgcgtggaa actcacaagg agaaacgctg tttacaggag
     2521 ggatcacagc agaagacagc actatcgtta ttaaagataa agcaaaagca ttattttcaa
     2581 attatgtata cttgctgaac acaaaagcaa ccatagagaa cggtgctgat gtgacaactc
     2641 aaagtggtat gttctccacg agcgatatca gcatctctgg taatctgtcc atgacaggca
     2701 atcccgacaa agacaataaa ttcgagccct caatatatct gaatgatgct tcttatctac
     2761 tgactgacga ctccgccaga ctcgttgcca aaaataaagc atctgtggtg ggagatatac
     2821 actccactaa aagtgcatcc atcatgtttg gtcatgatga aagcgacctc tcgcagttgt
     2881 ctgacagaac ctcaaaaggg cttgcacttg gtcttttagg tggctttgat gtctcatatc
     2941 gcggttcagt caatgccccg tcagcatctg ccactatgaa caacacctgg tggcaactaa
     3001 ccggagattc tgcgctgaaa acactgaaaa gtacaaacag catggtctat ttcactgaca
     3061 gcgcaaacaa taagaaattc catacgctga cggtcgatga gctggcaacc agcaacagcg
     3121 cctatgcgat gcgtacaaac ctttctgaat cagacaaact ggaggtcaaa aaacacctgt
     3181 ctggtgagaa caatatttta ctcgttgatt tccttcagaa accaacgcct gaaaaacaac
     3241 tgaatattga actggtaagc gcgccaaaag acaccaatga aaatgtcttt aaagccagta
     3301 aacaaaccat tggtttcagt gatgtaacgc cggtcattac aaccagggaa accgatgaca
     3361 aaataacatg gtcactgaca ggctataaca cggtagcaaa caaggaagca acccggaatg
     3421 ccgccgccct gttctctgtt gactataaag cgtttctgaa cgaggtcaac aacctgaaca
     3481 aacgtatggg tgacctgcgt gatatcaacg gcgaagccgg tgcatgggca cgcatcatga
     3541 gcggtaccgg ctctgccagt ggtggtttca gtgacaacta cacgcacgtt caggtcgggg
     3601 tcgacaaaaa acacgagctg gacggactgg atttgtttac cggtttcact gtcacacaca
     3661 ctgacagcag tgcctccgcc gatgttttca gtggtaaaac gaagtctgtg ggggctggcc
     3721 tgtatgcttc cgccatgttt gattccggtg cctatatcga cctgattggc aagtatgttc
     3781 accatgataa tgagtacact gcaacctttg ccggactcgg aacccgtgat tacagcacgc
     3841 attcatggta tgccggtgca gaagcgggct accgctatca tgtcactgag gatgcctgga
     3901 ttgagccaca ggctgagctg gtttacggtt ctgtatccgg taaacagttt gcatggaagg
     3961 accagggaat gcatctgtcc atgaaggaca aggactacaa tccgctgatt ggccgaacgg
     4021 gtgtggatgt gggtaaatcc ttctctggta aggactggaa agtgacagcc cgtgccggtc
     4081 tgggctacca gttcgacctg ctggctaacg gcgaaaccgt attgcgggat gcatctggtg
     4141 aaaaacgcat caaaggtgaa aaggacagcc gtatgctgat gtccgttggc ctgaatgcag
     4201 aaatcaggga taacgtccgc tttggactgg agtttgagaa atccgccttt ggtaagtaca
     4261 acgttgataa tgcagtcaac gctaatttcc gttactcgtt ctgatactcc gtattcaaaa
     4321 gggcctgcgg gccctttacg ctcagtgcct gccgccctga tatgtctgag agacatttcc
     4381 gggatgtcag tcgactggtt tacttaactg cgatgactaa agctggcagt aatgccggct
     4441 tttttacggc cacatacgtc ttcctctcat ccggaactgt gatacaaatc ctgcatacag
     4501 gattgaccgc cggttcctgc aaatggcaca ttttcagtaa gatagcgaag gttacattcc
     4561 cccgtcatgt gtggcacaag ctgtcaccgg gaaagaaacg gaaagcacat gaaaaacatc
     4621 aaaactcaca ccggactcct tatccacaaa gaacaaacgc ggcgcgttcg tcttcatgaa
     4681 acacccactg cctggtgcca cacgcaccgg gaatgctaca gcaaaacgac cggtcgccgc
     4741 tgcggcagcc ctgacagtct cagcaggctg attctgagca gtattcagcc catagaatga
     4801 gatgacccgt atgaatgatg gcagacagac gcctccgtcc cctgaggcat ggaaaatccg
     4861 gtttctggcg aacggtacac ccggcacatg gttttacacc gatgacgcag gcgtcacaaa
     4921 agagtggctg cgggcacatt gtgacgatgt ggaagtgatc cccctgtgcc gtatgcatga
     4981 cggtcccgtt ttccgggaac accatattaa tgaactcagc gccgcactgg ccagaatgct
     5041 ccatatgcac cacatactgc tggaacgaac ccggtacagg gaaaatatcc atgatgcaga
     5101 atgtattcgt gaaattcacg aagctcccct gcaggctatc ggcatcctgg aaaaatacaa
     5161 acgcacaaaa tcataaggca gttctatgtg gatactgatt ttctggctct catccccctt
     5221 taatgtctcc tccggcgatg cccggcaacc cacactggcc gttcacagtc aggagttccg
     5281 tactgaaaaa gcctgtcggg atgccttcac cgcgctgaaa aaagaaaacg tgggggaatt
     5341 tgctctccgt ggcgtctgcg tccccgccgg agcataaaca agacatgcgc aaccggagtc
     5401 cgctatgaca tccccggacc ttatacaagc tgatctgcac cgaatgtcct ggtctcagct
     5461 ggcaagagcc gcggaagagt cgacagtaca tcatgattat gcccgggcgt taatattgtg
     5521 gcggcatgca tatcatgccg ccacgctgac tattaataaa aacctggcaa cggctaaaat
     5581 caacttctgt gctaagcgca ttctgatgcg taatcaaatg agtaaaataa tcagacatac
     5641 agacacagac gaaatactct tccgtctgaa taaacaccat catttatatg agaaaaagaa
     5701 aacaaaagat ggatgacaat cccatgatat gggaaaaact tgccatgctt actgcaggtg
     5761 catttttgat actggcagtc ctgtgggggt aataatatcc tgtttattaa agggcagaag
     5821 gcggtgcccc tctgccctta agtacgtttc cgggattatc agtcaataag ttcccggatc
     5881 agttccagaa gagctatcac tgcattgacc acagcaacaa ctgccctgag aacgatgatt
     5941 aacaggttaa tcatccgttt ctcc
//
LOCUS       DAZKHC020000183         5911 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZKHC020000183 DAZKHC020000000
VERSION     DAZKHC020000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.184"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            202..336
                     /locus_tag="ACHOSM_RS22965"
                     /old_locus_tag="pgaptmp_004593"
     CDS             202..336
                     /locus_tag="ACHOSM_RS22965"
                     /old_locus_tag="pgaptmp_004593"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560943.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            429..671
                     /locus_tag="ACHOSM_RS22970"
                     /old_locus_tag="pgaptmp_004594"
     CDS             429..671
                     /locus_tag="ACHOSM_RS22970"
                     /old_locus_tag="pgaptmp_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560944.1"
                     /translation="MNGYLTSMAGLLQVLLLFWGGTGVVNIYDARDSHDGPVHQCMRR
                     DIVRVFFRGSGIRMQKIPLLDWFYAINQYDNLLIFY"
     gene            938..1759
                     /locus_tag="ACHOSM_RS22975"
                     /old_locus_tag="pgaptmp_004595"
     CDS             938..1759
                     /locus_tag="ACHOSM_RS22975"
                     /old_locus_tag="pgaptmp_004595"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGU4560945.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            1759..2865
                     /locus_tag="ACHOSM_RS22980"
                     /old_locus_tag="pgaptmp_004596"
     CDS             1759..2865
                     /locus_tag="ACHOSM_RS22980"
                     /old_locus_tag="pgaptmp_004596"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU4560946.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            2955..4676
                     /gene="cptA"
                     /locus_tag="ACHOSM_RS22985"
                     /old_locus_tag="pgaptmp_004597"
     CDS             2955..4676
                     /gene="cptA"
                     /locus_tag="ACHOSM_RS22985"
                     /old_locus_tag="pgaptmp_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU4560947.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            4750..5748
                     /gene="lpxM"
                     /locus_tag="ACHOSM_RS22990"
                     /old_locus_tag="pgaptmp_004598"
                     /gene_synonym="msbB"
     CDS             4750..5748
                     /gene="lpxM"
                     /locus_tag="ACHOSM_RS22990"
                     /old_locus_tag="pgaptmp_004598"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGU4560948.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFSLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
BASE COUNT         1526 a         1279 c         1476 g         1630 t
ORIGIN      
        1 tttcagttca tgaatgttcg caaaaaataa atgaacaata attttatgta tttaaaataa
       61 attaatttta cttctgtaat ggtgtcatga ttagcagctt tttaaactaa tatcgacttt
      121 caataaataa ggacaggtaa tggacaacaa aaatatctga acccgggtga gattcataag
      181 ttgcacagtg ctgtcttcaa agtgccacac ccggaacgta atcactgcct gctccttatg
      241 gggtatcttc atggtgtcca agcatcagag ttattaggta taaaactgtc tgatattgat
      301 ttgcaggccg gtaacctgaa tatccggcgg ctctaaacgg tttgtcaact cagcccctcg
      361 gctgaaacag gaggtacagt ccattcgtcg ctggctgaaa cttcgggatg aatgggtaga
      421 agagggatat gaatggttat ttaacctcta tggcaggtct gctacaggta ttactgttgt
      481 tctggggggg cactggtgtg gttaatatct acgatgcaag agattctcac gatggcccgg
      541 tccatcagtg catgcggcgc gatattgtgc gagtattttt cagggggagt ggtatccgga
      601 tgcagaaaat tccgctactc gactggtttt atgcaataaa tcagtatgat aatcttctga
      661 ttttttactg aggcggatat ctctcgctgt gtttactgaa attgcggtat tctgtttttt
      721 ctgaaaaaca gaaaatctga ttaaaaagag catatgcgtt aagggaaatt taagttctgg
      781 ttgattaact gataaaaata tttaaaaatc cctgttgtcg gtaatctccg gaccattccg
      841 ggattacaca ataatatcac catgtgtgat atctatggat cctcactgtt gtttacgcga
      901 ggatattttc agaatgctta acagacgata attttttatg ttgaatgcaa ggcatcttcc
      961 cgtactgatg tatcatcatg tcagtcgttg tccggggctt gtgacactct cacctgaaac
     1021 attttgtgaa cagatgaagt ggctcgcaga taataactgg cggacggtaa catctgctga
     1081 gcttgaatat ttttatcagg ggggaacgct tccacgaaag agtgtcatgc tcacattcga
     1141 tgatggttat cttgataact ggctcagagc atggccggtc ctgaaggaat ataaccttca
     1201 tgcacatatt tttctcatta cggggctgat tggtaaggga aacgtacgta gcaggcagga
     1261 gcaggaatat tctcaccgcg actgtgaacg gttgattgcc gataaccgtt cagatgaggt
     1321 catgcttcgc tggtctgaag tccgggaaat gcgtgattcc ggactggttg aatttcatgt
     1381 gcacacccat tcacataaac gctgggacag gttgtctgta tcacgtgctg aacagtgccg
     1441 gctcatgaaa gaagatattc tggtgggaaa acaatgtctg acggaaaagc tggggttctg
     1501 tagttcccat ctgtgctggc ctgaaggata ttataatcgg gattatatta atctcgccgg
     1561 taagcttggt ttctcttatt tatatacaac cgaaagaaga atgaactgtc ctgaaaatgg
     1621 ttcattacgc atagggcgta tcagcaccaa agagcgggaa cattccggct ggctgaaacg
     1681 acgccttttt tattatacaa cgccactttt ttcttcagta ctggctcttc ataaggggcc
     1741 aagattacca gataattaat gatgaaaatt ttattcactg aatcttcgtc agatattggg
     1801 ggccaggagc tgcaggcact ggcccagatg actgctcttc aaaagcaggg acattccgtt
     1861 ttacttgcct gcagggaaaa aagtaaaatt gctccggaag ccagaaaaag agggcatgat
     1921 gtcactttta tccctttcag gaacagcctt caccttcctt ccatcctcag acttcgacgg
     1981 attatcgggg aatttaaacc tgatctggtc atctgccata gtggccatga cagtaatata
     2041 gcaggactga gccgtctgat atgctgtcac cgcttcagta ttgtcagaca aaaaacctac
     2101 atcacccgta agacccgaac gttttccctg aattatctgt gtgatttcat tgttgtgcct
     2161 agctcagcca tgatggcgca tctgatggcg gaaggtgtca ggacaccggt aacggttatt
     2221 ccaccgggtt tcgactggcc cgcattgcat aatgaagcca tgcgaccgtt acccctgcat
     2281 atccacgcct gggctgcatc agcagacaat gttccgctta tcgttcaggt ggggatgcta
     2341 cgtcccgaaa aaggacatga atttatgctg cgtgtattgt atcagctgaa gatggaaggg
     2401 aaatcatttc gctggctcgt ggtgggcgcc gggcgtgaag aatacgaagc acgtctgcgt
     2461 cagcagacag aacacctggg aatgagcgga gatgtgttaa tggcaggtgc ccttttccca
     2521 gcgttaccgg tctaccggat tgccagtgtg gtggtcatgc cttcggaaaa tgaagcgttt
     2581 ggtatggtac tggctgaagc gtcagtctct ggcgtgcccg ttatcgccag tgagacgggc
     2641 gggatccctg atgtcattca gaagaatgtg acagggacgc tgttacccgt gggggatgtg
     2701 tctgcatgga ccggcgcact gcgcgatttt ttatcccgac cggaacgctt ccggatgatg
     2761 gcagccagcg ccagagaaga tattgagtac cggtttgata ttaacagaac ggcacagatc
     2821 attgtgtctc tggctagtca ggctaagggg aaatgtaaca gataacggcg gatcctcttt
     2881 tcagcccgaa ttttgttcag cggttttccg gtaacggagt aatttatcac attgtttaaa
     2941 tacggggacg gtcaatgcat ttaaataccg gacagaacag gcctactttc agctggagtg
     3001 cgctgggatg ggcaattttt tattttggct ttttttccac tctcctgcag gtcatcatct
     3061 tcagcagtgg gtacagcgga acgaatggaa tacgggactc actgttattc agttgtctgt
     3121 ggttgatccc ggtgtttctc tatcctgatc ggataaaaat aattgcagct gttgtcggtt
     3181 tcattctctg gggcacgtcg ctggcagcac tttgttatta ttttctctat ggtcatgaat
     3241 tctctcaaag tgttcttttc gttatgtttg aaacgaatgc cagagaggct ggtgaatatt
     3301 tcagccagta tttcagcctt aaactgttgc ttatctcgct ggtatatact gcggtgtccg
     3361 tttttctgtg gacacgtctg cgtcctgtat atattccttt gccatggcgg agaattgtct
     3421 ctttcctgct gctttatgct ctgcttctgc atccggttgt tctgaaatcg ttaatcagac
     3481 aggagccgct gaatgatact ctaggcaaac tggcatcccg aatggagcct gctgccccct
     3541 ggcagtttgt atccagttat tatcagtacc atcagcaact gaatgcactg acaaccttcc
     3601 tgaatgaaaa tagcgcactg ccaccactgg gtaatctcag ggatgaatca ggggagagac
     3661 cacgcacact ggtcctggtg attggtgagt cgacacagcg cgaacgcatg agcttatacg
     3721 ggtatctacg tgaaacgacg ccggagctgg atgcactgcg taaaaccgat ccgggtctta
     3781 ctgtgtttaa taatgtggtg gcatcgcgtc cgtacaccat tgaagcattg caacaggccc
     3841 ttactttcgc caacgaaaag aatcctgatc tgtatctgac gcagccgtcg ctgatgaaca
     3901 tgatgaagca ggcaggctat aaaacattct ggattaccaa ccagcagaca atcacagccc
     3961 gtaacaccat gctcactgta ttttcgcgcc agacggacag gcagtactac atgaatcagc
     4021 agcgaacaca aagtgcgcgt gaatatgaca ctaacgtgct gaagccgttc cgggaagtgc
     4081 tgaatgaccc tgcaccaaag aaactgatca tcgtgcatct gctgggtacg cacattaagt
     4141 ataaataccg ttatccggaa ggtcaggggc ggtttgatgg cattacaggg catattccca
     4201 ctggattaaa tgcgaaagag ctggaagtgt ataacgatta cgataatgcc aatctgttta
     4261 acgatcatgt ggtggccagt ctgataaagg acttcagagc aactgcgccg gacggctttc
     4321 tgctttattt ttcagatcat ggtgaagaag tatatgatac tccgccatat aaaacgcagg
     4381 gacggaatga agataacccc acacgtccca tgtacactgt tccgttcctg ctgtggacct
     4441 cggaaaagtg gcatgctgca catccgcgag atttttcgca gtatgttgac cgcaaataca
     4501 gtctggctga actgatccac acctggtcag atttggcggg actgacatac gatggttacg
     4561 atccgacccg ttctctggtg aatccgcaat tcagggaaac cacccgctgg attggaaatc
     4621 cgtataaaaa gaatgggctc actgatttcg acactcttcc gtatggtgag ccatagtatt
     4681 ttcttatctt cggttccgtt ctgaaggcag gaggatgtgt tttcctctgc tgtccggtgg
     4741 cctccaggga tggtaccacc ttctgctgta ctctgttatc ataatgagat aagcaggcag
     4801 ataccggtaa atatgaaaaa tatcagaact gagtttattc cccgctttag ccttacatta
     4861 tgcttccccc gttactggat gacgtggaca ggtatcggta ttatctgtgt gtttgcaatg
     4921 gtcccccccg cactgagaga tccattgttg ggaaaattgg gcatgctggt tgggcgtctg
     4981 gggaaaagcg cacgtcaacg ggccttgatt aatttatcgc tttgttttcc ggagtacagt
     5041 gataaagaaa aggagaatat cgttgatgcc atgtttgcaa cagcctccat ggccgttgtg
     5101 cttatggctg aattagcact gtccgggcct gataaaattt cgcatcgtat acgctggaat
     5161 ggtctggaga ttgtggagaa gatggcacag aacaatgaaa aggtcatttt tcttgtgcca
     5221 catgcctggg gagtggatat tcccgcaatg ctgatggctg cgtcgggcag aaaaatggca
     5281 gcgatgtttc ataaccagag aaatccggtg gtggattatg tctggaacag tgttcgtcgt
     5341 cgtttcggag gaaaactgca cgcacgtaat gacggtattg cctcctttgt ccgctcagtc
     5401 cggcagggat actggggata ttatcttcct gatcaggatc atggcccgga attcagtgag
     5461 tttgcagatt tttttgcgac gtataaagca actttaccgg ttatcggacg gctcagcagg
     5521 atcagtggtg cacggataat accgcttttc cctgtttatg acggaaagac ccatcatctg
     5581 actattcatg tcagcccgcc acttgctata cgacagaaga gtgatgctca tattgcgcga
     5641 cagataaacg aagtggttga aaatttcgtt cgtccacatc ctgagcagta tacgtggatc
     5701 ctcaagttgc tgaaaacgcg taaagaaggg gaagaggatc cttactgagg caggtttttc
     5761 aggataatga gcttacccag caatagtgga cacacgctaa gtgagtaaac tctcagtcag
     5821 aggtggctca tatgacaaaa aaacagtacc aaccagtaaa aaaccccgta aatagcattc
     5881 gcctgaattt cgcagtgaag ccctgaagct t
//
LOCUS       DAZKHC020000184         5905 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZKHC020000184 DAZKHC020000000
VERSION     DAZKHC020000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.185"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..281)
                     /locus_tag="ACHOSM_RS22995"
                     /old_locus_tag="pgaptmp_004599"
     CDS             complement(<1..281)
                     /locus_tag="ACHOSM_RS22995"
                     /old_locus_tag="pgaptmp_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HGU4560949.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQ"
     gene            585..1517
                     /gene="rfaD"
                     /locus_tag="ACHOSM_RS23000"
                     /old_locus_tag="pgaptmp_004600"
     CDS             585..1517
                     /gene="rfaD"
                     /locus_tag="ACHOSM_RS23000"
                     /old_locus_tag="pgaptmp_004600"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HGU4560950.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1527..2573
                     /gene="rfaF"
                     /locus_tag="ACHOSM_RS23005"
                     /old_locus_tag="pgaptmp_004601"
     CDS             1527..2573
                     /gene="rfaF"
                     /locus_tag="ACHOSM_RS23005"
                     /old_locus_tag="pgaptmp_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HGU4560951.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2577..3569
                     /gene="rfaC"
                     /locus_tag="ACHOSM_RS23010"
                     /old_locus_tag="pgaptmp_004602"
     CDS             2577..3569
                     /gene="rfaC"
                     /locus_tag="ACHOSM_RS23010"
                     /old_locus_tag="pgaptmp_004602"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HGU4560952.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPEIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALEAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3566..4774
                     /gene="rfaL"
                     /locus_tag="ACHOSM_RS23015"
                     /old_locus_tag="pgaptmp_004603"
     CDS             3566..4774
                     /gene="rfaL"
                     /locus_tag="ACHOSM_RS23015"
                     /old_locus_tag="pgaptmp_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HGU4560953.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(4810..>5905)
                     /locus_tag="ACHOSM_RS23020"
                     /old_locus_tag="pgaptmp_004604"
     CDS             complement(4810..>5905)
                     /locus_tag="ACHOSM_RS23020"
                     /old_locus_tag="pgaptmp_004604"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HGU4560954.1"
                     /translation="PRGAAAVETWMYQVAQRTNFPNRIVCIKNPGYSNYTFVNDNCSI
                     HRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNSMKLYTQIRKR
                     APQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPNGIDLETYQ
                     SNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLKLVVIGDYT
                     EMSKSDKGAYQRNVREIAKRLKDLCIMLGSIPPEKMHCYYPLADLVVIPSQFQEPFCM
                     VAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTLANPELTAV
                     AKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT         1638 a         1295 c         1312 g         1660 t
ORIGIN      
        1 ttttgaattt tttcgatacg acttctgata tatctaaatt tcttctttat atcgataacg
       61 ataaccgttg ttggttttcc gttgcgaatc gcttcaaccc ttggtttcaa atcgggagaa
      121 gtgaaaataa tcatctcatt ttcaagtgcg gccagacgct caaagtaact aaaataaaca
      181 tcaactgaac gagctagttt ttcacggaac cctttattag cagtccaatc ccctctacca
      241 atgtcaaaat atgcagtgat aattgtggtg gatgatttca ttactaataa gaccattaat
      301 ataaaaaaag aattatatca taaatattaa cagaatgtat actgtatatt atccattcaa
      361 atgcttccgt tatttatcat acatcggaat attgatacta aagcactatt cacatgcaaa
      421 accaacatcc gccatgaagg actagctaaa acccaaacta gtttgttgca attagcatcc
      481 ttgcacctct atgtaaaggg ctgaagggat tcggatgtga tggtatgatt acagacattc
      541 gtgtctgaga ttgtctctga ctccataatt cgaaggttac agttatgatc atcgttaccg
      601 gcggcgcggg ctttatcggc agcaacatcg ttaaagccct gaatgataaa ggcatcaccg
      661 atattctggt ggtggacaac ctgaaagacg gcaccaagtt tgtgaacctg gtggatctga
      721 atatcgcaga ctatatggat aaggaagact tcctgatcca gattatggct ggcgaagagt
      781 tcggcgatgt cgaagcgatt ttccacgaag gcgcgtgctc ttccaccacc gagtgggacg
      841 gcaagtatat gatggataac aactatcaat actccaagga gctgctgcac tactgcctgg
      901 agcgtgaaat cccgttcctg tacgcttctt ccgcagccac ctacggcgga cgcacctccg
      961 actttattga atcccgcgag tacgaaaaac cgttgaacgt ctacggttac tcaaaattcc
     1021 tgtttgatga atatgttcgc caaatcctgc cggaagcgaa ctcgcagatt gttggcttcc
     1081 gctatttcaa cgtttatgga ccgcgtgaag gccataaagg cagcatggcg agcgtcgctt
     1141 tccatctcaa cacccagctt aataacggtg aatcaccgaa gctgttcgaa ggtagcgaga
     1201 actttaaacg cgacttcgtc tatgttggcg acgtggcaga tgtaaacctg tggttcctgg
     1261 aaaatggcgt ttccggcatc ttcaacctcg gcaccggtcg tgcggaatcc ttccaggctg
     1321 tagctgatgc tacgctggct tatcacaaga aaggccagat cgaatacatt ccgttcccgg
     1381 ataagctgaa aggccgctac caggcgttca ctcaggcaga tctgacaaat ctgcgcgcgg
     1441 cgggttacga caaaccgttc aaaaccgttg ctgaaggtgt aacggaatac atggcctggc
     1501 tgaatcgcga cgcataagag ctctgcatga aaatactagt gatcggcccg tcttgggttg
     1561 gcgacatgat gatgtcgcaa agtctctatc gcacgctcca ggcgcgctat ccccaggcga
     1621 taatcgatgt gatggcaccg gcatggtgcc gtccattatt atcgcggatg ccggaagtta
     1681 acgaagctat ccctatgcct ctcggtcacg gagcgctgga aatcggcgaa cgccgcaaac
     1741 tgggtcatag cctgcgtgaa aagcgctacg accgcgccta cgtcttaccc aactccttca
     1801 aatctgcatt agtgcctttc ttcgcgggta ttcctcatcg caccggctgg cgcggcgaga
     1861 tgcgctacgg tttactcaac gatgtacgcg tgctcgataa agaagcctgg ccgctaatgg
     1921 tggaacgcta tgtcgcgctg gcctatgaca aaggcattat gcgtaccgca caagatctgc
     1981 cgcagccatt gttatggccg cagttgcagg tgagcgaagg tgaaaaatca tatacctgta
     2041 atcaattttc gctttcatca gaacgtccga tgattggctt ttgcccgggt gcggagtttg
     2101 gtccggcaaa acgctggcca cactaccact atgcggagct ggcaaagcag ctgattgatg
     2161 aaggttatca ggtggttctg tttggctctg cgaaagatca tgaagcgggc aatgagattc
     2221 ttgccgcttt gaataccgag cagcaggcat ggtgtcggaa cctggcgggg gaaacacagc
     2281 ttgatcaagc ggttatcctg attgcagcct gtaaagccat tgtcactaac gattctggcc
     2341 tgatgcacgt tgcggcggcg ctcaatcgtc cgctggttgc cctgtatggt ccgagtagcc
     2401 cggacttcac accgccgcta tcccataaag cgcgcgtgat ccgcctgatt accggctatc
     2461 acaaagtgcg taaaggtgac gctgcggagg gttatcacca gagcttgatc gacattactc
     2521 cccagcgcgt actggaagaa ctcaacgcgc tattgttaca agaggaagcc tgacggatgc
     2581 gggttttgat cgttaaaaca tcgtcgatgg gcgatgttct ccatacgttg cccgcactca
     2641 ctgatgccca gcaggcaatc ccagaaatta agtttgactg ggtggtggaa gaagggttcg
     2701 cacagattcc ttcctggcac gctgccgttg agcgagttat tcctgtggca atacgtcgct
     2761 ggcgtaaagc ctggttctcg gcccctataa aagctgaacg caaagcgttt cgtgaagcgc
     2821 tagaagcaga gaactatgac gcagttatcg acgctcaggg gctggtaaaa agcgcggcgc
     2881 tggtgacgcg tctggcgcat ggcgtaaagc atggcatgga ctggcaaacc gctcgcgaac
     2941 ctttagccag cctgttttac aatcgtaagc atcatattgc aaaacagcag cacgccgtag
     3001 aacgcacccg cgaactgttt gccaaaagtt tgggctatag caaaccgcaa acccagggcg
     3061 attatgctat cgcacagcat tttctgacga acctgcctac agatgctggc gaatatgccg
     3121 tatttcttca tgcgacgacc cgtgatgata aacactggcc ggaagaacac tggcgagaat
     3181 tgattggttt actggctgat tcaggaatac ggattaaact tccgtggggc gcgccgcatg
     3241 aggaagaacg ggcgaaacga ctggcggaag gatttgctta tgttgaagta ttgccgaaga
     3301 tgagtctgga aggcgttgcc cgcgtgctgg ccggggctaa atttgtagtg tcggtggata
     3361 cggggttaag ccatttaacg gcggcactgg atagacccaa tatcacggtt tatggaccaa
     3421 ccgatccggg attaattggt gggtatggga agaatcaagt agagtgtcgt tctacaagta
     3481 tgtctcttgc agatttgcca gctcaaaccg tttttcaaaa tttaaatctt gaaataataa
     3541 ccaataagtt gacatcggag ataagatgac ctcaacatta tttttctctc tcgagaaaaa
     3601 aaactggata gcgtactgga acagagctct cgtattctta ttcattacca cctatttttt
     3661 gggtgggatt acaaggtata aacatcttat tgttattctt atgacaataa cgacaatcgt
     3721 ctatctctgc aaacggccaa aacactatct ctcactattt aaaacatttc tttttggtag
     3781 tgttgccata ttaactattg ctgcattgct gtcacttctt caatcccctg atgcaggtgc
     3841 tagcatgaag gaaattttca aagctattat tgagaatact ttactatgca caatagcaat
     3901 accgatcata ttgagagacg agaaaagaga agatgtcgaa aaaatcgttt tcttctcatt
     3961 tattagtgcg ttgggcttac gctgtttttc tgaattgatt acctattata aggactatca
     4021 acaagggata atgccattcg cagattatag acaccgtagc atttctgact cgatggtctt
     4081 tttattccct gcattgttaa atctctggct tatcaaatca gcaaaatacc gcatttcttt
     4141 tgtggttcta agcgttattt ttatttttct gatattagga actttatcca gaggggcctg
     4201 gctttccgtg ttagtcattg gattaatatg gattctgatg tttaaacaat ggaagttact
     4261 attagtagga gtaatggttg ccatcattgc attgtcggtt attttcacac ataaggagat
     4321 gaccgcaaag ctaacgtata aacttcaaca aactaatagt tcttatcgct atgcaaatgg
     4381 tactcaaggc agcgcactcg atctaatatt agaaaatcct gttattggtt atggttacgg
     4441 taacgttgca tataaagatg tctataataa acgtgtcatt gattatccag aatggacctt
     4501 tagacaatca atagggccac ataattttgc gctattcatc tggtttggca ctggtttatt
     4561 agggctggta agtcttatga tgctatactg tgcaatattg aaagagtgta taaaaaatgg
     4621 cgtcaagaat aaatatcgct caccatataa tgcatattat ataatcttac tatcttttat
     4681 aggttatttt gttatccgtg gaaacgtaga acaaattgaa ccaaatttat taggcgttta
     4741 cgccggctta ttattagcga tgaaaaacaa gtaaaaaata aaaaaggctg cataatgcgg
     4801 cctttttatt tattcaaacc aattatgaat aacctcttcg aatctttgag ttacgccatc
     4861 ccaactgtag tgatcaaaca caaagtcctg accttgcttt gcaactgctg ttaattcagg
     4921 attcgctaac gtttttaaaa tatcgctgct tatggagtct gcagtcatag gttcttttaa
     4981 atggaatccc gtagtatttt ctttgacaaa ttccgtcatc cccccacgcg tactcactaa
     5041 aacaggctta ccagcaccca tagcttcaat tgccaccatg caaaatggtt cctggaactg
     5101 ggaaggaatg acgactaaat ctgccagtgg gtaataacaa tgcatctttt caggcggtat
     5161 gcttcctaac ataatgcaaa gatccttcaa acgttttgca atttccctga cattgcgttg
     5221 atatgcacct ttgtcacttt tactcatttc tgtataatca ccaatcacaa ctaattttaa
     5281 atttttgtga gcagcggcca atttttcgaa tgattgcatc aacagcaata tgcctttgtc
     5341 agggacgata cgcccagcat aaaaaataat tttctcttca ggggagatat taagttcgct
     5401 tctagtgatc ggctgaaaat tagattgata ggtttctaaa tcaattccat ttggtacaat
     5461 ctcaatatct gcatttgcta aataagattg gtaatacttt ttcaagtata aactaggcac
     5521 tatcatttta acattttgct caagcaattt tggttcaaat gcattatgca tatgtataac
     5581 cactcttgcc tgaggagctc gcttacgaat ttgagtgtat agcttcatgc tattatgaac
     5641 tacaataaca ctttctttcg ttataggaaa atcatgtgca atattcaata ttctctgtga
     5701 atatggaaga ggatcaagtc ttgtccattt ttgaaataac cgcttatata tgcgactgaa
     5761 tccaacccta tgaatactac aattatcatt aacaaaggtg taatttgagt atcctggatt
     5821 tttgatacaa acaatacgat ttggaaaatt ggtacgctga gcaacctgat acatccacgt
     5881 ttcaacagca gccgctccgc gagga
//
LOCUS       DAZKHC020000185         5887 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZKHC020000185 DAZKHC020000000
VERSION     DAZKHC020000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.186"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            62..1213
                     /gene="yiaY"
                     /locus_tag="ACHOSM_RS23025"
                     /old_locus_tag="pgaptmp_004605"
     CDS             62..1213
                     /gene="yiaY"
                     /locus_tag="ACHOSM_RS23025"
                     /old_locus_tag="pgaptmp_004605"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HGU4560955.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1378..2916
                     /gene="aldB"
                     /locus_tag="ACHOSM_RS23030"
                     /old_locus_tag="pgaptmp_004606"
     CDS             1378..2916
                     /gene="aldB"
                     /locus_tag="ACHOSM_RS23030"
                     /old_locus_tag="pgaptmp_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HGU4560956.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3135..3278)
                     /locus_tag="ACHOSM_RS23035"
                     /old_locus_tag="pgaptmp_004607"
     CDS             complement(3135..3278)
                     /locus_tag="ACHOSM_RS23035"
                     /old_locus_tag="pgaptmp_004607"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560957.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3461..3784
                     /locus_tag="ACHOSM_RS23040"
                     /old_locus_tag="pgaptmp_004608"
     CDS             3461..3784
                     /locus_tag="ACHOSM_RS23040"
                     /old_locus_tag="pgaptmp_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU4560958.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3790..4926
                     /locus_tag="ACHOSM_RS23045"
                     /old_locus_tag="pgaptmp_004609"
     CDS             3790..4926
                     /locus_tag="ACHOSM_RS23045"
                     /old_locus_tag="pgaptmp_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4560959.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4923..>5887)
                     /locus_tag="ACHOSM_RS23050"
                     /old_locus_tag="pgaptmp_004610"
     CDS             complement(4923..>5887)
                     /locus_tag="ACHOSM_RS23050"
                     /old_locus_tag="pgaptmp_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4560960.1"
                     /translation="QLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADFESKV
                     GLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPTGI
                     ITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDESLVA
                     SFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVR
                     DYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLV
                     AIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1452 a         1405 c         1539 g         1491 t
ORIGIN      
        1 gttttcgaat gaaaatcctc agtaagctgc ccaccctttt ttacactttc aggagtgtgt
       61 tatggcatct tcaactttct ttattccttc tgtgaatgtc atcggcgctg attcattgac
      121 tgatgcaatg aatatgatgg cagattatgg atttacccgt accttaattg tcactgacag
      181 tatgttaacg aaattaggta tggcgggcga tgtgcaaaaa gcactggaag aacgcaatat
      241 ttttagcgtt atttatgatg gcacccaacc taaccccacc acggaaaacg tcgccgcagg
      301 tttgaaatta cttaaagaga ataattgcga tagcgtgatc tccttaggcg gtggttctcc
      361 acacgactgc gcaaaaggta ttgcgctggt ggcagccaat ggcggcgata ttcgcgatta
      421 cgaaggcgtt gaccgctctg caaaaccgca gctgccgatg atcgccatca ataccacggc
      481 gggtacggcc tctgaaatga cccgtttctg catcatcact gacgaagcgc gtcatatcaa
      541 aatggcgatt gttgataaac atgtcactcc gctgctttct gtcaatgact cctctctgat
      601 gattggtatg ccgaagtcac tgaccgccgc aacgggtatg gatgccttaa cgcacgctat
      661 cgaagcgtat gtttctattg ccgccacgcc gatcactgac gcttgtgcac tgaaagccgt
      721 gaccatgatt gccgaaaacc tgccgttagc cgttgaagat ggcagtaatg cgaaagcgcg
      781 tgaagcaatg gcttatgccc agttcctcgc cggtatggcg ttcaataatg cttctctggg
      841 ttatgttcat gcgatggcgc accagctggg cggtttctac aacctgccac acggtgtatg
      901 taacgccgtt ttgctgccgc atgttcaggt attcaacagc aaagtcgccg ccgcacgtct
      961 gcgtgactgt gccgctgcaa tgggcgtgaa cgtgacaggt aaaaacgatg cggaaggtgc
     1021 tgaagcctgc attaacgcca tccgtgaact ggcgaagaaa gtggatatcc cggcaggcct
     1081 acgcgacctg aacgtgaaag aagaagattt cgcggttctg gcgactaatg ccctgaaaga
     1141 tgcctgtggt tttactaacc cgatccaggc aactcacgaa gaaattgtgg cgatttatcg
     1201 cgcagcgatg taatcatcat ttccacaacg gctggcaaat tgtcagccgc tttttcaact
     1261 atctctgtaa cccttgcccg taaattcgtg atagccgtcg taaagctgtt accgactggc
     1321 gaagatttcg ccagtcacgt ctacccttgt tatacctcac accgcaagga gacgatcatg
     1381 accaataatc ccccttcagc acagattaag cccggcgagt atggtttccc cctcaagtta
     1441 aaagcccgct atgacaactt tattggcggc gaatgggtag cccctgccga tggtgagtat
     1501 taccagaacc tgacgccggt gaccgggcag ctgctgtgcg aagtggcgtc ttcgggcaaa
     1561 cgagacatcg atctggcgct agatgctgcg cacaaagtga aagataaatg ggcgcacacc
     1621 tcggtgcagg atcgcgcggc gattctgttt aagattgccg atcgaatgga acaaaacctc
     1681 gagctgttag cgacagctga aacctgggat aacggcaaac ccattcgcga aaccagtgct
     1741 gccgacgtgc cgctggcgat tgaccatttt cgctatttcg cctcgtgtat tcgggcgcag
     1801 gaaggtggga tcagtgaagt tgatagcgaa accgtggcct atcatttcca tgaaccgtta
     1861 ggcgtggtgg ggcagattat cccgtggaac ttcccgctgc tgatggcgag ctggaaaatg
     1921 gctcccgcgc tggcggcggg caactgtgtg gtgctgaaac ccgcacgtct taccccgctt
     1981 tctgtactgc tgctaatgga aattgtcggt gatttactgc cgccgggcgt ggtgaacgtg
     2041 gtcaatggcg caggtgggga aattggcgaa tatctggcga cctcgaaacg catcgccaaa
     2101 gtggcgttta ccggctcaac ggaagtgggc caacaaatta tgcaatacgc aacgcaaaac
     2161 attattccgg tgacgctgga gttgggcggt aagtcgccaa atatcttctt tgctgatgtg
     2221 atggatgaag aagatgcctt tttcgataaa gcgctggaag gctttgcact gtttgccttt
     2281 aaccagggcg aagtttgcac ctgtccgagt cgtgctttag tgcaggaatc tatctacgaa
     2341 cgctttatgg aacgcgccat ccgccgtgtc gaaagcattc gtagcggtaa cccgctcgac
     2401 agcgtgacgc aaatgggcgc gcaggtttct cacgggcaac tggaaaccat cctcaactac
     2461 attgatatcg gtaaaaaaga gggcgctgac gtgctcacag gcgggcggcg caagctgctg
     2521 gaaggtgaac tgaaagacgg ctactacctc gaaccgacga ttctgtttgg tcagaacaat
     2581 atgcgggtgt tccaggagga gatttttggc ccggtgctgg cggtgaccac cttcaaaacg
     2641 atggaagaag cgctggagct ggcgaacgat acgcaatatg gcctgggcgc gggcgtctgg
     2701 agccgcaacg gtaatctggc ctataagatg gggcgcggca tacaggctgg gcgcgtgtgg
     2761 accaactgtt atcacgctta cccggcacat gcggcgtttg gtggctacaa acaatcaggt
     2821 atcggtcgcg aaacccacaa gatgatgctg gagcattacc agcaaaccaa gtgcctgctg
     2881 gtgagctact cggataaacc gttggggctg ttctgatata agaagctggt cgcattgggt
     2941 attcattgcc tgatgcgacg cttacgcgtc ttatcatgcc tacgggaacc tgaccgtagg
     3001 ccggataagg cgtacacgcc gcatccggca taaacaaagc gcacgttgtt acaatttacg
     3061 ttttaaacct acgtgcgcaa taacccagac tacgccaaca gaagtgcgtt tctggggcgt
     3121 gttttgtcag gagtttaatc tcgcgtttcg atggtgagcg ggcgaaccag gccgtaaatc
     3181 accattggtg atccgaggcc aatcagcaat aaacggtaga taattgaagg tttaatacgc
     3241 ttaatctgta ctgtggatgt tttactggag ttattcatta gtttggccaa tagttccact
     3301 atttcaatat gataaacctc gtcctgttga aaaaccagac agcacatttc agaaagtata
     3361 taaaaaacaa tatgtcgtta tataaataag gaaattgcac tgcatcatgg catttcataa
     3421 tatgcgttaa ttgttctttt ttatattctt tgagggaaat atgttcctgg actattttgc
     3481 actgggagtg cttatttttg tatttctggt gatattctat gggattatta ttttacatga
     3541 tattccctac ctgattgcta aaaaacgtaa tcatcctcat gccgacgcca ttcatgttgc
     3601 tggttgggtg agtcttttta cgctgcatgt tatctggcca tttttgtgga tttgggccac
     3661 gctttaccgc ccggagcggg gatggggaat gcaaaaccat gactcctccg ttgtgcaact
     3721 acaacagcgt attgccgggc tggagaaaca gctcgccgac atcaaatcct cttctgccga
     3781 gtaatactta tggatttact gattattttg acctatgtgg cttttgcatg ggcaatgttt
     3841 aagatcttca aaattcctgt aaataaatgg accattccca cagcggccct gggtggcata
     3901 tttattgtca gtggtttaat tctgttaatg aactataacc atccgtatac cttcaaagcg
     3961 caaaaagcgg ttatttctat tcctgttgtc ccacaggtga caggcgtggt gatcgaagtg
     4021 acggataaga aaaatacgct gattaaaaaa ggtgaggtgc tatttcgact ggacccgacg
     4081 cgttatcagg cgcgggtgga tcggctgatg gcggatatcg ttaccgcaga acataaacag
     4141 cgggcgttgg gcgcagagtt agatgagatg gcggcgaata ctcagcaggc aaaggccacg
     4201 cgggataaat tcgctaaaga gtatcagcgt tacgcacgcg gcagccaggc gaaagtaaac
     4261 ccgttttcag aacgcgatat tgatgtggcg cggcaaaatt atctggcgca ggaagcctcc
     4321 gtaaagtcat cggcggcgga acaaaaacag atccagagcc agctggatag cctggtgttg
     4381 ggtgaacatt ctcaaatcgc cagcctgaaa gcgcagctcg cggaagcaaa atataacctt
     4441 gagcagacga tagtgcgtgc gccgagcgat ggttatgtga cccaggtgct gattcgtccg
     4501 ggtacctatg ccgcgtcgct gccgctacgt ccggtgatgg tgtttatacc cgatcagaaa
     4561 cgacaaattg tggcgcagtt ccgtcagaac tccttgctgc gactggcccc tggcgatgat
     4621 gcggaagtgg tatttaacgc tctgccaggt aaggtattca gcggcaagct ggcagccatt
     4681 agtccagctg ttcccggcgg agcttatcag tcgaccggca ccttacagac gttaaacaca
     4741 gcgccgggtt cagatggcgt tatcgcgacc attgaactgg atgagcacac tgatttgagc
     4801 gctttaccag acggtattta cgcccaggtg gcggtctact ctgatcattt cagccatgtc
     4861 tcggtgatgc gcaaagtgct gttacgcatg accagttggg tgcattacct ttatctcgat
     4921 cattaatctt cgctttccgc cgcttgttca ggccgcagtt gctgcgactg agcaagctgg
     4981 tggcgaattt cacgcagcat ttcatccagc acatactgga cccgccaggg ctggtattcc
     5041 cgtttacgga agatagccac cagatccagc caccattcat acgggtgctg aaagcagggc
     5101 accagcgtgc cgtcttgcag gtcgctctgc acacttttgt ctggcgcaaa cacaatcccc
     5161 aagtgatttc gtgccagctc cagcgccgat tgcgtgttat cgcaaacgta atttcccttc
     5221 acgcgataat cccgcacctc tttactcccg gcgacgttga agcgccagat attcgcgtcg
     5281 tcaatcatca tcgagtcgat caaaatacag gagtgatgtt cgagttcgtc aggacggcta
     5341 ataggatgtt tctcaagata gcgctggctg gcataagcgg ttacggcata ttgtgtaata
     5401 aaactggcaa ccagcgattc atcttttggt ggagcgtagc tgattaaaac atcacaatca
     5461 tcaggaaatt cgacgccttc ataaaaggca ttacgctcaa gattgcaggt ttttagcgat
     5521 aaggtgatat caccgatatc tttaatttta tcgattacat gtttggataa ataggtaatt
     5581 atgccggttg gggcgtagat ggtgacccga ccacgtttct catgcttata atctgcaata
     5641 aaattattaa gttgctcgtt tctgtccagc atgtcgttga tgtacggtaa cagcgcggtg
     5701 ccaaaaggtg tgagcatcag ctgccgggtg gttcggtcaa agacttttaa acccactttt
     5761 gattcaaaat cagcaagata tttgctgacg ttggcctgtg cgatgccaag tacagtcgcg
     5821 gcatggctga tattttcact ggcagcgatt accgagataa tttttaactc ccggtactta
     5881 agttgta
//
LOCUS       DAZKHC020000186         5871 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZKHC020000186 DAZKHC020000000
VERSION     DAZKHC020000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.187"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            53..613
                     /locus_tag="ACHOSM_RS23055"
                     /old_locus_tag="pgaptmp_004611"
     CDS             53..613
                     /locus_tag="ACHOSM_RS23055"
                     /old_locus_tag="pgaptmp_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HGU4560961.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHKEENI"
     gene            613..1524
                     /locus_tag="ACHOSM_RS23060"
                     /old_locus_tag="pgaptmp_004612"
     CDS             613..1524
                     /locus_tag="ACHOSM_RS23060"
                     /old_locus_tag="pgaptmp_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4560962.1"
                     /translation="MFHVDNNSGVANMPELAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENAETARNNLDVYSRSEGDNRYLRR
                     DQNGADIQDKGTFIDNLGLRETVNKAADALPSAGTAVAANRLATPRNINGVPFDGTLD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEMSWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNAWRTVSSV"
     gene            complement(1559..2080)
                     /locus_tag="ACHOSM_RS23065"
                     /old_locus_tag="pgaptmp_004613"
     CDS             complement(1559..2080)
                     /locus_tag="ACHOSM_RS23065"
                     /old_locus_tag="pgaptmp_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4560963.1"
                     /translation="MQLINVKRYYPKDKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            2437..2622
                     /locus_tag="ACHOSM_RS23070"
                     /old_locus_tag="pgaptmp_004614"
     CDS             2437..2622
                     /locus_tag="ACHOSM_RS23070"
                     /old_locus_tag="pgaptmp_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560964.1"
                     /translation="MFISCVPSKATPLILRAFASLLAATAVPFDGRAFAALFTVSRKP
                     RFQGCRISALKGFSTSF"
     gene            2636..3196
                     /locus_tag="ACHOSM_RS23075"
                     /old_locus_tag="pgaptmp_004615"
     CDS             2636..3196
                     /locus_tag="ACHOSM_RS23075"
                     /old_locus_tag="pgaptmp_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4560965.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            3296..5335
                     /locus_tag="ACHOSM_RS23080"
                     /old_locus_tag="pgaptmp_004616"
     CDS             3296..5335
                     /locus_tag="ACHOSM_RS23080"
                     /old_locus_tag="pgaptmp_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4560966.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHTRPS
                     GNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETYDRPEPRIMQLARRSTVTPGGKA
                     CQYNDIILADHCLHDVQDMSGKNHPKADVAKGQYGTVGQGLHIAKKLLPFIPADAGIL
                     LVPCCRGGSAFTAGADGTYSDSTGASEDSARWGVDKPLYKDLISRTKAALAKNPKNRL
                     LAVVWMQGEFDIDAKPTEHSALFLAMVEKFRADLAEQAEQCTGGSAAGVPWICGDTTY
                     FWKQKNEPAYQAIYGGYKNKTDKNIHFVPLMTDENDANVPTNNPAEDPDIESIGYYGS
                     TWRNSAATWTSADRASHFSSWARRGIISDRLATAVLTHAGRTTVKADVPSSETEAPVP
                     SPSETEAVTTTLLSYRVSESEGNLKAQGWEPAGGKAEIISDAGGTGGKAMKLTKETGK
                     SSWYLDHDAGTGAELLKNGGLISCRFKVPGDLVANQYVMALYWPVSSLPQGVTLTGDA
                     GNNLLASFYIQTDAKDLNVMYHNAKVATNNQKLGTFGAFDNEWHTLAFRFAGNNSLQV
                     IPVIDGQDGAAFTLTQSPVGTFPVDKLRVTDITKNATYPVLIDSIVVEVKKAVTE"
     gene            5484..5666
                     /locus_tag="ACHOSM_RS23085"
                     /old_locus_tag="pgaptmp_004617"
     CDS             5484..5666
                     /locus_tag="ACHOSM_RS23085"
                     /old_locus_tag="pgaptmp_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4560967.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDPTPL"
     gene            5702..>5871
                     /locus_tag="ACHOSM_RS23090"
                     /old_locus_tag="pgaptmp_004618"
     CDS             5702..>5871
                     /locus_tag="ACHOSM_RS23090"
                     /old_locus_tag="pgaptmp_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4560968.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGHSPAE"
BASE COUNT         1544 a         1378 c         1505 g         1444 t
ORIGIN      
        1 tgaagtccgt gatggccgga gatacagagt tgctgaccgt gggggaaatc acatggctgt
       61 aaccctgacc ccgcatcagc gcgccctgtt gcagttgctg cctgacgggc tggcatggga
      121 taagcggccg tcatccgttc ttgcggcttt gtgcctgggc ctcagtcatt ccacggagcg
      181 tgtttcctgg accggtaacc agatgctggc agaacgtttt cctgattcat cccgtctgct
      241 gctggaagac tgggagcgtt atctggggtt accggaatgt gatatgaccg gcgcaaccat
      301 tcaggaacgt cagcgttatg cagggaataa ataccggatg aaaccctctc ttaaccgtga
      361 attttatatc cggtttgcgg cagagtttgg ttatgaaata gatattcagc catcaccgga
      421 ttcacagtgg gtcagtattg tcacgataaa cagtgaaacc ggctaccgga atatgaatgt
      481 gctggatgat attctcaccc cgctgcgtat ttatgagggt ggtgcgctgg aatgtattct
      541 gaaccgttat aagcctgcat ggcagacgtt tatttacgtg tatgcaaaca gtcacaaaga
      601 ggagaatatt taatgttcca tgttgataat aattccggcg tggcgaatat gcctgaactg
      661 gctccggcgc agagtaatac caccacctgg tttaccgaag gtgacggaca aaaaggtatc
      721 agctggattg gtcaggactg gctgaatatt ctccaggccg aactgctgaa tattctggct
      781 gaagccagta ttcagccgga taaggcgcag ttaaaccagc ttacgctgtc cattaaagcc
      841 attatcgctg cgaatgcctt ttcccggaaa aacaacctga aagaaattgc tgatgccggt
      901 gcggaagccc agcgtctggc ccgtggttat cttggtctgg ggacgcttgc cacaaaaaac
      961 agtcttggtc ccgctgacgt taatgccctg gcgaaggatc agaaccttgc cgacctggag
     1021 aatgcggaaa ccgcccgtaa taatctggat gtttacagca ggagcgaagg tgataaccgt
     1081 tacctgcgca gggaccagaa tggcgcggat atccaggaca aaggaacctt tatcgataac
     1141 ctcggtttac gggaaacggt aaataaggct gctgatgccc tgccatcggc tggaaccgcc
     1201 gtggcagcaa acagactcgc caccccaaga aatattaatg gtgttccttt tgacggaacg
     1261 ctggatatca acatcacgtc aggaatgacg cagtcaaccg cagatggccg gtatgtacag
     1321 aatgttcagc ttggagcaca gagctatcat tcacccggag gcaatgaaat gtcatggaat
     1381 tacagcgcac cttccggttg tatgctttcc ggtattaacg tgcaggaaac cggctcccgg
     1441 tctgcggaca atatcggtgg ggtctattat cgcccggttc agatttatat taataatgcc
     1501 tggagaacgg taagttcagt gtaaaccaca gaaaagggtg ctgaatgcac cctctgaatt
     1561 attccggttt ttccggccag ggaatcgcct ggtaatgccc ctcactggca atatcattaa
     1621 agtgcatttc tttaagctgt cggatatacg ccatccagcg cgtcagctgt aatttatttt
     1681 catcgctaat catgtccagt tttaattctg ttttccagtc atccacggtt tcgttggctt
     1741 cctggagcag cgatgagcga cgcttttcag caaccgtctt ccagtttacc ggcacctttg
     1801 atatcaggcc atcagaaaac tgccagttac cgtcaatatt cacaccttcc ggcagttcgt
     1861 ccacttcaac aacagtaaaa cctgccggat ataaagcaga gacatcttca gcaacagagc
     1921 agataatacc cgttacaggc gaaatacaga gtttgtattt ctttgtaaaa agcgggatag
     1981 attcataaaa atcccttccg tcctcactct ggaaatactg aacatcctca ccatagggtt
     2041 tgtctttcgg gtaatatcgt ttgacattaa tcagttgcat aaacatcacc atgtaaatca
     2101 atgataaaaa caggaataat actatcgcca tatacaggca atgcttgtct gaaataacgc
     2161 atatttcaga tgctggctgc tgtgtgctat gcccccgtta attaaatact tgcagcggat
     2221 acccaatgcc cattaataca atattgtatc tgtctgtaat aaacacggtc atcaccacca
     2281 cgcatttccc agtctccgtc agtcttaaaa ccggtaatca catttcctga tgacagggag
     2341 taattatatt ccagtccgcc gcttaatgcc tgcgctccca gtcttacccc tgtaacataa
     2401 cgctgattgg ccgttgattc cggcattcct gacgtaatgt ttatatcctg cgtgccgtca
     2461 aaggcgacac cattgattct gcgggcattt gccagcctgc ttgctgcaac agccgtaccg
     2521 tttgatggca gggcattcgc ggccctgttt accgtatccc gtaaaccgag gtttcaggga
     2581 tgccgtatat ctgctttaaa aggcttttcc acctcttttt aaggaaggta aatacatgct
     2641 gattggctat atacgcgtat caacaaatga ccagaacacc ggtttacaaa gaaatgcgct
     2701 ggaatgtgca ggatgtgagc agatttttga agacaaaatg agtggtaaaa cgacacagcg
     2761 gccgggactg aagcgggcac tgaagaaact aaaacccggt gacacgctga tggtctggaa
     2821 actggacagg ctggggcgca gtatgcgcca ccttgttgcg ctgacagatg aattacagca
     2881 acaggggata aattttcgta gtcttactga caacattgat acgtcaaccc ccatggggcg
     2941 cttctttttt cacataatgg gtgcgctggc agaaatggag cgtgaattaa ttgtcgaacg
     3001 gacacgggct ggtcttgcag ctgcccgtga aaggggacga accggcggca gaccccgccg
     3061 tctcacgcag gaacaatacg agcaggtcgg gcgtttactg gccgggggtg tcagtcgaag
     3121 acaggtggca cttatttttg atatcggtct ttcaaccctt tatcgttatt atcctgcgag
     3181 cgtttcggaa aaataagacg ctctctttgt tgggccttta ctgaattaac gcaaaatggc
     3241 gtttatcggt ctgtggatat gataattcag tttattcagg aacaggctga ttattatgac
     3301 gtccaaatgg gttcaactat catcaatgcc cggaaacttt actgttaaag tttccggtgg
     3361 tacagcggca tttcttgagg ctccctttcc tccggccgaa acaaaaggag gaatgacatt
     3421 tgctgactgt ctcatcagtt ttaatacacg ggattgcctg tgggtaaggc cagtatccgg
     3481 tgatccgagt gtggaaatta ccggagcggg tattggtgcg gtcattccgt taagtgctga
     3541 tgttgccggt actgctgaac cgtcagactg ggataatgct gaaacacata cccgtccgtc
     3601 aggaaatgaa actgcttcca gctccccttc ctggtattac gtggtggttc ttgctggtca
     3661 gtcaaacggt atggcttacg gtgaaggtct gccactgccg gagacatatg accgccccga
     3721 accgcgtatt atgcagttag cccgtcgcag tacagtcacg ccgggaggta aagcctgtca
     3781 gtataacgac atcattctgg ccgaccactg tctgcatgat gtgcaggata tgagtggaaa
     3841 aaatcacccg aaagcggatg tggctaaagg gcaatatggc actgtgggtc aggggcttca
     3901 catcgccaaa aaactgctgc cgtttattcc ggcagatgcg gggattctgc tggttccctg
     3961 ctgccgtggt ggttctgcat tcacggcggg tgctgacggc acgtacagtg acagcacggg
     4021 tgcttcagag gactcagccc gctggggtgt ggataagccg ctgtataagg acttaatcag
     4081 ccggacaaaa gcggcactgg cgaagaaccc gaaaaaccgc ctgcttgctg tggtgtggat
     4141 gcagggcgag tttgatattg atgcgaagcc gacggagcat tctgcgctgt ttctggcgat
     4201 ggtggaaaaa ttccgcgcag acctggctga acaggcggaa cagtgtaccg gtggcagtgc
     4261 tgctggcgtt ccgtggattt gtggcgatac cacgtatttc tggaaacaga aaaatgaacc
     4321 ggcataccag gcaatctatg gcggctacaa aaacaaaaca gataaaaaca ttcatttcgt
     4381 tccattaatg acagatgaaa atgacgcgaa tgtacccacc aacaatccgg cagaagaccc
     4441 ggatattgaa tccattggtt attacggctc aacgtggcgt aacagtgccg ccacctggac
     4501 atctgcggac cgggccagcc atttcagttc atgggcacgt cgtgggatta tttccgaccg
     4561 tctggccacg gcagttctga ctcatgcggg gcgaaccaca gtaaaagccg atgttccatc
     4621 ttctgaaact gaagcaccag tgccgtcacc ttcagaaact gaagcagtaa ccacaacact
     4681 gctgtcttac cgtgtcagtg agtcagaagg aaatctgaaa gcccagggct gggagccggc
     4741 tggcggtaaa gctgaaatta tcagtgatgc aggaggcaca ggtggtaagg caatgaaact
     4801 gaccaaggaa acaggtaaat catcctggta tctggatcat gatgccggta ctggtgctga
     4861 actgctgaaa aatggtggtt taatcagttg tcgttttaaa gtacctggcg acctggtggc
     4921 aaaccagtat gtcatggcgc tttactggcc ggtttcctct ctgccgcagg gtgtcaccct
     4981 gacaggggat gcagggaata atctgctggc atcgttctac atccagacag acgcaaaaga
     5041 cctgaatgtg atgtaccaca atgcaaaagt ggcaacaaat aaccagaaac tgggaacctt
     5101 tggtgcattt gataatgaat ggcatacgct ggccttccgt tttgcaggaa ataacagcct
     5161 tcaggttatt ccggttattg atggtcagga tggtgccgcg tttaccctga cgcagtcacc
     5221 agtaggaact ttcccggtgg acaaactccg cgtaacggat atcaccaaaa atgcaactta
     5281 cccggtgctg attgacagca ttgtggtgga agtgaaaaaa gcggtaacag aataaaaaaa
     5341 tcccgccggt catgatatgg ctggcgggag ctacctaacc cataaaggaa aacaaaaaca
     5401 ctcccgaagg agtagttatc agtcacttcg tcatgttttt acccgataac gaattatgta
     5461 gtcaatatca aaggtaaggt tatatgacat tcgttcatac aatgctgctt tatttctgcg
     5521 ctgtggtcag tgcgttatat ctggtgagcg gcggttataa ggtcatcaga aattatattc
     5581 gccgtaaaat tgatgatgcg gcagctgaaa aactcagtaa aacagcacag gcaccgtcat
     5641 ccccaaacga cccgaccccg ctctgataac ggggcaatat acctgcaaaa ggagaatatt
     5701 catgccagag attaaaggca cggttactga agagcttgtc aaacaggcac tttattctga
     5761 agaagtgaac cgcgtgctga aggcgcaggt tcgcaaggat tttgaggcac aaatcgacgc
     5821 atatgttgat gaagtgctgg cccgaatggt tggccattct ccggctgaaa a
//
LOCUS       DAZKHC020000187         5830 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZKHC020000187 DAZKHC020000000
VERSION     DAZKHC020000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.188"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..236
                     /locus_tag="ACHOSM_RS23095"
                     /old_locus_tag="pgaptmp_004619"
     CDS             <1..236
                     /locus_tag="ACHOSM_RS23095"
                     /old_locus_tag="pgaptmp_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4560969.1"
                     /translation="RDGIGYQFDLLANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNIRFGLEFEKSASGKYNVDNAVNANFRYSF"
     gene            complement(284..901)
                     /gene="ureG"
                     /locus_tag="ACHOSM_RS23100"
                     /old_locus_tag="pgaptmp_004620"
     CDS             complement(284..901)
                     /gene="ureG"
                     /locus_tag="ACHOSM_RS23100"
                     /old_locus_tag="pgaptmp_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HGU4560970.1"
                     /translation="MNIIKQPLRVGVGSPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(913..1587)
                     /locus_tag="ACHOSM_RS23105"
                     /old_locus_tag="pgaptmp_004621"
     CDS             complement(913..1587)
                     /locus_tag="ACHOSM_RS23105"
                     /old_locus_tag="pgaptmp_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HGU4560971.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GTAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(1588..2052)
                     /gene="ureE"
                     /locus_tag="ACHOSM_RS23110"
                     /old_locus_tag="pgaptmp_004622"
     CDS             complement(1588..2052)
                     /gene="ureE"
                     /locus_tag="ACHOSM_RS23110"
                     /old_locus_tag="pgaptmp_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HGU4560972.1"
                     /translation="MLYLTRRVEPPAQTTASVTLPVDMRVKSRIKVTLNDGRQTGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDYAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(2062..3765)
                     /gene="ureC"
                     /locus_tag="ACHOSM_RS23115"
                     /old_locus_tag="pgaptmp_004623"
     CDS             complement(2062..3765)
                     /gene="ureC"
                     /locus_tag="ACHOSM_RS23115"
                     /old_locus_tag="pgaptmp_004623"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HGU4560973.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNGSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(3758..4078)
                     /locus_tag="ACHOSM_RS23120"
                     /old_locus_tag="pgaptmp_004624"
     CDS             complement(3758..4078)
                     /locus_tag="ACHOSM_RS23120"
                     /old_locus_tag="pgaptmp_004624"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HGU4560974.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4087..4389)
                     /locus_tag="ACHOSM_RS23125"
                     /old_locus_tag="pgaptmp_004625"
     CDS             complement(4087..4389)
                     /locus_tag="ACHOSM_RS23125"
                     /old_locus_tag="pgaptmp_004625"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HGU4560975.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(4399..5178)
                     /locus_tag="ACHOSM_RS23130"
                     /old_locus_tag="pgaptmp_004626"
     CDS             complement(4399..5178)
                     /locus_tag="ACHOSM_RS23130"
                     /old_locus_tag="pgaptmp_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HGU4560976.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGPLTVQRPFYPEEKTCHLCLLHP
                     PGGIVGGDKLIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYPGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQQVMRDIWQFMRPQLTDKSPVLPRIWLT"
     gene            5559..>5830
                     /locus_tag="ACHOSM_RS23135"
                     /old_locus_tag="pgaptmp_004627"
     CDS             5559..>5830
                     /locus_tag="ACHOSM_RS23135"
                     /old_locus_tag="pgaptmp_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560977.1"
                     /translation="MTEHRSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIL"
BASE COUNT         1305 a         1519 c         1584 g         1422 t
ORIGIN      
        1 cccgcgacgg tatcggttat cagtttgacc tgctggctaa cggtgaaacc gtactgcgtg
       61 atgcatccgg cgaaaaacgc atcaagggtg aaaaagacgg tcgtatgctc atgaatgttg
      121 gcctgaatgc ggaaattcgc gacaacatcc gcttcggtct ggagtttgaa aaatcggcat
      181 ctggtaagta caacgtggat aacgcggtga acgccaactt ccgttattcc ttctgataaa
      241 aacaaggggc cgcctgccgg ccctgataca gaggatgttg tgattactta cccagcatgc
      301 cctgctcttc gataaaggcg atgatgtttt gcagaccttc accacttttc aggttagtga
      361 acccccatgg gcgttctccg cgcatacgta aagtatcacg ctccattacc tcgagggagg
      421 cgcccacgta tggtgcaagg tcagttttat tgatcacgag gaagtcggat ttggtgatcc
      481 ccggaccgcc tttacgcggg atcttttctc cttctgccac atctatgaca tatatggtaa
      541 ggtccgccag ttcggggctg aaggtcgcac tcaggttgtc gccgccgctt tccacaaaga
      601 tcagatcgag attaccaaat ttctcactca gtgcttccac ggctgccaga ttcattgatg
      661 cgtcttcacg aatggctgta tgagggcagc cccccgtttc gacaccgaca atgcgttccg
      721 cttccagtgc tccggcctca gtgagaatgc gttgatcttc tctggtgtag atgtcgttgg
      781 tgactacggc taattgccag gtgtcacgca tggatttgca gagggcttcc agaagcgcgg
      841 ttttacctga gccgaccgga ctgccgacgc cgacacgcag cggttgcttg attatgttca
      901 ttttattttc tcctaagaac ggaataatcg ggaatactgg gtttcatgtc gcgcggaagc
      961 gatagatgca agtggcgttg ctgaaccaat tgcgtgatcc ggggttgcaa gggcactgtc
     1021 catgttcgcc gcatagcggg cacacaggcg cataattaac tgctgggcag ccagctggcc
     1081 gtagggtacc agcctgacgc ccgccatcac tgcgctttca atccagctgt agccaaggct
     1141 cagggccagg tctgaaacgg atatttgcca gcgtacgccg agccatgcca taccggttaa
     1201 ctggctttgc tggcatagtt tgcgccattc ggccgggcag tcgggctgcc agtcgaccag
     1261 aagtctggtg aaggctgtac cgcggttacg ttcttcatct cgtagctcac gggtttccct
     1321 gcaggccagc agataagcgg tccagcgccc ggcactgtca ggatcgcccg cttcacaggc
     1381 ccggtatagc cgggcaagca gtggcaggtc gacggcaaaa aaactctgtt ccatctgcaa
     1441 ctgctgccag tgttcaaagg cggcgctatc ttctacccag cctgcttcca ctgcccactc
     1501 cagtccctgg gaccagctgt agcctccaac agggaggctg ttactggcaa gctgcatcag
     1561 ccgcagacgt ttttccggag tcggcattca gtggctgtgc tcctgatcgt gattatgggc
     1621 gtgcgacttg ctggcgtaag cgccggcttc cggctcaaac ggaagatgtg cataatcaac
     1681 atccagccca aactggcgca gcatatcatc gaggacgtga tcgtgatggt agcgcagctc
     1741 tccgggcatt atttgcagcg gaacgtggcg attgccgaga tgccagcagg ctttcgcgag
     1801 cataaaagga tctgcgcagc gcactacaga aacggcttca tcagcggcga tcaccttaat
     1861 gaattcatca ccgttttcgt tactgagaat atcaccatcg cgcagcagca gaccgcgtgg
     1921 cagcaacagt ccagtctgac gtccatcgtt aagggtgact ttaatacggc tttttacccg
     1981 catgtcaaca gggagggtaa cgctggcagt tgtctgagcc gggggctcca cccgccgggt
     2041 caaataaagc atttgtgctc cttaaaacag gaaataacgt tgcgccattg gcagaatgtc
     2101 cgccggttca ctggttatca gttcgccgtt gatacgcact tcgtaggttt gtgaatccac
     2161 ggttatatca ggcaacaggc tgttgtggcg catatcggct ttttgtactg tgcggcagcc
     2221 ttttaccaca gcagttgtgc tgtgcaggtt aagctgttca gcgacgccat ttgctgctgc
     2281 tgcctgcgac aggaaagtca cacgacagcg gtgacgggca ctgcccaatg cagcgaacat
     2341 tgggcgatag tgcaccggct gcggtgtggg gatagagccg ttgatatcac ccatcggcgc
     2401 catggctatc attccgcctt tgacgatagt cgccggtttt acgccaaaga acgccgggga
     2461 ccagagcacc agatccgcca gttttcccac ttcaatcgag ccgacttcat gagcaatacc
     2521 gtgggttaat gccggattaa tggtgtattt agcgatatag cgcttcacgc ggacgttgtc
     2581 gttatcacca ctttcttccg gtaacgggcc gcgctgaact ttcatccggt gtgccacctg
     2641 ccaggttcgt aacactactt ctccgacgcg tcccatggcc tgcgaatcgg acgaggtgag
     2701 ggagaacgcg ccaaggtcgt gcaggacgtc ttccgcggca atggtttcct ggcgaatgcg
     2761 cgattcggca aaggctacgt cctcggcgat atccgggtca agatgatggc aaaccatcag
     2821 catgtccaga tgctcatcaa tagtgttgac ggtatagggt agcgtcggat tggttgagga
     2881 aggcagaata ttggggtgcg cgcaggcggt gataatatcc ggagcatgac caccacctgc
     2941 accttctgta tggaaggtat ggatagtgcg cccgccgatg gcagtcagag tatcctcaac
     3001 gaatcctgat tcgttaagcg tatcactgtg tagcgcaacc tgaacgtcca tttcgtcggc
     3061 tacagtcagt gcgcagttga ttaccgcagg tgtagctccc cagtcttcgt gaattttgag
     3121 gccgataacc ccggccgcga cctgctcacg aagcgcatcc ggattggagc cattgccttt
     3181 acccagcaac ccgatattga ccggcaggct gtctgcagcc tgcagcattt gataaatgta
     3241 ccatggtcct ggggtacagg ttgtggcgtt agaacccgct gtcgggccag taccgccacc
     3301 gatcatggtg gtaatgccgg atgtcagcgc ttcttcagcc tgctgtgggc agatccagtg
     3361 aatatgcgta tcgacgccac ctgcggtaac gatcctgcct tctgcggcaa taatttccgt
     3421 ggatacgccg attgggatag tgacgttggg ttgtatatca ggattaccgg ctttgccgat
     3481 agcaaatatc cttccatctt tgacgccgat atcggcttta acgatccccc agtaatcgat
     3541 gatcagggca ttggtcagca ccagatcagc gcagccggcg gagagcattt gcccctgtcc
     3601 cataccgtcg cggattactt taccgccgcc gaatttgacc tcttcgccgt aggtagttaa
     3661 atcatcttcg acctcgatcc acagctcagt gtctgccaga cgaattttat caccggtggt
     3721 agggccgaac atgtcagcat aggcctggcg tgaaatatta ctcatcattt gcctccagct
     3781 cacccattat atctccgcgg aaaccgtgca cgatacgggc accgctcagc gccaccagtt
     3841 caacctcacg cttttggcct ggttcgaagc gcactgcagt tccggcggcg atattgagac
     3901 gatagcctct tgccttctgg cgatcgaact tcagtgccgg gttaacctcc gcgaagtgat
     3961 aatgggaacc gacctgaatt ggtcggtctc cgtgattttc aacgataata ctgcaggtag
     4021 cccggccgat gttaagctct atatagccag gtttaacctt atattctcca gggatcatct
     4081 gttacctcag attatcggat tatggacggt aaccagcttg gagccgtccg gaaaggtggc
     4141 ttcgacctgg atatcgggga tcatttctgg tatgccttcc atgacctgct cgcgactcag
     4201 gacatgccgt ccttcttcca tcagcgcagc gacgcttttg ccgtcgcgag cgccctccat
     4261 tataaaagcg ctaatcaggg ccacggattc gggatagtta agtttcaggc cgcgggccag
     4321 acgacgctct gccagcagtg cagcggtaaa cagtaatagt ttgtcttttt ctcttggagt
     4381 cagttccata gcgttctctt aagtcagcca gatccgggga agtaccggtg atttgtcagt
     4441 cagttgcggc cgcataaatt gccagatatc acgcattacc tgctggcaac gcagattatc
     4501 atcacttaaa aagcgaactg tcagtagacc atcggtgagc gtggctccgg cgtagttttc
     4561 cagtggtgtg agtttttcgc gtatgtcttc cagttgcgtt tcattccccg ggtagaacag
     4621 catcgttccc acccatggtt tccgggcaac gcaggtaagt tctccattgg ccacgtgcag
     4681 acgctcaatc aacagcggag aaccgtcgac ccatatctcc agacggttag cgagtgttcc
     4741 gtggctgaag gtttctccga tgaccggacg tccaaggcac agaagatccc aggccagcag
     4801 tgtactggag gacgacagat gaaacacggt atgcaacgtg gcgtgagcgc cgggaaaaaa
     4861 aatcgaatcc tgtggcaacc attccagcgt tgcgttattg cacagcgtca ggttttgctg
     4921 aaggtgcgcc tgcgcgccgc tgctgcggta aaatttactg gcaccaggca tggtgatcag
     4981 cgtatggcaa tcagagtcaa ttattgcgtt gatgatgagt ttgtcaccgc cgacgatacc
     5041 accgggtgga tgaagcagac agaggtggca ggttttttct tcgggataaa aaggacgctg
     5101 aacagtgagc ggaccgacat gatgacgggt ggtgagcgtg gttttcccgt gcagacgctg
     5161 aaaacggaga tccagcatgg cctgccagcc ttttttattt tgtggtaacg cggtactgag
     5221 caatcgtacg cccctgataa ataaagataa aacgacagta aagagccgga tttccttgtt
     5281 atataaaaga tgatgccttt ccagtgagtt ttcgtctgaa taaaacgaat gtttgtttcc
     5341 gtcatgaagc gttccggctc ccggggctat acatcgccag aaaagcaatg catattgtcg
     5401 ggattatcca gagaatcagg cagaaagatc cgacaatggg ctgtgattat gccatctggc
     5461 tcagaatgat atcaaggtgt tatggggata acttgctgat acaatagcag tatgacagat
     5521 tcattaatgc ggaaactgct cactgaaaca gagtgttcat gactgagcac cggtcgggtc
     5581 atgcagatga taatttcccc ttttcttggg gaagcgacag tattttcatg catgcctgtc
     5641 ttcagtcata cggggagagc atctgtgata cagtccatac atttgttgcg gacgtcgctc
     5701 ctgagtcact gactggtctg caaacagttt atctgtacac ggcagggctt ctgtgtgata
     5761 tcgtttttca tatcgagggg gggcctccag agatgaatgg aataataata tttcctgatg
     5821 atataatact
//
LOCUS       DAZKHC020000188         5749 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZKHC020000188 DAZKHC020000000
VERSION     DAZKHC020000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.189"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(239..934)
                     /locus_tag="ACHOSM_RS23140"
                     /old_locus_tag="pgaptmp_004628"
     CDS             complement(239..934)
                     /locus_tag="ACHOSM_RS23140"
                     /old_locus_tag="pgaptmp_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HGU4560978.1"
                     /translation="MDGFSTERNNKDMKMKWYELARSRMKELGITQEKLAEELGMTQG
                     GIGHWLRGSRHPSLDEIGVVFKYLGIDNVSFNHDGTFSPVGEYSSAPVKKQYEYPVFS
                     HVQAGMFSPELRTFTKGDAERWVSTTKKASDCAFWLEVEGNSMTAPTGSKPSFPDGML
                     ILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNDSCSVVG
                     KVIASQWPEETFS"
     gene            1010..1225
                     /locus_tag="ACHOSM_RS23145"
                     /old_locus_tag="pgaptmp_004629"
     CDS             1010..1225
                     /locus_tag="ACHOSM_RS23145"
                     /old_locus_tag="pgaptmp_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4560979.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVACLNKLGAKVSLDDVFPPEHKAA"
     gene            1345..1638
                     /locus_tag="ACHOSM_RS23150"
                     /old_locus_tag="pgaptmp_004630"
     CDS             1345..1638
                     /locus_tag="ACHOSM_RS23150"
                     /old_locus_tag="pgaptmp_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HGU4560980.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVAAILTNKKRPAATERSEQIQMEF"
     gene            1992..2879
                     /locus_tag="ACHOSM_RS23155"
                     /old_locus_tag="pgaptmp_004631"
     CDS             1992..2879
                     /locus_tag="ACHOSM_RS23155"
                     /old_locus_tag="pgaptmp_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU4560981.1"
                     /translation="MENQKTGYIPLYRSILKQSWAKDVYLRTLWENLLLNAARKPYKA
                     NFKGHEWHLQPGQLVVTAADLGLQLCDRHGKPASRDQVERMLQVFVKEGMITIDGEKQ
                     KGRVITITNYHEYAQKMDDSPAHEAAQTTAHDAAHDEASNGAAFRVHAAHESAHEAAQ
                     TTAHHEQEGINKNINNTPLPPNGGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPH
                     AVAVNEKRKRRLKKIIPQLKTPNVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLL
                     REDSLTGVREGKFADRGIA"
     gene            2876..4252
                     /locus_tag="ACHOSM_RS23160"
                     /old_locus_tag="pgaptmp_004632"
     CDS             2876..4252
                     /locus_tag="ACHOSM_RS23160"
                     /old_locus_tag="pgaptmp_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU4560982.1"
                     /translation="MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQRGTIDTSSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTTSNAPAARGR
                     RYAQGADV"
     gene            4249..4518
                     /locus_tag="ACHOSM_RS23165"
                     /old_locus_tag="pgaptmp_004633"
     CDS             4249..4518
                     /locus_tag="ACHOSM_RS23165"
                     /old_locus_tag="pgaptmp_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560983.1"
                     /translation="MNKKQLAILEKAWDAQISCALKEQALPIIQTKSKIARQLCDDGF
                     LNEVEITRQMVTFKGYEINHHGIAAYCSHLPDDVDIDEMEREMKE"
     gene            4589..4867
                     /locus_tag="ACHOSM_RS23170"
                     /old_locus_tag="pgaptmp_004634"
     CDS             4589..4867
                     /locus_tag="ACHOSM_RS23170"
                     /old_locus_tag="pgaptmp_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560984.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDFWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
     gene            5000..5215
                     /locus_tag="ACHOSM_RS23175"
                     /old_locus_tag="pgaptmp_004635"
     CDS             5000..5215
                     /locus_tag="ACHOSM_RS23175"
                     /old_locus_tag="pgaptmp_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560985.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDFK"
     gene            5226..5462
                     /locus_tag="ACHOSM_RS23180"
                     /old_locus_tag="pgaptmp_004636"
     CDS             5226..5462
                     /locus_tag="ACHOSM_RS23180"
                     /old_locus_tag="pgaptmp_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HGU4560986.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RGAWAPSVAAAAEKWNRRAGDEANLSASQRSNQK"
     gene            5419..>5749
                     /locus_tag="ACHOSM_RS23185"
                     /old_locus_tag="pgaptmp_004637"
     CDS             5419..>5749
                     /locus_tag="ACHOSM_RS23185"
                     /old_locus_tag="pgaptmp_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HGU4560987.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSAPGIDGGVVMLGV
                     RTSKMRKA"
BASE COUNT         1652 a         1284 c         1471 g         1342 t
ORIGIN      
        1 ctgctttgaa tgctgccctt cttcagggct taatttttaa gagcctcacc ttcatggtgg
       61 ttagtacgtc ctgctgatgg ctaaatagta cgatttgtac tttattgggt caatacaaaa
      121 tgttctaaat ataattagtt ttttataacg ctttgtattt aatgggttta tattttggaa
      181 aaagaaaacc cgacgctaag gtcgggttat tgttgtgtgt tttagagttg tgaggctgtt
      241 aactaaatgt ctcttcaggc cactggctgg cgataacttt ccctactacg gaacagctat
      301 cattgcatgg aatcattgga tattgcgggt ttagtggctg taggaacacc tgaccgctat
      361 ccctgatcag tttcttgaag gtaaactcgt caccaccaag tctggctatg cagaaatcac
      421 ctggctcaac agcctgctca gggtcaacga gaattaacat cccgtcagga aagcttggct
      481 tggatcctgt tggcgcggtc atggaattac cttcaacttc aagccagaac gcacaatcac
      541 tggctttttt ggttgtgctg acccatctct ccgcatcacc tttggtaaag gttctaagct
      601 caggcgagaa catcccggcc tgaacatgag aaaaaacagg gtactcatat tgttttttaa
      661 cgggggcaga tgagtattcg ccaacaggtg aaaatgtacc gtcgtggttg aatgagacgt
      721 tatcaatacc aaggtattta aacaccacac caatctcgtc aagagatgga tgacgagatc
      781 cgcgcaacca gtgaccaatt ccaccctgcg tcataccaag ctcttcagct aacttctctt
      841 gagttatgcc gagctctttc attctggatc tagccagttc ataccatttc attttcatat
      901 ccttattatt acgctctgta ctaaaaccat ccatgcacaa gatgtatttt ttgtttgtat
      961 tctaaaagta catatcgtat tattgtttca tggttactat ggagggcata tgagcaacct
     1021 acgaaaatat cgagagtcac tgaatatctc tcaaacaaca cttgctaagg cagttggatg
     1081 cacacaggga gctatcggac attgggaatc tggtcgtcgc ttcccagacc ttaaaacatg
     1141 ccgcgctctt gttgcatgcc taaacaagtt aggcgcaaaa gtcagtcttg atgacgtgtt
     1201 ccctccggaa cacaaagccg cttaagacat tcccgctctt acacattcca gccctgaaaa
     1261 agggcatcaa attaaaccac acctatggtg tatgcattta tttgcataca ttcaatcaat
     1321 tgttatctaa ggaaatactt acatatggtt cgtgcaaaca aacgcaacga ggctctacga
     1381 atcgagagtg cgttgcttaa caaaatcgca atgcttggaa ctgagaagac agcggaagct
     1441 gtgggagttg ataagtcgca gatcagcagg tggaagaggg actggattcc aaagttctca
     1501 atgctgcttg ctgttcttga atggggtgtc gttgacgacg acatggctcg attggcgcga
     1561 caagttgctg cgattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     1621 atacaaatgg aattttaaca acatccaacg aggtaattat atgcgaaaca aaggctttaa
     1681 tccacctgat acacacaaag aagctaagcg tttgcgcttc cttcgttcca ttgatgaaag
     1741 aactcaaatc tcttttgtga aagttgccag aactgagctt ctgaaggctg aggcgagggc
     1801 gttgctcccg tctctaccaa agagggatat acgttcattc caaatgcatt tctggaaaag
     1861 ctgctcaaag aagacatatc cgtaagtcag tttaacgatg ttcttaaggt ctttcgtcaa
     1921 ggcaggtagt tatgagcaat acagcaaaaa tctacgattt cagcgccgca cacgagcgca
     1981 ggagcaacag gatggagaac cagaaaactg gttacattcc gttgtaccgg agcattctga
     2041 aacagtcatg ggcgaaagat gtttatcttc gcaccctgtg ggaaaacctt ctcctgaatg
     2101 ccgccagaaa gccatacaaa gcgaatttca aaggtcatga atggcatctg caacccggtc
     2161 aactggttgt gacagcagct gatttaggtc ttcagttatg cgacaggcat ggcaagccag
     2221 caagccgtga tcaggttgag cggatgcttc aggtttttgt gaaagagggg atgatcacca
     2281 ttgatggaga gaagcaaaaa ggtcgtgtga ttaccatcac aaattaccat gaatatgctc
     2341 aaaaaatgga cgattcaccc gcacatgaag ccgcacaaac aaccgcacat gatgccgcac
     2401 atgacgaagc cagcaatggc gcggctttca gagtgcatgc cgcacatgaa agcgcacatg
     2461 aagccgcaca aacaaccgca catcatgaac aagaaggtat taacaagaat ataaataata
     2521 cccccctacc ccccaatggg ggaggcgatg ggcaggttaa acctgaacgt cgaaaggcag
     2581 aacgaatcga ctacgaatcc ttcctgaacg cctacaacac cgaagtcggt gacagacttc
     2641 cacacgctgt tgcggtcaac gagaaacgca aacgccgcct gaagaaaatc atcccgcaac
     2701 tgaaaacgcc aaacgtggac ggtttcagag cgtatgtcag ggcgtttgtg catcaggcca
     2761 agccgtttta cttcggagac aacgacacgg gctggacggc agattttgat tacctgctga
     2821 gggaagattc gttaacggga gtacgggaag ggaagtttgc agacaggggg attgcatgaa
     2881 acaggatatc gaagcgagcg ttatcggtgg cttgctgatt ggtggattaa caccaaccgc
     2941 cagcgacgtt ctggcaacgc tggagccgga agcgttttca attccgctct accggaaagc
     3001 cttcgaggtt atccgcaagc aggcgagaaa cagaaaccta atcgacgcgc tgatggttgc
     3061 cgaggcgtgc ggagaggagc atttcacgtc aatcctgatg accagtaaga actgcccgag
     3121 tgccgcaaac ctgaagggat atgccggaat ggtcgcggat aactatcacc gccgtctggt
     3181 gctggaaatc atggatgaaa tgcgtgaacc aattcagaga ggaaccatcg atacatcgag
     3241 tcaggcgatg gacgagcttg taaagcgtct ttcagccatc agaaagcccc gtgacgaggt
     3301 taaacctgta cggttagggg aaatcatcac tgactacact gacacgcttg acaggcgtct
     3361 gaggaacgga gaagagtcag ataccctgaa gaccggaatc gaagaacttg acgccatcac
     3421 cggagggatg aacgcagaag acctggtgat aatcgctgct cgtcctggta tggggaaaac
     3481 cgagctggcg ctgaagattg ccgaaggcgt tgcaagccgc gttattcctg gttctgacgt
     3541 ccggcgcgga gtgttgattt tctcgatgga aatgagcgca ttgcagattg cagagcgaag
     3601 cattgccaac gccgggagga tgtcggttag cgtactgcga aatcctgcat cgatggatga
     3661 cgaaggctgg gcgcgcgttg ctaacggcat gagtcagctt gcagatttgg atgtatgggt
     3721 agtcgatgcc tcgcggttat cggtcgaaga aattcgctca atcgcagaac ggcacaaaca
     3781 ggaaaatcca aacctgtcac tcatcatggc ggattatctt ggcctgattg agaagccgaa
     3841 agcagatcgc aacgacctcg caattgctca catctcagga agccttaagg cgatggcgaa
     3901 agacctgaaa acgccagtta tctccctgag tcagctttca cgcgatgttg agaagcgacc
     3961 aaacaaacgc ccgacaaacg cagatttgcg tgattcagga agcattgaac aggacgcaga
     4021 ctcaatcatc atgctctatc gggaagcggt atatgacgag aacagtagcg ccgcgccatt
     4081 tgctgaaatc attgtgacga aaaaccgttt tggctcgctt ggtacggttt accagcggtt
     4141 ctgtaacgga cactttgttg catgtgacca ggatgaagcc agacagattt gcacaacatc
     4201 aaatgcacct gctgcgcgtg gcagacgata tgcacaaggg gctgacgtat gaataaaaaa
     4261 caactagcca ttctcgaaaa ggcatgggat gcacaaatat catgcgcttt gaaagaacag
     4321 gcactaccaa taatccagac caaatcgaaa atagccaggc agttatgcga tgacggattc
     4381 ctgaacgaag ttgagattac gcgccagatg gtaacgttca aagggtatga gataaatcat
     4441 catggtatag cggcgtattg ctcccatctt cctgatgacg ttgacattga tgaaatggaa
     4501 agggagatga aggaatgacc atctacatta ctgagctaat aacaggcctg ctggtaatcg
     4561 caggcctttt tatttggggg agagggaagt gaacgatagc taccgacagt ttgaaaactg
     4621 gtggtcaaaa gacaaaagcc agttcacggg agacgatgaa ttaaaagagt ttgcctgggt
     4681 gatatggcag gcatcgcgct ctgctattga actggatatc gactggcccg aatcgaatga
     4741 cgacttttgg aaagatggtg aagaaggtgc ttatgcgatg ggttatgagg atgggcgtga
     4801 caaaacggta attgcagtaa tgaaagccat cagggccgca ggaatcaaag aaaagaattt
     4861 cgattaagca aatatcactt caataaatcg cttttaaggc atcacaatcg ctctgtggtg
     4921 aggtaagcac gtgcaaggca tgccgataag cagcgagaat gaaaaatgcg tcagaatgcg
     4981 tttgaggagg ttttaagaaa tgagtacgat agctgagctt gtcagggcta attttcgtga
     5041 agagttggtg cgttggtatc ggtatcgttc atcgtccagt ttgccgcttg atgagttgta
     5101 tgagcattca cctgccgcac gacgctatcc gcgtgaccgt gttcttcgac ggttgttcaa
     5161 actcaacaat gagtttcagc gcaacagaat tatccggagt ctggatttta agtgaaggag
     5221 tgagcatgag cgacctatca ttaacccagc caaagctaaa agaatgtccg ttttgcggcg
     5281 gtaatgctcg tctgtgggtt gaggccggaa taaatattga tgtgtggggc tatgcagaat
     5341 gtgacctctg tgaagccagg ggggcatggg caccatcagt tgctgcggcg gctgaaaaat
     5401 ggaaccggag agcaggagat gaagcaaacc tttctgcttc gcaacgaagc aatcagaaat
     5461 aacgccatag acgccattct ctcactaccc atcgacgaca agtcacccca cgaagtccac
     5521 gttaaagaac ccaagcgcag caaagcgcag aatgaccgta tgtggccgat gctgaacgat
     5581 gtttcgcgtc aggtgctatg gcatggtcaa cggctggcgc cggaagactg gaaagacctg
     5641 ttcactgccc tgtggcttaa gaccaaaaaa ctggagcaaa gaagtgcgcc tggtatcgac
     5701 ggtggcgttg tcatgcttgg cgtgcgtacc agcaaaatgc gaaaggcca
//
LOCUS       DAZKHC020000189         5440 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZKHC020000189 DAZKHC020000000
VERSION     DAZKHC020000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.190"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            45..120
                     /locus_tag="ACHOSM_RS23190"
                     /old_locus_tag="pgaptmp_004638"
     tRNA            45..120
                     /locus_tag="ACHOSM_RS23190"
                     /old_locus_tag="pgaptmp_004638"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78..80,aa:Thr,seq:ggt)
     gene            160..275
                     /gene="rrf"
                     /locus_tag="ACHOSM_RS23195"
                     /old_locus_tag="pgaptmp_004639"
     rRNA            160..275
                     /gene="rrf"
                     /locus_tag="ACHOSM_RS23195"
                     /old_locus_tag="pgaptmp_004639"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(506..1264)
                     /gene="yhdZ"
                     /locus_tag="ACHOSM_RS23200"
                     /old_locus_tag="pgaptmp_004640"
     CDS             complement(506..1264)
                     /gene="yhdZ"
                     /locus_tag="ACHOSM_RS23200"
                     /old_locus_tag="pgaptmp_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGU4560988.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADQVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1272..2375)
                     /gene="yhdY"
                     /locus_tag="ACHOSM_RS23205"
                     /old_locus_tag="pgaptmp_004641"
     CDS             complement(1272..2375)
                     /gene="yhdY"
                     /locus_tag="ACHOSM_RS23205"
                     /old_locus_tag="pgaptmp_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU4560989.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MCELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHEQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIIWWLMYGGFFALERVETRQWDGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2385..3566)
                     /gene="yhdX"
                     /locus_tag="ACHOSM_RS23210"
                     /old_locus_tag="pgaptmp_004642"
     CDS             complement(2385..3566)
                     /gene="yhdX"
                     /locus_tag="ACHOSM_RS23210"
                     /old_locus_tag="pgaptmp_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU4560990.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRKIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FALAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3634..4659)
                     /locus_tag="ACHOSM_RS23215"
                     /old_locus_tag="pgaptmp_004643"
     CDS             complement(3634..4659)
                     /locus_tag="ACHOSM_RS23215"
                     /old_locus_tag="pgaptmp_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4560991.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5090..5311)
                     /locus_tag="ACHOSM_RS23220"
                     /old_locus_tag="pgaptmp_004644"
     CDS             complement(5090..5311)
                     /locus_tag="ACHOSM_RS23220"
                     /old_locus_tag="pgaptmp_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560992.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
BASE COUNT         1503 a         1388 c         1303 g         1246 t
ORIGIN      
        1 tccccatgcg agagtaggga actgccaggc atcaaattta gcgtgctgat atggctcagt
       61 tggtagagcg cacccttggt aagggtgagg tccccagttc gactctgggt atcagcacca
      121 ctttttaggt taaagttcgg cagattagaa aagaatttgt ctggcggcag tagcgcggtg
      181 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
      241 ggggctcccc atgcgagagt agggaactgc cagacatcaa ataaaacata aggctcagtc
      301 ggaagactgg gccttttgtt ttatctgttg tttgtcggtg aacactctcc caaccaagac
      361 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
      421 acgcccgcca taaactgcca gacatcaaat caagcgaaag gccatccgaa aggatggcct
      481 ttttgctttt tgaactaaca ttcaattaat ggattacctg cgataaaaat gccctcgtac
      541 gctctgattt aggatgcgca aaaaattcat caggtgcagc ttgttccact atctccccac
      601 gatccataaa aattacctgg tcagcgacgg tacgtgcaaa ccccatctca tgtgttacac
      661 acaacattgt catacccgac tgcgccagcc caatcatcgt atccagcacc tctttcacca
      721 tctccggatc gagcgccgac gttggttcat caaacaacat aattttcggc ttcatacaca
      781 gcgaacgcgc aatggcaacg cgttgctgct gaccacctga aatctgtccg ggaaacttat
      841 gcgcatgttc ggcaattctc acccgctcaa ggtaatgcat cgccagagct tcagcctctt
      901 tcttaggcat cttgcgtacc caaatcggtg ccagggtaca gttctgtaaa acggtcagat
      961 gagggaagag attgaaatgc tgaaatacca ttcccacttc ctgcctgacg cgctcaatat
     1021 tgcggatatc ttcattaagt tcgatgccat ctacgacaat ccgtccctgt tgatgttctt
     1081 ccagatgatt aatacaacga atggttgtcg atttaccgga accagaaggg ccacacagaa
     1141 cgatccgttc tcccggttgc acggttaaat ttatattttt caaaacatgg aattgtccat
     1201 accatttatg gacgttttcc agcgtaatca tcgcgttagc aggttgcagt aaaatttggc
     1261 tcatagtgtc ctcaatgcgg tgtacgcccg gtgttaaaac gtttttccag atgttggcta
     1321 taacgtgaca tgctgaaaca gaagatccag tagatcagcg cggcgaaaac atacccttcc
     1381 gtcgacatac ccaaccaggc gggatcaacg gttgcctgct gaacgctact gaaaagatcg
     1441 aacaagccga tgatgatcac cagactggta tctttgaaga gtgcgatgat ggtatttacc
     1501 agcccaggaa ttaccagctt caacgcctgt ggcagaataa ccagcccctg agttttccag
     1561 taacccaacg ccagcgactc tgccgcttca tattgccctt taggtagggc ctgtaatccg
     1621 cctcgcacga cttccgcaac atatgctgac tgaaacagga tcacgccaac cagcgcgcgg
     1681 atcagtttgt cgatactggt gccttctgcc ataaacaacg gcagcatgac cgaagacata
     1741 aacaatacgg taatcagcgg tacgccgcgc cagaactcga taaaaatgac cgataagata
     1801 cgcacaatcg gcatatggga gcggcgacct aatgccagta agatccccca cggtagcgcc
     1861 cccgcaatcc caactgatgc aataattaaa gttagcgtca gcccgtccca ttgccgggtt
     1921 tcaacccgct caagagcaaa aaaaccgcca tacatcagcc accagataat cagtgggtaa
     1981 atcaccgccc aggccgcaat atagcgaccg cgatgcggga gtattttcca gaacattggt
     2041 gcgatagaca ctaggccaat cagtaatgcc aggttaattc gccagcgttg ctcgtgtggg
     2101 taaagcccat acataaactg accaaatcgt tcgtggatga agacccaaca ggcaccggct
     2161 tttgtgcagt cggcacgcgt tgagccaacc cagttagcct gcaaaaatgc ccagttcagc
     2221 aacggtggga tcagttcaca catcaaccat atacagccaa tagtcagtag gctattgctc
     2281 cagctggaga acagattttt tcgcacccac accatagcgc ggcttgagtt atggctcgcc
     2341 gggcgcgggg gatgagacag caatactttt gtcatcatga ctccttagcg ttccacgatc
     2401 gcgatgcggc ggttatagat attcatcagc agcgagatag tcaggctgat aatcagatag
     2461 accgacatgg tcatggctat cgtctcgatg gcttgccccg tctggttcag cacggtgcca
     2521 gcaaacagcg aaaccatatc gggatagcca atagcggcgg caagagagga gtttttgacg
     2581 atgttgagat actggctggt taacggtgga ataatcaccc gcagtgcctg gggaataatg
     2641 acctggcgta gcgtaaccgg gttgggtaat cccagtgacc gagcagcttc atgttgacca
     2701 taaggcactg cctggatccc agcgcggata atctcggcga taaatgcaga ggtataaacc
     2761 gaaagtgcca gcgttaaggc tgccagttca ggaattaaaa ccatcccgcc gcggaagtta
     2821 aagccttgta gggctgggac atcccagtgt agtgctgcgc caaaaagcca ttgcgccagt
     2881 aaaggcaaac caatgatcaa cactgcggcg atgggccagg tgcggcgcag ttgtccggtc
     2941 tttatctggt atgttttatt aaagcggaat agcccaacag aaaggactat agccataaca
     3001 acagccagga taaacgcaat aaatccgtct cccagctgcg gtgacggaat ataaagccca
     3061 cgattgctca aaaaggcgag agcaaacgcg ctcacagcct ggcgtggtcc gggcaaattg
     3121 cgcaacacgg caaagtacca gaagaagatt tgcagcagcg ggggaatctt acggaagatc
     3181 tcaatataaa ttgtggaaag ctttcgtagc agccagttat ccgaaagtct cgccagaccg
     3241 ataaagaagc ccaaaacaga agcgaacaca atacacaatg cagaaaccag tagcgtattg
     3301 agtaagccga caataaaaac gcgtccgtag gtgtcgccct gctggtaatc gatcaaatgc
     3361 tggacaatac cgaaaccagc gccgcgatcc agaaaggcaa aacctgaagt aatgccacga
     3421 ttgctcagat tcgttacagt gttgtgaaat aaccaaccaa caatgccgac aacagcaaca
     3481 acggcaagga tctggaataa ccaggcgcga accgtagggt tggcaaaaga gagtgagcct
     3541 ttaacggttg agcggcgatg agacatagaa acctcggaaa gcataacgct gaagagagcg
     3601 ccgccgtagc gacgcccgtt acctcacacc gacttaacgc acgggcggcg cgtactgaat
     3661 accgccgtta ttccagagat tattttgccc acgtttaatt ttcagcgggc tttctgaacc
     3721 tacgttacgc tcgaaaattt ccgagtagtt acccacctgt ttgatgatgt tataggccca
     3781 tttattatcc agcttcagat ccttgccgta atcgccttct ttacccagca gatgtgccat
     3841 atcaggcgtt gctggattag ccgctttttc atcgacgttc tgggaattga tgcccatctc
     3901 ttcagcattc agcatggcga aaagcgtcca gcgtacaatc gagaaccatt catcatcgcc
     3961 acgacgaact accggcccaa gcggttcttt agagataacc tccggtaaga caatccattc
     4021 agctgggttg cttaatttga tgcgcagggc atacagttgc gactgatccg aggccagcgt
     4081 atcgcagcga ccagattcca gtgccttcgc tgattcgtca gagcgatcga aagtcaccgg
     4141 tgtgtacttc atattgtttg ccttgaaata gtcggcaacg ttgagttcgg tatcagtacc
     4201 cgcctgaata cagacggtag cgccatccag ttctttcgcg ctttttagcc ccgctttatc
     4261 gtgcgtcagg aagccaatgc cgtcgtaata agtgacgcca gtaaatgcca ttcccatccc
     4321 ggcgtcgcga gatgaagtcc aggtcgtatt acgggagagc aaatccactt cccctgactg
     4381 taaagccgtg aagcgttctt ttgctgtgag tggggtatat ttcactttcg tgtcgtcacc
     4441 aaatacagca gcggcaacac cacgacaaac atcaacatca atacctgaaa acttaccgtc
     4501 tgcatcggca taagagaacc caggtaatcc atcactgatc ccgcattgca caaaaccttt
     4561 tttctgaacg gcatcaagcg tcgcgccagc atgcgcctga tttgcaacgg caagcagcac
     4621 gctggcggca gccagtgtgg ctatcatcat ctttttcata atgcttcctg tgaggcgaaa
     4681 attatcgtta taggacgttt gggaaacgtc ttttacctgt ctgtggccat cctcttataa
     4741 gcaaaagtag tgccagcttt tgcgcccgat ttcgcgtcgc aataagctga gtgtagacag
     4801 gaaataataa aaacagcgcc ccgcaatggt gcgaaaatga aacatgcgcc acattttggc
     4861 gcaaaatatt tacgcgtgtt ttactaagca gaataatgat tgttagcgtg agtatatttc
     4921 tggtgggaag tatgaaagtt aaattgatgc gttatttcga aagcagcgaa ataacgcgat
     4981 ataacatgct aaatcagcag agaattagcc agcagaatat agcaatattt tatcttatgt
     5041 aaagatactg tatcgttaaa cagagcgcga aactccgcgc cctgttcagt tatttcgaca
     5101 gagcgatgat ccccatcgtc aggccagcaa cagccgctgc cccgccggca atagccccag
     5161 ccgtttccca gttatcctga gtcgtgcctt tcatacaggt attggtgtga accagacgcc
     5221 cctgatcgtc ataaaccgga acgcagggcg aatcgtgagc acagcccgcc agcaatgcgg
     5281 tgagcaatgc cacaggaatt aatcttttca tggtgttacc tcagtaataa acgctaacgc
     5341 ttaataagtt aactctctta ttaacgagct gaatgccatt ttatcaccgg aggttataaa
     5401 tattgcggaa tgaataatga caaattatgt gaattgtaaa
//
LOCUS       DAZKHC020000190         5423 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZKHC020000190 DAZKHC020000000
VERSION     DAZKHC020000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.191"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..354
                     /locus_tag="ACHOSM_RS23225"
                     /old_locus_tag="pgaptmp_004645"
     CDS             <1..354
                     /locus_tag="ACHOSM_RS23225"
                     /old_locus_tag="pgaptmp_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HGU4560993.1"
                     /translation="KSNIFGTSFTNTMIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVF
                     KTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELNKFMHDYDKTSSKMRKYLQS
                     INECMLLIKNIVHQD"
     gene            588..1940
                     /locus_tag="ACHOSM_RS23230"
                     /old_locus_tag="pgaptmp_004646"
                     /pseudo
     CDS             588..1940
                     /locus_tag="ACHOSM_RS23230"
                     /old_locus_tag="pgaptmp_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1953..2912
                     /locus_tag="ACHOSM_RS23235"
                     /old_locus_tag="pgaptmp_004647"
     CDS             1953..2912
                     /locus_tag="ACHOSM_RS23235"
                     /old_locus_tag="pgaptmp_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGU4560994.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCKGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFESSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(2950..3849)
                     /gene="nhaR"
                     /locus_tag="ACHOSM_RS23240"
                     /old_locus_tag="pgaptmp_004648"
     CDS             complement(2950..3849)
                     /gene="nhaR"
                     /locus_tag="ACHOSM_RS23240"
                     /old_locus_tag="pgaptmp_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HGU4560995.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVDGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(3915..5081)
                     /gene="nhaA"
                     /locus_tag="ACHOSM_RS23245"
                     /old_locus_tag="pgaptmp_004649"
     CDS             complement(3915..5081)
                     /gene="nhaA"
                     /locus_tag="ACHOSM_RS23245"
                     /old_locus_tag="pgaptmp_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HGU4560996.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1707 a         1188 c         1097 g         1431 t
ORIGIN      
        1 aaaagcaaca tatttggaac atcgttcact aataccatga taaaaaattt gcgctgtgaa
       61 gatattatgc ctggccgatg gaccactcaa cttgtcaata agcacttagg ttacagatac
      121 acaggagtat tcaaaaccct ggcaagtatt gatgacaaac cgtcccgttt cgaaattttg
      181 ataccattgg tacaaacttt agttcgcgac aatgtaaagc taaataatga tgtgtataaa
      241 gaacttaata agtttatgca cgattacgac aaaacatcat caaaaatgag aaaatacctt
      301 cagagcatca acgagtgcat gctccttata aaaaatatag ttcatcagga ttaaaacact
      361 caaagaaaga ggtaatttta gaatgaaatt atctgtgatg attattaata cactgcagag
      421 aaataaaaaa caatctgaag catgctctga aacatcctac tctgtcactc taatgatggc
      481 accttgctct tcttataaag aagggaacaa ttaagaatat taaattttca aactttcatc
      541 aacttcacat caagaaatta aaaaatttaa acattaatct aaataataac aacctgatca
      601 cataatcggg tacgatttta tccgataact ataatgctat aactctggga aatggttgga
      661 atacaccgct gggtgccatc tctttttacg ctgcccgatc gagtagtaag ttaaataatg
      721 ataccagaca cgaaggtacg agctatcagg tcgcctataa taaatatcta gtacaaacag
      781 ccactcgatt tagcgtggcc gcctggcgtt atgcttcgca ggattacagg acattcagcg
      841 accatcttta cgaaaatgat aaacactatc atcagagtga ttatgatgat ttttatgata
      901 ttggcagaaa aaatagcctt tctgctaata ttatgcaacc tttatccaat aatctaggaa
      961 atgtatcatt aagtgcgctt tggcggaatt actgggggcg aagtggaaat gctaaagatt
     1021 accaattcag ttactccaat aactggcaac acattagtta tactttctct gccagccaat
     1081 cttatgatga aaataataaa gaagaggagc gttttaatct gtttatctcc attcctttct
     1141 actgggggga tgatattgcc aaaacacgtc accaaattaa cttatcgaat tcgacctcat
     1201 tttccaaaga tggctattcc tccaacaata ctggaattac tggcatagcc ggtgaacatg
     1261 atcagttaaa ttatggtata tatgttaatc agcaacaaca aaataatgat acctcgcttg
     1321 gtacgaattt aagctggaga actcccatcg ccataataga tggcagctat agtcattcta
     1381 aaaacgcctg gcaaagtggt ggaagtatta gtagtggatt agttgtctgg cccggtggta
     1441 ttaatatcac taaccagtta tccgatacat ttgcaattct ggatgcgcct gggttagaag
     1501 gtgcacatat taatggacaa aaatacaatc gaacaaacag caaaggccag gttgtttacg
     1561 acctgataat acctcatcgt gaaaaccatc tggtacttga tatagcaaac agtgaaagtg
     1621 aaacagaatt gcagggcaat cgtcaaatta ttgcacctta ccgtggagca gtttcttatg
     1681 tgcagtttac aactgaccaa cgtaagccct ggtatataca ggcactgcgt cccgatggtt
     1741 cgccattaac ctttggctat gacgtactgg atctccagga aaataatatt ggagtcgttg
     1801 gccagggtag tcgccttttt attcgcgtag atgaaattcc cactggaata aaagttgctc
     1861 tcaatgatga acagaactta ttctgtacta ttacttttca acacgttatc gatgaaaaca
     1921 aaacatatat atgccagtaa aaggagttta ctatgaagtg gcttttatta atcaccttga
     1981 gtctatattc ttttattgtg caaagtgctc cctgtgcatt gaccaatgtc ggggaacaac
     2041 gtggaacata tatactcaag ccactgagta tgaaaggtaa tctcaccgcc gaaaaaattg
     2101 aaaacaccgg aaatatcatt gagtgtgcag acttacaaga gacgaccaac gccattaaag
     2161 ttcatttttt aacacagaac gctttaaggt ttaaagtcgg aagtcgtcat tatattatta
     2221 atttcccctt tgaatctgac ggtaaaaaaa tagagcttcc aggaaggtca tcttataatt
     2281 tagaagaaat gctatctgcg ataaatttca ccattccata ctctatcgca tctgtatcag
     2341 atagttccaa caccgcagat atttttccag gaactccttt tcctttgttg ttcgaaattg
     2401 aagtgagttc atgtaaaggg aataatacta attgtaatag cgtccgcttt aattataatt
     2461 taaatactat tttacaggta aatttaatga cctgtggttt tgaagatcaa tccatcgaca
     2521 ctggacgatt tgtactgtct cgtatcaata atgaaaatta tcagtttcat catataagat
     2581 ttgactgcat ccagggcgag caaggtgaat tagtttttga atccagtgat gttgaatatt
     2641 actttgaacc agtgacgata caggagtcag gaaccagcat attaaaaaat gagttagaaa
     2701 acagtgagga cggtgcgggt caagtaggct ttcaactctc caatgatggt acgaatgaaa
     2761 tccagtatgg taaaagcaat tactactctt ttcagcatcc ccatgaagga agtaatcaga
     2821 ttcctctatt tattcgcccg cgtacatatg gaaataatgt ctcaagcggg caaataatga
     2881 gccgagtcaa aatcgtggtt atgtacaact aatctatctc ccccacctta actttggggg
     2941 aggtgccgat taacgcgccg ctggactaaa aagcgcagaa taatccgtat tgcagattcg
     3001 ctgtaccgcc gggtgctgaa tcatccgctc agcaaaaata gcatggtact cttccatcac
     3061 attctcgacg cgaccaattt ctacgacagt tttatcggca taaaagtcat atgcataaag
     3121 cgttggggca acgaagattg cattgtgcat cgcaccaaaa gctttcatca acgcggcatc
     3181 atcaaactcg ccgaggattt ctacgtttaa tccctgggag ttaaaccagt taagcaattt
     3241 gcgccctaac attgaacgtc gcccaggaat caaaagtcgc cgttcttcca gacaagccgg
     3301 gaacggtttt tctggtggtg gatttgtaca ccagaaactc acgccacatt cgccaattct
     3361 cacggagaac aggccttcct gctgcgtaga gtctatcgga cagtcagaaa tgatcatatc
     3421 cagtttatgc tgacttaatt gctccagtag catttcgtgg gtggattcga agcagcgaag
     3481 atgaatgggc tcgccgtcta ccactgcggc gttaagtacg ctactgacca ggcgtttgga
     3541 aagtgcatca gccacgccaa cgtcaaacaa taaattggat tctttgcgat agttcacaat
     3601 atccagcatt tcctggctta aggtgaacat tttatcggca tagcgataga ccagttctcc
     3661 cagctcgctg ggttcgagac cacgtccctt gcgtttaaat aatttgcctt gcaggcgctc
     3721 ttccagcgct cgaatctgtc cggtaatggt ttgtggagtt aaataaagcg cctccgctgc
     3781 gccaaccacg gaaccttctt tatagacatg ccagaagtaa tacaagtggt tgtaattgat
     3841 atgagacatg ctcatttctc tccctgataa caatgaaaag ggagccgttt atggctcccc
     3901 ggtaaaccgt cctgtcaaac tgatggacgc aaacgaacgc gtaaccagct gtatccaatt
     3961 accgccgaag agatagaacc gaccaagata ccgagtttcg cccagttaat cagttctgga
     4021 tctacgctac caaaggccag gctggcaata aagatagaca tagtaaaacc gataccgcac
     4081 aggatcccca ccgccataat ttgctgataa gtcgttccct caggcagatg cgccagtttc
     4141 aaacgcagcg ccaaccagca gaacagacta atccccagcg gtttgccaat cagcaagcca
     4201 gcgatgatcc ccaatggcag aatggaggtc aagccatcca gcgtgacgcc ttgcagtgaa
     4261 acgccagcat tagcaaatgc aaacagcggt aaaatcagat acgccaccca cgggtgcaat
     4321 acatgctcca gtcgcttcgc tggagaacgc ccatgcttct ctttcaaagg aataaagaag
     4381 ccgacaatta cccccgccag ggttgcgtga acccccgatt tcaacaccgc agtccacaac
     4441 accacgccaa caagaatata gacgcccgtg cggcgtacac cacacagatt caataccgcg
     4501 agtaccgcaa ttgctacagc cgcgacgcca agagaggcca tcgataagtc attagtgtag
     4561 aacaatgcga tgataatgat ggccccaaga tcgtcgataa tagccagagc catcaaaaag
     4621 atcttcagtg ctaacggaac acgacttccc aacagcgcca gcacaccaag tgcaaaggca
     4681 atgtcagtag ccgccgggat cgcccaccct tcgcgggtaa tcggatcggc atagttaaag
     4741 gccagataga gtaatgccgg aacaatcatc ccaccaatag cggcgataac cggaaatgcg
     4801 gcctggcgta agctggctag cgatccttgc atcagttcac gtttaacttc cagaccgact
     4861 aacaggaaaa ataccgccat cagcgcgtca tttatccata acagcatgtt tttgttgatt
     4921 tcgagtgaac caacccggag ctgaaccggc gtctccagaa agtcgtgata ccatccactg
     4981 gttgcgccgc tgttggccat catcatcgcc aggatagcgg caatgataag aataatgcct
     5041 cccgaggcat cactgctaaa gaatcgatgc agatgtttca ctttttattt ctctttcagg
     5101 tgaatagatc gtttttacta ttttaccccg cacgaatcat cgttaaaatc agattatagt
     5161 gtaggaataa ctcgtttttt acgagcaatt aaaggatttg cgccatcagc ggtgagatga
     5221 gtttttgacg gctgtaagcg ctaacgggtg accctaagcc ctgataatca tagcttaagg
     5281 tcaggatttc tgagggatta attaggaaaa gtaataatta atttcttgtc agtcggcgtt
     5341 atttgttagg acaatatttc aaaaataatc cagcttgtcg tcaataattg cgataaatag
     5401 ataaattaaa gttaaacaat taa
//
LOCUS       DAZKHC020000191         5340 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZKHC020000191 DAZKHC020000000
VERSION     DAZKHC020000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.192"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..264)
                     /locus_tag="ACHOSM_RS23250"
                     /old_locus_tag="pgaptmp_004650"
     CDS             complement(<1..264)
                     /locus_tag="ACHOSM_RS23250"
                     /old_locus_tag="pgaptmp_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4560997.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYDASHLRPERRRRDILLEEVLRQAGIPLLRSHDAR"
     gene            complement(302..511)
                     /gene="hha"
                     /locus_tag="ACHOSM_RS23255"
                     /old_locus_tag="pgaptmp_004651"
     CDS             complement(302..511)
                     /gene="hha"
                     /locus_tag="ACHOSM_RS23255"
                     /old_locus_tag="pgaptmp_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HGU4560998.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(557..1018)
                     /locus_tag="ACHOSM_RS23260"
                     /old_locus_tag="pgaptmp_004652"
     CDS             complement(557..1018)
                     /locus_tag="ACHOSM_RS23260"
                     /old_locus_tag="pgaptmp_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HGU4560999.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            complement(1263..1475)
                     /locus_tag="ACHOSM_RS23265"
                     /old_locus_tag="pgaptmp_004653"
     CDS             complement(1263..1475)
                     /locus_tag="ACHOSM_RS23265"
                     /old_locus_tag="pgaptmp_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HGU4561000.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            complement(1608..2168)
                     /locus_tag="ACHOSM_RS23270"
                     /old_locus_tag="pgaptmp_004654"
     CDS             complement(1608..2168)
                     /locus_tag="ACHOSM_RS23270"
                     /old_locus_tag="pgaptmp_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HGU4561001.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            complement(2271..3131)
                     /locus_tag="ACHOSM_RS23275"
                     /old_locus_tag="pgaptmp_004655"
     CDS             complement(2271..3131)
                     /locus_tag="ACHOSM_RS23275"
                     /old_locus_tag="pgaptmp_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4561002.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            complement(3190..3936)
                     /gene="traX"
                     /locus_tag="ACHOSM_RS23280"
                     /old_locus_tag="pgaptmp_004656"
     CDS             complement(3190..3936)
                     /gene="traX"
                     /locus_tag="ACHOSM_RS23280"
                     /old_locus_tag="pgaptmp_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HGU4561003.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGITG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            complement(3956..>5340)
                     /locus_tag="ACHOSM_RS23285"
                     /old_locus_tag="pgaptmp_004657"
     CDS             complement(3956..>5340)
                     /locus_tag="ACHOSM_RS23285"
                     /old_locus_tag="pgaptmp_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HGU4561004.1"
                     /translation="IAGISRDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRVG
                     TGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSGGQQTRVIRPAQERAEQHIDLAY
                     AITAHGAQGASETFAIALEGTEGSRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDA
                     INNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPAR
                     FIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFV
                     ALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPD
                     NSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGRTEQAV
                     REIAGQERERAVTSEREAALPESVLREPQREREAVREVVRENLLQERLQQMERDMVRD
                     LQKEKTLGGD"
BASE COUNT         1194 a         1607 c         1183 g         1356 t
ORIGIN      
        1 tctggcatcg tggcttctga gcaacggaat accagcctgc ctcagaacct cttccagaag
       61 aatatcccgg cgtctgcgtt caggtcgtaa atggctggca tcatattcac cgccggacag
      121 tattcgctct gcatcaccgg tccgtatacc tgccgcatgc aattgcatca gcaaaggaga
      181 atgctgattt acacttctga ggataatccg gataagtaac gctgcaataa tcagaacaat
      241 gagaataaaa ataatcagct tcattgttta tctgctcctg gtaattaatt atgctgaatt
      301 attaccttac atatttccat atttcttttg gtatcctgtc atataacttt ccggtgatta
      361 attctgctaa ccggtggtct gctgctgaat taaatgattc acgttcactg gtcgataaac
      421 tgtctctgtt tttatgaata attttttcca gcgtattgac tgatgaacat cgccgtaatt
      481 gatacagcca ttcctgtttt gttttttcca tttttattcc cgctgagaac agatgaatga
      541 taagattgcc atcgctttat ttacggtgtc tccatatcca ggggggaacc ggatcagcat
      601 ctgaccagag cccacgtttt tgttgtctgg cttcgttctg aaggacaggc agcacgggat
      661 ctgtgttgta ctgctcatat acccaggcgg ccccggcccg gaccataaac tgattaacat
      721 tcattccgtc tggtgcataa acctgcccga gaatacgacc ataacgatct cgctgaaaat
      781 atgtgacggt aaccgttttt cctgcaacca gtgatttcat catgtctgtc gaccaccggc
      841 cataatcctg tttcttttcc ggcgcatcaa tatttaccag cctgatccgc accgccttcc
      901 gtgagtccat gacctcgatg gtatcaccgt ccagtacacg aacaacccgt ccatgaatat
      961 ctgcacacaa taccggccat gaaaagataa ataataccag tggaatatat ttcctcatgt
     1021 tattctccgt atcagataaa tagcacctgt atttcaggca tcctgaatat tgcagcacac
     1081 actgaataac accttatttt gtgaaattaa tcacaaaata taaaatcacg acttaatacg
     1141 gaaaaaactc tgtgctactc agaactggcc ggtcatactg cccgaccgga tattaaaaaa
     1201 agcaaatctc atgcattatt cagccttctg aatccctgga gtaatgaagg ctgctttttt
     1261 cgttaccccc ttcctcggca gcgccggtgg cagtgttcag ccctttttcg ggcaaactgc
     1321 tgccagtcag tccggggtgc aacaccggca gcccgtcgcc atgcttctgc cgcttccatc
     1381 cagcgtccac tgacttccag aataacggcc cggcttccgg cacgctggta acgccagacc
     1441 tgaccgttcc gtgaactgtt tctgaatcca ttcatcttta tcactcctct ttaacccgtc
     1501 agtattatgg tgttaaataa tctactgaat tggcttcctt tcgggtacgg aagttatttc
     1561 cttttaagcc ttatttccct gcctcctctc tctgagggag gcttttttta tttctcatca
     1621 agcacggcct gaagttctgc ctttatccgg ttctggcggc ggattttatc cagacgcgct
     1681 gccgcatacg cttcctcctc ctgagaaata tgctccgtca catacccttc cgtgtcatac
     1741 cggcaggcac cggctttcat ggcacacaga tagctttctg aacgggtgat ggccttcagc
     1801 gccctgcgca gctttttatg cgagagcggg atattccgct gcgccacgtc ttccagtaac
     1861 acgtcccgga taccgcaggc cagaagccgg ggcgtgtcac cgtcaaataa tcccggccac
     1921 cagggtttca gggtgttcac ggcctcatcc agcgtgggca ggttcagata aatggacagc
     1981 gcctgtctgg cctgcgcttt ttttgccgcc agctccgctt cccgggctgc tttctcggcc
     2041 agcttctgct ttttcacctt ccattttggt ggcgtggtga cattgatgat ggttttccgg
     2101 ctgcggacag gcgtctctcc ttccgttttc cgcttcagtg tcagtaccgg tcgtttttgc
     2161 tctgccatgc ttactctgac cttcgtgaaa atatgccctg caggccgttg aaacggctca
     2221 tcgtaacagg aagttaattt caacgacctt taacgtataa atactgacaa ttaaagatat
     2281 tgtttaaacc attgtgtctg cacagctact acacgttcaa aaaacgcgcc ctcataaata
     2341 tcataatggc atgcatcagc ttcctcgtat aattccttag taccagaagc aacagcatca
     2401 tataatgctc ttccctgttc cggtggatta acgctatcct ggccggcaat aacaaccaaa
     2461 actgggcact ggacgctggc tgcactctct gccggtttat attgcaacgt ttccatgacc
     2521 gtaaggaaag gtatttttat atccatttcg ggatgccggg ctttgatttt ctcgaagaat
     2581 accttcgatt catcatcact tagtacccgt gtaaccccca caaacatctc tttcccggtg
     2641 tttttcttct tctctgccat tttattcagc gtggatagaa aagacgctct ctccgattca
     2701 ttcatctctc cggtaaccag aacatcaccg tcagcaaaag ccagctggct gacaatacat
     2761 tttacgcgtt ggtcctgcgc agctgcactg aagacatgcc cccctcccag cgatgttccc
     2821 cataacccaa tacgctgatt atcaatacat tcctgctttt ctgcccaatt gataactgaa
     2881 ataatatctt cggtctgcat tgcaggaacc agacgccctc gttctccatc actctcaccg
     2941 aatcctcgat aatcaaaagt aatcgttgcg aatcctgctt ctgtgaaggc attcgcaaag
     3001 caagggagca gaacattcct gataccgcaa aacccatggc ataagataat caaaggatgt
     3061 tttatatttc cttcaggaac acgaaatgtc agggcaatac cttcagagag tttgtgatcc
     3121 gtaattttca taaatatcct gtgttatgtt gtttattaat aagcagaatc agcgtcagta
     3181 tcataccagt cacaacgcca gaatgcccag cacagccaga tgacagccat agaacgtcgg
     3241 gaaaaaatct ccgggccaga aacggggtaa tgatttccct gcacaggaca ccagaccaac
     3301 ggtcagcacc gtcatcacaa gaccagctac agccgccgca tcactggagg caaggttaag
     3361 tgccggaatc acggccagca ggcatgcaac cagcaccaga cgttccgttc tgtcttccgc
     3421 ccggtacagc cggtgactga ctgccagcat cagcagcccg gtaatgccgt aactggtgcc
     3481 ggacaaaggc ccccacagtg ccatcaggag aatggccgct gctgtacgcc acccactgcg
     3541 cgtttcacac cacgtcagca cctgtgccgc cactgcaaag gcaaacagga tattcccctc
     3601 ataccacgga aagcctgcca gataatacgc gaactgggca ataattcccc atccccacag
     3661 ccggttaatg gccgactgcc tgatatgcgc atggcgggac agattcagcc cccacaccag
     3721 agcaaacaac ggaaaggctc cacgcccggc cagaaacatc cacgtctggt ccagatgcag
     3781 aatgcggttt gcgtggtcca gtaccatcag caccagtgcc accgttttga tgatatcccg
     3841 ctgtccgggt gaccagacca gcaacgactg caaccaggta tcggtccggg cagccagtga
     3901 atcgttacgt gtcgtgttcg tgttatccgt tgtcatcagc gtttatcttc ctgtatcagt
     3961 ctccgcccag ggttttctct ttctgcaggt cacgaaccat atcccgctcc atctgctgca
     4021 gtcgctcctg cagcaggttt tcccggacaa cctcacggac cgcctcccgc tcccgttgtg
     4081 gttcacgcag tacactctcc ggcagcgcgg cttcccgttc agaagtgaca gcccgttcac
     4141 gctcctgccc ggcaatctcc ctgacagcct gctctgttct gccgtccggc aggtcaggtt
     4201 tgttctctgc cattttacgg acaatttcat ctgcgcggcg ttccgtttca cggcggatgg
     4261 cctcttcagc ctgccgctgt gccagtacct ctgccgtgac cggctcgcca tttccggctc
     4321 ccggctggac gctgttgtcc gggatatccc cccacacgcg accaccggtg atggcaccgg
     4381 gattccacgg acgaccttca ccggcgattc tcaccaccac accgctgtca ggattatcac
     4441 gggcaatccg gacaccatcc tgcatattat cagccagcag gctctctccg ttacggctgc
     4501 cctgcagggc cacgaactgc gcatccccgc tgcctttcac ccgtccttca ccactgaatc
     4561 cccgcagccc gtttccgtca tccgtggtca gcgggttcag ccagataccg gcagacttgc
     4621 cgttacggtc aaacgccggc agtgccacat acggctgtgg atatttacgt cccggagcaa
     4681 taaaccgtgc aggactgtct cccccggcaa gccccgcctg acgaagaacg gcacgccctg
     4741 ccgccacgtc ccgcagctcc cgcgccgtac tgaacagccg ctctgcattc atgacctccc
     4801 ggtccggttt cggttcaaac acatcgtggg ctgttccttt ctgtacggca ttgttaatgg
     4861 catccgtcca gccctgacgg ttatcggtgt acacctgcac atgctgcttc atacgcgaca
     4921 gggccacgta ggctgactca aagccggcca tcagtttccg gctgccttcc gtaccttcaa
     4981 gcgcgatggc aaaggtttca cttgccccct gcgcaccgtg agcggtgatg gcataggcca
     5041 ggtcaatatg ttgctctgcc cgctcctggg cggggcgaat cacccgggtc tgctgtccgc
     5101 ccgacagcgt gacactgtca ccggaaaccg ctgtcaccgt ccagacgctg ttggccacat
     5161 aaccacgctc ccggtcactc ttcgtgaagc gcatccggtc accggtcccc accctgatgg
     5221 tgtccggggt gtacagtgtg acgccttcag ccaccgcctc ccggggcgaa atcagccgcg
     5281 tgttaccttc cgcatcctgc agggttatca gcccgtcatc cctgctgata ccggcaatcc
//
LOCUS       DAZKHC020000192         5339 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZKHC020000192 DAZKHC020000000
VERSION     DAZKHC020000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.193"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..288
                     /locus_tag="ACHOSM_RS23290"
                     /old_locus_tag="pgaptmp_004658"
     CDS             <1..288
                     /locus_tag="ACHOSM_RS23290"
                     /old_locus_tag="pgaptmp_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU4561005.1"
                     /translation="YLDNMEATGFYRISLPSAQPGDILLCCFGASVPNHAAIYCGNGE
                     LLHHLPEQLSKRERYSEKWQRRTHSAWRHRHWHASAFTGIYNDLAAASACM"
     gene            285..866
                     /locus_tag="ACHOSM_RS23295"
                     /old_locus_tag="pgaptmp_004659"
     CDS             285..866
                     /locus_tag="ACHOSM_RS23295"
                     /old_locus_tag="pgaptmp_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4561006.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGYDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGTVVVVGRQG"
     gene            1209..4700
                     /locus_tag="ACHOSM_RS23300"
                     /old_locus_tag="pgaptmp_004660"
     CDS             1209..4700
                     /locus_tag="ACHOSM_RS23300"
                     /old_locus_tag="pgaptmp_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU4561007.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRILSIDASSRTLTLD
                     REVTLPETGAATVNLINGSGKPVSVAITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGASFEPQSGTLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAVPATIELTPGYFQITAVPRLAVYDPTV
                     QFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKSAF
                     VEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSNEI
                     TQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTP
                     DGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPA
                     FSLTPDGRLTAKNADISGSVNANSGALNNVTINQNCTIKGMLEATQVRGDFVKAVSKA
                     FPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRY
                     TGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNIT
                     DCTVIVTKKAASGISIR"
     gene            4771..>5339
                     /locus_tag="ACHOSM_RS23305"
                     /old_locus_tag="pgaptmp_004661"
     CDS             4771..>5339
                     /locus_tag="ACHOSM_RS23305"
                     /old_locus_tag="pgaptmp_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4561008.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDRNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPIESVAIDIAYEGSGSGDWRTDG"
BASE COUNT         1275 a         1349 c         1613 g         1102 t
ORIGIN      
        1 tacctggaca atatggaggc gactggtttt tacaggattt ccctgccttc cgcacagcct
       61 ggcgatattc tgctgtgctg ctttggtgct tcggtaccga accatgccgc catttactgc
      121 ggcaacggtg agctgcttca ccatctgcct gaacaactga gtaaacggga gaggtattcc
      181 gaaaaatggc aacgacgaac gcattcagcc tggcgtcacc gccactggca cgcatctgcc
      241 ttcacgggga tttacaacga tttggccgcc gcctcagcct gtatgtgaac acggcagcgg
      301 aagccatccg ggcgctgtcg ttacaggtgc cgggctttcg ccgtcagatg aacgaaggct
      361 ggtaccagat acgtattgcc ggttatgaca cggcaccgga ggcggtgtac gcccgtcttc
      421 acgaacagct gggtgaggga acggtcatcc atattgtgcc gcgactggcc ggggccggaa
      481 agggtggact gcagattgtg ctgggggcgg cagccatcgt gggctctttc ttcactgccg
      541 gggcatcaat ggcgttatgg ggttcagccc tggcagccgg tggtttttct gccaccacga
      601 tgctgttttc acttggagcc agcatgattc tgggcggtgt ggcccagatg ctggccccga
      661 aggcaaaaac accggagtac aaaagcacgg ataacggcag acagaacacg tacttttcct
      721 cgctggataa catgattgcc caggggaacc cgatgccggt gccttacggg gaaatgctgg
      781 ttggctcccg ccgtatatcc caggacatca gcacccgtga tgaaggcggg ggcggaacgg
      841 tcgtggttgt cgggcgacag ggataaaaca taaaaaaatc ccgcagtgat cgcggagctg
      901 cggggacaga caaatgaaga tcaatgttaa ggagttgttt ttgttactcg ggcaaaaaaa
      961 cactaacgca gcgaaattat aagcgccaca gtcagtgtgt gaaaatgtga agatattcag
     1021 aatttttatg ccattaccgg ttttaaccaa caggattatc ggtgggcatg aaagaaaacc
     1081 ccggtatctg ctgataccgg ggtttctctt tagcatggca gaaatgtgtt tcatgctttt
     1141 cgggcgaagg atatccgact tctgtacgga atggcaagtg gcggttaatt tattcagggg
     1201 aaggctgtat gggaaaaggt ggcggtaagg cacacacgcc tcgtgaggcg aaggataatc
     1261 tcaaatccac gcagatgatg agtgtgattg atgcgattgg tgagggaccg atagaaggtc
     1321 cggtgaaggg actgcagagt attctggtga acaaaacccc actgacggac acggacggca
     1381 atcccgtgat acacggtgtg accgcggtct ggcgtgccgg ggagcaggag cagacaccac
     1441 cggaaggctt tgagtcctcc ggagctgaaa ccggactggg cgtggaagtg acgaaggcaa
     1501 aaccggtgac gcgcaccatt acgtccgcga acattgaccg cctgcgggtt accttcgggg
     1561 tgcagtcact ggtggagacc acctcaaagg gtgaccgtaa cccggcatcc gtccgcctgc
     1621 tgattcagtt acagcgtaac ggtaactggg tgacagaaaa ggacgtcacc attaacggca
     1681 agaccacctc acagttcctg gcctcggtga ttctggataa tctgcctccc cggcccttta
     1741 acatccggat ggtcagggag acggcggaca gcaccacgga ccagctgcag aataagacgc
     1801 tgtggtcgtc atacaccgaa atcatcgatg tgaaacagtg ctacccgaac acggccattg
     1861 tggggctgca ggtggatgcg gagcagttcg gcggccagca gatgacggtg aactaccata
     1921 tccgcggtcg catcatccag gtgccgtcaa actatgaccc ggaaaaacgc acgtacagtg
     1981 gtatctggga cggcagtctg aaaccggcat acagcaacaa cccggcctgg tgtctgtggg
     2041 acatgctgac tcacccgcgc tacggcatgg gaaaacgtct gggggcggcg gatgtggaca
     2101 agtgggcgct gtatgccatc gggcagtact gcgaccagac ggtcccggat ggtttcgggg
     2161 ggaccgagcc gcggatgacc tttaatgcgt acctggcaca acagcgtaag gcgtgggacg
     2221 ttctcagtga tttctgctct gcgatgcgct gtatgccggt atggaacggt cagacgctga
     2281 cgttcgttca ggaccgcccg tcggatgtgg tgtggccgta caccaacagc gatgtggtgg
     2341 tggatgataa cggcgtggga ttccgctaca gcttcagtgc cctgaaggac cggcacacgg
     2401 cggtggaggt gaattacacc gacccgcaga acggctggca gacctccacg gaactggtgg
     2461 aagacccgga agccatactg cgctacggac gcaacctgct gaagatggac gcgttcggct
     2521 gtaccagccg cggtcaggcc caccgtgccg ggctgtgggt gataaagacc gaactgctgg
     2581 aaacgcagac ggtggatttc acgctcgggt cacaggggct gcgtcacaca cccggtgaca
     2641 ttattgaaat ctgtgataac gactatgccg ggaccatgac cggcggacgt atcctgtcca
     2701 tcgatgcctc cagccgcacc ctgacactgg accgtgaggt gaccctgccg gagacaggtg
     2761 ccgccacggt gaacctgatt aacggcagcg gtaagccggt gagcgtggcc atcactgcac
     2821 accccgcgcc ggaccggata caggtcagca ccctgccgga tggtgtggag acatacggtg
     2881 tatggggact ctccctgccg tcactgcgtc gtcgcctgtt ccgctgtgtc tccatccggg
     2941 aaaacacgga cggcaccttt gccatcacgg cggtgcagca cgtaccggaa aaagaagcca
     3001 tcgtggataa cggggccagc tttgagccgc agtcaggcac cctgaacagc gttattccac
     3061 cggcagtgca gcacctgacg gtggaggtga gcgcggctga cggtcagtat ctggcacagg
     3121 cgaaatggga cacgccgcgg gtggtgaagg gtgtgcgctt cagtctgcgc ctgaccagcg
     3181 gaagcggaga agacagccgt ctggtgacca ccgccatcac tgcggataca gagcatcgtt
     3241 tcagtggtct gccgctcggg gaatacaccc tgacggtcag ggcgattaac agttatggcc
     3301 agcaggggga accggccacc accacgttca ggattaatgc acctgcggta cccgccacga
     3361 ttgagctgac accgggctat tttcagataa cggcggtccc gcgtcttgcg gtgtatgacc
     3421 cgacggtaca gtttgagttc tggttctcgg aaacgcggat taccgatatc aggcaggttg
     3481 aaaccacagc ccgctatctt ggcacggggc tgtactggat agccgccagt atcaatatca
     3541 aaccgggcca tgattattac ttttatatcc gcagtgtgaa caccgttggc aaatcggcat
     3601 ttgtagaggc tgtcggtcag ccgagtgatg atgcatcagg ctatctggat tttttcaaag
     3661 gcgagatagg gaaaacccat ctggctcagg agctgtggac gcagattgat aacggtcagc
     3721 ttgcgcctga cctgactgaa atcaggacgt ccataacgga tgtcagcaat gaaataacac
     3781 agaccgtcaa taagaaactg gaagaccaga gtgcagcgat ccagcagata cagaaggttc
     3841 aggttgatac aaataataac ctgaacagca tgtgggcagt gaagctgcag cagatgcagg
     3901 acggacgcct ttatattgcg ggtatcggtg ccggtattga gaacaccccc gacggcatgc
     3961 agagtcaggt gctgctggca gcagacagga ttgcgatgat taatcctgcg aatggcaaca
     4021 caaagccgat gtttgttggt cagggcgatc agatattcat gaatgaagtg ttcctgaaat
     4081 atctgacggc tcccaccatt accagcggcg gtaatcctcc ggcattttcc ctgacaccag
     4141 acgggcgact gacggcgaaa aatgcggata tcagtggcag tgtgaatgcg aactcaggag
     4201 cgctcaacaa tgtcacgatt aaccagaact gtacgattaa gggcatgctg gaggcgaccc
     4261 aggtcagagg ggatttcgtt aaagctgtat caaaagcctt cccgaaaaaa gtcggtacgt
     4321 ggggtaacac ggaaacacca aacggtacgg ttacagtcac catcagcgat gatcataact
     4381 ttgaccgcca gattattatt ccgcccatta tttttaacgg tatagcgtat gacgatccgg
     4441 ggagcggaaa taacccagga ggcacgcgat acacgggtta tggttttgaa gttcgcaaaa
     4501 acggcgtatt aatcgcatcc agagaaacta aaggggccat tcccggtagt tacagtgcag
     4561 ttattgatat gcctagtggt ggtggtagcg tcactctgga gtttaagatt ttccagaaag
     4621 gcaatcaggg ggcaggcaat atcaccgact gtacggtgat tgtgaccaaa aaagctgctt
     4681 ccggcatcag tattcgttga aatatttata accccaataa agggcgtcag gaatgacgcc
     4741 ttttttattg cagaaaagcg agaggtaatt atgcgtaaac tttatgccgc cattttgtcc
     4801 gcagccattt gtctggccgt atccggtgcg cctgcatggg cgtctgaaca tcagtcaacg
     4861 ctgagcgcgg ggtatcttca tgtctcgacg aacgttcctg gcagcgatga actgaacggg
     4921 attaacgtga aataccgtta tgagtttacg gacacactgg ggatggtgac gtcgttcagc
     4981 tatgcaggag acaggaatcg ccagctgacc cgttacagcg atacccgctg gcatgaagat
     5041 tccgttcgta accgctggtt cagcgtaatg gcggggccgt ctgtgcgcgt gaatgaatgg
     5101 ttcagcgcgt atgcgatggc gggtgtggct tacagccgtg tgtcgacttt ctccggggat
     5161 tatctccgcg taactgacaa caaggggaaa acgcatgatg tgctgaccgg aagtgatgac
     5221 ggtcgccaca gcaacacgtc tctggcgtgg ggagctggcg tgcagtttaa cccgatcgaa
     5281 tccgtggcca ttgatattgc ttatgaaggc tccggcagtg gcgactggcg cactgacgg
//
LOCUS       DAZKHC020000193         5021 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZKHC020000193 DAZKHC020000000
VERSION     DAZKHC020000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.194"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /locus_tag="ACHOSM_RS23310"
                     /old_locus_tag="pgaptmp_004662"
     CDS             <1..198
                     /locus_tag="ACHOSM_RS23310"
                     /old_locus_tag="pgaptmp_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4561009.1"
                     /translation="WGISGEAELAVMQAIAEKPGALRVLTHTLNQAWLTASGEGAALT
                     EKHINAAFKEVYTNPELLSQV"
     gene            201..422
                     /locus_tag="ACHOSM_RS23315"
                     /old_locus_tag="pgaptmp_004663"
     CDS             201..422
                     /locus_tag="ACHOSM_RS23315"
                     /old_locus_tag="pgaptmp_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561010.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKAVSMSDSEECGDLLFEFNDRTR
                     MAISAGLDREGAKEAYSNICRFIGARRVS"
     gene            435..854
                     /locus_tag="ACHOSM_RS23320"
                     /old_locus_tag="pgaptmp_004664"
     CDS             435..854
                     /locus_tag="ACHOSM_RS23320"
                     /old_locus_tag="pgaptmp_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561011.1"
                     /translation="MKMQIQQEINVLHSFREFNNNDCEVRDYIYELMNGDGVCFLFID
                     LYQWYADQQCESVRQRFDYECFDVDVWEGLRFGFLIPGDYRREWRHSYSILRAIMRGE
                     TRKMPGMGKVNRGLLLEIAAAHMNPLLIQPEFTYEFV"
     gene            841..1071
                     /locus_tag="ACHOSM_RS23325"
                     /old_locus_tag="pgaptmp_004665"
     CDS             841..1071
                     /locus_tag="ACHOSM_RS23325"
                     /old_locus_tag="pgaptmp_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561012.1"
                     /translation="MNLFDSLNNARRLTELAGAVLERSKRYPQRFALKTTPPVGEVQG
                     SGEEIEITVKTNGLRRRVKATRISGCTVYWEV"
     gene            1146..1418
                     /locus_tag="ACHOSM_RS23330"
                     /old_locus_tag="pgaptmp_004666"
     CDS             1146..1418
                     /locus_tag="ACHOSM_RS23330"
                     /old_locus_tag="pgaptmp_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561013.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKARALMKDMNMSSGVIKGNEFNQQG"
     gene            1423..1716
                     /locus_tag="ACHOSM_RS23335"
                     /old_locus_tag="pgaptmp_004667"
     CDS             1423..1716
                     /locus_tag="ACHOSM_RS23335"
                     /old_locus_tag="pgaptmp_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561014.1"
                     /translation="MSKVRVIFEFEHVSHDEKPAGNDSVEVHEKIGVDVKTERDTDNR
                     PTSLCDVYASILQYHSPVIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            1727..2257
                     /locus_tag="ACHOSM_RS23340"
                     /old_locus_tag="pgaptmp_004668"
     CDS             1727..2257
                     /locus_tag="ACHOSM_RS23340"
                     /old_locus_tag="pgaptmp_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4561015.1"
                     /translation="MVKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIHDWCKTNRNELTNGGETKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKQAVLAEPDAVKGIAGIKVNKGA
                     ESFHVEPFEQDAGLNK"
     gene            2342..2920
                     /locus_tag="ACHOSM_RS23345"
                     /old_locus_tag="pgaptmp_004669"
     CDS             2342..2920
                     /locus_tag="ACHOSM_RS23345"
                     /old_locus_tag="pgaptmp_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HGU4561016.1"
                     /translation="MSIKYRYFKMNECDSRHYHREWLDCAGRERKKLIDDFLSNHRAK
                     GYLYFWCCGHIFVTSILVHEDVDVGRNKRVLSDKFDEDGRVLFSVKPDRRFREGKKLN
                     KALNILNEKLKQLPSFSTWMVNKLDCYFEVFGVSNGRTVMACSSAGFYGDKGAVVVRI
                     PVGESDNAFSPEKLHPSLMAIKHSEFIAITEE"
     gene            2924..3457
                     /locus_tag="ACHOSM_RS23350"
                     /old_locus_tag="pgaptmp_004670"
     CDS             2924..3457
                     /locus_tag="ACHOSM_RS23350"
                     /old_locus_tag="pgaptmp_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561017.1"
                     /translation="MIDAKVLEGVKNWLRIYGRLTCGVLAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQESAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITGHVLRY
                     LIVDRTAAPAGISAEVA"
     gene            3457..3972
                     /locus_tag="ACHOSM_RS23355"
                     /old_locus_tag="pgaptmp_004671"
     CDS             3457..3972
                     /locus_tag="ACHOSM_RS23355"
                     /old_locus_tag="pgaptmp_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561018.1"
                     /translation="MFFKTSNPSALAAWQKYQQDYKKVKDEAKRLEAVLNVDCRSVFV
                     SGINGFYFKGLRFTDNKYPFHRDLWRKPTASNGWSCTPRTSCIPKALRAASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAIMFGPVKWFCVDDVIYLECGVKPANQKMTEILSD
                     EFYAAEKRVRG"
     gene            3976..4527
                     /locus_tag="ACHOSM_RS23360"
                     /old_locus_tag="pgaptmp_004672"
     CDS             3976..4527
                     /locus_tag="ACHOSM_RS23360"
                     /old_locus_tag="pgaptmp_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HGU4561019.1"
                     /translation="MMILQPMGRKGQAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLLFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAEDVVLRELMP"
     gene            4524..4856
                     /locus_tag="ACHOSM_RS23365"
                     /old_locus_tag="pgaptmp_004673"
     CDS             4524..4856
                     /locus_tag="ACHOSM_RS23365"
                     /old_locus_tag="pgaptmp_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561020.1"
                     /translation="MITTLFVESDEPLVCAAGMPVCGGTMTGVYFGDLRGYPWHSLDD
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFVDEETGACLEDEDGQVTHWCHISSLP
                     ELQETPNG"
BASE COUNT         1337 a         1021 c         1359 g         1304 t
ORIGIN      
        1 tggggaatca gtggtgaggc cgagctggcc gtcatgcagg ctatcgctga aaagccggga
       61 gcgttacgcg ttctgacaca tacgcttaac caggcgtggc tcaccgccag cggtgaaggc
      121 gcggcgctga cagaaaaaca tattaatgcg gcctttaaag aggtttatac caaccctgaa
      181 ttactctcgc aggtgtgatt atggctgtat ttaatattcc tgatatttac ggacgctttt
      241 acctggttaa tttcgataac gtcaaagcag tctccatgtc tgacagtgaa gaatgtggcg
      301 atttactctt tgaatttaat gatcgcaccc gaatggcaat atcggcagga cttgatcgtg
      361 aaggtgcgaa agaagcttac agcaatattt gtcgctttat cggtgcaaga cgggttagtt
      421 aaacgagagg ttatatgaaa atgcagatac agcaggaaat caatgttctc cacagctttc
      481 gtgagtttaa caacaatgac tgtgaggttc gtgattatat ctatgaattg atgaacggtg
      541 acggtgtgtg ttttctgttt atcgacctgt atcaatggta tgcagaccag cagtgtgaat
      601 ctgtccggca gcgttttgat tatgagtgct ttgatgtgga cgtatgggaa ggcctgcgtt
      661 ttggttttct gatcccgggt gattatcgcc gggagtggcg acatagttac tccattcttc
      721 gggcaatcat gcgtggcgaa acccgaaaaa tgccggggat gggtaaggtt aatcgcggcc
      781 tcctgttaga gatagcagcg gcgcacatga atccattact gatacaaccg gagtttactt
      841 atgaatttgt ttgattcact gaataacgcc cgtcgcctga ccgagcttgc tggtgcggta
      901 ctggaacgca gtaagcgcta tccgcaacgt tttgcactga aaaccacgcc gccggtaggc
      961 gaggttcagg ggagtggtga agaaattgaa atcaccgtta agaccaacgg cctgcgccgc
     1021 cgtgtgaagg ccacccgcat aagcggatgc acggtttact gggaagtatg aagttacttc
     1081 atggttcggc tctgtcatca ggccagacgc cgggtcagtt atccgaacaa aacgaggacc
     1141 agaggatgag taaagtcgta cgcattattt tcgaatacaa ggagcacgtt atccataaaa
     1201 acgctgatgg aacagtgcgc atgggggtaa gtctggacat acgttcaacc gggataaagc
     1261 agaaaggtga tggacccgcc atgatttttg gagtggttat gctcgcggaa agcagagact
     1321 ttgctgaact tgttgcaatg aaagccagag cgctcatgaa agatatgaac atgagttccg
     1381 gggttattaa aggtaatgaa tttaatcagc aggggtaatt ccatgagcaa agtacgcgtt
     1441 atttttgaat ttgagcatgt ttcgcatgac gaaaaaccgg caggcaatga cagtgttgaa
     1501 gtgcatgaaa agattggagt ggatgtgaaa acagaacgtg atacggataa caggccgacg
     1561 tcactctgtg acgtttatgc aagcattctc cagtatcaca gtcctgtaat tattcagttt
     1621 ctctcagcgg aatttcaggc atctgtacag gcttttgggg cggatgccat cattaaacgc
     1681 caccgtgtgc ataaagcatc aggcacactg caataaggaa aacaaaatgg ttaaacgtgt
     1741 tacaaaatta aaggccgcag ccgaggctgc accgcagacc cgcgaagaag tcagccgcga
     1801 tatccgcacc ctgggcgata ttcagcgaga ggcgctgcgc ctggaaacgg caatgaatga
     1861 tgaagtggca gaaatcaccg cccgttatac gccgcagatt gaaaacctta aaaaagaaat
     1921 caaagtgctt tttaaaggga ttcatgactg gtgtaaaacc aaccgcaatg agctgacgaa
     1981 cggcggcgaa accaaaactg ccaatctgac caccggaacg gtgtcatggc ggctgggaaa
     2041 tccatcatgc agcgtcagtc gtgatgtgga aggtgtgatt gaaatgctgc gtcgtatggg
     2101 gcttgagcgc ttcatccgca cgaaagagga agtgaataag caagctgtcc tggcagagcc
     2161 ggatgcggtg aaagggattg ctggtattaa ggtgaataaa ggcgctgaaa gttttcatgt
     2221 cgagcctttt gaacaggacg ccggactgaa taaataacac cgcattaaat ctttaaatat
     2281 cacatcgttt taattatggc gcttgcgtca ggggactgct cgcgcctgaa tgaggcatat
     2341 catgagtata aaatatcgtt attttaaaat gaatgagtgc gattcccgtc attatcacag
     2401 ggaatggctt gattgcgctg gcagggagcg caagaaattg attgatgatt ttctcagtaa
     2461 tcatagagct aaaggatacc tttacttctg gtgttgtggg catatttttg tcacatcaat
     2521 actggttcat gaggatgttg atgtcggaag aaataagcgg gtgctgtctg ataaatttga
     2581 tgaagatggc cgcgttttat tttcagttaa gcccgaccgc agattcagag agggaaaaaa
     2641 actgaacaaa gcactgaata tcttaaacga gaagttaaaa cagcttccgt cattcagtac
     2701 ctggatggtt aataaactgg actgctattt tgaggttttt ggtgtcagca acggccgcac
     2761 agttatggcg tgctcttctg ccgggttcta tggcgacaaa ggggctgttg tggttcgtat
     2821 tcctgtcggt gaatcagata atgccttttc ccctgaaaag ctgcatccgt cattgatggc
     2881 aatcaagcat tccgaattca tcgcaataac ggaggaataa ttcatgattg atgcaaaagt
     2941 gcttgaaggg gttaaaaact ggctgcgtat ttacggacgt ctgacctgcg gtgttctggc
     3001 tgaaaaaatg aatatgccgc catcctccat ggtttatttt ctgcgtgatg cggttgatgc
     3061 aggcgtgctg acggaatgta acggttttta tgatattccg cgtccccgcc cggtgcagcc
     3121 ggttcgtcgc aaatgcagcc aggaatctgc ggctgatgat gttcagtggt gcagcttcag
     3181 aaaatccctg ccgtggattg aagggcatga tattccgtcg atggcgtggg aatttgctca
     3241 gggcgtactg acctgtgaaa ccgtttatgt ggtggctgaa gttgatgagc aggccatgaa
     3301 agaaggtgtg ccccagtttg ttatggcgta tatcgacatc cgtctgggcg tcattatctg
     3361 cggtttaagc ggctggaata tcaccggaca tgttctgcgt tacctgattg ttgaccggac
     3421 ggccgcgcct gccgggatat ctgcggaggt ggcgtaatgt tctttaaaac atcaaaccct
     3481 tccgcgctgg ctgcgtggca aaaataccag caggactaca agaaagttaa ggatgaggca
     3541 aaacgtcttg aggccgtgct gaatgttgat tgtcggtcgg tatttgtatc cggtatcaat
     3601 ggtttttatt ttaagggact gcgttttacg gataacaaat atccttttca tcgcgactta
     3661 tggcgaaaac cgactgcgtc gaatggctgg agctgcacgc cgcgcacatc gtgtattccc
     3721 aaagccctgc gcgctgcctc tgacgaactt aacagtctgt ggcgtgaata ttcgcccgtc
     3781 acgtatgcca gaaccgatgc actgttgttc tggctgggta ttgatttctc ggcaatcatg
     3841 tttggccctg tgaagtggtt ctgcgttgac gatgtgattt accttgagtg cggcgtaaaa
     3901 cccgcaaatc agaaaatgac cgaaattctg tctgatgagt tttatgctgc tgaaaagcga
     3961 gtcagggggt gatgtatgat gattttacaa cccatggggc gaaaaggtca ggcacctgct
     4021 catgtccgtg cgtggacacc tgaagaagat gcgctgctga ttgcgcttta tccatccacc
     4081 ccggttaagg atattgctgt cagggtaaaa agaagtttct ggggtgtaca taaccggatt
     4141 gttttattac gcggtactta cccggagttg ctcaaatgca aacgtccaag atttaaacat
     4201 gatgaagata aatttatccg aaaaaatgcc agaacgatga ccggtaagca aataggagaa
     4261 tatctggggc gagaccggga ttctgtccac aatcgggcgc gatatattgg cgtaagcatg
     4321 aaaaaatacg gagaattgct gctcttcacc cgtatacctg acgatgatgt tcatcttatt
     4381 cgtgaattac gggatgctga atcaccacga cgtcttacct tccgggaaat aggcgagaaa
     4441 tttgaattat ccgagagtac ggtgaatttt atttatcacc atcgccggac tgccgaagat
     4501 gttgtattac gggagttaat gccatgataa cgacattatt tgttgaatca gatgaacccc
     4561 ttgtgtgtgc cgccggaatg ccggtctgtg gcggaacgat gaccggtgtt tatttcgggg
     4621 atttgcgcgg ttatccctgg cattcactgg atgacgcatt tccgcctgat atggaggctg
     4681 tcgtgctgat tgttcagtat ggtgaccaac aggaactgcg catcggccat atggggtatg
     4741 aaggcttttt tgttgatgaa gaaaccggag cctgccttga agatgaagac ggacaggtga
     4801 cgcactggtg ccatatttca tctttaccgg aattacagga gacgcccaat ggatgatcaa
     4861 atagttgaat gtcccacctg tggaaatgaa gccccggagt atctgaaaga gtgcccacat
     4921 tgtggtgagg taaaatgtaa tcactgcgat atgggcgacg acactgcatg tatgaattgt
     4981 gaggattaat aatatggaaa aagaaatcaa atttgcgcct a
//
LOCUS       DAZKHC020000194         4897 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZKHC020000194 DAZKHC020000000
VERSION     DAZKHC020000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.195"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            44..199
                     /gene="hokD"
                     /locus_tag="ACHOSM_RS23370"
                     /old_locus_tag="pgaptmp_004674"
     CDS             44..199
                     /gene="hokD"
                     /locus_tag="ACHOSM_RS23370"
                     /old_locus_tag="pgaptmp_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU4561021.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            319..1188
                     /locus_tag="ACHOSM_RS23375"
                     /old_locus_tag="pgaptmp_004675"
     CDS             319..1188
                     /locus_tag="ACHOSM_RS23375"
                     /old_locus_tag="pgaptmp_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561022.1"
                     /translation="MTDQQVTELEIAYAGDAYANNEIDAKMLGDALSSLSNLIDNAEK
                     IIHGETATTRVSIKAIQEGSFTVLVSVLGSLSTLQLLGLSVATGASAGSLVGVVEWLR
                     GRKPHEVVIDEANNRATFVVDNERFECSNDVQKLVTNSTIRNEMSKLVHTPLQTEKNS
                     SFRVKNSSGEEIVSISQEQSVSFKKVNNTVVQHTHEDSLTLNVKFSKLSFTSDTDWRM
                     VLPDGEDVKVKMADAGFLERVNLNEASFSKDDLFVVNVTKRVKETNGVLGNPAYIITQ
                     VVRHRAAAERRIV"
     gene            1433..1825
                     /locus_tag="ACHOSM_RS23380"
                     /old_locus_tag="pgaptmp_004676"
     CDS             1433..1825
                     /locus_tag="ACHOSM_RS23380"
                     /old_locus_tag="pgaptmp_004676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561023.1"
                     /translation="MIQQSKYVASQAWCTPVANYLLVKASAYIGLPADINAFLPGVAV
                     VIAHFFLLLLTFVNVPSIEDVRFKVDVARARKTLTKMRADASDEEKVSIDNALATLRS
                     KQIEKTLRDVDYSMELHKHSLKNDIESM"
     gene            2019..2096
                     /locus_tag="ACHOSM_RS23385"
                     /old_locus_tag="pgaptmp_004677"
                     /pseudo
     CDS             2019..2096
                     /locus_tag="ACHOSM_RS23385"
                     /old_locus_tag="pgaptmp_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2252..2851
                     /locus_tag="ACHOSM_RS23390"
                     /old_locus_tag="pgaptmp_004678"
     CDS             2252..2851
                     /locus_tag="ACHOSM_RS23390"
                     /old_locus_tag="pgaptmp_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HGU4561024.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPNGGAITPRERELVSGFVDFLCESVGREHTPALSDAAEQ
                     YLNTVTTCRTRDTALLKSFDAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
     gene            2851..3141
                     /locus_tag="ACHOSM_RS23395"
                     /old_locus_tag="pgaptmp_004679"
     CDS             2851..3141
                     /locus_tag="ACHOSM_RS23395"
                     /old_locus_tag="pgaptmp_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HGU4561025.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACNSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKDGYL"
     gene            3138..3674
                     /locus_tag="ACHOSM_RS23400"
                     /old_locus_tag="pgaptmp_004680"
     CDS             3138..3674
                     /locus_tag="ACHOSM_RS23400"
                     /old_locus_tag="pgaptmp_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HGU4561026.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     IIDRVIGEVLADHPGLLNVLSQRYVGRGMSKRRMAELLNEQYPEWALITCRRRVDQWL
                     SIAEFILYSPMREAFDYA"
     gene            3832..3907
                     /locus_tag="ACHOSM_RS23405"
                     /old_locus_tag="pgaptmp_004681"
     tRNA            3832..3907
                     /locus_tag="ACHOSM_RS23405"
                     /old_locus_tag="pgaptmp_004681"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3865..3867,aa:Ile,seq:cat)
     gene            3917..3993
                     /locus_tag="ACHOSM_RS23410"
                     /old_locus_tag="pgaptmp_004682"
     tRNA            3917..3993
                     /locus_tag="ACHOSM_RS23410"
                     /old_locus_tag="pgaptmp_004682"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Arg,seq:tcg)
     gene            4007..4083
                     /locus_tag="ACHOSM_RS23415"
                     /old_locus_tag="pgaptmp_004683"
     tRNA            4007..4083
                     /locus_tag="ACHOSM_RS23415"
                     /old_locus_tag="pgaptmp_004683"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4041..4043,aa:Arg,seq:tct)
     gene            4614..>4897
                     /locus_tag="ACHOSM_RS23420"
                     /old_locus_tag="pgaptmp_004684"
     CDS             4614..>4897
                     /locus_tag="ACHOSM_RS23420"
                     /old_locus_tag="pgaptmp_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4561027.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNESKAVAVSATSDPEYYFVVVLAGQSNGMSYGEG"
BASE COUNT         1305 a         1030 c         1271 g         1291 t
ORIGIN      
        1 taggttagcc tcttacgcgc cgaaaggcaa ggagaagcag gctatgaagc agcaaaaggc
       61 gatgttaatc gccctgatcg tcatctgttt aaccgtcata gtgacggcac tggtaacgag
      121 gaaagacctc tgcgaggtac gaatccgaac cggccagacg gaggtcgctg tcttcacagc
      181 ttacgaacct gaggagtaag agaccaggcg gggtaaaccc ccgccacctc tgatgtgtca
      241 ggcatcctca acgcacccgc cataaagtga aaatactgtt aattattttg catatttttt
      301 atggtggagt tgaacttcat gaccgaccaa caggtaacag agcttgagat tgcatatgct
      361 ggggatgcat atgcgaataa tgagatcgat gctaaaatgc ttggtgatgc tctgtcttca
      421 ttaagtaatt tgattgataa tgcagagaaa attatccacg gtgagactgc aacaactagg
      481 gttagtatta aggcaattca ggaaggatct tttactgttc ttgtctctgt tttgggtagt
      541 ttatctactt tgcaactctt ggggctttct gttgccaccg gcgcaagtgc tgggagtttg
      601 gttggggttg tagaatggtt gcgagggcga aagccacatg aggttgttat agatgaagcc
      661 aataaccgag cgacttttgt tgttgataat gagcgatttg agtgttcaaa tgacgtacaa
      721 aagctcgtta caaactctac gatccgcaat gaaatgagta aattggtaca cactcctctg
      781 caaactgaaa aaaactcgtc gtttagagta aaaaactcaa gtggggagga gattgtctct
      841 atttcgcaag agcaatctgt ttcttttaaa aaagtgaata atacggttgt acaacataca
      901 catgaagact cattgacctt aaacgtcaag ttttctaagc tgagctttac ttccgataca
      961 gattggagga tggtattgcc agacggcgag gatgtaaagg ttaagatggc ggatgctggt
     1021 tttcttgagc gagtaaattt aaacgaagcg tcattcagta aagatgatct ttttgtagtg
     1081 aatgtgacaa aaagagtcaa agaaactaat ggtgtattgg ggaatccagc atacattatc
     1141 acgcaggttg tacggcatcg cgccgcagct gaacgtcgaa tcgtataggt aataatatgc
     1201 cttatttaag tgtcatgttg atgattacgg tttttgttgc cgcctttatg ttggctcgtt
     1261 ttgctgtata tcattttttg gtttggctta aaccaagtaa gacactcaaa tttacttata
     1321 tagatcgatg tggagttagg cattattgcg aagttcagtt ggatgcaaag gatgcacgtg
     1381 agctcgcgtc cacgttgagt gaggttcagc gtgatttaac gcgaggcact tcatgattca
     1441 acaatctaag tatgttgcaa gccaagcgtg gtgcacaccg gtggcaaatt acctattggt
     1501 caaggctagc gcatatattg ggttacctgc tgatatcaac gcatttctcc ctggggttgc
     1561 tgttgttatt gctcacttct ttttgctact tcttacgttt gttaacgtac ctagcataga
     1621 ggacgtacgt ttcaaggtgg atgtggcaag ggctcgtaag actcttacga aaatgagagc
     1681 tgatgcttca gatgaagaaa aagtgtcaat tgataatgcg cttgccaccc tgagaagcaa
     1741 gcagattgaa aaaactcttc gtgatgttga ttactccatg gagttacaca agcactcact
     1801 aaaaaatgac attgagagta tgtaatatac aaaaaacccg cttcggcggg ttttttactt
     1861 taacagatca agttttagcc gattcctcca ctaaaccgac cttcgtttgt tgataagcgg
     1921 gtatgcctga ctagaatcga tgcacttaag tagcgcgcag ggagaagagg gatggacccc
     1981 gagcagggga gtgctattta tctggaagga ttctgttgat gaaaatcgaa gaattgcgtg
     2041 aaatttttag tgaaaatggc ctctatgctg tgcgcgttga gaatggggaa gttatctacg
     2101 caacgttaat ccctgataat catgtaattt tatctatcga ggcattcatt gaatatcttg
     2161 aaaggctcgg tttcaaggtg attcgggaat gagttataat tcgtaagcca gcctgaacaa
     2221 ctggcaacct acagcgccat tggagacagc aatggcgcat atacaactgg tcaaacaaac
     2281 ctcttccgga ttacttctcc cggcgacgcc ggagagttgc gattttttgc atcaaatcaa
     2341 aataggtgag tggatacacg cggactttaa gcgagtgcgt aactacgcgt tccacaagcg
     2401 ttttttcaaa ctcctgcaac tgggattcga ttactggact ccgaacggtg gggcgatcac
     2461 gcctcgcgaa cgagaactgg tgtccggttt cgttgatttc ctgtgcgaat cagtaggtcg
     2521 ggaacatacg ccagccctga gtgatgccgc agagcaatac cttaacaccg ttacgacttg
     2581 cagaacccgg gatacggcct tactcaagtc ctttgacgct tttcgcgaat gggtaaccat
     2641 tcaggccgga ttttacaccg aacattttta tccggacggt agtcgtgggc gcagggcaaa
     2701 atctatcgca tttgcgaaca tggacgaaac cgagtttcag caggtttata aatctgtact
     2761 gaatgtgctg tggaactgga tcctgttccg taaattttcc tctccggagg aagtcgaaaa
     2821 tgtggccgcg cagttactgg agtttgcgta atggtggatt tacgtaaagc ggcgcggggg
     2881 cagatgtgca cagtcagaat tcctggctac tgcaatcaca atcccgaaac ttctgtgctg
     2941 gcgcattaca ggctggcggg gacgtgcgga acagcgacaa aaccacacga tatgcaggcg
     3001 gcgattgcct gtaactcatg ccacgattta atcgacggga gagtaaaaac cagcgattac
     3061 accaaagaag aattgcgcct gatgcatgca gaaggtgttt ttcgcacaca agagatctgg
     3121 agaaaggacg gttatttatg atttacccaa cgaacacagg aaaaagcgga gagcaccttc
     3181 gtctcaccac gctggaaagt gtctggattc agggaaaact gcgcatgtgg gggcgctggt
     3241 cgtatattgg cggcggtaag acggggaata tgttcaacca gttgttggcc tctaaaaagc
     3301 tgacaaaaac ggcaattaac gaggcgctcc ggaggatgaa aaaagcaggt ctgaacaagt
     3361 ctgaacttga ggcttttttg cgggatatga ttaacggtaa gcaaaagagc tggctggcgc
     3421 attgtactga tgcagaggca ttaataatcg acagggttat tggcgaagta ctggcggatc
     3481 atcccgggct gcttaatgtc ctgagtcagc gttatgtggg acgagggatg agtaagagaa
     3541 ggatggccga gctactaaac gaacagtacc cagagtgggc gttgattaca tgccgacgcc
     3601 gtgttgatca gtggttgagt attgctgagt tcattttgta ttcacctatg agagaagcgt
     3661 tcgattatgc ttaaaaaatc attgcaaaat gagccacaaa ctgcttcaat tccggtaagc
     3721 ttcgcaaagc tgtatcgcga ggcgaaccaa acgcatgaac tttaccagaa cccgccattg
     3781 agcgggtttt ttattgcgga attaattacg gaccgttatt attctgctcc cggcccttta
     3841 gctcagtggt gagagcgagc gactcataat cgccaggtcg ctggttcaaa tccagcaagg
     3901 gccaccatat cacataccgc cattagctca tcgggacaga gcgccagcct tcgaagctgg
     3961 ctgtgcgggg ttcaagtccc cgatggcggt ccattatcag catcatgcgt tgttagctca
     4021 gccggacaga gcaattgcct tctaagcaat cggtcactgg ttcgaatcca gtacaacgcg
     4081 ccacaccaca cttatctgcc ctgactctct tttgcgggct ttttattaca ggaaagacac
     4141 cggacagtga aatgttaaat gcctcacaat tcaggtagtt gactgttgcc tgacatgctg
     4201 agcgtttgtt aaaaaaatcc tgcatgatga atccccctgg gcggcggggc ataatgacag
     4261 atgtttggtt gcgtattgta taggcaagtt gcggattctg tctggtcatt gcagaattca
     4321 ccgggaggca cccggcatca tgctgtatac agagattagg catatatcca ggctcctcat
     4381 cgcaggagcc tttttacatg caaaaaaagc ccgagtaggt tcgggcaaca gcatgagata
     4441 cttgcattgt catttttatc gtgtggattt taaccagggt ttataaggct gcgcaactgc
     4501 gcggcctttt tcgtattgcg ggctgttgtc tctcttctgc cattgtcctg taacttccgg
     4561 atttcagccc gctcctcatt ttactcacaa tattatcccg gccgggagga ttcatggcat
     4621 ttaaacacta cgatgtggtc agggcggcat cgccgtcaga ccttgctgaa cgactgacac
     4681 aaaaactgaa ggagggctgg cagccatttg gcagtccggt ggccatcacg ccttataccc
     4741 tgatgcaggc cattgcggcg gaaggtgatg tcaccacacc agtggcggtg accggtaatg
     4801 agagtaaggc ggtggctgtc agtgccacca gcgacccgga gtattacttt gttgtggttc
     4861 tggcagggca gtcaaatggc atgtcgtatg gtgaagg
//
LOCUS       DAZKHC020000195         4883 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZKHC020000195 DAZKHC020000000
VERSION     DAZKHC020000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.196"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            200..1153
                     /gene="ompT"
                     /locus_tag="ACHOSM_RS23425"
                     /old_locus_tag="pgaptmp_004685"
     CDS             200..1153
                     /gene="ompT"
                     /locus_tag="ACHOSM_RS23425"
                     /old_locus_tag="pgaptmp_004685"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HGU4561028.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIEAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1340..2824
                     /locus_tag="ACHOSM_RS23430"
                     /old_locus_tag="pgaptmp_004686"
     CDS             1340..2824
                     /locus_tag="ACHOSM_RS23430"
                     /old_locus_tag="pgaptmp_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4561029.1"
                     /translation="MRKIITHFKVILTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEADIQ"
     gene            complement(3008..3220)
                     /locus_tag="ACHOSM_RS23435"
                     /old_locus_tag="pgaptmp_004687"
                     /pseudo
     CDS             complement(3008..3220)
                     /locus_tag="ACHOSM_RS23435"
                     /old_locus_tag="pgaptmp_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            3370..4038
                     /locus_tag="ACHOSM_RS23440"
                     /old_locus_tag="pgaptmp_004688"
     CDS             3370..4038
                     /locus_tag="ACHOSM_RS23440"
                     /old_locus_tag="pgaptmp_004688"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4561030.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGASKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNTAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            4536..4718
                     /locus_tag="ACHOSM_RS23445"
                     /old_locus_tag="pgaptmp_004689"
     CDS             4536..4718
                     /locus_tag="ACHOSM_RS23445"
                     /old_locus_tag="pgaptmp_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGU4561031.1"
                     /translation="MTEHRGGAANFAEDRQKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQNSHGGGRKSDS"
     gene            4797..>4883
                     /locus_tag="ACHOSM_RS23450"
                     /old_locus_tag="pgaptmp_004690"
     CDS             4797..>4883
                     /locus_tag="ACHOSM_RS23450"
                     /old_locus_tag="pgaptmp_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HGU4561032.1"
                     /translation="MQIKSVEDIFIHLLSDTYSAEKQLTKALS"
BASE COUNT         1584 a         1014 c          938 g         1347 t
ORIGIN      
        1 agcaaaaaga aaatttccag catattaaac cacaacaata atacaacacc tggcaagttg
       61 cccacaaaga atgaataaaa caaaacaata attcaaactt aaacaaacta taaacagtta
      121 aacaatatac aattgactca ttaagttaga tatgaaaaat acatattcaa tcattaaaac
      181 gattgaatgg agacctttta tgcgggcgaa acttctggga atagtcctga caacccctat
      241 tgcgatcagc tcttttgctt ctaccgagac tttatcgttt actcctgaca acataaatgc
      301 ggacattagt cttggaactc tgagcggaaa aacaaaagag cgtgtttatc tagccgaaga
      361 aggaggccga aaagtcagtc aactcgactg gaaattcaat aacgctgcaa ttattaaagg
      421 tgcaattaat tgggatttga tgccccagat atctatcgag gctgctggct ggacaactct
      481 cggcagccga ggtggcaata tggtcgatca ggactggatg gattccagta accccggaac
      541 ctggacggat gaaagtagac accctgatac acaactcaat tatgccaacg aatttgatct
      601 gaatatcaaa ggctggctcc tcaacgaacc caattaccgc ctgggactca tggccggata
      661 tcaggaaagc cgttatagct ttacagccag aggtggttcc tatatctaca gttctgagga
      721 gggattcaga gatgatatcg gttccttccc gaatggagaa agagcaatcg gctacaaaca
      781 acgttttaaa atgccctaca ttggcttgac tggaagttat cgttatgaag attttgagct
      841 aggtggcaca tttaaataca gcggctgggt ggaagcatct gataacgatg agcactatga
      901 cccaggaaaa agaatcactt atcgcagtaa agtcaaagac caaaattact attctgttgc
      961 agtcaatgca ggttattacg taacacctaa cgcaaaagtt tatgttgaag gcgcatggaa
     1021 tcgggttacg aataaaaaag gtaatacttc actttatgat cacaatgata acacttcaga
     1081 ctacagcaaa aatggtgcag gcatagaaaa ctataacttc atcactactg ctggtcttaa
     1141 gtacaccttt taacgaagtt aaccagattt tctccccggt ggtattgtat aaccccgggg
     1201 aatttgttta cagttacaag taggcaatat ccactaattt tgtaagcatt taacctatgt
     1261 tataacgtgt aatctcttac cgcaacacaa gacctcaata tattcaaatt ttatctttat
     1321 tccgatattc agagacacaa tgcgaaaaat aattactcat ttcaaagtta ttttaacgtt
     1381 acttctacca gtaaccgtat ctgcccagca gatacagtgg caatcctgta tggccagtca
     1441 attcaaccac tggtttggtg aggaaaaacc gtctcctgac ttactatgtg ggtatttgtc
     1501 tgttccatta aaatatacag acacaggcgg agatgcttct tatgaaaaaa aatcacaagt
     1561 caaactagcg ttgacaaaat tgccggcaaa aagcaagcat aaaggaagta tcctgataat
     1621 aagtggtggt cccgggttac caggcataaa tccttatatt aactttgact ggccagtcac
     1681 aaatcttcgt gagtcatggg atattattgg atttgatcct cgaggcgtcg gacagtccac
     1741 accgacaata aactgccgac aatcagatac agagactcag gaaaacataa ccgaaaagca
     1801 acaagtatta aataaaatta atgcctgtat ccataatacc ggagccgaag tcattcgcca
     1861 tatcggctct aacgaggctg tatacgatat tgatcgtatt aggcaagcct tgggggataa
     1921 acaactgaca gccgtggcgt attcgtatgg aactcaaatt gcagccttat atgcagaacg
     1981 ttttccctac aacgtaagat ctatcgttct tgatggagtc gtcgatatcg atgacctgga
     2041 ggacaacttc acttggcaac tcaaacaggc acagagttat caggaaacgt ttgatcgctt
     2101 tgcatcctgg tgtgcgcgta caaaaagttg cccgctttct tcagacagag ataaggcaat
     2161 aactcagttc catgagctat tatcaaaatt acatcacaaa cctttattag acagtaaggg
     2221 agaaaatata tcttcagatg aactcatatc attaacaaca gaccttctgc tatggcgttc
     2281 atcatggcca acccttgcaa ctgccatacg ccagttctct caggggattg tcagtaatga
     2341 aattgaaact gcgctcagtg ctccgatagc ctcagaagag tcaagcgatg cttcgggggt
     2401 aatcctctgt gtagatcagg gggatgagca attaacacca gaagagcgaa aatcccgaaa
     2461 agacgctctt gcgaatgcct tcccggctat taactttgac aatggacgtt ccgattcacc
     2521 tgatttttgt gaattatggc caatacatag cgacctgaac aaaactcgcc tgaaaaatac
     2581 tgttctgccc tctggtttac tgtttgtagc acacaaatac gacccaacaa cgccctggat
     2641 taatgcccgt aagatggcag agaaattttc cagcccgtta ctaacaataa atggtgatgg
     2701 gcatacatta gctctcaccg gagttaattt atgtgtagat aaagcagttg tacatcacct
     2761 gatcactcca caaaaaatag aaaatatata ctgcccagga aattctgaag cagacataca
     2821 ataatttcaa aatattccgc tatttgctcc caatgcaaaa catatattgc attgggagca
     2881 tattcatatt tttgttattc ttgtatgaaa tcgtctcatg tacacggtcc ttttttcgcc
     2941 gaaaactgat attactttaa tcaaaagttg acgtgccaga ttcgaagtaa atttataaaa
     3001 tatcaaatca atatacattt tgcaccttcg agatagtaat ggctgttact gtaatatgag
     3061 ttacaaacag cttcatagcc agagctactg atgtatcaac acgattcaac aatacccgat
     3121 atttattcca tgcctccagc aacgatcttt cttcctccgt tgcgatttcc agatctacag
     3181 catcctgcag tggcgcaata tactcactga attcctggat gtagaactgt gtggtgacgg
     3241 tcttccagcc attcggctcc tgctgtatcg aagcatacca ggctatttca atatcgctat
     3301 gctgcggcag catttaaccc cttgtaattc atcgccataa ttgatttaat tcacaaataa
     3361 aactataaca tggtgaaatt aatgaaaaaa aacacagatg atggggctaa aatttacaca
     3421 ccacttaccc taaagcttta tgactggtgg gttttgggag tatcaaatcg gcttgcatgg
     3481 ggatgtccta caaaggaaca ccttcttcca cactttctgg aacatttagg taacaaccat
     3541 ctggatattg gtgttggaac tgggttttac cttactcacg tacctgagag tagtctgata
     3601 tctttaatgg atttgaacga agctagcctg aacgcggcat ctacaagggc tggggcatca
     3661 aaaattaaac ataaaattag ccatgatgtt tttgaacctt atcccgcggc gttacatggt
     3721 caatttgatt ccatttccat gttttacctt cttcactgcc tgcctggaaa tatatctaca
     3781 aaaagctgtg taatacgcaa tacggcgcag gccttaactg acgatggaac tctatacgga
     3841 gccacaattc ttggcgatgg agttgtgcac aatagcttcg gtcaaaaact gatgcgcatt
     3901 tacaatcaga aaggcatctt ttcaaacaca aaagattccg aagaaggctt aacacatata
     3961 ctctcagagc atttcgagaa tgttaaaacc aaggttcaag gtactgtagt aatgttttcc
     4021 gcttcaggga aaaaatagca tccaaccgca gcacgttctt gcatacgccg tgtctgcggt
     4081 ttaattttca ctctcaaata ttgtagttaa gtacaataat gattcctgtg gctatataga
     4141 tgtttttgta attagtcctt aagataaaat ttctctattt atccaagaaa cagacttcat
     4201 taggaagagt cgtaaaaaca ttaacaacta ccgccgtgta attaaattat tttttagaat
     4261 gttctgattg agaaaaacaa cgccatacac atcaaaagat atagaaatgt taatttagat
     4321 ttattaatct tttgagttac aacctaaact ttccgttaat caccaagata tcaatagatt
     4381 atactcattg agtacacatt tttacaattg ctatttttca cattaagatg cgcataccaa
     4441 tctcttattt gacactttct tatttgttac tagagttaat tgggtgctga caaggcacat
     4501 gttatttaca atttgaaaat cagaggggaa atgatatgac cgaacatcgt ggcggtgcag
     4561 ctaatttcgc tgaagatcga caaaaagctt ctgacgctgg tcgtaaaggc ggccaacata
     4621 gtggcggtaa ttttaaaaac gatccacaaa gagcatcaga agcagggaaa aaaggtggtc
     4681 agaatagcca tggtggcggc cgtaaatctg actcttaatt tatcaccccc tacattcgtg
     4741 aataactgaa gctaactgcg agcctacggg ctcgcagtga aaactctgga gtgattatgc
     4801 aaattaaatc tgttgaagat atcttcatcc acttattatc ggatacatac agcgctgaga
     4861 aacaactaac aaaggcactg tct
//
LOCUS       DAZKHC020000196         4795 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZKHC020000196 DAZKHC020000000
VERSION     DAZKHC020000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.197"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..224)
                     /locus_tag="ACHOSM_RS23455"
                     /old_locus_tag="pgaptmp_004691"
     CDS             complement(<1..224)
                     /locus_tag="ACHOSM_RS23455"
                     /old_locus_tag="pgaptmp_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU4561033.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLIEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQL"
     gene            complement(257..553)
                     /locus_tag="ACHOSM_RS23460"
                     /old_locus_tag="pgaptmp_004692"
     CDS             complement(257..553)
                     /locus_tag="ACHOSM_RS23460"
                     /old_locus_tag="pgaptmp_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HGU4561034.1"
                     /translation="MEQTSYSKLSQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPVAAGDSKQIDMQF"
     gene            complement(692..925)
                     /locus_tag="ACHOSM_RS23465"
                     /old_locus_tag="pgaptmp_004693"
     CDS             complement(692..925)
                     /locus_tag="ACHOSM_RS23465"
                     /old_locus_tag="pgaptmp_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4561035.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALAKTSVGYLDQIAYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            1039..1743
                     /locus_tag="ACHOSM_RS23470"
                     /old_locus_tag="pgaptmp_004694"
     CDS             1039..1743
                     /locus_tag="ACHOSM_RS23470"
                     /old_locus_tag="pgaptmp_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4561036.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAALGLPNGWLDLVHDVTPIASCSDSLTFVGQVRKGLVRVVG
                     EAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKVF
                     PGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILKQ
                     SRHLDDIEAREWLKSS"
     gene            1880..2167
                     /locus_tag="ACHOSM_RS23475"
                     /old_locus_tag="pgaptmp_004695"
     CDS             1880..2167
                     /locus_tag="ACHOSM_RS23475"
                     /old_locus_tag="pgaptmp_004695"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HGU4561037.1"
                     /translation="MKVLNVEKLHSFSRKHNQAKGALDSWYDEVIRENWKTTQDIRNR
                     FNSADFLPNNRVIFNIKGNNYRLVVQVVYQAGMVIVERVGTHAEYDKWRLK"
     gene            2164..2820
                     /locus_tag="ACHOSM_RS23480"
                     /old_locus_tag="pgaptmp_004696"
     CDS             2164..2820
                     /locus_tag="ACHOSM_RS23480"
                     /old_locus_tag="pgaptmp_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigA family
                     antitoxin"
                     /protein_id="HGU4561038.1"
                     /translation="MNRTSWRIIKNSEEHAAAMERLIELASSDLQPGTEDFDEFELLG
                     LLIEHYESREFPMDKPDPIEAIKFRMDQQGLSYADMKQYIGSASKVSEVLNRKRPLSL
                     SMIRRLHDGLGIPADILIQDMSAIEWSLVDAEEEETAMTSVIARCESAVTSPSAYFAE
                     KATESYFSKMLFSAVRGNGKCKEKRNVFSLISNLSSSFTAASNLNDEMTSDGNYLLLP
                     "
     gene            2817..3221
                     /locus_tag="ACHOSM_RS23485"
                     /old_locus_tag="pgaptmp_004697"
     CDS             2817..3221
                     /locus_tag="ACHOSM_RS23485"
                     /old_locus_tag="pgaptmp_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561039.1"
                     /translation="MKIELISKKVERLVMTRLEGDSTAKKAIKTTVNLNNELYTNVKD
                     SKLFRVRYFASVTIEGRLEMDITYDFDFRSEDDFSHEMAKSYEVRSIAPNMAYPYIKT
                     YAEQIILMSNLGRFTLPYFDFLANPMETNSNK"
     gene            4008..4076
                     /locus_tag="ACHOSM_RS23490"
                     /old_locus_tag="pgaptmp_004698"
                     /pseudo
     CDS             4008..4076
                     /locus_tag="ACHOSM_RS23490"
                     /old_locus_tag="pgaptmp_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            4135..4605
                     /locus_tag="ACHOSM_RS23495"
                     /old_locus_tag="pgaptmp_004699"
     CDS             4135..4605
                     /locus_tag="ACHOSM_RS23495"
                     /old_locus_tag="pgaptmp_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561040.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQRRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
BASE COUNT         1488 a          964 c         1046 g         1297 t
ORIGIN      
        1 agttgagaat cggtgattct gtccattggt ttattccacc cataggtttt acgcagaatg
       61 gcaagcagca ctttaaactg tcgcttggtc agatctgcgc ctgaataagc ctcaatcagc
      121 atatttgata gtctggcgta accatcatcg agatctgcca cattacgctc ctgttcggca
      181 aagttacctc tgccgaagtt gagtattttt gctgtatttg tcataatgac tcctgtggat
      241 tgatccagta attccctcag aattgcatat caatttgctt agagtccccg gcggccaccg
      301 gggatttttt ctttgtgatt tcatcaagcg catacttaaa agctctgcta atcggactga
      361 tgtctgatgc catgccaaaa gcacacaaga ccgaagctat aaacctccag tctgttctgc
      421 ttatcttcga ttcatgacag ccaatcatct ttgccagacc gcgctgggta agcgttgaca
      481 ggttgatgag taaatcagtt tcagcgcgat caatttctcg ctgtgatagt ttgctgtaac
      541 ttgtttgttc catttcttaa gatttccaat agtgaatagc tagttgaaag gtatgcgtgg
      601 aaacgcatat ggccttagtt ggtcagatat attgggactc gctttgtcag cgacgtagga
      661 cgaatgtcca ttgtgaaaat agcggtgtta cttatgcagc cgatgctcta cgcgatacga
      721 acactaggtt ttcctttttc acaggtttat aacccgtgaa attacgagta gcttcttcga
      781 ttgcattcgc tttatcaggg gaagctcttc gaaatccata tgcaatctgg tcaagatagc
      841 caactgaagt tttcgctaat gcggcgagtc gcttccattc ctcactagaa gcctcttttc
      901 gccagcgtag tagttcatta ctcattagtg cctccgttta tcacacagaa caactttacc
      961 attttgataa atcatccgca atgtaaattt atcatattgc gtatttatcc atttgctaaa
     1021 tagagggaaa attgtgagat ggaaaacaaa gatattcgca aatcgaatct ggcgtttttg
     1081 ctagatgagc ataaaaaaat cgcgggtaac actaatgcaa gctttgccga taagcttggg
     1141 gttagccctt ctcaactcac gcaagtctcc ggtgaaaaaa gcactcgaaa cataggggat
     1201 aaactagcaa gaaaatttga agccgcgctt gggttaccta atgggtggct tgatttggta
     1261 catgatgtaa caccaattgc atcatgctca gattctttaa cttttgtcgg tcaggtaaga
     1321 aaagggttag tgcgcgtggt tggtgaggca attcttggtg ttgatggtgc catcgagatg
     1381 accgaagagc gcgatgggtg gctcaaaatt tatagcgatg atccagatgc ctttggtctt
     1441 cgtgtgaaag gagacagcat gtggcccaga ataaaatcag gagaatatgt actcattgag
     1501 cctaacacca aagtattccc gggtgatgag gtgtttgtca gaactgttga aggacacaac
     1561 atgatcaaag ttcttggcta tgacagagac ggagaatacc aatttacaag catcaaccag
     1621 gaccacaggc caataacgtt gccttatcat caagtagcaa aggtggagta tgtggctggt
     1681 attctgaagc aatctcgcca tctggatgac atcgaggcaa gggagtggct gaaaagttcg
     1741 tgacttcatc gtcacatagc tggtaaccag tggcctgaag agacgtttgg gtgatgtaca
     1801 tagcatttct ggataaaaat acagattccc tttatgggaa atgaatctat aattcccaaa
     1861 gagggaacaa aatcggatta tgaaggtctt aaacgtagag aagcttcaca gttttagccg
     1921 gaagcacaat caggccaagg gggctttaga ctcttggtat gatgaagtga taagagaaaa
     1981 ctggaaaacg actcaagaca tacggaatag atttaattct gccgacttcc ttcctaacaa
     2041 cagggtaatt tttaatataa aaggcaataa ctatcggctc gttgtccaag ttgtttacca
     2101 ggcaggaatg gtcatagttg aaagagttgg aactcatgca gagtacgaca agtggaggct
     2161 taaatgaatc gaactagctg gcgcatcatt aaaaatagtg aagagcatgc tgcagctatg
     2221 gaaaggctca ttgaacttgc gtctagtgat ttacaacctg gaactgaaga ttttgatgag
     2281 tttgaactac taggcttgct tatcgagcac tatgagtcac gcgagttccc tatggacaag
     2341 ccagatccca tagaagcaat caagttccgt atggatcaac aaggcctctc ttatgccgat
     2401 atgaaacaat acattggctc agcatctaaa gtatctgagg tcttaaatcg taagcgtcca
     2461 ttaagtcttt caatgatccg tagactacat gacggacttg gaattcctgc agatatctta
     2521 attcaagata tgagcgcaat tgaatggagc ctagttgacg cagaggaaga agaaacagcc
     2581 atgactagcg tcattgctcg gtgtgagtca gccgtcacat caccttctgc ttatttcgct
     2641 gaaaaggcta cagaatctta cttttcaaaa atgttgttca gcgcagtaag gggtaatggc
     2701 aaatgcaaag aaaaacggaa tgttttttct ttgataagta acttgtcatc aagtttcaca
     2761 gcggctagca acctgaatga cgaaatgact tctgacggaa attacttatt attaccatga
     2821 aaattgaact cattagcaaa aaagttgaac gcttggttat gacacggcta gaaggcgatt
     2881 caacagcaaa aaaagccata aaaacaaccg ttaatctaaa taatgaactt tacactaatg
     2941 tgaaggattc aaagctattt agagtgagat attttgcctc cgtaactatt gaaggcaggc
     3001 ttgagatgga catcacatac gactttgact tccggtcaga agatgatttt tctcatgaaa
     3061 tggcaaaatc atatgaggta aggtccattg ctcccaatat ggcatatccc tatataaaaa
     3121 catatgccga gcaaattatc ctcatgtcaa accttggtag gttcactctg ccttattttg
     3181 atttcttggc caatcccatg gaaacgaaca gtaataagtg aaattcccca cccggcctca
     3241 gcgccgggtt ttctttgcct cacgatcccc acacctaaaa acacataacc aattgtattt
     3301 gttgaaaaat aaatagatac aacttgctaa acaacgcaat ccagatctcc ctcaaatctc
     3361 tttatttatc ctgtcgaatt cctacaacaa aataaaacac cataagaatc aatacgatat
     3421 ttgaaaacca agagaattta tcattttgct attgccatta atttatcatt ccgataaagt
     3481 tcacccatca gcaggacgca ctactcacca gggcggtgaa tatacaacga ttcgaatatg
     3541 aatctacggc gctgacaaag cgcaataacc aaagtgaact ttggggtgaa tgcagaagct
     3601 aaccttctcg gcggaggcgc tttgcaatga ttacgcgacc ggagttagtc gcccggctgt
     3661 attcaccgcc aaagttcatc aggaggttcc atatgacacg cagaactcag ttcaaaggca
     3721 attctcgttc tcgtcgtcgt gagcgtttaa aggcaaaggc attagctaac ggcgtactgg
     3781 cccgcgaaga agcaataagt tcagaagtat tacaccgccc aactctaagt agagcgcaga
     3841 ttcaggctaa aggtactcac gaaacgcctg atcgcataga agacgctaag ccaattaagt
     3901 tcatggcaca ggacgtgatc tggcaacaga aagaatacag acgcaatctg gagcgagcgg
     3961 ccattgtgta cgcgaatgag tttggacata agcaaccaga aactggtgta tgtcttccaa
     4021 acgtagccat ttacgcggca ggctaccgga aatcaaaaca actgacggcg aggtgacttg
     4081 tgttggtcgc cagaaaatga aattaggcag caaaccactt atttgaggtg agatatgaca
     4141 aaatcatgga gcgtaccttt tcctgaatca gaaactgaac atgatggaat gcctgttttc
     4201 tggagattcc aggcgacagt tgaagaagat gggataaaaa tattcgcact tcaatatata
     4261 gcttttcatc agacagagca ttatgcatgg ttggttcctg cgcattggat tgttaatttt
     4321 aaaccagcac caaatcagtg gttacaggaa tggaaacaaa ggagaaatag atatgcaatt
     4381 aagaaagtag caaaaaatgc agaaagatct tttgcattcc caacgaagaa acttgccatt
     4441 gaaagtttat tgcgccggaa gaaataccat ttaatgagaa tcaaacaaga tttggctgtt
     4501 gtatcaactc ttgttgatgg tatgaagaat attgatacat caacaccaga tattgaatat
     4561 aactttggac acaaccaaga aacagaaaat tgggtatttt attagtacga ataagcactg
     4621 tgtattcatt ccaacgagtg aatacacgga gcaatgtcgc tcgtaactaa acaggagccg
     4681 acttgttctg attattggaa atcttctttg ccctccaatg tgagggcgat tttttatctg
     4741 tgaggatatg aacagatgtc aaacatcaaa aaatacatca ttgattacga ctgga
//
LOCUS       DAZKHC020000197         4756 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZKHC020000197 DAZKHC020000000
VERSION     DAZKHC020000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.198"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            100..714
                     /gene="evgA"
                     /locus_tag="ACHOSM_RS23500"
                     /old_locus_tag="pgaptmp_004700"
     CDS             100..714
                     /gene="evgA"
                     /locus_tag="ACHOSM_RS23500"
                     /old_locus_tag="pgaptmp_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HGU4561041.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            719..4312
                     /gene="evgS"
                     /locus_tag="ACHOSM_RS23505"
                     /old_locus_tag="pgaptmp_004701"
     CDS             719..4312
                     /gene="evgS"
                     /locus_tag="ACHOSM_RS23505"
                     /old_locus_tag="pgaptmp_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HGU4561042.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEIEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4368..>4756)
                     /locus_tag="ACHOSM_RS23510"
                     /old_locus_tag="pgaptmp_004702"
     CDS             complement(4368..>4756)
                     /locus_tag="ACHOSM_RS23510"
                     /old_locus_tag="pgaptmp_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HGU4561043.1"
                     /translation="GNDKLFSALCQALELTELVNDPRFSSNILRVQNQAILKQYIERT
                     LKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQARNMLIEAGGIMMPGNPIKISG
                     CADPHVMPGAATLDQHGEQIRQEFSS"
BASE COUNT         1470 a         1013 c          950 g         1323 t
ORIGIN      
        1 gctcagattt tcttattttt attgagaaaa tgagatgacg ccttatgtct gtattactac
       61 agggagaagg gagatgcttc attgcaaagg gaataatcta tgaacgcaat aattattgat
      121 gaccatcctc ttgctatcgc agcaattcgt aatttattga tcaaaaacga tattgaaatc
      181 ttagcggagt tgactgaagg gggaagcgcc gttcagcggg tggaaacact taagcctgat
      241 atcgtcatca ttgatgtcga tatccccgga gttaacggta tccaggtgtt agaaacgctg
      301 aggaagcgcc aatatagcgg aattattatt attgtctccg ctaaaaatga ccatttttac
      361 gggaaacatt gtgctgatgc tggcgctaat ggattcgtga gtaaaaaaga aggcatgaac
      421 aatatcattg cggctattga agctgcaaaa aatggctact gctatttccc cttctctctc
      481 aaccggtttg ttggaagttt aacgtccgac cagcaaaaac tcgactcctt atcgaaacaa
      541 gaaattagtg tcatgcggta tattcttgat ggcaaggata ataatgacat tgctgaaaaa
      601 atgttcatca gcaacaaaac tgtcagcact tataaaagtc gcctgatgga aaaattagaa
      661 tgtaaatcac tgatggatct ttacacattc gcgcaacgta acaaaatcgg ctaatcacat
      721 gaagttttta ccctattttt ttcttctctg ttgtggtctt tggtcgacca taagtttcgc
      781 agacgaagat tacatcgaat atcgtggcat cagtagtaac aaccgtgtca cacttgatcc
      841 agtacgtctg agcaacaagg aattacgttg gttagcgagc aaaaaaaatc ttgtgattgc
      901 agtacacaag tcccaaacgg ctaccttgtt gcataccgat tcgcagcaac aggttcgtgg
      961 tattaatgct gattatttaa atcttttaaa aagagcgtta aatatcaaat taacactccg
     1021 ggaatacgca gatcatcaaa aagcaatgga cgcgcttgca gaaggtgaag tcgatatagt
     1081 gttatcacat ttagttactt cgccgcctct taataatgac attgctgcaa ccaaaccatt
     1141 gataattacc tttccggcgc tggtaaccac ccttcacgac tcaatgcgac cgcttacctc
     1201 accaaaacca gtaaatattg ctcgggtagc aaattacccc ccagacgagg taattcatca
     1261 atcatttcca aaagcaacaa ttatctcttt tacaaattta tatcaggcat tagcatccgt
     1321 ctcagctggg cacaatgatt actttattgg tagtaacatc attaccagca gtatgatttc
     1381 ccgctatttc actcactcct taaatgtagt gaaatattat aactcgccgc gtcaatataa
     1441 ttttttcttg accagaaaag aatctgtcat tcttaatgaa gtactcaata gatttgttga
     1501 tgctttaaca aatgaagttc gctatgaagt atcacaaaat tggcttgata caggaaacct
     1561 ggcctttctg aacaaaccat tagaactcac tgaacatgaa aaacagtgga ttaagcagca
     1621 tcccaattta aaggtgctgg aaaatcctta ctcgcccccc tattctatga cggatgaaaa
     1681 tggctcggtt cggggcgtta tgggggacat tcttaatatt attaccttgc aaacaggttt
     1741 aaatttttct ccgatcaccg tttcacacaa tatccatgct ggaacacagc ttagccccgg
     1801 aggatgggat ataatacctg gcgctattta tagtgaagat cgagaaaata atgttttatt
     1861 tgctgaagcc ttcataacaa cgccttacgt ttttgtcatg caaaaagcgc ctgacagtga
     1921 acaaacatta aaaaaaggaa tgaaagttgc cattccatat tattatgagc tgcattcgca
     1981 attaaaagag atgtatccgg aggttgaatg gatacaggtc gataatgcca gcgctgcatt
     2041 tcacaaggtt aaggaaggtg aacttgatgc tctggtcgcg acacagttaa attcgcgtta
     2101 catgatcgat cattactatc ctaatgaact ttatcatttt cttattcctg gcgttccgaa
     2161 tgcatcgctt tcgttcgctt ttcctcgcgg agaaccggaa cttaaggata ttattaataa
     2221 agcactgaat gcaattcccc caagcgaagt tctgcgcctg acggaaaaat ggattaaaat
     2281 gcccaatgtg accattgaca catgggacct atatagcgag caattttata ttgttacgac
     2341 attatccgtt ttattagttg gcagtagcct tttatgggga ttctacctgt tacgctcagt
     2401 tcgtcgtcgt aaagtcattc agggtgattt agaaaaccaa atatcattcc gaaaagcact
     2461 ctcggattcc ttaccgaatc caacttatgt tgtaaactgg caaggtaatg tcattagtca
     2521 taatagtgct tttgaacatt atttcactgc ggattactac aaaaatgcaa tgttaccatt
     2581 agaaaacagt gactcaccct ttaaagatgt tttttctaat gcgcatgaag tcacagcaga
     2641 aacgaaagaa aatcgaacaa tatacacaca ggtatttgaa attgataatg gcatcgagaa
     2701 aagatgcatt aatcactggc atacattatg caatcttcct gcaagtgaca atgcagtata
     2761 tatttgtggt tggcaagata ttactgaaac gcgtgatcta attaatgcac tcgagataga
     2821 aaaaaataaa gcgataaagg ctaccgtagc aaaaagtcag tttctggcaa cgatgagtca
     2881 cgaaataaga acaccaataa gctctattat gggcttcctg gaacttctgt cgggttctgg
     2941 tcttagcaag gagcaacggg tggaggcgat ttcacttgcc tacgccaccg gacaatcact
     3001 cctcggctta attggtgaaa tccttgatgt cgacaaaatt gaatcgggta actatcaact
     3061 tcaaccacaa tgggtcgata tccctacttt agtccagaac acttgtcact ctttcggtgc
     3121 gattgctgca agcaaatcga tcgcattaag ttgcagcagt acatttcctg atcattacct
     3181 ggttaagatc gaccctcagg cgtttaagca ggtcttatca aatttactga gcaatgctct
     3241 caaatttacc accgaggggg cagtaaaaat tacgacctcc ctggttcaca ttgatgacaa
     3301 ccacgctgtt atcaaaatga cgattatgga ttctggaagt ggattatcgc aggaagaaca
     3361 acaacaactc tttaaacgct acagccaaac aagtgcaggt cgccagcaaa caggatctgg
     3421 cttaggctta atgatctgca aagaattaat taaaaatatg cagggcgatt tgtcattaga
     3481 aagtcatcca ggcataggaa caacatttac gatcacaatc ccggtagaaa ttagccagca
     3541 agtggcgact gtcgaggcaa aagcagaaca ccccatcaca ctacctgaaa agttgagcat
     3601 attaatcgcg gatgatcatc cgaccaacag gctattactc aaacgccagc taaatctatt
     3661 aggatatgat gttgatgaag ccactgatgg tgtgcaagcg ctacacaaag tcagtatgca
     3721 acattatgat ctgcttatta ctgacgttaa tatgccgaat atggatggtt ttgagttgac
     3781 tcgcaaactc cgtgagcaaa attcttcctt acccatctgg gggcttacag ccaacgcaca
     3841 ggctaacgaa cgtgaaaaag ggttaagttg cggcatgaac ttatgtttgt tcaaaccgtt
     3901 gaccctggat gtactgaaaa cacatttaag tcagttacac caggttgcgc atattgcacc
     3961 tcagtatcgc caccttgata tcgaagccct gaaaaataat acggcgaacg atctacaact
     4021 gatgcaggag attctcatga ctttccagca tgaaacgcat aaagatctac ccgctgcgtt
     4081 tcaagcacta gaagctggcg ataacagaac tttccatcag tgtattcatc gcatccacgg
     4141 tgcggctaac atcctgaatt tgcaaaagtt gattaatatt agccatcagt tagaaataac
     4201 acctgtttca gatgacagta agcctgaaat tcttcagttg ctgaactctg taaaagaaca
     4261 cattgcagag ctggaccagg agattgctgt tttctgtcag aaaaatgact aaatagcggc
     4321 tcccacaatg ttcaaatgtg ggagctattt accagcacat cttacattta tgatgagaac
     4381 tcctggcgaa tttgttcccc atgctggtcg agcgttgccg ctcccggcat aacatgcggg
     4441 tccgcgcagc cgctgatttt tatcggatta cccggcatca ttattccccc ggcttcaatc
     4501 aacatatttc tcgcctgagt ttgtggcaat ttaatggcct cagccacact taataacggc
     4561 gcgacgggta caccaacttc atgtattctg gctaaccaaa cttcagctgc ctgcgttttt
     4621 aacgtccgct caatatattg tttaagaata gcctggtttt gtacgcgtaa aatattgctg
     4681 ctaaatcggg gatcattaac cagttccgta agctccagtg cctggcataa cgcagaaaaa
     4741 agcttgtcat taccac
//
LOCUS       DAZKHC020000198         4718 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZKHC020000198 DAZKHC020000000
VERSION     DAZKHC020000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.199"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..383)
                     /locus_tag="ACHOSM_RS23515"
                     /old_locus_tag="pgaptmp_004703"
     CDS             complement(<1..383)
                     /locus_tag="ACHOSM_RS23515"
                     /old_locus_tag="pgaptmp_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4561044.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSIT"
     gene            complement(380..958)
                     /locus_tag="ACHOSM_RS23520"
                     /old_locus_tag="pgaptmp_004704"
     CDS             complement(380..958)
                     /locus_tag="ACHOSM_RS23520"
                     /old_locus_tag="pgaptmp_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4561045.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(970..1323)
                     /locus_tag="ACHOSM_RS23525"
                     /old_locus_tag="pgaptmp_004705"
     CDS             complement(970..1323)
                     /locus_tag="ACHOSM_RS23525"
                     /old_locus_tag="pgaptmp_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU4561046.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(1335..1730)
                     /locus_tag="ACHOSM_RS23530"
                     /old_locus_tag="pgaptmp_004706"
     CDS             complement(1335..1730)
                     /locus_tag="ACHOSM_RS23530"
                     /old_locus_tag="pgaptmp_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4561047.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(1772..2797)
                     /locus_tag="ACHOSM_RS23535"
                     /old_locus_tag="pgaptmp_004707"
     CDS             complement(1772..2797)
                     /locus_tag="ACHOSM_RS23535"
                     /old_locus_tag="pgaptmp_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4561048.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVRDKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(2853..3185)
                     /locus_tag="ACHOSM_RS23540"
                     /old_locus_tag="pgaptmp_004708"
     CDS             complement(2853..3185)
                     /locus_tag="ACHOSM_RS23540"
                     /old_locus_tag="pgaptmp_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU4561049.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKASAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(3195..4514)
                     /locus_tag="ACHOSM_RS23545"
                     /old_locus_tag="pgaptmp_004709"
     CDS             complement(3195..4514)
                     /locus_tag="ACHOSM_RS23545"
                     /old_locus_tag="pgaptmp_004709"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4561050.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAM
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(4495..>4718)
                     /locus_tag="ACHOSM_RS23550"
                     /old_locus_tag="pgaptmp_004710"
     CDS             complement(4495..>4718)
                     /locus_tag="ACHOSM_RS23550"
                     /old_locus_tag="pgaptmp_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4561051.1"
                     /translation="VQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAG
                     LKPPAWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          963 a         1462 c         1250 g         1043 t
ORIGIN      
        1 gtaatggaat aagtcagatc tgcagaactc cataacgcca tatcgtcatc acgacgatac
       61 tcatagccct gcgtaaccat cgtggtaatc agtcctgcca gtgccgggat atcgctcatc
      121 accggataaa tccgggactc catccacgca tccagctctg aatccggcac ctgagcaggc
      181 aggaaaactt caatatgcag ctccgcctgc caggtatcgc tgtccagctc ttcgcccgtg
      241 tattcagcgc cggtgagata aacggcaact gccggaaaat ccgcctcatc aaaaacagcg
      301 gggcgaccat caaaaagcgt cgccccggtg tcatgcttct ccagtgcatc cagtacggct
      361 gcacggagtt cagtatgttt catcgcttta ttaccattct cagttgatgc tgcagcgcat
      421 agcccagctc tttcggaaga cgttcacgcc gtatccgttc aatattctgt ttaaacgccg
      481 tggtaagcgg caccgccatc gggattttca ccacatcaat ggggtaacgg tttttcccgg
      541 ccacacgctg catgacatgc caccgcccat ttttcagttg ctgaataaac gcgccgggaa
      601 tacgacggtt acccaccaca agcacgctgc cgccaccttt cagggatgaa cgctgcccct
      661 ttttacgacg cctgcgtcgg gacaggacaa cccgcgcatt ccccagcttg attacgggca
      721 aatccccccg gttaaccttg attctggcct gcggattttt aaccgtggcc cttttcagcc
      781 tggccctttc ctttaccagt ttccggcgta cctttgtctc acgggcaacc tgtgacgcag
      841 actgcgatat cgcggatgaa gcaacgcggt taatggccat tgctgacgca ccgggcaccg
      901 ccgttttgct gatacggctg aggttttcaa cggcctgctc aagacctttt atggccatac
      961 atcccccttt cagcggcgac ggttaacggc aggcggcacg ccacgcccaa gccagagatg
     1021 acagcttccg ccatcatccg gcgaaatccg gtctatccag aagttttcct caccgatggt
     1081 cagcgtgtct ccacgccgca gttgccgcac atcatcagtc cggacaaaca gggacgggct
     1141 ggagccttca acgcgcacgc cctgtccggc atagctgata ttttcagggt catcaaaaac
     1201 accacgtatt actgcgccgg actgctcacc ggatgtcatg gtggctgacg ttcccatgta
     1261 cccgcgtatc gtttcatcgg cgcgggcaat ggcagcatcg aacaggttat cgaaatcagc
     1321 cacagcgcct cccgttattg cattctggcc aggccacgtt ctgtcatttc ggctgccaca
     1381 ccggcagaga cacgaaacgc cgttcccggc agcacaaatg ccacaggttc atcccgcgtg
     1441 gcgtgaagtg catcggtatg cagcgtcacc agtgccacaa ccgtgaccag agcagccgta
     1501 tcagtcacgg tatccgtctg tgctgatacc acctcatttt catgtccggt cagcgcattt
     1561 tccgggctga cagacgtgtc ctgaccggct gcgtcatccg tgtcatcaag ctcctcttcc
     1621 agctctgcca cacggagcgc cagttcttct ttcgtccccg tcaggctgac atcacggttc
     1681 agttgttcac ccagcgagcg gagacgggca atcagttcat ctttcgtcat ggactcatcc
     1741 acagagaaac aatggccccg aagggccatg attacgccag ttgtacagac acgaactcat
     1801 cagggtcagc cagcagcatc agcggtgctg actgaatcat ggtgaactct cgcgccggat
     1861 cgccggtagt cacccagttt ttcgggtaac gggcagaggc gttaatgcct tcgcgctgtg
     1921 cgtccgcatc ctgaatacag ccataggtac gcaaaccgcg tgcctgagtg ttccccagca
     1981 ccatcgtgtt gtccggcagg aagttctttt tgacgccgtt ttccacgtac tgtccggaat
     2041 acacgacgat ggccacatcg ccatacattc ccttataaga caccgctttg cccaggtctt
     2101 ttaccgctgt ctccagctcg gaatgagagc cgcgacgggt atccagctta tccctgacgg
     2161 ctttgaagga acggaacagc gcccatcctt tcggatcaaa cacgatgata ttcaccacgc
     2221 cgctggcgtt cagcgcgtag gcttcgatat cgtcggtcgg gtcatacgtg gacttgtcac
     2281 gcttgctcca ctccgtgcca ccggactgtg tgatgttgtt tgccgcactg cggcccatat
     2341 ccacctcaac cggatcaaag gcttcaccgg tcatggtgta tttgccctta agcacggcag
     2401 aaactgcctg catctcttcg acctgggcaa tggccagctc ttcgtcacgc atgttctgca
     2461 tgatgatgcg acggcggcgg taagccgggt ccgccagatt ctgcggatct tcatccggca
     2521 ggcgacgcag ggtcatctgc ggattcacct catgcttcgg cttgacatat cccggcgtaa
     2581 attcagaggt ggagccgcca cgggaacgga taacctcacc ggaaacaatc ggcgaaacgt
     2641 acagcgccat gtttaccagt cccggaattt gtgagagata gactttctcc gtggtgaagg
     2701 gatagctctc acggaaaaag agacgcagaa acagcggatc aaacttaaat ttctgctcat
     2761 ttgccgccag cagctgggcg gttgtgtaca tcgacataaa aaaatcccgt aaaaaaagcc
     2821 gcacaggcgg cctttagtga tgaagggtaa ggttaaacga tgctgattgc cgttccggca
     2881 aacgcggtcc gttttttcgt ctcgtcgctg gcagcctccg gccagagcac atcctcataa
     2941 cggaacgtgc cggacttgta gaacgtcagc gtggtgctgg tctggtcagc agcaaccgca
     3001 agaatgccaa cggcagcacc gtcggtggtg ccatcccacg caaccagctt acgggtggcg
     3061 gtgtccagca tcagcggggt cattgcagac gctttcgcac tcaatccgcc gggcgcggtt
     3121 gcggtatgag ccgggtcact gttgcccagc ggctggtaat gggtaaaggt ttctttgctc
     3181 gtcataaaca tcccttacac tggtgtgttc agcaaatcgt taacggcatc agatgccggg
     3241 ttacctgcag ccagcggtgc cggtgccccc tgcatcagac gatccagcgc agtatcactg
     3301 cgcgcctgtg cactctgtgg tgctgcggcc agaatgcggc gggccgtttc cacggtcata
     3361 ccgggggttt ctgccagcac gcgggcctgt tcttcgcgtc cgtgagcctc ctcacagttg
     3421 aggatcccca taatgcggct gttttctgcc gcaaccgcag cggtgatctg cgcgttcacg
     3481 tccggctgcg ccgcgctggc gttttcgccc tccatcgctg gcaccacgcc agtaacgtca
     3541 gcctgcgaag cagtggctga aacagttgtt gattgagtct ctttggtcat tcgccctcct
     3601 gagagacggg atttacgtgc atccagtgca tcacgcataa cggtgatcgc atcggtgctg
     3661 ttgacaagtt catcagccag tccggcatca atggcctcct gaccgctgta cactgcagcc
     3721 tcggtatcca gcacagcctg cacggacagg ccggtatatg ccgacacctt ctgcgcaaac
     3781 atccggcggg ttgcatccat ccgggactgc agtgtttccc ggacatcacc cggtagatgg
     3841 ctgtaggggt tgccatccac cttatggctg ccgctgtaaa tcagcgtgat ttccacgccc
     3901 tgtttctcca gggcagcacc gtaattactg tgagccatca tgacgccgat ggagcctgtc
     3961 cgggcggtct gcgtgaccag acgccgggag gcggcgctgg caagcagctg acctgcactg
     4021 cagttcatgt cgttggccag cgcccatacc ggttttatgt cacgcacacg ggcgatgatg
     4081 tcagcacagt caaatgctcc cgccaccatt ccgcccggtg tgtccatatc gagcagaatg
     4141 ccgtccacca tcgggtcgct ggcagcctgt tgcagacggg cgataatgcc gttgtaaccg
     4201 gtcatccccg aatacggctg cagcgcccgc gtccggctga ccagcgtacc ggacaccggc
     4261 agcacggcga tgccgttcat gacctgataa ctccgggcct gtcgtggtcc gtcatcatca
     4321 ccggataacg ccagcgccgc gggtgcctct ccggcagtca ggctgtcgcc ggatactgca
     4381 tccgtcaggc ggctgatccc aagctggcct gcaagcgcac aaaagaaaac ccgcgcatag
     4441 gcgggttcaa gcatcagcgg ctcattaaaa gccatgctgg caatatgcgg gagattacgc
     4501 agctctgctg tcactcttct cctcctctgt tgattgtcgc agcccggatt caaatgccgc
     4561 agccgcccag gcgggcggtt taagaccagc tgcgcggcgc tccatcgttt cacggacctg
     4621 ctgggcaaaa atttcctgat agtcgtcacc gcgtttcgcg cactctttct cgtaggtgct
     4681 cagtccggct tctatcagca tcaccgcttc ctgaactt
//
LOCUS       DAZKHC020000199         4707 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZKHC020000199 DAZKHC020000000
VERSION     DAZKHC020000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.200"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(45..620)
                     /locus_tag="ACHOSM_RS23555"
                     /old_locus_tag="pgaptmp_004711"
     CDS             complement(45..620)
                     /locus_tag="ACHOSM_RS23555"
                     /old_locus_tag="pgaptmp_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU4561052.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(689..1267)
                     /gene="terD"
                     /locus_tag="ACHOSM_RS23560"
                     /old_locus_tag="pgaptmp_004712"
     CDS             complement(689..1267)
                     /gene="terD"
                     /locus_tag="ACHOSM_RS23560"
                     /old_locus_tag="pgaptmp_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HGU4561053.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(1316..2356)
                     /gene="terC"
                     /locus_tag="ACHOSM_RS23565"
                     /old_locus_tag="pgaptmp_004713"
     CDS             complement(1316..2356)
                     /gene="terC"
                     /locus_tag="ACHOSM_RS23565"
                     /old_locus_tag="pgaptmp_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HGU4561054.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(2379..2834)
                     /locus_tag="ACHOSM_RS23570"
                     /old_locus_tag="pgaptmp_004714"
     CDS             complement(2379..2834)
                     /locus_tag="ACHOSM_RS23570"
                     /old_locus_tag="pgaptmp_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGU4561055.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(2857..4014)
                     /locus_tag="ACHOSM_RS23575"
                     /old_locus_tag="pgaptmp_004715"
     CDS             complement(2857..4014)
                     /locus_tag="ACHOSM_RS23575"
                     /old_locus_tag="pgaptmp_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU4561056.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(4014..4595)
                     /locus_tag="ACHOSM_RS23580"
                     /old_locus_tag="pgaptmp_004716"
     CDS             complement(4014..4595)
                     /locus_tag="ACHOSM_RS23580"
                     /old_locus_tag="pgaptmp_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HGU4561057.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         1182 a         1271 c         1144 g         1110 t
ORIGIN      
        1 cccaaaaaaa accccggcat tgccggggtc agactcagcg tcttttagac attgacgccg
       61 tgctgggaag caagcgctga cagaccaccg gcaaaaccct ggccaacagc tttaaacttc
      121 cactcagtac catggcgata cagttcaccg aagaccattg cggtttcggt tgaggcgtct
      181 tcagacagat cgaaacgggc aatttccgtc ccgttgtcgt tgttgtaaac gcgcatgaag
      241 ctgttgctca ccatgccgaa gttttgttta cgcgcttctg catcatagat ggtaacggca
      301 aataccagtt ttttgatgtc tgctgagact ttggtcagat cgattttgac ctgctcatcg
      361 tcgccgtcgc cttcaccggt acggttgtcg ccctggtgct ctactgcgcc atcagggctg
      421 gttttattat tgaagaaaat gaaatgggca tctgacagta ctttaccgtc ttcacctact
      481 gcgaatacgg aagcgtccag atcaaaaccc tgaccatcgg ttacacgggc atcccagccc
      541 aggccaacca tagcgacatt catggttggt gcttctttgg tcagagatac gttgccgcct
      601 tttacgagag aaactgccat ttttagctcc tgcaaacagt tgaatgaggc tgaattaaca
      661 gccccagata tgaaaagtta cttttcgatc aggacgcgtt aatgccgtac tgagcacata
      721 cagatgccag accaccagca taaccctggc ctactgcgcg gaatttccac tcaccattgt
      781 ggcgatacag ctcgccgaac agcatggcag tctcagtgga cgcatcttcg gtcagatcgt
      841 agcgagcgac ttcagtctgg ttatcgtcat taaccagacg aataaacgca ccggatacct
      901 gaccaaagct ctggcgacga gcctgagcat cgtggatggt cacaacgaag atgatcttgt
      961 caacttcaga cgggacggcg tccagtttaa ttttcagcga ttcatcatca ccatcgccct
     1021 caccggtgcg gttatcgccg gtgtgcgtta cggaaccgtc ggatgacgtc aggttgttat
     1081 agaagatgaa atctgaatcg ccgcgcactt tgccgtttga ggccagcagg aatgctgaag
     1141 catccaggtc aaagtcctga ccgtctgttg aacgcgcatc ccagccaagg cccaccagga
     1201 catttttcat tgacggagct gctttactca gggagacgtt cccgcctttg gaaagagaaa
     1261 cactcataaa ataacctctt cgattagtaa ttgttcaggt taacacttaa ggggattaac
     1321 tccccttttc ctcagattca ggtttgcccg ggaacatgac gcttgcgaga atgcccagcg
     1381 ccagtacacc cagtacaaca tacaggctgg ttgttgccgc gatgctgtaa ccatgatgcc
     1441 agatgtgatc ggtcgcattc aggccgagtt ttgccgcgat gaagaacagc agcacgataa
     1501 cggccttctc cagatgaacc aggtactgtt tcagtgcctc aaggacaaaa tacagagtac
     1561 gcaggcccag gatagcaaac atcatggcac tatagacgat aagcggttca cgactgacgg
     1621 caatgattgc cggtaccgag tcaaacgcga acattacgtc cgagagttca accacagcca
     1681 cacacaggaa cagcggggtc gcatacagcg ccgctttttt accacggcca atggtgacat
     1741 cgctgttttc tggtttcgcc agttcagcat ccacttcctt ctggttaagt aggaaggcat
     1801 gccctctgag cttcggccag atagggaaga agcgtttaac catacggtaa gccagatgct
     1861 gggagtaatc ctcaatttca tcatcgtcat caccgctttt aagcatcatg accgccgtcc
     1921 aggcaacgat aatagcgaag acaacttcaa cgtacggccc cagactcagc aggctcgtac
     1981 cgatggcgac aaagatgccc ctgaagacga tggcaccaat gatcccccag tagagaacac
     2041 ggtggcgata acgatccgga acggcgaacc aggagaagat tgccatcatg acgaacaggt
     2101 tatcgaccga cagcactttc tccagcgcat aacccgtgac aaacagactg gcaacctcag
     2161 caccgtggtg gatatagagg aaaccggcaa atgccatcgc aaccacaacc cagaatacgg
     2221 accagagcgc agcactcttc agcgagattg gcttgtcatc acggtgcata aacaggtcaa
     2281 taaagatggc accgactgaa agcgcaataa aaacaatgac cgtttcagtc gggaagccga
     2341 tgtgtgtgga aaccatattt tacccttagg gatgtggatc agaggccaaa ttcagccggg
     2401 tcgaagccca acgcgatagc gatctcgcgg gaggccagtt tctcgtcctg atcgaagtta
     2461 ccgtcacttt tcgcaacggc aataccaaca cgtaaggcca gctgagcggc ctcaggctga
     2521 tctttcagcg ccaggatgta tttcatggtt tcgcccttgc caatttcaac atcgaaatcg
     2581 aagcttgaaa ccagtttatt gaagaactca atcacctcat tggtatcgaa gaccttcagc
     2641 tcttctgaag agcgcagaaa gcccatcatt ttctgctttt cttccgaact tacgccgtca
     2701 ctcgatacgg caatacgggc acatacagca acggtgccct gcatgaattt tttgtttttg
     2761 aaacggccaa cctggcgggt cagttcgtca cggcctgagt taatggcacc tttaactttg
     2821 tcgaaaaagc tcatacctgt ttcctttgtt gctgtgttat ttagaaccgg cgctccagcg
     2881 aaatccccag ccaaatgccc ggtccatttc gtcctggcct ttgaagtact ggttaattcg
     2941 ctcgacttta attgcgccgt tttcgtttac gagtctggca atggcgcaca atgtgcgacg
     3001 gttctcacct tcggtcaggc gggtctcaat gggcggttga tccggcacat gaatggtgac
     3061 tacaccatca gtcttgtccc agctgggaac accttcataa ataaaggcgt aaatcagcac
     3121 ttcgcggata tgcttccatt cacgtccatt gatatgcagc cactcgccgt ctgatacatc
     3181 acccgtccgg tcatcgccct tgagctgaac atacggttca tcgcgataat caccgaatgc
     3241 attaccgagc gcctgaatga ccgatttata cccgtcctga agctcaacaa aggcccccag
     3301 atccaggtcg attcctttgg agccgaacat acctgcaaag cctgatttgc tactgccccg
     3361 gtgccagttc aggttgatcc ggatttcacc gaagttatcc cgtttggtca ggctgattgc
     3421 aggcttctct tttgtcagcg ataccttgct caggctgatt tttgtctcaa ctggcggagg
     3481 ggttacaacc ggaggagttg gggctgcggg agccggttcg tcggcaatat ttacgccgaa
     3541 atgctctgcc agcggtttaa gtccaccgtt gaacccctgc gcaacgaagc ggaatttcca
     3601 gtcattatta cgacggtaaa attcccccag aattaaggct gcttcctgac ggccgctcag
     3661 ttcgacactg ccgctaacca ggccggtagc cccttgctcc acgtcgatgg aaagattacg
     3721 aagaccggac accgtctgcc caccgtcaca ggtcacggtg aacgcaattt tctggacatc
     3781 aggtttcagc cggttgagcg cgacagtaaa ggttgagtac tggccttcac tgaccaggct
     3841 gacggtacca tcatcgttac gtggctgacc ataaaacacc atgtccgcat caccctgcac
     3901 cttcccgtcg gcatacaggc gaaacgccga agcatccacc tggccgcccg aggtaatccg
     3961 gacccgcaac tcctgggctg gaacgggggc attgcccccc ggcgtcaggt tcattagcga
     4021 ataaccgctg aaacgatatc ggagtgcata tcgtcaattg tgcggccgcg acaggcatgg
     4081 ccaagggcgg taaagtccca gttgccgtta ttacggcgca gcgaagaaat aacgatcccg
     4141 gtatgtgagc cctgctcagt cagcttgtag cgagctagct ctttgccggt ttgatcgaca
     4201 acgcggcaga aagcgttttc gacgtcattg aacgactgac cacggaagct gtttaccgta
     4261 aacgccaggt attcaacgtt tgcaggcagg cgggagaggt tgaccttaat cacttcatcg
     4321 tcaccgtcac cctcaccggt caggttatcg ccactgtgca caacagcgcc gcaggtggat
     4381 ttcagtttgc ggaaccagat ggtgtcaatt gttttacctg tgctgtccat taatacacag
     4441 ccagcatcga gatcgattga gtcgttgcct ccaaacaatc ctccaagaag accttttttc
     4501 ttaaccggat cccaaccgag accgaaatga agctggctta atgcagatga ttctttgctg
     4561 agtgataccg tctggttctt aactaatgaa accatgtttt attccttaaa atggtaatga
     4621 ccgagacaat caaaggtata tttagtaact gacgaagaaa atcatatcat gatgaagttc
     4681 tctgacaagg gaaatttttg tggtttt
//
LOCUS       DAZKHC020000200         4668 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZKHC020000200 DAZKHC020000000
VERSION     DAZKHC020000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.201"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..386
                     /gene="grlA"
                     /locus_tag="ACHOSM_RS23585"
                     /old_locus_tag="pgaptmp_004717"
     CDS             <1..386
                     /gene="grlA"
                     /locus_tag="ACHOSM_RS23585"
                     /old_locus_tag="pgaptmp_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HGU4561058.1"
                     /translation="VIPDSVKNYDGEPLYILVSLWCKLQEKWISRNDIAEAFGINLRR
                     ASFIITYISRRKEKISFRVRYVSYGNLHYKRLEIFIYDVNLEAVPIESPVSTGPKRKN
                     YRVGNGIVGQSNIWNEMIMRRKKEN"
     gene            complement(772..1227)
                     /gene="cesD"
                     /locus_tag="ACHOSM_RS23590"
                     /old_locus_tag="pgaptmp_004718"
     CDS             complement(772..1227)
                     /gene="cesD"
                     /locus_tag="ACHOSM_RS23590"
                     /old_locus_tag="pgaptmp_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HGU4561059.1"
                     /translation="MSRKFSSLQDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNRKMAKKAFKACLMWCNEQEKYATYKENIKKLLGNTD"
     gene            complement(1241..2779)
                     /gene="escC"
                     /locus_tag="ACHOSM_RS23595"
                     /old_locus_tag="pgaptmp_004719"
     CDS             complement(1241..2779)
                     /gene="escC"
                     /locus_tag="ACHOSM_RS23595"
                     /old_locus_tag="pgaptmp_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HGU4561060.1"
                     /translation="MKKISFFIFTALFCCSAQAAPSSLEKRLGKNEYFIITKSSPVRA
                     ILNDFAANYSIPVFISNSVNDDFSGEIKNEKPMKVLEKLSRLYHLTWYYDENILYIYK
                     TNEISRSIITPTYLDIDSLLKYLRDTISVNKNSCNVRKITTFNSIEVRGVPECIKYIT
                     SLSESLDKEAQSKAKNKDVVRVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNG
                     SLPSTGKGAVERNGNLFDNSVKISADPRLNAVVVKDREITMDIYRQLISELDIEQRQI
                     EISVSIIDVDANDLQQLGVNWSGTLNAGQGSIAFNSSTAQANLSSSVISNANNFMIRV
                     NALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTP
                     RILDDNSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSILPEVQNSEMTTEATLS
                     AGESLLLGGFIQDKESSSKDGIPLLSDIPVIGNLFSSTTKQKHSVVRLFLIKATPIKS
                     ASSE"
     gene            complement(2779..3234)
                     /gene="sepD"
                     /locus_tag="ACHOSM_RS23600"
                     /old_locus_tag="pgaptmp_004720"
     CDS             complement(2779..3234)
                     /gene="sepD"
                     /locus_tag="ACHOSM_RS23600"
                     /old_locus_tag="pgaptmp_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HGU4561061.1"
                     /translation="MNNNNGIVKNDSDWLTALDFVKDVKGSPKHLTFHIYQKNAFLHD
                     FGSYWVLYIELNGDFRQVPTDTFIRLCNILAVSKEYKQMGIFQSNKKWYLCQLFNKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQASTSDTTMPPTHLFSDIGRIV"
     gene            complement(3240..3812)
                     /gene="escJ"
                     /locus_tag="ACHOSM_RS23605"
                     /old_locus_tag="pgaptmp_004721"
     CDS             complement(3240..3812)
                     /gene="escJ"
                     /locus_tag="ACHOSM_RS23605"
                     /old_locus_tag="pgaptmp_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HGU4561062.1"
                     /translation="MRKYIKNTFLFVFICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNVTISVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            complement(3814..4191)
                     /gene="sctI"
                     /locus_tag="ACHOSM_RS23610"
                     /old_locus_tag="pgaptmp_004722"
     CDS             complement(3814..4191)
                     /gene="sctI"
                     /locus_tag="ACHOSM_RS23610"
                     /old_locus_tag="pgaptmp_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HGU4561063.1"
                     /translation="MNNINQSENINIHFNKVSQTNFVDDHTPLAPTPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            complement(4275..4577)
                     /locus_tag="ACHOSM_RS23615"
                     /old_locus_tag="pgaptmp_004723"
     CDS             complement(4275..4577)
                     /locus_tag="ACHOSM_RS23615"
                     /old_locus_tag="pgaptmp_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SepZ"
                     /protein_id="HGU4561064.1"
                     /translation="MDAANLSPSGGVLPLAATINGNVSVDENTVVMTPENGVSRNIRI
                     VAGIALATTALAALGTGIAMACTTNASSQEFLGLGIATGVLGGVTALGGGLAMKYA"
BASE COUNT         1353 a          863 c          884 g         1568 t
ORIGIN      
        1 atgtaattcc tgactcagta aagaattacg atggtgagcc tctgtatatc ttggtttctc
       61 tttggtgtaa attgcaggag aaatggattt ctcgcaatga tattgccgaa gcattcggta
      121 taaacctgag gagagcatca tttattataa cttatatatc gagaagaaaa gaaaaaattt
      181 catttcgtgt cagatatgtt agttatggta acttgcatta taagcgcctt gagattttca
      241 tttatgatgt taacctcgag gctgttccga tagaaagtcc ggtatcaacc ggaccaaaaa
      301 gaaaaaacta ccgagtcggt aatggtattg tggggcagtc taatatctgg aacgaaatga
      361 tcatgaggcg gaaaaaggag aattaaacgt atttttttga gtatatttta tagaagaata
      421 aaaataaagc tctattcaag ggctttgttt tccaggataa gcctttttct ctgccaaagt
      481 ttgaaagtca tttcctgaca aatacactct gggctataga tgattgaaac agtcagattt
      541 ctgatgctaa gttaagatat atactgctta ttctataagc ggaaatgaca ttgagtacag
      601 cagaagatgg ttcaggcgca taaaatcctg gttaatggag taacccactc ctgtagataa
      661 atctgccagc ggtcggtgct caacgttggg cgcacttcac caaagcagct ttattatttc
      721 cgatagaaaa tccccagaca gcctggggat tggtatggca gatggtagtt tttaatctgt
      781 attacctaac aattttttaa tattttcttt atatgtagcg tatttttctt gttcattaca
      841 ccacattaaa caagccttaa aggctttttt ggccattttc cgatttccga ccaataagta
      901 gctaatccca gaataataag atgccctggg atcttgcata actaaagttg cacagcgagc
      961 gaaacacagt tgtgcatctt catgatttga taaacgctga tgacacaacc ccagagatag
     1021 ggtgtagtca taattccagt gctccagata tgttagcaga tgaaatagat ttcttgcggt
     1081 tataacatca ccagattgat atgctgtata ggcataagaa tgaagatcat tgagatcttg
     1141 ttgcgtcaaa tttgtcagtg atgctaacgt accaccatcc tggtagaaat catagatatc
     1201 ctgtagagag ctaaatttcc tgctcataaa gcctcattta ttattcgcta gatgctgatt
     1261 ttatcggggt cgctttaatc aaaaagagtc gaacaacgct gtgtttttgt tttgttgtac
     1321 tggagaataa gttaccaata acaggaatat cacttaacaa cggtatacca tctttacttg
     1381 aactctcttt atcctggata aatccgccaa gcagcagact ttctccagcg cttaatgtcg
     1441 cttctgttgt catttctgaa ttttgtactt caggtaagat gctgctggca tcctgagcat
     1501 tactttggtt agtcgattga ttgccatcct gaatatcaag taacaaacga actcgttccc
     1561 gacgttttcc ggtaagacta ttcgagttgt catcaagtat acgtggtgtc actctcagta
     1621 atgttccgct ggtaatactt tccagagatg ccaccttttc tccggaaact tttgtataaa
     1681 acgttacgtt tttatcaaga atcgcctgca tattatttaa ggttataatt gatggctgag
     1741 agagtatttt tgctttcgag ttctgttgta acgcattcac tctgatcatg aaattattgg
     1801 cgttgcttat cacagaagaa ctgagattag cttgcgccgt tgagctgtta aaggcaatag
     1861 aaccttgccc tgcatttaat gtaccggacc agtttactcc cagttgctga agatcatttg
     1921 catcaacatc aataattgaa acagaaatct caatttgcct ttgctcaata tcaagctcag
     1981 aaattaattg ccgatatatg tccatcgtta tttcacgatc ttttacaaca actgcattaa
     2041 gacgtggatc tgcggaaatc tttacactgt tatcaaacag gttgccgttt ctttctacag
     2101 cacctttgcc tgtgctggga agtgagccat tacttgccat cgtcttcagg atggagacga
     2161 cgccaggcac gacaacattt tggtcgcggt atttatacgt tatatctgtc gctgatgcat
     2221 agtttaattt aaatactcta acgacatctt tattttttgc tttagactgt gcttctttat
     2281 caagactttc agataaagat gtaatgtatt ttatgcattc aggaacgcca cgaacttcaa
     2341 tcgagttaaa tgttgtgatt tttctcacgt tacaggagtt tttatttact gagattgtat
     2401 cacgcagata ttttaacagt gagtcaatat ccagatatgt tggcgtaatt atgcttcttg
     2461 atatttcatt tgttttataa atatacaaaa tgttctcatc ataataccat gtgagatgat
     2521 agagcctgga cagtttttcc agtactttca ttggtttttc attttttatt tcaccagaga
     2581 aatcatcatt cacagaatta ctgatgaaaa cagggatgct ataattagcg gcaaaatcat
     2641 tgaggattgc acgtaccgga gagctttttg taataataaa atactcgttc ttgcctaatc
     2701 ttttttccag agaggacggg gcagcctgag cactgcagca aaatagtgct gtaaaaataa
     2761 aaaaacttat ttttttcatt acacaattcg tcctatatca gaaaacaaat gtgttggagg
     2821 catcgttgta tctgatgtag atgcttgctc taacttatca aattgcttgt caagcaaaga
     2881 ggcgagtgtg tgttgcatta ttgcttttga catgtttgcg cgatgattat tatctttatt
     2941 aaacagttgg caaagatacc attttttatt agattgaaaa atgcccattt gtttatattc
     3001 tttactaacg gccagaatat tacagagtct gatgaaggta tctgttggaa cctggcgaaa
     3061 atcgccatta agctcgatat acagaaccca gtaacttcca aagtcgtgta agaaggcatt
     3121 tttttgatag atatgaaaag taagatgctt tggtgaacct ttgacatctt ttacaaaatc
     3181 aagagccgtt agccagtcac tatcattctt aacaatgcca ttattattgt tcatacatat
     3241 tacccgtcct gtcctgagga tgacttgata acgacactaa tattttctag tttgagatcg
     3301 tctacgctat tcttaactaa gttttttatc tgaatgactg atggtgctaa gttaacctct
     3361 ggcgagctaa tgactaaaac agccgctgag gatggttggc tctcgttatt gttaacattt
     3421 aatgaaacgc tgcaatcgat aacaccgggg attttactta gtaacctttc tatatcttgt
     3481 tctttgagat agttgatttt tgcattttct tgtgatggcg atgccactag ctgactgggc
     3541 ggaaaaataa cttcaatatc tgcaaatttc tttttcggga agccattgtt atttaaaatg
     3601 gttatcgcac ggacaaaatc ctctttttcc acacttattg ttacatttcc acttttgtcc
     3661 atttctttag atacattgac atcattggat aacaacaatg cttgcatctg attggcttct
     3721 ttttccgtta atcctgtata caattgctct ttacatgcaa ctgtcaaaca aataaaaaca
     3781 aataaaaagg tgtttttaat gtattttctc attttattgc attgaaacca gttttgtaac
     3841 cgatgttgaa agaagacttg ctaacttgga tacgacatca acattgacgt ttgcgttgag
     3901 cacattcttt tgtaatgtga gcacatcttc tggcgagagt ccacctgcag atagggcatc
     3961 gaaactgaga tcggtgttca ttgttgcaag atgtctcttt ttaatttctt caattaaaac
     4021 ttgctctgga gaagatacac cagctgtttt aggaagtaat tgatcaagaa actgagccgc
     4081 tccagccgca gagggcgttg gtgctaacgg ggtatggtca tcaacgaaat ttgtttggga
     4141 tactttatta aaatgaatat taatattttc tgattgatta atattgttca tatttacggt
     4201 tagtctcttt tcatgagaga ggtaatggta taatgcatcc atgacctctc tattttattt
     4261 atgcgatgcg atgattaggc atatttcatc gctaatccgc cgccaagcgc agtaacacca
     4321 cctaatacac cggtagcaat acctaaacct aagaattctt gggagcttgc atttgttgtg
     4381 catgccattg cgataccagt acctaaagca gccagagctg tagtagcgag tgcaattccg
     4441 gctacaattc tgatattacg acttacccca ttttctggtg tcattactac tgtattttca
     4501 tccaccgaaa catttccatt gattgtggct gccagtggta atactccgcc agaaggactt
     4561 aaatttgctg catccattaa cttttctcca ttgtctaatt tagacatttg tctggttatc
     4621 aataaccaaa ttatcccatg ggcaactatt atattattta aatagttg
//
LOCUS       DAZKHC020000201         4593 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZKHC020000201 DAZKHC020000000
VERSION     DAZKHC020000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.202"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..419)
                     /locus_tag="ACHOSM_RS23620"
                     /old_locus_tag="pgaptmp_004724"
     CDS             complement(<1..419)
                     /locus_tag="ACHOSM_RS23620"
                     /old_locus_tag="pgaptmp_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HGU4561065.1"
                     /translation="MARLLTIVFFFSISASAVTARQFQRELDLADKAILWALISASTK
                     EGRKACSLSYFSCKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLL
                     SKGKIIRPYLKNLNPLQLAADCIETVNKIKDKNKKSL"
     gene            complement(1043..1504)
                     /locus_tag="ACHOSM_RS23625"
                     /old_locus_tag="pgaptmp_004725"
     CDS             complement(1043..1504)
                     /locus_tag="ACHOSM_RS23625"
                     /old_locus_tag="pgaptmp_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGU4561066.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            complement(1516..>4593)
                     /locus_tag="ACHOSM_RS23630"
                     /old_locus_tag="pgaptmp_004726"
     CDS             complement(1516..>4593)
                     /locus_tag="ACHOSM_RS23630"
                     /old_locus_tag="pgaptmp_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4561067.1"
                     /translation="DRITITDSLNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAV
                     GRLRTQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLEMR
                     RKYDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLTVTD
                     CSGYVTRYDHDRFGQVTAVHREEGLSQYRAYDSRGQLIAVKDTQGHEMRYEYNAAGDL
                     TAVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDV
                     LDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVNGETAEQW
                     QYDKRGWLTDISHLSEGHRVTVHYRYDEKGRLTGERQTVHHPQPEALLWQHETRHAYN
                     AQGLANRCIPDSLPAVEWLAYGSGYLAGMKLGDTPLVDFTRDRLHRETLRRFGRYELT
                     TAYTPAGQLQSQHLNSLQYDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHTIA
                     ANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDRYGRLTEKTD
                     LIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRR
                     ERDLTGWMSLSRKPEVTWYGWDGDRLTTIQNDRSRIQTIYQPGSFTPLIRVETATGEL
                     AKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGL
                     TVEQMKNQMDPVYTPARKIHLYHCDHRGLPLALISTEGATAWCAEYDEWGNLLSDENP
                     HHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVI
                     NVDPQGLVDINLYPESDLIHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDLAY
                     LIKFDGNYKDGMTVWLFSCNTGKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNGKL
                     KMDTVLTAPTNLNSNKDLMAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          992 a         1194 c         1113 g         1294 t
ORIGIN      
        1 atcaatgatt ttttattctt atcttttatt ttatttactg tttcaatgca atcagcggca
       61 agttggagtg gatttagatt tttcagatat ggtcttatga tctttccctt acttaataaa
      121 tagcatgtat aggattcgga aagtccagca tctattttgt acctcattat attagataag
      181 gatgtgagaa attccttgtt atcattcgca gccatatatg ccagtccgag ttcagcctct
      241 gcggccttac aggaaaaata gcttagtgaa cacgcttttc gtccctcttt agtactggct
      301 gatattagtg cccataatat tgctttgtct gccaaatcta actctctctg aaattgtctt
      361 gcagtaactg cactcgcaga aatagagaaa aaaaatacaa ttgttaataa ccttgccata
      421 ttttttagtc cgtaactgtt aatgtgctat ctggctctga aaatattaac tcatttaatt
      481 tttgcatgtc attagataaa ccagttatac aaccctcgga acagaatcga ggtcctaaag
      541 acggaccaaa agcgtttaaa caactatgta ttgaaaatcc attgcgatta taagttcctg
      601 tattaactga cggtgctaat cgtatgccgt caggttttct attgcttgct cccgggtcat
      661 taacattcca actccatagg ccttgaggta ttggccatct atttataaat gtgtacaacg
      721 gggattattt ttacattgca tattattacc agaagcgact ggaatactaa gcacatcata
      781 aaaattatca tcacatggta tatctgaaac acacgaaagc atacctaaag ttatgctgta
      841 taaacaggtg gctaatccca aggggatcga ttccagaaat cgggttcaac ggatactgat
      901 aaaaattcca cctaccttcc agcccaatcg gagcctgagt gttataccgc ccctgctgcg
      961 gttaatgtta ccgataatag ttatagtaca gccctgattc atcatcatac tactgctgcg
     1021 gcagtctgat gaactattgc ttttacggat tctccttatt cttccttcta tccattcgct
     1081 ttagcttttt gagctcttga agtttgctct ttgcatcaat attaccgtta tttacagcga
     1141 attccatcca ataaatagct ctattcaaat tataatattc tgataatgtt ggatttgtat
     1201 ctgataaaaa gaccccataa ttaaactgcg ctgtaacatt cccctgagat gctgatcttt
     1261 ccaagaatcg caattgttta tcaatattat ttttagtaaa gcagtaatat tgatatagtc
     1321 gaaatgatgc ctctgaatca ccatgttgtg atttatactt cagtttcatc agcttttctt
     1381 cacttaaatc ataggtcatt gctgtagatc ccatcacact ttcgttttct tttattttca
     1441 catcagcacc atgcaattga ccaataagag aaaaataaaa aaatagtaac aagcatgctt
     1501 tcatacatct acaacctatc ttatatcact gggtttttct ggcgtacctt gcatattaga
     1561 aatggggtgc ccagatggcc catatgttat ccaattacca aggtcttttg ttgttatagc
     1621 cattagatcc ttatttgaat taaggttcgt tggtgctgtt agcactgtat ccatttttaa
     1681 cttaccatta gttcctcgcc cccaccacgt ccatagcgtg tcaggtccta ttacatttgt
     1741 atgtaactct ttagctaatt ggctagcaaa tgaattttgt cctttacctg tattacaaga
     1801 aaataaccaa actgtcatcc catctttata attcccatca aatttaatta gatatgctag
     1861 atctttagct gtcatgatac tgcgcgttgc ggattcaata gatgtggggg taccatgccc
     1921 cccgattgtg aaaacgcctg ggatattaat ctcatcagct acagaatgga taagatcact
     1981 ttcggggtat aaatttatat caaccaaacc ttgcggatct acatttatga ccggattcaa
     2041 cggatactga taaaaattcc atcccccctt cagcccaatc ggatcctgag tgatataccg
     2101 cccctgcagc gggtcataat agcggtggcg gttgtaatac aggccggact cctcatcata
     2161 ctgctgaccc ggcaggcgaa tgagctgctg cagatgatgc gggttctcat cactcagcag
     2221 gttgccccat tcatcatatt ctgcgcacca cgctgttgcc ccttccgtgc tgatgagcgc
     2281 cagcggcagg ccgcgatggt cgcagtggta caggtggatt tttcgcgccg gcgtgtacac
     2341 cgggtccatc tggtttttca tctgctccac ggtcaggccg cacgatgcca gccagcggcg
     2401 gctttcctca ctcacccggt cagccaggat ttcactttcc agccggtcga gcatctgcac
     2461 cagcaccggc gggaacacca cactgccacc gtcttcgccg ccggactgct gaagggtatc
     2521 cgccaggctg cggcgctgcg ttttcgccag ctcaccggtg gcggtttcaa ctctgatgag
     2581 tggcgtgaag ctccccggct gataaatcgt ctggatgcgg ctcctgtcgt tctgtattgt
     2641 ggtcagccgg tcgccgtccc agccgtacca ggtcacttcc ggtttccgtg acagcgacat
     2701 ccagcccgtc aggtcacgtt cacgccgcca cacccgtttt gccaccctgc ggcccagcgg
     2761 gtcgtaaaga tagcgacttt cgaccagcgg ctctgcatat tgtgtccgcg tgtagtgcac
     2821 cagccggtgc tgactgtcgt aatggtaccg gtgggtgcgc tcatcatccg tacggataac
     2881 cccttccggg atgaggtcgg ttttctccgt cagcctgccg taacggtcat accggtaaag
     2941 atagtgcgcg tcacgggcga tacggttatc cggccacatg ctgagggtgc tgtccgggtg
     3001 cagctccggg tcgggcaggc ggttacctgc cgggtctgtg gcatacggga tgcggatatc
     3061 aaggttcgct gcgatggtgt gaacgccggt cagcctgccg gtggtgctgt agctgtaact
     3121 ccgggtctgg cgcgggctgc tgatgcggat gagttcgccg ttgtcgttcc aggtgtaatc
     3181 gcggtcatac tgcaggctgt tcaggtgctg gctctgtaac tgcccggcag gggtataagc
     3241 ggtggtgagt tcatagcggc cgaagcggcg cagcgtttcc cggtgcaggc ggtcgcgggt
     3301 gaaatccacc agcggtgtgt cgccgagctt catgcctgcc aggtaaccgc tgccgtaggc
     3361 cagccattcc acggcgggca ggctgtccgg tatacagcgg ttcgccagtc cctgcgcgtt
     3421 gtaagcgtgt ctggtctcat gctgccagag cagtgcttcc ggctgcgggt gatgcaccgt
     3481 ctggcgctca ccggtcagcc ggcctttctc atcatacctg taatgcaccg tcacccggtg
     3541 cccttcgctg agatggctga tgtctgtcag ccagccacgt ttgtcatact gccactgctc
     3601 tgcggtttcg ccattcacgg tgcggtgcgt gaggcggtct gcttcgtcat agtgccagtg
     3661 ggtgaccagc ccctcatcct cgctgcggat aagtttgccg gtcaggtcgt ggtgataacg
     3721 ctgtgtgcgg ccgtcaaagc cggtttcctg tatcagccgg tcgagtacat cgtaacggaa
     3781 ggtggtgtgg ctgccgtttt cactggtcag gcggatgacc cgtccggcag catcgtattc
     3841 catactgcgc gtcagcccgc cctgcgtggt acagatggct tttccccacg catcgtactg
     3901 tgtcccgttt ctgctgccgt ccggggcaat gacggcggtc aggtcaccgg cggcgttgta
     3961 ttcataccgc atttcatggc cctgcgtgtc tttcaccgca attaactgtc cacggctgtc
     4021 gtatgcgcgg tactggctca gcccttcctc gcggtgcacc gccgtcacct gaccaaaacg
     4081 gtcatggtca taacgggtta cataaccgga acagtcagtg acggtcagca actgaccgta
     4141 acggctccat gtcatggttt tccggctgcc ggtggcatct tccgttgcgc agggtaagtc
     4201 actgtgtgga ttatcataac ggtagcgggt gatatcgccg tcaggggcgg tttcctgaat
     4261 cagacggccg gattcatcat atttgcggcg catttccagc ccgtcaggcc cggtggctga
     4321 cgttaactgg ctgtggtggt tatagtaaaa cgccgatgcc ctgccatccg gcgtggtgat
     4381 gcgcgtgatg aggcccgtca ccacatccgg gctgtattct gttgtccggc ctgcggcatc
     4441 cgtctgtgtc ctgagcctgc ccacggcgtc aaactgactc tgcgtgacgc tgccgtccgc
     4501 gtgttccttt ttcaccaccc gcttcagccc ggcttcaccc tgcgtgtgca gcacttcacg
     4561 gcggttcagg ctgtcggtga tggtgatgcg gtc
//
LOCUS       DAZKHC020000202         4522 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZKHC020000202 DAZKHC020000000
VERSION     DAZKHC020000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.203"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            170..1327
                     /gene="acrE"
                     /locus_tag="ACHOSM_RS23635"
                     /old_locus_tag="pgaptmp_004727"
     CDS             170..1327
                     /gene="acrE"
                     /locus_tag="ACHOSM_RS23635"
                     /old_locus_tag="pgaptmp_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HGU4561068.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKVHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1339..4442
                     /gene="acrF"
                     /locus_tag="ACHOSM_RS23640"
                     /old_locus_tag="pgaptmp_004728"
                     /pseudo
     CDS             1339..4442
                     /gene="acrF"
                     /locus_tag="ACHOSM_RS23640"
                     /old_locus_tag="pgaptmp_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
BASE COUNT         1094 a         1097 c         1192 g         1139 t
ORIGIN      
        1 atattaatct ttttttgtgt ttatgtgcct tgagacgcca gtattcataa ctattcctta
       61 catcgacgaa tgataatttg taggatagca aactgtattt ttctttctgc gagttaacgc
      121 gttgcctttc tgggtaaata acgcgctttt ggttttttga ggaatagtaa tgacgaaaca
      181 tgccaggttt ttcctcctgc cctcctttat tctgatctcc gcggctttaa tcgccggttg
      241 taacgataag ggagaagaga aagttcacgt cggtgaaccg caggttaccg ttcatattgt
      301 gaaaacggcc ccgttagaag ttaagactga attaccaggc cgcaccaatg cttaccgtat
      361 agccgaagtt cgcccacagg ttagcgggat cgtactgaat cgtaatttca ctgaaggcag
      421 cgatgtacaa gcaggccagt ccctgtacca gatcgatccc gcgacctatc aggcaaatta
      481 tgacagcgcg aaaggcgaac tggcgaaaag tgaagccgcc gccgccatcg cgcatttgac
      541 ggtaaaacgt tacgttccgc tcgtgggtac gaaatacatc agccagcagg agtacgacca
      601 ggccattgct gatgctcgcc aggccgatgc cgccgtgatt gccgcaaaag ccacagtcga
      661 aagcgctcgc atcaatctcg cttataccaa agtgactgcg ccaattagcg gacgtatcgg
      721 caaatcgacc gtgaccgaag gcgctcttgt cactaatggg caaacgactg aactggcgac
      781 tgtccagcag ctcgatccta tctacgttga tgtgacccaa tccagcaacg attttatgag
      841 gctgaagcaa tccgtagagc aaggaaattt gcataaggaa aacgccacca gcaacgtaga
      901 gttggtcatg gaaaacggtc aaacctatcc cctgaaaggt acgctgcaat tctctgatgt
      961 gaccgttgat gaaagcaccg gctccataac cctacgtgct gtcttcccta acccgcaaca
     1021 tacgcttttg ccgggtatgt ttgtgcgtgc acggattgat gaaggcgtcc aacctgacgc
     1081 cattcttatc ccgcaacaag gcgttagccg cacaccgcgt ggtgatgcaa ccgtgctgat
     1141 tgttaacgat aaaagtcagg tggaagcgcg ccctgtcgtt gccagtcagg cgattggcga
     1201 taaatggttg attagcgaag gtctgaaatc tggcgatcaa gtcattgtca gcggcctgca
     1261 aaaagcgcgt ccgggagagc aggttaaagc cactaccgat acccccgcag atactgcatc
     1321 gaagtaaggt aatctgacat ggcaaacttt tttattcgac gaccgatatt tgcatgggtg
     1381 ctggccatta ttctgatgat ggcgggcgca ctggcgatcc tacaattgcc cgtcgctcag
     1441 tatccaacca ttgcaccgcc tgcggtttct gtttcggcaa actatccagg tgctgatgcg
     1501 cagaccgtgc aggatacggt gacgcaggtt atcgaacaga atatgaatgg tatcgataac
     1561 ctgatgtata tgtcctccac cagcgattcc gccggtagcg tgacaattac ccttaccttc
     1621 cagtccggga ccgatcctga tatcgcgcaa gtgcaggtgc agaacaaact ccagctcgcc
     1681 acgccgttgc tgccgcagga ggttcagcag caggggatca gtgttgaaaa gtccagtagc
     1741 agctatttga tggtggcggg ctttgtctct gataacccag acaccacaca ggacgatatc
     1801 ttggactatg tggcctctaa cgttaaagat acgcttagcc gtctgaatgg cgtcggtgac
     1861 gtacagcttt tcggcgcaca gtatgcgatg cgtatctggc tggacgccga tctgctaaac
     1921 aaatataaac tgacaccggt tgatgtgatt aaccagttga aggtacagaa cgatcagatc
     1981 gctgccggac agttgggcgg acgccagcgt taccagggca acagttgaat gcctcgatta
     2041 ttgctcaaac gcggttaaaa aatccggaag aattcggcaa agtgaccctg cgcgtaaaca
     2101 gtgacggctc ggtggtacgc ctgaaagatg tcgcacgggt tgaacttggc ggtgaaaact
     2161 ataacgttat cgctcgtatc aacggaaaac cggcggcggg cctggggatt aagctggcaa
     2221 ccggcgcgaa tgctctcgat accgcgaaag ccattaaggc aaaactggcg gaattacagc
     2281 cattcttccc gcagggaatg aaggttctct acccttatga caccacgcca ttcgtccagc
     2341 tttctattca cgaagtggta aaaacgctgt tcgaagccat tatgctggtg ttcctggtga
     2401 tgtatctgtt cttgcagaat atgcgagcaa cgctgatccc caccattgcg gtacccgtgg
     2461 tgttgttagg gacgtttgcc atcctcgccg cttttggtta ctccatcaac acactaacga
     2521 tgttcgggat ggtgcttgcc atcgggctgc tcgtcgatga tgcgatagtg gtggtggaga
     2581 acgtcgagcg cgtgatgatg gaggataagc tcccgccaaa agaagcgacg gaaaaatcaa
     2641 tgtcgcaaat ccagggcgca ctggtgggta tcgcgatggt gctgtcagcg gtatttattc
     2701 cgatggcatt cttcggcggt tctactgggg caatttatcg ccagttctct atcaccatcg
     2761 tttcagcaat ggcgctttct gttctggtgg cattgattct tacccctgcg ttatgtgcaa
     2821 cgctgcttaa acccgtctct gctgagcatc acgaaaataa gggcggtttc ttcggctggt
     2881 ttaatactac cttcgatcat agcgttaacc actacaccaa cagcgtcggc aaaatcctcg
     2941 gttccacagg acgatattta ctgatctatg cgctgattgt tgcaggaatg gtggtgttgt
     3001 ttttacgtct tccgtcttcc ttcttacctg aagaggatca gggtgtcttt ctgaccatga
     3061 ttcagttacc cgctggcgcg acgcaagagc ggacgcaaaa agtgttggat caagtgacgg
     3121 attactatct gaagaacgag aaagcgaacg ttgaaagtgt ctttacggtt aacggcttta
     3181 gcttcagcgg ccaggcacaa aacgccggta tggccttcgt cagtctgaaa ccgtgggaag
     3241 agcgtaatgg tgatgaaaac agtgcggaag cggtaatcca tcgtgccaaa atggaattgg
     3301 gcaagatccg cgacggtttt gtcattccat tcaatatgcc agccattgtt gaactgggca
     3361 cggcaacggg tttcgacttt gagttaattg atcaggctgg gctgggtcac gatgccctga
     3421 cccaggcccg taaccagttg cttggcatgg cggcgcaaca tcctgccagc ttagtcagcg
     3481 tgcgtcctaa tggtctggaa gacaccgcgc agtttaaact ggaagttgac caggaaaagg
     3541 cgcaggcatt aggtgtttca ctttctgaca tcaatcagac catttcaacg gcgctgggtg
     3601 ggacttacgt taacgacttc atcgaccgtg gtcgcgtgaa aaaggtgtat gttcaggcgg
     3661 atgccaaatt ccgtatgctg ccagaagatg tcgataaact ttatgtccgc agcgccaacg
     3721 gcgaaatggt gccattctct gcctttacca cttcacattg ggtgtatggc tctccgcgac
     3781 tggaacgcta caacggtctg ccgtcaatgg agattcaggg ggaagccgcg ccaggaacca
     3841 gttccggcga tgccatggcg ttgatggaaa accttgcgtc aaaattacct gcgggcattg
     3901 gttatgactg gacgggtatg tcgtatcagg aacgcttatc gggaaaccag gctcccgctc
     3961 tggtagcaat ttcctttgtg gttgttttcc tgtgccttgc tgcactctat gaaagctggt
     4021 caattcctgt ctcggttatg ttggtcgtgc cgttagggat tgtcggcgtg ctgctggcgg
     4081 cgacactctt taatcaaaaa aatgacgtct actttatggt gggcttgcta acgacaattg
     4141 gcttgtcggc caaaaacgct attttgattg ttgagttcgc taaagatctc atggagaaag
     4201 agggtaaagg tgttgttgaa gcgacactaa tggcagtacg tatgcgtctg cgtcctatcc
     4261 tgatgacctc tctcgccttt attctcggcg tattaccgct agctatcagt aacggtgccg
     4321 gcagtggcgc gcagaacgca gtgggtatcg gggtaatggg cggaatggtc tctgcaacgt
     4381 tgctggcaat cttcttcgta ccggtgttct ttgtggtgat ccgccgttgc tttaaaggat
     4441 aaatcagaaa cataaaggca ctttcgagtg cctttattat tttcagtgaa acccataaaa
     4501 attaataaga tattcttcca ct
//
LOCUS       DAZKHC020000203         4511 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZKHC020000203 DAZKHC020000000
VERSION     DAZKHC020000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.204"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..340)
                     /locus_tag="ACHOSM_RS23645"
                     /old_locus_tag="pgaptmp_004729"
     CDS             complement(<1..340)
                     /locus_tag="ACHOSM_RS23645"
                     /old_locus_tag="pgaptmp_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HGU4561069.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTMIL"
     gene            568..1176
                     /locus_tag="ACHOSM_RS23650"
                     /old_locus_tag="pgaptmp_004730"
     CDS             568..1176
                     /locus_tag="ACHOSM_RS23650"
                     /old_locus_tag="pgaptmp_004730"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HGU4561070.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1274..2665
                     /gene="selA"
                     /locus_tag="ACHOSM_RS23655"
                     /old_locus_tag="pgaptmp_004731"
     CDS             1274..2665
                     /gene="selA"
                     /locus_tag="ACHOSM_RS23655"
                     /old_locus_tag="pgaptmp_004731"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HGU4561071.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2662..4506
                     /gene="selB"
                     /locus_tag="ACHOSM_RS23660"
                     /old_locus_tag="pgaptmp_004732"
     CDS             2662..4506
                     /gene="selB"
                     /locus_tag="ACHOSM_RS23660"
                     /old_locus_tag="pgaptmp_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HGU4561072.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          998 a         1157 c         1343 g         1013 t
ORIGIN      
        1 tgagtatcat tgtgttatcg cgcagctgta agcggatatc cgcaggcatt ttccagccgg
       61 ggccgaggct ccccaccggc gcgggcgttt ttgtccggta actgctgtag gtgcgggaga
      121 ggatgaacgg cagcgggccg ggcagggcga tgtcggtttc accgggaagg acctttgcac
      181 cgagcagcgg attgaccgga tggccggacg tcaccccgcc ggggcacacc gaacaggcca
      241 cgccggtggg ggcaccaatg cgcaccccgg ctgaaccctg aacaatgcta ccgccatact
      301 gcgtcatgtc gccctgacgc gccgccggtt ttccgctcat ttctcatcct tgttttatcg
      361 ggttatttgt ctgctgacag gcaaaaccct acagatttat aagaaagcct taagtgagat
      421 atccatcaga tagaaacaat taggaaaata tttatatcac catgataaat atgtgattta
      481 tttggaagta acaaaccatc aacattttct gccctttttc ccatcaccag gccatactga
      541 tatttttcct cgcacaaagg actcggcatg aaactcgtcg gtagctacac cagcccgttt
      601 gtacgcaaac tttctattct gttgttagaa aagggcataa ctttcgaatt tattaatgaa
      661 ctgccctata acgcggacaa cggcgtggcg caatttaacc cgttaggaaa agtgccggtg
      721 ctggtgaccg aagagggcga atgttggttt gattcgccga tcatcgctga atatattgaa
      781 ttaatgaatg tcgctccggc gatgttgccg cgcgatccgc tggagtcgtt gcgggtgcgc
      841 aaaattgagg cgctggcgga tggcattatg gatgccgggc tggtatcggt gcgtgaacag
      901 gcgcgtccgg cggcgcagca gtctgaagat gaattgttac gccagcggga gaaaatcaac
      961 cgcagtctgg atgtgctgga aggatatctg gtcgatggca cactcaaaac cgatacggtc
     1021 aatctggcga ctatcgccat tgcctgtgct gtcggatatc tcaatttccg ccgcgttgcg
     1081 ccaggttggt gtgttgaccg cccacactta gtcaaactgg tcgaaaacct gtttagccgc
     1141 gaaagttttg ctcgcaccga accgccaaag gcttgatgcg cgatatgtcc tcctgaccca
     1201 tctcacgtta caatccgtgg ttatgttaaa cgcccttctc cgtgtgagag ggccttgatc
     1261 agccaggttt cctatgacaa ccgaaacgcg ttccctctat agtcaacttc cggctattga
     1321 tcgcttattg cgcgatagct ccttcctttc tttgcgtgat acttatggtc acacccgcgt
     1381 ggtggaattg ttgcgtcaga tgctcgacga agcgcgagaa gtgattcgtg acagccagac
     1441 gcttcctgcg tggtgtgaaa actgggcgca agaagtcgat gcccggttga cgaaagaggc
     1501 gcagagcgcg ctgcgtccgg tgatcaacct gacgggaacc gtgctgcata ccaaccttgg
     1561 acgagcttta caggcggaag ccgcggtgga agccgttgcg caggctatgc gttcgccagt
     1621 gaccctcgag tacgatctgg acgacgccgg acgcggacat cgcgatcggg cgctggcgca
     1681 gctgctgtgc cgtattacgg gggcggaaga tgcctgtatc gtcaataaca atgcggcggc
     1741 ggtgttattg atgttggcgg ccactgccag cgggaaagag gtggtggtat ctcgtggcga
     1801 actggtggag attggcggcg cgtttcgtat tcccgatgtt atgcgtcagg caggctgcac
     1861 cctacacgaa gtagggacca ccaaccgcac gcacgcgaat gattatcgtc aggcggtgaa
     1921 tgaaaatacc gcactgttga tgaaagtaca taccagtaac tacagcattc aggggttcac
     1981 caaagcgata gacgaagctg aactggtggc gctcggcaaa gagctggatg ttccagtggt
     2041 gactgattta ggcagtggct cgctggtcga tcttagccag tacggtttgc cgaaagagcc
     2101 aatgccgcag gagttgattg cggcgggcgt cagtctggtg agcttctccg gcgacaagtt
     2161 gttaggcggg ccgcaggcag ggattatcgt tggtaaaaaa gagatgatcg cccgcctgca
     2221 aagccacccg ctgaagcgtg cattacgcgc ggataaaatg accctcgcgg cgctggaagc
     2281 cacgttgcgt ctttatttac accctgaagc tctgagtgaa aaattaccga ccctgcgcct
     2341 gcttacccgc agcgcagagg tcattcaaat ccaggcacaa cgtttacagg ctccccttgc
     2401 cgcacattac ggcgcggagt ttgcggtaca ggttatgcca tgtctttcgc agattggcag
     2461 tggttcgctg ccggttgatc gcctgccgag cgcggcatta acgtttacac cccatgatgg
     2521 acgcggtagc caccttgagt cattagccgc ccgctggcgt gaattgccag tgccggtgat
     2581 tggtcgtatt tatgacggac gattgtggct ggatttacgc tgccttgaag atgagcaacg
     2641 gtttttggag atgttgttga aatgattatt gcgactgccg gacacgttga ccacggcaaa
     2701 acaaccttat tgcaggcgat tactggcgta aatgctgacc gtctgccgga agaaaaaaag
     2761 cgcggcatga ccatcgatct cggctatgcc tactggccgc agccggatgg tcgcgtgcct
     2821 ggttttatcg acgtacccgg tcatgaaaag tttctttcca acatgctggc gggcgttggt
     2881 ggtatcgatc acgcgctgtt ggtggtggcg tgcgatgacg gcgtgatggc acagacccgt
     2941 gagcatctgg cgattttgca gctgaccggt aacccgatgc tgacagtggc gctgaccaaa
     3001 gctgatcgcg tggacgaagc gcgtgttgat gaggttgaac gccaggtaaa ggaggttctg
     3061 cgggaatacg gttttgctga ggcaaaactg tttatcaccg cagcaaccga aggtcgggga
     3121 attgatgccc tgcgcgagca tctgcttcag ttgccggaac gcgagcacgc cagccaacat
     3181 agtttccgcc tcgcgattga ccgcgcattt accgtaaaag gtgccgggct ggtcgtcacc
     3241 ggtacggcgt taagcgggga agtgaaggta ggcgattcac tctggctgac tggtgtaaat
     3301 aaaccgatgc gtgtacgtgc gctgcatgca caaaaccagc caacagaaac cgcccatgcc
     3361 gggcagcgta tcgcgcttaa catcgcgggt gatgcggaaa aagagcagat taaccgtggc
     3421 gactggctgc ttgccgatgc gccgccagag ccgttcacac gggtgattgt cgagcttcaa
     3481 acccatacgc cgctgaccca gtggcagccg ctgcatattc accacgccgc cagccacgtt
     3541 acgggacgcg tttcgctgct ggaagataac cttgccgagc tggtgttcga cacgccgctg
     3601 tggctggcgg ataacgatcg cttagtgctg cgcgatatct cggcccgcaa cacgctggcg
     3661 ggggcgcgtg tggtgatgct taacccgccg cgtcgcggta aacgtaagcc ggaatatctg
     3721 caatggctgg cgtctcttgc acgggcgcag agcgatgccg atgcgttatc tgttcatctg
     3781 gaacgcggcg cggttaacct tgcggatttc gcctgggcgc gccagctcaa cggcgaaggg
     3841 atgcgcgaat tgctgcaaca gcctggctat attcaggctg gttatagctt gttgaatgcg
     3901 ccggttgccg ctcgctggca acggaaaatt ctcgacacat tagcgactta tcatgagcaa
     3961 catcgcgatg aacctgggcc tgggcgcgaa cgtctgcgac gtatggcgtt gccaatggaa
     4021 gatgaagcgc tagtactgtt gctgattgaa aagatgcgcg aaagcggcga catcctcagt
     4081 catcacggtt ggctgcatct gccggatcac aaagcgggct tcagcgaaga gcagcaggcc
     4141 atctggcaaa aagcagagcc gctgtttggt gacgagccgt ggtgggtgcg tgatctggca
     4201 aaagagacgg gtaccgacga gcaggcaatg cgcctgacgc tacgccaggc ggcgcagcaa
     4261 ggaataatta ccgcgatcgt caaagatcgt tattaccgta acgatcggat tgtcgaattt
     4321 gccaatatga tccgcgatct cgatcaggag tgtggttcaa cctgcgcggc ggatttccgc
     4381 gatcgcttag gcgtaggccg aaagctggca attcagattc tggaatattt tgaccgcatt
     4441 ggctttacgc gtcgtcgtgg aaatgatcat ttattgcgcg acgcattatt atttccggaa
     4501 aaataaggaa a
//
LOCUS       DAZKHC020000204         4500 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZKHC020000204 DAZKHC020000000
VERSION     DAZKHC020000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.205"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..353)
                     /locus_tag="ACHOSM_RS23665"
                     /old_locus_tag="pgaptmp_004733"
     CDS             complement(<1..353)
                     /locus_tag="ACHOSM_RS23665"
                     /old_locus_tag="pgaptmp_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU4561073.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTR"
     gene            complement(468..543)
                     /locus_tag="ACHOSM_RS23670"
                     /old_locus_tag="pgaptmp_004734"
     tRNA            complement(468..543)
                     /locus_tag="ACHOSM_RS23670"
                     /old_locus_tag="pgaptmp_004734"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(508..510),aa:Thr,seq:ggt)
     gene            complement(550..624)
                     /locus_tag="ACHOSM_RS23675"
                     /old_locus_tag="pgaptmp_004735"
     tRNA            complement(550..624)
                     /locus_tag="ACHOSM_RS23675"
                     /old_locus_tag="pgaptmp_004735"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(589..591),aa:Gly,seq:tcc)
     gene            complement(741..825)
                     /locus_tag="ACHOSM_RS23680"
                     /old_locus_tag="pgaptmp_004736"
     tRNA            complement(741..825)
                     /locus_tag="ACHOSM_RS23680"
                     /old_locus_tag="pgaptmp_004736"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(789..791),aa:Tyr,seq:gta)
     gene            complement(834..909)
                     /locus_tag="ACHOSM_RS23685"
                     /old_locus_tag="pgaptmp_004737"
     tRNA            complement(834..909)
                     /locus_tag="ACHOSM_RS23685"
                     /old_locus_tag="pgaptmp_004737"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(874..876),aa:Thr,seq:tgt)
     gene            1271..2221
                     /gene="coaA"
                     /locus_tag="ACHOSM_RS23690"
                     /old_locus_tag="pgaptmp_004738"
     CDS             1271..2221
                     /gene="coaA"
                     /locus_tag="ACHOSM_RS23690"
                     /old_locus_tag="pgaptmp_004738"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGU4561074.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2250..3215)
                     /gene="birA"
                     /locus_tag="ACHOSM_RS23695"
                     /old_locus_tag="pgaptmp_004739"
     CDS             complement(2250..3215)
                     /gene="birA"
                     /locus_tag="ACHOSM_RS23695"
                     /old_locus_tag="pgaptmp_004739"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGU4561075.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3212..4240)
                     /gene="murB"
                     /locus_tag="ACHOSM_RS23700"
                     /old_locus_tag="pgaptmp_004740"
     CDS             complement(3212..4240)
                     /gene="murB"
                     /locus_tag="ACHOSM_RS23700"
                     /old_locus_tag="pgaptmp_004740"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGU4561076.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1143 a         1135 c         1022 g         1200 t
ORIGIN      
        1 tcacgagtct gcggcatcgg gccgtcagtc gcagcaacta ccaggatcgc gccgtccatc
       61 tgagcagcac cggtgatcat gtttttaaca tagtcggcgt gccccgggca gtctacgtgt
      121 gcgtagtgac gggtcggggt gtcgtattca acgtgagaag tgttgatggt gataccacga
      181 gctttttctt ccggcgcgtt atcgatctgg tcgaatgcgc gagcagcacc gccgtaggtt
      241 ttagccagta cggtagtgat tgcagcggtc agcgttgttt taccatggtc aacgtggccg
      301 atagtaccga cgttaacgtg cggttttgta cgttcaaact tttctttaga catcgattgt
      361 ccctctaaga cacggataaa tcggtgatat caccacatca accaggcaac atgcccgact
      421 tgttgaatgc aataaacaga aggaaaaaac agggaggaga aaagaagtgg tgctgatagg
      481 cagattcgaa ctgccgacct cacccttacc aagggtgcgc tctaccaact gagctatatc
      541 agcacatctt ggagcgggca gcgggaatcg aacccgcatc atcagcttgg aaggctgagg
      601 taatagccat tatacgatgc ccgcatcctg gaactcggct acctgatttt cattctgcac
      661 tgaatatcga gagaagctct ctttattcga gccggtaagc gaacttatcg tctcgggcta
      721 cgccatcgcg tggccgaaat tggtggtggg ggaaggattc gaaccttcga agtctgtgac
      781 ggcagattta cagtctgctc cctttggccg ctcgggaacc ccaccggact tgatggtgcc
      841 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta cctactgagc
      901 taagtcggca tcaagtagcg cgcattctat ggagacatgc gagttcatgc aactaaaaaa
      961 ttgcataatt tgttttattg atcacatttt atgcgacacg atgaagaaac agccgaacgg
     1021 agggcagatt agcacacttt ttcaacatca ttgtgctcaa caatgcgctc ctgctaaacc
     1081 ataattcttt ttatcagatg gaatatctgt cacattgctt ttcaacgata gcttcctggc
     1141 agagattttt tcttattatt cctccccatc tggtgttacc ctcctgccca ttaacccatt
     1201 caacagaact gtgacgcgcc atggcaaata tcgctttgcc gatagagcta tgaccgccag
     1261 aaacatgctt atgagtataa aagagcaaac gttaatgacg ccttacctac agtttgaccg
     1321 caaccagtgg gcagctctgc gtgattccgt acctatgacg ttatcggaag atgagatcgc
     1381 ccgtctcaaa ggtattaatg aagatctctc gttagaagaa gttgccgaga tctatttacc
     1441 tttgtcacgt ttgctgaact tctatataag ctcgaatctg cgccgtcagg cagttctgga
     1501 acagtttctt ggtaccaacg ggcaacgcat tccttacatt atcagtattg ctggcagtgt
     1561 cgcggtgggg aaaagtacaa ccgcccgtgt attgcaggcg ctattaagcc gttggccgga
     1621 acatcgtcgt gttgaactga tcactacaga tggcttcctt caccctaatc aggttctgaa
     1681 agaacgtggt ctgatgaaga agaaaggctt cccggaatcg tatgatatgc atcgcctggt
     1741 gaagtttgtt tccgatctca aatccggcgt gccaaacgtt acagcccctg tttactcgca
     1801 tcttatttat gatgtgatcc cggatggaga taaaacggtt gttcagcctg atattttaat
     1861 tcttgaaggg ttaaatgtct tacagagcgg gatggattat ccacacgatc cacatcatgt
     1921 atttgtttct gattttgtcg atttttcgat atatgttgat gcaccggaag acttacttca
     1981 gacgtggtat atcaaccgtt ttctgaaatt ccgcgaaggg gcttttaccg acccggattc
     2041 ctattttcat aactacgcga aattaactaa agaagaagcg attaagactg ccatgacatt
     2101 gtggaaagag atcaactggc tgaacttaaa gcaaaatatt ctacctactc gtgagcgcgc
     2161 cagtttaatc ctgacgaaaa gtgctaatca tgcggtagaa gaggtcagac tacgcaaata
     2221 atttgcaggg gagtgaatac tcccctttct tatttttctg cactacgcag ggatatttca
     2281 ccgcccatcc agggttttat tattccatcc tgctcaagta ataaagcccc ctgtttgtct
     2341 attccgcgtg aaatgccaaa tatttcttta tcaccaatga taagtttcac tgggcgatta
     2401 ataaaattat ccagcttttc ccagcgcgac agataaggtg ccaatccttc ttgttcgaag
     2461 agttccaacg cagcacgtaa ttcacgtatt agcatggccg ccaacgtatt acgatcgaga
     2521 ttgatccccg cttcctgcag cgtgatccac ccctgattaa cgacactctc ttcaacacgg
     2581 cgcattgcca tgttgatccc ggctccaatg actatttgcg ccgcatcgcc agttttgcca
     2641 gtcagctcca caagaatgcc tgccagcttg cgatcctgca gatagaggtc attaggccat
     2701 ttgacacgaa ctttatctgc tcccagcttg cgtaatactt ccgccatcac gataccgata
     2761 accagactta aaccaatcgc cgccgccggg ccttgttcca gacgccagaa catcgacaaa
     2821 tataagtttg cgccaaaagg cgaaaaccat ttccgccccc ggcgaccacg gccagcctgc
     2881 tggtattctg caacacaggc atcgcccgat ttaagctctc cgatacgatc aagaaggtac
     2941 tgattcgtgg agtcaataac tggcagcacg gctacactac cgccatccag ctgacccaat
     3001 atctgttcag cattaagtaa ctggatgggc tcaggcaggc tgtatccttt acccggaacg
     3061 gtaaagacat caacgcccca gtcacgcagt gtctgaatgt gtttattaat agccgcccgg
     3121 ctcattccca gcgtttcacc caactgctcg ccagagtgaa attcaccgtt cgctaacagg
     3181 gcaatcaatt tcagtggcac ggtgttatcc ttcatgaaat tgtctccact gcgctcactt
     3241 cacctgatgc accaataaag cggacttcag gctcaagcca gacattaaat ttttcaccca
     3301 ctttttgtct tacatggtgt gccagttgca ccacatcttc gctttttgca ttgtcttcat
     3361 taatgagaac taacgcctgt tgacggtgca ccgcagcccc acccatttgc atccctttta
     3421 gctggcactg atcaataagc caacctgctg ccagttttac tgaaccaccc gcctgggggt
     3481 aatttggtgc tgttggaaat tgcgccagta atgctttagc cgtttcggca gatacaacag
     3541 ggtttttgaa gaaactaccg gcattgccat tcacttttgg atcagggagt ttggtggtgc
     3601 gcatatgaca caccgcatta aatacttgct gtggcgttac tgttgtagga tccagacgag
     3661 ttaagtcacc atacgttagt acaggttgcc actcttttgg cagacgcaga cctacggcta
     3721 caatggcgaa gcgatcctgg tattcatgtt taaaaatact gtcgcgatag ccaaaacggc
     3781 actctttggc agttaagcgc acttgcttgc ctgtcgccag ttcaacacaa tcaacataag
     3841 cgcaaactcg ctgtaattct acgccataag caccaatatt ctggataggt gatgagccga
     3901 cacaaccagg aattaatgcc agattttcca gaccaggcat accttcctgc aaagtgtatt
     3961 ttaccaggcg atgccagttt tctccggctc ctacatgtaa ataccacgca tcaggttcat
     4021 catgaatttc gatacctttg atccggttga tgatcaccgt gccacgatag tcttccagaa
     4081 aaagtacatt acttccttca cccagaataa gaacgggttg tccttctgcg gttgcatgct
     4141 gccaggcatt gagtaattgt tgttcgtctt cggcacatac aatgtgctga gcattatgat
     4201 caatgccaaa tgtgttccag ggttttaagg agtggttcat agctgctttc ctgatgcaaa
     4261 aacaaggcta gtttaccgta tctgtggggg gatggcttgt agatatgacg acaggaagag
     4321 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg
     4381 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcgca acgttcaaat
     4441 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
//
LOCUS       DAZKHC020000205         4496 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZKHC020000205 DAZKHC020000000
VERSION     DAZKHC020000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.206"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            9..3128
                     /gene="agn43"
                     /locus_tag="ACHOSM_RS23705"
                     /old_locus_tag="pgaptmp_004741"
                     /gene_synonym="flu"
     CDS             9..3128
                     /gene="agn43"
                     /locus_tag="ACHOSM_RS23705"
                     /old_locus_tag="pgaptmp_004741"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HGU4561077.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGTANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHE
                     GAIATGTVINDKGWQVVKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINK
                     NGRQIVRTEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRADDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAG
                     TTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLT
                     QKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNA
                     DRLVIDGGRATGKTILNLVNAGNSASGLAASGKGIQVVEAINGATTEEGAFVQGNKLQ
                     AGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSGE
                     NNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASSD
                     NNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGHGS
                     AQHVRAGFRLGSHNDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRGD
                     MSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYVHSVSGSSAEGYN
                     GQATLNVTF"
     gene            3249..>4496
                     /locus_tag="ACHOSM_RS23710"
                     /old_locus_tag="pgaptmp_004742"
     CDS             3249..>4496
                     /locus_tag="ACHOSM_RS23710"
                     /old_locus_tag="pgaptmp_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YeeR"
                     /protein_id="HGU4561078.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAER"
BASE COUNT         1067 a         1196 c         1315 g          918 t
ORIGIN      
        1 aaaagctgat gaaacgacat ctgaacacca gctacaggct ggtatggaat cacattacgg
       61 gcaccctggt ggtggcctcc gaactggccc gctcacgggg aaaacgcgcc ggtgtggcgg
      121 ttgcgctgtc tcttgctgct gtcacatcag tcccggcact ggctgctgac acggttgtac
      181 aggcgggaga aaccgtgaac gacggaacac tgacaaatca tgacaaccag attgtcttcg
      241 gtacggccaa cggaatgacc atcagtaccg ggctggagta tgggccggat aacgaggcca
      301 ataccggcgg ccaatggata caaaatggcg gtaccgccaa caacactact gtcaccggtg
      361 gtggtcttca gagagtgaat gccggaggaa gcgtttcaga cacggttatc agtgccggag
      421 gcggacagag ccttcaggga cgggcagtga acaccacgct gaatggtggc gaacagtgga
      481 tgcatgaggg ggcgatagcc acaggaaccg tcattaatga taagggctgg caggtcgtca
      541 agcccggtac agtggcaacg gataccgttg ttaataccgg ggcggaaggg ggaccggatg
      601 cagaaaacgg tgataccggg cagtttgttc gcggggatgc cgtacgcaca accatcaata
      661 aaaacggtcg ccagattgtg agaactgaag gaacggcaaa taccactgtg gtttatgccg
      721 gcggcgacca gactgtacat ggtcacgcac tggataccac gctgaatggg ggataccagt
      781 atgtgcacaa cggcggtaca gcgtctgaca ctgttgtgaa cagtgacggc tggcagattg
      841 tcaaaaacgg gggtgtggcc gggaatacca ccgttaatca gaagggcaga ctgcaggtgg
      901 acgccggtgg tacagccacg aatgtcaccc tgaagcaggg cggcgcactg gttaccagta
      961 cggctgcaac cgttaccggc ataaaccgcc tgggagcatt ctctgttgtg gagggtaaag
     1021 ctgataatgt cgtactggaa aatggcggac gcctggatgt gctgaccgga cacacagcca
     1081 ctaatacccg cgcggatgat ggcggaacgc tggatgtccg caacggtggc accgccacca
     1141 ccgtatccat gggaaatggc ggtgtactgc tggccgattc cggtgccgct gtcagtggta
     1201 cccggagcga cggaaaggca ttcagtatcg gaggcggtca ggcggatgcc ctgatgctgg
     1261 aaaaaggcag ttcattcacg ctgaacgccg gtgatacggc cacggatacc acggtaaatg
     1321 gcggactgtt caccgccagg ggcggcacac tggcgggcac caccacgctg aataacggcg
     1381 ccatacttac cctttccggg aagacggtga acaacgatac cctgaccatc cgtgaaggcg
     1441 atgcactcct gcagggaggc tctctcaccg gtaacggcag cgtggaaaaa tcaggaagtg
     1501 gcacactcac tgtcagcaac accacactca cccagaaagc cgtcaacctg aatgaaggca
     1561 cgctgacgct gaacgacagt accgtcacca cggatgtcat tgctcagcgc ggtacagccc
     1621 tgaagctgac cggcagcact gtgctgaacg gtgccattga ccccacgaat gtcactctcg
     1681 cctctggtgc cacctggaat attcccgata acgccacggt gcagtcggtg gtggatgacc
     1741 tcagccatgc cggacagatt catttcacct ccacccgcac agggaagttc gtaccggcaa
     1801 ccctgaaagt gaaaaacctg aacggacaga atggcaccat cagcctgcgt gtacgcccgg
     1861 atatggcaca gaacaatgct gacagactgg tcattgacgg tggcagggca accggaaaaa
     1921 ccatcctgaa cctggtgaac gccggcaaca gtgcgtcggg gctggcggcc agcggtaagg
     1981 gtattcaggt ggtggaagcc attaacggtg ccaccacgga ggaaggggcc tttgtccagg
     2041 ggaataagct gcaggccggt gcctttaact actccctcaa ccgggacagt gatgagagct
     2101 ggtatctgcg cagtgaaaat gcttatcgtg cagaagtccc cctgtatgcc tccatgctga
     2161 cacaggcaat ggactatgac cggattctgg caggctcccg cagccatcag accggtgtaa
     2221 gcggtgaaaa taacagcgtc cgtctcagca ttcagggcgg tcatctcggt cacgataaca
     2281 acggcggtat tgcccgtgga gccacgccgg aaagcagcgg cagctatggc ttcgtccgtc
     2341 tggagggtga cctgctcaga acagaggttg ccggtatgtc tgtgaccgcg ggggtatatg
     2401 gtgctgctgg ccattcttcc gttgatgtta aggatgatga cggctcccgt gccggcacgg
     2461 tccgggatga tgccggcagc ctgggcggat acctgaatct gatacacaac gcctccggcc
     2521 tgtgggctga cattgtggcc cagggaaccc gccacagcat gaaagcctca tcggacaata
     2581 acgacttccg cgtccggggc tggggctggc tgggttcgct ggaaaccggt ctgcccttca
     2641 gtatcactga caatctgatg ctggagccgc aactgcagta cacctggcag ggactctccc
     2701 tggatgacgg ccaggataac gccggttatg tgaagttcgg gcatggcagt gcacaacatg
     2761 tgcgtgccgg cttccgtctg ggcagccaca acgatatgaa ctttggtaaa ggcacctcat
     2821 cccgtgacac cctgcgcggc agtgccaaac acagtgtgcg tgaactgccg gtgaactggt
     2881 gggtacagcc ttctgttatc cgcaccttca gctcccgggg ggacatgagc atgggtacag
     2941 ccgcagccgg cagtaacatg acgttctcac cgtcacagaa tggtacgtca ctggacctgc
     3001 aggccggact ggaagcccgt gtccgggaaa atatcaccct gggcgttcag gcaggttatg
     3061 tccacagcgt cagcggcagc agcgctgaag gctataacgg tcaggccacg ctgaatgtga
     3121 ccttctgaca gaaccatcgc ctctctgtgg tcccggtcat catgatcggg acccggaccg
     3181 gcgcaacgga tattcaacgc cacattcgct ggcattaaca ataacatgat attcatcacg
     3241 gagtgactat gttacagata gtcggcgcgc tgatcctgct gatcgcagga tttgccattc
     3301 ttcgcctttt gttcagagca ttaatcagca cggcttctgc gctggcaggg ctcatattgc
     3361 tgtgtctgtt cggcccggcc ttactggctg gctatatcac cgaacgcata acccggttgt
     3421 tccatattcg ctggctggca ggcgtatttc tgacgattgc cggaatgatc atcagcttca
     3481 tgtggggact tgatggtaaa catatcgcgc tggaggctca cacctttgac tctgtgaaat
     3541 ttattctgac caccgctctc gccggtggtc tgctggctgt tcccctgcag atcaaaaaca
     3601 ttcagcagaa cgggatcaca ccagaagata tcagcaagga aattaacggg tattactgct
     3661 gtttttatac tgcctttttc cttatggcgt gttctgcatg cgcaccattg atcgcgttac
     3721 agtacgatat ttcaccgtca ctgatgtggt ggggcgggtt gttgtactgg ctggctgcat
     3781 tagtgacgct gctatgggcg gccagccaaa tccaggcgct gaaaaaactg acctgtgcca
     3841 tcagccagac actggaagaa caaccggtgc tcaacagtaa atcgtggctg accagtttgc
     3901 aaaacgatta cagccttcct gactcactga cggagcgcat ctggctgacg ctcatttctc
     3961 aacggatttc ccggggagag ctgagggaat ttgaactggc agacggaaac tggttactga
     4021 acaatgcctg gtatgaaaga aacatggcag ggtttaacga acagttgaaa gagaacctgt
     4081 cattcacacc tgatgaactg aaaacgctct tccggaaccg cctgaattta tcaccggaag
     4141 cgaatgacga ttttctcgat cgttgcctgg acggcggtga ctggtatccc ttttcagaag
     4201 gtcgccgttt tgtatcattc catcacgtgg atgagcttcg tatctgtgcc tcctgcgggc
     4261 tgacagaagt acatcatgcc ccggaaaatc ataagccgga tccggaatgg tactgctcct
     4321 ctctttgtcg cgaaacagaa acactgtgtc aggaaattta tgaacgccct tacaacagct
     4381 ttatttccga tgcaacggcg aatggtctga ttctcatgaa actgccggaa acctggagta
     4441 caaatgagaa aatgtttgct tccggagggc agggacatgg gtttgccgct gaacgg
//
LOCUS       DAZKHC020000206         4471 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZKHC020000206 DAZKHC020000000
VERSION     DAZKHC020000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.207"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            155..1129
                     /gene="hemB"
                     /locus_tag="ACHOSM_RS23715"
                     /old_locus_tag="pgaptmp_004743"
     CDS             155..1129
                     /gene="hemB"
                     /locus_tag="ACHOSM_RS23715"
                     /old_locus_tag="pgaptmp_004743"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HGU4561079.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1235..2086)
                     /gene="tauD"
                     /locus_tag="ACHOSM_RS23720"
                     /old_locus_tag="pgaptmp_004744"
     CDS             complement(1235..2086)
                     /gene="tauD"
                     /locus_tag="ACHOSM_RS23720"
                     /old_locus_tag="pgaptmp_004744"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HGU4561080.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2083..2556)
                     /locus_tag="ACHOSM_RS23725"
                     /old_locus_tag="pgaptmp_004745"
     CDS             complement(2083..2556)
                     /locus_tag="ACHOSM_RS23725"
                     /old_locus_tag="pgaptmp_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGU4561081.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     AGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAELI
                     AATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2553..3320)
                     /gene="tauB"
                     /locus_tag="ACHOSM_RS23730"
                     /old_locus_tag="pgaptmp_004746"
     CDS             complement(2553..3320)
                     /gene="tauB"
                     /locus_tag="ACHOSM_RS23730"
                     /old_locus_tag="pgaptmp_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HGU4561082.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3333..4295)
                     /gene="tauA"
                     /locus_tag="ACHOSM_RS23735"
                     /old_locus_tag="pgaptmp_004747"
     CDS             complement(3333..4295)
                     /gene="tauA"
                     /locus_tag="ACHOSM_RS23735"
                     /old_locus_tag="pgaptmp_004747"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4561083.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            4273..>4471
                     /locus_tag="ACHOSM_RS23740"
                     /old_locus_tag="pgaptmp_004748"
     CDS             4273..>4471
                     /locus_tag="ACHOSM_RS23740"
                     /old_locus_tag="pgaptmp_004748"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561084.1"
                     /translation="MLRDEIAMIVCSPVLLWEQYSGIKTFIKRISRYRTDDFILSKKT
                     AKERLNMQLLRSNNWENYYTSF"
BASE COUNT         1013 a         1289 c         1143 g         1026 t
ORIGIN      
        1 tcagtgaata tgaaattatt gctatttcta ttacaaagat gtcctggtct ctctttcatt
       61 ctcttcttgt caacatcgcg acaactttcg taaaacactc ctgccccgct tcaggtatac
      121 tgtgcacctt aattccacaa acatcaggca gaccatgaca gacttaatcc aacgccctcg
      181 tcgcctgcgc aaatctcctg cgctgcgcgc tatgtttgaa gagacaacac ttagccttaa
      241 cgacctagtg ttgccgatct ttgttgaaga agaaattgac gactacaaag ccgttgaagc
      301 catgccaggc gtgatgcgca ttcctgagaa acatctggca cgcgaaattg aacgcatcgc
      361 caacgccggt attcgttccg tgatgacttt cggcatctct caccataccg atgaaaccgg
      421 cagcgacgcc tggcgggaag atggactggt ggcgcgtatg tcgcgcatct gcaagcagac
      481 tgtgccagaa atgatcgtca tgtcagacac ttgtttctgt gaatacactt ctcacggtca
      541 ctgcggtgtg ctgtgcgagc atggcgtcga caacgacgcg actctggaaa atttaggcaa
      601 gcaagccgtg gttgcagctg ctgcaggcgc agacttcatc gccccttccg ccgcgatgga
      661 cggccaggta caggcgattc gtcaggcgct ggacgctgcg ggctttaaag atacggcgat
      721 tatgtcgtat tcgaccaagt tcgcctcttc cttttatggt ccgttccgtg aagctgccgg
      781 aagcgcatta aaaggcgacc gcaaaagcta tcagatgaac ccaatgaacc gtcgcgaggc
      841 gattcgtgaa tcactgctgg atgaagccca gggcgcagac tgcctgatgg ttaaacctgc
      901 tggagcatac ctcgacatcg tgcgtgagct gcgtgaacgt actgaattgc cgattggcgc
      961 gtatcaggtg agcggtgagt acgcgatgat taagttcgcc gcgctggcgg gtgctataga
     1021 tgaagagaaa gtcgtgctcg aaagcttagg ttcgattaag cgtgcgggtg cggatctgat
     1081 tttcagctac tttgcgctgg atttggctga gaagaagatt ctgcgttaat ttttttcctc
     1141 tcagaaaggg ggagttaacc tgttgaaagt acggtgaacg ccccctcacc ctaaccctct
     1201 ccccaagggg gcgagggaac cgtccgcact catatcaccc cgcccgataa aacggtttat
     1261 ccccaaggat cgtcgcccga tgcataatcc gccgctgtgg caggtaatcg gcattggcat
     1321 agtgctgagt cacgcggtta tcccaaatcg caatatcatt cggttgccag cgccaacgca
     1381 cctgaaactc cggtttagtg atatgggcga ataaaaagcc taacaaggct tcgctctctt
     1441 tctcgctgac atcgacaatt cgcgtagtaa agccttcatt cacaaacagc gcctgtttac
     1501 cgctcaccgg atgcgttcgc accaccggat gtagcaacgg aggatttttc gcgaccgcct
     1561 cgcgccagcg ttgatgttcc tcctcggttt tgcggtattt gtattccggg aacgatttac
     1621 ggaaatcatg ctccgcacgc agcccgctca gcagctggcg gaagggaaca gagagcgcct
     1681 cataggccgc aataccgctg gtccagagcg tatcaccgcc ggtcgaaggt aactctttag
     1741 ctgccagaat cgcccctgcg ggtggcgttt caataaatgt cacatcggta tgccagttgt
     1801 cgttatccgg cggattatcg ttatgggtat ccagcacgat gatctcgtca accccttcgg
     1861 catgagggta aacagggtga atatgcagtt cgccaaaacg ctgggccagc gcgcgttgct
     1921 gctgcggcgt aatagtttga tcgcgcagaa acaccacctg atggcgcagc accgcatggt
     1981 aaagctgttc aaactgatta tcgcttaacg ggcgcgtcag gtcggcaccc gaaatttgtg
     2041 cgccgatata cggccccagc ggggtaatgc tcagacgttc actcattgta cttctccatg
     2101 ccagggcgtc agacggcgct gtaacgcgcg caaacccagt tctaaaagaa aggcgataat
     2161 cgcaatcacc gcgatccccg ccagcaccac gtcagtcgcg agaaattcac ccgctgactg
     2221 aaccataaat cctaaaccgc gcgtcgcggc aatcagctcc gccgctacca gcgtagacca
     2281 gcccacaccc agaccaatgc gtaatccggt gaggatttcc ggcagcgcgc cgggcaaaat
     2341 gacaaaccac agcacctgcg cacggctggc acccagcgac tgggcggcac gaatgcgaac
     2401 ctgctgcgcg cttttcaccc ccgccagcgc cgccaccgtc caccatacag tgagtaaaac
     2461 cgctaacgtg ccaatgctta aggtcacctg acgcgagagc ggccagcgcc atttcagccg
     2521 ccgcgaatgc agtttttcat taatgagcac actcatgaga acgcctcccg ttgctcaaat
     2581 acgcggctta aaacatattc gcgcatggcg atgaattgtg gatcggactt gatgctgcgg
     2641 ctcgactctc ccgcaacaaa gcggcgagca aagttgagcg gcagccgctc cagcacacgg
     2701 ccagggccgg atgaaagcag aaccagttca gtcgccataa acaccgcttc ttctatatcg
     2761 tgggtaatca acagcacctg cttgcccgtc tcctgccaga gtttcagcag cagagtttgc
     2821 atctggtcgc gggtgaaggc gtccagcgca ccaaacggtt cgtcgagtaa taacagctgg
     2881 ggattcgccg ccagcgcgcg ggcaatcccc acccgctgac gttgaccacc ggaaagctgc
     2941 cagatatagc gtttttctgc gccttccagc cccacttttt tcagcatctg gtgcgcgatt
     3001 tccagtcgct gcattttctc tatacctgcc aattgcaggc cgaacgccac gttgtcctgt
     3061 acattgcgcc acggtagcag cccttcattc tgaaaaacca ccccgcgctc tgctcccggt
     3121 ccctcaatac gcttacccgc cagttgaatg ctgccatgct gataaggcac aaaaccggca
     3181 atcagattca gcagggtggt tttaccgcag ccggacggcc ccagcaccac cagtagctcg
     3241 ccgctttcca gcgtcaggtt gatatcttcc agtgccggtt tgccgccata atcggcatag
     3301 agatgagaga tttgcagcat ctgcgcctcc ttttattgca cgaagcgcga ggtaacgtac
     3361 tggctgtaat cattcgctac agccgggacc ttgccctgct ctttcaaaaa ctgcgcggtg
     3421 tcgatgattg ctttgttcac cggtccggtc agttctgccg tttgttgctg cggcgtcaga
     3481 taggtatttc ccttcaccag ccccggaacg tcaccttcag gcacgccgct taaacgcgcc
     3541 agtttgctga tgttttccgg ctgtttcagc cacgcgtctg ggttagcaat gtacggttgc
     3601 tgagcatcga tggcgctttt agcgaacgct ttcacgacct caggatgttt ctcggcaaaa
     3661 tctttgcgca ccacccagac gtccagcgtt ggcgcacccc actgcccgac ctgttcagaa
     3721 tcggtcagca ccttgccgtc tttttccagg gcgttaaccg ccggtgccca gacataagca
     3781 ccatcaatat caccccgctg ccatgcggcg ataatcgcgg gcggctgcag gttcacaatc
     3841 tccacttgcc ctggtttaat cccccagtgt ttcagcgccg ccagcaggct gtagtgggtg
     3901 gtggagataa acggtacggc aatgcgtttg ccaatcaaat cttcgggttt gctgatagtt
     3961 ttctttacca ccagcgcttc ggagttaccc agttttgacg ccagcaagaa gacttcaatc
     4021 ggcacctgtt ggctggctgc aaccgctaat gggctggaac cgaggttgcc gatttgcacg
     4081 tcgccagaag ccagcgcccg cacgatgctg gctccgctgt caaacttacg ccagtccacg
     4141 ctcgccccgc tctctttagc aaaggtgttg tcggcctgag ccactttcgc cggttcggct
     4201 gaggtttgat acgccacggt gacgttcacc gcctgtgcct gaaaagcgat gaatgccagt
     4261 gcggcaagaa gtgtgttacg cgatgaaatt gccatgattg tctgctcccc tgtcttgtta
     4321 tgggagcagt attcaggaat aaaaacattc attaaaagaa ttagtcgtta tcgcacagat
     4381 gattttattc ttagcaaaaa aacggcaaag gaaagattaa atatgcaatt actccgttct
     4441 aacaattggg aaaattacta cacttcattt t
//
LOCUS       DAZKHC020000207         4457 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZKHC020000207 DAZKHC020000000
VERSION     DAZKHC020000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.208"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(9..248)
                     /gene="yeiS"
                     /locus_tag="ACHOSM_RS23745"
                     /old_locus_tag="pgaptmp_004749"
     CDS             complement(9..248)
                     /gene="yeiS"
                     /locus_tag="ACHOSM_RS23745"
                     /old_locus_tag="pgaptmp_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HGU4561085.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(251..970)
                     /gene="sanA"
                     /locus_tag="ACHOSM_RS23750"
                     /old_locus_tag="pgaptmp_004750"
     CDS             complement(251..970)
                     /gene="sanA"
                     /locus_tag="ACHOSM_RS23750"
                     /old_locus_tag="pgaptmp_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HGU4561086.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1120..2004)
                     /gene="cdd"
                     /locus_tag="ACHOSM_RS23755"
                     /old_locus_tag="pgaptmp_004751"
     CDS             complement(1120..2004)
                     /gene="cdd"
                     /locus_tag="ACHOSM_RS23755"
                     /old_locus_tag="pgaptmp_004751"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGU4561087.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDELDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2134..2829)
                     /gene="yohK"
                     /locus_tag="ACHOSM_RS23760"
                     /old_locus_tag="pgaptmp_004752"
     CDS             complement(2134..2829)
                     /gene="yohK"
                     /locus_tag="ACHOSM_RS23760"
                     /old_locus_tag="pgaptmp_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HGU4561088.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2826..3224)
                     /gene="yohJ"
                     /locus_tag="ACHOSM_RS23765"
                     /old_locus_tag="pgaptmp_004753"
     CDS             complement(2826..3224)
                     /gene="yohJ"
                     /locus_tag="ACHOSM_RS23765"
                     /old_locus_tag="pgaptmp_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HGU4561089.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            3463..4413
                     /gene="dusC"
                     /locus_tag="ACHOSM_RS23770"
                     /old_locus_tag="pgaptmp_004754"
     CDS             3463..4413
                     /gene="dusC"
                     /locus_tag="ACHOSM_RS23770"
                     /old_locus_tag="pgaptmp_004754"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HGU4561090.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRIPWPEVVALLQKYTCLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
BASE COUNT         1198 a         1127 c         1147 g          985 t
ORIGIN      
        1 agaaaatatt aacgatagaa aataagataa acaatcatgc cgatagataa aattccgaag
       61 ccgatatatg ccaccatata agcgaaaaag agtccgggat tttttgctcg ccagacatac
      121 accggttccg gtgcattttt aacccgttta tcaatcgcgc ctgcgcgcca gcctttccat
      181 gcttcgcgga aacaaaatat cggaataagg aaaaaaacgg caacgacaaa aaactgctgt
      241 acatccataa ctactttcct tgtttctttt gtaattcaag taactgttcg ggtgtgacgg
      301 cgggataccc ctgcgcatct tccggcacct ggtgcatagc cggaataggg accagcggcc
      361 ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc cagcgcaccg aaacgggcgg
      421 caaattcgcg aatacgtaca gacagcatat ctttcggtga cggtacggca taacactgag
      481 cctgaatccc catatgcagc gcaataaata atgcccgctc acagtggaaa cgttgggtga
      541 taataatgaa atcattagta tcgaaaactt tgcgtgtacg cacgatggaa tccagcgtac
      601 gaaagcctgc gtaatcgaga acaatatctg atgggtcgac accagcagcg attaaatctt
      661 tgcgcatggt catcggctca ttataacttt gcaaagcgtt atcgccgctc agtaatagat
      721 aatttacctt accgctgtta taggcattaa tcgctccttg aatgcggtag cgataatact
      781 gattaattac gccagtacga taatattttg ctgtgccgag caccacgccg acctggcggt
      841 aggggagatc ctgcaattcg tcgtagatat aaggcgcggt tttccagctc atccagcgat
      901 cgaggccgag cacagtcaac agcagcaagc cgatcaggac taacaggctg aggaacacgc
      961 gctttaacat gcaggagact cgatctaagg aaaatttcca tcaggctact ttacccgctg
     1021 gttttgcgca agaaacggca aactgtttgc cgggaatttc gacaccagga gagggctggc
     1081 aagccggaca aggcgttcac gccgcatccg gcaccagtct taagcgagaa gcacgcggtc
     1141 gatactgtga cagccgagag ctttcagcgt tgcggaggtg gcatcccact gaatcaacgg
     1201 cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc gggtaatcat aacccttgag
     1261 attcaacaga attaacgctc cctgcaacgg tggcagagtc gggttgaatg cggcgttttc
     1321 agcgtagctg ccactgaaaa tacgaccgtc tttacattcc agcgcgacac cgcttggcga
     1381 cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc gccgcctgag aaagcgcatc
     1441 accagtcagc gcatagccgt gatccagctc gtccatcagc agcgttttaa tctccagatc
     1501 tttcggccca aaggcatccg gcagatagtc acgcagcgcg tgtgcctcgc ggcccggcaa
     1561 atgaatacgc agatccagac cgctgttcag ttcattcata aactgacggc agtgaccaca
     1621 aggcgtgtag ttaacggtga tggctgcaag cgctttttca ccactcaacc aggcgtggct
     1681 gatcgcgctt tgttcggcat gaacggtttg ctgcattgtc gcaccaataa attccatatt
     1741 ggcaccgaaa taccaggttc cgctcacacc gcgcgcaatt gcgccaacat taaaattcga
     1801 caatggcgta cgcgcacagg ccgccgccag cggaagtagt gcgaatgcca gcgcgtcttc
     1861 gtccagcccc gttgcgctct tcagcgatga gacttgctcc ccggtcaaca aagcggggaa
     1921 gtacttgtct gccagaatag gttccagtgc agattgcaag ttatccgcaa gttgggcaaa
     1981 agcggtttga aaacgtggat gcatggcgtt gcctcataat catgtaatgg gataacagtt
     2041 tacgaacccg ttatggcttt atatgtgatc tgaatctcat taattatgca acacgtgaaa
     2101 cttacatcaa aaatgcgcga cgtatcgcaa attttagccc attactgcca gaataatcgg
     2161 gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat aacaccagcg ccagcgaact
     2221 aaatgcacct tcctgataat ccagctcggc gcagcgcgcc gtaccgaggg cgtgcgaggc
     2281 tgtccccatt gccagaccgc gcgcagcttt ggtacgaata cgcatcgcat taagcaatgt
     2341 atggccaaat accgcgccga ggatgccgac gaaaatcacg caaactgcgc tgattgccgg
     2401 aataccgcca atactgccgc caaccgccat tgcaataggc gtggtgactg atttcggcag
     2461 gattgacgcg gcgatttccg gtgaagcgcc catcaacaat gccacggaag tccccgtcac
     2521 catcgcaacc acgctgccga taaaacagat ggtgatgatc gatttccagc gcgcgcggat
     2581 ctggtgtagc tgctcatata aaggataggc cagcgcgacg accgccggtt gcagcagatc
     2641 gtttagcact tcgctacctt taaagtaact gtcgtaagag atgccggtca gcattaaaaa
     2701 aggaatgatc accaccattg ctaccagcaa cgggttaagc aggggaaact tataccgtgc
     2761 cgccagtttg cgggcggcaa aaaagacaat caacgtcagc ggtaatgacc accagatatt
     2821 cgccatcatt cttctgatcc tttctgacct acgactttac gttcgccgtg taccagttgc
     2881 gaactccagc tcaccaccag aaaaaccacc agcgtactga ctgcacagga aaccactacc
     2941 gggccaaact gtgcgcggag caaatcaaaa tattgcatga cgcctacgcc aatcggcaca
     3001 aacaatagcg ccatatagcg aatcagtacg tagcaccccg gattgaccca ttttgccgga
     3061 agaatttgca aggccagcag gacaaacagg atcagcatcc cgatgatgct gcccggaatg
     3121 gttaccggta gcagggaagc aatgaaaatg cctgcataca ggcaggcata aatcaggacg
     3181 aaagcgcgta aatattgcca gataatgttc agtgtcttac tcataatcaa caccccagat
     3241 gagatggatt catcatacaa ttaattcgta aaacgtgcta ccgatcacat taagaaatca
     3301 gcgcttattt tttcgctccc cggcattacg caatgcgcaa cgaactcagg catacatcaa
     3361 gcatagagag ggattacagt ttgcagcaat ccctctttgc agacctttaa tccgttgacc
     3421 ataggcgcta acatggcgcc tcattttttt gcgggtgaag atatgcgtgt gttactggct
     3481 ccgatggagg gtgtgcttga ctctctggtg cgtgaattgc tgaccgaagt taacgactac
     3541 gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac tgctgccggt aaaagttttt
     3601 catcgcattt gccctgagct acaaaacgcc agccggacac catccggtac gctggtgcgc
     3661 gtgcagttgt tgggtcagtt cccacaatgg ctggcagaga acgccgcccg tgcggtggaa
     3721 ttaggttcct ggggcgtgga tctcaattgc ggctgcccgt cgaaaacggt caacggtagc
     3781 ggtggtgggg cgacgttact caaagatcct gaactaatct accagggtgc aaaagcgatg
     3841 cgtgaagctg taccggcgca tttgcccgtc agcgtgaaag tgcgtctggg ctgggacagc
     3901 ggtgagaaga agtttgaaat cgccgatgcg gttcaacagg ctggtgctac ggagctggtg
     3961 gtgcatggac ggacaaaaga gcagggttac cgcgcggagc atattgactg gcaggcgatt
     4021 ggcgagattc gccagcggct gaatattccg gtgattgcca acggcgaaat ctgggactgg
     4081 cagagtgcgc aacaatgcat ggcgatcagc ggctgcgacg cagtgatgat tggtcgcggg
     4141 gcgctcaata ttcccaacct gagccgggtg gtaaaatata acgaaccgcg aataccgtgg
     4201 ccggaggtgg ttgctttgct gcaaaaatat acctgtctgg aaaagcaggg cgataccggg
     4261 ttatatcacg ttgcgcggat taaacagtgg ttgagttatt tgcgtaaaga atacgatgaa
     4321 gcaacggaat tatttcagca tgttcgggtg ttgaataatt cccctgatat tgcaagggct
     4381 attcaggcaa ttgatatcga taagctgcga taatagtgga atcgcagttt atcagagaca
     4441 atgaatcgct tattcag
//
LOCUS       DAZKHC020000208         4346 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZKHC020000208 DAZKHC020000000
VERSION     DAZKHC020000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.209"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..4085
                     /gene="espI"
                     /locus_tag="ACHOSM_RS23775"
                     /old_locus_tag="pgaptmp_004755"
     CDS             3..4085
                     /gene="espI"
                     /locus_tag="ACHOSM_RS23775"
                     /old_locus_tag="pgaptmp_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspI"
                     /protein_id="HGU4561091.1"
                     /translation="MYALKYSSLTGGLIAVSELSKKVTGKTGRRLMTVSLVLSVTLST
                     LPGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIEINDNNGNKVGVLDVPMLDFSSLS
                     RDGHTTLIHPGYVVSAKHGGLQSVSSATFGYDQIYKIVDNNLAGIDFSAPRLNKLVTE
                     VIPADIQGKDKFNNNRYTAFYRAGVGSQYIRYANGTDKLLQAYTPEKAYLTGGTVGKP
                     YYTHYNGMKMISANPGNTFDKNQGPLASYGQSGDSGSPLYAWDNIDKKWVLAGVTLHN
                     YGVKGARNDWLLIPHDFISQKLQDDLKPIIVASPEENILRWEFDRSRGTGTLSQGEKI
                     FSMTGSVNGNANTGNNLVFSGNEGKIELVSSVEQGAGYLQFDKDYTVLTNNNSTWTGA
                     GIIVGDEANVKWGVNGIAGDNLHKVGSGTLTVNGHGENKGGLKVGDGVVVLEQQPDAN
                     QKQQAFSHINIASGRATVKLNGANQVDADNISWGYRGGKLDLNGYDFTFSRLQAADYG
                     AEISNDNQTDKSIVTLSLSPLKAEEINVVVNNINIMGGTGKPGDLYYTTFDGNYYLLK
                     SNRYGSALFGALNNQSEWQRLGKDKEKAIGLYTQMKMQESAPLSYIYHGKITGNTSVE
                     IPKLAGNDILTLDGSVSISGDMSKQDGALIFQGHPVIHAGQTVSASQSDWENREFSLN
                     NLNLNNVDFSLSRNAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKSAAS
                     GTSSYTGHITLEQKSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLNIHK
                     GGALTAQGGLFTSGSIDIGDASLLLTGTPVNSDDAAFLPTINMADGGFKLMSDSSVLK
                     ARDQASVVGDIISDKQATISFGTESGKEGILSEKASRGLAVGLLSGFNTAYRGAIHAP
                     SASATMNNTWWQLTGDSSLRSLKNTGSMTYFTGSAANKAFHTLTVDELTTNGTAYAMR
                     TDLKNADKLVVNQKLSGKDNILLVDFLNKPTGEKLDIELVSAPGNSSKDVFKGSEQEI
                     GFSNVTPVITAIDAGDKTTWNLTGYRMAENPAATQSASGLASVGYKSFLSEVNNLNKR
                     MGDLRDINGEAGAWARIMSGTGSAGGGFSDNHTHVQVGVDKKHELDGLDLFTGFTVTH
                     TDSSASADAFKGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDY
                     STHSWYAGAEAGYRCHVTEDTWIEPQAELVYGAVSGKQFAWKDQGMHLSMKDRDYNPL
                     IGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLM
                     SVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
BASE COUNT         1263 a          883 c         1067 g         1133 t
ORIGIN      
        1 aaatatacgc gcttaaatat agctccctta ctggtgggct tatagctgtg tcagaattaa
       61 gtaagaaggt cacaggaaaa accggcagaa gattaatgac ggtttccctg gtattatcag
      121 tgactctttc tactttaccg ggtaaagcat caacggtcag cgcagaaata ccatatcaga
      181 cttttcgtga ctttgctgaa aataaaggtg tgtttacccc cggagtgaca ggaattgaaa
      241 taaacgacaa caatggaaat aaagttgggg ttcttgatgt tcccatgctt gatttttcca
      301 gtctttctcg tgatggtcat accacattga ttcatcctgg ttatgttgta tcggctaaac
      361 atggtggttt acaaagtgtt tcatcagcaa cttttggtta tgaccagata tataaaatag
      421 ttgataataa ccttgctggt atagattttt ctgccccacg attaaataag cttgttacag
      481 aagtaattcc cgcagatata cagggaaagg ataaatttaa taataaccgg tatacggctt
      541 tttaccgtgc gggcgttggc tctcaatata tccgttatgc aaatggcaca gataaactac
      601 tgcaggctta cactccagaa aaggcttatc tgaccggcgg aacagtgggg aaaccttatt
      661 atactcacta taacggtatg aagatgattt cagcaaaccc gggaaatacc tttgataaaa
      721 accagggacc tcttgccagt tatggacaga gtggagacag tggttcgccg ttatatgcct
      781 gggataacat tgacaaaaaa tgggtattag ctggagttac tctgcataat tatggagtaa
      841 aaggtgcacg aaatgactgg ctcctgatac ctcatgactt tatcagtcaa aaattacagg
      901 atgaccttaa accaattatt gttgcttctc cggaggagaa tatcttacgc tgggaatttg
      961 atcgttccag aggtacaggt actctcagtc agggagagaa gattttttcc atgactggta
     1021 gtgtaaacgg aaatgcaaat accgggaaca atcttgtgtt ctcaggtaat gaagggaaaa
     1081 tcgaactggt atccagtgtg gaacagggag ccggatatct tcagtttgat aaagactaca
     1141 ctgtactgac aaataataac agtacatgga ctggtgccgg aattattgtc ggtgacgagg
     1201 caaatgtcaa atggggagtt aatggcattg ccggtgataa tctgcataaa gttggttccg
     1261 ggacattaac tgttaatggt catggtgaga ataaaggtgg ccttaaagtt ggggacggcg
     1321 ttgttgttct tgagcaacag ccagatgcaa accagaaaca acaggcgttt agccatatca
     1381 atatagccag tggtcgggca acagttaaac ttaacggggc aaaccaggta gatgcagata
     1441 atatcagctg gggatatcgg ggtgggaaac tggatttaaa tgggtatgat tttacctttt
     1501 cccgtcttca ggctgcagat tatggtgctg aaatcagcaa cgataatcag acagataaat
     1561 ccatagtcac actttcgtta tctcctctga aagcagaaga aataaatgtg gttgttaata
     1621 atataaatat aatggggggg acaggcaaac caggtgatct gtattatacg acctttgacg
     1681 gaaattatta tctgttgaaa agtaaccgat atggcagcgc tttgtttggc gcgctgaata
     1741 atcagagcga atggcaaagg ctgggtaagg ataaagaaaa agcaattggg ttatatactc
     1801 agatgaaaat gcaggaaagc gctcctttat catatatata tcatggaaaa ataaccggta
     1861 ataccagtgt ggaaatcccc aaactggcag gcaatgatat tttaacgctt gatggctctg
     1921 tcagtatatc aggagatatg tcaaaacagg acggtgctct tatcttccag ggacaccctg
     1981 ttattcatgc agggcaaact gtttctgcat cgcagagtga ctgggagaac agggagttct
     2041 cactcaacaa tctgaatctt aataatgtgg acttcagtct gtcccgtaat gcatttatga
     2101 acgggaatat cagggccgtt aaccagagca ctgttattat cggcggagat acagtcttta
     2161 ctgataaaaa tgacggaaca ggtaatgatg tcatcagtgt tgaagggaaa tctgctgcct
     2221 caggaacatc ctcctataca gggcatatca ctctggagca aaaatcagca ctggatatcc
     2281 gcgataattt tcgtggcggg gttacgtctg aagacagtca tatcaatgtt tcttcatctt
     2341 cagtcctgtt ctcagatgca tcgtcattta taaacagctc cctgaatatt cataaaggag
     2401 gtgcgctgac cgctcaggga gggctgttta caagtggaag cattgatatt ggtgacgctt
     2461 cccttctgct taccggtaca ccagtgaatt cagatgatgc tgctttttta ccgaccatca
     2521 atatggctga tggcggattt aaactgatgt ctgattcatc agtactgaaa gccagagacc
     2581 aggcatctgt tgttggtgat attatttctg ataaacaggc cacaatcagc ttcggaactg
     2641 aatcaggtaa agagggcata ttatctgaga aggcatcccg gggactcgcg gtaggattac
     2701 tgagtggttt taatacggca taccgcggtg caattcatgc cccgtcagca tctgccacta
     2761 tgaacaacac ctggtggcaa ctgacaggag actcctcact tcgctcgtta aaaaataccg
     2821 gaagcatgac atattttaca ggaagtgcag cgaataaagc attccataca ctgacggttg
     2881 atgagctgac gacgaatggc actgcgtatg ccatgcgtac ggacctgaaa aatgcggata
     2941 agctggtagt aaaccaaaag ctgtcaggta aggataatat tctgctggtt gattttctga
     3001 acaaacccac cggagaaaaa ctggatattg aactggtgag tgcaccgggg aacagcagta
     3061 aggatgtttt taaaggaagt gaacaggaaa taggtttcag caatgtcaca cctgttatta
     3121 cagctataga cgccggagat aaaacaacat ggaacctgac cgggtacagg atggcagaaa
     3181 atcctgccgc aacccaaagt gcctcaggcc ttgcatctgt ggggtacaaa tcatttttga
     3241 gtgaggtcaa caacctgaat aaacgtatgg gtgacctgcg tgacatcaat ggtgaagctg
     3301 gcgcatgggc acgtatcatg agcggaaccg gctctgccgg tggtggtttc agtgacaacc
     3361 acacacatgt tcaggtcggt gtcgacaaaa aacatgagct ggacggactg gatttgttta
     3421 ccggcttcac tgtcacacac actgacagca gtgcctccgc cgatgctttc aaaggtaaaa
     3481 caaaatctgt gggggccgga ctctatgctt ccgccatgtt tgattccggt gcctatatcg
     3541 acctgattgg taagtatgtt catcatgata atgagtacac cgcaaccttt gccggactcg
     3601 gaacccgtga ttacagtacg cattcatggt atgccggtgc tgaagcaggc taccgctgtc
     3661 atgtcactga ggatacctgg attgagccac aggcagaact ggtttacggt gctgtatccg
     3721 gtaaacagtt tgcatggaag gaccagggga tgcatctgtc catgaaggac agggactaca
     3781 atccgctgat tggtcgtacc ggtgtggatg tgggtaaatc cttctcaggt aaggactgga
     3841 aagtgacagc ccgtgccggt ctgggctacc agttcgacct gctggctaac ggcgaaaccg
     3901 tattgcggga cgcatcaggt gaaaaacgta tcaaaggtga aaaagacagc cgtatgctga
     3961 tgtccgttgg cctgaatgca gaaatcaggg acaacgtccg ctttggactg gagtttgaga
     4021 aatccgcctt tggtaagtac aacgttgata atgctgtcaa cgctaacttc cgttactcgt
     4081 tctgatactc cgtattcaaa agggcctgag ggccctttat gctcaggacc tgccgcccag
     4141 gtatacattt ccggggtatc agtcgatttg tttacttaac cgccatgacc aaagccggca
     4201 gcaatgtcgg ctttttacgt ccatatattc cactcatccg gaactgtgac acagaacctg
     4261 aatacaggat tgatcaccgg tgcccacaaa tggcacattt tcagtaagat tctgaatgtt
     4321 acattccccc ttcatgtgtg acacag
//
LOCUS       DAZKHC020000209         4287 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZKHC020000209 DAZKHC020000000
VERSION     DAZKHC020000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.210"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(494..1066)
                     /gene="hdeD"
                     /locus_tag="ACHOSM_RS23780"
                     /old_locus_tag="pgaptmp_004756"
     CDS             complement(494..1066)
                     /gene="hdeD"
                     /locus_tag="ACHOSM_RS23780"
                     /old_locus_tag="pgaptmp_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HGU4561092.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            1321..1653
                     /gene="hdeA"
                     /locus_tag="ACHOSM_RS23785"
                     /old_locus_tag="pgaptmp_004757"
     CDS             1321..1653
                     /gene="hdeA"
                     /locus_tag="ACHOSM_RS23785"
                     /old_locus_tag="pgaptmp_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HGU4561093.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            1769..2095
                     /gene="hdeB"
                     /locus_tag="ACHOSM_RS23790"
                     /old_locus_tag="pgaptmp_004758"
     CDS             1769..2095
                     /gene="hdeB"
                     /locus_tag="ACHOSM_RS23790"
                     /old_locus_tag="pgaptmp_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HGU4561094.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            2159..2806
                     /locus_tag="ACHOSM_RS23795"
                     /old_locus_tag="pgaptmp_004759"
     CDS             2159..2806
                     /locus_tag="ACHOSM_RS23795"
                     /old_locus_tag="pgaptmp_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGU4561095.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(2848..3378)
                     /locus_tag="ACHOSM_RS23800"
                     /old_locus_tag="pgaptmp_004760"
     CDS             complement(2848..3378)
                     /locus_tag="ACHOSM_RS23800"
                     /old_locus_tag="pgaptmp_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HGU4561096.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(3534..4100)
                     /gene="slp"
                     /locus_tag="ACHOSM_RS23805"
                     /old_locus_tag="pgaptmp_004761"
     CDS             complement(3534..4100)
                     /gene="slp"
                     /locus_tag="ACHOSM_RS23805"
                     /old_locus_tag="pgaptmp_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HGU4561097.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         1305 a          881 c          905 g         1196 t
ORIGIN      
        1 taaaatgtaa tccgatttaa atatcgagtc tccttgtttc gacttaagct ggcaattgga
       61 ttgccagctt tcttttaacg cattgaaatt acctgagtaa ataacgcatt catgtcttca
      121 ggtttttata tagcgtctgg agacacggat atttatgcaa tgtaatcaac gccagttgca
      181 aaaaaatgaa cccggattac ttatttaacc gcccatagca tcaacgctta cggaactctt
      241 tgttcacttt cccacagcac ttttaaaaca ttaaacctga ctacggaaaa tatcagccat
      301 gataatgttt gaaatggcta atttgccatg gagtgaaaaa aattagatga aattcagtag
      361 gttgaaataa tcactagcag gtaattattt caatgatagt gcgcaattga tctacaacac
      421 tgcgtagcgg agagagtatt aatcggatca tagtcacatc aagtgactat gatccgggtg
      481 acaaccggga taattattgc tgcttaacga acaaactggc gaagctgaac aggctggcgg
      541 cgctaaatat cagttcaatt cccaccagtg tggaaaccag cgttacagac accatcggcg
      601 ttgcaccaag gaatatccag gcaatgacga tatccagcac accaataacg agctgtagcc
      661 agctgccttt cattgaacgc tgacgatacc aactcatcag gcgaataacc cctgcaacac
      721 agaacaaacc ggcaataaat gccgcaatgg caaaaatgcc cagctccggt gcgcggatga
      781 agaaatagcc gatcaataaa taggcgactg cgacgaggaa accggataat accggccaga
      841 aattatgact gcggttgctg aataacccga caataagcgc aatacccgag cagattaata
      901 atgcacccac tactgtgctt aaaatatcgc cagagacgaa cgggaaactg atacacagca
      961 acccgacgat aaacagcagc acggcaataa actggattgc tctgcgatgc tttttcagca
     1021 tctccagatc aaacttcaaa attgttgcct tatctatata taacatagaa ccaccctata
     1081 aaattaagaa gaaaatcccc tgctatcaat ctatgccaaa aacgcgtcta agaatgcagt
     1141 cgatttaata aaaatttcct aattgtagta tctgatgcat ctgtaactca ttgtattgaa
     1201 ataaaaatat ctgattttga tattttccat caacatgaca tatacagaaa accaggttat
     1261 aacctcagtg tcgaaattga ttcgtgacgg ctctttcact ttatagttga ggatattacg
     1321 atgaaaaaag tattaggcgt tattcttggt ggtctgcttc ttctgccagt tgtgagcaat
     1381 gcagcggatg cgcaaaaagc agctgataac aaaaaaccgg tcaactcctg gacctgtgaa
     1441 gatttcctgg ctgtggacga atccttccag ccaactgcag ttggttttgc tgaagcgctg
     1501 aacaacaaag ataaaccaga agatgcggtt ttagatgttc agggtattgc aaccgtaacc
     1561 ccagctatcg ttcaggcttg tactcaggat aaacaagcca actttaaaga taaagttaaa
     1621 ggcgaatggg acaaaattaa gaaagatatg taattccggg aatgcgttac atcgtacttc
     1681 cttgcatatt gaacaggccg gaatatcttc tttaaaagca gctattcctc ctgttcatat
     1741 ataatctcta tattgaatgg gttacaaaat gaatatttca tctctccgta aagcgtttat
     1801 ttttatgggc gctgtagcgg ctttgtcact ggtgaacgca caatctgcgc tggcagccaa
     1861 tgaatccgct aaagatatga cctgccagga atttattgat ctgaatccaa aagcaatgac
     1921 cccggttgca tggtggatgc tgcatgaaga aacagtatat aaaggtggcg ataccgttac
     1981 tttaaatgaa accgatctca ctcaaattcc taaagtgatc gaatactgta agaaaaaccc
     2041 gcagaaaaat ttgtatacct tcaaaaatca agcatctaat gacttgccga attaatgagg
     2101 tacaagtaaa aaggagtagc aagttgagcc atcttgctgc tcctttttgc atttttatat
     2161 gacagcagaa tttattatac gtcttatact cgcggcaatt gcctgtggcg ctattggcat
     2221 ggaaaggcaa atgcgcggca aaggagcagg gttacgcaca catgtattaa ttggcatggg
     2281 aagcgccctg tttatgattg tttcgaaata tggttttgct gacgtgctgt ctttagatca
     2341 cgtcggactc gaccccagcc gtattgctgc tcaggtggtg acgggcgtcg ggtttatcgg
     2401 tgcaggtaac attctcgttc gtaaccaaaa tattgtaggt ctgacgacgg cagcggatat
     2461 ctgggtgacc gccgccatag gtatggttat tggcagcggt atgtacgaac tgggtattta
     2521 tggttcagtg atgaccttgt tggtgctgga agtcttccat caattaacct tccgcctgat
     2581 gaacaaaaat taccatttac agctgacatt agtgaacggt aatacggtat caatgctcga
     2641 ctggtttaag cagcaaaaaa taaaaacgga tttagtttcg ttgcaggaaa atgaagatca
     2701 tgaagtggtc gccattgata tccagcttca tgccacaacg tcgatagaag acctgttgcg
     2761 attattaaaa ggtatggcag gggtgaaagg cgtttcaatt agctaatctg tgtggcagcg
     2821 tagccagact caccgtaagc ctgaaattca caccagataa tcaatatgct gatggcgtac
     2881 cagctcattg atccgcttaa gattgaggat catcatgata ttgtgtttat ggcagtagaa
     2941 cgtttttagc gatattttca gtttctccag aatatcttcc gttgtcattt gattcgcaat
     3001 gcagctcaga acctgatgtt gcttgcgcga aagtgacaca gccgcttcag gcacagagca
     3061 gtaactgcca ttgatgagtg cgacaaaatc aatgatagtg atagtccgcg gaacaaaggt
     3121 caccggcact ttccccaggc agcgtttaat attaaagggg gagaaaataa tcacatggac
     3181 aggttttaat cgttcaattt gagtgagaaa attagaatga aatacattat tcattaatgt
     3241 atcaataatg acgaaggcat tctgatgcaa cattacgtcg atctcttctt cgttctgtat
     3301 atgaacgaca ttacctttac tcagaatgtt tttcatcgcg gtgaagaaca tcgtatccct
     3361 ggtaattata agaaacataa ccgactcctg gtgcggacat taactttata atcagtttgc
     3421 caaaggtaat taacaggaca gggcttataa taataatagt tagcttactg tatattaatt
     3481 ttatgtaata catgatgcag atgctgagaa agcatctgca tctttcggtg gtgttatttg
     3541 accagctcag gtgttacctg actcaccgca ttggtgtagt aaggcgcacc ccagcccggt
     3601 tccggccaga atgcaccata gccgtaatcc cacaggttat aggtggtatt aaccacttct
     3661 ctcaaatgcc acacctggat gccctgcata ttcacttcca ggaagttata cgggacttta
     3721 ttgataaagc ctggttgttc accctgaatg gtaccgagga tggtaacaaa gtgattacga
     3781 tagttcactg gatcaaggaa gccgctttgt ctggcgagca gtcggccctg atagttggct
     3841 tcaatatcag gcttcgcata gctatccaac ggtaatacag cgatttctaa caacgtatcc
     3901 gttttgccat tgataacgtt gataaccttc ccaccaaagc gcgcttgttg accaacatat
     3961 aaccccggct ggttatgaac agcaacaaaa cttttttgaa tatcaggttg gttattgcct
     4021 ttgatatttt gcggaattga actacatgcg gcaagcaaaa atgaaaggct gaggatgagt
     4081 gcaccttttg tcatgttcat gttactatcc ttatcaacaa atattaaaac cataatagat
     4141 atttctttat agttttcatc tgattctgag gtgatatgcc tgatagtaac gtaaacctgt
     4201 ctgatgttta tatcccttct gtttagttca attccgttat tttggggcgt ggtatgagta
     4261 tccatcgccc aataatataa ccctttg
//
LOCUS       DAZKHC020000210         4168 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZKHC020000210 DAZKHC020000000
VERSION     DAZKHC020000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.211"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..283
                     /locus_tag="ACHOSM_RS23810"
                     /old_locus_tag="pgaptmp_004762"
     CDS             <1..283
                     /locus_tag="ACHOSM_RS23810"
                     /old_locus_tag="pgaptmp_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4561098.1"
                     /translation="TVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPL
                     KVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            464..1108
                     /locus_tag="ACHOSM_RS23815"
                     /old_locus_tag="pgaptmp_004763"
     CDS             464..1108
                     /locus_tag="ACHOSM_RS23815"
                     /old_locus_tag="pgaptmp_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HGU4561099.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            1195..1503
                     /locus_tag="ACHOSM_RS23820"
                     /old_locus_tag="pgaptmp_004764"
     CDS             1195..1503
                     /locus_tag="ACHOSM_RS23820"
                     /old_locus_tag="pgaptmp_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HGU4561100.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVTARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGVIGQ
                     "
     gene            1917..2897
                     /locus_tag="ACHOSM_RS23825"
                     /old_locus_tag="pgaptmp_004765"
     CDS             1917..2897
                     /locus_tag="ACHOSM_RS23825"
                     /old_locus_tag="pgaptmp_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HGU4561101.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVAVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            2890..3306
                     /locus_tag="ACHOSM_RS23830"
                     /old_locus_tag="pgaptmp_004766"
     CDS             2890..3306
                     /locus_tag="ACHOSM_RS23830"
                     /old_locus_tag="pgaptmp_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGU4561102.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKLSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(3308..4168)
                     /gene="umuC"
                     /locus_tag="ACHOSM_RS23835"
                     /old_locus_tag="pgaptmp_004767"
                     /pseudo
     CDS             complement(3308..4168)
                     /gene="umuC"
                     /locus_tag="ACHOSM_RS23835"
                     /old_locus_tag="pgaptmp_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
BASE COUNT         1074 a          887 c          976 g         1231 t
ORIGIN      
        1 aacggtcagg acctggattg gggaggcggt tgccgccgct gctgctgacg gtgtgacgtt
       61 ctctgttccg gtcacaccac atacgttccg ccattcctat gcgatgcaca tgctgtatgc
      121 cggtataccg ctgaaggtcc tgcagagcct gatggggcat aagtccatca gctcaacgga
      181 ggtctacacg aaggtgtttg cactggatgt ggctgcacgg caccgggtgc agttttcgat
      241 gcctgagtcc gatgcggtgt ctatgctcaa aagaattcct taagctctga cttttttaaa
      301 tccctttcgg atatctcatg gatatattat cggtatttca tattctgttg acatgcagga
      361 ccggtataga gtaaaaaaaa catctattga gtacctcttg gatatctgaa aggtgtcttt
      421 ggggttcttt atgggtatta catgtggatt ctggagatac tctatgcctg ttattgctaa
      481 tacgcatccc aaaggtggtg tgggtaagac aacttcatcc gtaaacattg ttggtgaaat
      541 gaagtctgat acagttgatc tggatactca taccgggctt tctatcattc tggggctgag
      601 acctgaagga aaagaaattt ccgtgaaagt acccaaaaca gtggatgagt taatcgaaat
      661 tatgactccc tacaagaaca gcgataagac gttacttatt gactgtgggg gatttgactc
      721 agatcttacc cgtactgcga ttgcctttgc agactgcgtt attgtccctt caaaagactc
      781 cctgactgaa cgtattggtt tgatgcattt tgatggagta ctggacgaaa tcagctctat
      841 catggggacc gatataactg ctcacctgta tctctgtaaa gttaatccta acaagaagaa
      901 attccctaag cttgatgcaa tcctgccatc gttcaaacat ctgaagctga tgaagagccg
      961 catttctgct cgtgcagaat ttgaggatgt cattgagact gggatgggaa tcactgagtc
     1021 tgtgcatggg cgctattcgg caggaggtaa ggaagttatt gccctgatcg aagaaatcaa
     1081 tcatctgatt gaaaataaca aacagtaatg tgtatctatt gggtatccat atagtattct
     1141 ttgggctttt aatggatatc ttttaagtat ctaacaaaca tcctgggtta tctcatgagc
     1201 aaagcgaaat ttagtgcaca ggctgaaaaa ctgaaaaacc tggtggattc tgccaggtta
     1261 caaacttctt cttctgccgt aacagcaaca gccccgtctc agccaaatga aggtgcagag
     1321 gaagtttcag gtcgtcccgt gactgccagg acgaaggcta agaatcttaa ggccataccg
     1381 ctgtcgtatt ttgaagctca tgcggaactt aaagcgacca gtaaaacatc tctggatttt
     1441 agctcatata ttattgaggc tataagagag aagcttgaac gtgatggtgt aataggtcaa
     1501 taaactatcg gataccttaa gggtatctga tgtgtgtctt ttaaatgcgt tatggggatt
     1561 cttcacgtac ctaaagagtc cccattagac atctctctgt ttatggtgac atgattcgtc
     1621 accgctttcc tttcctctca gtttcaccat ttcattcctt ttcccttaac ctgaaccatg
     1681 atgtcaaaga tatttttata ttgcatttat gtatcacata taaccattca taatggttct
     1741 gtatctatga ttttctctgg tcttaaagtt atgtgtacaa tgtacatgca gtttatcgtg
     1801 tttttatgtt gcatttgtcc tttcagaaag accattttcc attataattg aatatacatt
     1861 gcatgtatga ggcatatttg ttttttctat ttgtttatga gcaacggaga ctggttatga
     1921 aaatctttat tgatgatggt tctaccaaca ttaaacttgc ctggttggag gatggtgacg
     1981 tgaaaacgtt aatcagccct aacagcttta aaccggaatg gtcttttagt ttactggatg
     2041 atgctgcccc ggctaattac gagatcgacg gcgagaagtt ttcatttgat ccattaagtg
     2101 ctgatgctgt cgtaacgact gaaacacgct atcagtacag tgatgtgaat gttgtggcca
     2161 ttcagcatgc gctacagcag accggcctga aagcgcagcc tgttgatgtc atcgtcacat
     2221 tgccgatctc tgaatacctt gacgccaaca atcagaaaaa taagcaaaac attgaaagaa
     2281 agaaaaaaaa tgtgatgcgt gaagtacggg tgcaggggag tgatgcattt gttattcgca
     2341 gtgttgccgt tcttcctgaa agtattcccg ctggtttcag tgttcttgcg gggcttgagg
     2401 atgatgaatc ccttttgatc gtagatctcg gtggtacaac tcttgatgtg tcgcatgttc
     2461 gtagcaaaat gacagggatt acaaaaacct ggtgtgatcc gaatatcggc gtgtccctga
     2521 ttacatccgg ggttaaggaa cagatggctg tacatgctaa tacaagagtg agctcattcc
     2581 aggctgacaa tatcattgtg caccgtaatg aacctgacta cctttcccgt cgcatttata
     2641 atgctgaaca gcgtgagtct atcattaatg ttatcaatga acggcagaag ttgcttatta
     2701 aacgagttaa tgatgtcatc agccgtttca ccgactatac acatgtaatg tgtgttggtg
     2761 gtggggctga aattgtggcc gaggctgtga agaacctgac taaggttcct gatgagcgct
     2821 tttatctgag ctcatccccg cagtttgatt tagtcatggg aatgataaaa atgaaaggtg
     2881 gtgtgactaa tgagtgatga aaataaatcc agacgctgct catttgaact tttcccggat
     2941 gaacggacag gagataagat agcagatgag ctcattgcaa atgaaaaact gaaggagcgg
     3001 ggacgtttca tgcgcgcgat gttagttact ggtgcggcgt ttgcagctat agataaacgt
     3061 cttcctttgc tgatatcgga gttactgaca gaaaatacga ctctggatga tattaataag
     3121 gtgatttcca gcgtaatacc aggtgctttt tcggttgaaa agaaattgct tgaattactg
     3181 gaaaaactat ctggtctcca tacgtctgtg gattgcagta ctcctcttac agagcagagt
     3241 ctgtcgagaa atgacggtga agaccagacc agaagaaacg ctgaaaatat gttcggagat
     3301 gactgaacta acccagcctg gctaggggaa tatctcgcca tttagttgta tcacactgac
     3361 tcaacatttc ccgtttcatt tgccattcag gggcggttcc tcgccccgca aaccacactt
     3421 tctcttttcc cgactggttc agttcatcga gtgttttcat taacttttca ctgtttttac
     3481 ggggctggat ttcatcaaat aatcccggct gcgctatacc cgatggtgtg aaatcagcca
     3541 gcatgactcc tgccttcata tagcggtacc cttcacgcca gacatggttt aaggctctgc
     3601 atgcggcggc aataatgtcc cggctgtcct gtgtggcaat gaaagctttt ccacagcggc
     3661 attgctgtaa cagggttctt ttactgcaaa gggggatgtc cgtacaaatg tcgtcacctg
     3721 ccgacaatac tgacgctccc cacgtagttt ctctgcggcc cgctcttcat actgaacaac
     3781 agcctggtgc atggcatctt tgtctgtgat tcgttcacca aaactgcgac tacagacaat
     3841 ctgctgtttt gccggtggtg cttcttccag ggatatgctg gacttgccgt tgagttcgcg
     3901 taccgtacgc tcaagaatga cgctgaagtt tttccggatg aatgccgtgt tagcctgcgc
     3961 cagttgcagt gctgtgttta tacccagcgc attcagcttt tccgtcagtc tgcgtcctac
     4021 tccccagacc tcaccaactg gctgcagccc cagtagcttc aagatccgat tacggttttc
     4081 tgccgtcagc gcgaccacac cggaaaactg tggccattgc tttgttgccc actgtgcact
     4141 tttagccagc gtttttgtag gcgcaatg
//
LOCUS       DAZKHC020000211         4097 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZKHC020000211 DAZKHC020000000
VERSION     DAZKHC020000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.212"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..2803
                     /locus_tag="ACHOSM_RS23840"
                     /old_locus_tag="pgaptmp_004768"
     CDS             <1..2803
                     /locus_tag="ACHOSM_RS23840"
                     /old_locus_tag="pgaptmp_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HGU4561103.1"
                     /translation="ELNPLAMAHPLKSFQYASVGTEHKLFDSEKLNIQPDNIEQVISK
                     GKALQKDFFDSYIENKETLPIITQSDGVHYKKIALNGLLLASGIFKPLNNNYKPQSGH
                     GNLFDEIHIRHSRSLPEEHAAPAEKLGVLIAKKLEESIVPKFIPFYNILTKLEQEQEQ
                     EHYIRGYHELLTFSQTGIWNRNGRGAARDYILELIKNHYYRFEIGSDNRKPIFFRDFI
                     KNNREHEFNIYETLARDIIVHPLPFHLDYAPPKKGELVITQPENHYMQTVRHKLSHTA
                     PLLFDDLYGKESIFLTLELNSFKSNKKPNKRDFEHIFKCAQLVIHHLSKDGGDKILCS
                     TNGVKHHRLILSEIVAQCEHYQRHFNNKVVNHISKKIIKNNELSPHLKSVISSLDSNY
                     FNPWLMKLAVEKQLSWAEAHYKAQNHIENHIQDCELLGLMDVNKMLRDTITGFVHQIS
                     EITESSWMSSEEQHAKKIQALEMFKTKISSMNGGQEFVYGFNKVIQEGLGGLIELSFD
                     IDDTRHHRTLSSLSPASRSGLHLLGTIWNIVMSAVPGFNALAGTSSILNHAVVEKSTD
                     VCSYMQDVIRIGMEAIPAAESKFTQRSSNAKYTGLRFAEEKINKNIINEPQQRGSSYR
                     VIESIENTDFIYQKSTKKILELASIEGNELYSATGFNKENYGYYKRSGEGFYRKQHSY
                     QPLSNEMPNKIKYNNRELELTKESNSNIYSGTFTDNGKNSLVKFYKDSDGNFYQADGL
                     KGGGLIRHTDKPYSELKEGDIGYDEELLDITDDSPELEETLPAISEDLYPSEEENVQN
                     IYKKFKNGDHDAGMTEVTLCRGTIASQAENIVSYSTAGGAEIANPNVSPVSEDIAKLQ
                     IKSGRIEPEYTTDISVADRFSRGHHLVIVKAKVKYLTRGSISESGWIIPKNAPVEPVG
                     LIDRTFGQSENIQQANASK"
     gene            complement(2981..3403)
                     /locus_tag="ACHOSM_RS23845"
                     /old_locus_tag="pgaptmp_004769"
                     /pseudo
     CDS             complement(2981..3403)
                     /locus_tag="ACHOSM_RS23845"
                     /old_locus_tag="pgaptmp_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
     gene            complement(3406..>4097)
                     /locus_tag="ACHOSM_RS23850"
                     /old_locus_tag="pgaptmp_004770"
     CDS             complement(3406..>4097)
                     /locus_tag="ACHOSM_RS23850"
                     /old_locus_tag="pgaptmp_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HGU4561104.1"
                     /translation="ALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPTVPPSKRAH
                     TGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQD
                     VMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRAC
                     NGLSDNRCLEI"
BASE COUNT         1414 a          811 c          762 g         1110 t
ORIGIN      
        1 ggaattaaat ccactggcaa tggcccatcc tttaaagtca tttcagtatg catctgtagg
       61 gacggagcac aaactatttg atagcgaaaa attaaatata cagccggata atattgaaca
      121 agtaatatca aagggaaaag ctcttcagaa agattttttt gatagttata tagaaaataa
      181 agaaacctta cctattatca cgcaatcaga tggagttcac tataaaaaaa tcgcacttaa
      241 tggactatta cttgcctcag gaatatttaa accattaaac aacaactaca aaccacagag
      301 cggtcatgga aatttatttg atgaaattca tatcaggcac tcaaggtctc ttccagaaga
      361 acatgcggca ccagctgaaa aattaggtgt tcttattgca aaaaaacttg aagaatcaat
      421 agtaccaaaa ttcataccat tttacaatat tttaacaaaa ctagaacaag aacaagagca
      481 agaacattac attaggggat atcatgaatt acttactttt tctcaaacag gaatatggaa
      541 tcgcaatggc agaggtgcgg caagagatta catacttgaa ttaataaaga atcattatta
      601 cagatttgaa ataggaagtg ataatagaaa acccattttt tttagagatt tcataaaaaa
      661 taaccgagag catgagttca atatttatga aacacttgcg cgagatataa ttgtacaccc
      721 cctgccattt catttagact atgcccctcc caaaaaaggc gagctagtta taacacaacc
      781 agaaaatcat tatatgcaaa ctgtgaggca taagctttca cacactgcac cactactttt
      841 tgatgatcta tacggtaaag aatccatatt tttaacacta gaattaaata gttttaaatc
      901 aaataaaaag ccaaacaaaa gagattttga acatatattt aaatgtgcgc aattagtaat
      961 ccatcactta tcaaaagatg gcggagacaa aatactgtgc tccactaatg gagttaaaca
     1021 tcatcgcctc atattaagtg agattgtagc gcagtgcgaa cattaccaga ggcactttaa
     1081 taataaagta gtaaaccaca tatcaaaaaa aataataaaa aacaatgaat tgtcgccaca
     1141 cttaaaatca gtgatatcaa gtttagatag caattatttc aatccatggc taatgaaatt
     1201 agctgtagaa aaacagttat catgggcaga agcacactat aaagcacaaa atcatattga
     1261 gaatcatatt caggattgtg aattactggg cctaatggat gtaaacaaaa tgcttagaga
     1321 tactattaca ggttttgtcc atcagattag tgaaataacg gaatctagtt ggatgtcatc
     1381 agaagaacaa catgcaaaaa aaattcaggc tctcgagatg ttcaaaacaa agatatcatc
     1441 tatgaatgga ggtcaagagt ttgtttatgg ttttaataaa gttatccaag aagggcttgg
     1501 gggactaatc gagttaagtt ttgatattga cgataccaga catcatcgta cattatcttc
     1561 gcttagtccc gcatcaagaa gtggccttca tctattaggc actatctgga atatagttat
     1621 gagtgcagta ccaggattta atgcattagc aggaaccagc agtatactta atcatgcagt
     1681 tgttgaaaaa tctacggatg tatgtagcta tatgcaggat gtaatacgta ttggcatgga
     1741 agcaattcca gcagccgaat ctaaattcac acaaagatcc tcaaatgcca aatacacagg
     1801 ccttcgtttt gccgaggaaa aaataaataa aaatataatt aatgaacctc agcaacgtgg
     1861 ctcatcttat agagtgatag aatcgataga aaacactgat tttatttatc aaaaaagcac
     1921 aaaaaaaata ttggaattag caagtataga aggtaatgaa ctttatagcg ccactggatt
     1981 taataaagaa aactatggat actacaaacg ttctggtgag ggtttttata gaaaacaaca
     2041 ctcataccaa cctttatcca atgagatgcc aaacaaaata aaatacaata atagagagtt
     2101 agaattaaca aaagaatcaa actctaacat ttactcaggt acatttaccg ataacggtaa
     2161 aaattcatta gtaaagtttt ataaagactc tgatggtaac ttttatcaag ctgacggatt
     2221 aaaaggaggt ggacttattc gacatacgga taagccatat tcagaactga aagaaggcga
     2281 tataggatat gatgaggagc ttctcgatat tactgatgac tcacctgagt tagaggaaac
     2341 gttgccagcg atatcagagg acttatatcc tagcgaagaa gaaaatgtac aaaatatcta
     2401 caaaaaattc aaaaacggtg atcatgatgc aggaatgaca gaggtaacat tatgccgagg
     2461 aactattgcc tctcaggctg aaaacattgt ttcttatagt accgcagggg gagccgagat
     2521 tgcaaaccca aatgtaagtc ctgtttctga agatattgca aaactgcaaa taaaaagtgg
     2581 tagaattgaa ccagaataca caacagatat tagcgttgct gaccgattta gccgtggaca
     2641 tcacttagtt atcgtcaaag caaaagttaa atatcttaca agaggcagta tcagtgaaag
     2701 tggttggatc attcccaaaa atgcccccgt agaaccagta ggattaattg acaggacatt
     2761 tggccaatca gaaaacatac agcaagcgaa tgcatcaaaa taatatacat gcattagccg
     2821 tattgtctta gattggttca aacacagagt aacgtcaagc acctttcacg tataatctga
     2881 gtgtatcagc cacagacaac atctgatatt ttaaatgtct gtggcgtttt gatatagatt
     2941 actgatgtaa aatattactt tatagaaaaa tatattaact ttattttata acaaagtttc
     3001 ctttggtttg atcataaata cattgttctt gacttacaat cattgatgac gttattggtt
     3061 ctcgtgttag cgggtgcttc ccaccgtcac gaaccaaatg agaaaaagaa atcgaatcaa
     3121 ataaggtaca aaccaaagaa ccttctgaat ttttaacaaa cacaccttct tctggtttat
     3181 ccagtataat tgggcatcga accgcctcgg atgggcactg aaggctctgc gtattaaccg
     3241 aaaacttaca ttgtgatatt ttcccctcaa tatcaccatg tgattgtgtg ttacctgaac
     3301 ctgaaaccat tcttaacatt atatccctga gcgcttcagg accaccatta agccctgtaa
     3361 gtaataatcc agaaagcaaa ctgttattgc ctcctggatt tgcccctata tttccagaca
     3421 tctgttatca cttaacccat tacaagcccg ctgccgcaga tattcccgtg gcgagcgata
     3481 acccagcgca ctatgcggat gccattcgtt ataatgctcg aacgcctctg caaggttctt
     3541 tgctgccgtt aacccgtctg gtttgggcat gatactgatg tagtcacgct ttatcgtttt
     3601 cacgaagctc tctgctattc cgttactctc cggactccgc accgccgtgt tcttcggttc
     3661 aagtcccaac atccgggcga actggcgtgt ttcattagcc cggtagcatg aaccattatc
     3721 cgtcagccac tccactggag acgacggaag atcgttgccg aagcggcgtt ccaccgctcc
     3781 cagcatgacg tcctgtactg tttcactgtt gaagccgccg gtagtcaccg cccagtgcag
     3841 tgcctcacga tcacagcagt ccagcgcgaa cgtgacacgc agtctctctc cgttatcaca
     3901 gcagaactcg aacccgtcag agcaccatcg ctgattgctt tctttcacgg ccactctgcc
     3961 tgtatgtgcc cgtttcgatg gcggtacagt aggttttcgc tcaagcaaca gcgcattctg
     4021 gcgcatgatc cggtaaacac gtttggcatt gatcgcaggc ataccatcaa gttctgcctg
     4081 tctgcgaagc agcgccc
//
LOCUS       DAZKHC020000212         4085 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZKHC020000212 DAZKHC020000000
VERSION     DAZKHC020000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.213"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..1666)
                     /locus_tag="ACHOSM_RS23855"
                     /old_locus_tag="pgaptmp_004771"
                     /pseudo
     CDS             complement(1..1666)
                     /locus_tag="ACHOSM_RS23855"
                     /old_locus_tag="pgaptmp_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1612..1833)
                     /locus_tag="ACHOSM_RS23860"
                     /old_locus_tag="pgaptmp_004772"
     CDS             complement(1612..1833)
                     /locus_tag="ACHOSM_RS23860"
                     /old_locus_tag="pgaptmp_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561105.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVT"
     gene            complement(1878..3815)
                     /locus_tag="ACHOSM_RS23865"
                     /old_locus_tag="pgaptmp_004773"
     CDS             complement(1878..3815)
                     /locus_tag="ACHOSM_RS23865"
                     /old_locus_tag="pgaptmp_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU4561106.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRVREGLEITAMLVKPEPGMPSQMAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTYLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKYFDRPFS
                     AASGNRKPVVKIASSAGASAQSITSFHKEKSAMNTGEQIKSFENKRAALAASLEEIMN
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELETSKAATAQPVKQAGNGNVAT
                     VASAPVIRVEQKLEKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKARPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVTANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTSDSSTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGTASGG"
     gene            complement(3879..>4085)
                     /locus_tag="ACHOSM_RS23870"
                     /old_locus_tag="pgaptmp_004774"
     CDS             complement(3879..>4085)
                     /locus_tag="ACHOSM_RS23870"
                     /old_locus_tag="pgaptmp_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4561107.1"
                     /translation="GVLIHGDQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPLMA
                     LFNAVSLMSLNPEPKKKAYEVFFI"
BASE COUNT          839 a         1317 c         1032 g          897 t
ORIGIN      
        1 gccggtaccg gaatattcgc aaccgcatca ttcaccgcct gctgcagtac atccggatca
       61 taatcacgac catcacgcgg taccggaatg gtccccacag cgtcatccac catcgcctgc
      121 agaaccggat gtacctcatc caccgtcaca tgcttctgta ataccgccga cagggaagcc
      181 agtttctctt caaacgcttg tgcctgcgcg gccatcttcc cctcaaatgt gcgctgtaaa
      241 tccgccagca ccgtggagaa ttcttcgccc agtgcacgaa taatggacag ttcccgttcc
      301 gtcattttct cagtatcccc ctgaacatcg ctttcactgc atcatgctct gtttcactga
      361 ttgccttatt accgtcagat gcgccgtcag gcagttgtgc tgaaactgtt ttcccggccg
      421 acgcgaacgg gtcctcacgg gcatcacgac gggacagcgc ctccagactg tagttctgct
      481 gctgaagata cagtgcatca ccgccggcaa ggggcggcag gttctcacgt ttacgggcct
      541 cattgggcgt gagaagcgta tttttcaccg attcacccag tgttttcatg cgccgttcgc
      601 tgtccattct cagcagcgtg gtgacgtcaa actccgtgct ctcgttttcc cccgtttcca
      661 gcgcctcatc cagtaacagc tcaatggact caatcagcgt ctgcagacac tgggaataat
      721 actgctgctc cagcgcctcc acgttgtcac tggaaggcgg gtggccaacg ccaatcttgt
      781 aggccgggac acggaacacc gaacagacaa tttcagccgt cattttcagt tgttccaccg
      841 tctgcgcatc caccggtgaa aacgtcgtgg ggctgtattt tgccccgttg ctcagtatgg
      901 ccgtcttccc ggcattttcg cccgtatatc cgctgtccca gttccccttc agttttttcg
      961 cgttttcttc cgtaatactg ccggggacct caatcacgcc tgatggtcgc ccgccatttc
     1021 tgaaaaaata cgtcgaattt gcctgaatat gatgcccctg catggccgcc agcccggcgg
     1081 catacaccgg cggcagcccc acaagcggat gaaaaaaaca gttaaaccgg tcgtggatca
     1141 cttccctggc aggcaccgtc accgcctccg tgatcccgca gttccggtcc ggcgtgatgc
     1201 gatagaacac ctcgccgtca tccgccacca gaggttcaac ccggttccag tccagaatac
     1261 gcagttcttt gatctgcccc cgggagttac ggattttcag caccaccgta ttgccgtgac
     1321 gcagtttggc gttcagccac agttcaaaaa actggatacg attctgctgt gcattaggac
     1381 gacgacagag acgggcaata tccccctgcc gtttttcacg gcggatcccc tgtgtatcgg
     1441 tctgcatcag gcgcagtcgc attttggcga tatcctggga tatcagcgaa atgcaagaaa
     1501 acaccgcgtg aaaggagagg acactttccg gatcggcttt cacgccctgc tgccaggcgc
     1561 cggcaaaagg ctcagccacc gcctgaaaca gggaccgcca gcccacttct cttacgtcac
     1621 gtcctgattt ctggtttttt cgggttcgcc gcaaaaggtt ccacattcgc catgctccgc
     1681 atcacgtttc tttttctgac ctgccggacg tcgcgctgtg atgtactccg ccttccccag
     1741 gcgaaccagc acctccgcac acggctgtgc cacatcacgg atatccccgg cccgggcatc
     1801 atgcgtgccc tgcagatact ggatttttgc catctgttac tgcgggaagc tcgcgcctcc
     1861 cgccctcctc atcagactca gccaccggac gcagttccgt agttcacacc ggtgatcacc
     1921 gccactgccg cggtacggcg acgacgccag ttgatccagc gctccgcacg gatggccacg
     1981 ctgccggtct gaaacatgga gaccagttcc accggtgacg gtgtgctgct gtcgctggtc
     2041 ggttcagact gcatctccag tgacgcttca cgggacatat ccaccgccac accgccgtca
     2101 tcagccagat aaatatccgg tgcattcacc agtaccagct ggtcacccac atactgggag
     2161 acaattaccg gaagcccctg gaaggtcccg cccagcaagg tcatgtccgg atattccttc
     2221 tgccccagcg catttttacg catggacagc gccagggcat tcgtgctgga catcagccag
     2281 accgcaccgg tgggctgcag atttgccgtc acaaactggc caaacgcggc ctcggcatcc
     2341 gcatccgggt taccggttga tgccgtgccc ttcacatcat gggtgatgga cgccggggag
     2401 acatccgcca ccgccgcttt tttcgggtcc acaaagtctg tgtccagacg cgccaccacc
     2461 gcttccgcca gcgcattacg gaccagtgca tcagcagccg gactggaaaa acggatcaat
     2521 tcttccgtca gtaccgcaat ggccgacact ttcgcatgac tgaaggtgat ggattcaaaa
     2581 tcaaacttcg tcaggggtct ggccttaccc tcacccaccc agccggcagc accaccggac
     2641 acctgggcat gcacgcggat attgaacggc acctgacgaa gtgcagggat cccgccctga
     2701 ccaaatcgcc cgataatggt ctgcggacgc aggtaatcaa taaagtcctg cgcatattcc
     2761 tggtattcag acaggctgcc tgcccactgc gggtccgtgg tggtccctgc ccccaccgcc
     2821 gatttcagga catgatgcag acgactgtca tccggatact gacgacgggc cacttccagg
     2881 gcttcagagc ggacaccttt agccgcggcc agtgatttgg caaagcgggc gaaaccaatc
     2941 cccttctcca gtttctgctc aacacggatc accggcgctg aagccaccgt ggccacattc
     3001 ccgttaccgg cctgtttcac cggctgtgcc gtggcggcct tactggtttc cagttcacgc
     3061 agacgcttca ggtgcgcatc cacctgacgg atttccgctg cggtgttgtc gtagtgctct
     3121 tcctcttcca catccagtgt gcggccttcc tctgcggctt tgttcatgat ctcctcaagg
     3181 gaggctgcca gcgccgcacg cttgttttca aaacttttaa tctgttcacc agtattcatt
     3241 gctgactttt ccttatgaaa agaggttatt gactgtgccg aagcgccggc agaagatgcg
     3301 attttcacca ccggtttccg gttgccggac gcggcagaaa acgggcggtc gaaatattta
     3361 atggtccgga tggtgcattc cgcattcgcg ggcacggtga cggcagacac ctccatcagc
     3421 tcccagcgca gaaaatgcag tccgcctccg tccagataag tgtattcatg gggccggaag
     3481 cccacagaaa gccccctgac cagcccggtc ttaatggcag cccaggcctc atccagccgg
     3541 gctgccatct gggagggcat ccccggctcc ggcttcacca gcattgccgt gatttccagc
     3601 ccttccctga cccgacgcac cgtacactgg cctacagggc gggaatggtc atgctgccag
     3661 agaaacggga tcgtactgcc aaactccgcc ccctccggct ccaggatgtc accatcccga
     3721 tccggagaag gcgttgacgc aatcccggtg atcacccgtt catcctcact gaaggatttc
     3781 accgtcagca gggaacaggc ccgtttaaga gtcacatcag cctcctgaaa ataaaaaaac
     3841 cgccgcagcg gttcatgatg gttacagggt gagcagggtt atatgaaaaa aacctcatac
     3901 gctttctttt tcggttccgg attcagggac atcagggaca ccgcattgaa gagcgccatc
     3961 agcgggtcaa tttttccccg tccgctggcc tgtttggtga taagaatggc gttaccttta
     4021 ggctccaccc gggcattacc gacacaccag gccatcaggg gctgatcacc gtgaatcagc
     4081 acccc
//
LOCUS       DAZKHC020000213         4081 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZKHC020000213 DAZKHC020000000
VERSION     DAZKHC020000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.214"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            130..438
                     /locus_tag="ACHOSM_RS23875"
                     /old_locus_tag="pgaptmp_004775"
                     /pseudo
     CDS             130..438
                     /locus_tag="ACHOSM_RS23875"
                     /old_locus_tag="pgaptmp_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            821..874
                     /locus_tag="ACHOSM_RS23880"
                     /old_locus_tag="pgaptmp_004776"
     CDS             821..874
                     /locus_tag="ACHOSM_RS23880"
                     /old_locus_tag="pgaptmp_004776"
                     /inference="COORDINATES: protein motif:HMM:NF023742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4561108.1"
                     /translation="MLRTTKVHIYPTPEQAE"
     gene            956..1338
                     /locus_tag="ACHOSM_RS23885"
                     /old_locus_tag="pgaptmp_004777"
                     /pseudo
     CDS             956..1338
                     /locus_tag="ACHOSM_RS23885"
                     /old_locus_tag="pgaptmp_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942659.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            complement(1363..2392)
                     /locus_tag="ACHOSM_RS23890"
                     /old_locus_tag="pgaptmp_004778"
                     /pseudo
     CDS             complement(1363..2392)
                     /locus_tag="ACHOSM_RS23890"
                     /old_locus_tag="pgaptmp_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            2494..2712
                     /locus_tag="ACHOSM_RS23895"
                     /old_locus_tag="pgaptmp_004779"
                     /pseudo
     CDS             2494..2712
                     /locus_tag="ACHOSM_RS23895"
                     /old_locus_tag="pgaptmp_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            3014..>4081
                     /locus_tag="ACHOSM_RS23900"
                     /old_locus_tag="pgaptmp_004780"
     CDS             3014..>4081
                     /locus_tag="ACHOSM_RS23900"
                     /old_locus_tag="pgaptmp_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HGU4561109.1"
                     /translation="MNVDTLNTNNVIKQTRQRTSERKNLYFLEKNRKIRHLSVDDVIS
                     IGGIIQSEIRRTLHPSPLPATTSARNWKSVIIHYIGLLISKPLQMPFFSYNLQQDPKD
                     VTSLPHHIPENNKEKLIPTGIKEHPEYVRENNIQTQVIHKREKRHTNNIKKTEELIQA
                     VESNTKYSLIANNIKDKIFGHVDTVSPKDKKLIKILKDEIKNYIFLPEKNSRKGIESL
                     KNQASILEKLRAEASPAVQDNISEIITSLHNEYKSLKVEIDKKIHVIWIAGAPPETIT
                     KYAKAYKAAYPNFSFNLWIDPNAFAAYEFNSQLKKAALEHAKSEVIDSLTVEELNVLK
                     NKEQLDDGFHAKLKTLFETNLL"
BASE COUNT         1251 a          873 c          901 g         1056 t
ORIGIN      
        1 gtatcctgaa atagcccgct gcacggagcg ggagtttgcg accattcttg ccggtatcag
       61 cgccggcggc atgtactgcc cggagtgtgg cacggttcac tggccggacg gagtcccccc
      121 gcccttctga tgcttccccg ttttgccgac atttttcagc agggaaaccg ctggcttaac
      181 tggctggaga aacaaccgga aggttcagtg cgtccggtag tcattgagtc tgtgacaaaa
      241 atcatggcct gcgggaccac gctgatgggg tacacacagt ggtgctgttc atctccggac
      301 tgcagccaca taaaaaaggt ctgcttccgg tgtaaaagtc gctcctgccc gcactgcgga
      361 gtgaaggctg gcgcacagtg gatacagtat ctgctgagtc tggttcccga ctgtccgtgg
      421 cagcatattg tgttctccaa tgactagtct aaaaactagt attaagacta tcacttattt
      481 aagtgatact ggttgtctgg agattcaggg ggcaagaaca aatacctaat tgacagtatc
      541 atcatgctga ttgttaggat ttagaaaata tgactaattt tacatgattt tgcatctttg
      601 caaatcatgt gcggtagctt tgctgttttt ataattaccc gtgttagttc gaaacaccag
      661 ataatcaatc gaggtagttt ccttatatga aatcagtcag caaaaaaccg acgagccccc
      721 tcgcccaatt cgggcgggga ggaaaagtgg aaaagcccga agggcttaaa aaaagcattg
      781 cggtattagc ataaaaagcc tgtatcaatg agcagtgact atgttaagaa cgacaaaagt
      841 acatatctac ccgacaccgg aacaggcaga gtaagtatct caatgctcgg ttcgagcgca
      901 gtacattttg cgtacaataa agatcttcac atcaaaaaac acgcttacca gcaatatggg
      961 gcttgagcac tatgccatca tgtctaatga ggaaaaaagt tccgaatcct cgtcatctca
     1021 ttaatgcctg ccgcaatctg cgacgaaaac aaaaagcgtt atcccgcaag cgcgaaccgt
     1081 aaaaaggcca gaatccgtct ggcagactta cacgaacggg tagcgaatgc tcgtgctgat
     1141 tttcagcata aactttctcg tacaattgtt gacgaaaacc aagcggtaat tgttgagacg
     1201 ctgaaaacag ccaacatgat gaaaaacaac aatctggcaa gagcgatagg tgatgcaggc
     1261 tggcatagcg tcatcacaaa gctggagtac aaagcagcgg gaaaaggcat ttacctagta
     1321 aaactcgacc aatggtttga taaataactg atcctaatac agctacactt tagcctgccc
     1381 atggtttcct ccggggaatg gtctgacgtt ttccataaaa tggcgaacct ttttcagcag
     1441 ttgccacatt gaatagcact ggtgatttcg ggttattgtc tcatgaagtg tctgccacaa
     1501 ccgttcggca tgattgacct acggcgagta aaccggctga taaatgcccg tgaactttgg
     1561 gtttgctttc agtcagcgca acgtttcacg gcttttgtgg atgatgtaat tatcaacaat
     1621 gagcgtaatc gttttttccc accggtatgt cgctttcaga tttttaacag aatgataaat
     1681 aaggacgaac ctttactatt tccaccaacg tagctcactt ttcccgtatt gctgtgcagc
     1741 gcaccggcaa gataggacgt ttcattctgg cccggcgtca caacgcgctt ttgctgccca
     1801 cgtaactgcc agtctgcgcc aattttggga ttgaggtgaa tatccacttc atcatcgtaa
     1861 aacacggggt tttccgcact gcatttcgcc aatgcttcat ggatagcgac cattttttct
     1921 tctttgtgtg ggtcacggat acacagtgtt ggcacggttc tctaccatac caatccggca
     1981 gctggcaacc attgccggat ggttgaagaa tgtagtggac aatcggtgac atcacggatt
     2041 tttatcgcca gcagttcggt actccagctg gaacgctgat aaccgaaatc gccgggagaa
     2101 tgcttaacaa gctcatgcag caatgcgcaa atatgttcga atagccaacg tcttccacgt
     2161 cctgatggta atgacttcag gccttcaaca ccagacagcg tgaaccagtt aatccagcga
     2221 ccaatggatg agcgggtact gcagagcgtt ctggcaacat gactgacggt gtcaccacga
     2281 tgcagcatca gcatggggtg agccgccggg cgtggttttt gtgctttgtt ttatagatag
     2341 ttttctgcat cagacgtcgt tcggttctgg gtattagtgc tatgatcgcc attacgcagt
     2401 ccggtgggtg atttgggata ttcagcgatt gatcagatcg cataaaccgg actgagttcc
     2461 cccggagtga tctactattc cgagcagtta tttagttcga aaaggtgcca ttgttgtggt
     2521 tacaaaatgc cggaaatgcc gtttcataaa tgcatctggc gatgccctga atgtcgagca
     2581 gagcacgacc gcgatatcaa tgcggcactc aacatccggc aaaaaggaat actggaacta
     2641 aaggcggcgg gactcgtcgc ctctgcctat agagggcagc atagatccgt cgtacagaca
     2701 gttgcggcct gagaagtggg aagcctcgcc cgacagggcg gggagcagta acataaagac
     2761 tcatccttac tctgctcttt cttcctgggc agaagcatca tgcgctgacg cttatggttc
     2821 gggaaatatc cagaaataca gatagtcgcg agtgttcgta gcgatttcat cacacaccga
     2881 aaaaaaagca cctcgttttt ctatgcaagg aagtggatta ctggtcgatg attcacttat
     2941 atttccccgc ggtcagaaaa cttaccgata ttcacataaa aagtataggt gataaattat
     3001 ataggaggtt actttgaatg tagataccct aaatacaaat aatgtcataa aacaaactcg
     3061 ccaaagaaca agtgaaagaa aaaacctcta ttttcttgaa aagaacagaa aaataagaca
     3121 cttgtctgta gatgacgtaa tttctatagg tggaattatt cagagtgaaa taagaagaac
     3181 tctgcatcct tcacctttac ctgcgaccac atctgccagg aactggaagt cggttattat
     3241 tcattatata ggtttgttaa tatctaaacc tttgcagatg ccattttttt cttataattt
     3301 acagcaggac cccaaagatg taacttcgtt acctcatcat ataccagaaa ataacaaaga
     3361 gaaactaata cctactggta tcaaagaaca tccggaatat gtaagagaaa ataacatcca
     3421 aacgcaagtg attcataaaa gggagaaaag acatacaaac aacataaaaa aaacagagga
     3481 gcttattcaa gccgtggaaa gtaacacaaa atactcacta atagcgaata atataaaaga
     3541 taagatattt ggtcatgttg atacggtgtc ccccaaagat aagaaattaa taaaaatcct
     3601 taaggatgaa attaagaact atatttttct tccagaaaaa aatagcagaa aaggcattga
     3661 aagtctaaaa aaccaagcaa gtattttgga aaaacttcgg gctgaagcat ctccagcagt
     3721 tcaagataat atatctgaaa taattacttc attgcataac gaatataaat cacttaaggt
     3781 ggaaatagat aaaaaaattc atgtaatttg gatcgctggc gcaccacctg agactataac
     3841 aaaatatgca aaagcatata aagcagctta tcctaacttt tcttttaatt tgtggataga
     3901 tcctaacgca tttgcagctt atgaatttaa ctctcaatta aaaaaagcag cacttgaaca
     3961 tgcaaaaagt gaagttattg acagtttaac tgtcgaggaa cttaatgtat taaaaaataa
     4021 ggaacaactt gatgatggtt ttcatgccaa attaaaaacg ctttttgaaa ctaacttatt
     4081 a
//
LOCUS       DAZKHC020000214         4004 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZKHC020000214 DAZKHC020000000
VERSION     DAZKHC020000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.215"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..2832
                     /locus_tag="ACHOSM_RS23905"
                     /old_locus_tag="pgaptmp_004781"
     CDS             <1..2832
                     /locus_tag="ACHOSM_RS23905"
                     /old_locus_tag="pgaptmp_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HGU4561110.1"
                     /translation="SQPGTLNDFLGAMTEDDARPEALRRFELMVEEVARNASAVAQNT
                     AAAKKSAGDASTSASEAATHATDAAGSARAASTSAGQAASSAQSASSSAGTASTKATE
                     AEKSAAAAESSKSAAATSAGAAKTSETNAAASQQSAATSASTATTKASEAATSARDAA
                     ASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSETNAKSSETAAEQSASAAADS
                     KTAAASSASAASTSAGQASASATAAGKSAESAASSASTATTKAGEATEQASAAASSAS
                     AAKTSETNAKASETSAESSKTAAASSASSAASSASSASASKDEATRQASAAKSSATTA
                     STKATEAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLS
                     SATNSTSESQAATPKAVKAAYDLANGKYTAQDATTAQKGIIQLSSATNSTSETLAATP
                     KAVKTAYDNAEKRLQKDQNGADIPGKDTFTKNIGACRAFGGSVSTITGNWTTAQFIEW
                     LDSQGAFNHPYWMCKGSWSYGNNKIITDTYCGNIHLAGAVIEVMGIKSAMTIRITTPT
                     TSTGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPTASEIGALPSGGTAVSSVNLASK
                     GRVTALTDNTQGATGLELYEVYNNGYPTAYGNIIHLKGMTAVGEGELLIGWSGTSGAH
                     APAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVGAPIPWPSDTVPSGYALMQGQTF
                     DKSAYPKLAAAYPSGVIPDMRGWMIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLG
                     TKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFNPSVFKDTYQYGYTSS
                     GQNTWGVQGSVGMSTGWLANTSTDGNHSHSLSGTAASAGAHAHTVGIGAHTHSVAIGS
                     HGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            2832..3413
                     /locus_tag="ACHOSM_RS23910"
                     /old_locus_tag="pgaptmp_004782"
     CDS             2832..3413
                     /locus_tag="ACHOSM_RS23910"
                     /old_locus_tag="pgaptmp_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4561111.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     NSLLEAWKKYRVLLNRVDTSTAPDIEWPVIPEV"
     gene            complement(3533..>4004)
                     /locus_tag="ACHOSM_RS23915"
                     /old_locus_tag="pgaptmp_004783"
     CDS             complement(3533..>4004)
                     /locus_tag="ACHOSM_RS23915"
                     /old_locus_tag="pgaptmp_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HGU4561112.1"
                     /translation="TADLRSNIAAEARAKIIYERLINVTDDPGVKDTLAFLMTREAAH
                     MLSFEKALHSIRNTFPPGKLPPIEKYKNVYYNMSEGEDVRGSWNSDENFDYVSDPVPA
                     VDGGDGKASINLSTKQEAMIKAMATRLKSHEDINPVTGAELAEGEPQTKINSKN"
BASE COUNT         1096 a         1009 c         1059 g          840 t
ORIGIN      
        1 tcacaaccgg gtacgctgaa tgattttctt ggtgccatga cggaggatga tgcccgtccg
       61 gaggcactgc gccgttttga gctgatggtg gaagaggtgg cgcgtaacgc gtccgcggtg
      121 gcacaaaaca cggcagccgc gaagaagtca gccggcgatg ccagcacatc tgccagtgag
      181 gcggcaaccc atgcgactga tgctgcaggc tcagcacgcg cagccagcac gtcagccgga
      241 caggccgcgt cgtcggctca gtcagcgtct tccagcgcag gaacggcatc aacaaaggcc
      301 actgaagcgg aaaaaagtgc tgccgctgca gagtcctcaa aaagcgcggc ggccaccagt
      361 gccggtgcgg cgaaaacgtc agaaacgaat gccgcagcgt cacaacaatc agcagccact
      421 tctgcatcca ccgcgaccac gaaagcgtca gaagctgcca cctcagccag ggatgcggcg
      481 gcctcaaaag aggcagcgaa atcatcagaa acgaacgcat cctcgagcgc cagtagcgca
      541 gcttcctcgg caacggcggc aggaaattcc gcgaaggcgg caaaaacgtc cgagacgaac
      601 gctaagtctt ctgagacggc agcggaacag agtgcctccg cagcagcaga ctcaaaaaca
      661 gcggctgcat catctgccag cgccgcgtca acaagtgccg ggcaggcctc agccagtgcc
      721 accgctgccg gaaagtcggc agaaagtgcc gcatcgtctg cttcaacagc cacaacgaag
      781 gctggcgaag ccactgaaca ggccagcgca gcagcgagtt ctgcttccgc agcgaagaca
      841 tccgaaacga acgcgaaagc gtcggaaacc agcgcagaat cctcaaaaac ggctgccgca
      901 tcgtcagcca gttcggcggc gtcatcggca tcatctgcgt ctgcttcaaa agatgaggcg
      961 accagacaag cgtcagcagc gaagagcagc gccacgacgg catccacgaa ggcgacagag
     1021 gcagctggca gtgcgacggc ggcagctcag agcaaaagta cggcggaatc cgcggcaacg
     1081 cgcgccgaga cagcagctaa acgggcagag gatattgcat ccgccgtggc gcttgaggat
     1141 gcgagcacga cgaaaaaggg gatagtacag ctcagcagtg cgaccaacag cacttccgag
     1201 tcacaggcgg caacgccaaa agccgttaag gccgcgtatg acctggctaa cgggaaatac
     1261 accgcacagg atgcaacgac agcacagaaa gggataatcc agctaagcag cgcgaccaac
     1321 agcacatctg aaacgcttgc cgcgacacca aaggcagtaa aaacagccta tgacaatgct
     1381 gagaaacgtc tgcagaaaga tcagaacggt gcggatatcc ctggcaaaga cacctttacg
     1441 aaaaatattg gtgcctgccg tgccttcggt gggtcagtaa gcacaataac aggaaactgg
     1501 acgactgcac agtttatcga gtggctggat tctcagggag catttaacca tccctactgg
     1561 atgtgcaagg gttcctggtc ttatggcaat aataaaatca ttactgatac ttactgcggt
     1621 aatattcatc tcgccggagc tgtcattgaa gtaatgggga taaagtcagc gatgacgatc
     1681 cgcattacca caccgaccac ctccactggt ggtggaacaa ctaacgccca gtttacctat
     1741 attaatcacg gaacagatta ttcacctggc tggcgaaggg actataactc cagaaataag
     1801 ccaacggcat cagagatcgg ggcgttaccg tcaggtggaa cagcagtatc atcagttaat
     1861 ctggcttcaa aaggtcgggt aaccgcgctg acagacaata cgcagggggc aacaggtctt
     1921 gagttatacg aggtgtataa caacggatat ccaacagcgt atggaaatat cattcacctg
     1981 aaagggatga cagccgttgg cgaaggtgag ttactcatcg gctggagtgg tacaagcggt
     2041 gctcatgctc cggcatttat tcgttcacga cgggatacga ccgacgcaaa ctggtcgccg
     2101 tgggcgcagc tttacacctc ggctcatcct cctgcagagt tttatccagt cggtgcacca
     2161 atcccgtggc catcagatac cgttccgtct ggttatgccc tgatgcaggg gcagactttt
     2221 gacaaatctg cctacccgaa acttgcagcg gcttatccgt caggcgtgat ccctgatatg
     2281 cgtggctgga tgattaaggg caaacctgcc agtggtcggg ccgtattgtc tcaggaacag
     2341 gacggcatta aatcgcatac ccacagcgcc agcgcatcca gtacggattt ggggacgaaa
     2401 accacatcgt cgtttgatta cggcactaaa tccacgaata acactggtgc gcatacccat
     2461 agtttaagtg gcagcacgaa tgcagctggt aatcacagcc atagagatgg ccgtcgattt
     2521 aaccccagtg tttttaaaga tacttatcaa tatggttata caagctcagg tcaaaatacc
     2581 tggggtgtac aaggctcagt aggtatgtct acggggtggt tagctaatac cagtacagat
     2641 ggtaatcata gccactcact gtccggcaca gcagcatctg caggtgcaca cgcgcatact
     2701 gtcggtattg gtgctcatac gcactccgtt gcgattggtt cacatggaca caccatcacc
     2761 gttaacgctg ctggtaacgc ggaaaacacc gtcaaaaaca tcgcatttaa ctatattgtg
     2821 aggcttgcat aatggcattc agaatgagtg aacaaccacg gaccataaaa atttataatc
     2881 tgctggccgg aactaatgaa tttattggtg aaggtgacgc atatattccg cctcatacag
     2941 gtctgccagc aaacagtacc gatattgcac caccagatat tcctgctggc tttgtggctg
     3001 ttttcaacag tgatgaggca tcgtggcatc tcgttgaaga ccatcggggt aaaacggttt
     3061 atgacgtagc gtcaggggac gcgttattta tttctgaact cggtccgtta ccggaaaatg
     3121 ttacctggtt atcgccggaa ggggagtttc agaagtggaa cggcacagcc tgggtgaaag
     3181 atgcagaagc agaaaaactg ttccggatcc gggaggcgga agaaacaaaa aacagcctga
     3241 tgcaggtagc cagtgagcat attgcgccac ttcaggatgc tgtagatctg gagatcgcaa
     3301 cggaggaaga aaactcgttg ctggaagcct ggaagaagta tcgggtgttg ctgaaccgtg
     3361 ttgatacatc aactgcacct gatattgagt ggcctgtaat accagaagtt taaaataaaa
     3421 gcccgcttaa aatatcgcgg gcttcgaaat ataaatgtat tttcagaggc tagagcttag
     3481 gatatctcag cggcaattaa cttatgaggg atagctaaat accagaagat aattagtttt
     3541 tactatttat ctttgtttgt ggttctcctt cagcaagctc agcgccagtg acaggattga
     3601 tgtcttcatg ggatttcaac cttgttgcca tagcttttat catagcttct tgcttggtcg
     3661 aaagatttat cgaggccttc ccatccccac cgtcaaccgc tggaactgga tcggaaacat
     3721 agtcaaagtt ttcatcactg ttccaactac ctctgacgtc ttcaccttct gacatgttgt
     3781 aatatacgtt tttgtatttc tcaattggtg gtaatttccc tggtggaaag gtgttacgaa
     3841 ttgaatgtaa tgctttttca aatgaaagca tgtgtgctgc ttccctggtc attaaaaatg
     3901 ccagagtgtc ttttactcca ggatcatcag taacattaat gagacgttcg taaatgatct
     3961 ttgcccgagc ttcagctgca atatttgaac gaagatcggc cgtg
//
LOCUS       DAZKHC020000215         3981 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZKHC020000215 DAZKHC020000000
VERSION     DAZKHC020000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.216"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            18..235
                     /locus_tag="ACHOSM_RS23920"
                     /old_locus_tag="pgaptmp_004784"
                     /pseudo
     CDS             18..235
                     /locus_tag="ACHOSM_RS23920"
                     /old_locus_tag="pgaptmp_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            219..365
                     /locus_tag="ACHOSM_RS23925"
                     /old_locus_tag="pgaptmp_004785"
     CDS             219..365
                     /locus_tag="ACHOSM_RS23925"
                     /old_locus_tag="pgaptmp_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HGU4561113.1"
                     /translation="MFPTLYGVIIGVMPQPRRMQDVLRLAGELSIVRVVNDFQPLPIL
                     LCFQ"
     gene            complement(447..1604)
                     /gene="intS"
                     /locus_tag="ACHOSM_RS23930"
                     /old_locus_tag="pgaptmp_004786"
     CDS             complement(447..1604)
                     /gene="intS"
                     /locus_tag="ACHOSM_RS23930"
                     /old_locus_tag="pgaptmp_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HGU4561114.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYANELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKEIRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NENEWPADAIEMQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKPE"
     gene            complement(1766..1840)
                     /locus_tag="ACHOSM_RS23935"
                     /old_locus_tag="pgaptmp_004787"
     tRNA            complement(1766..1840)
                     /locus_tag="ACHOSM_RS23935"
                     /old_locus_tag="pgaptmp_004787"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1805..1807),aa:Arg,seq:cct)
     gene            complement(1916..2848)
                     /gene="yfdC"
                     /locus_tag="ACHOSM_RS23940"
                     /old_locus_tag="pgaptmp_004788"
     CDS             complement(1916..2848)
                     /gene="yfdC"
                     /locus_tag="ACHOSM_RS23940"
                     /old_locus_tag="pgaptmp_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGU4561115.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3142..3897
                     /gene="mlaA"
                     /locus_tag="ACHOSM_RS23945"
                     /old_locus_tag="pgaptmp_004789"
     CDS             3142..3897
                     /gene="mlaA"
                     /locus_tag="ACHOSM_RS23945"
                     /old_locus_tag="pgaptmp_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HGU4561116.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          938 a          977 c          956 g         1110 t
ORIGIN      
        1 aatagctata tgcgtacatt ctcaacctgc ccgtcacgta gcaggttgaa tccctttttc
       61 ttgataaagg gctggtcctg ccaccgttgt tgttgggtgc gttaaaaata cactactaat
      121 atgcccggca tgcgggcaac agagtcctgt tcacgatcgc cgccaccgga aatggcgctg
      181 cctcgatacc tgccagttca tggcactatt cgaagccgat gttccccacg ttatatggag
      241 taatcattgg ggttatgccg cagccacgtc gtatgcagga cgtgctgcgg ctggctggcg
      301 aactttcgat agtgcgagta gtgaatgatt tccagccgtt accgatttta ctatgttttc
      361 agtagaacgc ttagacaaaa ctgaggcaca caaagctttg tactgggttg caaggctttg
      421 tgctattcga tagttgttaa ggtggatcac tcaggctttt catcaagcca gtccgcccac
      481 cactgcatca tttctctgcg cttatcgaga tactgcgcat ggttgtaaat accgcgcaca
      541 gatccgccgt tggcatgtgc cagttgcatt tcaatagcat cagcaggcca ttcgttctcg
      601 ttcataatcg tgctgaattc atgcctgaat ccgtgaccgc tttccagacc ctcatagccg
      661 atttgtttga tcacaagcag taccgcattc tcgcagattg gcttcttctt atcgttgcgc
      721 ccggcaaaaa caaattctga tactggttta gtgatggagc ttagcgtagt gagaagttca
      781 accacctggt ctgacatcgg gaccacatga attttgcgtc ccttcatcac actggcgtcg
      841 atggtgataa tcctgttttc aaaatcgacg ttcttccata gcatggagcg aatctctttc
      901 gttcttaggg ctgtgtagcg caaaacttta gtcgcaatga gcgatacgat acttcctgaa
      961 aatgttgcca gtgctttatt gaatgccggg atctggtctg caggaagaaa cgggaagttc
     1021 ttcttgcggt atcccttcat ggcgtcagca aggtcaggtg ccgggttata tttagcccta
     1081 ccagtgacaa tagcgtaacg gaaaacctcg ccgcatcttc tgcgggcttt atttgctcgc
     1141 tccattgcac cgcgatcttc aaatctgcgg attacttcca gcagttgcat cggctcaata
     1201 tcctgaattt caaggccgcc gatgataggt aaaatgtcgt catcaaacat ttttgcaagt
     1261 tcatttgcat agcctactga ccagacttgc ttcttgtgct cgtaccattc cttgtaaatc
     1321 gcactaaatg aattgttgtt agacgaagcc tttttcgcct ttaccggatc gatgccaacc
     1381 gagatgtctt tcctcgcagt ccatgcctta tcccttgcct cctgcaaagt cattagcgga
     1441 tattttccta cggtcaggat tttctcctta ccgtcaatct tgtagcgaag ctgccatacc
     1501 tttttcccgg atacagggac ataaaggtac aggccattac catcgagaag gcggtatggt
     1561 ttttctttcg gctttgctgc ttcaatctgc ttaacggtga gcatgggtaa aaatccggtg
     1621 ggtaaaatta ctttatccac tttttacccg tcatggagtg cggctgtcaa cgatctgacg
     1681 cgaaccatta cgaactgtga atctacggaa gacttgatat tcaggggatt ttgcggactg
     1741 gtacggatgg gggcgaactg ataaatggtg tcccctgcag gaatcgaacc tgcaattagc
     1801 ccttaggagg ggctcgttat atccatttaa ctaagaggac aatgcggcat gagtataccc
     1861 gctaatggac tgcggggtaa gtacgctgcc gctcgattgc ttaaaccctc gccatttatg
     1921 ctgggttttt atcatttttc ttaatgtttt ccgcacgttc tgctttttgg cgtgcttctg
     1981 ctttacgctt gttgctcatg tcgttacgaa tctgtgcatg actcattaac gcgaagataa
     2041 aggtgccgcc gcagatgttc cccgctaaag taggtagtgc gaagggccag atgaaatcgc
     2101 tccagtgcag cgtgccgtta aacaccagat agaggatttc aacagaaccg acaacgatgt
     2161 gggtggtgtc acccagtgca ataagccagg tcatcaatat aatcaccaca atctttgccg
     2221 cacccgcagc aggaaacatc caaaccatag tggcgatcag ccagccggaa atgatcgcgt
     2281 tggcaaacat ctcgctgggg gtgttcttca tcacatccat gccgattttg acaaatgcat
     2341 cgcgagtttc ttcattgaag ataggcatat attcaaatgc ccatgcagca atacctgtcc
     2401 cgagaatatt acccagcagc acgacgcccc ataatcgcat aagtaagccg acgttgctca
     2461 ttgtcggttt ttgcatgacg ggtagtaccg cagtcacggt gttttcggta aataattgct
     2521 ggcgggccat aatgacgata ataaaaccaa aggtataacc gagattctcc agtaagaagc
     2581 tgcctggcac tccttccagt tcgacatgaa atatcccttt tgccagtagc gaagcgccca
     2641 tcgacagacc cgccgcaatg gctgaccaca gtagcgccat tgcgtcgcgt tccagctctt
     2701 tttcaccatc ctggcggata tgctcatgaa ttgccatcgc ccgggagggg agtcggtctt
     2761 catctatttc tatttttttg ccgcgctctt tttcttcgct ctcaacttca atttcgtcgc
     2821 tgtgttgatc aattttgtcg ttgtccatgg tctcttctct tgaattagca cgtatagcta
     2881 aagcgtagcg gcttttttgc tcgcaactgg cgggagttac tctgaaaatg tagaaaaggc
     2941 tgcgtttgcc tttttctgtt tctatagaat caagtagcct acagggcggc gattaccagg
     3001 ctatgatcaa atcagcaaat cagggcgtct ggacatcagt tgacgtgctg ttacaatcgc
     3061 ccacacctaa acaggcggat acggtatcgt tccgtcatgg atggcaaact gcataagcca
     3121 taaaaaaaca gggagacatt tatgaagctt cgcctgtcgg cgcttgctct gggaactacg
     3181 cttctggtgg ggtgtgcgag ttccggtaca gatcagcaag ggcgttctga cccgttagaa
     3241 gggttcaacc gcaccatgta caacttcaac ttcaacgtat tagacccgta tattgttcga
     3301 ccggtcgctg tcgcctggcg tgattatgtt ccgcaaccgg cgcgtaatgg tttgagcaac
     3361 tttactggca accttgaaga acctgcggtg atggttaact acttcttgca gggcgaccct
     3421 tatcagggga tggtccactt tacccgcttt ttcctgaaca ccattttggg gatgggcggc
     3481 tttattgatg ttgcagggat ggcgaacccg aaactgcaac ggactgaacc tcaccgcttc
     3541 ggtagtacgc ttggtcatta tggcgtgggt tatgggcctt acgttcagtt accgttctac
     3601 ggtagcttca cgctgcgtga tgacggtggt gatatggcgg atggtcttta cccggttctt
     3661 tcctggctga cctggccgat gtctgtgggt aaatggacgc ttgaagggat cgaaacccgc
     3721 gctcagctgc tggattccga tggtctgctg cgtcagtcgt ccgatcctta tattatggtg
     3781 cgcgaagcgt acttccagcg tcatgatttc atcgctaatg gcggcgaact caaaccgcag
     3841 gaaaatccga acgcacaagc gattcaggat gatttaaaag atattgattc tgaataagaa
     3901 acaaataaaa aaggtgagtc gcaaaactca ccttttttgt tgttatccct caattaccac
     3961 aagtaaaagg agataaaccg a
//
LOCUS       DAZKHC020000216         3965 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZKHC020000216 DAZKHC020000000
VERSION     DAZKHC020000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.217"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            609..1112
                     /locus_tag="ACHOSM_RS23950"
                     /old_locus_tag="pgaptmp_004790"
     CDS             609..1112
                     /locus_tag="ACHOSM_RS23950"
                     /old_locus_tag="pgaptmp_004790"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU4561117.1"
                     /translation="MQDFNICCRRDNHYPEHKLIPLSELFDVNYGLNLELNKLEKDSS
                     GINFVSRTSKNNGVSARVKLKDGISPLPAGVLTVAAGGSVMESFVQDSPFYSGRDLYW
                     LRPKVELTLEEKLYYCSCIRRNRHKYSYGRQANRTLKNLLVPSLDSVPAWVYGVTGKI
                     ISELSER"
     gene            complement(1160..1891)
                     /gene="traT"
                     /locus_tag="ACHOSM_RS23955"
                     /old_locus_tag="pgaptmp_004791"
     CDS             complement(1160..1891)
                     /gene="traT"
                     /locus_tag="ACHOSM_RS23955"
                     /old_locus_tag="pgaptmp_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HGU4561118.1"
                     /translation="MKTKKVMMIALVSSALALSGCGAMGTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKARGYQIVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLSRGYEGAAVGAALGGGITAYNTGSAGTTLGVGLATGLIGMAADAMVE
                     DINYTMITDVQIAERTRTSVRTDNVAALRQGTSGSKIQTSTETGNQHKYQTRVVSSAN
                     QANLKFEEAKPHLEDQLAKSIANIL"
     gene            2316..2579
                     /locus_tag="ACHOSM_RS23960"
                     /old_locus_tag="pgaptmp_004792"
     CDS             2316..2579
                     /locus_tag="ACHOSM_RS23960"
                     /old_locus_tag="pgaptmp_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGU4561119.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            2881..3021
                     /locus_tag="ACHOSM_RS23965"
                     /old_locus_tag="pgaptmp_004793"
     CDS             2881..3021
                     /locus_tag="ACHOSM_RS23965"
                     /old_locus_tag="pgaptmp_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4561120.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVRFMWSEIAAFLQC
                     KP"
     gene            complement(3888..>3965)
                     /locus_tag="ACHOSM_RS23970"
                     /old_locus_tag="pgaptmp_004794"
     CDS             complement(3888..>3965)
                     /locus_tag="ACHOSM_RS23970"
                     /old_locus_tag="pgaptmp_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561121.1"
                     /translation="IDNYISKIKDNHNTPWLLNNENVQE"
BASE COUNT         1048 a          788 c          932 g         1197 t
ORIGIN      
        1 gaaatagtat gtttctcatc gaaagcaagt ttgtcaatat aaatttgtaa cagaaatgac
       61 tttccgggga taaacaaaac tgttcatggc aatggctttc tctgtcgcca acatggatac
      121 atcgttccgg ataaagcact gatgccattg actgacgcag actatcaggt catctgatgc
      181 tttcttttcc ttcaccggta ttgatgatat gccttatgaa gggggactta ttcctgtaac
      241 ccaatcacag gcgctgtgat tactggttta tgattgatgc tgatttgagc agatatgcac
      301 agcagttgtg gattcagggg atattttctg ccagatattc ataccagatt gatgacctct
      361 ttgctcagat gctcttcgtt ctgagccagc caggattttg tggccacata cattgttcgc
      421 cgatggggaa tcgagacagg cgattggctg tgatgcgatt ttgccgctaa ctgaggatga
      481 gtccgcccct cccggggaat tcctgtaagt taagccgtac ctgttgatta gcactaaact
      541 gggtaatgaa tttatctgcc agtatgaatg atgccgggga cggttttgaa ctcctgatgt
      601 cgggtaatat gcaggatttt aatatttgtt gtcgtcggga taatcattat cctgaacata
      661 aactgatacc tttatcagag ttatttgacg ttaattatgg actgaatctt gagctgaata
      721 aactggagaa agattcttca ggaattaatt ttgtttcaag aacgtccaaa aataatggcg
      781 tctccgccag ggttaaatta aaagatggta ttagtccatt gccagcaggt gtgttaactg
      841 tagcagcagg aggttctgtt atggaatcgt ttgtacagga ttcaccattt tatagtggaa
      901 gagatcttta ttggttacgt cctaaagttg aactgactct ggaagagaaa ttgtactact
      961 gctcttgtat aagaagaaac agacacaaat acagctacgg gcgtcaggca aaccgaacgc
     1021 tgaagaattt actggtaccg tcattagatt cagttccggc atgggtatat ggtgtgacag
     1081 ggaaaataat atctgaattg tctgaacgtt gagactaaga gggccggagg ccaggtgaat
     1141 ggcctccggg atatttatat cagagtatat tggcgattga ttttgccagc tggtcttcca
     1201 gatgaggttt agcttcttca aacttcagat tagcctggtt ggcactggat acaacccggg
     1261 tctggtattt atgctggttg ccggtttctg tgcttgtctg aattttggag cctgatgttc
     1321 cctgacgcaa agctgcaaca ttatccgttc tgacgctggt tctggttcgc tcggcaatct
     1381 gaacatctgt aatcattgtg taatttatat cttccaccat tgcatccgcg gccatgccaa
     1441 tcagaccggt agccagtcca acaccgagag tggtacctgc cgacccggta ttgtacgctg
     1501 tgattccacc tccaagagca gctccgactg ctgcaccttc ataaccgcga ctcagccatc
     1561 cctgagattc acgcagatcc attttgtctg ctttgagaac gttagcctgg atccagtagt
     1621 aagctttatc cggggaagtc acgatctgat aacctctggc tttgactgca tcggcaattt
     1681 ttccctgtag tccactcatg tctttatccg acgtgttttt gatctgcagg aaaacggtgc
     1741 gttcgctcgc aggttcaagc cagatggttt cactcatctg ggtttttact tccagatttc
     1801 gttttttgat cgctgtgccc atggctccac atcctgaaag agccagcgca gagctgacca
     1861 gtgcaatcat cataactttt ttggttttca tttaatgccc ttcttatccg acaggtttat
     1921 ggaagacaga gaaaccgcct ttacggtata ttttttcagc gggcttctca agtatgttaa
     1981 aggatacagg taaaaacagc gggatcagat acgtcgggta aacgtttatt tcataagaag
     2041 tttttaggtc agaataaaat aatgggtgtt gttatattat cagatgatga tttatgtgag
     2101 cgcgttacgg atattgcaca tgaggttaaa ggagagagca agcctccgca acgcctcgct
     2161 tcttatttaa tggatttttt cgggaagtcc agtttataat tatataacat attgtaatac
     2221 tgaaagagag tgaggttata agaacagaca gttgaatctg accgtctgaa tatgtacctt
     2281 tatgcggaat tttcacttaa agggggaaaa ctgttgtgaa accgaatatc catccggaat
     2341 accgtacagt agtatttcac gataccagta ttgatgagta cttcaggatc ggttcgacga
     2401 tcaaaactga ccgggtaatt gaactggatg gggtaactta cccatatgtt acaattgacg
     2461 tttcttctaa gtcgcatccg ttttatacag gtaaactgag aactgtcgct tcagaaggta
     2521 atgttgcacg ctttacccag cgttttgggc gttttgttga tgcgaaaaaa ggtggctgat
     2581 tttgccgcca gggtgcgttt gacaatttaa aaacgcaccg gacagagaga tcctgcacct
     2641 gaatataccg ttagcccgtc ccaccggggc gtgtgcagat gatctcgtca ccggaagatt
     2701 gcggacctct ctgaaaacca gtacatcacg cgcgggtgcc tttcggctga cggctggaag
     2761 gattacctga aggccggatg tggaaaggcc ccgagtcaac ttaaacgtta acccgaggcc
     2821 ctgaccatct accttcagca agtggaaggt tagcgcctct ctgtaaaagg agcaagtgct
     2881 atgccgcaaa aaacgatcat tgtgggcatg ttatgcctga ccatgttgct gaccgtctgg
     2941 gtgttacacg catcaccgtg tgaattcagg gtacgcttca tgtggtcgga aattgcggcg
     3001 ttcttgcagt gtaaaccgta agaaaccgca gcggggacaa cagtccccgc tatccggttg
     3061 ccaaaggttc ggtcgaaagt cgcacccttg cacacaaatc acaaagaaaa aaaagtccgc
     3121 agtgtaaaaa ctgtgggctt ttttattcgc atcccgaaag tgtggtaaca gcgtgggggc
     3181 accaaaaaaa gccccataaa atggggctga agattctgta aggaatttca cagcaatgag
     3241 attcctgctt tctccaggga tatacgtgtc ttcaggctga atcaatgggg gcgtggctct
     3301 gaatccacct gcgattaaaa cttgcacaat gccgggagtt gagttagaaa agtacagggc
     3361 gcgatatccg gcttattttg ttgctgtgtt ttgcctcgtg ctggatgtcg cgtccaccgt
     3421 ttttctgatg attcatatga aatggtactt tattgattta ctgccattct gatttgcctt
     3481 tttatgttca ttattgcggt tgtcacttcc agtttctagt gatgttttga taaaagtgaa
     3541 agaataaaac gataattgca gaaggtattg ctaaaactat acatatcatt atataaacaa
     3601 gatgaaaatg atatatacca taagaccaga aaagaggttt tttccagaat gatatggatg
     3661 tgtattttag tgtgattgtt tttgtcaggt atattgaaca aggaagggcc aggccactga
     3721 gtatcagaaa taagtcaact tttatcgatg cttctgttgg tgtgtcatat atcagatgca
     3781 atatggatat tataatgggg cacattcccc acaggatatt tttgaaatag aatttccggt
     3841 tcatatgttt atgctctttt gttttatctc aggaattgtc tcttttttta ttcctgaaca
     3901 ttttcgttgt ttaataacca tggggtatta tgattatcct ttatttttga tatgtaattg
     3961 tcaat
//
LOCUS       DAZKHC020000217         3943 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZKHC020000217 DAZKHC020000000
VERSION     DAZKHC020000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.218"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(43..288)
                     /locus_tag="ACHOSM_RS23975"
                     /old_locus_tag="pgaptmp_004795"
     CDS             complement(43..288)
                     /locus_tag="ACHOSM_RS23975"
                     /old_locus_tag="pgaptmp_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561122.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKKGALEV
                     AREAACRLRDMGWTPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(288..1238)
                     /locus_tag="ACHOSM_RS23980"
                     /old_locus_tag="pgaptmp_004796"
     CDS             complement(288..1238)
                     /locus_tag="ACHOSM_RS23980"
                     /old_locus_tag="pgaptmp_004796"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGU4561123.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFEDPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQQGIQKGIQQGIQQGIQQGIQQGIQ
                     KGRMEEKQDIARQLQKTGMPSAQICQITGLSEAELKNITH"
     gene            complement(1303..1569)
                     /locus_tag="ACHOSM_RS23985"
                     /old_locus_tag="pgaptmp_004797"
     CDS             complement(1303..1569)
                     /locus_tag="ACHOSM_RS23985"
                     /old_locus_tag="pgaptmp_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561124.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRVRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(1660..2094)
                     /locus_tag="ACHOSM_RS23990"
                     /old_locus_tag="pgaptmp_004798"
     CDS             complement(1660..2094)
                     /locus_tag="ACHOSM_RS23990"
                     /old_locus_tag="pgaptmp_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HGU4561125.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQLA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            complement(2766..3266)
                     /locus_tag="ACHOSM_RS23995"
                     /old_locus_tag="pgaptmp_004799"
     CDS             complement(2766..3266)
                     /locus_tag="ACHOSM_RS23995"
                     /old_locus_tag="pgaptmp_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGU4561126.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPVNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDTFRYAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(3681..>3943)
                     /locus_tag="ACHOSM_RS24000"
                     /old_locus_tag="pgaptmp_004800"
     CDS             complement(3681..>3943)
                     /locus_tag="ACHOSM_RS24000"
                     /old_locus_tag="pgaptmp_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561127.1"
                     /translation="LDADDENLPVVLDAILNRGARFSAVEMYLVSECVEHILSSGLAC
                     DVLRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRG"
BASE COUNT          846 a         1188 c         1093 g          816 t
ORIGIN      
        1 gggaatgtgt gagcatgatt cacagcctgg cagaatgcca ggctactgct catcaggaaa
       61 cagctttttc agttcttcac cggaaagtcc ggttgcctcc tgaatccgtt ccggcgtcca
      121 gcccatatcc cgcagccggc aggctgcttc ccgcgccact tcaagcgcac ctttcttaaa
      181 accttcgtcg aaacctttct gtataagacg ttctgcaatc gtcatgctgt ttgctctgct
      241 cctgctgaca aaaaaacccg gcattttgcc gggctttgtg gttctcatca gtgagtgatg
      301 ttcttcagtt cagcttcgga aagtccggtg atctgacaga tttgtgcaga tggcattccc
      361 gttttctgaa gctgacgcgc aatatcctgc ttctcctcca tgcggccttt ctgtattccc
      421 tgctgtattc cctgctgtat tccctgctgt attccctgct gtattccttt ctgtattcct
      481 tgctgctcaa gacgttcggc aatagtcatc agtttatcct catgctgcgg cagacgctca
      541 gccagcgccc gcataaattc cggtgataca gagtcccctg cgttaacgat gtaatgaatc
      601 agaacacgca cctgctcgtc tgaaatttcc accatgacct gcggaagttt gtccagcagc
      661 tccatcatgt cacgatggcg gatatgcttc atcagcagcg tcagtgcggc catccggcga
      721 tggttcatga tttcattgtc atcaacgaca gtgatatcaa ccagcggaaa cggctttgtg
      781 tatattttag ccgcaagcgc cggatcctca aaacagtcca gccagttcat gctgtacggg
      841 tacgggctgc gctcaccatg atagaacagc accggaatca ccagtggcaa tgttttgtgc
      901 tgctccagat gtctctgcat cgccgccaca ctgtaacgta acatgcggaa aggcatgtac
      961 agattttcgg tgctctggtg ctcgatgagc gtataaacat agccgtcttc acccccggtg
     1021 gttttcacac tccagaggat atcgctggcg tactgatgca ggtccggctc aacaaaggtg
     1081 gcgggttcca gcttcagcgt ggacaggtcg cacaactgcc ggtactccgc cggaagatgc
     1141 agttccagaa aatctctggc gacgtcggga ttcgccagga acgaccggaa cgcggcatca
     1201 tgcggcgtgg gcgttgtggt gtttttcttc ttactcacgg ttcctcctgg tgagatgtct
     1261 gaacgtatcc tgccgcctgc acaggcagac ggcaagggtt aatcagtggc agacccgttc
     1321 aatttcaagc gcgcggagca ggtggcccca ttcgctgact ttcatggcgg ggatacgtac
     1381 ccgcttctgc tcactgcagc gtctggcgat atgaccgctc atcgccagga cgtccgggtt
     1441 aatctcatcc tgtgccagct cctgacgtct gacgcgggta tggcggtgca ggggtttatt
     1501 ctcccgcagg cactcttcac gcagacgctt tacatattca gccgttgcaa agataattga
     1561 tggtctcaca aatatttctc cttatgttgt tgatgactgc cgctcttcct ggccgggaag
     1621 cagcggcgga gagaggcagg gtttatcgcc ctgctttttt cattccgggc agcggcgcag
     1681 tgccagcgcg agcgctttca tcttccagcc ttccggcgtg cggtacatca ccttccagta
     1741 gccgtcgatg aacacataat ggaagcgcac ctcttctgtg ctttccgtgg caatagcctc
     1801 catgaaagaa cggtactcac gcgcctcact gtccatccgg aaaccactgt cacgtccgta
     1861 ataagccgtc acaccgtcca ccgggttctg aagcgtatgc ccggcgggtt tgtcgcaacg
     1921 gggaccgagg caacggatat ctccgtgttc aaccagggct tcggccagct gaccggtggc
     1981 atagtgggta tgcaggatgc gaccgttcca ggtcggctca ccgtgcaggt ggcagtaaac
     2041 ggtacggaac accccgtcgc ggcaaaaaac ggaaatcgtg ctgctgactg tcatgaaaac
     2101 gtctccttct gctgaatcag gtcgccgggc cgaccggttg ctcccccgga ggggcgaaag
     2161 ccccttctgc cgtccacttc ttcggggttt ctccccgcag tggacctttc cgatggggca
     2221 gcgacagcca cggcacgggg cagttgtcca ggggcagacg caaaagtttt tgacgcttgc
     2281 ggcaaaaagt tttgtggctg gtacatctcc ccctggacgg ctgacacgtg ttgtggccgc
     2341 ttcagccccc ggaaagtcca cagcgggaaa cagaccgcag gggtggcggc ggaagggttg
     2401 actgcgccat caggcgcagt ggccttacac accgtcaggt gtgcagggag cgccgtttcc
     2461 ggcgcgacag gggcggcgcg accggaacgg tcgctgcaag cctggttttt cagacatcgg
     2521 accgtagcgg cagcccgaaa ccgcgcctgc cgaccggatg ccgccaggca gccgggagcc
     2581 gaaggtgtcc ccgcctccgg aaatcagaac aacagaaacg acataacaga aaaatgcctc
     2641 acaccatgac gggcaggaag ggcagcgccg caggcgcggc cctcctgcat ttcaggcagg
     2701 cgtaagcctg ccagggtttt ccggcggcct ggcaggccat acccccgccg gagcggggga
     2761 gtaaatcacc gacggaacac atgaccgtca ataaaggtga aggagtccag gaataaatcc
     2821 cgcgcatacg cctcatagtc aaaatagagc gccaccgtct ccggcacgtc agccagcagg
     2881 ccggtatcac tggcgaactc aaccgcaaaa gcctcctcac tgtcagcctc gccccaccag
     2941 gcatagcgga aggtgtcaaa atccgtctca ccggtatcat ccacccagag acgataagcc
     3001 tcttcgcagc cttcatcccg cgcctgtcgg aagccttcaa cccaggccca gttgatatgg
     3061 cattcggagg ccatattcac cgggaacccc tcataatcct gaaacatcag ttcaggatcg
     3121 gcttcatcct ggtgaagagc acggcaggcg gcgaaaaaat cgcgttcatc atcaaacgtg
     3181 gtcagatcaa accagcgtcc ggcgatgctt ccacagttgt atttgtgcca ggttccaacg
     3241 tatacagcag gtgcaacaac agacattccc gaatcctcca tagtcacatg ttctgcggca
     3301 ccgggcggtg ccgtgcctcc ataaaagccg ggcgataagg cggggatgca cagggcgcaa
     3361 gggaagccgc agcctgacgc acacggctgg cgggagttgg agggtgagac cgcgaaatct
     3421 gagcgggtga acgaccggaa aatcacggca gacgggaagg agggaccggg tgaccgcagc
     3481 ggcgaagcgt acccttgcat caacgccagc ccggctaaga tggaggcacg gcacagcccg
     3541 cacacgacgc tgaggccaca gagctgaaaa cccacaaggc catgccccgc atggccgggt
     3601 aaaagcggag ccgccggcga cttcctttgt gctctgtccg gggaccggca gaaaaaaagc
     3661 ggggattttc cccgccatca tcagcccctg atttttgcca gggcatccgc catgctgcgg
     3721 atttcggccc gggcttcccg gaccacctcc cgcagaacac cacggtcaaa ccaccggcgg
     3781 ggcggttcat caggtatacg cagcacatcg caggccagcc cactggacag gatatgctcc
     3841 acacactcac tgaccagata catttccacc gccgaaaaac gtgctccccg gttcaggatg
     3901 gcatcgagca ccacgggcag gttttcgtcg tctgcatcga gcc
//
LOCUS       DAZKHC020000218         3940 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZKHC020000218 DAZKHC020000000
VERSION     DAZKHC020000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.219"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(72..2105)
                     /gene="metG"
                     /locus_tag="ACHOSM_RS24005"
                     /old_locus_tag="pgaptmp_004801"
     CDS             complement(72..2105)
                     /gene="metG"
                     /locus_tag="ACHOSM_RS24005"
                     /old_locus_tag="pgaptmp_004801"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGU4561128.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGHDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2237..3346
                     /gene="apbC"
                     /locus_tag="ACHOSM_RS24010"
                     /old_locus_tag="pgaptmp_004802"
     CDS             2237..3346
                     /gene="apbC"
                     /locus_tag="ACHOSM_RS24010"
                     /old_locus_tag="pgaptmp_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HGU4561129.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3609..3890
                     /gene="yehE"
                     /locus_tag="ACHOSM_RS24015"
                     /old_locus_tag="pgaptmp_004803"
     CDS             3609..3890
                     /gene="yehE"
                     /locus_tag="ACHOSM_RS24015"
                     /old_locus_tag="pgaptmp_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HGU4561130.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT          964 a          980 c         1011 g          985 t
ORIGIN      
        1 aaaatcgcaa gaaaacaact ttaggaattt ataaagaaaa ggcgctgtcg atgcagcgcc
       61 ttgaaggggg attatttcac ctgatggcct ggtttagcac cagcatccgg gcttaacagg
      121 aagatatctt tcccgccagg accagcagcc attaccatgc cttcggagat accaaagcgc
      181 attttacgcg gtgccaggtt ggccaccata atggtgtgac gaccaatcag tgcctgcgga
      241 tccgggtagg cagaacgaat accggagaag acattgcgtt tttcaccgcc gagatccagt
      301 gtcaggcgca gcagtttgtc agaaccttca acaaactctg cgttttcgat cagcgccacg
      361 cgcaggtcaa cttttgcgaa gtcgtcaaag gtgatggttt cctgaatcgg atcatctgcc
      421 agtgggccag taactggcgc ggcagctgct ttcacttctt ctttagacgc ttccaccagt
      481 gcttcaacct gcttcatatc gatgcggtta tacagcgctt tgaacggatt cactttgtgg
      541 cccagcagcg gttgctggat accatcccag gtcagttctg tattgaggaa tgcttctgcg
      601 cgctcggtca gtttcggcag taccggcttc aggtaagtca tcagcacgcg gaacaggttt
      661 atgcccattg agcaaattgc ctgcaggtcg gcatcgtggc cttcctgttt tgccaccacc
      721 cacggagcct gctcgtcgac atagcggtta gccagatcag ccagcgccat gatttcgcgc
      781 actgctttac caaattcgcg gctttcccac gcttcaccaa tcacttcagc ggcatcagtg
      841 aaggttttgt acaactgcgg gtcagccagt tcgcttgcca gcacgccgtc aaaacgcttg
      901 ttgataaagc ccgcattacg agaggccagg ttaaccactt tgttcacgat atcggcattc
      961 acacgctgaa cgaaatcctc caggttgaga tcgatatcat caatgcgcga agagagtttc
     1021 gcagtgtagt agtaacgcag gctgtcagcg tcaaaatgat tcagccaggt gctggcttta
     1081 ataaaggtgc cgcgagactt ggacatcttt gcgccgttca ccgtcacata gccatgaaca
     1141 aacaggttgg tcggcttgcg gaagttgctg ccttccagca tggcaggcca gaacaggctg
     1201 tggaagtaaa caatatcttt accgatgaag tggtacagct cggcggtgga gtctttcttc
     1261 cagtattcat cgaagcttac gctgtcgccg cgcttgtcgc acagattctt gaaagaaccc
     1321 atgtagccaa tcggtgcgtc cagccagacg tagaaatatt tgcccggcgc gttcggaatt
     1381 tcaaaaccaa agtatggcgc gtcgcgggag atatcccact gttgcaggcc ggattcaaac
     1441 cactcctgca ttttatttgc cacctgctcc tgcaacgcac cgctgcgggt ccatgcctgc
     1501 aacatttcgc tgaaagaggg cagatcaaag aagaagtgtt cagaatcacg cattaccggc
     1561 gtagcgccag aaaccaccga tttcggctcg atcaattcag tcgggctgta ggtcgcgccg
     1621 cagacttcgc agttatcgcc gtattgatcc ggggatttac atttcgggca ggtgcctttc
     1681 acaaaacggt ccggcaggaa catgcctttt tccggatcgt acagctgaga gatggtgcgg
     1741 tttttaataa aaccgttctc tttcaggcga gagtagataa gttctgacaa ctggcggttc
     1801 tcttcgctgt gcgtcgagtg atagttgtca tagctgatgt taaagcctgc gaaatcagtc
     1861 tgatgctcct gactcatttc gccaatcatc tgctccgggg tgataccaag ctgctgagct
     1921 ttcagcatga tcggtgtacc gtgggcatcg tcggcgcaga tgaagttgac ctcgtggccg
     1981 cgcattcgct ggtaacggac ccagacatca gcctggatgt gctccagcat atggccgagg
     2041 tggattgagc cgttagcgta cggcagtgcg cacgtcacca gaattttctt cgcgacttga
     2101 gtcatagtag gcattacttc ttaatagtga aaaggggtta gatagtacca aatgggaaaa
     2161 tgttaagtaa gtacttctct atattcttca ggtaaactat cagctgatgg tttatctata
     2221 caaaaaagga gtcgggatga acgaacaatc ccaggccaaa tcaccagaag ccttgcgcgc
     2281 aatggtcgcc gggacactgg ccaattttca gcacccaacc ctgaagcata acctcaccac
     2341 gctgaaagcg ttgcaccatg tcgcctggat ggacgacacg ttgcatgtgg aactggttat
     2401 gccattcgtc tggcatagcg cgtttgaaga gttaaaagag caatgtagcg ccgaactgct
     2461 gcgtatcacc ggggcgaaag ctatcgactg gaaactgtcg cacaatatcg ccaccttaaa
     2521 acgcgtgaag aatcagccgg gtattaatgg tgtgaagaac atcattgcgg tcagttccgg
     2581 taaaggcggc gtgggtaaat cctccacggc ggtaaacctg gcgctggcac tggcggcgga
     2641 aggggcgaaa gtcggcattc tggacgctga tatctacggc ccatcaattc ccaccatgct
     2701 gggcgcagaa aatcagcgtc caacttcgcc ggacggtacc catatggcac cgattatgtc
     2761 tcatggcctg gcaaccaatt caattggtta tctggtcact gacgacaatg cgatggtgtg
     2821 gcgtggaccg atggcgagca aggcgttaat gcagatgttg caggaaacct tgtggccgga
     2881 tctcgactat ctggtgcttg atatgccgcc aggcaccggt gacattcaat tgacgctggc
     2941 gcaaaacatt ccagtaactg gtgcagtcgt ggtcaccacc ccgcaggaca tcgcgttgat
     3001 cgatgcgaaa aaaggcattg tgatgttcga gaaagtcgag gttccggtgc ttggtattgt
     3061 cgagaacatg agtgtgcata tttgcagtaa ctgtggtcac catgaaccga ttttcggtac
     3121 cggtggtgca gagaaactgg ctgaaaaata ccacacacag ctgctggggc agatgccgtt
     3181 acacatctcc ttacgtgagg atctcgataa aggcacgcca acggttatca gccgtccgga
     3241 gagcgaattt accgctatct accgccagct ggctgaccgc gttgcagctc agctctactg
     3301 gcagggtgaa gtcattccag gcgagatttc cttccgcgcg gtctaacgcc tattaaccat
     3361 tctggttatt cttcatacac cgcagagatg ttacattgat gcggtgtatt agtttttgcc
     3421 gcattaatga acacttattt aatactgcga atattctgcg gaccttcgtc cgaaactcta
     3481 caaagtcgat tgatcataca ggcaatgctt cattatcatc cttcgtggct gtgtagtgga
     3541 tgtacagcta tagtttgtta tgcactcatt ctatccaggc ataaccttta ctacagggat
     3601 aacgttcaat gaataagtat tggctgtcag gaattatttt tcttgcctat ggattggctt
     3661 ctcccgcagt ctcttcagag actgccacat tagcgattaa tggcaggatc agtcccccaa
     3721 catgcagtat ggctatggtt aatggtcaac ctcaacagca ttgtggtcag ttaacgtata
     3781 atgttgacac ccgtcatcag gtttcttcgc ccgtcaaagg tgttacgaca gaagtggttg
     3841 ttgcaggtag cgatagcaaa cgtcgaatcg tgttaaatcg ctacgattaa tttggcaacc
     3901 tgattttaat tgcatttaga aagacgaaat catttatata
//
LOCUS       DAZKHC020000219         3903 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZKHC020000219 DAZKHC020000000
VERSION     DAZKHC020000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.220"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..195)
                     /gene="escO"
                     /locus_tag="ACHOSM_RS24020"
                     /old_locus_tag="pgaptmp_004804"
     CDS             complement(<1..195)
                     /gene="escO"
                     /locus_tag="ACHOSM_RS24020"
                     /old_locus_tag="pgaptmp_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HGU4561131.1"
                     /translation="MAKINSQIKEVDGKLDDCEQAIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKK"
     gene            complement(255..1595)
                     /gene="escN"
                     /locus_tag="ACHOSM_RS24025"
                     /old_locus_tag="pgaptmp_004805"
     CDS             complement(255..1595)
                     /gene="escN"
                     /locus_tag="ACHOSM_RS24025"
                     /old_locus_tag="pgaptmp_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HGU4561132.1"
                     /translation="MILEHDSVLERYPRIQKVLNSTVPTLSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHISGMYCGQWLSYQGEEF
                     KIRVGDELLGRLVDGIGRPMGSNITAPYLPFERSLYAEPPDPLLRQVIDQPFTLGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PLSTLSKCVLVVTTSDRPALERMKAAFTATTIAEFFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVKSEHLRAAAECKKLIATYKN
                     IELLIRIGEYTMGQDPEADKAIKNRKAIQSFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1579..3606)
                     /gene="escV"
                     /locus_tag="ACHOSM_RS24030"
                     /old_locus_tag="pgaptmp_004806"
     CDS             complement(1579..3606)
                     /gene="escV"
                     /locus_tag="ACHOSM_RS24030"
                     /old_locus_tag="pgaptmp_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HGU4561133.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMIVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLTDDTVYFEAGHAEISFEFVQEKLSANSIVYKTNEAN
                     QQLAHVSGMDVYAKTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENISIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVRGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERSEQIIGFLKNIVNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            complement(3603..>3903)
                     /locus_tag="ACHOSM_RS24035"
                     /old_locus_tag="pgaptmp_004807"
     CDS             complement(3603..>3903)
                     /locus_tag="ACHOSM_RS24035"
                     /old_locus_tag="pgaptmp_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561134.1"
                     /translation="DFYLFDNRVEIDFNGFSFFIEIIDSNVFITFALEYNENAFFSFF
                     NALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFSLMNTFQQHVQIIERVRRMTS"
BASE COUNT         1217 a          910 c          701 g         1075 t
ORIGIN      
        1 ttttttctca tataatcgct gcttttgctc atcaaatgca ttgtttttaa tctggtattt
       61 atacaacgac actttatcca gattcaccag cgatgcacaa tacgcctgct tagaggcaat
      121 cgactcttta atggcttgct cgcagtcatc gagtttacca tccacctctt ttatctgaga
      181 gttaatctta gccattgatt ctctcaatcg attcgctctg cttttacgaa tagataaaat
      241 tctgtccaac atattcaggc aaccactttg aataggcttt caatcgtttt ttcgtaacta
      301 ctgatatctt tggtgctttg ttgtataaag ctctgaattg ctttcctgtt ttttattgcc
      361 ttatccgctt caggatcttg ccccatcgtg tactcaccaa tacgaataag cagttcaata
      421 ttcttatagg ttgcaataag ctttttgcat tcagccgctg cacgtaaatg ttccgattta
      481 acaacgttat gcataacacg actggcgctt aaaccaatat caattgcagg gaaatggttt
      541 tcctctgcaa gttctcgggt aagcacaata tgtccatcaa gaatagagcg aacttcatca
      601 ccgatgggat cattcacatt atcgctttct aacagcaccg tataaatcgc agttattgaa
      661 ccttttggcg caggtcccgc acgctctaat aatttgggta acgaggaaaa aacactcgga
      721 gggaatcccc cccttacatc aggctctccc gatgcaagcc caacatcacg cgcggctcgg
      781 gcataacgag ttacggaatc catcattaat aatacatttt tgccctgatc ccggaagaac
      841 tctgcaatgg ttgtcgccgt aaatgcggcc ttcattcttt ccagcgcggg acggtctgac
      901 gttgtgacga ccagtacaca tttagaaagc gtggatagag gtaaaagcgc gaggaattca
      961 tttacttcgc gaccacgctc gccgataagg gcaaggacaa taatatccgc ggatgcgcca
     1021 ttacaaatca ttcccaaaag cgtactttta ccaacgccgg accccgcgaa aataccgatg
     1081 cgttgcccaa taccgcaagt aagtaaacca tctattgccc tgacgccaag cgtaaatggc
     1141 tgatcaataa cctgccttaa taaaggatcg gggggttcag catacaaact gcgttcaaac
     1201 ggaagataag gtgcagtaat attgctcccc attggtctgc ctataccatc aaccagtcgc
     1261 ccaaggagtt cgtccccaac acgaatcttg aactcttccc cctgataact tagccactgg
     1321 ccacaataca tgccggaaat atgctcaaaa ggcagcagga aaacctcatc ttcatcaata
     1381 gcgatcacct cagctaaacg ttgactgggt tctatcttgt aaaaggcgcc aatacgcgct
     1441 tttggtaaac gcgccttgat aatcgtcccg ccgatattgg taatcttgcc ttcatatctg
     1501 gtagacgaat ttaatgataa tgtcggcaca gtgctattga gcactttctg gatacgtggg
     1561 tatctttcca atacagaatc atgctctaaa atcatttacc gttcctaata ctttaagttc
     1621 gatattattc ccaacttcct gaaaagagag cacggggact gacgggaacg aaccttcaat
     1681 cattttcttc acatagcgcc tgatatctaa agcggtcaga atgacgccat tccccgttgg
     1741 attcacaata ttttttaaga agccaataat ctgctcactt cgctccggtg agatattcaa
     1801 atacgaacct gatgacgttt gtctgatcga ctctcgtaac tcattttcaa tatcagagcc
     1861 aataagccag acattcagaa gtgtaccgcg aacgctatag cgacctaaaa tatgccgacg
     1921 gagggcgata cgaacgtatt cgcacaagat aaccacatct ttttcttttg tggaccaaaa
     1981 aataagcgtt tcaaaaatag ttcgtagatc tctaattgag atattttcct ctacgagacg
     2041 ctgaagaata tccacaatct tgctcagacc aagctgacgc tgaagctctt tcacaagttc
     2101 gttatatttc ctctccatga tgtccatcag ataacgcgtt tcttgtacgc cgatgaactc
     2161 tttggcatta gacaatacaa gtttctttag caaaaacgtt atcttgtcat ttgtttttgc
     2221 ataaacatcc atacctgaga cgtgagctag ttgttggtta gcctcattcg ttttatacac
     2281 gatagagtta gccgaaagct tttcctggac aaattcaaat gatatctctg catgcccagc
     2341 ctcaaaataa acagtatcat ctgtaagagt atctttgtat attgactctt gatagagcaa
     2401 aaattctata gcgctatcat tatttttaac tggattaaca ataatcttag gcaatggaat
     2461 tccactttcc tcaaagaaat tccaacgcat aacctcaatc tgctgtgtaa tatctgagct
     2521 ataaatatta ctacttaagt ttaaaatcaa cggctcggca cccggtgaga tattttgact
     2581 attagacaaa tcagctccca tagcgtcctt attatttccg gttccaaacg ttcttttctt
     2641 ttgtaatttc caggcgatac ccaacaggca aaccgccaga aacaagaaga ccaatgtagg
     2701 aaagccagga ataatcgcca ttacgatcat gagtgcggca ctgattaaaa aagggcgact
     2761 attaacagaa acctgttgaa caatatcacc tgcaaggttt tcttcttttt cgctttcacc
     2821 gggaacacga gtaacaacaa cccccgcagt gacggaaata ataagcgcag ggatctgggc
     2881 aaccaaagca tcaccgacag acaatacaga aaacaggcta agtgcttcac taaatggcat
     2941 gtcaaattgc cacattccaa tgagcacgcc accaaacagg ttaactaaaa caatgatgat
     3001 accggcaata gcgtcccctt ttacaaactt catcgccccg tccattgaac cataaaactg
     3061 gctttccttt tgcactcggg agcgaagaac ttttgcctca agcggatcaa taacgcctgc
     3121 gcgcatatca ccatcaatac tcatttgttt tcctggcata ccatcaaggg aaaaacgagc
     3181 actcacttca gcaacacgct cagccccttt tgtaataacc ataaattgta caatggtgat
     3241 aatggtaaaa ataaccaggc caacaacaat attaccgcca acaacaaagt taccgaagga
     3301 gtaaataatg tcacccgcat cgtgatgcag cagaataagt ctggttgtac taacactgag
     3361 cgacaggcgc atcaacgtcg taatcaacag gattgtagga aaagaagtta gctcaagagg
     3421 attttttata tagattgaga gcattaataa aagcaacgct gtcgatatat taatcgcgat
     3481 aatgatatcc accacaactg ttggcaatgg aataatcatc atcattacag ccataaagaa
     3541 gaagagagcc agaataagat cgtgatatga ctctgctttt ttaaatatat ttaagagttt
     3601 attcatgatg tcatcctgcg aactcgttca ataatctgca catgctgctg aaaagtattc
     3661 attagagaaa acacatcaat attggttaac aaacaactta agcacaattt gttatcgtag
     3721 acaaaaatat gttcaatcac tccctgcgtc cgttcagggg caagggcatt gaaaaaagaa
     3781 aaaaacgcat tttcgttata ctctaaggca aacgtaataa acacattact atcaattatt
     3841 tcaataaaaa aagaaaaacc attaaaatca atttccacac gattatcaaa caaataaaaa
     3901 tca
//
LOCUS       DAZKHC020000220         3902 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZKHC020000220 DAZKHC020000000
VERSION     DAZKHC020000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.221"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..426)
                     /locus_tag="ACHOSM_RS24040"
                     /old_locus_tag="pgaptmp_004808"
     CDS             complement(<1..426)
                     /locus_tag="ACHOSM_RS24040"
                     /old_locus_tag="pgaptmp_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4561135.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVNRPGFPGEYFICELRLPDHYFRWKHNKLFLLLPEEYGP"
     gene            complement(690..1568)
                     /locus_tag="ACHOSM_RS24045"
                     /old_locus_tag="pgaptmp_004809"
     CDS             complement(690..1568)
                     /locus_tag="ACHOSM_RS24045"
                     /old_locus_tag="pgaptmp_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HGU4561136.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(1565..3793)
                     /gene="crfC"
                     /locus_tag="ACHOSM_RS24050"
                     /old_locus_tag="pgaptmp_004810"
     CDS             complement(1565..3793)
                     /gene="crfC"
                     /locus_tag="ACHOSM_RS24050"
                     /old_locus_tag="pgaptmp_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HGU4561137.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERYAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT          989 a         1050 c          940 g          923 t
ORIGIN      
        1 tgggccatat tcctccggca gaagcagaaa aagcttatta tgcttccatc ggaaataatg
       61 atctggcagc ctgagttcac agataaaata ctctccagga aacccggggc ggttcacaat
      121 agtgatagcg aggatcttct ggcgaccata tttatcgccc aacataccaa agaagagccc
      181 gccaagcggt cgaatcagaa agggaacgga gaaagtggca agtgcagcaa ccatctgcac
      241 gctggggtca gcccccggga aaaaaacttt acctaatgcg taagcaacaa aaccataaac
      301 accaaaatcg aaccattcca ttgcattacc cagtgatgct gcggtaatgg ctttacgcag
      361 tttaccgtca tcaataatgg tgacatcacg aagggtaatc ggttttactt ttttcctttt
      421 cagcatagct ttcctcgcag agttggccca tttccgcaaa cgccagaaaa atcagcgcga
      481 cggccgcaaa aggggagcgg gaattgcagg atgcaatctc tgtaacaagc gtagcgggtt
      541 tgcttacacc ctccggttaa ggaatgtaca atcaaaccag ttgacgcctg aattgggcag
      601 ttaatgacct ttttacccta ccagtgttta aatttgtgat caacttcaca catggatttc
      661 gtttaggact cattgatgta actgattatt tacgccaaat ttacagagaa gatttcctcg
      721 accattgccc ccaccaaacg ctgcgctgaa cagagtcgtg gcatccccag cgcgacagtt
      781 tgccaacgtt gggtcactga ccagcaaacg ggttgtttat ctgccgtggt ccactttccc
      841 agccaactta aaaaatcagc atgatccccg cccgtcggaa cggtcgaaga ggcaaaccac
      901 tgatggtaat agtgacgcgt ggtcggggcc gcattgatac tctctgccag atgttgtgtc
      961 gctttcacgc gcaaatcacc cagcagcaac gccaccgctt gcgggcttcc cagacgacag
     1021 gcatcgccaa atgcccccca acgcaactct tcgagaacga taaaacagcg cccaggcaac
     1081 gaccaggcgt tatacgcccc agcacgccag cgggtaaaaa tttgctcgct ctgatcacct
     1141 gcctgcacca gtaggcctcg ttgcgttaac gccttttcct tccagcgaca acgggctgaa
     1201 aaccactcat acaaggcggc aatttgctgg gggagctccg ggaaagcaga gtgctggcgg
     1261 tgatccattt tttgcagcag cgccagatgc tgcgcgatct gcgtcagcgc cagatgaccg
     1321 ggatccagca tcgacgccag tttttccacc attgtcagtc gcaggctaag cggcgcattt
     1381 aacatgccgc cgttcgtcca tgtctgaagc tgcacacgcg aaagaagttc ctgcatcagt
     1441 cgttcacgaa actgctgctg atgggcttgt ggaaggcggg catcatcgcc ctgcgccaga
     1501 tccactaaaa agcggggata aaccgactcc agcacgcgac cggggccgtc cagtaacgtc
     1561 ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc atcgcgtaac agctgggcgt
     1621 cggtgtaaat ccagccgtga gtcatcacag tctgctgcaa ttgctgctgc atcagcctga
     1681 ccaccgattc attttgttga cgcagagcca ggctttcgcg taaacgcgtc tgtaattccg
     1741 tcaaacatga agcgaactca gcgaaaaaag tattcatgcc tgccgtaaca gattcatcga
     1801 cctgctctgc cagaacttta gccatttgtt ggcaataaag atcgacttct gcgcttaatg
     1861 ctcgttgcaa cacactgtaa tcaaccgttt ctgtcgggga tttctcattt ccccgtcccc
     1921 agtcgggctg attcaaccag cgcgaaaaag tctcacgcac aacgcctaaa cgcgtgctct
     1981 gctcgtccgt tgcctcctgg cgcgaaatga cttcactgaa cagctggcga gtgttgacgt
     2041 ggggaactac tccgtgaaaa acaggaaaat gaaacccagg acgaaaccct gactcgctca
     2101 attccatttt gacttgttgc tcaatgggcc gaataacatc ggttaacacc cggcaaaggg
     2161 tggattcaag ctcggcaaaa cgcagcgtaa agtcgcgact gatggtgttc tgcgccgtct
     2221 gtaacagtgt ctcacagcag gtacgaatct cgcttaacgg ctccgaatca tcctgaaaca
     2281 aggcggctaa ctgcgcattc agcgcatctt gctgttgacg cagaaagtgg ttggcggagg
     2341 tcagggccag ctcgatttca tgtttaatct cgccgctcac ctgcgcctga ttgagttgca
     2401 atagctgcaa actttcttcg acctgatgga tattttgccg caattgttca caagcgacgt
     2461 ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg ctgcgcgtaa ttcaacagtt
     2521 tatgcgcagc agatcgcaga gcatacaatg aggcgttagc gtaagcggca tgaagcaacg
     2581 cctgaattgg ctgggcgaac agcgaatctt cccacaactg atcggcagca tgacgaatat
     2641 gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc gagcgcggca tgggcaaaat
     2701 cttccaccca gcgttgttgc tctggcgctg gtaacttacc gttgttggct aactcatagc
     2761 gcgcccgatt cgccaggtag ccccacatcg acgacaccgg aaatatctgc tgtggcgtaa
     2821 tacagccttt catcagcgtc ccggaaatca gtgcccgcac ctggtcggcg tcgtcactgt
     2881 tacgatcctg ttgatcgaac ttattgacca gcacatacag cggcaccgat tgccccaccg
     2941 ccaaaatcgc ctcacggacc tcttcatcgg agatcgattt cagttgcgta taatccagca
     3001 ccgccagtac cgccgaggcg cgtgccagct gctggttaag cattttttgc agatgcggtt
     3061 gcccggcttc atttggcccg ggggtatcca gtaacgtcaa ctgaccggga taactttcca
     3121 gccccgccag atggacaaac tccacttcaa tcacgggaat atgctcaatg gcggcgtaag
     3181 cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg cactaaatca ttcaaacttt
     3241 tcagacaatg aaaaataggc tgggcaccca gataatattt ttcgaaagcg acgccatttt
     3301 cgatccgctg cataagcgca cgcatatctt tatctatttc cagcacatcg gtcagatgct
     3361 taatatcgca atcacgcagg cgctgttgta attgttgaat taaacaatcg attggcgcga
     3421 catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt atggcgaata agcgtcggca
     3481 gcgcagtcat tgggcgatta cgattaggca gaacctccgt accaacaatg gcattaatgg
     3541 tggttgattt ccctgctttc atagtaccga caatcgccag caccatttcc agtcgggaaa
     3601 ttttacgcaa ctcattattc agcatcgcgt aacgttcggc gatattaggc tgactccagg
     3661 gtaaagccag ttgtggcgca tcgtctccgg gtacagagag aggcattttt tccagtaact
     3721 gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc ctgacttaac tcatacaggg
     3781 tctgtgtgta catagaaaat tcttccttaa agcaaatttt gttactttta tttagccaga
     3841 ttgtttttga gttctgtttt cggcttttat aattgctgca agaaataatt ttatatttag
     3901 tg
//
LOCUS       DAZKHC020000221         3822 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZKHC020000221 DAZKHC020000000
VERSION     DAZKHC020000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.222"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            60..761
                     /locus_tag="ACHOSM_RS24055"
                     /old_locus_tag="pgaptmp_004811"
     CDS             60..761
                     /locus_tag="ACHOSM_RS24055"
                     /old_locus_tag="pgaptmp_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4561138.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            786..3386
                     /locus_tag="ACHOSM_RS24060"
                     /old_locus_tag="pgaptmp_004812"
     CDS             786..3386
                     /locus_tag="ACHOSM_RS24060"
                     /old_locus_tag="pgaptmp_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGU4561139.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            3377..>3822
                     /locus_tag="ACHOSM_RS24065"
                     /old_locus_tag="pgaptmp_004813"
     CDS             3377..>3822
                     /locus_tag="ACHOSM_RS24065"
                     /old_locus_tag="pgaptmp_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4561140.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPG"
BASE COUNT         1061 a          833 c          958 g          970 t
ORIGIN      
        1 aattagtacc cctctagtgt tctggagggg atattcatat tttttaagag tgactattta
       61 tgaaaacttg cataacaaag ggaattgtga ccgtaagttt aacggcaata ttactcagct
      121 gctcatccgc atgggcggca ggtaaaggcg ggattggact tgcagcaaca cgtcttgttt
      181 attcagaagg tgaagagcaa atttcactgg gtgtacgtaa caccagtccg gatgttcctt
      241 atcttattca gtcatgggtg atgaccccag ataataaaaa atcagcagat tttattatta
      301 caccaccgct atttgtgctg aatccggcta atgagaatct gttacgcatt atgtacattg
      361 gtgcgccgtt ggcgaaagac agagaaaccc ttttcttcac tagcgtacgg gcagtccctt
      421 caacaacgaa gcgggaagag ggaaataccc tgaagattgc cacacaaagc gtcatcaaac
      481 ttttctggcg accaaaaggt ttagcgtatc ccttaggcga ggctccggcg aaactgcgtt
      541 gcacttcgtc agctgacatg gttacggtca gtaacccaac accttatttc attaccctga
      601 cagacctgaa aataggtgga aaagtagtta aaaatcaaat gatttccccc tttgataaat
      661 accaattttc tctgccaaag ggggccaaaa atagcagcgt aacgtatcga accatcaatg
      721 actacggggc ggaaacgccg caactcaact gtaaatcgta agccgtcttc agttaagaga
      781 gcgagatgta tagaactcat cgacaacaca gcctgttaag ctctggtgga gtgccatcgt
      841 ttattggtgg gctggtggtg tttgtgtcgg cagcgttcaa tgcacaagct gaaacctggt
      901 tcgatcctgc ttttttcaaa gatgatccct caatggtggc cgatttgtct cgtttcgaaa
      961 aaggacaaaa aataacgcca ggggtttatc gtgtcgatat tgttctgaat cagacaattg
     1021 tagatacgcg caacgtcaat tttgttgaga taacgccaga gaaggggatt gccgcctgtt
     1081 tgacgactga aagcctggat gcaatgggtg ttaatactga tgcgtttccg gcttttaaac
     1141 aactggacaa acaagcgtgt gtgccattgg cggagattat tccggatgcc agcgtaacgt
     1201 ttaatgtgaa taaactccgt ctggaaattt cagtaccgca aatcgccatc aaaagtaatg
     1261 cccgtggtta tgtcccccct gaacgttggg atgaagggat caacgcgcta ttactgggat
     1321 attcgtttag cggggctaac agtattcata gcagcgcaga cagtgattct ggcgacagct
     1381 attttctgaa tttaaacagt ggcgttaatt taggcccatg gagattgcgc aacaattcaa
     1441 catggagtcg cagtagtggc caaaccgcag aatggaagaa cctcagcagc tatttgcagc
     1501 gggcggttat tccactaaaa ggcgaactga ccgtaggtga tgattatact gcaggcgatt
     1561 ttttcgatag tgtcagcttt cgtggtgtgc agctggcgtc agatgacaac atgctgccag
     1621 acagcctgaa agggtttgcg cctgtggtgc gtggtatcgc caaaagcaat gcccagataa
     1681 cgattaagca aaatggttac accatttacc aaacttatgt atcgcctggt gcttttgaaa
     1741 ttagtgatct ctattccacg tcgtcgagcg gtgatttgtt agttgaaatc aaagaagcgg
     1801 acggcagcgt caatagctac agcgtaccgt tttccagcgt gccattactc cagcgtcagg
     1861 ggcgaatcaa atacgcggtg acactggcga aatacagaac caatagtaat gaacagcagg
     1921 agagcaaatt tgcccaggcc acgttgcagt ggggcggacc gtggggaacg acatggtatg
     1981 gtggtggaca atatgctgaa tattaccgtg ccgccatgtt tggtctggga tttaaccttg
     2041 gcgatttcgg agcaatttcg ttcgatgcga cccaggcgaa gagtacgctg gcagaccaaa
     2101 gcgaacataa aggtcagtca tatcgttttc tgtatgccaa aacgctcaac caattgggca
     2161 ctaattttca attgatgggc tatcgctatt cgacgtcggg tttctacacc ctttccgaca
     2221 ccatgtataa acatatggat ggctacgaat ttaatgacgg tgatgatgaa gatacgccga
     2281 tgtggtcgcg ttattacaat ttgttttaca ccaaacgtgg caaactgcag gtcaatatct
     2341 cccagcaatt aggcgagtac ggttcgtttt atttaagtgg tagccagcaa acttactggc
     2401 ataccgatca acaggatcgg ctattacagt ttggctacaa cacgcaaatt aaagatctct
     2461 cgctgggggt ttcctggaac tacagtaagt cccgtggtca acctgatgct gatcaggtgt
     2521 ttgcactaaa tttttccctg ccgctcaatc tgttgctccc cagaagtaat gatagctata
     2581 ccaggaaaaa aaattacgcc tggatgacct ctaacaccag tatcgataac gaagggcaca
     2641 ttacacaaaa cctgggttta acggagacac tactcgatga cggtaacctg agctacagcg
     2701 tgcaacaggg atataacagc gaggggaaaa cggctaatgg tagcgccagc atggactaca
     2761 aaggggcgtt tgcagatgcc cgagtgggct acaactacag cgataacggc agtcaacaac
     2821 aactgaacta cgctctttca ggcagtttag ttgcccattc acagggcatt accctggggc
     2881 aatcgctggg ggaaactaac gtgctgattg cagcaccagg cgcagagaat actcgtgtgg
     2941 cgaacagcac cgggctgaaa actgactggc gcggatatac cgttgtcccc tatgccactt
     3001 cttatcggga aaatcgaatc gcacttgatg cggcatcgtt aaaacgtaac gtggatcttg
     3061 aaaatgcagt agtcaacgtg gttcccacca aaggggcgtt ggttctggcg gagttcaatg
     3121 cccatgcggg agcaagggta ttaatgaaaa catcaaagca gggtataccg ctgcgttttg
     3181 gcgcgatagc gacgctggac ggcatacaga ctaatagcgg cattattgat gatgatggct
     3241 cgctctatat gtccggtttg cccgctcagg gagcgataac tgtacgctgg ggcgaagctc
     3301 ccgatcaaat ttgtcatatc agttaccagc ttaccgaaca acaaattaac tctgcgatta
     3361 cgcgaatgga tgccatatgc agataatctt tggagaaaaa tgcgtgttat tactacgact
     3421 attttttgcc gccgtcttaa tgctatggtg cgctcaaact gctgcttata gcgggcagtg
     3481 tcataccact caggggaatc cgtatattgg cgtcaatttt ggcgttaaaa ccctggagga
     3541 agaagaaaat acggctggag tagttaaaga caaattttat cagtggaacg aatcgaatga
     3601 ttattatgtt tcctgtgatt gcgataaaga caatgtcaga agtggccgat gggcattcgc
     3661 cgcggattca ccgttagtct atttaggcga caactggtac aaaattaatg actatcttgc
     3721 cgccaaagtt ttattgcagg ttaaaggcag ttctcctact gcggttcctt tcgaaaacgt
     3781 gggcacaggg gcagatacac gatggcatat ttgcgatccc gg
//
LOCUS       DAZKHC020000222         3783 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZKHC020000222 DAZKHC020000000
VERSION     DAZKHC020000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.223"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            167..361
                     /gene="ylcI"
                     /locus_tag="ACHOSM_RS24070"
                     /old_locus_tag="pgaptmp_004814"
     CDS             167..361
                     /gene="ylcI"
                     /locus_tag="ACHOSM_RS24070"
                     /old_locus_tag="pgaptmp_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4561141.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
     gene            complement(526..732)
                     /gene="ybcW"
                     /locus_tag="ACHOSM_RS24075"
                     /old_locus_tag="pgaptmp_004815"
     CDS             complement(526..732)
                     /gene="ybcW"
                     /locus_tag="ACHOSM_RS24075"
                     /old_locus_tag="pgaptmp_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HGU4561142.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            1018..1428
                     /gene="ybcV"
                     /locus_tag="ACHOSM_RS24080"
                     /old_locus_tag="pgaptmp_004816"
     CDS             1018..1428
                     /gene="ybcV"
                     /locus_tag="ACHOSM_RS24080"
                     /old_locus_tag="pgaptmp_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HGU4561143.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYAFDNSLLFTESIQNTTQIFPR"
     gene            1720..2013
                     /gene="bor"
                     /locus_tag="ACHOSM_RS24085"
                     /old_locus_tag="pgaptmp_004817"
     CDS             1720..2013
                     /gene="bor"
                     /locus_tag="ACHOSM_RS24085"
                     /old_locus_tag="pgaptmp_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HGU4561144.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(2104..2286)
                     /gene="rzoD"
                     /locus_tag="ACHOSM_RS24090"
                     /old_locus_tag="pgaptmp_004818"
     CDS             complement(2104..2286)
                     /gene="rzoD"
                     /locus_tag="ACHOSM_RS24090"
                     /old_locus_tag="pgaptmp_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HGU4561145.1"
                     /translation="MLKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(2503..2979)
                     /locus_tag="ACHOSM_RS24095"
                     /old_locus_tag="pgaptmp_004819"
     CDS             complement(2503..2979)
                     /locus_tag="ACHOSM_RS24095"
                     /old_locus_tag="pgaptmp_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGU4561146.1"
                     /translation="MQNLNPQRKAFLDMVAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            complement(2966..3271)
                     /locus_tag="ACHOSM_RS24100"
                     /old_locus_tag="pgaptmp_004820"
     CDS             complement(2966..3271)
                     /locus_tag="ACHOSM_RS24100"
                     /old_locus_tag="pgaptmp_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU4561147.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            complement(3593..>3783)
                     /locus_tag="ACHOSM_RS24105"
                     /old_locus_tag="pgaptmp_004821"
     CDS             complement(3593..>3783)
                     /locus_tag="ACHOSM_RS24105"
                     /old_locus_tag="pgaptmp_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGU4561148.1"
                     /translation="EYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTK
                     TRSKQKTTFSQQDIAKLD"
BASE COUNT         1111 a          889 c          762 g         1021 t
ORIGIN      
        1 aaatgataat aattatcatc tacatgtcac aacgtgcatc tacgccatca aaccacgtca
       61 aataatcaat tatgacgcag gtatcgtatt aattgatccg catcaactta acgtaaaaac
      121 aacttcagac aatacaaatc agcgacactg aatacggggc aacctcatgt caacgaagaa
      181 cagaacccgc agaacaacaa cccgcaacat ccgctttcct aaccaaatga ttgaacaaat
      241 taacatcgct cttgatcaaa aagggtccgg aaatttctca gcctgggtca ttgaagcctg
      301 ccgtcggagg ctaacgtcag aaaagagagc atatacatca attaaaagtg atgaagaatg
      361 aacatcccgc gttcttccct ccgaacagga cgatattgta aattcactta attacgaggg
      421 cattgcagta attgagttgc agttttacca ctttcctgac agtgacagac tgcgtgttgg
      481 ctctgtcaca ggttaagtag tttgaatgat tagcagttat ggtgatcagt caaccaccag
      541 ggaataatcc ttcatattat tatcgtgctt caccaacgct gcctcaattg ccctgaatgc
      601 ttccagagac accttatgtt ctatacatgc aattacaaca tcagggtaac tcatagaaat
      661 ggtgctatta agcatatttt ttacacgaat cagatccacg gagggatcat cagcagattg
      721 ttctttattc attttgtcgc tccatgcgct tgctcttcat ctagcggtta aaatattact
      781 tcaaatcttt ctgtatgaag atttgagcac gttggcctta catacatctg tcggttgtat
      841 ttccctccag aatgccagca ggaccgcact ttgttacgca accaatacta ttaattgaaa
      901 acattcctaa tatttgacat aaatcatcaa caaaacacaa agaggtcaga ccagattgaa
      961 acgataaaaa cgataatgca aactacgcgc cctcgtatca catggaaggt tttaccaatg
     1021 gctcaggttg ccatttttaa agaaatattc gatcaagtgc gaaaagattt aaactgtgaa
     1081 ttgttttatt ctgaactaaa acgtcacaat gtctcacatt atatttacta tctagccaca
     1141 gataatattc acattgtgtt agaaaacgat aacactgtgt taataaaagg acttaaaaag
     1201 gttgtaaatg ttaaattctc aagaaatacg catcttatag aaacgtccta tgataggttg
     1261 aaatcaagag aaatcacatt tcagcaatac agggaaaatc ttgctaaagc aggagttttc
     1321 cgatgggtta caaatatcca cgaacataaa agatattact atgcctttga taattcatta
     1381 ctatttactg agagcattca gaacactaca caaatctttc cacgctaaat cataacgtcc
     1441 ggtttcttcc gtgtcagcac cggggcgttg gcataatgca atacgtgtac gcgctaaacc
     1501 ctgtgtgcat cgttattaat tattcccgga cactcccgca gaggagtttc cctgtcaggg
     1561 ctgcggacat agttaatccg ggaatacagt gacgatcctt cgcatctgac atacattaat
     1621 aaatattaac aatatgagat ttcaactcat tgtttagggt ttgtttaatt ttctacacat
     1681 acgattctgc gaactttaaa aagcatcggg aataacacca tgaaaaaaat gctactcgct
     1741 actgcgctgg ccctgcttat tacaggatgt gcccaacaga cgtttactgt tcaaaacaaa
     1801 ccggcagcag taacaccaaa ggaaaccatc acccaccatt tcttcgtctc tggaattggg
     1861 cagaagaaaa ctgtcgatgc agccaaaatt tgtggcggcg cagaaaatgt tgttaaaaca
     1921 gaaacccagc aaacattcgt aaatggattg ctcggtttta ttactttagg catttatact
     1981 ccgctggaag cgcgtgtgta ttgctcacaa taattgcatg agttgcccat cgatatgggc
     2041 aactctatct gcactgctca ttaatatact tctgggttcc ttccagttgt ttttgcatag
     2101 tgatcagcct ctctctgagg gtgaaataat cccgttcagc ggtgtctgcc agtcgggggg
     2161 aggctgcatt atccacgccg gaggcggtgg tggcttcacg cactgactga cagactgctt
     2221 tgatgtgcaa ccgacgacga ccagcggcaa catcatcacg cagagcatca ttttcagctt
     2281 tagcatcagc taactccttc gtgtattttg catcgagcgc agcaacatca cgctgacgca
     2341 tctgcatgtc agtaattgcc gcgttcgcca gcttcagttc tctggcattt ttgtcgcgct
     2401 gggccttgta ggtaatggcg ttatcacggt aatgattaac agcccaagac aggcagacga
     2461 tgatgcaaat aaccagagcg gagataatcg cggttactct gctcatacct caatctctct
     2521 gaccgttccg ccagcttctt tgaattttgc aatcaggctg tcagccttat gctcgaactg
     2581 accataacca gcgcccggca gtgaagccca gatattgctg caacggtcaa ttgcctgacg
     2641 aatatcaccg cgatcaatca tcggtaaagc gccacgttct ttaatctgct gcaatgccac
     2701 tgcgtcctgg cttttggggg agaagtcttt caggccaagc tgcttacggt aagcatccca
     2761 ccagcgtgaa agaagctgat aacgtccggc tgctgttgat ttgagtttgg ggtttagcgt
     2821 gacaagtttg cgagggtgat cggagtaatc agtaaatagc tctccgccta caatgacgtc
     2881 ataaccatga tttctggttt tctgccgtcc gttatccgtt ccttctgacc atgccaccat
     2941 atcgaggaaa gctttacgct gaggattaag attttgcatt tttcacccct gtcagtcgtt
     3001 cccagaagta tgtcagtgca accgaaccca tcgcaccact aatccccgct gtcgcgagaa
     3061 tcatgtaaat actgaatcca ctttcgatac tgatcaggcc accaataaca ccggtgaatc
     3121 ccgacaccac tatctgagcc aaagcattta tccagctcca cgttgcttta ctctgcttca
     3181 catctatcag gtagcggacc agaccgcccc aacctgcgat gatcagcaaa acgagccaga
     3241 acgctccggc aaggctctct ttttcgtgca tatgaatagc caatgtttcg ccgccgacaa
     3301 aaggccggga cgttaaatgt cagaaatcag gctcacgggg taatttaacg acaaagcacg
     3361 gagttgatgc tccccgcaag cctggaataa aaaagccagc atgtagctgg caacagaggg
     3421 ttaagcaata tcaactcaac agctgaagac accctggctg gggtacgttg gaaggatact
     3481 caccgcccag aaacagaaaa gcccaaggct ttaaacctcg ggcttgaatt tggattacgg
     3541 ccagtgcgta caacattggc aaaatatcag atttacataa aatatatgat ttttaatcca
     3601 gttttgcaat atcttgctgt gaaaatgtgg tcttttgttt tgaacgtgtt ttcgttaaaa
     3661 gcaataaagc ttggctatca agctgtagaa aaatgtgctt cattgcaacc cagcgttcag
     3721 taaatgtctc agaccagttt tttgatgtca ctcccaccag tgatgccaac tcctggtatt
     3781 cat
//
LOCUS       DAZKHC020000223         3762 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZKHC020000223 DAZKHC020000000
VERSION     DAZKHC020000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.224"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            60..632
                     /locus_tag="ACHOSM_RS24110"
                     /old_locus_tag="pgaptmp_004822"
     CDS             60..632
                     /locus_tag="ACHOSM_RS24110"
                     /old_locus_tag="pgaptmp_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGU4561149.1"
                     /translation="MPGLISYVSSASFVNEMMELRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGRGRVYAELLNIAFNDGVNVLRNRILSALPGMGGRNSLQEKISECAF
                     TVDIEKLQCPGDALQCPITLEQPENGVFIKNSGNSAVCTLFDSVSFSHLVREGGVHPL
                     TRETITPSMIVKPEDCIFDSSKGNFIIKDG"
     gene            850..1548
                     /gene="nleD"
                     /locus_tag="ACHOSM_RS24115"
                     /old_locus_tag="pgaptmp_004823"
     CDS             850..1548
                     /gene="nleD"
                     /locus_tag="ACHOSM_RS24115"
                     /old_locus_tag="pgaptmp_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HGU4561150.1"
                     /translation="MRPTSLNLALHQSSTSSSMSDADIESLVKTSSVQWIKNNPQLRF
                     QGADHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVIAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRPESQKYSPLLLEEARTVGLGAFSEEVLSENKFREEIGMPRRTSYPHDSALIHDDNT
                     VSQGFLQVRLHPLL"
     gene            complement(1978..3125)
                     /locus_tag="ACHOSM_RS24120"
                     /old_locus_tag="pgaptmp_004824"
     CDS             complement(join(1978..2888,2888..3125))
                     /locus_tag="ACHOSM_RS24120"
                     /old_locus_tag="pgaptmp_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4561151.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNS
                     CCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIH
                     LDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KKVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSDQMSPTEYENQYYQRLGSV"
     gene            3188..3762
                     /locus_tag="ACHOSM_RS24125"
                     /old_locus_tag="pgaptmp_004825"
                     /pseudo
     CDS             3188..3762
                     /locus_tag="ACHOSM_RS24125"
                     /old_locus_tag="pgaptmp_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
BASE COUNT         1041 a          787 c          891 g         1043 t
ORIGIN      
        1 tggtgatgtt tgttaatgaa gacttgaggg aatatttatc cgtatgaagg aatatggtaa
       61 tgcctggatt aatatcatat gtgtcatcgg cttcattcgt gaatgagatg atggagctgc
      121 gtcagcaggt aatggagggg cagattggtg gatttctcct gggaggggag agggttagag
      181 tttcttatat gccagataca ggccgttttt tagcagaaag tgaagggcgg ggacgggttt
      241 atgcagaatt attgaatatt gcttttaatg atggagttaa tgtgctcaga aacaggatat
      301 taagtgcgct ccctggaatg ggagggcgaa actctttgca ggaaaaaata tcggagtgtg
      361 cctttactgt cgatattgaa aaacttcaat gtcctggtga tgcgcttcaa tgtccaatta
      421 cactggagca gcctgaaaat ggtgttttca ttaagaattc aggaaattct gcggtttgca
      481 ccctgtttga ttcggtttct ttttctcatt tggtccgtga aggtggggtt cacccactga
      541 cacgagagac aataactcca tcaatgattg taaagccaga agattgtata tttgacagtt
      601 caaagggaaa cttcattata aaagatggtt aaaatgtttt acatgaagca atattgattt
      661 tcggtagttt atataaaaca ccacagacat tcaaaatgtc agaggatgtc agtggtggat
      721 gtacctggag cggaagttgt ggaagtaagt tatgttatat cgcaataaac cgaatacatc
      781 tgtgatgtta cattggtctg agcttcagtg tgtggtgaat gaaactcgta acattttcag
      841 aggtccttga tgcgccctac gtccctcaac ttggcattac atcagtcatc aacgtcgagt
      901 tcaatgtcag atgcagatat cgagtctctt gtaaaaacat cgagcgttca atggataaaa
      961 aacaatccgc aacttcgttt ccagggggct gatcataata tatatcaaca gattgaagca
     1021 gcgctcgata agattggctc tacagagaca gggcgtgtac tcctgaatgc tattgaatca
     1081 atatcccgac ttaaatcaga aacagtggta atacacctca attcttccag actaggagtt
     1141 attgcacata gagatataga tgctgagaac catcggggaa ctggttccga ttttcactgt
     1201 aatctgaatg cagttgaata tccctgtggg gaggggatta gcgtggtgga ctttcatgcg
     1261 actattgttt ttcatgagct gctccatgtg ttccacaatt taaatgggga gcgcctgaaa
     1321 gttgagagtt cccgaccaga atcacaaaaa tactctccac ttttactcga agaagccaga
     1381 actgttgggt tgggggcttt ttcagaggag gtgctttcag aaaataaatt ccgcgaagag
     1441 attgggatgc cccgtagaac atcctaccct cacgactcag ctctcattca tgatgacaat
     1501 acagtgagtc agggattcct acaggtaaga ctgcatccat tgctttaggt gtgtttcttg
     1561 tggtgaatat atattggtgg gataaaactg agatacataa ggtctcacaa tggcttgcaa
     1621 ggctttgtgc ttctctgtag gggcgacatg tttttatccg gtgcgccgta aaaccccgtc
     1681 cttcagggcg tggaggatgt caaataataa ggaatagtgg atatgtttgc agcaaagccc
     1741 gagagcagtc aaagaagtat ttctggatta ttcagtccta ggtcaaatgg tgaaaacgca
     1801 agtaataaac cgccaagtgg tatggcgaac agaatcaaac cagttgtaag cgattcacaa
     1861 atcggtagat atacaactga cagtaatact ctgaaggatg gattgtgtag ccaagcccac
     1921 ataggaactc aaccttatcg gccaggtgct gactcgccgt gaatcgccac ggataatcta
     1981 gacacttccg agccgttgat aatactggtt ttcatattct gtcggtgaca tctgatcgct
     2041 ggaaccatgc cgacgcttac tgttataaaa catttcgatg taatcaaaaa tatcgctgcg
     2101 ggcttcttcc cgcgttccgt agatcttttt ctttatccgt tcacgtttca acaactggaa
     2161 aaaactttct gcaaccgcat tatcatggca gttaccgcga cggctcatgc taccctccag
     2221 gccgtgtgat ttcaggaacg actgccactc atggcttgtg tactgactgc cctgatccga
     2281 atgaaccagc accttttttt cgggattacg ccgccataca gccatcagca gtgcgttcag
     2341 gacaatgtcc tttgtcatcc gggattgcat ggaccagccg ataattttgc gtgagaacag
     2401 atcaacaaca acggcaagat acagccagcc ttcgtgggtc ctgatgtagg ttatgtccgt
     2461 tacccaacgc tcatcaggag catccggatt gaactgtcgc tggagcctgt tgggtgacac
     2521 gatactggcc tcgcctttac gtgcccgcgg gcttcggtat ccgacctgag cctttattcc
     2581 gacacgtttc atcagtctcc agactctgtt tactccgcac tgttgcccgc tgtcacgcag
     2641 atccagatgg attttgcgat aaccatagac gcatcccgat tccagccaga actgtttaat
     2701 ctgtcctgtc agtctcaggt ctgcctgatg gcgttgtgaa tgcggctgct gaagccaggc
     2761 gtaaaaacca ctgggatgaa catccagcac ccgacagagc aggcgaacag gccagcaaca
     2821 ggagttgtca cggataaagg cgtacctcag tcggacagct ttgcgaagta cgccgcggct
     2881 ttttttaata tgtcccgttc gtcggtaacc cgtttcagct ctttctggag acggcggatc
     2941 tcggcctgag catctgactg ttctttatta gtggaagaat ccggaccgta cttctttatc
     3001 caggcataaa ggctgtgggt ggtgatatcg agacgtgttg caacgctggc aacagaataa
     3061 ccgcgatcaa caacctgttt gactgcttca gttttaaact cttcgggata acgcttaccg
     3121 ctcatgggca cctctcttta agccatctta aatgactctg aggtgtctgt taaacccgtg
     3181 gcgattcaca acggggaaaa aaccgtatga tttattattc ctgaatgatg ctacaaaaca
     3241 agattttgat aaaaggaaaa tggagtttac atatcctgga gcaaataaaa gccatctaca
     3301 atcaagtaat agcgatgttg ttgctgctgc agctataagt attacagcga cagagatgaa
     3361 aaccatcctg ccagatgatt taacaccagg aaaatacaaa aaaatttatc tgtctgggga
     3421 tggttctgct ggtctaccac ttcttaagtg tggagatgaa tttttatcgc cgacagatat
     3481 tgtcgaccgc attgttcaac ataatcttca tgagattgat gatatcagat taacatcctg
     3541 taactcagcc aacatattaa aaaacaaaga cttctctcct gatgaaatag agaaatccgc
     3601 aaatatgaat aacggctggt tggctagggc attatttggt caaaagagtt ctttagcaga
     3661 acacgtctat gccgagtttg aacgtcgcgg aattaacgtt tctatatcag gttaccatgg
     3721 cactggcgtt ttttatgtac cagagcatgg taaaccaaca ac
//
LOCUS       DAZKHC020000224         3724 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZKHC020000224 DAZKHC020000000
VERSION     DAZKHC020000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.225"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            68..295
                     /locus_tag="ACHOSM_RS24130"
                     /old_locus_tag="pgaptmp_004826"
     CDS             68..295
                     /locus_tag="ACHOSM_RS24130"
                     /old_locus_tag="pgaptmp_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561152.1"
                     /translation="MACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(372..614)
                     /gene="yfdY"
                     /locus_tag="ACHOSM_RS24135"
                     /old_locus_tag="pgaptmp_004827"
     CDS             complement(372..614)
                     /gene="yfdY"
                     /locus_tag="ACHOSM_RS24135"
                     /old_locus_tag="pgaptmp_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HGU4561153.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            967..1887
                     /gene="lpxP"
                     /locus_tag="ACHOSM_RS24140"
                     /old_locus_tag="pgaptmp_004828"
     CDS             967..1887
                     /gene="lpxP"
                     /locus_tag="ACHOSM_RS24140"
                     /old_locus_tag="pgaptmp_004828"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HGU4561154.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2243..2314
                     /gene="ypdK"
                     /locus_tag="ACHOSM_RS24145"
                     /old_locus_tag="pgaptmp_004829"
     CDS             2243..2314
                     /gene="ypdK"
                     /locus_tag="ACHOSM_RS24145"
                     /old_locus_tag="pgaptmp_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HGU4561155.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2379..3617)
                     /gene="alaC"
                     /locus_tag="ACHOSM_RS24150"
                     /old_locus_tag="pgaptmp_004830"
     CDS             complement(2379..3617)
                     /gene="alaC"
                     /locus_tag="ACHOSM_RS24150"
                     /old_locus_tag="pgaptmp_004830"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HGU4561156.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRGRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          957 a          945 c          877 g          945 t
ORIGIN      
        1 attttcagga taactttcta tgaaagtaaa cttaatactt ttcagcttat ttttattggt
       61 ctctattatg gcatgcaatg tttttgcatt ttccatttct ggtggtgtaa gtgagaggag
      121 ctataaagag actgaaaaaa catcagcgat gacaaccaca cactctacaa aacttcagcc
      181 atcacaggcg attttgttta agatgagaga agatgcgcca ccattaaacc tcacagaaga
      241 aatcacgcct acttatccga caaaggcgaa ttatcttatt catcctgtgc gatagcgtct
      301 gtcacatggc aggtgaacaa tcatgtctga tgctgatgtg aacacctgcc caaatgagcc
      361 agcctgaccc actatcgcct gccatacccc accataaagc gaatcatcaa gccacaagcc
      421 ggggcaagaa acataaatat ccccgtcacc agttcattac cgccagttaa ccacatggtg
      481 aagtaataaa tgagtgcagc gaggatcacc atgccgaaga aagaagagac cgccgatacc
      541 acgttcagta cctgaccgat gtagccgctt acgcagacaa tacacagggc gagaaacatc
      601 cataaataaa tcatagctca acctcctgcc gcgctaaaaa acctgtaact tcagtcactt
      661 taatagtaag acaatcctta acctctggca atttttgcta agcatcaatg aaaacagcat
      721 gttaaatgca agactgttgt gtacggaaaa atatttactt tgcacgatta ataatcagta
      781 gctgaaagca gtcagcgagt gataaagtac agataccgca ttcatgctcc tcgtggttat
      841 gtcctgacta gtctttacac tctttacagg aaccattgtc gtacatgatg gcccaaccaa
      901 ttgagtgtac cgctgtcgac atggaatctt ctgcgataca acaattcgta tctacagaag
      961 gtaactatgt ttccacaatg caaattttcc cgcgagtttc tacatcctcg ctactggctc
     1021 acatggtttg ggcttggtgt actctggctt tgggtacagc ttccttatcc tgttctctgc
     1081 tttctcggca cgcgtattgg cgcaatggcg cgaccattcc tgaaacgtcg tgaatctatc
     1141 gcccgtaaaa acctggaact ttgtttcccg cagcattctg cggaagaacg cgagaagatg
     1201 attgccgaaa actttcgttc actcggcatg gcgctggtag aaaccggcat ggcatggttc
     1261 tggcccgaca gtcgcgtacg taaatggttt gatgttgaag ggttggataa ccttaaacgc
     1321 gcacaaatgc aaaatcgcgg cgtaatggtt gtcggcgtcc attttatgtc gctggaactg
     1381 ggcggccgcg tgatgggact gtgccaacca atgatggcta cctatcgtcc acataataat
     1441 cagctgatgg aatgggtgca gacccgtggg cgcatgcgct ctaacaaagc gatgatcggc
     1501 agaaataatc tgcgcggcat tgtcggtgca ctgaagaaag gtgaagcggt atggtttgct
     1561 cccgatcagg attatggtcg taaaggcagc tccttcgcgc cgttctttgc ggtggaaaat
     1621 gtcgccacaa ccaatggcac ctatgttctc tcccgtctct ctggcgcagc catgttgacc
     1681 gtaacgatgg taagaaaagc ggattacagc ggatatcgtt tgttcatcac cccagagatg
     1741 gaaggctacc cgacagatga aaatcaagcc gctgcctata tgaacaagat tatcgagaaa
     1801 gagatcatgc gcgcaccgga gcagtacctc tggatccacc gtcgctttaa aacgcgcccg
     1861 gtgggagaat cgtcgttgta catttaacaa actgctctta ctctttccct cctcaaatcg
     1921 gccataataa cctcatggtt tttatggctt ttttcttaag gttttttcct tcgctgaata
     1981 tctaattatt accgtcagaa attaaactgt cgccgacatg agacacttgt aagttactaa
     2041 atgtaaataa gaaaatgcct cttgtctcgt ttcagtttta ggcgtacatt agcgccgtct
     2101 cgagcagttt agctcagaat tctggggtga tgcccacggt aaaatacagg gcttttaagg
     2161 tcgctttgcc cggtgtcaac tcactgtatc aggtgtaatg aagtcattca ggcgtaacag
     2221 taattacgcg gagagatgta aagtgaaata tttctttatg ggcatttctt ttatggtcat
     2281 cgtttgggcc ggtacttttg ccctgatgat ctaaaagcaa actgtgcaat gaaaaacagg
     2341 agccatcagg ctcctgtttg catttctgac gacggtgttt attccgcgtt ttcgtgaata
     2401 tgtttgctgc tggcgggtag taaaccgtcg gcacggaaca tcgctttaat ccctctgatg
     2461 gcctgacgaa tacggccgcg gttttcaatc agtgcaaagc gaacatgcgt atcaccatag
     2521 tcgccaaagc caatccccgg cgagacacaa accttcgctt cgttaaggag cttcttggca
     2581 aattccagcg atcccatggc cgcatatggt tccgggattt tcgcccagac atacatcgaa
     2641 gccttcggca tttcgaccat ccagcccgct tcatgcagcc ctttaaccaa tacatcacgg
     2701 cggcgtttgt actgttcggc aatgtcgcgc acgcactgtt gatcgccctc aagtgccgca
     2761 atcgccgcca cctgcaacgg cgtaaaagtg ccgtaatcgt gatagctttt gattcgcgcc
     2821 agggcactga ccagcgtttt gttgccgacc ataaagccga tacgccagcc cgccatgttg
     2881 tagcttttcg acagcgtaaa gaactcgact gccacatcgc gtgcacccgg tacctgcata
     2941 attgacggcg ctttccagcc atcgtagacg atatcggcat aggccaggtc atggaccacc
     3001 agcacatcgt agcgtttcgc cagcgccact actttttcga agaactccag ttccacgcat
     3061 tgcgcggttg ggtttgacgg gaagccgaga atcatcatct tcggtttcgg ataactttca
     3121 cgaatggcgc gctccagttc gttgaagaaa tcgacacctt ccaccagcgg cactgagcgt
     3181 acctgcgccc cggcaatcac cgcgccataa atatggatcg ggtaacttgg attcggcacc
     3241 agcaccgtgt caccatgatc cagcgtcgcc agcatcagat gcgccaggcc ctctttcgaa
     3301 ccaatagtga cgatggcttc tgattccggg tcgatttcaa cgtcgtagcg atcctgatac
     3361 cagcgggaaa tggcgcgacg taagcgcgga atgccgcgtg acgtggagta accgtgcgtg
     3421 tccgggcgct gggccacagt acataatttt tcgacgatat gcggcggagt cgcaccgtcc
     3481 gggttaccca tactgaaatc gataatatct tcgccgcgcc gacgcgcagc cattttcagt
     3541 tcagcggtga tgttaaaaac gtagggcggg agacgatcaa tgcgcgtaaa gcgacgttca
     3601 gggcgagtgt cagccataat ttcctcagat taacgttagc gcccggaccg tccgagcgac
     3661 gctgccacaa tggtggctgt tttgaaaata gcctgattaa tttctgtctg tccagcgttt
     3721 taga
//
LOCUS       DAZKHC020000225         3683 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZKHC020000225 DAZKHC020000000
VERSION     DAZKHC020000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.226"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            50..628
                     /gene="espA"
                     /locus_tag="ACHOSM_RS24155"
                     /old_locus_tag="pgaptmp_004831"
     CDS             50..628
                     /gene="espA"
                     /locus_tag="ACHOSM_RS24155"
                     /old_locus_tag="pgaptmp_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HGU4561157.1"
                     /translation="MDTSTATSVASANASTSTSTVYDLGSMSKDQVVQLFNKVGVFQA
                     ALLMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIADVQSSSDKNKKAK
                     LPQEVIDYINDPRNDITVSGISNLNAELGAGDLQTVKAAISAKSNNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDIQSLQYRTISAISLGK"
     gene            641..1783
                     /locus_tag="ACHOSM_RS24160"
                     /old_locus_tag="pgaptmp_004832"
     CDS             641..1783
                     /locus_tag="ACHOSM_RS24160"
                     /old_locus_tag="pgaptmp_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     SctE"
                     /protein_id="HGU4561158.1"
                     /translation="MLNVNSDIQSMRSGASAATATSGINQPEVTSALDLQLVKSTAPS
                     ASWTESTALPTPPAGHSLVTPSVAEDVLSKLFGGISGEVRSRTEGTEPQRSTQNASSG
                     YPYLSQVNNVDPQAMMMMVTLLSLDASAQKVASMKNSNEIYTDGQNKALDNKTLEFKK
                     QLEEQQKAEEKAQKSKILGQVFGWLGVAVTAIAAIFNPALWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQVFGGVSIAASILTAGIGGVSSLMSKVGDVANKVGSNIVKTVTK
                     LADVFVENVVSKVAATANGFTTSARSIGTTVLNNDSAQYSILAQLSAYAVQNLTRQSE
                     NLGESAKVELDKAAAELRNQASYLQNASQLISDSARVNSRIVSGRV"
     gene            1804..2748
                     /gene="espB"
                     /locus_tag="ACHOSM_RS24165"
                     /old_locus_tag="pgaptmp_004833"
     CDS             1804..2748
                     /gene="espB"
                     /locus_tag="ACHOSM_RS24165"
                     /old_locus_tag="pgaptmp_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HGU4561159.1"
                     /translation="MNTIDYTNQVMTVNSVSENTTGSNAITASAINSSLLTDGKVDVS
                     KLMLEIQKLLGKMVRILQDYQQQQLSQSYQIQLAVFESQNKAIDEKKAAATAALVGGA
                     ISSVLGILGSFAAINSATKGASDIAQKTASTSSKAIDAASDTATKTLTKATESVADAV
                     EDASSVMQQAMTTATRAASRTSDVADDIADSAQRASQLAENAADAAQKASRASRFMAA
                     VDKITGSTPFIAVTSLAEGTKTLPTTVSESVKSNHEISEQRYKSVENFQQGNLDLYKQ
                     EVRRAQDDIASRLRDMTTAARDLTDLQNRMGQSVRLAG"
     gene            2755..3162
                     /gene="cesD2"
                     /locus_tag="ACHOSM_RS24170"
                     /old_locus_tag="pgaptmp_004834"
     CDS             2755..3162
                     /gene="cesD2"
                     /locus_tag="ACHOSM_RS24170"
                     /old_locus_tag="pgaptmp_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HGU4561160.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETSFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGTWFVMNVNS"
     gene            3199..3420
                     /gene="escF"
                     /locus_tag="ACHOSM_RS24175"
                     /old_locus_tag="pgaptmp_004835"
     CDS             3199..3420
                     /gene="escF"
                     /locus_tag="ACHOSM_RS24175"
                     /old_locus_tag="pgaptmp_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HGU4561161.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            3426..>3683
                     /locus_tag="ACHOSM_RS24180"
                     /old_locus_tag="pgaptmp_004836"
     CDS             3426..>3683
                     /locus_tag="ACHOSM_RS24180"
                     /old_locus_tag="pgaptmp_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscG/YscG/SsaH family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HGU4561162.1"
                     /translation="MVNDVSANKILVWAAVAAANHKLPKYAESILNVLPQIIPDKKDI
                     AHLEFIILYGLNRKNDAVKALEDFMDDETSQLLCSLVHENNR"
BASE COUNT         1096 a          721 c          865 g         1001 t
ORIGIN      
        1 ttttaatcgt ttttggtctt gctaagaaag attattaaga ggtatataca tggatacatc
       61 aactgcaaca tcagttgcta gtgcgaacgc gagtacttcg acatcgacag tctatgactt
      121 aggcagtatg tcgaaagacc aagtagttca gctatttaat aaagtcggtg tttttcaggc
      181 tgcgcttctc atgtttgcct atatgtatca ggcacaaagc gatctgtcga ttgcaaagtt
      241 tgctgatatg aatgaggcat ctaaggagtc aaccacagcc caaaaaatgg ctaatcttgt
      301 ggatgctaaa attgctgatg ttcagagtag ttctgacaag aataagaaag ccaaacttcc
      361 tcaagaagtg attgactata taaatgatcc tcgcaatgac attacagtaa gtggtattag
      421 caatctaaat gctgaattag gcgctggtga tttgcaaacg gtgaaggccg ctatttcggc
      481 caaatcgaat aacttgacca cggtagtgaa taatagccag cttgaaatac agcaaatgtc
      541 aaatacgtta aacctattaa cgagtgcacg ttctgatatt cagtcactgc aatacagaac
      601 tatttcagca atatcccttg gtaaataacc ggagataact atgcttaatg taaatagcga
      661 tatccagtct atgaggtctg gggccagcgc tgctacggct acttcaggta ttaatcagcc
      721 tgaagtgact tccgcgctgg atttacaact ggttaaatcc acggccccat cagcaagttg
      781 gacagaaagt accgctttac caacgccacc ggcaggccac tcattagtga cgccctctgt
      841 tgctgaggat gtcctgagta aactgtttgg tggtattagt ggtgaagtta ggagtcgaac
      901 agagggaacc gaaccacagc gcagcactca gaatgcaagc tctggctatc cttatctttc
      961 tcaggtgaat aacgttgacc ctcaggccat gatgatgatg gtcaccctgt tatccctgga
     1021 tgcttcagcg cagaaagttg cgagtatgaa aaactccaac gagatctaca cggatgggca
     1081 aaacaaggcg ctggataata aaacgctgga gtttaaaaag cagctcgaag aacaacagaa
     1141 agccgaagag aaagcacaaa aaagtaaaat tcttggtcag gtctttggtt ggttgggcgt
     1201 cgcggtaact gctattgcag ctatttttaa cccggcactc tgggctgttg ttgccgttag
     1261 cgcaacagca atggcactgc aaactgcagt tgatgtaatg ggcgataagg ccccgcaggc
     1321 gttaaagaca gcagcgcaag tctttggcgg agtatctatt gctgcaagta ttctgacagc
     1381 cggcattggc ggggtgtctt cacttatgtc taaagttggc gatgtcgcta acaaagttgg
     1441 ttcaaacatc gtgaaaacag ttactaaact ggccgatgta tttgttgaga acgttgtttc
     1501 aaaagttgcg gcaacggcaa atggatttac gacctctgca cgttctattg ggacaactgt
     1561 gctgaataat gattctgcgc aatacagcat attggctcag ttatctgctt acgcggtaca
     1621 aaacttaact cgacagagtg aaaacctggg tgagagtgcg aaggttgagc tggataaagc
     1681 tgcagccgag ttgcgaaacc aggcaagcta tttacaaaat gcttctcagt taatatccga
     1741 ctcagcacga gtaaatagcc gtattgttag tggtcgagtt taattattaa agagaattta
     1801 attatgaata ctattgatta tactaatcaa gtaatgacgg ttaattctgt ttcggagaat
     1861 actaccggct ctaatgcaat taccgcatct gctattaatt catctttgct taccgatggt
     1921 aaggtcgatg tttctaaact gatgctggaa attcaaaaac tcctgggcaa gatggtgcgt
     1981 atattgcagg attaccaaca gcaacagttg tcgcagagct atcagatcca actcgccgtt
     2041 tttgagagcc agaataaagc cattgatgaa aaaaaggccg ctgcaacagc cgctctggtt
     2101 ggtggggcta tttcatcagt attggggatc ttaggctctt ttgcagcaat taacagtgct
     2161 acgaaaggcg cgagtgatat tgctcaaaaa accgcctcta catcttctaa ggctattgat
     2221 gcggcttctg atactgcgac taaaacgttg actaaggcaa cggaaagcgt tgctgatgct
     2281 gttgaagatg catccagcgt gatgcagcaa gcgatgacta cagcaacgag agcggccagc
     2341 cgtacatccg acgttgctga tgacattgcc gattctgctc agagagcttc tcagctggct
     2401 gaaaacgctg cagatgccgc tcagaaggca agtcgggcaa gccgctttat ggctgcagta
     2461 gataagatta ctggctctac accatttatt gccgttacca gtcttgccga aggcacgaag
     2521 acattgccaa caacggtatc tgaatcagtc aaatctaacc atgagattag cgaacagcgt
     2581 tataagtctg tggagaactt ccagcagggt aatttggatc tgtataagca agaagttcgc
     2641 agagcgcagg atgatatcgc tagccgtctg cgtgatatga caacagccgc tcgcgatctc
     2701 actgatcttc agaatcgtat ggggcaatcg gttcgcttag ctgggtaatt gatcatggtc
     2761 gatacgttta atgatgaagt gcttaatcac tatcttgaac aaaaagggta cacaatacag
     2821 aaggagtttc tttgtggcag tgcctttttt atcggatggc ggattgagac ctcttttttc
     2881 tcattagcgt acagacttga tgaacaagaa ctgattttgt gctcttttga agcacgtaac
     2941 caaacagggc ttaacggccc tgttttatca ctgactcgct tgctcgaaga gttgtaccac
     3001 catttttcgg gtattaagaa aatcagtgcg atgaaatcta agattggttc agattcagaa
     3061 cgtcaaaagc gcgaagagtt gtttaattac ttcatcagaa agggcgctgt gcagcaagaa
     3121 acagaagacg gaacttggtt cgtaatgaat gtaaatagtt agaaattttt atttctatta
     3181 actattgagg aaaatttaat gaatttatct gaaattactc aacaaatggg tgaagtaggt
     3241 aaaacgctga gtgattctgt gccagagtta cttaatagca ccgatttggt taatgaccct
     3301 gaaaaaatgt tagagctgca gttcgcggtt cagcaatatt ctgcttatgt taacgtagaa
     3361 agtggaatgc tgaaaacgat aaaagatttg gtctcaacca tttccaaccg tagcttttaa
     3421 tttttatggt taatgatgtt tctgccaaca agatactggt gtgggcggct gtagctgcgg
     3481 caaatcataa gcttcccaaa tatgcagaat ctatcctgaa tgtattgccg caaattatac
     3541 ccgataaaaa agatatcgca catttagaat ttattatttt atatggatta aatagaaaaa
     3601 atgatgcggt aaaggctctg gaggacttta tggatgatga gacaagccag ttgttatgca
     3661 gtctggtcca cgagaataat aga
//
LOCUS       DAZKHC020000226         3674 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZKHC020000226 DAZKHC020000000
VERSION     DAZKHC020000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.227"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            23..241
                     /gene="ygeI"
                     /locus_tag="ACHOSM_RS24185"
                     /old_locus_tag="pgaptmp_004837"
     CDS             23..241
                     /gene="ygeI"
                     /locus_tag="ACHOSM_RS24185"
                     /old_locus_tag="pgaptmp_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HGU4561163.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIKAF
                     IVSTWMASFQNDMYSEDNPISPYYKIEW"
     gene            324..826
                     /locus_tag="ACHOSM_RS24190"
                     /old_locus_tag="pgaptmp_004838"
                     /pseudo
     CDS             324..826
                     /locus_tag="ACHOSM_RS24190"
                     /old_locus_tag="pgaptmp_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(845..1058)
                     /locus_tag="ACHOSM_RS24195"
                     /old_locus_tag="pgaptmp_004839"
                     /pseudo
     CDS             complement(845..1058)
                     /locus_tag="ACHOSM_RS24195"
                     /old_locus_tag="pgaptmp_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            1064..1288
                     /locus_tag="ACHOSM_RS24200"
                     /old_locus_tag="pgaptmp_004840"
                     /pseudo
     CDS             1064..1288
                     /locus_tag="ACHOSM_RS24200"
                     /old_locus_tag="pgaptmp_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
     gene            complement(1349..2469)
                     /locus_tag="ACHOSM_RS24205"
                     /old_locus_tag="pgaptmp_004841"
                     /pseudo
     CDS             complement(1349..2469)
                     /locus_tag="ACHOSM_RS24205"
                     /old_locus_tag="pgaptmp_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(2478..3245)
                     /gene="sctT"
                     /locus_tag="ACHOSM_RS24210"
                     /old_locus_tag="pgaptmp_004842"
     CDS             complement(2478..3245)
                     /gene="sctT"
                     /locus_tag="ACHOSM_RS24210"
                     /old_locus_tag="pgaptmp_004842"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HGU4561164.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLERYASQLNAFAISLTV
                     KSALAFLILIIYFGPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(3247..3506)
                     /locus_tag="ACHOSM_RS24215"
                     /old_locus_tag="pgaptmp_004843"
                     /pseudo
     CDS             complement(3247..3506)
                     /locus_tag="ACHOSM_RS24215"
                     /old_locus_tag="pgaptmp_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
     gene            complement(3516..>3674)
                     /locus_tag="ACHOSM_RS24220"
                     /old_locus_tag="pgaptmp_004844"
     CDS             complement(3516..>3674)
                     /locus_tag="ACHOSM_RS24220"
                     /old_locus_tag="pgaptmp_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU4561165.1"
                     /translation="VISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQ
                     YFDLSINP"
BASE COUNT         1268 a          701 c          608 g         1097 t
ORIGIN      
        1 gacaattaaa aaagggagct ttatgactaa tccaatcggt atcaataatt tatctcaaag
       61 ttcaaatata gcgaatgcaa cgggtgatga agtcgtatca ttagataaac acatcaacac
      121 cagtgctact gatacagatc aaataaaagc atttatagtt tcaacatgga tggcgtcttt
      181 tcaaaatgat atgtattcag aagataatcc tatctcacct tactataaaa ttgaatggta
      241 gacgattgac caaataaatc ataacgagat acttttataa tagtatctcg ttcctttcat
      301 tcaatcctca cacatgaaaa aatatgcact caccaaaagt taaattctta accatcttca
      361 cgttttgtat atttattaca aaaatgtctt tcgccagcaa ctcttgctct aatgaagcag
      421 gaacaatgtt cagaatcgaa ccaaatttaa ttaaagctat cgctcttgtt gaaagcaatc
      481 taaaaaagga tagcataggc aagaacagag ataagaataa caatataaag agtttagatt
      541 atgggttaat gcagataaac cagatgcata ttcccctgat aaaaaaacgt ggaataataa
      601 aagatgaacg agatttgtta gataaccctt gtctgaacat taaaatagga accgagattt
      661 tatataacca tttttcacgt tgtggagtaa cgtggcaatg cctggggact tataatgcgg
      721 gttttgcaat ggataaccaa aaaaagagac agcaatatgc ccaaaaatat atattgtata
      781 taccaggctt aatgaactag acaatcgtaa ggcttccgca aaatgagcct aaagcctctt
      841 ttttttatat agtgcaaaca cccatacgta aagctgtttt gatcaactcg ataccactgt
      901 gaacatctaa tttcttcatg atattgagtc tatgagtttc gactgttttc ctgcttaact
      961 gcaagaaatt agcgattact ttgtttgttt gttttaccat cagctatgag ttgtaatatt
     1021 tctgactcac gatttgtaag ttgattatca gaggaatacc agcaccagag aaaattcatt
     1081 aaaggagaat caaaataaaa gaagtggcga atcccaagcg atagtagatg caagacagtt
     1141 ctttagcgac cccccggaat atatatcatc agcagaactg gagcaaaagc tatatcgcga
     1201 atttgcacag gttactacag ttccagctta taaagagatg agtatgtatc aattgcttat
     1261 catttcacaa gatcggttaa ctcattaaat aaggatctac acgctttttg cgtgtagatc
     1321 cttataaatt tctgactacg acagatgttt aattttttaa attatcgtct ttattctcga
     1381 tttttgtgtc ttcaccctca atgaatttat cttctgaaga gagctgttca tcttgaacag
     1441 gttgcccagc attctccaca tcttcaagcc acagcagaag acgtaatatt tcatctatat
     1501 tttcgaagct aacataatcg tagcgacgat gggtagcata aatttttcgt gctaattttt
     1561 tatctgtgat aattggtatt ccgatttcct ttgcatattt ccttacgcct aaggccacct
     1621 cattagttac tcttacagaa atcaatggaa taggtgaaag atgtggctta aagtatatcc
     1681 ctattgcaat gtgagtaggg ttggaaataa tcaaacgact ataactgaca tcagacttca
     1741 attgctcaga aagaatttcc tgatgcgctc tctgcgttta gacttaattt ctggatttcc
     1801 ttcttgctcc ttgtattctc ttttaacttc ttgtttatcc attttcatat ctttcataaa
     1861 taaaaaatat tcagcgataa aatcaaaaat taagacaatt atcatactgc cgagacaata
     1921 cagtatgaga aggaatagca tctctcccca atctgataat aaagatatga tatctccatc
     1981 aagagtttta aaaagcagtg atttatgatt actccaaaat actttgatcg ccaatgcaaa
     2041 aaaaaacaat ataaagaatt gctttaacaa attcttttac ggtttttaag ccaaatatac
     2101 gctttaagcc atttactgga ttcaatgaat caaacttaaa tttaacagct tcagttgcta
     2161 attgcatttt actttgaaac caggagacaa gtattgttgt gaacatgcaa accagcaaaa
     2221 aagaaatgtt cgttttcaac caaccacccc catagcttta aaatagtccc aaatatctgg
     2281 tttagcgtta tgatcaagga tgtattcaag aatcgacata atgtgatgca catcaaaaac
     2341 atatccgaga tataaagtac ccacaagcat tattacagag atggttaagt ctctactttt
     2401 tagaatttgc ccttttttag aagcatcctg gagtttcttt tgtgttggct tttcagtttt
     2461 attggccatg aaacactcta tttctcaatg taaagttgca attgttctgg aaaaaaggat
     2521 aacggcatta ctcgttcagc cagtattggg ccaaaataaa taatcaatat taaaaatgcc
     2581 agcgcacttt tgacagtcag tgaaatggca aaagcattta gttgtgaagc ataacgttcc
     2641 agcaatccca ggacagcctc accgcccagc ataacggcaa tcacggggct tgcgtaaacg
     2701 atagtatgag tcattatgtt attaatatat gagaataaag gctctaattt aggaaaatta
     2761 aaattgcctg aaggccataa attataactt tgccaaagag tttccagaat aaagttcatt
     2821 ccacctttag taagataaac cgcagcagag aaaaggttaa ataatcttgc taattccgag
     2881 gtatcgacac ctgtagccgg atccaaagta ctacttaatg ttgcaccgcg ttggttatcg
     2941 ataaagctac ctatagcaag gaaaatccaa aacggggatg ctaataagca tgcaataaac
     3001 agccctatga taatttctct ggcagcgaac aaagcaatat cgatttcatt taagttcaat
     3061 aattcatagt ggtaatgctg aactaatgca catgatacca ctattattat tgggtgacga
     3121 actactgttg gtatattccc actggcgaaa aatggtaaca aatagaaagt tggtgccaat
     3181 cggcaaaaac cgagcgctgt agcagccagc agactatgca actgatagag tatcgcctcc
     3241 cccatgctat cctctcgcga atgctaaatt cagcatttcg ataccaaatg aatataattt
     3301 ttcaccatac cacccgctca ttaaaaaaaa gcaaatactt acgcacaata atttcacgcc
     3361 aaatggcaac gtctgttctt gcaactgcgt taccgtttga aaaagcccta ccaataaacc
     3421 aacaaatgtc gcaactgcaa taggtcctgc agacataact aaaatcagat acagcgccta
     3481 ttccccgcaa aaacaatatc gtccatattt tttcctcagg gattaatgga taggtcaaaa
     3541 tattgtaaaa tgagcccttt tgataacatt gtccaaccgt ccatcgcgac aaacaaaatt
     3601 aattttatcg gtgtagatat ggttaccgga ctcatcatca tcatacctaa cgttaacaga
     3661 acgctggata taac
//
LOCUS       DAZKHC020000227         3638 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZKHC020000227 DAZKHC020000000
VERSION     DAZKHC020000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.228"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(18..359)
                     /locus_tag="ACHOSM_RS24225"
                     /old_locus_tag="pgaptmp_004845"
     CDS             complement(18..359)
                     /locus_tag="ACHOSM_RS24225"
                     /old_locus_tag="pgaptmp_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4561166.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            complement(352..3594)
                     /locus_tag="ACHOSM_RS24230"
                     /old_locus_tag="pgaptmp_004846"
     CDS             complement(352..3594)
                     /locus_tag="ACHOSM_RS24230"
                     /old_locus_tag="pgaptmp_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4561167.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTAQGELKT
                     LTEMQKQELLRNAALIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERARE
                     RMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQEAFYAES
                     DVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRNW
                     ASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
BASE COUNT          710 a         1236 c          836 g          856 t
ORIGIN      
        1 ttcgtgaata tcctgcatca gttcaccacc tgctctatcg tgcaactgaa atcactgtac
       61 cgggcattat ccgtgacact ccactcacgg cacacaaccc tcaccgtccg gttatgtttc
      121 ggcggtcgcc acaaaaaggc acggtaacca ccatgccacg ataaaaactc ttccagccag
      181 cgccgggttg actcatccgt cacccggaac accgcctgaa acgtcttcag ttgaggattc
      241 agccctgtgg ggcggcgctg ttcataaccg tcaccaaacc gcaccctcac caccgacggc
      301 ttctcactca cctgcatccc ttcacgcggg accagatgca gcgtttttat ctcagccact
      361 cagcattcct ccgtcacgtc gcatggacag catcaccgcc tgcacccgct ggtcaatcag
      421 ctgcacaaga ctgcctgccg cctccggccc tatctggcca ttagtcccgt cattctgaat
      481 ggcgatatgg tagaccgggg aatacaccag acccgcacta ccgttcatac tacccaccgc
      541 gcgcacaccc agcgagccat ccgccgcccg ggtcaggggc ataatggctt caggaccggc
      601 ctcccccatc agccccgccc cttttgcaaa ggcaaagtac gtgggcgtat ccacaatact
      661 gttgctgtac gcactcaggt ttgccgaggt atacacgccg ccttttgcat tggccaccgc
      721 cccgcccagc cagtcaccaa tgctgccgag aaatcctccc gcaccggaca taccgtttgc
      781 cgccgtctta attccgttga caatggccgc attcataaga acttttgata tttcctgcag
      841 tacggatgag gcccagttgc gccattccac tttatttccg ttcagcatct ccgtgatgtt
      901 attcaccatc cctgagatac cctccgtcgc aagctgtgct gcctgtgagg cgtaatcgga
      961 cgcattatcc acccagttac tgaacccctc ctgcagccct ttctgccagt ccgcacgctg
     1021 cacatccgat tcggcataaa aggcttcctg ctctttcaga cgttcactca gatactgtgc
     1081 attctgcgcc agagcctgtc tgtaaaaatc ctcactgata tccccggtct gatactgaga
     1141 ctgaaggtcc gcatccttct ggcggaagct gtcgcggatc tgctgcaact cccgcatgcg
     1201 ttctctggct cgttccccct gcccgtaccc cagcagttca gcatcattcg acgcacgcgc
     1261 agccgcattc tcattcttca gtgtctcttc ccgggatcgc aactgttccc ggattttttg
     1321 ctggtcaatc agggccgcat tacgcagcag ctcctgcttc tgcatctccg tcagggtttt
     1381 cagttcgccc tgcgcagtct ggtatttcag cttcgccagc tctgtattct gccccaccag
     1441 tgccagttgc tctttctgct gcttcagcag ccgggaaaaa ctgtcttccg ctttttccgt
     1501 ctctgatttt ccaccccggg atttgggttt attcgcctcg ttattgcgcc aggcttccag
     1561 ggcattactg atataacgtt gtctcgcctc ctgatacgga tcacccacaa aaccgaggtc
     1621 atccgccgca taccccagcc ggacacgctc tttttcttcc cctttcagtc tggacagggc
     1681 cagctcacgc tctgtttttg tcagggcact ctgctgttta tcatccagag tggcctgtgg
     1741 cagccgtaac ggcacattca ccagtccctg ccgctgctga agcagttcat tacccagccc
     1801 cagcagacgg ttgaattccg tatgctgacc attcataacc agcatggact ggtacacctt
     1861 attctgctct gccgcctgct gacgaattaa cgccacacga cggtcttcca gcccggcaag
     1921 cacatcctga atggactgcg ctttttcctg catctgtgcc agacgggact gctcaacggc
     1981 aagctgctct gttgcctgag caagcccttc cgttacggtc ttcaccgagg tcagatggtt
     2041 tatcatgaat ccgtcaccgg tcgtccagcc cgggttcgcc agaacatact gatatcctgc
     2101 gattttttcc tgcagggatt tcacccgact ggcctgttca tcaatcagcc ggttctgctc
     2161 tgtcagcgcc gcccgtgttc gtccttcatt atctgaggct tcaggcaaag acattgacgg
     2221 cgttttatgc gcgatttcat ctatcgtcag tgcatactgg cgcgcagact ccctggcctg
     2281 ctcctgattc tggtacagcg tgtaccatgc tgcagccccc agcatcacca gtccgggtac
     2341 gccaccaacc agccccagcg caccgctcat cagacgtgag cccaccgccg ttgtactgtt
     2401 cagcgcattc tgggcggcgc ttctggcagc aatatttctg ttcaggcgtt cctgtgtggc
     2461 cgccagacgg gcctctgcag caatctgcat ctccgtcccg cgggctgccg ccacggcctg
     2521 ctgagcacgg tacacggctg cccttgcccg cgccgtggca atctgcgttc ccctgaactg
     2581 tgcttccgcc agtgcaactt cattacgtgc agccgtcaca agtcctgccg tggcagacat
     2641 cgctccggag gccatattgc caaagtaccg ggcaaccccg acggcaacca gcgcgcccac
     2701 ggctgttgcc acattatcaa tctgtccggc aacaccgttc agcatgccgg agagcgtttt
     2761 tgtcaccccg ctggcctcat tcgcaccgcc cacccaggcc ataaaggcgt tttccacctt
     2821 tgtgatacta ctggaaaccg tttccggcat ggccgcatat tcatcacgta atatccccag
     2881 ctggctgatt aacgcgggga ccactttatc cgctgtcagt tttccgtcat ccgccattgc
     2941 cttcagatct ttacgggcca cgcccatacc cgcagccagt gcacgtacga tccggtcacc
     3001 actttcattg accgaattaa actcctcacc acgcaataca ccctgcgcca gcgcctgact
     3061 gaactgggtg atcaccgagc ccgcctctgc cgtactggca ccggagattt tcagccctgt
     3121 cgaaatggcc tccgtcacct tcagcacatc atcagcactg taaccatatt cacgcatcga
     3181 ggcagccgaa cgggcaaaca gggccgcatt atccgaaaat gcggtgcctg tccgctggct
     3241 gatatccatc agcactttct gtgatgacgc aaattcatcc gatgactgcg acgcctgttt
     3301 cagacgggca ttcacggagc tccattcatc cgccagcgaa atcaggtgtc cggtggcaaa
     3361 ggcaccggca aacgcaccgg tcattccgac agccgaagcg cggatttccg tcaactggct
     3421 gtgcagctca gccagagccc ggcgctgctc cctggctgcc gcagcagcct gacgtccgcc
     3481 attctgcagg gtccggtaat attcactgcc catacgggac gcccgctgga tctccgactg
     3541 gaatgactgt gaatttgccg aaattttgat aatcagttca cgtaacgtcg ccattcacct
     3601 ttctccgggc aaaaaaaacc tgccacagca ggttttca
//
LOCUS       DAZKHC020000228         3603 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZKHC020000228 DAZKHC020000000
VERSION     DAZKHC020000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.229"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..73
                     /locus_tag="ACHOSM_RS24235"
                     /old_locus_tag="pgaptmp_004847"
     CDS             <1..73
                     /locus_tag="ACHOSM_RS24235"
                     /old_locus_tag="pgaptmp_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561168.1"
                     /translation="LLQMTGEWLNTTGAAQQSPEHRS"
     gene            313..567
                     /locus_tag="ACHOSM_RS24240"
                     /old_locus_tag="pgaptmp_004848"
     CDS             313..567
                     /locus_tag="ACHOSM_RS24240"
                     /old_locus_tag="pgaptmp_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HGU4561169.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCM"
     gene            803..877
                     /gene="tap"
                     /locus_tag="ACHOSM_RS24245"
                     /old_locus_tag="pgaptmp_004849"
     CDS             803..877
                     /gene="tap"
                     /locus_tag="ACHOSM_RS24245"
                     /old_locus_tag="pgaptmp_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HGU4561170.1"
                     /translation="MLGKVQDFFLCSLLLRIVSAGWCD"
     gene            870..1727
                     /gene="repA"
                     /locus_tag="ACHOSM_RS24250"
                     /old_locus_tag="pgaptmp_004850"
     CDS             870..1727
                     /gene="repA"
                     /locus_tag="ACHOSM_RS24250"
                     /old_locus_tag="pgaptmp_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HGU4561171.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRASRALTFLAELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVVAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANGEAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2639..2923
                     /locus_tag="ACHOSM_RS24255"
                     /old_locus_tag="pgaptmp_004851"
     CDS             2639..2923
                     /locus_tag="ACHOSM_RS24255"
                     /old_locus_tag="pgaptmp_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HGU4561172.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            2923..3198
                     /locus_tag="ACHOSM_RS24260"
                     /old_locus_tag="pgaptmp_004852"
     CDS             2923..3198
                     /locus_tag="ACHOSM_RS24260"
                     /old_locus_tag="pgaptmp_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4561173.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(3328..>3603)
                     /locus_tag="ACHOSM_RS24265"
                     /old_locus_tag="pgaptmp_004853"
     CDS             complement(3328..>3603)
                     /locus_tag="ACHOSM_RS24265"
                     /old_locus_tag="pgaptmp_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4561174.1"
                     /translation="TERLKEAKLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKN
                     RAEVELATLTWVDWYNNRRLLGRLGHIPPAEAEKAYYASIRNDDLAA"
BASE COUNT          914 a          928 c          899 g          862 t
ORIGIN      
        1 actgctgcag atgaccggag aatggctgaa tacaacaggg gctgctcagc agtcaccgga
       61 acatcgtagc tgacgccttc gcgttgctca gttgtccaac cccggaaacg ggaaaaagca
      121 agttttcccc gctcccggcg tttccataac tgaaaaccat actatttcac agtttaaatc
      181 acattaaacg acagtaatcc ccgttgattt gtgcgccaac acagatcttc gtcacaattc
      241 tcaagtcgct gatttcaaaa aactgtagta tcctctgcga aacgatccct gtttaagtat
      301 tgaggaggcg agatgtcgca gacagaaaat gcagtgactt cctcatctgg cgcaaaacga
      361 gcatacagaa aggggaatcc gctttctgat gcagagaaac aaagattatc agtggcccgt
      421 aaaagagctt cgttcaagga agtaaaagta tttcttgaac caaagtataa ggccatgctc
      481 atgcaaatgt gtcatgaaga tggcctgact caggctgaag ttctgaccgc actgataaaa
      541 agtgaagcgc aaaaacgatg tatgtgatga tgggcttaca ttcttgagtg ttcagaagat
      601 tagtgctaga ttactgatcg tttaaggaat tttgtggctg gccacgccgt aaggtggcag
      661 ggaactggtt ctgatgtgga tttacaggag ccagaaaagc aaaaaccccg ataatcttca
      721 gaaagtttgg cgactgagaa gattaccggg acccacttaa accgtatagc caacaattca
      781 gctatgcggg gagtatagtt atatgctcgg aaaagttcaa gacttctttc tgtgctcgct
      841 ccttctgcgc attgtaagtg caggatggtg tgactgatct tcaacaaacg tattaccgcc
      901 aggtaaagaa cccgaatccg gtgttcacac cccgtgaagg tgccggaacg ctgaagttct
      961 gcgaaaaact gatggaaaag gcggtgggct tcacctcccg ttttgatttc gccattcatg
     1021 tggcacatgc ccgttcgaag ggactgcgtc ggcgcatgcc accggtactg cgtcgccggg
     1081 ctattgatgc gctgctgcag gggctgtgtt ttcactatga cccgctggcc aaccgcgtcc
     1141 agtgctccat cactacgctg gccattgagt gcggactggc gacggagtct gctgccggaa
     1201 aactctccat cacccgggcc tcccgagcct tgacgttcct tgcagagctg ggactgatta
     1261 cctaccagac ggaatatgat ccgcttatcg ggtgctacat tccgaccgat atcacgttca
     1321 caccggcgtt atttgccgcc cttgatgtgt ctgaggatgc agtggttgct gcgcgccgca
     1381 gtcgtgttga atgggaaaac agacagcgca aaaagcaggg gctggatacc ctgggtatgg
     1441 atgaactgat agcgaaagcc tggcgttttg tgcgtgagcg tttccgcagt taccagacag
     1501 agcttaagtc ccgtggaata aagcgtgccc gtgcgcgtcg tgatgcgaac agggaacgtc
     1561 aggatatcgt caccctggtg aaacggcagc tgacgcgtga aatctcggaa gggcgcttca
     1621 ctgctaatgg tgaggcggta aaacgcgaag tggagcgtcg tgtgaaggag cgcatgattc
     1681 tgtcacgtaa ccgtaattac agccggctgg ccacagcttc tccctgaaag tgacctcctc
     1741 agaataatcc ggttcgcgcc ggaggcatcc gcacacctga agcccggcgg cgcacaaaaa
     1801 atcagcacca catacaaaaa aacaacctca ccatccacct tctggtgcat ccggtccccc
     1861 ctgtttttga tacaaaacac gcctcacaga cggggaattt tgcttatcca cattaaactg
     1921 caagggactt ccccataagg ttacaaccgt tcatgtcata aagcgccagc cgccagtctt
     1981 acagggtgca atgtatcttt taaacacctg tttatatctc ctttaaacta cttaattaca
     2041 ttcatttaaa aagaaaactt attcagtgcc tgtcctgtgg acagacagat atgcacctcc
     2101 caccgcaagc ggcgggcccc gaccggagcc actttagtta caacactcag atacaaacac
     2161 cagaaaaacc ccggtcccgc gcagaactga aaccacaaag cccccctcat aaccgaaaag
     2221 cggccccgcc ccggcccttc gggccggaac agagtcgctt ttaattatga atgttgtaac
     2281 taagcatcat catcgctgtc agtcttctgg ctgaacgttc tcagtacacg ctcgtaagcg
     2341 gccctgaggg cccgctaacg cggagatacg ccccgacttc gggtaaaccc tcgtcgggac
     2401 cactccgacc gcgcacataa gctctctcat ggctgaaagc aggtatggtc tggcagggct
     2461 gggggggggt aaggtgaaat ctatcaatca gtaccggctt acgccgggct tcggcggttt
     2521 tacacctgtg ctacatgtaa cctccgtata ccgccttaca tgcgctgacg cgcattattt
     2581 gccttgttcc gtctgaaagt aatcactatg attaaatatg attaacgact aatcggatat
     2641 gcaaatgaaa aacaataccg cacaagcaac aaaagtcatt accgcgcatg tgccattacc
     2701 tatggctgat aaagtcgacc agatggccgc cagactggaa cgctcccggg gctgggttat
     2761 caaacaggcg ctttctgcat ggcttgccca ggaggaggag cgtaatcgcc tgacgctgga
     2821 agccctggac gatgtgacat ccggacaggt tattgaccat caggctgtac aggcctgggc
     2881 ggacagcctc agtactgaca atccgttacc ggtgccacgc tgatggaact gaagtggacc
     2941 agtaaggcgc tttctgattt ggcgcggtta tatgattttc tggtgctagc cagtaaacct
     3001 gctgccgcca gaacggtaca gtccctgaca caggcaccgg tcattctgtt aactcatcca
     3061 cgtatggggg aacagttatt tcagtttgaa cccagggagg tcagacggat ttttgctggc
     3121 gagtacgaaa tccgttacga acttaatggc cagactattt atgtattgcg cctgtggcac
     3181 acacgagaaa acaggtagat tgtgtgtggg gacaacttaa catagcctgc agatattaat
     3241 tctggaacat tattatcagg ccagatattt aaataatctt gtaggtgaac cgccccggtt
     3301 ttcctggaga gtgttttatc tgtgaactca ggctgccaga tcatcgtttc tgatggaggc
     3361 ataataagct ttttctgctt ctgccggagg gatatgaccc agccttccca gcaatcgtcg
     3421 attgttatac cagtccaccc acgttagtgt ggccagttcc acttctgcac ggtttttcca
     3481 gctcttacgg tgtattacct ccgctttgta aagaccattg atgctctccg ccatcgcgtt
     3541 gtcatatgag tcacctgtac tccctgttga tgccagcagt tttgcttctt ttagtcgctc
     3601 cgt
//
LOCUS       DAZKHC020000229         3528 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZKHC020000229 DAZKHC020000000
VERSION     DAZKHC020000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.230"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            84..2174
                     /gene="iha"
                     /locus_tag="ACHOSM_RS24270"
                     /old_locus_tag="pgaptmp_004854"
     CDS             84..2174
                     /gene="iha"
                     /locus_tag="ACHOSM_RS24270"
                     /old_locus_tag="pgaptmp_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HGU4561175.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPFPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKVGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(2392..2542)
                     /locus_tag="ACHOSM_RS24275"
                     /old_locus_tag="pgaptmp_004855"
                     /pseudo
     CDS             complement(2392..2542)
                     /locus_tag="ACHOSM_RS24275"
                     /old_locus_tag="pgaptmp_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(3107..3528)
                     /locus_tag="ACHOSM_RS24280"
                     /old_locus_tag="pgaptmp_004856"
                     /pseudo
     CDS             complement(3107..3528)
                     /locus_tag="ACHOSM_RS24280"
                     /old_locus_tag="pgaptmp_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          880 a          852 c          938 g          858 t
ORIGIN      
        1 gtaaaaggca aatataaatt attctcatta ttgtttgtgt ttgtgtattg tcttgccggt
       61 taacatgatc ggagattagt aatatgcgaa taaccactct ggcttccgta gtcattccct
      121 gtctcggatt ttcagccagc agcatagctg ctgcagagga tgtgatgatt gtctcggcat
      181 ccggctatga gaaaaagctg actaacgcag ccgccagtgt ttctgtgatt agccaggagg
      241 aattgcagtc cagccagtac cacgatctgg cggaggctct gagatcagta gagggtgtgg
      301 atgttgaaag tggtacgggt aaaaccggag ggctggaaat cagcatccga ggaatgccag
      361 ccagttacac gctgatactg attgatggtg ttcgtcaggg cggaagcagt gacgtgactc
      421 ccaacggttt ttctgccatg aataccgggt tcatgccccc tctggccgcc attgagcgta
      481 ttgaggttat cagggggccg atgtccacac tgtatggctc tgatgcgatg ggcggtgtgg
      541 tgaatatcat taccagaaag aatgcagaca aatggctctc ttccgtcaat gcagggctga
      601 atctgcagga aagcaacaaa tggggtaaca gcagccagtt taatttctgg agcagtggtc
      661 cccttgtgga tgattctgtc agcctgcagg tacgcggtag cacacaacag cgtcagggct
      721 catcggtcac atcactgagc gatacagcag ccacgcgtat tccttttccc acggagtcac
      781 agaattataa tcttggtgca cgtcttgact ggaaggcgtc ggagcaggat gtgctctggt
      841 ttgatatgga taccacccgg cagcgttatg ataaccggga tgggcaactg gggagtctga
      901 cggggggata tgaccggacc ctgcgctatg agcgaaacaa aatttcagct ggctatgatc
      961 atactttcac cttcggaaca tggaaatcgt atctgaactg gaacgagaca gaaaataaag
     1021 gtcgtgagct tgtacgcagt gtactgaagc gcgacaaatg ggggcttgcc ggtcagccgc
     1081 gggagcttaa ggaatcgaac cttatcctga attcattact gcttacccct ctgggagaat
     1141 ctcatctggt tactgtgggg ggcgagtttc agagctcgtc catgaaagac ggagttgtcc
     1201 ttgccagcac aggtgaaact ttccggcaga aaagctggtc ggtatttgct gaggatgagt
     1261 ggcatctcac ggatgcactt gcgctgactg cgggcagccg ctatgaacat catgagcaat
     1321 tcgggggaca cttcagtccg cgtgcatatc tggtctggga tgtggcagat gcctggacgc
     1381 tgaaagtcgg tgtgaccacg ggatataagg cacccagaat ggggcagcta cataaaggga
     1441 ttagtggtgt gtccgggcag ggaaaaacaa atctacttgg taaccccgac ctgaagccgg
     1501 aagagagcgt cagttatgag gctggggtgt attacgataa ccccgccggt ctgaatgcca
     1561 atgtcacagg ttttatgact gacttctcca acaagattgt ctcttattcc ataaatgata
     1621 acaccaatag ctatgtaaac agcggaaagg cccggttgca cggtgtggaa tttgccggca
     1681 cattgccgct gtggtcagag gatgtcacgc tgtcactgaa ttacacctgg acccgaagtg
     1741 aacaacgtga tggtgataac aaaggtgcgc cgctgagtta tacccctgaa cacatggtga
     1801 atgcgaaact gaactggcag atcaccgaag aggtggcatc atggctgggt gcccgttatc
     1861 gcgggaaaac accacgtttc acccagaatt attcgtcact gagcgctgta cagaagaaag
     1921 tgtatgatga gaaaggagaa tacctgaaag cctggacggt ggtggatgca ggtctgtcgt
     1981 ggaagatgac ggatgccctg acgctgaatg ctgcggtgaa taacctgctc aacaaggatt
     2041 acagtgacgt gagcctgtac agtgccggta agagtacgct gtatgccggt gattacttcc
     2101 agacgggatc atcaacaaca ggatatgtga tacctgagcg aaattactgg atgtcgctga
     2161 actatcagtt ctgataataa caaaacgcta tcactgacgg tagaatacgt tgccactgca
     2221 actcctggcg gaacagtggc aacgtattag gttaagtgca tttccgatcc gctaatgaga
     2281 tttcgttacc aacaactaat atcgtcacag gaaatgcacg gattattttt aacttatcat
     2341 ttacatactt gtccagagtg taagcgcacc gtgaaggacg tggggtaaaa attagtttac
     2401 agagagagtg acgttccagg gcaacaactc tttcatgcgg ttggcaggcc tggtgttgat
     2461 tacactgatc acgtggcgta gccacgtttc cggctcgatt ccgttaagtt ttgcagctac
     2521 cgatcaggct gtacatcact gccgcactat cgctcgtcat ctcaaagtcc ggtctcgtca
     2581 gcaggaaggt atcattctct cccgccattt ttccaggggc ccggtcagat aagtcccttt
     2641 gtctatcgct gactcctgac tcataacccg gttagcagaa tgcagggtca ccacctcgcc
     2701 acgaccgaat ccgaataagt caattgcacc ttctcaatcg ccattttgtc acaattaagt
     2761 ttgataaagc aggtcacctc tgcactgaat ctgctccagt gcctcattgg tttccgtccc
     2821 cccctttaaa ctgccggata agactgctgc acagcgcctt ccccggaatc aaatcacttt
     2881 ccctgtccgc cacaatccgc ttcatgccac ctcgtatttt gtttcagaga ccggaatctg
     2941 ccagaaagtg actacgcgtg cacatatcgg ttttctgggt tacatgcatc gtgttatttt
     3001 ctgaaatgca aaataataaa aaacaaggac acataatgaa ggatgaaata cacagtacca
     3061 gcatcaaccg taagcgtaca gagagggccg tattgacggg gatgtgttat tcagctggca
     3121 gtgctatgcg ccacggaagc agttcgctga cccggtttat cggccagtcg gctatgacgt
     3181 caaggacata gcgtaggtag ctttctggct ccactccgtt cagtttgcac gtcccgatca
     3241 ggctgtacag cagcgctccc cgctctcctc catgatccga accgaagaac aggtaatttt
     3301 tgcggcccag actgaccatc cgcagcgcat tctcagctat gttgttatca gcctcggccc
     3361 agccgtcgtc agtatagtaa gccagcgctg gccactggtt cagtacgtac gtgaacgctt
     3421 tcgccagttc tgagtgtcgc gacagcgttt tcaccttttc acgtagccag ccttccaggg
     3481 atttcagtcg cctttgtcat cgcgggccga cggccaggcg aagcggcc
//
LOCUS       DAZKHC020000230         3489 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZKHC020000230 DAZKHC020000000
VERSION     DAZKHC020000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.231"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(413..648)
                     /locus_tag="ACHOSM_RS24285"
                     /old_locus_tag="pgaptmp_004857"
                     /pseudo
     CDS             complement(413..648)
                     /locus_tag="ACHOSM_RS24285"
                     /old_locus_tag="pgaptmp_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(819..1469)
                     /locus_tag="ACHOSM_RS24290"
                     /old_locus_tag="pgaptmp_004858"
     CDS             complement(819..1469)
                     /locus_tag="ACHOSM_RS24290"
                     /old_locus_tag="pgaptmp_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGU4561176.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLDVFSTYMGQVIS
                     EFMHSNDNRIELLQQRLHSCSFLVNIEEMSYIDEALQCPITLAIPQRGVFLRNAEGSR
                     VCSLYDEMALSRIINDGMHHPLSREPITLSMLVAREQCEFDCSIGHFTVRSDCYSE"
     gene            1701..2757
                     /locus_tag="ACHOSM_RS24295"
                     /old_locus_tag="pgaptmp_004859"
                     /pseudo
     CDS             1701..2757
                     /locus_tag="ACHOSM_RS24295"
                     /old_locus_tag="pgaptmp_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(2976..>3489)
                     /gene="espM1"
                     /locus_tag="ACHOSM_RS24300"
                     /old_locus_tag="pgaptmp_004860"
     CDS             complement(2976..>3489)
                     /gene="espM1"
                     /locus_tag="ACHOSM_RS24300"
                     /old_locus_tag="pgaptmp_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HGU4561177.1"
                     /translation="IRGKNDEVMKCSMGERSIRFNVNKFSGCILETVSRQRTKDIHGW
                     VSDERTVYPSRVINQEIDNCCLQKNAKISSEERKMVFSLVSKEFELTLDVKAAQSSIN
                     HIIIGNASFGKKMDALCDGMSRAVKNSTTDYIANVLADKFYQKHIAPGVDIVKLRNEI
                     PGYMSRVIQG"
BASE COUNT         1047 a          812 c          654 g          976 t
ORIGIN      
        1 gggctattta aacaatcact aaaaatcgcc caagagcatt ctgtttctat aatgtattcc
       61 aagagattaa aaaataatat catacgttat tcggcagagt tcgtatgtac tacggcgtcc
      121 caaccatgtc ccaaaacaaa aatttctgcc ctatacacgt tccaaaaaat tccacaacaa
      181 agagagtctg ctgacgagtt gttgattcac tgattaatac gtaaaattga taactgctgg
      241 aaatcataca atactcgtac tttcgaaagt tcgctaacca ggtgctacac gcgaggtgtt
      301 gcagttttct ttacgattta tcattcaatc ctaatccatg catgacatgt ggtgcctgtg
      361 gggtgatcct acctacgtaa tgtggacaca gccctaatca aggttctgtt tttcaaaaca
      421 gttccgtgct gagacagcca caagtactgt gtcggcacca ccgattgtaa tcacactaga
      481 tctaattaaa cacagccgtc tgcattattt cccgactcat aaaatattag ccgtaaatgc
      541 attcgacttt tagtgagtgg aagaaagttt ccgccctagc attatcgtaa cagcgcccct
      601 ttgcactcat cctgtcatgc aggtatgccg cttcagaagc gcctgataat gcgttgaaat
      661 caggacggtg gctacttgct attcagttca cgatacaaaa tgggcaatat cccttaagga
      721 ccagcgaata gcaatgagtc actttattat caagtttgat gaccgctttg atgctaactt
      781 ttaataagag tctcttcaca caaaatcgtg taccgagtct actctgaata acaatcactc
      841 ctcaccgtaa agtgaccgat actgcaatca aactcacact gctctctggc cacaagcatt
      901 gataatgtta ttggctctct gcttagtggg tgatgcatcc catcattaat tatacgagaa
      961 agagccattt catcatataa actacatact ctggaacctt cagcatttct taaaaaaaca
     1021 cctcgttgag gaattgccag cgtaatcggg cactgtaatg cttcatctat gtaagacatt
     1081 tcttcaatat taactaaaaa tgaacatgaa tgtaatcgct gctgtaacaa ttcaattctg
     1141 ttatcattac tatgcataaa ttcagaaata acctgcccca tatatgtact gaatacatca
     1201 agaaatgaat tacctccatt caattgattt tcaacagaat cagccaatct aatgatatcg
     1261 gctagttctg tatggcttaa acccgaatat agttcccctt gaacaggatc tacaataaaa
     1321 gcgtccatag cactctgata gcgaacagaa aacgatcttt ctcctattgt gagtctttcc
     1381 atttgatgat ctctggtcgc acgttgtaaa acttcaacat cttgtgttga caagtaattt
     1441 ccagaagaca aattaaatga aaatggcata attttatccc taaagcatat atcttacttt
     1501 tcgataatta acaaaaagcc atcctccaca gcaatactcc atcagaataa ttgtaggatt
     1561 attttaatct aatttataag cataaaatat atactcacta aatagctgcg cagaatagta
     1621 gatcatttag agggaactca acccgattgt gcgatctgat caatcgccaa atcaatacaa
     1681 atcaccaacc ggactgagca atgtcgatca taccaccaat ttcccgtgac gaacgacgcc
     1741 taatacagaa agccatccat aaaaggctga cagccatgct gatgctgcac cggggcgacc
     1801 gtgtcagcga cgttgcccgc acactatgct gcgcccgttc ctctgttggg cgctggatta
     1861 actagttcac acagtcgggt gttgagggac tgaaatcatt acctgccgag cgagcccgcc
     1921 gcaggccgtt tgcgcatatc tacacactat tacgtgaaca ggtaaaatat tcactcgcga
     1981 ctttggctac aattattcac gctggagtac agaactgctg acaataaaaa tcaatgagat
     2041 aaccggctgc ctgttacatg ctggaaccat tcgccgctgg ttgtcgtctg tggggcttgt
     2101 gtggcgaagg gctgcgccaa ctctacgtat ccgtgccccg cctaaagata aaaagatggc
     2161 aacaatccat aacgcactgg acgaatgcag cacagagcat ccggtctttt atgaagatga
     2221 agtatttatc catcttaatc ccaaaatcgg tgcggactgg aaactgctcg ggaagcaaaa
     2281 acgaggggtc acgccggaac aaaatgaaaa atattctctg gacgtagcgc tgcacagcgg
     2341 gacaggttaa gtcagctgtg tgggcgccaa cagcaaaagt tcggcgctgt tcatcagact
     2401 gctgaagcgg cttaaagcta catactgtcg gacgaaaacc atcacgctgc tcgaggagaa
     2461 ctacattatc cacaaaggcc gggaaaccca gcgctggctg aaggataaac cgaagtgcag
     2521 agtcatttat cagccggttt aatctccatg ggtgaatcat tttgaatagc tatggcagat
     2581 acttcacgcc acaataacgc gtaatcatca gtgccgctca atgtggcaac tattgaaaaa
     2641 gttcgccatt ttatggaatc cgtcagtcca ttccccgaag gcaaacatgg gctggcaaaa
     2701 atgtgcgata ttaggcgcag ctatttagtt attccgtcat ttctcccgcc cgtgtaaaca
     2761 ctactgaatc ttgtcgttgt gacaaagcta taaatagtca atcacagcac gtttctgcgt
     2821 acgacgcgct gcaattttct ttacgattta taactgtaca ggtgaactat gagttatttg
     2881 gatacataca tatagtgtaa cgcctgttgt atattttact catatctttt tacaggatga
     2941 aaaccaatga tactggcgca tagcctcctc caaccttacc cctgtataac acgactcata
     3001 taacctggga tttcatttcg tagttttaca atatcaacac ccggggcgat atgtttctga
     3061 taaaacttgt ctgcaagcac gtttgctatg taatctgttg tactgttttt tacagctcgg
     3121 ctcataccat cgcaaagagc atccattttt ttgccaaaag aagcatttcc tattattata
     3181 tgattaatag aactttgtgc cgctttaaca tcaagagtta gttcaaactc cttacttaca
     3241 agagaaaaga ccattttcct ctcttcagaa gaaatttttg catttttttg aaggcagcaa
     3301 ttatcaatct cttggttgat cacccttgac ggatatactg tccgttcatc acttacccat
     3361 ccatgaatat ctttagtacg ctgcctgctt accgtctcca gtatacagcc gctaaatttg
     3421 ttaacattaa agcggataga gcgctctccc attgaacact tcatgacctc atcattcttc
     3481 ccacggatg
//
LOCUS       DAZKHC020000231         3296 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZKHC020000231 DAZKHC020000000
VERSION     DAZKHC020000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.232"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            160..1680
                     /gene="yifB"
                     /locus_tag="ACHOSM_RS24305"
                     /old_locus_tag="pgaptmp_004861"
     CDS             160..1680
                     /gene="yifB"
                     /locus_tag="ACHOSM_RS24305"
                     /old_locus_tag="pgaptmp_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HGU4561178.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1705..2043)
                     /gene="maoP"
                     /locus_tag="ACHOSM_RS24310"
                     /old_locus_tag="pgaptmp_004862"
     CDS             complement(1705..2043)
                     /gene="maoP"
                     /locus_tag="ACHOSM_RS24310"
                     /old_locus_tag="pgaptmp_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HGU4561179.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2162..2998
                     /gene="hdfR"
                     /locus_tag="ACHOSM_RS24315"
                     /old_locus_tag="pgaptmp_004863"
                     /pseudo
     CDS             2162..2998
                     /gene="hdfR"
                     /locus_tag="ACHOSM_RS24315"
                     /old_locus_tag="pgaptmp_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
     gene            complement(3097..3172)
                     /locus_tag="ACHOSM_RS24320"
                     /old_locus_tag="pgaptmp_004864"
     tRNA            complement(3097..3172)
                     /locus_tag="ACHOSM_RS24320"
                     /old_locus_tag="pgaptmp_004864"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3137..3139),aa:Trp,seq:cca)
     gene            complement(3181..3257)
                     /locus_tag="ACHOSM_RS24325"
                     /old_locus_tag="pgaptmp_004865"
     tRNA            complement(3181..3257)
                     /locus_tag="ACHOSM_RS24325"
                     /old_locus_tag="pgaptmp_004865"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3221..3223),aa:Asp,seq:gtc)
BASE COUNT          883 a          816 c          808 g          789 t
ORIGIN      
        1 ttcatccttt gtccgctgca tttttgcgag catcattcca ccgccagaac aactcaactt
       61 cacttgcaca aataaacagg aatcagccac taaaattcac gaattagcgg gctacagcag
      121 cgatatctta tgcacataat ggtgactcaa ggagggctta tgtcactgtc aattgttcat
      181 acccgcgcag ccctgggagt aaatgcgccc ccgatcactg ttgaggtaca tatcagtaaa
      241 ggtctacccg gcttaacgat ggtgggctta ccagaaacaa cggtaaaaga agctcgcgat
      301 cgcgtgcgca gcgccattat caatagcgga tatgaatatc cggcgaaaaa aatcaccatc
      361 aacctggctc cagctgatct gccaaaagaa gggggacgat atgatttacc tatcgccatt
      421 gcgttgctgg cggcctcaga acagcttaca gccaataagt tagatgaata tgaattagtc
      481 ggagaactgg cgcttacagg cgctctgcgt ggcgttcccg gcgcaatctc cagtgcaact
      541 gaagctatta agtcgggcag aaaaattatc gtcgcgaaag ataacgaaga tgaagtgggg
      601 ctaattaacg gtgaaggatg cctgatagcc gatcatctgc aagctgtctg tgcgtttctg
      661 gaaggtaagc acgctctcga acgcccgaaa ccaactgatg cagtatcccg ggcgctacaa
      721 catgatctca gtgatgttgt cggtcaggaa caaggaaagc gaggactgga aattaccgcc
      781 gctggcgggc acaacctttt actgattggg ccgccgggaa caggtaaaac aatgctcgcc
      841 agccgtatta atggcctgtt gccagattta agcaatgaag aggcactgga gagtgctgcg
      901 atattaagtc tggtaaatgc tgaatcagta caaaaacaat ggcggcagcg cccgttccgc
      961 tcacctcatc acagtgcatc gttaactgcg atggtaggcg gtggcgcaat tccagggccc
     1021 ggtgaaattt cgctggcgca taacggcgtg ctttttcttg atgagttacc tgaatttgaa
     1081 aggcgtacac tggatgcctt gcgagagccg attgaatccg ggcagatcca tctttcacgc
     1141 acgcgagcaa aaataaccta tccagcccgt ttccagcttg tcgcggcgat gaatcccagc
     1201 cctaccggac attatcaggg aaaccataac cgctgcacac cagagcaaac gctgcgttat
     1261 ctcaaccgac tctctggtcc ctttctcgac cgcttcgatc tctcactgga gatcccatta
     1321 ccgcctcccg gtattttgag taaaacggta gtgccgggag aaagcagcgc caccgttaaa
     1381 caacgcgtaa tggccgccag agagcgccaa tttaagcggc agaataaact gaacgcctgg
     1441 ctggatagtc cggaaatacg ccaattctgc aaacttgaga gcgaagatgc gatgtggctg
     1501 gaagaaacgc tgatccatct ggggttatcg attcgtgcct ggcagcggtt attgaaagtt
     1561 gcacgaacca ttgctgatat tgatcagtct gacattatca cacgtcaaca tttgcaggag
     1621 gcagttagct atcgagcgat tgaccgtttg ctcatccatc tgcagaaact actgacataa
     1681 aaaaagggca tttcgccctt tttattaatc gtcagaatcg gtgtagtctt cagcaccttc
     1741 aacctgcggt ttaccgccgg aaagggtgtg aaaacgtttt ggacgcttaa tacgcgtcat
     1801 atacttggac cacacgcgtt ctgcttctgt cactggctca cgttcgccac ggcatactgc
     1861 tacgaagagt ttctcttcct cggtaaccgg ctcgcgtttg ccaagatcca actcattgaa
     1921 ggcataacca tgacgctcaa gcagttgtgc ctctttgatg gtgaaatcac catgacgaga
     1981 gaatccacgt ggataatgtt tattgtcgaa atatcgatta gtcgtcgtaa agctttccgc
     2041 catcctgcac gctcctaatt ctttgaccga gctagttatg gcgcggagta ttagttacgc
     2101 ttgacagagt gtaaaacaaa acatttaaat cataacgaca aataattttg cggagagcac
     2161 tgtggatacg gaattgttaa aaactttcct ggaagttagc cgaacgcgtc actttggtcg
     2221 agcggctgaa tcgctctatc tgacccagtc agcagtgagc tttcgaatca gacaactgga
     2281 aaatcaactg ggtgtgaacc ttttcacccg ccacagaaac aatatccgtt taaccgctgc
     2341 cggtgaaaaa ctactgcctt atgcagaaac gctcatgagc acctggcagg ccgcccgcaa
     2401 ggaggtggcg catacctcac gacataacga gttttctatc ggtgccagcg cctcattgtg
     2461 ggaatgtatg cttaatcagt ggctgggacg tttgtatcaa aatcaggatg cccatacagg
     2521 cttacagttc gaagcgcgaa ttgcccaacg gcagtctctg gtaaaacagc tgcatgaacg
     2581 ccagcttgat cttcttatta ccactgaagc gcccaaaatg gacgaattta gtagtcagtt
     2641 gctgggatat ttcactttag cgctttatac cagtgcccct tccaaactaa agggagatct
     2701 taactatctg cgactggagt gggggccaga ttttcaacag catgaggcag gtttgatctg
     2761 tgctgacgaa gttcccattc tgacaaccag ttctgctgaa ctggcacagc aacagattgc
     2821 gatgcttaat ggttgcacct ggctacccgt cagctgggcg cgtaaaaaag gcggcctgca
     2881 tactgttgtt gatagcacaa cactttcacg accgctttat gccatatggc tgcaaaatag
     2941 cgataaaaat gcgttgattc gcgatctatt gaaaattaac gtgctggatg aagtgtatga
     3001 atctgaatgg ctggcaagga tgccggtaga aggatttact tcggagagga ttatttcaga
     3061 taaaaaaaat ccttagcttt cgctaaggat gatttctggc aggggcggag agactcgaac
     3121 tcccaacacc cggttttgga gaccggtgct ctaccaattg aactacgccc ctaattaggg
     3181 tggcggaacg gacgggactc gaacccgcga ccccctgcgt gacaggcagg tattctaacc
     3241 gactgaacta ccgctccacc gaattctttt acaaccaccg gtttcatgac cggctt
//
LOCUS       DAZKHC020000232         3295 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZKHC020000232 DAZKHC020000000
VERSION     DAZKHC020000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.233"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            78..539
                     /gene="yegJ"
                     /locus_tag="ACHOSM_RS24330"
                     /old_locus_tag="pgaptmp_004866"
     CDS             78..539
                     /gene="yegJ"
                     /locus_tag="ACHOSM_RS24330"
                     /old_locus_tag="pgaptmp_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HGU4561180.1"
                     /translation="MKKILLMLSLLFFTTAGFSEVSDTLVTGGYDKQAMSDAIKHARK
                     ETDQFIEVMNKKDADTFAVKAPITDHGRTEHFWLTDVTYSNGMFIGVISNDPGIVTNV
                     EYGQEWKIKKEDISDWMYTRGDKIYGGYTIDPLLVTYPKEEADELRAKLVR"
     gene            complement(604..1365)
                     /gene="pphC"
                     /locus_tag="ACHOSM_RS24335"
                     /old_locus_tag="pgaptmp_004867"
     CDS             complement(604..1365)
                     /gene="pphC"
                     /locus_tag="ACHOSM_RS24335"
                     /old_locus_tag="pgaptmp_004867"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HGU4561181.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWAE"
     gene            complement(1362..2021)
                     /gene="yegL"
                     /locus_tag="ACHOSM_RS24340"
                     /old_locus_tag="pgaptmp_004868"
     CDS             complement(1362..2021)
                     /gene="yegL"
                     /locus_tag="ACHOSM_RS24340"
                     /old_locus_tag="pgaptmp_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU4561182.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(2242..2301)
                     /gene="ibsA"
                     /locus_tag="ACHOSM_RS24345"
                     /old_locus_tag="pgaptmp_004869"
     CDS             complement(2242..2301)
                     /gene="ibsA"
                     /locus_tag="ACHOSM_RS24345"
                     /old_locus_tag="pgaptmp_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HGU4561183.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(2574..2630)
                     /locus_tag="ACHOSM_RS24350"
                     /old_locus_tag="pgaptmp_004870"
     CDS             complement(2574..2630)
                     /locus_tag="ACHOSM_RS24350"
                     /old_locus_tag="pgaptmp_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4561184.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            3051..>3295
                     /locus_tag="ACHOSM_RS24355"
                     /old_locus_tag="pgaptmp_004871"
     CDS             3051..>3295
                     /locus_tag="ACHOSM_RS24355"
                     /old_locus_tag="pgaptmp_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspJ"
                     /protein_id="HGU4561185.1"
                     /translation="MPIIKNCLSLIGNALRNETTSYSLTKTEQVGKMLNIKIIPTPTP
                     VKLLSYRNADLIKENHTTEKVLSIFNIKRDFVAVRIQ"
BASE COUNT         1007 a          765 c          697 g          826 t
ORIGIN      
        1 attcaatttc ggcctgataa tgattaactc ccacaggttt ttgctaactt tgactacaaa
       61 gggattctgg agaaaaaatg aaaaagatat tacttatgtt gagtctgcta ttttttacta
      121 ccgcgggctt tagtgaagtt tcggatacgt tagtgacggg aggttatgat aaacaagcga
      181 tgtctgacgc gattaaacat gcgcgtaaag aaactgatca attcatcgaa gttatgaata
      241 aaaaagatgc cgatactttc gcagtcaaag ccccgataac cgatcacgga agaaccgagc
      301 atttctggct cacggatgtc acttattcta acggcatgtt tattggcgta atcagcaatg
      361 accctggtat tgttacaaac gtagaatatg gccaggagtg gaaaatcaag aaagaagata
      421 tttcagactg gatgtatacg cgcggggata aaatttacgg cggttatacc attgatcctt
      481 tattggtcac ctatccaaaa gaagaagccg acgagcttag agcaaaatta gttcgctaat
      541 cagccgttat atttattgtt taaactggcg gttgaattgt tgtcttcaac cgccatgaga
      601 aactcactct gcccacagcg ccagtgctaa cgttttatca tcatcggtac gctcgtttac
      661 cgctgggctg gacaaaaact gctttaacaa ttcaggtaat aaatcgagtt gttcctgcgt
      721 tgctgacgcc agtccattga aaaacggggt aaagaaaggc acatgcggag aattatccaa
      781 catatttaac gccagtcgct ggataccatc cgtaaatgct gcaactttat gcacacgctc
      841 agtgctggtg aaagtttcca gtctggaaac ggcatcttca tcggtaatga agtgcgtcat
      901 attggcatat tcacccacca tcggcgtaag cggcaattgc agaccgtgac caaaatccac
      961 caccacgccg ccatcgccaa tctgcataat caaggtgcca ttagccgatg aaatcaatcc
     1021 cagaaacgtg caggcaaaat cgcgaactgc taattctttg gcttccgcct cggcaaaaag
     1081 tcgctgacga atagtcagta ccatatttgt ggcgaggaca tcattaagcc ccaattcacc
     1141 gtcctgtact ttttgcacca taaacgccat cgcttcattg acggcgagcg tcgcgccttc
     1201 tcctccctgc gaaacgctac ctgcgccatc agcaaggaac atcaccagta atggttgctg
     1261 gtcgtttaac cagacaacct gcatctggca ggcatcctga caaggcaaat cagcactaat
     1321 gtgcgatgtt ccaacagcgg atgcataaac cagacgccag ctcacactga cgtccagcct
     1381 tttggcgctt ccagcacaac ttccgttccc ggcgtggagc gggaaaccga acgcagcgaa
     1441 ctggataacc agctaaacag ttcacggaat tgtaatcctt gcagaggcaa aggctgacga
     1501 acgctgattt gtgccagagt cttcatatcc gccccctgaa caccgatgga aaagaaagca
     1561 aaccttttat cttcttcccc ccgaaacact ttgttggctg cagcctgcca ctcatcggtt
     1621 ggtgcaccat cggtaatcag gaaaatccac gggcgataat acgaaatacc atttgcacga
     1681 tactcacgtt tccgctcctc taccatatcc agggctttgg taatggccgc acccattggt
     1741 gtatcgccct gggcaaacag gatgggcgga aaaaaattag cggcgctggt aaagggttgt
     1801 tccacatgca ccgggccgaa cgtcacaatg ccgagttcca ctctttttaa agccagcgga
     1861 tcagcaagca gttcatcacg aaaggtaacc aatccggcgt taagttcatt aataggtcta
     1921 ccgttcatag aaccagaaac atccagcagc aaaatgcagg ggcaacgtgg ttccgggtta
     1981 ctggcaaaat cgctggtggc aaacgtgatt tgttcgctca taagaaatcc tttcttagag
     2041 aattatcgag gtccggtatc ataccgactg caataatgaa ataagagcaa acgaagaaac
     2101 tttcagaatt aggcccggcg acgcagaaaa ttttgctatt cgcctcgcaa gaacggagag
     2161 ccgtcgattg acactaaggg cggagtgaca taatttcagg agtgagggtt agggagaggt
     2221 ttccccctcc ccctggtgtt cttagtaagc ctggaagcta atcactaaga gtatcaccag
     2281 tatgatgacg tgcttcatca taaccctttc cttattaaaa gccctcttct ccgggagagg
     2341 ctttcccgtt tcagcgtccc gctgaaatca tcggcttacc tcctttcgcc ctgaatgcag
     2401 tctatcgcta acgcgtgaat aaaatagttt cctctgtgat tattgcgtaa cgactcgcaa
     2461 agaatatttg ctggcaggat cgcagactac aaagcctgcg gattgacaat cttatcgtga
     2521 aggcatactt tcaggagtga gggtagagcg gggtttcccc cgccctggta gtcttagtaa
     2581 gcggggaagc ttatgactaa gagcaccacg atgatgagta gcttcatcat gaccctttcc
     2641 ttattaaagg atgacaaata atataaatca cttaagaatc atcgcattac acgaatccac
     2701 gattaaataa tagactgcaa aatgcgacaa aactcaacat atgcagtcac tatgaatcag
     2761 ctacttagat ggtattagtg acctaagaca gagcattagc gcaaggtgat tttttgtcct
     2821 cttgcgctaa ttttttgaca taaaaacatg tggcagactt tgcgtaactc tgctttgcca
     2881 cgcaacatca tcatgcatta tggctatgcc taaacagaat caataagaat gaaatataaa
     2941 aaacacaact actagacaat taaccacaaa aatgataaca actaatttag tttgtacaat
     3001 ataagacttc ccatcaaaag ttcatccata tggatgacaa ggagtttatc atgccaatta
     3061 taaagaactg cttatcatta attggtaatg cattaagaaa cgaaacaaca tcatattccc
     3121 taacaaaaac agaacaggtg ggaaagatgt taaatataaa aataatccca acacccacac
     3181 cggtgaagct attatcatat cgaaatgctg atttaataaa agaaaaccac actacagaaa
     3241 aagttttatc tatttttaat ataaaacggg atttcgttgc tgtaagaatc caaag
//
LOCUS       DAZKHC020000233         3274 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZKHC020000233 DAZKHC020000000
VERSION     DAZKHC020000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.234"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..524
                     /gene="yibB"
                     /locus_tag="ACHOSM_RS24360"
                     /old_locus_tag="pgaptmp_004872"
     CDS             <1..524
                     /gene="yibB"
                     /locus_tag="ACHOSM_RS24360"
                     /old_locus_tag="pgaptmp_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HGU4561186.1"
                     /translation="WSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVT
                     RGLKIWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEF
                     YKLVLESQKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLG
                     AKMQALRIFLSRK"
     gene            799..1995
                     /gene="kbl"
                     /locus_tag="ACHOSM_RS24365"
                     /old_locus_tag="pgaptmp_004873"
     CDS             799..1995
                     /gene="kbl"
                     /locus_tag="ACHOSM_RS24365"
                     /old_locus_tag="pgaptmp_004873"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HGU4561187.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            2005..3030
                     /gene="tdh"
                     /locus_tag="ACHOSM_RS24370"
                     /old_locus_tag="pgaptmp_004874"
     CDS             2005..3030
                     /gene="tdh"
                     /locus_tag="ACHOSM_RS24370"
                     /old_locus_tag="pgaptmp_004874"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HGU4561188.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
BASE COUNT          801 a          717 c          867 g          889 t
ORIGIN      
        1 actggtcacc agagtatgtt ttggtatgta accttaaggc ctattttgta aataaagcta
       61 tcaacatggg cttagtgaaa acgcctcttg ttgcgtggat agattttggc tattgccgta
      121 agccaaatgt aactcgagga ttgaaaattt gggatttccc atttgatgaa agtaagatgc
      181 atcttttcac tataaaaaaa gggcttactg tcacatctca acaacaggtt tttgatttca
      241 tgattggcaa tcatgtatat attattgggg gcgcaattgt tggttcacag cataaatgga
      301 aagaattcta taaattagtt ctggaaagtc aaaaaataac actaaataat aacattgtgg
      361 acgatgacca gggcattttt gtcatgtgct attacaaacg ccccgatctc ttcaacctaa
      421 actatctggg gagaggaaaa tggttcgatt tgttccgctg cttcaggagt aacactttag
      481 gggcaaaaat gcaggcgctg agaattttcc tttcaagaaa ataggtttcc ctccattagt
      541 gggtagcgtt ttgataccaa ctgagatagg aaaaatgatg ctttggcatc attttttctt
      601 tttatatcaa tgcagtaaaa tcaatcatca ttatgcccag ccaaaactgt acagtttttc
      661 tactatctgt aggtgttgag agacatcaag ttacacgtta tttatcctga attttgcaga
      721 agtgttaacg cgtcatctcg tcgcgacgta taagtttggg taatatgtgc tggaatttgc
      781 cctgtctgga gaatcgcaat gcgtggagaa ttttatcagc agttaaccaa cgatctggaa
      841 accgcacggg cggaagggtt gtttaaagaa gagcgcatta ttacgtctgc gcagcaagca
      901 gatatcactg tggctgatgg aagccacgtc attaactttt gtgccaacaa ctatctcggg
      961 ctggcgaatc atccggatct gattgcggca gcaaaggcgg gaatggattc tcacggtttc
     1021 ggcatggctt cggtgcgttt tatttgcggc actcaggaca gccataaaga gcttgaacaa
     1081 aaactggcgg cattcctggg gatggaagat gcgattctct actcttcctg ctttgatgct
     1141 aacggtggac tgtttgaaac gctgctgggt gcggaagacg ccattatctc cgacgcactg
     1201 aaccacgcgt ctattattga tggtgtgcgt ctgtgcaaag ctaaacgcta tcgctatgcc
     1261 aacaacgata tgcaggagct ggaagcacgt ctgaaagaag cgcgtgaagc cggtgcgcgt
     1321 catgtgctga ttgccaccga tggtgtgttc tcaatggacg gcgtgattgc caacctgaaa
     1381 ggcgtttgcg atctggcaga taaatacgat gccctggtga tggtggatga ctcccacgcg
     1441 gtcggttttg tcggtgaaaa tggtcgtggt tcccatgaat actgcgatgt gatgggccgg
     1501 gtcgatatta tcaccggtac gcttggtaaa gcgctgggcg gggcttctgg tggttatacc
     1561 gcggcgcgca aagaggtggt tgagtggctg cgccagcgtt ctcgtccgta cctgttctcc
     1621 aactcgctgg caccggccat tgttgccgcg tccatcaaag tactggagat ggtcgaagcg
     1681 ggcagcgaac tgcgtgaccg tctgtgggcg aacgcgcgtc agttccgtga gcaaatgtcg
     1741 gcggcgggct ttaccctggc gggagccgat catgccatta ttccggtcat gcttggcgat
     1801 gcagtagtgg cgcagaaatt tgcccgtgag ctgcaaaaag agggcattta cgtcaccggt
     1861 ttcttctatc cggtcgttcc gaaaggtcag gcgcgtattc gtacccagat gtctgcggcg
     1921 catacccctg agcaaattac gcgtgcagta gaagcattca cgcgtattgg taaacaactg
     1981 ggcgttatcg cctgaggatg tgagatgaaa gcgttatcca aactgaaagc ggaagagggc
     2041 atctggatga ccgacgttcc tgtaccggaa ctcgggcata acgatctgct gattaaaatc
     2101 cgtaaaacag ccatctgcgg gactgacgtt cacatctata actgggatga gtggtcgcaa
     2161 aaaaccatcc cggtgccgat ggtcgtaggc catgaatatg tcggtgaagt ggtaggtatt
     2221 ggtcaggaag tgaaaggctt caaaatcggc gatcgcgttt ctggcgaagg ccatatcacc
     2281 tgcggtcact gtcgcaactg tcgtggcggt cgtacccatc tgtgccgcaa taccattggt
     2341 gtcggtgtta accgtccggg ctgcttcgcc gaatatctgg tgattccggc gttcaacgct
     2401 ttcaaaatcc ccgacaatat ctccgacgac ctggcttcca tttttgatcc cttcggtaac
     2461 gccgtgcata ccgcgctgtc gttcgatctg gtgggcgaag atgtgctggt ttctggtgca
     2521 ggtccgatag gtattatggc tgcggcggtg gcgaaacacg ttggtgcacg caatgtggtg
     2581 atcaccgatg ttaacgaata ccgccttgag ctggcgcgca aaatgggtat cacccgtgcg
     2641 gttaacgtcg cgaaagaaaa ccttaatgat gtgatggctg aactgggcat gaccgaaggc
     2701 tttgatgtcg gtctggaaat gtccggcgcg ccgccagcgt ttcgtaccat gctcgatacc
     2761 atgaaccacg gcggtcgtat tgcgatgctg ggtattccac cgtctgacat gtctatcgac
     2821 tggaccaaag tgatctttaa aggcttgttc attaaaggca tttacggtcg tgagatgttt
     2881 gaaacctggt acaagatggc ggcgctgatt cagtctggcc tcgatctctc gccgatcatt
     2941 acccatcgtt tctctatcga tgatttccag aagggctttg acgctatgcg ttcgggccag
     3001 tccgggaaag ttattctgag ctgggattaa cacgaacaag ggctggtatt ccagcccttt
     3061 tatctgagga taatctgtta attatgtaaa atcctgtcag tgtaataaag agttcgtaat
     3121 tgcgctgatc ttttatatag ctgttctcat tatctctcta ccctgaagtg actctctcac
     3181 ctgtaaaaat aatatctcac aggcttaata gtttcttaat acaaagcctg taaaacgtca
     3241 ggataacttc agaggtcgtc ggtaatttat gatg
//
LOCUS       DAZKHC020000234         3270 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZKHC020000234 DAZKHC020000000
VERSION     DAZKHC020000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.235"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(343..1374)
                     /gene="rsmC"
                     /locus_tag="ACHOSM_RS24375"
                     /old_locus_tag="pgaptmp_004875"
     CDS             complement(343..1374)
                     /gene="rsmC"
                     /locus_tag="ACHOSM_RS24375"
                     /old_locus_tag="pgaptmp_004875"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HGU4561189.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1477..1914
                     /gene="holD"
                     /locus_tag="ACHOSM_RS24380"
                     /old_locus_tag="pgaptmp_004876"
     CDS             1477..1914
                     /gene="holD"
                     /locus_tag="ACHOSM_RS24380"
                     /old_locus_tag="pgaptmp_004876"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HGU4561190.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRNDRFTSGLPH"
     gene            1859..2305
                     /gene="rimI"
                     /locus_tag="ACHOSM_RS24385"
                     /old_locus_tag="pgaptmp_004877"
     CDS             1859..2305
                     /gene="rimI"
                     /locus_tag="ACHOSM_RS24385"
                     /old_locus_tag="pgaptmp_004877"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGU4561191.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESFGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2320..2997
                     /gene="yjjG"
                     /locus_tag="ACHOSM_RS24390"
                     /old_locus_tag="pgaptmp_004878"
     CDS             2320..2997
                     /gene="yjjG"
                     /locus_tag="ACHOSM_RS24390"
                     /old_locus_tag="pgaptmp_004878"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HGU4561192.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGIAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
BASE COUNT          785 a          877 c          853 g          755 t
ORIGIN      
        1 ggggacttga acccccacgt ccgtaaggac actaacacct gaagctagcg cgtctaccaa
       61 ttccgccacc ttcgcatacc atcaattctt aaaaagaatt gctaccacgg aggcgcattc
      121 tagtggtttt cagcttttcg tcaatagtta attatcgccg gaggtgtaat tgctggaaaa
      181 atgtccatca ggaaactagc gtgcaggttt ggtaggcatg cgggggcaga tgccagatgc
      241 gacgctggcg cgtcttatct ggcctacgaa gggctaacgt gcaggttttg taggtcggat
      301 aaggcgttca cgccgcatcc gacacggtat tcggcgagat aattaacctt tcttcgcctg
      361 gcgggtcata atggcgcgat acaccttgaa acgcccggtt tgcgcaatca cttcatggaa
      421 gccaaatgtc tcatccagca cgtccgggta aggcaggaag gcgttcgcta caattcgcag
      481 ctcgccgccg ctattaagat gacgcaccgc gccgcgaatc agcgtttgcg ccgcatccag
      541 gctggtttgc atcccatcgt ggaacggcgg gttggagatg atcatatcaa aacgaccttt
      601 cacctcggaa aagacgttgc tggcaaagac ttcaccttca acacagttgg ccgcaagtgt
      661 tgcgcggctg gcttctaccg ctggcgcaga gacatcgcac aaggtgagac gaattttcgg
      721 cgaatggcgc gcaaaggcaa ctgaaagcac ccccgcgcca cagccgacat ccagcacttt
      781 acctttcgtg tgcggagtta acgtcgagag cagcaactgg ctaccgacat ccagaccgtc
      841 gcggctaaac acgccaggca gcgttttgac cgtcaggcca tcgacgctgt attcgcccca
      901 gaatttatcg gcatcaaata ccggctgttt ttccagacga ccaaaataga ggccacagcg
      961 acgagcgctg tcgactttat tcaacggcgc ataatctgcc agcatctgct cggcgctgcg
     1021 cacgccgctg cggttctcgc caacgacaaa aatatctgtc cccactggca gcagagaaag
     1081 taaattcatc aactggaact gggcttccgg tttgttcttc ggccagtagt aaatcagcgt
     1141 atcgcaatct gcgacgtcat ccgccgtggc gaccagactg aaacgggcgt tatcccccat
     1201 ctggcggctt aatacctgcc agtggtggaa ttgctgggta tgagcacggc tggccgcggt
     1261 atctaaacgc gcgggcaggt catcctgtaa gtctccggca aacagaatac ggctttgttc
     1321 gaaatcatca ctgtgacgca gcaagacttc acttgccggg gtaaatgcag acatggaatg
     1381 ctcctcaatt gatactggcg gcgattatag ccatatgttg gcgcggtatc gacgaatttg
     1441 ctatatttgc gcccctgaca acaggagcga ttcgctatga catcccgacg agactggcag
     1501 ttacagcaac tgggcattac ccagtggtcg ctgcgtcgcc ctggcgcgtt gcagggggag
     1561 attgccattg cgatcccggc acacgtccgt ctggtgatgg tggcaaacga tcttcccgcc
     1621 ctgactgatc ctttagtgag cgatgttctg cgcgcattaa ccgtcagccc cgatcaagtg
     1681 ctgcaactga cgccagaaaa aatcgcgatg ctgccgcaag gcagtcgctg caacagctgg
     1741 cggttgggta ctgacgaacc gctatcactg gaaggcgctc aggtggcatc accggcgctc
     1801 accgaattac gggcaaaccc aacggcacgc gccgcgttat ggcaacaaat ttgcacatat
     1861 gaacacgatt tcttccctcg aaacgaccga tttaccagcg gcttaccaca ttgaacaacg
     1921 cgcccacgcc tttccgtgga gtgaaaaaac ctttgccagc aaccagggcg agcgttatct
     1981 caactttcag ttaacgcaaa acggcaaaat ggcggcgttt gcgattacgc aagtggtgct
     2041 ggatgaagct acattgttca atattgcggt cgatcctgac tatcagcgtc agggattggg
     2101 aagggcgctg ctggaacatc tgatcgacga actggaaaaa cgcggcgtgg cgacactatg
     2161 gctggaagtc cgtgcctcaa atgctgccgc cattgccctg tacgaaagtt tcggctttaa
     2221 cgaggcgacg attcgccgca attactaccc caccacggac ggtcgcgaag acgccatcat
     2281 catggcgttg ccaatcagta tgtaagacaa ggtggaataa tgaagtggga ctggattttc
     2341 tttgatgccg atgaaacgct gtttaccttt gactcattca ccggcctgca gcggatgttt
     2401 cttgattaca gcgtcacctt taccgctgaa gattttcagg actatcaggc cgttaacaag
     2461 ccactgtggg tggattatca aaacggcgcg atcacttcat tacagcttca gcacgggcgt
     2521 ttcgagagct gggccgaacg gctgaaagtt gaagcaggct tgcttaacga tgcctttatt
     2581 aatgcgatgg cggaaatctg cacgccgctg ccgggcgcgg tttctctgct taacgccatt
     2641 cgtggcaacg ctaaaatcgg catcatcacc aacggtttta gcgccttgca gcaagtgcgt
     2701 ctggaacgga cgggcctgcg tgattacttt gatttgctgg tgatttccga agaagttggc
     2761 attgccaaac cgaataagaa aattttcgat tatgcgctgg aacaggcggg caatcctgac
     2821 cgttcacgcg tgctgatggt tggcgacact gccgagtccg atattctcgg tggcatcaac
     2881 gccgggctgg cgacctgctg gctgaatgcg caccatcgcg agcaaccaga aggcatcgcg
     2941 cccacctgga ccgtttcatc gttgcacgaa ctggagcagc tcctgtgtaa acactgattg
     3001 cctccccccc gttgatgggt aaaatagccg caatttttcg ttttcaacaa gcgcggcgcg
     3061 atgccgctta ctcaagaaga aagaattatg acgttgtctc cttatttgca agaggtggcg
     3121 aagcgccgca cttttgccat tatttctcac cccaataatt aagcccaaat aaaagctctt
     3181 ttactctttc aaaatccttt cgattaattg agtgataatt atataactca tttaataaca
     3241 tgatgttatg tgatttcttt gattgaaaat
//
LOCUS       DAZKHC020000235         3210 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZKHC020000235 DAZKHC020000000
VERSION     DAZKHC020000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.236"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(31..438)
                     /locus_tag="ACHOSM_RS24395"
                     /old_locus_tag="pgaptmp_004879"
                     /pseudo
     CDS             complement(31..438)
                     /locus_tag="ACHOSM_RS24395"
                     /old_locus_tag="pgaptmp_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(672..1349)
                     /locus_tag="ACHOSM_RS24400"
                     /old_locus_tag="pgaptmp_004880"
     CDS             complement(672..1349)
                     /locus_tag="ACHOSM_RS24400"
                     /old_locus_tag="pgaptmp_004880"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HGU4561193.1"
                     /translation="MWHYLHYKVSRGKPHAECDAKRRAGSPPGPDAHQLQRSQHKKSR
                     QQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYEQRWLIEEYHKAWK
                     SGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGSISEETQNDSCEKILTPTEWKLLWV
                     KLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGWFRHQDMAEGYLVM
                     KSLDQEI"
     gene            1547..3166
                     /locus_tag="ACHOSM_RS24405"
                     /old_locus_tag="pgaptmp_004881"
     CDS             1547..3166
                     /locus_tag="ACHOSM_RS24405"
                     /old_locus_tag="pgaptmp_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc38 family transposase"
                     /protein_id="HGU4561194.1"
                     /translation="MGNSMHSLKTLLQLPCGWRCSRQIISSDGITLHLHGKRKKAQCP
                     ECFKRSDSVHSCRRRRIQHLPCSGHTLWLVFSVRHWYCRNPACSRKIFAGSLAPFAGS
                     HQQSSQALQNLQRQLGLIAGGEAGKRAATAVGLRCSADTLLRRVINTPETKQSGAPHV
                     GIDEWAWHRGHRYGTLIVNLDTHRPLVLLPGRDQRTLATWFRKYPEIQIVSRDRSGVY
                     ATAAREGAPQARQVADRWHLLKNIGDALERMMYRHIPLIRLVASELSPKKSPDPEPSV
                     PAASLRRPERLKQQTRKKRHQRWTEVMALHNKECSFREISRITGLSRVTVSRWVRSGT
                     FPEMSTRPPKRGLLAPWREWLKEQRESSNYNASRIWREMVARGFTGSETIVRDAVAKW
                     RKGWNPPVTTAVRLPSVSRVSRWLMPWRITRDEENYASRFISLMCEKEPELKIAQQLA
                     LEFYRILKTQNKSQLSSWFTRVHESGSAEFRRVAAGMEADAAAICEAISSRWSNGVVE
                     GHVNRLKMLKRQMYGRAGFELLRQRVMSPLA"
BASE COUNT          779 a          820 c          784 g          827 t
ORIGIN      
        1 agccagtttc agattgaaca tattcagcta ttcaaagagc aattcttcat taaactcaat
       61 aaattgttcc ctcagtgttc ggttaaaatg ctcacttatg gcatttattt tcggcgtata
      121 gagataagtc catacgaacc cgacggtggc ttcctgtagc gttttgtcga attttcccga
      181 gaactctttg cccatcatca gtgatgatct ggttgatacc aacaaggaac aactgggcta
      241 cacagcagaa gaaatgcctg atgatatcac ttttaagcga tagtacggcc agagagtagt
      301 tgctgtgttc gtcgagcatg gtaatgaaat aacggtacag ctcgccattc tgagctcaat
      361 agtatccatg ccactgagct tacaggtctt tatcgggcga tagtgcttta ttcttcgggg
      421 ctttgtggat gatttttcga aatgttcatg atagttaggt aaagctaccg catatcattt
      481 gcaaaatgac agatagatat acttcaattt gaatcttaac aattagtatg ggaatgctgt
      541 caatcctctg tatgcgtcca ccacatattg aaatttttgt tctctaatat attccacggg
      601 cgtcaagagt gtgaggcctg tgctgttata cagcaccaga cgttgtccca catctttgac
      661 ctgtctcttg atcagatctc ctgatcaaga gacttcatca ccaggtaacc ctcagccata
      721 tcctgatgtc tgaaccagcc atcccacata actacccaac cggggctgct tgtgcgtttg
      781 ctgtcatgcc atcgccccag tttagccagt ttcaaacagg cccatttcag ggtcggtgtt
      841 tgcgctggaa gctgctttcc ttccagctta acccacagta gtttccactc tgtcggcgtc
      901 agtattttct cacagctgtc attttgtgtt tcttcactga tacttccctg ccgcaggccc
      961 agcacccgca ccgcgataac caccatgcgc tcaaggttat cccgggtctg cattcgcagc
     1021 gattccacac atgtaccacc acttttccac gctttgtggt attcctctat cagccagcgt
     1081 tgctcgtaat ggctgacgat acgtcgcgca tcggcggcac tcgccacttt ttctgatgtc
     1141 agcggatacc agcaggctcc gtcctctcct gctccccgca acagacatac gtgagcgaga
     1201 gcgcctggcc gctgttgtcg ggatttttta tgctgacttc gctgtaactg atgagcatcc
     1261 gggcctggcg ggctgcccgc ccgccttttt gcatcacatt cagcgtgtgg cttcccgcgg
     1321 ctgactttat aatgcaggta atgccagata tccgcttccc ggtcacagac agtgattacc
     1381 tgtttctgta tctcccccag ccgttcggcc atgcgctccg aagcctgctg ccagcggtaa
     1441 ctttcttttt ctgtcacgaa cggtgcaata gtggttcttc cgcaacttgg gtggaagaaa
     1501 atggatgaaa ctttctggtg aacaaagatc taagctgttt taagtcatgg gcaactcaat
     1561 gcactctctt aagacacttc tacagttacc ttgcggatgg cgatgcagtc gacaaattat
     1621 tagctctgac ggtataaccc tccatctcca cggaaaacgc aaaaaagcac aatgtcctga
     1681 atgctttaag cgtagcgact ctgttcatag ttgtcgtcgg cgccggatac agcatctacc
     1741 ctgctcagga catacgctat ggcttgtatt ttccgtccgc cactggtact gccgtaaccc
     1801 ggcttgttca cgtaaaattt ttgccgggtc gcttgctccc ttcgccggtt cacaccaaca
     1861 gtcttcacag gcgttacaaa atttacaacg tcaactggga ttaatcgccg gaggggaggc
     1921 cgggaaacgg gcagcaacgg cagtggggct ccgttgcagt gctgatactc tccttcgcag
     1981 ggttatcaat accccggaga cgaaacagtc aggcgcgcct catgtcggta ttgatgagtg
     2041 ggcgtggcat cggggccacc gttacggtac gttaatcgtc aatcttgata ctcaccgtcc
     2101 cctcgtcctg cttcccggcc gtgatcagcg tacgctggcg acctggttca gaaaatatcc
     2161 ggaaatacaa attgtctcgc gtgatcgcag tggagtctat gcgacagcag cacgtgaagg
     2221 tgcacctcag gccagacagg tggccgatcg atggcatctg ctaaaaaata ttggcgatgc
     2281 gcttgaacga atgatgtaca gacatatacc tctgatacgt cttgttgcca gtgagttgtc
     2341 accaaagaaa tcacctgatc cagaaccatc tgtgcctgca gcatcgctcc gtcgtccgga
     2401 acgcctaaaa caacaaaccc gcaaaaaacg gcatcagcgt tggacagagg ttatggccct
     2461 gcataacaag gaatgtagtt tcagggaaat atcccgtatt acaggcctgt cgcgtgtgac
     2521 agtcagtcgc tgggtgcgtt caggaacatt ccctgaaatg tcaacccgac ctccaaagcg
     2581 agggcttctg gccccatgga gggagtggtt aaaagagcaa cgagaaagta gtaattataa
     2641 cgccagccgg atatggcggg aaatggtggc ccggggattt acaggcagtg aaaccatcgt
     2701 cagggatgcg gttgccaaat ggcgtaaagg ctggaaccca ccggttacta ctgccgtcag
     2761 acttccttca gtgtcccggg taagccgctg gttgatgccc tggagaataa ctagggatga
     2821 agaaaattat gcttcccgat ttattagtct gatgtgtgaa aaagaacctg agctgaaaat
     2881 agcgcagcaa ctggcactcg agttctaccg tattctgaaa acccaaaata aatcacagct
     2941 aagcagttgg ttcactcgag tccacgaaag cggttcagca gaatttcggc gcgtggcggc
     3001 ggggatggaa gctgatgctg cagctatatg tgaggcaatc agtagtcgct ggagtaatgg
     3061 tgttgtcgaa ggtcatgtaa atcgcctgaa gatgctgaaa cgccagatgt atggtcgagc
     3121 aggatttgaa ctactcaggc agagggtaat gagtccactg gcatgaaaat ctccacccag
     3181 gttgcggaag agccactttc cgtcgttgac
//
LOCUS       DAZKHC020000236         3181 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZKHC020000236 DAZKHC020000000
VERSION     DAZKHC020000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.237"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            141..1199
                     /gene="rfaQ"
                     /locus_tag="ACHOSM_RS24410"
                     /old_locus_tag="pgaptmp_004882"
     CDS             141..1199
                     /gene="rfaQ"
                     /locus_tag="ACHOSM_RS24410"
                     /old_locus_tag="pgaptmp_004882"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HGU4561195.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1196..2320
                     /locus_tag="ACHOSM_RS24415"
                     /old_locus_tag="pgaptmp_004883"
     CDS             1196..2320
                     /locus_tag="ACHOSM_RS24415"
                     /old_locus_tag="pgaptmp_004883"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU4561196.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREILRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2313..3110
                     /gene="rfaP"
                     /locus_tag="ACHOSM_RS24420"
                     /old_locus_tag="pgaptmp_004884"
     CDS             2313..3110
                     /gene="rfaP"
                     /locus_tag="ACHOSM_RS24420"
                     /old_locus_tag="pgaptmp_004884"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HGU4561197.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT          830 a          709 c          792 g          850 t
ORIGIN      
        1 cggattgccg cgaaagaagt gatacactag cgcgtcaata tcagtgcggt atcagtattc
       61 aggtagctgt tgagcctggg gcggtagcgt gctttttctg cttcacctta atcggataat
      121 ctcaacaaaa agagttactt gtggataagc catttcgaag aattttgctc attaagatgc
      181 gttttcatgg ggatatgtta ttaactactc ccgtcattag ttcgctgaaa aaaaattacc
      241 ctgacgcaaa aatcgatgtg ctgctttatc aggacaccat cccgatcctg tctgaaaatc
      301 cagagattaa cgcgctctac ggcataaaaa ataaaaaagc aaaagcctca gaaaaaattg
      361 ccaacttttt tcatctcatc aaggtattac gtgccaataa gtatgacctt atcgtcaatc
      421 tcaccgatca atggatggtt gctatactgg ttcgcttatt aaatgcccgt gtgaaaattt
      481 cccaggatta tcatcatcgg cagtctgctt tttggcgtaa aagtttcacc catttggtgc
      541 cgttgcaggg tggaaatgtg gtggaaagta acttatccgt gctgacccca ttgggagttg
      601 attcgttggt gaagcagaca accatgagtt acccgcctgc aagctggaaa cgtatgcgtc
      661 gcgaacttga tcacgctggt gttggacaaa attatgtggt tatccaacct acggcgcggc
      721 aaatcttcaa atgctgggac aacgccaagt tttccgctgt gattgatgcc ttacatgctc
      781 gtggttatga agttgttctg acgtccggcc cagataaaga cgatctggcc tgcgtcaatg
      841 aaattgcgca gggatgccag acgccaccag taacggcgct ggctggaaag gtgaccttcc
      901 cggaacttgg tgcgttaatc gatcatgcgc agctgtttat tggcgttgat tccgcaccgg
      961 cgcatattgc cgctgcagtt aatacgccgc tgatatcgct gtttggtgcg acagaccata
     1021 ttttctggcg tccctggtca aataacatga ttcaattctg ggcgggagat taccgggaaa
     1081 tgccaacgcg cgatcagcgt gaccgaaatg agatgtatct ttcggttatt ccggcggcag
     1141 atgtcattgc tgctgtcgat aaattactgc cctcctccac gacaggtacg tcgttatgat
     1201 cgttgctttt tgtttatata aatattttcc ctttggcggt ttgcagcgcg attttatgcg
     1261 tattgctcag acagtcgccg cccgaggtca tcatgttcgg gtttataccc agtcgtggga
     1321 aggcgaatgc cctgatgtat ttgaactgat caaagtgccg gttaaatcgc ataccaatca
     1381 cgggcgcaat gcggagtatt ttgcctgggt gcaaaaacat ttacgcgaac atcccgtcga
     1441 taaagtcgtt ggattcaaca aaatgccggg gctggacgtt tattatgccg ctgatgtttg
     1501 ttatgccgag aaagtcgcgc aggaaaaagg ctttttctat cacctgacgt cacgttatcg
     1561 ccattatgcc gcctttgagc gggcaacctt cgaacagggc aagccgacac agctgctgat
     1621 gctgacagat aagcaaatcg ccgatttcca gaaacattat cagactgaag cggagcgttt
     1681 tcatattctg ccaccgggga tttatcctga tcgtaaatat agccagcagc cagctaatag
     1741 ccgtgaaatc ttgcgtaaga agaatggaat aaccgaacaa caatatttat tgttgcaggt
     1801 cggttcagac ttcacgcgta aaggtgtcga tcgttccatt gaagcacttg cttcgttacc
     1861 ggattcgctg cgccacaaca cattgctata tgttgttgga caggataaac cgcgaaaatt
     1921 tgaggcgctg gcagaaaaac gcggcgtgcg cagtaatgtt cacttcttct cggggcgcaa
     1981 cgatgtctcg gaattaatgg cggcggcgga tttattactg catcctgcct accaggaagc
     2041 agcgggaatt gtgctactgg aagcgattac tgcaggatta ccggtactaa caacagccgt
     2101 ttgtggctat gcgcattata ttgtcgacgc caattgcggc gaggctattg ctgagccatt
     2161 ccgccaggaa acattgaatg agattttacg caaagcgtta acgcaatctt cattgcgcca
     2221 ggcctgggcg gaaaatgcgc gacattatgc tgatacacaa gatttataca gtctgccaga
     2281 gaaagcggcg gatatcataa cgggtggtct ggatggttga acttaaagag ccgtttgcca
     2341 cgttatggcg cggtaaagat ccttttgagg aagttaaaac cttgcagggt gaggtttttc
     2401 gtgaactgga aactcgccgc actctgcgat ttgaaatggc gggcaaaagc tattttctca
     2461 aatggcatcg cggcacgacc ctgaaagaga taatcaaaaa tttactctca ttgcggatgc
     2521 cagtattagg cgcagaccgc gaatggaatg cgattcatcg actgcgggat gtcggcgttg
     2581 atactatgta tggggtggca tttggcgaaa aaggcatgaa tccgctgacc agaacttcat
     2641 ttattattac cgaagatctg acaccaacca taagtcttga agattactgt gctgactggg
     2701 cgactaaccc tccagatgtt cgcgtaaagc gtatgcttat taagcgtgtc gcgacgatgg
     2761 tgcgcgatat gcatgctgcg ggcattaacc accgtgactg ttatatctgt catttcctgc
     2821 tgcacttgcc tttttccggt aaggaagagg agttaaaaat ttcggtaatt gacctgcacc
     2881 gggcgcagct tcgcacgcgc gttccacgtc gttggcggga caaagatctt attgggcttt
     2941 atttttcttc gatgaatatc ggcctgactc agcgggatat ctggcggttt atgaaagtgt
     3001 attttgccgc cccgcttaaa gacattctca agcaggaaca aggactgctg tcgcaagcag
     3061 aagcaaaagc cacaaaaatc agggaaagaa cgattcgaaa atcgttgtaa cccgtaactt
     3121 atttttgcca aaattttgga tacagaataa atatgtctca actcaatgat agtgacatca
     3181 t
//
LOCUS       DAZKHC020000237         3130 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZKHC020000237 DAZKHC020000000
VERSION     DAZKHC020000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.238"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(299..1777)
                     /gene="ygcE"
                     /locus_tag="ACHOSM_RS24425"
                     /old_locus_tag="pgaptmp_004885"
     CDS             complement(299..1777)
                     /gene="ygcE"
                     /locus_tag="ACHOSM_RS24425"
                     /old_locus_tag="pgaptmp_004885"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HGU4561198.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
     gene            complement(1804..3081)
                     /gene="yqcE"
                     /locus_tag="ACHOSM_RS24430"
                     /old_locus_tag="pgaptmp_004886"
     CDS             complement(1804..3081)
                     /gene="yqcE"
                     /locus_tag="ACHOSM_RS24430"
                     /old_locus_tag="pgaptmp_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HGU4561199.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
BASE COUNT          838 a          796 c          707 g          789 t
ORIGIN      
        1 tacgctgttg atgaatagtt cttatgaata aagatatcca gttcatactt taagtgaaaa
       61 ttgataaagt gcgattcgta ttgtctttta ttctaaagac atcgagtgta gttaatattc
      121 cttgtaaaaa cagggataaa ccgaactagt taaagttttt attatgttcc ctgcatcagc
      181 ggggataaac cggacttgtt atcatttttt aagttgtcga acttatgttc cccgcgtcag
      241 cggggataac cattcaggta tgttcaggcc gggcagtttc ccgcccggcc ttcctcactt
      301 acgcattcga ccagctctgg atcgaatcca cattccccaa ttccccatgc atcacttcat
      361 acgatttttt caggatcaca tcagtatgtt tggttaggtc tttgaaaatg cctttattca
      421 tcgcgtcgta gcgtttggca ttgctctcaa tcggtataaa gatatctttc acgcgaacca
      481 tcttatcaac agccgttgcg taatccgggt atagccccag acctaccgct gtattaatcg
      541 ctgctcccag acttgcacaa ccgttaatgg cgttacggcg tgccggaagg ttgaacacgt
      601 cggcaaaaat ttgcataaac agatcgctgt tcgaaccgcc gccagtaatg atcacatgct
      661 tcgcaaagtg attcatttca ttacacatat tgtcgtaatt gttcttcagc gttagcgcca
      721 cgctttccaa tatcgaacga tatatccatg cgtaatccat gctggaatca aagccgatca
      781 taatcccgcg tttgtacggt tcccacggat tggtcagcca gtccagcacc gtcatcagcc
      841 cattacagcc aggtggcaca ctagaggctt tcttgttgag taaatcttcc ggtgaaagat
      901 cctgcgccct ggcatcctga attaacgact cgcctaacat gtcacgcagc cagctcaccg
      961 tccacatacc tttgcgaata ccgtaacctt catacagcaa tgtttgcgga atagaagaca
     1021 taatcggcca gtacgccacc ggatctttcg gcagtgcttt gccgttcatc atcaatgcga
     1081 tgtaagtgcc aagagaaatt accgcagttt catcatccag taatccggcc cccagagctt
     1141 ctaccggttt atcactggtg gtacaaacaa ccggcagtcc tgccgggaaa tgtgtcgcca
     1201 gtgcggcttg tggtgtgata tgtccgagga cggtgccagg catttgcaca tcaaacagca
     1261 tatgacgggg gatattaaac ttatccatta ccgcagcatc ttcgctccat gcccagctct
     1321 tataatccac cggccactga ccaaaatagt tggcgatatt gtctttaaac tcgccggtta
     1381 agcgatgcgt cagataaccc gaaaaagagg tgacatacgc cacgtcagga ttcgtatgtt
     1441 cgtaagggcg tgtaacgcgt gcatcctgcc agctaatcaa cggcgcagct ggcgtgccgt
     1501 cggctttcaa taacgcacgg cagcaacgga tggagcccag gccgatgcca acaatatctt
     1561 ctttattccc ggcaaactgg ctcatcaaat cgtgacctgc aaaacataat gatgcccata
     1621 aatcgtcgtc aggatgttct gcagtatcgg catcaggcgt gtgcatcggc tgtaataagc
     1681 ctttaccttc gcaaaccaca ttaccttcca gatcgtacat cacgactttt gtgctctgac
     1741 ttccgccatc aatccctatg atgtattttt tcgacatgat ttttctcctg taattttcct
     1801 ggcttacttg ctgctagcca ttgcgggggc gctatcagca gtacgaattt tttggaacag
     1861 caaaaaggta aagacaatca ccatggcaag cgccgccatt cccatcagcc acatattgcg
     1921 gtaggcttct gctgcgggca gggtgtcttg ccagtggccg ataattgggt aaacgaagac
     1981 atccggcagg aatccaatca ccgaacaaat acctaccgtg gtgcccataa tgtaagacgg
     2041 tgttctcgct tcgccagggc aggcccagta cagcccgcgt gaggcgtaac aggtgaatcc
     2101 cagcagtaaa atcaggccaa tccccatcgc gaccgattgc gggttagagt tcgtgacgag
     2161 cagggcagtt aacgccagca ggccgagtac ggaaaggatt tggatcacgc gggtagggga
     2221 tttcacttta ctgtaggtgg tgataatgcc gccaagtggg ccgcacagcg cgcggaagat
     2281 tttgttgatc acaatcccca tgtagctcgc cgccaccagc gacatgccgt acatttcggt
     2341 cagatagttg gtggagtaac tcagaatggc gtagatggtg aagacaccaa aaatcaccat
     2401 gctgcaatac caggtggtgc tgatgcgcaa aacggccagg atgtcgctaa gctggaatga
     2461 ctgtttttct tcgttattgg cactgcgcag gttgttgtta tcgctaacaa aaaaccagca
     2521 cagaatcccc aacaagatgt aaaccacact atagatgata atgaccgttt tcaggctggt
     2581 gctgtcatcc ggtgcaaagc gagaaaagac ccacatggta aacaccgcca gcgacattac
     2641 accgacgccg cgcagccctt ccatccagcc cataattttc ccttgctcgc tatgatcgcc
     2701 aagcaacgag gcggctttaa tcgacaccga ccacagcatt aaaatcgttg ttatcgcaaa
     2761 ggcgacctga atacagagca ttacccacag cggtggatac gttgccatta acagacccag
     2821 taatccggta atgatcatcg cggaagtaat cattttgcgg tgtgaaaatt tatcggcaat
     2881 aacgccgctg ggggcataaa gaataatagc cgcaatacca aaggtactca ttattaaacc
     2941 tatctcggta ttgctgaatc ccataaattt cgccatggga atttgataaa tataacgtaa
     3001 ataagccagg tcgaaactaa cgccgccgct aaaactaatt atcgcgaggg ttatccaacg
     3061 gcgatatgag ttgtgttgca tatcgtattc ccatttgtaa tagacaaaaa aaagagaaaa
     3121 gtaagcctcc
//
LOCUS       DAZKHC020000238         3118 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZKHC020000238 DAZKHC020000000
VERSION     DAZKHC020000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.239"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..179)
                     /locus_tag="ACHOSM_RS24435"
                     /old_locus_tag="pgaptmp_004887"
     CDS             complement(<1..179)
                     /locus_tag="ACHOSM_RS24435"
                     /old_locus_tag="pgaptmp_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HGU4561200.1"
                     /translation="MNEQFRTDLAHTFGITLEEQTNALSFHDHDGHEWILECAPQSEV
                     LFFYCYLFNNEPSKI"
     gene            429..1040
                     /locus_tag="ACHOSM_RS24440"
                     /old_locus_tag="pgaptmp_004888"
     CDS             429..1040
                     /locus_tag="ACHOSM_RS24440"
                     /old_locus_tag="pgaptmp_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HGU4561201.1"
                     /translation="MFSPMTMVGRSLAQAATQTLRPAVTRAATQAGVGASGMKFVPMQ
                     SNFMINHGKLTNQLLQAVAKQTRSGDTQQWFKQEQITYISRTVNRAIDDYCMSNNSVI
                     GKETKSHIFKAVESAIKQPLDMNCAQTSIGHFLQSNKYFNKKVDEQCGKGVDPITRFN
                     TQTRMIEQVSQEIFEQKFGGLKVSDIKALTQSTIAQHVQDTRL"
     gene            1493..3109
                     /gene="tir"
                     /locus_tag="ACHOSM_RS24445"
                     /old_locus_tag="pgaptmp_004889"
     CDS             1493..3109
                     /gene="tir"
                     /locus_tag="ACHOSM_RS24445"
                     /old_locus_tag="pgaptmp_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HGU4561202.1"
                     /translation="MPIGNLGHNPNVRALIPPAPPLPSQTDGAGGARNQLINSNGPME
                     SRLLFTPIRNSVADAADSRASDIPGLPTNPLRFAASEVSLHGALEVLHDKGGLDTLNS
                     AIGSSLFRVETRDDGSHVAIGQKNGLETTVVLSEQEFSSLQSLDPEGKNKFVFTGGRG
                     GAGHAMVTVASDIAEARQRIIDKLEPKDTKETKEPGDPNSGEGKIIEIHTSTSTSSLR
                     ADPKLWLSLGTIAAGLIGMAATGIVQAVALTPEPDDPITTDPDAAANTAEAAAKDQLT
                     KEAFQNPDNQKVNIDENGNAIPSGELKDDVVAQIAEQAKAAGEQARQEAIESNSQAQQ
                     KYDEQHAKREQEMSLSSGVGYGISGALILGGGIGAGVTAALHRKNQPAEQTITTRTVV
                     DNQPTNNASAQGNTDTSGPEESPASRRNSNASLASNGSDTSSTGTVENPYADVGMPRN
                     DSLARISEEPIYDEVAADPNYSVIQHFSGNSPVTGRLVGTPGQGIQSTYALLASSGGL
                     RLGMGGLTGGGESAVSTANAAPTPGPARFV"
BASE COUNT          939 a          599 c          721 g          859 t
ORIGIN      
        1 tcaatttttg aaggttcatt attgaataaa taacaataaa aaaacaggac ctcagattga
       61 ggtgcacact ccagtatcca ttcatgacca tcgtgatcat ggaaagatag ggcgttagtt
      121 tgttcttcaa gagttattcc gaacgtatgt gcaagatctg tgcgaaattg ttcattcata
      181 ttgtagtcct gtcattcttt caatgagggt atgcttaaac gtaattaact cgtgagctct
      241 ggcacaaata ataagacgaa aatataagat gcaagtaata gtgttgcata tttatgatag
      301 catgtgataa atgtagtgca actacttgta gatagtgccg gaggaaaatt taaatataaa
      361 acaattattg tttttaatga aaataaaaac attttgttat cgttatataa tatataaagg
      421 tttattgtat gtttagtcca atgacaatgg taggtagatc gttagctcag gcggctacac
      481 aaactcttag acctgcggtc accagagctg cgacacaagc gggggtggga gccagtggca
      541 tgaagttcgt gcccatgcag tcgaacttta tgattaatca tggcaaactg actaaccagc
      601 tacttcaggc cgtggccaaa caaacccgta gcggtgatac tcagcaatgg ttcaaacagg
      661 agcaaattac ttatatatcc agaacagtaa acagagctat tgatgactat tgcatgagta
      721 ataattcggt aattggtaag gaaaccaaaa gccacatttt caaggctgtt gaaagcgcga
      781 taaagcagcc tctcgatatg aattgtgcac aaacgtcgat tggccatttc ttacagtcca
      841 acaaatactt taataaaaaa gttgatgagc agtgtggcaa aggggtggat ccaattacac
      901 gctttaacac ccagaccagg atgatagaac aagtctcaca agaaatcttt gaacagaaat
      961 ttggtggttt gaaagttagt gatatcaaag ctctgactca gagcacgatt gcacagcatg
     1021 tacaggatac tcggttgtag gaataacctg ttatatatgc gtgttgggca taagttaagc
     1081 ttcctggtgt atagcgtagt ctttgctgat gtaagaaaag gctatatacg gaaacttgat
     1141 atactataaa cttcattatt tattatattg aaaaaagtta tttaattgat ttgatttgtt
     1201 ttgtaatttt aatgcttcaa ttggataagc ataaaatata gttttattta tagtgtgttt
     1261 tattaaagtt tcacgtggca tttattttta tatcgcatga ttattttcat ttaattacac
     1321 tgatgtgtat ttttctgcat tattttcttg catcaaaaat cagactgtga tttatttggt
     1381 cgatgttatg ttcagccgta tatcgactac gtactgtata ccattacatt attatgtaat
     1441 agcaatataa aggattatta tctatttaca taaaataaaa aggagatatt ttatgcctat
     1501 tggtaatctt ggccacaatc ccaatgtgag agctttaatt ccacctgcac cgccattacc
     1561 ttcacaaacc gacggtgcag gaggtgcccg taatcagctc attaactcaa atggcccgat
     1621 ggagtctcgt ttgctattta cgcctataag gaattctgtt gctgatgctg ctgattctcg
     1681 tgccagtgat attcccggac ttcctacaaa tccactgcgc tttgctgcgt ccgaggtatc
     1741 tttgcatggt gcgcttgaag ttcttcatga taaagggggg cttgatactc ttaactctgc
     1801 tattggatct tcgttattcc gtgttgaaac tcgggatgat ggcagccatg ttgctatcgg
     1861 gcaaaaaaat ggcctcgaga ccactgttgt tttaagtgag caagagtttt ctagcttaca
     1921 gtcccttgat cctgaaggta aaaacaaatt tgtatttact ggaggccgcg gtggcgcagg
     1981 gcatgctatg gtcacggttg cttcagatat cgccgaagcc cgtcagagga taatagataa
     2041 attagaacca aaggatacaa aggagacgaa ggagccaggg gatccaaata gtggcgaggg
     2101 aaaaatcatt gaaattcata cctcaacctc aacttctagc ctccgtgcag atcctaaact
     2161 ttggttgtca ttggggacta ttgctgcagg tctgataggg atggctgcga cggggattgt
     2221 acaggctgtt gcgttgactc cagagccgga tgacccaatc actaccgacc ctgatgctgc
     2281 agcaaacaca gctgaagcag cggcaaaaga tcagttaacg aaagaagcat tccagaaccc
     2341 agataaccag aaagttaata tcgatgagaa cggaaatgca attccgtccg gggaactaaa
     2401 agatgatgtt gttgcgcaaa tagcagaaca agctaaagcg gcgggtgaac aggccagaca
     2461 ggaagctatt gaaagtaatt ctcaggcgca gcaaaaatat gatgaacagc atgctaaacg
     2521 cgaacaggaa atgtctcttt catcgggggt tggctacggt attagtggtg cgctgattct
     2581 tggcggggga attggtgccg gtgttactgc tgctcttcat cggaaaaacc aaccggcaga
     2641 acaaacaatc actacacgta cggtagtcga taatcagcct acgaataacg catctgcgca
     2701 gggcaatact gacacaagtg ggccagaaga gtccccggcg agcagacgta attcgaatgc
     2761 cagcctcgca tcgaacgggt ctgacacctc cagcacgggc acggtagaga atccgtatgc
     2821 tgacgttgga atgcccagaa atgattcact ggctcgcatt tcagaggaac ctatttatga
     2881 tgaggtcgct gcagatccta attatagcgt cattcaacat ttttcaggga acagcccagt
     2941 taccggaagg ttagtgggaa ccccagggca aggtatccaa agtacttatg cgcttctggc
     3001 aagcagcggc ggattgcgtt taggtatggg aggattaacg ggggggggcg agagcgcagt
     3061 aagtactgcc aatgccgcac caacgccggg acccgcacgt ttcgtttaaa tatatccg
//
LOCUS       DAZKHC020000239         3098 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZKHC020000239 DAZKHC020000000
VERSION     DAZKHC020000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.240"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            72..>3098
                     /locus_tag="ACHOSM_RS24450"
                     /old_locus_tag="pgaptmp_004890"
     CDS             72..>3098
                     /locus_tag="ACHOSM_RS24450"
                     /old_locus_tag="pgaptmp_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HGU4561203.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRCFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIEWDYANNVIIILPSGIAPSYLEQYSRFELR
                     LRKHLSLTEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGQTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVLPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYSMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRQDARSWLLKYPEHAITGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLMQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDACTADSLAEFVWDLFTAWLTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDASGSRLKDLPKPNKSDDESRSNDA"
BASE COUNT          856 a          792 c          724 g          726 t
ORIGIN      
        1 ttgtctctct ctaacccgca taaatactgg tagcatctgc attcaactgg ataaaattac
       61 agggatgcag aatgagacac tttatctatc aggacgaaaa atcacataaa ttctgggcag
      121 ttgagcaaca gggaaacgag ttgcatatca gttggggaaa agttggcacc aaagggcaaa
      181 gccagataaa aagtttttca gatgctgcgg cagcggcaaa agcggagctt aagctgattg
      241 cggagaaggt gaagaagggg tatgtggagc aagcgaagga taattctttg caaccttccc
      301 aaacggtaac gggctctctc aaggtagcgg acttatccac cattattcag gaacaaccct
      361 ctttcgtagc agaaacccgt gcgcctgaca aaaatacaga tgctgtttta ccgtggctgg
      421 cgaaagatat tgctgtcgtt tttccgcccg aagttgtaca caccacgtta agtcatcgct
      481 gctttcccgg agttcctgtt cagcaagcag acaaattgac ccaattacgt cgcttagcct
      541 gtagtgtgtc gcaacgggat aataaaacag ccacatttga cttcagcgcc tgttctttag
      601 aatggcaaaa caccgtcgcc caggcgatca gtcagatcga cggcctgaaa acaacacagt
      661 taccatcacc agtaatggct gtactcacgg cacttgaaat gaaatgcaca agatataaag
      721 tgcgtgagga tgttatggat cagatcgtcc aggaaggcgg tctggaatat gctactgatg
      781 taataataca ccttcaacag attgatattg aatgggatta tgcgaataat gtcattatta
      841 ttctgccgtc tggcattgca cctagctact tggagcaata ttccagattt gaattacgcc
      901 tacgtaaaca tttatcactg acggaagagt ctctctggca aaaatgtgca caaaaactta
      961 ttgccgcaat tccacatatt ccagaatggc ggcaaccatt aattgctttg ttattacccg
     1021 aaaaaccaga aattgcacat gaaattgccc agcgtctact ggggcaaaaa aaattaccct
     1081 cgcttgagtg gttaaaaata gtggcgactg atgagcacat tcttgcctca ttagaaaaat
     1141 atcacgaacc atatgccatt tttgatgatt actattgtgg tgcgatatgg tcagccaccg
     1201 tattacagga gcaaggtgtt gcagccctgc cccgatttgc tccctatgcc gcaagtgact
     1261 actgcgccga tgtgttgcgt catatcaatc atccgttcgc attgacactg cttatacgtg
     1321 tagccgggca aactaaacgc tgtcacgatc ggatgacgaa agccattgct gcgttcccac
     1381 atgcagcaat ggcggcactg acggaacttc ttgggcaaaa agaagagaac agttggcgca
     1441 ttatgctaat gacaatgctt atctcacaac cagcactggc agaacaggtc ctcccctggc
     1501 tctcgacacc cgcagttgcc gtactgaaat catgccagca acaactgaca cagccctcaa
     1561 accatgccag cgccgatcta ctgccagccg tagtagtctc ccctccctgg ctttcgaaaa
     1621 agaaaaaatc gccgattccg gtgctggatt tagcgccatt aggcattgag ccaatctgtt
     1681 atctgacaga agaaatcagt aatcaacttt tggcgaaata tatctggtat tcaaaacaca
     1741 tcacggttag ccatgaagaa agtactacca acctgttggc aaggatgggt tttcaacgac
     1801 ggatcgctgg tacatatatt aaagctcccg aagcggtagt tgaggcatgg ctaaatgaag
     1861 attattcaac cttactaagt gaatttaagg tgtttcattc acctaccggg cattattggc
     1921 agttggggat tttgacaaca ttgccgctgg agaaagcagt aaaagcatgg aatgccctta
     1981 ccctatctcc acataccgat accgaatact ccatgttaca ttttggactc aaagggttac
     2041 ctgggttagt aaactcactt gcacgctatc cacaagaagc cttgcccatc acgaattact
     2101 tcgcagcgag tgagctggca cctgccgtcg cccgcgcctt caacaaactc aaaaccctac
     2161 gacaagacgc ccgtagctgg ctgctgaaat acccggaaca cgccataacc ggcctgctgc
     2221 ctgcggcgct cggcaaagcc ggtgaagcac aggataacgc ccgcgctgcc ttgcgtatgc
     2281 ttaccgaaaa tggtcatcag ccattaatgc aagaaatcgc ccggcgttat aaccagccgg
     2341 aagtaaccga cgcggtgaac gctctgcttg cgctcgatcc cttagataat cacccgacaa
     2401 aaatccccac tcttccggcc ttttatcagc catcgctctg gacgcgcccg gtattaaaag
     2461 caaacgccca atcactgcca gatagtgccc tcctccacct cggcgaaatg ctccgcttcc
     2521 ctcaggaaga ggctctgtat ccgggattat tgcaggtgaa agatgcctgt accgccgact
     2581 cactggctga atttgtctgg gatctgttta ccgcctggct gactgcgggc gcgccgtcga
     2641 aagagagttg ggcgtttact gcgttaggcg ttctcggtaa cgatgatacc gctcgcaaac
     2701 tgacgccatt aatccgcgcc tggcctggtg aatcccagca taaacgcgcc accgttgggt
     2761 tggatattct cgccgctatc ggtagtgata ttgccctgat gcagcttaac ggcatcgccc
     2821 agaaactgaa attcaaagca ttacaggagc gggcaaaaga aaaaattgcc gacattgccg
     2881 agagccgcga actcacggtg gcggagcttg aagatcggtt agcaccggat ctcggtctgg
     2941 atgataacgg ttcgttgctg ctggatttcg gcccacgcca gttcaccgtc agctttgatg
     3001 aaaccttaaa accgtttgtg cgtgacgctt ccggcagccg cctgaaagac ctgcccaaac
     3061 caaacaaaag cgatgatgaa tcgcggtcga acgatgcg
//
LOCUS       DAZKHC020000240         2960 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZKHC020000240 DAZKHC020000000
VERSION     DAZKHC020000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.241"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            75..350
                     /locus_tag="ACHOSM_RS24455"
                     /old_locus_tag="pgaptmp_004891"
     CDS             75..350
                     /locus_tag="ACHOSM_RS24455"
                     /old_locus_tag="pgaptmp_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HGU4561204.1"
                     /translation="MNLRDYLKEKHITQLQFGKLTGLSQVHVSRVLGGYERFSPEKAL
                     RVAEVTNFEVTPHELRPDIYPNPTDGLPVGCKANTQNTQELIHENQA"
     gene            334..756
                     /locus_tag="ACHOSM_RS24460"
                     /old_locus_tag="pgaptmp_004892"
     CDS             334..756
                     /locus_tag="ACHOSM_RS24460"
                     /old_locus_tag="pgaptmp_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4561205.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVASAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            834..1622
                     /locus_tag="ACHOSM_RS24465"
                     /old_locus_tag="pgaptmp_004893"
     CDS             834..1622
                     /locus_tag="ACHOSM_RS24465"
                     /old_locus_tag="pgaptmp_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561206.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNADCNARGVTKSAIDRITFMAGFADALIQVGWLVENDGGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAGSVTQTDQKALPEEEEEEDINTDLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVQFRQALRKPIRTEQGANGAIRELEKFRQQGFTPEQ
                     VIRHSIANEYQGLFVPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            1629..2369
                     /locus_tag="ACHOSM_RS24470"
                     /old_locus_tag="pgaptmp_004894"
     CDS             1629..2369
                     /locus_tag="ACHOSM_RS24470"
                     /old_locus_tag="pgaptmp_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4561207.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLTEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGYT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASLRSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            2395..>2960
                     /locus_tag="ACHOSM_RS24475"
                     /old_locus_tag="pgaptmp_004895"
     CDS             2395..>2960
                     /locus_tag="ACHOSM_RS24475"
                     /old_locus_tag="pgaptmp_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4561208.1"
                     /translation="METVLHALKAMGKANSVELAARIDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGESRVTEERPVKSETQDMLTEEVAPKVSANMMIEFIAQEGAKTCEEI
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGGFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIV"
BASE COUNT          828 a          640 c          838 g          654 t
ORIGIN      
        1 aagatagtct gactattgat ctttaaaatt agtagggcta atatttgctc gaggcataac
       61 gtagaaggtt ggctatgaac ttaagagact atttaaaaga gaaacatatc acccagctac
      121 agtttgggaa gctaacgggt ttatctcagg tgcatgtaag tcgagtgctg gggggctatg
      181 aaagattcag ccctgaaaaa gcattacgtg ttgctgaagt aacgaatttc gaggttacac
      241 ctcatgaact ccggcctgat atctacccga atccaaccga cggtttacct gttggatgta
      301 aggctaacac acaaaataca caggagttga ttcatgaaaa tcaggcatga gcacatcgaa
      361 tcagtgctgt tagccctggc agctgaaaaa gggcaggcat gggtagccag tgcaattact
      421 gaagaatatc tgcgccaggg aggcggcgaa ttgcccctgg taccaggcaa ggactggaat
      481 aatcagcaga acatctatca ccgttggtta aaaggtgaaa cgaaagcgca aagggaaaaa
      541 attcagaaac tgatccctgc ggttctggca attcttccgc gcgagctgcg tcaccgactc
      601 tgcatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc gttgagtacg
      661 gcaattgatg cgcatgatga tgcagtccag gccgtttacc ggaaagcaca tttcagcggt
      721 ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt gccggattgt
      781 tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa caaatggctt
      841 caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc aggcttgctg
      901 agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc tgggcatggg
      961 cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt acaaaaagtg
     1021 caatagatcg catcactttt atggctggtt ttgctgatgc gttaattcag gttggatggc
     1081 tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac gggaaaagct
     1141 ctaaaaaacg ggcggttaca aacgagcgag ttacaaaaat acgcgaactg aaacgaaaag
     1201 gtaacgctgg cagcgttaca caaacggatc aaaaagcgtt accagaggaa gaggaagagg
     1261 aagatataaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg tctaaaaaat
     1321 tcgagccgga ggctatcgag ctgcctgact ggttgccgga aacactctgg catgagtggg
     1381 ttcagttcag gcaggcattg cgaaaaccga ttcgaacgga gcagggcgct aacggggcga
     1441 tacgggaact ggaaaaattc cgtcagcagg gttttacacc tgagcaggtg attcgacaca
     1501 gcatcgccaa tgaataccag ggcttgttcg taccgaaagg tgttcggcct gagacgttgc
     1561 tccgacaggt taacaccgtc tcgttgccgg acagtgcgat cccgccaggc ttcagggggt
     1621 aacagaccat gaaaaatatt gcgacaggcg gcgttctgga gcgtatccgc agactgaccc
     1681 caccacatgt aaccgcccca ttcagaacgg ttgcggagtg gcgtgagtgg caacttactg
     1741 aaggccagaa acgttgcgag gagatcaacc gtctgaatcg tcagttgcgg gtggaaaaaa
     1801 ttctgaatcg ctctggcatc cagccgttgc accgtaaatg ctcgtttgcg aattaccagg
     1861 tgcagaacga cggccagcga tacgcgttaa gccaggcgaa atccatcgct gatgaactga
     1921 tgaccgggta tacaaatttt gcgttcagcg gaaaacctgg taccgggaag aatcacttag
     1981 cggcagctat cgggaatcgc ctgctgaaag acggtcagac agtgattgtg gttaccgtgg
     2041 ctgatgttat gagcgccctg cacgccagct atgacgacgg gcagtcaggc gaaaaatttt
     2101 tgcgtgaact gtgcgaagtg gacctcctgg ttcttgacga aattggcatc cagcgtgaga
     2161 cgaaaaacga gcaggtggta ctgcaccaga ttgttgaccg ccggacggca tcactgcgca
     2221 gtgtcgggat gctgacaaac ctgaatcatg ccgcaatgag cacgcttctt ggtgagagga
     2281 ttatggaccg gatgaccatg aacggtggtc gttgggtgaa ttttaactgg gagagctggc
     2341 ggtcaaacgt tggacgtcag ggtatgtgag aatttttaac gaggtgaatt ttcgatggaa
     2401 accgtattac atgcactgaa agcgatggga aaagccaatt ctgttgaact ggcggcacga
     2461 attgatatca gccgtgaaga agttctcaac gaactgtggg agctcaaaaa aaatggcgtt
     2521 gttgataaaa cgggtcacac ctggtttctg gctgtcgaag gtgaatcccg ggtaaccgaa
     2581 gagcggccag taaaatctga aacacaggat atgctgaccg aagaggtcgc tccaaaagtt
     2641 agcgctaaca tgatgattga gtttatcgct caggaggggg ctaaaacctg tgaagaaata
     2701 gcgggtaagt tcggtgtcag tactcgcaag gttgcttcca cgctggcggt ggtaaccgca
     2761 acggggcggc tggcacgcgt taatcagaac ggtggatttc gttactgcat gccgggcgat
     2821 aatttaccag cagagccgaa agccgcgctg gtaacggaaa gtgatggtaa ggcctttcct
     2881 cagccagcag gtgctgcgtt accagtccgg gaagccgcaa cacaggaaga aattaaaaca
     2941 gaaactgtgg cggacattgt
//
LOCUS       DAZKHC020000241         2921 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZKHC020000241 DAZKHC020000000
VERSION     DAZKHC020000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.242"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            43..675
                     /gene="yqeH"
                     /locus_tag="ACHOSM_RS24480"
                     /old_locus_tag="pgaptmp_004896"
     CDS             43..675
                     /gene="yqeH"
                     /locus_tag="ACHOSM_RS24480"
                     /old_locus_tag="pgaptmp_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4561209.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            1009..1818
                     /gene="yqeI"
                     /locus_tag="ACHOSM_RS24485"
                     /old_locus_tag="pgaptmp_004897"
     CDS             1009..1818
                     /gene="yqeI"
                     /locus_tag="ACHOSM_RS24485"
                     /old_locus_tag="pgaptmp_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4561210.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGDVKNFNNKILNHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTLSYREVNRD"
     gene            1811..2293
                     /gene="yqeJ"
                     /locus_tag="ACHOSM_RS24490"
                     /old_locus_tag="pgaptmp_004898"
     CDS             1811..2293
                     /gene="yqeJ"
                     /locus_tag="ACHOSM_RS24490"
                     /old_locus_tag="pgaptmp_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HGU4561211.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWVENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAELIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(2326..2406)
                     /locus_tag="ACHOSM_RS24495"
                     /old_locus_tag="pgaptmp_004899"
     CDS             complement(2326..2406)
                     /locus_tag="ACHOSM_RS24495"
                     /old_locus_tag="pgaptmp_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561212.1"
                     /translation="MKDVDQNFDALYWHILQVYLILLFYD"
     gene            complement(2442..2867)
                     /gene="yqeK"
                     /locus_tag="ACHOSM_RS24500"
                     /old_locus_tag="pgaptmp_004900"
     CDS             complement(2442..2867)
                     /gene="yqeK"
                     /locus_tag="ACHOSM_RS24500"
                     /old_locus_tag="pgaptmp_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HGU4561213.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWTLNSRYVNVNSVNKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLILSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
BASE COUNT          993 a          503 c          515 g          910 t
ORIGIN      
        1 aatgaaatta tgcaattata tacggatagg gaggttctta acatggggct ttgtagtcgt
       61 tataaaagtc ttacatgcaa tagttgctca atgcattgcc aaataatgcc agaagagtca
      121 ccgcgtttgc agtactgcgc aaactcgtgt ttttgtatgt ggcccgaaga aagttcatat
      181 tttaatcgtg gtgtggtaga aggtatctta acaaaaaacc acaatgcgag attaagcgga
      241 tatatttttg tcgatttttc agtaagtttt ttacgtctat tccttgaaaa agactggatt
      301 gactatcttg caagtactga catggggatt gttttagtca gcgaccgcaa tatgcaatcg
      361 ttagctaatt actggcgaaa acacaactct gcaatatctg ctgtcatata taatgatgat
      421 ggtcttgatg ttgccaatga aaagatcaga caactattta ttggtcgtta tttatcattt
      481 accagaggta acacattaac tcagatggaa ttcaccataa tggggtatat ggtttctggt
      541 tataatccat atcagattgc tgaagttctg gatatggata tccgtagcat ctacgcgtac
      601 aagcaacgaa tcgaaaagag aatgggtggt aaaataaacg aattatttat tcgttcacat
      661 tcggtccaac attgatactc aaaaatctgc cggaatcata ctctgtttat acgggcattt
      721 attgaaatgc ccgtatttta taaataggcc atcccctcaa gagtaaacat catagtattt
      781 aaaaccatca cacgaaaaat tcagaagcat tacgtaatat gaatttacaa atagtagtgg
      841 cataaatgtt aaccatgtta atttacataa agttttacgt tgcgacatta aagcctcatt
      901 tcaatcatca tgataaatat aaaattaata tatatttatg ccgtaaatac caatatactt
      961 agcaaactat gtgatttcca tttcgattga tttagtgttt attgacgtat gtactggatt
     1021 attaacgata atatcgagtt ctggcctgag caccgaaaat taatatcggt acataacgcc
     1081 gatcttaacg tcgttctgac aacgccagcc agtcgttgtt tatcacttct acttgaagct
     1141 tttcctgatg tggttgcaca acaagatttt ttcaccagag tctgggaaga agaaggtatg
     1201 cgtgtgccta ctaacacgtt atatcagaac atatccatta tcagacgcgg atttcgcgct
     1261 gttggtgata ctacccactc gcttattgca accgtgccga gaagaggatt caagatccat
     1321 aatgacatta acatacaaaa tcatgtaata aactcgtcaa cagacgcaca tacacacaat
     1381 gccccacctg ccataaaagt taatgcggga tacaaagaga gcattggtga cgtaaagaat
     1441 ttcaataaca aaatcctcaa ccatataaaa agttatttaa ttatgttgag cgcatttgtc
     1501 ataggcgcat attctgcata ttggctatgg aataataatc aaccgaagcc atttttcaag
     1561 gattacaaga ctgttgcgga aattaatggc tgccatttta atgtaacaga agatacaatt
     1621 gatgggttga aagagttcga taaatataaa acacggatac tggattcggg gattaactgt
     1681 aaaaaatatc cgtggttata cttccctctt gcaaaatcct caccggggat gattgttatg
     1741 gcatgcaata aaaactataa ccaacatgaa gtggcaggct gtttaaccct gtcttaccgt
     1801 gaggttaatc gtgattgatt ataaaaaaaa cttattattt attttagttt ttatcagtgg
     1861 gtttattctc ttcactgtct attcttacac tgcagaaaaa atgatatata acgaaacatg
     1921 cactgcaaat tgggtaattt ttaatgacca gggacgagca aatcttacca tcgactttat
     1981 gtataacaaa aaaaataaaa caggaactgt tgcgttgagt ggaacctggc aacaaggtaa
     2041 cagagaaagt aaatcaatac ggcgaaatat tgaatacaca tgggttgaaa actatgacac
     2101 agcccattta acatcaaaga aagttaataa gtttgagatt atggatcaag tcgatgatga
     2161 tagacttgca gaacttattc ctgattttta tgtctttcca gaaaaaagtg taagctataa
     2221 tatactaaag caaggtaagc atgcttttat tttgagtatt ggtaacagag caataatgca
     2281 ttgtgcaagg taaactaaaa tagacaacaa aaagatggaa cctcgttaat cataaaacaa
     2341 taaaattaaa tatacttgaa gaatatgcca gtataaagca tcaaagtttt gatcaacatc
     2401 tttcatttta gacatctcct tgcaatgtaa aatatcctgt atcaacaaca tctggtgcaa
     2461 atccatgagt cgaactcgac attcatctta gctcgattat tattccttcg tacagtcgat
     2521 gtaaacaata cagaaagagg attattaaga accagtttac tcagtgaatc aatagaggaa
     2581 aggattaacg tttctttaaa agaattaatt tcatcatctg ttaaactaaa ctcatcattg
     2641 acagatcgtg agatataact gtttttaact ttactcttta cgttgacttt attgacagaa
     2701 ttaacattca catatcttga atttaatgtc catgatgttg tttgcgtgag aaccttctca
     2761 gcattaagga atttttttaa cggaatcctt ggtttctttt tcgagtccga atttacaata
     2821 tcatgcatat agaccatttc atgaatctgc gaaaactcaa tgtccattgt atacctcaca
     2881 tttttaccgt gactcgatgt tactgttcaa taatcacctt c
//
LOCUS       DAZKHC020000242         2904 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZKHC020000242 DAZKHC020000000
VERSION     DAZKHC020000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.243"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            26..2845
                     /gene="eae"
                     /locus_tag="ACHOSM_RS24505"
                     /old_locus_tag="pgaptmp_004901"
     CDS             26..2845
                     /gene="eae"
                     /locus_tag="ACHOSM_RS24505"
                     /old_locus_tag="pgaptmp_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type beta"
                     /protein_id="HGU4561214.1"
                     /translation="MITHGFYARTRHKHKLKKTFIMLSAGLGLFFYVNQNSFANGENY
                     FKLSSDSKLLTQNAAQDRLFYTLKTGETVANISKSQGISLSVIWSLNKHLYSSESEMM
                     KAGPGQQIILPLKKLSVEYSALPVLGSAPVVAAGGVAGHTNKMTKMSPDATKSNTTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGMASSQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSENMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDIISLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHGGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLPNGQVVDQVGVTDFTAD
                     KTSAKADGIEAITYTATVKKNGVAQANVPVTFSIVSGTATLGANSARTDGNGKATVTL
                     KSATPGQVVVSAKTAEMTSPLNASAVIFVDQTKASITEIKADKTTAKADGSDAITYTV
                     RVMKEGAPVVDQKVTFSKDFGTLNKTEATTDQNGYATVKLSSNTPGKAIVSAKVSGVG
                     TEVKATTVEFFAPLSIDGDKVTVIGTGITGALPKNWLQYGQVKLQATGGNGKYTWKSS
                     NTKIASVDNSGVITLNEKGSATITVVSGDNQSATYTINAPGSIVIAVDKNTRVTYFDA
                     ENKCKTNSANLAQSKELLANIYSTWGAANKYPYYSGSKSLTAWIKQSSSEQSSGVSST
                     YDLVTKNQLINVGVNDKNAFSVCVK"
BASE COUNT          850 a          561 c          681 g          812 t
ORIGIN      
        1 aactcattgt ggtggagccc ataacatgat tactcatggt ttttatgccc ggacccggca
       61 caagcataag ctaaaaaaaa catttattat gcttagtgct ggtttaggat tgttttttta
      121 tgtcaaccag aattcatttg caaatggtga aaattatttt aaattgagtt cagattcaaa
      181 actgttaact caaaatgccg ctcaggatcg ccttttttat acgttaaaaa caggtgaaac
      241 tgttgccaat atttctaaat cacagggtat cagtttatcg gtaatttggt cactgaataa
      301 acatttatac agttccgaaa gcgaaatgat gaaggctgga cctggtcagc agatcatttt
      361 gccactcaaa aaactgtctg ttgaatatag tgccttacct gtcttaggtt cggcacctgt
      421 tgttgctgca ggtggtgtcg ctggtcatac gaataaaatg actaaaatgt ccccggacgc
      481 gactaaaagc aacacgaccg atgacaaggc tctaaattat gcggcacaac aggccgcgag
      541 ccttggtagc cagctccagt cgcgctcact gaacggcgat tacgcgaaag ataccgctct
      601 tggtatggcc agcagccagg cttcgtcaca gttgcaggcc tggttacaac attatggaac
      661 ggcagaggtt aatctgcaga gtggtaataa ctttgacggt agttcactgg acttcttatt
      721 accgttctat gattccgaaa acatgctggc atttggtcag gtcggtgcgc gttacattga
      781 ctcccgcttt acggcaaatt taggtgctgg ccagcgtttt ttccttcctg aaaatatgtt
      841 gggctataac gtcttcattg atcaggattt ttctggtgat aatacccgtt taggtattgg
      901 tggcgaatac tggcgagact atttcaaaag tagcgttaac ggctatttcc gcatgagcgg
      961 ctggcatgag tcatacaata agaaagacta tgatgagcgc ccggcaaatg gttttgatat
     1021 ccgctttaat ggctatttac catcatatcc ggcattaggc gccaaactga tgtacgaaca
     1081 gtattatggt gataatgttg ctttgtttaa ttccgataag ttgcagtcga atcctggcgc
     1141 ggcgaccgtt ggtgtaaact acactccgat tcctctggtg acgatgggga tcgattaccg
     1201 tcatggtacg ggtaatgaaa atgatctcct ttactcaatg cagttccgtt atcagtttga
     1261 taaaccgtgg tctcagcaaa tcgagccaca gtatgttaac gagttaagaa cattatcggg
     1321 cagccgttac gatctggttc agcgtaataa caatattatt ctggagtaca aaaagcagga
     1381 tattatttct ctgaatattc cgcatgatat taatggtact gaacacagta cgcagaagat
     1441 tcaattgatc gttaagagca aatacggtct ggatcgtatc gtctgggatg atagcgcatt
     1501 acgcagtcag ggcggtcaga ttcagcatgg cggaagccaa agcgcacaag actaccaggc
     1561 tattttgcct gcttatgtgc aaggcggcag caatatttat aaagtgaccg ctcgcgccta
     1621 tgaccgaaat ggtaatagtt ctaataatgt acagctcact attaccgttt taccgaatgg
     1681 gcaggttgtg gaccaggttg gggtaacgga ctttacggct gataaaacat cggctaaagc
     1741 ggatggcata gaagctatta cctataccgc gacggttaaa aagaatggtg tagctcaggc
     1801 taatgtccct gtaacattta gtattgtatc cgggactgca actcttgggg caaatagtgc
     1861 cagaacggat ggtaacggta aggcgaccgt aacgctgaag tcggctacgc caggacaggt
     1921 cgtcgtgtct gctaaaaccg cggagatgac ttcgccactt aatgccagcg cggttatatt
     1981 tgttgatcaa accaaggcca gtattactga gattaaggct gataaaacaa cagcgaaggc
     2041 agatggttct gatgcgatta cctatactgt cagagtgatg aaggaggggg cacccgtagt
     2101 agatcagaaa gtgacctttt ctaaggattt tgggaccctg aataagactg aagcaacaac
     2161 cgatcagaat ggttatgcta ctgtaaaatt atcatccaat actcctggca aggccattgt
     2221 tagtgcaaaa gtgagtggag taggtacaga agttaaggct actaccgttg agttttttgc
     2281 cccgttgagt attgatggtg ataaagtgac cgtaattggt actggtatca cgggggctct
     2341 gccaaagaac tggttacagt atggtcaggt taagctacag gcaacagggg gcaatggaaa
     2401 atacacatgg aaatccagta atactaaaat tgcttctgtt gataactcgg gagtgataac
     2461 cttaaatgaa aaagggagtg ccacaattac tgtagtatct ggtgataatc agagtgcgac
     2521 atacacaatt aatgcaccgg gtagtattgt aattgctgtg gataaaaata ctcgagttac
     2581 gtattttgat gccgaaaaca aatgtaagac aaatagcgca aatttagcac agtcaaaaga
     2641 actattggcc aatatctatt caacatgggg tgctgcaaat aaatatcctt actattctgg
     2701 ttctaaatca ttgactgctt ggattaaaca atcctcttct gaacagtcat caggtgtatc
     2761 aagcacatat gatttggtta cgaagaacca gttgatcaat gttggagtaa acgataagaa
     2821 tgctttttct gtttgtgtaa aataatttaa ttacatctca aatcccggaa aataaaattc
     2881 attttccggg attttttatt gtac
//
LOCUS       DAZKHC020000243         2877 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZKHC020000243 DAZKHC020000000
VERSION     DAZKHC020000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.244"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(236..1372)
                     /locus_tag="ACHOSM_RS24510"
                     /old_locus_tag="pgaptmp_004902"
     CDS             complement(236..1372)
                     /locus_tag="ACHOSM_RS24510"
                     /old_locus_tag="pgaptmp_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HGU4561215.1"
                     /translation="MYKIRLQKNITILFIFIVMLSFSYWLANSFGMKWGVLAIIGFFL
                     GFTLSFSRFGIVAGWRDMLTMSNSYYVRIHLITIALEVVLFTLCLSFSHAFFGGKMVG
                     NITAISIPFLVGAFVFGIGMQIAGACASGTLYCCGEGRPAFWFVLCCYGIGTLIGNHY
                     KPVLKPIFPGEIITTKDITGGLWSGMLLSLVFILILYLVFSYIEKKRTGKISKIITSG
                     GSFWKDNRFTVLSGGLIIAVLNSLVLAMHGSAWTITGAIYDTSLHTASFLGVLSDNSV
                     ITKPLFQNPMVGMFVGGLLGAAMATVLTGKRIKEPLTRGSIMAGITGGLLMGTGGAYA
                     SCNLGGFFDGTASGSLHGWLWMFMALAGSVIGINLRRFFICSRC"
     gene            complement(2108..2467)
                     /locus_tag="ACHOSM_RS24515"
                     /old_locus_tag="pgaptmp_004903"
     CDS             complement(2108..2467)
                     /locus_tag="ACHOSM_RS24515"
                     /old_locus_tag="pgaptmp_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4561216.1"
                     /translation="MLPNEGRNARQKPPDQKQQSGGMYAPSGQQRPERLKIWGLTRKK
                     KILYGARSTGVKQGEETEVNMDTITDKKAEQLESQGLWRRAAARWLDVMKEAHTDPQR
                     EHIARRREICLANFRML"
     gene            complement(2385..2579)
                     /locus_tag="ACHOSM_RS24520"
                     /old_locus_tag="pgaptmp_004904"
                     /pseudo
     CDS             complement(2385..2579)
                     /locus_tag="ACHOSM_RS24520"
                     /old_locus_tag="pgaptmp_004904"
                     /inference="COORDINATES: protein motif:HMM:NF017845.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
     gene            2664..>2877
                     /locus_tag="ACHOSM_RS24525"
                     /old_locus_tag="pgaptmp_004905"
     CDS             2664..>2877
                     /locus_tag="ACHOSM_RS24525"
                     /old_locus_tag="pgaptmp_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561217.1"
                     /translation="MRLTQYKKEILSYFTPDNLAWVTAEIGSPPFDVSGVAYLLHGMD
                     SFNSRYQIESTRRTLEAMVRDGLLERI"
BASE COUNT          765 a          701 c          551 g          860 t
ORIGIN      
        1 tataccggac ggaggttaaa atacgaaaat ttcgtaggtt gtgagtatat ttcagatagt
       61 cggttgctca gctaattttt ctagagtagg tagtactacg tcgcgtagtt actgtttttt
      121 gatagtacgc tttttcagca tgcatccccc ttgtatacat ttaaaacaag ggggctttac
      181 tttctgataa tagaattctt cccacagttt cctgtcagga ttcaattatt tgtttttaac
      241 agcgtgaaca aataaaaaat ctccgaagat ttatcccaat tacgctgcca gccagagcca
      301 taaacatcca caaccatcca tgtaaacttc ctgatgcagt tccatcgaag aatcctccca
      361 aattacagga agcatacgct cctcctgttc ccataagcaa tccaccagtt attcctgcca
      421 ttatgcttcc acgagtcaga ggctctttga tcctttttcc cgttagtaca gtagccattg
      481 cagcccccag caatccacct acgaacatcc caaccatagg attctgaaat aatggtttag
      541 taataacaga attatcagat aaaaccccaa gaaatgatgc agtatgcaag gatgtatcgt
      601 agatggctcc ggtaattgtc catgctgaac cgtgcattgc cagaaccagt gagttcagta
      661 cagctataat taacccgcca gaaagaacag taaatctgtt atctttccag aaagaaccac
      721 cactggtgat aattttagat atttttccgg tgcgtttttt ctcaatgtat gaaaaaacaa
      781 ggtatagtat caatataaat acaagactta ataacatgcc tgaccacaag ccgccagtaa
      841 tatctttggt ggtaataatt tctccgggaa aaattggttt tagcactggc ttatagtgat
      901 tgccaattaa tgtccctata ccatagcagc acaaaacaaa ccaaaatgcc ggacggccct
      961 caccacaaca atagagtgtt cctgacgcac atgctccggc aatttgcatt ccaataccaa
     1021 aaacaaacgc acccaccaga aaagggatac ttattgctgt aatattccca accatcttcc
     1081 cgccaaaaaa agcatggctg aatgacaggc agagtgtaaa caaaaccacc tcaagagcga
     1141 tggtaattaa gtgaatcctt acatagtatg agttgctcat ggtcagcatg tctctccaac
     1201 ctgcaactat cccaaatcga ctaaaagaca atgtaaaacc caagaaaaaa ccaataattg
     1261 caagtacgcc ccacttcata ccaaaactat tagcaagcca gtaactaaat gacagcatga
     1321 caataaaaat aaataatata gttatgtttt tctgaagcct tattttatac atttttaata
     1381 tacctatatg tttccacgtg atgcgctgat gtacttgctg aaaaggaatc gttccagaaa
     1441 tgtatctacg caatatcctg aacacgttac ccaaaatgcc tgttccagaa tgatactcac
     1501 ctataaataa ccgtcaatat gacgctcaag tactttctgt gagataatgc agcgaattga
     1561 tactgacaat tcaacgtccg tcatcgctcc cccaatgtgt tgaggttagg tagaaatgat
     1621 aacaaataca ccactcctgc ccctgtaacc tccccttttt tatacgctca tttttgcgca
     1681 ctttttcgat catcacgtat cggtttcgtt ttgcgcttcc tgttgcgttt cgtttttgag
     1741 aaaaatgcgc agttttttgc gcactttttg aggtgtctat ccccctgcaa gccgcgtcat
     1801 atctggcttt gctctgtttg tctgtgtgat cgattttgtg atcagtttta tgatcgattt
     1861 gcgcactttt ttgcgcattt tttcagggat ttttcacccc atcgaagagg cttctaaggg
     1921 gctgtgaagt gggtagcatt ttgctgtgat ggaaggacta ccgctactgt ctgataatgg
     1981 tgacggaggc cgctatgttc ggctctgatt gcagcctgcc gtatattcac caatttacct
     2041 ggctgttatt tccataaccc gtaatgtcgc cgatcccgtc tgattcccat atcgtcaaca
     2101 gtcttattta cagcatcctg aagttcgcaa ggcatatttc ccgccgtctg gctatatgct
     2161 ctcgctgcgg atcggtgtgg gcctctttca ttacgtcaag ccaacgagcg gcggctcttc
     2221 gccatagtcc ctgagattcc agttgttcgg cttttttatc agttattgtg tccatattaa
     2281 cttccgtctc ttcgccctgt ttcacccctg tacttcgtgc cccgtaaagg atcttctttt
     2341 ttctggtcaa tccccatatc tttaagcgtt ctggtcgctg ctgccctgac ggcgcataca
     2401 tgcccccact ctgttgtttt tggtctggtg gcttttgccg tgcattgcgc ccttcgttgg
     2461 gtaatatagt gcctctcttc atcagttttt gcctgtctga acaattctcc ccagcgttca
     2521 gcagcacgcc gccagaggcc gctattctcc agcgcttcag ccttgtcatc acgtatcatc
     2581 tgtaccccgc cgattctgtc ctttcaaaat ttgtcttatg gcttattgta tgagggattc
     2641 atcctacaac aagaaaggtc actatgagac tgacgcaata caaaaaagag atcctcagtt
     2701 acttcactcc cgacaatctg gcgtgggtta ctgccgaaat tggcagtccg ccgtttgatg
     2761 tctccggtgt ggcttacctg ctgcatggta tggattcatt caacagccgg tatcagatcg
     2821 aatcaacaag gcgcacgctg gaagctatgg tgcgtgatgg tcttcttgag cggatta
//
LOCUS       DAZKHC020000244         2870 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZKHC020000244 DAZKHC020000000
VERSION     DAZKHC020000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.245"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>2870
                     /locus_tag="ACHOSM_RS24530"
                     /old_locus_tag="pgaptmp_004906"
     CDS             <1..>2870
                     /locus_tag="ACHOSM_RS24530"
                     /old_locus_tag="pgaptmp_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012578873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HGU4561218.1"
                     /translation="NFHIHDATTLPDMKKVQYKQRYQQELVLRGNYASATDQLRVYIL
                     KEYGGIYTDYDVTPAYGKNVYKIIQEHCINYDFLEKEHHRRALNDEILSVISKEPSSG
                     IKNKLPEQDRKRLSVIVDKIKQLDPKDIFSPIDTMIIRDSMVMSKRYQYWGKEKGWNI
                     RANNNFLATHKGSKVTDFVIKRQEIAYKRLFEIREKLRDTGNIAQQHSDNNINSFDFH
                     RGDKKAEAKLVVSGVKHAANKEKNKEVLKDLKKDLATYGALLNNNENGLKPKDIRATE
                     DFLRGYSSGSADRIVDGFRSDMNIKKLVKLMKKNINSLSNEQKGAMAYEIEKRALLAT
                     FHSNVEEYHKLFNNVVSKGSTDKYAVESLIPQLFFLNLSGDGYGGRCDPLSILVLTAK
                     HLENQSSQKQTKLFLENLYSTAAVLSEPSLYSEVEIANANKLLTSLAKLHAKNPLSTT
                     LTQIWKEKIAHQTVEQVIDLIITPKATGNPVLLKLEAPGHAMAAWAIGNDNERKYGFY
                     DANVGVVEFSDTEKFKEYMTEFFSASGLNKAENYHLKKLPPHNSPVFDQIVLLDGEKL
                     SQYKTSYNDDAIKNILDIKVFDNASKQISVSQPIKKDFIPKKRYAENSLFSNYRMDSI
                     IPKKYSTLFISGPDAIMMAMKDYYNSLGELGECRIDREARNFKGLAEDSFVGNLKVIE
                     GAKGVHYDWVNTNSVCVNDVTPDDASTWVAKLEKIEDIFSDVDKLKKNSGWNITPTEI
                     NLSKLTVGWPSEIKKAIKEKWPEMEADYNRIISGSTIDLEEISQVDQKISQHLISQTN
                     SLVKWVGISLAEQLAVKIKHLTLPIENKIHYFMSDIHKHFKDNVQSINSLLASDSNTV
                     VYIWNDDNINKSLILRELLIMEFRRQSLYKLLNGQHDKTKRRFLLEYLRLKEKEQLGI
                     IDKNRQERLLIISTNLSEDDFLKAKLIDIENKALLSSLTMIS"
BASE COUNT         1089 a          421 c          554 g          806 t
ORIGIN      
        1 gtaattttca tattcatgat gcaacaacgt tgccagatat gaaaaaagta caatataaac
       61 aacgatatca acaagaattg gtattgcgtg gcaattatgc gtctgctaca gatcaattac
      121 gtgtatatat actcaaagaa tacggtggaa tatatacaga ttatgacgtc actcctgctt
      181 atggaaaaaa tgtctacaaa ataattcaag agcattgtat taattatgat ttcttagaga
      241 aagagcacca tcgaagggca ttaaatgatg agattctttc tgttataagt aaagagccaa
      301 gctcgggaat aaaaaacaaa ctcccggaac aagatagaaa acgtctctct gtcatcgtcg
      361 acaagataaa acaacttgac cctaaagaca tattttctcc tatcgacaca atgataatca
      421 gagactcaat ggtgatgtca aaacgttatc aatattgggg aaaggagaaa ggctggaata
      481 ttcgtgcaaa taataatttt cttgccacac ataaaggaag taaagtaact gatttcgtaa
      541 taaagagaca agaaatagct tataaaaggc tttttgagat aagagaaaag ttacgagata
      601 caggcaacat agcacaacaa cattcagata ataatattaa ttcttttgat tttcatcgag
      661 gagataaaaa ggcagaagca aagttggtgg tatcgggggt gaaacatgcc gctaacaaag
      721 agaaaaataa agaggtcctt aaagacttaa aaaaagattt ggcgacatat ggagcgctgc
      781 ttaataacaa tgaaaatgga ttaaaaccta aagatattag agcgacagag gatttcttaa
      841 gaggctatag ttcaggaagt gcagatagga tagtggatgg attccgtagt gacatgaaca
      901 ttaaaaagtt agttaagctg atgaaaaaaa atataaatag cttaagtaac gagcaaaaag
      961 gagctatggc ctatgagatt gaaaaacgtg cattactggc tacattccat tctaatgtag
     1021 aagagtatca taagcttttc aataatgtcg tatcaaaagg ttctacggat aaatatgctg
     1081 ttgaatcctt gataccacaa ttatttttct taaacctttc aggtgacgga tatggaggtc
     1141 gttgtgatcc gttgtctatt ttggttctta ctgcaaaaca ccttgaaaac caatcttcgc
     1201 agaagcaaac aaaactattt ctggaaaatc tatattcaac agcagcggta ttaagtgagc
     1261 ctagtttata tagtgaagta gaaatagcaa atgccaacaa actgttaaca agcttggcta
     1321 aacttcatgc aaaaaatccg ttgagcacta ccttaacaca aatatggaaa gaaaaaatag
     1381 cacatcaaac agttgagcaa gttatagatt taataatcac gccaaaggct acaggtaatc
     1441 cagttttatt gaaactggag gctcctggtc atgcaatggc tgcctgggct attggtaacg
     1501 ataatgagcg aaaatatggt ttttatgatg caaatgttgg tgttgttgag ttttcagata
     1561 cagaaaagtt taaggaatat atgacagaat ttttctctgc ttcaggattg aataaagctg
     1621 aaaactatca tttaaaaaag cttccaccac ataattctcc tgtcttcgat cagattgttt
     1681 tattggatgg tgaaaaatta tcgcaatata aaacgagtta taatgatgac gccataaaga
     1741 atatattgga cattaaagtg tttgacaatg caagtaaaca aataagtgtt agccagccaa
     1801 taaaaaaaga cttcattcct aaaaaacgtt atgctgagaa tagtcttttt tctaattacc
     1861 gaatggactc aatcatcccc aagaaataca gcaccctttt tatttctggc ccggatgcca
     1921 ttatgatggc gatgaaagat tactataatt cattaggtga actgggtgag tgccgaattg
     1981 atagagaggc aagaaacttc aaaggactgg cggaggattc atttgtaggg aatcttaaag
     2041 tgattgaagg ggcaaaaggt gttcattatg actgggttaa tactaactcg gtgtgtgtta
     2101 atgatgttac acctgacgat gcaagtacct gggttgctaa actagagaag atagaagata
     2161 tattttctga tgttgataaa ttaaagaaaa attctggatg gaatataaca ccaacagaaa
     2221 taaacttatc aaagttaact gttggttggc cgtcagaaat aaaaaaagca ataaaagaaa
     2281 aatggcctga aatggaggct gactataata gaataattag tggtagtact atagatctgg
     2341 aggagatatc tcaagtagat caaaagataa gccagcattt gatatctcaa actaatagtc
     2401 ttgttaaatg ggttggcata tcattagcag aacaacttgc tgtgaaaata aagcatctta
     2461 cactccctat tgaaaataag atacattatt ttatgtcaga tatccataaa cattttaaag
     2521 ataatgttca gtctataaac tctcttttgg caagtgatag taataccgtt gtatatatct
     2581 ggaacgatga caatataaat aaatctctca tactgaggga gttattgata atggaattca
     2641 gaagacaatc actttataaa ctgctaaatg gacaacatga taaaacgaag agaaggttct
     2701 tattggaata tctaagactg aaagaaaaag aacagcttgg tattattgat aaaaatagac
     2761 aagaaagact gttgattata tcaacaaatc tttctgagga tgatttctta aaggctaaat
     2821 taatcgacat tgaaaacaaa gcattgcttt catcattgac aatgatatca
//
LOCUS       DAZKHC020000245         2840 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZKHC020000245 DAZKHC020000000
VERSION     DAZKHC020000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.246"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..236)
                     /locus_tag="ACHOSM_RS24535"
                     /old_locus_tag="pgaptmp_004907"
     CDS             complement(<1..236)
                     /locus_tag="ACHOSM_RS24535"
                     /old_locus_tag="pgaptmp_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HGU4561219.1"
                     /translation="MSETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIR"
     gene            complement(233..583)
                     /locus_tag="ACHOSM_RS24540"
                     /old_locus_tag="pgaptmp_004908"
     CDS             complement(233..583)
                     /locus_tag="ACHOSM_RS24540"
                     /old_locus_tag="pgaptmp_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU4561220.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREVTAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(593..919)
                     /locus_tag="ACHOSM_RS24545"
                     /old_locus_tag="pgaptmp_004909"
     CDS             complement(593..919)
                     /locus_tag="ACHOSM_RS24545"
                     /old_locus_tag="pgaptmp_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU4561221.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(916..2840)
                     /locus_tag="ACHOSM_RS24550"
                     /old_locus_tag="pgaptmp_004910"
                     /pseudo
     CDS             complement(916..2840)
                     /locus_tag="ACHOSM_RS24550"
                     /old_locus_tag="pgaptmp_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          576 a          960 c          673 g          631 t
ORIGIN      
        1 cggatatgga caccggattc catcccgcca tcgcgggagc gccgggaaag gaccaccaca
       61 ttgcggcgca gtttccccct gcgtaccggt gcccgtgaca ccacttcttc tttcagcaca
      121 ttcgcccccg cacgggttgc ctcacgcagc acccggttat tttccgcacc actcagaagc
      181 tgcaaatcgc ggctgatgtc ctccagcccc gaaaaatcca gcagggtttc gctcattttt
      241 cccctcccag ccgacagaga atttccagac gcccgccggt cgcatccggc acgggcagcc
      301 cgacaacgtt caggatccgg tcacgccatg gaccactcag cacatgaagt cgtgacgctg
      361 ccgtgacttc ccggccggac tgaccgcgca cccagatgcg gatttccgcc tgcgccattt
      421 ccgcaccgga ctgcatccgc tcccggctgc tcctgccccg gatatccgca tgaattttcc
      481 cgcatgacac ccattcttcc gtcatttctc cggcagcgtt acgggttaac accgggttca
      541 gaacacttat catctgtgtc agacgacctg cagatattgc cattccccct cctcataaca
      601 ccgtcggaca acgcaaatcg taaatcagca cggaaacaga aaacggcagc tccccctgaa
      661 gcagttcttc ccgctccgca agatccggat tccggtacag catcccggtc agtcgcatgg
      721 cagccccctt catccgggtt aatgcctcgc ccgggatcag ttcaccgtcc tcacgaatca
      781 ctttatcccg gctgccctga atgtaggcca gcagcacggc ggtagcctga cgaaccttgt
      841 ccatcagcat gtcatcatcc gcgtcatggt cgacacgcag atgtgccttg atctcttcca
      901 gtgtcagtaa tgccgtcatt ttccgcctcc tgcatcccgc ccacgttttg cagccagggt
      961 ccaggctgat gaatgagctt ctccgggttt atcttcggtc atactgttgc agtgccacag
     1021 cgagcccccc cacgtcaccg tatcgccggg gtggtaggtt tcaccggctc tgaacacacc
     1081 gcggtagagc atcaccggca gggaaaatgt tttttccgta cgctggccac tgctctgccg
     1141 gaccaccaca gtgaacgacc gttcgccggt catgctgacg tcaatatcgg ccaccccgtc
     1201 aaccaggcat tcccatcccc gcatcccgtg cgttttttca tacgcccgcc agagtccgcc
     1261 ctggtgtgtg gcatacgtgc cccggggaaa ggatttttga tcgtcaatgg cggggattat
     1321 ttccagtgcc gtggcatcac gcccgtcctg cggagccggc agggccctca ccgcatccag
     1381 aacagccttc tgcagaacat cgggatcata gtcacgacca tcacgcggaa caggaatatg
     1441 gcttaccgcc tctttcacca tctgttcaag catcggacgc acatcatcgg gggtaatact
     1501 tttgccgtcc gccggtaccg gaatattcgc aaccgcatca ttcaccgcct gcttcagtac
     1561 ttccggatcg taatcacgac catcacgcgg aacaggaata tggcttaccg cctccttcac
     1621 catctgttca agcatcggac gcacatcatc gggggtaata cttttgccgt ccgccggtac
     1681 cggaatattc gcaaccgcat cattcaccgc ctgctgcagt acatccggat cataatcacg
     1741 accatcacgc ggtaccggaa tggcccccac agcgtcatcc accatcgcct gcagaaccgg
     1801 acgcacctca tccaccgtca catgcttctg taataccgcc gacagggaag tcagtttctc
     1861 ttcaaacgct tgtgcctgcg aggccatctt cccctcaaat gtgcgctgta aatccgccag
     1921 caccgtggag aattcttcac ccagcgcacg gataatggac agttcacgct ctgtcatttt
     1981 cgcagtatcc ccctgaacat cgccttcacc gcatcacgct ctgtttcact gattgcctta
     2041 ttaccgtcag atgcgccgtc aggcagttgg gctgaaactc ttttcccgga cgacgcaaac
     2101 ggatcctcac gggcatcacg acgggacagc gcctccagac tgtagttctg ctgctgaaga
     2161 tacagtgcat caccgccggc aaggggcggc aggttctcac gtttacgggc ctcattgggc
     2221 gtgagaagcg tatttttcac cgattcaccc agcgttttca tgcgacgttc gctgtccatt
     2281 ctcagcagcg tggtgacgtc aaactccgtg ctctcgtttt cccctgtttc cagcgcctca
     2341 tccagtaaca gctcaatgga ctcaatcagc gtctgcagac actgggaata atactgctgc
     2401 tccagcgcct ccacgttgtc actggaaggc ggctggccaa cgccaatctt gtaggccggg
     2461 acacggaaca ccgaacagac aatttcagcc gtcatcttca gttgttccac cgtctgcgca
     2521 tccaccggtg aaaacgtcgt ggggttatat ttcgccccgt tgctcaggat cgccgttttc
     2581 cccgcatttt cgccggtata cccgctgtcc cagttgctct tcagtttttt cgcattttct
     2641 tccgttatac tgccggggat ctcaatcacc ccggacggcc tgccgccatt tctgaaaaaa
     2701 gacgttgaat ttgcctgaat atgatgcccc tgcgtggccg ccagcccggc agcatacacc
     2761 ggcggcagcc ccacaagcgg atgaaaaaaa cagttaaacc ggtcgtggat cacttccctg
     2821 gcaggcaccg tcaccgcctc
//
LOCUS       DAZKHC020000246         2807 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZKHC020000246 DAZKHC020000000
VERSION     DAZKHC020000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.247"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            561..1334
                     /locus_tag="ACHOSM_RS24555"
                     /old_locus_tag="pgaptmp_004911"
     CDS             561..1334
                     /locus_tag="ACHOSM_RS24555"
                     /old_locus_tag="pgaptmp_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4561222.1"
                     /translation="MTKHMDIHCQTRIFNSINHDTKYKICIFTPKNIKKNNATLYILD
                     GNSANNYISDILPVIDALPNPPVLVTLGYESWNNLSIHRRAYDYTPDGENAIVDNSKP
                     AWIYFTGGGSQSFRELLLTQIMPWVSTIAPNSSRIGIWGHSLGAIFVLDCLKNNSCFN
                     YYYISAPSLLWQNERIIKIIKDDISESKHTKSICLLNGNLSLDYSASLYPEAIKAESV
                     LRNILTEKYSNFSIVQFPELNHQETFSAALWNSIIHFSI"
     gene            complement(1686..2099)
                     /locus_tag="ACHOSM_RS24560"
                     /old_locus_tag="pgaptmp_004912"
     CDS             complement(1686..2099)
                     /locus_tag="ACHOSM_RS24560"
                     /old_locus_tag="pgaptmp_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU4561223.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRQLNIICSECRNSEVMQRVLIFYTGVMME"
     gene            complement(2115..>2807)
                     /locus_tag="ACHOSM_RS24565"
                     /old_locus_tag="pgaptmp_004913"
     CDS             complement(2115..>2807)
                     /locus_tag="ACHOSM_RS24565"
                     /old_locus_tag="pgaptmp_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4561224.1"
                     /translation="REEAVNELWELKRNGVVDKTGHTWFLAGEGESRVTEERPVKSEA
                     QDMLTGEVEQKVTADMMIEFIGQDGAKTCEELAGKFGVSTRKVASTLAVVTATGRLAR
                     VNQNGKFRYCMPGDNLPAEPKAALVTESDGKAFPQPAGAALPVREAATQEEIKTETVA
                     DIVQPLPSFTETQADELIFPSLRRANLALRRAKSDVQKWERVCAALRELNKHRDIVRQ
                     ITDSSRRVVSEK"
BASE COUNT          781 a          670 c          507 g          849 t
ORIGIN      
        1 atttgccgtt catagattga gcctcagatt aatgttaagt gtcttgcagg acgcgtaatg
       61 ttaactgggg cttttctcta tctgccgttg gtgttcatgc ccgaggcaga tagcctcaag
      121 cacccgcagc tattctactt aacttcccat tacctcgcca atatgaaatc ggtcagaaag
      181 acagacgtcc cataaggaca acaacggaaa aagagatttt gcttgatgct atttacctct
      241 gcaccatgaa gttgcgccaa aatcccagtg gtgataacag gaatttggtt gttggtgatt
      301 ggggaaagaa gtacgttaga ctgtgggtta gttcattgac atgatagtta cctgccttca
      361 atttcctaag caagcgatgt aaacgataat caatatcaaa tgtattgaca ggtaaaataa
      421 taacgatgta cctttgctga taacatatat cagtcttaaa tcataatgga ggtagataaa
      481 ataactaaac acacacaaca aattttttat cagtgacata gcatatctta agcatccttt
      541 aatgctcatg gggagaacgc atgactaaac atatggacat tcattgtcaa acacgcatat
      601 ttaactcaat taaccatgac accaaatata aaatatgtat ttttactcca aaaaacatta
      661 aaaaaaataa tgcaactctt tatatacttg atgggaatag tgccaacaat tacatttctg
      721 atattctgcc tgttattgat gcactaccca atccaccagt gttagttaca cttggttatg
      781 aatcttggaa taaccttagc attcatcgcc gtgcttacga ttatactcct gatggtgaaa
      841 atgctattgt tgataactct aaaccagcat ggatctactt tactggtgga ggaagccagt
      901 ctttccgaga attattacta actcagatca tgccatgggt tagtactatt gctccgaaca
      961 gttccagaat cggtatatgg gggcactcgc taggtgctat ttttgtgctt gattgcttaa
     1021 aaaataattc atgctttaac tattattata tatctgcacc atcacttcta tggcagaatg
     1081 aaagaatcat caagataatt aaagatgata tatcagaatc aaagcacact aaaagtatat
     1141 gcttgcttaa cggaaatctt agccttgatt actccgcatc tttatatcct gaagccatca
     1201 aagcggaatc tgttttgaga aatattttga cagaaaaata cagtaatttt tctattgtac
     1261 aatttccaga gctcaatcat caggagactt tctctgctgc actctggaat agcattattc
     1321 atttcagcat ataacagaaa atatgtaatc actttatcaa tctagctaac ataactgata
     1381 tcaatcctaa tgggtctttg aacatattaa aagataactt ctcatttgta cattgttgtg
     1441 ttcttagacc catttcaaaa atactatttt ttagatacta ataattacat ccatttagct
     1501 atcttctaaa tactcctctc agtttcttca ttggagctga ttcaactccg gcaatgcata
     1561 ctaggatcat tttataagca tacaaacaac atccagactg tgaattattg ctattcattt
     1621 tctatgtatc tgctcttttc ccatccactg aaacaccaga atccaaaggt ttcatgttat
     1681 tttcactatt ccatcattac tcctgtgtaa aatatcaaaa ctcgctgcat cacttcactg
     1741 ttacggcact cactacaaat tatgttcaat tgcctgtcat agcggcgtat ttctccgtct
     1801 ggtaatgacc agataaggtc aggatcaacc acaaccggtt tcttcagttt tgccctcgat
     1861 agttttttgc gggcgttttg ccagtcctta cgagcctgtt cagatgggaa taacccgtaa
     1921 ccagagttgt atacatcgcc actggcaacc agctctctgg caagaacgct catcagatat
     1981 cttgtcgcac ctgtcttggc ttccagttgc cgtaacgtct cgcgcccact ccggcgtact
     2041 agctcaacaa cctgcccttt aattttttcc cgctcttcct gtgtaaatac ttttgccata
     2101 agcgcctccg gcaatcactt ttccgataca acacggcggg aagaatcagt aatctgtcga
     2161 acaatatccc ggtgcttgtt cagctcccgc agcgcggcgc agactcgctc ccacttctga
     2221 acatcacttt tcgccctgcg cagcgccagg tttgccctgc gaagggacgg aaaaatcagc
     2281 tcatctgctt gcgtttcggt aaacgatggc aacggctgca caatgtccgc cacagtttct
     2341 gttttaattt cttcctgtgt tgcggcttcc cggactggta acgcagcacc tgctggctga
     2401 ggaaaggcct taccatcact ttccgttacc agcgcggctt tcggctctgc tggtaaatta
     2461 tcgcccggca tgcagtaacg aaatttaccg ttctgattaa cgcgtgccag ccgccccgtt
     2521 gcggttacca ccgccagcgt ggaagcaacc ttgcgagtac tgacaccgaa cttacccgcc
     2581 agttcctcac acgttttagc cccatcctga ccgataaact caatcatcat gtctgcggta
     2641 actttttgtt cgacctcccc ggtcagcata tcctgtgctt cagattttac tggccgctct
     2701 tcggttaccc gggattcacc ttcgccagcc agaaaccagg tgtgaccagt tttatcaacg
     2761 acgccatttc ttttgagttc ccacagctcg ttgacagcct cttcacg
//
LOCUS       DAZKHC020000247         2800 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZKHC020000247 DAZKHC020000000
VERSION     DAZKHC020000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.248"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            67..396
                     /locus_tag="ACHOSM_RS24570"
                     /old_locus_tag="pgaptmp_004914"
     CDS             67..396
                     /locus_tag="ACHOSM_RS24570"
                     /old_locus_tag="pgaptmp_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4561225.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            383..763
                     /gene="tnpB"
                     /locus_tag="ACHOSM_RS24575"
                     /old_locus_tag="pgaptmp_004915"
     CDS             383..763
                     /gene="tnpB"
                     /locus_tag="ACHOSM_RS24575"
                     /old_locus_tag="pgaptmp_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4561226.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVCGVDWQQV
                     KGHDLAKWVYQEEPEDAQSNAITQ"
     gene            806..2347
                     /locus_tag="ACHOSM_RS24580"
                     /old_locus_tag="pgaptmp_004916"
     CDS             806..2347
                     /locus_tag="ACHOSM_RS24580"
                     /old_locus_tag="pgaptmp_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HGU4561227.1"
                     /translation="MDISLLSTTSDPEQLRALAIAMVQKVMAENAELQNRIRILEEQM
                     KLARQQRFGKKCESLAGMQRSLFEEDVDADIAEISAHLDKLLPQTGDEEKTTTRPVRK
                     PLPSPLPRAEKVIPPAEERCPDCDAPLHFIRDEVSEKLEYIPAQVVVNRYIRPQYSCP
                     CSEKVFSGKMPAHILPKSAVEPSVIAQVVISKYTDHLPLYRQQHIFSRMGVELPVSTM
                     ADMVGVAGAALAPLAKLLRHELLTRDVIHADETSLRLLDTRKGGKSCSGWLWAYVSGE
                     RSGPPVVCFDSQTGRALRYPEAWLQGWHGGTLVSDGYNVYKSLADNHPGITSACCWSH
                     ARRGFANLYKASREPRAAMALRKIAGLYRIEKLIRERPVEKIRQWRQRYSRPIVNDLF
                     AWLEEQEPCCPPEGPLNKAINYILNRRDELSCFLGDGAVPLDNNICERAIRPVVMGRK
                     AWLFAGSLMAGNRAAQIMSLLETAKRNGLEPHAWLTDVLTRLPEWPEDRLEELLPLEG
                     FTFSG"
     gene            complement(2482..>2800)
                     /locus_tag="ACHOSM_RS24585"
                     /old_locus_tag="pgaptmp_004917"
     CDS             complement(2482..>2800)
                     /locus_tag="ACHOSM_RS24585"
                     /old_locus_tag="pgaptmp_004917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561228.1"
                     /translation="TNDRSLTMVDLFCCAGATARLVSEAGSEELRYCSGELILTHFSG
                     EPLAAHTIDHRLTMVDLFCCTGATARLVSEAGSEELSRVIASGREVLTRVLMAGDEVQ
                     PRE"
BASE COUNT          669 a          779 c          754 g          598 t
ORIGIN      
        1 cttcacaggg atcttcagtc agttagcatt accgacatcc attcaccaac ttaccggaga
       61 taatcaatgt caaaaccccg atggacgctt gatcagaaaa aacatcacgt tgccgcatgg
      121 cgcgccagcg gccttacccg tgaacagtac tgtgaacttt acgacattcc gtttaaatcc
      181 ctgcgccagt ggccgcagga tgtcgcaaaa gccgaaaaac gagccagagc accagaaatt
      241 attcctgttt cagtatctgg ctcttccgga atgactgatg gcagaccatt gtcggatgag
      301 cctgtcactc tcttcctgcc cggtggtatc cggatgtgct gccagccttc tcagttaacc
      361 gatgttttcc gggctcttcg ccatgctgac gcctgataac gtattcctgg tcgtaaaacc
      421 cgtcgatatg cgccggggta tcgacacact gacacagtac gtccagaacg aacttaacgc
      481 tgcatggcat gacggtgctg cttttgtctt caccaataaa gtccgctccc gcataaaagt
      541 tctccggtgg gacaaacatg gcgtatggct gtgtacccga cgactgcacc ggggaagctt
      601 ccgctggccc cggaaaggtg atgccacatg gcatctcaca caggatgagt ttcactggct
      661 ggtctgtggg gttgactggc aacaggtgaa agggcatgac ctggcaaaat gggtatacca
      721 ggaagaaccc gaagatgctc agagcaacgc aataactcaa tgaaatataa tgaatatcca
      781 tgcgtgatgt catataatcc accgcatgga tatctcgctt ctcagtacca ccagtgaccc
      841 tgaacagctt cgcgctctgg caattgccat ggtgcaaaaa gtcatggctg agaatgcaga
      901 acttcagaac cgtatccgca tcctggaaga gcagatgaag ctcgcacgcc agcagcgttt
      961 cggtaaaaaa tgcgaatcgc tggccgggat gcagcgttcc ctgttcgaag aggatgttga
     1021 cgcggatatc gcagaaatct cggcgcatct ggacaaactg cttcctcaga ccggggacga
     1081 agaaaaaaca acaacacgtc ctgttcgtaa gcccttgcct tcacctcttc cccgtgctga
     1141 aaaggttatc ccgccagctg aggaacgttg tcctgactgt gatgcccccc tgcacttcat
     1201 tcgtgatgaa gtcagcgaga agctggaata tattccggca caggtcgtgg tcaaccgcta
     1261 tatccgcccg cagtacagct gtccctgcag tgagaaagtg ttcagcggaa aaatgccggc
     1321 acatatcctt ccgaaaagtg ctgttgagcc atcggtcatc gcacaggttg tcatcagcaa
     1381 atataccgac catctgcccc tgtatcgcca gcagcacatt ttctcccgaa tgggggtgga
     1441 gctccccgtc agtacaatgg cggatatggt cggtgttgct ggcgctgcac tggccccgct
     1501 ggcaaaattg ttacgccacg agcttctgac acgggatgtt attcatgcag atgaaacgtc
     1561 cctgaggctc cttgataccc ggaaaggcgg taaatcctgc tccggctggc tgtgggctta
     1621 cgtcagcgga gaaagaagcg gtccgccggt cgtctgtttt gactcgcaga ccgggcgggc
     1681 cctcagatat cctgaggcct ggttgcaggg ctggcatggc ggtacgctgg tgagtgacgg
     1741 gtacaacgtg tacaaatctc tggcggataa tcacccaggt atcacatctg cctgttgctg
     1801 gagccacgca aggcgcggct tcgcaaatct gtacaaggcc agcagggagc cccgtgcagc
     1861 catggcgctg agaaaaatcg ccggccttta ccgcattgag aagctcatcc gtgagcgtcc
     1921 tgtggaaaaa atacggcaat ggcgacaacg gtattcccgg ccgatagtga acgacctgtt
     1981 tgcctggctt gaagagcagg agccgtgttg tccgcctgag ggcccattaa ataaggcgat
     2041 taactatatt ctgaaccggc gcgatgaact gagttgcttc ctgggggacg gagctgtgcc
     2101 cctggacaac aatatctgcg agcgtgccat ccgcccggtg gtcatggggc ggaaagcatg
     2161 gttattcgcc ggttcactga tggccgggaa ccgcgcggca cagataatga gccttctgga
     2221 aacggcaaaa cgtaatggtc tggagccgca tgcctggctg acagacgttc tgacgcgtct
     2281 accggagtgg ccggaagacc gactggaaga gttgctgccc cttgagggat ttaccttctc
     2341 cgggtgagtg atacctgcca tcaggtgttc gtgcagcggg ccataacccg tagtcgggag
     2401 ttgaacgcct gacgacagga aatgagccag agcagcgacc acgcgggctg acagtgagtc
     2461 agaacctgaa tcagcccgga gctattcccg gggctgtact tcatcacctg ccatcagtac
     2521 ccgtgtcagt acttcgcgac ctgacgcaat tacccgactc agttcttcag aacccgcctc
     2581 cgataccagc ctggcggtcg cccctgtgca acaaaataaa tcaaccatcg tcagacggtg
     2641 atcaatcgta tgcgctgcca gtggctcgcc tgaaaaatgc gtcaatatga gttcgccgga
     2701 acaatacctc agttcttcag aacccgcctc cgataccagc ctggcggtcg ccccggcgca
     2761 acagaataaa tcaaccatcg tcagactgcg atcattcgta
//
LOCUS       DAZKHC020000248         2795 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZKHC020000248 DAZKHC020000000
VERSION     DAZKHC020000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.249"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            13..771
                     /gene="paa"
                     /locus_tag="ACHOSM_RS24590"
                     /old_locus_tag="pgaptmp_004918"
     CDS             13..771
                     /gene="paa"
                     /locus_tag="ACHOSM_RS24590"
                     /old_locus_tag="pgaptmp_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HGU4561229.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAKKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGIIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPEADVWITWADWAASNPGIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            1091..1164
                     /locus_tag="ACHOSM_RS24595"
                     /old_locus_tag="pgaptmp_004919"
     tRNA            1091..1164
                     /locus_tag="ACHOSM_RS24595"
                     /old_locus_tag="pgaptmp_004919"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1125..1127,aa:Arg,seq:tct)
     gene            1341..1769
                     /locus_tag="ACHOSM_RS24600"
                     /old_locus_tag="pgaptmp_004920"
                     /pseudo
     CDS             1341..1769
                     /locus_tag="ACHOSM_RS24600"
                     /old_locus_tag="pgaptmp_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310292.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
     gene            1766..1933
                     /locus_tag="ACHOSM_RS24605"
                     /old_locus_tag="pgaptmp_004921"
     CDS             1766..1933
                     /locus_tag="ACHOSM_RS24605"
                     /old_locus_tag="pgaptmp_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU4561230.1"
                     /translation="MTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVLVCGIMVLLW
                     PVIKRNSLHNA"
     gene            complement(2128..2271)
                     /locus_tag="ACHOSM_RS24610"
                     /old_locus_tag="pgaptmp_004922"
                     /pseudo
     CDS             complement(2128..2271)
                     /locus_tag="ACHOSM_RS24610"
                     /old_locus_tag="pgaptmp_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2248..>2795
                     /locus_tag="ACHOSM_RS24615"
                     /old_locus_tag="pgaptmp_004923"
     CDS             2248..>2795
                     /locus_tag="ACHOSM_RS24615"
                     /old_locus_tag="pgaptmp_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4561231.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPSSEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGA"
BASE COUNT          699 a          636 c          769 g          691 t
ORIGIN      
        1 aaggagtttt ttatgaggaa cataatggct ggttttttaa tattcctgtc ttctgctgct
       61 tatgctgata tcaatctgta tggtcctggt ggcccgcata cagccttgct tgatgcagcc
      121 aaactttacg ccaaaaaaac aggtattata gtgaatgttc attacggccc acagaacaaa
      181 tggaatgaag atgccaaaaa aaatgcagat atcttgtttg gcgcatcaga acaatctgct
      241 ctggctatca ttcgggacca taaagacagc ttcagtgaaa aagatattca gcctctttat
      301 ctgcgaaaaa gtattttact ggtaaagaaa ggtaatccta aaaatatccg gagtattgac
      361 gacctgacca gacccgggat tggcataatt gttaatgatg gtggtggtac cagtaataca
      421 tcaggcactg gcgtctggga agatattgcc ggacgtaaag ggaatataga aactgtcgcc
      481 gcaatccgaa aaaatattat tttatatgcg cccaatagcg gaactgcacg taaggctctt
      541 gagaatcagc ctgaagcaga tgtctggata acctgggctg actgggcagc cagtaatcca
      601 ggaattggtg atgtcgtgga aatagcgcca gactatgtga tatggcgtga tatgaacatt
      661 acagtacgtc aggatgcaaa tgatgaaacc cgtcgatttg cagaatggct acaaaccgat
      721 gaagcggcgc ctgcattcaa aaaatatggc tggaccagga aaggcacttg acatccacgt
      781 ccttcaggac gtggattctt tttccggatg ccgcgccagc ggcatgtagg ggcagctcac
      841 aaaacggaaa aaattgtacg ctaagcctcg ccaggtgaac tgaattcatt ccgatatggg
      901 aattcccata tcgggcgaaa acggtttgct gtaacggcag agttaagtag gattgctgcg
      961 ggtgcttgag gctatctgcc tcgggcatga acaccaacgg cagatagata aaagccccac
     1021 cctgtactga accctttaag ttgagtgtgc gaggttcgag tccccggtgg tggtccatta
     1081 tcggtattct gcgttgttag ctcagcagga cagagcaatt gccttctaag caatcggtca
     1141 ctggttcgac tccagtacaa cgcgctacac ttattttccc ggctcgcttt tgcgggcctt
     1201 ttttgtatct gcgccacgcc cggcgcatac caaccacaga gcctttcggg ggggagctta
     1261 cggagtggtc agtgagactt tctctgtggg cagatcgctc ccgggcgttg gctcacccac
     1321 ccaaaggaaa gtcacgatgt ttggtatttt tggtaaaaaa agcccgcaga gcggcaacgg
     1381 aaattaaaaa gtttgaaaaa cgcgatctgg cacaggtggt gattaacgcc gcatacctgg
     1441 tggcctgtgc agatggtgaa tgtgaggctt cctagaaagc gaagatcgaa caggtactgc
     1501 gtaatcagcc tgcgctgtac gcgtttacgt cagaaattaa tgcgattagc gcaaccatta
     1561 tcggtcagct ggatacgaac tttaaaactg gtcgtcgtgc ggcgttacgt gagatcgagg
     1621 atgtgaaaca cgatacgcgt gaagcggaag atgtgctgga tgtggcggtg gccattgcgg
     1681 aggcagacgg cgaaattgag ccggaagagc gcaaggtgct ggaagagatt gccggtgttc
     1741 tgggtcttcg tctggagaat cacctgtgac ggtaaaactg cgcctgactg tggctgcact
     1801 cctgctgttt ctggtggtga tggtggattt caccagcaga atcatgtcgg tgctggcgga
     1861 tggggtgctg gtctgcggca ttatggtatt gctgtggccg gtgataaaaa gaaacagcct
     1921 gcataatgct tgattttttt gtttgctgtt tattaaaaac acttctgcat ggtgaatccc
     1981 cctgtgcggt ggggcaatca gcaagaagga atatggggta atcgcggatt caggtgctga
     2041 tactgaattc accgggaggc acccggcacc atgctttgcc acaaaagtgt tatttctgtt
     2101 tttctcaaac tatcatcgtt atccctttat tttcggctgc gcatggcgcg gccttttttt
     2161 tacgaccagc cactggcaga tggtcatcct gtgatttgat tccgcttccg gctttttaac
     2221 tctgttcctc tacacgggag aaattcgatg tcgattaaac attatgatgt tgtcagggcg
     2281 gcgtcgccgt cagaccttgc ggaaaagctg acacacaaac tgaaagaggg ctggcagcca
     2341 tacggcggac cggttgccat tacgccgtac acactgatgc aggcggtggc tattgaagga
     2401 gagccacagg tcggcccttc atctgagccg gattggtact acgtcatcgt actggccggg
     2461 cagtccaatg ccatggctta cggtgaaggg cttccactgc cggattcata cgatgctccg
     2521 gatccgcgca ttaaacagct ggcgcgccgc agtacagtga cgccgggcgg ggctgcctgc
     2581 agatataacg atattattcc ggccgaccac tgcctgcatg atgtgcagga tatgagtacg
     2641 ctgaatcatc cgaaggcaga cctgagcaaa gggcagtacg gctgtgtcgg ccagggctta
     2701 catattgcca aaaaactgct cccgtatatc ccgaataacg cggggatcct gctggtacca
     2761 tgctgtcgtg gtggttcggc attcacccag ggcgc
//
LOCUS       DAZKHC020000249         2729 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZKHC020000249 DAZKHC020000000
VERSION     DAZKHC020000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.250"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            179..367
                     /locus_tag="ACHOSM_RS24620"
                     /old_locus_tag="pgaptmp_004924"
     CDS             179..367
                     /locus_tag="ACHOSM_RS24620"
                     /old_locus_tag="pgaptmp_004924"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561232.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            695..889
                     /locus_tag="ACHOSM_RS24625"
                     /old_locus_tag="pgaptmp_004925"
     CDS             695..889
                     /locus_tag="ACHOSM_RS24625"
                     /old_locus_tag="pgaptmp_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561233.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1670..2006)
                     /locus_tag="ACHOSM_RS24630"
                     /old_locus_tag="pgaptmp_004926"
                     /pseudo
     CDS             complement(1670..2006)
                     /locus_tag="ACHOSM_RS24630"
                     /old_locus_tag="pgaptmp_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2646..>2729)
                     /locus_tag="ACHOSM_RS24635"
                     /old_locus_tag="pgaptmp_004927"
     CDS             complement(2646..>2729)
                     /locus_tag="ACHOSM_RS24635"
                     /old_locus_tag="pgaptmp_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561234.1"
                     /translation="LQGKVVWLWILPFVIEWLIVCTLGVNS"
BASE COUNT          893 a          579 c          540 g          717 t
ORIGIN      
        1 ggtcaaaggt ttatgtgaaa aatacctgtg ttttctttga aaacacacaa cagtatcagg
       61 ttatttatgt ttatacagtg cgtttcaaac tgacctgaat aattacgcaa gttgcaacaa
      121 taaaacattt ttgccgtagt gtgaggtact ccggtaaatt gctttccagg tgcagacaat
      181 gactaacgaa gaactacagg caatcgcatt atccatgctt gagcgccagc gcgaaaaagt
      241 acaccagcaa cgagagtctt tgcttaaccc atctccattc accaggccaa aacgattgga
      301 gcttgaagca ttcttggaca aatacccacg gcggcttaaa tcaggcaaaa ccagaccacc
      361 aggctgatag ctcacagttt gttctccgat tcaataaaac ggataagaac catcagcgcg
      421 gtagcgtaag gcactacagc gcattgcttt acgagtaata acaatggatt tatcaccaga
      481 agacgcaaag tttttacgag aactgcaaca ccgtatgctc agagaagcca ttatcgaaca
      541 gcaaacgggc atgagcgcca tagacccatt gcgattcaaa cgggcctcat ctcctttagt
      601 tgacgacaat tccaatgcgg ataaaacacg tccaccacac tgattacatt tttcattagc
      661 acagtggggt gatgcagcat acgaggaaaa ccggatgaat gagaacaaaa taaaaaggct
      721 ggagcaactg ttacaggcac gccaacggga gtttgccact aaatggggac gtgccgcgct
      781 gccacatgaa cgcctaacct attttccact tgaagacctc attaaaaata atggcaaaaa
      841 aatcaggtct gataagaaac acttcttaga atgcaatgaa gacaaataac aacaatctgc
      901 atattgccac caaccacaga tgtggcatcg ttatcagcgc acttgatttt ctttatatcc
      961 actgacgagt ttgccggtca ttctggtatc atgcagatat ttacggcgta caagcccact
     1021 agcatgacat tgtaataagg cctgagccta acactttaat gtgtgtcata cactcagtgg
     1081 cgtaatcgtt gacaatatgg tgcatgtaat cgtaagcgtc cagcgaaatc catctggtca
     1141 taatctgaag catccgacaa agtggtgtcc accaaataag tgatctgcgc ccaatatcct
     1201 gagccggaac tttgacaaag gccaacgaaa aatgggagcg gatgttacag agttctcggt
     1261 acaggggaaa agtggtacct gtcgccagtt ctccgttctg tttaaccggg aaattatctc
     1321 ctacagcttg tcagaaaggt cggtgatgga gcatcgttaa tacgatgcta tacgatgcat
     1381 tcacagtgcc cggccagagg atgccccgtc gctgcatatg gatcagggtt ggcaatatcg
     1441 aatcgcaggc tataggcaaa gttaaagccc catggagtag cacaaaatat gccgcacaaa
     1501 ggaaacggtc tgaataacgc agtgatgaag aacttcttca gcacactgct aaaacgcata
     1561 gtgatcgagc gctgattctg gcgaacaact gaactaatac atcagaatct gcattatgtt
     1621 aaataaatat gaaaagatgg tttaaatacc ccgttacttt tgacttacac tatacggtat
     1681 cgcatcgttt aatattcgca ccggccagat ttttatttct attagttgtc acaatactga
     1741 atgcgtacga ccacagtatt ctggctcctg tgtggttatg ctttaattct gcgttccggg
     1801 cagataagca gttgcttgca ggaatccttc ttgtgttaat gtcagttccc ctttttacca
     1861 gtgctgattt ccacattccg tccaacagag cttatagcct ttccctggat tatagcattg
     1921 tccggctgaa gttctttttg aataataata gaagcactgc tggcagatcc agtccgtttt
     1981 tcataaccca ctgtactgat aaccataatc taatcagtag aaattgagtc gaaaataagc
     2041 actactccat acaggataat tagaggtcag tttgattatt cacaattcat catcagcatt
     2101 ttctatttct gacgaaatca atatgaaaat aaccatatat gataattatt ataataacgg
     2161 ctttaattgg aatacatata ttacaacgta ttatatataa ttggtattct gggaactata
     2221 ttctcaaaat acagtagaaa cgaggtatgt ttctggtaga aaggacagtg ggattaaaaa
     2281 gtaagaattg ataaaaaaaa cgccagcaac acatagtgcc ggcggaggga ataccccatg
     2341 gagaaaatgt gatgcctaga gcatcatgaa catgaatata taattaaaaa tagttagcgt
     2401 tgtcaatact ttcacaaaaa taattttcgt agtatagaaa gatattttta tgcatgacct
     2461 acctgaattt gctccgggta gaggttataa ataaaaattg aatcacgaca aacacaatat
     2521 tcatagtatg gcgatgccta cgccagcaag aatagcgcca ataatattgg gaatataata
     2581 gacaccagac gcacaggcat ctcactcctt aacaaacaac aatcaggata tttactttta
     2641 ccaagctaac tgtttacacc taaagtgcac acaattaacc attcaataac aaatggcaat
     2701 atccatagcc atacgacttt accttgtaa
//
LOCUS       DAZKHC020000250         2713 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZKHC020000250 DAZKHC020000000
VERSION     DAZKHC020000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.251"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>2713
                     /locus_tag="ACHOSM_RS24640"
                     /old_locus_tag="pgaptmp_004928"
     rRNA            <1..>2713
                     /locus_tag="ACHOSM_RS24640"
                     /old_locus_tag="pgaptmp_004928"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          716 a          596 c          849 g          552 t
ORIGIN      
        1 tgctaatctg cgataagcgt cggtaaggtg atatgaaccg ttataaccgg cgatttccga
       61 atggggaaac ccagtgtgtt tcgacacact atcattaact gaatccatag gttaatgagg
      121 cgaaccgggg gaactgaaac atctaagtac cccgaggaaa agaaatcaac cgagattccc
      181 ccagtagcgg cgagcgaacg gggagcagcc cagagcctga atcagtgtgt gtgttagtgg
      241 aagcgtctgg aaaggcgtgc gatacagggt gacagccccg tacacaaaaa tgcacatgct
      301 gtgagctcga tgagtagggc gggacacgtg gtatcctgtc tgaatatggg gggaccatcc
      361 tccaaggcta aatactcctg actgaccgat agtgaaccag taccgtgagg gaaaggcgaa
      421 aagaaccccg gcgaggggag tgaaaaagaa cctgaaaccg tgtacgtaca agcagtggga
      481 gcacgcttag gcgtgtgact gcgtaccttt tgtataatgg gtcagcgact tatattctgt
      541 agcaaggtta accgaatagg ggagccgaag ggaaaccgag tcttaactgg gcgttaagtt
      601 gcagggtata gacccgaaac ccggtgatct agccatgggc aggttgaagg ttgggtaaca
      661 ctaactggag gaccgaaccg actaatgttg aaaaattagc ggatgacttg tggctggggg
      721 tgaaaggcca atcaaaccgg gagatagctg gttctccccg aaagctattt aggtagcgcc
      781 tcgtgaattc atctccgggg gtagagcact gtttcggcaa gggggtcatc ccgacttacc
      841 aacccgatgc aaactgcgaa taccggagaa tgttatcacg ggagacacac ggcgggtgct
      901 aacgtccgtc gtgaagaggg aaacaaccca gaccgccagc taaggtccca aagtcatggt
      961 taagtgggaa acgatgtggg aaggcccaga cagccaggat gttggcttag aagcagccat
     1021 catttaaaga aagcgtaata gctcactggt cgagtcggcc tgcgcggaag atgtaacggg
     1081 gctaaaccat gcaccgaagc tgcggcagcg acgcttatgc gttgttgggt aggggagcgt
     1141 tctgtaagcc tgcgaaggtg tgctgtgagg catgctggag gtatcagaag tgcgaatgct
     1201 gacataagta acgataaagc gggtgaaaag cccgctcgcc ggaagaccaa gggttcctgt
     1261 ccaacgttaa tcggggcagg gtgagtcgac ccctaaggcg aggccgaaag gcgtagtcga
     1321 tgggaaacag gttaatattc ctgtacttgg tgttactgcg aaggggggac ggagaaggct
     1381 atgttggccg ggcgacggtt gtcccggttt aagcgtgtag gctggttttc caggcaaatc
     1441 cggaaaatca aggctgaggc gtgatgacga ggcactacgg tgctgaagca acaaatgccc
     1501 tgcttccagg aaaagcctct aagcatcagg taacatcaaa tcgtacccca aaccgacaca
     1561 ggtggtcagg tagagaatac caaggcgctt gagagaactc gggtgaagga actaggcaaa
     1621 atggtgccgt aacttcggga gaaggcacgc tgatatgtag gtgaagtccc tcgcggatgg
     1681 agctgaaatc agtcgaagat accagctggc tgcaactgtt tattaaaaac acagcactgt
     1741 gcaaacacga aagtggacgt atacggtgtg acgcctgccc ggtgccggaa ggttaattga
     1801 tggggtcagc gcaagcgaag ctcttgatcg aagccccggt aaacggcggc cgtaactata
     1861 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacctgcacg aatggcgtaa
     1921 tgatggccag gctgtctcca cccgagactc agtgaaattg aactcgctgt gaagatgcag
     1981 tgtacccgcg gcaagacgga aagaccccgt gaacctttac tatagcttga cactgaacat
     2041 tgagccttga tgtgtaggat aggtgggagg ctttgaagtg tggacgccag tctgcatgga
     2101 gccgaccttg aaataccacc ctttaatgtt tgatgttcta acgttgaccc gtaatccggg
     2161 ttgcggacag tgtctggtgg gtagtttgac tggggcggtc tcctcctaaa gagtaacgga
     2221 ggagcacgaa ggttggctaa tcctggtcgg acatcaggag gttagtgcaa tggcataagc
     2281 cagcttgact gcgagcgtga cggcgcgagc aggtgcgaaa gcaggtcata gtgatccggt
     2341 ggttctgaat ggaagggcca tcgctcaacg gataaaaggt actccgggga taacaggctg
     2401 ataccgccca agagttcata tcgacggcgg tgtttggcac ctcgatgtcg gctcatcaca
     2461 tcctggggct gaagtaggtc ccaagggtat ggctgttcgc catttaaagt ggtacgcgag
     2521 ctgggtttag aacgtcgtga gacagttcgg tccctatctg ccgtgggcgc tggagaactg
     2581 aggggggctg ctcctagtac gagaggaccg gagtggacgc atcactggtg ttcgggttgt
     2641 catgccaatg gcactgcccg gtagctaaat gcggaagaga taagtgctga aagcatctaa
     2701 gcacgaaact tgc
//
LOCUS       DAZKHC020000251         2666 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZKHC020000251 DAZKHC020000000
VERSION     DAZKHC020000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.252"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            44..199
                     /gene="hokD"
                     /locus_tag="ACHOSM_RS24645"
                     /old_locus_tag="pgaptmp_004929"
     CDS             44..199
                     /gene="hokD"
                     /locus_tag="ACHOSM_RS24645"
                     /old_locus_tag="pgaptmp_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU4561235.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            420..677
                     /locus_tag="ACHOSM_RS24650"
                     /old_locus_tag="pgaptmp_004930"
     CDS             420..677
                     /locus_tag="ACHOSM_RS24650"
                     /old_locus_tag="pgaptmp_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561236.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            747..1025
                     /locus_tag="ACHOSM_RS24655"
                     /old_locus_tag="pgaptmp_004931"
     CDS             747..1025
                     /locus_tag="ACHOSM_RS24655"
                     /old_locus_tag="pgaptmp_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561237.1"
                     /translation="MAQNSRLHNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            1027..2076
                     /locus_tag="ACHOSM_RS24660"
                     /old_locus_tag="pgaptmp_004932"
     CDS             1027..2076
                     /locus_tag="ACHOSM_RS24660"
                     /old_locus_tag="pgaptmp_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4561238.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRA
                     LPSGVVPAVHQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWLHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLREQTSDSLDQLAQQNLAAWMIDIIRHAMNGAQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIIPGEQTATSILKQR
                     TKNIALPPHTHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            2089..2463
                     /locus_tag="ACHOSM_RS24665"
                     /old_locus_tag="pgaptmp_004933"
     CDS             2089..2463
                     /locus_tag="ACHOSM_RS24665"
                     /old_locus_tag="pgaptmp_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4561239.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGK
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            2460..>2666
                     /locus_tag="ACHOSM_RS24670"
                     /old_locus_tag="pgaptmp_004934"
     CDS             2460..>2666
                     /locus_tag="ACHOSM_RS24670"
                     /old_locus_tag="pgaptmp_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4561240.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATEL"
BASE COUNT          671 a          650 c          731 g          614 t
ORIGIN      
        1 taggttagcc tcttacgtgc cgaaaggcaa ggagaagcag gctatgaagc agcaaaaggc
       61 gatgctaatc gccctgatcg tcatctgttt aaccgtcata gtgacggcac tggtaacgag
      121 gaaagacctc tgcgaggtac gaatccgaac cggccagacg gaggtcgctg tcttcacagc
      181 ttacgaacct gaggagtaag agaccaggcg ggggagaaat ccctcgccac ctctgatgtg
      241 tcaggcatcc tcaacgcacc cgcacttaac ccgcttcggc gggtttttgt ttttattttc
      301 aacgcgtttg aagttccgga cggcgccgga atagaatcaa aaatacttaa gtagcgcgca
      361 gggagaagag ggatggaccc cgaacagggg agtgctattt atctggaagg attctgttga
      421 tgagaatcga agaattacgt gaaattttta gtgaagatgg cctctatact gtgcgcgttg
      481 agaagggcgc tattgtcagc cactgccgta ttaaatgttt acagtctcaa caaaggaaga
      541 gtggagctgc gttaattcat tttgtggatg ggcttgtgac ggatggtttt attttgcgtg
      601 caaatgaatt tgtcacatcg ttgccgtctc tgaaagaagc tgggattaag gctggttttt
      661 ctgcttttga agattagtga attcatctac aattcagcgc agggctgaac ccctgttgag
      721 taacactgtg ccaccggaga aagccgatgg cgcaaaattc cagactacac aattctgatg
      781 attctgccgt ctttgccagc aggcgcggac ggtgttttca cgcattcaaa tctgactggt
      841 accagcatcc cccatgcact gaagaacagg ccgaatggct cattcagtgt taccgcaggc
      901 gcggatgcga ggttaaaaaa gcccttagcc tcgactaccg tcactggata atctccgtca
      961 ggctccctta ctccgaacgg ccaccgcgtc cgtcccgcac attccagcaa cggatctgga
     1021 ggtaatgtgc gggtattact tcgacctgtt ctggtaccgg aactcggtct ggttatcgtt
     1081 aagccaggcc gtgaatcaat gtcagcattc cataacggca gaatactggt ggagccggaa
     1141 ccaaaaagca tgcgagctct gccgtccggg gttgtacctg ccgttcacca gccgctggcg
     1201 gaagataaat cactactgcc atttttcagc gatgagcggg tgatccgtgc tgcgggtggc
     1261 gctggtgcac tgtctgactg gttattacgt cacgtgaaat cctgccagtg gctacacggt
     1321 gattatcatc acagcgaaac cgtcattcac cgttacggta ccggcgcgat ggtgttgtgc
     1381 tggcactgcg acaaccagct gcgggagcag acatctgatt cactggatca acttgctcaa
     1441 cagaatctgg ccgcctggat gattgacatc atccgtcacg caatgaatgg cgcacaggag
     1501 cgtgaattat ctctggctga attatcctgg tgggcggcct gcaatcaggt ggtggatgca
     1561 ctacctgagg cagtagcgcg tcgttctctg ggattaccgg cggaaaaaat ccgctccgta
     1621 taccgtgaaa gcgacatcat accgggagaa cagaccgcca ccagcatact gaagcagcgc
     1681 acaaaaaata ttgcgctacc gcctcacacc caccagcaac agaacccacc acaggaaaag
     1741 acggtggtca gcattgccgt tgatccggag tctccggaat ccttcatgaa acgacctaaa
     1801 cgtcgccgct gggtaaatga gaaatacaca cgctgggtaa agacacagcc gtgtgcgtgt
     1861 tgtggtaagc cagccgacga tccgcatcac ctgattggtc atggtcaggg cggaatgggg
     1921 acaaaatctc acgatatttt cacgctaccg ctgtgtcggg agcatcacaa cgagcttcat
     1981 gcggatccgc tggcgttcga agaaaagcat ggttctcagg ttgatttaat ttttcgtttt
     2041 cttgatcacg cctttgcaac tggcgtgctc gggtaaaaga ggtgactgat gctcatagat
     2101 ttggttttac cttacccgcc gacggtgaac acttactggc gacgccgtgg cagcacatat
     2161 tttgtatcaa aagccgggaa gcgttatcgc cgggcagtgg cgcttattgt tcgccagcag
     2221 cgactgaaat taagcctgtc cggacggctg gcaataaaaa ttattgcaga gccaccggat
     2281 aagcgccgcc gcgacctgga caatatcctg aaagcaccgc tggatgcgct gacgcacgcg
     2341 gggttgttaa tggacgatga gcagtttgat gaaatcaata ttgtacgtgg aaagccagta
     2401 tctggtggac gtctgggggt gaagatttac cccataatgc ttgaagggca ggtcaaaaaa
     2461 tgaaactgga agatttaccg aaatactact ccccaaaatc ccctggcctg accgatgcat
     2521 cggcctcaac gtcaaaagat gcgctgagta tcactgatgt gatggccgcg cagggcatga
     2581 cacagaatcg ggctgagatg gggttttctg cgttccttgg gaaaatgggc attagtatga
     2641 atgacagaga gcgggcaaca gaattg
//
LOCUS       DAZKHC020000252         2664 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZKHC020000252 DAZKHC020000000
VERSION     DAZKHC020000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.253"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..202)
                     /locus_tag="ACHOSM_RS24675"
                     /old_locus_tag="pgaptmp_004935"
     CDS             complement(<1..202)
                     /locus_tag="ACHOSM_RS24675"
                     /old_locus_tag="pgaptmp_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase N-terminal
                     domain-containing protein"
                     /protein_id="HGU4561241.1"
                     /translation="MTAKNINAGAPTDIVTHWHNINWAKCRREVRRLQARIVKAIQEE
                     KAGKVKALQWLLTHSFSARAMAV"
     gene            824..1435
                     /locus_tag="ACHOSM_RS24680"
                     /old_locus_tag="pgaptmp_004936"
                     /pseudo
     CDS             824..1435
                     /locus_tag="ACHOSM_RS24680"
                     /old_locus_tag="pgaptmp_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            1436..1654
                     /locus_tag="ACHOSM_RS24685"
                     /old_locus_tag="pgaptmp_004937"
     CDS             1436..1654
                     /locus_tag="ACHOSM_RS24685"
                     /old_locus_tag="pgaptmp_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561242.1"
                     /translation="MNYAGHEKLRAEVAEVASAMCDLRTTMNEMEPRYSDADTLPERL
                     VRQTLFRASYLFMKAYNEILELDYCFKD"
     gene            1614..2103
                     /locus_tag="ACHOSM_RS24690"
                     /old_locus_tag="pgaptmp_004938"
                     /pseudo
     CDS             1614..2103
                     /locus_tag="ACHOSM_RS24690"
                     /old_locus_tag="pgaptmp_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            2149..>2664
                     /locus_tag="ACHOSM_RS24695"
                     /old_locus_tag="pgaptmp_004939"
     CDS             2149..>2664
                     /locus_tag="ACHOSM_RS24695"
                     /old_locus_tag="pgaptmp_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561243.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     FFPYAGEWLTEDEIRAVLDAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCW"
BASE COUNT          556 a          773 c          753 g          582 t
ORIGIN      
        1 taactgccat tgctctggcg ctgaacgagt gggtgagtag ccattgcaag gcttttacct
       61 tgcccgcttt ctcctcctgt attgccttca cgatacgcgc ctgcagcctt ctgacctcgc
      121 gacggcactt ggcccagttg atattgtgcc agtgagtcac gatgtcggtg ggtgcacccg
      181 cgtttatgtt tttcgcagtc atctgcttac cctccttcaa aggtattcag gttatctcgt
      241 gttgaaagac catgcggaag tctgcccgct ttcgcgtgga atgatgttgc tgaaacagct
      301 caactcctgt ccatcccatt acaggatggc attcgctttc tccgcactcc tgtacccgca
      361 tcactgacag ctttcctctc ggttagcctg ccctgagggc agtgatacgg gcttaccgtg
      421 ttccgcacag tttgcaggga tgggttaggt tctgcctatc caccggtagc tttgatgtcc
      481 gtgcctcccc agtcaaaaga ggaaggtcca gctacgttcc cattttgggt cgggcctgtc
      541 agtgcctttg gcccgttcag ggttgacgat gtttatcagc agttcacatg tgttaaccct
      601 gccatccagc cttgcgcccc atccgccctg acactggcag acatgcttcc tcctcgcgga
      661 ttccacatgg tgtgaaaacc gggctacgtt gtcccgaagg cttcataccg aacagttacc
      721 cgctctgcat ggttcggtag gctacaggcg acggaacgcc tggttttact acttccttta
      781 catgaagtta aaacaacaaa ctgtgcgact ttcacgtcgc acactgactg acccgccgta
      841 cctcgtcggt ttccgtgacc gttccgggcg caccattgcc ggtgacgtta atgatgactg
      901 gctgcaacct gccagcaatg agatgtaccg cgtactcaaa aaagacgcgc tgatggtcag
      961 cttctacggc tggcaccgta ttgaccgctt tatggcggcc tggatacgcg ccggattcag
     1021 cgtggcaggg cacctcgtgt tcaccaaaaa ctacacatcc aaatctgcgt atgtcgcgta
     1081 ccgccacgaa tgcgcttaca tcctggcaaa gggcagtccg gcccggcccc gtaacccgct
     1141 gccggatgtg ctgggctgga aatattctgg caaccgccat cacccgacgg aaaagcccgt
     1201 taccagcctg caaccgctga ttgagagctt cacatacccg aactcaattg tgctggaccc
     1261 gtttgcaggc agcggttcaa cctgcgtggc cgcccttcag tccggacgcc ggtatatcgg
     1321 tattgagctg cttgagcagt atcaccgtgc cgggcagcaa cgccttgccg ccgtgcaacg
     1381 gaccatgcag cagggggccg cgaatgatga ctggtttatg ccggaggctg cgtaaatgaa
     1441 ctatgccgga cacgaaaaac ttcgcgccga agtggcggag gtggccagtg ccatgtgcga
     1501 cctgcgtaca accatgaatg agatggagcc gcggtacagc gatgcggaca ccctcccgga
     1561 acgtctggtg cgccagacac tctttcgtgc cagttacctg tttatgaagg catataacga
     1621 aattctggaa ctggattact gcttcaaaga ttgaggggag agaagagtat gtacggaaca
     1681 tgcgaaacgc tatgccgggc gctggcagca aagtatccgg gagacacgcc gttgatgtta
     1741 gttatctggt ccccggaaga gattcaggcc ctcgctgacg gaatggatat ttccctgtcc
     1801 gatcatgaaa tcaggaccgt cctggcgcgc ctggaggaca tcccggaaga ccagcggatt
     1861 gagtccggta tttcttccgg cgtggcgatg gagatcatca ataacgtgag agaaaaccgc
     1921 caggtgaccg tcccggctga actgctggcg tccctgattc agaccgccga acaggcactg
     1981 tggaaacgtg aatgggccgc ccgtgattac ggcctcgccg tcccggaatg cgttacccgc
     2041 cgtcaggcgg tggttaatca ggcccgcatc ctgctgaaaa acaacacaca cgaaaacgac
     2101 tgatgttatc gccgcctccg ggcggcgatt cagggagaac gattaatcat gaacgaaaac
     2161 acaacgctga acgcactgat ttgtcgtcac gcccgcaacc tgctgctggc gcagggctgg
     2221 ccggaagaga cggatgttga ccagcggaac ccgaagtatc cgggctggat cagcatttat
     2281 gttctgctgg atgcgccccg gctggcgacg ttactcatca accgtcacgg cggcgtactg
     2341 ccgccgctcc tggcctccgc cattcagaaa ctgaccggaa ccggggcgga actggtactg
     2401 tccggcagtc agtggcagtc gctgccggta cttccggcag acggaacaca ggtgtttttc
     2461 ccgtatgccg gagaatggct gaccgaagac gaaatcaggg cagttcttga tgcagtgcgc
     2521 gatgcggtac gcagtgtcag ttgccgggtg gcagaagatg cgcggcgtat cagggcggcg
     2581 ctgaccacaa ccggtcagac gttgctgacc cgccagacgc gccgctttcg cctggttgtg
     2641 aaggaaagcg atcacccctg ctgg
//
LOCUS       DAZKHC020000253         2655 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZKHC020000253 DAZKHC020000000
VERSION     DAZKHC020000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.254"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1107)
                     /locus_tag="ACHOSM_RS24700"
                     /old_locus_tag="pgaptmp_004940"
     CDS             complement(<1..1107)
                     /locus_tag="ACHOSM_RS24700"
                     /old_locus_tag="pgaptmp_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4561244.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESYPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNGREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIP"
     gene            complement(1201..1392)
                     /gene="ydfD"
                     /locus_tag="ACHOSM_RS24705"
                     /old_locus_tag="pgaptmp_004941"
     CDS             complement(1201..1392)
                     /gene="ydfD"
                     /locus_tag="ACHOSM_RS24705"
                     /old_locus_tag="pgaptmp_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HGU4561245.1"
                     /translation="MNSAFALVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            complement(1389..1577)
                     /gene="dicB"
                     /locus_tag="ACHOSM_RS24710"
                     /old_locus_tag="pgaptmp_004942"
                     /gene_synonym="ftsT"
     CDS             complement(1389..1577)
                     /gene="dicB"
                     /locus_tag="ACHOSM_RS24710"
                     /old_locus_tag="pgaptmp_004942"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4561246.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            complement(1588..2442)
                     /locus_tag="ACHOSM_RS24715"
                     /old_locus_tag="pgaptmp_004943"
     CDS             complement(1588..2442)
                     /locus_tag="ACHOSM_RS24715"
                     /old_locus_tag="pgaptmp_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4561247.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKAGAGRGNPMMTTAHNRAHAVFLC
                     VMHSHIQIMVGRAGQPKGWPVSVVTGISTPVRLTTLMVVESLGGELFKLTIEAAIMAT
                     IHTLSHPDVTIENGRAVTTSIAIAEFFGKRHERVLDKIRNLDCSAKFTEHNFVSSEYT
                     DSTGRKLPMYQITKNGFVFLVMGFTSKKAAAFKEAYIAEFDRMEKELRQNNAPSPDKM
                     IHGDGRTLVIRLDEHGNIKFTETVPDGAMVCTLDTFQFYLEKQGWTLVNRSAIKNMTV
                     EQLLKIHC"
BASE COUNT          649 a          639 c          612 g          755 t
ORIGIN      
        1 cggaattgcc gtttccactg catcagtttc tggtactggc gacgagagag tatcagttgc
       61 gctctgattt cttccttcat cttcaaacac gccctttgtc gtcaggtatt cgcagatata
      121 tttgttcagt gctacgggat ctttgtgaat gtcgatcgga cgttcacgga caaggccaaa
      181 aatagtctgg cggtcgtagc gaagggcatc aggctgtttg cgcattgatg ccgagatacg
      241 cttccagtct tcgcggccgt tgtcgataac ttcatttttt gcccagcgat ggatgctgcc
      301 gtcaatgttt ccggcatcca catcaccagg ccagagagcg taggccagtt cgtcatccag
      361 tgttttccat gtctgcttgt attcgcgatg aatggcagca atgaccgggc tgatttttcc
      421 tgttgaattt tcagtgtact gttgattggc tctggcgcgg gcgagatcaa caacagacgt
      481 gtattttccg gtttccttgc gttcaccttc gcgacgtttt ttccagatgc gcatctctgc
      541 ctgaatttcg ggccatttag caccaggaat acatttatgc ttaacccacc caatggcgtg
      601 caacttaagc tccggataca tggcgttaac ttctggcatt ttcatcaacg cttcaacgat
      661 atgtccgtcg aatgttgcca tgtcttcctg caacaattcc tgtgcgctaa taaccatatc
      721 aacggtgatg ttttcacatg tgtcgaactt aaccatgaca gcgttctgta cttcaggggc
      781 cagcttgtca aaagtgacgt tcatcggata ggattcagtc tcaaccggga caaaagaagc
      841 agactcctca tcccagcggt tttcctgcat atattcagca tcccatgaat cgagggcagg
      901 gcggggtatg ccaggtttat cctcgcagac aataaattta taagcgcagt cctgagcagc
      961 cggataatgt tccaggaact gccagtgaaa ttttgctcga gcacggcgtt cgtcgccagc
     1021 ttcaatggct gtggctacag ccacagcgcc ttcttccctt gttgccagtt cgtcaggaat
     1081 agcggcgcaa ataaagactt tactcatttt gttttaacct catgacagat ttaaggatga
     1141 acaaatctct gccattgctg gcatataaga atgaaaccgg atatttatta cggaactgtt
     1201 ttaaagacct gccgggattt cgatattatc ctggtgaata actttatcga ccgggtaaca
     1261 gttaccggga attttctgtt cggttgctgc agtcatacac tcctgcattg tcctgtgaac
     1321 actgactgca atatcaactg gctctccgga aacaagaaaa actgtcagaa caagcgcaaa
     1381 tgctgaattc attgtgcaca tccttttggc atcagacgta aacgagccag cattgaaaca
     1441 atgcatattt tatttaatag ctcccgttct tgttttctct tgttaatggc atcttcagta
     1501 aatacagggt tactgatagt gacaccaatt tcaaaacaac cttcagacgt attaacgttt
     1561 ggtaataacg tttccattat cgcgtcctca acaatgaatt tttagtaatt gctccacagt
     1621 catattttta attgcgctcc ggtttacaag agtccagcct tgtttctcca gataaaactg
     1681 gaaggtatcc agggtgcaga ccattgcgcc gtccggaacg gtttcagtga atttgatatt
     1741 gccgtgttcg tcgagacgga taaccagggt acgtccgtcc ccgtgaatca ttttgtcggg
     1801 agacggggcg ttattctggc gcagttcttt ctccatgcga tcgaactcag cgatgtaggc
     1861 ttctttaaat gcagcggctt ttttgctggt gaagcccatc accaggaaaa cgaagccgtt
     1921 tttggtgatt tggtacattg ggagtttgcg accggttgag tcggtatatt cgctcgacac
     1981 aaaattgtgc tcagtgaatt ttgctgaaca gtccagattg cgaattttat ccaacactcg
     2041 ttcgtggcgt ttaccaaaga actcagcgat cgcaatagac gtagtgacag cgcgtccatt
     2101 ttcgatggtt acgtcagggt gagaaagggt atggatagta gccatgatgg cagcctcgat
     2161 agtcagttta aataactcac caccaaggct ttccacgacc ataagggtgg tgagacgtac
     2221 aggggtggaa ataccggtga ctaccgaaac cggccagcct ttcggctgcc ctgcacgccc
     2281 caccataatc tgaatgtggc tgtgcattac gcataaaaaa accgcatgag cgcggttatg
     2341 cgcagtagtc atcatcgggt ttccacgccc ggcacccgct ttatgaggtg caggtgcact
     2401 ataattccac ccgttctggt tttcaatagc tacattcaac attttctctt acccttcatc
     2461 accgaagtga actttgttga tgcagtgcct ggtgcctcca ggtgacgtta accagttaac
     2521 aattaacgcc ggatacagag aatccaccca taacactgtt tttggtttta actgttccgc
     2581 gtgcgctgag ccgcattcac cgcatcacaa aattcacttt aaaaagggcg gacatcagtt
     2641 catgggcaaa cagat
//
LOCUS       DAZKHC020000254         2653 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZKHC020000254 DAZKHC020000000
VERSION     DAZKHC020000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.255"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            230..325
                     /locus_tag="ACHOSM_RS24720"
                     /old_locus_tag="pgaptmp_004944"
                     /pseudo
     CDS             230..325
                     /locus_tag="ACHOSM_RS24720"
                     /old_locus_tag="pgaptmp_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            551..799
                     /locus_tag="ACHOSM_RS24725"
                     /old_locus_tag="pgaptmp_004945"
     CDS             551..799
                     /locus_tag="ACHOSM_RS24725"
                     /old_locus_tag="pgaptmp_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4561248.1"
                     /translation="MRVEICIAKEKISKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(796..882)
                     /locus_tag="ACHOSM_RS24730"
                     /old_locus_tag="pgaptmp_004946"
                     /pseudo
     CDS             complement(796..882)
                     /locus_tag="ACHOSM_RS24730"
                     /old_locus_tag="pgaptmp_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            967..1041
                     /locus_tag="ACHOSM_RS24735"
                     /old_locus_tag="pgaptmp_004947"
                     /pseudo
     CDS             967..1041
                     /locus_tag="ACHOSM_RS24735"
                     /old_locus_tag="pgaptmp_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            complement(1171..2184)
                     /gene="nleC"
                     /locus_tag="ACHOSM_RS24740"
                     /old_locus_tag="pgaptmp_004948"
     CDS             complement(1171..2184)
                     /gene="nleC"
                     /locus_tag="ACHOSM_RS24740"
                     /old_locus_tag="pgaptmp_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HGU4561249.1"
                     /translation="MYKTILSINMKIPVLQPGFNFFAPAGYSAAVAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVISAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEEVHIGCIKYRNEYELNGESAIKIDDIQSLTCNELYGYDVGQEPILPICEA
                     GENENEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGASDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYADPEREAHLRLRNLNALRQAAMRHEKNERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGVAIDENLQDVNQIQLYHGAPYIFTFGDVD
                     RHN"
     gene            complement(2588..>2653)
                     /locus_tag="ACHOSM_RS24745"
                     /old_locus_tag="pgaptmp_004949"
     CDS             complement(2588..>2653)
                     /locus_tag="ACHOSM_RS24745"
                     /old_locus_tag="pgaptmp_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4561250.1"
                     /translation="VVRPLQILRADGTWENIGGMK"
BASE COUNT          762 a          616 c          514 g          761 t
ORIGIN      
        1 tagattttac aaaactcaat aacatattat atttaaagga aaaatacaga atttacgatg
       61 cttttttata cagatctctc gtgtttttaa ctgattgatt ttgttgaact ctttttttgt
      121 tttgcaggag ttactcctct taccttattg tccactcgat gaccaacgca ttcacaaccc
      181 aaactagaat atcctatcta tgaagcatgt atccgcacgc agattaatta tggtttacat
      241 tgttctacca caatagtcgc atcctaaatg ggtgcggttg aggatcacca tccttgaaga
      301 ggatgcccgc cactttaact gcatgacgca acatttttaa aaatcgacga agatcgtcca
      361 ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac gaacttcgac
      421 gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata aacaatatca
      481 ggaattgaca ctggttatcc atacagataa aaataacacc tgtatataac cacagttaca
      541 ggagggtgtt atgcgtgttg agatctgtat tgctaaagaa aaaatcagca aaatgccaaa
      601 cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac gttatgacga
      661 tgtagaggtg atcgtaaaag ccaccagcaa cgatgggctt tctgttacac gcaccgcaga
      721 taaagattct gcaaaaactt ttgttcagga gacgctgaaa gatacctggg agtctgctga
      781 cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat cattcaatac
      841 tcgcactatc gaaagttcac cagccaaccg cgacacgttc ttacatacga agtgccgcgc
      901 tttccttaat aattttttag cagtactgtg taaatgatga gcgacctaat ccatgcatga
      961 cgcgtagtgc ctattgtgca tctttctgcg tctcttttta ctggacccaa gccaatgatg
     1021 ctggcgatac cattcccgcc aagcccactt acaacagtaa aattcggggc tctgccttta
     1081 catacaatac aatgttgcac gattgcccat catcaactcc gttaatatct atgagtagta
     1141 accctatgta actgtaaaga cgaatcagcg ctaattgtgt ctgtccacat ccccaaatgt
     1201 aaagatataa ggagcaccat gatacagttg gatctgattt acatcctgta aattctcgtc
     1261 gatagccacc cctggaaaat catgttgctg gataaatccg tatcctatgt tagacacagc
     1321 ggaatactct gtgaaatcag gactcgcctc atatcggtca ctgatcgtac ccagtctttc
     1381 gaagaaagcc ctctcattct tttcatgcct catggcagcc tgtcgaaggg cattcaggtt
     1441 acgtagtcta agatgagctt ctcgttctgg atctgcatat cctttgaagt cgggaacact
     1501 ccatcccagc tcttgagcga cacgacgtgc gagaatctcg gtgggtccta gctctatatt
     1561 actatctcca gatggatcgc tggccccagt aacatgatga ataatctcgt gaatcagtcc
     1621 ttcctgccac gatggcatct cataagaatc agtatctggc gcaacactaa aactgacata
     1681 aggctcttcg ttctcgtttt ctcctgcctc gcaaatgggg agaattggtt cttgcccgac
     1741 atcgtatcca tataattcgt tacaggttag tgattgaata tcatcaattt tgatagcaga
     1801 ttctccgtta agctcgtatt cgtttctgta tttaatgcaa ccaatgtgta cctcctcatt
     1861 atgaatgcca tagcttacgg catcacgaaa tgtttgtgat cggctcaacg catcaagtac
     1921 agtgttgcca atcatatcga ccgtatgctg atcgatgagc ctgctacggc tgtcatggac
     1981 ggcgctaatt acactttcgt atacgttgct taaatctgct gcggaaagcg gtattcgctt
     2041 gcctatatcc aaaacgtaat ccgcataggc attttcagca cgattaggag caacggcagc
     2101 agagtatcca gcaggggcaa aaaagttgaa gccaggttgg agaacgggaa ttttcatatt
     2161 gatacttaat atggttttat atatttcagt gccaccaatt cactagctta caaccagaaa
     2221 ctaaaaaaac gtcagggcgt tgataataat gaataccaac tttctgccca tttatagtaa
     2281 tatctttcgt tatcaggttt tgtctggcag cttcacgtaa tatttcaata ctctgcaaag
     2341 caacatttga tgaaatgctg gatgttatca atggcatatg tcatttaccc caacaagact
     2401 aaattttaga aaaaacaact gccgggacaa ctttcgcccc ctgtaacccc aaataagatg
     2461 tcagatttat cggcattaat ttatctccct gttgaattag ctcctctatc atctacttaa
     2521 caaatatccc ccggacattg caacacaaaa accggagccg gactccggtt tttgtgaatc
     2581 cgtcgggtta cttcatcccg ccaatatttt cccacgtccc gtcagcacgc aggatttgca
     2641 gcggtcttac cac
//
LOCUS       DAZKHC020000255         2650 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZKHC020000255 DAZKHC020000000
VERSION     DAZKHC020000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.256"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..156
                     /locus_tag="ACHOSM_RS24750"
                     /old_locus_tag="pgaptmp_004950"
                     /pseudo
     CDS             1..156
                     /locus_tag="ACHOSM_RS24750"
                     /old_locus_tag="pgaptmp_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            233..574
                     /locus_tag="ACHOSM_RS24755"
                     /old_locus_tag="pgaptmp_004951"
                     /pseudo
     CDS             233..574
                     /locus_tag="ACHOSM_RS24755"
                     /old_locus_tag="pgaptmp_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
     gene            complement(695..958)
                     /locus_tag="ACHOSM_RS24760"
                     /old_locus_tag="pgaptmp_004952"
     CDS             complement(695..958)
                     /locus_tag="ACHOSM_RS24760"
                     /old_locus_tag="pgaptmp_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561251.1"
                     /translation="MNEENTALFDRIFTLELQVGFLLPKIIKAMDALGKNNAVSDYLI
                     AEMEHLIKEIPKTDARDDKRFLRAAENALSTVKRSLDAPQDQE"
     gene            1084..1617
                     /locus_tag="ACHOSM_RS24765"
                     /old_locus_tag="pgaptmp_004953"
     CDS             1084..1617
                     /locus_tag="ACHOSM_RS24765"
                     /old_locus_tag="pgaptmp_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4561252.1"
                     /translation="MNAKIRYGLSAAVLALIGAGTSAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKEKCDQVNAIERDKALEWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1773..1955
                     /locus_tag="ACHOSM_RS24770"
                     /old_locus_tag="pgaptmp_004954"
     CDS             1773..1955
                     /locus_tag="ACHOSM_RS24770"
                     /old_locus_tag="pgaptmp_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561253.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            1968..2099
                     /locus_tag="ACHOSM_RS24775"
                     /old_locus_tag="pgaptmp_004955"
     CDS             1968..2099
                     /locus_tag="ACHOSM_RS24775"
                     /old_locus_tag="pgaptmp_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561254.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            2327..2512
                     /locus_tag="ACHOSM_RS24780"
                     /old_locus_tag="pgaptmp_004956"
     CDS             2327..2512
                     /locus_tag="ACHOSM_RS24780"
                     /old_locus_tag="pgaptmp_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4561255.1"
                     /translation="MRELKMKLCVLMLPLVVSACASTPTVQAPCVKPPSPPAWIMQPV
                     PDWQKPLNGIISSSENG"
BASE COUNT          700 a          568 c          736 g          646 t
ORIGIN      
        1 atgtcgatta aacattacga tgttgtcagg gcggcgtcgc cgtcagacct tgcggaaaag
       61 ctgacacaca aactgaaaga gggctggcag ccatacggcg gaccggttgc cattacgccg
      121 tacacactga tgcaggcggt ggctattgaa ggagagccac aggtcggccc ttctaacctg
      181 tatttcaaaa tcagagagga ccgtcgtaag gcggcgcggg gagagtaaag cgatgaagaa
      241 aaaatacgaa ctggttgtta aagggataaa taattacccg gataagatta ctgttactgt
      301 ggcaccggaa attggtgggt atccgtcact gttgttgcca gatgtggcga ttagtcttga
      361 ccgtactgaa ggagccacgc tggagtttta cgaagctgag gcgaaaaagc aggcgaagca
      421 gtttttcatg gatgttgctg ccgggttatg tgaaggggat ggtccgttgc cggaaaagcg
      481 gcccatcatt ttagaggcgc aggacgtgtt gataacctac agaggaaagc taccgggaag
      541 aattactggt tctctgaaga cgccaccgac ggcattgcgg tcagaaaaag atgatattga
      601 atcacgtatt gaacaactgg agtgctatat ctctgaattg aaaaaagcac cccaacaaaa
      661 gatgaggtac ttgcattaga cgaaatgaaa gaaactattc ttgatcttgc ggcgcgtcta
      721 agctgcgctt cacggtcgaa agagcatttt cagcagccct gaggaatctt ttatcatcac
      781 gagcatcggt ctttggtatt tctttaatga gatgttccat ttcggcgatc aggtaatctg
      841 acacagcgtt attcttacca agtgcatcca tggctttgat tatctttggg agaagaaaac
      901 cgacctgtaa ctcaagagtg aaaattctgt caaaaagcgc tgtattttct tcattcatat
      961 ctgttcatta tctttgcgag gcagccatct cacattgttg ggtttacgcc agtgcccacc
     1021 accggcgggc tgaagactta acatatccag ggggccggaa ccgataaatc ctgataaata
     1081 tccatgaacg caaaaatcag atacggcctg tcggctgccg ttctggcgct gattggtgca
     1141 gggacgtctg cgcctgaaat cctcgaccag tttcttgacg aaaaagaagg taaccacacc
     1201 acggcatacc gtgatggtgc ggggatctgg accatatgcc gcggtgccac ccgagtggat
     1261 ggtaagcctg tcgtcccggg tatgaagtta tcgaaggaaa agtgcgacca ggttaacgcc
     1321 attgaacgtg ataaggcgct ggagtgggtg gagcgcaata ttaaagtacc gctgaccgaa
     1381 ccccagaagg cgggtatcgc gtcattttgt ccttataaca ttggccccgg taagtgtttc
     1441 ccgtctacgt tttacagacg gattaatgca ggtgatcgaa aaggtgcctg cgaagcgatt
     1501 cgctggtgga ttaaggacgg tggcagagac tgccgtatcc gctcaaataa ttgctacggt
     1561 caggtatccc ggcgagacca ggaaagtgca ctggcgtgct ggggaattga cagataagca
     1621 gaatattttg ctgaaaaatg acgttggcca acgcgggcgg ataacacgaa atcctgcgaa
     1681 ctggcaaaaa ctaagtgaat aaaagtaaaa ccccgtttgt tggccgcaaa cggggttttg
     1741 tgtttctgac cttgagtaag gcaagggaga acatgggaaa gtataaacga attctgttga
     1801 gattgactat gaaaaacggc cttgaactga aagcgcctgt aactgatgac gtcagcagag
     1861 cgctggcttt tgctattaag tgggtggcgg tcggtattgc tgtgtctccg atgctgtatg
     1921 ggctggcaaa actggtcatt gcgttgaaat cgtgaagagg attaagcatg tcagacaagc
     1981 tcataacgct ggcgaagatc ctctgtgtaa tcgtcggcat ttcattttca ctaatgctgg
     2041 ttgctatttg cttttccact gcctggcgcg tcttaacttt gtcatggctg gtggggtgag
     2101 ggggatatga accgtgttct gtgtgtggtg attattgtcc tgctggtagc ctgtggtgcg
     2161 cttagtctgg ggctgaatca ttaccgcgat aacgccatta cctacaaagc gcaacgcgat
     2221 aaaaaagcca gtgagctgaa gctggcgaac gtgacaatta ctgatatgca ggtacgccag
     2281 cgtgatgtcg ctgcacttga tgccagatac tcgagggaat tagccgatgc gagagctgaa
     2341 aatgaaactc tgcgtgctga tgttgccgct ggtcgtaagc gcctgcgcat caacgccaac
     2401 tgtccaggct ccttgcgtaa agcccccatc acctccggcg tggataatgc aaccggtccc
     2461 cgactggcag aagccgctga acgggattat ttcatcctca gagaacggct gatggcaatg
     2521 cagaagcaac tggaaggagc acaggaatat atccgtaccc agtgtatacc gtgatgtttt
     2581 gttatgaagg tgttactggt aacgttaagg taatttaaca aagagtcagt tccggacttt
     2641 atagtgtgct
//
LOCUS       DAZKHC020000256         2650 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZKHC020000256 DAZKHC020000000
VERSION     DAZKHC020000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.257"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(278..871)
                     /gene="tehB"
                     /locus_tag="ACHOSM_RS24785"
                     /old_locus_tag="pgaptmp_004957"
     CDS             complement(278..871)
                     /gene="tehB"
                     /locus_tag="ACHOSM_RS24785"
                     /old_locus_tag="pgaptmp_004957"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HGU4561256.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKLGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(868..1860)
                     /gene="tehA"
                     /locus_tag="ACHOSM_RS24790"
                     /old_locus_tag="pgaptmp_004958"
     CDS             complement(868..1860)
                     /gene="tehA"
                     /locus_tag="ACHOSM_RS24790"
                     /old_locus_tag="pgaptmp_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HGU4561257.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVMSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            1984..>2650
                     /gene="ydcK"
                     /locus_tag="ACHOSM_RS24795"
                     /old_locus_tag="pgaptmp_004959"
     CDS             1984..>2650
                     /gene="ydcK"
                     /locus_tag="ACHOSM_RS24795"
                     /old_locus_tag="pgaptmp_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HGU4561258.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGVAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEE"
BASE COUNT          748 a          648 c          607 g          647 t
ORIGIN      
        1 tactttttaa gttatagcaa aaagcgccgc ttcgtgttgt ggcaaaaaaa gtgttggtga
       61 aaataaattt ttcagcccct tttcagacga catagtcaat gccgaaaaag gggctaagtc
      121 aatcatcagt agttaaattt cagtaagtta agctgctaaa aaacttcgac tactgtgatt
      181 aatttcacat cccttcgata aaaaatatgc ttaccgaaaa gaagaacagt aaatcatctc
      241 agcgtgggga attttcacgc gccttattct tagcgggtca ttttttacgt gccagcatcg
      301 tggcgaaacg cagtttaata cgattaccgt tggcgtcggt gcggtgcagc tcgccgacgt
      361 cttcattgta tttcaccctc tcccagcctt cgtaatatcg acgtaattct ccctctttga
      421 aggcaaacgg gaagccgacg gtacatggat aatcagcggt atccatcgcc gccacaatca
      481 ggttgtaacc accaagttta gtgcaacgtt gcatattggc aatcagccct gggatggttt
      541 tagcctcaag gaacatcagt accacagtcg aaagaataaa atcgtactgt ctatcaaatg
      601 tgaggttatt cagatcaacg actcgggtgt gtaaattatc cagattttca atggatttaa
      661 tgcgctctac attggcgata ctcatggcat ttttatccca tgcgtcaaca tcataaccat
      721 tggctgccag gtaaagactg ttacgaccat tgccacagcc cagatccagc gttttacccg
      781 gtttaaccac tttcaccgct tccagtactt cagagtgtgt gcgggttaat tcatatttat
      841 cagtaaaata gttttcgtca cgaatgatca ttctttgtcc tctgctttca ttaaaacggc
      901 gcgctcggtt ctgactaaca attttccctg catcagaagc gcaaaagtac ggatgagcag
      961 tattgcaata ataaaattgg taaagataaa cagcggtacc gccagcgtat ggaaaaatcc
     1021 attttcgctg ccactcccca gatgcaaacc ggtggttgcc agtgcagata cgccgaacga
     1081 gaaactccag aatgaagcat taaatggctg ggagagatac catggcatca gacgtagcat
     1141 aaacagcagt tgcagcagtc cataaccaaa aagcattttc gccagcgtat caccctcgcc
     1201 gccgttgacg ctcaaccagg cactacaggc caccagcgca ggagcgagct gaatgccgag
     1261 tgatgtccgc agtgccgtgg gtaattctcc cgaactgcgc agacgctgca agatcacagg
     1321 ttcaaggctt agccatgaga aaacgcctgc gcctaaaaac accagaccgg cgtcggtgta
     1381 gcccaacgca ccacaggcca ttgcgctgat aaagttgttg gcaactgtcg gcagatacag
     1441 tccaggcgtg gtagcttctt cagggtgaga tccgcgccat aatcccgcag tttgccaggc
     1501 ggcataagcc aactgaacca cgacaccaaa actgaacagg cacaccgcca gtgggcgaaa
     1561 ccacggaaca aaaccaatcg ccaccagcat cgtcgttgcc ggaaacaaac tcacaaaact
     1621 gctcatcact ggatggcgca cttctgccag cacgctatgc ggaaagcgta taagtcgggt
     1681 aataaatgcg ctagttaaca atccccagat gatcatcgcc agaatcacca gcccatcccc
     1741 taaccagtgg ctgacctgcc aaacctggct ggcatagcgc caggcaaatc ccatccctat
     1801 cgtccccaac acaataccaa agtagcctgc cggcaaattg agcactttat cgctctgcat
     1861 cttcttactc attttgttta atttataaag tatatttgaa atgcatttta tggaattttc
     1921 tcgagcatag ccagagccgc agaatttgct acggttatag taagtcacct gtgggaaaac
     1981 gctatgcgca aatatcgtct aagtgaagaa cagcgagcct ttagttatca ggaagatggc
     2041 actaaaaaaa atgtgttatt acggcagatt atcgccatta gcgattttaa cgatgtgata
     2101 gcaggaaccg ctggcggctg gatcgatcgc gaaacggtgt tagcacagga aggaaattgc
     2161 tggatttacg accagaatgc tattgcgttc ggcggagcgg tgatttccgg taatacgcgc
     2221 atcaccggca ccagcgtgct gtggggagag gtttacgcaa cggataatgt ctggatcgat
     2281 aatagtgaaa tcagccaggg tgcatatata agtgacagcg tcaccattca tgactcactg
     2341 gtttgcgggc aatgtagaat atttggtcac gccttaatta atcaacattc tatgatcgtc
     2401 gccgcacaag gcttaacgcc cgaccatcaa ctcctgctcc aaatttatga tcgcgcaagg
     2461 gttagcgcct cgcgtattgt ccaccaggca caaatttatg gcgttgccgt cgtccggtat
     2521 gcttttattg aacatcgcgc cgaagttttt gattttgcca gtgttgaagg taatgaagag
     2581 aacaacgtct ggctatgcga ttgcgctaaa gtctacggtc acgcgcaggt gaaagctggc
     2641 atagaagaag
//
LOCUS       DAZKHC020000257         2635 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZKHC020000257 DAZKHC020000000
VERSION     DAZKHC020000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.258"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            233..361
                     /locus_tag="ACHOSM_RS24800"
                     /old_locus_tag="pgaptmp_004960"
                     /pseudo
     CDS             233..361
                     /locus_tag="ACHOSM_RS24800"
                     /old_locus_tag="pgaptmp_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            346..894
                     /locus_tag="ACHOSM_RS24805"
                     /old_locus_tag="pgaptmp_004961"
                     /pseudo
     CDS             346..894
                     /locus_tag="ACHOSM_RS24805"
                     /old_locus_tag="pgaptmp_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            926..2218
                     /gene="sctN"
                     /locus_tag="ACHOSM_RS24810"
                     /old_locus_tag="pgaptmp_004962"
                     /pseudo
     CDS             926..2218
                     /gene="sctN"
                     /locus_tag="ACHOSM_RS24810"
                     /old_locus_tag="pgaptmp_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
     gene            2208..2635
                     /locus_tag="ACHOSM_RS24815"
                     /old_locus_tag="pgaptmp_004963"
                     /pseudo
     CDS             2208..2635
                     /locus_tag="ACHOSM_RS24815"
                     /old_locus_tag="pgaptmp_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
BASE COUNT          854 a          456 c          616 g          709 t
ORIGIN      
        1 tgtagtgaaa atagttacca aacaagtgtt ataagaaata cgaggtgtgt ttaagtaaaa
       61 catatcaaca gttacaaatc tgtatatgga caggatgacg tactagtgta agtggctgat
      121 agcaattcgc tcataacaaa atcattattt tataaatagt aatcatcact ttttaatcat
      181 gtttttatag caagtttatg gagaatgaga atatggaata aggagatgaa acatgattga
      241 agaagggctg ttacttccaa tgaatgtttt gcttcaagga cagaagttaa ccctactgga
      301 ccctgaaatg tggttcttac ggggaaatga aaacaaggat gtaactatat tcttgatcaa
      361 ttagagatga agtatcctga tctattaaaa gagatattaa gatatataac cgtgcagcgc
      421 atttcagagg taattcagcg tctgatccag gagcgaattt cagtgcgcaa tatgcggtta
      481 gttatggagg ccttagcatt atggagccca cgagagaaag atataatcac actcgtcgag
      541 catgtacgag gtgcattagg tcgctacatt tgccataagt tttcatattc tggagaaatt
      601 aaagcaattg tgatttcgcc tgaaatagag gacagaatca gagatggtgt caggcctacc
      661 gctggcggta cttttcttaa tctcgatgca tccgaagctg agatgattct ggataatttt
      721 aaactggcac tttctggaat caatattcct attaaagaca ttattttact gggatctgtg
      781 gatattcgtc gttttatcaa aaaacttatt gaatctagtt atcgagatct cgaagtactt
      841 tcgtacgggg aactaacgga aaatgttccc gttaatattc tgaaaactat ttaggataat
      901 gtcttgcgat atggagcatg ccggtatgaa aaaattaaaa ttattaaata aatacagctg
      961 cttacatagc ataaacggct ctctgattga agcagaactt gacgacgttt cagtgggtga
     1021 agtatgcgaa atttatgcaa gctggcaagc aaatgaacgt atagccaggg ctcaggtcgt
     1081 tggctttcgc aatggaaaga cattattaaa ccttataggc agctcagtgg ggcttacacg
     1141 tactgcagtt ttgaaaccaa cgggagagca gttaaccata cagatcagtg atgcctttct
     1201 tggttcggtt ttgaatgcct ctggtcaaat tatggaaagg tttgtcccgg accctccagg
     1261 cgatagagga aatttacgcc tgattgatga actgccaccg tcgtatcagg aacgtcgagt
     1321 tattaataca ccactagaga cacgcataag agtcattgat ggcgtactga catgtggcat
     1381 tggtcaacgt gttgggattt ttgcatcggc tggctgtggc aaaacggttt taatgcatat
     1441 gctcgtaaac aatactgaag ctgatgtgtt tgttattggc ttgattggcg aacgtggtag
     1501 ggaagttacg gaatgcgcgg aatcgctgaa acaatcagta aatgcggcaa aatgcgtatt
     1561 ggtttatgcc acctctgatt tctcgtcagt agatcggtgt aatgccgccc tgatggcgac
     1621 aaccgtggca gagtatttta gagatcgagg aaagagagtt gtacttttta tcgactcaat
     1681 gactcggtat gcatgagcac tacgtgatat gaaactggca gcgggtgaac cacctgccag
     1741 aggtggttat cctgcatctg tttttgacag tttgcctcgc ttactggaga ggccgggacc
     1801 aacactaaaa ggaagtatca ccgcgtttta taccgtgtta ctggaagggg aagacgaatc
     1861 agaccccctg ggagatgaaa ttcgctcaat cctcgatggc catatctatc tgagtcgcaa
     1921 actcgctggg caaggtcact atccggcaat aaatgttcta aaaagtgtga gtcgagtatt
     1981 tggtcaggtc actgatgaaa aacacagaga taatgctgca cgggtaagga aaatcctcac
     2041 aactttggaa gatctacagg tgtttattga cttaggggaa tatcgcgctg ggcaaaattc
     2101 tgaaaatgat ttcgctatga atgcacggcc taaactcaca aattggttga aacagtcagt
     2161 taatgaaaaa atgccaatga gtgaaactct aaaagagctt gaacgaattg ttaagtaaag
     2221 ttaatcgact tatcagaaga acagcgcaga gcctggctgc atgtgacgca tcattacaaa
     2281 agttaaatgc agaaaaagaa aagttagccg aaaaggaacg cctgtatgat atgcaactta
     2341 aaaacctgaa gtcactgtta gataagaagg agttactcgg cgaagttgta tttcgacagg
     2401 atatatttta ctcgttacgt aaagttgcag tcattcagca gcagatcgct gagataaatc
     2461 ttgaaaagca aaaaatagca gagcggagaa aaatacttaa caaggaaata gttcaacagc
     2521 aagcacaaag aaaacactgg tggctaaaag gagaaaagta cgttcgacta aaaacgcgca
     2581 tcaaaaaaac ttttaaatca gatgcttcat caagacgagc ttgaacagga ggaaa
//
LOCUS       DAZKHC020000258         2626 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZKHC020000258 DAZKHC020000000
VERSION     DAZKHC020000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.259"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..350
                     /locus_tag="ACHOSM_RS24820"
                     /old_locus_tag="pgaptmp_004964"
     CDS             <1..350
                     /locus_tag="ACHOSM_RS24820"
                     /old_locus_tag="pgaptmp_004964"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGU4561259.1"
                     /translation="DDTLSISDALSELNHFINRHADNTKYLKVWGNGATFDNVILRGA
                     YERAGQICPWAYWNDHDVRTIVTLGRSIGFDPKMDMPFDGERHNALADARHQAKYVSA
                     IWQKLIPATSTEL"
     gene            387..668
                     /locus_tag="ACHOSM_RS24825"
                     /old_locus_tag="pgaptmp_004965"
     CDS             387..668
                     /locus_tag="ACHOSM_RS24825"
                     /old_locus_tag="pgaptmp_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HGU4561260.1"
                     /translation="MNTLFLLMAEFNTSNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTGKQKGKEHH"
     gene            complement(716..889)
                     /locus_tag="ACHOSM_RS24830"
                     /old_locus_tag="pgaptmp_004966"
     CDS             complement(716..889)
                     /locus_tag="ACHOSM_RS24830"
                     /old_locus_tag="pgaptmp_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561261.1"
                     /translation="MEDQKATKPQLKFDTMKAFAGMGAAVEVLMKAAPNAFTHATVSG
                     KEQQGKLRRRKAA"
     gene            complement(929..1315)
                     /locus_tag="ACHOSM_RS24835"
                     /old_locus_tag="pgaptmp_004967"
     CDS             complement(929..1315)
                     /locus_tag="ACHOSM_RS24835"
                     /old_locus_tag="pgaptmp_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561262.1"
                     /translation="MLAPTGPCNHLHVICNDPVYYPVNDCYCVLVVNISSIKDGVPHD
                     PSCVLNSGDHRFIKHPSYVVYAEAIIWRVDNMVRKQRSGEISVHDDMPEATFNRILDG
                     FDISDEVTPKNLKFKNKYCVSSIDDE"
     gene            complement(1345..1917)
                     /locus_tag="ACHOSM_RS24840"
                     /old_locus_tag="pgaptmp_004968"
     CDS             complement(1345..1917)
                     /locus_tag="ACHOSM_RS24840"
                     /old_locus_tag="pgaptmp_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HGU4561263.1"
                     /translation="MFCEEKVAQMAAYLLLKRGGRMAYLKLMKLLYLSNRKSILKHGR
                     MIGEDSLYSMKFGPVMSNTLNLIRGKAEGIGDYWYNLIETNGHDVLLRSDPREMDADE
                     VFDELSRADIRILDEIYSLYGHMNRFDLANMTHLESVCPEWHNPGNSRKPIDLKEMLI
                     SEGKSEDEANRIIGKMEESQKLKEFSLQLS"
     gene            complement(2170..2274)
                     /locus_tag="ACHOSM_RS24845"
                     /old_locus_tag="pgaptmp_004969"
                     /pseudo
     CDS             complement(2170..2274)
                     /locus_tag="ACHOSM_RS24845"
                     /old_locus_tag="pgaptmp_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
BASE COUNT          802 a          596 c          506 g          722 t
ORIGIN      
        1 ttgatgatac tttgtcgatc agcgatgctc tctcagaact aaatcatttc attaaccggc
       61 acgcagacaa tacgaaatat ttaaaagtct ggggtaacgg agccaccttc gacaacgtaa
      121 ttttacgtgg agcttatgag cgagcaggac aaatctgccc gtgggcatac tggaatgacc
      181 acgatgtacg cacgatcgtt acgcttgggc gttccatcgg attcgacccc aaaatggaca
      241 tgcctttcga tggcgaacgg cacaacgccc tggctgatgc ccgtcatcag gcaaaatatg
      301 tttccgctat ctggcagaaa ttaattcctg ccaccagcac agaattatga ttttcccggg
      361 tgcagccggt tttgatggag aaaattatga acaccttgtt tttactgatg gctgaattca
      421 atacctcaaa cattgaactc tcagcagtta gccaaaagta ctttggtatg agtccagcca
      481 cagcagaagc aaaagcaaac gcttgtaagt tgcccgttcc aacatatcgc atcggcacat
      541 cacaaaaagc aaaacgttgc atcaatattc aggatcttgc ggaatacata gacaaaagac
      601 gagaagaagg acgtatcgag tgggaacagg tcagaacagg caaacagaag ggcaaagaac
      661 atcactaaag aaaaaacccg cctaaaggcg ggttttcaaa aagcaccagc tatgatcatg
      721 ctgctttgcg acgacgaagc ttaccctgct gctctttacc agagacagta gcgtgagtga
      781 acgcattagg agcagccttc atcagaactt caacagcagc ccccatacct gcgaatgctt
      841 tcattgtgtc gaacttaagc tgtggcttag ttgctttttg atcttccata gaaaactcca
      901 gaagctatac cgaaacaatt cctgttgttt actcatcatc aatagatgat acgcaatatt
      961 tatttttaaa tttaaggttc tttggcgtaa cttcatcaga tatatcaaaa ccgtccagaa
     1021 ttctattgaa tgtagcttct ggcatatcat catgaacaga aatctcaccc gatcgctgct
     1081 ttctaaccat gttatccact cgccaaatta tagcttcagc gtaaacaaca taacttggat
     1141 gcttgataaa gcgatgatca ccggaattca agacgcaaga cggatcgtgg gggaccccat
     1201 ccttgatact agaaatatta acaactaaaa cacaataaca atcgttaacg gggtaataaa
     1261 caggatcatt acaaatcaca tgaagatgat tgcatggccc agttggggca agcacagttc
     1321 ctttcctgta tggctgataa tccgtcatga taattgcaaa gaaaattcct taagtttctg
     1381 agattcttcc attttgccaa ttatgcgatt agcctcatcc tcgcttttac cctcactgat
     1441 cagcatttct ttcaggtcta taggcttacg agaattgcca ggattgtgcc actctggaca
     1501 aacgctttct aaatgcgtca tgtttgcgag atcaaatcgg ttcatatgcc catacagaga
     1561 atagatttca tctaaaatcc ggatatctgc acggctcaac tcatcaaaga cctcgtctgc
     1621 atccatttcc ctcggatctg aacgcaacaa cacatcatgc ccgttcgtct ctatcaagtt
     1681 gtaccagtag tcaccaatgc cttcagcctt accacgaatc aagttcagcg tattcgacat
     1741 gactggacca aatttcatag agtaaaggct atcttcgccg atcatcctgc catgcttcaa
     1801 aatcgacttg cggttagaca gatagagcag cttcatcaat ttcagatatg ccatgcgccc
     1861 acctcgctta agaagtaggt atgcagccat ttgagctact ttttcttcgc aaaacatata
     1921 agaaacctca aagatagtgg actttgtaat tattctcgat acttcatggt ttacttcatc
     1981 accaacatgg cttcttatgg tgtgcccact tgggcctaag caaccagtta ccttgtttat
     2041 caagtttata gctggcttta atcatggtca attgaagtga tgagcaaatg atgctttcta
     2101 agaataacag gttcccaata ggctcccaca aagtgtataa ctagttgttt ttcaaaaacg
     2161 atacatccta tcgagcattg gtgcaacgct aaaccgacca ctccagtgaa cgtcagtttt
     2221 ttcaggcatt gcgctggttt ggttgatttt ttgcatttca gaattaccgt gcattttcaa
     2281 atgtagagat tattttatca atatatcatg gggttatgtt attcagcttc actgttcagg
     2341 aggctcaata gcggggtact ataccataac aacaggaagc gcctgtctca ttgcaaaaga
     2401 aaattgagat cctctcaagg catgaagctc tcacgaagtg atggaaataa tcttattagc
     2461 cgttaacttt gttaaggcca atgataaaca atccaggttc gacgataaat aaaaaatcac
     2521 acattaaact ctggtgatat atctccctgc taatagcatt gatagagaaa aaagaaccca
     2581 ataaatattg ggtcctttta tataatgcct tccatactat cgaaga
//
LOCUS       DAZKHC020000259         2578 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZKHC020000259 DAZKHC020000000
VERSION     DAZKHC020000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.260"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            7..222
                     /locus_tag="ACHOSM_RS24850"
                     /old_locus_tag="pgaptmp_004970"
     CDS             7..222
                     /locus_tag="ACHOSM_RS24850"
                     /old_locus_tag="pgaptmp_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4561264.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            226..474
                     /locus_tag="ACHOSM_RS24855"
                     /old_locus_tag="pgaptmp_004971"
                     /pseudo
     CDS             226..474
                     /locus_tag="ACHOSM_RS24855"
                     /old_locus_tag="pgaptmp_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
     gene            1529..2062
                     /locus_tag="ACHOSM_RS24860"
                     /old_locus_tag="pgaptmp_004972"
     CDS             1529..2062
                     /locus_tag="ACHOSM_RS24860"
                     /old_locus_tag="pgaptmp_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4561265.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKKKCDQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            2361..>2578
                     /locus_tag="ACHOSM_RS24865"
                     /old_locus_tag="pgaptmp_004973"
     CDS             2361..>2578
                     /locus_tag="ACHOSM_RS24865"
                     /old_locus_tag="pgaptmp_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HGU4561266.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     EQANATITDMQVRQRDVAALDAKYSRELA"
BASE COUNT          703 a          539 c          698 g          638 t
ORIGIN      
        1 cggggtatgt accagatgga aaaaatcaca acaggtgtgt catacaccac gtcagcggtg
       61 gggacgggat actggttact gcagctgctg gacaaagtct ctccgtccca gtgggtggcg
      121 ataggtgtgc tggggagtct gctgtttggc ctgctgacgt atcttactaa cctgtatttc
      181 aaaatcagag aggaccgtcg taaggcggcg cggggagagt agacgatgaa taaacaatac
      241 gaactggttg ttaaagggat aaatatttac ccggataaga ttactgttac tgtggcgctg
      301 gaaactggtg ggtatacgtc actgttgttg ccaaatgtgg tgattgatct tgatcgtgtt
      361 gaaggtgccc cgctggagtt ttacgaagct gaggcgaaaa agaaggcgaa gcagtttttc
      421 atggatattg ctgccgggtt atgtgaaggg aacgaaccgt tgccggaaaa gcgcctctgt
      481 tcagaagagc ggtataccat acaaattaac aatgcatata acacgataca gtcagaaaaa
      541 gatgatatcg aatcacgcat tgaaaaactg gagaacagcg tcgttgaact gaataaaaaa
      601 ctgtcggtgc agatcccttc cgaaggtgaa aaaaaacgcc gtgatgagca gttcgcggcg
      661 ttttacgatt attgccggaa agtgatgagc agaaatctcg cagagtgttt ccgtattcaa
      721 aaaaacagag agcgctctgc aaatagtgag attctggata attttttaaa ggggacgcat
      781 ctcgccagtg attgtttcat tgctcattct tttggtggct ttcatgaggc catcaaacat
      841 gatgttgaga gaagtccagg tatcctctgt tccattcccg taagcatcga aatagacaca
      901 aatacgataa ccggtgaact cgttaccgcc ggagtcggta tattccgtca tgaggggaac
      961 gatgccaaag gttcctttac aaaaatcggg ccgttctttc tgaaggtaga gcgatagttt
     1021 gttaagtacc catccgacag ctcgctcatt ttggtttatt ttcgtatcgc gaaacaaaaa
     1081 ttcgaagcgg ccagatgacg catgggtgca accaaatacc ggggcgtatt catcaacctt
     1141 tggtgttcct gaaaacatgc agtccgtgct ctcgaatgca gatgaattgc tgttgatcca
     1201 cttaacaaaa gaaaaaaagg tctgaatcgc ttgtctgtct gaatattcaa caaaatcctc
     1261 aaggtgtgtc tcgatcagtt ccgggtgttt acgaaagtca taatattgac cgggacgata
     1321 tttatgagtg gttgttttat cccatggttg tgctctgcgt ccctgtaaac ggaggtctgc
     1381 cgctggatag ataagcataa gtaatctcct ggtggaatgt aatccacgat catccgggga
     1441 aattaagaac ccgccagtgc ccaccactgg tggggtgaag gcttaacata tccagggatt
     1501 cggaaccgat aaatcctgat aaatatccat gaacaccaaa atcaaatacg gcctgtcggc
     1561 tgccgttctg gcgctgattg ccgcaggggc gcctgcgcct gatattctcg accagtttct
     1621 ggatgaaaaa gaaggtaacc acaccacggc ataccgtgat ggtgcgggta tctggaccat
     1681 ttgtcgtggt gccaccaggg tggatggcaa acctgtcgtc ccgggcatga agttgtcgaa
     1741 gaaaaaatgc gaccaggtta acgccattga gcgtgataag gcgctggcat gggtggagaa
     1801 aaacatcaaa gtgccactga ctgaacccca gaaagcgggt attgcgtcat tctgtcctta
     1861 caacattggc cccggtaagt gtttcccgtc gacgttttat aaacgaatta atgcaggcga
     1921 tcgcagagga gcgtgtgaag cgattcgctg gtggattaag gacggtggca gagactgccg
     1981 tatccgctca aataactgtt atggtcaggt atcccgtcgt gaccaggaga gcgcgctggc
     2041 gtgctgggga atcgacagat aagccgaata ttttgctgaa aaatgacgtt ggccaacgcg
     2101 gacggataac acgaaatcct gcgaactggc aaaacctaag tgaataaaag taaaaacccc
     2161 gtttgttggc agcaagcggg gttttgtgtt tcctgacttc ggaaaagtca aaggagaaag
     2221 tgtgtttgat tttagcaaac tgattcggga gattcgagtg atggctgaaa aattatccac
     2281 ctggaagttc atccttatct ggctggtgtt tgtgattatg gcctccggtt atttcatcgg
     2341 tcagatacgc tggtggtgaa atgaaccgcg ttctgtgtgt ggtcatcatt gccctgctgg
     2401 tggcctgtgg tgcgcttagt ctggggctga atcattaccg cgataacgcc atcacctaca
     2461 aagcgcagcg cgataaaaaa gccagagagc tggagcaggc aaacgcaacc attactgata
     2521 tgcaggtgcg ccagcgcgat gttgctgcgc tcgatgcaaa atactcgagg gaattagc
//
LOCUS       DAZKHC020000260         2558 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZKHC020000260 DAZKHC020000000
VERSION     DAZKHC020000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.261"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..504)
                     /locus_tag="ACHOSM_RS24870"
                     /old_locus_tag="pgaptmp_004974"
     CDS             complement(<1..504)
                     /locus_tag="ACHOSM_RS24870"
                     /old_locus_tag="pgaptmp_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HGU4561267.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKN"
     gene            complement(576..1001)
                     /locus_tag="ACHOSM_RS24875"
                     /old_locus_tag="pgaptmp_004975"
     CDS             complement(576..1001)
                     /locus_tag="ACHOSM_RS24875"
                     /old_locus_tag="pgaptmp_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4561268.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTLPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(985..1266)
                     /locus_tag="ACHOSM_RS24880"
                     /old_locus_tag="pgaptmp_004976"
     CDS             complement(985..1266)
                     /locus_tag="ACHOSM_RS24880"
                     /old_locus_tag="pgaptmp_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HGU4561269.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQMLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQPLTRSLIHENQA"
     gene            1366..1785
                     /locus_tag="ACHOSM_RS24885"
                     /old_locus_tag="pgaptmp_004977"
     CDS             1366..1785
                     /locus_tag="ACHOSM_RS24885"
                     /old_locus_tag="pgaptmp_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561270.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASNYETRVLTPRQRIFLDLLD
                     ELPESEADNLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
     gene            2052..2204
                     /locus_tag="ACHOSM_RS24890"
                     /old_locus_tag="pgaptmp_004978"
     CDS             2052..2204
                     /locus_tag="ACHOSM_RS24890"
                     /old_locus_tag="pgaptmp_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001725971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4561271.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LTRNTD"
     gene            2216..>2558
                     /locus_tag="ACHOSM_RS24895"
                     /old_locus_tag="pgaptmp_004979"
     CDS             2216..>2558
                     /locus_tag="ACHOSM_RS24895"
                     /old_locus_tag="pgaptmp_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561272.1"
                     /translation="MEFKDLPVPFQEMASNVVRSQLATLDLSTVEKETIDTISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKV"
BASE COUNT          705 a          619 c          546 g          688 t
ORIGIN      
        1 gttttttacg ccatggacca tagtgttttg atcttcttga tctgtatctt tatctgtatc
       61 tttatctgtc gtgactcgtc gtgacatgtg cgtgacattt cgtgacgcgc cgtgacaatc
      121 gccattttgt tcccgctttc tttccctctc tcgctgcgcc ctcttgcgct ctgcaggaga
      181 ttttgcggtt tgcgaaatat tgccgttgtc ctctttaagc acctggcgtt tttcccatcc
      241 agtgattaaa tcaccatcaa gtacccgccc ctgcatcgtc tgcaaaattg aatcaattac
      301 ctcttctgtc acgtcgagcg cacttgccaa atcttctgtc gtgacatcaa tgtgacctcg
      361 cgtgacattt cgtgacgcgc tcaccaggag gtggatatac actgccatca ctgttgcaat
      421 tggctgccct gacaccctgg caattgttcg ccacttaggg tcatttggca tgtcatgcca
      481 taatctgagc caggcgttag ccatactcac ctcttctgat accgaatctt tttactcacg
      541 agttgccgga agcgattcga tacggctatt gtcagtcaat gtactgccac agcatttcct
      601 gccgggccac cacggttcat ctgattgaaa ccggcgattg ccactgcgac aaaatcatca
      661 gcgtctctca ccagtcgctc ccgcgtctcc accagctccc gaaaataagc tgaactgtgg
      721 ctgcgcattc tggccaccag caaaggtggc attgcctttt cgatcgctgg taacaacgcc
      781 tgaatttttt caactgcatc aggggtgtct ttctctaccc agcggaaaat tttctgggta
      841 ttgcgagcca gggcttccgg atggctgtcg tcatacagtt caggaagcgt catacccaac
      901 tcaaaataag cctgggttat tccagctgct ggaacttttt cgccatcagg acgcgcccag
      961 gcattcatcg ccatgcggat gtgttcatgc ttgattttca tgaatcaagc tcctagtaag
     1021 tggttgtgtt aacgttttgg tatcttccag ctcgggccaa atattcatcc aatcaaaagg
     1081 ccttagttgc tgacgtgtaa cttcaccatt actggctcgc tcaataagga cacataacga
     1141 tgcccctaac atttgacctt tactcaatgc ctttcttaga taaccgatgc tagtaccaca
     1201 ctcacatgca aacatacgct gttcatctga cgaaagagaa ttgagaaata ttcttaattc
     1261 ttccatagct actccttagt aaacacagca aggataccta caggtaaaca aaagtcaata
     1321 cccttgggtt gtttaccttg cggtaatcgc atctattatt tacctatgga caaatatgaa
     1381 tttagacgac aacaactcat caaaattcgt gatgagaaat gcgatggtaa agcggttaac
     1441 gtggccagaa agatcggtcg cgagccttct tatgtatcaa gaatgttgta cccagagggg
     1501 aaaaaaggaa aaaaacggat cgctgatgat atggtggaga ttatagaaga gtcctttggg
     1561 ttaccccggg gatggatgga tggtatcgtt tcatcatcaa cgaacacagc ctccaattat
     1621 gaaacaaggg ttctaacgcc acgacaacgt atttttttag atctcttaga cgaactgcca
     1681 gaaagtgaag cggataactt attaaaaact cttgaagaga aaaaacagta ttacaatatg
     1741 atctacgaag aaatccgtaa aaagaaagca caaaacgcat cataactcac caaacaacca
     1801 gtcaccagtt aagacacatc aaaaaaatta cccatgggta tttacttttt aaatacccat
     1861 gggtatcctt cctttcatac caacccaccc cgtcccacag aacgcagggc aatacttcga
     1921 gttaccaggc agtggtcagg gggtaagtag ccagcccgag gcgtatgaac atgacggcgg
     1981 gaacactttg tataacagcg cagcaggttt ttagttccgc taccccagcg ttaaggggaa
     2041 atgaggtcag catggatact atcgatcttg gcaacaacga atctctggtg tacggcgtgt
     2101 ttcccaacca ggacggcacg tttaccgcga tgacgtatac cagaagcaaa acgtttaaaa
     2161 ctgaagctgg cgcgcgtcgc tggttaacca gaaacactga ctgatgaggt tgacgatgga
     2221 atttaaagat ttaccagtac cattccagga aatggcatcg aatgtggttc gctctcaact
     2281 ggcgactctt gacctgagta ccgtagaaaa agaaaccatc gatactatat ccggtaacgt
     2341 gcgtcgtgcc tttatcggtc tgtacgaaga gaagcagctc tctgataacc aggatttaca
     2401 tgaaaaatac ttcctggaat taatggacat cattaataaa ggatttggct tgttaatgaa
     2461 aaagaaaggg attcgaatag ctccccttga aaatcatttt acagcgagca gtattaattc
     2521 ctgtgattta aagcatcaca catccgatgg gaaagttg
//
LOCUS       DAZKHC020000261         2538 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZKHC020000261 DAZKHC020000000
VERSION     DAZKHC020000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.262"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(204..893)
                     /locus_tag="ACHOSM_RS24900"
                     /old_locus_tag="pgaptmp_004980"
     CDS             complement(204..893)
                     /locus_tag="ACHOSM_RS24900"
                     /old_locus_tag="pgaptmp_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGU4561273.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(908..1030)
                     /locus_tag="ACHOSM_RS24905"
                     /old_locus_tag="pgaptmp_004981"
     CDS             complement(908..1030)
                     /locus_tag="ACHOSM_RS24905"
                     /old_locus_tag="pgaptmp_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HGU4561274.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
     gene            1367..2326
                     /locus_tag="ACHOSM_RS24910"
                     /old_locus_tag="pgaptmp_004982"
     CDS             1367..2326
                     /locus_tag="ACHOSM_RS24910"
                     /old_locus_tag="pgaptmp_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HGU4561275.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
     gene            complement(2470..2538)
                     /locus_tag="ACHOSM_RS24915"
                     /old_locus_tag="pgaptmp_004983"
                     /pseudo
     CDS             complement(2470..2538)
                     /locus_tag="ACHOSM_RS24915"
                     /old_locus_tag="pgaptmp_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
BASE COUNT          832 a          543 c          455 g          708 t
ORIGIN      
        1 caatacaaga tgataacatt ggcttacaac tcataacaaa agcaattcaa tgccgtcaag
       61 agattacagg ctaaaaaaac tctattacat agcagccagc atgtttaccg tacaagtaca
      121 actcagggca taaaaaaaac ccactcggca gcgggtttat acatttttta caacatacca
      181 aatttgcatg aagtatatgg cttttaatcc agttttgcaa tattttgctg taaaaatgct
      241 gccttttgtt ttgaacgtgt tctcgtcaca agcaataaag catcactatc aagctgtaga
      301 aaaatgtgct tcattgcaac ccagcgttca gtaaatgtct cggaccagtt ttttgttgtc
      361 actcccacca atgatgccag tgtctggtat tcataggcct cacgccctgc aagttcgctc
      421 ttcacatcct gtgcagccag ccagattaac gtcttcaggc gatccagtgt cttacctgca
      481 atttttctgt tacttaacaa atctttaaac tcgctccatg cccattgcgt tatggtgacc
      541 tgatgccccc atcgaacgct ttcgctgtaa caccaaagca accatgcttt ctgatgttca
      601 tcgagagaca aaaccgcgcg gcgccatgaa gaggttgaga attcaaccgg gctgaccaaa
      661 gcaatggatg aaccttttgc gtacgactgc ttaccggaag tcggcgtatt atccagcgta
      721 atcatcttgc cagttaccac atccagaatg cgcggcttct ttcgtttgta tgtaccagta
      781 tcaaattgtg catgctcctg ccaggcttcg agctggcctt tcgttgctcc gttcaagtca
      841 gcagtagctg ccataagttg ctcacgaaca tactgtaaat attgggtatt catgcagtaa
      901 atcctttcta tattttggca taattcttca acattcggta atcgttcaaa accgaatcgg
      961 ggaaacgaca taagcacagg agcccccagc gacagcgaag gagttctgat atataagact
     1021 cagacatcat tcattccccg gttctccaaa atctgtttca ctcatcatcc ataactacct
     1081 gtaattgccc cccctttttt tgtaacagtt cttatattgc tatatagaat agccattact
     1141 aatgcattta aatttaataa aataaaaatt ataaaaacat aaaacaccac gcaaacacac
     1201 ttaataaaac accgttacat taaaagataa taaaaaccac aataaaaaac gaataaatca
     1261 attgtctcac gcaattataa aacatcatat tgattacgca ccttgtatta caaactcatg
     1321 tatgtaaaat acgcgcacca ttcaaaaaaa aggaagacaa taacatatga aaaaaagtgt
     1381 catcgctggc gtctttattg ctctgtcatt taccacgtgt tcagctatcg cgaacagcct
     1441 tgcattatca ttagcaaatg atgatgcagg gaagtttcaa ccaatactta atgatattta
     1501 tggcaataaa catgaaaaca gagatgatta ctcacaaggc ttatttctgg gatatagcca
     1561 cgatatctca gactcgagcc aattatctct ccatattgcg caagatattt actctccatc
     1621 aggcagtaat aaaagacaca acacagctgt aactggagac agagctttta gtgcatacac
     1681 tcacactggt attgaatgga actcccttgc gaatgactgg attcgctatc gattaggtac
     1741 tgacataggt gttgttggcc ccgacgcagg cggtcagaaa gtacaaaata aagctcatga
     1801 gattattggg gcagaaaaat atcatgcatg ggatgatcaa atagagaatc gctacggtta
     1861 tactgtaaaa gggatgctat ccatgacacc aagtatggat attttaggtg ctaatgttgg
     1921 attataccct gaagtttctg ctgttactgg aaacttattt caatatgtag catatggcgc
     1981 aaccattgcc attggtaatg ataaaacctt caattcggat aatggctttg gtctgctggc
     2041 tccccgtggt ttaatgcata tgtccgatac aagcggattc aaatacaaga tttttgcagg
     2101 tatggaaaga cgagatgtca atcgcaacta tactctcgaa ggaaaaacaa tacagacgaa
     2161 acaaacaaca gtatcgctaa acaaaactgt tgatgaatat caagttggcg caacaattgg
     2221 gtatgcacct gtagccttca cactagcatt taataaagta acatcagaat tcaagacagg
     2281 ggatgactat tcatttataa atggagcaat caccttcttt ttttaactga attgaattca
     2341 atcaaaataa cataagtcca acaaaaacat aaagtgcgaa atgaatgcta gctccattta
     2401 tttcgcacta taaaagatta aaagttgcaa taaaataata aaatgactca gttacgaaaa
     2461 ccaataaact gtggccagta gtgagtcgct catcatcggg ctttttggcg aatgaaattt
     2521 agctacgctt tcgagtct
//
LOCUS       DAZKHC020000262         2443 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZKHC020000262 DAZKHC020000000
VERSION     DAZKHC020000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.263"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(89..1501)
                     /gene="pykF"
                     /locus_tag="ACHOSM_RS24920"
                     /old_locus_tag="pgaptmp_004984"
     CDS             complement(89..1501)
                     /gene="pykF"
                     /locus_tag="ACHOSM_RS24920"
                     /old_locus_tag="pgaptmp_004984"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HGU4561276.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1509..1703)
                     /locus_tag="ACHOSM_RS24925"
                     /old_locus_tag="pgaptmp_004985"
     CDS             complement(1509..1703)
                     /locus_tag="ACHOSM_RS24925"
                     /old_locus_tag="pgaptmp_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561277.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            2058..2267
                     /gene="fumD"
                     /locus_tag="ACHOSM_RS24930"
                     /old_locus_tag="pgaptmp_004986"
     CDS             2058..2267
                     /gene="fumD"
                     /locus_tag="ACHOSM_RS24930"
                     /old_locus_tag="pgaptmp_004986"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HGU4561278.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          648 a          522 c          584 g          689 t
ORIGIN      
        1 tgatttttta ttgcttctgg ttatcgatta aataaaaaaa gcgcccatca gggcgcttcg
       61 atatacaaat taattcacaa aagcaatatt acaggacgtg aacagatgcg gtgttagtag
      121 tgccgctcgg taccagtgca ccagaaacca taactacaac gtcacctttg tgtgccagac
      181 cgctctgcag agccagttct ttacccagac ggtagaaatc atcagtagaa gtgatctctt
      241 taaccagctg cggcacaacg cctttgctca gtaccaactg atgagccgtt ttttcgttgg
      301 tggtcagtgc caggatggtg gcatccggga agtatttacg tactgcgcga gcagatttac
      361 cgccctgagt agcaaccacg atcagcggag catccagttt ttcagcagtt tcaacggcac
      421 cacggcatac cgcttcggta atgcgcagtt tacggttgtc attgttgaac tcgagacggc
      481 tgttcatcac gcggtcggta cgttcgcaga tggtcgccat gatagaaacc gcttccagcg
      541 ggtatttacc ttttgcggat tcaccagaca gcatcactgc atcagtaccg tcgaggatgg
      601 cgtttgcaac gtcaccggct tctgcgcgag tcgggcgtgg gtttttgatc atggaatcca
      661 gcatctgggt cgcagtgata acgactttac gtgcacggat acatttttcg atcatcatct
      721 tctgggcgaa gataacttct tctaccggga tttctacacc caggtcgcca cgcgcaacca
      781 tgatgccgtc agaggcttcg aggatttcgt cgaagttgtt gaggccttcc tggttttcga
      841 ttttggagat gatgtggatg ttttcgccgc cgtgcgcttt caggtgctca cggatttcga
      901 taacgtcaga acgcttacga ataaaggaag cagcaacaaa gtctacgcct tgttcgcaac
      961 caaagatcag gtcctgtttg tctttttcag ccagtgctgg cagagcaatg gaaacgccag
     1021 gcaggttcac acctttgttt tcgcccaggt caccgttgtt cagcacttta cagataactt
     1081 tgttaccttc aatggcggta acttccatac cgatcagacc atcgtcaacc agtacggtgt
     1141 tgccaactga caggtcagta gtgaaacctt catacgttac cgcaaccatt tcgctgttgc
     1201 cgataacaga tttatcagtg gtgaaagtaa aggtctgacc agctttcaga gaaacgtcgt
     1261 taccgccttc cagtttcatg gtgcggattt ccggaccttt ggtatcaagc aggatagcgg
     1321 cggttttacc agttttgctc atcacgttgc gcagattctg aatgcgctga ccgtgttctg
     1381 catagtcacc atgagagaag ttcagacgca taacgttcat gccagcgtcc agcattttag
     1441 ctaacatctc ttcagattcg gttttcggtc cgatggtgca aacaattttg gtctttttca
     1501 tgacagtctt agtctttaag ttgagaagga tgggagaaac ttgctttctg ggcgcactgc
     1561 cggaaagcga atccggtatt acgaaagtgg tggtgcctcg ctctaaggat aggtgacatc
     1621 gaaaaagcgt gcagaggaat gtgtgcttgt gtctcagccc aacgtgagtg cctgtttttg
     1681 agtcgttgag ttctacagtc caaagtgctg aaaccattca agagtcaatt ggcgcgcatt
     1741 atacgctgaa attaatccaa aaggaaaaca aaaacagcgt ttcaaaaaat atacaacttg
     1801 ttagacaata aattgttatc aaagatttaa cgttccaggg aaaaggttac gcaaaaactt
     1861 cgctgtttgc gcaacgtctg gtgccaaagt tgtcagtgtg aggataagtg aaagttcatc
     1921 cggtgtataa atatatcatc gctcaattgc tatagttaaa ataatttatc gcgttatttg
     1981 tctgattttg tttgattttt gtttaattgg ttagattgat atatgctgat agttggcatg
     2041 ataaataggt ataaagaatg ggaaatcgga caaaagagga tgaactgtac cgagaaatgt
     2101 gcagagttgt cggtaaagtt gtgctggaaa tgcgcgatct ggggcaggaa ccaaagcata
     2161 ttgttatcgc aggcgtatta cgtacagcat tagccaataa acgcattcag cgcagtgagc
     2221 tggaaaagca ggcgatggaa accgtcatta acgcactggt aaaatgacca ttcaggatga
     2281 ggcggtaagc ttcatcctga tattcttgct tcagttgcgg tccagttaat cattctaaat
     2341 aacaactgct gaaattatac gaattgcggt atgtatttta atttataacg ataatattat
     2401 ttttattttc tgcaatgaac gtcacaaata attgtcgctt aag
//
LOCUS       DAZKHC020000263         2404 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZKHC020000263 DAZKHC020000000
VERSION     DAZKHC020000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.264"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1016
                     /locus_tag="ACHOSM_RS24935"
                     /old_locus_tag="pgaptmp_004987"
                     /pseudo
     CDS             1..1016
                     /locus_tag="ACHOSM_RS24935"
                     /old_locus_tag="pgaptmp_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            943..1425
                     /locus_tag="ACHOSM_RS24940"
                     /old_locus_tag="pgaptmp_004988"
     CDS             943..1425
                     /locus_tag="ACHOSM_RS24940"
                     /old_locus_tag="pgaptmp_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGU4561279.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAVIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            1469..2383
                     /locus_tag="ACHOSM_RS24945"
                     /old_locus_tag="pgaptmp_004989"
     CDS             1469..2383
                     /locus_tag="ACHOSM_RS24945"
                     /old_locus_tag="pgaptmp_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561280.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDDLRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
BASE COUNT          729 a          514 c          560 g          601 t
ORIGIN      
        1 cgataagctc cagcaggtgg aactgaagcg cgttccgcgc gtggagaatc tgctctattt
       61 tatgcagaac acgcgtaaac ggcttgaacc ccagctgaag agtaacactg agaacgccgc
      121 acagaccacg gtcagaacgc tgatttatgc cccggaaaca caggcatctt ccacatcaga
      181 agcggtagtg cctcccctgc ccggcctgtc tgatatgaaa gtggaagtgc gcagtctgac
      241 agagaatccc ccacaggcca gtgtgataaa gcaaggcagt acggtaagag ggtttatcgc
      301 agggatcgcc tgttcagtgg ctgtcgcctc agcattgtgg tggtggcaag tctatccggt
      361 gcagcagcaa ctgttacagg ttaacgacac cgctcagggc gcagcaacgg tgtggatggc
      421 ctcacctgaa ctcgaaaact atgagcgcag gctgcaacaa cttcttgata cctccccggt
      481 acagccgctg gaaaccggga tgcagatgat gcgtgttgcc gacagtcgct ggccggaaag
      541 cctgcaacag caacaggcct cgacacaatg gaatgaggca ctcaaaaccc gcgcacagag
      601 tagcccgcag ttgcgtggct ggttgcagac ccgccaggac ttacatgctt ttgcagacct
      661 cgtgatgcag cgcgagaaag agggactaac cctttcctat atcaaaaatg tcatctggca
      721 ggcggagcgg ggactggggc aggaaacccc cgttgagtct ctgttgacgc agtaccagga
      781 tgcccgtgcg cagaagcaga atacagatgc gctggaaaaa caaattaatg agcgactcga
      841 aggcgtgtta agccgctggc tgctgctgaa gaataacgtc atgccagagg cggcaaccgg
      901 taccacggcc gaaaaataat tatttaatta aaggagatta tcatggcaaa tataagttat
      961 ttatctttga gtggagagac acaagggctg atttcagcag gttgctcaac gcttgattct
     1021 gtcggaaaca aagcgcagcc agaacataaa gatcagatca tggtttatgc gttaatgcat
     1081 tccatctcaa gaagtcaaaa tgtcaatcat catgaattaa ttatcaccaa accggttgat
     1141 aaatcctcac cattgctggc aaaagcactt agtgacaatg aaaaaatggc aatatgtgaa
     1201 tttatactct accgaaccag taaagcggtt atttaccagc cttattataa aataaatctg
     1261 tcaaaagccc gaatatcaag tattgatttt gtcacaccgc atgctgtcct ggaaaaagaa
     1321 ctggaaccac aggagagaat tgcctttatt tatgaagata tttcctggga gcatacgtta
     1381 gccggtacga atgcaatgag taaatggcag gatcgtgtac agtaacttac tgtaataaca
     1441 taataaatta ctattgcgga gagataaaat ggctggttta aggaataaca acaacacaca
     1501 gaatgcacag tgggctgatt atgtgggtga cattcttcgg ggggctcagc cgataaacca
     1561 gctagtacct cagcaccctt atcttaacga cgtcccactc attgatgatt taaggcatca
     1621 gaacactcat catgttattc tcttgacact tgatgttgca aaaaaaatac tcagcccaat
     1681 aacatcattt gattacattc attttatcac tacacatcct tcaggtataa aagatacact
     1741 cgcctggtta gttaacgcga gtaaattaat gactgagttt gatgacaatg ggaaaattat
     1801 atttaacctt aatgccttaa agtatacaaa agcaagttac tttgaaatac ttggagagaa
     1861 atatataaaa atcacaacat ccagcccctg gcttttagaa aagctcggta aatatatttt
     1921 ctcttcacga gcgcctcaag ttctggaact cgctattggc tggcgaggag ctttatctga
     1981 atcaataaaa ggagttaagt tctgtatctg gttttcagta gcctggagaa ccatagaatt
     2041 tattatgagt tcagagcgag atctggttaa ttttcttggc gatttttcaa tggatgtggc
     2101 caaagcagtt atagccggtg gtgttgcaac cgctattgga agcctggctt cttttgcctg
     2161 tgttagcttt ggctttccag taattcttgt cggaggagca attttactga cagggatagt
     2221 gtgtacagtt gttttaaatg aaatcgatgc tcaatgccat ttatcagaaa aattaaaata
     2281 tgcaattaga gatggactaa aacggcaaca ggaacttgat aaatggaaaa gggaaaacat
     2341 gactccattt atgtatgttc ttaacactcc acccgtgata tgaggattaa ccatgcgttt
     2401 acat
//
LOCUS       DAZKHC020000264         2342 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZKHC020000264 DAZKHC020000000
VERSION     DAZKHC020000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.265"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..587
                     /locus_tag="ACHOSM_RS24950"
                     /old_locus_tag="pgaptmp_004990"
     CDS             <1..587
                     /locus_tag="ACHOSM_RS24950"
                     /old_locus_tag="pgaptmp_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030598 - rRNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome-inactivating family protein"
                     /protein_id="HGU4561281.1"
                     /translation="TTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSSYLALMEFSG
                     NTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDVDLTLNWGRI
                     SNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNEESQPECQIT
                     GDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            599..868
                     /locus_tag="ACHOSM_RS24955"
                     /old_locus_tag="pgaptmp_004991"
     CDS             599..868
                     /locus_tag="ACHOSM_RS24955"
                     /old_locus_tag="pgaptmp_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU4561282.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            1354..>2342
                     /locus_tag="ACHOSM_RS24960"
                     /old_locus_tag="pgaptmp_004992"
     CDS             1354..>2342
                     /locus_tag="ACHOSM_RS24960"
                     /old_locus_tag="pgaptmp_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4561283.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMT"
BASE COUNT          608 a          518 c          645 g          571 t
ORIGIN      
        1 tgacaacggt ttccatgaca acggacagca gttataccac tctgcaacgt gtcgcagcgc
       61 tggaacgttc cggaatgcaa atcagtcgtc actcactggt ttcatcatat ctggcgttaa
      121 tggagttcag tggtaataca atgaccagag atgcatccag agcagttctg cgttttgtca
      181 ctgtcacagc agaagcctta cgcttcaggc agatacagag agaatttcgt caggcactgt
      241 ctgaaactgc tcctgtgtat acgatgacgc cgggagacgt ggacctcact ctgaactggg
      301 ggcgaatcag caatgtgctt ccggagtatc ggggagagga tggtgtcaga gtggggagaa
      361 tatcctttaa taatatatcg gcgatactgg gcactgtggc cgttatactg aattgtcatc
      421 atcagggggc gcgttctgtt cgcgccgtga atgaagagag tcaaccagaa tgtcagataa
      481 ctggcgacag gcccgttata aaaataaaca atacattatg ggaaagtaat acagctgcag
      541 cgtttctgaa cagaaagtca cagtttttat atacaacggg taaataaagg agttaagcat
      601 gaagaagatg tttatggcgg ttttatttgc attagcttct gttaatgcaa tggcggcgga
      661 ttgtgctaaa ggtaaaattg agttttccaa gtataatgag gatgacacat ttacagtgaa
      721 ggttgacggg aaagaatact ggaccagtcg ctggaatctg caaccgttac tgcaaagtgc
      781 tcagttgaca ggaatgactg tcacaatcaa atccagtacc tgtgaatcag gctccggatt
      841 tgctgaagtg cagtttaata atgactgagg cataacctga ttcgtggtat gtgggtaaca
      901 agtgtaatct gtgtcacaat tcagtcagtt gacagttgcc tgtcagactg agcatttgtt
      961 aaaaaaattt cgcatggtga atccccctgt gtggaggggc gactggtgaa aaatccttgc
     1021 ttgtgattca ttatcgacac gggttcggtg gtaccaggcc gaactcaccg ggaggcaccc
     1081 ggcaccatgc agtatacaga gattaggcat ataccaaggc ctctcatagc aggggccttt
     1141 ttacatgtaa aaaagcccga gtgggttcgg gcaattgcat gagatactcg ttttaataat
     1201 cgaaagcatt ttaaccagga ttcataaggc tgcgcaactg cgcggccttt ttcgtatttc
     1261 gggctgtagt cttccttctg ccattgtcct gtaacttccg gacttcagcc cgctcctcat
     1321 ctgactcaca cattatcccg accgggagga ttcatggcat ttaaacacta tgacgtggtc
     1381 agggcggcgt cgccgtcaga ccttgcggaa cgaataactc aaaaactgaa ggaagggtgg
     1441 cagccttatg gtagtgcgct gatttcgaca gctggttatg gtgcggagtt catccagcca
     1501 gttgtgagtg aggggagcat ctcatcacca gaggagccag gcaaccgtcc gacgacctca
     1561 gcgccttctg ttgcgccaga atattactat gtgatcgcgc ttgctggtca gtccaatggt
     1621 atgtcatacg gtgagggact gccattaccg gatacattcg acagtcctga tccacgaatt
     1681 aaacagttag cgcgtcgcag tacggtgaca ccgggaggtg ccgcctgtaa gtataacgac
     1741 atcattccgg cggaccattg tctgcatgat gtgcaggaca tgagccgcct taaccatccg
     1801 aaagcggacc tgtcaaaggg gcagtacgga accgtggggc aagggctgca tatcgccaaa
     1861 aaattgctgc cgtttatacc ggcgaatgcg ggcattctgc tggttccgtg ctgtcgtggt
     1921 ggttcagcgt tcaccaccgg agccgatggc acatacagtg acgcgagtgg tgcctcggag
     1981 aattcaaccc gctggggtgt ggacaagccg ctgtataagg accttatcgg tcgaacaaaa
     2041 gcagcactga agaagaatcc gaaaaatgtg ctgtttgccg tggtgtggat gcagggggaa
     2101 tttgattttg gcggtacgcc ggtaaatcac gcagcacagt ttggtgcgct ggttgataaa
     2161 ttccgtgcag acctggcgga tatggcaggt cagtgcgtcg gtggctctgc tggcggtgtt
     2221 ccctggatat gcggggacac gacgtatttc tggaagcaga agaacgaatc cacgtaccag
     2281 acggtgtacg gcagctacaa aaacaaaacg gaaaagaata tccatttcgt accgttcatg
     2341 ac
//
LOCUS       DAZKHC020000265         2325 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZKHC020000265 DAZKHC020000000
VERSION     DAZKHC020000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.266"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            213..797
                     /locus_tag="ACHOSM_RS24965"
                     /old_locus_tag="pgaptmp_004993"
     CDS             213..797
                     /locus_tag="ACHOSM_RS24965"
                     /old_locus_tag="pgaptmp_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU4561284.1"
                     /translation="MDKRAKNQIVDSDIARLLLKLRKSRNLTVTELAQRSGVSQAMIS
                     KVERGTSSPSATILSRLANALNITLSKLFAELEMQQNSLVLLADQQQHWIDEETGITR
                     WSLSPAGACPELIKVEIPPMGQLTIPASANEHLSGQTLWMLSGSLDFRVNHQTHHLQA
                     GDCLALTFASEYQMRNPDSGQSCSYIVAFSQKNP"
     gene            complement(822..1259)
                     /locus_tag="ACHOSM_RS24970"
                     /old_locus_tag="pgaptmp_004994"
     CDS             complement(822..1259)
                     /locus_tag="ACHOSM_RS24970"
                     /old_locus_tag="pgaptmp_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU4561285.1"
                     /translation="MLKIRMSRPSEAPEIIQIWKNSVDATHDFLTAHDRQEIEKEVVS
                     FFSETPVLVATNQEDQPLGFMFLHEGHLEAFFVDASARGLGVGKHLISHALALHPDLS
                     VDVNEQNHQAVGFYQHMGFKLSGRSERDNQGRPYPLLHLYKAM"
     gene            1724..1891
                     /locus_tag="ACHOSM_RS24975"
                     /old_locus_tag="pgaptmp_004995"
                     /pseudo
     CDS             1724..1891
                     /locus_tag="ACHOSM_RS24975"
                     /old_locus_tag="pgaptmp_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
     gene            complement(1994..2317)
                     /gene="iraM"
                     /locus_tag="ACHOSM_RS24980"
                     /old_locus_tag="pgaptmp_004996"
     CDS             complement(1994..2317)
                     /gene="iraM"
                     /locus_tag="ACHOSM_RS24980"
                     /old_locus_tag="pgaptmp_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU4561286.1"
                     /translation="MKWIVIDTIIQPSCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNIAPFNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGIK"
BASE COUNT          714 a          431 c          472 g          708 t
ORIGIN      
        1 cgatgattaa cctctatgta gctaggtttt tgtaaagaaa ctgtgcgtca ctgtatccag
       61 gactgttatg tgccaaataa ggaagttgaa gttaccagct cttactctaa agctataacg
      121 tatagaaaaa atgcatttga gacgtgtaaa atcatttatt taatcgcaat ttatgtaatg
      181 gcataatcag gtcggtgaaa gaggggagaa gaatggataa aagagctaaa aatcagatag
      241 tcgatagcga tattgcacgt ttgttactca agctcagaaa gtctcgaaac ttgactgtta
      301 cagagcttgc ccaacgatct ggtgtcagtc aggcgatgat cagcaaagtt gaacgaggca
      361 cttcaagccc cagtgcaaca atacttagtc ggttagctaa tgcactgaat attacgctat
      421 caaagctttt tgcagagctt gagatgcaac agaattcact tgtacttttg gctgaccagc
      481 aacaacattg gattgatgaa gaaacaggca ttacccgctg gtctttgtcc ccagcgggtg
      541 catgtccgga actaataaaa gttgaaatcc cccctatggg gcaattgact atccctgcct
      601 ctgcaaatga gcatctttct ggacaaactc tatggatgtt aagcggcagc cttgattttc
      661 gagtcaatca tcagactcat catttacaag ccggtgattg tcttgctctt acctttgcca
      721 gcgagtacca aatgagaaac ccagatagcg gccagagttg ttcttacatt gtcgctttca
      781 gccaaaaaaa tccatgatgt aattctccta aaattatcat gtcacattgc cttataaaga
      841 tgtaatagag ggtagggtct gccttgattg tcccgctcgg aacgtcctga aagtttaaag
      901 cccatatgtt gataaaagcc tacagcttga tgattttgtt catttacatc aacgcttaga
      961 tcaggatgta gagccagcgc atgtgagatc agatgtttcc cgacgccaag tccgcgagct
     1021 gacgcatcaa caaaaaaagc ctctaaatgt ccctcatgca aaaacatgaa tcctagtggc
     1081 tgatcttcct ggtttgttgc taccagcaca ggggtttctg aaaaaaagct gacaacttct
     1141 ttttcaatct cctgccggtc atgggctgta agaaaatcgt gtgtggcatc aacggaattt
     1201 ttccaaattt ggataatttc tggtgcttca gatggtcgcg acattctgat ttttaacatg
     1261 ttatctccct taaagtggtc gttgcttgtg tcttgagata caaaacgagt catcaaattt
     1321 actatagtga aattaaattc acaatagtga tttggttcct gtgccttaac attacccatg
     1381 aggagtacaa ttaaacgggt taatcagtat ctccatttga aagaaaaaag acttctgtac
     1441 tgtttgatat caccgctatt ttcttatttg ctgtgcaggt cgtagtggga caaaactgag
     1501 acacataagg tctagcaatg gcttgcaagg cctcacatgt attgagatgt taataaaatg
     1561 tagacttgta attttgatat aaatggtaga gaaaatcttt ccccaaaata aaaacgaacg
     1621 tcaatgaaat caaacggttg aataaagttg attttggcta ataaaaagac aataaaatga
     1681 tttttatcat taaatatcaa tgagttgaac tcattgtttt tatatactgt tacgccatat
     1741 gggctggact gaagccgcag acctgattgt taaaggtatg gaaggcgcaa tcaatgcgaa
     1801 gaccgtaacc tatgacttcg agcgtctgat ggaaggcgct aaactgctga aatgttcaga
     1861 atttggtgat gcgatcatca agaacatgta atcactacat gtgtttaata ttgcaacggg
     1921 cgtataacac gcccgttgtt ttatttatgt aggtattatt aatagcatat cgagcatatt
     1981 tatataaaac cctttacttg atcccatatg ggcatatttt tataatgcaa ctattatgca
     2041 aacatttatt tgttattttg ctttctcctg gaggacactc ttgactgctt ttgagtagac
     2101 tccataaatc cttgttgaat ggtgcgatgt tataaatagt aataggatat tctttatctt
     2161 taaggataat accagattta accggtgtaa atatactgcc aggagggaga aatatagtag
     2221 attgatacca gatgatcatt ttcatattac cccatatggc tgaaaaagat atgccgcatg
     2281 aaggttgaat tatcgtgtca attactatcc acttcatttg ttatg
//
LOCUS       DAZKHC020000266         2300 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZKHC020000266 DAZKHC020000000
VERSION     DAZKHC020000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.267"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(525..959)
                     /gene="uspF"
                     /locus_tag="ACHOSM_RS24985"
                     /old_locus_tag="pgaptmp_004997"
     CDS             complement(525..959)
                     /gene="uspF"
                     /locus_tag="ACHOSM_RS24985"
                     /old_locus_tag="pgaptmp_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HGU4561287.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPVHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(1100..2233)
                     /gene="ompN"
                     /locus_tag="ACHOSM_RS24990"
                     /old_locus_tag="pgaptmp_004998"
     CDS             complement(1100..2233)
                     /gene="ompN"
                     /locus_tag="ACHOSM_RS24990"
                     /old_locus_tag="pgaptmp_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HGU4561288.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT          634 a          493 c          528 g          645 t
ORIGIN      
        1 gtcgtatgca agaacgtgcc gcggttggct ggtaagcttt tcgatagtgc gagtatctaa
       61 tgatttccag ccggaactga ttctattgga aagaaatcat gggcgcaatt tacagatcac
      121 agattgatta gttatagatt tttactgtgg caaaaatggg acaaaaggcc gcaaaagggg
      181 caaaaatggg gcaaaaaaag tgtagattat tgtgctttat tgtagttgtt aatcctgcct
      241 aacatattga aaaacataaa agttattgtt cgtcagatag ttgcggtttt tactctgact
      301 tgttcaggtt gtattgttct ttcttactaa tttcttgatt ttgcgacatt taaaagcgac
      361 tcaattcgtt atatggcatc agaagagtat gcgtcatgcc ggaatgccca gcataagaaa
      421 tctgatataa aaactgtggc gtgtatggta cggattagag gggaaaatgt cagcacattt
      481 gcgaaatgaa tcaaaaagcc cgcagcaatg tgcgggcgtt attgtcagcg cacaaccagc
      541 acggagcatt ctgcgtgacg cacgacagct gcggcgttag aaccgagcag ataagtggtg
      601 atatccggtc gatgggaagc aatgatgatc atatgaacgg ggatcttctt cgccatttcc
      661 agaatgcggt ctttgggcga gccttcctca acatggacat gcactctgtc ggttggcagt
      721 ttaaattttt taatgatctc ttccagttgc gatttggctt ccgctttcag gtcatccatt
      781 gccggtaatt ctgcggaata cgctaaaccc agagaggcat agtagggcag tgaaggtatt
      841 accgtcagga aatgaacctc tgcatcatca atctttgcct ctgcctcaac gtggctaatc
      901 acgcgttgag ttaattctga atcggaaata tcgataggga caagaatcgt tctgttcata
      961 aaacctcctg ttttagtatc cgcataaagt gtaacgccag atgacacttt ttgtgtaatg
     1021 acggagttca catttttaat ttagatcaaa ggaggaagaa taagcagaaa aagcccgcca
     1081 taacagcggg caggaggatt tagaactgat aaaccagacc taaagcgaca atatcatcgg
     1141 tagagatgcc attggcagcg tagaaactgt catcttcatc caacaggttg attttatagt
     1201 caacataagt ggacatgttt ttattgaaat agtaagtcgc gccaacatcg gcgtatttaa
     1261 ccagatcttt atcatcaaca cctgccgggt tgtctgcacc accggcagcg tgcaggtcac
     1321 ggcctttaga catcaggaaa gagactgccg gacgcagacc aaaatcaaac tggtactgtg
     1381 cagtgacttc aaagttctgg gttttgtttg caacagcata atcgctgtcg ccaaacgggg
     1441 tcatattacg cgtttctgaa tacatggttg ccaggtaaat attgttagca tcgtatttta
     1501 gcccagcagt ccacgcgtct gctttatcac cacccgccgc agtatggtta acctgatcat
     1561 tggtgcggtc agaagaggtg tatgccgcac cagcgctaaa gcccatgcct aaatcatatg
     1621 ttgtggaaag accccagccg tcaccgtttt catggcgaac atcacgtccg ttgttggtgc
     1681 cttcctgacc attactggca ccttcgttgt taccttgata ctgcaccgcg aagttcagac
     1741 catttaccag accgaagaaa tcagtattac gataagtcgc gacgccgttg gcacgaccag
     1801 tcataaagtt gtctgcattg gtataagagt caccgccaaa ttcaggcagc atatcggtcc
     1861 agccttcgat gtcgtacatt acgccataat tacgtccgta atcgaaagaa ccgtaatctg
     1921 caaatttcag cccggcaaat gccagacggg tccatgactg gttttttgaa gattcagtgt
     1981 tgtttgcctg aatattgtat tcccattgac cgtagccagt gagttgatcg ttaatttggg
     2041 tttcgccttt aaaacccaga cgcgcatagc tctgatcgcc atctttcgct gaattatcag
     2101 aaaaataatg caggccatca actttgccat acagatctaa tttgttgccg tctttattat
     2161 aaacttcggc tgcatgtgca gcacctgcgg cgagcagggc aggaattaaa agtgccagta
     2221 ctttgctttt cattgaataa atcctttagt tattttattt gccttttatc cccattttgg
     2281 ggagtgaaaa catcctaaag
//
LOCUS       DAZKHC020000267         2276 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZKHC020000267 DAZKHC020000000
VERSION     DAZKHC020000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.268"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..1284
                     /locus_tag="ACHOSM_RS24995"
                     /old_locus_tag="pgaptmp_004999"
     CDS             61..1284
                     /locus_tag="ACHOSM_RS24995"
                     /old_locus_tag="pgaptmp_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4561289.1"
                     /translation="MALSDAWLRSVVGKERDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELESNRNPRLVKLAEKRKATEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGNIPHDAATLHDWLEVLEPLSTKTPA
                     IADRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEIKILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITAKVDDFDFINKIWTVPPERHKRGDITGEPL
                     KRPIIELVEELIKYVISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQYAWRRLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYQAWW
                     ERVESIVTCTRLGSK"
     gene            1467..>2276
                     /locus_tag="ACHOSM_RS25000"
                     /old_locus_tag="pgaptmp_005000"
     CDS             1467..>2276
                     /locus_tag="ACHOSM_RS25000"
                     /old_locus_tag="pgaptmp_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HGU4561290.1"
                     /translation="MQFITNGPDIPDELLQAHEEGRVVFFCGAGISYPAGLPGFKGLV
                     ELIYQRNGTTLSEIEREVFERGQFDGTLDLLERRLPGQRIAVRRALEKALKPKFRRRG
                     AIDTQAALLRLARSREGALRLVTTNFDRLFHVAAKRTGQAFQAYVAPMLPIPKNSRWD
                     GLVYLHGLLPEKADDTALNRLVVTSGDFGLAYLTERWAARFVSELFRNYVVCFVGYSI
                     NDPVLRYMMDALAADRRLGEVTPQVWALGECEPGQEHRKAIEWEAKGVTPIL"
BASE COUNT          598 a          472 c          604 g          602 t
ORIGIN      
        1 tgtgcttgta cataatgtac aaaacagcag aggttttttg ttatttgtac aaggtgagta
       61 atggcgctgt ctgatgcgtg gttgcgttca gtcgttggaa aggaacgtga taaggttttg
      121 gttaaatccg atcgtgatgg tctgtctgtt agagtatcac cgaaaggtcg cgtagtgttc
      181 caatatcgtt atcagtgggc ggggaaaggt gagcgtcttg atatcggaac ttacccggca
      241 actggactaa aagaggccag agaagaagtt atccgtcttc gtggtgaact cgagtcaaac
      301 cgcaatccac gattggtcaa gctggcagaa aaacgtaaag ctactgaagc catgacggta
      361 gaatctgtga tccgtgcttg gtatgaagca tattgtgtaa aaaataaaaa aggttctgaa
      421 cagatactcc gctcgtttga gctgcacctg ttctctaaaa tcgggaatat ccctcacgat
      481 gcagctacat tgcatgattg gttagaagtc ctggagcctc ttagcactaa gactccagca
      541 atagcagacc gattgctaat taacgcaaag caggcccatg tctgggcgta taagagaaag
      601 ctcattgaaa ctcgcccgct gtcggatatc acgggtaaag atatggatat ccgtaaaggt
      661 cagaagaaac ggtttctgac acatgatgaa attaaaatcc tttatgctgc gatcgatggt
      721 tctcgaatgg ttcctaaata ccgggccttc attaaactat tgctgcattt tggttgccgt
      781 agttcagagc taattactgc caaggtggac gattttgatt tcattaataa aatatggact
      841 gtaccaccag aacgacataa gagaggggat ataacaggcg aaccgctaaa gcggcccatt
      901 attgaactgg ttgaagagct tataaaatac gttatctcta tgaacaacgg ttccgatatg
      961 ctttttacta aggaaggaag cagggaacca gttggtcgga catcattgca gtcgctgcct
     1021 tacaatttaa tgcagtacgc atggcggcgt ttggggtatc aatttcctca ttggtctctt
     1081 catgatttga gacgaacagc acgaacaaac ttttctgatc ttactgcgcc tcatattgct
     1141 gaaataatgc tcggtcataa actgccaggt gtatggcaag tttatgacaa gagtgattat
     1201 ctagaagaac agcgtaaagc ctaccaggca tggtgggaga gagttgaatc gattgttact
     1261 tgtactcgtt taggctcgaa atgatatttt gcgtagctag aacgcaatca aatctagcag
     1321 tccgctttgt tcggagttcg gacattatga gttggcaagt aaagtagctt gctaggaagc
     1381 cggatttgca cggtcggtat aataagatgt aaccccttgc cttcatttac tcgaatgaac
     1441 gtgcacattg gataggagga aaaggaatgc aattcattac caacggccct gatattcctg
     1501 atgagctttt gcaggcgcac gaggaagggc gcgttgtgtt cttctgtgga gcaggcattt
     1561 cctaccctgc tggtttacct ggtttcaaag ggttggtaga actaatttac cagaggaacg
     1621 gaacaacact ttcagaaatt gagcgtgagg ttttcgagcg tgggcaattt gacggcacat
     1681 tagatttgct ggaacggcgc ttaccagggc agcgtatagc cgtccgacgc gcgttggaaa
     1741 aagcccttaa gccaaagttc cgtcgtaggg gcgctattga tactcaggcg gcgctgttac
     1801 gtttagcccg tagccgcgag ggtgcccttc gattggtcac taccaacttt gaccgtctct
     1861 ttcatgtggc agctaaacgt acaggccagg cttttcaggc ctatgtagcg ccgatgctgc
     1921 caattccaaa aaacagccgc tgggatggac ttgtatacct gcatgggctg ttaccggaaa
     1981 aggcggatga tactgccctg aatcgtctgg ttgttaccag cggtgacttt ggcttggctt
     2041 atctcactga gcgttgggca gctcgctttg tgagtgagtt atttcgtaac tatgtggtct
     2101 gcttcgttgg ctacagcatc aacgacccgg tactgcgcta catgatggat gcgcttgcag
     2161 cagatcggag gctcggtgaa gtcacaccac aagtatgggc actgggggag tgtgagccgg
     2221 ggcaggagca ccggaaagcc atcgagtggg aggccaaagg ggtcactcct atcctt
//
LOCUS       DAZKHC020000268         2230 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZKHC020000268 DAZKHC020000000
VERSION     DAZKHC020000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.269"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1010)
                     /gene="escU"
                     /locus_tag="ACHOSM_RS25005"
                     /old_locus_tag="pgaptmp_005001"
     CDS             complement(<1..1010)
                     /gene="escU"
                     /locus_tag="ACHOSM_RS25005"
                     /old_locus_tag="pgaptmp_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HGU4561291.1"
                     /translation="MSEKTEKPTPKKLRDLKKKGDVTKSEEVMAAVQSLILFSFFSLY
                     GASFFVEVVELVNTTIDSLNRPFLYAIREILGAVLNIFLLYILPISLIVFVGTVTTGV
                     SQTGFIFAVEKIKPSAQKISVKNNLKNIFSVKSIFELLKSVFKLVIITLIFYFMGHSY
                     ANEFANFTRLNAYQTLVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKHEGLKKMKMSKD
                     EVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTHIAICLYYKLGE
                     TPLPLVIETGKDAKALQIIKLAELYDIPVIEDIPLARSLYKNIHKGQYITEDFFEPVA
                     QL"
     gene            complement(1003..1779)
                     /gene="escT"
                     /locus_tag="ACHOSM_RS25010"
                     /old_locus_tag="pgaptmp_005002"
     CDS             complement(1003..1779)
                     /gene="escT"
                     /locus_tag="ACHOSM_RS25010"
                     /old_locus_tag="pgaptmp_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HGU4561292.1"
                     /translation="MNEIMTVIVSSFFCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIVLKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFITVIFVIHGGIQSVLDKLYLSYEILPLQAD
                     IAFNRALVDFLFSLWDSFIKLMLSFSIPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLP
                     VKSLIAIFILLLVIHVFPDFITANIHSDIIDRSLPSMINE"
     gene            complement(1779..2048)
                     /gene="escS"
                     /locus_tag="ACHOSM_RS25015"
                     /old_locus_tag="pgaptmp_005003"
     CDS             complement(1779..2048)
                     /gene="escS"
                     /locus_tag="ACHOSM_RS25015"
                     /old_locus_tag="pgaptmp_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HGU4561293.1"
                     /translation="METGYFVQLCVQTLWIIFILSLPTVISASVVGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            complement(2048..>2230)
                     /gene="escR"
                     /locus_tag="ACHOSM_RS25020"
                     /old_locus_tag="pgaptmp_005004"
     CDS             complement(2048..>2230)
                     /gene="escR"
                     /locus_tag="ACHOSM_RS25020"
                     /old_locus_tag="pgaptmp_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HGU4561294.1"
                     /translation="KIGFLLYLPFIAIDLIISNILLALGMMMVSPVTISIPFKILLFI
                     LVGGWQKLFEFLLVVN"
BASE COUNT          790 a          416 c          359 g          665 t
ORIGIN      
        1 atcaattgtg ctacaggttc aaaaaagtct tctgttatgt attgtccttt atgtatgttc
       61 ttataaagac ttcttgccaa aggaatatct tcaatcactg gaatatcata gagttcagcc
      121 agtttaatga tctgtagagc tttggcatct tttcctgttt caataactaa aggcaatgga
      181 gtctccccga gtttatagta taggcaaatc gcaatgtgag tcgggttttt gacgatcacg
      241 gttgattttt tgatgttatt agccagactt ccactttgta tctcagaatg aaggcgacgg
      301 cgctcccctt taatttcagg gttaccatca gtatccttgg cttctcgttt cacctcatct
      361 ttactcattt tcattttctt cagtccctca tgcttctgga accagaaatc aaatactgaa
      421 aagagtagat atccgaataa cacgcctttc cataaaagaa aaacaaaaaa ggcaacaacg
      481 acaagagttt gatatgcgtt cagtcttgtg aaatttgcaa actcatttgc atatgaatgc
      541 cccataaagt aaaaaatgag agtaattatc actaacttaa atactgactt aagcagctca
      601 aaaatactct ttacagaaaa aatatttttc aggttatttt ttacactaat cttctgagcc
      661 gatggtttta ttttttcaac cgcaaagatg aatcctgttt gtgatacacc agttgtaaca
      721 gttccaacaa agacaatcaa agaaattggc aaaatataca ataaaaatat gtttaatacc
      781 gcacctaata tttcacgaat ggcatacaaa aacggtctat ttagcgagtc tatagtcgta
      841 ttaactaact caacaacctc aacgaaaaaa ctcgcaccat ataaagaaaa aaatgaaaat
      901 aagattaatg actgcactgc agccattacc tcttcacttt ttgttacatc gcccttcttt
      961 tttagatccc ttagtttttt gggtgtgggc ttttctgttt tttcactcat taatcatgct
     1021 cggtaacgat ctgtcaatta tgtcagaatg aatatttgcc gtaataaaat cagggaaaac
     1081 atgaataact aataacaata taaagattgc tatcaaactt tttaccggca gtgataatgt
     1141 gaatacgttt agctgaggcg ctgttttatt gagaaaccca aaccccatat cacataagaa
     1201 gataccgata atcatgggaa ttgaaaatga taacatcagt ttaataaatg aatcccatag
     1261 cgaaaataaa aaatctacta aagcacgatt gaatgcaata tcggcttgta atggtaatat
     1321 ctcgtaggat aaatacaact tatccagaac gctttgtatc ccaccatgaa taacaaagat
     1381 cacagtgata aattggtata aaataacacc agtgatagaa gatgaatcac ttatagacgg
     1441 gttaaaaatt gaagatattg ttgaacctct taaagtatca ataatctgcc cagccgcatc
     1501 tattgcccaa aaaagaatag taaatgataa cccaatgaaa aagcctatac ttatttcttt
     1561 tagcactatg cctgtcaact gaaaaattcc ggatggtaat ttttccgaaa atgtatagtt
     1621 ctctactata attggaagtg taaacgcaat cataattgag ttgcgtataa agttactgag
     1681 caaaacacct gttgaaaata ttggtagaat aataaacatc cccagcggcc tgagtataca
     1741 aaaaaatgat gatactatga ccgtcattat ttcattcatt agccattcac cttcggaatc
     1801 atttcaaata ttatcgacga gaagttgatg attgttgttc ccatccagtg ataagtcagg
     1861 gcaagcgtag caaacactgc tattattttt agcaaaaaag gcaatgtttg atcctgtaac
     1921 tgcgttatag cctgaaccag gctaataata atgccgacaa cagaggccga tatgactgta
     1981 ggcagtgaga ggataaatat tatccaaagc gtttgcacac ataattgaac aaaatatcca
     2041 gtttccatca attcaccacc aacagaaatt cgaataattt ttgccagcct ccaacaagaa
     2101 tgaataacaa gatcttaaaa ggaatcgaaa ttgttactgg cgataccatc atcataccca
     2161 aggccaataa gatattggaa atgatcaaat ctatcgcaat aaaaggtaaa tagagcaaaa
     2221 aacctatctt
//
LOCUS       DAZKHC020000269         2222 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZKHC020000269 DAZKHC020000000
VERSION     DAZKHC020000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.270"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..155
                     /locus_tag="ACHOSM_RS25025"
                     /old_locus_tag="pgaptmp_005005"
     CDS             <1..155
                     /locus_tag="ACHOSM_RS25025"
                     /old_locus_tag="pgaptmp_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561295.1"
                     /translation="ICQWQVIKKRPRHAQPELTRKMIEGNNISDVRMAPPAKFCNDQT
                     ELATCP"
     gene            complement(236..394)
                     /locus_tag="ACHOSM_RS25030"
                     /old_locus_tag="pgaptmp_005006"
     CDS             complement(236..394)
                     /locus_tag="ACHOSM_RS25030"
                     /old_locus_tag="pgaptmp_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HGU4561296.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(480..1223)
                     /locus_tag="ACHOSM_RS25035"
                     /old_locus_tag="pgaptmp_005007"
     CDS             complement(480..1223)
                     /locus_tag="ACHOSM_RS25035"
                     /old_locus_tag="pgaptmp_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561297.1"
                     /translation="MLSSCIVVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
     gene            complement(1476..2099)
                     /locus_tag="ACHOSM_RS25040"
                     /old_locus_tag="pgaptmp_005008"
     CDS             complement(1476..2099)
                     /locus_tag="ACHOSM_RS25040"
                     /old_locus_tag="pgaptmp_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4561298.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAVFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            complement(2096..>2222)
                     /locus_tag="ACHOSM_RS25045"
                     /old_locus_tag="pgaptmp_005009"
     CDS             complement(2096..>2222)
                     /locus_tag="ACHOSM_RS25045"
                     /old_locus_tag="pgaptmp_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HGU4561299.1"
                     /translation="TFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQGEGA"
BASE COUNT          709 a          540 c          407 g          566 t
ORIGIN      
        1 ccatctgcca gtggcaggtc ataaaaaaaa ggccgcgcca tgcgcagcca gaactcacaa
       61 ggaaaatgat agaaggaaat aacattagtg atgtacgcat ggcgcctccc gctaagttct
      121 gcaatgatca aacagaactc gctacgtgcc cttaaaactc gatcatttag cccctccaag
      181 gaggattcac catgcggttg gttttttaat aaacagtaaa caaaaaagtc aagaattatt
      241 cattctgttc tttcatcatc ggccacagca ataccacaat gccgcagacc agagcgccat
      301 cagtcagtac caacattatc ctgctggtga aatccatcat caccatcact aaaagcagga
      361 tcacaacagc aagcagacac agtttataaa acaatgttca gaaaacgcat tcagcatgcc
      421 taaggttcta ttcctacgaa tagccaactt gcaacttaaa atattattta tgcagccaat
      481 taaattctgg tccttacaat atcaacctga agattcttat cttgtgctga ttgataaatg
      541 acaaaccttt tactacctgc attgaaagaa gtagacaaaa ccagacaatt atcataacga
      601 gcaagaacat aataccaacc atcattataa ttaatcattt catattcttt cttaaactgt
      661 ggtttgtaat atcctgtcag aaatgaaaaa agccagaaat atgccacaaa agcaatcatc
      721 acaatctcaa aaaaatgttt ttttataaat ggcttatcat agaagcatga taccgataaa
      781 aatcgcccat aagatcttat cgaaattgta accgccagcg caatcgctgc tgacagtagc
      841 aaaagaggta cctgaatctt ctgtctcaat atagaaaact caataattgc cggcacaaac
      901 aataattcca cagcaaaata aaggcgaaat acatttagct cttgcataga atgttttctt
      961 ttcactgcga aaaagaatac aacaccaata ccccaaccga taagaaatat agcaatgacg
     1021 ataactgcaa aaaataaact tctggcaaca tcatcaacac ctgcacctac aatccaccat
     1081 gggaagccgt agtaaaaaga agtaccccat ccatagaaat aagcactccc ccatccaagg
     1141 catcccatgt aggcaataaa aagtgaagaa ctcctgagca gcgcaccatc cttcataacc
     1201 accacaatac aagatgataa cattggctta caactcataa caaaagcaat tcaatgccgt
     1261 caagaggtta caggctaaaa aaactctatt acattgcagt cagcatgttt actacacaaa
     1321 tacaattcag agcataaaaa ctactcggcg gcaggttatt gagactcatc aatgacatgt
     1381 aaaaaacgcc cattattggt gtcaagtttc cccaaagtta ttcaaaaagt caatattatg
     1441 ccgttaatat gttgccatcc gtggcaatca tggcgctaac gtgtgatcgc attcaaaatg
     1501 ttgtctgcga ttgactcttc cttgtggcat tgcacaacca gagcgtcata cagcggctta
     1561 acagtgcgtg accaggtggg ttgggtaagg tttgggatta gcatcgtcac agcgcgatat
     1621 gcggcgcttg ctggcattct tgaataaccg acgcctttac atcttccgca ctctttctca
     1681 gcaactatcc cccactgctc tgttttggct atatcaaccg cacggcctgt accgtggcaa
     1741 tctctgcatc ttgcgcccgg cgtagcggca ctacggcaat aatccgcata agcgaatgtt
     1801 gcgagcactt gcagtacctt tgccttagta tttccttcaa gctttgccac gccacggtat
     1861 ttccccgata ccttgtgtgc aaattgcatc agatagttga tagccttttg tttgtcgttc
     1921 tggctgagtt cgtgcttacc acagaataca gccattccga atccggcttg tgattgcgcc
     1981 atccccatag cagccatcac atcagtaccg gaaagagagt cagaagccgt ggcccgtggt
     2041 gagtcgctca tcatcgggct ttttggcgaa tgaaatttag ctacgctttc gagtctcatg
     2101 cgccttctcc ctgtacctga atcaatgtga ggtttccgca gaacactgcg ccagtatcga
     2161 tatacatctg gttggcaaat ttgagtggtt tcactgctgg cgtatgacca aagatgaacg
     2221 tg
//
LOCUS       DAZKHC020000270         2162 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZKHC020000270 DAZKHC020000000
VERSION     DAZKHC020000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.271"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..104)
                     /locus_tag="ACHOSM_RS25050"
                     /old_locus_tag="pgaptmp_005010"
     CDS             complement(<1..104)
                     /locus_tag="ACHOSM_RS25050"
                     /old_locus_tag="pgaptmp_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561300.1"
                     /translation="MLKQQDMTETARVVFDELSVTEPATVGEIAQNTYL"
     gene            complement(391..1209)
                     /locus_tag="ACHOSM_RS25055"
                     /old_locus_tag="pgaptmp_005011"
     CDS             complement(391..1209)
                     /locus_tag="ACHOSM_RS25055"
                     /old_locus_tag="pgaptmp_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGU4561301.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALSFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
     gene            complement(1361..1732)
                     /locus_tag="ACHOSM_RS25060"
                     /old_locus_tag="pgaptmp_005012"
     CDS             complement(1361..1732)
                     /locus_tag="ACHOSM_RS25060"
                     /old_locus_tag="pgaptmp_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4561302.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     QCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(1722..2093)
                     /locus_tag="ACHOSM_RS25065"
                     /old_locus_tag="pgaptmp_005013"
     CDS             complement(1722..2093)
                     /locus_tag="ACHOSM_RS25065"
                     /old_locus_tag="pgaptmp_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4561303.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
BASE COUNT          734 a          541 c          357 g          530 t
ORIGIN      
        1 aggtaagtat tctgcgcaat ctccccgact gtcgccggtt cggtgacgct taattcatca
       61 aacacaactc tggcggtttc tgtcatatcc tgctgtttca gcatgtcttt ttaccctttc
      121 cggttaacgt gacacaccaa taactcttgt cgaaaaagcc agcaagctga aagacctgta
      181 ttaataacta ccagcacatt taacgcactg cgctactttg cgggcacaaa aaacccgctc
      241 agaggcgggg tcaagctatg cggcgaaata accactctta acagcatacc tgatttttta
      301 cgtacgtaaa tgctttgccg tgcatatttt tcatgcaaat gtcacgtcct gctatttttc
      361 agtcttataa atttaaaacc atagaaaaaa tcaattatgt tttaaaaatg gataggtaaa
      421 gaataacaag tgacacagat tcaaaccaaa atggaaaagg gtggcaaccc acaaacgccc
      481 actccacatc catgacaatc catacacaac accagataac gtggcaaata atacaagtaa
      541 agcaccacct gaatagtgat aaaaaccaaa caacaaagcc gccacaatta atgcaaccaa
      601 tggagacgtt acttctgata gccgtgattg aatataccct ctaaataatg attcctctgc
      661 cagagacaca aaaaacaaat tagccaatat aaactctggc aaccactcag gaaaatgaat
      721 ctctggcttt aatccaccaa aaaaaacagc caaaaacagg ataagaggaa cagagagcga
      781 cagagccccc cacttccaca aagacacttc tgattttact tcttttttaa acaaagaaga
      841 tgtacacagg actaacaaaa atggcaccag cgctttatca aaattaaaat acattgtata
      901 gggagtactt tgagggccaa cagtaacaga atttagcact acaggattgt gaaaccctgg
      961 ccatagatgg aaagataatg ctatggctga taaaactatg ccaacttcat atatagattt
     1021 agcccaggcg ttatatttcc agttgaactt caaaataata aaaaaaacga ttgtagcaac
     1081 aaaaaataac accgaccaat caataatatc attaagcaca gccaggacaa cagacaccgt
     1141 caacaatgaa aaagccacta ccttatgcca actgagagtt gacagtgaca gtactaacac
     1201 aatccacata agcactcctt ttatttaatg atgaagattg attatcaata ttttcaattc
     1261 accaggcaac attttatcta ccttccacaa tacatgacca tcaaaaaata aacatgttaa
     1321 ttataaacac agaaaacata accctcatca ctatatatcc ctaccgcata tccatatcta
     1381 accggacatt cagagccata agcattcctt caataatacc ttccgcttta taaagccttt
     1441 taccaataag cccatcagaa catctatgct tacgtgcaag agccataaat gtcattccac
     1501 ctacgtaata atccaccaac aaatcgtgca aatactgatt attcttgttt aatcgtgcca
     1561 tacaaccaca aattatcatg gcgtcatcat cagaacactg aggacgtgat ttcactttag
     1621 tcgggattaa tcctttaaaa ccagcagcta tcgagggcca tgttacatct tcatgattat
     1681 ttgctgccca tgccccccat cgctcaagaa ccatctggat atcacgcgcc atcgttacca
     1741 cctctgattt cgtaaatctt cacgcccagc cgtccacctg gcacaggctg accgcgcaca
     1801 atattgattt catcaaactg ctcgtcatca atgagcactt ccgcatgcgt cagcgcatcc
     1861 agcggtgctt tcagaatgtt gtccaggtca cggcggcgct tatccggtgg ttctgcaata
     1921 atttttattg ccagccgtcc ggacaggctt aatttcagcc gctgctggcg aacaataagc
     1981 gccacagccc ggcgataacg ctcaccggct tttgatacaa aatatgtgct gccacgacgt
     2041 cgccagtagg tgttcaccgt cggcgggtaa ggcaaaacaa actctatacg catcagtaac
     2101 ctcttttacc cgagcacgcc ggttgcaaaa gcgtgatcaa gaaaacgaaa aattaaatca
     2161 ac
//
LOCUS       DAZKHC020000271         2139 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZKHC020000271 DAZKHC020000000
VERSION     DAZKHC020000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.272"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..363
                     /locus_tag="ACHOSM_RS25070"
                     /old_locus_tag="pgaptmp_005014"
     CDS             <1..363
                     /locus_tag="ACHOSM_RS25070"
                     /old_locus_tag="pgaptmp_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HGU4561304.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIAQQNSFKHT"
     gene            519..2054
                     /gene="gadC"
                     /locus_tag="ACHOSM_RS25075"
                     /old_locus_tag="pgaptmp_005015"
     CDS             519..2054
                     /gene="gadC"
                     /locus_tag="ACHOSM_RS25075"
                     /old_locus_tag="pgaptmp_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HGU4561305.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
BASE COUNT          482 a          519 c          530 g          608 t
ORIGIN      
        1 taccaggttg ccgcttatct ggcggatgaa atcgccaaac tggggccgta tgagttcatc
       61 tgtacgggtc gcccggacga aggcatcccg gcggtttgct tcaaactgaa agatggtgaa
      121 gatccgggat acaccctgta cgacctctct gaacgtctgc gtctgcgcgg ctggcaggtt
      181 ccggccttca ctctcggcgg tgaagccacc gacatcgtgg tgatgcgcat tatgtgtcgt
      241 cgcggcttcg aaatggactt tgctgaactg ttgctggaag actacaaagc ctccctgaaa
      301 tatctcagcg atcacccgaa actgcagggt attgcccagc agaacagctt taaacatacc
      361 tgataacgtt taacggtaac ggtgtcccga aacgaacccg tttcgggaca atttccaaag
      421 tctgttcact ggcattagca acggaaaata ttgttctgaa tacgcttcag aacaaaacag
      481 gtgcggttcc gacaggaata ccgttttagg gggataatat ggctacatca gtacagacag
      541 gtaaagctaa gcagctcaca ttactcggat tctttgccat aacggcatcg atggtaatgg
      601 ctgtttatga ataccctacc ttcgcaacat cgggcttttc attagtcttc ttcctgctat
      661 taggcgggat tttatggttt attcccgtgg gactttgtgc tgcggaaatg gccaccgtcg
      721 acggctggga agaaggtggt gtcttcgcct gggtatcaaa tactctgggg ccgagatggg
      781 gatttgcagc gatctcattt ggctatctgc aaatcgccat tggttttatt ccgatgctct
      841 atttcgtgtt aggggcactc tcctacatcc tgaaatggcc agcgctgaat gaagacccca
      901 ttaccaaaac tattgcagca ctcatcattc tttgggcgct ggcattaacg cagtttggtg
      961 gcacgaaata cacggcgcga attgctaaag ttggcttctt cgccggtatc ctgttacctg
     1021 catttatttt gatcgcatta gcggctattt atctgcactc cggtgccccc gttgctatcg
     1081 aaatggattc gaagaccttc ttccctgact tctctaaagt gggcacactg gttgtatttg
     1141 ttgccttcat tttgagttat atgggcgtag aagcttccgc aactcacgtc aatgaaatga
     1201 gcaaccccgg gcgcgactat ccactggcta tgttactgct gatggtggcg gcaatctgct
     1261 taagctctgt tggcggttta tctattgcga tggtcattcc gggtaatgaa atcaacctct
     1321 ccgcaggggt aatgcaaacc tttaccgttc tgatgtccca tgtggcaccg gaaattgagt
     1381 ggacggttcg cgtgatctcc gcactgctgt tgctgggtgt tctggcggaa atcgcctcct
     1441 ggattgttgg tccttctcgc gggatgtatg tcacagcgca gaaaaacctg ctgccagcgg
     1501 cattcgctaa aatgaacaaa aatggcgtac cggtaacgct ggtcatttcg cagctggtga
     1561 ttacttctat cgcgttgatc atcctcacca ataccggtgg cggtaacaac atgtccttcc
     1621 tgatcgcact ggcgctgacg gtggtgattt atctgtgtgc ttatttcatg ctgtttattg
     1681 gctacattgt gttggttctt aaacatcctg acttaaaacg cacatttaat atccctggtg
     1741 gtaaaggggt gaaactggtc gtggcaattg tcggtctgct gacttcaatt atggcgttta
     1801 ttgtttcctt cctgccgccg gataacatcc agggtgactc taccgatatg tatgttgaat
     1861 tactggtcgt tagtttcctg gtggtacttg ccctgccctt tattctctat gctgttcatg
     1921 atcgtaaagg caaagcgaat accggcgtca ctctggagcc aatcaacagt cagaacgcac
     1981 caaaaggtca cttcttcctg cacccgcgtg cacgttcacc acactatatt gtgatgaatg
     2041 acaagaaaca ctaaccatgt ctgaatggtt ataagacaag ggagcgataa ttcatcgctc
     2101 cctttttcgt gcttgctgtc gtattgactt aaccgggat
//
LOCUS       DAZKHC020000272         2137 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZKHC020000272 DAZKHC020000000
VERSION     DAZKHC020000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.273"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1566
                     /locus_tag="ACHOSM_RS25080"
                     /old_locus_tag="pgaptmp_005016"
     CDS             <1..1566
                     /locus_tag="ACHOSM_RS25080"
                     /old_locus_tag="pgaptmp_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU4561306.1"
                     /translation="GLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFSAASGNRKP
                     VVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMTKAAEEGRT
                     LDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAAVASAPVIR
                     VEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAVGAGTTTDP
                     QWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQVSGGAAGWV
                     GEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALAEAVVARLD
                     TDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQPTGAVWLMS
                     STNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAPDIYLADDG
                     GVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAVA
                     VITGVNYGSASGG"
     gene            1611..1832
                     /locus_tag="ACHOSM_RS25085"
                     /old_locus_tag="pgaptmp_005017"
     CDS             1611..1832
                     /locus_tag="ACHOSM_RS25085"
                     /old_locus_tag="pgaptmp_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561307.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVM"
     gene            1778..>2137
                     /locus_tag="ACHOSM_RS25090"
                     /old_locus_tag="pgaptmp_005018"
     CDS             1778..>2137
                     /locus_tag="ACHOSM_RS25090"
                     /old_locus_tag="pgaptmp_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4561308.1"
                     /translation="MWNLLRRTRKNQKSGRDVKEVGWTSLFQAVAEPFAGAWQQGVKA
                     DPESVLSFHAVFSCISLISQDIAKMRLRLMQTDTQGIRREKRQGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNT"
BASE COUNT          456 a          564 c          692 g          425 t
ORIGIN      
        1 ggactgcatt ttctgcgctg ggaactgatg gaggtgtctg ccgtcaccgt gcccgcgaat
       61 gcggaatgca ccatccggac cattaaatct tacgaccgcc cgttttctgc cgcgtccggc
      121 aaccggaaac cggtggtgaa aatcgcatct tctgccggcg ctgcggcaca gtcaacaacc
      181 gtttttcata aggaaaagac cataatgaat attggcgaac agattaaaag ttttgaaaac
      241 aagcgtgcag cgctggcagc ctcccttgag gaggtcatga ccaaagccgc agaggaaggg
      301 cgcacgctgg atgtggagga ggaagagcac tacgacaaca ccgcagcgga aatccgtcag
      361 gtggatgcgc acctgaagcg cctgcgtgaa ctggaagccg gtaaggccgc cacggcgcag
      421 ccggtgaaac aggccggtaa cgggaatgtg gccgcggtgg cttctgcgcc ggtgatccgt
      481 gtggagcaga aactggataa ggggattggc ttcgcccgct ttgccaaatc gctggctgcg
      541 gctaaaggcg tccgatctga agccctggaa gtggcccgtc gtcagtatcc ggatgacagt
      601 cgtctgcatc atgtcctgaa atcggcagtg ggcgcgggga ccaccacgga tccgcagtgg
      661 gcaggcagcc tgtctgaata tcaggaatac gcacaggact ttattgatta cctgcgtccg
      721 cagaccatta tcgggcgatt tggtcagggc gggatccctg cacttcgtca ggtgccgttc
      781 aatatccgtg tgcacgccca ggtgtccggc ggtgctgccg gctgggtggg tgagggtaag
      841 gcaaaacccc tgacgaagtt tgattttgaa tccatcacct tcagtcatgc gaaagtgtcg
      901 gccattgcgg tactgacgga agaattgatc cgtttttcca gtccggctgc tgatgcactg
      961 gtccgtaatg cgctggcgga agcggtggtg gcgcgtctgg atacagactt tgtggacccg
     1021 aaaaaagccg cagtggcaga tgtctccccg gcgtccatca cccatgatgt gaagggcacg
     1081 gcatcaaccg gtaacccgga tgcggatgca gaggcggcgt ttggacagtt tgtggcagca
     1141 aacctgcagc ccaccggtgc ggtctggctg atgtccagca ccaatgccct ggcactgtcc
     1201 atgcgtaaaa atgcgctggg tcagaaggaa tacccggaca tgaccctgct gggtggctcc
     1261 ttccaggggc tgccggtgat tgtctcccag tacgtgggtg accagctggt gctggtgaat
     1321 gccccggata tttatctggc ggatgacggc ggcgtggcag tggatatgtc ccgcgaagca
     1381 tcactggaaa tgcagtctga gccgaccggc gacagtacca cgccgtcccc ggtggagctg
     1441 gtttccatgt tccagacagg cagcgtggcc atccgtgcgg agcgctggat caactggcgt
     1501 cgtcgccgta ccgcggcggt ggcggtgatc accggagtga actacggcag tgcgtccggc
     1561 ggctgagtct gatgaggagg acgggaggcg tgcgcctccc gtaacaggtt atggcaaaga
     1621 tccgatatct gcagggcacg catgatgccc gggccgggga tatccgtgat gtggcacagc
     1681 cgtgtgcgga ggtgctggtt cgcctgggga aggcggagta catcacagtg cgacgtccgg
     1741 caggtcagaa aaagaaacgt gatgcggagc atggcgaatg tggaaccttt tgcggcgaac
     1801 ccgaaaaaac cagaaatcag gacgtgatgt aaaagaggtg ggctggacca gcctgtttca
     1861 ggcggtggct gagccttttg ccggcgcctg gcagcagggc gtgaaagccg atccggaaag
     1921 tgtcctctcc tttcatgcgg tgttttcttg catttcgctg atatcccagg atatcgccaa
     1981 aatgcgactg cgcctgatgc agaccgatac acaggggatc cgccgtgaaa aacggcaggg
     2041 ggatattgcc cgtctctgtc gtcgtcccaa tgcacagcag aatcgtatcc agttttttga
     2101 actgtggctg aacgccaaac tgcgtcacgg caatacg
//
LOCUS       DAZKHC020000273         2130 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZKHC020000273 DAZKHC020000000
VERSION     DAZKHC020000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.274"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            27..233
                     /locus_tag="ACHOSM_RS25095"
                     /old_locus_tag="pgaptmp_005019"
     CDS             27..233
                     /locus_tag="ACHOSM_RS25095"
                     /old_locus_tag="pgaptmp_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4561309.1"
                     /translation="MEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(489..761)
                     /locus_tag="ACHOSM_RS25100"
                     /old_locus_tag="pgaptmp_005020"
     CDS             complement(489..761)
                     /locus_tag="ACHOSM_RS25100"
                     /old_locus_tag="pgaptmp_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561310.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            921..1454
                     /locus_tag="ACHOSM_RS25105"
                     /old_locus_tag="pgaptmp_005021"
     CDS             921..1454
                     /locus_tag="ACHOSM_RS25105"
                     /old_locus_tag="pgaptmp_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4561311.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1675..1788
                     /locus_tag="ACHOSM_RS25110"
                     /old_locus_tag="pgaptmp_005022"
     CDS             1675..1788
                     /locus_tag="ACHOSM_RS25110"
                     /old_locus_tag="pgaptmp_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561312.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            1790..2068
                     /locus_tag="ACHOSM_RS25115"
                     /old_locus_tag="pgaptmp_005023"
                     /pseudo
     CDS             1790..2068
                     /locus_tag="ACHOSM_RS25115"
                     /old_locus_tag="pgaptmp_005023"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            2010..>2130
                     /locus_tag="ACHOSM_RS25120"
                     /old_locus_tag="pgaptmp_005024"
     CDS             2010..>2130
                     /locus_tag="ACHOSM_RS25120"
                     /old_locus_tag="pgaptmp_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561313.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWI"
BASE COUNT          555 a          432 c          587 g          556 t
ORIGIN      
        1 tcggcggtga cggagacata catcagatgg aaaaaatcac aacaggtgtg tcatacacca
       61 cgtcagcggt ggggacggga tactggttac tgcagctgct ggacaaagtc tctccgtccc
      121 agtgggtggc aataggtgtg ctgggaagtc tgctgtttgg cctgctgacg tatctgacta
      181 acctgtattt caaaatcaga gaggaccgtc gtaaggtggc gcggggagag tagtcgatga
      241 ataaacaata cgaactggtt gtaaaatgaa tatttctaac tgaaaaaacg ttccatgagg
      301 taagaaaagg tcacaggcaa tcaataacag gacgtgatga aagacccttg catttgtgcg
      361 ctttctcttt agatagcagc agatactgaa aatctgagtt gtcggggagt cagggataca
      421 gctgtgcaag agttggtcat tgtgattcca ttgaaatcct gtatgccatg aagggcagga
      481 ttttatggct acctgagctt tggtgatagt aagttgaaaa ttcgcatttt ttgctgacat
      541 gcgtaacgag aatcccataa acagggagga cttaattctt cattaaccca tgcgttgata
      601 ttatgtttca gccgttgaag catcagcggt gttaatgttg tggtaataat atccagcgtt
      661 ttatgtgaga tcttaccgta agggtctgca agaatgctgc ttgttgcttc gttattatct
      721 gccatcagaa gaagtaactc tgatttaacg ttttctgtca ttagttgtaa aaatcttctg
      781 cgcaaacttt ctttactgtt catttatatg gcttcatttg ttgtaatctg ctgcgtctca
      841 agggatatgt ttatgagagc gaccatgagt gtttgattat atacctaaca tatcaaggga
      901 ttagaaatcg ataaatcccc atgaacgaaa aaataaaata cggcctgtcg gctgccgttc
      961 tggcgctgat tggtgcaggt gcttctgcgc ctgaaatcct cgaccagttt ctggatgaaa
     1021 aggaaggtaa ccacaccacg gcataccgtg atggtgcggg gatctggacc atctgccgtg
     1081 gtgccattct ggtggatggt aagcctgtta ttcctggcat gaagctgtca aaggaaaaat
     1141 gcgaccgggt taatgccatc gaacgtgaca aggcgctggc atgggtggag aaaaacatcc
     1201 gggtgccgct gaccgaaccc cagaaagcgg ggatcgcgtc attctgtccg tacaacattg
     1261 gccccggtaa gtgcttcccg tcgacgtttt ataaacgaat taatgcaggc gatcgaaaag
     1321 gtgcctgtga ggcgattcgc tggtggatta aggacggtgg cagagactgc cgtatccgtt
     1381 caaataattg ctacggtcag gtctcacggc gtgaccagga gagcgcgctg gcgtgctggg
     1441 acatcgacag atagcagaat attttcctga aaaatgacgt tggccaacgc gggtggataa
     1501 cacgaaatcc tgaaaactgg taaaacctaa gtgaataaaa gtaaaaaccc cgtttgttgg
     1561 cagcaagcgg ggttttgtgt tttctgacct tgagtaaggc aagggagaaa ttatgggtag
     1621 ggaggtactt tccctgtgag gaagtataaa agattctttc tgaggttgtc cattatgaaa
     1681 ggcattgaag tggagacgcc agccagtctg gatttaacaa gagcggcagc ttttgccatt
     1741 cgtattgtgg ccattgctgt tctggtctgg gcaatccgtt ggtggtgata tgaaccgtgt
     1801 tctgtgcgtg gttatcattg tcctgctggt ggcctgtggt gcgcttagtc tggggctgaa
     1861 tcattaccgt gatcacgcca tcatctacaa agagcagcgc gataaaaaag ccagtgagct
     1921 ggagctggcg aacgcgacaa ttactgatat gcagatacgc cagcgtgatg tcgctgcact
     1981 tgatgccaga tactcgaggg aattagccga tgcgagagct gaaaatgaaa ctctgcgtgc
     2041 tgatgttgcc gctggtcgta agcgcctgcg gatcaacgcc acctgctccg gtaccgtgcg
     2101 tgaagccacc ggcacctccg gcgtggataa
//
LOCUS       DAZKHC020000274         2120 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZKHC020000274 DAZKHC020000000
VERSION     DAZKHC020000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.275"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2049)
                     /locus_tag="ACHOSM_RS25125"
                     /old_locus_tag="pgaptmp_005025"
     CDS             complement(<1..2049)
                     /locus_tag="ACHOSM_RS25125"
                     /old_locus_tag="pgaptmp_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU4561314.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPSRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEK
                     R"
     gene            complement(2046..>2120)
                     /locus_tag="ACHOSM_RS25130"
                     /old_locus_tag="pgaptmp_005026"
     CDS             complement(2046..>2120)
                     /locus_tag="ACHOSM_RS25130"
                     /old_locus_tag="pgaptmp_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4561315.1"
                     /translation="QIVVDEIRDTLEKAVIDCCDEADM"
BASE COUNT          453 a          677 c          537 g          453 t
ORIGIN      
        1 gcgtttttct gccccggccg tcagttccgg ggataccgtt tcaccagaac gctcctgcgg
       61 gaagacgagc cacgtttccc gctgtgccca gtccggtgcg gagggccagc ggatcttttc
      121 gtaaccatgc agaacggcaa gcgcatccgc ataaaccagc aggtcaaacg cttcgttagc
      181 gcccctgccc ggttttcgcc attttccgtc actgccgcgc tcttcatagg tcagctcatc
      241 gtaaaaccac cgccccagcc agtcgggaaa gtggatatag ttcggccctg gtgtgtcacg
      301 ccacagggca ttatttacac gatccttaaa cgcatccgtc tgaaccagcc acagcgcgac
      361 atcgccactg gctctggcac ggcgggcact tctgccggta ttatccggga aggttcggtt
      421 aatcagcctg tcacggcgaa gtccatcccc cttgaacaga aacaccctgt tgcccagtcc
      481 gtcactccgg caacgacgcc agaaacgata ggcgttatct gtcaccccgg cttcccctcc
      541 cgtatccacc gccatggcca tcagacgcat gcgcacatcc ggatcagaag ccagcggcca
      601 tgttttatgg aacacatccg tcagcaacaa atcccagtcc tccggatatg ccgccggatc
      661 aaccggcaga ctttcaccgt tgggactgca gcgcagtgaa tgccggatgt tgtagcgatc
      721 aacaatccag cgttccccct gctctccgta tccggtgatc tgcacaacaa aacggcgatt
      781 tttaccgccc tgtacgtcaa ccgttgcctc aataaaacgc acaccatccg gcacagatcg
      841 ccggggaaac ggctcggcac gctgttcaag cagttcactt ttacgctgtt ccgtggctga
      901 acggggcaga tagggtcgtc cgatatcggt gttcaccacc gctttcaggg tctcttcact
      961 gccggttcgc tcatactctt cttctgccgc cagcagttta aaaatcagtt gttcccaggt
     1021 ctgaaacgcc gcagctggcc cctccatcca aaatgacgca atccgggagt ttcgtggcgt
     1081 tccggtgata ctgccgtccg ccgccgcccg ttcaccttca cgaagccaga tcccctggtt
     1141 attcagttcg cgtttctgct caggggcaat cagcccgcga caatgcggac acatcagacg
     1201 ggcagcctga ccggcagcca caaaatccgg gttattccgg tatccggtca tgttatccat
     1261 caccggctga aaatattccc cgcagtgcgg acacggccag taccaccggc ggcggtctcc
     1321 ccggttatac agtgacagga tccccgttgt tggcggtgcc tcatgtgcgc caccacaacg
     1381 ccatttggta tcggtgatat cccgccccgg tgaactctcg accagggtca tccccgagga
     1441 cataaaggtg gtggtacgct ttgaggccag cgtgaaggca tccccttccc cgtccacgtt
     1501 ttcagggaaa cggtcataat ccgtcagcgc cacacgacgg taatccgaag aggaaaagac
     1561 ggtgatcgac ggccagccaa tcttcaggaa ggagccgtca agaaacattt tatcgtggac
     1621 gttgttgtca ttacgggaag gactgaggcg cttgctgacc tccggactgt ggcgaaacgt
     1681 cctggaaaga cgcgttctgg aatgctcacg cgccttcgtc tcagtcatct gcaccaccag
     1741 catatccgcc ggatcacaga tgatgccgta cacaatccag ccatcaatca gcccttcggt
     1801 tttcccggtt cgcgcaggtc ccacaaacac caccgcgtca tattcacggg ctgataatgt
     1861 attaatgggg tcaatcatat agggcgtcag cgatgactcc cacggaccgg aagtattggc
     1921 tccccgtgga acccgcatat aacgcctgat ggcttccgct actggtaacc ggctgggtgg
     1981 gcgaaacagc gaggccactt cgcgccagat atcggatgcg cggctatggc tctcgttcac
     2041 ctgattcaca tatcggcctc atcacaacag tcaatgactg ccttttccag tgtgtcgcgg
     2101 atctcatcaa ccacaatctg
//
LOCUS       DAZKHC020000275         2057 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZKHC020000275 DAZKHC020000000
VERSION     DAZKHC020000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.277"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..246
                     /locus_tag="ACHOSM_RS25135"
                     /old_locus_tag="pgaptmp_005027"
     CDS             <1..246
                     /locus_tag="ACHOSM_RS25135"
                     /old_locus_tag="pgaptmp_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGU4561316.1"
                     /translation="LTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIR
                     WWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            545..1012
                     /locus_tag="ACHOSM_RS25140"
                     /old_locus_tag="pgaptmp_005028"
     CDS             545..1012
                     /locus_tag="ACHOSM_RS25140"
                     /old_locus_tag="pgaptmp_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4561317.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKEQRDKKVSEL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGHRSLRINATCPGP
                     VREATGTARVDNATGPQLADTVTRDYFILRERLMTMQKQLEGAQDYIRTQCLN"
     gene            1454..1999
                     /locus_tag="ACHOSM_RS25145"
                     /old_locus_tag="pgaptmp_005029"
     CDS             1454..1999
                     /locus_tag="ACHOSM_RS25145"
                     /old_locus_tag="pgaptmp_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HGU4561318.1"
                     /translation="MEVNKKQLADIFGSSIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
BASE COUNT          509 a          486 c          581 g          481 t
ORIGIN      
        1 ctgaccgaac cccagaaagc ggggatcgcg tcattctgtc cgtacaacat tggtcccggt
       61 aagtgtttcc cgtcgacgtt ttacagacga attaatgcag gtgatcgaaa aggtgcctgc
      121 gaagcgattc gctggtggat taaggacggt ggcagagact gccgtattcg ttcaaacaac
      181 tgttacggtc aggtatcccg tcgtgaccag gagagcgcgc tggcgtgctg gggaatcgac
      241 agataagaag aatattttgc tgaaaaatga ggtttgctca cacggacgga taacacgaaa
      301 tcctgcgaac tgacaaaaac taagtgaata aaagtaaaaa ccccgtttgt tggctgcaag
      361 cggggttttg tgtttcctga ctctggaaaa gtcaaaggag aaagtgtgtt tgattttagc
      421 aaactgattc gggagattcg agtgatggct gaaaaattat ctacctggaa gttcattctt
      481 atctggcggg tgtttgtgat tatggcctcc ggttatttca tcggtcagat acgctggtgg
      541 tgaaatgaac cgcgtactgt gcgtggtcat cattgccctg ctggtggcct gtggtgcgct
      601 tagtctgggg ctgaatcatt accgtgataa cgccataacc tacaaagagc agcgcgataa
      661 aaaagtcagt gagctggagc tggcaaatgc aaccattact gatatgcagc agcgccagcg
      721 tgatgttgct gcacttgatg ccagatactc gagggaatta gccgatgcga gagctgaaaa
      781 tgaaactctt cgcgctgacg ttgccgctgg tcaccgcagc ctgcggatca acgccacctg
      841 tccaggtccc gtgcgtgaag ccaccggcac cgcccgcgtg gataatgcaa ccggccccca
      901 actggcagac accgttacac gggattattt catcctcaga gaacggttga tgacaatgca
      961 gaagcagctg gaaggggcac aggactatat ccgcactcag tgcctgaact aagttttgct
     1021 gatgcgccgt atcgtcgctg tattccctca ttaacagaga ccgcagcccg acagggagac
     1081 tactctgcgc gagtgtgcgg ggataatcaa aaacgataca caccggggtt taccgcgtaa
     1141 acggagcgcg gcgttgtccc ctcatggtcg ctggtccggt gcgatggtgg aagaaactgg
     1201 atttgctgca actgataacc attatcattt tcgcgggtcc tttccggcga tccgacaggt
     1261 tacggggcgg cgacctcgcg ggttttcgct atttatgaaa attttccggt ttaaggcgtt
     1321 tccgttcttc ttcgccgtaa cctaatgttt ttatttaaaa caccccctga aaagaaagga
     1381 aacgacaggt gctgaaaacg ggctttttgg cctctgtcgt ttcctttctc tgtttttgtc
     1441 cgtggaatga acaatggaag tcaacaaaaa gcagctggct gacattttcg gttcgagtat
     1501 ccgtaccatt cagaactggc aggaacaggg aatgcccgtt ctgcgaggcg gtggcaaggg
     1561 taatgaggtg ctttatgact ctgccgccgt cataaaatgg tatgccgaaa gggatgctga
     1621 aattgagaac gaaaagctgc gccgggaggt tgaagaactg cggcaggcca gcgagacaga
     1681 tctccagcca gggactattg agtacgaacg ccatcgactt acgcgtgcgc aggccgacgc
     1741 acaggagctg aaaaatgcca gagactccgc tgaagtggtg gaaaccgcat tctgtacttt
     1801 cgtgctgtcg cggatcgcag gtgaaattgc cagtattctc gacgggatcc ccctgtcggt
     1861 gcagcggcgt tttccggaac tggaaaaccg acatgttgat ttcctgaaac gggatatcat
     1921 caaagccatg aacaaagcag ccgcgctgga tgaactgata ccggggttgc tgagtgaata
     1981 tatcgaacag tcaggttaac aggctgcggc attttgtccg cgccgggctt cgctcactgt
     2041 tcaggccgga gccacag
//
LOCUS       DAZKHC020000276         2035 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZKHC020000276 DAZKHC020000000
VERSION     DAZKHC020000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.278"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..419)
                     /locus_tag="ACHOSM_RS25150"
                     /old_locus_tag="pgaptmp_005030"
     CDS             complement(<1..419)
                     /locus_tag="ACHOSM_RS25150"
                     /old_locus_tag="pgaptmp_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HGU4561319.1"
                     /translation="MKIISTVIQTPFPFENNNSHTGVVTEPILGKLIGQGSTAEIFED
                     MNDSSALYKKYDLVGNQHNEVLEMARQESALFNTFYGDDASVVIQYGGDVYLRMLRVP
                     GIPLSDIDTADIPDNLESLYLQLICKLNELSIIHYDL"
     gene            complement(595..1292)
                     /locus_tag="ACHOSM_RS25155"
                     /old_locus_tag="pgaptmp_005031"
     CDS             complement(join(595..1043,1043..1292))
                     /locus_tag="ACHOSM_RS25155"
                     /old_locus_tag="pgaptmp_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HGU4561320.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRHRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEV
                     VVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARPGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            complement(1553..1822)
                     /locus_tag="ACHOSM_RS25160"
                     /old_locus_tag="pgaptmp_005032"
     CDS             complement(1553..1822)
                     /locus_tag="ACHOSM_RS25160"
                     /old_locus_tag="pgaptmp_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4561321.1"
                     /translation="MNVLKKLMQRLCGYGKHDDREHGELLTAQLRLGPADILESDENG
                     IIPEQARVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(1824..>2035)
                     /locus_tag="ACHOSM_RS25165"
                     /old_locus_tag="pgaptmp_005033"
     CDS             complement(1824..>2035)
                     /locus_tag="ACHOSM_RS25165"
                     /old_locus_tag="pgaptmp_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4561322.1"
                     /translation="PASIIETNSHGWFPDTDGALITGLTFLDPKDATRVQGFFQHLQV
                     RFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          539 a          527 c          429 g          540 t
ORIGIN      
        1 ttaagatcgt aatggattat actcaactca ttcaatttac atatcaactg cagataaagg
       61 ctctctagat tatcaggaat atcagctgta tcaatatcac taaggggaat ccctggcacg
      121 cgtagcattc ggagatacac atcaccgcca tactgtataa caactgatgc gtcatcgcca
      181 taaaaagtat taaaaagggc agattcttgc ctagccattt ccagaacttc attgtgctgg
      241 ttaccaacaa gatcatactt tttatataaa gcagacgaat cattcatatc ttcaaagatt
      301 tctgctgttg aaccttgccc tattaactta ccgagaatag gctccgttac tacgccagta
      361 tgagaattat tattctcaaa tgggaaaggt gtttgaataa cagtggatat tattttcatg
      421 acatgccctt gttatgaaat ttaaagatta caagatattt ttacataaaa taatattacg
      481 cgctacctcc tcagaaacca cgtaagtaca tatgctatta catagataac cgccatgatc
      541 agggaagccc catacaagca cagtgtagag aggcctttag gtaatgactc caacttactg
      601 atagtgtttt atgttcagat aatgcccgat gaccttgtca tgcagctcca ccgattttga
      661 gaacgacagt gacttccgtc ccggccttgc cagatgttgt ctcagattca gattatgtcg
      721 ctcaatgcgc tgagtgtaac gcttgctgat aacgtgcagc tttcccttca ggcgtgattc
      781 atacagcggc cagccatccg tcatccatac cacgacctca aaggccgaca gcaggctcag
      841 aagacgctcc agtgtggcca gagtgcgttc accgaagacg tgcgccacaa ccgtcctccg
      901 tatcctgtca tacgcgtaaa acagccagcg atgacgtgat ttagcaccga cgtagcccca
      961 ctgttcgtcc atttcagcgc agacaatcac atcactgccc ggttgtatgc gcgaggttac
     1021 cgactgcggc ctgagttttt taagtgacgt aaaaccgtgt tgaggccaac gcccataatg
     1081 cgtgcactgg cgcgacatcc gacgccattc atggccatat caatgatttt ctggtgcgta
     1141 ccgggctgag aggcggtgta agtgaactgt agttgccatg ttttacggca atgagagcag
     1201 agatagcgct gatgtccggc agtgcttttg ccgttacgca ccacgccttc agtagcggag
     1261 caggaaggac atctgatgga aatggaagcc acgcaagcac cttaaaatca ccatcataca
     1321 ctaaatcagt aagttggcag cattaccgag attgttttgt cggaactgat acgctggcgc
     1381 taataggtag tagggttctt atgctaaaca tacaacctct ttcttaattc tagaacctgt
     1441 ttaggattct gtgtaaattc aaaataaacc tgtctgaaca ttcctaacaa acatccacct
     1501 gacatgacaa caaaaaaccg gagccggact ccggttttgt gaagctgtcg ggttacttca
     1561 tcccgccaat attttcccac gtcccgtcag cacgcaggat ttgcagcggt cttaccacac
     1621 actgtatctg ctttttatcc gcatccagta tcaccacctg cgtgattacc ctggcctgct
     1681 ccgggataat accattctca tctgactcca ggatgtctgc cggccccagt cgcagttgtg
     1741 ctgtaagtaa ctccccgtgt tcacggtcat catgctttcc gtacccgcac agacgctgca
     1801 taagtttttt tagtacgttc atgtcattct cctgttctgc ctgtatcact gcccacttca
     1861 tccagcccct tgacatcctg ccacggcccg tcaccaaacc tgacctgcaa atgctgaaaa
     1921 aaaccctgaa cccgtgtggc atctttgggg tcaagaaagg tcagtccggt gatgagtgcg
     1981 ccatctgtat ccgggaacca gccatggctg tttgtctcaa taatgctcgc cggcc
//
LOCUS       DAZKHC020000277         2008 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZKHC020000277 DAZKHC020000000
VERSION     DAZKHC020000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.279"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1123..1494
                     /gene="ler"
                     /locus_tag="ACHOSM_RS25170"
                     /old_locus_tag="pgaptmp_005034"
     CDS             1123..1494
                     /gene="ler"
                     /locus_tag="ACHOSM_RS25170"
                     /old_locus_tag="pgaptmp_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HGU4561323.1"
                     /translation="MNMETNSHTTSPYIQLIEQIEVLQQEAKRLREQEIQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEDEIIPLKNI"
     gene            1509..1727
                     /locus_tag="ACHOSM_RS25175"
                     /old_locus_tag="pgaptmp_005035"
     CDS             1509..1727
                     /locus_tag="ACHOSM_RS25175"
                     /old_locus_tag="pgaptmp_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscE/YscE/SsaE family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HGU4561324.1"
                     /translation="MITITELEDEIIKNKEAANIFIEKINDEKNEIHEKMKHPLDKVT
                     YNEAKELLIACDAAIRIIEIMLIRINNK"
     gene            1740..>2008
                     /locus_tag="ACHOSM_RS25180"
                     /old_locus_tag="pgaptmp_005036"
     CDS             1740..>2008
                     /locus_tag="ACHOSM_RS25180"
                     /old_locus_tag="pgaptmp_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system filament chaperone"
                     /protein_id="HGU4561325.1"
                     /translation="MSQTKNRELLDKKIRSEIEVIKKIIAEFDVVKENVNALSEKAKT
                     DPQAAEKLNKLIEGYTYGEERKLYDSALSKIEKLIETMSPPRSKN"
BASE COUNT          697 a          308 c          410 g          593 t
ORIGIN      
        1 tttatagcga gagttttaaa tgtatagaga gttatttttt taaattagag gaaatactcc
       61 tctaatgacg gacaatagat tttcattgat tcatcagcaa tagtttttgt gcctacgaaa
      121 aggttgtaat tatttacatt aataatagca ttcagaaaaa atttttattg tgctgataaa
      181 atataccatt agtgattagt ttagatacct ggctttcagg caacttcttt ttgtctgtat
      241 ataagggatg attactaagg tggtacattg atatcagtta gctttccctt ttcgggttac
      301 tttatcaaag agtcctcttt atagaagagc gcattgtgag ttttccagat tgacttaaac
      361 gtgtggcggc atcacaccga tcaacacgat ctgcagcaaa gggcaggtgt aaccaggccc
      421 atcttagcgc tatgcacttt tgagccactg agcttgctaa ccgtttaccc ggctttcact
      481 gagctaatta cggcgataat ctgatgccca gtaaaacgga cttgatgtat gacgggtcct
      541 cttttgcggg atagatatgg gaatatttct taatgcgaat ggtacggtta tgcggggggg
      601 acaaacatgg ttgcttgttc tgccatttgt tccttgttcc tttttatacc gccgccgctc
      661 ccggtcagca ctggggaata gggggttgag gtatatctgc tccttatgtc aggggaacag
      721 gtaatagcta tatagcggaa cgtcttagtg ctgtagttat tccattttgc tatttgtcta
      781 ataggactaa tcttaaagaa aacaaaacga ttaactaaat ggaaaggcaa ttgttaatgg
      841 cgtttgctaa agagatgaag ttcttctact ttattgattt tgaatgattt ttaagaaata
      901 taattttttt gttgacattt aatgataatg tgttttacac attatacatc ggtgattaat
      961 aacttttata aggattttgt ttgatgaagt agatgtgttc taatttgata gataaaacgt
     1021 tatctcacat aatttatatc attcgattaa ttgttgatcc ttcctgataa ggataagatt
     1081 gctaatagct taatatatta aagcatgcgg agattattta ttatgaatat ggaaactaat
     1141 tcgcacacaa caagcccata cattcagctt attgagcaaa ttgaagtgtt acaacaggaa
     1201 gcaaagcgac tgcgagagca ggaaattcaa agtgtaattg agtcgattca aaagcagatt
     1261 acttattaca atataaccct acaagagctg ggatatacta atgtgcctga tgatggcctt
     1321 gctcgccgga actcatcgaa aggagtttat tatcgcaatg aagaagggca gacctggtcg
     1381 ggagttggcc gacagccacg ctggcttaaa gaagcactgt tgaatggaat gaagaaagaa
     1441 gattttcttg tgaaggacac cgaagacgaa ataataccgc tgaaaaatat ttaacatgaa
     1501 atcattaaat gataacgata actgaactgg aagacgaaat aataaaaaat aaagaagccg
     1561 caaatatttt cattgaaaaa ataaacgacg aaaagaacga aatccatgaa aaaatgaaac
     1621 accccttgga taaagttacc tacaatgaag caaaggaact gctcattgcc tgcgatgcgg
     1681 caatcaggat catagagata atgctaatca gaattaataa taaatagagg acgagtattg
     1741 tgagccaaac aaaaaataga gaactgttag ataaaaaaat aagatcagaa attgaggtga
     1801 taaaaaaaat aatcgccgaa tttgatgtcg taaaagaaaa cgttaatgct ttaagtgaaa
     1861 aagcaaaaac agatcctcag gctgcagaaa aattaaacaa attaattgag gggtatacat
     1921 atggtgaaga gcgaaaatta tatgattctg ccttaagtaa gattgaaaaa ctaatagaga
     1981 caatgagtcc tccaagatct aaaaacca
//
LOCUS       DAZKHC020000278         2001 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZKHC020000278 DAZKHC020000000
VERSION     DAZKHC020000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.280"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1960
                     /locus_tag="ACHOSM_RS25185"
                     /old_locus_tag="pgaptmp_005037"
                     /pseudo
     CDS             1..1960
                     /locus_tag="ACHOSM_RS25185"
                     /old_locus_tag="pgaptmp_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
BASE COUNT          704 a          388 c          365 g          544 t
ORIGIN      
        1 aaaaccagga cccagaaatt tacctatcga caaccccaca ttgttatcat ggaacattac
       61 tgacggggat ctaaattcca aattaaatac attagaacat ctaaactgta taacaaatat
      121 tattaattct tgtggagttt accctcaagg attaaaagac agagaaatta tatcaacttt
      181 tcacgcagaa aaagttatta atgatctgtt aaaaaacgat tataaaattt ccctttctcc
      241 agatacaact tatcgagagt tgaataaagc agcacagcgt agcattacag cgccagacag
      301 gataggagaa ggaaaaacat gggtttatca acgagataca atgattgaaa gaggtgataa
      361 cagcggtgtt catcagtatg gtccagctga acatttcact cacattatat ctgacaaacc
      421 gtccccaaaa gataagtatg ttgcatatgc tattaacatt cctgactatg agctggcagc
      481 cgatgtatat aatattaacg ttacgtcaca ttccggacag caagaaacat ttaaaatact
      541 aatcaatcca gaacatctac ggcagacact tgagcgtaaa tctcttactg ctgttcagaa
      601 atcacaatgt gaaatcatca cccctaaaaa acctggcgaa gcgattcttc atgcttttaa
      661 tgccacctac cagcaaatca gggaaaatat gtctgagttt gcacgttgcc attatgggta
      721 tatacaaatc cctccagtga caactttccg caccgacgga ccagaaactc ccgaagaaga
      781 aaagggttac tggtttcatg cttatcaacc cgaagatctt tgtaccatcc ataatccaat
      841 gggagatttg caggatttta ttgcattggt taaagatgct aaaaaatttg gtatcgatat
      901 cattcctgat tataccttta actttatggg aattgggggt agtggtaaaa atgacctgga
      961 ttatccctct gctgatatac gagcgaagat cagtaaagat atagaaggtg gtatccctgg
     1021 ctattggcaa ggtcaggttt tgattccatt cattaaagat ccagtaacaa aagaacgtaa
     1081 acaaatccat ccagaagata tacatctcac tgcaaaagac ttcgaagcaa cacccaaaag
     1141 tgaccaggtt attatgttgc aaaatcaata cgttcgtgaa atgcgaaaat atggcgtacg
     1201 aggtttacgt tatgatgcgg caaaacactc aaaacatgaa caaatagaaa gatcaataac
     1261 cccaccgctt aaaaattata atgagcggtt acacaatact aacttattta acccaaaata
     1321 tcataaaaaa gccgttatga attacatgga atatctggta acttgtcagt tggatgaaca
     1381 acaaatgtca tcactgcttt atgaaagaga tgatttaagc gccattgatt tttcattgct
     1441 catgaaaacg ataaaagcct tttcatttgg tggagatctc caaacccttg catcaaaacc
     1501 gggttccaca atctcaagta tcccatcaga aagacggata ttgattaaca ttaaccacga
     1561 ttttcctaac aatggtaatc ttttcaatga ctttctattt aaccatcaac aagatgaaca
     1621 attagcaatg gcatatatgg ccgctctgcc gttcagcagg cctttagttt actgggatgg
     1681 ccaagtatta aaatcaacga ctgaaattaa aaattatgat gggtccacgc gtgttggcgg
     1741 taaggcgtgg cttaataaag gttgctctac ctatcagcag ctctacaatg aattccacgc
     1801 attatatata gataaagcag gaatatggag cgcatttgag ggtatatttg caactaaaaa
     1861 cgttctggcc tttagtcgtg gggattctgt gaacattaat cactctcctc atgatggact
     1921 agttataata aataaaggaa tcgaagaagt tgaaggtacc tagcctaaca aattgcaacc
     1981 tggaatatat aaaaacatgg g
//
LOCUS       DAZKHC020000279         1980 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZKHC020000279 DAZKHC020000000
VERSION     DAZKHC020000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.281"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..531
                     /locus_tag="ACHOSM_RS25190"
                     /old_locus_tag="pgaptmp_005038"
     CDS             <1..531
                     /locus_tag="ACHOSM_RS25190"
                     /old_locus_tag="pgaptmp_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4561326.1"
                     /translation="VSHIPAPRDGRDYDPEVLKQAVLEAVNALPAPQDGRDATALEVL
                     PAIDDQKSFPRGTYATHLGGLWRAYEKTHGMRGWECLVDGVADIDVSMTDERLFSVVI
                     RQSSGQCTEKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTGDKPGEAHSS
                     GWTLAAKRGRDAGGGK"
     gene            528..854
                     /locus_tag="ACHOSM_RS25195"
                     /old_locus_tag="pgaptmp_005039"
     CDS             528..854
                     /locus_tag="ACHOSM_RS25195"
                     /old_locus_tag="pgaptmp_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU4561327.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            865..1215
                     /locus_tag="ACHOSM_RS25200"
                     /old_locus_tag="pgaptmp_005040"
     CDS             865..1215
                     /locus_tag="ACHOSM_RS25200"
                     /old_locus_tag="pgaptmp_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU4561328.1"
                     /translation="MAISAGRLTQMISVLKPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            1212..1658
                     /locus_tag="ACHOSM_RS25205"
                     /old_locus_tag="pgaptmp_005041"
     CDS             1212..1658
                     /locus_tag="ACHOSM_RS25205"
                     /old_locus_tag="pgaptmp_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HGU4561329.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRCSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            1655..>1980
                     /locus_tag="ACHOSM_RS25210"
                     /old_locus_tag="pgaptmp_005042"
     CDS             1655..>1980
                     /locus_tag="ACHOSM_RS25210"
                     /old_locus_tag="pgaptmp_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HGU4561330.1"
                     /translation="MMEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATL"
BASE COUNT          406 a          477 c          688 g          409 t
ORIGIN      
        1 gtaagccata tccctgctcc gcgtgatggt cgtgattacg atccggaagt actgaagcag
       61 gcggttctgg aggcggtgaa tgcccttccg gctccgcagg acgggcgtga tgccacggca
      121 ctggaagtgc tccccgctat tgacgatcaa aaatcctttc cccggggcac gtatgccaca
      181 cacctgggtg gactctggcg ggcgtatgaa aaaacgcacg ggatgcgggg atgggaatgc
      241 ctggttgacg gggtggcgga tatcgacgtc agcatgacgg atgagcggtt gttctctgtg
      301 gtgatccggc agagcagtgg ccagtgtacg gaaaaaacat tttccctgcc ggtgatgctc
      361 taccgcggtg tgttcagagc cggtgaaacc taccaccccg gcgatacggt gacgtggggg
      421 ggctcgctgt ggcactgcaa cagtatgacc ggtgataaac ccggagaagc tcattcatca
      481 ggctggaccc tggctgcaaa acgtggacgg gatgcaggag gcggaaaatg acggcattac
      541 tgacactgga agagatcaag gcacatctgc gtgttgacca tgacgcggat gatgacatgc
      601 tgatggacaa ggttcgtcag gctaccgccg tgctgctggc ctacattcag ggtagccggg
      661 ataaagtgat tcgtgaggac ggtgagctga tcccgggcga ggcattaacc cggatgaagg
      721 gggctgccat gcgactgacc gggatgctgt accggaatcc ggatcttgct gagcgggaag
      781 acctcgtgca gggggaactg ccgttttctg tgtccgtgct gatttacgat ttgcgttgtc
      841 cgacggtgtt atgaggaggg aggaatggca atatctgcag gtcgtctgac acagatgata
      901 agtgttctga agccggtgtt aacccgtaat gctgccggag aaatgacgga agaatgggtg
      961 tcatgcggga aaattcatgc ggatatccgt ggcaggagca gccgggagcg gatgcagtcc
     1021 ggtgcggaaa tggcgcaggc ggaaatccgc atctgggtgc gcggtcagtc cggccgggaa
     1081 atcacggcag cgtcacgact tcatgtgctg agtggtccat ggcgtgaccg gatcctgaac
     1141 gttgtcgggc tgcccgtgcc ggatgcgacc ggcgggcgtc tggaaattct ctgtcggctg
     1201 ggaggggaaa aatgatcgaa accctgctgg atttttcggg gctggaggac atcagccgcg
     1261 atttgcagct tctgagtggt gcggaaaata accgggtgct gcgtgaggca acccgtgcgg
     1321 gtgcgaatgt gctgaaagaa gaagtggtgt cacgggcacc ggtacgcagg ggaaaacttc
     1381 gccgcaatgt ggtggtcctt tcccggtgct cccgcgatgg cgggatggaa tccggtgtgc
     1441 atatccgggg tgttaatccg gacaccggta acagcgataa caccatgaag gcggataacc
     1501 cgcgcaatgc tttctactgg cggtttgtgg aaatggggac cgtgaatatg ccaccgcacc
     1561 cgtttgtgcg cccggcattt gatgtgcgca gtgaacaggc ggcacaggtg gcgattgcgc
     1621 ggatgaaccg ggccattgat gaggtactga gacgatgatg gaggcggatt tgtatcctca
     1681 tctggcgcat cttgccggcg ggcaggtgta cccgtatgtg gtccccctgc tggatggcag
     1741 gccgtcggtg gcgcttccgt gggtggtttt cagcctgatt tcatcggtgt cggcggacgt
     1801 gatgggcggg caggcggagt cctcagtgtc ggtgcagata gacgtttatg ccgggactgt
     1861 gacgcaggcg cgtcagatac gtcaggacgc ccgtgaagcc ataatgctgc tggccccggg
     1921 atccgtcagt gaaatgcagg actatattcc ggaaaaccgc tgttaccgtg caaccctgga
//
LOCUS       DAZKHC020000280         1974 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZKHC020000280 DAZKHC020000000
VERSION     DAZKHC020000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.282"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            195..449
                     /gene="bssS"
                     /locus_tag="ACHOSM_RS25215"
                     /old_locus_tag="pgaptmp_005043"
     CDS             195..449
                     /gene="bssS"
                     /locus_tag="ACHOSM_RS25215"
                     /old_locus_tag="pgaptmp_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HGU4561331.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            564..1682
                     /gene="solA"
                     /locus_tag="ACHOSM_RS25220"
                     /old_locus_tag="pgaptmp_005044"
     CDS             564..1682
                     /gene="solA"
                     /locus_tag="ACHOSM_RS25220"
                     /old_locus_tag="pgaptmp_005044"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HGU4561332.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1730..1843
                     /locus_tag="ACHOSM_RS25225"
                     /old_locus_tag="pgaptmp_005045"
     CDS             1730..1843
                     /locus_tag="ACHOSM_RS25225"
                     /old_locus_tag="pgaptmp_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HGU4561333.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          523 a          472 c          456 g          523 t
ORIGIN      
        1 gcacaatatg ctaaaaaaat atgaatgaca tgctgtttta ttgtccatat aaatcttaaa
       61 agcagaaatt tatctgttcc cccctttaaa aattcggtga atacccttac ttattggtat
      121 actgaagttg cttcagataa aaacgcattg aacctcgaat aacgttgtct agtaacacga
      181 attagggggc catgatggaa aaaaataatg aagtcattca gactcatccg ctcgtagggt
      241 gggacatcag caccgttgat agctatgatg cgctgatgtt gcgtttgcac taccagaccc
      301 caaataagtc cgagcaggaa gggactgaag ttggtcaaac gctctggtta accactgatg
      361 ttgccagaca atttatttcg atattagaag caggaatcgc caaaattgaa tccggtgatt
      421 tcccggtaaa cgagtatcgg cgtcattaac atgacatgac catccaccca aaaggcacct
      481 caccggtgcc tttaccattt ctgggttgta tatctgcatt cagttaatta ctctgcttaa
      541 accatgagag cagagagaaa aaaatgaaat acgatctcat cattattggc agcggttccg
      601 taggcgctgc cgccgggtat tatgcaaccc gcgccggttt aaacgtgcta atgaccgacg
      661 cccatatgcc accgcatcaa cacggcagcc accacggcga tacgcgatta attcgccatg
      721 cttatggtga aggcgaaaag tatgtcccgt tggtcctccg cgcgcaaacg ctgtgggatg
      781 aactttcccg ccacaacgaa gatgatccca tttttgtacg ctctggtgtc attaacctcg
      841 gcccggctga ctccgcattt ctcgccaacg tcgcccacag cgccgaacag tggcaactca
      901 acgttgaaaa gctcgatgcg caagggatta tggcccgctg gccagaaata cgcgtcccgg
      961 acaactacat cggcttattt gagactgatt ccggtttttt gcgcagcgaa ctggcgatta
     1021 aaacctggat ccaactggcg aaggaagcgg gctgtgcgca actgttcaac tgcccggtca
     1081 ccgcaattcg tcatgacgat gatggcgtaa ctattgaaac cgttgacggt gagtatcagg
     1141 cgaaaaaagc gattgtctgc gcgggaacat gggtaaaaga cctgctcccg gagctgcctg
     1201 tccagcccgt acgtaaagta tttgcctggt atcaggccga tggccgctat agcgtgaaga
     1261 ataaattccc ggcgtttacc ggtgaactgc ccaatggcga tcaatattat ggttttccgg
     1321 cagaaaacga cgcgttgaag attggcaaac ataacggagg ccaggttatc cattcagcgg
     1381 atgaacgtgt tccgtttgcg gaagtggtca gcgatggttc ggaagccttc ccgttcttgc
     1441 gcaatgtatt gccgggtatc ggttgctgcc tgtacggcgc tgcctgcacc tatgataatt
     1501 cgcctgacga agattttatt atcgataccc tacccggcca cgataataca ctgctcatta
     1561 ccggcctgag tgggcacggt tttaaatttg cgtcagtttt aggggaaata gctgccgatt
     1621 ttgcgcaaga caaaaaaagc gattttgatt tgacgccatt caggctttcc cgcttccaat
     1681 aatcatcatt acggcctcca gaatgaggcc gtttttacag gagtatttaa tgcgtcggct
     1741 gttgcactat ctcatcaata atattcgcga gcatctgatg ctttatcttt ttctctgggg
     1801 attgttggca attatggatc tgatctatgt gttttatttt tagttttccg cactgacata
     1861 aactgacaaa tatttaatct ccctttcatt tagcgtgaaa aattgaatga gattattcct
     1921 gcttctacag cactcaccag acatctctga tgctgaaccc actaaactta tgaa
//
LOCUS       DAZKHC020000281         1965 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZKHC020000281 DAZKHC020000000
VERSION     DAZKHC020000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.283"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..155)
                     /locus_tag="ACHOSM_RS25230"
                     /old_locus_tag="pgaptmp_005046"
     CDS             complement(<1..155)
                     /locus_tag="ACHOSM_RS25230"
                     /old_locus_tag="pgaptmp_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhR family protein"
                     /protein_id="HGU4561334.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGG"
     gene            complement(263..339)
                     /locus_tag="ACHOSM_RS25235"
                     /old_locus_tag="pgaptmp_005047"
     tRNA            complement(263..339)
                     /locus_tag="ACHOSM_RS25235"
                     /old_locus_tag="pgaptmp_005047"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(303..305),aa:Val,seq:gac)
     gene            complement(344..420)
                     /locus_tag="ACHOSM_RS25240"
                     /old_locus_tag="pgaptmp_005048"
     tRNA            complement(344..420)
                     /locus_tag="ACHOSM_RS25240"
                     /old_locus_tag="pgaptmp_005048"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(384..386),aa:Val,seq:gac)
     gene            728..>1965
                     /gene="ydhQ"
                     /locus_tag="ACHOSM_RS25245"
                     /old_locus_tag="pgaptmp_005049"
     CDS             728..>1965
                     /gene="ydhQ"
                     /locus_tag="ACHOSM_RS25245"
                     /old_locus_tag="pgaptmp_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGU4561335.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFCVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSEVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGA"
BASE COUNT          534 a          413 c          552 g          466 t
ORIGIN      
        1 ccaccggctt cgtggttctt ttcactttct gtccatacct tccacagaaa accaggttcc
       61 tgattaatcg actcagcaag tggtttaagc tgctcagcca ttgcgtcgcc aaatgggcca
      121 ttaaaagcaa aatgaagttg taacaaagtt gccatagcga ttctccttct gataacgcat
      181 acgaagatag tgtaaatctt ttaacgtatt ggatacagag aagaaacaga gggaaaaagg
      241 aaaagaccag attaagaaaa tctggtgcgt ccgagtggac tcgaaccacc gacccccacc
      301 atgtcaaggt ggtgctctaa ccaactgagc tacggacgca ggatggtgcg ttcaattgga
      361 ctcgaaccaa cgacccccac catgtcaagg tggtgctcta accaactgag ctatgaacgc
      421 aacgttgtag gtgacaacgg ggacgaatat tagcggcaga gtgggaaggt ggcaagaggc
      481 aaaacgtaat tttctgcgct atttcgaccg tttgcagagc ttttaagcaa attggctata
      541 ttttgttgat ttgcaagggc gatttttatt caggatcgca tttacatctg atacaaccca
      601 ataattcgtg tatcagagtt taaacagagt tgtttgccac catctcaaat ctgtcgtgga
      661 taaatcctcc gtagccttta cacttaggta gcaataaagg cacacagatg cagcaaagga
      721 ataaatcatg ggatctgatg cgaaaaactt gatgagcgac gggaatgtgc aaattgttaa
      781 gaccggcgag gtcattggcg cgacgcaact tactgaaggc gagttgattg ttgaagctgg
      841 cggaagagcc gaaaataccg tggtcacggg ggctggctgg ttgaaagtgg caaccggtgg
      901 gatcgccaaa tgcacacagt acggtaacaa tggcacgcta tcggtcagcg acggtgccat
      961 tgccacagat attgttcagt ccgagggagg cgcaattagt ctctctacgc tcgctacggt
     1021 taatggccgc catcccgaag gtgaattttg cgttgatcag ggttatgcct gcggtttgtt
     1081 gctggaaaat ggcggtaacc tgcgtgtact ggaaggacat cgcgcggaaa aaatcattct
     1141 cgatcaagag ggcggcctgt tggttaatgg gacaacctca gaggtcgtgg tagatgaagg
     1201 tggtgaattg ttggtgtatc caggtgggga agccagcaat tgtgagatta atcagggcgg
     1261 cgtttttatg ctggccggga aagccagtga tacgttgctt gctggtggca ccatgaataa
     1321 tctcggtggt gaagactctg acactattgt tgagaatgga tccatttatc gtctggggac
     1381 ggatggcctt cagctctaca gttccggtaa gacgcaaaac ctgtccgtga atgtgggtgg
     1441 tcgggctgaa gtgcatgccg gtacgctgga aaatgcggta atacaaggtg gaacagtgat
     1501 cctgttgtca cccaccagcg cggacgaaaa ttttgtcgta gaggaagatc gcgcaccggt
     1561 tgaactgacc gggagtgttg cattactgga cggcgcttca atgattattg gctatggcgc
     1621 agatctgcaa caatcaacga ttactgtaca gcagggcggt gtgttgattc tcgacggcag
     1681 tacggtaaaa ggtgacagtg tcacttttag tgttggtaac atcaatctga acggcggaaa
     1741 actgtggctg atcactggtg cggcaacgca tgtgcaactg aaagtgaaac gcctgcgcgg
     1801 agagggagcg atttgcctgc aaaccagtgc gaaagaaatc tcacctgact tcatcaatgt
     1861 gaaaggggaa gttaccgggg atatacacgt tgagataaca gatgccagtc ggcaaactct
     1921 gtgcaacgct ctgaaattac agccagacga agacgggatt ggcgc
//
LOCUS       DAZKHC020000282         1960 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZKHC020000282 DAZKHC020000000
VERSION     DAZKHC020000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.284"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1250..1852
                     /gene="fimB"
                     /locus_tag="ACHOSM_RS25250"
                     /old_locus_tag="pgaptmp_005050"
     CDS             1250..1852
                     /gene="fimB"
                     /locus_tag="ACHOSM_RS25250"
                     /old_locus_tag="pgaptmp_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HGU4561336.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          588 a          351 c          406 g          615 t
ORIGIN      
        1 tataaaatct ctgctgatgt ttaattgatt tgaattttcg taagctatat cgcttactca
       61 atccatttta cccagagtca taaaatagag tgtctgttga tctcattttc atattccttt
      121 cagactgaag tgcgttttca tcacccgatg agaaagaact gaagtgatgt ttgccataga
      181 ttacgagatg cccagtctca ttgactatcg tggtgcacct ttatacctgt tataccagat
      241 caaaaatcac gcaatccata caacaaaacc agatttgcaa ttcgtgtcac aaaatatgtc
      301 gatcttttcc taagaggcag atgccatgtg aagccagacg aacacttgca ttggtcttca
      361 aaaactaaag gttttctttt aacaatctgt tttgtaaata atttatttag aatctaaaca
      421 aagaaagctt tgcaagctaa ctcagtgagc ttggtgaaaa taagtgttta cccgccatca
      481 ggctgagcat aattctcatc atgaaatatg tttcttggtt tgtggcttgt aactggtcac
      541 tcctgatgtc gatctggaga ggcttgttga tgttggtgtt ttcaggatga tgtttcactt
      601 agtttgtttg ccgtatcgcc cggcgaatag ctgtgattga ggaaggttta agtcgtagtg
      661 accaaagcca tagttaccaa cggatgtaga tgaaaaagcg tcgcctgcgt tcccccatat
      721 ctccaggata aaagggaatg taacaatctc atggcctaag ctgacgaacc aggcaggaat
      781 aatcgctagg gatctaagaa ttagcatgat aatagccact aagaaattac tgcgttccat
      841 gaaatagcca ttttgtggca aatgcagttg actaataatg tcatatgtga gacggctagt
      901 tgaacgaata ttaaattttg ctgaattttt tatgttgatt ttacttgtta cagaacatat
      961 cacatgatat atagataaga ttagttgcat taatgatgag ggttattatt cgattcgtat
     1021 cagatttata aatatataaa ggtacatagc atgcaagagc atggcgtttg tatggcaaag
     1081 ttattataag taactatcgc tactccattt aagttcactc agaagaactg gtccacttac
     1141 gttaattatt aagcaaacgt tcgcttttat aaacataatc aggattaaaa tgttggatta
     1201 ttgctaaccc agcacagcta gtgcgcgtct gtaattataa gggaaaacga tgaagaataa
     1261 ggctgataac aaaaaaagga acttcctgac ccatagtgaa atcgaatcac tccttaaagc
     1321 agcaaatacc gggcctcatg cagcacgtaa ttattgtctg actttgcttt gttttattca
     1381 tggtttccgg gcgagtgaaa tttgtcgatt gaggatttcg gatattgatc ttaaggcaaa
     1441 gtgtatctat atccatcgat taaaaaaagg cttttcaaca acgcacccgc tattgaacaa
     1501 agaagttcag gctttaaaaa actggttgag tatccgtact tcgtacccgc atgctgagag
     1561 cgaatgggta tttttatcgc gtaaggggaa tccgctttct cggcaacagt tttaccatat
     1621 tatctcgact tccggtggta atgccgggtt gtcactggag attcatccgc acatgttacg
     1681 ccattcgtgt ggttttgctt tggcgaatat gggaatagat acgcgactta tccaggatta
     1741 tcttgggcat cgcaatattc gtcatactgt ctggtatacc gccagcaatg cagggcgttt
     1801 ttacggcatc tgggataggg ccagaggacg acagcgtcac gctgttttat agagatagtt
     1861 gagataccag ggatggtgtt aaaaactaca ttattcttac gatatatcaa aaatgattaa
     1921 ccatttatta attgatgtta ttgtaagtgt gattcttaat
//
LOCUS       DAZKHC020000283         1959 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZKHC020000283 DAZKHC020000000
VERSION     DAZKHC020000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.285"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..641
                     /locus_tag="ACHOSM_RS25255"
                     /old_locus_tag="pgaptmp_005051"
     CDS             <1..641
                     /locus_tag="ACHOSM_RS25255"
                     /old_locus_tag="pgaptmp_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012600003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561337.1"
                     /translation="PVHWEVIMDKNVEHVLVDAIENKQSLTVVYLGGSQPGTLRNISP
                     ISINGDKLRARCHSSGAVKVFNLGKIQLPSDSCAVSMHYGDLEVKAYETMQSVNDNFH
                     ALYPEGRWGVDFNEHRFALFDFFKNGKRKKTAFMAIEFRERDEEKIITGVTIDIGISG
                     TVISEKSRIPKRRPWVVVGPEHGEYSTYSTLDKAATAFFERLSLIASGLEDN"
     gene            920..1117
                     /locus_tag="ACHOSM_RS25260"
                     /old_locus_tag="pgaptmp_005052"
     CDS             920..1117
                     /locus_tag="ACHOSM_RS25260"
                     /old_locus_tag="pgaptmp_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4561338.1"
                     /translation="MLKQQDMTETARVVFDELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            1252..>1959
                     /locus_tag="ACHOSM_RS25265"
                     /old_locus_tag="pgaptmp_005053"
     CDS             1252..>1959
                     /locus_tag="ACHOSM_RS25265"
                     /old_locus_tag="pgaptmp_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4561339.1"
                     /translation="MAVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
                     ATDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHM
                     SESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRHGYGQAIAEKCGYSSRSYFISVF
                     HRYYGFPPDRYVSRQGLD"
BASE COUNT          546 a          346 c          484 g          583 t
ORIGIN      
        1 tacctgttca ttgggaggtt attatggata aaaatgtaga gcatgtatta gttgatgcaa
       61 ttgaaaataa gcaatcttta acagtcgttt acttaggagg gagccagccc ggaacattaa
      121 ggaatatttc tccgattagt ataaatgggg ataaattgcg ggcaagatgc catagttctg
      181 gagcagtaaa ggttttcaat cttgggaaaa tacagttacc cagtgactcc tgcgcggtat
      241 ctatgcacta tggagattta gaagttaaag cttatgagac gatgcagagc gtaaatgaca
      301 actttcatgc cctttatcct gaaggacgat ggggtgttga ttttaatgag catcgctttg
      361 ctttatttga tttttttaaa aacgggaaac gaaaaaaaac ggcatttatg gcaattgagt
      421 tcagggaaag agatgaagag aaaataataa caggtgtaac aattgatatt ggtatatctg
      481 gaacagtgat ttctgagaag tcccgaatcc caaaaagacg accatgggta gtggttggtc
      541 ccgaacacgg agaatacagt acttattcaa ctttggacaa ggctgctaca gcgttttttg
      601 agaggctttc gttgatagca tccggcctgg aagataattg attttatgtt tggtattcag
      661 agttctccgt gcttaagaaa gtcaagattc taaaaatact gaatgagcta cttgtgttat
      721 aacaaaaatg ctattagtgt gttaagagtg gttacttcgc cacacaactt aaacccgccg
      781 ctgagcggtt tttttgtacc tgtaaacctt gtgcagtaca gtaaacacgc tggtggtcgt
      841 gaatactggc tttttatctt gctggctttt taggcaagag ttattggtat gtcacgttaa
      901 ccggaaaggg taaaaagaca tgctgaaaca gcaggatatg acagaaaccg ccagagttgt
      961 gtttgatgaa ttaagcgtta ccgaaccggc gacagtcggg gagattgcgc agaatactta
     1021 cctttcacgc gaacgctgcc agttaatact gacccagctt gttatggcgg gtctggcaga
     1081 ctatcagtgc ggttgttaca gacgccttca gtcctgaagg ctttttattt gtggtgaatg
     1141 ggcggctggt gggggggcga cacctgtcag tcctttgctt atgtgttgat gataatttac
     1201 cttttggggc tataattgag ctaaccaatt gctaatgaaa gtaaaattat aatggctgtt
     1261 gtctgttcag ttatcatggt ttgctcccca attaatattt ttcttgaaaa ggatacgttg
     1321 tcacttaagc caggctcagt tgttctggcc accaaatgca tcagggagct tttccttatg
     1381 cattatggca aagttaaaat tgtcgatata agcgaatccg tcgtaagtca atatctggaa
     1441 agtcagcata agctgacgag gactcgtctg actgacattc cgctttacct gttgctggaa
     1501 cccaacaatc ctgcgttggc tgcggcttta attaccagcc agggattttc cggagaggcc
     1561 acggatatgt ttcttatgat ggcctgcctg tctctgtttg aaacagatga acggatgtca
     1621 ttgtttttaa gtggatgttt atccagcata agtgccaaag tcagggcgat aattcagaca
     1681 gatatatcag caagctggac gcttggtgcg attgctctac agttgcatat gagtgagagt
     1741 ttgttaaaga caaaactgaa aaatgaaggg ggcatgttca gtcgcttgtt gctggaagag
     1801 cggatgcgtg ttgctgtaaa tatgttatgt tcccggcatg gatatggaca ggctatagca
     1861 gaaaaatgcg gttattcaag caggtcctac tttatttctg tatttcaccg ctattatggc
     1921 ttcccgccag acagatatgt atccaggcaa gggcttgat
//
LOCUS       DAZKHC020000284         1949 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZKHC020000284 DAZKHC020000000
VERSION     DAZKHC020000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.286"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..778)
                     /gene="nleH1-2"
                     /locus_tag="ACHOSM_RS25270"
                     /old_locus_tag="pgaptmp_005054"
     CDS             complement(<1..778)
                     /gene="nleH1-2"
                     /locus_tag="ACHOSM_RS25270"
                     /old_locus_tag="pgaptmp_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HGU4561340.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPNGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPVDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYED
                     MEDTTKVLKMFTISQSHEEVTSEVRCFNQYYGSGSAEKIYNDNGNVIGIRMNKINGES
                     LLDIPSLPAQAEQAIYDMFDRLEKKGILFVDTTETNVLYDR"
     gene            complement(885..956)
                     /locus_tag="ACHOSM_RS25275"
                     /old_locus_tag="pgaptmp_005055"
                     /pseudo
     CDS             complement(885..956)
                     /locus_tag="ACHOSM_RS25275"
                     /old_locus_tag="pgaptmp_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            950..1944
                     /locus_tag="ACHOSM_RS25280"
                     /old_locus_tag="pgaptmp_005056"
                     /pseudo
     CDS             950..1944
                     /locus_tag="ACHOSM_RS25280"
                     /old_locus_tag="pgaptmp_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          588 a          462 c          402 g          497 t
ORIGIN      
        1 tacgatcata taaaacattt gtttctgttg tatcaacaaa aagaattcct tttttctcca
       61 gtctgtcaaa catatcgtaa atagcctgtt cagcttgtgc tggtaatgat ggaatatcca
      121 aaagagattc cccatttatt ttattcattc taataccaat aacatttcca ttatcattat
      181 atattttctc agcactcccg gaaccataat actgattgaa acaacgaact tcgcttgtca
      241 cctcttcatg gctttgagat atagtaaaca tcttcaacac ttttgttgta tcttccatat
      301 cttcatacac gacagcatta ccacctttac caataacatt acctggcacg ggtgatttat
      361 tgttgctaac cctcggcaac tctgactgag tataatccgc tggaggtaac tccggctgcg
      421 cataatccac tggaggtaaa tcaggtctgt tcgaatgaac agagcctctt tctatagtcg
      481 tgctcactgg cgatgtattc agcatagcct caatttttct gctaatctct ccgttaggcc
      541 atcccaacct gtgcaacaga ttagtaaaac aaccactatg actttctctt gtaacgcaaa
      601 acttattatc agtgaccaca acacgatatg ttctgttgcc aaccgttact tgcgtcccgc
      661 tatcagagtg aacagcagca tcccttacag aggataaaac acgattatca ggcgaagtca
      721 ggtttctggt taaagaattc catgaacatc ccaaatttat agaagagggc gataacatac
      781 atttcaacct tcaaaataaa cctatctaat tattcctaac agacatcccc catacatgac
      841 aacaaaaacc ggagccggac tccggttttt gtgaagctat cgggttactt catttcgcca
      901 atattttccc acttcccgtc agcacgcagg atttgcagcg gtcttactac acactgaccc
      961 gaggacgaaa aaagagttcg ggcagggaag agacagaaga gtagcaattt cagaagtatt
     1021 cacaccaaca ttgagctttt cagcgaaagt gggcacgaat cattgctgga cagggttaaa
     1081 ggcactgact caatcactct tcatacgtgg cttgatcgat atgaaacaat cctcagctag
     1141 agggggagca agccgaaaac attactcgac tacgccagca aaatcagggc aattcgaaga
     1201 aaattgccgg acaaaccgct cactgacata tcaacgaaag aggtggcagc aatgctaaac
     1261 acctacgtcg cagaaggtaa agcggtttcc gcaagagtaa tcaggtcaac ccttgttgac
     1321 gtttttcgag gggcaatagc cgaggggcat gtggcaacga atccagtaac aacaacccgt
     1381 gcagcaaagt cagaagtaag gcgctcaagg ctgacagcta atgagtatgt cgctatttac
     1441 catgctgctg agcacctccc catctggctg aggctgtcaa tggatttagc tgtcgttaca
     1501 gggcagagag tgggcgattt gtgcagaatg aagtggtcag acataaacga tggtcatctt
     1561 cacattggac agagtaaaac aggagccaaa attgccattc cgctggctct aaccattgac
     1621 gcactcgaca tctcactggt tgatacacta cagaaatgca gggaggccag cagcagtgaa
     1681 acaataatcg catcaaccta tcacgaacca ctttctccag ccacagtatc acggtattta
     1741 acaaaggcgc gaaatgcatc cgggatctcg tttgatggag acccaccgac atttcatgaa
     1801 ctacgtagtc tgtccgcgag gctatatcgg aaccagattg gctacaagtt tgcacaacgt
     1861 cttcttggac ataaatctga ttcaatggcg gcgcattata gggacagtcg cgggcgggag
     1921 tgggacaaaa ttgaaatcgg ataatgatt
//
LOCUS       DAZKHC020000285         1924 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZKHC020000285 DAZKHC020000000
VERSION     DAZKHC020000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.287"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            98..1423
                     /gene="nleA"
                     /locus_tag="ACHOSM_RS25285"
                     /old_locus_tag="pgaptmp_005057"
     CDS             98..1423
                     /gene="nleA"
                     /locus_tag="ACHOSM_RS25285"
                     /old_locus_tag="pgaptmp_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HGU4561341.1"
                     /translation="MNIQPTRQSGITSQNNQHHQTEQIPPTQIPQSELPLGCQAGFVV
                     NIPDDIQQHAPECGETTALLSLIKDKGLLSGLDEYIAPHLEEGSIGKKTLDMFGLFNV
                     TQMALEIPSSVSGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGFNFKDLPGPL
                     KNALSNSNISTTTVSTIASTGTSATTSTVTTKPKDPIPWFGLTAQVVRNHGVELPIVK
                     TENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDL
                     DNPNTKYAVLTNAAANALGALGGFAVSRFASTDPMLSPHIGAMVGQAAGHAIQYNTPG
                     LKPDTILWWAGATLGAADLNKAEFEVARFTDYPRIWWHAREGAIFPNKADIEHATGAD
                     IRAMEEGIPVGQRHPNPEDVVIDIESNGLPHHNPSNHVDIFDIIQETRV"
     gene            complement(1763..>1924)
                     /locus_tag="ACHOSM_RS25290"
                     /old_locus_tag="pgaptmp_005058"
     CDS             complement(1763..>1924)
                     /locus_tag="ACHOSM_RS25290"
                     /old_locus_tag="pgaptmp_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU4561342.1"
                     /translation="AVCCLFDFDAFSRLASEGSYHPLTREPITASMIISPDKCVYDPI
                     KGNFIIKDS"
BASE COUNT          647 a          387 c          355 g          535 t
ORIGIN      
        1 agttatatat taaagctgtc cacatcggat atgtgacact aatagtatca atggattgat
       61 attattaatg gatataaaca tgtaataagg atttattatg aacattcaac cgaccagaca
      121 atctggaatc acctcacaaa acaatcaaca tcatcaaaca gaacaaatac cccctacaca
      181 aataccgcaa tccgaattac ctctaggatg ccaagctgga tttgttgtta atattccaga
      241 tgatatacag caacatgcac cggaatgcgg tgaaacaaca gctctactga gcttgataaa
      301 agataaaggt ctgctctcag ggctagacga atatatagct cctcaccttg aagaaggatc
      361 cataggaaaa aaaacattgg atatgtttgg tttattcaat gttacccaaa tggcattaga
      421 gatacctagt tccgtttcag gcatctctgg taaatatggt gtccagctaa acattgtaaa
      481 accagatatt catcctacat caggtaatta ttttttacag atattccctc tgcatgatga
      541 aataggtttt aattttaaag accttcctgg cccgttaaaa aatgcattaa gcaacagtaa
      601 tatatcaacc actacagtgt cgactattgc atcgactgga acatcagcca ctacttcgac
      661 ggtaaccacc aagccaaaag acccaatacc atggtttgga ttaacagctc aagtggttcg
      721 taatcatggt gtagaacttc ctatagtcaa aactgaaaat ggatggaagc ttgttggaga
      781 aacaccactt actcctgatg ggccgaaagc aaattacacg gaggagtggg ttatcagacc
      841 gggagaagca gattttaaat atggtgcatc tccattacag gcaactctag ggctggagtt
      901 tggcgcacat ttcaagtggg atttagataa ccctaatact aaatatgccg ttcttaccaa
      961 tgctgccgca aatgcgcttg gtgctttagg gggatttgca gtatccagat ttgctagtac
     1021 agatccaatg ttaagtcctc atatcggtgc aatggttggg caagcagcag ggcatgccat
     1081 acagtataat acccctggat taaagccaga cactatttta tggtgggctg gtgcgacact
     1141 gggggctgcc gatttaaaca aggccgagtt tgaagtagct agattcactg actatcctcg
     1201 tatatggtgg cacgcaagag aaggagctat tttccccaat aaagcagata ttgaacatgc
     1261 cacaggtgct gatatacgcg caatggaaga aggtatccct gttggacagc ggcatccaaa
     1321 tccagaggat gtggtaatcg atatcgaaag caatggctta ccacatcata atccatcaaa
     1381 tcatgttgat atctttgata taatccaaga aacaagagtc taagctttaa cttgtttatt
     1441 ttaaaataat agctgaaatg gatggcagct attattaata aggattaata tatttttttc
     1501 taaaataccc cacaaaataa acacatcata aaaattctaa atacacacac ctaatctcaa
     1561 aaaaacaaac ttactatttg acttggatta tgtttttaat ccaatatttt ccgacaacat
     1621 ctccacagaa aaatgcattt acataaagac gccttcaagt catctgaaac ctgccaacca
     1681 cagacacctt cttatattct ggatgtctgc ggcgtttttt tacaaattac tgacagccaa
     1741 tactctctca gagaaaaata ttttaactat cttttataat gaagtttccc ttgataggat
     1801 cataaacaca tttatcagga cttataatca ttgatgccgt tattggttct cgggtcagtg
     1861 gatgatatga gccttcacta gctaaacgag aaaatgcatc aaaatcaaat aagcagcata
     1921 ctgc
//
LOCUS       DAZKHC020000286         1919 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZKHC020000286 DAZKHC020000000
VERSION     DAZKHC020000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.288"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..456
                     /locus_tag="ACHOSM_RS25295"
                     /old_locus_tag="pgaptmp_005059"
     CDS             <1..456
                     /locus_tag="ACHOSM_RS25295"
                     /old_locus_tag="pgaptmp_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGU4561343.1"
                     /translation="KSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIA
                     EQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNA
                     AELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLTSVLAGSGLS"
     gene            467..904
                     /locus_tag="ACHOSM_RS25300"
                     /old_locus_tag="pgaptmp_005060"
                     /pseudo
     CDS             467..904
                     /locus_tag="ACHOSM_RS25300"
                     /old_locus_tag="pgaptmp_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            904..1281
                     /locus_tag="ACHOSM_RS25305"
                     /old_locus_tag="pgaptmp_005061"
     CDS             904..1281
                     /locus_tag="ACHOSM_RS25305"
                     /old_locus_tag="pgaptmp_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561344.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            1750..>1919
                     /locus_tag="ACHOSM_RS25310"
                     /old_locus_tag="pgaptmp_005062"
     CDS             1750..>1919
                     /locus_tag="ACHOSM_RS25310"
                     /old_locus_tag="pgaptmp_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HGU4561345.1"
                     /translation="MELKKLMEHISIIPDYRQVWKVEHKLSDILLLTICVVISGAEGW
                     EDIEDFGETHLD"
BASE COUNT          635 a          284 c          463 g          537 t
ORIGIN      
        1 aagagtcacg gcagagaaga aatccgtctt catattgttt gcgatgtccc tgatgaactt
       61 attgatttca cgtttgaatg gaaaggactg aagaaattat gcgtggcagt ctcctttcgg
      121 tcaataatag cagaacaaaa gaaagagcca gaaatgacgg tcagatatta tatcagttct
      181 gctgatttaa ccgcagaaaa gttcgccaca gcaatccgaa accactggca cgtggagaat
      241 aagctgcact ggcgtctgga cgtggtaatg aatgaagacg actgcaaaat aagaagagga
      301 aacgccgcag aattattttc agggatacgg cacatcgcta ttaatatttt aacgaatgat
      361 aaggtattca aggcagggtt aagacgtaag atgcgaaaag cagccatgga tagaaactat
      421 ctcacgtcag tccttgcggg gagcgggctt tcgtaatctt tccctgggga gtcagccgaa
      481 tgttgggaag gatttgacgg atgaagagaa gaactgcctg gccggcggca gtacgggaag
      541 cccgggagga tggggaccag aagatgagaa tagcgcaagg gatcgtatgc agcatgaacg
      601 gtataaagat gaattgcgac agaaaatggg aaaacccgac gttacagatt caaatttgaa
      661 aaatattata gacgatcttt atcggccaaa tgcaaaagta ggaagtggaa gcacagcaga
      721 tgctgttcgt tatgaattgg ccacaggtga aaaagttggt ggtagaggac atgtattgaa
      781 agcacaggat tatacaaggg ctttacagga ttggttggat aagaatccaa cagcatctcc
      841 aggggatcga gcagcagcag aaaacgttct tcaggattta cagaatgctt tgggaggcaa
      901 gtaatgaacc aatatattgc tagttatata gaaaaaatgg catcattttc cactgttttg
      961 tctgagacag tctcattagc taatgaatac tggcatccag aaacgccacc ggtggttatg
     1021 ttgttctctc aggttggaaa aacgattgta aaaaattttt atgaaatggg ggatattgaa
     1081 agagaaaaaa tatttaaaca tgtcgaagat ggtatggtct cgcttgatga tgaattggcc
     1141 acagttgtag ctacagggct tatagaggca atagtcactg cgaccgatag caattcttta
     1201 ctttggcaag atttaagtaa atttctgggt gacaaatcaa agtctcatgc attagtgtgg
     1261 aaaaattttg gtcaggagtg atattattca agaagtatta agctcgctat tgagatctta
     1321 atgcttcttg agattaaatt aaagttagga cgaagtaatt gcaggtggaa ataaatccat
     1381 ggtgacgtgt ggcaatggct attagatgtt atggtgtgat gggctttggt gatttaagag
     1441 ctaagttaga ttgccttatg aaaaagtatg gttatatatt taatgatgtg tatcatatat
     1501 ttaaataaaa aacggttctc tcttgtcaag atagtaatag gatagattat tgtgttgata
     1561 aatataatta agagtctact ttgttgattt tagaagactt tggagcaggg aaagatcatc
     1621 agaagtacac tttttgtact aaataattcg cgctaaatgt tcaataattg agatattcct
     1681 tattgcctaa aactattttt catagcttat atatgatcaa atactccttg cataaataag
     1741 gagaacaaaa tggaacttaa aaaactgatg gaacatattt ctattatccc cgattacaga
     1801 caagtctgga aagtggaaca taaattgtca gatattctac tgttgactat ttgtgtcgtc
     1861 atttctggtg cagagggttg ggaagatata gaggattttg gtgaaacaca tctcgattt
//
LOCUS       DAZKHC020000287         1898 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZKHC020000287 DAZKHC020000000
VERSION     DAZKHC020000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.289"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1079)
                     /locus_tag="ACHOSM_RS25315"
                     /old_locus_tag="pgaptmp_005063"
     CDS             complement(<1..1079)
                     /locus_tag="ACHOSM_RS25315"
                     /old_locus_tag="pgaptmp_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU4561346.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLETDARKTAGVV
                     EQNLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGSLAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQASESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDAA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYVNTAAEAIRALSMQMPG"
     gene            complement(1060..1464)
                     /locus_tag="ACHOSM_RS25320"
                     /old_locus_tag="pgaptmp_005064"
     CDS             complement(1060..1464)
                     /locus_tag="ACHOSM_RS25320"
                     /old_locus_tag="pgaptmp_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4561347.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRTHYFLDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLAPRREEAQTEMPDEEKMLMQKAAGLAGGVRFGGDGGRE
                     ILSSADVSEDDVALMMASAGISGGVRYVPAGW"
     gene            complement(1491..>1898)
                     /locus_tag="ACHOSM_RS25325"
                     /old_locus_tag="pgaptmp_005065"
     CDS             complement(1491..>1898)
                     /locus_tag="ACHOSM_RS25325"
                     /old_locus_tag="pgaptmp_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4561348.1"
                     /translation="EYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGNLQVSVEDL
                     VRTGAFLVAMSLWHNHPQKTASPSMNEAVMQIEQEVLTTWPADAIARAEDVVLRLSGM
                     SGAVHADTDSTEVAKNNALTDDDFSAGKSSTAS"
BASE COUNT          415 a          614 c          471 g          398 t
ORIGIN      
        1 aagcccggca tctgcatcga cagggcacgg atggcttccg ctgccgtgtt cacataccac
       61 tgccattttt tatccgcctc atcaaatgcc ttctgtgcac tggcgagcat atccgctgag
      121 gactcaggac gaccgatatc cagaatggca tcccacatcg atttgaatgc cttccctgtt
      181 ttatccgccc aggtctccag tgtccccatg ttttctttca ggcgacgggt ctgctcatca
      241 aagcctttcg tggcagcatc gttcgccgcc tgtaaggccc cggcctcatc accggaacgc
      301 tgcagctgcg caacatacgc aatctgctct gccgtcacgt tacggaactg gcgcgccatc
      361 gccatcagcc ccgacgtcgg gtcggtggtc agttttccga aagcctctgc aaccttgtcc
      421 acctccacac cggatgcaga agcaaaacgc gcgacactct ggttgatggc atcaaactgt
      481 tcaccaccac gcacaccggc attcaccagg gctgccagtg actcactcgc ctggttaaac
      541 gtcagccctg cggcctgtcc ggctctggaa agcgtcagca tgcgatcggc agtcagtccg
      601 gactgattac cggaaagaac cagggtttta ttaaacgctg aaagcgtgga atccccctgg
      661 taccaggcgt acgccagcga acctgtcgcc accgccagcg aggtgacccc gaccatcggc
      721 agggtgatcg caccggcaag tcccctgaac atggggatca tcccgccgaa ggagtccttc
      781 acctgaccgc cctgttgcag caggatcagc cagggattct gaccaccggc aagctgcgtg
      841 gcgatatccg taaactgtgc gggcagggtt cgcatggccg ctttatactg cccgacggaa
      901 atcccggctt tttgtgcagc cagcgcctga cgactcaggt tctgctcaac gacaccggcg
      961 gtttttctgg cgtcagtctc cagtccggaa aaatgacgcc ttacccggct catctgctca
     1021 tcgaaacgga ccgcatccag actcaggtca ataacaagat caccaaccgg ctgggacata
     1081 tctcacacct ccggaaatcc ccgctgaagc catcattaat gcgacatcat cctcgctgac
     1141 atccgcagac gataaaatct cacgccctcc gtccccacca aaccgtacgc ctccggcaag
     1201 tcctgccgct ttctgcatca gcattttttc ctcatccggc atctccgtct gcgcttcctc
     1261 acgccggggg gcaagcagac tgaaatcaga gggatgcata tccggatcgc aaaaaaacag
     1321 gctgagtacg gcgtacgtca gcccggaaaa atgcatatcc agctgggtat cgagaaaata
     1381 atgcgtacgg taaaaatgtc gccagtcggc atattcggtg gatgtcatcc cggcaagcat
     1441 ggcgcgccag tcgggtctcc ccatctcacg cgccagtctg agggcaaaat tcagctcgcc
     1501 gtcgaagact ttcccgcaga aaaatcatca tcagtcagcg cgttattttt cgccacttcg
     1561 gtgctgtcag tatccgcatg aacagccccg ctcatcccgg acagacgcaa caccacatct
     1621 tccgccctgg caatggcatc cgcaggccag gtggtgagca cctcctgttc gatctgcatc
     1681 acagcctcat tcattgacgg tgatgccgtt ttctgcggat ggttatgcca cagggacatc
     1741 gccaccagaa acgcgccggt tctgacgaga tcttccacgc ttacctgcag gttgccgctg
     1801 gattctgcct gttctgcacg ccgtttcagg agggcaagat gctcaatacg ctgcagcgca
     1861 gacaattcgg aaagcgtgac agacactccg ttatattc
//
LOCUS       DAZKHC020000288         1882 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZKHC020000288 DAZKHC020000000
VERSION     DAZKHC020000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.290"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            301..1542
                     /locus_tag="ACHOSM_RS25330"
                     /old_locus_tag="pgaptmp_005066"
     CDS             301..1542
                     /locus_tag="ACHOSM_RS25330"
                     /old_locus_tag="pgaptmp_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU4561349.1"
                     /translation="MNLQSGQNIPLQQSTIRLNLQYPAKSGFKGEPDTCLFMLNAQGK
                     VSGDSDFIFYNNLSSPEGAVRLVTGSQQASIEIALDRVPANVSKIAITVVIDGEDTIS
                     GLSLLSIQAPGIADFQAETQGRSEKAIILGEVYRHNGAWKLRALGQGFNGGLEPLAIN
                     YGVDVAQPAPQPAKPARISLEKKLETRSPRLVSLAKKASVSLTKNKLDTLEAAVAFVL
                     DASGSMSGQFSKGNVQSVLDRIAVLAAQFDDDGEMDVWGFGEKHKKYPNVTLDNLDTY
                     IQSIRGAGKRSSWENLPGLGGTNNEPPVMEEIVDYFKDSKIPVYVVFITDGGISKTRA
                     IKDAIRRSANYPIFWKFVGLGGSSYGILKNLDDFTDRRVDNTHFFAMDDFGSISDEKL
                     YDNLLEEFRPWIDETKRLGIL"
     gene            1700..>1882
                     /locus_tag="ACHOSM_RS25335"
                     /old_locus_tag="pgaptmp_005067"
     CDS             1700..>1882
                     /locus_tag="ACHOSM_RS25335"
                     /old_locus_tag="pgaptmp_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4561350.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVS"
BASE COUNT          518 a          419 c          464 g          481 t
ORIGIN      
        1 ttttatgcag gccccgggtt tatctgtggt tgctggttaa agccagtatt ccgtgtctga
       61 tatcaacagc atgcccttcg gatatatgtt acttttgcga cttatgaagg aacgggccct
      121 ggaaaataag atacccggtc aatgaaaatt ccaattcaac aggtatagaa acttcagcgt
      181 tatgaaaaat ggtgtcatac catatttacg cctcaccgaa tatccagcgt ccagaactaa
      241 gcctagggaa catatataaa cgctgcgcat taataataat caaaaatcag gagagttaat
      301 atgaatttac aatccggaca aaacataccc cttcagcaat ctacgatcag gctgaatctt
      361 cagtaccctg ccaaatccgg ctttaaaggc gaacccgata cctgcctgtt catgcttaat
      421 gctcagggaa aggtcagtgg cgattctgac tttatctttt ataataattt gtcttctcct
      481 gagggggcag tcaggctcgt taccggttcg cagcaggcca gcattgagat agcactggat
      541 cgtgttccgg cgaacgtcag taaaattgcg atcaccgttg tcattgatgg tgaagatacc
      601 atcagtgggc tcagtttatt gagcattcag gctcctggta tcgctgattt ccaggctgag
      661 actcagggcc gcagtgagaa agcaatcatt ctgggtgaag tatatcggca caatggcgcc
      721 tggaagcttc gtgcgctagg gcagggtttc aacggtggac ttgagccttt ggccattaac
      781 tatggcgttg atgtcgcaca gccagctccg cagccagcaa agcctgctcg tatcagtctg
      841 gaaaagaaac tggaaacccg atctccgcgc cttgtaagcc tcgctaaaaa agcctcggtc
      901 agtctcacta aaaataaact ggacaccctt gaggctgcgg ttgcgttcgt acttgacgca
      961 tccggctcga tgagtggcca gttcagtaag ggtaacgttc agtctgtgct ggaccgcatc
     1021 gccgttcttg ccgcccagtt cgacgacgac ggtgaaatgg atgtctgggg atttggagag
     1081 aagcataaga agtatccaaa cgtcactctg gacaatctgg acacgtatat tcagtcgatt
     1141 cgaggggctg gtaagcgttc atcctgggag aacctgccgg gccttggggg gacaaacaac
     1201 gagcctcctg tgatggaaga aatagtcgac tactttaagg actcgaaaat cccggtatat
     1261 gttgtattta ttaccgatgg cgggatcagc aagacccggg cgattaaaga tgcaatccgg
     1321 cgttctgcca actaccccat cttctggaag tttgtcggcc tgggtggttc aagctacggt
     1381 atcctcaaaa atctggatga ctttactgac cgccgggttg ataacaccca cttctttgcc
     1441 atggatgatt tcggttcgat tagcgatgaa aagttatatg ataatttact ggaagaattc
     1501 agaccgtgga tcgatgaaac aaaaaggtta ggcatccttt aatacatata gttagagcat
     1561 gggtgaatga gctctacagc agcttaatac tgtagagcct ttcttcatta aaaggtaaac
     1621 gtaaaccgac cgccatatgt agccattaga caagaattgg taacttagac gcccatctga
     1681 cacagacgga catctaagta tggaattaca ggactggcga aaagaacctc gtaaaaagta
     1741 ttcgaatgaa ttcaaacttc gtatggttga actggcatca caacccggtg cttgtgttgc
     1801 acagattgca cgtgaaaatg gcgtcaatga taatgttatt ttcaaatggc tcaggctctg
     1861 gcagaacgaa gggcgtgttt cg
//
LOCUS       DAZKHC020000289         1877 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZKHC020000289 DAZKHC020000000
VERSION     DAZKHC020000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.291"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            197..844
                     /gene="nleG"
                     /locus_tag="ACHOSM_RS25340"
                     /old_locus_tag="pgaptmp_005068"
     CDS             197..844
                     /gene="nleG"
                     /locus_tag="ACHOSM_RS25340"
                     /old_locus_tag="pgaptmp_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HGU4561351.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPPPLSREPFVPEMIVSKDECHFNLIEQYFCILATQNICTRI"
     gene            complement(981..1127)
                     /locus_tag="ACHOSM_RS25345"
                     /old_locus_tag="pgaptmp_005069"
     CDS             complement(981..1127)
                     /locus_tag="ACHOSM_RS25345"
                     /old_locus_tag="pgaptmp_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561352.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGTDCNVKYKTL
                     KIPF"
     gene            1555..1833
                     /locus_tag="ACHOSM_RS25350"
                     /old_locus_tag="pgaptmp_005070"
     CDS             1555..1833
                     /locus_tag="ACHOSM_RS25350"
                     /old_locus_tag="pgaptmp_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HGU4561353.1"
                     /translation="MPFSIKSIFSGHTWHQPEISRPIADKSSTKNCILDSTTCNVDGF
                     TVFNRRSCSFDMRPPGSADRTPQLRLSISEVAWMSKIIETETNNTNKS"
BASE COUNT          613 a          371 c          331 g          562 t
ORIGIN      
        1 catttactgg cataacaaat ccttttcatt aaattttgct tacaaaaatt gtatacaatc
       61 attttaatta aaaaaataca tgtgtaaata gcattcgttc aaaatttaat tttttgtttg
      121 aatcctatca catatttagt atcttgactc ctgtcaccaa tatggttcaa taacatagac
      181 taaagaggga gaaaatatgc ctgtcatatt aaacttttcg agtgaaagag tattgtctga
      241 aagtgaactg gaggctttac gtcatgtcgg aagagttagt cagagtgaac agcttgttgt
      301 gagaggccgc actatgagac ttcatcatat ttcatttatg gacagtttta gcgtggaacc
      361 ggtttccggc ggactgttgg atcgccttag tgcaagaggg catcgccttc tcgcagaaaa
      421 tctggagata cagcttaaca gaggccatac ttttttgcag gcattccgtt tatatatgga
      481 acagagccgt gcaaccccat gtacgcgcca aaacgtcagt agtgcaatac aaaacaaaat
      541 taactctcat gcatttactg ttagtcatca agacttttcc tgtcatgaac aacacctgaa
      601 ttgcccgata acattatgca ttcctgaaac tggtgttttt gtcagaaatg caaaaaactc
      661 agaaatatgt tcattatatg accacaatgc actcactgaa cttatccgac gcaacgctcc
      721 cccccctctc agccgtgaac cctttgttcc ggaaatgatt gtcagcaaag atgaatgcca
      781 ttttaatcta atagaacaat atttttgcat attagccaca caaaatatat gcactagaat
      841 ttgactgtat ggttcgattt aaatgttgca aaattaattt tactagttat tgccatctat
      901 ttcatactcc ctaattcttg atacaatctg tctgtcaaaa tagtagatta gatcgaactt
      961 atattacagc tatttagttt ctaaaatgga atttttaacg ttttatactt cacattacaa
     1021 tctgtaccat aagcctcctc agcgaggttg cctgaatacc ctgtcgtgcc catttttcca
     1081 tcggacgcag ttgtctgctc tctcgaactg ctggcaataa aaatcaacca gacagcttga
     1141 tgtccgttac atgcaggact aatcacgatt ggttaccgtt tacaaaactt gcgtaacatt
     1201 agaggagaac gaataatatt ctctagctgg tacgcgttcc atagcgaaac gggcaaggtc
     1261 caacagcaag agctgaaact cagggggatt tatcagtcga tttattcgcc atggatgaat
     1321 cacgctgtac ggttatggta gctcttagcg acaaggcata atcatcagta caccaatgta
     1381 acaaccgttg aaaaagactc attattttat gaaccagcca tcccatttcc agagtggtaa
     1441 atatgatttg ttaaagtgta acagtactct gcgtagcctt tttagattca tagtttaatg
     1501 tttctaaata cattaaattg cactataaga gaaagacata atgtgaggat aaaaatgcca
     1561 ttttcaataa aaagtatttt ttcaggacac acttggcatc aacctgaaat atctaggcct
     1621 atagcagata aatcatctac taaaaattgt atcttagact caacaacatg caatgtagat
     1681 ggctttacag tgtttaacag aagatcctgt agttttgata tgcgtcctcc cggaagcgca
     1741 gacagaactc cacaactgag attatcaata tcagaagtcg catggatgtc aaaaataata
     1801 gagacagaga caaataatac aaacaaatca tagctattag cccagaaaag agttggtaca
     1861 ctagccatga ataactt
//
LOCUS       DAZKHC020000290         1851 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZKHC020000290 DAZKHC020000000
VERSION     DAZKHC020000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.292"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..220)
                     /locus_tag="ACHOSM_RS25355"
                     /old_locus_tag="pgaptmp_005071"
     CDS             complement(<1..220)
                     /locus_tag="ACHOSM_RS25355"
                     /old_locus_tag="pgaptmp_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4561354.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSQHGNVRISIERLAKRGVIQLP
                     SMQKVENKQTISPNKFTSVYIFEGEQGKR"
     gene            complement(372..1421)
                     /locus_tag="ACHOSM_RS25360"
                     /old_locus_tag="pgaptmp_005072"
     CDS             complement(372..1421)
                     /locus_tag="ACHOSM_RS25360"
                     /old_locus_tag="pgaptmp_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HGU4561355.1"
                     /translation="MCMIDLSITTRDMLMKNDDVSGKAKGGKARAAKMTAEQRKESSR
                     KAVAAKKEKALLPVSANEGKLKIGDAELDVAVLENGRRIISQASVFKAFGRPQRGGRA
                     PQEEGVINMPAFMDAANLKKYINQDVMDVINKVKYKTITGSVQEGYDASIIPLVCDVY
                     LKAREAGAITRPNQLETAKKAEILVRSLAKVGIIALVDEATGYQRDREKDALAKILEA
                     FVAKEIQPYITTFPADYYEELFRLRGLEYPPENPRFRPQYFGVLTNDIVYKRLAPNIL
                     EELKKQNVKASKGTKLFQGLTPNIGYQKLREHLSSTVTIMKLSNDYSDFIAKMNRLHP
                     RFEDVKTDELDDSDK"
     gene            complement(1529..1606)
                     /locus_tag="ACHOSM_RS25365"
                     /old_locus_tag="pgaptmp_005073"
                     /pseudo
     CDS             complement(1529..1606)
                     /locus_tag="ACHOSM_RS25365"
                     /old_locus_tag="pgaptmp_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
     gene            complement(1603..>1851)
                     /locus_tag="ACHOSM_RS25370"
                     /old_locus_tag="pgaptmp_005074"
     CDS             complement(1603..>1851)
                     /locus_tag="ACHOSM_RS25370"
                     /old_locus_tag="pgaptmp_005074"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU4561356.1"
                     /translation="MLVPEDMSVGWFSKALESVDEVRIITDGRINFIEPSTGLEKKGN
                     SKGSMLLIWRPFISPRRMFTTVSKAALMAIGQGVRRAA"
BASE COUNT          447 a          439 c          363 g          602 t
ORIGIN      
        1 ctcgcttacc ttgttcgcct tcgaatatat acacgcttgt gaatttgtta gggctaattg
       61 tttgtttatt ttcaactttt tgcattgaag gaagttgaat caccccacgc tttgccagac
      121 gttctattga tattctgaca ttaccgtgtt ggcttcccac caactcagcg atttcaatgc
      181 ttgtcatttt gatggcattg ctatttatca gctcattcat tgtcatgtcc tctcacattg
      241 aaaattcagc aataaaaaac ccagccgaag ctgggtttgt taagttgtca atggtcagta
      301 gtaatgcagt gaaggaggta aacttacgct gaaggatttg tacaaaaaaa ccacctgaag
      361 gtgggttact gttacttgtc tgaatcatcc agttcgtctg ttttcacatc ctcaaacctt
      421 ggatgcaggc gattcatttt tgcaataaaa tctgaatagt cgttagatag cttcataatc
      481 gtaacggttg atgacagatg ctctcttaat ttttgatatc caatatttgg cgtcagcccc
      541 tgaaacaact ttgtaccttt tgaggccttt acgttctgct ttttaagctc ctcaaggatg
      601 tttggtgcca atctcttgta gacgatatca tttgtcaaaa cgccaaaata ctgaggccgg
      661 aagcggggat tttccggcgg gtattctaag ccccttaacc tgaaaagctc ttcataataa
      721 tcagcaggaa atgttgtaat ataaggttga atttcctttg cgacaaaggc ctcgagtatt
      781 ttggcgagcg catctttttc tctatctcgc tggtaccccg tcgcttcatc aacaagcgct
      841 attattccga ctttagctaa tgagcgcacc agaatttcag ctttcttggc tgtctctaac
      901 tggtttggcc tggtgatagc gcctgcctct cttgccttta aataaacatc gcagacaaga
      961 ggtattatgg atgcgtcata accttcctgg acggagccag taatcgtctt gtatttgacc
     1021 ttattgatca catccataac atcttgattt atatattttt taaggtttgc agcatccata
     1081 aaagcgggca tattgatcac cccctcttct tgaggtgctc taccccctct ttgtggtcgg
     1141 ccaaatgctt taaaaacaga agcttgtgat atgatacgcc gtccattttc gagaaccgcg
     1201 acatctaatt ccgcatcacc gatctttaac tttccctcat tcgcagatac gggcaataaa
     1261 gctttttctt tttttgcggc aacagccttt cttgaggatt cttttctttg ctctgctgtc
     1321 atttttgccg cgcgtgcctt accgcctttg gccttcccac taacatcatc atttttcatg
     1381 agcatatctc gtgttgtgat tgataagtca atcatacaca tgcacgttgt aacgtgcaat
     1441 atttaaatat gcacgtaaat tgcgaatcag ataatgagga gactttctcc cccttgcact
     1501 gacatcatgg tattctgctc aaaactaaat ttctggagcg tttcgttgga aggtatttgc
     1561 agttttcgca gatgatgtcg gtgatgcttc tttgctgtcg cctcatgccg cccttctgac
     1621 gccctgcccg atcgccatca atgccgcttt ggatacggta gtaaacatcc gtcgaggact
     1681 gatgaacggt cgccaaatca gcagcatgga acctttgctg tttcccttct tctccagccc
     1741 tgtcgatggt tcgataaaat taatccgtcc atcagtgatg atgcgaactt cgtcaacact
     1801 ctccagagcc ttgctgaacc atccgactga catatcctct ggcacaagca t
//
LOCUS       DAZKHC020000291         1839 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZKHC020000291 DAZKHC020000000
VERSION     DAZKHC020000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.293"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            39..1232
                     /locus_tag="ACHOSM_RS25375"
                     /old_locus_tag="pgaptmp_005075"
     CDS             39..1232
                     /locus_tag="ACHOSM_RS25375"
                     /old_locus_tag="pgaptmp_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4561357.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGAAIALIMAALFATILFTIVSVFIWH
                     YRAIK"
     gene            1618..1839
                     /locus_tag="ACHOSM_RS25380"
                     /old_locus_tag="pgaptmp_005076"
                     /pseudo
     CDS             1618..1839
                     /locus_tag="ACHOSM_RS25380"
                     /old_locus_tag="pgaptmp_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1683..1799
                     /locus_tag="ACHOSM_RS25380"
                     /old_locus_tag="pgaptmp_005076"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          409 a          471 c          521 g          438 t
ORIGIN      
        1 aaataaacac aattaataca tggagttgaa acattaaaat gtcacctggg atcgaagata
       61 cgccccaaaa accactgtcc tgctggccac ttgcgttcag tgccgggctt ctcggtatcg
      121 ggcagaacgg tctgctggtt gtgctccctg ttctggtcat acagacaaat ctgagtctgt
      181 ctgtatgggc tgccctgctg atgcttggct caatgctgtt tctgccatcg tccccgtggt
      241 ggggaaagca aatttcccgt actggcagta aacctgtggt gttgtgggct ctgggaggat
      301 atggcataag ctttaccctg ctagggctgg gaagcgtgct gatggctacc agcgccataa
      361 caacagcggt gggactggga atattaatca tcgcccggat cgcctacggg ctgaccgtgt
      421 cggcaatggt gccagcctgt caggtctggg cattgcagag agcaggagaa gggaatcgca
      481 tggccgctct ggcaaccatc agctccggcc tgagttgcgg caggctattc gggccgctgt
      541 gcgcggcagc aatgctggcc attcatcctc tggcgccact ggggctgctg atggcagccc
      601 cagtgctggc gctgctgatg ctgctgcggt tgcccggcac accaccacag cccacaccgg
      661 agcgcaagag cgtcagtctg aagcgggatt gtctgcctta tctgctttgc gcaatattac
      721 tggctgcggc ggtgagcatg atgcagcttg gactttcgcc tgcccttact cgccagttcg
      781 ccacggatac caccgccatt agccaacagg tggcctggct gttgggtctg tccgcagtag
      841 ctgcgcttat cgcgcagttc ggggtactcc gtccgcagcg cctgactccg gtggccctgc
      901 tcctgagtgc cggagtgtta atgagtggtg gtctggctat catgctctcc gaacagctat
      961 ggttgtttta cccgggctgt gcagtgctgt catttggagc agctctggca acacccgctt
     1021 atcaacttct actgaatgat aagctggctg atggcgcagg cgcgggctgg ctcgctacca
     1081 gtcacacact tggctatgga ctgtgcgctt tgctggtgcc gctggtatcg aaaacaggtg
     1141 ccgcaatcgc gctgattatg gcggcattat ttgcaactat attatttacc attgtgtctg
     1201 tatttatctg gcattaccgt gctatcaaat aaagatatga tcatcacctt accggagtaa
     1261 atcagaggat atgagagaga acctgcgtct gatgaaagca tgtcaggcag taacactggc
     1321 aaaaatgcac cgcatggtac agggtatgtt cggtataaaa ccggccggtg tcacttaagt
     1381 tgcattgaag tcatgctggt tgtgagtgca acatcacatg gctctgtccg gatgcctgca
     1441 gacaatgaga acagtgcgat tactcattgg gaatgagatg aacgcatccc caacattaga
     1501 tctggggcac cgtgataaat aaaggggaaa atcagagagg tgtcgagcct cgcagatttc
     1561 cggcatcata gtgcccaata cgggatgcat aaaactctct cagagtggcg ttgacacctg
     1621 aaagagctgg agcgtgaaaa tcgtgaactg cgccgcagta acgatatcct tcgccaggct
     1681 tccgcttatt ttgcgaagga ggagttcgac cacctctgga aaaaatgatg ccactgctgg
     1741 ataagctgcg tgagcagtac ggggtcagac cggtatgcag cgaactgcat attgccccgt
     1801 caacgtatta ccattgtcag caacagcgac atcatccat
//
LOCUS       DAZKHC020000292         1835 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZKHC020000292 DAZKHC020000000
VERSION     DAZKHC020000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.294"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1835
                     /gene="rhsD"
                     /locus_tag="ACHOSM_RS25385"
                     /old_locus_tag="pgaptmp_005077"
                     /pseudo
     CDS             1..1835
                     /gene="rhsD"
                     /locus_tag="ACHOSM_RS25385"
                     /old_locus_tag="pgaptmp_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
BASE COUNT          413 a          537 c          636 g          249 t
ORIGIN      
        1 ggtgaactgc tggcggtata tgaccgcagc gggacgcagg tgcgcgcttt cacgtatgac
       61 ccgcaacacc cgggccggat ggtggcacac cgttacgcgg gaaggccgga tatgcgctac
      121 cgctacgacg ataccgggcg ggtggtggag ctgcttaacc cggcaggcct gagttaccgc
      181 taccagtatg agcaggaccg catcaccgtc accgacagcc tgaaccggcg tgaggtgctg
      241 catacagaag gcggggccgg gctgaagcgg gtggtgaaaa aagaactggc ggacggcagc
      301 gtcacgcaca gcgggtatga tgcggcagga aggctcacgg cgcagacgga cgcggcggga
      361 cggcggacag agtacggtct gaatgtggtg tccggcgata tcacggacat caccacaccg
      421 gacgggcggg agacgaaatt ttactataac gacgggaacc agctgacggc ggtggtgtac
      481 ccggacgggc tggagagccg ccgggaatat gatgaaccgg gcaggctggt atcggagaca
      541 tcgcgcagcg gggagacagt acgctaccgc tacgatgacg cgcacagtga gttaccggcg
      601 acgacaacgg atgcgacggg cagcacccgg cagatgacgt ggagccgcta cgggcagttg
      661 ctggcgttca ctgactgctc gggctaccag acccgctatg aatacgaccg cttcggccag
      721 atgacggcgg ttcaccgcga ggaaggtatc agcctttacc gccactatga caaccgtggc
      781 cggttaaccg gtgaaagacg cacagggccg tgaaacgcag tatgagtaca acgccgcagg
      841 cgacctgact gccgttatca ccccggacgg caaccggagc gagacacagt acgatgcgtg
      901 gggaaaggcg gtcagcacca cgcagggcgg gctgacgcgc agtatggagt acgatgctgc
      961 cggacgtgtc atcagtctga ctaacgagaa cggcagtcac agcgacttca gctacgatgc
     1021 gctggaccgg ctggtacagc agggcggctt tgacgggcgg acgcaacgtt atcattatga
     1081 cctgaccgga aaactcacgc agagtgagga tgaggggctt gtcaccctct ggcactacga
     1141 tgcgtcggac cgcatcacgc accggacggt gaacggcgac ccggcagagc agtggcagta
     1201 tgacggccac ggctggctga gagaaatcag ccacctgagc gaaggccacc gtgtcgctgt
     1261 ccactacggc tacgacgata agggccgcct gacgggcgaa cgccagacgg tggagaaccc
     1321 ggagacgggg gaactgctgt ggcagcatga gacaacgcac gcctacaacg aacaggggct
     1381 ggcaaaccgc gtcacgccgg acagcctgcc gccggtggag tggctgacct acggcagcgg
     1441 ttacctggcg ggcatgaagc tgggcgggac gccgctggtc gagtatacgc gggacaggct
     1501 gcaccgtgag acggtgcgca gcttcggcag catggcaggc agtaatgccg catatgaact
     1561 gaccagcaca tacacccccg caggccagtt acagagccag cacctgaaca gcctggtgta
     1621 tgaccgtgac tacgggtgga atgacaacgg cgacctggtg cgcatcagcg gcccgcgaca
     1681 gacgcgggaa tacggctaca gtgccacggg caggctggag agcgtgcgca ccctcgcacc
     1741 ggacctggac atccgcatcc cgtatgccac cgacccggcg ggaaaccggc tgccggaccc
     1801 ggagctacac ccggacagca cgctcacggc gtggc
//
LOCUS       DAZKHC020000293         1833 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZKHC020000293 DAZKHC020000000
VERSION     DAZKHC020000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.295"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..186
                     /locus_tag="ACHOSM_RS25390"
                     /old_locus_tag="pgaptmp_005078"
     CDS             <1..186
                     /locus_tag="ACHOSM_RS25390"
                     /old_locus_tag="pgaptmp_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4561358.1"
                     /translation="VSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGSLLFGLLTYLT
                     NLYFKIREDRRKAARGE"
     gene            190..747
                     /locus_tag="ACHOSM_RS25395"
                     /old_locus_tag="pgaptmp_005079"
     CDS             190..747
                     /locus_tag="ACHOSM_RS25395"
                     /old_locus_tag="pgaptmp_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561359.1"
                     /translation="MNHEEMNQRFSRLENEIAELNKKLSALMPSEDEKKRRDEQFAAF
                     DDYCRKVMSRNLAECFSIHNDNCSELEWECNRPSFVVSGDDGKITISENGKVTLPSHQ
                     HSETLIEFAIDYLKNNKKQGLMQRIGRCMGYLQVAAEIEALASGADKDAIVREALLRN
                     FNTPPFKKVPAYWLHPGLTYLKVRI"
     gene            784..1317
                     /locus_tag="ACHOSM_RS25400"
                     /old_locus_tag="pgaptmp_005080"
     CDS             784..1317
                     /locus_tag="ACHOSM_RS25400"
                     /old_locus_tag="pgaptmp_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4561360.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1616..>1833
                     /locus_tag="ACHOSM_RS25405"
                     /old_locus_tag="pgaptmp_005081"
     CDS             1616..>1833
                     /locus_tag="ACHOSM_RS25405"
                     /old_locus_tag="pgaptmp_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HGU4561361.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELA"
BASE COUNT          479 a          384 c          527 g          443 t
ORIGIN      
        1 gtgtcataca ccacgtcagc ggtgggaacg ggctactggt tcctgcagtt gctggacagg
       61 gtttccccgt ctcagtgggc ggcaataggc gtgctgggga gtctgctgtt tgggctgctg
      121 acatatctga cgaacctgta tttcaaaatc agagaggacc ggcgtaaggc ggcacgggga
      181 gagtaggtga tgaaccatga agaaatgaat cagcgcttca gtcgtctgga aaatgaaatt
      241 gctgaactga ataaaaaact gtcggcgctg atgccttctg aagatgaaaa aaaacgccgc
      301 gatgagcagt ttgctgcgtt tgacgattat tgtcggaaag tgatgagcag aaatctcgca
      361 gagtgtttca gtattcataa tgataattgc agtgagctgg aatgggagtg taaccggcca
      421 tcctttgttg tatccggtga tgacgggaaa atcaccatat cagaaaatgg aaaagtaaca
      481 ctgccatcgc accagcacag tgagacgctc attgaatttg ccattgatta cctgaagaac
      541 aataaaaaac aggggctgat gcagcgcatt ggtcgttgca tggggtatct gcaggtagcc
      601 gctgagattg aagcgctggc cagtggtgct gataaggatg caattgtgcg ggaggctctt
      661 cttcgtaatt ttaatactcc accctttaaa aaagtgccgg cttactggct tcatcccgga
      721 ctgacttatc tgaaagtgcg tatttagtgg gcctgggaca gcggctgaat atttaatata
      781 tccatgaaca caaaaatcaa atacggcctg tcggctgccg ttctggcgct gattgccgca
      841 ggtgcttctg cgcctgaaat tctcgaccag tttcttgacg aaaaagaagg taaccacacc
      901 acggcatacc gtgatggtgc gggtatctgg accatctgcc gtggagccac ccgggtggat
      961 ggtaagcctg ttattcctgg catgaagctg tcgaagggga aatgcgaccg ggttaacgcc
     1021 attgagcgtg ataaggcgct ggcatgggtg gagaaaaaca tcaaagtgcc actgactgaa
     1081 ccccagaaag cggggatcgc gtcattctgc ccgtataaca ttggccccgg taagtgtttc
     1141 ccgtcgacgt tttataaacg aattaatgca ggcgatcgca ggggagcgtg tgaagcgatt
     1201 cgctggtgga ttaaggacgg aggcagagac tgccgtatcc gctcaaataa ttgctatggt
     1261 caggtatccc gtcgtgacca ggagagcgcg ctggcgtgct ggggtatcga cagataagca
     1321 gaatattttg ctgaaaaatg acgttggcca acgcggacgg ataacacgaa atcctgcgaa
     1381 ctggcaaaac ctgagtgaat aaaagtaaaa accccgtttg ttagcagcag gtggggtttt
     1441 gtgtttcctg actccggaaa agtcaaagga gaaagtgtgt ttgattttag caaactgatt
     1501 cgggagattt gagtgatggc tgaaaaatta tctacctgga agttcattct tatctggctg
     1561 gtgtttgtga ttatggtctc cggttatttc atcggtcaga tacgctggtg gtgaaatgaa
     1621 ccgcgtactg tgtgtggtca tcattgccct gctggtggcc tgtggtgcgc ttagtctggg
     1681 gctgaatcat taccgtgata acgccatcac ctacaaagcg cagcgcgata aaaaagccag
     1741 agagctggag ctggcaaacg caaccattac tgatatgcag gtgcgccagc gcgatgttgc
     1801 tgcgctcgat gcaaaatact caagggaatt agc
//
LOCUS       DAZKHC020000294         1803 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZKHC020000294 DAZKHC020000000
VERSION     DAZKHC020000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.296"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(33..>1803)
                     /locus_tag="ACHOSM_RS25410"
                     /old_locus_tag="pgaptmp_005082"
     CDS             complement(33..>1803)
                     /locus_tag="ACHOSM_RS25410"
                     /old_locus_tag="pgaptmp_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4561362.1"
                     /translation="DRIQVSTLPDGVATYGVWGLSLPSLRRRLFRCVSIRENTDGTFA
                     ITAVQHVPEKEAIVDNGARFEPMSGSLNSVIPPAVQHLTAEVTADSGEYQVLARWDTP
                     KVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFRQLALGNYRLTVRAVNAWGQQG
                     DPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRITDIRQVE
                     TTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFF
                     KGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDANDKWNAMWGVKIEQTKDGKHY
                     VAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFMNDVFLKRLT
                     APTITSGGSPPAFSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCRVLGKLSANQ
                     IEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEREH
                     TDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWY
                     PTASISDLLVVVMKKSTAGISIS"
BASE COUNT          383 a          542 c          450 g          428 t
ORIGIN      
        1 catttctggc gcccgtatat gggttataaa attcagctga tactgatgcc tgctgtggat
       61 ttcttcatca ccacaaccag caaatcgctg atacttgctg tgggatacca gttattcacc
      121 agccatgctg ataccgaaaa ctccagcgtc atgtgaccgt gaccggcagg catatcaata
      181 acgccactgt aaatcagcgt attatccagc gcggtacggt tataaatttc agcaccgttt
      241 ttccgcacta tcagacggca tgaggagtaa atatcagtat gctctctctc atgcttagcg
      301 ccactgaatg ccaccgccgg aataacaatc tgccggtcaa acggctgatc gtcataaacc
      361 ctgacggtaa tggtccctga tggccaccgc tccggtgcac gggaatcccg ggggaaagct
      421 ttgcccactg ttttaacgag atcgccttca atctggttcg cggacagttt tcccagaacc
      481 cgacagttct cattaatcgt gacgttgttg agcgtcccgg agttcgcatt cacactacca
      541 ctgatatccg catttttagc agtcagcttt ccgtccggtg tcagggaaaa tgccggagga
      601 ctgccaccac tggtaatggt gggggccgtc aggcgcttca ggaacacgtc gttcatgaat
      661 atctggttgc cctgcgccac aaacatcgga gtttcattcc cgtttgccgg gtcaataaac
      721 gcgatacggt tagcggcaac cagaaactgg ctcagcttgc cttcctccgt gtcctccatg
      781 ctgaggccaa tacccgcgac ataatgtttg ccgtctttgg tctgctcaat tttgacgccc
      841 cacatggcat tccatttatc gttagcgtcc ttccactctt tcgaaaactc atccagtctg
      901 ctggcgttat cctccgtcag atcgactttt tccagcagct ccttgccgag atgagattcg
      961 gttatcttgc ctttgaaaaa atccagataa ccttccgcat catcgctcgc ccgaccgacg
     1021 gcctccacga atgccgattt gccaacagtg ttcacactgc ggatataaaa gtaataatca
     1081 tggcccggtt tgatattgat actggcggct atccagtaca gccccgtgcc aaggtagcgg
     1141 gctgtggttt caacctgcct gatatcggta atccgctttt ccgagaacca gaactcaaac
     1201 tgtaccgtcg ggtcataaac ggcaagatgc ggcgtggcgg ttatctgaaa atagcccggc
     1261 gtcagctcaa tcctcgacgg tgctgccggt gcggcaatcc ggaacgatac cgacgccgga
     1321 tcaccctgct gcccccacgc atttaccgcc cggactgtca gcctgtagtt ccccagcgcc
     1381 agttgcctga agcggtatgt ggtttccgtc gtccgggccg tgctgaccag ccgctcactg
     1441 ccgtcatccg ctgttacggt cagacggagc aggaagctca cgcccttcac caccttcggc
     1501 gtgtcccagc gcgccagcac ctgatattcc ccgctgtctg cggtgacttc ggcagtcagg
     1561 tgctgcactg ccggcgggat gacgctgttc agtgagccgg acatcggctc aaagcgggcc
     1621 ccgttatcca caatggcttc tttttccggc acatgctgca ccgccgtgat ggcaaaggtg
     1681 ccgtccgtgt tttcccggat ggaaacacag cggaacaggc gacgacgcag tgacggcagg
     1741 gagagtcccc acacaccgta tgtcgccacg ccatccggca gggtactgac ctgtatccgg
     1801 tcc
//
LOCUS       DAZKHC020000295         1796 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZKHC020000295 DAZKHC020000000
VERSION     DAZKHC020000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.297"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            420..578
                     /locus_tag="ACHOSM_RS25415"
                     /old_locus_tag="pgaptmp_005083"
                     /pseudo
     CDS             420..578
                     /locus_tag="ACHOSM_RS25415"
                     /old_locus_tag="pgaptmp_005083"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            565..630
                     /locus_tag="ACHOSM_RS25420"
                     /old_locus_tag="pgaptmp_005084"
     CDS             565..630
                     /locus_tag="ACHOSM_RS25420"
                     /old_locus_tag="pgaptmp_005084"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HGU4561363.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            746..1003
                     /locus_tag="ACHOSM_RS25425"
                     /old_locus_tag="pgaptmp_005085"
     CDS             746..1003
                     /locus_tag="ACHOSM_RS25425"
                     /old_locus_tag="pgaptmp_005085"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4561364.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDAHF"
     gene            complement(1050..1619)
                     /gene="nleF"
                     /locus_tag="ACHOSM_RS25430"
                     /old_locus_tag="pgaptmp_005086"
     CDS             complement(1050..1619)
                     /gene="nleF"
                     /locus_tag="ACHOSM_RS25430"
                     /old_locus_tag="pgaptmp_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HGU4561365.1"
                     /translation="MLPTSGSSANLYSWMYVSGRGNPSTPESVSELNHNHFLSPELQD
                     KLDVMVSIYSCARNNNELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALRAGM
                     TIEDRDSYIRDLFFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAMKCL
                     VEGCSDDFNPFDIIRVPDLTYNKGSLQCG"
     gene            complement(1685..>1796)
                     /locus_tag="ACHOSM_RS25435"
                     /old_locus_tag="pgaptmp_005087"
     CDS             complement(1685..>1796)
                     /locus_tag="ACHOSM_RS25435"
                     /old_locus_tag="pgaptmp_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector protein NleH"
                     /protein_id="HGU4561366.1"
                     /translation="DISSYNVSDISWSEHQVMQSYHGGKLDLISVVLSKI"
BASE COUNT          547 a          405 c          340 g          504 t
ORIGIN      
        1 gattttctgc gcttctgcaa ataatcaagg ggttacaaag tggttgcccc tttgttttta
       61 gtgctcttgg tatacccatt ggtacattta gcgcaatacc atctggtatc acttaaagac
      121 acgaaaaaca actttttatc tttttgtttc gctcaaatta gtataaaaag ccgaacaaca
      181 aacaataaaa aaccattaac atcaatagat tatgatggct tcaatctaaa taataggcta
      241 tataatactg caagacacaa cacatgctgt cactatgagt caactattta gatgatatta
      301 gtgacctgtt tcagagcatt agcgcaaggc gattctttgt cttcttgcac taattttttg
      361 tcataaaaat gttcctagca ctgggcatca atatcgcagg tcagaaagag ctcctgggga
      421 tgcggctggc cgaaaatgaa ggggcgaatt tctggttcaa tgtgctgact gaactgaaaa
      481 accgcggtct gaacgatatc ctcatcgcct gtgtggatgg cctgaaagaa ttcccggagg
      541 cccgcatcca gttatgcatc gtgcatatgg tgcgcaacag catgcgcttc gtgtcatgga
      601 aggaatacaa agccgtcact cgcgacctga aagcgattag cctcccacag aagtggcagg
      661 ccagcaggca ctggaagcgt ttgctgcggc ctgggactgc cgctatccgc agataagccg
      721 gtgctagctg tcaaactgga ctaacttggc gacgtttttc gcttatccgg cagatatccg
      781 caaagtgatc tacacaacga acgccatcga gtcgctgaac agtgtgatcc ggcatgccat
      841 caggaaacgc aaggtgttcc cgacggacga gtcagtaaaa aaagtggtgt ggctggcaat
      901 ccaggccgcg tcacagaaat ggacaatgcc gttaagggac tggcgaatgg caatgagccg
      961 ctttattatc gagtttggtg accgtcttga cgctcacttt taagaaaagg catttacaca
     1021 gaatcctaaa caggctcaca ggcctctaat catccacatt gtaaagatcc tttgttgtaa
     1081 gtaagatctg gtaccctaat aatatcaaaa ggattaaaat catcactgca tccttccacg
     1141 aggcatttca ttgctcgtag atcaccatat agctctgaag agtgatgcgg acatagcaga
     1201 ttataaactt tacatttcga atcttctttg ccttgtctac tttcctcaat ttttactttc
     1261 aatgaatgca gaaaaaaaag atccctgata taactatccc tatcctctat cgtcattccc
     1321 gccctcagcg ctccgacaac ctcatcagta ttttcatttc tcacctcaaa tacactattt
     1381 ctcttatcca tcagcccact tacaaaagca cttagctctt gaaaaatttc ctcaagctca
     1441 ttattatttc tggcacatga atatatagag accataacat caagtttatc ttgtaattca
     1501 ggagaaagaa agtgattatg attaagctca cttactgatt ccggagtcga agggttacct
     1561 cttcctgata catacatcca tgaataaaga tttgctgaag aaccacttgt tggtaacata
     1621 tcaaaacccc cttaacaaat aaatccataa acattattgc aactaatata tctggataaa
     1681 aatattatat cttacttaat actacactaa taagatccag ctttcctccg tgataagatt
     1741 gcatgacttg atgttcactc catgaaatat cagaaacatt ataagatgat atatct
//
LOCUS       DAZKHC020000296         1779 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZKHC020000296 DAZKHC020000000
VERSION     DAZKHC020000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.298"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..264
                     /locus_tag="ACHOSM_RS25440"
                     /old_locus_tag="pgaptmp_005088"
     CDS             <1..264
                     /locus_tag="ACHOSM_RS25440"
                     /old_locus_tag="pgaptmp_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4561367.1"
                     /translation="TEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAAL
                     RISISRWQLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            261..467
                     /locus_tag="ACHOSM_RS25445"
                     /old_locus_tag="pgaptmp_005089"
     CDS             261..467
                     /locus_tag="ACHOSM_RS25445"
                     /old_locus_tag="pgaptmp_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HGU4561368.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            464..>1779
                     /locus_tag="ACHOSM_RS25450"
                     /old_locus_tag="pgaptmp_005090"
     CDS             464..>1779
                     /locus_tag="ACHOSM_RS25450"
                     /old_locus_tag="pgaptmp_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4561369.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTR
                     PSRLFRTQFRMVSPKRISNPNNTSDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYSAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEAR"
BASE COUNT          405 a          465 c          560 g          349 t
ORIGIN      
        1 accgaagcgc agcaactgac tgctgaagag caggtcgaaa aatgggtgga tggcaggaaa
       61 aaaatactgt gggacagcaa aaagcgacgc aatgaggcgc tcgactgctt cgtttatgcg
      121 ctggcggcgc tgcgcatcag tatttcccgc tggcagctgg atctcagtgc actgctggcg
      181 agcctgcagg aagaggatgg tgcagcaacc aacaagaaaa cactggcaga atacgcccgt
      241 gccttatccg gagaggatga atgacgcgac aggaagaact tgccgctgcc cgtgcggcac
      301 tgcatgacct gatgacagga aaacgggtgg caacggtaca gaaagacgga cggcgagtgg
      361 agtttacgac cacttccgtg tctgacctga aaaaatacat tgctgagctg gaagtgcaga
      421 ccggcatgac acagcgacgc aggggaccag caggatttta tgtatgaaaa tgtccaccat
      481 tcccaccctt ctggggccgg acggcatgac atcgctgcgt gaatatgccg gttatcacgg
      541 cggtggcagc ggatttggtg ggcagttgcg ggcgtggaac ccaccgggtg aaagtgtgga
      601 tgcagccctg ctgcccaact ttacccgtgg caatgcccgc gcagacgatc tggtacgcaa
      661 taacggctat gccgccaacg ccatccagtt gcatcaggat catatcgtcg ggtctttttt
      721 ccgactcagt catcgcccaa gctggcgcta tctgggcatc ggggaggaag aagcccgtgc
      781 cttttcccgc gaggttgaag cggcatggaa agagtttgcc gaagatgact gttgctgcat
      841 tgacgttgag cgaaaacgca cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc
      901 ctttaacggt gaactgttcg ttcaggccac ctgggatacc cgtccctcgc gactgttccg
      961 gacacagttc cggatggtca gcccgaagcg catcagcaac ccgaacaata ccagcgacag
     1021 ccggaactgc cgtgccggtg tgcagattaa tgacagcggt gcggcgctgg gatattacgt
     1081 cagcgaggac gggtatcctg gctggatgcc gcagaaatgg acatggatac cccgcgagtt
     1141 acccggcggt cgtgcttcgt tcattcacgt ctttgaaccc gtggaggacg ggcagacccg
     1201 cggtgcaaat gtgttttaca gcgtgatgga gcagatgaag atgctcgaca cgctgcagaa
     1261 cacgcagctg cagagcgcca ttgtgaaggc gatgtatgcc gccaccattg agagtgagct
     1321 ggatacgcag tcagcgatgg attttattct gggcgcgaac agtcaggagc agcgggaaag
     1381 gctgaccggc tggattggtg aaattgccgc gtattactcc gcagcaccgg tccgtctggg
     1441 aggcgcaaaa gtgccgcacc tgatgccggg ggactcactg aacctgcaga cggctcagga
     1501 cacggataac ggctactccg tgtttgagca gtcactgctg cggtatatcg ctgccgggct
     1561 gggtgtctcg tatgagcagc tttcccggaa ttacgcccag atgagctact ccacggcacg
     1621 ggccagtgcg aacgagtcgt gggcgtactt tatggggcgg cgaaaattcg tcgcatcccg
     1681 tcaggcgagc cagatgtttc tgtgctggct ggaagaggcc atcgttcgcc gcgtggtgac
     1741 gttaccttca aaagcgcgct tcagctttca ggaagcccg
//
LOCUS       DAZKHC020000297         1776 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZKHC020000297 DAZKHC020000000
VERSION     DAZKHC020000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.299"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1604
                     /locus_tag="ACHOSM_RS25455"
                     /old_locus_tag="pgaptmp_005091"
                     /pseudo
     CDS             1..1604
                     /locus_tag="ACHOSM_RS25455"
                     /old_locus_tag="pgaptmp_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1,RefSeq:WP_005148713.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
BASE COUNT          394 a          459 c          559 g          364 t
ORIGIN      
        1 ctatgatgaa tacggcagac tgacggagaa gacggaccgc atcccgacgg gtgtgatacg
       61 gacgacgagc ggacccacca ctaccactac gacagccagc accgcctggt gttccacacg
      121 cggatacagc atggcgagcc actggtcgag agccgctacc tctacgaccc gctgggacgg
      181 cgaatggcga aacgggtatg gcggcgggag cgtgacctga cggggtggat gtcgctgtcg
      241 cgtaaaccgg aggagacatg gtacggctgg gacggcgaca ggctgacgac ggtacagacc
      301 gacaccacgc gtatccagac ggtaggtgcg ccgacgggag tggcctgttc ggtgtgtccg
      361 ggagggatta cctatgctaa cccggtgaac ccggtgctgg gtgcgaaggt gctgccgggc
      421 gagacggacc ttgcgctgcc ctgcccgctg ccgtttattc ttttccgcgc ctacagcagc
      481 taccggacca gaacacccgc gccggtgggg gtgtttggtc ccggctggaa agcgcccttc
      541 gatatccgct tacagatacg cgatgaaggc ctgatactca acgacagcgg cggcaggagc
      601 attcactttg agccgctgtt tcccggcgag ataagctaca gccgcagcga gtcactctgg
      661 ctggcgcggg gcggagtggc ggcgcagcac agttcgcagc cgctaagcgc gctctggcag
      721 gtgctgccgg aagatgttcg cctgagtccg catgtgtacc tggcgacaaa cagccttcag
      781 gggccgtggt ggatactcag ctggccggag cgggtgccgg gggcggacga ggtgctgccg
      841 ccggagccgc ccgcataccg ggtgctgacg ggcgtggtgg acggcttcgg gcggacgctg
      901 gcctttcacc gggcagcgga gggtgacgtg gcgggcgcgg tgacgggggt gacggacggc
      961 gcgggccgcc gttttcacct ggtgctgacc acacaggcgc agcgggcgga agaggcccgt
     1021 aaaccgcaca ccgcttcgtt atcttcccct gacagtcccc gccctctctc agctccttcc
     1081 ttccccgaca cgctgcccgc cggtacagag tacggtgccg atatccactg gcaccaatac
     1141 gttacactga tccactgggg ttagaacgag ttataagtgt atatgggcct cccgctccag
     1201 accgggcagg tgctgaaact cctcttgttc ttactgatat gactggagga gtgactattt
     1261 attatgatcc tgaaacaggt gattcaatga cttttgatag tagcaataga attgatcgtc
     1321 gttctcaacg tggtgctggt gatccatata caggtgaagt cgttggttgt gagacaaatg
     1381 gatcaggaat atcggcagca tatggcacaa cgaagatcta tactacagat actagagcaa
     1441 gatggctaca tggtggggga tcatcgttgc gcgatcctta tgctcctcga caaggctgga
     1501 aaccaactat gggatgtacc cgtgcacaga atgaagatgt agatgagttg tgtaagaaag
     1561 tgacaagttg gatgtattcg catcctggaa agagaattag atatgaacgt tttaagacac
     1621 gttaatgcga ttaacatggg agtgtgcatt ctaattttgg ttattggatg tgcaaatgct
     1681 aaagttctta aacgatgtat attacccgct cctgatggtg gcgtagaaaa actatcaaaa
     1741 cccaacttac atggagtaat taaaaaagtt gatttg
//
LOCUS       DAZKHC020000298         1748 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZKHC020000298 DAZKHC020000000
VERSION     DAZKHC020000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.300"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..348)
                     /locus_tag="ACHOSM_RS25460"
                     /old_locus_tag="pgaptmp_005092"
     CDS             complement(<1..348)
                     /locus_tag="ACHOSM_RS25460"
                     /old_locus_tag="pgaptmp_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG"
                     /protein_id="HGU4561370.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEA"
     gene            complement(461..730)
                     /locus_tag="ACHOSM_RS25465"
                     /old_locus_tag="pgaptmp_005093"
     CDS             complement(461..730)
                     /locus_tag="ACHOSM_RS25465"
                     /old_locus_tag="pgaptmp_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4561371.1"
                     /translation="MNILRKLMERLCGCGKHDDCEHGQSLTVQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
     gene            complement(732..>1748)
                     /locus_tag="ACHOSM_RS25470"
                     /old_locus_tag="pgaptmp_005094"
     CDS             complement(732..>1748)
                     /locus_tag="ACHOSM_RS25470"
                     /old_locus_tag="pgaptmp_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4561372.1"
                     /translation="PEALRRFELMVEEVARHAEEAKKNAGEAETSARNAGISASQAEE
                     SAANADTSAGEASESARQAAESAAAAKKSEEASSSSASAAAQKASESSQSAAEAELSR
                     KTAESAAGNAARDATTATEKARESAESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGEQ
                     GPAGPQGPKGDKGERGDTGPVGATGERGPAGDAGPAGPQGPKGDRGERGETGLTGNAG
                     PQGPKGDTGAAGPAGPQGPKGETGAAGPVGATGPQGPKGDPGETQIRFRLGPASIIET
                     NSHGWFPDTDGALITGLTFLDPKDATRVQGFFQHLQVRFGDGPWQDVKGLDEVGSDTG
                     RTGE"
BASE COUNT          337 a          583 c          380 g          448 t
ORIGIN      
        1 agcctcaagc gggcactgaa gtctctctgg acaaacagaa aacttacatt gagatatttt
       61 gttctgaata ctctcttgcg attgtgcttc acctgagtcc gaaagcatac ttagcattct
      121 ctcaccaagg gctcgaggac cactattaaa tcccaaaagc aataactcag aaagcaatcc
      181 gccattaccg ccactggatg aaaagcgccc attagtagaa tcatacacaa tactgacagt
      241 ttcaccaccg actgtaatat ctccgcgccc attggctaca attcgggcac gaacaacctc
      301 caccccaaga ttaaatgaat gtgatctaat atctgaggtt aatggcataa caaagctccc
      361 tattttaatt tgaactccag acttaaatag ctgtaacaaa catcccccat acgtgacaac
      421 aaaaaccaga gcaggactcc ggtttttgtg aacccgtcgg ctatttcatc ccgccaatat
      481 tttcccacct cccgtcagca cgcaggattt gcagcggtct taccacgcac tgtatctgct
      541 ttttatccgc atccagtatc accacctgtg tgattaccct gtcctgctcc ggaataatgc
      601 cattctcatc tgactccagg atgtctgccg gccccagtcg cagttgtact gtaagcgact
      661 gcccgtgttc acagtcatca tgctttccgc aaccacacag acgctccata agctttctca
      721 aaatattcat gtcattctcc tgttctgcct gtatcactgc ccacttcatc cagtccctta
      781 acatcctgcc acggcccgtc gccaaacctg acctgcaaat gctgaaaaaa accctgaacc
      841 cgtgtggcat ctttggggtc aagaaaggtc agtccggtga tgagtgcgcc atctgtatcc
      901 gggaaccagc catggctgtt tgtctcaata atgctcgccg gccccagacg aaaacggatt
      961 tgtgtctccc ccgggtcgcc cttcggtccc tgaggtccgg ttgcccccac cgggccagcc
     1021 gcacctgttt ctcctttcgg tccctgtggg ccagccgggc ctgccgcacc ggtatctccc
     1081 tttggaccct gtggacctgc atttcccgtc agaccggtct ctccccgctc tcccctgtcg
     1141 cctttcggtc cctgtgggcc tgccggacca gcatcacctg ccggtccccg ttcgccggtt
     1201 gccccgacag ggccggtgtc accacgctct cccttatcac ccttcggccc ctgagggcca
     1261 gcgggcccct gttccccctt tggcccggga ggtcccacca cggtggggat tcggtttacg
     1321 gcctcttccg ccgctatcct gctttgttcc gctgactgtg cgctttctgc tgactcccgg
     1381 gctttttctg ttgcggtcgt tgcatccctg gctgcattac cggctgcact ttctgccgtc
     1441 tttcttgaca attcagcttc tgctgcactt tgtgatgact cactggcttt ttgagcggcc
     1501 gcagaagccg aggacgagga cgcttcctct gacttctttg cagcggctgc actttctgcc
     1561 gcctgccggg ctgactccga tgcctcccct gctgaagtgt cagcatttgc agcgctctct
     1621 tctgcctgac tggctgatat gccggcattc ctcgctgacg tctccgcctc tccggcattc
     1681 ttcttcgcct cctcagcgtg acgcgccacc tcttccacca tcagttcaaa acgacgcagt
     1741 gcctccgg
//
LOCUS       DAZKHC020000299         1726 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZKHC020000299 DAZKHC020000000
VERSION     DAZKHC020000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.301"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..300
                     /locus_tag="ACHOSM_RS25475"
                     /old_locus_tag="pgaptmp_005095"
     CDS             <1..300
                     /locus_tag="ACHOSM_RS25475"
                     /old_locus_tag="pgaptmp_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU4561373.1"
                     /translation="GQNLYLDNMAVTGFYRVPLSSAQAGDILLCCFGASVANHAAIYC
                     GNGELLHHLPEQLSKRERYSEKWQRRTHSVWRHRHWHASAFTGIYNDLAAASACT"
     gene            297..878
                     /locus_tag="ACHOSM_RS25480"
                     /old_locus_tag="pgaptmp_005096"
     CDS             297..878
                     /locus_tag="ACHOSM_RS25480"
                     /old_locus_tag="pgaptmp_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4561374.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMTEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGTALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKVPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQG"
     gene            complement(1066..1593)
                     /gene="sodC"
                     /locus_tag="ACHOSM_RS25485"
                     /old_locus_tag="pgaptmp_005097"
     CDS             complement(1066..1593)
                     /gene="sodC"
                     /locus_tag="ACHOSM_RS25485"
                     /old_locus_tag="pgaptmp_005097"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGU4561375.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPVLKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
BASE COUNT          391 a          436 c          496 g          403 t
ORIGIN      
        1 ggtcagaacc tttacctgga caatatggcg gtcaccggct tttaccgggt gcccctgtcc
       61 tctgcacagg cgggcgatat cctgctgtgc tgctttggcg catcggtggc caatcatgcc
      121 gccatttact gcggcaacgg tgaactgctt caccatctgc ctgaacaact gagtaaacgg
      181 gagaggtatt ccgaaaaatg gcaacgacga acgcattctg tctggcgtca ccgccactgg
      241 cacgcatctg ccttcacggg gatttacaac gatttggccg ccgcctcagc ctgtacgtga
      301 acacggcagc ggaagccatt cgcgccctgt cgatgcagat gccgggcttt cgccgtcaga
      361 tgaccgaagg ctggtaccag atacgtattg ccggtgatga cacggcaccg gaggcggtgt
      421 acgcccgtct tcacgaacag ctgggtgagg gaacggtcat ccacattgtg ccgcgactgg
      481 ccggggccgg aaagggtgga ctgcagattg tgctgggggc ggcagccatc gtgggctctt
      541 tcttcactgc cggtgcttcg atggcgttat ggggtacagc cctgagtgcc ggcggttttt
      601 ctgccaccac gatgctgttt tcacttgggg ccagcatgat tctgggcggt gtggcccaga
      661 tgctggcccc gaaggccaaa gtaccggagt acaaaagcac ggataacggt aaacagaaca
      721 cgtacttttc ctcactggac aacatgattg ctcaggggaa cccgatgccg gtgccttacg
      781 gtgaaatgct ggttggctca cggcgaatct cccaggacat cagtacccgt gatgaaggcg
      841 gtgacgggaa ggtggtggtt atcgggcggc aggggtaaaa agaataaaaa aatcccgcag
      901 agttgcggaa ctgcgggagc gttacgaaga ttgagtgtaa ggaattattc ttatgtcacg
      961 acaaaaacat taactcagag agggaggatg tgccgttctt ttcagggaga aaggatttat
     1021 cgtcctgagg aataaaggta aggggcccgc cccttacctg actgattatt gaatgatgcc
     1081 gcaggccatt ctcgcaccac caccgcccag gggctccgga tggtcatgat ggttatcacc
     1141 gccagcatga agcatgagag aacgcccttt aatctctttt aatgagttca gtctcggggc
     1201 caggaccggg tagttcgctt ttccgtcatg cgtcacgaac agcgcaggga ggtcgcccag
     1261 gtgtccatcc ggagaccagg ggccaagatg tttgccggtg tttttcgggt caaagtgacc
     1321 gccagccgat aatgctgcga ccggttttcc gtctttcagt accggggcgc aatttccttt
     1381 ttcgtgcaca tgaaaaccat gaatgccttc agacagagag tgaagggctg gtgtgaacag
     1441 cagaccgtag ggggtctcct gaatggttat ttttccaatg ctgacttctt ttccgtcagc
     1501 actgacaagg ttcattggga cttcctgttc tgctgcgtat ccgcatgatg ctgctgtcag
     1561 catggcaatg gcagcaatga ttttacattt cataaaaccc tcattaattc cgttaacaga
     1621 ctgagcttgc tggttacagg gtaacaaaca gcgttctgat gatatcgcgc aatagctgtg
     1681 caatatccta tcactgcgat taataatacc aattgagagg aacatt
//
LOCUS       DAZKHC020000300         1674 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZKHC020000300 DAZKHC020000000
VERSION     DAZKHC020000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.302"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..192
                     /locus_tag="ACHOSM_RS25490"
                     /old_locus_tag="pgaptmp_005098"
     CDS             <1..192
                     /locus_tag="ACHOSM_RS25490"
                     /old_locus_tag="pgaptmp_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HGU4561376.1"
                     /translation="DEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMF
                     TTVSKAALMAIGQGVRRAA"
     gene            189..371
                     /locus_tag="ACHOSM_RS25495"
                     /old_locus_tag="pgaptmp_005099"
     CDS             189..371
                     /locus_tag="ACHOSM_RS25495"
                     /old_locus_tag="pgaptmp_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU4561377.1"
                     /translation="MRRQQRSITDIICENCKYLPTKRSRNKRKLIPKKSDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            646..1380
                     /locus_tag="ACHOSM_RS25500"
                     /old_locus_tag="pgaptmp_005100"
     CDS             646..1380
                     /locus_tag="ACHOSM_RS25500"
                     /old_locus_tag="pgaptmp_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HGU4561378.1"
                     /translation="MTTQISVETLSPITHNQIPVITTELLAQLYGTEPVRIRQNHHEN
                     KVRFVEGKHFFKVVGNDLKELRVALNYSQNLRVTLSNSQNLQPSLRGLQISPKARSLI
                     LWTERGAARHAKMLETDQAWDVFEKLEDCYFSQCEKNTGKQEKKLNGLSAKETDSLVW
                     LWDYANRSQALFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTRDVDIN
                     EPDGPTNLSAWVRLKNKELPPSLHRY"
     gene            1455..>1674
                     /locus_tag="ACHOSM_RS25505"
                     /old_locus_tag="pgaptmp_005101"
     CDS             1455..>1674
                     /locus_tag="ACHOSM_RS25505"
                     /old_locus_tag="pgaptmp_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4561379.1"
                     /translation="MNELINSNVIKMTSIEISELTGKRHDNVKRTIEMLAKNGVIRHP
                     QIEDCGRINGLGLNQSFRVYVFEGEQGKR"
BASE COUNT          498 a          374 c          401 g          401 t
ORIGIN      
        1 gacgaagttc gtattatcac tgatggacgg attaatttta tcgaaccatc gacagggctg
       61 gagaagaagg gaaacagcaa aggctccatg ctgctgattt ggcgaccgtt catcagtcct
      121 cgacgaatgt ttactactgt atccaaagcg gcattgatgg cgatcgggca gggcgtcagg
      181 agggcggcat gaggcgacag caaagaagca tcaccgacat catctgcgaa aactgcaaat
      241 accttccaac gaaacgctcc agaaataaac gcaagctaat cccaaaaaaa tctgacgtaa
      301 aaaccttcaa ctacacggct catctgtggg atatccggtg gctaagacat cgtgcgagga
      361 aaacaaggtg attgacgcga tgatttattc ggggctatat tcctcacgcg ccagcaaaat
      421 ctggcgtcgg gattggcgtc ctggatagag accgcgacag atacacgccg cgagcgtgtt
      481 ttttattgtc gtatgcacgc gcacatctga attatggtgg gctgtgtggg ggcggagaga
      541 tccgcgccgg tcggtttccc ggttacgcca accctgcaca gttcaccacc agacgattgg
      601 cgtcgtcggt ggtgagttat taagaaacca ccagagggcg tcattatgac aactcaaatt
      661 tctgttgaaa ctctctcccc gatcacccat aaccagattc ctgttattac caccgaactt
      721 ttggcgcagc tttacggcac tgagccggtg cgtattcgcc agaatcatca tgagaacaaa
      781 gtacgcttcg ttgaagggaa acactttttc aaagttgttg gtaatgacct taaagaattg
      841 cgggtagctt taaactactc acaaaatttg cgggttactt taagtaactc acaaaatttg
      901 caaccatctt taagagggtt acaaatttcc ccgaaagccc gctccctcat actctggaca
      961 gaacgcggag cagcccgtca tgccaaaatg ctcgaaactg atcaggcgtg ggatgtgttc
     1021 gaaaaactgg aagactgcta tttcagccag tgcgagaaaa atactggcaa acaagagaag
     1081 aagctcaacg ggctttccgc aaaagaaaca gacagccttg tatggctgtg ggattatgct
     1141 aaccgctcac aggcattatt ccgcgaattg tatccagcgc taaagcaaat tcaatcgaac
     1201 tattccggca gatgttacga ctacggtcat gagttctcgt atgttattgg aatggcgaga
     1261 gacgttttaa tcaatcacac acgagatgtt gatattaatg agccagacgg accaacgaat
     1321 ctttccgcat gggtaaggct taagaacaaa gaattacctc cttcactaca tcgctactga
     1381 caattgacaa cttaacaaac ccagcttcgg ctgggttttt tattggtgaa ttttcaatat
     1441 gagaggacat gacaatgaac gagctgataa atagcaacgt catcaaaatg accagcattg
     1501 aaatctctga gcttacaggt aagcgtcatg acaatgtgaa acgtactatc gaaatgctgg
     1561 ctaaaaatgg tgttatccga catcctcaaa ttgaggattg tggaagaatc aatgggttag
     1621 gcttaaatca aagttttcgt gtgtatgtat tcgaaggcga acaaggtaag cgag
//
LOCUS       DAZKHC020000301         1673 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZKHC020000301 DAZKHC020000000
VERSION     DAZKHC020000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.303"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..>1673
                     /locus_tag="ACHOSM_RS25510"
                     /old_locus_tag="pgaptmp_005102"
     CDS             3..>1673
                     /locus_tag="ACHOSM_RS25510"
                     /old_locus_tag="pgaptmp_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561380.1"
                     /translation="MIPNSTSNKGYVCIDMQCSSTSEPTASTSSNRSIKLAASTNVYP
                     ITRNDSELTLNDFLDNSSSTSSLDYINELGSQLTLNDFLDNIKTNEVDRTCTDVVINI
                     PQEIQTNTQENDLLLSDKNNSICIEIDERITKILTCKQKYQLDSIIHEIIPKENESAE
                     TVLHLMRVLNDQYHQVYNQSGCFYKAYMAIHNKIEQILPYAFRAGGGISIHLLIQALF
                     FNGDYNKSPSQSQQPSLYTSPSPTINTEAFLSNVLSLDITQVRILGDLLSATLFHAPT
                     IFYQYPKLIDEVKYCISNKKITGSVIARFTLCLTSTLLTMSPLLMLNGAVKTGSIVRT
                     IGRGVSYADIPLALAILGDSWYKAYKHGSSDNPNSAQRFISQEAAFKTTQRVLTQGLS
                     LMSSLSGAIMRSLEKGTPPQMMSLFIVNILNLLFHQNPYEGASASANALKRSTYSHNP
                     DILNTQAIALCVDLQHTKNITMPLFKTKDRISYAFNGQRTSPEDQKQILKEVINSCTQ
                     GERAILNTSQSETCKHKIDEIYEKRFSETELNTLPNEMKNFLIFLNKTH"
BASE COUNT          627 a          328 c          256 g          462 t
ORIGIN      
        1 acttgattcc aaattcaact tcaaataaag gttatgtatg cattgacatg cagtgttcgt
       61 caacctctga accaacagct tctacctcca gcaaccggag tattaaatta gccgcttcca
      121 caaatgtata tccgataaca agaaacgact ccgagctcac tctgaacgat tttcttgata
      181 atagctcttc tacgtcatca ttggactaca ttaatgaatt gggttctcaa ctgacgttaa
      241 atgattttct tgacaacata aagacaaatg aggtggatag aacatgtacg gatgtggtaa
      301 ttaatatccc acaagagata caaacaaata cacaggaaaa tgatttgtta ttatccgata
      361 aaaataattc aatatgcatt gaaatcgatg aaagaattac aaaaatccta acatgcaagc
      421 aaaaatatca actggacagc atcattcatg aaattatacc aaaagagaat gagagtgcag
      481 aaactgttct ccatcttatg agagttctga acgatcaata tcatcaggta tataatcagt
      541 caggatgttt ttataaagcc tatatggcca tacacaataa aatcgaacag atacttccat
      601 atgcgtttag agccggaggc ggaatcagca ttcacttgct catacaggca ttatttttta
      661 atggcgacta taacaaatca ccttcacagt ctcaacaacc atctttatat acatcacctt
      721 ctccaacaat aaatacagaa gcattcttaa gtaatgtatt atcactagat ataacccagg
      781 tacgcatact tggtgattta ctatcagcaa ctttatttca tgcaccaaca atattctatc
      841 aatatcctaa actaatagat gaagttaagt attgtataag taataaaaaa ataacaggtt
      901 cggttatagc acgatttact ctatgtttaa caagtacatt actaaccatg tcaccactgt
      961 taatgcttaa tggagcagtt aaaacaggta gcatagtaag aactataggt aggggagtga
     1021 gttatgctga tataccattg gccttagcta tattaggtga ctcatggtat aaagcttata
     1081 aacatggttc ttctgataac ccaaattctg ctcagagatt tatatcgcaa gaagcggcct
     1141 ttaaaaccac ccagcgggta ttaacacaag gattaagtct gatgtcctct ttatcgggag
     1201 caatcatgcg ctctcttgag aaaggcacac caccacaaat gatgtcttta ttcatcgtaa
     1261 acatactaaa tctattattt catcaaaatc catatgaagg ggcatcagca agtgctaatg
     1321 ctttgaaaag atcaacttac tcccataatc cggacatact aaatactcag gcaatagctc
     1381 tttgtgttga cctccagcat acaaaaaata taaccatgcc acttttcaaa acaaaagaca
     1441 ggatatctta cgcattcaat ggacaaagaa catccccaga agaccaaaaa caaatactga
     1501 aagaagttat aaactcctgt acccaaggag aaagagccat tttaaataca tcacaatcag
     1561 aaacatgcaa acataaaatc gatgagattt atgaaaaaag attctcagaa acagaactaa
     1621 atacattacc aaacgaaatg aaaaatttct tgatattttt gaacaaaact cat
//
LOCUS       DAZKHC020000302         1652 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZKHC020000302 DAZKHC020000000
VERSION     DAZKHC020000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.304"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..306
                     /locus_tag="ACHOSM_RS25515"
                     /old_locus_tag="pgaptmp_005103"
     CDS             <1..306
                     /locus_tag="ACHOSM_RS25515"
                     /old_locus_tag="pgaptmp_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561381.1"
                     /translation="VDSFDYPRLQALVSSPDEIKNLYRRCRNGYEKLQVFRLLELDQD
                     HPVIRKFVNETYHIENEFICQLDPSRFDLIPEYVIMECDKLIALPPAANQSSVARIA"
     gene            complement(468..884)
                     /locus_tag="ACHOSM_RS25520"
                     /old_locus_tag="pgaptmp_005104"
     CDS             complement(468..884)
                     /locus_tag="ACHOSM_RS25520"
                     /old_locus_tag="pgaptmp_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4561382.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRAAVDATTKIKVALRLAGTPIGPN
                     DTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK"
     gene            complement(881..1111)
                     /gene="vapB"
                     /locus_tag="ACHOSM_RS25525"
                     /old_locus_tag="pgaptmp_005105"
     CDS             complement(881..1111)
                     /gene="vapB"
                     /locus_tag="ACHOSM_RS25525"
                     /old_locus_tag="pgaptmp_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HGU4561383.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
BASE COUNT          382 a          430 c          413 g          427 t
ORIGIN      
        1 gtggacagtt ttgattaccc gcgacttcag gctctcgtca gcagcccaga tgaaataaaa
       61 aacctgtacc gtcgctgtcg taacggctat gaaaaactgc aggtattccg tttactggaa
      121 ctggatcagg accatccggt aatacgaaaa ttcgttaacg aaacgtacca cattgaaaat
      181 gagttcatct gtcaactaga tccgtccaga ttcgatctca tcccggagta cgtgattatg
      241 gaatgtgaca agctcatcgc cctgccaccg gcagcaaacc agagctcggt tgcccgtatc
      301 gcttgagcca ctgtttttgc cgccggaaga taggctttag ctccctgata gtctgatctt
      361 cgaagatacc ctacagttta ggaccgctta ctgtagatat ctgttaaaac agcccgtata
      421 cgggctgttt cttggtgccc ttatctcctg actttgaaaa gagtgtctta cttcacccag
      481 tcttccagca ccaggtcagg cacccgctca aattctcttg tattattcgt taccagtatc
      541 gccccggcgg cgatggcgtg cccggcaatc gccgtgtcgt tcgggccgat cggcgtcccg
      601 gccagccgca gtgccacttt aatcttcgtg gtggcgtcta ctgcggcgcg atcccagggc
      661 aggatggcat cgaggcgggc gcagaattca tcgaccagtt ggacgtgacg tggtgaggcc
      721 tttgggccgg tagccccgaa gcgcatttca gagtaggtga tggccgagac cactatacgg
      781 tggccgcgta gcaccgcctg ctccagacgc ttcaggacgg cttctggctg ttcacgcatg
      841 atgaacgagc agatacaggt atcaagcata taagtcttgt tcacaggtta aatcgccctt
      901 cgtcgctgac aacatcctcg cgctccgcca taaaatctgg atcggctttt tcgtactcta
      961 ggaatgatcc ccaggttggc cggacggggc gcaggatgat gctgtcccct tcccggacga
     1021 tctcgagctc gctcaccccc tcaaaatcca gatcacgagg cagacggatg gcgcggttgt
     1081 tgccattttt aaatatcgat acggttctca tgttctttct ctcccgagca tatggatatg
     1141 tgaaacatat ccggaatatt caaagcaccg ggtgtgtgca tatgtttagt ataggcagat
     1201 gtgtggcata tgtatagcgg tagcatatgt agatgccatg catatgttta gcatatctct
     1261 atttctttct tttttatcgg agatccgaaa aaaggttgtt caggatcttc cttctccccc
     1321 cacacaacgc cacctcccgt catcacaaca tgtggtgccg gattcatctg ctgatgacac
     1381 tatatgttgt gtcatctccc tgacctgtga tgcgtcgcgc aggggcggaa aacagcgata
     1441 tgattatttc ctcagcgtga tacacttccg aaaacccgtc aataacagaa aaatcggatc
     1501 tgccggaaat ggtcctccga taatttaaca gcgtggttat atggatgctt gttatcatgg
     1561 tgatgatgat aacggcatga tgttatcacg cggcgtgacg gtaagagcag tgatgatgga
     1621 tgacgttatc gcatgaccgt ccctgcccgg aa
//
LOCUS       DAZKHC020000303         1629 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZKHC020000303 DAZKHC020000000
VERSION     DAZKHC020000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.305"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(267..494)
                     /locus_tag="ACHOSM_RS25530"
                     /old_locus_tag="pgaptmp_005106"
     CDS             complement(267..494)
                     /locus_tag="ACHOSM_RS25530"
                     /old_locus_tag="pgaptmp_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4561384.1"
                     /translation="MSSKNQPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
     gene            536..901
                     /locus_tag="ACHOSM_RS25535"
                     /old_locus_tag="pgaptmp_005107"
     CDS             536..901
                     /locus_tag="ACHOSM_RS25535"
                     /old_locus_tag="pgaptmp_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4561385.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGHGRADDIIGL"
     gene            1190..>1629
                     /locus_tag="ACHOSM_RS25540"
                     /old_locus_tag="pgaptmp_005108"
     CDS             1190..>1629
                     /locus_tag="ACHOSM_RS25540"
                     /old_locus_tag="pgaptmp_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4561386.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNPEMPPSAVMS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTH"
BASE COUNT          398 a          364 c          452 g          415 t
ORIGIN      
        1 aaaaacgata cacaccgggg tttaccgcgt aaacggagcg cggcgttctc ccctcatggt
       61 cgcccgtccg gtgcgatggt ggaagaaact ggaatctgtt caataaaaaa actgccgtgt
      121 tggagtcaca gcagtaatgt actgattggg tagaagatta ttattgttat gctttatttt
      181 tattctatat ggctgattat ttcaattcgg aattaataca gctaatgtct gtgagttttt
      241 ataaattcag caatataaag aaatagttat atgaacagcc atcgcagagc atactgtgta
      301 tcattctttt ttatagtcaa ctgacgggca tattttatgt ctgctgccag ctcccggcgg
      361 caagattcaa tgacccacgc agaaaaattt tctgaacctt tctggtcaag agcgatgtta
      421 atttgttcaa tcatctggtt tggaaatcgg atgttgcggg ttgttgttct gcggggctgg
      481 tttttcgatg acattttctt tcctctggtg acaagctata tggcgaggat tttacatggc
      541 tgtgcttcgt acgttaccgg gcagaatcaa aactctgaac acccggcggg tgaatattct
      601 gaagggtgaa cagcgtcgtg tcagtggcag tgcacgtgtt tccctcaagc gtcatatctg
      661 gctcagggat gccgggcagt gctgtctctg tggtcgtgtg gttgacctct gtgacagtga
      721 actcgatcac cgaattgcac ttcagttcgg tggtggtaat gaggagacga atctctggac
      781 gctctgtacc gaatgccatc gacaaaagtc tgctcgtgaa gcggcgggtg gtatgccgga
      841 cccgacgctg ccggaggtgt ccggaggtca tggcagggca gacgatatca tcggactgtg
      901 acccgccccg ggggggatca tccggcgaaa aaaacgatcg ctccggacac cgccccccct
      961 ctcacgcaga gaaaaaattc ctgtttcagg gcagttaaca tgttaactgg ctgcccgggc
     1021 attttttcgg tttttatctt tattattcag tttgttgtgc ggaaaaaatg ttaactggct
     1081 ttttcagcaa atgttaacca ggcagcagtt aacatttgcg gcatgagacg ccgggaaaaa
     1141 tgggctgaac catacccggc tgagtgcgtt atggacccgg gaggaggctg tgctgacaac
     1201 gcaaaaacga aaatttgcgc tggcgctcat gtccgggaaa aacaaaacag cgtcagccat
     1261 tgccgctggt tattcggcga agaccgccag ggttaaaggc tcgcagctgg caaaagatcc
     1321 ggaggtgctt gcgtttatag cccgtaaaca atgcgagacg gtggaggtgg atgaggttcc
     1381 tgtttaccgg cagaaaaaat cagagcagga ggataaaccc cgtcgccgtg aggcggctgc
     1441 aataccacag ccggacgaaa acaatccgga gatgccaccg tccgcggtga tgtctcctgg
     1501 tattgaatat atggaggatg gtcttcccga tccggtgaaa gcgatggggc gtcttctggt
     1561 ggagaacatt aataccgacc ccaggctggc gctggatgcg gcttataagc tggcgcagtt
     1621 cacgcacca
//
LOCUS       DAZKHC020000304         1625 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZKHC020000304 DAZKHC020000000
VERSION     DAZKHC020000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.306"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            89..>1625
                     /locus_tag="ACHOSM_RS25545"
                     /old_locus_tag="pgaptmp_005109"
     CDS             89..>1625
                     /locus_tag="ACHOSM_RS25545"
                     /old_locus_tag="pgaptmp_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HGU4561387.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALLEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAASCCPECGGSLSYLGEDAAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLISKYAEHTPLYRQSEMYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNA
                     GSTLAPAVLFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVA
                     LPTE"
BASE COUNT          366 a          466 c          493 g          300 t
ORIGIN      
        1 gcagcctaaa agactgctta cgtccctgac tatgttgtaa gcctctttat cctggtcgac
       61 gctgaatgag cctggtaata tacccggtat gagcagctca cttcctgacg atatcaatgc
      121 actgaaacgt ctccttgccg aacaggaggc gctgaaccgt gccctgctgg aaaagctgaa
      181 cgagcgtgaa cgcgaaatag accatctgca ggcacagctg gataagctgc gccggatgaa
      241 cttcggcagc cgctccgaaa aagtctcccg tcgtatcgca cagatggaag ctgacctgaa
      301 ggcacttcag aaagaaagtg atacccttac cggtcgggtt gacgacccgg ccgtgcagcg
      361 cccgctgcgt caaacccgca cccgcaaacc gttccccgaa tcactccccc gcgatgaaaa
      421 acggctgctg ccggcagcat catgctgccc ggaatgtgga ggctcactga gctatctggg
      481 tgaggatgcc gccgaacagc tggagttgat gcgcagcgcc ttccgggtta tccggactgt
      541 acgtgaaaag catgcctgta ctcagtgcga tgccatcgtg caggcccccg cgccttcacg
      601 gcccatcgag cggggtatcg caggaccggg gctgctggcc cgcgtgctga tctcaaagta
      661 tgcagagcac accccgctgt accgccagtc tgaaatgtac ggccgccagg gcgtggagct
      721 gagtcgttca ctgctgtcgg gctgggtgga tgcatgctgc cggctactgt caccgctgga
      781 agaagcgctt caggactatg tgctgactga cggtaagctc catgctgatg acacgcctgt
      841 cccggtgctg ttgccaggca ataagaaaac gaagaccggg cggttatgga cctacgttcg
      901 tgacgaccgt aacgccgggt caacgctggc gccggcggtg ttgttcgctt acagcccgga
      961 cagaaaaggc atccatccgc agacccatct tgcggggttc agtggtgtac tgcaggcgga
     1021 tgcatacgcc gggttcaacg agctgtaccg ggatggccgg ataacggaag ccgcctgttg
     1081 ggctcacgcc cgccgtaaaa tccacgatgt gcacgttcgc accccgtcag ccctgacgga
     1141 ggaagcgctg aaacggatcg gcgaactgta cgccatcgag gcagagataa ggggaatgac
     1201 ggcggagcag cgccttgccg aacgtcagtt gaaaacgaaa ccgctgctga aatccctgga
     1261 aagctggctg cgtgaaaaga tgaaaaccct gtcgcgacac tcagaactgg cgaaagcgtt
     1321 cgcatacgcc ctgaaccagt ggccggcgct gacgtactat gcagatgatg gctgggctga
     1381 ggcggacaat aacatcgctg aaaatgcgtt gcggatggtc agtctgggcc gcaaaaacta
     1441 cctgttcttc ggttcggatc atggaggaga gcggggagcg ctgctgtaca gcctgatcgg
     1501 gacgtgcaaa ctgaacggag tggagccaga aagctacctc cgctatgtcc ttgacgtcat
     1561 agccgactgg ccgataaacc gggtcggcga actgctcccc tggcgcgtag cactgccgac
     1621 tgaat
//
LOCUS       DAZKHC020000305         1599 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZKHC020000305 DAZKHC020000000
VERSION     DAZKHC020000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.307"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..81)
                     /locus_tag="ACHOSM_RS25550"
                     /old_locus_tag="pgaptmp_005110"
     CDS             complement(<1..81)
                     /locus_tag="ACHOSM_RS25550"
                     /old_locus_tag="pgaptmp_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561388.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHHA"
     gene            complement(134..538)
                     /gene="ycgX"
                     /locus_tag="ACHOSM_RS25555"
                     /old_locus_tag="pgaptmp_005111"
     CDS             complement(134..538)
                     /gene="ycgX"
                     /locus_tag="ACHOSM_RS25555"
                     /old_locus_tag="pgaptmp_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HGU4561389.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(759..1490)
                     /gene="bluR"
                     /locus_tag="ACHOSM_RS25560"
                     /old_locus_tag="pgaptmp_005112"
                     /pseudo
     CDS             complement(759..1490)
                     /gene="bluR"
                     /locus_tag="ACHOSM_RS25560"
                     /old_locus_tag="pgaptmp_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
BASE COUNT          434 a          346 c          338 g          481 t
ORIGIN      
        1 ggcatggtga ttatttatgg gtagcaataa aaggacagta tttatcatcc atagggatag
       61 tctctgtact tttattccca ttatgctaat gccttactga attatgaagc atttcttaag
      121 catccaactt tagctagatt aatggtttat tattttctac atcttcaata tataaaagcg
      181 tattatcaat ggcgtagtaa ctgcgtttgt tatgattaac atcagtaacc caacggaaaa
      241 cgcccgcgcc tgctagtgtt gaacagtatt cccgaaatgt agattttccg caaatatgaa
      301 gcaatgcggc ctcttttatt ttagcagggt tcttggtcgt actaactttt aacaggttcc
      361 tggttcctct taataacaaa accgtgtcat cgtgagtaat aattctgatg ttatccgtag
      421 ccagataata aatgtaatgt gcaatacggt gatgttttaa ttctgaataa aaccaggaga
      481 agttttgctc ttttctcact tgctcaaaca tcttttgaaa aacaacgacc tgatccatca
      541 ggataataac ctcttgttag ttgtgagact gcgtagtgtg cacgatcggt tttaccactt
      601 caatctggtc tgtcctttgg ctgtgatatg tacagagtgt gatagaggga atatctgaat
      661 tctcccggtg agcattttgc aacggaccag ctccggtaca aacgctgttg tgggttcaga
      721 ttataacatt ctgtctaagg ggcgggataa aggtgaaatt agggggcatg aaagatgact
      781 ttataacctt gctcacccca atgttgtaaa agttcgtttt gccttctcgt tggtgccatg
      841 cctgtccaga caatcaatgt ttgcgtcggg aacagttccg ggcgtggcga ttcaatggga
      901 tcagcaagaa cagaaatgtg ccatcctgac aatgataaac gccatgcttc cagccacaat
      961 cgtgctctct cttcaacatt ccacgccatc agaatggctt ctttaccggg cttacggcgc
     1021 atttcgaaaa gcgaggctgc tgtgtattca attaatgcgc cgtcaaacat actgctcata
     1081 atgcgggagg tgttgtgatc aagcacgaga cgctggcgaa caggaaggta aacatgatta
     1141 atcaattgat ctactgggta ctctcgaccc agtgaaataa ttctcgcgcg tagtttggca
     1201 ggattagcca tacgaagaat tgacatcatc tcttcttgca agcggctcca gtcatcttcc
     1261 gtatcctggc tggtggtttc cagtaatgct ttaactttgc ctacagggac gccattactt
     1321 atccaacgct tgatctcttc gatacgttgt atgtcttctt catcaaagag tcggtgtccg
     1381 ccttcactgc gctgtggttt taacaaaccg tagcggcgtt gctaggcccg gagagtgaca
     1441 ggattaatcc cgcaacgttc agcaacatca ccaatgctgt aataagccac aattcctcct
     1501 tgcggtcata aatctccgcc gcctgtacac gacccaataa tactttgtac aatatacgct
     1561 aaaattgtac aaagtataaa taagatttca gctaaattg
//
LOCUS       DAZKHC020000306         1577 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZKHC020000306 DAZKHC020000000
VERSION     DAZKHC020000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.308"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..173)
                     /locus_tag="ACHOSM_RS25565"
                     /old_locus_tag="pgaptmp_005113"
     CDS             complement(<1..173)
                     /locus_tag="ACHOSM_RS25565"
                     /old_locus_tag="pgaptmp_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGU4561390.1"
                     /translation="MANTVKISSCELINADCLEFIQTLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYL"
     gene            complement(324..521)
                     /locus_tag="ACHOSM_RS25570"
                     /old_locus_tag="pgaptmp_005114"
     CDS             complement(324..521)
                     /locus_tag="ACHOSM_RS25570"
                     /old_locus_tag="pgaptmp_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4561391.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRECCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(737..1117)
                     /locus_tag="ACHOSM_RS25575"
                     /old_locus_tag="pgaptmp_005115"
     CDS             complement(737..1117)
                     /locus_tag="ACHOSM_RS25575"
                     /old_locus_tag="pgaptmp_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4561392.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSTTKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            complement(1136..>1577)
                     /locus_tag="ACHOSM_RS25580"
                     /old_locus_tag="pgaptmp_005116"
     CDS             complement(1136..>1577)
                     /locus_tag="ACHOSM_RS25580"
                     /old_locus_tag="pgaptmp_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4561393.1"
                     /translation="IIPGEQTATSILKQRTKNIALPPHTHQQQNPPQEKTVVSIAVDP
                     ESPESFMKRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKAHDLF
                     VLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
BASE COUNT          386 a          419 c          341 g          431 t
ORIGIN      
        1 ttcaggtaat catcgtcgcc cttccactgg ttatcccagc cctcaggctt cactttaaag
       61 tatggcgggt ctgtgactat cagatcgaca gagttttccg gtaaggtctg gataaattcc
      121 aggcaatcag cgttgattaa ctcacaactg gatattttta cagtattagc catagatcaa
      181 taagcacttc tctgataggc tcattctgct tttgcgcaaa gcagatgggc ctgaggtttg
      241 cttgtgaccc caacgcatga gcagatggct ggtgagtgcc ctaacaccca ccagccgccc
      301 atttaccaca aataaaaaag ccttcaggac tgaaggcgtc tgtaacaacc gaactgatag
      361 tctgccagac ccgccataac aagctgggtc agtattaact gacagcattc gcgtgaaagg
      421 taagtattct gtgcaatctc cccgactgtc gccggttcgg tgacgcttaa ttcattaaac
      481 accactctgg cggtttcggt catatcctgc tgtttcagca tgtctttttc ccttttccgg
      541 ttaacgtgac acaccaataa ctcttgtcaa aaaagccagc aagctgaaag accggtattc
      601 accgccacca gcgcgtttac tgtacaggac cgatttcagc cataaaaaaa cccgctcgcg
      661 gcgggtttaa gctgtgtggc aaagtaacca ctcttaacat actgacatac tttttgcgga
      721 ccgcactaat cattttttac ttttttagca gccagtcgtc catctccagt cttaccccca
      781 gcacagacaa acatccgtca ataaaccctt cggctatctg catctcaatt cgtattgctt
      841 tttcgctttt ctttctcgtc ctggctatct gtctttttga tattcgcaac aaataatgag
      901 caatgagaag cgaatactcc tcaggttttt tctgcttcag acgtgcaaga cagttttcaa
      961 tgataagtcc gtcatcatcg cagcaggctg gacgtggttt agtggtagat ggtaaaaggc
     1021 ctttgaatcc ggcagcgatc ggagaatagt ccaccccggt gttaccactt gccgcccatg
     1081 ccccccagcg ttcaagaacc atctgaatat cacgcatcaa ctttctccac aaaaatcagg
     1141 acagcacacc aatcgccagt gcgcgatcga taaaacgaaa tatcagctcc agttgggaac
     1201 catacttctc ttcaaatgcc acggtatccg catgcagttc gtcatggtgt tttctgcaca
     1261 aaggcaacac aaaaaggtca tgcgcttttg tacccattcc accctgacca tgaccaatca
     1321 gatgatgagg atcgtccgct ggcttaccac aacacgcaca cggctgtgtc tttacccagc
     1381 gtgtgtattt ctcatttacc cagcggcgac gtttaggtcg tttcatgaag gattccggag
     1441 actccggatc aacggcaatg ctgaccaccg tcttttcctg tggtgggttc tgttgctggt
     1501 gggtgtgagg cggtagcgca atattttttg tgcgctgctt cagtatgctg gtggcggtct
     1561 gttctcccgg tatgatg
//
LOCUS       DAZKHC020000307         1556 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZKHC020000307 DAZKHC020000000
VERSION     DAZKHC020000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.309"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            309..1520
                     /gene="bluF"
                     /locus_tag="ACHOSM_RS25585"
                     /old_locus_tag="pgaptmp_005117"
                     /pseudo
     CDS             309..1520
                     /gene="bluF"
                     /locus_tag="ACHOSM_RS25585"
                     /old_locus_tag="pgaptmp_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
BASE COUNT          462 a          314 c          320 g          460 t
ORIGIN      
        1 gcctctgtta aaaatgttaa tcaatgtgaa gtgaggtaac aatagcaaac ttgtacgaaa
       61 tatgtgtaca gaatgataaa ttaatttttt gcaattagtg ctaatgcata taaaacaatg
      121 tgttatgata aaacatattg catcatttaa ctgacaatct atacaagatt tttcgccaac
      181 tagtgttcaa ctttttccat tttttatcaa tgtaaagaaa cggttatgtt ttggtattaa
      241 aagaaagaca caagaagata tgtctgttac cgtcttactc tcgcctcacc cattaccctg
      301 gattgttaat gcttaccacc cttatttatc gtagccatat acgtgacgac gaacctgtca
      361 aaaaaatcga agaaatggtt tcgatagcaa atcgcaggaa catgcagtct gacgtaacag
      421 ggatcttact gtttaatggt tctcattttt tccagcttct ggaaggtccg gaagaacagg
      481 ttaaaatgat atatcgggct atatgctagg atccacggca ctataatatt gttgagctgc
      541 tgtgcgatta cgcgcctgct cgccgttttg gcaaagcggg aatggaatta tttgatttgc
      601 gcctgcacga gcgagatgac gttttacagg ccgtattcga caaaggcaca tcaaaatttc
      661 agctaactta tgatgacaga gcgctacaat tttttcgtac ttttgtcctt gcaaccgaac
      721 aatcaaccta tttcgagatc cctgccgaag actcctggct ttttatcgct gacggatctg
      781 ataaagaact tgattcctgt accctttcac caactataaa cgaccacttt gcctttcatc
      841 ctattgtcga tcccttatcg cggcggataa tcgcttttga agccattgtg caaaaaaatg
      901 aagatagccc atcagccata gcggttgggc agcgtaaaga cggggaaatc tacacagcgg
      961 atctcaaaag taaggcgctt gcattcgcga tggcacacgc acttgagctc ggtgataaaa
     1021 tgatttcaat caatctatta cctatgaccc tggttaacga acctgacgca gtctcttttt
     1081 tacttgatga aataaaggcc aatgctctgg tgcctgaaca aatcatcgtt gaatttactg
     1141 aaagtgaagt tatatctcgg tttgatgagt ttgcagaagc gattaaatcg cttaaggctg
     1201 ccggtatcag tgtagcaatt gatcattttg gcgcaggttt tgccggtttg ttactcctgt
     1261 cacgcttcca gcctgacaga attaaaatca gtcaggaatt gattaccaat gttcataaaa
     1321 gcgggccacg gcaggcaatt attcaagcga tcataaaatg ctgtacatca cttgaaattc
     1381 aagtcagtgc tatgggcgtg gcaacaccag aagagtggat gtggcttgaa tccgcaggaa
     1441 ttgagatgtt tcagggagat ctgtttgcta aagctaaatt gaatggtatc ccttccatag
     1501 cgtggccaga gaaaaaataa ttttcagcac attctttcac atgatttcag taaatc
//
LOCUS       DAZKHC020000308         1552 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZKHC020000308 DAZKHC020000000
VERSION     DAZKHC020000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.310"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..147
                     /locus_tag="ACHOSM_RS25590"
                     /old_locus_tag="pgaptmp_005118"
     CDS             <1..147
                     /locus_tag="ACHOSM_RS25590"
                     /old_locus_tag="pgaptmp_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4561394.1"
                     /translation="VKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTES
                     QHEE"
     gene            92..>1552
                     /locus_tag="ACHOSM_RS25595"
                     /old_locus_tag="pgaptmp_005119"
     CDS             92..>1552
                     /locus_tag="ACHOSM_RS25595"
                     /old_locus_tag="pgaptmp_005119"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGU4561395.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLV"
BASE COUNT          390 a          374 c          453 g          335 t
ORIGIN      
        1 gtcaaacgtc gccgcaaggc tgaaattgat gaaaacagca gactggggct ggtctttgat
       61 actgaccccg tcaacgacaa aggaggcaac agtgccggaa ctgaacgaca gtatcagcgc
      121 gacaccgaaa gccagcatga agaataaatc ctggttcagg atgcaggctg gggggccggg
      181 tgacgcggat atttatattt atgacgagat tggtttctgg ggagttaccg cgaagcagtt
      241 tgtcagcgaa ctgaatgcac tgggtgatat cacccacatt aatctccata tcaattcacc
      301 gggtggcgat gtctttgaag gcatcgccat ttttaatgcc ctgaaaaatc agggggcgac
      361 cattaccgtg tatgtggatg gcgttgccgc ctcgatggca tctgtgattg cgatggccgg
      421 tgatacggtc attatgccgg aaaatgcctt catgatgatc cataagccat ggggattcag
      481 tggcggggat gctgaggata tgcgcagtta tgccgatttg ctggataaag tcgaatcggt
      541 actgttgcca gcctatgcgc agaaaaccgg aaaaaccacc gatgaaattg ccgccatgct
      601 ggcggatgaa acctggatgt ccggtgccga atgtctggca cacggatttg ctgaccaggt
      661 gacacccgct gttgaggcaa tggcatgtat tcagtcaaaa cgtacagagg aatttaaaaa
      721 gatgccggaa tccatccgaa acatgattac tccgccacgc aacagtgccc cgcgtgatac
      781 cacagtgaca atccctgcac cggcggtaac agaaccatca ccggtaccgg cagtgtctga
      841 tgaggcgacc attcgcgccc gcgttatggc agaacagaaa gcccgcatgt caggcattaa
      901 cgatctgttt gccatgttcg gtggtcgcta tcagacgctt caggcacagt gcgtggctga
      961 tcctgactgt tcgctggaaa tggcccgtga acgactgctg aatgaaatgg gcaaggagtc
     1021 ctcgccgacc aacaaaaata caccggccca tatttatgcc ggaaacggca attttgtggg
     1081 ggacgggatc cgccaggcga tgctggcccg tgccggattt gaaaatgtcg agaaggataa
     1141 cgcctataac gggatgaccc tgcgtgaatg ggctcgcatg tcactgacgg agcgcggtat
     1201 tggggtggcc agttataacc ccatgcagat ggtcgggctg gcgctgacgc acagcacctc
     1261 tgattttggc aatattctgc tggatgtgtc gaacaagggg ctgatccagg gctgggagga
     1321 atcagaagaa accttccaga agtggacccg taagggacgc ctgtcagact tcaaaacagc
     1381 gtatcgcgtg gggatgggcg gttttggttc tctgcgccag gttcgtgagg gggcggagta
     1441 taaatacatc accacctcag atcgcaagga gaccattgca ctggccactt acggggagat
     1501 tttctccatc acccgccagg ccattatcaa tgatgatctg aatatgctgg tg
//
LOCUS       DAZKHC020000309         1532 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZKHC020000309 DAZKHC020000000
VERSION     DAZKHC020000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.311"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..668
                     /locus_tag="ACHOSM_RS25600"
                     /old_locus_tag="pgaptmp_005120"
     CDS             <1..668
                     /locus_tag="ACHOSM_RS25600"
                     /old_locus_tag="pgaptmp_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4561396.1"
                     /translation="TPEGERYQPCVNISAEPEAYFRIAPEDWLQAEMQGEIVALVHSH
                     PGGLPWLSEADRRLQIKSALPWWLVCRGEIHKFRCVPHLTGRRFEHGVTDCYTLFRDA
                     YHLAGITLPDFVREDDWWRNGQNLYLDNMAATGFYRISLPSAQPGDILLCCFGASVAN
                     HAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSAWRHRHWHVSAFTGIYNDLAAAS
                     ACM"
     gene            665..1246
                     /locus_tag="ACHOSM_RS25605"
                     /old_locus_tag="pgaptmp_005121"
     CDS             665..1246
                     /locus_tag="ACHOSM_RS25605"
                     /old_locus_tag="pgaptmp_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4561397.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKVPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
BASE COUNT          337 a          386 c          491 g          318 t
ORIGIN      
        1 gcaccccgga gggcgaacgg taccagccct gcgtgaatat ctccgcagag ccggaggcgt
       61 attttcgtat tgcgccggaa gactggctgc aggcagagat gcagggggag attgtggcgc
      121 tggttcacag ccaccctggt ggtctgccct ggctgagcga ggcggaccgg cggctgcaga
      181 taaagagtgc cctgccctgg tggctggtct gccgggggga aattcacaaa ttccgctgtg
      241 tgccgcacct gaccgggcgt cgttttgagc acggggtgac ggactgttac accctgttcc
      301 gggatgcata ccatctggcg gggataacgc tgccggattt tgtgcgtgag gatgactggt
      361 ggcgcaacgg tcagaacctt tacctggaca atatggcggc gactggtttt tacaggattt
      421 ccctgccttc cgcacagcct ggcgatatcc tgctgtgctg ctttggcgca tcggtggcca
      481 atcatgccgc catatactgc ggcaacggtg agctgcttca ccatctgcct gaacaactga
      541 gtaaacggga gaggtattcc gaaaaatggc aacgacgaac gcattcagcc tggcgtcacc
      601 gccactggca cgtatctgcc ttcacgggga tttacaacga tttggccgcc gcctcagcct
      661 gtatgtgaac acggcagcgg aagccatccg tgccctgtcg atgcagatgc cgggattccg
      721 ccgtcagatg aacgaaggct ggtaccagat acgtattcgc ggtgaggaca cggcaccgga
      781 ggcggtgtac gcccgtcttc acgaacagct gggtgaggga acggtcatcc acattgtgcc
      841 gcgactggcc ggggccggaa aaggtggact gcagattgtg ctgggggcgg cagccatcgt
      901 gggctctttc ttcactgccg gggcaacgat ggcgttatgg ggttcagccc tggcagccgg
      961 tggtttttct gccaccacga tgctgttttc actgggggcc agcatgatac tgggtggtgt
     1021 ggctcagatg ctggccccga aggctaaagt accggagtac aaaagcacgg ataacggcag
     1081 acagaacacg tacttttcct cgctggataa catgattgca caggggaacc cgatgccggt
     1141 gccttacggt gaaatgctgg ttggctcacg gcgaatctcc caggacatca gtacccgtga
     1201 tgaaggcggt gacgggaagg tggtggttat cgggcggcag gcataaaagc gaaaaaatcc
     1261 cgcagtgacc gaaggctgcg ggaacagaaa atgaagatta accacaggga gttttgtttt
     1321 tattggcccg aaaaaactgt aacgcccggg aatgatatct gccacggggg cgtacagaaa
     1381 atgtgaagat attcagaatt tttattcagt catgatacag gcatcctccg ggatgcctgt
     1441 tgtttttgtg cgtaacagtt atcacagtaa agggtgagac aatgggcaaa ggtggcggca
     1501 aggcgcacac gccggttgag gcaaaggaca at
//
LOCUS       DAZKHC020000310         1525 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZKHC020000310 DAZKHC020000000
VERSION     DAZKHC020000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.312"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..873
                     /gene="wzx"
                     /locus_tag="ACHOSM_RS25610"
                     /old_locus_tag="pgaptmp_005122"
     CDS             <1..873
                     /gene="wzx"
                     /locus_tag="ACHOSM_RS25610"
                     /old_locus_tag="pgaptmp_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024226091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O177 family O-antigen flippase"
                     /protein_id="HGU4561398.1"
                     /translation="SIPLLLLNQLWVSVFEGEEKFGLINIQKTISNTCIVALPAVSIL
                     IESSLTYAVSGLVVGRVISLILSFLFLRKEIVASGIRFHTIVMKRLIMFGSWMTFSNI
                     ISPMMVYFDRFIISNILGAKNVGFYTAPAEIISRMSIIPTSVSRALFPRLSNMSDFKK
                     FKCELLFSYAIMISICVPIVLFLLLFSGGIMYYWLGPQYYLKSSSVFSVLLIGFFFNS
                     LAQLPFSAIQSLGNSKITALLHGFEIIPYLIILYILTSHFGIVGTAYAWTLRVMFDFI
                     ALYFLSSWLIKKKH"
     gene            886..1452
                     /gene="rfbC"
                     /locus_tag="ACHOSM_RS25615"
                     /old_locus_tag="pgaptmp_005123"
     CDS             886..1452
                     /gene="rfbC"
                     /locus_tag="ACHOSM_RS25615"
                     /old_locus_tag="pgaptmp_005123"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HGU4561399.1"
                     /translation="MKIANTKLDGLLVIEPTVYEDERGFFYESYNEKKFMETIGASIK
                     FVQDNHSKSSKGVLRGLHFQKNPYEQGKLVRCIRGEVYDVAVDIRKDSPTFGQWFGVN
                     LSDENKKQLWIPEGFAHGFITLSDVAEFVYKTTNYYAPASEYCLKWDDKDLNIQWPLK
                     NALIVSSKDQKGKAFADFIIESNEEQRW"
BASE COUNT          484 a          207 c          288 g          546 t
ORIGIN      
        1 tctataccac tacttttatt aaatcaattg tgggtttcgg tgtttgaagg ggaagaaaaa
       61 tttgggttga tcaatattca gaaaaccata tcgaacactt gcattgttgc gttgcctgct
      121 gtaagtatat tgatagaaag ttccttgacc tatgcagtat cagggcttgt agtcgggcga
      181 gtaatatcct taattttgtc ttttttgttt ttacgtaaag agatagttgc atcaggtatt
      241 agatttcaca ccattgtcat gaaaagattg ataatgtttg gtagttggat gacgtttagc
      301 aatattataa gcccaatgat ggtgtatttt gatcgattta taatttcaaa tatattaggt
      361 gcaaagaatg ttggattcta tactgcacca gcagaaataa tatcaaggat gagtattatt
      421 ccaacatcag tgtctagagc tctatttccc cgacttagta atatgagtga ttttaaaaaa
      481 tttaaatgcg agcttttatt ttcgtatgcg ataatgatat cgatttgcgt gcctattgtt
      541 ttatttcttc ttctattttc gggtgggata atgtattatt ggttggggcc gcagtattat
      601 ttaaaatcct caagtgtttt ttctgtatta ttgattggtt ttttctttaa ttctcttgcg
      661 caactgcctt tttctgcaat tcaatcccta ggtaattcta aaataactgc attactccat
      721 ggttttgaaa ttattccata tcttataatc ttgtatattc taactagcca tttcgggata
      781 gtaggaacgg catatgcatg gacattgaga gttatgttcg attttattgc actatacttt
      841 ctttcatcct ggttaataaa aaagaaacac taaaaaggta attttatgaa aatagcaaat
      901 acaaaacttg atggtttact ggttattgaa cctactgttt atgaagatga gagaggattt
      961 ttttatgaaa gttataatga aaaaaaattt atggagacaa taggagcgtc aataaaattt
     1021 gtccaagata accattcaaa atcgtctaaa ggggtccttc gcggcctgca ttttcaaaaa
     1081 aatccttatg aacaaggtaa actagttcga tgtatccgag gagaagttta tgacgtagct
     1141 gttgatataa gaaaggactc tccaacattt ggacaatggt ttggcgtgaa tttatctgat
     1201 gaaaataaaa aacaattatg gataccagaa ggatttgctc atgggtttat aacattaagt
     1261 gatgttgcag aattcgtata taagacgaca aactattatg ctcctgctag tgaatattgt
     1321 ctaaaatggg atgataaaga tttaaatatt caatggcctt taaaaaatgc tttaatagta
     1381 tcaagtaaag atcagaaagg gaaagctttc gctgatttca taattgaaag caatgaagaa
     1441 caaaggtggt aatgtgagct taattcagag agtatcttct cttttgatag cgttaaccat
     1501 ttatctattt ttatttgatg ttatt
//
LOCUS       DAZKHC020000311         1513 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZKHC020000311 DAZKHC020000000
VERSION     DAZKHC020000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.313"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..180
                     /locus_tag="ACHOSM_RS25620"
                     /old_locus_tag="pgaptmp_005124"
                     /pseudo
     CDS             1..180
                     /locus_tag="ACHOSM_RS25620"
                     /old_locus_tag="pgaptmp_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            478..825
                     /locus_tag="ACHOSM_RS25625"
                     /old_locus_tag="pgaptmp_005125"
     CDS             478..825
                     /locus_tag="ACHOSM_RS25625"
                     /old_locus_tag="pgaptmp_005125"
                     /inference="COORDINATES: protein motif:HMM:NF014559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HGU4561400.1"
                     /translation="MFTQHIEKQRNVNTLNQNGINNSVNNANVHENELTYQFQRWGQN
                     HTVRILESSEGIRLKPSDTLVSNRLHEAQHNDVTAQRWVLTEQDERQGQRHQPYEEQE
                     NESKFENDQKDES"
     gene            785..1513
                     /locus_tag="ACHOSM_RS25630"
                     /old_locus_tag="pgaptmp_005126"
                     /pseudo
     CDS             785..1513
                     /locus_tag="ACHOSM_RS25630"
                     /old_locus_tag="pgaptmp_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT          561 a          244 c          302 g          406 t
ORIGIN      
        1 cccgataatt ctgcttcgaa tttagacagt ttcttaaaga aaaacaaaaa aaataaagaa
       61 atgagtgaga ttgtcgctgg aaccattctc cagttattac gtcagactca ggatagccaa
      121 ttttctacat tggggaaaaa gaatgggcaa atgaagtcta ttgacaacaa atcatctctc
      181 caggttgaca ggaatccctc tctttctttg caaacaaatg gaacagaaca attttctaca
      241 aagaaaattc tcaatatgac ttctcgagat cagggtatta gttctgaact atctaataaa
      301 acgatacaaa ttaatgaaaa aatccataat ggtatccata cagaatacat cacagatcaa
      361 aaaaacagta ataataaaga tcgtgaaaaa aaataccgcg atggagacaa aataaatgga
      421 ccgcaggccc attcattaga cagtactaac gaaagaaggt ttgctgataa cagaaccatg
      481 tttactcaac atatcgaaaa gcaaaggaat gttaatacgc tcaatcagaa tggtataaat
      541 aattctgtta ataatgccaa tgtgcacgaa aatgaattga catatcagtt tcagcgttgg
      601 ggacaaaatc atacagtccg gattctggaa agtagtgaag ggatacgact aaaaccatca
      661 gacacacttg tctccaacag actccatgaa gcccaacaca atgatgttac agcacaacgt
      721 tgggtattga ctgagcaaga tgaacgacaa ggacaacgac atcagcctta tgaggagcaa
      781 gaaaatgaaa gcaaattcga aaacgatcag aaggatgaaa gttaatgttc ggtctgagga
      841 aagtaaatcg aaacactcat acgtttgaaa ccacttttca aaattggaaa gaaaatggcg
      901 aagatgttgc attattaatg cccgaattta gtgctaaatg gttaccgata gcagaagaga
      961 gtggcagctg gtctggatgg gtattgcttg gagaaatatt tcccttaatt tcatctgagc
     1021 tcgctgggat ggctttaatg ccagaaacgg aaaggttaat aggggaatgg ctcagtttat
     1081 ccagttcccc gttaaatcta aagcatcctg aactaaaata taatcgtttg cacgtaggta
     1141 aagtatttga tggagtattg agccctgctc agccactaat aagaatatga acaggggaat
     1201 tgaatctttg gttagataaa gtcacagtct gccaatacgg aaacgctcca gcgttagaca
     1261 aaaaatcatt atattggtcc attcattttg taattggatt tagtaaaaca tgttacagaa
     1321 gtcttgttga tattgaagtt ggtgatgttt tattaatctc aaataatttg gcttatgcgg
     1381 ttatttataa tacaaaaact tgtgatttaa tttatccaga ggagttaaaa atggctgatc
     1441 attttgagta cgaggaagat tttgaaacag atgattttga tatcaaaaaa acgagagtga
     1501 gatttatgat gaa
//
LOCUS       DAZKHC020000312         1490 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZKHC020000312 DAZKHC020000000
VERSION     DAZKHC020000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.314"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            169..1389
                     /gene="escD"
                     /locus_tag="ACHOSM_RS25635"
                     /old_locus_tag="pgaptmp_005127"
     CDS             169..1389
                     /gene="escD"
                     /locus_tag="ACHOSM_RS25635"
                     /old_locus_tag="pgaptmp_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HGU4561401.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADQSLPVGKVIDIAGCCFIIGDIDHTLP
                     LSDVPERFSAKNRRKKRLILASVIGAAIALSGAIGSYVLLSPKAEPPAFTRADVYQQL
                     KENKLHAITLVWHGKNVALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNNQIISAIN
                     DILTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     KINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNVKILF
                     QNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGIN
                     ISRPDEYIHIPLSY"
BASE COUNT          480 a          292 c          274 g          444 t
ORIGIN      
        1 tgattaaatt caataccatt agccattgga aactcacgta atcattaatt atgaatattc
       61 cattaaatag tactgtacaa cagcttcctt tctactctat tcttagttga tgtaagttca
      121 ccatattttt tctcattgtt caaccaccag gatgaataaa atttaaaaat gttatcctca
      181 tataaaataa aacttctcaa tggtgcgatg cgtaatcggg aattacaact tccgatggga
      241 aacctaacga taggcacaga agataacgat attgtttatt ttccccttga acaaggcctc
      301 aatcagtttt tacttgatat acgtgaggaa ggtgttttcc ttttatctcc ggttgaattt
      361 tggattgatg gacagcccac cccctatgaa gcagatcagt cattacctgt cggaaaggtc
      421 atcgatatag cgggatgttg cttcatcatt ggtgatattg accacacctt gccattgtct
      481 gatgtgccag aaaggttctc tgccaaaaat cgacgtaaaa agcgtcttat cctggctagc
      541 gtgattggtg ctgcgattgc gttgtctggc gccatcggca gttacgtttt gctttcccca
      601 aaagctgaac ccccagcgtt taccagagct gatgtatacc agcagctaaa agaaaacaaa
      661 cttcatgcaa tcactctggt atggcatggg aaaaatgtcg cgctttatgg gcgttgcgaa
      721 tcaaccactg atttaactcc attttttaat tacctgaaag aaaaaaatat tttttattac
      781 aacaatatca tttgcaataa ccaaatcatc tccgcgatta atgatatttt aacggaatac
      841 ggttataagg atatcatcat tacaaaaggt aataagcctg gattcttcct gttatcggga
      901 tacattcccc cttcaccaaa atggagcgaa gttgaaaatc tgttactgaa tacgccaggg
      961 gttgctggat gggaaataca taacaacagt aacaataaaa tcaatgaact ggcatcagag
     1021 ttcaaaaaga ataaattaat aaactatgtc aatattttca aaaagaatga tgtcatcatt
     1081 gttgcggggg aggtttctca acagaacgag agtaagatcc ttgccattat aaatgcgatg
     1141 aacaaaaata gtaacgtcaa gatactattt cagaacattc agccgtacat ttcagcagat
     1201 attttccccg gaaaaatact cagaatcagc ggcacgatga agaatccaac aattgctctt
     1261 gataacggta caagcctggg aattggtagc attctgaaag gaggctatgt catagacgcg
     1321 attgatccta aagatggtat caacatctcc cgcccagacg aatatataca tattccgttg
     1381 tcttattaac ttattattat cttccccctc ttcggagggt tttttattgt gcagatgttg
     1441 tagatatggt aaacaaggtt tctatcaaac aagatacacg aaaaacttat
//
LOCUS       DAZKHC020000313         1481 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZKHC020000313 DAZKHC020000000
VERSION     DAZKHC020000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.315"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            339..527
                     /gene="dicB"
                     /locus_tag="ACHOSM_RS25640"
                     /old_locus_tag="pgaptmp_005128"
     CDS             339..527
                     /gene="dicB"
                     /locus_tag="ACHOSM_RS25640"
                     /old_locus_tag="pgaptmp_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4561402.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            524..715
                     /locus_tag="ACHOSM_RS25645"
                     /old_locus_tag="pgaptmp_005129"
     CDS             524..715
                     /locus_tag="ACHOSM_RS25645"
                     /old_locus_tag="pgaptmp_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4561403.1"
                     /translation="MNTAFALVLTVFLVSGEAVDIAVSVHRTMQESMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            809..>1481
                     /locus_tag="ACHOSM_RS25650"
                     /old_locus_tag="pgaptmp_005130"
     CDS             809..>1481
                     /locus_tag="ACHOSM_RS25650"
                     /old_locus_tag="pgaptmp_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4561404.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIICEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVIDLARA
                     RTNQQH"
BASE COUNT          415 a          324 c          360 g          382 t
ORIGIN      
        1 ggcgcggcgg taagtatggc ggggttattc cttccccgtt gaggacaccg ggttgtcagg
       61 ttgaccatac gcttaagtga caaccccgct gcaacgccct ctgttatcaa ttttctggtg
      121 acgtttggcg gtatcagttt tactccgtga ctgctctgcc gcccttttta aagtgaattt
      181 tgtgatgcgg tgaatgcggc taagcgcacg cggaacagtt aaaaccaaaa acagtgttat
      241 ggttggattc tctgtatccg gcgttaattg ttaactggtt aacgtcacct ggaggcacca
      301 ggcaccgcat cacaaaactc attgttgagg gcgcgataat gaaaacgtta ttaccaaacg
      361 ttaatacgtc tgaaggttgt tttgaaattg gtgtcactat cagtaaccct gtatttactg
      421 aagatgccat taacaagaga aaacacgaac gggagctatt aaataaaata tgcattcttt
      481 caatgttggc ccgtttacgt ccgatacaaa aaggatgctg gcaatgaata cagcatttgc
      541 acttgttctg acagtttttc ttgtttccgg agaggcagtt gatattgcag tcagtgttca
      601 caggacaatg caggagagta tgactgcagc aaccgaacag aaaattcccg gtaactgtta
      661 cccggtcgat aaagttattc accaggataa tatcgaaatc ccggcaggtc tttaaaacag
      721 ttccgtaata aatatccggt ttcattctta tatgccagca atggcaggga tttgttcatc
      781 cttaaatctg tcatgaggtt aaaacaaaat gagtaaagtc tttatttgcg ccgccattcc
      841 tgacgaactg gcaacaaggg aagaaggcgc tgtggctgta gccacagcta ttgaagctgg
      901 cgacgaacgc cgtgctcgag caaaatttca ctggcaattc ctggaacatt atccggctgc
      961 tcaggactgc gcttataaat ttattatctg cgaggataaa cctggcatac cccgccctgc
     1021 cctcgattca tgggatgctg aatatatgca ggaaaaccgc tgggatgagg agtctgcttc
     1081 ctttgttccg gttgagactg aatcagatcc gatgaacgtc acttttgaca agctggcccc
     1141 tgaagtacag aacgctgtca tggttaagtt cgacacatgt gaaaacatca ccgttgatat
     1201 ggttattagc gcacaggaat tgttgcagga agacatggca acattcgacg gacatatcgt
     1261 tgaagcgttg atgaaaatgc cagaagttaa cgccatgtat ccggagctta agctgcatgc
     1321 catcgggtgg gttaagcata aatgtaagcc tggtgccaaa tggcccgaaa ttcaggcaga
     1381 gatgcgcatc tggaaaaaac gtcgcgaagg tgaacgcaag gaaaccggaa aatacacgtc
     1441 tgttattgat ctcgcccgcg ccagaaccaa tcaacagcac a
//
LOCUS       DAZKHC020000314         1457 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZKHC020000314 DAZKHC020000000
VERSION     DAZKHC020000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.316"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..459)
                     /locus_tag="ACHOSM_RS25655"
                     /old_locus_tag="pgaptmp_005131"
     CDS             complement(<1..459)
                     /locus_tag="ACHOSM_RS25655"
                     /old_locus_tag="pgaptmp_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU4561405.1"
                     /translation="MRIYRRKCKCCNEWFIPKYQNQYLCNEICGTKIALERRSKEREK
                     AEKAAEKKRRREEQKQKDKLKIQKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISCG
                     TLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELI"
     gene            complement(434..1000)
                     /locus_tag="ACHOSM_RS25660"
                     /old_locus_tag="pgaptmp_005132"
     CDS             complement(434..1000)
                     /locus_tag="ACHOSM_RS25660"
                     /old_locus_tag="pgaptmp_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4561406.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGTFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSAYANLSKEM"
     gene            complement(1151..1333)
                     /locus_tag="ACHOSM_RS25665"
                     /old_locus_tag="pgaptmp_005133"
     CDS             complement(1151..1333)
                     /locus_tag="ACHOSM_RS25665"
                     /old_locus_tag="pgaptmp_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561407.1"
                     /translation="MSYHVVEKLFSEYDIRNVVVCFNISISVSISIYIYIYIFISISV
                     SICCDFFFSSCISICF"
BASE COUNT          415 a          322 c          259 g          461 t
ORIGIN      
        1 aatcagttcg acgcgatacg gaacgagatt tccgcttttg tgctggttgc acaccacgca
       61 ttgcttgtga atattgcgtt catcaaatcg gagttgaggt gccgcagcag ttgtccggta
      121 atgtccggca tcccactgag cagacgtgag cgttccgcac gagatacatg gtaagtcgcg
      181 gtctctttct ctgatgaagg cgtttacggc ttgttgggct tgtttaatcc agtaactgcg
      241 gggctttaag gcgagttttt gaatcttaag tttatctttc tgtttctgct cctctcgtcg
      301 tcgtttcttc tctgctgctt tttccgcttt ttcgcgttct ttacttcgtc gttcgagtgc
      361 tatcttggtt ccacaaatct cattacacaa atattgattt tgatattttg gtataaacca
      421 ttcattgcaa catttacatt tccttcgata gattcgcata agcgctcctt tcgttgccgg
      481 aaaaatcacc gtaatactta tctcgggctt cttcagcaac tagtaccgct aactccagat
      541 catcaaagca tccgaagtgt ttactcttgc catggaatcc tagcctaaca ttccattttt
      601 tctgtcgttt gtgccaagta acccctctgc aacctgattt gctattcttt cggatcctta
      661 tatttcttga attttctatt ggcaggcatt ctcttaaatt ttctggccta ttgtcggtcc
      721 taattccatt aacgtggtca atttgaccag caggccaacg attatgagtt atgtaaaaaa
      781 ctaagacgtg agttttatat ctacgcccat ctatcatgat cattgaataa ccgttggaat
      841 caaaagttcc agcaacacta tttaatgcta tccttccctg agtgggaact ttccatctaa
      901 ataccccagt agatttatcg aaacttagta actcaaatat ccttttaaca gttaaatctt
      961 ctcttttacg gttacatcgt cttcgcgctg gtttagccat cgccttcttc ctccgtaatg
     1021 gttttctgaa tttggccacc tgaacagagc tcaccaaagc tatggatgtc ggtatttcca
     1081 caataccaag atggcgaaaa taactgcatg ataagcctca gggaaaaggg aagacactac
     1141 ccccgataat tcagaaacaa atcgaaatac atgaactaaa gaaaaaatca caacaaatag
     1201 aaacggaaat agaaataaaa atataaatat aaatataaat agaaatagaa acagaaatag
     1261 aaatattaaa acagaccacc acattcctga tgtcatactc actaaacaat ttttcgacaa
     1321 catggtagct catagcacgt tatcgtgtag acaccctctg ctacgtattc ggtgttcagt
     1381 gcaatatcta caaatactgg aaaaatctaa aatcgaggca agtttttagt gataactata
     1441 gttagactat attgacg
//
LOCUS       DAZKHC020000315         1419 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZKHC020000315 DAZKHC020000000
VERSION     DAZKHC020000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.317"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(53..214)
                     /locus_tag="ACHOSM_RS25670"
                     /old_locus_tag="pgaptmp_005134"
     CDS             complement(53..214)
                     /locus_tag="ACHOSM_RS25670"
                     /old_locus_tag="pgaptmp_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HGU4561408.1"
                     /translation="MKHPHDNIRVGAITFIYSVTKRGWVFPGLSVIRNPLKAQRLAEA
                     INNKRGLYD"
     gene            complement(211..891)
                     /locus_tag="ACHOSM_RS25675"
                     /old_locus_tag="pgaptmp_005135"
     CDS             complement(211..891)
                     /locus_tag="ACHOSM_RS25675"
                     /old_locus_tag="pgaptmp_005135"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HGU4561409.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTK
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFKAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMANFDEMVPEFIEKMDVALAEIG
                     FVFGEQWR"
     gene            complement(888..1085)
                     /locus_tag="ACHOSM_RS25680"
                     /old_locus_tag="pgaptmp_005136"
                     /pseudo
     CDS             complement(888..1085)
                     /locus_tag="ACHOSM_RS25680"
                     /old_locus_tag="pgaptmp_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
     gene            1077..1226
                     /locus_tag="ACHOSM_RS25685"
                     /old_locus_tag="pgaptmp_005137"
                     /pseudo
     CDS             1077..1226
                     /locus_tag="ACHOSM_RS25685"
                     /old_locus_tag="pgaptmp_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
     gene            1223..>1419
                     /locus_tag="ACHOSM_RS25690"
                     /old_locus_tag="pgaptmp_005138"
     CDS             1223..>1419
                     /locus_tag="ACHOSM_RS25690"
                     /old_locus_tag="pgaptmp_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGU4561410.1"
                     /translation="MNIYERIDGSKYRNIWVAGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELIT"
BASE COUNT          357 a          359 c          353 g          350 t
ORIGIN      
        1 tggtgtgcgc cagctgtcgc gatgttcggc aggagtattg cttccggtga aatcagtcat
       61 acagcccccg tttattattt atcgcctcag ccagccgctg tgctttcagt ggatttctga
      121 taacagaaag gccgggaaat acccagcctc gctttgtaac ggagtagatg aaagtgatcg
      181 cgcctacccg gatattatcg tgaggatgct tcatcgccat tgctccccaa atacaaaacc
      241 aatttcagcc agtgccacgt ccattttttc gatgaactcc ggcaccatct cgtcaaaatt
      301 cgccatgtac ttttcattcc gctcaatcac gacataatgc aggccttcac gcttcatacg
      361 cgggtcatag ttggcaaagt accaggcatc ttttcgcgtc acccacatgc tgtactgcac
      421 ctgggccatg taagccgatt ttatggcctt gaaaccaccg agccggaact tcatgaaatc
      481 ccgggaggta aacgggcatt tcagttcaag gccgttgccg tcactgcata aaccatcggg
      541 agagcaggcg gtgcgcatac tttcgtcgcg atagatgatc ggggatttag taacattcac
      601 gccggaagtg aattcaaaca gggttctggc gtcgttctcg tactgttttc cccaggccag
      661 cgccttagca ttaacttccg gagccacacc ggtgcaaacc tcagccagca gggtgtggaa
      721 gtaggacatt ttcatgtcag gccacttctt tcctgagcgg ggctttgcta tcacgttgtg
      781 aacttctgaa gcggtgatga cgccgagccg taatttgtgc catgcatcat ccccctgttc
      841 gacagctctc acgtcgatcc cggtacgctg caggataatg tccggtgtca tgctgccacc
      901 ttctgctcag tggctttctg tttcagtaat ccaagagctt tcactgcttc ggcctgtgtc
      961 agttctgacg atgcgcgaat gtcgcggcga aatatttggg aacagagcgg caataagtcg
     1021 tcatcccatg ttttatccag ggcaatcagc agagtgttaa tctcctgcat ggtttcatcg
     1081 ttaaccgaat cgggcaggaa gcagtagacg aaatcgaatc aaaccataac cgctatcgct
     1141 ggactgtcga agagtgcaag gcgatcaagg cagagtacca acagaaactc aaagacctgc
     1201 gaaatagcag aagtgaggtt gcatgaatat ctacgaaaga attgatggca gcaaataccg
     1261 aaatatttgg gtagctggcg atctgcacgg atgctacacg aacctgatga aaaaactgga
     1321 gacgatagga ttcgacacca aaaaagacct gcttatctcg gtgggcgatt tggttgatcg
     1381 cggtacagag aacgtagaat gcctggaatt aatcacatt
//
LOCUS       DAZKHC020000316         1413 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZKHC020000316 DAZKHC020000000
VERSION     DAZKHC020000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.318"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(100..480)
                     /locus_tag="ACHOSM_RS25695"
                     /old_locus_tag="pgaptmp_005139"
                     /pseudo
     CDS             complement(100..480)
                     /locus_tag="ACHOSM_RS25695"
                     /old_locus_tag="pgaptmp_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(482..751)
                     /locus_tag="ACHOSM_RS25700"
                     /old_locus_tag="pgaptmp_005140"
                     /pseudo
     CDS             complement(482..751)
                     /locus_tag="ACHOSM_RS25700"
                     /old_locus_tag="pgaptmp_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            954..1034
                     /locus_tag="ACHOSM_RS25705"
                     /old_locus_tag="pgaptmp_005141"
                     /pseudo
     CDS             954..1034
                     /locus_tag="ACHOSM_RS25705"
                     /old_locus_tag="pgaptmp_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            1193..1413
                     /locus_tag="ACHOSM_RS25710"
                     /old_locus_tag="pgaptmp_005142"
                     /pseudo
     CDS             1193..1413
                     /locus_tag="ACHOSM_RS25710"
                     /old_locus_tag="pgaptmp_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG"
BASE COUNT          364 a          294 c          318 g          437 t
ORIGIN      
        1 aattatttgt aaacataata ataagtatta tgacgttaaa atttaaccat acggtaaaat
       61 gattcaggaa aaccggttca gcatagccta ggctgaactt ttgaggatat caacgaacgg
      121 aatttttgaa catttatgag tagatcgttg ttgaagggct tcaatgagca tgtcaagttc
      181 atcaactgct ggtctacgat tacgacggtt tgatttacca atcaaagcaa gtttaagtag
      241 atatggacga gcacttttcg ccgggtttga tgtgtaatta attccgtata taggtttggc
      301 tgcatccaga acactgccaa gataactaac atcgtgactg actgttgctg gacctgcacc
      361 agcgttgttt ctcagcctgc aatgttcaat tacgtcattt tctgtcagtt cagatagttt
      421 gatcgcggag atgtcactat ccataagcag ttctagcaca tatcttttag tggatttgcc
      481 cctatatttc cagacacctg ttatcactta acccattact ggcttgctgc cgtagatatt
      541 cccgtggcga gcgataaccc agtgcactat gcggatgcca ttcgttataa tgctcgaacg
      601 cctctgcaag gttctttgct gccgttaacc cgtctggttt gggcatgaca ctgatgtagt
      661 cacgctttat cgttttcacg aagctctctg ctatgccgtt actctccgga ctccgcaccg
      721 ccgtgctctt cggttcaagc cccaacatct gtagtggtag tacggtctgc tttacctccg
      781 gcatttggtc atttaaatat ttgtgtagta agtcacggac tgtaagtccg tcaacagcat
      841 ttgatgatga aatgccatat agatctaata ccatcacttt ctgtgtgccc catgttttag
      901 catgagcatg aacgcggcgg ctatctcacg ggtgatcatc cctttggcat actgaaaagt
      961 atcgggtgtt actgaaccgt gttgatacat caactgcact ggatattgag tggccgataa
     1021 taccgacgtt ataacctata aatgccaatc agactgaatg ttgaaaggat agaatgaaca
     1081 gcccgacaca caaaaaacgg agtccggctc cggtttttgt gttgccatat aaggcagatg
     1141 tttgttacag ctatttaagt ctggagttca aattaaaata gggagttttg ttatgccatt
     1201 aacctcagat attggatcac gttcatttaa tcttgggctg gaggtttttc gtgcccgaat
     1261 tgcagccaat gggcgcggag atattacagt aggtggtgaa actgtcagta ttgtgtatga
     1321 tgctactaat gggcgctttt catccagcgg gcggtaatga cggattgctt tctgagttat
     1381 tgattttggg atttaataat ggtcctcgag ccc
//
LOCUS       DAZKHC020000317         1401 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZKHC020000317 DAZKHC020000000
VERSION     DAZKHC020000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.319"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            27..1396
                     /locus_tag="ACHOSM_RS25715"
                     /old_locus_tag="pgaptmp_005143"
     CDS             join(27..507,507..1396)
                     /locus_tag="ACHOSM_RS25715"
                     /old_locus_tag="pgaptmp_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HGU4561411.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLVY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RIGEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGILSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQ
                     TYMPRV"
BASE COUNT          421 a          285 c          339 g          356 t
ORIGIN      
        1 gacgatgaaa tggaatagcc cctgatatgt caaagccaaa ataccctttt gaaaagcgcc
       61 tagaagtcgt gaatcactac ttcactactg atgatggtta caggatcatc tcggcacgtt
      121 ttggtgtccc ccgaacccag gtcaggacat gggttgccct ctatgaaaaa catggagaaa
      181 aaggtttaat tcccaaacct aaaggtgtta gtgctgatcc tgagttgcgt attaaggtcg
      241 tgaaagctgt gatcgagcag cacatgtccc ttaatcaggc tgctgctcac tttatgcttg
      301 ctggtagtgg ttctgtagcc aggtggctga aggtttatga agagcgcgga gaagctggtt
      361 tacgcgcgct caagattggc accaaaagaa acattgcaat atcagttgat ccagaaaaag
      421 cggcatcagc attggagctg tcaaaagacc gacgcattga ggatcttgaa aggcaagttc
      481 gatttcttga aacgcggctt gtgtatctaa aaaagctgaa agccttagct catcccacga
      541 aaaagtgaaa gtactcaacg agctaaggca gttttatcct cttgatgagc ttctcagggc
      601 tgcggagata ccgcgcagta cgttttatta ccatctaaag gctctcagca agcctgacaa
      661 gtatgcggac gttaaaaagc gtattggtga gatttatcat gagaacagag gccgatacgg
      721 ataccgtagg gtaacgctgt ctcttcatcg agaagggaaa cagattaacc ataaagctgt
      781 tcagcgcctg atgggaatcc tctcactgaa ggcagcgatt aaggtcaagc gataccgctc
      841 ttacagagga gaggtagggc aaaccgcccc caatgttctc caaagagatt tcaaggctac
      901 gcggccaaac gagaagtggg ttaccgatgt tactgaattt gcagtcaatg ggcgcaagct
      961 gtatttgtct ccagtaatag atctcttcaa caacgaagtt atttcttaca gcctttcgga
     1021 aagaccagtg atgaacatgg tcgagaatat gctcgatcag gcattcaaaa agcttaatcc
     1081 tcacgagcat cctgttctgc actctgacca gggatggcag tatcgtatga gaagatatca
     1141 aaatatcctt aaagaacatg gtattaaaca aagcatgtcc agaaaaggca attgtctgga
     1201 taatgctgtg gtggagtgtt tctttggaac cttaaagtcg gagtgttttt atcttgatga
     1261 gttcagtaat ataagcgagc tgaaggatgc tgttacggaa tatattgaat actacaacag
     1321 cagaagaatt agcctgaaat taaaaggtct gactccaatt gaatatcgga atcagaccta
     1381 tatgcctcgt gtttaactgt c
//
LOCUS       DAZKHC020000318         1392 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZKHC020000318 DAZKHC020000000
VERSION     DAZKHC020000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.320"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..416
                     /locus_tag="ACHOSM_RS25720"
                     /old_locus_tag="pgaptmp_005144"
     CDS             <1..416
                     /locus_tag="ACHOSM_RS25720"
                     /old_locus_tag="pgaptmp_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU4561412.1"
                     /translation="KLSRSAYSDKLTAAFQSHLDETHGQIERIDQVVDSEDGLKLKRI
                     KCAAMEGLIEEANEVIESTDKNEVRDAALIAAAQKVEHYEIASYGTLVTLAEQLGYKK
                     AAKLLKETLEEEKATDVKLTDLAFNNVNKKAQDNS"
     gene            453..959
                     /locus_tag="ACHOSM_RS25725"
                     /old_locus_tag="pgaptmp_005145"
     CDS             453..959
                     /locus_tag="ACHOSM_RS25725"
                     /old_locus_tag="pgaptmp_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU4561413.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAEKMLESMASRIENYPELRSRIEQ
                     HISETKNQLSQLESILDRNNISRSVIKDSMSKMAAFGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASVPIIEAILNEEKQMAEWLLNHIPDTTEQFMVRSE
                     IDGVEAKK"
     gene            978..>1392
                     /locus_tag="ACHOSM_RS25730"
                     /old_locus_tag="pgaptmp_005146"
     CDS             978..>1392
                     /locus_tag="ACHOSM_RS25730"
                     /old_locus_tag="pgaptmp_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HGU4561414.1"
                     /translation="MFRHVKKLQYTVRVSEPNPGLANLLLEQFGGPQGELAAACRYFT
                     QGLSDEDAGRRDMLMDIATEELSHLEIIGSLVGMLNKGAKGALAEGTENEAELYRSLT
                     QNGNDSHITSLLYGGGPALTNSAGVPWTAAYVDTIG"
BASE COUNT          461 a          259 c          304 g          368 t
ORIGIN      
        1 ctaaacttag tcgctctgca tatagtgata aattaaccgc tgcttttcag tcacatcttg
       61 atgagacaca tggtcaaatt gaacgcatcg atcaggttgt tgattctgaa gatggtctta
      121 aattgaaaag aattaagtgt gccgcaatgg aaggacttat cgaagaggcg aatgaggtaa
      181 ttgaaagcac tgataaaaat gaagtacgtg atgctgcatt gattgctgca gcacaaaaag
      241 tagagcacta tgaaattgcc agttatggca ctttggtaac actcgctgag caattaggat
      301 ataaaaaagc ggctaaactc ctgaaagaaa cactggaaga ggaaaaagct actgatgtca
      361 aattaacaga cctggcattt aataacgtta ataaaaaagc tcaggataac tcctgaacta
      421 tagacttcaa tttcttttag gataaacaga ttatgaatag aattgaacat taccatgact
      481 ggcttcgtga tgcacatgcg atggagaaac aagctgaaaa aatgcttgag tctatggcca
      541 gccgtataga aaactatcca gagttacgtt ccagaattga gcagcacatt agcgaaacaa
      601 agaaccagct ttcacagctt gaaagcatac ttgatcgtaa taatatttcg cgctctgtca
      661 ttaaagactc tatgagtaaa atggccgcgt ttgggcaatc cattggtgga atttttccat
      721 ctgatgaaat tgtcaaaggt tcgatcagtg gctatgtgtt cgaacaattt gaaattgcgt
      781 gttacacatc tcttctcgca gcagccaaaa atgcgggtga tacagcttca gtacccatta
      841 ttgaagcaat tcttaatgaa gaaaaacaaa tggctgaatg gcttcttaac catattcctg
      901 acaccactga gcaattcatg gtcagatctg aaattgatgg agttgaagcc aaaaaataat
      961 tcttttgagg aggcattatg tttagacacg tgaaaaaact tcaatataca gtacgtgtaa
     1021 gtgagccaaa cccaggcttg gctaatctac tcttagaaca attcggtggg ccacagggtg
     1081 agcttgcagc agcatgccga tacttcactc aaggcttaag tgatgaagat gcaggccgaa
     1141 gggatatgct gatggacata gcaacagaag aactaagtca tctcgaaatt attggttctc
     1201 ttgttggtat gctcaacaaa ggtgcaaaag gagcacttgc tgaaggaact gagaatgaag
     1261 ctgagcttta ccgttcctta actcaaaatg gcaatgatag ccatataact tctctcctct
     1321 acggaggagg cccagcatta actaattcag ctggagtacc ctggactgca gcttatgttg
     1381 atactattgg cg
//
LOCUS       DAZKHC020000319         1385 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZKHC020000319 DAZKHC020000000
VERSION     DAZKHC020000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.321"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..269
                     /locus_tag="ACHOSM_RS25735"
                     /old_locus_tag="pgaptmp_005147"
     CDS             <1..269
                     /locus_tag="ACHOSM_RS25735"
                     /old_locus_tag="pgaptmp_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4561415.1"
                     /translation="NILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENGI
                     IPEQARVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(415..1005)
                     /gene="tccP2"
                     /locus_tag="ACHOSM_RS25740"
                     /old_locus_tag="pgaptmp_005148"
                     /pseudo
     CDS             complement(415..1005)
                     /gene="tccP2"
                     /locus_tag="ACHOSM_RS25740"
                     /old_locus_tag="pgaptmp_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
BASE COUNT          387 a          246 c          371 g          381 t
ORIGIN      
        1 tgaatatact aaaaaaactt atgcagcgtc tgtgtggttg cggaaagcat gatggccgtg
       61 aacacgtgca gtcgcttaca gcacagctgc gactgggacc ggcagacatt ctggagtccg
      121 atgagaatgg cattatcccg gagcaggcca gggtaatcac gcaggtggtg atactggatg
      181 cggataaaaa gcagatacag tgtgtggtaa gaccgctgca aatcctgcgt gctgacggga
      241 cgtgggaaaa tattggcgga atgaaatagc cgacagcttc acaaaaaccg gagtccggct
      301 ccggtttttg ttggttagat gtaatctgac agatacctgt ataaataacc ggtaactgtc
      361 aggtcagagc taatacaggt aattatatta taatcacgag cgcttagatg tattaatacc
      421 atgctctgca agatgctgca tcagacgctg agccacatca ggcagaggtc ttgattgttc
      481 attttgtacc ggcggcgttg gcgcaggcca gttaggtgcc ggaggaatat attcagccat
      541 attccgggct ggttgaatgc catgctctgc aagatgctgc atcagacgct gagccacatc
      601 aggcagaggt cttgattgtt cattttgtac tggcggtggt ggcgcaggcc agttaggtgc
      661 cggaggaata tgttcagcca tattccgggc tggttgaatg ccatgctctg caagatgctg
      721 caccagacgc tgagccacat caggtagagg ccgatgtaaa gcgttttttt ctatacttga
      781 ctgagtataa gatgtgggac gttgaagtcc tgattctgta taagaatttt ttaccttaaa
      841 aatagttgtt gcactggcag gactgttgct gggagaaaaa ggtgagctgg tttttaccgt
      901 gttaggtgtg cttttgtgct gcgagacagt gaaggtactg ctgcgtatag cacttgatat
      961 actgcgagga aggctggaaa aaaaagaatt aatgctattt atcatataga taaccttata
     1021 agagctttat tggatagtct tatttatccg tttacatgaa atctaccttt tgtgaaattc
     1081 tcgtcaatat tttactcaaa atgatcggct gtaggaataa actatttgat attattacgt
     1141 ttttagagtt gttttcaatc agctatagat agggcttggc gccagaatgg agaatattta
     1201 acgaggaata gaagctttgt atcagacggg cttccctaaa tagagaagtt aaataatcag
     1261 gatgatgcta atgaatatca gggacaaggt gagtgtcttc atgttcagtg aaacagggtg
     1321 tcacaaaaag attggggagg gctagagcct tctgcacgaa aatagtgatg cgacagatct
     1381 gagat
//
LOCUS       DAZKHC020000320         1374 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZKHC020000320 DAZKHC020000000
VERSION     DAZKHC020000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.322"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /locus_tag="ACHOSM_RS25745"
                     /old_locus_tag="pgaptmp_005149"
     CDS             <1..198
                     /locus_tag="ACHOSM_RS25745"
                     /old_locus_tag="pgaptmp_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4561416.1"
                     /translation="WGISGEAELAVMQAIAEKPGALRVLTHTLNQAWLTASGEGAALT
                     EKHINAAFKEVYTNPELLSQV"
     gene            201..422
                     /locus_tag="ACHOSM_RS25750"
                     /old_locus_tag="pgaptmp_005150"
     CDS             201..422
                     /locus_tag="ACHOSM_RS25750"
                     /old_locus_tag="pgaptmp_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561417.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATDVYSGICRSVGAKQVS"
     gene            435..689
                     /locus_tag="ACHOSM_RS25755"
                     /old_locus_tag="pgaptmp_005151"
     CDS             435..689
                     /locus_tag="ACHOSM_RS25755"
                     /old_locus_tag="pgaptmp_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561418.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            691..972
                     /locus_tag="ACHOSM_RS25760"
                     /old_locus_tag="pgaptmp_005152"
     CDS             691..972
                     /locus_tag="ACHOSM_RS25760"
                     /old_locus_tag="pgaptmp_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HGU4561419.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            969..1241
                     /locus_tag="ACHOSM_RS25765"
                     /old_locus_tag="pgaptmp_005153"
     CDS             969..1241
                     /locus_tag="ACHOSM_RS25765"
                     /old_locus_tag="pgaptmp_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561420.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKARALMKDMNMSSGVIKGNEFNQQG"
     gene            1246..>1374
                     /locus_tag="ACHOSM_RS25770"
                     /old_locus_tag="pgaptmp_005154"
     CDS             1246..>1374
                     /locus_tag="ACHOSM_RS25770"
                     /old_locus_tag="pgaptmp_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561421.1"
                     /translation="MSKVRVIFEFEHVSHDEKPAGNDSVEVHEKIGVDVKTERDTDN"
BASE COUNT          376 a          278 c          398 g          322 t
ORIGIN      
        1 tgggggatca gtggtgaggc cgagctggcc gtcatgcagg ctatcgctga aaagccggga
       61 gcgttacgcg ttctgacaca tacgcttaac caggcgtggc tcaccgccag cggtgaaggc
      121 gcggcgctga cagaaaaaca tattaatgcg gcctttaaag aggtttatac caaccctgaa
      181 ttactctcac aggtgtgatt atggcggtat ttaatattcc tgatatttac ggacgctttt
      241 acctggttaa tttcgataac gtgaaggtga tttcactggc cgaaaataaa gaatgtggcg
      301 atttactttt tgaatttaat gaccgcacac gaatggtgat atctgccgga cttgatcgcg
      361 aaggtgcgac agacgtttac agcggaatat gccgttctgt tggtgcgaaa caagtcagct
      421 aaatgaggtg ttatatgaat atgcaatcct gcggtaacaa aatgaattta ttcgactccc
      481 tgaacagcgc gcgccgtctg accgaacttg ccggtgcggt actggaacgc agtaagcgct
      541 atccgcaacg ttttgcactg aaaaccacgc cgccggtagg caacgtgcag ggaaccggtg
      601 aaattgaaat caccatacag accaacggcc tgcgccgccg tgtgaaggcc acccgcatca
      661 gcggctgcac ggtttactgg gaggtgtgag gtgaaaaaaa atctcattgc atgggcgtgg
      721 tcgagtggtc ttattgagtt tggttacgtc ctgccggaag gtgcattgcc gatagttgcc
      781 ggaaagcctg ccacggtacg gcatgtgatt gaggttatgg cgcgtcatgg acgtgatgaa
      841 caggagcagt tactggttcc ggggataccg gaagcggtga cggaggaaga agcctttaat
      901 gccatgattc ggttctgccg tgaggtcaga cgccgggtca gttatccaaa cagaacgagg
      961 accagaggat gagtaaagtc gtacgcatta ttttcgaata caaggagcac gttatccata
     1021 aaaacgctga tggaacagtg cgcatggggg taagtctgga catacgttca accgggataa
     1081 agcagaaagg tgatggaccc gccatgattt ttggagtggt tatgctcgcg gaaagcagag
     1141 actttgctga acttgtggca atgaaagcca gagcgctcat gaaagatatg aacatgagtt
     1201 ccggggttat taaaggtaat gaatttaatc agcaggggta attccatgag caaagtacgc
     1261 gttatttttg aatttgagca tgtttcgcat gacgaaaaac cggcaggcaa tgacagtgtt
     1321 gaagtgcatg aaaagattgg agtggatgtg aaaacagaac gtgatacgga taac
//
LOCUS       DAZKHC020000321         1368 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZKHC020000321 DAZKHC020000000
VERSION     DAZKHC020000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.323"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            45..1190
                     /locus_tag="ACHOSM_RS25775"
                     /old_locus_tag="pgaptmp_005155"
     CDS             45..1190
                     /locus_tag="ACHOSM_RS25775"
                     /old_locus_tag="pgaptmp_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EspG domain-containing protein"
                     /protein_id="HGU4561422.1"
                     /translation="MINGLNNNSASLVLDAAIRINSDFKKQWNDMSCAEKLLKVLSFG
                     LWNPTYTRSERQTFQELLTVLEPVSPAPNELGRIYANFADGSSLRISVTNSELVEAEI
                     RTPDNEKILMLLESNEQNRLLQSLPINLHMPYIQVHRALSKMDLTDHKSMHNLLSFTS
                     KLSATLIPHNTQTDPLSGPTPFSSMFMDTFRGLGNAKLSLNGVDIPVDAQKLLRDALG
                     LKDTHSSLARNVINNGISRHHAKQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALMLTHTRISQAREYNVERSLDVPNACINISISQSPDGSIHVASHTGILIMAP
                     EDRPNELGMLTNRTSYEVPQGVKCEIDEMVRTLQPRYGASETYLKNI"
BASE COUNT          465 a          284 c          245 g          374 t
ORIGIN      
        1 ctgttgtcga atcattcata ataaatgaga caggggaatc tctcatgata aacggactta
       61 ataataattc cgcatcttta gttttagatg ctgcaattag aattaattct gattttaaaa
      121 agcagtggaa tgatatgtca tgcgctgaaa aattactaaa agttcttagt tttggtttat
      181 ggaatccaac gtatactcgt agtgaaaggc aaacatttca agagttgtta accgttttag
      241 aacctgtatc tcccgcgcct aatgaattgg gcagaatata tgctaatttt gcagatggct
      301 catccttaag aatttccgtc actaacagcg aacttgttga agccgagatt cgcacaccag
      361 ataatgaaaa gattcttatg ctcctggagt caaacgaaca aaataggtta ttacaatctt
      421 tacccatcaa tctccacatg ccatacattc aggttcatcg tgccttatct aagatggacc
      481 tgactgatca taagtcaatg cacaatctac ttagttttac gtcaaaacta tcagcaacct
      541 tgattcctca taatactcaa acagatccgc tttccgggcc tacaccattc agctctatgt
      601 ttatggatac atttcgagga ctcggcaatg caaagctttc actcaatggt gttgatatac
      661 ctgtagatgc acaaaaattg cttcgcgatg cactaggact taaagacaca cattcatcac
      721 ttgcccggaa tgttataaat aatggtattt ctcgtcatca tgcaaagcaa atagcaaggg
      781 aaagcagcgg cagcgataaa cagaaagctg aagttgtgga gtttttatgc caccccgaag
      841 cggcaacagc catatgctcg gctttctatc aatctttcaa tgtgccagcc ttaatgttga
      901 cacatacaag gatctctcaa gccagagaat acaatgtgga aagatcgtta gatgtaccta
      961 acgcgtgcat taacataagt atctctcaat caccagatgg aagcattcac gttgccagcc
     1021 ataccgggat tctgataatg gcgccagaag accgccccaa cgagttagga atgttgacga
     1081 acaggacctc ctatgaagtg ccacaaggtg tgaaatgtga aatcgatgag atggtaagaa
     1141 cgctacaacc aaggtatggc gcatctgaaa catacctgaa aaacatttaa aaataaaaaa
     1201 atggacacca cacactttat ttatcaaaac aaacaataac taatcacact cacaattatt
     1261 aataattcaa cctcacagca atcatacaaa acaaagatat ttatttcgca aaccatttat
     1321 agcgaatgga gaaggaatga aaagaatctt atttattgca ctttttat
//
LOCUS       DAZKHC020000322         1368 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZKHC020000322 DAZKHC020000000
VERSION     DAZKHC020000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.324"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..709
                     /locus_tag="ACHOSM_RS25780"
                     /old_locus_tag="pgaptmp_005156"
     CDS             2..709
                     /locus_tag="ACHOSM_RS25780"
                     /old_locus_tag="pgaptmp_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HGU4561423.1"
                     /translation="MYERSQLTQVMISSAPATAETMEKAEYLRLDCTIKEVQFTAGQK
                     QDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQFPS
                     GKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTVNTGA
                     LLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSAEQP
                     QSITSDACTVTVNGAGG"
     gene            715..1089
                     /locus_tag="ACHOSM_RS25785"
                     /old_locus_tag="pgaptmp_005157"
     CDS             715..1089
                     /locus_tag="ACHOSM_RS25785"
                     /old_locus_tag="pgaptmp_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU4561424.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWRNVSVVLREPSAEAWYLW
                     KEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            1185..>1368
                     /locus_tag="ACHOSM_RS25790"
                     /old_locus_tag="pgaptmp_005158"
     CDS             1185..>1368
                     /locus_tag="ACHOSM_RS25790"
                     /old_locus_tag="pgaptmp_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HGU4561425.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALED"
BASE COUNT          312 a          320 c          442 g          294 t
ORIGIN      
        1 tttgtatgaa cgttcacagc tgacgcaggt gatgatttca tctgccccgg cgactgctga
       61 aaccatggag aaggcggaat atctgcgcct ggactgcacc atcaaggaag tccagttcac
      121 cgccggtcag aaacaggata ttgatgtgac cacgctctgc tccacagagc aggagaacat
      181 caacggtctg ggggcgtcgt ccgagatttc catgtcgggt aatttttatc tgaatcaggc
      241 ccagaacgcc ctgcgtgatg cctatgacaa tgacacggtg tatgcgttta aggtgcagtt
      301 tccgtccggt aagggcttta agttcctggc ggaagtgcgt cagcacacct ggtcatccgg
      361 taccaacggc gtggtggctg caacgttttc acttcgcctg aagggtaaac cggtgtccta
      421 tgtggtaccg ctggcgtttg tgaaaaatct ggagaagaca cttaccgtga ataccggtgc
      481 gctgctgaca atgtcagtca gtgtcaacgg gggaacgccg ccttataaac acgcctggaa
      541 gaaggatggt cagccggtag agggacagac tactgacact ttcagtaaag ccaatacgca
      601 gtcaggtgat aagggggctt atacctgcga ggtaacggat tctgcagaac agccgcagag
      661 cattacctct gatgcgtgta cagtaacggt taatggtgcg ggcggataag gcttatggca
      721 aaagatctga aaacactggc gctggccaga ctgtcggggt tccgtcataa aacggtgaag
      781 gtgccggaat ggagaaatgt cagcgtggtg ctgcgggagc cttcggcaga ggcctggtat
      841 ctgtggaagg aagtgctcaa tggtgatgga gaggatgacg ataccctgtc ggtggtggcg
      901 aaaacccgcc gtaacctgga agcggatgtg acgctgttct gcgatgtcct gtgtgatact
      961 gacctgcaac gggtgttcac tccggacgac cgtgagcagg tgctggccgt ctatggtccg
     1021 gtacatgccc ggttgctgcg tcaggcactg gaactgatcg ctgatgcaga gtcggccaga
     1081 aaaaagtagc ccgcccggaa attcgctttc tgatgcgact tgcgctccgt ctggggcgca
     1141 ccttatccga actgcggcac agcctgagtg cgagcgaggc gatgatgtgg atggagttcg
     1201 acagggtatc cccgctgggt gatgagcgcg gggatatccg taatgcacag atcgtgaaag
     1261 cggttttcgg ggcacagggg atgaatgttg cactgaagga cgccatgctc tgctggggcg
     1321 aggatgagga taagccggag gtggatccgt ttgcggcgct ggaagacg
//
LOCUS       DAZKHC020000323         1362 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZKHC020000323 DAZKHC020000000
VERSION     DAZKHC020000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.325"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1362)
                     /locus_tag="ACHOSM_RS25795"
                     /old_locus_tag="pgaptmp_005159"
     CDS             complement(<1..>1362)
                     /locus_tag="ACHOSM_RS25795"
                     /old_locus_tag="pgaptmp_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine protease"
                     /protein_id="HGU4561426.1"
                     /translation="NINVNIDTSDLNITLRQGLDSLTEAIDEMNIDGIMAIVGIIQYM
                     KITGYGGYVSAIDHANFVSDIKTVVEKVVGTSLMFMGVKKFGTNISQIRLETMASITL
                     SQVATRLGGVQGRILSKIANIIKFPVIDTALNLWSLGESIHRYTSEKSGSLDKMLAEI
                     DVAFASTYTALTLSSFAFPPIALATLPLIFLQQDIKTFKVHLHMENERRDAWKKVENY
                     LDTAARRNVVKVDTFRGVIDLSAIDIIGSLKLDLSTENVKLSGNLSYNRGKNIGNNPN
                     LSDIDVRKISKYAVACVNDDDLHVPSFGGGNRKERCRELSLSESGLVRGFANRIWPSY
                     VPMIKKGNYNTVILGYSSQIVANTEVIRMAMHDFQEVARENYPLVEVINKNTEVVTGN
                     KNVKVILPVLEQDIFSPKNSEDLYNLSTHWFTVRGGEKGVTVFPNGVGNFNIIGALGA
                     KNIL"
BASE COUNT          418 a          283 c          195 g          466 t
ORIGIN      
        1 agtatgtttt ttgctcccaa tgcacctatt atattaaaat taccaacccc attggggaaa
       61 acagtaacac ctttttcacc acctctgacg gtgaaccaat gtgtacttaa attatataag
      121 tcttcactgt ttttaggaga gaaaatatcc tgttccaata caggaagaat taccttaaca
      181 tttttattgc ctgtaacaac ctcagtattt ttatttatta cttcaaccaa aggataattt
      241 tctcttgcaa cctcttgaaa atcgtgcatg gccattctaa ttacttctgt attagccaca
      301 atttgagagg aataccccaa tatcacggta ttataattcc ctttttttat catcggtaca
      361 tatgaaggcc atatcctgtt tgcaaaaccg cgaactaatc ctgattctga taaagatagt
      421 tctcggcatc tttcctttct attccccccg ccaaaactag gaacatgcag gtcatcatca
      481 ttaacacaag caacagcata tttacttatt tttctaacat ctatatcact caggtttgga
      541 ttattcccaa tattttttcc tctgttatat gagagattac cggataattt cacattttct
      601 gttgaaagat ctagttttaa actaccaatt atatcaattg ccgaaaggtc tattacaccc
      661 cggaaagtat caactttaac aacattacgt cttgcagcag tatctagata gttctcaact
      721 tttttccaag cgtctcttct ctcattttcc atgtgaagat gtactttaaa cgtctttatg
      781 tcttgttgta aaaaaataag cggcagagtg gctaatgcga tgggtgggaa tgcaaaacta
      841 gatagtgtaa gcgcggtata agttgatgca aaagcaacat ctatttctgc taacatttta
      901 tctaaacttc cacttttctc cgatgtatat ctgtgaatag attcaccaag agaccataaa
      961 tttaatgctg tatcgatgac aggaaactta attatattag ctatcttaga taaaattctt
     1021 ccttgaactc caccaagtct tgttgcaact tgagataacg taatagaagc cattgtttct
     1081 aaacggatct gagagatgtt ggtgccgaat ttttttactc ccataaacat caggctcgta
     1141 cccacaactt tctctactac tgtctttatg tctgacacga aattcgcatg gtctatcgct
     1201 gatacataac caccataccc cgtaattttc atatattgaa taataccgac aatagccata
     1261 atcccatcaa tattcatttc atcaatggct tctgttaatg aatcaaggcc ttgtctgagc
     1321 gttatattta aatctgaagt atcaatatta acatttatat tt
//
LOCUS       DAZKHC020000324         1357 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZKHC020000324 DAZKHC020000000
VERSION     DAZKHC020000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.326"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..66
                     /locus_tag="ACHOSM_RS25800"
                     /old_locus_tag="pgaptmp_005160"
     CDS             <1..66
                     /locus_tag="ACHOSM_RS25800"
                     /old_locus_tag="pgaptmp_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4561427.1"
                     /translation="RYQAKYVSAIWQKLIPSQADF"
     gene            134..376
                     /locus_tag="ACHOSM_RS25805"
                     /old_locus_tag="pgaptmp_005161"
     CDS             134..376
                     /locus_tag="ACHOSM_RS25805"
                     /old_locus_tag="pgaptmp_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGU4561428.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            354..>1357
                     /locus_tag="ACHOSM_RS25810"
                     /old_locus_tag="pgaptmp_005162"
     CDS             354..>1357
                     /locus_tag="ACHOSM_RS25810"
                     /old_locus_tag="pgaptmp_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4561429.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     L"
BASE COUNT          461 a          289 c          318 g          289 t
ORIGIN      
        1 cgttatcagg caaaatacgt ttcagctatc tggcaaaaac tgatcccgag tcaggctgat
       61 ttttaatgtt caacccatat cgccgcccac cagctacagt ggcggcggtc atgctgtaaa
      121 ggcacgtgac cacatgtacg aattaactct atctccagca gagattaaag agatcacgaa
      181 atacgagcga tacacaaaac agcaacacca gttaagactg cacggcatcc catttgtaat
      241 cggtcctaaa aacgaaccaa tagttctacg cagggatatt ccacacggac tgacaacgat
      301 gccaaaaaca tctgaactgg tttctgctga acccgatttt gaggcgctga ataatgggaa
      361 gaccaagaaa aaataaaaaa gataatgtac tgccaccgcg ggttagatcg aatggttaca
      421 gttacgtgtg gaaaccagaa ggaagtacaa gaagtatagg gctaggaaga gtgcggaaaa
      481 ccagcgtagc taaagtctgg caaaattatg aactggaaaa agcaaaactc cacaacataa
      541 tgaccgtagc taaattgtgg cacatgttta tggactcccc tgcatttaca gaactggccc
      601 cccgaaccca aaaagattat cgacaacatc agaaggcgtt gctgatggta ttcggaaaag
      661 tgcttgctga taatgtcaaa actgagcagg taagaatttt catggataaa cgagggcttg
      721 agagcaagac ccaggcaaat catgaactgg caagcctgag tcgagtatac gggtggggat
      781 atgagcgtgg gtatgtgaag aataacccat gcaaaggagt cagaaaattc tctcttaaag
      841 cccgcactgt ttacatcacc gatgaacagt atgcggcgat atatgcggaa gcaattccac
      901 agttacgcat tgcaatggag atttcctatc tctgtgcggc aagactcggt gatgtgcttg
      961 agttgaaatg gcaggatatt atggataaag ggatctacat tgagcaaaac aaaaccggca
     1021 ccaaacaaat caaggaatgg tcaccgcgat tacgtacagc gatccagtta gcccgaaatg
     1081 tatcttcctg tacatgcgaa tatgtgatca atacaaccaa aggcgggaaa gtcatagcta
     1141 aaacgctgaa taactggtgg aatcaggcta aacgcgcagc cgagcaaaaa gttggcgttc
     1201 cgttcgggtg caattttcac gacataaaag ccaaggggat ctcagattac gaaggcagca
     1261 gtcgcgacaa acaaattttc agcgggcata aaacagaaaa tcaggtgttg atttacgatc
     1321 gtaaaacaaa aatcacacca acactggatt tgccgct
//
LOCUS       DAZKHC020000325         1355 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZKHC020000325 DAZKHC020000000
VERSION     DAZKHC020000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.327"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            87..758
                     /gene="queE"
                     /locus_tag="ACHOSM_RS25815"
                     /old_locus_tag="pgaptmp_005163"
     CDS             87..758
                     /gene="queE"
                     /locus_tag="ACHOSM_RS25815"
                     /old_locus_tag="pgaptmp_005163"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HGU4561430.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          363 a          293 c          321 g          378 t
ORIGIN      
        1 tctgcctgta acagaatgtt aataagacat gattgttgtg gcaggatctg caataattaa
       61 caattatcca cttcacagag aatgctatgc agtacccgat taacgagatg ttccagaccc
      121 tgcaaggtga gggttacttt accggcgtcc ccgccatttt tattcgttta cagggatgcc
      181 cggttggctg tgcctggtgc gacaccaaac acacctggga aaagcttgag gatcgggaag
      241 tctccctttt cagcattctg gcgaagacca aagagagtga taagtggggg gcggcgagca
      301 gtgaagactt gttggctgtc attagtcgcc agggatacac cgctcggcat gtggtgatta
      361 cgggtggtga gccttgcatt catgatttgc taccactgac tgatctgctc gaaaagaacg
      421 gttttagctg ccagatcgaa accagtggta ctcatgaggt acgctgcaca ccgaatacct
      481 gggttaccgt atcaccaaag ctgaacatgc gcggcggata tgaagtgttg tcacaggcac
      541 tggagcgagc caacgaaatc aagcatccag tggggcgcgt acgtgatatt gaagcactgg
      601 atgaactact ggcaacgctg accgatgata aaccgcgagt cattgcactg cagccgatta
      661 gccaaaagga tgatgccaca cgtttgtgca ttgaaacctg cattgcgcgt aattggcgtt
      721 tgtcgatgca aacacataaa tatctaaata ttgcctgatt aaacatttat aagcgttata
      781 aatgggtgga acctgtaaag acttctactc atttatattg tttgtcgcct ctgaaaactc
      841 ctccatttta cccatccagg gctaatcatt agcattctct acaaattctg tggcattaat
      901 ttttcgctgg agtgaaaatt attgcggtaa agtttggtag attttagttt gtatagagtt
      961 attttaaata tttacctttt taatcaatgg attaagtgct ctttaacata atggatgtgt
     1021 tgtttgtgtg atactataaa gttggtagat tgtgactggc ttaaaaaatc attaattaat
     1081 aataggttat gtttagagtg ttccccgcgc cagcggggat aaaccgagca gatacacggc
     1141 tttgtattcc gtgcgcccgt gttccccgcg ccagcgggga taaaccggac gccgccgccg
     1201 cgaagccgtt tccgatgttg agttccccgc gccagcgggg ataaaccacc atataacccg
     1261 ttatctcttt ctcaagtttt tatattagca gtacttgtaa taagcaacat atccacgtaa
     1321 cacctcatgt tcaaaatagt tctccatgcc agtga
//
LOCUS       DAZKHC020000326         1352 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZKHC020000326 DAZKHC020000000
VERSION     DAZKHC020000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.328"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(98..682)
                     /locus_tag="ACHOSM_RS25820"
                     /old_locus_tag="pgaptmp_005164"
     CDS             complement(98..682)
                     /locus_tag="ACHOSM_RS25820"
                     /old_locus_tag="pgaptmp_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4561431.1"
                     /translation="MTTFTKEQLISHVSENVKAMKFAVKQTAFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     KKGSSTNNFREISETSTNYPVTLDGWISCSERMPDDCQHVIILCDGAFVLYAQYRDGE
                     FFDVVRDGDEFFETQSRNVTDWMPLPEPPQEVRQ"
     gene            complement(739..1134)
                     /locus_tag="ACHOSM_RS25825"
                     /old_locus_tag="pgaptmp_005165"
     CDS             complement(739..1134)
                     /locus_tag="ACHOSM_RS25825"
                     /old_locus_tag="pgaptmp_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU4561432.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKTTDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAVL"
     gene            complement(1150..>1352)
                     /locus_tag="ACHOSM_RS25830"
                     /old_locus_tag="pgaptmp_005166"
     CDS             complement(1150..>1352)
                     /locus_tag="ACHOSM_RS25830"
                     /old_locus_tag="pgaptmp_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4561433.1"
                     /translation="PLPSFTETQADELIFPSLRRANLALRRAKSDVQKWERVCAALRE
                     LNKHRDIVRQIVDSSGRIVSEK"
BASE COUNT          328 a          377 c          283 g          364 t
ORIGIN      
        1 aatcccggcg aacgttttta cccccaccga caaataacat atactaaaaa agcgatagcc
       61 atagcaacgc ctgtaattgc aaatgcttca ggccagttca ttggcgcacc tcctgcggcg
      121 gttctggtag cggcatccag tcggttacat tgcggctctg tgtttcgaaa aattcatcac
      181 catcacggac tacatcaaaa aactcaccgt ctcgatattg cgcataaaga acgaatgcgc
      241 catcacataa aataattacg tgctgacagt catccggcat tcgctcacta cagcttatcc
      301 aaccatccag agttaccgga tagttggttg acgtttccga gatttcccga aaattattgg
      361 ttgacgaacc cttcttttcc cgaaagtttc cagcctgaag catggcggcg ctgtctggcg
      421 gggcagcata tagcggcacg tatatttccg gttccttatc agcaccgggt tgctcttcca
      481 gtgagaatgt ctttccggta aatcgattca tataaagcac gggctctgct tccagcgaag
      541 cctgagcaac aagggccagt gctaaatcca actcaattgc ctcgagagaa tttttgaatg
      601 ctgtctgttt tactgcaaat ttcatcgcct ttacattttc actaacatga ctgattaact
      661 gctcttttgt aaaagtggtc atctcattct cctttgatgc gaatgccagc gacaattgaa
      721 gcctgatagc taattcactc acagtaccgc ctcctgaaaa ttgccctgat agaacgccag
      781 tacacgcagc ataacttcac tcttccggca ctcgccacag attatgttct gttgtctgtc
      841 gtagcggcgt atttctccgt ctggtaactt tcgaatcaat gtctggtcgg ttgttttctc
      901 cgctgcctta cgccatacgc gatacacctg ttctgatgta aaaacaccgt atttaccggg
      961 catgtataaa tcgccacaag ccagtacatc cacaaggcaa cgtctgactg aatgccagcc
     1021 tgctcccgtc gctctctcca gttgtgatat cgtcatgcgt tcatttttgc gtaccagccc
     1081 gataattcgg gccttcagtt cttcacgctg ttcgtgtgta aaaggtttcg ccataagcgc
     1141 ctccggcaat cacttttccg acacaatacg accggatgaa tcgacaatct gccgaacaat
     1201 atcccggtgc ttgttcagct cccgcagcgc ggcgcagact cgctcccact tctgaacatc
     1261 acttttcgcc ctgcgcagcg ccaggtttgc cctgcgaagg gacggaaaaa tcagctcatc
     1321 tgcttgcgtt tcggtaaacg atggcaacgg ct
//
LOCUS       DAZKHC020000327         1347 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZKHC020000327 DAZKHC020000000
VERSION     DAZKHC020000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.329"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            289..>1347
                     /locus_tag="ACHOSM_RS25835"
                     /old_locus_tag="pgaptmp_005167"
     CDS             289..>1347
                     /locus_tag="ACHOSM_RS25835"
                     /old_locus_tag="pgaptmp_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4561434.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWGYHDNTTTDLV
                     WPYDTDNDGIADAGGMDPMGSDGNYVTYNGFVYYNNANGDFDTVFKGDTVNGTISTYY
                     LNHDYASGSVNQVDISNSVIHGSITSELPFGYYSITPEGYNGYTFNNGTTTDLDYNWY
                     DGDVFTLNVSNSTIDDDYEALYFTDTHLDGDKSKSTNETFYTALGVAVNLDVESNINI
                     TNNSRVAGIALSEGNTSNTTYTSEYHQWDNNINVSNSTVTSGSQTPLEESDGFFGKSA
                     EPSDYAGNGGQNDVALSFTDNAGSNYSMKNNVNFDHSTLLGDVEFTSHWNNDGVFFYS
                     TGHDSNGDGVLDTNGGWVD"
BASE COUNT          396 a          276 c          271 g          404 t
ORIGIN      
        1 actttgttgt atattgataa gatatttatc tggtttgtgt ttttgatatt tatagcgcgt
       61 atgtatcaaa aataacatta tctgcaacat gttgaaacat cgtcctttta tcaattcgat
      121 gcgtgttttt ctccataaat tatatatgtc cacattcgga cttaggggaa agaataattg
      181 aaccattcgt ctgtaacgca gcataatcgt tagcgcgaaa cataatatgt tttctatgca
      241 ttgataattg atggatcaac ttattacgtc cctgaggagg gatgacaaat gcactcctgg
      301 aaaaagaaac ttgtagtatc acaattagca ttggcttgca ctctggctat cacctctcag
      361 gctaatgcag caaactatga tacctggggg tatcatgaca acaccacgac cgatttagtt
      421 tggccgtatg acaccgacaa tgatggaatt gccgatgctg gcggcatgga cccgatgggt
      481 tcagatggta actacgtaac ttacaatggt tttgtttact ataacaatgc caatggcgat
      541 ttcgataccg tctttaaagg cgataccgtt aacggtacca tttctactta ttacttgaat
      601 catgattacg ctagcggttc tgttaaccag gtagatatca gcaattcagt aattcatggg
      661 tcaatcactt ctgaactgcc atttggttat tacagcatta cgcctgaagg ttataatggc
      721 tatactttca ataacggaac tactactgat cttgactaca attggtacga tggtgacgta
      781 ttcactctga acgtatccaa ttccaccatt gatgatgact atgaagctct ttacttcact
      841 gacacacatc tcgatggtga taaatctaag tcaaccaatg aaacgttcta taccgctctg
      901 ggtgttgcag taaatcttga tgttgaaagc aatatcaata ttaccaacaa ctcccgtgta
      961 gctggcattg cgctgtctga aggcaataca agcaacacaa cttatactag cgaatatcat
     1021 cagtgggaca acaacatcaa tgtgtctaat tccactgtaa cttcaggatc acagactccg
     1081 ctggaagaaa gtgatggctt cttcggtaaa tctgctgaac caagtgatta cgctggtaat
     1141 ggtggccaga atgacgtagc actgtcattc actgacaatg ctggctcaaa ttattccatg
     1201 aaaaacaatg taaactttga ccattcaact ctgttgggtg atgtcgaatt tacaagtcac
     1261 tggaataacg atggcgtatt cttctattcc accggtcatg acagcaacgg cgacggcgtc
     1321 cttgatacca acggcggttg ggtagat
//
LOCUS       DAZKHC020000328         1347 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZKHC020000328 DAZKHC020000000
VERSION     DAZKHC020000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.330"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..569
                     /locus_tag="ACHOSM_RS25840"
                     /old_locus_tag="pgaptmp_005168"
     CDS             <1..569
                     /locus_tag="ACHOSM_RS25840"
                     /old_locus_tag="pgaptmp_005168"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGU4561435.1"
                     /translation="YFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYL
                     KLQALFARVAEEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVP
                     YTDVWTHKPVQYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALG
                     RRATGVELETERFEQTVREVQDLVSQNG"
     gene            615..685
                     /locus_tag="ACHOSM_RS25845"
                     /old_locus_tag="pgaptmp_005169"
     tRNA            615..685
                     /locus_tag="ACHOSM_RS25845"
                     /old_locus_tag="pgaptmp_005169"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:643..645,aa:Met,seq:cat)
     gene            complement(789..923)
                     /locus_tag="ACHOSM_RS25850"
                     /old_locus_tag="pgaptmp_005170"
                     /pseudo
     CDS             complement(789..923)
                     /locus_tag="ACHOSM_RS25850"
                     /old_locus_tag="pgaptmp_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1164..>1347
                     /locus_tag="ACHOSM_RS25855"
                     /old_locus_tag="pgaptmp_005171"
     CDS             1164..>1347
                     /locus_tag="ACHOSM_RS25855"
                     /old_locus_tag="pgaptmp_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4561436.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVV"
BASE COUNT          320 a          302 c          395 g          330 t
ORIGIN      
        1 cttactttcg tgatgcgcgt gctgccctgg ggataacggc aaaacagata gtggatgcca
       61 caggaaagaa aaacatggtg tcgcactggt tcagtgccag tcagtggcag ctaccgaacg
      121 aaagcgatta tctgaaatta caggcgctgt ttgcccgggt ggcagaagag aagcatcagc
      181 gcggtgaact ggaaaagccc caccaccagc tgctggagac gtatacttca ctgaaccggc
      241 agtatgcgga actgcagagt gaatataaac atctgcggcg gtattttggc gtgacggcgc
      301 aggtgccgta cacggatgtg tggacgcata aaccggtgca gtactatccc gggaaacatc
      361 cgtgcgaaaa accggcagaa atgctgcagc agataatcag tgcgagcagt cgtccgggtg
      421 acctggttgc agatttcttc atggggtcgg gttcgacagt caaagccgcg atggcgctgg
      481 ggcgtcgtgc aactggcgtt gagctggaga ctgaacgttt tgagcagacg gtcagggaag
      541 tacaggattt agtcagtcag aacggatgat attgcaggat tagttacgta ccgttattat
      601 cctgcgcccg gccctttagc tcagtggtga gagcgagcga cgcataatcg ccaggtcgct
      661 ggttcgaatc cagtacaacg cgccacactt attttccctg gctcgctttt gcgggccttt
      721 ttttaaatgt ctcacaattc aggcggttga ctgttgtctg gtttgcgggg agtttgttaa
      781 aagaaactgg catggtgaat ccccctgtgc ggaggggcaa tcagcaacca ggtatatggg
      841 gtaatcgcgg attcaggtgc tggtactgaa ttcaccggga ggcacccggc accatgcaag
      901 aaaaagaatg tgcatgcaaa catgcccctc tccggagggg cttttttatg ggtaaaaaaa
      961 tgcccgaatg ggttcgggca atagcatgag atactgatat tgttgtgttg ttatcgtgtg
     1021 gattttaacc agggtttatc aggctgcgca actgcgtggc cttttttcat ttcttgggct
     1081 gtagtccccg tgtgtcattc aggcttccgg actacagccc actccatatc tgatttaata
     1141 cactatcccg gccgggagga ataatgacat ttaaacatta tgatgttgtc agggcggcgt
     1201 cgccgtcaga ccttgctgat gcacttgcgc aaaaaattcg tgaaggatgg caaccatatg
     1261 gtgggccgtt ttcttcgtat acggatgatg gcgcagcact tattcaggcg attgtcgcag
     1321 aaggtgatgt gagcacacct gttgtgg
//
LOCUS       DAZKHC020000329         1343 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZKHC020000329 DAZKHC020000000
VERSION     DAZKHC020000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.333"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1343
                     /locus_tag="ACHOSM_RS25860"
                     /old_locus_tag="pgaptmp_005172"
     CDS             <1..>1343
                     /locus_tag="ACHOSM_RS25860"
                     /old_locus_tag="pgaptmp_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4561437.1"
                     /translation="SPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYSGSQQNTGASDTTAQ
                     PGPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHDSDNETGETADLS
                     IKVNGHQEITSTSRTCNHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSK"
BASE COUNT          426 a          331 c          340 g          246 t
ORIGIN      
        1 tcagcccggt cattgctgcc attcatcgcg aatacaagca gacatggaaa acactggatg
       61 acgaactggc ctacgctctc tggcctggtg atgtggatgc cggaaacatt gacggcagca
      121 tccatcgctg ggcaaaaaat gaagttatcg acaacgaccg cgaagactgg aagcgtatct
      181 cggcatcaat gcgcaaacag cctgatgccc ttcgctacga ccgccagact atttttggcc
      241 ttgtccgtga acgtccgatc gacattcaca aagaccctgt ggcactgaac aaatacatta
      301 ctgaatacct gactacaaag ggcgtgtttg aagatgaagg aagaaatcag agcgcaactg
      361 atactctctc gtcgccagta ccagaaactg atgcagtgga aacggcaatt ccggacaacg
      421 aaaaaaccga atgcaaagtg gaagtcgaac catctgtaga gcgtgagggg ccgttctact
      481 tcctcttcac cgacaaggat ggcgaaaaat acggtcgcgc aaacaaactt tctggtctgg
      541 ataaggcact ggctgccggg gctactgaaa tcacgaaaga agaatatttc gcccgcaaaa
      601 acggtacata ctcaggttca caacaaaata ctggtgcatc tgacacgacc gcacaaccag
      661 ggccggtaaa agttaccgct gacgaagtaa acaaaattat gcaggcagcc aatatcagcc
      721 agcctgacgc cgataagttg cttgctgcct ctcgcggaga atttgttgca gggattagcg
      781 acccgaatga tccgaaatgg gttaagggga tccagacccg cgattctgta aaccagaacc
      841 agcatgaatc ggaacggaac taccaaaaag cggaacaaaa cagcccaaat gcgttacaaa
      901 acgagccaga aacgaaacag cctgaaccag tggcgcaaca ggaagtggaa aaagtctgca
      961 ccgcctgcgg tcagaccggc ggcggcaact gccctgattg tggcgcggtg atgggcgacg
     1021 caacatacca ggaaacattc gatgaagagt atcaggttga agttcaggaa gatgatccgg
     1081 aggaaatgga aggcgctgaa catccacaca aggagaacac tggcggcaat cagcatcacg
     1141 atagcgataa tgaaactggc gagacggcag atctctcaat taaggtgaac ggtcatcaag
     1201 aaatcacatc caccagcagg acgtgtaacc atctaatgat cgaccttgaa accatgggaa
     1261 aaaatcctga tgccccgatc atctcaatag gtgcaatatt tttcgatccg caaaccggag
     1321 atatgggacc ggaatttagt aag
//
LOCUS       DAZKHC020000330         1341 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZKHC020000330 DAZKHC020000000
VERSION     DAZKHC020000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.336"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            59..307
                     /locus_tag="ACHOSM_RS25865"
                     /old_locus_tag="pgaptmp_005173"
     CDS             59..307
                     /locus_tag="ACHOSM_RS25865"
                     /old_locus_tag="pgaptmp_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4561438.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            285..>1341
                     /locus_tag="ACHOSM_RS25870"
                     /old_locus_tag="pgaptmp_005174"
     CDS             285..>1341
                     /locus_tag="ACHOSM_RS25870"
                     /old_locus_tag="pgaptmp_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU4561439.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILL"
BASE COUNT          398 a          306 c          334 g          303 t
ORIGIN      
        1 accccggtcg ttgcccacca gctatagtgg cggcgaccat gattagcgaa cgacgctcat
       61 ggcaagactt attctgctca ctgagtgggc aaaagaggaa ttcagtgaac cggtccctac
      121 tccgagtacg ttaagtaaat acgctaaagc cggaatgata tttcctctcc ccaaaaaagt
      181 tggaagacgc tggcgagtgg atccgcaagc tcgctttgtc ggaatggtaa acaagccgga
      241 ggtgatcgcc acagatcacc ctgctttgaa gaggatactg gaagatggcg cgcccgcgaa
      301 aatataaaac cgatgttccg ggattatctc cgtattttga caaaagaaat aacaaagttt
      361 actggcgtta caggcatccc ataacaggca aaaatcacgg tctcggcagt attgaccaga
      421 aactggcaga aactattgca gcagaagcga acagccgtct tgcccggcag caaatggaac
      481 aaatgctcag tctgcaggag aaaattatta gtgataccgg cggttcatca accgttacca
      541 tttttctgaa taattacaga aaaattcaac aggaaagata tgaaaacggc gagatcaaac
      601 tcaacacgct gaaacagaaa gcggcccctc tcagggtatt tgatgaacgt tttggcacca
      661 gaccgttaga tgccataacc gtaaaagatg tggtatcagt actggaagag tacaaggcca
      721 gaggacataa cagaatggga caaattttca ggaaagtact gatcgatgtt ttccgggaag
      781 ctcagcaaac gggcgatgtc ccgccaggct ttaaccctgc agaatctgca aaaaaaccgc
      841 aggtgcggat atcaagacag cgactgactt ttgatgagtg gatgatgatt tataacgcag
      901 cggaaaagga tggttacttt ttacagcgcg gtatgctgct ggcactgatg acaggccagc
      961 gcctttcaga tatttgcaaa atgcaatttt cggatatccg ggatggttat cttcatgtcg
     1021 aacagcaaaa aacaggaacc cggattgcca tccctctggc tctgcgttgc gataaattaa
     1081 atctcaccct ggatgatgtg gtgtcatcct gccgcgattg cgttcttagt ccgtggctat
     1141 tgcaccacca tcacgcgaaa gggacagcta agcgcggcgg gatggttaag ccagcaacat
     1201 taaccgttgc atttaaaaaa gcccgggatt ctgtggatta caactggcgt gctaatggca
     1261 ccccaccctc tttccatgag cagagatctt tatcagagcg attgttcaga gagcaggggg
     1321 ttgataccaa aattttgcta g
//
LOCUS       DAZKHC020000331         1340 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZKHC020000331 DAZKHC020000000
VERSION     DAZKHC020000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.337"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..593
                     /locus_tag="ACHOSM_RS25875"
                     /old_locus_tag="pgaptmp_005175"
     CDS             <1..593
                     /locus_tag="ACHOSM_RS25875"
                     /old_locus_tag="pgaptmp_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4561440.1"
                     /translation="CDRVAALRKLPAEIKPAVMRIMASYAFEDYARSAASKKQCPCCH
                     GKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEWKKVREVVKVACPECGGKGE
                     VSTACKDCRGRGVAIHREESEKQGVPVFRNCQRCGGRGYERLPSTEAFNAICNVTDAI
                     SLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            820..1017
                     /locus_tag="ACHOSM_RS25880"
                     /old_locus_tag="pgaptmp_005176"
     CDS             820..1017
                     /locus_tag="ACHOSM_RS25880"
                     /old_locus_tag="pgaptmp_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4561441.1"
                     /translation="MLKQQDMTETARAVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            1168..>1340
                     /locus_tag="ACHOSM_RS25885"
                     /old_locus_tag="pgaptmp_005177"
     CDS             1168..>1340
                     /locus_tag="ACHOSM_RS25885"
                     /old_locus_tag="pgaptmp_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGU4561442.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWTGDEDYL"
BASE COUNT          361 a          266 c          372 g          341 t
ORIGIN      
        1 ggtgtgatcg cgtggcggcg ttaagaaaac tcccggcaga aataaaaccg gcagtgatgc
       61 gtattatggc ttcgtatgcg tttgaagatt atgcccgtag cgcggcgagc aaaaaacagt
      121 gcccctgttg tcacggaaaa aaatttattg aaagcgaggt ttttacaaac aagatccagt
      181 atccggatgg taagccgcca gtgtgggcaa agtgcacaaa aggcgtgtat ccgtcttact
      241 gggaggaatg gaaaaaagtc agggaggtgg taaaagttgc ctgtccggag tgtggaggga
      301 agggagaggt atccactgcc tgtaaagatt gtcgtgggcg cggtgttgcc attcatcgtg
      361 aagagtcgga aaaacagggt gtgccggttt tcagaaactg tcagcgttgt ggtgggcgtg
      421 gctatgaaag attaccttca acggaggcat ttaatgccat atgtaatgta accgatgcca
      481 tatctcttga tacatggaaa aaaacagtta aacgttttta cgatacgctg gtggtgcagt
      541 ttgatattga agaagcatgg gcagaacaac aactgaaaaa ggtgaccaga tagctttgtt
      601 gatttttccc gaatctgtgg taaatttgcc ctaacgatgg gcgttttatg cctgacgtta
      661 gaagattttt tacaccccgc cgcctggcgg gttttttatg actgaaatcg cgtcagtaca
      721 gtaaacgcgc tggtggcggt gaataccggt ctttcagctt gctggctttt ttgacaagag
      781 ttattggtgt gtcacgttaa ccgaaaaagg gaaaaagaca tgctgaaaca gcaggatatg
      841 acagaaactg ccagagcagt gtttaatgaa ttaagcgtca ccgaaccggc gacagtcggg
      901 gagattgcgc agaatactta cctttcacgc gaacgctgcc agttaatact gacccagctt
      961 gttatggcgg gtctggcaga ctatcagttc ggttgttaca gacgccttca gtcctgaagg
     1021 cttttttatt tgtggtaaat gggcgactgg tgggtgttag gggcactcac cagccatctg
     1081 ctcatgcgtc tggatcacaa gcaaacctca ggcccactgc tttgcgcaaa agcggtatga
     1141 gcctatcaga gacaggctta atgatccatg tttaatactg taaaaatatc cagttgtgag
     1201 ttaatcaacg ccgactgcct ggaatttatc cggtcgttac ccgaaaattc tgttgacctg
     1261 atagtcacgg acccgccgta ctttaaagtg aagcctgagg gctgggataa ccagtggacg
     1321 ggtgatgagg attacctgaa
//
LOCUS       DAZKHC020000332         1337 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZKHC020000332 DAZKHC020000000
VERSION     DAZKHC020000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.338"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1235
                     /gene="ygeH"
                     /locus_tag="ACHOSM_RS25890"
                     /old_locus_tag="pgaptmp_005178"
     CDS             <1..1235
                     /gene="ygeH"
                     /locus_tag="ACHOSM_RS25890"
                     /old_locus_tag="pgaptmp_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HGU4561443.1"
                     /translation="HVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIE
                     TIYRKGYRFSGQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKI
                     DGLYTYPMAATNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFL
                     IASNHLPVDELHNTSQFIIDNILQTVHKPERSVRLAKQDQGYNNHYLSDEMLAGKKEL
                     YDFTPESIYRAMTIFDRLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKAL
                     ELLDYVSDITTVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVH
                     FHNEKIEEARICIDKSLQLEPIRRKAVVIKECVDMYVPNPLKNNIKLYYRETESESHR
                     VIIDNILKLKQLTRICMR"
BASE COUNT          485 a          231 c          220 g          401 t
ORIGIN      
        1 gtcatgttgt actgaaagat atgatcatcg aatcagtatg gaaaaatatt attgttagtg
       61 acgagtccct gacaagatgt atctattctt tgcgctgcat ttttgaaaaa attggctatg
      121 atcgttgcat agaaacaatc taccggaaag gttatcgttt cagcgggcag gttttcaaaa
      181 ctaaaattaa tgaagataat acttcagact attccatagc tatattccct ttcactactt
      241 cattgaatac actggatcca ttaatactta atcaggaatt agtgcaaatc atttcaaata
      301 aaaaaatcga tggtctctat acctatccga tggctgcgac aaatttttgt aatgatcaca
      361 tatctcaaaa ttcattcttg agcagattca agccagatta tttcgttaca ggaagaataa
      421 accagaataa tgcagtgaac actttataca ttgagttgat cgacgctaaa aaccttttcc
      481 tcatcgccag taatcatctc cctgttgatg aactacataa tacatcacaa tttattatag
      541 ataatatcct tcaaacggta cataaaccag aacgatctgt aagattagct aagcaggacc
      601 aaggatataa taatcattat ttatcagatg aaatgttagc cggaaagaaa gaactttacg
      661 acttcacccc tgaaagcatt tacagggcca tgactatatt tgatagatta caaaataaaa
      721 gtgatataca gacgctaaaa acagaatgtt attgccttct agcggaatgc catatgtctt
      781 tggcacttca tggaaaaagt gaacttgaac ttgctgctca aaaagcatta gagcttttag
      841 attatgtatc agacataacc actgtcgatg gaaaaatttt agctattatg ggactgataa
      901 ctggtctgtc tggacaagca aaagtatctc atatcttatt tgaacaggct aagatacact
      961 caactgatat agcctctctc tactactata gggcactagt ccactttcat aatgaaaaaa
     1021 ttgaagaggc aaggatttgt atagacaaat cactacaact cgaacccata agacgaaaag
     1081 cagttgtgat aaaagaatgt gtagatatgt atgtgcctaa cccgctcaaa aacaacatta
     1141 aactctacta tagagaaact gagagtgaaa gccatcgagt tataattgac aacattttga
     1201 aattaaagca gctgacgaga atttgtatgc gataaatttt tattgacgca attttattat
     1261 ttagatgtat gcaacttgtt atttaaacaa cagagataaa aatatctaag atattcacct
     1321 tattgcaaga tatttaa
//
LOCUS       DAZKHC020000333         1333 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZKHC020000333 DAZKHC020000000
VERSION     DAZKHC020000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.339"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(459..1034)
                     /locus_tag="ACHOSM_RS25895"
                     /old_locus_tag="pgaptmp_005179"
     CDS             complement(459..1034)
                     /locus_tag="ACHOSM_RS25895"
                     /old_locus_tag="pgaptmp_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGU4561444.1"
                     /translation="MPLTSAIASNSFSTGMQVLRAQMAASGGGEITVGGQTVSITYSE
                     TDGRFLASGGNNSLLSGLLLTGLNGGPEALRDIMLRMVSGSGNTQSHGDIEGKISQCK
                     FSVNTESLQCPSEAVRCPIILDKPEEGVFVKNSEGSLVCTLFDSVSFSHLVRDGGKHP
                     LTREPITSSMIVSQEQCIYDQTKGNFVIKDK"
     gene            complement(1147..>1333)
                     /locus_tag="ACHOSM_RS25900"
                     /old_locus_tag="pgaptmp_005180"
     CDS             complement(1147..>1333)
                     /locus_tag="ACHOSM_RS25900"
                     /old_locus_tag="pgaptmp_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4561445.1"
                     /translation="QLRLGPADILESDENGIIPEQDRVITQVVILDADKKQIQCVVRP
                     LQILRADGTWENIGGMK"
BASE COUNT          364 a          371 c          228 g          370 t
ORIGIN      
        1 cccttcactt tctgctaaaa aacggcctgt atctggcata taagaaactc taactctctc
       61 ccctcccagg agagatccac caatctgccc ttccattacc tgctgacgca tctccgccat
      121 ctcattcgcg aatgaagtcg atgatatata tgatactaat ccaggcatta ccatactcct
      181 tcatacagat aaatatttcc tctgggcttc ataacaaaca tctccctgac atgacaacaa
      241 aaaccggagc cggactccgg tttttgtgaa tccgtcggct atttcatccc gccaatattt
      301 ccccgtcagc acgccagatt tgcagcggcc tcaccactca ctgtatatgc tttttagctg
      361 catccagtac accaattacc acatccttat accctgaaca tctgtagcat ttcatataag
      421 caactggcag ttaacatata gcttgggtaa tatttatttc acttatcctt tatgacaaag
      481 tttcctttgg tttggtcata aatacattgt tcttgactta caatcattga tgacgttatt
      541 ggttctcgtg ttagcgggtg cttcccaccg tcgcgaacca aatgagaaaa agaaaccgaa
      601 tcaaataagg tacaaaccaa agaaccttct gaatttttaa caaacacacc ttcttctggt
      661 ttatccagta taattgggca tcgaaccgcc tcggatggac actgaaggct ctccgtatta
      721 acagaaaact tacattgtga tattttcccc tcaatatcac catgtgattg tgtgttacct
      781 gaacctgaaa ccattcttaa cattatatcc ctgagcgctt caggaccacc attaagccct
      841 gtaagtaata atccagaaag caaactgtta ttgcccccac tcgccagaaa gcgaccatcc
      901 gtttcactat atgtgatact gaccgtctgc ccgcctactg taatttctcc accgccactg
      961 gcggccattt gagcacgaag aacctgcatt ccggtggaaa atgaattgga tgcaatagct
     1021 gatgttaacg gcatgacaaa actccctatt taaatattca ttccaaacac agggagtcat
     1081 aacaaacagc ccccatacat gacaacaaaa accggagccg gactccggtt ttgtgaagct
     1141 gtcgggttac ttcatcccgc caatattttc ccacgtcccg tcagcacgca ggatttgcag
     1201 cggtcttacc acgcactgta tctgcttttt atccgcatcc agtatcacca cctgcgtgat
     1261 taccctgtcc tgctccggga taatgccatt ctcatctgac tccagaatgt ctgccggtcc
     1321 cagtcgcagc tgt
//
LOCUS       DAZKHC020000334         1325 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZKHC020000334 DAZKHC020000000
VERSION     DAZKHC020000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.340"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..927
                     /locus_tag="ACHOSM_RS25905"
                     /old_locus_tag="pgaptmp_005181"
                     /pseudo
     CDS             1..927
                     /locus_tag="ACHOSM_RS25905"
                     /old_locus_tag="pgaptmp_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            901..>1325
                     /locus_tag="ACHOSM_RS25910"
                     /old_locus_tag="pgaptmp_005182"
     CDS             901..>1325
                     /locus_tag="ACHOSM_RS25910"
                     /old_locus_tag="pgaptmp_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4561446.1"
                     /translation="MNGIDPLGLSPADVALIRRKDQLNHQRAWDILSDTYEDMKRLNL
                     GGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNLFGMYGRKVKLSHSEM
                     IEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKKTI"
BASE COUNT          362 a          292 c          419 g          252 t
ORIGIN      
        1 ctatgatgaa tacggcagac tgacggagaa gacggaccgc atcccgacgg gtgtgatacg
       61 gacggacgac gagcggaccc accactacca ctacgacagc cagcaccgcc tggtgttcca
      121 cacgcggata cagcatggcg agccactggt cgagagccgc tacctctacg acccgctggg
      181 acggcgaatg gcgaaacggg tatggcggcg ggagcgtgac ctgacggggt ggatgtcgct
      241 gtcgcgtaaa ccggaggaga catggtacga ctgggacggc gacaggctga cgacggtaca
      301 gaccgacacc acgcgtatcc agacggtata cgagccggga agcttcacgc cgctcatccg
      361 ggtcgagaca gagaacggcg agcgggagaa agcgcagcgc cgcagcctgg cggagaagct
      421 gcagcaggaa gggagcgagg acgggcacgg cgtggtattt ccggctgaac tggtgcggct
      481 gctggacaga ctggaggaag aaatccgggc agaccgcgtg agcagtgaaa gccgggtgtg
      541 gcttgcgcag tgcgggctga cggtggagcg gctggcggca cagatagagc cggtttattt
      601 gcctgagcga aaaatacatc tttatcactg tgaccaccgg ggcctgccgc tggcgcttat
      661 cagtgaagac ggcaatacgg cgtggagcgc ggaatatgat gaatgggcaa ccagcttaat
      721 gaggagaacc cgcatcacct gcaccagccg taccggctgc cggggcagca gtatgataag
      781 gagtcggggc tgtactacaa ccggaaccgg tactacgatc cgttgcaggg gcggtatatc
      841 actcaggacc cgatagggct ggagggggga tggagtctgt atgcgtatcc gctgaatccg
      901 gtgaatggta ttgatccatt agggttaagt cccgcagatg tagcgctaat aagaagaaaa
      961 gatcaactaa accatcaaag agcatgggat atattatctg atacttatga agatatgaag
     1021 agattaaatt taggtgggac tgatcaattt ttccattgta tggcattttg tcgagtgtct
     1081 aaattaaatg acgctggtgt tagccgatcg gcgaaagggc tgggttatga aaaagagatt
     1141 agagattacg ggttaaatct gttcggtatg tacggcagaa aagtaaagct atcccattct
     1201 gaaatgattg aagataataa aaaagacttg gctgtaaatg accatgggtt gacatgtcca
     1261 tcaacaacag attgctcaga tagatgtagt gattatatta atccagagca taaaaaaacg
     1321 ataaa
//
LOCUS       DAZKHC020000335         1274 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZKHC020000335 DAZKHC020000000
VERSION     DAZKHC020000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.341"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..99
                     /locus_tag="ACHOSM_RS25915"
                     /old_locus_tag="pgaptmp_005183"
     CDS             <1..99
                     /locus_tag="ACHOSM_RS25915"
                     /old_locus_tag="pgaptmp_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4561447.1"
                     /translation="LFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
     gene            101..370
                     /locus_tag="ACHOSM_RS25920"
                     /old_locus_tag="pgaptmp_005184"
     CDS             101..370
                     /locus_tag="ACHOSM_RS25920"
                     /old_locus_tag="pgaptmp_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4561448.1"
                     /translation="MNILKKLMQRLCGYGKHDDREHGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            688..837
                     /locus_tag="ACHOSM_RS25925"
                     /old_locus_tag="pgaptmp_005185"
                     /pseudo
     CDS             688..837
                     /locus_tag="ACHOSM_RS25925"
                     /old_locus_tag="pgaptmp_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1058..1195
                     /locus_tag="ACHOSM_RS25930"
                     /old_locus_tag="pgaptmp_005186"
                     /pseudo
     CDS             1058..1195
                     /locus_tag="ACHOSM_RS25930"
                     /old_locus_tag="pgaptmp_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
BASE COUNT          355 a          211 c          387 g          321 t
ORIGIN      
        1 ctgtttcagc atttgcaggt caggtttggt gacgggccgt ggcaggatgt taaggggctg
       61 gatgaagtgg gcagtgatac aggcagaaca ggagaatgac atgaacatac taaaaaaact
      121 tatgcagcgt ctgtgcgggt acggaaagca tgatgaccgt gaacacgggg agttacttac
      181 agcacaactg cgtctgggac cggcagacat tctggagtca gatgagaatg gcattattcc
      241 ggagcaggac agggtaatca cacaggtggt gatactggat gcggataaaa agcagataca
      301 gtgcgtggta agaccgctgc aaatcctgcg tgctgacggg acgtgggaaa atattggcgg
      361 gatgaaatag ccgacgggtt cacaaaaaac cggaggccgg ctccggtttt tgttgtcatg
      421 tatgggggat gtttgttatt agagtgtgaa gtaataaaca tgttaatacg atggagtgaa
      481 ggatgccttt aaggagcagg ggagtgtcca tcttgttccc tggaggggga atcataaatt
      541 ctgattgcga ccgaggcatg gaacactact gtagttcagg gaagtagggc gatgaagctc
      601 attcttgcga cgcgtaatta ttatctggaa tatggtttgc gtttgttact gaaaggatgc
      661 cgtgtaattc tggctcaaga attttttatg ccggaaaatc gcagggttat tctggatagt
      721 aaagaatcct ggttaataat ctgtgatagt cagttgggcc atttaatgcg cagcatgttt
      781 cagggacgcc gttttattca gctggatctg gaagcgttga aagggggaca tgatatacat
      841 aatgctgtgc gtaacagatt gtggacctgg aacaaaaaag cacgggcact gacgatgtca
      901 gagatggtgg tgatgtttgg atatatctac cgtcagttgc gtccgtccca tctcgccagt
      961 gagatgaggg taaacataaa aacagttaac actttcctgt attcagggct ggcgaaaaat
     1021 gggctcaaaa gaagtagtgt aaggttgctg gcaatttctg agaacaagcg aatagggcat
     1081 tattccggag caggacaggg taatcacgca ggtggtgata ctgggtgcgg ataaaaagcc
     1141 gatacagtgc gtggtgaggc cgctgcaaat tcggtgtgct gatgggacga gggaaaaatt
     1201 tgtggatgac atttgttgtg gacctttaag tctggagttc aaattaaaac agggagcttt
     1261 attatgccat taac
//
LOCUS       DAZKHC020000336         1271 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZKHC020000336 DAZKHC020000000
VERSION     DAZKHC020000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.342"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1248
                     /locus_tag="ACHOSM_RS25935"
                     /old_locus_tag="pgaptmp_005187"
     rRNA            <1..1248
                     /locus_tag="ACHOSM_RS25935"
                     /old_locus_tag="pgaptmp_005187"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          320 a          294 c          397 g          260 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaaccttac ctggtcttga catccacgga agttttcaga
      721 gatgagaatg tgccttcggg aaccgtgaga caggtgctgc atggctgtcg tcagctcgtg
      781 ttgtgaaatg ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg ttgccagcgg
      841 tccggccggg aactcaaagg agactgccag tgataaactg gaggaaggtg gggatgacgt
      901 caagtcatca tggcccttac gaccagggct acacacgtgc tacaatggcg catacaaaga
      961 gaagcgacct cgcgagagca agcggacctc ataaagtgcg tcgtagtccg gattggagtc
     1021 tgcaactcga ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat gccacggtga
     1081 atacgttccc gggccttgta cacaccgccc gtcacaccat gggagtgggt tgcaaaagaa
     1141 gtaggtagct taaccttcgg gagggcgctt accactttgt gattcatgac tggggtgaag
     1201 tcgtaacaag gtaaccgtag gggaacctgc ggttggatca cctccttacc ttaaagaagc
     1261 gttctttgaa g
//
LOCUS       DAZKHC020000337         1251 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZKHC020000337 DAZKHC020000000
VERSION     DAZKHC020000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.343"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..212
                     /locus_tag="ACHOSM_RS25940"
                     /old_locus_tag="pgaptmp_005188"
     CDS             <1..212
                     /locus_tag="ACHOSM_RS25940"
                     /old_locus_tag="pgaptmp_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGU4561449.1"
                     /translation="CPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRI
                     RSNNCYGQVSRRDQESALACWGIDR"
     gene            510..1004
                     /locus_tag="ACHOSM_RS25945"
                     /old_locus_tag="pgaptmp_005189"
     CDS             510..1004
                     /locus_tag="ACHOSM_RS25945"
                     /old_locus_tag="pgaptmp_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4561450.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNVITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGT
                     VREATGTSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAQDYIRTQCTKQAFYY
                     PEDV"
     gene            1001..1225
                     /locus_tag="ACHOSM_RS25950"
                     /old_locus_tag="pgaptmp_005190"
     CDS             1001..1225
                     /locus_tag="ACHOSM_RS25950"
                     /old_locus_tag="pgaptmp_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561451.1"
                     /translation="MKKLQVTVKPFQGTIPFRVLQHGRVLLEEVFRGKCTECYSRTYE
                     VNATHEEFTVECVMNTDKCRMVSAELQPVC"
BASE COUNT          321 a          274 c          353 g          303 t
ORIGIN      
        1 tctgtccgta caacattggc cccggtaagt gtttcccgtc gacgttttac agacggatta
       61 atgctggtga ccgcagggga gcatgcgagg cgattcgctg gtggattaag gacggtggca
      121 gagattgccg tattcgctca aacaactgct acggtcaggt ctcacggcgt gaccaggaga
      181 gcgcgctggc gtgctgggga attgacagat aagaagaata ttttgctgaa aaatgaggtt
      241 tgctcgcacg ggcggataac acgaaatcct gcgaactggc aaaaactaag tgaataaaag
      301 taaaaccccg tttgttggcc gcaagcgggg ttttgtgttt cctgactccg gaaaagtcaa
      361 aggagaaagt gtgtttgatt ttagcaaact gattcgggag attcgaatga tggctgaaaa
      421 attatccacc tggaagttca tccttatctg gctggtgttt gtgattatgg cttccggtta
      481 tttcattggt cagatacgct ggtggtgaaa tgaaccgcgt actgtgcgtg gtcatcattg
      541 ccctgctggt ggcctgtggt gcgcttagtc tggggctgaa tcattaccgc gataacgtca
      601 tcacctacaa agcgcagcgc gataaaaaag ccagagagct ggagctggca aacgcaacca
      661 ttactgatat gcaggtgcgc cagcgcgatg ttgctgcgct cgatgcaaaa tactcgaggg
      721 aattagccga tgcgagagct gaaaatgaaa ctctgcgcgc tgatgttgcc gctggtcgta
      781 agcgcctgcg gatcaacgcc acctgccccg gtaccgtgcg tgaagccacc ggcacctccg
      841 gcgtggataa tgcaaccggc ccccgactgg cagacaccgc tgaacgggat tatttcatcc
      901 tcagagaacg gttgatgaca atgcagaagc agctggaagg ggcacaggac tatatccgca
      961 ctcagtgtac taagcaggct ttttattatc cggaggatgt atgaagaaat tacaggtaac
     1021 ggtaaaacct tttcagggaa caattccgtt ccgtgttttg caacatggcc gtgttctgct
     1081 tgaagaggtg ttcagaggta aatgcactga atgttattca cgaacatatg aagtgaatgc
     1141 cacgcatgaa gaattcaccg ttgagtgtgt gatgaatact gataaatgcc gaatggtatc
     1201 cgctgaatta cagccagtgt gttgagcgac cttattatcc atgcgcggta t
//
LOCUS       DAZKHC020000338         1239 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZKHC020000338 DAZKHC020000000
VERSION     DAZKHC020000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.344"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..155)
                     /locus_tag="ACHOSM_RS25955"
                     /old_locus_tag="pgaptmp_005191"
     CDS             complement(<1..155)
                     /locus_tag="ACHOSM_RS25955"
                     /old_locus_tag="pgaptmp_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGU4561452.1"
                     /translation="MNIEELKTKTEADISEYITKKIIELKKKTGKEVTSIQFTAREKM
                     TGLESYD"
     gene            complement(328..558)
                     /gene="ynfN"
                     /locus_tag="ACHOSM_RS25960"
                     /old_locus_tag="pgaptmp_005192"
     CDS             complement(328..558)
                     /gene="ynfN"
                     /locus_tag="ACHOSM_RS25960"
                     /old_locus_tag="pgaptmp_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HGU4561453.1"
                     /translation="MKKIIVFFNSEPAMVVPVMTGVNTIMREYPNGETTHVTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAMRLLN"
     gene            complement(590..628)
                     /locus_tag="ACHOSM_RS25965"
                     /old_locus_tag="pgaptmp_005193"
                     /pseudo
     CDS             complement(590..628)
                     /locus_tag="ACHOSM_RS25965"
                     /old_locus_tag="pgaptmp_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(631..819)
                     /gene="ynfQ"
                     /locus_tag="ACHOSM_RS25970"
                     /old_locus_tag="pgaptmp_005194"
     CDS             complement(631..819)
                     /gene="ynfQ"
                     /locus_tag="ACHOSM_RS25970"
                     /old_locus_tag="pgaptmp_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGU4561454.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDRNPFGAKCIFCKSMMITF
                     DNISQYLNVSRLSLDLRK"
     gene            complement(830..1042)
                     /gene="cspI"
                     /locus_tag="ACHOSM_RS25975"
                     /old_locus_tag="pgaptmp_005195"
     CDS             complement(830..1042)
                     /gene="cspI"
                     /locus_tag="ACHOSM_RS25975"
                     /old_locus_tag="pgaptmp_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HGU4561455.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQQVEFGIENGPKGPAAVHVVAL"
BASE COUNT          402 a          243 c          203 g          391 t
ORIGIN      
        1 atatcatagc tttcaagacc agtcattttt tcccgtgcag taaactggat actggtaact
       61 tctttcccgg tctttttctt aagctcaata attttttttg ttatatattc agaaatatct
      121 gcttctgttt ttgtttttaa ctcttcaatg ttcatcattt cctcttttag tctgttatga
      181 ctttcctggt acacagtagt gtcaattata tggagcaaac gtataaaaga taagatgaaa
      241 catcgcaata atcaacatac gacagtctaa atttcgcaca aacagataaa gatgattatc
      301 ctttattatc aaggcattag aatgaaatca attcaagagt ctcattgctg cttccagaat
      361 ttcttctgaa gttacatgtc gatccgctgc tacataaatg actttatgat ctccggtcag
      421 agatggaaac cctgcagcca ttacagtgac atgtgttgtt tcgccatttg gatattcacg
      481 catgatggtg ttaactccgg tcatcactgg cactaccatt gctggttcag agttaaaaaa
      541 aactattatt tttttcatga tgttaccgta atatgtgagt atccatcgac tagacactaa
      601 gcaaaaaagc tcccgaagga gccttcattt tcactttctt aaatctaacg acagacggct
      661 aacatttaag tattgtgaaa tattatcaaa tgtaatcatc attgatttac aaaagataca
      721 ttttgccccg aaaggattcc tgtcagaaac atcaaaagat gatgttctat actgagaacc
      781 atggcaacac gggcatctaa agtgaatatg gtttgtaata ttttctacct caaagtgcca
      841 ctacatgaac agccgcgggg cctttaggtc cgttctcaat accaaattcg acttgctgat
      901 tctcagttaa cgttttgaaa tcgttgctct gaatagctga gaaatggaca aacacatctt
      961 tgctaccatc tttcggcgtg atgaaaccaa aacctttttc agggttaaac catttcacta
     1021 aaccagtcat tttgttagac atcattatta ccttttgaag aagtgagccc ttgggcagaa
     1081 tggtccgaaa aaaatatcag agagaaaaac caacaaggaa atctcaagag gtacaaataa
     1141 taaaattata acaatgactg cttcagataa ttttgtaaca aaccagaaca ccattaacgc
     1201 atgattaacc aaccatagca aggattactt ttgtaaaga
//
LOCUS       DAZKHC020000339         1236 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZKHC020000339 DAZKHC020000000
VERSION     DAZKHC020000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.345"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            50..319
                     /locus_tag="ACHOSM_RS25980"
                     /old_locus_tag="pgaptmp_005196"
     CDS             50..319
                     /locus_tag="ACHOSM_RS25980"
                     /old_locus_tag="pgaptmp_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4561456.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDTDKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            425..>1236
                     /gene="nleH"
                     /locus_tag="ACHOSM_RS25985"
                     /old_locus_tag="pgaptmp_005197"
     CDS             425..>1236
                     /gene="nleH"
                     /locus_tag="ACHOSM_RS25985"
                     /old_locus_tag="pgaptmp_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase NleH"
                     /protein_id="HGU4561457.1"
                     /translation="MLSPSSVNLGCSWNSLTRNLTSPDSRILSSVRDAAASSDNGAQV
                     KVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRAM
                     ERGAVHSNRPDLPPVDYAPPELPSVDYNSLPVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTNEVRCFNQYYGAGSAEKIYGDNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFIDTTETNVLYDRTRNEFNPIDISSYNISERSWS"
BASE COUNT          373 a          205 c          325 g          333 t
ORIGIN      
        1 aaggggctgg atgaagtggg cagtgataca ggcagaacag gagaatgaca tgaatatact
       61 aaaaaaactt atgcagcgtc tgtgtggttg cggaaagcat gatggccgtg aacacgtgca
      121 gtcgcttaca gcacaactgc gactggggcc ggcagacatc ctggagtcag atgagaatgg
      181 cattattccg gagcaggaca gggtaatcac acaggtggtg atactggata cagataaaaa
      241 gcagatacag tgtgtggtaa gaccgctgca aatcctgcgt gctgacggga cgtgggaaaa
      301 tattggcggg atgaagtaac ccgacagctt cacaaaaccg gagtccggct ccggtttttg
      361 ttgtcatgtc atggtgatgt ttgttaagaa agtaaagatt gattcatttt gaaggttgaa
      421 atgtatgcta tcaccatctt ctgtaaattt ggggtgttca tggaattctt taaccagaaa
      481 cctgacttct cctgacagtc gtattttatc ctctgtaagg gatgcagctg ctagctcgga
      541 taatggggcg caagtaaagg tgggcaacag aacatatcgt gttgttgtca ctgataataa
      601 gttttgcgtt acaagagaaa gtcatagtgg ttgctttact aatctgttgc acaggctggg
      661 atggcctaag ggagagatta gcaggaaaat tgaggtgatg ctgaattcat cgccagtgaa
      721 tagggctatg gaaagaggtg ctgttcattc gaatagacct gatttacctc ctgttgatta
      781 tgcaccgcca gagttaccga gtgtggacta taacagcttg cctgtgcctg gtaatgttat
      841 tggcaaaggg ggtaacgctg tagtatatga agatgctgag gatgcaacaa aagtcctgaa
      901 gatgtttact acatctcaaa gcaatgaaga ggtaacaaac gaagttcgtt gcttcaacca
      961 atattatggt gccgggagtg cagaaaaaat atatggcgat aatggcgata ttattggtat
     1021 tagaatggat aaaataaatg gagaatcgct tttaaatatt tcgtccttgc cggcacaggc
     1081 tgagcatgcc atttatgata tgtttgacag gcttgagcaa aaaggaattc tttttatcga
     1141 tacaacagag acaaatgtct tatatgatcg cacgagaaat gaatttaatc caatagatat
     1201 atcatcttat aatatttctg aacgttcatg gagtga
//
LOCUS       DAZKHC020000340         1224 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZKHC020000340 DAZKHC020000000
VERSION     DAZKHC020000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.346"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..374)
                     /locus_tag="ACHOSM_RS25990"
                     /old_locus_tag="pgaptmp_005198"
     CDS             complement(<1..374)
                     /locus_tag="ACHOSM_RS25990"
                     /old_locus_tag="pgaptmp_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4561458.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATRDPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGVA"
     gene            618..941
                     /locus_tag="ACHOSM_RS25995"
                     /old_locus_tag="pgaptmp_005199"
     CDS             618..941
                     /locus_tag="ACHOSM_RS25995"
                     /old_locus_tag="pgaptmp_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU4561459.1"
                     /translation="MNWIVIDTVIQPACGISFSAIWCNIKLILWYQSDTFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVAPFNKNVWNLIKNSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
BASE COUNT          363 a          331 c          245 g          285 t
ORIGIN      
        1 caggcgacac cacccggtgt caccgtactg cggcgcgcca gctgcttaat acgcgggtcc
       61 ggacggtcat atgtctccgg cagcggaagg ccttcaccat atgccatgct gtttgactgc
      121 cccgccagaa ccacaacaaa gtaatactcc gggtctctgg tggcactgac agccaccgcc
      181 ttaccctcat taccggtcac cgccactggt gtggtgacat caccttccgc cgcaatggcc
      241 tgcatcaggg tataaggcgt gatggccacc ggactgccaa atggctgcca gccctccttc
      301 agtttttgtg tcagtcgttc agcaaggtct gacggcgatg ccgccctgac cacatcgtag
      361 tgtttaaatg tcataacctc tcctgccgcc agcaaagtaa ttatccggaa cacacaatca
      421 tgtaacacat aaaaaacacg ctataaaaaa ccatgactct tatataacaa aaaaaacgcc
      481 gacaatttaa aatgccctct tattttaaac aaagaataaa cgacaccaga aaaacaactc
      541 agcctgacag attgctttca aaacatcacc tataatttat ggcatcattt aaaaccttaa
      601 gaaaataagg aaagcaaatg aactggatag tgattgacac ggtaatccag ccagcatgtg
      661 gcatatcatt ttcagccata tggtgtaaca taaaattaat attatggtat cagtcagata
      721 ccttcctccc tccgggctgt atttttacac caacccggac aggcgtaata ctcaataata
      781 aagagctccc cgtcaccatt tacaacgtgg ccccatttaa taaaaacgtc tggaatttaa
      841 ttaaaaacag ccaggagtgc cctgcaaaca caggaaatat cacagataag tgttttaatc
      901 atcgctgcat tctggaaata tgcccatacg ggcgaaaata acagaatcat tcagataaaa
      961 agcccctccg gagaggggct gacactgcgt atctgtatca tcatgtacat ggtgccgggt
     1021 gcctcccggt gagttcagtc cggtgtccct gaacccgcgt atctcgatcc aggttgtcgt
     1081 cagagatgac accttataca ccagtcgccc ctccgcacag ggggattcac catgcgaatt
     1141 tttttaacaa atgctcagtc tgacaggcaa ctgtcaactg actgaattgt gacacagatt
     1201 acacttgtta cccacatacc acga
//
LOCUS       DAZKHC020000341         1212 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZKHC020000341 DAZKHC020000000
VERSION     DAZKHC020000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.347"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1032)
                     /locus_tag="ACHOSM_RS26000"
                     /old_locus_tag="pgaptmp_005200"
     CDS             complement(<1..1032)
                     /locus_tag="ACHOSM_RS26000"
                     /old_locus_tag="pgaptmp_005200"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGU4561460.1"
                     /translation="MIKKISINFLFLMLMIDVVFATLFNIPVWMHLFNIINNLDGVKL
                     GFIISLPVFLISALNFVFTPFSFRYIFKPFFCILFICSSIVTYATMKYGVQFDKTMMQ
                     NIFETNAGEMTSYFNMSVVLWFLFTGILPCGLLLLVNIRYPETWIKGIIYRLISMFAS
                     LLIIFVIAFFFYKDYASVGRNNSSLNKEIIPTNYIYSGFKYVRDFFVSPGEFRQTGTD
                     ASRTINEKQKPVIMFLVVGETARSQNYALNGYSRGTNDFTKKYNELISFHNVQSCGTS
                     TAISVPCMFSDMKRKEFNSRKAVNSENVLDILYRTGVNLLWIENDGGCKGVCKRIPTI
                     NIEPSNSDNT"
BASE COUNT          440 a          221 c          172 g          379 t
ORIGIN      
        1 tgtattatct gaatttgatg gctcaatatt aattgtaggg attcgcttac atacaccttt
       61 acatcccccg tcattttcaa tccagagtaa atttaccccc gttctgtaca gaatatccag
      121 tacattttcc gaatttaccg ctttgcggga attgaactct tttcgcttca tatctgaaaa
      181 catgcatgga acagatatgg ctgtggatgt gccacacgac tgcacattat gaaaggatat
      241 aagttcatta tatttctttg taaaatcatt ggtaccacga gaatatccat tcagagcata
      301 gttttgactt cttgctgtct ccccgacaac taaaaacata attaccggct tttgtttttc
      361 atttattgtg cgggatgcat ccgttccagt ctgacgaaac tcgccaggag aaacaaaaaa
      421 atcccttaca tatttaaacc cactgtagat ataattagta ggtattattt ctttatttaa
      481 tgaagagttg tttctgccaa ctgatgcata atccttataa aagaaaaatg caatcacaaa
      541 tataatcaag agagatgcaa acattgatat taaacgatat ataataccct ttatccatgt
      601 ctcaggataa cggatattta ctaacaacaa tagtccacat ggaagtatac ccgtaaataa
      661 aaaccacagt acaacagaca tattgaaata tgatgtcatt tcacctgcat ttgtttcaaa
      721 aatattctgc atcattgttt tatcaaactg cacaccatat ttcatggtgg catatgtaac
      781 aatggaacta cagataaaca gaatacaaaa aaaaggttta aatatatacc tgaatgagaa
      841 cggtgtaaaa acaaaattta atgcagatat cagaaagact ggaagagata taataaatcc
      901 aagtttcaca ccatcaaggt tattaataat attaaacaga tgcatccaga cagggatatt
      961 gaacaatgtg gcaaaaacaa catctatcat taacatcaag aaaagaaaat taatactgat
     1021 ttttttaatc atcttacccc cattaattag agcatataat tgtccgtatc taaaagtgat
     1081 atatttttat aaaacctggt aaagtgaata ctgatttata ccaggtcaaa cttcaacttc
     1141 atcatcaaaa ctttatgtca ataaaggcgt ttatagttac tgattctttt attttacaac
     1201 accactaaca tc
//
LOCUS       DAZKHC020000342         1210 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZKHC020000342 DAZKHC020000000
VERSION     DAZKHC020000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.348"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            17..>1210
                     /locus_tag="ACHOSM_RS26005"
                     /old_locus_tag="pgaptmp_005201"
     CDS             17..>1210
                     /locus_tag="ACHOSM_RS26005"
                     /old_locus_tag="pgaptmp_005201"
                     /inference="COORDINATES: protein motif:HMM:TIGR01586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HGU4561461.1"
                     /translation="MSLSALSEGLTGNRSLIDCISSIVHNKLPSVSEILLKEREYRLP
                     SIYSIMDSSLVDPVTWESIGYTGSDSYIKSPISSPTLHDISIRAKYRALEWGNFYGHN
                     ARLWYETAINYSSSEPRYHPQMLLSPNEGRCIGLSELYILADTKEKYNTLQENLDLIS
                     SLYQQDISDNAHLSESDHRLLKNAVSQIEYYQQHGNNKLLQSEALERIRLTDFNTASV
                     AHYLKDKNINNILITTEHHSLVVSVFDDVVRVTDPNFGYADFSSLEQSLEFIERSVII
                     SPDIKEIYTGKKGGVAIDMFIMEDNQWGKIIDHDACQLTSVKHLSSVEKLRKLNMDIQ
                     LGDGVFKIIDLYNYGVFFNGERIDEKFSMSKLTFNELFRMTISLDILKQYIDTHHITA
                     KEHEKI"
BASE COUNT          435 a          189 c          211 g          375 t
ORIGIN      
        1 aaagcaatat gaacatatgt ctttaagtgc tcttagtgaa ggattaacgg gcaacagatc
       61 tcttatcgac tgcatttcat ctatcgttca taacaaactg cccagcgtat cagaaatcct
      121 cttgaaggaa agagaatata gacttccatc aatatattct ataatggatt catcattagt
      181 tgatcctgta acatgggaaa gtataggcta tacaggaagc gatagttata ttaaatcacc
      241 aatatcatct ccgacattac atgatatttc tattcgggca aaatatcgag ctctggaatg
      301 gggaaacttt tatgggcaca atgctagatt atggtatgaa accgcgataa actactccag
      361 tagtgagcca agataccatc ctcagatgtt gctttccccc aatgaagggc gttgtatcgg
      421 tttaagtgag ttatatatcc ttgctgatac taaagaaaaa tataatacgt tacaagagaa
      481 ccttgattta atcagctcat tatatcaaca agatatatct gacaatgcac atctttcaga
      541 aagtgatcat cgactgttga aaaacgcagt atcacaaatt gaatattatc aacagcatgg
      601 taataataaa ttattacagt ccgaggcgct tgaaagaata cggttaactg attttaatac
      661 agccagtgtt gctcattatt taaaagacaa aaacataaat aatattctga taaccacaga
      721 acatcacagt cttgtagtat ccgtcttcga tgatgtagtt cgtgttacag atcctaactt
      781 cggatatgct gatttttcat ctctggaaca atcattagaa tttattgagc gttctgttat
      841 catttctcca gatataaaag aaatctatac gggtaagaaa ggtggtgtcg ctatagatat
      901 gtttatcatg gaagataatc aatggggaaa aattattgat catgatgcct gtcaacttac
      961 atcagtaaaa catttatctt cagttgaaaa attgagaaag ttgaatatgg atattcaact
     1021 tggtgatgga gtatttaaaa ttatcgattt gtataattat ggcgttttct ttaatggtga
     1081 aagaatagat gaaaaattta gtatgtcaaa gcttacattt aatgaattat tcagaatgac
     1141 aataagctta gatatattga aacaatatat tgatacgcac catataacag caaaagaaca
     1201 tgaaaaaata
//
LOCUS       DAZKHC020000343         1194 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZKHC020000343 DAZKHC020000000
VERSION     DAZKHC020000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.349"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..899
                     /gene="tssI"
                     /locus_tag="ACHOSM_RS26010"
                     /old_locus_tag="pgaptmp_005202"
     CDS             <1..899
                     /gene="tssI"
                     /locus_tag="ACHOSM_RS26010"
                     /old_locus_tag="pgaptmp_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HGU4561462.1"
                     /translation="YEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWP
                     GRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGRSGSGTTLDNHFAVIPADRTWRP
                     QPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAW
                     AGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSK
                     TYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVT
                     NNQIQTVGVNQIPGWWTASGGR"
     gene            866..1194
                     /locus_tag="ACHOSM_RS26015"
                     /old_locus_tag="pgaptmp_005203"
                     /pseudo
     CDS             866..1194
                     /locus_tag="ACHOSM_RS26015"
                     /old_locus_tag="pgaptmp_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          272 a          342 c          389 g          191 t
ORIGIN      
        1 agtatgaagt gtatgactac cccggacgtt tcaagggtgc ccacgggcag aactttgccc
       61 gctggcagat ggacggctgg cgaaacaacg cagaagtggc gcgcggaaca agccgttcgc
      121 cggagatatg gccgggacgg cgaattgtgc tgacggggca tccgcaggcg aacctgaacc
      181 gggaatggca ggtggtggcg agcgatcttc acggcgagca gccgcaggcg gtaccgggac
      241 gcagtggttc aggcaccacg ctggataacc attttgcggt aataccggca gacagaacat
      301 ggcgaccaca gccgttgctg aaaccgctgg tggacggccc gcagagcgcc gtcgtgacag
      361 gaccggcagg cgaggaaatc ttctgcgacg aacatggccg cgtgcgggtg aaatttaact
      421 gggaccgtta taacccgtca aaccaggaca gttcatgctg gatccgtgtg gcacaggcgt
      481 gggcaggcac cgggtttggt aacctggcga taccgcgtgt gggtcaggag gtcattgtgg
      541 acttcctcaa cggcgatccg gaccagccga tcattatggg gcgtacctac caccaggaaa
      601 accgcacccc cggcagcctg ccggggacga agacgcagat gacgatccgc tcgaaaacct
      661 ataagggcag cgggtttaat gaactgaagt ttgacgatgc gacagggaaa gaacaggtct
      721 acatccacgc gcagaagaac atgaacaccg aggtgctgaa taaccgcacc acggatgtga
      781 taaacaacca tgctgagacc attggcaaca atcagatgat tgcggttacc aacaatcaga
      841 tacagacggt gggtgttaac cagataccgg gatggtggac cgcttcgggc ggacgctgac
      901 gtaccggcgt gaggccgccg gtgacctggc cggggaaatc accggcgtga cggacggtgc
      961 cgggcgggag ttccgtctgg tgctgaccac gcaggcgcag cgtgcggaag aggcccgtaa
     1021 acagcacacc gcttccttat cttcccctga cacaccccgt cctctctcag cctcagcgtt
     1081 ccccgacacg ctgcccggta cggaatacgg ccccgacagg ggtatccgcc tttcagcggt
     1141 gtggctgacg cacgaccggg catacccgga gagcctgccc ggtgcgccgc tggc
//
LOCUS       DAZKHC020000344         1189 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZKHC020000344 DAZKHC020000000
VERSION     DAZKHC020000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.350"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            37..84
                     /locus_tag="ACHOSM_RS26020"
                     /old_locus_tag="pgaptmp_005204"
     CDS             37..84
                     /locus_tag="ACHOSM_RS26020"
                     /old_locus_tag="pgaptmp_005204"
                     /inference="COORDINATES: protein motif:HMM:NF018255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561463.1"
                     /translation="MFPDEPIEFSIEYEN"
     gene            74..964
                     /locus_tag="ACHOSM_RS26025"
                     /old_locus_tag="pgaptmp_005205"
     CDS             74..964
                     /locus_tag="ACHOSM_RS26025"
                     /old_locus_tag="pgaptmp_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HGU4561464.1"
                     /translation="MKINDFYLPLLSVIGTGRLYITPEGHACHAYFREVSGNGIRFTL
                     TCSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTALPEGDVSVDD
                     VQFTMLNKDVYPVIENNNGENRLNVIILEITVQSLQYLINENWQFVPHSNTIFFDGYI
                     APGWTDYPVTELTVGDSLRLYHVDDSKERECWLVINNPLATVKLCDNNLFIADVQAAD
                     LLCTISNEAVMDRIYCVIGTIHVDIHMLRNAKKDDIIDSTGYHLFGGCRLIRNNTTIA
                     YGSIVKINEDFYFTVSLVCD"
     gene            995..>1189
                     /locus_tag="ACHOSM_RS26030"
                     /old_locus_tag="pgaptmp_005206"
     CDS             995..>1189
                     /locus_tag="ACHOSM_RS26030"
                     /old_locus_tag="pgaptmp_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspH"
                     /protein_id="HGU4561465.1"
                     /translation="MSSSLSGIILNTSLTSHHMSWGKFSRTLHVLNEDIVDEIQLKAR
                     TQQRHHRVYSEMADYSVVSFR"
BASE COUNT          372 a          189 c          246 g          382 t
ORIGIN      
        1 cataaaaaga attatatttg taataagtta aaagaaatct ttcctgatga acctattgaa
       61 ttctcaattg aatatgaaaa ttaatgattt ttacttgcca ttgttgtcag tcataggaac
      121 cggtcgcctc tatattacgc ctgaagggca tgcttgccat gcatattttc gcgaagtcag
      181 cggtaatggt atccgcttca cgttaacatg ctcaggctat gaggggcgtt tttggatatc
      241 agaagaacag ttcattcagt ggtgccagga gttattcccg tatagcgaaa gccggctgat
      301 accggaagat atgataaaac tcatgatctt atgggtaatg cagactgctt taccagaagg
      361 tgatgtgtct gttgatgatg ttcaatttac gatgctaaat aaagatgttt atccggtaat
      421 agaaaataat aatggagaaa acagacttaa tgtaattata ctcgaaataa cggtacagag
      481 tttacaatat ctaattaatg aaaattggca atttgttcca cattctaata ccattttctt
      541 tgatggatat attgctcctg ggtggactga ttacccggtt acagagctga ctgttggtga
      601 tagtttaagg ctctatcatg ttgatgacag caaagaaaga gagtgttggc ttgttattaa
      661 taacccttta gcaaccgtaa aactttgtga taataattta tttattgcgg atgtccaggc
      721 tgcagatttg ttatgtacta tcagcaatga ggctgtaatg gatcgcattt attgtgttat
      781 tggaacaatt catgttgata tacatatgtt gcgcaatgcc aaaaaagatg acattatcga
      841 ttcaactgga tatcatcttt ttggtgggtg ccggttaata cgcaataata cgactatagc
      901 atacggaagc attgtaaaaa tcaacgaaga tttttatttt actgttagtt tagtatgtga
      961 ttaatcagga ataagttata tagggagatg tattatgtcg tcatcgttat caggaataat
     1021 actcaatact tcgctgacaa gtcaccatat gtcatggggc aagtttagca gaactcttca
     1081 tgtattgaat gaagatatcg tagatgaaat tcagcttaaa gcccgaacac aacaacgcca
     1141 ccatcgagta tattcagaaa tggcggatta ctccgttgtc tcctttcgg
//
LOCUS       DAZKHC020000345         1185 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZKHC020000345 DAZKHC020000000
VERSION     DAZKHC020000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.351"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..663
                     /locus_tag="ACHOSM_RS26035"
                     /old_locus_tag="pgaptmp_005207"
     CDS             <1..663
                     /locus_tag="ACHOSM_RS26035"
                     /old_locus_tag="pgaptmp_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4561466.1"
                     /translation="ENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPI
                     GLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQL
                     RLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDPQYPGMETKTA
                     SDYLPQTNWSTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGI
                     RK"
     gene            660..>1185
                     /locus_tag="ACHOSM_RS26040"
                     /old_locus_tag="pgaptmp_005208"
     CDS             660..>1185
                     /locus_tag="ACHOSM_RS26040"
                     /old_locus_tag="pgaptmp_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561467.1"
                     /translation="MKRVLFFLLMIFVSFGVIANCETQAKDQDCFTIFTKGTIFSAFP
                     VLNNKTMWRWYQNEDIGEYYWQTELGTCENNKFTPSGARLLIRVGSLRLNENHATKGT
                     LQELLNTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYEC"
BASE COUNT          383 a          240 c          262 g          300 t
ORIGIN      
        1 gagaacccgc atcatctgca gcagctcatt cgcctgccgg ggcagcagta tgatgaggag
       61 tccggcctgt attacaaccg ccaccgctat tatgacccgc tgcaggggcg gtatatcact
      121 caggatccga ttggactgaa ggggggatgg aatttttatc agtatccgtt gaatccggtt
      181 cagtatatag attcaatggg gctggcatct aaatatggac acttaaataa tggcggatat
      241 ggagcgaaac ccagcaaacc gcctacgccc gatccaagta agttgccaga catagcgaaa
      301 caattaagac tgccatatcc tattgaccag gccagtagtg cgcctaatgt tttcaaaaca
      361 ttcttcagag cattaagccc ttacgattac acactgtatt gcaggaagtg ggtaaaaccc
      421 aatctgactt gtacgccgca ggatgatccc cagtatccag ggatggagac aaagacagca
      481 agtgattacc tgccacagac aaattggtca acaactcaat taccaccagg atatacttgt
      541 gcagaaccct atttattccc agacattaat aaacccgatg ggccagcaac agcagggata
      601 gatgatttgg gtgaaatttt agctaaaatg aaacagagaa catcgagagg aataagaaaa
      661 tgaaaagagt tttgttcttt ttgctgatga tattcgttag ttttggtgtt atagctaatt
      721 gcgaaacaca agctaaagat caagattgct ttaccatttt cactaagggt actatttttt
      781 ccgcgtttcc tgtcttaaat aataaaacca tgtggagatg gtatcagaat gaggacatag
      841 gtgagtatta ttggcaaaca gaacttggta catgtgaaaa taataaattc acaccaagcg
      901 gggcgagact cttaattcgc gtcggatcac tacgccttaa tgaaaatcat gcaaccaaag
      961 gaaccttgca ggagttgcta aatacagcag aaaaaactgc gtttctcggc gatcggttta
     1021 gaagttatat aagagcgggt atctatcaaa aaaagagcag tgatccagtt cagttgttgg
     1081 cagtccttga taattcaatc atggtaaaat attttaaaga tgagaaacca acttatgctc
     1141 gaatgacagc acatctcccg aacaaaaatg agtcttacga atgtt
//
LOCUS       DAZKHC020000346         1180 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZKHC020000346 DAZKHC020000000
VERSION     DAZKHC020000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.352"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            100..372
                     /locus_tag="ACHOSM_RS26045"
                     /old_locus_tag="pgaptmp_005209"
     CDS             100..372
                     /locus_tag="ACHOSM_RS26045"
                     /old_locus_tag="pgaptmp_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561468.1"
                     /translation="MDNNTISLQELLDCISKLRDDVNALTVAFSYLALSIPREQMQPT
                     LASLLLESRNPKWSQEQQNSFKWLAALLEEKYAGKITISVESSENQ"
     gene            complement(338..682)
                     /locus_tag="ACHOSM_RS26050"
                     /old_locus_tag="pgaptmp_005210"
     CDS             complement(338..682)
                     /locus_tag="ACHOSM_RS26050"
                     /old_locus_tag="pgaptmp_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HGU4561469.1"
                     /translation="MTQNYELIVKGIRNFENKVAVTVALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLEVAAGLCEGDEQSPEKRPVILEAQNVWITYKGKLPG
                     RITDSLKTPPKW"
     gene            complement(687..902)
                     /locus_tag="ACHOSM_RS26055"
                     /old_locus_tag="pgaptmp_005211"
     CDS             complement(687..902)
                     /locus_tag="ACHOSM_RS26055"
                     /old_locus_tag="pgaptmp_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4561470.1"
                     /translation="MKSMDKLTTGIAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKVARGE"
BASE COUNT          337 a          278 c          222 g          343 t
ORIGIN      
        1 ttatcaggat ttatcggttt ctgaaccctg gatatgttta tctgtcccgg cctgttgaat
       61 caggcgggga aaaggtaaag acaatcaaga ggattattta tggacaataa caccatttct
      121 ctacaggagt tgctcgactg catttcaaag cttcgggatg atgtaaatgc cctcactgtt
      181 gcattttcat atttggcctt atcaattccc agagaacaaa tgcaaccaac actggcatcg
      241 ctcctgcttg aatcacgcaa ccccaaatgg tcccaggaac aacaaaattc tttcaagtgg
      301 ctggcggcat tactggaaga aaaatatgct ggtaaaatta ccatttcggt ggagtcttca
      361 gagaatcagt aattcttcct ggtagctttc ctttgtaggt tatccacaca ttctgcgcct
      421 ctaaaattac ggggcgcttt tccggcgact gctcatcccc ttcacataac ccggctgcaa
      481 cttccaggaa gacctgtctg atgctccttc tggctgctgc ctcataaaac tccagcgcgg
      541 caccttcaac acggtccagc gagatgtcca ggtcaaaaat ttcaccgtca aagcgttttt
      601 tgtcccttaa tgctacagtt accgcaactt tattctcaaa attgcggatc cctttcacaa
      661 tcagttcata gttttgtgtc attgaattac tctccccgtg caaccttacg cttgtcttct
      721 ttaatcttga aataaaggtt tgtcaggtac gtcagcaagc caaataccag actacccagc
      781 acaccgattg cagcccactg tgacggagtt actttatcga gtaactgcaa tgcccagaaa
      841 ccagcattac cagccgatgt gccataggcg atacctgttg ttaacttatc cattgatttc
      901 atatcctcac cccgatgtac acagatggtg caatatgttt gaaaagatcg gagtctatgg
      961 ggtagttttt atagcaaacg ttgttctcaa cggctctaaa aaacagacac attaaaaatg
     1021 tgggtaatta ttttaaaaga aaatcatata ttaaataata atacgagata tgttttcata
     1081 tttagtgtac tgtatacggc catttataca ggaaaagcct atgtcagaac gtaaagactc
     1141 aaaatcacgc cgtaattatc tcgttaaatg ttcctgccca
//
LOCUS       DAZKHC020000347         1153 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZKHC020000347 DAZKHC020000000
VERSION     DAZKHC020000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.353"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            676..1153
                     /locus_tag="ACHOSM_RS26060"
                     /old_locus_tag="pgaptmp_005212"
                     /pseudo
     CDS             676..1153
                     /locus_tag="ACHOSM_RS26060"
                     /old_locus_tag="pgaptmp_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545887.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          334 a          218 c          247 g          354 t
ORIGIN      
        1 aaactaaata taaataacga ctgcctcggc atgccagtta acagctatcc gtatggcgtg
       61 ttcggttttg cgaataaaaa atggttactg tagaagcgga tttccaccac atgtcaacca
      121 tgaatggtca gtggtgcgag acgactggga tgatttggtc gatgacttct tccggcgaat
      181 actgatagaa acattgaaat attgtaagag aaagcgttca aaattctgtg gcacgtgaaa
      241 aattctacag cgtcatcacc ttattcagtc gaccttcaaa ataaacgttt aacagagaca
      301 atcaccattt ggattgtccg ctgaggaggc gaacaatttc ctagacagct ttatcatgtc
      361 agcccacatt gttggcgctg acaatactaa ggacaaacga tggggaagta gctttttagc
      421 gttattttcg ggagtaaaaa acgtggtatc tttccgtatg tcagttgtat cttgctggta
      481 ttcgtagtcg tgataaggca tacaacgata tttatcttga cgttagtgaa gaataagttg
      541 ttttgtgatc tatgttaatc tgggattaca atccgggctt ggtacatcgc atcacgactt
      601 ttattctctt cagaattgaa tatgttactc cccgttttac aggctcagga tttgaattac
      661 aaattatact tttaagtgaa caataaccag gggtatttct tatttcctga aaccaggctt
      721 ggcccatttc gggatgagtt tgaaaaaact cttgacgcat acgatagtga tgaaataaac
      781 gaaacaaatt acgtcaacaa actcaggcgt cttgcctgac atcattgata ttcacgctca
      841 tctggcatat gcatttgcgc tctttatgct gcaattctgg cgaatgttca tttacagcgt
      901 catcaggatg ccgttatact cattgataaa atactaacat ataaccctga gaataatcag
      961 gggacacgct ggttactggg ggcggaattg ttacgtgctg gcaagcatgg cagggctctg
     1021 tacgtattga caggacatgt caatgaattt cccctctact ggtacgagct tgctctttta
     1081 cattttatga atgaggcata tattaatgct gcaacggcat ttcgatatgg atttttaaca
     1141 agtatatatt gtt
//
LOCUS       DAZKHC020000348         1151 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZKHC020000348 DAZKHC020000000
VERSION     DAZKHC020000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.354"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..548)
                     /locus_tag="ACHOSM_RS26065"
                     /old_locus_tag="pgaptmp_005213"
     CDS             complement(<1..548)
                     /locus_tag="ACHOSM_RS26065"
                     /old_locus_tag="pgaptmp_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4561471.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSEPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGA"
     gene            817..933
                     /locus_tag="ACHOSM_RS26070"
                     /old_locus_tag="pgaptmp_005214"
                     /pseudo
     CDS             817..933
                     /locus_tag="ACHOSM_RS26070"
                     /old_locus_tag="pgaptmp_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1038..1114)
                     /locus_tag="ACHOSM_RS26075"
                     /old_locus_tag="pgaptmp_005215"
     tRNA            complement(1038..1114)
                     /locus_tag="ACHOSM_RS26075"
                     /old_locus_tag="pgaptmp_005215"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1078..1080),aa:Arg,seq:tct)
BASE COUNT          274 a          319 c          302 g          256 t
ORIGIN      
        1 gcgccctggg tgaatgcaga accaccacga cagcatggta ccagcaggat ccccgcgtta
       61 ttcgggatat acggaagcag ttttttggca atatgtaagc cctggccgac acagccgtac
      121 tgccctttgc tcaggtctgc cttcggatga ttcagcgtac tcatatcctg cacatcatgc
      181 aggcagtggt cagccggaat gatgtcgtta tacgtacaac tctctccacc cggagttacc
      241 gtgctgcggc gcgccagctg tttaatgcgc ggatccggag catcgtaaga atccggtaac
      301 ggaagccctt caccgtaggc catgccgttg gactgtccgg caagcacaac cacgtagaac
      361 cagtccggct cagatgaagg gccgacctgt ggatctcctt caatagccac cgcctgcatc
      421 agtgtgtacg gcgtaatggc aaccggtccg ccgtatggct gccagccctc tttcagtttg
      481 tgtgtcagct tttccgcaag gtctgacggc gacgccgccc tgacaacatc atagtgttta
      541 aatgccatga atcctcccgg ccgggataat attgtgagta aaatgaggag cgggctgaag
      601 tccggaagtt acaggacaat ggcagaagag agacaacagc ccgcaatacg aaaaaggccg
      661 cgctattgcg cagagtgatt actgtcggat attattcgcc agctgaaata ttacttcacg
      721 ttttgttgtt tattccttgc cgcccgcgtc tccctgcgcg ggcttttttt gtccataaga
      781 aagcccctcc ggagaggggc tggagagtgg cgctatgtgc cattgcatgg tgccgggtgc
      841 ctcccggtga attcagtacc agcacctgaa tccgcgatta tcccatatac ctactcgctg
      901 attgcccctc cgcacagggg gattcaccat gccagtttct tttaacaaac tccccgcaaa
      961 ccagacaaca gtcaaccgcc tgaattgtga gacatttaaa aaaaaggccc gcaaaagcga
     1021 gccagggaaa ataagtgtgg cgcgttgtac tggattcgaa ccagtgaccg attgcttaga
     1081 aggcaattgc tctgtccggc tgagctaaca acgcaggata cagataatgg accgccttcg
     1141 gggacccgaa c
//
LOCUS       DAZKHC020000349         1150 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZKHC020000349 DAZKHC020000000
VERSION     DAZKHC020000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.355"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..539
                     /gene="rrrQ"
                     /locus_tag="ACHOSM_RS26080"
                     /old_locus_tag="pgaptmp_005216"
     CDS             6..539
                     /gene="rrrQ"
                     /locus_tag="ACHOSM_RS26080"
                     /old_locus_tag="pgaptmp_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4561472.1"
                     /translation="MNAKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLTKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGMASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDRGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            536..1027
                     /gene="rzpQ"
                     /locus_tag="ACHOSM_RS26085"
                     /old_locus_tag="pgaptmp_005217"
     CDS             536..1027
                     /gene="rzpQ"
                     /locus_tag="ACHOSM_RS26085"
                     /old_locus_tag="pgaptmp_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HGU4561473.1"
                     /translation="MNQIFTVILLVLVGFVVGNVWSDRGWQKKWAERDAAELSQEVNV
                     QFAARIIEQGRTISRDEAVKDAQQKAAEISARAADLSDSVNQLRAEATKYAIRLDAAQ
                     HTANLAAAVRGKTTKAAEGMLTNMLGDIAAEAQLYAEIADKRYIAGVTCQRIYESLRD
                     KTY"
BASE COUNT          336 a          232 c          307 g          275 t
ORIGIN      
        1 tatccatgaa cgcaaaaatc aaatacggcc tgtcagcggc cgttctggcg ctgattggag
       61 caggcgcatc tgctcctgag atacttgacc agtttctgga tgaaaaagag ggtaaccaca
      121 ctacggcata ccgcgatggt tccggcatat ggaccatctg tcgaggggcc acgatggtgg
      181 atggtaaacc tgttattcct ggcatgaaac tgacgaagga aaaatgcgat caggttaatg
      241 ccattgaacg ggataaggcg ctggcatggg tggagcgcaa tattaaagtg ccattgaccg
      301 aaccacagaa agcgggtatg gcgtcatttt gtccctataa cattggcccc ggtaagtgtt
      361 tcccgtcgac gttttataag cggctgaatg caggtgatcg taaaggtgcc tgcgaggcga
      421 ttcgctggtg gattaaggac agggggcgcg attgccgcat tcgatcaaat aactgttacg
      481 gtcaggttat tcgtcgtgac caggagagtg cattaacctg ctgggggata gaacagtgaa
      541 tcagatattc acggtgattt tgctcgtgtt ggtaggattt gtcgtaggta atgtctggag
      601 cgacagagga tggcaaaaaa aatgggcgga gcgtgatgct gccgaattat ctcaagaggt
      661 aaatgtccaa tttgctgctc gaataattga acaggggcga actatatccc gtgatgaggc
      721 tgttaaagat gcacaacaga aagccgctga aatttctgcc agggctgctg atctgtctga
      781 tagtgttaac cagctgcgtg ccgaagcaac aaaatatgcc atacgccttg acgcagcgca
      841 gcataccgca aatcttgccg ctgccgtcag aggcaaaaca accaaagccg ccgaaggaat
      901 gctcaccaac atgctcggag atattgcagc agaagctcag ctttatgctg aaattgctga
      961 caaacgctac atcgcaggag tgacttgtca acggatttat gaatctttaa gagataaaac
     1021 atattagatg tagattaaca ttaaatcgga ttatttttag cgctgaatgt gaaatttaaa
     1081 taaaaaggac tcttccatga gtcaaaatcc ttgaaatctt aagggtaaga taaaaggtca
     1141 ttagacagaa
//
LOCUS       DAZKHC020000350         1144 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZKHC020000350 DAZKHC020000000
VERSION     DAZKHC020000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.356"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..189
                     /locus_tag="ACHOSM_RS26090"
                     /old_locus_tag="pgaptmp_005218"
     CDS             <1..189
                     /locus_tag="ACHOSM_RS26090"
                     /old_locus_tag="pgaptmp_005218"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561474.1"
                     /translation="PPPKGSVVGMRRAWRGYKKGPQKRARENKCGALYWVRTSDRLLR
                     RLAFYPDELMAVGGPCWI"
     gene            complement(168..243)
                     /locus_tag="ACHOSM_RS26095"
                     /old_locus_tag="pgaptmp_005219"
     tRNA            complement(168..243)
                     /locus_tag="ACHOSM_RS26095"
                     /old_locus_tag="pgaptmp_005219"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(208..210),aa:Ile,seq:cat)
     gene            complement(557..1045)
                     /locus_tag="ACHOSM_RS26100"
                     /old_locus_tag="pgaptmp_005220"
     CDS             complement(557..1045)
                     /locus_tag="ACHOSM_RS26100"
                     /old_locus_tag="pgaptmp_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4561475.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKHAIASYYH
                     SVARPRKMLCRGGGRIQKPSLATCRREVDEILNASLFMIYPILDSTFKNRKRVEKIKH
                     VA"
     gene            complement(1036..>1144)
                     /locus_tag="ACHOSM_RS26105"
                     /old_locus_tag="pgaptmp_005221"
     CDS             complement(1036..>1144)
                     /locus_tag="ACHOSM_RS26105"
                     /old_locus_tag="pgaptmp_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561476.1"
                     /translation="AVKPLKFANQMYIDTGAVFCENLTLIQVQGEGAWA"
BASE COUNT          299 a          269 c          264 g          312 t
ORIGIN      
        1 ccccccccga aaggctctgt ggttggtatg cgccgggcgt ggcgcggata caaaaaaggt
       61 ccgcaaaagc gagcgaggga aaataagtgt ggtgcgttgt actgggttcg aaccagtgac
      121 cgattgctta gaaggctggc gttctatccc gatgagctaa tggcggttgg tggcccttgc
      181 tggatttgaa ccagcgacct ggcgattatg agtcgctcgc tctcaccact gagctaaagg
      241 gccgagcgta gaatgataac gttacgaaat caatgttgca agagttcaca aattcactga
      301 ttcaaaatta ttcttgtaaa aaatctttta acgtcaggca caaagagcct gtacatagat
      361 ttgtgtaatt gcctgatttt gatatgttca atccagcatc aaatgaaggt taatttatgg
      421 acgaaaaaca gttacaggct ctaatgacct ttatacgatt gacccaaata aaaaaagcca
      481 ccgttgcaac ttaagagtca ctaacggcag cttatgcgaa tagtgttgct catttgctca
      541 acgatgtcaa cacgttctat gctacatgtt taattttctc tacacgtttc cggtttttaa
      601 acgtactatc cagaatcggg taaatcataa acaacgaggc attgaggatt tcgtcaactt
      661 cccgtcgaca ggttgctagc gatggttttt gaatgcgccc gccgccccgg cataacatct
      721 tgcgaggtct tgcgacgcta tgatagtaag atgcaatggc gtgcttggaa gagccgtggg
      781 cgtagtagct gaggaggatg ccaaaggctt tcttgtcaat gtacatgacg gaatcgacga
      841 cctgagaaat caacattcca tcatcatcat tacacattgg ccttgtcata actcttcccg
      901 gctccacgct ctccatgaat ttagctatta cgctgctcat gcgcttttcc agacgacctg
      961 aataaaccca tgcgccccac agttcaagcc agccattcag ccactcatgc tgttctttgg
     1021 tgaggtttag ttctcttatg cccacgcgcc ttctccctgt acctgaatca atgtgagatt
     1081 ttcgcagaac actgcgccgg tatcgatata catctggttg gcaaatttga gtggtttcac
     1141 tgct
//
LOCUS       DAZKHC020000351         1113 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZKHC020000351 DAZKHC020000000
VERSION     DAZKHC020000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.357"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..66
                     /locus_tag="ACHOSM_RS26110"
                     /old_locus_tag="pgaptmp_005222"
     CDS             <1..66
                     /locus_tag="ACHOSM_RS26110"
                     /old_locus_tag="pgaptmp_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4561477.1"
                     /translation="KLGMPLRIVNNYVYFDRRVSA"
     gene            63..290
                     /locus_tag="ACHOSM_RS26115"
                     /old_locus_tag="pgaptmp_005223"
     CDS             63..290
                     /locus_tag="ACHOSM_RS26115"
                     /old_locus_tag="pgaptmp_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561478.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            283..594
                     /locus_tag="ACHOSM_RS26120"
                     /old_locus_tag="pgaptmp_005224"
     CDS             283..594
                     /locus_tag="ACHOSM_RS26120"
                     /old_locus_tag="pgaptmp_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561479.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            722..940
                     /locus_tag="ACHOSM_RS26125"
                     /old_locus_tag="pgaptmp_005225"
     CDS             722..940
                     /locus_tag="ACHOSM_RS26125"
                     /old_locus_tag="pgaptmp_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HGU4561480.1"
                     /translation="MKHYLEKNYPRKGRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            942..>1113
                     /locus_tag="ACHOSM_RS26130"
                     /old_locus_tag="pgaptmp_005226"
     CDS             942..>1113
                     /locus_tag="ACHOSM_RS26130"
                     /old_locus_tag="pgaptmp_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4561481.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEM"
BASE COUNT          329 a          201 c          296 g          287 t
ORIGIN      
        1 aaattaggca tgccgctgcg catagtgaat aactacgttt atttcgacag gagggtgtct
       61 gcgtgaaaat cctgtatcag gattacggcc cggtggggca ggtggttatc agcagtactg
      121 taatggagtt tcggaagcat aaccgtgtgg tggatgctgt gctgttaacc tgtccgggga
      181 tatcggcgag tcgtgcaggt gtgtttatta tgaagacgaa attatatggc agtaaggcgt
      241 ggataaagaa ggcgtatcgt gtagcgttgc aggaggttaa cagtgagtga aattaaagaa
      301 atgccggtag ttcgtgacgg atatggctac tggacacatc ctgaatatga aaaattctgt
      361 gatggtcggg aatatatttc aacggaagag tttaacgcct ggatggagga aaataatctt
      421 caatacgtcc tctgcttcag agatgaagga tgtgctgacc ttgatgcgtg tgatgctgat
      481 atttctgcat gggaaccgga acgaccagag ggcgatggct ggtttattgg ttccattcat
      541 gatacggaag atggcccggt ttgtgtctgg ttgcgaaata aggctgaagc ataaaggcga
      601 taaaccaact aacaactaaa tactgaagat ttaaatcaga aacgattttt attaaatcct
      661 taaccggagg gattcctgca ccctcagaac atcaggaggc cgcccgaaag ggcggtaaga
      721 aatgaaacat tatttagaaa aaaattaccc acgaaagggc agaacaacag agtttctgtt
      781 tttcattctg tttatagtgt tgatgatacc gatatccccg ctattactgg tctggataat
      841 tggaaggaca tttgaaccag ttattgagct atataccgat gtgacatggg aatcattcag
      901 cgcactgcac aataaaatta atccgtataa ggaaaactga tatgagcact attaccagag
      961 aacgcgcgga gattaaatca tacatcacag gcttcctgag cgactcggcg cacgataaca
     1021 agtcttcaga cagcctgctg gctaatgtgt ttcgtatcgc gctggcatca ctggaagcag
     1081 agccgatagc aatggtagtg cctgatgaaa tgg
//
LOCUS       DAZKHC020000352         1108 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZKHC020000352 DAZKHC020000000
VERSION     DAZKHC020000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.358"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..248
                     /locus_tag="ACHOSM_RS26135"
                     /old_locus_tag="pgaptmp_005227"
     CDS             <1..248
                     /locus_tag="ACHOSM_RS26135"
                     /old_locus_tag="pgaptmp_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561482.1"
                     /translation="KFSITDDLMSMAVNELIKLTKIENNVDLNKFTTICTDVLSPLVT
                     RHNKEKKTNDILPFAKIPFLIFIEKMAIDTMNPGHTH"
     gene            473..964
                     /locus_tag="ACHOSM_RS26140"
                     /old_locus_tag="pgaptmp_005228"
     CDS             473..964
                     /locus_tag="ACHOSM_RS26140"
                     /old_locus_tag="pgaptmp_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4561483.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
BASE COUNT          442 a          172 c          165 g          329 t
ORIGIN      
        1 ataaattttc gataacagat gatttaatga gtatggctgt aaatgaactc ataaaactga
       61 ccaaaataga gaataatgta gacctgaata aattcactac tatatgcaca gacgttctat
      121 cccccctcgt caccagacat aataaagaaa aaaaaacaaa cgacattcta cccttcgcaa
      181 aaatcccctt tttaattttc attgaaaaaa tggcaattga tactatgaat ccaggtcata
      241 cacattaagg catttatgta atgtcgtaat taagataact aataaggtga atattagtaa
      301 aacaactaat gatgttatta gtttgtagtg aacagtactt ttaccaataa tgaaaaaaat
      361 accataagca atgcaagctt aaaactaata atttaaaaaa gataaatgta aaaatattaa
      421 tgagtcaatc aataataatt tagcatcacg agacacatca cagaggaata ttatgagcac
      481 agaaacaatt gaaatattca ataatagtga tgaatgggca aatcaactaa aacacgcatt
      541 atcgaaagga gaaaatctgg cattactaca tggtttaact cctgatatcc ttgatagaat
      601 atatgcatat gcattcgact accatgaaaa gggtaatata acggacgcag aaatttatta
      661 taaatttttg tgcatttatg cgttcgaaaa tcatgagtat ctaaaagatt ttgcatcagt
      721 atgtcagcca aaaaagaaat atcaacaagc atatgacctt tacaaactaa gttacaatta
      781 ctccccgtat gatgactatt cagttattta tcgtatgggt caatgtcaga ttggagctaa
      841 aaatatcgat aacgcaatgc aatgtttcta tcacattatt aacaattgtg aggatgatag
      901 tgttaagagt aaagcgcagg catatattga actcttaaac gataattcag aagataatgg
      961 ctaagcatta caatcatttg gttaaatttg ctggttatag tgcagttaac cactataacc
     1021 agcacctctt atgttttctt aaatcagcat cttaaaacag ttaagagcat tctataaatc
     1081 attatttttc aatgcgttag agaacaat
//
LOCUS       DAZKHC020000353         1105 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZKHC020000353 DAZKHC020000000
VERSION     DAZKHC020000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.359"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..751)
                     /gene="tssI"
                     /locus_tag="ACHOSM_RS26145"
                     /old_locus_tag="pgaptmp_005229"
     CDS             complement(<1..751)
                     /gene="tssI"
                     /locus_tag="ACHOSM_RS26145"
                     /old_locus_tag="pgaptmp_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HGU4561484.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTFCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDY"
BASE COUNT          305 a          267 c          276 g          257 t
ORIGIN      
        1 ggtaatcctg gtactggcct tcctgatcaa aacgccctgc ccagccgggt cgtttaaagg
       61 tgtagtcctt ggtcaccacg gaagaagggc ggatttgtgc gctgtagcgg aactggctga
      121 tgcagaaggt acttacctcg gtacgggtgt tcgggttcca ggggatctca aaggactccg
      181 gcagataacg cacggtgtcg cacaggacca ggctctggtc ggtacttttt tgcgcgtgct
      241 cctcataaaa gaagatgcct tcctccgccg ccatccggca caggaaatcg taatcagttt
      301 caccgtactg gacacaaaac tcacgggaag gatgcggctc gctgaacagc ggactccact
      361 cggtcacacc gttttccttc aggatcgtag cgaggatgct ttcgatgtcc tcattctgga
      421 agatacggaa gttctggcgc agccctgtgc gccacagcgg cgggcatacc ttcatgctgt
      481 acagcatctg gtttttgtcg ttctccccca gttcaaacca ggtcaccaca cctttcaccc
      541 ggcgctgtac gtcatcgccc tgccataccg tcaggtaggc cattttgtcc agcacctgcg
      601 caaattcaag ggaaagaaac tgctgactga ccagagaaag gtcgagggaa aaaagtgaag
      661 agagtgactg ggtgagatga aaggaaacca ccgcaaaagc atccggtggc aggccgtcca
      721 cttcaagtgt gaaacgtaat ccggttgaca tagcaacctc cgtgaaaatg aataataatc
      781 cgcacatatt gcatgtacaa catgtcgggc ggatgagata agcgagtcga catctggcgt
      841 taccggggaa tatcagaaaa atgtcatggt tatgtgattt tacttcagag aaacgggtgt
      901 ctgtttagtc aacaaaataa tcacaaacgc aacggatggg ttctcgacgt tattcacgac
      961 aggctcgtac attacagcga gtaataaaaa acctgatatg caaagtcaaa ccaatattca
     1021 taatgtcttt ttattcacaa caataaataa ttcaataaca atcattgaaa tgaatgatgg
     1081 tttgtataag gaatatacgc taccg
//
LOCUS       DAZKHC020000354         1097 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZKHC020000354 DAZKHC020000000
VERSION     DAZKHC020000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.360"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            549..737
                     /gene="dicB"
                     /locus_tag="ACHOSM_RS26150"
                     /old_locus_tag="pgaptmp_005230"
     CDS             549..737
                     /gene="dicB"
                     /locus_tag="ACHOSM_RS26150"
                     /old_locus_tag="pgaptmp_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4561485.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            734..925
                     /locus_tag="ACHOSM_RS26155"
                     /old_locus_tag="pgaptmp_005231"
     CDS             734..925
                     /locus_tag="ACHOSM_RS26155"
                     /old_locus_tag="pgaptmp_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4561486.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            1018..>1097
                     /locus_tag="ACHOSM_RS26160"
                     /old_locus_tag="pgaptmp_005232"
     CDS             1018..>1097
                     /locus_tag="ACHOSM_RS26160"
                     /old_locus_tag="pgaptmp_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4561487.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATA"
BASE COUNT          307 a          231 c          262 g          297 t
ORIGIN      
        1 tatcaattaa tcattaattt attcacaggt gaggtagagt gcgtgcgccg gacacggata
       61 agaatccggc actgacagtt tactgaaaag gatatatccc tgaaaagtca gggcataaca
      121 cgaaagcgcc cggcgaagtt agtctctctg tataggtcgt cgttaaattt aattcgatcg
      181 tgcgcttccg gttgtggcaa tccgcgaaat ggcgcggcgg taagtatggc ggggttattc
      241 cttccccgtt gaggacaccg ggttgtcagg ttgaccatac gcttaagtga caaccccgct
      301 gcaacgccct ctgttatcaa ttttctggtg acgtttggcg gtatcagttt tactccgtga
      361 ctgctctgcc gcccttttta aagtgaattt tgtgatgcgg tgaatgcggc tgagcgcacg
      421 cggaacagtt aaaaccaaaa acagtgttat gggtggattc tctgtatccg gcgttaattg
      481 ttaactggtt aacgtcacct ggaggcacca ggcaccgcat cacaaaattc attgttgagg
      541 acgcgataat ggaaacgtta ttaccaaacg ttaatacgtc tgaaggttgt tttgaaattg
      601 gtgtcactat cagtaaccct gtatttactg aagatgccat taacaagaga aaacacgaac
      661 gggagctatt aaataaaata tgcattcttt caatgctggc ccgtttacgt ccgatacaaa
      721 aaggatgctg gcaatgaata cagcatttgc acttgttctg acagtttttc ttgtttccgg
      781 agagccagtt gatattgcag tcagtgttca caggacaatg caggagtgtg tgactgcagc
      841 aaccgaacag aaaattcccg gtaactgtta cccggtcgat aaagttattc accaggataa
      901 taacgaaatc ccggcaggtc tttaaaacag ttccgtaata aacatccgat ttcattctta
      961 tatgccagca atggcaggga tttgttcacc cttaaatctg taatgaggta aaacaaaatg
     1021 agtaaagtct ttatttgcgc cgccattccg gacgaacagg caataaagga agaaggtgca
     1081 gtcgctgtag ccactgc
//
LOCUS       DAZKHC020000355         1077 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZKHC020000355 DAZKHC020000000
VERSION     DAZKHC020000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.361"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..935)
                     /locus_tag="ACHOSM_RS26165"
                     /old_locus_tag="pgaptmp_005233"
     CDS             complement(<1..935)
                     /locus_tag="ACHOSM_RS26165"
                     /old_locus_tag="pgaptmp_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HGU4561488.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVQPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQIRDDALILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHALARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDLLPAPLPPYRVLTGMADRFGR
                     TLAYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARQQAVVAGAKRPDIPDS
                     LPESTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLA"
BASE COUNT          204 a          364 c          300 g          209 t
ORIGIN      
        1 gccagtggcg caccgggcag gctctccggg tatgccgggt cgtgcatcag ccacaccgcc
       61 gaaaggcgga tacccctgtc ggggccgtac tctgtgctct ccggcagtga atcaggaatg
      121 tccggtcgtt ttgctccggc gacgaccgcc tgctgccggg cctcttccgc ccgctgtgcc
      181 tgtgtggtca gtaccagacg gaactcccgc ccggcaccgt ccgtcacgcc ggtgatttcc
      241 ccggccaggt caccggcggc ctcacgccgg tacgccagcg tccgcccgaa gcggtccgcc
      301 atcccggtaa gcacccggta cggcggcagc ggcgctggca gtaagtcctc agcacccggc
      361 acccgctcag accaccccag tatccaccac ggcccctgtg cgctgttggt cgccaggtaa
      421 agatgcgggc ttaaccggat atccggtggc agcgacgccc acagccgtgc cagcgcgtgg
      481 ccgtccggct gcgctgcctt accgccacgc accagccaca ttgactcact gcggctgtac
      541 accgcctccc ccggcagcag cggctcaaag tgaatgctcc gcccgccgtt gtcgttgagt
      601 atcagtgcat catcgcgtat ctgtaagcgg atatcagaag gcgctttcca gccggggccg
      661 aaaacgccca ccggtgcagg cgtcttcgtc cggtagctgc tgtaggtgcg ggagagaatg
      721 aacggcagcg ggccgggcag cgcaaggtcc gtctcgccgg gctgtacttt tgcaccgagc
      781 agcgggttca ccgggttgcc cgaggtcatc ccgcccggac acaccgagca cgccacgcca
      841 gttggcgcgc caattcttac acctgccgaa ccctggacaa tgggaccgcc atactgagtc
      901 atatctccct gacgcgccgc tggttttccg ctcatataaa gtgccctttt agttattgtt
      961 catatccctg tgttagcaac aaaaaaaata aaatgacaca caagaccagc taatgtattt
     1021 atatattttt cacataaact tatttgttct caatcaacaa caactgttat tacaggt
//
LOCUS       DAZKHC020000356         1074 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZKHC020000356 DAZKHC020000000
VERSION     DAZKHC020000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.362"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            180..524
                     /locus_tag="ACHOSM_RS26170"
                     /old_locus_tag="pgaptmp_005234"
     CDS             180..524
                     /locus_tag="ACHOSM_RS26170"
                     /old_locus_tag="pgaptmp_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HGU4561489.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            646..918
                     /locus_tag="ACHOSM_RS26175"
                     /old_locus_tag="pgaptmp_005235"
     CDS             646..918
                     /locus_tag="ACHOSM_RS26175"
                     /old_locus_tag="pgaptmp_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561490.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            920..>1074
                     /locus_tag="ACHOSM_RS26180"
                     /old_locus_tag="pgaptmp_005236"
     CDS             920..>1074
                     /locus_tag="ACHOSM_RS26180"
                     /old_locus_tag="pgaptmp_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561491.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPT"
BASE COUNT          260 a          243 c          300 g          271 t
ORIGIN      
        1 cgaatccgaa ccggtcagac ggaggtcgct gtcttcgtag actacgaatc tgagaagtaa
       61 gagaccaggc gggggagtaa tctcccgcca cctctgatgt gtcaggcatc ctcaacgcac
      121 ccgcgcttta ccatactgaa aatgctgttt gaatgttcat ctctgaaaga ggactatgaa
      181 tgaaaaaggt attgattgca gcacttattt ccggtgtgtc ttttggcgct tttgcacagc
      241 agggtggttt ccaggggcca gaagcagagc gttcaacagt agcgcaggca aaagaactga
      301 aggatgatgc atgggttatc cttgaaggga gcatcgttaa aaaagtgggt gatgaacgtt
      361 atgagtttcg tgacaatagc gggacaattg tcacggatat tgatgacagc gtatgggccg
      421 ggcagaatgt ttctccgaaa gacaaagtaa gaattgaggg tgaaattgat aaagacctga
      481 gcagtgttga agttgatgta aaggcactga aattattaaa gtaaccgccc ctgcttgtta
      541 agcccgtctt actgacgggt tttctgtttg tacattccgg cgtattgcct tacaattcgc
      601 gcagtcagcc tgaacaactg acacctgctg tcaccggaga atccgatgac acaacacata
      661 aaatcccaca attctgaagc cgacccggaa attaagcagg ggaggcgttt tcgtgcgcct
      721 cagtatggct ggtttcacta tctgttctgt acgatcgatg aggcagatat gcttcaagag
      781 gcgtatctgc gtcgcggtgt ccgtgtggag cggagtctga acgctgatcg tctgacctgg
      841 accgtttctg tatatcttcc tgttcgtgca catctgccac ggacacatgc ctgctaccgt
      901 cagcgcgtct ggaggtaatg tgcgggtatt acttcgacct gttctggttc cggaactcgg
      961 gctggtggtc cttaagcccg gtcgtgaatc attgccagtt tttcatcgcg gcagggtgct
     1021 ggtggagccg gaaccgaaaa acatgcgggc gctgccatct ggagcggttc ctac
//
LOCUS       DAZKHC020000357         1066 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZKHC020000357 DAZKHC020000000
VERSION     DAZKHC020000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.363"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1066
                     /locus_tag="ACHOSM_RS26185"
                     /old_locus_tag="pgaptmp_005237"
     CDS             <1..>1066
                     /locus_tag="ACHOSM_RS26185"
                     /old_locus_tag="pgaptmp_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4561492.1"
                     /translation="NYYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARV
                     GYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGK
                     KHRDNTLTMKRFSNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFL
                     GDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEYYSAPVP"
BASE COUNT          254 a          265 c          306 g          241 t
ORIGIN      
        1 ccaattacta tctcccgaaa gaatccgcat accaggaagg gcgctgggaa acactgccct
       61 ttcagcgggc catcatgaat gcgatgggca gcgactacat ccgcgaggtg aatgtggtga
      121 agtctgcccg tgttggttat tccaaaatgc tgctgggtgt ttatgcctac ttcatagagc
      181 ataagcagcg caacacactt atctggttgc cgacggatgg tgatgccgag aactttatga
      241 aaacccacgt tgagccgacc atccgcgata ttccgtcgct gctggcgctg gctccgtggt
      301 atggcaaaaa gcaccgggat aacacgctca ccatgaagcg tttttccaat ggtcgtggct
      361 tctggtgcct gggcggtaaa gcggcaaaaa actaccgtga aaagtcggtg gatgtggcgg
      421 gttatgatga acttgctgcc tttgatgagg atattgaaca ggaaggctct ccgacgttcc
      481 tgggcgataa gcgtattgaa ggctcggtct ggccaaagtc catccgtggc tccacgccca
      541 aagtgagagg cacctgccag attgagcgtg cagccagtga atccccgcat tttatgcgtt
      601 ttcatgttgc ctgcccgcac tgcggggagg agcagtatct taaatttggc gacaaagaga
      661 cgccgtttgg cctcaaatgg acgccggatg atccctccag cgtgttttat ctctgcgagc
      721 ataatgcctg cgtcatccgc cagcaggagc tggactttac tgatgcccgt tatatctgcg
      781 aaaagaccgg gatctggacc cgtgatggca ttctctggtt ttcgtcatcc ggtgaagaga
      841 ttgaaccgcc tgacagtgtg acctttcaca tctggacggc gtacagcccg ttcaccacct
      901 gggtgcagat tgtcaaagac tggatgaaga cgaaagggga tacgggaaaa cgtaaaacct
      961 tcgtgaacac cacgctcggt gagacgtggg aagcgaaaat cggcgaacgt ccggatgctg
     1021 aagtgatggc agagcggaaa gagtattatt cagcgcccgt tcctga
//
LOCUS       DAZKHC020000358         1062 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZKHC020000358 DAZKHC020000000
VERSION     DAZKHC020000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.364"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..505)
                     /gene="yhaB"
                     /locus_tag="ACHOSM_RS26190"
                     /old_locus_tag="pgaptmp_005238"
     CDS             complement(<1..505)
                     /gene="yhaB"
                     /locus_tag="ACHOSM_RS26190"
                     /old_locus_tag="pgaptmp_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HGU4561493.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAHFNKTSIT
                     SDCWQILF"
     gene            complement(761..979)
                     /gene="tdcR"
                     /locus_tag="ACHOSM_RS26195"
                     /old_locus_tag="pgaptmp_005239"
     CDS             complement(761..979)
                     /gene="tdcR"
                     /locus_tag="ACHOSM_RS26195"
                     /old_locus_tag="pgaptmp_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HGU4561494.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          322 a          189 c          199 g          352 t
ORIGIN      
        1 aaaagagaat ttgccaacaa tctgaagtaa tgctggtttt attaaagtgc gcaggaggtg
       61 ttaagatggc ttcttttcgg gtgaattttg cacaatactt agaaatcgtc tccttaatac
      121 attccagctc ttttttattg acggctgagc aagtacattg ttcaccaaaa ggtgcaaaag
      181 tgcctacatc taatgtttgt atgatataat cgtcatgttc tcctgtagaa atatctatga
      241 tgaatggact catattttcc ggttggattc ggatcatgac agaaggaaca tcgccttgat
      301 agaaattcaa tgcagtaatt gcagataata ctctccgcag ttcattactt atactgtaaa
      361 ttttattaac gtcattggtg gagacaattt ctgcaaactt tctttttacc gtccaataat
      421 caatatttct attgtgattg ttaaccactg cgtgcattgg cgggattgaa gggtgaaaaa
      481 tatgtgcaat tgggaagcct ttcattgatg cctgccttgt cgtcatgaca aacaataata
      541 gatgcttatt ttcctcgcag ggttctttcg cactatcaaa aaacgctcat ttacccggcg
      601 ttacagtaaa cgaagcaaca ttctgtggcc tacgacgatc aaaagctctg tgaaaatttc
      661 ccttgttgtc tttgtctgac cttatcgcgg cgatcagcaa ttattcgttt tgtgatcctc
      721 aacacaaaat gctattcgtc gcctatgagg ggagaagaat ttatggaaaa cgattgcatt
      781 cacaaaatga cgtcctgtca gaagagaaaa agttgacgtt aatcaattgt tcgagatgat
      841 caatacctgt attcgaaatt ttaaaatatt gataaaataa atgtttgttc tccaacaaat
      901 agcgtgaaaa aatgtgacgt gccgcagtat ttatcgctga aaatggttta tttataataa
      961 aatttgagaa cttgcttatt aaatttaact caaattttgc ctggtaatta tccggtaatt
     1021 gcttgaaata tggtctcagc cccttttttg tattaaccac at
//
LOCUS       DAZKHC020000359         1056 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZKHC020000359 DAZKHC020000000
VERSION     DAZKHC020000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.365"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            82..474
                     /locus_tag="ACHOSM_RS26200"
                     /old_locus_tag="pgaptmp_005240"
     CDS             82..474
                     /locus_tag="ACHOSM_RS26200"
                     /old_locus_tag="pgaptmp_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4561495.1"
                     /translation="MRDIQQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGKTFMALARKHGCSDGHIGKRLQKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            complement(795..>1056)
                     /locus_tag="ACHOSM_RS26205"
                     /old_locus_tag="pgaptmp_005241"
     CDS             complement(795..>1056)
                     /locus_tag="ACHOSM_RS26205"
                     /old_locus_tag="pgaptmp_005241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561496.1"
                     /translation="YDHPYRNDDSRFAKGITKIIIPSAGGVRLRGLFVMLPTDSTRRR
                     INPRGVLTLAEEKHESVVFTGAVSGLFLANHSLHTWLFPVNL"
BASE COUNT          309 a          211 c          295 g          241 t
ORIGIN      
        1 tatatacccc gaattacggg gatgtacgga gttactgtac ggagttaccg ttgccaataa
       61 ttattcgcaa ggtgattgtt gatgcgtgat attcagcagg ttctggaacg atggggggcg
      121 tgggcggcaa ataactatga ggatgttaca tggtcgccca ttgctgccgg atttaaggga
      181 ctgatccccg aaaaagtaaa atcacgtcca cagtgttgtg acgatgacgc gatgattata
      241 tgcgggtgta tggctcgcct taacaggaac aacagcgatc tgcatgactt gctggttgat
      301 tattacgtgt tggggaaaac gtttatggcg ctggcacgta agcatggatg ctctgatgga
      361 catatcggaa agcggctaca gaaagcggaa ggtgttgttg aaggcatgct gatgatgctg
      421 gatgtgaagc tggagatgga tcggtatgtt gagcgcgatt cagtgacggc ataacgcgca
      481 taaaaaaaac cgcagtcatg aggaaactgc ggcggagaag tcagacttgc caatcaatca
      541 tgaaaaacgt aatcgcctga taattatgcc acgaagcacg gaaaaacagt actggtgcta
      601 agacatgaac catttgcatt gcaataagtt aagccgaaat gcgtcgggtc ctgaatggtg
      661 ataatcacca cgcagacaat caaagctacc ataacgaaca tcgttacagt tggagctttt
      721 accccgttgg ggaaaaggtg cacatctgga tcatcattat ggtgatcgag ggtatgtaaa
      781 gcccaccagc ctgattaaag gttaaccgga aaaagccagg tatggaggga gtggttagcc
      841 aagaacaagc cggacaccgc gccagtaaaa acaacagatt cgtgcttttc ttcagccagg
      901 gtaagaactc cacggggatt aatccgtcgt ctggtggaat cagtcggcag catgacgaac
      961 aggccgcgca gccggacacc gccagcagaa ggtattatga ttttcgtaat acctttagca
     1021 aagcgtgaat catcattacg gtagggatgg tcataa
//
LOCUS       DAZKHC020000360         1049 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZKHC020000360 DAZKHC020000000
VERSION     DAZKHC020000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.366"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(15..>1049)
                     /gene="wzy"
                     /locus_tag="ACHOSM_RS26210"
                     /old_locus_tag="pgaptmp_005242"
     CDS             complement(15..>1049)
                     /gene="wzy"
                     /locus_tag="ACHOSM_RS26210"
                     /old_locus_tag="pgaptmp_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032204448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O177 family O-antigen polymerase"
                     /protein_id="HGU4561497.1"
                     /translation="VVPFLNDVKLEYAITELLSFYGILLCPLIAQNRYISVNWKKIRK
                     FILFICIISAFIHILIWLLGLMGSGYVDLIKQLCIRVLTANNDDLIDNIIIADTPDGL
                     FRVLLPNSSLLIIGFYLSFQQYLNRKNYIHLLIVFVMLLALYTTWTRALYLSPIIIIG
                     GVLFYKIFPFTLQLGKIGAYNLLSFIILFFIVISTVLVQPTLLSLLGIASESSDGIRY
                     TQVIWIFNTFMNSPVLGTGLGGSAEDVRSLIAPWTYEMSLLALIMKLGLVGVFLFISI
                     SAIKIPEFLEGFFDGRRIPVKKVVWIYFSLSVLMMFSTNPFMLSFPGVTITLFILCEL
                     NSFKIEKGNI"
BASE COUNT          412 a          197 c          137 g          303 t
ORIGIN      
        1 gacagctcgt tgtatcatat attacctttc tcgattttaa agctattcaa ctcacataaa
       61 ataaacaaag taattgttac ccctgggaat gaaagcataa atggattggt tgaaaacatc
      121 attagcacag ataaagaaaa ataaatccat acgacttttt taactggtat ccttctccca
      181 tcaaaaaaac cctccaaaaa ttcaggtatt tttatagcac taatagaaat gaaaaggaaa
      241 acaccaacta gacctaattt cataatcagc gccaataaac tcatctcgta agtccatgga
      301 gcaattaatg aacgtacgtc ttctgcactc ccccccaagc cagtacccag aacaggacta
      361 ttcataaacg tattaaatat ccaaataacc tgtgtatatc ttattccatc actactctca
      421 cttgctatgc ccagcaatga taataaagtt ggttggacta gaacagtaga tatcacaata
      481 aaaaagagaa ttataaatga tagcaagtta tatgcgccaa ttttacccaa ttgcaaagta
      541 aatggaaata tcttataaaa aagtacacct ccaataatta taatcggaga taaatataaa
      601 gccctcgtcc atgtagtata taaagccaat agcattacaa atacaattaa taagtgtatg
      661 taattttttc tattgaggta ctgttggaat gatagataaa atccgataat caataaggaa
      721 ctattaggca gcaacactct aaataatcca tccggtgtat cggcaataat aatattatca
      781 attaagtcat cattatttgc agtcaatacc cttatacata attgcttgat aagatcaaca
      841 taaccagacc ccatcaatcc taagagccat attaatatat gaataaatgc acttatgatg
      901 caaatgaata gtatgaactt ccttattttt ttccagttaa ccgaaatata tctattttgc
      961 gcaattagag ggcatagcaa aataccataa aaagacaaaa gttctgttat tgcatattct
     1021 aacttaacat catttaaaaa aggaacaac
//
LOCUS       DAZKHC020000361         1044 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZKHC020000361 DAZKHC020000000
VERSION     DAZKHC020000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.367"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            5..565
                     /locus_tag="ACHOSM_RS26215"
                     /old_locus_tag="pgaptmp_005243"
                     /pseudo
     CDS             5..565
                     /locus_tag="ACHOSM_RS26215"
                     /old_locus_tag="pgaptmp_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430189.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
     gene            769..>1044
                     /locus_tag="ACHOSM_RS26220"
                     /old_locus_tag="pgaptmp_005244"
     CDS             769..>1044
                     /locus_tag="ACHOSM_RS26220"
                     /old_locus_tag="pgaptmp_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561498.1"
                     /translation="MNEIRSNPVKFLEEHLILNTQRYAHHDETSLITVNITREDGLLR
                     LKETEYDMAGSDYILFTAMRDTDSPERFDAPAVVSLSNQCISLRRNDI"
BASE COUNT          322 a          184 c          230 g          308 t
ORIGIN      
        1 agatattaat tcatcttcgt tccatcttgg aatggaggtt cttcgtgctc aagttgcagc
       61 cactgggcgt ggagaattta caatgggtgg tgaaactgtc agaattgaat atagtccaac
      121 agatgggcgc tttctggcca gcgatggcac tgggggatta tttactgaat tattgctttt
      181 agggttcaat aatgggcctc aagctcttgg cgagagaatg ttaagtattc tttcaggatc
      241 agatgcaggt gaaacacaat cgcaagtgac tcctcaggat aaaatatatc aatgtaagtt
      301 ttctgttaat acagagagcc tccagtgtcc ctctgatgca actcgatgcc caattatact
      361 ggaaacacca gaagaagggg tatttgtcaa aaattcagat agttcagcag tatgcacttt
      421 atttgatgtt gatgcacttt ctcgcgtggt taatgacggt tcagttcacc ctctgacacg
      481 agctccaata accccatcaa tgattgttaa accagaagag tgtaaatatg accctgcaag
      541 gggaagcttt attataaaag atagtcaaga tgtttcaaat gaaacaatat taacttccga
      601 taatttatat aaaaacacca caggcattcg gggcctgtgg ttggtaaaac aatataatac
      661 gcgagttatt tttcatgagc tggagagaaa acaatcaagg taagtagtat tatttcacaa
      721 aataccgggc actttctggt gccgcatcgt tcagagcttc ggtgtataat gaatgaaata
      781 cgtagtaacc ctgtaaaatt tctggaagag catttaattc ttaatacaca gcgctatgcc
      841 catcatgatg agacatcttt aattacagtt aatattacaa gagaggatgg cttgttaaga
      901 ctaaaagaaa cggaatatga tatggctggt agtgattata ttctttttac agctatgcgt
      961 gatactgata gtccggagcg ctttgatgca cctgcagttg taagcttgag taatcagtgt
     1021 ataagcctcc ggcgaaatga tatt
//
LOCUS       DAZKHC020000362         1042 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZKHC020000362 DAZKHC020000000
VERSION     DAZKHC020000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.368"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..654)
                     /locus_tag="ACHOSM_RS26225"
                     /old_locus_tag="pgaptmp_005245"
                     /pseudo
     CDS             complement(1..654)
                     /locus_tag="ACHOSM_RS26225"
                     /old_locus_tag="pgaptmp_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            666..818
                     /locus_tag="ACHOSM_RS26230"
                     /old_locus_tag="pgaptmp_005246"
     CDS             666..818
                     /locus_tag="ACHOSM_RS26230"
                     /old_locus_tag="pgaptmp_005246"
                     /inference="COORDINATES: protein motif:HMM:NF018671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU4561499.1"
                     /translation="MEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMT
                     EGELIR"
BASE COUNT          259 a          236 c          258 g          289 t
ORIGIN      
        1 cagtttttgc cagatagttg aaacgtattt tgcctggtaa cgggcatcat ccagcgcatt
       61 gtggcgctca ccttcgaatg ggatagcagt tctggcatcg aagtctacgg ctttccccag
      121 ctcaacgatt gtgcgtacat cgcgatcgtt gcagtagcgc cacgggcagg ggataccctg
      181 tcgttcgtat gaacggcgca aaatcgtgtt gtcgaagttg gccccattac cccagacctg
      241 aacaaaaaac tcaccggagt tttcgtcgat aaattcccgc aattgcagta gtgcatcatc
      301 taacgggatt tcatcggtca taatggcaga ctgcgcttca cgtgattgct tcagccacca
      361 tttaatgacg tcacgatcaa tgactccgcc agcagtatcc agatcgatgg tcttgctaaa
      421 ttctggtccc atatctccgg tttgcggatc gaaaaatatt gcgcctattg agataatcgg
      481 ggcatcggga ttttttccca tggtttcaag gtcgatcatc agatgaatcc ccgctctgct
      541 ggtggatgtg agattatgat gactgttcgc cttaattaag ggatctgacg cctcgccagt
      601 ttcactatcg ctggcatgat gctgattgcc gccagtgttc tccttgtgct gatgtggatg
      661 ccgtgatgga ggagtaccgt cgctggcgca acgagtcctg gcgatacgac gggagcgata
      721 aatacccgtg gtctcagcct gtgctgtatc acatttgcct cgagatgcgt tcaaagggga
      781 ttgagcggca gatgaccgaa ggggagttaa tccggtaaga gccggaaaaa gcatgccgcc
      841 tttaattctc agtggaagat ggacttgatt atactcattg cattgtcaga aatagttatc
      901 cattaaaggc tggctattcc aaacaggatg ttgattacaa aaatgtaatc aacatgtaag
      961 gtttatactc ttcaatatgc gtataatttt ccttattttg ttaactttaa ataacaagct
     1021 atgcacgagg taaagtcgga ta
//
LOCUS       DAZKHC020000363         1019 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZKHC020000363 DAZKHC020000000
VERSION     DAZKHC020000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.369"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1019
                     /locus_tag="ACHOSM_RS26235"
                     /old_locus_tag="pgaptmp_005247"
     CDS             <1..>1019
                     /locus_tag="ACHOSM_RS26235"
                     /old_locus_tag="pgaptmp_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4561500.1"
                     /translation="LVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETA
                     LGVEVTKAKPVTRTITSANIDRLRVTFGVQSLVQTTSKGDRNPSSVRLLIQLQRNGNW
                     VTEKDVTINGKTTSQFLASVILGNLPPRPFNIRMVRETADSTSDQLQNKTLWSSYTEI
                     IDVKQCYPNTAIVGLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGS
                     LKPAYSNNPAWCLWDMLTHPRYGMGKRLVAADVDKWALYAIGQYCDQTVPDGFGGTEP
                     RMTFNAYLSQQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNCDVVVD
                     DNGVG"
BASE COUNT          223 a          279 c          324 g          193 t
ORIGIN      
        1 ctggtgaaca aaaccccgct gacggacacg gacggtaatc ctgtgataca tggtgtgaca
       61 gcggtctggc gcgccgggga gcaggagcag acaccacctg aaggctttga gtcctccggg
      121 gcggaaaccg cactgggcgt ggaagtgacg aaggcaaagc cggtgacgcg caccattacg
      181 tccgcgaaca ttgaccgcct gcgggtcacc ttcggggtgc agtcactggt gcagaccacc
      241 tcaaagggcg accgtaatcc ctcttctgtc cgactgctga ttcagttgca gcgtaacggt
      301 aactgggtga cggaaaagga tgtcaccatt aacggcaaga ccacctcaca gttcctggcc
      361 tcggtgattc tgggtaatct gcctccccgt ccttttaaca tccggatggt ccgggagaca
      421 gcggacagca cctcggacca gctgcagaat aagacgctct ggtcgtcata caccgaaatc
      481 atcgatgtga aacagtgcta cccgaacacg gcgattgtgg ggctgcaggt ggatgcggag
      541 cagtttggcg gtcagcagat gacggtgaac taccatatcc gcggtcgcat catccaggta
      601 ccgtcaaact atgacccgga aaaacgcacg tacagcggca tctgggacgg cagcctgaaa
      661 ccggcataca gcaacaaccc ggcctggtgc ctgtgggaca tgctgactca cccgcgctac
      721 ggcatgggaa aacgtctggt ggcggcggat gttgacaagt gggcgctgta tgccatcggg
      781 cagtactgcg accagacggt cccggatggt ttcgggggca cagagccgcg gatgaccttt
      841 aatgcgtacc tgtcacaaca gcgtaaggcg tgggacgttc tcagtgattt ctgctcggcg
      901 atgcgctgta tgccggtatg gaacggccag acgctgacgt tcgttcagga ccgcccgtcg
      961 gatgtggtgt ggccgtacac caactgcgat gtggtggtgg atgataacgg cgtggggtt
//
LOCUS       DAZKHC020000364         1017 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZKHC020000364 DAZKHC020000000
VERSION     DAZKHC020000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.370"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(129..>1017)
                     /locus_tag="ACHOSM_RS26240"
                     /old_locus_tag="pgaptmp_005248"
     CDS             complement(129..>1017)
                     /locus_tag="ACHOSM_RS26240"
                     /old_locus_tag="pgaptmp_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine protease"
                     /protein_id="HGU4561501.1"
                     /translation="NIITIPKNINDFFITDIFLDADSLIQYVQLEHPVSDIKAFIRKS
                     NKIVLSLRGNHSGSVKRIEFQGNNGGNINDFLGRIVMYTLDGIELELSANKESPVVAA
                     KIDIPKFEKYHGDIGILDPFNILENNNLYFVKENISEFDFGNYRVVSSQDTFIYKVTN
                     PGAQFYISPGKPSHIFGEGGCHYYFSGVKQPSHLIFLNNDNRNPETINVSSFTETSEE
                     VKIFANAKENKCTLKFIWSYGSFELILRPRNSLRIDLNTSETKISLSHGTLLLRDIFE
                     LSQRSSGDVLIYNTLWQYH"
BASE COUNT          318 a          185 c          156 g          358 t
ORIGIN      
        1 gtgtattttt agtcatcctg tttacctctt tctcagggag tttagtctcc aggatttccg
       61 gggcggttca gtcagcctcg atactccgct tcaataacgt tcagtgctca atgaataaga
      121 acgtaggctt aatgatattg ccagagagta ttatagatta gaacatcccc actagagcgc
      181 tgagataact cgaatatatc tcttaataac agagtaccat gacttaaaga tatttttgtt
      241 tccgatgtat ttaaatctat gcgtaagcta tttcttggtc ttaatatcaa ctcgaaagaa
      301 ccataggacc atataaattt taaagtgcat ttattttctt tcgcattagc aaatattttt
      361 acttcctcag atgtctctgt gaaacttgat acattaatag tctctggatt tctgttatca
      421 ttatttaaga aaataagatg agacggttgc tttactccac taaaatagta gtgacatcct
      481 ccctctccaa aaatatgtga tggttttcct ggtgatatat aaaactgcgc ccccggattt
      541 gttactttgt atataaacgt gtcttgggaa gaaacaactc tataattacc aaaatcaaat
      601 tcactgatat tttctttgac gaaataaaga ttattattct ctaaaatatt aaaaggatcc
      661 aatatgccaa tatcaccatg atatttttcg aattttggta tatcaatctt tgctgcaact
      721 acggggcttt ctttattagc ggatagttct aactctatac catcaagagt atacattact
      781 atcctcccta aaaaatcatt tatgttgcca ccattattcc cttgaaattc aatgcgtttc
      841 acagaaccac tatgattccc tcttagtgat aaaactattt tattagactt cctgatgaaa
      901 gcttttatat cgcttacagg gtgttcaagt tgaacatatt ggataagact atcagcatcc
      961 aagaaaatgt cagtaatgaa aaaatcatta atgttcttag gaatggttat tatattt
//
LOCUS       DAZKHC020000365         1012 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZKHC020000365 DAZKHC020000000
VERSION     DAZKHC020000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.371"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            307..1012
                     /locus_tag="ACHOSM_RS26245"
                     /old_locus_tag="pgaptmp_005249"
                     /pseudo
     CDS             307..1012
                     /locus_tag="ACHOSM_RS26245"
                     /old_locus_tag="pgaptmp_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          304 a          257 c          236 g          215 t
ORIGIN      
        1 tatcttttta agcaagaagc atctaaaaac aaaaagctac cgcaaggaga atctctccca
       61 accaaaacaa cattagcgat aataatatat tgcctaaaca ttcttacaca tatcacagct
      121 aagttatatt ataacactca gatgattgat ttgtaagata acgtatgatt ttcatatgaa
      181 tagccacgtg acaaatgcct tattaaagag attgtaaaca gaatacagca aactatttca
      241 gtgatcttac ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg
      301 actcacatga caaaaacagt atcaaccagt aaaaaacccc gtaaacagca ttcgcctgaa
      361 tttcgcagtg aagccctgaa gcttgctgaa cgcatcggtg ttactgccgc agcccgtgaa
      421 ctcagcctgt atgaatcaca gctctacaac tggcgcagta aacagcaaaa tcagcagacg
      481 tcttctgaac gtgaactgga gatttctacc gagattgcac gtctcaaacg ccagctggca
      541 gaacgggatg aagagctggc tatcctccaa aaggccgcga catacttcgc gaagcgcctg
      601 aaatgaagta tgtctttatt gaaaaacatc aggctgagtt cagcatcaaa gcaatgtgcc
      661 gcgtgctccg ggtggcccac agcggctggt atacgtggtg tcagcggcgg acaaggataa
      721 gcacgcgtca gcagttccgc caacactgcg acagcgttgt cctcgcggct tttacccggt
      781 caaaacagcg ttacggtgcc ccacgcctga cggatgaact gcgtgctcag ggttacccct
      841 ttaacgtaaa aaccgtggcg gcaagcctgc gccgtcaggg actgagggca aaggcctccc
      901 ggaagttcag cccggtcagc taccgcgcac acggcctgcc tgtgtcagaa aatctgttgg
      961 agcaggattt ttacgccagt ggcccgaacc agaagtgggc aggagacatc ac
//
LOCUS       DAZKHC020000366         1006 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZKHC020000366 DAZKHC020000000
VERSION     DAZKHC020000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.372"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..449
                     /gene="yhaJ"
                     /locus_tag="ACHOSM_RS26250"
                     /old_locus_tag="pgaptmp_005250"
     CDS             <1..449
                     /gene="yhaJ"
                     /locus_tag="ACHOSM_RS26250"
                     /old_locus_tag="pgaptmp_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGU4561502.1"
                     /translation="APDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTR
                     VKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKD
                     IAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFSGK"
     gene            complement(500..856)
                     /locus_tag="ACHOSM_RS26255"
                     /old_locus_tag="pgaptmp_005251"
     CDS             complement(500..856)
                     /locus_tag="ACHOSM_RS26255"
                     /old_locus_tag="pgaptmp_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4561503.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
BASE COUNT          303 a          207 c          247 g          249 t
ORIGIN      
        1 tcgcgccgga tatgcatttt cgttcttcgt cggagatcaa ctcgcgcaag ctctatacgt
       61 taatgaacgt ctacgttgcc gcgcctgatc acccgattca tcaggagccg gaaccgttat
      121 ctgaagtgac gcgcgtgaaa tatcgtggaa ttgcggtagc ggataccgct cgtgagcgcc
      181 cggtgttgac cgtacagctg ctggacaaac agccgcgctt aacggtgagc acgattgaag
      241 ataaacgtca ggcattactg gcggggcttg gcgtggcgac gatgccgtat ccgatggtcg
      301 aaaaagatat tgcggaaggg cggttgcgtg tcgtcagccc ggaatcgacc agcgagatcg
      361 atattattat ggcctggcgt cgtgacagta tgggggaagc gaaatcctgg tgtctgcggg
      421 aaattcccaa actttttagc ggaaaataat atttattatt gttaataaaa aggggcgaaa
      481 tatcgcccca attctaagat taatttgaac caaacttcgg atcgtttccg taacgattgc
      541 tgccagaagt accttctgtg cagaaaaaaa ccagaagcac gagccagcca ataaacggaa
      601 caaaaaagag caaggcccag gcacctgaac ggtcggtatc gtgtaagcga cggatagcaa
      661 gcgcaaggac gggaaggaag gttgccagca aataaagcat agacagatat ggaagctcca
      721 gacctaaaat caattgaatg acattgataa tagcgccgac gatggcgtta atcagagtaa
      781 acatccagta ctctttacga cgtgcgcgac cagagaaacc aacataattt tttaatacgg
      841 ataaatacca ctgcataaaa acatcccttt aattaaaagt gacgaaagtc tattccgggt
      901 attttgtaaa tgctattcgt atctttcttt ttcggatgtt ataaaggttt tgatatttaa
      961 gataaataca tggttatttt ttataggtaa tttatataac gacatt
//
LOCUS       DAZKHC020000367         1005 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZKHC020000367 DAZKHC020000000
VERSION     DAZKHC020000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.373"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..785)
                     /locus_tag="ACHOSM_RS26260"
                     /old_locus_tag="pgaptmp_005252"
     CDS             complement(<1..785)
                     /locus_tag="ACHOSM_RS26260"
                     /old_locus_tag="pgaptmp_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4561504.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPSSEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQDLISRTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATHAQQPALFTAMLTQFRADLS"
     gene            762..899
                     /locus_tag="ACHOSM_RS26265"
                     /old_locus_tag="pgaptmp_005253"
                     /pseudo
     CDS             762..899
                     /locus_tag="ACHOSM_RS26265"
                     /old_locus_tag="pgaptmp_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          233 a          286 c          270 g          216 t
ORIGIN      
        1 gagaggtcag cacgaaactg tgtcagcatg gctgtaaaca gcgcaggttg ctgtgcgtgg
       61 gtggcggcgc tcatgtcaaa ctcaccctgc atccagcaga cggccagcag aacgtttttg
      121 ggatttttct gcaatgccgc ttttgtgcgg gaaatcagat cctgatataa cggcttgccc
      181 accccccagc gtgccgaatc ctgactggcc cccgtggact cgctgaatgt cccctccgcg
      241 ccctgggtaa atgccgaacc accacgacag catggtacca gcaggatccc cgcgttattc
      301 gggatatacg ggagcagttt tttggcaata tgtaaaccct ggccgacaca gccgtactgc
      361 cctttgctca ggtcagccct cggatgattc agcgtactca tatcctgcac atcatgcaga
      421 cagtggtcag ccggaataat atcgttatat ctgcaggcag ccccgcccgg cgtcactgta
      481 ctgcggcgcg ccagctgttt aatgcgcgga tccggagcat cgtatgaatc cggcagcgga
      541 agcccttcac cgtaagccat ggcattggac tgcccggcca gtacgatgac gtagtaccaa
      601 tccggctcag atgaagggcc gacctgtggc tctccttcaa tagccaccgc ctgcatcagt
      661 gtgtacggcg taatggcaac cggtccgccg tatggctgcc agccctcttt cagtttgtgt
      721 gtcagctttt ccgcaaggtc tgacggcgac gccgccctga caacatcata atgtttaatc
      781 gacatcgaat ttctcccgtg tacaggaaca gagttaaaaa gccggaatca aatcgcagga
      841 tgaacatctg ccagtggctg gtcgtaaaaa aaaggccgcg ccatgcgcag ccggaaataa
      901 agggataacg atgatagttt gagaaaaaca gaaataacac ttttgtggca aagcatggtg
      961 ccgggtgcct cccggtgaat tcagccggtg tcactgaacc cgcgt
//
LOCUS       DAZKHC020000368          989 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZKHC020000368 DAZKHC020000000
VERSION     DAZKHC020000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.374"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..387
                     /locus_tag="ACHOSM_RS26270"
                     /old_locus_tag="pgaptmp_005254"
     CDS             <1..387
                     /locus_tag="ACHOSM_RS26270"
                     /old_locus_tag="pgaptmp_005254"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4561505.1"
                     /translation="SDMQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALEN
                     GINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVT
                     FRHGTLRFNGNVSEKLLTLLIQELKR"
     gene            384..731
                     /gene="tnpB"
                     /locus_tag="ACHOSM_RS26275"
                     /old_locus_tag="pgaptmp_005255"
     CDS             384..731
                     /gene="tnpB"
                     /locus_tag="ACHOSM_RS26275"
                     /old_locus_tag="pgaptmp_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4561506.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            781..>989
                     /locus_tag="ACHOSM_RS26280"
                     /old_locus_tag="pgaptmp_005256"
     CDS             781..>989
                     /locus_tag="ACHOSM_RS26280"
                     /old_locus_tag="pgaptmp_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HGU4561507.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADL"
BASE COUNT          241 a          281 c          260 g          207 t
ORIGIN      
        1 tcagatatgc agaaaaatgt gactcccggc aggcgaaaag gctgccctaa ttatcctccc
       61 gaatttaaac agcagctcgt tgctgcctcc tgtgaacccg ggatatccat ctcaaaactt
      121 gctcttgaaa atggcattaa cgccaatctg ttgttcaaat ggcgacaaca atggcgcgag
      181 ggaaagctgc tattaccttc ttcagagagc ccccagctac ttcctgtgac tctcgatgca
      241 gctgccgaac agccagaatc gctcgcagag gacccggaaa ccctcagtat cagctgtgag
      301 gtaacgttcc ggcacgggac gctccgcttc aatggcaatg tcagcgaaaa gctcctgact
      361 ctgctgatac aggaactgaa gcgatgatcc cgttaccttc cgggaccaaa atttggctgg
      421 ttgccggtat caccgatatg agaaatggct tcaacggcct ggctgcgaaa gtacaaacgg
      481 cgctgaaaga cgatcccatg tccggccatg ttttcatttt ccggggccgc agcggcagtc
      541 aggttaaact gctgtggtcc accggtgacg gactgtgcct cctgaccaaa cggctggagc
      601 gtgggcgctt cgcctggccg tcagcccgtg atggcaaagt gttccttacg caggcgcagc
      661 tggcgatgct gctggaaggt atcgactggc gacagcctaa gcggctgctg acctccctga
      721 ccatgctgta aatctcttta tcctggttgt cacagaataa gcccggtaaa atacgggctt
      781 atgaacgaca tctcttctga cgacatcttc ctgctgaaac agcgcctggc cgaacaggaa
      841 gcgctgatcc acgccctgca ggaaaagctg agcaaccggg agcgcgaaat agaccatctg
      901 caggcgcagc tggataaact ccgccggatg aacttcggca gtcgttccga aaaagtctcc
      961 cgccgtatcg cacaaatgga agccgatct
//
LOCUS       DAZKHC020000369          960 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZKHC020000369 DAZKHC020000000
VERSION     DAZKHC020000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.375"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(173..745)
                     /gene="lpfA-O113"
                     /locus_tag="ACHOSM_RS26285"
                     /old_locus_tag="pgaptmp_005257"
     CDS             complement(173..745)
                     /gene="lpfA-O113"
                     /locus_tag="ACHOSM_RS26285"
                     /old_locus_tag="pgaptmp_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HGU4561508.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          304 a          207 c          179 g          270 t
ORIGIN      
        1 ccaatgacaa cgcctgcata agttaatgct ggttggccta aaaaaacgct gagtataatc
       61 gcggtaaata acttacatga aactgttgtt ttaaagagcc tgattaaaca actttttctg
      121 gacataacga ctccctggcc aaagccaact gaataaaaag gcccttgata aattatttct
      181 tatattcgac agtaaattgt gaatcagcgt tcgccgtacc agccgttacg gtcgcattgg
      241 ttgccacata gcgtgcaata aacttcattt cagccttgcc ttcagcatcg atagcggtat
      301 aagcagagtc gttataaagt ttaacctgcg aattatcgga ctggttaaaa atacgaatac
      361 caacgccggt tgccgcaatc gcttgtcctg taccggtaaa atcaccaggg ttgcctgctg
      421 taagcggcgt acccactttc aggtcgccgt cgccgccagg cgcttctgta ccatcaaaac
      481 gaacggctgc ttgagtgtac gttgccgggc aattttcaag tttaatactg aacttctgtg
      541 gcgacgccag actacctaca cctgaaaatg catttttact gactttaccc agtgggacaa
      601 tttgatcggc tgtatcggag gtaacttcac aagtagtgtc gacaatttca ccgacgaagt
      661 gaacaacacc atcttctgcc agtgcatgcc cggccagcat tagagatgcc agagtgaatg
      721 caccgcctat aatattacgc ttcataaaaa ctccattgat ataatatatc ctttaccatg
      781 aatcatcact tatattaata agtgtatttt tgattcaaag gaatagtaca ccaaagagac
      841 ggctttatct aaaaataata ttaattacgc aaaaataaaa tataaattta ctgtttaaat
      901 attatttaaa cagtaaaaaa tcaagtttgt tatcaggcat taatgataat tcagaatata
//
LOCUS       DAZKHC020000370          955 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZKHC020000370 DAZKHC020000000
VERSION     DAZKHC020000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.376"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(585..767)
                     /locus_tag="ACHOSM_RS26290"
                     /old_locus_tag="pgaptmp_005258"
     CDS             complement(585..767)
                     /locus_tag="ACHOSM_RS26290"
                     /old_locus_tag="pgaptmp_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HGU4561509.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            complement(764..>955)
                     /locus_tag="ACHOSM_RS26295"
                     /old_locus_tag="pgaptmp_005259"
     CDS             complement(764..>955)
                     /locus_tag="ACHOSM_RS26295"
                     /old_locus_tag="pgaptmp_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HGU4561510.1"
                     /translation="DEVRIITDGRINFIEPSTGLERKGNSKGSMLLIWRPFISPRRMF
                     TTVSKAALMAIGQGVRRAA"
BASE COUNT          283 a          221 c          168 g          283 t
ORIGIN      
        1 agcatgggta acgaagttaa tgaaaaaata attgcagtta tagtcaagac gttagcatat
       61 agagagtcta tgttgtgtta gactttaact ctacattgat ataacattag ccaaatattc
      121 aacagtgtat gcgaccaaac accaacatgt cgcatacata tacaatttga atatttaatt
      181 atatttactt aatgtatttc tataaaagca aattacaaac tcacaacaaa aaacctcata
      241 acacattaac aatcaattct tttcatcttt ataaattctc atatcaggct tgcacccgat
      301 aaaccgacga aaactattta aaacccatcg agtgaagtaa tctctaaaac caaagaaata
      361 ccaagtgaaa atattcacga taaaatgccc ggtcaaagcc cctcctgtac cgcatgcaag
      421 aacagtaaaa aatcagatgt tttcataaat atcagtcctc atcgttttgc ctggcatgtc
      481 ctttaccagc aatcttctgt atgcactaag cctagataga atccactcag tgtacactga
      541 agcccgctcg acgctttctt gttcgtaact tcgattttag tcaatcacct tgttttcctc
      601 gcacgatgtc ttagccaccg gatatcccac aggtgagccg tgtaattgaa ggtttttacg
      661 tcagattctt ttgggattgg cttgcgttta tttctggagc gtttcgttgg aaggtatttg
      721 cagttttcgc agatgatgtc ggtgaaactt cgtcgctgtc gcctcatgcc gccctcctga
      781 cgccctgccc gatcgccatc aatgccgctt tggatacggt agtaaacatc cgtcgaggac
      841 tgatgaacgg tcgccaaatc agcagcatgg agcctttgct gtttcccttc ctctccagcc
      901 ctgtcgatgg ttcgataaaa ttaatccgtc catcagtgat aatgcgaact tcgtc
//
LOCUS       DAZKHC020000371          932 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZKHC020000371 DAZKHC020000000
VERSION     DAZKHC020000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.377"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            166..>932
                     /gene="cif"
                     /locus_tag="ACHOSM_RS26300"
                     /old_locus_tag="pgaptmp_005260"
     CDS             166..>932
                     /gene="cif"
                     /locus_tag="ACHOSM_RS26300"
                     /old_locus_tag="pgaptmp_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000652081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HGU4561511.1"
                     /translation="MKDITLPPPTSASCLTGAISVNTEAVLSPMQHTSALHVRDFASL
                     CSQNLKANVLLNSDDHEVPIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGREK
                     GIVEPSCGVTANAIMKLFLDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVND
                     GALGHAYIVDIPKGENSCRPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNY
                     FSCMSQNKTDLEQIATLFDIDGNVKMLRKENIQYQKH"
BASE COUNT          285 a          190 c          208 g          249 t
ORIGIN      
        1 gctggcaatc caggccgcgt cacagaaatg gacaatgccg ctgagagact ggcgaatggc
       61 atacaggcac cagaaaaatt tcagacatga cataatgccc atgccagaag ttctttatgc
      121 acctcgcctg tatttataga taaacataga gtgaatgaat gagatatgaa agacattacc
      181 cttccccccc cgacgtccgc gtcctgtctg acaggggcca tatctgtaaa tactgaagct
      241 gtattatctc ccatgcaaca cacttcagcc ttacatgtaa gagattttgc ttccctgtgc
      301 tcacagaacc tcaaagctaa tgtattgcta aatagtgatg accacgaagt acctatacat
      361 cagaaaaatc ctgctgcaat aatgcaaaat atcgactcta acatcaaaca gatggcaaca
      421 gactggggga tgtcgattga ggaggttgag gttattatag ggcgagagaa aggcattgtg
      481 gaaccctcct gcggagttac cgctaatgct attatgaaac tattcctgga caaggatggc
      541 ttcagttact gctttgaaaa tgaacagaca ctatcgctcg agcagcttca ggagcgcctg
      601 tcctgtatgc ctgaatgtaa gagctttgta ttacgtgtta atgatggtgc gcttggtcat
      661 gcttacattg tcgatattcc caaaggagaa aactcttgtc gtcctgcatt cttgtatcag
      721 tcagatttag gagagggcgt caccagaaag ttaagatttg aggactggat gacgcataaa
      781 gcattgactc cgattttgct ggatgatatt tgtaattact tctcctgcat gtctcaaaat
      841 aagacagatt tggagcagat tgcaacgtta tttgatattg atggaaatgt taaaatgtta
      901 cgaaaggaaa atattcaata tcaaaagcat ga
//
LOCUS       DAZKHC020000372          928 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZKHC020000372 DAZKHC020000000
VERSION     DAZKHC020000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.378"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..350)
                     /locus_tag="ACHOSM_RS26305"
                     /old_locus_tag="pgaptmp_005261"
     CDS             complement(<1..350)
                     /locus_tag="ACHOSM_RS26305"
                     /old_locus_tag="pgaptmp_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4561512.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPTGGEGAVISATRDPEYYFIVVLAGQSNSMAYGEGLPLP
                     ETYDRPDPRIKQLAR"
     gene            complement(854..>928)
                     /locus_tag="ACHOSM_RS26310"
                     /old_locus_tag="pgaptmp_005262"
     CDS             complement(854..>928)
                     /locus_tag="ACHOSM_RS26310"
                     /old_locus_tag="pgaptmp_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561513.1"
                     /translation="GVELETERFEQTVREVQDLVSQNG"
BASE COUNT          223 a          266 c          215 g          224 t
ORIGIN      
        1 cgcgccagct gcttaatacg cgggtccgga cggtcatatg tctccggcag cggaaggcct
       61 tcaccatatg ccatgctgtt tgactgcccc gccagaacca caataaagta atactccggg
      121 tctctggtgg cactgattac tgcaccttct ccacctgtcg gcttcaccac aacaggtgtg
      181 ctcacatcac cttctgcgac aatcgcctga ataagtgctg cgccatcatc cgtatacgaa
      241 gaaaacggcc caccgtatgg ttgccatcct tcacgaattt tttgcgcaag tgcatcagca
      301 aggtctgacg gcgatgccgc cctgaccaca tcgtaatgtt taaatgtcat gaatcctccc
      361 ggccgggata gtgtactgaa tcagataaag agcgggctga agtccggaag ttacaggaca
      421 atggcagaag ggagacttac agcccgcaat tcgaaaaagg ccgcgcagtt gcgcagagtg
      481 attaccatgg ggtattattc gccagctgaa atattacttc acgttttgtt gtttattcct
      541 tgccgcccgc gtctcccagc gcgggctttt tttgcccata aaaaaagccc ctccggagag
      601 gggcttcgct acgtgtctgt taaccatatg catggtgccg ggtgcctccc ggtgagttca
      661 gtatcagcac ctgaacccgc acagaaagga tagagtagaa acacctgcgc tgatatgccc
      721 ctccgctcag ggggattcac catgccagtt tcttttaaca aactccccgc aaaccagaca
      781 actgtcaacc gtctgaattg tgagacattt aaaattttcg gggcgtacct gatacccggt
      841 taattctgca atatcatccg ttctgactga ctaaatcctg tacttccctg accgtctgct
      901 caaaacgttc agtctccagc tcaacgcc
//
LOCUS       DAZKHC020000373          926 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZKHC020000373 DAZKHC020000000
VERSION     DAZKHC020000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.379"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..926)
                     /locus_tag="ACHOSM_RS26315"
                     /old_locus_tag="pgaptmp_005263"
                     /pseudo
     CDS             complement(1..926)
                     /locus_tag="ACHOSM_RS26315"
                     /old_locus_tag="pgaptmp_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          198 a          293 c          239 g          196 t
ORIGIN      
        1 cgtggagtag ctcatctggg cgtaattccg ggaaagctgc tcatacgaga cacccagccc
       61 ggcagcgata taccgcaaca gtgactgctc aaacacggag tagccgttat ccgtgtcctg
      121 agccgtctgc aggttcagtg agtcccccgg catcaggtgc ggcacttttg cgcctcccag
      181 acggaccggt gctgcggcgt aatacgcggc aatttaacca atccagcccg tcagcctttc
      241 ccgctgctcc tgactgttag cgcccagaat aaaatccatc gctgactgcg tatccagctc
      301 actctcgatg gtggcggcat acatcgcctt cacaatggcg ctctgcagct gcgtgttctg
      361 cagcgtgtcg agcatcttca tctgctccat cacgctgtaa aacacatttg caccgcgggt
      421 ctgcccgtcc tccacgggtt caaagacgtg aatgaacgaa gcacgaccac cgggtaactc
      481 acggggtatc catgtccatt tctgcggcat ccagccagga tagccgtcct cgctgacgta
      541 atatcccagc gccgcaccgc tgtcattaat ctgcacaccg gcacggcagt tccggctgtc
      601 gccggtattg ttcgggttgc tgatgcgctt cgggctgacc atccggaact gtgtccggaa
      661 aagccgcgac ggactggtat cccaggtggc ctgaacgaac agttcaccgt taaaggcatg
      721 catggccaca ccttcccgaa tcatcatggt aaacgtgcgt tttcgctcaa cgtcaatgca
      781 gcaacagtca tcctcggcaa actctttcca tgccgcttca acctcgcggg aaaaggcacg
      841 ggcttcttcc tccccgatgc ccagatagcg ccagcttggg cgatgactga gccggaaaaa
      901 agacccgacg atatgatcct gatgca
//
LOCUS       DAZKHC020000374          920 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZKHC020000374 DAZKHC020000000
VERSION     DAZKHC020000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.380"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            35..316
                     /locus_tag="ACHOSM_RS26320"
                     /old_locus_tag="pgaptmp_005264"
     CDS             35..316
                     /locus_tag="ACHOSM_RS26320"
                     /old_locus_tag="pgaptmp_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HGU4561514.1"
                     /translation="MSDLFTRMCCRMDVATVRMMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFEGRTLTVTRYDTYNGKPRIFVEQE"
     gene            319..>920
                     /locus_tag="ACHOSM_RS26325"
                     /old_locus_tag="pgaptmp_005265"
     CDS             319..>920
                     /locus_tag="ACHOSM_RS26325"
                     /old_locus_tag="pgaptmp_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4561515.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRAAATTLNRVAESIIAKTASSV
                     ARELAVPRRLIRERIRLQRASADRVYAKVIINTGNLPAIKLGTASVRLSRRKRRKKGE
                     RSVTKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     TEVVKIPTAGPLAETFERERDRMYREKLPAQMMKAMTHQLR"
BASE COUNT          207 a          240 c          296 g          177 t
ORIGIN      
        1 cctgcaaaca gcgccactgt ggcggtaaaa atcaatgtct gatctgttta cgcgaatgtg
       61 ttgccggatg gacgtggcga ccgttcggat gatgggcaaa caggcggaga ttaacggcgt
      121 cgtgtacgac gtgatgccgg aggaagagtc cgcggagatg ggggcgcttt cgggcagcca
      181 gttgtcactg gtggtgtttt cagcccggta ccgtccggcc cgtcatgatg ttgttgtgtt
      241 tgagggccgc acactgacgg tgacccgtta tgacacgtac aacggtaaac cccggatttt
      301 tgtcgaacag gaatgagtat ggcaataaaa ggtctggcgc aggccatgaa aaatctggat
      361 gcaattgacc gccgtgccgt tccccgggct gccgccacga cacttaaccg tgtggcggag
      421 tccatcatcg cgaaaacggc ctcttcggtt gccagggagc tggcggttcc gcgccgtctc
      481 atccgtgagc gtatccgcct gcaacgggcc agcgcagaca gggtttatgc gaaggtcatc
      541 atcaacaccg gtaatctgcc cgccataaaa ctggggacgg ccagcgtgcg gctttcccgc
      601 agaaagcgac gaaagaaagg cgagcgttcg gtcacgaaag gcggtggcag tgtgctgatt
      661 gtggggaaaa gacggatccc ggatgccttt atcacccggc tggctaacgg acgctggcat
      721 gtgatgcagc gtatgccgtg ggcatcatcg tccaccggcg ctgacagcaa agggaggccg
      781 aaacgccacc gtctgccgac tgaagtggtg aagattccga ctgccggacc gctggcagaa
      841 acctttgaac gtgaacggga ccggatgtac cgggaaaaat taccggcgca gatgatgaaa
      901 gccatgacgc atcagttacg
//
LOCUS       DAZKHC020000375          920 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZKHC020000375 DAZKHC020000000
VERSION     DAZKHC020000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.381"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            35..316
                     /locus_tag="ACHOSM_RS26330"
                     /old_locus_tag="pgaptmp_005266"
     CDS             35..316
                     /locus_tag="ACHOSM_RS26330"
                     /old_locus_tag="pgaptmp_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HGU4561516.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            319..>920
                     /locus_tag="ACHOSM_RS26335"
                     /old_locus_tag="pgaptmp_005267"
     CDS             319..>920
                     /locus_tag="ACHOSM_RS26335"
                     /old_locus_tag="pgaptmp_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4561517.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLR"
BASE COUNT          208 a          240 c          294 g          178 t
ORIGIN      
        1 cctgcaaaca gcgccactgt ggcggtaaaa atcaatgtct gatctgttta cgcgaatgtg
       61 ttgccggatg gacgtggcga ccgttcgggt gatgggcaaa caggcggaga ttaacggcgt
      121 cgtgtacgac gtgatgccgg aggaagagtc cgcggagatg ggggcgcttt cgggcagcca
      181 gttgtcactg gtggtgtttt cagcccggta ccgtccggcc cgtcatgatg ttgttgtgtt
      241 tgcggggcgc acactgacgg tgacccgtta tgacacgtac aacggtaaac cccggatttt
      301 tgtcgaacag gaatgagtat ggcaataaaa ggtctggcgc aggccatgaa aaatctggat
      361 gcaattgatc gccgtgccgt tccccgggcc tctgccacga cactgaaccg cgtggcgggg
      421 gccattattg cgaaaacggc ctcttcagtt gccagggagc tggccgttcc ccgtcgtctt
      481 atccgtgccc gcatccggtt aagtccggca cgaccggata aggtttacgc aaaggtttac
      541 atcaataccg gcaacctgcc cgccatcaaa ctgggggagg cccgcgttcg actttcccgc
      601 agaaaacgga gaaagaaagg acagcgtgcg gccctgaaag ggggcggcag tgtgctgatt
      661 gtggggaaaa gacggatccc ggacgccttt atcacccggc tggctaacgg acgctggcat
      721 gtgatgcagc gtatgccgtg ggcatcatca tccaccggcg cggacagcaa agggaggccg
      781 aaacgccacc gtctgccgat cgaagtggtg aagattacga ctgccggacc gctggcagaa
      841 acctttgaac gtgaacggga ccggatgtac cgggaaaaat taccggcgca gatgatgaaa
      901 gccatgacgc atcagttacg
//
LOCUS       DAZKHC020000376          885 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZKHC020000376 DAZKHC020000000
VERSION     DAZKHC020000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.382"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>885
                     /locus_tag="ACHOSM_RS26340"
                     /old_locus_tag="pgaptmp_005268"
     CDS             <1..>885
                     /locus_tag="ACHOSM_RS26340"
                     /old_locus_tag="pgaptmp_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561518.1"
                     /translation="TDNVKTPDTPKWPCDNWEEQQPINSTFSGEGYNPEQFDLAQQQL
                     QQINACHTNTTYTNADYSKVVVQLVSLINNIETISSTPLTQQTQSILNQIHNIRYEKD
                     KDSVCQIIVVVNPEPNNPIITKILVEEGIPEIFSVQTVSSDNKNFAGQRADLPTDIRD
                     IQSLYLKMAKLYIEHSENENRMEALAGCDFIDFNMTGQDMSRLVLTLSNFYFEDLLNI
                     NFTDANLFNTTFSHKENPIPKLHKYEQHLDKQINGLFSTLLTINDNSLRAKAEIASRI
                     IDFLEAKVVNLSFDDILKY"
BASE COUNT          328 a          175 c          138 g          244 t
ORIGIN      
        1 accgataatg ttaaaacgcc agacacaccg aagtggcctt gtgataattg ggaagaacaa
       61 caacccatta attccacatt ctctggagaa ggatacaacc ctgaacaatt tgatttagcg
      121 caacaacaac tccaacaaat taatgcatgc cacacgaaca ccacatatac aaatgcagac
      181 tactcaaaag ttgtggttca acttgtaagt cttattaata acattgaaac gataagttcg
      241 acaccactta cgcaacagac acaatcaata cttaatcaga ttcataacat tagatacgaa
      301 aaggataagg attctgtatg tcaaatcatt gttgtcgtta atccagaacc caacaatcca
      361 atcataacta aaattctcgt agaagaagga atacccgaaa tattcagtgt ccaaaccgtt
      421 tcttccgata ataaaaattt tgctgggcaa cgagctgact taccaactga tataagagac
      481 attcaaagct tgtacttaaa aatggcgaag ctgtatattg agcacagtga aaatgaaaat
      541 aggatggaag cccttgctgg atgcgacttt attgatttca acatgacagg gcaagatatg
      601 tccaggctag tcctaacctt atcaaatttt tattttgaag atctacttaa tataaatttc
      661 actgacgcaa atttatttaa cactactttt tcacacaagg aaaatcccat ccccaaacta
      721 cataaatatg aacagcatct tgacaaacaa atcaatggct tgttttctac attgttaact
      781 attaatgaca actctttacg cgcgaaagcg gaaattgctt cgagaataat tgatttctta
      841 gaggcaaagg tagtgaatct ttcatttgat gacatattaa aatat
//
LOCUS       DAZKHC020000377          878 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZKHC020000377 DAZKHC020000000
VERSION     DAZKHC020000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.383"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..215)
                     /locus_tag="ACHOSM_RS26345"
                     /old_locus_tag="pgaptmp_005269"
     CDS             complement(<1..215)
                     /locus_tag="ACHOSM_RS26345"
                     /old_locus_tag="pgaptmp_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561519.1"
                     /translation="MPGLVSYISSTSFANEMAEMRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGLGLVYAELLNIGFNDG"
     gene            complement(256..324)
                     /locus_tag="ACHOSM_RS26350"
                     /old_locus_tag="pgaptmp_005270"
                     /pseudo
     CDS             complement(256..324)
                     /locus_tag="ACHOSM_RS26350"
                     /old_locus_tag="pgaptmp_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(419..580)
                     /locus_tag="ACHOSM_RS26355"
                     /old_locus_tag="pgaptmp_005271"
     CDS             complement(419..580)
                     /locus_tag="ACHOSM_RS26355"
                     /old_locus_tag="pgaptmp_005271"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HGU4561520.1"
                     /translation="MREATGTSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAQ
                     DYIRTQCLN"
     gene            complement(544..669)
                     /locus_tag="ACHOSM_RS26360"
                     /old_locus_tag="pgaptmp_005272"
                     /pseudo
     CDS             complement(544..669)
                     /locus_tag="ACHOSM_RS26360"
                     /old_locus_tag="pgaptmp_005272"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap.; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
     gene            complement(623..878)
                     /locus_tag="ACHOSM_RS26365"
                     /old_locus_tag="pgaptmp_005273"
                     /pseudo
     CDS             complement(623..878)
                     /locus_tag="ACHOSM_RS26365"
                     /old_locus_tag="pgaptmp_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
BASE COUNT          211 a          255 c          186 g          226 t
ORIGIN      
        1 ccatcattaa aaccaatatt caataattct gcataaacca gtcccagccc ttcactttct
       61 gctaaaaaac ggcctgtatc tggcatataa gaaactctaa ctctctcccc tcccaggaga
      121 aatcctccaa tctgcccttc cattacctgc tgacgcattt ccgccatctc attcgcgaat
      181 gaagtcgatg atatatatga tactaatcca ggcattacca tattccttca tacagataaa
      241 tatttcctct gggcttcata acaaacatct ccctgacatg acaacaaaaa ccggagccgg
      301 actccggttt tgtgaagctg tcgggtatcc ccgcacactc gcgcagagga gtctccctgt
      361 cgtgctgcgg tctctgttaa tgagggaata cagcgacgat acggcgcatc agcaaaactt
      421 agttcaggca ctgagtgcgg atatagtcct gtgccccttc cagctgcttc tgcattgtca
      481 tcaaccgttc tctgaggatg aaataatccc gttcagcggt gtctgccagt cgggggccgg
      541 ttgcattatc cacgccggag gtgccggtgg cttcacgcac ggtaccggag caggtggcgt
      601 tgatccgcag gcgcttacga ccaatccacc agcgaatcgc ttcgcaggca ccttttcgat
      661 ctcctgcatt aattcgttta taaaacgtcg acgggaaaca cttacctggg ccaatgttgt
      721 aaggacagaa tgacgcaata cccgctttct ggggttcagt cagtggcact ttgatgtttt
      781 tctccaccca tgccagcgcc ttatcacgct caatggcgtt aacccggtcg catttttcct
      841 tcgacaactt catgcccgga acgacaggtt taccatcc
//
LOCUS       DAZKHC020000378          878 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZKHC020000378 DAZKHC020000000
VERSION     DAZKHC020000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.384"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>878
                     /locus_tag="ACHOSM_RS26370"
                     /old_locus_tag="pgaptmp_005274"
     CDS             <1..>878
                     /locus_tag="ACHOSM_RS26370"
                     /old_locus_tag="pgaptmp_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4561521.1"
                     /translation="LVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETA
                     LGVEVTKAKPVTRTITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNW
                     VTEKDVTINGKTTSQYLASVILENLPERPFNIRMVRETADSTTDQLQNRTLWSSYTEI
                     IDVKQCYPNTAIVGLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGS
                     LKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQRVPDGFGGTEP
                     RMTFNAYLSQQRKAWDV"
BASE COUNT          203 a          244 c          282 g          149 t
ORIGIN      
        1 ctggtgaaca aaaccccgct gacggacacg gacggtaatc ctgtgataca tggtgtgaca
       61 gcggtctggc gcgccgggga gcaggagcag acaccacctg aaggctttga gtcctccggg
      121 gcggaaaccg cactgggcgt ggaagtgacg aaagcaaagc cggtgacgcg caccattacg
      181 tccgcgaaca ttgaccgcct gcgggtcacc ttcggggtgc agtcactgtt ggagaccacc
      241 tcaaagggcg accgtaatcc ctcttctgtc cgactgctga ttcagttgca gcgtaacggt
      301 aactgggtga cggaaaagga tgtcaccatt aacggcaaga ccacctcaca gtacctggcg
      361 tcggtgattc tggagaatct gcctgagcgg ccctttaaca tccggatggt ccgggagacg
      421 gcggacagca ccacggacca gctgcagaac agaacgctgt ggtcgtcata caccgaaatc
      481 atcgatgtga aacagtgcta cccgaacacg gccattgtgg ggctgcaggt ggatgcggag
      541 cagttcggcg gtcagcagat gacggtgaac taccatatcc gcggtcgcat catccaggtg
      601 ccgtcaaact acgacccgga aaaacgcacg tacagcggca tctgggacgg cagtctgaaa
      661 ccggcataca gcaacaaccc ggcctggtgt ctgtgggaca tgctgaccca cccgcgctac
      721 gggatgggga aacgcctggg ggccgcggac gtggacaaat gggcgctgta tgccatcggg
      781 cagtactgtg accagcgtgt tccggacggc ttcggaggga cagagccgcg gatgacgttt
      841 aatgcgtacc tgtcacagca gcgtaaggcg tgggacgt
//
LOCUS       DAZKHC020000379          877 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZKHC020000379 DAZKHC020000000
VERSION     DAZKHC020000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.385"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..140)
                     /locus_tag="ACHOSM_RS26375"
                     /old_locus_tag="pgaptmp_005275"
     CDS             complement(<1..140)
                     /locus_tag="ACHOSM_RS26375"
                     /old_locus_tag="pgaptmp_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4561522.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQG"
     gene            complement(121..534)
                     /locus_tag="ACHOSM_RS26380"
                     /old_locus_tag="pgaptmp_005276"
     CDS             complement(121..534)
                     /locus_tag="ACHOSM_RS26380"
                     /old_locus_tag="pgaptmp_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4561523.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
     gene            complement(561..>877)
                     /locus_tag="ACHOSM_RS26385"
                     /old_locus_tag="pgaptmp_005277"
     CDS             complement(561..>877)
                     /locus_tag="ACHOSM_RS26385"
                     /old_locus_tag="pgaptmp_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4561524.1"
                     /translation="QQETTKHYVVHINQRYLETASLLVAMSLWHSHSLKGTLASPETE
                     MQQIRREVMLGWPADALNQATNRVLYLSGMLDNRHDADPEQTGKAEATEPVTSKKHSK
                     AS"
BASE COUNT          193 a          271 c          207 g          206 t
ORIGIN      
        1 ccctgttcaa cagtactggc ggtttttctg gcatccgcct ccagactgga aaaatgacgg
       61 cgtacccggg ccatctgttc atcaaaccgg gccgcatcca gactcaaatc aataaccaga
      121 tcacccgctg gctgggacat atctcacacc tccggaaatc cccgctgaag ccatcattaa
      181 tgcaacatca tcctcgctga catccaccac atccgcagaa ggtgaaatat cgcgccctcc
      241 gtcccctccg aaccggacgc ctccggcaac tcctgtcgct ttctgcatca gcatttcttc
      301 ctcgtccggc atctccgtct gctcttcctc acacgctgga gcaagcagac tgaaatccgc
      361 cggatgcata tccggatcgc caaaaaacag gctgagtacg gcgtacatca gcccggaaaa
      421 atgagcgtcc agttgggtat cctgaaaata atgcgtgcag taaaaacgtc gccagtcggc
      481 atattcggtg gatgtcatcc cggcaagcat ggcgcgccag tcgggtctcc ccatctctcg
      541 cgccagtttc tggacaaagt tcagctcgcc ttcgaatgct tttttgatgt taccggctca
      601 gtcgcttctg ctttcccggt ttgttcagga tcggcatcgt gccggttatc cagcatacct
      661 gaaagataaa gcacccggtt cgttgcctga ttcagtgcat cagcaggcca tcccagcatc
      721 acttcacggc ggatctgctg catctctgtc tccggagagg ccagagtgcc tttgagggaa
      781 tgggaatgcc atagcgacat cgccacaagc agggatgccg tttccagata tcgctggtta
      841 atgtgcacga cataatgctt cgttgtctcc tgttgtt
//
LOCUS       DAZKHC020000380          876 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZKHC020000380 DAZKHC020000000
VERSION     DAZKHC020000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.386"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..396
                     /locus_tag="ACHOSM_RS26390"
                     /old_locus_tag="pgaptmp_005278"
     CDS             <1..396
                     /locus_tag="ACHOSM_RS26390"
                     /old_locus_tag="pgaptmp_005278"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGU4561525.1"
                     /translation="DVIKRWLKQSPEAQSAIMTDEIPLDDALLQLREFIDENSGEFFV
                     QVWGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGE
                     RHNALDDARYQAKYVSVIWQKLIPSQADF"
     gene            458..727
                     /locus_tag="ACHOSM_RS26395"
                     /old_locus_tag="pgaptmp_005279"
     CDS             458..727
                     /locus_tag="ACHOSM_RS26395"
                     /old_locus_tag="pgaptmp_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4561526.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            696..>876
                     /locus_tag="ACHOSM_RS26400"
                     /old_locus_tag="pgaptmp_005280"
     CDS             696..>876
                     /locus_tag="ACHOSM_RS26400"
                     /old_locus_tag="pgaptmp_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU4561527.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIA"
BASE COUNT          260 a          200 c          216 g          200 t
ORIGIN      
        1 gacgtcatta aaaggtggct gaagcaatca cctgaagccc agtctgccat tatgaccgat
       61 gaaatcccgt tagatgatgc actgttacaa ttgcgggaat ttatcgacga aaactccggt
      121 gaattttttg ttcaggtctg gggaaatgga gccaacttcg ataacacgat tttgcgccgt
      181 tcatacgaac ggcaggggat cccctgcccg tggcgttact acaacgatcg cgatgtacgc
      241 acaatcgttg agctggggaa agccatagac ttcgatgcca gaacggctat tccattcgaa
      301 ggtgagcgcc ataatgcact tgatgacgcc cgttatcagg caaaatacgt ttcagttatc
      361 tggcaaaaac tgatcccgag tcaggctgat ttttaatgtt caaccgtcgc cagttgtcgt
      421 tggtattctg caactggcgc gttccggagt gatagccatg agcgaacagt acctgataac
      481 gctcgatgag tggaagccaa aacggttcag tctcccaata acaaacacta ccctggtgaa
      541 atacggaaaa ctaggataca tcgttccaag accacaaaaa attcgtgggc gttggctgat
      601 agatcgccga gcagtatttg ttggacctgg tgaaacagga attgcgccgg aaattcatac
      661 tggcgatgat gatgcactga aggagatttt aactcatgtc accgaggcca cgaaaaaaca
      721 gcactgacgt agccggactt tacgaaaaat ttgatcgcag aactggcaga gtttactacc
      781 agtataaaaa ccctgtgact ggaaaatttc acgggctcgg aacagacaaa ggtaaggcag
      841 aaaaaatcgc ttccacagcc aatcagcgaa tagctg
//
LOCUS       DAZKHC020000381          871 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZKHC020000381 DAZKHC020000000
VERSION     DAZKHC020000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.387"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..263)
                     /locus_tag="ACHOSM_RS26405"
                     /old_locus_tag="pgaptmp_005281"
     CDS             complement(<1..263)
                     /locus_tag="ACHOSM_RS26405"
                     /old_locus_tag="pgaptmp_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HGU4561528.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAA"
     gene            complement(351..>871)
                     /locus_tag="ACHOSM_RS26410"
                     /old_locus_tag="pgaptmp_005282"
     CDS             complement(351..>871)
                     /locus_tag="ACHOSM_RS26410"
                     /old_locus_tag="pgaptmp_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HGU4561529.1"
                     /translation="DLVYKVLTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMM
                     RLQKEGDRALNIRPAFMLVPVALETVANQTIKSASVKGADANAGVINPIQNFAEVIAE
                     ARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAP
                     LDWRGLVRSSVA"
BASE COUNT          184 a          275 c          223 g          189 t
ORIGIN      
        1 gcggccaccg caccgtctgc agccagctgc acaaccccgt ccttcagata aaccaccgcg
       61 ccggactgaa tgtcgtcagc agactgtttg ggcagcagga acacgccttc ggcaaaaccg
      121 tcaccggcct caccggcagg aatatcggta atggccacgg ccaccatact gccgaccacc
      181 accgcagcac cgctcaggat ggtctgatct ccggcattca ccagttcaat ggtggtaccg
      241 tcctgtacaa aatttttcgc cataatgctg tttctccgga cagcccctgt ggggctgttt
      301 ttcaggcata aaaaaagccc tttcgggcag tgattgtgat aacgcggtta tcaggccacc
      361 gacgaacgca ccagcccgcg ccagtcaagt ggtgccactc cggcatcaat acggattttt
      421 gtggcaatgc cgtcagtggt gaaaccttcc tgctgatcaa tgtatggcgt gtccacacca
      481 tccagccagg ccacttcaat ggtgtcagtg ccctgtgccg ccgccagata ccaggttttc
      541 gggtctgccg catcaagacg cgcttctgca atcacctcag caaagttctg gatagggtta
      601 atgacaccgg cgtttgcatc cgcccctttc acactggccg atttgatggt ctggttcgcc
      661 accgtctcca gtgccaccgg taccagcata aaggccggac ggatattcag ggcacgatcg
      721 ccttctttct gcaggcgcat catctgacgg gccgcatcca gtccggaaac ggagatcccc
      781 ccggtggcaa tatttttgtg atcggcatgg aacagcgcct taccgtcgga cagtttcggg
      841 ttatccgtca gcaccttgta gaccaggtca c
//
LOCUS       DAZKHC020000382          863 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZKHC020000382 DAZKHC020000000
VERSION     DAZKHC020000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.388"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>863
                     /locus_tag="ACHOSM_RS26415"
                     /old_locus_tag="pgaptmp_005283"
     CDS             <1..>863
                     /locus_tag="ACHOSM_RS26415"
                     /old_locus_tag="pgaptmp_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4561530.1"
                     /translation="GTVEQVKLLLVQGDLALAEFIQSAWETTKNVPGVGALFGELAEE
                     NRVFIEKTKRDELALRKSIAERDARIRQGEMGYINRSRATGVSKGPGQQEAVSRLAEE
                     LTGKKHTSPKTRSAGEREEEQAREALLALEAELRTLEKHSGANEKISRQRRDLWKAES
                     QYAVLKEAATKRQLSWQEKSLLAHEKETLEYKRQLADLGDKVEHQKRLNELAQQAVRF
                     EEQQSAKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPQALARVTGALKQTWADED
                     MLRGDWLAGLKS"
BASE COUNT          227 a          205 c          294 g          137 t
ORIGIN      
        1 ggaaccgttg aacaagtgaa gctgctgctt gtgcaggggg atctggcgct ggctgaattt
       61 attcagtcag cctgggaaac gacaaagaat gtgcccggcg ttggtgcgtt gtttggtgaa
      121 ctggcagaag agaaccgcgt atttattgag aaaacaaaac gcgatgaact ggcgctgaga
      181 aaatccattg cggaacggga tgcgcgtata cgccaggggg aaatggggta catcaaccgc
      241 tcgcgtgcaa caggcgtcag caaaggtcct gggcagcagg aagccgtcag ccgtctggct
      301 gaagagctga caggtaaaaa gcatacatca ccgaaaacgc gctctgccgg ggagagggaa
      361 gaggagcagg caagagaggc tctgcttgcc cttgaagctg agctcaggac gctggaaaaa
      421 cacagcggtg cgaatgagaa aatcagtcgg cagcgccgtg atttatggaa ggcggaaagt
      481 cagtatgcgg tcctgaaaga ggctgccacg aaacggcagt tatcctggca ggaaaaatcc
      541 ctgctggccc atgagaaaga gacgctggag tacaaacgcc agctggctga cctgggcgac
      601 aaggttgaac accagaaacg cctgaatgag ctggcacagc aggcggtgcg gtttgaagag
      661 cagcagagcg cgaagcaggc cgccatcagc gcaaaagccc gcggtctcac tgaccgtcag
      721 gcgcagcggg agtctgaagc gcagcgtctt cgggacgtgt acggtgataa tccgcaggcg
      781 ctggcccggg tcaccggggc actgaaacag acatgggcgg atgaagacat gctgcgcggt
      841 gactggctgg ccgggctgaa gtc
//
LOCUS       DAZKHC020000383          861 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZKHC020000383 DAZKHC020000000
VERSION     DAZKHC020000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.389"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..861)
                     /locus_tag="ACHOSM_RS26420"
                     /old_locus_tag="pgaptmp_005284"
                     /pseudo
     CDS             complement(1..861)
                     /locus_tag="ACHOSM_RS26420"
                     /old_locus_tag="pgaptmp_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT          288 a          149 c          123 g          301 t
ORIGIN      
        1 tgctttgatc atatcatccg ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt
       61 gttattttta tcaatcagcc ctttattcat attaaacctg ttaataaaat ggatcatctg
      121 ttgggtatcg aaatccacct tgctgaatcg atgttgacgc ataaaatcat cgaacttgtt
      181 atcttcacca gaatggatat aactgcgtat aattggatct tttatataag gacaatggct
      241 aaatgttttc ttcatattct caataaagtt tttattaaca acattaagaa tttttaattg
      301 cataaccagt tttactgcca gaacggattt caatatcatt gcattatctg gcaatgtatc
      361 aatcattttt ataatgttat tgatataacc atcgagatct tcattcccaa atatatctga
      421 ataatcaata gttaattcca attgttctgt aatgcaatct ttaaaattcc ctgtcgatat
      481 tcgcacatga tcaaacataa cagctgtcat attcgattta ataaatgata tttctttggc
      541 agacagaaac tctgctctgg tgaagtgcga agcataaaga taacaattat cgaagtttga
      601 atttctaatc tgacattcgc gaaaattact ctgagtaagt tgactctgcg agaagtcaac
      661 tgtatcaaga atatttttgc tgaaatcgac acattcacca ctcgctttga taaaaatcac
      721 attgtgtaaa cgtcgacatc taaatatgac attatcaact attgcagtag taaaatttca
      781 atccccaatt tctttacatt cgaatgttac atgttgtaga tttgatgata aaaaagacac
      841 cttcgatata acgtcacctt t
//
LOCUS       DAZKHC020000384          853 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZKHC020000384 DAZKHC020000000
VERSION     DAZKHC020000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.390"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            141..>853
                     /locus_tag="ACHOSM_RS26425"
                     /old_locus_tag="pgaptmp_005285"
     CDS             141..>853
                     /locus_tag="ACHOSM_RS26425"
                     /old_locus_tag="pgaptmp_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4561531.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKA"
BASE COUNT          215 a          201 c          249 g          188 t
ORIGIN      
        1 gagcgtaatt tccagcgggg attgcagttg ctggaagcga aattaaaaga aaatgacccg
       61 ctggcctgaa ataaaaaaat aacccgagcg gcgactcagg ttatttgatt aaacaaggta
      121 tcaaatgaga ggttaataat atgactgata ttaacgatgt aatcaagacc attgatgaac
      181 ttattgatgg cggcgtactg acgcagtatg ccatcgccag agaggcggga atttccgacg
      241 gcacattatc ggctttccgt aagggaaaat ataaaggcga taacgctgct gtggctgctt
      301 ccctgcgttc ctggtatgag aactggaata aacaaagcgc actgccggaa ccgccgcagt
      361 ttgtggaaac gcagacagtc caggagctgc gcgcactgtt tcaggcggtt cgtctgatgg
      421 gctgtattaa cgttattgtg ggcgtaccgg gtgtgggtaa aacggccact gcccgtaatt
      481 actgccagga gcaaccaaac acctggatga tcaccctgtc acccgcgcac tccagcgtca
      541 cggagtgtct gctggagctg gccgatgcgc tggggattga ttacacccgc gcgaacaaag
      601 gggcattatc ccgcgccatc cgccgtcgcc tgatgggaac gcgtggactg gtgattgtgg
      661 atgaggcgga tcatcttggt attgacggtc tggagcaact ccgggcaatt caggacgcca
      721 cggggatcgg gatggtgctt attggtaacc cgcgcggatt gtttaaaggt ggacgccgcg
      781 cctttgatga tttatcgcgc ctgttcagcc gtcttgcccg tacaaaacaa cttcgcaagg
      841 ccaaaaaggc gga
//
LOCUS       DAZKHC020000385          852 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZKHC020000385 DAZKHC020000000
VERSION     DAZKHC020000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.391"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            18..>852
                     /locus_tag="ACHOSM_RS26430"
                     /old_locus_tag="pgaptmp_005286"
     CDS             18..>852
                     /locus_tag="ACHOSM_RS26430"
                     /old_locus_tag="pgaptmp_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4561532.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DG"
BASE COUNT          191 a          179 c          283 g          199 t
ORIGIN      
        1 aaactggcga ggttttaatg gcaattattg atgatgtgat cggcgtgttt tcccccgggt
       61 ggaaagcagc cagactgcgt tcaagggcgt taatcatggc ctatgaggcg gtgaaaccga
      121 cccggacaca taaagcccgg cgggaaaatc gctctgctga tcagctcagt aaatacggtg
      181 cggtttccct gcgggagcag gcccgttttc tggatatcaa tcatgacctg gtgattggtg
      241 tgtttgacaa gctggaagag cgggtgattg gtgccagggg aattattgtg gagcctcagc
      301 cattacgaaa aaacggggaa atggcggctg agctggctgc ggatatccgc cgtttgtggg
      361 ctgaatggtc cgtgagtccg gatgtgacag ggcagtatac ccgtcctgtg cttgaacgtt
      421 tactgctgcg gacctggctg cgggatggtg aagtgtttgc gcagatggtc agtggtgcgg
      481 gaaacggtct ggaacggacg gcgggagtgc cattctggct tgaggcgatg gagccggatt
      541 ttgttcccat gcgcactgat gaatccgccg gactgaatca gggggttttt cttgatgagt
      601 ggggaagacc gaaaaaatat ctggtttata aaaattatcc ggtcagcggc cggcagagtg
      661 atacgaaaga aatcgctgcc ggaaaaatga tccacctgaa gttcactcgt cgtctgcatc
      721 agacgcgagg ctcatccatg ttatcggggg tgctgatgcg gatcagtgcc cttaaggagt
      781 atgaggatgc ggaactgaca gcggcgcgta ttgctgcggc gctgggactg tatatccgta
      841 aaggtgacgg ac
//
LOCUS       DAZKHC020000386          843 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZKHC020000386 DAZKHC020000000
VERSION     DAZKHC020000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.392"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>843
                     /locus_tag="ACHOSM_RS26435"
                     /old_locus_tag="pgaptmp_005287"
     CDS             <1..>843
                     /locus_tag="ACHOSM_RS26435"
                     /old_locus_tag="pgaptmp_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HGU4561533.1"
                     /translation="LDDELAYALWPGDVDAGNIDGSIHRWAKNEVIDNDREDWKRISA
                     SMRKQPDALRYDRQTIFGLVRERPIDIHKDPIALNKYICEYLTTKGVFENEETDLGTV
                     DVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYFLFADKDGEKCGRANKLS
                     GLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQPEPIKVTADEVNKIMQA
                     ANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSVNQNQHESERNYQKAEQN
                     SPNA"
BASE COUNT          266 a          201 c          208 g          168 t
ORIGIN      
        1 actggatgac gaactggcct acgctctctg gcctggtgat gtggatgccg gaaacattga
       61 cggcagcatc catcgctggg caaaaaatga agttatcgac aacgaccgcg aagactggaa
      121 gcgtatctcg gcatcaatgc gcaaacagcc tgatgccctt cgctacgacc gccagactat
      181 ttttggcctt gtccgtgagc gtccgatcga cattcacaaa gatcccatag cactgaacaa
      241 atatatctgc gaatacctga cgacaaaggg cgtgtttgag aatgaagaaa cagacctggg
      301 cactgttgat gttctccagt catcagaaac acaaactgat gcagtggaaa ctgaggtatc
      361 tgatatccca aaaaatgaaa ccgcgccgga agctgaacca tctgtagagc gtgaggggcc
      421 gttctatttc ctcttcgcag ataaggacgg agaaaaatgc ggtcgcgcaa acaaactttc
      481 tggtctggat aaggcactgg ctgctggcgc cactgaaatc acaaaagaag aatattttgc
      541 ccgaaaaaat ggcacataca cgggcttacc gcaaaatgta gataccgctg aagattcaga
      601 acaaccagag ccgataaaag ttaccgctga cgaagtaaac aaaattatgc aggcagccaa
      661 tatcagccag cctgacgccg ataagttgct tgctgcatca cgtggtgaat ttgttgaagg
      721 gattagtgac ccgaatgatc cgaaatgggt taaggggatc cagacccgcg attctgtaaa
      781 ccagaaccag catgaatcgg aacggaacta ccaaaaagcg gaacaaaaca gcccaaatgc
      841 gtt
//
LOCUS       DAZKHC020000387          840 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZKHC020000387 DAZKHC020000000
VERSION     DAZKHC020000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.393"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            105..821
                     /locus_tag="ACHOSM_RS26440"
                     /old_locus_tag="pgaptmp_005288"
     CDS             105..821
                     /locus_tag="ACHOSM_RS26440"
                     /old_locus_tag="pgaptmp_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4561534.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          278 a          143 c          175 g          244 t
ORIGIN      
        1 atttctaata gacgagattt ttcctgttat gatataatat gctgatttaa cacatgttaa
       61 cgatttacca gtaatgtaaa taaattttcg aggagatcat tccagtggga cgtaaatggg
      121 ccaatattgt tgctaaaaaa acggctaaag acggtgcaac gtctaaaatt tatgcaaaat
      181 tcggtgtaga aatctatgct gctgctaaac aaggtgaacc cgatccagaa ttaaacacat
      241 ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt tccaaagcac gttattgata
      301 aagcaattga taaagccaaa ggcggcggag atgaaacgtt cgtgcagggc cgttatgaag
      361 gctttggtcc taatggctca atgattatcg ccgagacatt gacttcaaat gttaaccgta
      421 cgattgctaa cgttcgcaca attttcaata aaaaaggcgg caatatcgga gcggcaggtt
      481 ctgtcagcta tatgtttgac aatacgggtg tgattgtatt taaaggatca gaccctgacc
      541 atatttttga aattttactt gaagctgaag ttgatgttcg tgatgtgact gaagaagaag
      601 gtaacattgt tatttatact gaacctactg accttcataa aggaatcgcg gctctaaaag
      661 cagctggaat cactgagttc tcaacaacag aattagaaat gattgctcaa tctgaagttg
      721 agctttcccc agaagattta gaaatctttg aagggcttgt tgatgccctt gaagatgacg
      781 acgatgtaca aaaagtttat cataacgtcg caaatctcta attatctttt aaagaaatct
//
LOCUS       DAZKHC020000388          839 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZKHC020000388 DAZKHC020000000
VERSION     DAZKHC020000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.394"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>839)
                     /gene="sepL"
                     /locus_tag="ACHOSM_RS26445"
                     /old_locus_tag="pgaptmp_005289"
     CDS             complement(<1..>839)
                     /gene="sepL"
                     /locus_tag="ACHOSM_RS26445"
                     /old_locus_tag="pgaptmp_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HGU4561535.1"
                     /translation="AMITSSSLSETIEGLSLGYRKGSARKEEEGTTTEKLLNDMQELL
                     TLTDSDKLKELSLKNSGLLEQHDPNLAMFGNMPKGEIVALISSLLQSKFVKIELKKKY
                     AKLLLDLLGEDDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSEKGASLLDWFME
                     IKDLPEREKHLKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRVIIFLSLDNYTDIIA
                     ISINKDKDVILNEVLSIIEHVWLTEDWLLESPSRVSIVEDKHVYYFHLLKDFFASLPD
                     ACF"
BASE COUNT          266 a          187 c          122 g          264 t
ORIGIN      
        1 taaaacaagc gtcaggtaat gatgcaaaaa agtctttcaa taggtggaaa taataaacat
       61 gtttatcttc gacaatcgat acccgagaag ggctttccag gagccagtct tctgttaacc
      121 atacatgttc aataatagac aatacttcat ttaaaatgac atctttatct ttattaatag
      181 agattgcaat aatatctgta tagttatcaa gcgataaaaa aatgattacc cggcataaat
      241 cgctaataat tgaagatgtt ttcaccttat cttcaaaaga agacatataa gacagatcga
      301 acgatagcgc cctaataata acctttaagt gcttctcacg ctctggaaga tccttaatct
      361 ccataaacca gtcaagtaaa gaggctccct tttcagaatc ctcatccttg gccttttcat
      421 aaagcttttt gatcttctgg ataccttcct gatttaactc acccacccct aaccaggaaa
      481 gcagagccaa ctcccaatca tcttcgccta ataaatctaa taataatttg gcgtatttct
      541 tttttaattc aatcttaaca aacttagatt gtaataaaga agatataagt gcaacaatct
      601 ccccttttgg catattacca aacatagcca aattaggatc atgctgttcc agcagcccac
      661 tgttctttaa tgatagttct tttaatttat cgctatcggt cagagtaagc agctcttgca
      721 tatcattaag cagcttctcc gttgtcgtac cttcctcttc tttccttgca ctgcctttgc
      781 gatatcccag gcttagccct tcaatcgttt cagaaagcga tgaactagta atcatcgct
//
LOCUS       DAZKHC020000389          818 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZKHC020000389 DAZKHC020000000
VERSION     DAZKHC020000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.395"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..714
                     /locus_tag="ACHOSM_RS26450"
                     /old_locus_tag="pgaptmp_005290"
     CDS             <1..714
                     /locus_tag="ACHOSM_RS26450"
                     /old_locus_tag="pgaptmp_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HGU4561536.1"
                     /translation="YSLSFIKQLPNTVNLSSLTYNKEDDFLYATQNSPAQLLKIARSG
                     DIIGRAPLPFISDAETIEHIQGNIFAAVDEKTSELFFFTVTKDMHISLRNKIQLEKFN
                     KKNRGFEGLAWKAEGRMLFVAKERRPTGMFAYQLSPDLLSAKQVTIPEELNDIHVKDI
                     SGLDFNNESLMILSDESRKLLKFNLTEMSFVEMMDLTKGNHSLTSDLLQPEGIVTLPD
                     ESIYVASEPDILAKFVPNK"
BASE COUNT          274 a          167 c          150 g          227 t
ORIGIN      
        1 tactcacttt catttataaa acaattacca aatacggtga acttatcatc tcttacgtat
       61 aataaagagg atgatttcct gtatgccacc cagaatagcc ctgcgcagct acttaaaata
      121 gccagaagtg gcgacatcat cggccgagcc ccgctgcctt ttatctctga tgccgaaacc
      181 attgagcaca tacaagggaa catttttgct gcagtagacg aaaagacttc tgaactattc
      241 ttttttacag ttacaaaaga tatgcatatc tccttgcgaa ataaaattca gttagaaaaa
      301 ttcaataaaa aaaatagagg gtttgaagga ctggcatgga aagcagaagg ccggatgctg
      361 tttgtcgcta aagaacgacg ccctacaggc atgtttgcct atcagctttc tccagatctg
      421 ctgagtgcaa aacaagtaac gattccagag gaactaaatg atatacatgt taaagatata
      481 tccggcctgg actttaataa tgaaagtctg atgattctgt ccgatgaatc gcgtaagtta
      541 ctcaagttta acctgacaga aatgtctttc gttgagatga tggacctgac aaaaggaaat
      601 cattctctga ccagtgattt gctccaacct gaaggcattg taactctgcc tgatgaaagc
      661 atttatgtcg ctagtgaacc tgatatcctc gctaaatttg tccctaataa ataagtctgt
      721 cactagcctt agtattgagc gaccaaatct ggcaatctca tgaaactcgc aaaatacgaa
      781 tctaatatta ataaaaattt tactctaaaa taaacaga
//
LOCUS       DAZKHC020000390          815 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZKHC020000390 DAZKHC020000000
VERSION     DAZKHC020000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.396"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..556)
                     /gene="fimE"
                     /locus_tag="ACHOSM_RS26455"
                     /old_locus_tag="pgaptmp_005291"
     CDS             complement(<1..556)
                     /gene="fimE"
                     /locus_tag="ACHOSM_RS26455"
                     /old_locus_tag="pgaptmp_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HGU4561537.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWER"
BASE COUNT          262 a          190 c          159 g          204 t
ORIGIN      
        1 ttctttccca taatccggca aaacgagcag cattactggc ggtataacgc acagtatggc
       61 gaatatttcg atgcccgaga taatcctgaa ttaaacgggt atctgcacca cgctccgcca
      121 gttcataacc gcaagcatgc cttaacatat gaggatgagt ctgcgttacg gttccagctt
      181 caataccggc atcgcgaata atgcgatagg cctgctggcg agaaagccga ctcccgcggc
      241 gagaaataaa tatagcgtcg gtccggtcag cgcctttcca gttagcacgt tcctgggtcc
      301 agcgttccac ggcttcacgc tcatcaaaac gtaacgggtg aacggtagaa aatccgttct
      361 tcagtcggcg aatatttatt cgaccttcat taaggtcaag gtcctgataa tgcagatcaa
      421 gcagttcact aatacgcatc ccatgccgat atgccaacag aataagacaa taatctctgg
      481 ctcccgttgc cccgtaacaa accgcctgca tcatggcctg aacttcttta ccggtaagat
      541 aacgacgttt actcacaata gtagtactcc tgactgagat atatttgaat gaatacctat
      601 aggaaacctc aatcggtcaa aattagccca atagataaaa agataaccac acacgaagtg
      661 atgtggctat cagtcaatta cattaaatat catacaaaaa taaaatatca ctgatgtgat
      721 aacatctatt ttcattgatt tgattcaata taacaataga ttgcagtgga ttaacttcca
      781 gttgcttaaa aaataaagaa aaaatgtgaa aacag
//
LOCUS       DAZKHC020000391          813 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZKHC020000391 DAZKHC020000000
VERSION     DAZKHC020000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.397"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            21..311
                     /locus_tag="ACHOSM_RS26460"
                     /old_locus_tag="pgaptmp_005292"
     CDS             21..311
                     /locus_tag="ACHOSM_RS26460"
                     /old_locus_tag="pgaptmp_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HGU4561538.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATREEREGKMSTNLVFKECRQSAAMKRVLAVYGVKR"
     gene            385..>813
                     /locus_tag="ACHOSM_RS26465"
                     /old_locus_tag="pgaptmp_005293"
     CDS             385..>813
                     /locus_tag="ACHOSM_RS26465"
                     /old_locus_tag="pgaptmp_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HGU4561539.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGD"
BASE COUNT          229 a          168 c          240 g          176 t
ORIGIN      
        1 ggactgaaag gagcaagtgt atgacgggca aagaggcaat tattcattac ctggggacgc
       61 ataatagctt ctgtgcgccg gacgttgccg cgctaacagg cgcaacagta accagcataa
      121 atcaggccgc ggctaaaatg gcacgggcag gtcttctggt tatcgaaggt aaggtctggc
      181 gaacggtgta ttaccggttt gctaccaggg aagaacggga aggaaagatg agcacgaacc
      241 tggtttttaa ggagtgtcgc cagagtgccg cgatgaaacg ggtattggcg gtatatggag
      301 ttaaaagatg accatctaca ttactgagct aataacaggc ctgctggtaa tcgcaggcct
      361 ttttatttgg gggagaggga agtcatgaaa aaactaacct ttgaaattcg atctccagca
      421 catcagcaaa acgctattca cgcagtacag caaatccttc cagacccaac caaaccaatc
      481 gtagtaacca ttcaggaacg caaccgcagc ttagaccaaa acaggaagct atgggcctgc
      541 ttaggtgacg tctctcgtca ggttgaatgg catggtcgct ggctggatgc agaaagctgg
      601 aagtgtgtgt ttaccgcagc attaaagcag caggatgttg ttcctaacct tgccgggaat
      661 ggctttgtgg taataggcca gtcaaccagc aggatgcgtg taggcgaatt tgcggagcta
      721 ttagagctta tacaggcatt cggtacagag cgtggcgtta agtggtcaga cgaagcgaga
      781 ctggctctgg agtggaaagc gagatgggga gat
//
LOCUS       DAZKHC020000392          807 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZKHC020000392 DAZKHC020000000
VERSION     DAZKHC020000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.398"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..164
                     /locus_tag="ACHOSM_RS26470"
                     /old_locus_tag="pgaptmp_005294"
                     /pseudo
     CDS             1..164
                     /locus_tag="ACHOSM_RS26470"
                     /old_locus_tag="pgaptmp_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            162..392
                     /locus_tag="ACHOSM_RS26475"
                     /old_locus_tag="pgaptmp_005295"
                     /pseudo
     CDS             162..392
                     /locus_tag="ACHOSM_RS26475"
                     /old_locus_tag="pgaptmp_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233439.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(410..598)
                     /locus_tag="ACHOSM_RS26480"
                     /old_locus_tag="pgaptmp_005296"
     CDS             complement(410..598)
                     /locus_tag="ACHOSM_RS26480"
                     /old_locus_tag="pgaptmp_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561540.1"
                     /translation="MANYQLNEQLLEGCRPWIVIFDDVLTAGSHFKAMKSLILQHIPE
                     ACILGLFVARTTRGAQII"
     gene            complement(665..>807)
                     /locus_tag="ACHOSM_RS26485"
                     /old_locus_tag="pgaptmp_005297"
     CDS             complement(665..>807)
                     /locus_tag="ACHOSM_RS26485"
                     /old_locus_tag="pgaptmp_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HGU4561541.1"
                     /translation="YSLIGTCKLNDVDPESYLRHVPGVIADWPINRVSELLPWRIALP
                     AE"
BASE COUNT          209 a          174 c          214 g          210 t
ORIGIN      
        1 atttaccatt gcgaaagctt gttctggaaa agctggagat gaaatggtct ccagagcaaa
       61 tatcaggatg gttaaggcga acaaaaccac gtcaaaaaac gctgcgaata tcacctgaga
      121 caatttataa aacgctgtac tttcgtagcc gtgaagcgct acaccctccg ctggatacgc
      181 tgattcttgc catgcctgtg tcatggaaag ggacattaca gcagtatgcg ggacgtctac
      241 acagagagca taccggtaaa agcgatgtca ggatcattga ttttgtggat accgcgtatc
      301 cagtgctgct cagaatgtgg gataaacgcc agcggggtta taaggcgatg gggtacagga
      361 ttgtggctga tggtgaaggg ctttcatttt aaggagtact ttaccctttt taaataattt
      421 gagctccccg ggttgttcgg gcaacaaaga gtcccagtat acaggcttca ggaatatgtt
      481 gtagtatcag gcttttcatc gctttaaaat ggctaccggc agtaagaacg tcatcaaaaa
      541 taacaatcca gggcctgcat ccctccagta attgttcatt cagttggtag tttgccataa
      601 tcgcttctgg tgtgggccgg tttgttggta agcgtgcagc aagaaccgta ttgacgggga
      661 tgtgttattc agctggcagt gctatgcgcc acggaagcag ttcgctgacc cggtttatcg
      721 gccagtcggc tatgacgcca ggcacatggc gaaggtagct ttctggatcc acgtcattca
      781 gtttgcacgt cccgatcagg ctgtaca
//
LOCUS       DAZKHC020000393          807 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZKHC020000393 DAZKHC020000000
VERSION     DAZKHC020000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.399"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..87
                     /locus_tag="ACHOSM_RS26490"
                     /old_locus_tag="pgaptmp_005298"
                     /pseudo
     CDS             1..87
                     /locus_tag="ACHOSM_RS26490"
                     /old_locus_tag="pgaptmp_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
     gene            168..572
                     /locus_tag="ACHOSM_RS26495"
                     /old_locus_tag="pgaptmp_005299"
     CDS             168..572
                     /locus_tag="ACHOSM_RS26495"
                     /old_locus_tag="pgaptmp_005299"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4561542.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPTTSPRSQPVAAATPESLN
                     ISCEVTFRHGSLRLNGAISENILNLLIRELKR"
     gene            569..>807
                     /gene="tnpB"
                     /locus_tag="ACHOSM_RS26500"
                     /old_locus_tag="pgaptmp_005300"
     CDS             569..>807
                     /gene="tnpB"
                     /locus_tag="ACHOSM_RS26500"
                     /old_locus_tag="pgaptmp_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4561543.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPS"
BASE COUNT          210 a          218 c          207 g          172 t
ORIGIN      
        1 cgtgataagg cgctggcatg ggtggagaaa aacatcagag tgccactgac cgaaccccag
       61 aaagcgggga ttgcgtcatt ctgtccgtaa gcgtcgtctc agcaccgtct ggcagatcct
      121 gaaattcctg agagaatagt ggacaccaaa tatggtggac gctatccatg aaatcattaa
      181 ccgcagtgcg taaaaaaagc cctaattatc ccgttgagtt caaaatcaaa atggttgaac
      241 tctcgcatcg accagagatc tccgtagcgc aactcgctcg tgagcatggg atcaacgata
      301 atttgctgtt caagtggcgc cagtactggc gcgaaggaaa actacgtcct ccttcaacaa
      361 cagaaaacaa cgtgcctgag ctgctcccga taacacttga tgccgaagat gttgtcccta
      421 caacctcccc ccggtcacaa cctgtagctg ctgcgacacc tgaatcactc aatatcagct
      481 gtgaagtgac gttccggcac ggatcactcc gtctgaatgg tgccatcagc gaaaatatcc
      541 tgaacctgct gatacgggag ctcaaacgtt gatcccatta ccatcaggga caaagatctg
      601 gctggtcgct ggcatcaccg atatgagaaa cggcttcaac ggcctggcgg caaaggtgca
      661 gacgacgctg aaagacgatc cgatgtcagg tcacgttttt atcttccgtg ggcgtaatgg
      721 cagtcaggta aagctcctct ggtctaccgg cgatggactg tgtctgctga ccaaacggct
      781 ggagcgcggc cgcttcgcct ggccgtc
//
LOCUS       DAZKHC020000394          800 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZKHC020000394 DAZKHC020000000
VERSION     DAZKHC020000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.400"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..555
                     /locus_tag="ACHOSM_RS26505"
                     /old_locus_tag="pgaptmp_005301"
     CDS             <1..555
                     /locus_tag="ACHOSM_RS26505"
                     /old_locus_tag="pgaptmp_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4561544.1"
                     /translation="ALSLQVPGFRRQMNEGWYQIRIRGEDTAPEAVYARLHEPLGEGA
                     VIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMAAWGAALSAGGFSATTMLFSLG
                     ASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSR
                     RISRDISTRDEGGDGKVVVIGRQA"
BASE COUNT          187 a          191 c          262 g          160 t
ORIGIN      
        1 gcgctgtcgt tacaggtgcc gggattccgc cgtcagatga acgaaggctg gtaccagata
       61 cgtattcgcg gtgaggacac ggcaccggag gcggtgtacg cccgtcttca cgaacctctg
      121 ggtgaggggg cggtcatcca tattgtgccg cgactggccg gggccggaaa aggtggactg
      181 cagattgtgc tgggggcggc agccatcgtg ggctctttct tcacggccgg agcatcgatg
      241 gcagcctggg gtgcagcgct gagtgccggc ggtttttctg ccaccacgat gctgttttca
      301 ctgggtgcca gcatgatact gggtggtgtg gcacagatgc tggccccgaa ggcaaaaaca
      361 ccggattacc gcgcaacgga taacggcaga cagaacacgt atttttcctc actggacaac
      421 atgattgctc aggggaaccc gatgccggtg ccttacggtg aaatgctggt tggctcacgg
      481 cgaatctccc gggacatcag tacccgtgat gaaggcggtg acgggaaggt ggtggttatc
      541 gggcggcagg cataaaagcg aaaaaatccc gcagtgaccg aaggctgcgg gaacagaaaa
      601 tgaagattaa ccacaggggg ttttgttttt attggcccga aaaaactgta acgcccggga
      661 atgatatctg ccacgggggc gtacagaaaa tgtgaagaaa ttcagaaatt ttattccgtc
      721 atgacacagg caccctccgg ggtgcctgtc gtttttgggg cataaacaga ttcagacatc
      781 agacaggaga gggggacaga
//
LOCUS       DAZKHC020000395          798 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZKHC020000395 DAZKHC020000000
VERSION     DAZKHC020000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.401"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..664
                     /locus_tag="ACHOSM_RS26510"
                     /old_locus_tag="pgaptmp_005302"
     CDS             <1..664
                     /locus_tag="ACHOSM_RS26510"
                     /old_locus_tag="pgaptmp_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561545.1"
                     /translation="TPLFVAQGNQLFMNDVFLKRLFAASITSSGNPPTFSLTPEGKLT
                     ARNADISGAITANTGTLNNVTINENCVIRGKLSANQIEGDLVKTVGKAFPRNNSYASG
                     TVTVTVYDDQGFDRQIIIPPVLFRGTKHQNFNSPNQQSYWYSTCKLQVLKNGVEIFHE
                     PATDVSRVFSSVIDMPAGRGHVTLTFNVSSAGANNWTPTTYISDLLVVVMKKSTAGIS
                     IS"
BASE COUNT          199 a          195 c          222 g          182 t
ORIGIN      
        1 cacgccgtta tttgtggcgc aggggaatca gttgttcatg aacgatgtgt tcctgaagcg
       61 gctgtttgcg gcgagtatca cgtcatccgg caaccccccg acgttttccc tgacgccgga
      121 agggaagctg acagccagga acgcggatat cagcggagca attaccgcga ataccggcac
      181 gctcaataat gtcaccatta acgagaactg tgtcatcaga gggaaactgt ctgcaaacca
      241 gattgaaggc gacctggtga agacggtggg gaaagccttt ccccggaata acagttatgc
      301 cagcgggacg gtaaccgtca cagtttacga tgaccaggga ttcgaccggc agattatcat
      361 tcccccggtg ctgtttcgcg ggacgaaaca ccagaatttc aacagcccga atcagcagtc
      421 gtactggtat tccacctgta agctgcaggt gctgaagaac ggggttgaga ttttccatga
      481 accggcaacg gatgtcagcc gggtgttctc atcggtgata gatatgccgg cagggcgggg
      541 tcatgtcacc ctgacgttta atgtgtcgtc ggccggtgcg aacaactgga cgccgacaac
      601 gtacatcagt gatttactgg ttgtggtcat gaaaaaatcc acggcaggga tcagtatcag
      661 ctgacggttt attaacccgg acgggcacct caggaggtgc cttttttatt gactgaaaac
      721 aaagaggtaa tcatgcggca tttatacgca acgatattat tgtttactac cctgctggca
      781 ggaattgcct ttcctgca
//
LOCUS       DAZKHC020000396          779 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZKHC020000396 DAZKHC020000000
VERSION     DAZKHC020000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.402"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..131
                     /locus_tag="ACHOSM_RS26515"
                     /old_locus_tag="pgaptmp_005303"
                     /pseudo
     CDS             1..131
                     /locus_tag="ACHOSM_RS26515"
                     /old_locus_tag="pgaptmp_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            154..264
                     /locus_tag="ACHOSM_RS26520"
                     /old_locus_tag="pgaptmp_005304"
                     /pseudo
     CDS             154..264
                     /locus_tag="ACHOSM_RS26520"
                     /old_locus_tag="pgaptmp_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(451..651)
                     /locus_tag="ACHOSM_RS26525"
                     /old_locus_tag="pgaptmp_005305"
     CDS             complement(451..651)
                     /locus_tag="ACHOSM_RS26525"
                     /old_locus_tag="pgaptmp_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561546.1"
                     /translation="MEKNKKQPDTKGLEILSAEIINALRSGLDENTLQDRNFTTAMWI
                     AMQILCTPRCAIFAVREGQLTR"
BASE COUNT          207 a          176 c          175 g          221 t
ORIGIN      
        1 cagtgaaggt aggatcgcgc gtatatgtag tatgtttcaa atcaacagga tactacataa
       61 aagggaaacc cagcatagct ggaactatgc tgggccgatt tagacaagtt tgcatcaatg
      121 ctttacttcg ggactacata aatggcgact cagatagcgc gaactattct ggggcaatct
      181 gtaaaaaaca gcatcaatgc ttcactctga gctcgtggtg tatcctatga tattccccgg
      241 tctcctgtaa agcagagatg catctgttca ggcaggagaa aatggataaa ctgaccaccg
      301 accaaaagtt acaggctgcg cgaattgcag ctgatatcac aattgcagca atgacatcca
      361 ccagttgtgt ctttacctct tttcatgcag ccggtgattc atcagctatg caattatgga
      421 acgcgtgctt ttctgatgta attcgggcaa tcagcgagtc aactgaccct cacgaaccgc
      481 aaaaatagcg catcttgggg tacacaggat ctgcatcgct atccacattg cagtggtgaa
      541 gtttctatcc tgtaatgtat tttcatcaag tcctgaacgt agcgcattga taatttctgc
      601 gcttaatatt tccagacctt tggtatcggg ctgctttttg ttcttctcca tgattatttc
      661 ctctgactgg tatatacatc gctcatgaaa atcaaaccga caggagaagc tgtcggtttg
      721 tattgttaca tcatggcact gtcagttccg ggctgaaccg gctctggttg ctctacggg
//
LOCUS       DAZKHC020000397          770 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZKHC020000397 DAZKHC020000000
VERSION     DAZKHC020000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.403"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>770
                     /locus_tag="ACHOSM_RS26530"
                     /old_locus_tag="pgaptmp_005306"
     CDS             <1..>770
                     /locus_tag="ACHOSM_RS26530"
                     /old_locus_tag="pgaptmp_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGU4561547.1"
                     /translation="VGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTP
                     IVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTV
                     VTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKRE
                     EIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIE
                     LPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGR"
BASE COUNT          183 a          185 c          219 g          183 t
ORIGIN      
        1 gtaggcgttc cgtacatcat cgtgttcctg aacaaatgcg acatggttga tgacgaagag
       61 ctgctggaac tggttgaaat ggaagttcgt gaacttctgt ctcagtacga cttcccgggc
      121 gacgacactc cgatcgttcg tggttctgct ctgaaagcgc tggaaggcga cgcagagtgg
      181 gaagcgaaaa tcctggaact ggctggcttc ctggattctt acattccgga accagagcgt
      241 gcgattgaca agccgttcct gctgccgatc gaagacgtat tctccatctc cggtcgtggt
      301 accgttgtta ccggtcgtgt agaacgcggt atcatcaaag ttggtgaaga agttgaaatc
      361 gttggtatca aagagactca gaagtctacc tgtactggcg ttgaaatgtt ccgcaaactg
      421 ctggacgaag gccgtgctgg tgagaacgta ggtgttctgc tgcgtggtat caaacgtgaa
      481 gaaatcgaac gtggtcaggt actggctaag ccgggcacca tcaagccgca caccaagttc
      541 gaatctgaag tgtacattct gtccaaagat gaaggcggtc gtcatactcc gttcttcaaa
      601 ggttaccgtc cgcagttcta cttccgtact actgacgtga ctggtaccat cgaactgccg
      661 gaaggcgtag agatggtaat gccgggcgac aacatcaaaa tggttgttac cctgatccac
      721 ccgatcgcga tggacgacgg tctgcgtttc gcaatccgtg aaggcggccg
//
LOCUS       DAZKHC020000398          769 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZKHC020000398 DAZKHC020000000
VERSION     DAZKHC020000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.404"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(32..388)
                     /gene="grlR"
                     /locus_tag="ACHOSM_RS26535"
                     /old_locus_tag="pgaptmp_005307"
     CDS             complement(32..388)
                     /gene="grlR"
                     /locus_tag="ACHOSM_RS26535"
                     /old_locus_tag="pgaptmp_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HGU4561548.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNLITGGDIASVYQGVL
                     SEEEDIILHVHRYNHEIPSVLNIEQDYQLVIPKKVLSNDSNVTLHCHVRGNEKLFVDV
                     YARFIEPLIIKNTGIS"
     gene            585..>769
                     /locus_tag="ACHOSM_RS26540"
                     /old_locus_tag="pgaptmp_005308"
     CDS             585..>769
                     /locus_tag="ACHOSM_RS26540"
                     /old_locus_tag="pgaptmp_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HGU4561549.1"
                     /translation="MKKIILSIILTSNSCYASDCFEITGKAYNIDPLILKAIAWNESK
                     NKNGIKSKINKNGTYDIG"
BASE COUNT          277 a          136 c           97 g          259 t
ORIGIN      
        1 attcataaac aaaaattatt ttaaataaaa cttatgatat tcctgtgttt ttaataatta
       61 atggttctat aaatctggca taaacatcaa caaacaattt ttcatttcct cttacatggc
      121 aatgtaatgt gacattacta tcattactca gtactttttt agggataact aattgataat
      181 cttgttcaat gtttagcacc gagggaattt catgattata gcgatggaca tgaagtatga
      241 tgtcctcctc ttcagacagg accccctgat acacagaagc aatatctccg ccagtgatca
      301 aatttccatt tttaatcagt ataccttccc cacaggaatc ttcattgcta ataaagataa
      361 tgctatagat gccatccttc ataatcataa ctcactcagt ttcatactaa cctcactcct
      421 ttcaatttgt tctatccata agcgatatca agaataacgg agacctttct ttttctccag
      481 attgcaatgt aatatatcat taaatatact catattccaa cctgttgttt tccgtggttg
      541 acatatttaa tttctgatga atcatattga atatcaacat gaaaatgaaa aaaattatat
      601 tgagtatcat tctcacttca aattcttgtt atgcttctga ttgttttgaa attactggaa
      661 aagcatataa tatagaccca ttaatattaa aagcaattgc atggaacgaa tcaaaaaaca
      721 aaaacggaat aaaaagtaaa ataaacaaaa acggaacgta tgatatagg
//
LOCUS       DAZKHC020000399          759 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZKHC020000399 DAZKHC020000000
VERSION     DAZKHC020000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.405"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..315)
                     /locus_tag="ACHOSM_RS26545"
                     /old_locus_tag="pgaptmp_005309"
     CDS             complement(<1..315)
                     /locus_tag="ACHOSM_RS26545"
                     /old_locus_tag="pgaptmp_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin subunit A"
                     /protein_id="HGU4561550.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVN"
     gene            complement(406..482)
                     /locus_tag="ACHOSM_RS26550"
                     /old_locus_tag="pgaptmp_005310"
     tRNA            complement(406..482)
                     /locus_tag="ACHOSM_RS26550"
                     /old_locus_tag="pgaptmp_005310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(446..448),aa:Arg,seq:tct)
     gene            complement(496..572)
                     /locus_tag="ACHOSM_RS26555"
                     /old_locus_tag="pgaptmp_005311"
     tRNA            complement(496..572)
                     /locus_tag="ACHOSM_RS26555"
                     /old_locus_tag="pgaptmp_005311"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(536..538),aa:Arg,seq:tcg)
     gene            complement(582..657)
                     /locus_tag="ACHOSM_RS26560"
                     /old_locus_tag="pgaptmp_005312"
     tRNA            complement(582..657)
                     /locus_tag="ACHOSM_RS26560"
                     /old_locus_tag="pgaptmp_005312"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(622..624),aa:Ile,seq:cat)
BASE COUNT          222 a          186 c          176 g          175 t
ORIGIN      
        1 attaacgaac ccggccacat ataaattatt ttgctcaata atcagacgaa gatggtcaaa
       61 acgcgcctga tagacatcaa gccctcgtat atccacagca aaataactgc ccggtggggt
      121 gtggttaata acagacaccg atgtggtccc ctgagatata tgttcaagag gggtcgatat
      181 ctctgtccgt atactattta acgaagagac ataactttgt tgggtcgaaa agtctatcgt
      241 aaactcccgg gaataggata ccgaagaaaa acccagtaac aggcacagta cccatttaaa
      301 taatatacac ttcatataca ggtgttcctt ttggctgaag taatcagcac cagacccggc
      361 gcagatataa aaaaggcccg caaaagcgag cctggtaaat aaatatggcg cgttgtactg
      421 gattcgaacc agtgaccgat tgctcagaag gcaattgctc tgtccggctg agctaacaac
      481 gcataatgca gataatggac cgccatcgag gactcgaacc ccgcgcagcc agcttcgaag
      541 gctggcgctc tgtcccgatg agctaatggc ggtatgtgat atggtggccc ttgctggatt
      601 tgaaccagcg acctggcgat tatgagtcgc tcgctctcac cactgagcta aagggccggg
      661 cgcaggataa taacggtacg taactaatcc tgcaatatca tccgttctga ctgactaaat
      721 cctgaacttc cctgaccgtc tgctcaaaac gttcagtct
//
LOCUS       DAZKHC020000400          758 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZKHC020000400 DAZKHC020000000
VERSION     DAZKHC020000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.406"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>758)
                     /locus_tag="ACHOSM_RS26565"
                     /old_locus_tag="pgaptmp_005313"
     CDS             complement(<1..>758)
                     /locus_tag="ACHOSM_RS26565"
                     /old_locus_tag="pgaptmp_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561551.1"
                     /translation="GNDISSDLVRRKMNDLPESPIVNNLEALAPGIEKLKQTSIEMVT
                     LLNALQPGGKCVITGDFQKELAYLQNVILYNDSSLRMDFFGYNALIIQRSDNTCELTI
                     NEPLKNQEISTGNINVNCPLKDIYNEIRRLNVVFSCGTGGIVDLSSLDLRNIDLELYD
                     FTDKHMANAILNPFKLDDTDFTNANMFQVNFVSSKQNTTISWDYLLKITPVLTSISDM
                     YSEEKIKLVESCLNELGDITEEQLKIMRFAIIES"
BASE COUNT          238 a          129 c          122 g          269 t
ORIGIN      
        1 atagattcta taattgcaaa tctcatgatt tttaattgtt cttcggtaat atctccaagc
       61 tcatttaaac aactttcgac aagcttaatt ttttcttcag aatacatatc gctaatgctt
      121 gttagaacag gcgttatttt tagtaaataa tcccaggaga ttgtggtgtt ttgttttgaa
      181 ctaacgaaat ttacctgaaa catgttggca ttagtaaaat ctgtgtcatc caatttaaaa
      241 ggatttaaaa ttgcattggc catatgttta tctgtaaaat cataaagttc taaatcgata
      301 ttccgtaagt ccagagagga tagatcaact atacctccag tcccacaact aaaaactacg
      361 ttcaacctcc tgatttcatt gtaaatgtct ttcaatgggc aattaacatt gatattgcct
      421 gtggatattt cctggttttt taacggttca ttaattgtaa gttcacaggt attgtctgat
      481 cgttgaataa ttagggcgtt ataaccaaaa aaatccatac gaagagacga atcattataa
      541 agaattacat tttgtaagta cgctaattct ttttgaaaat caccagtgat aacacatttc
      601 ccacctggtt gtaacgcgtt aagtaaagtg accatttcaa tagaggtctg ttttagtttt
      661 tctatacctg gtgctagtgc ttcgaggtta ttaactatgg ggctttcagg aagatcattc
      721 atttttcgac ggactaaatc actactaata tcattacc
//
LOCUS       DAZKHC020000401          741 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZKHC020000401 DAZKHC020000000
VERSION     DAZKHC020000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.407"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..732
                     /locus_tag="ACHOSM_RS26570"
                     /old_locus_tag="pgaptmp_005314"
     CDS             <1..732
                     /locus_tag="ACHOSM_RS26570"
                     /old_locus_tag="pgaptmp_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4561552.1"
                     /translation="GIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVD
                     EAINKTYTRRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVA
                     SMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQ
                     QLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASL
                     QEEDGAATNKKTLADYARALSGEDE"
BASE COUNT          192 a          182 c          228 g          139 t
ORIGIN      
        1 ggtatcgact cccagctgga ccgctacgaa atgcgcgtat ggggatgggg gccgggtgag
       61 gaaagctggc tgattgaccg gcagattatt atgggccgcc acgacgatga acagacgctg
      121 ctgcgtgtgg atgaggccat caataaaacc tatacccgcc ggaatggtgc agaaatgtcg
      181 gtatcccgta tctgctggga tactggcggg attgacccga ccattgtgta tgaacgctcg
      241 aaaaaacatg ggctgttccg ggtgatcccc attaaagggg catccgtcta cggaaagccg
      301 gtggccagca tgccacgtaa gcgaaacaaa aacggggttt accttaccga aatcggtacg
      361 gataccgcga aagagcagat ttataaccgc ttcacactga cgccggaagg ggatgaaccg
      421 cttcccggtg ccgttcactt cccgaataac ccggatattt ttgatctgac cgaagcgcag
      481 cagctgacgg ctgaagagca ggtcgaaaaa tgggtggatg gcaggaaaaa aatactgtgg
      541 gacagcaaaa agcgacgcaa tgaggcgctc gactgcttcg tttatgcgct ggcggcgctg
      601 cgcatcagta tttcccgctg gcagctggat ctcagtgcgc tgctggcgag cctgcaggaa
      661 gaggatggtg cagcaaccaa caagaaaaca ctggcagatt acgcccgtgc cttatccgga
      721 gaggatgaat gacgcgacag g
//
LOCUS       DAZKHC020000402          735 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZKHC020000402 DAZKHC020000000
VERSION     DAZKHC020000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.408"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..180)
                     /locus_tag="ACHOSM_RS26575"
                     /old_locus_tag="pgaptmp_005315"
     CDS             complement(<1..180)
                     /locus_tag="ACHOSM_RS26575"
                     /old_locus_tag="pgaptmp_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561553.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTRLSIST
                     QYVVLLVTNIITINRH"
     gene            323..538
                     /locus_tag="ACHOSM_RS26580"
                     /old_locus_tag="pgaptmp_005316"
     CDS             323..538
                     /locus_tag="ACHOSM_RS26580"
                     /old_locus_tag="pgaptmp_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4561554.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            538..>735
                     /locus_tag="ACHOSM_RS26585"
                     /old_locus_tag="pgaptmp_005317"
     CDS             538..>735
                     /locus_tag="ACHOSM_RS26585"
                     /old_locus_tag="pgaptmp_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4561555.1"
                     /translation="MPPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIMPGKTY"
BASE COUNT          205 a          141 c          203 g          186 t
ORIGIN      
        1 atgtcgatta atcgttatga tattggttac aagaagtacc acgtattgtg ttgagataga
       61 aagcctggtg ccagaggtaa atgcagcagc ataataaaaa agagccagcg cagaagagaa
      121 cgggtaaaag agtctgcgct ggcgtgggga tattccccgt ggagaaatga tatgtaacac
      181 acatcgggaa cctttctata taaacattat cattattgtc aatcataaca gtcaggtatt
      241 atgacgttta tgcatcaggg ccatcaggaa ttaactggtg gctttttatt gttgtcagct
      301 tccggataac gggagacggg gtatgtacca gatggaaaaa atcacaacag gtgtgtcata
      361 caccacgtca gcggtgggga cgggatactg gttactgcag ctgctggaca aagtctctcc
      421 gtcccagtgg gtggcaatag gtgtgctggg gagtctgctg tttggcctgc tgacgtatct
      481 gacaaacctt tatttcaaga ttaaagaaga taagcgcaag gctgcgagag gtgaataatg
      541 cctccatcat tacgaaaagc cgttgctgct gctattggtg gtggggctgt tgccatagcg
      601 tctgtgctca tcactggtcc gagtggtgac gatggcctgg aaggtgtcag ctacatacca
      661 tacgaagata tcgttggcgt atggactgta tgtcatggac acaccggaaa agacatcatg
      721 cccggtaaaa cgtat
//
LOCUS       DAZKHC020000403          735 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZKHC020000403 DAZKHC020000000
VERSION     DAZKHC020000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.409"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..163
                     /locus_tag="ACHOSM_RS26590"
                     /old_locus_tag="pgaptmp_005318"
     CDS             <1..163
                     /locus_tag="ACHOSM_RS26590"
                     /old_locus_tag="pgaptmp_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561556.1"
                     /translation="LIAWLQDNIDCESGIIFDNDEDKTDSAALLPCIEQAREDIRTLH
                     QLQFLQQNR"
     gene            248..>735
                     /locus_tag="ACHOSM_RS26595"
                     /old_locus_tag="pgaptmp_005319"
     CDS             248..>735
                     /locus_tag="ACHOSM_RS26595"
                     /old_locus_tag="pgaptmp_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4561557.1"
                     /translation="MSGITISRPEVVNGHTDVICSTSIGHILAVRKTTLMQIDTLIRQ
                     LTEISAMTESIGGKTAPDWAMKQNFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WN"
BASE COUNT          213 a          176 c          180 g          166 t
ORIGIN      
        1 cctcattgcg tggctgcagg acaatatcga ctgcgaatcg ggaattatct ttgacaacga
       61 tgaggataaa acggattcgg cagcactgct gccctgtatt gaacaggcca gggaagatat
      121 ccgtaccctg caccagttgc agtttctgca acagaaccgg taaatattac tcaccattct
      181 tcattccact gacgccagcc tgaacagggc tggcgttttc atttatctgc aaaaaggaaa
      241 aacgattatg tcaggtatca caatctcccg tcctgaagtg gtcaacgggc atacggacgt
      301 tatctgttcc acctcaatcg gacacattct ggctgtacga aagaccacac tgatgcaaat
      361 cgacacactt atccggcaac tgacagaaat ctcagcaatg acagaaagta ttggaggtaa
      421 aaccgccccg gactgggcca tgaaacagaa ttttcgctgc ggttgctggc tgatggagaa
      481 acccgaaaca gcaatgaaag ccattacccg gaatcttgac cgcgaaatct ggcgtgacct
      541 gatgcaacgt tcagggatgc tttccttaat ggatgcacag gcccgtgata catggtaccg
      601 gtcactggag tacgataatt ttccggaaat cagtgaagcg aacattctga gcacatttga
      661 acaactacac cagaacaagg atgaggtgtt tgagcgaggg gtgatcaacg tcttcagggg
      721 actgagctgg aatta
//
LOCUS       DAZKHC020000404          723 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZKHC020000404 DAZKHC020000000
VERSION     DAZKHC020000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.410"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..397
                     /locus_tag="ACHOSM_RS26600"
                     /old_locus_tag="pgaptmp_005320"
     CDS             <1..397
                     /locus_tag="ACHOSM_RS26600"
                     /old_locus_tag="pgaptmp_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4561558.1"
                     /translation="GIWTICRGAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVE
                     RNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFSKRLNAGDRRGACEAIRWWIKDGGR
                     DCRIRSNNCYGQVSRRDQESALACWGIDR"
BASE COUNT          189 a          154 c          207 g          173 t
ORIGIN      
        1 gggtatctgg actatctgcc gaggcgccat catggtggat ggtaagcctg tgattcctgg
       61 catgaagctg tcgaaggaaa aatgcgaccg ggttaacgct atcgaacggg ataaggcgct
      121 ggcatgggtg gagcgtaata taaaagttcc actgaccgag ccacaaaaag caggtatcgc
      181 gtcattttgc ccctataaca ttggccccgg taagtgtttc ccgtcgacgt tttctaagcg
      241 gcttaatgca ggcgatcgca ggggagcgtg tgaggcgatt cgctggtgga ttaaggacgg
      301 tggcagagac tgccgtatcc gttcaaacaa ctgttacggt caggtatcaa gacgtgacca
      361 ggagagtgcg ctggcatgct ggggtatcga caggtaagca gaatattttg ctgaaaaatg
      421 acgttggcca acgcgggcag ataacacgaa atcctgcgaa ctggcaaaat gtaagtgaat
      481 aaagtcaaca agattgtttc atgaagaggc accgtaatgg tgcctttgtc atttctgcgc
      541 ttcgcacaag cgtaaataaa ccaaagaacc tttcaggatg agccctggtg gataaccggc
      601 agtggtctgg ttaaccctct ttgggctggt tattcctgtg cgcagggttc atcactaaaa
      661 ggaaataacc gatgaatatg atgaccgtgc cgtttcacgg cgattctctt tatgtggtta
      721 acc
//
LOCUS       DAZKHC020000405          722 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZKHC020000405 DAZKHC020000000
VERSION     DAZKHC020000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.411"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..191)
                     /locus_tag="ACHOSM_RS26605"
                     /old_locus_tag="pgaptmp_005321"
     CDS             complement(<1..191)
                     /locus_tag="ACHOSM_RS26605"
                     /old_locus_tag="pgaptmp_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561559.1"
                     /translation="MYLHTYQHNDRLPPQKHDKGIIMEFSVIFFWVITGLITILFLKS
                     IITDLIKNLNKTDIALYILA"
BASE COUNT          185 a          132 c          157 g          248 t
ORIGIN      
        1 gccaatatat ataacgctat gtctgttttg tttaggtttt ttattagatc ggttattatt
       61 gattttaaga ataagatggt aataagacca gtgataaccc agaaaaaaat aacggaaaat
      121 tccataatta tacctttatc gtgtttctgg ggtggtaatc tatcattgtg ctgatatgtg
      181 tgtaaataca tgcattatct gtttcttata ttctttttat gaagttactg attaatttat
      241 attagaacat cacattcata atgaaatcaa catgttcata tggttgggtt gacggcagat
      301 ttcccttttt gttaacctgt atttgcatta actgtcgctt ttgcgaccag ccaatgttcc
      361 agcagtctga gagggacgcc ttcgggggat tgccagccaa tgtttcagca atctgagaga
      421 aacgccttcg ggtgattgca atatcctcgt agcctgaaag aggatgcctt tatggtggta
      481 catccgttta gcctctggga acggatatca ctacggtgat tgaggtacgt tgatttttcc
      541 ttcggattac cgtggtgctt ccggacaacc ggactactgt gtattcccgg cagactggca
      601 taaaaacgcc tttaaaatct gcgtcttaag atattttctg actttccgca tgcgcttctt
      661 atgactgtgc gcacggcata agaccatttg aaatttggtc gcttgcgtgt gcatgtggag
      721 tt
//
LOCUS       DAZKHC020000406          717 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZKHC020000406 DAZKHC020000000
VERSION     DAZKHC020000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.412"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            120..704
                     /locus_tag="ACHOSM_RS26610"
                     /old_locus_tag="pgaptmp_005322"
     CDS             120..704
                     /locus_tag="ACHOSM_RS26610"
                     /old_locus_tag="pgaptmp_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4561560.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     VVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
BASE COUNT          206 a          148 c          147 g          216 t
ORIGIN      
        1 attttggtaa caaatatcat tgctcattaa tatctagcat ctgcttataa catttgtgtc
       61 atgaatagtc attcttcaaa ccagtagtct gactaatgga attttattca aggattttta
      121 tgtctaaaaa attaggtttt gccctgtctg gtcttatgct ggcaatggtt gcaggtactg
      181 cttctgctga tatggatggt ggtcagttaa atatcagtgg tctggttgtt gataacacct
      241 gtgaaacccg cgttgacggc ggtaataaag atggtctgat cctgctgcaa actgcaactg
      301 ttggtgaaat cgatgctggc gtgctgaatg acaccgttgg cgctaaagcc aaacctttca
      361 gcatcaccgt tgactgcagc aaagcaaatc cgaatccagg tagtacagct aaaatgacct
      421 tcggttctgt tttctttggt aatagcaaag gaacgctgaa caacgacatg tctattaaca
      481 acccgtctga cggcgtgaat atcgctctgc acaatattga tggttccact atcaaacagg
      541 ttcaaattaa caaccctggc gatgtgtata ctaaagccct ggatgcaacg acaaaatctg
      601 ttgtttatga ttttaaagcg tcttacgttc gtgccgtcgc agaccaaaca gcaactgctg
      661 gttatgtaaa aactaacact gcatacacca ttacttatca gtaagtattc accaatt
//
LOCUS       DAZKHC020000407          714 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZKHC020000407 DAZKHC020000000
VERSION     DAZKHC020000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.413"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..693
                     /locus_tag="ACHOSM_RS26615"
                     /old_locus_tag="pgaptmp_005323"
     CDS             <1..693
                     /locus_tag="ACHOSM_RS26615"
                     /old_locus_tag="pgaptmp_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein"
                     /protein_id="HGU4561561.1"
                     /translation="AMINPENGSSTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPT
                     FSLTPEGRLTARNADISGNVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVSKS
                     FPRTNSYASGTITVRISDDQKFDRQVMIPPVLFRGGKHENFNSNNQQSYWYSTCRLRV
                     TRNGQEIFNQSTTDAQGVFSSVIDMPAGQGTLTLTFTVSSSGANNWTPTTSISDLLVV
                     VMKKSTAGISIS"
BASE COUNT          200 a          168 c          184 g          162 t
ORIGIN      
        1 gcgatgataa atccggagaa cggcagcagc acgccgttat ttgtggcgca ggggaatcag
       61 ttgttcatga acgatgtgtt cctgaagcga ctgtttgcgg tgagtatcac ctcgtccggc
      121 aatcccccga cgttttccct gacgccggag ggcaggctga ccgcaagaaa tgctgatatc
      181 agcggtaacg tgaatgcgaa ttccgggacg ctcaacaacg tcacgattaa tgagaactgt
      241 cagattaagg ggaaactgtc agccaaccag attgaaggtg atattgtcaa aacggtcagc
      301 aagtctttcc cccgcacgaa cagttatgcc agtggcacca tcacggtaag aatcagtgat
      361 gatcagaaat ttgaccggca ggtcatgata ccgccagtgt tattccgcgg tggtaagcat
      421 gagaatttca acagtaataa ccaacagtca tactggtatt caacctgccg gttaagagtg
      481 acccgcaatg gtcaggagat ttttaatcag tccacgacgg atgctcaggg cgtattttcc
      541 tcagttatag atatgcctgc cggacagggg acgctgacac tgacattcac cgtatcttca
      601 tcaggagcga ataactggac accaacaacc agtatcagcg atctgctggt tgtggtgatg
      661 aaaaaatcca cagcaggtat cagtatcagc tgaattttat aacccagaac gggc
//
LOCUS       DAZKHC020000408          707 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZKHC020000408 DAZKHC020000000
VERSION     DAZKHC020000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.414"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..378
                     /locus_tag="ACHOSM_RS26620"
                     /old_locus_tag="pgaptmp_005324"
     CDS             <1..378
                     /locus_tag="ACHOSM_RS26620"
                     /old_locus_tag="pgaptmp_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
                     /protein_id="HGU4561562.1"
                     /translation="ELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQ
                     EVIEQRESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARIN
                     ASYTQLEIKHSQPKNRASGKRVS"
BASE COUNT          242 a          138 c          123 g          204 t
ORIGIN      
        1 gaactcatgg gagaaatgtc gaataataat ataaaagttc atttaaatga accagagata
       61 atatttttac atgccgatct tcagcaatac ttaagccaaa gttgcggtgc gtttgtgtgc
      121 atggcagctc aggaagtgat tgaacaaagg gaaagcaatt ctgacagcgc cccctatacg
      181 ttattaaaaa actatgctga cagatttaaa aaatattcag cagaagagca gtacgaaatt
      241 gattttcaac atcgactggt aaacagaaat tgttatttag ataaatatgg cgatgcacga
      301 atcaatgcgt cttatacaca attagaaata aaacactcac aacccaaaaa tagagcatcc
      361 ggcaaaagag tgagttaaca tcattgttaa tcaattcgac cagtcagtaa atatgctgac
      421 tggtcgaaca taatttgcta aaaagtcatc actgactgat gtcagtccca ccatctgcca
      481 gttcaatcta cgggtatttc cagcgtatcg cggcacgcga ttgctggtct gttgatgtag
      541 tggattgtag ttgctgactt ttcttaccct cagatttctg ccccacttgt caattcaatc
      601 tgccaataaa taacataatt atatttttat tcctcacaga aattcctttt taaaaatagc
      661 gaacccactg taaaaaacac tttttgtata tctctaccaa gcgtaat
//
LOCUS       DAZKHC020000409          689 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZKHC020000409 DAZKHC020000000
VERSION     DAZKHC020000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.415"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>689)
                     /locus_tag="ACHOSM_RS26625"
                     /old_locus_tag="pgaptmp_005325"
     CDS             complement(<1..>689)
                     /locus_tag="ACHOSM_RS26625"
                     /old_locus_tag="pgaptmp_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4561563.1"
                     /translation="AGIMMTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDD
                     LDDLCQVQTHIRTITHRVTDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDM
                     LREAIGGLASRPEGFVVYTTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEH
                     PPEMVESGAHLLMENLAMVNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVE
                     IGLALRSDRWAG"
BASE COUNT          145 a          219 c          180 g          145 t
ORIGIN      
        1 cccgcccagc ggtcagaacg cagggcaaga ccgatttcca cattggcgtg ttttgacatg
       61 aagccacgaa atgcttcctc accagcctcc cgggctttac ggtactcccg gtacagaaag
      121 gcctcatcca ccgaataacc gagattcggg ttaaccatgg cgaggttttc catcagcagg
      181 tgagccccgc tttccaccat ttcaggagga tgctcaaaaa tcaccggcag aaagtgcgga
      241 tcatgaattt tgccgtcacg gacatcccgg gcatactgca gtttctgtct gaacaccccg
      301 gcgggcggtt cattcgactg ggtggtcgta tacaccacaa acccttccgg acgggaggca
      361 agcccgccga tggcttcacg tagcatgtct tccgccttgt actgcttgcc aaacagccac
      421 agttcatcaa tcagtgtccc cacggacttg ataccggaca ccgtattcgg atcggctgcc
      481 accaccttca gggtggtgtc cgtcacccga tgggtgatgg tccggatatg tgtctgcacc
      541 tgacagaggt catccagatc atcgtcccgt cgtaccatat ccctggcagg gttgaaggcg
      601 ttagccgcca cctccacggt cggggccaga atggtgtagc ccgccgcctg ccgccagttc
      661 agtaacagcg ccgtcatcat gatccccgc
//
LOCUS       DAZKHC020000410          687 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZKHC020000410 DAZKHC020000000
VERSION     DAZKHC020000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.416"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..481
                     /locus_tag="ACHOSM_RS26630"
                     /old_locus_tag="pgaptmp_005326"
     CDS             <1..481
                     /locus_tag="ACHOSM_RS26630"
                     /old_locus_tag="pgaptmp_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU4561564.1"
                     /translation="LSPEFTARLVDRWRELEGATAKIPQTFSEALRLAADLEDQKAEL
                     EKQLALAAPKVEFADRVGEASGILIGNFAKVVGIGPNKLFAWMRDHKILIASGARRNV
                     PMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGRGQQWLTRKLLDNGMLKVTGEAA"
     gene            481..>687
                     /locus_tag="ACHOSM_RS26635"
                     /old_locus_tag="pgaptmp_005327"
     CDS             481..>687
                     /locus_tag="ACHOSM_RS26635"
                     /old_locus_tag="pgaptmp_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HGU4561565.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTKLALERRNKERE
                     KAEKTAEKKRRREEQKQKDKIKIRK"
BASE COUNT          223 a          148 c          183 g          133 t
ORIGIN      
        1 gttgtcgccg gaattcaccg ctcgccttgt tgaccgctgg cgagaactcg aaggggcaac
       61 cgcgaaaata ccacaaacct tttctgaggc attgcgcctt gcggccgacc ttgaagacca
      121 gaaggctgaa ctggagaaac agcttgctct cgcagcacct aaagttgagt ttgccgatcg
      181 agttggcgag gccagcggaa ttttgattgg aaactttgca aaggttgttg gtattggtcc
      241 aaacaaactg tttgcgtgga tgcgcgatca caaaatcctt attgcttcag gtgcccggcg
      301 caatgtgcca atgcaggaat atatggatcg cggctatttc acagtgaaag aaacagcggt
      361 caatacaaat cacggaatac agatatcgtt caccacaaaa atcaccgggc gtggtcaaca
      421 gtggctgaca agaaagctgc tagataacgg aatgcttaaa gtaacagggg aggctgctta
      481 atggctaaac cagcgcgaag gaaatgcaaa atctgtaagg aatggtttca cccggcattc
      541 tcaaatcagt ggtggtgcag cccggaacac ggaactaaat tagcgctcga acgacgaaat
      601 aaagaacgcg aaaaggcgga aaaaacagca gagaagaaac gacgacgaga ggagcagaaa
      661 cagaaagata aaattaagat tcgaaaa
//
LOCUS       DAZKHC020000411          683 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZKHC020000411 DAZKHC020000000
VERSION     DAZKHC020000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.417"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>683
                     /locus_tag="ACHOSM_RS26640"
                     /old_locus_tag="pgaptmp_005328"
     CDS             <1..>683
                     /locus_tag="ACHOSM_RS26640"
                     /old_locus_tag="pgaptmp_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4561566.1"
                     /translation="QAAHALAIFKQLRIVDAPGSPTFGESCAQWVFDLVAALFGSYDA
                     QTGVRHIKEVFILIPKKNSKSTLAAGIMMTALLLNWRQAAGYTILAPTVEVAANAFNP
                     AGDMVRRDDDLDDLCQVQTHIRTITHRVTDTTLKVVAADPNTVSGIKSVGTLIDELWL
                     FGKQYKAEDMLREAIGGLASRPEGFVVYTTTQSNEPPAGVFRQKLQYARDVRDGKIHD
                     PHFLPVIFE"
BASE COUNT          146 a          183 c          210 g          144 t
ORIGIN      
        1 caggccgcac atgccctggc gatttttaaa caactgcgga ttgtggatgc accgggcagc
       61 ccgacgttcg gggagtcctg cgcacagtgg gtgtttgacc tggtggcggc cctgtttggc
      121 tcctacgatg cgcagaccgg tgtacgccat atcaaggaag tttttatcct tatccccaag
      181 aaaaacagca agtccacgct ggctgccggg atcatgatga cggcgctgtt actgaactgg
      241 cggcaggcgg cgggctacac cattctggcc ccgaccgtgg aggtggcggc taacgccttc
      301 aaccctgccg gggatatggt acgacgggac gatgatctgg atgacctctg tcaggtgcag
      361 acacatatcc ggaccatcac ccacagggtg acggacacca cccttaaggt ggtggctgcc
      421 gatccgaata cggtatccgg tatcaagtcc gtggggacgc tgattgatga actgtggtta
      481 tttggcaagc agtacaaagc ggaggacatg ttacgtgaag ccataggcgg ccttgcctcc
      541 cgcccggaag ggtttgtggt gtatacgacc acccagtcga atgagccgcc agccggggtg
      601 ttcagacaga aactgcagta cgcccgggat gtgcgcgacg gtaaaattca tgatccacat
      661 tttctgccgg tgatttttga gca
//
LOCUS       DAZKHC020000412          680 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZKHC020000412 DAZKHC020000000
VERSION     DAZKHC020000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.418"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            68..214
                     /locus_tag="ACHOSM_RS26645"
                     /old_locus_tag="pgaptmp_005329"
                     /pseudo
     CDS             68..214
                     /locus_tag="ACHOSM_RS26645"
                     /old_locus_tag="pgaptmp_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
BASE COUNT          205 a          119 c          131 g          225 t
ORIGIN      
        1 tcgttaatga ttggtcttgc tcattgttaa taacaaaatg ttgaatttac cgattaggaa
       61 actttttcct acgcgtattg cctccagcta tggcatgaac tttgagtttc tgccagaact
      121 gaagtggggc ttcggctacc caggcgcgat tatctttatg atcctcgcgg gcctggcacc
      181 gtatctgtac tttaagcgca agaactggct gtaactatac gatgtcgctg tgagtaagtt
      241 agtgtgctga cagcgacatt ttagccgagg gaatcgcaga taacataatt gttgtgaata
      301 atatccatgc cacgttgagt tggacgctta atgactcaca tctcacccct tcaattgctg
      361 cagagataat agaaaaaagt gtcaagccca atgaaatcaa tacaaagtag attgccttac
      421 atccagtatc tcttattctt tttgctccaa tattgatatt tacataggta ttattaataa
      481 ccagaataaa actcaaagcc aggttgatat cattcaaagt tattaatatt gaccatgatg
      541 ttaatgtata gattatgaac tgaaagcgtg ataaacgctc aagcctggat gggtagaata
      601 tatttctgat ggatgatttg aacattttta cattgtttta tatatataat tgttttgaat
      661 atatattacc tgaattatgt
//
LOCUS       DAZKHC020000413          679 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZKHC020000413 DAZKHC020000000
VERSION     DAZKHC020000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.419"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..182)
                     /locus_tag="ACHOSM_RS26650"
                     /old_locus_tag="pgaptmp_005330"
     CDS             complement(<1..182)
                     /locus_tag="ACHOSM_RS26650"
                     /old_locus_tag="pgaptmp_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4561567.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAV"
     gene            317..679
                     /locus_tag="ACHOSM_RS26655"
                     /old_locus_tag="pgaptmp_005331"
                     /pseudo
     CDS             317..679
                     /locus_tag="ACHOSM_RS26655"
                     /old_locus_tag="pgaptmp_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
BASE COUNT          177 a          136 c          160 g          206 t
ORIGIN      
        1 acagcagtaa gacttccttc ctggtattgc ttacgccaga gaaataactg gctggctgct
       61 acaccatgtt gccgggcaac gagggagacc gtcatccccg gttcaaagct ctgctgaaca
      121 attgcgatct tttcctgtgt ggtacgccgt ctgcgtttct ccggccctaa gacatcaatc
      181 atctgttctc caatgattag tctaaaaact agtattaaga ctatcactta tttaagtgat
      241 actggttgtc tggagattca gggggccagt ctagctattt aagtctggag tttaaattaa
      301 aatagggagt tttataatgc cgttaaattc ggagattaga tcaagctcat ttttaatgga
      361 ttgaatgtcc ttcgagctca agtagcatct agcggtcgag gggagtttac attaggtaat
      421 gagactgtca gcattgtatt taatgaaacc gatgggcgtt ttctatccag cggcagtagt
      481 gggggattgc ttactgagtt attcctttat gggtttaata acggccctga agctcttcgc
      541 gataggatgc tcagtatgct ttcggactca ggtgaagcac aatcgcaaga gagtattcag
      601 gacaaaatat ctcaatgtaa gtttcctgtt agttcaggaa atttccagtg cccgccagag
      661 tctattcagt gtccaatta
//
LOCUS       DAZKHC020000414          678 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZKHC020000414 DAZKHC020000000
VERSION     DAZKHC020000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.420"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>678)
                     /locus_tag="ACHOSM_RS26660"
                     /old_locus_tag="pgaptmp_005332"
     CDS             complement(<1..>678)
                     /locus_tag="ACHOSM_RS26660"
                     /old_locus_tag="pgaptmp_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4561568.1"
                     /translation="KFASYVNSVFSKIDFYKQCGVINPQNPNTAYFGDTDGRVGAVLY
                     ALLVSGHIGIREKGWSLLCELLKHEDMASFAYENKKLEKLFTLLDTRDMILNELHQHV
                     FLKGDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMESNKDSRINKNDVVLAGN
                     YEINFNGNYTARLANHKLSAGDTYNLIKTLDVCNYDSERQVLTSHHGIIRDEENACYC
                     LGALQVPF"
BASE COUNT          220 a          136 c          100 g          222 t
ORIGIN      
        1 aaatggaacc tgcaaagcac cgagacaata acatgcattt tcttcatctc ttattattcc
       61 gtgatgactg gttaaaactt gcctttctga gtcataatta caaacatcga gcgttttaat
      121 taagttatac gtatcaccgg cagagagttt atgatttgct agtcgtgcag tatagttacc
      181 attgaaattg atttcataat ttccagccag aacgacgtca tttttattta tacgactatc
      241 tttattactc tccatattcc gcatagaatt tagcaaagta aggatatatt tatcaccaaa
      301 aatagtactg aatctatcgc ctgtatgatc cccaagaaag atacaagggg taatcgcatc
      361 gccttttaaa aaaacatgtt gatgcaactc attcaagatc atgtctctcg tatctaataa
      421 agtaaagagt ttttccaatt ttttattttc atatgcgaat gaagccatgt cttcgtgttt
      481 taataattca cataataaac tccacccctt ttccctgata cctatatgtc ctgaaacgag
      541 caatgcataa agtacagcgc caactcttcc atctgtatcg ccaaaataag ccgtgttggg
      601 attttgtggg ttaatcacac cacactgttt ataaaaatct atttttgaaa acacagaatt
      661 aacatagcta gcaaactt
//
LOCUS       DAZKHC020000415          664 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZKHC020000415 DAZKHC020000000
VERSION     DAZKHC020000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.421"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..557)
                     /locus_tag="ACHOSM_RS26665"
                     /old_locus_tag="pgaptmp_005333"
     CDS             complement(<1..557)
                     /locus_tag="ACHOSM_RS26665"
                     /old_locus_tag="pgaptmp_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4561569.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMRVFHGGRVLVESEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
     gene            complement(559..>664)
                     /locus_tag="ACHOSM_RS26670"
                     /old_locus_tag="pgaptmp_005334"
     CDS             complement(559..>664)
                     /locus_tag="ACHOSM_RS26670"
                     /old_locus_tag="pgaptmp_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561570.1"
                     /translation="ALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
BASE COUNT          162 a          184 c          178 g          140 t
ORIGIN      
        1 cgacgcgata ctgcctcagg taatgcgtcc accacctgat tgcagactgc ccaccaggat
       61 aattcagcca gcgataattc ccgttcctgt atgccattca ttgcgtggcg gatgacgtca
      121 atcatccatg ctgacaggtt ttgttgagca agttgctcaa gtgattcgga tgtctggtcg
      181 cgcagctggt tgtcgcagtg ccagcacaac accatcgcgc cggtaccgta acgatgtatg
      241 acagtttcgc tgtgatgata atcaccatga ggccactggc aggatttaac gtgacgtaat
      301 aaccagtcag acagtgcacc agcgccacca gcagcacgaa tcacacgctc attgctgaaa
      361 aatggcagta atgatttatc ttccgccagc ggctggcgaa cggcaggaac gaccccggac
      421 ggcagattac gcatgctttt cggttcagac tccaccagca ccctgccgcc atgaaatacc
      481 cgcatggatt cacggcctgg cttaacgata accagaccga gttccggtac cagaacaggt
      541 cgaagtaata cccgcacatt acctccagat ccgttgctgg aatgtgcggg acggacgcgg
      601 tggccgttcg gagtaaggga gcctgacgga gattatccag tgacggtagt cgaggctaag
      661 ggct
//
LOCUS       DAZKHC020000416          663 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZKHC020000416 DAZKHC020000000
VERSION     DAZKHC020000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.422"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..288
                     /locus_tag="ACHOSM_RS26675"
                     /old_locus_tag="pgaptmp_005335"
     CDS             <1..288
                     /locus_tag="ACHOSM_RS26675"
                     /old_locus_tag="pgaptmp_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4561571.1"
                     /translation="EVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGYERLP
                     STEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            432..507
                     /locus_tag="ACHOSM_RS26680"
                     /old_locus_tag="pgaptmp_005336"
     tRNA            432..507
                     /locus_tag="ACHOSM_RS26680"
                     /old_locus_tag="pgaptmp_005336"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:465..467,aa:Ile,seq:cat)
BASE COUNT          164 a          144 c          179 g          176 t
ORIGIN      
        1 gaggtttcca ccgcctgtaa agattgtcgt gggcgcggtg ttgccattca tcgtgaagag
       61 tcggtaaaac gtggtatgcc tgttatcaga gactgccagc gttgtggtgg tcgtggctat
      121 gaaagattac cttcaacgga ggcatttaat gccatatgta atgtgaccga tgccatatct
      181 cttgatacat ggaaaaaaac agttaaacgt ttttacgata cgctggtggt gcagtttgat
      241 attgaagaag catgggcaga acaacaactg aaaaaggtga ccagatagct ttgttgattt
      301 ttcccgaatc tgtggtaaat ttgccctaac gatgggcgtt ttatgcctga cgttagaaga
      361 atttctacaa cccgccgccg agcgggtttt ttattgcgga attaattacg gaccgttatt
      421 atccggctcc cggcccttta gctcagtggt gagagcgagc gactcataat cgccaggtcg
      481 ctggttcaaa tccagcaagg gccaccatca caaaccgcca ttagcttatc aggaagagca
      541 gacgacacca taacagggtt gttggtgcgg gggcgggtcc ccgatggcgg tccattatcg
      601 gtattctgcg ttgttagctc agccggacag agcaattgcc ttctaagcaa tcggtcagtg
      661 gtt
//
LOCUS       DAZKHC020000417          656 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZKHC020000417 DAZKHC020000000
VERSION     DAZKHC020000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.423"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            37..>656
                     /locus_tag="ACHOSM_RS26685"
                     /old_locus_tag="pgaptmp_005337"
     CDS             37..>656
                     /locus_tag="ACHOSM_RS26685"
                     /old_locus_tag="pgaptmp_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGU4561572.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVP"
BASE COUNT          172 a          158 c          169 g          157 t
ORIGIN      
        1 tttttattgc cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat
       61 ttccgctcag aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag
      121 tcaaaacgat ttccgctgca cgaaatgcgc gatgatgtcg catttcagat tatcaatgat
      181 gaattatatc ttgatggcaa cgctcgtcag aacctggcca ctttctgcca gacctgggac
      241 gacgaaaacg tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa
      301 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
      361 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      421 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      481 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      541 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      601 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgcc
//
LOCUS       DAZKHC020000418          650 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZKHC020000418 DAZKHC020000000
VERSION     DAZKHC020000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.424"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(279..389)
                     /locus_tag="ACHOSM_RS26690"
                     /old_locus_tag="pgaptmp_005338"
                     /pseudo
     CDS             complement(279..389)
                     /locus_tag="ACHOSM_RS26690"
                     /old_locus_tag="pgaptmp_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031430.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          211 a          139 c           98 g          202 t
ORIGIN      
        1 atgcattaaa aaaatatcaa caactacgtt attccgcaac aacatacttt ttttaccatt
       61 tttccacttt aacaacattt tgctccactt ttccacgacc aaacaactta aaatctggtt
      121 gaaataacat acaacactat tattcgtctt cctcgagtcc gcccggagtt cgaaaaacaa
      181 accgagttaa agccattttt caaaaaatcg atttttggtt acaccaaaat tacgggactg
      241 catccgaatt cgtttatttt cgaacgtata catacaaata tacatgcaat tacaacatcc
      301 ggataactca tagaaatggt gctagtgagc atagttttta cacggacctg atccaaggat
      361 gagtcatcag tgtattgatc tttatgcatt ttgtcgctcc atgcgtttgc tcttcacttg
      421 cggctaaaaa tataacgtca aatcactcag catgaaagtg tgaaagtttc agcgcgtttc
      481 ccttacataa tctgtcgttt gtattctctc caggcatacc ggaaagacta ggtattgtca
      541 cacaaccaat accaccgact atcgagattt atatctattc ggtaatgttt aaatataaca
      601 aaaccccgta aaaacgaggt ttatgggtaa attgtattgt tgaatagcat
//
LOCUS       DAZKHC020000419          648 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZKHC020000419 DAZKHC020000000
VERSION     DAZKHC020000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.425"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            70..426
                     /locus_tag="ACHOSM_RS26695"
                     /old_locus_tag="pgaptmp_005339"
     CDS             70..426
                     /locus_tag="ACHOSM_RS26695"
                     /old_locus_tag="pgaptmp_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4561573.1"
                     /translation="MRIEFVLLYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRAQ
                     PVSGGRLGGEDLPHNA"
     gene            442..>648
                     /locus_tag="ACHOSM_RS26700"
                     /old_locus_tag="pgaptmp_005340"
     CDS             442..>648
                     /locus_tag="ACHOSM_RS26700"
                     /old_locus_tag="pgaptmp_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4561574.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATEL"
BASE COUNT          158 a          142 c          187 g          161 t
ORIGIN      
        1 gttgatttaa tttttcgttt tcttgatcac gcctttgcaa ccggcgtgct cgggtaaaag
       61 aggttactga tgcgtataga gtttgttttg ctttacccgc cgacggtgaa cacctactgg
      121 cgacgtcgtg gcagcacata ttttgtatca aaagccggtg agcgttatcg ccgggctgtg
      181 gcgcttattg ttcgccagca gcggctgaaa ttaagcctgt ccggaaggct ggcgataaag
      241 attattgccg agccaccgga taagcgccgc cgtgacctgg acaatattct gaaagcgccg
      301 ctggatgcgc tgacgcatgc ggggttgcta atggacgatg agcagtttga tgaaatcaat
      361 atcgttcgtg ctcagccagt atctggtgga cgtctggggg gtgaagattt accccataat
      421 gcttgaaggg caggtcaaaa aatgaaactg gaagatttac cgaaatacta ctccccaaaa
      481 tcccccggcc tgactgatgc atcggcctca acgtcgaaag atacgctgag tatcactgat
      541 gtgatggccg cgcagggcat gacacagaat tgggctgaga tggggttttc tgcgttcctt
      601 gggaaaatgg gcattagtat gaatgacaga gagcgggcaa cagaattg
//
LOCUS       DAZKHC020000420          632 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZKHC020000420 DAZKHC020000000
VERSION     DAZKHC020000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.426"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..138
                     /locus_tag="ACHOSM_RS26705"
                     /old_locus_tag="pgaptmp_005341"
     CDS             <1..138
                     /locus_tag="ACHOSM_RS26705"
                     /old_locus_tag="pgaptmp_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HGU4561575.1"
                     /translation="FFLSLKEDSVLLNIAFPADKVNITEFINLMENGYLLKNEVISLL
                     S"
     gene            complement(357..>632)
                     /locus_tag="ACHOSM_RS26710"
                     /old_locus_tag="pgaptmp_005342"
     CDS             complement(357..>632)
                     /locus_tag="ACHOSM_RS26710"
                     /old_locus_tag="pgaptmp_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspH"
                     /protein_id="HGU4561576.1"
                     /translation="NYVYKVQKMTQESGCLSRLFSFLSGGVTKALESKLNERHITPLS
                     PTWFPRTPLEGVLTERGLSSLLRRVQSTENLDYSNAMISNSFSNSVV"
BASE COUNT          216 a          123 c          103 g          190 t
ORIGIN      
        1 ttttttctat cattaaaaga agatagcgta cttttgaata ttgcattccc tgcagataag
       61 gttaacatta cagagtttat caatttaatg gaaaacggtt atttattaaa gaatgaagta
      121 attagtttat tgtcgtaaga tgttttatcc tgataaatta tgagctggaa ggaattcatc
      181 cattcagctc aatcccctcc tcatcacaga atctctgact tctgccagcc aaactcactc
      241 tgaatattca tcccatattt tcctaaaaac aatgtattat cgacataaaa caaacagaaa
      301 aactccagaa tcacaattat tctggagtta aaccaggagg aaagctcaaa tgataatcac
      361 actacggagt tagagaaaga gtttgatatc atagcattac tataatcaag gttttcagta
      421 gactgtacgc gacgtaacaa agaagataat cctctttccg ttaagacccc ctccagtgga
      481 gttcgaggaa accaggttgg agaaagtgga gtgatatgcc tctcatttag tttactttcc
      541 aaagccttag tcacaccgcc agaaagaaag ctaaataatc ttgagagaca cccactttcc
      601 tgagtcatct tttgtacttt gtacacataa tt
//
LOCUS       DAZKHC020000421          631 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZKHC020000421 DAZKHC020000000
VERSION     DAZKHC020000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.427"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..178)
                     /locus_tag="ACHOSM_RS26715"
                     /old_locus_tag="pgaptmp_005343"
                     /pseudo
     CDS             complement(1..178)
                     /locus_tag="ACHOSM_RS26715"
                     /old_locus_tag="pgaptmp_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558222.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(269..433)
                     /locus_tag="ACHOSM_RS26720"
                     /old_locus_tag="pgaptmp_005344"
                     /pseudo
     CDS             complement(269..433)
                     /locus_tag="ACHOSM_RS26720"
                     /old_locus_tag="pgaptmp_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="non-LEE-encoded effector NleG"
     gene            complement(545..>631)
                     /locus_tag="ACHOSM_RS26725"
                     /old_locus_tag="pgaptmp_005345"
     CDS             complement(545..>631)
                     /locus_tag="ACHOSM_RS26725"
                     /old_locus_tag="pgaptmp_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HGU4561577.1"
                     /translation="DKKQIQCVVRPLQILRADGRWENIGGMK"
BASE COUNT          167 a          171 c          124 g          169 t
ORIGIN      
        1 acagcagtaa gacttccttc ctggtattgc ttacgccaga gaaataactg gctgccacac
       61 catgttgccg ggcaacgagg gagaccgtca tccccggttc aaagctctgt tgaacaattg
      121 cgatcttttc ctgtgtggta cgccgtctgc gtttctccgg ccctaagaca tcaatcatct
      181 gttctccaat gactagtcta aaaactagta ttaagactat cacttattta agtgatactg
      241 gttgtctgga gattcagggg gccagtctac ctccactcgc cagaaagcga ccatccgttt
      301 cactatatgt gatactaacc gtctgtccgc ctactgtaat ttctccacca ccactggcgg
      361 ccatttgagc acgaagaacc tgcattccgg tggaaaatga ggatgattga atacctgaaa
      421 ctaatgatgg catgacaaaa ctccctattt aaatattcat tccaaacaca gggagtcata
      481 acaaacatct ccctgacatg acaacaaaaa ccggagccgg actccggttt tgtgaagctg
      541 tcggctattt cattccgcca atattttccc acctcccgtc agcacgcaga atttgcagcg
      601 gtcttaccac acactgtatc tgctttttat c
//
LOCUS       DAZKHC020000422          626 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZKHC020000422 DAZKHC020000000
VERSION     DAZKHC020000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.428"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>626
                     /locus_tag="ACHOSM_RS26730"
                     /old_locus_tag="pgaptmp_005346"
     CDS             <1..>626
                     /locus_tag="ACHOSM_RS26730"
                     /old_locus_tag="pgaptmp_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HGU4561578.1"
                     /translation="AFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALP
                     ENYNRLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKG
                     YDQILNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYI
                     KRPSKDLANLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGN"
BASE COUNT          243 a          110 c          107 g          166 t
ORIGIN      
        1 gcgctttttt atcggctatt catcaccaga tcattgaaaa ccaactggat acagaaacaa
       61 taactattag ttatgaattt atcctctgtc aacatcttat cgcaaatacc gagttcagct
      121 atttggcact ccctgaaaat tataaccgtt tgtttttacc aaattcaaaa aaccaaacaa
      181 ataatcgttt caaaacgtta aacagcaaag ctattggcag gctacttgct gctggtggcg
      241 tatacaatgg gaacattgaa ggattcagag atactgcgga aaaactgggt ggagatgcaa
      301 taaaaggcta tgatcaaata ctaaatgaaa aaacagcggg catagcgata gcaacagcat
      361 ctattctttt aacaaagcgt tctaatgttg atacatatac agaaataaat agttacttag
      421 gcaaacttag aggtcaacaa aaacttcttg atggtataga cataatagaa ataatataca
      481 ttaagagacc ttcaaaagac ttagctaact tacgaaagga gtttaataaa actgtaagaa
      541 aaaattttct tatcaaactt gcaaaaacct ccgaagcatc tggaagattc aacgccgaag
      601 accttttaag aatgagaaag ggcaat
//
LOCUS       DAZKHC020000423          617 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZKHC020000423 DAZKHC020000000
VERSION     DAZKHC020000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.429"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>617)
                     /locus_tag="ACHOSM_RS26735"
                     /old_locus_tag="pgaptmp_005347"
     CDS             complement(<1..>617)
                     /locus_tag="ACHOSM_RS26735"
                     /old_locus_tag="pgaptmp_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HGU4561579.1"
                     /translation="DIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVRGTCQIERAASE
                     SPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEHNACVIRQQELD
                     FTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMK
                     TKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVP"
BASE COUNT          137 a          175 c          153 g          152 t
ORIGIN      
        1 tcaggaacgg gcgctgaata atgctctttc cgctctgcca tcacttcagc atccggacgt
       61 tcgccaattt tcgcctccca cgtctcaccg agcgtggtgt ttacgaaggt tttacgtttt
      121 cccgtatccc ctttcgtttt catccagtct ttgacaatct gcacccaggt ggtgaacggg
      181 ctgtacgctg tccagatgtg aaaggtcaca ctgtcaggtg gctcaatctc ttcaccggat
      241 gacgaaaacc agagaatgcc atcacgggtc cagatcccgg tcttttcgca gatataacgg
      301 gcatcagtga agtccagctc ctgctgacgg atgacgcagg cattatgctc gcagagataa
      361 aacacgctgg agggatcatc cggcgtccat ttgaggccaa acggcgtctc tttatcgcca
      421 aatttaaggt actgctcctc cccgcagtgc gggcaggcaa catgaaaacg cataaaatgc
      481 ggggattcac tggcagcacg ctcaatctga caggtgcctc tcacttttgg cgtggagcca
      541 cgtatggact ttggccagac tgagccttca atacgcttgt cacccaggaa cgtcggtgag
      601 ccttcctgtt caatatc
//
LOCUS       DAZKHC020000424          613 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZKHC020000424 DAZKHC020000000
VERSION     DAZKHC020000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.430"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..508)
                     /locus_tag="ACHOSM_RS26740"
                     /old_locus_tag="pgaptmp_005348"
     CDS             complement(<1..508)
                     /locus_tag="ACHOSM_RS26740"
                     /old_locus_tag="pgaptmp_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HGU4561580.1"
                     /translation="MPVDLTPYILPGVSFLSDIPQETLSEIRNQTIRGEAQIRLGELM
                     VSIRPMQVNGYFMGSLNQDGLSNDNIQIGLQYIEHIERTLNHGSLTSREVTVLREIEM
                     LENMDLLSNYQLEELLDKIEVCAFNVERAQLQVPESLRTCPVTLCEPEDGVFMRNSMN
                     SNVCMLYDK"
BASE COUNT          204 a          138 c           76 g          195 t
ORIGIN      
        1 ttttatcata caacatacaa acatttgaat tcattgaatt tctcataaat accccatctt
       61 ctggttcaca taatgtaaca gggcatgttc gtaagctctc tggcacttgc aattgtgcac
      121 gctccacatt aaatgcacat acttcaattt tatctaacaa ctcctctaac tggtagttag
      181 aaagcaaatc catattttcg agcatctcaa tttcacgcag tactgtaact tcacggcttg
      241 tcaaactacc atgattaagt gtacgttcaa tatgttctat atattgaagg ccaatctgga
      301 tattatcatt cgataaacca tcctggttaa gacttcccat aaaatatcca tttacctgca
      361 taggtcgtat tgacaccatc aactcaccca gtcttatttg agcttctcca cgaatagtct
      421 gattacgtat ctcagacaag gtttcttgag gaatatcaga caaaaaacta accccaggca
      481 aaatataagg cgttaaatct acaggcattc ctccccctcc attttaataa cttgattttg
      541 ctttagtaaa aaacaaaaga acataaaact atatttatat cattaattat cacttacaac
      601 aaaaagcttc tct
//
LOCUS       DAZKHC020000425          607 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZKHC020000425 DAZKHC020000000
VERSION     DAZKHC020000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.431"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            16..607
                     /locus_tag="ACHOSM_RS26745"
                     /old_locus_tag="pgaptmp_005349"
                     /pseudo
     CDS             16..607
                     /locus_tag="ACHOSM_RS26745"
                     /old_locus_tag="pgaptmp_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
BASE COUNT          198 a          112 c          118 g          179 t
ORIGIN      
        1 taagatcaaa tttatatggt ggcgaaaatt aatctcttat cacggcttac gccgctttta
       61 ttcgtgttcg ctcctttttt ggcacagtca aatatgactg tttatcctat ggctgtttcc
      121 attaatagtc agggtgaagg caacgttcgc gtaatatcta aaagtaatga agttcagtac
      181 atcaaggcga cagtattccg tatcgataat ccatcgacgc ctcaggaaaa tgaagttgag
      241 attaaatccg gtgatgcaaa tcatctggtt gttatgccac ctaaattcgc tttaccagcc
      301 ggtagtagca aaaccgtacg ttttgttgcg atggaaccag agcaaaaaga gaaaaattat
      361 cgcgttaaat ttgaagcggt tcccagtatt gatgacgttg ccacagataa aaaagatctc
      421 tctatgcagt taacagttaa cttaatttgg ggaatcgttg ttagtgttcc acctcagcaa
      481 cctatagcta agttataagt aaattctgcc caaaaattaa ttaatgcagg gaatcaacgc
      541 ttaaagcttt taacgatcgc ttattgtaaa aataatagca aagaaaactg taagatacag
      601 accgtaa
//
LOCUS       DAZKHC020000426          603 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZKHC020000426 DAZKHC020000000
VERSION     DAZKHC020000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.432"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(52..>603)
                     /locus_tag="ACHOSM_RS26750"
                     /old_locus_tag="pgaptmp_005350"
     CDS             complement(52..>603)
                     /locus_tag="ACHOSM_RS26750"
                     /old_locus_tag="pgaptmp_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4561581.1"
                     /translation="TWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAE
                     DLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFV
                     LATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCM
                     RGCELRRNVGNFGGFLSINKLSQ"
BASE COUNT          124 a          211 c          152 g          116 t
ORIGIN      
        1 cacaccgccg ggcatgcgcc agaatcgctg attctgtctg tgtcattgga tttactgcga
       61 aagtttgtta atggaaagga aaccgccaaa attgccgaca ttcctgcgca gttcacaccc
      121 gcgcatgcac ttgctgcatc tgtccttacg gatatccgtg gtggggttgt cgaactcatc
      181 cgccaccgca ggaccgttat acccgcattc atctccccgg taatcccaca tacaggtgtt
      241 cgccagcatg atgcgaccgg gaaacagcgc cccatccgtc tcggtcggtg tagccagcac
      301 aaacgaggcc gtcatggctg tcagctgcga catctgctcc accacccagc ggtcactcag
      361 ctcctgctcc gggtccgcct ccggattgcc cgcaacgaaa ttcaccgcat ccagaaaacg
      421 ggcatacacc cggcggcgga ccaccgtggc ccccaccaga ctctgcaggt cctccgccat
      481 cccggtgaca aggccaaaca gattggacac cgtcagcgac gggcgggcac tgctgccccg
      541 gccgttcatc tcaaagccgc tgccgtcaat cgggtatgcc tcatacttac gcccctgcca
      601 ggt
//
LOCUS       DAZKHC020000427          591 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZKHC020000427 DAZKHC020000000
VERSION     DAZKHC020000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.433"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            17..>591
                     /locus_tag="ACHOSM_RS26755"
                     /old_locus_tag="pgaptmp_005351"
     CDS             17..>591
                     /locus_tag="ACHOSM_RS26755"
                     /old_locus_tag="pgaptmp_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HGU4561582.1"
                     /translation="MFSIRSQLPISASVSIPAEQSQSISTIPGGRKIETVPEKEGLLV
                     FLGTKSVGEYALNILGQNVSRVTTGKKPYDILFLHEATKQDFDKKKTEFTFPGANRSY
                     LQSSNTDVAAAAAISIAATEMKTILPKDLTPEKYNKIYLPGDGSAGLPLLKCGDEFLS
                     PTDIVNRLVEHNLHEVEDIRLTSCHSANITK"
BASE COUNT          213 a          114 c          111 g          153 t
ORIGIN      
        1 ctaagaagga ggttgtatgt ttagtataag atctcagcta cctataagcg ccagcgtatc
       61 aattccggca gaacaatctc aatccatctc cacaattcct ggagggagaa aaatagagac
      121 agtgccagag aaagaaggat tgcttgtgtt tttaggaaca aaatccgtag gtgaatatgc
      181 tcttaatatt cttggtcaaa atgtttcaag agtcacaaca gggaaaaaac cttatgatat
      241 attattcctg cacgaagcta caaaacaaga ttttgataaa aagaaaacag agtttacctt
      301 tcctggtgca aacagaagtt atctacaatc aagtaacacc gatgttgctg ccgcagcagc
      361 tataagtatt gcggcaacag agatgaaaac tatcctacca aaggatttaa caccagaaaa
      421 atacaataaa atttatctgc caggggatgg ttctgctggc ctacctcttc ttaagtgtgg
      481 agatgaattt ttatcaccaa ctgatatcgt caatcgtctg gtagaacata atcttcatga
      541 agtagaagac atcagattaa catcctgtca ctcagccaac ataacaaaaa a
//
LOCUS       DAZKHC020000428          587 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZKHC020000428 DAZKHC020000000
VERSION     DAZKHC020000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.434"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>587
                     /locus_tag="ACHOSM_RS26760"
                     /old_locus_tag="pgaptmp_005352"
     CDS             <1..>587
                     /locus_tag="ACHOSM_RS26760"
                     /old_locus_tag="pgaptmp_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU4561583.1"
                     /translation="YKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISDSFGGPL
                     SLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAAGGGVAV
                     LAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAGVISDEAGGGIRQAAENL
                     ARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKV"
BASE COUNT          121 a          151 c          204 g          111 t
ORIGIN      
        1 gtataaggct gccatgcgca cactgcccgc acagttcacg gatattgtca ctcagcttgc
       61 cggtggtcag aatcccttcc ttatcatgct gcagcagggg gggcagatca gcgattcatt
      121 cggtggaccg ctcagcctgc ttaccctgct gaaggaggaa cttctcggga tcagggatgc
      181 ctctgaatca tcagaggagt cgctgtcaga tacggcaaat gcactggctg aaaatgcccg
      241 gaatgccggt gagctgggac gatttatgtc ggtggcccgt gtggcggcag gtggcggggt
      301 tgccgtactg gccgcgcttg ctgccgccgc ctggcaggca gagcaggctg accgggcctt
      361 attgcgttca ctgaccctga ccggaggggc tgctgccacc acaacggcag aattgtggaa
      421 aatggccggg gtgatcagcg atgaagccgg tggtggtatc agacaggcgg cagaaaatct
      481 ggcccgtctg gcagaaagcg ggaaatatac cgccgggcag ctacggatca tgggggaaac
      541 ctctcagaga tggctgcaga cggtggggga cgatgccggg aaggtgg
//
LOCUS       DAZKHC020000429          583 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZKHC020000429 DAZKHC020000000
VERSION     DAZKHC020000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.435"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>583)
                     /locus_tag="ACHOSM_RS26765"
                     /old_locus_tag="pgaptmp_005353"
     CDS             complement(<1..>583)
                     /locus_tag="ACHOSM_RS26765"
                     /old_locus_tag="pgaptmp_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4561584.1"
                     /translation="LWEIDLTVQGGERYFFCNELNEKGEAVTWQGRQYQAYPIDGSGF
                     EMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGATVVRRRVYARFLDAVNFVAGNPE
                     ADPEQELSDRWVVEQMSELTAMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDEC
                     GYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRG"
BASE COUNT          111 a          218 c          139 g          115 t
ORIGIN      
        1 tgccgcgcat ctcacacccg cgcatgcact tgctgcatct gtccttacgg atatccgtgg
       61 tggggttgtc gaactcatcc gccaccgcag gaccgttata cccgcattca tctccccggt
      121 aatcccacat acaggtgttc gccagcatga tgcgaccggg aaacagcgcc ccgtccgtct
      181 cggtcggtgt cgccagcaca aacgaggctg tcatggccgt cagctctgac atctgctcca
      241 ccacccagcg gtcgctcagc tcctgctccg ggtccgcttc cggattgccc gccacaaaat
      301 tcaccgcatc cagaaaacgg gcatacaccc ggcggcggac caccgtggcc cccaccaggc
      361 tctgcaggtc ctccgccatt ccggtgacca gaccgaacag attcgacacc gtcagcgacg
      421 ggcgggcact gctgcccttc ccgttcatct caaagccact gccgtcaatc gggtatgcct
      481 gatattgccg cccctgccag gtgaccgcct cccctttttc attcagctca ttgcagaaaa
      541 aataccgctc accaccctgt accgtcaggt cgatttccca gag
//
LOCUS       DAZKHC020000430          580 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZKHC020000430 DAZKHC020000000
VERSION     DAZKHC020000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.436"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..67
                     /locus_tag="ACHOSM_RS26770"
                     /old_locus_tag="pgaptmp_005354"
     CDS             <1..67
                     /locus_tag="ACHOSM_RS26770"
                     /old_locus_tag="pgaptmp_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4561585.1"
                     /translation="KSKTFKTENGARRWLERNSGE"
     gene            69..197
                     /gene="ydfB"
                     /locus_tag="ACHOSM_RS26775"
                     /old_locus_tag="pgaptmp_005355"
     CDS             69..197
                     /gene="ydfB"
                     /locus_tag="ACHOSM_RS26775"
                     /old_locus_tag="pgaptmp_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HGU4561586.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSNG"
     gene            227..445
                     /gene="ydfC"
                     /locus_tag="ACHOSM_RS26780"
                     /old_locus_tag="pgaptmp_005356"
     CDS             227..445
                     /gene="ydfC"
                     /locus_tag="ACHOSM_RS26780"
                     /old_locus_tag="pgaptmp_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HGU4561587.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
BASE COUNT          186 a          125 c          153 g          116 t
ORIGIN      
        1 caaaagcaaa acgtttaaaa ccgaaaatgg tgcccgtcgc tggctggaaa gaaactcagg
       61 tgagtgatat ggatttcgac acaatcatgg aaaaggctta cgaagaatac ttcgaaggcc
      121 ttgccgaagg cgaagaagct ctcagcttca gtgagtttaa acaggcgctt tccagttcgg
      181 caaaatctaa cggctgataa gcgaaacagc accgcgagga atcagtatgc agaaacgaga
      241 acccgtcatc atcgcgccag actataccga tgatgaactt tatgagtgga tgcgccagaa
      301 aattaatgca gcgcaggatc tgaaatgggc taatgaagcc agggctaagc aggctgaaaa
      361 tctgtccgct ctggagcagg atatcaccaa tctggaaaaa gcagcggcat taagcattgc
      421 cagaatgatt acatacccgc gttaatagct aaccaacgaa gctaaggttg gtaattaagg
      481 agttctccac gggtgaggtg gagtgcgtgc gccggacacg ggtgagcatc cggcactgac
      541 agtttactga aaggatattt ccctgaaaag tcagaccata
//
LOCUS       DAZKHC020000431          580 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZKHC020000431 DAZKHC020000000
VERSION     DAZKHC020000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.437"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>580)
                     /locus_tag="ACHOSM_RS26785"
                     /old_locus_tag="pgaptmp_005357"
     CDS             complement(<1..>580)
                     /locus_tag="ACHOSM_RS26785"
                     /old_locus_tag="pgaptmp_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4561588.1"
                     /translation="HLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQALPEELRL
                     SPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHREA
                     AGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYG
                     RDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWT"
BASE COUNT           99 a          206 c          178 g           97 t
ORIGIN      
        1 gtccagccat agcgcaccag cggcgcggca ggtaaattct ccgggtattc cgggtcgtgc
       61 gtcagccaca cagcagacag acggatgccg ttgtcccggc catattcggt gtaacccggc
      121 agggtatcag gaaaagcgga cggttccgtc ccgccggaaa tggcctgctg ccgggcttct
      181 tctgcccgct gcgcctgcgt ggtcagtacc agccggaagt gacgcccggc accatccgtc
      241 acgccggtga tttcgccgct gaattcaccg gcggcttcgc ggtggaacgt ctgtgtgcgc
      301 ccgaagcggt ccaccagccc ggtcagtacc cggtacggcg gcagcggcgc aggcagcacc
      361 tcatccgctt ccggcacccg ctcacaccag ccgagcagcc accacggccc ctgcggactg
      421 tttgtcgcca gataacgatg cggacttaag cggagttctt ccggcagcgc ctgccagagt
      481 gcggccagcc ggtgaccttc atccagtttc gccacgccgc cgcgcaccag ccacagtgac
      541 tcgctgcggc tgtaaccgtc ctcaccggga aacaggtgct
//
LOCUS       DAZKHC020000432          563 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZKHC020000432 DAZKHC020000000
VERSION     DAZKHC020000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.438"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..163
                     /locus_tag="ACHOSM_RS26790"
                     /old_locus_tag="pgaptmp_005358"
     CDS             <1..163
                     /locus_tag="ACHOSM_RS26790"
                     /old_locus_tag="pgaptmp_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretion protein EspJ"
                     /protein_id="HGU4561589.1"
                     /translation="ENSSWVSASVGSLLDKGAKVYPDISCSLRLGEPFIITLPETVRL
                     DVNIHPLKK"
     gene            complement(176..397)
                     /locus_tag="ACHOSM_RS26795"
                     /old_locus_tag="pgaptmp_005359"
     CDS             complement(176..397)
                     /locus_tag="ACHOSM_RS26795"
                     /old_locus_tag="pgaptmp_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561590.1"
                     /translation="MDIQCTLANTFPNEHPEAFILVTSAEKFIVDGNCIQLIGIGNEQ
                     GEFDFYRQIYPVTGGEHKVEALMWTHLPA"
BASE COUNT          174 a          133 c           99 g          157 t
ORIGIN      
        1 agaaaatagt agttgggtat cggcttctgt tggttctctt ctggacaaag gagccaaagt
       61 atatccagat atatcatgct ctctgagact gggagaacca ttcattatta cacttccaga
      121 aacagtcagg ctggatgtta atatccaccc actcaaaaaa tgatcctgaa cgtttttatg
      181 caggcaaatg cgtccacatc aaggcttcaa ccttgtgctc tccccctgtc acaggataaa
      241 tttgcctgta aaaatcaaac tctccctgct cattaccaat accaatcagt tgaatgcagt
      301 tgccatctac aataaatttt tccgctgacg taaccaaaat aaaggcttcc ggatgttcat
      361 taggaaaggt gttagcaaga gtacactgaa tatccatctc caacaaaatc tcctgattat
      421 tagcgtaaac tcatcgacga aatcgttact atcccctggg cacatatgta ccattgaata
      481 ctactgttct ccagattctg ataatccatg gcgaacaata agaacactac agccgctttg
      541 ctgattagtt agccataacg gtg
//
LOCUS       DAZKHC020000433          561 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZKHC020000433 DAZKHC020000000
VERSION     DAZKHC020000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.439"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>561
                     /locus_tag="ACHOSM_RS26800"
                     /old_locus_tag="pgaptmp_005360"
     CDS             <1..>561
                     /locus_tag="ACHOSM_RS26800"
                     /old_locus_tag="pgaptmp_005360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU4561591.1"
                     /translation="LPIWSEGLRINGLIKSKSVSFIDSNSIYFVFGGQIDYGRGIECI
                     LSAAKIAHKKNNKIKTIIIGRGRLVDQVIKDAESIENGIIYSDFIPRNDYLTLVSVCR
                     AGLIATVPNVSVPTYPSKCLDYMKLSIPIIASIEDTTDFGEIIINAGAGLCCNAGDSN
                     ALAAAMLSLAEDELLASQMGEKANLFF"
BASE COUNT          184 a           84 c          117 g          176 t
ORIGIN      
        1 ctgccaattt ggagtgaagg attaagaata aatggcttaa taaaaagtaa aagtgttagt
       61 tttattgatt caaattcaat atatttcgtt tttggagggc aaattgatta cgggcgtggg
      121 atagaatgca tattatcagc tgctaaaatt gctcataaaa aaaataataa aattaaaacc
      181 atcatcatag ggcgaggaag acttgttgat caggtaatta aagatgctga gagtatagaa
      241 aatggtatta tttactcaga ctttattcca cgaaatgatt atcttacact ggtttctgtt
      301 tgtcgcgcgg gtcttattgc tactgtacct aatgtttcag tcccaacgta cccatcaaaa
      361 tgcttggatt atatgaagct cagtatccca atcattgctt caattgagga cacaacagat
      421 ttcggtgaaa taataataaa tgcaggtgcg gggttgtgtt gtaatgcggg agattctaat
      481 gccctcgccg ccgcaatgct atcattggct gaagacgaat tattagctag tcaaatggga
      541 gaaaaagcaa atttattttt t
//
LOCUS       DAZKHC020000434          553 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZKHC020000434 DAZKHC020000000
VERSION     DAZKHC020000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.440"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(5..319)
                     /locus_tag="ACHOSM_RS26805"
                     /old_locus_tag="pgaptmp_005361"
     CDS             complement(5..319)
                     /locus_tag="ACHOSM_RS26805"
                     /old_locus_tag="pgaptmp_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4561592.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
BASE COUNT          160 a          146 c           80 g          167 t
ORIGIN      
        1 ttttttagca tttttttgac cgcgcgtttc cggacgtatt ctgttctcct gtccctttat
       61 atcgtcggaa tacccgccgc tcttcaaatc ccattcccaa ctcagaatgt agtctgttga
      121 ccgcttgttt tatttcggtc aggttcaccg gtgaaaccgg agtccggcgc gccttacgca
      181 aacactctgc tcgtttctgt gccgccactt ttcttttctg gtcatcactt agctgtacca
      241 tcacttttgc ccatcgttca gctgctctcc ggtacagtcc ttttttctcc agacattctg
      301 ccacgtgatc atgtagcata agtgacctcc gattatctac agactgccat cctgaattta
      361 ccttccctta atgaaataac aataaaaaac aaaccacgca aaaacaataa aacaacacac
      421 aaaaaaaaac taaataataa acaaaaataa tcaccttatt ttattatttt ttgagggagc
      481 aattactgaa caaaaaacgc tgactatata ctcaaaacca aacaactatt ctgccaatca
      541 ggtatcatgg caa
//
LOCUS       DAZKHC020000435          552 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZKHC020000435 DAZKHC020000000
VERSION     DAZKHC020000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.441"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>552
                     /locus_tag="ACHOSM_RS26810"
                     /old_locus_tag="pgaptmp_005362"
     CDS             <1..>552
                     /locus_tag="ACHOSM_RS26810"
                     /old_locus_tag="pgaptmp_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4561593.1"
                     /translation="ARNAGISASQAEENAANADTSAGDASESARQAAESAAAAKQSEE
                     ASSSSASAAAQKASESSQSAAEAELSRKTAESAAGNASRDATTAAEKARESAESAQSA
                     EQSRIAAEEAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERGDTGPAGATGERGPAG
                     DAGPAGPQGPKGDRGERGETGLTG"
BASE COUNT          150 a          144 c          203 g           55 t
ORIGIN      
        1 gcgaggaatg ccggcatatc agccagtcag gcagaagaga acgctgcaaa tgctgacact
       61 tcagcagggg atgcatcgga gtcagcccgg caggcggcag aaagtgcagc cgctgcaaag
      121 cagtcagagg aggcgtcctc gtcctcggcc tctgcggccg ctcaaaaagc cagtgagtca
      181 tcacaaagtg cagcagaagc tgaattgtca agaaagacgg cagaaagtgc agccggtaat
      241 gcatccaggg atgcaacgac cgcagcagaa aaagcccggg agtcagcaga aagcgcacag
      301 tcagcggaac aaagcaggat tgcggcggaa gaggccgtaa accgaatccc caccgtggtg
      361 ggacctcccg ggccaaaggg ggaaccgggg cccgcgggtc ctcaggggcc gaagggagat
      421 aaaggagagc gtggcgacac cggcccggca ggggcaaccg gtgaaagggg gccggcaggt
      481 gatgctggtc cggcaggacc acagggaccg aaaggcgaca ggggagagcg gggagagacc
      541 ggtctgacgg ga
//
LOCUS       DAZKHC020000436          545 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZKHC020000436 DAZKHC020000000
VERSION     DAZKHC020000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.442"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..292
                     /locus_tag="ACHOSM_RS26815"
                     /old_locus_tag="pgaptmp_005363"
     CDS             <1..292
                     /locus_tag="ACHOSM_RS26815"
                     /old_locus_tag="pgaptmp_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561594.1"
                     /translation="KVIQYQEECDDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRD
                     KNIASVFATGLNEWKQVKSAHVSKIRTLINEANLLIDFVLADTDKGKITKAD"
     gene            292..441
                     /locus_tag="ACHOSM_RS26820"
                     /old_locus_tag="pgaptmp_005364"
     CDS             292..441
                     /locus_tag="ACHOSM_RS26820"
                     /old_locus_tag="pgaptmp_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561595.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVMLVAIFFSTAWRVLTL
                     SGLVG"
     gene            449..>545
                     /locus_tag="ACHOSM_RS26825"
                     /old_locus_tag="pgaptmp_005365"
     CDS             449..>545
                     /locus_tag="ACHOSM_RS26825"
                     /old_locus_tag="pgaptmp_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4561596.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDNAIT"
BASE COUNT          142 a          103 c          150 g          150 t
ORIGIN      
        1 caaggtaatc cagtatcagg aagagtgtga cgatgttctc tatgaatact ggacgaaggg
       61 ttttgtcgtt aatccccgtc gaatgagtgt gatggaagaa ctcaatcagg cttgcgctga
      121 catgaaacgg gataaaaaca ttgccagtgt gtttgctacc gggctgaatg agtggaaaca
      181 ggttaaatcc gcgcatgtat caaaaatccg cacattgata aacgaagcga atctgctgat
      241 tgattttgtc ctggctgata cagacaaagg gaaaataaca aaggcggatt gatggagtgg
      301 tggctaatga tatcggataa actcataacg ctggcgaaga tcctctgtgt aatcgtcggc
      361 atttcatttt tagtcatgct ggttgccatt ttcttttcca ccgcctggcg agtcctgacg
      421 ttatcgggac tggtggggtg aaagagagat gaaccgtgtt ctgtgtgtgg tgattattgt
      481 cctgctggta gcctgtggtg cgcttagtct ggggctgaat cattaccgcg ataacgccat
      541 aacct
//
LOCUS       DAZKHC020000437          530 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZKHC020000437 DAZKHC020000000
VERSION     DAZKHC020000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.443"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..284)
                     /locus_tag="ACHOSM_RS26830"
                     /old_locus_tag="pgaptmp_005366"
     CDS             complement(<1..284)
                     /locus_tag="ACHOSM_RS26830"
                     /old_locus_tag="pgaptmp_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4561597.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDPEYYFVVVLAGQSNSMAYGEG"
     gene            261..401
                     /locus_tag="ACHOSM_RS26835"
                     /old_locus_tag="pgaptmp_005367"
                     /pseudo
     CDS             261..401
                     /locus_tag="ACHOSM_RS26835"
                     /old_locus_tag="pgaptmp_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            449..>530
                     /locus_tag="ACHOSM_RS26840"
                     /old_locus_tag="pgaptmp_005368"
     CDS             449..>530
                     /locus_tag="ACHOSM_RS26840"
                     /old_locus_tag="pgaptmp_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561598.1"
                     /translation="MAKHGAGCLPVNSVSAPESAITPYSFL"
BASE COUNT          137 a          147 c          124 g          122 t
ORIGIN      
        1 ccttcaccat atgccatgct gtttgactgc cccgccagaa ccacaacaaa gtaatactcc
       61 gggtctctgg tggcgctgat tactgtgcct tctccatccg acggcttcac caacacaggt
      121 gtggtgacat caccttccgc cgcaatggcc tgcatcagag tataaggcgt gatggccacc
      181 gggctgccaa atggctgcca cccctccttc agtttttgtg tcagcttttc cgcaagatct
      241 gacggcgacg ccgccctgac aacatcataa tgtttaatcg acattgaatt tctcccgtgt
      301 acaggaacag agttaaaaag ccggaaccgg aatcaaatca caggatggcc atgtgccagt
      361 ggctggtcgt aaaaaaaagg ccacgccatg cgcagccgga aataaaggga taatgatgat
      421 agtttgagaa aacagaaata acacttttgt ggcaaagcat ggtgccgggt gcctcccggt
      481 gaattcagta tcagcacctg aatccgcgat taccccatat tccttcttgc
//
LOCUS       DAZKHC020000438          515 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZKHC020000438 DAZKHC020000000
VERSION     DAZKHC020000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.444"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..365
                     /locus_tag="ACHOSM_RS26845"
                     /old_locus_tag="pgaptmp_005369"
     CDS             6..365
                     /locus_tag="ACHOSM_RS26845"
                     /old_locus_tag="pgaptmp_005369"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGU4561599.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
BASE COUNT          148 a           85 c          106 g          176 t
ORIGIN      
        1 tatttatggc taatacgacc actggtgtta ccagaattat taaagcagcg ggttattcat
       61 ggaaaggctt atgtgcggca tggcgctatg aagcagcctt tcgtcaggag tgcattctgg
      121 ttctgtcagc actgataata tccttttggc tggatgttga ctctgttact cgagtattac
      181 tcattggttc ggcaatgatg gtaattattg ttgaaataat taacagtgca attgaagcag
      241 ttgttgacag aattggttca gaacatcacg aattatcagg cagagcaaaa gatatgggtt
      301 cagccgcagt gttgattacg attttagttg ctatcattat atggggagtt ttattacttt
      361 cataacgccc aacatctaaa ttattcacag ttacctggtt aatatttact taaggataag
      421 aggtattcac ctcttatcct ctgaaaaatg tcagtatcta tctgcaggag gatataatat
      481 catcttcttt attataaact gaagtattta cattc
//
LOCUS       DAZKHC020000439          514 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZKHC020000439 DAZKHC020000000
VERSION     DAZKHC020000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.445"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>514)
                     /locus_tag="ACHOSM_RS26850"
                     /old_locus_tag="pgaptmp_005370"
     CDS             complement(<1..>514)
                     /locus_tag="ACHOSM_RS26850"
                     /old_locus_tag="pgaptmp_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4561600.1"
                     /translation="VKRQHRPALDLSRLPELLSRIGSYKGQPVTQLAVMLNLLVFIRS
                     SELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSKQVVAILAEL
                     QTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAMACSALIESG
                     LWSRDAVERQ"
BASE COUNT          128 a          128 c          128 g          130 t
ORIGIN      
        1 atctgacgtt caactgcatc gcgggaccac aaacctgatt ctattaacgc actacatgcc
       61 atcgcccgga atccatgacc acagacatcc tgggttgtgt cataccccat aaccctcagg
      121 gctttattta cggtgttttc actgattggt ttacgtgggt catgtgctcc cgtaaagatc
      181 agaccatttt caccagccca tgtctgaagt tctgccagta tggctacaac ctgtttgctg
      241 agtggcacaa gatgtggtgt tcgcatcttg gatccccggt gtgagaattt tacgcctggc
      301 agaggttcgc gttcggctgg aatagtccac atggcattgt caatatcaat ttcagaccag
      361 cgggcgtatc tgagttcact ggaacgaata aaaaccagta aattcagcat aacggcaagc
      421 tgggtgacag gttgcccctt gtaactgcca atacgcgaca aaagttcagg caggcgagaa
      481 agatcaagag cggggcgatg ctggcgtttt accg
//
LOCUS       DAZKHC020000440          514 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZKHC020000440 DAZKHC020000000
VERSION     DAZKHC020000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.446"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>514
                     /locus_tag="ACHOSM_RS26855"
                     /old_locus_tag="pgaptmp_005371"
     CDS             <1..>514
                     /locus_tag="ACHOSM_RS26855"
                     /old_locus_tag="pgaptmp_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4561601.1"
                     /translation="TGGRILSIDAASRTLTLDREVTLPETGAATVNLINGSGKPVSVA
                     ITAHPAPDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVCIRENTDGTFAITAVQHV
                     PEKEAIVDNGASFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQVKWDTPRVVKGVR
                     FSLRLTSGSG"
BASE COUNT           97 a          153 c          176 g           88 t
ORIGIN      
        1 tgaccggcgg acgtatcctg tccatcgatg ccgccagccg caccctgaca ctggaccgtg
       61 aggtgaccct gccggagaca ggtgccgcca cggtgaacct gattaacggc agcggtaagc
      121 cggtgagcgt ggccatcact gcacaccccg cgccggaccg gatacaggtc agtaccctgc
      181 cggatggtgt ggagacatac ggtgtgtggg ggctttccct gccgtcactg cgtcgtcgcc
      241 tgtttcgctg tgtctgcatc cgggaaaaca cggacggcac ttttgccatc acggcggtgc
      301 agcatgtgcc ggaaaaagaa gccatcgtgg ataacggggc cagctttgag ccgcagtcag
      361 gcaccctgaa cagcgtcatc cctccggcag tgcagcatct cacggtggag gtgagcgccg
      421 ctgacgggca gtatctggcg caggtgaaat gggacacgcc gcgggtggtg aagggggtgc
      481 gcttcagtct gcgcctgacc agcggaagcg gaga
//
LOCUS       DAZKHC020000441          507 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZKHC020000441 DAZKHC020000000
VERSION     DAZKHC020000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.447"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(308..>507)
                     /locus_tag="ACHOSM_RS26860"
                     /old_locus_tag="pgaptmp_005372"
     CDS             complement(308..>507)
                     /locus_tag="ACHOSM_RS26860"
                     /old_locus_tag="pgaptmp_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4561602.1"
                     /translation="TFMALARKHGCSDTCIGKRLHKAEGIVEGMLMMLGVRLEMDRYV
                     ERELPGGRTSVFYQRKNSLRS"
BASE COUNT          141 a          130 c           98 g          138 t
ORIGIN      
        1 gacattgctg ctttgacgct acggacataa aaaagccagc cactggggga ggctggcaaa
       61 ctcgtagagc aaaatgctgt tacgcaaact tcgttacagg gtcatcctgc aatacaaaaa
      121 atacacaata tttagaaaac taatagtgcc atgtgcaatt tttaagattt tgttattaat
      181 tgtggtcgca ccttcctttc tgtgtacttt ccgtatagct cacaggattc tgggtacaaa
      241 aaaacccgcg catcggcggg ttaagcagcg tggcaatgta accactctta tcatgatatg
      301 cagattttta cgatcgtaaa ctattttttc gctgataaaa tacagaggtt ctccctcccg
      361 gcaattcacg ctcaacatac cgatccatct caagcctcac tcccagcatc atcagcatgc
      421 cttcaacaat cccctccgct ttgtgaaggc gtttacctat acaggtgtca gagcacccat
      481 gtttccgtgc cagcgccatg aacgtct
//
LOCUS       DAZKHC020000442          507 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZKHC020000442 DAZKHC020000000
VERSION     DAZKHC020000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.448"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..240
                     /locus_tag="ACHOSM_RS26865"
                     /old_locus_tag="pgaptmp_005373"
     CDS             <1..240
                     /locus_tag="ACHOSM_RS26865"
                     /old_locus_tag="pgaptmp_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4561603.1"
                     /translation="AVKEVLRSEEVLSALKQKLRHNLEARLDAEVDAILDELLGVQAE
                     PPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            317..>507
                     /locus_tag="ACHOSM_RS26870"
                     /old_locus_tag="pgaptmp_005374"
     CDS             317..>507
                     /locus_tag="ACHOSM_RS26870"
                     /old_locus_tag="pgaptmp_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4561604.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIKED"
BASE COUNT          129 a          116 c          152 g          110 t
ORIGIN      
        1 gcagtgaagg aagtcctgcg ctctgaagaa gtcctgagcg cactgaaaca aaaacttcgc
       61 cataacctgg aagcgcgtct tgatgcagag gttgatgcca ttctggatga gctgcttggt
      121 gtacaggcag agccaccgac tgaagcggga gataccaccg cagagagcgg tgaagttcag
      181 cctgaatcac cggtcgccga tgcgactgaa cctcaacccg aatcggtcat gatgctgtaa
      241 cggggagtca gggccatcag taaacagctg ctggcctttt tcatgttgtg agcttccgga
      301 taacgggaga cggggtatgt accagatgga aaaaatcaca acaggtgtgt catacaccac
      361 gtcagcggtg gggacgggat actggttact gcagctgctg gacaaagtct ctccgtccca
      421 gtgggtggcg ataggtgtgc tggggagtct gctgtttggc ctgctgacgt atctgacaaa
      481 cctttatttc aagattaaag aagataa
//
LOCUS       DAZKHC020000443          499 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZKHC020000443 DAZKHC020000000
VERSION     DAZKHC020000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.449"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..291
                     /locus_tag="ACHOSM_RS26875"
                     /old_locus_tag="pgaptmp_005375"
     CDS             <1..291
                     /locus_tag="ACHOSM_RS26875"
                     /old_locus_tag="pgaptmp_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HGU4561605.1"
                     /translation="RDKALAWVEKNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYR
                     RINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
BASE COUNT          136 a          109 c          139 g          115 t
ORIGIN      
        1 cgtgataagg cgctggcatg ggtggagaaa aacatcaaag tgccgctgac cgaaccccag
       61 aaagcgggga tcgcgtcatt ctgtccgtac aacattggtc ccggtaagtg tttcccgtcg
      121 acgttttaca gacgaattaa tgcaggtgat cgcaggggag catgtgaggc gattcgctgg
      181 tggattaagg acggtggcag agactgccgt attcgctcaa acaactgcta cggtcaggtc
      241 tcacggcgtg accaggagag cgcgctggcg tgctggggaa ttgacagata agaagaatat
      301 tttgctgaaa aatgaggttt gcttacatgg acggataaca cgaaatcctg caaattggca
      361 aaatgtaagt gaataaagtc aaaacagttg tttaacactc aggcaccgta atgatgcctt
      421 tgtcatttct gcgcatctca cgcgcatctc acaacacaga acctttcagg atgacccttg
      481 aggataccgg tttggctgt
//
LOCUS       DAZKHC020000444          496 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZKHC020000444 DAZKHC020000000
VERSION     DAZKHC020000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.450"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>496
                     /locus_tag="ACHOSM_RS26880"
                     /old_locus_tag="pgaptmp_005376"
     CDS             <1..>496
                     /locus_tag="ACHOSM_RS26880"
                     /old_locus_tag="pgaptmp_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HGU4561606.1"
                     /translation="ENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPI
                     GLKGGWNLYTYPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINENKEYGGLI
                     CKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVTKENDV
                     YDSLN"
BASE COUNT          167 a           80 c          120 g          129 t
ORIGIN      
        1 gagaacccgc atcagctgca gcagcttatt cgtttgccgg ggcagcagta tgatgaggag
       61 tccggcctgt attataaccg ccaccgctat tatgacccgc tgcaggggcg gtatatcact
      121 caggatccga ttgggctgaa ggggggatgg aacctgtata catatccgct gagcccggtg
      181 aatagcatgg atccattagg attatatgaa tttaaatcaa aaaatataga tgatattgga
      241 atatttgcat tggcaatgtg taatggagaa tcaattaacg agaataaaga atatggtgga
      301 ctaatatgta agaagcaagg tgaatatttc cccatgaatc cgataagttc aaatgataat
      361 gatagtgtag acttgcgaaa tataaaatgc cctgaaggtt cagagagagt aggcgattat
      421 cacactcacg gtttttactc tgacgataaa ggaaataaag taacaaaaga aaatgatgtt
      481 tatgatagtc taaatt
//
LOCUS       DAZKHC020000445          491 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZKHC020000445 DAZKHC020000000
VERSION     DAZKHC020000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.451"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(80..391)
                     /locus_tag="ACHOSM_RS26885"
                     /old_locus_tag="pgaptmp_005377"
     CDS             complement(80..391)
                     /locus_tag="ACHOSM_RS26885"
                     /old_locus_tag="pgaptmp_005377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561607.1"
                     /translation="MEIVIYITISILILAIALIKQRMLANIETSNPYHAQYILRRRDD
                     YLVLFDRDALSCRFFYDAFPGMRLRHQVTGDTDDDRLAHSPADRMYTGKDHQKYTFCT
                     K"
BASE COUNT          136 a          102 c          120 g          133 t
ORIGIN      
        1 agtatttgat catgtataag caataaaaaa cagcttcagg taatgaggaa tatctcaatt
       61 tttaaacata aaatgtgaat tatttagtac aaaaagtgta cttctgatga tctttccctg
      121 tatacatccg gtctgcgggg ctgtgtgcca ggcggtcatc gtcggtatcg ccggtcacct
      181 ggtggcgcag acgcattccc ggaaaggcgt cataaaagaa gcggcagctc agggcatcac
      241 ggtcgaacag caccaggtaa tcatcgcggc ggcgcaggat gtactgggcg tgatacgggt
      301 tactggtttc gatattggct aacatccgct gttttattaa tgcgatagct aaaatcagga
      361 tggaaatagt gatgtatatc actatttcca tgatcttgcc ttgcgttcat ccaggcagga
      421 atatccctgt tagcagcctc tgtgcgatgt acgccagcgg tctgtaaaga aaccgattat
      481 cttataacaa a
//
LOCUS       DAZKHC020000446          486 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZKHC020000446 DAZKHC020000000
VERSION     DAZKHC020000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.452"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..132)
                     /locus_tag="ACHOSM_RS26890"
                     /old_locus_tag="pgaptmp_005378"
     CDS             complement(<1..132)
                     /locus_tag="ACHOSM_RS26890"
                     /old_locus_tag="pgaptmp_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4561608.1"
                     /translation="MSQAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     "
     gene            150..341
                     /gene="yfgG"
                     /locus_tag="ACHOSM_RS26895"
                     /old_locus_tag="pgaptmp_005379"
     CDS             150..341
                     /gene="yfgG"
                     /locus_tag="ACHOSM_RS26895"
                     /old_locus_tag="pgaptmp_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HGU4561609.1"
                     /translation="MSQVTSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          126 a          118 c           87 g          155 t
ORIGIN      
        1 atcattgtaa ataattaaat taacttccat aacattaaaa tatgtatcca ctgacgcttt
       61 tttacataac gaagaattga ccattttgtc ctgttgtgcc ttaatgtaag taccgtccac
      121 agcttgggac atacttcaag gaaccttttg tgagtcaggt aaccagtatg cgaaaacgac
      181 accgatttaa cagtcgcatg acccgtatcg tactgctcat cagctttatc ttcttctttg
      241 gccgttttat ctactcgtcc gtcggtgcct ggcagcacca tcagagcaaa aaagaagctc
      301 agcaatccac actctccgtc gaatcaccgg tacaacgtta gcggttacct tctccacttt
      361 cacagaacat aacggcactt cgctgtcgga tgcttttgct ctttgggatt atcaaagcgg
      421 cagatattct ttcatcttaa attttacgtc tttgtcctga ctgatgttta tcctgtttgg
      481 ctgcga
//
LOCUS       DAZKHC020000447          486 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZKHC020000447 DAZKHC020000000
VERSION     DAZKHC020000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.453"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(92..439)
                     /gene="tnpB"
                     /locus_tag="ACHOSM_RS26900"
                     /old_locus_tag="pgaptmp_005380"
     CDS             complement(92..439)
                     /gene="tnpB"
                     /locus_tag="ACHOSM_RS26900"
                     /old_locus_tag="pgaptmp_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4561610.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFLGRSGSQVKLLWSTGDGLCLLTKQLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSMTML"
BASE COUNT          112 a          131 c          132 g          111 t
ORIGIN      
        1 ctgtttcagc aggaagatgc cgttagaaga gatgtcgttc ataagcccgt attttaccgg
       61 gcttattctg tgacaaccag gataaagaga tttacagcat ggtcatggag gtcagcagcc
      121 gcttaggctg tcgccagtcg ataccttcca gcagcatcgc cagctgcgcc tgtgtaagga
      181 acactttgcc atcacgggct gacggccagg cgaagcgccc acgctccagc tgtttggtca
      241 ggaggcacag tccgtcaccg gtggaccaca gcagtttaac ctgactgccg ctgcggccca
      301 ggaaaatgaa aacatggccg gacatgggat cgtctttcag cgccgtctgt actttcgcag
      361 ccaggccgtt gaagccattt ctcatatcgg tgataccggc aaccagccaa attttggtcc
      421 cggaaggtaa agggatcatc gcttcagttc ctgtatcagc agagtcagga gcttttcgct
      481 gacatt
//
LOCUS       DAZKHC020000448          479 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZKHC020000448 DAZKHC020000000
VERSION     DAZKHC020000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.454"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            22..>479
                     /locus_tag="ACHOSM_RS26905"
                     /old_locus_tag="pgaptmp_005381"
     CDS             22..>479
                     /locus_tag="ACHOSM_RS26905"
                     /old_locus_tag="pgaptmp_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4561611.1"
                     /translation="MSEQKKTWAAEDQLRGSWMAGLKSGWSEWEESATDCMLQVKSAA
                     TQTFDGIAQNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVG
                     GGASASGGMAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAWG"
BASE COUNT          107 a          114 c          165 g           93 t
ORIGIN      
        1 ccgctggcgc tgaataacgt catgtcagag cagaaaaaga cctgggcggc tgaagaccag
       61 cttcgcggga gctggatggc aggcctgaag tccggctgga gtgagtggga agagagcgcc
      121 acggactgta tgttgcaggt aaaaagtgca gccacgcaga cctttgatgg tattgcacag
      181 aatatggcgg cgatgctgac cggcagtgaa cagaactggc gtagcttcac ccgttccgtg
      241 ctgtccatga tgacagaaat tctgcttaag caggcaatgg tggggattgt cgggagtatc
      301 ggcagcgcca ttggcggggc tgttggtggc ggcgcatccg cgtcaggcgg tatggcttac
      361 agccgtgtgt cgactttctc cggggattat ctccgcgtaa ctgacaacaa ggggaaaacg
      421 cacgacgtgc tgaccggaag tgatgacggt cgccacagca acacgtctct ggcgtgggg
//
LOCUS       DAZKHC020000449          479 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZKHC020000449 DAZKHC020000000
VERSION     DAZKHC020000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.455"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>479
                     /locus_tag="ACHOSM_RS26910"
                     /old_locus_tag="pgaptmp_005382"
     CDS             <1..>479
                     /locus_tag="ACHOSM_RS26910"
                     /old_locus_tag="pgaptmp_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4561612.1"
                     /translation="AGIMMTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDD
                     LDDLCQVQTHIRTITHRVTDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDM
                     LREAIGGLASRPEGFVVYTTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFE"
BASE COUNT          108 a          123 c          152 g           96 t
ORIGIN      
        1 gcggggatca tgatgactgc actgttactg aactggcggc aggcggcggg ttacacgatt
       61 ctggccccga cagtggaggt ggcagccaac gccttcaacc ctgccaggga tatggtacga
      121 cgggacgatg atctggatga cctctgtcag gtgcagacac atatccggac catcacccac
      181 agggtgacgg acaccaccct gaaggtggtg gcagccgatc cgaatacggt gtccggtatc
      241 aagtccgtgg ggacactgat tgatgaactg tggctgtttg gcaagcagta caaggcggag
      301 gacatgttac gtgaagccat tggcgggctg gcttcacgtc cggaagggtt tgtggtgtac
      361 acaaccaccc agtcaaatga gccgcctgcc ggggtgttca ggcagaaact gcagtacgcc
      421 cgggatgtcc gtgacggcaa aattcatgat ccgcactttc tgccggtgat atttgaaca
//
LOCUS       DAZKHC020000450          477 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZKHC020000450 DAZKHC020000000
VERSION     DAZKHC020000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.456"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>477)
                     /locus_tag="ACHOSM_RS26915"
                     /old_locus_tag="pgaptmp_005383"
     CDS             complement(<1..>477)
                     /locus_tag="ACHOSM_RS26915"
                     /old_locus_tag="pgaptmp_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4561613.1"
                     /translation="KETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQL
                     RAVAAGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNM
                     VVAAGLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRA"
BASE COUNT          102 a          150 c          118 g          107 t
ORIGIN      
        1 gcacgcaccc agtcagattc tgtcgctgca ccaccccgga tctgaattct ccaggcttca
       61 gcttccttaa ccgggtcgat ccacggcatc accggaccgg aatacgtcgc gttatatagc
      121 gttttcatct ccacatccgc cggaattttc agcagacctg ccgcaaccac catattcagc
      181 catgtccggt acaccgggcg ggttaccgcg ccaataaaac agtcctgcag gatcaggtaa
      241 ccatccgtgg actcgaccag ctcctgccgc tgggcgctgt aggtgccgtt atagttacgc
      301 gccgcactgg aaaaactcag acgactgcct gctgccactg cacgcaactg gccgttgcgg
      361 aaagtttcaa ggttgggatt gggacggtca gatttgacca tgccgatatc ctcgcccttg
      421 cgcaaatcgt cataaataat acccggggtg atatggactt cccgctcggt ctctttg
//
LOCUS       DAZKHC020000451          459 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZKHC020000451 DAZKHC020000000
VERSION     DAZKHC020000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.457"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..367
                     /locus_tag="ACHOSM_RS26920"
                     /old_locus_tag="pgaptmp_005384"
                     /pseudo
     CDS             1..367
                     /locus_tag="ACHOSM_RS26920"
                     /old_locus_tag="pgaptmp_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          125 a          112 c          103 g          119 t
ORIGIN      
        1 cagtgaaggt aggatcgcgc gtatatgtag tatgtttcaa atcaacagga tactacataa
       61 agggaggtcc agatagttac agctatctgg acctggaaca aattcgcatc aatgctttac
      121 ttcgagctcg tgtcgtatcc tatgatattc cccggtctcc tgtaaagcag agatgcataa
      181 aaaggagatc tttatcatgg ataaagagcc tgtttgccct gcctgtaaga ctcccttaca
      241 gctcatctat cgccgttctc gatctcaaaa tggcaagaca tacaaaccca aaaatgcgtc
      301 ctgtttcagg atgctgatat gtcctgcctg ttctccccgg agggaaagta cacaaaacgt
      361 gcattaacaa attcacccgg cagggaaacc tgccggtcgg tattgttaca tcatagtacc
      421 gtcagttccg ggctgaaccg gctctggttg ctctacggc
//
LOCUS       DAZKHC020000452          452 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZKHC020000452 DAZKHC020000000
VERSION     DAZKHC020000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.458"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>452
                     /locus_tag="ACHOSM_RS26925"
                     /old_locus_tag="pgaptmp_005385"
     CDS             <1..>452
                     /locus_tag="ACHOSM_RS26925"
                     /old_locus_tag="pgaptmp_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HGU4561614.1"
                     /translation="HLACFSGVLQADAYAGFNELYRNGGITEAACWAHARRKIHDVHV
                     RIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTL
                     SRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRK"
BASE COUNT          112 a          114 c          142 g           84 t
ORIGIN      
        1 catcttgcct gcttcagcgg tgtgctgcaa gcggatgcgt acgccgggtt caacgagctg
       61 tatcgcaatg gtgggataac ggaagctgcc tgctgggctc atgcccgccg aaagatccac
      121 gatgtgcacg tccgcatccc gtcagcactg acggaagaag ccctggagca gatcggtcag
      181 ttgtacgcca tagaggcgga tataagggga atgccggcag agcagcggct tgctgaacgt
      241 cagcgaaaaa cgaaaccgtt gttgaaatcc ctggaaagct ggttgcgtga aaagatgaag
      301 accctgtcgc gacactcaga gttggcgaag gcgttcgcgt acgcacttaa ccagtggccg
      361 gcactgacgt actatgcgaa cgatggctgg gtggaaatcg acaacaacat cgctgaaaat
      421 gccctgcggg cggtcagtct gggtcgtaaa aa
//
LOCUS       DAZKHC020000453          440 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZKHC020000453 DAZKHC020000000
VERSION     DAZKHC020000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.459"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>440)
                     /locus_tag="ACHOSM_RS26930"
                     /old_locus_tag="pgaptmp_005386"
     CDS             complement(<1..>440)
                     /locus_tag="ACHOSM_RS26930"
                     /old_locus_tag="pgaptmp_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4561615.1"
                     /translation="GSDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRW
                     HEDSVRNRWFSVMAGPSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVL
                     TGSDDGRHSNTSLAWGAGVQFNPTESVAIDIAYEGSGSGDWRTDG"
BASE COUNT           99 a          143 c          104 g           94 t
ORIGIN      
        1 ccgtcagtgc gccagtcgcc actgccggaa ccttcataag caatatcaat ggccacggat
       61 tcggtcgggt taaactgcac gccagccccc cacgccagag acgtgttgct gtggcgaccg
      121 tcatcacttc cggtcagcac atcatgcgtt ttccccttgt tgtcagttac gcggagataa
      181 tccccggaaa aagtcgacac acggctgtaa gccacgcccg ccatcgcata cgcactgaac
      241 cattcattca cgcgcacaga gggccccgcc atcacgctga accagcggtt acgaacggaa
      301 tcttcatgcc agcgggtatc gctgtaacgg gtcagctggc gattcttgtc tcctgcatag
      361 ctgaaagacg tcaccatccc cagtgtgtcc gtaaactcat aacggtattt cacgttaatc
      421 ccgttcagat catcgctgcc
//
LOCUS       DAZKHC020000454          438 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZKHC020000454 DAZKHC020000000
VERSION     DAZKHC020000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.460"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          139 a           86 c           81 g          132 t
ORIGIN      
        1 tcaactaatt ggaggcatta ccatgcatta gtgcaagcgc cttgtggatt cttttgctaa
       61 tgacggaaac atattgtaca atattgcatt tatccctcaa taaataaatg agttatcaga
      121 ttgcaaaaat ctgttatcaa actaattcaa ataaaaagag attgatacga ccaacgtaca
      181 caaaaatttt cgatattcga actaaataac agctcctaat accgctacac tttagctatc
      241 cagtctgcgc ctcagaaatg gacgatgccg ttgaaggact ggtgtatggc aatgagccgc
      301 tttattatcg aattcggtga ccgcttggac ggtcacttcg gagaaaaggc atttacacag
      361 catcctaaac aggttcagca taggtgatat accgcttttt cattttcttc tccattattt
      421 ataagggagg tgtattat
//
LOCUS       DAZKHC020000455          434 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZKHC020000455 DAZKHC020000000
VERSION     DAZKHC020000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.461"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..181)
                     /locus_tag="ACHOSM_RS26935"
                     /old_locus_tag="pgaptmp_005387"
     CDS             complement(<1..181)
                     /locus_tag="ACHOSM_RS26935"
                     /old_locus_tag="pgaptmp_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HGU4561616.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSY"
     gene            complement(135..335)
                     /locus_tag="ACHOSM_RS26940"
                     /old_locus_tag="pgaptmp_005388"
     CDS             complement(135..335)
                     /locus_tag="ACHOSM_RS26940"
                     /old_locus_tag="pgaptmp_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4561617.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPYHQHA"
BASE COUNT          103 a           91 c          107 g          133 t
ORIGIN      
        1 ggtaggagtt atttacgaaa cggtggctgt aatgtttatt gacgatgacc agtctggcca
       61 gccagcaact tattgtctca acccgcaact cacgggagcc ataaccaacg atattgtcgt
      121 tatagcgaat aagttcaggc gtgctgatga tacgggatgt gacttgcttt ctgttcccca
      181 cgacaggatt tccttgtgta tgtggggtgc tctgtggcgc tcggagatgt gatttgattg
      241 agggttttac agcgcggaca ttttacttcc agataactga aactggcccg tgccagtagc
      301 ttattgcagt gtcggcaccg tatgtttcga tacatggccg cataacctcc tgctttgttg
      361 ttgatgcctt tttcatgcaa tcgatcgaca aaaacgatcg attcgatttg tttaattgat
      421 acaacaaata gatt
//
LOCUS       DAZKHC020000456          434 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZKHC020000456 DAZKHC020000000
VERSION     DAZKHC020000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.462"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>434)
                     /locus_tag="ACHOSM_RS26945"
                     /old_locus_tag="pgaptmp_005389"
     CDS             complement(<1..>434)
                     /locus_tag="ACHOSM_RS26945"
                     /old_locus_tag="pgaptmp_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system domain-containing
                     protein"
                     /protein_id="HGU4561618.1"
                     /translation="DLTGVNQVSFRHLRESSSTRNQLNKILRQEIQDKISGVELSDIN
                     KEGFLYQSIGKIRLLALSSALFEIQCPDYIFSRLYRETLIREIGYQNVKQLSFYWQGG
                     QCKPEYGEERFCSELIKYGAGNLEWLFSDNPLWTIVKYLLPK"
BASE COUNT          138 a           88 c           60 g          148 t
ORIGIN      
        1 ttttcggtaa aagatatttg actatagtcc ataagggatt atcagaaaat agccattcaa
       61 gatttcctgc accgtatttt atcagttcag aacaaaaccg ttcttcgcca tattctggtt
      121 tacattgccc tccctgccaa tagaatgata actgcttcac attttgatat cctatctctc
      181 tgatcagagt ttctcgatat aatcgtgaaa aaatatagtc agggcattga atttcaaata
      241 atgcactact tagagcaagc aatcttattt tcccaattga ctgatataaa aaaccttcct
      301 tatttatatc agataattcc actccagaaa ttttatcttg tatttcctga cgcaatatct
      361 tgttgagttg attacgagta gaactacttt ctcttaaatg ccgaaaacta acctgattga
      421 caccggttaa atca
//
LOCUS       DAZKHC020000457          431 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZKHC020000457 DAZKHC020000000
VERSION     DAZKHC020000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.463"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..359)
                     /locus_tag="ACHOSM_RS26950"
                     /old_locus_tag="pgaptmp_005390"
     CDS             complement(<1..359)
                     /locus_tag="ACHOSM_RS26950"
                     /old_locus_tag="pgaptmp_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HGU4561619.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRL"
BASE COUNT          119 a          112 c           91 g          109 t
ORIGIN      
        1 agccggtcag gccaggtttc cagtacctga actatcgctt tcaccacgac agaaaaggac
       61 cgcctgacat cactgactgg cacaagctga ccagtttcct gctcaaattt cagtcggtca
      121 cgttctgact ggtaccatgc tttacgcgca tgaggatcca tttcctcgtt atctacaggc
      181 agaggagctt tcatcagctc ggcaaggata tctgtcagtc ggtacagttt gagattgctc
      241 tcatgaccac cggctgggct tatgtttttt acccgagccg caacagtctg tcgatgagca
      301 ccggataatg cggccagttg ggaaatattc agatgcagat tttttaattc acgatccata
      361 actccccctg aaaattatgt aaacacacac cagtgatgaa caaaaaacaa ccagattcga
      421 cactaaaaat t
//
LOCUS       DAZKHC020000458          428 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZKHC020000458 DAZKHC020000000
VERSION     DAZKHC020000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.464"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>428)
                     /locus_tag="ACHOSM_RS26955"
                     /old_locus_tag="pgaptmp_005391"
     CDS             complement(<1..>428)
                     /locus_tag="ACHOSM_RS26955"
                     /old_locus_tag="pgaptmp_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4561620.1"
                     /translation="GRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVTGRDRETREWL
                     GWGHAWVHETAVVRRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLD
                     HIGIDPSGVGQILDSLAEAGIPDESVVGISQGWKLGGAIKT"
BASE COUNT           87 a          171 c           89 g           81 t
ORIGIN      
        1 gttttgatgg ccccgcccag cttccagccc tggcttatcc ccaccacact ctcatcgggg
       61 atcccggctt ccgccagtga atccagaatc tgccccacac ctgacgggtc aataccgata
      121 tgatccagta actcagcctc atgaatacga cgcacatact ccgccacttc cgccgtgtca
      181 tccccgaccc gacggacaat cgtcatgtct ccacaggcca caaaatcctg aaaccgggat
      241 gcctcactct tccgtctgac caccgcggtt tcatgcaccc aggcatggcc ccagcccagc
      301 cattcgcggg tttccctgtc acggccagtc acgtacattc ccagcagatc atccaggccc
      361 ccgccgtcaa tccccaccgt caccacatca gcgcgctgca ggatatcgtc caggctgacg
      421 cgcctgcc
//
LOCUS       DAZKHC020000459          420 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZKHC020000459 DAZKHC020000000
VERSION     DAZKHC020000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.465"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>420
                     /locus_tag="ACHOSM_RS26960"
                     /old_locus_tag="pgaptmp_005392"
     CDS             <1..>420
                     /locus_tag="ACHOSM_RS26960"
                     /old_locus_tag="pgaptmp_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4561621.1"
                     /translation="SGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCTNFV
                     FSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQELCS
                     VDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGM"
BASE COUNT          111 a           94 c          127 g           88 t
ORIGIN      
        1 tcgggcatcc agcctctgca cagcaaatgc tcgtttgcga attatcaggt gcagaacgac
       61 gggcaaaaat acgcgctgag ccaggccaaa tccatagctg acgaactgat gaccgggtgc
      121 acgaattttg tgttcagcgg taaaaccggc accgggaaaa atcaccttgc agcggcgatg
      181 ggtaaccggc tgatggcgaa ggggcgcagc gtgattatcg tcaccgtgtc tgatgtcatg
      241 agcgtgttgc atgacagcta cgacaacggc aaatccgggg aaaaattttt acaggagctt
      301 tgcagtgttg atttgctggt cctggatgaa ataggcgttc agcgggagac gaaaaacgag
      361 caggtggtat tacaccagat aattgatcgc cggacagcat cactgtgcag tgtcgggatg
//
LOCUS       DAZKHC020000460          419 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZKHC020000460 DAZKHC020000000
VERSION     DAZKHC020000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.466"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>419
                     /locus_tag="ACHOSM_RS26965"
                     /old_locus_tag="pgaptmp_005393"
     CDS             <1..>419
                     /locus_tag="ACHOSM_RS26965"
                     /old_locus_tag="pgaptmp_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HGU4561622.1"
                     /translation="DRLLGREHRMERRAVALERQLNGGVDFLSSVNNYFQSVMAEHRE
                     NKTSNKILMEKINSCLFRPDSNHFSCPESFLTCPITLDTPETGVFMRNSRSAEICSLY
                     DKDALVQLVETGGAHPLSREPITELMIMRKDECHFDT"
BASE COUNT          137 a           64 c          100 g          118 t
ORIGIN      
        1 tggataggct attggggcgt gagcatcgaa tggagagaag ggctgtggct ctggaaaggc
       61 aattaaatgg aggtgtcgat tttttaagta gtgttaataa ctattttcag agtgtcatgg
      121 cagaacacag agaaaataaa acaagtaata aaatattaat ggaaaaaata aattcttgtt
      181 tatttagacc ggattctaat cacttttctt gcccggagtc atttttgaca tgcccgataa
      241 cgctggacac acctgagact ggagtgttca tgagaaactc acgaagtgct gagatatgct
      301 ctctatatga taaggacgcg ttagtgcaac ttgttgaaac tggtggagct catcctctga
      361 gtcgagaacc tataacagaa ttaatgatta tgagaaaaga cgaatgtcac tttgataca
//
LOCUS       DAZKHC020000461          419 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZKHC020000461 DAZKHC020000000
VERSION     DAZKHC020000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.467"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..199)
                     /locus_tag="ACHOSM_RS26970"
                     /old_locus_tag="pgaptmp_005394"
     CDS             complement(<1..199)
                     /locus_tag="ACHOSM_RS26970"
                     /old_locus_tag="pgaptmp_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4561623.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLT"
     gene            256..419
                     /locus_tag="ACHOSM_RS26975"
                     /old_locus_tag="pgaptmp_005395"
                     /pseudo
     CDS             256..419
                     /locus_tag="ACHOSM_RS26975"
                     /old_locus_tag="pgaptmp_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
BASE COUNT          108 a          108 c          101 g          102 t
ORIGIN      
        1 tggtgagccc accatcaccg cccccggtat cccgctcatg ctggcgaacc cagacacgca
       61 gagtctccgg cgtacagcca atctttgggg caatggaaca aattgccgcc cactgtgagt
      121 catattcatc ctgactttcc agaaccatac gaatcgcccg ctgacggact tcgggggaaa
      181 aacgagtatt tttagtcatc ctgtttacct ctttctcagg gagtttagtc tccaggattt
      241 ccggggcggt tcagtgagag aatgctaagt atgctttcgg actcaggtga agcacaatcg
      301 caagagagta ttcaggacaa aatatctcaa tgtaagtttc ctgttagttc aggaaatttc
      361 cagtgcccgc cagagtctat tcagtgtcca attacactag agagacccga agaaggagt
//
LOCUS       DAZKHC020000462          414 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZKHC020000462 DAZKHC020000000
VERSION     DAZKHC020000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.468"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>414)
                     /gene="espL2"
                     /locus_tag="ACHOSM_RS26980"
                     /old_locus_tag="pgaptmp_005396"
     CDS             complement(<1..>414)
                     /gene="espL2"
                     /locus_tag="ACHOSM_RS26980"
                     /old_locus_tag="pgaptmp_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HGU4561624.1"
                     /translation="SPGHAMALGIKIKNDKENKFVINFYDPNQTATHKRVFFCTNNIC
                     DIINLTAYDFLSEQCLKCYGLKEDTLSLFVDKTKSNDNNNVFIKKLPDNILQGVVINF
                     AMGAGLREIIKKVYNDTRFTDLTKSQMKILCESKNV"
BASE COUNT          125 a           71 c           60 g          158 t
ORIGIN      
        1 acattttttg attcacacaa gattttcatc tgagattttg taagatctgt aaatcgagta
       61 tcgttatata ccttttttat tatctctctt agtcctgctc ccatagcaaa attaataaca
      121 acaccttgga gaatattatc aggtaatttt tttataaaca cattattgtt gtcattcgat
      181 tttgttttat ccacaaaaag tgacagtgtg tcttctttta gcccataaca tttcagacat
      241 tgctcactaa gaaaatcata tgctgtcagg tttattatat cacaaatgtt attagtgcaa
      301 aaaaatactc gtttatgcgt cgcggtctga ttgggatcat agaaattaat aacaaactta
      361 ttttccttgt cgttttttat tttaatgccc agagccattg catgtccggg agaa
//
LOCUS       DAZKHC020000463          414 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZKHC020000463 DAZKHC020000000
VERSION     DAZKHC020000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.469"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>414
                     /locus_tag="ACHOSM_RS26985"
                     /old_locus_tag="pgaptmp_005397"
     CDS             <1..>414
                     /locus_tag="ACHOSM_RS26985"
                     /old_locus_tag="pgaptmp_005397"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGU4561625.1"
                     /translation="NNSEDKLIVFHLMGSHGPTYYLRYPESHKYFKPTCDRSDIENCT
                     HEQLINTYDNTIRYTDYIISKLIDKLIEYKDEYDTVLLYVSDHGESLGENGLYLHGTP
                     YNVAPAEQTHVPLITWMSPGFVSSKKIDLNCLSEHA"
BASE COUNT          135 a           71 c           71 g          137 t
ORIGIN      
        1 taataactca gaggataaac ttatcgtatt tcatttgatg ggtagtcatg gtcctaccta
       61 ctatctccgt tatcctgagt cacataaata ttttaaacca acatgtgatc gtagtgatat
      121 cgaaaattgt acgcatgagc aattgattaa tacctatgat aatacaattc gctatactga
      181 ctatataata tcaaaattaa ttgacaagct tatcgaatat aaagatgagt atgatactgt
      241 tttattatat gtctctgacc atggagaatc tctgggagaa aatggattat acttacatgg
      301 aacaccatat aatgttgcac cagctgagca aacgcatgtg cctcttatta cctggatgtc
      361 tccggggttc gtttcctcta aaaaaattga tttgaattgt ttgtcagaac atgc
//
LOCUS       DAZKHC020000464          414 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZKHC020000464 DAZKHC020000000
VERSION     DAZKHC020000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.470"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..410
                     /locus_tag="ACHOSM_RS26990"
                     /old_locus_tag="pgaptmp_005398"
     CDS             <1..410
                     /locus_tag="ACHOSM_RS26990"
                     /old_locus_tag="pgaptmp_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HGU4561626.1"
                     /translation="RPVQILYTYSDAGNSVFLVVDHLPWTDSDKINWYLKHQNEIKNQ
                     HPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDLYCFPTIKSNDNCIVKNYLMVINEY
                     PYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
BASE COUNT          142 a           73 c           73 g          126 t
ORIGIN      
        1 tgcgtcctgt acagattttg tatacatact ctgatgctgg caattcagtc tttctggtcg
       61 ttgatcatct gccatggact gacagcgata aaataaattg gtatctgaag catcagaatg
      121 aaattaaaaa tcaacatccc ctaccagaag gtagttggca tacctggtat gtcattgata
      181 tcggtaatgg cttcactgac tataaaaaat atatcgaagg gccgtatgaa gatctctact
      241 gtttcccgac aattaagagc aatgataatt gcatcgtgaa aaattactta atggttatta
      301 atgaataccc ttatcgtaac actcatattg gtattaacga ttttactgag tatcaattaa
      361 cacaagaaaa taaaatagag cgagttttca atccgcatga ctttaaataa gaca
//
LOCUS       DAZKHC020000465          413 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZKHC020000465 DAZKHC020000000
VERSION     DAZKHC020000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.471"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>413)
                     /locus_tag="ACHOSM_RS26995"
                     /old_locus_tag="pgaptmp_005399"
     CDS             complement(<1..>413)
                     /locus_tag="ACHOSM_RS26995"
                     /old_locus_tag="pgaptmp_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4561627.1"
                     /translation="TPEGERYIPCVNISAEPEAYFRIAPEDWLRAEMQGEIVALVHSH
                     PGGLPWLSEADRRLQIKSALPWWLVCRGDIHKFRCVPHLTGRRFEHGVTDCYTLFRDA
                     YHLAGTEMPDFHREDDWWRNGQNLYLDNMAATGFY"
BASE COUNT           87 a          141 c          104 g           81 t
ORIGIN      
        1 gtaaaaacca gtcgccgcca tattgtccag gtacaggttc tgaccgttgc gccaccagtc
       61 atcctcgcga tgaaaatccg gcatttcagt ccccgccaga tggtaggcat cccggaacag
      121 cgtgtaacag tccgtcaccc cgtgctcaaa gcgccgtcct gtcagatgtg gcacacagcg
      181 gaatttgtga atgtcacccc ggcagaccag ccaccagggc agtgcgcttt ttatctgcag
      241 ccgccggtca gcctcgctca gccagggcag tccaccggga tgactgtgga ccagtgccac
      301 aatctccccc tgcatctctg cccgcagcca gtcttccggt gcgatacgaa aatacgcctc
      361 cggctctgca gagatattca cacaagggat ataccgttcc ccctccggcg tgc
//
LOCUS       DAZKHC020000466          407 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZKHC020000466 DAZKHC020000000
VERSION     DAZKHC020000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.472"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>407)
                     /locus_tag="ACHOSM_RS27000"
                     /old_locus_tag="pgaptmp_005400"
     CDS             complement(<1..>407)
                     /locus_tag="ACHOSM_RS27000"
                     /old_locus_tag="pgaptmp_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4561628.1"
                     /translation="DIKVNLSHILIDSLDDAKVNLAPVIVSILETFLKSPYINDVRIL
                     DWCFNKGMQYFADSAKIKHACSVINHIDFSRDQSKDFSCDQSKIKIAETLFFNLDKEP
                     YKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLI"
BASE COUNT          136 a           67 c           65 g          139 t
ORIGIN      
        1 gtattaagcg tgatttttct tgattgctta agctaaaatc attgacatat gcaaccaact
       61 tatcccaaat caattcctgt aatttacggc tatttttata gggttcttta tcgagattga
      121 aaaataaggt ctcagctatt ttaattttag attgatcgca gctaaaatct ttagattgat
      181 cgcggctaaa atcaatatga tttatcacag agcatgcatg ctttatcttt gctgaatcag
      241 caaaatattg catgccttta ttaaaacacc aatccagtat tcttacatca ttaatatatg
      301 gggattttaa aaaagtctcc agaatcgaaa cgatgaccgg tgcaaggtta acttttgcat
      361 catctaagga atcaataaga atatgagaca ggttaacttt gatgtca
//
LOCUS       DAZKHC020000467          406 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZKHC020000467 DAZKHC020000000
VERSION     DAZKHC020000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.473"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>406)
                     /locus_tag="ACHOSM_RS27005"
                     /old_locus_tag="pgaptmp_005401"
     CDS             complement(<1..>406)
                     /locus_tag="ACHOSM_RS27005"
                     /old_locus_tag="pgaptmp_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HGU4561629.1"
                     /translation="EARILNDIWLITALGLVATAGLAWGAKYLEITATKYDSPPMYVA
                     IGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMF
                     LISMLAGLLFNVDLGSHRFIIMMTLTGLALVI"
BASE COUNT          136 a           95 c           77 g           98 t
ORIGIN      
        1 atgattacca gggccaaccc tgtcaacgtc atcataatga taaaacggtg agaaccaaga
       61 tcaacattaa ataataaacc ggccagcata ctgattaaaa acatcgcgcc cgcagtgctg
      121 aacacgatga taatggcata tacaggaact aaacttgcaa cgacaatcgc caccaaagag
      181 agtaaaaaca gatatattac gcccgcgatg gcggcattta tcttgttgat atccttactt
      241 aagccataca tacaaagtaa taataaccct atggcgacat acattggtgg tgaatcatat
      301 ttggttgccg taatttcgag atacttagct ccccaggcta gtcctgcggt tgccaccaaa
      361 cctaaagcgg taatgagcca tatatcgttt aatatcctgg cctcgt
//
LOCUS       DAZKHC020000468          405 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZKHC020000468 DAZKHC020000000
VERSION     DAZKHC020000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.474"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          146 a           75 c           61 g          123 t
ORIGIN      
        1 ccggaaagag tgagtactgg atactcaccc tgaatctatt ttaaatgaca tcaggaatac
       61 ctgtccatac ttaagatttt tcctatggaa aacaaacatt aatatatctt atatatttcg
      121 catggtgatg atttatacac ctgcactgaa tttttctgta tttccagaac tgaatcgata
      181 aacagaattg gtaataatga aagttaatca cataaaaaag aggttaccgc cttataatga
      241 taagagttca ttatccattc catccagaca aaacctgcct gtgtataaaa aataagaacc
      301 atgtcacaaa aacattacat tccggcaagt tatgacagaa ttactgaaaa ccgttatctg
      361 tagttattgt agtttctgat atatgctcat ccacccggaa atacg
//
LOCUS       DAZKHC020000469          398 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZKHC020000469 DAZKHC020000000
VERSION     DAZKHC020000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.475"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            13..>398
                     /gene="gpU"
                     /locus_tag="ACHOSM_RS27010"
                     /old_locus_tag="pgaptmp_005402"
     CDS             13..>398
                     /gene="gpU"
                     /locus_tag="ACHOSM_RS27010"
                     /old_locus_tag="pgaptmp_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HGU4561630.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKIT"
BASE COUNT           93 a           76 c          125 g          104 t
ORIGIN      
        1 ctgggagggt gtatgaaaca ccgtgaaata cgggcggcag ttctgtctgc cctgaaagaa
       61 aatatttctg agagggtgag ctggtttgac ggtcgcccgg tttttattga tgaacaggaa
      121 ctgcctgctg ttgctgttta cctgacagat gcgtctgctg ctgacgagtt cgttgatgag
      181 ggaacctggg aggcgacact gcatattgag gtttttctca gggcaaaaga accggactcg
      241 gcactggata tgtggatgga agaaaaaatt cttcctgcgc tggaggcagt tcccgggctc
      301 agtgcattac tgctgaagat gaatcttcag gggtatgact accgccggga tgatgagttt
      361 atgatgtggg gatcggcaga tctcctgtgg aaaattac
//
LOCUS       DAZKHC020000470          391 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZKHC020000470 DAZKHC020000000
VERSION     DAZKHC020000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.476"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(12..>391)
                     /locus_tag="ACHOSM_RS27015"
                     /old_locus_tag="pgaptmp_005403"
     CDS             complement(12..>391)
                     /locus_tag="ACHOSM_RS27015"
                     /old_locus_tag="pgaptmp_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4561631.1"
                     /translation="LSLFESDERILLFLSGCLSSISAKVRAIIQTDISASWTLGAIAL
                     RLHMSESLLKIKLKNEGHMFSRLLLEERMRVAVNMLCSRHGYGQAVAEKCGYSSWSYF
                     ISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          122 a           99 c           67 g          103 t
ORIGIN      
        1 tcagatgaaa atcaataatc aagcccttgc ctggatacat atctgtctgg cgggaagcca
       61 taatagcggt gaaatacaga aataaagtag gaccagcttg aataaccgca tttttctgct
      121 acagcctgtc catatccatg ccgggaacat aacatattga cagcaacacg catccgctct
      181 tccagcaaca agcgactgaa catgtgccct tcatttttca gttttatctt taacaaactc
      241 tcactcatat gcaggcgtaa cgcaatcgca ccaagcgtcc agcttgctga tatatctgtc
      301 tgaattatcg ccctgacttt ggcacttata ctggataaac atccacttaa aaataataat
      361 atccgttcat ctgattcaaa cagcgacagg c
//
LOCUS       DAZKHC020000471          389 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZKHC020000471 DAZKHC020000000
VERSION     DAZKHC020000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.477"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>389
                     /locus_tag="ACHOSM_RS27020"
                     /old_locus_tag="pgaptmp_005404"
     CDS             <1..>389
                     /locus_tag="ACHOSM_RS27020"
                     /old_locus_tag="pgaptmp_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4561632.1"
                     /translation="SKVEIEITLSTITTMGTRQGHTAIIFQQPDGSTNRYEGKSFERK
                     DESSLHLITNKILACYQREANKEIARLLNIPQELNNSHDLNNSQVSCKGSVDSTITDL
                     LEKPLNNALLAIRKEHLLLMPYVCNES"
BASE COUNT          138 a           64 c           72 g          115 t
ORIGIN      
        1 tctaaggtag aaatagaaat tacactttcc actatcacta ctatggggac tcgtcaagga
       61 catacggcta ttatatttca acaacctgat ggttcgacta atcgttatga aggaaagtcc
      121 tttgaaagaa aagatgagag ttcattacac ctgattacta acaagattct ggcgtgttac
      181 caacgcgaag ctaacaagga aatagctcgt ctattaaata ttccgcagga gttaaataat
      241 tctcatgact taaataactc gcaggtgagt tgtaaaggtt cagttgattc tacgattacg
      301 gatttattag aaaaaccatt gaataatgca ttattagcaa taaggaaaga acatctgcta
      361 ttaatgcctt atgtatgtaa tgaatcgat
//
LOCUS       DAZKHC020000472          384 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZKHC020000472 DAZKHC020000000
VERSION     DAZKHC020000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.478"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..290
                     /locus_tag="ACHOSM_RS27025"
                     /old_locus_tag="pgaptmp_005405"
     CDS             <1..290
                     /locus_tag="ACHOSM_RS27025"
                     /old_locus_tag="pgaptmp_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4561633.1"
                     /translation="NKNSSTNNFREISETSTRSPITLDGWISCTERMPEKSQNVLISM
                     NIDSEAGPLIYSARYLGGTFRRGGIAVSPGNDLRQATHWMSLPEPPQEVNQ"
BASE COUNT          104 a           84 c          110 g           86 t
ORIGIN      
        1 aaaataagaa ttcgtcaacc aacaattttc gggaaatctc ggaaacgtca accagatctc
       61 cgataactct ggatggctgg ataagctgta ctgagcgaat gcctgaaaag agccagaacg
      121 tgcttatttc gatgaatatc gatagcgagg ctgggccatt aatatattcc gcacgctatc
      181 tcggaggcac gttccggcgc ggaggtatag cagttagtcc gggtaatgat cttaggcaag
      241 caacccactg gatgtcgcta ccagaaccgc cgcaggaggt gaatcaatga cctggcctga
      301 agcattcaca acggtaggaa ttgcgatggc ggtggcgctg gtggtgtatt cgatttgccg
      361 ctggggataa aaacggtttg cggg
//
LOCUS       DAZKHC020000473          362 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZKHC020000473 DAZKHC020000000
VERSION     DAZKHC020000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.479"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>362
                     /locus_tag="ACHOSM_RS27030"
                     /old_locus_tag="pgaptmp_005406"
     CDS             <1..>362
                     /locus_tag="ACHOSM_RS27030"
                     /old_locus_tag="pgaptmp_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4561634.1"
                     /translation="ARVKGSQLAKDPEVLAFIARKQCETVEVDEVPVYRQKKSEQEDK
                     PRRREAAAIPQPDENNPEMPPPAVISHGIEYMEDGLPDPVKAMGQILVENLIIDPKLA
                     LDAAWRLAQFTHHKKGDA"
BASE COUNT           97 a           86 c          112 g           67 t
ORIGIN      
        1 agccagggtt aaaggctcgc agctggcaaa agatccggag gtgcttgcgt ttatagcccg
       61 taaacagtgc gagacggtgg aggtggatga ggttcctgtt taccggcaga aaaaatcaga
      121 gcaggaggat aaaccccgtc gccgtgaggc ggctgcaata ccacagccgg acgaaaacaa
      181 tccggagatg cctccgcccg cggtgatatc tcatggtatt gaatatatgg aggatggtct
      241 tcccgatccg gtgaaagcta tggggcagat cctggtggaa aacctgataa ttgacccgaa
      301 actggcactg gatgcggcct ggcgactggc gcagttcaca caccataaaa aaggcgatgc
      361 cg
//
LOCUS       DAZKHC020000474          358 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZKHC020000474 DAZKHC020000000
VERSION     DAZKHC020000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.480"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>358
                     /locus_tag="ACHOSM_RS27035"
                     /old_locus_tag="pgaptmp_005407"
     CDS             <1..>358
                     /locus_tag="ACHOSM_RS27035"
                     /old_locus_tag="pgaptmp_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4561635.1"
                     /translation="ALTNNQFGLGIYLYTDAPVPDGVAMTGTGNPFLMSYFTQTTDGR
                     VNLMHHRKAGNTKLGEFGDYGNDWQTLELVFTAGSATVTPKLNGVAGPAFQVIKDSLT
                     LGLNALTLTDVTKNAAY"
BASE COUNT           87 a           86 c          108 g           77 t
ORIGIN      
        1 cgcactgacc aacaatcagt tcgggctggg gatttatctg tatacggatg ctcccgttcc
       61 tgatggtgtg gcgatgacgg gtaccggtaa tccgttcctg atgtcgtact tcactcagac
      121 cactgacggc agagtgaatc tgatgcatca caggaaagcc ggaaacacga agctggggga
      181 gttcggcgat tacggtaacg actggcagac gctggagctg gtgttcaccg ccggcagtgc
      241 cacggttact ccgaaactga atggagtggc tggcccggca ttccaggtta taaaagacag
      301 tctgacactg ggactgaatg cgctgacgct gacggatgtt acaaaaaatg cagcgtat
//
LOCUS       DAZKHC020000475          351 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZKHC020000475 DAZKHC020000000
VERSION     DAZKHC020000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.481"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>351
                     /locus_tag="ACHOSM_RS27040"
                     /old_locus_tag="pgaptmp_005408"
     CDS             <1..>351
                     /locus_tag="ACHOSM_RS27040"
                     /old_locus_tag="pgaptmp_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4561636.1"
                     /translation="RDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIARRWHASNKQ
                     WAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRIS
                     AVMRYAVQSGIIRY"
BASE COUNT          111 a           88 c           73 g           79 t
ORIGIN      
        1 acgggatgag gcccgaaaac tgatttcgga gggggttgac ccttgcgaac agaaaagagc
       61 taaaaaagta gtccctgatt tacaactctc tttcgaacat attgcacgac gctggcatgc
      121 cagtaataaa caatgggcac aatcacacag cgataaagta ctcaaaagcc tcgagacaca
      181 cgtattcccc tttatcggca atcgggatat cacaacactc aataccccgg acctgcttat
      241 ccctgttcgt gctgcagaag ctaaacaaat ttatgaaatc gccagtcgtc tgcagcaaag
      301 aatatctgcc gtaatgcgtt atgccgtaca gtctggcatc atcagatata a
//
LOCUS       DAZKHC020000476          349 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZKHC020000476 DAZKHC020000000
VERSION     DAZKHC020000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.482"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT           99 a           77 c           82 g           91 t
ORIGIN      
        1 gacgacacgg tttgtagtta cgttccttcg gtgagtgagt acctgtgcgt cagggtaacg
       61 caatcagttc aggtttagcg gggtgtcaag tcggtttggc taaccgaccg cagaattaac
      121 tgtaccttta aaggtcgccc ccgatatatc tgcctgtcag acatatcgcc gattcggtat
      181 ctcactacat aaaactcctt aaaaaaacaa tgcgattctg ttggcacgaa gctttcagga
      241 agtcagtccg gagaagcgcc agtacgatac agaatgaggt tgaaaaaaac gttcacgtct
      301 ggtcacttct tcaaatgtta aaaaacatct cgtaaagagc cagtttatg
//
LOCUS       DAZKHC020000477          343 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZKHC020000477 DAZKHC020000000
VERSION     DAZKHC020000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.483"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>343
                     /locus_tag="ACHOSM_RS27045"
                     /old_locus_tag="pgaptmp_005409"
     CDS             <1..>343
                     /locus_tag="ACHOSM_RS27045"
                     /old_locus_tag="pgaptmp_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4561637.1"
                     /translation="VHPGGVPALSGTGARAGDLKGINVKYRYEFTDHLGGIVALSYAS
                     VKKSDTMKTGENTFHYESLRGRYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDY
                     RRDEVKPGYVK"
BASE COUNT           76 a           74 c          114 g           79 t
ORIGIN      
        1 aggttcaccc gggcggcgta ccggcattgt ccggtaccgg tgcgcgtgca ggtgatttaa
       61 aagggattaa tgtgaaatac cgttatgagt tcacggatca cctgggcggc attgtcgcgc
      121 tgagttatgc atcggtgaag aaaagtgaca cgatgaagac gggtgaaaat accttccatt
      181 atgaaagcct gcgcggtcgt tatgtcagtc tgatggccgg ccctgtctgg cagctcagtg
      241 agcgggtcag tctctatggc atggccggga tggcgtacac ccgctggtct gacagtgttc
      301 aggattaccg gcgtgatgaa gtgaaaccgg ggtatgtgaa gga
//
LOCUS       DAZKHC020000478          342 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZKHC020000478 DAZKHC020000000
VERSION     DAZKHC020000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.484"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>342
                     /locus_tag="ACHOSM_RS27050"
                     /old_locus_tag="pgaptmp_005410"
     CDS             <1..>342
                     /locus_tag="ACHOSM_RS27050"
                     /old_locus_tag="pgaptmp_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4561638.1"
                     /translation="ARNAGISASQAEENAANADTSAGEASESARQAAESAASAKKSEE
                     ASSSSASEAAQKASESLQSATDAELSKKTAESAAGNAARDATTAAEKARESAESAQSA
                     EQSRIAAEEAVN"
BASE COUNT          110 a           85 c          107 g           40 t
ORIGIN      
        1 gcgaggaatg ccggcatatc agccagtcag gcagaagaga acgctgcaaa tgctgacact
       61 tcagcagggg aggcatcgga gtcagcccgg caggcggcag aaagtgcagc ctctgcaaag
      121 aagtcagagg aagcgtcctc gtcctcagcc tctgaggccg ctcaaaaagc cagtgagtca
      181 ttacaaagtg caacagatgc tgagttgtca aaaaagacgg cagaaagtgc agccggtaat
      241 gcagccaggg atgcaacgac cgcagcagaa aaagcccggg agtcagcaga aagcgcacag
      301 tcagcggaac aaagcaggat agcggcggaa gaagccgtaa ac
//
LOCUS       DAZKHC020000479          334 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZKHC020000479 DAZKHC020000000
VERSION     DAZKHC020000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.485"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            50..244
                     /locus_tag="ACHOSM_RS27055"
                     /old_locus_tag="pgaptmp_005411"
     CDS             50..244
                     /locus_tag="ACHOSM_RS27055"
                     /old_locus_tag="pgaptmp_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HGU4561639.1"
                     /translation="MTFTVKTIPDMLVEAYENQTEVARILNCSRNTVRKYTGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            237..>334
                     /locus_tag="ACHOSM_RS27060"
                     /old_locus_tag="pgaptmp_005412"
     CDS             237..>334
                     /locus_tag="ACHOSM_RS27060"
                     /old_locus_tag="pgaptmp_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4561640.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKG"
BASE COUNT          103 a           66 c           93 g           72 t
ORIGIN      
        1 agtatcagca gaaacttaaa gacctgcgaa acagcagaag tgaggccgca tgacgttcac
       61 agtaaaaacc attcctgaca tgctcgttga ggcatatgaa aatcagaccg aggtagccag
      121 aatactgaac tgtagtcgca acacggtcag aaaatacact ggcgataaag aaggaaaaag
      181 acacgctatt gtcaacggtg ttcttatggt tcatcgcgga tggggtaaag atactgatgc
      241 gtgatatccg gcaggttctt gagcgctggg gtgcatgggc ggcaaataac tatgaggatg
      301 ttacatggtc gcccattgct gccggattta aggg
//
LOCUS       DAZKHC020000480          328 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZKHC020000480 DAZKHC020000000
VERSION     DAZKHC020000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.486"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          128 a           35 c           63 g          102 t
ORIGIN      
        1 gtatgtgcaa tcgaatttta tcagttgctt taatggatga gagtaaatta gactcatata
       61 tacattgtat acgaggaata aaagacggag taaaacttgg gaaaaatgaa aaaaatatta
      121 atgatatgca ctaagtatcc actagacctc aatagtacgt ggttaactag agaacttgct
      181 gaagaattta ataacagggg gtacgtgatt gatgtaatct gtattgattg gggtgtttta
      241 caaaagagaa aaaaaataag cttaaataac atcaatatat ataatgtccc tgctttaggt
      301 tgtaaaaaaa cgagcttttt aaattttt
//
LOCUS       DAZKHC020000481          326 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZKHC020000481 DAZKHC020000000
VERSION     DAZKHC020000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.487"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          135 a           69 c           36 g           86 t
ORIGIN      
        1 gtgatcatgt agcataagta acctccgttt atctacagac tgccatcctg aatttaccgt
       61 ccttaataag acaacaataa aaaactaatc atataaaaac aataaaaata atatacaaaa
      121 aaactaaaca acaaagaaaa gcaaatcata tctggcattt taattgaaag agcaattacc
      181 gaacaaaaga cgctgactat atactcaaaa ccaaacaaca attctgccaa tcaggtatca
      241 tggcaacaca cggaattacc atgtttttgc cttctctgcc catacaatac gggcatatac
      301 ttcatactct attgtaatat ttctat
//
LOCUS       DAZKHC020000482          319 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZKHC020000482 DAZKHC020000000
VERSION     DAZKHC020000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.denovo.488"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>319
                     /locus_tag="ACHOSM_RS27065"
                     /old_locus_tag="pgaptmp_005413"
     CDS             <1..>319
                     /locus_tag="ACHOSM_RS27065"
                     /old_locus_tag="pgaptmp_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4561641.1"
                     /translation="KHKEHMDDPLFHLIRSNTLKPTPVYANYFDNTTDFRPARERIFI
                     CGETLKGEDPSKYIRQKYGHHGPGVDQSQQFDNGIMGLNSLKEARDKNNKIIYSSGLS
                     CFQS"
BASE COUNT          112 a           62 c           59 g           86 t
ORIGIN      
        1 aaacataaag aacacatgga tgatcctcta ttccatctaa tccgaagcaa tactttgaaa
       61 cccacacctg tatatgctaa ttattttgat aacactactg atttcagacc agcacgtgaa
      121 cgtatcttca tatgtgggga gacactcaaa ggtgaagacc ccagcaaata cattagacaa
      181 aaatatgggc atcatgggcc tggggtagat cagagtcaac aatttgataa tggtattatg
      241 gggctaaatt ccctgaagga agcacgtgat aaaaataata aaataattta ttcctcaggg
      301 cttagttgtt ttcaatccc
//
LOCUS       DAZKHC020000483         6072 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.guided.182, whole genome shotgun sequence.
ACCESSION   DAZKHC020000483 DAZKHC020000000
VERSION     DAZKHC020000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.guided.182"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..624
                     /locus_tag="ACHOSM_RS27070"
                     /old_locus_tag="pgaptmp_005414"
     CDS             <1..624
                     /locus_tag="ACHOSM_RS27070"
                     /old_locus_tag="pgaptmp_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HGU4561642.1"
                     /translation="GGGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINR
                     QMIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRG
                     DYSWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTVTQLSRTELGSRVICRDK
                     QLIFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            621..1235
                     /gene="prfH"
                     /locus_tag="ACHOSM_RS27075"
                     /old_locus_tag="pgaptmp_005415"
     CDS             621..1235
                     /gene="prfH"
                     /locus_tag="ACHOSM_RS27075"
                     /old_locus_tag="pgaptmp_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HGU4561643.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLEIET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1292..2749)
                     /gene="pepD"
                     /locus_tag="ACHOSM_RS27080"
                     /old_locus_tag="pgaptmp_005416"
     CDS             complement(1292..2749)
                     /gene="pepD"
                     /locus_tag="ACHOSM_RS27080"
                     /old_locus_tag="pgaptmp_005416"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU4561644.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3010..3468
                     /gene="gpt"
                     /locus_tag="ACHOSM_RS27085"
                     /old_locus_tag="pgaptmp_005417"
     CDS             3010..3468
                     /gene="gpt"
                     /locus_tag="ACHOSM_RS27085"
                     /old_locus_tag="pgaptmp_005417"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU4561645.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3560..4825
                     /gene="frsA"
                     /locus_tag="ACHOSM_RS27090"
                     /old_locus_tag="pgaptmp_005418"
     CDS             3560..4825
                     /gene="frsA"
                     /locus_tag="ACHOSM_RS27090"
                     /old_locus_tag="pgaptmp_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU4561646.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYY
                     SLYERYFAPRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAA
                     FGFRFGANVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGM
                     HDASDEALRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGK
                     LLEIPFNPVYRNFDKGLQEITGWIEKRLC"
     gene            4883..5284
                     /gene="crl"
                     /locus_tag="ACHOSM_RS27095"
                     /old_locus_tag="pgaptmp_005419"
     CDS             4883..5284
                     /gene="crl"
                     /locus_tag="ACHOSM_RS27095"
                     /old_locus_tag="pgaptmp_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU4561647.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVIERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5323..>6072)
                     /locus_tag="ACHOSM_RS27100"
                     /old_locus_tag="pgaptmp_005420"
     CDS             complement(5323..>6072)
                     /locus_tag="ACHOSM_RS27100"
                     /old_locus_tag="pgaptmp_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU4561648.1"
                     /translation="KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTK
                     RASGLATYRNTDFFGVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAI
                     SGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFA
                     NKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSA
                     FVDYKINQLDSDNKLNINNDDIVAVGMTYQF"
BASE COUNT         1466 a         1488 c         1605 g         1513 t
ORIGIN      
        1 gggggggggc agtctatttt acagcggcat attgcctcgt tctcgcatca tggtttgcct
       61 caaggcagtg acgacgcgct acggtatatt gcggaacatg acgatgcgct ggcgtttgcg
      121 tgtattaatc gccagatgat cgctttgcgc ataatgcaac aagttaaggc cacgggaagt
      181 ccggttctgg atgtggcgca taactttgtt agcgcgtgtc aaatcggcga tcaacagggc
      241 tggttgcatc gtaaaggtgc cacgccggat gacaacggtc tggtgattat tcccggttca
      301 cgtggtgatt actcctggat tgtacagcct gttgcgaacg aaaaaacgct ccactcgctg
      361 gcgcatgggg cagggcgtaa atgggggcgc accgagtgta aagggcgtct ggcagcgaaa
      421 tacacggtga cgcaactctc gcggactgaa cttggcagtc gggtaatttg tcgcgataaa
      481 caactcatct ttgaagaagc gccacaagcc tataaatcgg ctgaaagcgt ggtgcaatgt
      541 ctggtgcagg ccggtttaat tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa
      601 aacagtggag ggaaaaaagg atgatcttgc tacaactctc ctcagcgcag gggccggaag
      661 aatgttgtct cgcagtgaaa aaggcgctgg acaggctaat taaagaagct gcccgacaag
      721 acgtcgcggt aacggtgttg gaaatagaaa cgggccgcta ctctgacacg ctgcgttcag
      781 ctctggtttc tctggatggc gataacgcat gggcattaag cgaaagctgg tgcggcacta
      841 ttcagtggat ttgtccgagt ccgtatcggc ctcatcatgg gcgcaaaaac tggtttctgg
      901 gcattgggcg ttttaccgct gatgagcagg aacaatcgga tgcaatccgt tatgagacgt
      961 tgcgttcgtc ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca
     1021 cgcatttggc atccggtatt agcgtgaagg ttcagtcaga gcgtagtcag catgctaaca
     1081 agcggctggc acgattgctg attgcctgga agctggagca gcagcaacag gaaaatagcg
     1141 cggtgctgaa atcgcagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgagac
     1201 ggacatttac agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg
     1261 attaaacgcc atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt
     1321 cagtcagcag cgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt
     1381 gtggaccggt gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga
     1441 acagaccaca ttccaggccc gcgtggataa tctggatgtt cggtgttttg ttgaacagac
     1501 gctgataggt ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag
     1561 gatatgcgcc tttcgcttcg gttttcgcgc cagctagttt acccagcgaa tccagcatgc
     1621 tcaccacgta gtctttaccg ctgtcgatca gtgaacggat caggcagtga atttctacgt
     1681 tattgtcagt catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca
     1741 cgtcggaatt gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc
     1801 gagatttcgc aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat
     1861 ttttctcttt ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt
     1921 tcaggacgtc gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg
     1981 cgttacgcag tgtgccgccg ttgaaatcga taaggcgcag atccagttct tccgcatgac
     2041 ccgccaggaa gcgcaccagt agtttgttgg cattacccag gccaacgtgg atttcaccgc
     2101 cggagtgacc gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa
     2161 ccgcttcacg atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt
     2221 agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc
     2281 tctgtaagcc gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca
     2341 gcgggccgtg aaccacgttt tcgtcagcca gaactgccag tgcagaggcc ataccaatgc
     2401 cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct
     2461 ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga
     2521 ggtgggcctg taagacgacc ggtttacgat tttccatacc tgcggtagca ggtttacgaa
     2581 tcaggatatt acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa
     2641 caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg
     2701 caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc
     2761 ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac
     2821 acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc
     2881 cgtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
     2941 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
     3001 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
     3061 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
     3121 ggcggtctgg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     3181 gtttgtattt ccagctacga tcacgacaac cagcgagagc ttaaagtgct gaaacgcgca
     3241 gaaggcgatg gcgaaggctt catagttatt gatgacctgg tggataccgg tggtactgct
     3301 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
     3361 ggtcgtccgc tggttgatga ctatgttgtt gatattccgc aagatacctg gatcgaacag
     3421 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
     3481 ctggcactgc cgggcgttgt tctttttaac ttcaggcagg ttacaatagt ttccagtaag
     3541 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
     3601 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
     3661 tgtgcagtcg gctcttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
     3721 gatgtggatc tggcgcggca ttgacccacg tgaaatcctc gacgtccagg cgcgtattgt
     3781 gatgagcgat gccgaacgta ccgacgatga tttatacgat accgtgattg gctaccgtgg
     3841 tggcaactgg atttatgagt gggccaccca ggcgatggtg tggcaacaaa aagcctgtgc
     3901 ggaagaagat ccgcaactca gtggtcgaca ctggctgcat gcggctacgt tgtacaacat
     3961 tgccgcctat cctcatctga aaggagatga cctggccgag caagcgcagg ctttatcaaa
     4021 ccgcgcctat gaagaggccg cccagcgtct gccgggcacg atgcggcaga tggagtttac
     4081 cgtacccggc ggtgcgccca tcacccccgg cggtgcgccc atcaccggct ttttgcatat
     4141 gccgaaaggc gatggcccgt tcccgacagt attaatgtgt ggtggtctgg atgcgatgca
     4201 gacggactat tacagcctgt atgaacgtta ttttgcgccg cgcggcattg cgatgctgac
     4261 tattgatatg ccgtcggtgg gcttttcttc aaaatggaag ctcacccagg actccagtct
     4321 gttgcatcag cacgtcttaa aggcgctgcc taacgtaccg tgggtggatc acactcgcgt
     4381 cgcggccttt ggtttccgtt tcggcgctaa cgttgccgtg cgtctggcat accttgaatc
     4441 gccgcgtctg aaagcggttg cctgtcttgg tccggtagtt cataccctgt tgagtgattt
     4501 taagtgccag caacaggtgc cggaaatgta tcttgacgtt ctggccagtc gtttggggat
     4561 gcatgatgct tccgatgaag cgttgcgcgt ggagctgaat cgctattcat taaaagtgca
     4621 aggattgctg ggacgtcgct gcccaacgcc aatgttatca ggctactgga agaacgatcc
     4681 gttcagcccg gaagaggact cacgcttaat cacctcatca tctgctgacg gtaaattatt
     4741 agagatccca tttaacccgg tgtatcggaa ttttgacaaa ggtcttcagg aaatcaccgg
     4801 ctggatcgaa aaacgcttgt gttaaaaatt tgctaaattt tgccaatttg gtaaaacagt
     4861 tgcatcacaa caggagatag caatgacgtt accgagtgga cacccgaaga gcagattgat
     4921 caaaaaattt accgcactag gcccgtatat tcgtgaaggt aagtgcgaag ataatcgatt
     4981 ctttttcgat tgtctggctg tatgcgtcaa cgtgaaaccg gcaccggaag tgcgtgaatt
     5041 ctggggctgg tggatggagc tggaagcgca ggaatcccgt tttacctaca gctaccagtt
     5101 tggtctgttc gataaagcag gcgactggaa gagtgttccg gtaaaagata ctgaagtgat
     5161 tgaacgactg gagcacaccc tgcgtgagtt tcacgagaag ctgcgtgaac tgctgacgac
     5221 gctgaatctg aagctggaac cggcggatga ttttcgtgac gagccggtga agttaacggc
     5281 gtgagtgaaa tgtgccggat gcatcacatc cggcaataat cattaaaact gataggtcat
     5341 gccaaccgcg acaatatcat cattattaat attcaatttg ttatcgctat ccagttggtt
     5401 gattttataa tcaacaaacg ctgacatatt tttgttgaaa taatatgtag ccccgacatc
     5461 gatataattg accagatctt catcaccgat accttcaata tctttccctt tcgataagac
     5521 ataacccagc gatggacgca gaccaaagtc aaactggtat tgagcgaccg cttcaaagtt
     5581 ctgagtttta ttggcaaagc cgccagatat tggcgtcatt ttgcgtgttt cagaatagaa
     5641 agttgccaga taaatattat tggcatcgta tttcagacct gttgcccatg cttctgcacg
     5701 cttgcctgtg ccacggcttt gcaggttctg ctcgttggtg cgatctgagt tggtataggc
     5761 cccactaatg gcgaaatcgc tgccgccaaa gtcatatgtc aatgacgtgc cgaagccatc
     5821 gccgttttgc tttttaacgt cgcggttttc gtttttccct tgatattgca gggttaagtt
     5881 caggccatcg ataacgccga agaagtcggt gttccgatat gtcgccagac cgctggcgcg
     5941 tttggtcata aagttgtcgg tctgcgccga ggagtcgcca ccaaattccg ggaacatatc
     6001 ggtccaggct tccacgtcat acaacgcgcc caggttacga ccataatcga aagaacccaa
     6061 atctttatat tt
//
LOCUS       DAZKHC020000484         2090 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.guided.276, whole genome shotgun sequence.
ACCESSION   DAZKHC020000484 DAZKHC020000000
VERSION     DAZKHC020000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.guided.276"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            50..409
                     /gene="frdD"
                     /locus_tag="ACHOSM_RS27105"
                     /old_locus_tag="pgaptmp_005421"
     CDS             50..409
                     /gene="frdD"
                     /locus_tag="ACHOSM_RS27105"
                     /old_locus_tag="pgaptmp_005421"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU4561649.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            472..1605
                     /locus_tag="ACHOSM_RS27110"
                     /old_locus_tag="pgaptmp_005422"
     CDS             472..1605
                     /locus_tag="ACHOSM_RS27110"
                     /old_locus_tag="pgaptmp_005422"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU4561650.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1694..>2090
                     /gene="blc"
                     /locus_tag="ACHOSM_RS27115"
                     /old_locus_tag="pgaptmp_005423"
     CDS             1694..>2090
                     /gene="blc"
                     /locus_tag="ACHOSM_RS27115"
                     /old_locus_tag="pgaptmp_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU4561651.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCG"
BASE COUNT          503 a          533 c          537 g          517 t
ORIGIN      
        1 gccaccatcg taatcctgtt tgttgccctg tactggtaag gagcctgaga tgattaatcc
       61 aaatccaaag cgttctgacg aaccggtatt ctggggcctc ttcggggccg gtggtatgtg
      121 gagcgcaatt attgcgccgg tgatgatcct gctggtgggt attctgctgc cactggggct
      181 gtttccgggt gatgcgctga gctacgagcg cgttctggcg ttcgcgcaga gcttcattgg
      241 tcgcgtattc ctgttcctga tgatcgttct gccgctgtgg tgtggtttac accgtatgca
      301 ccacgcgatg cacgatctga aaattcacgt gcctgcgggc aaatgggttt tctacggtct
      361 ggctgctatc ctgacagttg tcacgctgat tggtatcgtt acaatctaac gtatcgccaa
      421 tgtaaatccg gcccgcctat ggcgggccgt tttgtatgga aaccagacct tatgttcaaa
      481 acgacgctct gcgccttatt aattaccgcc tcttgctcca catttgctgc ccctcaacaa
      541 atcaacgata ttgtgcatcg cacaattacc ccgcttatag agcaacaaaa gatccccggt
      601 atggcggtgg cggtaattta tcagggtaaa ccttattact ttacctgggg ctatgcggac
      661 atcgccaaaa agcagcccgt cacacagcaa acgttgtttg agttaggttc ggtcagcaaa
      721 acatttacgg gcgtgcttgg tggcgacgct attgctcgag gggaaatcaa gttaagcgat
      781 cccacaacaa aatactggcc tgaacttacc gctaaacagt ggaatgggat cacactatta
      841 catctcgcaa cctacactgc tggcggcctg ccattgcagg tgccggatga ggtgaaatcc
      901 tcaagcgact tgctgcgctt ctatcaaaac tggcagcctg catgggcgcc aggaacacaa
      961 cgtctgtatg ccaactccag tatcggtttg ttcggcgcac tggctgtgaa gccgtctggt
     1021 ttgagttttg agcaggcgat gcaaacacgt gtcttccagc cactcaaact caaccatacg
     1081 tggattaatg taccgcccgc agaagaaaag aattacgcct ggggatatcg cgaaggcaag
     1141 gcagttcatg tttcgccagg ggcgttagat gctgaaactt atggtgtgaa gtcgaccatt
     1201 gaagatatgg cctgctgggt acgaagcaat atgaatcccc gtgatatcaa cgacaaaaca
     1261 cttcagcaag ggatacaact ggcacaatct cgctactggc aaaccggcga tatgtatcag
     1321 ggcctgggct gggaaatgct ggactggccg gtaaatcctg acagcatcat taacggcagt
     1381 ggcaataaaa ttgcactggc agcacacccc gtaaaagcga ttacgccccc aactcctgca
     1441 gtacgcgcat catgggtaca taaaacaggg gcaaccggcg gatttggtag ctatgtcgcg
     1501 tttattccag aaaaagagct gggtatagtg atgctggcta acaaaaacta tcccaatcca
     1561 gcgagagtcg ccgccgcctg gcagattctc aacgctctac agtaaaattt catcgggtcc
     1621 gaattttcgg accttttctg cgcttttcct tgctgtcatc tacacttaga aaaaaaccag
     1681 taaggaaaca tttatgcgcc tgctccctct cgttgccgca gcgacagctg catttctggt
     1741 cgttgcctgc agttctccta cgccgccgcg tggcgtaaca gtagtgaata atttcgacgc
     1801 caaacgttat ctcggtacct ggtatgagat tgcccgtttt gatcaccgtt ttgaacgtgg
     1861 actggaaaaa gtcaccgcaa catacagcct gcgtgatgac ggcggcctga atgtcattaa
     1921 taaaggctat aaccctgaca gaggaatgtg gcagcagagt gaagggaaag cgtactttac
     1981 cggcgcacca actcgcgctg cgctgaaagt gtcattcttt ggtcctttct atggcggtta
     2041 taacgttatt gcactcgatc gggaataccg ccatgcgctg gtttgcggcc
//
LOCUS       DAZKHC020000485         1347 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.guided.331, whole genome shotgun sequence.
ACCESSION   DAZKHC020000485 DAZKHC020000000
VERSION     DAZKHC020000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.guided.331"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..965
                     /locus_tag="ACHOSM_RS27120"
                     /old_locus_tag="pgaptmp_005424"
     CDS             6..965
                     /locus_tag="ACHOSM_RS27120"
                     /old_locus_tag="pgaptmp_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU4561652.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            977..1246
                     /locus_tag="ACHOSM_RS27125"
                     /old_locus_tag="pgaptmp_005425"
     CDS             977..1246
                     /locus_tag="ACHOSM_RS27125"
                     /old_locus_tag="pgaptmp_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HGU4561653.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          378 a          259 c          328 g          382 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggc ttttcttcgg
       61 tatcctattc ccgggaattt acgatagact tttcgactca acaaagttat gtatcttcgt
      121 taaatagtat acggacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta cagattttca gattttgcac
      361 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcggc gatactgggc actgtggccg
      781 ttatactgaa ttgtcatcat cagggggcgc gttctgttcg cgccgtgaat gaagagagtc
      841 aaccagaatg tcagataact ggcgacaggc ccgttataaa aataaacaat acattatggg
      901 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
      961 aataaaggag ttaagtatga agaagatgtt tatggcggtt ttatttgcat tagtttctgt
     1021 taatgcaatg gcggcggatt gcgctaaagg taaaattgag ttttccaagt ataatgagaa
     1081 tgatacattc acagtaaaag tggccggaaa agagtactgg accagtcgct ggaatctgca
     1141 accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat ccagtacctg
     1201 tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca taacctgatt
     1261 cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga cagttgcctg
     1321 tcagactgag catttgttaa aaaaatt
//
LOCUS       DAZKHC020000486         1347 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.guided.332, whole genome shotgun sequence.
ACCESSION   DAZKHC020000486 DAZKHC020000000
VERSION     DAZKHC020000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.guided.332"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            6..965
                     /locus_tag="ACHOSM_RS27130"
                     /old_locus_tag="pgaptmp_005426"
     CDS             6..965
                     /locus_tag="ACHOSM_RS27130"
                     /old_locus_tag="pgaptmp_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU4561654.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            977..1246
                     /locus_tag="ACHOSM_RS27135"
                     /old_locus_tag="pgaptmp_005427"
     CDS             977..1246
                     /locus_tag="ACHOSM_RS27135"
                     /old_locus_tag="pgaptmp_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HGU4561655.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          376 a          261 c          328 g          382 t
ORIGIN      
        1 tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggt ttttcttcgg
       61 tatcctattc ccgggagttt acgatagact tttcgaccca acaaagttat gtctcttcgt
      121 taaatagtat acggacagag atatcgaccc ctcttgaaca tatatctcag gggaccacat
      181 cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat atacgagggc
      241 ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa aataatttat
      301 atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca gattttacac
      361 atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt tataccactc
      421 tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac tcactggttt
      481 catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat gcatccagag
      541 cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag atacagagag
      601 aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg ggagacgtgg
      661 acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg ggagaggatg
      721 gtgtcagagt ggggagaata tcctttaata atatatcagc gatactgggg actgtggccg
      781 ttatactgaa ttgccatcat cagggggcgc gttctgttcg cgccgtgaat gaagagagtc
      841 aaccagaatg tcagataact ggcgacaggc ccgttataaa aataaacaat acattatggg
      901 aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat acaacgggta
      961 aataaaggag ttaagtatga agaagatgtt tatggcggtt ttatttgcat tagtttctgt
     1021 taatgcaatg gcggcggatt gcgctaaagg taaaattgag ttttccaagt ataatgagaa
     1081 tgatacattc acagtaaaag tggccggaaa agagtactgg accagtcgct ggaatctgca
     1141 accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat ccagtacctg
     1201 tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca taacctgatt
     1261 cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga cagttgcctg
     1321 tcagactgag catttgttaa aaaaatt
//
LOCUS       DAZKHC020000487         1342 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.guided.334, whole genome shotgun sequence.
ACCESSION   DAZKHC020000487 DAZKHC020000000
VERSION     DAZKHC020000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.guided.334"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..960
                     /locus_tag="ACHOSM_RS27140"
                     /old_locus_tag="pgaptmp_005428"
     CDS             <1..960
                     /locus_tag="ACHOSM_RS27140"
                     /old_locus_tag="pgaptmp_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU4561656.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            972..1241
                     /locus_tag="ACHOSM_RS27145"
                     /old_locus_tag="pgaptmp_005429"
     CDS             972..1241
                     /locus_tag="ACHOSM_RS27145"
                     /old_locus_tag="pgaptmp_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU4561657.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          375 a          259 c          329 g          379 t
ORIGIN      
        1 atgaagtgta tattatttaa atgggtactg tgcctgttac tgggcttttc ttcggtatcc
       61 tattcccggg aatttacgat agacttttcg actcaacaaa gttatgtatc ttcgttaaat
      121 agtatacgga cagagatatc gacccctctt gaacatatat ctcaggggac cacatcggtg
      181 tctgttatta accacacccc accgggcagt tattttgctg tggatatacg agggcttgat
      241 gtctatcagg cgcgttttga ccatcttcgt ctgattattg agcaaaataa tttatatgtg
      301 gccgggttcg ttaatacggc aacaaatact ttctacagat tttcagattt tgcacatata
      361 tcagtgcccg gtgtgacaac ggtttccatg acaacggaca gcagttatac cactctgcaa
      421 cgtgtcgcag cgctggaacg ttccggaatg caaatcagtc gtcactcact ggtttcatca
      481 tatctggcgt taatggagtt cagtggtaat acaatgacca gagatgcatc cagagcagtt
      541 ctgcgttttg tcactgtcac agcagaagcc ttacgcttca ggcagataca gagagaattt
      601 cgtcaggcac tgtctgaaac tgctcctgtg tatacgatga cgccgggaga cgtggacctc
      661 actctgaact gggggcgaat cagcaatgtg cttccggagt atcggggaga ggatggtgtc
      721 agagtgggga gaatatcctt taataatata tcggcgatac tgggcactgt ggccgttata
      781 ctgaattgtc atcatcaggg ggcgcgttct gttcgcgccg tgaatgaaga gagtcaacca
      841 gaatgtcaga taactggcga caggcccgtt ataaaaataa acaatacatt atgggaaagt
      901 aatacagctg cagcgtttct gaacagaaag tcacagtttt tatatacaac gggtaaataa
      961 aggagttaag catgaagaag atgtttatgg cggttttatt tgcattagct tctgttaatg
     1021 caatggcggc ggattgtgct aaaggtaaaa ttgagttttc caagtataat gaggatgaca
     1081 catttacagt gaaggttgac gggaaagaat actggaccag tcgctggaat ctgcaaccgt
     1141 tactgcaaag tgctcagttg acaggaatga ctgtcacaat caaatccagt acctgtgaat
     1201 caggctccgg atttgctgaa gtgcagttta ataatgactg aggcataacc tgattcgtgg
     1261 tatgtgggta acaagtgtaa tctgtgtcac aattcagtca gttgacagtt gcctgtcaga
     1321 ctgagcattt gttaaaaaaa tt
//
LOCUS       DAZKHC020000488         1342 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2107S21285
            SAMN43267538-rid25106513.guided.335, whole genome shotgun sequence.
ACCESSION   DAZKHC020000488 DAZKHC020000000
VERSION     DAZKHC020000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267538
            Sequence Read Archive: SRR30307398
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350716.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350716.2
            Genome Coverage       :: 66x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 17:12:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,431
            CDSs (total)                      :: 5,339
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 295
            CDSs (without protein)            :: 295
            Pseudo Genes (ambiguous residues) :: 0 of 295
            Pseudo Genes (frameshifted)       :: 112 of 295
            Pseudo Genes (incomplete)         :: 166 of 295
            Pseudo Genes (internal stop)      :: 61 of 295
            Pseudo Genes (multiple problems)  :: 39 of 295
            Pseudo Genes (short protein)      :: 1 of 295
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267538-rid25106513.guided.335"
                     /serotype="O177:H25"
                     /isolate="2107S21285"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-07"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..960
                     /locus_tag="ACHOSM_RS27150"
                     /old_locus_tag="pgaptmp_005430"
     CDS             <1..960
                     /locus_tag="ACHOSM_RS27150"
                     /old_locus_tag="pgaptmp_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HGU4561658.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            972..1241
                     /locus_tag="ACHOSM_RS27155"
                     /old_locus_tag="pgaptmp_005431"
     CDS             972..1241
                     /locus_tag="ACHOSM_RS27155"
                     /old_locus_tag="pgaptmp_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HGU4561659.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          373 a          261 c          329 g          379 t
ORIGIN      
        1 atgaagtgta tattatttaa atgggtactg tgcctgttac tgggtttttc ttcggtatcc
       61 tattcccggg agtttacgat agacttttcg acccaacaaa gttatgtctc ttcgttaaat
      121 agtatacgga cagagatatc gacccctctt gaacatatat ctcaggggac cacatcggtg
      181 tctgttatta accacacccc accgggcagt tattttgctg tggatatacg agggcttgat
      241 gtctatcagg cgcgttttga ccatcttcgt ctgattattg agcaaaataa tttatatgtg
      301 gccgggttcg ttaatacggc aacaaatact ttctaccgtt tttcagattt tacacatata
      361 tcagtgcccg gtgtgacaac ggtttccatg acaacggaca gcagttatac cactctgcaa
      421 cgtgtcgcag cgctggaacg ttccggaatg caaatcagtc gtcactcact ggtttcatca
      481 tatctggcgt taatggagtt cagtggtaat acaatgacca gagatgcatc cagagcagtt
      541 ctgcgttttg tcactgtcac agcagaagcc ttacgcttca ggcagataca gagagaattt
      601 cgtcaggcac tgtctgaaac tgctcctgtg tatacgatga cgccgggaga cgtggacctc
      661 actctgaact gggggcgaat cagcaatgtg cttccggagt atcggggaga ggatggtgtc
      721 agagtgggga gaatatcctt taataatata tcagcgatac tggggactgt ggccgttata
      781 ctgaattgcc atcatcaggg ggcgcgttct gttcgcgccg tgaatgaaga gagtcaacca
      841 gaatgtcaga taactggcga caggcccgtt ataaaaataa acaatacatt atgggaaagt
      901 aatacagctg cagcgtttct gaacagaaag tcacagtttt tatatacaac gggtaaataa
      961 aggagttaag catgaagaag atgtttatgg cggttttatt tgcattagct tctgttaatg
     1021 caatggcggc ggattgtgct aaaggtaaaa ttgagttttc caagtataat gaggatgaca
     1081 catttacagt gaaggttgac gggaaagaat actggaccag tcgctggaat ctgcaaccgt
     1141 tactgcaaag tgctcagttg acaggaatga ctgtcacaat caaatccagt acctgtgaat
     1201 caggctccgg atttgctgaa gtgcagttta ataatgactg aggcataacc tgattcgtgg
     1261 tatgtgggta acaagtgtaa tctgtgtcac aattcagtca gttgacagtt gcctgtcaga
     1321 ctgagcattt gttaaaaaaa tt
//
